LOCUS       DAOSOO010000001        43868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_911_length_43868_cov_5.701938, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000001 DAOSOO010000000
VERSION     DAOSOO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 43868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43868
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_43868_cov_5.701938"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLT86_00005"
     CDS             <1..357
                     /locus_tag="PLT86_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35029.1"
                     /translation="AERWGAVADQLGAENRFNQAWALVQQKLREFPENPHLLYLAGFY
                     YNRAGNKRSALEVLDRAVLTDDPTWRGLSLNLHDSIEPPLSEDEINQAKFFGRKVPRI
                     HRCRIPGREKQNEVMQ"
     gene            502..1689
                     /locus_tag="PLT86_00010"
     CDS             502..1689
                     /locus_tag="PLT86_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT35030.1"
                     /translation="MGGRKVLTIAAVSALLFGMASAQTGAPAQGGAAQSADTAKVQVS
                     FVVTQDGKETAILEKGKLVEVVATVKLNLPDAKNANVQVIADIADQLNIENEADVKVK
                     GLYIDNGSGFSLQPTSSVRPRLGFLAPEGQTGISATQRVKWVLDFMNVAPRAPFSVNA
                     AVVIPPSNEIIGQARAEQILKVVSSLTVQQLWIDGGIFMWPLGFCLIIGIAFALERMF
                     TLTFAQINTEKFFTQLATAMKDGGVEKAMEVCNATRGPVAAVLREGLLRVKQGIDHVE
                     KAIASAGAVEASFLQRGMIVIASVTVIAPFFGFLGTVWGMVIAFKAIAEAGDVNPTIV
                     ASGISQALITTVGGLIIAIISQSLYNFLLSRINRIVIDMEETSTKLVDLLIEEGMDKP
                     ASS"
     gene            1715..2179
                     /locus_tag="PLT86_00015"
     CDS             1715..2179
                     /locus_tag="PLT86_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT35031.1"
                     /translation="MLMGKGNLAPSVEIPTASMADISFLLLVFFIVSTSMNQDRGIGM
                     TLPPPGNPMPIPKENIASVWINNRGEVALKYLGQDYITVTLPEMRERIERRIIENPNL
                     VVSLLAQRDADYKTFIDVLDELRMVGSRHDWSKISIAQPFGSAGVGGGPGGQ"
     gene            2221..2649
                     /locus_tag="PLT86_00020"
     CDS             2221..2649
                     /locus_tag="PLT86_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT35032.1"
                     /translation="MALVGGKFKGPSQPEVVIPTASMADISFLLLIFFIVSTVFVRFR
                     PEFQPQLPKAESVDALKNRRNISYLWISPDGIIQIDSSLVNLNSVAGVMLKKIQKNPQ
                     LIVLIKSDEKTRYGMVSNVIEELRKANAIRIAFAAKSELE"
     gene            2665..3330
                     /locus_tag="PLT86_00025"
     CDS             2665..3330
                     /locus_tag="PLT86_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT35033.1"
                     /translation="MVGTSVKPRRDIVKTIYRPVLLVSTLVSAFLCVGFYNLNMRMEK
                     ERISSQKQGQAIEVVDIPATAQQQEQAAAPARPAVPVEARDESQVQDLTIMDTELYQQ
                     ASVAVPEKLQEASNISQQVEQEAPPMEFWMVEEKPTIVTQAIPVYPEIARQAGMEGDV
                     IVEMVIGTDGKVQSVQILRGPPVFHDAAREAAMKMTFTPAKQNDRPVRVKVSQRISFR
                     LRG"
     gene            3510..4178
                     /locus_tag="PLT86_00030"
     CDS             3510..4178
                     /locus_tag="PLT86_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT35034.1"
                     /translation="MKWFHLTLLVLFACSPIVVPEKPTIVTQAVPVYPESARQEGIEG
                     DVLVEMVVGADGMVQDVQILSGPSVFHESAREAAARMVFSPARQDHQSVRVKVLQRFS
                     YRLSSQALASRDSTAVPSLNEEIPLLEYAEVEVKPFPVGPPVPEFPEIARGVGFGGEV
                     VLDIIIGADGNVESAVVREGPPEFRGAAREAAKKMVFTPGMHGNRPVRVKAVQRIRFP
                     FSPQ"
     gene            4387..5577
                     /locus_tag="PLT86_00035"
     CDS             4387..5577
                     /locus_tag="PLT86_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35035.1"
                     /translation="MKRMFYAAVMIACSGVLGSPVRVAAQVTVVKPELTAEQALALNA
                     VRDSARTLFQQRKYRDAVEAYKRSLLIDSTDAATYFNLGLGYQVLGNDDEALAMLNRS
                     VALDSLFAPAHLEVGKVYLKKQELEQAEEAYLATKRIFADSLQYSMAADQGLNNVAIA
                     YSNRAVVGMRERNFGSAEADAEKAISLSPDISRGYVVAATVYENQQKYDEAEQRYRAA
                     VDRAENDQVKANALEGIARSILGRVRDSRNNAAGVRSARVSAAEILRQAVQIDTMNFT
                     ALVNLGNTLFDLEQYAAAESALVRAESLRPRDFRPPLKLAETYMKLNKCGEAEQAATR
                     ALAVQPGNASAHATRAEALECLGRTREALDAYVQASRDPRWKQYADRKAKTLREQLGL
                     PAQN"
     gene            5776..6756
                     /locus_tag="PLT86_00040"
     CDS             5776..6756
                     /locus_tag="PLT86_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35036.1"
                     /translation="MLVRWGFLSGAWIWPVLFAIGCGTTGPDLVAPATPAGFGRIGGG
                     DGENRFGWQRNREKDLAGYRMYRAEGDPAAAYQLIATISRDSTSYTDRNLDYTIQFYY
                     KMTAFDNAGNESPASLPILAIAANLTAPSVPANVYAVGQNLVPPASISVSWSANPEAD
                     FAEYWVFRSVTTPAPYVGTPVAIVPRGTTSFNDTTVAVGTRYYYRVIAVDKGGLKSPG
                     SQSAEVSDVALPPATLAAPGNNATTASLTPEFRWVAVPQAVGYKLIIYADAGGFTQHG
                     TAYANSPATSIIYAGTPLISGRTYYWRLAATTKGPDQINSISTMWRFTAP"
     gene            6906..8024
                     /locus_tag="PLT86_00045"
     CDS             6906..8024
                     /locus_tag="PLT86_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35037.1"
                     /translation="MTSKERILRALHREKPDRLPVSVHQWQGYHLQTYMGGITDIEAF
                     AATGMDAQIQYFQSMGQFWLVDADFSKTSTPQWRDDVQVLSNDPDHRKARHVITTPEG
                     ILTYETEGDRKTTWITKYLIEHDEDIELIEKYMPVAVFDPAPVAQLYEEIGDRGIIRG
                     FVWGDQAGCWQHACCLEDVNKLILACFDKPDWVHRLLRSLLEKKLQFIAELKGARFDL
                     IETGGGAASSTVISPDLHREFCLPYDRALHDALHDLGFLVTYHTCGGTRGIEELIVEN
                     GCDVSETLAPRSIGGNQEPWEFAEKIRNRVALIGGMDQYNCLTTGTPQSIRQHVHTLF
                     ERVGRNGGYVLSTADHFFDTPVENLNAYAAAARECTYS"
     gene            8065..9150
                     /locus_tag="PLT86_00050"
     CDS             8065..9150
                     /locus_tag="PLT86_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35038.1"
                     /translation="MTHRERFNRIFRYQPVDRAPYWGTGGWSETMRRWVSEGMPEGVS
                     LSKYFGAEETKGIPVYYGPWPHFPYEVLKEEGATQVVRNHEGIIMRVYKTDGDRSMPH
                     FLEFPVKTRDDYRTIIKPRLCQNPRDRFPADWNRRCAEWKERTDPLCLFGDRWSGFFG
                     PLRNLMGLENLSYAFYDDPALIEEMMDDICDNILLITEQILNDTDIDYWAFWEDMGMK
                     TGPLLSPELFDRFMVPRYRRVTDYLRSRGVDLIFVDSDGDVHALIPLWLKAGVNGLWP
                     CEIAANMEPAPIKNQYGRDLLLCGGIDKRALAAGPKAIDEELKKVPPLVVGGGYVPMV
                     DHSCPPDISWSNYCYYMEKLKEAIRIA"
     gene            9429..9719
                     /locus_tag="PLT86_00055"
     CDS             9429..9719
                     /locus_tag="PLT86_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT35039.1"
                     /translation="MTTTKRDLAKYVAKDTGCKQSLAAKMVDSVFEGMRESLMRGDRI
                     EIRGFGVFEVRNTRPKPAARNPRTGDIIQVPARRKTHFKPGKILKEELRRPR"
     assembly_gap    9812..9911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9912..10997
                     /locus_tag="PLT86_00060"
     CDS             <9912..10997
                     /locus_tag="PLT86_00060"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HOT35040.1"
                     /translation="LTLGGTSANMAVTVASLGVPRVLVYANPLTKPLAQAFPSLPNLL
                     TIGPDGTVGSPRDVAQGDDVFAIHWIFEYQKDDTIRIDGQTLTAPRANRFIPSWNPAN
                     NQLRLAPGFKATFLKDADRFSHLLVSGFHILSDRYPDGTTAQECIVPVADYLRLVRTR
                     APHMRVHCELASIAGKIVRKGVLDHILPQMDSIGLNEVELATWLEEIGAGELAGNVRD
                     QNAPEAILAGLNRLADATGVPRIHLHNFGYYMVLAEKTAGSPEGIRQGLACGACAAVV
                     RAKTGRPPKSDEVTSFVDLPLRAEAFDAMRTLAPSAKTPDFALTGVGTSGGRNLVFVP
                     TRIVERPARTVGLGDTISASAWLAEGD"
     gene            complement(10994..12142)
                     /locus_tag="PLT86_00065"
     CDS             complement(10994..12142)
                     /locus_tag="PLT86_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT35041.1"
                     /translation="MRFVLLYFTHFISFGAFVPFLPLYLRRAAGLSHVEIGWVLAVSN
                     LVSLVGLFVWGPFVDRTDKRRRTLVLGMIASAALYPAYMLTRGLPEIIGLTIVLGVFM
                     TPVIPLLDDLLLTHIRIHGGEYGRTRLWGSAAFIVGSTAAGWLLGHGVAGGQFFVFIA
                     AEIAALVVLATFSSEEINRPGAAVKDRHFWRSLTGPFIVFLVAAFLGRISAIGHYNLY
                     SLYLDKIGVSDTLKGVAWSIGVVAEIGMMFFTGRIIRRFGARWLFTLGLVGSAARFAL
                     YAVWPTPTGAIAGQVFHAFTFGAYHVGAVTLVTELAPPGRSATGQFALASLAYGLGST
                     VGSVVSGQVAQIWGYQVMYVMCAGLAVLAALVAQISAPEKGRTVFSAP"
     gene            complement(12247..15543)
                     /locus_tag="PLT86_00070"
     CDS             complement(12247..15543)
                     /locus_tag="PLT86_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT35042.1"
                     /translation="MSALFDQDARDRAQRSTNETLLVEASAGTGKTTTMLGRLLFLLT
                     TPTSEGERPPRLTEIAAITFTERAAQELKDRLRARLFATVSSVSPAQRGLVQQALNDL
                     EEAPIGTIHGFCARILRQYPIEAGVDVEFRVAEERAAAEIWRDVWRSWITTQFHRPDL
                     PEAVRRSVELGLTPDDLYSVAEAIRRFRMHPVSEMLGPETAAAPDEGAIEHLRELLPA
                     SNRDSRGRMLPGKRLATIETVRAFVEALSAATTQEERVNRLLQPLPESSAAELAPLLT
                     YVDELRTRYTHNLISGIVRWLTHPEEGIIAAYARAKHDAACLDFDDLLIKARDLVRNR
                     SEVRRQLQEQFRTIIVDEFQDTDPVQAELIILLSQRISPVDDGPAPPWEESGLSPGRL
                     CVVGDPKQSIYRFRGADIETYSRVASLIGVENRCHLLTNFRSHGSIIRAVNAVFGRPG
                     VFSSPDLPDEARMFIPAYQPLEPPAGMQEDAEPRVLWVEPATERCDALKDARPVEAAL
                     VANAIQQVVADGWEVRDPRTGQLRPARWGDVALLCRRWAVTEELEDEFRYRRIPYRIL
                     GGKGLFEREEVVRLVSLLHALEQPHDSLSVIAALRSAFFGVSDAELALAAIADASNFD
                     YTDDRQDALPETIGRAFALLREARASLNELRPSEVIEFLFEKTGVLAAYAQQPDGEQS
                     IANLLKIVDEARRYEAENSYSFRGFCNRLRTLRLLNAEESEAELDDEAGGRVQLLTIH
                     KAKGLEFPVVFLLDLGAPLNSSQSSEKLVMVRGDSRTPPRCGLLVKKLPGGASFCTRD
                     WKEVREADRERESAEMTRLLYVALTRARDYLLLPRVLVTKNATWQSLIESASESHEWP
                     VLQPRDFRDSQSAAPAVERADVISAFETELRRFEEKRRDATEQASRPRVRIIYPSSEH
                     PDERERWADESPTPAVHGSPEDDSGRRLGTVVHRALQQLHPGDLSDIERVVKIEAEEE
                     DLNDEERARAVALVRGCFSLPVINRAYSAPQRWKEVPVTLCEDTNGVRNIIRGVCDLV
                     FTQGSELVLVDYKTDRGSPQDADNLKLKYKPQLDAYVNALNVATGLTVAEAWLCFLGL
                     DGGPIECRVV"
     gene            complement(15540..18911)
                     /locus_tag="PLT86_00075"
     CDS             complement(15540..18911)
                     /locus_tag="PLT86_00075"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT35043.1"
                     /translation="MHEIVTGPPHALFSRLARTILEFRKPEKFARITVLTPDRQTRDV
                     VRDALPVFLAQEVPSAEPARAGLFNVRIAHLAPWAGEIARHWIEDRGLRPLPRSASLA
                     VISEAVRRTTHQTNAFAVEGAAFDPRDRPSFLRAVLREIVELKKACVSPSDLQERTGA
                     RYRKEIAAIYHASEAILQSNKWVDQSDILALATKRLKTDPAAPEEDFYLLFGFLHLNE
                     LEKRCLGALAAAASRACAYVPSVPGSVAFAVAEPTIELFEAAWGIARCSIPAKSGVAR
                     EIVGDLFCSDAEEVHENPAICVASASEPAQEIREAARWIWKVLEEDGLRFQDFQVIVP
                     DKRHSERLAADVIRKAGIPVNMVFGEPLCDTDAGRGLLLFLDAVESGLLRTRVMDLLS
                     VCKPRATNPPGEQARSVNSSDWDRIARIAGVFGGIRPDRDVRSEWNDRLARYERALAW
                     RIEHQPAEGEEDAVEDVASLRREHDTTCELRQLIHELDASRDAIENASSWEGWVAAVK
                     QGWQQTAEVGSSEEVRQIERVLDEIATYDAAGKLPPAPVRRTILEEALGHTRASTTHS
                     DPHGVVLTNLTGACIRPCRALVMVGLSDGEFPRTTTRTALSVQPDDGLRHESASLSLD
                     LQKAIDYAMFAMALSAASDRVRLSYSRRTDAAAAEKLPSSALMEVIRRVIPLEESPSE
                     TPLWDEKALHRELSERGEWAVREPVPRPKAFWLNAQELILAHAAGVAGTSAGEQIAGR
                     LSRHARNGMALLRARDSFPGPYDGWFAERSVDDTLVNHVSSLGTGHNPVSTRALEAYA
                     ECPFRFLVTRILKTEPLPEPDIVLDFSGMDFGLVLHELLAEFFRRLINEQLFPRRPES
                     ARDLRPLFDELAEEKRRELEREGTVPPGVIWRIRWTALQESAWAAVAWSLGDVHSWEP
                     SLVELGFGVSGGRVDATSPEDPAFLPLADGGTLAVRGIIDRVDFSREAPCARVLDYKS
                     GRKPRTLNGLTNSGQLLQALLYARMLDQWLQEKALAREVSEAWYLYLKNPVTTDDVDS
                     VPVFDTLSFSGETLHNGQEELDRVLGVIIPCMRSGFFPPLPQDPPNGEHGTCAHCDVA
                     VACGSLSGLTSRWKRYRKDLRSACFLSLRSKGGTP"
     gene            19134..21014
                     /locus_tag="PLT86_00080"
     CDS             19134..21014
                     /locus_tag="PLT86_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35044.1"
                     /translation="MLEDAPLEHELHNLGNDCYRSGQFEKAVEFYCRAIEENGRMVET
                     FFNRALAYARLGEYDAALNDATYVIEQNASLHDAYYLRGRIHELRLDDRAALADYDRA
                     ISLNPDFHAAVEQRTTVVSREWTYRDLRNLRAQIAIEPGNAYLCFQFGQRLLLIGHVD
                     DAIRYLETARELGFAFGDLWGELGNAYRQSRQPVKAIAAFRQAIRVNPTDVASCAHLG
                     QLLTESGHYRDAVQVFRRALGMPGDDVAALFCGLGAALYGLRKWEEAREAFQRALDTE
                     PRLPDAAAGMGLIAWRLGDRARAAQWCRAALELSPGATDAIVLSVNLALSEGALATAM
                     EMLYELFQANSEDHRLAYVLWTVDTYARKQPGFLPDEGSAVWARRRKTRETARRVVRS
                     ALKHRNDLSFVQRDLCDELLASCPVCEEAAEALYQKLITAGNDVLAVLYREIRQPALV
                     PSAERLRGAACVARMQDERWLVDVCLAYADVLVGTPEAPRVEGLARVLTRLSVRGVDH
                     AEGVRDLYRFCLEGTRAGTVHTMIGLSPRVFRLALTINKPPSILPETGEFLIRLSEWL
                     ERFERAARWVLPAREWPEVGDELAELRNNLIESVPAPEGWALASVLTNFGSIARHYVP
                     IN"
     gene            complement(21064..22062)
                     /locus_tag="PLT86_00085"
     CDS             complement(21064..22062)
                     /locus_tag="PLT86_00085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT35045.1"
                     /translation="MPEATHTLTQFEYPVLVVGAAKLANDLRAAFESPSTKAQLRRAV
                     YVDHAPAESGESLANKIADCIDPRYQAVFLDATDPVLVDDRVLTITEIKKYCPATEIV
                     VVIGKKTEGRLVELLQAGAWYFLQTPITEASLTTIMVRVVAVREADALVRTDGLTGLF
                     NRAFFEDALRDTVARLRLDIGGQRTATAPPVSLLLADLDGLKEAYGDRHQEGEMFLRE
                     MGGVVRKRYRPTDVIARIGGDEFGALLVGMNYTLALMRAELLRKETLKIPLPEGIATR
                     PTLSVGVVTYPSYFDDPQQMYWQARRALERAKAAGGNVVFGFDATGTPRPFVELGG"
     gene            complement(22099..22926)
                     /locus_tag="PLT86_00090"
     CDS             complement(22099..22926)
                     /locus_tag="PLT86_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35046.1"
                     /translation="MRFAICNEMFQNWEVNDVFRLAAEIGYDGVELAPFTLADSVRDL
                     SPERRREIRQQAERAGISIVGLHWLLVSPKGLYITHPDEAIRKETAAYFVDLIHCCAD
                     LGGNRMILGSPKQRNLMEGVSHERGWQWTLDFLRSIVPVAEAREVTLCFEPLAPAETN
                     FIQTAEEAIRLVQGINSPNLKIILDVKAMSGENKPIPEIIRASGPWVAHFHANDANLR
                     GPGFGDVDYAPIVAALRDIGYDGWVSVEVFDFSIDPEVTARRSLSYLKEVFGIASSH"
     gene            complement(22939..23493)
                     /locus_tag="PLT86_00095"
     CDS             complement(22939..23493)
                     /locus_tag="PLT86_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013680234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT35047.1"
                     /translation="MERGVAADGIGLVGVLADSHDNRWALEAILRRFADEGVVHVLHA
                     GDFVAPFNARCLSGISVPFTGVFGNNDGERPGLTKAFAKYGTLHVGPYAFDTRGRRII
                     LMHEPVAVDALAVSGRFDVVVYGHTHETDVRKVAWADGNGSTLILNPGEAGGWLNGRA
                     TAALLDLADLNVNVFDVPFARTSE"
     gene            23696..25222
                     /locus_tag="PLT86_00100"
     CDS             23696..25222
                     /locus_tag="PLT86_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35048.1"
                     /translation="MEHWHRRCAVRPALLVAIVVATVPAWLAAGDDYPPIQITDLQTP
                     VERATFVVQTLFDSTNGIEDVYHAAWTVAGKLYYRSGVITSLARPRQLVTGLQACAEP
                     LLFMLPSGKPGLVWAEERTPNLSIVPGEWNLRYTADVNVAPRWFTEDAGIGGVHPDRN
                     PSVAVQGDTVLVAWERDEPPQVAYAFLSPRDIFPLVRLLTSYPYGVSRPTVAADTEGA
                     YWIAWTAETPTGGDVWVARVSRNGATSASPVRAGPEDARNCVIAVNADRETYVAWQEG
                     LSGGSIYLVRALANGRFTPAVKVSPEGTVCATPQLCFESGTIPCVSWRNVLTGDLEMV
                     RVHGTRPMGPVNLVSGVYTPRQEFVTPAQLRSRSGCVVSAFLGPDPVRGVTNLWISRG
                     KEYTPGADSDSNGGNKPAAPSPFFTRVSPNPFNGSVLLEAHIPASVAWRLEIRSIDGR
                     LVRKFEGSSVAPGPVRVVWDGGDSFGQTLGSGTYLVRWSVGLPNGEMWTHTERVTLVR
                     "
     gene            complement(25206..>26081)
                     /locus_tag="PLT86_00105"
     CDS             complement(25206..>26081)
                     /locus_tag="PLT86_00105"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT35049.1"
                     /translation="PGEEVSCGNAEGRNVHLLALGIHEHVPGKGDSAERPFRNRPDHS
                     ITEVIDLVQRQGGLALAAHPGFTPPRIQRLVFRRGVWKPDDLAREGLTGIQAGGRFSD
                     AGFRRGMGQWVRLLLAGKNLCVVAGNDSHGAFGRTRQVKIPWWSIAEDSSYVFGDVRT
                     VVRADSLSNHHLLDSIAAGRCYITNGPAIRLDVSRGTTRAGMGEELAGGNVTVHVEAE
                     STREWGYVDRIGIAVGQTGGVESMTWKKPENPALRFGVDVDVPALSEGYVRVEAHTDM
                     RRHAYSNPVWITAPA"
     assembly_gap    26082..26181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26182..26625)
                     /locus_tag="PLT86_00110"
     CDS             complement(<26182..26625)
                     /locus_tag="PLT86_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35050.1"
                     /translation="MLLYAETHYPRYWLSRIRRAEPEIVVDIPWRVEPGAGIPVLLLV
                     HHANRYPVLLESVSVTVNAGTSGCTTAEFPLHTAISTQYWWKLLTLPPLAAGTTAEIV
                     PVIRYSVKGRSREMVADNYRGTSHQPFRVHIAEEPYPLPAGWYAGD"
     gene            complement(26640..27296)
                     /locus_tag="PLT86_00115"
     CDS             complement(26640..27296)
                     /locus_tag="PLT86_00115"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT35051.1"
                     /translation="MQLGRRLWSSKSIRRALLGLLVGAAVCSLHPDRAVLIPFLFWSV
                     PTHSMLMVPNEPAAIAAVQMLIRKTSLYDAALLVALTAAIGAGVACVIDQTVLQWFAR
                     KDRLRGFLNHSFTGRAVEWFRVWPFGTVFAFALLPLPFFLVRFLAAAAQYPIERYTAA
                     TMLGRIPRIFLLCVIGGFWVMPPWVVVALVVAALGTASVVPRRLVRIRSTVSQSVETD
                     "
     gene            complement(27308..28162)
                     /locus_tag="PLT86_00120"
     CDS             complement(27308..28162)
                     /locus_tag="PLT86_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOT35052.1"
                     /translation="MAGSPERGSLVSYHGGHTLYDGTGRLEDFVQAAVRQGFAGLGFS
                     EHMPAPPRYPYPDQPSWDEAVRKFDRYVDTVQELKVTYRDELPLLLGIEVEYLPDEEE
                     YLTDFLARYPFDYVVGSVHYVAGIGFDHCREWYDEAAQKCGGYENLAVSYYTNVRGLL
                     ALGVTDVLGHLDLINIFSPAPISGDDVAEAEEATLAAALQWNVILDVNGRGLIKPCKQ
                     IYPRAELLRKAREMGIPATFGDDSHAPDQVGARLDEVRASMRVAGYQTVTALLPDGHQ
                     IQRVELPL"
     gene            28317..31400
                     /locus_tag="PLT86_00125"
     CDS             28317..31400
                     /locus_tag="PLT86_00125"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOT35053.1"
                     /translation="MIHAQSTFAAILAVTSAVTSVIALYAWLQRKTPGAACFAGLMTS
                     ITVWTFTGMAEMLTASAAGKILWSKASYLGILTIPPLWVTFALTFSRFERWLTRRVQA
                     VIWGVPAALLLLVFTNEFHGLVWPSIVPLTDEPGSVLIYQHGLAIWLVMAFSYTGVVF
                     GCIWLFYAAISSPRLRKGQTVALVIAALIPVASNFVYMADLSPYPGFDLTPLSFAVSG
                     AVLAWGIFGVRLLDVVPVAHHALFSGVTDGVLVIDLEERIVGVNPAATRMFGLQSSPL
                     GKSILEATTHVTGLEFRFENGQREHGEVTFRRGEDQCWADVRVSPLVDGRERKIGELI
                     ILRDVTARKQTEERLRLAQEATAAANRELTAAIAQAREMAERAERANAAKSEFLANMS
                     HEIRTPMNGVFGMTQLLLDTDLTREQRSYAEMLRSSAESLLSVIDEVLDFSKIEAGKL
                     ALEITEMNLRTVCEDVSAVAAVHAQAKGLEFVCDVAPDMCENVRGDPTRVRQILNNLV
                     GNAVKFTAQGEIVLSARIESQMKKALNVRFSVFDTGIGIPKEKIGMLFNPFTQADMST
                     TRRFGGTGLGLSISKRLAEMMGGQIGVASEEGKGSRFWFTAQFIRPEAVEERSPDTHA
                     QLAGRRILLVDDNLSARRAASSLIRAAGCVCEEAGSLAEARDIFAAARQNAEPYHAVL
                     LDVPLDFGDPRAAIPRIDHGFDGTPIVLLVPLDKRGRAAQVEFEGVAASVTKPFRRDD
                     LLRCLTSVTGSFNSAHPPVPRFVPPSTAKHYARVLVAEDNPLNQKLTQIMLEKLGCEV
                     VTVSNGVEVLSELASKSFDLILMDCQMPEMDGYETSRRIRESTVPRIREIPIVALTAS
                     ALIGDRDRCIEAGMNDFLPKPLQKSDLVATISRWASGDRSGANGRSGTGEVLAFDPEK
                     LMGRVGGNFHAARQLVEAFLRDARDHVALLGNAVSEGNASLLAAEAHAVKDNALHTCA
                     PALASAAAALEAAGTQADFARYAELIEAVQRHHADAEREMCVFIGSEPKTEQTLI"
     gene            complement(31474..32877)
                     /locus_tag="PLT86_00130"
     CDS             complement(31474..32877)
                     /locus_tag="PLT86_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT35054.1"
                     /translation="MNLVLVILDTFRKDHVGAYGNPWIHTPHLDAIARESVLFTEAYP
                     ESLPTLPVRRALHTGQRVFPFHGHRPLKGDFVGAPGWGPINEEQDTLSELLSEQGYRT
                     AFITDTYHQFKPSKNFHRGFDEWVWIRGQESDKYLSGPPVLEAEIMARSTPSQKNDVW
                     FHRFMTSYLRNNTYRMTEKDYYPARLFSEASRWLWDNQDAERFFLTVDSFDPHEPWDP
                     PDHYRKLYDPDDDCANVIQSPYRRWKERLTERELRRIQANYAGECTLVDRWFGFFMET
                     LRTTGRLEDTLVVIVSDHGHNVGHDPGDKGLVSKQGHPMTKAVAQLVCMIRHPKGEGA
                     GTTCDKLIYNMDLSATMLALAGVEPKQKPDGINVWDAIRDTKKPLREYVTVAWGPLVT
                     VIDDTWWYNANIWGEGALLYRHREDPNLVTNLAEKEPAVCAMMRERAVQDAGGSIPEA
                     FKNYATIPGCTPYLGKD"
     gene            complement(33009..33938)
                     /locus_tag="PLT86_00135"
     CDS             complement(33009..33938)
                     /locus_tag="PLT86_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT35055.1"
                     /translation="MNGTASQSPADRDAEIRKLRTLLRVSKIMNAESNRAKVIQRINE
                     EVREYVEADRFTVFFHDSDTDELYSYIASGLTYGEIRIPSTAGIAGAVFTSRETLRLA
                     DAYEDPRFNREIDKRTGYRTRSLLSMPIINQRGTCIGVVQALNKKTGNKEFSDEDVEF
                     LKELVEQISDLLDLLLHKEELTRRQAALQESLSRLAVYDYLLGEKTAMKVAMRWNRKL
                     HLWIGVAGIIGLTLMAITGIVVAHVRGRPYVTMLDLHTGKAFLPRELAFTYSDLTGVF
                     LAIVTLTGVVLWLYPVVTKWARRRLERKASARD"
     gene            complement(34004..34414)
                     /locus_tag="PLT86_00140"
     CDS             complement(34004..34414)
                     /locus_tag="PLT86_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35056.1"
                     /translation="MRTGAIVAIIASMGCALIACGNNNAEREAKLAAMSAQEREIQKV
                     LDQKCVKCHTAPDAKGNLDLTEPKNLLPLISSTQLFDDIALYNMLMGDSTIAEHAPKE
                     FQLQQSEVDMIRRWVLSEHSDLVPDSVRAQQQGT"
     gene            complement(34539..35660)
                     /locus_tag="PLT86_00145"
     CDS             complement(34539..35660)
                     /locus_tag="PLT86_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35057.1"
                     /translation="MPMTSHERLMAAYRKQVPDRVPVRVWAVDDRTQPIHPSYAPIVE
                     AAREKTDLVGGWGGGPRREMLCRVTHEERPSRIAEFEERVTTIHTPAGPLTRVDVFNR
                     SGKPGYCMKHLIESPEDAKRWLSIPFGPLQGEVSGFFERVREMGDRGVIMSGVSHPMY
                     AVQDRLGPEGFAFWSIDERPLLHEMIQRELEMTLEYLRYILERGVGPLFAYVGPEVCI
                     PPSQSVRDFEDFVVHYDRQWIELIHEFGGIVWCHCHGNTKAVLEGFIRAGNDVLQPLE
                     PPPWGDIELRDIKRQVGRRMALEGNLEKHEMYTSTPSEIRERVRQAILDAGEGGGFVL
                     CPSAGLQEWPTCDEQTVANFLAYIDAGREYGKYPLSREW"
     gene            complement(35748..35873)
                     /locus_tag="PLT86_00150"
     CDS             complement(35748..35873)
                     /locus_tag="PLT86_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35058.1"
                     /translation="MKKLLLLLALVGAAVALVVWYRQTSASLDMVPEDAEDIELA"
     gene            36129..37220
                     /locus_tag="PLT86_00155"
     CDS             36129..37220
                     /locus_tag="PLT86_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35059.1"
                     /translation="MSADIRGEWPNYGGALDPTVWMRIRASMNHEEGDRVPIWDYLDN
                     RPTFEYFHRPGDTHDETMRRAYHELGIDFCRGYGSSFSREDEGRASDHEEWKISGLTQ
                     WATKKPISNYAELRSFQPRLFTEVDCSRWLKQTQEAQARMAPLSYFVPGVGTGFHEVY
                     DLMGQQLFSYAIYDERRELDRLFEAYGYNSTLIARHAASTKPGPLFFTWADIAYKGRL
                     LFSRSFLRETFIPMLRSVCEPLAEAGIKVVFHSDGDVTEILDDMLDAGINGLNPVEPQ
                     AGMDIGRLKKRYGKRLILVGNIDCSQVLPLGSVQDVVEAVKTCLREAGHGGGLFIGSS
                     SEIVPSTPLENVLAFFEACRTFGRYPLAV"
     gene            37771..40884
                     /locus_tag="PLT86_00160"
     CDS             37771..40884
                     /locus_tag="PLT86_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT35060.1"
                     /translation="MQRQRYVWLAGLVVSLMILVAGTAHAVTAGKIIGKVTDAQTNEP
                     VLGAAVTVEGLRMGATTDADGRYFILNVPAGRYTLKVQMIGYAPVTQLDVLVNNDLTT
                     RADFRLRYQAVEVGEVTVVAERPLIEKTLTGTRRTTLREQLELMPVITSDDIYRAAPG
                     IVFDPIGGPVQQVTDGTIRVEDETRSGNTANPGYYIRGSRPGEVNYYLDNFPVSGVLA
                     LPGKAIEETNLLTGGFSAQYGNGTSIINTVTPTPGAAYTASLNWESDLLPGLVSERYN
                     YGTNVVTASFGGAVPGTNNRLRVWVLGDGNMTDDWDPRLSTQDANFIENGIREFTQRA
                     NMVDTLRTFWATLKPWDVFEGDRLILPSHKQDMYTFMGKAMYNVGAFQFTLSGFAWRR
                     QYQRFSPNGLFMDWDKNNFRNMPYPTTMQQATLLHRNLVAFGVRWALSPRTIVDAKLS
                     FSTTATTTGSLDLKSGSFGLFNDFDYSWHEYAPSWSPFYVDPADWATTNRAKYTQQRR
                     EVYYPLGVTNLFFTSGNSRSANAVAGNNVVGQLHATSQINNNHELSGGVEITRSMTKQ
                     DRLSDPWSGTPFFDQWGPFNPIYGAAFLQDKIEYEGLTVTAGVRMDYYDPDAYVWSDP
                     LKPGAGSIMPDGSINVNGRPDVNADKVNAFRDKRVDPKMTLSPRLGISHPVSDVGLVY
                     FNYGHFVTQPANPYESYVPDLNRSNSRLGNPDLPFMRTISYEIGYSHELVTNTKLDVV
                     LYRRDIRNTNTYQRIPAMNGAYTYAMASATTLVNGKEVNIGYGNSKGLELILTRYRGA
                     EGMFSGWLSYSYLKAEAIYSDANDAYERFSRSALDPETGELAYPPDVPGIADYDRTHS
                     FKANFDIRFRQGFGPQVAGMFPLQNMGINILEVINSGLPYTRINMSGKPVGINNGFRK
                     PWTYETDLMLNKQLRLANVPVTLYAKVENLFDTRNVLNIYERTGNPIDDGRIMPQAQP
                     NSVTPASPTWVYEGVKDGIDGSTPDGRISIAEDETAYRKAWSIYARDPLFFSNPRIIR
                     IGVGMSF"
     gene            40937..>42359
                     /locus_tag="PLT86_00165"
     CDS             40937..>42359
                     /locus_tag="PLT86_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35061.1"
                     /translation="MRFSPDRLNARRFPWIGIMVAVLVVAGIASAIPARNAARVARSG
                     RIASDHVSLEMRWLDINNFKCPITNYGFIGNNNAENRAGGEWPAGSGEYYLYGGGIWI
                     AGTVDQGPFTENIGGNTVYTFRPSTTNPLGWQGLGAGGRAILTDPRAVIGYEPSGGTA
                     EMAPLTKVFMSTESDWPFPNTVSILDTYTEYDDQDPQRWNTGTGSNSGKDTGKWSGTP
                     TASTIATYGLGVKVKQTTYSWNYANNSDIHFVVYDVENIRTDGKTINNCFVGVVSDPD
                     IGNEAADDMAGFDASRNLGYAYDSDFSEAEFRGVPGFIGYRFLKSPVAEHDIDKNGDG
                     VIDNNPVLINSVPVYDVRAGQQIGLHAYKIFGRIAGDPDTEWEWYMTMAGHNFREDQN
                     QVYQPFDLSNTPEDQRFLQSTGPFTLKPGQPVQIVVAMMVAKAAGNPGDPISVRVAEL
                     QRVSDVAQSIFDNNFLLPVPPKAP"
     assembly_gap    42360..42459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42460..>43868
                     /locus_tag="PLT86_00170"
     CDS             <42460..>43868
                     /locus_tag="PLT86_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35062.1"
                     /translation="AQSIFDNNFLLPVPPKAPALTARAGDRTAYLSWDTVAETTPDPF
                     YSVASDPASALYDPNYKRYDFEGYRVWKSLTGRPDDWELLAEYDRQSVVPRNAEITVQ
                     AGVYGGTLTYDAATTEGYFLEYGGTSLFNGHEFLVMLDGATQKMKVFDVSLGGQEIPC
                     NTYADGDDWWYYHGDNSQFELFDSSLNYLSSDSTRNANGINDQYVPGMIMYLAGMALQ
                     FDNVPAPASTVIWRIVPSQEEDFGANTTVAHSYIDRDLINGQTYYYAVTAYDFQLSSP
                     RSLESGRTLSQAVVVPRSEPNGLATPDVAPVTRISGSSDGVVNLEVVDPVSVTGHTYR
                     IGFKADETWFVRDLSKPSGADTVVNNVANQAGGFDYPIVDGVLVKVIGPSIGIESSRY
                     VPSANKWFVERAAGYEVMTGVDWGWGTTVTGADYARIEVRFNGGSTRAPVYVRGASPN
                     YSYQGAGTIPAEIWDVTNNR"
BASE COUNT         8914 a        12824 c        13073 g         8757 t
ORIGIN      
        1 gcggaacgct ggggtgcggt ggcagatcaa ctcggggcag agaaccgttt caatcaggca
       61 tgggcacttg tccagcagaa gctccgtgag ttcccggaaa acccgcatct gctgtatctt
      121 gccgggttct actacaaccg cgcgggaaac aagcggtccg cactggaagt tctcgaccgt
      181 gcggtgctga cggatgaccc cacgtggcgt ggcctttcat tgaacctgca cgatagtatc
      241 gaacccccgt tatcagaaga cgagatcaac caggccaaat ttttcggccg aaaagtcccg
      301 cgaattcatc gttgcagaat tccgggcaga gaaaaacaga acgaagtaat gcagtaagtg
      361 tgaccgaacc gcgtgaaaag cgggcggcag agtagttcgg tgagcgtgcg ctgtacggcg
      421 ggtccgtatg ggctacgcta taccatgcaa acccctcgga ggcttcagcc cgaggtgagg
      481 acaatcagga ggaacagatc gatgggtggt cgtaaggttc tcaccattgc cgccgtcagc
      541 gcattgctgt tcgggatggc aagcgcccag acaggagccc cggcacaggg cggtgcagcg
      601 caatcggccg atacggcgaa agtccaggta tccttcgtcg ttacgcagga tggtaaggaa
      661 acggcgatac tcgagaaagg gaagcttgtc gaggtcgtcg caacggtgaa gttgaacctg
      721 ccggacgcca agaatgcgaa cgttcaggtt attgccgaca tcgcagatca gttgaacatc
      781 gaaaacgaag ccgacgtgaa ggtgaagggc ctgtacattg acaatggcag cgggttttcc
      841 cttcaaccga cgtcgtctgt gcgtccccgg ctcggattcc ttgcgcccga aggtcagaca
      901 ggtatttccg cgacccagcg ggtgaaatgg gtgctggatt tcatgaatgt cgcgccgcgt
      961 gcgccctttt ccgtgaatgc ggcagtcgta attcctccca gtaacgaaat catcggtcag
     1021 gcccgcgccg agcagatcct gaaagtcgtc agttcgctca cggtgcagca actgtggatt
     1081 gacggcggta tcttcatgtg gccgctgggg ttctgcctga taatcggaat agcgttcgca
     1141 ctcgagcgca tgttcaccct cacgttcgca cagatcaaca cggagaaatt cttcactcag
     1201 ctcgcgacag cgatgaaaga tggcggggtg gagaaggcca tggaggtctg caatgctacc
     1261 cgcggccccg tggcggcagt tctgcgcgaa ggtctcctgc gcgtgaagca gggcatagac
     1321 cacgtggaga aagccattgc cagcgccggc gcggtggagg cgagtttcct gcagcgcgga
     1381 atgattgtta tcgcatctgt taccgtcatt gccccgttct tcgggttcct tggcaccgtg
     1441 tggggtatgg tgatcgcgtt caaagctatt gctgaagcgg gcgatgtgaa cccgacaatc
     1501 gtcgccagtg gtatttcgca ggcacttatc acgacggtcg gtgggttgat tatcgctatc
     1561 atttcgcagt ctctgtacaa cttcctcctt tcccgtatca accgtatcgt aattgatatg
     1621 gaagagacgt cgaccaagct ggtcgatctt ctcatcgagg aaggcatgga caaaccggcc
     1681 agttcctgac agtcgaccgc gaaggatatg aaccatgtta atgggtaagg gcaatctggc
     1741 tccttccgtg gaaatcccca cggcgtcgat ggcggacata tcattccttc ttctggtctt
     1801 tttcatcgtc agcacgtcga tgaaccagga ccggggaata ggaatgacgt tgcccccgcc
     1861 gggcaacccc atgccgattc ccaaggaaaa catcgcgagc gtgtggatca acaaccgcgg
     1921 cgaggtggcc ctgaaatacc tcgggcagga ttacattacc gtcacgttgc cggagatgcg
     1981 cgagcggatt gaacgacgga tcatagagaa cccgaacctt gtcgtatcgt tgctcgctca
     2041 gcgggacgcg gactacaaaa cgttcatcga cgttctcgat gaactccgca tggtaggaag
     2101 tcggcacgac tggtcgaaga tctcaattgc acaacccttc ggatcagccg gtgtcggcgg
     2161 aggcccgggc ggccaataag gttcgggtgg aacagagcca cgacgtgagg agtcgtatac
     2221 atggcgcttg taggtggcaa attcaagggt cccagccagc cggaggtggt tattccgacg
     2281 gcatcgatgg cggacatatc gtttttgctg ttgatcttct tcatcgtatc aacggtgttc
     2341 gtcaggttcc gcccggagtt tcagcctcag ttgccgaaag cggagtcggt cgacgcactg
     2401 aaaaaccggc gaaacatatc gtatttgtgg atttcgccgg acgggatcat tcagattgac
     2461 agctctcttg tgaatctgaa ctcggtcgcc ggtgtcatgt tgaagaagat ccagaagaat
     2521 ccgcaactga tagtgctgat caagagcgac gagaagacac ggtacggcat ggtgtcgaac
     2581 gtgatagaag agcttcgcaa ggcaaatgcg atcagaattg cgtttgccgc gaaatcagaa
     2641 ctcgaatgac ggaggggata cggcatggtt ggcacctctg ttaaaccccg tcgcgacatc
     2701 gtcaaaacta tctaccggcc ggttctgctc gtttcgacac tcgtttctgc gtttctgtgc
     2761 gtggggttct acaacctaaa catgcgcatg gaaaaggagc ggatatcttc gcagaagcaa
     2821 gggcaggcga ttgaagtggt ggacattccg gcgacggctc agcaacagga acaggctgct
     2881 gctcccgctc gcccggccgt gcctgtggaa gccagggacg agagtcaggt ccaggacttg
     2941 acgatcatgg acaccgagct ttaccagcag gcgtcggttg cagtgccgga aaagctgcag
     3001 gaagcctcca acatcagcca gcaggtagag caggaagcgc cgcccatgga gttctggatg
     3061 gtggaggaaa agcctacgat tgtcacgcag gcgattcctg tctatccgga aatcgcgcgt
     3121 caggcaggga tggaaggcga tgtcatcgtg gagatggtta tcggtacgga tggcaaagtc
     3181 cagagcgtcc agattctccg tgggccaccc gttttccacg atgcggcgcg ggaagcggca
     3241 atgaaaatga ccttcacacc cgcgaagcag aatgaccgcc ctgtacgcgt gaaggtatcg
     3301 caacgcatca gtttccggtt gcgcggctga gaacgcagaa tcccggtttt ttgatcgctg
     3361 gttttaccct cacaccggtc tacctcgtac atttcggggt aggcactggt gtgagggttt
     3421 cttgttttcg cgatgcactc gcgcgggaca tgggtattct cgcgggaagg ctccggcgcg
     3481 cgaacgcggt ttgccagagg cggcctcgga tgaagtggtt tcacctgaca ctgctcgtgc
     3541 ttttcgcgtg ttctccgatc gtcgtaccgg aaaagccgac gattgtgaca caggcggtac
     3601 cggtatatcc ggagagcgcc cgccaggagg gaattgaggg cgatgtactg gtggagatgg
     3661 tcgtgggcgc cgacgggatg gtgcaggatg tacagatttt gagtggacca tcggtgttcc
     3721 acgagtctgc acgggaggcg gcggccagaa tggtgttctc gcctgcgagg caggatcacc
     3781 agagcgttcg ggtgaaggtt ctgcagcgtt tcagttatcg cttgagttcg caggcgctgg
     3841 cttctcggga ttccactgcg gtgccgtctc tgaacgaaga gattccgctt cttgagtatg
     3901 ccgaggttga agtgaagcct ttcccggttg gtccaccggt tccggagttc cctgagattg
     3961 cacggggcgt tggtttcgga ggagaggtcg ttcttgatat catcatcggc gcggacggga
     4021 acgtggagag tgcggtggtc cgtgaggggc cgcccgagtt ccggggtgct gcgcgtgagg
     4081 ccgcaaagaa gatggtgttc acaccgggaa tgcacggtaa ccgtcctgtt cgggtgaagg
     4141 cggtgcaacg gatccggttc cctttttctc ctcagtaacg gctcgccgga agccggaatg
     4201 ccggtcgggc gaagcctttg tcgttcgatt ccgcgtgcgg tacgccattt gcacaatcat
     4261 tctgcaggca gactgaaaag ctcggtttgc atgcggcgcg tgtttccacg ttatttttaa
     4321 cggttgcgtc cggtcccgcc ggttgtcaca cggcgttgag gaaatgatca aacggggagt
     4381 gttgttatga aacggatgtt ctacgctgcg gtgatgatcg cttgcagcgg cgttcttggt
     4441 tcgcctgtac gagtagctgc tcaagttacc gtcgtaaagc cggaactgac ggcggagcaa
     4501 gcactggcgc tgaacgcggt tcgcgattcg gcccgaacgt tgtttcagca gaggaagtac
     4561 cgggatgccg tggaggctta caagcgctcc cttctgatcg actcgacgga cgccgcgacg
     4621 tatttcaacc tcggtctcgg gtatcaggtg ctggggaatg acgacgaagc tcttgcgatg
     4681 ttgaatcgct ccgtcgcgtt ggattcgctt ttcgctcccg cgcaccttga ggtcggcaag
     4741 gtgtatctca aaaagcagga actggaacag gcggaagagg catatctcgc gaccaaaagg
     4801 atttttgccg attcccttca gtattccatg gctgctgacc aggggctgaa caacgttgct
     4861 atcgcgtaca gcaatcgtgc ggtggtgggg atgcgcgaga ggaacttcgg atcggcggaa
     4921 gcggacgcag agaaggcgat ttcgctttct ccggacatat cgcgcgggta cgtcgttgcg
     4981 gcaaccgtgt acgagaacca gcagaaatat gacgaagctg agcagcgcta tcgtgccgca
     5041 gtggatcgcg cggagaacga tcaggtgaag gcgaacgctc tcgaagggat cgcgcgatcg
     5101 atcctcgggc gcgtgagaga ttcccgcaac aacgcagccg gggttcgttc ggcgcgcgtc
     5161 tctgccgccg aaatcctgcg tcaggcggtg cagattgata cgatgaactt cactgcgctg
     5221 gtgaaccttg gcaacacgct gtttgatctg gaacagtacg cggctgcgga atccgctctc
     5281 gtgcgggcgg agagcctgcg tccgcgcgac tttcggccgc cgctcaagct tgccgaaacg
     5341 tacatgaaac tgaacaaatg tggggaggct gaacaggcgg ctacgcgcgc gctggcggtt
     5401 cagccgggga acgcctccgc ccatgcaacc cgtgcggaag cgctggagtg cctcgggcgc
     5461 actcgtgaag cgctggacgc gtacgttcag gccagccgcg atccgcggtg gaagcagtat
     5521 gctgatcgga aggcgaaaac gctgcgggaa caactaggtc tgccggcgca gaactgaagg
     5581 cgcttctggc tgaagcgatg cggggcggct gcctggaggc ggaccgcccc tttctttttg
     5641 cggcacgcgg tttgccggtt gtggttggtg caggggtgtt gcgggccggc gtccgctgcg
     5701 tatgtttgcg tacaagctgg aacgtcggct gcgccgttct cattggcgca gactgaatga
     5761 gaaggagcca cgtcgatgct ggtacgatgg ggatttctgt caggtgcgtg gatctggccg
     5821 gttctctttg cgatcggttg tggcaccacg ggcccggatc tggttgcgcc tgctacacct
     5881 gccgggttcg ggcgcatcgg cgggggcgac ggtgagaatc gcttcggctg gcagagaaac
     5941 cgggagaagg atctggcggg gtacagaatg taccgagccg agggtgaccc tgctgccgcg
     6001 taccagctaa tcgctactat ctctcgtgat agcacctcgt acaccgaccg caacctcgat
     6061 tacacgatcc agttctacta caaaatgacg gcatttgaca acgccggaaa cgagagccct
     6121 gcatcactgc cgatcctcgc gatcgccgcg aatctgaccg cgccgtcggt gcctgcaaat
     6181 gtgtacgccg taggacagaa tctcgtgccg cccgccagta tttccgtaag ctggtcggct
     6241 aaccctgaag ccgacttcgc cgaatactgg gtgttccgtt cggtcactac acctgcaccg
     6301 tacgtcggaa caccggtggc gatcgtgccg agaggaacga cgtcgttcaa cgacaccacc
     6361 gtagcggtcg gaacgcggta ctactatcgg gtgatcgcgg tggacaaagg cggcctgaag
     6421 tcccccggga gccagagcgc cgaagtgtcg gatgtagcac ttcctcctgc cacgctggcg
     6481 gctccgggca acaacgcgac aaccgcttcg ctgacacccg aattccgctg ggttgcggtg
     6541 ccccaggcag tggggtacaa gctgatcatc tatgccgacg cgggtgggtt tacgcagcat
     6601 ggaacggctt atgcaaatag cccggcgaca agtatcatct atgccggaac tccccttatc
     6661 agcgggcgga cgtattactg gcgccttgcc gcgaccacaa aggggccgga tcagatcaat
     6721 tccatcagca cgatgtggcg tttcaccgcg ccatgacagg agtctctccg aggcctgttc
     6781 tgtttggcgc ttgaccgatc gcgtcatttt gccccgacga tccgccgccg tactttccga
     6841 ttgtgccgtt gcacacctga gcctctgtgt tccggcgttc tctgcacccc gtggaggatg
     6901 gaaacgtgac aagcaaagaa cgtattctgc gggcactgca ccgggaaaaa cccgaccgtc
     6961 tccccgtgtc ggtgcaccag tggcaggggt accatctcca gacgtatatg ggcgggatta
     7021 cggacatcga agcgtttgcg gcgacaggca tggatgcgca gatccagtat ttccagtcca
     7081 tgggccagtt ctggctcgtg gatgccgact tttccaaaac ctcaacgccg caatggagag
     7141 acgatgttca ggttctgagc aacgacccgg accaccgcaa agcccggcac gtgatcacca
     7201 ctcccgaagg catattgacg tacgaaacgg aaggggaccg gaaaaccacc tggatcacca
     7261 aatacctcat cgagcacgat gaagacatcg aactcattga gaagtacatg ccggttgcgg
     7321 tgttcgaccc ggcgccggta gcgcaactgt atgaggaaat cggagacagg ggcatcatcc
     7381 ggggattcgt gtggggtgat caggcgggct gctggcagca tgcctgttgt ctggaggatg
     7441 tgaacaagct catactcgcc tgtttcgaca aacccgactg ggtacatcgt ctcctccgat
     7501 cgctcctcga gaagaagctt cagttcatcg cagaactgaa aggcgccaga tttgacctca
     7561 ttgaaaccgg gggcggcgcc gcatcttcca ccgtcatttc cccagacctg caccgggagt
     7621 tctgcctgcc gtacgaccgg gcacttcacg acgcactgca cgaccttgga tttctggtca
     7681 cctaccacac gtgcggaggc acgcgtggta ttgaggaact tatcgtggaa aacggctgcg
     7741 atgtttccga gacacttgcg ccacgaagta tcgggggcaa tcaggagccc tgggaattcg
     7801 cggagaagat tcggaaccgc gtggccttga tcggtggaat ggaccagtac aattgcctca
     7861 ccaccggcac accgcaatca attcgccagc acgtgcacac gctgttcgaa agggtcggca
     7921 ggaacggcgg atatgtcctt tcaactgcgg atcatttttt cgatacccct gtggaaaatc
     7981 tgaacgcgta tgcggccgcc gcgagggaat gcacgtacag ttgaggcgtt ttccctggtg
     8041 gatctggccg gaaaggtaac caacatgacg catcgagagc gcttcaaccg gattttccgt
     8101 tatcagccgg tagaccgtgc cccgtactgg ggcacaggtg gatggtcaga gacaatgcgc
     8161 cgatgggttt ccgaaggtat gcctgaaggc gtcagtctat ccaaatactt cggtgcggag
     8221 gagacaaagg gtattccggt gtattacggc ccatggccgc atttcccgta tgaggttctc
     8281 aaggaggaag gtgcaacgca ggttgtgagg aaccacgaag gaatcatcat gcgggtctac
     8341 aaaaccgatg gtgaccggag tatgccccat ttcctggaat tccccgtgaa gacgcgggac
     8401 gactaccgga caatcatcaa gccgcggctc tgtcagaacc cgagggacag atttccggct
     8461 gattggaacc ggcggtgcgc tgagtggaag gaacgaaccg accccctttg cctctttggc
     8521 gaccgttggt ccggtttttt tggcccgctg cggaatctca tggggctgga aaacctgtcg
     8581 tacgcctttt acgacgatcc ggcactgatc gaggaaatga tggatgacat ctgcgacaac
     8641 atcctgttga tcacagagca gatcttgaat gataccgata ttgattactg ggcgttctgg
     8701 gaagacatgg gcatgaagac cgggcctctc ctcagccctg agttgttcga tcggttcatg
     8761 gtcccccggt accgcagggt caccgattac ctccgatcgc gcggcgttga ccttattttt
     8821 gtcgattccg acggcgacgt gcatgctctg attccgttgt ggctgaaggc gggtgtcaac
     8881 ggcctctggc cgtgtgagat cgccgcgaac atggaacctg cgccgataaa gaaccagtac
     8941 gggcgcgatc tgctcctgtg cgggggaata gataaacggg cgcttgcggc cgggcccaaa
     9001 gcgatagatg aggaattgaa aaaggttcca cctcttgttg tcggaggtgg gtacgtcccc
     9061 atggtggacc attcctgccc gcccgacatc tcttggtcca attactgcta ttacatggaa
     9121 aagctgaagg aagcgatccg aatcgcgtaa aatcgcgcgc acggccgttt cctccgaggt
     9181 catcaatccc ttgtgatgca caccggagga tgcctccgcg ttccgaatcc gcgatcgcat
     9241 ctcttgacta acgggaagcg agtgtttact ttgatcttag gctgtacggt gtgatcggca
     9301 ttacaaaagc cgcgaggctg ggaaagatcg atccggaaaa acgccgccgc agcaagcttc
     9361 cgaacgtgtc gcgtgagcgt ccccggggca tagtccctgc cgcgaggaca aactaaggag
     9421 ttcccggtat gacgacaacg aagcgtgatc ttgcgaaata cgtggcgaag gacaccggtt
     9481 gcaaacaaag cctcgcggcc aagatggtgg acagtgtgtt cgaaggaatg cgcgagagcc
     9541 tgatgcgcgg tgaccggatt gaaatccgcg ggtttggcgt tttcgaggtg cgcaacacgc
     9601 gcccgaaacc agcagcgcgg aatccacgga cgggcgacat cattcaggta cctgcgcggc
     9661 gcaaaacaca cttcaaaccc ggcaagattc tgaaggaaga actgcgtcgc ccgaggtaga
     9721 ctttcctcgt aattgacgca ctgaccaggc tttgagctgc tgggagaggt ggtttcccga
     9781 atctggaagc catctctttc tgtttgtttg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nctcacgctc gggggaacct ctgcaaacat ggcggtaacg gttgcctcgc
     9961 tgggggtgcc ccgcgtgctt gtctatgcca acccgctgac aaaacccctt gcgcaagcgt
    10021 tcccttccct ccccaatctc ctgacaatcg gccctgacgg caccgttggc tcgccgcgcg
    10081 acgtggctca gggcgacgac gtgtttgcca tacactggat tttcgagtat cagaaggatg
    10141 acacgatacg cattgacggg cagacactca ccgccccgag ggcgaacagg ttcattccct
    10201 cgtggaaccc ggcaaacaac cagcttcgac tggcccccgg cttcaaagcc acgtttctga
    10261 aggacgcgga ccgattttcc cacctgctgg tctcagggtt tcacatcctg agcgaccgct
    10321 atccggatgg caccacggcg caggagtgca ttgtcccggt ggcggattac ttgcgcctgg
    10381 tgcgaacgcg agctccccat atgcgcgtac actgcgagct tgcttctatc gccggcaaaa
    10441 tcgttcgaaa gggggtcctc gaccacatcc ttcctcagat ggacagcatc gggctgaatg
    10501 aagtggaact ggcaacgtgg ctagaggaaa tcggcgccgg ggagctcgcg gggaacgtac
    10561 gcgatcagaa cgcgccggag gcaatccttg ccggacttaa cagactcgcc gatgccaccg
    10621 gtgttccgcg catccatctg cacaacttcg ggtactacat ggttcttgcg gaaaaaactg
    10681 cagggtcgcc ggaaggaatc cgtcagggcc ttgcttgtgg agcgtgcgcc gccgtggtga
    10741 gggccaagac ggggcgccct cccaagtcag acgaggtgac cagtttcgtg gaccttcctt
    10801 tgcgggcgga ggcattcgac gcgatgcgga ccctcgcgcc gtctgcgaag acgccggatt
    10861 tcgcgttgac gggggtggga accagcggag gacggaatct ggtgtttgta ccgacaagaa
    10921 tcgtagaacg cccggcgcgt accgtggggc tcggcgacac gatttcggca agcgcatggc
    10981 ttgcggaggg ggactaagga gcagaaaaaa cggtgcgtcc cttctcggga gcgctgatct
    11041 gagcgacaag agcggcgagg acggcaagac ccgcacacat gacgtacatc acctgatatc
    11101 cccatatctg ggctacctgg ccgctgacca cgcttcccac cgtggaacca agcccgtacg
    11161 cgagggaggc cagcgcaaac tgcccggtcg cggaacggcc gggtggcgcc agttccgtta
    11221 caagagtgac tgctccgacg tggtatgcgc cgaacgtgaa tgcatggaac acctgtcccg
    11281 cgattgcccc ggtcggggtc ggccagaccg cgtaaagtgc aaaccgggcc gctgaaccca
    11341 ccagcccaag cgtgaacagc catcgcgcgc cgaagcgccg aataatcctg ccggtaaaga
    11401 acatcatgcc gatttcggca acaacgccga tagaccacgc aacgcctttc agcgtatcgc
    11461 tgacgccgat cttgtcgagg tagaggctgt agaggttgta atggccgatc gccgatattc
    11521 ttcccaggaa cgcggctaca agaaacacaa tgaaaggtcc ggtcagagaa cgccagaagt
    11581 gccggtcctt tacggcggcg ccagggcggt tgatttcttc gctggaaaac gttgcaagaa
    11641 caacaagcgc ggcgatttcc gcggcgatga acacaaagaa ctggcctccg gcaactccgt
    11701 gccccagcag ccagcccgct gcagtacttc cgacgatgaa cgctgcggaa ccccacaagc
    11761 gggttcggcc atattctccc ccgtggatcc ggatgtgggt gagcaggaga tcatcgagca
    11821 acgggatgac gggcgtcatg aatacgccga gaacgattgt gagtccgatg atctccggga
    11881 ggccccgtgt aagcatgtac gcggggtaca gtgccgcgct ggcgatcata cccaggacga
    11941 gggtacgtct ccgtttgtcc gttctgtcaa cgaagggtcc ccagacgaac aatcctacca
    12001 ggcttaccag atttgatact gcgagcaccc acccgatttc tacgtgtgaa agcccggcag
    12061 cgcgtctcag atagagcggg agaaacggaa cgaatgcgcc aaaggagatg aaatgggtga
    12121 aatagagcag gacgaatcgc atgaagtcag atagtggccg gcgcagaggt gtgagtcacg
    12181 aagacgaacg cagatccatt gtggaagaat gacgataacg cgtcttcacc cgcggcgcaa
    12241 agggactcag acgacgcggc attcaatggg cccgccgtcg agcccgagaa agcagagcca
    12301 tgcttcggct actgtgaggc ctgtggcgac gttgagggcg ttgacgtatg catccagttg
    12361 tggtttgtac ttcaacttga ggttgtctgc gtcctgcggg gaacccctgt ccgtcttgta
    12421 atccaccagg acgagttcgc taccctgtgt aaatacgagg tcgcagaccc cgcgtatgat
    12481 attccttacg ccgttcgtgt cttcgcagag tgttaccggt acttctttcc agcgttgcgg
    12541 tgcgctgtat gcacgattga tcacaggaag cgagaaacaa ccgcgcacaa gcgccactgc
    12601 cctggcccgt tcctcgtcat tcagatcttc ttcctccgct tcgatcttca ccacacgctc
    12661 gatgtctgaa aggtctcccg ggtgcagttg ctgcagggcc cggtgaacca ccgttcccaa
    12721 ccggcggccc gaatcgtctt cgggagagcc gtgtaccgcg ggagtcggcg attcatcggc
    12781 ccaccgttcc cgctcatcgg ggtgttcaga agatggatag atgattctta ctctgggtcg
    12841 cgacgcctgt tctgtcgcgt ctctgcgttt ctcttcaaat ctccgcagtt ccgtctcaaa
    12901 ggccgagata acatcagcgc gttcaacggc gggcgcggcc gattgcgaat cccgaaaatc
    12961 gcgcggctgc aagaccggcc actcgtgtga ttccgacgca gactcgatga ggctctgcca
    13021 ggttgcgttc tttgtgacca gcaccctcgg aagcagcagg tagtcgcgtg cccttgtcaa
    13081 cgcaacgtag agcagccggg tcatctccgc cgattctcgt tctcgatccg cctcccggac
    13141 ctccttccag tcacgcgtgc agaaagaggc tccgcccgga agctttttca cgagcaggcc
    13201 acaccgcggg ggtgttctgc tatccccgcg caccattacc agcttttcac tgctctgcga
    13261 gctgttcaaa ggcgccccca ggtcgaggag gaacaccacc gggaactcca gccctttcgc
    13321 cttgtggatg gtaagaagtt gcacgcgccc gccggcttca tcgtccagtt cggcttcgct
    13381 ttcttccgcg ttcaacaacc gcagcgttct caaccggttg cagaaccccc ggaacgagta
    13441 ggaattctcc gcttcatagc gcctggcttc gtcaacgatc ttcaggagat tggcgatcga
    13501 ctgttctccg tcgggctgct gtgcatacgc agcgagaaca cccgttttct cgaacagaaa
    13561 ctcaattact tcggaaggtc gcagctcgtt caggctggcg cgggcctcac gcaggagagc
    13621 gaaagcccgt ccaatcgttt ccgggagcgc atcctgccgg tcgtcggtgt agtcgaaatt
    13681 tgaagcgtcc gcaatcgccg caagggcgag ctctgcgtcc gaaacgccga aaaaggctga
    13741 tctcagcgcc gcgatgacac tgaggctgtc gtgaggttgt tccagggcat gaagcagtga
    13801 gacgagacgc accacttcct cgcgttcgaa gaggcccttt ccacccagaa tgcggtaggg
    13861 aatgcgccgg tagcgaaatt cgtcttccag ctcctcagta accgcccagc gccggcaaag
    13921 gagtgcaaca tccccccaac gcgcggggcg caactgccct gtccggggat cgcgcacctc
    13981 ccacccgtcc gccaccacct gttggatcgc atttgcgacg agcgccgcct ccacagggcg
    14041 ggcgtctttc aatgcgtcgc accgctcggt ggccggttcg acccacagga cacgtggttc
    14101 cgcgtcctcc tgcattcccg cgggcggctc cagcggttgg tacgccggaa tgaacattcg
    14161 cgcctcgtcg gggaggtctg gcgatgagaa aacgccaggc cgtccaaaca cggcgtttac
    14221 ggcgcgtatg atgctcccgt gcgaacggaa attcgtgagg aggtggcacc tgttctcaac
    14281 tccaatgagg gaggcgacac gggagtaggt ctcaatatcg gctccgcgga agcggtaaat
    14341 cgactgtttc ggatcaccca ccacgcagag tctccccggc gacaacccgg attcttccca
    14401 cggaggggcg ggtccatcgt cgaccggact gatccgctga gagaggagga tgatgagctc
    14461 tgcctgaacc gggtcggtat cctgaaactc gtcgacgatg attgttcgaa actgctcttg
    14521 cagttggcgg cgcacttcag aacggttccg aacaagatca cgggccttta tcagcaaatc
    14581 gtcgaaatcc aggcacgcgg cgtcgtgttt ggcccttgcg taggccgcaa tgattccttc
    14641 ttcgggatga gtcaaccacc gaacgatgcc cgaaatcagg ttgtgcgtgt agcgcgtccg
    14701 cagctcgtcg acatacgtga gcaatggcgc cagttccgct gcagaactct ccggaagcgg
    14761 ctgcaacaga cgattgacgc gttcttcttg cgtggtggcg gcggagagtg cctctacgaa
    14821 tgcccgcacg gtttcaatcg tggcaagcct tttcccgggg agcattctcc cgcggctgtc
    14881 ccggttcgaa gcggggagca attccctgag gtgctcgatc gcgccctcat ccggcgctgc
    14941 cgcagtttcc gggccgagca tttccgatac cggatgcatt cggaaacggc gtatcgcttc
    15001 ggcgacggaa tacaggtcgt ccggcgtcag cccgagctcg acggaacgtc ggaccgcctc
    15061 gggaagatcg ggccgatgga actgggttgt gatccagctc cgccacacat cccgccagat
    15121 ttctgcggcc gcgcgctctt ccgcgacacg gaattctacg tctactcccg cttcgatggg
    15181 gtactggcgc agaattcgcg cgcaaaagcc gtggatggtg ccaatcggcg cttcttcaag
    15241 atcattcagc gcctgctgaa ccaatccccg ctgagcggga gagacgctgc tgactgtcgc
    15301 aaacaacctc gcgcgcagac ggtccttcaa ttcctgcgcc gcgcgctccg taaacgtgat
    15361 tgccgcgatt tcggtcaacc gcggcggccg ttcgccctct gacgtcggcg tcgtcagcag
    15421 gaaaagaagc ctgcccagca tggtggtggt tttgcccgtg ccggcgctgg cctcaacaag
    15481 gagtgtttcg ttcgtactcc tttgtgcgcg gtcgcgtgca tcctggtcga acagcgcgct
    15541 catggggtgc cgcctttgga ccgcagagaa aggaaacacg cgctgcgcag gtccttccgg
    15601 tacctcttcc agcggctcgt aaggcccgag agcgatccgc aggcgaccgc aacgtcacaa
    15661 tgcgcgcagg tcccgtgctc accgttgggt ggatcctgtg gaagcggagg gaagaaacca
    15721 ctccgcatgc aggggatgat cacaccgaga acgcgatcca gttcctcctg accgttgtgt
    15781 aacgtctcac cggaaaatga gagcgtgtcg aaaaccggta ccgagtccac gtcgtcagtc
    15841 gtgacggggt tcttgagata caggtaccac gcctcgctga cctctctcgc cagtgctttt
    15901 tcttgcaacc attggtccag catccgcgcg tacagcagcg cctggaggag ttgccccgag
    15961 ttcgtcaaac cattcagagt gcggggtttg cggcccgatt tgtaatccag aacgcgggca
    16021 caaggcgctt cgcgggagaa atccacccgg tcgattatcc cccggacagc caacgttccg
    16081 ccgtccgcaa gcgggagaaa tgccggatct tctggtgagg ttgcatccac tcggccgcca
    16141 gacacaccga atcccagttc gaccagtgaa ggttcccatg aatgcacgtc tcccagtgac
    16201 catgcaaccg ctgcccacgc gctttcctgg agggcggtcc aacgaatgcg ccagatgacg
    16261 ccgggcggga ccgtgccctc ccgttccagc tcacgtcgtt tttcttcggc aagttcatca
    16321 aaaagcggtc gaagatcccg cgcggattca ggacgccgtg gaaagagctg ttcgttgatc
    16381 aagcggcgga aaaactctgc aaggagctcg tggagaacaa gtccaaaatc catccccgag
    16441 aaatcaagga cgatgtcggg ttcgggcaac ggctcagttt tcaggattcg cgtcacgagg
    16501 aagcggaaag gacattcggc gtatgcttcc agtgctctcg tggaaacggg gttgtgcccg
    16561 gttcccagtg acgaaacgtg gttcaccagc gtgtcatcca cgctgcgttc cgcgaaccac
    16621 ccgtcgtacg gaccggggaa tgaatcacgg gcgcgcagga gcgccatgcc gttgcgggcg
    16681 tgccgactca ggcgcccggc aatttgctct cctgcagaag tgcccgcgac gcccgcggcg
    16741 tgtgcaagta tcagttcctg cgcgttgagc cagaacgctt tcggcctcgg aacgggttct
    16801 cgtacggccc attctccccg ttcggaaagc tcgcggtgaa gggccttttc atcccacagc
    16861 ggcgtctccg acggcgactc ctcaagcggg atgactctgc ggatgacttc catcagtgca
    16921 gaggacggaa gcttctcggc cgctgcagca tcggtccggc gggaatagct gagacgaacg
    16981 cggtcagatg ctgcagaaag agccatggca aacatggcat agtcaatcgc cttctgaaga
    17041 tcgagcgaaa ggctggcgga ctcgtggcga agaccgtcgt caggttggac tgaaagtgcc
    17101 gtgcgtgtgg tggttcgcgg aaattcaccg tccgatagtc ctaccatgac aagcgcccgg
    17161 catggacgaa tgcacgcacc ggtcaggttg gtaagcacta caccgtgcgg atcactgtgg
    17221 gtggtggagg cccttgtgtg tcccaacgct tcctcaagga tggtccgccg tacaggtgcg
    17281 ggtggcaact tgcctgcagc atcgtacgtg gcaatttcgt ccagaacacg ttcaatttgc
    17341 cgcacttctt cgctgctgcc gacctcagcg gtttgttgcc acccctgctt cactgcggca
    17401 acccatccct cccacgagga ggcattttca atggcatcac ggctggcgtc gagctcgtgg
    17461 atcaactggc gaagctcgca cgttgtgtca tgctctctgc gcagcgaggc gacgtcctcg
    17521 actgcgtcct cctccccctc cgcgggttga tgttcgattc tccacgccag agcacgttcg
    17581 tagcgcgcaa gcctgtcatt ccactcacta cgtacgtctc tgtcgggtct gatgccgcca
    17641 aaaactccgg cgatacgggc gatgcggtcc cagtctgagg aattcacaga ccgtgcctgt
    17701 tcacctggcg ggttagtagc gcgcggcttg cagacactga gcaggtccat caccctggtg
    17761 cgaaggagac cgctttccac cgcatccagg aacagcagaa ggccccgccc ggcgtctgtg
    17821 tcacaaagcg gttctccaaa caccatattc accggaatgc ccgctttccg tatcacgtcc
    17881 gcggcgaggc gttcactgtg gcgtttgtca ggaacgatga cctgaaaatc ctgaaacctc
    17941 aacccgtctt cctcgaggac tttccagatc caccgggctg cctcgcggat ctcctgagct
    18001 ggttccgaag cactggcaac gcagattgcc ggattctcgt gcacttcttc cgcgtccgaa
    18061 caaaacagat cgccgacgat ttccctggcg acgcccgatt tcgccggaat cgagcatcgt
    18121 gcgatgcccc acgccgcctc gaaaagttcg atggtcggtt ccgccaccgc gaacgcaacg
    18181 cttcctggta ccgacggaac gtacgcgcag gcgcgggaag cggcggccgc cagcgcgcca
    18241 aggcagcgct tttccaactc gttgaggtga agaaatccga aaaggaggta gaaatcctct
    18301 tccggggcgg cgggatccgt tttcagtcgc ttggttgcga gggccagaat gtcgctctgg
    18361 tcaacccatt tgttcgattg gaggatagcc tcgctcgcat ggtaaattgc cgcgatttcc
    18421 ttgcgatacc gcgccccggt gcgttcctgg agatcagagg gcgacacgca tgccttcttc
    18481 agttcgacga tctcgcgcag caccgcgcgc aggaaacttg gccgatctcg cgggtcgaac
    18541 gccgccccct caactgcaaa cgcgttcgtc tggtgtgtgg tgcgccggac cgcctcggaa
    18601 atcactgcaa gcgaggccga acgcggaagc ggtctcagcc cgcggtcctc aatccagtgt
    18661 cgggcaatct cgcccgccca gggggccaga tgggcaatcc gcacattgaa caggcccgcg
    18721 cgggccggtt ccgccgacgg gacttcctgt gccaggaaaa cgggaagggc atcgcgcacc
    18781 acatcgcgcg tctgtcgatc cggagtcagg acggtgatcc tcgcaaactt ttcaggcttg
    18841 cggaactcaa gaatcgttcg agcaagccgc gaaaagagtg cgtgaggagg tccagtaacg
    18901 atctcgtgca tgcgggttcc cgaatcgaaa tgcgtgcccg ttttctcatc gcccaaggtg
    18961 gttttctacc aagtctacgc gtttcccggc gaacggtgca agcgcgcacg acaggctgtc
    19021 ggagcttgtc ccctgccgaa tgcacatcgt acgttatatt cgatcccttt tcccgtcccc
    19081 cggggcaatt accccgatga gtttccggga tccgacgcgc gggaggttca atcatgctgg
    19141 aagacgcgcc gttagaacat gaactccaca accttggcaa cgattgctac cgcagcggcc
    19201 agttcgagaa ggcagtggag ttctactgcc gggcgatcga agaaaacggt cgcatggtgg
    19261 aaacgttttt caacagagcc ctcgcatatg ctcgcctcgg ggagtacgac gctgctctca
    19321 acgatgcaac ctacgtaatc gaacaaaacg cgagtctgca cgacgcctat tacctgcggg
    19381 ggcgcatcca cgaactgcgc cttgacgatc gcgctgctct ggccgattac gacagggcga
    19441 tttctctcaa ccccgatttc cacgccgcag tagagcagcg cacgaccgtt gtttcccgcg
    19501 aatggaccta tcgcgatttg cgtaaccttc gcgcccagat agcaatcgag ccgggaaacg
    19561 cctacctctg ttttcaattc ggtcagcggc ttctgttgat cggccatgtg gacgacgcga
    19621 ttcgctacct ggaaaccgct cgagaactcg gttttgcctt cggtgatctc tggggcgaac
    19681 ttggcaacgc gtatcgccag tcacggcagc ctgtgaaggc catcgcggcg ttccgtcagg
    19741 caatccgggt taacccgacc gacgtcgctt catgtgcgca cttgggccaa cttctgactg
    19801 aatcgggtca ttatcgcgac gcggtacaag tcttccggcg agctctcgga atgccgggcg
    19861 acgatgtggc cgcgcttttc tgcggtcttg gcgccgcgct ttacggactg cgaaaatggg
    19921 aggaggcgcg cgaagcattc cagcgtgcgc tggatacgga accccggctt cctgacgctg
    19981 cggccggaat gggtctgatc gcctggcgtc tgggtgaccg cgcccgagcg gcacaatggt
    20041 gcagagcggc gttggagctt tctccggggg ccactgacgc cattgtgctg tccgtgaatc
    20101 tcgcgctgtc ggaaggcgca cttgccaccg cgatggagat gctgtacgag ctgtttcagg
    20161 cgaattcgga agatcaccgt ctcgcgtacg tcctgtggac cgtcgatacg tacgctcgca
    20221 agcaacccgg tttcctgcct gacgaaggca gcgcagtgtg ggctcgccgg agaaaaacgc
    20281 gtgaaacagc acggcgggtg gtccggtccg cgttgaaaca ccgcaacgat ctctcgttcg
    20341 tccagcgcga tctttgtgac gaattgctcg cgtcgtgccc cgtgtgcgaa gaggccgcgg
    20401 aggcgctgta tcagaaactg atcactgcag gaaatgacgt gcttgcggtg ctctaccggg
    20461 aaattcgcca gcccgcgctg gttccctccg ccgaacggtt gcggggcgcg gcgtgcgttg
    20521 cgcgaatgca ggacgaaagg tggttggtgg acgtgtgcct tgcgtacgcg gacgtgctgg
    20581 tcggaacgcc ggaagcacct cgtgtggaag ggcttgcacg cgttctaacg cggctgagtg
    20641 tccgtggcgt ggaccacgca gaaggggttc gtgacctgta ccggttttgc ctcgagggaa
    20701 ccagagcggg gacagtgcac acgatgatcg gcctgtcgcc acgggtgttc cgcctcgctc
    20761 tgaccatcaa caaaccgccg tcaatcctcc cggagacagg ggagttcctg atccgtctct
    20821 cggaatggct tgaaaggttt gaacgagcgg ccagatgggt gcttccggcg cgggaatggc
    20881 cggaagtggg ggacgaactg gccgaactgc ggaacaacct tatcgagagt gttccggccc
    20941 cggaaggctg ggctctcgcc tcagtgctca ccaacttcgg gtctatcgcc cgccactacg
    21001 tcccgatcaa ttgaaccagc cgcaagccga tcaccaccga agtactccgc ttcttcccag
    21061 gtcttatccg ccgagttcga caaacggccg cggggttccg gttgcgtcaa atccaaacac
    21121 cacgttgccg cccgccgcct tcgcgcgctc aagcgcgcgc cgcgcctgcc agtacatctg
    21181 ttgagggtcg tcgaaatacg aaggataggt gactacgccg acggaaagtg tcgggcgtgt
    21241 ggcgattccc tccggcaacg ggattttgag ggtttccttg cggagaagct ccgctcgcat
    21301 cagagcgagc gtgtagttca tcccgacgag gagtgccccg aattcatctc ccccgattcg
    21361 ggcgatgaca tccgtgggac gataccgctt gcgtaccact ccccccatct cacggagaaa
    21421 catctcgcct tcctggtggc gatcgccgta cgcttcctta aggccgtcca gatcggcaag
    21481 cagcaggctc acaggtgggg ccgtggctgt tctctgtccg ccgatgtcca gtcgcagacg
    21541 cgccaccgtg tcacgcaacg catcctcgaa aaacgcccga ttgaaaagac cggtcaaacc
    21601 gtccgtccgg accagcgcgt ctgcctcgcg aaccgcgacc acccgcacca tgatcgtggt
    21661 gagggaggct tcagtaatcg gcgtctgaag aaaataccat gctcccgctt gcagaagctc
    21721 gaccagacgc ccttcggtct tctttcctat caccacgact atttctgtcg ccgggcagta
    21781 tttcttgatc tctgtgatcg tcaacacgcg gtcatcaaca agaaccgggt cggttgcatc
    21841 gaggaagaca gcctggtacc gcgggtcgat acaatcggcg atcttgttcg cgagagattc
    21901 gccgctttct gccggcgcat ggtctacgta caccgcgcgt cggagctgcg ctttcgttga
    21961 aggcgattca aacgcggctc tcaaatcgtt cgcgagcttc gctgccccta ccacgagaac
    22021 cgggtactcg aactgcgtca gggtgtgagt tgcttccggc ataagtaccc ccttcacctt
    22081 ccttccgaga ggacaacctt agtgtgacga ggcaattcca aagacttctt tcaagtagct
    22141 gagacttcga cgtgctgtca cttccggatc aatcgagaaa tcgaacactt cgacggaaac
    22201 ccagccgtcg tacccgatat cgcgcagggc cgcgacgatc ggagcgtagt ccacatcgcc
    22261 gaacccgggc ccgcgcaggt tcgcatcgtt cgcgtggaag tgggcaaccc agggtccgct
    22321 cgccctgatg atttccggga tgggcttgtt ctcgcccgac atggccttca catccagtat
    22381 gatcttcagg ttcgggctgt tgatgccctg gacgagtcga attgcctctt cggcggtctg
    22441 tatgaagttg gtctcagcgg gcgcgagagg ctcgaagcaa agcgttacct cacgtgcttc
    22501 ggcgacaggc acaatggaac ggaggaaatc cagcgtccat tgccagcccc gttcgtgcga
    22561 gacaccttcc atcaggttcc gttgctttgg cgaacccagg atcattctgt tgcccccgag
    22621 atccgcgcag caatggatga ggtccacgaa ataggctgcc gtctctttgc gtatcgcttc
    22681 gtccgggtgc gtaatgtaga ggcccttggg gctcacaagc agccagtgga gcccaacgat
    22741 ggagatgcca gcgcgttcgg cctgttggcg gatctcacgc cgtcgctcgg ggctgagatc
    22801 acgaactgaa tcggcgagtg tgaacggtgc cagctctacg ccgtcgtacc cgatctctgc
    22861 ggcaagccgg aatacgtcgt tcacttccca gttctgaaac atttcattgc agatggcaaa
    22921 acgcacagaa tactcctgtt attcagaggt tcgggcgaaa ggaacgtcga acacattgac
    22981 attcaggtca gcgagatcaa gaagagctgc cgtcgcccga ccgttcaacc accccccagc
    23041 ttcgccgggg ttcaaaatga gcgttgatcc gttcccatca gcccacgcga ctttgcggac
    23101 gtccgtttcg tgggtgtgac cgtagacaac cacatcaaac ctgcccgata cggcgagagc
    23161 gtcaacggca acgggctcgt gcatgaggat aatccggcgg ccccgagtat caaatgcgta
    23221 cgggccgacg tgtaacgtcc cgtatttcgc gaacgccttc gtcaaaccgg gtcgttcgcc
    23281 gtcgttgttc ccgaaaacgc ctgtaaatgg cactgaaatg cccgaaagac agcgcgcgtt
    23341 gaaaggcgct acgaagtcac cggcatggag gacatgaacg acgccctcat ctgcaaaacg
    23401 ccgcaggatc gcctcaagag cccatctgtt gtcgtgagag tccgcgagta cccccaccag
    23461 cccaattccg tcagcggcaa ctccgcgttc cattccaccc tcgtctgtgc cggatcgcca
    23521 caaccctgca taaattacgc gtggacaccg atttcgtcaa ccggaggtcc agacgcgcta
    23581 atacggcacc cgaacctgcg cgttaggcat tccggaactt cgaagggtac attttgtcat
    23641 attgctccgt ttctggcggc tttgtgtggc gtgattgcgg cgaggtgtgg caaccgtgga
    23701 acattggcat cgcaggtgcg cagttcgccc tgcccttttg gtggccattg tcgtggcgac
    23761 ggtgcctgca tggttggccg ccggtgacga ttaccccccg atccagatca cagatctcca
    23821 aacaccggtc gaacgggcca catttgttgt ccagactctg ttcgactcca cgaacggaat
    23881 cgaagacgtc taccacgccg catggacggt tgccgggaaa ctgtactacc gctccggtgt
    23941 gatcacaagc ctggcgcggc ccaggcaact tgttacgggg ctgcaggcct gtgctgagcc
    24001 gttgctgttc atgcttcctt ccgggaaacc gggtcttgtc tgggcagagg agcggacccc
    24061 caacctcagc atcgttcccg gcgagtggaa cctccggtac acggcagatg tgaacgttgc
    24121 accgcggtgg ttcaccgaag acgcgggaat cggaggagtt cacccggatc gcaatccttc
    24181 ggtggcagtt cagggagaca cggtgctggt cgcctgggaa cgggacgagc caccgcaggt
    24241 tgcctacgca ttcctttcgc cccgggacat ctttcccctc gtgcgactcc tcacgtcata
    24301 cccgtacggg gtttcgcgcc cgactgtcgc tgccgacacg gaaggtgcgt actggattgc
    24361 atggacggct gagacaccaa caggggggga cgtgtgggtt gcgcgagtga gcaggaacgg
    24421 cgcgacatcc gcgtcacctg tgcgcgcggg accagaggat gcacggaact gcgtcatcgc
    24481 cgtcaatgcg gaccgagaaa catacgttgc gtggcaggaa ggcctctctg gcggcagcat
    24541 ctacctcgtg cgcgcactgg ccaacgggcg ctttacaccg gccgtcaagg tgtccccaga
    24601 ggggaccgtc tgcgcaacac cgcagctctg tttcgaaagt ggcacgattc cctgcgtaag
    24661 ctggcgaaat gtactcactg gggacctgga aatggtccgg gtgcacggaa ccaggccaat
    24721 gggtcccgtg aatctcgtct cgggtgtcta cacaccacgg caggagttcg tcacaccggc
    24781 acaactccgt tcccgttccg gttgtgtggt gagcgccttc cttggccctg atcccgtccg
    24841 cggcgtgacg aatctgtgga tatcgagagg gaaggaatac acccctggcg ccgattcaga
    24901 ttcgaatggt ggcaacaaac cggcggcgcc ttccccgttt tttacgcggg tgtccccgaa
    24961 tcccttcaat ggctccgttc tgctggaagc ccacattcct gcgtccgttg cgtggcgact
    25021 ggagatccgt tccattgacg ggaggctcgt gcgaaagttc gaaggatcct ccgtcgcacc
    25081 tggccctgtt cgggtagtgt gggatggcgg ggactccttc ggacagacgc tggggagcgg
    25141 cacatacctc gtccgctggt ctgtggggct gccgaacggc gagatgtgga ctcataccga
    25201 acgggttacg ctggtgcggt aatccatacg gggttggagt acgcgtgtct ccgcatgtca
    25261 gtgtgcgctt ccacgcgcac ataaccctcg ctcaacgctg gcacatccac atcaaccccg
    25321 aatcgcaagg ccgggttctc cggctttttc cacgtcatgg actcaacgcc tcccgtctgt
    25381 ccgacggcga ttccgatacg gtccacataa ccccattccc gggtgctttc ggcttccacg
    25441 tggaccgtaa cattgccgcc ggccagttcc tcacccattc cagcgcgggt ggtgccgcgg
    25501 gaaacgtcaa ggcgaatcgc cggaccgttc gttatgtaac accgccccgc ggcaatcgaa
    25561 tcgagcagat ggtggttcga tagtgaatcg gcacgaacga ccgtccgaac gtcgccaaac
    25621 acgtatgacg agtcctctgc gatcgaccac caggggatct ttacctgacg tgtgcggcca
    25681 aatgccccgt gggaatcgtt ccccgccaca acacagaggt tctttcccgc aagcaggagc
    25741 cttacccact gccccatccc gcgccggaat cccgcgtcgg agaagcgtcc cccggcctgt
    25801 atccctgtca gcccttcacg cgcaaggtca tcgggcttcc acacgccgcg ccggaaaaca
    25861 agccgctgaa tgcgaggcgg ggtgaagccg ggatgcgcgg ccagcgccaa ccctccctgc
    25921 cgctgcacaa ggtcaattac ttccgtgatg gaatggtcgg ggcggttccg gaacggtcgc
    25981 tccgcgctgt cccccttccc cggaacatgt tcgtggatgc cgagggccag caggtggaca
    26041 ttccttccct ccgcattgcc gcaggatact tcctcgcccg gnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn ngtcacccgc gtaccaccct gcaggaaggg ggtacggctc
    26221 ctccgcgatg tgcacacgga acggctggtg cgatgtcccg cggtagttgt ccgccaccat
    26281 ctcccgcgag cgtcccttca ccgaataccg gatgacaggc acgatttcag ccgtggttcc
    26341 ggcggcgagg ggaggaagag tgagcagctt ccaccagtac tgggtgctga ttgcagtgtg
    26401 aaggggaaac tctgcggttg tgcaaccgga agtgccggca ttgacggtta cggaaacgga
    26461 ttcgagcagg acagggtagc gattcgcgtg gtgaacgaga agcagaacgg ggatccccgc
    26521 gcctggctcc acacgccagg gaatatccac cacgatttcc ggctcagccc tgcgtatgcg
    26581 gctgagccag tatcgggggt aatgtgtttc tgcgtagaga agcatgggaa ggagtcgcgt
    26641 cagtcggttt cgacgctttg cgagacggtg cttcggatgc gcaccaatcg cctcggtaca
    26701 acggaggcgg tccccagtgc cgccacaacc agtgcaacca cgacccaggg cggcatcacc
    26761 cagaagccgc cgatgacgca gagaaggaat atcctcggaa tgcggccaag catggtcgcc
    26821 gcagtgtacc gttctatcgg gtactgcgct gccgcggcga ggaatcggac gagaaaaaag
    26881 ggcagtggca ggagggcgaa tgcgaaaacc gtcccgaatg gccatacgcg aaaccattct
    26941 actgcgcgcc cggtgaaaga atggttcaag aagccgcgga gtcggtcctt gcgtgcaaac
    27001 cactgaagca cggtctggtc aatcacgcag gcgacgcccg cgccgattgc ggcagtaagt
    27061 gctaccagca acgcagcgtc gtagagggag gtttttctga tgagcatctg gacggcggca
    27121 attgccgctg gctcattcgg aaccatcagc atcgaatgcg tcgggaccga ccagaacagg
    27181 aaaggaatca ggactgcgcg gtcagggtgg agcgaacaga ccgcggcgcc gacaagaagc
    27241 cccagcagcg ctcttctgat agacttgctg gaccacagac ggcgacccaa ttgcaaaatg
    27301 gtgactccta taatggcaat tcgactcgct gaatctggtg cccgtcgggc aacaatgcgg
    27361 ttaccgtctg atatcccgca acacgcatcg acgcgcgcac ttcgtccagc cgtgccccca
    27421 cctgatcagg tgcgtgggaa tcatctccga aggtggccgg aatccccatt tcgcgcgctt
    27481 tcctgagtag ttccgcgcgg ggatatatct gcttgcacgg tttgatgagc ccgcgcccgt
    27541 tcacgtcaag gatgacgttc cattgcagtg ctgccgctag tgttgcctcc tccgcttccg
    27601 ccacatcgtc cccggaaatt ggcgcagggg agaatatgtt gatgaggtcg agatgcccga
    27661 gtacgtccgt cacacccagg gccagcaacc ccctcacgtt cgtgtagtac gaaactgcaa
    27721 ggttctcgta gcctccgcac ttctgagccg cctcgtcata ccactcccgg cagtgatcga
    27781 atccgattcc cgccacataa tgcaccgacc ctaccacata gtcgaagggg taccgggcga
    27841 gaaaatccgt gagatactcc tcttcgtcgg gaaggtactc gacctctatg ccgagcagaa
    27901 gcggcagttc atctctgtac gtaaccttca gctcttggac cgtatcgacg taacggtcga
    27961 actttcgaac agcttcgtcc cacgaaggct gatcggggta aggataacga gggggtgccg
    28021 gcatgtgctc gctgaacccc aatcctgcaa acccttgccg aaccgctgcc tggacaaaat
    28081 cctcgagccg ccccgttcca tcgtacagcg tgtgcccccc gtgatacgac acaagtgacc
    28141 cacgttcagg acttcctgcc acaaatgctc ccacatatat tcgttctgcg tgtgcgaagt
    28201 tcgtctagta catgataatc gcctcatcgg tgtttaccaa cagaaaacga tcgaggaagt
    28261 cgagccggcg caacgttaag cgccgttccg cagccatttg acgggaattt gcctgcatga
    28321 tccatgcgca gagcaccttc gcggccatac tggcggtcac gtccgccgtc acttccgtaa
    28381 tcgcattgta cgcctggttg cagcgcaaaa cgccgggagc agcctgcttt gcggggttga
    28441 tgacatctat cacggtgtgg acgttcaccg gaatggcaga aatgctgacg gcgtccgcag
    28501 cgggcaagat tctctggtcg aaagcaagtt acctcggaat actcacgatt cccccgctct
    28561 gggttacgtt cgcgctcaca ttcagccggt tcgaaagatg gttgacccgg cgtgttcagg
    28621 cagtgatctg gggtgttcca gcggcgcttc tgcttctcgt attcaccaat gagttccacg
    28681 gtcttgtctg gccttcgatt gtgccactta ccgacgaacc gggttccgtg ctgatttacc
    28741 agcacgggct cgcgatctgg ttggtgatgg cgttttccta cacgggtgtc gtcttcggct
    28801 gcatatggct cttttacgcc gcgatcagtt cgcctcggtt gcggaaaggt cagaccgtgg
    28861 cgctcgtcat cgccgcgttg attcctgtgg caagcaactt cgtctacatg gcggatttga
    28921 gcccttatcc gggcttcgac ctcactccct tgtcattcgc ggtctcggga gcagttcttg
    28981 cgtggggtat cttcggcgtg cgtctgctgg acgttgttcc ggttgcgcac cacgcccttt
    29041 tctccggtgt tacagacggc gtgcttgtaa tagacctcga ggagaggata gtcggcgtca
    29101 atccggcagc gacgaggatg tttgggctcc agtccagccc gctcgggaaa agcattctcg
    29161 aagcgacgac acatgttacc gggctggagt tcagattcga aaacgggcag cgcgaacatg
    29221 gcgaagtaac cttccgtcgc ggcgaagatc agtgttgggc cgacgtccgg gtctcgccac
    29281 tcgtcgacgg ccgcgaacgg aaaatcggag agctgatcat tctgcgcgac gtgacggccc
    29341 gcaaacagac agaggaacgg ctccgccttg cgcaggaagc aaccgccgcg gccaacagag
    29401 aactgactgc ggctattgcc caggcgcggg agatggcgga gcgcgcggaa cgggcaaacg
    29461 ctgcgaaaag cgagtttctg gcaaatatga gccacgagat ccgaacaccc atgaacggcg
    29521 tgttcgggat gacgcagctt ctgcttgata ctgacctcac ccgtgaacaa cgcagttacg
    29581 cagagatgct gcggtcaagc gcggagagtc tcctgagtgt cattgacgaa gtcctcgatt
    29641 tctccaaaat agaggcagga aaactggcgc tggaaatcac tgaaatgaac cttcgcacgg
    29701 tgtgcgagga tgtgtcggca gttgcggcgg tacatgcaca ggccaaggga ctggaattcg
    29761 tgtgcgacgt ggcgcccgac atgtgcgaga atgtgcgcgg cgatccgacg agggtccgcc
    29821 agattctgaa caatctggtt ggcaatgcgg tgaagttcac ggcacaaggg gagatcgtgc
    29881 tttccgcgcg aatcgaatcg cagatgaaga aagccctgaa cgtccgtttt tcggtattcg
    29941 atacgggaat tgggatcccg aaagagaaaa tcgggatgct gttcaacccg tttacgcagg
    30001 ccgatatgtc gacaacccgg cgctttggag gaacagggct cgggttgtcc atttccaagc
    30061 gccttgcaga gatgatgggc gggcagatag gcgtcgcaag cgaagaaggg aaaggatcgc
    30121 gcttctggtt caccgcgcag ttcattcgtc cggaagccgt tgaggagcgt tcgccagaca
    30181 cgcacgcgca gttggccggc cgacgcattc ttctggtgga tgacaacctt tccgcgcgca
    30241 gagctgcctc atctctcatc cgggcggccg gttgcgtgtg cgaagaagcc ggaagccttg
    30301 cggaggcgcg ggatatcttc gcggcggcac gccagaatgc ggaaccctac cacgcggtgt
    30361 tgcttgatgt tcccctcgac tttggagacc cccgagcggc cattccccgt atcgatcacg
    30421 gatttgacgg aactccgatt gttctgctgg ttccgttgga caagcggggg cgggccgccc
    30481 aagtggaatt cgagggtgtc gccgcatcag ttacgaaacc gtttcgaaga gacgatctcc
    30541 tccgctgcct gacatcggtt acaggctctt ttaactcagc acacccgccc gttccacgat
    30601 ttgtgccccc gtccaccgcg aagcactacg cgcgcgtgct cgttgcggaa gacaacccgc
    30661 tgaaccagaa actgactcag atcatgttgg agaaactcgg ttgtgaggtc gtaaccgtgt
    30721 caaacggtgt cgaagtgctc tccgaactcg cttccaaatc gttcgacctg atcctgatgg
    30781 attgccagat gccggagatg gacggctacg agacttcgcg caggattcgg gagtctaccg
    30841 tcccgaggat ccgcgagatt ccgatcgtcg cgcttaccgc cagcgccctc attggcgacc
    30901 gcgaccggtg tattgaagcc ggtatgaacg acttcctgcc caagccactg cagaaaagcg
    30961 acctagtagc tacgatctca cggtgggctt cgggggatcg atccggcgca aatggcagat
    31021 cagggaccgg agaggtcctc gcgttcgatc cagagaaact tatgggccgt gtgggaggta
    31081 actttcacgc ggcgcgccag ctagtggaag cgtttttgcg agatgcgcgc gatcatgtgg
    31141 cattgctggg caacgcggtt tcagaaggga atgcgtcgct gctggcggcg gaagcgcacg
    31201 ccgtcaagga taacgcgctg cacacgtgcg ctcctgcact tgcgagtgcg gcggctgctc
    31261 ttgaagcggc gggcactcag gcggatttcg cgcggtatgc ggaactgatt gaggctgttc
    31321 agcggcatca cgcggacgca gaacgcgaaa tgtgcgtgtt catcggttcg gaaccgaaaa
    31381 ccgagcaaac ccttatttga agcgcgcggg cagtcacgca ctacccgcgc gccgctcaga
    31441 acgctcatct gccgttcgcg tgacgggctc ttttcaatcc ttcccgagat acggcgtgca
    31501 acccggtatc gtcgcgtagt tcttgaacgc ttccggaatg cttccacccg cgtcctgtac
    31561 cgcgcgttca cgcatcatgg cgcacacggc aggctctttc tcggcgaggt tggtcacaag
    31621 gttcggatcc tccctgtggc gatacagcaa cgctccctct ccccagatgt tcgcgttgta
    31681 ccaccaggta tcgtcgatca ccgtcaccag tgggccccag gccaccgtaa cgtactcgcg
    31741 caacggcttt tttgtatcac ggatcgcatc ccagacattg atcccgtccg gcttctgctt
    31801 cggctccact cccgcaagcg caagcatggt tgcggataag tccatgttgt agatgagctt
    31861 gtcgcatgtg gttccggcgc cttcaccttt cgggtggcgg atcatgcaca cgagctgggc
    31921 gaccgctttg gtcatcgggt gaccctgttt gcttacaagg cctttgtcgc ccgggtcatg
    31981 cccgacatta tgcccgtggt cgctgacaat aacgaccaac gtgtcttcca gcctgccggt
    32041 agtgcgcagt gtttccatga agaaaccaaa ccaccggtct acaagggtgc attcgccggc
    32101 atagtttgcc tgaatgcgcc tgagctcgcg ttctgtcaac cgctccttcc accggcggta
    32161 cggagactgg atgacgttcg cgcagtcgtc atccgggtcg tacaacttcc ggtaatggtc
    32221 cggggggtcc cacggctcgt gcggatcgaa cgagtccacc gtgaggaaaa acctctcggc
    32281 gtcctgatta tcccagagcc accgtgatgc ttcggagaaa agccgggccg ggtagtaatc
    32341 cttctcggtc atccggtagg tgttgttgcg gagatacgag gtcatgaacc ggtggaacca
    32401 tacgtcgttc ttctggctcg gcgtgctgcg ggccattatc tctgcctcga gcaccggagg
    32461 tccactcagg tacttgtcgg attcctgtcc acggatccac acccattcgt caaagccgcg
    32521 gtggaagttt ttcgacggtt tgaactgatg atacgtgtcc gtgatgaacg ccgtgcggta
    32581 tccctgttcg ctgagaagct ccgagagcgt gtcctgttcc tcgttgatgg ggccccaccc
    32641 gggtgccccg acaaagtccc ccttcagcgg ccggtggccg tggaacggaa acacccgttg
    32701 tccggtatgc agcgctcgcc gcacgggaag cgtcggaagg ctctcgggat acgcctcggt
    32761 gaacaacaca ctctcccgcg cgattgcgtc aaggtggggc gtgtggatcc acgggttgcc
    32821 gtaggcgccg acatggtctt tcctgaacgt gtccagaatg acaagcacga ggttcatggt
    32881 tcggtttcct ccggtgtgtg gtggcaaaac gcgatatccg caaatatggc ggcggagaga
    32941 gtccgccgca acgggacacc gggttacccg gagggtggcg acaaggggaa gtaaccggga
    33001 attgcggctc agtcccgcgc cgaggctttc cgctcaaggc ggcggcgcgc ccatttcgtc
    33061 acgaccgggt agagccacag cacgacgccg gtgagtgtca caatggccag aaatacgccg
    33121 gtgagatcgc tgtaagtgaa cgcgagttcc cgaggaagga atgcctttcc ggtgtgcaga
    33181 tccagcatcg tcacgtaagg acggccccgc acatgagcga cgacgatgcc ggtgattgcc
    33241 atcaatgtca gtccgatgat gccggcaacc ccgatccaaa ggtggagctt gcgattccac
    33301 cgcatcgcca ccttcattgc ggtcttctcg ccgaggaggt agtcgtatac cgcaagacgg
    33361 gaaagggatt cttgcaatgc ggcctgccga cgcgtaagtt cctccttgtg aagaagcagg
    33421 tccagaaggt cgcttatctg ctcaaccagt tccttcagga attccacatc ctcatcggaa
    33481 aattccttgt ttccagtctt cttgttcaat gcctggacaa ctccgatgca cgtaccgcgc
    33541 tggttgatga tgggcatcga caggaggctt ctggtccgat accccgttcg tttgtcaatc
    33601 tcgcggttga aacgggggtc ctcgtacgca tccgccagcc gcagggtctc gcgcgaggta
    33661 aacactgcac cggcgatccc ggctgtggag ggtatcctga tctctccgta tgtgaggccg
    33721 ctggcgatgt agctgtagag ctcgtcggta tctgagtcgt ggaagaacac cgtgaagcga
    33781 tccgcctcaa cgtactcgcg cacctcctcg ttgatgcgct gaatcacctt ggcgcggttc
    33841 gattcggcgt tcatgatttt gctgacacgc agaagcgttc gcagtttgcg tatctccgca
    33901 tcgcggtcag cgggagattg gctggcggtt ccgttcacgc gcaactcctg acgacacaga
    33961 aacgaaaagg cgccctgaga gggcgcctgg aaccgggaac gagtcaggtt ccctgctgtt
    34021 gtgcccgcac ggaatcggga acaaggtcgc tgtgctcgct caacacccag cgacgtatca
    34081 tatccacttc tgactgctgc aactggaact ctttcggtgc atgttcggcg attgtgctgt
    34141 cgcccatcag catgttgtaa agggcgatat cgtcaaacag ctgcgtactc gagatgagcg
    34201 gcaggaggtt tttgggttcc gtcagatcga ggttgccctt tgcgtccggc gcagtgtggc
    34261 atttcacgca tttctgatcc agcaccttct ggatctcgcg ttcctgcgcg ctcatcgccg
    34321 cgagtttcgc ctcgcgttcc gcattgttgt tgccgcacgc gatgagcgcg cagcccatac
    34381 tggcaataat ggcgacaatt gcacctgttc gcattggcga aactcctcat gatggatgaa
    34441 acggtcgaac tcccacgccg catactcgca aagaaatata aatgggcctg agcaaagtcc
    34501 caagcgtgcg cggtggcgca cacgcagccc ctgagcggtc accattcccg cgaaagaggg
    34561 tacttgccgt actcacggcc cgcatcgatg tacgcgagga aattggctac cgtttgctcg
    34621 tcgcaggtgg gccattcctg gagcccggcg ctcgggcaca acacgaatcc cccgccttct
    34681 cccgcgtcca gaatggcctg gcgaacccgt tcacgaatct cggaaggggt tgacgtgtac
    34741 atctcgtgtt tctcgagatt cccttccaac gccatcctgc gtcctacctg acgcttgatg
    34801 tctctcagct cgatatcccc ccagggggga ggttcgagcg gctggagaac atcgttgccc
    34861 gcgcgaatga agccctcaag cactgctttc gtattcccgt ggcaatggca ccatacgatg
    34921 cccccgaact catggatcag ttcgatccac tgccggtcat agtgcacgac gaaatcctcg
    34981 aagtcccgaa cagactggct tggtggaatg cacacttccg gccccacata cgcgaacagt
    35041 ggccccactc cgcgctccag tatgtagcgc aggtactcca gcgtcatttc gagttcccgc
    35101 tggatcattt cgtgcaggag cgggcgctcg tcaatactcc agaacgcgaa cccctcaggg
    35161 ccgagccggt cctgaaccgc atacatggga tgggaaacac cggacatgat gacaccgcgg
    35221 tcgcccatct cccgaactcg ctcgaaaaag ccgctgactt cgccctggag gggcccgaac
    35281 gggatggaca gccagcgttt cgcgtcctcc ggggactcga tcaggtgctt catgcaatac
    35341 cccggtttcc cggaccgatt gaacacatcc actctggtga gcggcccggc tggggtgtgg
    35401 atcgtggtta cccgttcttc gaattccgcg atgcggctgg gacgctcctc atgcgtaacg
    35461 cggcacaaca tctcccggcg ggggccaccc ccccacccac ccacaaggtc ggttttttcc
    35521 cgtgcggctt ctacgatggg agcgtacgaa ggatggatcg gttgcgtccg atcatccact
    35581 gcccacaccc gcaccggtac ccggtcaggt acctgctttc tgtacgctgc catgaggcgc
    35641 tcatgactcg tcatcggcat caggtggttc ctcgttgagg ggtaccgcag tctgcaacaa
    35701 aaaaggcggg gtcggacaac gccccgcctt gaatctcaca ccgctgctta cgcaagctcg
    35761 atgtcctcgg cgtcttccgg aaccatgtcc agcgacgcgc tggtttgcct gtaccacacg
    35821 acaagcgcaa ccgccgctcc tacaagggcc agaagcaaga ggagcttctt catagccttc
    35881 ctcccacgga aaaggtccac tgcccactgg ataaaaaata cggcaatttg ggagaaaaag
    35941 acagccgttg cgtccatttt tcctgcaccg gttgcggcaa ttcagcggaa ccgcggagcg
    36001 ctcctcgtta ttccattttc cgtgcttccg cgcacctgct cccagtgtcc cttgaggagg
    36061 ggtcgctggc ttcgtttctt cctgactgtc aacgttcgag cacggcccgg cgaaagaggt
    36121 gacgagtgat gagtgcggat attcggggcg aatggccaaa ctacggggga gctcttgacc
    36181 caaccgtctg gatgcggatt cgggcctcca tgaaccacga ggagggcgac cgggtgccca
    36241 tctgggacta cctcgacaac cggcccacgt ttgagtactt ccacagaccg ggcgatacgc
    36301 atgacgaaac catgcgacgc gcgtatcacg aactggggat cgacttctgc cggggatacg
    36361 ggagcagttt ctcgcgcgag gacgaaggcc gggcgagtga ccatgaagag tggaaaatct
    36421 cagggctcac ccagtgggcg acgaaaaaac cgatctcgaa ctatgccgaa ttgcggtcgt
    36481 tccagccgcg gctttttacg gaagtagact gttcccgctg gctgaagcag acgcaggaag
    36541 cccaggcgcg gatggcgccg ctttcctact tcgtccccgg cgtgggaaca gggttccacg
    36601 aagtgtacga cctcatgggt cagcagcttt tcagctatgc gatatacgac gagcgcaggg
    36661 aactcgatcg tctcttcgaa gcgtatggct acaattcgac gctgatagcg cgtcatgccg
    36721 cgtcgacaaa accgggcccc ctgtttttca cgtgggcgga tattgcgtac aaagggcgtc
    36781 tcctcttttc ccgttctttc ctccgcgaaa cgttcattcc gatgttgcga agcgtctgcg
    36841 aaccccttgc ggaggcaggg atcaaagtgg tgttccattc cgacggtgat gtcacggaaa
    36901 tccttgatga catgcttgac gcaggcatta acggcctgaa tcctgtggag ccgcaggccg
    36961 gaatggacat cgggcgcctg aagaaacggt acggcaaacg cctcatactg gtggggaata
    37021 tcgattgctc gcaggtattg ccgctgggca gcgttcagga cgtggtggaa gcagtcaaga
    37081 catgcctgcg ggaggctggt cacggcgggg ggctgtttat tggctcttca agtgagatcg
    37141 tgccgtccac ccccttggaa aatgtgctgg cgttttttga ggcctgccga accttcgggc
    37201 gctacccgtt ggccgtgtaa gcggagaacg cctgccagac ggttccatgt ctgcgcgtcg
    37261 gttttgctcg ggaaccggtc tgatacccaa gaatgcgtgg cggagaacga agacagaaca
    37321 cgcgagcggg agaccggttt cccggccgtg gaaatacgcc atccgtcctt gaccccgcgc
    37381 ctgaaagcga ctattttccc tattgcttag acaggagaat cttttcagct cccaccctcc
    37441 ggaatttctg ttcgtccgtc tggaaggggc gttctggcgt acgcacgctg ccggtgcatg
    37501 cgccgggtcg ctttttttcg cgggcgcgga agtcacgggt ggccgcgaaa cgtgtacggt
    37561 ttcccgctcg ctgttttctt ggaacgcggg gatcgtgcgc gcagttccat gcgcggctgc
    37621 gtgttcaata ggagaacggg gaggaaagat tctcagcctg atgcacggtt tcgtttcggt
    37681 acgcgtgtat ccgttcgtcc ccgggatctg gacttgatgc cggggaattc accgaggtgg
    37741 gtaaccaccg catcactagg aggcacctga atgcagcgac agcgctatgt gtggctggca
    37801 ggcctcgtcg tcagtctgat gatcctcgtt gcgggaaccg cgcacgcggt taccgcgggg
    37861 aagatcatcg ggaaggtcac cgatgcccag acgaatgagc cagttctcgg agcggccgtg
    37921 accgtcgagg gcttgcgaat gggcgcaacg actgacgccg acggacggta cttcatcttg
    37981 aacgttccag caggtcgtta cacgctgaag gtccagatga tcgggtatgc cccggttacc
    38041 cagctggatg ttctcgtgaa caacgacctg accacgcggg cagacttccg gttgcggtac
    38101 caggcagtgg aagtcggtga ggtaacggtt gtcgccgaac gacccctcat tgaaaaaaca
    38161 ctgacaggca cccgccggac gaccctgcgc gaacagctcg agctgatgcc ggtaattacc
    38221 tcggacgaca tctatcgtgc cgcccccggt atcgtgtttg acccgatcgg tggaccggtt
    38281 cagcaggtta ctgacggaac catccgtgtc gaagacgaga cacgctccgg aaacacggca
    38341 aaccccgggt actacatccg cggaagccgt cccggcgaag tgaactacta cctcgataac
    38401 ttcccggtaa gcggcgttct cgccctcccc ggaaaggcga ttgaggaaac caacctcctt
    38461 accggtgggt tcagcgcgca gtacggcaac ggaacctcca tcatcaacac tgtgacaccc
    38521 accccgggtg cagcgtacac cgcctcgttg aactgggaga gtgatcttct tcccggcctg
    38581 gtttcggagc gttataacta cggaaccaac gtggttaccg cgagcttcgg cggcgcggtt
    38641 ccgggaacca acaaccgcct gcgcgtctgg gtcctcggcg acgggaatat gaccgacgac
    38701 tgggatcccc gtctttccac acaggacgcg aacttcatcg aaaatggtat ccgcgagttc
    38761 acccaacgcg ccaacatggt ggacacgctt cggacgttct gggccacgct caaaccgtgg
    38821 gacgtgtttg aaggtgatcg cctcatcctg ccatcgcaca agcaggacat gtacacgttc
    38881 atgggcaagg cgatgtacaa cgtgggcgcg ttccagttta cgttgagcgg ctttgcgtgg
    38941 cggcgtcagt accagcgttt cagccccaac ggcctgttca tggactggga caagaacaat
    39001 ttccggaaca tgccgtaccc caccaccatg cagcaggcaa cgttgctgca tcgcaatctc
    39061 gttgccttcg gtgttcggtg ggcgctgagc ccgcgtacca tcgtcgacgc gaagctgagt
    39121 ttctcgacga ccgccaccac gacgggttcg ttggacctga agagcggttc attcggtctg
    39181 ttcaacgatt tcgattactc ctggcacgaa tatgcgccgt cgtggtcgcc cttctatgtg
    39241 gatccggcgg actgggctac caccaatcgc gcgaagtaca cacagcaacg gcgcgaagtg
    39301 tattacccgc ttggcgtgac gaacctgttc tttacgagcg ggaattcccg cagcgctaac
    39361 gcggttgccg gaaacaacgt tgtaggacag ttgcacgcta cttcacagat caataacaac
    39421 cacgaactga gtggtggtgt cgaaatcacg cgcagcatga ccaagcagga ccgcctgagc
    39481 gatccgtggt cgggcacccc gttctttgac cagtggggtc cgttcaatcc gatctacggc
    39541 gcggccttcc tgcaggacaa gatcgaatac gaaggtctca cggttacggc cggtgtgcgc
    39601 atggactact acgatccgga tgcgtacgtc tggtctgatc cgctgaaacc cggtgcggga
    39661 agcatcatgc cggacggcag catcaacgtc aacgggcggc ctgacgtgaa cgcggacaaa
    39721 gtgaacgcct tccgcgataa gcgcgtagac ccgaagatga cgctcagccc tcgtctgggc
    39781 atcagccatc cggtgtcgga tgtcgggctt gtgtacttca actacggtca tttcgtcacg
    39841 cagccggcaa acccgtacga gagctatgtg ccggatctca accggtcgaa ctcccgtctc
    39901 ggtaacccgg atctgccgtt catgcgaacg atttcgtacg aaatcggtta ttcgcacgaa
    39961 ctcgtcacca acaccaagct cgacgtggtg ttgtatcgcc gcgatatacg gaatacgaac
    40021 acctatcagc gcattcccgc aatgaatggc gcgtacacct acgcgatggc gtccgccacc
    40081 actctggtga atggcaagga agtcaacatc gggtacggca actccaaagg cctcgaactc
    40141 atcctgacga gataccgggg agccgaaggg atgttcagcg gctggctgag ctactcgtac
    40201 ctcaaggcgg aggcgattta ctccgacgcc aacgacgcgt acgagcggtt ctcccgaagc
    40261 gcgctcgacc ccgaaaccgg cgaactggcg tatccgcctg acgtgcccgg aatcgccgac
    40321 tacgatcgca cccacagctt caaggcgaac ttcgatatcc ggttccgcca gggcttcggg
    40381 ccgcaggtgg ccgggatgtt cccgttgcag aacatgggta tcaacatcct tgaagtcatc
    40441 aactccggat tgccctacac gcgcatcaac atgagcggca aaccggtcgg tatcaacaac
    40501 gggttccgga aaccgtggac ctacgagacg gacctcatgc tgaacaagca gctccggctt
    40561 gcgaacgttc cggttacgct ctacgcgaag gtcgagaacc tgttcgatac ccgcaacgtg
    40621 ttgaacatct acgagcgcac gggtaacccg atcgacgacg gaagaatcat gcctcaggcg
    40681 cagccgaact ccgttacacc tgccagcccg acatgggtgt atgaaggcgt gaaggacggc
    40741 atcgacggca gcacgcccga cggcagaatc agcatcgccg aagacgaaac agcgtatcgg
    40801 aaagcgtggt caatctacgc ccgcgacccc ttgttcttct cgaatccgcg catcatccga
    40861 atcggcgtgg ggatgtcctt ctgagggtgg atagatgcgg atttcaggaa aacatatacg
    40921 aatgaaggag tgccaaatga gattctcgcc tgaccggctg aatgcacgcc ggttcccgtg
    40981 gatcggcatt atggtcgcgg tgctggttgt cgctggaatc gcatccgcca ttcccgcgcg
    41041 caacgcagct cgcgtggcgc ggtccggaag gatcgcgtcc gaccatgtgt cgctcgaaat
    41101 gcgatggctc gatatcaaca atttcaaatg cccgattacc aactacgggt tcatcggcaa
    41161 taacaacgcc gagaaccggg ccggtggcga atggcccgcc ggatcgggtg agtattacct
    41221 ctacggcggt ggcatctgga tcgccggaac cgtagaccag ggtcccttca ccgaaaacat
    41281 cggtgggaac accgtctaca cgttcaggcc gtccacaaca aacccgctcg gttggcaggg
    41341 ccttggcgct ggcggccgcg caattcttac tgacccccgg gcggtcatcg ggtacgaacc
    41401 ctccggcgga accgcagaaa tggcgccgtt gacaaaggtg ttcatgagca ccgagtcgga
    41461 ctggccgttc ccgaacaccg tgtcgattct ggatacgtac acggagtacg acgatcagga
    41521 tccgcagcgt tggaacaccg gaaccggctc gaactccggg aaggacaccg gcaaatggtc
    41581 cggtacgccg actgccagca caatcgccac ctacgggctt ggcgtgaagg tgaagcagac
    41641 gacgtattca tggaactacg ccaacaactc ggatatccac ttcgtggtgt atgacgtgga
    41701 gaacatccgg accgacggca aaacaatcaa caactgcttc gtcggtgttg tctccgaccc
    41761 ggatatcggg aacgaggcgg cggacgacat ggcagggttt gatgcgtcgc gcaacctcgg
    41821 atacgcgtat gacagcgact tctccgaggc tgagttccgt ggcgttcccg gcttcatcgg
    41881 gtatcgcttc ctgaagagcc cggtggccga gcacgacatt gacaagaacg gcgacggcgt
    41941 tatcgacaac aatccggtgc tcatcaacag cgtcccggtg tacgatgtgc gcgcaggcca
    42001 gcagatcggc ttgcacgcgt acaaaatctt cggacgtatc gcgggcgacc cggatactga
    42061 atgggaatgg tacatgacaa tggcggggca caacttccgc gaagaccaga atcaggtgta
    42121 ccagccgttc gacctttcca acacgccgga agaccagcgt ttcctccaga gcaccggacc
    42181 tttcacgctg aagccgggtc agccggtgca gatcgtggtt gcgatgatgg tggcaaaagc
    42241 cgccggcaac ccgggtgatc ccatcagcgt gcgcgtggcg gagcttcagc gcgtttccga
    42301 cgttgcgcag tccattttcg acaacaactt cctccttccc gttccgccaa aggctcccgn
    42361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgcagtccat tttcgacaac
    42481 aacttcctcc ttcccgttcc gccaaaggct cccgccctga cggcgcgcgc gggagaccgc
    42541 accgcgtatc tctcatggga taccgttgcc gaaacgactc ccgacccgtt ctactcggtc
    42601 gcgtcggacc cggcgagtgc cctgtacgac cccaattaca agcggtacga cttcgagggc
    42661 taccgcgtat ggaagagcct gacaggccgt cccgatgact gggagctgct cgcggagtac
    42721 gacaggcaat cggtggtgcc gagaaacgcc gaaattacgg tgcaggcggg agtatatggc
    42781 ggaacactca cctatgacgc cgccacgacg gaaggctact tccttgagta tggcggaacc
    42841 agcctgttca acgggcatga gttccttgtc atgctggatg gcgccaccca gaaaatgaag
    42901 gtcttcgacg tcagcctcgg tgggcaggaa atcccctgca acacctatgc ggacggtgat
    42961 gactggtggt attaccatgg cgataactcg caattcgagt tgttcgattc aagtcttaac
    43021 tacctcagct cggattccac gaggaacgcc aacggcatca acgaccagta cgtgcccggt
    43081 atgatcatgt atctggccgg catggcgctc cagttcgata acgtgccggc tcctgccagc
    43141 accgtgatct ggcgcatcgt gccgtcgcag gaagaggact tcggtgccaa caccaccgtt
    43201 gcccacagct acatcgatcg ggacctgatc aacggacaga cgtactacta cgccgtgacg
    43261 gcgtatgact tccagctttc gtcgccgaga agcctcgaaa gtggacggac gctgagccag
    43321 gcggtggtag tgccgcgctc tgaacccaac ggtctggcga ctcctgatgt tgctccggtc
    43381 accaggatca gcgggtcgag cgacggtgtc gtgaacctcg aggtcgtgga tcccgtgtcg
    43441 gttacaggtc acacgtaccg gatcggattc aaggccgatg aaacgtggtt tgtgcgcgac
    43501 ctcagcaagc cgtctggtgc ggacacggtc gtgaacaacg tggccaacca ggcgggcggg
    43561 ttcgactacc cgattgtcga cggtgtgctg gtcaaagtca tcgggccgtc aatcggtatc
    43621 gaatccagca ggtacgtccc gagcgcaaac aaatggttcg tcgaacgtgc cgccgggtac
    43681 gaagtgatga cgggcgtaga ctggggttgg ggtaccacag taacgggcgc agactacgcg
    43741 cggattgagg tccgcttcaa cggcggttcg acgcgagcgc cggtgtacgt gcggggagca
    43801 agcccgaact acagctatca aggcgccggc acaatccctg cggaaatctg ggatgtgacc
    43861 aacaaccg
//
LOCUS       DAOSOO010000002        43500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_920_length_43500_cov_6.154747, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000002 DAOSOO010000000
VERSION     DAOSOO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 43500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43500
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_43500_cov_6.154747"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLT86_00175"
     CDS             complement(<1..629)
                     /locus_tag="PLT86_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT35063.1"
                     /translation="MIRWSSSKARWVAALACALATLYGTSLRAGTTGKIAGQVTDRAT
                     GQPIPGAAVLIEGLRLGSTTDADGRYFIIGVPPGSYSVKASLLGYAPITREGVIVNID
                     RTTPADFQLASSAIEVEALTVVAPREVIQLDVAGSRSTLAPEDVVALPNVTSLAGAIS
                     AEPGGNFRSGLAEETQLMLNGHMMTDDRVARYNASSIPITSVQEVQILTS"
     gene            complement(983..2914)
                     /locus_tag="PLT86_00180"
     CDS             complement(983..2914)
                     /locus_tag="PLT86_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35064.1"
                     /translation="MNSEPNSVARFTGRAVTAGAVISLLMAIGAPFSRYVLYSSPLVF
                     TSFSWGVVTTWLLFVVFEMGVLRRFRFWRPMSSPEFAVAFMIATVGSSLTTTEVAGPI
                     VTHIGGIHYFASPENRWHETLDSLLPVWLLPTDTHNAVKWLFDGVPQGQTYPWADWVV
                     PLFWHGSFTAAFLLVQFALVGLLRKHWVEHERLTFPIMQMAVELIQQPEEGEFRPPWS
                     RPRLFWFGFFIPLTFLTLQMLSWFWPTVPQLPTNLGAISFGTEYPALNMNLYWPVIGI
                     AFFANKEVIFSLLFFTVLGTLVTGWLTRLGIDVGVGAQPMQWLNMGALTVMVLWFLWL
                     ARRHLWSAARRAMGKPDGDDDSNEFISYRTAYLIIIGAVAYMVFWMQVSGSSIAIAFL
                     LIVMGTIVYLGIARLSFEAGVFHVNAPLRLVDMIVDATGSANISHASMAALAQHYWRF
                     SNVKSLFLVTFGHAGALSQQMEIPRRRLNPAIVIVTVATTLLATWYTLYLGVAYGGYN
                     FTDWIFRSGPADPFISLMKWISNPLPADFRRITFFGIGGGLMVLFSVLRYAFPWWPLH
                     PIGLAICFSFHIKESILSFLIAWVAKSLTLRLGGIKLFRRTVPLFVGIVVGAFVGTAM
                     CFLVDMIWFPMSGHAVEFK"
     gene            complement(3103..3360)
                     /locus_tag="PLT86_00185"
     CDS             complement(3103..3360)
                     /locus_tag="PLT86_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOT35065.1"
                     /translation="MPYVICEPCIGVKDTACVDVCPVDCIHPTKAEDAFETSEMLFIN
                     PEECIECGACEPACPVAAIYPEEDVPEEWREYIDRNAEYYR"
     gene            3682..5343
                     /locus_tag="PLT86_00190"
     CDS             3682..5343
                     /locus_tag="PLT86_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35066.1"
                     /translation="MVQSNPAESQPFALRGWGVRVWNNYDLFDAEVRKAAREAGRYHI
                     NLLDLHDGTVPPGIGWVELFAQYRHVQALRDKSSLTYKGTVIPERDREMYRQKFRELC
                     AFVKSQGLRIHVWYHVLRDLPEEWLIAEPTISRLEGRRLWQVIGSMLEDLFTGVPEID
                     ALVVTAGGTTAYPTRDQSSVTPGERLRAVYQCVYEACRRHRKQFIIRETGDTPSESDT
                     FLYAIGPLPPEIQIMVKNVQKDWCHLTAPVNPTLQRLGGKTVLLETDLYGEHWGRSAL
                     PLARPGYVFELVRSWLPLKAAGAVGRIMVQEEEDGVETSIFSSPGTVNLHAFGKVLAA
                     PGTTFQQAGTWLPEFDELDRGLWYEWAAQFGNKNGNVSPCLVSALETSPLICLYTLYL
                     AGAYFQDRSFFPGIETFEKSIWPTFVRKSKHIDLDVLLWEKDEAVRIAERSLEDLQRA
                     APTMDPQAVEVFTQFFEHAKDIAAAFRSLVELCAGRVKPETLRTTCRDANDLATRIEI
                     LRGPGFYGRLPERLRELSAYVQDHTTERSERADDAPPAEQEPTGP"
     gene            complement(5357..6010)
                     /locus_tag="PLT86_00195"
     CDS             complement(5357..6010)
                     /locus_tag="PLT86_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT35067.1"
                     /translation="METLSVSELFVSIQGESLAAGRLCAFVRLAGCNLRCVYCDTNYA
                     WDADAGRAMAVDDVAAWVSGSGVPLVEITGGEPLLQPAVYPLMETLIGRGLEVLLETN
                     GHVDARGVPERVTRIIDVKTPGSGMAEGDYLVNLPSLRENDQIKFVLTDRSDYDWAKE
                     VLAAHPELGRVSAILFSPAFETLPPRDLAGWIIADRLPVRFQLQLHKYVWGPEERGV"
     gene            complement(6059..7594)
                     /locus_tag="PLT86_00200"
     CDS             complement(6059..7594)
                     /locus_tag="PLT86_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT35068.1"
                     /translation="MKDRAGKSTDLVRPNVLLIMTDQQRADSLGCAGHPAVRTPHIDG
                     LAASGVRFDCAYTTHPTCTPSRGAIFTGRYPSSHGARMTGMILPESERMLPAVLREHG
                     YETALFGKPHFAPMGDYADCPSREMRHLWNDGAHPIEGDYYGFENVVFFGGHGPGIWG
                     HYRDWLRERDPAAERVIENASGDGGYMRAGAYEDGLADMFRSPLPAELHPTSALGEMV
                     SDYLRRRHEKPFFLVASFSDPHHPFAPPSEYADRYDPGDMPIPVPPDSDGFRTFPPHF
                     GSAYRGQSGMFSGGGHVDYSAIPPAAMLRARALTCGMVELIDAAVGNILRTLDETGLR
                     RSTVIVFCSDHGDFLGDHGFLYKGPMHWDGLTRVPFIWKLPGALRAGVVESGVASTLD
                     VTPTVLDLCGIPPEPGIEGISLAPYLRGQETYARDEALTENDDDYLGERLRTLVTAEW
                     KITAYAGRPWGELYDRVNDPHETRNLWDDRAFRGPRDEMRLRLLDHLMRREDRLPVQR
                     WYA"
     gene            7744..9627
                     /locus_tag="PLT86_00205"
     CDS             7744..9627
                     /locus_tag="PLT86_00205"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOT35069.1"
                     /translation="MHPLLLQLDLDHPGLEEANALARASRIHEALAAVVRYFRARPEP
                     DPSLLATPNPGTVEASERAIAREFVFYQVPGKVPPGNWDWTWRPGTDWEWTYALNRHH
                     WWAELAAAYLATGDQRYAQELDSLIRSWVGQCPVRVDDISSWRTLETGIRVAGPWIGI
                     LSAMKATPVITDTAWIMYLRAIHDHAEHLVAHPKANNWLLVESNGLLTCGIVFPEFRR
                     AKDWVRTAVERFEREMEQSVHPDGAHIEYSSSYHFLSLHSFTAAMDKADRTVGRVFSD
                     EFRNRLELMWEYPMYMMRPDGRLPLLNDSDTANIRPQLRAAGERFGRRDFVFAATNGR
                     EGTPPADTSHRFPWVRRAVMRSGWDEHAFYGFLETAPCGSGHVNEDALTFEIMAYGQP
                     LIGTMGRYSYDPVPVRRYLVSSAAYNTVMIDGQGQKWTKTHPDRSTWVAREPTADPWS
                     STAEQDVAYGRFNGPWTGDLSGVVWERRMAFHKPDAKLRRPGFWVVRDTFTGEGEHEL
                     RFLLHFYPGNVVCDDETHTVRSDYSGAQANVIVAFEHPEAVKFDCAYGQEDPPRGWYS
                     ARYGVIEPAWEVAVVRAAVFPADFVMVFVPYQAEAKLPVRVKREQGVVSVQLDDREWL
                     VSV"
     gene            9674..11287
                     /locus_tag="PLT86_00210"
     CDS             9674..11287
                     /locus_tag="PLT86_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35070.1"
                     /translation="MKLHNAFLVNGQPILGTYVASGVEELALCKEIGMNVVIGGHTEL
                     DVTTPEGAFCQQNGIKVMHHLVQHIYGKPRLADFVTPAQTEIPFVADLMKGVAQPSDE
                     LPKGVPSSGVFQIDDETVYYGKLTATHFTECVRGYRGTKPAEHRAGAFLFFPEACAEE
                     IRKVRTSPNLWGYYVLDDSPGDALSALRALYRTVKEEDPGRPVCAGYGSAGSLCNFGP
                     DVCDVMMFYWYPVAESWEPVAHGQYFRMLTPHQVQYMLMAAREMVPGIPFVGVYQSFD
                     AKFDPDQKNIAVPTADQIREQIEDYVREGACGLIAFLCSKKHFGFAHYPYMVEELKAI
                     HHEILETGELQVRPETEEMKRTRVQPVGFWEKPLPLPGVIPAWWTAFPFEDAKGEMLE
                     ATFGPDHEFDLDAVYPGKTWPVRWTVRRTFGGSLGFGELLGPHSYTKNCVAYAVCEVE
                     SPKQQEVILSIGSDEDALIKLNGKEVWRFGGGRGLTRDSDRVRVTLPQGKSRIFFKVR
                     NRQGLWGLDLRFLDGEGKPLEGLKFSPQP"
     gene            11373..11963
                     /gene="purN"
                     /locus_tag="PLT86_00215"
     CDS             11373..11963
                     /gene="purN"
                     /locus_tag="PLT86_00215"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT35071.1"
                     /translation="MNLGVLASGGGTNLQALIDRSEAGTLAAKVVVVISNNSTAGALE
                     RARRHGIPCYHLSRATCGDEASLDAAMCETMRRHGVDLVILAGYMRPVGPGLLQAYPD
                     RVLNIHPALLPKFGGKGMYGIHVHEAVLAAGDKTTGVTVHLVNSEYDRGRILAQRTAP
                     VLPGDTPESLQKRVLAIEHEIYADVINRIAAGEIRL"
     gene            12084..12989
                     /locus_tag="PLT86_00220"
     CDS             12084..12989
                     /locus_tag="PLT86_00220"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT35072.1"
                     /translation="MNSGLQETSLPGVPLFARGKVRDVYDLGERLLIVATDRISAFDC
                     VLPTPVPQKGVILTQMSLFWLSMMSDILPNHLITANVAEYPADLRKYGDQLEGRSMLV
                     RKARRIDVECVVRGYLAGSGWKDYQKTGMVCGHALPPGLRECDQLPEPIFTPATKEDS
                     GHDVNVSREHLVTLVGNELASELERLSLAIYERGREYAEKRGIIIADTKFEFGWIDGR
                     ISLIDEIMSPDSSRFWPADTYQPGRAQDSFDKQYVRDYLETLDWDKTPPAPPLPADIL
                     AGTQAKYREAYERLVGKPFRPFEGA"
     gene            12999..14642
                     /gene="purH"
                     /locus_tag="PLT86_00225"
     CDS             12999..14642
                     /gene="purH"
                     /locus_tag="PLT86_00225"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT35073.1"
                     /translation="MVKRALISVSDKTGIVPFAEALVSELGVEILSTGGTARALREKG
                     IAVTDVSSFTGSPEILDGRVKTLHPAVHGGILCRRDHPDDRKLIENGSIRPIDLVVVN
                     LYPFRETVAKPDVTIEDAIENIDIGGPTMIRSSAKNHAWVAVITDPKDYDSVLEELRK
                     TGGEISEKSRRRLAVKAFRHTAQYDAAIDTFLSERLEGEHVFHGHYVDGRVLRYGENP
                     HQTPAYVYRNPASDEPSVVSGKLLHGKEMSYNNYVDANSALAMVKDLAGSIAVSVIKH
                     NNPAGIATGETLAEALERAWYGDPLRTTPMGSVIAVTRPVDLAAAEFLKGRFVEVMVA
                     PGFEPDALEFLRGKSKDIRLVEIAPLATGTPEPFMAKTIAGGLLVQPVDRQVTATWNV
                     PTTAKFPPSLEPLAHFAVIAVKHTRSNAIILAREYRPGFYELVGLGAGQPNRVDSLRA
                     LAAPKARANFEMEYDEKRALNVKAGETRDEYVARQFSNVVLASDAFFPFDDSAVAAHS
                     WGIRYLVQPGGSIRDDEVVATCDKLGIAMALTGMRHFTH"
     gene            14700..15719
                     /locus_tag="PLT86_00230"
     CDS             14700..15719
                     /locus_tag="PLT86_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT35074.1"
                     /translation="MRFSLAGMISNDIGVDLGTANTLVWVKGRGIVLNEPSIVAVERN
                     TRKPLAIGAEAKQMLGRAPSEIEVIRPMKDGVIANFEIAEEMLRGFLTKVQKNRGLFR
                     PRVVICVPSGITEVEKRAVRDSAEAARANEVYLIAEPMAAAIGAGLPIENAVGSMIID
                     IGGGTSEIAVISLSGIVTTISERIGGDEMDEAIIQYVKRTYNLSIGNRMAEEIKIKIG
                     SAYPLPEEMEHTVRGRHLVTNIPQVVVVTASEIREALSESIDRIVQAVLQALERTPPE
                     LAADIIDRGIALSGGGALIKGLDERLREATNLPINVVDDPLTAVVRGTGIVLEDNSAY
                     KKVLM"
     gene            15770..16609
                     /gene="mreC"
                     /locus_tag="PLT86_00235"
     CDS             15770..16609
                     /gene="mreC"
                     /locus_tag="PLT86_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT35075.1"
                     /translation="MLQAFTHWVWAHIRGVTLTLTVVLSTALLIRGPQASIGPLRWGI
                     VHVIAAGQRVFGLPIELAHLRDENRYLRSLVLSHIIHQAENRELRSENERLRQLLNFR
                     ERSRFSLIAARVLAHDSEVPPTTITIDVGFVDGVGANMPVMTPEGLLGRVEGRPAGHR
                     SLVRLISNPSLRVAAVVENEDRGMGILRCDGKRLRVENVAQEARVREGDPVVTSGMGG
                     IFPPGIYIGKVLKVRNDPHALFKEMLISPGARLDRLEEVFVVREISPSAAIIGGVPDA
                     VDR"
     gene            16593..17093
                     /gene="mreD"
                     /locus_tag="PLT86_00240"
     CDS             16593..17093
                     /gene="mreD"
                     /locus_tag="PLT86_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOT35076.1"
                     /translation="MRWIVEALWLAAGAIAYATLGQEIALLGAVPILPLITIVRIAIT
                     SDELAGNLSGFGAGLLLDLFSLEWFGASMLVGSAVGYTLGVARKHLVIGNLATRGIIL
                     LVSAEVFALGVVLIKSARGAPGPEPFITALGSGIYTALVGIAWWFCVDLARGIFGWRG
                     LWDGAR"
     gene            17080..18909
                     /gene="mrdA"
                     /locus_tag="PLT86_00245"
     CDS             17080..18909
                     /gene="mrdA"
                     /locus_tag="PLT86_00245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT35077.1"
                     /translation="MVRADLSGMRSARIKVLMAVVLIAFLAITVQLYTLQVLRHDTLR
                     RRSEQNAMRTVPVEAIRGRITDRNGVVLVGSRPAYTVGVIPAEVKDAAHLDSMVCPIL
                     EIPPGTVSAKVSQQRQSLHRPIELRRDAPFQIIAYLEEHRHELPGIVYFVESRRRYPF
                     GILAAHLLGYVREVRESQLKQLKDEGYALGDLMGQAGVEATYERELRGEKGEEYQEVT
                     ATGRVVDSHRREPVRGMDARLSIDLALQQVAESAMDSIRRGALVALDPRTGEILALVS
                     RPAFNPDIFSTVVPPEIWRELNDDVERPLFNRATMGTYPPGSTAKMVSAAAGLETGVL
                     DSSTRLRPCTGGLAFGNRVYACWNKRGHGSLNVVHAIEASCNVFFYQVGERVGVDKWG
                     TYARLLGLGQKTGIDLPGEDEGVVPSTQVYDRRFGKGKWGRGEALNVGIGQGIVLVTP
                     VQMARYAAALATGYLVTPHLAIGFTDTNGVEHAPALRPPKPVSIQPDVLRLIRSAMVL
                     VTEGGSGTAHRAAVPGFHVAGKTGTAQNPHGDDHSWFVGFAPVPNPTIAIAVICENAG
                     HGSTRAAPVAGAVLRRHLLGPEQPRPKKDEEMTAGHPIAQLRN"
     gene            18915..20147
                     /gene="rodA"
                     /locus_tag="PLT86_00250"
     CDS             18915..20147
                     /gene="rodA"
                     /locus_tag="PLT86_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT35078.1"
                     /translation="MATSRQQLRKYLDWPLVGAALGLVACGIVGLLSATTGTATARVS
                     GLAGIQFLWLVLGIGVAIGAIRVPTRFWFGISYILYGISIIALVLTLAVGVSRMGAER
                     WLAIGPFRFQTSEMGKIGLILALARYLSFHKVSVSRFRDFAVTVGLVLVPIALIHRQP
                     DLGTALVYGSIFLPMVYWAGLANLHLLAIITPVINAVCAFAGWIPWLVFLLILVGVLF
                     LMRPPLRWTVVIAVINLVVGIVTPAIWDRLEPFQQRRIETFFSPEKDPLGAGYQIIQS
                     QVAIGSGGLWGKGFLEGSQTRLAYLPETHTDFIFAVLGEEFGFVGAIVIVALYVLLIW
                     RIFRMALVANNNFASFVCIGVGSMFAFHGIINIGMTIGLAPVTGLPLLLVSYGGSSLL
                     TNGLAIGMVLGFGVRRFE"
     gene            20170..21147
                     /locus_tag="PLT86_00255"
     CDS             20170..21147
                     /locus_tag="PLT86_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35079.1"
                     /translation="MKSGVRRLSLLFVVLPLGAVLMWSAWVVAAAGSSLRIFYTASVK
                     GYIDQCGCRYNPTGGIGRRATFIQRNTDGAIPMILLDGGDVIGEESPVERMQTTYLFR
                     AMKEMGYRTIGVGPRDFAYGIAYLREAERTYGFTFTCANLVDDATGRTLFAPHVVERV
                     SGKGGFRRNAATVRVGVVNVMGQDRLPLTVPSDPILRVTPPVPAVQNAVATLRPQTDV
                     IVVMVYGTQQLLDSVRAVPGVGAVLATRPLRLPDQWVSFNRTNVVGYSSFQGRGVGKI
                     DISLAGPHRITSGRGEMAMMTADIPDQPRMAELKREFEAWKERNAGGDR"
     gene            21114..22154
                     /locus_tag="PLT86_00260"
     CDS             21114..22154
                     /locus_tag="PLT86_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35080.1"
                     /translation="MEGTQRRGRQVAVRRVPGRWWAVLGVIAAIVVFFATLSLPQDAS
                     RLFIFFTATAHANLEPCGCPVNPSGGVSRRAAFIRSHTPPDTPVLLLDGGDVVGPPTP
                     EGLIRSEFMFRAMEEMGYSVLGLGARDFSFGPDFLRFAEKYYGFTFVGTNLLSSRTHA
                     HLFAPYAIARVGNGRILGIPFGGLRVGVLSVMGRDLIPFIPDTCEPAFLLDPLAAVRT
                     FSRRIRSQCDLVAVLAYTDQALLDSLVSMPGVDIVIAARTLNVPPGYTNVGMRGRAAL
                     AYTGYEARRIGVMRIRLDSSRNMTEARGDLITLSENLPEDETVRAMVQESRRAVAALP
                     NPGSLQKEPPAQ"
     gene            22190..23356
                     /locus_tag="PLT86_00265"
     CDS             22190..23356
                     /locus_tag="PLT86_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT35081.1"
                     /translation="MSTSSHGRLVALSVVLFTVFGAALTLTGPLLPQLIEHFGLSYGE
                     AGTLQVSSQVGYLAAIVLSGWLSAKIDGRMMLLAAIVFSLIGYGAFSLAPSFPAACAL
                     MIVGGAGGGAIEVIINAVVMKLSETRRSNLLNFIHLFFGVGAFIGPAVVGKLLALGVT
                     WHWFYFGTAVCWTALLLGTLACRFPSIKPVETASSEPARSVFSLPLARVLFLVLTLYV
                     GLELGVGGWVTTYLVHHEGMAPSYAAYSASAFWLGIAGGRLILGVIGHRWHESGILTI
                     LAAFSAACLLAAVLVPSPFVAALFLALTGLGYSGIYPTAVSFGGGAYPEQSTPVTALL
                     VTGGGIGAVAFPWLMGHISDGFGLRGGMLFYIVLNILLLGFILTARRMHSRAQT"
     gene            23375..23794
                     /locus_tag="PLT86_00270"
     CDS             23375..23794
                     /locus_tag="PLT86_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT35082.1"
                     /translation="MKSFRKELWFNIPSRRGFVNITKDVEQCVAESGVTEGLCLVNAM
                     HITASVFINDDEPGLHGDYEEWLEQIAPHEPISRYRHNRTGEDNGDAHLKRQIMGRET
                     VIAISRGKLDFGPWEQVFYGEFDGRRKKRVLVKIIGE"
     gene            23818..24588
                     /locus_tag="PLT86_00275"
     CDS             23818..24588
                     /locus_tag="PLT86_00275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOT35083.1"
                     /translation="MSTLDLFRLDGKVAIVTGAGRGLGREMAIALAEAGADVAITSRE
                     MAHVEPVAEEIGKLSGRRVLPVVADVTRPDDVQRMVDTTCRELGGLDILVNNAGVNIR
                     HLAHEYTMEEWNTIITTNVTGVFLCCQAVLPKMMAQKSGRIINIGSMMGVVGMPLRVP
                     YSASKGAVHQMTRTLSLEVAKYGITVNAIAPGPFLTEMNRQVIDQPDVYNFFVERIPL
                     GRWAEPNELRGVVVFLASQASNYITGSVIMVDGGWTAQ"
     gene            24654..25646
                     /locus_tag="PLT86_00280"
     CDS             24654..25646
                     /locus_tag="PLT86_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT35084.1"
                     /translation="MELRQLGKSDLKVTPVVFGAWAIGGWFWGGTDDAEAVTAIRAAV
                     DEGINTIDTAPMYGFGHSEEVVGKALKGIRDKVIVATKCGLRWNRTDGDFHFVAQDEQ
                     NRSHQIYKVLKPDSIIEECDLSLKRLNVDVIDLYQCHWPDTTTPIADTMGALMKLKEQ
                     GKIRAVGVSNFTPAMMRECLNAAPLASDQPKYSLLAREIEADVLPFCRENNIGVIVYS
                     PLAQGLLTGKVTMDRQFAPGDLRVNTPWFQPQNRQRVLDALGRIRPIADAHRATLGQV
                     AINCVINTPGVTSAIVGARNAAQVRENVHAAEFRLSEEEQSFIRSTFESLGKPV"
     gene            complement(25722..26021)
                     /locus_tag="PLT86_00285"
     CDS             complement(25722..26021)
                     /locus_tag="PLT86_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35085.1"
                     /translation="MVRSLLVTTLGLTGLVAATFPYQGISPARSGKVAAETEFQSGIV
                     LAEPAGDERHLPDLFVVASRENDGQFALTNAGKGADLPDLVVFGTRARSEDQPSS"
     gene            complement(26275..26634)
                     /locus_tag="PLT86_00290"
     CDS             complement(26275..26634)
                     /locus_tag="PLT86_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35086.1"
                     /translation="MKLHLIVLTVAAAALSIGALPAPKTTQSRTDSAPVETTLPDLLV
                     TAERTIAGGATGAVTKASQSGDLPDVVVVAEGPLSTAAPLEFRIQGGDLPDVTVFADR
                     PARTSAIAADHTVVTSF"
     gene            complement(26747..27586)
                     /locus_tag="PLT86_00295"
     CDS             complement(26747..27586)
                     /locus_tag="PLT86_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT35087.1"
                     /translation="MQDIRVAAVASLSRIGEIQTNLENIRQWSAKAKEQGAELVVFPE
                     LSVTGFWLHRDAHRYAQPVPGPATDTLVQIASEMGVLLVAGISEECQGRYYNTHVLVS
                     PSGILGTYRKTHINPYESPYYYEGNSLSVFDIGKARVGISICNDNLYPENLAVLALKG
                     AEILLMPFAYGGSNHNLWLASSACAAYRTRGLDLGCFVVAVNNAGPVPTPDGETNRYP
                     GGAFISDPEGEIVARTQGMGPGEKMLVADLRAKALHDRRSVPHFTLRLRRPELYGRVS
                     ELL"
     gene            complement(27602..29455)
                     /locus_tag="PLT86_00300"
     CDS             complement(27602..29455)
                     /locus_tag="PLT86_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35088.1"
                     /translation="MSDSRTNQPVDLAEVLLRSADPFFFVDDAGIIRAWNFGAELLLG
                     WTRDEALGAEVSIVLPEIPPGPPSEEEPEESLHDSVVAENFQTVFKAKDGHQIPVKIT
                     VTLIRDENGVLLGSAVNARNVAREKALEQELRWRIGEMEMLDNVARAVQSTLDIQRVL
                     RIILTAFTAGSGLGFNRAILFLVSGSLLEARLGIGSSNWEEAGRMWPRVADKPDLQSV
                     IDFVLAEDDTTPSVVHEIARDWRIPLEEAENPLIICMREKRSLIWPDSGDPGGSVAEK
                     LRSPMFAAVPLLHGGRAIGAVLADNVVTGRPIDEHAVRLLRLMAGSAASAISNARLYE
                     EVTRHAQNLEKASEQIKEQQDLLLQSRHFASLGRIVAAINHEVQGPLVPIGGFARALR
                     REVVDGSPQAEKLDFIIKEVARLENVVKGVASLAAVPLPTLHPVLLSALVEKVYTVVR
                     ADAGARGVILESDIPPDVPPPHIDHDQWYQALLVLVSKAVEVTPDGGTVSTSLRAEHG
                     RYVIRVSDSGPGIPQNQISKAFGPFFSTQPSLEAAGLNVVAEIVRRHFGRVSVQSSAT
                     GTTVAVDFPMADELYRLVEKQRADESNEDRIGLLDPLTQEVLSSASLANGN"
     gene            complement(29493..30155)
                     /locus_tag="PLT86_00305"
     CDS             complement(29493..30155)
                     /locus_tag="PLT86_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOT35089.1"
                     /translation="MDTNLLLTGLWHYGVALLALFPIVDPITAAFSFAGLTQNREPAW
                     TNRQALLACIYMVVTLIAFQILGTWILRFFAITLEAIRIAGGVMIWYAALEMLRGTDR
                     LSADEQIEGVAKHDIAFTPMAMPLLSGPGAIAVTLTLSARSERFVNEVAVGVAIITIA
                     VVTYLALRFASALQKHISETSRLAFSKVLGFLLLCVGVQFIMSGVAPLLGSGAFNAPT
                     GP"
     gene            30453..33560
                     /locus_tag="PLT86_00310"
     CDS             30453..33560
                     /locus_tag="PLT86_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT35090.1"
                     /translation="MLALGLDIGAISIKLAIVADREDADLVRGLVDKNDQYFLLEGVP
                     EGKVIVLSGYRRLFGSPRQSAEKMIAEVATSIPVDRVSFVGLTGIGAYPIAKTAGLAS
                     INEFRAVAQAVGYLHPEVETIFEMGGENSKFIRLARDRETGDVGIADYEKNGDCAAGT
                     GSFIDQQAARLKFSIEEVGGVVSGAGKPPTIAGRCSVFAKSDMIHAQQKGYQPPEILK
                     GLCEAVARNFKGAITKGKEIKPPIAFVGGVAANAGVVGAMRTVFELEEQDLFVPPAYA
                     WMTAIGAALLAPADEKGNRVEEFFRLRAKASGATHVLPTVQKLSMDRVISLRDRVKPC
                     TFEGKQLPVPVHLGIDIGSVSTNLVVIDDNGDLVYEIYTYTQSRPIQVVDAGLREIRD
                     AIGDKISVQSAGTTGSGRELIGLLIGADTINDEITAHKTGASYVGSRLINTKPDTIFE
                     IGGQDSKFISLNEGVVIDFTMNEACAAGTGSFLEEQAMKLGVQIKGEFARRALSAESP
                     TKMGERCTVFMEKDVTAYMQQGATVDEICAGLAYSVALNYLNRVVRGRPIGEHVFFQG
                     GTAYNDAVAAAFTQILDRPVIVPPHNGVIGAIGMALLAKDAVARKGERTTFRGWSLDA
                     INYELREFTCKGCSNHCDIQQFDVEGEKTYWGDKCSDRYRKSAKVNKKPVIEDLVARR
                     MEALLDGYHEPSVSGRVRVGIPRAMYFYDWFPFWKAYFTALDVDVVLSDTTSKQVINE
                     GTEAAVAEPCFPIKVAHGHVHNLLAKQVDFVWVPNMIDGETEFPETNSMLCPWGQTMP
                     FVFRAAPKFESLTGKWILPTLHPRRGEVVIERELWPVSQMFGASVGQHRRAVNAGYEA
                     SRRFRARLLALGQEALAILRAKDEMGIVLLGRSYNVNDPGTNLNVPTKLRTLYGTNVI
                     PMDCLPIVGIDIKDVNDNMYWNYGRKILQAARFVSQQPNLRVIYITNFKCGPDSYIKH
                     YTKDAAGGPFLTLQFDGHANDAGTLTRCEAYLDSQGFFTHEPRPIERPAQKALSRTRE
                     ERVEA"
     gene            33557..34852
                     /locus_tag="PLT86_00315"
     CDS             33557..34852
                     /locus_tag="PLT86_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35091.1"
                     /translation="MRSTGNPLAGRTLYIQQMTYASTHLLAAAFHSVGINAVVTEESD
                     ERTLELGGKFSSGEECLPEKITLGDYLKVVFSEGFDAKRAAFLMPTANGPCRFGQYSG
                     LLRKVLNDLGHHEALIFSPTSANGYDGFGANGNIFLRSAWRAVVVGDVLTKALLRTRP
                     YELRKGDTDEVFLRSIRRMGDVFSEKVESAGSQLERLVRALSVCHDEFCSLPVDMSEP
                     KPLVGVVGEIFCRLNDFSNDSIVRKLEEYGCETWLSDVAEWVWYTNDEAKTNMDADGL
                     LKIHPKRIKLWAKTTFQRHDEHALLSAFGDYFTGVEEASDVTEILNASRPYLPKEGSL
                     GEMVLSSGKAIYLYRMGADGIVDISPFTCMNGIVTEAIYTKLSKDCDGIPIRTFYFDG
                     ISSDLDRDVGIFVELVKTYRRRKKIARVWPHRKETTAVA"
     gene            35003..36061
                     /locus_tag="PLT86_00320"
     CDS             35003..36061
                     /locus_tag="PLT86_00320"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT35092.1"
                     /translation="MYKIAVIPGDGTGPEVVAEGLKCIAAVAGKFSFKYETTTFDFGG
                     ERYLRTGETLPDGALDELREFDAIYLGAIGHPDVKPGILEKGILLRTRFELDQYINLR
                     PVKLYPNVDCPLKDKGPADIDFVVIRENTEGLYTGSGGFLKKGMPDEVAIQTSINTRK
                     GVERCIRYAFEYTRRRNRRKTLTLCGKTNVLTFAFDLWERTFHEVGAEYPDIKREYAH
                     VDATTMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGVAAGGNINPEGVSMFEPI
                     GGSAPKYTGQGVINPIAAIGAAQMLLDTLGETKAAECLEKAIIEVVGTKMKSMSAGKM
                     GYSTSQVGDLVVEALLKS"
     gene            complement(36143..>36741)
                     /locus_tag="PLT86_00325"
     CDS             complement(36143..>36741)
                     /locus_tag="PLT86_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35093.1"
                     /translation="GRDSNIVKMIKILRGDSAQVDVQNPLAAVPPEAWAKLTPEQTQD
                     LIETSLALQRPSRHETIPSSDSRVEIIVPVAFFAACVLALFFLMRWRIHQSKLVHEQR
                     LAMIAQGIVPPDMATQPKPAAPRVQKMIIWGWIMALGGVGLSIANVLEDGWSEIGWGL
                     VIMLVGVGLLMAARYIGGQPVTGPKEGDVPPSQDSGTT"
     assembly_gap    36742..36841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(37130..37708)
                     /locus_tag="PLT86_00330"
     CDS             complement(37130..37708)
                     /locus_tag="PLT86_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35094.1"
                     /translation="MADQLKMRRRSNADVPEVALPSGYRLRHYVQGDEGVWADIINRA
                     GALGEYTEEKVVDTLTGQPRFHPEGLLFVTTDADEPVATACAWLGTHDDWRAGSVHMV
                     AVVPEHQGKRLSYWVCAAVLHVFRDWGVPDVYLTTDEFRTAAVKVYIQLDFEPVIRTP
                     EHYQRWCAVYRSYGMEERATALEKEAALGACW"
     gene            37769..39928
                     /locus_tag="PLT86_00335"
     CDS             37769..39928
                     /locus_tag="PLT86_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT35095.1"
                     /translation="MKAFWRNVALFTLVFLVVFTSIIAITVHLLSRDLPDLAAVEQIR
                     PKLSTVITSADGQILKTFAIQKRTLVAFDDLPPALTDALFASEDREFWSHWGVNPISF
                     VRASLISLVTLSPPRATSTLTQQLARDLFLTKERSLIRKIKEAIVATRIERTYTKKEI
                     IQLYLNQVYFGAGAYGVQAAARTYFGCDARDLTTEQCATLVGMLPAPNYYNPIRNPER
                     ALARRNVVLGAMGATRKLPKSEVARLQALPLVTTPGLEELGIAPYFTEYIRIRLSEQL
                     QKSDSLRIRFAQVAGLPDTASADQLIYEGGFVIETTLDSRLQQIAERVLFARIDSMQA
                     YYDRAVRAQNNINSPWVDRERSLATGSIVAKRVQAALLAMDVRTGAILAMVGGRDFDA
                     TKFNRAVQALRQPGSSFKPFVYTAAIDNGFSPVTEIPNQPVTLSDPQRGEWRPENYDG
                     SIGGPMTLREALAKSVNLVAIRLLLQTTPRLVIQYAQNMGIKSNLPPYPSLALGVGEV
                     RLVDLTAAYSVFPHQGIYVEPFAISRIISRDGIQLVERQISGRQHEALNPGTAFIMTK
                     LLENVVVRGTGAGARSQFGLLRPAGGKTGTTNDYGDAWFVGFTPQICCGVWVGFDQRV
                     NMGRGNTGAGAALPIWAQFMKEAHDVLNLPAQDFPKPPEIEAVEICDESHLAATPNCP
                     STYTEYFRSAEIPEPCTIHRWGRSSPASRREALRRNY"
     gene            complement(39922..40416)
                     /locus_tag="PLT86_00340"
     CDS             complement(39922..40416)
                     /locus_tag="PLT86_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT35096.1"
                     /translation="MNTQALVTEFVAYLRSRGMRMTPERRHILQHIFSAHTHFEVDDL
                     HVRLRDAGHRISKATVYRTVALLANCGILRSSIAPSGSASEYYELVYGLETPHEHLQC
                     QQCGRVFEVADPVLLGHLRRIAVTMGYELVDHSVKIVGRCVELENKGVCSKSGFQRPP
                     DKPQ"
     gene            40648..41769
                     /locus_tag="PLT86_00345"
     CDS             40648..41769
                     /locus_tag="PLT86_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT35097.1"
                     /translation="MECTRAAVVDYRALCEPPDFSGDAVAEQTRFPIAIEARVLQHLL
                     PREGVSPAWASDFGRILSDSEAIPVTTVDAVLVPDDTLTEDLRVSLEELDGIAQRWGT
                     VVAHLITAVPRKTLSSIVHQYLAAEDEQVLRFGQQVEKVLALPEDAGNLERCLAVIDH
                     LGAWVDRPAMCDIADVAYRLRMRIDEDRALLRQETERQIAEAPAKALDEFRAQTAVGF
                     CLPEQRMAFRGDTLFVFDGEYPAAEMHVLISEYMADLEKRFTAAAIDRDLLRQAAEAE
                     QPASIPDRVRSIVWRRDNGKCAMCGGTTDLEFDHIIPKSQGGASTVANVQVLCVRCNE
                     RKGAQVAWLPFPRAHRTSEDTLDLFRTPLPPRLERDSTC"
     gene            41821..42846
                     /locus_tag="PLT86_00350"
     CDS             41821..42846
                     /locus_tag="PLT86_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35098.1"
                     /translation="MPQYSVAIIGTGKAHRSDGATGYGTAHWHAQGYLAAPGCTLIAC
                     ADIVPENARAFAEMYNIPHVYVDYREMLSKEQPDIVSVCTWPQLHAQMVVDCANAGAK
                     AVHCEKPMAPTFGEARLMKDTCEKTGTQLTFNHQRRFEERFIFARDMIKAGAVGQLRR
                     LEGSWGNMLDVGTHWIDLLNFYNDDTPADWVIGQVYRAGDQKAFGVDVESQALCYFHC
                     ANDVYGLLLMGQGHSIGAEHRILGSEGIIEVDWPRVRIRTKGDASWQVFDKGATEGSV
                     TRGIQDVVASLSSGKESALSAKKAFAATEVIFATYESSRRRGRVELPLTIDDNPLHSM
                     IANGQIG"
     gene            42912..>43500
                     /locus_tag="PLT86_00355"
     CDS             42912..>43500
                     /locus_tag="PLT86_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35099.1"
                     /translation="MTELRAVIIGCGKSTRTAGAKEHGISHCHVQGYQATEGCKVTAC
                     ADIVEEYAASYAALYGLPKYYLDYREMIAAEKPDIVSVCTWPHLHAEMVIHCAKAGVR
                     AIHCEKPMAPTFGEARRMKETCDELGVQLTFNHQRRFEPQYRTVRELANRGVVGKVLR
                     IEAACGDMFDWGTHWFDMINFYNNDVPAEWIIGQVD"
BASE COUNT         9076 a        12399 c        13284 g         8641 t
ORIGIN      
        1 gacgtcagga tctgcacttc ctgcaccgag gtaatcggaa tgctggacgc attgtaccgc
       61 gcgacacgat catcggtcat catgtggccg ttcagcatga gctgcgtttc ctcggcgagg
      121 ccgcttcgga aattgccgcc cggttccgcc gaaatcgccc cggcgagcga ggtcacgttc
      181 ggcaacgcaa ccacgtcttc cggcgccagg gtggatcggc tgcccgcaac gtcaagctga
      241 atgacttcgc gcggcgcaac aacggtcagc gcttccactt caatcgctga cgacgcaagt
      301 tgaaaatcgg ccggcgtggt acgatcaata ttaacaatca ctccctcccg cgtaatggga
      361 gcgtaaccca ggagtgacgc cttcacggaa tacgagcccg gcggaacgcc gatgatgaag
      421 taccggccat cggcatctgt tgtcgaaccg aggcgcaacc cctcaatcag caccgcagct
      481 cccggaatcg gctgcccggt ggcacggtcg gtcacctgac ccgcgatttt gcctgtcgtg
      541 ccggcccgaa gcgaggtccc ataaagcgtc gcgagcgcgc acgcaagtgc ggcgacccat
      601 cttgccttgg acgatgacca gcgaatcatt gactctgcct cctgtcgaga ggtcaacaac
      661 taggccaaac agaggctgcc gcaccagagg cgtgacaacc cgtgcgccgt tcgaaaattc
      721 aaaaaagtcg tccgggaaac ttcacgaagt acgagaggag cggactgcgt atcccccgca
      781 tctcccttcc tcaaggtgac acgggcgtgc gtcatgcagc gcggacacgc agttgcagta
      841 cccattccga ccgccttccc tacggtgctg gatggtgctc tccccaagta atatatcccg
      901 acctcccatt tccatcaaga cggggggtcg ttaaagattc aacgcggcac acattcaatt
      961 tgccacgtaa gaagtatcgg acctatttaa actccaccgc gtgccctgac atcgggaacc
     1021 atatcatgtc aaccagaaaa cacattgccg tcccgacaaa cgcgcccacc acaatgccga
     1081 caaacagggg taccgtgcga cggaagagct tgattcctcc aagacgcagc gtaagagatt
     1141 tcgcaaccca tgcgatcagg aatgaaagga tactctcctt gatgtggaac gaaaaacaga
     1201 tggccagccc gattggatgc agaggccacc acggaaacgc gtaccgcaga acgctgaaaa
     1261 gcaccatcaa accgccaccg atgccgaaaa acgtgatgcg ccggaaatct gccggcaacg
     1321 ggttgctgat ccatttcatg agggaaatga acgggtctgc cggcccgctt cggaaaatcc
     1381 agtccgtgaa gttgtacccg ccgtacgcaa cgccaaggta gagcgtgtac cacgtcgcaa
     1441 gcagtgtcgt ggcaaccgtg acgatgacga ttgcgggatt cagtctgcga cgcggaatct
     1501 ccatctgctg agaaagtgct cccgcatggc caaacgtgac gagaaacagc gacttcacgt
     1561 tcgaaaaccg ccagtaatgc tgggcgagcg cggccatgct tgcgtgggat atattcgccg
     1621 agccggtggc atccacgatc atatctacga gccgcaacgg ggcgttgacg tggaacacgc
     1681 cggcttcgaa cgacagccgg gcaattccca agtagacgat cgtacccatg acgatgagca
     1741 agaaagcgat ggcgatgctc gaaccgctca cctgcatcca gaacaccatg taggccacgg
     1801 cgccgatgat gatgaggtag gccgtccggt aggatatgaa ctcgttggaa tcgtcgtctc
     1861 cgtccggctt gcccattgcg cggcgcgcgg cgctccacag atggcggcgc gccagccaga
     1921 ggaaccagag caccatcacc gtcagcgcac ccatgttgag ccactgcatc ggctgcgctc
     1981 ccacgcccac gtcgatcccg agacgcgtca accaccccgt aacgagcgtg cccagcaccg
     2041 tgaaaaacag cagactgaag atgacttcct tgttcgcgaa aaacgcgata ccgataaccg
     2101 gccagtagag attcatgttc aacgcagggt attcggtccc gaacgagatg gctcccagat
     2161 tcgtcgggag ctgtggaacc gtcggccaga accagctcag catctgcagg gtcaggaacg
     2221 tgagcgggat gaaaaacccg aaccagaaaa gccgtggcct gctccacggc gggcggaact
     2281 cgccctcctc aggctgctgt atcagctcca ccgccatctg catgatgggg aaggtgaggc
     2341 gctcatgctc cacccagtgc tttcgcagaa ggcccacaag cgcaaactga accagaagaa
     2401 acgcggctgt gaaactgcca tgccagaaca gcggaacaac ccagtccgcc cacggatacg
     2461 tctgcccctg cggaacgccg tcaaacagcc atttgaccgc attgtgggta tcggtcggca
     2521 gcaaccagac cggaaggagg gaatccagcg tttcatgcca ccggttctcc ggcgacgcga
     2581 aatagtggat tccaccgatg tgcgttacaa tcgggccggc aacttcagtg gtggtgagac
     2641 tcgaacccac ggtcgcaatc atgaaggcga ccgcgaactc cggcgaactc atgggtcgcc
     2701 agaaacgaaa tctcctcagg acacccattt cgaagacgac gaacagcagc catgtcgtca
     2761 ccacacccca ggaaaaagac gtgaacacga ggggtgagga atacagaacg tagcgcgaaa
     2821 acggcgctcc gatagccatc aggagggata tcaccgcacc ggccgtcact gcccgcccgg
     2881 taaaacgggc caccgagttg ggttctgaat tcatgagcaa ctcgccagcg taagggagaa
     2941 tctcgggctc accaggaaca ggtcacactc gttgcgcgct cgggttctgg tatgccgccg
     3001 gtgctggaat tctgaaaaac cggtggatta aaatttcaat gcttgcttcg gcgcgtcaat
     3061 ggggttgacc aaaacacacg caagaaaagt ctcacgcgcc gctcagcggt aatattccgc
     3121 gtttctgtca atgtactcgc gccattcctc cggaacatcc tcttccgggt agatcgcggc
     3181 caccggacag gccggttcgc acgcgccgca ttctatgcac tcctccggat tgatgaacag
     3241 catttcactc gtctcaaaag cgtcttctgc ttttgtcggg tgaatgcagt caacggggca
     3301 tacgtcgaca cacgccgtgt cttttacgcc gatgcacggc tcgcagatga cgtacggcat
     3361 tgccggacct cctgatgaag cgttgcggga aaaaacctgc cccccgggca ggggcgctgc
     3421 acccgcgcac agcacccacg ggtgattccc gggaaagacg atgcgaagtg tagcacggga
     3481 agacggtgct cgtcaacacg cccgccgtga atctcagaaa aagccccggg aacggctttg
     3541 tccggatgct gatgggccgc accgtatacc attgggttcc cggaaacctt acctttcttg
     3601 atatgcgttc tccggccgaa ccaccggccg caaccaaaaa cctgcgttcg agcaacagca
     3661 ccatttatcg gggacacatt catggttcaa agtaaccctg cggagtcaca accgtttgca
     3721 ctccgcggtt ggggtgttcg cgtctggaac aactacgatc ttttcgacgc ggaagtaagg
     3781 aaggccgcgc gggaagccgg ccgttaccac ataaatctgc ttgatctcca cgacggcaca
     3841 gtgcctccgg gaattggctg ggtggagctt tttgcccagt accgtcacgt ccaggcgctg
     3901 cgcgacaaga gctcccttac gtacaagggc acggtcattc ccgaacgtga tcgggagatg
     3961 taccggcaaa aattcaggga actctgtgcg tttgtcaaat cacagggcct gcgcattcat
     4021 gtctggtacc acgtcctgcg tgacctgcct gaagagtggc tgatcgcgga gccgacaatt
     4081 tcccgccttg aaggacgccg gctctggcag gtcatcggca gcatgcttga agaccttttc
     4141 acgggggtac cggaaatcga cgccctcgtc gtcactgcgg gcggcacaac ggcctatccc
     4201 acccgcgatc agagcagcgt taccccgggg gagcgtttgc gcgcggtata tcaatgtgtg
     4261 tatgaggcct gcagacgcca caggaaacag ttcattatcc gcgaaacggg cgatactccc
     4321 tcggaaagtg acacattttt gtacgcaatc gggccattgc ctccagagat ccagatcatg
     4381 gtcaaaaatg tgcagaagga ctggtgtcat ctgacggcac cggtaaatcc taccctgcag
     4441 cggcttggcg gcaagaccgt ccttctggaa actgacctgt acggcgaaca ctgggggcgt
     4501 tcagcattgc cgctggcacg acctggctac gtcttcgagc ttgtgcgctc atggttgcca
     4561 ctgaaggccg caggagcagt cggccgcatc atggttcagg aagaagaaga tggcgtcgaa
     4621 accagcatct tctcctcccc gggcacggta aacctgcatg cgttcggcaa agtgcttgcc
     4681 gcgccaggca caacgtttca gcaggccgga acatggttac ccgaattcga cgaactggat
     4741 cgcggcctct ggtacgagtg ggcagctcaa ttcggcaaca aaaacgggaa cgtcagtccc
     4801 tgcctcgtgt ccgcactgga aacctccccc ctcatatgcc tgtacaccct gtacctcgcc
     4861 ggcgcctatt tccaggaccg cagcttcttc cccggaatcg aaacgtttga gaaatcgatc
     4921 tggccgacgt ttgtccggaa atcaaagcac atagacctgg acgtcctcct ctgggagaaa
     4981 gacgaggcag tgcgaatcgc cgaacgatcg ctggaagacc tccagcgggc cgcccccacg
     5041 atggatccgc aggcggtgga agtgttcacc cagttcttcg aacatgccaa agacatcgcc
     5101 gcggcgttcc gttcgctcgt cgaactctgc gcgggacgcg tcaaaccgga aacactgcgg
     5161 accacctgcc gggacgccaa tgaccttgcg acacggatag aaatcctccg cgggccaggt
     5221 ttctacgggc ggttaccgga acgattgcgc gagctttccg cgtatgtcca ggatcacacg
     5281 acggagcgca gcgaacgcgc tgacgatgct cctcctgcgg aacaggaacc gacaggcccg
     5341 tgaagcttca cgctcactac acgccccgtt cctcaggccc ccacacgtac ttgtgcaact
     5401 gaagctggaa gcgcaccggc agccggtcgg caatgatcca tccggcgaga tcgcgcggcg
     5461 ggagagtctc gaacgcgggg ctgaacagaa tcgcgctcac tcgccccagt tcaggatgag
     5521 ccgcaagaac ttctttcgcc cagtcgtaat cactcctgtc cgtcagcacg aatttgatct
     5581 ggtcattttc ccgcagggac ggtagattca cgagatagtc cccctccgcc ataccgctgc
     5641 cgggcgtttt cacgtcgatg atccgtgtaa cccgttccgg gactccccgc gcgtccacgt
     5701 gtccgtttgt ctccagaagc acctccagcc cccggccaat gagcgtctcc atcaacgggt
     5761 agactgcggg ctgaagaagc ggctccccac ccgtaatttc caccagcggt actcccgatc
     5821 cgctcaccca cgccgcaacg tcgtcgacag ccatggcgcg acccgcgtct gcatcccatg
     5881 cgtagttcgt gtcgcaatag acgcaccgga ggttgcagcc ggcgaggcgg acgaatgcgc
     5941 acagccttcc cgcggcaagg ctttcaccct ggatgctgac gaacagctcg cttacggaga
     6001 gtgtttccat gtccctgtca cttctgatgt cgggcaggca gccggatggt aatcctcttc
     6061 acgcatacca tcgttgaacg ggcaacctgt cctctcgtcg catcaagtgg tcgagcaggc
     6121 gaaggcgcat ttcatctctc gggccccgga acgcccggtc atcccacaga ttccgtgttt
     6181 cgtgcggatc gttcacgcgg tcgtacagct ctccccacgg ccggccggcg tatgccgtga
     6241 tcttccactc cgcggtcacc agcgtacgca atcgctcacc gaggtaatcg tcgtcgttct
     6301 cggtcaatgc ctcgtcccgt gcatacgtct cctgcccgcg caggtacggc gccagcgata
     6361 tgccttcgat acccggttca ggaggtatcc cgcacagatc caggacggtc ggcgtcacgt
     6421 caagcgtcga agcaacgccc gattcgacga cacccgcccg caaggcaccc ggcagcttcc
     6481 agatgaatgg cacacgcgtt aacccatccc agtgcatggg ccctttataa aggaatccat
     6541 gatcgccaag gaagtccccg tggtccgagc aaaaaacgat aaccgtactc cgccgcagcc
     6601 cggtctcgtc gagcgtccgc aggatgttcc ccactgcagc gtcgatcagt tccaccattc
     6661 cgcatgtgag ggcgcgggca cggagcattg cagcaggcgg gatggccgag taatcaacgt
     6721 ggccgccgcc gctgaacatc ccgctctgcc cccggtacgc gctgccaaaa tgcggcggaa
     6781 acgtcctgaa tccatccgaa tccggcggta caggaatcgg catgtcaccg ggatcgtacc
     6841 ggtcggcgta ttcagatggc ggggcgaacg gatgatgcgg atccgagaag gatgcaacca
     6901 ggaaaaacgg tttctcgtgc ctgcgtcgca ggtagtccga taccatctcg ccgagggcgg
     6961 aagtgggatg gagctctgcc ggcagcggtg aacggaacat gtcagccagc ccgtcttcgt
     7021 atgccccggc ccgcatgtac ccgccgtcgc cggaagcgtt ttcgatcaca cgttccgcgg
     7081 cgggatcacg ttcgcgcagc cagtcccggt aatgccccca gatgcccgga ccatgccccc
     7141 cgaagaagac cacgttctca aatccgtagt aatcgccttc aatcgggtgt gccccgtcgt
     7201 tccagaggtg tcgcatttcg cggctcggac agtccgcgta gtcgcccatc ggggcaaaat
     7261 gcggcttgcc gaacagcgcg gtttcgtagc cgtgctcccg gagaacggcg ggcaacatgc
     7321 gctcgctctc cggaagaatc atcccggtca tgcgagcccc gtgggagctg gggtaccgcc
     7381 ccgtgaaaat cgcacctcgg gacggcgtac acgtgggatg agtcgtatac gcacaatcga
     7441 accggacgcc ggatgccgcc agtccgtcaa tgtgcggggt gcggaccgca gggtgcccgg
     7501 cgcacccgag gctgtccgca cgctgttgat cggtcatgat gagcagtacg ttcgggcgca
     7561 ccagatcggt gctttttccc gcgcggtctt tcacctcagg tctcctttcc gtccacgtcc
     7621 aagggaatgt aatcacccgc cgattcgcct ccaattcgca cgagtgtggt ttgtccgctg
     7681 accggccgtg gctacattgc gagaagggga aaacccgctc tttttgttgc cgcggagata
     7741 cgcatgcatc ctcttttgct ccagctcgac cttgaccacc cgggactgga agaggcgaac
     7801 gcgcttgcca gagcatctcg catccacgag gcgctggccg ccgtagtccg ttacttccgt
     7861 gcgcgtcctg aacccgatcc gtccctcctc gccacaccga accccggtac agtggaggcc
     7921 tctgagagag ccatcgcacg ggagttcgtg ttctaccagg tccccgggaa ggtgccgccc
     7981 ggcaactggg attggacgtg gcgcccggga accgactggg agtggaccta cgcgctcaac
     8041 cggcaccact ggtgggcgga actcgcggct gcgtatctcg caaccggcga ccaacgttac
     8101 gcgcaagaac ttgattcgct gatccggagc tgggtggggc aatgtccggt tcgcgtggat
     8161 gacatctcgt cctggcggac acttgaaacc ggaattcgcg tcgcggggcc atggatcgga
     8221 attctctccg cgatgaaggc gactcccgtc atcaccgata ccgcatggat catgtacctg
     8281 cgggccattc acgaccacgc ggagcatctc gtcgcgcacc cgaaggcgaa caactggctt
     8341 ctcgtggaat ccaacgggtt gctcacctgc ggtatcgtgt tccccgaatt cagacgtgca
     8401 aaagactggg ttcgtaccgc ggtggaacga tttgagcggg aaatggagca gagcgtgcac
     8461 ccggacggag cccacatcga gtacagctcc agctaccact tcctttcgct ccacagcttc
     8521 acggcagcca tggataaggc agacagaacg gtcggccgtg tcttttccga cgagttccgg
     8581 aaccggctgg aattgatgtg ggaatacccg atgtacatga tgcgccccga cgggcgactc
     8641 cctcttttga acgacagcga cacggcgaac atccgtcccc agcttcgggc cgccggcgaa
     8701 cgtttcgggc gacgggattt tgtgttcgcg gcgaccaacg gccgggaagg cactcccccg
     8761 gcggacacga gccaccggtt cccgtgggtg cgccgcgcag tcatgcggag cgggtgggac
     8821 gaacacgcat tctacgggtt cctcgaaacg gctccgtgcg gctcgggaca cgtcaacgag
     8881 gatgcgctca cattcgagat catggcgtac ggccagccac tcatcggaac catgggacgg
     8941 tattcgtacg atccggtgcc ggtccgcaga tatctcgtca gcagcgcagc atacaacacc
     9001 gtgatgatcg atggtcaggg ccagaaatgg acgaaaacgc accccgaccg ttccacctgg
     9061 gtcgcccgtg aaccgaccgc agacccttgg tccagcaccg cggagcagga tgtcgcgtac
     9121 ggccgtttca atggcccgtg gacgggtgat ttgtcgggtg ttgtctggga gcggcgcatg
     9181 gcgttccaca aacccgacgc gaagcttcga cgccccgggt tctgggttgt gcgggatacc
     9241 ttcacgggag aaggcgagca cgaactgcgc ttcctgttgc atttttaccc cggaaatgtc
     9301 gtgtgcgacg acgaaacaca tacggtgcga tctgactact ccggggcaca ggcgaacgtt
     9361 atcgtcgcgt ttgaacaccc cgaggctgtg aagttcgatt gcgcgtacgg tcaggaagac
     9421 cctccacgag gctggtacag cgctcgatac ggcgttatcg agccagcgtg ggaggtggca
     9481 gtcgtgcgcg ccgccgtctt tcccgcggat ttcgtcatgg tcttcgtgcc gtatcaggct
     9541 gaggcaaaac ttcctgttcg tgtcaagagg gaacagggtg tcgtgagcgt acaactcgat
     9601 gacagggaat ggctggtgtc cgtctagaag gacttcccgg cgaaccaaac ttttttttgg
     9661 aggacctccc ggtatgaaat tgcacaacgc gtttctcgtc aacgggcaac cgattctcgg
     9721 cacgtacgtc gcttccggag tggaagagct tgcgctgtgc aaagaaatcg gcatgaacgt
     9781 ggtgatagga ggccacaccg aactcgacgt gacaaccccg gagggagcct tctgccagca
     9841 aaacggcatc aaagtcatgc accacctcgt ccagcacatc tacggcaaac cccgcctcgc
     9901 ggatttcgtg acgcccgccc agacggaaat cccgtttgtc gccgatctca tgaaaggtgt
     9961 cgcacaacca tcggatgagc ttcccaaagg cgttccgtct tccggagtgt tccagatcga
    10021 cgatgaaacc gtgtactacg ggaagctcac tgcaacgcac ttcacggagt gtgttcgcgg
    10081 gtacagggga acgaaaccgg cggagcatcg tgccggggcg ttccttttct tcccggaagc
    10141 atgcgcggaa gagatccgga aggtccgcac ctcaccgaat ctgtggggct actacgttct
    10201 tgatgacagc cccggcgatg cactctcggc gctcagagca ctgtaccgga cagtcaaaga
    10261 ggaagatccg ggacgtccgg tctgtgccgg atacggaagc gccggctcac tgtgcaattt
    10321 tggaccggat gtgtgcgacg tgatgatgtt ctactggtac cccgtcgcgg aaagctggga
    10381 accggtggcc cacggacagt acttccgcat gctgaccccg caccaggtgc aatacatgct
    10441 gatggcagcg cgggagatgg ttcccgggat tccgttcgtg ggcgtgtatc agtcctttga
    10501 tgcgaaattc gaccctgacc aaaagaatat cgccgtcccg acggcggacc agattcgcga
    10561 acagatcgag gattacgttc gggaaggagc gtgcggcctg atcgcgttct tgtgctcgaa
    10621 aaagcatttc gggttcgcgc actaccccta catggtggag gaactgaaag ccatccacca
    10681 cgagatactg gaaacaggcg agttgcaggt ccggccggag accgaagaga tgaaacgaac
    10741 gcgtgtccaa ccagtcggtt tctgggagaa gcctctgcca ttgccgggtg tcatacctgc
    10801 ctggtggacg gcattcccgt tcgaagacgc aaaaggcgag atgctggagg caaccttcgg
    10861 cccggaccac gaattcgacc ttgacgcagt gtaccccggg aaaacgtggc cggttcggtg
    10921 gaccgtacgc agaaccttcg gaggctctct gggcttcggt gaactcctgg gccctcattc
    10981 gtatacgaaa aactgcgtcg cgtacgcagt gtgcgaggta gaaagcccga aacagcagga
    11041 agtcattctt tccatcggaa gcgacgagga cgcgctgatc aaattgaacg gcaaggaagt
    11101 gtggcggttt gggggtgggc gggggctcac gcgcgatagc gacagagtgc gtgtcacatt
    11161 gccgcagggc aagtctcgaa ttttcttcaa ggtgcggaac agacagggcc tctgggggct
    11221 cgatcttcgt tttctggatg gcgagggtaa gccgctcgaa gggctgaagt tttcgccaca
    11281 accgtaacga gtcttccggc ggaacggcaa ccacgcgggt tccgttccgc gtctctctga
    11341 atcgcggcat ttcatggagg tgtgatcagt tgttgaacct gggagtcctg gcatccggcg
    11401 gcggaacgaa ccttcaggcg ctcatagacc gaagcgaggc aggcacactg gcggcgaagg
    11461 tggtcgtagt catcagcaac aactcgactg caggagcgct ggagcgggct cgcaggcatg
    11521 gcatcccctg ttaccacctc agcagggcaa catgcggcga tgaagcctcg ctggatgcag
    11581 ccatgtgtga aacaatgcgg cgccacgggg ttgatctcgt cattcttgcg gggtacatgc
    11641 gccctgtcgg ccccggcctc cttcaggcgt atcccgaccg cgtgctgaac atccacccgg
    11701 cactgcttcc aaaattcggc gggaagggga tgtacggcat acacgttcat gaagcggttc
    11761 tggcggcagg cgacaagaca accggcgtta ctgtccacct cgtcaattcg gagtacgaca
    11821 ggggcagaat actcgctcaa cgaaccgcgc ccgttctgcc cggtgacacg ccggaatcgc
    11881 tccagaaacg cgtccttgcg atcgaacatg agatctacgc ggacgttatc aacagaatcg
    11941 ccgcgggcga aatccgcctc tgacggcgga agcggagcgg cttctcacgt ttcggaccga
    12001 ttgcattttt tcgtgcatgc gttgtacttt gtgaaagtct ggctccaatc ggttcgttta
    12061 cccccttttt catgagagcg gatatgaact ccggactgca agagacgtcc ctgccgggcg
    12121 tgccgttgtt cgcgcggggc aaagtgcggg acgtgtacga tctcggcgaa agattgctca
    12181 tcgtcgcgac cgacagaatc agcgcgttcg actgcgtcct gccaacgccg gttccgcaga
    12241 aaggcgtcat tctgacgcag atgtcgttat tctggctttc catgatgagc gacatactcc
    12301 cgaaccacct catcaccgca aacgtggccg aataccccgc ggatttgcgc aaatacgggg
    12361 accaactgga aggtcggtcc atgctggttc gcaaagccag aaggattgac gtcgagtgcg
    12421 tggttcgagg atatctcgca ggatcaggct ggaaagatta ccagaaaacc ggaatggtgt
    12481 gcggacacgc attgcctccg ggtctgcggg aatgtgatca gcttccggaa ccgatcttta
    12541 cgccggcgac aaaagaagat tcaggacacg atgtgaacgt cagtcgcgag cacctcgtga
    12601 cactcgtcgg aaacgagctg gcatcggaac ttgaacggct gagccttgcg atctacgaac
    12661 gaggccggga atatgccgaa aaacgcggca tcatcatcgc cgatacgaaa ttcgaattcg
    12721 ggtggattga cgggcgtatc agcctgattg acgagatcat gtcccccgat tcgtcccgtt
    12781 tctggccggc ggatacctat cagccgggtc gggcgcagga cagcttcgac aagcaatacg
    12841 tgcgagacta tctggaaacc ctcgactggg acaagacgcc tcctgcgcca ccgctgcccg
    12901 ccgacatact tgccggcacc caggcaaaat accgcgaagc atacgagcgt ctcgtaggaa
    12961 aaccatttcg tccttttgaa ggagcgtagc acatcgtgat tgtcaaacgt gcattgatca
    13021 gcgtttcaga caagacaggc atcgtgcctt ttgctgaagc gctggtttcc gaactcggcg
    13081 tcgaaatact ctccacgggc ggcaccgcca gggcccttcg cgagaaaggt atcgcggtaa
    13141 ccgacgtctc ctcgttcacc ggcagcccgg agattctcga cggccgcgta aaaacgcttc
    13201 accccgcggt ccacggaggg attctctgcc ggcgggatca tcccgacgac cggaagctca
    13261 tcgagaatgg gtccattcgc ccgatagacc tggtggtcgt aaacctctac cccttccgcg
    13321 aaaccgtcgc gaaaccggat gtcacgattg aagatgcgat agaaaacatt gacatcggcg
    13381 gtccgacgat gatccgttca tcggcaaaga accacgcatg ggtcgccgtg ataaccgatc
    13441 cgaaggatta cgattccgta ttggaggaac ttcggaaaac cgggggagag atttcggaga
    13501 agtcgcgccg gaggctggcg gtcaaagcgt tccgccacac cgcgcagtac gatgcggcaa
    13561 tcgacacctt tctcagcgag cggctggaag gggagcacgt cttccacggt cattacgtgg
    13621 acggcagagt gcttcgatac ggcgaaaatc cccatcagac gcctgcgtat gtctaccgta
    13681 accccgcaag cgacgaacct tccgtggttt ccggaaaact cctccacggc aaggagatgt
    13741 cctacaacaa ctacgtcgac gccaattcag cgcttgcgat ggtgaaggat ctcgcgggtt
    13801 ccatcgccgt ttccgtgatc aagcacaaca acccggccgg tatcgccacg ggagaaaccc
    13861 tcgccgaggc tctggaacgg gcatggtatg gcgacccgct gcgcaccacc cccatgggaa
    13921 gcgtgattgc ggtgacgcga ccggtggatc tggccgcggc cgaattcctc aagggccggt
    13981 ttgtcgaagt gatggtggca ccggggtttg agcctgatgc cctcgagttt ctccgcggta
    14041 aaagcaagga tatccgcctt gtggaaatcg cgccgctcgc cacgggcacg cccgaaccgt
    14101 tcatggcaaa aaccatcgcc ggagggttgc tcgtccaacc cgtggacagg caggtaaccg
    14161 caacgtggaa cgtgccgacc accgcgaaat tcccgccctc gctggagcca ctcgcccatt
    14221 tcgcggtgat tgccgtcaaa cacactcgat caaatgccat catccttgcg cgtgagtatc
    14281 gccctgggtt ctacgaactg gtgggactcg gagcgggcca gccgaaccgt gtggacagtc
    14341 tccgtgcgct ggcggcacca aaagccaggg cgaatttcga gatggagtac gacgaaaaac
    14401 gcgctctgaa tgtgaaagca ggcgaaacgc gggacgaata cgtcgcgcgg cagttcagca
    14461 atgtcgttct cgcgtcagac gcgttctttc cgtttgacga ctcagcagtt gccgcacatt
    14521 cctggggaat ccgctatctt gttcagccgg gcgggtccat ccgcgatgac gaagtcgtcg
    14581 ccacgtgcga caaactgggc atcgcgatgg cgctgaccgg aatgcggcac ttcacgcact
    14641 gaaccaatcg aacgaaacgg gacaacacac acgccacagc accagcgtcg aggagtgcca
    14701 tgcgattctc tctcgccggg atgatttcaa acgacatcgg ggttgatctc ggcactgcga
    14761 acacccttgt ctgggtaaaa ggccgcggca tagtgctcaa cgagccgtcc atcgttgcgg
    14821 tcgagcgaaa caccaggaaa ccgcttgcca tcggcgcgga agccaagcag atgctgggtc
    14881 gtgcaccgtc ggaaatcgaa gtgattcgtc cgatgaagga tggcgtcatc gcgaatttcg
    14941 agatcgcaga ggaaatgctc cgcggcttcc tgacaaaagt gcagaagaat cgggggcttt
    15001 tccggccacg ggtggtgatc tgtgttccgt cgggtatcac cgaggttgag aaacgtgcgg
    15061 tgcgcgacag cgccgaggcc gcccgggcga atgaggtgta cctcattgcc gagccgatgg
    15121 ccgccgcgat cggtgcgggg cttcccatcg agaacgccgt aggctcgatg atcatagata
    15181 tcggcggggg aacaagtgag atcgccgtga tcagcctttc gggtatcgtg acgacgattt
    15241 ccgagcgcat tggcggcgac gagatggacg aggcaatcat ccagtacgta aagcgcacgt
    15301 acaacctgtc catcggcaat cgcatggcgg aggagatcaa gatcaagatc ggctcggcgt
    15361 atccactgcc cgaggaaatg gagcacacgg ttcgcggccg ccatcttgta acgaatatcc
    15421 cgcaggttgt tgtcgtgacg gcttccgaga tccgggaagc gctttcggag agcattgatc
    15481 ggatcgtgca ggcggtgctt caggctcttg aacgcactcc gccggaactt gcggctgaca
    15541 tcatcgacag gggaatagcc ctgagcggcg gcggcgcgct gatcaagggg ctggacgaac
    15601 gtttgcgaga agccaccaac ctccccatca acgtggttga cgacccgctc accgcagtcg
    15661 tgcgggggac gggcatagtg ctggaggaca attcggccta caaaaaagtg ctcatgtagg
    15721 aaacagccgt gccgacaggt gcacgaccaa ggtaaggtgt tgataccgta tgctgcaggc
    15781 attcacgcat tgggtgtggg cgcacatccg cggcgtcacg ctcacgctta cagtagtcct
    15841 ttccaccgcg ctgctcatac ggggaccaca ggcctcgatt ggacccctcc ggtggggaat
    15901 tgttcacgtt atcgcggcag ggcagagagt tttcggcctc cctattgaac tggcacacct
    15961 tcgagatgag aaccgctacc tgcgaagcct cgttctctct cacatcatcc atcaagcgga
    16021 aaaccgggaa ctccgaagcg aaaacgagag gctgcggcaa ttgctgaatt tccgtgaacg
    16081 cagccggttc tctctcattg cggcccgcgt acttgcacac gattcggagg ttccgcccac
    16141 cacgataacg attgatgttg gctttgtgga cggcgtaggt gccaatatgc cggtgatgac
    16201 accggagggg ttactcggcc gcgtcgaggg tcgtccggcc gggcatcgtt cgttggtgcg
    16261 gctgatctcg aacccgagcc tcagggttgc ggccgttgtg gagaatgaag atcgcggaat
    16321 gggcatactt cggtgcgacg ggaagcgatt acgcgtggaa aacgtcgcgc aggaggcacg
    16381 ggttcgagag ggggacccgg tcgttacgtc cgggatgggc ggcatattcc ctccgggcat
    16441 ttacatcggg aaggtcctca aggttcgcaa cgatccgcac gcgttgttca aagagatgct
    16501 gatttccccc ggagcgcgtc tcgacaggct ggaggaagtc tttgtggtgc gcgagatctc
    16561 gccttccgcg gcgataatcg gtggggtgcc ggatgcggtg gatcgttgag gcattgtggc
    16621 tggcggcggg agccatcgcg tacgccaccc tcgggcagga aatcgcgctg ctcggcgcgg
    16681 tgccgattct cccgctgatt acgatcgttc gaatcgcaat cacctcagac gagcttgcgg
    16741 gtaacctctc cggtttcggc gcaggactcc ttctggacct tttctcgctg gaatggtttg
    16801 gcgcatccat gcttgtcgga agtgcggtcg ggtatacact cggcgtagca cgaaagcacc
    16861 tcgtcatcgg caatctggca acccgtggga ttattctcct cgtgtcggcg gaagtattcg
    16921 cgcttggagt ggtgttgatc aagagcgccc ggggtgctcc cggtcccgaa ccgttcatca
    16981 ctgcgcttgg ttccgggatt tacaccgcac tcgtcggcat cgcatggtgg ttctgcgtgg
    17041 atcttgcgcg cgggattttc gggtggcggg ggctgtggga tggtgcgcgc tgacctctcc
    17101 ggcatgcgaa gtgcgagaat caaggttctg atggcggtgg ttctcatcgc atttctggca
    17161 attaccgtcc agctctacac actccaggtc ctgcgccacg atacactccg caggcgttcc
    17221 gaacaaaacg cgatgcggac cgttcccgtt gaggcaatcc gtgggaggat tacggatcgc
    17281 aacggggtgg ttctcgtcgg gagccggccg gcgtacacgg tgggagtgat cccggctgaa
    17341 gtgaaggacg ccgcgcatct tgacagcatg gtatgcccga tcctggagat tccaccggga
    17401 acggtttccg cgaaggtgag ccaacaacgt cagagccttc atcgccccat tgagttgcgc
    17461 cgggatgctc cgtttcagat aatcgcctac ctcgaagagc accggcatga gttgcccggt
    17521 atcgtgtatt tcgtcgagtc gcgcagaagg tatccgttcg ggatacttgc cgcccacctg
    17581 ctggggtacg tgcgggaagt ccgcgaatcc cagttgaagc agttgaagga tgaggggtac
    17641 gcgctgggcg acctcatggg ccaggcaggt gttgaagcca cctacgaacg ggagctccgg
    17701 ggcgaaaagg gtgaggagta ccaggaagtg actgccaccg gccgcgtcgt agacagtcat
    17761 cggcgtgaac cggtgcgcgg catggacgcg aggctcagca ttgacctggc tcttcagcag
    17821 gtcgccgaat ccgcgatgga ctcgatacgg cggggcgctc tcgttgcgct ggaccccaga
    17881 accggggaaa tcctcgcgct ggtgagcagg cctgcattca acccggacat attctcgacc
    17941 gtcgttccac ctgagatatg gcgggagctg aacgatgacg tcgagcgtcc cctgttcaac
    18001 cgtgcaacga tgggaacgta tccaccgggg agcacggcga agatggtcag cgcggcggcg
    18061 ggtctggaga ccggcgttct ggacagctcc acccggttgc ggccctgtac cgggggactg
    18121 gctttcggaa atcgtgtgta cgcatgctgg aacaagagag ggcatggctc actgaacgtt
    18181 gtgcacgcga tagaagcctc ctgcaatgtc tttttctatc aggtgggcga aagggtgggt
    18241 gtcgacaagt ggggaacgta cgcacgattg ctcggactgg gacagaagac cgggatagat
    18301 ctgccgggag aggatgaggg cgttgtgccg tccacgcagg tatatgatcg aaggttcggg
    18361 aaagggaaat gggggcgggg agaggcgctg aacgtgggta tcggccaggg gattgtgctt
    18421 gtgaccccag ttcagatggc acggtacgcc gccgcgcttg caacgggtta tcttgtcacc
    18481 ccacatcttg cgatcggttt taccgacacg aacggtgtgg aacatgcgcc tgcgttgcga
    18541 cccccgaaac cggtttctat ccagcccgat gtgttacgtt tgattcgcag cgccatggtc
    18601 ctcgtaaccg agggaggcag cggaaccgct caccgggccg ccgtgccggg ttttcacgtc
    18661 gcggggaaaa cggggaccgc gcagaacccc cacggggatg atcactcgtg gttcgtcggt
    18721 ttcgcaccgg ttccaaaccc cacgatcgcc attgcggtga tttgtgagaa tgcggggcat
    18781 ggaagcacga gggcagcacc cgtggccggg gcggtacttc ggaggcacct gctcgggccc
    18841 gaacagcctc gccccaagaa ggacgaggaa atgactgccg ggcatcctat cgctcagttg
    18901 aggaattgac cacgatggcg acgtcgcgac aacagctcag gaagtacctc gactggccgc
    18961 ttgtcggcgc ggcgcttggt ctggtcgcgt gcgggattgt cgggctcttg agcgcgacga
    19021 cgggtaccgc caccgcccgc gtcagcgggc ttgcgggcat ccagtttcta tggctggtcc
    19081 tggggatcgg cgtggcgatc ggggcgatcc gcgtgccgac gcgcttctgg ttcgggattt
    19141 cctacatcct gtacgggatc agcattatcg cgctggttct tacccttgcg gtcggtgttt
    19201 cgcgcatggg ggcggagcgg tggctcgcca taggtccctt ccgctttcag acgtcggaga
    19261 tggggaagat cgggttgatc cttgcgctgg cccgttacct ctcgtttcac aaggtgtcgg
    19321 tttcccgttt tcgcgacttc gcggtcaccg tcggactggt tctggtgccg atcgctctta
    19381 ttcatcgcca gccggacctc gggaccgcgc ttgtctacgg atccattttt cttcccatgg
    19441 tctactgggc cggtctggca aaccttcatc tgctcgcaat aatcaccccg gtgataaacg
    19501 cagtgtgcgc ctttgcgggg tggatcccct ggctggtatt cctgctgatt ctggtcggag
    19561 tactcttcct catgcggcca cctctccgct ggacagtagt gattgcggtg atcaatctgg
    19621 tcgtgggcat tgtcacgccg gcaatctggg accgcctcga accttttcag cagcgacgga
    19681 ttgagacatt tttcagcccc gagaaagatc cgctgggcgc gggctatcag attatccaat
    19741 ctcaggtggc gattggttca ggagggttgt ggggcaaggg attcctcgaa ggttcccaaa
    19801 cgcggcttgc gtatctgccg gagacccaca cggatttcat ttttgcggtg cttggagagg
    19861 aattcggttt cgtgggcgcg attgtcatcg tagcgcttta cgttctgctg atctggcgca
    19921 ttttccggat ggcacttgtt gccaacaaca acttcgcgtc gtttgtttgt attggcgtgg
    19981 gttcgatgtt cgcgttccac ggaataatca atatcggtat gacgatcggg cttgcgcctg
    20041 taaccggtct tccgttgctg ctggtgagtt acggcggctc gtcgttgctg acaaacgggc
    20101 ttgcgatcgg tatggtgctc ggtttcgggg tgcggcggtt tgagtaaaca ggacacggag
    20161 aaggatgcca tgaagagtgg ggtgcggcgc ttgtcgcttt tgttcgtggt gttgcccctt
    20221 ggtgcagtgc tgatgtggag cgcatgggtt gtggccgcgg cggggtcgtc actcaggatt
    20281 ttctacacgg cgagtgtcaa agggtacatc gaccagtgcg gctgccggta caaccccacc
    20341 ggcggcatcg gacggagagc aacgttcatc cagaggaaca cggacggcgc cattccgatg
    20401 attcttcttg acggtggcga tgtcatcggc gaggagagcc ccgttgaacg catgcagacg
    20461 acgtatcttt tccgggcgat gaaggaaatg gggtaccgga cgatcggtgt cggcccccgg
    20521 gattttgcgt acggcatcgc gtatctgcgt gaggccgaac ggacgtacgg gttcaccttc
    20581 acctgtgcca accttgtgga cgacgccacg gggcgcaccc tgttcgcgcc ccatgtagtg
    20641 gagcgcgttt ccgggaaggg cgggttccgc cgaaatgccg caacggtgag ggtcggtgtg
    20701 gtgaatgtga tggggcagga tcgccttccg ctcacggtac ccagcgaccc gattcttcgc
    20761 gtcactccgc cggttcccgc ggttcagaac gccgttgcca cgctgcggcc acagacggat
    20821 gtgatcgtgg taatggtata cggcacgcag cagttgctcg acagcgtgcg cgccgttccc
    20881 ggggttggcg cggtgctggc aacgcgtccg ctgcgtcttc ccgatcagtg ggtatcgttc
    20941 aatcgcacga acgttgtcgg gtactcctcg ttccagggaa gaggcgtcgg aaagattgac
    21001 atctcgcttg ccggacccca ccggattacc tccgggcgag gcgagatggc gatgatgacc
    21061 gcggacatac ccgaccaacc ccgtatggcc gagttgaagc gggagttcga agcgtggaag
    21121 gaacgcaacg ccgggggaga caggtagcgg tgcgtcgcgt tccagggcgt tggtgggctg
    21181 ttctcggggt catcgcggcg atcgtggtgt tttttgcgac gctttcgctg cctcaggacg
    21241 cctcccggtt gttcatcttc ttcacggcca ccgctcacgc gaatcttgag ccgtgcgggt
    21301 gcccggtgaa tccgagcggg ggagtttccc gccgcgctgc atttatccgg tcgcacactc
    21361 ctcccgacac gccggtgctg cttctggacg ggggagacgt ggttggcccc ccaacgcctg
    21421 agggattgat cagatcggag ttcatgttcc gcgcgatgga ggaaatgggt tattcggttc
    21481 ttggtcttgg cgcgcgggat ttctcattcg gccccgattt tcttcgtttt gccgagaagt
    21541 actacggctt cacgttcgtc ggaacgaatc tgctttcttc ccgcacacac gctcacctgt
    21601 tcgccccgta cgcgattgcg cgggtgggca atggaaggat tctgggaatt ccgttcggtg
    21661 gcctgcgggt cggagtgctc agcgtgatgg gaagggattt aatcccgttt attccggaca
    21721 cgtgcgagcc agcattcctt ctggatccgc tggcggctgt tcgaacgttt tcgcggcgca
    21781 ttcgttcgca gtgtgatctc gtcgcggtgc tcgcgtacac ggaccaggct ctcctcgact
    21841 cactcgtgag catgcctgga gtagacatcg tcatcgcagc gcggacgctt aacgtacctc
    21901 ccgggtacac aaatgtcggt atgagaggaa gagccgccct tgcctacacc gggtacgaag
    21961 ctcgtcgaat cggagtaatg cgtattcgtc ttgactcgtc gagaaacatg actgaggcgc
    22021 gcggtgactt gattacgctc agcgagaacc ttcctgagga tgaaacggtc agagcgatgg
    22081 tacaggaatc ccgccgcgcc gttgcagccc ttcccaaccc cggttccctg cagaaagaac
    22141 cacccgcgca gtgacgcggg tccgttatcc agccagaagg agtacctgca tgagcacctc
    22201 gtcccacggg cgccttgttg ccctttccgt ggttttgttc actgttttcg gtgcggcgct
    22261 cacgctcacc ggccctcttc tcccgcaact catcgagcac ttcggattat cgtacggaga
    22321 agccggaacg ttgcaggtat cgtcgcaagt cgggtatctg gctgcgatcg tcctatcggg
    22381 gtggctttcc gcgaaaattg acgggagaat gatgttgctt gcagcaattg tgttctccct
    22441 tatcggttac ggtgcgttca gccttgcgcc gtcctttccg gcggcctgcg cgttgatgat
    22501 cgtcggcggg gcgggcggcg gcgcaatcga ggtcatcatc aatgccgtgg tgatgaaact
    22561 gagcgaaacc cggcggagca atctactgaa tttcattcac ctgttcttcg gagtgggggc
    22621 attcataggc cccgccgtgg ttggcaagct tctcgcgctc ggcgtgacgt ggcactggtt
    22681 ctatttcggg actgcagtgt gctggacggc tctgcttctg ggaacactgg cgtgccgttt
    22741 cccttccata aagccggtgg aaacggcgtc ttcagagccc gcccgcagcg tgttctctct
    22801 tcctctggcc cgcgtgttgt tcctcgtcct tacgttgtac gtgggcctgg aacttggagt
    22861 tggagggtgg gtgacgacgt atctggtcca ccacgagggc atggctccgt cgtacgccgc
    22921 ctattcggcg tcggcattct ggctcgggat tgccggagga cgtctcatac tcggcgtaat
    22981 tggtcaccgc tggcatgaat cggggatact gaccatactg gcggcgtttt cggcagcgtg
    23041 cctgctggcc gcggtcctgg ttccgagccc cttcgtcgcg gcgctgttcc ttgccctgac
    23101 cggtctcgga tattccggaa tctacccgac ggccgtttcc ttcggcggtg gcgcgtatcc
    23161 ggagcaatcc acaccggtga ctgctcttct tgtcaccggc gggggcattg gcgcggtggc
    23221 attcccgtgg ctgatggggc acatttccga cgggttcggg ttgcgggggg ggatgctgtt
    23281 ctacatcgtt ctgaacatcc tcttactcgg gttcatcctt accgcgcggc ggatgcacag
    23341 tcgggcccag acgtaatgag cgggagaaat tgagatgaaa agcttccgga aggagctgtg
    23401 gttcaacatt ccaagccgtc gcgggtttgt gaacatcaca aaggacgttg agcaatgcgt
    23461 ggcggaaagc ggggtgaccg aagggctatg cctcgtgaac gcgatgcata tcaccgcgag
    23521 cgtgttcata aacgacgatg agccggggct tcacggcgac tacgaggagt ggctggaaca
    23581 gattgcgccc cacgaaccga tttcgcgtta ccggcacaac cgaacgggcg aggacaatgg
    23641 cgacgcacac ctgaaacggc agatcatggg gcgtgagacg gtcatcgcga tttcgcgcgg
    23701 aaagcttgat ttcgggccct gggagcaggt gttctacggg gaattcgacg ggcggcgcaa
    23761 gaaacgcgtg ctggtgaaga taatcggcga atagcgaaac tgagaaaggg agagtacatg
    23821 agcacactgg atttgtttcg tctggacggg aaggttgcca ttgttacggg tgccggacgc
    23881 gggctcggga gggaaatggc gattgctctt gcagaagcgg gagcggatgt ggcgatcacg
    23941 agcagggaaa tggcacacgt ggaaccggtt gctgaagaga tcgggaagct ttcgggcaga
    24001 cgagtgcttc ctgttgtggc cgacgtgacc aggcctgacg acgtgcagcg gatggtcgat
    24061 acgacgtgca gggaactcgg tggtctggac attctcgtga acaatgccgg ggtgaacatc
    24121 cgccatctcg cccacgagta tacgatggag gaatggaaca ccatcatcac gacaaacgtg
    24181 accggtgtct tcctttgctg ccaggcagtg ctcccgaaaa tgatggctca gaaatcggga
    24241 cggatcatca atatcggctc gatgatgggt gtcgtcggta tgccgttgcg ggtgccgtat
    24301 tcggcgtcga aaggggccgt gcatcagatg acgcgcacgc tttcacttga agtggcgaag
    24361 tacggtatta cggtcaacgc gatagcgccg gggccatttc ttactgagat gaaccggcag
    24421 gtgattgacc agccggacgt gtacaacttt tttgttgaac ggatacctct cggcagatgg
    24481 gcggagccaa atgagctgag gggagtggtg gtgttccttg cctcgcaggc atcgaactac
    24541 attacgggaa gcgtcatcat ggtagacggc ggctggaccg cgcagtagct gaaaactccg
    24601 cgcggtatgc cgggtcagtc aactggttgg ttggaacctg aaggaggccg gacatggaac
    24661 tccgtcaact tgggaaaagc gatctgaagg ttacgcccgt cgtgttcggg gcgtgggcaa
    24721 tcggggggtg gttctggggt ggcacagacg acgcggaggc ggtgacggcc atccgcgccg
    24781 cggttgacga gggcatcaac accatagata ccgcgccgat gtacggtttc ggccacagcg
    24841 aagaggtcgt gggaaaagcg ctgaaaggga tcagggacaa ggttatcgtg gcgaccaaat
    24901 gcgggctgag atggaaccgc acggatggtg attttcattt tgtcgcgcag gacgagcaga
    24961 accgatcgca ccagatatac aaggtgctga agcctgattc gatcatcgag gagtgtgatt
    25021 tgagtttgaa acgcctgaac gtcgacgtta ttgaccttta ccagtgccac tggccggata
    25081 caactacgcc gattgccgac acgatgggcg cgttgatgaa gctgaaagag cagggcaaaa
    25141 tccgggcagt tggagtgagc aacttcactc cagcgatgat gcgcgaatgc ctcaacgcgg
    25201 cacctctcgc cagcgaccag ccgaagtaca gcctgctggc gcgggaaatc gaggcggacg
    25261 tgctgccctt ctgccgtgaa aacaatatcg gcgtgatcgt gtacagcccg cttgcccagg
    25321 ggcttctaac gggcaaagtg acgatggatc ggcagttcgc tccgggcgat cttcgggtga
    25381 atacgccctg gtttcaaccg caaaaccgcc agcgcgtgct ggacgcgctg ggaagaatcc
    25441 gccctattgc ggacgcgcac cgagccacgt tggggcaggt tgcgatcaac tgtgtgatca
    25501 atacgccggg tgttacgtcg gcaatcgttg gcgcgcgcaa cgccgcccag gtgcgcgaaa
    25561 acgttcacgc ggcggaattc aggctttccg aagaggagca gtcgttcata cggagcacat
    25621 tcgaaagcct gggaaaaccg gtttgacggg ttcaccgtga aaaagaaccg gggtggcgcg
    25681 gacataccgt gccaccccgt tcttttcgtg gaccgttcac gtcatgagct cggttgatct
    25741 tcactccgtg ctctcgtgcc gaacacgacc agatccggga ggtcggctcc ctttccggca
    25801 ttcgtcagcg cgaactgacc atcgttctca cgagaggcaa ccacaaacaa atcgggaagg
    25861 tgtctctcgt ctccggccgg ttcagcgagg acaatcccgg actggaactc ggtttccgcg
    25921 gcaacttttc ccgatcgagc gggggagatc ccctggtacg ggaacgtcgc ggcaaccagc
    25981 ccggtgaggc cgagtgtagt aacaagaagt gatcgtacca tcttcgtgta tctccttcaa
    26041 tccaagaaat ccagttattg aagcgacttt gcagattatc cgccggggct tttgacccct
    26101 gcacaagaat agacaacgtg accgcattgt ttgttgcaac cagagcgctc ggaggagtcg
    26161 aactgcacga ggagcagtct gaactgcaga taccggcatt gcagtgcacg ccgaaaaaaa
    26221 agcgcggacg ccagggatca cctggcgtcc gccttcacgg gtgtccggct tcttttagaa
    26281 gcttgtcacc acggtatggt ctgccgcgat tgccgaggtg cgggccggtc ggtccgcgaa
    26341 gacggttaca tccgggagat cgccgccctg gatgcggaat tccagaggtg ccgccgtgct
    26401 caaggggcct tcggcaacca caaccacgtc cggcagatca cccgactgac tggccttcgt
    26461 tacggcgccc gtagccccgc cggcgattgt cctttcggcc gttaccagga ggtccggcag
    26521 tgttgtttcc accggagcac tgtctgttct gctttgcgtg gtctttgggg ccggtaacgc
    26581 tccgattgaa agagccgctg cggccaccgt caggacgatc aggtgaagct tcatcttgta
    26641 ttcctcccat gttgcctttt cctgttccgt tcgcttcgtt cactggatca gacgagagga
    26701 agacgagatt tgttacactt tccgacgtgc ctggccgaaa aagaacttag aggagttcgc
    26761 tgacacgtcc gtaaagctcg ggcctgcgga gtcgcagcgt aaaatgggga actgaccggc
    26821 ggtcgtgaag cgcttttgcg cgcaggtccg caacgagcat tttttctccc ggtcccatac
    26881 cctgtgttct ggcgacgatt tctccttcgg ggtcgctgat aaaggctcct ccgggatatc
    26941 tgtttgtctc accgtcgggc gtgggaacgg ggccggcgtt gttcacagca acgacgaagc
    27001 agcccagatc gagcccgcgc gttcgatacg cagcgcatgc ggaactcgcg agccacagat
    27061 tatggttgct gcccccgtac gcgaacggca tcagaagaat ctccgctcct ttgagcgcca
    27121 gaactgccag gttctccgga tacaggttgt cattgcagat ggagatgccg actcgcgctt
    27181 tgccgatgtc gaacaccgaa aggctgttgc cttcgtagta gtagggtgat tcgtaagggt
    27241 taatgtgcgt tttgcgatag gttccgagga ttcctgacgg cgaaaccagg acgtgtgtgt
    27301 tgtagtacct tccctggcat tcctctgaga ttcccgcaac cagcagcacg cccatttcgg
    27361 aggctatctg caccagtgta tcggtcgcag gaccgggtac cggctgcgcg tacctatgcg
    27421 cgtccctgtg aagccagaaa ccggtgactg aaagctcggg gaatacgacg agttcagctc
    27481 cctgttcttt cgcttttgcc gaccattgac ggatgttttc caggttcgtc tggatttcgc
    27541 cgatgcggct caacgaggct actgctgcga ccctgatatc ctgcatgtaa tccgtccttt
    27601 ctcaattccc attcgccagg gacgcggacg acagcacctc ctgcgtcaac gggtcgagaa
    27661 gaccgatccg gtcctcgttg ctctcatcgg cgcgctgctt ttccacaagc cggtacagct
    27721 cgtccgccat cgggaaatca accgcgaccg tggtgccggt tgctgagctc tggaccgaga
    27781 cgcgtccaaa gtgccgacga acgatttccg cgacgacgtt gagtcccgcg gcttcgaggc
    27841 tcggctgggt gctgaaaaaa ggtccgaagg ctttcgaaat ctgattctgg gggatgccag
    27901 ggccggaatc ggacacgcgg atgacatacc tgccgtgttc ggctctcaga cttgtgctca
    27961 cagtgccgcc gtccggggtt acctccaccg cttttgagac gagcacgagg agtgcctggt
    28021 accactggtc atggtcgatg tgcgggggag ggacgtcggg cggaatatcg ctttcgagga
    28081 tcactccccg cgcaccggcg tcagcccgga ccaccgtgta taccttctcc accagagcgc
    28141 tgagcagcac gggatgcaat gttggcagcg gaaccgccgc gagcgacgca acgcctttca
    28201 ccacgttctc aaggcgagcg acttccttga tgatgaaatc cagtttctcc gcctggggag
    28261 agccatccac tacctcacgc ctcaaggcgc gggcgaaacc accgatcgga acaagggggc
    28321 cctgcacttc gtggttgatc gccgccacga tgcggccgag agacgcaaaa tgccgggact
    28381 gaagaaggag gtcctgttgt tctttgatct gctcgctggc cttttccagg ttctgcgcgt
    28441 gccgcgtgac ttcctcatac aaccgcgcat tcgaaatggc ggatgcggcg cttcccgcca
    28501 tgagtcgtag cagacgcacg gcgtgttcgt caatcgggcg accggtgacc acattatccg
    28561 ccagcaccgc accgatggca cgccctccgt gcaggagagg aaccgcagcg aacatggggc
    28621 ttcgcagttt ttcagccacg gagccgccgg gatctcctga atcaggccag atcagcgacc
    28681 tcttttctcg catgcatatg atgagggggt tctctgcttc ttcaagaggg atgcgccagt
    28741 cgcgtgcaat ttcgtgaaca actgaggggg tggtgtcgtc ctcagcaagc acgaagtcga
    28801 tgacggattg aaggtcgggt ttgtccgcga cacggggcca catgcgcccg gcttcttccc
    28861 agttggaaga tcctatgccg aggcgtgctt cgagcagact ccccgagaca aggaaaagta
    28921 tcgcgcgatt gaaaccgagg ccgcttcccg cggtgaaggc ggtgagtatt attcgaagca
    28981 cccgctggat atcgagcgtg ctctgcacgg cgcgggcaac gttgtcaagc atttccattt
    29041 cgccgatgcg ccagcgaagt tcctgttcga gcgctttttc ccgggcgaca ttccgcgcat
    29101 tcactgcact cccgagcaga accccatttt cgtctctgat gagggtgaca gtgattttca
    29161 ccggtatctg gtgaccgtct ttcgctttga acactgtctg gaaattctcg gcgaccacgg
    29221 aatcgtgaag ggattcctca ggctcctcct cggaaggcgg accgggtggg atttccggca
    29281 acacgatgct cacttcagcg ccgagagcct cgtccctcgt ccaaccgagg agcagttccg
    29341 ccccaaaatt ccaggcgcgg atgatgccgg cgtcgtcaac aaagaagaat ggatcggcag
    29401 accggaggag gacttcggcg agatcgaccg gctggttcgt tcgggaatcg gacatctctc
    29461 tgagttactc tccgccgtgc attcagttcg cgttacggcc ctgtcggggc gttgaaggcc
    29521 cctgatccga gcagcggcgc gacgcccgac atgataaact ggactccgac gcaaagcagc
    29581 agaaaaccga gcactttcga gaacgcgagg cggcttgttt cgctgatatg cttttgaagg
    29641 gccgatgcaa accgaagagc gaggtacgtc acaactgcta tcgtgatgat ggctactccg
    29701 accgcgactt cgttgacgaa tcgctcagag cgcgcgctca gtgtcagtgt taccgcgatg
    29761 gcaccagggc ccgacaggag cggcatcgcc atgggcgtaa acgcgatgtc gtgttttgcg
    29821 acaccttcga tctgttcgtc ggcggagagc ctgtctgttc cgcgaagcat ctcaagtgcc
    29881 gcgtaccaaa tcatcacacc gccggcgatt cggattgctt ccagagtgat ggcgaagaag
    29941 cgaagaatcc aggtccccag gatttgaaac gcgatgagtg tcactaccat gtaaatgcac
    30001 gccagaaggg cctgacggtt agtccacgca ggttccctgt tctgggtgag tccggcgaaa
    30061 ctgaaggcgg cggtaatggg atccacgatg ggaaaaagcg cgaggagagc gacgccgtag
    30121 tgccagaggc cggtgagaag aaggttcgtg tccatttttc caactggttt gcagtcggcc
    30181 cggagagccg cacacgcata taagggaaga tacacgcacg caggcgttcg tccaaaactg
    30241 cgtgtgagaa ggtggctttc tggcgaacgg attgctgcgc gtgattaatt gcaattgatt
    30301 ctcaatatga aatgccgccc cgtcgttgcc cccgcacttc gcgcacaaaa acccgcaact
    30361 cgcctgtact gtgcttccgc ttcgctgcat gggccggttg aaatggagaa cttgtacggt
    30421 cgcgtaaggc cgttcgagga aaggacacgt agatgcttgc actgggactt gatatcggcg
    30481 cgatcagtat caaactcgcg attgtcgccg accgtgaaga cgccgatctg gtgcggggac
    30541 tggttgacaa gaacgaccag tatttcctgc tggaaggtgt tccggaaggg aaggtgatcg
    30601 ttctgagcgg gtaccggcgg ttgttcggaa gtccacggca atccgcagag aaaatgatcg
    30661 ctgaggtggc caccagtatt cccgtagaca gggtttcctt cgttggcctg acgggaatcg
    30721 gcgcgtatcc gatcgcgaag acagccggcc ttgcgtccat taacgaattc cgcgcggttg
    30781 cgcaagcagt tggatatctc caccccgagg ttgagacgat tttcgaaatg ggcggagaga
    30841 actcgaaatt catccggctt gcgcgggacc gtgagaccgg cgacgttgga attgccgact
    30901 acgaaaaaaa cggtgactgc gccgcgggca ccgggtcgtt cattgaccag caggctgcgc
    30961 gtctcaagtt ctccatcgag gaagtgggcg gcgtggtctc gggtgcgggc aagccgccaa
    31021 cgatcgccgg cagatgctct gtgttcgcga aatctgacat gatccacgcc cagcagaagg
    31081 ggtaccaacc gccagaaatc ctgaaaggtc tctgtgaggc ggtcgcaagg aacttcaaag
    31141 gggcaatcac gaaggggaaa gagatcaagc cgcccatcgc gtttgtcggg ggtgttgccg
    31201 caaatgcggg cgtggttggc gccatgcgca ccgtgtttga gctggaagag caagatcttt
    31261 tcgttcctcc cgcatacgcc tggatgacgg ccatcggcgc cgccttactg gcaccggccg
    31321 acgaaaaagg gaaccgggtg gaggagttct tccgacttcg cgccaaggct tcgggggcga
    31381 cacacgtcct tccgacggtg caaaaactga gcatggacag ggtgatttcg ctgcgagatc
    31441 gcgtgaagcc gtgcacattc gaaggcaagc agttgccggt tccggtacat ctgggaattg
    31501 acatcggttc cgtcagcacc aacctggtgg taatcgacga caacggggac ctggtgtacg
    31561 agatttacac gtacacccaa tcccggccta ttcaggtagt ggatgcgggg ctcagggaaa
    31621 tccgcgacgc gatcggagac aagatcagcg tccaatccgc aggaaccacg ggaagcggca
    31681 gggaactgat cgggcttttg ataggcgcag acacgatcaa cgacgaaatc accgcgcaca
    31741 aaaccggagc aagctatgtc ggaagtcgcc tgatcaacac caaaccggac acgattttcg
    31801 agattggcgg gcaggattcc aagttcatca gtctgaatga aggtgttgtc atcgacttca
    31861 cgatgaatga ggcgtgtgcg gcaggaaccg ggagttttct tgaagaacag gccatgaaac
    31921 tcggtgtcca gataaaaggg gagtttgcgc gccgagccct gtcggcggaa tctccgacaa
    31981 agatgggcga gcgctgcacg gtgttcatgg aaaaggacgt taccgcctac atgcagcagg
    32041 gcgccaccgt ggatgaaatc tgcgccgggc ttgcgtattc cgtggctctc aactacttga
    32101 accgcgtcgt gcgcgggagg ccgatagggg aacacgtgtt tttccagggg ggaacggcgt
    32161 acaacgacgc agttgcggcg gcgttcacgc aaatcctcga cagacctgta attgttccgc
    32221 ctcacaacgg cgttatcggc gccatcggga tggcccttct ggcgaaggac gcggtcgcgc
    32281 gcaaagggga acgaaccacg ttccgcggat ggagcctgga cgcgatcaac tacgagcttc
    32341 gggagttcac gtgcaaaggc tgttcgaacc actgtgacat acagcagttt gacgtggaag
    32401 gagaaaaaac ctactgggga gacaagtgct cggaccgtta ccggaagagc gcgaaggtga
    32461 acaagaagcc ggtgatcgaa gacctggtag cgcggcggat ggaggcgttg cttgacgggt
    32521 atcacgagcc ctcggtgagc ggccgtgtac gcgtgggtat tcccagggca atgtacttct
    32581 acgactggtt cccgttctgg aaggcctatt ttaccgctct tgacgtggat gtggtgctga
    32641 gcgacaccac ctcgaagcag gtcatcaacg agggcacaga agcggccgtc gcggagccct
    32701 gtttccccat aaaagtggcg cacggacatg tgcataacct gctggcgaag caggtggatt
    32761 tcgtctgggt gcccaacatg atcgacgggg agacggaatt cccggagacg aattccatgc
    32821 tgtgcccgtg gggacagacg atgccgttcg tattccgcgc ggcaccgaag ttcgagtccc
    32881 ttacgggcaa gtggattctg ccgacactgc acccgcggcg gggcgaggtg gtaatcgagc
    32941 gggagttgtg gcctgtttcc cagatgtttg gtgcatcggt ggggcagcat cgtcgcgcgg
    33001 tgaatgcggg ttacgaggcc agccgccgct ttcgcgcgcg gttgctcgcg ctggggcagg
    33061 aggcgctggc aatcctgcgc gcaaaggacg agatgggaat cgtgctgctc ggacgatcgt
    33121 acaacgtcaa tgatcccggg actaatctga acgtcccgac gaagcttcgc acgttgtacg
    33181 gtacgaatgt catacccatg gattgtcttc cgatcgtggg catcgacatc aaggatgtga
    33241 acgacaacat gtactggaat tacggtcgca aaatcctcca ggcggcacgg ttcgtttccc
    33301 agcagccgaa tctgcgggtt atctacatca cgaatttcaa atgcgggcct gattcgtaca
    33361 tcaagcacta cacgaaagac gcggccggcg ggccgttttt gaccctgcag ttcgacggac
    33421 atgcaaacga cgcgggtacg ttgacgcgct gcgaggcgta tctcgacagt caggggtttt
    33481 tcacgcatga gccgaggccg atcgagaggc cggcacagaa ggctctctcg cgaacacgag
    33541 aagaaagggt tgaggcatga ggagcaccgg caacccgttg gcgggacgca cactttacat
    33601 acaacagatg acttatgcaa gcacgcactt gctcgcggcg gcgttccact cagtagggat
    33661 caacgcggtc gtgacggaag agagcgacga gaggacgctg gaactcggcg gcaagttttc
    33721 gtcgggcgag gagtgcctcc cggaaaagat cacgctgggc gattacctga aggtggtctt
    33781 ctcggaaggg tttgacgcca aacgagccgc gtttctcatg ccgacggcaa atggtccgtg
    33841 tcgcttcggg cagtacagtg gtctcctcag gaaggttctt aatgatctgg ggcatcacga
    33901 ggcgctgatt ttctcaccga caagcgcgaa cggttacgat ggtttcggag cgaacggcaa
    33961 cattttcctc cgatccgcgt ggcgggcagt ggttgtgggt gacgtactca cgaaggcgtt
    34021 gctcaggacc cgaccgtacg agctgcgaaa aggagacacg gacgaggtct ttctgcgttc
    34081 gatcaggcgg atgggcgatg ttttctcgga gaaagtcgag agtgccggat cgcagttgga
    34141 gcgcctggtg agggcccttt cggtatgcca cgatgagttc tgctcgctgc cggtggatat
    34201 gagcgagccg aagcctctcg tcggagtggt gggggagatt ttctgccgcc tgaatgattt
    34261 ctccaatgac tcaatcgtgc gcaagctcga ggagtacgga tgcgagacgt ggctgtctga
    34321 cgttgccgaa tgggtgtggt atacaaatga cgaggccaag acgaacatgg atgccgacgg
    34381 cctcctgaag atccacccga aacgaatcaa actctgggcg aaaacgacgt ttcagcgcca
    34441 cgatgagcac gcgctgttga gcgcgttcgg ggattacttc accggcgtcg aggaagcaag
    34501 cgacgtgacg gaaatcctga acgccagccg accatatctg ccaaaggagg gaagcctcgg
    34561 cgaaatggtg ctttcgtcgg ggaaagccat ctacctctac cgtatgggag cggatgggat
    34621 tgtagacatc agccccttca cgtgtatgaa cggcatcgtg accgaggcga tttacaccaa
    34681 gctgagcaag gattgcgatg gcatcccgat caggacgttt tatttcgacg gaatatcgtc
    34741 ggaccttgat cgtgacgttg gcattttcgt ggaactggtc aaaacgtaca ggaggcgcaa
    34801 aaagatcgcc cgcgtgtggc cgcaccggaa agaaacgact gccgtggcgt agaacagtgc
    34861 cagtgtgcgg gggcattctg cgatcgacga atgcccctct tcccttgccg atcgcctccc
    34921 cgtggaaaat tccggccccg tcgatatatt cataattgca tagcagttgc acgtgtcaaa
    34981 taaaccgaat tggaggattc ctgtgtacaa gattgccgtt attccgggtg acgggaccgg
    35041 tcccgaagtg gtggccgagg gattgaaatg catcgccgcg gttgcgggga agttctcatt
    35101 caaatacgaa accacaacgt ttgatttcgg cggagaacga tacctccgca caggagaaac
    35161 gcttcccgac ggagcgctgg atgagctgcg ggaattcgat gcaatctatc tcggcgcaat
    35221 cggacatccg gatgtgaaac ccggcattct tgaaaaaggc atactcctgc gcacgcggtt
    35281 cgagcttgac cagtacatca acctgcgtcc ggtcaagctt tacccgaatg tggattgccc
    35341 gctgaaggac aaggggccgg ctgacataga tttcgtcgtg atccgggaaa acacggaggg
    35401 gttgtacacg gggtctggcg gcttcctgaa aaaggggatg ccggacgagg tggccattca
    35461 gacgtccatc aacacgcgaa agggcgtcga acgctgcatc cgctacgcgt ttgaatacac
    35521 acgacggcgc aacagacgga agacactcac cctttgcggg aagacgaacg tccttacgtt
    35581 cgccttcgac ctctgggagc gcacgtttca cgaagtgggt gcggaatacc cggacatcaa
    35641 acgcgaatac gcacacgtag acgccacgac gatgtggatg gtgaagaacc ccgagtggtt
    35701 cgatgtaatt gtgaccgaca acatgttcgg ggacataatc actgacctcg gcgcgatgat
    35761 tcagggcggg atgggcgtcg ccgcgggtgg gaacatcaac ccggaaggcg tgagtatgtt
    35821 cgagccgatc ggtggctcgg caccgaaata cacgggtcag ggggtaataa acccaatcgc
    35881 ggcgattggt gccgctcaga tgctgctcga cacactcgga gaaacgaagg ccgcagagtg
    35941 cctggaaaag gcgatcatcg aggtagtcgg aacgaagatg aagagcatgt ccgcgggtaa
    36001 gatggggtac tcgaccagcc aggtcgggga ccttgtcgtg gaagcgttac tgaaaagcta
    36061 acgcgtaccg tgctgtatga aacaggaaga ggagcgcagc tacaagccgc gctcctcttg
    36121 tttttggctc catgatttta ccttacgtgg taccgctgtc ctgcgatggc ggaacatcgc
    36181 cctctttcgg gccggtcacc ggttgtccgc cgatataccg cgccgccatg agcagaccta
    36241 cgcccacaag catgatcacc aacccccaac cgatctcgct ccagccgtcc tcaagcacgt
    36301 tggcgatgga cagccccacg ccgccgagcg ccatgatcca tccccagatg atcatcttct
    36361 gaacgcgagg cgcggcgggt ttgggctgag tcgccatatc aggcggaaca attccctgcg
    36421 cgatcatggc cagccgctgt tcgtgcacca gcttggactg gtgtatccgc caccgcatga
    36481 gaaagaacag ggcaaggacg cacgcggcga agaatgccac ggggacgata atctctaccc
    36541 gggaatcgga agaggggatc gtttcatggc gggaaggacg ttgaagagcg agagacgtct
    36601 cgatcagatc ctgcgtctgc tcaggcgtca gtttggccca ggcttccggc ggcaccgcgg
    36661 ccagcgggtt ctgcacatcg acctgagcgc tgtctcccct caggattttg atcatcttca
    36721 cgatgttcga atcacggccc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36841 ngcgctgtct cccctcagga ttttgatcat cttcacgatg ttcgaatcac ggcccacggt
    36901 aatcgaatcc atgtccacct tgaccgtgac ggcggatcgc gccgggatac agagcgccag
    36961 cagcgcggca aacacgaatg caattcggcc cctcataggt tcgacctccc ttggaaatgc
    37021 ccacctgcgc acgttgtagt ttcgcgcttc agatggtaag accggatgtg cgcgggaaat
    37081 gttacatcca ccaatcgttc ctcgtcggga acctgacccg cggtgccggt taccagcatg
    37141 ctccgagcgc agcttccttt tccagagccg tcgcgcgctc ttccatgccg tacgagcgat
    37201 agacggcgca ccaccgctgg tagtgctcgg gggtacgtat caccggttcg aaatccagct
    37261 ggatgtacac cttcaccgca gccgtacgga attcgtcggt agtgagatag acgtccggga
    37321 ctccccaatc ccggaacaca tggagcacgg cggcacacac ccagtaggag agccgtttcc
    37381 cctggtgttc cggaacaacc gcgaccatgt ggaccgatcc ggcgcgccag tcatcgtgcg
    37441 ttccgagcca cgcacaggcc gtggcgaccg gttcgtctgc atccgttgtc acgaaaagca
    37501 ggccttcagg gtgaaacctc ggttgtcccg tcagagtatc taccaccttc tcttccgtat
    37561 attctcctaa ggctccagca cggtttatga tgtcggccca cacgccttcg tcgccctgaa
    37621 cgtaatgcct gaggcggtat cccgaaggca atgcgacctc gggcacgtcc gcgttggaac
    37681 gacgacgcat tttcaactgg tctgccattt cacctccgta gagaactcat aatcgtgtgc
    37741 tgaatggcta ttcgaggtta agcaagggat gaaagcgttc tggcgcaatg tggctctttt
    37801 cacgcttgtg ttcctcgtag ttttcacgtc gattatcgcg ataacagtgc accttctgag
    37861 ccgtgatctc cctgacctgg cagcagttga gcagatacgg ccgaagcttt ccaccgtaat
    37921 cacatccgcc gatggccaga ttctgaagac tttcgccata cagaagcgca cgctggtcgc
    37981 tttcgatgat cttccacccg ccctgactga tgctctgttc gcgagcgagg accgcgaatt
    38041 ctggtcgcac tggggcgtga atccgatcag cttcgtccgc gcttctctca tcagcctggt
    38101 cacactgagc ccgccgagag ccacaagcac cctcacgcaa cagcttgcgc gcgatctttt
    38161 cctgacaaag gagcgcagtc tgatcaggaa gataaaagag gcgattgtcg cgacccgaat
    38221 tgagcgtacg tatacaaaga aagagatcat tcagctctac ctcaaccagg tctatttcgg
    38281 tgcgggtgcg tacggtgttc aggccgcagc gaggacctat ttcggctgcg acgcgagaga
    38341 ccttaccacc gagcagtgcg ccacgctggt gggaatgttg ccggctccga attactacaa
    38401 ccctatcagg aacccggaac gcgcactggc gcggagaaac gttgttctgg gagccatggg
    38461 agcaaccagg aaactgccga aatccgaagt tgcccgcctg caagcacttc cgctggtgac
    38521 taccccgggg ctcgaagaac tgggcatcgc tccgtatttc acggaataca ttcgcatcag
    38581 gttgtcagaa caacttcaga agtctgattc gctccgcatc cgttttgcgc aggttgcagg
    38641 acttcccgac accgcctcgg cagaccagct catctacgaa ggtggcttcg tgatcgaaac
    38701 caccctggac agccgattgc agcaaatagc ggagcgcgtt ctgtttgcgc ggatcgactc
    38761 gatgcaggcg tattacgatc gtgccgtgcg cgcccagaac aacatcaaca gcccgtgggt
    38821 agaccgggaa cgaagccttg cgacgggctc catcgtagca aagcgtgtgc aggccgcgct
    38881 tctcgcgatg gacgtgcgca cgggcgcaat tcttgccatg gtcggtggcc gtgattttga
    38941 cgcgacgaag ttcaaccggg cggttcaggc attgcgacaa ccgggaagca gtttcaaacc
    39001 gtttgtctac acggcggcaa tcgacaacgg tttcagtccc gtcacggaaa ttcccaacca
    39061 accggttact ctgtcggatc ctcagcgggg cgaatggcgt cctgagaact acgatgggag
    39121 tatcggcggc ccgatgacgc tcagggaagc gcttgccaag tcggtcaatc tggtcgcgat
    39181 tcgtctcctt ctccagacaa ctccacggct cgtgattcag tatgcccaga acatgggcat
    39241 aaaaagcaat cttccgccgt acccatcgct ggcgctgggc gtgggtgaag tgcggctggt
    39301 tgacctcact gcggcgtatt ccgtgttccc gcaccagggc atctatgtgg agcctttcgc
    39361 catttcgcgc attatttcca gggacgggat tcagcttgtg gagcggcaga tttccggtcg
    39421 acagcacgaa gcgttgaatc cgggaaccgc gttcatcatg acaaaactgc tcgagaatgt
    39481 ggttgtccga ggtacgggtg ccggtgcacg ctctcaattc gggctgcttc ggcctgccgg
    39541 tggcaagacc ggcaccacaa acgattatgg tgacgcgtgg ttcgtcggct tcactcctca
    39601 gatctgctgt ggcgtgtggg tagggttcga tcaacgagtg aacatgggac ggggaaacac
    39661 gggtgccgga gcagcactac cgatctgggc gcagttcatg aaggaggccc acgacgtgct
    39721 caatctgccg gcacaggact ttccaaaacc tccagaaatt gaggcagtag agatctgtga
    39781 cgagagccat ctcgccgcaa ctccgaattg cccgagtacc tacacggaat acttccgttc
    39841 cgcggaaatt ccggagccgt gtacaattca tcgctggggc agaagctcac cggcttcacg
    39901 ccgtgaagca ttgcggcgta actattgagg cttgtccgga gggcgttgaa agccggattt
    39961 ggagcacaca cctttgttct cgagctccac gcacctgccg actattttga cggagtgatc
    40021 cacgagttcg tatcccatgg tgacggcaat ccgtcggagg tgcccgagaa gaaccggatc
    40081 ggcaacctcg aacacgcgcc cgcactgctg gcattgcaga tgctcgtggg gggtttccag
    40141 cccgtagacg agttcgtagt actccgaggc agatccgctg ggggcgatgg aggagcgcag
    40201 tattccgcaa ttagcaagca aggcgacggt gcggtacacg gtggctttgg atatcctgtg
    40261 ccctgcatcc cgcaggcgaa catgcaggtc atccacttcg aagtgcgtgt gggcggagaa
    40321 gatgtgctgg agaatatgac gccgctcggg cgtcattctc attccccgcg agcgcaaata
    40381 ggcaacgaac tcggtgacaa gcgcctgcgt attcattggt cgtccatccg tctcaggttc
    40441 ttcgtcaaat tgaagatacc gcacatcggc gaccggtcaa cagcgtccgg cggagcgttt
    40501 ttcctcgtgc gagattcggc gcggcgcaga gacgcacccc ctcgccccct tgccggaaaa
    40561 aaagtactaa ttgatggggg gcatcgcacg ccggctgcgc tctttgcgcc gctatgaaac
    40621 tggcacgttt ccgggaaggg cgagagaatg gaatgcactc gtgccgccgt ggtggattac
    40681 cgggcgttgt gtgagcctcc ggatttttcc ggcgacgccg tggcagaaca gacgcgattc
    40741 cctattgcaa ttgaagcgag agtgttgcag catcttctcc cacgtgaagg cgtgtcacct
    40801 gcctgggcct ctgattttgg gcggattttg tcagattcag aggcaattcc ggtaaccacc
    40861 gttgacgccg ttcttgtgcc tgatgacacg cttaccgaag atctgcgcgt gagtctcgag
    40921 gaacttgacg gcattgcgca gcggtggggc actgttgtcg cgcacctgat aaccgcggtg
    40981 cccaggaaga cactctcgtc gatcgtgcat caatatttgg cagccgaaga cgaacaagtc
    41041 ctgcgctttg gccagcaagt ggaaaaagtg cttgcccttc cggaggacgc tggcaatctc
    41101 gaacgctgtc ttgccgtgat tgatcacctg ggcgcgtggg tggatcgtcc ggcgatgtgt
    41161 gatatcgccg atgtggccta tcgtcttcgc atgcggattg acgaagatcg cgctcttttg
    41221 cgccaggaaa ccgaacggca gatcgcagag gcacccgcga aggcgctcga cgagttccgg
    41281 gctcagacgg ccgtgggctt ttgcctgccg gaacagcgca tggcgttccg gggagacacg
    41341 cttttcgttt tcgacgggga atacccggcg gcggagatgc acgtgttgat ctcggaatac
    41401 atggctgacc tggagaaacg gttcaccgcg gcagcgattg accgggacct tctcaggcag
    41461 gcggcagaag ccgagcaacc cgcctccatt ccggatcgtg tgcggtccat cgtgtggcgc
    41521 cgggataacg gcaaatgcgc gatgtgcgga gggaccaccg atctcgaatt cgaccacatc
    41581 atcccgaagt cccagggcgg ggcatcaacg gtcgcaaacg ttcaggttct gtgcgtccga
    41641 tgcaatgaga ggaagggcgc acaggtggcc tggcttcctt ttcctcgcgc gcaccggacc
    41701 tccgaggaca cactggatct tttcagaacg cctttgcccc cacgactaga acgcgattcc
    41761 acatgctgat tccctccagg gctgtccatc gggcaccaca cgagcacaaa ggaccacact
    41821 atgccacaat actccgtagc catcataggc acaggaaaag cacaccgatc tgatggggcc
    41881 accgggtatg gaaccgcaca ctggcacgcc cagggttacc ttgcggcacc cggatgcacg
    41941 ctgattgcgt gcgcagacat cgttccggaa aatgcacgcg cattcgcgga gatgtacaac
    42001 atcccgcatg tgtacgtgga ctaccgtgag atgctcagca aagaacaacc ggacatagtg
    42061 agcgtatgca catggcccca gcttcacgcc cagatggtag ttgattgcgc caacgccggc
    42121 gcgaaagcgg ttcactgtga gaagcctatg gctccaacct tcggcgaagc gcgcttgatg
    42181 aaggatacct gcgagaaaac gggaacacag cttacgttca atcatcaacg ccgtttcgag
    42241 gagcggttca tcttcgcgcg cgatatgatc aaggcgggtg ccgtcggaca attgcgacgg
    42301 ctcgaaggat cctggggcaa catgctggac gtcggcacac actggatcga cctgctgaac
    42361 ttctacaatg acgatactcc ggcggactgg gttattggcc aggtataccg cgccggagac
    42421 cagaaagcgt tcggtgtcga tgtggaaagc caggcgctgt gctatttcca ttgtgcgaat
    42481 gatgtgtacg ggctcctgct gatggggcag gggcattcca ttggcgcaga gcaccgcatt
    42541 ctgggaagtg aaggcatcat cgaagtggat tggccgcgtg tgagaattcg aacaaaaggc
    42601 gatgcatcgt ggcaggtttt cgacaaaggc gctaccgagg ggtcggtaac acggggcatt
    42661 caggatgtcg tggcgagtct gagctcgggg aaggaatccg cgctgagcgc gaagaaggcc
    42721 tttgcggcga ctgaagtgat attcgcgacg tacgagtcca gcaggcggcg gggccgggtg
    42781 gaattgccgc tcacgattga cgacaacccg ctgcattcga tgatcgccaa tggccagata
    42841 gggtgacggc ggttggctgt gatgagacaa gaatggttac ccagttccac cagtgacgga
    42901 gacgagacac aatgacagaa cttcgagcag tgattattgg ctgcggcaag tcaacgcgaa
    42961 ctgccggcgc caaggaacac ggaatcagcc attgccacgt gcaggggtac caggcgacgg
    43021 aaggctgcaa agttaccgca tgcgcggata tcgtggagga atacgcggcc agctatgccg
    43081 ctctctacgg gttacccaag tactatctcg actaccggga gatgattgcc gcggagaaac
    43141 cggatatcgt gagcgtgtgc acctggccgc acctgcacgc ggagatggtg atccactgcg
    43201 caaaggccgg cgtgcgggcg attcattgcg aaaagccgat ggcaccgacc ttcggtgaag
    43261 cacgcagaat gaaggaaaca tgcgacgaac tgggcgtgca gctcacgttc aaccaccagc
    43321 gacgcttcga accgcagtat cgcacggtca gagaacttgc caaccgggga gttgtgggca
    43381 aagtgctgcg catagaggcc gcctgtgggg acatgttcga ctggggcacc cattggtttg
    43441 acatgatcaa cttctacaac aacgacgttc cggcagagtg gattatcgga caggttgaca
//
LOCUS       DAOSOO010000003        42336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_961_length_42336_cov_5.775005, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000003 DAOSOO010000000
VERSION     DAOSOO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 42336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42336
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_42336_cov_5.775005"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..920
                     /locus_tag="PLT86_00360"
     CDS             204..920
                     /locus_tag="PLT86_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35100.1"
                     /translation="MNEDTVSGDFLKPSKRTPVRVTVRNPEREKAGQLTPKETEIPRL
                     AEEKAPQTRVVYSTRGYEYEAPFNYPNVNCELGRFGTGTGAHPDGMELDIPPFGAITI
                     EEAEPIIGVTVIGSPCIPPGTILVFGEPLEWKYGRSGVKFKQTNIWGEHLYRWGANPE
                     FEEGNRHAGLASVHFEIPDCRKVTVMGYGQLDVHVSPPEWTPGKGRQGLEQYEMPGED
                     WWNDAHYNVRTIRITVRVPA"
     gene            1053..2423
                     /gene="mtaB"
                     /locus_tag="PLT86_00365"
     CDS             1053..2423
                     /gene="mtaB"
                     /locus_tag="PLT86_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT35101.1"
                     /translation="MRVNFLTVGCKLNNCETEVLERHFSRAGWDVVSREEPSDVTVVN
                     TCTVTARSDADCRKLIRRAKSMNPAGRVVVTGCLAERTPDALAGMPEVAAVIGNRAKK
                     NLLSIVTAELQKASGPLIASQDQSGPGFLPLSPRVGSLSATRTRATVQVQEGCDEHCT
                     YCIVPRVRGRSRSRPIEDIVAETETLVAGGSVEIALTGVNTGAWGLDLGAEGGIAAVV
                     DAILRVHTPSGQRVERLRLNSLEPQAITPDLVRKCAMEPRVCRHFHVPLQSGSDAILR
                     KMNRRYTAAEYAQKIELIAEHIPDVAIGADVMVGFPGETDKQFGETFELIRRLPLTYL
                     HVFPFSPREGTPALRLGPAISSERKQERSRALRELGNRKRLQFHLRHVGNVVTVLLEG
                     KPVTATTVLSGLTDNYIRVAVRAPSDRINTLVPVYVEDASREGVRGVLVDECSTSFSR
                     TRGSQE"
     gene            2420..3808
                     /gene="miaB"
                     /locus_tag="PLT86_00370"
     CDS             2420..3808
                     /gene="miaB"
                     /locus_tag="PLT86_00370"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT35102.1"
                     /translation="MRVRQLDVLNCDIGANESSASTSGHMRVFLETYGCQMNVADSEL
                     ITGILSRAGFQMVSGPESADVILLNTCAVRENAEERVFGRLGELTQYKNSRPHVLMGV
                     VGCMAEHLREEILGRAKAVGLVVGPDSYRRLPHLIESALAGPVVDITLDKSEQYLGLE
                     PDRGGGVTAWIPIMRGCNRFCTFCVVPYTRGREKNLPPEEIVRQVEHAVNLGHSEVCL
                     LGQTVNSYRHGDTDFADLVRMVGAVDGIRRLRFMSPHPVGFTASLVDAIAEVPAVCPH
                     VHLPLQSGSDAVLTRMRRDYSAQEFRAVLSDLRRRIPGISVSTDIVVGFCGETEDDHR
                     RTVEFVEESRFDFAFTFMYSPRAGTFAARFLKDDVPAEVKRRRISEVIALQERISADI
                     HAKLVGSVREVLFEGSAKKGDAQSFGRTADFKGVVVEGSFAPNSFRSVRITGANAHTL
                     TGEVLPDTGARP"
     gene            3808..5016
                     /locus_tag="PLT86_00375"
     CDS             3808..5016
                     /locus_tag="PLT86_00375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT35103.1"
                     /translation="MIPRRTRVTIELAAAALFLCANRVPAATGTVVVVANGGNRVISS
                     SVEHGVTFVSVPDLARALELQTEWDARTRTLVVLSGETEAEFIAGNTLVVVGDSTFHL
                     RHGTVLRAGELLAPADALPGLFGLLSPVALLWDDRERVFRTSADFGSGAGDGVTDDTH
                     PNGRNRWEIDRVVIDPGHGGRDPGTLSVEGVPEKEITLAIALRLAQLLRDELGVDVVM
                     TRTDDQSVSLRERGLAGVRAGGKLFISIHCNAIDKAHVSGVQTYFLSDAETDEARAVA
                     RAENAALMYEEHADTLSEGDDREMEEILAGLVSDRFLKESQELAALVQRELVRGLRTR
                     DMGVHQAGFYVMKGTLGAMPSILIETGYLTNQDEAARLRDARYQERIADAILRAVREF
                     KMRYETELGR"
     gene            5026..5826
                     /gene="murI"
                     /locus_tag="PLT86_00380"
     CDS             5026..5826
                     /gene="murI"
                     /locus_tag="PLT86_00380"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT35104.1"
                     /translation="MDRRPIGVFDSGVGGLTVVNEIQKQLPKERVVYFGDTARFPYGS
                     KSVETVTEFARQDSQFLLGFDVKLIVVACNTATACALDHLREHLSVPVVGVIEPGAVA
                     AVRASRRKFVGVIGTQGTIASGAYQRALTRIDPSISVFAQACPLFVSLAEEGWTDRAA
                     SLLIAHEYLDPLREAMIDVLVLGCTHYPLLMPVIRRVMGPETILTDSAAEVTREVGRV
                     LQENELESDGSAVSGHRFFVSDAPQKFREVGERFLGRALPTVELATAG"
     gene            5923..>6461
                     /locus_tag="PLT86_00385"
     CDS             5923..>6461
                     /locus_tag="PLT86_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT35105.1"
                     /translation="MERKDFLNPAGIYRPAPFWSWNDDLRNEELEAQALDMKKRGWGG
                     YFMHSRIGLVTPYLSDEWMDRTRHTVELSAREGLCAYLYDEDKWPSGYAGGLVPRSNP
                     AVRNTALQCHPVPPPEDTNTVLAVFARESGRWVRIDPASVRSGTELAYISQWVEPMGN
                     PWFNGTTYVDLMNPEAFEE"
     assembly_gap    6462..6561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6562..9005
                     /locus_tag="PLT86_00390"
     CDS             <6562..9005
                     /locus_tag="PLT86_00390"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT35106.1"
                     /translation="FNGTTYVDLMNPEAFEEFARSTLDPYAALLGAHCGTTIPGCFTD
                     EPAYIFWHARGIHPSNTVPWSPGFRERFTGRWGYDILDRIISVFKREGDFARVRYHFW
                     RTATEMFLDNFSEPYGRWCREHNLELTGHYMCEDTLVDQIQWVGAAMPHYEHMGWPGM
                     DHLGRNIDNIMTAKQVTSVAHQIGKGRALSELYGCSGQNLTFEGRKWIADWHFVHGIN
                     LLNPHLALYSMRGERKRDYPPTISFQQPWWRFNNLIADYEARLSYALTRGKRISQVLV
                     IHPIESAWALYEPGDDRRARQLSEWFDQVSRWLLEEHYDYDYADESLLAKYGRLEHDR
                     LFVGDAEYELVVIPPCFTLRRTTVDFLQKWMAAEGAVISMKPVARRIDAETDAWDFVR
                     DTIVVENDRTAFTSVLGELLEPNVRILSPEGYPVAPVWVHQRADGEKDLFFFANTDRT
                     RGYACEILLQGDGAVEEWDPRTGDVHEVAVEVLDDEVVVSRFIEPCGSLLLVMDRSKD
                     AREIDEDEEEFFEEGDEIDLDGSWLSEPRDPNAITLDTVRWRLGDGELQGPDPLWKVL
                     HPVRSHSGTFSLEFCVSVEVVPESDVFLVLETPEAFSVSVNGTELPQTDCGFWVDTSF
                     RKRSLNGLLRMGENTIVLSGTADPKIELESLYVIGDFGVRTEDNRAFVITSRKSVVLA
                     QNLVEEGFPFFAGTISLTQSFEMNLSASGQAKLVFDDPQFVVADVWVNGNNAGQVVWS
                     PYEVEIGRFLVDGTNTIRVDLVNSLRNLLGPHHHAFGELLGVGPDSFSDAENWTDVYQ
                     FVPFGFGGARVIVQS"
     gene            complement(9073..9843)
                     /locus_tag="PLT86_00395"
     CDS             complement(9073..9843)
                     /locus_tag="PLT86_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35107.1"
                     /translation="MLKKFFWNLYDHLGTCVVLNLLWFLASLPWFLAIWSAGRLLVSS
                     FGAVGFVTALAAGFAAIWLNPATLTAMFCATRWVRYQRVERADVWRFFRSQLVPGIAL
                     PIAAALVFVILGANAAFYLRASWMPRFVGLLLAGVMLWAQIAFLLVAFWFGLNRAAGD
                     DGARFREVVRDSFLLTFRYPARSAGLLISTAILGAAMALTQVGLLLIAMSVAAVFAAT
                     GRRELLRAMRRGEGEGNPDTPEEPRTFRDLFAPWEANR"
     gene            9970..10743
                     /locus_tag="PLT86_00400"
     CDS             9970..10743
                     /locus_tag="PLT86_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT35108.1"
                     /translation="MFVENVVYRPDSERGVLDIMLPEGAGPFPAVLCIHGGAWSGGDK
                     SMMHPFGEEFLKLGIASLFPNYRLTGTHPHPAQEQDIFAALEWAAANAERFRLDVTRF
                     GITGASAGGHLTALVGLKATKKRDIGYTVRCMAPVCPPTDFFAFSRDNPSLRSVLEAL
                     VGGELEKNEAVIRDVSPIFHVHEKAPPCLVTHGAIDSVVPPAQATSFVDALKSVGVEA
                     EVILVPGVDHAAFNPGTDPPEPLGGIKAFRAFYRKHLLS"
     gene            10797..13253
                     /locus_tag="PLT86_00405"
     CDS             10797..13253
                     /locus_tag="PLT86_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35109.1"
                     /translation="MNPDYRFHGRSMTFITAPDATQAERHVARVASEAARRRGVTGHC
                     HESGDVPSTDLAVFLGVGETHPHVVEELSRRDRFAPSSPLTRGAQSSRIVSCVNQSAD
                     AVAIWGADIEGLWVAVGRFLRGLRWKDGAFSSAGWDDEFRPAFELRGEVMSSHFTANT
                     FFQWSPRQWFEYMDEMALWGMNLFITIPIHMADWDGISPWSDPPRWVSEERRKEWDTH
                     WETHCAVSQRVHEMGLRYGLWIPANDVPPMMAEADWNRGGGKYVCPSVPEARRAIQYT
                     RSRVIQGLPHVDYWFVPSADNGGCPCDDCQPWVDTYLSLAEEQFRELAKWHPGAKVIL
                     SNQALTHEENKKLCARLAEPGTEWIEGVAYAPGSNETYIQARETEEYDWIDWYQFDAE
                     HRSLKELSRTMPPSKKLFLYPDITHLTRSQYPVWDVDPLVAWTYHRDTVFFRPRGYAE
                     VFRRSAPHADGTFPYSEGIYDDLNQVVWLTLSCEPGRTHDDILSEYWRWHAGEDAVFP
                     AVCATHQLEDSWRGDITANAQVETAVLETEKAVRLARGTGWRLDLLRMRALLDEWLKN
                     RLRAAKLLESGIIAMLRDDATESGVLRAIESLDSAIRSLPEASDEVLRLGESLLRAGP
                     RMPVGRVRHVTGNLAWMKATLKKALVDPSVRSALVADVIDYERGVYYVDCGNPVRDTF
                     CVHGRRYTCLDERSYQTPFSEAPEIRPSQNSLVMTCGEDDGIVYRFPDLHPDRKYVVR
                     LTYFLPTLFTYRETRSQKLEANGVEVHGPLVLPRKRPETFEFALPRASYSAGETTLRF
                     RKAEGGVTTVVSEIWIRECD"
     gene            13284..14648
                     /locus_tag="PLT86_00410"
     CDS             13284..14648
                     /locus_tag="PLT86_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35110.1"
                     /translation="MPTTVGRIEETVLEKYGDFHNGAGPMWCFGSHTIVRSGDTVYAS
                     MVDVEPEARPLCNAKWQLWQRQDGGSWGKIAEQQKAGEREPCPIALGPGGTLLLSVST
                     AVSPSHDNHDGTTGFHCLPHVLSFRIAGGILPEPKVIIPQWNAEYPFNEHSYRGFSSD
                     ASGNVFLTHQASDGSEYGQAWCYRTSEGDWVAAGFLRFPMRGCYPAAAVNGKAVAVLA
                     ISDEIEPNEEWRSFKKEKTGRDWDYDFRQLFFTWTPDVTAVPFSVPLTIASVDETCGV
                     ITHTDLLVTPDGDVHVLYIERNVYHDFVRERFFPDLPLSVSLKYVRLRRGCVVERRIL
                     CKSCEKFDNTRAETAVKMGAPVPTWAVFHATGAGRVFLVWNQTDNVASQNGMFLRQII
                     PQWDTTAISLPVRTPLTQFFAASTRAGCLPDNRLDLYGTAPGEQVIRTIHCVVETDQA
                     EVTR"
     gene            14645..15886
                     /locus_tag="PLT86_00415"
     CDS             14645..15886
                     /locus_tag="PLT86_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35111.1"
                     /translation="MTTPRERVARAVNFQPTDRVPIDLGGMKATGIAVKAYNAVKKQI
                     GLSSPTRIWDPKFMIAHVEEEIRQRFHLDVVPLDVSSVSDEMRPQREWVPMALYEGAE
                     GLMPPGTMIGEDDRGRWVLMGADGRPTTLRMPHEGYYFDDISFDRPGMEIRPSEFQPE
                     ATFSDEQLRAVEARADFLFSQTDYAVLGWGGGVCFLGLSLITDRSSNVTMNCPTQWMT
                     MLITEKELCHEMMGRSVDAAISRLSLLHEAVGNRCVAWGIAADDAGTQRGEFIRPDLW
                     AEMIKPHYARLCSWVHSHTEWKTFLHSCGSVYHLIPHMIEAGVDILNPVQTSAANMEP
                     GRLTSEFGGKLVFWGGGCDTQHVLGNGSPEEVRTHVRERLELFKTGGGYVFTQVHNIQ
                     PNVPPENIVAMLDAAYEYGVF"
     gene            complement(15890..17128)
                     /locus_tag="PLT86_00420"
     CDS             complement(15890..17128)
                     /locus_tag="PLT86_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOT35112.1"
                     /translation="MLLFCGAEVSRADKLLIPMDRTQTDHLKAYGVVFWSLQRGLSAE
                     WLLNYRGGSFLLDYSDVIRAESQLRGVLSERIADADVAEIYGTIERENMEVIRLDKAP
                     RIAVYTPPNKQPWDDAVTLALTYAEIEYTTLWDREVLAGELRNYDWLHLHHEDFTGQF
                     GRFYISYQTAPWFLQQKAELERTARELGFAKVSELKKAVARAIREYVANGGFLFAMCS
                     ATDTIDIALAAEGVDIVPAICDGDGVTPDYNNRLDFSKTMAFENFRLEENPWVYEFSD
                     IDTYDDRAANPRGPEADFFTLFDFSAKLSPVEAMLTQNHVDHVRGFLGQTVGFHRRFI
                     KPSVIVLGESEGEDEVKYVHGQVGTGTFTFLGGHDPEDYQHLVGDPPTDLNFHRNSPG
                     YRLILNNVLFPAARKKEHKT"
     gene            complement(17164..19011)
                     /locus_tag="PLT86_00425"
     CDS             complement(17164..19011)
                     /locus_tag="PLT86_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35113.1"
                     /translation="MDVTFLLRAVSLRTSVVTRIWSECALGANAILLILLHTAAPAAV
                     TPPREIIGWFVQAQALEHANRPLDAAAYYRRVWQAAPTRSDAVLRGTRLLTENGRFDE
                     AVAWLGEATANTPEDAVLWVEFGDVFDRTGYTVQADSVWRIGSRTSSDPIAFHIGVAD
                     RLVSRGQLRRAQTWLRACNAQGDGQRVVNRRLLDIALALGDAHATAEAASGFVAGDVD
                     RVREAREIIQGASLTPAMMQTVTRAAIALSDSTPRDAARALFAAEMAVLGAQQEDAVR
                     LFLRSAKETRNPGATLATMIDELEARGQTPVATFLMERLASDYPESPSAQRYAYTAAG
                     ILDEMGFQDRARNLLSWIISVPFSPYHDDARIALGRIFLALDSVEQAEQQFDWAATRG
                     RTPAHRRQGILGRGECALRAGHLEEAQSFWQMLADSAMKEPESLKAHLRLAQAAMFRG
                     DAPGLRARCELMVGNAAPGDETNDCLRLAEILEQAADDSVSWVRYGHLEFLFATGHRD
                     SVLLLAPRLFGTPLEGWARLLVAEVQIREGHYSLAATTLEAAASRLDSSSAGEEALWR
                     YAELQRTRLQDSGEETRALETLLMTYPGSVYGNQARRRLRELRAQPGAL"
     gene            complement(19092..20138)
                     /locus_tag="PLT86_00430"
     CDS             complement(19092..20138)
                     /locus_tag="PLT86_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT35114.1"
                     /translation="MSLLTQSTFRRHVLEVCVALGAALNLSCSEQRTKLVVYSPHGRE
                     MLSEYERMYEEANPHVDVTWYPMGAEVGIERLRGEKNNPQADLWWGGPSALFEQGARE
                     GLLAPYHPSWREAAPPGSFSDEGLWYSTFQTVKVIGFNSDRLTEQTAPQDWDDLLEKR
                     WEGKVIIRSPMESGTMKSVFAAMVYRFFAEDGTPDRGYDWLRRLDANTKAYAASPATL
                     MLMIGRQEGLVTLWDLTDILLQKREQNVPMGFVIPRSGALVLNEGIAIVNGAPHRAEA
                     ERFYEFVTTKKALLHQAERFYRIPARRDISDQEKPGWLRSIQITELPVDWNVINAHRE
                     AWMQYWDQHIKGHH"
     gene            20336..21022
                     /locus_tag="PLT86_00435"
     CDS             20336..21022
                     /locus_tag="PLT86_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOT35115.1"
                     /translation="MITVWDWVILALFAALVAQDTTAGPQLLFSEPLVAGTVAGLVLG
                     DLRLGLLLGTAIQLIWCGAVPAGTAVFLDINVGTVCAVAVAHSAGSAPIAAAASMLWL
                     IPVGLLGCALTEVGMRFCGKLVASIQPECVSAGALVLRHGAGWLFAGARGSVTFLAGA
                     GAGVAVAPVLVRFLGAAVDPGVLWAGLFGAGAGSAIAATWRFSKGKAAFLGAAGVFGA
                     WAGGLLPVSW"
     gene            21035..21841
                     /locus_tag="PLT86_00440"
     CDS             21035..21841
                     /locus_tag="PLT86_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOT35116.1"
                     /translation="MTSLTRVSSSETAPVVPLWVRMGVVLRAFWIQASLNYQSMQGLG
                     RLVCLLPVMRWLSLPGKEFASFVRRHSGLFNSNPFVATLGLGALARLEFDRNANPESV
                     PDGMIERFSERVSTPLGSVGDELFWVTLRPHAVLVGSVGALLTGAWGACLLLVVFGVW
                     QGYYRWNSFTWGWAAGARIAAVLQSERFRRPAKYAGWIASGCAGAAVALLLVNSPAGA
                     LTAKAQVVAFGGAFAGACAALSQRKPATWALLAAVGWAIVVSFGAKMLRG"
     gene            21878..22159
                     /locus_tag="PLT86_00445"
     CDS             21878..22159
                     /locus_tag="PLT86_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT35117.1"
                     /translation="MATHQVVEADVLVKDREGIHLRNAAALVRAAAQFPKADLMISKD
                     GHQVNGKSIMGVLTLVAEHGAQLHLKARGEEADKLIATLVAMFENGTDQ"
     gene            22220..24028
                     /gene="ptsP"
                     /locus_tag="PLT86_00450"
     CDS             22220..24028
                     /gene="ptsP"
                     /locus_tag="PLT86_00450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT35118.1"
                     /translation="MDPRLPPIEPATLTPHGHCRIVLTGIAGSPGVAYGPVYLHAGAD
                     VWVDNRPIPSEAVESEIARFIAALAAVVEEKRELKRRAEREIGTAQGQIFESHIMLLE
                     DADAIAQTVECVRASKKNVAYCYFVTLQRVIDTIVGTGSYEYLRDRVVDIEDVRAAVL
                     MKLRGAEPQGLDKLEDVSVVVAHSLTPSDTARMEASKVVGFVTDRGGPTSHASILARS
                     MGIPAVVGCQGASVTISPGEMAVVNGFTGEVYVEPDEVLREQIAQWKQQLEQRARRLL
                     EISRLPSQTRDGRRIRLELNIELDSEIQRTAELASDGVGLFRTEFLFLSSDEWPTEEE
                     QYAVYRHLAEAFGGRPVTIRTMDIGGDKLCRKLHVTPEMNPFLGWRAIRISLAQPELF
                     GVQLRAILRASAHGNVQMMFPMISSVEELDQALMILGQAKEALRRNGTPFNDSMPVGV
                     MIETPAAVMIGDALAKKAAFFSIGTNDLIQYALAVDRDNQRVAKLFDMFHPAVLRLIH
                     AAVKAGHLARIPVEVCGEMAHVPLGALLLVGLGVDALSMAPGSIPEIRRIIRGITFAE
                     VKEVARAALDSVSGEEVRRNLERAAADFGLDIGVES"
     gene            24050..25207
                     /gene="metK"
                     /locus_tag="PLT86_00455"
     CDS             24050..25207
                     /gene="metK"
                     /locus_tag="PLT86_00455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT35119.1"
                     /translation="MMPRNMLFSSESVAEGHPDKVADQISDAILDAMLVQDSKSRVAC
                     ETLVTTGLALVAGEITTKAYVEVPDLVRSVIRDIGYTEMGYGFDADGCAVLTAIHQQS
                     PDIAQGVDEGEGKEQGAGDQGMMFGYATDETAELMPMPIMLAHRLVLRLAEVRHSGML
                     PYLRPDGKAQVSVRYENDRPVKVETVIVSTQHNEHVSQQQIREDVISHVVKPVVGEEL
                     LGANTVYHINPTGRFVVGGPHGDTGLTGRKIIVDTYGGFSRHGGGAFSGKDPSKVDRS
                     AAYAARWAAKNVVAAGLAKRCEIQVAYAIGVAKPVSVLVETFGTAQIDESRIEKAVRE
                     VFPLTPKWIISELDLLRPLYRKTASGGHFGRNEFPWEQTNRIAELKTAAGV"
     gene            25419..26966
                     /gene="xylB"
                     /locus_tag="PLT86_00460"
     CDS             25419..26966
                     /gene="xylB"
                     /locus_tag="PLT86_00460"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT35120.1"
                     /translation="MRSLVIGVDLGTSGVRAVAVDRSGTVVGSAARGYQVHAPYPGYA
                     EQDPEEWWRSTCEALRECVAVAGQSTIEGIGFSGQMHGTVLIDPKGSVVRPAIIWPDQ
                     RSGESCRRFVARVGADWLAARTQNSLATGFQLASLLWLSENDPSSLARAFRVLLPKDF
                     IRGRMSGVWATEPSDACSTLAFDSGRCAWADDVIAAAGIRRDLFPECRSSCDSAGCLR
                     EAAAAETGLPAGIRLFMGGGDTPSTALGSGVLSKGQCMVTIGSGGQLVAPLDEPRYDT
                     ALRTNTFCHVVPRTWYAMGAILSAGLSLKWLREKILGPDAPDFAHLAASAEQVPPGAE
                     GLIFLPYLTGERTPHMNPDACGVFFGLTPRHTAAHMVRAVMEGVAMALADGLDIIRSL
                     QVTPESVFAAGGGAKSQLWRQILADVFDCPIAVSEERERAAIGAAMVAATGVGWFENL
                     EKASDAWRAPVKDVATPQAGQVEHYRELRALFRQVYRSLEECMDARAESLRNRQGNPD
                     PFYGSSE"
     gene            26963..>28099
                     /locus_tag="PLT86_00465"
     CDS             26963..>28099
                     /locus_tag="PLT86_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HOT35121.1"
                     /translation="MKTPLKVTISPSHPLLILMSPGGPSAEMAAEGFRDEEAVMVRCW
                     ELLDDEIRPCTTVHFGATRGDNFAHADRLLRAAQTAGIPVTLQTQTDNANIQDAMPPE
                     RVRRFLDTYPCIVGLQIDEASQRTFVNHGGGPEYTMGRNARYARDIIRLAAEYGCFMS
                     WQLMRDNWAAIGCSADNEALYDAICEYSEYVIPMHEMNCEFSKFINHLSCMGLWLAGA
                     TQQWGIEAQSWYWYDCGYNKPGTCEPGTLEMPGELYAIMFLLGVSAGASVFSIEPPTD
                     NWPGPGHWRFTEWIAPVFQRLLREHLIPSVEEILASTPLAYHLPRCERPVDYHKVLAD
                     LDFDHGEGRLIRVTYGVFDRARDAEFIPNNPRYGWIPVLPAKTPE"
     assembly_gap    28100..28199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28200..28664
                     /locus_tag="PLT86_00470"
     CDS             <28200..28664
                     /locus_tag="PLT86_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35122.1"
                     /translation="EAMVGWKSAVESEEIPRIMAFYAEDYREPDGRTRESVEVAFRNL
                     FRRYVMDRFEPFLQEWGAVPGWQFPALRLLIREWGEASSQTVEVSAVAHLWAGGGPEL
                     EPSDMIIIPFGRPSLTTFVWTNAGRGWKLAKTAPAFLQVEDTAVFRFRYQGW"
     gene            28759..31026
                     /locus_tag="PLT86_00475"
     CDS             28759..31026
                     /locus_tag="PLT86_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HOT35123.1"
                     /translation="MKTPLKRTICPSHPLLILMAPAGQGGHLATRGYTGEARFMVECW
                     HRLPDDIKPYVSIQIEGICDDHFRRNEVLLPIAQAEGVPITVQVQTNNSDLNDTVPMD
                     TVRRYVDTYSCIDGLQIAEASQRTFVAHGGGPEYSMGRNARYARDIIRLCGEYGLFMS
                     WQLMSENFAAIGCSADNEALFDTVCEYGEYVIPMHEMNCEFAKYIDHLSAFGLWFSGA
                     TANWGVEAQSWYWSDAGLSTPGSFEPGSLEMPGEMYAIMFMLGAVGGATAYSVEPSWD
                     IWPGPGEWRFRDWVVPTFRRLVSERLIPDRHNVAKVTPVAYHLPRCERPLEFHAVSDD
                     LDFDHGAGRLIRGTFGVYDRARDPELIPNDPRFGWFPVLPVKTDRSVLTQFKRVLRPG
                     DVNSPEHARELMGKYYPPVDRGTAWSQIVGDLVFAANTHENWFVPEEVRLQVPRRPAN
                     VRIESTGTAQLRLRWEKAEGDNAYRVWRIRDGVETCLTPNSITETEFLLPPVEQADQF
                     AVSAITSATEEFAQTLHLHQFLVLNRAESRRSEWVNAAGDRMERFRFAESVPPVSEHV
                     LEAERRCAGCLPVQDLTSPPVTPDDPFASVKLAIMDHMAQWKSFVEAEDLDGIMSMYA
                     ADYSEPDGRGKDWVEAAFRLVLRRYMESQFARLVKEWGALPGWRNPAFRLLVREWRSV
                     SNDAAEVLVVAHMWAGGGPEMEPSDMFNHPFGRPHTTTMRWKLISGEWKITSTDPAFL
                     RIEDTAIFRFRYQGW"
     gene            complement(31088..31867)
                     /locus_tag="PLT86_00480"
     CDS             complement(31088..31867)
                     /locus_tag="PLT86_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35124.1"
                     /translation="MGNLKVGVMIESFRLGVRNGIKKAAEIGADGFQIYVTGGEMHPD
                     NLSKTGRQEFLKFVADQGLVISALCGDFGKPYTKEEGLDELITDTKKVIDLAVDLKTP
                     IITTHIGVVDADPKARSRQIMADALNNVGAYADNRGIMFATETGPEPGPVLADFIKSL
                     KTQAVKANFDPANLAMMGFDVLEGVRALGPYIVHTHAKDGIREDGRGKEVPLGEGAVP
                     WEEYIDLMTAQGYRGFYTIEREVGDDPAADVKKALDFLRKW"
     gene            complement(31923..33299)
                     /locus_tag="PLT86_00485"
     CDS             complement(31923..33299)
                     /locus_tag="PLT86_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT35125.1"
                     /translation="MATNVIVVFGDEMRGQAMGCAGDPNVSTPCLDSMASRGCRFPRA
                     YSNTPVCTPARGTMLTGLWPTAHGAVVNDIAIRTDVTSIAHILNSHGYRCGYIGKWHL
                     GGIPRYKFTPPGPERLGFDAFWAVWNCHHDYMNPRYYLDTPEPVVMEGYEPSVQTDLA
                     MAFMDRHREQDNEHPFCLFLSWGPPHSPYEPLPPGRETQYDPSALVLPPNCPDTPENR
                     RHLAGYYAHISALDAETQRLLDYLRANNLEKDTLVVFTSDHGSMLGSQGHTHKQRPWA
                     ESVVVPLIFYRPGAVPEGRVNKTLFSLLDFVPTLLGCLDLQVPPSMHGEDLSACVRSG
                     AMKEGRVLYLADLITTDQANHYGIKPWRGIKTERYTYACDTQGEWLLYDDERDPYQLE
                     NLAGDPLAAETRASLHQELQRQMERFGDRIMDVAEALEAWGLTEAFRIRTDHLYASPH
                     NMGGRLPQ"
     gene            complement(33315..34421)
                     /locus_tag="PLT86_00490"
     CDS             complement(33315..34421)
                     /locus_tag="PLT86_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT35126.1"
                     /translation="MDERPGADFSSVAIVGVGLIGGSLALALKEFGATGRVVGISSRK
                     TIKEALGLGAIDEGWEYNDLALALRETKAVFLCTPVSRIIELLPEVAHVITPGTVVSD
                     VGSTKKEIVERAEKCMPQHSVFIGGHPMAGSERNGVGAADPFLFQNALYVITPSGSVP
                     ESVRASFTERIASIGARVFCLSASAHDRVAAAVSHLPQMIALALVEYVGTLNAIEPAH
                     LQMAAGGFRDMTRIASSPYSVWQDILATNASEIRKALRGFAGQLEQVLADIEHMEPHF
                     RAANEIRSSIPRDTKGFLSPLCDVLVRCEDRPGQLARMTAALAREQINIMDIELLKIR
                     EGEGGTFRMAFKDIQTADAAIRTLDKEGFPARLR"
     gene            complement(34424..35512)
                     /locus_tag="PLT86_00495"
     CDS             complement(34424..35512)
                     /locus_tag="PLT86_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35127.1"
                     /translation="MEFRCKDDWEKARTRIEAWWNNEIIDRAVVAVTAHNGRPRKEIP
                     TPASMVERWTNADYLIARQKAHAETTYYGGESFPLMFVNLGPSVGSAYLGCELAFAET
                     TAWQESPVITSWDTWLPFCFDSQNLWWRRTLAITRQAAEELEGISFVSITDLGNSTDI
                     LAHLRGPQQLLLDTLENRYDVEWALAQITSLWFQWYEELLAITRTRQEGSCGWLGAWS
                     PKRTYPLQCDFSCMISPQTFRWMVMPEMERFVGRLDHPIYHLDGPNAICHLDALSQLP
                     HLKAIQWVPGDGNPSARYWPDLLKRILAMGKGVHVYVPAEDVEPLIDEVGPKGLFITT
                     GVGTPEEAVSLLRRVEIATARAAHRGVA"
     gene            complement(35572..36588)
                     /gene="aroF"
                     /locus_tag="PLT86_00500"
     CDS             complement(35572..36588)
                     /gene="aroF"
                     /locus_tag="PLT86_00500"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOT35128.1"
                     /translation="MVVAMEQWATEEQIAAVIARLQEHKLDVHRSTGTSRTVIGAIGE
                     THMLDLQAFEVMPGVAEVVRISEPYKLASRTFKREDSVVYVGDVPIGGKEIVVIAGPC
                     SVESLDQTRRTAERVAAAGARMLRGGAFKPRTSPYSFSGLGEKGLEILRTVADEFGLK
                     VATEAMDRTQIDSVVAYADMLQVGARNMQNFTFLRELGKTRIPIMLKRGLSATYEEWL
                     MSAEYIMAGGNHNIVLCERGIRTFETYTRNTLDIAAIPVIKKLSHLPIIADPSHGTGR
                     RDKVIPMARAAVAAGADGLLVEAHVDPDHAFSDGVQTLNLDQFDELMGQLRLITPVLG
                     RTIR"
     gene            complement(36639..36935)
                     /locus_tag="PLT86_00505"
     CDS             complement(36639..36935)
                     /locus_tag="PLT86_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT35129.1"
                     /translation="MDIADWRDRIDDIDRQLVVLLNERAKCSLNIGKLKRRMNLPIYD
                     PRRESVVLQQVVDLNEGPLTADAVKRLFERIIDENRRLERIYSALEESLQGNAT"
     gene            complement(36938..37771)
                     /gene="trpA"
                     /locus_tag="PLT86_00510"
     CDS             complement(36938..37771)
                     /gene="trpA"
                     /locus_tag="PLT86_00510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT35130.1"
                     /translation="MNEYNHDHCGSSRIHRAFASAKEHHRPAFIAYITCGDPTLDRTR
                     QLAVEFAGAGVDILELGVPFTDPMADGTANQEAALRALAKGVTLRHVLRTTEALRGEG
                     FGLPVVLFTYYNPVFAYGVEKFAVDAVSAGADGVLLVDVPVEEAQEFKPAFDGAGLGT
                     IFLVAPTTDDRRLPHILELANGFVYCVSRSGVTGERNGLASGIRENVARVRRLTQLPV
                     AVGFGISSREQVSEIGQFADGVVVGSAIVRRIGEGGDTDEMVRQVTEFVRTLTGQFIG
                     G"
     gene            complement(37768..39039)
                     /locus_tag="PLT86_00515"
     CDS             complement(37768..39039)
                     /locus_tag="PLT86_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOT35131.1"
                     /translation="MKCGVATRCINPERPVRMAGYASRDKDSEGAYQDLNLKALALGD
                     GNATVVIVTSDILYFDDDVIGPVEVELARNPGIPPDRLFITASHTHCGPVTRRGDRDM
                     YGWRDEEYLDQIRQKLVESVREAVAEMAPCTLTYHRGRCGFNINRRVKTPSGVQMRPN
                     RDGAVDRDVDILCTRTSGQIAAILFGYCCHPTTMGGYLLGGDYPGFAKIGIESALPGA
                     TALFVQGCSGNVRPNNVDETGNFKSGPLEVVQKFGEELAEAVLNVVAEPGAAIEGNLK
                     TGARILELPLMPHPSRAELARATEDGSPFRRRWAWRMLDAYTRELPLPTTCSVHVQTL
                     QIGNALSLVAIAGEVCVEIGLRIKSLIGDGPRFVLGYTNRVIGYVPSKAIHADGGYEV
                     DGWYFYENMPSYLAADAEDIIVGAVGEMLAE"
     gene            39166..39525
                     /locus_tag="PLT86_00520"
     CDS             39166..39525
                     /locus_tag="PLT86_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35132.1"
                     /translation="MAGDHMDRFFGNDNDRPDEWDGEDNDFSDEDAEFPQSGLEIAIS
                     PFLGYEVELQGTFPIQLQIIRPATPNSARVLAGVYPDCIVVFDQEHYLVLPQTMVIVS
                     VPRVEWESREPLEPGDD"
     gene            complement(39558..40403)
                     /locus_tag="PLT86_00525"
     CDS             complement(39558..40403)
                     /locus_tag="PLT86_00525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT35133.1"
                     /translation="MSTGRHTCVIRSRLKEISFDHIYFGTLFTWFEVARTEMGRAAGL
                     PYVELEKNRIGSFVTAAGAQYHRPIPAGATVAVEARMAEMGRARCSFAYAVFTGNDES
                     PAVTGYTDHIAADFSGRPTRLPACFAEAFAPTGESIPRPSLEEPARVHWNTGLRVRYE
                     ETDAFGVVYNGNYFAWMEAAWSEHLADNGMDVVESFRKGQTYPVVEASCRFLSPLRYD
                     DPVSIEVESRLEGRTRVALRYRFVAASAGITAGIGQMVHAVVDKGRVVPVPRELAHAL
                     GITDS"
     gene            complement(40400..41596)
                     /locus_tag="PLT86_00530"
     CDS             complement(40400..41596)
                     /locus_tag="PLT86_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35134.1"
                     /translation="MTGLIVLFALWVVLLTGVWVHPRLSRKAKIVALVPIVAYGIISG
                     VSVARSYAAGRPQFSRVTQKWALAEEHLASVLGSAPDSALAKPLVQSAKKLHADLKRT
                     MRFGVWSWGVRYRFGIIPQVNGDVVAANLERLLDVPAQLVYAEAANVRNSRDFTQCYD
                     YRLLVTRDFPMNYSRSAGVVSMILEAYKSGATTAIGLCDTVNLARDDARDPRSPTYRL
                     SAVAVKLVGMQNKLDELLAVACGAFDEEIALSVRNVRPPDISQSPEWKPLSPLFMDFT
                     PLVPNLTGISTIEVRWVGDPPKPVVGTTEPMYRLDCETQGTWPAGQDIWRDSTGSSGP
                     TYFVDASASRVFAKGVRLLKWDRQPDIRSLNIMANAEDRRYAGIPTVDVTEDLFLPPP
                     DRSKRR"
     gene            41821..42264
                     /locus_tag="PLT86_00535"
     CDS             41821..42264
                     /locus_tag="PLT86_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOT35135.1"
                     /translation="MSALKGIPMSISPELLEVLARMGHGDHIVLADGNFPGESQGADY
                     VIRVDGMGVPALLKDLLKLMPVDTGESSAYVMAVPKGQPEPPIWAKYREIINKAEKRE
                     VKLVELDRFAFYDFTKEKAFAIVMTGETALYANLILVKGVVAPRK"
BASE COUNT         8423 a        12096 c        12849 g         8768 t
ORIGIN      
        1 ggcgcgcgtg cgttttcatt tctgctctgc cctgcgtgtt gtgcctccgc tgaaccattc
       61 aaacgagagc acaagaacga caaaccattg gcctcgatgc ctatcgtaat gtaggttcga
      121 acacgtaaga agtctagagc gaacgtgttc cgcaccctga cacacgcgcg agggtcttcg
      181 agacccgaag gaaggcaggc accatgaatg aagacaccgt gagcggggat ttcctcaaac
      241 cgtcaaaaag gacacctgtg cgcgtaaccg tcaggaatcc ggaacgggaa aaagccggtc
      301 aactgacacc gaaggaaacg gaaataccgc gtcttgcgga ggaaaaggca ccgcagacac
      361 gggttgtgta ttcaacgcgg gggtatgagt atgaagcccc gttcaattat cccaatgtca
      421 actgcgaact gggccgtttc ggaacgggaa caggcgccca cccggacgga atggagctgg
      481 acatcccacc tttcggcgcg atcacgatcg aagaggcgga gccgatcatt ggagtgaccg
      541 taatcgggag cccgtgcatc cccccgggaa ccatcctggt gttcggcgaa cctctcgagt
      601 ggaagtacgg gcgttcgggt gtgaagttca aacagacgaa tatctggggt gagcacctgt
      661 accgctgggg cgcaaatccg gagttcgagg aaggaaatcg gcacgcgggg ctggccagcg
      721 tgcacttcga aattccggat tgccgcaagg tgacggtgat gggatacggt cagcttgacg
      781 tgcacgtaag ccctcccgaa tggacgcccg gcaaaggccg ccagggactg gagcaatacg
      841 agatgcccgg tgaagactgg tggaatgacg cacattacaa cgtgcggacc atccgaatta
      901 cggtgcgcgt gcctgcctga tatgggcgcg gcacgcacga atcgcgatcg cgatagagag
      961 ccggttcccg gtccgttcat tgcccattcg cggccacggg cttacctttt ctgcggggtt
     1021 ttccgcttct gtgaattctg ttcgggccgt ccatgcgagt caatttcctc acggtagggt
     1081 gcaaactcaa caactgcgaa accgaggtgc tcgagcggca tttctcccgg gcgggctggg
     1141 atgtggtttc ccgcgaggag ccttcggacg tgacggtggt aaacacctgc accgtcacgg
     1201 cgcggagcga tgccgattgc cgcaaactca tccgacgcgc gaaatcaatg aaccccgcgg
     1261 gcagggttgt ggttaccggt tgccttgcgg aaaggacccc ggacgcgctg gccggtatgc
     1321 cggaggttgc agccgtaatc ggaaaccgcg cgaagaaaaa cctgctttcc atcgtcaccg
     1381 ctgaacttca gaaagcctcc ggccctttga tcgcttcgca ggatcagagc gggccgggtt
     1441 tcctcccgct ttcgccccgg gttggatctc tctcagcgac gagaacacgc gcaacggttc
     1501 aggtgcagga agggtgcgac gaacactgca cgtactgcat tgtcccgcgc gttcgcggtc
     1561 gaagtcgttc aagaccgatt gaggacatcg tcgcggaaac ggagactctg gttgcaggtg
     1621 gttccgttga gattgcgttg acgggtgtca acacgggtgc ctgggggctc gacctcggag
     1681 ccgaaggggg catcgctgcc gtcgtggacg ccatccttcg cgttcacact ccgtcgggac
     1741 agagggttga acgccttcgc ctcaactccc tcgaaccgca ggcgatcaca ccggatctgg
     1801 tgcgcaagtg cgctatggaa ccgcgtgtat gccgccattt tcacgtgcct ctgcagagcg
     1861 ggagcgatgc gattctcaga aagatgaacc ggcgctacac tgccgcggaa tacgcgcaga
     1921 agatagagct cattgcggaa catattcctg acgtggcaat tggcgcggac gtgatggtcg
     1981 gattccccgg cgaaacggat aagcagttcg gcgaaacctt tgaactcatt cggcgcttgc
     2041 cgctcacgta tctccatgtc ttcccctttt ccccacgcga gggaacgcca gctctccgac
     2101 ttggccctgc aatctcctca gagcggaagc aggaacgcag ccgcgcgttg agggaactgg
     2161 gaaacaggaa gcgccttcag tttcacctgc gacacgtggg caatgtcgtg acggttcttc
     2221 ttgaagggaa accagtgact gccaccacgg tgcttagtgg attgaccgac aattacatcc
     2281 gtgtcgccgt tcgggctcct tcagatcgca tcaacacgtt ggtgcccgtg tatgtcgaag
     2341 acgcttcccg ggagggggtg cggggcgttc tggtggacga atgctccacg tcattttcac
     2401 ggactcgagg gagtcaggaa tgagagtccg tcaacttgac gtgctcaatt gcgacatcgg
     2461 cgcgaacgag tcatccgcga gtaccagtgg gcacatgcga gtttttttgg agacgtacgg
     2521 gtgccagatg aacgtggcgg attcggaact catcaccggg atactctccc gagcgggttt
     2581 ccagatggtt tccggtccgg aaagtgccga cgtgattctg ctgaatacgt gtgcagttcg
     2641 agagaacgcc gaggagcgcg tatttggcag gctgggagag cttacgcagt acaagaactc
     2701 gcggccgcac gttctcatgg gagtggtcgg ttgcatggcc gaacacctgc gggaagaaat
     2761 cctcgggcgg gcgaaagcgg tggggctcgt tgtcgggccc gactcgtacc ggaggcttcc
     2821 tcatttgatc gaatcagcgc tcgccggccc ggtagtcgat atcacgctgg ataagagcga
     2881 gcagtacctc gggctggagc ccgatcgcgg tggaggtgtg actgcgtgga ttccgatcat
     2941 gcgtggttgc aaccgcttct gcacgttctg cgtggttccg tatacgcgcg gcagggagaa
     3001 aaatctcccg ccggaggaaa tcgtacggca ggtggaacac gcggtgaatc tcggacacag
     3061 cgaagtatgc ctgttaggcc agactgtgaa ttcatatcgc cacggagata ctgactttgc
     3121 cgacctggtg cgcatggtgg gcgcggtgga cggaatcagg cgtctgcgct ttatgagccc
     3181 ccatccggtg gggttcactg catcgcttgt ggacgcgatt gcggaggttc ctgccgtatg
     3241 tcctcacgtg cacctgccgc ttcaatcggg ttctgatgcg gttctgacgc ggatgcgcag
     3301 ggattattcg gcgcaggagt ttcgtgcggt gctgtcagac ctgcggcgcc gaatccccgg
     3361 aatttcggtt tcaacagaca ttgtggttgg tttctgcggg gaaaccgaag atgaccaccg
     3421 gagaactgtc gagtttgttg aggaaagtcg cttcgacttc gcgttcacat tcatgtactc
     3481 gccacgggcg ggaacgttcg cggcgcggtt cctcaaggat gatgttcctg cggaagtgaa
     3541 acgccgcagg atttcggaag tgattgcgtt gcaggagcgc atttccgccg atatccatgc
     3601 gaaacttgtc gggtcggtca gggaagtgct gttcgaaggt tccgccaaga agggggatgc
     3661 acagagcttc ggtcgaacgg ccgatttcaa aggggtggtg gtggaaggct cgtttgctcc
     3721 gaattcgttc cggtccgttc gaatcacggg agccaacgcc cacacgctca ccggcgaggt
     3781 ccttcccgac acaggagcac ggccgtgatg atacctcgcc ggacgcgggt cacgatagaa
     3841 cttgcggcgg ccgctctctt cctgtgtgca aatcgggtcc ctgcggcaac ggggacggtc
     3901 gtggtggttg ccaacggcgg caatcgggtt atcagttcca gcgtggagca cggggttacg
     3961 ttcgtaagcg tgccggacct ggcgcgagcc ctggagctgc aaaccgaatg ggatgccaga
     4021 actcggacgc ttgttgtcct ttcgggcgaa acggaagcgg aattcatcgc gggcaatacg
     4081 cttgtggtgg tgggcgactc cacctttcac cttcgccacg gcacggttct ccgggcgggg
     4141 gaacttctcg caccggcaga cgccctgccg gggctgttcg gtctcctgtc gccagtagcg
     4201 cttctgtggg acgaccggga gcgtgtcttc cgcacgtccg cggatttcgg ctccggcgcg
     4261 ggtgatggag tcacggatga cacacatccg aatgggcgca accgatggga gatcgacaga
     4321 gtcgtcatcg atccggggca tggcggacga gacccgggaa cattgagcgt ggagggggtt
     4381 ccggagaagg aaatcacgct cgctattgcc cttcgacttg cgcagcttct gcgagacgaa
     4441 ctcggtgtag atgtcgtaat gacccgaacg gatgaccagt cagtcagcct tcgggaacga
     4501 ggcctcgcag gcgttcgcgc gggcgggaag ctgttcataa gcatccactg taacgcgatc
     4561 gacaaggccc atgtaagcgg agttcagacg tactttcttt cggatgctga gacggatgag
     4621 gcccgtgcag tcgcccgtgc ggagaacgcc gcgctcatgt atgaggagca cgcggacact
     4681 ttgagtgaag gcgacgatcg ggaaatggaa gagattctgg ccggattggt ctctgatcgt
     4741 tttctgaagg agagccagga actggccgcg ctggttcaga gggaactggt gagaggtctc
     4801 cgtacccggg atatgggtgt gcatcaggcg ggcttctatg tcatgaaggg aacgctgggc
     4861 gcgatgccga gcatcctgat cgagaccggc tacctcacca atcaggatga ggccgcgaga
     4921 cttcgggatg cgcgatacca ggagagaatc gcggatgcga tactccgcgc ggtgcgtgaa
     4981 ttcaagatgc gttatgaaac tgaactcggc aggtaggagc gaataatgga caggcgaccc
     5041 ataggtgttt tcgattcggg ggttggcggt ctcaccgtgg tgaacgagat ccagaaacag
     5101 ctgcccaaag agcgtgtagt gtatttcggg gacaccgcac ggttccctta cggttcgaaa
     5161 tccgtggaaa cggttaccga gttcgcccgg caggattcac aattccttct ggggtttgac
     5221 gtcaagctca ttgtagttgc ctgcaatacc gccaccgcgt gtgcgctgga tcatttgagg
     5281 gagcatctgt cggttccggt tgtgggagta atcgagcctg gagcggtggc tgcggtccga
     5341 gccagcagga ggaagttcgt aggcgtaatc ggaacccagg gtacaatagc ctcaggggcg
     5401 tatcagcgcg cattgacgcg gatcgaccct tcgatcagcg tgttcgcgca ggcctgcccg
     5461 ttgttcgtat cgcttgccga agaagggtgg acagaccgcg cagcgagcct tctgatcgcg
     5521 catgagtacc tcgatccttt gcgagaggcg atgattgacg tgctcgtcct gggctgcacg
     5581 cattaccctc tgttgatgcc tgttatccgc cgtgtcatgg gaccggaaac catcctcacc
     5641 gactccgcgg ctgaggttac ccgggaggtg gggcgcgtac ttcaggaaaa cgaactggaa
     5701 agcgacggga gtgcagtttc tggccatcgc tttttcgtgt cggatgcacc ccaaaaattc
     5761 cgcgaggttg gcgaacgctt tctcgggcgc gcattgccca ccgtggagct ggcgacggcg
     5821 ggctgaacgg acggcattac gtgtggggct tgaccgtagt tggaaccaac cacgccggga
     5881 gggccggatc ggagttcgcg aatacctgcg aaagggaata ctatggaacg gaaagatttt
     5941 ctgaatcctg cgggaatcta ccgccccgcg ccattctggt cgtggaacga cgatctccgg
     6001 aatgaggagc tggaagcaca ggcgcttgac atgaaaaaac gcgggtgggg cggttatttc
     6061 atgcacagcc gtatcggtct ggtaactccc tacctgagcg acgaatggat ggaccggacc
     6121 cggcacacgg tggaactttc cgcccgggag ggtctttgcg cgtatctcta cgacgaggat
     6181 aaatggccat ccgggtacgc gggcggactg gttccccgat cgaatcctgc agtgagaaac
     6241 accgcgctcc agtgccatcc ggtgccccct ccagaagaca ccaataccgt tctcgcggtc
     6301 tttgccaggg agtcaggcag atgggttcgg atagaccccg cgtcagttcg ttccggaacg
     6361 gaactggcgt acatcagcca gtgggtcgag ccgatgggga atccctggtt caacgggacg
     6421 acctacgtcg atttgatgaa tcccgaggcg ttcgaggagt tnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nggttcaacg ggacgaccta cgtcgatttg atgaatcccg
     6601 aggcgttcga ggagtttgcg agatcaactc tcgacccgta tgcggcgctt ctcggggcac
     6661 actgcgggac gaccattccg ggctgcttca cggacgagcc tgcctacatc ttctggcatg
     6721 cacggggaat ccacccttcg aacaccgtac cgtggtcacc cggctttaga gaacggttca
     6781 ccggaagatg gggttacgac atactcgatc gaattatctc ggttttcaag cgcgaggggg
     6841 attttgcccg cgtgcgctac cacttctggc gcacggcgac ggaaatgttt ctcgacaatt
     6901 tcagtgagcc gtacggacgc tggtgccgcg agcacaacct tgaattgacc gggcactaca
     6961 tgtgcgagga caccctcgtc gaccagattc agtgggtcgg tgcagccatg ccgcattacg
     7021 aacacatggg ctggcccgga atggatcatc tcgggcgcaa catcgacaac atcatgactg
     7081 cgaagcaggt tacatcggtc gctcatcaga tcgggaaggg ccgcgcactc tctgagttgt
     7141 acgggtgctc cgggcagaat ttgacgttcg aagggcgcaa atggatcgct gactggcact
     7201 tcgtgcacgg tatcaacctt ctgaaccctc accttgcgtt gtactcgatg cggggcgagc
     7261 gcaagcgcga ttaccctccc acaatctcgt tccagcaacc gtggtggcgc ttcaataacc
     7321 tcatcgcgga ttacgaagcg cgattgtcgt acgcgcttac tcgcggaaaa cggatttcgc
     7381 aggtcctcgt cattcacccg atcgaaagtg cgtgggccct gtacgaaccc ggggatgacc
     7441 gacgcgcaag gcaattgagc gaatggtttg accaggtcag ccgttggctg ctggaagagc
     7501 actacgacta tgactatgcc gacgaatccc tcctggcaaa atacggcaga ctggagcacg
     7561 acaggctgtt tgttggggac gctgagtacg aactggtggt gatcccgcca tgtttcacgt
     7621 tgcggcggac gaccgttgac ttcctgcaga agtggatggc ggcggagggc gcggtgatct
     7681 caatgaagcc tgtggcgcgc aggattgatg cggaaacgga cgcgtgggat ttcgttagag
     7741 ataccatcgt cgtggagaat gaccgaacgg cattcacgag cgttctcggc gagcttctgg
     7801 agccgaatgt tcgcattctt tccccggaag gttaccctgt agcgcccgtc tgggtgcacc
     7861 agcgagcgga cggggagaaa gacctctttt tcttcgccaa cacagaccgc acccgtgggt
     7921 acgcgtgcga gattctgctg cagggagatg gggcagtgga ggaatgggac ccgcgtaccg
     7981 gggacgttca tgaggttgcc gtcgaggtgt tggatgacga ggtcgttgtg agcaggttca
     8041 ttgaaccctg cggttccctg ctgctggtga tggatcgatc gaaggatgca cgcgagatcg
     8101 atgaagacga ggaggaattt ttcgaagagg gcgatgagat tgaccttgat ggcagctggt
     8161 taagcgaacc gcgtgatccg aacgcgatca ccctcgatac ggtccgctgg agacttggcg
     8221 acggggagct tcaagggccg gacccgctct ggaaagttct ccatccggtc cgaagccatt
     8281 cggggacgtt ctcactggag ttttgcgtct ccgtggaggt tgttccagag tcagatgtgt
     8341 ttctcgttct agaaacgccc gaagcgtttt cggtcagtgt gaacggtacc gaattgccgc
     8401 aaaccgactg cgggttctgg gtggatacca gttttcggaa acgctcattg aacgggcttc
     8461 tgagaatggg cgagaacacg atagttctga gcggcacggc cgacccgaag atcgagctgg
     8521 agtcacttta tgtgatcggt gacttcggtg tgaggacaga agacaaccga gcctttgtga
     8581 tcacgtccag gaagtctgtg gttcttgcgc agaacctagt tgaggaagga ttcccgttct
     8641 tcgccggaac gataagcctg acccagtcct tcgaaatgaa cctttccgca tccgggcagg
     8701 cgaagctcgt gttcgatgat cctcagttcg ttgtggccga cgtgtgggta aacggcaaca
     8761 atgcgggaca ggtggtgtgg agcccgtacg aagtggaaat cgggcgcttt cttgttgacg
     8821 gaacgaacac catcagggtg gatctggtca attcgttgcg caacctcctc gggccgcatc
     8881 atcacgcatt cggggagctt ctcggcgtgg ggcctgacag cttcagcgat gcagagaact
     8941 ggaccgatgt gtaccagttc gtcccgtttg ggtttggcgg agcacgggtg atcgtgcagt
     9001 cgtgagcttc cgggtcgccg gtgaggggat tggcggtggc tccggctgcc gccagcacaa
     9061 gccgtttcca gcctacctgt tcgcctccca gggagcgaaa aggtctcgaa atgttcgtgg
     9121 ttcctcaggg gtgtccgggt ttccttcgcc ttccccccgc ctcatcgcgc gtaacagctc
     9181 tcgtcgtccg gtagccgcga atacggctgc cacagacatg gcgatgagca acaagccgac
     9241 ctgggtcaat gccatcgcgg cgcccagaat ggcggttgaa atgagcaggc cggcggaacg
     9301 agcgggatat cggaatgtca gcaggaacga atcgcgaacc acctcgcgga acctggctcc
     9361 gtcgtcgccg gctgcgcggt tcaaaccaaa ccagaacgcc accagcagga acgcgatctg
     9421 cgcccacagc attactcccg ccagaagcag gccgacaaac cgtggcatcc acgacgcgcg
     9481 aagatagaac gccgcatttg cgccgagaat aacgaagacg agagcggcgg cgatgggcag
     9541 cgcgattccc ggaaccaatt gagacctgaa aaatctccac acgtcagccc gctcgacgcg
     9601 ctggtaacga acccacctcg tggcgcagaa catcgcggtc aatgttgccg gattgagcca
     9661 gatcgcggca aacccggctg cgagtgccgt cacaaatccg actgcaccaa agctcgaaac
     9721 cagtaatctg cccgcgctcc agatcgcaag aaaccaggga agacttgcca ggaaccacaa
     9781 cagattcagc acaacacacg tgccgagatg gtcgtaaagg ttccagaaga acttcttcag
     9841 catgcgttct ccgtctggcc actgccaacg tggaggcgtt tctccggtgc aaatgaacca
     9901 attactttga aagataggcg catctgctac tgcgagatca ggtggtcatt ttcccaaagg
     9961 atttggcaaa tgttcgtgga aaacgtggtt tatcgtcccg acagcgaacg tggcgtgctc
    10021 gacatcatgc ttcccgaagg ggcaggccct ttcccggctg ttctgtgtat ccatggcggc
    10081 gcgtggtcag gcggggacaa aagcatgatg caccctttcg gggaggagtt tctgaaactg
    10141 ggaatcgcat cgctctttcc caattatcgg cttaccggaa cccacccaca cccggcgcag
    10201 gaacaggaca ttttcgctgc gctggaatgg gcggcggcca acgcggagag gttccgactc
    10261 gacgtgactc ggttcgggat caccggcgcc tccgcaggcg ggcatctcac cgcacttgtg
    10321 ggcctgaaag ccacgaagaa gagggacatc gggtacacgg ttcgatgtat ggcaccggtg
    10381 tgcccaccca ctgacttctt cgccttttcg agggacaatc cgtccttacg ttcggtcctg
    10441 gaggcccttg tgggcggcga gctggaaaag aacgaagctg taatccggga cgtgtcgccg
    10501 attttccatg tgcacgaaaa agcccctccc tgcctcgtaa cgcacggcgc gattgatagc
    10561 gtggtgccgc cggcacaggc tacctcattc gttgatgcgc tcaaatccgt gggagtggaa
    10621 gcagaggtga ttctggtccc cggtgtggac catgcggcgt tcaatccggg tactgatccg
    10681 ccggaaccac ttggcggtat caaagcgttc cgtgcgtttt accggaagca tcttctctcc
    10741 tgaagccacg atcgcgtttc acgtgcagcg cctgaccgcg aggagtctgc agccaaatga
    10801 atcctgacta ccgttttcac ggacgaagca tgaccttcat cactgcgcca gatgcgacac
    10861 aggcggaacg acatgtggca cgggtggctt cggaagccgc gcgtcgtcgt ggggtgaccg
    10921 gtcattgcca cgaatccgga gacgttcctt cgactgatct ggccgtcttc ctgggtgtgg
    10981 gagaaaccca tccgcatgtg gtagaagaac tcagccgtcg tgatcgcttc gcgccttcat
    11041 cgccgctgac gcggggcgct caatcgagca gaattgtctc gtgcgtgaac cagagcgcag
    11101 atgcagttgc catctggggg gcggacatcg aaggcctttg ggttgccgtg gggcgctttc
    11161 tgcggggctt gcgctggaag gacggtgcgt tttcgtcagc gggatgggat gacgagtttc
    11221 gcccggcgtt tgaattacgc ggggaagtga tgtccagcca ctttaccgcg aacacgtttt
    11281 tccagtggtc gccgcggcag tggttcgagt atatggacga gatggccctc tggggcatga
    11341 atctattcat tacgattccg atacacatgg cagactggga cgggatcagt ccttggtctg
    11401 atcctccgcg atgggtgtcg gaggagcgcc ggaaagagtg ggacacgcac tgggaaacac
    11461 actgtgcggt atcgcaacgc gtccatgaga tggggcttcg atacggcctg tggattccgg
    11521 cgaacgatgt gccgcccatg atggcagaag cggactggaa ccgcggcggt ggcaagtacg
    11581 tgtgcccgtc cgttcccgaa gcgcggcggg cgatccagta tacccggagc agggtgattc
    11641 agggactgcc gcacgtggac tactggtttg tgccgtcggc ggacaacggg gggtgtccgt
    11701 gcgatgattg ccagccatgg gttgacacat acctctcgct tgctgaggag caattccggg
    11761 agctggcgaa gtggcatccc ggggcaaaag tcattctttc caatcaggct ctcactcacg
    11821 aggaaaacaa gaaactctgc gcgcggcttg cggagccggg aaccgaatgg atagagggcg
    11881 tggcatatgc gccgggatca aatgagacct acatacaggc tcgcgagacg gaggaatatg
    11941 actggatcga ctggtatcag tttgatgcgg agcatcgaag ccttaaagag ctttcacgca
    12001 ccatgccacc ttcaaaaaag ctgttcctgt atccggacat cacccatctg acgcgttcac
    12061 agtacccggt gtgggatgtt gacccgctcg tggcatggac ctaccaccgc gacacggtgt
    12121 tctttcgccc gcgggggtat gcggaggtgt tcaggcgctc agcgcctcat gcggacggca
    12181 ctttcccgta ttcagaaggg atttacgatg acctcaatca ggttgtctgg ctcacattga
    12241 gttgcgaacc cggtcgtacg cacgacgaca tactctctga atactggaga tggcatgccg
    12301 gggaggatgc ggtcttcccc gccgtgtgtg ccacccatca gttggaagat tcgtggaggg
    12361 gagatatcac ggcgaacgcg caggtcgaga cggcggttct ggaaaccgaa aaagcagtgc
    12421 gcctcgcccg cggaacgggc tggcgacttg atcttctgag gatgcgggcg cttctggatg
    12481 agtggctgaa aaaccggctt cgtgccgcga aactgttgga aagtggaatc atcgcaatgc
    12541 tccgggatga cgccacggag agtggggtgc tgcgcgcgat cgagtcactc gacagcgcga
    12601 tacgttccct ccccgaggct tcggatgagg tcctccgcct gggagaatca ctgcttcgcg
    12661 cggggccgag aatgccggtt gggcgtgtcc gccacgtgac gggtaacctc gcctggatga
    12721 aggcgactct caaaaaggcg cttgtggatc cttcagttcg ttcggcgctg gtggcggacg
    12781 tgatcgacta tgaacgcggg gtttactacg tggattgcgg gaaccccgtt cgggacacgt
    12841 tttgcgtgca cggtcggagg tacacctgtc tggatgaacg atcctaccag acaccgttct
    12901 ctgaagcacc ggaaatacga ccatcccaga actccctcgt catgacgtgt ggtgaggacg
    12961 acggcatcgt gtatagattc cctgatctgc acccggatcg gaagtacgtg gtgcgcctca
    13021 cgtatttcct tcccactctc ttcacgtatc gagagacgcg gagccagaaa cttgaggcca
    13081 acggcgtgga ggtacacggt ccgttggttc ttccacgaaa acgtccggaa acattcgagt
    13141 ttgcgttacc tcgggcctcg tattcagcgg gggagacgac cctgcgtttc aggaaggccg
    13201 agggtggagt tacgacagtt gtttccgaga tctggatacg cgagtgcgat tgaaatcggc
    13261 gaaaaccaat cacgagggtt cccatgccga ccactgttgg tcgtattgaa gagactgtcc
    13321 ttgagaagta cggtgacttc cacaacggcg ccgggccaat gtggtgtttc ggttctcaca
    13381 cgatcgtcag gtcgggagat accgtgtatg caagcatggt ggatgtggag ccggaagcac
    13441 gtcccctgtg caacgccaaa tggcaattgt ggcagcgcca ggacggcggt tcatggggaa
    13501 aaatagcaga acaacagaaa gccggtgaac gagagccctg cccgatcgcc ctgggtccgg
    13561 gtggcacgct gcttctgtcg gttagcactg ctgtttcacc cagccacgat aaccatgatg
    13621 gaacaaccgg ttttcactgt ctcccgcatg tgctgagttt caggatcgct ggcggcatcc
    13681 ttccggagcc aaaggtgata attccgcagt ggaatgcgga atacccgttc aacgaacatt
    13741 cgtaccgcgg attttcctca gatgcatcgg ggaatgtttt cctcacgcac caggcgtcgg
    13801 atgggtctga atacgggcag gcatggtgtt accgaacgtc agagggcgat tgggtggcag
    13861 ccgggttcct tcgcttcccc atgcgtgggt gctaccccgc ggcggcggtc aacggcaaag
    13921 cagtcgccgt gctcgcgata agtgatgaaa tcgagccgaa cgaggaatgg cgctctttca
    13981 aaaaagagaa aaccggcagg gactgggact atgatttccg gcagctgttc ttcacatgga
    14041 cgccggacgt aacagcggtc cccttctctg tgcccctcac cattgcgtct gtggacgaga
    14101 cctgcggggt gatcactcac accgaccttc tggttactcc tgacggagat gtgcacgttc
    14161 tttacatcga gcggaacgtg taccacgatt tcgtgcgcga gcgcttcttc cccgatctcc
    14221 cactgtccgt ctccctgaag tacgtacgat tgcgccgcgg gtgtgtggtt gagcgccgaa
    14281 tcctctgcaa atcctgtgag aagtttgaca acacccgggc ggagaccgcc gtcaaaatgg
    14341 gcgcgccagt acccacctgg gcggtatttc atgcaaccgg cgccgggagg gttttcctgg
    14401 tctggaatca gacagacaac gttgcgagcc agaacggcat gttccttcgc cagatcatcc
    14461 ctcaatggga tacaacagcg atttccctgc cggtgagaac cccgttgacc cagttctttg
    14521 ccgcctccac ccgcgccggg tgccttcctg acaaccggct tgatttgtac ggaacggctc
    14581 ccggtgaaca ggtcatccgt accatccatt gcgttgtgga aaccgatcag gcggaggtta
    14641 cgagatgaca acgccccgag agcgcgttgc acgcgccgtc aatttccagc cgacagaccg
    14701 tgtcccgatc gaccttggcg gcatgaaggc cacgggcatc gcggtcaaag cgtacaacgc
    14761 cgtcaagaaa cagatcggat tgtctagccc gacgcggatc tgggatccga aattcatgat
    14821 cgcccacgtg gaagaggaaa tcaggcagag attccatctg gacgtcgtcc cgctggacgt
    14881 ttcctctgta tcagatgaga tgcgtccaca acgtgaatgg gtgccgatgg cgctttatga
    14941 aggcgcggaa ggtctcatgc cgcccgggac aatgatcgga gaggatgacc ggggacgctg
    15001 ggtgctcatg ggcgcggatg gacggccgac gacgttgcga atgcctcatg aggggtacta
    15061 tttcgacgac atatcgttcg atcgccccgg aatggaaatc cggccgtccg agttccaacc
    15121 ggaagccacg ttttcggatg aacagcttcg cgcagtggag gcgagagcgg acttcctgtt
    15181 ttcccagaca gactacgcgg tcttgggctg gggaggcggg gtgtgttttc tcgggttgag
    15241 cctgatcacc gacagatcca gtaacgtgac gatgaattgc cccacccagt ggatgaccat
    15301 gcttatcacg gaaaaggaac tgtgtcacga aatgatgggc cggtctgtgg atgcggccat
    15361 ttcacggctc agtctgcttc acgaggccgt ggggaaccgc tgtgtcgcgt gggggattgc
    15421 ggccgacgac gccgggactc aacgcggcga gttcatccgc ccagacctgt gggcggaaat
    15481 gataaagccg cattacgcga ggctgtgttc gtgggtgcac tcgcacaccg aatggaaaac
    15541 attcctccat tcgtgcggtt ccgtgtatca cctcattcct cacatgatcg aggccggagt
    15601 tgatatcctg aatcctgttc agacctcggc ggcgaacatg gaaccgggac gattgacgag
    15661 tgagttcggt ggaaaactgg tgttctgggg tgggggatgc gatacgcagc atgttcttgg
    15721 caacggctcg cctgaagagg tacgcaccca cgttcgcgaa cgtcttgagc ttttcaaaac
    15781 ggggggcggg tacgtgttca ctcaggtgca caacatccag ccgaacgtgc ctccggagaa
    15841 catcgttgca atgttggacg ccgcatacga atacggcgtg ttctgaagat tacgttttgt
    15901 gctctttttt ccgtgcggcg gggaaaagta cgttgttgag aataagtcgg taccccggtg
    15961 agttccggtg gaaattgagg tccgttggcg gatctcccac cagatgctgg taatcctcgg
    16021 ggtcatgccc gccaaggaac gtgaacgtac cggtcccgac ctgtccgtga acgtacttca
    16081 cctcatcttc cccttccgat tcgcccagta caatcactga tggctttatg aacctgcgat
    16141 gaaatcctac ggtctggccg aggaacccgc gaacgtggtc gacatggttc tgtgtcagca
    16201 tcgcctcgac ggggctcagt ttggcggaga agtcgaaaag cgtgaagaaa tcggcctccg
    16261 gcccgcgggg gttcgcagcg cgatcatcgt acgtatcgat gtccgagaac tcgtacaccc
    16321 acgggttctc ttccagccgg aaattctcga acgccatcgt tttggagaaa tcgagccggt
    16381 tgttgtagtc cggcgttacc ccgtccccgt cgcagatggc ggggacaatg tcaacaccct
    16441 ctgcagccag ggcaatgtca attgtgtcgg tagctgagca catggcgaac aggaacccgc
    16501 cgtttgccac gtactcccgg atcgcgcgtg cgaccgcctt cttgagctcg cttaccttcg
    16561 caaaaccgag ttcccgtgcg gttcgctcca attcggcttt ctgctgaaga aaccagggtg
    16621 cggtctggta gctgatgtag aaccgcccga attgccccgt gaaatcctcg tggtgcaggt
    16681 ggagccagtc atagtttctc aactcgcccg cgagcacctc gcggtcccag agtgtggtgt
    16741 attcgatctc agcataggtg agggcgagcg ttaccgcgtc atcccatggc tgcttgttgg
    16801 gaggggtata tacggcaatg cgcggcgctt tgtcgagtcg tatcacctcc atgttttcac
    16861 gttcgatagt gccgtagatt tctgcaacgt ccgcgtcagc aatccgttcg gacaggacgc
    16921 cgcggagctg ggattccgcg cggattacat cgctgtaatc cagcagaaag gagccgcccc
    16981 ggtaattgag cagccattcc gcggaaagcc cacgctgcag agaccagaag acaaccccgt
    17041 acgctttgag atggtcagtc tgtgtccgat ccatgggtat caggagctta tctgcacgcg
    17101 acacctctgc gccgcagaag agaagcatcg cgaaagcaat ccagcggtac cgccgcaaca
    17161 ggttcatagg gcccccggtt gtgcgcgaag ctccctcaat ctccggcgcg cctgattgcc
    17221 gtacacgctt ccgggatacg tcatcagcag cgtttccagc gctctggtct cctcgccgct
    17281 atcctgcaac cttgtgcgct ggagctccgc gtaccgccag agagcctctt ctccagccga
    17341 agatgagtcc agccgcgatg cggctgcttc cagagtggtt gccgcgaggg agtagtgtcc
    17401 ttccctgatc tgcacctcgg ctacgagcaa acgcgcccag ccttcgagtg gtgtgccgaa
    17461 aaggcgcggg gccagtaaga gaactgagtc gcggtggcct gtggcgaaga ggaactcgag
    17521 gtgcccgtac cgaacccagc taactgaatc atcggcggcc tgttcgagga tctccgccag
    17581 cctgaggcaa tcgttcgttt catcaccggg cgccgcgttt cccaccatca gttcacaacg
    17641 agccctcagt ccgggggcat cgccacgaaa catcgcagcc tgtgccaggc gcaggtgcgc
    17701 tttcagggac tctggttctt tcatcgctga atccgcgagc atctgccaga aggattgcgc
    17761 ttcctccaga tgccccgcac gcaaggcaca ttcgccccgt ccgagtatcc cctgacgtct
    17821 gtgtgccggc gtacggcctc tcgttgcggc ccagtcaaac tgctgttctg cctgttccac
    17881 ggagtcaaga gcgagaaaaa tccttccgag cgcgatacgg gcgtcgtcat ggtacgggct
    17941 gaacgggacg gagatgatcc acgaaagcag gttccgggcg cggtcctgaa aacccatttc
    18001 gtccagtatt cccgccgcag tgtacgcata tcgctgcgca gatggtgact ccgggtagtc
    18061 gcttgccagg cgctccatca ggaatgttgc caccggggtt tgcccgcggg cctccaactc
    18121 atcgatcatg gtcgcaaggg tggcgccggg attccgggtt tccttcgcgc tgcggaggaa
    18181 caggcgtacc gcgtcctcct gttgcgctcc aagtactgcc atttccgccg cgaaaagcgc
    18241 cctggcggca tcccgcggcg tgctgtccga gagagcgatc gccgcgcgcg taacagtctg
    18301 catcatggcc ggagtgaggc tggctccctg aatgatttcg cgcgcctccc gtacgcggtc
    18361 gacatcaccc gccacgaaac cagatgccgc ttccgccgtc gcatgcgcgt cgccaagcgc
    18421 gagtgcgatg tcgagcagac ggcggttcac tacgcgctgt ccatctccct gtgcgttgca
    18481 ggcacgcaac caggtctgcg cgcgacgcag ttgtccccgc gaaacaagcc tgtccgctac
    18541 accgatgtgg aatgcgatcg gatcggagga agtgcgggaa ccgatgcgcc acaccgagtc
    18601 cgcctgaacg gtatacccgg tccggtcgaa cacgtcaccg aactccaccc atagcactgc
    18661 gtcttccggt gtgtttgcag tggcttcacc gagccacgcc acggcctcat caaatctgcc
    18721 gttttccgtc agaagccttg ttcctcgcaa cacggcatcg gaccgggtgg gcgctgcctg
    18781 ccatacccgc cggtaatacg ctgcggcatc cagcgggcgg ttcgcatgtt caagagcctg
    18841 cgcctgaacg aaccagccga ttatctcgcg cggtggtgta accgccgcag gtgccgccgt
    18901 gtgcagcaga atcagcagga tggcgttggc cccgagcgcg cactcactcc atatacgtgt
    18961 taccacactg gttctgagtg atactgcacg tagcaagaat gttacgtcca cgatgtacga
    19021 tccactgagt gaacagggtg acggagaagg cagaagccgc atgtgccgct ttcacgacgt
    19081 gacagaaatc atcagtgatg ccctttgatg tgttgatccc agtactgcat ccaggcttcc
    19141 ctgtgggcat ttatgacgtt ccaatccacc ggcagttccg tgatctggat cgaacgcagc
    19201 catcccggtt tttcctgatc ggagatgtct ctccgcgcag gtatcctgta gaatcgctcc
    19261 gcttgatgaa gcagcgcctt cttcgtggtg acaaattcat agaatctttc ggcttcagca
    19321 cgatgtggcg ccccgttcac gatggcgatg ccttcgttca acacaagcgc cccactcctc
    19381 ggaatgacaa agcccatggg aacattttgt tcccgtttct ggaggaggat gtccgtcaga
    19441 tcccagagtg tgaccagccc ctcctggcgt ccgatcatga gcattagtgt cgccggagac
    19501 gccgcatagg ctttcgtgtt cgcgtcaagc cgtcggagcc aatcgtatcc gcggtcagga
    19561 gtgccgtcct ccgcgaagaa tcggtacacc atcgccgcaa acacgctttt cattgtgccc
    19621 gattccatgg gagagcgaat gatcaccttg ccctcccatc gtttttccag caggtcgtcc
    19681 caatcctggg gagccgtctg ttccgtaagt ctgtcggagt tgaaacctat caccttaacc
    19741 gtctggaacg tgctgtacca gagcccctcg tcgctgaacg agcccggagg ggcagcctcg
    19801 cgccaagagg gatggtaggg agcaagcaac ccttcccggg ccccctgttc aaagagcgcc
    19861 gatggccctc cccaccagag atcagcctgg ggattgtttt tttcgccgcg gagtcgttcg
    19921 atccccactt ccgctcccat cggataccac gtcacgtcca cgtgcgggtt cgcctcctcg
    19981 tacatccgtt cgtattcgct gagcatttct ctcccgtgag gcgaataaac gacgagcttg
    20041 gtccgttgct ccgaacagga gagattcagg gcggctccga gcgcgacgca cacttcgagg
    20101 acatggcggc ggaacgttga ctgagtaaga agggacatac aaagtctccg gaatggaaaa
    20161 cctgccgcgt gggccaactc aggaggctga cggcgcgtga acttgaaatg tacgcaacca
    20221 cggacaggcc cccacaggtt agccgcagac ggagatttgg ttgactcgtt tcggtcgcgg
    20281 tggtacgttt agtgtgccct gaaaaggtta ttggtggttt gcagtcgggc gggagatgat
    20341 aacggtctgg gactgggtaa tcctcgcgct ttttgcggcg cttgtagcgc aggacacaac
    20401 tgccgggcct cagttgctct tctctgaacc gcttgtcgcg ggaacggtcg cgggtcttgt
    20461 tcttggcgac ctgcggctcg ggcttctcct cggcaccgcc atccagctca tctggtgcgg
    20521 agccgttccc gcgggaaccg ccgtctttct cgacatcaac gtcggaacgg tatgcgccgt
    20581 tgcagtcgcg cactcggcgg gaagcgcccc catcgcggct gcggcaagca tgctctggct
    20641 gattcccgtc ggtcttctgg gatgcgccct gacagaggtc gggatgcgat tctgcggaaa
    20701 acttgtggcc tcaattcagc cggaatgcgt ctcggctggc gcgctggttc tgcggcatgg
    20761 tgcgggctgg ctgttcgcgg gggcgcgtgg ttcggtgacg tttctggccg gcgccggggc
    20821 aggcgtggcg gtggcaccgg tcctggttcg gtttctgggc gccgcggttg atcccggcgt
    20881 cctctgggca ggcctgttcg gtgccggtgc gggttctgca attgcggcga cgtggcgctt
    20941 cagcaaaggc aaggccgctt ttctgggcgc ggcaggggtt tttggtgcgt gggcgggcgg
    21001 gttgttgcct gtctcctggt gaagacacac gcgattgact tcgttgactc gggtatcgtc
    21061 gtccgaaact gcacctgtcg tgcccctgtg ggtgcgtatg ggagtcgtcc tgcgtgcgtt
    21121 ctggattcag gcaagcctga actaccagag catgcagggc ctgggaaggc tggtatgtct
    21181 gctccctgtg atgcgctggc tgagcctgcc cggaaaggaa tttgcctcgt ttgtgcggag
    21241 gcacagcggg cttttcaaca gcaacccgtt cgtcgcaacg ctgggtctcg gcgctctagc
    21301 gcggctcgaa ttcgaccgga acgcgaatcc tgaaagcgtt cccgacggca tgatcgagcg
    21361 cttttcagag cgggtttcca ctccgctggg ctctgtcggt gacgagctgt tctgggtgac
    21421 cttgaggcca catgccgtcc ttgtggggtc ggtcggagcg ctcctgacgg gagcgtgggg
    21481 tgcctgcctc ctgctggtgg tttttggtgt ctggcaggga tactatcgct ggaattcttt
    21541 cacgtgggga tgggcggccg gtgcgagaat cgccgcggtg cttcagagcg agcgttttcg
    21601 cagacccgca aaatatgcgg gctggattgc gtccgggtgc gcgggcgctg ccgtcgcgct
    21661 ccttctcgtg aactctcccg cgggcgctct gactgccaag gcgcaggtag tcgcgttcgg
    21721 gggagcgttc gcgggcgcgt gcgcagccct gtcgcagaga aaaccggcga cgtgggctct
    21781 actggcggcg gtcgggtggg caatagtggt gtcgttcggc gccaaaatgc tccgtgggtg
    21841 accggtaatt gaaggacttt tcagagttgg agctgctgtg gcaacgcacc aggtagtaga
    21901 agccgatgtt ctggtcaagg accgggaagg tatccatctc cggaatgccg ctgcccttgt
    21961 tcgagcggcg gcgcagttcc cgaaggctga tcttatgatc agcaaggatg ggcaccaggt
    22021 gaacgggaaa agcatcatgg gagtgcttac cctggttgcc gagcacggcg cccagttgca
    22081 cttgaaggcg cggggagagg aagcggacaa actgatcgcc actctcgttg ccatgtttga
    22141 aaacggtacg gaccaatgat tcatcaaccg gtggtcattc tgctgcgtga atgccatgca
    22201 taaggcgtct gacagcccca tggacccccg gctcccgcct attgagcccg cgactctgac
    22261 tcctcacggg cactgcagaa tcgtacttac gggaatcgca ggctctcccg gcgtcgcata
    22321 cggtcccgtg taccttcatg ccggtgcgga tgtctgggtt gacaaccgtc cgattccttc
    22381 cgaggcagtg gaatcagaga tcgccagatt cattgccgcg ctggcggcgg tggtggagga
    22441 aaagcgggaa ctgaaacgcc gtgcggaacg ggaaatcggc actgcccagg gccagatctt
    22501 cgaatcccac atcatgctgc tggaagacgc agacgcaatc gcgcaaaccg tggaatgcgt
    22561 ccgcgcctcg aaaaagaacg ttgcgtactg ctactttgtg acgctgcaac gggttatcga
    22621 caccatagtc ggaaccggtt cctacgaata tctgcgtgac cgcgtggtgg atattgagga
    22681 cgttcgtgcg gcagtcctga tgaaattgcg gggcgcggaa cctcaggggc tggacaagct
    22741 tgaggacgtg tcggttgttg tggcacattc cctcacgccg tctgatacgg cgcggatgga
    22801 ggcctcgaag gttgtcggtt ttgttaccga ccgtggcggt cccacaagcc acgcttcaat
    22861 tctggcgcgt tcgatgggaa tccctgcagt cgtcggttgt cagggagcat ccgtgaccat
    22921 ctcgccgggc gaaatggcgg ttgtgaatgg tttcactggt gaggtgtacg tcgaaccgga
    22981 cgaagtcctc cgtgagcaga tcgcccagtg gaaacagcaa ctggaacagc gcgcccggag
    23041 gttgctggag atatcgcggc tcccctcgca gacgcgagat ggacgccgca tacgccttga
    23101 attgaacatt gaacttgaca gcgagattca gagaacggcg gagcttgcca gtgacggcgt
    23161 cggtctgttc agaacggagt tcctgtttct ttccagcgat gaatggccga cagaagagga
    23221 gcagtacgcg gtctaccgcc atcttgctga agcattcggc gggcgtccgg ttacgatccg
    23281 cacgatggac atcggcggtg acaagctctg cagaaaactc catgtgacgc cggaaatgaa
    23341 cccgttcctc gggtggcgcg cgatacgcat ttcgctcgcg caaccggagc tgtttggtgt
    23401 tcagttaaga gctattctga gagcttccgc acatgggaac gtccagatga tgttcccgat
    23461 gatctccagt gtcgaagagc ttgatcaggc tctcatgatc ctgggccagg cgaaggaagc
    23521 actccgccgc aacggtacgc cgttcaatga ttcgatgccg gtaggcgtga tgatcgagac
    23581 tcctgccgcc gtcatgatag gcgatgccct ggcgaaaaaa gccgcatttt tcagcatcgg
    23641 cacgaatgat ctgatccagt atgcccttgc cgtagaccgg gacaatcaac gcgtcgcgaa
    23701 gctcttcgat atgttccatc cggctgtgtt gcggttgatc cacgcggcag tgaaagcggg
    23761 gcaccttgca cgcatacctg tcgaagtgtg cggcgaaatg gctcacgtgc cactgggtgc
    23821 gttgttgctg gtgggtctgg gagttgatgc gttgagcatg gcccccggaa gtattccgga
    23881 aattcgtcgc atcatccgcg ggattacgtt tgcggaggtg aaagaggttg cgcgcgcagc
    23941 actcgattcg gtttccggtg aagaggtacg gcggaacctg gaacgtgctg ctgccgattt
    24001 tggtttggat ataggtgtgg agtcgtgagt ttcttttcga acaggagaca tgatgcccag
    24061 gaacatgctt ttttcttcgg aatccgtggc tgaagggcat ccggacaagg tggcagatca
    24121 gatttccgac gccattcttg acgcgatgct ggtccaggac tcgaaatcga gagtggcgtg
    24181 cgagaccctt gtcactaccg gtctggcgct ggtcgccggg gagatcacca cgaaggcata
    24241 cgtggaggtt cccgatcttg tgcgctccgt gattcgcgat atcgggtata cggaaatggg
    24301 gtacgggttc gacgcggacg gctgtgctgt tctgacggcg attcaccagc aatccccgga
    24361 tattgcccag ggagttgacg aaggtgaagg gaaggaacag ggtgcgggcg atcaggggat
    24421 gatgttcgga tacgctaccg atgaaactgc ggaactcatg ccgatgccga tcatgctggc
    24481 ccatcgcctc gtgcttcggc tcgcagaggt gcggcacagc ggaatgttgc cttacctccg
    24541 gccagacggg aaggcgcagg tttccgtgcg gtacgaaaac gacaggccgg tgaaagtcga
    24601 aaccgtgatt gtctccactc agcataacga acacgtctcc cagcaacaga ttcgggaaga
    24661 cgtgatctcg catgtggtca agccggtcgt gggcgaggaa ttgctgggag cgaatacggt
    24721 gtaccacatc aatcccaccg gccgatttgt ggtcggcggc ccccatggag atacgggtct
    24781 gaccggtcgg aaaatcatcg tggatacgta cgggggattc agccgacacg gtggcggtgc
    24841 gttcagcggc aaggacccga gcaaggtgga tcgaagcgct gcgtatgcgg ctcgttgggc
    24901 ggcgaaaaat gtcgtggccg caggtctggc aaagcgctgc gagattcagg ttgcgtacgc
    24961 gatcggcgtc gccaagcccg tgagcgtgct cgtggaaacg ttcggcaccg ctcagataga
    25021 cgaatcgaga atcgaaaaag cagtgcgcga ggtgttccct ctgacaccca agtggatcat
    25081 ttcggagctg gatctcctgc gcccgcttta ccgcaaaacg gcttccggag ggcacttcgg
    25141 gcggaacgag ttcccatggg aacaaaccaa ccgcatcgcg gagctcaaaa ctgccgctgg
    25201 cgtctgatgg ttctggtcat tcgagaggaa atcacgggca ccggtgcacc tcgcgtcggt
    25261 gccctttgat ttggttgcaa tgacggcgtt gcccccgcgt aaggtttgag ggattcttca
    25321 tctgttcgca cttcaggaat cctgcccagt gaaagacaac ggttctgcag cgcgaaatac
    25381 tggcaagatt cggccgctca gatcctggaa aaagacacat gcgctccctc gtgattggag
    25441 tggacctggg aacttccggc gtccgggcgg ttgcagttga ccggtctgga acggtggttg
    25501 gtagcgcggc gcgcgggtat caggtccacg caccataccc cggatacgcg gaacaggacc
    25561 cggaggaatg gtggcgctcg acgtgtgaag cgctgcgtga atgcgtcgcc gtggccgggc
    25621 aaagcactat cgagggaatc gggttctccg ggcagatgca cggaacggtt ctcatcgacc
    25681 cgaagggtag cgtggtacgg cccgcgatca tctggccgga tcagcggtcg ggagaaagct
    25741 gccggcgttt cgttgccagg gtcggtgccg actggcttgc cgcccgcacg cagaactcgt
    25801 tggccaccgg ttttcagctt gcgagtctgc tctggttgtc tgagaacgac ccttcttcgc
    25861 ttgcgcgcgc gttccgtgtg ctcctcccga aagacttcat ccgaggccgg atgtccggcg
    25921 tgtgggcaac agaaccctcc gacgcgtgca gtacgctcgc gttcgactcc gggcggtgcg
    25981 cgtgggccga cgacgtgatt gctgctgcgg gcataaggcg tgacctgttt cctgaatgtc
    26041 gaagttcgtg cgactctgcc gggtgcctga gagaggcagc tgcggcggaa accggtctcc
    26101 ctgccgggat tcgtttgttc atgggtggcg gggatacacc ctcaacggcg ctcggttcag
    26161 gagtgctgtc gaagggccag tgcatggtta cgatcggatc cggtggtcag cttgttgcac
    26221 cactggatga accgcggtac gatacggctc tgcgcacaaa cacgttctgc cacgtggttc
    26281 cgcgcacgtg gtacgccatg ggcgccatcc tctcggccgg gttatccctc aaatggcttc
    26341 gtgaaaaaat actggggccc gatgcgccgg atttcgccca ccttgccgcc tctgcggagc
    26401 aggttcctcc gggtgctgag gggctcatat tccttcccta ccttaccggt gagcgaaccc
    26461 cgcatatgaa ccccgatgcg tgtggcgtct tcttcggcct gactccccgt catacggctg
    26521 cgcacatggt gcgcgcagtg atggagggtg tggcaatggc gctcgcagac ggactcgata
    26581 tcatccggtc gctccaagta accccggaat cggtgttcgc agcaggaggc ggcgcaaaaa
    26641 gccagctttg gcggcagatc ctggccgacg tgttcgactg cccgatcgct gtgagcgagg
    26701 aacgggaacg cgccgcaatc ggtgcggcga tggttgcggc aacgggagtc gggtggttcg
    26761 agaacctgga gaaggcttcc gacgcctggc gagcaccggt gaaagacgtt gcgacaccac
    26821 aggccgggca ggttgagcac taccgtgaac tgcgcgcgtt gttccgccag gtgtacagat
    26881 cgctggagga atgcatggac gcgcgcgcgg aatccttgcg caatcgacag ggcaatccag
    26941 acccgttcta cgggagttcg gaatgaaaac gccactgaaa gtgaccattt caccctcgca
    27001 tccccttctc atcctcatgt ctcctggagg cccttccgcc gaaatggctg ccgaaggatt
    27061 ccgggacgag gaggcagtca tggtgcggtg ctgggagctc cttgatgacg agataagacc
    27121 ctgtacgacc gttcatttcg gcgcgacacg cggagacaac ttcgctcacg ctgaccgcct
    27181 ccttcgtgcg gctcagacgg cgggcatccc ggttacattg cagactcaga cggacaatgc
    27241 caatatccag gatgcgatgc cgccggaaag ggtcaggcgc tttctggata cctacccctg
    27301 catcgtcggc ctgcagattg atgaagcaag ccagcgcacg tttgtgaacc acgggggcgg
    27361 cccggagtac acgatgggcc gaaacgcaag gtacgcgcgt gatatcatcc gtcttgccgc
    27421 ggaatatggg tgtttcatga gctggcaact catgcgggat aactgggccg ccattgggtg
    27481 ttccgccgat aacgaagcgc tgtatgacgc gatctgcgag tattcggaat atgtgattcc
    27541 tatgcacgaa atgaactgcg aattctccaa gttcatcaac catctttcgt gcatggggct
    27601 gtggctcgcc ggggcaacac aacagtgggg cattgaggcc cagtcgtggt attggtacga
    27661 ctgtgggtac aacaaacccg gaacgtgcga accgggcaca ctggaaatgc cgggcgaatt
    27721 gtacgccatc atgtttttgc tgggagtcag cgcaggggca tccgtgtttt ccatagagcc
    27781 cccgactgac aactggccgg ggccgggcca ctggcggttc acagagtgga tcgcgcctgt
    27841 gttccaacgc ctcctgcgcg agcacctgat cccttcagtt gaggagattc tcgcatccac
    27901 gccgctggcg taccacctgc cacggtgcga acgtcctgtt gattaccaca aggtgctggc
    27961 cgatctggat tttgaccacg gtgaaggcag gctcatacga gtaacgtatg gcgttttcga
    28021 tcgcgcgcgg gacgcggagt tcattccgaa caacccgcgg tacggatgga tccccgtcct
    28081 gcctgcgaag accccggagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    28201 aggccatggt tggttggaaa tctgccgtgg agtcagaaga aatcccgcgc atcatggcct
    28261 tttacgccga agattaccgt gaaccggatg gaaggacgcg ggaatccgtg gaagtcgcgt
    28321 tcaggaacct ttttcgcaga tatgtcatgg atcggttcga accctttctc caggaatggg
    28381 gtgcagtgcc cggatggcag tttccggcac tccgcctgtt gatccgcgag tggggcgagg
    28441 catcgtcgca aacggtcgag gtatcggcgg ttgcgcatct ctgggcgggg ggaggccctg
    28501 aacttgaacc gagtgacatg attatcattc cgttcggacg gccgtcgctg accacgttcg
    28561 tgtggacgaa cgcggggcgc ggctggaagc tggcgaaaac ggcccccgcg tttcttcaag
    28621 tggaggacac cgcggttttc cgatttcgtt atcaggggtg gtgaacctgc cctgcaagca
    28681 atgcatcagg acacgaacag gattgctcac tcgaacccga ctgctcgtcg aaatctggac
    28741 caataccatg gagaatgcat gaaaacgccg ttgaaaagga ctatctgccc ttcccaccct
    28801 ctgctcatcc tcatggcgcc tgccgggcag ggcggccacc ttgcaacccg ggggtacacg
    28861 ggtgaagctc gattcatggt ggaatgctgg caccgtcttc ccgacgacat caaaccgtat
    28921 gtgtcgattc agatcgaggg gatttgcgac gaccatttcc gtcggaacga agtgctcctc
    28981 ccgatcgcgc aggcggaagg ggtgccgatc accgtgcagg tgcagaccaa caattccgac
    29041 ctcaatgaca ccgttccgat ggacacagtc cggcggtacg tcgacacgta ttcctgcatt
    29101 gacggcctgc aaatcgccga ggccagccaa cgcacgttcg ttgctcacgg cggtgggcct
    29161 gaatactcca tgggccggaa tgcccgttac gcgcgggata tcatacgctt gtgcggggaa
    29221 tacggccttt tcatgagctg gcagttgatg tccgaaaact tcgcggccat aggctgctcg
    29281 gcggataacg aagcgttgtt cgataccgtg tgcgagtatg gtgagtacgt gatcccaatg
    29341 cacgagatga attgcgagtt cgcgaaatac atcgatcatc tgtcagcgtt cgggctctgg
    29401 ttctccggtg ctacggcgaa ctggggcgtg gaagcacagt cgtggtactg gtctgatgct
    29461 ggactgagta ctccggggag tttcgagccg gggtcgctcg agatgcccgg ggagatgtac
    29521 gccatcatgt tcatgctggg cgccgtgggc ggggcgaccg cgtactcggt ggaaccgtcc
    29581 tgggatatct ggcccgggcc cggcgagtgg cggttcaggg actgggtagt tcctacgttc
    29641 cggcgcttgg tcagcgagag gctcatcccc gaccgccaca acgtcgcgaa ggtgacgccg
    29701 gtcgcgtacc acctcccgcg ttgcgaacgg ccgctggaat tccacgcagt ctcggacgac
    29761 ctggatttcg accacggggc aggccgcctc atccggggga cgttcggagt gtacgaccgt
    29821 gcgcgggacc cggagctgat tcccaacgat ccgcgtttcg ggtggttccc cgttctgccc
    29881 gtgaaaacgg acaggtcggt gctgacgcaa ttcaaacgtg ttctgcggcc aggggacgtc
    29941 aattcgccag agcatgcgcg ggaactgatg gggaagtact accccccggt cgaccgcgga
    30001 accgcatgga gccagatcgt tggtgacctc gtgttcgcgg caaataccca cgaaaactgg
    30061 ttcgttccgg aagaagtcag gctgcaggtc ccacgccgtc ctgcgaatgt gagaatcgaa
    30121 tccactggga cggcacaact tcgcctgcgc tgggagaagg ccgaaggcga caacgcatac
    30181 cgcgtctggc ggatccgtga cggtgtggaa acatgcctga ccccgaactc gatcacagag
    30241 accgagtttc tattgccgcc tgtggaacaa gccgaccagt tcgcggtctc cgcgatcact
    30301 tcagccacgg aagaattcgc gcagaccctc caccttcatc agtttctggt tttgaacagg
    30361 gccgagagcc gccggagtga atgggtaaat gccgcgggcg acaggatgga acgcttccgt
    30421 tttgcggaat ccgtcccgcc agtatctgag cacgttctgg aagcggaacg gagatgcgcc
    30481 ggttgcttgc ccgttcagga cctcacctct cctccggtta cccccgacga ccccttcgcg
    30541 tctgtcaaac tggcgatcat ggaccacatg gcccaatgga agtcattcgt cgaagctgaa
    30601 gacctcgacg gaatcatgag tatgtacgca gccgattact cagaaccgga cggccggggg
    30661 aaggactggg tggaagccgc cttccggctg gttcttcgcc gctacatgga gagccagttt
    30721 gccaggcttg tgaaagagtg gggtgccctg ccagggtggc gaaatcccgc gttccggctt
    30781 cttgtcaggg aatggaggtc ggtgagcaat gatgcggcgg aggtgctcgt cgtggctcac
    30841 atgtgggcag gcggggggcc ggagatggaa ccgagcgaca tgttcaacca cccgttcggc
    30901 cgcccgcaca cgacgaccat gaggtggaaa ctgatctccg gagaatggaa aataacgtcg
    30961 accgacccag catttcttcg tatcgaggac acagcgatct ttcgcttccg ttaccagggg
    31021 tggtaggagt attgttgcca gccctccccg gttgacccgg ggagggctcg gtctttgcgg
    31081 gcggcttcta ccatttcctc aggaaatcca gcgccttctt cacgtctgcg gcagggtcat
    31141 ccccgacttc ccgctcgatg gtgtagaacc cgcgatagcc ctgagctgtc atgagatcga
    31201 tatactcttc ccatggcact gcaccctcgc ccagaggaac ttcttttccc ctcccatcct
    31261 cgcggatgcc atcctttgcg tgcgtgtgaa cgatgtaagg tcccagcgct ctgacacctt
    31321 ccagaacgtc gaagcccatc atcgcgaggt tggccgggtc gaagtttgcc ttcacagcct
    31381 gcgttttcag gctttttatg aagtccgcaa gaacgggacc cggttccggg ccagtttccg
    31441 ttgcgaacat tatgcccctg ttatccgcat atgccccgac attgttcaat gcgtctgcca
    31501 tgatctgacg gcttctggct ttcggatcag catccacaac accgatgtga gtggtgatga
    31561 tcggcgtttt caggtccacc gcgaggtcta tcactttctt tgtgtctgtt atgagttcgt
    31621 ccaggccttc ttccttggtg tacggcttgc cgaaatcgcc acagagagca ctgatgacaa
    31681 ggccctggtc cgcaacgaac ttgaggaact cctgtctgcc agtctttgag aggttgtcag
    31741 ggtgcatctc tccgcccgtg acatagatct ggaacccgtc ggcaccgatt tctgccgctt
    31801 tcttgatgcc gtttcgcacg ccgagccgga atgactcgat catgacaccc actttgaggt
    31861 tgcccatcgc gtttctcctg aatttgtgga gtgagacact gtttcgttga tggtcgggag
    31921 ggctattgag gcagcctccc gcccatgttg tgcggtgatg cgtacaagtg atctgtccgg
    31981 atgcggaatg cttcagtcaa tccccatgcc tcaagggctt ccgccacgtc catgattcga
    32041 tcgccaaacc gctccatctg acgctgcaat tcctggtgga gactggctct tgtttcggct
    32101 gccaatggat cgccggcgag gttttcgagt tggtaagggt ccctttcgtc atcgtagagg
    32161 agccattctc cctgcgtatc acaggcatac gtgtagcgct cggttttgat cccgcgccac
    32221 ggcttgattc cgtaatgatt cgcctgatcc gtcgtgatga gatcggcgag atagagaacc
    32281 cggccttctt tcattgcgcc actccgtaca caggccgaaa ggtcttcgcc gtgcatggag
    32341 ggtggaacct gcaggtcgag gcaccccagc agcgtcggaa caaagtccag cagactgaaa
    32401 agggtcttgt tcacgcgtcc ttccggaaca gcccccggcc ggtagaaaat caacggcact
    32461 acgaccgact ccgcccacgg acgttgtttg tgcgtgtgcc cctgtgatcc cagcatggat
    32521 ccgtggtccg atgtgaacac cacgagcgtg tccttttcga gattgtttgc acggaggtag
    32581 tcgagcaggc gctgcgtttc cgcgtcaagg gctgaaatgt gagcgtagta ccctgcgaga
    32641 tgccggcggt tctccggtgt gtccgggcaa tttggcggaa ggaccagcgc ggaaggatcg
    32701 tactgggttt cccttcccgg cggaagcggt tcgtagggtg aatggggtgg cccccatgaa
    32761 aggaaaagac aaaacgggtg ttcgttgtct tgttctctgt gccgatccat gaacgccatc
    32821 gcgaggtctg tctgcacgga tggctcatac ccctccatca caaccggttc cggggtatcc
    32881 aggtaataac gcgggttcat gtaatcatgg tggcaattcc acacagccca gaatgcgtca
    32941 aatcccagtc tctccgggcc tggtggagtg aatttgtacc gtggaatgcc tcccagatgc
    33001 catttcccta tgtagccgca ccggtaaccg tgcgagttca ggatgtgcgc gatgctcgtc
    33061 acatcggttc gaattgcgat gtcgttcact accgcaccgt gggccgttgg ccagaggccg
    33121 gtcaacattg tccctctggc gggcgtgcac acaggcgtat tggaatacgc gcgcgggaaa
    33181 cggcatcctc gcgaagccat ggagtcgaga cacggtgtgg atacgttcgg gtcacctgcg
    33241 caacccatcg cctgtccccg catttcatcg ccgaatacca caattacatt ggtggccacc
    33301 gtcgctcgcc tccatcatct caaacgcgcg ggaaatcctt ctttatccag cgtgcgtatc
    33361 gccgcatctg ccgtctggat gtctttgaac gccatgcgga atgtaccgcc ctcgccttcg
    33421 cggattttca gcagctcgat gtccatgatg ttgatctgtt cgcgtgcaag agccgccgtc
    33481 atgcgggcaa gctgccccgg ccgatcctcg cagcgcacaa gcacatcgca cagaggagaa
    33541 agaaaccctt tcgtatctct ggggattgag ctccgtatct cattggctgc gcgaaaatgc
    33601 ggttccatat gctcgatatc tgccagcacc tgctcgagtt ggccggcgaa gccgcggagc
    33661 gcctttcgta tttctgaggc atttgtcgcc agaatgtcct gccagacgct gtacggactt
    33721 gacgcaattc tggtcatgtc acggaatccg cccgccgcca tttgaaggtg cgccggctcg
    33781 attgcgttca gtgtgccgac gtattccaca agggcgagcg ctatcatctg cggaaggtgg
    33841 ctcacagcgg ctgccactcg gtcgtgagcg gaagcggaca ggcagaatac tcgtgcaccg
    33901 atgctcgcga tgcgctccgt gaaagacgcg cgcacactct ccggcacgga cccggagggg
    33961 gtgatcacat acagcgcgtt ctggaacaga aacgggtccg ctgcaccgac tccgtttcgt
    34021 tccgagcccg ccatggggtg gcccccgata aaaaccgagt gctgcggcat gcatttctcg
    34081 gcccgttcca cgatctcttt tttcgtgctt ccgacgtcag aaacaacggt gccgggagtg
    34141 atgacgtggg ccacctccgg cagcagttcg atgatccgag aaacgggagt gcaaagaaac
    34201 acggccttcg tttcgcgcaa tgcaagggcg aggtcgttat attcccatcc ctcgtctatt
    34261 gcgcccagac cgagtgcctc cttgatcgtt tttcggctgc tgattccgac cacacggccg
    34321 gtcgcgccaa attccttcag cgcaagggcg agcgatcctc caatcaggcc gaccccgacg
    34381 attgcaactg atgagaaatc cgcccctgga cgttcatcca tcgtcaggcg actccccggt
    34441 gtgcggcacg cgcggtcgcg atttcaacgc ggcgcaggag tgatactgcc tcctcgggtg
    34501 tccccacgcc cgtcgtgatg aaaagcccct tcgggcctac ttcgtcgata aggggctcga
    34561 cgtcttctgc cgggacgtac acatgaacac ctttgcccat cgcgaggatc cgtttgagaa
    34621 ggtccggcca gtaacgcgcg gagggattgc cgtccccggg aacccattgg atcgccttga
    34681 ggtgcggcaa ctgcgacagt gcatcaagat ggcagatcgc gttcggaccg tccagatgat
    34741 agatgggatg gtccaatctg cccacgaacc gttccatctc cggcatgacc atccatcgga
    34801 aggtctgcgg ggagatcatg cacgagaaat cgcactgcag tgggtaagtc cgtttcggtg
    34861 accatgcgcc cagccatccg catgagcctt cctgtcgcgt tcgggtaatt gcaagcaatt
    34921 cctcgtacca ctgaaaccag agggaggtga tctgcgccag cgcccactca acgtcatacc
    34981 ggttctccag tgtatctaga aggagttgct gtggcccccg cagatgcgcg agaatatcgg
    35041 tcgaattccc gaggtccgtg atgcttacga atgagattcc ttccagctct tccgccgctt
    35101 gccgcgtgat cgcaagggtc cgccgccacc acaggttctg agaatcgaag cagaacggaa
    35161 gccacgtatc ccaggatgtg attaccgggc tttcctgcca tgcggtagtc tccgcaaagg
    35221 caagctcgca ccccagatag gcgcttccta cggagggccc cagattgacg aacatcagag
    35281 ggaaggattc gccaccgtag taggtggtct cggcgtgcgc cttctggcgg gcaatcaggt
    35341 aatcggcatt tgtccagcgt tccaccatgc tcgcgggagt ggggatctcc ttgcgtggcc
    35401 ggccgttatg tgccgtaaca gctaccaccg cacgatcgat gatttcgttg ttccaccacg
    35461 cctcgattcg ggtgcgtgcc ttttcccagt cgtctttgca gcggaattcc acctgtgtcc
    35521 ctccttgaat ttcacgccgc ccgaaagatg atgcggtagc ccgcgtcgcg ctcagcgaat
    35581 tgttcgaccg aggaccgggg taatgagcct caactgcccc atcaattcgt cgaactgatc
    35641 cagattcaat gtttgcactc catccgaaaa cgcgtggtcc gggtcgacgt gggcctccac
    35701 aagcagtccg tccgcccctg cagcaacggc agcacgtgcc atcgggatta ccttgtccct
    35761 tcggcccgtt ccatggcttg gatcggcgat gattggcagg tgactcagct tcttgatgac
    35821 gggtatcgcc gcaatgtcga gcgtattgcg cgtatacgtc tcaaacgtcc gtatcccgcg
    35881 ttcgcagagc acgatattgt ggttgccccc ggccatgatg tattcggcag acatgagcca
    35941 ctcttcatag gtcgcagaaa gtccccgttt cagcatgatg ggaatgcgcg ttttaccgag
    36001 ttcgcgcaga aaggtgaaat tctgcatgtt gcgggcgccg acctgaagca tatctgcgta
    36061 tgcgacaacc gaatcgattt gcgtgcgatc catggcttcc gtcgctactt tcagcccaaa
    36121 ttcgtcggca acagtgcgga ggatctccag acctttttct cccagtccag agaaactgta
    36181 cggggatgtg cgcggtttga acgcaccacc ccgaagcatg cgggccccgg ccgctgccac
    36241 acgttccgcc gtacgtctgg tctggtccag agactcaacg gaacacgggc cggcgatcac
    36301 gactatctct ttgccgccta tcggcacgtc cccgacgtag actacgctgt cctcccgctt
    36361 gaacgtgcgg ctggcaagtt tgtagggctc actgattcgt accacttcgg cgacaccagg
    36421 catgacttcg aatgcctgca gatcgagcat atgtgtttcg ccgatcgcac cgatgacggt
    36481 tcgcgacgtg ccggtcgatc gatgcacgtc cagtttgtgt tcctgcaaac gcgcaataac
    36541 ggccgcaatt tgctcttcag tcgcccattg ttccatcgcc acaaccatgg cgcattacct
    36601 cctgtatggt cagatgtgtt cagaatccgc tcggtgaatc aagttgcatt cccttgcagg
    36661 ctttcttcga gtgcgctgta aatgcgctcc aaccgccggt tctcatcgat gattcgttca
    36721 aacaagcgtt tgacggcatc cgccgtcaac ggcccctcgt tcagatccac aacctgttgc
    36781 aggacaacac tttcacggcg tggatcgtaa atcggcaggt tcatccgacg cttcagcttg
    36841 ccgatgttca gcgagcactt cgcgcgctcg ttgagcaaca cgacaagctg gcggtcgata
    36901 tcgtcgattc tgtcgcgcca gtcggcaata tccatgttca tcccccgatg aattggccgg
    36961 tgagtgtcct tacaaattcg gttacctggc ggaccatttc gtcggtgtct ccaccttcac
    37021 cgatgcgccg aactatcgca ctgcccacca ccacgccgtc ggcaaactgg ccgatctcgg
    37081 aaacctgttc gcgggaggag ataccaaatc ctacggccac gggaagttgc gtcaaccggc
    37141 ggacgcgagc cacgttctct cggatgccgg aggccagacc attccgttca ccggtgacgc
    37201 cgcttcgcga aacgcagtag acaaacccgt ttgccagctc gaggatatgc ggcaaacggc
    37261 ggtcgtccgt ggtcggagcg acgaggaaaa tcgttcccag tcccgccccg tcaaacgccg
    37321 gtttgaactc ctgcgcctct tccaccggaa catcgacgag cagcacgcca tctgctcccg
    37381 cagaaaccgc gtctacggcg aacttctcta caccgtacgc gaacacgggg ttgtagtacg
    37441 tgaagaggac gactggcaac ccgaagccct cgccacgaag cgcttccgtt gtgcgcaaaa
    37501 cgtggcgaag ggttacaccc ttcgcaagag cccgcaaagc ggcttcctgg ttcgcggtgc
    37561 cgtccgccat cgggtccgtg aaaggcacgc ccagttccag gatatccacg cccgccccgg
    37621 caaattcgac ggcaagttgt ctcgtcctgt ccagcgtcgg atcaccgcac gtgatatacg
    37681 cgatgaacgc gggacgatgg tgctctttgg ccgacgcaaa ggcacgatga atacgtgaag
    37741 atccgcagtg gtcgtgattg tattcgttca ttccgccagc atctccccca ccgcacccac
    37801 gatgatgtcc tccgcgtcgg cagcgagata cgagggcatg ttttcgtaga agtaccagcc
    37861 gtcaacttcg tacccaccgt cggcatggat ggctttcgaa gggacatacc cgatcacccg
    37921 gtttgtgtaa ccgagaacga accgcggtcc gtccccgatc agggacttga tccgcaagcc
    37981 gatttccaca cacacttcgc cggcgatggc gaccagtgaa agggcgtttc cgatttgcag
    38041 agtctgcaca tgaacggagc aggttgtggg aaggggcaac tcccgggtgt acgcgtccag
    38101 catgcgccac gcccaccgcc tgcggaacgg cgaaccgtct tcggtcgcgc gagcaagctc
    38161 cgcgcgagac ggatgcggca tcagcggaag ttccagaatt cgagcgccag tcttcaggtt
    38221 cccctcgatc gccgcacccg gttctgccac cacgttcagg actgcctccg ccagttcctc
    38281 accgaatttc tgtacgacct caagaggccc acttttgaag ttccccgtct catccacgtt
    38341 attggggcgc acgtttccac tgcacccttg tacgaaaagg gcagtcgctc ccggcaaagc
    38401 gctctcgatt ccgatcttcg cgaagcccgg gtagtctccg ccaagaaggt acccgcccat
    38461 cgtggtggga tggcaacagt aaccgaacaa aatcgccgcg atctgcccgc tcgttcttgt
    38521 acacaggatg tcaacgtctc ggtccaccgc tccgtcgcga ttcgggcgca tctgcacacc
    38581 cgaaggcgtt ttcacccgcc ggttgatgtt gaacccgcac cggccgcggt ggtacgtcaa
    38641 cgtgcacggt gccatctccg ccactgcttc gcgaacactc tccaccagct tctggcggat
    38701 ctgatcaaga tactcctcat cccgccaccc gtacatgtcc cgatcaccgc gccgggtgac
    38761 gggcccgcaa tgcgtgtggg aagcagtgat gaacagcctg tcggggggga ttcccgggtt
    38821 tcgcgccagt tccacctcca cgggtccgat tacatcatca tcgaaataca ggatatccga
    38881 ggtgacgata accaccgtgg cgttcccgtc acccaaagcc agtgctttca ggttaagatc
    38941 ctgatacgca ccttcgctgt ccttgtctcg ggatgcgtac cctgccatcc ggaccgggcg
    39001 ctcgggattg atgcaacgag ttgcaacccc gcacttcatg gtgttcccat cctttcgatt
    39061 gcctcgttgc gatatctgcc tgctttgttg gccggacatc acggactgcc gtacattttg
    39121 tgaagaccaa cccacgaaat gcccccttcc cgtgagagga catgtatggc cggcgaccat
    39181 atggaccgct tcttcggtaa cgataacgac cgccccgacg aatgggacgg cgaagacaac
    39241 gatttttctg acgaggacgc ggagtttcca caaagcggtc tggaaatcgc gatttcaccc
    39301 tttctggggt acgaagtcga gctgcagggc actttcccga tccagctcca gataatccgc
    39361 ccggccaccc ccaattccgc gcgtgtactt gccggtgtat atccagattg catcgtggtg
    39421 ttcgatcagg aacactacct cgtcttgccg cagacaatgg tcatcgtttc ggttccccga
    39481 gtggaatggg aatcccgtga gcctctggag cccggcgacg attgaaccac gaaccacacc
    39541 gttcgtttcc gccggaatca ggaatccgtt atccccagcg cgtgagccag ctcacggggc
    39601 acaggaacca cacgcccttt gtccaccacg gcgtgtacca tctgaccgat acccgcggta
    39661 attcctgcag atgccgcaac aaatcggtac cgcagtgcta cgcgggtccg gccttccaac
    39721 cggctttcta cctcgattga tacgggatca tcataccgca acggggaaag aaacctgcac
    39781 gaggcttcca cgaccggata cgtctgcccc tttcggaacg attcgacgac atccatgccg
    39841 ttgtccgcaa ggtgctcact ccatgcagct tccatccagg cgaagtaatt cccgttgtac
    39901 accactccga acgcatcagt ctcctcgtaa cgtacccgga gaccagtgtt ccagtgtacc
    39961 cgggcgggtt cctcgagcga gggacgaggt atcgactcgc cagtcggtgc aaacgcctcc
    40021 gcgaaacacg cgggcagacg cgtcggcctg cccgagaaat ccgccgcgat gtggtcggta
    40081 taacccgtca cggccgggga ctcatcgttt cctgtgaaca ccgcgtacgc gaaggagcac
    40141 ctcgcccgcc ccatttccgc cattcgcgcc tcaaccgcga ccgtcgcgcc tgcaggaatc
    40201 ggtctgtgat actgcgcgcc ggcagcagtg acgaacgagc ctatccggtt tttttcgagc
    40261 tccacgtagg gcagccctgc ggcgcgtccc atttcggtac gtgccacctc aaaccaggtg
    40321 aacaacgtcc cgaagtagat gtggtcgaag gatatctctt tcagccgact ccggattaca
    40381 cacgtatgcc ttcccgtact catctccgtt ttgacctgtc gggtggcgga agaaaaaggt
    40441 cctcagtgac gtcaacggtg ggaatacccg cgtaccgccg gtcctccgca tttgccatga
    40501 tgttcagaga tctgatgtcc ggctggcgat cccacttaag caggcgtacg cctttcgcaa
    40561 aaacccggct tgcagatgcg tcaacgaaat acgtcggtcc cgaactgccc gtcgaatcgc
    40621 gccagatatc ctgtcctgcg ggccacgtcc cctgggtttc gcaatccagt ctgtacatcg
    40681 gttccgtcgt tcctaccacc ggtttcggcg ggtctcccac ccatcgcacc tcgatcgtgc
    40741 tgatgcccgt cagattcggc accagcggcg tgaagtccat gaacaagggg ctgaggggct
    40801 tccactccgg gctttgggaa atgtccggcg gtcgaacatt gcgaacagaa agagcaattt
    40861 cctcgtcgaa ggctccacac gctacggcca gaagctcgtc cagtttgttc tgcatcccca
    40921 caagttttac ggcgacggcc gaaagtcggt aggtgggact tcgcgggtcg cgcgcatcgt
    40981 ccctcgcgag gttcacggtg tcgcacaggc cgatggcagt ggttgcccca ctcttgtacg
    41041 cttcaagtat catcgaaacg acgcccgcgc ttcggctgta attcatcggg aaatcgcgag
    41101 ttacgagaag tcggtagtcg tagcactgag tgaagtcccg cgaattccga acattggccg
    41161 cttccgcata cacgagctga gccggcacat cgagcagcct ctccagattt gcggccacca
    41221 catcgccatt gacctgaggg attatcccga acctgtatcg tactccccag ctccacacgc
    41281 cgaaacgcat ggtgcgtttc aggtctgcat gaagcttctt ggccgattgg acgagcggct
    41341 tggcaagtgc cgagtcgggc gcgctgccca gcacagacgc gaggtgctcc tcggcaagcg
    41401 cccatttctg agtcacgcgg gagaactgcg ggcggccggc ggcgtacgaa cgcgcgacac
    41461 tcactccact gatgatgccg tacgcaacaa ttggcacaag cgcgacgatt ttcgctttcc
    41521 ggctgagtct ggggtgaacc cacacccctg tcaacagcac aacccagagc gcgaacagaa
    41581 cgatcaagcc tgtcatggag cacgatctcc gcggaagtca gaatgtcagg cgatgcggac
    41641 acgtcggtgt gtttcgcatc agaaaatgta agcggagaca ccggagaaac cctagccgaa
    41701 acgcttgaat tcgacccctg tctgcgcata tcattagtat tcgacctgac aaaagggaat
    41761 gcatatcctg atcaacccgt cggttgtgtg gccacttcca gaaacgaaag gatggttacc
    41821 atgtctgcgc tcaagggtat tccgatgagc atctcccccg aattgttgga ggttctcgcc
    41881 cgcatgggcc acggcgacca catcgtactc gccgatggga actttcccgg ggaaagccag
    41941 ggggctgatt acgtgatccg agtggatgga atgggtgttc ctgccctgct caaggacctc
    42001 ctgaagctga tgccggtgga tacaggcgaa agctcagcat acgtgatggc ggtgcccaaa
    42061 ggccaaccgg aacccccgat atgggcaaag taccgcgaaa tcatcaacaa agccgaaaaa
    42121 cgcgaagtga aacttgtcga actggaccgt ttcgcattct atgacttcac gaaagagaaa
    42181 gccttcgcga tagtaatgac gggcgaaaca gccctctacg cgaaccttat cctcgtgaag
    42241 ggcgtcgtcg ccccgaggaa ataggtggga gaatgcacgg aaaccaaatg ggacgagagg
    42301 aggagccaat tttgtctttc gccaaagata tgaggt
//
LOCUS       DAOSOO010000004        41311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1004_length_41311_cov_4.689475, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000004 DAOSOO010000000
VERSION     DAOSOO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 41311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41311
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1004_length_41311_cov_4.689475"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLT86_00540"
     CDS             <1..976
                     /locus_tag="PLT86_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT35136.1"
                     /translation="DGRPDIIASNSYGEVWQWLSASPDCSWVFGAGTRVRAGGKLIRI
                     FAGASGSIQGPGEARYGYGAPEVADWNGDGALDLILADVWGKHTLYCGLGADLRRTDA
                     NAGLELSEGQPLRFANPRDVVKPAWNWWNPVGGELVTQWRTRGEVLDWNGDGEVDYVT
                     IDHEGYLALAPGVRTREGRMLEPLERVFLNEAGEPLLLTPGTCGRSGRYKIALADWDG
                     DGDLDLIRGVATPSGNTMHTPSEETGCAFLFENVGNNRFAFRGEMVPDPTIQLAGHSS
                     CPQPFDFDRDGKLDLLIGAEDGHIYAFHRSYLEKDLPRVQVTRVRVTH"
     gene            complement(1012..3477)
                     /locus_tag="PLT86_00545"
     CDS             complement(1012..3477)
                     /locus_tag="PLT86_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT35137.1"
                     /translation="MDVDRFLKRIGAESGWRRQVVHVQRIPAREATYREPDVPLDAAI
                     ADVLKAQGISRLYCHQADALESARSGRGFVVVTGTASGKTLCYDLPIFERLLVEPDSC
                     ALCIYPTKALAQDQLRGVLRYLQGLGLGVKAGTYDGDTAPTTRRVLRNEGQVILTNPD
                     MLHAGILPHHARWARFFEKLRYVVLDEIHTYRGVFGSNVAGVLRRLNRICAHYGSKPL
                     YLCSSATIANPLEHAENLTQRSLTLIDRDGSPRGEKSFVLWNPPLLDDPSAGETPERR
                     SALSDAQYCMVSLIEERTPVIAFVRTRVSAELLYRFVQDVLSHRNASLARRVRAYRGG
                     YLPEERREIERQLFEGELLGVISTNALELGIDIGTLEASILIGYPGTIASTWQQAGRA
                     GRGKERSLVVLVAHNTPIDQYLMNHPQYFFERNPEHAIIDPTNPHIAIGHLLCAAQEL
                     PIAPEERVDLFGEYTPALVEILADEGHVRRVGERWHFARSGYAAAKIGLRNMDDTTYT
                     IMNETDQGPQAIGTLDEISALSQVHTHAVYLHDAETFFVDKLDVDRKIAHVRREGVDY
                     YTQSVSESSIVVRNTEREGAWRVSKLALGDVTVSLNVVMFKKIKFGSRESIGYENLDL
                     PTQTLDTTAFWLVPRAAALDECRAWGRVPAEGLKGIANVLVEIVPLFVMADTTDIGSI
                     VDSSNLGTPAIFLYDKYPGGIGFSERTYQWTEQIFTAAWELVSECPCIDGCPSCVGAP
                     PPAGGHDAAGERDIIPDKETALILLHHFLEKPPYTPKQRVAPSNTRMLPAVEEPHVPV
                     KPLPANEEAKVRSRLQWLKRKKA"
     gene            3666..4400
                     /locus_tag="PLT86_00550"
     CDS             3666..4400
                     /locus_tag="PLT86_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT35138.1"
                     /translation="MKGVILAGGTGSRLFPLTKVTNKHLLPVYDRPMIYHPIQALVNA
                     GIRDIMIVTGGENAGDFLRLLGNGSEFGLQHLNYAYQEKPGGIAQALALAEHFVGNEK
                     FVVALGDNIIQKSIRAAAQAFEHQPEGAKILLAEVDNPRSYGIARIDGNAVVEIIEKP
                     SDPPSNFAVIGIYFYTPEVFKIIKTLKPSGRGELEITDVNNEYLRRGTLTFEYLDGWW
                     ADCGESIPWLLRANNLVARDGANNAG"
     gene            4542..5621
                     /locus_tag="PLT86_00555"
     CDS             4542..5621
                     /locus_tag="PLT86_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35139.1"
                     /translation="MADKIRMGFIGCGFMGQLAHIANYARLHDECDLVAVADVRPLLA
                     KKVAARYGIRETYTDHRELLEKADVDAVAAIMYYGHHYAVVPDVIESGRHLITEKPMA
                     CSASSAREWKAAADAKLLVYMVGYMKRWDLGARWVSDMVREWKTTNEFGEFTHLRCLM
                     SGTDWTWNPYPVISSDEQPMTSLPPHEPFPARFTKAQADYFNMNINFYVHQVNLMRFL
                     LHEQITLDYCHPAGKVLIGSTESGKAVSLEMGISSIPQTWDEVYQIAFEKADIELRMP
                     APLRGQHNGEVVVRRTVNGKTYETLRPNFPVPSWSFLEQAKGFLEAVRTGRVPFDSTG
                     DAVRDLEFFETQADMMQGEGGVARK"
     gene            5654..6541
                     /locus_tag="PLT86_00560"
     CDS             5654..6541
                     /locus_tag="PLT86_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35140.1"
                     /translation="MVVELEKGELRATVVDNAAHLERAGHREGYNGLAALTSDAQQAS
                     PIVPFYAGMNLELYFDGVVTDRSTLFEPRRAPMAVEKIDQATARLHQPPVPYWKVESW
                     TEFTLREPNYIDFRYRAIPHERTFEQNWLGVFWATYVAYPEDPGIWFPTATPEGAKTM
                     RHFSPVHGDESTHRHAADSEEITVSHDHRIYMYGSYSRFRYARPFFWGFVHGMSVLFM
                     FDDNPNIRFAQSPSGGGTRCPAWDFQLVVPDYRVGGEYSLEARMVYSRLGATLDPEVE
                     YARWVEFRKNNRSDGGEGR"
     gene            6538..6888
                     /locus_tag="PLT86_00565"
     CDS             6538..6888
                     /locus_tag="PLT86_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35141.1"
                     /translation="MKDQSDGVQSWPERGERNGLTRSPIAGLDGLLCPVCGEIADSLP
                     IKVQRVIQGTEECLFVCSPDCRQMALESPFLQINMFSRDMRVWERSVQSELDIESSPV
                     AEAPETEHNGREHS"
     gene            6885..7805
                     /locus_tag="PLT86_00570"
     CDS             6885..7805
                     /locus_tag="PLT86_00570"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOT35142.1"
                     /translation="MTKAGKAMTDVELVLDAKADLGEGAIWHARRGLLYWVDINAGFL
                     HVFEPKNGKDDAYRIGEDVGTVVPRVASSERPYESVMLALRRGFAAFRFDAAKAYVVA
                     DPERDIPANRFNDGKCDPAGRFWAGTMPEGPGGVPDGGRLYCLYPDLHVEVKETGIRC
                     SNGIAWSLDHRTMYYIDTPSMEIWAYNYDLETGAIWDRRVVVRVPKNEGYPDGMTIDR
                     EGMLWVAHWDGWQVVRWNPKTGRKICSVKVPAARVTSCAFGGKNLDELYITTARTGIK
                     AEALRKQPHAGGVFRVLPGVSGLEAFEFAG"
     gene            8057..8344
                     /gene="gatC"
                     /locus_tag="PLT86_00575"
     CDS             8057..8344
                     /gene="gatC"
                     /locus_tag="PLT86_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT35143.1"
                     /translation="MPVSREDVLHVAALARLEVAPEQVESFQRELGAILEHFQKLNEL
                     DTSNVEPTSHALALVNVFREDIPQPSLTQEEALANAPQKAFGHFRVPKVID"
     gene            8392..9147
                     /locus_tag="PLT86_00580"
     CDS             8392..9147
                     /locus_tag="PLT86_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT35144.1"
                     /translation="MGVVPARFGSERWPGKVLENVSGKPLLRWAWETLSGDPEVSEVL
                     IATDDSRVETWARAQGMRVVKTSAECRNGTERTAEIARMIRADYYVNVQADQVALESR
                     VVTAMLRAAKAHPEWEIATAGTRWYRTGCLANRDRVFLAVRGCTCGRHSIAEHFFRGG
                     EKHTNTDRPVLRHLGLYGYTPSFLREYAANEPSQDEVILHLEQCRALQMGLEIGVVRV
                     RTSARSVDSAMDRDAVEGSWSNKTSKGDPREDA"
     gene            9134..10966
                     /locus_tag="PLT86_00585"
     CDS             9134..10966
                     /locus_tag="PLT86_00585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT35145.1"
                     /translation="MRTREQTFPKYIVVAGGVISGVGKGLATSSIGRILQEFGYSCTA
                     VKIDPYLNYDAGTMRPTEHGEVWVTDDGGEIDQDLGNYERFLGKELSRRNNLTTGQIY
                     KAVIEKERQGEYLGQTVQPIPHITNEIKRRVTEASAGHDIALVEVGGTIGDYENTPFF
                     FALKSLEREIGKQNIVYVLVTYLPVPTHIQEMKTKPTQHSIKLLSEQGIFPDFILCRS
                     KSPVDEVRKRKIETYANIDMDHIISAPDVESVYSIPLNFEKENIGRKILAMFDLPERR
                     EPDWANYSTLVKRILNPRETVRIAMVGKYVTSGDFSLTDAYVSVNQALEHAGAANDTK
                     VQIDWVSGADFNDSNVSDELRAYDGILVPGAFGSSGAEGMIAAIRFARESGIPYLGLC
                     YGLQMAVVEYARNVCGLARANSREIDPNTPHPVIDIMDLQRAVIEPTVWQAWRAISQQ
                     IDIVADNWLNSRQHQIGGSMRLGAYAARLIEGSLVFRLYQESGRLESDRSRMDRLRMQ
                     PGQAFRLGREKPGAPVVLERHRHRYEVSPAYVERLERGGLVFSGYHEQADGNRLMEFL
                     ELQEHPYFVATQAHPEFRSRLEEPAPLFVGFVRAALERKHSAGK"
     gene            10963..11628
                     /gene="lptC"
                     /locus_tag="PLT86_00590"
     CDS             10963..11628
                     /gene="lptC"
                     /locus_tag="PLT86_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOT35146.1"
                     /translation="MKSSPVLIVVLLAQAVVMAACDDIGSSRRNAPEAALSGQRLERS
                     RIVWSDRGRKSAVVSAGELIRFDNRNEITLRDSVVVEVYDETGALVAIARGEEGTVDE
                     RSETARVTTGISVRFSGNDGKETSTLTARQAEADNRAQTVSASGNVRVRSDGGVTVYT
                     EKVIWDGRTRRFRAPGFVRLVNGTDVEEGENLDANSDLTHWTMDRVRGRSTRVREELE
                     SGS"
     gene            11631..12899
                     /locus_tag="PLT86_00595"
     CDS             11631..12899
                     /locus_tag="PLT86_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOT35147.1"
                     /translation="MAARTTTLATLFVGLVVCGTSGAQQGQRAVEFSADRTEMIRRGD
                     RFLKRYTGAVVIRFGPGEWLTADEATYDSDGRNINIAGSVVMQDSGRILRADRVQYLF
                     RDNPRAGGHPLVLLLGRVALVDNARRLNADRAEYRSHADSLAAFGDVVAKDSSRTVTA
                     DTLLLKADGNRFVAMGRVVLEDTVENVTVRAGYYSFDGADSVGVIAREPTLVRGAGDT
                     AVTVRADSMILNVTGKKGSAWGTVRIEKGQMTAECDSLALDDNRGTIALFGHPHAVQK
                     RQEDSTSTVDHLWGDRIVLEVEQSKITRILVDGSARTSSTRSDSRGGLGPERWATGSQ
                     MVFHVEHDEVREVIVWGQARSRYEPDFEDARESGTNEAAGDTIRVAFRDGKPVRVHLA
                     GGVHGLYVQNVARRTDATTRAASPDERRGG"
     gene            12892..13635
                     /gene="lptB"
                     /locus_tag="PLT86_00600"
     CDS             12892..13635
                     /gene="lptB"
                     /locus_tag="PLT86_00600"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOT35148.1"
                     /translation="MDDSEIRSSLRAEGLSKRYGGRLVVNHVSLVVKRGETVGLLGPN
                     GAGKTTTFYMLLGMVVPEEGRVYLEEREITKLPMYRRARLGIGYLSQERSVFRKMTVE
                     QNILAILERLPLTREERMQRLEEILDELDIARVRKTRGEQLSGGECRRTEIARALVIE
                     PKFLLLDEPFAGIDPIAVEDIQSIVARLKAKGLGILITDHNVHGTLAITDHAYVMAEG
                     GILCAGTPAELADNELARKFYLGERFRLN"
     gene            13676..15073
                     /gene="rpoN"
                     /locus_tag="PLT86_00605"
     CDS             13676..15073
                     /gene="rpoN"
                     /locus_tag="PLT86_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOT35149.1"
                     /translation="MRQELRLQQRLAPQLIQSLHLLQLPALDLEQVVREELETNPLLE
                     EVPAGELEHGKEEPAGETGKEADATSDIDLDATEWSDYLSDSYELSQTFREEPDHSAE
                     TYEPVSVYQETLADDLREQLRFVLSDETELKIGEYIIGNLDSNGFLATTVEEIAATLG
                     VPAAQVQKVLKAVQTLDPPGVGARDLRECLLIQLRERGYEEDPAFEIVDKCFDELMHK
                     RTKEIMRITRLSKEDVAEALEVISTLNPKPASVAYGSSERPVTPDLIVERVDGEYVVS
                     LNDRSIPALRISQNYRELLAGGGTEETKKYIVDKLNSAKWLIKSIEQRRTTMLKVMNA
                     IVKHQRAFFDYGLDHLKPMVLQEIADEIKMHVSTVSRVSNGKYVQTPHGVFELKFFFD
                     GKLGVSDGEDMASKVVKEKVKQMIDAEDKQNPLSDQAIADRLTRESGIEIARRTVAKY
                     RDQLRIPSARMRKEV"
     gene            15075..17477
                     /gene="sppA"
                     /locus_tag="PLT86_00610"
     CDS             15075..17477
                     /gene="sppA"
                     /locus_tag="PLT86_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT35150.1"
                     /translation="MSFDRFWSPVVLSMLFSGSLFASTPFVTSTDRFRSQLGIIENPA
                     LSDEASAVWVNPAGLGVRTSATMFSSVAMRNNPWYANLLFAGNGSGFGWQRTDAGSGQ
                     RIDRWRFGGSGGSSPYGLSFGAAVELSDPDGLNENLFWSGDLGVLARPVTWMSAGLVV
                     RQLGARRGYPWSVESGLALRPFGPNLSIFGGLAYCEDDPLNDPSHWHAGALANVGPGL
                     EAYGAINQDRTILVGVQMILGRGSIGGAGSRVSGGSVGSGWVIARSHADYRSNRLAMK
                     GRIAEIRLKGEIRDQTPGFSLFGNRGTTLSELVMQINRAAQARDVGGLYLRFDNLAIG
                     QGMAEELRDALIKFKANSGKPIVAYLPEASFREYFIASVADSIFLEPVGDLRLTGYGV
                     GQLYFRRALDKLGVEADFTRIGRYKSAAETFTDSTMSDATREQYEELLDDWYTRTVDG
                     IAVSRRLSADSVKALVNNAPYMAAEAVRVGLIDSAGHSDRAYESVETMVRSREGRVSG
                     KINLARRRLYDETWGPRPKLAVIFASGQIVNGTSGEDFFSGTQMMGAETIAKALKQAR
                     EDDAIKAVVFRIDSPGGLALGSDIIWREVQLLWETDKPVVVSVGDLAASGGYYIACRA
                     DTIISNPGAIVGSIGVFDGKMVVERLARRLGIDVELLARGDNAAINSSLASRTPEQRR
                     RVAENVREVYDVFVNRVATGRGMEAASVDSIGQGRIYTAANAVSIGLVDKLGGLDEAI
                     RTAARMARLRGEVELVTMPRRTNVLETVIQSSLQDAMGVSTRQSLAGEVYFFDPVAAS
                     LR"
     gene            17555..20671
                     /locus_tag="PLT86_00615"
     CDS             17555..20671
                     /locus_tag="PLT86_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT35151.1"
                     /translation="MRDVVTGTTGFAAPAAKSLLAGAIVLCLMCSHAHAQVGEFGRNK
                     PTSATGNWHYVQTPHFDIYFYEGMDAIARIATTIAEEAYSQISASFDYRLRNRVPIVL
                     YRSQGQFSQTHITPSLIGEGVGGFTEFLKNRVAVPFTGSYPQFRHVLHHELVHAIVHD
                     MLYGRDILTGVQQSDIPLWFIEGLAEYESSPWTSESDMWMRDAVINDYLQLDGYFAYK
                     GGQSIMYYIAEVYGPDKVAQILNRLRSFSRFGPTVQAVLGVELEDFGKRWEKDLKKVY
                     WPEIVDRDEPEDIGQSLTDHTKDNSYFNRNPSFSPFGDKIAYLTGRDYYTSIYVMSAI
                     DGKVLWKVVTGETSGSFEEMHWQRAGIAWSPDAQRIAFAAKATSGDRLYIVDARNGRR
                     LDAFDFGFDGMFSPDWSPDGTTIVFVGVRDGKSDLYTLRLSDRSIDRLTDDYFDETDP
                     KWSPDGRRILFASDRAPDVSDVSAQLLYQTSDVYVMDVETRAMRRLTADPADDLSPAW
                     GPEGKHIAFVSDRNGIFNLYVAALDSGEGSIDSATRPLTNILTGAFAPSWNRRGDKIA
                     FSGFNKGGWDVFVIRNPLDSLREEPLRPAYFLREGKISYSSRHRHLATSTQSATSPDS
                     TAGFSIGPRTSPFGSPALDILAFTRADSARGGFVPKPYRSQLQVDNVSGQANYSTLGG
                     VGGQLYVTASDLMGFHQLQFNTSLYRDLRDSDYGVFYQYLKRRTDYAAYFQQQRTWYP
                     FQSVDYYGGIVESGLVESREISTGVYASRPTSQFRRFEAGIDYVNAKQSVLYDYLSYG
                     TGIAASQEATIEALLTRVAFVHDNALWNMFGPVVGQRWRMTVFAAPPRANTAPFGVVT
                     ADVRRYWRVSKNTSFALRGSGGRSFATHAQNAPLFYVGGLPYWINYDYENVQSSQLVN
                     DAFSQFVFPLRGTNYYELRGRSFAILNAEYRFPLIYFLATGPLNFVLSNVGGVVFLDV
                     GRTWEDPTQPSDPNIARHAQGPLGGYGYGMRINLGVLVLRMDVAWRTDLRETIGSARY
                     YWSFGYDF"
     gene            20759..21592
                     /locus_tag="PLT86_00620"
     CDS             20759..21592
                     /locus_tag="PLT86_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HOT35152.1"
                     /translation="MEDSARTPAHFLSPDLPFVTNRHAEVLRRVRVVLVGPKYPGNIG
                     SVARAMTVCGLTRMHLVNPVPSWRESDEAWTFACGSRSVLEQATEARNLEEALCGATT
                     VIGTTHRMGRGRGPLLPPRKLAEHLPEMLSKGEVALVFGREDKGLSNDELALCQMGVH
                     IPTASTYPSLNLSHAVMVVAYECFYAVVTTGDENVHVSGVSYPEIVALSERIAHLTAR
                     TGFRHRRGEVGFERSLRRAIERSSLSARDIALAHLVCRQVEQYIDRLEEQIRNERETS
                     N"
     gene            complement(21675..22013)
                     /locus_tag="PLT86_00625"
     CDS             complement(21675..22013)
                     /locus_tag="PLT86_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT35153.1"
                     /translation="MKKVEAFIRPHLVEDVRTALTDAGVKGLSVLEARGFGHQRGHTE
                     FYRGAEYQVLFVNKVKIEAVVLDDQIDAVVSALLRVARTGKVGDGKIFVSTVDDAIRI
                     RTGESGNAAL"
     gene            complement(22053..23336)
                     /locus_tag="PLT86_00630"
     CDS             complement(22053..23336)
                     /locus_tag="PLT86_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT35154.1"
                     /translation="MNRRLMAFLTLLLGIVPPAFAEGSGSIDSGDTAWMLTSTALVLF
                     MTPGLALFYGGMVRSKNVLGTIMHSVFAMGLVGIHWVVIGYSLSFSKGNSFIGGFSHA
                     FLNGVGVEPMGSIPHLAFVAFQGMFAIITPALITGAFAERMKFRAYVAFVLLWTTVVY
                     APVCHWVWASDGWLFKLGALDFAGGTVVHMSSGFSALVAAIYIGRRVGYPKDQILPNS
                     LIWTVLGAGMLWFGWFGFNGGSALASGALATLAFVTTQIAAAAAAMAWAFAERIRTGK
                     VTVLGVASGMVAGLVAVTPASGFVGPMAALAIGLAAGVICYWAICLKPRFGYDDSLDV
                     FGVHGVGGTLGALLTGVFASTYWNANGKDGLLLGNVDPLLVQALSVVATAAYAAAATW
                     IILKLIDIAGGVRISQAEEREGLDLTQHGETAYHL"
     gene            complement(23541..24482)
                     /locus_tag="PLT86_00635"
     CDS             complement(23541..24482)
                     /locus_tag="PLT86_00635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT35155.1"
                     /translation="MLRLVDHDTLNAIMRRLSLAPVVSSHPLTAPANRTGSRMLVLES
                     GERWAIKFDDSPTSDGQGWWREYVGWRRARRAGIPCAEEVIVLPPEEPWNCGVCCVKQ
                     IPGVTGDLMLNSTPSLLPVALRSVGKAMAEWHKAKTLAPEELFTPFFDHLVSKERFAQ
                     SFEARAVQLAESGLVSQTLRARLIFFLDQNLHRFDENTLVPIHGDANIANMLFDRFTG
                     ALSGLLDFETLSVGPARYDLAPLYGAMSCAYYTKRTFVNDPDWMFLWEGYTGTTEFRR
                     EDRLELTLYTLDALLHAPFVPDYWYSVFASLILDGIA"
     gene            complement(24482..25600)
                     /gene="lpxK"
                     /locus_tag="PLT86_00640"
     CDS             complement(24482..25600)
                     /gene="lpxK"
                     /locus_tag="PLT86_00640"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOT35156.1"
                     /translation="MRRIDEWLLNPPRTKLGKSLMNGLAAASTVYTAGVRARSWAYRS
                     GVFRKTTLNTPVISIGNITVGGTGKTPLAQFIARHFLDTGRRPVIVSRGYRKEGATLA
                     VVSDGKRLLLSPRRSGDEPYLLARTLPGVPVIVSPNRALAGFVAVSRFAPDVVIMDDA
                     FQHMKLDRNLDIVLVDAVNPFGNEKTLPGGTLREPPPNLARADVIVVSRSDQTDRLSE
                     LRERLRSLNPSAPVVLGVHAPDGLLVHPTGDSLDLTILRNARVLAVSSIGNPWSFAES
                     VRTLGAEVLDHLRFPNHAKYRHRALSRIADAARQLRCEAIITTEKDAVRLPSHIDCGC
                     PIWVLSIRFRIVEGAASFLDLLDALVTRAKTMGMNGQA"
     gene            complement(25614..26705)
                     /locus_tag="PLT86_00645"
     CDS             complement(25614..26705)
                     /locus_tag="PLT86_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOT35157.1"
                     /translation="MWHGASAGEVRGLRPLWEHFGGVASVDVHLSCTRTGVAMWSQFG
                     IECTHIRLFPADSAASWASMCRKQYPALVIVSETELWPGLFHAMVQRRVPVLLVSARV
                     SQRTTTLMKASRLLTDVAPALFVCAQTGDDAARFSSLGVPCEQTAVCGSLKWCRGSLA
                     NRADARAAMQMDGDDRVVVFGSVHREEMSHVVSAIEQIRLAGVSSRFVVAPRHMTDYD
                     YADTILKRGGLRPLRLSSLAHTGILTPESVLLVDKMGVLAQLYAGADVAVIGGAWVPL
                     GGHNPFEATAYGLPVVYGPHMEQAGCNLLEASGQATRAHDGRMAGAIIADILRNYPGP
                     FQSVIPDALAATVEAMARWRLPSRSKAHD"
     gene            27217..28473
                     /locus_tag="PLT86_00650"
     CDS             27217..28473
                     /locus_tag="PLT86_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35158.1"
                     /translation="MSGTIRVAIVGCGGITSAHLNGIRILQQSGLDDVQVTLLCSRDL
                     ENAARYNKRGDAPPPLPPIVPWRDDPLNIRDVYVTDLHPGVPAELCTDWRKAVVSPDV
                     DAVIILASVGVHHTIGCAALGAGKHVLIEKPFAITVRAARKLCEIAHERGLSLGVAES
                     LRYKRETRSARWALDNGYIGKLDMVAHVSLGSVWSPDKIVGRTPWRHHKLETGTGILM
                     DIGSHLFDKVRYVCGEFDTISGITRTLEPFRTTRDDAGHIIAQTHCDVEDTAFATAVF
                     KNGAIGTFSMSWATHGEPASLPGGTALFGDLGCIKEGKLIGEREGVRVLEDLFVRRAP
                     KGLQEEWFPRGVTDAFALELHEFFRSIREGRSPETSGIEGLRDVAACYSVVESSVRGE
                     CVRFEDVANCRVETYQRAINDQLGLG"
     gene            28504..29139
                     /locus_tag="PLT86_00655"
     CDS             28504..29139
                     /locus_tag="PLT86_00655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT35159.1"
                     /translation="MRHGQTEWNVERKWQGVTDIPLNARGRAQAAAVARMFSSSGMLG
                     GVAIRRVVSSDLSRAKETAHEIARVALVPLETTPRLRERSFGRWEGLSRSEIAQQFPG
                     DIEAYETQRETFRPLGGQSWQEFSEQAARTVHELVGDFAGQSTVVVAHAGPLQAFLSQ
                     VMGTRDSRPGRFMIDNGSVSVVREWLNEHTGDRHWVVTALNITCHLHALES"
     gene            29154..29801
                     /gene="tmk"
                     /locus_tag="PLT86_00660"
     CDS             29154..29801
                     /gene="tmk"
                     /locus_tag="PLT86_00660"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT35160.1"
                     /translation="MPGLFISFEGIDGCGKTTQVERVAEIIRGTGREVVSVRDPGSTR
                     VAELVRNMLLDRGNSDIDARTELFLYLACRAELVERCIRPALGRGQVVVSDRFSDSTL
                     AYQGYGRMLGVELVRQANRIATAGLTPNVTLLIDIPVNLARSRRGAKIVDRLEAEDGD
                     FHERVRNGFLEIARREPDRVAVLDGTLPVDDVTNAIVDTLALRVPVLLGDVIKSS"
     gene            29892..31712
                     /locus_tag="PLT86_00665"
     CDS             29892..31712
                     /locus_tag="PLT86_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT35161.1"
                     /translation="MALRRRDWGWLALMVAVVIWGGQIFNHVEALAQDAYGRIGQMAD
                     DRAEVETAILRFYVEDVDAGKLQLSQVDGMLNELDTYSDFLNEREQEDLRMTTEGAFG
                     GLGIEINAVDHYPTVISPLEGTPAWDAGLLAGDQIIEIENESTYDVKLDIIVGKLRGV
                     PGTAVNIKVRRPGSSEPIDFRITRAEIHVNSVQFHGLLWDPRAEGFLPLGAPDWLRNA
                     QGIGYIRLSSFSRGATDEVRVALQDLMAKRARGIVLDLRMNPGGLLEEARGVADLFLP
                     KGRLIVSTKGRAQRGEQRLYSERDPAIPPSMPVVVLVDEGSASASEIVAAAIQDNDRG
                     LIVGRPTFGKGSVQTVYNSQFSGRFGLSFSQNALLKLTTAYYYSPSGRCIHKPRWRTG
                     KRGAVVSKAASDTATVYRTSKGREVRGGGGVAVDVAVQPDVPPALFWQLNARQLFFNY
                     AVDYAVKHPELDPQTFDVNEAMLSDFRRFVADSVHAFTYEPRGKKELDDFAKATSEAG
                     YSQEVTRRVEELREALLAQKDAEFSRAEPYIRQRLRSAIAGRVWGRQSRTRADFTRDL
                     ALKEALTYLTDSGRYAQQIALSPVRPRPQQQEQTAENEGD"
     gene            31834..32439
                     /locus_tag="PLT86_00670"
     CDS             31834..32439
                     /locus_tag="PLT86_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT35162.1"
                     /translation="MELANQSDSELISMFLDGQAAAFDTLVRRYERQVFGFIFGMVQN
                     EALANDLFQETFIRIINALPDYRERGKFASWLLGIARNLALDALRKRRVEGRIFRLEN
                     RTTDSGNAESTYPEIPDDIESQADMVEREEWLVRMREALAELPVEQREVVYLRYEADL
                     TFQQIADLTGVSINTALGRMRYALNSLRKRMNVATEPSTNE"
     gene            32432..33058
                     /locus_tag="PLT86_00675"
     CDS             32432..33058
                     /locus_tag="PLT86_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT35163.1"
                     /translation="MNDTKGTPACLRTQRDALRYTSGELSAEERITFEVHLQHCRECA
                     RLVDDERTIRRVFGALPRSSPSATVRALISERAAASGAAYHKLSSPGRIAPLRTALVT
                     RIPVWTWPVAAAAILLLGVSLTFHRDVRTPVFPSDTLSAPVALVPDVPAGDGIQVASV
                     SETTKASTRIRTPRDGASASDNLLDDDVVELADRVETLAMILDEETTQ"
     gene            33232..33690
                     /locus_tag="PLT86_00680"
     CDS             33232..33690
                     /locus_tag="PLT86_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35164.1"
                     /translation="MALEFIRRIDSSRVEQVIALKTSRPHAYRSMILRSLNMERHARA
                     MGRRGDPGEIRQSEQLFRRMFELREQMDRLSIQYRLATDENAKNEIRARFSAKAGELF
                     DVQTEMNRLQIEQIADELRRLQQRLEQRQAGRDAHIRRQVEIMLSDEELD"
     gene            complement(33707..34213)
                     /locus_tag="PLT86_00685"
     CDS             complement(33707..34213)
                     /locus_tag="PLT86_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOT35165.1"
                     /translation="MTEALHLPPLPPCAADMMLGRLATWLRLLGADVSYQSWISDADL
                     MERARNENRLVLTRDRGLLGLPGSTPRFGVTFDRLPDQLAQVAAHFDLTALVPFSRCV
                     RCNVPVKVVPKESAKGRVWEYVYDTQEIFTECPLCGRLFWKGTHPARAREDLRRMLGE
                     DLARRIRW"
     gene            34351..35370
                     /locus_tag="PLT86_00690"
     CDS             34351..35370
                     /locus_tag="PLT86_00690"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT35166.1"
                     /translation="MKEWDRRIAHPITGQENPAYLHGVIVPMFTPCNSNGTLDERGIR
                     SYVNYLVSNGSVSALFARSGLGRMYTFSVNDVRRLADIVIDEVADRLPVFVGCSGELS
                     ADSPERPDAHTYLRQSIELCQYVQRKGATAAVLVLPVALVPHREELPAEVVLEYFRRV
                     EAETDIPLIIYQPPETTRRFRATPSLIEEVSRSGQVVGMKISTSRMDVFLSCSKAAQN
                     ANFALIAGDETVFLFTILVGGTGVIGQGCTTHPEILKAVYERAMAFDLAGASKAALDT
                     FAAVNAAPGETLDGVVFRYLQRQGVTVQPYSRDGGRPPSEEISARFASSLDAVRSGYR
                     DSMVR"
     gene            35403..36449
                     /locus_tag="PLT86_00695"
     CDS             35403..36449
                     /locus_tag="PLT86_00695"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT35167.1"
                     /translation="MKRWTDPVVQPYTGRVIPAYLHGVITPMFTPCNPDGSFDEKGIR
                     AFVDYLIGTGCITTLFARSGLGRMYSFSYDDVKWFADVVLDQAAGRIPVMIGTTGIWG
                     RDFHHRPGNEIFTRQSIELSQYVQKKGAVAAVLVLPMALQPEEGRPLADTIFAYYEAV
                     AREVDLPIILYQQPGLLEEYCMTPPLLERLLTIKNIAGMKYSTGDMVKFSGLTMVARN
                     TDFAFIAGDELAFFFAMMLGASGVIGQGCDVNPETLRAVYDRMMARDFAGALEAHYDC
                     IRAGQISEGYDCVISGFRYVAAKGVRVQPFTRGHGVSDYQVQQPVVPDDWMASFSREM
                     DALRRKYPPVNPWA"
     gene            complement(36507..37433)
                     /locus_tag="PLT86_00700"
     CDS             complement(36507..37433)
                     /locus_tag="PLT86_00700"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT35168.1"
                     /translation="MTGTTLSPDLRTSVGALDLANPVLVASGTFAYGREMARLYDLSL
                     LGGIVSKTITELPRAGNPPPRVCETVGGMLNSIGLPNPGVDAFLRDALPELRQAACPV
                     IVNVAGETAEGYVRLAARVAREKGVSAVELNVSCPNVSHGLDFGTAPSLVEQLVRRVK
                     QECALPVIAKLSPNVMDIAEIARGVEQGGGDAVSLINTIRGMAIDARTRKPVLGNVTG
                     GLSGPAVKPVALAMVWKTSRSVRIPVIGIGGIMTGTDVVEFMLAGASAVQIGTGNFVD
                     PWVAPRVISELRDFCIQEDIASVRSLIGELKA"
     gene            37638..38891
                     /locus_tag="PLT86_00705"
     CDS             37638..38891
                     /locus_tag="PLT86_00705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT35169.1"
                     /translation="MDAKKKARVFVQTAAAPLSELDRWVQVGEEEARAVYEMTLQNEL
                     SGGTEQVRAFENEFREFTGLKHAISVINGTAAIWSAMYGCYVGPGDEVICPVYTWLCS
                     ITPAPMLGAKPVFCDVSPHTLLMDPEDVKKKITSRTRAIVAVHLWGMVCDMQALGAIS
                     QETGIPIIEDCSHAHGALYGGRVVGAIGKVGTWSMQGSKAVSAGEGGVLATDDDEVFD
                     RACMIGQVNRAAGLDLVSAKYQKYQPLGLGIKFRAHPLGIGIARVQLRKLGLLNAGRG
                     GYIEAVERALKEIPGLDPVTLTDGTKRGGYYGFPTIFRAEQVPGVTRDQYVNALRAQG
                     VPASSSPYDLLHRLPYFAEGMDVFGFGRGVMCEGYNGYKEGDFPGAEEMHRNVVFLPV
                     LTDPVEGATEEIIRRIRKGVESLGF"
     gene            38881..40065
                     /locus_tag="PLT86_00710"
     CDS             38881..40065
                     /locus_tag="PLT86_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT35170.1"
                     /translation="MDSKDHAATPPRSEDSWIVFSSRRTEYACAEIGFVRPDGTGLHF
                     PHLDIEGQVSWHAGPAFAGGDRIILHSVENSETWKHRSRSNLWIYQISSGELTALPLK
                     HRPADFMPPALLLPGDERIVVNPVIQGNQCVVTMNLDGSDPVHVTKPEDGFAYGIALS
                     PDCSMLAFHIAGPPNYFACVADIDGANRRVVARDPDVLFFAPMWSRNGDWLVCEGCFS
                     GKDPEHFAADLYLVRPDGRELRPVTSGQSHWFAAAYGDEKSRSGGSNVSRWSPNEDVV
                     SYTRLAPGSRTAWKHIEQPQEDDHFNRAYAPEEARGGTQLCALNPFERACRPLTEVRE
                     GVWDMRAAWSSDGALIAFCRAHVGQPPGLWVMSADGGQARLLTVGKEGLGADHPAWIH
                     AR"
     gene            40122..>41311
                     /locus_tag="PLT86_00715"
     CDS             40122..>41311
                     /locus_tag="PLT86_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35171.1"
                     /translation="MEPQESGRGPVDWYTSDPWKAYLGWLSPRWAEPREDFDPEEWCD
                     ALVEGGFQTAVVHAKHHDGICFFPSRFRSAQPPRDFFGEIVGAAKKRGIRIVAYYSTT
                     FDTWTWEEHPEWSCREQDGSVTVLTRFPFPIGVCCHVNPGYRQFLLGQLEEIQGAYDT
                     DGFWMDGFDYTGYPSRACFCHFCRERFAKDNAGMSLEAAYLSDKLRLWQRDVFLELMK
                     DIVTIANRGRADRVVVYNNAGTNLELGYERIDELCSLNSMEAHSALGKSLTARLLAGQ
                     RKAFEIYTPVADKVFSWTPRTTPLLKLETAIVAAHGGSTLAGLDVTASGRIPGFQTRQ
                     MAEVGAFVRRVAGVCHEAEPVYDVGVLIPKDQWKDERGTWAVCLLKHHHAFCQVPLHV
                     RDYS"
BASE COUNT         8831 a        11244 c        12825 g         8411 t
ORIGIN      
        1 agatggacga ccggacataa ttgcttcaaa ctcgtacgga gaagtatggc aatggctctc
       61 ggcatctcca gattgctcct gggtgttcgg tgcgggaaca cgcgtgcggg ccggagggaa
      121 gctcatacgg atattcgccg gagcctcggg aagtatacag gggccgggag aggcacggta
      181 cggatacggg gctccggaag tggcggattg gaacggggac ggcgcgctgg atctcatact
      241 tgctgacgtg tgggggaaac acacacttta ctgcggactg ggcgctgatt tgcgacggac
      301 ggacgcaaat gcgggattag agctctcaga gggacagccg ctgcgtttcg cgaacccgcg
      361 agacgtcgta aaaccggcgt ggaattggtg gaatcccgtc ggtggagagc ttgtgacgca
      421 gtggcgaaca cgcggtgagg tgctcgattg gaacggcgat ggcgaggtgg attacgtcac
      481 gatcgatcac gaagggtacc tcgctcttgc tcccggtgtt cgcacgcggg aaggcagaat
      541 gctcgaaccc cttgagcgag tgtttctgaa tgaagccggg gagccgcttc tgctcacacc
      601 gggaacctgt ggccgcagcg gccgttacaa aatcgctctg gccgattggg atggcgacgg
      661 ggatctggat cttatcaggg gtgttgcaac tccctccgga aataccatgc acacgccttc
      721 cgaagaaacc ggttgcgcat tcctgtttga gaacgtcggg aataaccgct ttgcgttcag
      781 gggggagatg gttcccgacc cgaccatcca gcttgccggt cacagttcgt gccctcaacc
      841 gttcgacttt gaccgcgacg gcaagctgga ccttctaata ggagctgaag atgggcacat
      901 ttacgcgttc caccgctcct atctcgaaaa ggatctgccg cgggttcagg taacgcgggt
      961 cagggtgacg cactgagaga ctggcgcacc gcaactccgc gcaggtgtga atcaggcttt
     1021 tttccgtttg agccactgaa ggcgcgaacg gacttttgcc tcctcgtttg cgggtaatgg
     1081 ttttacaggc acgtgcggtt cctcgaccgc gggaagcatg cgagtgtttg acggcgccac
     1141 gcgttgcttg ggtgtgtaag gcggtttttc caaaaaatgg tgcaacagga tcagcgccgt
     1201 ctccttgtcc ggaataatgt cgcgctcacc ggctgcatcg tggccgcccg cagggggcgg
     1261 agctcccacg caggacggac agccgtcgat gcaggggcac tcggaaacca gttcccacgc
     1321 ggccgtaaag atctgctccg tccactggta ggtcctttcg gaaaaaccga ttccgcccgg
     1381 gtatttgtca tacaagaaaa tcgcgggcgt gccgaggttg gagctgtcaa caatgcttcc
     1441 gatatcagtg gtgtcggcca tgacaaagag cgggacaatc tccacgagaa cgttggcaat
     1501 tcctttcaag ccttcagcgg gaactctgcc ccaggccctg cattcatcga gagccgcagc
     1561 acggggaaca agccagaatg cagtcgtgtc aagcgtctgc gtgggcaggt caaggttctc
     1621 gtaaccgatt gactccctgc ttccaaactt gattttcttg aacatcacta cgttgagact
     1681 tacggtgaca tcgccgagcg ccaatttgct cacacgccag gcaccctccc gttcggtgtt
     1741 ccgtacgaca atgctcgact cgctgacaga ttgcgtgtag taatccacgc cttctcgccg
     1801 aacgtgcgcg attttgcgat ccacgtcgag tttgtcaaca aagaaggtct cggcatcgtg
     1861 gaggtagacg gcgtgagtgt gtacctggga aagcgcgctt atctcgtcga gtgtgccgat
     1921 tgcctgaggc ccctgatccg tttcgttcat gatcgtgtac gtcgtgtcgt ccatgttgcg
     1981 aagaccgatt ttcgcggcgg cataccctga tctcgcgaag tgccagcgtt caccgacccg
     2041 ccgaacatgt ccctcgtctg caagtatttc caccaacgca ggcgtgtact caccaaacag
     2101 atcaacacgc tcttcggggg cgatggggag ttcctgtgcc gcacagagca gatgaccgat
     2161 cgctatgtgc ggattggtgg gatcgatgat ggcgtgttcc ggatttcttt cgaagaaata
     2221 ctgcggatga ttcatgagat actggtctat gggcgtattg tgtgcgacca gcaccacaag
     2281 ggaacgttct ttgccccgtc ccgcccgacc agcctgttgc cacgtgctgg caatcgtgcc
     2341 cgggtaaccg atcagaatgc tggcctctag cgtgcctatg tctatgccga gttcgagggc
     2401 gttggtgctg atcacgccga gaagttcacc ctcgaagagc tgccgttcga tttccctacg
     2461 ttcttccggt agatatcccc cgcgataggc tctcacccgg cgcgcgagtg acgcgttgcg
     2521 gtgagaaagg acatcctgga cgaaacggta cagaagctct gcggagacgc gtgttctgac
     2581 gaaagcgatg acgggagtcc gctcttcgat caaggacacc atgcaatact gcgcgtcgga
     2641 gagcgcgctc ctgcgttccg gagtctctcc ggcggacgga tcatccagca gaggggggtt
     2701 ccagagcaca aaactcttct cgccgcgggg tgatccatct cggtcgataa gcgtcagact
     2761 tcgctgggtg aggttttcgg catgctccag tgggttggcg atggttgcgg atgagcaaag
     2821 atacagcggt ttcgacccgt agtgcgcgca gatgcggttc aagcgtcgaa gcactcccgc
     2881 gacgtttgaa ccgaacacgc cccggtaggt gtggatttcg tccagaacga cgtatctgag
     2941 cttctcgaaa aaacgcgccc aacgcgcgtg atgaggcagt ataccggcgt gcagcatatc
     3001 ggggttggtg agaatcacct gcccctcgtt gcgcagaacc cggcgtgtcg taggtgccgt
     3061 atcaccgtcg tacgtccccg ccttcacccc caatccgagc ccctggaggt aacgcaatac
     3121 accgcggagt tggtcctggg ccaatgcttt cgtcgggtag atgcaaagcg cgcaggagtc
     3181 cggctccaca aggaggcgct cgaaaatcgg aagatcgtaa caaagcgttt ttccgcttgc
     3241 ggttccggtg acaaccacga aaccgcgccc gcttctcgcc gactccagcg catcagcctg
     3301 atggcagtac aggcggctga ttccctgggc tttcagcacg tcggcaatgg cggcatcaag
     3361 gggtacgtcg ggttccctgt acgtcgcttc gcgagccggt atccgttgta cgtgcacaac
     3421 ttgccgacgc catccagatt cggctccaat acgtttcaaa aacctgtcta catccatggg
     3481 attacccttt gttcgtatgc aaagagtccg acgggggctt tgcggactct gttttcctgc
     3541 gttaacgtac gcaaggaaac tgctggaacc cacagcccgt gcgcgttcgc tttgccgatg
     3601 tggggaaccg acaccggacg agcagaagcc aaggtaatat acagatctgg gaagggagcg
     3661 cggccgtgaa aggcgtcatt ctggcagggg gaacgggttc ccggttgttc cccctcacca
     3721 aggttaccaa caagcatctc ttgcccgtat acgaccgccc catgatctac cacccgatcc
     3781 aggcgctggt gaacgcgggg atccgggaca taatgattgt gacgggaggc gagaatgccg
     3841 gtgatttcct gcgccttctc gggaatggca gcgaattcgg cctgcaacat ttgaactacg
     3901 cgtatcagga gaaaccgggt ggaatcgccc aagcgctcgc tctggcggag cattttgtag
     3961 ggaacgagaa gttcgtggta gccctcggag acaacatcat ccagaagagc attcgcgcgg
     4021 cggcacaggc atttgagcac cagccggaag gggcgaagat cctcctggcc gaagttgaca
     4081 acccgcgcag ttacggaatc gcacgtattg acggtaacgc cgtagtcgag atcatcgaaa
     4141 aaccgtcgga tccccccagc aatttcgcgg tgataggcat ctacttctac actccggagg
     4201 tgttcaaaat cataaagacg ttaaagccgt ctggccgagg cgaactcgag atcacagacg
     4261 tgaacaacga atacctgcgg cgcggtacgt taacgttcga gtaccttgac ggatggtggg
     4321 ccgactgcgg cgaatcaatc ccatggctcc ttcgcgcgaa caatctcgta gcgcgcgacg
     4381 gcgcgaacaa cgcgggatag gctgatccac ccggcgccga agcgtttgtg caccgcacag
     4441 gccggacgtc cagcccgcgc accattttct ttgaatgatg gatgaattgt cttcgcattt
     4501 cccgggacca gcaagaacac atcgcgcggg gaggcacacg catggctgac aaaatccgga
     4561 tggggttcat cggatgcggt ttcatgggtc aactggccca catcgccaac tacgcccgtc
     4621 tgcatgacga atgtgatctg gtcgctgtcg cggacgttcg ccctctgctg gcgaaaaagg
     4681 ttgctgcgcg ttacggaata cgcgaaacgt acaccgacca tcgcgaactg ctggaaaaag
     4741 cggatgtcga tgccgtggca gcgatcatgt attacgggca tcattacgcg gtcgtccccg
     4801 acgttatcga atcgggaagg catctgatca ctgagaagcc catggcgtgc agtgcctcga
     4861 gcgcgcggga atggaaggcc gcggcggacg cgaagctcct ggtgtacatg gtcggataca
     4921 tgaaacgctg ggatctcggt gctcgatggg tgtccgacat ggttcgggag tggaaaacga
     4981 ccaacgagtt cggcgagttc actcatcttc gatgcctgat gagcggaacc gattggacct
     5041 ggaatcccta tcccgtcatc tcttcagacg aacaacccat gacctcgttg ccaccgcacg
     5101 agccattccc ggcccgtttc acgaaagcgc aggccgatta tttcaacatg aacatcaatt
     5161 tctacgtgca ccaggtgaat ctgatgcgtt ttcttctcca cgagcagatc acgcttgatt
     5221 attgccatcc agctgggaag gtactcatcg gttccacgga aagcgggaaa gcagtgtcac
     5281 tggaaatggg gatttccagc attccccaga cgtgggacga agtctatcag atcgcgtttg
     5341 agaaagcaga catagaactg cgaatgccgg ccccgttgcg cgggcaacac aacggcgaag
     5401 tagtggtgcg gcggacggtg aacggaaaga cgtatgagac gctcagaccc aatttccctg
     5461 ttccgagctg gagtttcctc gaacaggcaa agggattcct ggaggcagtt cgaaccggca
     5521 gggttccgtt cgattccacg ggtgacgcag tccgcgattt ggagtttttc gagacccagg
     5581 cggacatgat gcagggagaa ggcggagtcg cccgaaagtg aatgcgtgaa ccaacagagg
     5641 agggggcggg aatatggttg tcgagctgga aaaaggagaa ctgcgggcca cggttgtcga
     5701 caacgcggcg catctggaac gggctgggca ccgggagggc tacaacggcc tggcggcact
     5761 caccagcgac gcgcagcagg cgagccctat cgtccctttc tacgctggaa tgaatctcga
     5821 gctctatttc gacggtgtgg tgaccgaccg gtcaacactt tttgaaccgc ggcgtgctcc
     5881 gatggcggtg gagaaaatcg atcaggcgac cgcgcggctc caccagccac ctgttcccta
     5941 ctggaaagtg gagagctgga ccgaattcac actacgggaa ccgaattaca tcgacttccg
     6001 ttatcgcgca atccctcacg agcggacgtt tgagcagaac tggctgggcg tgttctgggc
     6061 aacttacgtc gcgtacccgg aggatccagg gatctggttt cctactgcaa ccccggaagg
     6121 cgcgaaaacc atgcgacatt tctcccccgt tcacggtgac gaaagcacgc accggcatgc
     6181 cgcggacagc gaagagatta cggtgtcaca cgatcatcgg atttacatgt acggcagtta
     6241 ttcgcgattc cgctacgcaa gacctttttt ctggggattt gtgcacggaa tgagcgtgct
     6301 gttcatgttc gacgacaatc caaatatcag gttcgcgcaa tcgccatcgg gaggtggaac
     6361 ccgctgtccc gcgtgggatt tccagcttgt ggtgccggat taccgcgtcg gaggggagta
     6421 ttcacttgag gcgagaatgg tgtactcccg tctcggcgca acgctggatc ctgaagtcga
     6481 atacgcacgc tgggtggaat tccggaagaa taacagaagt gatgggggcg aaggtcgatg
     6541 aaagatcaaa gcgatggcgt gcagagctgg ccggagcgcg gtgaaagaaa cgggttgaca
     6601 cggtcaccga ttgcgggact cgatggtctt ctgtgcccgg tgtgcggaga aattgccgat
     6661 tcgctcccaa tcaaggttca aagagtgatt cagggtaccg aggaatgcct gttcgtgtgc
     6721 tcgccggatt gcaggcagat ggctctggaa tcaccgtttc tgcagatcaa catgttctcc
     6781 cgtgatatgc gggtctggga gagaagtgtg caatcggaac ttgacatcga atcttccccg
     6841 gtagccgagg ctccggaaac ggagcacaat gggagagaac actcgtgacg aaagctggga
     6901 aagcgatgac cgacgtggaa ctggttctgg acgcgaaagc ggatcttggt gaaggtgcga
     6961 tctggcatgc gcgtcgcggg ttgttgtact gggtagacat caacgccggt tttctgcatg
     7021 tgtttgagcc gaaaaacggg aaagacgacg cgtaccgcat cggcgaagac gtggggactg
     7081 tcgtgccgag ggttgcgagc tctgaacggc cctatgagag cgtaatgctt gcgttgcggc
     7141 gagggtttgc cgccttccgt ttcgatgctg caaaggcata tgtggttgcg gatccggaaa
     7201 gggatatacc tgcaaacagg ttcaacgacg gcaaatgcga tccggccggt cgcttctggg
     7261 cgggaaccat gccggaaggc cccggtggcg ttccagacgg cggacgcctt tactgcctgt
     7321 atcccgacct tcacgtggaa gtcaaagaaa ccggtattcg ttgctcgaac ggcattgcgt
     7381 ggagcctcga tcacaggaca atgtattaca tcgacacgcc ttcaatggag atctgggcgt
     7441 acaactacga cctcgaaacc ggggcgatat gggaccgccg tgtggtggtg cgcgtgccga
     7501 aaaacgaggg ataccccgac gggatgacga ttgaccgtga ggggatgctc tgggtggctc
     7561 actgggatgg atggcaggtt gtccgctgga acccgaagac agggcgaaag atctgttcgg
     7621 tgaaggtccc tgcagcccgt gtgacttcct gcgcgttcgg ggggaagaac ctcgacgagt
     7681 tgtacatcac cacggcacgg acaggcatca aagccgaagc gctgcgcaaa caacctcatg
     7741 caggcggagt attccgggtc ctgccgggtg tcagtggact cgaggcattc gaattcgcag
     7801 gctgagtccc gaacacgggg cgagcgttgt gagaggagat ccggggggcg ggcactgtga
     7861 cccgcctctt tccgtttttg ggtggcctca ccgtgcgcaa ccgacgacgg atttggcgtc
     7921 acgacaatgg ttgtgccgcg ttcacgccgc ttcgttatct ttaaaatggt gagcaaaaaa
     7981 cattccttca tgcacaaagt tgcgcataac gggcgctcca aacggtgcag cgtttgtttt
     8041 ggaagggggt tcgcagatgc cggtaagccg tgaggacgtg ctgcacgtcg ccgcgttggc
     8101 aaggctggaa gttgctccag aacaggtgga aagttttcag cgagagctcg gcgcgattct
     8161 ggagcatttc cagaagctga acgaactgga cacctccaac gtggagccga cttcccacgc
     8221 actggctctc gtgaacgtgt tccgcgaaga cattccacag ccctcgttaa cgcaggagga
     8281 agcgctcgca aacgctccgc agaaggcgtt cggacatttc cgagtgccca aagtcattga
     8341 ttgacgccat tttcgagaaa accgagacgg gtgttgccgg tgtggaaggt agtgggggtt
     8401 gttcctgcac gattcggttc ggagagatgg cccgggaagg tgcttgaaaa cgttagcggt
     8461 aagcctctcc ttcgctgggc atgggaaacc ctctccggag atcccgaagt ctcggaagtg
     8521 ctgatagcca cggacgattc ccgggtagag acatgggcgc gtgcacaagg aatgcgtgtg
     8581 gtgaagacgt cggcggaatg ccggaatgga accgagcgaa ccgccgaaat cgcccgcatg
     8641 atccgtgccg attattacgt gaacgtccag gccgaccagg tggcacttga gtcccgggtg
     8701 gtgaccgcaa tgcttcgggc tgcgaaagcc caccctgaat gggagatcgc gacggcgggc
     8761 acgcgctggt accgcactgg atgtctcgca aaccgagacc gggtgtttct tgcagtaagg
     8821 ggttgcacct gcggcaggca cagtattgcg gagcactttt tccgaggcgg cgagaagcac
     8881 acgaacaccg acagaccagt tctgcgacac cttgggttgt acggctatac cccgtccttc
     8941 ttgcgggaat acgccgcgaa tgagccgtcg caggatgagg tcatcttgca tttggaacaa
     9001 tgccgcgcgt tgcagatggg cttggagata ggagtagttc gcgttcggac ttctgcgcgg
     9061 agcgttgaca gcgccatgga cagggacgcc gtcgaaggat cgtggagcaa caagacaagc
     9121 aaaggagacc cacgtgagga cgcgtgaaca aacgttcccg aaatacatcg ttgttgcggg
     9181 aggagtcatc agcggcgtcg gaaaaggcct tgcaacctct tccatcggca ggatccttca
     9241 ggagttcggg tacagctgta ctgcggtcaa aatcgacccg tacctgaact atgacgcagg
     9301 cacgatgcgg ccgacggaac acggcgaggt gtgggtgacg gatgacggcg gagagatcga
     9361 ccaggacctc ggcaactatg aacgatttct cggaaaagaa ctatcgcggc ggaataacct
     9421 tacgacgggt cagatttaca aagccgtgat cgaaaaagaa cggcagggcg aatacctcgg
     9481 gcagacggtg caaccgatcc cgcatatcac caatgagatt aaaaggaggg ttaccgaggc
     9541 ttccgccggt cacgacatcg cgttggtcga ggtcggtgga acgatcggtg actatgagaa
     9601 tacgccgttt ttctttgcac ttaagagtct tgagcgcgaa atcggcaagc agaacatcgt
     9661 gtacgtgctg gtaacgtacc tgccggtgcc aacacacatc caggagatga aaaccaaacc
     9721 gacccagcac tccatcaaac tgctcagcga acaggggata ttcccggatt ttatcctgtg
     9781 ccgatcgaaa tcgccggtgg acgaggttcg aaaacggaaa atcgagacct atgccaacat
     9841 agacatggac cacattatca gcgcgccgga tgtggagtca gtgtacagca tcccgctgaa
     9901 tttcgagaag gagaacatcg gccggaaaat ccttgccatg ttcgacctgc ccgagcgaag
     9961 agagcctgac tgggcgaatt acagcacgct cgtgaagcga atcctcaatc ccagggaaac
    10021 cgttcggatt gccatggtcg gaaaatacgt cacaagcgga gacttctcgt taaccgacgc
    10081 gtacgtgagc gtgaatcagg ccctggaaca tgcaggtgcc gcaaatgaca caaaagtgca
    10141 gattgactgg gtaagcgggg cggatttcaa cgatagcaac gtttcagatg agcttcgtgc
    10201 gtacgatggg atactggttc ccggtgcgtt cggctcgtcc ggagcggagg gaatgatcgc
    10261 ggcgatccgt tttgcgcgcg agtcgggtat tccgtatctc ggactttgtt acggccttca
    10321 aatggcggtc gtggaatacg ctcgcaatgt gtgtggactc gcccgggcaa attccagaga
    10381 aatcgatccg aacactccgc acccggtgat cgacatcatg gatctgcaac gggcggtgat
    10441 cgaaccgact gtgtggcaag cctggcgggc catcagtcag cagatagaca tcgttgcgga
    10501 caactggctg aacagcaggc aacatcagat cggtggctca atgcgtcttg gcgcctacgc
    10561 cgcacgcttg atagaaggat ctctcgtgtt tcggctgtac caggaatccg gccgcctcga
    10621 gagcgataga tccaggatgg atcgcttgcg catgcaaccg ggacaggcgt tccgtctcgg
    10681 gagggaaaag cctggagctc ctgtcgtctt ggaacgccac aggcaccggt acgaggtatc
    10741 acctgcctac gtggaacggt tggagagagg ggggctggtt ttcagcgggt accacgaaca
    10801 ggcggacggc aaccgattga tggaattcct cgaactgcag gaacacccgt atttcgttgc
    10861 gacccaggcg catcctgagt ttcgttcgcg tctggaagaa ccggctccac tctttgtcgg
    10921 ttttgtcagg gctgcccttg agaggaaaca ttccgcagga aaatgaaaag ctcgccggtc
    10981 cttatcgtgg tgctgctggc gcaagcggtc gtgatggcag cctgtgacga catcggctcg
    11041 tcccgcagaa acgcgcctga ggcggctctt tcagggcaaa ggctggaacg atctcgtatc
    11101 gtgtggtcgg accgaggaag gaaaagcgcg gtagtttccg cgggagaact tatcagattc
    11161 gacaatcgca atgagattac tctgcgcgat tccgtggtcg tggaagtgta cgatgaaacg
    11221 ggtgccctcg ttgccattgc gcggggcgaa gagggcacgg tggacgaacg cagcgaaaca
    11281 gcccgcgtga caacaggcat ttctgttcgg ttctctggaa acgacgggaa ggaaacgtcc
    11341 accctcaccg cacggcaggc cgaggcagat aaccgcgcgc aaactgtctc agcgtcgggc
    11401 aacgttcggg ttcgcagtga tgggggcgta acggtgtata ccgaaaaagt gatctgggac
    11461 ggtcggacgc gcaggttccg tgcgcctggt tttgtccgcc tggtcaatgg aacagacgtc
    11521 gaggaggggg aaaacctcga tgcgaacagc gatttgaccc attggacaat ggacagagtg
    11581 cgcggccgaa gcactcgcgt gcgggaagaa ctggagagcg gctcctgaac atggcggcgc
    11641 ggacaacgac tctggcgacc ctgtttgtgg gtctggttgt ctgcggaacc tccggcgcac
    11701 aacagggtca gcgcgccgtt gaattcagcg ctgaccggac cgagatgata cgtcggggag
    11761 accgcttcct gaagcgttac accggtgcgg tggtcatccg cttcggaccc ggggaatggc
    11821 tgacggctga cgaggctacg tacgacagcg acggcaggaa cataaatatt gctggatcgg
    11881 tggtgatgca ggactccggg cgaattctgc gcgccgaccg ggttcaatac ctttttcggg
    11941 acaatccacg agcgggtggc cacccgttgg tattgttgct tgggagggtc gcgctggtcg
    12001 ataatgcccg tcggctaaac gctgaccgcg cggagtaccg ctctcacgcg gatagtctcg
    12061 ccgccttcgg cgatgtggtg gcgaaagaca gcagcaggac tgttaccgca gatacccttc
    12121 ttttgaaggc agatggaaac cggtttgttg caatggggag agtggtgctg gaagataccg
    12181 tcgaaaatgt aacggtccgc gcggggtact actccttcga tggggcggac agcgtcggtg
    12241 tgattgcgcg ggaaccgacg cttgttcgcg gcgctggcga tactgcggtg acggtgcgcg
    12301 ctgacagcat gatactgaac gttacgggaa aaaaaggaag cgcgtgggga accgtgcgga
    12361 tcgaaaaggg ccagatgacc gccgagtgcg attcgctggc gctggatgac aatcggggta
    12421 cgattgcgct tttcgggcac ccgcatgccg tacagaagcg gcaggaggac tccaccagta
    12481 cggttgacca tctgtgggga gaccgcatag tgcttgaggt ggagcagtca aagattacgc
    12541 gaatacttgt ggacggttcg gcgcgtacaa gcagcacccg tagtgactcc cgtggagggt
    12601 tggggcccga acgctgggcg accggttccc agatggtgtt ccacgtggaa cacgacgagg
    12661 ttcgtgaggt catcgtctgg ggacaggcca ggagccgtta cgaaccggat tttgaggacg
    12721 cgcgcgagag cggcacgaac gaggcagcag gcgacacgat acgcgtcgcc ttccgcgatg
    12781 ggaagcccgt tcgtgtgcac ttggcgggag gagtgcacgg tttgtacgtc cagaacgtcg
    12841 cacgtcgaac tgacgcaaca acacgcgctg cttccccgga cgaacgacgt ggtggatgat
    12901 tcggagattc gaagttccct tcgagcggaa gggctttcca aacgctacgg cggacggctc
    12961 gtagtcaacc acgtctcgct tgtggtgaaa aggggtgaaa cagttggcct gttgggccca
    13021 aacggtgccg ggaaaacaac gactttctac atgcttcttg ggatggtcgt tcccgaggag
    13081 gggcgtgtat accttgaaga acgggaaatc acaaagctcc cgatgtaccg gcgggcgcgt
    13141 ctggggatag gatatctcag ccaggaacgt tccgtctttc gaaagatgac ggtcgagcag
    13201 aacatcctcg cgatccttga acgcctcccg cttacgcggg aggaaagaat gcagcggctg
    13261 gaggaaatcc tggacgagct ggacatcgct cgtgtgcgga aaaccagagg cgagcaactg
    13321 tccggcggcg agtgcagacg caccgagata gcacgcgcgc tggtcatcga accgaagttt
    13381 cttctgctgg atgaaccgtt tgccggaata gaccctattg ccgtggagga catccaatcg
    13441 attgtcgcgc ggctgaaggc caagggattg ggtattttga ttaccgacca caatgtacat
    13501 ggcacactgg ccatcaccga tcatgcgtac gtgatggcgg aaggcggtat tctgtgtgcg
    13561 ggcacacccg ccgaactggc agacaacgag ttggcccgca agttttacct gggtgaacgt
    13621 ttccgtttga attgattcag tggtatcggt cgcaagggtt tcgccagtgc tgggaatgcg
    13681 tcaggaactc aggcttcaac agcggcttgc gccgcagttg attcaatccc tccacctcct
    13741 ccaattgccg gcgcttgatc tggaacaagt tgtcagggag gagcttgaaa cgaatccgct
    13801 cctggaggag gttccggctg gcgagttgga acacggaaaa gaagagcctg ccggggaaac
    13861 gggtaaggag gcggatgcga cctccgatat cgatctggac gcaactgagt ggtccgacta
    13921 cctcagcgac tcgtatgagc tgtcgcagac ctttcgcgaa gaacccgatc actctgcgga
    13981 gacgtacgag cccgtatcgg tgtaccagga gaccctggca gacgacctgc gcgaacagct
    14041 ccgcttcgtt ctctccgacg aaacagagct gaaaatcggc gagtacatta tcgggaacct
    14101 cgacagcaac ggttttctcg cgacaacggt tgaagagatt gcggccacgc tgggtgtgcc
    14161 ggccgcacag gtccagaagg ttttgaaggc ggttcaaacg ctggacccgc cgggtgtcgg
    14221 tgcgcgcgat cttcgggagt gccttctcat ccagttgcgg gagcgcggtt atgaagagga
    14281 tccggccttc gagatagtgg acaagtgctt cgacgaactg atgcacaagc ggacgaaaga
    14341 gatcatgcgc atcacgcgcc tgagcaagga agacgtagca gaggctctcg aagtaatatc
    14401 cacgttaaac ccgaagccgg cttccgtcgc ctacggttct tccgaacgcc cggtaacacc
    14461 agatctcatt gtcgagcgtg tggacgggga gtacgtggtt tcgctgaacg accgatccat
    14521 accggcactc aggatcagcc agaactatcg agagctcctt gcgggcggtg gtaccgaaga
    14581 aacgaagaaa tacatcgtgg acaagttgaa ttcggcgaaa tggctcatca aatccatcga
    14641 acaacgccgt acgaccatgc tcaaagtcat gaacgccatc gttaagcacc agcgagcgtt
    14701 tttcgactac gggcttgacc atctgaagcc catggttctg caggaaatcg ccgacgagat
    14761 caagatgcac gtttccacgg tgagccgcgt atcgaacggc aagtacgttc agacgccaca
    14821 tggtgtgttt gaactgaaat tcttttttga cggcaaactc ggtgtctctg atggcgaaga
    14881 tatggcctcg aaagtggtca aggaaaaagt gaagcaaatg atcgacgccg aggacaagca
    14941 gaacccgctc agcgaccagg cgattgccga ccggctgacc cgcgaaagtg ggatcgaaat
    15001 tgccaggaga accgtggcga aataccgtga ccaactccgc atcccatccg cacgcatgcg
    15061 gaaggaggtt tgatttgtcg ttcgacaggt tctggtcgcc cgttgttttg tcaatgctgt
    15121 tctccgggag tcttttcgcc agcacaccgt ttgtaacctc cactgaccgt tttcgaagtc
    15181 agctcgggat tattgagaac cccgctcttt ccgatgaagc gagcgcggtg tgggtgaacc
    15241 ccgcagggct tggtgtgcgc acatcagcga cgatgttttc ctcggttgca atgagaaaca
    15301 acccttggta cgccaaccta ctttttgccg gcaacggaag cggtttcggc tggcagcgaa
    15361 cggatgccgg cagcggccaa cgaattgacc gctggcgctt cggaggaagc ggcggaagct
    15421 ccccgtacgg cctttcattc ggggctgcgg tcgaactgag tgatccggac ggtttgaacg
    15481 agaatctttt ctggtcaggg gaccttggtg ttctcgctag gccggttacg tggatgtccg
    15541 ccggccttgt cgtgcggcaa ctcggcgctc gccgtggtta tccctggagc gtggaatcgg
    15601 gccttgctct gcgcccgttc ggaccgaacc tgagcatttt tggcggtctc gcgtactgcg
    15661 aagacgaccc attgaatgat ccttcacact ggcatgccgg tgcgctggcg aacgtggggc
    15721 ccggtcttga ggcgtacggg gcgatcaatc aagacaggac gattctggtc ggcgtccaga
    15781 tgattctcgg aagaggcagc atcggcggag cagggagtcg cgtgagtggc ggaagcgtcg
    15841 gttcaggctg ggtgattgct cgctcccatg cggattaccg gtctaaccgg ctggcgatga
    15901 aaggcaggat cgcagagatt cggctgaaag gtgagattcg cgatcagacg ccgggattca
    15961 gcctgtttgg caaccggggc accacgcttt cggaactcgt catgcagatc aatcgggcgg
    16021 cgcaggcgcg tgatgtcggt gggctgtacc ttcgttttga caacctggcg atcgggcagg
    16081 gaatggcgga ggaattgcgt gatgcgttga tcaagttcaa ggcgaactca ggcaagccga
    16141 ttgttgctta tctgccggag gcgagtttcc gggaatactt cattgcctcc gttgcggaca
    16201 gcatcttcct ggagccggtg ggcgacctcc gtctgaccgg gtacggcgtg gggcaattgt
    16261 acttccgcag ggctttggac aaactcggcg tcgaagctga tttcacccgg ataggccgtt
    16321 acaagtcggc cgctgaaacg tttacggaca gcaccatgtc ggatgccacc cgggagcagt
    16381 acgaggaact cctcgacgat tggtacacga gaacggtaga tggaatcgcg gtgtctcggc
    16441 gcctcagcgc tgacagcgtg aaggctctgg tcaataacgc tccctacatg gctgcagaag
    16501 cagttcgtgt gggcctgatc gattccgcag gtcacagtga tcgagcctat gaatccgtgg
    16561 aaaccatggt aaggtcgaga gaggggagag tatcgggcaa aataaacctt gcgcgtcgtc
    16621 gtttatatga tgagacgtgg ggcccgcgtc cgaaactggc ggttatcttc gcgtccgggc
    16681 agattgtgaa tggaacgagc ggggaggatt tcttttcggg aacccagatg atgggtgccg
    16741 agacaattgc gaaggcgctc aaacaggctc gggaggacga tgccattaag gcggtcgtgt
    16801 tccggatcga ttcccccggg ggactggctc tgggcagcga catcatatgg cgggaggtac
    16861 agcttttgtg ggaaaccgac aagcctgtcg tcgtaagtgt gggcgatctg gctgcttcgg
    16921 gcgggtatta catcgcgtgt cgcgcagaca cgatcatttc gaatccgggc gccatcgttg
    16981 gcagcatagg ggtatttgat ggcaaaatgg ttgttgagag actggcgcgc cgactcggaa
    17041 tcgatgtgga acttctcgcg aggggagaca acgcggcgat caattcttca ctcgcatcgc
    17101 gaacaccgga gcagcgaagg cgagtggccg aaaacgttcg tgaagtctac gacgtgtttg
    17161 tgaacagggt cgccacgggc cgcgggatgg aagccgcctc ggtggattcc atcggtcagg
    17221 gccggattta cacggcagcg aatgccgtca gcataggcct cgtcgacaaa ctgggtggtc
    17281 tggatgaggc tatccgaacg gccgcccgga tggcccgcct gcgtggcgaa gtcgaactcg
    17341 taacaatgcc gaggcgcaca aatgtgctgg aaacggtgat ccagagctcg ttgcaggatg
    17401 ccatgggtgt gtcgacccgg cagtccctgg ccggagaggt ttacttcttc gacccggttg
    17461 ccgcgtcgtt gcgatgacac gggtggtttt cggatccggg gttttgaata ttgggggccg
    17521 tttctggggt tacacggttg tacggatgtg tagaatgagg gatgttgtga cgggtaccac
    17581 cgggtttgca gcgccagcag cgaaatcgct gctggctggc gcgatcgttt tgtgcctgat
    17641 gtgctcccac gcgcatgctc aggtgggtga attcggccgc aacaagccca catccgccac
    17701 gggaaactgg cactatgtcc agacgccgca tttcgacatc tacttttacg agggcatgga
    17761 cgcgatcgcg agaatcgcca cgacgatagc cgaagaggcg tattcccaga tcagtgcgag
    17821 cttcgactac cgcctgcgga accgcgtgcc gatcgtgttg taccggtccc aggggcagtt
    17881 ttcgcagacg cacatcacgc cctcactgat cggtgagggt gttggcgggt tcacggaatt
    17941 tctcaagaac cgcgttgccg tgccattcac cggatcctac ccgcagtttc gtcatgtcct
    18001 ccaccacgag ctcgtacacg cgatagtgca cgacatgctt tacgggcgag acattctgac
    18061 cggcgtacaa cagagtgata tccctctctg gttcatcgaa ggtcttgcag agtacgagtc
    18121 gtcgccgtgg acttcagaga gcgacatgtg gatgcgggat gcggtgatca acgattacct
    18181 gcagctcgac ggttacttcg cctacaaagg cggccagtcg atcatgtatt acattgctga
    18241 ggtgtacggg ccggacaagg tggcgcagat cctgaaccgg cttcgctctt tttctcgctt
    18301 cggtccgacg gttcaagccg ttctgggggt cgagctcgag gatttcggaa aaaggtggga
    18361 gaaagacctc aagaaggtgt actggccgga aatcgtcgat cgagacgagc cagaggatat
    18421 cggccaatcg ctgacagacc acaccaaaga caactcctac ttcaatcgga acccctcatt
    18481 ctcgccgttc ggcgacaaga tcgcgtattt gacgggcagg gattactaca ccagcatcta
    18541 cgtgatgtcg gccatcgacg gaaaggtgct ctggaaagtc gttacgggtg aaacttccgg
    18601 gtcctttgag gaaatgcact ggcaacgggc aggaattgcg tggtctccag atgcgcaacg
    18661 gatagcgttt gcggcaaagg ctacgtcggg cgacaggctt tacatcgtcg atgcgcgcaa
    18721 cggacggagg ctcgacgcgt tcgatttcgg attcgatgga atgttttcgc ccgactggtc
    18781 accggacgga actacgattg tatttgttgg cgtgcgtgat gggaaatcgg acctctacac
    18841 acttcggctt tcggaccggt cgattgaccg gctgacggac gactactttg atgagactga
    18901 tccaaaatgg tcgcccgacg gccggcgaat cctgtttgct tccgatcgag cgcccgatgt
    18961 gagcgatgtt tcagcccagt tgctgtacca gacatcggat gtgtacgtga tggacgtcga
    19021 gacgcgcgca atgcgtcgct tgacggcgga ccccgcggac gatctttcgc cggcatgggg
    19081 gcccgaaggg aaacacatcg cgttcgtgtc cgaccgcaac ggtattttca acctgtacgt
    19141 tgccgcgctc gacagcggcg aaggaagtat cgacagcgca acacgtcctt tgacaaacat
    19201 cctcacggga gcgttcgctc cgtcctggaa tcgcagaggc gacaagatcg ccttctccgg
    19261 tttcaataag ggggggtggg acgtttttgt catcaggaat ccactggaca gccttcgtga
    19321 ggaacccctt cggccggcat atttcctgcg cgaagggaag atttcgtaca gcagccgcca
    19381 ccgccacctg gcgacgagca cgcagtccgc cacgtcacct gattccacgg ccggattctc
    19441 gatagggcct cgcaccagcc cattcggttc ccccgccctc gatatccttg ctttcacccg
    19501 ggcagattcc gcgcgtggcg gcttcgtgcc aaagccctat cgaagccagc tccaggtaga
    19561 caacgtaagc ggacaggcga attactcgac tctcggtgga gtcggtgggc agctctacgt
    19621 cacggcgagc gatctcatgg ggtttcacca gctccagttc aacaccagtc tttaccggga
    19681 cctcagggac agcgattacg gtgtgtttta tcagtatctg aaaaggcgaa cagactacgc
    19741 cgcgtatttc cagcaacaac ggacatggta tcctttccag agcgtggatt attacggcgg
    19801 gatcgtggaa tccgggctgg tagaaagccg ggagatttca accggcgttt atgcgtcccg
    19861 cccgacgagc cagttccgcc ggttcgaagc gggcatagac tatgtgaacg cgaaacagag
    19921 tgtgctttac gactacctca gctacggcac cggcattgcg gcgtctcagg aagcaaccat
    19981 tgaggctttg cttacaaggg ttgcgttcgt gcacgacaac gcgctatgga acatgtttgg
    20041 tccggtggta gggcagcgct ggcgtatgac ggtttttgct gcgccgccgc gcgccaacac
    20101 cgctccattc ggcgtagtga ctgcggatgt gaggcgctac tggcgcgtaa gcaagaatac
    20161 ttcattcgcg ctgcggggct cagggggacg gagttttgcg acgcatgcac aaaatgcgcc
    20221 tctgttctat gttggcggtt tgccgtactg gatcaactat gattacgaga acgtgcaatc
    20281 cagccaactg gtgaacgacg cgttttcgca gtttgtattc ccgctgcggg gcactaatta
    20341 ctacgagttg agaggccgaa gcttcgcgat tctgaatgcg gaataccgat ttcccctgat
    20401 atatttcctt gcgacgggcc cgctcaactt tgtgctcagc aacgtcggcg gcgtcgtgtt
    20461 tctggatgtc ggccggacat gggaggaccc gacgcaaccc agcgacccga acatagcccg
    20521 tcatgcgcag ggtcccctcg ggggatacgg ctacgggatg cggatcaacc tcggtgtgct
    20581 ggttctgaga atggacgtag cgtggcgaac agacctgagg gaaacgatcg gcagcgcgag
    20641 gtattactgg tctttcggct acgatttctg acacaccggt ccgccgagag caatcaggtg
    20701 gaacagcatc gcgacgccga tgtgaacctt tccctgtgat tccgcaagag gcatctccgt
    20761 ggaagacagc gctcgaacgc ctgcgcactt tctttccccc gatctcccct ttgtgacaaa
    20821 ccgccatgca gaggtgttgc ggcgtgtccg cgtcgtgctg gttggaccga aataccctgg
    20881 caacattggg tcggtggcac gcgcgatgac ggtgtgcggg cttacccgaa tgcacctcgt
    20941 caaccctgtt ccaagctggc gggagtcgga tgaggcctgg actttcgcct gcgggtcgcg
    21001 ttccgttctg gagcaggcga ccgaagcgag gaacctggaa gaagcccttt gcggagcgac
    21061 gaccgtgatt ggtacgacgc accggatggg aagaggccgg ggcccgctcc tgcctccgcg
    21121 aaaactggcg gaacatttgc cggagatgct ctccaaaggc gaggtggccc ttgtgttcgg
    21181 acgggaagac aaagggcttt cgaatgacga actggcgtta tgccagatgg gtgttcacat
    21241 accgacggcc tctacctatc cgtccttgaa cctttcccac gcggtaatgg ttgttgcgta
    21301 cgagtgtttc tacgcggtag tgacgacggg ggatgagaac gtgcacgtga gcggggtgtc
    21361 gtacccggaa atcgtggctc tttcggaaag aatcgcacat ctgaccgcgc ggactggctt
    21421 tcgtcaccgg cggggagaag tgggctttga acgcagcctg cggcgagcca tagaacggag
    21481 tagtctgagt gcccgcgaca ttgctctcgc gcatctcgtg tgcagacaag tcgagcagta
    21541 tatcgaccgc ctggaggagc agattcgcaa cgagcgggaa acgtcgaact gatcgacgac
    21601 aatggcaaaa atgaaacgcg gcgaggacag cggaagtcgc tccgcgtcct cgccgcgtgc
    21661 gccaaactca gacactacag ggcagcgttc cccgattcgc cggtgcggat cctgattgcg
    21721 tcatctacag ttgagacgaa tatctttccg tcgccaacct ttcccgtacg cgccacccgc
    21781 aagagcgcac ttaccaccgc gtcaatctgg tcatcaagga ccaccgcttc aatcttgact
    21841 ttgttcacga acagaacctg atactccgca ccgcgataga actcggtgtg cccacgttga
    21901 tggccaaaac cacgagcctc gagaaccgac aggcccttta caccggcatc tgtcagcgcg
    21961 gtgcggacgt cctcgaccag atggggacgg atgaatgcct cgactttctt cacgatctgt
    22021 ctttcccgtt ttgttcctgt ttgccgtgcg cattacaggt gataggcggt ttccccgtgc
    22081 tgtgtcaaat cgaggccctc acgctcttcc gcctgggaaa tacgcacgcc gccggcaatg
    22141 tctatcagtt tcaagataat ccatgtcgcc gccgctgcat aggcagcagt cgccaccacg
    22201 ctcagcgcct ggaccagaag cggatcgacg ttgcccagca gcaagccgtc tttgccgttt
    22261 gcattccagt acgtgctggc gaagactccg gtcagcaacg cgccaagggt accaccgaca
    22321 ccgtgcacgc caaacacatc gagggagtcg tcgtacccga atcgtggttt gaggcagatg
    22381 gcccagtaac agatcacacc cgccgcaagc ccgattgcga gcgcggccat cggtcctaca
    22441 aacccggatg cgggtgtcac cgccacgagc ccggcaacca tcccggaagc gactcccagc
    22501 acggttacct tccctgtccg aattcgttcg gcgaacgccc aggccattgc cgcggcggcc
    22561 gcagcgatct gcgtggtgac aaacgccaat gtggcaagcg cgccgctggc gagagcactg
    22621 cccccgttga aaccaaacca tccgaaccag agcataccgg ctcccagcac cgtccagatg
    22681 aggctgttgg gaagtatctg gtctttcggg tatccaactc ttctgccgat gtaaatggcc
    22741 gccacaagcg cagagaagcc cgaactcatg tggacaaccg tccctccggc gaagtcgaga
    22801 gcacctaact tgaacaacca cccgtccgat gcccacaccc agtggcagac cggtgcgtac
    22861 acgacggttg tccagaggag aacgaacgca acgtatgcgc gaaatttcat gcgttccgca
    22921 aaagcaccgg tgataagggc gggggtgatt atggcgaaca tcccctggaa cgcgacgaac
    22981 gcgaggtgag gtatgcttcc catcggctcc actccgaccc cgttgaggaa ggcgtggctg
    23041 aatccaccga tgaacgagtt gccctttgag aacgatagcg agtaacctat caccacccag
    23101 tggatcccca cgagtcccat tgcaaacacg ctgtgcatga tcgttccgag cacatttttg
    23161 ctgcgcacca ttccgccgta gaacaacgcc agaccgggcg tcatgaacag aacgagagct
    23221 gtggacgtga gcatccaggc agtgtcgccg gagtcgatac tcccggaacc ctccgcaaat
    23281 gcgggaggga cgatcccgag gagtagtgta aggaatgcca tgagccgcct gttcatccga
    23341 acctccaaat cagaaacaga acctcgccga acctctgctg cgtggggcat ttcacctgtg
    23401 cctcccgctc ccttattgac gatagccacg gcgtgtttcc gtgaaatcac agcggcgttt
    23461 cgcgagtgta aaaaaacgat gaagttttgc agacatggtg ggtgttgcag gtgagacgga
    23521 gatagtggcg ggagcaggag ttacgcgata ccgtcaagaa tcaatgacgc gaacacggaa
    23581 taccagtaat cgggaacaaa gggcgcatga agcaacgcat ccagcgtata aagggtcagt
    23641 tccagccggt cttccctgcg gaattccgtc gtgcctgtgt acccttccca gaggaacatc
    23701 cagtcgggat cgttcacgaa agttcgtttc gtgtagtacg cgcagctcat ggccccgtat
    23761 aacggcgcga ggtcgtaccg ggcagggccc acactgagcg tttcgaagtc caacaggccc
    23821 gataacgcac cggtgaaccg gtcgaaaagc atgttggcga tgttcgcgtc gccatgaatg
    23881 gggaccagcg tgttttcgtc gaacctgtgc aggttctggt cgaggaaaaa gatgagccga
    23941 gcccgcaacg tctgtgacac gaggccgctt tccgccagtt ggactgcgcg tgcttcaaac
    24001 gattgagcga acctttcctt ggaaacgagg tggtcgaaaa agggagtgaa aagttcctct
    24061 ggcgcgaggg ttttcgcctt gtgccattcc gccatggcct taccgaccga acgcaaagcc
    24121 accggcagca gcgagggagt ggagttcaac atcagatcgc cggtaactcc cgggatttgc
    24181 ttcacgcagc acaccccgca gttccatggc tcttcgggtg gcagtacaat cacttcttcc
    24241 gcgcagggga ttcccgcgcg acgagctcga cgccatccga cgtattcgcg ccaccatccc
    24301 tgtccgtccg atgtcgggct gtcgtcaaat ttgatggccc aacgttcgcc ggattcgaga
    24361 accagcatcc gcgaacctgt gcgatttgcg ggcgcagtga gcgggtggga agaaacgacc
    24421 ggcgccagcg acaaacgacg cattatcgcg ttcaacgtat cgtgatcaac aagccgaagc
    24481 attacgcctg cccgttcatg cccatcgttt ttgcgcgcgt tacgagcgca tccagcagat
    24541 ccaggaagga tgccgcgcct tccacgatcc tgaagcggat tgagagtacc catatcgggc
    24601 atccgcaatc gatatgcgaa gggagacgaa ccgcatcctt ttccgtcgtg atgatggctt
    24661 cgcagcggag ctggcgtgcc gcgtcggcga ttcgcgacaa cgcacggtgt ctgtatttcg
    24721 catggttggg aaacctgagg tgatcaagca cttctgctcc gagcgttcgt acagactcgg
    24781 caaatgacca gggatttccg atacttgaca cggccaacac gcgtgcgttt ctaagaattg
    24841 tcagatcaag cgaatcgccc gtggggtgca ccagcagccc gtcgggggcg tggacaccca
    24901 ggacgacagg agccgatggg ttcaggcttc gcagacgctc ccgcaattca gacaacctgt
    24961 ctgtctggtc tgaacgcgac acgacgatta catccgcgcg cgccagattt gggggcggtt
    25021 cccgcagggt gcccccggga agcgttttct cgttaccaaa cgggttgacg gcatccacca
    25081 gcacgatatc aaggttcctg tccagtttca tgtgctggaa tgcatcgtcc atgatcacca
    25141 catcgggagc gaacctgctg accgcaacaa agcccgcgag agctctgttt ggactcacaa
    25201 ttaccggcac gccgggcaga gttcgggcga gaaggtacgg ttcgtcccct gaccttcggg
    25261 gtgaaagcaa aaggcgtttg ccgtccgaca caacggccag tgtggcgcct tctttacggt
    25321 agccgcggct tacgatcaca ggtctgcgcc ccgtgtccag gaaatgccgg gcaatgaact
    25381 gggcgagagg tgtttttccg gtgccgccca ccgtgatgtt gcctatggat atgaccggcg
    25441 tgttcagagt agtttttcgg aacacgccac ttcggtaagc ccaggaacga gccctcactc
    25501 cggcggtgta caccgttgaa gccgcagcca atccgttcat caaggacttc cccagcttgg
    25561 ttcggggcgg gttcagaagc cattcatcga tccgtctcac cttggcacct ctgtcaatcg
    25621 tgtgctttgg acctgctcgg gagtcgccat ctggccatcg cctctacggt cgctgccagc
    25681 gcgtccggaa ttacgctctg gaaaggtcca gggtagttgc ggaggatgtc cgcaatgatt
    25741 gcgcccgcca tcctgccatc atgagcccgc gtggcctgac cacttgcctc gagcagattg
    25801 catcccgcct gttccatgtg cggcccgtac acaactggca ggccatacgc cgtcgcttca
    25861 aacgggttgt gtccgcccaa aggaacccat gccccaccta taacggccac atcagccccg
    25921 gcataaagct gcgcgaggac tcccattttg tcaacgagaa gcacggattc cggtgtcaaa
    25981 atccccgtgt gagcgaggct cgacagccga agcggacgca agcctccacg tttcaggatg
    26041 gtatctgcat aatcgtaatc agtcatatgg cggggcgcaa cgacaaaacg ggacgaaaca
    26101 cccgcgaggc gaatctgttc gatggcagac acaacgtgag acatttcttc gcggtgcacc
    26161 gagccgaaca caactacccg gtcgtctcca tccatctgca ttgccgcacg ggcatccgca
    26221 cggtttgcca gacttccgcg gcaccatttg agcgatccgc acacggcggt ctgctcgcag
    26281 ggaactccca gcgaggaaaa ccgtgcagca tcgtctcctg tctgcgcaca gacaaacaac
    26341 gcgggcgcga cgtccgtcag aaggcggctt gctttcatga gcgtcgtggt tcgttgagaa
    26401 acccgtgcgc tcaccagtaa gacgggtacc cggcgttgca ccattgcgtg gaacaagccg
    26461 ggccatagct ccgtttcgct cacgattacc agcgcggggt actgttttct gcacattgat
    26521 gcccacgacg ctgccgaatc agcggggaac aagcggatgt gcgtacactc gattccaaat
    26581 tgtgaccaca tggccacccc tgtgcgcgta catgagagat gtacgtcaac actggccaca
    26641 ccgccgaagt gctcccagag cggcctcaat ccgcggacct ctcccgcaga agcaccgtgc
    26701 cacatccaga aagggaaggg gacgtccaaa ggtttcggaa acaaacggtc tcgcacttcg
    26761 tatctgccgg tcgcccaagc gagcagaagg aggggactcc ccgccgacaa accggatttc
    26821 cacagcaaat cgtagcaaaa tgcccgcgct gacattcgaa ccacctgcat gacgggagaa
    26881 ggcatgagaa atatacgttc ggcggacgac cgacgtcctc actggcacgc ggttcagcgt
    26941 gttgttgctt gttcagtcgc cgtcgacgcc gtacttttga agaagaagac gaggggccca
    27001 gaccgtctcg cgtcgttgga atcatctcga aagtcggacg cggcggacca cgcagtctgg
    27061 cgaaaattcc cctgttgtgg cgtagagcct tcgggacgag attcgggaaa aacgtcgcat
    27121 gcgcggattg tctggtgacc cgccaaaggc gtcctgtgtg tctcggcgta atctgacgcg
    27181 atgtcgcgca cacacgctgg gccgggagac catactgtga gtggaacgat tcgggttgcg
    27241 attgtcggat gcgggggaat cacgtccgcg catttgaacg gtatcaggat tctccagcaa
    27301 tcagggctcg atgacgttca agtcaccctt ctgtgttcgc gcgatctcga aaacgccgcc
    27361 cggtataaca agcggggaga tgcgccgccg ccccttccgc ccatcgtgcc gtggcgcgat
    27421 gatccgctga acattcgcga tgtgtatgtc actgaccttc atccgggggt gccggcggaa
    27481 ctttgcactg attggaggaa agcggtcgtg tcgcccgacg tcgacgcggt tatcatcctc
    27541 gcgtccgttg gagtccacca cacaatcgga tgcgccgccc ttggggccgg gaagcacgtt
    27601 ctcattgaga agccttttgc catcacggtc cgcgcggcac gaaaactttg cgaaatcgca
    27661 cacgaacggg gcttgtcgct gggcgttgcg gagagtcttc ggtacaaaag ggagacaaga
    27721 tccgcccggt gggccctgga taacgggtac atcggaaaac ttgatatggt ggcgcacgtg
    27781 agcctgggat cggtgtggtc acctgacaaa atcgtcggca gaaccccgtg gcggcaccac
    27841 aagcttgaaa ccggaacggg tatcctcatg gatatcggtt cgcacttgtt cgacaaggtg
    27901 aggtacgtct gcggtgagtt tgacaccatc tcggggatca cgcgcacgct cgaacctttc
    27961 cggacaacaa gggacgacgc tggtcacata atcgcgcaaa ctcattgcga cgtcgaggac
    28021 acggctttcg caacggctgt gttcaagaat ggagccattg gaacgttcag catgtcatgg
    28081 gccacacacg gagaacctgc gtcgcttccg ggtggaaccg cgctgttcgg cgacctcgga
    28141 tgcatcaagg aaggaaaact gatcggcgaa cgcgaaggag ttcgggttct ggaggatctt
    28201 ttcgttcggc gtgccccgaa aggactccag gaagaatggt tcccacgagg cgttacggat
    28261 gcgtttgcgc tggaactcca cgaattcttt cggtccatac gggagggtcg ttctccggag
    28321 acgagtggga ttgaagggtt gcgcgatgtt gccgcatgtt acagcgtcgt ggagtcctcg
    28381 gtccgcggcg aatgtgttcg ctttgaggat gtggcgaact gccgggtgga gacctatcag
    28441 agggctatca acgaccaact gggactgggg tgaacgttgt tgggaccttc ggctgtttgt
    28501 ttggtgaggc acggtcagac tgagtggaat gtcgagcgga agtggcaagg cgttacggat
    28561 atccctctca atgctcgagg tcgtgcgcag gctgcggcag tggcgcgcat gttctcctcg
    28621 tccgggatgc ttggcggcgt agcgatcagg cgagttgtgt cgagcgattt gagccgtgcg
    28681 aaggaaaccg cacacgagat cgctcgcgtc gcacttgttc cactggagac gaccccgcgc
    28741 ctccgggaac gttccttcgg gcgctgggaa gggctttcga ggagtgagat tgcccagcag
    28801 tttccgggag atatcgaggc gtatgaaaca caacgggaaa catttcgtcc gcttggtggt
    28861 caatcatggc aggagttttc agaacaggcc gcaagaacgg tgcacgaact tgtgggcgat
    28921 tttgccggtc aatcgaccgt ggttgttgcg cacgccggtc ctttgcaggc gttcctcagc
    28981 caggtgatgg gcacccgaga ctcccggccg ggcaggttca tgatagacaa cggttctgtg
    29041 agcgttgttc gcgagtggct taacgagcac actggtgatc gccactgggt ggtgacggcg
    29101 ttgaacataa cgtgtcacct gcacgcgttg gaatcctgaa aggccccgtt gagatgcccg
    29161 gtttgtttat ttcatttgag gggatcgatg gctgcggaaa aacgacgcag gttgaacgcg
    29221 ttgccgagat cattcggggc acagggaggg aagtggtttc ggtgcgtgat ccgggctcga
    29281 cgcgcgttgc cgaactggtt cggaacatgc ttctcgaccg cggcaattct gatattgacg
    29341 cacgcactga gcttttcctg tatctcgcat gtcgagcgga gttggtggag cggtgcattc
    29401 gtcccgccct tggccgggga caggtagtcg tttcggaccg tttctccgac tcaacgctgg
    29461 cgtaccaagg gtacggccgg atgctgggtg tggaacttgt gcgccaggcc aatcgcattg
    29521 cgaccgccgg attgacccca aacgtgacgc ttttgattga cattcccgtc aacctcgcgc
    29581 gaagccgacg gggtgcaaag atcgtggacc gcctcgaggc tgaagacggg gatttccacg
    29641 aacgtgtcag gaacggcttt ctggaaatcg cacggcgaga accggatcgg gtagcggttc
    29701 ttgatggaac cttgcccgtt gacgatgtta cgaacgcgat cgtggatacc cttgcgctgc
    29761 gagtaccagt tttgctggga gatgtgatca agagttcgtg agtgcgtcgg tttcctcgtc
    29821 gaaaactgcg cggtatcggg cgacgatgtt gcgttggaac aaaccttatg aaaccggagt
    29881 ggaggtagtt tatggcactg cgccgccgtg actggggctg gctggccctc atggttgcag
    29941 tcgttatctg gggtggccag atattcaacc acgtcgaggc acttgcccag gatgcgtacg
    30001 ggagaattgg ccagatggcc gatgaccgtg cagaggtgga aaccgcaatt ctccggtttt
    30061 acgtcgagga cgtagacgca gggaagttgc agctctccca ggttgacggg atgctcaacg
    30121 aactcgacac gtacagcgat tttctcaacg agcgcgagca agaagacctg cgcatgacta
    30181 cggaaggggc cttcggcggg cttggcatag agatcaacgc ggtcgatcat taccccacgg
    30241 tcatttcgcc ccttgaagga acgcccgcgt gggacgccgg tctgcttgcc ggggaccaga
    30301 ttatcgaaat cgaaaatgag agcacgtatg acgtgaagct tgatattatc gtcggcaagc
    30361 ttcggggggt gcctggaacg gcagtaaaca tcaaggtgcg ccgacctgga tccagcgagc
    30421 ccatcgactt ccgtatcaca cgtgctgaga ttcatgtcaa ctcggtccag ttccatggcc
    30481 tgctgtggga tccccgtgct gaagggttcc tgccgcttgg tgctcctgac tggttgcgga
    30541 acgctcaagg catcggttac atccgcctgt cgtcgttctc ccgcggggcg acggacgaag
    30601 tccgcgttgc gttgcaggac ctgatggcga agcgtgcccg gggtatcgtt cttgatctgc
    30661 gaatgaatcc gggaggcctc ctggaagagg cacggggagt cgctgacctt ttccttccga
    30721 aaggtcgcct gatcgtctcc acgaaaggcc gggcccagag gggtgaacag agactgtatt
    30781 cggagcgcga tccggccatt cctccgtcga tgccggtggt ggtactggtg gacgagggct
    30841 cagccagcgc ttcggagatc gtcgctgcgg cgattcagga caacgatcgg gggttgattg
    30901 tcgggcgtcc gacctttggc aaaggatccg tccagacggt gtacaactct cagttcagcg
    30961 ggagattcgg tctcagtttt tcccagaacg cccttctgaa acttacgacg gcctactact
    31021 actcccccag cgggcgctgc attcacaagc cacgctggcg aacgggcaag cgtggcgcgg
    31081 ttgtttcgaa agctgcgagt gataccgcga cggtgtaccg tacctcgaag gggagagagg
    31141 ttcggggtgg tggaggtgtg gcagtggacg tggcggtgca accggatgtt ccaccggcgc
    31201 tgttctggca actcaacgcg cggcagttgt ttttcaacta cgccgttgat tacgcggtga
    31261 aacacccgga actggatccg cagacattcg acgtcaacga agcgatgttg tcagatttcc
    31321 gcaggttcgt ggcggactca gtacacgcgt tcacgtacga accacggggc aaaaaggagc
    31381 tcgatgactt cgcgaaggcg acctcggagg ccggatacag tcaggaagtt acgcgtcgtg
    31441 tcgaggaatt acgggaagct ctcctggcgc agaaagacgc ggagttttcc cgtgcggagc
    31501 cgtacattcg gcagcggttg cgcagtgcca tagcagggcg ggtctgggga aggcaatccc
    31561 gcactcgagc agatttcacg cgcgatctcg cgctcaagga ggctctgaca tacctgacgg
    31621 attccggccg gtacgcacaa cagattgcgc tgagcccggt acgcccgcgg cctcaacagc
    31681 aggaacagac agccgaaaac gaaggtgatt gagacgcgtg ttgcgtcttt cgacttacgg
    31741 gttgggtgtg ggagcgctca acccgtttgt cgtttcccgg agatacgaac tcgcctttct
    31801 gtttcgttga tagaacaaag aaagtggatt gcagtggaac tggcaaatca gagcgattcc
    31861 gaacttatca gcatgttcct tgatgggcag gctgccgctt ttgatacgct ggttcgtcgc
    31921 tacgagcggc aggttttcgg gttcattttc ggaatggtgc agaatgaggc gctggcgaac
    31981 gaccttttcc aggaaacgtt cattcgcatt atcaacgcgc tgccggacta ccgggaacgg
    32041 ggaaaattcg cgagctggtt gctgggcatt gcgcgaaatc tcgctctgga tgccctgcgg
    32101 aagcgtcgcg ttgaaggccg gattttccgg ctggagaatc gcacaacgga tagcggaaac
    32161 gcggagtcga catacccgga gattcccgac gacatagaaa gccaggccga tatggtggaa
    32221 cgggaggaat ggctggttcg catgagggaa gccctggctg aattacccgt ggaacaacgc
    32281 gaggttgtct acctgcgcta cgaagcagat ctcaccttcc agcagattgc ggacctgacg
    32341 ggagtctcca tcaacaccgc tctcggccga atgcggtacg ctttgaacag cttgaggaag
    32401 cgaatgaacg ttgccacgga gccttcaacc aatgaatgac acaaaaggga caccggcgtg
    32461 tcttcgcacg caacgcgacg ccctgcggta cacgtcaggc gaactctcgg ctgaggagcg
    32521 catcacgttc gaggtgcatc tacaacactg ccgagaatgt gcacggttgg tggatgacga
    32581 aagaacaatc cgcagagtgt ttggagcgct tccgcgatcc tctccatcgg ctacggtccg
    32641 ggcgttgata tcggaacggg ctgccgcgtc aggcgctgcc taccacaaac tcagcagtcc
    32701 ggggcggatt gcgccgctcc gcactgcgct ggtaacccgg atccccgtgt ggacctggcc
    32761 ggttgcggct gccgcgattc ttttgctcgg ggtctcgttg accttccaca gagatgtgcg
    32821 aacgccggta tttccgagtg acaccctgtc cgcgcctgtc gcgctggtac ccgatgtccc
    32881 ggcaggagac ggtatccagg ttgcctccgt ttctgaaacg acaaaggctt caacgcggat
    32941 aagaactccg agggacgggg caagcgcatc cgacaatttg cttgatgatg atgttgtgga
    33001 gcttgcggac cgcgttgaga cgctcgcaat gattctggac gaggaaacca ctcaatgaaa
    33061 tggtcaagga cgttgtctct tatgtgttcg gtcgttgtgg ccggggtggc ttttctgaac
    33121 ggaatcagag ccgaagctca gatgcccggg cctccgccgg gtggtttccc ggaattctca
    33181 cgtggcccca ggcggggcca actgaggaac cccaccccgg aggaagaggc aatggcgttg
    33241 gagtttatcc gaaggataga ctcgtcgcgc gtggaacagg tgatcgccct gaaaacttcc
    33301 cgtccacacg cctaccgctc gatgattctg cgctctctga acatggaacg gcatgcccgc
    33361 gcgatgggga gaagaggcga tcccggtgaa atccggcaat cggagcagtt gttccgaagg
    33421 atgtttgaac tgcgcgaaca gatggacagg ctgtcgatcc agtaccggct ggcaacggac
    33481 gaaaacgcga agaacgagat tcgggcgcgg ttttcggcaa aggcaggtga acttttcgat
    33541 gttcagacgg agatgaaccg cctacagatt gaacagatag cggacgaact gcgccgcctg
    33601 caacaacgtc tcgaacaaag acaggcgggc cgcgacgcac acatacgccg ccaggtggaa
    33661 atcatgcttt ccgatgagga gcttgactga acccatcgtc cggttctcac caccggattc
    33721 tgcgcgccaa atcctccccg agcatcctgc ggagatcttc acgagccctt gcggggtgtg
    33781 tccccttcca gaacaaacga ccacacaaag ggcactcggt gaagatttcc tgtgtgtcgt
    33841 agacatactc ccagacgcgg ccttttgcgg actcttttgg aaccaccttc accggaacgt
    33901 tgcagcgtac gcaacgactg aatggcacca acgccgtcag gtcgaagtgt gcggccacct
    33961 gtgcaagctg gtccggcaaa cggtcgaagg tcacgccgaa acgaggggtt gagccgggca
    34021 ggccgagcaa ccccctgtcc cgggttaata cgagcctgtt ttcgttgcga gcccgttcca
    34081 tgaggtccgc gtcggaaatc catgactggt atgagacatc ggcgccgagt aacctcaacc
    34141 aggtggcaag gcgcccgagc atcatatctg cagcgcaagg cggtagaggg ggcaaatgta
    34201 gtgcttcagt catgattgtc tctcacactg cggttggaac ccgcaacagg ctcgaggtac
    34261 ccttttgttg cacatgtcgc tctgcggcct gtcgggacgg ttgccaattc gagcacttcc
    34321 tctgtttttg ttcaccttga gacttgactg atgaaagaat gggaccggcg gatcgcgcac
    34381 ccgattacag ggcaagaaaa cccggcatac ctgcacgggg tgattgttcc gatgtttacg
    34441 ccctgcaact cgaacgggac tctggacgaa cggggtatcc gttcctatgt gaattacctg
    34501 gtttcaaacg gaagcgtgag cgcgctgttt gcgcgaagtg gactcggccg catgtacacg
    34561 ttctccgtga atgacgtccg gcgtcttgcg gacattgtga ttgacgaagt tgccgatcga
    34621 ctgccggtgt tcgtcggttg ctccggcgag ttgtcagctg atagtccgga gcgaccggac
    34681 gcgcacacgt atcttcgtca gagtatcgaa ctgtgtcaat acgtgcaacg gaaaggagca
    34741 acagccgccg ttctcgtatt gcctgtggcc cttgtccccc atcgcgagga attgccggcc
    34801 gaggtggtcc tggaatattt ccggcgtgtg gaggcggaaa cggatatccc gctcatcatt
    34861 taccagccgc cggaaacgac ccgccgtttc cgggcaactc cgtcgctgat tgaggaagtc
    34921 tcgcgcagcg gacaggttgt ggggatgaag atttcaacga gcaggatgga cgtttttctg
    34981 agttgcagta aggcagcgca gaacgcgaat ttcgcgctca tcgcgggtga tgaaacggtg
    35041 ttcctgttca ctatcctcgt tggtggaacg ggcgtgatcg ggcaaggatg cacaacccat
    35101 ccagaaatac tgaaagcggt gtatgagaga gcgatggcgt ttgacttggc tggtgcttca
    35161 aaagcggccc tggacacctt tgccgcagta aacgcggcgc ctggcgagac cctcgacggt
    35221 gtggtcttcc ggtacctcca gaggcaaggg gtgaccgtac agccttattc aagggatggg
    35281 ggccgtccac cttcggagga aataagcgca cgctttgcct ccagtctgga cgcagtgagg
    35341 tccgggtacc gagattcaat ggtccgttag tgtagttttc cgattcaagg gagcctgttg
    35401 tgatgaagag atggactgat cccgttgtgc aaccgtacac cggtcgcgtg attcctgcct
    35461 acctccacgg ggtgattacc ccgatgttca cgccgtgcaa tccggacgga tcgttcgatg
    35521 aaaagggtat ccgtgcgttc gtggattacc tcatcggaac cggttgtatc accaccctgt
    35581 tcgcacgttc aggattggga aggatgtact cgttttcgta tgacgatgtg aagtggtttg
    35641 cggacgtcgt tctcgatcaa gcggcagggc gtattcccgt tatgatcggg acgacgggta
    35701 tctggggtcg agatttccat caccgtccgg ggaatgagat tttcactcgt cagagcatag
    35761 agctttcaca gtacgttcaa aaaaaaggcg cggtggctgc ggtgcttgtc cttcctatgg
    35821 cgctgcaacc tgaggaagga cgtcctctgg cggacacgat ttttgcgtat tacgaagcag
    35881 tcgcacgaga ggtagacctc ccgatcatcc tctaccagca accgggtttg ctggaggagt
    35941 actgtatgac tcctccactg ttggaacgcc ttctgaccat caagaacatc gcgggcatga
    36001 agtactcgac tggcgacatg gtgaagttca gcggtttgac gatggttgcc cggaataccg
    36061 atttcgcctt tatcgccggg gatgaacttg cgttcttctt cgccatgatg cttggagcga
    36121 gcggggttat cggtcagggt tgcgacgtga acccggagac actgcgcgca gtatacgaca
    36181 gaatgatggc ccgtgatttc gcgggcgcac ttgaagctca ctatgactgc atacgggcgg
    36241 ggcagatctc agaggggtac gattgcgtca tcagcgggtt ccggtacgtg gcggccaaag
    36301 gcgtcagggt gcaaccgttt actcgcggac acggtgtttc cgattatcag gtgcagcaac
    36361 cggtggttcc ggatgactgg atggcgagtt tctcgcgcga aatggacgcc cttcgtcgga
    36421 aatacccccc ggttaacccc tgggcgtagg tttggtcgtg acaaggaagg cgctgatcca
    36481 agcgccttcc tttgttcctc gccaccctat gccttcagtt cgccgatgag cgatcgcact
    36541 gaagcaatgt cctcctggat gcagaaatcc ctgagctcag agatcactct cggcgcgacc
    36601 cacgggtcga cgaagttccc ggtgccgatc tgaacggcgg aagccccggc gagcatgaat
    36661 tccacgacgt cagtacccgt catgatcccc ccgatgccaa ttaccggtat gcgtacagac
    36721 ctgctggttt tccacaccat tgccagagcg accggcttca ctgcgggacc ggacaagcct
    36781 cccgtcacgt tgccaagtac cggtttcctc gttctcgcgt caatcgccat tccccggatc
    36841 gtgttgatga gggaaaccgc gtctccacct ccctgctcca cgccgcgcgc gatttccgcg
    36901 atatccatga cgttgggact gagctttgca atgaccggca aggcgcactc ttgtttcacg
    36961 cgtcgaacga gttgctccac cagggacggc gcagtgccaa aatcgagccc gtgggacacg
    37021 ttggggcagg acacgttgag ttcgactgca gacacacctt tttcccgggc cacacgtgcc
    37081 gcgagtctga catacccttc cgcggtttcg cccgcgacgt tgacgatcac cgggcaagca
    37141 gcctggcgaa gctcgggaag cgcatcccgc agaaacgcat ccacgccggg attcggcaat
    37201 cctatggaat tcagcatccc gccaaccgtc tcacacactc gcggaggcgg attgcctgcc
    37261 cgcggcagct cagtgattgt cttggagaca attccaccca gaagcgagag gtcgtacaat
    37321 cgcgccatct cgcgaccgta ggcaaatgtc cccgacgcga cgagaacagg attggcgagg
    37381 tcaagagcac ctactgacgt acgaaggtcc gggctcaacg tcgtgcctgt catcggtatc
    37441 gatctcacgg agtcaacctg ctgcttcttt cagattatac ggatcaagtt cggtctctcc
    37501 cagcctctcg ggtgcttcca aaggcgttgg agcgtcggtg gctaaccttt agcttcatgt
    37561 tcgggcggcg agtaaccgat ttgcggggag taatgtggac tcgtcaaccg aaaacgatga
    37621 gtccggaagg aaggcaaatg gacgcgaaaa agaaagcacg ggtattcgtt cagacggcgg
    37681 cggcacctct gagcgaactc gaccgctggg tgcaagtcgg cgaagaagaa gcccgtgcgg
    37741 tgtacgaaat gacgttgcaa aacgagctgt ccgggggaac ggaacaggtt cgcgcgtttg
    37801 agaatgagtt cagggaattc accggcctca aacacgcgat atccgtgatc aacggcaccg
    37861 ctgcaatctg gagcgcgatg tacggttgtt atgtgggtcc gggagacgag gtgatctgcc
    37921 cggtttatac gtggctctgc agtatcactc ctgcgccgat gctgggtgcg aaacccgtgt
    37981 tctgcgacgt gtcaccccac acactcctca tggaccccga agatgtgaaa aagaaaatca
    38041 catcccgcac gagagccatt gttgcggtgc atttgtgggg aatggtctgc gacatgcagg
    38101 ctctgggtgc gataagtcag gaaaccggaa tcccgattat cgaggattgc tcacacgcgc
    38161 acggtgcttt gtacggggga cgggtggtgg gggcgattgg gaaagtcggt acatggagca
    38221 tgcagggttc gaaggcggtg agcgccggcg aaggaggagt gctcgcgacg gacgacgacg
    38281 aggtgtttga ccgagcatgc atgatcggac aggtgaatcg cgcggcaggt ctggatctcg
    38341 tttcggccaa atatcagaaa tatcagccgc tggggctggg tatcaaattc cgtgcgcacc
    38401 cgctcgggat tgggattgcg agggtccagt tgcgcaaact cgggcttctg aacgcaggca
    38461 gaggcgggta catcgaagca gttgaaaggg cactgaagga gattccggga ctcgatccgg
    38521 tcacgttaac cgacggcacg aaacgaggcg gttactacgg tttcccgacg attttccgcg
    38581 cagaacaagt tccgggggta acgcgggacc agtatgtgaa tgcgctacgc gcgcagggtg
    38641 tacccgcctc ttcaagtcct tacgacctcc tgcatcgcct cccgtatttt gccgaaggca
    38701 tggacgtgtt cggattcggg cgcggcgtta tgtgtgaggg gtacaacggg tataaggaag
    38761 gggatttccc cggagcggag gaaatgcaca ggaacgtcgt ctttctgccg gttctgactg
    38821 accccgtaga aggcgcaacg gaggaaatca tacgccgcat ccgcaagggg gttgagtccc
    38881 ttggattcta aggaccatgc cgcaacgcct ccaaggagcg aagacagctg gatcgtgttc
    38941 tcgtcacgaa ggacggagta cgcgtgtgcc gagataggtt ttgtacgacc cgacggcacc
    39001 ggcctccatt tcccgcacct cgacattgaa ggccaggtga gctggcacgc cggtcctgcg
    39061 ttcgctggtg gtgaccgcat aatcctgcac agcgtcgaga actccgagac gtggaaacac
    39121 cggtcacgat ccaatctgtg gatataccag atttccagtg gcgaactgac ggctttgccg
    39181 ctgaaacacc ggcccgccga tttcatgccg ccagcgcttc tgctgccggg cgatgaacgc
    39241 atagtggtca atccggtcat ccagggcaat cagtgcgtag tcaccatgaa cctggacggc
    39301 agtgacccgg ttcatgtcac gaaaccggag gacggcttcg cgtatggaat cgcgctctcc
    39361 ccggactgca gcatgctggc gttccatatt gcggggccgc ccaactactt tgcgtgcgtc
    39421 gccgacatcg acggagcaaa ccggcgagtc gtcgcacggg accctgacgt gcttttcttc
    39481 gctccgatgt ggtccagaaa cggcgactgg ctcgtatgtg aagggtgttt cagtgggaaa
    39541 gaccccgagc attttgcggc ggacctttac ctcgtacgac cggacggcag ggaattgcgc
    39601 ccggtaacgt ctggccagag ccactggttt gcggcggcat atggcgacga gaaaagtcgg
    39661 agtggcggat ccaacgtctc gcgttggtcg ccaaatgagg acgtggtttc gtacacgcgt
    39721 ttggccccgg gttcccggac ggcgtggaag cacattgaac agccgcaaga agacgaccat
    39781 ttcaatcgag cgtacgcgcc agaggaggct cggggcggaa cccagctgtg cgccctcaat
    39841 ccgttcgagc gggcgtgccg gcccctcacg gaagtgaggg aaggcgtgtg ggacatgcgg
    39901 gcggcgtggt cgtccgacgg tgcgttgatc gcgttttgtc gcgcacatgt cgggcaaccg
    39961 ccagggctct gggttatgag tgcggacgga ggacaggccc gtctgctgac ggtcgggaaa
    40021 gagggtctcg gcgcagacca cccggcgtgg atccacgcga gataggtatc ccgcttgtgg
    40081 aggccaacgc gcctaaagct gcttgtcgag gaaagacgga tatggaaccg caggaatctg
    40141 gacgcggccc ggttgattgg tacacatcgg acccgtggaa ggcatatctc gggtggttaa
    40201 gtccgcgctg ggccgagccc cgggaagact tcgatccgga ggaatggtgc gacgctttgg
    40261 tggaaggagg tttccagacg gctgttgttc atgcaaagca tcacgacggt atctgctttt
    40321 tcccctctcg gttccgatct gcgcagccgc cccgggattt cttcggtgaa atcgttggag
    40381 cggcgaaaaa gcgcggaatt cgcatcgtgg cgtattactc cacaacgttt gacacctgga
    40441 cgtgggagga acacccggag tggtcgtgcc gtgagcaaga tgggtccgta acggttctta
    40501 cgcgttttcc gtttcctatc ggagtctgtt gccatgtcaa tcccgggtac aggcaattcc
    40561 tgttgggcca gttggaggaa attcagggtg cgtatgacac ggacggcttc tggatggatg
    40621 gattcgacta taccggatac ccgtcgcggg cgtgtttctg ccatttctgt cgcgagcggt
    40681 tcgcgaaaga caatgcaggg atgtcactgg aagccgcgta cctgtcagat aagctgcgac
    40741 tgtggcaaag ggacgtgttt ctggaattaa tgaaggacat agtgacgatc gcgaatcggg
    40801 gacgggcgga tcgtgtagtc gtgtacaata acgccggaac aaaccttgaa ctcgggtacg
    40861 aacggatcga cgagctttgt tcgctgaatt ccatggaagc gcattctgct cttgggaaaa
    40921 gccttacagc gcggttgctc gcggggcagc gaaaggcgtt cgagatatac acaccggtcg
    40981 cagacaaggt gttctcgtgg acgccccgaa ccaccccgtt actgaaactc gaaacggcga
    41041 ttgtggctgc tcacgggggt tcgacgcttg cggggctgga tgttaccgcg tccggccgaa
    41101 tccccgggtt ccagaccagg cagatggcgg aagtgggagc attcgtccgc cgagtggctg
    41161 gcgtatgtca cgaggcagaa ccggtgtacg acgtcggggt gcttattccg aaggaccagt
    41221 ggaaggacga acggggtacg tgggccgtgt gtctgttgaa acatcaccat gcgttctgcc
    41281 aggttccact tcatgtgcgc gattattcca t
//
LOCUS       DAOSOO010000005        38944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1083_length_38944_cov_5.469310, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000005 DAOSOO010000000
VERSION     DAOSOO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 38944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38944
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_38944_cov_5.469310"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLT86_00720"
     CDS             <1..408
                     /locus_tag="PLT86_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35172.1"
                     /translation="FERVSIDKNIEDRFGKLGGTDWRERKAKHIDDDIAANPEGILVA
                     EYQGQVVGYVTITLDARTRLGRIPNLAVKAGLRGLGIGSKLIEAACEYMKSRGMTHGK
                     IETLDQNEVGLHLYPKMGFEEVARQVHFVKKLQ"
     gene            420..1478
                     /locus_tag="PLT86_00725"
     CDS             420..1478
                     /locus_tag="PLT86_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35173.1"
                     /translation="MLYKPDWDEAKTHLIAWWNRSHVDRATILVRAPRPTSLQPPPPP
                     KDVRQFWLDPDYRIAAAEYQMASTYCGGEAFPWFDTNIGPGSLALFLGSDAILHPTTV
                     WYTPIADRPGDVPPIRLDTSCEWWQKSVRLVREGMIRGEGRFLSSVPDLIENLDIAAS
                     LIGGQQLLYALVDDPGGVKRMVREINAAYFQCFDLMYEMLDGDRLGCCFCAFQIWGPG
                     KTAKLQCDFSDMISPEMFEEFVLPGLIEQTEYLDYTIYHLDGPRAIRHLDLLLSIPRL
                     NAIQWTPGDGNPEVGDETWWPMYRKILAAGKGVHLGTAYRDVEPLVKHLGNDGVYVAT
                     HAPSVEAADDLLRRAKTW"
     gene            complement(1521..1596)
                     /locus_tag="PLT86_00730"
     tRNA            complement(1521..1596)
                     /locus_tag="PLT86_00730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1561..1563),aa:Gly,seq:gcc)
     gene            1659..2408
                     /locus_tag="PLT86_00735"
     CDS             1659..2408
                     /locus_tag="PLT86_00735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT35174.1"
                     /translation="MSSYLVKPADICGDTAVLSGDEAAHASKSARKRPGDELVLIDGV
                     GTGYRCVVRTVEGDRVVCQIREVIPHMGEPRTHVTLAVGMVKGDRFDSLVETAVELGA
                     GAIVPLETRFSIVHHTSENRQRRWNRIAEAAAKQCLRSRIPSVEKPQPLRSFAERTGE
                     FDVVFVAWEGQQESANPNPVAPAVGSILLVVGPEGGFHDEEISLLRSKGAHTVPLGPR
                     RLRTGTAAIALLTLALQGVGEYARCLAPHVD"
     gene            2500..2790
                     /locus_tag="PLT86_00740"
     CDS             2500..2790
                     /locus_tag="PLT86_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35175.1"
                     /translation="MGEVRVTPERIHGFGCDGNGSSWIIGPDGVILKEASVFGEEAVV
                     ADVAIADATRRLALLSRELAPLGKWWNLGVKILGDLSSGEWTSPEIPGTPKK"
     gene            2798..3616
                     /locus_tag="PLT86_00745"
     CDS             2798..3616
                     /locus_tag="PLT86_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT35176.1"
                     /translation="MLKVRFAAAPILWRGVPFEGIAREVADIGFDGIEAPVTRYREDR
                     ETLRRLLGETGLALASTYTSGYYMDPGSREDEIRAVVEVAKLLPEFGCHTMISAPQGL
                     RKERKAYSLWEYRTLFEALNEIGRRIADFGVTQVYHNHAWTVVETRLEVDLLCAHTDP
                     RYVKMGFDTGHLHLGWSDPVELIRLYGDRVAYLHFKDARGRADQWPLMGKDDDVWCEL
                     GAGEVDLAGIVRELNARNWEGWLSYEQDKTAKTPRESASESLRYAKKLVAEFGS"
     gene            complement(3706..5496)
                     /locus_tag="PLT86_00750"
     CDS             complement(3706..5496)
                     /locus_tag="PLT86_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT35177.1"
                     /translation="MKPRISHSIPHLSAALALVFFGCSPVEKTPPAQNPAELPSPAGV
                     ETPADSEYGGRATKALAEGEGIRGTARASGTADIHATESRVRGLSFDGAQAAQVKAGT
                     HDDNEEYPSWVMFSSRKSLLPMVLPAAYHGRTVLRVIDSAGAPVMAQSYTVRDAGNAP
                     LWRGVTYANGETVLFPSVFWSDRATPASVVCHLSNGQEIQVPWRPSLDGFVTVQLDAP
                     RRTPRKIPVDVAFVLDATGSMGDEIQQLRDVLFSIHSRLQNAARAADIRFGLVVYRDR
                     GDAEPLRIVPFTANVDSFQLALESVSASGGGDTPEDVHAGLRATLKDLAWREGSIRNA
                     FLIADAPPHTDYGQEYDYLWASRTANSMGIRINTVGASGLDQSGEHAFRQISVATYGQ
                     FIFLTYGETGESSGMGSAADPGRVSHHTGANWSARRLDDIVVDLVRRDIAYQTEVPTL
                     VTDNPPPVDQHDHLAVRMENLWQQVQRQLAAFSGDTLTAVLLPFESTLDDSSHLEAYL
                     RDVSTETLLRLGSVRMVERDRLAQIIAEQSLSVSAAIEPSRAIELGHLLNSRVVFLGK
                     IYRLGTDRVVHVRAVETETGQIVAAARVRV"
     gene            5687..6727
                     /locus_tag="PLT86_00755"
     CDS             5687..6727
                     /locus_tag="PLT86_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35178.1"
                     /translation="MPLVFGIVGCRHGHVFSLIKELLSAPNTECAGVFDSDQDSLRAV
                     TSRFPVRAAASETELLDNPDVTLIGTAEINRKKAPLILNALNAGKHVIADKPLCTSLH
                     ELNAIERTATEKRLKIGLMLTERFGPANRRMKQLVSDGFVGHVANVMCWRPHRLGRAT
                     RPDWMFVDDLYGGILVDLAVHDADIIRWLTGGNFLEVTAYQYNMGNPADTDFSDIGVM
                     LGRLSTGATGFVRTDWFTPATSPVHGDTRFLVTGTTGMIEVRTSGDLWGGEAGKEPQV
                     LFISDTQPATRFDGIGPERPLVHDFLRSITDGRPAEITTADVFEATRTTLMARLSCKL
                     GRTVTRSESYLA"
     gene            6741..7532
                     /locus_tag="PLT86_00760"
     CDS             6741..7532
                     /locus_tag="PLT86_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35179.1"
                     /translation="MKLACQEGLVPGKSIAEKLANLQKAGYEAMEVWGGGLPDRVKEI
                     RAACKSSPVKISSVCAGYGGCPLDSDPAGRQKASDDIKRLLDATGEIGAVGLIFVPIF
                     GGPRIGDLSPWKTAVELEKDLLVELLSGWADHAAAAKTLVILEPLNGYETHLIKKLAD
                     GVDICERVSKPNGLKMMADFFHMGLEEPDVAASIKAAGKWLCHVHLSDHPRILPGYGH
                     SDFKKGFAALKSIGYTGFMALECGCPDADKMKAITKTAKFLKAQM"
     gene            7566..8537
                     /locus_tag="PLT86_00765"
     CDS             7566..8537
                     /locus_tag="PLT86_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35180.1"
                     /translation="MAVRIGFIGCGGIANHHMRTMAGNPDAEMVAFSDPDVERARAAA
                     KQYGGKAYSSYRTMISKGGIDAVFMCVPPFAHTDHEKLVAEAGLAFFTEKPIGLSLKR
                     VAENAELFAKKGIITAVGYNWRYSDGAAMMKKTLDGKKLAVVSGYWVGGTPGVMWWRQ
                     MKKSGGQVVEQTTHIFDMCRYVAGEIDVVFASGISGLTSQDELPLYDVHDASVVQLRY
                     RSGAVGNITSSCIVSQGVGTQVRAYARNLVVVVGEGRCQVSIPGEEHIYKDARNPTAH
                     EQEAFLCAVAKKNGPPILSDYADAMKTLRVTLAANKSMETGKPVKLA"
     gene            8716..10065
                     /locus_tag="PLT86_00770"
     CDS             8716..10065
                     /locus_tag="PLT86_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35181.1"
                     /translation="MKFLRSQSFPVLTILLAAVASLLFEACSSTKSAVGGGLLTDVTG
                     EFSRRDTIDIPLSGVSPATVACFPVHISFSSSEALLAGEANGLRATVLLKFSPIVSWT
                     EERYYTLNDQAALDTLTFTPVSVVTDSSSQLRLTYYGHVADSSFCAVQTRLVVSSWKE
                     EDSVMTALPAMNAPPFSPVRVDTTASGSVVYLPFAPASLTQLIADSVSLAVETTSGSS
                     MVRFLSQEGATTYSPQLTLWIKANVTSKRPEIAAHDTLIGLTLRPIHDCYRLQRLVPA
                     PAPSAGSLRISAGDAWRGYVRFDRFPIPGVSDSAGVVRARATANSAQLQLILGPDGGG
                     SFKRDSAAILIYPLTDPFDPDSARAGLLNFRTGRLYGVGFNTAETRAAPDVRVFEIAD
                     LINQWWVNPSSNNGLLISLSGEAQLVDALEIRSMRLIVTTTMPPQLIGKPASGEAPR"
     gene            10062..11315
                     /locus_tag="PLT86_00775"
     CDS             10062..11315
                     /locus_tag="PLT86_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35182.1"
                     /translation="MRYRLLVPHLLVFAAACHASSIFSYHPRGLGYAEWKGDARTMGM
                     GGVSLANSGEDGATSANPAGIAGCERTALSASFLSQKRSVKDYLGYAGTFYDQYPRFF
                     RAVVPIGRGIVSSFGLEPVGDVRMIWANRNTENGVSYLDSLEASGGAWSFGAQVARSF
                     GPISLGLKAQVVRGNMTTEWRRTVVDSAAPLATSALAMRRISGAAFSLGSAYRVSEEW
                     AIGGRLDFPSNLSETDITSYGTRIPNQYYKSHTDVVMAVASDLDDTTSSTAKLPVGFG
                     AGVAWRPGERLLVSLDGEFRRWSDVDPAFRNTWCVAGGVEFRVSDDYRSFLLWQLPYR
                     AGVRWEQHYVPALSSHGGATYPDGKYLTLGAGIPLGRNRGRLDYSVEYGTRGTISTNL
                     VREKLWRHTISVVGWERWFERSTRR"
     gene            11436..12209
                     /gene="bamD"
                     /locus_tag="PLT86_00780"
     CDS             11436..12209
                     /gene="bamD"
                     /locus_tag="PLT86_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOT35183.1"
                     /translation="MLLRRTPIRLLVCITVLSVISACGPKRTHLLTCEEYYQRGINAL
                     EKKKWLKAQENFEKITMNFPGCDLVDDAQYMLGETYFRQDKFIEAQFEFRRVVEDFRQ
                     SDRTEDAQFKMALCSHRQALPAALDQTATQDAIYRFQQYLEDNPSGRWAGEARRYILE
                     NRKKLAQKDFNTAQFYARQGYNEGALIYLDHVIAEYPDTGEWLERARFLKATILAQRG
                     QTQEALTLLRAINLDALPGKVQKKVRETTARIESPQENW"
     gene            12220..12873
                     /gene="nadD"
                     /locus_tag="PLT86_00785"
     CDS             12220..12873
                     /gene="nadD"
                     /locus_tag="PLT86_00785"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT35184.1"
                     /translation="MSESNDVDTPSRNRRVGLLGGTFDPPHNGHLALALYAQEHVGLD
                     QIWFIPSAVPPHKMQKTSTPAALRWEMTCAAVNPYSPRLVVSDVELKRPGPSYTYQTV
                     QRIRRMLGNEAELFWIIGRDNIAEIPLWMEPERIVEEATVIAGGRPGVPLPDDIPGWL
                     ARRLIVLQGPDLPVSSTAIREHASRGIINPEWVPGPVSEIIRREGLYGYPRTAPLPD"
     gene            complement(12892..14865)
                     /locus_tag="PLT86_00790"
     CDS             complement(12892..14865)
                     /locus_tag="PLT86_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35185.1"
                     /translation="MSDHFARIQSLGNHGHDDVSLRSIVLGTLACIVVVVWAPLSEFL
                     VGASRLNLSQLPVVAMGLFFGVVLLNSVVGRIAPVLRLRPAELITVFSMAFVGSIVAT
                     SNLLNWPLGIMSVPYYVATPENRWMSDLWPYLRQWAVVQGPRDELRWAFVGMPDGAKI
                     PWGIWIVPMFWWGTFLGAVALVAICLASLLRKQWADNERLAFPLVQVPLELITSPSGK
                     WGISETLRKRSFWVGAAIPLFVILFNCLNYFLPQFPRLPVMDGISIQLGPNFPAFGIK
                     LNMYVLGFAYLVNTNVLFSVWFWDIILVLESGFFRSVGYTLGDADDPYSSRDALTSWQ
                     GFGGFIVFVLVSLWMARNHLKMVWYAVIGKIKADDENELLSYRWAVIGLFVAVVYMTG
                     FMMSLGMSWEMALVFLFGAFVAYLGTTRVIAQTGLVYMQSPLTPTMFTFGAFGTVGVP
                     AQEIVGMVGTYSLVVNGRAPFMPAIFHISWLGAKIGARGRRMLTVLIVALIVAYACGS
                     FYYLIIAYNEGATTFRSTHFAFHGEQVYNMIIQKMQARVAVDPGRWTFLGAGALVMSI
                     LTYLQYRFPAWPLHPIGFPIAAANNVHRTVFAIFLAWLIKTIILKIGGVEAYERGRPV
                     FIGMAAGYALGILLSFFVDWMWFPGAGHQIHNW"
     gene            15160..16512
                     /gene="nuoF"
                     /locus_tag="PLT86_00795"
     CDS             15160..16512
                     /gene="nuoF"
                     /locus_tag="PLT86_00795"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT35186.1"
                     /translation="MAARQNRMILDQCEIPGIRSLAVARRYGAYTALAKALTTMTPDQ
                     VVEQITRSGLQGRGGAWFGTGLKWSFMPKEPRVESFLCVNADESEPGTCKDRYLIEKN
                     PHLLLEGVLIACYAIRASTAFIYVRGEMWEGMEVLETAIRELVTANLCGKNILGTGFT
                     INVHVHPGAGAYICGEETALMESLEGKRGYPRNKPPFPAAYGAFGHPTTINNIGTLGY
                     VPWIINHGADAFKGIGDPNFPGTLIYQVSGHVNRPGLYEAELTVTLRDLIDMAGGVRS
                     GHRLKAVIPGGTSMPLLTADDLDTVMSPASFAPRGSLKSGLGSAAVIVLDDTCDMVDV
                     ALNMMRFYRHESCGQCTPCREGTKWVCDMIERIHRRRARPGDLEVLEQVLVNVGDIDR
                     FEFNKTICLFGVSFGWPLTMLIRRFRSEFEAAVAESTPAPVPLPVPASVLPAGDLRAP
                     "
     gene            16601..18082
                     /locus_tag="PLT86_00800"
     CDS             16601..18082
                     /locus_tag="PLT86_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT35187.1"
                     /translation="MPTMVIDGREVTAPPGANLIEAAKLAGIEIPHFCYHPALKVVAS
                     CRMCFVEVTQEVRGKATTRLTTACNTPAADGMVVRTDTQPVKKAQNDILEFLLINHPV
                     DCPVCDEAGECKLQDYAFAYGTASSRFAEHKRFRHAKKLGSGIRLYTNRCILCDRCVR
                     FLRDFVGTGELSVTDMGNGNEISVFPGRPIDNVLAGNLPEICPVGALISEDFLFKARV
                     WNLSAVDSVCPGCSRGCATSLDVKDNEVQRTRARENQAVNGYFICDDGRYLYHVIRHE
                     TRLEHPAVREGDQLRESNWDHALDVVVSILAEFPERVVILLSPFLTQEEVRAGADLSE
                     VLRGSVLGHVPNAVQEAPVLFPGGFAIDGDKAPNSTLVKNIAPVPLQELAITKDTVVL
                     FFNSSLRTGSVSPLDAEKLRRAGFVAVIDVLRNDITAVADVVLPGRMWAEKSGTFSNS
                     AGMAQSFGAAVLPPPFTRDESDVIRDLTRRITTRISTGCGAKS"
     gene            18079..19137
                     /gene="nuoH"
                     /locus_tag="PLT86_00805"
     CDS             18079..19137
                     /gene="nuoH"
                     /locus_tag="PLT86_00805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOT35188.1"
                     /translation="MISQEQLIVSLALGLVLMLAMTAGAGVFTFYERKIAGWTQGRYG
                     PNRAGPKGYLQFLADGIKFALKEDVIQDYVVKPVYVIAPGLMMIPALVLYAALPIGKP
                     FVWNGNEVHLQVVQANVGVLYLLAVASIAVYGIVFGAWASNSRYPFFGGVRASAQMIS
                     YELAMGLSIISVVLLTSEPGINALSISNIVAGQTGYWFGFIPRWNLFANPVAFVVFLV
                     CMFAETNRLPFDLAEAEQELVGGYHTEYSAMKFASFMLSEYVNMITMSMVMVTLFVGG
                     WHFPGIEAIGSQVIQVLLSIGAFGIKVLFFCFLFLWVRWTLPRFRYDQLMHLGWKALL
                     PVALASALGTAIAIALRV"
     gene            19190..19807
                     /locus_tag="PLT86_00810"
     CDS             19190..19807
                     /locus_tag="PLT86_00810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOT35189.1"
                     /translation="MATINVQRPARLTLAERLYLPAILNGLRLTWRHIWGERVTLQYP
                     EEEREFKRVYRGVHRLNKDDKGRVKCVACEMCAASCPAHCITIKATEVEWPERDKIPK
                     IFEIDELKCIFCGYCVEACPEDAIHMTRHYHLIARSREDMIFGLDKLLAIYDETKEQE
                     VLRGVDFGVIRARIPGKEAGNPDEVQKLAYPVVGVVTKAETHHAQ"
     gene            19830..20420
                     /locus_tag="PLT86_00815"
     CDS             19830..20420
                     /locus_tag="PLT86_00815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT35190.1"
                     /translation="MSLVIFVVFGCVAVGCALAMVFARNPVYGAIALIVCLAALAGEY
                     LLLDAPLLAAFQILVYAGAIMVLYLFVIMLLNVRREEGCRWCTDWRTYLGFVLAGLIG
                     LSFLFKASNVAHPAPPVGFHVYDVTGVAYTMFTDPTLLFIIEALGVLLLMSVVGAIYL
                     GRRFSAEEELLMKHDRPAGSNSTGTAGDAPCGETAP"
     gene            20417..20719
                     /gene="nuoK"
                     /locus_tag="PLT86_00820"
     CDS             20417..20719
                     /gene="nuoK"
                     /locus_tag="PLT86_00820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT35191.1"
                     /translation="MIQTIHVLWLSTILFVIGTAGVLIRRNAIVMLMSVELMLNAVNL
                     ALIGFARQFRDVTGQVYVIFIMTVAAAEVAIGLALFVAVFRHKRTLDTQKIDLLRW"
     gene            20795..22684
                     /gene="nuoL"
                     /locus_tag="PLT86_00825"
     CDS             20795..22684
                     /gene="nuoL"
                     /locus_tag="PLT86_00825"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOT35192.1"
                     /translation="MTTAIALIPFWPLLGFLINILFGKRLRSASAYLAIGMLALSFLT
                     SVAVFTRFVGDPASVLVPLFSWMSVGALNVEVNFLVDQLTVVMLLVVTGVGTLIHVYS
                     VGYMHGDERYSRFFAYMNLFAAMMLVLVLADNYVVMFVGWEGVGLCSYLLIGFWFERP
                     FGSGTTNDAARKAFIVNRVGDFGFLLGVFLIWTAVGGVSFQQTSSAVESGYVTPGVAT
                     IATLLLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARSHFLFD
                     IAPFSQNVVLWVGALTALFAASMGVQENDIKKILAYSTISQLGYMFMGVGAGAYAAGV
                     FHLMTHAFFKALLFLGAGAVIHALHGEQDITRMGGLRNRMPLVHGTFLIGALALAGFP
                     LTAGFFSKDSVLAGVYAHGDFAVWCIGVLTAGVTAFYTFRLYFLVFSGSSRDEKGRDL
                     HAPASSMTSILLILAILSLVGGYVELPFHILAAFSAFLSPVVGMSSHEGIPELALMTV
                     VFLFVLGGIGVSWAWYGRREQDATALAAEPANPFLRTLRRKWYVDEIYEVLLIRPIRW
                     TSRTVLWRVIDEWIIHGFLVQFCGGYLWKAIGFVVSFSHTGRVGSYAFGIVVGVLVII
                     SLIAR"
     gene            22715..24313
                     /locus_tag="PLT86_00830"
     CDS             22715..24313
                     /locus_tag="PLT86_00830"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT35193.1"
                     /translation="MALSTLILLPVIAALILGFLPESRKELVRNVALVFALMVFAYSV
                     GMYFQFDPSTPRLQFVENRSWIDAIGARYHLGVDGVSIFLVLLTTLLTPIAILASYAA
                     IGERVRMFNAMLLLLEAGTLGVFLAQDLVLFYVFWEVMLVPMYFIIGIWGSSERLKAT
                     FVFVLYTMAGSVLMLVAIVALAIVNAQNVAAALSAPGAYMGYVSFDISSIRTELLTGT
                     QQIWLFAAFFLAFAIKVPLFPLHTWLPLAHTEAPTAGSVILAGVLLKTGGYAIIRFCL
                     GLFPEAAVVYAPYINTLAVVGIVYGALVSLVQEDLKRLVAYSSVSHMGFILLGVFSFT
                     QEGMTGGIVQMVNHGISTGGLFLCVGMLYERSHTRKLEELGGTATAMPLFAGLFLVIT
                     LSSAALPGLNGFVGEFLALAGAFSNKATRWFSVVGATGMILAAAYLLWMFQQAMYTTK
                     PGVWVKRTWGDLTVREVLVLLPIVLSAVGIGVYPAPLLKPIAASSQLVLQRMNLRPNG
                     PIHAIPSGVDDLATTSPDLPEASR"
     gene            24310..25755
                     /locus_tag="PLT86_00835"
     CDS             24310..25755
                     /locus_tag="PLT86_00835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOT35194.1"
                     /translation="MISASDIAVLLPEIVLVAGAIILLMLDAFSPRITRRFGALLASV
                     VLFAALLAVQQGEPSKFPAFGALVSDAFTDMVAVSVLLFGFVAVISAWSYLEERGLAS
                     GEFMAAMLATSAGMILISRSSHLMVIFVALEILSIGLYMLIGYDRTDVLSAEAGLKYF
                     ILGAFASALFLFGAVLLYGVTGTMQLTLEQLPFHTRAGQVGIALCIAGLGFKISAAPF
                     HLWTPDVYQGAPTPVTAFLSSASKIAAFAALLRVVLPSFSPFAEGWHAVWVALAALTM
                     FVGNVAALVQENVKRILAYSSIAHVGFMLMALAGGATVGPVGIEGLLFYLLTYAVSAA
                     GSFAAVAVIPGSPDLHTIRGLAKSHPSIAALFALFLLSLAGVPPLVGFLGKLYAFRSA
                     VDAGMYKLAAFAALNTAMGMYYYLRIIVRMYMEQRAEPAAFVVPQVAYPVLVLAALAV
                     VLLGLFPDPVLVLVRQSAEAVVPLARAPVAF"
     gene            25887..27098
                     /gene="tyrS"
                     /locus_tag="PLT86_00840"
     CDS             25887..27098
                     /gene="tyrS"
                     /locus_tag="PLT86_00840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT35195.1"
                     /translation="MLPFNEQMDLIRRGVAEIVSEDELAQRISEAVRSGKPLRIKYGI
                     DPSGKDVHIGHMVPVSKMRDFQDAGHHGVLIIGDYTAQIGDPTGRNESRPSLSKEQVL
                     ANAATYVDQLMKVLDPEKTEVRYQSEWFENFSLIQMIQLGQQFTMAQMLSHETFAKRL
                     EEGLPLAMHELMYPMLMAYDSVAVQSDVELGGMEQRFNILQGRDLQRAMGQRPQIAVL
                     SPMLRGLDGGPKMGKSLNNYIGVNDSPADMFGKIMSIRDDLLIDYLTLAAGVSQDAVR
                     AAEARLRRGTNPMEIKRELGRAVVQRYHGAQAAIAAEEEFLGVFSRRDQLPEDMPEFT
                     LDENPVRIVDILVKTESAPSMSEARRLIQQGGVTLDGYRVTDANAEIFVKDGAILKVG
                     KRRYARLRLKE"
     assembly_gap    27229..27328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27329..27807)
                     /locus_tag="PLT86_00845"
     CDS             complement(<27329..27807)
                     /locus_tag="PLT86_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT35196.1"
                     /translation="MGQLEGKTAIVTGGASGIGRASAILFAREGANVVIGDIVDEKGE
                     QVAATIRSEGNRGLFVHCDVSDTRQVAGLVSRTVSEFGAIHVLHTNPFYSVGGVVETM
                     TDEVWDRTLAVTLNGVFYCSRAVIPEMIRAGGGSIVHTSSVAGVVSLAGTAAYCAAKG
                     "
     gene            complement(27844..29043)
                     /locus_tag="PLT86_00850"
     CDS             complement(27844..29043)
                     /locus_tag="PLT86_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35197.1"
                     /translation="MPESPRVAAIITTYFPNSHADVIVTRFLEGFPSDEGVIPPQARI
                     VSMWLDQIAPNDIGLAKAKEHGVPVFQSIRAALTLGGSDLAVDGVLLVGEHGDYPRDE
                     WDRQMYPRRALFEQICGVFAETKKSVPVFVDKHLSYNWPDAAWMLERARQLNVPLMAG
                     SSLPVGWREPELELDLDCDLTDALALGYGPLEAYGYHTVETLQCMVERRRGGETGVKA
                     VTCLRGDDVWKAGENGVWSQDLFEAALKTVFTKKDEPPRSGCAAPFAFVIEYFSGLRA
                     TVIMLDGYIVDWAFAGRVDGKVVATEFALEYGPPFSHFGYLSRNIERMFITGVPQYPV
                     ERTAITTGVISAVMESCAKGGARISTPYLAIPYRSYETPPIRAYGHKRPGKTGEGYKP
                     IGYSDEG"
     gene            29163..29477
                     /locus_tag="PLT86_00855"
     CDS             29163..29477
                     /locus_tag="PLT86_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35198.1"
                     /translation="MSFTETLYHIFPALGILGLIALWILAMLCGMSYFSAKMPDAPSP
                     TRWKPAVVRFGMVAFMALVGILVAQRNGVWPDYRLAVGLAGAVAGLLVDLLWRVFTSR
                     MT"
     gene            29684..30376
                     /locus_tag="PLT86_00860"
     CDS             29684..30376
                     /locus_tag="PLT86_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35199.1"
                     /translation="MQVSLFVAWDTTKGLATEAAGGPPSFASAYIPPGTNEYVIENIT
                     FGTYAALAASWRDTTMTGAAATTTIGAYGADPQAGDNTPLSITLSGSQPDLEIDIPVD
                     YSRAKVSTGPTGSISGTLTLSGTWPTRAVYLVVMPANKIGPPPAFAAMGQPVTMVALP
                     QFSGSKAYTIPNVPYGQGYAVAAYTFTPPMTLGFIGAYGVDLPADATLDSVTVGNGGA
                     IDLRNINFAIRP"
     gene            30423..32621
                     /locus_tag="PLT86_00865"
     CDS             30423..32621
                     /locus_tag="PLT86_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT35200.1"
                     /translation="MLRSGVIVLLLSVRFVGAAEIAGKVTNEQGAPLPYAPVQVRQEE
                     SSSPASGVNADSTGVFVIRNVPPGRYLISADYVGHDPWREIIELREPLRTVEIVLRTR
                     PIRGQDIVVTAVRRLEATTAASVSVSLVTDDELEYRGVRSLDQALETLDGLQVFRSHS
                     VATNSVSIRGSSDVLGGGVGNRVLLLVDGRPALNTDSGGQSWSLVPIGAVERVEVVKG
                     AFSALYGSSAMGGVINLITRDPLGTPETRLVARYGAFEKPPAWMQYRSGLADFAETSV
                     LHADHRGSTGWLVYAAHNRSDGHREASDYSVWQAYGKVAHTVFPGLEIGGSVGGGRSH
                     AGYPHRWKSLLEPLKVPDEKKQDRQNKGWWNLDLTTKAGTLEHGLWNAGLYTYGMESA
                     TQQPVAGLGSSVTSQRMGGRLQWQKQLAGFLTQTIGGEGSLDFVRSDSVLYGNRSARN
                     AALFSLSEVLFATGQRLDFGIRGDYTSLPGKATETLVNPKLGLTIPLGKTFFVRASVG
                     RAFRSPSIAERYLVLEPAGGTEFAPNDSLRPEKMKSYELGLTHDLSPVLKMDVAAFVN
                     DYDDWIYWRELPVQTDGRYRFQVANLLKVRMSGVDAQATLEPFQGFSLALNYLYLRAE
                     DRTPGRSNPTIPYRPRHTFSGVVVFTHGRLRNTVVVRGRSRVEEEVFAAYQVDAPRGF
                     VLTNLRTDYSLRRWLTFSLEANNVFNVQYEEMARFRMPGRSFSVGLAMKR"
     gene            complement(32869..33555)
                     /locus_tag="PLT86_00870"
     CDS             complement(32869..33555)
                     /locus_tag="PLT86_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT35201.1"
                     /translation="MAKGQLTPELIRKLESVDSPTISNAIERFKVRSRREGYMGPQIV
                     CRFPHMGRIVGYATTCTIVEYDENFPPDPRERFKWLESIHNSPKPAICVVKDTCHRKG
                     WSSHWGEIVGTQVQVLGARAIITDGAVRDLEALEAMGMKTWSEYVVVSHGWIDVGKAD
                     IPVEVGGLVVNPGDILHADMNGVVSIPAEVLDDMPAAIDDVLNKEQTFIKSMRENGYD
                     IEAHRRQLEH"
     gene            complement(33589..34386)
                     /locus_tag="PLT86_00875"
     CDS             complement(33589..34386)
                     /locus_tag="PLT86_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35202.1"
                     /translation="MVRVKDKLWIFAVEQACDDTYYNIPITRVTPVEGAYYLGVERLA
                     MIVSREGPYPPLEPYLRIMRPLKEVVWSIVDSGGVTGWAQGKELDMLCDLACRFPNIT
                     GIFMDDFFNGKPEDGYAVHSIEKLTRIREKLVLPHRTLDLWAVLYRHQLDLPVIEHLN
                     MCDVVSFWEWWGRELPHLETNLEKLERLVPDKRRSLGIYLWDYGDRAPMPLDLMQHQC
                     RLGLEWLKSGRIDSMMFLTNYLLDQDFEAVEWTREWIAEVGDEVLAS"
     gene            34593..35621
                     /locus_tag="PLT86_00880"
     CDS             34593..35621
                     /locus_tag="PLT86_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35203.1"
                     /translation="MPIELWDRAQWQEFSAARPSPFFAQTQWFEPTCRLMNAEPMYLV
                     LMSRSNPAFCVSLARKKRLGQTLLLTPAIAPYSAWIENFPDDLAPERREARRLEAMGE
                     FAAWCEQHAAYCRIVLPPQVVDVRPFLWRGWRTEVRYTYKIPLEHGVHPVFRQNVRRN
                     LRDAREANLRTECLTGEHAHGALRETVAATFRRQGEAPPLPAQAWDDYLALLSVCNNV
                     ATMGVFRGDAVLATIAIGFDPPTATGAGQSAYELIAGTTDEGIPVGASAMALCSAFEF
                     ASGKVGEFDFAGANMETIAHYKRGFGGTLTPYLAVEWSRSRRASFVAKTAPAWKRKFR
                     TLLPKGSR"
     gene            35618..36790
                     /locus_tag="PLT86_00885"
     CDS             35618..36790
                     /locus_tag="PLT86_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT35204.1"
                     /translation="MNARNGGGIAVDMVVYNDLRHDARVFKEATSLIAAGYNVRIIGM
                     ETPSTPPLTGWEEVTVKRIPVGTSDSLRIRYACFWRRAFSALMERRARIIHSHDLDTL
                     FPAWLAASLTRAQLVHDAHELWVELPSLVGRPVVRAAWQGFAKTLIPRSDAVITVCEG
                     IADELRARYGVGAVVLRNLPKEIGPVEPAPIREMTGCPPSRVLVLYQGGFLPGLGIER
                     AIDAMTLLPDAHLVLIGAGPLRKDMEHRAAASPARERISFLDAVPFKDLPPYTAACDI
                     GLFLGESAGMNLQLALPNKLFEYIAAGVPVVATGWPEIRRVVREYDVGVLVEPGCDLE
                     AVANAIRRAVSDRKRLSANCVKAARELTWERESDRLLEVYARLSARNSSPENRNDG"
     gene            36783..37724
                     /locus_tag="PLT86_00890"
     CDS             36783..37724
                     /locus_tag="PLT86_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT35205.1"
                     /translation="MAEITVLMPTYNRPEHLSAALETLAGQFEREVPFSVVVVDDGSV
                     PAVELDPGAFPFRLIFLRLEKNCGRSAARNAGLALVDSPLVVFLDDDMRVLPGFIRAY
                     QEAIDPAGRAVGLGSVCFHPDIPRDALTLYLETRGIAKLKADEPIPFRYFLTYNSAVP
                     TFLLREAGGFSERVRAWGGEDIELAWRLQKHGARFVRVPQARAYHAHRRRLEDVLSLS
                     ESFARECMPVLFDEDPVFTTQLRADLLGPVRYAKRNRWKRSAIRFVTTPCVSRRVEAL
                     VKRHLGARWPMWVFDYLIASAWRRGLDQAHARGEERE"
     gene            37721..38674
                     /locus_tag="PLT86_00895"
     CDS             37721..38674
                     /locus_tag="PLT86_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOT35206.1"
                     /translation="MTASRLRLLTVMALLFCGCGGYQLESGLSALAPTRAVRLVFMRS
                     FGERGSSSGCFRQPRGIACASFGHWYVADTGNDRIQEFDGEGRYVREIGGFGWSDGQF
                     NRPAGIAAGRNLDIWVADTYNRRLVRLGQDLVWAGVINKDAEGGPLGYPNGIALNVDA
                     SIWCTDIDADRLIQLYQETPSELGARIQSTGILSDPAGVACSPDGNIVVADTGNDRIA
                     VFDRFGTLLRTWGEGILLRPRGVNVTPQGDILIADTGNNRLVFLNRMYHLTGEHGLRG
                     NEDGEFLEPCSVASDPAGNVVWVADTGNNRVQVFRVIRTTE"
     gene            complement(38707..>38944)
                     /locus_tag="PLT86_00900"
     CDS             complement(38707..>38944)
                     /locus_tag="PLT86_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35207.1"
                     /translation="DSFDQAIFAHTSPVYVQTGALSPAREEAARFFADVIRGSVDWVN
                     TKGRFHNKQQRTTVLNLFREGAAVYEGMLRKAHT"
BASE COUNT         7889 a        11112 c        11589 g         8254 t
ORIGIN      
        1 ttcgaacggg tctcgataga caaaaacatc gaagaccgtt tcggcaagct gggcggcact
       61 gactggcgtg agcgcaaagc caaacacatc gacgatgaca tcgccgcaaa cccggagggg
      121 attcttgtcg cagaatacca ggggcaggta gtgggttatg tcacgattac gctcgacgca
      181 cgaacgcgtc tcggaaggat ccccaacctt gcggtgaaag cggggttgcg tgggctcggc
      241 atcggaagca agctcatcga agccgcctgc gagtacatga aatctcgcgg catgacgcac
      301 ggtaagatcg aaacgctgga ccaaaacgaa gtcgggcttc atctctaccc caaaatgggc
      361 ttcgaagagg tagcacgaca ggtacatttc gtgaagaagc ttcaatagcg gaggcaaagt
      421 tgctgtacaa acccgactgg gatgaggcga aaacgcacct gatcgcgtgg tggaacagaa
      481 gccatgtaga cagggccacg attcttgtgc gtgctcctcg gccgaccagt ttgcagccgc
      541 cacctccgcc gaaggatgtc cggcagttct ggctcgatcc tgattaccgc attgcggcgg
      601 cggaatatca gatggcgtct acgtactgcg gtggagaagc gttcccgtgg tttgatacga
      661 atatcggccc gggatctctc gcgttgttcc ttgggtcaga cgcgattctc catccgacta
      721 ccgtgtggta caccccgatt gcagacaggc cgggcgatgt accgccaatc aggcttgata
      781 cgtcgtgcga atggtggcaa aaaagcgtca gactggtcag agagggcatg attcgcgggg
      841 aaggcaggtt cctgtcatcc gttcccgacc ttatagagaa cctcgacatc gctgcttcgc
      901 tgattggtgg ccagcagttg ctgtacgcac tggtggatga cccgggtggg gtcaagcgaa
      961 tggtccgcga aatcaacgcg gcgtacttcc agtgttttga tctgatgtac gaaatgctgg
     1021 acggtgaccg ccttggttgt tgtttctgcg ccttccagat ctggggcccc ggcaagaccg
     1081 ccaagctcca gtgcgatttc tctgacatga tttcgccgga aatgttcgag gaattcgtgc
     1141 tcccgggatt gatcgaacaa acagagtacc tggactacac tatttaccac cttgacggtc
     1201 cccgcgccat ccgccacctt gacctcttgc tttcaattcc gcgactgaac gccatccagt
     1261 ggactcccgg cgacgggaac ccggaagtgg gagatgagac gtggtggccg atgtacagga
     1321 agatccttgc ggcgggaaag ggcgttcacc tggggacggc atacagggac gtggagccgc
     1381 tggtgaagca cctcggcaac gacggagtgt acgtggctac tcacgcgcct tccgtggaag
     1441 ccgctgacga ccttctgagg cgcgcgaaga cctggtaaaa aaagagagcg gctcgctggc
     1501 gcagcgaacc gctcaaaaaa tggagcgacg gacgggattc gaacccgcga ccccaacctt
     1561 ggcaaggttg tgctctacca gctgagctac catcgcgtgg attagagagt atagtgaacc
     1621 cggttgaacg agtcaaggtt tcaaactacg gtgcttccgt gtcatcctac ctggtaaaac
     1681 ccgccgacat ttgtggcgac acggccgtac tctccgggga tgaagcagca cacgcctcga
     1741 agtccgcgcg caagcggccc ggtgatgaac tcgtcctgat tgacggggtg ggaaccgggt
     1801 accgttgcgt cgtgcgcacg gtggaaggcg accgcgtcgt ctgccagata cgcgaggtga
     1861 tcccgcatat gggcgagccc cggacccacg tcactctcgc tgttggcatg gtgaaaggcg
     1921 accgcttcga tagcctcgtc gaaaccgcgg tggaactggg cgcaggtgcc atagtaccac
     1981 ttgagactcg gttctcaatt gtgcatcaca cctccgaaaa tcgtcagcgc cgctggaaca
     2041 ggattgcgga agccgccgca aagcagtgcc tccggtcgcg aattccttca gtggaaaaac
     2101 cccagcctct gcgctcgttt gcggagcgaa ccggcgaatt cgacgttgtt tttgtggcgt
     2161 gggagggtca acaggaatca gcgaatccca atccggtagc accggcagtc ggaagcatcc
     2221 tccttgtggt agggcctgag gggggatttc acgatgaaga aatcagcctg ctccgcagca
     2281 aaggggccca caccgtgccg ctcggcccac gtcggcttag aaccggcacc gcggcaattg
     2341 ccctgttgac cctggcgttg cagggagttg gcgaatacgc acggtgcctt gccccgcacg
     2401 ttgattgatc ctctttcgga gattctccgg tgaaagaacc cccgagcatc ctgcgtactg
     2461 ctgtgcagtc caaatcatgt gatctgagcg aacagccgca tgggggaggt ccgggtaacg
     2521 ccagagagaa ttcatggatt cggttgtgac gggaacggca gcagctggat cataggcccg
     2581 gacggagtga tcctgaaaga agcgagtgtc ttcggcgagg aagcagttgt cgcggacgtt
     2641 gccatcgcag acgccacacg gcgcctagct cttctgagca gggaattggc cccgctcggc
     2701 aaatggtgga atctcggagt gaaaatcctc ggcgacctct cctctggaga gtggacgtcg
     2761 ccagaaattc caggcacacc aaagaaatga gggacgcatg ttgaaggttc gttttgccgc
     2821 cgcgcccatc ctgtggaggg gcgtaccgtt cgaagggatt gcacgcgagg tggctgacat
     2881 cgggtttgac gggatcgaag ctcccgtcac gcgctacagg gaagacaggg aaacgcttcg
     2941 ccgcctcctg ggcgaaaccg ggctggcgct cgcaagcacg tatacatccg gatactacat
     3001 ggacccgggc tcaagggagg atgaaattcg cgcggtggtg gaggttgcga agttgctgcc
     3061 ggagttcgga tgccatacga tgatcagtgc accgcagggg ttgcggaagg agcgtaaggc
     3121 atactccctc tgggagtatc ggacgctttt cgaagcgctg aacgaaatcg gtagaagaat
     3181 cgctgacttc ggcgttacgc aggtgtacca caaccacgcg tggacggtcg ttgagacgcg
     3241 gcttgaagtt gaccttctgt gcgcacatac cgacccacga tacgtgaaaa tgggatttga
     3301 cacgggtcac cttcacctgg ggtggtcgga ccccgtggaa cttatccgtc tgtacggtga
     3361 tcgcgtggcc tacctgcatt tcaaagacgc tcgcgggaga gcggaccagt ggccgctcat
     3421 gggcaaagat gacgacgtgt ggtgcgaact cggtgccggt gaggtggacc tcgccgggat
     3481 cgttcgcgaa ctgaacgccc gcaactggga aggctggctc agctacgaac aggacaagac
     3541 cgcaaaaacg ccccgggaat ctgcttcgga gagcctgagg tacgcaaaaa agctcgtcgc
     3601 ggaatttggc agttgaggcg ccgcgaaccg caacacacag gaggccggac gcgatgctga
     3661 ccggcctcct gtttttttac ggcgggtgag atactccggc gatgttcaaa cccgcacgcg
     3721 cgccgctgcc acaatctgac ccgtttcggt ttccacggca cgaacatgaa ccacgcggtc
     3781 ggttccaaga cggtatattt tgccgaggaa cacgacgcgg ctgttcagaa ggtggcccag
     3841 ctcgattgcg cgggagggtt cgatcgctgc gctaacgctc aggctctgtt ccgcgatgat
     3901 ctgtgcgagg cggtcgcgtt ccaccatgcg aacggagccc agccgcaaca gggtttctgt
     3961 ggaaacgtcg cggagatacg cctcgaggtg ggaggaatca tcaagggtgc tttcgaacgg
     4021 caggagcacc gccgtaagcg tatcaccact gaatgccgca agctgcctct gaacctgttg
     4081 ccagagattc tccatccgca ccgcaaggtg gtcatgctgg tcaacgggcg gagggttgtc
     4141 ggtcacaagt gtcggtactt cggtctggta cgcgatgtcg cgcctcacga ggtcaacgac
     4201 gatgtcgtcc aatcgtcttg ctgaccagtt cgcgcccgtg tgatggctga cccggccggg
     4261 gtccgcagca ctgcccatcc cggagctttc ccctgtctcg ccgtaggtca gaaagatgaa
     4321 ctgaccgtac gttgccacgg aaatctgcct gaacgcgtgc tcaccgctct ggtccaggcc
     4381 gctcgcgccg accgtgttga tgcggatgcc catgctgttg gcggtgcggc tcgcccagag
     4441 gtaatcgtat tcctgcccgt aatccgtgtg cggaggtgcg tccgctatga ggaacgcgtt
     4501 gcggatgctt ccttcgcgcc aggcaaggtc cttcaacgtt gcccgcaatc ctgcgtggac
     4561 gtcctccggg gtatcacccc cgccgctcgc gctgaccgat tccagagcga gctgaaacga
     4621 atccacattg gcggtaaacg gcacgatgcg caacggttcg gcgtcccctc gatccctgta
     4681 caccacaagg ccaaagcgga tgtctgcggc tcgggctgcg ttctgcagac ggctgtgaat
     4741 tgagaacaac acgtcacgaa gctgctggat ttcgtctccc atgctgcccg ttgcgtccag
     4801 cacgaacgca acgtccaccg gaatctttcg cggggtccgg cgcggcgcgt ccagttgcac
     4861 cgtcacaaag ccatcgaggg aaggcctcca gggcacctgg atttcttgcc cgttggacag
     4921 gtggcatacg actgaagccg gtgtcgcgcg gtctgaccag aagacgcttg ggaaaaggac
     4981 cgtctcaccg ttcgcatacg tcacaccgcg ccacagtggg gcattgccgg cgtcccttac
     5041 cgtgtaagat tgcgccatca cgggagcgcc cgcggagtcg attacccgta gcacggttct
     5101 tccgtggtat gccgcgggaa ggaccatcgg aaggagcgat ttgcgtgacg agaacatcac
     5161 ccacgagggg tattcctcgt tatcatcgtg cgttcctgct tttacctgtg ctgcctgtgc
     5221 gccatcgaag gagagacccc tcacccggct ctccgtagca tgaatgtccg cggtgccgct
     5281 ggcgcgcgca gttccacgga tgccctcccc ctccgcaaga gccttcgttg cccgtccccc
     5341 gtactccgag tccgcggggg tttccacacc cgccggcgac ggaagttcag ccgggttttg
     5401 cgccgggggt gtcttctcca cggggctgca gccgaagaag acaagcgcca acgcggccga
     5461 taaatgcgga atgctgtgag aaatgcgcgg cttcatgcgt gaacctcctg tttcctgctg
     5521 cgggatatgc gtgcggtacc agaaacctct ttctttactg gtataccggc aaagtcggat
     5581 ttctattccc ggtcacgcca agttgcgtcc agttggttct ttcggggatg agggctacat
     5641 ttgccgtacg ttcttgattt tgcttgtcgc ctaaaggaga tgttccgtgc cacttgtctt
     5701 cggcattgtc ggttgtcgcc atggtcatgt tttcagtttg atcaaagaac ttctcagcgc
     5761 tccgaacacc gagtgtgccg gcgtttttga ctccgatcag gattcactgc gcgcggtaac
     5821 gagccgattt ccggtacgcg ccgcggcatc ggaaaccgag cttctcgata accccgatgt
     5881 tacgcttata gggacggcag agatcaaccg gaagaaggcg cccctgattc ttaacgcctt
     5941 gaacgccgga aaacacgtca tagccgacaa acccctttgc acttcactcc atgaactgaa
     6001 cgcaatcgaa cgcaccgcaa ccgaaaagcg ccttaagatc gggctcatgt tgacggagcg
     6061 gttcggcccg gccaaccgtc gcatgaagca gcttgtttcc gacgggttcg tcggccatgt
     6121 cgccaacgtg atgtgctggc ggccacatcg tctgggacga gcaacccggc cggactggat
     6181 gtttgtggac gacctgtacg gcggaatact tgtcgatctc gctgtacacg atgcggacat
     6241 catccggtgg cttaccggcg ggaattttct cgaagtcacc gcctatcagt ataacatggg
     6301 gaatcctgct gacaccgatt tcagtgacat tggtgtcatg cttggacgcc tttccacggg
     6361 tgcaaccggg tttgtgagga ccgactggtt caccccggca acctcgccgg tacacgggga
     6421 tacccgattt ctggtcaccg gcaccaccgg catgatagaa gtgaggactt ccggcgatct
     6481 gtggggcgga gaggccggaa aggagccgca ggtgctgttc atatctgaca cccagcccgc
     6541 cacgcgtttc gacggaattg gacccgaaag gccccttgtg catgatttcc tgaggagcat
     6601 tactgacggc aggcccgcag aaataaccac cgcggatgta ttcgaagcca cccgcaccac
     6661 gttaatggca cggttatcgt gcaaactggg acgcaccgta acccgttctg agtcgtatct
     6721 cgcttgaagg agagcacgcc atgaaactcg catgccaaga ggggctcgtt cccggaaaaa
     6781 gtatcgcgga gaagctcgcg aatcttcaga aggccgggta cgaagcgatg gaggtctggg
     6841 gcggaggcct tcctgaccgc gtcaaagaaa ttcgggcagc ctgcaagtca tctccggtga
     6901 agatatcgag cgtatgtgcc ggttacggag ggtgcccact tgattccgat cctgcgggga
     6961 ggcagaaggc aagcgatgac atcaaaagac ttctcgacgc gaccggcgaa attggcgccg
     7021 tcgggctgat ttttgtcccg attttcggcg gcccgcgcat cggtgacctc agcccctgga
     7081 aaaccgcggt ggaacttgaa aaggatctcc tggtcgagct gctttccggg tgggcggatc
     7141 acgccgcggc ggcaaagacg ctcgtcattc tggaaccctt gaacgggtat gaaacgcatc
     7201 tcatcaaaaa actggccgat ggggttgaca tctgcgaacg cgtgagcaag cccaacgggt
     7261 tgaagatgat ggcagacttt ttccacatgg gtctggaaga gccggacgtc gcggcttcca
     7321 tcaaagcggc cgggaaatgg ctttgccacg ttcatctttc cgaccacccg cgaattcttc
     7381 cgggatacgg acacagtgat ttcaaaaaag gattcgcagc gttgaagagc atcggttata
     7441 ccggctttat ggcactcgaa tgcgggtgcc ctgacgctga caagatgaaa gccatcacga
     7501 agacagcaaa attcctcaag gctcagatgt aactctgcgg cggattgtcg ccggaaggag
     7561 ttgtcatggc agtacgaatc ggttttatcg gctgcggcgg gattgcgaac caccacatgc
     7621 ggacgatggc gggcaacccc gatgcagaga tggtagcgtt cagtgatccg gatgtcgaac
     7681 gagcccgcgc ggcagcgaag cagtacggcg gcaaagcgta ctccagttac cgcacaatga
     7741 tttcgaaggg cggaatcgat gccgtgttca tgtgcgtgcc accgttcgcg cacacggatc
     7801 acgagaaact ggtcgcagaa gcaggactcg ctttcttcac ggagaagcca atcggactct
     7861 cgttgaagcg agtcgcagag aacgcagagc tgttcgcgaa aaagggtatc atcaccgcgg
     7921 tgggctataa ctggcgctac tcagacggcg ccgcgatgat gaagaagacg ctcgacggca
     7981 agaaactggc ggtggtgagc gggtactggg tgggcggcac ccccggtgta atgtggtggc
     8041 gacagatgaa gaagtccggg gggcaggttg tggagcaaac tacccacatc ttcgacatgt
     8101 gtcggtatgt cgcgggcgaa attgacgtgg tgttcgcgtc tggtatttcc gggttgacca
     8161 gtcaggatga gctcccgctg tatgacgttc acgatgcgag tgtcgttcag ttgcgctacc
     8221 ggtccggcgc ggtggggaac attacgtcgt cgtgcatcgt ttcacaaggc gtgggaaccc
     8281 aggtacgggc gtatgcccgc aatcttgtcg tcgtggtggg agaaggcagg tgccaggtca
     8341 gtatacccgg cgaagagcat atctacaaag acgcgcgaaa tcccaccgct cacgagcagg
     8401 aagcgttcct atgcgcggtg gcgaagaaaa acgggccgcc tattctttcc gactacgctg
     8461 acgcgatgaa aacgctgcgc gtcacgctgg cggccaacaa gtccatggag acgggaaaac
     8521 ccgtcaagct tgcatagaat ctcagatgtt cacgggggtg tccggcccta ccagcacccc
     8581 cgtgatctcc gtgcacattg gaacgcacgt gctgtttccc tatttttcta accgcccttc
     8641 aacgagcgag cctgcgtatc caaggttcac cttctcgatc ggcgagacta ctccctagca
     8701 acggattatc cgcgcatgaa gtttctgcgt tcgcagtcct ttccggtact gacaattctg
     8761 cttgccgctg ttgcctcgct gttgttcgaa gcctgttcca gcacaaagtc cgcggtcggt
     8821 ggcgggttat tgacggacgt cacgggcgag ttttcgagac gcgatacaat cgacattccg
     8881 ctttcaggcg tctccccggc gaccgttgcg tgctttccgg tgcacatatc cttttcgagc
     8941 agcgaggcac tgctcgctgg agaggcgaac ggactgcggg caaccgttct gctgaagttc
     9001 tcgccaatcg tgtcatggac ggaagaacgt tattacacgt tgaacgatca ggccgcgctg
     9061 gacacactta ccttcacgcc ggtcagcgtc gtaaccgatt cgtcaagtca actgcggctg
     9121 acgtattatg gccacgtcgc tgactcgtcg ttctgcgcgg tacagacacg gctggtggtt
     9181 tcgtcgtgga aggaagagga ttcggtgatg acggcgctcc ccgcaatgaa tgcgccgcct
     9241 ttttcgcctg tccgggtaga cacgacggcc tccgggtcgg tagtgtacct gcctttcgcc
     9301 cccgcttctc tgacgcagct tattgctgat tcggtatccc tggccgtgga aacgacctcc
     9361 ggcagcagca tggtaaggtt cctcagtcag gaaggcgcga caacgtacag cccgcaactc
     9421 accctctgga taaaagcgaa tgtgacttcg aagcggccgg aaatcgcggc tcatgacact
     9481 ctcataggcc tcaccctgcg gcctatccat gactgttatc gacttcagcg gctcgtgccg
     9541 gcgccagcgc cgtctgcagg atcgctccgg atatccgcgg gtgatgcgtg gcgcgggtac
     9601 gtccgcttcg acagattccc gatcccgggg gtgagcgaca gcgccggagt cgttcgcgct
     9661 cgcgcaacag cgaactcagc tcaactgcaa ctgatactcg ggccggacgg cggaggctca
     9721 ttcaaacggg acagcgccgc gatcctcatc tatccgctta ccgacccttt cgacccggac
     9781 agcgcccgcg ccggtctgct gaacttccgg acgggacgcc tttacggtgt cgggttcaat
     9841 acggcggaaa cgagagcggc gccggacgtg cgggtatttg aaattgctga cctgatcaac
     9901 cagtggtggg tcaacccatc gtccaacaac ggccttctca tctcgctttc gggagaggcc
     9961 cagctcgtgg acgctctgga aatccgttcg atgcggttga tcgtaacgac gaccatgcca
    10021 ccgcagttga tcgggaaacc ggcatccggg gaggcccccc gatgagatac cgtctccttg
    10081 tgccgcacct gctggtgttc gctgccgcgt gtcacgccag ttcgatcttc tcctaccacc
    10141 cccgtggcct cggttatgca gagtggaagg gcgacgcgcg gaccatgggg atgggtggtg
    10201 tgtctctcgc gaattcggga gaggacggtg ctaccagcgc gaatcctgca gggatcgccg
    10261 gttgtgaacg caccgcgctt tccgcgagct ttctcagtca gaagcggtcg gtgaaggatt
    10321 acctcggcta cgctggcaca ttttacgacc agtacccacg ttttttccgg gccgtggtgc
    10381 ctatcggccg gggcatagtc tcgtcgttcg ggctggaacc cgtcggggat gtccggatga
    10441 tctgggcgaa ccggaacaca gaaaacggcg tgagctatct tgattcgctt gaagcaagcg
    10501 gaggtgcgtg gtcatttggc gcgcaggtcg cccgcagttt cgggccgatt tcgcttggtc
    10561 tgaaagcgca ggttgtacgc ggcaacatga ctacagagtg gcgccggacg gtggtcgatt
    10621 ctgcggcacc actggcgact tccgcacttg cgatgcggcg tatctccgga gcggcgttca
    10681 gtctcggaag cgcatatcgg gtatctgaag agtgggcgat cggcggaagg ctggatttcc
    10741 cctcgaatct gagcgaaaca gacatcacat cgtacggcac gcgcattccc aaccagtatt
    10801 acaagtcgca tacggacgtg gtgatggcgg tcgcgtctga ccttgacgat acgacctcct
    10861 ccacggcgaa gttaccggtg ggatttggcg ccggcgtggc gtggaggccc ggggagcgtc
    10921 tgcttgtgtc attggacggt gagttccggc gctggtcgga cgttgaccct gcattccgaa
    10981 acacatggtg tgtggccggc ggcgtcgaat tccgcgtgag cgacgattac cggtcgtttc
    11041 tgctgtggca actcccttac cgggcaggag ttcggtggga acagcattat gtgccggctc
    11101 tcagttccca tggaggtgcc acgtatccag acgggaagta cctgactctg ggcgcaggga
    11161 taccactggg ccgaaaccgg ggacgattgg actactccgt cgaatacggc acgcggggaa
    11221 cgatttccac caatctggtc cgggaaaagc tatggcgaca caccatctcg gtggtcggct
    11281 gggagcgctg gtttgaacgc agcacacgtc gttagagggc gcggacccgg gatgcgcatt
    11341 gccacgggac tctactgcaa aaaggagtcc cgacgtcgtg ccagtggtgt cgcgtcgatg
    11401 gcactttccc aatttccctt cgcgaaatga agaccatgtt attgaggcgc accccgatac
    11461 gtcttctggt ttgcatcacg gtgctttccg tcatatccgc gtgcgggccg aagaggactc
    11521 atctgctgac ctgcgaggag tattaccagc gcggcatcaa tgctctggaa aagaagaaat
    11581 ggctcaaggc tcaggagaac ttcgagaaaa tcaccatgaa cttccccggg tgtgacctgg
    11641 tggatgacgc gcagtacatg ctgggtgaga cgtatttcag gcaggacaag ttcatcgaag
    11701 cgcagtttga gtttcgccgg gtggttgagg atttccggca atcggaccga acggaggacg
    11761 cgcagttcaa gatggccctt tgctcgcatc ggcaggcgct tccggcggca ctggatcaga
    11821 ccgcgacaca ggatgccatt taccgtttcc agcagtacct cgaggacaat ccttcagggc
    11881 gctgggcagg tgaagcgcgc cgctacatcc tggaaaacag gaagaaactt gcgcagaagg
    11941 atttcaacac cgcgcagttt tacgcccggc aggggtacaa tgagggcgcg ctgatttacc
    12001 tcgaccacgt catcgcggag tacccggaca ccggagaatg gctggaacga gcgaggtttt
    12061 tgaaagctac catcctcgcg caacgcgggc agacgcagga ggcgctcacc ctgttgcgtg
    12121 ccatcaacct cgatgcgctc cccggaaaag ttcagaaaaa ggttcgcgag acaaccgcac
    12181 gcatcgaaag cccccaagaa aactggtagc gtctgccata tgagcgaatc gaacgacgtt
    12241 gatacaccgt cccgtaaccg ccgcgtcggt ctcctcggcg gaacctttga tcctccccac
    12301 aacggccatc tcgctcttgc gttgtacgcg caggaacacg tgggcctcga tcagatctgg
    12361 ttcatcccgt ccgccgtgcc gcctcacaag atgcagaaaa caagcactcc cgctgccttg
    12421 cggtgggaga tgacgtgtgc tgccgtcaac ccgtacagcc cgcgactggt ggtttcggat
    12481 gtggagttga aacgtcctgg gccctcgtac acgtatcaga ctgtccaacg tatccggaga
    12541 atgctgggga acgaagctga gctgttctgg atcatcgggc gcgacaacat tgcggagatt
    12601 cctctctgga tggaaccgga gagaatcgtc gaagaagcga cagtcattgc aggagggcgc
    12661 cccggcgtgc ccctgccgga tgatatcccc gggtggttgg ccagacggct gatcgtgctc
    12721 cagggtcctg acctgccggt ttcgtcgacc gctatccgcg aacacgccag ccgcgggatc
    12781 atcaaccccg aatgggttcc cggcccggtg tccgagatca tacgacgaga ggggctgtac
    12841 gggtaccccc gcactgcccc tctgccagac tgaatcggtc ggttcccgcc cttaccagtt
    12901 gtgtatctgg tggcccgccc cgggaaacca catccaatcc acgaagaaac tcagcaatat
    12961 tcccagggcg tatcccgccg ccatcccgat aaacacaggc cgcccgcgtt cgtatgcctc
    13021 aacgcctccg atcttcagga tgatggtttt tatgagccac gcgaggaata tggcgaatac
    13081 ggtacggtga acgttgttcg ccgcggcaat agggaatcca atcgggtgca gcggccacgc
    13141 gggaaaccgg tattgcaggt aggtcagaat cgacatcacg agtgcgccgg caccgaggaa
    13201 cgtccagcgc cccgggtcaa ccgccacacg cgcctgcatt ttctggatga tcatgttgta
    13261 cacctgctca ccatgaaacg caaaatgcgt ggaccggaag gtcgttgctc cttcgttgta
    13321 cgcgataatc aggtagtaga acgagccaca cgcgtacgcc acgatgagcg cgacgatgag
    13381 aacggtcagc atgcggcggc ctctggcgcc gattttcgct ccgagccaac tgatgtggaa
    13441 aatcgcaggc atgaacgggg cacgaccatt cactacgagg gaatacgtgc cgaccattcc
    13501 tacgatttcc tgggccggga cacccactgt cccgaacgcg ccaaacgtga acatcgtagg
    13561 cgtgagcggg ctctgcatgt acacaaggcc cgtctgtgcg atcacccgtg tggtacccag
    13621 atacgccacg aatgccccga aaagaaacac aagcgccatt tcccaggaca ttccgagcga
    13681 catcatgaaa cccgtcatgt agacaactgc cacgaacagg ccgatcaccg cccaccgata
    13741 actcagcagt tcgttctcat catcagcttt gattttgccg ataactgcgt accacaccat
    13801 tttcaaatgg tttcgggcca tccacaggct gacaagcaca aaaacgatga agccgccgaa
    13861 gccttgccag cttgtcagcg cgtcacgcga ggaatacggg tcgtctgcgt cccccagagt
    13921 gtatccgaca gaacggaaaa agccgctttc cagaacgagt atgatgtccc agaaccagac
    13981 gctgaaaagc acgttggtgt tgacgagata cgcgaagccg agaacgtaca tgttcagttt
    14041 gatgccgaac gcggggaaat tcggcccgag ctgaatgctg atgccgtcca tcacaggcag
    14101 acgagggaac tgcggaagga agtagttcag acagttaaag aggatcacaa ataacggaat
    14161 tgccgctcca acccagaagg acctctttcg cagggtttcc gagatgcccc atttgccgga
    14221 tggacttgtg atgagttcca gaggtacctg aacaagcggg aacgcgagtc gttcgttatc
    14281 cgcccattgc tttcgtagca gcgaagcaag gcaaatcgcg acgagtgcaa ctgcgcctaa
    14341 aaaggttccc caccagaaca tcggtacgat ccagataccc cacgggatct tcgcgccatc
    14401 tggcataccg acaaatgccc agcggagttc gtcccgtggc ccctgcacaa ctgcccactg
    14461 cctgagatac ggccagagat cgctcatcca ccggttctcc ggtgttgcca cgtagtacgg
    14521 caccgacatg attcccagtg gccagttgag caggtttgac gtggcgacaa tcgatccgac
    14581 aaacgccatt gagaacacgg tgatgagttc cgcgggacga agcctcagca caggggctat
    14641 gcgaccgacc acggagttga ggagcacgac cccgaagaac aagcccatcg cgacgacggg
    14701 aagttgcgag agattgaggc gagatgcacc aaccagaaac tcggacaatg gcgcccagac
    14761 aactactacg atacatgcga gggtacccag gacgattgac cgcagggaga cgtcgtcgtg
    14821 gccatgattc ccaagactct gaatgcgcgc aaaatggtca gacatgtcga tctgttccag
    14881 atcagattgt tgagataaga gggcgatgcg aggcggattg ctcaccattc ggatgtcgga
    14941 taagaaaaag tatacgaata atgccccgcc aagtcacgta tttgtggtca attgcacgaa
    15001 ccaaccttca gggccccggc agccacccac tcactgattg cggctacttg tcttcatcac
    15061 ccgcacgcgt attatcttta accttcccgt gtggaagcac acgtgatcaa gctcgttcaa
    15121 tgatcaggcg ggagtttccc ggccgtcgga gcaaaccgaa tggcagcgcg ccagaaccgg
    15181 atgattctcg accagtgcga aatccctggt attcggagcc tcgccgttgc gcgccgctat
    15241 ggcgcgtaca ccgcgctggc caaagcactt actaccatga cgcctgacca ggtggtggag
    15301 cagatcacac gatccgggct gcagggccgc ggcggagcgt ggttcggaac cggtctgaaa
    15361 tggtcattca tgccaaaaga gccgcgcgtg gagagctttc tctgcgtaaa cgccgacgag
    15421 agcgagcccg gcacgtgcaa agaccgttat ctcattgaga agaacccaca cctcctgctt
    15481 gaaggtgtgc tgatcgcctg ctacgcgata cgagcgtcga ccgcattcat ctatgtccga
    15541 ggtgaaatgt gggagggaat ggaagtcctt gaaaccgcga tccgagaact ggtaaccgcg
    15601 aacctctgcg gtaagaacat tctgggcacc ggcttcacca tcaacgtgca cgtgcatccc
    15661 ggcgctggag catacatctg cggcgaggaa accgcgctga tggaatcgct ggaaggaaaa
    15721 cgaggctacc ctagaaacaa accaccattc cccgccgcct atggtgcgtt cggccatccc
    15781 acgactatca acaacatcgg aaccctggga tacgtaccgt ggataattaa tcacggagcc
    15841 gacgcgttca aaggcattgg cgaccccaat ttccctggaa ctcttatcta ccaggtcagc
    15901 gggcatgtca atcggcccgg tttgtacgag gccgaactga cggtaacgct gcgcgatctg
    15961 atcgacatgg ccggcggagt tcgctccgga caccggctca aagcagtgat tccgggcggc
    16021 acctccatgc cattgctcac cgcggacgat ctggacacgg tgatgtcgcc ggccagtttc
    16081 gcgccgcggg gatcattgaa aagtggcctg gggtcggccg cggtgattgt gctggacgat
    16141 acgtgcgaca tggttgatgt tgctctgaac atgatgcgat tctaccgcca cgaatcctgc
    16201 ggacaatgca cgccctgccg tgaaggaacg aaatgggtgt gcgacatgat cgaaagaatc
    16261 catcgccgtc gtgcgcgccc cggtgatctg gaggtgttgg agcaggttct ggtgaacgtc
    16321 ggagacatag atcggttcga gttcaacaag acgatctgcc tgttcggggt gtcctttgga
    16381 tggcccctca ccatgctgat ccgaagattc cgaagcgaat tcgaagcggc agttgcggag
    16441 agcacgccag cccctgtgcc gttgcccgtt ccagcgagtg tcttgccggc cggggacctg
    16501 cgggctccgt gaccaactcc gcaaaaggct ggtgctccgg gcgcaacaga aattgtgtat
    16561 gggaagtcgt agtctgccag cagcagatca gaggttctga atgccgacga tggtgattga
    16621 cggtcgggaa gtgacagccc cgccaggtgc caatctcata gaagctgcga agctggccgg
    16681 gattgagatc ccgcatttct gttaccatcc ggctttgaaa gttgtcgcga gttgccggat
    16741 gtgtttcgtg gaggtgacgc aagaggtgcg cggcaaagcg acaactcggc tcacaaccgc
    16801 gtgcaacacg cctgccgcag acggaatggt cgttcgaact gatactcaac ctgtgaaaaa
    16861 ggctcagaac gacattctgg aattcctgct gatcaaccac cctgtggatt gccccgtatg
    16921 cgacgaggcg ggtgaatgca aacttcagga ttatgcgttt gcctatggaa cagcctccag
    16981 caggtttgct gagcacaagc gcttccgcca tgccaagaaa ctgggttccg gtatccggct
    17041 ctacacaaat cgctgcattc tttgcgacag atgcgttcgt ttcctgaggg attttgtagg
    17101 aacaggcgag ctctcggtta ccgacatggg aaacggaaac gagatctccg tgtttcccgg
    17161 cagaccgatc gacaacgttc ttgcgggcaa tctgccggag atctgccctg tgggggcgct
    17221 catctccgag gattttctgt tcaaggcccg cgtgtggaac cttagcgcag ttgattctgt
    17281 ttgccctggc tgttcccgcg gttgcgcgac gtctctggac gtaaaggaca acgaagttca
    17341 acgcacccgg gcacgggaga accaggcggt gaacgggtac ttcatctgtg acgacggacg
    17401 ctacctgtac cacgtgatcc gtcacgagac gcgtctggaa cacccggcgg tgcgtgaagg
    17461 agaccagttg cgggagagca actgggatca cgcgctggat gtggttgtct cgatactggc
    17521 ggagttccct gagcgagtcg tgatactgct ttctcctttc ctgacccagg aagaagtccg
    17581 cgccggcgct gatctttctg aggttttgcg cggttcggtg ctggggcacg ttccaaacgc
    17641 tgttcaggaa gcgcctgttt tgttccccgg tgggttcgca atcgatggag acaaggctcc
    17701 gaattcaaca ctggtgaaaa acattgcgcc tgttccgctg caggaactcg cgataaccaa
    17761 agacaccgta gtgctctttt tcaattcctc ccttcgcacc gggtcagtgt cacctctgga
    17821 cgccgaaaaa ctgcggcgcg cagggtttgt tgcggtgatt gacgtcctgc ggaatgacat
    17881 caccgcggtc gctgatgttg tactgcccgg caggatgtgg gcggagaaat ctggcacctt
    17941 ttccaactca gccggtatgg ctcagtcttt cggggcggcg gtactgcctc ccccttttac
    18001 gcgcgatgaa tctgatgtca tcagagattt gacacggcga atcacgacac gcatttcaac
    18061 agggtgcgga gcgaaatcgt gatttctcag gaacagctca tcgtttcgct tgctctcggg
    18121 ctggtgctga tgctcgcgat gacggccggg gcaggggtgt tcacgttcta tgagcgcaaa
    18181 attgccgggt ggacgcaggg ccgctacggg ccgaacagag ctggtccgaa agggtacctg
    18241 cagttcctcg ccgacgggat caaattcgcg ctgaaagagg acgtgattca agactacgtc
    18301 gtcaaacctg tctacgtgat cgcgccaggg ctgatgatga tcccggcgct ggtcctgtat
    18361 gcggcccttc caatcggtaa gccctttgtg tggaacggca atgaagtgca ccttcaagtc
    18421 gttcaggcga acgttggtgt cctttattta cttgcggtcg ccagcatcgc agtgtacggt
    18481 atagtcttcg gtgcctgggc gtcgaacagc aggtacccgt ttttcggcgg agtccgggcg
    18541 tcggctcaga tgatcagtta cgagctcgcc atgggattgt ctatcatcag cgtggttctt
    18601 ctgacgagcg aacccggcat caacgcgttg agcatctcca acatcgtggc ggggcagacg
    18661 ggttactggt tcggattcat cccgcgttgg aatctgtttg caaaccccgt ggcgtttgtc
    18721 gtgttccttg tgtgcatgtt cgcggaaacg aaccgcctcc cgttcgacct cgctgaagcg
    18781 gaacaggaac tagttggtgg ctaccacacc gagtacagcg cgatgaaatt cgcgtcgttc
    18841 atgctttcgg aatacgtgaa catgatcacc atgtcgatgg tgatggtaac gctgttcgtc
    18901 ggagggtggc attttccggg cattgaggca atcggatcac aggtaataca ggtgttgttg
    18961 agcatcggag cctttggtat caaggtgctg ttcttctgtt tcctgttcct ctgggtccgt
    19021 tggactctcc cgagattccg gtacgatcag cttatgcacc tcgggtggaa agcacttctg
    19081 cccgtggcgc ttgcatccgc cctcggaacg gcaatcgcaa tcgctctgag ggtatgatag
    19141 cccgggcagc cggcccacag gaagaaaaca gcagcggcga ggcaatcaga tggccacgat
    19201 caatgtacaa cggcccgcgc ggttgactct ggcggagcgt ctgtatttgc ccgccattct
    19261 gaacggactc cgccttacct ggcgacatat ctggggcgag cgcgtcactc tccagtatcc
    19321 ggaggaagaa cgggaattca aacgagtgta ccggggtgtt cacagactaa acaaggatga
    19381 caaagggcgc gtgaaatgcg ttgcgtgcga gatgtgcgcg gcttcttgcc cggcacattg
    19441 tatcacgatc aaagccacgg aagtggagtg gcctgaaagg gacaagatac cgaaaatatt
    19501 tgagatagat gaactcaaat gcattttctg cgggtactgc gttgaagcgt gccccgagga
    19561 tgcgattcac atgacgaggc attatcacct cattgcgcgg tcacgcgaag acatgatttt
    19621 cggccttgat aagctgcttg cgatctacga cgagacgaag gaacaggaag ttctgcgtgg
    19681 agtggatttt ggagtgatac gtgcccgcat acctggaaag gaagcgggca atcccgacga
    19741 ggtgcagaag ctcgcctatc ctgtggtcgg cgtagtcacg aaagcggaga cgcaccacgc
    19801 acagtgaagg gacaaagaag gcggggcata tgtcgctggt gattttcgta gtgtttggct
    19861 gtgtagcagt cggttgcgcg ttggcgatgg tattcgcccg taaccccgtc tacggcgcaa
    19921 ttgccctcat tgtgtgtctt gcggcactcg ccggtgagta cctactcctt gacgcccctc
    19981 ttctcgcggc gttccagatc ctcgtttacg cgggcgcgat catggttctc tatttgttcg
    20041 tgatcatgct cctgaatgtg cggagggagg aaggatgccg gtggtgtacc gactggcgga
    20101 cgtatcttgg tttcgtgctc gcggggctta tcgggctctc ttttcttttc aaggcctcga
    20161 acgtcgcgca ccctgctcca ccggttggtt tccacgtgta tgatgtcaca ggcgtggcat
    20221 acaccatgtt cactgacccg acgcttttgt tcatcatcga ggcgctgggg gtgctgctcc
    20281 tgatgtcagt cgtcggcgca atctacctcg ggagaaggtt ctcggccgag gaggagctgc
    20341 tgatgaaaca tgaccgaccg gcaggatcga acagcacagg aacggccgga gacgctccct
    20401 gcggagaaac cgccccatga tccagaccat acacgttctc tggctcagca cgattctttt
    20461 cgtcattggg acggccggtg ttctgatccg ccgtaacgcc atagtgatgc tgatgtccgt
    20521 ggaactcatg ctgaacgccg tgaatctggc gctcatcggt tttgcccggc aattccgtga
    20581 cgttacggga caggtgtacg tgatcttcat aatgacagtg gcggccgcag aagtggccat
    20641 cggccttgcg ctttttgtcg ccgtgtttcg acacaagcgc acgctcgaca cgcagaagat
    20701 agacctctta cggtggtgat tacatcgccg tttctgttgc cgaacaacaa cttgttccct
    20761 gatagacccg cagggcacac gtgagaacac gaatatgacc accgcaatag cactgatacc
    20821 cttctggcct cttctgggct tcctgatcaa cattcttttc gggaagcgcc tgcggagcgc
    20881 aagcgcgtat cttgccatcg gcatgctggc tctgtcgttt ctcaccagcg ttgcagtatt
    20941 tacacggttt gtcggcgacc ccgcctcggt cctcgtccca cttttttcct ggatgtccgt
    21001 cggcgccctg aacgttgagg tcaacttcct ggttgatcag ctcacggtag tgatgctgct
    21061 tgtggtaacc ggcgtcggaa cgctcatcca cgtgtacagc gtggggtaca tgcacggcga
    21121 tgaacgctac agccgtttct tcgcctacat gaacctgttc gcggcgatga tgctggtgct
    21181 cgtcctcgcg gacaactacg tcgtcatgtt cgtcgggtgg gaaggcgtgg ggctgtgctc
    21241 atacctgctc atcggtttct ggttcgagcg tcctttcggg agcggcacca caaatgatgc
    21301 tgcccgcaag gcgttcatcg tcaatcgcgt cggggatttc ggatttctcc tcggggtgtt
    21361 tctcatctgg acggccgtag gcggagtgtc tttccagcaa acaagttcgg ccgtggaatc
    21421 gggatatgtc actcccggcg ttgccaccat tgccacgtta ttgcttttta tcggtgcaac
    21481 cggaaaatca gcccagatcc cgctgtacgt ctggttgccc gatgcaatgg ccggaccaac
    21541 tcctgtcagc gcactgatcc acgccgcgac aatggtgact gccggcgtct acatggtagc
    21601 ccgcagtcat ttcctgttcg atatcgctcc gttttcccag aacgtggtgc tctgggtggg
    21661 cgctcttacg gcactgtttg ccgcaagtat gggcgtgcag gagaacgata tcaagaagat
    21721 cctcgcatac tcgacgatca gccagcttgg ttacatgttc atgggcgtcg gcgcgggtgc
    21781 atacgccgcc ggggtgttcc acctcatgac gcacgcgttc ttcaaagcac tcctcttcct
    21841 tggtgcgggc gccgttatcc acgcgctaca cggggaacaa gacatcacca ggatgggcgg
    21901 gttgaggaac cgaatgcccc ttgtgcacgg aaccttcctt attggagccc tcgcgcttgc
    21961 gggtttcccc cttaccgccg gtttcttttc caaggactcg gttctcgcgg gagtgtacgc
    22021 tcacggcgat tttgccgtgt ggtgcatcgg tgttcttaca gcgggcgtga cggccttcta
    22081 cacattcagg ctgtacttcc ttgtgttttc cgggtcttcc cgcgatgaaa aggggcgtga
    22141 tctgcatgct ccggcatcat caatgacttc gattctgctg attctcgcga ttctcagtct
    22201 tgtgggcggc tacgtggagt tgcccttcca cattttggcc gcgttctcgg cgtttctgag
    22261 ccctgttgtc ggcatgagca gccacgaagg cattccagaa ctggccctca tgacggtagt
    22321 cttcctgttc gtcctcggcg gaataggcgt gtcgtgggcc tggtatggca gaagagagca
    22381 ggacgccacg gcgcttgcag cggagcccgc aaatcctttc ctgcgcacgc tgcgtcgcaa
    22441 atggtacgta gatgagatct acgaggtcct gttgatccgc ccgatacggt ggacttccag
    22501 aacggtgttg tggcgcgtga ttgacgaatg gataatccat gggttccttg tccagttctg
    22561 cggagggtat ctgtggaagg cgatagggtt tgtggtgagt ttctctcaca cgggccgcgt
    22621 cggcagttac gcctttggca tcgtggtcgg tgtgctggtg atcatttctc ttatcgcccg
    22681 ttaggcatgt agctcgcgga tacgagatac tcgcatggct ctgagcactc tcatccttct
    22741 tccggtgatc gccgcgctca ttctggggtt cctgcccgaa tcccgcaagg agctggtgcg
    22801 gaacgttgcg ctggtattcg cgctcatggt gttcgcgtat tcggtgggga tgtatttcca
    22861 gtttgatccc tccacccctc ggctccagtt cgtggaaaac aggtcctgga tcgacgcaat
    22921 cggtgcacgg taccaccttg gggttgacgg ggtaagcatc ttcctcgtcc tgttgacaac
    22981 acttctcacc ccgattgcaa tccttgcttc gtacgcggca attggcgaac gcgtgcgcat
    23041 gttcaacgcg atgctcctcc tgcttgaagc cggcactctg ggggtttttc tcgctcagga
    23101 tcttgtgctc ttctacgtgt tctgggaggt catgctggtg ccgatgtatt tcatcatcgg
    23161 catctgggga agctcggaaa ggctgaaagc gacgttcgtt ttcgtgttgt acacgatggc
    23221 aggctcggta ctgatgcttg tggcgatcgt cgcactggca attgtgaacg cacagaacgt
    23281 ggctgcggcg ctttcggcgc ccggcgcgta catggggtat gtatcgttcg acatctcctc
    23341 gatcagaacc gagcttctga caggcacgca gcagatctgg cttttcgccg catttttcct
    23401 tgccttcgcg atcaaggtgc cgctgttccc gcttcatacg tggttgccac ttgcgcacac
    23461 cgaggcaccc accgccggat cggtaatcct cgcgggagtg ctgctgaaaa caggtgggta
    23521 tgcaataatc cgtttctgcc tcgggctgtt tccagaagcc gcggtcgtct atgcaccgta
    23581 catcaacact ctggcagtgg taggtatcgt gtatggagcc ctcgtttcgc tcgtccagga
    23641 ggacctgaaa agactcgtcg cctattcctc ggtgagccac atggggttca ttcttctggg
    23701 ggtgttctcc ttcacccagg aaggaatgac gggtggaatc gtgcagatgg taaaccacgg
    23761 catttccacc ggcggtttgt tcctctgcgt cggcatgctg tatgaacggt cacacacccg
    23821 gaaacttgag gaactgggag ggacagcaac ggcgatgccg ctgttcgccg gtctttttct
    23881 tgttatcaca ctttccagcg cggcacttcc gggattgaac gggtttgtcg gcgaattcct
    23941 ggctctcgcc ggcgcatttt cgaacaaagc cacgcgctgg ttttctgtgg tcggagctac
    24001 cgggatgatc ctggcggcgg cgtacctcct ctggatgttc cagcaggcca tgtataccac
    24061 gaaacccggt gtatgggtga aacgcacgtg gggagacctc acggttcgtg aagtgctcgt
    24121 gcttttacct atcgttcttt ccgcagtggg aatcggggtg tatccggcac ctcttctgaa
    24181 accgatcgct gcatccagcc aactcgttct tcagaggatg aatcttcgtc cgaacggccc
    24241 tatccacgcc atcccgtccg gcgtggacga ccttgcgaca acctcccctg acctaccgga
    24301 ggcgagccga tgatctccgc ctccgacatc gccgttttgc tgcccgagat cgttctggtt
    24361 gcgggagcca tcatcctcct gatgctcgac gcgttctctc cgcggatcac gcgtagattc
    24421 ggcgcattgc tggcatccgt cgtgcttttc gcggcgctgc ttgccgttca gcagggcgaa
    24481 ccaagcaagt ttcccgcatt cggagcgctc gtcagcgacg cgttcacgga tatggtggcc
    24541 gtttccgtct tgttgttcgg cttcgtcgcg gtgatctcgg cgtggagtta tctcgaggag
    24601 cgaggactgg cgtcaggcga gttcatggcg gcaatgctgg ccacatccgc cggaatgatc
    24661 cttatctccc gctcaagcca tcttatggtg atattcgttg cgctggaaat cctgtccatc
    24721 ggcctgtaca tgctgattgg ctatgaccgg acagacgtct tgagcgctga agccggactc
    24781 aaatacttca tcctcggagc gtttgcgtcc gctcttttcc ttttcggcgc agtcctgctc
    24841 tacggcgtta ccggaacaat gcagcttaca ctggaacaac tcccatttca cacgagggcg
    24901 ggtcaggtcg gaattgccct gtgtattgcg ggactggggt tcaaaatctc ggccgccccg
    24961 tttcacctct ggactccgga cgtgtaccag ggagcaccca cgccggtaac ggcgtttcta
    25021 tcttcggctt cgaaaatcgc cgcttttgca gctctgcttc gagtagttct gccctccttt
    25081 tcgccatttg cggaaggatg gcacgccgtg tgggtagcgc tcgcggcgct taccatgttt
    25141 gtgggcaacg tcgcggcgct cgttcaggag aacgtaaaac gcatcctcgc atattcaagt
    25201 atcgctcacg tcgggttcat gctcatggcg cttgcagggg gcgcaaccgt tggccctgtg
    25261 gggattgaag ggcttctgtt ttatctcctt acgtacgccg tcagcgctgc aggatcgttc
    25321 gctgccgtgg ccgtaatccc gggttcgccg gacctccaca ctattcgcgg tctggcaaaa
    25381 tcccaccctt cgatagcagc gctcttcgcg ctgttccttc tctcccttgc cggggtacca
    25441 ccacttgttg gtttcctcgg caaactgtac gccttccgtt ctgctgtgga tgccggaatg
    25501 tacaagcttg cggcgttcgc agccttgaac accgccatgg gaatgtatta ttatctccgg
    25561 atcatcgtgc ggatgtacat ggagcaacgg gcagaaccgg cggcgtttgt tgtcccccaa
    25621 gtggcatacc cggtactcgt attggcagct cttgcagttg tgcttctcgg gctgtttccg
    25681 gacccggttc tggtgcttgt caggcaatcc gcggaagcag tggttccgct tgccagggcg
    25741 cccgttgcgt tctgaaagcc cgcgcggcgt tcgaccgatt ggtgaatcac gaagttgcgc
    25801 gttctcaaac cttttctttc gttcccgggg caaacccgta gtgagtagtg atcgcgcgca
    25861 aaacccccat gtgaggaaac ggcaaagtgc tcccttttaa cgaacagatg gacctaatcc
    25921 gccgcggtgt tgccgaaatc gtgtctgaag atgaactcgc gcaacgaatt tcggaagccg
    25981 tgcgaagtgg aaaaccctta cggatcaaat acggtattga cccttccggc aaggacgtac
    26041 atatcggtca catggtcccc gtaagcaaaa tgcgcgactt ccaggatgcg ggtcaccacg
    26101 gcgtgctcat catcggtgac tacactgccc agatcggaga tccaacagga cggaatgaat
    26161 cgcggccgtc tctgtcgaaa gaacaggtgc tcgcgaacgc ggccacctac gttgaccagt
    26221 tgatgaaggt gctcgaccct gaaaagaccg aggtgcgtta ccagtcagaa tggttcgaga
    26281 acttctctct gatccagatg atacaactgg ggcagcagtt caccatggct cagatgttga
    26341 gccacgagac gttcgcgaaa cggctcgaag aagggcttcc tctcgcaatg cacgagctga
    26401 tgtatccgat gctgatggca tacgactcgg tcgccgttca gtcagatgtg gagctcggag
    26461 gaatggaaca gcgcttcaat atccttcagg gcagggattt gcagcgcgcg atgggtcagc
    26521 gcccgcagat tgccgttctt tcccccatgt tacgtgggtt ggatggcggt ccgaagatgg
    26581 gaaaaagcct gaacaattat atcggggtga acgattcgcc agcagatatg ttcgggaaaa
    26641 tcatgtccat acgcgatgat ctgctcatcg attaccttac tctggcagcg ggagtgtcgc
    26701 aggacgcagt ccgcgcggcg gaggcgagac ttcggcgcgg cacgaacccg atggaaatca
    26761 aacgggaact cgggcgcgcg gttgtgcaac ggtaccacgg cgcgcaggca gctattgcgg
    26821 ctgaggaaga gttcctcggc gttttctcgc gtcgcgatca actgccggaa gacatgcccg
    26881 agttcactct ggacgagaac ccggtgcgca tcgtcgacat cctcgtgaaa acggaatccg
    26941 caccctccat gtcagaggcg cgccgtctca tacaacaggg tggcgtaacg ctcgacgggt
    27001 accgagtgac tgatgctaac gcggagatat ttgtcaaaga cggcgccatc ctcaaagtag
    27061 gcaaacgccg atacgcccgg ttgcggctca aagagtagca gcagccactc gaaaagtgaa
    27121 agagggcgcg gaaaactttc attccccgcc ctctttcact ctctcccggc gcaactcaca
    27181 cctggcgaat cagatggacg tccacccccc gtcagccatg atggcggcnn nnnnnnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cttcgcggcg cagtatgctg ccgttccggc
    27361 gagcgacaca actcccgcca cagacgatgt gtgaactatc gagcccccgc ctgctctgat
    27421 catttccgga atgacggcgc gcgagcaata gaaaacgcca ttcagggtca ctgcaagggt
    27481 tctgtcccac acctcgtccg tcatcgtctc caccactccg ccgacgctgt agaacgggtt
    27541 tgtgtgaagc acgtggatcg ctccgaactc gctcaccgtc cgagatacaa gtcccgcaac
    27601 ctgtcgggtg tcagacacat cacagtgaac gaagaggccc ctgttgccct cagatcgaat
    27661 ggtggccgct acctgctcac ctttttcgtc aactatgtcc ccaattacta cgttcgcccc
    27721 ttcgcgggcg aacagaatgg cagaggcgcg gccgattcca ctcgcgccgc ccgtcacgat
    27781 cgccgttttt ccttcaagtt gccccatatg cagctctctc acaggctgat agacttgtcc
    27841 gaatcaccct tcgtcagagt aacctatggg tttgtacccc tcgccggttt tcccggggcg
    27901 tttgtggcca taggcacgaa tgggtggagt ttcatacgat cgatacggga tggcaaggta
    27961 gggagtggat atacgggcac cgccctttgc gcatgattcc atcacggccg agatgacacc
    28021 tgtagtaatc gcggtgcgct cgactggata ctgcggcacg ccggtgataa acattcgttc
    28081 tatgtttcgc gacagatacc cgaaatggga aaatggcggc ccgtattcaa gcgcgaactc
    28141 cgtggcaacg accttcccat ccacgcgacc ggcaaatgcc cagtccacga tgtatccgtc
    28201 cagcatgatc acggtggccc gcaaaccact gaagtattcg atgacgaatg cgaaaggagc
    28261 cgcgcatcca gaccgtggcg gctcgtcctt ttttgtgaac acagtcttca gggcggcttc
    28321 aaaaaggtcc tgagaccaga caccgttttc tccggctttc cacacgtcat cgccgcggag
    28381 gcaggtaacc gccttcacac cggtctctcc accacgtcgg cgttccacca tgcactgcaa
    28441 tgtctcgacc gtgtggtatc catacgcttc cagcggcccg taccccagcg cgagggcgtc
    28501 cgtgagatcg cagtccagat ccagttccaa ctccggttcg cgccatccca ccggcagcga
    28561 tgatcccgcc ataagaggca cattcaactg ccttgcccgt tccagcatcc atgcggcgtc
    28621 gggccagttg tacgacaaat gcttgtccac gaacacgggg actgatttct tcgtttctgc
    28681 gaatactccg cagatctgct cgaacaatgc gcggcgcggg tacatctgac ggtcccattc
    28741 gtctcgcgga tagtcaccgt gttccccgac gagaagcacg ccatccacgg cgagatcgct
    28801 tccaccgagc gtcaacgctg ctcgaatgct ctggaacacc ggcacaccat gctcttttgc
    28861 tttcgccaga ccgatgtcgt ttggagcaat ctgatccagc cacatgctta caattctcgc
    28921 ctgcggcggt attactcctt cgtcagaggg gaacccctcg aggaaacggg ttacaatcac
    28981 atccgcgtgt gagttcggga aataggtagt aatgatcgcg gcgactctcg ggctttccgg
    29041 catggcagaa cctcctttag cgacatgtag aataggggga caattgcccc cggcgcaagt
    29101 cggtaccacc gcgggagtgg tgttttccgt ctgcacgcgg tacgtttcta ttggaggaaa
    29161 tgatgagttt tacggaaacg ctgtatcaca tattccccgc gctcggtatt ctggggctca
    29221 tcgcgctgtg gattctcgcg atgctgtgtg gcatgtcata tttctccgcg aaaatgcccg
    29281 acgcaccgtc gccaacgcga tggaagccag ccgtcgttcg atttgggatg gtcgcgttca
    29341 tggcgctcgt aggaatccta gttgcccagc gcaacggcgt atggccggac taccgtcttg
    29401 cagtcggtct tgcaggggca gtggcaggac ttctggtgga cttgttgtgg cgggtcttca
    29461 cgtcgcgcat gacgtgaatg agatgtgtgc ggtacggaat ggtcccgcgg agatagccaa
    29521 atcgcaactg gagatcggaa cgatgaagcg ttgttcttta gcgtggtgtt tgttggccgg
    29581 attggcggcg ttcacgggct gcgatagtac gaatccaccc caaaccggtc gaatacacgg
    29641 gagaattatc cccggtgcaa aagcacgtcc ggacacggga accgtgcagg tttccctgtt
    29701 tgtcgcgtgg gatacgacga aagggctcgc gactgaagcc gcaggagggc ccccgtcgtt
    29761 cgccagtgcg tacatccctc ccggaacgaa tgaatacgta attgagaaca tcacgttcgg
    29821 cacctacgcc gcgctcgcag catcctggcg cgacaccacc atgacgggag cggccgcgac
    29881 aaccacaata ggtgcgtacg gagctgaccc acaggctggt gacaatacgc cactctccat
    29941 cactctgagt ggaagccagc cggatctgga aatagacatc ccggtcgatt attcgcgcgc
    30001 caaagtctca accggaccta ccggcagcat ttccggaact ctcacactct ctggaacatg
    30061 gcccacaagg gccgtttatc tcgttgtgat gcctgcaaac aagatcggtc ctccccccgc
    30121 gtttgccgcg atgggtcaac cggtcaccat ggtcgcgctc ccgcaatttt ccggctccaa
    30181 agcctacaca atccctaacg taccttacgg acaagggtat gcggtcgccg cctacacgtt
    30241 cacccccccg atgacgctgg ggttcatcgg cgcgtatggt gtggatctgc ccgcggatgc
    30301 aacactcgac tcggttaccg tgggtaatgg cggtgcgata gatctgcgta acatcaattt
    30361 tgccatccgt ccctgacatt cctgccatgg ttcggttcac gaactcggta cgtggtcgct
    30421 tgatgctgcg ctcgggagta atcgtcttat tactctccgt ccgctttgta ggcgcagcgg
    30481 agatcgccgg aaaggtaaca aacgagcagg gtgccccgtt gccgtacgct ccggtgcagg
    30541 tgcgccagga ggagtcgtca tcgccggcgt ccggtgtcaa tgccgactca accggcgtgt
    30601 tcgtgatccg aaatgtgccc ccgggcaggt acctcatctc ggcggattac gtgggccacg
    30661 atccgtggcg tgagattatc gaactccggg aaccgctgcg aacggtggaa atcgtcctcc
    30721 gaaccaggcc gattcgcggg caggatatcg tggtgacggc agttcgccgc ctggaggcaa
    30781 cgaccgctgc gtcggtatcc gtatcgctgg taacggacga cgagttggag tatcgcggcg
    30841 ttcgctcact cgaccaggcg ctggaaacgc tggacgggtt gcaggtattt cggtcccaca
    30901 gtgttgcaac gaattcggtc agtatccgcg gctcttcgga cgttctgggt gggggcgtgg
    30961 ggaacagagt ccttctgctc gtcgacggcc gcccggcact taacaccgat tccggtgggc
    31021 aatcatggag tctcgttcca atcggtgccg tggagcgggt ggaggttgtg aaaggagcgt
    31081 tctccgcgct ctacggttcg tctgcaatgg gcggggtaat aaacctcata acccgcgatc
    31141 cgttgggaac accggagacg agactcgtcg ctcgttacgg tgccttcgag aagccacccg
    31201 cctggatgca ataccggtca ggcctcgcgg atttcgccga aacgtcggtg ctccatgccg
    31261 atcacagagg ttcgaccgga tggttggtgt atgccgccca caacaggtcg gacggtcacc
    31321 gcgaagcgtc ggattactcc gtctggcagg cctacgggaa ggtcgcgcac acggtctttc
    31381 ccggtctcga gatcggagga agcgtgggcg gcggcaggtc tcacgccgga tatccccatc
    31441 gatggaaaag cctgctggag ccgctgaaag tccctgatga gaagaaacag gaccggcaga
    31501 acaaagggtg gtggaatctg gacttgacaa caaaagccgg cactctcgaa cacgggctgt
    31561 ggaacgccgg cctgtacacc tacgggatgg agtccgccac acagcaaccg gttgccgggt
    31621 tgggcagttc cgtcacgtca caaagaatgg gcggccgact gcagtggcaa aagcagttgg
    31681 cgggttttct tacccagaca atcgggggag aaggttcact ggacttcgta cgctcagaca
    31741 gcgttctcta cgggaatcga tcggctcgca acgcggctct tttctccctc tccgaagttc
    31801 tttttgccac cggacagcgg ctcgatttcg gcattcgggg cgattacacc tcgcttccgg
    31861 gaaaggccac cgaaacattg gtcaatccga agctgggcct tacgatcccg cttgggaaaa
    31921 cgttctttgt tcgggcctcc gtcggaagag cctttcgatc tcccagtatc gctgagagat
    31981 atctcgtgtt ggaaccggca ggagggacag aattcgcgcc caacgattcg ttgcgcccgg
    32041 aaaagatgaa atcatacgaa ctcggtttga ctcacgacct ctcgccggtg ctgaaaatgg
    32101 acgtggctgc gttcgtgaac gattacgacg actggatcta ctggcgtgaa ctgcccgtgc
    32161 agaccgacgg gcgctaccga tttcaggtgg ccaatctact gaaagtgcga atgagcgggg
    32221 tggatgcgca ggcgacgctc gaaccgtttc aagggttctc gcttgcactc aattacctgt
    32281 atttgcgggc cgaagaccga acgccgggac gaagcaaccc cacgattccg taccgtccac
    32341 gccacacgtt ctcgggtgtc gtggtgttca cccacggaag gttgcgaaac acggtggttg
    32401 ttcgaggaag gagccgcgtg gaagaagagg tcttcgccgc ataccaggtt gatgctccgc
    32461 gcggcttcgt gctgacaaac ctgcgaaccg actactcact gcgccgctgg ctcacctttt
    32521 cgttggaagc aaacaacgtg ttcaacgtgc agtatgagga gatggctcgc ttcagaatgc
    32581 cgggacgctc cttctcggtc ggtctggcga tgaaacggta gcgctccgtt ctttgacggg
    32641 tctcagttcg ggccgtcgtt gaacaggctt ctgccacgaa gagactacga agtcgaacgt
    32701 gccgcaaaat gcagttactg ccgtttcttt ccggagatgt gcagaggtgc agctgaacgc
    32761 gtcgcgcggg tgaaagacac ctgaatcgtc cgaatgactc caccggacaa acaaaccgcg
    32821 gagaggctgt cctgattctg cctctccgcg gcgcaggttg ttcgagactc agtgctccaa
    32881 ctgccggcgg tgcgcctcaa tatcgtaccc attctcccgc atgcttttga taaacgtctg
    32941 ctctttgttg agcacatcgt ctattgccgc cggcatgtcg tcaagcacct ccgccggaat
    33001 gctgacgaca ccgttcatat cggcgtggag aatgtcgccc ggattgacca cgagcccgcc
    33061 aacctccacc gggatgtcag ctttgccaac gtcaatccag ccgtgagaga caacgacata
    33121 ttcgctccac gtcttcatcc ccattgcttc aagggcttca aggtcccgga ctgcgccgtc
    33181 agtgatgatt gctcgcgcgc caagaacctg tacctgcgtg cctacgatct cgccccagtg
    33241 agatgaccat cctttgcggt gacatgtgtc cttcaccaca cagatggccg gcttggggga
    33301 gttgtgaatc gactccagcc atttgaaccg ttccctggga tccgggggga agttctcgtc
    33361 gtactcgact attgtgcagg tcgtggcata ccccacgata cgccccatat gggggaaccg
    33421 gcacacaatc tggggcccca tgtatccctc gcgacgggac cgtaccttga accgctcaat
    33481 cgcattggaa atggtcgggc tgtccaccga ttccagcttg cgtatcagtt ccggcgtgag
    33541 ctgacctttt gccattatgc aactcctttc aacggacgtt ggggatattc aggaagcgag
    33601 cacctcatct cccacttcgg caatccattc tctggtccat tcaaccgctt caaaatcctg
    33661 gtcgagcagg taattcgtca ggaacatcat gctatcgatt cttccacttt tcaaccactc
    33721 gagtcctagc ctgcactgat gttgcataag gtcgagtggc attggcgccc gatctccgta
    33781 atcccagaga tagatgccga gcgagcgccg tttgtcagga acgagccgtt caagtttctc
    33841 cagattcgtt tccaggtgag gcaattcccg tccccaccac tcccagaaac tcacgacgtc
    33901 gcacatattc aggtgttcga tcacgggtag atcgagctgg tgtcggtaca gcactgccca
    33961 gagatccagc gttcggtgag ggagaaccag tttttctcga atccgcgtga gtttctcgat
    34021 cgaatggacg gcgtacccgt cctccggctt cccgttgaag aagtcatcca tgaaaatgcc
    34081 ggtgatattc gggaaacggc atgcaaggtc gcacagcatg tcgagttcct tcccctgtgc
    34141 ccatccggtg acgccgccgg aatcaacgat ggaccagacg acctccttca gggggcgcat
    34201 tattcggagg taaggctcaa ggggcgggta agggccttcg cgtgatacga tcatggcgag
    34261 gcgctcgaca ccgaggtagt acgcaccttc caccggggtc acacgggtaa tcgggatgtt
    34321 gtaataggtg tcgtcacatg cctgctcgac ggcaaatatc cagagtttgt ccttcacccg
    34381 taccactctg acactccctc gcgtgtgttc ggcaacgcgc ctgttctccg gaaagaacat
    34441 ggcagacgga cttgtgggaa atggtactcg ccctctgaag aaaggggcaa gccaacgcga
    34501 aagccgcgag gcatggacac aatgagccct cgcgccgtaa actacaaagg agcgtgtcaa
    34561 gcgtctgccc gatcgttgcc atggaggact caatgccgat tgagttgtgg gatcgcgcgc
    34621 agtggcagga gttttctgcg gcccgtccat cgcctttttt cgcccagacc cagtggttcg
    34681 agccgacgtg ccggcttatg aatgccgaac ccatgtatct cgtgctaatg tcgaggagca
    34741 acccggcttt ctgtgtctct ctggcaagga agaaacggtt ggggcagacc cttttgctga
    34801 ccccggcaat tgcgccgtat tccgcatgga tcgaaaattt ccccgacgac ctcgcaccgg
    34861 agcggcgaga agcccggcgg ttggaagcga tgggggagtt tgccgcgtgg tgcgaacaac
    34921 acgccgcata ctgcaggatc gttttgccgc cacaggtcgt cgacgtccga ccattcctgt
    34981 ggcggggctg gcgcacagaa gtgaggtaca cgtataagat cccgcttgag cacggcgtgc
    35041 accccgtttt tcgacagaat gtgaggagga atcttcgcga cgcacgcgag gcgaatctca
    35101 gaacggagtg tctcaccggc gaacatgcgc acggagcatt gagggaaaca gtggcagcaa
    35161 cattccggcg gcagggagaa gcccctcccc ttcccgcaca agcctgggac gactacctcg
    35221 cactcctgtc ggtgtgcaac aatgttgcca cgatgggggt tttccgcggc gatgcagttc
    35281 ttgccaccat cgcaatcggg ttcgacccgc ccactgccac cggggccggc caatcagcgt
    35341 atgaactcat cgcgggaact actgacgaag gcatacctgt gggagccagc gcgatggccc
    35401 tctgcagtgc gtttgaattc gcttccggga aagtgggcga gttcgacttc gccggagcga
    35461 acatggagac gattgcgcat tacaaacgcg gtttcggggg aaccctgaca ccctacctcg
    35521 ccgtggaatg gtcgcggtca cgccgggctt cttttgttgc gaaaaccgca cccgcatgga
    35581 agcgcaagtt ccgtacattg ctcccaaagg ggtcgagatg aacgcgcgta acggcggcgg
    35641 catcgcagtc gacatggttg tgtacaacga cctcaggcac gacgcgcgtg tcttcaagga
    35701 ggcaacgtcg ctcatcgccg ccggctacaa cgtccgcatc atcgggatgg aaacaccgag
    35761 caccccgccg ttgaccggct gggaagaggt aactgtcaaa agaatcccgg tgggaacatc
    35821 ggactccctg cgcatcaggt acgcgtgttt ctggcggcgc gcgttctcag cgttgatgga
    35881 gcgcagagcc cggatcattc attctcatga ccttgacacg ctgtttccag cctggttggc
    35941 ggcatcgctg acgcgcgcac agctcgtgca cgacgcccat gaactctggg ttgagcttcc
    36001 cagtctcgtg ggcagaccag tcgttcgcgc cgcgtggcag ggtttcgcaa aaacgctgat
    36061 cccccgaagt gacgccgtga taacggtgtg cgagggtatt gcggacgaac tgcgagcccg
    36121 atatggcgtc ggcgcggtcg tgctgcgcaa tctgccaaag gagatcggtc ccgtcgaacc
    36181 ggcaccaatt cgcgagatga ctggctgtcc cccttcgcgt gtgcttgttc tctaccaggg
    36241 tggtttcctt ccggggctgg gcatagaacg ggcgattgac gccatgacgc ttcttccgga
    36301 cgcccacctt gttctcatag gtgcggggcc gctgagaaaa gacatggagc accgcgccgc
    36361 cgcgtcgccg gcgagggagc ggatcagttt tcttgatgcc gttccgttca aggatttgcc
    36421 tccgtacacg gctgcgtgcg acatcgggct gttccttggc gaatccgccg gaatgaacct
    36481 gcagcttgct ctgcccaaca agctgttcga gtacatcgcg gcgggagtcc ctgtggtggc
    36541 gaccggttgg ccggaaattc gacgggtagt tcgcgagtac gacgtcggtg tactggttga
    36601 gccgggatgc gaccttgagg cggttgcgaa cgcgatacgg agggccgtct ccgacagaaa
    36661 acgattgtcc gcaaactgcg tgaaagcggc gcgcgagctt acgtgggagc gcgaatccga
    36721 ccgtttgctt gaggtatatg cgaggctttc cgcccgcaac tcctccccgg agaacaggaa
    36781 cgatggctga gataaccgtt ctcatgccaa cctacaaccg gccggagcat ctttccgcag
    36841 cactagaaac actcgccggg caattcgaga gagaagtgcc gttttcggtc gttgtggtcg
    36901 atgacgggtc ggttccagca gttgagcttg atcccggagc gttcccgttc cgactgattt
    36961 tcctccgact ggagaagaac tgcgggcgtt ccgccgcgag aaatgccggg ctggctctgg
    37021 tggacagccc gctggtggtt tttctcgatg atgacatgag ggtccttccg ggtttcatac
    37081 gcgcttacca ggaggcaatt gatcccgcgg ggcgggcggt cgggttggga agcgtctgtt
    37141 tccaccccga cattccgcgc gacgcactga cactttacct cgaaacgcgc ggcatcgcaa
    37201 aactgaaagc cgatgaaccg atcccattca ggtattttct gacctacaac tccgccgtgc
    37261 cgacgtttct tttgcgagaa gcggggggct tcagtgaacg agtgcgcgcc tggggaggtg
    37321 aagacataga actggcgtgg cggttgcaga aacacggagc gcggttcgtc cgtgtgccgc
    37381 aggcgcgcgc gtatcacgct cacaggcggc gcctggagga tgtgctttcc ctgtccgaat
    37441 cgttcgccag ggagtgcatg cctgttcttt ttgacgaaga ccctgttttc acgacgcagc
    37501 tccgcgcaga tctgctcggt ccggttcgct acgcaaaaag gaaccgttgg aagcgcagcg
    37561 cgatccggtt tgtcacgacg ccatgtgttt cgcgtcgtgt ggaagcactc gtgaagcgac
    37621 acctcggcgc gcgctggccg atgtgggttt tcgactatct gatagcgtcg gcatggcggc
    37681 gggggctgga tcaagcccac gcccgaggcg aggaacgaga atgactgcat cgcggttgcg
    37741 tctcctcact gtgatggcgc tgctgttctg cgggtgcgga ggataccagc tcgaatctgg
    37801 tctcagcgca ctcgcgccga cgcgagcggt gcgccttgtt ttcatgcgct cgttcggcga
    37861 acgcggttcg tcatccgggt gcttccgtca accgcggggc atcgcgtgcg cgtcattcgg
    37921 gcactggtac gtcgctgata cagggaacga ccgcatacag gagttcgacg gcgaggggcg
    37981 ttacgttcgt gagattggcg ggttcgggtg gagcgacggc cagttcaacc gcccggcagg
    38041 catcgcagcg ggccgcaatc tggatatctg ggtggccgac acgtataacc ggcgcctggt
    38101 gaggttgggc caggacctcg tctgggcggg cgtgatcaac aaggacgcgg aaggcggccc
    38161 gctcgggtat ccgaacggta tcgcgctcaa cgttgatgcc agtatctggt gcaccgacat
    38221 cgatgcagac cgacttatcc agctctacca ggaaaccccc tcagaattgg gagcgcggat
    38281 ccagtcaacc ggcatactct ccgaccccgc cggggtggcg tgcagccccg acgggaacat
    38341 cgtggttgcc gataccggga acgacaggat cgcagtgttt gaccgcttcg ggacgcttct
    38401 gcgcacctgg ggagagggga ttcttctgcg tccccgcggc gtgaacgtga ctccacaggg
    38461 ggacatattg attgcggaca ccgggaacaa ccggctcgtt ttcctcaacc ggatgtatca
    38521 ccttacaggt gaacatgggt tgcgcggaaa cgaagatggc gagtttctgg agccctgcag
    38581 cgtggcaagt gaccctgccg gcaacgttgt gtgggtggcc gatacgggaa acaaccgggt
    38641 gcaggttttc cgtgtcatcc gaaccaccga atagagcctt tgaagccgct tcgtcagagt
    38701 tcgtatttac gtgtgcgcct ttcggagcat gccttcgtag acggccgccc cttcccggaa
    38761 aaggttcaga acggtggtgc gctgctgctt gttgtggaag cgccctttcg tgttgaccca
    38821 gtcaactgag ccacggatca catctgcaaa gaaccgggcg gcttcttccc gtgccgggga
    38881 aagggcgccg gtctggacgt agaccgggct cgtatgcgcg aagatggcct gatcgaagct
    38941 gtcg
//
LOCUS       DAOSOO010000006        38917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1084_length_38917_cov_5.645824, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000006 DAOSOO010000000
VERSION     DAOSOO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 38917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38917
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_38917_cov_5.645824"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT86_00905"
     CDS             complement(<1..221)
                     /locus_tag="PLT86_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT35208.1"
                     /translation="MQKQLLGLILAGGRGTRFGRLSERVPKPLLPAAGKPLIVHNIDG
                     LRRAGITEITIIISPDSNQVRSVLGNGHA"
     gene            complement(258..2768)
                     /locus_tag="PLT86_00910"
     CDS             complement(258..2768)
                     /locus_tag="PLT86_00910"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT35209.1"
                     /translation="MAEKNQWSASITFSDATTADGISLRQGVGRKTKPITDNRTVVPA
                     HVSGRACARAVPSGKKGSVGGYWRGTSESVDVQDGVFWLQIESPPPHSLREVYVTIDF
                     FESELGFIAVQYHRNGLEGFVHHVCPPGASKPWDSTWVNTLDTGSWQRATIRLVEPVF
                     DHSGFHDADLKIIGSGPLVLSTVRVSEERPEDFSDTDQMAHARRAEEHLSQSYPRASI
                     PVSIGNIGAIFEERDDSGVLSVPPWLPLYKGMGVTALQSYVRWSSIEREEGVWDWTFY
                     DWVVAEAKKFGLKWVAFIMIGPWYAMPKWWLATSNNYRNKCLEHGQESWVQSIWSPVQ
                     LDYVERFMRKFAEHYPEELIESIMVGISGDFGESTTNGVFTGDMYHTHVGYWCGEDVA
                     VADFRRTLREKYGSIGRLNEAWASSFGSFDEIRPVVRKDAPSDRAWIDQINWYCGQMT
                     WWMQRWGEICHEAMPNTVFYNAAGGAGDPPRAAHWTDQTRALKHTGMGHRATNEGCDY
                     AFNYVYTAWQGVGCRFYGLPFGNEPWGGDMAGNGVLGRVFNLVTQHGTNFWSYEGHLH
                     SPSGRLALQRGLPFLNGEYRRVNRVAVFYPWTHFVLADDHGFSEQGMRDRFWPQAEEL
                     RDIIDYDLIDDGLIKDGIMKDYDFLVILQGSTYEGPELERLYRWVENGGVLITHNLGV
                     PSTVEGDLSLGLRLMAFEPTVSPLEKELGGRMCRTGKGCVVMLPVCANRKGYFGDDRW
                     ADDHKDHPATHPAFWEMLTKTLANASKLDVGVEDYAIFDGVRDEVYGALMERAGVRGV
                     MFFSQVNSDVTKTVRLPGGRSKNVIVPAGQMVFAPFEG"
     gene            complement(3138..3803)
                     /locus_tag="PLT86_00915"
     CDS             complement(3138..3803)
                     /locus_tag="PLT86_00915"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT35210.1"
                     /translation="MCNEKDCCEDQPVGLVRINEPAPEFEAVTTHGVLKLSDFKGKWV
                     VLFSHPADFTPVCTTEFMAFANHQEEFDKRGVQLIGLSIDSVHSHIAWVRSIESLFGV
                     KVKFPVIADLDMKVANAFGLIHPKASTTSAVRAVFVMDPDQLVKAIIYYPLTTGRSIP
                     EIIRLIDALQINAAKKVATPEGWKPGDKVIVPPPATTQAAEERMKATDMEVVDWYFSK
                     KSV"
     gene            4138..4401
                     /locus_tag="PLT86_00920"
     CDS             4138..4401
                     /locus_tag="PLT86_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35211.1"
                     /translation="MDDIRFNARERLGEKRRAFVCRLTDGRVAIIYDGAGEDEPPATV
                     NDLDQAKRQVALLVPDIRVPLYEEHELKLVKQKLAGISSLSPV"
     gene            complement(4438..5220)
                     /locus_tag="PLT86_00925"
     CDS             complement(4438..5220)
                     /locus_tag="PLT86_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35212.1"
                     /translation="MNTFRWMLLAGTIVVLVAAAPRIRPPEQPESVPLVHDPVRRLAS
                     RFDTFASVPGDPRGRIVYGTSRGYLHLLELTGSRYQEIWVSPPLLSRIQKVLVADLGN
                     DGTFQIVATNTRGGLYVYDVNTFALLSRTQETQFRSIEAMTVADVDGDRRMEILFLSE
                     NRLFIFDAVHFVEKWRSDNLYRATDIAVGDVDGDGQQEIVLSSGHVLDAAFRNLEWEN
                     TTPFGEIIELGDVDGDGKLEVIGGTTQATTIWDVDERREKWD"
     gene            complement(5233..6576)
                     /gene="larC"
                     /locus_tag="PLT86_00930"
     CDS             complement(5233..6576)
                     /gene="larC"
                     /locus_tag="PLT86_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOT35213.1"
                     /translation="MRIAFFDCFAGIAGDMTVAALLDAGLDFEYLQAELRKLRVEGYE
                     LRKSTTRRHHIAATRFDVVIHGQVPYHTHATSADSLQHTRHEHPHDHGPDDHHTHGAG
                     RGHHNLDEGGPSSEERDHPVPLAAHVHRRLSDVVELIQTARLSQGVEKNAISVFKRLA
                     EAEASVHGTDPEEVHFHEVGAVDAIVDVVGTCLGIETMGIEEVHASAIRTGIGTIRAA
                     HGVMPVPAPGTLELLRGVPVDRTSLPYELVTPTGAAIITTFAKSFACPPVFVPETIGY
                     GAGSRDTQEIANLLRVEIGRTTGSIGEERALVVETTIDDMTPEVYGYVLERLFEAGAK
                     DVFLSPVIMKKNRPGIVVHVLVHPEQRERVISVLFGETSTLGVRISDVVRRVLPRRSE
                     TVETPWGPVRVKCAEWDGRTRITPEYDDCAAIARARSVPVLEVYEFVRKAADKSR"
     gene            complement(6603..6989)
                     /locus_tag="PLT86_00935"
     CDS             complement(6603..6989)
                     /locus_tag="PLT86_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35214.1"
                     /translation="MVSGDDPRSYRHPSNAHEGQANSRVLPPAGKWLAGRLKVCTKQA
                     VTVVTPEVRASSELPSFDPATGVPRASEGTDWVPLTEARSGREISSVVITAGPWGKKR
                     LARVIRTWEVLFNARAVIPETIECPG"
     gene            complement(7003..9087)
                     /locus_tag="PLT86_00940"
     CDS             complement(7003..9087)
                     /locus_tag="PLT86_00940"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOT35215.1"
                     /translation="MPIALGLDAGGTYTDAVLYDLSEDRILASAKAPTTPPSYIEGVA
                     GAIAGLPAAIRSRATYAALSTTLATNAIVEGRHAPAGLVLIGYDGFEAREVRWQPKRV
                     VAGRHTIRGVESEPLDETGLRVALTELLEEGVSGIAISSVLSVRNPEHELRAAEIAHE
                     LTELPIVCGHEVAGVLNALVRAETAALNAGLIPIVQQFLHAVEGVLSEVGLSAIPCFM
                     VTGDGSLMATRVARLRPVETILSGPACSALGAARLSGVAEGIVVDVGGTTTDIAVLRG
                     GRPSMAPGGVSVGEWRAGVRSADVCTRGLGGDSSVAVQHGKLSVGPQRVIPVSYAGHK
                     CAALREEYRELLERVNSDGSPGGDWRLTNPTDAFLRLQSEARSALEDQEKTILDALEN
                     GPKTRWGLAKAVGYPHQSLLRTERLERWRLIQRVGLTPTDIWHVTGQFQPWDKESARI
                     ATELAAARLGVSAEEVVARVGEIATQMLTRQVMHAYFGVPDEGNHRGAHTLESHLTRI
                     VLREVPRNGLGASLNLRPPIIGVGAPAHLFLSSVSDRLGGTFVLPEGAPVANAVGAAT
                     GAVVATATALLRPEENGSIVCYTPEKRISTADRERAIAVARDVLVPLVQEELRKKGAV
                     AASVTVRVEHFGAEVKDVPDPIWWESTVTVRGMGHPFGADGNRDDGLPDVVFQEEWVT
                     PGLKEAEQRSTV"
     gene            9285..10493
                     /locus_tag="PLT86_00945"
     CDS             9285..10493
                     /locus_tag="PLT86_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35216.1"
                     /translation="MVAKAQLDPSPFGMGIYPCHRTSGETTLRALGLARDAGIKWTRD
                     ELGWGGIQPERGVWKWEAFDRAIRLTREHGIHTLGLLCYSAPWAATHRTAEGKPSVVS
                     LPDLSAWKEYVFRTVERYRETIHVWQMWNEPNISGFWQPKPNPREYAKLLVAGAEAAR
                     AADPTCWIVGCNTSMVDLAFDRVVFEEGGWDTTDIIGVHPYRYPHAPEHTDMLGELRN
                     LAALAYEFGAVKPIWISEIGYATHLGPGGSSEWWSAAMLMRVYLTCWASGLVQKVFWY
                     DYRDDGTDPTYNESNFGILHHDWSPKMPYHGFKAMAHALEGFSPEGRVDLGEGPYVLK
                     FVRGDEVRYAAWTVGKNARVPVPAPADQVTVIRAWDAPLQAEARNGWVSVALDAAPVF
                     LVDSRRWTTS"
     gene            complement(10483..12807)
                     /locus_tag="PLT86_00950"
     CDS             complement(10483..12807)
                     /locus_tag="PLT86_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOT35217.1"
                     /translation="MPRRPALYPRQKWEWAKDLPVSDAPWEDLLRLPDIRFLTDNQVS
                     LWTSPDSIGLEKVWFYPVPRAPHLEVERFDSGRIAQSSVHVLRGPRGALMAGGKPVTW
                     DTQNLQWSPAEITRTFVSPEVTLQETVTIVEDAIVVWLRPVEGTSELPLSAAWSSSVE
                     RTLSGSWRMHDGARVCMHDDGIAHGWVWFGSEVTNWQGKAASGKFGWEASIPPQGGVL
                     VLLLGYDQAAVERRLEAMIGQCSNRPTADIAELFLTKARDAWQWYMKRMVPGLKGAEE
                     WVRRLYYYQTALHKINLYDIPYEPFVYPYTCPWKTGAVWQWSWNTPMNSIAERWLNDP
                     AWAESGIELLRENGGALNIGASLHRLRKPRQFRDVNEYLPALQDAIGGVKNLPATARQ
                     FDWAFIMPHTTPLGVHGIWEVFRRTGDEDYLRRQLPEMVAYEARLTSHDADGDGLVEY
                     EGMVDEYDYSLRWRTAVNGYKKGTESLIRFDRPLELIDINAQLCLLREDIIEAAGIVG
                     DRNLAKRIRQRLNKTARAINDLMWDETRGWYGDLDAVTHELTGVCSVAAFSALMSGVA
                     PADRAERMVRLLQDPAAFGTPYPVPSVAADTPDLDPGHITYGGDVLITSGVWLTVVAL
                     VRYGFLEEARRIIWKALRMLGENAPTSSYSYNSMTGAPNMDPHTFCSQSAITLDLFVR
                     FVVGFVPRYDDIIECWPFALPTEWEHLEFGPMTWRGDVDVTVVWDSKSGYTVRAGRSE
                     FTAREPRHLWLALDQRDRLVELPDPSVSGMPVMT"
     gene            complement(12976..13995)
                     /locus_tag="PLT86_00955"
     CDS             complement(12976..13995)
                     /locus_tag="PLT86_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35218.1"
                     /translation="MDAQSLAKTVQTPFKHGSLILSPSFRPGDFDSHGVDCPFVFRHE
                     DRFLMTYIGFDGLGYRTGLAWSDDLLVWHREGMILDRGIAGSVTQHNAALTWILRDND
                     LFGTGCLRQIDGRYVGTYHAYPAQGYESGPAVIGICSSTNLRDWEIGEPCLIPAEGEE
                     WERGGLYKSCLIEFQRRYYLFYNAKTVGVPWIEQTGVAISRDLMRWERYGGNPILRTG
                     AEGAFDERFASDPCVLRVGDAWAMFYFGLGRDGHARDGVAFSRDLLHWEKSGSVLVDV
                     GPPGAVDSRYAHKPSMFWHRNRLFHFYCAVSPRSEQAAVGAVHTSETRGISVAVSSNG
                     SRSLL"
     gene            complement(14020..14403)
                     /locus_tag="PLT86_00960"
     CDS             complement(14020..14403)
                     /locus_tag="PLT86_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35219.1"
                     /translation="MPLTLHSTDERTEVQVRVDDSAMVDPLQLRRDVIAALQRSARKE
                     ITLSLEGVAKIDTRLLSAIKMAVTEARIRRRRIILVSAPAAIRFALSGTSFRVRWAAG
                     LFPLDTESPAGALILNRYTSRVSAV"
     gene            complement(14429..15070)
                     /gene="lexA"
                     /locus_tag="PLT86_00965"
     CDS             complement(14429..15070)
                     /gene="lexA"
                     /locus_tag="PLT86_00965"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT35220.1"
                     /translation="MASLTAIQKDILQFLALFQHREGRSATGTEVQQHFGYTHHSTAR
                     QHLQALERKGFIQLARGGHGVPYHIRLLPPAFAMVDTLSLPVVGAIAAGSPREAIQQT
                     DRWVERLEDLLPVRPGDFLLTVNGESMIGDGILPGDLVLIRPQEVVEPGQIAAVMVRN
                     DEATLKRFYPQPPSHVRLVPSNPTMDDMVYPAEEVRVIGKYQGLIRTGHHGRQ"
     gene            15309..16163
                     /locus_tag="PLT86_00970"
                     /pseudo
     CDS             15309..16163
                     /locus_tag="PLT86_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            16188..17078
                     /locus_tag="PLT86_00975"
     CDS             16188..17078
                     /locus_tag="PLT86_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35221.1"
                     /translation="MLANTWASAIVSARRVYRVLDTAPDVRDTENAVWLPSSAEGRIV
                     FENVSFHYGGSHDGAVLEDVNLTIEPGQTVAFLGATGAGKTTLVHLIPRFYDVTSGRI
                     TIDGIDIRQIRQDSLLSVMAVVPQESILFSGTIRENIRYGKPEADDAETVLAAKAAQA
                     HDFILAFENGYDTHVEERGVNLSGGQKQRIAIARALLARPKILILDDATSSVDVETET
                     KIHDAIAAQHFARTTLIVAQRVSTVLKADKIVILDQGRISAQGTHRTLIESSSIYREI
                     CESQLTGSIPVDDSRGRAPA"
     gene            17075..18907
                     /locus_tag="PLT86_00980"
     CDS             17075..18907
                     /locus_tag="PLT86_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35222.1"
                     /translation="MMRASTREVRDVHAAMVPRGPMGMRGIIEKPRHARGAIVRLLSY
                     LKPFRGVLALVFLLIVAFTLCGLLGPYLMGVAIDRYIATKDVSGLAGVAALMLGVYVV
                     SNILQAATGWIMARVSQNALKQVRNDLFSHLQSLPLSFFDRNPAGALMSRLTNDVDAV
                     NQAVSQNITSLFGSVLSLFGILVAMFLLDRWLALATLVVVPIMFAFTRFVARYTRKGF
                     RRLQGALSELNSTMEESISGHKVVKAFRRSDAAMESFKKANDDVFRAGVYANTYALLL
                     MPLTGVLGNFFVIVLAGLGGWLALRDLVSVGVIATFIMYGQNFINPLRQLANMYNSIQ
                     AALAGAERVFEILDTPAEVDAGADAPPFEEVRGDVRFEHVRFGYQPGVPVIKDMTLAA
                     KRGTTIALVGPTGAGKTTVINLLTRFYEIDEGSISIDDIDIRRVRKADLRRKLGLVLQ
                     DTFLFSDTIMENIRYGRPDAKDEECMYAARTAEAEHFILQFPQGYHTDLTERAGNLSQ
                     GQRQLLAIARAILADPSILILDEATSSVDTRTEARIQKALLRLMAGRTSFVIAHRLST
                     IRDADQVLVVKDGEIVERGTHYELLERRGFYRHLYESQFKGETI"
     gene            complement(18989..21139)
                     /locus_tag="PLT86_00985"
     CDS             complement(18989..21139)
                     /locus_tag="PLT86_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOT35223.1"
                     /translation="MVRAVASAALRARAVIFLACVALAGVAVNPMVLRSTENPANVPT
                     QGNGNAALTAGAPASGGPFRLENKSYPPGRTLLVGKAGQVLEEPVRFLVTDTAGVPVA
                     GVSVVLEIVSRPKGAEDAEIASPVVSDSAGFVSANLSLGKAPGDYVISARVASMAGEM
                     PHVSAHALKGNWWLMVVMGIVGGLGMFLFGMELGAGGLQKVAGNKMRDILAKLTTNRV
                     MAVVAGAIITAVVQSSTATTVMVVGFVNVALMNLTQAIGVIMGANIGTTLTVQLIAFK
                     ISDYALLMMGIGVLLTLVTKRKVYIYTGEIFLGFGMIFYGMAVMSSSVAPLRILPAFT
                     ELMLYLAHAPFLGLLVATILSAIMHSGATIGLAVVLASEGLMTMDAAMPIILGANIGT
                     TVTTVIASLGTDTEGRRAALAHMLFNVLGTAIVFPILTYWNQWMRAVTLAMGSNSVAR
                     EVANGTMFFNVGATCAFLPFLPWFRKAVEWLVPSKETKEKAFGAKYLNNDLLETPDLA
                     LTAAYQEINRTADIVGEMHQYAMEAFGKDGEAVREKLATHCSEVETLHGQFQSYYIRL
                     AQKNLGLMQSREKQGHVTIMDDLRQISNFLGVDVVNTSARLAQVGAKFSDEGARELRQ
                     FHAFTVDLHRQTAQAIRERDRALATAVRGRKDEGEELERRLRDSHLARLDAGLQESAA
                     TSSAHMDMLTGIRQMGRHYFRICGALERFLTPPS"
     gene            complement(21259..23232)
                     /locus_tag="PLT86_00990"
     CDS             complement(21259..23232)
                     /locus_tag="PLT86_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT35224.1"
                     /translation="MGADAVFVNSLGMRFVHIPAGSFLMGNDRPLPDELVRLPNRRYG
                     DFDERPIRLVRLTKPFKMATTEVTNAQFEAFDPSHRQLRGKLGFSTGDNEAAVFVNWF
                     EACAFCEWLSRREGLPYRLPTEAEWEYACRAGTTTAYHTGDSLSAEFHKNVGVKWFPD
                     PSRGADEQEIPELTVGQTPPNPWGLYDMHGNVEEWCHDWYGPYDAHRPNDPVGALDGN
                     FRVTRGGSHSTELYYLRSSNRMGTVPEERSWIIGFRPVIGEMPSTNAIPGPIQLHESN
                     VKPEIPRDITCGPDPSKPFFCGPRTYVKVPPDSYGPMFSRHNHVPAIVACPNGDLLAM
                     WYTCMEEAGREVATLASRLRWGKDEWEPASLFWDAPDRNDHACALWRSKSGRLFHFSG
                     LSVAATWGPLAVVMRTSDNSGATWSKARLIIPEHGVRHMPIAGVIQLSDGTIVLPCDA
                     VTAGHGGSALWMSEDEGDTWYDAGGTIAGIHANVVELSDGRLMALGRGDDIDGRMPMS
                     LSEDKGKTWTYSASPFQPIHGGQRLVLFRLQEGPLLFVSFANDPLEITDATGTRRSIT
                     GLYCAVSFDEGATWANFRVMSDDGLPRPVEAFDGRPNILSASQSEVAGYMTVCQADNG
                     LIHLISSRNHYAFNLAWLKEPPPGEQHVRQVEP"
     gene            complement(23250..23978)
                     /locus_tag="PLT86_00995"
     CDS             complement(23250..23978)
                     /locus_tag="PLT86_00995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT35225.1"
                     /translation="MRYQIVFWDSGGTIFGPGRDGPLAEKPSPREVREGRVHRVSLAL
                     EMFGHRAPSDLGRVLQDTSSELQEKLGARYSLERLASGVYERLSLPVRPEETILVADA
                     IGGPRYRTWLWDGVADALKALAEAGVRMGVIADTDWTGRMMSRALAGVGLREYFGPVI
                     CSCDIGVCKPAPEIFAAARELIPDVPSGSPVLYVGDNLEKDVGGAIAFGWDAAYHVTT
                     KGGSSGRAVLDFVQYQDLVTLVTG"
     gene            complement(23978..24712)
                     /locus_tag="PLT86_01000"
     CDS             complement(23978..24712)
                     /locus_tag="PLT86_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT35226.1"
                     /translation="MRIVVHNVYWFQGCPSRWGVERVAAVPAVVDGLVELYASLSPDV
                     ICLQEVQDPSLVASAAERLGMASWIHAPGGVKTDYGGAILTRNPSKMRDLTSNGSQPP
                     HERVHIRAQVANGEESLEIACVHLPSNRFAGSPEGGSRARVAEAERILRCSPAPDVVV
                     GDMNSRKDSDPYLLMKSYGYVNAGETDVAEDSPERKGDHIWLLPAVARRLKKFFVITG
                     EPYVQNDTDGTVWRLSDHRPLVADLL"
     gene            complement(24795..25583)
                     /locus_tag="PLT86_01005"
     CDS             complement(24795..25583)
                     /locus_tag="PLT86_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35227.1"
                     /translation="MGVVRDRLWIWGHEAGSHNEGWGVPSPSRMTPAEAAWYMGIPNV
                     IFVTYQGKPEPPYDIYARAFVPLKNVVWSIVGEAGRTSDAAITGVRDVAAKFPNITGV
                     MMDDFFHDPKPDGSLAPISHDRLQSVRATLDECGRKLDMWVVVYTHQLNLPIAPFLDK
                     CDVVTLWTWRQEDLVNVERNLERIEALAPGKRVVLGCYMWDYGNKCEMSVENMKLQCS
                     AGLRWLTSGRIHGMIFLASCVCDIELPAVEWTRRWISEHGDTVL"
     gene            25829..26845
                     /locus_tag="PLT86_01010"
     CDS             25829..26845
                     /locus_tag="PLT86_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35228.1"
                     /translation="MARDEVDRVPVDVELTGEMRDKLLSHFGFREDEEIFRLFGRDFR
                     RVTARYTGPECYAKNGQPSDVFGVASGGPTYADTIGFRPFARAESVAEIESYSWPDPD
                     WWDYSDVPAQCERFREYAITGGEWSPFFCQACNMTGIGRFLELMLDAPEIAEAILSHI
                     VDFYVAGSRRYFEAARGGIDIFFVGDDYGGKNGMLMSPALWRTLIKPQLARLYALAGE
                     YGVHMMQHSCGSIRPVIGDMIELGLEVLDPVQIAAADMDPEGLKAEFGDRLTFHGGVN
                     TEDTLPFGTPADVFRETRYLMDTLGAGGGYVVCGSQYLQTDIPVANVIAMYKAAGSIS
                     QEII"
     gene            26853..27235
                     /gene="rnpB"
                     /locus_tag="PLT86_01015"
     ncRNA           26853..27235
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLT86_01015"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            27260..28615
                     /locus_tag="PLT86_01020"
     CDS             27260..28615
                     /locus_tag="PLT86_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35229.1"
                     /translation="MLLARSLPFVPERRWFRVAAWAAFALAVVARIGLRIHFTDFHSP
                     ELWEFGEIARNLVQTGTFTYRSNGVPSAFMPPGYPLYIAFLYQVFGVGEIAHVILAGT
                     LLVFELCLPFLVGYLAAEIRDRTTGFVAFLLALFWPQLLVMSGRLTNIPIYTSLLLLA
                     CVLVLANSIPLRRRAFLSGLALGMFGLFRFEACAFLVPFGFVLFRHKNGSGSRRAGVG
                     WRRRLGILSVFLGAFLIPLLPWIGRNYAVFGEVVLSTSGGYNLRRGHHARATGTAREH
                     YAAGNTLTESTAGGTPVPGADAMARVVERTPGDVLEVDRFFRDEALRFIAANPAKEAS
                     LAVRKLFFFLVADFTHPMSRVFPIWGTSFVALMVGLVHWFRSGIRDPRQQFLWLVFAT
                     QACLAVVFFVVPRYRMGVEFVPLVFFAAALDPLVQHWSADSTPQQKEAPVHRASPSEH
                     P"
     gene            complement(28628..29626)
                     /locus_tag="PLT86_01025"
     CDS             complement(28628..29626)
                     /locus_tag="PLT86_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35230.1"
                     /translation="MAARVRVAVLGAGAIAQDHLRALRFSSRAEIVGIADVNLSLAQK
                     AAAEFGGTAFADYHDMLDRLKPSAVVVCTPPKFHRQHVEDCARAGSDVLCEKPLALTV
                     PECDFMISAVKKAGRQLMAGQVLRYYAGPKTLIEIARSGKIGTVVTCWSTRVGYYDPA
                     HGPSWRYDPKIGGGTAIEWEVHEIDLLRMLAGEAKTVSGRVLYTRPDSPEFDDHVHAV
                     MTMGEGAIGRIDASNSSYYSECTRGVVGTTGAAWCGWGDVSLRTAGSNDVQIVKPVLP
                     EVPENVNVGMFQQDDAFLEALQADRPVPIPGEEGRADVAVAAAILLAGKTGETVRL"
     gene            complement(29661..30689)
                     /locus_tag="PLT86_01030"
     CDS             complement(29661..30689)
                     /locus_tag="PLT86_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35231.1"
                     /translation="MYRVGIIGAGWVAGEHMRAYLNNPETEIAAIAVRRPETAQAYQK
                     SLGFSCAIYTDVAKMLHEADLDIVSICTPPSMHPEHAVAAAQAGCHLVLEKPVALNVE
                     GFRQIRAAVEAAKVKTVISFVLRWNPLFETIKALLGAEAIGRLFYGEVDYNHGIGPWY
                     GQYRWNRLKAEGGSSLLSAGLHALDALRWFVQKEAVEVFAYSTNSMNLELYPGGYEYD
                     PTMVTVLKFKDGVIGKVASNIECVQPYTFPITLQGTSGTIRNNQLYSKKLMPGQTGFA
                     TIPTILPDSGDVTHHPFQHEIDHFVQCIQLNVESHANLVDAAKTHEICYAAELSAAEG
                     HPVRLPIL"
     gene            complement(30893..31192)
                     /locus_tag="PLT86_01035"
     CDS             complement(30893..31192)
                     /locus_tag="PLT86_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOT35232.1"
                     /translation="MLTAIMLINAELKSVAAVTRSLAEIPEVRQLFTTTGQFDVVAVL
                     VVKDSEALAEAVTERIANVPGIVRTSTLLAIRCHAQSLLQSTFSVGFEPAEPSQS"
     gene            complement(31300..32241)
                     /locus_tag="PLT86_01040"
     CDS             complement(31300..32241)
                     /locus_tag="PLT86_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT35233.1"
                     /translation="MARPLGMATRQAYGKALVEIGKLHPNVVVLDADLSKSTMTKDFA
                     KAYPDRFFNVGIAEANLVGVASGLATTGKIPFASSFACFLMCKGFDQLRLGVAYAENN
                     VKIVVSHGGISIGEDGVSQMSVEDVALACELPKMVVMVPSDEHCTYQAIRAAVEHKGP
                     VFVRTGRAKAPLVYDAPPADFAIGKAITVREGKDVTVIANGMLVGPALDAAESLAEQG
                     VSVRVLDMHTVKPLDEEAVLKAAKETKGIVVAEEHLVHGGLGSAVAQVTARMHPARIE
                     FVAIQDTYAESATGEELMAKYGLSEPDVIAAIRRILA"
     gene            complement(32268..33098)
                     /locus_tag="PLT86_01045"
     CDS             complement(32268..33098)
                     /locus_tag="PLT86_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT35234.1"
                     /translation="MAQYGPPQTVSQLEKTAKELRRDVIRMLYEAKSGHPGGSLSAAD
                     IVAALYFRVMRHDPQNPSWEKRDRFIMSKGHAVPIVYAAMAGTGYFPKEELLTLRKLN
                     SRLQGHPSAKDLPGIEASTGSLGQGLSIGLGMALGLRMDGSDSRVFVILGDGEIEEGQ
                     VWEAAMAAAHFKTENLIAILDRNRYQLDGSTDEIMNVCPVVDKWKAFGWRVLDIDGHS
                     MHQVVHALEAAKEQTGRPTVIVAKTTKGKGVSFMENDNEYHGKAPSKDEMERALAELS
                     "
     gene            33756..35894
                     /locus_tag="PLT86_01050"
     CDS             33756..35894
                     /locus_tag="PLT86_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT35235.1"
                     /translation="MTDPTGLDSELELHRWMGARIRQIRVTKNMTQDELAQRMGTQRP
                     AVSNWENGINMPSLFTLRKMAATFGVSLRQLIEPDSEDVADVPMIPLDTAVDRPGLER
                     WIRLYERLTELGDSGQRLLQTTLRIFGRLGALGDSGSGQVVYSSVLHAIQEALNFGLT
                     VLSKDDFRRTTLFLRETGLAADTEADQDRYAHLHQVFVPPAGYVDLLARMTGEQRVVV
                     ITGETHQGKTFVATKLLADIAEHGYRPIAVMGCFSGLAGDTARADTRLRDCLRPGHAV
                     LLDDPFSPGAGALALREFTTNPNLALSLVAEADCRLIITATADALEQRPDAARQLAPY
                     EWSLADKYEPTSFVRMVINYSEIYHPGAREPIVASHEGLSSPHAVERSFAVATDNKQT
                     IRRRSAGIRREGVEAITVADYDTLDNAELAFLLLLRHVPLPAHELYSIFTRLEIQGIS
                     GVVPTYDVLLDDLRRWLLVSKQDGATNIGFYHPSYASALDSILWERERVQRLAGRVLR
                     ELVTAGDPYLDRAAMSLLSVISVAGGAALGSLLREAGLADGVLLKNADPAIASLIHGV
                     MGDQGEQLAADLKGLCEVSFTKRYLSSFSLISQQARGANAASGSPQQVWNDMVNGYLS
                     DNWQVNRDLLTVLASAAVSGNARMRALAGTLAIVRGSQHDAHFSAKLIEPLQDDRQKG
                     VTRCTKRALLDYFDLVPASARDELLAATGL"
     gene            35920..36852
                     /locus_tag="PLT86_01055"
     CDS             35920..36852
                     /locus_tag="PLT86_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT35236.1"
                     /translation="MEYRILGRTGLRVSEIGLGALEIGRDWGIPVGTDFGRPDEKGAE
                     RLLNAALDAGITLIDTAPAYQLSEERIGKAISYRRHEFVLATKVGERLNDGDAKSHYD
                     YSYEATLAFVEQSLRRLQTDVIDVLQIHSASIEVIRAGETLRAMKKMQEQGKVRFVGM
                     TGNVDAAIEAVKTDDYDTVQVSYNVAYREAEHELFPLCLDNNIGVIIKDGLARGSLTP
                     KVSALPEKAKKQKLVADRLISECVAKSLHPNAYPKSLSDFALRFVLHQKAVTSVIAGT
                     RKVEHLLANVGASDGRRLTDEMVRKAIEIVDSVE"
     gene            36880..37653
                     /locus_tag="PLT86_01060"
     CDS             36880..37653
                     /locus_tag="PLT86_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT35237.1"
                     /translation="MIALSGCAEALFTTLPFNERIAAIAGLGLPAFEFWGFANKDIPG
                     IRAAMEKTGLAVSSISCDTGGPLVDPSKRSAFLPACKKSIEVAHLLGCKTLIVTVGQE
                     IPGVSRKEQHRSIVDGLKTVVSLVEDEGVTLVIEPLNVLVNHKGYFLATTSEGIDLVD
                     EVASPNVKLLYDIYHQQITEGNLIDTIKANVAKIGHFHMADVPGRHEPGTGEINYEQV
                     FRAIAETSYSGYIGLELWPTKPEKEALASTIAWAKRYFG"
     gene            complement(37717..37803)
                     /locus_tag="PLT86_01065"
     tRNA            complement(37717..37803)
                     /locus_tag="PLT86_01065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37767..37769),aa:Leu,seq:cag)
     gene            complement(37888..>38917)
                     /gene="dgoD"
                     /locus_tag="PLT86_01070"
     CDS             complement(37888..>38917)
                     /gene="dgoD"
                     /locus_tag="PLT86_01070"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HOT35238.1"
                     /translation="SLYGKEHTMRSAIEELRRDIVGKDPRRVEYLWQEMYRHAFWRGG
                     PVLCSAISGVEMALWDIKGKLLGVPVYELLGGHVRDEVRFYCNLGDGTPESLIERGKK
                     WIAKGIDAFKIYPFPAVNYVDSMRPIREAEEKIASFREFAGNEVDIMIDFHGRLSPSM
                     AILAEEVMRPYEPLFIEEPCLPENVEAMAKIARHAKTPIATGERLYTRWGLREIIERQ
                     IASVVQPDLCHCGGIMEAKKIAAMAETYYMATAPHNPYGPVATAACVQVDFCIPNFLI
                     QEHIFDDMPWRDLVYTPVIELENGRLKRPTLPGLGIDLNLEEIAKHPYEERDQVKWYH
                     PDGAIADM"
BASE COUNT         7915 a        11798 c        11103 g         8101 t
ORIGIN      
        1 aaggcgtgcc cgttcccgag aacactgcgc acctggttgg agtccggcga aatgatgatc
       61 gtgatctcgg taatccccgc gcggcgaaga ccgtcgatgt tgtgcacgat gagcggtttg
      121 ccggcggccg gcaggagcgg ttttggcacg cgttccgaga gcctcccgaa ccgcgtgcca
      181 cgcccgcccg ccagaatcag tcccagaagt tgtttctgca cttctcgcat ccatcacgtc
      241 ctgtcacgtg ccgcggatca tccctcgaat ggcgcgaaga ccatctgccc tgccgggaca
      301 atcacgttct tcgatcttcc gccggggagt cggacggttt tcgtcacgtc cgaattcacc
      361 tgactgaaga acatcacgcc tcgtacgcct gctctttcca taagagcgcc gtacacttcg
      421 tcccgtacgc catcgaagat cgcgtaatcc tcaacgccca catccagttt cgacgcgttc
      481 gccagcgttt ttgtaagcat ctcccagaac gcagggtgag ttgccgggtg gtccttgtgg
      541 tcgtctgccc agcgatcatc cccaaagtac cctttgcgat ttgcgcacac gggaagcatg
      601 acgacgcacc ccttgcccgt ccggcacatc cggccgccca gttccttctc aagtggactg
      661 accgtcggct cgaaagccat aaggcgcaat cccagagaca ggtctccttc cacggtggag
      721 ggaacaccga ggttgtgcgt aatcagaaca cccccgtttt ccacccaccg gtacagcctc
      781 tcaagttccg gtccctcgta cgtcgagccc tgaagaatca ccagaaaatc gtaatccttc
      841 ataatcccgt cttttatcag gccatcgtca atcaggtcgt agtcgatgat gtccctcagt
      901 tcttccgcct gcggccagaa cctgtcccgc atcccctgct ccgaaaaacc gtgatcgtcg
      961 gcaagaacga aatgtgtcca cgggtagaac acggcaaccc tgttcacacg acggtactcc
     1021 ccgttgagaa acggaagacc ccgttggagc gcaaggcgtc ccgaaggtga atggaggtgc
     1081 ccctcatacg accagaagtt cgtcccgtgt tgcgttacca ggttgaaaac ccttccgaga
     1141 acgccgttcc cggccatgtc tccgccccac ggttcgtttc cgaacggcag accatagaaa
     1201 cggcatccca ccccttgcca cgccgtgtaa acgtagttga aggcgtagtc acatccctcg
     1261 tttgttgcac gatgtcccat gcctgtgtgt ttcaacgcgc gtgtctggtc agtccagtgc
     1321 gcagctctcg gtggatcacc tgctccaccc gccgcattgt agaaaacggt gttgggcatc
     1381 gcctcatggc agatctcccc ccagcgctgc atccaccacg tcatctgacc gcaataccag
     1441 ttgatttgat cgatccacgc cctgtcggac ggggcatctt tgcgcacaac ggggcggatt
     1501 tcgtcgaacg atccgaacga tgaagcccac gcttcgttca accgtccgat gctgccgtat
     1561 ttctccctca aggtcctgcg gaagtcagca acggcaacat cttcgccgca ccagtacccc
     1621 acgtgcgtat ggtacatgtc gcccgtgaag acaccgttcg tggtcgactc accgaaatcg
     1681 ccggaaatgc ccaccatgat agattcaatg agttcctccg ggtaatgctc ggcgaatttc
     1741 cgcatgaacc gttccacgta atcgagctga accgggctcc agatagactg cacccagctt
     1801 tcctgtccgt gttccaggca tttattgcgg taattgttcg acgtcgcgag ccaccacttc
     1861 ggcatggcgt accatggccc gatcatgata aatgcaaccc attttagtcc gaatttcttc
     1921 gcttcggcca ccacccagtc gtaaaaagtc caatcccaga cgccttcttc gcgctcgata
     1981 cttgaccagc ggacatagga ctgaagcgca gttacaccca tgcctttgta caggggaagc
     2041 cacggcggaa cgctcagcac gcccgaatcg tcccgttcct cgaaaattgc tcctatgttt
     2101 ccgatcgaaa ccggaattga cgccctgggg taggactggc tcaagtgttc ttctgcacgg
     2161 cgtgcgtgag ccatctggtc ggtatctgag aagtcctcag gacgttcttc ggaaacccgt
     2221 accgtcgaaa gcacaagcgg acccgacccg attatcttga ggtcagcgtc gtgaaaaccc
     2281 gagtggtcaa aaactggctc cacaagccgt atcgtcgccc gctgccagga tcctgtatcg
     2341 agagtattga cccaggttga gtcccatggt ttggacgcac cgggcgggca cacgtggtgc
     2401 acaaaaccct caagtccatt ccggtggtac tgcaccgcga tgaacccgag ttcgctttcg
     2461 aaaaagtcaa tcgtgacata cacctcgcga agggaatggg gcggcggact ttcgatctga
     2521 agccagaaga cgccgtcctg aacatcaaca ctctcactcg tgccacgcca gtaacctccc
     2581 accgaccctt ttttgccaga aggaaccgcg cgggcgcacg cccttccgga tacatgcgcc
     2641 ggcacaacgg tacggttgtc tgttatgggc ttcgtttttc tgccaacgcc ctggcgaagg
     2701 cttattccgt ctgctgtcgt cgcgtcggag aatgtgatgg acgcactcca ctggttcttc
     2761 tctgccatga tagttccttc atcaagtgaa actcagaggc gaacggcgcc ggggtgatgc
     2821 agggaacctg cgcgccggta ccgtcaagtg gtggaagcga agacaccctt gatttgagtt
     2881 cgatactgcg ccgcatgccg gcgcaggtga cccacgttcc agagaaggta acaagacacc
     2941 tttgcggggg taatggcccc tttggcctga caagtgtggc gacggattcc gcgggggggt
     3001 gagtcgcaag agaaccctcg cggaattgcg gttggcgcgg gtaagttcag cgtgcaggga
     3061 gtggagttag aaattaaaag gcgccggcga gacatcacgc tcgccagcac ctttttttat
     3121 cgggttcttt gacttcttca aacgctcttt ttgctgaagt accagtccac gacttccatg
     3181 tccgtcgctt tcatccgctc ttcagccgcc tgcgtggtgg ccggcggcgg cacaatgact
     3241 ttgtctcccg gcttccaccc ttcgggcgtc gcaacctttt tggctgcgtt gatctgcagc
     3301 gcatcaatca ggcggatgat ttccggtatg ctcctaccgg tagtgagcgg gtagtagatg
     3361 attgctttca ccaactgatc gggatccatt acaaacactg cacggaccgc gctcgttgtg
     3421 gaggccttcg gatgtatcag gccgaatgcg ttggcaacct tcatgtcgag gtcggcgatg
     3481 accgggaact tcactttgac accgaagagc gattcgatgc tccgaaccca ggcgatatgc
     3541 gaatggacag agtcgatgct gaggccgatg agctgaacgc cacgcttgtc gaactcttcc
     3601 tgatggttgg caaacgccat gaactcggtt gtgcacacag gagtaaaatc agcggggtgg
     3661 ctgaaaagta ccacccactt gcccttgaag tcgctcaact tcagcactcc gtgggtagtc
     3721 accgcctcga attccggggc cggttcgttg attctcacca gacccaccgg ctgatcctcg
     3781 cagcaatcct tttcgttaca catcttcgct tctccttggc atggaccgct ctcaagcccc
     3841 cctcggagtt gagttgcgat ctcgtctgtc catagataag gtctcacaaa ttctgttagc
     3901 tggcaagctc cgagaacaca ccggacacgc agcctgtgag ttcggattga cagcaggagc
     3961 gctaacgtat atattacact tggattacag gatgttgccg tggaattggg cccggaacaa
     4021 cggattatcg gcccggtaat ggccgaaatg acgtcgttct ttcgccgcgg catatggaac
     4081 cgccacggtg ggttgggtgt aattgattga aggaatcggg gggcggaggt tgaggaagtg
     4141 gatgacataa ggttcaatgc tcgcgagcgt cttggcgaaa aacgccgcgc gttcgtctgc
     4201 cgattgacag acgggcgtgt cgccatcatc tatgatggcg cgggagagga cgaaccccct
     4261 gccacggtaa acgacctcga tcaggccaaa cggcaggtgg cgcttctggt tcccgatatt
     4321 cgcgtgccgt tgtatgaaga gcacgaactg aaactcgtga agcagaaact cgcgggcata
     4381 tcctccctga gtccggtgtg aggcaactca caccggccgc ctttcagaat tgcggaatca
     4441 atcccatttc tcgcggcgtt cgtctacatc ccatattgtt gttgcctgcg tcgttccccc
     4501 tatcacctca agcttgccgt cgccgtccac gtccccgagc tcaatgattt ccccgaacgg
     4561 ggtggtgttc tcccattcca gattgcggaa tgccgcatcc agaacgtgcc cggaactcag
     4621 cacgatttcc tgttgcccgt cgccatcaac gtctcctacc gcgatatctg ttgcacggta
     4681 caggttgtcg gagcgccatt tctcgacaaa atgaaccgcg tcgaaaatga acaacctgtt
     4741 ctcgctcaga aacaggatct ccattcttcg gtcgccgtcc acgtcggcaa ccgtcattgc
     4801 ttcgatggac cggaactgcg tttcctgcgt tcgggaaagc aatgcgaacg tgttcacgtc
     4861 atagacgtac aggcctcccc gggtatttgt ggcgacgatc tgaaaggttc catcgttgcc
     4921 gagatcagcg accaaaacct tctggattcg ggacagaaga ggcggactca cccagatctc
     4981 ctggtaccgg ctgccggtga gctccagaag atggagatat ccgcgcgacg ttccgtacac
     5041 gatgcggcct ctcggatccc cggggactga agcgaacgtg tcgaatcgcg atgcaagccg
     5101 cctaacgggg tcgtgcacaa gaggcacgct ttcaggctgc tccggtggtc gaatccgcgg
     5161 ggctgcagca accagaacga cgatcgtccc cgcaaggagc atccatctga acgtattcat
     5221 ggttcctccc ccttaccgcg atttgtcggc ggctttgcga acgaattcgt atacctccag
     5281 aaccgggacg cttctggcac gcgcgatcgc ggcgcagtcg tcatattcag gcgtgatccg
     5341 cgtccgtcca tcccattccg cacatttcac gcggacaggt ccccagggtg tttcgacggt
     5401 ctcacttcgg cgggggagga cgcgccggac aacgtcggat atcctcacgc caagcgttga
     5461 tgtttccccg aacaggaccg atatcactct ttcccgctgc tccgggtgca caaggacgtg
     5521 gacgacgatc cccggccggt tctttttcat gatgaccggc gacaggaaca cgtcctttgc
     5581 accggcttca aaaaggcgtt caagaacgta cccgtacact tccggcgtca tgtcatcaat
     5641 tgtggtctcg acgacaagcg cgcgctcttc cccgatagaa cctgttgtcc ggcctatttc
     5701 aacgcgcagg agattcgcga tctcctgcgt atcacggctc ccggccccgt agccgatcgt
     5761 ttccggcaca aacacaggag ggcacgcgaa gcttttggca aacgtcgtaa tgatcgccgc
     5821 accggtagga gtgacgagct catacggtaa cgaggttcgg tcgaccggca caccgcggag
     5881 caactccaac gttcccggcg cgggaaccgg catgacacca tgcgcggccc gtatcgtccc
     5941 gatgccggtg cggattgccg acgcgtgaac ctcctctatc cccatcgttt cgattccgag
     6001 gcaggttcct accacatcaa ctatcgcatc aaccgctccg acctcgtgga aatgaacctc
     6061 ttccgggtca gttccgtgaa cgctggcctc cgcctctgca agccgtttga aaacgctgat
     6121 cgcgtttttt tccacaccct gagagagtct ggcggtctga atgagctcta ccacgtctga
     6181 aagacggcgg tggacatgcg ccgcaagggg aaccggatgg tcccgttctt ccgaggatgg
     6241 cccaccttcg tccagattgt gatgtccccg gccagctccg tgcgtgtggt gatcatcggg
     6301 cccatggtca tgcggatgct cgtggcgagt gtgttgaagg gaatcggcgg aggtggcgtg
     6361 ggtgtggtag ggcacctgcc cgtggataac aacatcgaac ctggttgccg cgatatgatg
     6421 tcgccgggtt gttgatttcc ggagctcgta cccttcgacg cggagctttc gaagttcggc
     6481 ctgcaggtat tcgaaatcga ggccggcgtc aagaagcgcg gctaccgtca tatcaccggc
     6541 aattccggcg aagcaatcga aaaacgctat cctcatcggg cgccctgcct cgttctccgc
     6601 gtctaaccgg gacactctat agtttcggga atcactgcgc gcgcgttgaa cagcacttcc
     6661 cacgtcctta ttacgcgcgc cagccgcttt ttcccccagg gtccagccgt aatgacgaca
     6721 gaagaaatct ccctgcccga acgggcttcc gtaagcggca cccagtccgt gccctcggac
     6781 gctcgcggaa caccggttgc cggatcgaaa gaaggcagct ccgaactggc acgtacttct
     6841 ggtgttacta ccgtcacggc ttgcttcgtg caaactttca gtcgtcctgc aagccacttg
     6901 ccagcagggg gaagcaccct gctgtttgcc tgcccttcat gggcgttgga cggatgccgg
     6961 taactccgag ggtcgtcacc tgataccatc agcgtctcct gctcatacgg ttgaacgttg
     7021 ttccgcttcc ttgagacccg gtgttaccca ttcctcctgg aacacgacgt ccggaagccc
     7081 gtcgtcccgg ttcccatcgg cgccgaacgg gtggcccatc ccgcggaccg taacagttga
     7141 ttcccaccag atgggatcgg gaacatcctt cacctcggcg ccgaaatgtt ctacgcgaac
     7201 ggtcactgag gcggccaccg cgcccttttt tctcagttcc tcctgaacca gtggaaccaa
     7261 cacatctcgc gccacggcaa tcgcacgttc ccggtccgca gttgaaatgc gtttttccgg
     7321 cgtataacag acgatgctcc cattttcctc cgggcgcaaa agcgccgtgg ccgtcgcgac
     7381 gaccgcaccg gtggcagctc ccacggcgtt cgcaaccgga gcaccttccg gaagaacaaa
     7441 ggttccgcca agccggtccg acactgagga aagaaacaga tgcgccggcg cccccacacc
     7501 gataatgggc gggcgcaggt tgagtgaagc gccaagcccg tttcggggta cctcacgaag
     7561 aacgatcctg gtgaggtgcg attccagcgt gtgcgcaccc cggtggttgc cctcgtccgg
     7621 cacaccgaaa tatgcatgca tgacttggcg tgtgagcatc tgcgtggcga tttccccgac
     7681 gcgggcgaca acctcctccg cactgacacc gagccgtgcc gcagcaagct cggttgcgat
     7741 gcgagccgat tccttgtccc agggttgaaa ctgacccgtg acgtgccaga tgtctgttgg
     7801 cgtaaggccc acccgttgga tcagcctcca tcgttccagg cgttccgttc ggagaagcga
     7861 ctggtgcggg tatccgaccg ctttcgccag accccatcgc gttttcggac cgttttcgag
     7921 tgcgtccaga atcgtctttt cctgatcctc aagagcggac cgcgcttccg attggagtcg
     7981 caggaacgcg tccgtcgggt tggtcaatcg ccagtctcca cccggcgaac cgtctgagtt
     8041 cactcgttca agcaattctc tgtattcttc acgaagagcc gcgcacttgt gcccggcata
     8101 ggaaacaggt atcacccgct gaggcccgac ggaaagtttc ccgtgctgaa ctgcgaccga
     8161 gctatccccg cccaggccgc gagtgcagac atccgccgaa cggactccgg cccgccattc
     8221 gcccacggag acaccacccg gggccatgga cggtcgtcca ccccgcaata cggcgatatc
     8281 ggttgttgtg ccaccgacat cgacaacgat tccctccgca acaccggaga gccgtgcggc
     8341 acccagggcg ctgcacgcag ggccggacag aatcgtttcc accggcctca gtcgggccac
     8401 cctcgtggcc atcaggcttc catcgcccgt taccatgaaa cacgggatgg cggagagccc
     8461 cacctcagac agcacaccct ccaccgcgtg caggaactgc tggacgatgg ggatcaggcc
     8521 ggcgttcaac gcggccgttt cagcacggac aagggcattc agtacgcccg caacttcgtg
     8581 tccgcacaca atcgggagtt ccgtcagttc atgcgcgatc tcggccgcgc gcaattcgtg
     8641 ctcgggattt ctcacactga gaacgctcga aatcgcaatg ccggataccc cttcctccag
     8701 aagctcggtc agcgcaaccc gtaacccggt ttcgtcaagt ggctcgcttt ccaccccacg
     8761 aatcgtgtgg cgccccgcaa ccactctttt gggctgccac cgaacttcgc gcgcctcgaa
     8821 tccatcatat cctattaata ccagccccgc aggggcatgc cgtccctcga caatcgcgtt
     8881 cgtagccagc gtggtcgaaa gcgcggcgta cgttgccctg gaccggatcg cggcaggcag
     8941 accggcaatg gctcccgcca cgccttcgat gtaggagggg ggcgttgtcg gcgccttcgc
     9001 ggaagccaga atgcggtctt ccgaaaggtc gtaaagcact gcatccgtgt atgttccacc
     9061 cgcgtccaga ccaagtgcga tcggcatata ggtttccatc ctgcgttatg tgattgttgt
     9121 caacgccatt taatcggttc cgagtaacaa aggtacccaa tccccgagcc tcaccacaat
     9181 gagaaggtgc ctcccagcgc acggggttgc atcggaagca aacctgatgc actctctgaa
     9241 ccatattctg gtttcgacgc aacatttccc gtagggggct tgccatggtt gcaaaagcgc
     9301 aactcgaccc gtcgccgttc ggaatgggca tctacccgtg tcacagaacg tctggcgaaa
     9361 cgacactgag ggcgcttggg ctcgcgcggg acgcggggat caagtggacg cgagatgaac
     9421 tcggctgggg aggaatccaa cccgaacgcg gagtctggaa gtgggaagcc tttgaccgtg
     9481 ccattcgttt gacacgggaa cacggcattc atacgctggg cctgctctgc tattcagcgc
     9541 cgtgggcggc aacccacaga acggctgagg gaaaaccatc cgtcgtgagc ttaccagatc
     9601 tgagcgcctg gaaggaatac gtgttcagaa cagtggagcg gtaccgagag acaatccatg
     9661 tgtggcagat gtggaatgaa cccaacattt ccggattctg gcagccaaaa ccgaatccac
     9721 gggaatacgc gaagctcctc gtggccggcg ccgaggctgc ccgtgcggcc gacccgacgt
     9781 gttggatagt cgggtgcaac acatccatgg tggacctggc ttttgaccgc gtggtgtttg
     9841 aagagggtgg gtgggatacc accgacatta ttggagtaca cccgtaccgt tatcctcacg
     9901 cccctgaaca caccgacatg ctgggcgagc tgcgaaacct tgccgcgctg gcgtacgagt
     9961 tcggggcggt caagcccatc tggatcagtg aaatcggata cgcaacccat ctgggacccg
    10021 gtggaagcag tgagtggtgg tccgcggcga tgcttatgcg agtctacctc acgtgttggg
    10081 ccagcgggct cgtgcagaag gttttctggt acgattaccg cgatgacgga accgacccga
    10141 catacaacga gagcaatttt ggtattctgc accacgactg gtcacccaag atgccgtacc
    10201 acgggttcaa ggcgatggca catgcgctgg aagggttttc gcctgaaggg cgagttgatc
    10261 ttggcgaagg gccgtacgtg ctgaaattcg ttcggggtga cgaggtccgc tacgcggcgt
    10321 ggaccgtggg gaagaacgcg cgagtgccgg ttccggcacc ggcggatcag gtgaccgtta
    10381 tccgggcgtg ggatgcgccg cttcaggcag aagcacgaaa cggctgggtg tcagtcgccc
    10441 tggacgcggc tccagtattt cttgtggaca gccggcggtg gactacgtca taactggcat
    10501 gccactgacc gacggatcag gaagctcgac cagtctatcc cgctggtcaa gcgccagcca
    10561 gaggtgtcgg ggctcgcgcg ccgtgaactc gcttctgccg gcccgcacgg tgtaaccgga
    10621 tttcgaatcc cacaccacgg tgacgtcaac atcgccacgc cacgtcatgg gaccgaattc
    10681 caggtgttcc cattccgtcg ggagcgcaaa tggccagcac tcgatgatgt cgtcatatcg
    10741 ggggacgaac ccgaccacga agcgcacgaa caggtcgagc gtaatggcgg actggctgca
    10801 gaaggtgtgc gggtccatgt tcggcgcgcc cgtcatggaa ttgtaggagt acgaggacgt
    10861 cggcgcgttt tcgcccagca tgcgcagcgc cttccagata attctcctcg cctcctcgag
    10921 gaagccatac ctcaccagag cgacgacagt aagccagacc ccgctggtga tcagcacatc
    10981 acccccgtac gtgatgtggc ccggatccag atccggtgtg tctgcggcaa ccgacggaac
    11041 cggatacggc gtgccaaatg cggcaggatc ctgcaggaga cggaccattc gttccgcacg
    11101 gtccgccggc gcgactccgg acatcagcgc cgagaacgcc gccaccgaac acacgccggt
    11161 gagctcgtgc gtcacggcat cgaggtcccc gtaccagccc cgcgtttcat cccacatgag
    11221 atcgttgatt gccctcgccg tcttgttgag acgctggcga atccgttttg cgaggttcct
    11281 gtcgccaact atccccgccg cttcaattat gtcttcccga aggaggcaca gttgcgcgtt
    11341 tatgtcgatc agttccagcg gcctgtcaaa ccggatcaga gattcggtgc ctttcttgta
    11401 cccgttcacg gcagtgcgcc agcgcaggga gtagtcgtat tcatcaacca tcccctcgta
    11461 ttccacaagg ccgtctccgt ccgcgtcatg cgatgtcagg cgggcttcgt aggcaaccat
    11521 ttcgggaagc tggcgacgga ggtaatcctc atcgcccgtt cggcggaaca cttcccagat
    11581 tccgtgcacg ccgagaggcg ttgtgtgggg cataatgaac gcccagtcaa actgacgcgc
    11641 agtcgccggg aggtttttca cgccaccgat ggcgtcctgc aatgcgggca ggtattcgtt
    11701 gacatccctg aactgccgcg gtttgcgaag cctgtgaagt gaagcgccta tgttcagcgc
    11761 cccgccgttt tctctcagca attcgatacc cgactcggcc cacgccggat cattcagcca
    11821 gcgctcggcg atgctgttca tgggagtatt ccatgaccac tgccacacgg ctccggtttt
    11881 ccagggacat gtatacgggt acacgaaggg ttcgtagggg atatcgtaaa ggttgatctt
    11941 gtgcaatgcc gtctggtagt agtaaagccg cctcacccac tcttccgcac ctttgagccc
    12001 gggaaccatg cgcttcatgt accactgcca tgcgtcacgc gccttcgtca ggaacaattc
    12061 cgcgatgtca gccgtcggcc gattcgagca ctgcccgatc atcgcctcga gccggcgttc
    12121 gactgctgcc tggtcgtaac cgagcagcag cacaagcacg cccccctgtg gcggtatgga
    12181 agcttcccaa ccgaacttcc ccgaggctgc tttcccttgc cagttggtta cctctgaacc
    12241 gaaccagacc catccgtggg caatgccgtc gtcgtgcatg cacacccggg ccccgtcgtg
    12301 catgcgccag gaacccgaga gtgttctttc caccgaggaa gaccacgccg cggacaaggg
    12361 taattcggag gttccctcaa ccggcctcag ccagaccacg atggcgtcct caacgatcgt
    12421 caccgtttcc tggagcgtga cttccgggga gacaaacgtt cgcgttatct ccgcaggaga
    12481 ccactggaga ttctgcgtat cccaggtgac aggttttcca cctgccatca atgcgccccg
    12541 ggggccgcga agaacgtgga ccgagctctg tgcgattctc ccgctgtcaa agcgttcgac
    12601 ctcgaggtgg ggagcccgcg gaaccgggta gaaccacact ttctcgaggc ctatagaatc
    12661 aggagacgtc cagaggctca cctgattgtc ggtcagaaaa cgaatgtccg ggaggcgaag
    12721 gagatcctcc cacggcgcgt cggaaacggg aaggtccttg gcccattccc atttctgccg
    12781 tgggtataac gcggggcgac gcggcatggt tactccactc gtggaaagcc cgtgaggtga
    12841 tagctgcaga cacgcaaaca ctgagggagt cacaagaata ttaactttaa aaaattcaac
    12901 aatcgctggc cgaagtcaat gggggcggga gaaaaacggc ggtgagaggg ttatctgtcg
    12961 cggtccctgg ggaaatcaaa gaagggaccg ggagccgttt gaggaaacgg ctacgctgat
    13021 gcctcgagtt tcactcgtat ggacggcacc gacagcggct tgttcgctcc ttggtgagac
    13081 ggcgcaatag aagtggaaca gccgattcct gtgccagaac attgacggct tgtgggcgta
    13141 ccgcgaatcg accgcccccg gcgggcccac atcaacgaga acagaaccgg atttctccca
    13201 gtggagcaga tcgcgggaga acgcgacacc atctcgcgcg tggccatcgc gaccgagccc
    13261 gaaatagaac atcgcccatg cgtcgccaac gcgcagaaca caggggtcgc tggcgaatcg
    13321 ctcatcaaac gcgccttccg ctcctgtgcg cagaatcggg ttcccgccgt atcgttccca
    13381 ccgcatgagg tcgcgtgaga ttgctacgcc ggtctgttcg atccagggta ccccgacagt
    13441 cttcgcattg taaaaaagat agtacctcct ctggaattcg atgaggcagg atttataaag
    13501 tcccccacgt tcccattcct ccccttccgc gggaatcaga cagggctcac cgatttccca
    13561 gtcgcgcaga ttcgtgctgg agcagatgcc gatcaccgcg ggtccacttt catacccctg
    13621 cgcggggtag gcgtgataag tcccgacgta ccgaccatca atctggcgga ggcatccagt
    13681 tccaaaaaga tcgttgtccc gcaagatcca cgtcagagca gcgttgtgct gggtcaccga
    13741 tccggcgatt ccgcggtcga ggatcattcc ctccctgtgc cagacgagaa gatcgtcaga
    13801 ccacgcaagg ccagttcgat acccaaggcc gtcaaacccg atatacgtca tgaggaaacg
    13861 atcctcatga cggaacacga acgggcaatc aacgccgtgg ctgtcgaaat cgcccgggcg
    13921 gaaagagggc gacaggatga gcgatccgtg tttgaagggg gtttgaacgg ttttggcaag
    13981 cgattgtgcg tccatcggtc cggtgtcctc gccgtcgatt tacacggcgg aaacgcgcga
    14041 agtgtatcgg ttgagaatca gcgccccggc aggagattcg gtgtcaagcg gaaagaggcc
    14101 ggcagcccat cgcacacgga agctggttcc cgagagggcg aaacgaatcg ccgcgggcgc
    14161 tgacaccagg atgatgcggc gcctgcgaat acgggcttcg gtcacggcca tcttgatagc
    14221 ggataagagc ctggtgtcta tcttcgcaac gccttcgagg ctcaacgtga tttccttgcg
    14281 agcgctgcgt tgcaatgccg cgatgacatc ccgacggagc tgcaaaggat cgaccattgc
    14341 gctgtcgtcg acacgcacct gcacttcagt acgctcgtct gtcgaatgga gtgtaagcgg
    14401 cattgtaagg cccctctgat gatcgcggct actggcggcc atggtggccg gtgcgtatca
    14461 gaccctgata cttgccaatc acgcgcactt cctctgcagg gtacaccatg tcatccattg
    14521 tgggattgga tgggacgagg cgaacatgcg aaggtggctg cgggtagaac cgtttcaggg
    14581 tcgcttcgtc attgcgcacc ataaccgcgg ctatctggcc cggttccacg acctcctgcg
    14641 gacgaatgag caccaggtcc ccgggcagaa tcccgtcccc gatcatcgac tccccgttca
    14701 ccgtcaggag gaaatccccc ggccggaccg gtagcaaatc ctccagtcgt tccacccacc
    14761 tgtccgtctg ctgaattgcc tcccgcggtg aacccgcggc aattgcgcca acaaccggca
    14821 aactcagagt gtccaccatg gcaaaggcgg gaggaaggag gcgaatatgg taaggaacgc
    14881 catggccgcc gcgcgcgagt tgaatgaagc ccttgcgctc caacgcctgc aggtgctgac
    14941 gggcagtgct gtgatgcgta tacccgaaat gttgttgtac ttcggtgccc gttgctgacc
    15001 gaccttccct gtgctgaaag agtgcaagaa actgcagaat gtctttctga attgcggtga
    15061 gactggccat agtttgtgcc tcctgcgagt gaaagcgctc tggttcgctc ccggcagatt
    15121 actacactta acattaagtg tatttgaaac ggatgtcaag tgcgttggac tgcacgcgtg
    15181 gaaagagatg ttgcacaaac gatgggacgg agttcgtccg ggcagaggat gtggcgatat
    15241 ttgtttgtta gcaaccggcg ttccttccac accagagtgc cgtccgccgt attccacaat
    15301 cgggaactat gggtaacctc agacgattca gctcctttgt ccggccttac tggaagcaat
    15361 ctctggcatc cctggtgttg cttacctgtc ttgtggccat ggacctctcc atcccccgtt
    15421 tgattcagcg gatcatcgac cacgggattt accggcatga tgagcgcgtg atagttgtta
    15481 cgtcgcttgt catgctcgcg atttcagttg tcagtgcggt ctttgcggtc gggaacaacg
    15541 tgttctctgt ccgggtgagc gaaagcgtcg cgagggacct gcgcgaagcg attttccgcc
    15601 acatccagtc gttttcttac gggaatctgg acgagcagaa aacggggcag ctgatggttc
    15661 ggcttaccag cgacgtcaac gccctcaaag gcctcgtcca tatttcgttg cggatcggaa
    15721 cgcggtcacc cctcctcatg atcgggagcc tgttcctcat ggtccgtaca agccccggcc
    15781 tcgcgctgtc catggtgccg ctgctgcttc tgacctaggc caccgtaggc ttctttgcaa
    15841 cgcggatgga gccgctgttt cgcgctgtcc aggagaagct ggatcggctg aacaacgggc
    15901 tccaggaaaa cgtggccggt gttcaggtgg tcaaggcatt tgtccgggca gatcatgaac
    15961 gggcgcggtt cggggacgcc aacgaggatc tcacccgccg ctccatccgc gtgatggagt
    16021 tcatgtccac catgggcccc gtgctgacgc tgctcgtcaa cacggctgtt gtggtggtga
    16081 tctgggttgg aggacagcgg tcgatacgcg gcgatctctc gatagggcag atagttgcgt
    16141 ttacgaatta tctgatgacg acgatgacgc ctctgatcat gacggcgatg ctggcaaaca
    16201 cctgggcgag tgcaattgtc tctgcccgcc gggtgtaccg cgtactggat actgcccctg
    16261 acgtaagaga caccgaaaac gcggtttggt tgccctcctc ggccgaaggc cggatcgtct
    16321 tcgaaaacgt ttcgttccat tacggcggca gccatgacgg cgcggttctc gaggatgtca
    16381 atctcacgat cgaacctgga cagaccgtgg cgttcctggg ggcaacggga gccgggaaaa
    16441 caaccctggt acacctgatt ccccgtttct acgatgtcac ttccggccgg atcacaattg
    16501 acggcattga tatccggcag atccgccagg attcgctcct ttccgtgatg gcggtcgtcc
    16561 cgcaggagag catcctgttt tccggaacga tccgcgagaa tatacggtac ggcaagcccg
    16621 aggccgacga cgcggagact gtgcttgccg caaaggccgc ccaggcccac gatttcattc
    16681 tcgccttcga aaacggttac gacacccatg ttgaggaacg tggcgtgaat ctttccggag
    16741 ggcagaagca acggattgcc atcgcccggg cacttctcgc gcgtccgaaa atcctgattc
    16801 tcgacgacgc gacgagttcc gtggatgttg agacggaaac gaagattcac gacgcgatcg
    16861 cagcgcagca cttcgctcgc accacgctca ttgtcgcgca acgcgtaagc actgttttga
    16921 aagcggacaa aatcgtcatt ctcgatcagg gcagaatctc cgcacaggga acccatcgga
    16981 cactcatcga atccagctcg atctatcgcg aaatctgcga atcccaactg acgggcagca
    17041 ttccagttga cgacagccga gggagggcgc cggcatgatg cgcgcctcaa ctcgcgaagt
    17101 tcgcgatgtc cacgccgcca tggtaccgcg cgggccgatg ggcatgagag gcatcatcga
    17161 aaagccccgg catgcccgcg gagcgatagt gagactcctg tcctacctga aaccgtttcg
    17221 cggcgttctg gcgctcgtct ttcttctcat cgttgcgttc acgctgtgcg ggcttctcgg
    17281 gccgtatctg atgggggtgg cgatagaccg ctatatcgct acgaaggatg tatccggtct
    17341 tgccggcgtc gcagcgttga tgctgggagt gtacgtcgtt tccaacatcc tgcaggctgc
    17401 gacagggtgg atcatggcac gcgtttctca gaacgcgctg aaacaggtcc gaaacgacct
    17461 gttttcgcac cttcagagcc tgcccctgag cttttttgac cgcaaccccg ccggcgcact
    17521 catgagccgg ttgacaaacg acgtggacgc ggtgaaccag gcggtctcgc agaacattac
    17581 ctctctgttc gggagtgtgt tgtccctctt cggaatactc gtcgccatgt ttcttctcga
    17641 ccggtggctt gcgctggcca cgctggtcgt cgtgccgatc atgttcgcgt tcacccgttt
    17701 tgttgcgcgg tacacccgga aagggttccg gaggctgcaa ggtgcgctct ccgagcttaa
    17761 cagcacgatg gaggaatcca tcagcggcca caaggtggtc aaggcattcc gtcgcagcga
    17821 cgcagcgatg gagtcattca aaaaggcaaa cgacgacgtg ttccgtgccg gagtttacgc
    17881 gaacacctac gcgttgttgc tcatgcctct tacgggagtc ctcggaaatt tcttcgtcat
    17941 cgtgctggcg gggttaggcg gttggcttgc cctgcgggat ctggtcagtg tcggagtcat
    18001 cgcaacgttc atcatgtacg ggcagaattt catcaaccct ctgcggcagc tcgccaacat
    18061 gtacaattcg attcaggcag cgcttgcagg ggccgaacgg gttttcgaaa tcctcgatac
    18121 gccggcagaa gtagatgctg gagccgatgc gcccccgttt gaggaggtcc gtggtgacgt
    18181 ccgttttgaa cacgtccgtt tcgggtatca gccgggcgtt ccggttatca aggatatgac
    18241 actcgcggca aaacgaggga caaccatcgc cctggtcgga ccgacgggag caggcaaaac
    18301 cactgttatc aatcttctta cccggttcta cgaaatcgac gagggaagca tctccataga
    18361 tgacattgac attcgtagag tgcgcaaagc tgatcttcgg cgcaaactgg ggttggtgtt
    18421 gcaggatacg ttcctctttt cggatacgat catggagaat atccgttacg gcaggcctga
    18481 cgcaaaggat gaggagtgta tgtacgccgc ccggacggca gaggcggagc atttcattct
    18541 tcagttcccg caggggtatc acaccgacct taccgaacgc gcgggaaacc taagtcaggg
    18601 gcagcgccag ctccttgcga tcgcgcgcgc catcctcgca gacccttcga ttctcattct
    18661 ggacgaggca acgtccagcg tggacacccg caccgaggca cggattcaga aggcactcct
    18721 tcggctcatg gcgggacgaa cgagctttgt catcgctcac cgcttgagca cgattcgtga
    18781 cgcagaccag gttcttgtgg taaaagacgg cgaaatagtc gaacgcggaa cccattatga
    18841 actgctggag cggcgcggtt tctaccgcca cctgtatgaa agccagttca agggtgaaac
    18901 gatttgaaat gcagatggcg gccgggcatc cggccgccat ctgcggacca acttacgcat
    18961 ttgccgtgtc cgattccgat tcgctccgtt acgacggcgg cgtcaggaat cgctcaagag
    19021 cgccgcagat gcggaaatag tgtcgtccca tctggcggat tcccgtcagc atatccatgt
    19081 gggcgctgct ggtggcggcg gactcctgaa gccccgcgtc cagacgcgcg aggtggctat
    19141 cccgcaaacg tctttccagt tcttcgcctt cgtctttccg acctctgacg gcagtggcca
    19201 gcgcgcggtc tctttccctg atggcctggg cggtctgccg atgcaggtcc actgtgaatg
    19261 catggaattg gcggagttca cgcgcacctt cgtctgagaa cttcgcacca acctgcgcga
    19321 ggcgtgccga cgtgttgacc acgtccacgc cgagaaagtt ggagatctgc ctcagatcat
    19381 ccatgatagt gacatgcccc tgcttttccc tcgactgcat aagaccgagg tttttctgcg
    19441 cgaggcggat gtagtagctc tggaactggc catggagggt ttccacttcc gagcaatggg
    19501 ttgccagttt ctccctcacc gcctcgccat ctttcccgaa cgcctccatc gcgtactgat
    19561 gcatttcgcc aacgatgtca gcggtgcggt tgatttcctg ataggccgcc gtaagcgcaa
    19621 gatccggggt ttccaacagg tcgttgttca ggtatttcgc accgaacgcc ttttccttcg
    19681 tctctttgga cgggacgagc cattccacgg ccttccggaa ccagggaagg aatgggagga
    19741 atgcgcaagt ggcaccaacg ttgaagaaca tcgttccgtt cgcaacttcg cgcgcaacgg
    19801 agtttgagcc catcgccagg gtgactgcgc gcatccactg gttccagtac gtcagaatcg
    19861 ggaacacgat tgcggttccg agcacattga acagcatgtg cgcgagcgcc gcccgcctgc
    19921 cttctgtgtc ggtgcccagc gaagcgatca ccgtggtaac cgtggtgccg atgttggccc
    19981 cgaggatgat cggcatggcc gcgtccatcg tcatcaggcc ctctgaagcg agcaccacgg
    20041 cgagaccaat cgtcgccccg ctgtgcatga tcgcgctgag gatggtcgcc acaagcagtc
    20101 cgaggaacgg agcatgagcg agatacaaca tgagttcggt gaacgccggc agaattcgaa
    20161 gtggagcaac ggaactcgac attactgcca tgccgtagaa aatcatgcca aagccgagaa
    20221 agatttcgcc ggtgtagatg tacactttgc gtttggttac cagcgtcagc aaaacgccga
    20281 tacccatcat cagaagcgcg taatcactga ttttgaatgc gatgagctga acggtgaggg
    20341 tggtgccgat gttggcgccc atgatcacgc caatggcttg ggtcaagttc atcaatgcca
    20401 cgttcacgaa tccgacaacc atgacagtcg ttgcagtgct gctctgaacg accgccgtga
    20461 tgatcgcgcc ggcaacgacc gccatcaccc ggtttgtcgt gagcttcgcc agaatgtccc
    20521 tcattttgtt gccggcgacc ttctgcaaac ctcccgcgcc aagttccatc ccgaagagga
    20581 acatgccgag cccgccgaca atgcccatga caaccatgag ccaccagttg cccttcaacg
    20641 cgtgggctga aacgtgcggc atttcccctg ccatgctggc aacccgcgcc gagatgacgt
    20701 aatcaccggg tgccttgccc agggagagat ttgcggagac gaagcctgca gaatcgctca
    20761 ccaccggcga ggcgatttcc gcgtcctccg cgcccttcgg tcgcgatacg atttcgagca
    20821 ccacagaaac gccggcaacc ggaacgccag ccgtgtcggt aaccaggaaa cgcacgggtt
    20881 cttcaagaac ctgacccgcc ttgcccacaa gcaaagttcg tccaggcggg tacgatttgt
    20941 tctcgagtcg gaagggtcca ccggacgctg gagcgcccgc cgtgagcgcc gcgtttccgt
    21001 ttccctgtgt cggaacgttt gcggggtttt ctgtggagcg cagcaccatg gggttgaccg
    21061 caactcctgc gagggcaaca cacgcgagaa aaatcactgc gcgagcacgc agcgctgcgg
    21121 aagcgacagc gcgaaccata cacacctccc ccggaaaagg gtatgtcgca caaagcggga
    21181 agatcgcaac gcaatatgaa ggctgcaggt accgtcgtca atccgggtgg tctgacaata
    21241 ccctaacatt ttccgccttc acggctccac ctgtctcaca tgctgttcgc ccgggggagg
    21301 ctccttcaac catgccaggt tgaatgcgta atgattcctt gaggaaatga ggtgaatgag
    21361 tccgttgtcc gcctggcaca ccgtcatgta cccggcgact tctgattgcg acgcgctcaa
    21421 tatgttcggg cggccgtcga aggcttccac cggcctcggc aggccatcgt cgctcatcac
    21481 ccggaaattg gcccacgtcg caccttcgtc gaaggaaacc gcgcaataga ggccggtgat
    21541 cgaacggcga gtgccggtgg catcggtgat ctcaagtgga tcattcgcga atgagacgaa
    21601 aagcagcgga ccttcctgca accggaacaa caccagccgc tgtccgccgt ggatgggctg
    21661 gaaagggctg gcgctgtatg tccacgtttt tcccttgtcc tcggacaggc tcatcggcat
    21721 ccgcccgtcg atatcgtcgc ctctccccag cgccatgagg cgaccgtcgc tcagctccac
    21781 cacgttcgcg tggatgcccg cgatcgtccc cccggcgtcg taccatgtgt cgccttcgtc
    21841 ctcgctcatc cagagcgccg agccaccgtg tccggccgtg actgcgtcac agggaagtac
    21901 gattgttccg tccgaaagct ggatgacgcc tgcgatcggc atgtgccgaa ccccgtgttc
    21961 cggaatgatc agccgcgcct tcgaccacgt agcgccgcta ttgtccgacg ttcgcatcac
    22021 aactgccagc ggcccccacg tggcggcaac cgatagaccg gagaagtgaa acaacctccc
    22081 tgatttgctc ctccagagcg cacacgcgtg gtcgttgcgg tcgggagcgt cccagaacaa
    22141 ggacgccggt tcccattcat cctttcccca tcgcaggcgg ctggcaagag tcgccacctc
    22201 tctcccggcc tcttccatgc acgtgtacca catcgcgagt aggtcgccgt tcggacacgc
    22261 aacaattgcc gggacgtggt tgtgtcgcga gaacatgggt ccgtacgaat ccggaggaac
    22321 cttgacatac gtcctcgggc cgcagaaaaa tggttttgac gggtcggggc cacacgtgat
    22381 gtcccgggga atctcaggct tgacgttgct ctcgtgcagt tggatcgggc cgggtatggc
    22441 gttcgttgac ggcatttcac cgatcaccgg ccgaaatccg atgatccagc ttcgctcctc
    22501 gggaacggta cccattcgat ttgaagagcg caggtaatac aactctgtcg agtgcgaacc
    22561 acccctcgtc accctgaaat tgccatcaag cgctcccacc gggtcgttag gccggtgtgc
    22621 gtcatacggt ccgtaccaat cgtggcacca ttcttccaca ttgccgtgca tgtcgtacag
    22681 gccccacggg ttcggcggcg tctgccccac cgtcaattcg ggtatctcct gttcatcagc
    22741 cccgcgggag gggtcgggaa accatttcac tccgacgttt ttgtggaatt ccgccgacaa
    22801 ggaatccccg gtatggtacg ccgtagtcgt gccggcccga caggcgtact cccattcggc
    22861 ttccgtggga aggcggtacg gcaacccttc tcttctggaa agccactcgc agaaggcaca
    22921 agcctcgaac cagttgacaa acaccgccgc ttcgttgtcc cccgttgaga acccgagttt
    22981 gccacgcagt tggcgatggg aagggtcaaa cgcctcaaac tgagcgttcg tgacttctgt
    23041 ggttgccatt ttgaacggct tcgtcagtcg cacgagacgg atcggtcgtt catcgaagtc
    23101 cccgtaccgg cgattcggaa gcctcacgag ctcgtcggga agcggtctgt cattccccat
    23161 cagaaacgaa ccggcgggaa tatggacaaa acgcatcccg aggctgttca cgaacaccgc
    23221 atcagcgccc atcaagtcct cctcttcgtt caacccgtta ccagggtgac aaggtcctga
    23281 tactgcacga aatccagtac ggccctgccc gatgaaccac ccttggtcgt cacgtggtac
    23341 gccgcatccc agccgaaggc gatcgctcct ccaacgtctt tctcgagatt gtcgccgaca
    23401 tacaaaaccg ggctgccgga gggcacatcg ggaatgagtt cgcgtgcggc ggcaaaaatc
    23461 tccggagctg gcttgcaaac gcctatgtcg catgagcaga tgactggacc gaaatactcc
    23521 ctcaatccga cgccggcaag tgcgcgggac atcatccggc ctgtccagtc cgtgtctgca
    23581 atcacgccca tgcgcactcc cgcctcggcg agcgccttga gcgcatcggc aacaccatcc
    23641 cagagccatg tgcggtaccg cgggccacca atcgcatctg ccacgagtat ggtttcctcc
    23701 gggcggaccg gcaaactcag gcgttcgtac accccggacg ccagtctttc aaggctgtac
    23761 cgcgctccca gcttctcctg gagctcggaa ctggtatcct gcagtacccg cccgagatcg
    23821 gacggtgccc ggtgcccgaa catttccagt gccaatgaaa cacggtgcac gcggccttcc
    23881 cgaacctctc tgggcgaggg tttttctgcc agtggcccat ctctccccgg cccgaaaatc
    23941 gtgccgccac tatcccagaa aacgatctgg tatctcatca gagcaaatcc gccaccagag
    24001 gacgatggtc gctgagccgc cagacagtgc cgtctgtgtc gttttgcaca tatggttcgc
    24061 cggtgatcac gaaaaacttc ttcaatcggc gcgccactgc tggcaaaagc cagatatggt
    24121 cgcccttgcg ttccggcgag tcctccgcga catcggtttc gcccgcgttc acgtaaccat
    24181 aagatttcat cagcagatag gggtcactgt ctttgcggga gttcatgtcg ccaactacta
    24241 cgtctggcgc cggcgagcac cgaaggatcc gctccgcttc cgcgactcgc gccctgctcc
    24301 ctccctcggg agaacccgca aacctgttgc tcggaagatg cacgcaggcg atctcgaggc
    24361 tttcctcccc gtttgccacc tgtgctcgta tgtgcacccg ttcatggggc ggttgactgc
    24421 catttgacgt cagatcgcgc atcttcgaag gattccgtgt gagtattgct cctccgtaat
    24481 cggttttgac cccgcccggg gcgtggatcc aagaggccat cccgagtctc tccgcggctg
    24541 acgcgaccag actcgggtcc tgaacctcct gcaggcaaat aacgtctggc gaaagagacg
    24601 cgtacagttc gacaagccca tcgaccacgg ccggcaccgc cgccacgcgt tccacgcccc
    24661 agcgggaggg acatccctga aaccagtaga cattgtgcac cacaatgcgc atatacccat
    24721 gcctttcccg ccatgagacc gttacgcgcg accccagaaa tggaatcaga cgttgagtcc
    24781 ggacgtgagg atgttcacaa aacggtgtct ccgtgctccg atatccaccg gcgtgtccat
    24841 tccaccgcgg gcagctcgat gtcacacacg cagctcgcga ggaagatcat cccgtgaatg
    24901 cgaccggacg tcaaccaacg caatccggcc gagcattgga gtttcatgtt ctccactgac
    24961 atttcgcatt tgttgccgta gtcccacatg tagcatccaa gcactacgcg tttgccggga
    25021 gccagcgctt cgatcctttc cagattgcgt tccacgttca caaggtcttc ctgccgccac
    25081 gtccacagcg tcacaacgtc acatttgtcg agaaacggcg cgatgggcag attgagctgg
    25141 tgcgtgtaca ccacaaccca catgtccagt ttgcgcccgc actcgtccag tgtcgcgcgg
    25201 acgctctgca gcctgtcgtg ggagatcggc gcgagactgc cgtcgggttt gggatcgtgg
    25261 aagaaatcgt ccatcatcac gcccgtgatg ttcgggaact tcgccgccac gtcacgcacc
    25321 ccggtgatcg ccgcatcaga ggtgcgtccg gcctcgccga ctatggacca gacaacattt
    25381 ttgagcggca cgaacgcccg cgcgtagatg tcgtacggcg gttccggctt cccctgatat
    25441 gtcacaaaaa tcacgttcgg gatacccatg taccacgctg cttccgccgg ggtcatgcga
    25501 gacggagagg gcacccccca tccttcgttg tggctccccg cttcgtggcc ccatatccaa
    25561 agcctgtcac gaactactcc catatcgtac gcctccctgt gagtcgaatc ggtcgccaat
    25621 gccagaacct agcctttcag aatgtattcc ccgcggatga cgccaccaac tgcatacgga
    25681 acgcaatcgc aggaacgagt tgagcgttgc gctctgtgga gagctgtctt tatcatcggg
    25741 ggcagccgca acacaggcgt ctccgttttc aacaccggaa agcgcggata tggcctttct
    25801 gacatccaag caacgagttc tccgaacctt ggcgagagac gaggtggacc gtgttcccgt
    25861 tgacgtggaa cttacgggtg aaatgcggga caagctcctc tcccattttg gttttcgcga
    25921 ggacgaggag attttccggc tgtttggccg cgatttccgt cgggtaaccg cccggtacac
    25981 ggggcccgaa tgttatgcga aaaacgggca accctcggac gtctttgggg tggcaagcgg
    26041 cggcccgacg tacgccgaca cgataggctt ccgtccattt gcgagggcgg aatctgtcgc
    26101 ggaaatcgag agctactcat ggccagaccc ggactggtgg gattattctg acgttcccgc
    26161 gcagtgtgag cgatttcgag agtatgcgat taccggtggg gagtggtcgc cgtttttctg
    26221 tcaggcgtgc aacatgaccg gcatcggacg tttccttgag ttgatgctgg acgcaccgga
    26281 aatcgccgaa gcgattctct cccacatcgt ggatttctat gtcgccgggt cacggaggta
    26341 tttcgaggca gcccgcgggg gaattgacat ctttttcgtc ggagacgact acggcggcaa
    26401 aaacggcatg ctgatgagcc ccgctctctg gcgcacactc atcaagcctc aactggctcg
    26461 attgtacgcg ctcgccggcg agtatggcgt ccacatgatg caacattcgt gcgggagtat
    26521 tcggcctgta atcggcgata tgattgaact cggactggaa gtgctggatc cggtgcagat
    26581 tgcggcggcc gacatggatc cggaaggtct gaaagcggaa ttcggggacc gcctgacctt
    26641 ccacggaggt gtcaacacgg aagacacgtt gccatttgga actcctgcgg acgtgttccg
    26701 ggaaacgcgt tatcttatgg atacgctggg cgccggtggc ggttatgtgg tctgcggatc
    26761 acagtacctt cagacagaca tccccgtcgc aaacgtcatc gcgatgtaca aggcggcagg
    26821 aagtatctcc caggaaatca tttgacatcg gtgaagagag ccggacagcc gccgtgtatg
    26881 cggaggaaag tccgaacacc acagggcagg atgccgggca acacccgggc ggcgtgagcc
    26941 gatggaaagt gccacagaaa acataccgcc ggacgcgtcc tgtacgcgcc cggtaagggt
    27001 gaaaaggcag ggtaagagcc taccgcgcct tcggcaacga aggtggcagg gaaaacccca
    27061 tccggtgcaa ggccaaatag gagcggattc gaggatgccc gtctcgtcaa cacgctcggg
    27121 taggccgctt gaggagtgcg gcaacgcact tcccagagag atggttgtcc ggggagccgt
    27181 cgtccgcagg atgccggctc cttgacagaa ttcggcttac aggctctctt caccacctct
    27241 tctcaaatcc ggtgcgcaca tgcttctggc gcgctcgttg ccgtttgtcc cggaaaggag
    27301 atggttccgc gtcgctgcgt gggcggcctt tgcacttgcc gtcgtggcgc ggataggact
    27361 gcgcatacat ttcactgatt tccactcgcc tgagctctgg gaattcgggg aaattgcgcg
    27421 gaatctcgtg cagacgggta ccttcacgta tcgatccaac ggcgtcccat ccgcgttcat
    27481 gccgccgggt tacccgctct atatcgcgtt cctgtatcag gtcttcgggg ttggcgagat
    27541 cgcacacgtg atcctcgccg gcactcttct tgtcttcgaa ttgtgtctgc catttcttgt
    27601 cgggtatctt gccgccgaaa tccgggacag aactaccggt tttgttgcgt ttctcctcgc
    27661 tcttttctgg ccccagctcc tcgtcatgag cgggcgcctg acaaacatcc cgatttacac
    27721 ttcgctattg cttctggcgt gcgtcctcgt cctggccaat tcgatcccgt tacgcagacg
    27781 cgcattcctg agcgggctcg cgctcggcat gttcgggctc ttccgcttcg aagcgtgtgc
    27841 gtttctcgtt ccgttcgggt ttgttctgtt tcgccacaag aacgggtccg gctcccggcg
    27901 cgcgggcgtt ggctggcgcc gccggctggg aatcctttcc gtgttcctgg gagccttcct
    27961 catcccgtta ctcccgtgga ttggacgcaa ctacgcggtt tttggtgaag ttgtgctgag
    28021 cacgtccggc ggatacaacc ttcggcgagg gcaccacgca cgcgccacgg gaaccgcgcg
    28081 cgaacattac gccgcaggaa acactctgac cgaaagcacg gcaggcggta cgccagttcc
    28141 aggcgccgac gcgatggctc gcgtggtgga acgcactccg ggagatgttc tcgaagttga
    28201 ccgcttcttt cgcgatgaag cactgcggtt catcgcagca aatcccgcga aggaggcgag
    28261 cctggcggtg agaaagctat tcttcttcct cgtggcggac ttcacccatc cgatgtcccg
    28321 agtgttcccg atttggggta catcgtttgt ggctcttatg gtcgggctgg ttcactggtt
    28381 caggagcgga attcgcgacc cccggcagca gttcctctgg ctcgtgttcg ccacacaggc
    28441 atgcctcgca gtggtgttct tcgtggtgcc gcggtaccgc atgggcgttg agttcgtgcc
    28501 cctcgtgttt tttgctgctg cccttgaccc tcttgtccag cactggtcgg ccgattccac
    28561 cccccaacag aaagaggcgc cggtgcaccg cgcctccccc tccgaacacc cgtgacgaga
    28621 gtaaggccta aagccgcacc gtttccccgg ttttgccggc aagcagaatc gccgctgcca
    28681 ctgcaacatc ggccctgccc tcttcaccgg ggatgggaac gggtctgtca gcctgaagcg
    28741 cttcgaggaa tgcatcatcc tgctggaaca tgccgacgtt gacgttctcc gggacctctg
    28801 gaagcaccgg cttcacaatc tgcacgtcgt tgcttcccgc ggtgcgcagc gacacatctc
    28861 cccagccgca ccacgccgcg cccgtggttc ctaccacgcc acgggtgcat tcggaatagt
    28921 atgacgagtt acttgcgtct atgcggccga tggcgccttc ccccatcgtc atgacggcgt
    28981 gcacgtggtc atcgaactcg ggagaatccg ggcgcgtgta cagaacccgc cccgaaaccg
    29041 ttttcgcctc accggccagc atgcgcagca gatcaatctc gtgaacttcc cattcgatcg
    29101 ccgtaccgcc gccgattttg gggtcatatc tccaggaggg gccgtgagcc gggtcataat
    29161 agcccacgcg cgtactccag caggtaacaa ccgttccgat tttccctgag cgcgcgattt
    29221 ctatcagcgt cttcgggcca gcgtaatacc gaaggacctg tcccgccatc aactgccggc
    29281 ccgctttttt caccgccgag atcatgaaat cgcattccgg aacggtgagc gcgaggggtt
    29341 tttcgcagag aacatccgag ccggcccgtg cgcagtcctc cacgtgctgt cggtggaatt
    29401 tcggtggcgt acacactacc accgcagaag gcttgaggcg gtccagcata tcatggtagt
    29461 cggcaaatgc ggtgcccccg aactccgccg ccgccttctg cgccagactg agattcacat
    29521 ctgcaattcc cacaatttca gcgcgcgaag aaaaccgcaa cgcccgaaga tggtcttgcg
    29581 cgatagcgcc ggcacctaac acggcaacac gaacccttgc cgccataaag tcccctttcc
    29641 gaaagaaaag cccctgcggg ttacagaatc ggcaaccgaa ccgggtggcc ttctgcagct
    29701 gaaagttctg ccgcgtaaca tatctcgtgc gtttttgcgg catccaccag gttcgcgtgg
    29761 gattctacat tgagctggat acactggacg aaatgatcga tttcgtgctg gaacgggtga
    29821 tgtgtgacgt cgcctgagtc cggcaggata gtgggaattg tggcaaaacc cgtctggccc
    29881 ggcatgagct tcttcgagta gagctgattg ttccggatcg ttcctgacgt gccctggagc
    29941 gttatgggga aggtgtacgg ctgtacgcac tcgatgttcg acgccacttt cccgatcacg
    30001 ccgtccttga acttgagtac cgtcaccatg gtcggatcgt actcatagcc acccgggtac
    30061 agctcaagat tcatgctgtt cgttgaatac gcaaagactt ccactgcctc tttctggacg
    30121 aaccaccgga gtgcgtccaa tgcgtggagc cccgcggaca gcagcgaact gccgccttct
    30181 gctttgagcc ggttccacct gtactggcca taccagggcc cgatgccgtg attgtaatcc
    30241 acctcgccgt aaaacaatcg ccctatcgct tcggcgccga ggagcgcctt gatcgtctca
    30301 aaaagaggat tccacctgag gacgaaactg ataacggtct tcacctttgc cgcctcgact
    30361 gcggcacgga tttgtctgaa tccttcaaca ttcaatgcaa ccggtttctc cagaacgaga
    30421 tggcagcccg cttgtgcggc cgccaccgca tgttccgggt gcattgacgg tggggtgcag
    30481 atggagacga tgtccaagtc tgcttcatga agcattttcg cgacgtccgt gtagatcgcg
    30541 caggagaagc caaggctttt ctggtacgcc tgcgctgtct ccggccgccg gacggcgatc
    30601 gcggcaattt ccgtttcagg gttattgagg tacgcccgca tgtgttctcc ggcgacccac
    30661 ccggcaccga tgattccgac ccgatacatg gctctcccct ttccgcagaa cggccggctg
    30721 gccaaagttc cattccgcgc atcgtttcag attccggcca gaaacgacgc cgcacatcga
    30781 aagaccgaag gcgcgcacaa gtgcccgttc attcacgaga aagtatactc agcgtgcggg
    30841 agatgttcca acggaaccgg cgaagggttg ccgtgcacct agaatccggc gatcagcttt
    30901 gagagggttc tgcgggttca aaacctacgg agaaggtgct ctgaagaaga gactgcgcgt
    30961 ggcatcgaat cgccagcaga gtgctggtgc ggactatccc tggaacattc gcgatccgct
    31021 cggtgacggc ctctgcgagg gcctctgagt ctttcaccac aagaacggcg accacatcga
    31081 actggcccgt ggtagtgaac aattggcgca cctcaggaat ctcggcaagc gagcgggtaa
    31141 cggcagccac gcttttgagt tccgcattaa tcaacattat cgccgtgagc atggagttcc
    31201 tcgcgcgtgc aaaacaggcg gacagcctga tcagtggttc cggcaagaca gaaaaagggg
    31261 gcgggaccgc cccccggggt catttcttct tacgtgttct cacgccagga tccggcggat
    31321 ggcagcaatc acgtcgggct cggaaagacc atacttggcc atcagttcct cgcctgtcgc
    31381 cgattccgca tacgtatcct gaatcgcgac gaattcgatt cgggcgggat gcatacgagc
    31441 ggtcacctgc gcgacagcgc ttcccagccc tccatggacc aggtgttcct ccgcaacaac
    31501 gattcctttg gtttcttttg cggctttcag caccgcttcc tcatccaacg gcttgacggt
    31561 gtgcatgtcg agaacgcgca cgctcacgcc ctgctccgcc aggctttctg ccgcatccag
    31621 tgccggaccg acaagcatgc cgttggcgat gacagtcacg tccttgcctt cccgcacggt
    31681 gatggccttg ccgatggcga aatccgcggg cggtgcatcg tacaccaggg gagcctttgc
    31741 ccttcccgtt cgcacgaaca cggggccctt gtgctcgact gccgcgcgta ttgcctgata
    31801 cgtgcaatgc tcgtcagacg gcaccatgac gaccatcttc gggagctcac acgccagcgc
    31861 cacgtcttcc acactcatct gggatacacc gtcctcgccg atcgaaatcc ctccgtggct
    31921 gaccacgatc ttcacgttgt tttccgcgta cgcgacacca agccgaagct ggtcaaaccc
    31981 cttgcacatg agaaaacacg caaagctgga agcaaacggt attttccccg tcgtcgccag
    32041 accggaggca acgccgacga ggtttgcttc cgcgataccc acgttgaaaa acctgtcagg
    32101 gtatgccttt gcaaagtctt ttgtcatggt ggatttcgag agatcagcgt ccagcacaac
    32161 aacgttggga tggagcttac caatttcaac cagagctttc ccgtacgctt gccgcgttgc
    32221 cattcccaat gggcgcgcca tgttgtatct cctctttccg gattgattta cgacagttcc
    32281 gccagggcgc gctccatttc gtctttgctg ggcgccttac cgtggtactc gttgtcattc
    32341 tccataaacg acaccccttt gcccttggtc gtttttgcga cgatgacagt ggggcgaccc
    32401 gtttgttcct ttgcggcttc cagagcatga acgacctgat gcatggaatg cccgtcgatg
    32461 tcgagcacac gccacccgaa cgccttccac ttatccacta cggggcacac gttcatgatc
    32521 tcgtccgtcg aaccatcaag ctggtaacga ttccgatcga gaattgcgat caggttttcc
    32581 gttttgaaat gcgccgccgc catcgcagct tcccagacct ggccttcttc gatttcgccg
    32641 tcaccgagaa tgacgaatac gcggctgtcg ctaccatcca tgcgaaggcc gagcgccatt
    32701 ccaaggccta tcgagaggcc ctgcccgagt gatccggtcg aggcttcgat acccggcaag
    32761 tctttcgccg aaggatgccc ctgcagccgg ctgttcaact tgcggagcgt cagcagctcc
    32821 tctttcggga agtacccggt tcccgccatc gcggcataca cgatgggcac cgcgtgcccc
    32881 ttgctcatga tgaagcggtc gcgcttctcc cacgacgggt tctgcggatc atgccgcatc
    32941 actcggaaat aaagagccgc cacgatatcc gccgccgaaa gcgaacctcc cggatgccct
    33001 gatttcgcct catacagcat gcgaatgacg tctcgccgca attccttcgc ggttttttca
    33061 agctgggaaa cggtctgcgg agggccgtat tgagccatga aaacctcccg aaaaagacag
    33121 aaacattcct cgcggtccgt gtactcgggg cgcggaaacg aacgactcac cgactctctg
    33181 aaaatacaaa ccgctccgta ccggagccaa cgacgcgtac cagtgattcg tgactcgggc
    33241 ggtgtgtgcc gggcgtccgc cgggaagacc ggatcgcgtc gctgaagcgg tactactcca
    33301 cgggacggtt gcgcgcccgt gcgcgaaggc gctggatcag gacataccgt gcgaacactg
    33361 ccataagcct ccttttctgc cgagcgcatt ccgcgcgtac gaaacttcat gcgccggtgg
    33421 gagaggttct cctccccgct cacgacctcc gttctcactc acccgcggtc accgaatccg
    33481 gcgcagcaac tcagctccgt ccgggtggtt tcctgcgcgc aatctgcacc atgatcattg
    33541 ttcacgacgg cgccaagcat caggttcaag ttcttgtttt taagaactta taagatcgcc
    33601 attcggcccg ccttgcgtat gactctttcg aaagatttgg tgaaggagca gtttgtcgcg
    33661 tatgatgtgt cttgtatatt tggtgcattc ggcattgtgg gaaatgcccc tgactaccac
    33721 cgtaaatggt atctgtttca agagggagtt tgtgaatgac tgacccgacc ggcctcgata
    33781 gcgaactgga actccatcgt tggatggggg cgagaatccg tcagatacgt gttaccaaaa
    33841 acatgacaca ggacgaactc gcccagcgga tgggaacgca acggcctgcg gtttcgaact
    33901 gggaaaacgg gatcaacatg cccagtttgt ttacgctccg caagatggcc gcgaccttcg
    33961 gcgtctcctt gcgacaactg atcgagccgg attcggagga cgtggcagac gtaccgatga
    34021 ttcccctcga caccgcggtg gaccggccgg gcctcgaacg gtggatacgg ctgtacgagc
    34081 ggctcacgga actcggggac tccgggcagc gccttcttca gacgacgttg cgcatttttg
    34141 gtcggctcgg agctctcggt gacagcggaa gcgggcaggt agtgtattcg tcggttcttc
    34201 acgcgatcca ggaggcgctc aatttcgggc tcacggttct ctccaaggac gatttccggc
    34261 gaacgactct gttcctcagg gagaccggcc tcgcggcgga taccgaggcg gaccaggatc
    34321 ggtatgccca tctccaccag gtgttcgtgc ctccagccgg gtatgtggac cttcttgcgc
    34381 ggatgacggg cgagcaacga gtcgttgtga tcaccggtga gacccaccag ggcaaaacgt
    34441 tcgttgccac caagcttctg gccgacattg cggaacacgg gtaccgcccg atcgcggtca
    34501 tgggctgttt ttcgggactt gccggcgaca cggcacgggc tgacacacgt ctccgggact
    34561 gcctgcgtcc gggacacgcg gtgctgctgg acgatccatt ttccccgggt gcgggtgccc
    34621 tcgccttgcg cgagttcacg acaaacccca atctggcgct gagcctcgtt gccgaagccg
    34681 attgccgtct cataatcacc gcgacagcgg acgcattgga acagcgtccg gacgcggcga
    34741 gacaactggc tccgtatgaa tggtcgctcg cggacaaata cgaacccacg tccttcgtac
    34801 gaatggtgat caactacagt gagatctacc acccgggcgc gcgggaaccg attgttgcct
    34861 cacacgaggg gttatcaagc ccgcatgcgg tagagcggtc attcgcagtt gcaacggaca
    34921 acaagcagac catacgccgt agatcagcgg ggattcgccg cgaaggggta gaggcgatca
    34981 ctgtggcaga ctacgatacg ctggacaatg ccgagcttgc attcctgttg cttctgcggc
    35041 acgttccctt gcccgctcac gagctctatt cgattttcac gcgtcttgag atccagggaa
    35101 taagcggggt agtaccgacg tacgacgtgc tgttggatga cctgcggcgc tggcttctgg
    35161 tttccaaaca ggacggagca accaacatcg gcttttatca tccgtcgtac gcgtccgcac
    35221 tggacagcat tctctgggag cgcgaacggg ttcagcggct ggcgggacgc gtgttgaggg
    35281 aactcgtcac ggcaggagat ccgtacctcg atcgcgccgc gatgtcgctt ctttccgtga
    35341 tttctgtcgc aggaggggca gcgctcggca gcctgttgcg cgaggcagga cttgctgacg
    35401 gcgtccttct gaagaacgca gaccctgcca tcgccagcct catccacggc gtgatgggag
    35461 accagggcga acaacttgcg gcggatctga aaggcctgtg cgaggtttcc ttcaccaagc
    35521 ggtatttgtc gtcattctcg ctgatttccc aacaggcacg cggtgcaaac gccgcttctg
    35581 gatcacccca acaggtgtgg aacgacatgg tgaatgggta cctgtccgac aactggcagg
    35641 tgaatcgtga tctcctgaca gttctcgcca gcgccgcggt cagcggaaac gctcgaatgc
    35701 gggcactcgc cggaacactg gcgatcgttc gcggatctca acacgacgct catttctccg
    35761 cgaaactgat cgagccgttg caggacgacc gccaaaaagg ggtgacaagg tgcacaaaac
    35821 gcgcgctgct ggattacttt gacctcgtgc ccgcgagcgc tcgcgacgag ctgctggcgg
    35881 caaccgggct gtaaaccttt tgtggattgg agtgttgcgg tggaatacag aattcttgga
    35941 cgaacaggcc tgagagtaag cgaaatcggt ctgggcgctc tggaaatcgg cagagactgg
    36001 ggaattccgg taggtaccga tttcggacgc ccggacgaaa aaggcgccga acgcctcttg
    36061 aacgcggcgc tggatgccgg catcacgcta atcgacacgg cacctgcgta ccaacttagc
    36121 gaggaaagaa taggaaaggc gatcagttac cgccgccacg agttcgtgtt ggcgacgaag
    36181 gtcggcgaaa ggctcaacga cggggatgcg aaatcgcatt acgattattc ctatgaggcg
    36241 acactggcgt tcgtggagca aagccttcgg cgccttcaga cggacgtcat cgacgttctt
    36301 cagatccaca gcgcgtcaat tgaggtcatc cgggcgggag aaaccctgcg cgccatgaag
    36361 aaaatgcagg aacagggcaa ggtccggttt gtaggtatga cagggaatgt cgacgccgcg
    36421 atagaggcag tgaaaactga tgattacgac acggtgcagg tttcgtacaa tgtcgcatac
    36481 cgcgaggcgg aacacgagct gtttcctttg tgtctggaca acaacatcgg cgtgatcatc
    36541 aaggatgggc ttgcgagagg ttcgttaacc ccaaaagtgt cggcgctccc ggagaaggca
    36601 aaaaagcaga agttggtcgc cgaccggctg atttcggagt gtgttgcgaa atcccttcac
    36661 ccgaatgcct acccgaagtc gctctctgat ttcgcattac gcttcgtgtt gcaccagaaa
    36721 gcggtcacct cggtgattgc gggaacgcgg aaagtcgagc atcttctggc gaacgtggga
    36781 gcctctgacg ggcggaggct gaccgatgag atggtccgaa aagcaataga gatagttgat
    36841 tcggtggaat gagttgaccc tcgacgaaag gcactggaaa tgatcgcgtt gtctggatgc
    36901 gcggaagcat tgttcacgac acttcccttc aacgaacgaa ttgccgccat cgcggggttg
    36961 gggcttccgg cattcgagtt ctggggtttt gcgaacaagg acatccccgg gattcgtgcg
    37021 gcgatggaga aaacagggct ggctgtttcc tcgatctcct gcgatacggg aggaccgctc
    37081 gtcgacccat cgaaacggtc ggcgttcctg cctgcgtgca aaaagtcaat cgaggttgcg
    37141 cacctgctgg gctgcaagac gcttattgtc accgtggggc aagagatacc cggagtcagc
    37201 cggaaggaac agcaccggtc aattgtggat ggattgaaga ccgtggtttc gctggtcgag
    37261 gacgaagggg ttacactcgt catcgaaccg ctcaatgtcc tcgtgaatca caagggttat
    37321 ttcctggcaa ccacctcaga aggaattgac ctggtggatg aagtcgcaag cccgaatgtg
    37381 aagcttctct atgatatcta tcatcaacaa atcaccgaag gcaatctgat cgacacgatc
    37441 aaggcgaatg tcgcaaaaat tggccatttc catatggcgg acgttccagg gcgccatgag
    37501 ccgggaacgg gggagatcaa ctacgagcag gtcttccgtg cgattgccga gacttcgtac
    37561 tccgggtaca ttggtctcga gctgtggccg accaaaccag aaaaggaagc gctcgcatca
    37621 acgattgcgt gggcgaagcg ctatttcggt tagggagaga aggctctaca aaggaatgag
    37681 gctcctgccg cgtggcagca ggagcctcat ttaatttggt gcggaagggg agacttgaac
    37741 tcccacgagc ttgcgctcac tggaccctga atccagcgcg tctaccaatt ccaccacttc
    37801 cgcacaagta gaaggatttt gaaatttacc cgaatcgccg ccttccgtca accgttccga
    37861 cgtcttgtca cgtaacgaga cacggcgtta catgtccgcg attgcgccgt ccgggtggta
    37921 ccacttcacc tggtcccgtt cctcgtacgg gtgcttcgca atttcctcga gattaaggtc
    37981 aatcccgagc cccggaagtg tcggacgctt cagcctgccg ttttcgagtt cgatcacggg
    38041 cgtgtagaca aggtcacgcc atggcatgtc gtcgaaaata tgctcctgaa tgaggaagtt
    38101 gggaatgcag aaatccacct gaacacacgc ggccgtcgca acgggaccgt acgggttgtg
    38161 cggcgcggta gccatgtaat acgtctccgc catcgccgcg atcttcttcg cctccatgat
    38221 tccgccgcaa tggcagaggt caggctgaac aaccgatgca atctgccgct cgatgatctc
    38281 gcgaagtccc caccgggtat acaggcgttc accggtcgca atgggtgttt tcgcgtggcg
    38341 ggcgatcttt gccatcgctt cgacattttc cggaaggcag ggctcctcga taaacaaggg
    38401 ctcgtacgga cgcatgactt cctctgcgag gatcgccatg gaagggctca accgaccgtg
    38461 gaaatcaatc atgatatcca cctcgttccc ggcgaattcg cgaaaactgg cgatcttttc
    38521 ctccgcttca cggatcggtc tcatcgaatc aacgtaattc accgcgggaa aagggtagat
    38581 tttgaacgcg tcaatccctt tcgcaatcca ctttttgcct cgctcgatca ggctctccgg
    38641 agtcccgtcg ccaagattgc agtaaaaacg gacctcatcc cgcacatggc cacccagaag
    38701 ctcgtacacc ggaactccaa gcaacttccc cttgatatcc cagagtgcca tttcgactcc
    38761 acttatggcg ctgcagagca ccgggccacc tcgccagaac gcatgacgat acatctcctg
    38821 ccagaggtat tccacgcgcc gtgggtcctt gccgacgata tctctccgga gttcctcgat
    38881 tgcggaacgc atggtgtgct ctttgccgta cagcgag
//
LOCUS       DAOSOO010000007        38671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1092_length_38671_cov_5.621582, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000007 DAOSOO010000000
VERSION     DAOSOO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 38671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38671
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_38671_cov_5.621582"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1554)
                     /locus_tag="PLT86_01075"
     CDS             complement(133..1554)
                     /locus_tag="PLT86_01075"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT35239.1"
                     /translation="MIRPGRLTRKTAALSVGRGAGVAAQFALNMFLVRVVSQHEFGWF
                     HEIRVVLTFAALLDLGIPLGLLQVAGAAEPGRRHGLYRTALAIAGAAGLATGAILFLA
                     GLLPAFTGLSSAFAWSGLLIAATIVAAVLESALVVEERHAAIAAISATSAVGGLVLAL
                     AGVLWRPSISVAYAALALAACARAVALAKVTGVARTGVQWRPLAGAVQLLKTSIAVSG
                     HRALGIASGTIDRAVVGIFFSAGTLGWYVTGAWEVPFMSVFFSAIASAILPEMSAHWT
                     AKKNADVLAVWHRSITLSAWLIFPIWLWSWIWAPELLRVLFTRQYEDGLPVFRIYLLA
                     LPVRIAVYSALLVAMEKSRVLVNGAVIDVVVNLVLSVVLAVAIGPLGPAVATVVATYS
                     QAGYYVASVRGDLPVQRVGFFPWGSLFWALTAAGLVTLPTLVLRRVFEGDLPRAGIAL
                     AWTACILAAGAFFALRKRGTEGE"
     gene            1668..2198
                     /locus_tag="PLT86_01080"
     CDS             1668..2198
                     /locus_tag="PLT86_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOT35240.1"
                     /translation="MADETIDPRYAGCPQKHAGSSQKPNAPRPIEGVVITSIKENVDD
                     RQSLSVYLKASDPFFAGFGQSYVTKTGRGVVKAWHYHLRQTDVWYVAVGKIKVGLFDA
                     RKDSPTCGVANEVIMGGGANVTLAIPPGVFHGYLTLTEEAVLINTTNAAYDPSDEYRA
                     DWNDPRLPFVWEVVNR"
     gene            complement(2195..3415)
                     /gene="xseA"
                     /locus_tag="PLT86_01085"
     CDS             complement(2195..3415)
                     /gene="xseA"
                     /locus_tag="PLT86_01085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT35241.1"
                     /translation="MTHPEVVLGADALTVSEITAQLKELITSSFGVLWVTGEVSNFRR
                     QASGHWYFTLKDAGAQLRCVMWRSSTARLSFVPQDGMQVLARGSLDLYEAYGQHQLMV
                     SYLQPAGIGALQQAFEQLKSRLAEEGLFDNARKRPLPKWPKVVGVITSGTGAAVRDII
                     TVISRRMPTTRIIVRPAIVQGAEAAADVAAAIDEMNRHGAADVLIVGRGGGSLEDLWA
                     FNEEHLVRAIAGSKIPVVSAVGHEIDFTLADFAADVRAPTPSAAAEIVVPDQTEIRTY
                     VTGILGEMVSGMRDRVTRSADQVHRAWSADVPRRLTTRVDRMSQDVDRLVETAGIRFE
                     RLVRQRRNAFGKAAARLDALSPLKILGRGYAVCEREPDGTLITSATDVDVKDRFRVRL
                     HRGIILGKVEGRLG"
     gene            complement(3425..4303)
                     /locus_tag="PLT86_01090"
     CDS             complement(3425..4303)
                     /locus_tag="PLT86_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOT35242.1"
                     /translation="MPAQLLDGKAASAGIRREVLSRAQHLSPAPSLCAVLVGDDPASA
                     LYVRNKERACAESGIRSVFRRLPASTTQAELLDLVRELNADQDVDGILIQSPLPRGLD
                     EAGLVNAISPWKDVDCFHPENVGLLVAGTPRFLPCTPAGVQRLLILNSIETAGKHVVV
                     VGRSNIVGKPMANIMLQKTSGANATVTVCHTGTRDIGYYTRQADILIVAAGRPNTITG
                     DMVKAGAVVVDVGMNEVEDASAPRGKRFVGDVDFHAASEVASWITPVPGGVGPMTVAI
                     LMENTLRAAELRDMRR"
     gene            complement(4303..5121)
                     /locus_tag="PLT86_01095"
     CDS             complement(4303..5121)
                     /locus_tag="PLT86_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOT35243.1"
                     /translation="MRVLFVGDIVGRAGRRAAAVLIPRLRVEKNIDAVVVNAENAAAG
                     AGINPKIAEALWESGADIITTGNHAWDKREGLPLIESGRLLRPANYPSGVTGVGSMVA
                     RTKKGEALGVLCLQGRVFMKAIDCPFRTARREIESLHAETVSIVVDFHAEATSEKLAM
                     GWYLDGDVAAVVGTHTHVQTADERVLPHGTAYITDVGMTGPFDSVIGMDTKTAIQRLI
                     TGLNPRLVPAKNDVRLSGVIIDIDGATGLSTGIERVNVHYAHAGGTLSDDEESD"
     gene            complement(5134..6702)
                     /gene="rny"
                     /locus_tag="PLT86_01100"
     CDS             complement(5134..6702)
                     /gene="rny"
                     /locus_tag="PLT86_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT35244.1"
                     /translation="MDINPIVFAIGTLALVALAFVLGWVIARRVEQAKIGSAEDAAAR
                     ILDEAQREAANLKQKVQLEARNQQLEMQERNRKEADAQRADLIRMEARLVERETKLDR
                     KSDELTAREGELNGIRKALEVRQTSLSKKEEDLNRLISEQTTALERIAHMTADEAREV
                     LIANMESKARAEGARRAKEIRDRAEQDAEREAREIIGIAIQRFAGEHTTETTVSVVQL
                     PNEEMKGRIIGREGRNIRAFEMATGVDVIVDDTPEAVILSGFDPVRREVARIAMGKLV
                     EDGRIHPGRIEELVDKARNQVEEEAQRAGEEVVYELGIHDMHPQLVKTLGKLKYRTSY
                     GQNVLQHSKEVGILTGLMAEQLGLDPLLARRAGLLHDIGKAVDRENEGTHVALGLELA
                     KRYGENEEVQASIEWHHGDPVAKSIISVLVNAADTISSVRPGARRETIEGYVKRLERL
                     ESIAQAFDGVEKTYAIQAGREVRVMVKNDRVDDAMAQQLANDIAEKIQADMEYPGQIK
                     VVVIRESRSVSYAK"
     gene            complement(6837..7151)
                     /locus_tag="PLT86_01105"
     CDS             complement(6837..7151)
                     /locus_tag="PLT86_01105"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT35245.1"
                     /translation="MNGIPDSRANAITVRIYGTEYSFRSPQDPDYVKTVADTVDQEMR
                     RLSENRVVKSTLEVSVLASMHLADELLQTRKKLHEVLQRVDQTTEAMAKILDAGPSEA
                     AG"
     gene            complement(7148..7414)
                     /locus_tag="PLT86_01110"
     CDS             complement(7148..7414)
                     /locus_tag="PLT86_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35246.1"
                     /translation="MMQRNALEADVEAVLTRYDTVAREVMIQLARWASERAEWQAERE
                     ELQEALERAHERDARLQAAPLQEALQRAISRVDGILAAAEEVES"
     gene            complement(7411..9798)
                     /gene="pheT"
                     /locus_tag="PLT86_01115"
     CDS             complement(7411..9798)
                     /gene="pheT"
                     /locus_tag="PLT86_01115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT35247.1"
                     /translation="MRISLGWLNDFFPTDGLSAANIADRLTASGIEATPIGTLIPGDS
                     LVIGEVLTCEKHPNADRLSVCGVTTGGAPMTIVCGAPNVAAGQKVVIAPVGTELANGM
                     RIKRAAIRGVTSEGMICAEDEIGLGDDHEGIMVLPPDAPTGMSFLKYLHLDSERLELE
                     LTPNRPDAMSHRGVARDLCALFGRPTTNPITSPEENGRPVEEITSVLIDDPEGCPRYV
                     ARVVEGVRIAPSPEWLAGRLRQVGVRPINNVVDVTNYVLMAIGHPLHAFDLHRLAGHR
                     IVARAAERDERFTTLDGTERIIPEGSVFICDGERPVALGGIMGGLNSEITEDTTDVLL
                     EAAYFTPTRIRRTAKAVGLQTEASQRFERGADWDAVIEAIDWAAALIQQLAGGSVAKG
                     RIDAYPKRLEPAAIALRWAQIPRVLGADVPREESKRILTALGIPVRAETSEGLRCEQP
                     SWRPDLTREIDLVEEIARIWGFDQIPSRVGGVGVPPEEAPPELAIVPQLRSWCIGVGL
                     QEVITSSLVAPRYADLFCGDGAKVEITNFSTADMSVLRTHMLPSLLDVARINYSRKIE
                     GVALFELGHVYSRKAEDEYNQRRVLSILLTGSTGQVRWADQRREWDFYDLSGIAETIV
                     QRCSLRSPEIVHYDGDEFVPGTGARVLVAGKEVGQLGQVRNDVCELFDLPALAYYCQF
                     DVIGLAACQATLSRVKPLPRFPAVERDLALVVPESVESQMLENTIKEAGGALLERVIV
                     FDVYRGPGVSKGEKSVAFHLDFRAADRTLKDEEVDTFMRRIVESAQGKAGARLRE"
     gene            complement(9853..10878)
                     /gene="pheS"
                     /locus_tag="PLT86_01120"
     CDS             complement(9853..10878)
                     /gene="pheS"
                     /locus_tag="PLT86_01120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT35248.1"
                     /translation="MTAELLDELDRIAAQAPARIDACASPEEVEALRVEYVGTKGSIT
                     RINKLLREVPADQRPGLGKRVGEVRSLVLQKLQEAGQKFQVEEGARRQKIDITLPGRT
                     FLLGRRHPLTIALDRITSIFSDMGFTVADGPEIESEYYNFEALNMPPDHPARDMTDTY
                     YVTPRWPLRSQTSPVQVREMEKRRPPVRIIVPGKVYRNEDVSARAMNQFFQVEGLYVD
                     RNVTFADLKGTLETFCKRFFDPKTRVRFRPSYFPFTEPSTEVDVSCILCNGSGCRVCK
                     YAGWLEILGAGMVDPNVFGFVDYDPEEYTGFAFGMGIDRTTMMRYGVDDIRHFWENDM
                     RFLSQFE"
     gene            complement(10966..11319)
                     /gene="rplT"
                     /locus_tag="PLT86_01125"
     CDS             complement(10966..11319)
                     /gene="rplT"
                     /locus_tag="PLT86_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT35249.1"
                     /translation="MPRVKHGVASHARHKKALEHAKGYYGGRSKLFRTAREAVDRALA
                     YSYRGRKEKKRDFRSLWIVRINAAARMHGLSYSRLMYGLSQAGVDVNRRMLADLAVHD
                     PEAFGRLAETAKAQF"
     gene            complement(11387..11581)
                     /gene="rpmI"
                     /locus_tag="PLT86_01130"
     CDS             complement(11387..11581)
                     /gene="rpmI"
                     /locus_tag="PLT86_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT35250.1"
                     /translation="MPKMKSRRNARFRFKVTANGKILRHQSNCNHILTKKSSKRKRFL
                     RKSVLADAADVPRIRRMLLA"
     gene            complement(11596..12195)
                     /locus_tag="PLT86_01135"
     CDS             complement(11596..12195)
                     /locus_tag="PLT86_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35251.1"
                     /translation="MRGSVNPFIDAEGMISGAVSVVRGQKRTSANRLRVNREISALRV
                     FLVGADGVPEGEVTREEALLRASARGYDLIEVQPGANPPVCRLGKWADTAAVSPPPVE
                     AEQRTLWVPVGCTQTDFRLKIAHGRRFLTEGGVLEVVVPVEGSRDQAAAEQVLRKVVE
                     AFSDCARPGAVKRSKTELTMVFSPVTSGETRADRPDTGE"
     gene            complement(12153..14093)
                     /gene="thrS"
                     /locus_tag="PLT86_01140"
     CDS             complement(12153..14093)
                     /gene="thrS"
                     /locus_tag="PLT86_01140"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT35252.1"
                     /translation="MVRVTLPDGTPLELHDGATALDAAEKIGPRLAKATLAAKINDLP
                     VDATTALRDGDRLALLTWDSPEGRDVYRHTTSHIMAQAVKRLFPEAKPAIGPALEDRF
                     YYDFEVGRPFSPEDLEKIEAEMAEIIKEDLPVTRFELPVEEAIKLFSDRDEPYKVEIL
                     RDLSDQSELVPGEKVSLYQQGEYVDLCRGPHLPSTGRVKAFKLLSSSGAYWRGDEKRQ
                     MLQRIYGTSFPDRKQLESFLAVLEEAKRRDHRILGPRLDLFSIHPEVGAGLIHWHPKG
                     ALIRHLIETLWKEEHMRRGYSLVYTPHIASDNLYRISGHLEKYDAMYRPMDIDGENYR
                     VKPMNCPFHIMIYQSQTRSYRDLPIRYAELGTVYRYERSGVLHGMLRVRGFTQDDAHI
                     FCRPDQLVDEVSDTLDLMKFFLSTFGLDYQVYLSTRPERSFGTDEEWAVATDALKETL
                     EKAGVPYTVDPGEGVFYGPKIDVKLLDVLGRPWQGPTIQVDFQLPQRFGVEYVGSDNQ
                     THNAVMVHRAMLGSMERFVGCLIEHFAGAFPLWISPVQVVVLPLTEAQTDSATELAGR
                     LKAAGLRVETDLRSEKIGSRIRDAELQRVPYMLILGAREVENGAVAVRARERGDLGAK
                     PVDEVIRLLCEEVSTRSLTPKV"
     gene            complement(14323..>16183)
                     /locus_tag="PLT86_01145"
     CDS             complement(14323..>16183)
                     /locus_tag="PLT86_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35253.1"
                     /translation="MHLRLFASFLCAALCVACAGTHGAAPRPGPEYATPPVRLVPPPE
                     FRPDANPDRVGTILFVTVSPLYLLEKAAYFGSNGVDGIMMSGLMGSWHSDVWAANRYT
                     DDPPGTRVVGAQNSLLKLCKRMNQRCRDAGVDYNSVQVSVAQPVPDWFDDAGWARIVE
                     NFRQGAIFARDAGFAGISFDHEYISDWYLLRNPKYSEPGYPPEAEVRRMVQQRGYEIM
                     SAMLDEFPDMIFWLLPENPDNYEGWLGVDMFVGLTRGMAERDAPGGLHLCTERSYLNL
                     RPRGLATINNWTSESARFALRKAATPEVLDYWMRRCGLSVGIWPLGFYYEFKDSTGKP
                     YGYTGKRSTYPGEWVGSYADKTENYPVENFEWQIAGARSLSRRFVWLYCHGTVLWQMT
                     PDEMVHYRATPNDTLPTVWNLPEYLRILRERPVMQDPLFEATAASIREQRGFPAFAGF
                     VPRWWHVGPFPNFMENFDVVYPPERGVDLAATYAGDPSYPDSLRVLRWRESSTDAAGY
                     VDLSALFSKPGLPLAYSVAYVENPRRKRVWFRFGSNDYGRVYVNGRQVHDMREQRAAA
                     IDDDNFEVELPAGRSEILVKCGDAGGKRWGFYLRLTDAAGNEVPGLKWVGAED"
     assembly_gap    16184..16283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16837..20916)
                     /locus_tag="PLT86_01150"
     CDS             complement(16837..20916)
                     /locus_tag="PLT86_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT35254.1"
                     /translation="MRTKSLGFALLFATIALRGAANADDSFRTLTGLESSASSVAITP
                     DGQNVAAGIAGTVKVWRISDGALLSTLGQIGTEPVYGVAVTSDGGRVVAGDNGKRIRV
                     WQLSDGALLRTIATGVIPMALALTPDGEAVVAGCDDYKIRVWRLSDGQLIRTFVEQHT
                     SVVKGVAVTPDGQTVVSGSLDETIKVWRLSDGARLRTIVAGGPVWNIAATPDGQNIVG
                     SAGSLYNWIKVWSISDGSLLRTLTGHTGGVSGVAITQDGQEIVSASYDNTVRVWRFSD
                     GQLLRTLTGHTKEVFSVAVHADARTIVSGSLDLTIKAWALQSWVAGTITTTTWTAANS
                     PYRVTGTVTVPAGNTLTIEPGVDVLFDADVQFIVAGQLIANGTAQDSIRFLKGTATEW
                     GGLRFVPGDTLSSWISYARLSGANANGGTGDDDFGGAVYVTGYGTRAALARSVISGNR
                     AVAQGAGVWVGNLGSVTLVQCVLRDNVVTSAGGGGGFMANGGSLTLDRCTVVGNDAQT
                     VASVGRVANNAYATLINCVAWGNTGSPFLWDETGGFDVSFSDVEGGYPGEGNINADPL
                     FVNTATGDYNLLAGSPCIDVGDPTSPMDPDGSRADMGAFFFDKRVDQYHVGGEIADGM
                     TWSGGPYHVVGMVTVPEGATLNIAEGTDVLFDADVPFVVEGSLQALGESGEGNGIRFV
                     PGVAPEWGGLMFMGIGGPGGRTASPALERPAGVSGEGSSTIQYAEISGGDADDLSETF
                     GPMGMMGGGVSVFGTSVDIANSRIHNNQAMFGGGLAALEGGIMLDSCVVENNVANAGL
                     GGGFIAVYGGFMANDTQFRNNTAVGTSDFFVEVGGGGGYAEESYAVFERCRFTGNTSA
                     GNGGGVMLANDPVYGPPMEFKHCLFAGNTSAAGGGAYATVPMQGDKPAIRLPDSLRVG
                     LAARPAAAARAQQEPGTAFVNCTFTENTATGAESGGGALIGGKPAGTSDLLPMIYGGG
                     LCSETSIPVTSSILWGNTPDQIAMGGLGIPANKPAPYPPVAIVTFSDVQMEEGMVWEG
                     TGNINADPLFVNAAEGDFRLDVYSPCVNTGDPDGAPDPNRTRADMGAFPLEFKTGDVT
                     MDGNITGQDASKVLQFSVKLIPSIPFALADVTNNGRATALDAAMILFKWLRLSLGQDF
                     TFPLDEGYYNNAKLAIPANPRTLTWERSSSAWELRADNPEGLMAGEFAFNVGSDVALS
                     AEAGEYYAANRDGEVIRVAFARTDFTSPVLVRLVDDGRLLAPPSIASMELNDGATPIL
                     RVVRPVEFALEQNTPNPFNPTTTIRFGLPAEGQVQLVVYDITGRVVRTLVDNQMEAGV
                     HAVTWDGRDAVGRDVASGTYIYRLVAPNGVLVKRMVMVK"
     gene            complement(21358..>21650)
                     /locus_tag="PLT86_01155"
     CDS             complement(21358..>21650)
                     /locus_tag="PLT86_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35255.1"
                     /translation="LKQSGSRCIEMTVACANAEIPRSAQNDGVVHRCISIQRPGISTG
                     SMTGQPPAFSDFARRHRRPKFGRRVAAEAKRKPLYRDDRGLRQRGDYSLRTE"
     assembly_gap    21651..21750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21751..21969
                     /locus_tag="PLT86_01160"
     CDS             <21751..21969
                     /locus_tag="PLT86_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35256.1"
                     /translation="ISIQRLPLCFSRHSTTVLATAERIGEIIPHVAHPQETGENWIPA
                     CAGMTTSVLVRRRGGARDGMGEILRCSE"
     gene            22401..23342
                     /locus_tag="PLT86_01165"
     CDS             22401..23342
                     /locus_tag="PLT86_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35257.1"
                     /translation="MRANTDWFRDAKWGVFMHWLAGMHRPGCTVNEWNAMVDDFDVNG
                     LANQLAEAGAGYLFFTVGQIDGFYACPNAAYDRIVGITPSKCSRRDLVSDLSDALATK
                     GVRLLVYLPAEPPYLDAEAVAKFPWVREPKGRRQAEFQVMWEGVIRDFALRWGTKIWG
                     WWFDGCYYATDMYRHPEPPNFASFAAAAKVGNPDALVAFNQGVYTPVKPQSAFEDYTA
                     GEISDAFPVWQGYDNLPGRFVGGAQYHILSYLAGKWGYDDPRFPDEFVIGYTKHVNQH
                     EGVVTWDVPNTPQGLIRKPFMNQLIALGNALGTIRHK"
     gene            23353..24183
                     /locus_tag="PLT86_01170"
     CDS             23353..24183
                     /locus_tag="PLT86_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT35258.1"
                     /translation="MTRYIAAYDTESPRCIKGVREIVRVHRKYSMPATFYITGTTAEG
                     NPDEYRELLDDPLFEVASHTYSHKMLRDNPFCGPAQPPEGIREQILRGKDVVEKVFKR
                     DCLGLRPGCSFEFGLKGAPDVLAVVKEGGFKYVSSMAWGKDYSLPAPLNQPFPYEEDG
                     YPDIWEIPCHGWHENLLKNHNKWGPRRITLWPPEMPEAIPTDFLKTPEEEFAVNKVFL
                     DKARNDALSFVSLIWHPWSLYAFDPEMRMLELTFSYVQSVGLAPTTYAGFCRELNAAK
                     "
     gene            24233..26788
                     /locus_tag="PLT86_01175"
     CDS             24233..26788
                     /locus_tag="PLT86_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOT35259.1"
                     /translation="MDTSVSRRLLQTIDCTGEFGPDRYFSHGIASVVETDAGRYREAE
                     GVPGARFAYRFAIEHVGRPHEVVIQYPDDKRRFMCVMDGTCYDLTTGVLTGGCQPLSR
                     KMLELRQVFWPRWKDCSIVFMTWGEGEPAAAARIEVFELDDLPPMRVAGDSAYGPFRE
                     IGVQYEDPCGTCASEGALNRDEWTDRIVAYMKHTGQQSLTYPLAWYHGPTFPSDREPS
                     SQLDWIVAPDRTCYSRWTSHPVDWYQGLLQRFSDEGLTFRGSLTMLRLGSLMAGMQVD
                     EEAIHRGADTYNTVLWNGTVRAGTGDWTPMYDARFMSELAKKLDDAPEYEPLNSGQPG
                     YPPFLYGERPNPDGGGPVFNPLHPTVQTAVLGFVREIAERYAKFPAFTGISFNYFAAQ
                     MLWFGSLRAGYDDYTISLFEKETGLKVPGDAASPDRFFQRHLYLTTRCRTAWIEWRCV
                     KIRDLMRQIRDTLREVRPDLTVTLTLWQETAVPFLFSGMHAGTQLGARPGAYEFYREG
                     GVDWDMFRHEPGILLDLEMGDSRDRGGHGANPAAGVNTPLEVDCMFRDHAYLDTCSLE
                     AAGRQDLPGAFLFNCWVEAWGRYLWFPVEPDDSNLTVVSDMDGQRPDAIVRSHSEYPA
                     DGFWWEPQMRITPALKGGGHFLEGFCHALAELDACRITQGGLFLDTAHTEELRRFASV
                     FRSLPNEKFETVGHTTDPVAVRSLVKDDRRFVYFVNREYYPVAVTVAFNQAPGNVINA
                     ATGESEPGLSPWMVVLSPYELRAIIVAPEIALTGFEARPPAEIASVLEQRAREALADI
                     ESVRLSGVLVPGMDEMEEGISRAMEEKRYAWLRRALDGYIVRKCRALAADRSE"
     assembly_gap    26821..26920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26921..28146)
                     /locus_tag="PLT86_01180"
     CDS             complement(<26921..28146)
                     /locus_tag="PLT86_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35260.1"
                     /translation="MPTKQDIAIIRELARQKAEIAALPVQEEKRALWRKLNGLKPDRP
                     MVAIDQVCWNEMNVNDELTLRCADPELRGYEDRLRRELFQWRHFPGDMVVDPYIHVSK
                     AIHNTGFGVRVQERVAVTDPTSGVVGHKYTNQFETEEDLQKIQIPRLTHDKAATARRL
                     EFAHDLFDGIIDVRLWGMDAYLSLWDPISTWMSVESALLAIVDRPDYVHRLVGRMTDG
                     YMAMFDQAEAEGLLCGPQNWVHCTGAFTDELPAPGYNPEKPRTKDMWAMGLAQMFSTV
                     SPKIFKEFEVDYVSRLCARFGLVYYGCCDPLDGKMNEVRMIPNVRKVSMSPWVNEERG
                     AAEIAGDYVYSRKPSPAFLAHDSFDAEAVRSDLQKTRDICERYGCPLEYILKDISTVR
                     YQPQRLFKWSEVAMEVA"
     gene            complement(28303..30000)
                     /locus_tag="PLT86_01185"
     CDS             complement(28303..30000)
                     /locus_tag="PLT86_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOT35261.1"
                     /translation="MDLDLARTPFSRYGSYFVFSYLDRPKTRQPGLYLRTVRGGNPTH
                     DLLRVELLVDGIPAPFTINANTTILTLEAGPASVSFCIAEQTAIRIKGNGAGVRFTHA
                     PIAAFDNVFLVDSATAEVNDWSSGIRLAISVATGSLNADAPWQNPRCTHMVIDCLPDS
                     GDGVLDCVVEEFKTTRPLRNRPCPTFDECTKSVAAEFRKWAKRSPKVSMVHENAAELA
                     QYLTWSSVVAPSGRLNRPAMLMSKNWMSNIWCWDACFNAMALAYRNPELAWDQFMLFF
                     DHQDQNGALPDSLNDVSIVWNFTKPPVHGWALRWMMERSDFITRRELKQVYAPLAAWT
                     NWWMTARDDDGDGVPQYNHGNDSGWDNATVFDVGLPVESPDLSAYLVIQMDVLSEIAD
                     ALGRGRDARRWKKRADTLLAKMISHSWRDGQFVAPRSGQHDVAEGDCLLPFVPIVLGK
                     RLPKEQRKALVAGVLRDGRFLTEHGLATESPASAKFDPKGYWRGPIWAPATLLIVDGL
                     VALGERGAARQISKRFCDMVLAEGMAENFHPHNGKGLCDPAYTWTASVFQILAHEYV"
     gene            complement(30200..31507)
                     /gene="melA"
                     /locus_tag="PLT86_01190"
     CDS             complement(30200..31507)
                     /gene="melA"
                     /locus_tag="PLT86_01190"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT35262.1"
                     /translation="MPKITFIGAGSLGFFKTLMIDCLSFEALRNSDWALVDIDPKRLD
                     YARRIGERIMRDAGSTGKLVATLDRREVLKGSDFVIMAILVGGYEPIAFDIDIPMKYG
                     VDQCIGDSHGPGGVFRALRTIPVKVGIARDIADLCPNAWVLNYTNPMSMLCRAMDIET
                     GINLVGLCHSVQGTTGMLADWAGVPRDEIDYWVAGINHQAWVLRIRHNGEDLYPKIKE
                     AARKPEHWNSLTASCEMLEHLGYFVTEGSGHNSEYNAWFRKTPESVAKYCPGGSWNGG
                     SGFIKQLYGSDREGWEADQERFASDTSPIVFRRSHEYGSNIINGIQTHVPFRFNGNVA
                     NKGLITNFPQDVMVEVPCFADKNGVKPCHVGALPPQLAALNSMNINVIELAVQAALKG
                     DRDAAFHACCYDPLTMAVLDLRQIRSMVDEMFEAEKPWLPQFA"
     gene            31876..33084
                     /locus_tag="PLT86_01195"
     CDS             31876..33084
                     /locus_tag="PLT86_01195"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT35263.1"
                     /translation="MNVLVINCGSSTIKYQLINTDDQQALAAGSVERIGMADAVLSHQ
                     PQGREKVRVTGEILDHIQAIEYTLAILLSPQYGVIRDTSEIDAVGHRVVHGGEKFSGS
                     VILTDEVMAQIRECIELAPLHNPHNVRGIEACQRLMPKVPEVAVFDTAFHQTMPDYAY
                     MYALPWKLYERHAIRRYGFHGTSHGWVAARTAELLGRPISSLKMVTCHLGNGCSIAAV
                     DGGKSVDTSMGFTPLEGLVMGTRSGDIDPAIILHVIGKEDLNVHEATALLNKHSGMLG
                     LSGVSGDMREIEAQVEAGDPKATLALRVYCYRIRKYIAAYAGAMGGLDTIVFTAGVGE
                     NSSLVRKLSCEGLTFMGVELDDTLNGTRSKTGRVISTDTSRVKVVVMPTNEELAIALE
                     TARLVAPLRQ"
     gene            33282..35180
                     /locus_tag="PLT86_01200"
     CDS             33282..35180
                     /locus_tag="PLT86_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35264.1"
                     /translation="MTAYSSLYLDSSLLGDDYLPIGAFSLFLGLLAINASLRRTGWGL
                     TRSDVHTAYIMMLVSAAVPVNGFAARVLPMITGLYYYATPANGWEEYFFRHVPNWIGP
                     RDRDVARTFYEGTITGNVPWESWLKPLAVWFIPFAGVALMVLALGIFLRRQWIENERL
                     VFPLAQVAVAVTGPGNTPTIETSLIRNKLFWVAVLVPFAINALNGLHHLYPVIPNIAA
                     MDPDLKTSLGGYLTDPIQQYMCRDFYFEFHFALIGVAMMMRQEVSLSLWFFQVFFVGV
                     IFVFMVTGLGPGQYMYTPKEQFGYVMFARWTKIGAAVVVVAVILWSLRHEFVRAWRAA
                     FGRRPVSEEGQAPLGFAFWMFVAGLAAYAGWTRALGLPPGAALAMLAITLGAYIVVAR
                     IVSEGGLIWCSVAMDPVLIYPTLVGTSGLDGQTITAMAYTGFVPLAARANVLPSVMDS
                     FKIAQGSRIRPSHVIIVSLASLALAFVVSLVVVLAMTYAQGGNYMPQKHYSYGPRWIF
                     GKAASFFTDVTGPSWQAIATMIAGIAIMAGMYKLHRRYLWWPFYPLGFVIAETEVMQH
                     QWMSIAIGWAIRSLVIRFGGAAGYRSLRPAAIGVIIGELTSIMFWFGVFALTGTTGKS
                     LTHATSTW"
     gene            35353..37431
                     /locus_tag="PLT86_01205"
     CDS             35353..37431
                     /locus_tag="PLT86_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35265.1"
                     /translation="MAEENAGNQPEMNVGLSAIRERHRGHSVEDRTVPPQTGSPWRGL
                     TLGIVGVVILSIVTPYADAYMESSSLSATYLPTGVVLLFLLILGMNTLLRYTRFPFTQ
                     RELMLAYVTMLVPSAMPADGWSLRLVPLLIELDYYATPVNEWKILHGSNIPHWMTPHG
                     NEVVTWFFTGRPRGARIPWEPWVRPLAIWCVLAAGLYLIMLSFSIAFRKRWMDAERLQ
                     FPLAQVPLTIMGDDPAPSWVSSFFRQPLLWAGMAVPFFFHTINGLNLYFPAIPSIRLT
                     DIFFSTIFAGSQFITEPPFAEWAGVRLNFYWSIIGISYLLRSEVSLSVWAFEWFYNFE
                     MIVFQWSGYGNGQFEWSPLHTFGYSLMARYQRAGAIAAASIAYFVASRKEIGEMVRAA
                     FGMRTTRYRGGEAAIPWWSFWAFVVGMTIYFTWTAATGMKTIAAVVLLAFFLGIAITT
                     ARIVAASGLLWVYDFFVPMHGLTVLVGTARIDPKSFTNVGYVDFVALNNRAVIMPQIL
                     DGMKISRQTGIRQSHFFLGIVLGTVVATVVSMATVLWLGYTYGGANMTDFMFRGGGEW
                     LFNRVAGFQRYRVYTNWTVIGCMSAGAAFMMSLLYLHRTYLWWPLYPLGFIIGGSVAS
                     GQMWFPVMLGWLAKAIIVRFAGAGAYDRFKATALGLVLGEFLCVGMWMAIDAATGLTL
                     HRVFPVWTPQ"
     gene            37456..>38671
                     /locus_tag="PLT86_01210"
     CDS             37456..>38671
                     /locus_tag="PLT86_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35266.1"
                     /translation="MAQHENEPSEPVKPAEHGPDLEAVRERHEARSNMQSALAQENQT
                     IHTGAPWRGLTLGLFGVVILSVVTPYADAFLQSSGLAATYLPTGVFLLFLFALGINTA
                     LRFTRMAFTQQELMLAYVTMLVPSAIPSDGLATRLVPLLVELYYYATPINEWAILHQP
                     NVPHWMTPQGQEVIDWFFTGKPTGAAIPWEAWVKPLALWILLAAALCLIMISLSVAFR
                     KRWMDAERLQFPLAQIPLMVMGDDPTPSWYSSFFRQPLVWAGIAIPFFFHTVNGLNIY
                     FPSIPPIGLANITLGSLFSGSRVFTDVPFYRWANIQFNFYWSIVGISYLLRSEVSLSV
                     WVFEWFYYIEHIIFESTSIGDGQFQWTPLHTFGYTLAARYHRLGAMVLAMLVYFIASR
                     GEIREVLLAAFGQ"
BASE COUNT         7402 a        11752 c        11282 g         7935 t
ORIGIN      
        1 ccgctgaaga gggggaatac tgggcgcgca ggattgttgc cgcacttatc gcgaaaggcc
       61 gcgacctcct gcctgagttc gccgggggaa gcacctcggt ctgaatgcgg cgcattcccg
      121 cacacgtgcg gcctattctc cttccgtgcc ccttttgcgc aaggcgaaaa acgctccggc
      181 ggcgagaatg caggccgtcc atgcgagtgc aatgcctgcc ctcggcaggt caccctcgaa
      241 cacgcggcgc aggacgagag taggcagtgt tacgagtccg gcggccgtca aagcccagaa
      301 gaggcttccc cacgggaaga aaccgacccg ctgcactggc aggtctcccc gcacgctggc
      361 gacataatac cccgcttgcg aataggtagc caccacggtg gcgactgcgg ggcccagcgg
      421 gccaatcgcc acggccagta ccacactcaa caccagattg actaccacat caatcactgc
      481 cccgttcacg agcacccttg atttttccat tgcgacgagc agggcggagt acaccgcaat
      541 gcgcacgggc aacgcaagca ggtagatgcg gaacaccggg agtccgtcct cgtactgccg
      601 cgtgaaaaga acgcggagca gttccggtgc ccagatccac gaccagagcc agatggggaa
      661 aatcagccac gcggaaagcg tgatcgaccg gtgccagacc gcgaggacgt ccgcgttctt
      721 tttcgccgtc cagtgcgcgc tcatctccgg gagtatcgcc gacgcgattg cgctgaaaaa
      781 caccgacatg aagggcactt cccacgcccc cgtgacgtac cagccaaggg tgcccgcgga
      841 gaagaagata cccaccactg ctctgtcgat ggtccctgac gcgataccga gtgcgcgatg
      901 gcctgacacc gcgatgctgg ttttcaggag ctggaccgcg cctgccagcg ggcgccactg
      961 aacaccggtc cgtgcgacac cggtaacctt cgccagcgcc acggcacgcg cacaggccgc
     1021 cagagccagc gcggcgtacg cgacgctgat cgaaggccgc cagagaactc ccgccagcgc
     1081 aagaaccagt cccccgacgg cgcttgttgc cgaaatcgcg gcgattgcag cgtggcgttc
     1141 ttccaccacg agtgcagatt cgagcaccgc ggcaacgatc gtggcggcga tgaggagtcc
     1201 cgaccaggcg aatgcggagg acagccccgt aaaagcgggg agtaaacccg cgaggaaaag
     1261 aatcgcgccg gtcgccagac ctgctgcgcc cgcgattgcc agggcggtcc ggtagagacc
     1321 gtgccggcga ccgggttccg ctgcccccgc cacctgaagc agaccgagag gaatgcccag
     1381 atcgagaagc gcggcaaacg tgagtacgac tcttatttcg tgaaaccagc cgaattcgtg
     1441 ctggctgacg acgcgaacca ggaacatgtt cagcgcgaac tgcgcggcca cacccgcacc
     1501 gcggcccacg ctgagcgcag ccgtcttccg cgtcaaccgc cctggacgga tcataccggc
     1561 accaccaggc agttcgggga gcactccgtt ttcgcgttgc ggggcgattt cgttatcttg
     1621 aacaacatgc gttgtccccc gctgaggtgc cacggaagga aataacgatg gccgacgaaa
     1681 caattgaccc acggtatgcg ggttgccctc agaaacatgc gggcagttcg cagaagccga
     1741 acgccccgcg ccccatcgaa ggggttgtta tcacttcaat aaaggaaaac gtggacgacc
     1801 gacaaagcct ctctgtgtac ctcaaggcgt ccgacccctt ttttgcgggg ttcggtcaga
     1861 gttacgtcac caaaaccggc cgcggcgtag tgaaggcgtg gcattaccat cttcgccaga
     1921 cagacgtctg gtacgtggca gtcggcaaga tcaaagtcgg tctctttgat gcgcggaaag
     1981 attcgcccac gtgcggcgtt gcaaatgaag tcatcatggg aggcggggcg aacgtgacgc
     2041 ttgcgatacc cccgggagta tttcacggat acctgacact cactgaggaa gccgtgctca
     2101 tcaacacgac caatgcggcg tacgaccctt ctgatgagta ccgcgcggac tggaatgacc
     2161 cccggttgcc tttcgtgtgg gaggtggtca accgttagcc gaggcgtccc tccactttcc
     2221 ccagaatgat gccgcggtgg aggcgcacgc gaaaacggtc tttcacatca acatcggtcg
     2281 cactggtaat gagcgttccg tccggttcgc gttcgcagac cgcatacccg cgcccgagaa
     2341 tcttgagcgg gctcaaggca tccaaccggg cagcggcttt cccgaacgcg tttcgtcgct
     2401 gtcgaacaag gcgttcaaac cggatgcccg cggtttccac caaccggtca acatcctgtg
     2461 acatccgatc cacccgcgtc gtcagccggc gcggaacatc ggcactccac gcgcggtgca
     2521 cctgatctgc cgagcgggtt accctgtctc tcatgcctga aaccatctct ccgagaatac
     2581 cagtgacata cgttcggatt tcggtctggt caggcacaac gatttcggcg gcggcggagg
     2641 gagtcggcgc tcgcacatcc gccgcaaagt ctgcgagtgt gaaatcgatc tcgtggccca
     2701 ccgccgacac gaccgggatc ttcgagccgg cgatcgcgcg aaccaggtgt tcctcgttga
     2761 aggcccagag atcttcgagg gaacctcctc cccgacccac gatcagaaca tccgctgcac
     2821 cgtgacggtt catttcgtca atcgccgccg ccacgtccgc cgctgcttcc gctccctgca
     2881 cgatcgccgg acggacgatg atgcgcgtcg tcggcatccg acggctgatt acggtgatga
     2941 tatcccgtac cgccgctccc gtcccggaag tgatgacgcc gacgactttt ggccatttgg
     3001 gcaggggccg ttttcgcgcg ttgtcgaaaa gcccctcctc cgctaggcgc gatttgagct
     3061 gctcgaacgc ctgttgcaga gccccgatgc cggccggctg aaggtaactc accattaact
     3121 ggtgctgacc gtaagcctcg tacagatcca agcttccacg agccagaacc tgcatgccgt
     3181 cctgcggcac gaatgagagc cttgcggttg aggagcgcca catcacgcac cgcagctgcg
     3241 ctcctgcgtc tttgagcgtg aaataccagt gcccggacgc ctggcgtcgg aagttgctga
     3301 cttccccggt aacccagaga acaccgaaag atgacgtgat gagctctttg agctgcgcgg
     3361 tgatctcgct tacggtgagc gcatccgcgc caagaacaac ttcggggtgt gtcatcacct
     3421 ggggtcagcg cctcatgtcg cgcagttcgg cggcacgcaa tgtgttttcc atcagtatcg
     3481 ccaccgtcat cgggccgact ccccccggca caggggtaat ccagctcgcg acttcactcg
     3541 cggcatggaa gtccacgtca ccaacgaatc gtttcccgcg cggtgccgat gcatcctcga
     3601 cctcgttcat gccgacgtcc acaacaaccg cacccgcctt gaccatatcg ccggtgatcg
     3661 tgttcgggcg ccctgccgcg acgatgagga tgtccgcctg ccgcgtgtaa tacccgatat
     3721 cgcgggtgcc ggtatggcag accgtaacgg tcgcgttcgc tccggacgtc ttttgaagca
     3781 tgatgttggc catcggtttg ccgactatgt tggaacgccc caccaccacg acgtgcttgc
     3841 ccgcggtttc aattgagttc agtatcagca gcctttgcac accggcgggg gtacacggaa
     3901 ggaaccgcgg cgtaccggcc accagtagac ccacgttctc cggatgaaag cagtccacat
     3961 ccttccacgg cgagatcgcg ttcaccagcc ccgcttcatc caacccacgc ggcagtggcg
     4021 actgaatgag gataccgtcc acatcctgat ccgcgttgag ttcgcgcaca aggtcgagaa
     4081 gttccgcctg cgtcgtgctg gcaggaagac ggcggaacac ggaacgtatc ccggactccg
     4141 cgcatgcgcg ttccttgttt cggacataaa gcgcggacgc agggtcgtca cccacaagga
     4201 cggcgcacag gcttggggcc ggcgacagat gttgcgcccg tgagaggact tcacgtcgga
     4261 tgccagcgga ggcggctttg ccgtcaagca gttgcgctgg cattaatctg actcctcatc
     4321 gtctgaaagt gtaccacctg cgtgcgcata atgcacgttc acacgttcta tgccggtgga
     4381 aagaccggtt gcgccgtcaa tgtcaatgat cactccagaa agcctcacgt cgtttttggc
     4441 ggggacaagc cggggattca aacccgtgat cagacgctgg atggccgttt ttgtgtccat
     4501 tccgatcacc gaatcaaacg ggcctgtcat cccgacatcc gtgatgtacg cggttccatg
     4561 tggcagcacg cgctcgtctg cggtctgaac atgcgtatgc gtaccaacta ctgcagccac
     4621 gtcaccgtcg agataccagc ccatcgcaag tttctccgaa gttgcttcgg cgtgaaaatc
     4681 caccacgata gataccgttt cggcgtgcaa ggattcgatc tcgcgcctgg cggtccggaa
     4741 cgggcagtct atggccttca tgaagacccg cccctgcagg cataagacgc ccagcgcctc
     4801 gccttttttt gtgcgtgcca ccatcgagcc cacgccggtg accccgctcg ggtaattcgc
     4861 ggggcggaga agacggccac tttcaatgag aggcagcccc tctcgtttgt cccaggcatg
     4921 gttcccggtc gtgatgatgt cggcacctga ttcccagaga gcttccgcaa ttttcgggtt
     4981 gatccctgca ccggccgccg cattttcggc atttaccacc acggcatcaa tgttcttttc
     5041 aacccgaagg cggggaatca gcacggcagc ggcacggcgc cccgcccggc cgacgatatc
     5101 ccccacaaaa agaacccgca tgcggaacgg tcctcacttc gcgtaggaaa cggaacggga
     5161 ctcccggata acgacgacct tgatctggcc ggggtattcc atgtccgcct gtattttttc
     5221 ggcgatgtcg ttggcaagtt gctgcgccat cgcgtcgtcc actcggtcgt ttttcaccat
     5281 cacccgaacc tcacgccccg cctgaatggc gtaggtcttt tccacgccat caaatgcctg
     5341 ggcgatcgat tcgaggcgct ccagccgttt cacgtaccct tcgatcgttt cccttcgcgc
     5401 gccgggccgc acactcgaaa tcgtgtctgc cgcgttaacc agcacactga ttatggactt
     5461 cgcaacagga tcaccgtggt gccattcgat actcgcctgg acttcctcgt tttcgccgta
     5521 tcgtttggca agttccaatc cgagagcaac gtgcgtgccc tcgttttctc tgtcgacggc
     5581 ctttcctatg tcatgcagca ggcctgccct cctggcaagc agaggatcca accccagttg
     5641 ctctgccatc agaccggtaa ggattcccac ctcttttgag tgctgcagaa cgttctgtcc
     5701 gtaactggtg cggtatttca gtttgcccag cgtcttgacg agttgagggt gcatgtcgtg
     5761 gatgccgagc tcgtacacaa cctcttcacc ggcacgctgc gcttcttcct ccacctggtt
     5821 ccgcgctttg tccaccaatt cctctatacg cccggggtgg atgcgcccat cttccaccag
     5881 ttttcccatg gcaatcctgg ccacttcgcg gcgcacggga tcgaaccccg aaagaatcac
     5941 cgcctccggt gtatcgtcca cgatcacatc cacgccggtt gccatctcaa acgcgcgaat
     6001 attgcgacct tcgcgcccga taatccgtcc cttcatctct tcattcggca actgaaccac
     6061 agaaaccgtg gtctcggtcg tgtgttcccc ggcaaagcgc tggatagcaa tcccgatgat
     6121 ctcccgtgct tccctctccg catcctgttc tgcacgatca cggatttctt tcgcacgccg
     6181 cgcaccctcc gcgcgggcct tgctttccat gttggcgatg agcacctctc gtgcttcatc
     6241 cgcagtcatg tgagctattc gctcgagcgc cgtcgtctgt tcggagatga gccgattgag
     6301 gtcctcttcc tttttcgaca ggcttgtctg acgtacttca agcgctttcc ttatgccgtt
     6361 gagttcgccc tcgcgggcag tgagttcatc gcttttgcgg tcaagttttg tttcccgctc
     6421 caccaggcgg gcttccattc tgatcaaatc tgctctctgg gcatctgcct cttttcgatt
     6481 gcgttcctgc atctccagct gctgattgcg ggcctcgagc tggactttct gcttcagatt
     6541 cgcggcttcc cgctgcgctt cgtccagaat tcgcgcagca gcgtcttccg cgctgccgat
     6601 ttttgcctgt tccactctcc tcgcgatcac ccacccgagt acgaaggcga gagcgaccag
     6661 cgcaagagtt cctattgcga acacgatcgg attgatatcc atcccctgta tcctccggga
     6721 aacaactttg cccgagtgcg aaaataaaaa acccctgttc cgtcatgcgc gcatggcaac
     6781 agcacgcgct ttcgcggaag agggggttca acgagtggcg ctcggccccg aggctgtcaa
     6841 ccagccgctt ctgaaggccc cgcgtcgagt atcttagcca tcgcctccgt cgtttggtcc
     6901 acgcgctgca acacctcgtg cagtttcttt cgcgtctgaa gcagttcgtc ggcaaggtgc
     6961 atgctcgcca gaaccgacac ttccagcgtg gacttcacaa cccggttttc actaagacgg
     7021 cgcatttcct gatcgacagt atcagcaacg gttttgacgt aatcggggtc ttgcggcgaa
     7081 cggaacgaat attctgttcc gtagatacga acggtaatcg cattcgccct ggaatccgga
     7141 atcccgttca tgactccacc tcttctgccg cagccagaat gccatctacc cgggaaatcg
     7201 ctcgttggag cgcttcttgc aacggtgccg cctgcaaacg tgcatccctc tcatgagctc
     7261 gttcgagtgc ttcctgcaac tcctcgcgct ccgcctgcca ctccgcccgt tcgctcgccc
     7321 agcgggcgag ctggatcatc acttcacggg ccacagtgtc gtaccgcgta agaacagcct
     7381 ccacatcggc ttcaagagca tttcgttgca tcattcccgc aatctggctc ctgctttccc
     7441 ctgcgctgat tccacaatgc ggcgcatgaa cgtgtctact tcctcgtcct tcaacgtcct
     7501 gtccgctgca cggaaatcga gatgaaacgc gacactcttt tcacccttgc tcacgccggg
     7561 gccccggtat acgtcaaaca caatgacgcg ctcaagcaac gcgccgcccg cctctttgat
     7621 agtgttctcc agcatctgtg attcgaccga ttccggaacc acaagcgcca gatcgcgttc
     7681 cacggccgga aagcgaggaa gcggcttcac gcggctcaga gttgcctggc acgccgccaa
     7741 accgataaca tcgaattggc agtaatacgc caatgcgggg aggtcgaaaa gctcacacac
     7801 gtcattccga acctgcccca gttgacccac ctctttgccc gcaacgagaa ctcgtgcgcc
     7861 cgtacccggc acgaactcgt caccgtcata atgtacaatt tccggcgaac gaagagaaca
     7921 gcgctgaaca atcgtttcgg cgatcccgga aaggtcgtag aaatcccact cgcggcgctg
     7981 atcagcccag cggacctgac cggttgaccc ggtcagcaga atacttaaca cccggcgttg
     8041 attgtactca tcctcggctt ttctgctgta cacgtgcccg agttcgaaaa gtgctacccc
     8101 ttcgattttg cggctgtagt tgattcgcgc aacatcgagc aggcttggaa gcatgtgagt
     8161 tcggagcacg ctcatgtcgg cagtggagaa attcgttatc tcgaccttcg ctccgtcgcc
     8221 gcagaaaagg tcggcgtacc ggggcgccac caggctgctc gtgatgacct cctgaagccc
     8281 cacaccaatg caccatgacc gaagctgtgg cacgattgcg agctccggcg gagcctcttc
     8341 gggaggcacg cccacgccac cgactctcga cggaatctgg tcaaaacccc agattctggc
     8401 gatttcctcc accaggtcaa tttcccgcgt caagtccggt ctccaggagg gttgctcaca
     8461 ccgaagcccc tccgaagtct cggcgcggac cggtatgcca agagccgtga ggatccgttt
     8521 cgactcctcc cgcggaacat ctgcgcccag cacacggggt atctgagccc accggagcgc
     8581 tatcgctgcc ggttccagcc ttttcgggta cgcgtcaatt ctcccctttg caaccgagcc
     8641 accggcaagc tgctgaatca gcgccgcggc ccagtcaatc gcttcgataa ctgcgtccca
     8701 gtctgcaccg cgttcgaaac gctgtgatgc ctctgtctgc agccccactg ctttcgcggt
     8761 tcgacggatg cgcgttggag tgaagtacgc cgcctcaagc agcacatccg tggtgtcctc
     8821 agtgatctcc gaattcagcc cccccatgat tccgcccagg gccacgggtc tctccccgtc
     8881 gcagatgaac acgcttcctt cgggaatgat tcgctcggtt ccatcaagcg tcgtgaatct
     8941 ttcgtcgcgt tccgcagccc gagcaacgat ccggtgtccc gccagccggt gaagatcgaa
     9001 cgcatgcaac gggtggccaa tcgccatgag cacgtagttg gttacatcca ccacgttgtt
     9061 gatcggccgg acgccaacct gccgcaaacg gccagccagc cattccgggg agggtgcgat
     9121 ccttacccct tcgacaacac gcgccacgta ccggggacac ccttcagggt catcaatcag
     9181 aaccgaggta atctcttcca ccgggcggcc gttttcttcc ggcgaggtaa tggggttggt
     9241 tgtcgggcga ccgaaaagcg cgcaaagatc cctcgccacg ccccgatggc tcatcgcgtc
     9301 gggacggttt ggcgtcaatt cgagttcaag ccgctcactg tccagatgga gatacttgag
     9361 aaacgacatg ccggtcggag catccggagg gaggaccatg atgccctcgt ggtcgtcacc
     9421 gaggccaatc tcatcttccg cgcagatcat gccttcagac gtgacaccgc ggattgcggc
     9481 ccttttgatg cgcattccgt tggcgagttc cgtccctacc ggtgcaatga cgactttctg
     9541 gcctgctgcc acgttcggcg cgccgcatac gatcgtcatg ggagcaccgc cagtcgtcac
     9601 gccacatacg gacaggcggt ccgcgttcgg gtgcttttca cacgtcagca cctctccgat
     9661 cacaagggaa tcccccggaa tcagtgtgcc gatcggagtg gcttctatgc cggacgcagt
     9721 caggcgatca gcaatgttcg ccgcggacaa cccgtccgtt ggaaagaagt cgttcagcca
     9781 accaagtgag attctcatgc tctcgcgcct tcgtcaagtt gacccctgtc gcccttcacg
     9841 cgtccaccgt tctcattcga actgggacag gaatcgcata tcattttccc agaaatgcct
     9901 gatatcgtcc accccgtacc gcatcatcgt ggtgcgatca atgcccatgc cgaacgcaaa
     9961 cccggtgtac tcttccgggt catagtccac aaaaccgaat acgttaggat ctaccattcc
    10021 agcgcccagt atttccagcc acccggcgta tttgcacacc cggcatcctg aaccgttgca
    10081 gaggatgcag ctcacgtcca cttccgtgct gggttccgtg aaagggaaat acgacggccg
    10141 gaaacggact ctggttttcg ggtcaaagaa ccgtttgcag aacgtttcca gtgttccctt
    10201 gaggtccgcg aacgtgacgt tccggtcaac atacagccct tccacctgga agaactgatt
    10261 catggcccgg gcgctgacgt cctcgttacg gtataccttg ccgggaacaa tgatgcgcac
    10321 cggtggacgt cgcttttcca tttcgcgcac ctgcaccggc gaagtctgcg acctcagagg
    10381 ccatctcggg gtcacgtaat aggtgtccgt catgtcacgc gccggatgat ccggaggcat
    10441 attgagcgct tcgaagttgt agtattcgct ttcgatctcc ggcccgtccg ccacggtgaa
    10501 ccccatgtcc gagaaaatcg acgtgatacg gtcaagggct atcgtgagcg ggtggcggcg
    10561 gcccagaaga aaggtccggc cgggtaaagt aatgtcgatc ttttgcctgc gggcaccctc
    10621 ttcgacctga aacttctggc cggcctcctg caacttctgc agtacaagcg aacgcacctc
    10681 ccccacgcgt ttgccgaggc ctggccgctg gtcggcaggc acttcccgaa ggagtttgtt
    10741 gatgcgcgtg atactgccct tcgttcccac gtactccacg cgcaacgctt ccacttcttc
    10801 gggcgacgca cacgcgtcaa tccgtgcggg ggcctgtgcg gcaatcctgt cgagttcgtc
    10861 aagcaattcc gctgtcatga tgcaaaaaag gcggacgttc tcgtcaggag cgcatcgttc
    10921 cgcgcgctct tgacaaggcg ttgtccgcct ctccttccgt ggaggttaaa actgcgcttt
    10981 tgcggtttct gccagccggc cgaacgcctc gggatcgtgc accgccaggt ctgcaagcat
    11041 gcgccgattc acgtctacac cggcctgcga cagcccgtac atgagccggg aatagctcag
    11101 cccgtgcatc cttgctgctg cgttgatgcg aacgatccag agagaacgga agtcccgttt
    11161 cttttctttc ctgccgcgat acgaataggc cagtgcgcgg tccaccgctt cacgtgcggt
    11221 gcggaacagc ttcgaccgcc cgccgtaata ccctttcgca tgctccagcg ctttcttgtg
    11281 acgtgcgtgc gacgccactc catgtttgac ccgtggcatg ttttgactcc gtgtgataat
    11341 cgctgcggcc acctgcccgg tggaaacagc tcccgtatga aactgattac gcgagcagca
    11401 tccgtcggat gcgtggcaca tccgctgcgt cagcaaggac gcttttgcgc aggaaacgct
    11461 tgcgcttgct cgatttcttg gtaagaatat gattacaatt cgactggtgg cgcagaatct
    11521 ttccgttcgc ggtcaccttg aaccggaacc gagcgttccg gcggcttttc atttttggca
    11581 cagcttgctc tccctctatt cgccggtatc aggccggtcc gcgcgggttt cacccgacgt
    11641 gaccggcgaa aaaaccatcg tcaattccgt tttgcttctc ttgactgctc ctggcctggc
    11701 gcaatctgaa aaagcctcga ccactttgcg caacacctgt tcggcggctg cctggtcccg
    11761 cgaaccctca accggaacca caacctccag aacgccacct tcagtcagaa atcggcggcc
    11821 gtgggcgatc ttcagccgga agtcggtctg cgtgcagccg accgggaccc ataacgtccg
    11881 ttgttcagct tctacaggtg gcggactgac cgccgccgta tcggcccact tgcccaaccg
    11941 acacaccggc ggatttgccc cgggctggac ttctatgaga tcgtatccgc gtgccgaggc
    12001 tcgcaaaagc gcttcttcac gcgtgacttc gccttccggc actccatcgg ccccgacgag
    12061 aaacacccgg agagccgata tttcccggtt tacgcggagt cggttggcgc ttgttctctt
    12121 ctggccgcgg acgacagaaa cggccccgga aatcatacct tcggcgtcaa tgaacgggtt
    12181 gacacttcct cgcacaggag gcgaataacc tcatccaccg gctttgcgcc cagatcgcct
    12241 cgttcccgcg cacgaacggc aacggccccg ttttccactt cgcgggcacc aaggatgagc
    12301 atgtacggta cgcgctgcag ttccgcgtcg cgaatccgcg atccgatctt ctcgctccga
    12361 aggtcggttt caacgcgcaa cccggcggct ttcaaccgcc ccgccagctc cgtcgccgag
    12421 tcggtctgcg cctcggtaag gggcaacacg acgacctgca ccggggatat ccacagcggg
    12481 aacgcaccgg caaaatgctc gatcaggcag cctacgaacc gctccatgga accgagcatg
    12541 gcgcggtgca ccatgaccgc attgtgtgtc tggttatccg accctacgta ctccacgccg
    12601 aagcgttggg gaagttgaaa atctacctga attgttggcc cttgccaagg tctgcccagt
    12661 acatccagca atttgacgtc tattttgggc ccgtagaaca cgccttcacc cggatccacc
    12721 gtgtagggaa cgcccgcctt ctcgagcgtc tccttcagtg cgtcagtggc taccgcccat
    12781 tcctcgtcgg ttccgaaact ccgctcgggc ctcgtgctca ggtagacctg gtaatcgaga
    12841 ccaaacgtgc tgaggaaaaa cttcatcagg tcaagggtat cggagacttc gtccaccagt
    12901 tggtccggcc ggcagaaaat gtgcgcgtca tcctgcgtga agccgcgtac acgcaacatg
    12961 ccgtgcaata ccccggaccg ttcgtaccgg tacaccgtgc ccagttccgc gtatcggatt
    13021 ggtaggtcgc ggtaactgcg ggtctgcgac tggtatatca tgatgtggaa cgggcagttc
    13081 attggcttga cacggtagtt ctcgccgtca atatccatcg ggcgatacat cgcgtcgtat
    13141 ttctcaaggt gaccacttat ccggtacaga ttgtcgctcg caatgtgcgg agtgtacacc
    13201 agggagtacc cccgccgcat gtgctcttct ttccacagag tctcgatcag gtgccggatc
    13261 aaagcgccct tcggatgcca gtgaatcaac ccggcaccga cctcaggatg aatgctgaaa
    13321 aggtccaggc gcggacccag aatccggtga tcacgcctct tggcttcttc cagcacggca
    13381 aggaaactct caagctgttt cctgtccgga aagctggtgc cgtaaatgcg ctggagcatc
    13441 tggcgttttt cgtcgccgcg ccagtacgcg ccggaggaac tcagcagttt gaacgccttt
    13501 accctcccgg tgctgggaag gtggggaccg cgacagaggt ccacgtactc gccctgctga
    13561 tacaggctca ctttttcccc gggaaccagc tcactctggt cggagagatc gcgcagaatc
    13621 tccaccttgt agggttcgtc tcggtcgctg aagagcttga tcgcttcctc gaccggcagc
    13681 tcaaaccgcg taaccggcag gtcttccttg ataatttccg ccatctccgc ttcgatcttt
    13741 tccagatctt ccggcgagaa cggtctgcca acctcgaaat cgtagtagaa acggtcctca
    13801 agtgctggac cgatcgcggg tttggcttca ggaaacagac gtttcaccgc ctgtgccatg
    13861 atgtgcgaag ttgtgtgccg gtagacatcc cttccctcgg gggaatccca tgtcagaagg
    13921 gcaagccggt ctccgtcgcg aagagcagtg gtggcatcga ctggaagatc gttgattttc
    13981 gccgcaagcg tggccttggc aagcctcgga ccgattttct ccgccgcatc gagggctgtt
    14041 gcaccgtcgt gaagctcgag gggtgtgccg tcggggagag tgacgcgaac catatttgaa
    14101 ctcctttccg tacgaacagc caacgccctg ccacagacga ccgcagccct cgcgtcagcc
    14161 atccacagat ggaaacgccg gcgagcgcac tcacatggtg aggcacctca ccggcgcatt
    14221 gcgtgcttcc tggcgatgcg acgtaccacc gtctggaagc gagaacacaa gaattattaa
    14281 gataccccca cggcacgata tttgccaaac cgccgaaggt atctaatcct cggcgccaac
    14341 ccatttgaga ccgggcactt cgtttcccgc ggcatcagtg agacgcagat agaaccccca
    14401 tcgcttgcct ccggcgtccc cgcatttgac gagaatctca gaacgtcctg ccggcaactc
    14461 cacctcaaag ttgtcatcat cgatggcggc cgctcgctgc tcacgcatgt cgtgcacctg
    14521 ccggccgttc acgtacacgc ggccgtagtc gttgctcccg aaccgaaacc agactctttt
    14581 cctgcgcggg ttttccacat aggcaacgct gtaggcaaga ggcagacccg gtttgctgaa
    14641 aagcgcggag aggtccacgt aaccagcggc gtcggtcgag gactcgcgcc agcgcagaac
    14701 acgcagcgaa tcgggatatg aagggtcgcc cgcgtaggtg gcagcaagat caactccgcg
    14761 ctccggcggg tacacgacat cgaagttctc catgaagttc gggaacggcc caacatgcca
    14821 ccagcgtggt acaaagcccg caaacgcggg aaatcctctt tgttcacgta tcgaggccgc
    14881 cgttgcctca aaaagaggat cctgcataac gggacgttcc cgaagtatgc gaaggtattc
    14941 cggcagattc cagacagtcg gcagcgtgtc gtttggcgtg gcgcggtaat ggaccatttc
    15001 atccggcgtc atttgccaca gaaccgtccc atggcagtag agccacacaa aacggcgtga
    15061 gagcgagcgc gcacccgcga tttgccattc gaagttctcc acggggtagt tctctgtctt
    15121 gtccgcgtac gagccgaccc attcgccggg atacgtggat cgtttacccg tgtagccgta
    15181 cggtttccct gtggagtcct tgaactcgta gtagaacccg agcggccaga tacccacgct
    15241 gagcccgcaa cgtcgcatcc agtaatcgag cacctcaggc gtggcggctt ttcttaacgc
    15301 gaatctcgca gactcgctcg tccaattgtt gatcgtggcg aggccgcgcg ggcggaggtt
    15361 caggtacgac cgttccgtgc acagatgcag ccctcccggc gcatcccgtt ccgccatgcc
    15421 gcgagtgagg ccgacaaaca tgtcaacccc gagccagcct tcgtagttat ccgggttctc
    15481 cggaaggagc cagaatatca tgtccgggaa ctcgtccagc atggccgaca tgatctcgta
    15541 ccctcgttgc tgcaccatcc tgcgaacttc ggcttcgggc gggtaccccg gttcgctgta
    15601 tttcgggtta cgaagcaggt accagtcgga gatgtactcg tggtcgaagg atatcccggc
    15661 gaacccggcg tcacgggcga agatcgcgcc ctggcggaag ttctccacaa tcctcgccca
    15721 tccggcgtcg tcaaaccagt ccggcacggg ttgcgccaca gagacctgaa cactgttgta
    15781 atccacaccg gcatctcgac atcgctggtt catccgtttg cagagcttca ggagggagtt
    15841 ctgtgctccc acgacacggg tgcccggagg gtcatccgtg tagcgattgg cggcccagac
    15901 gtcactgtgc cagctcccca tcagtcccga catcatgatg ccgtccacgc cgttggaacc
    15961 gaaatacgcg gccttttcca ggagatagag gggcgatacc gtcacaaaca ggatggtgcc
    16021 aacgcggtca gggttcgcgt ctggacggaa ctcgggaggc ggtacgaggc gaaccggcgg
    16081 cgtggcgtat tccggaccag gtcggggagc cgccccgtgc gtccccgcgc acgctacaca
    16141 gagggcagcg cacagaaacg acgcaaaaag cctcagatgc attnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnngggcggt ggtggagctg gtggattagg ctggtgcccc
    16321 gcttggaacg cggggcacca gctggattcc cgcctgcgcg ggaatgacgg aatcgaagcc
    16381 gcaaacgccg aggccaccct acctgaaaga aacgcaaccc ctttttgacg gacgcgagcg
    16441 cgttacggaa caggactcgg aatggtgttt gtaattccga ttccgtagtc gggtcattcc
    16501 cgcgtaggcg ggaatccgga cgggaaggcg cacgggtctc ctcgatacag cgctgcggcc
    16561 gcgttgcgga ccttcgcggc acccaggaac cggaccccgc ggtcatctcg atacaacgcg
    16621 acgggtgcaa agccccgtcg cgccactcga tgaccgttca tcgccattcg gattcggaag
    16681 agcacgccgg gcggcagtcg atgaccggcc attgcctttc gggcgttggg gggcaagtag
    16741 gaagcactcg atgaccagtc atggcgccgc ggctccaaaa aagagtgcgg ggtgagcgtt
    16801 gccgcccacc ccgcactcca cgcgttggtt cctcgcttat ttcaccatca ccatgcgctt
    16861 gacaagcacg ccgttcggtg caaccaggcg gtagatgtag gtgccgcttg caacgtcacg
    16921 gccgaccgcg tcgcggccat cccatgtcac ggcatgcacg ccggcttcca tctggttgtc
    16981 caccagcgtc cggacaacgc gcccggtgat gtcatacacc acgagttgca cctgcccctc
    17041 agccggcaac ccgaagcgga tggtcgtcgt cgggttgaac gggttgggcg tgttctgctc
    17101 cagcgcgaac tccaccgggc ggacgacacg cagaatcggt gtcgcgccgt cgttcagctc
    17161 catggaggcg atggatggcg gcgcgagtaa cctgccatcg tccacgaggc ggaccagcac
    17221 cgggctcgtg aagtccgtac gggcaaacgc cacgcggatg acttcgccat cgcggttggc
    17281 cgcatagtac tcgcctgcct ccgcggagag ggcgacgtcc gaccccacgt tgaaggcaaa
    17341 ctcacccgcc atcagtcctt ccgggttgtc cgccctcaat tcccacgcgg agctagaccg
    17401 ctcccacgtg agcgtgcgcg ggttcgccgg aatcgccagc ttggcattgt tgtaatagcc
    17461 ttcgtccaga gggaacgtga agtcctggcc gagactcaac ctgagccatt tgaacagaat
    17521 catcgccgca tccaacgcgg tcgcacggcc gttgttggtc acgtcggcca gcgcgaacgg
    17581 gatactggga atcagcttca cgctgaactg gagcaccttt gacgcgtcct gacccgtgat
    17641 attcccgtcc atggttacgt cgccggtctt gaactccagc gggaacgcgc ccatgtcggc
    17701 acgggtacgg ttggggtccg gtgccccgtc aggatcgccc gtgttgacgc aaggcgagta
    17761 cacgtcaagc cggaaatcgc cttccgcagc gttcacgaag agcgggtcgg cgttgatgtt
    17821 ccctgtgcct tcccagacca tcccctcttc catctgcacg tcgctgaacg taacgattgc
    17881 gacgggcgga tagggagcag gtttgttcgc cgggattccc agaccgccca tggcaatctg
    17941 atccggcgtg ttaccccaca ggatcgagct ggtaaccgga atgctcgttt cagagcagag
    18001 accgccgccg tagatcatgg gaaggaggtc ggacgtccct gcgggtttgc ccccgatgag
    18061 cgctccccct ccggactctg caccggttgc cgtgttttcc gtgaacgtgc aattcacgaa
    18121 tgcggtgccg ggttcctgtt gcgctcgcgc agcagcagcc ggcctagccg ccagaccaac
    18181 cctgagggaa tccggcagac gaattgccgg tttgtccccc tgcatcggaa ccgtcgcgta
    18241 cgcgccgcca cctgctgcag aggtgttccc cgcaaacagg cagtgcttga actccattgg
    18301 tggtccgtag accggatcgt tggccagcat gaccccgccc ccgttgccgg ctgaggtgtt
    18361 ccctgtgaac ctgcaccgtt cgaataccgc gtacgattcc tccgcatatc cgccgccgcc
    18421 gccgacttcc acgaagaagt cgctcgtacc cacagcggtg ttgttcctga attgggtgtc
    18481 attcgccatg aaaccgccgt acacggcgat gaaaccgccc cccaaaccgg cattcgccac
    18541 gttgttctcg acgacacacg aatccagcat gatgccgccc tcgagcgccg cgagtccgcc
    18601 gccgaacatg gcctggttat tgtggatacg tgagttggcg atatctacgg aagtgccaaa
    18661 aaccgaaacg cccccgccca tcatacccat cggaccgaac gtttcagaga ggtcatctgc
    18721 atccccgccg ctgatttcgg cgtactgaat tgtagacgag ccctctccac tcacaccggc
    18781 gggccgttca agcgcaggac tcgccgttct ccccccaggc ccaccgatac ccatgaacat
    18841 caacccaccc cactcaggcg ccacaccggg cacaaagcgg atgccattcc cttcacccga
    18901 ttcaccaagc gcctgcagcg agccttccac aacgaacgga acgtccgcat cgaacagcac
    18961 gtccgtacct tcggcgatgt tcagcgttgc gccttcggga accgtcacca tccccacaac
    19021 gtggtacggg ccgccgctcc acgtcatccc atcggcgatt tcgccgccga cgtgatactg
    19081 gtcaacccgt ttgtcgaaga agaacgcgcc catgtcggcg cgcgaaccgt ccgggtccat
    19141 ggggctcgtc ggatcgccca cgtcaatgca tggcgagccc gcgagcaggt tgtagtcgcc
    19201 ggtcgcagtg ttcacgaaaa gcgggtcggc gttgatgttg ccttcaccgg ggtaaccgcc
    19261 ctcgacgtcc gaaaaagata cgtcgaaacc accggtttcg tcccagagaa aggggcttcc
    19321 ggtgtttccc cacgcgacgc agttaatcaa tgtcgcgtag gcattgttcg ccacgcggcc
    19381 aacgctggcg acggtctgtg cgtcattacc gacgacggta caccgatcga gggtcaggct
    19441 tccgccattg gccatgaatc cgccaccgcc gcccgctgaa gtcaccacgt tgtcgcggag
    19501 cacgcactga acgagggtta cggatccgag gttgccgacc cacacgccag cgccctgcgc
    19561 gaccgctcgg ttcccactga tcacgcttct cgccaatgcc gcgcgcgtgc cgtatcccgt
    19621 cacgtacact gcaccaccga agtcatcgtc acccgtgccg ccgtttgcgt tcgcaccaga
    19681 taggcgcgca tagctgatcc agctcgagag agtgtcaccc ggcacgaacc gcagaccgcc
    19741 ccactctgtc gccgtgcctt tgagaaaccg gatgctatcc tgcgcggtgc cgttcgcgat
    19801 cagttgcccc gccacgatga actgcacatc cgcgtcgaac agcacgtcca cgccgggttc
    19861 gatggtgagc gtgttaccgg caggaaccgt gaccgtgccc gtcacgcggt agggggagtt
    19921 ggcggccgtc catgtggtgg tggtgatggt gccggcaacc cagctttgga gcgcccacgc
    19981 cttgatggta aggtcaaggc ttcctgacac gattgttcgc gcatccgcgt gaacagcgac
    20041 gctgaacacc tcctttgtgt gtccagttaa cgtgcgcagt agttggccgt ctgagaatcg
    20101 ccatactctc acggtgttgt cgtaacttgc tgacactatt tcttggccgt cctgcgttat
    20161 agcgacgcca gatacgccgc ccgtgtggcc cgtgagtgtc cgcaataagg aaccgtcaga
    20221 aatgctccac accttgatcc agttgtacaa gctcccggcg cttccaacga tgttctgacc
    20281 gtctggcgtg gcggcaatgt tccagaccgg cccccccgct acgattgtcc gcaaacgcgc
    20341 accgtccgaa aggcgccaca ctttgatagt ttcgtctaag cttccagaaa ccacggtctg
    20401 tccgtcgggt gtaacggcaa cgcctttcac aacactcgta tgctgttcta cgaacgttcg
    20461 aattagctgg ccatccgaaa gccgccaaac tctgattttg tagtcatcgc accccgcgac
    20521 gaccgcttca ccatctggcg tgagagctaa ggccatgggg atcacgccag tcgcgatggt
    20581 tctcaggaga gcaccgtcgg aaagctgcca caccctgatt ctcttcccgt tgtcgcccgc
    20641 aacaactctc ccaccgtcag acgtcacggc aacgccgtaa acgggttcag ttccgatctg
    20701 cccgagcgta ctcaacaacg cgccgtctga aatgcgccaa accttgactg tcccggctat
    20761 tcccgcggca acgttctggc catcaggggt gatggcgacg ctgctcgccg agctctccaa
    20821 tcccgtgagc gtccggaacg agtcgtccgc gttcgcggcg ccgcgcaacg caatggttgc
    20881 gaagagaaga gcaaaaccga gggatttggt gcgcatggtg gttccccctt ccccgcatgg
    20941 tgcggggtgg cgcgatgcgt tcgcttagaa aaggcaaagg ctctcgaata tccggcagat
    21001 tgaagagcgc cgtgggcggg tcagggcggt cgtgcggcgt ctcggacgca tgcatccacc
    21061 acctgccacc tgcgcacgcc ccgacccgtg cgcgagcgga aaaaactcgg atggataaca
    21121 taaccggttc acgagtcaat aataccttgt ttatggcgcc tgtcaaatag gactgttttt
    21181 cgaacgcacc gggcggcgga gatgccgctc agaagcgcgc aatttcggcg tttctgcccg
    21241 gcggcgtacc cggattgaac tttttttgcg aaaacgcttg acacgaggac gccgcgttgc
    21301 cagtgtgtga atatggccgg gcgaggttgt acccagacgc atcggtcgac gacgccgtca
    21361 ttctgtgcgg agcgagtaat ctccgcgttg gcgcaggcca cggtcatctc gatacagcgg
    21421 cttccgcttt gcttcagccg ccactcgacg accgaacttg ggccgcctgt gccgtcgcgc
    21481 gaagtcactg aacgccggtg gctggccggt cattgagcct gtcgaaatgc cgggacgctg
    21541 tatcgagatg caccggtgaa ctacgccgtc attctgtgcg gagcgaggaa tctccgcgtt
    21601 ggcgcaggcc acggtcatct cgatacagcg gcttccgctt tgcttcagcc nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atctcgatac agcggcttcc gctttgcttc
    21781 agccgccact cgacgaccgt gcttgcgacg gctgaacgga ttggtgagat aataccccat
    21841 gtcgcgcatc cgcaagaaac gggagaaaac tggattcccg cctgcgcggg aatgacgacg
    21901 tcggtgttgg tgagaaggag gggcggggcc agagatggaa tgggagagat tcttcgctgc
    21961 tcagaatgac ggaatgtgac acctgctacg gtcatctcga tacagcggct tccgctttgc
    22021 ttcagccgcc actcgacgac cgaacttggg ccgcctgtgc tggtcgcgcc gagccactgg
    22081 acggcggtgc ccggccggtc attgagcctg tcgaaatgcc ggcccgatgt ttcgagatgt
    22141 accggtgaac tacggcgtca ttctgagcgc agcgaagaat ctctgcgttg gcacaggaca
    22201 cggtcatctc gatacagcgg cttccgcttt gcttcagccg cgggcgcgga gaccacgccc
    22261 ctacgctggt gtgcgccgaa cgccactcgg agaccgtacg cggcgcgcct tgcgtagccc
    22321 gcttgcccct gaggaggaat gggcggcatt cttagcactc gtggacacag tggattggtt
    22381 ccaaccgccg ggaggcacag atgcgggcaa acaccgactg gttccgtgac gcgaaatggg
    22441 gtgtgttcat gcactggttg gcagggatgc accgtcccgg ctgcaccgtg aatgagtgga
    22501 acgccatggt ggatgatttc gacgttaacg ggctggcgaa ccagcttgcc gaagccggag
    22561 cggggtatct gtttttcacc gtggggcaga ttgacggctt ctacgcctgc ccgaacgcgg
    22621 catacgaccg gatagtaggc atcacgccgt cgaagtgctc gcgccgggac cttgtgagcg
    22681 acctttcaga cgcgctggcg acgaagggtg tccggctgct ggtgtatctg cccgcggagc
    22741 cgccgtacct cgatgcggag gcggtggcaa agttcccgtg ggtgcgcgaa ccgaaagggc
    22801 gaaggcaggc ggagtttcag gtcatgtggg aaggagtgat ccgcgatttc gcgctgcgct
    22861 gggggacgaa gatctggggc tggtggttcg acggctgcta ctacgccacc gacatgtacc
    22921 gccacccgga gccgcccaat ttcgcgagct tcgcggcggc ggcgaaagtg ggcaatccgg
    22981 acgcgctggt agcgttcaac cagggggtgt acacgccggt gaagccgcag tcggcgttcg
    23041 aggactacac tgcgggtgaa atcagcgatg cgtttcctgt ctggcagggg tacgacaacc
    23101 ttccgggccg tttcgtcggc ggggcgcagt atcacatcct ctcctacctt gcggggaaat
    23161 ggggatacga cgaccctcgc ttccccgacg agtttgtcat cgggtacacg aagcacgtga
    23221 atcaacacga gggcgtggtg acgtgggacg ttcccaacac gccgcagggg ttgatcagaa
    23281 aaccattcat gaaccaactg atagcgctgg gcaacgcgct tggaaccatt cgacacaagt
    23341 gaaagacaca ccatgacacg ctacatcgcg gcgtacgaca cggaatcacc acggtgcatc
    23401 aagggagtcc gggagattgt gcgcgtgcac cggaagtact cgatgccggc gacgttctac
    23461 atcaccggca cgacggcgga aggcaatccg gatgaatacc gggaactgct cgacgacccg
    23521 ctgtttgagg ttgcgtcgca tacctactcc cacaaaatgc tgcgggataa cccgttctgc
    23581 ggcccggcgc agccgccgga ggggatacgg gagcagatcc tgcggggcaa ggacgtggtt
    23641 gagaaggtgt tcaaacggga ttgtctgggg cttcggccgg gatgcagttt tgagttcggc
    23701 ctcaagggcg cgccggatgt gctggcagtg gtgaaggaag gcgggttcaa gtatgtgagc
    23761 agcatggcgt ggggaaaaga ctacagcctg cctgcgccgc tcaatcagcc gttcccctac
    23821 gaagaggacg gttacccgga tatctgggag atcccctgcc acggctggca tgagaacctg
    23881 ctcaagaacc acaacaaatg gggtccccgc cggattactc tctggccgcc ggagatgccg
    23941 gaggccatcc cgacggactt cctcaaaacg ccggaagagg aattcgccgt caacaaagtc
    24001 tttctggaca aagcaaggaa cgatgcgctg tcgtttgtgt cgctcatctg gcatccgtgg
    24061 tcgctgtatg cgttcgatcc ggaaatgcgg atgctcgagc tgacgttttc gtatgtgcaa
    24121 tccgtggggc ttgcaccgac cacgtacgcg ggattctgca gggaactcaa cgccgcgaaa
    24181 tagcgaggtc ggcgctggct ttgttttggc gccaatcagg agtggggagt gtatggatac
    24241 ctccgtttcg cgtcggctcc tgcagacgat cgactgcact ggagagttcg ggcccgatcg
    24301 gtatttctcc cacgggatcg cttccgtcgt cgaaactgac gcgggacggt accgggaggc
    24361 ggaaggggtg cccggggccc ggttcgcgta ccggttcgcc atcgagcacg tgggacggcc
    24421 gcatgaggtg gtgattcagt accccgacga caagcgccgg ttcatgtgcg tgatggacgg
    24481 aacctgctac gacctcacga caggcgtgct gacgggtggc tgtcagccgt tgagcaggaa
    24541 aatgctggag ttgcggcagg tgttctggcc tcggtggaag gactgcagca tcgtcttcat
    24601 gacatggggc gagggagagc cggccgcggc ggcacgaatc gaggtatttg aactggacga
    24661 tctgcccccg atgcgagttg cgggtgattc ggcgtacggg ccgttccggg aaatcggcgt
    24721 gcagtatgaa gacccctgcg gaacgtgcgc aagcgagggt gcgctcaaca gagacgagtg
    24781 gacggaccgt atcgtggcgt acatgaaaca caccggccag cagagcctca cgtatccgct
    24841 ggcgtggtac cacggcccga cgtttccctc cgaccgggaa ccgagctcgc agttggactg
    24901 gattgtcgcg ccggatcgaa cgtgctactc gcgctggaca tcccatccag tggactggta
    24961 ccaaggcctg ctccagcgct tttcggacga agggctcacg tttcgcggct cgcttacgat
    25021 gttgcggctg ggaagcctca tggcgggcat gcaggtggat gaggaggcga ttcatcgcgg
    25081 cgcggatacg tacaacacgg tgctgtggaa cgggaccgtc cgagcgggca cgggcgactg
    25141 gactccgatg tacgacgcgc ggttcatgtc ggagctggcg aagaaactcg acgacgcacc
    25201 cgaatacgag ccgctgaact cggggcagcc gggctatccg ccgttccttt acggcgagcg
    25261 cccgaacccc gatggcgggg ggccggtgtt caatccactc cacccaacgg tccaaacggc
    25321 tgttctggga tttgtgcgag aaattgccga gcgttatgcg aagtttcccg cattcaccgg
    25381 tatctcgttc aactacttcg cggcacagat gctctggttc gggtctctta gggccggcta
    25441 cgacgactac actatcagcc tgttcgaaaa ggaaacgggg ctcaaggttc ctggagatgc
    25501 ggcttcaccg gaccgtttct ttcagcgcca cctgtacctg acgacgcggt gccggaccgc
    25561 atggatcgag tggagatgcg tgaaaattcg ggatctcatg cggcaaatcc gggatacact
    25621 tcgggaggtg cgccccgatt tgacggttac gctcactctg tggcaggaaa ctgcggtgcc
    25681 cttcctgttt tcgggaatgc acgcgggaac acaactcggc gcgcggcccg gcgcgtacga
    25741 gttttaccgg gagggcggcg tagactggga catgttccgc cacgaacccg gcatactgct
    25801 tgacctcgaa atgggcgatt cgcgggaccg cggagggcac ggcgccaacc cggcagcggg
    25861 agtaaatacg ccgctggaag tggattgcat gttccgggac cacgcctacc tcgacacgtg
    25921 ttcccttgag gccgctggca ggcaggatct gcccggggcg ttccttttca actgctgggt
    25981 tgaggcgtgg ggccgctacc tctggtttcc cgtggagccc gacgactcga atcttacggt
    26041 tgtatcagat atggacggtc agcggcctga tgcgattgtc cgatcccatt cggagtaccc
    26101 ggcggatggt ttctggtggg agccgcagat gcggatcacg ccggcgctca aaggcggcgg
    26161 ccatttcctc gagggcttct gccacgcgct tgcggagctg gacgcgtgcc gcatcacaca
    26221 gggcgggttg ttcctcgata cggcgcacac agaagagctg cgcaggtttg cctcggtgtt
    26281 ccggtccctt ccgaacgaga agttcgagac agtgggacat acgaccgatc ctgtcgcagt
    26341 gcgcagcctt gtcaaggacg accggcggtt cgtgtacttc gtgaatcgcg aatactaccc
    26401 cgtggccgtg accgtggcgt tcaatcaggc gccggggaac gtgatcaatg cggctaccgg
    26461 ggagtccgag ccggggttat cgccctggat ggttgtcctt tctccgtatg aacttcgggc
    26521 aatcatcgta gcgccggaaa tcgcgctgac cgggttcgag gcccggccgc cggcggaaat
    26581 cgcgtcagtg ctcgagcagc gcgcccgcga ggcactggcg gacatagagt ccgtgaggct
    26641 ttcgggggtc ctggttccgg gtatggacga gatggaagag ggcatatcga gagcgatgga
    26701 ggaaaagcgc tacgcgtggc ttcgccgagc gttggacggg tacatcgtgc ggaagtgccg
    26761 ggcgctggcc gccgacagga gcgagtgaag ggtgaggccc gggaatgcag ggtccggctc
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgacttcca tcgcaacttc
    26941 ggaccatttg aagagccgtt gcggctggta gcgaaccgtg ctgatgtcct taagtatgta
    27001 ttcaagcgga catccgtatc gttcgcatat gtcccgcgtt ttctggaggt cgctcctcac
    27061 cgcctccgcg tcaaaggaat cgtgcgcgag gaatgccggg ctgggttttc gtgaatacac
    27121 gtaatcgccg gctatttccg cggcaccacg ttcctcgttc acccacgggc tcatggagac
    27181 cttgcggacg ttcggaatca ttcggacttc attcattttc ccgtcgagcg ggtcacagca
    27241 gccgtagtac acgaggccga atcgcgcgca caggcgactg acataatcca cctcgaattc
    27301 cttgaagatt ttcggggaga cggtggagaa catctgcgcg agtcccatcg cccacatgtc
    27361 cttggtgcgg ggcttttccg ggttataacc gggggccggg agctcatcgg tgaacgcgcc
    27421 cgtgcagtga acccagttct gcggaccgca caggagaccc tccgcttctg cctggtcgaa
    27481 catcgccata tagccatctg tcattcgccc gaccaacctg tggacgtaat ccgggcgatc
    27541 tacgatcgcg aggagggcgc tttccacgct catccaggtg ctgattgggt cccacaggga
    27601 aagatacgcg tccatgcccc agaggcggac gtcaatgatg ccgtcgaaca gatcatgcgc
    27661 gaactcaagc cggcgcgcag ttgcggcttt gtcgtgcgtc agccgcggta tctgaatttt
    27721 ctgcaggtct tcttccgtct caaactggtt tgtgtacttg tgacctacga cgccacttgt
    27781 ggggtctgtg acggcaacac gctcctgaac acggacgccg aacccggtgt tatggatggc
    27841 cttggagacg tggatgtacg ggtcgaccac catgtccccg gggaagtgcc gccactggaa
    27901 cagctcgcga cgcagacggt cctcgtaccc gcgcaactcg gggtcggcgc accggagcgt
    27961 gagttcgtcg ttcacattca tctcattcca gcacacctgg tctatcgcta ccatggggcg
    28021 atccggcttg agcccgttca atttgcgcca cagtgcacgc ttttcctcct gcacgggcag
    28081 ggcggcgatt tcggcctttt gtcgggccaa ttcgcgaatg atcgcgatat cctgtttggt
    28141 cggcatgggg tgagtccttc gcatacgtga cgggtcaggg atgggacata caggacacaa
    28201 gatagggcgc aacaaggcaa gggcaaagat gggaggattg acagccggga ttcccttcga
    28261 gcgcggaggc cggtaaccct ggaagaaacg agatgagcgt tcctacacgt attcgtgtgc
    28321 aagaatctgg aatacgcttg ccgtccaggt gtaggccgga tcgcacaggc cttttccgtt
    28381 gtgcgggtgg aagttctccg ccataccttc cgcaagcacc atgtcgcaaa accgtttgct
    28441 gatctggcga gcggcgccgc gttcgccgag cgccaccagg ccatctacga tgaggagggt
    28501 tgcgggcgcc cagattggtc ctcgccagta ccctttcgga tcaaacttgg cgcttgccgg
    28561 gctttcggtg gccagaccgt gctccgtgag gaatcgtcca tccctgagca cgccggccac
    28621 aagggctttg cgctgttcct ttggaagacg cttaccgaga acgatcggaa cgaaggggag
    28681 cagacaatcc ccttccgcaa catcgtgctg cccggaccgt ggagcgacaa actggccgtc
    28741 cctccatgaa tgggagatca ttttcgccag gagcgtatct gcccgttttt tccagcgtcg
    28801 ggcatctcgt cctctgccca gcgcatcggc gatttccgaa aggacgtcca tctgaatcac
    28861 caggtacgcc gaaaggtcgg gggattcgac gggaaggccc acgtcgaaaa ccgtggcgtt
    28921 atcccagccc gagtcatttc cgtggttgta ctgcggaaca ccgtctccat cgtcgtcgcg
    28981 cgcggtcatc caccagttcg tccacgcggc gaggggagcg tatacctgtt tcagttcccg
    29041 cctcgtgatg aaatccgacc gttccatcat ccaccgaagc gcccacccat gaacgggagg
    29101 cttggtgaaa ttccacacaa tcgagacgtc gttcaggctg tcaggaagcg cgccgttctg
    29161 gtcctgatgg tcgaaaaaca gcatgaattg gtcccaggcc agttctggat tccggtatgc
    29221 aagcgccatg gcattgaagc aggcatccca gcaccagatg ttcgacatcc agttcttgga
    29281 catgagcatc gcgggacggt tcagtctgcc tgacggggct acgaccgatg accatgtgag
    29341 gtactgcgcg agttccgccg cgttctcatg aaccattgag accttgggag aacgcttcgc
    29401 ccatttccgg aactccgctg caacggattt cgtgcactca tcgaacgtgg ggcagggccg
    29461 attgcgcagg ggacgcgtcg tcttgaattc ttccaccacg caatcaagga caccatcgcc
    29521 tgaatcaggc aggcagtcga taaccatgtg agtgcaccgc gggttttgcc agggcgcgtc
    29581 tgcgttgagc gatccggtag caaccgagat ggccagccgg attccgcttg accaatcatt
    29641 cacctctgcg gttgcggaat cgacaaggaa cacgttatcg aacgccgcaa taggcgcgtg
    29701 tgtgaaacga acccccgcgc cgttcccctt gattcgaatc gccgtctgtt cggctatgca
    29761 gaaggataca ctcgccgggc ctgcttcgag agtgagaatc gtggtgttcg cgttgatcgt
    29821 gaaaggagcg ggtatcccgt caaccagcag ttcgacgcgg agaagatcat gcgtcggatt
    29881 tccgccacga acggttcgca ggtaaaggcc cggttggcgg gtcttgggcc ggtcaagata
    29941 cgagaagacg aaataggagc cgtaccggct aaatggcgta cgcgcaagat ccaagtccat
    30001 ggccgtcctc tggttgtgtt ccagcagcgt acttcgcccg cgaatggagg ctacgaaggc
    30061 atatccctca atgtaccgaa ttttgtcacg tcaatgttac gattttgtga ggaacggctc
    30121 ctgatcagtt ccgccgataa agaaagcgcc cgtccgaatc cggacgggcg cgagagatcg
    30181 acggatgatg aacctatggt caggcgaact ggggcagcca gggcttctcc gcttcgaaca
    30241 tttcgtcaac catggaccgg atttgccgca gatccagcac tgccatggtg agcgggtcgt
    30301 aacagcacgc gtggaaggcc gcgtcacggt cgcctttgag cgccgcctgg accgcaagtt
    30361 cgatcacgtt gatgttcatg ctgttcaatg cggcgagttg ggggggcaga gcgccgacat
    30421 gacagggttt gaccccgttt ttgtccgcga aacacggtac ttcgaccatt acgtcctgcg
    30481 ggaaattcgt gataagaccc ttgttcgcca cgttgccgtt gaaacgaaac gggacatggg
    30541 tctggattcc gttgatgatg ttggaaccgt attcgtgcga acggcggaac acgatcgggc
    30601 tggtgtcaga tgcgaatcgt tcctgatcgg cctcccagcc ttcccggtcg ctgccgtaga
    30661 gctgtttgat gaaaccggag ccgccattcc agctccctcc ggggcagtat ttcgccactg
    30721 actccggtgt tttgcggaac cacgcgttgt attcgctgtt gtgcccgctc ccttcggtga
    30781 cgaaatagcc cagatgctcc agcatttcgc aacttgcggt aaggctgttc cagtgttcgg
    30841 gtttgcgagc agcttccttg attttcgggt aaaggtcctc gccgttgtgt cgaatccgga
    30901 gaacccacgc ttgatggttg ataccggcca cccagtagtc gatttcgtcc cggggcacac
    30961 ccgcccagtc ggcaagcatc cccgtggttc cctgcacact gtggcaaagc ccgacgaggt
    31021 tgattccggt ttcgatgtcc atcgcacggc agagcatgct catcggattc gtgtagttca
    31081 gcacccacgc attcgggcaa aggtccgcaa tgtcgcgggc aattcctact ttcacgggaa
    31141 ttgtccggag cgcccggaac acgccgccgg gaccgtggct gtcgccaatg cactgatcca
    31201 cgccgtattt catcgggata tcgatgtcaa atgcgattgg ctcatatccc ccgacgagaa
    31261 tcgccatgat gacgaaatcg ctccctttaa gtacttcgcg gcgatcaagg gtagcgacca
    31321 gtttgcccgt gctccctgcg tcgcgcatta tgcgttcgcc aatgcgccgc gcgtaatcga
    31381 ggcgcttggg atcaatatcc acgagcgccc agtcgctgtt gcgcaacgct tcgaagctga
    31441 ggcagtcaat catgagggtt ttgaaaaaac caaggcttcc ggcaccgatg aaggtgatct
    31501 tgggcatctg gattcctccg ggggaagggg agagttcgcg gcgtaacggg tacgaaaagt
    31561 cacaaatcat gaagaaacac ttgaggttca ggtaatacgg tagccactac caggaacggc
    31621 gtcaagccta attcgggcag tttgttggcg cgggagccat gccggggcga aatcggagcg
    31681 atggggtagg gaattccgcg accggcggcg gggaggtgag ggttgaaccg cggtatcctc
    31741 catggtaccc tatgtaatga tgattgactt ccacaacttc ctcgtgcccc cggggccatt
    31801 tttgagccgt ttctcgatgg gattaccctt cggcggtgcc atcgggccgc acttccttcc
    31861 aacaggatga ctcgaatgaa cgtactcgtg atcaactgcg gcagttccac aatcaaatac
    31921 cagcttatca acaccgatga tcaacaggct cttgcggcgg gttcggtcga acgtatcggg
    31981 atggcggacg cggtgttgtc gcatcagcct caggggcggg aaaaggtccg ggtgacgggt
    32041 gagattcttg atcacattca ggctattgag tacacgctgg cgattcttct gagcccgcag
    32101 tacggggtga taagggacac ctcggagatc gacgccgtcg gccaccgcgt ggtgcacgga
    32161 ggggaaaagt tttccggctc ggtgattctt acggacgagg tcatggccca gatccgagag
    32221 tgtattgagc tggctcccct gcataaccct cacaacgtgc gcggcatcga agcctgccag
    32281 cgcttgatgc cgaaggtgcc ggaagtcgcg gtgtttgaca ctgcgttcca ccagacgatg
    32341 cccgactacg cgtacatgta cgcattgccc tggaagctct acgaacggca tgcgatccgg
    32401 cggtacgggt tccacggcac atcgcacggg tgggtggcag cccggactgc ggaattgctc
    32461 ggaaggccga tttcctcgtt gaaaatggta acgtgccatc tggggaacgg gtgctccatc
    32521 gcggcggttg acggcggtaa atcggtggat acctccatgg gatttacgcc gctcgaaggg
    32581 ttagtcatgg gcactcggtc gggggacatc gaccccgcga tcattctgca tgtgatcggc
    32641 aaggaagacc tcaatgttca cgaagcaacg gcgctcctga acaagcacag cggcatgctg
    32701 gggctttccg gagtatcggg agatatgcgt gaaatcgagg cccaggttga ggcgggagac
    32761 cccaaggcaa cgcttgctct gcgagtgtac tgctatcgca ttcgaaagta tatcgccgcg
    32821 tacgcgggcg ccatgggcgg cctggacacg attgtcttca ctgcgggtgt gggagagaac
    32881 tcgtctctgg tccggaagct ctcctgcgaa gggctgacgt tcatgggcgt cgaactggac
    32941 gacacgctta acggcaccag aagcaaaacc gggcgcgtta tttccacgga caccagcaga
    33001 gtgaaagtcg ttgtcatgcc gaccaatgag gaactggcga ttgccttgga aacggcccgt
    33061 ctcgttgcgc ctctgaggca atgacctcag ttccgcaaga atggcgaacc ggtcactgag
    33121 ttcctgccac gcttggctcc ggacggcact gcgatggggc gcgaacggcg acaacgcgag
    33181 ccgggggaaa gccttgaccc cgcgctgaaa aacgcgctca cgtcgagttt ccctggcgtg
    33241 gcgcggttgc aggctttttc ggcgtcgcag tcggatgtgt gttgaccgcc tactccagcc
    33301 tgtacctcga ttcctctctt ctcggcgatg actacctgcc tatcggagcg ttctcccttt
    33361 tcctcggatt actcgcgatc aacgcgtccc tccggcggac aggttggggg ctgacgaggt
    33421 cggacgttca taccgcgtac atcatgatgc tggtttccgc agccgtgccg gtgaatggtt
    33481 ttgcggcacg tgtcctgccg atgataacag ggctttacta ttacgctact cccgcaaacg
    33541 gctgggagga gtatttcttc cgccacgtgc cgaattggat agggccgcgc gaccgcgacg
    33601 tggcgcgcac cttctacgag ggaacgatca ccggcaacgt gccctgggaa tcgtggttga
    33661 agccgctggc cgtctggttc atcccttttg ccggagtggc gctcatggtt ctggcgctgg
    33721 gtatctttct gcggcgtcag tggatagaga acgagcgcct cgtattccca ctggcccagg
    33781 tggcggtggc ggtaaccggt cccgggaaca cgcccacgat tgaaacctcc ctgatacgga
    33841 ataagctgtt ctgggttgcc gtactggtgc ctttcgccat aaatgctctc aatggtctcc
    33901 accacctcta tcccgtcatt cccaacattg cggcgatgga tccagacctg aaaacgtccc
    33961 ttggcggtta tctcaccgac ccaattcaac agtacatgtg ccgggacttc tatttcgagt
    34021 tccattttgc cctcatcggg gtcgccatga tgatgaggca ggaagtatcg ctgagcctct
    34081 ggttttttca ggtgttcttc gtcggcgtca tcttcgtgtt catggtcact ggactgggac
    34141 cggggcagta tatgtatacc ccgaaggagc aattcggcta cgtgatgttt gcccgatgga
    34201 cgaaaatcgg agctgcggtt gttgttgtgg cggtgatcct ctggtcactt cgccatgagt
    34261 ttgttcgtgc gtggcgggcg gcctttggcc gcaggccagt ttcagaggaa gggcaggcgc
    34321 cactcgggtt cgccttctgg atgtttgttg caggacttgc cgcgtacgca gggtggacga
    34381 gggcgctcgg gttgccacca ggagccgcac tcgccatgct tgcaatcaca cttggcgcgt
    34441 acatcgtggt cgcccggatt gtgtccgagg gaggccttat ctggtgcagt gtcgcgatgg
    34501 acccggtcct tatctacccg acgctcgtcg ggacgtccgg gctcgacggg cagacgatca
    34561 ccgcgatggc gtatacggga ttcgtgccgc tcgccgcacg cgcaaatgtg cttccgagcg
    34621 tgatggactc gttcaagatc gcgcaggggt ctcgaattcg ccccagccat gtaatcatcg
    34681 tgtctttggc gagtctggcg ctcgcattcg tcgtgagtct tgttgtcgtg ctggctatga
    34741 cgtatgccca gggcgggaac tacatgcccc agaagcacta ctcatacggc ccgaggtgga
    34801 tattcggcaa ggcggcaagc tttttcaccg atgtgacggg tccttcgtgg caggcgatcg
    34861 ccacaatgat cgcaggcatt gccatcatgg cagggatgta caagctccat cgccgttatc
    34921 tctggtggcc gttttacccg ttaggttttg tgatcgccga aacggaagtc atgcagcacc
    34981 agtggatgtc aatcgcgatc gggtgggcaa tcaggtcgct cgtgatacga ttcggaggcg
    35041 ccgccggcta tcggtccctc cgtcccgcgg cgatcggcgt cataatcggg gaactgacca
    35101 gtatcatgtt ctggttcggt gtttttgcgc tcacgggaac cacagggaag tcgttgacgc
    35161 acgcgacctc aacctggtga tcgcgcccgc ggcggacgct gaagacggtc ttccaccact
    35221 gtcaggcgcc gtcggctttg cgcggtgttc gctgaatcgt actttcaaat cgctttcttt
    35281 ggtggcatac tcgccgctga agcactcgtt ggccagcttc gcagtcgctc ccctcatgtt
    35341 accggatgca cgatggccga ggaaaacgcc ggcaaccagc cagagatgaa cgtcgggctt
    35401 tccgcaattc gcgaaaggca ccgcggccat tcagtggagg accgaaccgt tcctccgcag
    35461 accggttctc catggcgggg gctgactctg ggcatcgtcg gggtggtcat tctgagcatc
    35521 gtgacaccgt atgctgacgc gtacatggag tcctccagcc tgtcggcaac gtacctcccc
    35581 accggcgtcg tgcttctttt cctgctcata ctgggcatga ataccctcct ccggtacacg
    35641 cgtttcccgt tcacgcagcg tgagttgatg cttgcctacg taaccatgct ggtgccttcg
    35701 gcgatgccgg cggatgggtg gtcactgcgg ctcgtgccgc ttctgatcga gctggattat
    35761 tacgcgactc cggtgaacga atggaaaatt ctccacggga gcaacattcc gcactggatg
    35821 acaccgcacg ggaatgaggt tgtcacgtgg tttttcacgg gacgaccgcg cggagcgcga
    35881 atcccgtggg agccctgggt gcgcccgttg gcgatatggt gcgtactggc ggcgggtttg
    35941 tacctcatca tgctctcgtt ctcaatcgcg tttcgcaagc gatggatgga cgcagagcgg
    36001 cttcagttcc ctcttgcgca ggttcccctg acaatcatgg gagacgatcc ggcaccgtcg
    36061 tgggtttctt cgtttttccg gcagccgttg ctgtgggcgg gaatggccgt acccttcttc
    36121 ttccacacga tcaacggtct gaacctgtat ttccccgcga taccgagcat tcggctgacg
    36181 gacatcttct tctccaccat ttttgccggc tcgcaattca tcactgagcc tccgtttgcc
    36241 gaatgggcag gcgttcggct gaatttctac tggtcgatca tcggaatcag ctacctgttg
    36301 cgctcagagg tatcgctcag cgtctgggcg tttgaatggt tctacaactt cgaaatgatc
    36361 gtgttccagt ggagcgggta cgggaacggt cagtttgagt ggtctccttt gcacaccttc
    36421 gggtactcgt tgatggcgcg gtaccaacgt gcgggagcaa tcgccgcggc atcgatcgcg
    36481 tattttgtcg catcccggaa agaaatcggg gagatggtgc gtgccgcatt cgggatgaga
    36541 actacgagat accgcggagg cgaagcggcg attccctggt ggagcttctg ggcgttcgtc
    36601 gtcgggatga cgatttactt cacgtggacg gccgcaacgg ggatgaaaac aatcgcggcg
    36661 gtggtcctcc ttgcgttttt tcttggcatc gcaataacca ccgcgcgcat tgttgcggca
    36721 agcggcctcc tctgggtata cgactttttt gttccgatgc acggtctcac ggttcttgtg
    36781 ggtaccgcca gaatcgaccc gaagagcttc accaacgtcg gatatgtgga tttcgtggca
    36841 ttgaataacc gcgcagtcat catgccccag atactcgacg gaatgaagat ctcgcgacaa
    36901 acgggaattc ggcagagcca tttcttcctc ggtatcgtac ttggcaccgt tgtcgccacg
    36961 gtcgtttcga tggcgaccgt gctctggctc gggtacacct acggcggcgc gaacatgaca
    37021 gacttcatgt tccggggagg cggcgagtgg ctgttcaacc gtgtcgcggg atttcagcgg
    37081 taccgcgtct atacgaactg gacagtgatc gggtgcatga gcgcaggggc ggcgttcatg
    37141 atgtcgttgc tttacctcca tcgcacctat ctctggtggc cgctttaccc gctcggtttc
    37201 ataatcgggg gatcggtggc atccgggcag atgtggttcc cggtgatgct cggctggctc
    37261 gcaaaggcga tcatcgtccg ttttgcgggc gcgggagcgt atgaccgttt caaagcaact
    37321 gcactcgggc tcgtactggg ggaatttctt tgtgtgggaa tgtggatggc gatagacgcc
    37381 gccacggggt tgactcttca ccgcgtgttt cctgtctgga caccgcagta ggacacgtga
    37441 actggctgga tgagcatggc acaacacgaa aatgagcctt cagaacctgt aaagccggca
    37501 gaacacgggc cggacctcga ggccgttcgc gagcgccacg aggcgcgctc caatatgcag
    37561 tcggctcttg cgcaagagaa tcagacgatt cacaccggag ctccctggcg gggattgacc
    37621 ctcgggcttt tcggtgtggt gatcctgagt gtcgttactc cttatgctga cgcattcctc
    37681 cagtcgtcgg gcctcgcggc gacctacctg cccaccggtg tgttccttct tttcctcttt
    37741 gcgctcggga tcaacacggc gctgagattc acccggatgg cattcaccca gcaggaactg
    37801 atgctcgcat acgtgacgat gctcgtgcct tcggccatcc cttccgatgg actggccacg
    37861 cgtctcgttc cccttctggt ggagctgtat tactacgcaa ctccaataaa cgaatgggcg
    37921 atattgcatc agccgaacgt tccgcactgg atgacgccgc aggggcagga ggtaatcgac
    37981 tggttcttca ccggcaaacc gaccggtgcg gcaattccat gggaagcatg ggtgaagccg
    38041 ctggcgctct ggatactgct tgcggccgcg ctgtgtctca tcatgatctc cctcagtgtc
    38101 gcgttccgaa aacgatggat ggatgccgaa cgcctgcagt tcccactggc gcagatacca
    38161 ctgatggtta tgggagatga tccgaccccg tcatggtatt cgtcgttctt tcgccaaccg
    38221 ctggtgtggg caggcattgc aattccgttc ttctttcaca ccgtgaacgg gttgaacatc
    38281 tatttcccat ccatccctcc aattggcctc gcgaacatca cactcgggag tcttttcagc
    38341 ggttcgcggg tcttcacgga cgttcccttt taccgctggg cgaacataca attcaatttt
    38401 tactggtcga tagtcggaat cagctacctt ctccggtcgg aagtgtcgtt gagcgtctgg
    38461 gttttcgagt ggttttacta tatcgagcac atcattttcg aatccaccag catcggtgac
    38521 ggccagttcc agtggacacc cctccacacg ttcggatata cccttgcggc ccggtaccat
    38581 cgcctcgggg caatggttct ggcgatgctg gtgtatttca tcgcgtcgcg cggggaaatt
    38641 cgcgaggtgc ttctggcggc gttcggtcaa c
//
LOCUS       DAOSOO010000008        38107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1114_length_38107_cov_5.660596, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000008 DAOSOO010000000
VERSION     DAOSOO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 38107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38107
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_38107_cov_5.660596"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLT86_01215"
     CDS             complement(<1..461)
                     /locus_tag="PLT86_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35267.1"
                     /translation="MNWRKGVLFAGVLGMVAVPAVFANGLQAQFHLGRFWTNPENEPA
                     ESSLMCYPGGVPRNEWSDGSGQQLKRSWLTHTRKHGGSMLTIDWTNELGTLYADAYSY
                     MYRGNDTSSPPRYRLDKPDAFNYLYAVSIQEYMRFPRPKLILVVKSPTSATG"
     gene            complement(607..4122)
                     /locus_tag="PLT86_01220"
     CDS             complement(607..4122)
                     /locus_tag="PLT86_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT35268.1"
                     /translation="MVHAIRRLADVSAVFSLMLMGFLAVPGVRAGTTGKIAGRVTDQN
                     GAGLPGVAVTLEGTRLGATTDPDGRYMILQVPPGVHNVTANLIGYRRSSITSVSVSAD
                     RTTDVNFRLTEATIEVGAIVVRAERPPIEVDVTSSQTVVNAARISEAPVSRMLDFLNY
                     EPGVSVTRGTELEIRGGGPSEIRFQVDGLDRTDGLTSKPYTQLNQVLVSEVTLLTGGF
                     NAEYGNVRSGMVNVVNKDGTERRSAAVALPWVSGVYNMAPARKKHFGPGGYDSDQYDY
                     WLMSSDSPFADSALAGPLYWPLLYEETRTAPEFTDPTNPKYVNPRTSAFRVFDGWKQR
                     ATVVNAARGWFGKRDWTPAEVREAWEWEANMNEQVWKYSNNPDYNLDLAAGWALPKRM
                     GGVVVGYSRTYEMTVVPALRPYNEDQTFEAKFTLTPLDKIKIQFSYMTGRNEGTAAGS
                     TGGAASNPELAASGASVTGNDPVMLRASGDLLNGLTGTSDNTKFNLSYNSLLNGEYSQ
                     YGGTFTYTFSPKTFLSASLGRSSSKWEVNRDLPRADFSDFAPGGRYQPPNTWSGQGWM
                     GFAFGWTDTNGVGGPDKPSSLADALNPNRLAPVSPFGIPYLYYQVPSEAKFIYREIGG
                     DTTTVGRVVSPQGYVMSAYKDLSGVYGIGGGGHVRLTGRSNQIVFRSDFTHAMGSHTL
                     KTGVEFIRGDLEYHMIQNSALTTNTEWRDYGGNYPAVNPSILGVYLQDKFETNGMIAN
                     GGIRIERFDAGQNAYFYNDMFDNDLFVNHGTRYFKDLSVALGWDTLAYGPVPVNNVSD
                     YFKVKEKLGVAPEPWDVVRIFPQSDNIVHWRIAPRFGISHPVSYRTKFFFNYGIFYSM
                     QKAAVMYGHSDHDGRAAFNGWVADMFNPNLRPAKTTMYEVGVEHVFPLGLVMTVRGYA
                     KYNVDQVTRISVTNTLRSDFRGYSIFRNANYEDIQGAEIKISRSSGRFVNGWLTYQKT
                     SSRSGMVGLTVVDLNTVNIQPFESFASTSDPKGYFQGMLRVGTPMDWGNIRGGWAVSV
                     VQSYASGGEVIYNPEALPRREIPDENILPSASSYNTDLKLSKAFQLGRGRSLSAYLDI
                     GNVFNIKRLNGGGIQNYIDYLGYIYGQRLQGNDMKVGDESTFAMFTRPWKDKDGNWKR
                     PTSAQTEWMHHLYPRSYRVGVRFDL"
     gene            4733..5413
                     /locus_tag="PLT86_01225"
     CDS             4733..5413
                     /locus_tag="PLT86_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35269.1"
                     /translation="MVRVLGGLLAVLSAVLSARASDWAPGAGINVVTAHVVRGDKRNG
                     LSLQPAFWFNAFQERGLFTSWASVAPADPALDDLVLDFRAGVPTAGWGWMGGVVHWDW
                     SRPPSDSLRSHSLEIGPTLTWFKPPYRPSLTVTYDFAQRNGVLFTVNAPYTVRMTWLP
                     ATTFTPEVAFRFAGAGNRVSAEYFSFSALLERKIRGVTLLPTAGIVPRSELSKSLVWA
                     GIHVNVVR"
     gene            5400..6155
                     /locus_tag="PLT86_01230"
     CDS             5400..6155
                     /locus_tag="PLT86_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35270.1"
                     /translation="MWLDRIAPFAFVFLGAVFFGCAPTCVKTPPPPAGELIAEVLSVP
                     GAHAIQAYGTLRVTSPEGNYSGSAVVFYSYPESVKVVLQGGFGTTFAEIALAGGKGIA
                     YLPQQRQALELADGSGLLFGSVVVYPSMLLHLLKPVDAERLGQDAIISTECSQYVLAS
                     RTAAGTRIWRLSARARRLEAEEFRSVENTMFWTRTFGAANGIRVPKSFSVRLGETTMS
                     AEFTRINVSPVMRPNTFAVALPPGIEVMTVPRE"
     gene            6244..6762
                     /locus_tag="PLT86_01235"
     CDS             6244..6762
                     /locus_tag="PLT86_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35271.1"
                     /translation="MLPNVPSDPGALHPLVVHFPVALLLVAPLFLLMGAISKNDRPQL
                     RSCVLVLMALGVLAAWVAVWTGEHAATIARITAPQAASVIEQHEESAEATAVIFTVLL
                     AVFAIRVAVDYLRKENTVRRPMRVFCIVFLVVYCVCSLVVVDAAHQGGRLVHQFGVRA
                     QMGSAPPGGTNP"
     gene            6828..7403
                     /locus_tag="PLT86_01240"
     CDS             6828..7403
                     /locus_tag="PLT86_01240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT35272.1"
                     /translation="MGHSKAMVLSHDAEWRWHVRADMPPPRVGEVTHLLKPHGECVLT
                     ILTSQQSVWLHTKDFYPAGCYRLPTGGIKPEEHPDGAARRELREEVGCAGSCRILRLG
                     KITYVEASGTAYPFVSYLYQVAPVEKTPTSADESERISGWKTVPISHLGAVASELRAL
                     PAEWVDWGCFRAVAHDAVREILRREAQSPCL"
     gene            7410..8063
                     /locus_tag="PLT86_01245"
     CDS             7410..8063
                     /locus_tag="PLT86_01245"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT35273.1"
                     /translation="MIQNELLQTLKTEAYTAVVADVLDSLGLQNQILPSRIRPLGNSE
                     AVVGIARTILVRDVVSASPHPYEVEFALVDDLREGDVIVAQCGEREAAFWGELLSTAA
                     AKRGAVGAVVDGFCRDLRQISELRFPVYARGMSPADSKGRCEAVARDIPIEIGGVAIH
                     PGDMIFADLDGVVVVPAGSIDETARGALEKVRGERTVFNALQSGMSTREAYDKWGIL"
     gene            8086..8481
                     /locus_tag="PLT86_01250"
     CDS             8086..8481
                     /locus_tag="PLT86_01250"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOT35274.1"
                     /translation="MAEKDAKLTQEQQDTFAFMAMVELYQQTAWMALGKIADPATKET
                     KANLPQARWAIDTLAMIERKTRGNLSDQELRFLREILHMLRMNYVEEANKPVGEPPST
                     QDKTETKPEKETATHDEPETPPPAEPQNE"
     gene            8478..9572
                     /locus_tag="PLT86_01255"
     CDS             8478..9572
                     /locus_tag="PLT86_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOT35275.1"
                     /translation="MKLGPLLFEPIYRSYVWGNRNFERLFGRVLPPEVPTAESWELSD
                     VDGAISRVESGPFAGRSLRELCQEFAADMFGEQSGSSICGGRGFPLLVKFLDAERVLS
                     VQVHPGDQFAAEHENGSIGKTEMWFIVEARGNAEVIAGLRPGTTRAEFLEAAQSGSLE
                     PLLQHHCVRAGDVVLVPAGRVHAMGAGIVALEIQQTSDITYRLHDWGRVGPDGKTRPL
                     HLDRAIEVIDFSDEGRAVTQPVQRTEDWGVWSLLGVTLFFLTESFSQFRSVPCTTRSG
                     SPDILVAMNGSLRISWDHGDVIVPPGRTALVPSCVGLYVLEATEDTLVVRSRVPDRSD
                     VLGDWTPRSNEERTAVLDVCDTRVAELVTA"
     gene            9875..11272
                     /locus_tag="PLT86_01260"
     CDS             9875..11272
                     /locus_tag="PLT86_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOT35276.1"
                     /translation="MFSLAGSAGGVVVLAFVGAAFFSRRHGRKGSGSSGTPRIRTVSP
                     GPSGSAPEPASARTVIAAVAKPFQSLAAGVVSLKREVWKENIPRLAGMVFLLLAVGAL
                     VVGLVEVLFGAENQRANFGSPINALYWAVVTLATVGYGDLAPQTTAGRLLTSVFILVG
                     MVTISVFTATLASVFTAKRIKEGRGLEKVKATNHVVVCGTGRHLDTIIRYLTRAGITR
                     KQPIVLVNSLTEEAMNEILYRYSHLDIQHVHGDFTQESVLRRANIEDAVAAIIQAGDD
                     TQDRSRADNRTLQAALAIKALNHDIKLTAEALDPENEAHLRRANVDDVIVSGEFSGYL
                     LGACTVSPGLDVALRELLTVEVRNDMIRNPIPESFRGKTSKELSEWFRRREAMLIGII
                     VEQKVLNLDEMLQDDFSSIDRFIRSTFSAAGKELVAQGKGRTEVVVNPPDDRVIGPDD
                     AAIIISPRVIPVTET"
     gene            11304..11807
                     /locus_tag="PLT86_01265"
     CDS             11304..11807
                     /locus_tag="PLT86_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT35277.1"
                     /translation="MPGYETLKTASLLKGLTDSQLKKLWDAGESKTIKAGDTIIREGE
                     VGDTMYVLFEGTVEVSQVLVLRLGNAAIAEKDKTLIRLEGTARPCFGEMSLLEDAERS
                     ATITALTDCQVFVIDRSSFNRLVEEDPVMGTKLLRSIAVVLSGRLRKANKDVLKLTTA
                     LSLALTD"
     gene            11882..13117
                     /locus_tag="PLT86_01270"
     CDS             11882..13117
                     /locus_tag="PLT86_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT35278.1"
                     /translation="MRERFGFIASALAAFLFVGCPAPPRYAPTSRLAPRPGELPVRIG
                     LAEATGSVRIEADQGLRLVAGETVRLAPSFALSPSRGHIAVNGARTPHKRVTCFASTP
                     LSCNGVPYRGQLEVSAVEGALRVVNIVGLEEYLAGVVPAEIGRLSTREIEAVKAQAIA
                     ARTYTVANRGRRRAQGFDLYADVRDQVYMGVRGEHTVATRAIEQTRGQIVTYRGEPIQ
                     AFFHSTCGGFTASIEDVWNADPLPYLKGVPDRDSHGFHCETSRHFRWSEEYSPGVLRT
                     ILSENLFREVPNAPRKIGTVRKIEAPERSSSGRVRYLKVETNRGHWRVSKDRIRWALR
                     RPVDGNPILRSTYIRIFSEYDAHGSLQRVVISGAGNGHGVGMCQWGAIGMARKGYPAE
                     QILKHYYRGVTITSMRMAD"
     gene            13144..13887
                     /locus_tag="PLT86_01275"
     CDS             13144..13887
                     /locus_tag="PLT86_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT35279.1"
                     /translation="MRISSLATGCIVAISFVALGCPAPPPATVPAAGDTQNNDTTATT
                     TAPELPDPRGQLPRTATPAPAEATDGPDAEPASELESAVYSVQVMSSSSRDAAEAAAR
                     QVRSLTSQPVEVVQESGGAWRVYAGRSGTRTPMDRLRDQLISAGYSGAWTKQRVVETS
                     VRDNTIPTGESVYSVQIFVSSTPENARRVAAEARSVTRLPVEVVEMDGYWKVFVGRSP
                     TRSAIDAERDALRQRGYPDAWTYRRQGTR"
     gene            13957..14715
                     /locus_tag="PLT86_01280"
     CDS             13957..14715
                     /locus_tag="PLT86_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOT35280.1"
                     /translation="MNFDLNIGRYVAGTSPIHRLDPRTKLGLALFLCIGILQGTSATA
                     TAWHCVLATVLFGISGLKARFVARNLRPFVYLAGMILASHLLINGAGSWQTGISVGVR
                     LLVMVSVVSLFSWTTQPLATVAGLRRLGSPLARLKVPVDAAATSIGLAMRFAPIVVSE
                     AQTVLRAQAARGADFRGVKRKLTLIVPLLGALFDRSFARAEVLAEAMQARGYDPSAVR
                     TSYHRLGFRGVDVIALAYVAIWLGGAVIMERYAP"
     gene            14712..15446
                     /gene="truA"
                     /locus_tag="PLT86_01285"
     CDS             14712..15446
                     /gene="truA"
                     /locus_tag="PLT86_01285"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT35281.1"
                     /translation="MSTRLRLDMEYEGTEFAGWQVQPGLRTVQGELNTALSTILRTPV
                     RVIGAGRTDAGVHARGQVGHADVPDAYPPLARILAGVRALTGPDLLVREITVVPGDFH
                     ARFSARARTYEYRIALVPSPLERRFQWFVPYRLDVGSMSVAAQKLEGRYPATCWCSSS
                     APDTTAIVHVQRTEIERRATEIVFRITSDRFVTHMVRTIVGTLVEIGRGVRSPEDIGA
                     IISSQDRARAGPTAPAHGLCLQSVEY"
     gene            15451..16704
                     /locus_tag="PLT86_01290"
     CDS             15451..16704
                     /locus_tag="PLT86_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT35282.1"
                     /translation="MDYSYGEWEHQDQPRRTRFWTGVLLGIGVSVVALFIANRITGRH
                     WGGRLSVPEPPAAFSLVSGVGDTTGDVGNSRRNAIVRAVEIVAPAVVTISVTQLRRER
                     AVPFADDPMWQFFFPELRQEFVRRVQSMGSGFIVSPDGMILTNEHVIRGATQIVVNLP
                     DGRSFDAKIFGEPDEQADIAVLKIDAQNLPTAPLGRSDDLMIGEWAIAIGNPFGFLIR
                     DAHPTVTVGVISALNRDFSPSSAGGRTYRDMIQSDASINPGNSGGPLVNSEGYVIGIN
                     TFIFTQGGGSEGVGFAIPIQKARQVLDDIVQFGEVRHNFWTGIHIQDVNRWIAESLGL
                     QSDRGAIITNVEPNSPGAKAGLQRGDVIISVNGKAIASDRDVRDEFLGAVVGQRVELR
                     IIRNGRELTVTLVLEEIPRNRRDRG"
     gene            16697..17335
                     /locus_tag="PLT86_01295"
     CDS             16697..17335
                     /locus_tag="PLT86_01295"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOT35283.1"
                     /translation="MAKEGLPFVLGGVCAGILLLAAWHGLRWEPLRILGILALVFGAF
                     SLFFFRDPRRTPPAGEGLVLSPADGKVVEIVQEDDPYVGTNAIRVSIFLSVLDVHVNR
                     NPISGSVTAMVYRPGKYLMAFNEKASADNEQTHIAIKGERGTVAFKQIAGFIARRIVC
                     RLKVGDTVRAGDRCGLIRFGSRVDVILPPDASIRVAVGQRAVGGKTVIGVLP"
     gene            17332..18114
                     /gene="pssA"
                     /locus_tag="PLT86_01300"
     CDS             17332..18114
                     /gene="pssA"
                     /locus_tag="PLT86_01300"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOT35284.1"
                     /translation="MMPLVTQALPSVMTLGSLFCGLLAAIKAMEGTEEAFVTAAWLII
                     AAGLFDGLDGKVSRIFKTQSHFGVELDSIVDVCSFGFAPALLIYQFILLNASGFSGIA
                     FPVAFVFLACGALRLARFNAQLKGFDKAAFKGVPIPAGAGMVASFVVFSRSEFIIDLG
                     IDWVLPFLAFGMALLMVSTVRYDKTPRFSWHGPTSERVRLVVMIAFLVIAVWYPAEAF
                     FSFGVYYVLYGLVRALGNAFVRHPAVAPGQPSLERVKEGSRR"
     gene            18115..18384
                     /locus_tag="PLT86_01305"
     CDS             18115..18384
                     /locus_tag="PLT86_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOT35285.1"
                     /translation="MPRYRSTGVLFASLLIGLLGATVLSAALSLFVGPASVVHRVLIN
                     SFTYDSGVLVLNLIVVTISFGITLNINLLTILGLMSAFYWWKYRL"
     gene            18410..18592
                     /gene="tatA"
                     /locus_tag="PLT86_01310"
     CDS             18410..18592
                     /gene="tatA"
                     /locus_tag="PLT86_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT35286.1"
                     /translation="MIGGWEWVIIIVVVLLVFGARRIPELGKSLGQGIRSFKEELAGG
                     KQRKDEPKDDSSSGQS"
     gene            18650..20098
                     /gene="gatA"
                     /locus_tag="PLT86_01315"
     CDS             18650..20098
                     /gene="gatA"
                     /locus_tag="PLT86_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOT35287.1"
                     /translation="MNNPNFTAAALVPQILEGSVRATDVLDTVLATIREQEPRVHAFL
                     QVTEDLARSQAEQVDRKIAEGKPVGPLAGIPVGIKDNLCVRGGRTTCASKILEPFVAP
                     YDATVISRLRDADAVFVGKTNLDEFAMGSSTENSGFGVTRNPIDLERVPGGSSGGSAA
                     AIAAGMCVLALGSDTGGSIRQPASFCGIVGLKPTYSRVSRFGLVAFASSLDQIGPMAQ
                     TVRDAAMLLNVIAGADAHDSTCSPDPVPDYLEGLEDGVRGLRLGVPREYFGEGLAPEI
                     KTGLKQVIHRMEREGAELVDISLPHAPYAIGTYYLIATSEASANLARYDGVKYGFRAK
                     DVKNLGEMYRRTRSEGFGAEVKRRILLGTYALSSGYYDQFYVKAQKVRTLISQDFASA
                     FAKVDAILTPTTPDTAFKLGEKTSDPLQMYLEDIYTVSANLAGIPGISVPAGRDSSGL
                     PFGMQVLGPQFGEPVVLRVARAVEMLRPGEAG"
     gene            20126..21601
                     /gene="gatB"
                     /locus_tag="PLT86_01320"
     CDS             20126..21601
                     /gene="gatB"
                     /locus_tag="PLT86_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT35288.1"
                     /translation="MSNYRVVIGLEIHLQLATKTKVFCGCSNEFGGEPNTHSCPVCLG
                     MPGVLPVFNRQVLEYALRLACALGSRVSERCRFVRKNYFYPDLPKGYQISQFHECLAY
                     GGAVEIDVSGTTKRICLDRIQIEEDAGKSLHSEDSHMEDTLVDVNRCGTPLLEIISKP
                     TPGSPDCVYDPSAYLNSPDEAIAYWVKLRQLTRYLGISECNMEEGNMRADANISIWDE
                     ARGSFGTKTEIKNLNSFKFAHRALTQEIARHIEILSAGGKVKQETMLYDSSTDTVAPM
                     RSKEEAHDYRYFPEPDLVPVVVTEEFLAQVRKNVPELPDARKKRFVSEFGLNQDAADL
                     LSSERALADYFEGVVKSGAEPRTAANWIMGDVLRETNARKMDVSVFPVSPPRLAELIA
                     LVVSGTINQNGAKDVFAEMVDSGKQATQIVEARGLAQISDRTALAGVVAEVMGRFPGE
                     IQRFRAGEVKLMGFFVGQVMKATKGRANPALVNDLVREYLS"
     gene            21598..22602
                     /locus_tag="PLT86_01325"
     CDS             21598..22602
                     /locus_tag="PLT86_01325"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT35289.1"
                     /translation="MNFRWLAYSGLAFFLVASRVEAFGSGDDSGAAGFKSLFLVATSR
                     SAALGGADCALSANPLAAAGNPSASGGIASRTAGIAVTDYVLDVLPVVAYGAYPARHG
                     VWSVVLGNVSFGEFQRTNETAAEVGTFGANDLSLRVGWAGPLRLGIVGGVSLGWIRST
                     IDAYSASALVMNVGLQWHAPNQQTVVAVSAENLGGALSSYVGGSQGLKDDVPTEFHAG
                     VSHRPAHFPLPLLLVSDFTLPRDNEVSASVGAEIRPFEPLFIRVGYASLVRYRSNTDS
                     DGTHKSGFAFDDRHGSAFGGSGLAAGFGVVHKSLSLDYAYSLAGAFGGVHRVAAAWAW
                     "
     gene            22755..22836
                     /locus_tag="PLT86_01330"
     tRNA            22755..22836
                     /locus_tag="PLT86_01330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22789..22791,aa:Leu,seq:tag)
     gene            22939..24210
                     /gene="tig"
                     /locus_tag="PLT86_01335"
     CDS             22939..24210
                     /gene="tig"
                     /locus_tag="PLT86_01335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT35290.1"
                     /translation="MKLEVTETAPCSRRINIEVEPDAIEERITSTVKEYRRTRTIPGF
                     RPGKATDSVIRRWFGKDIRAQVIRDLMEESWQKALEENKLQPVDQAQIDSVNAEPGEP
                     LRFSGTVEVKPQFDVQDYKGLPAIRPVHTVTEEDVTRQVNFLRERRATETPVERPAQN
                     GDVVVIDLQKLDETGVPLIGQKQDNLRWVLGGTGSVSKDLDEQIVGMAQGDTRNVTFH
                     YREDFYDPNRAGKEEKASITLKEVRERIVPEITEDFLKELGEYATENDLRKDLEADIK
                     RRLDALAKQQVHVGLKSALVERNQIEVPETLVQRLLTRMFQQHKQQGHEHDENEFRAE
                     HREDALREVRASLLLERIAAQESIVVPDEDVDARIARMAASMGTSVRNLRDYMINNGQ
                     YEQIRYELQEEAVLNFLEEQAAIEESPLPKK"
     gene            24277..24879
                     /locus_tag="PLT86_01340"
     CDS             24277..24879
                     /locus_tag="PLT86_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT35291.1"
                     /translation="MNLAPMIIERDGRGERAYDIWSLLLKKRIVFLGTAIDDAVSNLV
                     VAQLLFLQSEDAKTPIEMYINSPGGSVSAGLAIYDTMKFIDAPVHTVCIGLAASMGAI
                     LLAAGEKGHRTALPHARIMIHQPWAGGIQGQATDIAIQAEEILKTRNRLNELISMDTG
                     RSLEDIARDTDRDKFFSAEEAKEYGIIDSVTTKQIAKIPA"
     gene            24915..26168
                     /gene="clpX"
                     /locus_tag="PLT86_01345"
     CDS             24915..26168
                     /gene="clpX"
                     /locus_tag="PLT86_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT35292.1"
                     /translation="MATRKREEELRCSFCNRGADQVKRLITGPNVHICDECIQMCNEI
                     LNDEKRTRPALQDKPLPKPAEIKRHLDEYAIGQERAKRILSVAVYNHYKRIRSAGANP
                     ENVELDKSNILLVGPTGTGKTLLAQTLSRILDVPFCIADATVLTEAGYVGEDVENILV
                     RLLQAADYDVARAEIGIVYLDEIDKVARKSANPSITRDVSGEGVQQALLKVLEGTISS
                     IPPKGGRKHPEQPLIQINTKNILFICGGAFEGLETIIQERIGRKYIGFNAPPESARKK
                     EASSILHHAEPDDLLKFGLIPELIGRLPVVAALDELDDEALMRILLEPKNALVRQYQH
                     LLGMENVRMRFEDDALKFIVDEARRRGSGARGLRAVIESAMVDVMFEIPSLSGISEVV
                     LTVDPAARKLKSLYLPDDGLRKQIA"
     gene            26195..26824
                     /gene="yihA"
                     /locus_tag="PLT86_01350"
     CDS             26195..26824
                     /gene="yihA"
                     /locus_tag="PLT86_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT35293.1"
                     /translation="MRVVSAEFVRSVVDLNELPAGRMPEIAWAGRSNVGKSSLINALT
                     GRKGLARTSNTPGKTREINFYVIDSSWHLVDLPGYGYVAAAESDRVRWQALIEPYLER
                     RKQLCGVAVLVDSKIGPTDLDQTMIEWLTRRGRNFAVVISKADRISDRHAADLAESLR
                     GTLPETAPVLITSAKQKQGLRELEGWILERLSGWRKETPVRATWHSETV"
     gene            26841..27986
                     /locus_tag="PLT86_01355"
     CDS             26841..27986
                     /locus_tag="PLT86_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOT35294.1"
                     /translation="MKTRLYTPGPTMVPEQVSLDMAAPIIHHRTPEFSKVLQSCHEGL
                     QYLFQTKNPVLMLTCSGTGAMEGAVVSVLSRSTLTLVVRGGKFGERWGEICAAYGVPF
                     VPLDIPWGESITAEQLEEALAANPGVRAVCLTQSETSTGALNDIKTLAAVVKKRNALC
                     LVDSITGCGVHELRVDDWGIDVAVTGSQKGLMLPPGLAFAALSDEAWEAVKRTDLPSY
                     YLSFAKHRKSWEKTTTPWTPAITLLTGLARALEMIRSEGLEKLWERHQNMAEAMRQAV
                     RTLGLPLFPRVPANVQTVIALPEGLEYSRLSKQLFNVYGMKTAGGQDQLTGKILRIAH
                     LGYLDDGDIVAGVSFLERALADVGWKVNSGEAVAAAQRVLARTPAAQ"
     gene            27997..29613
                     /gene="serA"
                     /locus_tag="PLT86_01360"
     CDS             27997..29613
                     /gene="serA"
                     /locus_tag="PLT86_01360"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT35295.1"
                     /translation="MTEFLHHKVLISDDVHEICKTRLEAAGIEVDYRPGIKGAELLSA
                     LKDCTGLVVRSATRVTGEIIAAGPKLQVIGRAGVGVDNIDVEEATHHGIAVLNTPEGN
                     TTSAAEHTIGMMMALSRNIPQACAAMKAGKWSRSAFTGTEVRGKVLGVIGLGKIGREV
                     ARRARGLDMNVVCYDPMISPAAAEATGAQLLPLDAVLEQADYLTLHIPSNANTHHLLD
                     ADRLNKCKTGVRIINCARGEIIDTDALLDALNSGKVAGAALDVFENEPPDMTAPIIQH
                     PHTIVTPHLGASTAEAQHLVAEQIGQLVAEALTTGTIRNAINMFPMDRQTYERAKPFL
                     RLAERLGSLQAQVCDGQPVSLTLEYFGDVLEHPTGLLTASVLVGMLKQLESGTVNLVN
                     APVLAQERGIRVDEMRRTGDDDYNNVVTVTYQTRLPSGETLTRRIAGSVFGEDSPRVV
                     RIDGHSCDVIPEGRLLIMWNRDVPGVIGEVATVLGTHNVNIAFGSYGRDVPGGLAVMV
                     YVLDSDIEESTVDTLRKLKNLHTVKYARLA"
     gene            29627..30082
                     /locus_tag="PLT86_01365"
     CDS             29627..30082
                     /locus_tag="PLT86_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOT35296.1"
                     /translation="MKIAFASDHAAFDLKNGVLPYARALGHEVVDFGCATKEPCDHSD
                     FSIPVAEAVARGEFDGAVLACGSGQGMTIGANKVDGIRATLCLTPEAARLARAHNNSN
                     ILVMAGRSLTPAEGQAIFEAWVSTPFEGGRHARRMDKIAEYERQRCAKR"
     gene            30092..30538
                     /gene="nrdR"
                     /locus_tag="PLT86_01370"
     CDS             30092..30538
                     /gene="nrdR"
                     /locus_tag="PLT86_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT35297.1"
                     /translation="MRCPFCGKLEDKVVDSRASRDGATIRRRRECLVCSARFTTYEVV
                     ESVPVTVTKRDGRKEVYDRAKLQRGIELACAKRPVTQEQIERLVDEIELELFHGQTRE
                     VKSSRLGDMVLQRLEQLDKVARLRFASVFKDFETPGAFIDELKLFG"
     gene            30552..30638
                     /locus_tag="PLT86_01375"
     tRNA            30552..30638
                     /locus_tag="PLT86_01375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30586..30588,aa:Ser,seq:tga)
     gene            30758..31720
                     /locus_tag="PLT86_01380"
                     /pseudo
     CDS             30758..31720
                     /locus_tag="PLT86_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            31730..32416
                     /locus_tag="PLT86_01385"
     CDS             31730..32416
                     /locus_tag="PLT86_01385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT35298.1"
                     /translation="MVGTAKPERAFFAHPTAIVDEPCSIGAGTRIWHYSHIMKNAVIG
                     ENCVFGQNCNVDSGVVVGNNVKVQNNTSLYTGLIVEDNVFLGPSCVFTNVTNPRSEIS
                     RRGLYEKTILRRGCTIGANATILCGTTIGRYAFVGAGAVVTKDVPDYGLVVGNPARFR
                     GWISRHGLPLRTPDANGVYVCPESGLRYKEVAPGVIRCLDLDEEAPMPEEMRVGLKSY
                     DAIVHPERSA"
     gene            complement(32424..32903)
                     /gene="purE"
                     /locus_tag="PLT86_01390"
     CDS             complement(32424..32903)
                     /gene="purE"
                     /locus_tag="PLT86_01390"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT35299.1"
                     /translation="MGNEAKETGKTDVLILMGSSSDMPVMEKAAAQLQEFGLSSEIVV
                     SSAHRNPDRTAQIAREAQDRGVKVIIAGAGMAAALPGVVAAHTSLPVIGVPCASGPLN
                     GFDSLLSIVQMPPGIPVACVAINGAKNAGVLAAQIIATGDPDVAVKVAEFRRKQSAA"
     gene            complement(32916..34226)
                     /gene="purD"
                     /locus_tag="PLT86_01395"
     CDS             complement(32916..34226)
                     /gene="purD"
                     /locus_tag="PLT86_01395"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT35300.1"
                     /translation="MFETKKKDVLIVGQGGREHALAWLLKRQGGVNRLMAAPGNAGIA
                     SIAECFDVKPTDIDGQVALAGKEKPDLVLVAPDDPLALGLVDRLQAAGIRAFGPTAAA
                     ARIESNKAFAKRLMWEAGVPTAAFKTFSDPEKACDYVSSVSHRVVVKASGLAAGKGAI
                     VTSSRDEAQEAVWEIMVKRTFGDAGNEVVIEEFMEGEEASLFAICDGRNYRLFVSAQD
                     HKPVFDGDRGPNTGGMGAYAPAPVVTDEVRQHACERIIEPTLKALAAEGCPYHGILYV
                     GLMITREGPKVVEMNSRWGDPETQVVVPLLRSDMMELMEAAIDGKLDTVSVGEDEGAA
                     VVVMLASGGYPGDYTKGFRISGIEKAAEQEGVILFHSGTAWKDGSLVTTGGRVLGVTA
                     VADDIRTAVGRAYRAVDHIHFDGMHYRKDIAHRAIARLASSTLK"
     gene            complement(34312..34917)
                     /locus_tag="PLT86_01400"
     CDS             complement(34312..34917)
                     /locus_tag="PLT86_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35301.1"
                     /translation="MQESTRGWSEQQWEDYFARQDESFFLALRSRTSREQPTNDVQPS
                     SEPAGTMDDLLAAASADTLLEEEDALIRELYAGLGQMPAWRAVVEFCDRTMEFVAPIC
                     ENVIGSPLESVTRILCRECFLVADYVLAGHELGYDEETLCGNIALCLRARRSLDRCIQ
                     CLDRFGPEYRDACLQLRLRGKIAGVFLERRVSDLRARVWWR"
     gene            35115..35777
                     /gene="eda"
                     /locus_tag="PLT86_01405"
     CDS             35115..35777
                     /gene="eda"
                     /locus_tag="PLT86_01405"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOT35302.1"
                     /translation="MEHPLLRRMLQNGIVGVVRTPTPETAARAVEAVVSGGIKVVEIT
                     FTVPNATKVLETVAASTGDDVVLGAGTVTDSEKAKAAIRAGARFIVAPNTDPATIATA
                     HTLGALAIPGALTPTEVVMAMNAGADAVKVFPISVFGPSYLKALRAPLPDVVLMPTGG
                     VEPDNVFDYLAAGAQLLGAGGTLVESKHIATGNYEAIARKARSFVDAYNEAIARLGSN
                     RS"
     gene            35799..36794
                     /locus_tag="PLT86_01410"
     CDS             35799..36794
                     /locus_tag="PLT86_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT35303.1"
                     /translation="MSEVVTFGEGMVRLSPPHFQRLEQTSTLDMAVGGAEFNVATGVT
                     RLGLTASWVSRLPQNALGRVLVNQARQLGVDTSHVVWSREDRMGIYFLEFGSAPRPST
                     VLYDRKNSAMSRIRPGEVDWDSALAGAKLFETSGITPALSPSAAEATAEALRIARQKG
                     LKTVYDPNYRSTLWSIEDARKVQTPLMRDVSCFITGVSMEGETKEAVLGVTGSSPHDL
                     AIRLVETFGFEVVALTLRGTSGLMRNSLTAVAYSNGRSYETKSYDLEVVDRVGGGDAF
                     AAGFIAGYLEDDIQKALDYGTAFAALKHTVPGDINWTTLQETEDLIKGAGLRIRR"
     gene            36826..37623
                     /locus_tag="PLT86_01415"
     CDS             36826..37623
                     /locus_tag="PLT86_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35304.1"
                     /translation="MRLSIVTDELKTPLAEALSILDGWKVRNIELRGLDGGRIPDGDV
                     ENALSRVRAHGCTVTSLSPGVFKCACDEQEIEKHIERFQRTLELCDSFGCGQVIVFSI
                     QGQESRLAAPPALVVEALRETGRIAAQHGVRVAIENEPGYAATGCRALANLINAVGME
                     NIGANWDPGNAFPFDPEIDDAVRVLAGRIFNVHVKDTSVQGDHRRFDSVGKGQINWKK
                     QINDLAASAYEGCLVIETHCEPGIEKTRESLDAVRRLLQDVTPRIGG"
     gene            37631..>38107
                     /locus_tag="PLT86_01420"
     CDS             37631..>38107
                     /locus_tag="PLT86_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT35305.1"
                     /translation="MAQIKTLEDLERMAPACQLASETLEYLKQAVRAGISTLELDALA
                     EKYIADRGAIASPKGYRGYPNSICTAVNDVVVHGIPTAKPLEEGDLITIDVTVYKNGF
                     HGDKAATVIVPGKKNPEGERLLRVTEAAMYLGIAEARAGARLGDIGAAIQRHAESAG"
BASE COUNT         7862 a        10940 c        11523 g         7782 t
ORIGIN      
        1 cccgttgccg acgtcggtga cttgaccacc agaatcagct tcggccgcgg gaaccgcata
       61 tactcttgaa tggataccgc gtacagatag ttgaacgcgt cgggtttgtc gaggcggtag
      121 cgcggtggtg atgacgtgtc gttgccgcgg tacatgtagc tgtacgcatc cgcatacagc
      181 gtgccgagtt cgttcgtcca gtcgatcgtg agcatggaac cgccgtgctt gcgagtgtgc
      241 gtaagccacg accgcttcag ttgctgcccc gagccatcgc tccactcgtt tcgcggaacg
      301 ccgcccgggt agcacatgag tgagctttcc gcgggctcgt tttccgggtt cgtccagaat
      361 ctgccgagat ggaattgcgc ctgcaggcca ttcgcgaaca cggccggaac cgcaaccatc
      421 ccgagcactc cggcgaacag gactcctttt cgccagttca ttcctgaaac tccttttctc
      481 ctcacgagtg aggaaccctc aaccagcgca attccacaga cgtggagttg catgaaaacc
      541 acgtgctccc gctaccagca cacctccttt caatcgtcga gacatccaac gtgcccatga
      601 gaagcattac aaatcgaaac gaacgcccac ccggtacgag cgtgggtaaa gatgatgcat
      661 ccattcggtc tgcgcgctcg tcgggcgctt ccagttgccg tccttgtcct tccaggggcg
      721 cgtgaacatc gcaaaggtgg actcatcgcc gaccttcatg tcattcccct gcagacgctg
      781 tccgtaaatg tagccgagat agtcgatgta gttctggatc cctccgccat tgaggcgttt
      841 gatgttgaac acgttcccga tgtcgaggta cgcggacaat gatctgccgc ggccaagctg
      901 gaacgccttg ctcagcttca ggtccgtgtt gtagctgctt gccgagggaa ggatgttttc
      961 gtccggaatc tcccgacgtg gcaacgcttc ggggttgtag attacctcgc ccccggaggc
     1021 gtacgactga acgacactaa ccgcccaccc gccgcggatg ttgccccagt ccatcggcgt
     1081 tcccacgcga agcattccct ggaaataccc tttcgggtct gaagtcgacg cgaaggattc
     1141 aaacggctgg atgttgacgg tattcagatc cacaacggtc aggccgacca taccgctcct
     1201 cgaactggtt ttctggtacg taagccaacc gttgacaaac cgcccgctgc ttcgggaaat
     1261 cttgatttcc gcgccctgaa tgtcttcgta gttggcattc ctgaagattg aatacccgcg
     1321 aaaatccgat cgcagtgtgt tggtgacgga aatacgcgtc acctggtcca cgttgtactt
     1381 ggcgtaccca cggaccgtca tcaccagccc tagcgggaag acgtgttcca cgccgacttc
     1441 gtacatcgtg gtcttcgcgg gtcgcaggtt ggggttgaac atgtcggcga cccagccgtt
     1501 gaatgccgct cggccgtcgt ggtcggagtg gccgtacatc acggccgctt tctgcatcga
     1561 gtagaagatg ccgtagttga agaagaactt cgtgcggtag ctaaccgggt gagatatgcc
     1621 gaagcgcggc gcaatccgcc agtgaacgat gttatccgat tgcggaaaaa tgcgcacaac
     1681 gtcccagggc tccggggcga cgccaagttt ctccttcacc ttgaaatagt cggaaacgtt
     1741 gttcaccgga accggcccgt acgcgagcgt gtcccacccg agggctacgg agaggtcttt
     1801 gaaatagcgg gttccgtggt tcacgaagag gtcgttatcg aacatgtcgt tgtagaaata
     1861 tgcgttctgc ccggcatcga accgttcgat ccgaatacct ccgttggcga tcatcccgtt
     1921 cgtttcgaac ttgtcctgca gatacacgcc caagatgctc ggattaactg ccgggtagtt
     1981 gccgccgtaa tcccgccact ctgtgttcgt ggtcagagcg ctgttctgaa tcatgtggta
     2041 ttcaaggtca ccacggataa actcgacgcc cgttttcagc gtgtgagaac ccatcgcatg
     2101 cgtgaaatcg ctccggaaca ctatctggtt tgagcgcccg gtcagccgca cgtggccgcc
     2161 gccgcctatt ccgtacactc cactcaggtc cttgtacgcg ctcatcacat acccttgagg
     2221 cgacacgacc cggcccactg tcgtggtgtc acctccgatt tcccgataga tgaacttcgc
     2281 ttccgaaggg acctggtagt acaggtaggg aatgccgaac gggcttactg gcgccagacg
     2341 attgggattc agggcatcgg ccaggctgct gggtttatcc gggccgccca ccccgttcgt
     2401 gtcagtccat ccgaatgcga agcccatcca gccctgaccg ctccacgtgt tgggtggctg
     2461 atagcgcccc cccggagcaa agtcactgaa gtccgcgcgc ggaagatcac ggttcacttc
     2521 ccatttcgac gaagaccggc ccagcgaggc actgaggaat gtcttggggc tgaacgtgta
     2581 ggtgaaggtc ccgccgtact gggagtattc gccattgaga agcgagttgt acgagaggtt
     2641 gaacttggtg ttgtcgcttg tgccggtcag accattgagc agatcgccgc tcgccctcaa
     2701 catcaccgga tcgtttccgg ttacgctggc tccgcttgcc gccagctcag ggttcgatgc
     2761 cgctccaccg gtagaaccgg cagcagttcc ctcgttccga ccggtcatgt aactgaactg
     2821 aattttgatt ttgtccaatg gcgtgagcgt gaatttcgct tcaaatgtct ggtcctcgtt
     2881 gtagggcctc agcgcgggaa ccaccgtcat ctcgtacgtg cgggagtagc caacaacaac
     2941 gccacccatc cttttcggaa gcgcccaccc cgccgccaga tccaggttgt aatcaggatt
     3001 gttgctgtac ttccacacct gttcgttcat gttggcttcc cattcccagg cctctcggac
     3061 ctcggcggga gtccagtctc gtttaccgaa ccagccgcgc gcggcattca caaccgtcgc
     3121 gcgttgcttc cagccatcga atacgcggaa cgcgcttgtg cgcgggttca cgtacttcgg
     3181 attggtcgga tcggtgaatt ccggtgccgt gcgcgtctct tcatagagca agggccagta
     3241 cagcgggcct gccagagcgg aatcggcaaa cggtgaatcg ctgctcatga gccagtaatc
     3301 atattgatcc gaatcgtacc ctccgggacc gaaatgcttc ttcctcgccg gggccatatt
     3361 gtacacacca gacacccacg gcaatgcaac tgccgcgctc cggcgttccg tgccgtcctt
     3421 gttgaccacg ttcaccatac cggaccggac gtttccgtat tccgcgttga atccgccggt
     3481 gagaagcgtg acctcgctga caagaacctg gttcaactgg gtgtagggct tgctggtcag
     3541 gccgtccgta cggtccagac cgtcaacctg aaaacggatt tccgacgggc ctcctccgcg
     3601 aatctccagt tccgttccgc gcgtaacgct cacgcccggt tcgtagttaa ggaaatccag
     3661 cattcgcgat acaggcgctt ccgaaatccg tgcggcattc acaaccgtct gcgaagacgt
     3721 gacgtctact tcgatagggg ggcgctcggc gcgaaccacg attgcgccca cctctatggt
     3781 agcctcggtc agacggaagt tcacatcggt cgttcggtcg gcgctcacac tgaccgacgt
     3841 gatgctggac cgccggtaac caatgaggtt cgcagtaacg ttgtgcactc cgggcggaac
     3901 ctggagaatc atgtaacgcc cgtcggggtc ggtcgtcgcg ccgagccggg tgccctccag
     3961 cgtcactgcg acccctggaa gtccagcacc gttctggtca gtcacacgtc ccgcgatttt
     4021 ccctgtagtt cccgcgcgca cgcccggcac cgcaaggaac cccatcaaca tcagcgagaa
     4081 aaccgccgag acatctgcca aacgtcggat ggcgtgcacc atgctccctc ctgtaacact
     4141 cccgcgcaac gagttccgcg tgctcgaatc gagaagcctc gttgcggaaa cgcaaacgta
     4201 tcgcaccgcg ttcagattca gagacggcgg aaaggggaag ttgagcattc cggaacaata
     4261 ggctgtccac aatggcgacg aatgctggtc aagaaagccg tgtccacctc gccgacgggc
     4321 ccctcaagcc cttgacacgc agtctcgcga tcagtgccct cgcgccgccg ttgcgttcgt
     4381 ttccggatct cctcttttcg tcctcatggt gaatgatata cccaatcccc accgcggtgt
     4441 caaggttttt tttcagattg atccggccgc aaaagtgcgc cgcatatttc gtaaactttt
     4501 aatacgtggg caaacagcgg caatgacgtt attttgcgca ttgacagagg atcgccgcac
     4561 cgaagttctg cccgaatcaa aagagagaaa aatcggccgc actcgttcat ttaatacttg
     4621 aatccgtccg ctctccggcc cggtgttgtg tgcccgtctg ggagacgcct atttttgtgt
     4681 tcagcgtttc acgcccggtc gcaaggtcgc agaagaatca caaggaactg caatggtacg
     4741 agttctcgga ggactcctgg ccgtcctgtc ggctgttctg tctgcgaggg cctccgactg
     4801 ggcgccgggc gccggaatca acgttgttac ggcacacgtg gttcgaggcg ataaacgcaa
     4861 cggattgtcg ctgcaacccg cgttctggtt caacgcgttt caggagcgtg gtcttttcac
     4921 ttcctgggct tcggttgcgc ccgccgaccc ggccctcgac gatctggtgc tcgacttccg
     4981 ggccggggtg ccgaccgctg gctggggatg gatgggcgga gttgtgcact gggattggag
     5041 ccgcccgccg tctgattcgc tgcgctcaca ttccctcgaa ataggtccga ccctgacatg
     5101 gttcaagccg ccgtaccgcc ccagccttac ggtgacgtac gactttgcgc agcggaacgg
     5161 ggtccttttt acggtgaacg ccccttacac cgttcgcatg acatggttac cggcaacgac
     5221 gttcaccccg gaggttgcat ttcgttttgc gggagcgggc aatcgtgtga gcgctgagta
     5281 tttctcgttt tccgcgttgc ttgaacgcaa aatccgaggg gttaccctgc tgccgacggc
     5341 gggaatcgtt cctagaagcg aactctcgaa atccctggtc tgggcaggaa ttcacgtcaa
     5401 tgtggttaga tagaattgct ccgttcgcat ttgttttcct gggcgcggtg tttttcgggt
     5461 gcgcacccac atgcgtgaaa acgccgcccc ctccggcagg cgaactcatt gcggaagtgc
     5521 tgtctgttcc gggcgcacac gccatacagg cgtacggaac gctgcgtgtg acatcacccg
     5581 aagggaatta cagcggatca gccgtcgtgt tctacagcta tcccgaatcg gtgaaagttg
     5641 ttctgcaggg aggatttggc accaccttcg ccgaaatcgc gcttgcagga gggaagggaa
     5701 tcgcatatct cccccagcaa cggcaggcac tggaactggc agacggaagc ggcttgctgt
     5761 tcgggtccgt agtggtctac ccgtccatgc tcctgcatct cctgaagccg gttgatgccg
     5821 aacggctcgg acaggatgcg ataatcagca cggagtgcag ccagtacgtc cttgcctctc
     5881 gcactgccgc aggaacgcgc atctggcgcc tctcggcacg tgcgcgccgt ctggaggcgg
     5941 aggagttccg gtccgtagag aatacgatgt tctggacccg gacgttcggc gcggcgaacg
     6001 gcatccgcgt gccgaaatca ttctcagtcc ggttgggaga aacgaccatg agcgctgaat
     6061 ttacgcgcat caacgtgtcg ccggtgatgc gacccaatac cttcgccgtt gcgttgcccc
     6121 ccggcatcga ggtgatgact gttccccgcg agtaatcggg ggcggcatgc agaaaccacg
     6181 tcaccgggca aaaggtaggt ccgtttcctg aaatcgcggg gaactgaacc aggaggaaga
     6241 tccgtgctac cgaacgttcc gtccgatcca ggcgccttgc atccgcttgt cgtacatttt
     6301 ccggtcgcgc tgttgctcgt tgcgccgctc ttcctgttga tgggggcaat ctccaaaaac
     6361 gacaggcctc agttgcgcag ttgtgttctg gttctcatgg cactgggtgt acttgccgcc
     6421 tgggtggccg tatggacggg ggaacacgcg gcaaccatcg cgcgaatcac tgcgccgcag
     6481 gccgcctcgg tcatcgagca gcatgaggaa tccgcagagg caacggcggt aattttcacc
     6541 gtgctgctcg cggtattcgc catccgcgtc gcggtagact atttgaggaa agagaacacc
     6601 gttcgacgcc cgatgcgcgt gttctgcatc gtgttcctgg tggtgtactg cgtgtgttcg
     6661 ctcgttgttg ttgatgcagc gcatcagggt ggacgccttg tgcaccagtt cggggtacgc
     6721 gcccagatgg ggagcgcgcc ccccggaggt acgaacccct gagacaacaa cctccggcct
     6781 tcaggcgccc ccaatacaat gaaaccagga tggcaaaggt ctcttccatg ggacattcga
     6841 aagcgatggt actttcacac gacgcggaat ggcgttggca cgtacgtgcc gacatgccgc
     6901 cgcctcgtgt cggtgaggtc acccacctgc tcaagccgca cggggagtgt gtactgacga
     6961 ttctgacttc gcagcaatcc gtctggttgc acacgaagga cttctacccc gctggctgtt
     7021 accggttgcc caccgggggc ataaaaccgg aagaacaccc ggacggggcg gcccgccgcg
     7081 agctgcggga ggaagtgggc tgcgcgggtt catgcaggat actgcgcctc gggaaaatca
     7141 cctacgtgga agcgagtgga actgcctacc cgttcgtaag ttatctatat caagtagccc
     7201 cggtcgaaaa gacgccaact tcggccgacg aatcagaacg catatcggga tggaaaaccg
     7261 ttccgatttc gcaccttggt gctgtagcgt ctgaactgcg tgcgctaccc gccgaatggg
     7321 tagactgggg ttgtttccgg gcggttgccc acgatgccgt gcgggaaata ctgcggcgtg
     7381 aagcgcagtc cccctgcctg tgagggaaca tgatacaaaa cgagttgctc cagacgctca
     7441 aaaccgaggc gtacaccgcg gtggtcgcgg acgttcttga cagcctcggt ctgcagaacc
     7501 agattctgcc ctcgcgcatc cgcccactcg gaaatagcga ggcagtggtc gggatagcgc
     7561 gcaccatcct cgtccgggat gtggtgtcgg cgtctccaca tccctacgaa gtcgagtttg
     7621 ccctcgtgga cgacctgcgc gaaggcgacg tcatcgtggc gcaatgtgga gagcgcgaag
     7681 cggcgttctg gggcgagctt ttgagcactg ccgcggcgaa gcgcggcgcc gtgggcgcgg
     7741 tggtggacgg cttttgtcgc gaccttcggc aaatttcgga attgcggttc cccgtgtatg
     7801 cccgcgggat gagtccggcg gactccaaag gccgctgcga agccgttgca agggatattc
     7861 cgatcgaaat cggtggtgta gcgatacacc cgggagacat gattttcgca gatctggacg
     7921 gcgtggtcgt agtgcccgcc gggtccatag acgaaaccgc gcgcggcgcg ctggaaaagg
     7981 tccgagggga acgaaccgtt ttcaacgcgc tgcaatcagg catgtcaacg cgcgaagcgt
     8041 atgacaaatg gggaattctg taacgatacg ctgaggagtt caggtatggc cgaaaaggat
     8101 gcaaagctta cccaggagca gcaagacacg tttgcgttca tggcaatggt ggagctctac
     8161 cagcagactg cctggatggc tttgggaaaa atcgccgacc cggctacgaa agaaacgaag
     8221 gcgaacctcc cccaggcacg ctgggcaatc gacacgctcg caatgatcga acgcaaaacc
     8281 cggggcaacc tttctgatca ggagctccgc tttttgcgtg agatcctcca catgttgcgc
     8341 atgaattatg tggaagaagc gaacaaaccg gttggagaac caccgtcaac tcaggacaaa
     8401 accgaaacga aaccggaaaa ggaaacagcg acgcacgacg aaccggaaac acctccgccg
     8461 gcggaaccgc agaacgaatg aaactcggac cgctcctttt cgaaccgata taccgatcgt
     8521 atgtctgggg gaaccggaat ttcgagcgcc tgttcggccg cgttcttcca ccagaagtgc
     8581 cgaccgcgga atcgtgggag ctttccgacg ttgacggtgc gatttcccgt gtggaatccg
     8641 gtccattcgc cggccgttct ctgcgggagc tttgccagga attcgccgcg gacatgttcg
     8701 gcgagcagag tggctcttcc atctgcggcg gtcgcggatt tcccctgctg gtgaaattcc
     8761 tcgacgccga gcgggtgctg agtgttcagg tgcaccccgg cgaccagttc gccgcggaac
     8821 acgaaaacgg gtctatcggg aaaaccgaga tgtggttcat cgtcgaagct cgcggaaacg
     8881 ccgaggtgat agccggtctg cggcctggaa ccacgcgtgc ggagttcctc gaggccgcgc
     8941 agtccggcag cctcgaacca ctcctgcagc atcactgtgt ccgcgccggt gatgtcgttc
     9001 ttgttccggc aggccgcgtt cacgccatgg gagccggtat tgtggcgctg gaaatccagc
     9061 agacgtcgga tattacctac cgactgcacg attggggacg cgttggaccg gacggaaaaa
     9121 cgcggccgct tcatctggac cgggcgatcg aagtgattga tttctcggac gaaggacgcg
     9181 cagttacgca accggttcaa cgaacggagg actggggcgt ttggtcgttg ctcggagtca
     9241 ctctattctt cctcacggaa tcgttctctc agttccggtc ggttccgtgt accacgcgtt
     9301 ctggcagtcc ggatattctc gtggcgatga acggaagtct gaggatttcg tgggaccacg
     9361 gtgacgtcat cgttcctcca gggcgaacgg cattggtacc ttcgtgcgtc ggtctctacg
     9421 tgttggaggc gaccgaagac acactggtgg tgcgctcccg ggtgcctgat cgttcggatg
     9481 tgctgggcga ctggacaccg cgaagcaatg aggaacggac ggccgttctc gacgtatgtg
     9541 acacgcgggt tgcggagctt gtcaccgcat aatctggccg gcgcgtgtaa tctgggatcg
     9601 tctgttggtc acgctgattc ctcgcggagc ccaaatggca cgaacgcgct tcatccagac
     9661 cgtattcggg aaaccccggc aattccggcg ttgggggttg ttgctggtct tctgcagcgg
     9721 ctgtctgttc ctcggcgtgg caagcgctgc ttccgaggta catgcgccac gcaaccacac
     9781 agcagcagtc cctccaaggg gagctggctt cgcatcaacg ggtggcggcc accaaactgc
     9841 tccgcagacc ttggttggga ggatttttcc gacaatgttc tcgcttgccg ggtccgcggg
     9901 aggcgttgta gtcctggctt tcgtcggcgc ggcgtttttc tcacgccgcc acggccgcaa
     9961 gggttctggc tcaagcggca caccgcgcat ccggaccgtg tcaccgggcc cctccggttc
    10021 cgcgcccgag ccggcatccg cccgaacggt gatcgcggcg gtcgcaaagc cgttccagtc
    10081 tctcgcggca ggtgtcgtat cgctgaagcg ggaggtgtgg aaggaaaaca tcccgcgact
    10141 ggcaggcatg gtgtttctcc tgctggcggt cggtgcgctg gtagttggcc tcgtggaggt
    10201 cctgttcggc gccgaaaacc agcgagcgaa cttcggcagc cccatcaatg cgctttactg
    10261 ggctgttgtt acccttgcga ctgtggggta cggggacctt gccccgcaga caacggcagg
    10321 gcgcctgctg acttcggtct tcattctcgt aggaatggtt accatatccg tgtttaccgc
    10381 gacgctcgct tcggttttca cggcgaaaag gatcaaggag gggcggggat tggaaaaggt
    10441 caaggctacg aaccatgtgg tcgtgtgcgg cactggacgt cacctcgaca cgatcatccg
    10501 gtacctcacc cgcgcgggga ttaccagaaa acaaccgatc gtgctggtca actcgctgac
    10561 cgaagaagcg atgaacgaaa tcctctaccg ctactcgcac ctggatattc aacatgtgca
    10621 cggggacttc actcaggaaa gcgttctgcg gagggcgaac atcgaagatg cggtcgccgc
    10681 gatcatccag gcgggcgacg acactcagga tcgttcgcgc gccgataacc gcacacttca
    10741 ggcggcactg gcaatcaagg cgctaaatca cgatatcaaa ttgaccgccg aggcgctcga
    10801 tccggaaaac gaagcgcacc tccgccgggc gaacgttgat gacgttatcg tcagcggcga
    10861 gttctccggt tatctgctgg gagcatgtac tgtgtcaccg gggttggatg ttgcgctgcg
    10921 cgaactcttg accgtggaag tccggaacga catgatccgg aacccgatcc ccgaatcgtt
    10981 ccgaggcaag acttccaaag agctgagtga gtggtttcga cgccgagagg ccatgctgat
    11041 agggataatc gttgaacaga aagtgctgaa cctggatgaa atgctccagg acgatttttc
    11101 gtccatagac cggttcatcc gttcaacttt ttctgccgcc ggaaaagaac tggtggcaca
    11161 aggcaaaggc agaacagagg ttgtggtcaa tcctccagac gaccgcgtaa taggccccga
    11221 cgatgcggct atcattattt cgccgcgcgt gattcccgtc acagaaacat gaccaggccc
    11281 atttccgatc gggggagtac ctgatgccgg gatacgagac tctgaaaaca gcttccctgc
    11341 tcaagggatt gaccgattcc cagctgaaga agctctggga tgctggcgaa agcaagacaa
    11401 tcaaagccgg agacaccatc atccgggaag gcgaagtcgg cgacaccatg tacgtcctgt
    11461 tcgaggggac cgtggaggtt tcccaggtac tggtgttgcg acttgggaac gctgccatcg
    11521 ccgagaagga caagacgctg atacgccttg aaggaacagc ccgcccttgt tttggggaaa
    11581 tgtcgttgct cgaagacgct gaacggtcag ccacgatcac cgcactgacg gactgccagg
    11641 ttttcgtcat cgaccggagc agcttcaacc gactggttga ggaagacccg gtgatgggta
    11701 ccaaattgct gagaagtatt gcggtcgttc tttcaggtcg tctgcgcaaa gccaacaagg
    11761 atgtcctgaa acttactacg gcgctaagcc tcgcgttgac agattgaatc atccgacact
    11821 cgcccggaca cgccatgagg ccgtcactgc cagcccgttc cgccggagaa gatcggggct
    11881 gatgcgcgaa cgcttcggct tcatcgcgag tgcactcgcc gcgttccttt ttgtcggctg
    11941 ccccgcccct ccccgttatg cgcctacctc tcgtttggct ccccgtccgg gggaacttcc
    12001 ggttcgcatc ggccttgcgg aggcgacagg aagcgtccga atcgaagccg atcaggggct
    12061 gcgtctcgtc gccggcgaaa ccgtgcgatt ggctccatcg tttgcgctgt ctccctctcg
    12121 cggccatatt gccgtgaacg gcgcgaggac accccacaag cgtgttacct gttttgcatc
    12181 cactcccctg agctgcaacg gcgtaccgta ccgtggccag ttggaggttt ccgcggtcga
    12241 gggtgcgttg cgtgtggtga atatcgtggg tctcgaagag tacctcgccg gcgtggttcc
    12301 cgcagagatc gggcgcctca gcacccgcga gatcgaggcc gtcaaggcac aggcgattgc
    12361 ggccagaaca tacacggtag caaaccgggg gcgtcgccgg gcgcaggggt tcgacctgta
    12421 cgccgacgtc cgcgaccagg tttacatggg tgttcgaggg gaacacactg tcgcgacgcg
    12481 tgccatcgaa cagacccggg ggcaaatcgt gacgtaccgc ggcgagccca tacaagcgtt
    12541 tttccacagc acgtgcggag ggttcaccgc cagcatagaa gacgtctgga acgctgaccc
    12601 gctgccgtat ctgaagggcg tgccagaccg cgactcccac gggttccatt gcgaaacgag
    12661 tcgccatttc cgatggtcag aagagtattc cccgggtgtt ctgcgcacga ttctgtctga
    12721 aaacctgttc cgtgaggtac ccaacgcacc gcggaagatc ggcaccgtaa ggaaaatcga
    12781 agcgccggaa cgatcttcga gcgggcgcgt tcgatatctc aaggtggaga ccaatcgggg
    12841 tcactggcgg gtctccaaag accgcattcg ctgggcgctc aggcgccctg tggacggcaa
    12901 cccgatactg agaagcacct acattcgtat cttttctgaa tatgacgcgc acggctcgct
    12961 tcagcgagtg gttataagcg gcgccggaaa cgggcatgga gtgggcatgt gccagtgggg
    13021 cgcaatcggg atggcgcgga aagggtaccc ggcggagcag atcctgaagc attactatcg
    13081 aggcgtcacc attaccagca tgaggatggc agattgaaga cacgaccggg aggtggagca
    13141 gcaatgcgta tctcgtcgct cgcgacaggg tgtatcgtgg caatctcgtt cgttgcgctc
    13201 ggatgcccgg cgccgccgcc cgcaacggtg cctgcggcgg gtgatacaca gaacaacgat
    13261 accacggcaa ctacaactgc tccggaactg cctgatccac gcggccagct ccctcgcacg
    13321 gcaacgccgg ctcctgccga ggcgaccgat gggccggacg ccgaacccgc aagcgagctg
    13381 gagtccgcag tttattccgt tcaggtgatg agctcttcct cgcgagatgc cgctgaagcg
    13441 gccgcacgac aggtccgctc actcacctcg caaccggttg aagtggttca ggaatccggg
    13501 ggagcctggc gggtatacgc gggcaggtct ggaacgcgga ctccgatgga ccggctcagg
    13561 gatcaattga tttcggcagg gtattccggt gcgtggacca aacaacgcgt ggtggaaacc
    13621 tcggtacgtg acaatactat cccaactggg gaaagcgtgt acagcgttca gattttcgtg
    13681 tcatcgactc cggagaatgc acggcgggtt gccgctgaag ctcgttccgt aacccgcctt
    13741 ccggtggaag tggtggaaat ggatgggtac tggaaagtgt tcgtcgggcg gagtcccacg
    13801 cggagtgcaa ttgacgccga gcgcgatgca ttgcggcaac gaggctaccc ggacgcatgg
    13861 acctaccgcc gccagggaac acgatgaaac gccattctgc ggttgtttct tggcgatttc
    13921 ccgtggtaca tttgcacctg cgcgggtaac tggcgcatga acttcgacct gaacatcggc
    13981 aggtacgttg cgggaacaag tccgatccac cggctcgacc cccggacgaa actcggactt
    14041 gcattgttcc tttgcatcgg cattttgcag ggaaccagcg ccaccgcaac ggcgtggcac
    14101 tgcgtgctgg caacggtcct gttcggcatc agtggcctga aggcgcggtt tgtcgcgaga
    14161 aatctccggc cgttcgtgta tcttgccggc atgattctgg cgagccacct cctgatcaat
    14221 ggcgcgggca gctggcagac cggaatctcc gtcggtgtgc gtcttctcgt catggtttct
    14281 gttgtctcgc tcttcagctg gacgacacag cccctggcga cggtggcggg gttacgtcgg
    14341 ctgggctcgc ctctggcgag gctgaaggtt ccggttgatg cggcggcaac ctccatcgga
    14401 ctcgcaatgc gcttcgcgcc cattgtggtt tcggaagcgc agacggtgtt gcgtgcgcag
    14461 gccgcacggg gtgcggattt ccgcggcgtg aaaaggaaac ttacgctgat tgttccgctt
    14521 ctcggggcgt tgttcgatcg ctcgtttgcg cgcgccgagg tgcttgcgga agcgatgcag
    14581 gcgcgagggt acgatccgtc agccgtcagg acatcgtacc accgactcgg gtttcgaggc
    14641 gtcgatgtga ttgccctggc gtacgtggca atctggctcg ggggcgcggt catcatggag
    14701 cgttatgcac cgtgagtact cgcctgcgcc tcgacatgga gtacgaaggc actgaattcg
    14761 cgggctggca ggttcaaccg gggttgcgaa ccgttcaagg cgagctcaac actgctctgt
    14821 cgaccatatt gagaacgccc gttcgcgtta tcggggcggg gagaacagat gcgggtgttc
    14881 atgcgcgcgg acaggtaggt cacgcggatg ttcctgacgc gtacccgcct cttgcgcgca
    14941 ttctcgcggg tgtcagagcg ctgaccgggc cggacctgct tgtccgggaa ataacggttg
    15001 ttccgggaga cttccatgcc cggttctccg cgcgggcgcg gacatacgag taccggatcg
    15061 cgctggtgcc atccccgttg gagcgaaggt tccagtggtt tgtgccgtac cggcttgatg
    15121 tcggctcgat gagtgtcgcg gcgcagaagc tggaaggccg atacccggcg acctgttggt
    15181 gctcatccag cgcgccggac acgacagcga ttgtgcatgt ccaacgtacg gaaattgaac
    15241 gcagggcaac tgaaatcgtg ttccgcatta cctccgaccg cttcgtgacg cacatggtgc
    15301 gaacgatcgt aggtacgctt gtggaaatcg ggcgcggcgt tcggagcccg gaggatatcg
    15361 gcgcaatcat ttcttcgcag gaccgtgcgc gtgccgggcc gaccgcgcca gcccacggcc
    15421 tgtgtcttca atccgtggaa tattgagcct atggattact cgtacggtga gtgggagcac
    15481 caggatcaac cccgccgcac ccggttctgg accggagttt tgcttggtat cggcgtcagc
    15541 gtcgtggcgc tgttcattgc gaaccgcata actggacggc attggggcgg gcgcttgtcc
    15601 gttccggagc cgccggccgc gttcagcctc gtttccggtg tgggtgacac caccggcgac
    15661 gtcggcaatt cccgccggaa tgccatagtg cgtgcagtcg aaatcgttgc tcctgcggtt
    15721 gtcaccataa gtgtgacgca gttgcgccga gaacgcgctg ttccgttcgc ggatgacccg
    15781 atgtggcagt tctttttccc ggaacttcgg caggagtttg tgcgccgcgt ccaaagtatg
    15841 ggtagcggtt tcatagtctc cccggacggc atgattctga cgaacgaaca cgtcatccgg
    15901 ggcgccaccc agatcgtggt gaatctgccg gatggccgct ccttcgacgc gaagattttc
    15961 ggggaaccgg atgaacaggc tgatatcgcc gtactgaaga tcgacgctca gaatctgccc
    16021 accgcaccgt tggggagaag cgacgacctc atgataggcg aatgggcgat cgctattggc
    16081 aacccgttcg ggttcctgat ccgcgacgct catcccaccg ttaccgtggg tgtgatcagc
    16141 gccctgaaca gggatttcag tccctcttcg gccggcggac gtacgtaccg ggacatgatc
    16201 cagagtgatg cgtccatcaa cccgggaaac agtggcgggc cgctggtgaa cagcgaaggg
    16261 tacgttatcg gcatcaatac gtttatcttc actcagggcg gaggttcgga aggcgtcggc
    16321 tttgccatcc cgatacagaa ggcgaggcag gtgctggacg acatcgttca attcggcgaa
    16381 gtccgccaca acttctggac cgggattcac attcaggacg tgaaccgctg gatcgccgaa
    16441 agtctcgggc tccaatcaga tcggggagca atcatcacca atgtcgaacc caacagcccc
    16501 ggagcaaaag caggcctgca acgtggagat gtgatcattt ccgtgaacgg caaagcgata
    16561 gccagcgacc gggatgtgcg ggacgaattc cttggcgctg ttgtcgggca acgcgtggaa
    16621 ctccgaatca tcagaaacgg ccgtgaactc accgtgacac ttgtcctgga agagatcccg
    16681 agaaacagga gggaccgtgg ctaaggaagg acttcccttc gtcctgggcg gcgtgtgtgc
    16741 gggaattctg cttctcgccg catggcacgg cctcaggtgg gagccgctcc gtatcctcgg
    16801 cattctggca ctggtttttg gagcattttc gctgttcttt tttcgtgacc cgcgccgaac
    16861 acctcccgcc ggtgaagggc tggtgctgtc gccggctgac ggcaaggtcg tggaaatcgt
    16921 tcaagaagac gacccttatg tgggcaccaa tgcaatccgg gtaagcattt tcctgtcagt
    16981 tctggacgtt cacgtgaacc gaaatccgat ttccggttcc gttaccgcga tggtgtaccg
    17041 ccccgggaaa tacctgatgg cgttcaacga gaaagcatcg gcggataacg agcaaacgca
    17101 catcgccatc aaaggcgagc ggggaaccgt tgccttcaaa cagatagccg gcttcatcgc
    17161 gcgcagaatc gtttgccgac tcaaagtcgg tgacacggtg cgcgcgggag accggtgcgg
    17221 actgattcgt ttcggaagcc gcgtggacgt cattctccct ccggatgcga gcatccgtgt
    17281 cgcggtcggc cagcgcgccg tcggcggcaa gactgtgata ggagtgcttc catgatgccg
    17341 ctcgttacgc aggctcttcc gagcgtcatg actctcggaa gtctgttttg tgggctgctt
    17401 gcagcaatca aagcaatgga aggaacagag gaggcgtttg tcactgccgc gtggttgatc
    17461 attgccgcgg gacttttcga cggtctggac ggcaaggtat cccgcatatt caaaactcag
    17521 agccacttcg gtgtagaact ggattccata gtggatgtgt gctctttcgg ttttgcgcct
    17581 gccttattga tctaccagtt catcctgctc aacgcgtcgg gtttttccgg gatcgctttt
    17641 cctgttgcgt tcgtctttct tgcctgcgga gcgctcaggc tggcacggtt caacgctcag
    17701 ctcaaaggat tcgacaaagc ggccttcaaa ggtgtaccga ttcccgccgg tgccggtatg
    17761 gtagcgtcct ttgtggtgtt ctcccgcagc gagttcatta ttgatctcgg catagactgg
    17821 gtcctcccct tcctcgcgtt cggaatggct ctgctgatgg tcagcaccgt cagatacgac
    17881 aaaacgccgc gtttttcctg gcacggacca acatctgaac gcgtgcgcct tgtcgtgatg
    17941 atcgcctttt tggtcatcgc agtatggtat cccgccgagg cgttcttttc gttcggggtg
    18001 tactacgtcc tgtatgggct ggttcgtgcg ctgggaaatg catttgttcg acacccggcc
    18061 gtcgctccgg ggcagccttc cctggaacgg gtcaaggagg ggagcaggag atagatgcct
    18121 cgttaccgca gtactggtgt cctgtttgca agcctgctca tagggcttct gggtgcgacg
    18181 gttctgagcg ccgccctcag cctttttgtg ggtcccgcca gcgtcgtgca cagagtcctg
    18241 atcaacagct tcacctacga ttccggagtg ctggtcctga atttgatcgt ggtgacgatc
    18301 tctttcggca tcacgttgaa tatcaatctg ctcaccattc tcggactcat gtccgcgttc
    18361 tactggtgga aataccgcct gtaacacccc gttgcgaaag gtgtcctcca tgataggcgg
    18421 ctgggaatgg gtaatcataa tagtggtcgt gctgctggtt ttcggcgccc gccgcatccc
    18481 ggaacttggc aaatcgctcg ggcagggaat ccggtcgttc aaagaggaac tggcgggcgg
    18541 caaacagcgg aaagacgagc ctaaggatga ttccagctcc gggcaatcgt aatcccgctt
    18601 ctgtggtgtg tcagtcaacg ttctgagccc tcaacacagg cgtaatcctt tgaataatcc
    18661 gaatttcacc gctgcggcac ttgttcctca gatcctggaa ggatcggtgc gggcaaccga
    18721 cgtcctggat accgtgctcg ccaccattcg ggagcaggaa cctcgcgttc acgcatttct
    18781 tcaggtcaca gaggacctcg caaggtcaca ggctgaacag gtagaccgca agatagccga
    18841 gggaaaaccg gtggggcctc ttgcgggcat ccccgtcggg atcaaggata atctctgcgt
    18901 gcgcggtggc cgcactacgt gtgcctccaa aatcctcgaa ccgttcgtgg caccctacga
    18961 tgctacggtg atttcccgtc tgcgggatgc ggatgccgta ttcgtcggga aaacgaatct
    19021 ggatgagttc gccatgggct cgtcgacgga gaattccggg tttggtgtga cgcgaaaccc
    19081 gatagacctc gaacgagtgc ccggagggtc cagcggaggg tctgccgcag caattgcggc
    19141 aggaatgtgt gttcttgctc ttggcagcga taccggggga tctattcgcc agcccgccag
    19201 cttctgcggc atagtgggtc tcaaacccac gtacagccgc gtatcacggt tcggtcttgt
    19261 cgcattcgcc tcatcccttg accagatcgg gcctatggcg cagaccgtgc gcgacgctgc
    19321 catgctcctc aatgtaattg cgggagcgga cgcgcacgat tcgacctgct ccccggatcc
    19381 agtcccagat tacctcgaag ggttggagga tggggtccgc ggtcttcgtc tcggcgtacc
    19441 gcgtgagtat tttggcgaag gcctcgctcc cgaaataaaa acgggtctca agcaggtcat
    19501 ccaccggatg gagcgagaag gcgcggaact cgtcgacatt tcgctccccc atgcacctta
    19561 cgcaatcggc acctactacc tcatcgcaac atccgaagcc tccgccaacc tcgcccggta
    19621 cgatggcgtg aagtacgggt tccgcgcgaa ggacgtcaaa aacctcgggg agatgtaccg
    19681 acggacccgt tccgaaggat ttggcgccga ggtaaaacgg agaattctcc tcggaacgta
    19741 cgcgttgagc tcggggtact atgatcagtt ctacgtcaag gcgcaaaagg tcaggaccct
    19801 gatttcacag gattttgcga gcgcgttcgc gaaagtggac gccatcctta ctcccaccac
    19861 gccggacacc gcattcaagc tcggggagaa aacctcagac cccctgcaga tgtatctcga
    19921 ggacatttac acggtaagcg cgaatctggc gggaattccc gggatatccg ttccagccgg
    19981 cagagatagc tctgggttgc ccttcggaat gcaggtcctg ggcccacaat ttggagagcc
    20041 tgtcgtgctg cgcgtcgcac gtgcggtgga aatgctgcgc cccggcgagg caggttgaac
    20101 ttgtgacgag aaatggacga tgagcatgtc gaattaccgg gtcgtgatcg ggctggaaat
    20161 acaccttcaa ctggccacga agacaaaggt gttctgtggc tgcagcaacg agttcggagg
    20221 tgaaccgaat acacactcct gtccggtgtg cctcggcatg ccgggtgtcc tccccgtgtt
    20281 caaccgacag gtgcttgagt acgcgttgcg ccttgcgtgt gcgttgggtt ccagagtgag
    20341 tgaacggtgc cgtttcgtgc gcaaaaacta cttctaccct gacttgccaa agggatacca
    20401 gatttcccag ttccacgaat gtctcgccta tggcggcgcg gtcgagattg acgtctccgg
    20461 cactaccaag cgcatatgcc tcgatcgcat acagatagag gaagacgccg gaaaatcgct
    20521 gcacagcgag gattcgcaca tggaggacac gctggttgac gtcaaccggt gtggtacgcc
    20581 cctgctcgaa atcatcagca aacccactcc cggctccccc gattgcgtgt acgatccatc
    20641 cgcgtacctc aattcgccgg acgaggcaat cgcatactgg gtaaaactgc gtcagctcac
    20701 ccgttacctc ggaatctccg agtgcaacat ggaagaaggc aatatgcggg ctgacgcaaa
    20761 tatctcgatc tgggacgaag cacggggatc ttttggcacc aaaacggaaa tcaagaacct
    20821 caactccttc aaattcgcgc atcgagcact tactcaggag attgcacggc acatcgagat
    20881 attgagcgcc ggcggcaaag taaaacagga aaccatgttg tacgattcgt cgacggatac
    20941 cgtcgccccg atgagatcca aagaggaagc acacgattac cggtattttc cggagccgga
    21001 ccttgtaccc gtggtcgtca cagaagagtt cctggcacag gttcgaaaga atgttcccga
    21061 gcttccggac gcgaggaaga agcggttcgt atccgaattc ggtctcaacc aggatgcggc
    21121 ggatcttctt tcgtcggaac gagcacttgc cgattatttt gaaggagtcg tgaaatcggg
    21181 cgcagagcca cggacggcgg caaactggat catgggcgat gtgttacggg aaacaaacgc
    21241 acgcaagatg gacgtgtcag tctttccggt cagccctccg cgtctcgccg aactgattgc
    21301 cctggtggta tctggtacca tcaaccagaa cggtgccaag gatgtgttcg cggagatggt
    21361 ggattctgga aagcaggcga cgcaaatagt cgaagcgcgt ggattggcgc agatatcaga
    21421 caggacggcc ctcgcgggcg ttgtcgcgga agtcatgggc cgattcccgg gagaaatcca
    21481 gcgttttcga gccggcgaag tcaagcttat ggggttcttt gtcggtcagg tgatgaaggc
    21541 cacgaaggga cgcgcgaacc ccgcgcttgt gaatgatctg gtccgagagt atctgtcatg
    21601 aacttcagat ggctggcata cagtggcctt gccttcttcc tggtcgcctc tcgggtggaa
    21661 gcgttcgggt ccggagatga cagcggcgcc gcaggtttca agagcctttt cctcgtcgca
    21721 accagtcgtt ccgctgccct tggcggcgcg gactgcgcgc tgtcagccaa ccctctggcc
    21781 gcagcaggca acccatcagc ttcaggcgga attgcctcca gaaccgctgg tatcgcggtg
    21841 acggattacg tgcttgacgt gctgccggtg gttgcgtacg gcgcgtaccc cgctcgccac
    21901 ggcgtctggt ctgtggtcct cggaaacgtc tcgttcggag agttccaacg gaccaatgag
    21961 acggccgcgg aagtcggcac gtttggggca aacgatctct ccctcagagt cgggtgggcc
    22021 gggccgctgc ggttggggat tgtcggcggt gtttcactcg gttggatacg atcgacgatc
    22081 gacgcctatt cggcctctgc gctggtgatg aacgtcggac tccagtggca cgcgccgaat
    22141 cagcagactg tcgttgcggt gtccgcggaa aaccttggag gtgctctaag cagctacgtc
    22201 ggcggttcac aggggctgaa agatgatgtt ccgacggagt tccacgccgg ggtttctcac
    22261 cgcccggccc atttcccgtt gcccctgctg ctggtttctg atttcacgct cccgcgggat
    22321 aacgaagttt ccgcttccgt aggcgcggaa attcgccctt tcgagcccct gttcatccgg
    22381 gttgggtatg cttccctcgt acggtaccgc tccaacacag acagtgacgg cactcacaaa
    22441 tcgggattcg cgttcgatga tcgccacggg tcggcgttcg gagggtcagg gctcgccgca
    22501 ggcttcgggg tcgttcacaa aagcctttcc ctggattatg cctacagcct cgcgggcgcg
    22561 tttggcggtg ttcaccgcgt tgccgccgcg tgggcgtggt aatcaagtta cactgatttg
    22621 cgcgcattct gcacggacac ggcgtgcgcg aaccggtgca tggttcaccg cttcgttgac
    22681 ctcctcacgc cgcagtggta tctttaatat tttgagcaat ctgctcgcag gtaacctgcg
    22741 tgcgggccat gaacgcgaga gtggcggaat tggcagacgc gccagactta ggatctggtg
    22801 cctttcggcg tgagggttcg agtccctcct ctcgcatccc tcgcgcagca caccctttcc
    22861 cgaaggcgtt cgccccgcga cgatccttca tcactggcac ccgactggcc acggcgccag
    22921 cgcgaattga ggaacccgat gaaacttgag gtcactgaaa ccgcaccgtg cagccgacgc
    22981 attaacatcg aagtcgaacc cgacgccata gaagagcgca taacctccac ggtaaaggaa
    23041 tatcgccgta ccagaacaat ccctggtttc cgaccgggta aggcaaccga ttccgtcatc
    23101 aggaggtggt tcggaaaaga catccgcgcc caggtcatcc gcgacctcat ggaggaatcg
    23161 tggcagaaag ctctggaaga gaacaagctt cagcccgtag accaggcgca gatcgattcg
    23221 gtcaatgcgg aaccgggtga accgttgcgt ttttccggaa ctgtcgaggt gaagccacaa
    23281 ttcgacgtgc aggattacaa agggctcccg gcgatccggc cggttcacac ggtgaccgaa
    23341 gaggacgtga cgagacaggt caatttcctg cgtgaacgcc gcgcaaccga gactccggtc
    23401 gaacgccctg cgcaaaacgg cgacgtcgtg gttatcgatc tccagaaact cgatgaaacg
    23461 ggtgtccccc tcatcggcca gaaacaagac aacctccgat gggttctggg cggaaccgga
    23521 tcggtgagca aggatttgga cgaacagatc gtcggaatgg cacaaggcga tacgcggaac
    23581 gtaacgttcc attaccgcga ggatttttat gatccgaacc gcgccggcaa agaggaaaaa
    23641 gcgtccataa ccctcaaaga ggtccgtgag cgaatcgtac ccgagatcac tgaagacttt
    23701 ctaaaggaac tcggagaata cgcgacggaa aatgatctcc ggaaagacct ggaggcggac
    23761 atcaagcgcc ggctcgacgc gctcgctaag cagcaggtgc acgtcggact gaaaagcgca
    23821 cttgtggaaa gaaaccagat tgaagtcccg gagacccttg tccagcgact gcttacaagg
    23881 atgtttcagc agcacaagca acagggacac gagcacgatg agaacgagtt tcgcgcggag
    23941 caccgcgagg acgcgttgcg agaggtccgg gcttcgctcc tccttgaacg gattgccgcg
    24001 caggagagca tcgtcgtacc cgatgaggac gtggacgctc gtattgcccg gatggccgcg
    24061 tccatgggaa cttcggttcg caacctcagg gattacatga tcaacaacgg acagtacgag
    24121 caaatccggt atgagttaca ggaggaagcg gtcctgaact tcctcgagga gcaagcggcg
    24181 attgaggaaa gtccgcttcc gaaaaagtga cgagaaaccg gacagcaatc cggaacacgc
    24241 gcgaacgagt acccgtttct gcaaggagtg aaaaccatga atctggcgcc catgataata
    24301 gaacgtgacg gtcgcggtga acgagcctac gacatctggt ccctgcttct caaaaaacga
    24361 atcgttttcc tgggaaccgc gatagacgat gccgtgtcca atcttgtcgt cgcgcagctc
    24421 ctgtttctcc agtcagaaga cgcgaaaaca cccatcgaaa tgtatatcaa cagtcctggc
    24481 ggaagcgttt ccgcggggct tgcgatatat gacacgatga aattcatcga tgccccggtt
    24541 cacacggtgt gcattggtct ggcagccagc atgggagcca ttctgcttgc agccggcgag
    24601 aaaggacacc ggaccgcatt gccgcatgcg cggatcatga tccatcaacc ctgggccggc
    24661 ggcattcagg ggcaggcgac cgacatcgcc attcaggcgg aggaaatcct gaaaacgcgg
    24721 aaccgcctga acgagctgat ctcgatggat accggacgca gccttgaaga catagcgaga
    24781 gacaccgacc gcgacaaatt cttcagcgcg gaagaagcga aggagtacgg catcatcgat
    24841 tccgtaacca ccaaacagat cgcgaagatc cctgcctgag accgggcagt tcagcttctg
    24901 ggagtacaaa acgagtggcg acacgaaagc gcgaagaaga actgcgttgt tccttctgca
    24961 accgcggcgc ggaccaggtc aagcgcctca ttaccggccc gaatgtgcac atctgcgatg
    25021 aatgcattca gatgtgcaac gaaattctca atgacgagaa gcgtacccgg ccggcgctgc
    25081 aagacaaacc gctcccgaaa cccgcggaaa tcaaacggca tctggatgaa tacgcgattg
    25141 gacaggaacg cgccaaaaga attctcagcg tggcggtgta caaccactac aaacgcattc
    25201 gttccgctgg cgccaacccc gaaaacgtcg agttggacaa aagcaatatc ctgctggtcg
    25261 gacctaccgg caccggcaaa acacttcttg cccagacgct ttcccgcatt ctcgacgtgc
    25321 cgttctgcat cgcagacgcg accgtcctga cggaagcggg ttatgtcggt gaggatgttg
    25381 aaaacatcct cgtgcggctt cttcaagcgg ctgactacga tgtcgcccga gcggaaatcg
    25441 ggatcgtgta tctcgacgaa atcgacaagg tggcgaggaa atcggcaaac ccctcgataa
    25501 cgcgcgatgt gtcaggtgaa ggcgtacaac aggctctgct gaaagtgctt gagggaacta
    25561 tcagcagcat accaccgaag ggggggcgca aacacccgga acaacccctg atccagatca
    25621 acacgaagaa catactcttc atctgcgggg gagcgtttga gggcctggaa acgatcattc
    25681 aggagagaat cgggcggaaa tacatcggtt tcaatgctcc cccggaatcc gcaagaaaga
    25741 aggaagcctc ttcgatcctg catcacgcag aaccggatga tctcctgaaa ttcggtttga
    25801 tcccggagct gatcggtcga ctgccggttg tcgccgcgtt ggatgaactg gatgacgagg
    25861 cgctgatgcg aatcctgctg gagccgaaga atgcgcttgt tcggcagtac cagcacctgc
    25921 tcgggatgga aaacgtccgc atgcgctttg aggatgacgc gctgaagttc atcgtggacg
    25981 aggcccggag acggggaagt ggcgcgcgtg ggctgagagc cgttatcgaa tccgcaatgg
    26041 tggacgtgat gttcgaaatc ccatcgttga gcgggatcag tgaggtggtc ttgacggtgg
    26101 atcctgctgc gcggaagctc aaatcgctgt atcttccgga tgacggtctg cgcaaacaga
    26161 tcgcctgaga gcggctcttc gacctggttt tgtgatgcgc gttgtctctg cggagttcgt
    26221 cagaagcgtt gttgatctca acgagctgcc tgccgggcgg atgcccgaaa ttgcctgggc
    26281 cgggcgatcc aacgtaggga aatcgtcgct catcaatgcg ctgacaggca gaaaaggcct
    26341 tgcgcgaacg tcgaacacgc cgggaaaaac gcgcgagatc aatttctacg tcatagattc
    26401 gagctggcat ctggtggatc ttccgggcta cgggtatgtc gctgccgccg aatcagacag
    26461 agtccgctgg caagcgttga ttgagccgta tcttgagcgt cggaagcagt tgtgcggcgt
    26521 cgctgtgctt gtggattcaa aaatcggccc caccgactta gaccagacga tgatcgaatg
    26581 gttgacgagg cgaggcagga actttgcggt ggtgatctca aaggcggaca ggatttcgga
    26641 tcgccacgcc gccgaccttg cggaatcgtt gaggggcacg ttgcctgaaa cggccccggt
    26701 tctgattacc tccgccaagc agaaacaagg cttgcgggaa ctggaaggat ggatcctgga
    26761 acggctctct ggatggcgga aggagactcc ggtccgggca acatggcatt cggaaaccgt
    26821 atgagggaga cggaacgacc gtgaaaacgc gcctgtatac ccctggccca acaatggtac
    26881 ccgaacaggt atcgcttgac atggcggcgc cgataataca ccatcggacg ccggagttct
    26941 ccaaggtgtt gcagagctgc catgaggggc ttcagtatct gttccagaca aagaaccccg
    27001 tgctcatgct cacctgcagc ggcacgggag cgatggaggg agctgtcgta tcggtcctgt
    27061 cccgttccac cctgacgctg gtcgtccggg gaggcaaatt cggggagcgc tggggagaaa
    27121 tctgtgcggc atacggagtt ccgtttgttc ctctggacat tccctggggt gaaagcatta
    27181 ccgcagagca acttgaagag gcgttggcgg cgaatccggg agttcgtgca gtctgcctca
    27241 cgcagagtga gacatccacc ggtgcgctca acgacatcaa aacgcttgcc gcagtcgtga
    27301 aaaagaggaa cgccctgtgt ctcgtggaca gcatcaccgg ttgcggggtt cacgagctcc
    27361 gcgtggatga ttggggaatt gacgttgcgg tgacgggatc ccagaagggt ctcatgttgc
    27421 cgccgggact ggcgttcgca gcgctgagcg acgaagcctg ggaagcagtc aaacggaccg
    27481 acctcccctc gtattatctc agtttcgcca aacaccggaa atcgtgggag aagacaacga
    27541 caccctggac gcccgcgatc acgttgctca caggactggc gcgcgcgctg gaaatgattc
    27601 ggtcggaggg gcttgagaag ctctgggaac ggcaccagaa catggcggaa gccatgcgac
    27661 aggcggtgcg cacgctcggc cttccccttt tccccagggt ccccgcgaac gtgcagacgg
    27721 taatcgcgct gccggaagga ctggagtaca gccgtttgag caagcagctg ttcaacgtct
    27781 acgggatgaa aacggccggt ggacaagacc agctcacagg aaagattctg cgcatcgcgc
    27841 atctcggtta ccttgacgat ggcgacatcg tggccggcgt ttcgttcctg gagcgtgctc
    27901 ttgccgatgt cggatggaag gtaaattcgg gtgaggcggt tgcggccgct caacgcgttc
    27961 tcgcccgtac gcccgcagca caatgaggcc ggttccatga ctgaattcct tcaccacaaa
    28021 gtgcttatca gtgacgacgt gcacgaaatc tgcaagacgc gcctcgaagc cgcgggcata
    28081 gaagtagact accgtcccgg cataaagggc gctgaactac tgtcggcact gaaggactgc
    28141 acgggtctgg tcgttcgctc ggcgacacgg gtcaccgggg agatcatcgc agcaggtccc
    28201 aagttgcagg tcatcggacg tgccggagtt ggggtggaca acattgacgt ggaagaagcg
    28261 acgcaccacg ggatcgctgt tctgaacacc cccgagggga acaccaccag cgccgccgag
    28321 cacactatcg gaatgatgat ggccttgtca cggaacattc cgcaggcttg tgcagccatg
    28381 aaggccggga aatggagtcg atccgcgttc accggcacgg aggttcgcgg caaggttctg
    28441 ggtgtaatcg gcctcgggaa aatcggtcgc gaagttgccc ggcgtgcacg gggtctcgac
    28501 atgaatgtcg tgtgttacga cccgatgatc tctcctgcgg cagcggaagc gactggagcg
    28561 cagttgttgc cgctggatgc ggtgcttgaa caagcggact atctgacgct tcacatcccg
    28621 tcgaacgcga acacgcatca ccttctggac gctgacaggc tgaacaagtg caagaccggc
    28681 gttcggataa tcaactgtgc gcgcggagag atcatcgaca cggacgcgct tctggatgca
    28741 ctcaattccg gcaaagtggc gggggccgcg ctggatgtat ttgagaacga gccaccggac
    28801 atgacggcgc cgatcatcca gcatccgcac accattgtga cgccgcatct gggtgcttcc
    28861 actgccgaag ctcagcatct ggttgcggag caaatcggcc aactggtggc tgaggcgctt
    28921 accacgggaa cgatcaggaa cgccatcaac atgtttccca tggaccgtca gacctacgaa
    28981 cgcgccaaac cgttcctgcg tctggcggag cgtcttggtt cgttgcaggc gcaagtctgc
    29041 gacggccagc cggtgtccct tacactggag tacttcggag acgttctgga gcacccgacg
    29101 gggcttctca cggcgtcggt gctggtagga atgctcaagc aactcgaatc ggggaccgta
    29161 aacctcgtca atgctccagt gctcgctcag gaacgcggca tccgcgtgga cgaaatgcga
    29221 cggaccggtg atgatgacta caacaacgtt gttacggtga cgtatcaaac gcggcttccg
    29281 tccggggaga cgctcacgcg gcgtatcgcg gggagcgttt tcggcgagga ttcgccacgc
    29341 gtcgtccgta ttgacggtca ctcgtgcgat gtcatcccgg aaggccggtt gctgatcatg
    29401 tggaaccgtg atgtgcctgg cgtaattgga gaggttgcca cggttctggg cacgcacaac
    29461 gtcaacatcg cgttcggttc gtacgggcga gacgtacccg gcggactcgc cgtcatggtc
    29521 tacgtgctcg acagcgacat agaagaaagc accgtggata ctttgcgcaa gctgaaaaac
    29581 ttgcacacag tgaagtacgc cagactggcg tgattgggct gttcggatga aaattgcgtt
    29641 cgcatcggac catgctgcgt tcgacttgaa aaatggtgta ttaccgtacg caagagcact
    29701 cggtcacgag gtcgttgact tcgggtgcgc cacaaaggag ccatgcgatc actccgactt
    29761 cagcattccg gtcgcggaag cagtggcacg gggtgagttt gatggcgctg ttctggcatg
    29821 cggaagcggt cagggaatga cgattggggc gaacaaagta gatggtattc gggcgacgtt
    29881 atgcctcacc ccggaagcgg cccgccttgc cagagcccac aacaacagca acattctcgt
    29941 tatggcgggg aggtctttga cccccgccga agggcaggcg atttttgaag cttgggtatc
    30001 tacgccgttt gagggtggtc gccatgcccg tcgaatggat aagatcgccg agtacgaacg
    30061 ccaacggtgt gcaaagaggt aaccgagcac gatgcgatgc cctttctgcg gaaaactcga
    30121 ggacaaggta gtcgactcac gcgccagccg ggacggagca accatacgcc gccggcgcga
    30181 gtgcctcgtt tgttccgcgc gttttaccac ctatgaagtg gtggaatcgg ttcccgtcac
    30241 ggtgacgaag agagacggca ggaaagaggt gtatgaccgc gcgaagctcc agcgcgggat
    30301 agagcttgcg tgcgcgaagc ggcccgttac gcaggagcag atcgaacggc ttgtggacga
    30361 gatagagctc gagcttttcc acggacagac acgggaggtg aagtcctcgc gcctcggaga
    30421 tatggtactc cagcggctgg aacagctcga caaagtagct cgcctccggt tcgcttcggt
    30481 cttcaaggat ttcgaaaccc cgggcgcctt tatcgacgaa cttaagctat tcggatgagt
    30541 ctccttcacg cggaggggtg gctgagcggt tgaaagcagc agtcttgaaa actgccgacc
    30601 gacgaggtcc gtgggttcga atcccacccc ctccgccagc actggttgcg aaggcccctg
    30661 cgagtttgcg tttcggcctt tttctccctc cttactcccc gcgccacaaa acgagaccta
    30721 cttcccggtt gaaacgcctc gattggagcg cagcttgatg atccgtatcg cagtggtagg
    30781 aatgggctac tggggcaaga acctggttcg ggtttttcac aagctcggag cgctccacac
    30841 ggtctgcgac accgtttcct ccaaaaagca ggaaatcgag cgcgagtacc ccggcgtttc
    30901 tttcacgtct gacatgaacc aggttctcgc ggatacgagc gttggcgcag tcgtgttcgc
    30961 aacacctgcg gcggaccatt ttcggcacgc catggcggct ttggatgcac acaaagacgt
    31021 gtacgtcgag aaacctctcg cgttgacggt cgccgatggc cgcacgatgg tggaagcagc
    31081 agcccgcacc tcgagaatcc tcatggtggg ccacatactt cgttaccacc ccgcgattcg
    31141 cacgctgggc acgatggtgg aggcgggaga actcggggaa atccgttatg tctattccaa
    31201 ccgcctcaat atcgggaaga tacgcaccga ggagaacatc ctctggagtt tcgcgcctca
    31261 tgatatctcc gtgatacttg cgctggtcgg gcgctaccct gaacgcgtct cgtgccacgg
    31321 ctcggcatat ctgaacaaag gtgtcccgga cgtgacgatg agcgatctgg aattcccgga
    31381 gggcgttcgc ggccatcttt tcgtcagctg gttgcacccg ttcaaggaac agcgattggt
    31441 tgtggtcggg tcgcggaaaa tggttgtgtt cgatgacact tcatccgaga aacttgtcgc
    31501 gtattcacac ggcgtggaat gggtgaacga tgttccaacc gccatcaaag cgcctcgaga
    31561 acacatcgac gttgcggatg aggagccgtt ggagaatgaa tgcaggcatt tcctgaaatg
    31621 catcgaagag cggtcaaatc cactgacgga cggtcgggaa gcgctcgcgg ttctcgaggt
    31681 tcttgaaacg tgccaggaat cgttggagag ggggggagta cctgtgactc tggtcgggac
    31741 tgcaaaaccc gaacgcgcgt ttttcgctca tcctactgcc atcgtggacg aaccctgctc
    31801 gatcggggcg ggaacgcgaa tctggcatta cagccacatc atgaagaatg cggtgatcgg
    31861 ggagaattgc gttttcgggc agaactgcaa cgtggacagc ggggttgtgg tagggaataa
    31921 cgtcaaggtt cagaacaaca cctcgcttta cacagggctc attgttgagg acaatgtgtt
    31981 ccttgggcct tcctgcgtgt tcacgaacgt gaccaacccg cggtcggaga tctcgcggcg
    32041 gggcctttac gagaagacga tcctccggag gggctgtaca atcggggcaa acgcaacgat
    32101 tctttgcggc acgacgatcg ggaggtacgc gtttgtgggc gcgggcgcag ttgtgacgaa
    32161 agacgtgccc gactacgggc ttgtggtcgg caatccggca agattccgcg gatggatcag
    32221 ccggcacggg ttgccgttgc gtacccccga tgcgaacggt gtgtacgtct gccctgaaag
    32281 cgggctgaga tacaaagaag ttgctccggg agtgatccgc tgcctcgatc ttgacgagga
    32341 agccccgatg ccggaggaga tgcgcgtggg tttgaaatcc tacgacgcca tagtacaccc
    32401 cgagcgatcc gcctagctcc taattacgcc gcgctctgct ttctgcggaa ttccgcaact
    32461 ttcactgcta cgtcagggtc gccggtagcg atgatctgcg ctgcgaggac gccagcgttt
    32521 tttgccccat taatcgccac acacgctaca gggatgccgg gcggcatctg gacgatcgaa
    32581 aggaggctgt cgaacccgtt caatggtcct gacgcgcagg gaacgccgat caccggcagg
    32641 cttgtatggg cggcgactac tcccggaagc gccgccgcca tgccggcacc cgcaataata
    32701 accttcacac cgcgatcctg agcttctcgc gctatctgcg cggtgcgatc cgggtttctg
    32761 tgcgccgaag agacgacgat ctcgctggaa agcccgaatt cctgtagctg tgcggcagcc
    32821 ttctccatca caggcatatc agaagaactg cccatgagga tgagcacgtc cgttttcccg
    32881 gtttcctttg cctcatttcc catcggtttg ccccgtcatt tcaaagttga actcgccagc
    32941 cgcgcaatgg cgcggtgtgc gatatccttc cggtagtgca tgccatcgaa atggatatgg
    33001 tccactgccc tgtacgcacg acctacggca gtccgaatgt cgtccgccac agcggtgacg
    33061 ccgagaacgc gtcccccggt ggtcaccagg gaaccatcct tccatgccgt gcccgaatgg
    33121 aaaaggatta ctccctcctg ttccgccgct ttttcgatac ctgaaatcct gaatcccttt
    33181 gtgtaatccc ccgggtaacc acctgaagca agcatgacta ccactgctgc tccttcgtct
    33241 tcaccgaccg agacggtatc cagtttcccg tcaatcgccg cttccatgag ttccatcatg
    33301 tcagagcgaa gtaacggtac gaccacctgc gtttcgggat cgccccagcg gctgttcatt
    33361 tccaccactt tcggcccttc ccgggtaatc atgagaccta cgtaaaggat accatggtag
    33421 ggacatccct ctgcggccag cgccttcaac gttggttcga tgatacgctc gcacgcatgt
    33481 tgcctgacct cgtcggttac caccggggcg ggtgcgtatg cgcccatccc gcccgtattt
    33541 ggtccccggt ctccatcaaa caccggtttg tggtcctgcg ccgaaacgaa caggcggtag
    33601 tttctgccat cacagatggc gaaaagagac gcttcttcgc cctccatgaa ctcttctatc
    33661 acaacttcgt tgcccgcatc gccgaacgtg cgtttcacca tgatctccca gaccgcttcc
    33721 tgcgcctcat cgcggcttga agtcacaatt gcacctttgc cggcggcgag gcctgaggcc
    33781 ttcacgacga cccgatgaga tacgcttgag acgtagtcac acgccttttc gggatcggaa
    33841 aaagtcttga acgcggcggt aggaaccccg gcttcccaca taagccgttt ggcgaacgcc
    33901 ttgttgcttt cgatgcgggc agccgctgcc gttggaccaa aggcgcgaat tccggccgcc
    33961 tgcaacctgt ctaccaaccc cagcgccagc ggatcgtccg gcgccacgag caccaggtcc
    34021 ggtttttcct tccctgcgag tgcaacctga ccgtcaatgt ccgtcggttt gacgtcgaag
    34081 cactccgcaa ttgacgctat gccggcgttt ccgggcgcag ccataaggcg gttcacaccg
    34141 ccctggcgct tcaacagcca cgcaagggcg tgctcacggc ctccctgacc gacaatcagg
    34201 acgtcctttt tcttcgtttc aaacatgctc atcctttcga gtgtactgtc cttccggccg
    34261 atgacccggc ctgatagctg tgagccgttc tggcttccct gtggagcggt tctagcgcca
    34321 ccagactcgt gccctaaggt ccgaaacccg ccgttccaga aaaacgcccg cgatcttccc
    34381 ccttagacgg agctggaggc atgcgtcacg atactccggt ccgaaccggt cgaggcactg
    34441 gatgcatctg tcgagggaac gtcttgcgcg caggcacaat gcgatgttgc cgcacagggt
    34501 ttcctcatcg tatcccagtt catggccagc gagaacgtaa tcggctacaa gaaaacactc
    34561 ccggcagaga atccgcgtga ccgattccag cggacttccg atcacgtttt cacaaatcgg
    34621 ggcaacgaat tccatcgtgc gatcgcagaa ctccacgact gcacgccagg caggcatctg
    34681 gcccaacccg gcgtacaatt cgcggatcaa ggcgtcttcc tcttcgagga gtgtgtctgc
    34741 agaagcggca gcaaggagat cgtccatcgt gcctgcaggt tcggaggatg gttgaacatc
    34801 attcgttggc tgttcgcggg acgtccgcga tctgagtgcc aggaagaacg attcatcctg
    34861 cctcgcaaag tagtcctccc actgctgttc cgaccacccg cgcgtgctct cctgcatgtg
    34921 ttccttacca ccttctgagc cttctgccga acccctcttc gcacggagga attcgaatgc
    34981 cggaatgaat tgaataccta atcgcgctcc agcggtcaac tcctcgaccg catgcgcccc
    35041 tgggaatggc gcgttttgac gcgtactttt tgtttgcatg tgccgcggcc tgatggcaga
    35101 acggaggtac cctgatggaa cacccgttgt tacgcagaat gctgcagaac ggaattgtcg
    35161 gtgtcgttcg aacgccgacg cctgagacgg cggcaagggc tgtggaggca gttgtctcag
    35221 gcggcataaa agttgttgag atcacattta ctgtgccaaa cgcgaccaaa gttctggaaa
    35281 cggtggccgc ttcgacgggc gatgacgtgg tgctgggagc cggcaccgtg accgattccg
    35341 agaaagcgaa agcagcaata cgcgccggcg cgcgattcat cgtggcaccg aacacggatc
    35401 ccgcaacaat cgccacggca cacaccctcg gagcgcttgc catacccggc gctcttacgc
    35461 cgacggaagt ggtgatggcg atgaacgcgg gtgctgatgc ggtcaaggtg ttcccgattt
    35521 ccgtgtttgg tccgtcgtac ctgaaagcgc ttcgtgcccc gctgcccgat gttgtgctta
    35581 tgccaaccgg tggcgtcgaa ccggacaatg tttttgacta cctcgcagcc ggagcacaac
    35641 tgcttggcgc cgggggaacg ctggtcgagt caaagcacat tgcaacgggc aattacgagg
    35701 caattgcacg aaaagcgcgg tctttcgtgg atgcgtacaa tgaagcgata gccagactcg
    35761 gctcgaaccg atcgtgaagc gggaaggaac ggtattccat gagcgaggtg gtaacattcg
    35821 gcgagggcat ggtacggctt tcgccaccac atttccagcg cctggagcaa acatcgacgc
    35881 tcgacatggc agtgggcgga gcggaattca atgtcgccac cggagtcacc cggctgggtc
    35941 tgacagcctc gtgggtttcc agattgcctc agaacgccct cgggcgggtg ctggtcaatc
    36001 aggcccgaca actcggagtt gatacctctc acgtggtgtg gtcacgcgaa gaccgcatgg
    36061 gaatctattt cctcgaattc ggatcggcac cacgtccttc caccgtgctg tacgacagga
    36121 agaattcggc tatgagccgg atccggccgg gcgaggtgga ttgggacagc gcgctggcgg
    36181 gagcaaagtt gttcgagact tcgggcatta cgcccgcact cagcccgagc gcggcagaag
    36241 cgaccgcaga agcgctcaga atcgcgaggc agaagggcct caagaccgtg tacgacccca
    36301 actatcgcag caccctctgg tccatcgagg acgcgcggaa agtgcagacg ccgctcatgc
    36361 gggacgtctc ctgtttcatc acgggagtgt cgatggaggg ggagacaaaa gaagctgttc
    36421 tgggagtcac gggttcgtcg ccccatgatc tcgcgattcg gcttgtggag acgttcggat
    36481 ttgaagtggt tgcgctgacg ttgcgtggaa cttccggact gatgcgcaac tcactgactg
    36541 ccgtcgcgta ctcaaacgga cggtcctatg aaacgaaaag ctacgatctg gaagtagtgg
    36601 accgggttgg cgggggtgat gcctttgccg cggggtttat tgcgggctac ctggaggatg
    36661 acatccagaa ggcactggac tatggcaccg ccttcgccgc gttgaaacac actgtccccg
    36721 gcgatatcaa ctggacgact ctccaggaga ccgaggacct catcaagggt gcggggttga
    36781 ggattcgccg ttaaccgatt ctctgacgca ccgctgaaag gacgcatgag actttccatt
    36841 gtaaccgacg aacttaaaac acccttggcg gaggcgctgt ctattctgga tgggtggaag
    36901 gtccggaata ttgagttgcg gggcctcgac ggcggcagga tccccgacgg tgatgttgag
    36961 aacgcgcttt cccgcgtccg tgcgcacggt tgtacggtga cgagcctttc gccgggagtc
    37021 ttcaaatgcg cctgcgatga gcaggagatc gagaagcaca ttgaaaggtt ccaaagaacg
    37081 ttggagctct gcgattcttt cgggtgcgga caggtgattg tgttcagcat tcaaggacag
    37141 gagtcccgtt tagccgcacc tccggccctt gttgttgagg cgctgcgcga gaccggccga
    37201 atcgctgccc agcatggagt gcgcgtcgca atcgaaaacg agcccgggta tgccgctacg
    37261 gggtgccgcg ctctggcgaa cctgatcaac gcagtgggca tggaaaacat cggcgcgaac
    37321 tgggacccgg gtaacgcatt cccctttgac cccgaaatcg acgacgccgt ccgtgtgctg
    37381 gcaggacgca tattcaacgt gcacgtgaag gacacttctg tacaaggtga tcaccgtcgc
    37441 tttgacagcg tcggaaaggg acagatcaac tggaagaagc agatcaacga cctggcggca
    37501 tcagcctacg agggctgtct cgtgatcgag acgcactgtg aaccggggat tgagaaaaca
    37561 cgcgaaagtc tggatgcggt tcgcagactc cttcaggacg tcacaccccg gattggaggc
    37621 tgaactggcc atggcgcaga tcaaaacgct cgaagatctg gagcggatgg ctcccgcgtg
    37681 tcagttggct tccgaaaccc ttgagtatct gaagcaggca gttcgcgccg gtatttccac
    37741 gctggaactc gacgcgctgg cggaaaaata cattgccgat cgcggtgcga ttgcaagtcc
    37801 gaaagggtac cgtgggtatc ccaacagcat ctgcactgcc gtcaatgatg tcgtggtcca
    37861 tggcatcccg acagcgaaac cgctggagga gggtgacctg atcaccattg atgtgacggt
    37921 ctacaagaac ggcttccatg gggacaaggc cgctaccgtc attgttccgg ggaaaaagaa
    37981 ccctgaaggg gagcgcctgt tgcgcgtgac agaagccgcg atgtacctgg gaattgccga
    38041 agcgcgcgct ggtgcacgtc tcggcgacat cggcgcggcg atccagcgcc atgcggaaag
    38101 cgccgga
//
LOCUS       DAOSOO010000009        36824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1169_length_36824_cov_6.612608, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000009 DAOSOO010000000
VERSION     DAOSOO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 36824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36824
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1169_length_36824_cov_6.612608"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLT86_01425"
     CDS             <1..237
                     /locus_tag="PLT86_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35306.1"
                     /translation="LIGVAIGISCFALGHWAIHGLVMGLVFSLPTGFGSMLGPEVQGF
                     TPWSMFMWTVILGGIYGVVIELVTTVVFKAKMRE"
     gene            242..1270
                     /locus_tag="PLT86_01430"
     CDS             242..1270
                     /locus_tag="PLT86_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35307.1"
                     /translation="MPIPVDWLLAGEPFVEYRTRRDLLDQPETDESVRAARNAMLADP
                     RVIALIAGLSEWPGTVIASHRSASQPFHRLNFLADLGLTVADDGVRAIANAVVEHQSD
                     EGPFQLPLDIPPQYGGPGQNRWAWALCDAPLLVYALAKIGMAADSRVLRAVNFLAGLV
                     RAYGWPCVVSKELGKFRGPGRKDDPCPFATLAMLKALSQFEHLRDGPEARTGVEALLS
                     LWVESRTQHPYMFFMGKDFRKLKAPLVWYDLLHVLDVLSQFPSAAADGRMRDMVDTLR
                     AKADSEGRFAPESVWQPWSGWEFGQKKAPSRWVTLLAWRILRRVGGESVARASDPGYA
                     VSPRWGCL"
     gene            1555..2007
                     /gene="tnpA"
                     /locus_tag="PLT86_01435"
     CDS             1555..2007
                     /gene="tnpA"
                     /locus_tag="PLT86_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOT35308.1"
                     /translation="MPQSLARILVHVVFSTQNREPVLNSGVRNGLFPYCAAVLNENGC
                     SALAVGGVEDHMHLLFALGRTATVCKVVEMVKTSSSKWVKRQGEELAAFYWQGGYGAF
                     SVSPTHAQTVVRYIARQEIHHRTRSFQDEFRGLLRRYSLDFEEERVWE"
     gene            2703..3782
                     /locus_tag="PLT86_01440"
     CDS             2703..3782
                     /locus_tag="PLT86_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35309.1"
                     /translation="MPAKAKSKPSKVRVGFVGSGGIARHYMGLFTKNMANDVEIVAIS
                     DSSDAALALAGDQFNIAKRFKNYKDMLKIKELDAVCVCTPNYLHAQPTIDALGAGKHV
                     IVEKPMAMNATEAAAMVKAGKDNKKILVIGFQYRYAPAAQVIKRAIDEGQFGKVLFAR
                     CQALRRRGIPNWGVFTRKDLQGGGPMIDIGVHIIEVAHYLMGSPKPVSAFGSTYTYIG
                     DKEEKAKGVACGWPNWDYKNYTVEDLAVGLIRFDNGATLSVEASFAAHIEKDVFSFTL
                     MGEKAGAQFEPLMLFKDEAGVMVNVTPTYVGGQDGFLTKLQDFIECVKTGKQPQAPGE
                     HGLMVQQILDGVYRSAETGKVVTFK"
     gene            complement(4305..4679)
                     /locus_tag="PLT86_01445"
     CDS             complement(4305..4679)
                     /locus_tag="PLT86_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT35310.1"
                     /translation="MKDVRTYLARIVECIDQIDAYTKGDRARFLQERIVQDAVIRNFE
                     VIGEAAKRVPEEFRARFPEIPWRMMAAFRDVLIHDYESVSMPRVWNVVQNELPKVRSS
                     LASVLPPLDELEKELSSDGSPE"
     gene            complement(4676..4969)
                     /locus_tag="PLT86_01450"
     CDS             complement(4676..4969)
                     /locus_tag="PLT86_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT35311.1"
                     /translation="MDEIERLLREKRHEILRIAARHRARNVRVFGSIARGDARPDSDI
                     DLLVEFAPESDLFDQIALKRDVEELLQRKVDVVGDDSIYWLLHRRILKEAKQL"
     gene            complement(5626..8748)
                     /locus_tag="PLT86_01455"
     CDS             complement(5626..8748)
                     /locus_tag="PLT86_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT35312.1"
                     /translation="MNSPENRFPPVQHTGPLAWMVHNRVTATLLMLVFIIGGLVTAYR
                     IKQEVYPEFELDRVDIRVPYPGASPEEVEQGIILVVEEAIRGIDGIKQITATAAEGSG
                     SVSVEIEEGADAQRVYQDIQQAIQRISTFPQDAEKPQVALAISRRNVLAILIYGDVDD
                     WVLRDVAELVREKLLQKPGITQVDLSGVRDYEIHVDISQENLRRYNLTLAQVAQTIRQ
                     VAVELPGGSIKSDAGEILLRVKERRDWAREFGDIPIITTPSGVVVHLSDIAEVREGFE
                     DSNRLATFDGQLAVNVDVYRVGDQTPIGVSNAAHEALEEIAPDFPPGVHYAFRMDQSV
                     TYRQRLDLLLRNAASGLILVLLVLGLFLAPKLALWVTMAIPTAFLGTILFMPALDVSI
                     NMMSMFAFIMALGLCVDSAIVAGENIYEYRRRGMEVLDAAVQGVREVNLAIAFSMLTN
                     IASFVPLLFMPGWIGKIWRVVPFVVIFTTLIAWIQPILILPAQLARSLAAPEGSAMHH
                     VAVFQQKVSGALYRFINNVYDPFVEKALRRRYLTIAIFVAVLTIVVAYAASGRMGIVL
                     MAKVESDRAYVTATLPYGSPFQRAVAVRDRLVESAEKIRAEYGDALVQGIYANINENV
                     VEVAIYLTDPDIRPLSTGETTDIWREKTGQIPGLQSLKFESDRGGPGGGSSVTVELTH
                     SNIDVLAAASRDLARKLAHFTQTRDIDDGYSPGKQQLSFRVRPEGLSLGLTPTDVARQ
                     VRNSFLGTEALRQQRGRNEITVRVRLPEPERLSEYDVEQLMIRTPAGRDVPLYEVAEV
                     ERGRAFTSIARRDGRRTVTVRASVIPEKETNRVLESLQMEVLPQLVRDFPGLSWSFQG
                     RQATMTESMDSLYRGFLLALLVIYVLLAIPFRSYSQPLIVMTAIPFGIVGALLGHLIM
                     GYSIDLISMMGMVALAGVVVNNALVMIDYANRCRLEGDNAHDAIHRAGIRRFRPIILS
                     SLTTFCGLVPMIFETSRQARFMIPMAISLGYGILFATAITLILVPCLYLVIEDVKGLG
                     KGREEQETAQ"
     gene            complement(8745..9986)
                     /locus_tag="PLT86_01460"
     CDS             complement(8745..9986)
                     /locus_tag="PLT86_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT35313.1"
                     /translation="MADSSTPTPIPQKGGRRILRAAVPLAIVIAGSLVAFYLMKTAPR
                     AERRQRPVNARLVEVDTLHFAEHVVTIEAMGTVQPAQEVQVAAPVAGEVLSISPELIP
                     GGRFRKGQLLVQIDPADYDFAVERAAAEVTRARSELATEMGKQAVARREFELLGQQGG
                     EAERALALRQPQLETARAALASAEAALAQARLNRRRTSVFAPFNAIVKVAYVNAGARV
                     SANSPLAALMGTDEYWVTASVPVNQLRWINVPQSATGKGSPAVISNDVEWGSGVVREG
                     YVARVAGDLEEQGRMARVLISVPDPLALTRNNEGKPSLLVGAYVRVTIRGNPVPSAAK
                     IRRDLLREGDRVWIMNDSSRLEIRPVTIALKGPDVVYVAGGVREGERIVTTDLSVAVE
                     GQPLRTREEPAARNATRGTSR"
     gene            complement(10017..11429)
                     /locus_tag="PLT86_01465"
     CDS             complement(10017..11429)
                     /locus_tag="PLT86_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT35314.1"
                     /translation="MTGCGAKNSVIAYLVLPAALIAGCGPKPAGIAPPVAPPAEFSAA
                     GTQEAADRWWQSFGDAALNEAVERSLADNLSLRGAWFRLAQAEAAARREGAGAYPSMS
                     LSAGAELTRQHTGNVATNGTDTEFSLGAAASYEVDLWGRVRAASDASLLDARASAEDV
                     RTAAVTLSAQVATTWFRISGQEAETAVLRRQLRSNEEMLELVTLRFQQGQSKAEDVLR
                     QRQAVEARRGDIARGEANRAVLCNRLAVLLGQNPGTSAFRPPPDLPDLPPMPAAGVPA
                     DLIRRRPDVHKAYFEVMAADRRAAAAIADRFPRITLSAGANTSAESVTDLFSNWLASL
                     AANVAMPLVDGGRRKAEVERTRAATSASLTAYGQTVLDALAEVENAIATERRQQEYVT
                     SLRQQLSLAAEVINRARDNYLQGVTEYTRVLEAQQTHQALEISYLGAQLALIENRIAL
                     YRALSGGWELTRPGPLTGSR"
     gene            complement(11459..11902)
                     /locus_tag="PLT86_01470"
     CDS             complement(11459..11902)
                     /locus_tag="PLT86_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT35315.1"
                     /translation="MARTVLVVYHSMGGNTAAAAEIVAEGIRSVAGADAVVKTAAEAG
                     PSDLLACDGVCFGSPDYFSYMAGMLKDFFDRTFYPTQGQVDDKPCAIFITHGGGGSAS
                     TSVEKICRSFRFKQVGQTLLVRGKPDDAARTKLRDLGALLAKTVL"
     gene            complement(12000..12179)
                     /locus_tag="PLT86_01475"
     CDS             complement(12000..12179)
                     /locus_tag="PLT86_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35316.1"
                     /translation="MPQTTEGGLRKGKRHIKTTPPGSDVEDAGFSAGLLKQGPGKPLW
                     ILSPLKGPMRTAAKW"
     gene            12184..>13751
                     /locus_tag="PLT86_01480"
     CDS             12184..>13751
                     /locus_tag="PLT86_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35317.1"
                     /translation="METPFRVLEIGTTPQLLIDDYVVEDVWMVRRSPEEPVKYLGNPI
                     FPAGDTVIYDEEEKLFKMWYGIGIPGTFEAKGAYAVSSDGLHWEKPELGLVEADGSTR
                     NNLIETTGFGSVLKDPHDPDPARRYKMMTKRAGTPESEGRAFAAFSPDGIHWKDHPGE
                     KSILRASNDGNGMVLRDDSTGKYINFRRTTVLAGGRGVLPEDIGFPDPRVMKGAGGDK
                     LGVPEGIGFPSEDDFEAYEEAEEYIHRYLRPAPYVHTRTLRIFRDTHEGLGCNRRIAR
                     SESDDFLHWTEPEVIIRPDELDPPRLYSISVARYLGMYIGLLELYDSCGNRRAPGTPQ
                     ESDTIDTQLVFSRDGWKWERLANRPVFLRRGIIGAFDCGMLRPVNPPFVVLGDEIRIY
                     YYGHGSSHNIPSPNQGLGVARLPKERFVARVAGDEVGALITKPFRLEGGELQINANAA
                     KGLIKVEVTDPFGNPLEGFSAKEAVEIRENGFRVPVQWTCGKRLSEIAGRVVRLRFYL
                     YRSRLYSFVVDEGR"
     assembly_gap    13752..13851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13852..14329)
                     /locus_tag="PLT86_01485"
     CDS             complement(<13852..14329)
                     /locus_tag="PLT86_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT35318.1"
                     /translation="MCKPFVDLSVEEIAHTLDVSLWGVIHTAQAAARLLVRQNTGGAM
                     VFVSSVHVPFPYANCADYNIAKAGGHALAMTLAAELAKHRIRVNIVEPGWVDTPGERK
                     WSSEEDIAREGAKLPWGRLATIEEIGQAVAFLASEKAEYITGSVLKVDGGFTLPRAG"
     gene            complement(14310..14609)
                     /locus_tag="PLT86_01490"
     CDS             complement(14310..14609)
                     /locus_tag="PLT86_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT35319.1"
                     /translation="MEKGEKVALVTGASRGIGRGCAEALAADGWSVVVNYRSHEDEAR
                     AVAERCRGFGVRAVTAMADVRDREAVDAMFARTVEHFGGLHLFVSNAADSVQTVC"
     gene            complement(14703..14900)
                     /gene="rpmB"
                     /locus_tag="PLT86_01495"
     CDS             complement(14703..14900)
                     /gene="rpmB"
                     /locus_tag="PLT86_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT35320.1"
                     /translation="MAYQCELCGKSFQRGNTVSHANNRRKRLFKANLQRIHVMVGSTS
                     KHVRVCTQCIKSGRVEKKTAA"
     gene            15094..15390
                     /locus_tag="PLT86_01500"
     CDS             15094..15390
                     /locus_tag="PLT86_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35321.1"
                     /translation="MHTWGVAVLLMVVTWVVLAGLPGAPVQSLAGKLILAIGFFVIGV
                     GFYLLSIGPVDSVQSMTWAPLALVAGFCVIVPLGLLYHRRAPGSEEDAATKTRL"
     gene            15396..15469
                     /locus_tag="PLT86_01505"
     tRNA            15396..15469
                     /locus_tag="PLT86_01505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15430..15432,aa:Val,seq:tac)
     gene            complement(15522..15992)
                     /locus_tag="PLT86_01510"
     CDS             complement(15522..15992)
                     /locus_tag="PLT86_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35322.1"
                     /translation="MKKPQIPLSENHRRIVGSSLRYAEDQILFCRRLLTETSENRMTR
                     FADEYSDDQIRRLVEMCDELHEKLVSFADRAGVSPAERSVSRHVSGTMSSVWETLEDL
                     RPRKLKAYGEVPADAAREIEPIVEDAVQIIERVLEFSRRCTSRSDSGAGARQPE"
     gene            complement(15989..17830)
                     /locus_tag="PLT86_01515"
     CDS             complement(15989..17830)
                     /locus_tag="PLT86_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOT35323.1"
                     /translation="MCIPETDVRAPQTAGGENGDITNANGLGELFDEALSILGTAGPE
                     FKTFSDAIAELKTRLAEERFHLAVLGQFKRGKSTLLNALLGEAILPSSVVPLTAIPTF
                     VRAGETRRALVYFDNGVPPRAFEADDAGAIREFIAQFVTESGNPKNERGVTQVDVFSS
                     APILRQGVVLIDTPGIGSTHRHNTEATLNFLPQCDAAMIVVSADPPITEVEVEFVRQV
                     KRRINRLFVVLNKIDYHTEADVREATGFLKSVLADKLGFSDDHPIFPVSAQLGLNARQ
                     NADADLWERSGMHRVERHLSRFLASEKKAALSEAVRHKAADTVEEALTQFRLILRSLQ
                     LPAEELDKKRAFFRQKLDEIERNRLVADDLLKGDHRRTRARLEEYIDAVRAQARTGFR
                     GIVAGALARNADTQQAESDARDALAEAIPVFFEQKVGQTSAFLREEIDAVLTPHQSRA
                     EELVESVRKAAASIFEVPYQPAGKVITFDIVQKPYWIARTWSTSLSPIPEGFWDRFLP
                     ERVRRHRLQTRLNNQLEDLVISNAENLRWAMLRTVDETFRHFRSTLVTRLGETITATN
                     GALNSATEKRGLHEEQTASLIHGIQETIRQLENVEHSLRPPPTGAAP"
     regulatory      complement(17853..17917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(17986..19203)
                     /locus_tag="PLT86_01520"
     CDS             complement(17986..19203)
                     /locus_tag="PLT86_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT35324.1"
                     /translation="MAAIRNAIDFLGLKRSIIGVLSMVVLVGMGERMAERFLPIYLFA
                     LGGGVLSVGLLNGLDNLLSALYSFPGGYLADRIGTKRALIVFNLMAMTGYCVVIFIPV
                     WQAVIIGAFFFLSWTAISLPATMGLVAVVLPKNKRTMGVSMHSLVRRIPMALGPLVGG
                     AFISIWGEREGVRAAFIAAFIFAAVAMVMQQLLIEDKREEASAPLSGNPFRLIGLMNA
                     DLRNLLVSDILVRFCEQIPYAFVVIWCMKVISNPVSAIEFGVLTTIEMATAVLCYIPV
                     AYFADKSGKKPFVVITFVNFTLFPVALLFSHSLLPLVGAFILRGLKEFGEPTRKALIM
                     DLAPEGAKAGMFGLYYLLRDSVVSLAAFGGAFLWMISPAVNLISASAFGLAGTLWFLW
                     RGRDMPVLSTATA"
     gene            complement(19360..20586)
                     /locus_tag="PLT86_01525"
     CDS             complement(19360..20586)
                     /locus_tag="PLT86_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT35325.1"
                     /translation="METLFTHAAVWLFLAVISAVLANHLKVSIALMEICMGVAAAAII
                     THFWGANSFPNNEEWLKFLASSGAVLLTFLAGAELEPEVMKTKLKEVSIIGFVGFLAP
                     FVGCALVAHYGLGWDLRASLLAGIALSTTSMAVVYAVMLETGFNRTEFGKGILGACFV
                     NDLGTVLALGLIFAPFTYRTFVFIGGCLVVFFILPYATSFLTRHYGHRTAAIRTKWIV
                     FVLFALGALALWSGSEAVLPAYIAGMVLAEFTSDDAHFMRRLRTLTVGFLTPFYFLRA
                     GSLVALPALIAAPAVFIVFLAAKTVSKIFGLLPVVSAFRTEKNERWYYTLMMSTGLTF
                     GTISALYGFSHGIVTQSQYSFLVATVIASAVIPTLIANAAFMPRHLLPETATVSVEPA
                     GRGALPPKEEEDVGEE"
     regulatory      complement(20666..20735)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            21124..25134
                     /gene="porU"
                     /locus_tag="PLT86_01530"
     CDS             21124..25134
                     /gene="porU"
                     /locus_tag="PLT86_01530"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOT35326.1"
                     /translation="MLVGAALSVCAAGSAPARWELIQADASGVVAEWSSPVVRMDTVV
                     VAGQRTLQPVLEDCSADGEPGSPAIPKAGTLIAVPDGAQASASAVAGEWETRPGLLSP
                     VPSHELREDGLGYRRVFEIRPEAYSAQYTGTDLVSLEPAGEAYGAHLVRIVVHPVRAN
                     LADKSLAITRSVRVRVQFTSPETGSLRDITPLPSVWEGAVLNAAAYNALQRTSRSAAD
                     RVARVAETSPFASGVWLRVVVDADAMYKITAAALAEADSRFRSAPAERLALYAGSGRE
                     LPLDPQKPRQTALRPVRPIVVDANGDGVFNGTDYLLFYGRSPSGWDLDYQTLDPVYAL
                     NHYTYENVYWLTISPATAIRAEVRNGAVSDPSLPVIERFPFRFHEEPEVTNLNEEGDT
                     DGPYSGVDWQWDQLAPQASRVLQVALLDVAGDTVGVRVGQLRQFSAYGSLQVKVNGVV
                     EPLVEQRQTSTYAWCTGYAPVITPAAQVSLPVEIRNASTGYPIILDYYEIAYWRRFVV
                     RGSSFAFVLPPLRNTIPSTRVVVPLSGVDRTLHRLFEVSDDSGLVEYALPPPDDQGIT
                     RVELRQSGLIERKYLLAAESDWRTPLRMEPKTGATNLRGRTAGVDYVVITHPDFQAEA
                     SRLADLRAQRNGFRTAVVTTRDICDEFSFGMFDPAALRDFLRHAFMNWRDGAADQGLR
                     YTVLLGDGQFDYRNLTRFTTSTANPQPGNWVPPYQEGPVTTDDWFVVFDNTGIASVKI
                     GRLCVQTKEEARAVVGKIIAYERDPERGAWQNRAIMIADDEYDLDRGINSEDYVDDSE
                     RMAGCLRAETVTEKIYSHERQLNQQMQKPSVNRMITDAWSRGAALINYVGHGNPVLWA
                     HERIFTLDGDLPNIANGKRLPVLTALTCSAAHFDAPSTQSMAEVLVNMANGGAVATVA
                     ATRLSYNTENVGFNEIFLSRLYGGAEGRTPRIGDAFWYAKALAAQNPSWLYRQNARKY
                     VLIGDPALTVALPELVVQVQMMGDSLRALAASTLRGSVLVPGATDTLRSFSGSALVNV
                     FDSATPMSYQAAGGNTILYTKPGTSIFRGVVPVQNGVFATELILPIEVSYGGTQAKAV
                     AQVWNDQIDGAGSIGGLGICSRPAASVRDTTGPSIVFTRADDLDAAIPLRDGAEIPRG
                     EPVRVWLSDPLGVNVGGGIGHRLVAYLNGNRSAPVDLTEDFVHWGSATTGWADIPLSG
                     DLTAQQVVVEAWDNGNNFAADSVFLRVGRGKDIAVSNVIAYPNPMARDGAFTFVLDGV
                     GASTDADATVRVFTVAGRLVDTVTKLGVTDGPVVIPWTPAYQLANGVYLYQISIRRRS
                     DGRTAKALERLAVVGP"
     gene            25156..26322
                     /locus_tag="PLT86_01535"
     CDS             25156..26322
                     /locus_tag="PLT86_01535"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT35327.1"
                     /translation="MKVARFSHFTALVIAGASAAVAFLPSVSRANESQAAVLALLFRP
                     GARELALGGAGVAGARGSAASYYNPALLSWQAIAEGQRYPRAVGTTYYKILQNFGLND
                     MYYMYFPTMFSVKDWGQFSVNVTYLNLGEQQRTSEGGENLGTFNTYTLAVGLSYASKI
                     GVHTSAGITAKWFYDHLADAGTGFEKGDPTGTGFAVDAGIAYRPSDRLYFGAALRNYG
                     PNVQYIDAKQASPTPVNFTVGTSWKAFDTQYNDVTIMADLYKPLVQDYRKSWYLSILR
                     SWADESVYKVEEIPDGQGGVTRVEHRNTLREETRQVDVLTGVEYTYADYVAIRGGYFR
                     DWDGQRNWMTFGGGLRLPIASTALLVDFAYVHSLNGKGSDPNDGQQVYSIGFTF"
     gene            26454..29177
                     /locus_tag="PLT86_01540"
     CDS             26454..29177
                     /locus_tag="PLT86_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35328.1"
                     /translation="MHHRGLLFAFFALSSFLAAASARAVETIHLLALRVEFPKEDPDD
                     FTTGGNGLFDMRSGAEALATAGEAGFRYPFDLPPHDGRFLENHLTALRNYLETASQGR
                     TTITWEVFPPHPLPAYKAPQTLASYGSGGSVAEQTRNWVRFLKDALAVSQNDVGSLGR
                     FQSFLVFHASVSLQGTLSAELPALVLTSQEIASSGIPIPPEVQTAWFIPQQIQQPGGV
                     IGLNGAFAKTFLASRGLPVLSNTRTGGAAVGAWTLMDVGSDNFIQRPRTGSSSDTIRV
                     LGFVPCLPSAWEQMRLGWLAPETVRSDTTLLLAGLAAETSLPRALKVPITEDEYLLLE
                     LRHSAVETDRYHPAIEYSDGDTSGVWLKPENGLYDAYVPGSGVIVYHVNEARIRQWEP
                     TNSINAQPDRPGIMVVEADGYRDIGVTNLLGHPRANEGIGSRNDPFPVTGERILYPDG
                     EPAPGHPAVITANGSPGGVAISFSPAGTARDTVAVTVRWRTADTPARKGRFIGSAVVG
                     GVAVRRVPLDGVERNLIAVAGSDWNLWAFTDSLEPVGNANGLIGRFWGDVTRKPVIEA
                     DGSVLVVGLTTNTRYRYANGAWDGGTPVVQTPPVPSTMTCHLLRDVRKSDITVTGSGT
                     ITADSYARFGYLPEDPPPPRVVWSATTDSLIAPLSLGDVDRDGFPDILAVSRRSVDVF
                     SRSGTRTITYTLGRADSTEEFAGSALSTGAGNTIVAGKKGLSVFSGLMRRHPAATIPL
                     PAAATGSPALYAVRDEGIHAVVGTADGWLLDIPVSQGDSVIWGQMWGDGASSFALDET
                     GLVMPAAPAEDLMPGDRVFFYPSPVGKEEGMLRFFLSAQADVSVRIFTSMGEQVWETR
                     VPAGETVANAENEIRWPGGTRFATGLYVARITARSADGRQGGATIPVGIVR"
     gene            29399..32305
                     /locus_tag="PLT86_01545"
     CDS             29399..32305
                     /locus_tag="PLT86_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35329.1"
                     /translation="MAAEQAYEPVTRLYGYRPSGKTEIIVRDHGDFANGFAAYYDNRM
                     EIWASALDYEFRGTHNWICDVTTHEFTHLVQLGASLKTSPAIPQVYFQWFRLEPEART
                     DVAEGLPNTLVSYAVPTVSIPMWFAEGVAQFQAQGARHDRWDAHRDMIVRVAVLADSQ
                     LSYRQMEGFYDPDGREAEMVYNHGYSFVRWLAATYGDSSLRALSRDMRAWHRWDFGSA
                     LKQLTGKPGPRVYEEWLTQLRAGYEAFVAERKPQPAGTLLERPASRPGAESGDRPPVI
                     TPGGVPAAYAPPPAHSARASYYHAAPVWSPDGALLAYVSTDGQDYRLGSVWVRKPGTG
                     ELTVAPGSFRASSTVSWFPNGRTVVFSRAQRDDRNEWRYNDLVKADLQTGTVTKLTDQ
                     WRASYPAVSPDGATVAFVRNARGSTNLMLANADGSNVRPLTRWDDGTQVFSPKWSPDG
                     TRLAVSLARAGSRHIVVYRVVRASGGEVLETERVIASSDDDRDPAFSRDGTRVVFSSA
                     TDGVFNLYELDLERGSCARLTNVLGGAFHPDVAPDGRIAYSSYESAGYVIRIIPADAP
                     RYPVSADIFANRIPEQGTFPDSMPEGSSVSDIDRRIPHLQRPSILPRFGRYGNQWRVG
                     AYAFSSDIWDDVLLMGGVWAAPENRDYDAFVTAEVDHMTPVPVLFDVVRTVRHTKEDT
                     TFAGRLRLDGITYGLNAVSLTLKPRVFSREIDLHSTYQRFDAAIDQTLLLNGTRTYVG
                     YNYTYYNGFAAGASVSSSAIRPFTTADIAPQGMKWSLRYDRWWNWYFEDFDAGTGLLN
                     EVYTPYHYNQVSCDAAWYLPTPWREEHTVGVEGRVMRIDAAEDSFFYEGIGGIIGLRG
                     YTYYQLQGKQTAWGRLLYRFPLLTSIDRKLGPVYFDKIYGTVFAEAGRVWRDSYPSSW
                     TPGLKRDVGAEIRADLFSFYGYPSRFSFSAARALDAAPHSDRTKFYLTVLFGYL"
     gene            32637..35471
                     /locus_tag="PLT86_01550"
     CDS             32637..35471
                     /locus_tag="PLT86_01550"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOT35330.1"
                     /translation="MTEHGNGPESGLEIDPSLLDADAVIRALHTDPEHGLSSAEAAAR
                     LARYGKNELVGAPPVPVWRKLLAQFEDPLIYLLLAAVAISAVAWFMEGAHGWPVDSAV
                     ILAIVVANGVLGYVQEAKAENAVAALASMAAATSAVVRNGQVERIPSSDIVPGDVLVL
                     SEGDNIGADGRLFKTANLRVAEAPLTGESEPVQKETSALQEPSNLGDRRNMVFKGTAV
                     TQGVGRAVVTATGMNTEVGKIARMLETTEDEATPLQKEIVRLGKMLGIAVVLIAAVVV
                     TTTFVVFRVDTMDEAVSLLLLGVSLAVAAVPEGLPAVLSVVLAIGVQRMAARNAIVKK
                     LSSAETLGSASVICSDKTGTLTKSEMTVTKIVTASGEVELTGVGYDPSGRAEVAGQEI
                     GDSALRTEAQCVLAGGSLAGNASLRQRDGVWEIQGDPTEAAFLVAAQKLGGTQQIMER
                     YERLGEIPFTSERKRMSVLLRERNTGRLTLFSKGAPDVLLERCTHILVGNEVAPLDDA
                     RQAASVGNIEALSAQAFRTIAVAYRNLEGEGVIPAEEGAPVDESLEADLVYVGIVGIL
                     DPPRPEAAKAVVESHRAGVRVIMITGDHPKTATRIASDLGIVGKDATALTGMEIDKLD
                     EAALREAVRTTSVYARVAPEHKLQIVDALQAGGNVVAMTGDGVNDAPALKRAEIGVAM
                     GVTGTDVTKEAGNMVLADDNFATIVAAVHEGRVIFDNIRKFLRYLLSSNMGEVLAVFF
                     GVVLSTVIGLTSGDGGIVLPLLATQLLWINLVTDAAPAMALGVDPETEDVMARPPRKL
                     TDRVIDGRMWTTVLIGGSVMAAMTLLTLDILLPGGLFEGTGSLDTARTAAFTVLVFTQ
                     LFNVFNSRSEHASAFHRLCANRWLLGAVLLGVVLQVAVVHIPFMNTAFGTTPLTGEQW
                     ILCVVMASAVLWVEEARKLVWRTTNLKGKK"
     gene            35468..>36824
                     /locus_tag="PLT86_01555"
     CDS             35468..>36824
                     /locus_tag="PLT86_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT35331.1"
                     /translation="MTLPERFPPITAKCPHILHGGDYNPDQWMHTPEVWDEDMRLMRL
                     AGCNVMSVGIFSWAALEPREGHFEFGWLDTIMDKLAANGVYVVLATPSGSKPPWLSEA
                     YPEVCRVNAQGQREPHRERHNHCRTSPVYREKCRIINTQLATRYKDHPALVVWHVSNE
                     YNGGDCHCPLCYEAFHTWLKKRYGTLEELNHAWWTGFWSHAFTDWAQIRPVDPSIHGL
                     MLDWQRFKTDHTIDFFRNEIAPLREFTPDIPVTTNFMGFSPTLDYAKFARVVDVVSWD
                     SYPAYHDRPNDWLNAVEVSLTHDLNRSFKNKPFMLMECSPSVQNWKPVNKLKRPGLHR
                     VEALQCIAHGGDTVQYFQWRKGRGGCEKFHGAVVDHYPTEHTRVFREVADLGGGLASL
                     DDVVGTITPAEVAILYDWENRWAIDQTAGPRNERKDYQPTCVAHYRPFWSAGVSCDVV
                     NE"
BASE COUNT         7054 a        10993 c        11467 g         7210 t
ORIGIN      
        1 ctgatcgggg ttgccatcgg aatcagttgt ttcgcgctgg gccactgggc gattcacggt
       61 cttgtgatgg ggctggtgtt cagcctgcca accggattcg ggagcatgct ggggccggag
      121 gtgcaggggt tcacgccgtg gagcatgttc atgtggacgg tgatcctggg cgggatctac
      181 ggagttgtga tcgaactcgt cacgacagtg gtgttcaagg cgaagatgcg ggagtaagca
      241 tgtgccgatt cccgtagact ggctccttgc gggcgagccg ttcgtcgagt accgcacgcg
      301 gcgcgacctt ctggaccaac cggaaacgga tgagtcggtc cgtgccgcgc gcaacgcgat
      361 gctggccgac ccgcgcgtga tcgctctgat cgcgggactc tcggagtggc cgggaaccgt
      421 cattgccagc cacaggagcg ccagccagcc cttccaccgg ctcaatttcc tcgcggacct
      481 tgggcttacc gttgctgacg atggtgtgag ggcgatcgcg aacgcggtgg tggagcacca
      541 gtccgacgaa ggcccgtttc agctccctct ggacattccg ccgcagtacg ggggacccgg
      601 ccagaacagg tgggcctggg cgctttgcga cgcgcccttg ctcgtgtacg cgcttgcgaa
      661 gatcggcatg gcggccgatt cgcgtgttct gcgggcggtc aacttccttg caggattggt
      721 cagggcatac ggatggccgt gcgtggtctc caaggaactg ggcaagttcc gcggcccagg
      781 gcgcaaggac gacccctgtc ccttcgccac actggccatg ctcaaagccc tttcgcagtt
      841 cgagcatctg cgggacggcc cggaagcccg cacgggggtg gaagcccttc tgagcctgtg
      901 ggtggagagc cgaacacagc acccgtacat gttcttcatg ggcaaggact tccggaaact
      961 caaggcgcca cttgtctggt acgacctgct gcacgtgctg gacgttctct cgcagttccc
     1021 gtcggccgcc gcagatggtc gcatgcgcga catggtggat acgttgcgcg caaaagcgga
     1081 ttcggagggc cggtttgcgc cggagtccgt ctggcagccc tggtcgggat gggagttcgg
     1141 gcagaagaaa gcgccctctc gctgggtaac gcttctggcg tggcggatat tgcgccgggt
     1201 gggcggcgag tccgtcgccc gggcctccga cccaggctat gctgtctcgc cccgttgggg
     1261 ctgcctatga ccgctcagga agggcactcg cagggcaaga accacggcaa ggcctgaagg
     1321 gccgacatgg ctcagcccgc gggcaacgcc ccgggcgggg aaggccccgc ctgtgttacg
     1381 gcgttgagtt caaggcgggg cgcggagaag atcggaatta cgtcgcccgg gcctgcaacc
     1441 cgggctgtgc tgtttcgccc cgttggcgct gcctgcgcgt ttcttgaccg cgcaggatgg
     1501 ttcctatcag ggcgtggttc gcctgtagca tctatcggta cgcgaggtat tgccatgccc
     1561 cagtctttgg cacgtatcct agttcacgtc gtgttcagca cccagaaccg ggagccggtt
     1621 ctgaactcgg gcgtccgcaa cgggttgttt ccgtattgcg cggcggtcct caacgaaaat
     1681 ggctgctccg ctctggcggt cggcggcgtg gaagaccaca tgcacctgtt gttcgctctg
     1741 gggcgcaccg cgacggtttg caaagtagtc gaaatggtca agaccagttc gtcaaaatgg
     1801 gtgaaaaggc agggggagga acttgcggct ttctactggc aaggcggata cggcgcattc
     1861 tcagtgagtc cgacacacgc gcaaacggtt gtccggtaca tcgcgcggca agagatccac
     1921 caccgcacgc ggagtttcca agatgaattt cgtggcctct tacggcggta ctcgttggac
     1981 tttgaagaag agcgcgtgtg ggaatgatcc cgccgtccgg cccggcaagg cctgaagggc
     2041 cgccatacgt tagcccgcgg gcagcgcccc gggcggggaa ggcctcgccc gtgctgcggc
     2101 cttgagattg ccggcgaatc gcggagaaga tctgatttac gccgcccggg cctccggacc
     2161 cgggctatgc tgtttcgccc cgttggggct gcctgcgcgt ttcttgaccg ctcaggatgg
     2221 gtcctctcag cgcaagcgcc atggtgaggc ctgaagggcc gacatacgcc agcccgcggg
     2281 caacgccccg ggcggggaag gccccgcctg tgttacggcc ttgaggttca ggcggggcgc
     2341 ggagaagatc tgatttacgc cgcccgggcc tccgacccgg gctgtgctgt ttcgccccgt
     2401 tggggctgcc tgcgcgtttc ttgaccgcgc aggcggcatg ttacagcacc aagcgcgacg
     2461 cctgaacgtg tggaaacgcg cgcgaggctc cccgattcag cgaggccggg gcttcgacaa
     2521 gctcagccac cggcgcgctg ccgggcggct tgtcgtcgca actcgaaaca cggtccgcct
     2581 cccccctcgg cttttcccca cgggaaaccc ttgtgtatcc ttattcgagc atttgcgcgt
     2641 ctttttccgc ccgtttttcg ggctgacatc atcctttcac tactgcttac caaggagcca
     2701 tcatgccagc gaaggccaaa agcaaaccca gcaaggtgcg tgtcggattt gtcggttccg
     2761 gcggcatcgc ccgtcactac atgggcctgt tcacgaagaa catggcgaat gacgtcgaga
     2821 tagtcgccat ctccgattcg agcgacgccg cgcttgcgct cgcgggcgac cagttcaaca
     2881 tcgccaagcg gttcaagaac tacaaagaca tgctcaaaat caaagagctg gacgcggtgt
     2941 gcgtctgcac cccgaactac ctgcacgcgc agccgacgat tgacgcgctg ggggcgggga
     3001 agcacgtcat tgtggaaaaa ccgatggcga tgaacgccac cgaggccgcc gccatggtga
     3061 aggccggcaa agataacaaa aagattcttg tgatcgggtt ccagtaccgc tacgcaccgg
     3121 ccgcgcaggt gatcaaacgc gccatcgacg aggggcagtt cggcaaggtg ctgttcgcgc
     3181 ggtgtcaggc gctgcgccgc cgagggatcc ccaactgggg cgtgttcacc cggaaagacc
     3241 ttcagggcgg cggtcccatg attgatatcg gcgtccatat catcgaggtc gcgcactacc
     3301 tcatgggaag cccgaaaccc gtatcggcct tcggctcgac gtacacctac atcggcgaca
     3361 aagaagagaa ggccaagggc gttgcctgcg ggtggcccaa ctgggattac aagaactaca
     3421 cggtggaaga cctcgccgtg gggctgattc gtttcgacaa cggcgcgact ctttccgtgg
     3481 aggcctcctt cgcggcgcat attgagaagg atgtgttcag cttcaccctg atgggcgaga
     3541 aggctggggc gcaattcgag cctctgatgc tgttcaagga cgaggccggc gtgatggtga
     3601 acgtcacccc cacgtatgtg ggtggacagg atggcttcct caccaagctg caggatttca
     3661 ttgagtgcgt gaaaaccggg aagcagccgc aggcaccggg cgagcacggg ctcatggtgc
     3721 agcagattct cgacggggtg taccgctccg cagaaacggg gaaggttgtg acgttcaagt
     3781 agcgcgcgcg tcacagcgcg agtgcgagca ggaaaccgca cgtgccgtca tggtacgtgc
     3841 ggttttgcgt tggaaacaga tgcgccgctt ggaaaacgaa ccaggaagag ggatgagcaa
     3901 ccgtcattcc cgcgaaagcg ggaatccaat acactcgccg gtgcgtctcg atacaacgaa
     3961 cgcacggcgt tcgccactcg acgaccggcg ggctctggga tcgagcaggt tcgcgacagg
     4021 aaggaatgcc tgccccgcca tcatttcctc gtcgtctggt ggggcggact gcccataccg
     4081 gggcatgcca ccgccgtccg gccctgcgaa ccgggctgga ttattgcgcg ccgttggcgc
     4141 ttcttgccaa cgactattcc gcgggtttcc catcgttgtg gctgcctccc aggaaaaagg
     4201 cgacattccc gcgcaggggg caatccaacg caacgctgtg gaggcctcgg cgagaccgcc
     4261 ctgcccgcgg gtcgggaaca cacggggcgc ttgtccagcc tgccttactc cggcgacccg
     4321 tcgctggaga gttccttttc cagctcgtcc aaaggcggca ggacgctggc aagggaagac
     4381 ctcactttcg gcagctcgtt ctgtactacg ttccagactc gtggcatgct caccgattcg
     4441 taatcatgta tcagaacgtc gcgaaaggcc gccatcatgc gccacggaat ttcagggaac
     4501 cgggcgcgga actcctccgg aacgcgcttc gcagcttcgc caatgacctc gaaattgcga
     4561 atgacagcat cctgcacaat tcgctcttga aggaaacgcg ctctgtcccc cttcgtgtac
     4621 gcatctatct ggtcgatgca ttccacgatt ctcgccagat atgtccgcac atccttcaca
     4681 actgcttcgc ctctttgagt atgcgtcggt ggagcagcca gtagatactg tcatccccga
     4741 ccacgtccac tttccgctga agcaattctt ccacatcccg tttcaaggcg atctgatcga
     4801 acaggtcgct ttcaggtgca aattccacta gcaggtcgat gtcgctgtcg ggccgggcgt
     4861 ctccacgcgc aattgaaccg aacacgcgaa catttcgtgc gcgatgcctc gcggcgatcc
     4921 gcaggatctc atgccgtttt tcgcgaagta gccgttcgat ctcgtccatg attcgggcct
     4981 ttgataagtt tcatcaaata tactaaacgg cacagcacct gccttcggtt tcggaggcgg
     5041 cacgggtaag acttgaaaac gccacggctt ttgccgcatc ctgcgaggaa gcgctgcctg
     5101 cgcccgcaga cgagcgcaac accgagacaa ttcgggagct tgcgcccagc gcgcggttct
     5161 ggcgtgacgg gcgggaaccg accgttgtat cgagaggccg cgccggtgcg tctcgataca
     5221 gcgaacgcac agcgttcgcc actcgacgac cggcgggctc tgggatcgag caggttcgcg
     5281 acaggcagga atgcctgtcc caccatcatt tcctcgtcgt ttggtggtgc ggactgccca
     5341 taccggggca tgccaccgcc gcccggtcct ccgaaccggg ctggattatt gcgcgccgtt
     5401 ggcgcttctt gccaacgact attccgcggg tttcccatcg ttgtggctgc ctcccaggaa
     5461 aaaggcgaca ttcccgcgca gggggcaatc caacgcaacg ctgtggaggc ctcggcgaga
     5521 ccgcgccggt gcgtctcgat acagggaatg cacggcgttc gccgctcgac gaccggcggg
     5581 gtgatgcagg gcaaagaacg gtgcaggatc gccacctgcg cccgtttact gagccgtttc
     5641 ctgctcctcc cgtcccttcc ccagcccctt cacatcctcg atgacgaggt agagacaggg
     5701 cacgaggata agcgtgatcg cggtggcgaa gaggattccg tagccaaggg agattgccat
     5761 cggaatcatg aaacgcgcct ggcgcgaggt ttcgaaaatc atcggcacca gaccgcagaa
     5821 cgtggtaagc gaggaaagga tgatcgggcg gaagcgccgg attcccgcgc ggtggatcgc
     5881 gtcgtgcgcg ttgtcgccct ccaaccggca ccggttggcg taatcaatca tgacgagggc
     5941 attgttcacg acgacacccg cgagcgcaac catgcccatc atgctgatga ggtcaatcga
     6001 atagcccatt atcaggtggc caagcagtgc gccgacgatg ccgaacggga tcgccgtcat
     6061 gacgatgagc ggctggctgt agctccggaa cggaatggca aggagaacgt agatgacaag
     6121 aagcgccagc aggaatcccc ggtagaggct gtccatggac tccgtcatcg ttgcctgacg
     6181 cccctggaaa ctccaggaga gccctgggaa atcgcgcacc agttgcggca gaacttccat
     6241 ctggagcgat tccagcacgc gattggtttc cttctcggga atcacgcttg ctcgcaccgt
     6301 aacggtgcgc cgcccatcgc gccgcgcgat gctggtgaac gcccgccccc gctccacctc
     6361 ggcgacttca tagagcggga catcgcgacc ggcaggcgtt cgaatcataa gctgttccac
     6421 atcgtactcg ctgaggcgct ccggttccgg gagccggacg cggacggtga tctcgttgcg
     6481 cccccgctgt tgccgcaacg cttccgtccc gaggaacgaa ttgcgcacct gccgcgccac
     6541 gtccgtcggc gtcaacccga ggctcaaccc ttccggtctg acgcggaagc tgagctgttg
     6601 cttcccgggg ctgtacccgt cgtcaatgtc cctggtctgc gtgaaatggg caagtttccg
     6661 cgccaggtcg cggctggccg ccgccagaac atcgatgttg gagtgcgtca gttccacggt
     6721 taccgaggaa cctcctcccg ggccgcctcg gtccgactcg aatttcaatg actgaagacc
     6781 gggtatctgg ccggtctttt cccgccagat gtccgttgtc tcacccgtgg aaaggggacg
     6841 gatatcgggg tcggtcaggt agatggccac ttcgacgacg ttttcgttga tattcgcgta
     6901 aatcccctgc acgagcgcgt cgccgtattc cgcacgaatc ttctccgcgc tttccacgag
     6961 ccggtcgcgt acggcaaccg cgcgctggaa agggcttccg tagggaagcg ttgccgtgac
     7021 gtacgcgcgg tcggattcca ccttcgccat gaggacaatg cccatccgtc cactggcagc
     7081 gtatgcaacc actattgtca acacagccac gaatatggcg attgtcaggt atctccggcg
     7141 gagagccttt tcgacgaacg ggtcgtagac gttgttgatg aaccggtaca gcgcaccgct
     7201 gactttttgc tgaaacacag ccacgtggtg catggcgctt ccctcgggcg cggcgagaga
     7261 gcgcgcgagc tgggcgggaa ggatcagaat cggttgaatc cacgcgatca gcgttgtgaa
     7321 gatgaccacg aacggtacaa ctctccagat ttttccgatc cagcccggca tgaacaggag
     7381 gggaacgaat gacgcgatgt tcgtgagcat gctgaacgca atggccaggt tgacttctcg
     7441 cacgccctgc actgccgcat cgagtacctc cataccccgg cggcggtact cgtagatgtt
     7501 ttccccggca acgatcgcac tatccacgca caggccgagc gccatgataa aggcgaacat
     7561 gctcatcatg ttgatggaaa cgtccagcgc cggcatgaac aggatggttc caagaaacgc
     7621 ggtggggatg gccatcgtaa cccacagcgc gagcttcggt gcgaggaaaa ggccgagcac
     7681 cagcaacacg agaatgagac cggacgcggc atttcgaaga agcaggtcga ggcgctgacg
     7741 atacgtgaca ctctggtcca tgcgaaatgc atagtgcacg ccgggaggga aatccggcgc
     7801 aatttcctca agcgcttcat gcgccgcgtt ggacacgccg atgggggtct gatcgcccac
     7861 gcgatagacg tccacattga cagccagttg cccgtcgaat gtcgccaggc gattgctgtc
     7921 ctcgaaccct tcgcgcacct cggcgatatc gctcagatgc acgacgacgc cggagggtgt
     7981 cgtgatgatc gggatgtctc cgaattcgcg ggcccagtcg cgcctctcct tgacgcgaag
     8041 gaggatctct cccgcgtcgc tcttgatgct tcccccgggc aactcgaccg ctacctgccg
     8101 gatggtctgg gcgacctgcg ccagcgtgag gttgtaccgc cgaaggtttt cctgagaaat
     8161 atccacgtgg atttcgtaat cgcgcacccc gctgaggtca acctgggtaa tccccggttt
     8221 ctgcagcagc ttctcgcgca cgagctcggc aacatcacgc agaacccagt cgtcaacatc
     8281 tccgtagata agaatcgcca ggacgttccg ccgcgagatt gccagcgcta cctggggttt
     8341 ttctgcgtcc tggggaaacg tggatatccg ctgtattgcc tgctggatat cttggtacac
     8401 gcgctgggca tccgcgcctt cttcgatttc caccgacacc gagccggagc cttcggcagc
     8461 cgttgccgtg atctgtttta tcccgtctat cccgcggatt gcctcctcaa cgacgaggat
     8521 gatgccctgt tccacctcct ccgggctcgc accgggatag ggcacccgga tgtcaacccg
     8581 gtcgagttcg aactccgggt acacttcctg cttgatccgg tacgcagtga caagcccccc
     8641 gattatgaac acgagcataa gcagagttgc ggtgactcga ttgtgcacca tccacgcaag
     8701 gggtccggtg tgctgcaccg gcgggaacct gttctccggg ctgttcaccg gctcgtcccc
     8761 ctcgtggcgt tgcgcgctgc cggttcctcg cgtgtgcgga gcggctggcc ttccaccgcg
     8821 acgctgaggt cagtggttac gatgcgttcc ccttcccgca cgccgccggc tacatagaca
     8881 acgtccgggc ctttcagggc gatggttacc ggtcgaatct cgaggcgact gctgtcattc
     8941 atgatccaca cgcggtcgcc ttcgcgaagg aggtcccggc ggattttggc ggccgagggc
     9001 accggattgc cgcgtatggt aacgcgcaca taagcgccta ccagcaggga gggtttgccc
     9061 tcgttgttcc gcgtgagggc aagcggatcg ggcacggaga tgagcacgcg cgccatgcgc
     9121 ccctgttctt ccagatcgcc ggccacccgt gccacgtatc cttcccggac caccccgctg
     9181 ccccattcga catcgttgga gatgactgcc ggcgagccct tccctgtcgc ggattgcgga
     9241 acgttgatcc accgcagctg gttcaccggc acagacgccg tcacccagta ctcatccgtt
     9301 cccatgagcg cagccagtgg cgagttggcg gaaactcgcg ctccggcgtt gacatacgcc
     9361 accttgacga ttgcattgaa cggagcaaag acactggtgc ggcgtcgatt caaccgcgcc
     9421 tgcgccagtg ctgcttccgc tgacgcgagc gccgcccgcg ccgtttcaag ctgcggctga
     9481 cgaagcgcaa gagctctctc cgcctccccg ccctgctgcc ccagaagctc aaactcgcgc
     9541 cgcgcgaccg cctgtttccc catctcggtc gcaagttcac tgcgcgcgcg ggttacctcc
     9601 gccgcggcgc gttcaactgc aaaatcgtag tccgcggggt caatctggac gaggagttgc
     9661 cccttgcgga atcggccgcc cggaatgagt tcggggctga tactcagaac ctcgccggcc
     9721 accggtgccg caacctgtac ctcctgcgcg ggttgaaccg ttcccattgc ctcaatggtg
     9781 accacgtgtt ccgcgaaatg gagggtatct acctcgacga ggcgggcgtt aaccggccgc
     9841 tggcgccgtt ccgccctcgg ggctgttttc atgagataga acgcgacgag cgaccccgcg
     9901 atcacgatcg ccagtggaac cgccgcccgc agaatcctcc tgccaccttt ctgcgggatg
     9961 ggtgtgggtg tgctgctatc tgccattttg ttccgtttcc tgcgaaaagg ctttggtcac
    10021 cgcgagcctg tgagtggtcc ggggcgggtc agctcccaac cgccgctgag ggctctgtag
    10081 agggcgatgc ggttctcgat gagcgcaagc tgcgcgccca ggtaggagat ttcgagcgcc
    10141 tgatgtgtct gctgggcttc aagcacgcga gtgtattcag taacgccctg caggtaattg
    10201 tcccgggcac gattgatcac ctctgccgcg agggaaagct gctgtcggag gctggtcacg
    10261 tattcctgct gccggcgttc cgtggcgatg gcgttttcga cctcggcgag cgcatcgagc
    10321 acggtctgcc cgtacgcggt aaggcttgcc gatgttgcgg cccgcgttcg ctccacttcc
    10381 gctttgcgcc ttccaccgtc caccagaggc atggcgacat ttgccgcgag agaggcaagc
    10441 cagttcgaaa acaggtccgt gacggattcc gcagaggtgt tcgctccggc ggagagggta
    10501 atccggggaa aacggtcggc aatcgcggcg gcggctcggc ggtctgcagc catcacctca
    10561 aaatatgctt tgtggacgtc cggccgccgc cggatgaggt ccgccggcac acccgccgca
    10621 ggcatcggcg gaaggtctgg aagatcgggg ggtggacgga aggcactcgt gccagggttc
    10681 tggcccagca gaacggcaag gcggttgcaa aggacggccc tgtttgcctc ccctcgcgca
    10741 atgtcgccgc gacgcgcttc aacggcctgt cgctggcgta atacatcctc cgcttttgat
    10801 tgcccctgct ggaaccggag ggtgaccagc tcaagcattt cctcgttcga tcgaagctgg
    10861 cgacgcagga ctgcggtttc cgcctcctgt ccggagatcc ggaaccatgt ggtggctacc
    10921 tgagccgaaa gggtaacggc agccgtgcga acgtcttccg cggacgccct cgcatccaga
    10981 agcgaggcgt cggaagccgc acgcacgcgc ccccagagat ccacctcgta actcgcggcg
    11041 gcgccgagag agaattccgt gtccgtgccg ttcgtggcga cgttgccggt gtgctgtcgc
    11101 gtcagttcgg ccccggcgga aagagacatg ctcgggtagg cacccgcgcc ctcacgccgc
    11161 gccgcggctt ccgcctgcgc aagacgaaac cacgcgcccc gaagagaaag attgtctgcg
    11221 agggagcgct caacggcttc gttcagcgcc gcgtctccga aactctgcca ccaccgatcc
    11281 gccgcttcct gcgttcccgc ggcggaaaac tcagccgggg gagcgaccgg tggagcaatc
    11341 cccgcgggct ttggtccaca ccctgcaatc aacgcggctg gcaacacgag atacgcgatt
    11401 acgctgtttt ttgcgccaca accagtcact cgttaccttt ctgcgcattc acgacacatc
    11461 acagaactgt tttcgccagc aatgcgccga gatcgcgcag ttttgttcgt gccgcatcgt
    11521 ccggcttgcc gcgcacaagc agcgtctggc cgacctgttt gaacctgaac gagcggcaga
    11581 tcttttccac gcttgtggag gctgacccgc cgcccccgtg cgtgatgaag atggcacagg
    11641 gcttatcgtc cacctgcccc tgcgtcgggt agaacgtgcg gtcgaagaaa tccttgagca
    11701 tcccggccat gtagctgaaa tagtcgggtg agccgaagca gacaccgtcg caggccagca
    11761 gatcagatgg accggcttcc gcggcagttt ttactactgc gtctgcaccg gcaacggatc
    11821 ggatcccctc ggccacaatt tctgcggcag ccgccgtgtt gccgcccatt gagtggtaca
    11881 ccacgagcac ggttcttgcc atggaacacc tccctgtggt ggactgtgaa gagccagtgt
    11941 atgtgggaaa tttacacact gcggtgtatc ggggccaaaa ggcaagcacg actcacggct
    12001 caccatttcg cagcagtccg catggggcct ttcaatgggc tcagtatcca caacggcttt
    12061 ccggggccct gcttcagaag tccggcactg aaacccgcgt cttccacatc cgagcccggt
    12121 ggcgtagttt tgatgtggcg cttccctttt cgcagtcctc cctctgtagt ttggggcata
    12181 ccgatggaaa ctccgtttcg agtccttgaa atcggcacga cgccgcagtt gcttattgac
    12241 gactacgtgg tagaggacgt ctggatggtc cggcggtcgc ccgaggaacc ggtgaagtac
    12301 ctcggcaacc ccatttttcc cgcaggtgat acggttatct acgacgagga ggaaaagctt
    12361 ttcaagatgt ggtacggcat cggcatcccc ggaaccttcg aagcgaaggg ggcctacgct
    12421 gtttcgtccg acgggctgca ctgggagaaa ccggagctgg gactggttga ggccgacgga
    12481 tccacgcgga acaacctcat tgaaacaacg ggattcgggt cggtgttgaa ggacccgcat
    12541 gaccccgatc ctgcccgccg ttacaagatg atgacgaagc gcgcgggaac gcctgagtcg
    12601 gaaggaaggg cctttgcggc attctcgccc gacggaattc actggaaaga ccacccgggc
    12661 gagaaatcca ttctccgggc atccaatgac ggcaacggga tggtgcttcg ggatgactct
    12721 accggcaagt acatcaactt tcgccgcacg acggttctgg cgggtggaag gggagtactg
    12781 ccggaggata tcgggttccc tgatccccgg gtaatgaagg gcgcgggcgg ggacaagctc
    12841 ggggttcccg agggcatcgg atttccttcg gaggatgact tcgaggcgta tgaggaggcg
    12901 gaggagtata tccaccgcta cctgcggcct gcaccctacg tgcatacgcg gacgctgcgc
    12961 atctttcggg acactcatga agggcttggg tgcaaccgac ggatcgcgcg gtcggaaagc
    13021 gacgacttcc tccactggac cgaacctgaa gtcatcatac gtcctgacga gcttgacccg
    13081 ccacggctct actcgataag cgtcgccagg tatctcggga tgtacatcgg gcttctggag
    13141 ctgtacgatt cgtgcgggaa ccggcgcgcg ccgggcactc ctcaggaatc cgacaccatc
    13201 gatacccagc tcgtattcag ccgcgacggg tggaaatggg aacgccttgc gaaccggccc
    13261 gtgttcctcc gccggggcat tattggcgcg ttcgactgcg ggatgctacg tccggtcaac
    13321 cccccgttcg tggtgctggg cgacgagatt cggatctact attacggcca cgggagcagc
    13381 cacaatattc cgtcgccaaa tcaaggcctg ggcgttgcgc gactgccaaa ggagcgattc
    13441 gtcgcgcgcg tggcgggaga tgaggtcggt gcgctcatca cgaaaccgtt ccggctggag
    13501 ggcggagaac tgcagatcaa cgcgaacgcc gcgaaagggc tgatcaaggt cgaggtgacg
    13561 gatcccttcg ggaatccgct ggaaggattc agcgcaaagg aggccgtgga aatacgggaa
    13621 aacgggttcc gcgtgcccgt gcaatggacg tgcgggaaac ggctttcaga aatcgccggg
    13681 cgagtcgttc ggttgcggtt ctatctctac cgatcgagat tgtactcgtt cgtggtggac
    13741 gaagggaggt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcccgccc
    13861 tcggcagcgt gaagccgccg tccactttga gcacggaacc ggtaatgtat tccgctttct
    13921 ccgaggcaag aaacgccacc gcctgcccga tttcctcaat ggtggcaagg cggccccagg
    13981 gcagcttcgc gccttcgcgg gcgatatcct cttccgaact ccacttccgc tcgcccggcg
    14041 tatctaccca gcccggctcg acgatattga cccggatgcg atgcttcgcg agttcagcgg
    14101 cgagagtcat cgccagagcg tgtccgcctg ccttggcgat gttgtaatcc gcacagttgg
    14161 cgtacgggaa cggcacatga acggagctta cgaacaccat cgcgccgccg gtgttctgcc
    14221 ggaccaagag ccttgccgca gcctgcgccg tgtgaatgac gccccacagg ctcacgtcga
    14281 gcgtgtgagc gatttcctcg acgctcagat caacaaacgg tttgcacact gtccgccgcg
    14341 ttggaaacga agaggtggag gccgccgaag tgctccaccg tgcgggcgaa catggcatcc
    14401 accgcctccc ggtccctgac atccgccatt gcggtgactg cccgaacgcc gaaaccgcgg
    14461 caccgctccg ccactgctct ggcctcgtcc tcgtgggaac ggtaattgac gacgacactc
    14521 cacccgtccg cggcgagcgc ttcggcacac ccgcgtccga tgccgcgtga agcgcccgtc
    14581 accagcgcga ccttttcgcc tttttccatc gttttccctc ccgaagggga acagaagcca
    14641 caggaatgaa acaagccccg catccttcgg acgcggggct ttgaaacggt ctgggcgggg
    14701 tatcaggcgg cggttttctt ttccacgcgc ccgcttttga tgcattgcgt gcacacacgc
    14761 acgtgcttgg aagtgctgcc gaccatcaca tggatgcgct ggaggttcgc cttgaagagc
    14821 cgtttgcgcc ggttgttggc gtggctgacc gtattaccgc gttggaagct tttgccgcaa
    14881 agttcgcatt gataagccat gagtgaaatc ctcggttcgg cacggagatc gggttttcag
    14941 aaagatttta atgatagaac aggaaacgcc ttttgtcaag tcccgggcga gccgggtcgc
    15001 gcggtcggca cgcacgaagg ttgaccttgc gcgggcctgg cgctaccttt ttcttgcgtt
    15061 tttgtcggtc cgcctcctcg cggggagaga ttcatgcata cctggggtgt tgccgttcta
    15121 cttatggtgg tgacgtgggt ggtccttgcc gggctgcctg gcgcgccggt tcaaagcctc
    15181 gcggggaaac tgatccttgc catcggtttt ttcgtgatcg gcgtcggttt ctacctcctc
    15241 agtatcgggc cggttgacag cgtccaatcc atgacgtggg cgccccttgc cctcgtagcc
    15301 ggcttctgtg tgatcgttcc gctggggctc ctctatcacc gcagggcgcc aggttcagaa
    15361 gaagacgcag caacgaaaac ccgcctgtaa atccggggcg cttagctcag ttggttagag
    15421 cgtctgcctt acaagcagaa ggtcgctggt tcgaatccag cagtgcccat tacttttgcc
    15481 agattcgccc gcgcggtcgc gccttaccag catgagtcgc gttattcagg ttgtcgggct
    15541 ccggcgcctg aatccgaccg ggaagtgcaa cgtcgtgaga attccagcac tcgttcgatg
    15601 atctggacgg catcttcgac gatcggttca atttccctgg ctgcatcagc cggaacctct
    15661 ccgtaggctt tcagctttcg gggccggaga tcttccagcg tttcccagac gctggacatt
    15721 gttcccgaga catggcgact gaccgaacgc tctgcgggag acactcccgc gcggtcggca
    15781 aaactgacaa gtttttcgtg cagttcgtcg cacatctcca ccagcctgcg aatctggtca
    15841 tccgagtact catcggcgaa acgtgtcatc cggttttcgg aggtctcagt cagcagcctc
    15901 ctgcagaaga gaatctggtc ttctgcatac cggagtgagg aacccactat tctccggtga
    15961 ttttcggaga gaggaatctg cggttttttc atggtgcggc tcccgtgggg ggcggacgca
    16021 acgagtgttc cacattttca agctgccgga tcgtttcctg aattccgtga atgagagatg
    16081 cggtttgttc ttcgtgcagc ccgcgttttt cggtggctga gttcagtgcg ccgttcgtcg
    16141 ccgtaatcgt ttctcccaat cgcgtcacga gtgtgcttcg gaaatgccgg aaggtttcgt
    16201 ccaccgtccg gagcatggcc caccggaggt tctcggcgtt cgaaatcacg agatcttcaa
    16261 gctgattgtt gagacgtgtc tggaggcgat gcctgcgcac ccgttcaggg agaaacctgt
    16321 cccagaaccc ctccgggatg gggctcagcg acgtagacca ggtccgcgcg atccagtagg
    16381 gcttctgcac gatatcgaag gtgatcactt tccctgccgg ttggtacggc acctcgaaaa
    16441 tgctggccgc tgctttcctc acggattcga ccagctcttc cgccctgctc tggtgcggcg
    16501 tcagcacggc gtcgatctct tcacgcagga aggcactggt ctggcccacc ttctgttcga
    16561 aaaagacggg aatcgcttct gcgagggcat ccctcgcatc gctttcggcc tgttgcgtat
    16621 ccgcgttgcg agccagagcg cctgcaacaa tcccccggaa gcctgttcgc gcctgcgccc
    16681 gcaccgcgtc aatgtactcc tccagccttg ccctggtgcg tctgtgatcg cctttgagca
    16741 ggtcgtccgc gacaaggcga tttcgttcga tttcgtcgag cttctggcgg aagaatgcgc
    16801 gctttttgtc gagttcttcc gcggggagct gcagacttcg cagtataagt cgaaactggg
    16861 taagtgcttc ctcgacagtg tcggccgctt tgtgccggac cgcctctgaa agagctgctt
    16921 tcttttccga cgcgaggaac cgggaaagat gccgctcgac ccggtgcatg ccgcttctct
    16981 cccagaggtc ggcgtcggca ttctggcggg cgttcaatcc cagctgcgca gaaaccggaa
    17041 atatcgggtg gtcatcagaa aacccgagtt tgtctgcaag caccgatttc agaaagcccg
    17101 tggcttcgcg cacatcggct tccgtatggt agtcgatctt gttcaacact acgaaaagcc
    17161 tgttgatgcg gcgcttcacc tgccgcacaa actcaacctc cacttcggtg atgggcgggt
    17221 ctgccgacac aacgatcatt gcggcatcgc actgcgggag gaagttgagc gttgcttcgg
    17281 tgttgtgccg gtgcgtggat ccgatgccgg gagtatctat cagaacgacg ccctgtctga
    17341 ggatcggcgc gctgctgaag acgtccacct gagttactcc tcgctcattt ttcggattgc
    17401 cgctttccgt gacaaactgg gcgatgaatt cccgaattgc gcccgcatca tcagcttcga
    17461 acgcgcgggg cggcacgccg ttgtcgaaat acacgagcgc acgtcttgtt tctcctgcgc
    17521 gaacgaaggt aggaattgcc gtgagcggaa ccactgagga ggggagaatc gcttcgccga
    17581 gcagggcgtt caggagtgtg ctcttgccac gtttgaactg gccgagtact gcgaggtgaa
    17641 atcgctcctc ggcaaggcgg gttttcagtt ccgcaattgc gtcggaaaag gtcttgaatt
    17701 ctggtcccgc agtgccaagg atcgacagtg cctcatcaaa gagctcgccg aggccatttg
    17761 cgttcgtgat atcgccgttc tccccgccgg cggtctgcgg ggcccggaca tccgtttcag
    17821 ggatacacac agttgctcct cccgtcgttg tggtgtcggc aggagtcatc agcccggcaa
    17881 gcccgggcgg cgcggggact ccatcccctc aaatggccgg agagcctgcg ctcgttttcg
    17941 gagcgcaacg ctcgatttct tccgtcaggg acgtgacgga atccgtcagg cagttgccgt
    18001 ggaaagaacc ggcatgtcgc gtccacgcca gagaaaccag agcgttcccg ccagaccgaa
    18061 cgcggaggcg ctgatgaggt tcaccgcagg tgatatcatc cagaggaacg ccccgccgaa
    18121 agccgcgagc gacacgacgg aatctctcag caggtaatac aggccgaaca tccctgcctt
    18181 ggcaccttcc ggcgcgagat ccatgatcag tgcttttctg gtcggttcgc cgaattcctt
    18241 caggccgcgc aggatgaatg caccgacgag cgggagcagc gaatggctga acagaagcgc
    18301 cacaggaaaa agggtgaagt tcacgaaggt gattacgacg aagggctttt tcccgctttt
    18361 gtcggcgaag tatgcgacgg gaatgtaaca cagcactgca gtcgccattt cgatcgtggt
    18421 gagaacgccg aattcgatcg cgctgacggg attggaaatg accttcatgc accagataac
    18481 cacgaatgcg taaggaatct gctcgcagaa ccgcaccagg atatcagata cgaggaggtt
    18541 cctgaggtcc gcgttcatca gaccgatgag gcggaacggg ttgccggaga ggggagctga
    18601 agcctcttct cttttgtcct caatcagcaa ttgctgcata accatcgcca ctgcggcaaa
    18661 aatgaacgcg gcgatgaatg ctgctctcac accctcgcgt tcgccccaga tcgagatgaa
    18721 tgcgccgcct accagcgggc ccagcgccat gggaatccgg cgcacgaggg agtgcatcga
    18781 aacacccatc gtccttttgt ttttcggcag gacaaccgcg acgaggccca tcgtggccgg
    18841 gagggagatc gcggtccatg agaggaagaa gaatgcgccg atgatcactg cttgccagac
    18901 cgggatgaag atcaccacgc agtatccggt catcgccatc aggttgaaca cgatcagggc
    18961 gcgcttcgtg ccgatccgat cggcgaggta tccgcccggg aatgagtaaa gggccgaaag
    19021 gaggttatcc agcccgttca ggaggccgac ggaaagaact cctccgccaa gagcgaacag
    19081 atagatcggc aggaatcgtt cggccatgcg ctcgcccatg ccgaccagca ccaccatgct
    19141 cagcacgccg atgatacttc gcttcaggcc aagaaaatcg attgcgttcc tgatcgccgc
    19201 catttcaggc gcctttcaaa ttgagaagcg agcggtccct ccgcagggcg atgtgcccga
    19261 ttgaggcggg agagcccttt cacttcggaa tatgcctccc gcgccaacgc gcccccaaca
    19321 cctgttctgc ctcggtgcgg tgaaccggcc gcgtgatggt tactcttctc caacgtcctc
    19381 ttcttctttc gggggaagcg ccccgcggcc ggcaggttcc acggaaaccg ttgccgtctc
    19441 cggcaggaga tgccgcggca tgaaggccgc attcgcgata agcgtcggaa tgacagcgct
    19501 ggcgatgact gtcgccacaa ggaacgagta ctgcgattgc gtcacgatgc catgactgaa
    19561 tccgtagagc gcggaaattg tgccgaacgt caggcccgtg gacatcatga gtgtgtagta
    19621 ccagcgttcg ttcttctccg ttctgaatgc cgagacaacc ggcagaagcc cgaaaatctt
    19681 cgagacggtt ttcgccgcca ggaacacgat gaacactgcc ggcgcggcga tgagcgcggg
    19741 aagcgcgacc agagaaccgg cgcgcaggaa gtaaaacggt gtcagaaacc cgaccgtgag
    19801 ggtacggagc cgccgcatga aatgggcatc gtcggaggtg aactcggcaa ggaccatgcc
    19861 ggcgatatac gccggaagca ccgcttcact gccggaccac agggcgaggg caccgagggc
    19921 gaagagaacg aacacaatcc atttggtgcg gattgccgcc gttctgtggc cgtagtgccg
    19981 cgtcagaaag gacgttgcgt acggcaggat gaaaaaaacc acaaggcacc cgccgatgaa
    20041 cacaaacgtt ctgtaggtga aaggggcgaa gatcaggcca agggcaagca ccgttccgag
    20101 atcgttgacg aagcaggcgc ccagaattcc cttcccgaac tccgtcctgt tgaatccggt
    20161 ttccagcatg actgcataga ctaccgccat cgaggtagtg gagagcgcga ttcctgccag
    20221 caggctggcc cgcagatccc acccgagtcc gtaatgggcg accagcgcgc aaccgacgaa
    20281 gggcgccaga aaaccgacaa atccgattat agatacttcc ttgagcttgg tcttcatcac
    20341 ctccggttcg agttctgcgc ccgccagaaa cgtgagaagc accgcgccgg aggacgcgag
    20401 gaacttgagc cattcttcgt tgttcggaaa cgagttggcg ccccagaagt gcgtgattat
    20461 ggccgcggca gccactccca tacagatttc catcagcgcg atggatactt tcaggtggtt
    20521 tgccaacacg gcagagatca ccgccaggaa aagccagacg gcggcgtggg tgaacagggt
    20581 ttccatgagt cgacctctca gaggatggcg atggacacaa aaaccgccag aacgcgggaa
    20641 acaaaaaact cccgccggcc agtactcggc tgcaggagtc atcagtccac cgttcgcgga
    20701 cggttcaatc ggggaactcc atccccgtag taaatgtacg caggccggga gccgcgggca
    20761 atgatttctc acaattggcg cgcgtcgtgc agaccttgac agtttcgccg gcacgcgata
    20821 cactttaatt gcacgcaggc gcgaatcagt gcattcaaga tgccacacag aagcgacggg
    20881 agaatccgta tcgcacgatt gtgatgagag ttacgacgag tggttccgat gaaagccccg
    20941 tccgggcaca agcggaaacg cattgaaaaa gctgttcgtc gcgtggtttc acaatcgtcg
    21001 ggttcctccg ccttgcaggt actatgagcc gagctggttc agggcggaac ctctgaacgt
    21061 ctcggctctc ggtgttttat gggtcttctc cgcggtttcc tgatgccttc cccgggacgg
    21121 gcaatgctgg ttggagcagc gttgtcggtc tgcgcggccg ggtcggcccc ggcgcgctgg
    21181 gagttgattc aggcggatgc gtcaggcgtg gttgcggagt ggtcgtcgcc cgtggtgcgg
    21241 atggacacag tggttgtggc ggggcagcgt acgctccagc cggtgcttga ggattgctcg
    21301 gcggacggcg aacccggctc gccagccatt ccgaaggcag gtacgctgat cgcggttccc
    21361 gatggggcac aggcgtcggc ctctgcagtg gcgggcgaat gggaaacgcg gcccgggctg
    21421 ctcagtcccg tgccgtcaca cgagctccgc gaggatgggc tggggtaccg ccgggtgttc
    21481 gagatccgcc cggaggcgta ttccgcgcag tatacgggga cagacctcgt atcgcttgaa
    21541 cctgccggcg aagcctacgg cgcgcatctg gtgcgtatcg ttgtgcatcc ggtccgggcg
    21601 aaccttgccg acaaaagcct tgcaatcacc cgttcggtgc gcgttcgggt gcagttcaca
    21661 tcgcccgaaa ccgggagcct gcgggatatc actccgttgc cttccgtctg ggaaggagcc
    21721 gtactgaatg ccgcagcgta caatgcgctc caacggacaa gccgcagcgc ggccgaccgt
    21781 gtcgcgaggg ttgcggaaac cagcccgttt gcctccgggg tgtggttgcg ggttgtcgtt
    21841 gatgcggacg cgatgtacaa gatcaccgcg gcggcacttg ctgaagcgga cagccgcttc
    21901 cggagtgcgc ccgcggaaag gctggcactg tatgccggga gcgggcggga actccccttg
    21961 gacccgcaga aacctcgtca gaccgccctc cgaccggttc gcccgatcgt ggtggatgcg
    22021 aatggcgacg gggtgttcaa cggaaccgat tatctgctgt tctacggccg cagcccgtca
    22081 ggatgggatc tcgattacca gacactggat ccggtgtatg cgctcaacca ctacacgtac
    22141 gaaaacgtct actggctcac catatctccg gcaaccgcga tacgtgcgga ggttcgcaac
    22201 ggagcagtga gcgacccgtc gcttccggta attgaacgtt tcccgtttcg atttcacgaa
    22261 gagcctgagg taacgaatct caacgaggaa ggtgacactg acgggccgta ttccggtgtt
    22321 gactggcaat gggatcagct tgcgccgcag gcttcgcgcg tcctgcaggt ggcgttactg
    22381 gacgttgccg gtgacacggt cggggtccgc gtggggcagc tacggcagtt ttcggcgtac
    22441 gggtcgttgc aggtgaaggt caacggcgtc gtcgagcccc ttgttgagca acgacagact
    22501 tccacgtatg cgtggtgcac cgggtatgcg ccagtgatca ccccggccgc gcaggtcagc
    22561 cttcctgtgg aaatccgtaa tgcgagcacc gggtatccga tcattctgga ttactacgaa
    22621 atcgcctact ggcgcaggtt cgtggtgcgg gggagcagtt tcgccttcgt gttgccgccg
    22681 ctgcgcaaca caatcccatc aacgcgagtc gtggtgcctc tttcaggagt cgaccggaca
    22741 ctgcatcgtc tttttgaagt gagtgacgac agcggccttg tggagtacgc gcttccgcca
    22801 ccggacgatc aggggattac gcgggtggaa ctcaggcaat cagggctgat cgagcgcaaa
    22861 taccttctgg ctgccgaatc ggactggaga actccgctcc ggatggaacc gaaaaccggc
    22921 gcgaccaatc tgcggggtcg taccgccggg gtggactacg tcgtgataac ccaccccgac
    22981 ttccaggcgg aggcatcccg gctggccgat ctgcgggcgc agaggaatgg attccgcacg
    23041 gcggtcgtga cgacgcggga catttgcgat gagttctcgt tcggaatgtt tgatcctgca
    23101 gcgctgcgtg atttcctgcg ccatgcgttc atgaactggc gagatggcgc cgcagaccag
    23161 gggttgcggt acacagtgct gcttggtgac gggcagtttg actaccgcaa tctgacgcgg
    23221 ttcaccacct ccacggcgaa cccgcagcca ggaaactggg tcccgcccta ccaggagggg
    23281 ccggtcacaa cggacgattg gttcgtggtg tttgacaaca cggggattgc atccgtcaag
    23341 atagggaggc tctgcgttca gacgaaggag gaggcgcggg ccgttgtcgg gaagatcatc
    23401 gcgtacgagc gtgacccgga gcgcggtgcg tggcagaatc gcgccatcat gattgccgat
    23461 gatgagtacg atctcgaccg gggaatcaac tccgaagact acgtggacga tagcgaacga
    23521 atggccgggt gtctccgtgc agaaaccgtg acggagaaga tctactcgca cgaacggcaa
    23581 ctgaaccagc agatgcagaa gccatccgtc aacaggatga ttaccgacgc gtggagcagg
    23641 ggtgccgcgt tgatcaacta cgtcggccac gggaacccgg tcctttgggc gcacgagcgc
    23701 attttcacgc tcgacgggga ccttccgaac attgccaacg ggaagcgtct gccggttctc
    23761 acggcgctga cgtgcagcgc ggcgcatttc gatgcgccct cgacgcagag catggcggaa
    23821 gtgctggtaa acatggcgaa tggcggtgca gtagccacgg tggcggccac gcgtctttcc
    23881 tacaacaccg aaaacgttgg gttcaacgag atcttcctct cgcggctgta cggtggcgcg
    23941 gaaggcagga cgcctcgaat cggggatgca ttctggtacg ccaaggcgct ggccgcgcag
    24001 aacccgagct ggctttaccg ccagaacgcc cgcaaatacg tactgatcgg tgatcctgcg
    24061 cttacggtcg cacttccgga gctggtggta caggtgcaga tgatgggcga ttcgcttcgc
    24121 gcgcttgcgg cctccacgct tcgcgggtcg gtgctggtgc cgggagcgac ggacacactg
    24181 cgaagtttct ctggatcggc gctggtgaat gtgttcgaca gcgcaacccc tatgagctac
    24241 caggccgccg gtggaaacac gatcctctac acaaaaccgg gaacgtcgat cttccgtgga
    24301 gtggtacctg tgcagaacgg tgtatttgcc accgaactca ttctgccgat cgaagtgagt
    24361 tacggcggca cacaggcaaa agctgtggcg caggtatgga atgaccagat tgacggtgcg
    24421 ggaagcattg gaggactggg aatttgcagt cgcccggccg catcggtgcg tgatacaacg
    24481 ggaccgagca tcgtgttcac gcgtgcagac gatctggacg cggcgattcc tctcagggac
    24541 ggtgccgaga ttccgagggg agagccggtc cgcgtatggc tttcagatcc gctcggggtc
    24601 aacgtgggcg gaggtatcgg ccatcgtctt gtggcgtatc tcaacggcaa ccgcagcgct
    24661 ccggtggatc tgacggaaga cttcgtgcac tggggcagcg cgaccaccgg ctgggccgac
    24721 attccgcttt ccggcgatct caccgcccag caggttgtgg tggaggcatg ggacaacggg
    24781 aacaattttg ccgctgactc cgtattcctt cgggtgggtc gaggcaagga tattgctgtg
    24841 agtaatgtta tcgcgtaccc caacccgatg gcgcgggacg gggcgttcac ttttgtgctg
    24901 gacggcgtgg gcgcgtctac cgacgctgac gcgacggtgc gcgtgttcac ggttgccggg
    24961 cggctggtgg atacggtgac gaagcttggg gttacggacg gacctgtggt gataccgtgg
    25021 accccggcgt atcagttggc gaacggagtg tacctctatc agatttcaat acgacgtcgt
    25081 agtgacgggc ggaccgccaa ggctttggag agactggccg ttgtgggtcc gtaaggtttc
    25141 aggaggaagc gcacgatgaa ggttgcacga ttctcacatt tcacggctct ggttatcgca
    25201 ggtgcctctg ccgccgtcgc gtttttgccg tcggtttcga gggcgaatga aagccaggcc
    25261 gcggtgctgg cgcttctctt ccggccgggg gcacgcgaac tcgcgctggg cggcgccgga
    25321 gttgccgggg cgcgcgggtc ggcggccagc tattacaacc cggcgctgct ttcgtggcag
    25381 gcaatcgccg aagggcagcg atatccgcgc gccgtcggca cgacgtatta caagattctc
    25441 cagaacttcg gtctgaacga catgtattac atgtatttcc cgacgatgtt cagtgtgaag
    25501 gactggggac agttcagtgt gaacgtcact tacctcaacc tgggtgaaca gcagcgcaca
    25561 agcgaaggcg gggaaaacct cggcaccttc aacacctaca cactggccgt cggcctgagc
    25621 tatgcgtcga agattggcgt gcacacctcg gccggaatta ccgcaaagtg gttctacgac
    25681 catcttgccg atgccggaac ggggtttgaa aaaggcgacc ccaccggaac cggtttcgct
    25741 gttgacgcag gcatcgcgta tcggccatcg gaccgtctgt actttggcgc agccctcagg
    25801 aactacggcc cgaatgtgca gtacatcgac gcgaaacagg caagcccgac accggtgaat
    25861 ttcaccgtcg gcacctcatg gaaagccttc gacacgcaat acaacgatgt cactatcatg
    25921 gccgacctgt acaagccgct ggtgcaggac taccgcaaga gctggtatct gtccatcctg
    25981 cgttcatggg ccgacgaatc ggtgtacaaa gtcgaggaaa taccggacgg acaggggggt
    26041 gtgacacgcg ttgagcacag gaacaccctg cgtgaagaga cccgtcaggt ggatgtgctg
    26101 accggtgtgg agtacacgta cgcggattac gtggcgatcc gaggcggtta tttccgcgat
    26161 tgggatggtc agcgcaactg gatgacattc ggcggaggcc tgcggctgcc tatcgcttcc
    26221 acggctcttc tcgtggattt cgcatacgtg cactcgctga acggcaaggg ctcagatccg
    26281 aacgacggcc agcaggtata ctcaatcggg ttcacgttct gaaaactctg acggaacacg
    26341 acatttcccg taccctgccc ggcctgtgct gctgacctga taccgtcgcg ggttccggga
    26401 gcacatgccc ggcagaaccg acttcacgag tgtccttcag cataaggtcc atcatgcacc
    26461 accgggggct cctgttcgcg tttttcgccc tttcatcgtt tcttgcggca gcgtcggccc
    26521 gagccgttga aacgattcac ctgcttgccc tgcgggtgga gtttccgaaa gaggacccgg
    26581 acgatttcac caccggcgga aatggtcttt tcgacatgcg ttccggcgcg gaggcgctgg
    26641 ccaccgcagg agaagcaggg ttccggtacc cgttcgatct tccgccgcat gacgggcgct
    26701 ttctggaaaa ccacctgacc gccctccgga actacctcga aactgcctcc cagggacgga
    26761 cgaccatcac gtgggaggta ttcccaccgc accctttgcc cgcgtacaag gctcctcaga
    26821 cgctggcgag ttacggctct ggcggctcgg tggcggagca gacgcggaat tgggtacggt
    26881 ttctcaagga cgcactggcg gtctcacaga atgatgtggg ctcgctgggg cggttccaga
    26941 gcttcctcgt gttccatgca agcgtatcgt tgcaggggac tctttccgcc gagcttcccg
    27001 cgctcgtatt gacgtctcag gaaatcgcct cgtcgggtat cccgattcca ccagaggtgc
    27061 agacggcatg gttcattccc cagcaaatcc agcaacccgg cggcgtaatc ggtctgaatg
    27121 gcgccttcgc gaaaacgttt ctcgcctccc gcggccttcc tgtgctctca aacacacgga
    27181 ccggtggcgc ggcggtgggt gcgtggacgc tgatggatgt aggatccgac aacttcatcc
    27241 aacgcccgag aaccggttct tcctctgata cgatccgcgt gctggggttc gtgccgtgtc
    27301 tgccttctgc gtgggagcag atgcgcctcg gctggcttgc gccggaaaca gttcgctcgg
    27361 acaccaccct cctgcttgcg ggccttgccg cagagacatc cctgccacgc gcattaaagg
    27421 tgcccatcac cgaagacgaa tacctccttc tggaactccg ccattccgcg gttgaaacag
    27481 accggtacca ccccgcaatc gagtacagcg acggcgatac cagcggcgtc tggctgaagc
    27541 cagaaaacgg gctgtatgat gcgtatgtgc cgggaagcgg ggtgatcgta taccatgtca
    27601 acgaagcccg catccgccag tgggagccga ccaattcgat caatgcccag cccgaccggc
    27661 cgggaatcat ggtagtggaa gcggacgggt atcgcgatat cggggtgacg aatcttctgg
    27721 gacacccgcg cgcgaatgag ggtatcggga gtcgaaacga cccgtttccc gttaccgggg
    27781 aacggatcct gtaccctgat ggcgagcccg cacccggcca tccggcggtg atcacggcaa
    27841 acggtagccc cggtggggtc gcaatttcgt tttcgccggc aggtacggcc cgggataccg
    27901 ttgcagtgac agtgcgctgg cgaaccgcgg acactcccgc gcgaaaaggt cgcttcatcg
    27961 gaagcgctgt tgtcggcgga gttgccgtac gcagggttcc gctggacggt gtggaacgga
    28021 acctgatcgc agtggcggga agcgattgga acctgtgggc gttcaccgat tccctcgaac
    28081 ccgtgggcaa cgccaacggg ttgatcggcc gtttctgggg cgatgtcacc aggaaaccgg
    28141 tcatcgaagc ggacggatcc gttcttgtag tcggcctcac gacgaacacg cgttaccgat
    28201 atgcgaacgg ggcgtgggat ggcggtacac ctgtggtgca aacaccaccg gtgccgtcca
    28261 caatgacctg tcaccttctg cgcgacgtgc gcaaatcgga tatcacagta acgggttccg
    28321 ggactatcac tgctgatagc tatgctcgct tcggatacct cccggaagac ccgcctcccc
    28381 cacgggtagt ctggtcggcc accacggatt cgttgattgc gccgctgagc ctcggcgacg
    28441 tagaccggga cgggttcccc gacatccttg ccgtgagccg ccggagcgtg gatgtgttct
    28501 cccgcagtgg aactcgaacg ataacctata cactcgggcg ggcggattcg accgaggagt
    28561 ttgccggatc ggcgctctcc acgggagcgg gcaacaccat tgtggccggg aaaaaggggc
    28621 tcagcgtgtt ttcggggctg atgcgtcggc atcctgcggc tacaattccg cttcccgccg
    28681 ccgccacggg ctcccccgcg ttgtacgcgg ttcgcgatga aggcatccac gcggtcgtgg
    28741 gaaccgcgga cggctggttg ctcgatattc ctgtctcgca gggcgatagc gtcatctggg
    28801 gccagatgtg gggagatggg gcgtcgtcgt tcgcgctgga cgaaacgggg cttgtaatgc
    28861 cggcagctcc ggctgaggat ttgatgcccg gagatcgcgt cttcttttac cccagccccg
    28921 tcggaaaaga agaggggatg cttcgattct tcctttcggc acaggcagat gtctccgtgc
    28981 ggatattcac gagcatgggc gagcaggtct gggagacacg cgttcccgcc ggtgagacgg
    29041 ttgcaaatgc ggagaacgaa atccgctggc cgggaggcac acgtttcgcc accggtctct
    29101 acgttgcccg gatcaccgct cggtcggcag acggcagaca gggtggcgcc accattcccg
    29161 tcggcatagt gaggtgattg caacggtgcg taagggggtt ttgttctccg cttcgaagcg
    29221 gcacgtgtgc ggcatgtgcc ttttcgtgct gctatttctc gcggctccct cgatttgtgc
    29281 gacgccgatt gcggaatttg acccgcccgt gtctgctctc tcctggaaac gattcgaaac
    29341 cgagcacttt cgcatcacat atacttccgg tcttgagcgg gtagcgtctg cggtggcgat
    29401 ggcggccgag caagcgtatg aaccggtaac gcgcctctac gggtatcggc cttcgggcaa
    29461 aacggaaatc atcgtgcggg atcacggtga ttttgccaac ggatttgccg cctattacga
    29521 caaccgcatg gaaatctggg catctgctct cgactatgag ttccgcggca ctcacaactg
    29581 gatatgtgac gttactaccc acgagttcac gcaccttgtc caactcggtg cgtcgctcaa
    29641 gacctcgcct gcaatcccac aggtatattt ccagtggttc cgcctggaac ccgaagcgcg
    29701 aaccgacgtc gcagagggac ttcccaacac gctcgtgtcg tatgcggtgc ccacggtcag
    29761 catccccatg tggtttgccg agggcgtcgc tcagttccag gcgcaggggg cccggcatga
    29821 tcgctgggac gcacaccgcg acatgatagt ccgcgtcgcc gtgcttgccg acagccagct
    29881 ctcctaccgg cagatggaag gattctacga cccggacggg cgtgaagccg aaatggtgta
    29941 caatcacggc tattcgttcg tcaggtggtt ggccgcgaca tacggtgatt cctccctccg
    30001 cgcgttgagc cgcgacatgc gtgcgtggca ccggtgggac ttcggctccg cgctgaaaca
    30061 gcttacagga aaaccgggcc cgcgggtgta cgaagagtgg ctgactcagc tccgtgcggg
    30121 ctacgaggcg ttcgttgcag agcgaaaacc gcagcccgcg ggaacactgc tggagcggcc
    30181 tgcgtcacgc cccggcgcgg aatccggtga ccgcccgccc gtaatcaccc cgggaggggt
    30241 acccgccgcg tacgcgccgc caccggcgca cagcgcgcgg gcaagttatt accatgcagc
    30301 acccgtatgg tcgccagatg gcgcgcttct cgcatatgtc tcaaccgacg ggcaggatta
    30361 ccgcctcgga tcggtctggg tccgtaaacc cggcacaggc gaactgactg tcgcgccggg
    30421 atcgttccgt gcatcctcga ccgtaagctg gtttcccaac ggtcgcacgg tcgtgttcag
    30481 ccgcgctcaa cgcgacgacc gcaatgagtg gcgctacaac gacctcgtaa aggccgacct
    30541 tcagaccggc accgttacaa agctcaccga tcagtggcgc gcctcgtacc ccgccgtctc
    30601 cccggacggc gccaccgtcg cattcgtccg aaacgctcgc ggttccacca acctcatgct
    30661 cgcgaatgcg gacggatcca acgtacgtcc ccttacccgt tgggatgacg gcacgcaggt
    30721 tttttcgccg aagtggtcac ctgacggaac gcgtctcgca gtaagccttg cccgcgcggg
    30781 aagccgccac atcgtcgtct accgtgttgt ccgcgcctcc gggggagagg tcctcgaaac
    30841 cgaacgcgta attgcttcgt cggacgatga ccgggacccc gccttctcgc gagatggcac
    30901 gcgagttgtg ttttccagcg ccaccgacgg tgtgttcaac ctctatgagc ttgatctcga
    30961 acgcggctca tgtgcacgat tgacaaatgt actgggcggg gcgtttcacc cggacgtggc
    31021 tcctgacgga cgtattgcgt actcctcgta tgaatcggca ggctacgtga tccggatcat
    31081 accggcggat gcgccacgat atcccgtatc tgcggatata tttgcgaatc gtattccaga
    31141 acaggggact ttccctgaca gtatgcccga aggttcctct gtgtcggaca ttgacaggcg
    31201 cattccccat ctgcagagac cttcaatatt gccgcgtttc gggcgctacg gcaaccagtg
    31261 gcgtgtgggt gcgtacgcgt tttcatcgga tatctgggac gatgtccttt tgatgggcgg
    31321 agtctgggcc gcgccggaaa accgcgacta cgatgcattc gtcaccgcgg aagtagacca
    31381 catgaccccc gttccggtgc tgtttgatgt tgtcaggacg gtacgccaca cgaaggagga
    31441 tacgaccttt gcgggtcgtt tgcggctgga tgggatcacg tacggtctga acgcggtgtc
    31501 actcaccctg aagccccgcg tgttttcccg tgaaatagac ctgcactcca cgtatcaacg
    31561 cttcgatgcc gcgattgatc agacccttct tctgaatgga acgcggacgt atgtgggcta
    31621 caactacacg tactacaatg gctttgcggc gggtgcctcc gtctcttcgt ccgcaataag
    31681 accgttcacg accgctgaca ttgcccccca gggcatgaag tggtcactcc ggtatgaccg
    31741 ctggtggaac tggtactttg aggatttcga cgcgggtacc gggcttctca acgaggtgta
    31801 cacaccctac cactacaatc aggtgtcgtg tgacgccgcc tggtacctgc ccacgccctg
    31861 gcgggaggaa cacaccgtgg gcgtggaagg gcgcgtcatg cgtattgacg ctgcggagga
    31921 cagcttcttc tatgagggta tcggtggcat tatcgggttg cgtggataca cctattacca
    31981 gctccagggc aaacaaaccg catgggggag gttgctgtac cgattcccgc tgctgacctc
    32041 catcgaccgg aaactggggc cggtctattt cgacaaaatc tacgggaccg tgtttgcgga
    32101 ggcaggccgc gtctggcgtg actcataccc ttcgtcgtgg acgcccggac tcaagcgtga
    32161 tgtcggcgca gagatccgcg ctgatctttt ttccttctac gggtacccct cccggttttc
    32221 cttttccgcc gcgcgtgcgc tggatgctgc gccacattct gacaggacca agttctacct
    32281 taccgtgttg ttcgggtatc tgtaacgggt tgccccccaa catgacataa ggcggattat
    32341 caacacctcc tgaacgaatg ccgacggcaa taatcctgcg aagacgtatg cctctcaagt
    32401 gagctcaccg gctcgcctcg ccgttttgtg cgctgacaca cacgtagcaa ggccgccgca
    32461 atggcgccgt gctttggagc ctgttcccgc ggtgggtaca tttttccaag gtaccggcgc
    32521 gaaggtgccg attttgtcgg cgtttttcgc gcgccatcaa ggaaacttct ccacaatccc
    32581 gcgctcgtcg tccacacaca ggcgagcagt ttattgaggc agcaggggag caactcatga
    32641 ccgaacatgg taacggaccc gaatcaggtc tggaaataga tccatcctta ctggatgcgg
    32701 atgcggtcat tcgcgcactt cacacggacc ctgaacacgg cctgagctca gcagaagctg
    32761 cggctcgcct tgcccgctac ggaaaaaacg aacttgtcgg cgcgccgccc gttcccgtct
    32821 ggcgcaaact cctggcgcag tttgaagacc cccttatcta ccttctgctg gcggcggtgg
    32881 cgatttcggc tgtcgcgtgg ttcatggagg gcgcgcacgg gtggcccgtg gattcagcag
    32941 taatccttgc catcgtagtc gccaacggcg ttctcggata cgttcaggag gcgaaggcgg
    33001 aaaacgcggt ggccgccctg gcgagtatgg cggcagcaac ctcggcagtt gtccgcaacg
    33061 gtcaggtgga gcgcattccc tcatctgaca tagtcccggg ggacgtgttg gtactgagtg
    33121 agggagacaa catcggcgcg gatggacggc tgttcaaaac ggcgaacctg cgcgttgcag
    33181 aggcgcctct tacaggcgag agcgagccgg ttcagaagga aacatcggca cttcaggaac
    33241 ccagcaacct tggtgaccgg cgcaatatgg tgttcaaagg cactgccgtg acacagggag
    33301 taggaagggc cgttgtcacg gcaaccggaa tgaacacaga agtcggcaag atcgcccgca
    33361 tgcttgaaac gacggaagac gaggcgactc ccctgcagaa ggaaatcgtg cggctgggaa
    33421 agatgctggg aatcgccgtc gttctgatcg ccgcagtcgt cgtcacgaca acgttcgtgg
    33481 tgttccgtgt cgacacaatg gacgaggctg tctcgctgct gttgcttggg gtttcacttg
    33541 ccgtggcggc ggtgccggaa gggttgccgg cggttctttc ggtggttctg gcaatcggcg
    33601 tgcagcgcat ggcggctcgc aacgcgattg tgaagaaact gtcttccgca gagacgctcg
    33661 gctccgcctc ggtgatatgt tcggacaaaa ccggcacgct gaccaaaagc gaaatgacag
    33721 tgacgaagat cgtcaccgct tccggagagg ttgagttgac cggggttggg tacgatcctt
    33781 ccggccgggc tgaagtcgcc gggcaggaaa ttggcgattc ggcgctgcgc acggaagcac
    33841 aatgcgtgct cgccggtggc agccttgctg gaaacgcatc tcttcgccag cgggacggtg
    33901 tctgggagat tcagggggac ccgaccgagg ctgcattcct cgtggcagca caaaagctgg
    33961 gtggcacgca gcagatcatg gagcgctatg aacgcctcgg ggagatccct ttcacctccg
    34021 aacgcaagcg catgtccgtt ctgttgcgcg aaaggaacac cgggcgcctg acgctgttct
    34081 ccaaaggagc cccggatgtg ttgctcgaac ggtgcacgca catccttgtg ggcaacgagg
    34141 tagcgccgct ggacgatgcg cgccaagcgg caagcgtggg gaatatcgaa gcactttccg
    34201 cgcaggcgtt cagaaccatc gccgtcgcct accggaatct ggaaggcgag ggagtgatcc
    34261 cggccgagga aggtgcgccg gtggatgagt cgcttgaggc cgatcttgtg tacgtgggaa
    34321 tcgtcggaat tctggatccc cctcgccctg aagcggcaaa agcggtggtg gaatcacacc
    34381 gtgcaggcgt gcgcgtcatc atgattacgg gagaccatcc caagacggcg acgcgcatcg
    34441 cctccgatct cggcattgtg gggaaggacg caaccgctct gacggggatg gagatagaca
    34501 aactggacga ggcagcgttg cgcgaagccg tgcgcacgac ctccgtgtat gcccgcgtgg
    34561 cgccggagca caaactccag atcgtggatg cgttgcaggc tggcgggaac gtcgtggcga
    34621 tgacgggcga cggcgtgaac gatgctccgg ccttgaaaag ggcggaaatc ggcgtagcga
    34681 tgggggttac cggaactgac gtgacaaaag aagccggcaa catggtgctt gcggatgaca
    34741 atttcgcgac aatcgttgcc gcggtgcacg aaggccgtgt gatcttcgac aacatccgca
    34801 agttcctgcg gtatctgctc agttccaaca tgggagaagt gctggcggtc tttttcgggg
    34861 tggtgctctc cacggtgata gggttgactt ccggcgacgg aggaatcgtg ttgccgcttc
    34921 tggcaacaca gcttctgtgg atcaacctcg ttacggatgc agcgcccgcg atggcgctgg
    34981 gagtagaccc ggaaacggag gacgtgatgg cccgcccgcc acgaaaactc acggacaggg
    35041 tgatcgacgg ccggatgtgg accacggtgt tgatcggcgg ctcggtgatg gctgccatga
    35101 cacttctcac cctggatatc cttcttcctg gcggtttatt cgaggggacg ggatcgctgg
    35161 acactgcgcg caccgccgca ttcacggtgc tggtgttcac ccagctcttc aacgtgttca
    35221 actcccggtc tgagcacgca agcgcatttc atcgtctgtg cgcaaaccga tggctgctcg
    35281 gcgcggtact gctcggagtg gtcctgcagg ttgccgtggt ccacattcca ttcatgaaca
    35341 cggcattcgg tactacaccg ttgacgggtg aacaatggat tctgtgcgtg gtgatggcgt
    35401 cggcggttct gtgggtggaa gaggcgcgaa agcttgtgtg gcgaacaaca aacctgaagg
    35461 ggaagaaatg acgttgccag aacgattccc acccataacc gccaaatgcc cgcatatcct
    35521 ccatggcgga gactacaacc ccgaccagtg gatgcacacg cccgaggtgt gggatgagga
    35581 catgcgcctc atgaggctcg caggctgcaa tgtcatgtct gtcggcattt tctcatgggc
    35641 ggcactcgaa ccgagggaag gccatttcga attcggctgg ctcgacacga tcatggacaa
    35701 gctcgcggcc aacggcgtgt atgttgtgct tgcgacgccc agcgggtcga agccgccgtg
    35761 gttgagcgag gcctaccctg aggtatgccg cgtgaacgcg caggggcaac gcgagccgca
    35821 tcgcgagcgc cacaaccatt gccggacgtc gcccgtgtat cgcgagaaat gccggatcat
    35881 caacacgcag cttgccacgc gctacaagga ccacccggcg ctggtcgtat ggcacgtctc
    35941 taacgaatac aacggtgggg actgccactg cccgctctgt tacgaagcct tccacacgtg
    36001 gctgaaaaaa cgttacggca ccctggagga actcaatcac gcctggtgga cgggtttctg
    36061 gagccacgct ttcaccgatt gggcgcagat caggcctgtg gaccccagca tacacgggct
    36121 catgctggac tggcagcgct tcaaaacaga ccatacgatc gacttcttcc ggaatgaaat
    36181 cgcgcctctg cgcgaattca ccccggacat ccccgtaacc accaacttca tggggttcag
    36241 cccgacgctg gactacgcga agttcgcgcg ggtggtggat gtcgtgagct gggacagcta
    36301 ccccgcctac catgaccgcc cgaacgactg gcttaacgcg gtcgaagtat cgctgaccca
    36361 cgacctgaac cgttccttca agaacaaacc tttcatgctc atggagtgct cccccagtgt
    36421 gcagaactgg aaaccggtga acaaactcaa acggccgggg ctgcacaggg tggaagcgct
    36481 ccagtgcatt gcccacggcg gcgacactgt gcagtacttc cagtggcgaa agggtcgcgg
    36541 cgggtgcgag aaattccacg gcgcggtggt tgaccactac ccgacggaac acacgcgggt
    36601 gttccgcgag gtggcggacc tcggcggcgg gctggcctcg ctggacgatg tggtgggaac
    36661 gataaccccg gccgaggttg ccattctcta cgactgggag aaccgctggg ctattgacca
    36721 gacggccggg ccacggaacg agcggaaaga ttaccagcct acctgcgtgg cgcactaccg
    36781 gcccttctgg tccgcgggcg tctcctgcga cgtggttaac gagg
//
LOCUS       DAOSOO010000010        34367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1287_length_34367_cov_5.456371, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000010 DAOSOO010000000
VERSION     DAOSOO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 34367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34367
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_34367_cov_5.456371"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLT86_01560"
     CDS             complement(<1..837)
                     /locus_tag="PLT86_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HOT35332.1"
                     /translation="MRSISLDGEWQLFPVPPTHPPITHPRELRSSGIPPIRGTVPGNV
                     ELDLLHAGVIPDPFFGTNVLRLKDFELHNWWYSRNFATPEFESGDRVVIRFEGVDTFA
                     TVWVNGNRAGSCDNMLIAHEFDITSLLRTGQDENELCVLLESPLTRAGSHRYEPSIAG
                     LPTNLEQAYVRKAPHMYGWDIMPRAVSAGLWRPVFLDVHRQPEIVDLFVRPYHVTADE
                     AAIQFRWQIQAEPTTWERCTVRVRGRCGASEFSHQESCRFIAGDFSVVVKKPQLWWPR
                     GYG"
     gene            1018..2583
                     /locus_tag="PLT86_01565"
     CDS             1018..2583
                     /locus_tag="PLT86_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOT35333.1"
                     /translation="MENVNQRPRRQILWVDDEIDLLRSHIIFLADRGYDVTPVTNGED
                     AVALASERSFDIVLLDEMMAGQDGLTTLDEIKSIHPTLPVVMVTKSEEEELMNEAIGK
                     RIDDYLVKPVNPSQIVLACKRLLDARQIREQKLVRDYVSEFSNLHTQISTAGWSEWMD
                     IFGRLAAWDLEIHSYNDPSLSEMHHSQWRHCNQVFARYVEENYAGWVAAPRTGPITSA
                     EVVRQFVVPHVGARPVYFIVIDCMRLDQWLAIEPLIAEYFAVRREHYYATLPTATPFS
                     RNALFAGMFPAGIARRFPDLWTPGNDEDQSLNRFENQLLVENLARVGVKQPVHYQKVL
                     DAGDGRAVLKKIGTWKNAGLVALVFNFVDHLSHGRSESEILQEITPDEASFRSLTVSW
                     FSRSALFDILKWISRTDSTVIVTTDHGSVLGTRATIARGNRETSTNLRYKSGTNLQCD
                     PKEALLVRDPLTFGLPRFTPSTNFIIAKEDFYFVYPTRANEFGRHYRNSFQHGGISLE
                     EMILPVVTLTPRR"
     gene            2591..3031
                     /gene="tsaE"
                     /locus_tag="PLT86_01570"
     CDS             2591..3031
                     /gene="tsaE"
                     /locus_tag="PLT86_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT35334.1"
                     /translation="MLIDPEPRTYVTTSPDETREFGKRIAPWLRPGSVVFLYGDLGSG
                     KTTLVQGICAGLEVGVWPNSPTFTLINQYEGTVPVFHCDFYRLGDPGELATLGLEEVF
                     FGDGIALVEWPELAEEWAPSHTIRLTITRRSAKKREIVLRGLSL"
     gene            3294..4421
                     /gene="dnaN"
                     /locus_tag="PLT86_01575"
     CDS             3294..4421
                     /gene="dnaN"
                     /locus_tag="PLT86_01575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT35335.1"
                     /translation="MRVQMDQSELLGMLQTVIDAVPSKSTLPILSMMLMVIEEGTCSL
                     SGTDLEVFLTTTRAVQDAEDGRITFPGRLMFDFVRELPPGPITISEADGRVTLKSANG
                     ECSLLGMPANEFPTVPKGIEGGVTISVDGDLLRRMIEKTAFAVHPDQTRPVLSSICWR
                     VNPNGMVMVATDGHRLAKISEVVEGIHGDPLQVIVAPRVLQQVERLIRDDNKLESITI
                     GERQIHFAYSGANLVTRLVEGKYVDVESVIPKDNNRRLIVSKEELQPAVRRMQILSSQ
                     QNHQVRLALHKDELELSTVNRDTGAEAHERISAQYDDEEMEIGYNANYLKEVLSKIDS
                     DQVEFHFREPVSAAIIKPAKQPEGEDYFCLLMPLRLLNTDS"
     gene            4471..5589
                     /gene="recF"
                     /locus_tag="PLT86_01580"
     CDS             4471..5589
                     /gene="recF"
                     /locus_tag="PLT86_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOT35336.1"
                     /translation="MHGIQAVHLTSVQLRDFRSYRSASAVFAPALNIVSGENGQGKTN
                     LLEAIRLISVGRSFRGATDGEMVSWGQPSGTITGTIGDGPVRQIVLTITPGERKRGTI
                     DGAALNGLAELVGVLRTVAVSSDEIEQQFRSQTGRRRMIDVMISQVDRRYLTELRRCK
                     TIVAQLNAVYRDPANDDALCDVLEHQLAEVSLRINRRREAFFRDFCGAVEGHFSRLFG
                     IGNLVLESRTTLPGDGDMSDLDRAVDVLRESRPSARRAGFAVRGAHRDRYRVLLDGKD
                     IESHASKGQVKIAYLAWKLAEGDFIERETTRSPVWLIDDPFSELDAKRAAALLASTEQ
                     RGQVILTNARTDDVAVLKNGPAHHLRVHDGIVEGVHGV"
     gene            5582..5962
                     /locus_tag="PLT86_01585"
     CDS             5582..5962
                     /locus_tag="PLT86_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOT35337.1"
                     /translation="MSDSAGNDQRAGTTLPLQTVLQRMAGQWQVRQASALEILKEHWD
                     EVTGEVWARTITPEMVDEKGRLVVTVRSAPGVAMEIQRKGRRWRQILEGTIRVSGYEF
                     TDVVLAPPYSLRKRTPRERTRGNE"
     gene            5955..7991
                     /gene="gyrB"
                     /locus_tag="PLT86_01590"
     CDS             5955..7991
                     /gene="gyrB"
                     /locus_tag="PLT86_01590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT35338.1"
                     /translation="MSEELHLNNHGIPENTPENANGHLSGKANGNGIGNGSTAVDTDT
                     AYDANSIQVLEGLEAVRKRPGMYIGSTGTQGLMNLIREIVDNSIDEALAGRCDRIEVV
                     LLPDGSARVSDNGAGIPTDIHPTEGVSAVEVVMTKLHAGGKFGHSAYKVSGGLHGVGA
                     SVVNALSEWCIVEVHQKGRAFRQRFERGTTVTPLEDVGEAQDTGTITTFKPDATIMTE
                     TEFAYEPLMHTLREQAFLNRGVTIVLRDERGEEVREDVFRFDGGIAEFVQFLDEGKTP
                     LHTPPIYIERMRDDVIVELAMQYNASYSEAVFTYVNNINTVEGGTHLAGLRAALTTSI
                     NAYAQANNLLKDMKEGLSGEDVREGLTAILSLKIPNPQFESQTKIRLGNPEIKGLVQS
                     VVTEGLNEYFDENPQVIKRIIMKSVEAAHARLAARKARELTRRKSILDGGGLPTKLSD
                     CSITDPAACEIYLVEGDSAGGSAKQARDRRYQAVLPMWGKMLNVEKARIDKVLGNDKL
                     QPVIQALGASIGEEFDLSKLRYHKVIIMADADVDGSHIRTLILTFFFRFMRPLIEAGH
                     VYIAQPPLFLVKRGKEEHWVFSDAERDAALARFENQKGVTVQRYKGLGEMSAEQLWST
                     TMNPETRTLLRVSMEDAVEADRIVTVLLGADVEPRKRYIEANAQYVKELDIHAA"
     gene            8116..10716
                     /gene="gyrA"
                     /locus_tag="PLT86_01595"
     CDS             8116..10716
                     /gene="gyrA"
                     /locus_tag="PLT86_01595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT35339.1"
                     /translation="MLESRERVIPIQIEEEIRESYMEYALSTLVARALPDVRDGLKPS
                     QRRILVAMNDLHLGPTAKYYKCAKVAGDTSGNYHPHGEAIVYPTLYRMAQPFNMRYRL
                     VDGQGNFGSIDGDPPAAMRYTECRMTSVATELLADLDRDTVDFQSNYDERLQEPKVLP
                     ARFPALLCNGASGIAVGMATNIPPHNVGEVVDALIAQIENPDIDVNGLMQHIKGPDFP
                     TGAIIYGIEGIREAYATGRGLIKVRARANIETLKNDRQNIVITEIPFQLNATTLKEAI
                     ASVARNRVVDGIADFRDESDRDGMRMVIELKRDAYAEVTLNQLFKHTPLEQTFGINLL
                     ALVNGQPKLLTLKEMLQHYIDHRREVVRRRTSFDLNQAQAQAHIVEGLLLALRDIDNI
                     IETIRKSRDAAEARGQLMSVFQLSEKQADAILDMNLRRLTSLEVSKLENQYADLTTQI
                     AEFQTILSSDANILAVVKKELLDVRKKHGDERRTEIVASASDFSIEDLIAEEDMVVTI
                     SHTGYIKRIPINTYRQQRRGGRGVTGMTTKEEDFVEHLFVASTHSYLLCLTEKGRCYR
                     LRVHEIPQGSRSSKGRPLVNVLEAAKDEIIAAVVPVRHFEEGRYLVMVTAKGTIKKTP
                     LSAFANINRLGVIAINVLEEDELIEAAVTDGTYDIILGTRNGYAIRFPEDTVRPMGRT
                     AAGVRGIRLRGDDRVVGMVVNKHHDRTLLTVCENGHGKRSPIEDYRITNRGGKGIINI
                     KATERNGAVVAIKEVIDEDELMIVTQNGILIRLPIADLSVIGRNTQGVRLIRIEEGDV
                     VQDVARVVVKNGDAGAASPEGEAEEEVEEDSEAPANGLPEEEYRDDAESREDEEDDGG
                     DHVDLADEEE"
     gene            10770..13382
                     /locus_tag="PLT86_01600"
     CDS             10770..13382
                     /locus_tag="PLT86_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT35340.1"
                     /translation="MALRVVFAAEHVADFADVLDEIRGRGYEVQLIHPFDYQPTASRS
                     SDQDAVAIFIPLDRGGVFRLGGYQSTDVTLPRSAATFLIGMAPETTSDGTQSHQRRAL
                     DGVIPYPPSLEDLLRVLSGCEARLVAGTPDSGSPPDNETDHSRLKEFYERIVDTISDG
                     IRVVDGRDRTIVLANRAHLAWIGKQAHEVIGVRARDVPWGFDANDCPVQMTDIVDAAI
                     ETAEPARRLIATCPHAGRELRWIDVVAYPMSGLSGSVDHVVLVGRDVSERVRMETSLA
                     AERDRRDHVLRSAPVGILTTDSTGTIEFANPPIAQIMGLEGTEAVVGKNILVLPQLQA
                     MEVAAGLWEVLTEGKPLHLDRVSCRTTGKREAICNVRAEPIISHAGDISGIVAVFEDV
                     TETVLLQRRLSATTADLAMLSEIGELFQDSRDTEVILQTILVGVTAGEGLGFNRAFLL
                     VADHETGTLRGSYAIGPSDLAEASRIWQELGKKPHSFRDILEGYREAISRDEVTVNKV
                     AKSLVISLEDAQNIASKAAKERRAFLVTDGMNHPLVPRQLAEQLGTDSFVVAPLIFDD
                     RVEGVIIADNAITRKSITELDLRSLELFAHQARLAVERSRLHAELERQLEELREAHEL
                     LRANHEEMLRTEKLAAMGQVAAQVAHEIRNPLVAIGGFARSIRNSLREDDQNRKFAEI
                     IVEETARLENILREVLDFARPPHPRRDRVDVGSVLRRATEMLERELQKRSIRINVQIP
                     RDPVMVLGDAGQLRQVFHNLIQNAMDAMERVDTSSRLLSISLSCETDRQVAVTVEDTG
                     GGFEADALEHVFEPFFTTKSHGTGLGLAICQQIVRDHGGTLSITNKPERGATFTVRVP
                     LLEKEVVNGENPGC"
     gene            13360..13713
                     /locus_tag="PLT86_01605"
     CDS             13360..13713
                     /locus_tag="PLT86_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35341.1"
                     /translation="MAKILVVDDENALRVLYKHELTQDGHDVDLASTADEAVAKVRET
                     AYDVVILDIRMPGKDGLEALTEMFAERRNIKVIINSAYSSYKENFISWAAEHYVVKSA
                     DTTELKEKIAQVLGG"
     gene            13710..14954
                     /gene="glgC"
                     /locus_tag="PLT86_01610"
     CDS             13710..14954
                     /gene="glgC"
                     /locus_tag="PLT86_01610"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT35342.1"
                     /translation="MNTTKTRFTPGALLRNTLTVILAGGHGTRLYPLTKHRAKPAVPF
                     AGAYRIIDFTLSNCVNSGLRRVFVLTQYKSFSLDRHLQQGWSSLRYDMGEFILQLPPQ
                     ERMKRSWYEGTADAIFQNLYVFQEQRPEYTVILSGDHVYLMDYAPMLAAHENTGADLT
                     IAAIPVPRQDARRFGVLQVDASNRIVGFEEKPRDPASWRDDQTSLVSMGVYVFSTDVL
                     VRRVLEDAKNPQSEHDFGQNIIPGMVNRDAVFAYSMPAMSGCEPYWRDIGTIDSYWQT
                     SMDILRPDPPLNLYDPSWPVRTYRDHRPPPIVIDDGHPAGPFIRNSMVCAGARITSAR
                     IDRSIVALDNVVCPGADIEETVLMSRVRVGKGARIRRAIVDKDVDIPAGETIGYDLER
                     ERKRFLVTDNGIVVIPRGYVFD"
     gene            14988..16016
                     /gene="galT"
                     /locus_tag="PLT86_01615"
     CDS             14988..16016
                     /gene="galT"
                     /locus_tag="PLT86_01615"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT35343.1"
                     /translation="MQELRKDPVTDRWVIVSTERGARPTDFESLPRPKAPQLCPFCYG
                     FESTTPPEIYCVREAGTAPNTPGWSVRVVPNKFPALRIEGNLVREGVGMFDKMTGIGA
                     HEVIIEHPDHQRRFWDFSVQDIERVLAAYLQRTNDLKRDIRLRHMLVFKNEGEQAGAS
                     VAHSHSQLIATPVVPKRVREKLDGVNRYLSYKERCVYCDEIREEQRFGERVVFENDSF
                     ISFCPFASRFPFEVTLLPKTHRPDFSAIDGTERTALAEALRVTMRKLNEALNGPQYNW
                     IFLAAPTRLSGRESWEDFHWHVEIIPRLTKIAGFEWGTGFYINPTPPEHAAAYLRGVA
                     GCGSPGLD"
     gene            16053..18194
                     /locus_tag="PLT86_01620"
     CDS             16053..18194
                     /locus_tag="PLT86_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOT35344.1"
                     /translation="MPVLNVALLWHMHQPDYRDPETGMLALPWVRLHATKGYYDMVKV
                     IERADTHTHACFNFTPSLVSQLQGYVDGTLTDKYLELSRRPASDLSAEERRFVASNFF
                     HANRRTMIDCYPRYSELRTMSRLPSGSVRDFSDQDLTDLVVWFNLTWCGYGARADFPF
                     LMELVRQGRGFSEEQKLQMLALQRDIIAEILPSYRKAAEGERIELTSTPFYHPILPLV
                     CTTDSAKAGLPDRELPAPPFVAPEDARHHVRRAIALHESVFGSRPDGMWPAEGSVSTE
                     AVQILAEEGVKWIASDQDVLFRSNPSLGQRDLYVPYSVGERGGQLSIVFRDKSLADKI
                     GFRYSGMTTDAAVNDFMDNLRAIWTGQPKSGGEQLVTIVLDGENAWEYYPDGGEAFLT
                     AMYSKVANSKDFRWTTISDYLARNPPRHAIRRIFPGSWINGNFDIWIGSSEENRAWEA
                     LRDTRSALISAQDRLSEKARQEAWEHIYIAEGSDWFWWYGDDFTTALQGEFDRLFRAH
                     LAAVFELINVPVPAWLVKPIRKGRELAASKPANLISPTLDGRSTSYYEWAGAGHFDAR
                     SADGTMAREAPLVSAIAFGADHHRWYLRIDWSRPLAPNERSELEVVCVFPNRPGTQVI
                     IGPFGFETRDIPVKIVEHGTEVQITPQAVFRDLVECAVPFLLLGAAPGTLVEFVVSVR
                     QGGDEIERWPRDGVLAFDVPTDTFELEHWTV"
     gene            18196..19164
                     /locus_tag="PLT86_01625"
     CDS             18196..19164
                     /locus_tag="PLT86_01625"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT35345.1"
                     /translation="MVLDPRTLRVKSIAYIREALANCGAVSDSVIRAMEEDDRAAVRR
                     MAEQLKKAPSRPHTDMLVHEQQCWQSGSVRVAGVDEAGRGPLAGPVYAAAVILPRDGI
                     PSGINDSKRLSARQREQLDPLIRACALAVHVASVGVEEIDRVNILQASLKAMRTAIAG
                     LGHPPPDHVLVDGNITPGSGLPETAIVDGDSKSVSIAAASILAKVARDRFMEEMDTRY
                     PGYGFARNKGYGTREHLEALARLGPCPIHRRSFQHVACASEYPTHSDLIAFFKRSLVV
                     SESLEELRSVAAIIAEKKNVLPPEEVPALRRLYRQQFKLLRSRCDT"
     gene            19161..19532
                     /locus_tag="PLT86_01630"
     CDS             19161..19532
                     /locus_tag="PLT86_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT35346.1"
                     /translation="MKRGRLGEELAAGYLKRAGWTLCARNWHAGRAGEIDIVGWDGDI
                     LVFVEVKTANTSRYGHPVTWVDARKQSQLARLAELFVAKGDFVFSAMRFDVITIDAEK
                     QPPAITHLVDAFRPDIVGETG"
     gene            complement(19569..20558)
                     /gene="dctP"
                     /locus_tag="PLT86_01635"
     CDS             complement(19569..20558)
                     /gene="dctP"
                     /locus_tag="PLT86_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOT35347.1"
                     /translation="MVTSLSTKLRVALLTAVLSGGLLSACAPRETEVLLRVRHGAPPA
                     MPMHRALLKVASILEERSRGRIHLVVEAGLSLPYQIDEMLKGSDEISMSGMGSLSDYY
                     PPLGALEAPYMFRDIDHFYHTVETPFWRSLVTEVERRSGLHVIDMWHQGVRQVTLRDQ
                     AARSPAEFGTIKLRVPPSQMYVECARVLGALPTTMNINDVRTAIRAGTVDGQENPLPT
                     IEAMHFHDVCSYLVLTNHSITPIMPIVSAAFWRTLSERDRLLITEAFHEGGRYNRQIV
                     EEEERELIHEFEQSGMIVIRPDLRLFRDRAEKVWRRYREVWGDSLVARIQAVR"
     gene            complement(20580..20936)
                     /locus_tag="PLT86_01640"
     CDS             complement(20580..20936)
                     /locus_tag="PLT86_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT35348.1"
                     /translation="MKTDEVKRNGVVTRVAGDVAILDILGDLTAQTREEVEAAYQHAT
                     ESGAKKLLLTFTKDAYINSGGIAIIINVAIAGRKHGQKIRIVEPSEHFQKIFAMVGLK
                     QYVEIYASESEALSGF"
     gene            complement(20999..21439)
                     /locus_tag="PLT86_01645"
     CDS             complement(20999..21439)
                     /locus_tag="PLT86_01645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35349.1"
                     /translation="MGSNAMTIELQVPSTAGYERVAMDAAASVARLVGFRQSRIEDLK
                     TAVAEACINAMEHAHKFNEAMKVVVALTVQDSTLRIDVVDQGSGMQNNPTDPDISRKF
                     SGEQAARGWGMFLIKALMDEVEFNVRSELGNVTRMIIRLEPPGK"
     gene            complement(21496..24585)
                     /locus_tag="PLT86_01650"
     CDS             complement(21496..24585)
                     /locus_tag="PLT86_01650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT35350.1"
                     /translation="MVCARLILIVLSAAASATARDPLLESSRWRFFEDPKEIASLYTN
                     DLVQEIGGGVWLTTDRGLALHDGTQFVKKEIPGIGPSARFHNLLYSADGTLWIGTSEG
                     LAKYIAHQWHYPVQQGNITALAESADGAIWVGASPAEDGQFARDKRGAAPRPRTTVSV
                     YERRSWREFTSADGTLPSLPVRVLFGDQDGSVWAIFGDNNGHSEIYVYSGATWRNVAR
                     EIGLPSVEYLSLAQTRDGAIWFGTAGHGVLRWRAGSLERFVGDRFASSFTAASLLPMP
                     DGSLWAVGSPAGTLLEFRDGAWIGHSVRQVGVTGRLVSKIVYALDDAFWFVIPGRGVA
                     RFDRRGGPWWSYDAGDGLPESARVSCFARTGDGIQWVGTTHGLLLFDGKRFSDDRVPA
                     ELRGKPVSALLSFPDGRLWVAIASPVPPPTIWERSPRHIASQWRRIPPFLPSGGKSTI
                     HAFLRSRNGDVWAGVSGDVKARGVFRFRNGSWERLSGSQGAGTDDVLALAEDTAGGIW
                     AGMRNGLSRFDGVRWSAFDGQNSVRFEHISALCAARDGSVWVGGNSPNAGVSRYAGGR
                     WTHFTVDSGLASDDVWSIVEDEYRTIWVGTAGGVSRYFRGQWLTFRKESGLAEDHVSA
                     STLLGDGSLLFGHFDGAASRYHATSSDPPRSFIEPLPERVAHPGFLTVRWTGSDRFGR
                     TPAKDLLYSWRLDAGEPSPFSPATAHTFEELPAGEHVFTVRTLDQEGNDDGTGVSATF
                     AVEPPFWRTPWFVVPTVVAAVGIGFATAIAYQRNRRWKRAQAQLLSELQRELQVAQKL
                     QNALLPSVDPAVPGFEITGICRAASRVGGDYFAYVWDGEDRSKFGVIVADVTGHAMQA
                     AIPAVLFSGMVTAAARQTTQPAELLELLNATFWGRASRNTYICCTVALFDGERGILQI
                     ANAGGLDPLHRSGDRVTAISVEGERLPLGVAENTRFEQCEKKLGIGDIVVLVSDGVVE
                     ARSPAGEMFGFGRAEEAVRRGSTAFEVRDSIVTEVTAHMGEHEQDDDITIIAVRVTG"
     gene            complement(24716..25339)
                     /gene="rsmG"
                     /locus_tag="PLT86_01655"
     CDS             complement(24716..25339)
                     /gene="rsmG"
                     /locus_tag="PLT86_01655"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT35351.1"
                     /translation="MKSFERHLTDVQRKQLLGLVRLVHESNTRLNLVAPGDIPKLWER
                     HVCDSLQVLDFVDLCGRNVVDIGSGAGFPGLPVAIAVPSARITCVERIRKKAAFIRRA
                     TLELELHNVEVAWADSTELAQNSRYAGCVDIVTIRAVAHTRESLSLASGFLAENGFAL
                     LWQTRDQMLREPLPAGWMDEWFPTETDEGGARGIRKAFRATASSSLG"
     gene            complement(25336..27153)
                     /gene="mnmG"
                     /locus_tag="PLT86_01660"
     CDS             complement(25336..27153)
                     /gene="mnmG"
                     /locus_tag="PLT86_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT35352.1"
                     /translation="MAAARLGFDVVLMTMDVGKIGEMSCNPAIGGTAKGHVVREIDAL
                     GGVMGKLIDATGIQFKMLNRSKGPAMWSPRAQADRAAYRAAAQALVSSHERVRIVAEQ
                     AGQLRIEGGRIVGVIGVSGTEISCRAAVVATGTFLNGLMHVGLSSSVGGRVGEPAASE
                     LPRSLAAGGLALGRLKTGTPPRVDGRTINYSVMTPQYGDEPPPFFSFETPPRSVQQVP
                     CWLTWTNAATHRLILGGLDRSPLYTGRIKGTGPRYCPSIEDKVVRFPEKDHHQIFIEP
                     EGTATSEVYVNGFPSSLPEDVQLAALRTIRGMEEAVVNRFGYAVEYDFVFPHQLTPSL
                     EAKAVKGLFLAGQINGTSGYEEAAGQGFIAGVNAARSVSADPPIVLRRHEAYIGVMID
                     DLVTKGTEDPYRLFTSRAEHRLLLRQDNADVRLADRAREIGLIRGDELRRRQEKARAV
                     ESLVSRLREIPVPPEKANPVLTGKGTTPITEAQSAYRLLKRPEITLDEIVRMVSEDMA
                     GIHGEIAWQAEIEVKYEGYLVRQREAAERQAAAENREIPPDFSYDAVKALSAEARQKL
                     RTVRPATLGQAARVPGITPADITVIAIALEARRRRTEVP"
     gene            27425..28426
                     /gene="pdxA"
                     /locus_tag="PLT86_01665"
     CDS             27425..28426
                     /gene="pdxA"
                     /locus_tag="PLT86_01665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOT35353.1"
                     /translation="MKMRKPRLAVTMGDPAGVGPELCLTVATDTRATGAADLLFVGTE
                     SILQSVAKTLGRPLPDPAHICSVAEPKVAVPPGAVSAEAGRLSALYLDRAIVLAQKGE
                     VDGIVTAPISKEAWHLAGVPFPGHTEFLAAKTGTKEYGMMLVHGTWRVLHVSTHCSLR
                     DAISRVTQERILRMLLLFDGVLRDLGIASPRIGVAGLNPHAGEAGLFGDEEREHILPA
                     VLEARSRGVEVLGPIPPDTVFARGRLGEYDGVLAMYHDQGHIAVKTLAFEPSKDGGWA
                     SVHGVNVTVGLPIVRTSVDHGVAFDIAGKGIARTESMLDAIQLAARMALVRAGGSVR"
     gene            28423..29208
                     /locus_tag="PLT86_01670"
     CDS             28423..29208
                     /locus_tag="PLT86_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT35354.1"
                     /translation="MTPDDRPAVTGSTEANTGAKGIQRFLTLPTAAATGAFLALLLIF
                     VLGNWVVMPLITRGTDVVVPDVRGMDVADAKRLVEEQGLVFVNDSSDYATDESIPVDH
                     IVMQSPAPDVSVKRGRSVRVTVSRGAPKYVVPNVRGISLNDARVRLEQARFRVGNVSY
                     VLRTNEDLSEPYVQDQFPDAGTSLQKDAQVRLVVSIAPEMPALVGRDLGEASRTLAAM
                     GIQIGETTSEPDETAAPGTVLKQSIAPGTRVFASDRVDLVVTE"
     gene            29229..30284
                     /locus_tag="PLT86_01675"
     CDS             29229..30284
                     /locus_tag="PLT86_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35355.1"
                     /translation="MRIILRDLSYRYNGEAGVTNLNLQVESGELFGLLGPSGCGKTTT
                     LRLLAGFIQPEHGHVFFGDEDITGKRPEQRNVGMVFQSYALFPHMTVHENVEFGLLAR
                     GVPRKERGRRIRNALELVQLAPMERRSVADLSGGQQQRVAVARALVIEPTVLLLDEPL
                     SNLDARLREETGQELRALQRRLGITTVYVTHDRAEAFALCDRIGIMQHGRLIQVGAPQ
                     DLFASPGSLAVAQFIGHTNILPVEHIQEAGNGWTAQTGPHTLFGTRTLPQEPTAALAV
                     RPHEMTLDTTPISSKNTLLMRVSGRRFLGADMEISVQDGNLRLKILQRFSQSRVPEED
                     SQVYVHIPPEALIPLAR"
     gene            30317..32239
                     /locus_tag="PLT86_01680"
     CDS             30317..32239
                     /locus_tag="PLT86_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35356.1"
                     /translation="MPSSGLIEKTYRQTLNQAREIVSERLSALVRATGGDCEDAAEDI
                     AQSIVGTEQVVRLILSTPYDRSLYRPVLLEAADEVGRTFFPEISKRFFSPLSDAPAVE
                     SNAAEPERSLDSPQLEQAALALPPHLRSYFKKLLENAAERLPTARLSAIATDLLAERL
                     ERLQRHFPDGSIPPLSNAPVTDRMSDDEIILHARYVAARLAAAFPWGFFSKDTRRTGI
                     AVNYLVMREMRKTPEEALQEDVLSYIAVGLGPALRRCGGSVNRLLALGFPERIKPWMG
                     SHVPTGYWEETANRRDAVKWLVEQRLGIPPEAIGRAVHQNIISKKAFREAGLTWLLKN
                     VYGWSVAHALAESYPQLRPWECFCRVPVETWHGDAGRLLSSEAIGWAFREEGMQVAMF
                     RTQDAARVIKSTLNRWHLGAAFRIGFSENSFALLNSLFPNTFELWEVTHVPREAWKDP
                     ALRTNAVQWLLKEYHVEVEKIPVAIRNGLLSPDVFKMRGLDGLLRQMGSVWRVIDDVF
                     PGRFARWELGSVPNSYWRARSSVREAAIWALSRLGIELARVEDALRSGTLASRALTEM
                     GLGSLVNTLFRGDVRSLMQVAGVLPSARRVPLRRFYRQLRQDLVASHASEVEDRVQAL
                     RARQKERRRRTEEHWC"
     gene            32264..33400
                     /gene="bshA"
                     /locus_tag="PLT86_01685"
     CDS             32264..33400
                     /gene="bshA"
                     /locus_tag="PLT86_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOT35357.1"
                     /translation="MRIGITCHHTYGGSGTVATELGMALAKRGHQVHFVTFAEPYRLQ
                     FDPNIYLHQVDVMHYPLFDHPPTTLSLAAKMAETICTEKLDLLHVHYAIPHATAAYLA
                     LKLCPDHNVRLVTTLHGTDITLVGQDPSYFSITRFSIEQSDVVTAVSQWLKDQTLSRF
                     NVDHPIEVIYNSVDTERFRRVSVPACQRARYAPGGEPILVHISNFRPLKRVTDVIRAF
                     ALVRKQMPAKLLMIGDGPHRMPAQEVALELGVMGDVWFLGKQDRVELFLSLADLFLLP
                     SEHESFGLAALEAMACEVPVIASRAGGLVEVIEDGVSGHLVEVGDTEMMAELATRVLK
                     DQELAARIRAAALDRVHRLFSADKIVAEYEQLYRTALSSPKHQS"
     gene            complement(33471..34364)
                     /locus_tag="PLT86_01690"
     CDS             complement(33471..34364)
                     /locus_tag="PLT86_01690"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT35358.1"
                     /translation="MSKPRLGFIGLGIMGRPMVRNLLKAGYQVTVWNRSEPGIAACVK
                     EGAQKAASPADVTSRCDIVLSCVTDSPDVRQVYLGPNGVLDTIRPGMIAIDMSTISPA
                     VAREVASALEAKGAKMLDAPVSGGEKGAIEATLSIMVGGDAETVATATPILETLGKRI
                     IHIGPNGMGQTVKLCNQIVGALNDLAMCEGLILAAKSGADVSKMVDAVSAGAAGSWAM
                     NNLAPKILKRDFSPGFMVKLQQKDLRLVLDTAKELQIALPGTALVNQLFQSLEGKGCG
                     DEGIQALVKALEQMDGAEVRG"
BASE COUNT         7408 a         9986 c        10088 g         6885 t
ORIGIN      
        1 gccgtacccc cggggccacc atagttgagg tttttttacg actaccgaga aatccccggc
       61 aatgaatctg cacgattcct gatgtgagaa ctccgaagca ccgcatctgc cgcgcacccg
      121 taccgtacaa cgctcccagg tcgtcggttc ggcctgtatc tgccaccgga actggattgc
      181 cgcttcatca gcagttacgt ggtacggacg gacgaacaga tcgacgatct ccggctggcg
      241 gtgcacgtca aggaacacag gccgccacag gcctgcggaa accgcgcgcg gcatgatgtc
      301 ccacccgtac atatggggag ctttgcgcac gtatgcctgt tccaggttcg tgggaaggcc
      361 cgcaatggag ggttcgtatc ggtgtgaccc ggcacgtgta agaggcgatt ccagcaaaac
      421 gcagagctcg ttctcatcct gtcccgtgcg cagcagagag gttatgtcga attcgtgtgc
      481 tatcaacatg ttatcgcaac tgccggcccg gtttccattc acccataccg ttgcgaacgt
      541 gtcaactcct tcaaaacgga ttaccacacg gtcgccggat tcgaactccg gcgtcgcgaa
      601 gtttctggaa taccaccagt tgtgcagttc gaagtctttc aggcgaagta cgttcgtgcc
      661 gaaaaaggga tcaggtatga cgcctgcatg cagcaaatcc agctcaacat tgccggggac
      721 ggttccacga attggcggta tcccggagga cctcagttcc cggggatgag tgatcggcgg
      781 atgagttggt ggaacgggaa aaagctgcca ttccccatcg agactgatgc tcctcatggt
      841 aacctcgaaa aagcatggta ggaaacgatt ccgggctctg agtgttgaaa ggataacggc
      901 ggcggcatgc tgttcaaaac agcacccgcg gcgctgtcgt ccggtgcgtc cacaagggta
      961 tatttcccta cgtaacttct cggaatgcat caccacacgg tgtcggaggt acgccagttg
     1021 gagaacgtaa atcagcgccc acggcggcag attctctggg tggacgatga aatcgatctc
     1081 ctccgttcac acatcatttt ccttgccgat cgcgggtacg acgtaacacc ggtgaccaac
     1141 ggcgaagacg cggtggcgct cgcctcggaa cgaagctttg acatcgttct gttggacgag
     1201 atgatggcag ggcaggatgg tttgacaacg ctggacgaga taaaaagcat tcaccccact
     1261 ctcccggtcg tgatggtgac aaaaagtgaa gaagaagagc tgatgaacga agccattggc
     1321 aagcgcatag acgactatct cgtcaagccg gtgaatccca gtcagattgt cctcgcgtgc
     1381 aaaaggctcc ttgacgcgag gcagatccgc gaacagaagc tcgtgcgcga ttacgtgtcc
     1441 gagttttcca accttcacac gcaaatcagc acggccggct ggtcagagtg gatggacatc
     1501 ttcgggcgcc tcgctgcgtg ggacctcgaa atccattctt acaacgatcc ctccctcagc
     1561 gagatgcacc acagccagtg gcgccactgc aaccaggtat ttgcgcggta cgttgaggag
     1621 aattacgccg ggtgggtggc cgcgccgcgt acggggccga tcacatcggc tgaagtggta
     1681 cggcagttcg tggttcccca tgtcggcgct cgtccggtgt atttcattgt gatcgattgc
     1741 atgcgactcg accagtggct cgccatcgag ccactgattg cggagtattt tgccgtgcgg
     1801 cgcgagcact attacgctac actcccaacc gcgactccgt tctcacgcaa cgcgttgttt
     1861 gccggaatgt ttcccgcggg cattgcgaga cgattccctg acctgtggac gccaggaaac
     1921 gacgaggatc agtcgctgaa cagattcgag aatcagttgc tcgttgagaa cctcgcacgc
     1981 gtcggtgtca aacaacccgt ccattatcaa aaagtgcttg acgccggtga cggtcgggcg
     2041 gttctgaaga agatcggaac atggaaaaac gcggggctgg ttgcgctggt tttcaacttt
     2101 gttgaccacc tctcccacgg gcggtcagaa tcggagatac ttcaggaaat tactcccgat
     2161 gaggcttcat ttcgctctct gaccgtgtcc tggttctccc gttcggcgct gtttgacata
     2221 ctgaaatgga tttcccgcac cgattcgacg gtgatcgtga cgacggacca cgggagcgtt
     2281 ctgggaacga gagcgacgat cgcgcggggc aaccgggaaa caagcaccaa cctccgttac
     2341 aaatcgggca caaacctcca gtgcgacccg aaagaagcgt tacttgttcg ggaccccctc
     2401 acgttcgggt tgccacgttt caccccgagc accaacttca tcatcgcgaa ggaagatttc
     2461 tacttcgtct acccgacacg tgccaacgag ttcggccgcc attatcggaa cagcttccag
     2521 cacgggggca tttcgctgga agaaatgatc ttgccggtcg ttactctcac acctcgccgc
     2581 tgaacgcctc atgttgattg accccgaacc tcggacctac gtaaccacca gcccggacga
     2641 aacgcgggaa ttcggcaaaa ggattgcgcc ctggctcagg ccgggaagcg tcgtttttct
     2701 ctacggcgat ctggggagcg gaaaaacgac gcttgttcag ggaatctgcg ccgggctcga
     2761 ggtgggcgtc tggccaaaca gcccgacctt cacgttgatc aatcagtatg agggcacggt
     2821 gccggtgttc cactgcgatt tctaccggct gggtgatccg ggcgaactgg cgacactcgg
     2881 gctggaggag gtatttttcg gcgacggcat agccctggtg gaatggccgg agctggccga
     2941 agaatgggca ccatcccaca ctattcgcct gacaatcaca cgacgaagcg cgaagaaacg
     3001 ggaaatagta ctccgaggcc tgtccctgta ggctcggcac aggtatgacg cgttactgaa
     3061 gcaccgtgtg cagcgctctg gctgttccca acccggagaa acgcgcaacg cgaaatttcc
     3121 cgctgagcgc gccgccttct gcgagggtaa attcaaacgg ccgactgaca caccggtgat
     3181 gcgtcggaaa tccccacggg aagcatcaaa ctgcgttctg cggcacgttg gaaatctcca
     3241 tgggtttgcg tatcttttgt taattggttt tttcgggaaa ggaagggctt tctatgcgcg
     3301 tccagatgga tcaaagtgaa ctgcttggca tgcttcagac cgtgattgat gccgttccga
     3361 gcaaaagcac gttgccaatc ctctccatga tgctgatggt catcgaggaa ggaacgtgtt
     3421 cgctttcggg aaccgacctt gaggtattcc tcaccacaac ccgagcagtc caggacgcag
     3481 aagatggccg gattaccttt cccggccggt tgatgtttga tttcgtgcga gaactcccgc
     3541 ccggccccat tacgatcagc gaggcagacg gccgggttac gctcaaatcg gcgaatggcg
     3601 aatgcagcct gctgggcatg cctgccaacg agttccccac ggttccgaag ggtatcgaag
     3661 ggggcgtaac catctctgtc gacggcgacc tgctgaggcg tatgatcgag aagaccgcat
     3721 tcgccgtgca tcccgatcaa acaagacccg tgttgagcag catctgctgg cgtgtcaatc
     3781 cgaacgggat ggtgatggtt gcgaccgacg gtcacaggct cgcgaagata agcgaggtgg
     3841 ttgaaggcat ccacggagat ccgcttcagg tcatcgtcgc gccgagggtg ctccagcagg
     3901 tagaacggct gattcgtgac gacaacaaac tggaaagcat tacgataggg gagcggcaga
     3961 tacatttcgc gtactccggc gcgaaccttg tgacaaggct ggtggaaggt aagtacgtcg
     4021 atgtggagtc ggtgattccg aaggacaaca accggcgcct gatcgtgtcc aaggaagaac
     4081 tccaaccggc cgttcgccgc atgcagatat tgtccagtca gcaaaatcat caggtgcgtc
     4141 tcgcacttca caaagatgag ctcgaactct ccacggtaaa ccgtgatacg ggtgcggaag
     4201 cccacgaaag aatttctgca caatacgacg acgaggaaat ggaaatcggg tacaatgcga
     4261 attacctgaa agaggtgctg tccaagatcg attccgatca ggttgaattc catttccgcg
     4321 aaccggtttc cgctgcgatc ataaaacccg ccaagcaacc ggaaggcgag gattacttct
     4381 gcttgctcat gccgttgaga ctgctcaaca ctgattcgta gcacgcgacc gcacgctatc
     4441 ccgccgggcg cggaaacgga ttcaaaaagc gtgcatggga ttcaggcggt gcatctcacg
     4501 tccgtccagt tgcgcgattt cagaagttac cgttcagcgt cggcggtgtt cgctccggcg
     4561 ctgaacatcg tttcagggga aaatggtcag ggaaaaacga accttctcga ggcaatacgc
     4621 ctcatctctg tcgggcggtc ctttcgcggc gccactgacg gggagatggt atcgtggggg
     4681 cagccatccg gtacgataac cggcaccatc ggcgatgggc ccgtgcgtca gatagttctt
     4741 accatcaccc cgggagaacg gaagcgaggt acgatagacg gtgctgcgtt gaacggactg
     4801 gcggaactgg tgggcgtgct ccgcaccgtg gcagtatcct ctgacgagat tgaacagcag
     4861 tttcggtcgc agaccggccg gcgccggatg atcgacgtca tgatttcaca ggtggatcgc
     4921 cggtacctga cggagttgcg ccggtgcaaa acgatcgtcg cacaactgaa cgcggtgtat
     4981 cgagacccgg ccaatgatga tgcgttgtgt gacgtattgg agcaccagct ggcggaagta
     5041 tcgctgcgca tcaacaggcg aagagaagcg ttttttcgcg atttctgcgg cgccgttgaa
     5101 gggcattttt cccgtttgtt cgggatcggc aatctcgtgc tggaatcgcg caccacgctc
     5161 ccgggtgatg gggacatgag tgatctggac cgcgcagtgg atgtgttgcg cgaatcccgt
     5221 ccctcagccc ggcgtgcggg atttgcagtg cgcggcgcgc atcgggaccg ttaccgggtt
     5281 ctgctcgacg ggaaagatat cgaatcgcac gcatcgaagg ggcaggtcaa gatcgcgtac
     5341 ctcgcgtgga aactcgctga gggagacttc atcgaacgcg agacgacgcg ctcgcccgtg
     5401 tggttgattg acgacccttt cagcgagctc gacgcgaagc gggcggcggc gttgcttgcc
     5461 tcgacagaac agcgcgggca ggtcattctg acaaatgccc ggaccgatga cgtggcagtg
     5521 ctgaaaaacg ggcccgccca ccatttgcgg gttcacgatg gcatcgttga aggcgtccat
     5581 ggtgtctgat tcagctggca acgaccagag ggccggcaca actctgccgt tgcagaccgt
     5641 tctgcagcgc atggcggggc agtggcaggt gcgccaggcc tcggcgctgg agatactcaa
     5701 ggaacactgg gacgaagtta ccggggaagt gtgggcacgc acgatcaccc cggaaatggt
     5761 agacgaaaag ggacggctgg tggtgacggt cagatccgcc cccggagtag cgatggaaat
     5821 tcagaggaaa ggccgccggt ggcggcaaat cctggaaggc actatccggg taagcggata
     5881 cgagttcacc gacgtggtat tggctccgcc atattcgctg cgaaagcgaa ccccgcggga
     5941 aaggaccaga gggaatgagt gaagagttgc acctgaacaa ccatggaata cccgagaaca
     6001 cccccgaaaa cgccaacggg catctgtccg gcaaagccaa cgggaacggg atcggcaacg
     6061 gctcgaccgc ggttgacact gatactgcct acgacgcaaa cagcattcaa gtgctcgagg
     6121 gtctggaagc agtccgcaag cgtccgggga tgtacatcgg ctccaccggc acacaggggt
     6181 tgatgaacct catccgcgag attgtggaca actcaatcga tgaagcactg gcgggaaggt
     6241 gcgaccgcat cgaagtcgtc cttctgcccg acggatccgc gcgtgtttcg gacaacggag
     6301 ccggcattcc aaccgatatt catccgacgg aaggggtatc cgccgtcgag gttgtaatga
     6361 cgaagctcca cgcgggaggc aaattcggcc attcggctta caaggtctcc ggcggactcc
     6421 atggtgtcgg tgcgagcgtg gtcaatgcgc tttcagagtg gtgcatcgtg gaagtgcacc
     6481 agaagggacg cgcatttcgg cagcggttcg aacgtggcac tacggtaacg ccgttggaag
     6541 acgtcggaga ggcgcaggat accgggacca tcaccacgtt caagcctgac gccacaatca
     6601 tgacggagac ggaattcgcg tacgagccgc tcatgcatac tcttcgggaa caggcgtttc
     6661 tgaacagagg cgttaccatc gtcctgcgcg acgaacgcgg tgaggaagtc agggaggatg
     6721 tgttccggtt cgacggtggc atcgccgaat tcgttcaatt tctcgacgag gggaaaacgc
     6781 ccctccatac acccccgatc tacatcgagc gcatgcgcga cgacgtgatt gtggaactcg
     6841 cgatgcaata caacgcatcg tacagcgagg ccgttttcac gtatgtgaac aacatcaaca
     6901 cggtggaggg cggaacccat ctcgcaggat tgagagcggc gctcaccacg tcgataaatg
     6961 cctacgcaca ggcaaacaac cttctgaaag acatgaagga aggcctgtcg ggagaggatg
     7021 ttcgagaggg tctgacggcg atcctgagcc tgaagattcc gaaccctcag tttgaaagcc
     7081 agacaaaaat ccgcctgggg aatcccgaga tcaaggggtt ggtgcagtca gtggttaccg
     7141 aagggttgaa cgagtatttc gacgagaacc ctcaggtgat caagcggatc atcatgaagt
     7201 ccgtggaggc ggcgcacgcc aggctggccg cccgcaaggc gcgcgagttg acgcgccgga
     7261 aaagcattct ggacggcggc ggccttccga ccaaactgtc cgactgctcc attaccgacc
     7321 ctgcggcctg cgagatttac ctcgtcgagg gcgattccgc agggggatcc gcaaaacagg
     7381 cgcgagaccg tcggtaccaa gccgtgcttc ccatgtgggg caaaatgctc aatgtggaga
     7441 aagcgcgaat tgataaggtg cttggcaacg acaagctgca accggtaatt caggctctgg
     7501 gcgcaagcat cggtgaagag ttcgacctgt cgaagctgcg ctaccacaaa gtaatcatca
     7561 tggccgatgc ggacgtcgat ggctcgcaca tccgcaccct gatcctgacg ttcttcttcc
     7621 ggttcatgcg cccgcttatc gaagcgggac acgtatatat cgctcaacca cctcttttcc
     7681 tcgtcaaacg aggaaaggag gagcactggg tgttcagcga cgccgagcga gacgctgcgc
     7741 tcgcgcgttt cgagaaccag aaaggcgtca cagtgcaacg gtacaaaggt ctgggtgaga
     7801 tgagcgcgga acagctctgg agcacgacca tgaacccgga gacgcgaact ctgctgcgag
     7861 tctccatgga agatgcggtg gaagcagatc gcatcgtgac ggtcctgctg ggtgccgatg
     7921 tggaacccag aaaacggtac atcgaggcga acgcacagta cgtgaaagag cttgacatac
     7981 acgccgcctg acggcgccgc cgatcagttg ttcatcaccc gggcggacgc cgtcgtgcat
     8041 gtgcggacaa agctttccac gctttgtccg tactcggtgg ttgagtaaaa acgagcacaa
     8101 tttgagggag tttccatgct cgaatctcga gaacgagtca ttccgattca gattgaagaa
     8161 gaaatccgcg aatcgtatat ggagtatgcg ctcagcacgc tggtcgcgcg agcgttgccg
     8221 gacgtgcgcg acggtttgaa gccgtctcag cgacgcattc tcgtcgcaat gaacgacctc
     8281 cacctcgggc caacggccaa gtattacaaa tgcgcgaagg tggcgggcga cacgtcgggg
     8341 aattaccatc cgcacggtga agcgatcgtg tatccgacgc tatatcggat ggcacagccc
     8401 ttcaacatgc gctaccggct ggttgacgga cagggcaact tcggttccat agatggcgac
     8461 cccccggcgg ccatgcggta cactgagtgc cgcatgacgt ccgtcgccac agaactgctc
     8521 gcggatctgg atcgggacac cgtagacttc cagtccaact acgacgaacg gcttcaggag
     8581 ccgaaggttc ttcccgctcg gttcccggcc ctgttgtgca atggcgcctc cggcatcgcc
     8641 gtgggcatgg ccaccaacat tccgccccac aacgtgggcg aagtggtaga tgcgctgatc
     8701 gcgcagatcg agaacccgga tattgacgta aacggcctga tgcagcacat caagggaccg
     8761 gatttcccca ccggggcgat catttacggt atcgaaggga ttcgggaggc ttatgccacc
     8821 ggacgaggcc tgatcaaagt ccgggcacgc gccaacatcg agactctcaa aaacgatcga
     8881 cagaacatcg tcattacgga gataccgttc cagctcaatg ctacaactct gaaagaggcc
     8941 atcgcctccg tcgctcggaa tcgagttgta gacggaatcg ccgatttccg agatgagtcc
     9001 gaccgcgatg gcatgcgcat ggtaatcgag ctcaagcgtg atgcgtacgc agaggttacg
     9061 ctgaaccagc tcttcaagca cacgccactg gaacagacgt tcggaatcaa cctcctcgcg
     9121 ctggtcaacg ggcaaccgaa gctcctgaca ctgaaggaga tgttgcagca ctacatcgat
     9181 catcgtcgcg aggtcgtccg aaggcggacc agcttcgacc tcaatcaggc gcaggcgcag
     9241 gcgcacatcg ttgagggact gcttctggcg ctgcgggaca ttgacaatat catcgagacc
     9301 atccgaaaat cgcgtgatgc cgctgaggcc cgcgggcagt tgatgagcgt cttccagctt
     9361 tctgagaagc aggctgacgc gatcctcgat atgaaccttc ggcgcctcac cagtctggaa
     9421 gtgagcaaac tggagaatca gtacgcggac ctcaccacac aaatcgccga gttccagacc
     9481 atcctgagtt cagacgcgaa catccttgcc gtggtgaaaa aagagctgct cgatgttcgc
     9541 aagaagcacg gcgacgagcg acgcacggag atcgtggctt cggcatccga tttttccatc
     9601 gaagatctga ttgccgaaga agacatggta gtcacgatca gccataccgg atacatcaaa
     9661 cggataccga tcaacaccta ccggcaacag cgtcgggggg gccgtggcgt aaccggtatg
     9721 acgacgaaag aggaggattt tgtcgagcat ctgtttgtcg cgtcgacaca cagttacctc
     9781 ctctgtctca cggagaaagg acgctgctac cgccttcgag tgcatgagat accgcagggg
     9841 agccgttcca gcaaaggaag gccgcttgtc aacgttctcg aggctgcaaa ggacgaaatc
     9901 atcgcggcag tggtgccggt ccggcatttc gaagaaggcc gctacctcgt gatggtaacg
     9961 gcgaaaggga caatcaagaa gacaccactc tccgcgttcg caaatatcaa tcgcctcggc
    10021 gttatcgcga tcaacgtgct ggaagaagac gaactgatcg aagcggccgt aacggacggg
    10081 acgtacgaca tcattctcgg caccaggaat gggtacgcca tccggttccc ggaggacaca
    10141 gttcgcccca tgggcagaac cgccgctgga gtgcggggca tccgactccg gggtgacgac
    10201 agagtggtcg gtatggtggt gaacaagcac catgatcgca ctctgttgac tgtgtgcgaa
    10261 aacgggcacg ggaagcgctc ccccatcgag gactatcgaa tcacaaaccg tggcggtaag
    10321 ggcatcatca acatcaaggc aacggaacgg aacggcgcgg ttgtggctat caaggaagtg
    10381 atagacgagg acgagttgat gattgtcacc cagaacggga ttctgatccg cctcccaatc
    10441 gccgacttgt cggttattgg acggaacacg cagggtgttc gtctgatcag aatcgaggag
    10501 ggtgatgttg ttcaggatgt ggcgcgcgtt gtggtaaaga acggcgacgc gggcgcagca
    10561 tctcctgagg gtgaagcgga agaagaggtg gaagaggact cagaagcccc agcgaatggc
    10621 ctccctgaag aggaatacag ggacgacgcc gaatcgcgtg aagatgagga ggacgacggt
    10681 ggcgatcacg ttgacctcgc agacgaagag gagtagcttt aggcgggttc cacgcgtgaa
    10741 aaccttacct gtttctgcga tggacagcga tggcacttcg tgttgttttc gccgcagaac
    10801 acgttgcgga cttcgccgac gttctggacg aaatccgtgg ccgcgggtat gaagtccaac
    10861 tgatccaccc cttcgattac caaccaacag catcccggtc gagcgaccag gatgctgttg
    10921 ccattttcat tccgctggat cggggcgggg tgtttcgact cggaggatat cagtcgactg
    10981 atgtcactct tccgcgcagc gctgccacct ttctgatcgg catggccccg gaaaccacct
    11041 ctgatggcac acaaagtcat cagagacggg cgcttgatgg ggtgatcccc tatcccccct
    11101 cgttggagga tctgctccgc gtgctttccg gatgcgaagc ccggctggtt gcgggtacac
    11161 ccgactccgg ctcaccaccc gacaatgaaa cggatcacag ccgactgaag gaattctacg
    11221 agcgcatcgt ggacacaatc agcgatggta tccgggttgt cgacggaagg gatcggacga
    11281 tcgtcctggc aaacagagca cacctcgcgt ggatagggaa gcaggcgcac gaggtgatcg
    11341 gtgtccgtgc gcgggacgtt ccgtggggtt tcgacgcaaa cgattgccct gtccagatga
    11401 cggatatcgt tgatgctgcg atcgaaaccg ctgagccggc gaggcgattg atcgcaacgt
    11461 gccctcacgc gggacgtgag ctccgctgga ttgatgtagt cgcctacccg atgtcgggcc
    11521 tttcgggttc cgtcgatcac gtcgttttgg taggtaggga cgtctcagaa cgtgtgagaa
    11581 tggaaaccag ccttgcggcc gaaagagaca ggcgcgacca cgtcctccgc tcggcccctg
    11641 tcggcatcct gaccaccgat tcaacgggta cgatcgaatt cgcaaatcca ccgattgccc
    11701 aaatcatggg gttggagggc actgaagcag ttgtagggaa aaacatcctt gtgttaccgc
    11761 aactgcaggc aatggaagtt gcggccggcc tctgggaggt tcttacggag ggcaagccgc
    11821 tccatctcga ccgtgtttcc tgtcgcacaa caggcaagag agaagcaatt tgcaatgttc
    11881 gcgccgaacc gatcatatcg catgcggggg atatcagcgg catcgttgcg gtgttcgagg
    11941 acgtaacaga gacggtgctt ctacagcgga gactatcggc aacgacggcc gatctcgcga
    12001 tgctctcgga aatcggtgag ttgtttcagg attcacggga tacggaggtg atccttcaga
    12061 cgattctcgt gggagtcacg gccggcgagg gcctcggatt caaccgtgcg ttccttcttg
    12121 tggctgacca cgagacgggc acgctgcgtg gaagctatgc catcggcccg tccgaccttg
    12181 ccgaggcttc caggatctgg caggaactgg ggaagaaacc gcattctttt cgggacattc
    12241 tggagggtta ccgagaagcc atctcccgcg acgaagtgac cgtcaacaaa gtggcaaaaa
    12301 gccttgtcat ctccctggag gacgcacaga acatcgcctc caaggcagcc aaggagcggc
    12361 gagctttcct tgttacggac ggtatgaacc acccgctggt tccccgccag ctcgccgagc
    12421 aactgggcac cgattctttc gttgtcgccc cgctgatatt tgacgacagg gtcgaagggg
    12481 taatcatcgc ggacaacgcc atcacgcgca aatccattac cgaattggat ttgaggagcc
    12541 tggaattgtt cgcgcatcag gcacgtcttg cggtggagcg atcgcggttg cacgcggaac
    12601 tcgagcgcca gctcgaagaa ttacgggaag cacacgaact tctcagggcg aaccatgaag
    12661 aaatgttgcg aacggaaaaa ctggccgcca tgggacaggt agcagcgcag gtggcacatg
    12721 aaatccgcaa ccccctcgtc gccatcggag gatttgcgcg cagcattcgc aactcgcttc
    12781 gtgaagacga tcagaacagg aagtttgcgg agatcatagt agaggaaacg gcccgtctgg
    12841 agaacatcct gcgggaggtg ctggattttg cccgtccccc gcacccacgt cgtgaccgcg
    12901 ttgatgtcgg ctcggttctc cggcgtgcca cagagatgct ggaacgggaa cttcagaaac
    12961 gtagtatcag aattaacgtg cagattccgc gcgacccggt tatggtcctg ggtgacgcgg
    13021 gccagttgcg gcaggtgttt cacaatctta ttcagaacgc catggacgcg atggaacgcg
    13081 tcgacacgtc ctctcgattg ctgtccatat cactttcctg tgaaaccgat cgtcaggttg
    13141 ccgtgacggt cgaggataca ggaggcgggt ttgaagcgga cgcgctcgag catgtcttcg
    13201 agcctttctt caccacaaag tcgcatggta cggggttggg tcttgcgatc tgccagcaga
    13261 tagtccggga ccacggaggt actctttcta ttaccaacaa accggagcgt ggcgctacgt
    13321 ttacggttcg ggtgccactg ctggaaaagg aggttgtgaa tggcgaaaat cctggttgtt
    13381 gacgacgaaa acgcccttcg cgtgctgtac aaacatgaac tgacacagga cggacatgac
    13441 gtcgacctgg ctagcactgc tgacgaagca gtcgcgaagg ttcgagagac agcgtacgac
    13501 gtcgtaattc tcgatatccg gatgccggga aaggacggtc tggaggcact tactgagatg
    13561 ttcgccgagc ggcggaacat caaggtgatc atcaactcgg cgtattccag ctacaaagag
    13621 aacttcatca gctgggctgc ggagcactac gtcgtgaagt cggccgacac cactgagcta
    13681 aaagaaaaaa tcgcgcaggt gctgggagga tgaataccac caaaactcgg ttcacaccgg
    13741 gcgctctcct gcggaacaca ctgaccgtaa tccttgcagg cgggcatgga acccgtttgt
    13801 atccgctcac gaagcatcgc gcaaaacccg cagttccctt tgcgggggcc tatcgcatta
    13861 tcgacttcac cctttccaac tgcgtgaatt cgggtttgcg cagagttttc gttttgaccc
    13921 agtataagtc cttcagtctg gatcggcacc tccaacaggg atggtccagc ctccggtatg
    13981 atatgggtga attcatcctg caattgccac cgcaggaacg catgaagcgt tcgtggtatg
    14041 aaggtaccgc agacgcgatc tttcagaact tgtatgtatt tcaggagcaa cggcccgagt
    14101 acacggtaat tctctcgggc gaccacgtgt atctcatgga ttatgctccg atgctggcgg
    14161 cgcacgagaa tacgggggcg gatctcacga ttgccgccat cccggtgccc cgtcaggacg
    14221 cgaggcgttt cggtgtactc caggtggatg cgtccaaccg aatcgtggga tttgaggaga
    14281 aaccgcgaga cccggcatcg tggagagacg atcagaccag ccttgtatcc atgggcgtct
    14341 acgttttttc caccgacgtt ctggtccgga gagtactgga agatgcgaaa aacccccaaa
    14401 gtgagcatga tttcggccag aacatcatcc cgggcatggt caacagagac gcggtctttg
    14461 cctactcgat gcccgcgatg agcggatgcg agccctactg gagggacatc ggcacgatcg
    14521 acagttactg gcagacttca atggacatcc tgcggccgga ccctcctctc aacctgtatg
    14581 acccctcgtg gccggtgcga acgtaccgcg atcatcgacc acctccgatt gtcatagacg
    14641 atggccaccc tgcggggcct ttcatcagga acagcatggt gtgcgcgggg gcccgtatca
    14701 cgtccgcgcg tatcgatcgt tcgatcgtgg cactcgacaa tgtagtctgc cccggagcgg
    14761 atatcgagga aacggttctg atgagccggg tgcgcgtggg caaaggtgcg cgaatccgaa
    14821 gggccatcgt tgacaaagat gtggacatcc cggcggggga gaccatcgga tacgatcttg
    14881 aacgagagcg gaaacgtttc ctcgttaccg acaacggcat cgtcgtcatc ccgcgtggct
    14941 acgtctttga ctgattccct gtttgcacga ataccggagg cagtttgatg caggaactcc
    15001 gcaaagaccc ggtgacagac cgctgggtca tagtttccac cgagagggga gcgcgaccaa
    15061 cggatttcga atcgttgccg cggcccaagg ctccgcagct ttgtccgttc tgttacggat
    15121 tcgaatccac gacgccgccg gagatttact gcgtgaggga agcgggtacc gcacccaaca
    15181 cgcccggctg gtccgtgcgg gtggtgccca acaagttccc tgctctgcga atcgaaggaa
    15241 accttgtccg ggaaggggtc ggcatgttcg acaaaatgac cggaatcggt gcgcacgagg
    15301 ttatcatcga gcatcctgat caccagcggc gtttctggga cttttcggtt caggatattg
    15361 agcgcgtcct tgccgcatat cttcagcgca caaatgacct gaagcgcgac attcgattgc
    15421 ggcatatgtt ggtgttcaag aacgaaggcg aacaggcggg tgcttcagtc gctcattcgc
    15481 attctcagct cattgccacg cccgtcgttc ccaagcgcgt gcgtgagaaa ctggacggcg
    15541 tcaaccggta cctgagttac aaagaacggt gcgtgtactg cgatgagatt cgtgaggaac
    15601 agcggtttgg agagcgcgtt gtttttgaaa atgatagctt catttcgttc tgccctttcg
    15661 cctcccgctt tccgttcgaa gtgacgctcc tgccgaaaac gcatcgcccg gatttttccg
    15721 ccatcgacgg cacagaacga acggcactgg cagaggcgct gcgggtcacg atgcggaaac
    15781 tcaatgaagc gctgaacggg ccccagtata actggatctt tcttgcggcg ccaacgagat
    15841 tatcgggacg cgaatcgtgg gaggatttcc actggcacgt tgagataatc ccgcgcctta
    15901 ccaaaatcgc cgggttcgag tggggaaccg gcttctacat caatccaacc ccgcccgaac
    15961 acgcggctgc gtaccttcgt ggcgtggccg ggtgcggatc gccagggttg gattgatgtt
    16021 cacttcccct ccttccggag cctttcccac agatgcccgt acttaacgtc gcactcctct
    16081 ggcatatgca ccagccagat taccgtgatc ccgagactgg catgctggcg ctgccgtggg
    16141 tgcgccttca cgccaccaaa gggtattacg acatggtgaa ggtgatcgaa cgggcagata
    16201 cacatactca cgcctgtttc aatttcacgc cgagcctcgt ttcgcagctt caggggtacg
    16261 ttgacggaac gcttacggac aagtacctcg aactttcccg gcggcctgca tcggatcttt
    16321 cggcagagga aagacgcttc gtcgcgagca actttttcca cgcgaaccga agaacgatga
    16381 tcgactgtta cccgcggtat tccgaactga gaaccatgtc gcgtctccct tccgggagcg
    16441 ttcgagattt ctcggatcag gacctcactg atctcgtcgt gtggttcaat ctcacctggt
    16501 gtggctacgg cgcgcgcgcc gatttcccct tcctgatgga attggtccga cagggcagag
    16561 ggttttccga ggagcagaag ctccagatgc tcgctcttca gcgggacatc atcgcggaga
    16621 ttctcccgtc ctaccgcaag gccgcggaag gggaacggat cgaattgact tcgactccgt
    16681 tttaccatcc gatcctgccg ttagtgtgca caacggattc ggcgaaagcg gggcttccgg
    16741 acagggaact cccggccccg ccgttcgtcg cgccggagga cgcaagacac cacgtaagac
    16801 gcgccatagc actgcacgaa tccgtttttg gaagccgccc cgacggtatg tggcctgcag
    16861 aaggatccgt gagtaccgaa gcagtacaga ttctcgcgga agagggtgtc aaatggatcg
    16921 catcggacca ggacgtgctg ttccgttcga atccgtcgct gggacaacga gacctgtatg
    16981 tgccgtattc cgtcggcgaa cgaggcggcc aactctcgat tgtgttccgg gacaagtctc
    17041 tcgcagacaa aatagggttc cgctattccg gaatgacgac tgatgcggca gtgaacgact
    17101 tcatggacaa tctgcgagcg atctggaccg ggcaacccaa atccggtggc gaacagctcg
    17161 ttactatcgt gctggacggc gaaaacgcgt gggagtacta cccggacggt ggggaagcat
    17221 tcctgacggc catgtactca aaggttgcaa attcgaagga tttccgatgg actaccatct
    17281 ccgactacct tgccagaaac cctccgcgac atgccatccg aaggattttc ccagggtcgt
    17341 ggatcaatgg gaattttgac atctggatag gatcgagtga ggaaaatcgt gcgtgggaag
    17401 cactgcgtga cacacgcagt gctctgattt cggcacagga tcggttatct gagaaggctc
    17461 ggcaggaggc ctgggagcac atctacatcg cggaagggtc cgactggttc tggtggtacg
    17521 gggatgattt caccaccgcc ttgcagggcg agtttgaccg gcttttccgc gcgcatctgg
    17581 cggctgtgtt cgaactgatc aacgtaccgg tgcccgcgtg gttggtgaaa ccgatcagga
    17641 aaggccggga actggccgcc agcaagccgg caaatctcat ttctccgacc ctcgacggcc
    17701 ggtcaacgag ttactacgaa tgggctggcg ccggccattt cgatgctcgt tctgcagacg
    17761 gcaccatggc aagggaagcg ccgctggtct cagcgatcgc gttcggcgcc gatcatcaca
    17821 ggtggtacct cagaattgac tggtccagac cgttggcgcc gaacgagcgt tcggaattgg
    17881 aggtcgtttg cgtctttcca aacagacctg gtacgcaggt catcatcggc ccgtttgggt
    17941 tcgagacgcg ggacatcccc gtcaagattg tggaacacgg gactgaagtc cagatcactc
    18001 cgcaggccgt ttttcgcgat ttggttgagt gcgccgtgcc tttccttcta ctcggcgccg
    18061 ccccggggac gctcgtggaa tttgtggtga gtgtccgcca agggggcgat gaaatcgagc
    18121 gttggccgcg tgacggggta ctggcgttcg atgtgcccac cgacacattt gagctggagc
    18181 actggaccgt gtaacatggt gctggatccc agaaccctcc gggtgaaatc gatcgcctac
    18241 atacgagagg cgctggccaa ttgcggggca gtctctgatt ctgtcatccg tgcgatggag
    18301 gaggatgacc gggcagccgt ccgcaggatg gcggagcaac tgaagaaggc gcctagccgc
    18361 ccgcataccg acatgttagt ccacgaacaa caatgctggc aaagcggttc cgtgcgggtc
    18421 gccggcgtgg acgaagcagg cagggggcct ctcgcgggtc ctgtgtacgc cgccgcggta
    18481 atcttaccgc gcgatggtat cccatctgga atcaacgatt cgaaacggct gtccgcccga
    18541 caacgggagc aactggatcc gcttatccga gcgtgtgcgc tggcagtgca cgttgcatcg
    18601 gttggtgtcg aggaaattga ccgtgtgaac atacttcagg ccagcctgaa agcgatgcgc
    18661 acggcgatcg caggccttgg acaccctcct cccgatcatg tgctggtgga tggcaacatc
    18721 acccccggaa gcgggctccc ggagactgca atcgtcgatg gcgactccaa atccgtttcc
    18781 attgcggccg cgtcgatcct ggcgaaggtc gcgcgtgatc gcttcatgga ggaaatggac
    18841 acgcggtatc cgggttacgg gtttgcgcgg aacaaagggt acgggacgcg ggaacatctc
    18901 gaggcgcttg cacgtctcgg cccgtgtccg attcacaggc ggtctttcca gcatgttgcc
    18961 tgcgcgtctg aatatcccac ccattcggat cttatagcgt tcttcaagcg ttcgcttgtc
    19021 gtgtcagagt ccctcgaaga gctccgttct gtcgccgcaa tcatagccga aaaaaagaac
    19081 gtgctgccgc ccgaagaggt accggcgttg aggcggttgt accggcaaca attcaagcta
    19141 cttcgcagca ggtgcgatac atgaaacgcg gcagattggg ggaggaactg gcggcggggt
    19201 acctgaagcg ggccggttgg acgctctgcg cccggaactg gcacgcaggc agagccggtg
    19261 agatagacat tgtcgggtgg gacggcgata ttcttgtttt cgtggaggtc aaaacggcga
    19321 atacaagtcg ctacggacat ccggtcacgt gggtggatgc tcgcaaacaa tctcaacttg
    19381 cgaggctggc tgagttgttt gtcgcaaagg gagatttcgt tttttcggcg atgcgtttcg
    19441 acgtcataac cattgacgca gagaagcagc cgccggcgat cacccatctt gtggacgcct
    19501 ttcggcctga catcgtggga gagacagggt gatggtgtgc gcccggcgag agagaaagag
    19561 aaaactggtt atctgacagc ctgaattctg gcgaccaacg aatcgcccca gacttcgcgg
    19621 taccgcctcc acaccttttc cgctcgatct ctgaacaggc ggaggtccgg gcggatcaca
    19681 atcatgccag attgctcgaa ttcgtgaatc aattcccgtt cttcttcctc cacgatctgc
    19741 cggttgtatc tcccaccttc atggaaggct tccgtaatga ggagccgatc acgttccgac
    19801 agagttcgcc agaacgcggc gctgacgata ggcatgatcg gcgtgatgct gtgattggtg
    19861 agaacaagat atgagcacac gtcgtggaaa tgcatcgctt caatcgttgg caacgggttc
    19921 tcctgaccgt ccaccgtgcc cgctcgaatc gccgtgcgaa catcattgat gttcatcgtc
    19981 gtgggaagcg ctcctagcac gcgtgcgcat tcgacgtaca tctgagaagg cggtacgcgc
    20041 agtttgatcg tcccgaactc cgcaggggaa cgggccgcct gatcccgcag agtgacctgg
    20101 cgtacgccct ggtgccacat gtcaatcacg tggagtccag atcgccgttc aacctcggtg
    20161 acgagggagc gccaaaaagg tgtctcgaca gtgtgataaa aatggtcgat atcgcgaaac
    20221 atgtacggcg cttcaagggc gcccaggggc gggtagtaat cggagaggct tcccatccct
    20281 gacatcgaga tttcgtcgct ccctttgagc atctcatcta tctggtacgg aagcgaaagt
    20341 cccgcttcca ccaccaggtg aatccgaccg cgagaccgtt cctccagtat ggacgcaact
    20401 ttgaggagtg cgcggtgcat cggcatggcg gggggtgcac cgtggcgaac ccgtaacagg
    20461 acctcggttt ctctcggtgc gcacgctgac aacagcccgc ccgaaaggac cgcggtcagc
    20521 aacgccacac ggagttttgt ggaaagagaa gtcaccatcg gcgccgcgca ttccccgcat
    20581 caaaaacccg aaagggcctc ggattcgctc gcgtagattt ccacgtactg tttgagaccc
    20641 accatcgcaa atatcttctg aaaatgctcg gaaggctcga ctatccggat cttctgcccg
    20701 tgtttccgtc cggcgatggc gacgttgata atgatggcga tgcccccgct gtttatgtat
    20761 gcgtctttgg tgaacgtcag caagagcttc tttgccccgg attcagtcgc gtgctggtag
    20821 gcagcctcca cttcctcgcg ggtctgcgcg gtcaggtccc cgaggatatc caggatggca
    20881 acgtcgccag caacacgggt tacaacccca tttctcttta cttcgtcagt tttcacgttc
    20941 cggtcccctt aattcgcgag ttctgaagca actgctgccg gttcagggcg tatacgcgtt
    21001 acttcccggg aggttccagc cggatgatca tgcgcgtgac attcccgagt tcggaacgaa
    21061 cattgaactc cacttcgtcc atcaacgctt tgatgagaaa catgccccat ccgcgggctg
    21121 cctgttctcc ggagaatttc ctggaaatat cgggatcggt ggggttattc tgcatgccgc
    21181 tgccctggtc cacaacgtca atccgtaacg ttgaatcctg aacggtcagt gccacgacta
    21241 ctttcatagc ctcattgaac ttgtgggcgt gttccatggc gttgatacag gcttctgcaa
    21301 ccgccgtttt gagatcctca atccgcgatt gccggaatcc tacgagtcgt gccacggacg
    21361 ccgccgcatc catcgcgacc cgttcgtacc ccgctgtact cggaacctga agttcgatag
    21421 tcatggcatt cgatcccata cctgaccccc tgtcccgtag gtaaccaccc taagtccacg
    21481 gcgcgtagcg cggtatcaac cggtaacgcg aaccgcgatt atcgtgatgt catcgtcttg
    21541 ttcatgttca cccatgtgcg cggttacttc cgtgacgatc gagtcacgga cctcgaacgc
    21601 agtactacct ctcctgacag cctcctcggc tcgcccaaaa ccaaacatct ccccggcagg
    21661 agaccgcgct tccaccacac cgtccgagac gagaacaacg atgtcaccaa tcccgagctt
    21721 cttttcgcat tgctcgaacc tggtgttttc agcgacgcct aacgggagac gttcgccttc
    21781 gacggatatc gcggttacgc gatcaccgga acgatgcaga ggatccaatc ctcctgcatt
    21841 cgctatctgc aaaatacctc tttctccatc gaacagagcg accgtgcaac agatataggt
    21901 atttcgggac gcccggcccc agaaggtcgc gttcagcaac tcgagcagtt cagcaggttg
    21961 cgtcgtctgc cgcgcagccg cagtcaccat tccgctgaac agaaccgccg ggattgccgc
    22021 ttgcatggca tgtcccgtca cgtcggcgac aataacacca aacttcgatc gatcctcgcc
    22081 gtcccagaca tacgcgaagt agtctccgcc aacccggctt gccgcgcggc agatgcccgt
    22141 gatctcgaag cccggaacgg cagggtctac gctcggaaga agagcattct ggagcttctg
    22201 ggcaacctgc aactcccgct gcaattcact cagaagctga gcctgcgcac gcttccagcg
    22261 tcgattccgc tggtaggcga tggccgtggc gaaaccgatt cccaccgcgg caaccacagt
    22321 cggaacgacg aaccagggtg ttctccagaa gggtggttcg acggcaaacg tcgcggaaac
    22381 gcccgtccca tcatcgttcc cctcctgatc cagggttctg accgtgaaaa catgctcgcc
    22441 ggctggaagc tcctcgaacg tgtgcgcggt cgcgggagaa aagggggacg gttcaccagc
    22501 atccagtcgc catgaataca ggaggtcctt tgccggcgtt ctcccaaacc gatcgcttcc
    22561 agtccagcga acggtcaaga accctgggtg cgcaacccgt tctggcaacg gttcgatgaa
    22621 agaccttggc ggatccgacg acgtggcatg gtatctggac gccgccccgt cgaaatgtcc
    22681 gaacagtaac gagccatccc ccagcagcgt tgatgcggac acgtggtcct cagcaagtcc
    22741 gctttccttt cggaacgtga gccactggcc acggaaatac cggctgacac cacccgctgt
    22801 ccctacccat attgttcggt attcatcctc tacaatggac cagacgtcgt cgctcgccag
    22861 tccgctatcc accgtgaaat gcgtccatct tcctcccgcg tacctgctga ctccggcgtt
    22921 cgggctgttg cctcccaccc agacggatcc gtcgcgagcg gcgcacagtg ccgatatatg
    22981 ctcgaatcgg acgctgttct gcccatcgaa agcgctccat cgtacaccgt caaagcgact
    23041 cagaccgtta cgcatccctg cccagatgcc gccggccgta tcctctgcga gcgcgagcac
    23101 gtcatcggtt cccgcgccct gcgaacccga cagtcgttcc cacgatccat ttctgaagcg
    23161 gaatacaccc cgcgccttga catcgccaga tactcccgcc catacatccc cgtttctgga
    23221 gcgcaagaac gcatgaatcg ttgatttgcc acctgagggg agaaacgggg gaatccttcg
    23281 ccattgactg gcgatgtggc gagggctccg ctcccagatc gtcggcggag gaaccggtga
    23341 cgcaattgca acccataatc ttccatccgg gaaactcagc aacgccgaaa cgggcttccc
    23401 acgcaattcg gcaggaaccc ggtcatccga aaaccgcttg ccatcaaaca aaagcagccc
    23461 gtgtgtggtc ccgacccact ggattccatc gccggtacgg gcgaaacaac tcacacgtgc
    23521 ggactctggc agcccatcgc cggcgtcata tgaccaccat ggaccgccgc ggcggtcaaa
    23581 ccgcgcaact ccccggcccg ggatcacaaa ccagaacgcg tcgtccagcg catacacaat
    23641 cttgctcacg agcctgccgg taacacccac ctgccggacc gaatgtccga tccacgcgcc
    23701 gtcgcgaaac tccagcagcg tgcctgcggg agacccgaca gcccagagcg acccgtccgg
    23761 catgggcaga agagacgccg ctgtaaacga tgacgcaaac ctgtctccga caaacctctc
    23821 cagagacccg gcccgccacc gaagaacgcc atgaccggcg gtcccgaacc agatcgcgcc
    23881 atcacgcgtc tgggcgaggc tgaggtactc cacggacggc aatccgattt cgcgcgccac
    23941 gttcctccac gtggcgccag agtacacata gatctctgag tgcccgttgt tgtccccgaa
    24001 aatcgcccac accgagccat cctgatcacc gaaaagaaca cgcaccggca gggaagggag
    24061 cgttccgtcc gcagatgtga attcgcgcca agacctgcgt tcgtacacgc ttacggtggt
    24121 tctcggtcgc ggcgctgccc cccgcttgtc ccgcgcgaac tgtccatctt cggcagggga
    24181 agcacccacc catatcgctc cgtcagcaga ctccgccaag gcggtgatgt tgccctgctg
    24241 aacagggtaa tgccactggt gggctatgta tttggcaagt ccctcactcg tgcctatcca
    24301 gagagtcccg tcggcggaat agaggaggtt gtggaagcgt gcggacgggc cgattcctgg
    24361 gatttccttc ttcacgaact gagtgccgtc gtgaagcgca agaccgcggt ctgtagtaag
    24421 ccacacgccc ccgccaatct cctggacaag gtcgttggta tacaggctgg cgatctcttt
    24481 cgggtcttca aagaaacgcc agcgtgagct ttccagcagg gggtctcgtg cggtggcgct
    24541 tgcagcggcg gacaaaacga tcaggatcag acgtgcgcaa accatcaaag gggacgctcc
    24601 aatctttgat cggcggtgca ctctcagcaa cccgcaccgc aggtcgtcgc cggatccgga
    24661 agtcgtcatg cgtgtgaaag aaacaatacg cctgttccgc cgtgttccgg ttgcatcaac
    24721 cgagactgga actggctgtt gcgcgaaatg ccttgcgaat tcctcgagcg ccaccttcgt
    24781 ccgtctcggt gggaaaccat tcgtccatcc atcccgccgg cagtggttcg cgcagcatct
    24841 ggtcacgtgt ctgccagaga agcgcaaatc cgttttccgc cagaaacccg ctggcaaggg
    24901 aaagcgattc cctcgtgtgc gccacggctc gaatagtcac aatatcaacg caaccggcat
    24961 agcgcgaatt ctgggcgagt tccgtagagt ccgcccacgc cacctccacg ttgtgaagct
    25021 cgagctccaa cgtcgcccgt ctgatgaacg cggctttttt tcggatccgt tccacgcagg
    25081 taattctggc actcggaacg gcaatggcaa cgggcagacc cggaaaaccc gccccgctcc
    25141 ctatatcaac gacgttcctt ccacaaagat caacgaagtc cagtacttgc agagagtcgc
    25201 agacatgccg ttcccagagt ttcggaatgt cccccggcgc cacgaggttc aaccgggtgt
    25261 tgctttcgtg cacaaggcgg accagcccaa gaagctgctt gcgctggaca tccgtgagat
    25321 gacgctcgaa tgatttcacg ggacctctgt gcgtcgtctt cgcgcttcca acgcgattgc
    25381 gataacggta atgtcggccg gcgttatgcc gggtaccctt gccgcctgac cgagagtcgc
    25441 cggacggacg gttcgcagct tttgtcgggc ttccgcggac agagctttca ccgcgtcata
    25501 cgaaaagtca ggtggaatct cgcgattttc cgccgcggcc tgacgttcgg cggcctctcg
    25561 ttgccgaacc aggtagccct cgtatttgac ctcaatctct gcctgccagg caatttcccc
    25621 gtggatcccc gccatatcct cgctgaccat tcgcacaatt tcgtcgagcg tgatctccgg
    25681 ccgtttgagc agtcgatatg cggattgcgc ttccgtaatc ggagtggtgc ctttcccggt
    25741 aagcaccgga ttggctttct caggcgggac aggaatctct cgcagccttg aaacgagcga
    25801 ttcaaccgcc cgcgccttct cttgccgccg tcgtaactcg tcccctctga tcaggcctat
    25861 ttcccgggct cggtcggcca gccgtacgtc agcattatcc tgccgaagga gcagacgatg
    25921 ctccgcccgg gaagtgaaca ggcgataggg gtcctccgtc cctttggtta ccagatcatc
    25981 aatcatcacg ccaatgtacg cttcgtgacg gcgcagaacg atcggcggat cggcggaaac
    26041 tgaacgcgct gcgttcaccc cggcgatgaa cccttgcccg gccgcctcct catatcccga
    26101 agtcccgttg atctggcccg cgaggaagag ccctttcacc gccttcgctt cgagacttgg
    26161 tgtgagctgg tggggaaaca cgaagtcgta ttcgaccgcg tagccgaaac ggttcacaac
    26221 ggcttcttcc attccgcgga tcgttcgcag ggctgcaagt tgtacatctt cggggagact
    26281 gctgggaaaa ccgttgacat acacttccga cgttgccgtg ccctctggct caatgaatat
    26341 ctggtggtgg tctttttcgg gaaaacgcac cactttgtcc tcaatggaag ggcagtagcg
    26401 cggaccggtt ccttttatcc tgccggtgta gagaggagaa cggtccagtc ccccgaggat
    26461 aagtctgtgc gttgcggcgt ttgtccaggt gagccagcag ggaacctgtt gcactgatcg
    26521 cggcggcgtt tcaaacgaga agaaaggcgg cggttcgtca ccgtactgag gagtcatcac
    26581 cgagtagttg attgtgcggc cgtcaacgcg aggcggtgtg cctgttttca gtctcccgag
    26641 tgccaaccct cccgctgcaa ggcttcgcgg aagctctgag gcagccggtt cccccacacg
    26701 gccgccaacg gaagacgaca acccgacgtg catcaacccg ttgagaaatg tgccagtcgc
    26761 aacaaccgct gccctgcacg aaatctcggt tccagagacg ccgatcactc cgacgatgcg
    26821 gcctccctca attctcagtt ggcctgcctg ctcggccacg attctgacgc gttcgtgtga
    26881 ggacacaagg gcctgtgccg cggccctgta cgcggcccgg tcagcctggg cgcgcggaga
    26941 ccacattgcc ggtcctttgg aacggttaag catcttgaac tgaatacccg tggcgtcaat
    27001 caacttgccc atcacaccgc caagcgcgtc tatctcgcgg acaacatgtc ctttcgccgt
    27061 gccaccgatc gccggattac acgacatttc cccgattttt ccgacatcca ttgtcatcag
    27121 gaccacgtcg aacccaagcc tggcagcggc catcgcagct tctatgcccg cgtgaccgcc
    27181 accaataacc acgatatcgc aggaaagcaa ttatgtgcct ttcgttcgcc caacaaaccg
    27241 ctgcagactc accacttgcc gccatggaaa cccgtggcag tcaacagagt aaaaatagca
    27301 cggaacgacc agtgtcgcta aatgcgatca ggacgcgaca tttggctgcg tcccctcgcg
    27361 cgggtacatt caaacgatgt agccgagcac cagaactaca acggcatgcc ggacataccc
    27421 caaaatgaaa atgcgcaaac ctcgcctcgc ggtgaccatg ggcgatcccg ctggtgtagg
    27481 accggagctc tgcctcacgg tggcaactga taccagggcg acaggtgcgg ccgacctgtt
    27541 gtttgtggga accgagtcca tactgcagag cgtcgcgaaa accctcgggc gcccgcttcc
    27601 tgaccctgca cacatctgca gcgtcgcgga acccaaggtc gctgtcccgc cgggtgccgt
    27661 atcggcagag gcgggcagac tctctgccct gtatctggat cgtgcgatag tccttgccca
    27721 gaagggcgaa gtggacggga tagtaacggc accgatatcc aaggaagcgt ggcatctcgc
    27781 aggagtccct tttcccggcc atacggagtt cctggcggcg aaaacgggaa cgaaggaata
    27841 cggcatgatg ctcgtgcacg gtacgtggcg ggttctgcac gtcagcacgc attgctcgct
    27901 ccgcgatgca ataagccgcg taacacagga gcgcatcttg cggatgcttc ttctgttcga
    27961 cggtgtgttg cgtgatctgg gaattgcgtc gcctcggatc ggtgtcgccg ggctcaaccc
    28021 ccatgcgggt gaagcaggtc tgttcggcga tgaggaacgg gagcatatcc tgccggccgt
    28081 tctcgaggcg cgctcgagag gcgtcgaggt actcgggcca atccctccag atacggtatt
    28141 cgctcgcggc cggctggggg aatacgacgg tgtgctcgcg atgtaccacg atcaggggca
    28201 tatagcggtc aaaaccctgg ccttcgagcc ttcaaaagac gggggatggg catcagttca
    28261 cggagtcaat gtaacggtcg ggcttccgat cgttcgaacc tccgtcgacc atggagtagc
    28321 cttcgatatc gccggaaagg gaatcgcgag gaccgaaagt atgctggatg caattcagct
    28381 ggcggcccga atggccttgg tacgcgcagg aggcagcgtc cgatgactcc agatgacagg
    28441 ccggccgtca ccgggagcac ggaagcgaac accggggcca aaggcattca acggtttctt
    28501 accctcccca ccgctgccgc gacaggtgcc tttctcgcgt tgttactcat tttcgttctg
    28561 ggcaactggg tagtaatgcc gcttatcacc cgcggaacgg acgttgtcgt tccagacgtc
    28621 cgtggaatgg acgtggccga cgcaaaacgt ctggttgagg agcaaggtct ggtattcgtg
    28681 aatgactcgt cggactatgc cacggatgaa tccatccctg tcgatcacat agtcatgcaa
    28741 agccccgctc ccgacgtttc tgtgaagcgc ggacggagcg ttcgagtaac cgtcagcaga
    28801 ggcgctccaa agtacgttgt acccaacgtg cggggtattt cactcaacga cgcccgcgtc
    28861 aggcttgaac aggcgcggtt cagagtcggc aacgtcagtt acgtgctccg gacaaacgag
    28921 gacctgtctg aaccctacgt tcaggaccag ttccctgatg cgggaacctc gctgcaaaag
    28981 gacgctcagg taaggctcgt ggtgagcatc gctcccgaga tgcctgctct ggtgggaaga
    29041 gacctcggtg aagcaagtcg aacccttgcc gctatgggaa tccagattgg agaaacgacc
    29101 tccgaacccg atgagaccgc tgcgccgggg acagttctga agcagtccat tgctcccgga
    29161 acccgggtgt tcgccagtga ccgggtcgat ctcgtggtaa ccgaataacg cattcaggcg
    29221 gcgtgccgat gagaatcatc ctgagggatc tcagttaccg gtacaacggg gaagccggag
    29281 tcacgaacct gaatctccag gtggaatccg gggagttgtt cggtcttctg gggccttccg
    29341 ggtgcggaaa aacaacgact ctgcgtcttc ttgcggggtt catccaacct gagcacggcc
    29401 acgtgttttt tggagacgaa gacatcactg gcaagcgccc tgaacaacgc aacgtcggga
    29461 tggtattcca gagttatgcg ctcttcccgc acatgactgt gcacgagaac gtcgagttcg
    29521 gccttctggc tcgcggagtt ccgagaaagg aacgcggtcg ccgcattcgg aacgcgttgg
    29581 agcttgtgca gctggcaccg atggaacggc gttccgtagc tgacctctcc gggggacagc
    29641 aacaacgcgt cgcggtcgct cgagcgctgg tgatcgagcc caccgtgctg ctgctggacg
    29701 aaccgctttc caatcttgac gcgcggctcc gtgaagaaac cggccaggag cttcgggcac
    29761 ttcagcgacg actcgggatc acgacggttt acgttaccca cgaccgtgcg gaagcgttcg
    29821 cgctgtgcga tcgaatcggc atcatgcaac acggcaggct gattcaggtc ggtgcgccgc
    29881 aggatctttt tgccagtccg ggctctcttg ccgtcgcgca gttcatcggg cacaccaaca
    29941 tcctacccgt agaacatatt caagaggcgg gaaacggctg gacggctcag acaggtcccc
    30001 acaccttatt tggaacgaga actcttccgc aggaaccgac ggccgcgctg gctgttcgcc
    30061 cccatgaaat gacactggat accactccca tctcctcaaa aaacaccctg ttgatgagag
    30121 tctcgggccg acgattcctt ggcgcggaca tggaaataag cgttcaggac gggaacctga
    30181 ggctgaagat tctccagcgc ttctcccaat cccgcgtgcc ggaggaggat tcgcaggtat
    30241 acgttcatat cccgccggaa gcgctgatac ctctggcacg atgagccgct gaagcgacaa
    30301 agcgggagaa caggatatgc cgtcttcggg tttgatcgag aagacatacc gacagaccct
    30361 taaccaggcg cgcgagatcg tgtcagagcg gctatccgct ttggtccgcg cgacaggagg
    30421 cgactgcgag gatgccgccg aggacattgc gcaatccatc gtcggcacgg agcaggtggt
    30481 tcgcctgatt ctttccaccc cgtacgatcg aagcctgtat cgtcccgttc tgctggaagc
    30541 agcggacgag gtcgggagga catttttccc ggagatcagc aagcgtttct tctctcctct
    30601 ttctgatgct cctgccgttg aaagcaacgc tgcggaaccc gagcgatccc tggactctcc
    30661 tcaactggag caggcggccc ttgctctccc cccgcacctg cggtcgtact tcaaaaagct
    30721 cctggagaac gcggccgaaa ggcttcccac tgcccgcctg tcggcgatcg caaccgatct
    30781 gctcgcggag aggctggaac gcctccaacg tcactttcct gacggcagta tcccgccctt
    30841 gagcaacgct cccgtaacgg accggatgtc tgatgacgag ataatcctcc acgcacgata
    30901 tgtagccgca cgtcttgccg cggcctttcc gtggggtttc ttctcaaaag acacccgccg
    30961 cacaggaatc gcggtgaact acctggtgat gcgcgaaatg cgaaagaccc ccgaggaggc
    31021 actgcaggag gatgtgcttt cgtacatcgc cgttgggttg ggaccagctt tgcggcgctg
    31081 cgggggctcg gtgaacaggc ttcttgcgct tgggtttccg gaacgaatca agccatggat
    31141 gggcagccac gttcccacag gctactggga agaaaccgca aatcgccggg acgccgtgaa
    31201 atggcttgtt gaacagcgcc tcggcattcc acccgaagct atcggacggg ctgtgcacca
    31261 gaacatcatt tccaaaaagg catttcgcga agctggattg acgtggctgc tgaaaaacgt
    31321 ctatggctgg tcggtcgcac acgcgcttgc cgagtcgtac ccgcagctcc gcccctggga
    31381 gtgcttctgt cgcgtgccgg tggaaacctg gcacggcgac gccgggcgcc tcctttcctc
    31441 ggaagcaatc ggctgggcat tccgcgaaga aggtatgcag gtcgcgatgt tccgcacgca
    31501 ggacgccgct cgtgttatca agtccacgct gaaccgctgg catctgggcg ccgcctttcg
    31561 aatcggattt tctgagaact cgttcgctct tctgaattcc ctgttcccga acacgttcga
    31621 actgtgggaa gtgacccatg tgccccgcga ggcgtggaag gacccggcgc tgcgcaccaa
    31681 cgcggtccaa tggctgctga aagagtatca tgtcgaagtg gagaagattc cggttgccat
    31741 caggaacggc ttactctcgc ctgacgtttt caagatgaga ggtttggatg gccttctgag
    31801 gcagatggga agtgtctggc gagtaataga cgatgtgttt ccgggaaggt tcgcgcgttg
    31861 ggagcttggt tctgtgccga attcctactg gcgcgctcgc tcgagcgtcc gggaagcggc
    31921 aatatgggcg ctctcccgcc tcgggatcga actcgcccgc gttgaagatg cgttgcgaag
    31981 cggaacgctc gcgtcacgag cactcactga aatggggttg ggctcgcttg tgaacacgct
    32041 ctttcgcggc gatgtgcgct cgctgatgca agtcgccggc gtgttgccat cagcgaggag
    32101 agtacctctg cgccggtttt accggcagct caggcaggac cttgtcgctt cgcacgcaag
    32161 cgaagttgag gaccgtgtgc aagcgcttcg cgccagacag aaagaacgaa gaaggcgaac
    32221 ggaagagcat tggtgttgag caacccatga aacggaggct tttgtgcgaa tcggtattac
    32281 gtgtcatcac acgtacggcg ggtcgggaac ggtggcaacg gagcttggaa tggcgctggc
    32341 caaacgtggc catcaggttc atttcgtaac attcgccgag ccgtaccggt tgcagtttga
    32401 tccgaacatc tacctccatc aagtggacgt tatgcactat ccgttgttcg accatccccc
    32461 gacaacgctt tcgctggccg caaaaatggc agaaacgatc tgcacggaga agctcgatct
    32521 cctgcacgtt cattatgcga ttccacacgc aaccgcggcc tatcttgctc tgaagctctg
    32581 ccctgaccac aacgtccggc tcgtcactac gcttcacgga acagacataa cgctcgtcgg
    32641 gcaggaccct tcatacttca gcattaccag gttctcgatc gaacaatctg acgtagtgac
    32701 tgccgtcagc cagtggctga aggaccagac gctttcacgg ttcaatgtgg accatccaat
    32761 cgaggtcatt tacaattccg tggacacgga gagattccgc cgagtctcgg tgcctgcatg
    32821 ccagagagcc cgttacgccc ccggaggaga acccatcctc gtgcacattt cgaacttccg
    32881 cccgctcaaa agggtgacgg acgtgatcag ggcattcgca ctggtacgca aacagatgcc
    32941 ggcgaagctc ttgatgatcg gtgacggacc acaccgcatg cccgcgcagg aagtggcgct
    33001 ggagctcggt gtgatggggg atgtctggtt cctcggaaaa caggacaggg tggagctttt
    33061 tctcagcctc gcggacctct tccttttgcc aagcgaacac gaaagcttcg gtctcgcagc
    33121 tctcgaggca atggcgtgcg aggttccggt catcgcatcc cgggctggtg gtctggtcga
    33181 ggtgatcgaa gacggagtgt cagggcatct ggttgaggtc ggtgacaccg aaatgatggc
    33241 agaactggca accagggttc tcaaagacca ggaactggct gcccgaatcc gagccgccgc
    33301 tctggaccgc gtccaccggt tattctcggc cgacaagatc gtcgcggaat acgagcagct
    33361 ctaccgaacg gccctcagct cgccaaagca ccagtcctga acgcacacgg ggcgcgcagc
    33421 aacccgccgc aaggtctggc tgaacttcgg cgccttcgat tcggggcggt tcagccccga
    33481 acctcagcgc cgtccatctg ttccaacgct ttcaccagtg cttggatgcc ttcgtcgccg
    33541 caacctttcc cctccagact ctggaaaagc tggttgacaa gagccgttcc gggcaacgcg
    33601 atctgaagct ccttcgcggt atccagcacg agacggagat ccttctgctg gagtttcacc
    33661 atgaaccccg gggagaaatc ccttttcaga atcttcggcg cgagattgtt catcgcccac
    33721 gagcccgctg ccccggcgga gacggcgtcc accattttgc tgacgtcagc ccctgatttc
    33781 gcggcaagaa tcagcccttc acacattgcc aggtcgttca gtgcacccac tatctggttg
    33841 cacagtttca ccgtctgccc catgccgttg gggccgatgt ggatgatgcg tttgccgagc
    33901 gtttcaagga tcggtgtggc ggtggcaacg gtttcggcgt cgccacccac catgatggac
    33961 agcgtcgcct cgattgcgcc tttttctcct ccggaaaccg gcgcgtcgag catttttgcg
    34021 cccttcgctt caagagcgga tgccacttcg cgcgctactg cggggctgat ggtactcatg
    34081 tcgatggcga tcatccccgg tcgtatggta tcaagaacac cgttgggccc gagatacacc
    34141 tgcctgacgt ccggcgaatc tgtgacgcag gaaagcacga tgtcgcacct ggaagtgaca
    34201 tcggccggtg aagcagcttt ctgagctccc tccttcacgc acgccgcgat accgggttca
    34261 ctcctgttcc acacagtaac ctgataccct gctttcaaga ggttccttac catcggacgt
    34321 cccattatgc ccagcccgat gaaccccaga cgtggtttcg acattgt
//
LOCUS       DAOSOO010000011        30566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1514_length_30566_cov_5.411163, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000011 DAOSOO010000000
VERSION     DAOSOO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 30566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30566
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_30566_cov_5.411163"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2307
                     /gene="priA"
                     /locus_tag="PLT86_01695"
     CDS             <1..2307
                     /gene="priA"
                     /locus_tag="PLT86_01695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT35359.1"
                     /translation="AVHENGSEPAQVVLRPVADFPDPSPSLDLTMLSLTRWIADYYLS
                     SWGEAIRAAIPHGLGTNSVWMVHLSCENPHVVADNLQARFPKRAEILRYVAEKGQVSL
                     KTLARVFSGTGFRSNLQALQNDGHVKVRDEAEPIVLSRVRTEKLVQLAPAYLDPDATR
                     RMRFGAEQLRVIDGLRTISEPLSVASVRSRFGVSTATVNRLVARGVLNVEEHEVDREP
                     DYGECEDETGQLLTNDQRDALGVILPNVASRKFSAVLLHGVTGSGKTRVYLDAIEHTL
                     DAGRGAIVLVPEISLTPQTVRRFRSRFRENVAVLHSQLTAGERLDAWRALHEGRKKVA
                     VGPRSAVFAPVRDVGLIVVDEEHDGSYKQEDSDPRYHARDVAVMRARMSDSVVLLGSA
                     TPSLESLQNVRRGKYTYVRLPGRVDDKPLPEVQIVDMRKERTDGNWGGLSRSLRSAVA
                     RTIENRNQAVILQNRRGFSTVLQCSRCGSIVECQNCQVSMTYHRPEDAMKCHYCGDVR
                     SVPKMCPECNETSLRFSGTGTQKVEDEISKAFPNAILLRMDQDTTHGRNAHHRLLERF
                     RKNEASILLGTQMVAKGLDFPNVTLVGVISADIGLGLPDFRAAERTFQLLTQVAGRTG
                     RGNEPGQVIVQTAMPEDPAILAASRHDVDGFAEDELRKREELGYPPFGKLVLIQLRGS
                     ENDVVRQAARTLAVRASDLAQGKVEVLGPSEAPIPRINKQWRWHILLKGTSSQSLRAI
                     AKGVQESFKRMHSRFVQLSINVDPVSLI"
     gene            complement(2315..3121)
                     /locus_tag="PLT86_01700"
     CDS             complement(2315..3121)
                     /locus_tag="PLT86_01700"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOT35360.1"
                     /translation="MLDTLARREEVYHKEIAQALVKGSAGSADLSGVTLAKTEGIAEI
                     LNYDRYNRWCLIDIFPAPGTTAEEFARVRYVDEGDFSTGSYRWTRQSSSERVEIELVR
                     DGEVRRGSTIWPVRLTKRVEVISGQPGMNIEYVIENHGDRIFEGCFGSEWNFGLLAGW
                     ADDRYYLVNGERGPGDMRLATSGQHSQVREIGVVDHWQRADIRLKTSVPADVWRYPVE
                     TASLSEGGYEKTYQASAVFPHWKVLLEPGTSWMVKVAVSVLEPVWHPPVQ"
     gene            complement(3058..4443)
                     /locus_tag="PLT86_01705"
     CDS             complement(3058..4443)
                     /locus_tag="PLT86_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HOT35361.1"
                     /translation="MPPKTVLMIGLHSHQPVGNFDEVIELAYEQAYVTLLDYLDRHPT
                     VRVNLHYSGCLLEWLERHHPDFLLKIRRVVERGQAEILSGGFFEPILPVIPPADAQAQ
                     LEMLNDFVRDRFGYEPKGFWLAERVWEPVIPSLAVSAGLSYTVLDQSHFVSSGVGKED
                     ANGYYVTEDQGNSVAVFPIDYTMRYTIPWKPVAATLDYLRSKHESEPGRGWTFADDGE
                     KFGSWPETWENIIQKGWFEEFFAGIEASRDWLETKTFSQFMASHPPSGRVYLPTGSYP
                     EMLRWSLPTRVSEPLNRFLEQIEDEEGRDFVRMGFWRNFMAKYPEANNLHKRMLRASK
                     RVSSSVRTEDVVNAARTHVLRSQCNCPYWHGVFGGLYLNFLRHATYAEMLAGEALAAP
                     VEGILSEAYDFDADGQEELIVENARQVLIFSPHAGGALREWDWRDFPRICSTRWHAGK
                     RYTTRRSPRLW"
     gene            complement(4537..5139)
                     /locus_tag="PLT86_01710"
     CDS             complement(4537..5139)
                     /locus_tag="PLT86_01710"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT35362.1"
                     /translation="MAFELPSLPYPDTALEPNYSAKTFSFHHGKHHKAYVDNLNKLIA
                     GSELEGKTLEQIILATANDPAKTGIFNNAAQVWNHTFFWSCMKPAGGGKPSGALASAI
                     DTAFGGYDRFVEQFKAAAVGRFGSGWAWLVLDNGALKIVSTPNGETPMTKGQKALLTV
                     DVWEHAYYLDYQNRRPDFVQTFLDHLVNWDFVAQNFEKAK"
     gene            complement(5307..7112)
                     /locus_tag="PLT86_01715"
     CDS             complement(5307..7112)
                     /locus_tag="PLT86_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT35363.1"
                     /translation="MPQSKQFAPRDVVLEPGLFRDRYELNRRYVMSLKNENLLQNHYL
                     EARIWQTSFRNTHNGETSWGEDRHWGWESPTCQVRGQFLGHWLSAAARIAETGDDSEA
                     RAKLNKVVAELARCQQANGGEWVASIPPSYLEWTAKGQPTWAPHYVVHKTLMGLFDAY
                     AISGNEQALEILSKAAEWFVRWSSKFDPDAMNNILDTETGGMLEVWANLYGVTGKQEH
                     LDLVYRYDRPRLFDRLLNGEDALTNQHANTTIPEAHGAARAWEVTGDERWRRVVEAYW
                     ECAVTNRGYFCTGGQTCGEVWTPPYAFSARLGDKNQEHCTVYNMMRLADYLFRWTGHA
                     QYADYIERNLYNGILAQQNPSTGMVSYFLPLCAGGKKRWGSPTNDFWCCHGTLVQAHT
                     LHNAYGFYKSDDGLILANYIPSSVTWRREGGVVVQARQTIDPEVSAQSLPRRLVVNLV
                     VSCSTQTEFPLQVRVPWWVSGEMEIWVNGVRLDTNAVAPSSFVTIRRTWQQETIRIVI
                     PRSLHSSPIPDMPDMVAFMDGPEVLVGLVHEETRLAGNRDEPGSFLTPDNEREWTTWR
                     RTYRTTRQSRGVRFVPLHAIQDETYTVYFPVEEPR"
     gene            7278..8225
                     /locus_tag="PLT86_01720"
     CDS             7278..8225
                     /locus_tag="PLT86_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35364.1"
                     /translation="MGDRVFEKLADPSLLARSPIFIFAADALTAYGLTGIGIEKTPSP
                     HYRTRPEYLDLQRAILGDGYIDGLLMSPADAENLAFEEKLFDSSPVTPIVRMNAETRI
                     WSPRHGKYGAQPSRPFTTIRTPEAGRYCDEAMACAKDCKIKLGLYSITLNNDTDADER
                     TLSAYLDFAWDVAQTPDFHHFLEVFLPNMSQPGMDLQAMGEYVADSIVRTMAHLRKRQ
                     RPLFVKTVYTTPAVWKALVEFDPTLIIGALGGQRINAKRTLELAHTTTEYGGRVILFG
                     RAIFQEENPRAMCKALRAVLDRQLSPAEAHSAYQRTLRA"
     gene            8259..10214
                     /locus_tag="PLT86_01725"
     CDS             8259..10214
                     /locus_tag="PLT86_01725"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOT35365.1"
                     /translation="MPIPALPTTPYGVIKETWKPWLEALEAWQPSREVVRPHLYQPAG
                     DRNIVRERLSAFPGTIEQYRKAAESALEAEIPSPDKGLRAVGTLIGRATACANWWLLS
                     LDERAARRAWEISLAIMEWPRWVADEHLPLTIDLKSAAAVQALSNVLDRMSDWLQPDQ
                     KATIANCLQDRGVLPFVAIHDSHGEWWTYSLHNWRSVIDGAFGIAAIAVRDTLPTDMV
                     KRALKHSVIGVLTVLDQGDEDGGWYEGVSYWSYGIGEAVQFMDVLQRITEGRVDLFTH
                     PYIGKTGDFCLYETWPDGRVHNWGDCGDRVNAAALMARLAAARQRSDWQAYVRKFPAA
                     PSIESLFWEDPSLQASDLASLPRAKHFRGTQVAVIRSGWADDDVIVGLKAGETTANHS
                     HLDMGSFVINCGRHCLVADAGHWPYAHSLGFFDLDFPDGARWDFPGLATECHSTILMD
                     GQGQQYGEGRDAVVVACADGDDYAYATIDATSAYPNAARFVRYVLLIRPDTIVVVDDL
                     ASPSRCRFGWRVVVPEVPVRGNSIKEWSLTTSDGSASLSIRCLLPGEENGLLAEVSQL
                     AASYPATSTQANPTCQTLTFSNLVRARQATFAVAMRVTRGPLMPPAIEVERIYRGLKI
                     HALDAGKETWAIMWGEPGVRKISTTPG"
     gene            10251..11339
                     /locus_tag="PLT86_01730"
     CDS             10251..11339
                     /locus_tag="PLT86_01730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT35366.1"
                     /translation="MNRIVHQLDPGNENPRNSEGSFVTLTDGTILFAYSRFTTSGSDF
                     GSADIAAIYSHDGGETWSSDPQIIVRNTGGINVMSVSLLRLRDGRIALFYLVKSSEND
                     CRPCMRTSNDEGGTWSAPINVIPTPGYYVVNNDRVVQLKSGRIVIPAAFHRRKTITDT
                     SWKAVDSRGIMLAFLSDDAGATWRESKSWWALPVPGNSGLQEPGCVELTNGQLYGYCR
                     TDTGYQWSTVSRDGGDTWSPPEPAPFISPNSPLSMKRLPPSEPGKNGHLLAIWNDKSP
                     RFASQVRPSSPSARTPLVSAISEDEGRTWAHHRLLESDPESGYCYTAIYMLPNACLLA
                     YCAGSKATGSILNTLRIRKVDYSWFYAT"
     gene            complement(11421..12866)
                     /locus_tag="PLT86_01735"
     CDS             complement(11421..12866)
                     /locus_tag="PLT86_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT35367.1"
                     /translation="MRPWNDTAWKNSMRVRLAMANVFEEAVGSEGGIARARLESLYDR
                     LRAIQREIALKREAGTLPFLDLPKQDVSAILACVEQNRPKFETFVVLGIGGSALGNIA
                     CHTALRHPYHNFLPADRRGGMRIEVADNIDPDRIAGLFDTLDLPRTLFNVITKSGSTA
                     ETASTFLVVKKLLHDHPRIGPGWREHVIVTTDPVAGDLRKFASREGLVSFGIPRGVGG
                     RFSVLTPVGLLSAAMSGIDIEELLAGAADAAEYHASVPAEKCPAFAGAALHYLMYNHP
                     RAPKRLAVMMPYSHALKDVADWFRQLWAESLGKEKALDGTVVNVGPTPIKALGVTDQH
                     SQVQLYIEGPNDKLFTFVETETFQNDLVLPSDNDLSDLGAFNYFAGKTLGQLLHAEQK
                     ATTLALTRRHRPNLTISMREVRPYTVGQLFMLLEIQTVVAGALFGINPFDQPGVEAGK
                     IATYALMDRPGYENEKNAIEQEISTGAEWVL"
     gene            complement(12863..13654)
                     /locus_tag="PLT86_01740"
     CDS             complement(12863..13654)
                     /locus_tag="PLT86_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOT35368.1"
                     /translation="MACDEVLFQLVSNAPWAAILRFYEWKPYAVSVGRSQSPQKEIEL
                     NALRRDGYDCVRRITGGRAVFHVEELTYSVVCAREHPLATGGIHATYERTSEALARGL
                     RLAGIRAEVARVTQHLSPGSPGQSAPPCFGSVARSEIVVDGRKLVGSAQYRSSTAVLQ
                     HGSILTGTAHRRILEYLRVEEDVRQRYLRNLEVGTTCLWELGWRGTTDDLIRALADGF
                     SGHFGVVWESSQGLTPHEMSLIDTRVHERNSRENWNPGDTDGGQG"
     gene            complement(13717..14532)
                     /gene="rnc"
                     /locus_tag="PLT86_01745"
     CDS             complement(13717..14532)
                     /gene="rnc"
                     /locus_tag="PLT86_01745"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT35369.1"
                     /translation="MVWLGRLLSRKGSAAQRTGSAGGIRDLEDAIKYTFRDKSLLIAA
                     LRHRSYVHSNGDSPDDPVSQANERLEFLGDAVLGLLATEFLYREFPDKPEGELTKRKS
                     VLVSKSALARCALEIHLDQHVLLSSVEEGMGGRQRRSILGDAFEALVGAIYLDGGLEP
                     SRSFLETTLFVHLREYETESIFTNYKSLLLEHVQAGGRPPEYMLRSQSGPDHQKTFTV
                     EVAIGGKVLGTGTGTTKKYAQQEAARLAYELLTRRASNGGDQNTDEPFPATGV"
     gene            complement(14580..15830)
                     /gene="fabF"
                     /locus_tag="PLT86_01750"
     CDS             complement(14580..15830)
                     /gene="fabF"
                     /locus_tag="PLT86_01750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT35370.1"
                     /translation="MGGKTRRVVVTGMGAVTPVGNSLEEYWQALLHGKSGAGSITRFP
                     TEGFSTTIAAEVKGFDPTQRISAKEARRMDLFIQYAVYAASEAIEDADLRVDGMDPTR
                     VGVIVGSGIGGISTLEAQYKVLKERGPGRVSPFMIPMLISDMAAGMISIIFGLKGPNY
                     CTVSACASAAHALGDAVSVLRNGHADVVLTGGAEASLTELAMAGFCAARALSTRNDDP
                     EHASRPFDKDRDGFVCGEGAGILVLETLDHAIARGARIYAEVLGFGYSADAYHITAPP
                     ENGEGMARCMENALLDAEMPKERVGYINAHGTSTEVGDAAETAGIKNVFGEHAYRLAV
                     SSTKSMTGHLLGAAGAVESIATILALHHGVLPPTINLSTPDPACDLDYVPNSPREKRV
                     DVAMSNSFGFGGHNASLVFGSWKD"
     gene            complement(15885..16121)
                     /gene="acpP"
                     /locus_tag="PLT86_01755"
     CDS             complement(15885..16121)
                     /gene="acpP"
                     /locus_tag="PLT86_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT35371.1"
                     /translation="MAIEDKVRDLVVEQLGVAPEEVKPEARFIEDLGADSLDTVELVM
                     AFEDKFGVEIPDEEAEKLDTVGAAIEYLKSKNVE"
     gene            complement(16247..16993)
                     /gene="fabG"
                     /locus_tag="PLT86_01760"
     CDS             complement(16247..16993)
                     /gene="fabG"
                     /locus_tag="PLT86_01760"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT35372.1"
                     /translation="MFDLNGRVVLVTGGSGDIGAALCEKFALAGAKVAMTSRTGSAGL
                     ARVLETLRAHGAETLPISMDVRQRSDVEAGVAKVVETWGRLDVLVNNAGITKDTLLLR
                     MNDQQWNDVLETNLTSVFLCTQAALKPMLKARWGRIISITSIVGEVGNAGQANYASAK
                     AGIIGFTKTVAKECASRNITVNAIAPGFIESRMTDTIPEKVRATLLASIPLGRPGTPM
                     EVAAAAVFLASEEASYITGHVLNVDGGIAM"
     gene            complement(17010..17927)
                     /gene="fabD"
                     /locus_tag="PLT86_01765"
     CDS             complement(17010..17927)
                     /gene="fabD"
                     /locus_tag="PLT86_01765"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT35373.1"
                     /translation="MGIAFLFPGQGSQYVGMGADLFAEFPAARRTYEDADALLGWPVT
                     SMSFAGPAETLNDTRYTQPAVFVHSMAVFEILRQHGIRPDVVAGHSLGEYAALCASGA
                     LEFGAALRLVAARAAAMADVASRKPGTMAAVVGLDERTVEKSVQGLRDVVVANYNAPD
                     QVVISGAEEAVGNAGTALSELGAKRIVPLKVSGAFHSPLMAEAARVFADALEREEIST
                     PGIPVIPNVTAKRTTDPEEIRSLLVEQITSPVRWTDTVRSLASSGSASCFEVGPGRVL
                     QGLVRRIAPSITTTTVGTTAEIRALLKTQ"
     gene            complement(17939..18922)
                     /locus_tag="PLT86_01770"
     CDS             complement(17939..18922)
                     /locus_tag="PLT86_01770"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOT35374.1"
                     /translation="MRHPERAFFKGLGAAAPTKVLSNHDIEKLVDTSDEWIRTRTGIE
                     RRHVADDDTNTLTLATEAGRKALFDAGVEPEEVDMIILGTLTPYIGFPATACLVQNAL
                     GARNAAAVDINAACSGFLYGLHLAQSLICSGANRNVLLIGAETMSRIVNWKDRNTCVL
                     FGDAAGAALLAPSAGNKDGAGVLGTYIRSDGVQPEMLCRIGGGTKPVAKEEENYLQMS
                     GKDVFKRAVTAMGDAAEQILRQTGFSGEDITLLIPHQANMRIIEATAKRVNIPMDKVV
                     VNIQEYGNTSAATIPVAMAEAKASGRMRSGDIVLMVAFGAGMTWGAALARM"
     gene            complement(18909..19928)
                     /gene="plsX"
                     /locus_tag="PLT86_01775"
     CDS             complement(18909..19928)
                     /gene="plsX"
                     /locus_tag="PLT86_01775"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOT35375.1"
                     /translation="MERVCTIAVDAMGGDFAPDEIVAGAVHAAQQGDGAIRVILVGQE
                     QAVKASLARFGEVPGVEIRHAPTTVGMAESVSNLRRKMDSSLAVAIKMQKTGEADAAV
                     SAGNTGAAMALSLLLLGRIPGVNRPAIASPMPTKKGVCTLLDVGANSSCEPLNLYQFA
                     VMGSLYHSFVYNVPNPSVGLLSIGEEASKGDELVQKTHSLLAASSLNFVGNVEGRDIL
                     SGTSDIVVCDGFTGNVVLKSAESAVQFITGVLKDAIKSSPLSMLGGLLIKRQFNKQKT
                     RLNYEEYGGAPLLGIDGITIICHGRSSARAIQNAIRVAATAARQKLNEHIKNKLSEAS
                     IDATS"
     gene            complement(20037..20219)
                     /gene="rpmF"
                     /locus_tag="PLT86_01780"
     CDS             complement(20037..20219)
                     /gene="rpmF"
                     /locus_tag="PLT86_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT35376.1"
                     /translation="MANPKHRTSKARTRSRRTHWKLTAPNVVKCSHCGAPKVAHRVCP
                     ECGYYGQRLAVAPREE"
     gene            complement(20274..20744)
                     /locus_tag="PLT86_01785"
     CDS             complement(20274..20744)
                     /locus_tag="PLT86_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOT35377.1"
                     /translation="MVIDFSTFQDGIHAVDWAGEIPGVDLIYPEIVAAIQVRAKIHRL
                     QEVVTIRAALRAQLERPCDRCLRPAVLAIESSLYVVVKLRAGLSAAEMGDDGEVLITV
                     DREESQVDLTDHIRESLIVEVPMTILCDDDCKGICPRCGADLNEESCSCRRQAE"
     gene            20937..21506
                     /locus_tag="PLT86_01790"
     CDS             20937..21506
                     /locus_tag="PLT86_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT35378.1"
                     /translation="MLKDLVRECRSTRRFKQHIPVTRDALVEMVDLARICASGSNKQA
                     LKYFVSADPEQNARIFPTLAWAGYLTDWPGPAEGERPAAYIIILGDTTISEGFGVDHG
                     IAAQTIALAAREKGLGTCIMGAIQRPKLQEILNIPSHLQILLVLAIGVPAEKIQLEPV
                     GPDRSIKYYRDENGVHHVPKRSLEEVLVN"
     gene            complement(21510..23762)
                     /gene="rnr"
                     /locus_tag="PLT86_01795"
     CDS             complement(21510..23762)
                     /gene="rnr"
                     /locus_tag="PLT86_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOT35379.1"
                     /translation="MRTGSRKPARRAHLPPGEAILAALSARAKPLSLQQLASAVHSTQ
                     DDPLFRQAVMDLRREGKIYEGRGQRYASASGLGARLGTLRLAERGYGFVAVEGREDDL
                     YIPAEDIGNALDGDTVLVVEQSPRGRRNLGRGVVLGIAYRPERMIVGTFHKGVGFAWI
                     RPFSAQLPDLQLVFLGAEGEEKAPSEGDVITAVLEPLSLESSAPAARFGTALGKQGDP
                     WVDVTAILHAHGIRLSFPAEAVREAQQVAERAAEEAGKRADLRGLETVTIDPSDAADI
                     DDAISLSKDDAGRDVVGIHIADVSHFVPEKGAIDKEAYLRGTSVYLPGKVVHMLPPQL
                     AAGLCSLLPRQDRLAVSVLITLDENVLPVRVEILESVIRSCAKISYEEAQEALGGSAR
                     PDNPAFPHAGMLQRLADITGELARKRSRHGALDFDVPEVRVEVDAQGRVVALRRRERL
                     DSHRVIEELMLLANSVIADHLSQIHYPLLYRVHAGPDPAKLQELAPLALSLGQVIAPD
                     GRSPSVQDLQKLLDAIRGNPVAPVLEALIIRSLPKAVYQPDNIGHFGLAMDAYTHFTS
                     PIRRYPDLVVHRQLKRALNGTPPCYSHEQLFTIGDHCSAAERTAETAEREAIRAKQAE
                     YLAGRVGEEFDGTVSGVRKFGLFVVLNDSLAEGFVPVDDLPADTYAFDPTSLTLEGRR
                     SRMTFRFGDPVSVQVVRVDVGSRRIDFLLVEAPHSHRTRGPRTKTLRRSSKRPSRPTP
                     GKRRGGRPSR"
     gene            complement(23769..24542)
                     /gene="purQ"
                     /locus_tag="PLT86_01800"
     CDS             complement(23769..24542)
                     /gene="purQ"
                     /locus_tag="PLT86_01800"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOT35380.1"
                     /translation="MRPHVLVLRAAGSNCDVETAHAFSVAGGEAERIHVNRVAEKPSL
                     LEETSILVIPGGFTYGDDVSAGKVLANELMTRLSEPLLRFVERGGLVLGICNGFQVLV
                     KTGLLPGWNEQTPSCTLAGNLSGKFEDRWVWLKPGSSRCVYTRGIEKPIYLPVAHAEG
                     RFTASSLEVLDRLETSDQVVFRYTDALGNDVGYPGNPNGADRSIAAICNVSGRVMGMM
                     PHPERHIHPTHHPQWTRNASRSRPDGLAIFENAIRAAQE"
     gene            complement(24563..25438)
                     /locus_tag="PLT86_01805"
     CDS             complement(24563..25438)
                     /locus_tag="PLT86_01805"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT35381.1"
                     /translation="MYPELFKIGPVTVYSYGAMLACAFIVGAWLFRRELRRWNLPEDL
                     ADRVALAAMFGGIIGAKLYFIALEAPDEFAAEPLNTLFSGAGLTFYGGLAGAVLFIWF
                     VVRRAKVSFLHVADLLGPILVLGYAFGRAGCFLSGDGDYGPPTNLPWGMSFPNGTVPT
                     VLMVHPTPLYEIGLCLVGFGYLWFVLRHKTLAPGMMVAATFLVAGVERLITEFWRFDS
                     GVYAMYAYAHGGTSLAESQRVAFEIARKAHFAYAGLSLAQVLSVALVIAGVVILFVQR
                     RKPARGIPGAVGRKS"
     gene            25697..26698
                     /locus_tag="PLT86_01810"
     CDS             25697..26698
                     /locus_tag="PLT86_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOT35382.1"
                     /translation="MPLLTMKQILDDAIAKNYGVGAFNINNMEQTLAIMSAADRTESP
                     VILQASRGARKYAGPVMIYGLVQAAMKLYPKVPVALHLDHGNNFETCKQSMDDGWTSV
                     MMDGSLMEDGKTPSTFEYNVDVTSRVVAEGHKRGITVEGEIGTLGGIEDGHGADRVKL
                     ADPDQAVEFAHKTGVDALALAFGTSHGAFKFKAKPVLAYDIVQAVNKKMPGLAIVSHG
                     SSSVPPELVAEINKYGGKMPGAMGVPLEDIVRSIDFGVRKINIDTDLRLAATATIRKV
                     FVETPEKFDIRDYLRPAMEAMSHVVETRMIAFRTAGKASNFTKVISLEEMAKVYAKK"
     gene            complement(26788..27750)
                     /locus_tag="PLT86_01815"
     CDS             complement(26788..27750)
                     /locus_tag="PLT86_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35383.1"
                     /translation="MERVRLGLIGAGGMANAVHYPSLAEMPDVEMTALCDLDEERLRK
                     TADRFRIAARYTNYRSMLDNEQVDAVYILMPPHQLFEPVMDAIERGKHVFIEKPPAVT
                     SDQCLQMATAAKNRGVHTMVAFNRRFIPVMQKAREMVTAKGDVIQVVSTFYKDYRGQP
                     PYYRGAVDILTSDMIHAVDMLRWMAGAELVDLVSDVQSRNASYANMFNALVRFENGCV
                     GALLGNWNVGGRRHVFEMHADGISAYCNPDAAVEILADNGQPVVFSSKEVAGSEENRV
                     YYGFAAENRHFIDSLKADREPETSFGDAHKTMLLVDLIYRNAWN"
     gene            complement(27836..28861)
                     /locus_tag="PLT86_01820"
     CDS             complement(27836..28861)
                     /locus_tag="PLT86_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOT35384.1"
                     /translation="MATDLNAISRVIRSKNRFVVSAHMHFDGDAGASQLAMARLLRMA
                     GKEAIVVNDEETPIQFRFLPEIETILTAVPADYAPDAVVIIDTPVRENINCGDIRRRF
                     QKPVVPAEEAGTIGKSAWKDVVTIYLDHHERSDPLGEYAYVDPTASAAAVIVARLLKA
                     LKVPADSTLGTMIVCGIMSDTGRFSFANTTPEALRIVADMIDAGASVNQVATELYYKN
                     SFDSVRVTGQALSTMELSASGKVCSMLIYEEPVNAQGQAIEVEDLPNWPVTIRGVEIG
                     MFLRPGADGTVRVSIRSAGDADVNKFASQFGGGGHKKAAGCKMKIPIEEARRALVAAA
                     EKFLLEK"
     gene            complement(29022..29867)
                     /locus_tag="PLT86_01825"
     CDS             complement(29022..29867)
                     /locus_tag="PLT86_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOT35385.1"
                     /translation="MFDYHHHSEFSVDCTASMADICRVAVERGVSEIAFTEHMDFIPE
                     EKNTGYFRYDAYMESIRRCREQFRDELAIVAGIEVDYCPDFENETAKWVEEREFDFVI
                     GSVHYLRGKGNISEPRALDFFRGKTEREAYGEYWDVVLRCARFGIWDTLGHIDVVKRY
                     GTQVFGPFDETRHAEALGEILSTLVTKRIALEINTSGLRQPSAECYPSAALLRRYREL
                     GGERITVGSDSHSVEHTGAGVLFGLRMARETGFAHVERFRRRKRSAVPINLEFEPGNE
                     RLLQQ"
     gene            complement(29914..>30566)
                     /locus_tag="PLT86_01830"
     CDS             complement(29914..>30566)
                     /locus_tag="PLT86_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35386.1"
                     /translation="GNSRSQPVTGLLLEGIVASGKTSVIRAIQALPAWQRRSSKLLLS
                     EFFTERANEHLRNRDADAYRALMHRNLRILDAARQIEMASPLLSEESGRELCYLLERF
                     HFTNAISYADGLFETYRDIDLTLARLNCRVVVLMVEESMVSSRIAEAFRQRGQAWQAY
                     QKRLEEKVGDLGTHYVGVQRAFVSGLNWTSMEHRTIDATDKDWRRCAEEILEFWRI"
BASE COUNT         6382 a         9380 c         8347 g         6457 t
ORIGIN      
        1 gccgtccacg agaacggttc tgagcccgcc caggttgtcc tgagacccgt agctgatttc
       61 cccgaccctt ctccgtccct cgacctcacc atgctttctc tcactcgctg gatagccgat
      121 tactatctgt cgagctgggg agaggccata cgagcggcga ttcctcacgg tctcggaaca
      181 aacagtgtat ggatggttca cctttcgtgc gagaaccctc acgtggtcgc ggataatctc
      241 caggcgcgct tcccaaaaag ggcggaaatc ctgcgttatg tcgccgaaaa aggtcaggtg
      301 tcattaaaaa ctctcgcccg cgtgttttcg gggaccggct ttcgctcgaa tcttcaggct
      361 ttgcagaatg acggacatgt caaagtcaga gacgaagcgg agccaatcgt tctttcccgg
      421 gtccggacgg aaaagctggt ccaactggct cccgcgtatc tggatccgga tgcaactcgc
      481 cgtatgcgtt ttggggctga acaactccgg gttatcgacg gtttgcggac gatttctgag
      541 ccgctttccg tcgcgagtgt ccgatctcgg ttcggagttt ctaccgccac ggtgaaccgt
      601 cttgttgcgc ggggagtcct taacgtagag gagcacgagg ttgaccggga acctgactat
      661 ggcgaatgcg aagacgaaac tggacaactt ctgaccaacg accagaggga cgcgctcgga
      721 gtcattctgc cgaacgtggc ctcccggaaa ttctccgcgg ttctgctgca cggcgttacc
      781 ggcagtggca aaacgcgggt gtacctcgac gcaattgaac acacgcttga cgcgggccgc
      841 ggtgccattg ttctggtccc cgaaatcagt ctgacgccgc agacggtacg gcgatttcgg
      901 tcacggttcc gggaaaacgt cgcggtcttg cacagtcagt tgactgccgg cgaacggctt
      961 gatgcgtggc gggcgctcca cgaaggaaga aagaaggtgg cagtaggccc caggtcggca
     1021 gtattcgcgc ccgtacgtga tgtcgggttg atagttgtgg acgaggagca cgacgggagt
     1081 tacaaacagg aggattctga tccgaggtac cacgcgaggg acgtcgccgt catgcgcgca
     1141 cgaatgagcg acagcgtcgt gttgcttggt tccgcgacgc cctccctgga atcgctccag
     1201 aacgtgcgtc gagggaagta cacctacgtt cgccttccgg ggagggttga tgacaaaccg
     1261 ctgccggaag ttcagattgt ggacatgcgg aaagagcgca cagacggtaa ctggggcggt
     1321 ctctcccggt cgctccgttc cgccgttgcc agaactatcg agaacaggaa ccaggcagtc
     1381 atccttcaga acaggcgcgg cttctcaacg gtgctgcaat gttccaggtg cggttcaatc
     1441 gtagaatgcc agaactgcca ggtgtcgatg acatatcacc gtccggaaga cgccatgaaa
     1501 tgtcactatt gcggcgatgt tcggtccgtc ccgaagatgt gtccggaatg caacgaaact
     1561 tcgttacgat tctcgggaac aggtacgcag aaagtggagg acgaaatctc caaagccttc
     1621 ccgaacgcga ttctgcttcg catggatcag gacacaaccc acgggagaaa cgcgcaccac
     1681 agattactgg agagattccg aaaaaacgag gcttcaatcc tcctcggaac ccaaatggtc
     1741 gcgaaggggc tcgacttccc gaacgttacc cttgtggggg tgatctctgc agacattgga
     1801 ctgggactgc ctgattttcg ggccgccgaa agaacgtttc aactcctgac tcaggttgca
     1861 ggcaggacgg gtcgcggaaa cgaacccgga caggtgatag tccagacggc gatgccggag
     1921 gatccggcga ttctggcggc gagtcggcat gatgttgatg gttttgcgga agacgagttg
     1981 cgaaagcggg aagagctggg gtacccgccc tttgggaagc ttgtgctgat acagctccgc
     2041 ggttcagaaa acgatgtcgt ccgtcaagcc gctcgcacac tcgcggtgag agcgagcgac
     2101 ctcgcacaag gaaaggtcga agtccttgga ccgtcagagg cgcccattcc aagaatcaac
     2161 aagcaatggc gctggcacat actcctgaaa gggacatcaa gtcagagtct ccgcgctatc
     2221 gcgaaaggag tccaagaatc attcaagcgt atgcattcac gtttcgtgca actttccata
     2281 aatgtggatc ccgtatctct gatctagacg cgcttcactg caccggtggg tgccagactg
     2341 gctccaggac cgacacggca accttcacca tccacgatgt gcccggttcg agaagaacct
     2401 tccagtgggg aaacaccgcc gacgcctggt acgttttttc gtacccacct tccgagagag
     2461 acgctgtctc aacgggataa cgccacacgt cggccggaac agaagttttc aagcggatgt
     2521 cggcgcgttg ccagtggtcg acaactccga tctcccttac ctgcgaatgc tgtcccgaag
     2581 tcgcgagccg catatccccg gggccgcgtt cgccgttcac caggtaatac ctgtcatccg
     2641 cccaccctgc gagaagaccg aaattccatt cagagccaaa gcaaccctcg aatatgcgat
     2701 caccatggtt ctcgatgacg tactcgatat tcattcccgg ctgtcccgag atcacctcga
     2761 cccgctttgt caacctgaca ggccagatgg tgctcccccg tctcacttcg ccatcccgca
     2821 ccagctcgat ctctacgcgt tcactggacg attgccgagt ccatcggtat gatccggtgg
     2881 agaagtctcc ctcgtctacg taccggacac gtgcgaactc ttcggcagtg gttcctggag
     2941 caggaaaaat gtcgatcagg caccaccgat tgtaccgatc gtagttgaga atctcagcga
     3001 taccttccgt tttcgccagc gtgacacccg aaaggtcagc cgatccggcg cttcccttca
     3061 ccagagcctg ggcgatctcc ttgtggtata cctcttccct gcgtgccagc gtgtcgagca
     3121 aattcgtggg aaatcacgcc agtcccattc ccgcagggcg ccacccgcat gcggcgagaa
     3181 aatcagaact tgccgggcgt tctccacgat gagttcctcc tgtccgtcag cgtcgaagtc
     3241 gtacgcttcc gacaggatac cttccaccgg tgcggcgaga gcttcaccgg ccaacatctc
     3301 cgcgtaagtg gcgtgtcgca gaaaattcag gtagagccct ccgaatacgc cgtgccagta
     3361 cgggcagttg cattgcgagc gaagtacgtg agtgcgggcg gcgttgacaa cgtcttccgt
     3421 tcgcacggaa ctcgagacac gcttgctggc ccgcaacatg cgtttgtgga ggttgttggc
     3481 ttctgggtat ttcgccatga aattccgcca gaaacccatc ctcacgaagt ctctgccctc
     3541 ttcatcttca atctgttcga gaaaacggtt caagggttcg gatacccggg taggaagcga
     3601 ccaccgcagc atctccgggt aacttccggt cggaaggtaa accctccccg aagggggatg
     3661 ggaagccatg aattggctga acgtcttggt ctccagccag tcgcgcgagg cttctatgcc
     3721 cgcgaaaaac tcttcgaacc accccttctg aatgatgttt tcccaggttt ctggccatga
     3781 gccgaatttc tcgccgtcat ccgcgaaagt ccagcctctt ccgggttcag attcgtgctt
     3841 cgagcggagg taatcaaggg ttgcggcgac cggtttccag gggatggtgt accgcatcgt
     3901 gtaatctatg ggaaatactg ctaccgaatt tccctgatcc tcggtaacgt aatacccgtt
     3961 ggcatcctct ttccccactc cgcttgacac aaaatggctc tggtccagca ctgtgtagga
     4021 tagccccgcg ctgactgcaa gagacggaat cacgggttcc cagactctct ccgcaagcca
     4081 gaaccccttc ggttcgtacc cgaagcgatc acgtacgaaa tcgttcaaca tttcgagctg
     4141 cgcctgggca tccgccggtg gaattaccgg taggataggc tcaaagaaac ctccggaaag
     4201 aatctcagcc tgcccgcgct cgaccacccg gcggattttc aataggaaat ccgggtgatg
     4261 cctttccagc cactccagca agcacccgct gtaatgcagg ttcaccctca ccgtcgggtg
     4321 gcggtcgagg tagtccagca acgtcacgta tgcttgttca tacgcgagtt cgatcacctc
     4381 gtcgaagttg ccgacgggtt ggtgactgtg caggccgatc ataagaacag tttttggggg
     4441 cacgctcgcc tcctctcggt tatcttgggg cttagggaaa acgaaagcgc cgtgcagaag
     4501 gttctcgcac ggcgctcatt tccgaccaca ggggtttcat ttcgcctttt cgaagttctg
     4561 agcgacgaaa tcccagttta cgaggtggtc aaggaaggtc tgcacaaaat cgggccggcg
     4621 gttctggtaa tccaggtaat acgcgtgctc ccagacatcc accgtaagca aggccttctg
     4681 tccctttgtc atgggtgtct caccgttagg cgtacttacg attttcagcg cgccattatc
     4741 gagaaccagc cacgcccagc cgcttccgaa tctccccact gcggctgcct tgaattgctc
     4801 cacaaatctg tcgtacccgc cgaacgccgt gtcaatggcc gaagcaaggg ctcccgaagg
     4861 ctttcctccg cccgccggtt tcatgcagct ccagaaaaac gtgtggttcc agacctgggc
     4921 ggcgttgttg aaaattccgg ttttcgcagg gtcattcgcg gtggccagta tgatctgttc
     4981 cagcgttttt ccttccagtt ccgacccggc tatcagcttg ttcagattgt caacatacgc
     5041 tttgtgatgt ttgccgtggt ggaaactgaa cgtttttgcc gaataattgg gttccagtgc
     5101 ggtgtctggg tacggaagcg aaggaagttc gaatgccatg gcgccctcct ttgtggtgaa
     5161 aacatggacg gacttgagaa tgaaactgtt aatttaacgg caaaaccatg caaatgagtt
     5221 ccatctcttt ccgccccgaa tgtgatgcgt cgccacatgg tattcgccgc ggctgtcgtc
     5281 acggatgtca tccgaacata accacatcac cggggttctt ccactgggaa atacacggta
     5341 tacgtttcat cctgtatcgc atggagcggc acaaatcgca cgccgcgtga ttgccgtgtc
     5401 gttcggtagg ttcgtcgcca tgtcgtccat tcgcgctcgt tatccggtgt cagaaagctg
     5461 ccgggttcat ctctgtttcc ggcgaggcgc gtttcttcgt gaacaagccc cacaaggact
     5521 tcgggaccat ccatgaatgc caccatatcc ggcatgtccg gaatcgggga agagtggagg
     5581 ctcctgggaa taacaattcg gatcgtctcc tgctgccacg ttctgcggat tgtcacgaac
     5641 gaagaaggcg caacagcgtt tgtgtccaga cgcacaccgt tcacccagat ctccatttct
     5701 cccgacaccc accagggcac gcgcacctgc aaggggaact cggtctgtgt tgaacaggac
     5761 accacgaggt tcaccaccag gcgccttggc aacgactggg cggacacctc cggatcaata
     5821 gtctgtcggg cctgtacgac aacaccaccc tccctccgcc aggttacact ggacgggatg
     5881 tagttcgcga gaatcaaccc gtcgtcagat ttgtaaaagc catacgcgtt atgcaacgtg
     5941 tgagcctgca ccagtgtgcc gtggcaacac cagaagtcat ttgtgggtga tccccagcgc
     6001 ttcttcccac ccgcacacag cgggaggaag tacgacacca tccccgtcga cgggttctgc
     6061 tgcgcgagga taccgttgta cagattgcgc tcgatgtagt ccgcgtactg cgcatgtcca
     6121 gtccagcgaa acagataatc cgcaagccgc atcatgttgt agacggtgca atgttcttgg
     6181 tttttgtccc ccaaccgcgc ggaaaacgca taaggcggcg tccacacctc accgcacgtt
     6241 tgtccccccg tgcaaaagta tcctctgttc gtcactgcgc attcccagta cgcctctaca
     6301 acccgtctcc accgctcatc gccggtgact tcccacgcgc gtgcagcgcc gtgagcttcg
     6361 gggatggtcg tgttggcatg ctgatttgtg agagcgtctt ccccgttcag aagccggtca
     6421 aaaagccgtg gacgatcgta tctgtacacc agatcaagat gctcctgttt gcctgtgaca
     6481 ccgtacagat tcgcccagac ttcgagcatt ccgcctgttt ctgtgtcaag aatgttgttc
     6541 atcgcgtcag gatcgaattt gctcgaccag cgaacgaacc attccgcggc tttcgaaagg
     6601 atctccagag cctgctcgtt ccccgaaatg gcatacgcgt caaaaagacc catcaaggtc
     6661 ttatgaacca cgtagtgcgg tgcccaggtg ggctgacctt ttgcggtcca ttcgagatat
     6721 gacgggggta tggaggccac ccactctcca ccatttgcct gttggcatct tgcgagttcc
     6781 gccaccacct tgttcagctt tgcgcgtgct tcagagtcgt cgccagtttc ggcgattctg
     6841 gcggccgcgg atagccagtg cccgaggaac tgcccgcgaa cctgacacgt cggggattcc
     6901 catccccagt gccggtcttc tccccagctt gtctctccgt tatgcgtgtt gcggaagctg
     6961 gtctgccaga tccgcgcttc cagatagtgg ttctgcaaaa ggttctcgtt cttcaaactc
     7021 atcacgtacc gccgattcag ctcgtagcgg tcgcgaaaaa gccctggttc cagtaccaca
     7081 tcacgcggtg caaactgctt cgactgcggc atcatccgtc ctccaaacgt ggctctctcg
     7141 tgtctgcaaa aatgaggtta aggacggcca tgccgtccgc acaacgggtc cgcggcgggt
     7201 tgacttcgca tgccaggtct cgtatttctg aagcgtgcaa acagagtcgg tcgaaccttt
     7261 gatcaggaag gcacgctatg ggcgacaggg tttttgagaa actggcagat ccttctcttc
     7321 tcgcacggtc tccgattttt attttcgccg ccgacgcact gaccgcatac ggtcttaccg
     7381 gaatcgggat agagaaaacg ccgtcacctc attatcgcac gcgtccggag tatctcgatc
     7441 ttcagcgagc tattctcggg gacggatata tcgacggttt gttgatgtca cctgctgacg
     7501 cggagaacct ggcgtttgaa gaaaagttgt ttgattcgtc gcccgtaaca ccaatcgtac
     7561 gcatgaacgc agaaacgcgt atctggagtc ctcgccacgg taagtacggt gcccagccct
     7621 cgcgtccttt taccacgatt cgtacgccgg aagcgggcag atactgtgat gaggcgatgg
     7681 cctgcgcaaa ggattgcaag atcaaactcg ggctctactc aatcaccctc aacaacgata
     7741 ccgatgccga cgaacgaacc ctgagcgcct acctcgattt cgcgtgggac gttgcgcaga
     7801 ctccggattt ccatcatttc ctcgaagtgt tcttgccgaa catgagccaa cccggtatgg
     7861 atctgcaggc catgggcgaa tacgtcgcag actccatcgt gaggacgatg gcgcatctgc
     7921 ggaaacgcca aaggccgcta ttcgtaaaaa ctgtgtatac cacaccggcg gtctggaagg
     7981 cgctggtgga gttcgacccc accctgatta tcggcgcact cggtggccag cgcatcaacg
     8041 cgaagcggac ccttgaactc gcgcatacca ccacggaata cggtggcagg gtcattctgt
     8101 tcgggcgggc gatttttcag gaggaaaacc cgcgcgcgat gtgcaaagcg cttcgggccg
     8161 tgctcgaccg gcaattgtca cccgccgagg cgcattcggc ttaccagcgt acgctcagag
     8221 cgtgacctcc cccattcccc gatattgagg acgccaacgt gcccattccc gcactcccca
     8281 ctactccgta cggggtaatc aaagaaacct ggaagccctg gctggaagcg ctcgaggcct
     8341 ggcaaccgtc tcgagaagtc gtgcgtccgc acctgtacca accggcgggc gatcgaaaca
     8401 tcgttcggga aaggctttcc gcattcccgg ggacgattga gcaatacaga aaagccgcag
     8461 aatcagcgct cgaagcggaa atcccttcgc cggacaaagg tctgcgggcg gtcggaaccc
     8521 ttatcggccg ggccaccgcg tgcgcgaact ggtggttatt gtcgctggat gagcgagcgg
     8581 cgcggcgtgc gtgggaaatc tcccttgcga tcatggaatg gccccgatgg gttgccgacg
     8641 aacaccttcc cttgacgata gacctgaaga gcgcggctgc ggtccaggct ttgagcaatg
     8701 tgcttgacag gatgtctgat tggctccaac cggatcagaa agccactatc gcaaactgcc
     8761 tgcaagatcg cggcgttctg ccctttgttg caatccatga ttcacacggg gaatggtgga
     8821 cgtacagcct gcacaactgg cgatccgtca ttgacggcgc attcggaatc gcggcgattg
     8881 ctgtacgcga caccctgccg acagacatgg tgaagcgggc gctaaaacat tccgtgatag
     8941 gcgtcctcac ggttctcgac cagggcgacg aggatggcgg gtggtatgaa ggggtatcct
     9001 actggagcta cggtatcggt gaggccgttc agttcatgga tgtgcttcag cgtataactg
     9061 agggaagggt ggatttgttc acacacccct atatcggaaa gacgggcgac ttctgcctgt
     9121 atgaaacctg gccggacggt cgcgtgcaca actggggaga ctgcggtgat cgcgtgaacg
     9181 ccgccgcatt gatggcgagg ttggcagccg ccaggcaacg cagcgactgg caggcgtacg
     9241 tgcgcaagtt cccggcggcg ccgtccatcg agtcgctctt ttgggaagac ccttccctgc
     9301 aggcttcaga tttggcgtcg cttccccgtg caaaacactt tcgcggaacg caagtagccg
     9361 tcattcgttc cgggtgggcg gatgacgatg tgatcgtcgg cctcaaagcc ggcgaaacga
     9421 cggcaaacca ttcccatctc gatatgggat cgtttgtcat taactgcggc agacactgtc
     9481 ttgtcgcgga cgcaggccat tggccttacg cgcattccct aggttttttt gatctcgatt
     9541 tccccgacgg cgcccgctgg gactttccgg gattggcaac ggagtgtcac agcacgatcc
     9601 tcatggatgg gcaagggcag cagtacggcg aaggccgtga tgcagttgtt gtcgcgtgcg
     9661 cggatggcga cgactacgcg tatgccacga tagacgcaac gagcgcatat ccgaacgcag
     9721 cccggtttgt gcgctatgtg ctgttgatcc gtcccgacac gatcgttgtc gttgatgacc
     9781 tcgcgtctcc ttcgagatgc agattcggct ggcgggtggt ggtgccggaa gttcccgtgc
     9841 gtggaaactc catcaaagag tggtccctga cgacgtcgga cggctcagct tcgctctcaa
     9901 ttcggtgtct gttgccggga gaggagaacg gcctcctcgc cgaagtgagc caacttgcgg
     9961 cgtcttaccc ggcgacgagt actcaggcga atcccacctg ccagaccctg acgttcagca
    10021 accttgtacg agcccgtcag gcaaccttcg cggtggccat gcgggtaacc cgcggccctt
    10081 tgatgcctcc ggcaattgag gttgaacgga tctaccgagg gctgaaaatc cacgcccttg
    10141 atgccgggaa agaaacgtgg gcaatcatgt ggggagaacc aggagtccgg aaaatcagca
    10201 ccacgcccgg ttgacgtgcc gcaatctcat acaccaaagg agctcgggct ttgaacagaa
    10261 tagtccatca acttgacccc ggcaacgaaa atccgagaaa ctccgaaggc agtttcgtta
    10321 cgttaacaga cggaacgatt ttgttcgcct acagccgatt caccacttcc ggcagcgatt
    10381 ttgggagcgc cgacatcgcc gcgatctact cacacgacgg cggggagact tggtcgagcg
    10441 acccgcagat catagtcagg aacaccggcg ggatcaacgt gatgagcgtt tccctccttc
    10501 gcctgcggga cggcagaatc gctcttttct acctcgtcaa gagctctgaa aacgattgcc
    10561 gtccctgtat gagaacgtca aatgacgaag ggggtacatg gagtgctcca atcaacgtga
    10621 tccccacgcc aggatattac gtggtgaaca acgaccgcgt cgtgcagctc aaatcggggc
    10681 ggattgtcat acctgcggcg tttcaccggc ggaaaactat caccgacacg tcgtggaaag
    10741 ccgtggattc gcggggcatc atgctggcct tcctatcaga tgatgcggga gccacatggc
    10801 gggaaagcaa atcctggtgg gccctgcccg tgcccggcaa tagcgggttg caggaacccg
    10861 ggtgcgtgga actcacgaat gggcagctgt acggctactg ccggacggac acggggtacc
    10921 agtggtcaac cgtatcacgg gatggcggag acacgtggtc cccacctgaa ccagctccat
    10981 tcatttcgcc aaattcgccc ctttccatga aacgactccc tccttccgag ccgggcaaga
    11041 acgggcatct gctcgcaatc tggaacgaca aatccccgag attcgcatca caggtgcggc
    11101 ccagcagccc cagcgctcga acccctttgg taagcgcgat aagcgaggat gagggacgaa
    11161 cgtgggcgca tcatcggctt ctcgaaagcg acccggaaag cgggtactgc tacaccgcca
    11221 tatacatgct cccaaacgcc tgtctgttag cctattgcgc ggggtcgaag gcgacgggca
    11281 gtattctcaa cactctccgc attcgcaaag ttgattattc ctggttctat gccacgtaga
    11341 tttcagccag aagaccacgc accgtgtgtg cgttctgagt gcgttttccg cggcggagaa
    11401 tgaacctcta agaccgcacg tcacagcacc cactccgctc ctgttgaaat ctcctgctct
    11461 atggcgttct tctcgttttc gtatccgggc cgatccatga gcgcgtaggt ggcaattttg
    11521 cccgcctcca caccgggttg gtcaaacggg ttgattccaa acagcgcccc cgcaacaacc
    11581 gtctgaatct ccagaagcat gaacagctgg cctactgtgt acggtcgaac ctcccgcatc
    11641 gagatggtca ggttcggtcg gtgacgcctt gtcaaagcaa gggtcgttgc cttctgctcc
    11701 gcgtgaagca actggccgag cgttttgccg gcgaagtaat tgaaggctcc caagtcagaa
    11761 aggtcgttgt ccgacggcag aacgagatcg ttctggaacg tttcggtttc cacgaacgtg
    11821 aacagtttgt cgttcggacc ttcgatataa agctgcacct gcgagtgctg gtccgtcacg
    11881 ccaagcgcct ttatgggggt tggacccacg ttcacgacag ttccatcgag tgccttctct
    11941 ttgccaaggc tttccgccca gagctgccga aaccagtcgg cgacatcctt cagggcgtgg
    12001 gaatacggca tcatgactgc cagtcgtttt ggggcacggg gatggttgta catcaggtag
    12061 tgcagtgcgg cacctgcgaa tgccgggcat ttctcggcgg gtacgctggc gtggtactcg
    12121 gccgcgtcag ccgccccggc aagcagttcc tcgatatcga ttccgctcat cgcggcggaa
    12181 agcaatccca ccggtgtgag aacgctgaat ctgcctccaa ctccccgtgg tatgccgaag
    12241 gatacaaggc cctcgcgcga cgcaaacttc cggagatccc ccgcgaccgg gtcggttgtc
    12301 acgatcacat gttcccgcca tcccgggccg attcgcgggt gatcgtgcag cagtttcttg
    12361 acgaccagga atgtgcttgc cgtctctgcg gtgcttcccg atttcgtgat gacattgaaa
    12421 agcgttcggg gcagatccag cgtatcgaac aagccggcga tacgatcggg atcgatattg
    12481 tctgcaacct cgattctcat gccgccccgg cgatccgcgg gcaggaaatt gtggtacggg
    12541 tgccggagcg ccgtgtggca ggcaatattg cccagagctg atccaccgat accgagaacc
    12601 acgaacgtct cgaacttcgg gcgattctgc tccacgcacg cgaggatagc ggaaacatcc
    12661 tgcttcggaa gatcgaggaa cggcagcgta cctgcttctc gtttcaacgc aatctcgcgc
    12721 tgaatcgccc tgagcctgtc gtaaaggctt tccagccggg cacgggctat accaccctcc
    12781 gatcccaccg cttcttcaaa gacattcgcc attgcgaggc gtacccgcat cgagtttttc
    12841 catgccgtat cgttccaggg tctcatcctt gtcctccgtc cgtatcacca ggattccagt
    12901 tttctcgact attccgctca tgcactctcg tatcaatcag gctcatctcg tgcggcgtaa
    12961 ggccttggct tgattcccac acgacgccaa agtgccccga aaacccgtcg gcaagcgcgc
    13021 ggatcaaatc atcagtggtg ccgcgccatc ccagttccca caggcaagtc gtaccgactt
    13081 ccagatttcg gagataccgc tgtcgaacgt cttcttccac gcgcaggtat tcgaggattc
    13141 gtctgtgtgc cgtccctgtc aggatcgaac catgctgcag cacagccgtg gatgaacggt
    13201 actgggcaga gcctacgagt ttgcgcccgt cgacgacgat ttccgagcgc gcgacactcc
    13261 cgaaacatgg cggagcgctt tggccagggg agccgggcga cagatgctgc gtcacgcggg
    13321 ccacttctgc gcgaattcct gcaaggcgta acccacgtgc aagcgcttcg ctggttcgtt
    13381 cgtaggtcgc gtggatgccc ccggtggcca ggggatgttc ccgcgcacaa acgacggaat
    13441 aggtcagctc ttccacgtga aaaaccgcgc ggccaccggt gattctgcga acacaatcat
    13501 acccgtcgcg tcgcaacgcg ttcagttcga tttctttctg cggggactgg gaacgcccca
    13561 ccgaaacggc gtacggcttc cactcgtaaa aacggagaat cgccgcccag ggggcgttcg
    13621 atacaagttg gaagagaacc tcatcgcagg ccatattcca cgctgctggc gcggggggcg
    13681 ttacgatgag acgccaacat tctttgtccg cgttcattat acaccggtgg cggggaacgg
    13741 ttcatccgta ttctggtcgc cgccgtttga agcccttcgc gtcagaagtt catacgcgag
    13801 cctcgccgcc tcttgttgcg cgtacttctt cgttgttccg gttccggtcc cgaggacttt
    13861 ccccccgatg gcaacctcca ccgtgaaagt cttctggtgg tccggcccgg attgtgacct
    13921 cagcatgtat tccggcggcc gtcctcccgc ctgaacgtgc tccagaagga gacttttgta
    13981 attggtgaag atagactccg tctcatattc gcgtagatgg acgaacaggg tggtttccag
    14041 gaagctccgg ctcggttcca gtcctccatc gaggtaaatg gcgcctacaa gagcttcgaa
    14101 ggcgtctccc agaatggagc ggcgctgtct gccacccata ccctcctcaa cgctgctcaa
    14161 caacacatgc tgatcgagat ggatttccag cgcgcaacgc gccagtgcgc ttttcgatac
    14221 gaggaccgac tttctctttg tcagttcccc ctcgggtttg tcggggaatt cccggtaaag
    14281 gaactccgtc gcaagcaatc ccagcacggc atcgccgagg aattcaagtc tttcgttcgc
    14341 ctgtgatacg gggtcgtccg gcgaatcgcc attggaatga acgtaggaac ggtgtcgcag
    14401 ggctgcgatg agaagacttt tgtcgcggaa ggtgtacttg atcgcgtctt cgaggtcgcg
    14461 aattcctccg gccgagccgg tgcgttgcgc tgcagagcct tttcgtgaca gaagccgacc
    14521 tagccagacc atcgaaacac atatacctgt ggagaaccac ggaaaacagg gaacgcgaat
    14581 cagtctttcc aggaaccgaa cacgagactc gcgttgtgtc ccccgaaacc gaatgagttg
    14641 ctcatcgcca cgtccacgcg tttttcccgt ggggagttcg ggacgtaatc aagatcacac
    14701 gcggggtccg gcgtggacag gttgattgtc gggggcaaca caccgtggtg gagcgccagt
    14761 atcgtcgcaa tcgattcgac tgcgccggct gccccgagaa ggtgccccgt cattgatttt
    14821 gtactgctga cggcgagacg atacgcgtgc tctccaaaga cgttcttgat ccctgccgtt
    14881 tcggctgcgt cacccacttc cgtggatgtc ccgtgagcgt tgatgtaccc gacgcgctcc
    14941 ttcggcattt ccgcgtccag aagtgcgttt tccatgcacc gtgccatccc ctcgccgttc
    15001 tcgggcggtg ccgtgatgtg gtacgcgtcc gccgaatacc cgaagccgag cacctccgcg
    15061 tagatcctcg cccctctcgc aatcgcgtgg tccagagttt cgagcaccag tattcccgcg
    15121 ccttctccgc acacgaaacc gtcgcgatcc ttgtcgaacg gccggcttgc gtgctccggg
    15181 tcatcgttcc gcgtggaaag tgcccgcgcc gcgcagaatc cggccatcgc cagttccgtc
    15241 agggaagctt ccgctccgcc ggtcagaacc acgtctgcat gtccgttccg caggacggac
    15301 accgcgtcgc ccagtgcatg cgctgccgat gcgcacgcgg acaccgtgca ataattcgga
    15361 cctttgagcc cgaagatgat acttatcatt ccggcggcca tgtccgaaat cagcatggga
    15421 atcatgaacg ggctcacccg ccctggtcca cgttctttca ggactttgta ctgcgcctcg
    15481 agcgtcgaaa taccgccgat acccgagccg acgatcaccc cgacccgcgt cggatccatc
    15541 ccgtcaaccc gcagatcagc gtcctcgatc gcttcagatg ccgcgtacac cgcgtactga
    15601 atgaaaaggt ccattcgccg cgcttctttt gcgctgatgc gctgcgtcgg gtcgaaccct
    15661 ttcacttccg ccgcgatcgt agtcgagaac ccctcggtag ggaacctcgt aatcgagccc
    15721 gcgccgctct tgccgtggag aagagcctgc cagtactcct ctagtgaatt acctaccggg
    15781 gtaactgcgc ccattcccgt gacgactacg cgtcgggttt tgccgcccac aaagaactcc
    15841 ttgcacgcga agcgagcgag ccaagtcgcc cgcggccgaa ccgactactc cacgttcttg
    15901 cttttcaggt attcgatcgc ggcgcctacc gtgtcgagtt tttccgcctc ttcgtcagga
    15961 atctcgacgc cgaatttgtc ctcgaatgcc atgacgagtt caacggtatc caacgaatct
    16021 gccccgagat cctctatgaa acgagcctcc ggctttactt cttccggtgc aacacccaac
    16081 tgctcaacta ccagatcgcg aactttgtct tcaatcgcca tattttacct cctggtgccc
    16141 tatgcacgtg gtgtggcggg ttgatgccct gacaagcggt actttaaata acactcgttg
    16201 cgatggcacg ctccgtttga acccaccgac ccgttggccg atccgttcac atcgcgatgc
    16261 caccatcaac gttcaagacg tgtcctgtaa tgtacgatgc ttcctctgac gcaaggaaga
    16321 ccgctgcggc cgctacttcc atcggcgtcc cgggccgccc gagtggaata ctggcaagca
    16381 acgtcgctct gactttttcc ggaatcgtat ccgtcatccg ggattctatg aatccggggg
    16441 caatggcgtt cacggtaatg ttacgggaag cgcattcttt ggcgacggtc ttggtaaaac
    16501 caatgatacc cgctttcgca ctggcgtaat tcgcctgtcc cgcattccct acttcgccca
    16561 ctatggaggt aatggaaatg atacgtcccc agcgggcctt cagcatgggc ttcaacgcag
    16621 cttgagtgca cagaaagacg ctcgtgaggt tcgtttcaag cacatcgttc cattgctggt
    16681 cattcatccg gagcagcaga gtatccttcg tgatccctgc attattgacc agcacatcca
    16741 gccgccccca tgtctcgacc acctttgcga cgccggcctc cacgtcggaa cgctgtcgaa
    16801 cgtccatgga aatcggcagg gtttccgcac catgcgcgcg cagtgtttcc agcacacggg
    16861 caagtcccgc tgatcccgtt cggctcgtca tcgccacctt tgcgcccgca agtgcgaatt
    16921 tttcgcacag agcagcaccg atatccccgg acccgcccgt aacaagcaca actcgaccgt
    16981 tcaagtcaaa catcgcgaac tgagtcctgt cattgggtct tcaacagtgc ccgaatttct
    17041 gctgtggttc caactgtggt ggtggtgatc gaaggagcaa tgcgtctcac aaggccctgc
    17101 aaaaccctcc ctggccccac ttcgaagcag cttgcggagc cggacgatgc cagggagcga
    17161 accgtgtccg tccaacgtac ggggctggtt atttgttcaa ccagcagact ccggatttcc
    17221 tcagggtcgg ttgtccgttt cgcggtaacg ttgggaataa caggaattcc cggtgtggag
    17281 atttcctcgc gttcgagggc atcggcgaac actcgtgccg cttcggccat cagcggcgag
    17341 tgaaaggcgc cgctgacttt gagtggaaca atccgcttgg cacccagttc cgaaagcgcg
    17401 gtacccgcgt ttcctactgc ttcttctgct ccggaaatca cgacctggtc gggcgcgttg
    17461 tagttggcaa caactacatc acgtaggccc tggacacttt tctcaaccgt gcgttcgtca
    17521 agtcctacca ctgcggccat cgtcccgggc ttccgggacg ccacatcggc catcgcagca
    17581 gctcgcgccg cgacgaggcg caacgccgcc ccgaactcca gcgcgcccga ggcgcacagt
    17641 gccgcgtatt cgccgaggct gtgccctgcc accacatcgg ggcggatacc atgttgccgc
    17701 agaatctcga acacggccat gctatggaca aacaccgcgg gctgggtata ccgggtgtcg
    17761 ttcagtgttt ccgcagggcc cgcgaagctc attgatgtca ccggccaacc cagaagggca
    17821 tccgcgtcct catacgttcg ccgcgccgcg ggaaactccg cgaaaagatc cgcacccatt
    17881 cccacatatt gggagccttg gccgggaaaa aggaacgcaa tccccatctg ttccgtacct
    17941 acattcgagc cagcgccgcg ccccaggtca ttccggcgcc aaacgcaacc ataagcacga
    18001 tatctccgct ccgcatccgc cccgaggctt tggcctcggc catcgccacg ggaatcgtgg
    18061 ccgcagaggt gtttccgtac tcctgtatgt ttaccacgac cttgtccatt gggatattca
    18121 cgcgtttggc ggtagcctcg attatgcgca tattcgcctg gtgggggatg agcagcgtga
    18181 tatcttcccc ggagaagccc gtctgccgaa gaatctgctc cgccgcatca cccatcgccg
    18241 tcaccgcacg tttgaagaca tccttaccgg acatctggag atagttttcc tcttccttcg
    18301 cgaccggctt cgtcccgccg ccgatacgac agagcatctc cggttgcacg ccgtccgagc
    18361 gtatgtacgt gccaagcacg cctgcgccat ctttgtttcc ggcactgggc gcgagaaggg
    18421 ctgcccctgc cgcatcgcca aaaaggacgc acgtgttcct gtccttccag ttgacgatgc
    18481 gagacatcgt ctccgcgccg atgagtagca cgttccgatt ggccccgcta cagatgaggc
    18541 tttgcgcgag gtggagaccg tacaggaaac ccgaacacgc cgcattgata tccacggcag
    18601 ccgcgttgcg ggcacccaac gcgttctgaa caaggcaggc cgtagccgga aaaccaatgt
    18661 agggcgtcaa cgtcccgagg atgatcatat cgacttcctc aggttcaacg cctgcatcga
    18721 aaagcgcttt tctgcccgct tcagtcgcga gagtcaacgt attggtgtcg tcgtccgcga
    18781 catgccttcg ctctattcca gtcctggtcc gaatccactc atcgctggta tctacgagct
    18841 tctcgatgtc atgattactc aacaccttgg tcggggcggc ggcacccagc cctttgaaaa
    18901 acgctctttc aggatgtcgc atcgatgctg gcttccgaca gtttgttttt gatgtgctcg
    18961 ttcagtttct gcctcgccgc ggtcgcggcc acccgaatgg cattctggat ggcacgcgcc
    19021 gacgaacgac cgtggcagat aatagtaata ccatcgatac ccaaaagagg agcaccgcca
    19081 tattcttcat aattcagtcg agttttctgt ttgttaaact gtctcttgat caagagcccg
    19141 ccaagcatgc tcaacggcga tgattttatc gcatccttca gaacgccggt tatgaattgt
    19201 actgcgcttt ccgcggactt caaaacaaca tttccagtga agccgtcaca cacaactatg
    19261 tccgacgttc cactcagtat gtcgcgccct tccacgttcc ctacgaaatt caggcttgag
    19321 gctgcaagaa gcgaatgggt tttctgaacc agctcgtcac ctttcgacgc ctcctccccg
    19381 atgctgagaa gccccacaga tggattcggc acattgtaga cgaaactgtg atagagactg
    19441 cccattacgg cgaactggta gaggttcagt ggctcacagc tgctgtttgc gcctacgtcg
    19501 agaagggtgc acactccttt cttcgtaggc atcggagacg cgatcgcggg ccgatttacg
    19561 ccgggaatgc ggccgagcaa gagaagggaa agcgccattg cagcgcctgt gtttccagcg
    19621 ctgaccgcgg catccgcctc tcccgtcttc tgcatcttga tggcaaccgc cagcgaggaa
    19681 tccattttgc gacgaaggtt gctgacggat tctgccatcc cgactgtggt cggggcgtgt
    19741 cggatctcga cccctggcac ctctccaaat cgcgccagag atgcctttac cgcctgctcc
    19801 tgcccgacaa gaatgaccct gatcgccccg tcgccctgtt gcgcagcatg aacagctccg
    19861 gcaacgattt catccggggc gaaatctcca cccatggcgt caacggcgat tgtgcacacc
    19921 cgttccatgc caacacctca aattcgagtg tagaacgcgc ctgaacgcgg gcagcgggaa
    19981 atccggacaa agtaaacggc gccaccgggg aggcggcgcc gcgcgtcgca ggcgagctat
    20041 tcctcgcgcg gtgcaacggc gaggcgctgc ccgtaataac cacattccgg acacacacgg
    20101 tgagcaactt tcggggcgcc acaatggctg cacttcacca cattgggcgc ggtgagtttc
    20161 cagtgggtgc ggcgcgaacg cgtgcgagct tttgaagtac gatgttttgg gtttgccaca
    20221 gaatcactcc ttaccgggaa tttggatatc aatccatcgc gcgaagaagc aggtcattcg
    20281 gcctgccttc gacacgagca ggattcctca ttcaaatcgg ccccgcaccg gggacagatt
    20341 cctttgcagt cgtcgtcgca cagaatggtc atcggtacct cgacgatcag actctcgcga
    20401 atatggtcag tcaaatccac ttgggattct tcacgatcaa cagttatcag gacttctccg
    20461 tcgtctccca tttccgccgc ggaaagcccg gcccgcaatt tgacaacaac gtacagcgag
    20521 gattcgatgg ccaggaccgc aggtcttagg catcgatcac aaggacgctc cagttgagcc
    20581 ctcaacgcag cgcggatcgt gactacttcc tgcaggcggt ggattttcgc gcgaacctgg
    20641 atcgccgcga ctatttcggg atagatgaga tccacaccgg ggatttcacc agcccagtca
    20701 acggcgtgaa tcccgtcctg gaacgtagaa aagtcgataa ccatgatatc ccgcttgcgg
    20761 tgaagacaga ctaattataa tacattccaa taccaaggaa gtcaacgcgc gcggcactgt
    20821 cgggactttg tgcacaccgc gggtacctgg ttggggtcgt tgtcacggga aaccgcttgt
    20881 tgcatctttg ttccgtaatt tttcgaaatt cactccacgt ggaaagggcc gttcacatgc
    20941 tcaaagacct tgttcgtgaa tgccgcagta cgcgtcgctt caaacaacac atccccgtaa
    21001 cccgtgacgc cttggtggag atggtggacc tcgcgcgcat ctgcgcctcg ggaagcaata
    21061 agcaggcgct caaatacttc gtgtcggcgg acccggaaca gaacgcccgc atttttccca
    21121 cacttgcgtg ggcaggctac ctcaccgatt ggccgggccc ggcggagggg gagcgccccg
    21181 cagcatacat catcattctc ggcgatacca ccataagcga aggctttggt gttgatcacg
    21241 gaattgccgc ccagacgatc gctctcgcgg cccgggaaaa ggggctcgga acctgtatca
    21301 tgggcgccat ccagaggccg aagctgcaag agatactgaa tatcccttcg cacctccaga
    21361 tacttctggt gctcgccatc ggcgtgcctg cggagaagat ccaactggaa ccggtcggtc
    21421 ccgatagaag catcaaatat taccgtgacg aaaacggcgt ccaccatgtt ccgaagagga
    21481 gcctggagga ggttttggtc aactagcggt cagcggctgg gccgaccccc gcgccttttt
    21541 cccggcgtgg gacgggatgg gcgtttcgac gatctccgca gcgtcttcgt gcgcggtcct
    21601 cgcgttcgat gcgagtgagg cgcttcgaca agcagaaaat cgatccgtcg agatcccaca
    21661 tccactcgaa cgacctggac gctcaccgga tcaccgaaac ggaaggtcat ccgcgacctt
    21721 cttccctcca gcgtaagcga cgtggggtcg aacgcgtacg tgtccgcagg gaggtcgtcg
    21781 accggcacaa atccctcggc gagactgtca ttcaagacca caaacagccc gaacttccga
    21841 acccctgaga ccgtcccgtc gaactcctcc cccacgcgcc ccgcgaggta ttccgcctgc
    21901 ttcgctcgaa tggcttcccg ttccgcggtt tctgcggttc tttccgctgc ggaacagtgg
    21961 tcaccgatgg tgaacaattg ctcgtgcgaa taacagggcg gggttccgtt cagtgcccgt
    22021 ttgagctggc ggtggacgac cagatccggg taccgtctga tcggcgacgt gaagtgcgta
    22081 tatgcatcca tcgcaaggcc gaaatgcccg atgttgtcag gttggtacac cgccttcggc
    22141 aggctccgga taatcaaagc ttcaagcact ggcgccacag ggttcccacg gatggcatcc
    22201 agcaacttct gcaggtcttg caccgaagga gatcggccgt ccggcgcaat cacctgcccg
    22261 agcgacagcg cgagaggagc gagttcctga agtttcgccg ggtcgggacc tgcatgaaca
    22321 cggtacaaca gggggtaatg tatttgtgaa agatggtcgg cgataacgct gttcgcgagc
    22381 aacatcagct cttcgatcac acgatggctg tccagacgtt cccgccgacg aagcgcaaca
    22441 acccttccct gtgcgtcgac ttctacgcgc acctccggga catcgaagtc cagcgctccg
    22501 tgacgcgatc gcttccgcgc cagttcaccc gtgatgtcgg caagccgttg cagcattccc
    22561 gcgtgcggga acgcagggtt gtccggccgt gctgacccgc ccaacgcctc ttgcgcttct
    22621 tcataagaga tttttgcaca ggagcgaatc acgctctcga ggatctccac ccgaaccggc
    22681 agaacgtttt cgtccagcgt gatcagaacg ctgactgcga ggcgatcctg acgcggcaac
    22741 aggctgcaca ggcccgcagc aagctgcggc ggcagcatat gtaccacctt gccaggcaga
    22801 tagacgcttg tcccgcgcag atacgcttcc ttatcgatag cacccttttc aggcacgaaa
    22861 tgggaaacgt cggcgatgtg aattcctacg acatcccgcc cggcgtcgtc tttcgaaagc
    22921 gagatcgcgt cgtcgatgtc tgcggcatcc gaaggatcaa tcgttaccgt ttccagccca
    22981 cgcaaatcgg ctcttttccc tgcctcctcc gcagctcgtt ccgcaacctg ttgagcttca
    23041 cgaacggctt cggccggaaa cgaaagccgt attccgtggg cgtgcaatat ggcggtgaca
    23101 tccacccacg ggtcgccctg tttcccgagc gctgttccga acctcgcagc gggcgcagac
    23161 gactccaggg acaacggttc cagaactgcc gttatcacgt ccccttccga cggcgctttt
    23221 tcctcccctt ccgcaccgag gaaaacgagt tgcaggtctg gcaattgagc cgaaaatggg
    23281 cgaatccacg cgaaaccaac gcctttgtga aacgtcccca cgatcatgcg ttcgggtcga
    23341 taagcaatcc caagcaccac cccccgaccg agattgcgcc ttccgcgcgg gctctgttcg
    23401 acaaccaaca cggtgtcacc gtccagggcg tttcctatgt cttccgccgg tatgtagaga
    23461 tcgtcctccc gaccttcgac ggcaacgaac ccataccccc gttccgcaag acgcaaggtg
    23521 ccaagtcgcg cgccaaggcc cgacgcgctg gcgtatcgct ggcccctccc ctcgtaaatc
    23581 ttaccttccc ggcgcaaatc catcactgcc tgccgaaaca atgggtcgtc ctgcgtcgag
    23641 tgcactgcgg aagccagttg ctgcaacgac aagggcttgg cccgcgctga aagggctgcc
    23701 aggatggcct cacccggtgg aaggtgtgct cgcctcgcag gctttcgcga accggttcgc
    23761 atcagcccct attcctgcgc ggcacggatg gcattctcga aaatggcgag cccgtccggg
    23821 cggctgcggc tggcgttacg cgtccattga ggatggtgag taggatgaat gtggcgctcc
    23881 ggatgcggca tcattcccat cacgcgtccg gagacgttgc agatggcagc gatagaccgg
    23941 tcggcaccat tcgggtttcc gggatacccc acatcgttcc cgagggcatc cgtgtaccga
    24001 aacacaacct gatcactcgt ttcgagacgg tcaagcacct ccagggatga cgcggtgaaa
    24061 cgcccctccg catgcgcaac cgggaggtaa atcggctttt ctatgccgcg cgtgtacacg
    24121 catcgcgagc tacccggttt cagccacacc cagcgatcct caaactttcc ggaaagattg
    24181 cccgcgagcg tacaggacgg agtttgttcg ttccatcccg gcaataggcc ggtcttaacg
    24241 agtacctgga aaccgttgca gatcccgaga acgaggccac cacgctcaac aaacctcaga
    24301 agcggctctg agagccgtgt catcagttca ttcgcgagaa ccttgccggc gctgacatcg
    24361 tcaccgtagg tgaaaccacc gggaatgaca aggattgacg tctcctccag caacgaaggc
    24421 ttttcggcaa cacggttgac gtgtatccgc tcagcctcgc ctccggcaac ggaaaacgca
    24481 tgagcggtct ccacatcgca gtttgagccc gccgcccgca gaaccagtac atgaggacgc
    24541 atcgattccc ctgcaaaaaa gtttacgact tcctgccaac cgctcctgga attcctcttg
    24601 cgggcttcct tcgttgaaca aacaagatga ccacgcccgc gatgacaagt gcaacgctaa
    24661 gaacttgcgc aagactcaac ccggcatacg cgaaatgcgc tttcctggca atttcgaagg
    24721 caacgcgttg cgattccgcc agcgaagttc caccgtgtgc atacgcgtac atcgcgtata
    24781 ccccggaatc gaaccgccag aactcggtaa tgagacgctc cacccccgct acgaggaagg
    24841 tcgctgccac catcatcccc ggagccagtg tcttgtggcg caacacgaac cagaggtacc
    24901 cgaagcccac aaggcaaagt ccgatctcat acaacggcgt cggatggacc atcagcaccg
    24961 tcggcacggt cccgttcgga aaactcattc cccagggcag attcgttggc ggtccatagt
    25021 ccccgtcgcc tgaaagaaaa caacccgcac ggccgaatgc gtatccgagc accagtatgg
    25081 gtcccagcaa atccgccaca tggaggaatg acaccttcgc ccttcgcacc acgaaccaga
    25141 tgaaaagaac cgcacccgcc agcccgccgt aaaacgtaag acccgcgccg gaaaacaggg
    25201 tattgagagg ctccgcggcg aattcatccg gcgcttccag cgcgatgaaa tacagcttcg
    25261 caccaatgat tccgccgaac atcgccgcca acgccaccct gtcagccaga tcctccggta
    25321 gattccatct ccgcagttcg cgccggaata gccacgcccc gacaatgaac gcacatgcaa
    25381 gcatcgcgcc gtagctgtat accgtgacgg gtccaatttt gaacagctct ggatacatcg
    25441 cacttcctca catgtccgtg gacacaccac cagcggacta tcaaatatac gatgcatcct
    25501 gaagccggtc tgcgccccct ccttcggagt tgtacacccc ggccgccggt ttccggccca
    25561 ggcgccggaa cgcgaagctt gacccactct ccccgtgatg tcatcttttt acaatgtctt
    25621 ttgcgccgac ctgcggctgc ttttcatccc ggcgttccgc cggttcccgt tcgtttcccg
    25681 ctggaggatt tctcttatgc cccttctcac catgaagcag attctggatg acgctatcgc
    25741 caagaactac ggtgtcggcg ccttcaacat caacaacatg gaacagaccc tcgccatcat
    25801 gagcgccgcc gacaggactg agtcgccggt tatccttcag gccagccgcg gagcacgcaa
    25861 atacgccggt ccggtaatga tttacgggct cgttcaggcc gcgatgaagc tttacccgaa
    25921 agtccccgtc gcgctccatc ttgatcacgg caacaacttc gagacgtgca aacaatccat
    25981 ggacgatggc tggacatcag tgatgatgga cggatcgctc atggaggacg ggaagacgcc
    26041 gtccacattc gagtacaatg tcgacgtgac gtcccgggtg gtagccgaag gacacaagcg
    26101 tgggatcacc gtagaagggg aaatcggcac ccttggcggt atcgaagacg ggcacggagc
    26161 tgacagggta aagctggctg atccggacca ggcggtggag ttcgcccaca aaacaggtgt
    26221 agacgccctg gctctcgcgt tcggaaccag ccacggcgcg ttcaaattca aggcaaaacc
    26281 ggtgctggct tacgacatcg ttcaggcagt caacaagaag atgcccggtc tcgcgatcgt
    26341 cagccacggt tcttcgagcg ttccccccga gctcgttgcg gaaatcaaca agtacggcgg
    26401 caaaatgccc ggcgcgatgg gtgtcccgtt ggaggacatc gtccgctcaa tcgatttcgg
    26461 tgtccgaaag atcaacatcg acaccgacct tcgtctcgcg gccactgcta ccatccgcaa
    26521 agtcttcgtt gaaacgccgg agaagttcga catccgtgat tacctcaggc cggcgatgga
    26581 agccatgtcg cacgtcgtcg aaacaaggat gatcgccttc cgcaccgcgg gaaaggccag
    26641 caatttcacc aaagtgatct ccctggagga gatggcgaag gtgtacgcga agaagtagtc
    26701 gtcaagacca caagacgtcg aaatgcaaag gggcaggcct tatcaaggcc tgcccctttt
    26761 ttccgctcga aaagtgtcca gcgttcctca gttccacgca ttccggtaga tcagatcgac
    26821 cagcagcatc gtcttgtggg cgtcaccgaa actcgtttct ggttctcgat ccgctttcag
    26881 gctatcgata aagtgccgat tctccgcggc aaaaccatag tacacccggt tctcctcgct
    26941 gccggccact tcctttgatg aaaacaccac cggctgaccg ttgtccgcca gaatctccac
    27001 tgcagcgtca gggttgcagt acgcgcttat tccgtccgcg tgcatttcga acacatgcct
    27061 gcgcccgccc acgttccagt ttccaagcag tgccccgaca cagccgtttt caaaacgcac
    27121 cagcgcgttg aacatgttcg cgtaacttgc gttccttgac tgcacgtcac tcacaagatc
    27181 cactaattcg gcgcccgcca tccaccgcaa catgtcgacg gcatggatca tgtcgcttgt
    27241 caggatgtcc actgcacccc ggtagtacgg gggttgcccc cggtagtcct tgtagaacgt
    27301 cgatacgacc tggatcacat cgcccttcgc cgtaaccatc tccctggcct tctgcatgac
    27361 agggatgaac cggcggttga atgcgaccat ggtatggact cctctgttct tcgccgcggt
    27421 cgccatctga aggcactgat cggaggttac tgctggcggc ttttcgatga acacgtgctt
    27481 gccccgctcg atcgcatcca tcaccggttc aaatagctgg tgcggtggca tcagaatgta
    27541 gacggcgtcc acctgttcat tgtccagcat ggaacggtaa ttcgtatagc gcgccgcgat
    27601 gcggaagcgg tccgcggttt tgcgcagccg ctcctcgtcc aggtcacaca acgctgtcat
    27661 ctccacgtct ggcatttccg caaggctcgg ataatgaacc gcgtttgcca ttccgcccgc
    27721 tccaatgagg cccagccgaa cccgttccat cacgtagact cctcaaagaa aaaaggagaa
    27781 ggtgcgcgtc tgcgcgagcc cttctccggt ttgcccatca caagcggatt ctcattcact
    27841 tttccaacag gaatttctcc gccgctgcga caagggcgcg gcgcgcttcc tcgatgggga
    27901 tcttcatttt gcagcctgcg gccttcttgt gtcctccgcc gccaaactga gaagcgaact
    27961 tgtttacgtc ggcatcgccg gctgaccgaa tgctgacacg aacggttccg tcggctcctg
    28021 gccgaaggaa catcccgatt tcgacccctc tgatggtcac cggccagttc ggcagatcct
    28081 ccacctcaat ggcctgcccc tgagcattca ccggttcctc gtagatcagc atggaacaga
    28141 ctttgccgct cgcggacaac tccattgtgg aaagcgcctg tccggtaacg cgcacgctgt
    28201 cgaacgagtt cttgtagtac agctccgttg caacctggtt gacgctggcc cctgcgtcta
    28261 tcatgtccgc cacgatacga agggcctcag gagtcgtgtt cgcgaaactg aagcgccctg
    28321 tatccgacat gattccgcac acgatcatcg tccccagagt ggaatctgcc ggcaccttga
    28381 gggccttcag gaggcgcgca acaatcactg cggcggcgga cgctgtcggg tcaacatacg
    28441 cgtactcccc gagagggtca cttctctcgt ggtgatcgag atagatcgtg accacgtctt
    28501 tccacgccga tttcccgatt gtgccggcct cttccgcggg aaccaccggt ttctgaaaac
    28561 gccgccggat gtccccgcag ttgatgttct cccgaacggg ggtatcgata atcacaactg
    28621 cgtcaggcgc gtagtccgca gggaccgccg tcagtatcgt ttcgatttca ggcagaaatc
    28681 gaaattgaat cggcgtctct tcatcattga caactatcgc ttccttgcct gccatgcgca
    28741 ggagtcgcgc cattgcaagc tgcgaggctc ctgcatcgcc gtcgaaatgc atatgggcag
    28801 aaactacgaa gcgattcttc gaacggatta cccgagagat cgcattgaga tctgtcgcca
    28861 tttactcctc ccgccttcgt gtaagcccgc gcattgtccg ggttttcctg cacgcaacac
    28921 cgaactagcc gctgccttgc ccgatcttgt ccatcgcgca agcataccgg ccgcgcggcc
    28981 agaaaccaac gaggaacaca cacaaagatg gctcagagcg gctattgttg caagagccgt
    29041 tcattccccg gttcgaactc gagattgatt gggaccgcgc ttcttttccg tcggcgaaag
    29101 cgctccacgt gcgcaaaacc cgtttccctc gccatgcgca gaccaaaaag gaccccggct
    29161 cctgtatgct ccaccgaatg ggaatccgaa cccacggtga tgcgctcgcc gccgagttct
    29221 ctgtaccttc gcaacagcgc ggcagacggg taacactcgg ccgagggttg ccgaagtccg
    29281 gacgtattga tctcaagcgc aatccgcttc gtgaccagcg ttgacagaat ctcgcccagc
    29341 gcctcggcgt gtcgtgtttc gtcgaatggc ccgaaaacct gagtgccgta ccgtttcacc
    29401 acgtcaatgt gcccgagtgt atcccagatt ccaaagcgcg cacaacggag taccacgtcc
    29461 cagtattcac cgtacgcctc acgttctgtc tttccacgga aaaagtcgag cgctcgcggt
    29521 tctgagatgt tgccctttcc ccgaaggtag tgcaccgagc cgatcacgaa atcaaactct
    29581 ctctcctcaa cccatttcgc tgtttcgttc tcaaaatcgg ggcaatagtc cacctcgatc
    29641 cccgccacga ttgccagttc gtcacggaat tgttcacgac atctgcgaat agactccatg
    29701 tatgcatcgt atcgaaagta tcccgtgttt ttctcttccg ggataaaatc catgtgctcg
    29761 gtgaacgcga tctcggatac gccgcgttcc accgccaccc ggcagatatc tgccatggaa
    29821 gccgtgcagt cgactgaaaa ttcggaatga tgatggtaat cgaacacgtg cgcctcattc
    29881 aatctcgctg aagcaggttg gtctcagggt tggtcaaatg cgccagaatt cgaggatctc
    29941 ctctgcgcac cgccgccagt ctttgtcggt ggcgtcgatc gttctgtgtt ccatgcttgt
    30001 ccaattcaag ccgctgacaa aggcacgttg aacacccaca taatgcgtgc cgaggtcgcc
    30061 cactttttcc tcaagacgtt tctggtaggc ctgccatgcc tgcccccgct gtcgaaacgc
    30121 ttccgctatt cgcgaggaaa ccatggattc ctcaaccatc agaacgacga cgcggcaatt
    30181 cagcctcgcg agcgtgagat cgatatcccg gtacgtctca aaaaggccgt cagcatagga
    30241 aatggcgttg gtgaaatgga accgttccag gaggtaacac agctcgcgtc cgctttcttc
    30301 cgaaagcaac ggacttgcca tttcgatctg ccgcgccgca tcgaggatcc gaagattcct
    30361 gtgcatcagc gcgcggtatg catctgcatc ccggttgcga agatgttcgt tcgcacgttc
    30421 ggtaaagaac tcgctcaaaa ggagcttcga tgaccgtctc tgccatgcgg gcaacgcctg
    30481 gattgctctg atcacgctcg tcttgccaga cgcaacaatg ccttcgagca gcagtcctgt
    30541 cacgggttga ctcctcgaat tccccg
//
LOCUS       DAOSOO010000012        28258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1679_length_28258_cov_5.571074, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000012 DAOSOO010000000
VERSION     DAOSOO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 28258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28258
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1679_length_28258_cov_5.571074"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PLT86_01835"
     CDS             complement(<1..1650)
                     /locus_tag="PLT86_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35387.1"
                     /translation="MSADRRLRVLMPVLLCLVSAAPILLAPGTSRAAPGSVTNLTATA
                     TGTNEITLSWTPYSGNDFARYEIRRGTAAGVTTASPLVDTLSAKTASSFADRTVLPYT
                     TYYYKVFVIDTAGVAGSSAEPSARTLALSYPFADTVAAANVNFVPTGTWAIVANSPGE
                     GDAYSGSWHWSDSPGGNYAPNSSGTLTLAIRLGTATMPALTYRERYAFETNVDYGHVE
                     VSADGTNWYAVASVTGSQTTWAERRIDLTQWAGYDGIRIRFRIAASSSVESDGWHLDS
                     FSINETPLTSMPYPLREDFESGTSRWLLGGWSPATEGTNGPTSIHDSKTGNYPALAYN
                     GMVSTGVFDLTFAKNPTLVFWHRYNIFNDHSYGHNEDDWGRVYVSSDYGRPGSWVNIA
                     SYNGSQPTWTRVQLDLSQFAGSPSVRVMFLLDDNVDAANASFNRQADGWYLDDIRLED
                     LPRAVEINPIVSSSMHQVDLTWSPNTEGDFARYEIYRATSTAVTRSSTLVSTITNAAV
                     SSWTDSVAMIEPTQYSYRIYVVDTLETLSLGSNVVTATYTVP"
     gene            2128..8484
                     /gene="uvrA"
                     /locus_tag="PLT86_01840"
     CDS             2128..8484
                     /gene="uvrA"
                     /locus_tag="PLT86_01840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT35388.1"
                     /translation="MSADQIVIRGARVHNLRSVDLTLPKNRLICFTGVSGSGKSSMAF
                     DTIYAEGQRRYIESLSAYARQFMEQMEKPDVDQISGLPPAISIEQKAAGGNPRSTVGT
                     MTEVYDYLRVLYARLGTLHCVQCGKPITGQSRDRLLDQILQHPPGTRLNILAPMARGR
                     QGEFLDLFEELQRQGFVRARVDGRIVNLSEDPSLDRHAKHNVDVVVDRLVMREGIRSR
                     LAEAVDTALNVGNGTLIVNLVRDSEDPGTDIIMGTAYACAECGISYEQPTPQLFSFNI
                     PTGMCPTCLGLGTQVKINRDLLVTDPSKSLREGALATVDVDRNRWLRHLYHGVLHHFG
                     DCGLDTPWKDIPELAKEKLLYGLDGQRIRFRYARNPGERGWTHLDTFEGIIPMLERKF
                     RESKDEGVKEELGAFTSAVNCPDCGGMRLRPEARAVKLGGLSLPELCAMPIEEAHGFL
                     ATLPLSSTERQIGQDALNEILARLEFLMNVGLRYLSIDRTAPTLSGGEAQRIRLASQI
                     GSGLVGVLYVLDEPSIGLHHRDNHRLLNALGRLRDLGNTVIVVEHDEDTMRAADIVVD
                     FGPGPGVRGGAIVGIGSAEDLTDIPDSITGDYLAGRRRIEIPETRRRPNGNWLQITGA
                     RHNNLKNVDARIPLGVFVCVTGVSGSGKSSLITDTLLPALMRHFFHARLPVGPHDAIT
                     GIEHLDKVIHIDQSPIGRTPRSNPATYTGALDPIRRLFAELPEAKVRGYTPGRFSFNV
                     KSGRCDACEGNGATRVQMDFLADVWVPCPACNGARFNAETLQIRFKGKNIADVLDMDV
                     QDALRFFENIPSVHRVLQTLHDVGLDYMKLGQPSPTLSGGEAQRIKLARELCRRSTGR
                     TLYILDEPTTGLHFHDVSHLLEVLHRFCDEGNTVVVIEHNMDVVKTADWILDLGPEGG
                     DEGGRLIGTGTPEEVAEIDGSWTGQVLRDVLHGKVYRRATSRVSEDDGHSRAAIAFDR
                     DEYRPMERITDITVVGAREHNLKNVSLAVPREKLVVFSGVSGSGKTSMALDTIYAEGQ
                     RRYVESLSAYARQFLGDIQKPKVDQVEGLSPAIAIEQKGAGRNPRSTVGTITEIYDYL
                     RVIYAQIGQVYCPDCQIPAGAQSSSEVVERLTEEFRGRRIMLLAPLETEKGGDYDDAL
                     ARLRKEGYLRCRIDGVVHRLDEGIELDRRRNHSLEAVIDRLEISDDERGRLGEAVETA
                     FNKGEGRLVVAPLDGGDDIAISRHISCPSCGKSFEKLTPQMFAFNRAHDISASGMCPA
                     CQGLGTQQGLAEAAVIRSEKLSVAEGAVDLWGKPKGMFRALLEAAGNALGFDLHTPLR
                     EMTQAARTALFFGDESRWISCTERGIEFQYLGVFPAVDRTLRLTPQLRDRLGQVLSDV
                     PCSTCGGTRLIPQSRAARLRGVPIAELCSWPLAQTRAFFDQLSLSPREQQVVGEVLTE
                     IRSRLRFLEEVGLGYISLDRRAPTLSGGEAQRIRLASQIGSGLTGVLYVLDEPTIGLH
                     PRDNALLLSALEHLRDLNNTVIVVEHDSDTLNKADYVVDFGPGAGNSGGEVVATGTPG
                     EIRQNQASLTGAFLSGRRTIPVPRQRRAGTGKAIEIIGAREHNLKDIHAVFPLGTMTV
                     VTGVSGSGKSTLVNDTLHRALACALHKAQFIPGAHDEVRGTSFVDKVILIDQEPIGSS
                     SLSNPATYSGVFDVLRELFAQVPEAKMRGYTARRFSTNVPGGRCERCWGYGKRHIEMH
                     FLPDVWVECEDCHGLRYNRETLEITFGGVSIGEVLQMPVEVALEHFQRVPRVRRLLQT
                     LCDVGLGYMELGQSATTLSGGEAQRLKLASELARPSTGRTVYILDEPTTGLHFADTER
                     LLDVLNRLVDQGNTVIMIEHNVLVIQAADWIIDLGPEGGDAGGYIVVAGTPEDVAACE
                     RSHTGAVLRRALAQKVPAVSNRKDAREAIAVVETETRGLEEFPEEDVPSGGPAPPWKT
                     DGIAWHTSDNRLREGRQPLWKGALIFWIIDQIKKIAPVSVVWSNQHLVSVYPERARRW
                     IAFRTDSWQFVTMEVRVPKGSFDGDKLRKQLDLKPFDELDDVPLYGSWSRVTMNGRIR
                     TWTMVEIVAFRQEELDTEGFRSFLRDAYQLFMDHEAKGGKARSG"
     gene            8491..9624
                     /locus_tag="PLT86_01845"
     CDS             8491..9624
                     /locus_tag="PLT86_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35389.1"
                     /translation="MVRDLARVGMDSRERLLTAINNGKPDRLPVQVHRWMPYYLRTYL
                     GGIDQWAAYEQFGMDMVIYSDPVVSVDDTEQSRWIRERVLCETDREGVRHWKIRVRTP
                     GGDLTGAEAGNDITTWTTEYLIKTERDFELFEKYCPSPTFDWSPVMRDLNRLGNRGIV
                     RTASRGYGQGAPWQDLCTLMGTVSAIMTALDEPERTRHMLDVILAKRISTIEKSPPNP
                     SDIVSTGGGAGSDTVISPDLHARFCLPYDRAYHAALREKSPRTKIVYHLCGGVMNLLD
                     LVATNGADGLETMTPPSMGGNADLAAARRMIGDRLFFVGGFDQQQGFERGTPEDARRL
                     VVQCHAACPDGGYICCPSDHFFHGNPENIRAFADAARECRYDG"
     gene            9968..11146
                     /locus_tag="PLT86_01850"
     CDS             9968..11146
                     /locus_tag="PLT86_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35390.1"
                     /translation="MRRGLLVLVAGIAVCAAVCASPVHAVNRYPVSIGISYGSWTPSM
                     DAYNVRWVDQSNPTMINVGGTLTPVYIRQPLDSAQTIWGKRVGPESFLFSSSSGLGLN
                     AKLRLHNDLFALVEYDSWKQSVGSRRNYGGMIGYEANEVKLNPISVSLLYYIPTEAGK
                     WWPHIYLGAGGGSVLVERSNSQLTNTGILSTVTSTGSGPLFVGMAGLEYTVPALQDRL
                     TLFFQGRYTMGSFDDQFPVLGSTGSQLIDPVTGKPRKEDASVSIQGSQMKFGLSVNFG
                     QLVSRPTKGVLSGFLEANRRRGGYAMAPSRGMQSGGYAMMVPYSSESVQVVEGAGQVD
                     EDRIRQVIREELLGARIGVTSARPVDDLAEQQLRSIRERRLQAEQELQQLQELLREEG
                     "
     gene            11174..12508
                     /locus_tag="PLT86_01855"
     CDS             11174..12508
                     /locus_tag="PLT86_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOT35391.1"
                     /translation="MRWVTLLFSAGLLFGAWVPGGASEHCCSGALDRLHGAGYENLAC
                     ETTDSSVVVWCENRRIRYPVVWMIEALTVVASSVPDDVVVNVVAERLAVPVTSLSARA
                     GDVRAWVKGRIGTEEFRQLLRVEFDGSECPSERRNSSLRKVDLAIGPGRILSEFGLPG
                     VWIRANFDVSTEFSTAFLPGLNASGRMFFPAYNTGGPVYNDSRYNELRPGSALLSYLR
                     PVGKSGFLSVCGGMVEMANWRYDSYGIVVDAKQYSPDGTWGFGGSFGYIAPGRYQVLN
                     DANQRYRTWVFVWPLYNTPYEAWTSYRVTGFDLRITARWGRYLVGDRAWRIDVERSFG
                     ETKLTVFGIQSNGYSDYLKRVDRQNTRLLGGVRMEIPLPPRERGMPERFRVTSTSAFA
                     WSYRYRVGDVGVDLVTKQSVEDHVGAYNPVAVLNNLERARNRVRARQSEACQ"
     gene            12538..13839
                     /locus_tag="PLT86_01860"
     CDS             12538..13839
                     /locus_tag="PLT86_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35392.1"
                     /translation="MKQKNRSLVVLLGLALTAIGGGCSRSVVSRTQESNWVTPPPATS
                     ISVTLNAPTGGGAGDIQGTRVVLISGGSILRDTTLASVTTAPFKFAFGNLAGVGTYQV
                     AIVKTGYTLQVTTASITGSAVPQVVFTLRKLENSNTISVASSLVTSAGTTVTIPQPPV
                     VTGTYSTAAPAQVTLPANSGISSVSVALQTASEVPTISTTQSVVSAVQIQTDATTTGT
                     VTVKFPLPLNSSDFAAAAGGTVDVLRLNLTTMTWVKIGVATIGADGTATYNVPGLTNT
                     NTVLCIGKTPSLSTAVQTVSTSTLLTPDQVANLVMSGVTTTTVPLNVSLGLGKASRSG
                     EVGGAAIDPPAGMDPAYWQMVLGVLMARLPELENYFKNGTQQVDYPLPKEQATLQKDR
                     RSYTFNFTYTWNTGVSKTFRIIVTYDHINIKPITHTSGVGG"
     gene            13948..14439
                     /locus_tag="PLT86_01865"
     CDS             13948..14439
                     /locus_tag="PLT86_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT35393.1"
                     /translation="MASVNKVILIGNLGKDPVVDQTPSGTPRCRFSLATSENFTDREG
                     KKQERTEWHNILIWGKLAEVAGQYLRKGRPVYLEGRIAYRSYEDQEGNKKNFTEIVVT
                     SMQLLGSRSDQSGYDSDVPPPEEPLSSTSSTPRRNAPPRKQKETGIELPEDMPAEDED
                     LPF"
     gene            complement(14470..14949)
                     /locus_tag="PLT86_01870"
     CDS             complement(14470..14949)
                     /locus_tag="PLT86_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOT35394.1"
                     /translation="MTRDDVIGFIAQRILRINGHVPWRVHFTSRVVNPERVVRGKNTF
                     PGDSPGCYIQAANGIYIGDNTNIGPGVGLISANHDPTDNTRHLPGPPIRIGRDCWIGM
                     NAVILPGVTLGDGVVVGAGAVVTHDFPDRVVIAGNPARVIREIRDGTNNNEESVPSA"
     gene            15117..15707
                     /gene="hisB"
                     /locus_tag="PLT86_01875"
     CDS             15117..15707
                     /gene="hisB"
                     /locus_tag="PLT86_01875"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT35395.1"
                     /translation="MARSAEVHRSTKETNVRIVLSLEGNGVFHGSTSIGFFDHMLTLF
                     ARHGGMSLSLDCKGDTQVDGHHTVEDVGIVLGTAIAQALGEKRGIRRYAAAFVPMDES
                     LARVVVDLSGRPYCVIGAELTPAKIGDFDVELAWEFFRALSVHAKCNLHIDLLRAGNA
                     HHAVEACFKAAARAFSEAWTVVGSESGEIPSTKGIL"
     gene            15707..16759
                     /locus_tag="PLT86_01880"
     CDS             15707..16759
                     /locus_tag="PLT86_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT35396.1"
                     /translation="MADRPETVRISLVIVTYNRADELIRLLQSLQNDLARPDVELILV
                     DDGSTDGTLERVSHLLAPISDRATVLRQPNSGPGIGRNRGILRASGEIVVFVDTDCVV
                     HPGWLSALIEPFSDPKVGAVGGPDRSQPGDPLLTRLIDYLMTSFLTTGGIRGAKKTRG
                     GSYHPRSFNMAARRDAAISAEGFPTIWYGEDILFSRRIERAGWRLAFSPEAWVYHRRR
                     TTVRAWARQLFRMGRARWWMGRYDRGLLEPLYVVPLLECSALALAIGVVAITGNIGLL
                     TGSFFGVVAAYALALGADAARRTRHPAAFFAAPLLFALREASYAAGSIAGIFSKIPDL
                     TGALPRMAAEKRETDT"
     gene            16756..18768
                     /locus_tag="PLT86_01885"
     CDS             16756..18768
                     /locus_tag="PLT86_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35397.1"
                     /translation="MKPRYHLRFQIAPGPNLVADSQILADFCNQHNVEEVVLFVAAEE
                     LNNGLLSETEETQWFDTIRTAKQVLKNAGIVVSLNPWMTVLHTDRGRHFPPDRTFAPM
                     VSPEGEVSTACASFADPAWRDYIKHLYGRFASLGFRVLWVEDDFRFHNHAPLTWGGGF
                     EQPVIERFAARIGKPVTREEIVKAILAPGKPHPWRAIWMETWREIHLETARIIANAVA
                     DSSPVQSTVGLMSSHPSSHSTEGRDWKALFEAFTVSGRVAHRPHFAPYSEATGKTRDW
                     SFMMLDVQRNFRPPQSEVAPEIENFPFTRWSKSDAQTWSEMALCQFFGSDALLLDLFP
                     FTGNSANREPEIGELLDRSRPALEWIASRFSKDMQTEGIGTVWKEDTHAHVRTTRGTS
                     MSELNADPFVAGNFLLPYGVAASMRRQPVTALMGNAVWALSDDEIAGALAGGTLVDGD
                     ALDILTRRGFAELLGVLFSGWEDRDHSLYSIEEVVSPRAGTDVGLVFNTNVVPRIARV
                     EAMPGAEEWTTIITPARQRVGAGIVAFENRLGGRAITFATPGPACLPRSFQRQRIVHQ
                     AARFVSRDTPLTMVTGGAYLMPLHLKNGGIHRVAVYNGSPDPARPVVWDSRTRTRPPR
                     VTLLAPLRDPMAGDVTPLHAPSGETGYECTSSLPYHGYAVFEWDDA"
     gene            18783..19832
                     /locus_tag="PLT86_01890"
     CDS             18783..19832
                     /locus_tag="PLT86_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT35398.1"
                     /translation="MNEGLLLSDLLTHAGRPVASRNDWNAKAEDIRARILNVLGAFPA
                     ETPALEPEVLEEVREDGYVRRLVRYASEPDERVPAYVLLPEGQKGPAPAALCLHQTIA
                     EGKRETVGLLQDTHKAYALHLVRRGYVCIAPDHLAAGERITPGLDAYDTSAFYARHPH
                     WSAVGKAIWDARRAVDYLTTLPEVDPARIGVIGHSLGGHGAVFAAAFDERLKACVSNC
                     GVSTFSGSPNPLAWARDRWYVYFPALRPIFLENRRPPFDMHEFAALIAPRAFLTISSL
                     TDESMPVNPYGLQEFAAQLSRLYQFVGAGGKFAWFYHDRGHSYPPESRALAYEWLDEM
                     LGWHSTEAYDGRPRK"
     gene            complement(19932..20951)
                     /locus_tag="PLT86_01895"
     CDS             complement(19932..20951)
                     /locus_tag="PLT86_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35399.1"
                     /translation="MKTRFVPVCLFASLLALGPATALAQSYQSVANDISDLILIHRYP
                     EAQARLNALQRQYPAATDLQFQELVLLFTWIDDYGIADSLNRAFRNQVDAVVTSANRD
                     LARNPNDARALFYKGSALAYRALYRSYVEGIGRNIGSLLTDASSGMAELEKARAADPT
                     FADPLIAIGKYKHWKSKKLPWPFATAQEGRDGIRMLEQAVRMGVLSDGGAEQTLAWIY
                     MSERRYDDAIALVQPYVAKYPTSRFFLEIVARAYQEKGDYVRAGALFGRIMNGLSPDE
                     RARPFVVMKYERWIAKLRLQQGRRDEACEIARRLRSLNYAGVHRDWLDRKMEEVHRIE
                     REACR"
     gene            21274..22164
                     /locus_tag="PLT86_01900"
     CDS             21274..22164
                     /locus_tag="PLT86_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOT35400.1"
                     /translation="MRLITRGDLDGLTSAVLITSMEKIDSIELVHPKDMQDGKVEVTG
                     NDIVVNLPFHPKAAMWFDHHFGYDAQEVPAKFKGKYGVSPSAARLVFEYYNHPSLDKF
                     GELVGETDRVDAAQLTLDDILHPKRYILLSYTLDPRTGLGGAFRSYFMHLLDWLKVHP
                     IGEVLKMPEVKEKVDTILKNEKDFQKALKKHSKLDGNIVVTDFRRLAERPVGNRFVVY
                     ALYPEANVWMNLFKGRTADITVISLGHSILNRTCNTNIGQLLSTFGGGGHRGAGTCQV
                     PSKSAGRVIKKIKEILKANG"
     gene            22241..22849
                     /locus_tag="PLT86_01905"
     CDS             22241..22849
                     /locus_tag="PLT86_01905"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016109307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOT35401.1"
                     /translation="MQAFPRRLYEADTAEAARRLLGCRLVREVDGERMAGIIVETEAY
                     LGTEDLGCHSARGRTNRTEVMFGPPGHAYVYLIYGMYHCLNVVTRPEGDPQAVLIRAI
                     EPVEGLEQMRRNRSRRQRKSSPVDRELANGPGKLCEALGIDGSFNGVDLAGKALRIEL
                     MPPVPNQDIAAGPRIGIAYAGEWAEKPLRFWIRGNPCVSTKG"
     gene            22877..24028
                     /locus_tag="PLT86_01910"
     CDS             22877..24028
                     /locus_tag="PLT86_01910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT35402.1"
                     /translation="MRVTYQLPNCRVSADQPVLVGGQEGHFWFPHLFRLRGNRILCEV
                     TVTADEAQGDWPAVLFLSEDKGAHWRRVADLETAGPAATRLGPGTMLLMPYENWPSAP
                     GDRRNTRANGTILRDSAQGGLDVEPAEVTFHGFPRDLADYHKGEVALFTNGNLLPTRD
                     GGLLSTVYATFAGETKYDLLAMVSYDRGFSWQFRSVAAVGREIPDAPEGADESSLLRL
                     ADGRILCVFRVGSRHEFHRVLSEDEGNTWTRPEAVGGAWSVEPRMVRLENGLIALTGG
                     REGLFLFVCADGEGKTWERINLGAQHNATYPDSAMHFSDVFLEARETPNPPESTAYTG
                     MVAAGPEEVLIAYDRTGNGWDGAPGPRGAVDAVFVVRVRLELAERGERR"
     gene            24025..24573
                     /locus_tag="PLT86_01915"
     CDS             24025..24573
                     /locus_tag="PLT86_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35403.1"
                     /translation="MTAIWSLSLALVACAMVAYLLHRRRKDTPARPDSREGFPVGAGG
                     FTPQPLKPRLQVEDWDGYSGLQAALCVKLTDGTFVPIIEAGTVPPASRIQTLSTARAD
                     QEQVPVVLYSVVPGATNHAALVQELLVGPVPKTGEDIRPVDLVCTLDEDGTVWTRAED
                     TQGHDIPCTVMIRGERRIPVRP"
     gene            complement(24636..25337)
                     /locus_tag="PLT86_01920"
     CDS             complement(24636..25337)
                     /locus_tag="PLT86_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT35404.1"
                     /translation="MFPFFFDPTMLLLLPALAFAIWAQWKVSSTFNRYSRIPSRSRLT
                     GRAVATQILRQNMLNDVEVGHVSGHLSDHYDPTSKTVNLSDSNYASYSVASLAVAAHE
                     VGHALQHAHGYQPVTWRSRLVPVANIGSTAAFPLFFIGLLFGKGLGLFFMDLGIALFL
                     FALLFHIVTLPVEFNASKRALAILEDSGMLAADEIPGAKAVLRAAALTYVASATMALM
                     NLLRLIILRNAMSRD"
     gene            25529..26200
                     /locus_tag="PLT86_01925"
     CDS             25529..26200
                     /locus_tag="PLT86_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT35405.1"
                     /translation="MPKVGILIPAYEAGRTVAAVVAACRGIAGSSDIVVIDDGSTDDT
                     LQNAKNAGAICIRHPENRGKGAALCTGFSEASKLGWDAAITIDADGQHDPAAIPSFLE
                     EYTRCGAGIIIGTRRRTSGMPVVRRASNRLSSSVVSWLAGQRVPDSQSGYRLIAREVW
                     ETVPLSTNRYDMESEMLVRAARRGFAICSVPIPTVYADETSHFHPFRDTARVVRVLWR
                     LWREG"
     gene            complement(26307..27350)
                     /locus_tag="PLT86_01930"
     CDS             complement(26307..27350)
                     /locus_tag="PLT86_01930"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT35406.1"
                     /translation="MRYHRILIAGLASLALCGVASAQSNIGRSGAQFLKISPSARATA
                     LGGAYGSVGRGADAVYHNPAGIAGIRGMNLNLARVNYVADISFNYIAAAMPLGSGALG
                     FQFGSMMMDEMPVTTIQDPTNQLGITFDVGSWVAGITYAQPLTDRLSVGVTGKYVRES
                     IWDMSSSTVALDVGTLYYTGFCNWRFSAVLSNFGRTAKFTGGRLITQYNKYDNPNQSE
                     TVAEDVSMAYELPQAFKLGTAYDFTFTDQMMVTGSLEGAHPIDAPEYLSGGLEYTYAM
                     PQVKLAARAGYRVVQTEAMGSYSSLRLASDSAVLGLGIGVPIAGSNLQFDYTWQNYMK
                     LGNNHLFSVGVSF"
     gene            complement(27381..>28258)
                     /locus_tag="PLT86_01935"
     CDS             complement(27381..>28258)
                     /locus_tag="PLT86_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35407.1"
                     /translation="RFFTGYAGVYEALTGVDWGWGTTVTGVDYAKIEVRFNGGTTRVP
                     VYLRGGSPNYGYVGAGIVPAEIWDVTNNRRLAAVIVEQAGRPEHDMVWAPSLTQAGRE
                     YLFILAQTYDPDTTSAAWTFFRNNPLLANASKFPIMTATWPVARDDGSGAPMTWTLGD
                     VWKINPYLVNTPNDVFEYKTTARVAVDSLVDMSKITVVPNPFIIRHELMPNENNPALY
                     FTNLPPVCTVRIYTLSGDLVTVLQHATSGAAASGTAVWDLRNNNFQRVASGLYLYHVE
                     DASGRTFVGKFAVVR"
BASE COUNT         5768 a         7981 c         8864 g         5645 t
ORIGIN      
        1 cgggacggta tatgtcgcgg tgaccacatt cgacccgaga ctgagggttt ccagcgtgtc
       61 aaccacatag atccggtaac tgtactgcgt gggctcgatc attgcgacgg agtccgtcca
      121 tgagctgacc gccgcgtttg tgatggtgct gacgagcgtg ctggagcgtg tgacggcagt
      181 ggaggtggca cgatagatct cgtaccgggc gaagtcgcct tccgtattgg gggaccaggt
      241 aaggtccacc tggtgcatgg aactggaaac gatcgggttg atctccacgg cacgtgggag
      301 gtcttcgagt cggatgtcgt cgaggtacca gccgtcggct tgccggttga aggaggcatt
      361 cgccgcatca acgttgtcat cgagcaggaa catcacgcgg acgctggggg agccggcgaa
      421 ctgggacaga tcaagctgca cgcgagtcca cgtgggttgg gagccgttgt acgacgcgat
      481 gttcacccat gagccggggc gtccgtaatc ggaagagacg tacacgcggc cccagtcgtc
      541 ttcattgtgg ccgtagctgt ggtcgttgaa gatgttgtac cgatgccaga agaccagggt
      601 cgggtttttc gcgaacgtaa ggtcgaagac tcctgtggag accatgccgt tgtacgcaag
      661 cgccgggtag ttgcctgtct tgctgtcgtg gatcgaggtc ggcccattcg tcccttcggt
      721 ggccggagac cagccgccca gaagccaccg cgacgtgccg gattcgaagt cctcccggag
      781 cgggtatggc attgaggtga gcggtgtctc gttgatggag aaagagtcaa ggtgccagcc
      841 gtcgctttcc acagaactgc tcgcggcgat gcgaaagcgg atccggatac cgtcgtaccc
      901 tgcccactgg gtgaggtcta tacggcgttc ggcccacgtg gtctggctgc cggtgacgga
      961 cgcgacggcg taccagtttg tcccgtccgc ggagacctcc acatggccgt aatcgacgtt
     1021 cgtttcgaac gcgtagcgct cccggtaggt gagcgcgggc atggtggccg tgccaaggcg
     1081 gatggctaac gtcagtgtcc cgcttgagtt cggtgcgtag ttaccgccgg gagagtcgga
     1141 ccagtgccag gatccgctgt acgcgtcgcc ttcacccggg gaattggcga cgatcgccca
     1201 tgtgccggtg ggaacgaagt tcacgttcgc ggcggcaacg gtatcggcga aggggtacga
     1261 gagggcgaga gtacgcgcgc tcggttccgc ggatgaaccg gcgactccgg ccgtgtcgat
     1321 tacaaacacc ttgtagtagt aggtcgtgta ggggaggacg gtgcggtcag caaacgaact
     1381 ggcagttttc gccgagaggg tgtctaccag cgggctggcc gtcgtcacgc cggccgcggt
     1441 gccgcgccgg atttcgtagc gtgcgaagtc gttgccggaa tatggcgtcc atgagagcgt
     1501 gatctcgttg gttcccgtgg cggtggccgt caggttcgtg acactgcctg gagctgcacg
     1561 cgatgttccg ggtgccagga ggatgggcgc agcggaaacc agacacaaca ggaccggcat
     1621 gagcactcga aggcgtcgat cggcggacat ggcgtcaccc cttgtctcga caaaaaggcg
     1681 ccctcgcgca gggtcagata cacatcaccc ggagtcgcgc cccgtactaa gaacctgaca
     1741 caagaatact gatgttgtta atcatcttcg tacgacagac aaaaataccc tgttcgagtg
     1801 atgcgtcaag acgcccggcg cgaaagcccg tttgacgcga agaagcaggg tggtaatcct
     1861 ctctccggta gttctggcgc gccctgccag aacgcgatcc atttgttcac cgtcatcgtg
     1921 caaccctgcg tgacgtcgcg gcatgaacac tgcaaattgc ggcgagaaac gcctctttcg
     1981 cgagcggagg ccacggggag ggaacagccc gtgcgttgat cggggttgtt ttccaaactg
     2041 gacaccacag tgccaccggg gtatgagtac ttttagtatt tgtccctttc cgcttcctgg
     2101 tctgcattcc gggttatcaa gggtcgcatg agcgctgatc agatagtcat ccgtggtgca
     2161 cgagtgcaca accttcgctc ggtggacctg acgctcccga agaaccgtct gatctgtttc
     2221 acgggcgttt cgggttccgg caagagctcg atggcgtttg acactatcta cgccgaaggt
     2281 cagcgcaggt acatcgagtc gctttccgca tacgcccggc aattcatgga gcagatggag
     2341 aagccggacg tagatcagat ctccggcctt cctcccgcga tttcgataga acagaaggcg
     2401 gcgggaggca atccccgaag cacggtagga acgatgacgg aggtgtacga ctacctccgc
     2461 gttctgtacg cacgcctcgg aacactgcac tgcgttcaat gcgggaagcc gatcaccgga
     2521 cagagccggg atcgcctgct tgatcagatt ctgcaacatc cccccggaac acgcctcaac
     2581 atccttgccc cgatggcgcg tggcagacag ggagagtttc tcgacctgtt cgaagagctg
     2641 caacgccagg ggtttgtgcg cgcgcgggtg gacgggcgca ttgtcaatct gagcgaagac
     2701 ccgagcctcg acagacatgc aaagcacaat gttgacgtcg tcgttgacag actggtcatg
     2761 agggaaggga tccgcagcag acttgcggag gcagttgata cggcgctcaa cgtgggaaac
     2821 ggtacgctga tcgtgaacct ggtccgcgac agtgaggacc cgggaaccga catcatcatg
     2881 ggcacggcgt acgcgtgcgc tgaatgcggg atttcttacg aacaaccgac accgcagctc
     2941 ttctcattca acatacccac cggaatgtgc cccacatgcc tgggcctcgg gacgcaggtg
     3001 aagatcaaca gggatctgct ggttaccgat ccctcgaaat cgcttcgcga gggagcgctg
     3061 gcgacggtgg atgtagaccg caaccggtgg ctcaggcatc tgtatcacgg ggtactgcac
     3121 catttcggtg attgcggtct tgacacgcca tggaaagaca ttccggaact cgcaaaggaa
     3181 aaacttctct acgggcttga cgggcaacgc atacgcttcc gttacgcaag gaacccgggc
     3241 gaacggggat ggactcacct cgacacgttc gagggaatca tcccgatgct ggaacgcaag
     3301 ttccgggaat cgaaggatga aggggtgaaa gaggagctgg gcgcattcac ttccgcggtg
     3361 aactgccctg actgcggggg gatgcggctt cgccccgagg ctcgtgccgt gaaactgggc
     3421 gggctgtcgc tgccggaact gtgcgcgatg ccgatcgagg aagcgcacgg gtttctggcg
     3481 acgctgccgc tgtcgtcaac cgagcgccag atcggacagg atgcactgaa cgagattctt
     3541 gcgcggctcg agttcctgat gaacgtgggg ttgcgatatc tctcaatcga ccgcaccgca
     3601 cccacgttat ccgggggaga agcgcagcgc atcaggcttg ccagccagat cggaagtggt
     3661 ctcgtcggtg ttctgtatgt tctggacgag ccttccatcg gtttgcacca ccgggataat
     3721 caccgcctgc tcaatgccct cggacgcctg cgagatctcg ggaacacggt gatcgtcgtc
     3781 gagcacgatg aagacacgat gcgcgccgcc gatatcgtgg tggatttcgg accgggcccc
     3841 ggcgtccggg gcggagccat cgtgggaatc gggtcggccg aggaccttac ggacattccc
     3901 gattcgataa ccggtgacta tctcgcgggg cgcaggagga tagaaatccc ggagacgcgc
     3961 cgtcggccca acggcaactg gctccagatc acgggtgcgc ggcacaataa tctgaagaac
     4021 gtcgacgcaa gaatccccct cggggtgttt gtgtgcgtga ccggagtatc gggcagcggt
     4081 aagagctctc tgataaccga cacgcttttg ccggcgctca tgcggcattt cttccacgcg
     4141 cggttgccgg tggggccgca cgatgcgatc acggggatcg agcatctgga caaggtgatc
     4201 cacatcgacc agagccctat cggaagaacg ccccgctcca accccgcgac ctacaccggg
     4261 gcgctggatc cgatcaggcg tctgttcgcg gagctgcctg aagcgaaagt gcgcgggtac
     4321 acgccgggtc gcttcagttt caacgtgaag agcggacgtt gtgacgcgtg cgaagggaac
     4381 ggagcgactc gcgtccagat ggactttctc gcggacgtct gggttccgtg ccccgcgtgc
     4441 aacggcgcac gattcaacgc ggagacgctg cagatacgtt tcaagggcaa gaacatcgcg
     4501 gacgtactgg atatggacgt acaggacgcg ctccggttct ttgagaacat cccttccgtg
     4561 caccgggttc tccagactct ccacgacgtc ggacttgatt acatgaagct cggccagccg
     4621 tcgcccacgc tctccggcgg cgaggcgcaa cgcatcaagc tggcacgcga actatgccgc
     4681 aggagcacgg ggcggacact ctacattctc gacgagccga cgaccggcct ccatttccac
     4741 gatgtcagcc accttctgga agtgctgcac aggttttgtg acgagggaaa cactgtcgtt
     4801 gtcatcgagc acaatatgga tgtggtgaag accgccgact ggattcttga tctgggcccg
     4861 gagggagggg acgagggtgg acgattgatc gggaccggga cgccggaaga ggtagcagag
     4921 attgatggtt cgtggaccgg gcaggtcctc cgggatgtgt tgcacggcaa ggtgtaccgt
     4981 cgagcaacct cccgagtatc tgaggatgat ggtcattcgc gcgcagcgat cgcgttcgac
     5041 cgggacgaat accgtccgat ggagcggatc acggatatca cggtggtggg tgcacgggaa
     5101 cataacctga agaacgtgtc tctcgctgtc ccacgggaaa aactggtggt tttttcgggg
     5161 gtgtctggtt ctggaaagac atccatggca ctcgacacga tctacgcgga ggggcagagg
     5221 cggtatgttg aatcgctttc cgcatacgca cgccagtttc tcggcgacat tcagaaaccg
     5281 aaagttgatc aggttgaagg gctttcgcct gctatcgcga tcgagcagaa aggagcggga
     5341 cgaaaccccc gcagcacggt cggcacgatt actgagattt acgactacct gcgcgtgatc
     5401 tatgctcaga tcggccaagt gtattgccct gattgccaga tccccgccgg tgctcaatcg
     5461 agcagcgagg ttgtggagcg cctgacggag gaattcaggg gcagacgcat aatgctcctt
     5521 gctccgctgg agacggagaa aggcggagat tacgatgacg cactcgcccg tctgaggaag
     5581 gaggggtatc ttcgttgccg cattgacggg gttgttcacc gtctcgacga agggatcgaa
     5641 ctggacaggc ggaggaatca ctcgcttgaa gccgtaatcg acagacttga gatctcagac
     5701 gatgaacgcg gcaggctggg ggaggcggtt gaaacagcgt tcaacaaggg tgaggggagg
     5761 cttgtagtcg cgccgttgga cggcggggat gatattgcca tcagccggca catatcgtgc
     5821 ccttcgtgcg ggaaaagctt cgagaaactc actccgcaga tgttcgcatt taaccgcgcg
     5881 cacgacatct ctgcgtccgg gatgtgtcct gcgtgccagg gattgggaac ccagcagggt
     5941 ctggcggaag cggcggtcat tcgctcggag aaattgagtg tcgcggaggg cgccgttgat
     6001 ctgtggggca agccgaaggg catgtttcgc gcgcttctgg aggcggcggg gaacgcgctc
     6061 ggtttcgacc ttcacacacc tctgcgggaa atgactcagg ctgcccgaac agccctcttt
     6121 ttcggggatg agagccgatg gatctcgtgc accgaacgcg gcatcgagtt tcagtacctc
     6181 ggcgtgtttc ccgcggtgga tcgcacgttg cggcttacgc cccagctgcg tgatcgcctg
     6241 ggacaggttc tttccgatgt cccgtgcagt acctgcgggg gcacgcggct gattccgcag
     6301 agcagagccg cccgacttcg gggtgtaccc attgcagaac tgtgctcgtg gccgcttgca
     6361 cagacccgcg ccttttttga ccaactgtca ctcagccctc gcgaacagca ggtggtaggg
     6421 gaagtactga ccgagattcg ttctcgcctg cgtttccttg aggaagttgg cctgggatac
     6481 atttcgctgg accggcgggc accaacatta tccggtgggg aagcacagcg cattcggctt
     6541 gcttcgcaga tcggaagcgg gctgaccggt gtgctgtacg ttcttgacga gcctactatc
     6601 gggttgcatc cgcgggacaa cgcgttgctg ctgagtgctc ttgagcacct gcgcgacctg
     6661 aacaacaccg tcattgtcgt ggaacatgac agcgataccc tgaacaaggc ggattacgtc
     6721 gtggacttcg gacccggcgc gggtaattcg ggaggagaag tcgtcgcgac cggtacaccg
     6781 ggagaaatcc ggcagaacca ggcgtcgctc acgggggcat tcctttccgg acgacgaaca
     6841 atacctgttc cacgccagcg cagggcaggc acgggaaagg cgatcgagat catcggagcc
     6901 cgggagcaca atctcaaaga cattcacgcc gtattcccgc tgggaaccat gaccgtggta
     6961 acgggggtat ccggttccgg gaaaagcacg ctggtgaacg acacgctcca tcgggctctc
     7021 gcgtgtgctc tccacaaagc gcagttcatc ccgggcgcgc acgacgaggt gcgcggtacg
     7081 agttttgtgg acaaagtgat actcattgac caggaaccga taggctcctc ttcgctcagc
     7141 aatccggcaa cgtacagcgg cgttttcgac gtactgcgtg aacttttcgc gcaggtgccg
     7201 gaagcgaaga tgcgaggata cacggcgcgg cggttcagca cgaatgtgcc gggcgggcgc
     7261 tgtgaacgtt gctggggcta cggaaaacgg cacattgaga tgcacttcct gccggacgtc
     7321 tgggtggaat gtgaagactg ccacggattg cggtataacc gggagacgct cgagataacg
     7381 ttcggcggcg tgtcgattgg cgaagtgctg cagatgccgg tggaggttgc actggaacat
     7441 ttccagcgtg tgccgcgggt gcggcgtctg cttcagacgc tgtgcgatgt aggcctcggg
     7501 tacatggagc tggggcaatc agccactacc ctgtcgggag gagaagcgca gcggctgaaa
     7561 ctggccagcg agcttgcacg gccttcaacc ggcaggacgg tgtacattct tgacgaaccg
     7621 actacggggc tccacttcgc ggatacggaa cggcttctcg acgtattgaa ccgcctcgtt
     7681 gatcagggga acacggtcat catgatcgag cacaatgttc tggtgattca ggcggctgac
     7741 tggatcatcg atcttggacc cgaaggtggc gacgcgggtg gctacattgt ggttgccggg
     7801 accccggagg atgtggcggc atgcgagagg agccacacgg gcgcggtttt gcgcagggct
     7861 ctggcgcaga aggtgccggc tgtttcgaac aggaaagacg cccgggaagc aatcgcggtg
     7921 gtcgaaaccg aaacacgggg gttggaggag tttcctgagg aggatgttcc gtccggcggc
     7981 cctgcaccgc cctggaaaac agacgggatt gcgtggcaca catccgacaa ccgactcagg
     8041 gaaggccgac agccgctttg gaaaggagcg ctgatcttct ggataattga ccagatcaag
     8101 aagatcgctc ccgtctccgt cgtgtggtcc aatcaacatc tcgtaagtgt gtatccggag
     8161 agagcacgcc gctggatcgc cttccgcaca gactcctggc agtttgtgac gatggaagtc
     8221 cgggttccaa agggctcgtt tgacggggac aaattgcgga aacagcttga tctgaaaccg
     8281 tttgacgagc tcgatgacgt tccgctgtac ggttcgtggt cgcgtgttac gatgaacggc
     8341 cgaatcagaa catggacgat ggtggaaatc gttgccttcc gtcaggagga gcttgataca
     8401 gaaggttttc gcagctttct tcgcgatgcg taccagcttt tcatggacca cgaagcaaag
     8461 ggagggaaag cgaggtccgg ttgacgacat gtggttcgcg acctcgcccg cgtcggaatg
     8521 gacagcaggg aacgtctcct caccgcgatc aacaacggga aacctgatcg cctccctgtt
     8581 caggttcacc gctggatgcc gtactacctc cgcacgtatc tgggaggaat cgatcagtgg
     8641 gcagcctatg agcaattcgg gatggacatg gtgatttact cggacccggt cgtttcagtt
     8701 gatgacacgg aacaatcgcg atggatccgc gaaagagtcc tgtgcgaaac tgaccgggag
     8761 ggtgtccgcc actggaaaat cagggtccgc acgcccgggg gagacctgac gggtgccgaa
     8821 gcagggaacg atatcaccac atggaccacg gaatacctga tcaaaaccga gcgtgacttt
     8881 gaactgtttg agaaatactg cccctcgccg acattcgact ggtctccggt gatgcgagat
     8941 ctgaaccgtc tcgggaaccg cggcattgtc cggactgcgt cgcgtggata cggccaggga
     9001 gcgccgtggc aggacctctg cacgttgatg ggaacggtga gcgccatcat gacggcgctg
     9061 gacgaaccgg aacgtacgag gcacatgctc gacgtgattc tggcgaagag aatctcgacg
     9121 atcgagaagt cgccgccgaa tccgtcggat atcgtatcaa cagggggcgg tgctggttcc
     9181 gatactgtga taagcccgga cctgcacgcg agattctgtc tgccgtacga ccgtgcgtac
     9241 cacgccgcac tgcgagagaa gtcgcctcgc acgaaaatcg tgtatcatct ttgcgggggt
     9301 gttatgaacc tgcttgatct ggttgcaacc aacggcgcgg atggtctgga gacaatgacc
     9361 ccacccagta tgggcggcaa tgcagatctt gccgcggcac gaaggatgat cggcgacagg
     9421 cttttttttg tgggcgggtt cgaccaacag cagggatttg agcgggggac gccggaggat
     9481 gcacggcgtc tggtggtgca gtgtcacgcg gcgtgccctg acgggggata catatgctgc
     9541 ccttccgatc atttctttca cggcaacccg gaaaacatcc gcgcatttgc ggatgcggcg
     9601 cgggaatgca ggtatgatgg ttgacgcaga ccagagcgat cgcggcgtgc cccgtcgagg
     9661 gttcggaggt cttgtggagt gtccttgttg gaggctcgtg tgttttttcg gatcagcacc
     9721 gtaaaatctt tgacaaaaaa agaacttgtg tgcttgcttg acagaaggat tgaggagagg
     9781 tatccttatc tagtgtgaga gactatctgt tatacgcgcg ggggccgaag gcccgcattt
     9841 ctgcgcgatg tgaaggtctt tcgttgttca ggctctgcat gaacggatgc attccgccgg
     9901 ggttggaacg cgctgaaagt tccgcccgga aggaattact gggagtgatt gagggaggga
     9961 accaccaatg agacgtggtt tgctggtgtt ggtcgccggg atagcagtgt gcgccgccgt
    10021 ctgcgcgtcc cccgttcatg ccgtgaaccg gtatccggtg agcataggca tttcgtacgg
    10081 aagctggaca ccctcgatgg acgcgtacaa cgtccgctgg gtggaccaga gcaatccaac
    10141 gatgatcaat gtcggtggca cgctcacccc tgtgtacatc cggcagccgt tggattccgc
    10201 tcagaccatc tgggggaagc gagtaggacc cgagagcttt ctgttctcat cctcgagcgg
    10261 gctgggtctg aacgcgaaac tcaggttgca taatgaccta ttcgcgctcg tggagtacga
    10321 ctcctggaag caatccgtgg gaagtcgccg caattacggc ggcatgatcg gttacgaagc
    10381 gaatgaagtg aagctgaacc ccatcagcgt ttctctgctc tactacatcc cgaccgaagc
    10441 cggcaaatgg tggccgcaca tttacctcgg tgcgggtggc ggatcggtgc tcgtggaacg
    10501 ctcgaattcc cagttgacga atacgggtat cctttcgacg gttacttcta ccggctccgg
    10561 ccctcttttt gtcggaatgg ccggtctcga gtacacggtt cccgctctcc aggaccgatt
    10621 aacgctcttt ttccaagggc gttatactat gggtagcttt gacgaccagt tccccgttct
    10681 gggatcaacc ggttctcagt tgatagaccc ggttaccggc aagcccagga aagaagacgc
    10741 cagcgtgagc atccagggct cgcagatgaa gttcgggctt tcggtgaatt ttggtcagct
    10801 tgtatcgagg ccgacgaagg gcgttctgtc cggttttctg gaagcgaacc gccgccgcgg
    10861 cgggtacgcg atggcgccgt cccgtggtat gcaatcgggc ggttatgcca tgatggttcc
    10921 ctactcttcg gaatcagtgc aggtggtaga aggcgcggga caggttgacg aggaccgcat
    10981 acgtcaggtg atcagagagg agctgcttgg tgcacgaatc ggcgtgactt cagctcgccc
    11041 tgtggatgac ctcgccgagc agcagcttcg cagcattcgc gaaagaaggt tgcaggccga
    11101 gcaggaactc caacagcttc aggagttgtt aagggaagaa ggctgatccc ctcaggatac
    11161 ggggagcggc gcattgcgct gggtaaccct tcttttctcg gctggattgt tgttcggtgc
    11221 ttgggtgccc ggaggggcgt ccgagcactg ttgttctgga gcgctggacc ggctgcatgg
    11281 tgccgggtat gaaaatctcg cgtgcgaaac caccgattcc agtgtcgtcg tgtggtgtga
    11341 aaatcgaaga atccggtacc ctgtcgtctg gatgatcgag gcgttaactg ttgtcgcatc
    11401 ctcggttccg gacgacgtcg tagtgaacgt cgtggcggag cggctggcgg ttcctgtgac
    11461 ctcgctcagt gcccgcgcgg gtgacgtccg cgcatgggtg aagggacgca tcggaacgga
    11521 agaatttcgc caactgctcc gtgtcgagtt cgatgggtcg gaatgtccat ccgaacgtcg
    11581 caactcttca ctgaggaaag tggaccttgc aatcgggccg ggcaggattc tcagtgagtt
    11641 tggtcttccc ggcgtgtgga tccgtgcgaa tttcgacgta agcaccgagt tttcgacggc
    11701 atttcttccc gggttgaatg cttcgggacg tatgttcttc ccggcgtaca acaccggggg
    11761 acctgtttac aacgacagcc gttacaatga gttgcgcccg ggatcggcgt tgttgtcgta
    11821 tctccgcccg gtggggaaga gcggctttct ttccgtctgc ggcggcatgg tggaaatggc
    11881 gaactggcgg tatgattcgt acggtatcgt ggttgatgcg aagcagtatt cgccagatgg
    11941 gacctgggga ttcggcggca gtttcggtta cattgcacca ggaagatatc aggttctgaa
    12001 cgatgccaat cagcgctatc gcacgtgggt tttcgtgtgg ccgctttaca atacgccgta
    12061 cgaagcgtgg accagctaca gggtaaccgg attcgatttg cgcattaccg cccggtgggg
    12121 caggtatctt gtgggtgatc gtgcgtggcg aattgacgtc gagcgttcgt tcggcgaaac
    12181 gaagctcact gtttttggaa tccaatcgaa cgggtactct gattatttga agcgagtaga
    12241 tcgtcagaat acgcggcttc ttggcggggt aaggatggaa ataccattac ccccgcgtga
    12301 acgagggatg ccggaaagat ttcgcgtaac atctacttca gcgttcgcgt ggtcctaccg
    12361 gtaccgcgtg ggcgatgttg gtgtggacct ggtcacgaaa caatcagttg aggaccatgt
    12421 cggggcatac aaccccgtgg ctgttctcaa caatcttgaa cgcgcgcgaa atcgggtgcg
    12481 cgcacggcaa tcagaggcat gccagtgagg aagcaccttt tctccggtgg gggaacaatg
    12541 aaacaaaaaa acagatcgct cgtggtcctt ctggggctgg cgctgacggc aatcggcggt
    12601 ggctgcagcc gcagtgttgt ctcacgcacg caggaaagta actgggtgac accgccacct
    12661 gccacgtcca tcagtgtgac gctgaacgcc ccgacaggtg gtggcgcagg ggacatccag
    12721 ggaacgcgtg tcgttcttat ttcgggtggc agtattcttc gcgatacgac gctggcctcg
    12781 gtgacgacag caccgttcaa attcgcgttc gggaacctgg ccggggtggg aacgtatcag
    12841 gtcgcgatcg tgaaaaccgg gtacacactc caggtgacga cggcttcgat caccggttcc
    12901 gctgtgccgc aggttgtgtt taccctccgg aaactggaaa actctaacac gatctcagtg
    12961 gcaagctcgc tggttacaag tgccggcaca acggtcacta tcccccaacc accggttgtg
    13021 acaggtactt attctaccgc ggctccggca caggtgacac tacccgcgaa ttccgggatc
    13081 agcagtgttt cggttgcgtt gcagaccgcg tcggaagtac ctacgatcag caccacgcag
    13141 tcggtggtaa gcgcggtaca aatacaaacg gacgcgacaa cgacgggaac tgtgacggtc
    13201 aagttcccgc ttcccctgaa ttcgtctgat ttcgccgcgg cagcaggcgg cacggtggat
    13261 gtgcttcgtc tcaaccttac gacgatgacc tgggtgaaaa tcggcgtcgc aaccatcggc
    13321 gctgatggga cggcgacgta caacgttcca gggttgacga acacgaatac tgtcctttgc
    13381 ataggcaaga caccatcgct gtccactgcg gtgcagaccg tcagcacgtc gacgcttctc
    13441 acaccggatc aagtcgcgaa tctggtgatg agcggcgtga caacgaccac ggtgccgttg
    13501 aatgtgtctc tggggcttgg gaaagcgtcg aggagcggcg aagttggcgg cgcggccatt
    13561 gatccgcctg ccggcatgga ccccgcctac tggcaaatgg tgcttggggt attgatggcc
    13621 cggttgccgg agttggagaa ttacttcaaa aacgggacgc agcaggttga ttatcccttg
    13681 cccaaggaac aggccacgtt gcagaaggac cgcaggtcat acacgttcaa tttcacgtac
    13741 acgtggaaca ccggggtttc gaaaacgttc agaattatcg tgacatacga ccatatcaac
    13801 atcaagccga tcacccacac ttcgggtgtg ggtggttgat ttcgtctgaa atggcccgga
    13861 tcgggatttg ttgatctttc acccgcgagc gagctgcccc accggctcgc tcgcgttgtt
    13921 tcaccaccaa ccatctgagg aggccccatg gcgagcgtga acaaagtgat tctcatcggg
    13981 aacctcggca aggatccagt ggtcgatcag acgccctcag ggaccccacg atgccgcttc
    14041 tccctcgcaa cctcggagaa cttcacggac cgagaaggca agaaacagga gcggaccgag
    14101 tggcacaata tcttaatctg gggaaaactg gcggaggttg cgggtcagta tctgcgaaag
    14161 ggcaggccgg tgtatctcga ggggaggatt gcgtacagga gctacgagga tcaggagggg
    14221 aacaagaaga acttcaccga aatcgtagtc acctccatgc agcttcttgg atcgcgctct
    14281 gaccaatcag gatacgacag cgacgttccg ccgccggagg agccgctttc atcgacatcc
    14341 agcacgccac ggcgcaacgc gccaccgcgg aagcagaaag aaaccgggat tgagttgccg
    14401 gaggatatgc ccgccgagga cgaggatctc cccttctgaa ttgcgaacgg ctgactgaga
    14461 gcgtctctcc tatgctgaag ggacgctctc ctcgttattg ttggtgccgt ccctgatttc
    14521 gcgaatcacg cgtgccggat tgcccgcgat taccacgcgg tcggggaaat cgtgggtaac
    14581 caccgcccct gctcccacga ccacaccgtc tcccagagtg accccgggca atatgaccgc
    14641 attcatcccg atccagcagt ctctcccgat cctgatcggc ggccccggca ggtgccgcgt
    14701 gttgtcggtc ggatcatggt tggcgctgat caggccgact cccggaccga tattggtgtt
    14761 gtcaccaatg tagataccgt tcgccgcctg aatgtagcag ccgggagaat ctccggggaa
    14821 ggtgttcttc ccacgcacaa cccgttccgg attcaccacg cgcgacgtga agtgcacccg
    14881 ccagggcaca tgcccgttga tgcgcagaat cctctgcgca atgaatccta tcacatcgtc
    14941 ccgcgtcata cccgagcacc agccgaaagt tgttgccccc gcccgtggcg tttcggtact
    15001 ttcggagtag tcgccgcggg ggattccctt aaagatgatt gtacgcgccg acggcggcaa
    15061 aacccagtcg gtttcgccgg aatttgaacc gaatgcgtaa ggatgaggtt acaggtatgg
    15121 cacgttctgc tgaggtacac cgttccacga aggaaacgaa tgtccgtatt gtgctttcgc
    15181 tggagggcaa cggcgtattc cacggaagca cctccatcgg cttcttcgat cacatgttaa
    15241 cccttttcgc gagacacggc ggcatgagtc tttccctcga ctgcaaaggt gacacgcagg
    15301 tagatggaca tcatacggtc gaggatgtgg ggattgtgct cgggacggca atcgcgcagg
    15361 cactcggcga aaaacgaggc atacgacgct acgcggcggc attcgtcccg atggatgagt
    15421 cgctggcgcg cgtggtggtt gatctttcag gccgcccgta ctgcgtgatc ggcgccgaac
    15481 tcacccccgc gaagattggg gatttcgatg ttgagcttgc gtgggagttc ttccgcgccc
    15541 tgtctgtcca tgccaagtgc aacctccaca tagacctcct gagggccggg aacgcgcatc
    15601 atgcggtgga agcgtgcttc aaagcggcgg cacgcgcatt ctcggaagca tggaccgtcg
    15661 ttggaagcga atccggcgag atcccttcca cgaagggcat tctctgatgg ccgatcgccc
    15721 ggagacggtg cgcatatcgc ttgtcattgt cacctacaat cgtgcggacg agctgattcg
    15781 cctccttcag tcactccaga acgacctcgc ccgtccggac gtggagttaa tcctggtgga
    15841 cgacggatct accgacggca cgctggaacg cgtctcccac ctccttgctc cgattagcga
    15901 tcgcgctact gtgctgcggc aaccgaattc gggacccgga atcggtcgaa atcgcggaat
    15961 actgcgggca tcaggggaga tcgtcgtctt tgtagatact gactgcgttg tccatcctgg
    16021 gtggctttcc gctcttatcg aaccgttttc agacccaaag gtcggcgctg tcggcggacc
    16081 agacaggagc cagccgggtg acccccttct cacaaggttg atcgactacc tgatgacgtc
    16141 ctttctgacc accggcggca ttcgaggggc gaagaagacg cgcggtggat cctatcatcc
    16201 acgctcattc aacatggcag cccgccgtga cgctgcaatt tcggccgaag gattccccac
    16261 catctggtac ggcgaagaca tcctgttcag ccggcgcatt gaacgcgcag gctggcggct
    16321 tgcgttttcg ccggaggcgt gggtgtatca ccgccgccga accactgtcc gcgcgtgggc
    16381 ccgccagctc ttccgtatgg gaagagcccg ctggtggatg ggacgctatg atcgaggcct
    16441 tctcgagccg ctctatgttg ttcctctgct cgaatgctcc gctctcgcac ttgcgattgg
    16501 ggtggtcgca attacgggga atatcgggct gctcaccggg tccttctttg gcgtcgtcgc
    16561 tgcgtatgcc ttggcacttg gcgcagatgc ggcccgccgt acacggcacc cggcggcgtt
    16621 ttttgcggcc cctcttctat ttgcccttcg ggaagcttct tacgcagcgg gctctatcgc
    16681 ggggatattt tcgaaaatcc ccgacctcac cggcgcgctt ccacgcatgg ccgcggagaa
    16741 gcgggagaca gacacatgaa gcctcgatac caccttcgtt tccagatcgc tccgggaccg
    16801 aacctggtcg ctgactcaca gattctggca gatttctgca atcagcacaa tgttgaggag
    16861 gtagtgctgt ttgtcgccgc ggaggaactg aacaacgggt tgctctcgga gacagaggaa
    16921 acgcagtggt tcgacaccat ccgaaccgcg aagcaagttc tcaaaaacgc aggtattgtg
    16981 gtgagcctta atccctggat gacggttctg catactgacc ggggccgcca cttcccgccg
    17041 gatcggacct tcgcgccgat ggtatctccc gaaggggaag tcagcactgc ctgtgcgtcc
    17101 tttgccgacc ctgcctggcg ggactacatc aaacacctct atggccgctt cgccagcctc
    17161 ggatttcgcg tactctgggt ggaagatgat tttcggttcc acaaccacgc gccccttaca
    17221 tggggaggag ggttcgagca acccgtcatc gagcgttttg cggcgaggat tggcaaaccc
    17281 gtgacgcgtg aggagatcgt gaaagcgatt ctcgcgccgg ggaaaccgca tccgtggcgg
    17341 gcaatctgga tggaaacctg gcgggaaatc cacctcgaaa ccgcgcgcat catcgcgaac
    17401 gcagtcgcgg attcgtcccc tgttcagagc acggtcggcc tgatgagctc ccaccccagt
    17461 tcgcacagca ccgaaggacg cgattggaaa gcccttttcg aagcattcac ggtgtcgggt
    17521 cgcgtcgcgc accggcctca tttcgcgccg tacagtgagg caacaggaaa aacgcgtgac
    17581 tggtccttca tgatgctgga cgtccagcgc aatttccgcc cgccgcagag cgaagttgcc
    17641 ccggagatag agaacttccc gttcactcga tggtccaaaa gcgacgctca gacgtggtcg
    17701 gagatggcgc tctgccagtt ttttggatcg gatgcgctgc tgctcgatct gtttccgttc
    17761 acgggcaatt ccgcgaacag ggaaccggaa atcggcgagc tgctcgaccg aagccgtcca
    17821 gcgctggagt ggatcgcctc gcgtttcagc aaagacatgc agaccgaagg gatcggcacc
    17881 gtgtggaagg aagacacgca tgcgcatgtc cggacaactc gcgggacttc catgtccgaa
    17941 ttgaatgcgg atcccttcgt tgccggcaac ttcctgctgc cctacggtgt tgccgcttcg
    18001 atgagaagac agccggtgac cgccctcatg ggcaacgcgg tctgggcact gtccgacgac
    18061 gaaatcgcag gcgcgcttgc gggcggaacg ctggtggatg gggatgcgct ggatattctc
    18121 acccggcggg gattcgcgga gctgctgggc gtgctgtttt ctggatggga agatcgtgac
    18181 cacagcctct attccattga ggaagtggtt tctccccgtg ccggtaccga tgtcgggctg
    18241 gtgttcaaca cgaatgtggt accgcggatt gccagagtgg aggcgatgcc gggtgcggaa
    18301 gaatggacca cgattattac gcccgcacgt cagcgggtcg gggccggcat tgtggccttt
    18361 gagaacaggc ttggcgggcg cgcgataacg ttcgcgacgc ccgggccggc atgcctcccg
    18421 aggagcttcc agcggcagcg aatcgttcac caggcggcga gatttgtctc gagggacact
    18481 ccacttacga tggttacggg cggggcatac ctgatgcccc ttcacctcaa gaacggaggg
    18541 atccaccggg tcgcggtgta caacggttcg ccggaccctg ctcgccctgt tgtctgggat
    18601 agccggacac gcacacggcc acctcgcgtc acgttgcttg cgccacttcg cgatcccatg
    18661 gcgggggacg tgacccccct tcacgcgccg tccggagaga cggggtacga gtgcacgagc
    18721 agcctcccct accacggcta cgcggtcttt gaatgggatg acgcgtaaag gagagagcgg
    18781 gaatgaatga aggattgctg ctttccgatc ttctgacgca tgcagggcgt cccgtcgcct
    18841 cccgtaacga ctggaacgcc aaggcggagg acatccgtgc gcgtatcctg aacgtcctcg
    18901 gggcgttccc tgccgaaact cccgctctgg agcccgaagt ccttgaggaa gttcgcgagg
    18961 acgggtacgt acgcaggctg gtacgttacg cttcggaacc tgacgagcgc gttcccgcgt
    19021 acgtccttct tcctgaaggc cagaagggcc cggcgccggc ggcgctgtgc ctccaccaga
    19081 ctatcgctga agggaaacgc gagaccgtcg ggttgctgca ggacacgcac aaagcatacg
    19141 cgctgcatct tgttcgcagg ggatacgtgt gcattgcgcc ggaccacctt gccgcgggcg
    19201 agaggatcac accgggtctt gacgcgtacg acacctcggc gttctacgcg cggcatccgc
    19261 actggtcggc ggtggggaag gcaatatggg atgcacgccg ggcggtggat tacctgacca
    19321 cgcttccaga agtagatccc gcgcggatag gcgtgatcgg acactcactc ggggggcatg
    19381 gtgccgtttt cgccgcagcg ttcgacgaac gtctgaaagc gtgcgtatcc aactgcggag
    19441 tatcgacgtt ctcaggaagc cccaatcctc ttgcctgggc tcgcgatcgc tggtacgtat
    19501 actttcccgc gctgcgaccc atttttcttg agaaccggcg tcccccgttc gatatgcacg
    19561 aattcgccgc gcttatcgca ccgcgtgcct tcctgaccat cagttctctg actgacgaaa
    19621 gcatgccggt gaacccatac gggcttcagg agttcgccgc tcagctttca cggctgtacc
    19681 agtttgttgg agcgggaggc aagttcgcgt ggttctacca cgatagaggg cattcgtatc
    19741 cgccagaatc gcgcgcgctg gcgtacgaat ggctcgacga aatgctgggg tggcactcca
    19801 ccgaggcata cgacggacgc ccgcggaaat gatgttgtga cctgcgctct cggccttacc
    19861 tgccggcaca ctccggtgcg gccgccgctt cgcctgtttc gataatgata caggggcacc
    19921 ctcgccccac atcacctgca cgcttcacgt tctatgcggt gcacctcttc cattttcctg
    19981 tccagccaat cccggtggac accggcatag ttcaacgatc tcagcctccg ggcgatttcg
    20041 caggcttcgt cgcgtctgcc ctgctgaagg cgaagtttcg cgatccaccg ttcatatttc
    20101 atcacgacga atggccgcgc gcgttcatcc ggggaaaggc cgttcataat ccggccgaaa
    20161 agcgctcccg cgcggacata gtcacccttc tcctggtatg cgcgcgcaac gatttccaga
    20221 aagaaccggc ttgtaggata cttcgcgaca tacggttgta cgagcgcgat cgcatcgtcg
    20281 tagcggcgct ctgacatgta gatccaggcc agcgtttgtt cggcgccacc gtcggaaagg
    20341 acacccatcc gcacggcctg ttccagcatg cgaataccat ccctcccctc ctgtgcagtg
    20401 gcgaacggcc acggaagctt tttcgacttc cagtgcttgt atttccctat ggcaatgagt
    20461 ggatcggcga acgtcgggtc ggccgcacgg gctttttcga gttctgccat ccctgatgaa
    20521 gcgtcggtca gcaggctgcc gatattccgc ccgatacctt ccacgtagct ccggtagagc
    20581 gcccggtacg cgagagcact tcccttgtag aaaagcgcac gtgcgtcgtt cggattccgg
    20641 gcaagatccc gattcgccga ggtgacaacc gcgtcgacct gattcctgaa cgcacgattg
    20701 agcgaatctg cgattccgta gtcatcaatc catgtgaaca gaagcaccag ttcctggaac
    20761 tgcagatcag tggcggccgg atactggcgt tgaagcgcgt tgagcctcgc ctgggcttcc
    20821 gggtagcggt ggataagaat gagatcgctt atatcatttg caactgactg gtatgattgc
    20881 gccaaagcgg tggcggggcc gagcgccaag agcgaagcaa aaagacagac agggacgaat
    20941 cgcgttttca cgtgccgtta ccggaccttt ccaatgaaca tatcgtgatg gcttatttca
    21001 aaatactgga cgcgtttgca gtacgttccc agcaaatcca agaccacatt ccttcccacc
    21061 ggcgccaaga cgcctcctga aagacataca cgccgcaagg gagggggaga ttgcgccgtt
    21121 cacggttcga cgttgttccg gccgctcctt gacaaaaggt aaggaaagaa tatacttaag
    21181 gcgtttggca cacgcgcctg cgcgaaaaag atctgaatcg gcgcgcacta ctacaggtgg
    21241 atcatctcac acacaaaaag ggaggatatt gccatgcgtc tcatcactcg tggcgatctt
    21301 gatggtctga ccagcgcggt tctgatcaca tcgatggaaa agatcgacag tatcgaattg
    21361 gtgcacccga aagacatgca ggacgggaag gtcgaggtta cgggaaatga tatcgtcgtg
    21421 aacctcccct tccatccgaa agcggcaatg tggtttgacc atcatttcgg gtatgatgcc
    21481 caggaagtac cggccaagtt caagggcaaa tacggtgttt cgcccagcgc ggcgcggctt
    21541 gtgttcgagt actacaacca tccaagcctc gacaaatttg gagagctggt aggcgagacg
    21601 gaccgggtcg acgcggcaca actgactctg gacgacattc tccacccgaa gcgctacatt
    21661 ctgctctcct acacgctcga ccctcgcacg ggccttggcg gtgcgttccg ttcctatttc
    21721 atgcaccttc ttgactggct caaggttcac ccaatcggcg aagttctgaa aatgccggaa
    21781 gtcaaggaaa aagttgacac gatcctgaaa aacgagaagg atttccagaa agccttgaag
    21841 aaacactcga agctggatgg gaacattgtg gtgacggact tcaggcgtct ggcggagcgg
    21901 ccggtgggga acagattcgt ggtgtacgcc ctgtaccccg aggcaaacgt gtggatgaac
    21961 cttttcaagg gcaggacagc cgacattacg gtgatctcgc tgggtcacag catcctgaac
    22021 cgtacctgca acacgaatat cggtcagctt ttgagcacat tcggcggcgg cggccaccgc
    22081 ggagccggca cgtgtcaggt cccctcgaaa agcgccggac gcgtcatcaa gaagatcaaa
    22141 gagatactga aagcaaacgg ctgacttcct gcgggagggc gcctgccacg agcgcaggcg
    22201 ccctctccca caaccctgcg ttccctttgt ctccgccatg atacaagcgt tcccgcgcag
    22261 actgtacgaa gctgacaccg cagaagcggc acgccggctg ctcggttgca gactcgttcg
    22321 cgaagttgac ggcgagcgca tggcgggtat cattgtggaa acggaggctt acctcggtac
    22381 agaagacctg ggctgtcact cggcgcgggg tagaaccaac cggaccgagg taatgttcgg
    22441 gccgcccgga cacgcttatg tctacctgat atacggcatg tatcattgcc tcaatgtcgt
    22501 gacacgaccg gaaggcgatc ctcaagcagt cctcatccgc gcgatagaac ccgttgaggg
    22561 actggagcag atgcgcagga acaggagtcg gaggcagcga aaaagctcgc cggttgaccg
    22621 cgaactcgcg aacgggccgg ggaagctctg tgaggcgctg gggattgatg ggagcttcaa
    22681 cggcgttgat ctggcgggca aagccctccg gatcgaactg atgccgcctg ttcccaacca
    22741 ggacatcgcc gctggcccgc gcatcggtat tgcgtacgcg ggggaatggg cggagaaacc
    22801 cctgcgcttc tggatacggg gaaatccctg cgtgtcaacg aagggttgac agcgcgggct
    22861 gaaaggggga ttggccgtga gggtcacata tcagcttccc aattgcaggg tttccgctga
    22921 ccaaccggtt ctcgtcggcg gacaggaagg gcacttctgg ttcccgcacc tgttccgtct
    22981 gagaggaaac cgcattctgt gcgaggtgac ggtaactgcc gacgaggcac agggcgactg
    23041 gccggcggtg ctcttcctct ccgaggacaa aggagcgcac tggcgccgtg ttgcggatct
    23101 ggagactgcc gggccggccg cgaccaggct cggaccggga accatgctcc tcatgccgta
    23161 cgagaactgg ccctccgcac ccggcgacag gcgtaacacg cgcgcgaacg gcaccatcct
    23221 tcgagactcg gcacagggag gactggacgt tgagcccgca gaagtgacgt tccacgggtt
    23281 cccccgggat ctcgcggact accacaaagg cgaggtcgcg ctctttacca acggcaacct
    23341 cctgccaacc agggacggag gcctgctttc gaccgtttac gcaacatttg ccggcgaaac
    23401 gaaatacgat ctgctggcca tggtaagcta cgaccggggg ttttcctggc agttccgttc
    23461 cgtcgccgcg gttggcaggg agataccgga cgcccctgaa ggcgcggatg agtcgagcct
    23521 gctgagactg gcggacggcc gtatcctctg cgtgttccgc gttggaagcc gccacgagtt
    23581 tcaccgcgtg ctgagcgaag acgaaggaaa cacgtggacc agaccagagg ccgttggcgg
    23641 agcctggtcg gtcgaacccc ggatggtaag gctggaaaac ggtctgatcg cgcttaccgg
    23701 cggaagggaa gggttgtttc ttttcgtctg tgccgacggc gaagggaaaa catgggagcg
    23761 catcaatctc ggagcccagc acaacgcaac atacccggac tcggcaatgc atttttcgga
    23821 cgtgtttctg gaagcgaggg aaacgccgaa cccaccggaa agcaccgcct ataccggtat
    23881 ggtcgcggca ggtccggagg aggtgctcat tgcgtatgat cgaacgggca acgggtggga
    23941 tggagcaccg ggccctcgtg gggcggtaga cgccgtgttt gtggtgcgag tccgtctcga
    24001 actcgcagag cggggagagc gacgatgacg gcaatatgga gcctttcact ggcccttgtc
    24061 gcgtgtgcca tggtggcata tctgcttcat cggcggagaa aggacactcc cgcgcgtccg
    24121 gattcccgcg aagggtttcc cgtcggggcc ggtggcttca ctccccagcc gctcaaaccc
    24181 cgattgcagg tggaggattg ggatgggtac agcggcctcc aggccgccct gtgcgtcaag
    24241 ctcaccgatg ggacgtttgt tcccatcatc gaagcaggaa cggtccctcc cgccagccgc
    24301 atccagacgc tttccaccgc ccgggctgac caggagcagg ttcccgttgt tctctattcc
    24361 gtggtgccgg gcgccacgaa ccacgccgcg ctggttcaag agctgctcgt cgggccggtt
    24421 cccaaaaccg gggaggatat tcgcccggtg gaccttgtct gcacccttga cgaggacgga
    24481 acggtctgga cacgtgcgga agacacacag gggcatgaca tcccctgtac cgtcatgatt
    24541 cggggagaac gcagaatccc ggtcaggccc taaaaagaga atcgtgggaa cacagacgca
    24601 ttcccacgat tcccttcgcg cagcggtcgg gagggtcaat cccgcgacat cgcatttctc
    24661 aggatgataa ggcgtaacag gttcatcagc gccattgtgg cagacgcgac gtacgtaagg
    24721 gcagccgccc gcagaacagc cttcgcccct ggtatttcgt ctgccgccag cattccgctg
    24781 tcttcaagaa tcgccagcgc acgcttcgat gcgttgaatt ccacgggcaa tgtaactatg
    24841 tggaacagaa gggcaaaaag aaacagcgcg ataccgaggt ccatgaagaa caatccgaga
    24901 cctttgccga atagaagccc gatgaagaag agcgggaagg ccgccgtcga gccaatgttg
    24961 gcgacaggca cgaggcggga gcgccacgtt acgggctgat acccatgcgc atgctgcaac
    25021 gcatggccga cttcgtgtgc cgccaccgcc aggcttgcca cggagtaact ggcataattg
    25081 ctgtcagaga ggttcactgt ttttgacgtc ggatcgtagt gatcggacag gtgtccgctc
    25141 acatggccga cttcgacatc gttcagcatg ttctgccgga gtatctgcgt cgcaacggca
    25201 cgtcccgtga gccgcgaacg cgatgggatc cggctgtacc gattgaacgt cgagcttacc
    25261 ttccactgcg cccagatggc gaatgcgagc gcgggaagaa gaagcagcat tgtggggtca
    25321 aagaagaacg gaaacatata tttcactcct ggtagccagc ctgcggctgg tattgtccgg
    25381 tgttcacttc caccactatg tatttaccgg gaaccaacgt tgcatgtcaa gccgacacac
    25441 tgtctcatta cgtccagcct gccccgtttg aacgcaaatt ccatgcccgg agttccggag
    25501 tgttcccggt tcgcgaggag ggtaacgcat gcccaaggta ggtattctca tacccgcgta
    25561 tgaagccggg cgcaccgtgg ccgccgtggt ggcggcatgc cggggaattg ccggaagctc
    25621 cgatatcgtt gtcatcgacg acgggtccac agacgacact ctgcaaaacg cgaagaacgc
    25681 cggcgcgatc tgtatccgcc acccggaaaa ccgtggcaaa ggcgcggcgt tgtgcaccgg
    25741 tttctcggaa gcatcgaagc ttggatggga cgccgcgatc acgatagatg cagacggtca
    25801 acatgatccc gccgccattc cctcgttcct cgaggagtat actcgctgcg gcgcagggat
    25861 aatcatcggc acgcgccgac gaaccagtgg gatgccggtg gtccggcgag cctcaaaccg
    25921 gttgtcctca tccgtcgtgt cctggttggc ggggcaacgc gtgccggaca gtcagtcggg
    25981 gtaccgcctg attgcccgcg aggtatggga aaccgttccg ttgtccacca accggtacga
    26041 tatggagagt gaaatgcttg tgcgggcggc gcgccggggt ttcgcgatct gctcggttcc
    26101 cattccgacc gtctacgcgg acgaaacaag tcatttccac ccgttccgcg atacagcgcg
    26161 cgtggtgcgg gtgttgtggc gtctgtggag ggagggatag gagggttgtc gggcagcctg
    26221 tcccgccgca tgaacacagg gacgaacgga aagcggcgag tgtcgtaaga cacccgccgc
    26281 tcttttgttc aggaaacccg gcagaattag aagctcacac caaccgagaa cagatgattg
    26341 ttccccagct tcatgtagtt ctgccatgtg taatcgaact ggaggttgct tccggcaatc
    26401 ggaactccga tgccgaggcc gaggaccgct gaatccgacg cgaggcggag agagctgtac
    26461 gagcccatcg cttcggtctg aacgacgcga taccccgcgc gtgccgcgag cttcacctgt
    26521 ggcatggcgt aggtgtactc cagaccaccg gagagatact cgggagcatc gatcgggtgt
    26581 gcgccttcca gcgacccggt gaccatcatc tggtcggtga acgtgaagtc gtacgccgtg
    26641 ccgagtttga aggcctgcgg cagttcgtag gccatcgaaa cgtcctcagc gacagtttct
    26701 gactggttcg gattgtcata cttgttgtac tgcgttatca ggcgtcctcc ggtgaacttc
    26761 gccgtgcgcc cgaaattgga cagcaccgcg gagaaacgcc agttgcagaa gcccgtgtag
    26821 tacagggttc cgacgtccaa cgcgacggtg gacgaggaca tatcccagat actttcccgg
    26881 acatatttcc ccgtaacacc gacggaaagg cggtcggtga gaggctgtgc ataggtgata
    26941 ccggccaccc acgaaccgac atcaaacgtg atgccgagtt ggtttgtggg atcctggatc
    27001 gtggtaaccg gcatctcgtc catcatcatg gagccgaact ggaagcccaa cgcaccgctc
    27061 ccaagcggca ttgccgcagc gatgtagttg aagctgatgt cggccacata attgacacgg
    27121 gcaagattca ggttcatgcc gcggattccg gcgatgccag ccgggttgtg gtacacggcg
    27181 tcggcacccc tgccgacgga gccatacgcg ccgcccagcg ctgttgcgcg ggcgctcggg
    27241 gagattttta ggaactgcgc ccccgagcgg ccgatgttgg actgcgcgga tgcgactccg
    27301 cacagcgcaa gagaagccag gccagcgatg agtatgcgat gatatctcat ggatgtcact
    27361 ccttccgtca tccgattggc ctagcgcaca accgcgaatt tgccgacaaa cgtgcgtccg
    27421 cttgcgtcct ctacgtgata caggtagagg ccgctggcga cacgctggaa gttgttgttc
    27481 cggagatccc acacggcggt tccgctcgcg gcggcgccgg aagtggcgtg ctggagcacc
    27541 gtgacgagat cacctgacag cgtgtagatg cggaccgtgc acacaggtgg caagttcgtg
    27601 aagtagagcg cagggttgtt ctcattcggc atcagttcat gacgaatgat gaatgggttc
    27661 ggaacgacgg tgatcttgct catgtcgacc agcgaatcca ccgcgacacg ggccgtcgtt
    27721 ttgtattcga acacgtcgtt gggcgtgttg acgaggtacg ggttgatttt ccacacgtcg
    27781 ccgagcgtcc aggtcatcgg cgctcccgat ccgtcgtcac gggcgaccgg ccacgtggcc
    27841 gtcataatcg ggaacttgga cgcgttcgcc agaagcggat tgttgcggaa gaacgtccac
    27901 gctgcagagg tggtatcggg atcgtaagtc tgcgccagga tgaacagata ttcgcgtccc
    27961 gcctgcgtca gactcggcgc ccacaccatg tcatgctctg gacggcctgc ctgttcgaca
    28021 atcacggcgg caaggcgacg attgttggta acgtcccaga tttccgcggg gacgatgccg
    28081 gcgcccacgt agccgtagtt cggtgaaccg ccccggagat acacggggac tctggttgtg
    28141 ccaccgttga agcggacttc gattttcgcg tagtccacac cggtaaccgt cgttccccaa
    28201 ccccaatcca ctccagtcaa cgcttcgtac acgcctgcgt acccagtgaa gaacctgt
//
LOCUS       DAOSOO010000013        27722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1727_length_27722_cov_6.557704, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000013 DAOSOO010000000
VERSION     DAOSOO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 27722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27722
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_27722_cov_6.557704"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLT86_01940"
     CDS             <1..693
                     /locus_tag="PLT86_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT35408.1"
                     /translation="GDTLTSEFVVAGGVEKRYVFRNLSVILNTQRPDSTFIITIPDRL
                     DSSLVGRIELTPSSVDSADEVWLRVNDGDTLRLPADAPRPWAYRFDARLFVRGENRIT
                     VGINSSSNGVQLMRYGVWVSFTQLPVAVEQSQSVLPVRFELEHPRPNPANPATTIGWV
                     QPEACLVRVEVYDVLGRRIRILADRHFSAGRHTVVWDGRDDSGRAVGSSVYLVKARAG
                     NAAQCHKVVISR"
     gene            858..3212
                     /locus_tag="PLT86_01945"
     CDS             858..3212
                     /locus_tag="PLT86_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35409.1"
                     /translation="MNTNRARSGMLLFALAVALSGCDVPKTLTPSVELSTSVPLMDER
                     IEIGEEVAKVDSVQTVGSNPARFVISLNRDRVDLTDGGRIGSDQLRVDDQTPQSITAE
                     MGLISVADVPPTTTPPVVLADLVPDIASMPPGNVPLAIPAYHLPTVKRAVDFSNFQQA
                     TFSSSSDQTLNRVVISLANDGPLPMGPVTIYVSTSGDTMVTSDGSFGTIKDAIAQADF
                     PVIQPGDSVSSTPLDLSGATLRSPAHVFTRAQTQAGTATGNQMLAASFTVTVDISALE
                     VESARAKIPRQTFSRSREVNFASDRIQIYSVKLARSVVADTNRFTLNIVNNLNTPLEV
                     SYDLPDFTIPRTPVTGAQHLQNVSVGSGGASDYARAMIALGARETKTVVFDLSDAVIE
                     NPTAPKTPIDRVRVNLTTTVLGTGDTFVDIVSTDYVQVGADMSALAVQRVEGNVAPSQ
                     PPINVTIQPVDFDVTQNLPAGLTGLAAEEITVELDVTAYETQVDADIGLVLTVANPAG
                     GAPAAQYTKTFSQQFRPGVNRHIRVTQDSLDAFGNSPVDIINAALNNLFASGQTKVEV
                     SGNVNIRGEIVLVRDSSRVDVKDVRVESPLKFTVPQLTFDAKAETEEGFEIDIDESVR
                     NDLVPRARSATLVADVENHFPMGATLVVFASPDPLFSTLRALYPASAKTPVAQIPDTL
                     RIPQGGPVTGEADAIARKAVFKLFTVSLPNPQRLSDGRVDHANPGRATVEVRLDDQLQ
                     LFSYEKLYLMPRIQLLDAPGLVQLSPSDYVKVAIWMQIAGATKR"
     gene            3218..4675
                     /locus_tag="PLT86_01950"
     CDS             3218..4675
                     /locus_tag="PLT86_01950"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOT35410.1"
                     /translation="MDCRKGAYSCAGFLLTALSIAVLPRCSSAATMSFGQARATAMGG
                     AYTAVASGVHAPAWNPANLAFFAAPSAEISLGNLGFFAANDGFSYGDFVGWLDDEVLT
                     TAEIQSALGMFPGGAISIRQTMNVGIPFSVRVKQFAVTGEFMEFVSVGLPRGLFDAIA
                     EDRDATTAYNDARTSGIPYSLSGIVGDVWAVGVTKFSYARRVPLRYADAFSVGATLNY
                     YRASPRVRVVESVGEVLVRPNTWQTDARLVVETGGLSVKRTTDAFGDKQTDVEFEAAN
                     AWGLGCTLGAAGKLDDKTAISAALHNLPLRSITWNMAERRTYTLDSGSRIINAKSLID
                     DKPEGVNTLDYLDTLFAAPGSTVERYERINSMKANVPAYLRVGMARSVDLFPAIWTVD
                     FEQGFSRSALTSTTPRIAAGIEYLPVGPWIPLRAGMSLGGRSGHFASLGMGFHLRWFH
                     WDLALANEGAYTPFEVPFSKRARGLGFASEMKLAF"
     gene            complement(4699..5943)
                     /locus_tag="PLT86_01955"
     CDS             complement(4699..5943)
                     /locus_tag="PLT86_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35411.1"
                     /translation="MGEGDSSSPEELVRRELDPGEVVLWSGAPSPETAFRSGLAYFVA
                     GSVVLFGFSALLTYFLLSATHDLALLVFLVVAGVPATCTAVWWMGTPFRRRHRAGNSI
                     CVLTDRRAIVFDQERSMRVQSFDAGLLAEAEANAGRDGRGDILFPAHVFLPSRKTGQF
                     NRRQAPAALARRGFRGIRDVEHVNALITQVSTRPAQGRAAPCEEVDLRAASLSRVGSD
                     AIRNAIATGEKVLWTGTPAASPVLIRWSPLMVLGGALAAVGIAVAFAPEIFAQSGVFL
                     SNYPVVVRVFGVGFAIAAFGLGLLPVREYRRLRRTVYAVSNRNAYILEAGSSSAVVRT
                     FSAGDVRDHYRRQRPDGSGDLVLQRRLRLEKAKRGAKGPGPEVQLAEEEVGFMGVQDV
                     RGAERCIRAMEHGASQGTQAPR"
     gene            complement(5967..6632)
                     /locus_tag="PLT86_01960"
     CDS             complement(5967..6632)
                     /locus_tag="PLT86_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOT35412.1"
                     /translation="MDAPRNTREHVILELKRAGSLSTKDLAGLLGISSTAVRQILNVL
                     QAEGLIDTRTERQPQGRPCYVYTLTEKGHDLFPEAYDSLAIELLETVREIGGEEMIQD
                     LVAKQVSKKEERYRKLMKGRSLAEKLAQLRELRDGDGYMAVVEEGETKSAIREHNCPI
                     YRIAKNHPEMCAQEIALMERLLGVSIHQTHHMADGQHYCRFEIHKNQASATDPVKEAG
                     LAN"
     gene            6972..8384
                     /gene="glnA"
                     /locus_tag="PLT86_01965"
     CDS             6972..8384
                     /gene="glnA"
                     /locus_tag="PLT86_01965"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOT35413.1"
                     /translation="MTPGDVIKMAHDNGAEVVDLKFIDFPGMWQHFSIPVKFLEPDLF
                     EDGLGFDGSSIRGWQAINESDMLVVPDPNTAVMDPFTAVPTISMICDIHDPITKERYS
                     RNPRNVAKKAEAYLKSTGLADTAYIGPEAEFFIFDDIRYGQTINSGFYHIDSVEGNWN
                     TGRDEKPNLGYKPRLKEGYFPVPPVDHQMDIRTEMMLTMLKCGIEVEAQHHEVATAGQ
                     AEIDMKFCPLVEIADKLMMFKYIVKNVAAKNGKTATFMPKPLFGDNGSGMHVHVSLWK
                     EGKPLFGGNQYAGLSEMALYFIGGLIKHAPSLLAITSPTTNSYKRLVPGYEAPVNLAY
                     SSRNRSAACRIPMYSASPKSKRVEFRCPDPSCNPYLAFAGILMAGLDGILNKIHPGEP
                     LDKDLYDLPKEELAKVPKTPADLAAALDALEADHDYLLKGDVFTSDVIETWISYKRKN
                     EVDALRLRPHPHEFALYFDI"
     gene            8442..8780
                     /locus_tag="PLT86_01970"
     CDS             8442..8780
                     /locus_tag="PLT86_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT35414.1"
                     /translation="MKKIEAFIKPYVLEEVKKALTDVGVKGMSISEVKGFGRQKGHTE
                     LYRGSEYHVDFLPKVKVEVVVSESMVEAAVDAIAKSACTGKIGDGKVFVLPVEKALRI
                     RTGESGEDIL"
     gene            8964..9037
                     /locus_tag="PLT86_01975"
     tRNA            8964..9037
                     /locus_tag="PLT86_01975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8998..9000,aa:Ile,seq:cat)
     gene            9058..9134
                     /locus_tag="PLT86_01980"
     tRNA            9058..9134
                     /locus_tag="PLT86_01980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9092..9094,aa:Val,seq:cac)
     gene            9353..10069
                     /locus_tag="PLT86_01985"
     CDS             9353..10069
                     /locus_tag="PLT86_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT35415.1"
                     /translation="MKQGLFDLLGLQDVDNEIDALQKNKSAYPRRAAELRAEMEDLKQ
                     DRSAKENQLKQHESGFNHFLQQLNIAEENLKKHQTRLQEIKTNREYDAVQQEIIALEH
                     AIEEYFAERDREDEEVRRLRNLLDEERERFEARIAELESEIADLDEKAATIDSEIAKV
                     MEKRKAVAETVPPRILAVYERVRRGKTNAVVRVSRGACGGCWRSLSPQKLNELRMYNR
                     IIACDGCGRIVVWDDRESAG"
     gene            10167..11237
                     /locus_tag="PLT86_01990"
     CDS             10167..11237
                     /locus_tag="PLT86_01990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT35416.1"
                     /translation="MNGVQMDNASEIVRGDSTQPACNEVLVPPSADGIRLDRAIAALL
                     PGISRSQASVAIRDGRVQLFSPKGKVVSHLKAGTPVAAGQTVRIVVARKDPLSVEPEG
                     IDLDIRYEDDDLLVVNKPAGLVVHPAPGHSGGTLVNALAGYSAELSRVGGTFRPGIVH
                     RLDKNTSGLLLVAKNDRAHAALATQLKNRTISREYIALVWGHPSPGSGRIEAAIGRDR
                     AGGKTMSICGRASRAAATRYETISVFSYTSLLLLHLETGRTHQIRVHLKAIGHPVVGD
                     PEYGGREAAIRGIAPAHRSSARFLLTVIGRQALHARRLSFLHPATGKALVVESDPPED
                     FQRALSIVAGAVPQAADFSYFR"
     gene            11399..13657
                     /locus_tag="PLT86_01995"
     CDS             11399..13657
                     /locus_tag="PLT86_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35417.1"
                     /translation="MLTLRNLWCRRGFSAICAIGLCASSFSAVFAQDDREGEPATPYR
                     DAYIAYVDRQLSEIQSQIAFQRSILNERGGELLDEIGIRLQEKLALLPPEMRQPGIPE
                     PPEIVALREYEQTLSNEVVQIEDRILSSADMISEQRDSVAGELKSADAVRATVTELDR
                     SITTDLELWKTKVDLVRSRLIGSGSAGEGRRMFVTEYNALVSHYDAGDYRSARARLTA
                     LATAYGDIPLPEWRERLLFYRAEIDYAMGDWDNAFRGYMEVWQRPRNEFASVALLNWT
                     ELAFAAGEHRKIATMWTAQPRLPEDVVDANRIRVLVAESFLRLNEPQVALSVLNLVQT
                     ALPPAAEDAVDDEETKLANDRLVVYSRLLRAEAHLGGDSGVATLQGAASEETLPEVTD
                     SALDSMLAATGGTPQRGDPNYYSRMAYLIASGQVQAERPGREAGTRVAAAVKELEDVV
                     PLVGPIDPEGTLMARVSMSLGHAYFQANRMEDAARAYGAVPNTSVWYPEAQLGRAWAF
                     IETGNYLDAKIAVNTAQRWPLSPPAALEAAALNSYVLSLIGETELAAEQMREMKSMVD
                     IQQRRAARVWVNQQLQKIDGSLHLVGIIALERQNEALYRSAIEEQASLLALREQVQRV
                     EQYLQSYTPNATGLQDSLTVAIRAEQEALRGLEQKVANLRRAAGQTPAAAANLPEITY
                     EQRREQLQRWLQGVQPQMTPQGSQVYGKWVQYAEFAYSRRVFDENQRRRYEADRLKDV
                     RTRIQKLLSESP"
     gene            13693..15852
                     /locus_tag="PLT86_02000"
     CDS             13693..15852
                     /locus_tag="PLT86_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35418.1"
                     /translation="MRLSRSIAVWRGICGVRAQISSTVCVIALAGLCASSVKAESASP
                     TDTSSLPALLQSSLDGAMTVESVLLGDRRALPPIPRRQFSVGTIPLPPVSPGDTIIVP
                     QVTGEITDPELKERADSLNRSLRLLAIEERLLGSELWGGAPRTGVIPRKDFLEARINL
                     LRDMENVLQIRLREKLAQLDTTIARVESEIEANRTAEIASLEDYLDKYPSSASVADAK
                     FILGQLYYDKERKRYTDAMLRYTTEFQRYRMGLLPVAPAPPVLNEAVSVPFYKSVVEL
                     GTNPDLVPYSLYSLGKFHLEQARDLKARSDESRLTGRREEAQHFTHLSEMETDSAKTY
                     FARLVVEFPDDSVNVPEAFYVLASHYNMLGGPVNRDTAAVYCNALIKNFWYSPRYQNA
                     LVMLGQINYYNGVGTRERAARNRYFSNALAYMAWLARDVDAFTESQIPGVSPDPPRMM
                     DITRRDYAVQFMTQIITRQSPLPGTEPPPPVETAVKLVTAAGNPPFGADLLRQVGDKK
                     NSDYNTSGNPVDLVAALTAYDSLLSRYPTYKEGPQIQQVVIDGATYLSDDPAERYRIY
                     VRQKLLFFERFNRNSEWARQNQDSPAVVKAADDSAAAYLETGARWLYSTARAANDREG
                     VRRSLDYFVKFFQTYPERPQAYELNWSLATELRDLGDYERAYEEFMRVSNADRSQYRE
                     DAALEAVAAAQALVEMEQSGSRSPGRAQETQPPGGGR"
     gene            15855..19892
                     /locus_tag="PLT86_02005"
     CDS             15855..19892
                     /locus_tag="PLT86_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35419.1"
                     /translation="MMTFSRLPKFRVLRSLVGVGLVSLLIGFLVIGGKPSPAPAQFSA
                     EELERNPNLTPSVRRLILAYENYLKHFPDSPQGKEFVIDIAERFRSAGDNETAIVAQK
                     RVLARPDITQAERAYAYEQIMECYRLLGKFDEQEEWARRMAAADVGREKQQQAKDFIF
                     AAGYNRAKALEDSSRLMDAAEAYERLAVMNPDHGQASEAMLKAADLYDTAGDKARAAR
                     TFERFYYTYPDYRDPASGKGALVALENAANLYAEINDYRHTADAAERILAAAPDHPNR
                     QMYMNNLAAIYSLLKDYNNAIRVRQQYVALYPNDPKSGTYQWDIAQFRGLAGQRQQQL
                     AEYEAFIRSYPNDFRAIEANLIVGQDKLAQRDRALARGARDEAERLQALARANFEKAF
                     TLHDSLEALRQGSGDLLHALPAAVEVAKMDSAAYYSISLRGSPSFSQDSTRKWESLRQ
                     AEGMYRKIANYAYPPTTFAALYQRGRLFEDFSAEYLKQPRPDSAVTYDEINKVFFINE
                     VSKQFLQVLAIPAYQTQMLEFYETNRASIDTAAATLPDTSLRTAHLPWLDMARERLAA
                     IPRTLDSLTLNTIAYDADLTVLKAQEKIPQTIAEKWPAFERQQQERYRTDPRLRFGDK
                     QEIFDRGVAPVVYGSGPQDSLALVNYFQRIIADGERLGASRDWVLYNKGRLRLVYGAR
                     SSYFSTVTREGMENLGFQLDTLRQESNGLTNIIARLPKLDLAAIGPPPERPDLTPPAQ
                     PVRPNVDLRAPENRAIALRFIEDFNRFKRQVEDLKYRAVRYQREVERYRARVAELQAS
                     TAAQFRGILEEARTQSQEFSRVATETQRYRMAIELVADRTRNAYERDIAFGDSVGYAE
                     KDRRAVRDSALAANHRISLTLDSLYSEAVATRRYWEERRDSTEGGEASPAFQVINNLV
                     TQYAAVADSFRVTAVRSYMYTYKGRDSIFTVGLDHPIVQYAIQRLRVIDPTFGVQTVP
                     LTFTYVTEDSTGLWRVSSTIDRQNPDAWRQVAFNDSTWVPAVKGTMPRVEFVQAADTT
                     AGAAGQPEPAAGEGAVTAEGDTTAEAAPQVAEVPKIVGFPPDNLNMTDLWSPTYADTV
                     YFRYKLQLPPRWDELPDSLSEPGRKRPVVRKASITITADDDYVVYVNGEVTPARDTDA
                     RVDWKTARTYEVLKDQWTVGDTANIIAIRARNEKRLNRIPDTDTTTYGVMARIDIEMD
                     VPLYIYEALYRPPEPEPTFVLAFTPDDSVKMADTTGTYFRTAAERERWQECRTRTLRA
                     AWEDTVLIPWRAQRDALKKAQLDSEIVRLTRWIRQRQEEARGRIATAAAREAGLEESG
                     ESPAPDAGADSPQETPARPEDTGGP"
     gene            19905..20219
                     /locus_tag="PLT86_02010"
     CDS             19905..20219
                     /locus_tag="PLT86_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35420.1"
                     /translation="MKNPRLVALSISALCTVVLIAPFVGAQEAVRTVRGAGTIREKPP
                     GELATVVRRDPDFRTIKYLGMQRDYRDELRSKLMLGHAVIRHGRPVLPMEPIVWPPEP
                     AQ"
     gene            20411..21235
                     /locus_tag="PLT86_02015"
     CDS             20411..21235
                     /locus_tag="PLT86_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT35421.1"
                     /translation="MGMQSFRKRARKIAYFGVVAGMFMVSYAFGQAAAPAEGAAAPAA
                     GGAPTEQTIDPNIVVKKVVTGFMSDFKPKATGAWLFQWPIVISFACGIGIAAERYYTI
                     VMKSKVNMKKFTDQIIKFVEVKDYDKAIALCNASPSGALPQLVKAGLEAAKRNMGTRA
                     VQDAIDEKTLSILPKITSRTSWVSVLANVSTLLGLMGTIFGLMYTFAGLELVLNPKGK
                     ADLLTSGIATAMNTTLLGLVCAIPLTLLFQYLSQASKAIVDDIDESSVRIINALSR"
     gene            21376..21954
                     /locus_tag="PLT86_02020"
     CDS             21376..21954
                     /locus_tag="PLT86_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35422.1"
                     /translation="MPGFAPSKRSRAEEKETPLNLQVVMNLICILIPALLSMQVQDYY
                     RHDVELPTRGGGGGGPSSGPTNPPFNLKMTIMEDGSFVIVNAKTLSPADGLIPAGAGL
                     AVPPINGEPDYQKLQLVMMKEHKERLGGQPPEAFPDPDQITVSAPGDLDFQHVIKALD
                     RLRFEKPASELDWSSAKQMFTVISLSPGSVGG"
     gene            21985..22461
                     /locus_tag="PLT86_02025"
     CDS             21985..22461
                     /locus_tag="PLT86_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT35423.1"
                     /translation="MPGFPPSKRKAKEEEGGDINITSLMDAFTIILVFLIKQFGTSAV
                     EISAGYRPPVSNTRLAIDRILPLQVRQIGPDAISYQISDKPEKAERKDPNVGYAQLRA
                     DLKAQKPLVDAAVVDEELKGAINIVCDKSVTYATLMDVMKSVAGSGFFKLKLIAEP"
     gene            22497..23654
                     /locus_tag="PLT86_02030"
     CDS             22497..23654
                     /locus_tag="PLT86_02030"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOT35424.1"
                     /translation="MADNHVPPPAQAKAVAAPMVFPPELRRHFYDTADWTSIIFLLVS
                     LTINMLGIGIAASREWKDPAAGLTDEQLMAMQDRIMATFKPVEVKPEEMSAAEQLENL
                     VNNPEFEQALAQQQANLAASLQSTLAEAQATLASLDAQTSAGAAGGADVQGQLDDILG
                     SLGDIGAVGAADAGGPSLDLAPTDVAIFAEGAGSGSRVVSGAIDVAALGSSAKISSAF
                     TRGGLSSGEAARLSQQIAFARERLGGNVQVRVGTGTGGSARRALLRVASGGKTGQRIA
                     VEQLALPPARAAGPGGRDQDAVEVQASRTRQLIGGCYSIGLAADRNLSGVVVVRFTIN
                     PNGSVSGVRVSKSNLGNRDVEECIVAAVQTWKFAPGSTTDTFEYPFSFEPG"
     gene            complement(23752..25044)
                     /locus_tag="PLT86_02035"
     CDS             complement(23752..25044)
                     /locus_tag="PLT86_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35425.1"
                     /translation="MQVRAIIVGAGHRGLGAASYAKHRPEEFAITGVVDPSPLRRERA
                     ASLYDIPAEHCFSTVEELVAYPKCADVAINATMDSLHVPTSLPLLEAGYDILLEKPIS
                     TNIEDLLRLYEAARRLRRTVMICHVLRYAPFYTAIRTRVAAGEIGDIMSVQTVEHVSY
                     HHMAVGFVRGKWRRKDLCGSSMLMAKCCHDLDLIAWMKSGVAPVSVASHGSLMYFRRD
                     KMPEGAGTRCLVDCPIEEGCMYSARKHYIDHPDRWRFYVWDSIEHIAEPTIEDKIESL
                     RTTNPYGLCVWRCDNDVVDHQTVSVEFADGSTAVHSMVGGTARPVRSIHLVGTQGEIQ
                     GVLEDNRFVIRHIDPRPAHEYSEETVDLNVATPELERGGHGGGDRLLVEDFVRFIRGE
                     PRSIAATTLDDSINGHLIGFCADIAREERRVVDIPRIP"
     gene            complement(25050..26021)
                     /locus_tag="PLT86_02040"
     CDS             complement(25050..26021)
                     /locus_tag="PLT86_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT35426.1"
                     /translation="MEFGSVPGVEKPVSRIVLGTMIVTTEEKERSYRLLDDALALGCT
                     TLDTAHVYAGGNSERAIGQWMAERDVRENVVILTKGAHPNADRRRVTPFDIASDLADS
                     LARLRTDYVDIYLLHRDDPAQPVGPIVEALNEHRAAGRIHAFGGSNWTHHRIAEANEY
                     AEAHGLVPFVVSSPNFSLAAQVEDPWGSGSVTLSGPQHADARAWYAGNQIPVFAWSSL
                     ARGFFSGRFTRKTYDAFKDSLDGACRRAYCHEENLERLDRVETLAREKHLTIPQIALA
                     YVMSQPMNVFACVGAADASELGACVEASTVRLTTAEIAWLDLRSESR"
     gene            complement(26036..27037)
                     /locus_tag="PLT86_02045"
     CDS             complement(26036..27037)
                     /locus_tag="PLT86_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35427.1"
                     /translation="MKKKTDDKPVRWGILATGNIAKQFARGLQVIPDARLVAVGSRTR
                     KGAEAFAKEFNVPNVHASYEALASDPDVDAVYVATPHPFHRENTILCLNAGKAVLCEK
                     PFAINAREAREMIACARQNNVFLMEAMWTRYLPLYAKVRELLSNGVIGDVRMVKADFC
                     FRADLKPEGRLFNLALGGGGLLDIGVYTISFANMICGPKPERMASLAHIGTTGVDEQA
                     GLLLSYPGDKLAVLTCAVRTTTPHEAAIFGTEGMIRIHHPFWHGSEMTLSVNGKPDVQ
                     MSHPFVGNGYNCEAVEVMRCIRSGRKESALMPLDESLAIMETMDAFRSEWGLRYPTE"
     gene            complement(27116..>27722)
                     /locus_tag="PLT86_02050"
     CDS             complement(27116..>27722)
                     /locus_tag="PLT86_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35428.1"
                     /translation="KVLRIEAACGDMFDWGTHWFDMVNFYNNDVPAEWIIGQVDSRTE
                     NKVFGVPIENQGIASFRYANGVYGFFVSGYGATIWAEHRILGTDGIIEAHMPVVKVRG
                     RGDTEMRTITFPEQKNIGAWSAITFGIWDLVHGLRTGREPELSARKALAGTQLIFGAY
                     ESSRRRARIEFPLDIDDNPLHSMMASGEIGPGRSEHANVKK"
BASE COUNT         5882 a         8072 c         8187 g         5581 t
ORIGIN      
        1 ggcgatacgc tcaccagcga attcgttgtc gcaggcggcg tggaaaagcg atacgtgttc
       61 cggaatctgt ctgtcatcct gaatacccag cggcctgatt cgacgttcat cataaccatt
      121 cccgacagac tcgacagctc gctggtaggg agaatcgagc ttacccccag ttccgtggat
      181 tccgcggacg aggtctggct cagggtgaac gacggggata ccttgagact tccggcggac
      241 gcgccccgcc cgtgggcgta tcggttcgat gcgaggttgt tcgtccgggg tgagaacagg
      301 attaccgtgg gtatcaattc ttcttcaaat ggcgttcagc tcatgagata tggtgtctgg
      361 gtttccttca ctcagttgcc tgttgccgta gaacaatcgc aatcggttct gccggttcga
      421 ttcgaactgg aacacccgcg tccaaaccct gcgaaccccg ccacaacaat aggatgggtg
      481 caaccggagg cgtgtctcgt gcgtgtggag gtctacgatg ttcttggccg tcggattcgc
      541 atcctcgcgg accgccactt ctccgccgga cggcacacgg tagtctggga tggacgagac
      601 gactcaggac gggcagtggg ttcctctgtc tatctcgtga aagcgcgcgc cgggaatgcc
      661 gcgcaatgcc acaaagtggt catctcccgc tagaagcctt caaggtgtcc atcatctctc
      721 tcccagacaa catcgtctga atgcctgacg gggaaccgac gagtcgcgag attttgacga
      781 gcgtcaggaa cgagatacat ttggggtaat caggggtttg gccgcggaca gaatccagta
      841 acctgaagga gcgcattatg aacaccaacc gggcgcgatc cgggatgctc ttgttcgccc
      901 ttgcagtcgc gctgtcggga tgcgacgtcc cgaaaacgct caccccttcg gtggagcttt
      961 ccacgagcgt accgctcatg gacgagcgca tcgaaattgg cgaagaggtc gccaaggttg
     1021 attcggtgca aaccgttggc tcgaaccctg cccggttcgt catcagcttg aaccgcgacc
     1081 gggtggatct gactgacggt ggccgcatcg gaagtgatca gttgcgggtt gatgaccaga
     1141 cgccgcagtc cataacggcc gaaatggggc tgatcagcgt tgcggatgtc ccgccgacaa
     1201 ccaccccgcc ggtagtgctg gcggatttgg tcccggatat tgcgtcgatg cccccgggca
     1261 acgtccccct tgccatcccg gcttatcatc ttccaacagt aaaaagggcg gtggattttt
     1321 cgaatttcca gcaggccact ttctccagtt cgtctgacca gacgctcaac cgagtggtga
     1381 tttcgcttgc caacgacggg cctttgccca tggggcctgt aaccatctac gtgagcacgt
     1441 ctggcgacac aatggttacc tccgatgggt ccttcggaac catcaaagac gcgattgcgc
     1501 aggccgattt tccagtcatc caaccagggg attccgtctc ctcgactccg ctcgaccttt
     1561 ctggagcaac gcttcgcagt ccggctcatg tctttacacg agcccagacg caggctggca
     1621 ccgcgacggg caaccagatg cttgccgcgt cgtttacggt taccgtggac atttctgcgc
     1681 tggaggtgga atcggcccgc gcgaaaatac cccggcaaac tttttcacgc tcgcgagaag
     1741 tgaattttgc cagcgacagg attcagattt actcggtgaa gcttgcccgt tctgtcgttg
     1801 ccgacaccaa ccgcttcact ttgaacatcg tgaataacct caacactccc ctggaagtgt
     1861 cctacgatct gcccgatttt accatcccgc gcacgccggt taccggagcg cagcatcttc
     1921 agaatgtgag cgtcggctcc gggggtgcct cggattacgc tcgagcaatg atcgcgctcg
     1981 gcgcacgcga aaccaaaact gtcgtgttcg atctcagtga cgcggtcatc gagaacccga
     2041 ctgcgccaaa aacgcctatc gaccgtgttc gcgtgaacct caccaccaca gtgctcggca
     2101 cgggggacac ctttgtagac atcgtttcca cggactacgt gcaggtaggt gccgatatga
     2161 gcgcgttggc ggtgcaacgt gtggaaggga atgtcgcgcc ctcgcaacct ccaatcaacg
     2221 tcacgatcca gccggtcgat ttcgacgtaa cccaaaatct gcctgccggg ttgacaggtc
     2281 tggcggccga ggagatcact gtagaactcg acgtaaccgc gtacgaaacg caggtggatg
     2341 ccgacatcgg gttggtgttg accgtggcaa accctgcggg cggagcaccc gcggcgcagt
     2401 ataccaaaac gttttcgcag cagtttcgac ccggagtgaa ccgccacatc cgtgtgacgc
     2461 aggacagtct tgacgcgttt ggcaactccc cggttgatat cattaatgcc gccctcaaca
     2521 atctgtttgc cagcggccag accaaagtcg aggtctcagg aaatgtgaat attcgcggcg
     2581 aaatagtact ggtccgagat tccagtcgcg tagacgttaa ggacgtacgt gtcgaatccc
     2641 ccctcaaatt cacggttcct caactcacct tcgatgcaaa agcggagacc gaggaagggt
     2701 ttgaaatcga catagacgaa tctgtgcgaa acgatctggt tcctcgcgcc cggagcgcga
     2761 ccctcgtggc ggacgtggag aaccatttcc ctatgggggc caccctcgtc gtgttcgcaa
     2821 gtcctgaccc tctgttctca accctgaggg ccttgtaccc ggcaagcgcg aaaactccgg
     2881 tggcacaaat tcctgacaca ctcaggattc cgcagggtgg acctgttacg ggggaagccg
     2941 acgccatagc cagaaaggcc gtgttcaagc tctttaccgt cagcctcccg aacccgcaac
     3001 gcttgagtga cgggagggta gaccacgcca atcccggccg ggcgacggtg gaggttcggc
     3061 tcgacgacca gttgcagttg ttctcctacg aaaaacttta tctgatgccg cgtatccagc
     3121 ttctcgatgc accgggtctc gttcagttgt ctccttctga ttatgtgaag gttgcaatct
     3181 ggatgcagat cgccggcgcg accaagaggt aacgcccatg gattgcagga aaggcgcgta
     3241 ttcgtgtgct ggctttttgc tgacggcact gtcgatcgcg gtgctgccac ggtgctccag
     3301 cgcggccacc atgtccttcg ggcaagcccg cgcaaccgcc atggggggcg cgtacacggc
     3361 agttgcctcg ggtgttcatg cgcccgcgtg gaatccggcc aacctcgcat tcttcgccgc
     3421 gccgtcagcc gaaatcagcc tggggaacct gggcttcttc gcggcgaatg acggtttctc
     3481 gtacggtgat tttgtcggtt ggctggatga cgaagtgctc accacggcgg aaatccagtc
     3541 ggccctcggg atgtttcctg gcggcgccat cagcatccgg cagacgatga atgtggggat
     3601 accattttcc gtgagggtga aacagtttgc cgtcactggc gagttcatgg aattcgtgtc
     3661 cgtcggtctg cctcgagggt tgtttgacgc gattgctgag gaccgcgacg ccacgactgc
     3721 gtacaacgac gcccgtactt ccggcattcc gtattcgttg tcggggatag tcggtgacgt
     3781 gtgggccgtc ggtgtaacaa agttttccta cgcgcgccgt gtgccgctgc gttacgccga
     3841 cgcgttctcc gtcggcgcga cgctgaacta ctaccgtgcg tcgccccggg tccgcgtggt
     3901 ggaatcagtt ggcgaggtgc ttgtccgtcc gaacacatgg cagacggatg cgcggctggt
     3961 ggtggaaaca ggagggcttt ctgtaaaacg gaccacggac gcgttcggcg acaagcaaac
     4021 ggacgttgag ttcgaagcgg caaacgcctg ggggctcggt tgtacgcttg gcgctgcggg
     4081 gaaactggac gacaaaaccg caatcagcgc ggcgttgcac aatcttccgc tgcgttccat
     4141 tacctggaac atggcggagc ggcgaaccta cacgctggat agcggatcgc ggatcatcaa
     4201 tgcaaaatcg ctcatcgacg acaaacccga aggggtaaac acgctcgatt acctcgatac
     4261 gctgtttgcc gcgccgggaa gcaccgtcga acggtacgaa cggatcaatt ccatgaaagc
     4321 gaacgtaccg gcctatctgc gcgtgggtat ggcgcggtca gtggacctgt tccccgcgat
     4381 ctggacggtt gatttcgagc agggcttctc cagaagcgcc ctgaccagca ccactccgcg
     4441 gatagccgcc ggaatagaat acttgcccgt cggtccgtgg atcccgttgc gcgcaggaat
     4501 gtcactggga ggccgatcgg gccatttcgc gtcccttgga atgggcttcc atctgcgatg
     4561 gttccactgg gatctcgcgc ttgccaacga aggcgcgtac accccgtttg aggtaccctt
     4621 ctccaaacgg gcgcggggac tcggtttcgc gagcgaaatg aaactggcct tctgatacgg
     4681 ggcgctgcca gggcaccctc agcgtggagc ctgggtgccc tgagatgctc cgtgttccat
     4741 cgcgcggata caacgctccg caccgcgcac atcttgtaca cccatgaaac cgacctcttc
     4801 ctcggcgagc tgaacctcgg gacccggccc tttggcgccg cgtttcgctt tctccagacg
     4861 caggcgtctc tgcaacacga gatctccgga accatccggt cgctgccttc ggtagtggtc
     4921 acgaacgtcg cccgcggaaa acgtgcgtac aaccgcggac gaggagcctg cctcgaggat
     4981 gtaggcattt cggttgctca ccgcgtacac cgttcttcgc aatctcctgt attcccgaac
     5041 gggaaggaga cccaagccga acgcagcgat ggcaaaaccg acgccgaaga cccgcacgac
     5101 cacggggtaa ttcgaaagga atacgccaga ctgcgcgaag atttccgggg cgaaggccac
     5161 ggcaatcccc actgcggcaa gcgctccccc gagcaccatc aagggagacc atctgatgag
     5221 aaccggcgac gcagcagggg tcccggtcca caacaccttc tcgccggtgg cgatggcgtt
     5281 tcgaatcgcg tcgcttccca cacgggaaag ggaggcggct cgcaggtcaa cttcctcgca
     5341 cggcgccgct ctgccctgcg ccggtcgtgt tgagacctgc gtgataagcg cgttgacatg
     5401 ctccacatcc ctgattccgc gaaatccccg gcgcgcgaga gctgcgggcg cttgcctgcg
     5461 gttgaactgc ccggttttcc tcgacggcag gaacacatgc gccgggaaca aaatgtcgcc
     5521 acgcccatcc ctgccagcat tcgcctccgc ctccgccaga agaccggcgt cgaacgactg
     5581 gacgcgcatg cttcgctcct ggtcaaacac gattgcccgc ctgtccgtga gaacacagat
     5641 tgagttcccc gccctgtgcc gtcgccgaaa cggggtaccc atccaccaca cggccgtgca
     5701 ggtcgcgggt acgcctgcca cgacgagaaa caccagcagc gcaagatcgt gggtcgcaga
     5761 caggaggaaa tacgtcagga gggcagaaaa accgaacaag acgaccgaac cggcgacgaa
     5821 ataggcaagg cccgaccgga acgcggtctc cggagaagga gcccccgacc agagaacgac
     5881 ctcgcccgga tcaagctcac ggcgaacgag ctcctcaggc gaactgctat ccccctcccc
     5941 cacgggtgct tccccttttc cgaatctcag ttcgcaagtc cggcctcctt gacggggtcg
     6001 gttgcagatg cctggttttt gtgaatctcg aatcggcagt agtgctggcc gtccgccatg
     6061 tggtgcgttt gatgaatcga aacgccgagc aaccgttcca tcaaggcaat ttcctgagcg
     6121 cacatttcgg gatggttttt cgcgatgcgg tatatggggc agttgtgttc acggatcgca
     6181 gattttgttt ctccttcctc cacaactgcc atgtagccat ctccatcgcg cagctctcgc
     6241 agttgcgcca gcttttcagc aagcgagcgc cctttcataa gtttccgata gcgctcctct
     6301 tttttgctga cctgctttgc gacgagatcc tgaatcattt cctccccgcc gatttctcgc
     6361 acggtttcca acagctctat cgccagcgaa tcgtacgcct cagggaaaag atcatgaccc
     6421 ttttcggtca acgtgtacac gtaacaaggg cgcccctggg gctgccgttc agtccttgtg
     6481 tcgatcaggc cttccgcttg cagcacattg agaatctgcc tgacggcagt actggaaata
     6541 cccagaagtc cggccaggtc tttcgtactc aggcttcccg ccctctttag ttccagtatg
     6601 acgtgctctc gggtattcct cggtgcatcc atgcggtttc cttgcggctt tggaatggga
     6661 aagctctgaa gacaatattc tacttgcgaa aatgtgctga gtccagttcc gctaaaggga
     6721 ttcacccgga ggaaaccggc gcacggcact cataacacct ataccgttct tggccgccga
     6781 aaattccgtt gtccgcgttt acgccgattt aacacacgcg caaaaacggc gaaacacata
     6841 acacgtatcc ttttataacg agatcgcagg ggcgggttcg cctcatggtt ctgccggtgg
     6901 tggccaaatg gcgcgaaagc tgccggggct gagatgacac agttcataaa aaggagacaa
     6961 aaccgatggg aatgacgccc ggtgacgtga tcaaaatggc gcatgacaac ggtgcggaag
     7021 tagttgatct gaagttcatc gatttccccg gaatgtggca gcacttcagt atacccgtca
     7081 agttccttga gcctgacctt ttcgaggacg ggttgggctt tgatggctcg tcaatccgcg
     7141 gatggcaggc gatcaacgag agcgatatgc tcgtggtgcc tgatccgaat accgcggtca
     7201 tggacccctt cactgccgtt cccaccataa gcatgatctg cgacattcac gacccgatca
     7261 caaaggaacg ttactcgcgc aatccgcgga atgttgcgaa aaaggcggaa gcgtacctga
     7321 agtcaacagg gctggccgac accgcctaca tcggcccgga ggcggagttt ttcatcttcg
     7381 atgacattcg gtacggccag accatcaatt ccggtttcta tcacatagac tccgtcgagg
     7441 gtaactggaa caccgggcgt gacgagaaac ccaacttggg gtacaaaccg cgccttaagg
     7501 aagggtactt ccccgtgcct cctgttgatc accagatgga tatccgcacg gaaatgatgt
     7561 tgacaatgct gaaatgcggc atcgaggtgg aagcgcagca ccacgaagtt gcaacagcgg
     7621 gacaggcgga aatcgacatg aaattctgcc cgctggtgga gattgcagac aagctgatga
     7681 tgttcaaata catcgtgaag aacgttgcgg cgaagaatgg gaagaccgcc acgttcatgc
     7741 caaaaccctt gtttggggat aacgggtcag gcatgcatgt gcatgtcagc ctctggaaag
     7801 aggggaagcc gctcttcggt gggaatcagt acgcgggcct gagtgaaatg gcgctctact
     7861 tcatcggggg ccttatcaag cacgcgcctt cgcttctggc aatcaccagc ccgacgacga
     7921 actcgtacaa gcggctcgtt ccgggctacg aagcgccggt gaatcttgcg tactcttcgc
     7981 ggaatcgctc cgctgcgtgc cgtataccga tgtattcggc gtcaccgaag tcaaagcgcg
     8041 tggaattcag gtgtcccgat ccgtcctgca acccctatct cgcgtttgcc gggatactca
     8101 tggcggggct cgatggcatt ctgaacaaaa tccacccggg agaaccgctc gacaaagacc
     8161 tgtacgatct cccgaaagaa gaactggcca aggtcccgaa aacgcccgcg gacctcgcgg
     8221 cggcgcttga tgcgctcgaa gccgatcacg actacctgct caaaggggac gtgttcactt
     8281 ccgatgtcat cgaaacctgg atctcataca agcggaaaaa tgaggtcgac gcgctgcgcc
     8341 ttcggcccca tccccatgag tttgcgttgt acttcgacat ctgatacaga cgggtgtttg
     8401 cggcggcggg ccgttcctgg ccttgagaaa ggacgattcc catgaagaag atcgaggcgt
     8461 tcatcaaacc gtacgttctc gaagaagtaa aaaaggctct cacggacgtc ggtgtgaaag
     8521 ggatgtcgat ctccgaagtg aaaggctttg gcaggcagaa agggcacaca gagctttacc
     8581 gcgggagtga gtaccacgtc gattttctgc ccaaagtaaa ggtggaagtt gtcgtgtccg
     8641 aatcaatggt ggaagcggca gtggatgcga tagcgaaatc agcatgcact ggcaagatcg
     8701 gcgatggaaa ggtgttcgtg ctgccggtcg agaaagcctt gcggatccgg acaggggaaa
     8761 gcggggaaga cattctctga gagtcaccaa tgaagaacat gagccgggga gggactctgc
     8821 gccctctccg gcttcttgtt gagagccttc ccgcggtaac cgaccaccgg acctgctgag
     8881 ggaacccgaa ctccgcgcgg cggtcgtacc cttgcctgcc tgcggttgcc tcgtatcttt
     8941 tattcatacg tcaaggattt ttcgccccca tagctcagtt ggttagagcc cccggctcat
     9001 aaccggtctg tcccaggttc aagtcctggt gggggcatta gtaaacattt gttttttggg
     9061 cggttagctc agctggttag agcgctggtc tcacatacca gaggtcacag gttcaattcc
     9121 tgtactgccc accaactctt tgtggatgat actcggtgag gcggactccc ggaagcagtt
     9181 gtcgcccgtt gcggcttgga aaaagcgagc cgaaacgggc gacaccggcg atgtatgatc
     9241 cggaaccgag gggacgcgga aaacggggtg gtattctcga taccaccccg ttttctgttt
     9301 gatccgccgc ctcgcgcaag gtgctcttgg caatggagga tagaaagcga agatgaaaca
     9361 aggattgttc gacctcttgg gattacagga cgtagacaac gagatcgatg cacttcagaa
     9421 gaacaagagc gcgtatcctc gacgtgctgc cgaattgcgc gccgagatgg aggatttgaa
     9481 acaggatcgg tccgccaagg aaaatcagct caagcagcac gaatcggggt tcaaccattt
     9541 ccttcagcag ttgaacatcg cggaagaaaa tctcaagaag caccagacgc gcctgcagga
     9601 aataaaaacc aatcgcgaat acgacgcggt tcagcaggaa atcatcgcgc ttgagcacgc
     9661 gattgaggag tacttcgcgg agcgcgatag agaagatgag gaagtccgca ggttgagaaa
     9721 cctcctggac gaagagcgcg agcgcttcga agcgaggatt gcggaactgg agagcgaaat
     9781 tgccgacctt gatgagaaag cggctaccat tgatagcgaa attgccaagg tcatggaaaa
     9841 gcggaaggca gtcgccgaaa cagtccctcc ccggatactg gcggtctacg aaagagttcg
     9901 gcgtggcaaa accaacgcgg tggtaagggt ttcgcggggt gcctgcggcg ggtgttggcg
     9961 gagcctctct cctcaaaaac tgaacgaatt gcgcatgtac aaccgaatta ttgcctgtga
    10021 cggctgtggc cgtatcgtcg tgtgggatga cagggagtcc gccggatagt tctgagtgac
    10081 tccgcacgaa aaacgatgag atttcggagc gccaacgtga gcagcggtgc tgtttacgcc
    10141 ggcgctttct atcggtggac gtggccatga acggcgttca gatggacaat gccagcgaga
    10201 ttgtgcgcgg tgattcgacg caaccggcgt gcaacgaagt cctggttcct ccctctgcag
    10261 acggtattcg gctcgatcgc gccatcgcgg ctctcctccc cgggatcagt cgttcgcaag
    10321 cgtcggtggc gatccgcgat ggacgggtgc agctcttttc tccgaaaggg aaggtcgtct
    10381 cgcatctcaa agccggcact cctgttgcgg caggccagac cgtgcgcatt gtggttgccc
    10441 ggaaagatcc gttgtccgta gagcctgagg gtattgacct ggacatcagg tacgaagacg
    10501 acgaccttct ggttgtgaac aaacctgcgg gattggtagt ccacccggcc cccggccatt
    10561 ccggcggaac cctcgtgaat gcgctggcgg gttacagcgc cgaactttcc cgggtgggcg
    10621 gaactttcag gccggggatt gtacacaggc tggacaaaaa cacctccggc cttcttcttg
    10681 tggcgaagaa cgatcgcgcc cacgcggctc tcgcaaccca gctcaaaaat cgcacgattt
    10741 cacgcgagta catcgccctt gtgtggggac atccgtcgcc aggttccggg cgcatagagg
    10801 ctgccatcgg acgcgaccgg gcgggtggca aaaccatgtc gatctgcggc agagcgagcc
    10861 gcgcggccgc aacgagatac gagaccatct ctgttttttc ctatacaagt ctgctgctgc
    10921 tccacctgga aacgggaaga actcatcaga tccgcgttca tctgaaggcg atcggacacc
    10981 ctgtggtagg ggacccagaa tacggcggaa gggaagctgc aattcgaggg atcgcccccg
    11041 ctcacagatc ttctgcgcgg ttccttctca ctgtgatcgg gaggcaggcg cttcacgcga
    11101 ggagactgtc ttttctgcat cctgccacag gtaaggcgct cgtggtggaa tccgatcctc
    11161 cggaggattt ccagcgcgca ttgagtattg ttgcgggcgc tgttccacaa gccgcggatt
    11221 tttcttattt tcgataggga gtaccgaatc gaggagcgaa tactgacacc ccgcgcaacc
    11281 cgtcagttga caagagattg tcgttcaagt aatttgtgtg catgcctacg cgcaaaatct
    11341 tggtaggttt gctgcttcgc cacgatagcg cggttcctac cgcggaaaga cgacgggaat
    11401 gctgacactg aggaatttgt ggtgccgcag gggcttctcg gcgatctgtg cgattggcct
    11461 ttgtgcctcc tcattttctg cagtgttcgc gcaggatgac cgcgaaggcg aaccggcaac
    11521 gccctaccga gatgcctaca tcgcgtacgt cgaccggcaa ctctccgaaa ttcagtccca
    11581 gatcgccttc caacggagca ttctcaacga gcggggcggc gaactcctcg atgaaattgg
    11641 aattcgcctt caggagaaac tcgcgctcct tcctccggaa atgcgccaac cggggattcc
    11701 ggaacctcca gaaatcgttg ctttgaggga atatgaacaa actctcagca acgaggtggt
    11761 ccagattgag gacaggatcc tgtcctcggc cgacatgatc tcagagcaac gcgattctgt
    11821 tgccggtgaa ctgaaatcgg cagatgccgt gcgcgccacg gtgacagaac tggatcggtc
    11881 cataacgaca gatctcgaac tctggaaaac gaaggtcgat cttgtgagat cgcgcctgat
    11941 cggttcaggc agcgccggtg aaggccggcg catgttcgtc accgaataca acgcgctggt
    12001 atcccattac gatgccggtg attatcgctc cgcccgcgct cgattgaccg ccctggccac
    12061 ggcatatggc gacattccgc ttcctgagtg gcgcgagagg ctgctgttct accgtgcgga
    12121 aatcgattac gcgatgggcg actgggataa cgccttcagg ggctacatgg aggtgtggca
    12181 acggccgcgg aatgagttcg cttcggttgc cctcctgaac tggacggagc tggcgtttgc
    12241 agcgggtgaa caccgcaaga tcgcaaccat gtggaccgca caacctcggc tgcccgaaga
    12301 cgtggtggat gccaaccgca ttcgcgtgtt ggttgcggaa agcttcctgc gcctcaacga
    12361 gccgcaggtt gccctttcgg tgttgaatct tgtgcagacg gcattgccac ccgcggcaga
    12421 ggatgccgtg gacgacgagg aaacaaaact cgcgaacgat cgcctggtag tctattcacg
    12481 gcttttgaga gccgaagcgc acttgggcgg cgatagcggc gtggcgacac tgcaaggtgc
    12541 tgccagcgag gaaacgcttc ccgaagtcac ggattcggct ctggattcga tgctggcggc
    12601 gacgggaggc actccccaga ggggagaccc gaactactat tcacgcatgg cgtacctgat
    12661 cgccagcggg caggtacagg cggagcgccc cgggcgtgaa gccgggactc gcgtcgcggc
    12721 cgccgtgaaa gaacttgagg atgtcgttcc cctcgtcggt ccaatcgatc ctgagggaac
    12781 gttgatggct cgtgtttcca tgtcccttgg ccacgcgtat tttcaggcca accgcatgga
    12841 ggacgctgcc agggcatacg gggcggttcc gaatacgtct gtgtggtacc cggaagcgca
    12901 gctggggcgc gcgtgggcgt ttatcgagac aggaaactat cttgacgcga aaatcgcagt
    12961 gaacacagcc cagcgttggc cactgagccc ccctgcggct ctggaggccg ccgctctgaa
    13021 cagctatgtg ttatcgctga tcggagaaac tgagcttgcg gctgagcaga tgcgcgagat
    13081 gaagtccatg gtggacatcc agcagcgtcg cgccgcgcgc gtgtgggtga atcagcaact
    13141 ccaaaagatt gatggttctc tgcaccttgt cggcatcatc gcgctggaac gccagaatga
    13201 agcactctac cgttccgcga tagaggaaca ggcgagtctc ctggcgctgc gcgaacaggt
    13261 ccaacgggtg gagcaatacc tccagtctta tacgccgaac gcgaccggtc ttcaagacag
    13321 cctcaccgtc gcgatccgcg cagaacaaga ggcattgcga gggcttgagc agaaggttgc
    13381 gaacctgaga cgtgcggcgg gtcagactcc cgcggccgct gcgaatttgc ctgagattac
    13441 gtacgaacag cgtagagagc agctccagcg ttggctgcag ggtgtccaac cccagatgac
    13501 tcctcaggga tcccaggtgt acggcaagtg ggtgcaatat gcggaattcg cgtattctcg
    13561 gcgggttttt gatgagaacc aacggcgccg ttacgaagcg gacaggctga aggacgttcg
    13621 tacccgcatc caaaaactgc tttcggagtc gccatgaccc accgctttcg atgtgccttt
    13681 caggcgcccc tcgtgcgact gtcgcgttcg attgcggtct ggcggggaat ctgtggtgtt
    13741 cgcgcacaga tttccagcac agtgtgcgtg atcgcgcttg caggcctctg cgcgtcgagc
    13801 gtgaaggcgg agagcgcttc tccgacggat acgagttctc tgccagcgct cttgcagtcc
    13861 agcctcgacg gagcgatgac ggtggaatct gtcctgctgg gtgaccgccg ggctcttccg
    13921 ccaattccgc gccggcaatt cagtgtgggt accattcccc tgcccccggt ttcgcccggc
    13981 gatacgatca tcgttccgca ggtaacgggt gagattacgg atcctgaact gaaggagcgg
    14041 gcagactccc tgaacaggag tctgcggctg cttgcgatcg aagaacgcct gcttgggtcg
    14101 gaattgtggg gtggcgcgcc gcgaacgggc gtgattcctc gcaaagactt ccttgaggcg
    14161 aggatcaatc tcctgcgtga catggagaac gttctccaga tacggctccg ggaaaaactg
    14221 gcgcagcttg ataccaccat tgcccgggtc gaatccgaaa ttgaggcgaa caggacagcg
    14281 gaaattgcgt cgcttgagga ttacctcgac aagtacccga gctccgcgtc cgttgcagac
    14341 gccaagttca tactcggcca gctctattac gacaaggaac gaaagcgcta cacggatgcg
    14401 atgctgcggt acacgaccga attccagagg taccgcatgg ggcttctgcc cgtcgcacca
    14461 gcgcctccag ttctgaacga agcagtatcc gtcccctttt acaaatcggt ggtggaactc
    14521 ggtacgaacc ccgatttggt gccgtactca ctctactcat taggcaagtt tcatcttgaa
    14581 caggcacgtg acctgaaggc gcgatccgat gaaagccggt tgacgggacg ccgtgaagaa
    14641 gcccagcatt tcacacacct gagcgaaatg gaaacggatt ccgccaaaac ctatttcgcg
    14701 cgcctcgtgg tggagtttcc cgacgattcg gtgaatgttc cggaagcgtt ctacgtcctg
    14761 gcctcgcact acaatatgct cggcggaccg gtgaaccgcg ataccgccgc ggtgtactgc
    14821 aacgcgttga tcaagaattt ctggtactca ccgcggtatc agaacgcgct cgtaatgctg
    14881 gggcagatca actactacaa cggtgtcgga accagggaac gcgccgcgcg gaatcgttac
    14941 ttttcgaacg cacttgccta catggcctgg ttggcacgcg atgttgacgc gttcacagag
    15001 tcccagatac ccggtgtgtc tccggatcca ccgcggatga tggatattac gagacgcgat
    15061 tatgccgtgc agttcatgac gcagattatc acgcgccaaa gtccgcttcc cggaacggaa
    15121 cctcctcctc ctgttgaaac ggcggtgaaa ctggttactg cggcaggcaa tcctccgttc
    15181 ggagcggacc tgttgagaca ggtcggagac aagaagaaca gcgactacaa caccagtggc
    15241 aatcccgtgg acctcgttgc tgcgctcacc gcgtacgaca gcctgttgtc ccgctatcct
    15301 acatacaaag aaggtccaca gattcagcag gtggtaatcg acggtgcgac gtacctctcg
    15361 gacgatcccg cggaacggta ccgcatctac gttcggcaga aactgctgtt cttcgaacga
    15421 ttcaatcgaa acagcgaatg ggcgcgacaa aatcaggact cgcctgcggt ggtcaaagcg
    15481 gcggacgatt ccgccgccgc gtatcttgaa acaggcgcgc gatggttata cagcaccgca
    15541 cgcgccgcga atgaccgcga gggcgtgcgg aggagtctgg attacttcgt gaagttcttc
    15601 cagacgtatc cggaacgtcc acaggcgtac gaactgaact ggagccttgc gacagaattg
    15661 cgtgatctcg gcgactatga gcgcgcgtac gaggaattca tgcgggtttc gaatgccgat
    15721 cggagccagt accgcgagga tgctgcgttg gaagccgtgg ctgcggcaca agcgttggtc
    15781 gagatggaac agtccggttc ccggtcaccc ggacgggctc aggaaactca accgcccggt
    15841 ggaggacgct aggcatgatg acgttctcac gccttccgaa attccgcgtc ctccgttcac
    15901 tcgtcggcgt gggtctggtg agccttctca tcggctttct ggtgatcgga ggtaaaccct
    15961 ctcccgctcc cgcccagttt tcggctgagg agctggaacg gaatccgaat cttacgccgt
    16021 cagtccgcag gctcattctc gcctacgaaa actacctcaa gcattttccg gacagcccgc
    16081 agggcaagga atttgtgatc gacatcgccg aacgtttccg ctcggcgggc gataatgaga
    16141 cggcgatagt cgcgcagaaa cgcgtgctgg cccgtcctga tatcacgcag gcggaacgcg
    16201 cgtacgcgta tgaacagata atggaatgtt accgcctgct cggaaagttt gacgaacagg
    16261 aggaatgggc gcgccggatg gcggcagccg atgtcggccg cgagaaacaa cagcaggcga
    16321 aggacttcat cttcgcggcc ggatacaacc gggcaaaggc actcgaggac agctcacggc
    16381 ttatggatgc cgccgaagcg tatgaacgtc tggcggtcat gaatcccgac cacgggcagg
    16441 cgtcggaagc catgctcaaa gccgcggacc tgtacgacac cgcaggtgac aaggcacgtg
    16501 cggcgaggac gttcgaacga ttctactata cgtaccccga ttaccgcgat cctgccagtg
    16561 gaaagggtgc gcttgtcgct ctcgagaacg cggccaacct gtacgcggag atcaacgatt
    16621 accgccacac ggccgatgcc gcggaacgga ttcttgccgc agcgccggac catccgaacc
    16681 gccagatgta catgaacaac ctggcggcga tctacagcct cctgaaggac tacaacaacg
    16741 cgattcgtgt gcggcagcag tacgttgcgt tgtaccccaa cgatcccaaa tcagggacat
    16801 accagtggga tatcgctcaa ttccggggcc ttgcgggtca gcggcagcag cagctcgccg
    16861 aatacgaggc atttatccgg agctatccta acgatttccg cgccatcgaa gcgaatttga
    16921 tcgtcggaca ggacaagctt gcgcagcgtg atcgagccct ggcacgaggc gccagagacg
    16981 aggcggaacg gttgcaggcg ctggcacggg ccaacttcga gaaagcgttc acactgcatg
    17041 attccctcga agccttgagg caaggttcgg gggatcttct gcacgcgctt cccgccgcag
    17101 tcgaagtggc gaaaatggac agtgccgcgt attactcgat ctcattacgc ggttcaccca
    17161 gcttctcgca ggacagcaca agaaaatggg agtcgttgcg gcaagccgaa gggatgtacc
    17221 gcaagatcgc aaactacgcc tatccgccga cgacgtttgc tgcgttgtat caacgtggtc
    17281 gcctgtttga ggacttctct gccgagtacc tgaaacaacc gcgtcccgat tcggccgtca
    17341 cgtacgacga aatcaacaag gtgtttttca taaacgaggt atcaaagcag ttcctgcagg
    17401 tcctcgcaat tcctgcgtat cagactcaga tgctggaatt ctacgagacg aaccgtgcct
    17461 cgatagacac cgcggcggcg acgctcccgg atacatcact cagaacggcg catttgccgt
    17521 ggcttgacat ggcgcgcgag cgacttgccg ccataccgag aacgttggac agccttaccc
    17581 tgaatacgat cgcgtacgac gctgacctga cggtgcttaa ggcgcaggag aaaattccgc
    17641 aaaccatcgc cgagaaatgg ccggcgttcg agcggcaaca acaggaacgc tatcgcacgg
    17701 acccgcgcct gcgtttcgga gacaaacagg agattttcga ccgcggtgtg gcacccgttg
    17761 tctacgggtc ggggccccag gattctctgg cgctcgtgaa ctacttccag cggattattg
    17821 cggatggcga acgccttggc gcctcacggg actgggtgct gtacaacaag ggccgtctcc
    17881 gcctcgtgta cggagctcga agctcgtact tctcgacggt tacgcgggag ggcatggaaa
    17941 acctgggatt ccagcttgat acgttgcggc aggaatcgaa cggcctgaca aatatcatag
    18001 cgcgcctccc gaaactggat ctcgcggcga tcggcccgcc gccggagcgc ccggacctga
    18061 cgccgccggc ccagccggtg aggccgaatg tggacctccg cgctccggaa aaccgggcca
    18121 tagcgctgcg cttcatcgag gatttcaacc gcttcaaacg gcaggtcgag gacctgaaat
    18181 atcgtgctgt ccgataccag cgcgaagtgg aacggtaccg ggcgcgcgtt gccgagctgc
    18241 aggcaagcac ggcggcgcag ttccggggga tactcgaaga agcgagaaca caatcgcagg
    18301 agttttcccg ggtggcgacc gaaacgcaga ggtatcggat ggccatcgaa ctggtggctg
    18361 atcggacgcg gaacgcatac gagcgcgata ttgcgttcgg cgattccgtg ggttacgccg
    18421 agaaggaccg ccgtgccgtc agggacagtg cgctggccgc aaatcacagg atttccctta
    18481 ccttggattc gttgtacagc gaagctgtgg caacaagacg gtactgggaa gaacggcgcg
    18541 attcaacgga aggcggcgag gcctcacctg cattccaggt gatcaataat ctcgttacgc
    18601 agtacgcggc agtcgctgat tcgttccggg tgacggcagt tcgaagctac atgtacacgt
    18661 acaaaggacg cgattcaatc ttcaccgtcg gcctcgatca tccgattgtt cagtacgcga
    18721 tccagcgtct ccgggtgatt gatccgacct ttggcgtcca gacggtgccg ttgacgttca
    18781 cctacgtcac cgaggactcc acaggtctgt ggcgggtcag cagcaccatc gaccgccaga
    18841 acccggatgc gtggaggcag gtcgcgttca atgacagcac gtgggtccct gccgtcaagg
    18901 gaacgatgcc gcgcgttgag tttgttcagg ctgccgatac aaccgccggt gcagcgggac
    18961 agccagaacc ggcggccggc gaaggcgctg tcacagcgga aggcgatacg acggccgagg
    19021 ctgctccgca ggtggcggaa gttcccaaga tcgtcggttt cccgcctgac aacctgaaca
    19081 tgacggacct ctggtcgccg acgtatgccg acacggtgta tttccgttac aagctgcagt
    19141 tgcctccacg gtgggatgag ctgcccgact cgttgtccga accggggcgg aagcggccgg
    19201 tggtgcgcaa agcaagtatc acgattactg cggacgacga ttatgtggtc tacgtgaacg
    19261 gcgaggtgac gccggcgcgc gacactgatg cacgggtaga ctggaaaacc gcgcgcacgt
    19321 atgaggtgtt gaaagaccag tggacagtcg gcgacaccgc aaacattatt gcgatacgcg
    19381 cgcgcaacga gaagcgcctg aatcgtattc ccgacacgga taccacgacg tacggcgtca
    19441 tggcacgtat cgacatcgag atggacgtgc cgctgtatat ttacgaagcg ctgtaccgcc
    19501 caccggaacc ggaaccaacc ttcgtgctgg cgtttacgcc ggacgactcg gtgaaaatgg
    19561 ccgataccac cggtacctac ttccgaacgg ctgctgagcg cgagcggtgg caggaatgcc
    19621 gcacgcgtac cctccgggct gcgtgggagg acacggtgct gattccatgg cgcgctcaac
    19681 gcgatgccct gaagaaggcg caactggact ccgaaatcgt tcggctcacc cgctggatcc
    19741 gtcagcggca ggaggaagca cgcggtcgta tcgcaaccgc ggcggcccgc gaagccgggt
    19801 tggaagaatc gggtgaatcc cccgctcccg acgcgggtgc tgattccccg caggaaaccc
    19861 cggcgcggcc cgaagacaca ggcggaccat aggagttgaa cgtcatgaaa aatccacgcc
    19921 tggttgcgtt gtccatctcc gcgctgtgta cggttgtgtt gatagctccc ttcgtgggcg
    19981 cgcaggaagc tgtgcgaacc gttcgggggg cggggacaat ccgggaaaaa cccccgggtg
    20041 aattggcgac agtggtgcga cgggatccag atttccgcac gatcaaatac ctcgggatgc
    20101 agcgggatta ccgcgatgaa ttgcgctcca agttgatgct tggccacgcg gttatccgac
    20161 acggacgtcc cgtcctgcct atggagccaa tcgtgtggcc gcccgaaccg gcgcagtaac
    20221 gcgcggagaa gcgtcttgac gtgcgggggg tgacaggcta atttcagttc gtcgcggtaa
    20281 ggtgaaaagg atcttgccag aactcgagtg tggtagcggg caggtgtatc agaagtcggt
    20341 cgggcgcggt caaacgggtc ggcggctcgc cgggtgtctt tcataagttt cagtatctac
    20401 ggaggatacg atggggatgc agtccttccg aaaacgggcg aggaaaatcg cctattttgg
    20461 tgtcgtggcg ggaatgttca tggtgtcgta cgcatttggc caggcggcgg ctcctgcgga
    20521 aggcgccgca gcccctgcgg ctggcggcgc cccgaccgag cagacgattg acccgaacat
    20581 cgtcgtgaag aaggttgtta cggggttcat gagcgatttc aaacccaaag ccaccggtgc
    20641 gtggttgttc cagtggccga tcgtgatttc gtttgcgtgc ggtatcggca tcgccgcgga
    20701 acgctactac acgatcgtaa tgaaatcgaa agtgaacatg aagaagttca ccgatcagat
    20761 tatcaagttc gtcgaagtaa aggattacga caaagcgatc gcgttgtgca acgcatcgcc
    20821 ttcgggagcc ctgccgcagc ttgtaaaggc cggtttggag gccgcgaaac gcaacatggg
    20881 aacgcgcgcg gtgcaggatg cgattgacga gaaaaccctc tcgatcctgc cgaagatcac
    20941 atcgcggacc tcctgggtga gtgttttggc aaacgtctcg acacttctgg gtcttatggg
    21001 aaccatcttc ggtctgatgt atacgtttgc cggacttgaa ctggtactga acccgaaggg
    21061 caaggcggac cttctgactt caggcattgc aacagctatg aacacgacgc tgctgggtct
    21121 ggtgtgcgcg attccgctca ccctgctttt ccagtacctg tcgcaggcca gcaaagcgat
    21181 cgtggatgac attgacgaaa gctctgtgcg aattatcaac gcgctgagca ggtgatttgg
    21241 catctgattt gatctctgcg tgcccggcat cctttcctcg aaaaaaggat gccgggggga
    21301 aagacgccca cgggtaatgc gttgttcccg gagcggatcg agatttcgac cttttagacg
    21361 tgtcgaggaa cagcaatgcc aggctttgcg ccatcgaaga gatcgcgtgc agaagagaaa
    21421 gagacgccgc tgaatctgca ggttgttatg aaccttatct gcattctcat tccggcgctc
    21481 ctctccatgc aggtccagga ttactaccgg cacgacgtgg aattgcctac gcggggcggc
    21541 ggcggtggcg gcccgagttc tggtcccacg aaccctccct tcaacctgaa aatgacgatc
    21601 atggaagacg ggtccttcgt gatcgtgaac gccaagacac tctcccctgc cgacggtctg
    21661 attccggccg gggcgggcct ggccgttcct cccatcaatg gcgagcccga ttaccagaag
    21721 cttcagcttg tgatgatgaa agagcacaaa gagcggctcg gcggccagcc accggaagcg
    21781 tttccagatc ctgaccagat caccgtatcc gcacccggcg acctcgattt ccagcacgtg
    21841 atcaaggcgc tggatcgcct tcggttcgaa aaaccggcca gtgagcttga ttggtcgagc
    21901 gcaaaacaaa tgtttaccgt gataagtctt tctcccggta gcgtcggtgg ttgaggaata
    21961 cctcacagcg taaggggtta gcgaatgcca ggcttcccac catcaaaacg gaaagcaaaa
    22021 gaggaagagg gcggcgacat caacatcacg tcgttgatgg acgccttcac catcatcctc
    22081 gtgttcctca tcaagcagtt cggtacgagc gccgtggaga tatccgcggg gtatcgtccc
    22141 ccggtctcca acacccgtct ggccattgat cgcatcctgc cgcttcaggt ccgtcagatc
    22201 ggtccggacg cgatttctta ccagatttcc gacaagccgg aaaaggcgga acgcaaggac
    22261 ccgaatgtcg gatacgctca actacgtgcg gacctgaagg cccagaagcc tctggtggat
    22321 gccgcggtgg ttgacgaaga actcaaaggc gcaatcaata tcgtgtgtga caaaagtgtt
    22381 acctacgcga cgttgatgga tgtgatgaag agtgttgctg gttccggctt tttcaaactg
    22441 aaactcatag ccgaaccata aagtccggtg caaacgttgc tttctgaggt gcaaccatgg
    22501 cagacaacca cgttcctcca ccggcgcaag caaaagcagt ggcagctccg atggtatttc
    22561 cgcccgagct tcgcaggcat ttctacgaca cggccgactg gaccagcatc atcttcctgt
    22621 tggtatcgct caccatcaac atgctgggca ttggaattgc cgcgtcgcgg gagtggaaag
    22681 accccgctgc aggtctgacc gacgagcagc tcatggcgat gcaggatcgc atcatggcga
    22741 cgttcaaacc ggttgaagtg aagccggagg agatgtcggc ggcggaacag ctcgaaaacc
    22801 ttgtcaataa ccctgagttc gaacaggcac tcgctcaaca gcaggcaaac ctcgcggcaa
    22861 gtcttcagag caccttggcc gaagcccagg cgacactcgc ctcgcttgac gcccagacct
    22921 cggctggcgc tgcaggcggc gctgacgtgc aggggcagct tgacgacata ctggggagcc
    22981 tcggcgacat tggagcggtc ggtgccgcag acgcaggtgg tccttcgctt gacctcgccc
    23041 cgacggatgt agcgatcttc gcagagggcg ctggctcggg aagccgcgtg gtttcggggg
    23101 cgattgacgt ggcagcgctc ggttcgtcgg cgaagatatc ctctgcgttt acccgcggag
    23161 gcctttcctc cggcgaagct gcgcgtcttt cacagcagat tgcctttgcg cgcgaacgac
    23221 tgggtggaaa tgtgcaggtg cgcgtcggaa cgggtaccgg cggcagcgcg cgtcgtgcgc
    23281 tgttgcgtgt cgcgtccgga gggaaaaccg gccagcgcat cgccgttgaa cagctggcac
    23341 tcccgcccgc gcgtgcggca ggacccggcg gccgggatca agatgcggtt gaggtccagg
    23401 cctcccgtac tcgtcagctc attggcggtt gctactcgat cggtctggct gcagatcgga
    23461 atctttccgg ggtagtggtg gtccggttca ctatcaaccc caacggctcg gtgagcggcg
    23521 tccgggtttc caaatccaac cttggcaacc gtgacgtcga agagtgcatc gtcgctgctg
    23581 tccagacgtg gaagttcgcg cccggctcaa cgaccgacac gttcgagtat ccgttcagct
    23641 tcgagcccgg ctaatctcac gcaccagaat agaaaaacgc gtccgcggga agaaacacct
    23701 gcggacgcgt ttcattttgt aatctgccct tttgtccgac cgccacgcag atcacgggat
    23761 gcgggggata tccacaacgc gcctttcctc acgcgcaatg tccgcacaga atccgatcag
    23821 atggccgtta attgagtcgt cgagcgtcgt ggcggctatg gagcgcggtt cgccgcgaat
    23881 gaaacgaacg aaatcctcaa caagcagcct gtcgccgccc ccgtgccctc cgcgctccag
    23941 ttccggggtg gccacattca gatccaccgt ttcctctgag tattcgtgcg ctgggcgggg
    24001 atcgatgtgc cgaatgacga agcggttgtc ttccagaact ccctggattt ctccctgcgt
    24061 tcccacaagg tggattgagc gaacaggcct tgccgtgccg cccaccatgc tgtgaacggc
    24121 agtcgatccg tctgcgaact ccacgctcac ggtctgatgg tccaccacat cgttgtcaca
    24181 gcgccacacg cagagcccgt aagggttcgt ggttcggaga gactcaatct tgtcttcgat
    24241 ggttggttct gcgatgtgct cgatgctgtc ccagacgtag aaacgccacc gatctgggtg
    24301 atcaatgtaa tgcttgcgcg cagaatacat gcacccttct tctatggggc aatcaacgag
    24361 gcacctcgtt ccagctccct ccggcatttt gtcgcgacgg aaatacatca gtgaaccatg
    24421 acttgccacg gaaaccggcg ccaccccgct cttcatccac gcgataagat cgagatcatg
    24481 gcaacatttc gccataagca tggaggaccc acataagtcc ttcctgcgcc atttgccacg
    24541 cacaaaaccc acagccatgt gatggtaact tacgtgttca acggtttgca cgctcatgat
    24601 gtcgccgatt tcgcccgccg ccacgcgtgt gcggatggcg gtgtagaacg gggcgtacct
    24661 cagaacgtga cagatcatca cggttcgtct cagtcggcgg gccgcctcgt acagccggag
    24721 aaggtcttca atattcgtag atatcggctt ttccagcaga atgtcgtacc ctgcttccag
    24781 aagcggcagc gaggtgggta catgcagcga atccatggtg gcattaatcg caacatccgc
    24841 acatttggga tatgcgacca gttcctccac cgttgagaag cagtgctccg ccggtatgtc
    24901 gtacaacgac gcggctcgct ctcgacgcaa cggcgagggg tccactacgc cggtgattgc
    24961 gaactcctcc ggacggtgtt tggcgtacga agcggctccc agacctctgt ggccggctcc
    25021 gacaatgatg gctcgcacct gcatgttccc tacctgcttt cggagcgaag gtcaagccag
    25081 gcgatttccg cggtggtcaa acggactgtg ctcgcttcca cacacgctcc gagttcgctc
    25141 gcgtccgcag cgccgacaca ggcgaacacg ttcatcggtt ggctcatgac gtatgccagg
    25201 gcgatctgcg ggatcgtgag gtgcttctcg cgcgcaagcg tttccacccg gtcaaggcgc
    25261 tcgagattct cctcgtggca gtacgccctg cggcacgcac cgtccagcga gtctttgaat
    25321 gcgtcatacg tcttccgagt gaacctcccg gaaaaaaagc cacgggcgag gctggaccat
    25381 gcgaacacgg gaatctgatt cccggcgtac caggcacgcg catccgcgtg ttgcggacca
    25441 ctcagggtaa ccgatccgct tccccacgga tcctcgacct gtgccgcaag actgaaattc
    25501 ggactgctca cgacaaacgg aacaaggccg tgcgcttccg catactcatt cgcttcggca
    25561 atgcggtggt gagtccagtt ggatccgcca aaagcgtgga ttctgcccgc cgccctgtgt
    25621 tcattcagtg cctctacgat cggcccgacc ggctgcgcgg ggtcatcccg gtggagaagg
    25681 tagatatcca cgtagtccgt tcgcagtcgg gcaagcgaat cggcaagatc ggacgcgatg
    25741 tcgaaaggtg ttacccttcg gcggtccgca ttcgggtggg cgccctttgt aaggatgacg
    25801 acgttctccc gaacatcccg ctcagccatc cactggccta tcgcccgttc actgttacca
    25861 cccgcatata cgtgcgccgt gtccagcgtt gtgcaaccga gcgcgagggc gtcatccagc
    25921 aggcggtaac tccgttcttt ttcctctgtc gtaacgatca tcgtaccgag gacaatacgg
    25981 cttacgggct tctcgacgcc gggaaccgac ccgaattcca tgcggtgaat ctccatcatt
    26041 ctgtggggta tctcaggccc cattccgaac ggaacgcatc catcgtctcc atgatcgcaa
    26101 gggattcgtc cagcggcatc agggcactct cttttcttcc cgaccggata caccgcatga
    26161 cctcgaccgc ctcgcagttg tacccgttcc cgacaaatgg atgggacatc tgcacgtcgg
    26221 gtttgccgtt cacggaaagc gtcatctcgc tcccgtgcca gaacgggtga tggatccgaa
    26281 tcatgccctc agttccgaaa atggcggcct cgtgtggtgt ggtggtacga accgcgcatg
    26341 tcaataccgc cagtttgtcg ccggggtaac tcaacagcag ccccgcctgt tcgtcaacac
    26401 ccgtcgtgcc gatgtgcgcg agagatgcca tccgttcggg cttcggaccg cagatcatgt
    26461 tcgcgaacga aatcgtgtac acgccaatat ccagaagtcc accgccacca agtgccagat
    26521 tgaacaggcg accttcgggc ttcagatccg cgcggaaaca gaaatcggct ttcaccatgc
    26581 gtacgtcacc gatcacgccg ttcgaaagaa gctccctgac cttcgcgtac agcggcagat
    26641 agcgcgtcca catggcttcc atcaggaaca cgttgttctg tcgtgcgcac gcgatcattt
    26701 cccgtgcctc gcgcgcattg atcgcaaagg gcttttcaca aagaacagcc ttccccgcgt
    26761 tcaggcagag aatcgtgttc tccctgtgaa aagggtgcgg cgttgcgaca taaaccgcat
    26821 ccacgtccgg gtcgcttgcc aacgcctcgt aagatgcatg gacattgggg acgttgaact
    26881 ccttggcgaa cgcctccgcc ccttttctcg ttctggaacc aaccgcgaca agccgcgcat
    26941 cggggattac ctgcaggccg cgcgcaaatt gcttcgcgat gttaccggtt gccagtatgc
    27001 cccagcgcac cggtttgtcg tccgtctttt tcttcatgtg ttgacctcag aatcgcatag
    27061 atgccggcaa tgaaacaggg aaccgaaaat catcaaacag ggaagaacca tcgtttcact
    27121 tcttcacatt cgcgtgttcg ctccgtcccg ggccaatctc tccagaagcc atcatgctgt
    27181 ggagggggtt atcgtcaata tcgagcggga actctatacg tgcccggcgg cggctggact
    27241 cgtacgcccc gaaaatgagc tgggttcctg ccagcgcctt tcgagccgaa agctccggct
    27301 cgcgtcccgt ccgcagtccg tgaaccaggt cccatatgcc gaacgtgatt gccgaccacg
    27361 cgccgatatt cttctgctcg gggaaggtga tcgtgcgcat ttccgtgtcg cctcttccac
    27421 ggactttcac caccggcatg tgcgcttcaa tgatgccgtc tgttccgaga atgcggtgct
    27481 ccgcccagat cgtcgcaccg taaccgctca cgaaaaagcc atacacgcca ttcgcgtaac
    27541 ggaaactcgc gattccctgg ttctcgatcg gcacgccgaa caccttgttt tcagtccggc
    27601 tgtcaacctg tccgataatc cactctgccg gaacgtcgtt gttgtagaag ttgaccatgt
    27661 caaaccagtg ggtgccccag tcgaacatgt ccccgcaggc ggcctctatt cgcagcactt
    27721 tg
//
LOCUS       DAOSOO010000014        27663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1737_length_27663_cov_5.408106, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000014 DAOSOO010000000
VERSION     DAOSOO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 27663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27663
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_27663_cov_5.408106"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLT86_02055"
     CDS             <1..249
                     /locus_tag="PLT86_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35429.1"
                     /translation="AIGMPGKIWDPKIMAVGERIATAARNAGKMAGTVSVPAKIRALV
                     DMGYNFLNIGSDVGALTEFSNQRVDAFMDSLAGLKANG"
     gene            295..2505
                     /locus_tag="PLT86_02060"
     CDS             295..2505
                     /locus_tag="PLT86_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35430.1"
                     /translation="MDVFAPSAFLDPPSEFRPLQIVHGFDRFLEHPGDLIGEEGIGRR
                     LDQLRRVGIGGVVSNVGGGFGEYLLEERQWEVWVSGMQQAHERGMVLWWYDEKGYPSG
                     TAGGIVTRANPSLCAVGLACYSLSATGPGRFQFVLPASCLKFEVAFASGDFDRVGMDG
                     VIGLTSSTDENGCLYWEVPEGDWTVLYLAQRYMYEGTHAAANVCEFKHYVNLLEPEAT
                     RRFLAVTHDEYFRRTPPGLWKNVRAVFTDEPSLMTGYAGPLPQRFQGNVPVIDKPLFR
                     DRPVAVPWSQGLVAAFTERKGYDPKPRLWCLFCGEHEDARAFRQDFYEIVTELYTNGF
                     YRQVRNWCDRHNIAFSGHVMAEEWLGGHVEYHGSLFAPVREMSLPGIDMLDSDPVSIL
                     EGHGFMTAKQVASIAHLTGAQQVHSECSDWSQRNNGRGATLEQRYGQGGLLYVLGVNQ
                     VTAYWSWDDIGDEAYRTYNDYMGRLGMLLRGGVHRCDVAVLYPIRTAWLDFLPHASYE
                     ADNAGQKVVSTAGARERFNAVGAAYADTVRDLLRSQIDLDIIDEQALTEGIIGAGALH
                     VAGESYRVIVLPQCEAISLSAAEALAAFGRSGGLVVSIGAPPRLVESARATAKVHEGM
                     EQLFGARGNGRILKKEEVVPYLRSARCATLELAEANPSILCTERSRDGRGLCMIVNAS
                     GEPQLIRPRSRLREPLDLYRPLDGSVAPFRAGAELHLGSFESVFLVAHEPSPTV"
     gene            complement(2520..3695)
                     /locus_tag="PLT86_02065"
     CDS             complement(2520..3695)
                     /locus_tag="PLT86_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35431.1"
                     /translation="MLRAGNRAQVYGDDAVIEALHRSGKVAVEDARHWTAGGCMEVSP
                     QGCNCDLLFSFAHNVARTFELVINGGCLMQTGERVVDHPKTLADYTSFEELYTDFEAE
                     LARELGILARRLDIYLEEYARLRPSFLLSSMTHDCLEKGRSINDGGARYTNYGGSGVG
                     IPNVGDSLYAIQRAVFLDRRVTAAELLNALRTDFVGFEALQAYLRSLPKYGAGDEAAD
                     AMTDRVFCTFADLLASHTTRHGGTLVPIILGFVWVVDFGRQVGATPDGRNAGRPLAHG
                     LSPQSGSAVKGITGAIRSATSLSLDKAGGGGAMMWDLDPSWAAPSVVKPLLKTFVDRG
                     GHIFQGNVISVETLRKAQQNPDEYRDLIVRVAGWSARFGTLSPATQEEIITRYKYAG"
     gene            complement(3712..4728)
                     /locus_tag="PLT86_02070"
     CDS             complement(3712..4728)
                     /locus_tag="PLT86_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35432.1"
                     /translation="MQDRLAAFRDQAYARGFASHHSPKEKNYSYAFFDLYADFSLPER
                     QARSLAYALVNEPVLIHPLSRIPGQIYQACAGSACPELSGSTEHPGWVDFSIGQSAER
                     RVAEEFPDGEYYGKYFTQGGYPGHICWDFGRMLDLGIEGMLGLCERGQRQTTAPRSRE
                     FYHCVAIVLQGLLSWVELHVSTIEDLIREETDPVRRKELQEMADVCRRVPRNPSTTFR
                     EAVQTFWFQHLAVMYENPFGGNGPGRLDYYLWPFLERDLASGRIALDEARELVTELLI
                     KLHERIAPADGWVEALPVGGRKPDGSLAINPLSSMIIEIISELPQTHPSVYVRLGRRA
                     RRLC"
     gene            complement(4946..5018)
                     /locus_tag="PLT86_02075"
     tRNA            complement(4946..5018)
                     /locus_tag="PLT86_02075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4983..4985),aa:Lys,seq:ttt)
     gene            5127..5714
                     /locus_tag="PLT86_02080"
     CDS             5127..5714
                     /locus_tag="PLT86_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOT35433.1"
                     /translation="MIRRMYDWVLSWAEHPAGVVALAVLAFSESSFFPIPPDVLLIAL
                     AMGKPKRAFWFAAVCTMGSVAGAMFGYLIGSVFWNAVSPYFFSYVPGFTPRQFEIVRG
                     MYEQYNFWIVFTAAFTPIPYKVITVTAGVFGINFPLFLVGSIIGRAGRFFLVGAMIWR
                     FGPPIKTFIDRYFNALAVAFTVLLVGGFVAIKYAF"
     gene            5748..6824
                     /gene="galT"
                     /locus_tag="PLT86_02085"
     CDS             5748..6824
                     /gene="galT"
                     /locus_tag="PLT86_02085"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT35434.1"
                     /translation="MSGFRKNSVAYSPKQWEMRWHPIRKEWVGFAAHRNTRPWQGEQA
                     PQPPPPPPFDPTCYLCPGNTRNRGERNPDYSGTFVFDNDFPPFGPDAPETTTEHGLYK
                     AMPAYGRCRVVCFHPRHDLTLAEMNREDIRRVVETWQTEHRMLAKDSRINHVLIFENK
                     GSVVGVSNPHPHGQIYATEFVPPVTVTELASSREFYAEKRRTLVGAILEQELRDEVRV
                     IAQNDTSVAFIPYFARYAHETYIVPRRPMPSVADLTPDEADDFTEILHTMLVKFDNLY
                     GISFPYVMAFHQAPTDGAAHPEYHFHVEFHPPLRYPGVLKYLAGAETGAGVFLNDTSP
                     EEKAKEFRAAGNIHYKTTGGQKTA"
     gene            complement(6894..7139)
                     /locus_tag="PLT86_02090"
     CDS             complement(6894..7139)
                     /locus_tag="PLT86_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOT35435.1"
                     /translation="MAKMITCSRTGKVVPQMEKPPMRGKIGQEIWENVSQEAWDEWTR
                     REVMLINEYGLNLADPEDRKVLYENMREFFNLPGKDK"
     gene            7254..8051
                     /locus_tag="PLT86_02095"
     CDS             7254..8051
                     /locus_tag="PLT86_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35436.1"
                     /translation="MNTEHECLIWWEEVHKSFGANHVLRGVTLQVCQGETLVIIGRSG
                     CGKSVLLKTLVGLISPDSGRIWVKDRQIVGDPSAMAEAQRLCGMVFQGAALFDSLSVA
                     ENLALPYWENTDLSKGEIEERIRSLLALVGLRDVEQLMPAELSGGMRKRVSLARALAD
                     EPEIMLYDEPTTGLDPIMADAINELIRSTQDKVKLTSIVVTHDLSTVRKVANHVAMLH
                     EGRIVFHGTPDELFASADPVVTQFIQGRADARVEVPRPELREHEIDE"
     gene            8044..9807
                     /locus_tag="PLT86_02100"
     CDS             8044..9807
                     /locus_tag="PLT86_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35437.1"
                     /translation="MSKPNREERRSASSSWVSWLVEMARAERLWFGGTVLFSVMGALT
                     GALATWLMVPLAAVLFPESTGADAIHNPSRGLYPVSAGAWLMPTIASITPLSVALSFV
                     VVFTVKNLCEFGSRVGCDALVAKVEFRLRNSLWRQYLACGRASGRSFSGARFIHELLA
                     GAGEPAAAVGAIPVRWIGDPVAAVALLGTMAIISPHLTSAMLLLLPPAWWATRKGFRH
                     IGNRTETRLAARGRLGARLSELASFRPVLASHAAMDWAMINTGRDEQSVIRAALSWSR
                     VARLIPLTAETLGAFVGAAVILVVAKGIRAGTLSGGEFLTSLTAFFLLLPVAKRLAAL
                     SGDMRTSRSAWERLASLLPADARAEVAVPPSETFRAPAITLRTLRASIPGGNRVLDIG
                     YAEFPPGEVTAIVGPTGSGKSLLLEIIAGFGVPDEGTVCWNERPISSPHAVPERLPFG
                     YVPQDRWTISGTVGENVTLGRPVSETALTTTLQALGLDLDVQTELGQDGAPLSGGERQ
                     RLALARALLGEPPLLLLDEPTSALDAASEERVIALLRSLKGRTTVLVATHSTAVISAA
                     DWVYHLPFQFATTALHKELVT"
     gene            9807..12590
                     /locus_tag="PLT86_02105"
     CDS             9807..12590
                     /locus_tag="PLT86_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT35438.1"
                     /translation="MRVKMLAAGCVFALSCCTSPRADVTSRYAAQFEEALASMAMSPA
                     DVTFRTDHVERDSFRLRRTDVFLLHPLSFADSLASFSARAGEFGGPSPYVHLAADWLD
                     DPLPPLCEPAARVLVRDTTRCARAFNRLAGAIEHAARIVQESQSVFSPAERESLSTWV
                     DRVLSEGDPDVSGEQVDLFAWNAEELAELQCSRANLELAKRWSGREILRAASCVASAA
                     WELREALMEAKNRVPMPLPVKTWHTTCGRMTVGSDAQDNYTGDFALIVDQSGDDTYRV
                     RPPGSGGGQVQVVIDISGNDVYFGSIAGSTFGIAISLDVSGNDTYIGEAWTQGSARFG
                     AAILWDERGNDTYYANRAVQGTGMFGLGLLVDVDGNDLYRSGTYGQGFGATGGIGILD
                     DRKGNDSYYAGGATTDVLRFDDHYLTMAQGVGSGIRPVGSGGFGFLIDRKGNDVYNAD
                     VYGQGVGYWLGGGGLLDGEGHDRYVAHQYAQGAGIHLSSGALLDFRGDDVYVINGVGQ
                     GCGHDLSVGILFDETGDDAFTVEGLALGAGNANGISVFANVSGRDAYIARREDVMGYS
                     DRRREYGMIGVMLDLGGEDRYAAPLGANDRWWHHSTFGAGVDLGSVSPSSDTATAADL
                     SLTEGGHRQKIAATAESLFVQASNPFSALQYLVEPAENRLADMVGESVTAFWAGKLAS
                     ESARERWALVRIHQKLFARGDLSSVPMLIDSLESPSGAARRMAAHLLGFSKYPDAALP
                     LTGLLRHPDWKTRQVAAESLWRLKDTRSEPALVSLLEDSVALVRHAAALALGTCGTSR
                     SDTALVRRLSDESQIVRYAAEQALGPRATARPLLLQIAGSQGTFAAMHALRALRVDTS
                     DTSYAPVLRSIIRSDGHWAVRAEAANSISALRMQSLSSDLQEVRMDTHHPFLAQRIEE
                     AITTLNGANRRNE"
     gene            12587..13552
                     /locus_tag="PLT86_02110"
     CDS             12587..13552
                     /locus_tag="PLT86_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35439.1"
                     /translation="MNDDFVLKTGENLECRVIDRRNTDLVPALRTLLAHKGDIWQFHL
                     EEEFAGRIKDLETRFYVGLIGSDLVGNIMTVEFKGLGIMGHVYTPPEHRRKGICDVLM
                     DFHMADFRERKGNALYLNTGFESAPFFIYQKHGYRPIPEAPGSMWWSPDSWSPDTLWD
                     DLERLKIAEPAWRHWPSANVFMLQTCLPAVRNVTYGKFGCANAEATFLHVLSDLRNPE
                     KRVQARVLETRDGHMAGLATLAPERRWGSLGTTYVFDLCVHPNAEDVPEQLAEDFQWP
                     AAHVLAYAADDDTAAVNRYAQMGFRPHSHIERFFGTTGLLILSRE"
     gene            13713..14114
                     /locus_tag="PLT86_02115"
     CDS             13713..14114
                     /locus_tag="PLT86_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT35440.1"
                     /translation="MYLHFGAVLAFLLFSAVFVAITYGISALFRPANPSAEKLATYEC
                     GEIPFGGSWIQFNLRYYLIALVFLIFEVEVVFLYPWAVVLRTIGAFAFWEMIVFLAIL
                     LVGFAYVWRKGDLSWFTPSGSGHTDTTFSGK"
     gene            14197..14688
                     /locus_tag="PLT86_02120"
     CDS             14197..14688
                     /locus_tag="PLT86_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOT35441.1"
                     /translation="MGMLEERFDPNIVTTTVDAFLDWARKSSLWPMGFGLACCAIEMM
                     ATSMSRYDLDRFGASVFRSSPRQCDLMIVSGTVTLKMASRVKRLYDQMAEPRYVISMG
                     SCANTGGPYWEHGYHVLKGVDRIIPVDVYIAGCPPRPEALIDGVLQLQKKITEQRVVR
                     KAV"
     gene            14696..15262
                     /locus_tag="PLT86_02125"
     CDS             14696..15262
                     /locus_tag="PLT86_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT35442.1"
                     /translation="MDALAIATMVKDVFGGAVLAVDQPEVEPVAVIEPANVAEVARFL
                     RETPELAFDYLMCLSGFDTMPTEEDHPARNTPEEDTGAPSTETAAPALGVAYHLFSYS
                     HSHRFAFKAHTPRENPHIPSVAEIWPAADWHEREAFDLFGIIFDGHPDLRRILLPEDW
                     VGHPLRKDYAAQESYRIGDACAQIARGW"
     gene            15287..16402
                     /locus_tag="PLT86_02130"
     CDS             15287..16402
                     /locus_tag="PLT86_02130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOT35443.1"
                     /translation="MPLKTEEMLLNVGPQHPSTHGVLRLEVTTDGELVTSAEPTIGYL
                     HRCFEKYAETLTYQQIIPYTDRLDYLSSMNNNFGFVVAVEKLMGIHVCERAERIRVIM
                     AELNRIASHLMFIGSFSNDLGAMTPFLWCFRDREEILKIFEEVSGARLLYNYYRIGGL
                     AADLTPGIEEKIYSFLNQFEAHMLELDDLFTRNAIVIARLKGVGMIPREMAIAYGLSG
                     PMLRASGVDWDCRRDEPYSIYASLRFEVPVGGEKLGALGDCYARYLVRRAEIDQSVSL
                     VRQCLADIPPGDVKEQVKRNVRPPVGEVYVRTENPRGEIGFYLRSDGSTKPSRVKVRA
                     PSFCNMSVFSRISKDILVADMIAILGSVDIVLGEIDR"
     gene            16422..17588
                     /gene="nuoH"
                     /locus_tag="PLT86_02135"
     CDS             16422..17588
                     /gene="nuoH"
                     /locus_tag="PLT86_02135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOT35444.1"
                     /translation="MQEMYETLLAALGAERVIPFSVGFAVLAVVFALFAAIMGAVVAL
                     IMVLAERKISADIQERIGPNRVGPMGFFQTVADAVKLLTKENVLPRKADSILHYCAPT
                     LVFTGALLPVAAIAWSNRIVAADLNIGVLYVVGTASLGVIGRLMAGWASNNKWSLLGG
                     MRAAGQMISYEIPNSIAILSVIVLAGTLNLQELSEAQMGGIHQWFVFRSPFAFVACLI
                     YLLSSLAEVNRTPFDLPEAESELVSGYHTEYSGIRWAIFMMSEYIGMVVVNAVAVALF
                     FGGWNGGLRGEPLLGGWIWYLLKIAVLMYCQVWIRFVLPRLRIDQLMSLCWKNLVPLG
                     FLALLGASVQALMGPTGQRVMMVAAWLVFGGFVTLLFVAVRKTRHVPAQGGFSG"
     gene            17585..18283
                     /locus_tag="PLT86_02140"
     CDS             17585..18283
                     /locus_tag="PLT86_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOT35445.1"
                     /translation="MRAFFGYWRGIASGFMTTLVGMVAAIRHVGRKEYTLEYPERRDV
                     LPPRARMSLFMNADECIACAQCARACPVNCITVASVKRPKDEEPPVTVVGQRKKVLVL
                     TGYKIDMGRCCFCGLCVEPCPTHSLYMTPYFEMAVVQKAAEPDEILHEGESFAAADAR
                     RTRSEIQVQHPRFGRTGLVYDFLAITDDLRRRGETVPVNPLFPEEHAQLVQNRVRPPN
                     QDAAGTTDPPGGRN"
     gene            18280..18795
                     /locus_tag="PLT86_02145"
     CDS             18280..18795
                     /locus_tag="PLT86_02145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT35446.1"
                     /translation="MNAYDLAFYLLAAMALVGAAITVLSRRLVYAAFSLLLAFLGVAG
                     LYALLAADFLAIAQVVVYVGGILVLLLFGVMLTHRIYDTTLKSVRVQPLLGGLVAAGI
                     AILLISVAWNTPWNTTHEIAAEPTTAKIGEAFMKHYVFPFEFVSLILLVALIGAALIG
                     RSRTPGSGNGR"
     gene            18797..19102
                     /gene="nuoK"
                     /locus_tag="PLT86_02150"
     CDS             18797..19102
                     /gene="nuoK"
                     /locus_tag="PLT86_02150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT35447.1"
                     /translation="MVQLPHFLTVSALLFAAGVFAVVTRRNAVALLMGVELMLNAANL
                     NFAAFSRFSHNSLDGQIFAMLSIVTAAAEVAVALAIVLNIYRNIETIDADQAGDLRG"
     gene            19108..21204
                     /gene="nuoL"
                     /locus_tag="PLT86_02155"
     CDS             19108..21204
                     /gene="nuoL"
                     /locus_tag="PLT86_02155"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOT35448.1"
                     /translation="METTITTYALILALPLVAFVVQVFFGKRLPRGGDWLVIGAICID
                     LLLSLRVFFGALFFSSFAGTPFVWNLRGWLGDAGTGVLALSTGIMVDNVTAMMLVVVT
                     GCSTLIFLFSTGYMAGHRRYSLFFAYLSLFAFSMLLLVLGNNLIALFMGWELVGLSSY
                     LLIGFLFEEPVVAPDIVPASACKKAFITNRIGDTGFLLGILTLFSFTGKIAYGDVFEA
                     VRNGAFGDHATLLTVAGILLFCGAMAKSAQFPLHVWLPDAMAGPTPVSALIHAATMVA
                     AGVYMVARLYPLFTPDALTFIAYVGAFTALFGATIALVMNDIKRVLAYSTISQLGYMV
                     FGLGVGAYSAGLFHLMTHAFFKALLFLAAGSVIHAMHRAYHHAGLEWNPRSRENDAQD
                     MRNMGGLRKAMPVTFATTAVAVLAISGVPFTSGFLSKDMILGGAFAFGFVENPQHFLI
                     PVFGFAAAVLTAFYMARLLIMTFLGTNRMAERGQEALRESPWNMTVPLVLLAVLCGWF
                     WYNPDPTTIFAKGWVSSFLTPPASAVPQTLVAAPADAHHAAHVAAMLVSLSCAGAGFF
                     AAWMLGRRGAISLEHWRLSGSRVYYLLANKYYVDELYNRTVVAGMVQVSRILGWFDKV
                     LVDGVVNATGRFQTGLSELSGLVDRYGVDGLVNALAGFVRDTGNEVRQIQTGRIQNYV
                     VYATVGLIVLIVVTGM"
     gene            21218..22771
                     /locus_tag="PLT86_02160"
     CDS             21218..22771
                     /locus_tag="PLT86_02160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT35449.1"
                     /translation="MSNILSWLTFLPLVGMAAILVIPTRLRKSTGLIRYVALGTTLGC
                     FALAAWLFFAFDRSSSAMQFVENADWIPYFSIRYTLGVDGLSILMVLLTQIIGLVGVI
                     ASWNINKAVKGYFALYLLLQTGMTGVFCALDFVLFYVFWEVMLLPMYFLIGIWGGPRK
                     EYAAIKFFLYTLLGSVLMLLAILALYLNMQAPSFSITAMADPANFRADGWLVRNMPTW
                     FFGLTPKELVWLAFFVGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGILLKMGTYGI
                     LRVNFPILPAETVHFATFIAVIAVINIVYGALCAMAQKDLKSLVAYSSISHMGYVMLG
                     MVSWRNEAGLNGAVLQMFNHGTITAMLFLLVGVVYDRAHHRRIDGFGGLWAKAPVFSS
                     LFVVASMAALGLPGLSGFISELLVFMGAFASHRVFAVIASSGIVLGAAYLLWTIQRVF
                     MGPLNQAYAEKDAMPEISPREIASVVPLMAIVIVLGVYPAPALNLIGPTLGSLLQTMQ
                     SAAGAAILP"
     gene            22791..24269
                     /locus_tag="PLT86_02165"
     CDS             22791..24269
                     /locus_tag="PLT86_02165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOT35450.1"
                     /translation="MGSIQDHIISSVHAFAPEGVLAATLLLIIVADLLPATSRPAVSL
                     GLALGGLAVSAATVVRIPAGETLIFAGMLATDGFAATFKLVAAAVTAVVLVVSYRATD
                     VNREHFAEYCAMAIALCLGTYVMVAAANLLSMFLAFELVSMSSYILTAHMKEDRKASE
                     AALKYVVFSASSSAVMLYGMSLIYGLTGTLVLKAIMPALAGNTASLAQSVGVLFVLAG
                     LTYKIAAVPYHFWCPDVYEGAPAPIAAFLSVGPKAAGMAMLARFLNEALFLPPGSPIR
                     SGIDFGNWQTAIVLISAVTMTLGNLSALRQTNVKRLLAYSSIAHAGYMLMALAVIRPF
                     GVTAMTFYVVAYALMNLGAFLVVIALGRSMRSEELDSYAGLGGRAPVVAVLMAIFLFS
                     LAGIPPTMGFVGKLYLFWAVLEGKGLAWLAVVAALNTVIALYYYARILRQMFFLPARE
                     GAGEIRVEPIHYVVLFLLAIPTLALGVYWGPLWGAISAAGTP"
     gene            24290..24913
                     /locus_tag="PLT86_02170"
     CDS             24290..24913
                     /locus_tag="PLT86_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35451.1"
                     /translation="MGHTPLAISLLVCVLSLQSAAAEHVTRTRWFFSFGPSAIFLSNA
                     AHDDLAGVSAPAGTGRGGSAQLGFEVTSHFAMRLAVHSARHSVAGPGTGGTASAAVDF
                     VGIQKYGAIAPFLFGRVGKQAFVLSNARTTTSAPPRDHSWNGHLLGGGAGVRLYLTRR
                     LSVAPEASLCWVRYNKVMSTDGTGTQLPLDPISGTTGSVSLLLSIHW"
     gene            complement(24948..26015)
                     /gene="arsB"
                     /locus_tag="PLT86_02175"
     CDS             complement(24948..26015)
                     /gene="arsB"
                     /locus_tag="PLT86_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOT35452.1"
                     /translation="MSAVATKRLSFLDRFLTLWIFLGMAAGVAVGYFVPGTEGFIHRF
                     QVGTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNGKILGLSLVQNWIIGPVLMFVLA
                     ITFLSGYPEYMVGLIMIGLARCIAMVIVWNDLARGDTEYCAGLVAFNSIFQVLFYSVF
                     AWFFITVLPPLFGLRGAVVDVGIGQIAESVFIYLGIPFIAGALTRLVLVKSRGKEWYH
                     GQFIPRISPITLIALLFTIVVMFSLKGNLIVQIPLDVVRIAIPLLIYFVIMFLISFWI
                     GKKAGADYAKTATLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLVEVPVLIGL
                     VNVSLYLKGRWFESPQGATSA"
     gene            complement(26107..26340)
                     /locus_tag="PLT86_02180"
     CDS             complement(26107..26340)
                     /locus_tag="PLT86_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT35453.1"
                     /translation="MATYVYETIPGKPGEKPKRFEIQQRMSDEPLVTHPETGEPVRRV
                     ISGGLGTITSRGKQADGSRGERTLQCSCGGNCE"
     gene            complement(26469..27353)
                     /locus_tag="PLT86_02185"
     CDS             complement(26469..27353)
                     /locus_tag="PLT86_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT35454.1"
                     /translation="MNPFAVSLKFFVFILAELTALFLGISTLVGLMLQYVSDEKLRTW
                     LSRKGVLGNFLGAALGAITPFCSCSTIPMTVGLLRAGVPFGATMSFVLASPLLNPIIL
                     TMFLFLLGWKACVVYGAVTFVASMACGAALQGFGLASDVRNVRVSGVVHDQEKPETFR
                     GKLREAFAGAWGDFRGVLVYLVIGVAIGAAIYGYLPGDFVVGIAGPQNPFAIPVAAVI
                     GVPLYVRAETVIPIAVALTQKGMSLGAVIALIIGGAGMSIPEMSMLASIFKPRLVATF
                     VSVVLVTAVLAGVVFNVI"
     gene            complement(27448..>27663)
                     /gene="arsM"
                     /locus_tag="PLT86_02190"
     CDS             complement(27448..>27663)
                     /gene="arsM"
                     /locus_tag="PLT86_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="in Rhodopseudomonas palustris this protein confers
                     resistance to arsenite; catalyzes the formation of a
                     number of methylated intermediates from arsenite and SAM
                     producing trimethylarsine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOT35455.1"
                     /translation="ESMRTDDDLVCGCIGGAASVDEVRAWLENAGFQDIRVEIEEESR
                     ELIGRWVPGSGIENSVASAIIRGRKPE"
BASE COUNT         5442 a         8023 c         8179 g         6019 t
ORIGIN      
        1 gccatcggga tgccgggcaa aatctgggat cccaagatca tggcggtagg cgaaagaatc
       61 gcgactgcag cccggaacgc aggcaaaatg gcgggaacgg tatcggtgcc tgcgaaaatc
      121 cgcgcgctgg tggatatggg gtacaacttc ctcaacatcg gcagcgacgt gggagctctg
      181 accgagttct caaatcagcg cgtggatgca tttatggatt cgcttgctgg cctgaaggcc
      241 aacgggtgat atagtcctta gaccgcgcgt cttttcttga tggagaccga gaacatggac
      301 gtattcgcac cctccgcgtt ccttgatcca ccatccgaat tcagacccct tcagatcgtc
      361 cacgggtttg accgcttcct cgagcacccg ggtgatctga tcggcgagga ggggattgga
      421 cgccgtctgg atcagttgcg ccgagtcggg atcggcggcg ttgtgagcaa tgtgggagga
      481 ggatttggcg aatacctgct ggaggaacgc cagtgggagg tgtgggtttc cggcatgcag
      541 caggcacacg aacgtgggat ggtactctgg tggtacgatg aaaaagggta tccaagtggc
      601 actgcgggcg gaattgtcac gagagcgaat ccatcgctct gcgcagtcgg cctcgcgtgt
      661 tactcccttt cggcaaccgg accgggacgt ttccaattcg ttctgccggc gagctgtctg
      721 aagtttgaag tggcgtttgc cagcggagac ttcgacaggg tcggaatgga cggggtaatc
      781 ggtctcacaa gctccaccga cgagaacgga tgtctttact gggaagtgcc cgaaggcgac
      841 tggacggttc tctacctcgc gcaacgttac atgtatgaag gcacccacgc agcggccaat
      901 gtctgcgagt tcaagcatta cgttaatctg ctggagccgg aggcgacgcg ccgctttctg
      961 gcggtcaccc acgatgagta tttccggcgt actccgcccg gtctgtggaa gaatgttcgc
     1021 gcggttttca ctgacgaacc gtcgttgatg acggggtacg caggtccgct tccgcaacgg
     1081 ttccaaggaa atgtaccggt aatcgacaag cctctctttc gagaccgtcc ggtggcggtt
     1141 ccctggtctc aaggtttggt tgccgcgttc actgagcgca aagggtacga tccgaagcct
     1201 cgtctctggt gtcttttctg tggtgaacat gaagacgcgc gggcgttccg gcaggatttc
     1261 tacgaaatcg taaccgaact ctacaccaac ggattctacc gccaggttcg gaactggtgt
     1321 gaccggcaca acattgcatt ttccgggcat gtaatggcag aggaatggct cggaggccac
     1381 gtggagtacc acggaagcct gttcgcgccc gtccgcgaaa tgagtctccc gggaatcgac
     1441 atgctggatt cagaccccgt gtcgatcctc gagggacacg ggttcatgac tgccaagcag
     1501 gtcgcttcga ttgcccatct caccggtgcg cagcaggtgc attcggaatg ttctgactgg
     1561 tcccagcgca acaacggtcg tggcgccacc ctggaacagc ggtacgggca gggaggcttg
     1621 ctctacgtgc tcggggtgaa ccaggttacc gcgtactgga gctgggacga catcggcgat
     1681 gaggcgtacc ggacgtacaa tgactacatg ggccgccttg gcatgttgct gcgcggtggt
     1741 gtccatcgct gcgacgtggc cgtgctgtac ccgatccgaa cggcgtggct ggatttcctg
     1801 ccacatgcat cctacgaggc tgataacgcg ggtcagaagg tggtgtccac tgcgggcgcg
     1861 cgtgagcggt tcaacgccgt cggagcggca tacgcggata ccgtgcggga cctgctgcgc
     1921 agtcagatcg accttgacat cattgacgag caggcgctca ccgaaggcat catcggtgca
     1981 ggagcgttgc atgttgcagg tgaatcctat cgcgtaatag tcttgccgca atgtgaagcc
     2041 atctcacttt ccgcagcgga ggctcttgcg gcgttcgggc ggtcgggagg gctggtagta
     2101 agcatagggg caccgccacg actcgtggaa tccgcccgcg cgacagcgaa ggttcacgag
     2161 ggtatggagc agctgttcgg tgccaggggg aacggacgaa ttctcaagaa ggaagaggtg
     2221 gttccttacc tgcggtccgc gcgctgcgca actctggagc tggcagaggc gaacccttcc
     2281 attctgtgca cagagagatc acgtgatggc cgcgggttgt gcatgatcgt gaacgcgagc
     2341 ggagagcccc aactcatcag gccgcgttcc cgactgaggg agcctcttga cctgtatcgg
     2401 ccgctggacg ggtctgtcgc gccattccgt gcgggcgctg aactccatct cgggagtttt
     2461 gaaagcgttt ttcttgttgc acacgaacct tcaccaaccg tgtgaaagga agacactggt
     2521 cagccagcat acttgtaccg ggtgatgatt tcctcctggg tggcgggtga gagcgtgcca
     2581 aagcgggcgg accaccctgc aacgcggact atgaggtccc tgtattcgtc cgggttctgc
     2641 tgggctttgc ggagggtttc cacggagatg acgttgccct gaaaaatgtg accgccccgg
     2701 tccacaaagg ttttgagcag cggctttacc acggatggcg ccgcccacga aggatccagg
     2761 tcccacatca tggcgccccc tccccccgct ttgtcgagag agagactggt ggccgaacga
     2821 attgctcccg tgatcccctt gaccgcggaa cccgactgcg gggagaggcc gtgtgccagt
     2881 gggcggccgg cgttgcggcc atccggcgta gcccccactt gtctgccaaa atccaccacc
     2941 catacaaagc caaggatgat cggtacaagc gttccgccgt ggcgcgtcgt atggctggcc
     3001 agaagatcgg caaacgtaca aaagacccgg tcggtcatcg catctgcggc ctcgtcaccg
     3061 gcaccgtact tcggcagaga acggaggtat gcctgaagtg cttcgaaccc cacaaagtcg
     3121 gtgcgcaatg cgttcaacaa ttccgccgct gtcacgcggc ggtccagaaa aacggctcgc
     3181 tgaatggcgt aaaggctgtc gcccacgttg ggaatgccga cgccgctgcc accatagttc
     3241 gtataccgag cgcccccatc gttgattgag cggccctttt cgaggcagtc atgagtcatg
     3301 ctggaaagga ggaaggaggg ccgcagacgc gcatattcct ccagatagat atcgagacgg
     3361 cgggcgagta tccccagttc acgtgcaagc tccgcctcga aatctgtgta aagctcctca
     3421 aaggatgtgt agtcagccag cgttttcggg tggtcgacga cacgttcccc ggtctgcatc
     3481 aggcagccac cgttgatgac gagctcaaat gtgcgggcga cgttatgcgc aaacgagaag
     3541 agcaggtcgc agttgcaccc ttgcggtgat acttccatgc atccgccggc agtccagtga
     3601 cgtgcgtcct ccacggcgac tttgccgctt ctgtgaagcg cttctatgac ggcatcgtca
     3661 ccatacacct gcgctcggtt acctgcgcgg agcaggtaac ggacggtgag gtcaacaaag
     3721 tcgtcgggcg cgtcgtccca ggcgtacgta gacggagggg tgcgtctgcg gcaactcgga
     3781 gataatctcg atgatcatgg aggaaagcgg attgatggca aggcttccgt ccggtttcct
     3841 gcctcccacg ggcaatgctt ccacccatcc gtccgccgga gcaatccgtt cgtgcagctt
     3901 gatgagcaac tcggtcacaa gctctctggc ttcgtcgagg gctattctgc ctgacgcgag
     3961 gtcgcgctca aggaagggcc acaggtaata gtcgagccgc cccggtccat tccctccgaa
     4021 aggattctca tacatgacgg cgaggtgttg aaaccagaac gtctgcacgg cttcgcggaa
     4081 cgttgttgac gggttgcgtg gcacccgacg gcagacgtcc gccatttcct ggagttcctt
     4141 gcggcggacc gggtcggttt cttctctgat cagatcttcg atggtgctga catggagttc
     4201 gacccatgaa agcaacccct gcaggacaat ggccacgcag tgatagaact cgcgggatcg
     4261 tggggctgtc gtctgtctct ggccccgttc gcacagaccc agcatgcctt cgatcccgag
     4321 gtccagcatc cgcccgaaat cccagcatat gtgcccggga taccctccct gcgtgaagta
     4381 tttcccgtag tactcgccgt ccgggaactc ttccgcgacg cgacgctccg cagattgccc
     4441 gatggagaaa tcaacccagc ccggatgctc tgtgctgcca ctgagttccg gacacgcgga
     4501 accagcacac gcctgataga tctgaccggg aatgcgtgag agcgggtgaa tcaggaccgg
     4561 ttcattcacc agcgcgtacg cgagactgcg cgcctggcgt tcaggaaggg aaaagtcggc
     4621 atagaggtcg aaaaaggcgt acgagtagtt cttttctttc gggctgtggt gggaggcaaa
     4681 accgcgtgcg taggcctggt ccctgaatgc cgccaatcgg tcctgcataa ggaccctttc
     4741 ctctcgttgg ggatgtacac gcgggcaacg catcagacgg gccgcgctcc gaacgaagtc
     4801 aatttaccga gtcacccgtc tccacgtcaa acgcaaggca gtgtaccgaa ggcatcggga
     4861 agaccgactg gcgccgagca cggccttgca ctaaggaaaa agcagtgact ttgcgctgat
     4921 cataagtcac tgcttacatt agatatgagc ccggcgggaa tcgaacccac gaccaacaga
     4981 ttaaaagtct gctgctctac cgtctgagct acgggccctg aaacaggcaa gatttttaaa
     5041 tttactcatt tgcgcgcgga tgtgtcaact acgctcagaa cgtgcggcaa ggttggcttc
     5101 ccgatgcgcg ggtcgggatc gtatagatga ttcgccgcat gtacgactgg gttctgagtt
     5161 gggctgagca tcccgcaggc gttgttgcac tggcggttct cgccttctct gaatcttcat
     5221 tcttcccgat tcccccggat gtgcttctta tcgctcttgc catgggcaaa ccgaaacggg
     5281 cattctggtt tgctgccgtc tgtacgatgg gctccgtcgc aggtgccatg ttcgggtatc
     5341 tgatcggcag tgtcttctgg aatgcggtga gcccttattt cttttcctac gttcccggat
     5401 tcacgccgcg tcagttcgag atcgtcagag ggatgtacga gcagtacaac ttctggattg
     5461 tcttcactgc tgcattcact ccgatcccct acaaggtcat caccgtcacg gccggtgtat
     5521 tcggcatcaa tttcccgctc ttcctcgttg ggtccatcat cgggcgtgcg ggacgtttct
     5581 tccttgtcgg ggcaatgatc tggagattcg ggcccccgat caaaacgttc atcgaccggt
     5641 atttcaacgc acttgccgta gcttttacgg ttttgctcgt gggcgggttc gtcgccatca
     5701 agtacgcgtt ctgagcactt ggatggggtt agtcgacagg ggtgactatg agtggttttc
     5761 gcaagaacag cgtcgcgtat tcaccgaaac aatgggagat gcggtggcat ccgatccgga
     5821 aagagtgggt gggatttgcc gcgcaccgca atacacgccc ctggcagggg gaacaggcgc
     5881 cgcagccgcc tccgccgccg cctttcgatc cgacgtgcta cctctgcccc ggcaacacac
     5941 ggaaccgggg ggaaagaaac cccgattatt cgggaacatt cgttttcgac aacgattttc
     6001 ctccttttgg gcccgatgcg ccggaaacca ctaccgagca cggcctgtac aaggcgatgc
     6061 cggcgtacgg acgttgccgg gttgtctgtt ttcacccccg gcacgatctc acccttgccg
     6121 aaatgaatcg agaagatatt cgccgcgtgg ttgagacctg gcagactgaa cacagaatgc
     6181 tcgcaaagga tagccgtatc aaccacgtgc tgattttcga gaacaaaggg agcgtcgtgg
     6241 gtgtgagcaa tccccacccg cacgggcaga tttacgcaac ggagttcgtg cctccggtga
     6301 ccgtcacaga gctcgcatca tcccgggagt tctacgcaga gaaacgccgc acgcttgttg
     6361 gagccattct ggaacaggaa ctgcgggatg aggttcgcgt cattgcgcag aacgatacgt
     6421 cggttgcttt catcccgtat tttgcgcggt acgcgcacga aacatacatc gtaccgcgcc
     6481 gccccatgcc gtcagttgcg gatctcaccc cggacgaagc ggacgacttc acagagatct
     6541 tacatacgat gctcgtgaag ttcgacaacc tgtacggtat ttctttcccc tatgtgatgg
     6601 cgttccatca ggctccaacg gatggcgccg cccacccgga ataccatttt cacgtcgaat
     6661 tccacccgcc actccgctac ccgggagtgc ttaagtacct cgccggcgcg gaaaccggag
     6721 cgggtgtttt tcttaacgac acgtcacccg aggagaaagc gaaggaattc cgcgcggccg
     6781 gtaacatcca ttacaaaacg accggcggcc agaagacagc ctgatgtttc cgcacaaaag
     6841 aaaggcctgg aagtatttac cccaggcctt tcgaacaatc gatcggcaaa cgattacttg
     6901 tctttgccgg gaaggttgaa gaactcgcgc atgttttcgt acaggacttt ccggtcctcg
     6961 ggatcagcga gattgaggcc atattcgttg atcaacatca cctctctcct agtccactcg
     7021 tcccacgctt cctgactcac gttctcccat atctcctgac cgattttccc ccgcatcggc
     7081 ggtttttcca tctgcggcac gaccttgccg gttcgggagc aggttatcat ctttgccatg
     7141 ggaactcctt atcatgtatg gcatggtctg gctactgaac gaatccaaag catagcaacg
     7201 cgcgccacga atatcaatgt gcgtcgagag ctttctttcg cgaggcagca gttgtgaata
     7261 ccgaacatga gtgtctcatc tggtgggagg aagtccacaa gagctttgga gcgaaccacg
     7321 tcctgcgtgg ggtcacgctc caggtgtgcc agggcgaaac cctggtcatc atcgggcgct
     7381 caggatgtgg caaaagcgtg ttgctcaaga cactggtggg actcatctcc ccggattccg
     7441 gcagaatctg ggtgaaagat cgccagattg tcggtgatcc atccgcgatg gcggaagccc
     7501 agaggctttg cgggatggtg ttccagggag cggcgttgtt cgattctctt tcagtcgccg
     7561 aaaacctggc attgccctac tgggagaaca ccgacctcag caagggggaa atcgaggaac
     7621 ggatacggtc attgctcgca cttgtggggc tgcgcgatgt agaacaactc atgccggcag
     7681 agttatcagg cggcatgcgc aaacgcgtaa gccttgcacg ggcgctggct gacgaacccg
     7741 agatcatgct ctacgacgaa ccaaccacag gtctcgatcc tatcatggca gatgctatca
     7801 acgagcttat caggtcgacg caggataagg tcaaattgac gtcgatagtg gtaacgcatg
     7861 acctgagtac cgtgcggaaa gttgcgaacc atgttgcaat gcttcatgag gggcgcatcg
     7921 tattccacgg gactccggac gagttgttcg cgtcagccga tcccgtggta acacagttca
     7981 tccaggggcg cgcggacgcg cgtgtcgagg tcccgcgccc cgaactgcgc gagcacgaaa
     8041 tcgatgagta agccgaaccg ggaagagcgc cgatcggcct cgtcgagttg ggtttcctgg
     8101 ctcgtggaaa tggcgcgtgc ggagaggctc tggtttggcg ggaccgtgtt gttttccgtt
     8161 atgggcgctc tcacgggagc gctcgccacg tggctgatgg ttcccctggc ggctgttctg
     8221 ttccccgaaa gcactggagc ggacgcgatt cacaacccct caagagggct gtatcccgtt
     8281 tctgcaggcg cctggttgat gccgacaatc gccagcatca cacccctctc agtcgctctc
     8341 tcattcgttg ttgtcttcac cgtgaagaat ctctgcgagt tcggctcgcg cgtggggtgc
     8401 gacgccctgg tcgcaaaggt tgaattccgg ctgcgcaatt ccctgtggcg ccagtatctg
     8461 gcatgtgggc gcgcgtcagg gcgttctttt tccggggcgc gattcatcca cgaactgctt
     8521 gccggcgcgg gggagccggc cgccgcggtc ggagcaattc cggtgcgttg gatcggtgac
     8581 ccggtcgctg cggtggccct tctcggcacg atggcgataa tcagccccca tcttacgtcc
     8641 gcgatgttgc tgcttcttcc acccgcgtgg tgggcaaccc ggaagggctt ccgccacatt
     8701 ggcaaccgaa cggagacaag gcttgccgcg cggggacgac ttggagcgcg tttgagtgaa
     8761 ctcgcctcgt tccgcccggt gctggcaagc cacgccgcga tggattgggc aatgatcaac
     8821 accggccggg acgagcaatc ggtaatccgg gcagcccttt catggtcccg cgttgctcgt
     8881 ttgatcccgc tgactgccga gaccctcggc gcgttcgtcg gcgccgcggt gattctcgtc
     8941 gttgcaaaag gcatacgtgc gggaacgctt tcgggcggag agttcttgac ctccctcact
     9001 gcgtttttcc ttctccttcc agtggcaaag cggctggcag cgttaagtgg agacatgcga
     9061 acatcccgtt ctgcgtggga aaggctcgct tccttgcttc ctgctgacgc aagagccgaa
     9121 gtggccgtac ccccttcgga aacgttccgt gcacccgcga ttactctccg cacacttcgg
     9181 gcaagcattc ccggcggcaa ccgggtgctc gacatcgggt acgcagaatt cccccccgga
     9241 gaggtgactg ccatcgttgg tccgaccggt tccgggaaga gcctgctttt ggaaatcatc
     9301 gcggggtttg gtgttccaga cgaaggaacg gtatgctgga acgaacggcc gatctcctca
     9361 ccacatgcgg ttccagaaag gctgccgttc gggtacgttc cccaggatcg gtggacaatc
     9421 tccggcacgg tcggcgaaaa tgtgacgctc gggagaccgg tttcggaaac cgccctgaca
     9481 acgactctcc aggctctggg gttggatctg gacgttcaga ccgaactggg gcaggacggc
     9541 gcgccgcttt ccggtggcga gaggcagcgc ctggcgctgg caagagcgtt gctcggtgag
     9601 cctccgctgc tgcttctcga cgaacccacc tcagcgctcg acgcggcgag cgaggaacgc
     9661 gtcatcgctc ttctacgctc cctgaagggc agaaccacgg ttctggtggc aacgcacagc
     9721 accgccgtca tcagtgccgc ggactgggtg tatcatttgc ctttccaatt tgcgacaacc
     9781 gctctgcaca aggaactggt gacataatgc gtgtcaagat gctggcggcc ggatgcgtgt
     9841 tcgctctttc gtgctgcact tcgccccgtg cggatgtgac ctcccgctac gctgcgcaat
     9901 ttgaggaagc cctggccagc atggcgatgt cgcccgcaga cgtgacattc cgaacagatc
     9961 acgtcgaacg ggattccttc cggctccgac gaacggacgt attccttctt caccccctct
    10021 ccttcgccga ctcgcttgca tcgttctccg caagagcggg cgagttcgga ggtccctcgc
    10081 cgtatgtcca cctcgcggcg gattggcttg atgatcccct ccccccgttg tgcgagcctg
    10141 ctgcgcgagt gcttgtcaga gatacaacac gatgcgcacg tgcgttcaat cgcctggcag
    10201 gtgcaatcga acatgcggcg cgcattgttc aagagagcca gagcgttttc tctccggcgg
    10261 aacgggagtc gctctcaaca tgggtagaca gagtgctgtc ggagggagac ccggacgtat
    10321 caggagaaca agtagacctg ttcgcgtgga acgctgaaga gctggcggaa ttgcagtgct
    10381 cgcgggctaa tcttgaactc gcaaaaagat ggagtggacg ggaaattctc cgcgccgcga
    10441 gttgcgtcgc gtcggcagcc tgggagttgc gtgaagcgct catggaagcg aagaaccggg
    10501 tgcccatgcc gttgcctgtt aaaacatggc acaccacctg cggacgtatg accgtgggaa
    10561 gcgacgccca agacaactac acaggagatt tcgcgctcat cgttgatcag agcggagacg
    10621 acacgtaccg cgtgcggcct cccggttctg gcggcgggca ggtgcaagtt gtcatcgaca
    10681 tttccggcaa cgacgtctac ttcggaagca tcgcgggaag cacgttcgga atcgcgatct
    10741 ccctcgacgt ctcaggcaat gacacctaca taggcgaggc ctggacgcaa ggctccgccc
    10801 gttttggcgc ggcgatcctg tgggatgaac gaggaaacga tacctattac gccaaccgag
    10861 cggtgcaggg aacgggtatg ttcggactcg ggctgcttgt ggacgtggac gggaatgacc
    10921 tctatcgatc gggcacctac ggccagggct tcggggccac cggcggaatc ggcattctgg
    10981 acgatcgaaa gggaaacgat tcgtactacg cgggaggcgc gactactgac gtgttgcgct
    11041 ttgatgacca ctacctcacc atggcacagg gggtcggatc gggcattcgt cccgttgggt
    11101 ccggcgggtt cggttttctg attgatcgga aggggaatga cgtctacaat gccgacgtgt
    11161 acggacaagg cgtgggttac tggctgggcg gtggcgggct tcttgacggt gaagggcatg
    11221 accgctacgt ggcgcatcag tacgcacagg gtgccggaat ccacctttcc tcgggcgctc
    11281 ttctcgattt ccgcggagat gatgtctatg tgatcaacgg cgttggccag ggatgtgggc
    11341 acgacctcag cgtgggaatc ctcttcgacg aaactggcga cgacgcattc accgtggaag
    11401 gcctcgctct gggtgccggg aacgcaaacg ggatcagcgt gttcgcgaac gtgagcggtc
    11461 gtgacgcgta catcgcccgg cgtgaggacg tcatgggata cagtgataga cggcgcgaat
    11521 atggtatgat cggagtcatg ctcgacctcg ggggcgaaga ccgctatgca gcacccttgg
    11581 gcgccaatga caggtggtgg caccattcaa cgttcggggc cggagtcgat ctgggctccg
    11641 tttctccctc ttccgatacc gcgaccgcgg ctgatctgtc actcaccgag ggaggacacc
    11701 gccagaagat tgcggcgact gctgagtctt tgtttgtcca ggcttcgaac ccgttttccg
    11761 cgctccagta ccttgttgaa ccagcagaaa accgacttgc cgacatggtg ggggaatctg
    11821 tgacggcgtt ctgggcaggc aaactcgcct cagaaagcgc gagggaacgc tgggccctcg
    11881 tgcgtatcca tcagaagttg tttgcccgag gggacctcag cagcgtcccg atgttgatcg
    11941 attcgctcga atccccttcg ggcgcggcac gtcgtatggc cgcacatctc ctaggctttt
    12001 cgaagtatcc agatgcggca ctgccgctga ccggtctact gcggcacccc gattggaaaa
    12061 cacggcaggt cgcagccgaa agcctgtgga ggctgaaaga cacccgttcc gagcctgctc
    12121 tggtctctct tctggaggat tcagttgcgc tggtccgaca cgccgcggct ctagctcttg
    12181 gaacatgcgg gacatcacga tcggacacgg cgcttgtacg acgcctgtcg gatgagagcc
    12241 agatcgtgcg ctacgccgcg gagcaggcgc tcgggccaag ggcaactgcg cggccgcttt
    12301 tgcttcagat tgcgggatcg caaggtacct tcgcggcgat gcatgccctg cgcgcgcttc
    12361 gcgtcgacac gtcggacacg tcgtacgctc cggtgctacg ttcgatcatt cgatcggacg
    12421 gacactgggc ggttcgggca gaggcggcca acagcatttc cgcgttgcgc atgcagagtc
    12481 tgtccagcga tcttcaggag gtgaggatgg acacgcacca tccctttctc gcccagagga
    12541 tcgaagaggc aatcaccacg ctgaacggcg cgaaccggag aaacgaatga acgatgactt
    12601 tgtgctgaaa accggcgaaa atcttgaatg ccgtgtgata gaccgtcgga atactgatct
    12661 tgtcccagcg ctccggaccc ttctcgcgca caaaggagat atctggcagt tccacctgga
    12721 agaggagttt gccggccgga tcaaggatct ggaaacgcgg ttttacgtgg ggctgattgg
    12781 ctcggatctc gtcggcaaca tcatgactgt cgagttcaag ggacttggaa tcatggggca
    12841 tgtgtacact ccgccagagc atcgtcgcaa gggaatctgc gatgttctga tggactttca
    12901 catggccgat ttccgggagc gaaaagggaa cgcgttgtac ctcaatacgg ggttcgagtc
    12961 ggccccgttc ttcatttacc agaagcacgg gtacaggccg attcctgagg cccccgggtc
    13021 aatgtggtgg tcacccgatt catggagtcc ggacacattg tgggacgatc tcgagcgcct
    13081 caaaatcgcc gaacctgcgt ggaggcactg gccgtcggcc aacgtgttta tgctgcagac
    13141 ctgcctcccc gccgtccgca acgtgacgta cgggaaattc gggtgcgcaa acgccgaagc
    13201 cacattcctc cacgtgttgt cggacctgcg gaaccccgaa aagcgcgtcc aggcgcgggt
    13261 gctggaaacg cgcgatggcc atatggccgg tctggctaca ctggcgccgg aacggcggtg
    13321 gggttccctc gggaccacgt acgtgttcga cctatgcgtg caccccaacg cggaagatgt
    13381 ccctgaacag cttgcggagg atttccagtg gcctgcggcc cacgtactgg cgtacgccgc
    13441 ggacgatgac accgcagccg tgaaccgata tgcgcagatg ggattccgcc cccacagtca
    13501 catcgaaagg ttcttcggca ctaccggact cctcatcctc agccgcgaat gagcgcacca
    13561 gcgggtggct cgcagtccca acgcgagcag gtggaaaccc gggaatcctt gcgtcagcga
    13621 tacgccttcc ctactttctt gcatacagaa gttgcgtttg tggcaccgtt tgcacattcc
    13681 ctcagcgcga tccgcatctc cctggagtaa tcatgtacct ccatttcggt gctgttcttg
    13741 cttttctgct gttcagtgcg gtgttcgtgg caatcaccta tggcatcagc gcactttttc
    13801 gccccgcgaa cccgtccgcg gaaaagctcg ctacgtacga atgcggcgaa atccctttcg
    13861 ggggttcgtg gatccagttc aatctccggt actatcttat cgccctggtg ttcctcatct
    13921 tcgaggtgga ggtggtgttt ctgtacccgt gggcggtcgt gttgcggacc ataggtgcat
    13981 tcgcgttctg ggagatgatc gttttcctcg ccatccttct tgtcggcttc gcgtacgtct
    14041 ggcggaaagg cgacctttcc tggttcactc cctctggttc aggacacacg gacaccacgt
    14101 tttcggggaa atgaatactg tgacacgttc tgcaggctgt catcggatga tatgtgtgga
    14161 aaccgagcag ttcgaaaagg tgtaagggat ctcccgatgg gcatgctgga agagagattc
    14221 gatccgaaca tcgtcaccac gaccgtggac gccttcctgg actgggcacg caagtcttcg
    14281 ttgtggccga tgggattcgg gttggcgtgc tgcgcgatcg agatgatggc taccagtatg
    14341 agccggtatg acctcgatcg gttcggagca tcagtgtttc ggtcctcacc caggcagtgc
    14401 gatctcatga tcgtgtcggg aacggtgacc ctgaaaatgg cctcgcgagt caagcgtctt
    14461 tacgaccaga tggccgagcc cagatacgtg atttccatgg gcagttgcgc gaacaccggc
    14521 ggaccatact gggaacacgg gtaccatgtc ctgaaaggcg tggatcgcat cattcccgtg
    14581 gatgtgtaca ttgcggggtg tcctcctcgc cccgaggcat tgattgatgg cgtgctgcag
    14641 ctccagaaaa aaatcacgga acaacgcgtg gtcaggaagg cggtgtgagg cagacttgga
    14701 cgcgttggca atcgcaacga tggtgaagga cgtctttggc ggcgcggttc ttgccgttga
    14761 ccagccggag gtcgagcctg tagcagtgat cgaacccgcg aacgtggcgg aggttgctcg
    14821 tttcctgagg gaaacaccgg aactcgcatt cgactatctg atgtgcctca gcggcttcga
    14881 tacgatgccg actgaggagg atcatccagc ccggaacacg ccggaagagg ataccggagc
    14941 accctccacc gaaacggcgg cacctgccct tggcgtggcg taccatctct tttcctattc
    15001 tcacagccac cggttcgcgt tcaaagcgca cacgccgcgc gaaaatcccc acattcccag
    15061 tgttgcggag atatggcctg ctgccgactg gcacgaacgt gaggcgttcg accttttcgg
    15121 aatcatcttc gatgggcatc cagacctgcg cagaatcctg cttcccgagg actgggtcgg
    15181 gcatcctctg cgaaaagact acgcagcgca ggaaagctac aggatcgggg acgcctgcgc
    15241 gcaaattgcg cggggttggt aacccctgga aaacagccgg aagactatgc cgctcaaaac
    15301 agaggaaatg cttctcaatg tggggcctca gcacccgtcg acgcacgggg tgcttcggct
    15361 ggaggtaacc accgacggcg agcttgtcac cagtgctgag ccgaccatcg ggtacctcca
    15421 ccgctgtttt gagaaatacg ccgagacact cacctaccaa caaataatcc cctacactga
    15481 ccggcttgac tacctctcgt cgatgaacaa caatttcggt tttgtcgttg ccgtagaaaa
    15541 acttatgggg attcacgtgt gcgaacgggc ggagcggatt cgggtgatca tggcagagct
    15601 caaccgcatc gccagccact tgatgttcat cggaagcttc agcaatgatc ttggagccat
    15661 gacgcctttt ctgtggtgct tccgcgacag ggaggagatc ctcaagatct tcgaggaagt
    15721 atcgggcgcg cggcttcttt acaactatta tcggataggc ggcctcgcag ccgacctgac
    15781 ccccggcatc gaagagaaga tttactcgtt tctcaaccag ttcgaagcgc acatgctcga
    15841 gctggacgac cttttcacgc gcaacgctat cgtgatagcc cgtctcaagg gtgtggggat
    15901 gattccccgc gagatggcca tcgcgtacgg gctttccggc ccgatgctcc gcgccagcgg
    15961 ggtagattgg gattgccggc gtgacgaacc gtattcgatt tacgcgtcgc ttcgttttga
    16021 agtcccggta ggaggcgaaa aactcggcgc tttgggcgat tgttacgcgc ggtatctggt
    16081 ccgccgggcc gagatcgacc aatctgtatc actcgtacgg caatgccttg ccgatattcc
    16141 gccgggagac gtcaaagaac aggtgaaacg aaacgtccgc ccacctgtcg gcgaggttta
    16201 cgtgagaacc gaaaatccgc ggggagagat cggtttctac ctgcgaagtg acggatcgac
    16261 gaaaccctcg cgtgtgaaag ttcgtgcccc gagcttctgc aacatgagtg tgttcagccg
    16321 catttccaaa gacatcctgg tagcggacat gatcgccatt ctcgggagcg ttgatatcgt
    16381 tctcggcgaa atcgacagat gacgcacaac ggaaaacact gatgcaggag atgtacgaaa
    16441 cactcctcgc cgcgcttggt gccgagaggg tcatcccttt ttcggtcgga tttgccgtgc
    16501 tggcggtcgt tttcgcattg ttcgcggcga tcatgggtgc tgtagtggca ctcataatgg
    16561 ttctcgccga acggaagatt tctgcggata tccaggaacg aataggaccg aatcgcgtgg
    16621 gcccgatggg tttcttccag acggtagccg atgcggtaaa gcttctcacg aaggaaaacg
    16681 tccttccacg aaaagccgac agtatcctcc actattgcgc accaacgctt gtgttcacag
    16741 gcgcgcttct accggtggcc gccatcgcgt ggagcaaccg cattgtggcc gctgacctga
    16801 acatcggcgt cctgtatgtc gttgggacag cgagcttggg ggtgattggc aggctcatgg
    16861 ccgggtgggc ctcaaacaac aaatggtcgt tgctcggcgg gatgcgcgcc gccggacaaa
    16921 tgatctcgta cgagataccc aacagtatcg ccatcctttc ggtgattgtc ttggcgggaa
    16981 cactgaatct gcaggaactc tcagaggctc agatgggcgg gattcaccag tggtttgtgt
    17041 tccgctcgcc gttcgccttc gtcgcctgtc tcatttactt attgagttca ctggccgaag
    17101 tgaaccgtac accgtttgat ctgccggaag cggaatcgga gcttgtatcc ggttaccata
    17161 ccgaatattc cggaattcgc tgggcgattt tcatgatgtc ggagtacatc ggtatggtgg
    17221 ttgtgaatgc tgttgccgtc gctttgttct tcggcggatg gaacggcgga ttgcggggag
    17281 aacccctgct gggcggctgg atctggtatc tgctcaaaat cgccgttctg atgtactgcc
    17341 aagtctggat aaggtttgtg ctgccgagac ttcgcataga ccagctcatg tcactgtgct
    17401 ggaaaaacct cgtccctctc ggtttcctcg cgttgctggg agcatcggtg caggcactta
    17461 tgggccccac gggccagcgc gtgatgatgg ttgctgcgtg gctcgtgttt ggcggatttg
    17521 taacactcct gttcgtggcg gtccgcaaga cacgacacgt tcctgcgcag ggtggatttt
    17581 ccggatgagg gccttcttcg ggtactggcg aggcattgcc tccggtttca tgacgacact
    17641 ggtcggcatg gtggcagcca tcaggcacgt cggccggaag gaatacacgc tggaataccc
    17701 agaacgtcgc gacgtacttc ctccccgggc ccggatgtcg ctgttcatga acgcggacga
    17761 atgcatcgcg tgcgctcagt gtgcccgcgc gtgccctgta aactgcataa cggtagcttc
    17821 ggtcaaacgt cccaaggatg aggaaccgcc cgtcacggtt gtcggacaac gtaaaaaggt
    17881 gctggtgctc acgggataca aaattgacat gggaaggtgt tgtttctgcg gtctgtgcgt
    17941 cgaaccctgc ccgacgcaca gcctttacat gactccttat ttcgaaatgg cagttgtgca
    18001 aaaggccgcg gaaccggacg aaatcctcca cgagggagaa agcttcgcgg ccgcagatgc
    18061 gcgacgaact cggtcggaga tacaggtcca gcatccgagg tttggccgta cggggctcgt
    18121 gtacgatttt ctggcgatca ctgacgatct gcggcgtcga ggcgaaacgg tacctgtgaa
    18181 tccgcttttc cccgaagagc acgctcagtt ggtacaaaac cgggtccggc cgccaaacca
    18241 ggacgccgct ggtaccacgg atccccctgg aggccgcaat tgaacgcgta cgacctcgcc
    18301 ttctacttgc tcgcagcgat ggcgctggtt ggcgcggcca ttaccgtttt gagccgtcgc
    18361 cttgtgtacg ccgcattcag ccttttgctc gcctttctcg gcgttgcagg tctctacgca
    18421 cttctggcgg ccgatttcct tgcaatcgca caggtggtag tctacgtggg cggaattctc
    18481 gtgttgcttc tcttcggggt tatgctgacg catcgaattt acgacacgac cctgaagagc
    18541 gtgcgcgttc agccgctcct cggcggcctc gtcgccgcag ggatagccat cctgcttatc
    18601 agtgtggcat ggaatacgcc ctggaacaca acacacgaga tcgccgcgga accgacgacg
    18661 gcaaaaatcg gcgaagcgtt catgaagcat tacgtgttcc cgttcgagtt cgtgtcgctg
    18721 atcctgctcg tagccctcat cggtgctgct ctgatcggac gtagccgcac acccggttca
    18781 ggcaacggac gttgatatgg tacaacttcc tcattttctc accgttagcg cgctcctttt
    18841 tgcggcgggc gtgttcgcag tagtgacgcg acgaaacgca gtcgcgctcc ttatgggtgt
    18901 ggaattgatg ctgaacgccg cgaacttgaa tttcgccgca ttctcgcggt tcagccacaa
    18961 ttcgctggac ggacagattt ttgcgatgct gagcatcgtt acggctgcgg cagaggttgc
    19021 agtagcactt gcgatcgtac tgaacattta ccgcaacatc gaaaccattg acgccgacca
    19081 ggcgggcgat ctcaggggat aaggctcgtg gaaacaacca tcacgacgta tgcgctcatc
    19141 ctcgcgctcc cgcttgtcgc gtttgttgtg caggtgtttt tcggaaaaag gctccctcga
    19201 gggggagatt ggttggtaat cggcgcgatc tgtatcgacc tgttgctgtc cctgcgagtc
    19261 ttctttggag cgctgttctt ctcctcattc gccggcacac catttgtgtg gaacctgcgc
    19321 ggctggcttg gcgacgcggg cacgggggtt ctcgcgttgt caaccggcat catggtggat
    19381 aatgtcaccg cgatgatgct tgtggttgtg acgggttgca gcaccctcat tttcctgttc
    19441 agcacagggt acatggcggg tcaccgtcgc tacagcctgt tcttcgcata tctgtccctg
    19501 ttcgcgtttt ccatgctcct gctggttttg ggcaacaatc tgatcgcgct gttcatgggg
    19561 tgggaactcg tcggcttgag ttcatacctg ctcataggct tcctgttcga ggagccggtg
    19621 gtagcccccg atatcgttcc agcctccgcc tgcaaaaaag cattcatcac gaaccgcatc
    19681 ggtgatacag gttttctgct cggaatcctg accctgttct ctttcaccgg aaaaatcgcg
    19741 tacggtgacg tcttcgaagc ggttcggaac ggcgccttcg gtgaccatgc aacccttctg
    19801 actgtcgccg gaattctctt gttctgcggc gcgatggcga aatccgccca gttcccgttg
    19861 catgtgtggc ttcccgacgc gatggcaggc ccgacgccgg tgagtgctct gattcacgcc
    19921 gcaacgatgg tagcggcggg tgtatacatg gttgcccggt tgtacccgct tttcaccccg
    19981 gatgccctga ccttcatcgc atacgtaggc gcgttcactg cgttgttcgg cgccaccatc
    20041 gcgctggtga tgaacgacat caagcgggtg ctcgcctaca gcaccatcag ccaactggga
    20101 tacatggtgt tcggtcttgg cgtaggtgcg tactcggcgg gccttttcca cctgatgacc
    20161 cacgcgttct tcaaagctct gctgttcctt gccgcaggaa gcgtgatcca tgcgatgcat
    20221 cgcgcgtacc accatgcggg gcttgaatgg aacccgcgat cccgtgagaa tgacgcccag
    20281 gacatgcgca acatgggtgg tttgcgaaag gccatgcctg tcacgtttgc caccactgcc
    20341 gttgccgtgc tggcaatttc tggagtgccg ttcaccagtg gttttctgag taaagacatg
    20401 attctcggag gcgcgtttgc tttcggtttc gttgagaatc cacagcactt cctcattccg
    20461 gtgttcgggt tcgccgccgc cgtactgact gcattctaca tggcgcgtct gctgatcatg
    20521 acgttcctcg gcacaaaccg gatggcggaa cgtgggcaag aggcgctgcg ggaaagcccg
    20581 tggaacatga cggttcccct tgtcctcctc gctgtgttgt gtggctggtt ctggtacaat
    20641 ccggacccga caacgatttt cgcgaagggg tgggtgtcat ccttcctgac gccacccgct
    20701 tcggctgtcc cgcaaacgct tgtagctgct cccgcagacg cccatcatgc cgcgcatgtg
    20761 gcagcgatgc tcgtgtcact gtcctgtgcc ggagccggtt ttttcgccgc atggatgctc
    20821 ggtcggcggg gcgcgatttc gttggagcac tggagactgt cgggatcgcg cgtgtactac
    20881 cttctggcca acaagtatta cgttgatgag ctttacaaca ggaccgttgt cgccggaatg
    20941 gttcaggttt cgcgcattct agggtggttt gacaaggttc tggtagacgg agttgtcaac
    21001 gccacaggtc gcttccagac agggttgtcc gaactctccg gtttggtcga ccggtacggt
    21061 gtcgacggcc tcgtgaacgc cttggccggt tttgtgcgcg atacgggcaa tgaagtgagg
    21121 cagatccaga caggacgcat tcagaactac gtcgtgtacg caacggtggg gctcattgtg
    21181 ttgatcgtgg ttaccggcat gtgagggcac tccggatatg tccaacatcc tttcgtggtt
    21241 gacatttctg ccgctggttg gaatggcggc gatcctcgtc attcctacga gattgcgcaa
    21301 aagcacagga ttgatcaggt acgttgccct cggcacaacc ctggggtgtt ttgctctcgc
    21361 ggcgtggctc ttcttcgcat ttgaccgcag ttccagcgcg atgcaattcg tcgaaaatgc
    21421 cgactggatt ccttacttct ccatccgtta cacccttggc gtcgacggcc tgtcgatcct
    21481 catggtgttg ctgacacaga tcatcggcct cgttggcgtc atcgcgtcat ggaacatcaa
    21541 caaagcagtg aagggctatt tcgcgctgta ccttttgctc cagaccggca tgaccggggt
    21601 attctgcgca ctcgacttcg ttctgttcta tgtgttctgg gaagtcatgc tgttgccgat
    21661 gtatttcctg atcggcatat ggggaggccc ccggaaagaa tacgcggcaa tcaagttctt
    21721 cctgtacacg ttgctgggaa gcgtattgat gttactcgcg atccttgcgt tgtatctcaa
    21781 catgcaggcg cccagcttca gcatcaccgc gatggcagac cccgccaatt tccgggccga
    21841 cggttggctt gtacgcaaca tgccaacctg gttctttggc ctcaccccga aggaacttgt
    21901 ctggcttgcc ttcttcgtgg gcttcgcgat caaaatacct gcgttcccgt tccacacgtg
    21961 gcttccagat gctcacgtgg aggcgccaac agccataagc gtcatcctcg ccggcatcct
    22021 tctgaaaatg ggaacgtacg gcattctgcg ggtcaatttc cccattcttc ctgcggaaac
    22081 cgtgcatttt gcgaccttta tcgcggtaat cgcagtaata aacatcgtct acggcgcgct
    22141 gtgcgcgatg gcacagaagg acctgaaaag ccttgtggcg tattcctcga tcagccatat
    22201 gggttacgta atgctgggca tggtttcctg gcgtaacgaa gcgggattga acggcgccgt
    22261 gctgcagatg ttcaaccacg gcacgatcac cgccatgttg ttcctccttg tcggcgtggt
    22321 gtacgaccgg gcacatcatc gccgtatcga tggtttcggc gggctctggg caaaggcccc
    22381 cgtgttttcc agcttgttcg ttgttgcttc catggctgcc ctggggctgc ccgggctttc
    22441 cggtttcatc agcgagctcc ttgtgttcat gggtgccttc gccagccacc gcgtgttcgc
    22501 tgtaatagca tcatccggca tcgttctcgg cgccgcgtac cttctctgga cgatccagag
    22561 ggtcttcatg gggccactaa atcaggcata cgcagagaag gacgcaatgc ccgaaatctc
    22621 gccaagggag atcgcttccg tcgttccgtt gatggcaatt gtgatcgtgc tgggcgtgta
    22681 ccccgcgccg gcgctgaatc tcatcggccc gacgctgggg tcgctcctcc agacaatgca
    22741 aagcgccgct ggcgcggcga tcctccccta ggccggaggg tcaggaagcg atgggttcga
    22801 tccaggatca catcatatca agcgtgcacg ctttcgcgcc agagggtgtc ctcgcggcaa
    22861 cacttctcct catcatcgtg gcggatctgt tgccggccac ctcgcgcccg gctgtttccc
    22921 tcgggctggc cctcggaggt ctcgcggtat ctgcggctac cgtggtgcgc atacctgccg
    22981 gggagacact catcttcgcc ggcatgctgg caacggatgg cttcgccgca acgttcaaac
    23041 tggtcgcagc ggccgttact gccgtagtgc tggttgtttc gtatcgcgca accgacgtca
    23101 acagggagca cttcgcggaa tactgcgcga tggcgatcgc gttgtgtctg ggcacgtacg
    23161 ttatggtggc agccgcaaac ctgttgagca tgttcctagc atttgagctg gtcagcatgt
    23221 cgtcctacat actgaccgcg cacatgaaag aagacaggaa agccagcgag gccgccctca
    23281 aatacgtcgt cttcagcgca tcctccagcg cagtcatgct gtacggcatg tcattgatat
    23341 acggcctcac cggcacactc gtgctgaagg caatcatgcc tgcccttgcg ggcaacactg
    23401 cctcccttgc acagtctgtg ggcgtacttt tcgttctggc aggcctcact tacaaaatcg
    23461 ccgcggtccc gtatcatttc tggtgcccgg acgtgtacga gggagcaccc gcgccgatcg
    23521 cggccttcct ctccgtgggc ccaaaagcag cggggatggc gatgcttgcc cgtttcttga
    23581 acgaggctct cttccttccg ccgggttctc ccattcggtc cggaatcgat tttggcaact
    23641 ggcagaccgc aatcgtgctc atcagtgcgg taaccatgac cctcggcaac ctttcggcgt
    23701 tgcggcagac gaacgtcaaa cgccttctcg cgtacagctc gatcgcccac gcagggtaca
    23761 tgctgatggc gctggcagtc atcaggccgt tcggcgttac cgcgatgacg ttttacgtcg
    23821 ttgcgtacgc actcatgaac ctgggtgcct tccttgtcgt tatcgcgctc ggacggagta
    23881 tgcgcagcga agaacttgac tcgtacgccg gtctgggagg tcgcgccccc gttgttgcgg
    23941 tattgatggc gatctttctg ttttccctcg ccgggatacc cccgaccatg ggatttgtcg
    24001 gaaagctgta tcttttctgg gccgttctgg aagggaaagg gctcgcctgg ctggctgttg
    24061 tcgcggcatt gaacacggta atcgcgctgt actattacgc gcggattctc cgtcaaatgt
    24121 tcttcctccc cgcgcgagag ggagctggag agattcgcgt agagccgatc cattacgtcg
    24181 tcttgttcct gctggcgatc ccgacgttgg cgctcggtgt gtactggggc ccgctctggg
    24241 gagcgatttc cgccgccggg acaccgtgaa gcagcgggaa ggctccggaa tgggccatac
    24301 tccactggca atctcgcttc tcgtctgcgt cctctccctc caatctgctg cggcagagca
    24361 tgttacccgc acacgatggt tcttttcctt cgggccgtcc gcaatcttcc tctcgaacgc
    24421 tgcgcacgat gatctcgcgg gggtttcggc gccggcaggc acaggtcggg gtggctccgc
    24481 ccagctcggg ttcgaggtga cttctcactt cgctatgcgc cttgcagttc acagcgctcg
    24541 gcacagcgtt gcgggacccg ggacaggagg caccgcttct gcggcggtgg atttcgtagg
    24601 cattcagaag tacggagcta ttgcgccatt cctcttcggg agagtcggca aacaggcgtt
    24661 cgttttgtcg aacgcccgga caactacctc agcgcccccc cgtgatcatt cctggaacgg
    24721 ccatcttctg ggcggaggcg caggggtgcg gctgtacttg acgcggcgcc tcagcgtcgc
    24781 cccggaggcg tctctgtgct gggtgcggta caataaggtc atgagcaccg acggaacggg
    24841 gacgcaattg ccgcttgatc ccattagcgg cacgacggga tcagtctccc tcctgttgag
    24901 tatccactgg taatcgaaac ttcatccctg cggttcggga acacgcttca ggcgctggta
    24961 gctccttgag gcgactcgaa ccatcgtccc ttcagataca gggatacgtt gacgagacca
    25021 atcagcaccg gaacctcgac gaggggaccg atcaccgcgg cgaatgcggc gccgctgttg
    25081 atgccgaaca cggcaactgc aacggcaatg gcgagttcga agttgttgct cgctgccgtg
    25141 aaactgagcg ttgcggtctt cgcgtaatct gcccccgctt tctttccgat ccagaagctg
    25201 atgagaaaca tgatcacgaa atagatcaac agcggtattg ctatccgcac cacgtcgaga
    25261 gggatctgga cgatcaggtt gcctttgagg ctgaacatca ccacgatggt gaacagaagg
    25321 gcgatcaacg taatgggact gattctcgga atgaactgcc cgtggtacca ctccttccct
    25381 ctggatttca ccagaacaag ccgcgtcagc gcaccggcga tgaacggaat ccccaggtag
    25441 atgaacacgc tctcggcaat ctgcccaatg ccgacgtcga caacggctcc ccgaaggccg
    25501 aacagcggcg gcagaaccgt gatgaagaac cacgcgaaga cgctgtagaa gagcacctga
    25561 aagatgctgt tgaacgccac gaggccggcg cagtactcag tgtcacccct cgcaaggtca
    25621 ttccagacaa tcaccatcgc gatgcaacgc gccagaccta tcataatcag gccgaccatg
    25681 tactccggat aaccagacag aaacgtgatc gcgagcacaa acatgagcac gggaccaatg
    25741 atccagttct gcacgagcga taacccgagg atcttgccgt tgcggaacac atcgcccagc
    25801 tcctcgtatt tcactttcgc cagcggggga tacatcatca ggatgagccc gatggcgatc
    25861 gggatgttgg tcgtgccgac ctgaaaacgg tgaatgaatc cctcggtacc gggcacaaag
    25921 tagccaaccg ccacaccggc ggccatcccg aggaaaatcc agagagtaag aaatctgtcg
    25981 aggaacgaca ggcgcttggt tgcgacggcc gacatagtgt tctcctttgg gactcacgga
    26041 aaaccacaca gtacagtaat cgagtttcta tttccagagg gacagagtag ccgaaatcag
    26101 ggccgctcac tcgcagttgc cgccacagga gcattgcagc gtgcgctccc ctcgacttcc
    26161 gtccgcttgt ttccctctgg acgtgattgt tccgagccct ccggagatta ctcgcctgac
    26221 gggctcgccg gtttctggat gcgtgacaag tggttcgtcg ctcatcctct gctggatctc
    26281 gaaccttttc ggtttctccc ccggtttgcc ggggatcgtt tcatacacgt aggtagccat
    26341 tgcgtcgccc tctgtggcgt cttcggtgac ttcttcgatc tgtacgttgc agcacgagga
    26401 tttcttcggt ccaaacaaca cggacaacac ccctttgccc ttcctgtcag ccatcaatcc
    26461 actctccttc agatcacgtt gaacacaaca cccgccagca ctgccgtaac caacactact
    26521 gaaacgaacg tcgccaccag gcgaggcttg aagatgctcg ccagcatact catctcaggg
    26581 atgctcatgc cggccccgcc aataatcaaa gcgattactg ccccgaggct catgcctttc
    26641 tgggtcaagg ccacggcgat aggaatcacc gtttccgcac ggacatacag cggaacaccg
    26701 atcactgccg caacagggat agcgaatgga ttctgcgggc ccgcaatacc gacgacgaaa
    26761 tcacccggaa gataaccgta aatcgccgcg ccgatcgcaa cgccgattac gaggtacacc
    26821 agcaccccgc ggaaatcccc ccacgctccc gcaaaggcct cgcggagttt tccccggaac
    26881 gtttccggtt tctcctgatc gtgcaccaca ccgctcaccc ggacattcct gacgtccgat
    26941 gccaatccga agccttgcaa ggccgctccg caagccatgg atgcaacaaa cgttacggcc
    27001 ccgtatacca cacaggcttt ccatcccagc agaaacaaga acatggtcag gatgatcgga
    27061 ttcagcaacg gcgacgcaag tacgaatgac atcgtcgcac caaagggcac gcctgccctc
    27121 aaaagcccga cagtcatcgg gatcgtggaa cacgagcaga aaggcgtaat cgctccaagt
    27181 gccgcaccga ggaaattacc aagcacacct ttcctcgaaa gccacgtgcg cagtttctcg
    27241 tcagacacgt actggagcat caggcccact aacgtgctga tgccgaggaa cagcgctgtg
    27301 agttccgcga gaatgaagac gaagaacttc agggatacag caaacgggtt catcaatcca
    27361 ccttttcatc gttcacccag ttgcaatccc ggaccgtcgc tatcggacaa ccggttgctt
    27421 caccggaacc gtgacaacag gcaatagcta ttcaggtttt ctcccgcgaa tgatcgctga
    27481 tgcgacagag ttctcgattc cgctcccggg tacccagcgg ccgatcagtt cgcggctttc
    27541 ttcctcaatc tcaacacgga tgtcctggaa tccagcgttc tccaaccatg cacgaacctc
    27601 atccaccgag gccgccccgc cgatacagcc gcacacgagg tcatcatccg tccgcatgct
    27661 ctc
//
LOCUS       DAOSOO010000015        27636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1743_length_27636_cov_5.462529, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000015 DAOSOO010000000
VERSION     DAOSOO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 27636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27636
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_27636_cov_5.462529"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLT86_02195"
     CDS             <1..518
                     /locus_tag="PLT86_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT35456.1"
                     /translation="SVKEGPRARGCAGMLSHAPWVYDRMSVRDNVRFFAKLRGKPLSD
                     GEILDALADMDLDNVADADAGTLSRGMKQRLGIVRAFLGTPALLLMDEPFTGLDRKSA
                     GLLCDRMIRFREAGGTLLTVTHAVSEGWRIATRALMLHRGRLVQDVPVTPDGLESFAA
                     QYDDRMRGEDD"
     gene            518..1186
                     /locus_tag="PLT86_02200"
     CDS             518..1186
                     /locus_tag="PLT86_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOT35457.1"
                     /translation="MRVFWAILWKDIRYELRRRELVAVMTVFALLVVVIFAFAIGIRS
                     ESPASMAASVLWVATSFAGTLGLNRSLALERDEQCLKALLMSPADRTWVFLAKATGNL
                     VFLLAAQVILTPLVIVLFGVEDTGHWSLLGVTLLLGSAGFVGVGTLFATMATSTRMRD
                     WLLPILLFPVEVPVLIAAVKATAHVFSTAPPGESTIWIAILAAFDLIFVSMSILSFGF
                     VVEE"
     gene            1187..2572
                     /locus_tag="PLT86_02205"
     CDS             1187..2572
                     /locus_tag="PLT86_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT35458.1"
                     /translation="MTVEVITRMNERNGTSGVTLVVGAGLAGLTAAYRWRQRGEDVVL
                     AEKAPYVGGMARSHYRNGFTFDFGPHFIIEKTPLLDELFAPDELVAFGPEIHMVLGGR
                     WIRYPFTPSSMLKVPLGVQMDFLRTRALGPRPGVDYGPPDSFRRNMSRQFGRGLYDFF
                     FRPYLTKKFGGWDISDVVHSDWWAMATHTRGVVTRFAPPAPQTGHAVGALRKLARLTK
                     ELPYVFRKLALWYPKAGFGEIPKRIAEQFTHNGGVIRLNCQISQIRRTENRITGVVVD
                     GEEIPVRRLIWTASPNLLARMLGTSSLDMPTLHSIIYFISTDRRYRRRGTEVRPVDPE
                     FAFYRAYFPETIWEGLTPGGRSAVVAEVGTTNRDDLAKAGEKYGAIIDTCVALGLFPR
                     NAVTDIFHDVQPDCYPVYPLDYETHLQKYYSELTPIQNLLLAGRNARFQYYNSHQVIR
                     DGERTVEDVLF"
     gene            2823..4202
                     /locus_tag="PLT86_02210"
     CDS             2823..4202
                     /locus_tag="PLT86_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT35459.1"
                     /translation="MSAASMEEPGSSTLEAASRTSPDDIIDPRHEKRNYWLTVINGIM
                     TGGPSTLFDPNTVVAAFIIHLSGSQALVGLVSGLVMLGHVWPQLFVANWIEPKPKKLF
                     VYHISTVLRLASLTTLGVLIWVFRDDLPGWFVYAMIGLLFAFWSAGGFSAVAWYDILS
                     KTVYSYKRPVLFAWRQTGAGIIALLAGGVITWALSPRSGLRYPLNYLFLLSLMTVLIA
                     IGILCFNLVVEPAETHAIKKRRPFREYLRLGPQIMREDHNYRRLFWGQITFSLAVMNT
                     PFLVPFLIKDIHVDDSIIGMLMVVAAIADLLINVYWGQLGSRKGNRAVLVLGSRLSAF
                     SPALALGAVLLPRFALFGVDIRIILVALSLVMSRIAGSGLGVGRMNYLLDVAPLGMRP
                     SYMGFMNTFSAVSMIIPMTAGSAIQLVGYWPVFALAVIFGIVCVRVTTNLDDVKFTSH
                     PNGYRPEES"
     gene            4282..4905
                     /locus_tag="PLT86_02215"
     CDS             4282..4905
                     /locus_tag="PLT86_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOT35460.1"
                     /translation="MAKIKVLQFAGGEIHDWKGVGDEVEATLKEDSEFEVTRVNEDLN
                     VFTTDLSAYDVVVFHYTVGSITDAQRDGLFNFVKSGKGFVGIHSAADSFRDSPEFRDF
                     IGGYFVTHPRYRSYQVSVLDDKHPITEGLDEFVVTDEQYITDYDPRVNVLATGLYKGK
                     AYPVIWTKSFGKGKICYNALGHDANACKNMYFKLTLVRGVKWAATTA"
     gene            5274..5885
                     /locus_tag="PLT86_02220"
     CDS             5274..5885
                     /locus_tag="PLT86_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT35461.1"
                     /translation="MRCKVTPETFAEAQSGSHRAFARLHEEVEPCVRWFASRLIGLPW
                     VDDIVQKTMTALYINIGKIACVESLLPYVFRVVRNFCYEDLRREGRFNLPPRQRRGQN
                     DEERDPRHVRDQNRLADEAAGVLELAHEVARALNELPELQREALILFAEGDLTYAQIA
                     EAMHSDIGTIKSRIFNARKNVIKRINPRVLDELGIGKEITNGV"
     gene            5875..6423
                     /locus_tag="PLT86_02225"
     CDS             5875..6423
                     /locus_tag="PLT86_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35462.1"
                     /translation="MASENDKLRALIRMLPPARALRQELKAVIAAETFDGVGRLAVRN
                     FTGLLNSVMSLTDDGTYLDTLNVEPSPNGGTADKEQVMAVLFSLSQLIAYLEGQIGLP
                     ASGGEEVSTDNRSYINNDNSVDMRGAVFSPASGNPLIDPDSVGKLIGGERGQKVADML
                     RSVFEARAAKQDEGTDDGEEEA"
     gene            complement(6630..8042)
                     /locus_tag="PLT86_02230"
     CDS             complement(6630..8042)
                     /locus_tag="PLT86_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT35463.1"
                     /translation="MYFYWFIIMIFTLFQASAWVNMPSLPRMEDASLATTVREDLPDI
                     QKRDTLRVITQHSASSYFLFRGDERGLEYELARQFADMLDVHLQMVIPPTPEDMIPWL
                     MEGKGDIIAPGLLTAVPNHAVALRCLPYDSSYFVVVTRGSAAVASLDELAGRTVHAIP
                     SGPGYYALVDLNTQLGGAVTIAPAPSGWDYEMLLAALSKGEAEAVIAPWPLAKIERSY
                     HQNITIGPRIGQNEPVGWYVRPSANQLRDAVDVFMSRVQSSGITATLRAKYFDKGERA
                     ARYRAASARFLREGQISPYDHTIKLAAEEAGLDWLLVAAVAYEESGFAHDMESPMGAV
                     GLMQIMPETAASLGVADVLDPVQNIHAGTRYLRDQIAQFGSLEKRDAIAFGLAAYNVG
                     LGHVEDARKLAAEIGRDPNRWRGNVEAAMELLAHQRFYRRASSGYFRGSETARYVNAV
                     LARWETYERMMRRVGSQPAA"
     gene            8636..9352
                     /locus_tag="PLT86_02235"
     CDS             8636..9352
                     /locus_tag="PLT86_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT35464.1"
                     /translation="MESAQDLHARIKLAVDHVREEIADAAVKSGRSPDEIALLAVTKT
                     RSPAEVNAAIGAGVTLLGENRVQEAEAKIPYVAGGAEWHLIGHLQTNKARLAARLFRM
                     VQSVDSVRLAEKLNDAVESGRSLDVLVEVNVSNEASKFGVAPGSVGELVEKLVELPAL
                     RVRGFMTIGAFVDDERLVRRGFRLLREIFDDTRRQFPSGQVDILSMGMTGDFRWAIAE
                     GSTMVRIGTALFGQRTQRGA"
     gene            9352..9636
                     /locus_tag="PLT86_02240"
     CDS             9352..9636
                     /locus_tag="PLT86_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOT35465.1"
                     /translation="MFQWQFFLNGVLRLLILLLKIYSIIIIVRVVASWLGADPYNAIM
                     NFLSRITDPFFEAIRRRLPSAMWNTGLDFSPLIAVLLIQVVTLLLESIRF"
     gene            9674..10495
                     /locus_tag="PLT86_02245"
     CDS             9674..10495
                     /locus_tag="PLT86_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT35466.1"
                     /translation="MNITPLDIRRNEFTRALRGYNREEVDSFLVMVADEVESLISEYR
                     DLSRKLEEQEKQLENFRMIERTLMETLITAQRTSDNMRETTQRECDAIKREAELKAQE
                     TIEKARLEAERLAMEARQRAHNVLEDARTKAHNAIEAALKQSDEIYQAAREEAMEIQT
                     ATKVMIERRDSFILQMRAFLGGQLEALDRFGHEELAAPPAVQEHEVPIITEEEAAAIA
                     ALDKELSAFAAATAPPEESVPVSEPPVREEAPAEEPPEDGAEVLVGRQLPRSREA"
     gene            10501..10788
                     /locus_tag="PLT86_02250"
     CDS             10501..10788
                     /locus_tag="PLT86_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOT35467.1"
                     /translation="MQRDAEPLHRVTVHVQPRASKNAVGEMDAAGRLRVAVTAAPADG
                     QANEAVIALLADHFRVPRGAITLERGKTARTKIFALRGAQISAPAHDGNAT"
     gene            10785..11618
                     /locus_tag="PLT86_02255"
     CDS             10785..11618
                     /locus_tag="PLT86_02255"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT35468.1"
                     /translation="MNTPEPKLSEQIREALAVVQARSSLKPSVGIILGTGLGQLADEI
                     AVETVIPYGEIPHMPLSTVETHAGRLLIGHLGGKPVVAMQGRFHYYEGYTMKQVTFPV
                     RLMRAMGADTLLVSNACGGMNPAWEPGDIMIMTDHINLLGDNPLIGPNDDSLGPRFPD
                     MSEPYSRELIELCRAIAREHGIPVRAGVYVAVPGPNLETRAEYTFLRKIGADVVGMST
                     APEVIVAIHGGMRVLGLSVITDRCLPDSLEPATIEQIIAMANKAQPGLTTLMRGVVER
                     L"
     gene            11770..14946
                     /gene="ileS"
                     /locus_tag="PLT86_02260"
     CDS             11770..14946
                     /gene="ileS"
                     /locus_tag="PLT86_02260"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT35469.1"
                     /translation="MYRDVPPTFSLPELERRILAFWKEHDTFNKLRRKNENGPRWSFI
                     DGPITANNLMGVHHAWGRTYKDAYQRYHSMLGQHLRYQNGFDCQGLWVEVEVEKELGF
                     KSKRDIEEYGIDRFVEACKARVIKFSKRQAEQSIRLGYWMDWDNSYYTMSDENNYTIW
                     HFLKKCHDRGFIYAGHDVMPWCPRCGTAISDQEIVTEGYQELTHTSVTAKFPLVGRPG
                     ESLLVWTTTPWTLTANVAAAVHPELTYLAVKQGDETFYLAKACAERTLKNEYAVLREL
                     PGSELLGWAYSGPFDELPPVAGVEHRVIAWDEVTEEEGTGIVHIAPGCGKEDFALGKQ
                     FSLAVIAPIDENGLFAEDFGPFAGKHASHVPEDVFASLKNKGRLYRTQKYSHRYPVCW
                     RCKTELLFRLVDEWFIDMSTLRYEIMEVTKRITWMPSYGLDHELDWLKNMGDWCISKK
                     RFWGLALPIYPCQKCGNVDVIGSETELEQRAVEGWEQFAGHSPHRPWVDAVKIGCSKC
                     GSPVSRIKDVGNPWLDAGIVPYSTLNYRHDREYWNNWFPAEFITESLQGQFRNWFYSL
                     LAMSTVMENREPFKRVLGHAQVRDLKGEEMHKSKGNAIWFDDAAEKMGAETMRWLYCR
                     QNPYTNLNFGYEAAEEPLRHLTTLWNTYKFFVTYAKIDGFVPGSSPVPYDRLDRMDRW
                     ILARLNQLIADARRAFDGFSVMNFVHAAERFIDDLSNWYVRRCRDRFRQADQAGDPAA
                     YETLYTVLSTTVRLLAPVIPMFAEELYQNLVAWREGEPESVHLTPYPEETPEWEDAAL
                     VADMALVRNVVEIALAARNAAKIKVRQPLAVMTVVTDPSAIKTLEMNRQVIAEELNVK
                     RIVFTDSAEGLTTRVAKPVFRALGPRFGKDANAVGNILRALDQDTLARIAAGETVTIP
                     VNGKHEQISSELVEVVTASPQGMSVISDKQVTVALDTNLSDELVDEGFAREIVNKIQN
                     MRKEADFNVSDRITVGYSVSPRLARAIDSCRTYIMKETLARELVPLEGEWEHRSEWSV
                     NGEPAELGVRRTTQSGLANGGGMQDGRT"
     gene            14933..15322
                     /locus_tag="PLT86_02265"
     CDS             14933..15322
                     /locus_tag="PLT86_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT35470.1"
                     /translation="MAALDPQELAYFEKLLKERRAKALSAAEEGESVRPKEAPKESSG
                     DLSAYSVHMPDQGTDAMERELEFLLAARDEKYIRHLDEALQRIKDGTFGICRTCNKPI
                     SRARLEAVPNATECIACKSQRDKLPRR"
     gene            15357..15872
                     /gene="lspA"
                     /locus_tag="PLT86_02270"
     CDS             15357..15872
                     /gene="lspA"
                     /locus_tag="PLT86_02270"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT35471.1"
                     /translation="MFIGPALASLGLDRITKIAAERHLTFSVPEKVLGEYVRVVLWFN
                     EGAAFGMTFGGKWAHVILSLIAMVVVTYLVWRTPREHSLMLSGFALILGGAAGNLWDR
                     LLAGKVTDFIDVGIGSLRWPTFNVADASVVVGVFLILLTSWRTSRAEQTAKETMARPA
                     QSAATEGTPAP"
     gene            15869..16354
                     /gene="lspA"
                     /locus_tag="PLT86_02275"
     CDS             15869..16354
                     /gene="lspA"
                     /locus_tag="PLT86_02275"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT35472.1"
                     /translation="MKTPLRAADYRLLLSLGLGVLFTDWLTKALAANLLSAGRPVSLF
                     GDYARLSLSHNPGAAFGMRFGGAAVHLLVTLAVIAFIVAIALHTAPSRRLEQAGYGLI
                     LGGAIGNLGDRLLLGHVIDFLDLGIGNVRWWTFNVADAALTAGVGLLLIASFRSRRTV
                     Q"
     gene            16951..>18387
                     /locus_tag="PLT86_02280"
     CDS             16951..>18387
                     /locus_tag="PLT86_02280"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF010322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOT35473.1"
                     /translation="MPRPNIILIMCDQMRGDAFGADGNSSIETPNLDFLASLGTRFCH
                     AYSAVPSCLPARAILWTGQNQWHTGVLGMGRGQGQIPHDFPHTLAGELANAGYRTHLV
                     GKGHFHPQRAPMGFHDMELDESGRMPTSEHRQWFRTHAPEGVTPDDHGVDWNSWHSRP
                     WHTDEYLHPTAWTMSRALNFLDTFDYSQPFFLNISFARPHSPYVPPRFYWDFYHSRGT
                     PPPSVGEWAIMHDDPRTALDPNAWRGKISPDRIHRARSGYYGEISFIDTQIGRLIKRL
                     NRGNDHASFANTWIVFTSDHGDMQGDHHLWRKTYAYEGSARIPFVIVPPRALGRPSRQ
                     VADEVVELRDIMPTLLEGAGVALPGTVDGQSLVPLLDAPAAEWRPYIHGEHCTCYSEE
                     QEMQYVTDGRRKFIWLPRINVEQFFDLECDPGECHDLIHDPLRQTEIATWRGYLVNEL
                     AKRDCGWVVDGKLHCPPDPLVSPWKDVRW"
     assembly_gap    18388..18487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18488..19768
                     /locus_tag="PLT86_02285"
     CDS             <18488..19768
                     /locus_tag="PLT86_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT35474.1"
                     /translation="SILWSGQRQWHTGVLGQGRGQGPMPNDFAHTIAGEFASAGYRTH
                     LVGKGHFNPHRASMGFQTMELDESGRMPDSEHRQWFRTHAPEGVTPDDHGVAFNSWHA
                     RPWHTEEHLHPTAWTMSRSLAFLESHDSTQPFFLNISFARPHSPYVPPRYYFDLYHNR
                     ETPPPHVGEWSARNDDPVTAVDPNAWRGKQTPEAIHRARAAYYGEVSFIDTQIGRLMN
                     WCSRYRRAAWENSWIVFIADHGDMQGDHHLWRKNHAYEGSARIPFIVVPPKEFRGAKR
                     AVAYEVVELRDIMPTLFEAAGIPIPPTAEGRSLLPLLDAPASDWRPYIHGEHSQNYSP
                     EQEMQYVTDGRRKFIWLPRINVEQFFDLESDPGERVNLIDDPSRQSEIATWRGYLVKE
                     LAERDCGWVVDGRLHCPPDPLVSPWKDKRWMGTR"
     gene            complement(19930..20751)
                     /locus_tag="PLT86_02290"
     CDS             complement(19930..20751)
                     /locus_tag="PLT86_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT35475.1"
                     /translation="MNHATPLMEEFRRLGKSVSCPIIDMHGHFGPFNGIFFPNVTAEQ
                     MVGSMDRAGVRLIVCSSHEALLVNSDTGNARMADVVRRFPDRFRAYWSVNPNEPDVLL
                     RQLDGLAERKEFVGIKFLSDYHRYPITGDAYVPALKYAQEHRMPVLMHTWGGSKFDGP
                     ELVGEVADRYPDLVILMGHSGYGEWDVSISVARKHPNVYLELTAAYAVNGVVEKLVNK
                     AGSEKVLFGTDLPWFDPHYAAGCILYAHISDNDRHNIFHRNAERILGQCGNPVRF"
     gene            complement(20748..21509)
                     /locus_tag="PLT86_02295"
     CDS             complement(20748..21509)
                     /locus_tag="PLT86_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT35476.1"
                     /translation="MTIIDANASFGAVYTPTLAPCRTPEELIAALDRAGVDRALVAAD
                     AIRGGCPSEMNVRAALELAPHARLMPVWAILPLETGEFGTRTEFLDSMKRYGVRALAA
                     YPSKHRYLLDRVGCGELLDAMVECRIPLLLPLEESSGGVGGWALAEKLLSETPGLRLI
                     VLGTGPWGDDRLFRPLLRSFPTLTVEVSRYELDGGIAALCGKYGAERLVYGSGYPHWA
                     MGGALLTALHADIPEADKGLILGGNIARMLDEVTI"
     gene            complement(21577..22761)
                     /locus_tag="PLT86_02300"
     CDS             complement(21577..22761)
                     /locus_tag="PLT86_02300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT35477.1"
                     /translation="MYRMGQEEVDEVAKVILAKQLFRVGDPKAGHLQEVDRFEKEWAE
                     KIGTDYALMVSGGGTAALICGLAGMGIGPGDEVIVPAYTWLATASAVLSVGGIPVLAE
                     IDETMALDPDDFERKIGPHTKAVIPVHMLGRPADMERIVAIARRHSVKVLEDSCQNNG
                     GMYKGRRTGTWGDAGAFSLNYYKIISCGEGGILVTNDEKIAERAVIFHDSGTAFRPKA
                     GELTEPIFIAQQYRASEVMGAIARIQLQRMDGIVADLQRVRRTIEAALAGEPGLRFAP
                     SNDPDGDSGVVVAFQFDTEAKARAFQQSPGVGGYVGIDHGKHVYTEWTPIREKRFMHH
                     PAMNPFNFPQNKGLRADYSDTACPRSLDLLRRTVFCSLDPDWTEQKVAEKIAAFKMAA
                     KG"
     gene            22950..>23761
                     /locus_tag="PLT86_02305"
     CDS             22950..>23761
                     /locus_tag="PLT86_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35478.1"
                     /translation="MTPVLFSVSYAGLWGQDTLDVESFIERAAKLFYPAVELMGKRPH
                     LSVLDASPERIRRIRERATATNVEIVTIAAYTDFTAGPAPEVPLGEMQVLYVRELARI
                     ARLLDAHVIRVFTGYSTDDHSYVTDWKKCVAGIREAATVAADQGIVLGVQNHHDTAIS
                     TDAYVEFLDEVNHPNCKAMYDPWVPALLGEDLYACAKRLAPRMVQTTLADYVRIPRWA
                     YEPGLVNYRKLPDMVRAVPLGEGFIDLNAFFRGLKEGGFDGYVAYEMCSPLRG"
     assembly_gap    23762..23861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24087..26501
                     /locus_tag="PLT86_02310"
     CDS             24087..26501
                     /locus_tag="PLT86_02310"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT35479.1"
                     /translation="MKHETTQLEVTPTGTVGISCPSWPGFAVRGLSPALRVDGADLKP
                     RSAHVATNTDGSTVVEYRFREGLSLTCHVEPWRNCGYRFHTVLHNNGGTATVLNNVVL
                     LGTDGEGFRVAFGAADDPVRVMEQGNYWGKVRPLSAPKAVPAENAQGEPNEAPGTTHG
                     SSDFVWVAYNQASRMAFLAGFLSSERWSGRISVETAPGGEVLHWGIGFDGADVLLKPH
                     SETPLEDLLFLVGADPWELLASYAGEVQTIHNPQFPAVPPVSWCSWYAYRLGVSEERI
                     LENARIAAQRLKPLGLGIIELDLGWEAGNLPSTFEENERFPHGLKWLSEELAKLGFDM
                     GVWKAPFTISEFDPMAKEYPEYLIQGEDGKPAPYWDWFWEPHGKVFILDLTHPGAQAW
                     LREKMTSLAERGVKYLKSDFIGCVTGEAAKRRHNPDIAAGGGLEAARIGARIIRECLP
                     DALQLNCGGPEMPGTGHWPLLYTVQDTGNTGFIGHSFQRDNYQAAAVHLFKNYRWGIL
                     QPSCLCVGAPGTLDDARLRATIAFLTGGQVDISDTLTTLPEDRWAVLTATLPPLGITA
                     KAVDLFDPVYAVPFDYEAICRGEASGDATPKEHPPGSVWKLHVKTDWDEWDLVGVFSY
                     SPGASAETPAMDRFSIPFSMLEIPPESTRWGYEFWSGQFIGTVPGKRTNDRGYVHPGD
                     YQDLTVGDAPGVLEIAFFGPCVKLLALRAQRPHPWVVGTSFHQSCGAELHNVAWDATG
                     GVLSGEVHRPVGETGFVALSTAGMATVSCRVDGIAVPHRTGANGSLILPVTMNADRAE
                     WQGRFG"
     gene            26513..>27636
                     /locus_tag="PLT86_02315"
     CDS             26513..>27636
                     /locus_tag="PLT86_02315"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOT35480.1"
                     /translation="MAVVTLERAYYSAPFGDFFGATDDELLGHLCRRSELDVEQYQRN
                     AWIAEFNLLRRHLFGFSGHILLEYAIPRMGRRADAVLLLPRFVVVLEFKVGQATYSPD
                     GLDQVFDYALDLKNFHEGSHEKWVVPILVATEAPEQSSTFRRFEDGLFAPARANAANL
                     AAVLREAAITSHEAPPVDVAAWLNAGYRPTPTIVEAAQALYRGHNVVEIARSEAGAEN
                     LTRTAETIAGIANRSREQGIKSICFVTGVPGAGKTLAGLNIANSWHDPESGEHAVFLS
                     GNGPLVEVLREALIRDDVQRASAAGGKRTRSASQSRVKAFVQNVHHFRDEALATFDPP
                     VERVVVFDEAQRAWDAAQTARFMKSRKGVLSRMSAAKVLVG"
BASE COUNT         5453 a         8394 c         8289 g         5300 t
ORIGIN      
        1 attcggtgaa ggagggtccg cgcgctcgcg gatgtgcagg catgctctcc catgcgccgt
       61 gggtgtatga ccggatgtcc gtgcgggata acgtgcgctt tttcgcgaaa ctgaggggaa
      121 agcctctcag cgatggagaa attctggacg cgctggcgga tatggacttg gataacgttg
      181 ccgacgccga tgcgggaacc ctctcccgcg gcatgaaaca acggctcgga atcgttcgcg
      241 cctttctcgg cacccctgcc cttctcctta tggatgaacc gttcaccggc ctcgaccgga
      301 agtccgccgg cctcctctgc gatcgcatga tccgcttccg cgaggcaggg ggaacactcc
      361 tcactgtcac gcacgcggtc agtgaaggct ggcggatcgc cacccgcgct ctgatgctcc
      421 accggggaag gctggtgcag gatgtcccgg tcacgccgga tggattggag tcgtttgcgg
      481 ctcagtacga cgaccgcatg cggggagaag acgactgatg cgggtgttct gggcgattct
      541 gtggaaagat attcgctacg aacttcgaag gcgggaactc gtcgccgtca tgacggtgtt
      601 cgcgttgctt gtcgtcgtga tattcgcgtt tgcgatcggc atacgctccg aatcgccggc
      661 ctccatggct gcctccgtcc tctgggtggc aacctcattc gccggaacgc tcggcctgaa
      721 ccgttccctt gcccttgagc gcgacgagca gtgcctcaaa gccctgttga tgagcccggc
      781 ggacagaacc tgggtgtttc ttgccaaagc aaccggcaac ctggtattcc ttctggcagc
      841 ccaggtgatt cttactcccc tcgttatcgt gctgttcggg gttgaagata ccggtcactg
      901 gtcgctgctc ggcgtcacgc ttctgctggg gtcagcaggg tttgtcggag taggcaccct
      961 gttcgccacg atggccacga gcacgcgcat gcgggattgg cttctcccga tcctcctgtt
     1021 tcccgtcgag gttccggtgc tcatcgccgc ggtgaaggcg accgcacatg tgttttccac
     1081 cgcgccgccc ggggagtcca ccatctggat cgccatactt gcggccttcg atctcatttt
     1141 cgtctcaatg tccattctct cgttcgggtt tgtggtcgag gaatgagtga ctgtcgaggt
     1201 gattacgcgc atgaatgagc ggaatggcac atcaggcgtt acgttggtcg tcggagcggg
     1261 ccttgcgggg ctgaccgcag cgtaccgctg gcgtcagcgc ggtgaggacg tcgtcctggc
     1321 cgaaaaagct ccgtacgtgg gcggcatggc gcggtcgcac taccggaacg ggttcacctt
     1381 tgatttcggt ccgcatttca tcatcgagaa aacgcccctg ctggatgagc ttttcgcccc
     1441 ggacgaactg gtcgcatttg gccccgaaat ccacatggtg ctcggaggcc ggtggattcg
     1501 gtatccgttc acaccctcct ccatgctgaa agtgccgctg ggcgtgcaga tggacttcct
     1561 gcggacacgc gctttgggcc cccgacccgg ggtggattac gggccgccgg actccttccg
     1621 gcgaaacatg agccgccagt tcggccgcgg gctgtacgac ttcttcttcc gcccgtacct
     1681 gacgaagaag ttcggcgggt gggatatcag cgatgttgtt cacagtgact ggtgggcgat
     1741 ggcaacccat acccgcggcg tggtcacgcg cttcgcaccg cctgcgccgc agaccgggca
     1801 tgcggtgggc gccctccgga agcttgcgcg gctgacgaaa gaactcccgt acgtgttcag
     1861 gaaactggcc ctgtggtacc ccaaagccgg gtttggggag ataccgaagc gcatcgccga
     1921 gcagttcact cacaacggcg gagtgattcg cttgaattgc cagatttccc agattcgccg
     1981 gaccgagaac cgtatcaccg gcgtggtagt ggacggagag gaaatcccgg tgcggcgcct
     2041 tatctggacg gcatctccca acctgcttgc gcgcatgctc ggaacgtcat ctctcgacat
     2101 gccgacattg cattcgatca tctatttcat cagcaccgat cgccggtacc gccggcgtgg
     2161 aactgaagta aggccggtgg accctgaatt cgcgttttac cgcgcctatt ttccggaaac
     2221 catctgggaa ggtcttacac ccggcggcag aagcgcggtg gttgccgaag tcggaaccac
     2281 gaatcgtgac gatctcgcaa aagcgggcga aaagtatgga gccataatcg acacctgcgt
     2341 ggctctgggg ctgtttcccc gcaacgcggt aaccgacatt ttccacgatg tgcaacccga
     2401 ttgctacccg gtgtatccac tcgattacga aactcacctt cagaagtact attctgagct
     2461 tacaccgatc cagaatctcc tgcttgccgg ccgcaacgcg cgattccagt actacaattc
     2521 tcatcaggtc attcgcgacg gggaacgcac ggtcgaagac gtgctgttct gatctccact
     2581 gcttcgatgc gtcgcccgcg tacgcgatct cactgcacat ggatcacttt tcgtgtattc
     2641 ccctggggtc aaaacccgag gtttcggtat attaagagag cgttaattcc acaagctgaa
     2701 atccgatatc ccggcgtgtg tccgcagttc cgcgcgcgag gcccggaacc aggcgcggcc
     2761 gcgtccgccg atcatgttcc ccgcgaggaa tcgcggggtt gatatacgga gcacattggt
     2821 ccttgagtgc agcatccatg gaagagcccg gctcttccac ccttgaagcc gccagccgaa
     2881 cttcccctga cgacatcatc gaccccagac acgagaagcg caactactgg ctcaccgtca
     2941 tcaacggcat catgacggga gggccaagca ccctgtttga cccgaacacg gttgtcgcgg
     3001 cgttcatcat ccacctcagc ggctctcagg ctcttgtcgg tctggtaagc ggactggtca
     3061 tgctgggcca tgtctggccg cagcttttcg ttgccaactg gatcgaaccg aaaccgaaaa
     3121 agctgtttgt gtaccacatc tcaacggtgt tgcgccttgc cagcctcact acccttggcg
     3181 tgctcatctg ggtcttccgt gatgacctgc cggggtggtt cgtctacgcc atgatcggcc
     3241 tgttgtttgc cttctggtct gcgggtgggt tcagtgccgt tgcctggtat gatatcctga
     3301 gcaagacggt gtacagctac aaacggcctg ttctttttgc ctggaggcag accggcgccg
     3361 gtatcatcgc cctgctcgcc ggcggcgtga tcacgtgggc gctcagcccc cgctcaggac
     3421 tgcgctaccc gctgaattac ctcttcctcc tctcgctcat gacggtcctc atcgccatcg
     3481 ggatcctctg cttcaacctg gtggtggaac ccgccgaaac gcatgccatc aaaaaacgcc
     3541 ggccattcag agagtatctg agactggggc cgcagatcat gcgcgaggac cacaattacc
     3601 gacgcctgtt ctgggggcag atcacctttt ccctcgccgt catgaacacc ccgtttctgg
     3661 tgccgttcct catcaaagat atccacgtgg atgacagcat catcggcatg ctcatggtgg
     3721 tggccgcaat cgcggacctc ctaatcaacg tgtactgggg gcagctcggt tcgcgaaaag
     3781 gcaaccgtgc agtcctcgtt cttgggagca ggctttccgc cttttccccg gcgctcgcgc
     3841 ttggtgcggt cctgttaccc cggttcgcgc ttttcggtgt tgacatcaga atcattcttg
     3901 ttgcgctgtc actggtgatg tcccgcatcg ctggcagcgg gctcggcgtg ggaaggatga
     3961 actatctcct cgacgttgct ccgctcggga tgcggccttc atacatgggg tttatgaaca
     4021 cgttctccgc cgtctcgatg atcattccaa tgaccgccgg cagcgcgatt caactggtcg
     4081 ggtactggcc ggtatttgcc ctcgcagtca tattcggtat cgtttgcgtc agagtcacca
     4141 cgaatctgga cgacgtgaaa ttcacatcgc acccgaacgg ataccgtccg gaagaaagct
     4201 gaattgcgtt ccgtgatgtc gtaatacgcg gactccggta gggagtctcc aggcccgaga
     4261 gcatcaggaa aggaaaaaac catggcgaaa atcaaagtgc tccagttcgc gggtggcgaa
     4321 atccacgact ggaagggtgt gggcgacgaa gttgaagcca ccctcaaaga agacagtgaa
     4381 tttgaagtga cgcgcgtgaa cgaggatctc aacgtgttca ccaccgacct cagcgcgtac
     4441 gatgtcgtgg tattccacta cactgtggga agcatcaccg acgcgcagcg ggatgggctg
     4501 ttcaacttcg tgaaaagcgg caaaggattc gtcggaatcc actcggccgc cgattccttc
     4561 cgtgattcgc cggagttccg ggatttcatc ggcggatact tcgttaccca cccgcgctac
     4621 cgcagctatc aggtgagcgt tctcgatgac aagcatccga tcaccgaggg gttggatgaa
     4681 ttcgtcgtga ccgacgagca gtacatcacc gattacgatc cccgtgtgaa cgtgctggct
     4741 accggcctgt acaagggcaa ggcgtaccct gtgatctgga cgaagtcgtt cggcaagggc
     4801 aaaatctgct acaacgccct cggccacgac gccaacgcct gcaaaaacat gtacttcaag
     4861 ctcacgctcg ttcgcggagt gaaatgggcc gccactacgg cgtgatccac acctttgacc
     4921 gagatcagcg ggacgggcca cgaggcctgt cccgtttttc tgttatctgt ccgatcccgc
     4981 gtgcacagga aacggccgta gcacgggcat cttgcccgtg ccggacgctg ccgtggcagt
     5041 tccagtaggt cgtgtttctg gattcccgcc ttcgcgggaa tgaccgcttt gtttcgcgcc
     5101 actttccggc attgcagcag caccctccgc ccgtcgtttt gcaccgcgag ttgccggggt
     5161 cgcttatccg ggcagctcgc gcggtgctcc cggcttcgtg cgcattgtac acctaccccg
     5221 gatgaacaaa tcacccgctt tctccatctt actaggagga agggagaggt gatgtgcggt
     5281 gtaaggtaac ccccgagaca ttcgcggagg cgcagtctgg cagccaccgc gcgtttgccc
     5341 gccttcatga ggaagtcgag ccgtgcgtgc gctggttcgc gtcgcgtctg atcggcctgc
     5401 cgtgggtgga tgacatcgtt cagaagacca tgaccgcgct ctacatcaac atcggcaaga
     5461 tcgcctgtgt tgaaagcctg ctgccgtatg tgttccgcgt cgtccgcaat ttctgttacg
     5521 aggatctgcg gcgcgagggc aggttcaatc tcccgccgcg ccaaaggcgc ggccagaacg
     5581 acgaagaacg tgatccgcgg catgtacgcg atcagaaccg tctcgccgac gaagcggccg
     5641 gcgtactcga gctggcgcat gaggttgcgc gggctttgaa cgagttgccg gaactgcagc
     5701 gggaagcact tatcctcttc gcggaaggtg acctgaccta cgctcagatc gcggaggcaa
     5761 tgcacagcga catcggaacc atcaagtcac gaattttcaa cgcgaggaag aacgtcatca
     5821 aacgcatcaa cccccgcgtc ctcgacgaac tggggattgg aaaggagatc accaatggcg
     5881 tctgaaaacg ataaactcag ggcactcatc cgcatgctcc cccctgcacg ggcattgcgg
     5941 caggaactca aggcggtcat cgccgcggaa acgtttgacg gcgtcggcag actggcggtc
     6001 aggaacttca ccggtctgct caactccgtc atgtcgctta ccgacgacgg gacttacctt
     6061 gacacgctca acgtcgagcc ttcccctaac ggtggtactg ccgacaaaga gcaggtgatg
     6121 gccgtcctct tctccctcag ccagttgatc gcctatctcg aagggcagat cggcttgccc
     6181 gcgagcggcg gcgaggaagt cagcacggac aaccgaagtt acatcaacaa cgacaacagc
     6241 gttgacatgc gcggtgcggt cttcagcccg gcatccggca atccactcat cgaccccgat
     6301 tcggtgggga agttgatcgg tggggaacgt ggccagaagg tagcagacat gctgcggtca
     6361 gtcttcgagg cgcgcgcggc gaagcaggac gaaggaaccg acgacgggga agaagaagct
     6421 tgaccgaggg gcggagcgca cccggatcgc cgggaaccaa cacgtgaggc agggacgaac
     6481 gccgaccgct cccacacccc gcaaaccaca aagcctgcgc ggtccctgag cttgtcgaag
     6541 ggcgcgcgga acattctgta gcaacacgac aacaaaaaga gcggggtctg cgccccgctc
     6601 tttcgcgttc tcgatctatc ccccgatcct caggccgccg gttggctgcc cactcgcctc
     6661 atcatccgct cataggtttc ccaccgggcg agcaccgcat tcacgtaccg cgcggtttct
     6721 gagccgcgga aataccccga cgacgcacgc cggtagaagc gctggtgggc cagaagctcc
     6781 attgccgctt ccacgttccc gcgccagcgg ttcgggtcac ggccgatttc ggccgccagc
     6841 ttgcgtgcgt cttccacgtg ccccagtcca acgttatacg cagcgagacc gaacgcgatc
     6901 gcgtcccgtt tctccagcga tccgaactga gcgatctggt cccggaggta cctcgtgccg
     6961 gcgtgaatgt tctgcaccgg gtccagaacg tcagccaccc cgagcgatgc tgcggtctcc
     7021 ggcatgatct gcatcagccc gacagcaccc atcgggcttt ccatgtcgtg ggcgaacccc
     7081 gattcctcat atgccaccgc ggccaccaga agccagtcca acccagcctc ttccgccgcg
     7141 agtttgatcg tatggtcata cggggatatt tgcccttcgc gaaggaatcg ggcgctcgca
     7201 gcacggtaac gggcggcgcg ctcgcctttg tcgaaatact tcgcgcggag ggtggcggtg
     7261 attcccgagg actgaactct gctcatgaag acgtccaccg catcgcggag ttggttggcg
     7321 ctcgggcgga cataccagcc gacaggctcg ttctggccta tccggggacc gatagtgatg
     7381 ttctggtggt agctccgttc gatcttcgcc aatggccacg gcgcgatgac ggcttccgct
     7441 tcgcctttcg aaagcgccgc aaggagcatc tcgtaatccc acccggacgg cgccggtgca
     7501 attgtaactg caccaccgag ctgcgtgttc aggtctacca gcgcgtaata tcctggcccg
     7561 ctcggaatcg cgtgcacggt gcgtcccgca agctcatcaa gagaagcaac agcagcgctg
     7621 ccgcgggtaa cgaccacgaa gtacgagcta tcgtacggca ggcaccggag cgccacggcg
     7681 tggttcggaa cagcggtcaa aaggcccggc gcgatgatgt cgcctttccc ctccataagc
     7741 cagggaatca tgtcttccgg ggtcgggggg atcaccatct gaaggtggac gtccagcatg
     7801 tcggcgaact ggcgcgccag ttcgtattcg agaccccgct catcaccgcg gaaaaggaag
     7861 tagcttgatg cggaatgctg ggtgataacg cgcaacgtat cacgtttctg aatatctgga
     7921 aggtcctcac gcaccgtggt tgcaagggat gcgtcttcca ttctcggcag ggagggcata
     7981 ttcacccacg ccgaagcctg gaacaacgtg aaaatcatga tgataaacca ataaaaatac
     8041 atatgttaag cgttcctttc gttactactg cgcaagcaga gcgacacgaa ttacgcgcag
     8101 tatcataagc aagagccatg ccacggcatt ttggggagag cagctaagtt cctgacgaaa
     8161 agtggtgcca gacgaggaaa aaacttcgcc ataccgcgaa agaaatcgga tgcatttccg
     8221 cgcacttagc gcttctgtgg ccggaaaggt ccagaaggct cccggagcgg aatcgtgcag
     8281 attctgcacg gttccgccac cctttcacct gcgtgatgtc gcttgagtga tgtcgcgcgc
     8341 gtgggcccgc ctgtcaccgg cttggtgcga caaccgaccg gccagaggaa aggctgcggg
     8401 agcggcactg gcgtattccg gttctcgtgg gagttgagac gcgtgaacgg atgccctcgt
     8461 tccatcctct cgggaccaga cgttcataac acgttttctg ttatttcact tgcgacactc
     8521 ccatcgcgaa gcggagccgt atttgccgcc ccgaatacat gcccgtacat tataaagtct
     8581 gggaaatgtt tcagggtgta cgcgttgcgg gccgcgaatc aacggagttg ccggaatgga
     8641 atccgcacag gacctgcacg ctcgaatcaa gctcgccgtg gatcatgtcc gggaggaaat
     8701 agcggacgcg gcggtgaaat ccgggcgctc tcccgatgag atcgcgctgc tcgcggttac
     8761 gaaaacgcgg tcgccggcgg aggttaacgc cgcgatcggg gcgggcgtga cgcttctcgg
     8821 cgagaaccgt gtacaggaag cggaagccaa gatcccgtat gtcgccggcg gagcggagtg
     8881 gcatctgatc gggcacctgc agaccaacaa ggcgcggctg gcggcgaggc tttttcggat
     8941 ggtgcaatca gttgacagtg tccgcctcgc ggagaaactg aacgacgcgg tggagtccgg
     9001 gcgttctctc gacgtccttg tcgaggtgaa cgtatccaac gaagcgtcca aattcggcgt
     9061 ggctcctgga tccgtcgggg agctggtgga aaaactggtc gagttgcccg cgcttcgcgt
     9121 tcgcggcttc atgacgatag gtgcgttcgt ggacgacgaa aggctcgtac gtcgcgggtt
     9181 ccgattgttg cgcgagatat ttgatgatac ccgccggcag ttcccgtcag gtcaagtgga
     9241 tatcctctcc atgggtatga ccggggattt tcggtgggcg attgcggaag gcagcacgat
     9301 ggtgcgaatt ggaacagctc tgttcggcca gcgtacgcaa aggggcgcgt gatgtttcag
     9361 tggcagttct tcctcaatgg agtgctcagg cttcttattt tgttgctgaa aatctacagc
     9421 atcatcatca tcgtgcgtgt cgtcgcgagc tggctcggcg cagacccgta caacgctatc
     9481 atgaacttcc tttcacgcat cacagacccc ttcttcgagg cgataaggcg gcgtctgccc
     9541 tcagcgatgt ggaacacggg actggatttt tccccgctga ttgcggtgct tttgattcag
     9601 gtcgtcacgc ttctgctgga gtcaattcgt ttctgagcca ccgcatcggc acatgggctg
     9661 gaggaaagca gcgatgaaca taacaccgct cgacattcga cggaacgagt tcacgcgagc
     9721 gctgcgcggg tacaaccgcg aggaggtgga cagcttcctc gtcatggttg ccgacgaggt
     9781 cgaaagcctc ataagcgaat atcgggacct cagccgaaag ctcgaggagc aggaaaaaca
     9841 gcttgaaaac ttccgcatga tcgagcggac gcttatggaa acgctgatta ctgcccagcg
     9901 cacgtcagac aacatgcgcg agacaacgca gcgcgaatgt gatgcgatca aacgggaagc
     9961 ggaactgaaa gcgcaggaaa ccatcgaaaa agcccgcctt gaggcggaac ggcttgccat
    10021 ggaagcacgg caacgggcgc acaacgttct cgaggatgcg cgcaccaaag cgcataacgc
    10081 aatcgaagcc gcgctcaagc aatcagacga gatctatcag gcagccaggg aagaagcaat
    10141 ggaaatccag actgccacga aagttatgat agaacggcgg gattcgttca tccttcagat
    10201 gagggcgttc ctcggcggac agttggaggc gctggaccgg ttcggccacg aggagctggc
    10261 tgcccctccc gcagtgcagg aacacgaagt tccgatcatc accgaagagg aagccgcggc
    10321 aattgcggcg ctcgacaagg aactgagcgc atttgcggcc gcaacggccc cgcccgaaga
    10381 atctgttccc gtatcggaac cacccgttcg cgaggaagct ccggccgagg aaccgcccga
    10441 agacggagca gaagtccttg tggggcggca gctaccccgc tcgcgggagg cctgagaagg
    10501 atgcaacgtg acgcagagcc gctacaccgt gtcacggtgc atgtgcaacc gcgggcatcg
    10561 aagaacgccg tgggagagat ggatgccgcg ggacggctca gagtggcggt tacggcggcg
    10621 ccggccgatg gccaggcgaa cgaggcggtt atcgcgcttc ttgcggatca cttccgcgta
    10681 ccgcgaggcg ccataacgtt ggagcgcggg aagaccgcgc gtacgaagat tttcgcactc
    10741 agaggcgcgc aaatcagcgc gccggcacat gacgggaatg caacatgaac acgccagaac
    10801 cgaaactgtc agagcagata cgtgaggccc ttgccgtcgt tcaggcgcgt tcctccctga
    10861 aaccatcggt cggcatcatt cttggcaccg gcctcgggca acttgcggac gagatcgccg
    10921 tggaaacggt gattccgtac ggcgagattc ctcacatgcc gctttcgacg gtggagactc
    10981 acgcgggcag gcttctgatc gggcacctcg gtggcaaacc ggtggtggcc atgcaggggc
    11041 gcttccacta ctacgaaggc tacacgatga aacaggtgac gttccccgtc cgcctcatgc
    11101 gggcgatggg agccgacacc ctgctcgtat ccaatgcgtg tggaggtatg aaccccgcat
    11161 gggaacccgg cgatatcatg atcatgaccg accacatcaa ccttctcggg gacaacccgc
    11221 ttatcgggcc caatgacgat tccctcggcc cccgtttccc cgatatgtcg gaaccgtaca
    11281 gccgcgaact gatcgaactg tgccgagcaa tcgcgcggga acacggcata ccggttcgcg
    11341 ccggggtgta cgtcgcagtc cccggtccca atctggaaac gcgggcggaa tacacgttcc
    11401 tgcggaaaat cggggctgat gtcgtcggca tgtccacggc tccggaagtc atcgtggcga
    11461 tccacggcgg catgcgtgtc cttggcctgt cggtcatcac cgatcgttgc ctgccggatt
    11521 cactggagcc cgcgaccatc gaacagatca tcgccatggc gaacaaagcg caacccggtc
    11581 tcaccacact catgcgtggc gtggtggaaa gactgtaatc cggaaccgca ttgggagcgc
    11641 ccgggatcgg ggcgacgccg cttcgaaggg accgtgggcc tcagccacag gtcctcaccg
    11701 ggttaggttc cgcgtgttcc gtttggatca tcctgtatca cagatttgtg gagaaagacg
    11761 aaagaactca tgtatcgtga cgtacccccg acattctcgc tgccagaact ggaacgccgc
    11821 atcctcgcat tctggaagga acacgacacc ttcaacaagc tccgccggaa gaacgagaac
    11881 ggtccccgct ggtcattcat agatggccct atcaccgcaa acaacctgat gggcgtgcac
    11941 cacgcgtggg ggcgcacgta taaggacgcc tatcagcgct accactccat gctcgggcag
    12001 cacctccgct accagaacgg cttcgattgc caggggttgt gggttgaggt tgaagtcgag
    12061 aaggaactcg gattcaagtc caaacgagat atcgaggagt acggcatcga caggtttgtc
    12121 gaagcgtgca aggcccgcgt gatcaagttc tcgaaacggc aggcggaaca gtccatccgt
    12181 ctcgggtact ggatggactg ggataactcg tactacacga tgtccgacga gaacaactac
    12241 accatctggc atttcctgaa aaagtgccat gaccgcggct tcatttatgc cgggcacgat
    12301 gtgatgccct ggtgcccgcg ttgcggcacg gccatcagcg atcaggaaat cgttacggaa
    12361 gggtatcagg aactcaccca cacgagcgta accgcgaaat ttccgctggt gggccggccg
    12421 ggtgaatccc tccttgtgtg gacaaccacg ccctggacac ttaccgcgaa cgtagccgcg
    12481 gcagttcatc cagagctgac ctaccttgcg gtgaaacagg gcgatgagac gttctacctc
    12541 gcgaaagcct gtgccgaacg aacgctgaaa aacgagtatg ccgtacttcg tgagcttccc
    12601 ggttccgaac tcctcggctg ggcgtactcg ggacccttcg acgaactccc acccgtggcg
    12661 ggtgtggaac accgggtgat cgcgtgggac gaagttaccg aagaggaagg caccggcatt
    12721 gtccacatcg cgcccggctg cggcaaagaa gacttcgccc tcggcaagca gttcagtctc
    12781 gcggtgattg cgccgataga cgagaacggg ctcttcgcgg aggacttcgg gccgttcgcc
    12841 ggaaaacatg cgtcgcacgt gccggaggac gtcttcgcgt cgctgaagaa caagggccgg
    12901 ctgtaccgga cgcagaagta ctcccaccgc tacccggtct gctggcgctg caaaaccgaa
    12961 ttgctcttcc gcctcgtgga cgaatggttc atagacatga gcaccctccg ttacgaaatc
    13021 atggaggtaa cgaagcgtat cacctggatg ccgtcgtacg ggctggacca cgaactggac
    13081 tggcttaaga acatgggtga ctggtgcatc tccaagaaac gtttctgggg cctggcgctg
    13141 cccatctacc cgtgtcagaa atgcggcaac gtggacgtca tcgggagcga aacggagttg
    13201 gagcaacggg ccgttgaagg atgggagcag ttcgcagggc actcaccgca ccgcccctgg
    13261 gtggatgcgg tgaaaatcgg gtgctcgaaa tgcgggtcgc cggtctcgcg gatcaaggat
    13321 gtgggtaacc cgtggctcga tgccggcatt gttccgtaca gcaccctgaa ttaccggcat
    13381 gaccgcgagt actggaacaa ctggtttccg gcggagttca tcacagagtc gttgcagggg
    13441 cagttccgca actggttcta cagcctcctc gccatgagca ctgtcatgga gaaccgcgag
    13501 cccttcaagc gcgttcttgg ccacgcgcag gtgcgcgatc tcaaaggcga ggagatgcac
    13561 aaaagcaagg gcaacgcgat ctggtttgac gacgccgccg agaagatggg cgcggagacc
    13621 atgcgttggc tctattgccg ccagaatccg tatacgaacc tgaatttcgg ctacgaggcg
    13681 gcggaggaac ctctgcggca tctcaccaca ctctggaaca cctacaagtt cttcgtaacg
    13741 tacgcgaaaa tagacggctt cgttccgggc agctccccgg tgccctacga ccggctcgat
    13801 cgaatggatc gctggattct cgcccggctg aaccaactga ttgccgacgc gcgccgggcg
    13861 ttcgacgggt tttccgtgat gaacttcgtg cacgcggcag agcgattcat tgacgacctt
    13921 tccaactggt acgtccgccg gtgccgggat agattccgac aggcggatca ggcgggagac
    13981 ccggccgcgt acgagacgtt gtacaccgtc ctctcgacga cggttcgact tctcgcgccc
    14041 gtgatcccga tgtttgcgga agagctgtac cagaacctcg ttgcgtggcg ggaaggggaa
    14101 cccgaaagcg tgcatctgac gccatacccg gaagagacgc ccgaatggga ggatgcggcc
    14161 ctcgttgccg atatggcgct ggtgcggaac gtggtggaaa tcgcgcttgc cgcccggaat
    14221 gccgcaaaaa tcaaagtccg gcagccgctt gccgtgatga cggtcgtcac cgatccgagc
    14281 gcaataaaaa cgctggaaat gaaccgtcag gtgatcgctg aggaacttaa cgtcaagcgg
    14341 atcgtcttta ctgattccgc cgaagggctc accacgcgcg tggcgaaacc ggtgttccgc
    14401 gcgctcgggc cacgctttgg aaaagacgcg aacgccgtgg ggaacatcct ccgcgcactc
    14461 gaccaggata cgctcgccag aatcgcggcg ggcgaaaccg tgaccattcc tgtcaacgga
    14521 aaacatgagc agatatccag tgaacttgtg gaggtcgtca cagccagccc gcagggcatg
    14581 tcggtaattt ctgacaagca ggttaccgtg gccctcgaca cgaacctgtc ggacgaactt
    14641 gtggacgaag ggtttgcgcg ggaaatcgtg aacaagatcc agaacatgcg taaggaagcc
    14701 gatttcaacg tctctgaccg gatcaccgtg ggctactccg tcagtccgcg ccttgcacgt
    14761 gcgatagatt cttgcagaac gtacatcatg aaggagactc ttgcgcgtga actggtgccg
    14821 ctcgaaggcg agtgggagca ccgctctgaa tggtcggtga acggagaacc cgcggaactt
    14881 ggagttcggc gcaccacgca atcaggcttg gcaaacggtg ggggaatgca agatggccgc
    14941 acttgacccg caggaactcg cgtatttcga gaagcttttg aaagaacggc gggcaaaggc
    15001 gctttccgct gcagaagagg gagaaagtgt ccgcccgaaa gaggcgccca aggaatcttc
    15061 gggagacctg agcgcttatt ccgttcacat gcccgatcag ggaacagatg ccatggagcg
    15121 cgagctggaa ttcctcctcg ccgctcgcga tgagaaatac atccgtcatc tggacgaggc
    15181 actccagcgc atcaaggacg gaaccttcgg catctgccgc acctgcaaca aaccgatatc
    15241 ccgtgcacgc ctggaagcgg taccgaatgc cacagaatgc attgcctgca aatcacagcg
    15301 agataagctc ccccgacgct gaaacgcgcc ccttctcatg gacaagggcg tcctgcgtgt
    15361 tcataggtcc cgcactggct tcgttgggtc tggatcgcat caccaaaatc gccgcggaac
    15421 gtcatctcac cttttccgtt ccggagaagg tgcttggcga atacgttcgc gtcgtcctct
    15481 ggttcaacga aggcgccgcg ttcggaatga cattcggtgg caaatgggcg catgtcatcc
    15541 tttccctgat tgcgatggtt gttgtcacct acctcgtctg gcggactccg cgcgaacact
    15601 ccctcatgct ttctggtttt gccctcattc tgggaggcgc ggcgggcaat ctgtgggata
    15661 ggctccttgc tggcaaagtc accgatttca tagacgtcgg cataggttcc ctgcggtggc
    15721 cgactttcaa cgtggcggac gccagcgtgg tggtcggggt gttcctcata ctccttacaa
    15781 gctggagaac cagccgcgcg gagcagaccg caaaagaaac tatggcgcgc cccgctcagt
    15841 ctgcggcaac ggaagggact cccgctccgt gaaaacgccc ctgcgcgcgg ccgattaccg
    15901 gctcctgctg agcctcggcc tcggtgtgct tttcaccgac tggttgacca aagcgcttgc
    15961 ggcgaatctc ctttccgccg gccgtcctgt ctctcttttc ggggattacg cgcgattgtc
    16021 cctgtcgcac aatccggggg ccgcgttcgg gatgcggttt ggcggtgcgg ctgtgcacct
    16081 gttggtcacc ctcgcggtga tcgcgttcat tgtggcaatt gccctgcaca ccgctccctc
    16141 cagaaggctt gagcaggccg gctacggcct tatccttggc ggcgccatcg gaaacctcgg
    16201 cgatcgcctc ctgctgggtc acgttattga ttttctcgat ctcggtatcg gcaacgtgcg
    16261 gtggtggacg ttcaacgtcg ccgacgcagc gcttaccgca ggcgtgggtc tgctcctcat
    16321 cgcatcattc cgctcgcgac gcaccgtgca ataattgcgc acgtggcatc cacgcgccgg
    16381 cccgtcgcca ttcctcaccc tcataaggat ccctccaaac gcacttccct tcgccctgga
    16441 gggacccgct tcgtcgggtc ctgcgccctg cgaggaggtt gggcgcgtgg gttttcccct
    16501 ggattcccgc cttcgcggga atgacaggca ctacacgagc attgcgtcat ttgtgcgtcg
    16561 tattcccatc ctcggggcgc tggcagatcc aaccttcaca gaatacccac gaggaaaagc
    16621 gccgtcattc ccgcgaaggc gggaatccag aacaccctcg tgaagagatt cttcgctttg
    16681 tccgcgtggc ggacgccgct cagaatgacg ggaaacgggg cgaacggtag atcagcggca
    16741 gggtgcaccg acggtcgggc cgggcccagt cgccgcgggg ctttggagtg cggaggcgcg
    16801 cccccgcttt gaaagcgcaa gcacgcttgc gcagttcaga gtcccttgcg ttgcggacgc
    16861 accgtttccc gccttgcgtt ccccacgcgc tcgaagtaat ctccttctac agcaccatgc
    16921 gactgaaacc ctgccgacaa ggaggcgccc atgccccgcc ccaacatcat cctcatcatg
    16981 tgcgaccaga tgcgagggga cgcgtttgga gccgacggca actcctccat cgaaaccccg
    17041 aatctggatt ttctcgcatc actgggcacg cggttctgtc acgcgtattc ggcggtgccg
    17101 tcgtgcctgc ccgcacgcgc catcctctgg accggacaga accagtggca caccggcgtg
    17161 ctcgggatgg gacgcgggca gggacagata ccgcacgact tcccgcacac ccttgccggt
    17221 gaactcgcca acgctggtta ccggacgcac ctcgtgggca aaggccattt ccatccccag
    17281 cgcgccccga tgggtttcca cgacatggaa ctggacgaat ccggtcgcat gcccaccagc
    17341 gagcatcgcc agtggttccg cactcatgcg ccggaaggcg taaccccgga cgaccatggt
    17401 gtggactgga attcctggca cagccggccc tggcacacgg acgagtacct ccaccccacc
    17461 gcgtggacga tgagccgcgc gctcaatttt ctcgacacat tcgactactc gcaacctttc
    17521 ttcctgaaca tttcgtttgc gcgaccccat tcaccgtacg ttccgccgcg attttactgg
    17581 gatttctacc acagccgcgg aaccccgccg ccttccgtgg gcgaatgggc aatcatgcac
    17641 gacgacccgc gcaccgcgct tgaccccaac gcctggcgcg gaaagataag ccccgatcgc
    17701 atccatcgcg cgcgaagcgg ctactacggt gagatttcgt tcatcgacac gcagatcggg
    17761 cgcctcatca aacgcttgaa tcgcggcaac gaccacgcca gtttcgccaa cacgtggatc
    17821 gtgttcacct cggaccacgg ggacatgcag ggcgaccacc acctctggcg caaaacctac
    17881 gcttacgaag gcagcgcccg cattccgttc gttatcgtcc cgccgagagc actggggagg
    17941 ccttcacggc aggtggccga cgaagtggta gaactccgcg acatcatgcc tacacttctg
    18001 gaaggtgcgg gagtagcact tccggggacc gtggacggac aaagcctcgt gcctttgctc
    18061 gatgcgcccg ccgccgaatg gcgcccgtac atccatggcg aacactgcac gtgctactcg
    18121 gaagagcagg agatgcagta tgtgaccgac ggccggcgca agttcatctg gctgccgcgt
    18181 atcaacgtcg agcagttctt cgatctcgaa tgcgatcccg gagaatgcca cgacctcatc
    18241 cacgatccgt tgcggcagac tgaaatcgct acctggcgcg gatatctcgt gaacgaactg
    18301 gcgaaacgcg actgcggctg ggtggtggac ggcaaactcc attgtccgcc cgacccgctc
    18361 gtttccccgt ggaaggacgt gcgctggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnntcg atcctctgga gcgggcaacg ccagtggcat accggcgtac tgggccaggg
    18541 acgcgggcag ggcccgatgc cgaatgattt cgcccacacg atcgccggcg aatttgccag
    18601 cgccggctac cgtacccacc tcgtcgggaa agggcacttc aacccacacc gcgcctccat
    18661 gggcttccag acgatggaac tggacgaatc aggccggatg cccgacagtg aacacaggca
    18721 atggttccgc acgcacgcac cggaaggcgt gacaccggac gaccacgggg tcgccttcaa
    18781 ctcatggcac gcgaggccgt ggcataccga agagcacctt cacccgaccg cgtggaccat
    18841 gagccggtcg ctcgcgtttc tcgaatcgca cgactccaca cagccctttt tcctcaacat
    18901 ctcgtttgcc cgaccccatt caccctatgt tccgccccgg tactacttcg acctctacca
    18961 taatcgcgag acgccgcccc ctcacgtagg agaatggtcc gcccgtaacg acgatccagt
    19021 caccgccgtg gatccaaacg cgtggcgcgg caaacagacg ccggaggcca ttcaccgggc
    19081 ccgtgccgcg tattacgggg aagtttcgtt catcgacacc cagatcggga gactcatgaa
    19141 ctggtgttcc cggtaccgcc gggccgcatg ggagaactcg tggattgttt tcatcgcgga
    19201 ccacggcgat atgcagggcg accaccatct ctggcgcaaa aaccacgcct acgagggaag
    19261 cgcacggata ccgttcattg tggtgcctcc gaaggagttc cgcggtgcga aacgggcggt
    19321 cgcgtacgaa gtggtggaac tgcgcgacat catgccgacg ctgttcgagg ccgccggcat
    19381 cccgattccc cccaccgcgg aaggccggag cctgctcccg ctccttgacg cccctgcgtc
    19441 cgactggcgg ccgtacatcc acggggaaca cagccagaac tactcgcccg aacaggagat
    19501 gcagtacgtc accgacggac gtcgcaagtt catctggctc ccgcgcatca acgtcgagca
    19561 gttcttcgac ctcgaatctg atccgggaga acgggtcaac ctgattgatg atccgtcacg
    19621 gcagagcgag attgccacgt ggcgggggta tctcgtgaag gaactggcgg aacgcgactg
    19681 cggctgggtg gtcgatggca gacttcactg cccgcccgat ccgctggtct ccccgtggaa
    19741 ggacaagcgc tggatgggaa cccggtagat cgccgcgaat gaacgctttg gggcgcaatg
    19801 catatagaag ggccgttttt ggaacatcgc tgcttgtgcg ccatattcgt cccatgggac
    19861 gaataatccc gtgcagcccc atcttggcag gcgggaatga cgcacacggc gcctggcgtg
    19921 tgcgccctgt cagaacctca ccggattccc gcattgcccc agaatccgtt ccgcgttgcg
    19981 gtggaagatg ttgtgccggt cattgtcgct gatgtgggcg tagaggatac atcccgccgc
    20041 ataatgcggg tcgaaccagg gcaaatcggt gccgaaaagc accttctccg atcccgcctt
    20101 gttcaccaac ttctccacta ccccgttcac tgcatacgcc gcggtcagtt cgaggtacac
    20161 atttgggtgt ttcctcgcca cgctgatcga cacgtcccac tcgccgtaac cggaatgccc
    20221 catcagaatg acaaggtccg ggtagcggtc ggccacctcg cccaccagtt caggcccatc
    20281 gaacttgctg ccgccccatg tatgcatcag caccggcatg cggtgctcct gcgcgtattt
    20341 cagtgcgggg acatatgcat cgccggtgat cgggtagcgg tggtagtcgg agaggaattt
    20401 gattccgaca aactccttgc gctccgccag tccgtcaagc tgccgcagca agacatccgg
    20461 ttcgttcgga ttgacgctcc agtatgcgcg gaaacgatcg gggaagcggc gcaccacatc
    20521 ggccatgcgg gcgttccccg tgtcgctgtt gacgagcagc gcttcgtgcg agctgcagac
    20581 gatgagccgc acacccgccc tgtccatcga accgaccatc tgttcggctg tcacgttcgg
    20641 gaaaaagatg ccgttgaatg gcccgaagtg accgtgcatg tcgataatcg ggcacgacac
    20701 gctttttccg agccggcgga attcctccat aagcggcgta gcatggttca tatcgtcacc
    20761 tcgtccagca ttcgggcgat gttgccgccc agaatgagtc ccttgtcggc ttcagggatg
    20821 tcggcatgga gtgcggtcaa cagcgcccca cccatggccc agtgggggta tcccgaaccg
    20881 tacacaagcc gttctgcgcc gtatttgccg cacagtgccg cgatgccgcc gtcaagctcg
    20941 taacgagaga cctcaacggt cagcgtggga aagcttcgca gcaacgggcg gaagagccgg
    21001 tcatcccccc acggcccggt gcccagcacg ataagccgca gcccgggtgt ctcactcagg
    21061 agtttttctg cgagcgccca gccgcccacg ccgccagaac tctcctccag cggcagcaac
    21121 agcggaatgc ggcactccac catggcgtcg agaagctcgc cgcaacccac ccggtcgagc
    21181 aaataccggt gtttcgaggg atatgccgcc agtgcacgga cgccgtaccg tttcatggaa
    21241 tcgaggaatt cggtccgtgt gccgaactct cccgtttcaa gcggcaggat ggcccacaca
    21301 ggcatcaacc gcgcgtgagg agcgagttcc agggccgcac ggacgttcat ctctgacggg
    21361 catccaccgc gaatcgcatc cgccgccacg agcgccctgt ccacaccggc gcggtcaagc
    21421 gcggcgatca actcctccgg cgtcctgcaa ggggcaagcg tcggtgtgta cactgcgccg
    21481 aacgacgcgt tcgcgtcaat gatggtcatg aaggttcctc ctgccggtgg gcgggaacgc
    21541 gccctccggg gaaaaatgct gcgcgttgac ttctcttcag cctttcgcag ccatcttgaa
    21601 cgcggcgatc ttttccgcga ctttctgctc cgtccaatcc gggtcgagcg agcagaacac
    21661 cgtgcggcgc agcaagtcca gcgagcgcgg gcaggcggtg tcgctgtaat ctgcgcgcag
    21721 gcccttgttc tgagggaagt tgaacgggtt catggcaggg tgatgcatga agcgcttctc
    21781 gcgtatgggt gtccactccg tgtacacgtg cttcccgtgg tctatgccga cgtaaccgcc
    21841 cacgcctggt gattgttgaa acgcacgggc tttggcctcg gtgtcgaact ggaacgccac
    21901 caccacgccg ctgtcgccgt cggggtcgtt actcggggcg aacctcaggc ccggctcccc
    21961 tgccagagcg gcttcgatgg tgcgtcgtac acgttgcagg tcggcgacga tgccgtccat
    22021 acgctggagc tgaattcgcg caatggcgcc catgacctcc gatgcgcggt actgctgagc
    22081 aatgaagatt ggctcagtga gttcgcctgc cttggggcgg aacgccgtgc cggagtcgtg
    22141 gaagattacg gcgcgttcag cgatcttctc gtcgttcgtt accagtattc caccttcgcc
    22201 gcaggagatg attttgtagt aattgaggct gaatgctccc gcgtcccccc acgtaccggt
    22261 ccgccgtcct ttgtacatgc ccccgttatt ctggcatgag tcttccagca ctttcacgct
    22321 gtgcctgcgg gcaatggcga ctatgcgctc catatctgcg gggcgcccga gcatatgaac
    22381 cgggatgact gccttcgtgt gcggcccgat cttgcgctcg aaatcatccg ggtcgagcgc
    22441 catggtctcg tcaatttctg caagcacagg gatcccgccg acggacaaca ccgccgacgc
    22501 agttgccagc caggtatatg cggggacaat cacctcgtcg ccagggccga tacccatccc
    22561 cgcaaggccg cagataagcg cggcagtgcc gcctccgctt accatgagcg cgtaatccgt
    22621 tccgatcttc tccgcccact ccttttcgaa acgatctacc tcttgcaggt gccccgcttt
    22681 cggatcgccc acgcggaaca actgcttcgc cagaatgacc ttcgcgactt cgtctacttc
    22741 ctcttgaccc atgcggtaca tagcgcggat tccttctcaa acgggggtgg tagcatgcaa
    22801 cacatataaa agttaacgaa atctgcgacc tgtagatgac tcccgaaaag ctgagcagtg
    22861 ggcagccatc ccgctcttgg ccctgcgcgg cagacagact accgttaatg gcggaacgcc
    22921 ggacagaaaa ccattcggga gggatacgta tgacaccggt tctcttctcc gtaagctacg
    22981 cggggctctg gggtcaggac acgctggacg tagagtcgtt catcgaacga gcggcgaagc
    23041 tgttctaccc ggccgtcgaa ctgatgggca agaggccgca tctctccgtc ctcgacgcga
    23101 gcccggaacg aatcaggcgt atccgggaac gagccaccgc caccaatgtg gagatcgtca
    23161 ccatcgcggc atacacagac ttcactgccg gccccgctcc ggaggtaccc ttaggtgaga
    23221 tgcaggtgct gtacgtgcgg gaacttgcgc gtatcgcccg ccttctcgat gcacacgtta
    23281 tccgcgtatt cacggggtac tccaccgatg accactcgta cgtcaccgac tggaagaaat
    23341 gcgtggccgg catacgtgaa gctgcaacgg tggcggccga tcagggtatt gtgctgggcg
    23401 tgcagaatca ccatgacacg gcaatttcta cggatgcgta cgtcgagttt ctcgacgagg
    23461 tgaaccaccc gaactgtaag gcgatgtacg acccctgggt gccggccctg ctgggagagg
    23521 acctgtacgc ctgcgcaaaa cgattggctc cccgcatggt gcaaacgaca ctcgccgact
    23581 acgtgcggat ccctcggtgg gcgtacgagc cgggtctggt caattacaga aagctgccgg
    23641 atatggtgcg cgcggtgccg cttggggagg ggttcattga cctgaatgcg ttcttccgcg
    23701 ggctgaaaga gggcgggttc gacggctatg ttgcgtacga aatgtgctcg ccccttcgtg
    23761 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagagggcg ggttcgacgg
    23881 ctatgttgcg tacgaaatgt gctcgcccct tcgtggcggc ggcgggtatg aaaacctcga
    23941 cctcgcagcc ggcaccagcc tgcggcgtat caccgcgctg atcggttaag aggccgttgg
    24001 tttgtgtcgg catgcccgtc gccattgagt gctgttcgtc attccgaacg aagtgaagaa
    24061 tctctccagg gagaacagga cccacaatga aacacgagac gacccaactc gaggttaccc
    24121 cgacgggcac cgtcggcatc tcctgcccat cctggcccgg atttgccgtg cgcgggctgt
    24181 cgccggcgct gcgtgtggac ggtgcggacc tcaaaccccg ttccgcgcac gtggcgacga
    24241 acacggatgg ctctaccgtc gtggagtacc ggttccgcga agggctctcg ttgacgtgcc
    24301 acgttgagcc gtggcggaat tgcggatacc gcttccacac cgtgcttcac aacaatggtg
    24361 gcacggcaac tgtgctgaac aacgtcgtgc tcctgggcac ggacggcgaa ggtttccgtg
    24421 tcgcctttgg cgcggcggat gacccggtcc gcgtgatgga gcagggcaac tactggggca
    24481 aagtgcggcc gttgagcgcg ccaaaagcgg tgcctgcaga aaacgcccag ggcgaaccga
    24541 acgaagcgcc gggcactacg cacggctcgt cggatttcgt gtgggtggca tacaatcagg
    24601 cctcccgcat ggcctttctc gccgggtttc tctcgtcgga gcgctggtcg gggcggatca
    24661 gcgtcgaaac ggcgcccgga ggcgaggtgc tccactgggg aatcggtttc gacggcgcgg
    24721 atgttcttct caaaccgcac agtgaaacac cgcttgagga cctgctgttc ctcgtgggcg
    24781 ccgacccgtg ggaactcctc gcatcgtatg ccggggaagt gcagacaatc cacaacccgc
    24841 agttccccgc ggtgcctccg gtctcgtggt gcagctggta tgcgtaccgc ctcggtgtgt
    24901 cggaggagcg gatcctcgaa aatgcccgca tcgccgcgca gcggctcaag cccctcgggc
    24961 tcggaatcat cgagctcgac ctcggctggg aggcgggcaa tctcccgagc acgttcgagg
    25021 agaacgagcg gttcccccac gggctcaagt ggctttctga ggaacttgcg aagcttgggt
    25081 tcgacatggg cgtctggaaa gcgccgttca ccatcagcga gttcgacccc atggcgaagg
    25141 agtaccccga gtacctgatt caaggcgaag acggaaaacc cgcgccgtac tgggactggt
    25201 tttgggagcc gcatggcaag gtgttcattc tggacctgac acaccccggt gcgcaggcgt
    25261 ggctgcggga gaagatgacg agcctcgccg aacgcggcgt gaagtatctg aaatcggact
    25321 tcatcggctg tgtcaccggc gaggcagcca agcgccggca caacccggac atcgccgcgg
    25381 ggggcgggct cgaagcggcg cgaatcggtg cccgtatcat cagggaatgc ctccccgacg
    25441 cgctgcagct caactgcggc ggcccggaga tgccgggaac cggtcactgg cctcttctct
    25501 acaccgttca ggacaccggc aatacgggct tcataggcca ctcgttccag cgcgataact
    25561 accaggctgc ggcggttcac ctgttcaaga actaccggtg gggcattctc cagccgtcgt
    25621 gcctctgcgt cggcgcgccc ggcacgctgg acgacgcacg gctccgcgcc accatcgcct
    25681 tcctcaccgg cgggcaggtg gacatctccg atacccttac cacgcttcca gaagaccgct
    25741 gggcggtgtt gaccgccaca ttgccgccgc tcggcatcac agcaaaggca gtggacctgt
    25801 tcgacccggt ctacgccgtg ccgttcgatt acgaagccat ctgccgtggc gaggcaagcg
    25861 gtgatgcaac gccgaaggag cacccgcccg gttccgtctg gaagctgcac gtgaagaccg
    25921 actgggacga gtgggacctc gttggcgtct tctcctactc gcccggagcg tccgccgaaa
    25981 caccggcgat ggaccgcttc tccatcccgt tctccatgct ggaaatccct ccggagagca
    26041 cacggtgggg gtacgaattc tggtcggggc agttcatcgg cacggtgccg ggaaaacgca
    26101 ccaacgatcg cgggtacgtc caccccggcg actatcagga cctcaccgtc ggggatgcgc
    26161 ccggcgtgct cgaaatcgcc ttcttcgggc cgtgcgtgaa actgcttgcg ctgcgagcac
    26221 aacgcccgca tccgtgggtg gtcggcacga gcttccacca gagctgcggc gcggagctgc
    26281 acaacgtcgc gtgggatgcg acaggcggcg tcctgagcgg tgaagtgcac cgtcccgtcg
    26341 gcgaaaccgg cttcgtggcc ctctccactg ccggcatggc cactgtttcg tgccgcgtgg
    26401 acggcatcgc ggtgcctcac cgcaccggcg cgaacggcag ccttatcctt cccgtcacca
    26461 tgaacgccga ccgcgccgag tggcagggaa ggtttgggtg aggaatgaac aggtggcagt
    26521 tgtgacactg gaacgcgcat attactccgc accgttcggc gatttcttcg gcgcaactga
    26581 tgatgagttg ctggggcacc tgtgccgccg gagtgaactc gacgtagagc aataccagag
    26641 gaatgcttgg atcgctgagt tcaacctact gcgccgccat cttttcggct tctctgggca
    26701 cattttgctg gagtacgcga ttcccagaat gggtcgtcgg gcagacgccg tgctgctcct
    26761 gcctcgattt gtcgtggtgt tagaattcaa ggtcggacaa gcaacctact ccccggacgg
    26821 actcgaccag gttttcgatt atgcgctcga tctcaagaat tttcacgaag gcagccacga
    26881 aaaatgggtc gtgccgattt tggtagcgac cgaagcaccg gaacaatcta gcacatttcg
    26941 ccggtttgag gatggtttgt tcgcgccagc gcgagccaac gccgcgaatt tggccgccgt
    27001 cctcagggaa gcggcgatta cgtcacacga ggcaccgccc gtggacgtcg cggcatggct
    27061 gaacgcgggg taccggccaa ccccgacgat cgtagaggct gcccaggctc tttaccgcgg
    27121 acacaacgtt gttgaaatag cccggagtga ggctggggcg gagaatttga cccgaaccgc
    27181 ggaaacgatt gccggcatcg cgaatcgatc ccgcgaacag gggatcaagt cgatttgttt
    27241 cgtcaccggg gttccaggag ctggaaagac cctcgcgggg ctgaatatcg ccaactcctg
    27301 gcacgacccg gagagtggcg aacacgccgt tttcctttcg gggaacgggc cgttggtgga
    27361 ggtgttgcgc gaggcgctaa tacgcgatga tgtgcaaagg gcgagtgcag cgggtggaaa
    27421 aaggacacgc tcggcttccc aatcaagagt caaagccttc gtgcagaacg tgcatcattt
    27481 ccgtgatgag gcgctggcaa cgttcgatcc tcccgttgag cgcgtggttg ttttcgatga
    27541 agcgcaacgt gcttgggatg cggcccaaac cgccagattc atgaagtcgc ggaagggcgt
    27601 gttgtcgcgc atgagtgccg cgaaggtgct ggtggg
//
LOCUS       DAOSOO010000016        27553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1744_length_27553_cov_5.579424, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000016 DAOSOO010000000
VERSION     DAOSOO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_27553_cov_5.579424"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..874)
                     /gene="ispH"
                     /locus_tag="PLT86_02320"
     CDS             complement(14..874)
                     /gene="ispH"
                     /locus_tag="PLT86_02320"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT35481.1"
                     /translation="MLKITRAKSAGFCFGVRRAVHTALETCQNTHKPVHMLGDIVHNE
                     RVVAQLREAGIRVVKSLDEVDDGVLLIRAHGAEPATYEEAKTRSLQIVDATCPLVKEI
                     HERARELHAAGYPLLVIGDHGHDEVKGIAAQVPDAIVVSTPEEVRSLGRKFRRIGVVA
                     QSTQDLDNVRAIVSELIPICIEIRFVNTICYPTAKHQMEIRAMPLENDVMIVVGSFTS
                     ANTKRMTQISRGLNPRTYQVTGADDLCADWFSGVNTVGIHAGASTPDSVVEEVIARIR
                     QLAGTPVEIV"
     gene            complement(1095..2048)
                     /locus_tag="PLT86_02325"
     CDS             complement(1095..2048)
                     /locus_tag="PLT86_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOT35482.1"
                     /translation="MRSPLSSALAFLGSILLVTGGTAESQEASFSGHRNAVRSVACSA
                     DGRMIASASLDKTVRVWQTDQSNAMRVLGEFPGSVLSVAVGTEGHLLVLGCADNTIRV
                     CTCTDGAVLRTIVTTSGPVYSVAISPDGHYVASSGNDGSVRIWQLEDGTLVRTFAGHA
                     APVWSVAFSPDGRYLLSASSDHKVGVWNVADGALIRTLEGHTAPVFDVTVTPDGRYVL
                     SGGVDRTIRVWNLSTGALMRTMRGHTGTVYGVAVNPGGTCVASSSADGTVRVWDISSG
                     LVQRTFSNETAIRDITFTGDGCNLVGGCDDKTVKIWSVRPS"
     gene            2412..3956
                     /gene="xylB"
                     /locus_tag="PLT86_02330"
     CDS             2412..3956
                     /gene="xylB"
                     /locus_tag="PLT86_02330"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT35483.1"
                     /translation="MGLLLGLDIGTTGVKALVIDDSGHTLGRHTIEYPLLIPKPGWAE
                     QDPATWWTATITAIGSVLAEAGVKGADIAGIGLSGQMHSSVFLDEHDEVIRPALLWCD
                     GRTTKQRYWITERVGGDAEMRRLVSNPPLEGFTLPKVIWLRDEEPANFRRLKTLLLPK
                     DYVRFRLTGEKLMEVADASGTAMFDVANRVWSEELMRKVDLPMDILVPVRESVAECGR
                     ISRDVAAMTGLREGIPVAGGGGDNACGAVGTGIVREGRVLASLGTSGVVFAHTDTVKV
                     DPDLRAHTNCHSVPNRWCLMGVMLSAGGSLRWLRDVVADSERASASQSGQDPYDVMAE
                     EAAKAPIGSEGLVFLPYLSGERTPHRDANARGAFVGLSLRHGREHLMRCVFEGITFGM
                     RDSLSIMRGLGLHISEIRATGGGARSAFWRQMQADVYGVPVVTVNASEGPAFGAALMG
                     GVCSGMFSDLADASDALVKIVSVSEPIPSNVSRYEEWYERYRALYPALKQEFANVATM
                     VEKHSQ"
     gene            3969..4658
                     /locus_tag="PLT86_02335"
     CDS             3969..4658
                     /locus_tag="PLT86_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35484.1"
                     /translation="MQATKARGKLTRREIKQDKLLIWFSRVSMVVQDNMWYIAGVILG
                     VVLLGTGYYIYRSWDARQTERGMAQVSGLEMLVRQEQYDRAITQADQIIGSFVGLPDR
                     MARLYKADALRGKGDYAGARAIYEKSKWDDEITSFHATKGLADCLGAEKQYERAGKLL
                     IEWVDDNKGSGFAPMALLEASTNLELALKYSEAQAALQRILDDYNESQLVGRARQRIR
                     MLEGAVQVTGS"
     gene            4849..5832
                     /gene="trpS"
                     /locus_tag="PLT86_02340"
     CDS             4849..5832
                     /gene="trpS"
                     /locus_tag="PLT86_02340"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT35485.1"
                     /translation="MRHKRLFSGIQPTGELHLGNYFGAITNWVRLQDQWDAIYCVVDY
                     HAMTVEYDSDTMRRRVLEVSALLIACGLDPAKCSLFVQSDVPEHTELMWVLSTLAPMG
                     RLQNMTQFKEKSEKNADNINLGLFAYPVLQAADILIYKAEAVPVGEDQLQHLELSREL
                     ARKFNTRFGETFPEPKQLMTEAPRILGLDGKSKMSKSLNNYIGLTETPEQVWEKLRPA
                     YTDPARLRKSDPGNPDICNIFTLHKMVSPKELVLEIDRACRKAEIGCIECKKHLAQNL
                     NNVLDPIRSRYSELEARPGEVLDVLAAGKERCRRTARETMDDVREKTGLRP"
     gene            5857..8115
                     /locus_tag="PLT86_02345"
     CDS             5857..8115
                     /locus_tag="PLT86_02345"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35486.1"
                     /translation="MEICPLFVENVNRWPLPSFPVTFGVPLAAGALLPGAPLVLRDSS
                     GKLFPVQSKSLAIHPDGSVRRLLVTAEPPVPGQKREAFSLESGGPEASETVGWNETRS
                     EWASIVVERQGDTLVIERGGQGWRLSVDGALTVEGFNHSLVGSFRIDTLSATDSGPVR
                     LRLDVAGAIWNGTTRLLDVDLVIQIFSRTPVLALDVIATNRTNREPVAVDHLRAKLER
                     LGGEYDPSVKVEVRNGPAEREVELPCAVRTDKLATSVFHGNEEKMRRASRLIGYGETG
                     FSRVGQSGEIVLAIRNFWENYPAGVAFDSDAIEIELFPRWAPPMQLHLGVGKTHEILV
                     ALLNPEDRECFPARGLVYSFGKPPLVQVPFEVRARAGTLSEYLPYMPETYPRMETNLF
                     DLFQNRIRGFGKLNWGDDYSPLYTNQKRGGEDVVWNNLEGDHPYHAFCQFARTGQFAY
                     FKDFHDSILHWMDVDFAERHADPRHEGALIAHTRRHVINGGTTPSHNWAEGFKEWYFM
                     TGDPRVERVLNQMADWFLRNKDRELRLPPYVRTWGWGLIQLAAIWEVTNRDELLELMQ
                     WMVEQLHTYFAEHEGLTQGFGETPGASSVNAFHSATVAIGAYHVFKISRNETARTLCL
                     EVTRSISDRRATSPEGIPTYVNGPEQNFPMQQAATLTLGAIGCAWELTRDKDLVRRGM
                     RMLEYCLDRGLQVDHMRIPGQFVEFEDGICLMPELVWPNCQLLSYQLRGILLFMKAAH
                     ECGYLKEVDYRF"
     gene            8313..8486
                     /locus_tag="PLT86_02350"
     CDS             8313..8486
                     /locus_tag="PLT86_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35487.1"
                     /translation="MSRGDLNIGRMEGKQILKPRVGKSSLARRRIGRVTSKARRRCAK
                     AELLFESWWMREA"
     gene            8699..9583
                     /gene="dapA"
                     /locus_tag="PLT86_02355"
     CDS             8699..9583
                     /gene="dapA"
                     /locus_tag="PLT86_02355"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT35488.1"
                     /translation="MLQLRGSFVALVTPFTSDDRVNHALLRELVEWHIEQGTDGLVPV
                     GTTGESPTLTYEEHEAVIETAVEAAAGRIPVVPGTGSNSTREAVHMTQHAKLVGADGA
                     LIVLPYYNRPSQAGLIAHIERLNEVGIPLVLYNIPSRTGINMEPETTEKLSYLDNVVG
                     IKEASGNINQMSEVVIRTRGRCAVMSGDDALTLPLLSVGGTGVISVVANLFPREMARL
                     VAAWEEGKREEALELHQRLFLLSKALLALETNPGPIKEAMSMVGYPVGHVRQPLAPLS
                     AANRERLRKLLVEAGLLS"
     gene            complement(9703..10122)
                     /locus_tag="PLT86_02360"
     CDS             complement(9703..10122)
                     /locus_tag="PLT86_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35489.1"
                     /translation="MAETVLVIDDDQDFLEATETLLDAMGFNVVTAASSSAAMEAVEK
                     VTPDVILLDVMMERADSGFTLAQRFKRKPELAKTPIIMVTGVSEHLGHRYSLDTPQER
                     QWIRADLLLEKPLRPEDLLGHIRQVLEQAHENSAAHH"
     gene            complement(10142..11866)
                     /locus_tag="PLT86_02365"
     CDS             complement(10142..11866)
                     /locus_tag="PLT86_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT35490.1"
                     /translation="MPTVDLTINGREITVEAGTTILQAARLAGFQIPTLCYLSGLSPT
                     GACRVCVVEVEGARTLVPSCAFPVADGMVVRTHTARVRAARTAIVQLLLANHPQECLT
                     CVRNGDCELQTLTRELNIREIPYQGERRLAPVDSSSPAIERDPEKCILCGRCVRVCQE
                     IQNVGAISFTKRGFQSEVAPAYDRQLDITSCVNCGQCIIQCPTGALREHSSVRKVWEW
                     LQDPNMHVVAQTAPAVRVGIGEMFGAAPGGIYTGQLVTALSNIGFDRVFDTNFAADLT
                     IVEEATELVRRVKNNEPLPMITSCSPGWIKYCEHYYPDLIPHLSTCRSPHEMLGALIK
                     SYYAEQYGIFPGNIAVVSIMPCTAKKFETERPELETNGFPSVDAVLTVREIARMIKTA
                     GIDFLNLPPRDFDNPLGTSTGAADIFASTGGVMEAALRTAAFYLTGKELANVDFRDVR
                     GLRGIKEATIKIGDMDVNVAVASGLANAKVLMDQIRKGESPYAFIEIMGCPGGCINGG
                     GMPQGSNVETVRARMRAIYTIDQTKVYRRSHQNPAIRKLYEEYLGEPGSERAHHLLHT
                     TYMARERS"
     gene            complement(12004..14004)
                     /gene="nuoF"
                     /locus_tag="PLT86_02370"
     CDS             complement(12004..14004)
                     /gene="nuoF"
                     /locus_tag="PLT86_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT35491.1"
                     /translation="MFKLLEPCCSKCWHSAEKRCPDVVYCALHGPQCHEDAACSALRK
                     ERKDRASGDLLECPVVHIGMGTCGMAAGAQAVYDAFLAEIAARNLSVDVMPVGCYGYC
                     EKEVLIDIQLPGKPRVFYANVTPQLAKRLVEHVLIEGKCASDIALGWLGNDGVVMPEG
                     SDLPRITDIPFFKPQLKIVLENCGLVDPDSLDSYIAANGFSALTKVLKKMKPEEVIAE
                     VQASGLRGRGGAGFPTGKKWEFANKAKGDRKFIICNADEGDPGAFMDRSVLESDPYRV
                     IEGLVIGAYAIGAHEGYIYIRAEYPLAVARLKRCIADAETAGLLGDNILGTGFDFHLK
                     LKMGAGAFVCGEETALIASIEGSRGMPRPRPPFPANSGLWGCPTNINNVETLANITTI
                     INRGGAWFAGIGTPGSKGTKVFALTGRINNTGLVEVPMGLPLREVIYEIGGGIPGGKR
                     FKAVQLGGPSGGCVPESLLDTPVDYESIKGTGAIMGSGGMVVMDESTCMVDVARYFMS
                     FVQGESCGKCIPCREGTKRMLEILERVAMVKPERNTDDQLRRFDGAMLLESLAHIVAD
                     TSLCGLGQTAPNPVLSTLKYFRDEYEAHLFDRKCPAGVCRGLLTYRIDTSKCTGCGLC
                     AAKCPQGAILGEKRQAHFIVSERCISCDACRQVCNFGAVLAE"
     gene            complement(14020..14577)
                     /gene="nuoE"
                     /locus_tag="PLT86_02375"
     CDS             complement(14020..14577)
                     /gene="nuoE"
                     /locus_tag="PLT86_02375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT35492.1"
                     /translation="MISTTEFASIARLDNGHKNREVDLAAVDEILDSYKPLAPHALIP
                     ILQDVQEHYGFLPEAALDRIGRALDISTTRIFGVATFYNQFRLHPLGKHVIRICRGTA
                     CHVKGSAALLDTLEMELNIKAGSTTKDGLFSIETVACIGACSIAPVAMVGDKFHGRLT
                     VKDIPRLVKSLKREDAKGAAEKAVA"
     gene            complement(14982..16457)
                     /gene="hydG"
                     /locus_tag="PLT86_02380"
     CDS             complement(14982..16457)
                     /gene="hydG"
                     /locus_tag="PLT86_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOT35493.1"
                     /translation="MKEIEVTQTPEPRPLSRPRPKQPAEQPAKASDIIDEAHIEGLLS
                     SSRSADKSEIREILDRAKELHGLTHEEVARLLMVEDPDLLAEMGHAARWVKEEIYGRR
                     LVFFAPLYMSNECVNNCLYCAFRRDNKEVPRATLTMDQIREEVEACERIGQKRLLLLV
                     GEFGPLSQIVDYLEQAIATVYATKKGPDEVRRVNINVPALDVEHFKRLKAARIGTYQL
                     FQETYRRSTFERAHPGGPKSRYDYHLQAMDRAQLGGVDDVGIGALLGLDDYRFEVLAM
                     VMHAEHLDRTFGTGPHTISVPRINPAENAPWAMNPPHPLTDEEFKKVVTILRLAVPYT
                     GLILSTRENPSLRREVFALGISQLSAGSRTQPGGYKADDPEELAQFSLGDHRPLDEVL
                     REVASLGYIPSFCTSCYRSGRTGMDFMDLAKPGLIKQYCLPNALSTFAEYLEDYASPE
                     TKEIGIKAIEEHIKGIDSEALRRTSRALVDRVRKGERDVMV"
     gene            16802..18475
                     /gene="murJ"
                     /locus_tag="PLT86_02385"
     CDS             16802..18475
                     /gene="murJ"
                     /locus_tag="PLT86_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOT35494.1"
                     /translation="MAESPSVRISPPNDDSFPSPQRKIFGYLSFQVLGTLVSRAAGFV
                     RELVTAVVFGAGASADAFIAALTIPTLFRDVLGEDVVERSFMPGIREHLARKNHADAW
                     RLASVSLNWMLVALVLGMGVLWVAAPWLVDLVAAGIHQRSVSEGTVADVIAMTRILIP
                     FLVFIALGAYVGGVLYYGFDFHLVFSLAPTMLSVGVIAIVLFSSQDIGIYALAWGFTV
                     GAVLQFAVQVPFLGSQKIRSTNPEYTLSLRLNPAERSRISRETAHVALQSLLTKTTEI
                     VDRRVASFLVEGSISSLWFAARLVQLPFAIIGIAIGRAVAPYLSEKVGTGDLREFKNA
                     VLIGYRYNLVLVLPVIGLFTVLAHPFVRLIYERGNFGAHDTSMTASAFWAYALGLLGL
                     SLYTLGTRICSALEHNRVATYTAVLGAGLNVWLNYVLSATFLRHAGLALATSIAFSVN
                     AALLLMWVHRYLAQCGQGFRTRELLVPFLRVLANTAGAVLAAWVVLAFFVPESWFVTR
                     SFTASVGAFCVPFAGGFLVYFVLAVINPVAEIVPVKRWMKRSVIRFGNR"
     gene            18528..20627
                     /locus_tag="PLT86_02390"
     CDS             18528..20627
                     /locus_tag="PLT86_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT35495.1"
                     /translation="MGHRFILTGGGTGGHVTPAIALGEELRAREPDAQFLYVGTRTGK
                     EAEIVPRYGFPIAYVQSHQWSFRRKWHVLRFIWRIACGTIKACHLIIKYRPDAVIGTG
                     GYVAAPVVFANVLLRRIRLSRALTIIHEQNTVPGKLNALVGKVADLVLLSFPTASARF
                     PRAVCVGYPVRPEIAPADRTEAKRRLGIPQETTVVLAFGGSMGARTINRAVVDALPFL
                     RDRKDLHIVHGTGRKLSSYDPVADCESRMQRNGLTYESLSGWYEAHGFIDRIGEYYAA
                     ADVVVCRSGAGTLAELCACGRAALLIPKANLPGDHQVLNALEMARRGAADILFEDAVT
                     TNGAPEEYVAGEELARKIVELLEDKPRLGKMESNALASARPEATKVSVGVILSLLEGR
                     SPDGLLLEFAAREPVVGDAHLDTRFGRVAALSGEALRAYAETAVSSISRSLNCASGTP
                     SDQEDALVRALDADEMVAYLRYRAATHLTSKNWRMRNAGVKMVGILRQRDKLPVLAGF
                     VKDRAPASRMRRLFGGDFETNGFIRRNSMASLIRLGVASPLVREVILWGLEDPYFEVR
                     SQSARAVQAFRASLENDQEIVERLLNCAKDKYFEVAVEAVKAMGSVASAETALEVFRS
                     LSQHENWRVRQAVVEACCALLDRGQDVPKAFLSEILAGMMVTSTGFCPSFSLKLAVAK
                     LGERIREPDSTAGDRGK"
     gene            20630..21781
                     /gene="mraY"
                     /locus_tag="PLT86_02395"
     CDS             20630..21781
                     /gene="mraY"
                     /locus_tag="PLT86_02395"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT35496.1"
                     /translation="MLYHLASEAFQWYRQAPVSFPVLGSDVLLLTRFLMYLSVRAILA
                     ILTSFVVTMLVGRRLIRFLHAHHVRDTVKDFGTISATDKRGTPTMGGLIIITSLVVPV
                     LLWCDLTNRFVQIALAATAWFGALGVVDDYLKVRPYRGRIRGLGEAQKLALQGLFGLG
                     LGIVYLTPAISPVPQEVAGSFYVPFYKYAVLHDIGWWYLPFIVFVTLLISNSVNLTDG
                     MDGLAIMPANFAVLVYGVIAYAMGNLVYAEYLRYDYLHGSGELLVFCAALFGAGLGFL
                     WYNAYPAEIIMGDTGSMALGGILASLAIMLKQEVLFLIVGGVFVAETLSSFIQQKIGE
                     NFLGRRIFYRAPLHHQMEYRGLAETKVVIRLWIIAGLLAMVGLASMKLR"
     gene            22054..23088
                     /gene="ltaE"
                     /locus_tag="PLT86_02400"
     CDS             22054..23088
                     /gene="ltaE"
                     /locus_tag="PLT86_02400"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOT35497.1"
                     /translation="MIDIRSDTVTKPTAAMRAFMANAEVGDDVYREDPMVRALEEETA
                     ALLGKEAALYVPSGTMSNQIGVWVNTHEGDEVICERDCHIFNYEAGAPALLSRVLMKT
                     LQGARGVISAAQVEEAVNPANIHAAATTLVCLENTHNKAGGTIYPREEMERISDLCKR
                     GDMRLHLDGARLWNASAATGIPERDYAELADTVSVCFSKGLGAPVGSALCGSQEVIER
                     ARRKRKILGGGMRQAGIIAAGALYALRNHRVRLVEDHANARCLAEAINELPGIKVDLE
                     GVQTNIVMIEITGNATAQQVSAMMKEEGVLINAMRPKVLRAVTHLDVSARDIEKAIEA
                     FARISRRIRS"
     gene            23106..23978
                     /locus_tag="PLT86_02405"
     CDS             23106..23978
                     /locus_tag="PLT86_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35498.1"
                     /translation="MKIAFHQITSGSKRDLKETFKAYAEAGWRHFEVNLWETEAFVTQ
                     HGAKSLAALAKDFGLHCVGATGLSIPAFQGGEAHEAATKQMEIHAQVMNALECTTIVV
                     GGDVPKDFPPRAQNSTEKALSARDATYRNALKEFAMAVERVAETAAKYDVTLALEVNW
                     CGLARSFRTMAELVMMVDRDNVGATWDPAHFYSTASRLSDLDRLRGKIVHAHLNDIRD
                     CFMEAMDINGDRVLPGDGVLPLVEWTDKIHSLGYRGWHCVELFSDDLWAEPLHEIATR
                     AKKACERVWPDAEF"
     gene            24062..25654
                     /locus_tag="PLT86_02410"
     CDS             24062..25654
                     /locus_tag="PLT86_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOT35499.1"
                     /translation="MEMNEQEQKIAAAREMRERLLRDRYRPGYHFAIPEDIGMPGDPN
                     GAFWANGRYHLMYLYNRRGAKAWGNAAFCWGHISSADLVHWRHHPDALLPGDGDGGCF
                     SGGAFVDEDGTAYLTFWRLPLADGKTEGTGIGIARSRDRNYDTWEKMPVPTLDGTEFG
                     IRETRDSEGKAYPLCNADPSNIWKKNGTYYLQTGNLLVLRKMEKSDDASAKWKGDWVD
                     LFRSRDLVDWEYVHRFYARNPTNRWTDESEDDMCPSFLPLPSSPSGGFPSGKYLQLFI
                     SHNKGCQYYIGTYDEGSDLFLPETHGRMTWVDNTFFAPEALIDDKGRQIMWAWLLDNA
                     PSEPEQQILTGWSGVYCLPRTLWLGEDGMLRMAPVPELSVLRYNPRVFDDVNLHPGSE
                     KTLEVANGESAEINLSIESLTATQAGLKVRTSPHDEETTLLYCDFEHNKLVFDSTRSG
                     NAGRKVKEEAPFECRKGERIDLRVFIDRAVVEVFAGDRQAIARRVYPVRDDSTGVRLF
                     CVGGEVAFTMIRTWEMMPSNAW"
     gene            25708..27282
                     /locus_tag="PLT86_02415"
     CDS             25708..27282
                     /locus_tag="PLT86_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOT35500.1"
                     /translation="MELHARTNCIVEIPFRSAAVYRNPYADVTLDVAFSNSRGAKIIV
                     PAFWAGGGTWKVRFSAPSAGTWKFQSICDNRSDIGLHGRTGAISVQRYRGSNPVLKHG
                     RVRVSPNGRYLEHQDGTPFFWLGDTWWMGLCTRVDWPQGFKELTADRVAKGFSVVQII
                     AGPYPDMDAFDPRARNEAGFAFERGFTSVNPSYYDHADLKIGHLVESGLVPCIVGMWG
                     YYLPEIGVEQIKRFWRYLIARYGAYPVVWCAAGEGTMPWYLSKTPAEDAQKQKSGWTD
                     VCRYIRSTDGFGNVLTIHPTRYGHEQVEDRAVLDLNMLQTGHSGYESVGPTIESVRTA
                     YALNPPMPVLDSEVNYEGILGKSWQDVQRLCFWHSILNGACGHTYGANGIWQCSTKAE
                     PYGASPHGRSWGNTPWQEAMKLPGSTQLGLARKFLLRIDWHQMEPHPEWTQDGASAAG
                     IPKRLRVIYSPMVWDPPAIKELEPDVKYHARFFDPVTGAEYPIGPVDPAPDRTWTPPV
                     FPEMHDWVIVLKAVTG"
BASE COUNT         5692 a         7729 c         8260 g         5872 t
ORIGIN      
        1 cagagccgca acgtcatacg atttccaccg gcgtccctgc caattgcctg attcgagcaa
       61 tcacttcttc cacgacactg tcaggggtag aagcccccgc gtggatgcca actgtgttga
      121 cacccgaaaa ccagtcggca cagagatcgt ccgctcccgt tacctgatag gttcgagggt
      181 tcaaccctct ggatatctgc gtcatccgct tcgtgtttgc gctggtaaag gaaccgacta
      241 cgatcatgac gtcgttctcc agcggcatcg ctcggatttc catctggtgc ttcgcagtcg
      301 ggtaacagat cgtgttcacg aaccggattt cgatacagat cgggatcagc tcgctgacga
      361 ttgcgcgcac attgtcaagg tcctgcgttg attgagctac cacgccgata cgacggaact
      421 tccgcccgag tgacctgacc tcttcgggag tcgaaaccac aatggcgtcg ggcacctgcg
      481 cggcgatccc tttgacctca tcatgaccgt gatcgccaat tacgagaagg ggataaccgg
      541 cggcatgaag ttcgcgcgct cgctcgtgga tctccttcac aagtgggcag gtagcatcga
      601 cgatctgcag gctacgtgtt ttcgcttcct cgtaggttgc aggttctgct ccgtgcgccc
      661 ggatgagaag gacaccgtca tccacctcat ccagactttt cacgacccgg attcccgcct
      721 cacgcaattg cgccaccact cgttcgttat ggacgatatc gcccagcatg tgaaccggct
      781 tgtgcgtgtt ctggcaggtc tcgagggccg tatgcaccgc ccgtctcaca ccgaaacaaa
      841 aaccagcgct cttcgctcgg gttattttca acatccattc ccttcttcga aggaaccgcg
      901 ttccacaaga ggtgtgggtt acgacactcc cggatgtgat cgcaaacttc atctgcaggt
      961 gcgaagtatc ctacagaaaa ggtacgcacc gcccttcttc ggaagaagaa aaccctcaat
     1021 aagcgtgagc ggttcccgag agaaccgctc acaccggctg ataatactgt gacaccgttg
     1081 agtttcggaa taatctagga tggacgaaca gaccagatct tcacggtttt gtcgtcgcac
     1141 ccaccgacga gattgcagcc atcgccggtg aacgttatgt cgcggatcgc cgtttcgttt
     1201 gagaacgtcc gctgcacgag cccggacgaa atgtcccaga ctcgcaccgt tccatccgcg
     1261 ctgctggaag caacacaggt tccacccgga ttcacggcaa ctccgtagac tgtccccgtg
     1321 tggcctctca tagtccgcat gagcgcaccc gtcgacaggt tccacacgcg gatcgtcctg
     1381 tcgactcctc cgcttagaac gtaccgcccg tccggcgtca ccgtcacatc aaacaccggg
     1441 gcggtgtgcc cttccagcgt acgaatcagg gcaccgtcgg ccacgttcca tacccctacc
     1501 ttgtggtcgc tgcttgccga caggaggtac ctgccatcgg gagaaaacgc gacagaccat
     1561 acaggagccg cgtgtcccgc aaaggttcgg accagtgttc cgtcttcaag ttgccagatt
     1621 cgaacagagc cgtcattccc gctcgaagcg acgtaatggc catccgggct gatggcaacg
     1681 gagtacacag gaccggatgt cgtgactatg gtcctcagca ctgccccgtc cgtgcaggtg
     1741 catacgcgaa ttgtgttgtc ggcgcacccg agcaccagca ggtgaccttc ggtaccgaca
     1801 gcgacggaga ggacagatcc ggggaactcg ccgagaacgc gcatcgcgtt tgactggtcg
     1861 gtttgccaga cgcgtactgt cttgtcgagg ctcgcggagg cgatcatgcg tccgtcggcg
     1921 ctgcatgcga ccgacctgac tgcgttgcgg tgcccgctga acgacgcttc ctggctttct
     1981 gccgtcccgc cggtcaccag gaggatcgat ccgaggaatg cgagggcgga tgacaatggg
     2041 ctgcgcatcg tgctgctcct tcttctccgg cgatgctttt ggcatgccga tggttcgtga
     2101 agggggattc ccatcgggtt ttccaacggg aatccgggga agcacgccgg ggttcccggg
     2161 gtgcttttcc tgtgcgaaga atgtcgcgtt tgttgtctct gattcaaaga cgcttcgtcg
     2221 acattccggt tccccgcccg aacgtgttga aagttcagac gccggatcct cctgatttgt
     2281 tacacgttcg cttttttaac cgtgccattc cgcgcccggc cagtcgcgtt gactcccggc
     2341 gaccgtcctg cgtacctttg aattccttgc tccggccaac gcgaaaacgt ttcgcaatga
     2401 ggaatcttcc gatggggttg cttctcggtc tggatatcgg caccacgggt gtgaaggcgc
     2461 ttgttatcga cgattcggga cacacgctcg gacgtcacac cattgagtac ccactcctta
     2521 ttccgaagcc cgggtgggct gagcaggacc cggcgacctg gtggaccgct actattacgg
     2581 cgatcggttc cgtacttgca gaagcgggcg tcaaaggcgc cgatatcgcg ggcatcggat
     2641 tatccggcca gatgcatagc tcggtgttcc ttgacgaaca cgacgaagtg atccggccgg
     2701 cccttctatg gtgcgacggg agaacaacga aacagcgcta ctggatcacc gagagagtcg
     2761 gtggtgatgc agagatgcgg cggcttgttt ccaacccccc attggaaggg ttcacgctgc
     2821 cgaaagtcat ctggctgcgc gatgaggaac cggcaaattt ccggcggctc aaaacgcttc
     2881 ttcttcccaa ggattacgtt cgcttccggc ttaccggcga gaagctcatg gaggttgcgg
     2941 acgcctcggg aaccgcgatg ttcgacgtcg cgaacagggt atggtcggaa gaactgatga
     3001 ggaaagtgga tcttccgatg gatatcctcg ttccggtacg cgagtcggtt gccgagtgcg
     3061 gcaggatctc gcgtgatgtg gccgcaatga ctggccttcg agagggcatt cccgtcgcgg
     3121 gaggcggagg cgataacgcg tgcggcgcgg tggggaccgg gattgttcga gagggtcgcg
     3181 ttctggcgag tctgggcacg tcgggagtcg tgtttgctca cactgacacc gtcaaggttg
     3241 atcctgacct ccgcgcgcac accaactgcc acagtgtgcc gaaccggtgg tgcttgatgg
     3301 gggtgatgtt gtccgcgggg ggtagcctgc ggtggcttcg tgatgtggtt gcagacagcg
     3361 aacgcgcttc ggcgagccag tctgggcaag acccgtacga cgtgatggcc gaagaggcag
     3421 caaaggcacc gattggcagc gaggggctcg tttttcttcc ctacctgagt ggagaacgca
     3481 cccctcacag agatgcgaat gcccgcggtg cgtttgtcgg gctcagtttg cggcacggaa
     3541 gggaacacct catgcgctgc gtcttcgaag gaataacctt cggtatgcgc gattcgctct
     3601 cgatcatgcg cggactggga ttgcacatct cggagattcg agctacgggt ggcggggcca
     3661 ggagcgcgtt ctggcgtcag atgcaggcgg atgtgtacgg tgtgccggtg gtcacggtga
     3721 atgcgagcga aggtcccgcg tttggcgctg cgcttatggg cggcgtatgc tcgggaatgt
     3781 tcagtgatct cgcggatgcg tccgacgcgc tggtgaagat cgtttcagtt tcggaaccga
     3841 ttccttcgaa cgtttcgcgg tacgaggaat ggtacgaacg ataccgggcg ttgtatcccg
     3901 cactcaagca ggaatttgcc aacgttgcca ccatggtgga gaagcattcc caatagaagg
     3961 agttatgcgt gcaagcaaca aaagctcgcg ggaaattgac acggcgggaa atcaagcagg
     4021 acaagctgct catctggttt tcccgtgttt caatggtcgt ccaggataac atgtggtaca
     4081 tcgccggagt cattctgggc gtcgttcttc tgggaaccgg gtactacatc tatcgctcat
     4141 gggacgcccg gcagactgag cgcgggatgg ctcaggtgtc gggactggag atgctcgtcc
     4201 gccaggagca gtacgatcgt gccatcaccc aggccgacca gataatcggg agtttcgtgg
     4261 gtttgccgga ccgaatggca cgcctctaca aagcggatgc attgcgtggc aaaggggact
     4321 acgccggtgc acgggcgatc tacgagaaat cgaaatggga tgatgaaata acgagcttcc
     4381 acgcaacgaa gggactcgcg gactgccttg gtgcggaaaa gcaatacgaa cgcgccggga
     4441 agcttctcat cgaatgggta gacgacaaca agggatcggg attcgccccc atggcccttt
     4501 tggaggcgtc cacgaacctg gagcttgccc tcaaatacag cgaagctcag gctgcgttgc
     4561 agaggattct ggacgactac aacgaatcgc agcttgtcgg gcgtgcgcgg cagagaatcc
     4621 gaatgctgga gggggcagtg caggtaaccg gcagttgagg cgtttttgtg caaaaagcgt
     4681 ccgcatagca ttgtgatttc tccgcgcgga cggatgtgat gcaggagtgg tttggaggag
     4741 ccgtgagctt ccgcttgcgg ctcctgtcgc ttgtgtgggt gattatcgca agccggtgca
     4801 cgtgcgcggc tcaaaacgaa tgatccaaaa acgggactgg gttttctcat gaggcataag
     4861 cggttattca gtggcatcca gcccactggc gagcttcatc tcggcaacta tttcggtgca
     4921 ataacgaact gggtgcggtt gcaggatcag tgggacgcca tctactgcgt tgttgattac
     4981 cacgccatga cagtggagta cgattccgat acgatgcgtc gccgcgtgtt ggaggtttca
     5041 gcgcttctta tcgcctgcgg cctcgacccg gcgaagtgct cgcttttcgt ccaatcggac
     5101 gtgccggaac acacggaact catgtgggtt ctctcgactc ttgctccgat gggcaggctt
     5161 cagaacatga cccagttcaa ggaaaagagc gagaagaatg ccgacaacat caacctcggg
     5221 ctttttgcct accctgtgct gcaagccgcg gacatactca tttacaaagc ggaggcagtg
     5281 cccgtgggtg aagatcagtt gcagcacctc gagttgagca gggaactcgc caggaagttc
     5341 aacacgcggt tcggcgagac cttcccggag ccgaagcagc ttatgacgga agcgccgcgg
     5401 attctcggcc ttgacgggaa gagcaaaatg agcaagagcc tgaacaacta catcgggctc
     5461 acagaaactc ctgaacaggt gtgggaaaaa ctgaggccgg catatacgga tcccgcacgg
     5521 cttcgcaaaa gcgatccggg aaacccggac atatgtaaca tcttcaccct tcacaaaatg
     5581 gtgtctccga aagaactcgt tctcgagatc gaccgcgcat gccgaaaggc ggagatcggg
     5641 tgcatcgaat gcaaaaaaca tctcgcgcag aacctgaaca acgttctcga cccgatcaga
     5701 agccgatatt cagaactgga ggcgcgtccg ggcgaggtgc tggacgtcct cgcggcggga
     5761 aaggaacgct gcagacgaac ggcgcgcgag accatggatg acgtgcgcga aaagacagga
     5821 ttgagaccgt gactgcgtga aaaccggagg gaggcgatgg aaatctgtcc cctgttcgtg
     5881 gaaaatgtga accgttggcc ccttccatcc ttcccggtca ccttcggagt accgttggcg
     5941 gccggcgctc tgttgccggg cgctccgctg gttctgcgag actcttccgg caagctcttc
     6001 cccgttcaat cgaaatccct tgcaatccac cccgatggct ccgtgcgccg tctgctcgtt
     6061 acggcagagc ctcccgttcc aggccagaaa cgggaggcat tcagtctgga aagcggtgga
     6121 ccggaagcct ccgaaaccgt cggctggaat gaaacgcggt ccgaatgggc gagcatcgtt
     6181 gtcgaaaggc aaggagatac ccttgtcata gagaggggtg ggcagggctg gcgcttgtcg
     6241 gtcgatgggg cactcacggt ggagggattc aaccattccc tggttggatc attccgaatt
     6301 gacacgctat ccgccacgga ctctggtccc gtccgattgc ggctggatgt tgcaggtgcc
     6361 atctggaatg gcaccacgcg gcttctggac gtggacctgg ttatccagat cttttcccgc
     6421 accccggttc tggctcttga cgtcatcgcg acgaatcgca ccaatcgtga accggtcgct
     6481 gttgaccacc tgcgcgcaaa acttgaaaga ttgggtggcg aatacgaccc gagcgtgaag
     6541 gtagaggtgc gcaacgggcc agccgaaagg gaagtggaac ttccgtgtgc cgtgcgaacg
     6601 gacaagcttg cgaccagcgt ttttcacggc aacgaggaaa agatgcgtcg ggcgagtcgt
     6661 ttgatcgggt atggtgaaac cgggttctca cgggtaggcc aatcgggaga gatcgtgctg
     6721 gcgattcgca atttctggga gaactacccg gccggggtgg catttgattc agatgccatc
     6781 gaaatcgaat tgtttccgcg ttgggccccg ccaatgcagc tccatcttgg cgtgggaaag
     6841 acacacgaga ttctcgtggc gttgctgaat ccggaagatc gggagtgttt tcctgcccgt
     6901 ggactggtgt actcattcgg aaagccgccc ctggttcagg tgccgtttga agtcagggcg
     6961 cgcgcaggca cactctctga gtacctgccg tacatgccgg agacgtatcc gcggatggag
     7021 accaacctgt tcgatctctt ccagaaccgg attcgtgggt tcggcaaact gaactggggc
     7081 gatgattact ctcccctcta caccaaccag aaacgcggcg gagaggacgt ggtatggaac
     7141 aatctcgaag gcgaccaccc gtaccacgcg ttctgtcagt ttgcgcgtac ggggcagttc
     7201 gcctacttca aggattttca cgacagcatt cttcactgga tggatgtgga ctttgcggag
     7261 cgacatgctg accctcgaca tgagggcgcc ctgattgcgc acacaaggcg acatgtcata
     7321 aatggcggca caacgccgag ccacaactgg gcggaggggt tcaaagagtg gtacttcatg
     7381 acgggtgatc ctcgcgtgga gcgcgtgctg aatcagatgg cagattggtt cttgaggaac
     7441 aaagaccggg aactccgcct gccgccgtac gtgagaactt gggggtgggg ccttatccag
     7501 ctcgccgcga tctgggaagt gacgaatcgg gacgaactcc tggaattgat gcagtggatg
     7561 gtggaacagc ttcatacgta tttcgccgaa cacgaaggcc tcacacaggg tttcggcgaa
     7621 acccccggtg cgtccagcgt gaatgcgttt cacagcgcaa cggtggctat aggcgcatac
     7681 catgtattca agatttcgcg gaatgagacc gcaaggaccc tgtgtctgga agtgactcgg
     7741 agtatcagcg acaggcgcgc gacaagccct gaaggcatcc ccacgtatgt gaacggcccc
     7801 gaacagaact tccctatgca acaggcggcc acgttgacgt taggtgcaat cggttgcgca
     7861 tgggaactta cccgggacaa agacctcgtt cgcagaggca tgagaatgct ggaatattgc
     7921 ctcgaccggg gactgcaggt cgaccacatg aggatcccgg ggcagttcgt ggaatttgaa
     7981 gacggaatct gcctcatgcc ggagctcgtg tggccgaatt gtcaacttct cagttaccag
     8041 ctccgtggga ttctcttgtt catgaaggcg gcacacgaat gcgggtacct caaagaggtt
     8101 gattaccggt tttgaggcaa ctcgttgcgg gacaggggaa tccattgcaa tgcagttctg
     8161 ggcgggaacc ttccgtctgc tgattgagtt acaaagattg ggtcggtaca gagcaacggc
     8221 gttaccaagg acccaaaaac gctgcggtcg agttctgggg cttccagaga ggaagcagga
     8281 aggaagtgaa atccacccga caggaggttc ccgtgagcag aggcgacctg aatatcggca
     8341 gaatggaagg taaacagatc ctcaagcccc gggtggggaa aagcagtctg gcgcgtcgtc
     8401 gcatcggccg cgtaacatcg aaggcgcgcc ggcgatgcgc aaaggcagaa cttctttttg
     8461 agagctggtg gatgcgcgaa gcatgacaca cggcactgtg ccgcatttgc aaaccggcgg
     8521 gcaaacccgc cggttttttt ttgtaaggcc aggacgcgtt gacgtggttc cctgcatccc
     8581 ctatactaca caagctgtaa tgccgtgtgc cggcggcctg agctgagttg atgagcattc
     8641 cgggacggcg tccgtgtgca gacgggcccg tgtgctccat gtcgaaaagg tgacgaaaat
     8701 gttgcagcta cgtggatctt ttgttgctct ggttaccccg tttacgtccg atgaccgcgt
     8761 taaccacgcg ctcttgcggg aacttgtcga atggcacatc gagcagggca ccgatggact
     8821 ggttcccgta ggaaccactg gcgagtcgcc gacgctgacg tatgaggagc acgaagctgt
     8881 tatcgagacg gcagtggagg cagcggcggg gcgcatccct gtggtccccg gcacgggctc
     8941 gaactccacg cgggaagcgg tccacatgac ccagcacgcc aagctggtgg gtgctgacgg
     9001 ggcgctgatt gtactcccgt actacaaccg gccgtcacag gcaggtctca ttgcgcacat
     9061 agagcgactg aacgaagtcg gaatcccact ggtgctttac aatattccaa gccgcaccgg
     9121 catcaatatg gagccggaga caaccgagaa actttcgtac ctcgataatg tagtgggtat
     9181 caaagaagcg agcggaaaca tcaatcagat gagcgaggtg gtaatccgga cacgaggccg
     9241 gtgcgccgtg atgtcaggcg acgacgcgct gacgcttccc ctgctgtccg ttggggggac
     9301 tggcgtcata tcggtggttg cgaatctctt tccgcgcgaa atggcccgcc ttgttgccgc
     9361 ctgggaggaa gggaaacgcg aagaggcact cgaactgcac cagagattgt tcctcctctc
     9421 aaaagcgttg cttgccctgg agacgaaccc gggtcctatc aaagaagcga tgagtatggt
     9481 agggtacccc gtggggcacg tccgacaacc gctcgcccct ctcagcgccg cgaacaggga
     9541 aaggcttcgg aagctgctcg tggaagccgg tctcctctcc tgaacaaaac gcaaggccag
     9601 aaacaaacgc ccggcgaccg tgaggtcgcc gggcactggg aaacaggcgc tcaacgcaac
     9661 cgggggacag tttgcgtttg gcgccgtccc cgaacaacac tcctagtgat gcgcggcgct
     9721 gttctcatgc gcctgctcca acacctgtcg gatgtggccg agtagatcct ccggccggag
     9781 tggcttttcg aggagcaggt ctgctcgaat ccattggcgc tcctgaggag tatccagact
     9841 gtaacggtgc cccaggtgct cgctgactcc cgtcaccatg attatcggtg ttttcgccag
     9901 ttccggtttc ctcttgaagc gctgagcgag ggtgaagccc gaatcagctc tctccatcat
     9961 cacgtcaagc aggatcacat cgggagtgac tttctcgacg gcctccatgg ccgcgctgct
    10021 ggaagctgcg gtgacgacat tgaaccccat cgcatcgaga agagtctcgg tcgcttcgag
    10081 gaagtcctga tcgtcgtcaa tgacgagtac tgtttcagcc attgattttc acgcctttcc
    10141 tttaactgcg ctcgcgcgcc atgtaagtag tgtggagcag gtggtgtgca cgttcagaac
    10201 cgggttcgcc aaggtattcc tcgtacagtt tccggatcgc cgggttctgg tgtgaacggc
    10261 ggtacacctt cgtctggtct atcgtgtaaa tcgcgcgcat tcgagcgcgg accgtctcca
    10321 cgtttgaacc ctgcggcatc ccacccccgt tgatgcaacc gcccgggcat cccatgatct
    10381 cgataaacgc gtaaggactt tcgcccttgc ggatctggtc cataagcact ttcgcgttgg
    10441 ccagtccact cgccacggca acattcacat ccatgtcacc gatcttgatc gtcgcttctt
    10501 tgatgccacg aagaccacgc acgtcgcgga agtcaacgtt agcgagttcc ttgccggtga
    10561 ggtagaacgc cgcagttcgg agtgccgcct ccatgacgcc accggtagac gcgaaaatgt
    10621 ccgcagcccc cgtggaagta cccagcggat tgtcgaaatc acgcggcgga aggttcagga
    10681 agtcgatgcc ggcagtcttt atcatgcggg caatctcgcg aaccgtcaaa actgcgtcca
    10741 cgctggggaa cccgtttgtc tcaagctcag gacgttcagt ttcgaacttc ttcgcggtgc
    10801 acggcatgat ggacacgacg gcgatattgc cggggaaaat tccgtactgc tcggcataat
    10861 agctcttgat cagcgcgccc agcatctcat gtggcgaccg gcaggtgctc aggtgcggga
    10921 tcaggtccgg gtagtaatgc tcacagtact tgatccaccc agggctgcac gacgtgatca
    10981 tcggcaacgg ctcgttgttc ttcactcgac gcaccagttc cgttgcttct tcgacaatcg
    11041 tgagatctgc ggcgaagttg gtgtcaaaca cgcggtcgaa accgatatta gaaagcgcgg
    11101 tgacgagttg gcccgtgtaa atacccccgg gagcggcgcc gaacatctcg ccgataccta
    11161 cccgaacagc aggtgcggtt tgcgccacca cgtgcatgtt cggatcctgc agccattccc
    11221 agactttgcg cacagaggag tgctctcgca gcgcgcccgt agggcactgg atgatgcatt
    11281 gcccgcaatt gacgcaggaa gtgatgtcca gttgcctgtc gtacgccggt gcgacttcgc
    11341 tctgaaagcc gcgcttggtg aacgaaatgg cgccgacatt ctggatttcc tggcagacac
    11401 ggacgcacct gccgcagaga atgcacttct ccggatcgcg ttctatcgcg gggctcgagg
    11461 aatcaaccgg ggcaagcctc cgctccccct ggtaagggat ttcgcggatg ttcaactcgc
    11521 gggtgagcgt ctgcagctcg cagtccccgt tgcgcacgca cgtgaggcat tcctgcgggt
    11581 ggttcgcgag caaaagctgg acgatcgcag tccgcgccgc tctgacacgg gcagtatgcg
    11641 tccggacaac cattccgtct gcgacaggaa atgcgcacga cggaacgagc gtgcgtgccc
    11701 cttccacttc aacgacgcat accctgcagg ctccggtcgg agaaagacca ctgagatagc
    11761 agagagtggg tatctgaaag cctgccagac gagcagcctg cagaatggtc gtacccgctt
    11821 cgactgttat ttcacggcca ttgatagtga ggtcaacggt aggcatagat taaggcctgt
    11881 tcatcctgtg cgccatatgg ttcggttatc cgagcaacgg aacaacattg cagaacgttg
    11941 cgcacaccgt ctgttgtcgt gaattcccgt tgccctcgaa gcgcgataac aaattcgcga
    12001 gtactactct gcgagcactg cgccgaaatt acacacctga cggcacgcat cgcagcttat
    12061 gcatcgttct gatacgataa aatgagcctg tctcttctcg cccagaatcg cgccctgcgg
    12121 gcacttcgcg gcacacaaac cgcatcctgt gcatttggac gtatcaatcc tgtaggtgag
    12181 aagcccgcga catacccccg cgggacactt tctatcaaac aggtgagctt catactcgtc
    12241 gcggaaatac ttcaaggtgc tgagaaccgg gttcggcgcc gtctgcccca aaccgcacag
    12301 cgatgtgtcg gcaacgatgt gcgccaggga ttccagaagc atggcgccat cgaaacgacg
    12361 cagttggtcg tccgtgttcc gttcgggttt caccatggca actcgttcga ggatttcgag
    12421 catcctcttg gtgccttccc gacacggaat gcatttcccg cacgattcac cctggacaaa
    12481 actcatgaag taacgggcga cgtccaccat gcaggtgctc tcgtccatca ccaccattcc
    12541 accggaaccc atgatcgccc cggttccctt gatagactca tagtcaaccg gggtgtcgag
    12601 cagactttcc ggaacgcatc ctccggaagg cccaccgagc tgaacggcct tgaagcgctt
    12661 gcctccagga atgccgccac cgatctcgta aatcacctct cgcaacggca gacccatcgg
    12721 cacttccaca aggccggtgt tgttgatcct tccggtgagg gcgaaaacct ttgtgccttt
    12781 gctgcccggc gttccgatcc ccgcaaacca ggcgccccca cgattgatta tcgtggtgat
    12841 gttcgcaagg gtttccacat tgttgatgtt cgtggggcat ccccacaaac cggaatttgc
    12901 ggggaaaggt ggccggggcc gcggcatgcc tctgcttccc tcgattgagg caatcaacgc
    12961 ggtttcctcg ccacacacaa acgcgcccgc acccattttg agtttcagat ggaagtcgaa
    13021 tcccgtcccg aggatgttgt ctccgagaag ccccgctgtc tcggcatcgg cgatgcaacg
    13081 tttgagtcgt gcgaccgcca gagggtattc cgcacggatg tagatgtacc cctcgtgcgc
    13141 accaatcgcg tacgcgccga tcacgagtcc ttcgatcacg cgataggggt cactctccag
    13201 tacgctccga tccatgaacg ccccggggtc accttcgtcc gcgttgcaga tgatgaattt
    13261 gcggtcgcct tttgccttgt ttgcgaattc ccactttttt ccggtgggga atcctgcgcc
    13321 gccccgcccc cgaagcccgg aggcttggac ctccgcgatc acctcttccg gcttcatctt
    13381 tttgagcact ttggtaagtg cgctgaaacc gttggcggcg atgtaggagt caaggctgtc
    13441 cggatcaaca agcccgcagt tctccagaac gattttcaat tgcggtttga agaacggaat
    13501 gtccgtgatg cgcggcagat ctgagccttc aggcataaca acgccgtcgt tcccaagcca
    13561 gccaagagcg atatctgagg cacatttccc ctcgatcagc acatgctcca cgagacgttt
    13621 cgcaagttgg ggagtgacat tcgcgtaaaa aacgcggggt tttccaggca actggatgtc
    13681 tatcagaact tccttttcgc agtagccgta acacccgacc ggcataacgt ccacggaaag
    13741 attgcgagcg gcgatttctg caaggaatgc gtcatacact gcctgtgcgc cggctgccat
    13801 gccgcaggtg cccattccga tgtgaaccac gggacactca agcagatctc ctgaagcacg
    13861 gtctttacgt tccttgcgca gtgcgctgca tgctgcgtcc tcgtggcact ggggcccgtg
    13921 gagagcgcag tagaccacat cggggcagcg tttctccgcg gagtgccagc acttggagca
    13981 acacggttct agcaatttga acatcattgg cctcatctct taagcaaccg ctttttcggc
    14041 tgcgcctttg gcatcttcac gtttcagcga tttgaccaga cggggaatat ccttcaccgt
    14101 caaacgtccg tggaatttgt cgccaaccat ggcaacagga gcgatggaac acgcaccgat
    14161 acaggccacc gtttcaatgc tgaaaagccc gtcttttgtt gtggacccgg ccttgatgtt
    14221 gagttccatt tccagagtgt ccaacaacgc ggcggagcct ttgacgtggc aggcagtacc
    14281 ccggcagata cgaatgacat gcttgccaag agggtgcagc cggaactggt tgtaaaatgt
    14341 cgccacgccg aaaatgcggg ttgtcgagat gtccagtgca cggcctatcc gatcaagcgc
    14401 agcttcgggc aagaacccat aatgctcctg aacgtcctgg agtatgggaa tcagcgcatg
    14461 gggggccagc ggtttgtaag agtcaaggat ctcgtcaacg gcagcaagat caacctcgcg
    14521 gttcttgtgt ccattgtcga ggcgcgcgat agacgcgaac tccgtcgtgg aaatcatggg
    14581 tctcccatct gtgtggattg atccaaaagg cccagttact aagaataagc ctcgaacccg
    14641 ggtgcgtccg attgcggaca ttcacaccga gtgcgaaaca agtgcaaacc tcgtaccgca
    14701 acatcgctcc ccggacgttt gtcagagaca atgacactca tccgcatggg gccgcagatg
    14761 ggtccaccgg acggtttctg tcgccgaaag caccgtgggg agtctgcggc agacttcctt
    14821 ttcgtcccca cgtgcgttgt tcctgcacgc acatcaaggt tctcccgcgg cacaaaagcc
    14881 ggaagaatgc gtcgaaaaac acagacaaga aaaacggtgc gcatcataga tgagcaccgt
    14941 atctcacaat tgagcaggaa gcacaatgtg atgggcgatc gttacaccat cacgtcgcgt
    15001 tcaccttttc gaaccctgtc gacaagggcg cgcgacgtgc ggcgcagcgc ctccgaatcg
    15061 atccctttga tgtgttcctc gatcgccttg atgccgattt cctttgtttc gggggaggca
    15121 tagtcctcga ggtattccgc aaacgtggac aacgcgttcg ggaggcaata ctgcttgatg
    15181 aggcccggtt tcgcgaggtc catgaaatcc attcccgttc taccggatcg gtaacaggac
    15241 gtgcagaaac tgggtatgta cccgagcgac gcgacttccc gtagaacctc gtccagcggg
    15301 cggtgatccc cgagggagaa ctgagccagt tcttcaggat catccgcttt gtatccgccg
    15361 ggttgcgttc tcgagcctgc tgaaagctga gaaataccga gtgcgaaaac ttctcgccgc
    15421 agacttgggt tctcgcgagt ggaaagaatc agacccgtat acggcacggc caaccgaagg
    15481 atcgtgacaa ccttcttgaa ttcttcgtca gtcaatggat gaggcggatt catggcccac
    15541 ggggcgtttt ctgccgggtt gatgcggggt accgagatcg tgtgaggtcc ggttccgaat
    15601 gtgcggtcga ggtgttccgc atgcatcacc atcgccagaa cctcgaagcg gtagtcgtcc
    15661 agacccagta aagcgcctat tcccacgtcg tcaactccgc cgagttgcgc cctgtccatc
    15721 gcctgaaggt ggtagtcgta ccgcgatttc gggcctcccg gatgcgcacg ctcgaacgtc
    15781 gagcgacggt acgtttcctg gaacaactgg tacgtaccga ttcttgcggc tttcagtcgc
    15841 ttgaaatgct cgacatcaag agccggcacg ttgatgttga cccgccgcac ctcgtctggc
    15901 cccttttttg tcgcgtacac agtcgcgatt gcctgttcaa ggtagtccac gatctgggag
    15961 agaggcccga attcccctac gagcaggagc aggcgcttct ggccaatcct ctcgcacgct
    16021 tccacctcct ccctgatctg gtccatcgtg agagtggctc gcggcacctc cttgttgtct
    16081 cgccggaatg cgcagtaaag gcagttgttc acgcactcat tcgacatgta cagtggcgcg
    16141 aagaacacaa gccttcggcc atagatctcc tctttcaccc agcgtgcggc gtgccccatc
    16201 tccgcgagga gatccgggtc ctcgaccatc aacagacgcg cgacttcctc atgggtgagc
    16261 ccgtgcagtt ccttcgcccg atccagtatc tctcggatct cagatttgtc ggcagacctg
    16321 gatgaagaca acaatccctc tatgtgggct tcgtcgatga tgtccgaagc ctttgcgggt
    16381 tgctccgcag gttgcttggg gcgtggtctt gaaaggggcc ttggctcggg ggtctgcgtc
    16441 acttcaatct ctttcatgtc gaaagtcctt tggagcgctt ttgtttcagg tcttgacgga
    16501 atcaaaccca ggttgagtcg catgcagtac acgcaatctc cgtaccgtta aaaacacccg
    16561 tcaggccgag ttcgttccac cgttcttttg attgaggagt cactgacgaa tctggtggaa
    16621 cggccctcgc agcctcgcca gcgcttcaat tggagatgtg acggttcttc gactcgtcgg
    16681 tacgtggggc ctgccgaatc tgcccggaaa gcagttgttc cctccttgca gcgatccccg
    16741 tgccgtgggc atattactct tgatgcagat tttgcctgaa gacgggacgg tttctgaggc
    16801 gatggctgaa tccccaagcg tgcgtatctc ccctcccaat gatgactcgt tcccgtcccc
    16861 ccagcggaaa atcttcgggt acctgtcctt ccaggttttg ggaacactcg taagtcgcgc
    16921 cgccggtttc gtgcgagagc ttgtcaccgc ggttgttttc ggcgcgggcg cttccgcaga
    16981 tgcgttcatt gcagcactca ccataccgac gctttttcgg gacgttcttg gtgaggacgt
    17041 ggtcgaacgg tccttcatgc cgggtatccg cgagcatctt gcgcgaaaaa accatgcgga
    17101 cgcctggcgc ctggcttccg tctcactcaa ctggatgctg gtagcgctcg ttcttggaat
    17161 gggcgttctg tgggtggccg cgccgtggct ggttgatctc gttgcggcag gtatccacca
    17221 gcggtcggtc agcgagggga cggtagccga cgtcatcgca atgacacgca tattgatccc
    17281 ttttctggtg ttcattgcgc ttggtgctta tgtcggtggc gtgctctact atgggttcga
    17341 ttttcacctg gtcttcagtc tcgcccccac gatgctgagc gtaggtgtga tcgcgattgt
    17401 attgttcagc tcgcaggata tcgggattta cgctctcgcg tggggattca cagtgggagc
    17461 ggttctgcag tttgccgtgc aggtgccgtt tcttggctca cagaaaatcc gatccacgaa
    17521 tccagagtac actctgagct tgcgactgaa ccctgcggag cggtcgcgaa tatctcggga
    17581 aacggcccac gtcgcacttc agtcgctgct cacgaaaacc acagaaatag tcgatcgcag
    17641 agtcgcgtca tttcttgtag agggaagtat ctcaagcctt tggttcgccg cacggcttgt
    17701 tcagcttcct tttgcgataa tcgggatagc aataggccgc gcagttgctc cgtatctgtc
    17761 tgagaaagtg ggaactggcg acctgaggga attcaagaat gcggttctta tcggataccg
    17821 ctacaatctc gttctggtgc tccccgttat cgggcttttc accgtgctgg cacacccttt
    17881 tgtccggctg atttatgagc ggggcaattt cggcgcacac gacacgtcga tgacggcaag
    17941 tgctttctgg gcatatgcct tggggttgct cgggctctcg ctttacacgc ttggaacgcg
    18001 catctgctct gcgctggaac acaatcgcgt ggcaacgtac accgcggtgc tcggcgcagg
    18061 tctgaatgta tggctgaatt acgttctttc tgccaccttc ctccgtcacg ccgggcttgc
    18121 tcttgccacc tccattgcct tcagcgtcaa tgctgcctta cttctcatgt gggtacaccg
    18181 atatctggct cagtgtgggc aaggcttccg cactcgggaa ctgctcgtgc ctttcctgcg
    18241 agtcctggcc aacaccgcag gggcagtgct ggcagcctgg gtggttttgg cgttttttgt
    18301 acctgaatcc tggttcgtaa cgcggagttt taccgcttcc gtgggcgctt tttgtgtgcc
    18361 ctttgccggg ggcttccttg tgtatttcgt tcttgcggtg attaatccgg ttgcggagat
    18421 agtacccgtg aaacgctgga tgaaacgctc cgtgattcgc tttggaaacc gttgaaaggg
    18481 aacgcaggac tccgtatcaa ggggcacttt cgccacgcag gttctgaatg ggacatcggt
    18541 tcatactgac gggaggcggc acgggcggcc atgttacacc ggccatagcc cttggtgaag
    18601 aactgcgtgc ccgcgaacct gacgcgcagt ttctttacgt tgggacgcgg accggcaagg
    18661 aagcggagat cgtgccccgg tacgggttcc cgatcgcgta tgtacaaagc caccagtgga
    18721 gctttcgtcg gaaatggcac gttttgcgct ttatctggcg catcgcgtgt ggaaccatca
    18781 aagcgtgcca tctcatcatc aaataccgac cggacgcggt gatcggcacg ggagggtatg
    18841 ttgccgcgcc tgtggttttt gcgaacgttc ttctccgccg aattcgactc tcccgtgccc
    18901 tgacaatcat tcacgaacag aataccgtcc caggaaaact gaacgcccta gtgggcaaag
    18961 ttgcggacct tgtgcttctg agcttcccca ctgcctccgc tcgtttcccg cgggcggtgt
    19021 gcgttggtta cccggtccgg ccggaaatcg cgccggcgga tcggacggaa gcaaaacgcc
    19081 gactcggaat cccgcaggag acgacagtcg tactcgcttt tggcggctcg atgggagcga
    19141 gaacgatcaa ccgcgcagtc gtggacgcgt tgcccttcct ccgggaccgc aaggacctcc
    19201 acatcgtcca cggaaccggg cgaaagctct cgtcgtatga cccagttgcc gactgtgagt
    19261 cgcggatgca acggaacgga cttacgtacg agtccctttc gggatggtac gaggcacacg
    19321 ggttcattga ccggatcggc gaatactacg ccgccgcgga tgtcgttgtc tgccgttctg
    19381 gtgccgggac gctggcagaa ctttgcgcct gcgggcgcgc ggctcttctc attccgaagg
    19441 cgaatctgcc cggagaccac caggtattga atgctctgga gatggcacgg cgtggagcag
    19501 ccgatattct tttcgaagat gcggtgacta cgaacggtgc gccggaggaa tacgtggccg
    19561 gcgaggaact ggcccgcaag atcgtggagt tgctggaaga caagccgaga ctgggcaaaa
    19621 tggagtcgaa tgctctggcg agcgcacggc ctgaagcaac caaggtctca gtcggcgtta
    19681 ttctttcgtt gcttgaaggc aggtctccgg acggtttgtt gttggaattc gccgcgcggg
    19741 aaccggtggt gggagacgcg cacctcgaca cgaggtttgg acgcgtcgct gcgctctcag
    19801 gagaggcgct ccgcgcgtat gcggagacgg cagtatccag catctctcgc agcctgaact
    19861 gcgcgtccgg tacgccatcg gaccaggaag acgctcttgt ccgtgccctg gatgcggacg
    19921 aaatggtcgc gtatctcaga taccgggccg ccacacacct gacatcgaag aactggcgaa
    19981 tgcggaatgc cggtgtaaaa atggtgggga tcctccggca gcgtgacaaa cttccggttc
    20041 tggccggatt cgtgaaggat cgagcgcccg cctcgcgcat gcgccgactc ttcggcggcg
    20101 atttcgaaac gaacgggttc attcgtcgca actcaatggc aagcctgatc aggctcggag
    20161 tggcgtctcc tcttgtgcgc gaagtcatcc tgtggggact ggaggatccg tatttcgagg
    20221 ttcgttcgca atccgcgcgc gcggtacagg cgttccgtgc gagtctcgaa aatgatcagg
    20281 aaattgtcga gaggttgctg aattgcgcga aggacaaata ctttgaagtt gcagttgaag
    20341 cagtgaaggc tatgggctcc gttgcttctg cagaaacggc cctggaggtg ttccgctctc
    20401 tgtcccagca cgagaactgg cgagtgcggc aggcggtggt ggaagcgtgc tgcgccctct
    20461 tggatagagg gcaggacgta ccgaaggcat ttttgagcga aatactggcg gggatgatgg
    20521 tgacaagtac cgggttctgc ccatcgtttt cgctgaaact cgcggtggcg aagctcggag
    20581 aacgaatccg cgaacctgac agcacagcgg gcgaccgagg gaaatagtaa tgctttatca
    20641 ccttgcgtcg gaggcattcc agtggtacag gcaggcgccg gtcagttttc cggtacttgg
    20701 atctgacgta ctattgctga cccgcttcct catgtacctc tccgttcgtg cgatcctcgc
    20761 gatactgacg tcgtttgtcg tcaccatgct cgttggacga aggctgatcc gatttctgca
    20821 tgcgcaccat gtgcgtgaca cggtgaaaga tttcgggacc atttcagcga cggacaagcg
    20881 cggaacgccg acgatgggcg gcctcatcat catcacttcc ctcgttgtcc cggtattgct
    20941 gtggtgcgac ttgaccaacc ggtttgtgca gattgcactc gccgcaaccg catggttcgg
    21001 agcgctcggg gtcgtcgatg actacctgaa agtacgaccg tatcgtggac gcattcgcgg
    21061 actcggcgag gctcagaaac tcgcgcttca gggattgttt ggtctggggt tgggcattgt
    21121 atatctgaca ccggcgattt ctcccgtacc tcaggaggta gcaggcagct tctacgtccc
    21181 gttttacaaa tatgcggtcc tgcacgacat cggctggtgg tatctgccgt ttatcgtgtt
    21241 cgtaaccctg ctcatttcga acagcgtaaa tctcaccgac ggcatggacg gcctcgcgat
    21301 catgcccgcc aacttcgcgg ttctggttta cggggtcatt gcatacgcaa tgggtaactt
    21361 agtgtatgcg gagtacttga ggtacgatta cctgcacggt tccggggagt tgctggtatt
    21421 ctgcgcagcg ctgttcggag caggtctcgg gtttctctgg tacaacgcat atcccgccga
    21481 gatcatcatg ggagataccg gctcaatggc actcgggggt attcttgcgt ctctggccat
    21541 catgttgaag caagaggttc tttttctgat cgtgggtggt gtgttcgttg cagagactct
    21601 gtcttccttc attcagcaga agattgggga aaacttcctg ggaaggcgca tcttttatcg
    21661 tgcgccgctg caccaccaga tggaataccg gggcctggcc gagacgaaag tggtgatccg
    21721 cctctggatc attgcaggtc tgcttgcgat ggtcgggctt gcctccatga agctgaggtg
    21781 atttcccctt tcttcccgcg tgaaatctcc cctcctcgtg cgtccggtca tgtcagcgct
    21841 cgtttgtttg cgagtgcgca tggaccaaat gagatccacg agtacaagat tcagcagctc
    21901 gttttgacca tccgtttccg ctggggagcc acgctccgcc ccacccctgt ctagcccctc
    21961 ggcaagtccc gcgttatttt ccttctccat acaggctgtc gccgtctggt cagcattccc
    22021 gggccgattt ccttcattgg ttgaggaagc aacatgattg atatccgcag cgacacggtc
    22081 acaaaaccca ctgctgccat gcgcgcgttc atggcgaacg ccgaggtggg agatgatgtg
    22141 taccgcgaag acccgatggt ccgcgcattg gaggaggaaa cggccgccct gttgggcaag
    22201 gaggccgcac tctacgtccc ctccggcacc atgagcaacc agatcggcgt atgggtgaac
    22261 acccatgaag gcgacgaagt gatctgcgag cgggactgcc acatcttcaa ttacgaagcg
    22321 ggtgcgccgg cgctgctctc tcgggttctc atgaaaacgt tgcagggagc acgcggggtg
    22381 atttcggccg cgcaggtgga ggaggcagtg aatcctgcca atatccacgc tgcggctact
    22441 accctcgtgt gtctggaaaa cacgcacaac aaagccggcg gcacgatata tccgcgggag
    22501 gaaatggagc gcatcagcga cttgtgcaaa cgaggggaca tgcggctcca ccttgacggc
    22561 gcgaggttgt ggaacgcctc tgcggccact ggaataccgg aacgcgatta tgcagaactg
    22621 gcggacaccg tatccgtgtg cttctcgaag ggtctgggcg cacccgttgg ttcggccctg
    22681 tgtggttcac aggaggtgat tgaacgcgca cgtcgaaaac gcaagattct tggtggcggt
    22741 atgcgacagg caggaatcat cgccgccggt gcgctctatg cgctgaggaa ccatcgtgtg
    22801 cgcctggtcg aagaccacgc gaatgcgcgc tgtcttgcgg aagcaattaa cgagttaccc
    22861 ggtatcaagg tcgatcttga gggcgtccag acgaacatcg tgatgattga gatcacaggg
    22921 aacgcgacgg cgcagcaggt ttcggcaatg atgaaggagg aaggcgtcct catcaatgcg
    22981 atgcgaccga aagtcctgcg ggccgtcacg catctcgacg tcagtgccag ggacatcgaa
    23041 aaggcgatcg aggcctttgc gcggatcagt cgcagaattc ggagttgaac ttggaggagt
    23101 tttccatgaa gatcgcgttt catcagatta cttcggggtc caagagagac ctgaaagaga
    23161 cgttcaaagc ctacgcggaa gccggatggc gccacttcga ggtgaacctc tgggaaacgg
    23221 aggcgtttgt cactcaacat ggtgcgaagt cgctggccgc tctcgcgaag gatttcgggc
    23281 tccattgtgt cggtgcgaca ggtctcagca tccccgcgtt tcagggtgga gaagctcacg
    23341 aggctgcaac aaaacagatg gagattcacg cgcaggtgat gaatgccctc gagtgcacca
    23401 cgattgtggt gggcggcgac gtgccgaagg atttccctcc tcgcgcgcag aattcgacgg
    23461 aaaaagcgct ttcagcacgc gatgccacgt acaggaatgc actgaaggaa tttgcgatgg
    23521 cggtcgaacg ggtggcggaa accgcggcga aatacgacgt cacactggcg ctggaggtca
    23581 actggtgcgg tctggcgaga agcttccgga cgatggcgga gctggtgatg atggttgatc
    23641 gggataacgt gggtgccacg tgggaccccg cgcactttta cagcacggcg tcgcgccttt
    23701 ccgatctgga ccgcctcaga ggcaagatcg tgcacgccca tctcaacgat atccgagact
    23761 gcttcatgga ggccatggac atcaacggcg accgcgtgct gcccggcgac ggtgtgttgc
    23821 cgctcgtgga atggacggac aagattcatt ccctcgggta tcgtggctgg cattgcgtgg
    23881 aattgttcag cgacgatctc tgggcggagc cgctgcacga gattgcgacg cgggcaaaaa
    23941 aggcttgcga acgcgtgtgg ccagacgcgg agttctgatc tccagtcgaa aaggaacgga
    24001 tgagttcccg caggtaggcc tattgcagcg gcgagatgga cggaaaagga aaaggcttct
    24061 catggaaatg aatgaacagg aacagaagat tgccgcagcc cgggagatga gggagcgcct
    24121 gctgcgcgac cggtatcggc ccggctacca cttcgccatc ccggaagata tcgggatgcc
    24181 gggcgaccct aatggcgcct tctgggccaa cggccgatat catctcatgt atctctacaa
    24241 ccggcgcggg gcaaaagcgt ggggcaatgc ggcgttttgc tggggccaca tttccagcgc
    24301 agacctcgtt cactggcgac accaccccga cgccctcctt cccggcgacg gcgatggtgg
    24361 ctgcttcagc ggcggcgctt ttgtggacga ggatgggact gcctatctga cgttctggcg
    24421 attgccactt gccgacggga aaacggaggg cacggggatc ggaatcgcga gaagtagaga
    24481 tcgcaattac gacacctggg aaaagatgcc cgtccccacg ctcgatggga ctgagttcgg
    24541 cattcgggag acccgcgata gcgaagggaa ggcgtacccc ctgtgcaacg ccgaccccag
    24601 caatatctgg aaaaagaatg gcacgtatta cctccagacg ggcaatctcc tcgtcctgag
    24661 gaaaatggaa aaatctgacg atgcctcggc gaaatggaag ggcgactggg tcgatctgtt
    24721 ccggtcaagg gatcttgttg attgggagta cgtccacagg ttctacgcga gaaacccgac
    24781 gaacaggtgg acggacgaaa gcgaagacga catgtgcccc agctttctcc cgcttccgag
    24841 ttcgccttct ggtggctttc cgagcggcaa atacctccag ctcttcattt cccacaacaa
    24901 agggtgccag tactacatcg gaacgtatga cgaggggagc gacctcttcc tgccggagac
    24961 acacggccga atgacctggg ttgacaacac gtttttcgct ccggaagcgc tgatcgacga
    25021 caaaggccgc cagataatgt gggcatggct tcttgataac gcgccttccg aaccggaaca
    25081 acagattctg acggggtggt ctggagtcta ttgccttcca agaaccctct ggctcgggga
    25141 agacggaatg ctgcgaatgg cgccggtacc cgaactgtcg gtgctgaggt acaacccccg
    25201 agttttcgac gacgtgaacc tccacccggg ttctgagaaa accttggaag tggccaacgg
    25261 cgagtcagcg gagatcaatt tgtccatcga atcgttgacc gcgacgcagg cgggtctgaa
    25321 agtccggacg agccctcatg acgaagaaac gacgttgctg tactgcgatt ttgaacacaa
    25381 caagctggtg tttgattcta cacggagcgg gaacgcggga cggaaagtca aagaggaagc
    25441 gccctttgag tgccgcaagg gcgagcgcat cgatctgcgt gttttcatag acagggcggt
    25501 ggtggaagtt ttcgcggggg accgccaggc gatcgctcgc agggtctacc cggttcgcga
    25561 tgacagtacc ggcgtgcgcc tgttctgtgt gggaggcgaa gttgcattta ccatgatcag
    25621 aacatgggaa atgatgccgt cgaacgcgtg gtgatcgggg gaaaagagcg cagtgcgcaa
    25681 gtcgcccggg tggcggggag aaagacaatg gaacttcacg cacgtacgaa ttgcatcgta
    25741 gagatacctt tccgctccgc cgcggtgtac aggaatcctt acgcggatgt tactctggac
    25801 gtggcgttct cgaactcccg gggcgcgaaa atcatcgttc ctgccttctg ggccggcggc
    25861 ggaacctgga aagtccggtt ttccgctcct tctgcgggaa cctggaagtt ccagtcaatc
    25921 tgcgacaacc ggtcagacat cggtctgcac gggagaaccg gcgcgatttc tgtccaacgg
    25981 tatcgaggca gtaatcccgt attgaaacat gggcgagtgc gtgtttcccc gaacgggcgc
    26041 tatctggaac accaggacgg cacaccgttt ttctggcttg gtgacacgtg gtggatggga
    26101 ctttgtacgc gggttgactg gccacagggg ttcaaggaac tgaccgcgga cagggttgcg
    26161 aaagggttca gcgtagtcca gattatcgcg ggaccctacc ccgacatgga tgcgttcgat
    26221 ccacgggctc ggaatgaagc cggttttgcg tttgagaggg ggttcacctc tgtcaatccg
    26281 tcatattacg accacgcgga tctcaaaatc ggacatctcg tggaatccgg gctcgttccc
    26341 tgtatcgtcg gaatgtgggg gtattacctc cccgaaatcg gggtcgaaca aatcaaacgt
    26401 ttctggcggt acctcatcgc gcggtacggc gcgtatcccg ttgtatggtg cgccgccggc
    26461 gaggggacca tgccgtggta tctgtcgaaa acgcccgcag aggacgctca gaagcagaaa
    26521 tcaggctgga cggacgtgtg ccgttatatt cgctcgaccg atgggtttgg aaacgtgctg
    26581 accattcacc cgacccggta cgggcatgag caggtggaag acagagcggt acttgacctg
    26641 aacatgttgc agaccgggca tagcggttac gaaagcgtag gtccgacgat cgaaagtgtg
    26701 cgcaccgcgt acgcgctcaa tccacccatg cccgttctgg atagcgaagt gaattacgag
    26761 gggattctcg gcaagagctg gcaggatgtc cagcggctct gtttctggca ttcgattctc
    26821 aacggtgcgt gcggccacac gtacggcgca aacggcatct ggcaatgcag tacgaaagcg
    26881 gagccgtacg gtgcgtcacc acacggacgt tcctggggca acacaccctg gcaggaggcg
    26941 atgaagcttc ctgggtccac ccagctcggg ctggcgcgaa agttcctcct gcggatcgat
    27001 tggcaccaga tggaacccca cccggagtgg acgcaagatg gcgccagtgc agccggcatt
    27061 cccaagcggt tgcgcgtgat ctattcaccg atggtctggg atcctcccgc catcaaagaa
    27121 ttggaacccg atgtgaaata ccatgcacgc ttcttcgacc cggttacagg tgcggagtat
    27181 cctatcggtc cggtggatcc cgctcccgac agaacatgga cgccccctgt cttccccgaa
    27241 atgcacgact gggtaatcgt tctgaaagcg gtcaccggat gacctgggcg caagtatttt
    27301 cccggctcca ggcaccgcga aatcgagccg cgcatgacct cgcggatggt cgggcgaatg
    27361 ccttctcctg gtccagaaaa cacttttccg ggcgagtgtc caaccgggtt gctcctgccg
    27421 acatgaacct gaaaagtcaa ggacctcaaa ccggctgccg agaatccgcg tttcccacaa
    27481 tcgtcgggct ggtggcattg gtggtgacaa gcgggcaaac ggttgccgca acgtcgataa
    27541 gcggatacgt ccg
//
LOCUS       DAOSOO010000017        26752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1808_length_26752_cov_5.352586, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000017 DAOSOO010000000
VERSION     DAOSOO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 26752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26752
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_26752_cov_5.352586"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLT86_02420"
     CDS             complement(<1..584)
                     /locus_tag="PLT86_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT35501.1"
                     /translation="MRAGRDFPTPESNRHTVAELIGKYIANALPRKRLSTSRNQASQL
                     EWWRERLGHYLLQELRPALLADAREGLSREPTPSGKPRSPSTVNRYMAAMSHCLTYAV
                     RDLNWLDDNPMRKVSRNKEPQGVVRYLSDQERSRLLSVCKQSSSSFLYPIVLLALSTG
                     MRRSEIMNLTWDRIDFLRDAITLRSEPPSSWWRR"
     gene            complement(1141..1214)
                     /locus_tag="PLT86_02425"
     tRNA            complement(1141..1214)
                     /locus_tag="PLT86_02425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Met,seq:cat)
     gene            complement(1264..2706)
                     /gene="ftsZ"
                     /locus_tag="PLT86_02430"
     CDS             complement(1264..2706)
                     /gene="ftsZ"
                     /locus_tag="PLT86_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT35502.1"
                     /translation="MSNDNFELIHEQEPVARIKVIGVGGGGGNAIRRMVDSGLSRVEF
                     IAINTDQQVLHRSGATTQICIGNNVTKGLGAGGRPEIGRQAIDEDRDRVADALGGSDM
                     VFITAGMGGGTGTGASPVVAQIAREIGALTVGVVTTPFAFEQPKRLRRAAEGLEALRE
                     AVDTLIVISNERLLQVLPPRTPINDAFREADKVLLQAVQGITDIILVPGLINLDFADV
                     RTVMSHRGDATMGSGVATGENAASRAAEIAIASPLLEDVSIRGAKGLLVNITSNSSLP
                     QEDVNAAMSVVNREAGADADVFFGVVLDDSVPEGEIRVTVIATGFESRCPSTFTREIM
                     TPAQKPFAVPTSPTVSVNTLEPEREPEPEPVAEAVPEPVELPVEEKVERNTVETVVNI
                     PMTENELQIPAWQRKQQEPLQAPAEEPSSLGTAAHVLVPKEPKVAPVSSVPRESLDRE
                     LPRQYGIYAGSTDSADLRMPTFLRKARGRT"
     gene            complement(2810..4126)
                     /gene="ftsA"
                     /locus_tag="PLT86_02435"
     CDS             complement(2810..4126)
                     /gene="ftsA"
                     /locus_tag="PLT86_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT35503.1"
                     /translation="MPLGRIVAGLDVGTTKVTTIIAEVDESEAVSVIGVGQTESRGVE
                     RGMIVNLDETVDSIQKSVERAQHMAGCRISSVYVGIGGEHIRGINSHAAVTVGKITNE
                     ISEEDVKRVIDHAQNVSMPADMTVIHVIPQEYRVDDQKHIIQPIGLAGSRLEGEVHIV
                     RGAATAEQNLRRAVQRAGLQVAGIVIQPLASALAVLSPDEQEWGVAMLDIGGGTTDIA
                     VYHRRCIRHTAVLSIGSAYVTSDIAIGCSILMSEAEQLKKQYGRASRQLAEQEHNLPE
                     DPREQPDIRFLRENTRAVLDAKIPEPKFSVELARIIEMRMVELLGAAWFEIRKSGHQE
                     YLASGVVVTGGGAMVKGVAELAQRVFGVPVRIGYPLGVGGIRESVATPANATGVGLVL
                     FGASQLLKTPQTMSMTDAERELERLNQEMQRKPLEGMRRWLKEFFA"
     gene            complement(4153..4971)
                     /locus_tag="PLT86_02440"
     CDS             complement(4153..4971)
                     /locus_tag="PLT86_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT35504.1"
                     /translation="MAEETPPTTSESGTGLRQRVWAGLVTHKTRVFSRWPWIARLVAF
                     VAYVATGAALWGTGYGIIRLPWLAIDDVVVVGTDQVDVRAIYDAAGIRRGSPLLALDV
                     NAVTAGVKSVPWVRDVRVSRHLPGTVRVEVVERAPVAVFQLDRCYLMDEEGFVTPLNG
                     RTVPDLPIVTGLKAVPFPQRESLEPAGRALAVVAEFPVLRNTVSELSLADSTRMVLIL
                     SPRGTPVSLPRLTGRDRLVMLASVIAYEPDLLMTASHLDARFLGHLAVTSSPSS"
     gene            complement(4973..6376)
                     /gene="murC"
                     /locus_tag="PLT86_02445"
     CDS             complement(4973..6376)
                     /gene="murC"
                     /locus_tag="PLT86_02445"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT35505.1"
                     /translation="MNGESVVPMFHKIRRIHMVGIGGSGMSGIAEVLHSLGFAVTGSD
                     RLLSPVTARLESIGIPVAEGHDAANVRGAQVVVYSSAVKADNPELLAAQAGKIPVIER
                     SEMLGELTRMRFTIGIAGTHGKTTTTSMVGQLLTHAGMAPTIIVGGITKSLGSGGILG
                     KGGYFVVEADEYARTFLRMFPTIGVITTLEADHLDCYRDFGDIESAFKAYLERLPFFG
                     VAILGIDDPNVRALVPSIRRRVVTYGFAGDADVRAIDVRSREKGSRFSVVANGENLGE
                     VALAVPGRHNVQNALAAIVVARNLGVSVQQIREGLAGFSGVDRRFQVRGKVHGATIID
                     DFAHHPTAVRVTLDTARNMAGEGARLLVAFQPHLFSRTRDMYEEFGAALARAELVLVL
                     DIYPSRETPIEGVTSRLVAEACSRAGARDVREVHSHEEAAAILRDSARSGDIVLTIGA
                     GDIWKVGTALAETEENS"
     gene            complement(6373..7602)
                     /locus_tag="PLT86_02450"
     CDS             complement(6373..7602)
                     /locus_tag="PLT86_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOT35506.1"
                     /translation="MEAPLIDANSLNWVHERLGDTGASRKQVVREAAGSGKAEGAEQA
                     LASSSQPLDVILLAATIMIVGFGATMVYSSSSVIAAEKFGGPAHFMVRQVTRAALGLL
                     CLIAALFVPVDRVRKYFPYFAFASFSLLLLPALFSVAYKGARSWVNLLGLTLQPSEIV
                     KFALILYLADRLTKHQERLESLKDGVLPLIGLICIPLGIIVLEPDVGTAVAMASVFGV
                     MMFVANIRMRHILPLMGAVALAVYVLIIIEPYRLKRLETGYQIQQGFVAMGSGGLLGR
                     GLGRSIQKYFFLPEPYTDSILPIIGEEFGFIGTVAVVLLFCLFGWRGLRIAYQQPTLF
                     RFLLATGLTANILMYALLNMGVMTALMPTTGLPLPFISYGGSSLVLNLAGTGLLLNLS
                     RQRRLGEGEEEVKEDTE"
     gene            complement(7620..8999)
                     /gene="murF"
                     /locus_tag="PLT86_02455"
     CDS             complement(7620..8999)
                     /gene="murF"
                     /locus_tag="PLT86_02455"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT35507.1"
                     /translation="MDAYSPEQIAEITGGTVLNGSQGPVTGVSIDSRRVNKGDLFVAV
                     TTQTNDGHRFVGDAFAKGARAALVDRRKVEAHLREWDIYTLITVDDPVAALQKLARAH
                     RRKFAIPVVAVTGSNGKTTTKEFIASALAGLGPVLRTEGTLNNHLGVPLTLLEITEEH
                     RAAVVEMGMNHAGEIHLLGTIAEATVGVITNTGQAHLESFPSFDALVDAKWELVGTLT
                     GDRMLVLNRDDVGLVARARRWDGTIRWFGIESDCAFKASALQQGQDGCWSFVVNGSEV
                     QLRIPGRHMVYNALAAIAAADALGIPAGEAAVGISRTEPADRRMRSLWIRGVLVLDDA
                     YNANPTSVRAALQTLMTLEPGKSGRRIAVLGGMRELGEQSLALHREVGSYVAGLGVNL
                     ITVGELGNIIAQGARSAGASETPGFETQQACAEWLRNNLREGDVVLVKGSRSEHMELV
                     IDHLQKALS"
     gene            complement(9018..10493)
                     /locus_tag="PLT86_02460"
     CDS             complement(9018..10493)
                     /locus_tag="PLT86_02460"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT35508.1"
                     /translation="MADTVPGSLFFSGSAGDRPLTCIVTDSRTAREGALFCALPGTKV
                     DGWRFAAHACERGASAVLTPVRIEGLAVPQIVVPDVRRALAVVASAWYGNADRNTALV
                     GVTGTNGKTTTVRLVGSVGAAGGFVPSTIGTLGATLRGEDFPFDTAWNTTPEAHELRR
                     LLKALNDAGSTLTAMEVSSHGLALDRVWGLSFRVAVFTNLTTDHLDFHGSLDEYFRAK
                     ARLFEDLAPSSTAVINIDDRHGQKLVGRTAAKVLTYGFSPSADIHPLEVADLPWIKGR
                     VKLPQGTITLEMPLAGRFNVANAMAAIGTGLALGLAPEEIARGLKDATPAPGRFEPVQ
                     AGQDFAVIVDYAHTPDALENVLMAARSMTGNMGRVICVVGCGGDRDPFKRPVMGRIAS
                     EIADVCVFTSDNPRSEDPEAILDAISEGVPKGRTFERIESRKAAIRHAISVARRGDIV
                     VIAGKGHEPYQEIQGVRYPFDDRLEARAALEELAGKTIKKP"
     gene            complement(10520..12988)
                     /locus_tag="PLT86_02465"
     CDS             complement(10520..12988)
                     /locus_tag="PLT86_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT35509.1"
                     /translation="MPTTQHEPGLERDVALEASAVRRRVPPPDTDHKRPGLPFPGELR
                     TAPVEANPPRPDFVTAPGQTPPLEPKYRPYDGPAQRAHTIRLVIVGILWLLGLGAITS
                     RLVKIQILDHEKYLAKAAGQQERQITLAARRGKITDRTGRDLAVSLAAASVSVNPRKL
                     TEMKLLSERVSEVSGEPAQRIYQRISKPGRAFAWVARNIETDQAQRIFALAPHAMTIH
                     PVTKRKHPLGGLAGQVLGHVSIDNVGGEGLEALYNQQLRGKDGWMISLVDGLGEQVPN
                     TAQPLMPPVHGNDLTLTIDADYQAIVEQELSKMVDSLHAAGGLAVLYEASTGAIRAMA
                     NVPTYNPDESSQTRNGALRRNRAITDPYEPGSMFKAITVSAAMAEGKVTPDDMIDCQG
                     GRISVAGGMIRDSHPNGVLDVRGVIAHSSNVGTIKISQRISSETFYGYMRAFGLGSVT
                     NVDLPGETRGLLARPSRWSARTHATIAMGQEVGVTALQMAVAYGAIVNGGVLMAPRVV
                     ERITTPDGKIIHAFEPRKVRQVIPAAVADSMVNMLCGVVEKGTGKKAAIPGVRVGGKT
                     GTAQMAAAGRRGYERGAYIASFIGFLPDMDPRLVCVVSVTKPQGSYYGATVAGPVFKN
                     IMTRIINRDAAVLQRPSDAVYPRMPEVVGMSEIGATARLASLGIPVRRSGRGSVVVSQ
                     WPPPDCRVSPNRPALMTLAEREGLANVLPDVTGTPVRQAVATLSAAGFVPRWEGSGIV
                     IAQEPAPGTLPEPGCTVVLRCQRALGTAGAMLEPDGRETPSEERQEPEASVDDADVVM
                     IDASDVASKPTSPGAPIIRRGRSQ"
     gene            complement(13044..13409)
                     /locus_tag="PLT86_02470"
     CDS             complement(13044..13409)
                     /locus_tag="PLT86_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOT35510.1"
                     /translation="MRAHNLFWGAAIVVLAVATMIAFVWKGWHSRQLALRLEQLRAER
                     RELLDEQDKLRARATALSHAARIKDIVSERLGLVEPTTPPVDVPAIGSLGSPDSLAEC
                     RATGTEILRAMRSRSSSGQ"
     gene            complement(13406..14335)
                     /gene="rsmH"
                     /locus_tag="PLT86_02475"
     CDS             complement(13406..14335)
                     /gene="rsmH"
                     /locus_tag="PLT86_02475"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT35511.1"
                     /translation="MPEHYRWHVPVLADQAVEALVWRNDGTYVDATVGDGGHAEKICE
                     RLAGGGRLLAIDRDPEALERAADRLNQYKDRVVFARASFARLDELVENAKLGHISGML
                     FDLGVSSRQLDQPERGFSYRSEGPIDMRMGPDTETAAATIVNEWPEPELARLFRVFGE
                     EPHAKSIARSIFQRRSRRPLATTSDLVEAIGRRGTHPEKTLSRVFQALRIAVNDELAS
                     LNRVLGLVPDLLERGGRLVVISYHSLEDRSVKAWMRMETQGCICPPTVPECRCGHVPR
                     MRAITRRAVMPTAEEIAGNSRSRSARMRVAERL"
     gene            complement(14341..14844)
                     /locus_tag="PLT86_02480"
     CDS             complement(14341..14844)
                     /locus_tag="PLT86_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT35512.1"
                     /translation="MATGPLGVVRRQASFVGNYRHVVDAQGRIQVPRAIRKAMLPESQ
                     GTLYVTRGLDGCLFAYPLDTWQNLAEQWELALNDARSFEKRNEIRVMTSHACEARLDG
                     QGRISIPAYLLKAVDIRSEALFVGVTYRIEIWNPDRYEAAIAAAENSFEQNMRNLVSF
                     RQNRQSG"
     gene            complement(15159..15428)
                     /locus_tag="PLT86_02485"
     CDS             complement(15159..15428)
                     /locus_tag="PLT86_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOT35513.1"
                     /translation="MERHFHARLRLVAAFLLLSFCVVFASGQAWAGEALVGDVTLWRN
                     AHFVVAAYGAVWLGTVLYILHLLRRIAALERDIARLQADQKRTSS"
     gene            complement(15448..16146)
                     /locus_tag="PLT86_02490"
     CDS             complement(15448..16146)
                     /locus_tag="PLT86_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HOT35514.1"
                     /translation="MTTKTGLSRPQTALTILAAVGFPISLYLIFVVTPLDAELGFTQK
                     IFYFHVPCAWITYLAFFIGSGSGVAYLLRRSRRWDHLAHASIEVGTVFTTLVLVTGPL
                     WARPAWGAYWVWDARLTTTFVLWLIEIAYLLLREFVEEPEKAARFCAVLGLLGALDVP
                     VIHFSVVLWRGLHPEAVVLSQRGFASGLPADFLVALVVSLATFTVLFGVMLSLRYRVL
                     RATEQVDYLRCGAG"
     gene            complement(16143..20063)
                     /locus_tag="PLT86_02495"
     CDS             complement(16143..20063)
                     /locus_tag="PLT86_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT35515.1"
                     /translation="MNLFKKFFNKSKREIKRLRPVVAEINAKYELLHDLPDEAIKGKT
                     DEFRKRLADGETLDDLMVEAFAVVKETMRRFTERKMEWDVCGNMTPWFMVPFDVQLVG
                     AIVLHQGKIAEMATGEGKTLVAVLPLYLNALPGKGAHLVTVNDYLARRDASWNGPIYH
                     WLGLSVSVLQDPNANNGIESYKVDWNETQGYHLVPISRREAYACDVTYGRQDQFGFDY
                     LYDNMAWDIADIRHRGFNFAIVDEADQLLIDEARTPLIISGQVPESKSERYVELKPRV
                     EKLVKEQSRIVAELVDDGERLWEAGETYAGAKKLLQAQRGAPKQRKLMRLCESGEVKK
                     AIVRVELDYMRDKRTPELDEPLLYSIDEKNHSAEITDEGRSRLLLTEDKRRFISLMDT
                     VRRIERDENVDDAVKQERLEKEFRAYINETKGRAITSEAGDVVRAIELDEVLTDEQKR
                     VLLKEQFALAVDYASRGFIRFSLTYEAEGPEDDSQADRDAGRAKIVESASNWVKQAIK
                     RLSYPGREIEERISANEYLTSQDKGDLTKALSDAFSTAEREGLSRPSFDDVTAFVTHN
                     EKLDTTAKAGLLADAYRAYMEAQTQRLVLPDLAQILHHVEQNDVLSADQKFALVEDMF
                     RVYFDDSRRKFILPDLSENLELIEKSTVFSEDQKRKLIDFQYSSYADAADRIHGMSNL
                     IRAYGLFERDVNYVVQDDKVVIVDEFTGRLQPGRRYSDGLHQALEAKENVTVERDTQT
                     VATITVQNYFRMYDKLAGMTGTAETEEDELWGIYKLEVIVIPTNKPVRRIDLDDVIFR
                     TKREKFNAVVEEVEQLHQQHLPVLIGTRSVETSELLSRMLKRKGIAHSVLNAKYHQIE
                     AEIIAKAGQPDAVTIATNMAGRGTDIKIPRSLIKCDECKLRNGLPESTITADGMTIKD
                     CLEKQPCGLHIIGTERHDARRIDRQLRGRSGRQGDPGASQFFLSLEDDLMRLFGSERI
                     ANIMTRLGAQEGEVIQSPLVTKAIDRNQRTIEANNFEIRKRLLDYDDVMNQQREVIYD
                     RRRFALENDDLTGEIRSLTMNAIDDIITRFAENENDGEGWDFGALNAELSKRFGVEAN
                     LRFGPEETVSDEVIRQRVTSAVLSKYDARCNELRREARRQGAEDEEVFVRRLHKIAVL
                     SAVDDRWREHLTDIEDMKGGIHLRQYGQKDPVVEFKREAYDIFENLIREIDRQTLEIV
                     FKSQLPSEREVEARRRAAAELMATHAEMEGFDAGEGAPVAVAEPEIRTVGGRGARKPV
                     TAARASAPKSTPQQAKAVPRVSRNSPCPCGSGKKYKYCHGKDET"
     gene            complement(20104..20589)
                     /locus_tag="PLT86_02500"
     CDS             complement(20104..20589)
                     /locus_tag="PLT86_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT35516.1"
                     /translation="MDFQPGKHYIGVGVGGIITGSDGRVLLARRSNAARNQRGQWENP
                     GGALEFGERFEDAIVREIHEELDIEVVPVGLLRVVNHIIPADHQHWVSPTFVCRHVRG
                     VPHINEPLKCSAAEWFALDDLPEPLTAISRTDLEYYKQVVESGRPLLPVTWCSPELPV
                     S"
     gene            20774..21994
                     /locus_tag="PLT86_02505"
     CDS             20774..21994
                     /locus_tag="PLT86_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35517.1"
                     /translation="MPHTPKFYPFSDVPVSDSCWSLSTGPDGRIYIAVCSEGLGGVSV
                     YIARYNDETDRLDYLVDASKETNDPPDSGRATQCKIHYCFCPSRHDGILYAATHLSGP
                     ALGDFLYSPWADWNDPRKAFRGSALMAFDTKTDTVLWWDTIAPREGGRCMALDENRGK
                     LYCITYPRDHFVIYDIKTRSRRDLGRIGAINSQIVFTDRKGRAFTSSDMGQLVRYDPE
                     RDRLEEIPAYLPCEPYQSRWHSVFYDAVASPEGDCIYGVTWREHMHLFRYWPEEGEFG
                     RMEDFGPVTQERDHSYPADTFSDHAGGLVFGADGMLYFVHSRWPEKSSFPTPEGAVGV
                     VCQVNPQTMEKKDFAYLKEEGRPSQYVSRAGRDRFGNLYFGHVGGTPSGMFRLPMDNP
                     SGRSDLHEPLRTWG"
     gene            22016..23209
                     /locus_tag="PLT86_02510"
     CDS             22016..23209
                     /locus_tag="PLT86_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35518.1"
                     /translation="MALDLSNKQWPVHPSKNPEFDLSSSHVKNIEKYHHRSFTQEGAM
                     VAMPLAFPGITTPIPADECAIISLGMDSTRMVFGGTAGRAAHVFAAMTRGVTGIAHDL
                     GVIPGATACTAVMPAPDRTIFLAASGSFGGSLYSHPPVPWPYDCFQEWGFSRPGYTEK
                     ARVFSDEGIACAVFDELRKTIYGVSDRTGTFFAVETDHQEWEVRTFGKVDAVHRFSRV
                     LVRDNGGNIWGTACNGSLWRFSPEDERLVDLDIWIPCAAGREIHNRAEAFALDPVTGI
                     IYGSGSSDGFLFAFDPEQQAVRSLGKPGVSPTIRALTVGNDGRLFGMCGGDDDIAHLF
                     VYDPADGTLRDIGTPVSLYGARTYGYVFASALTGKDGELFFGQAERMSHIWQYFPAIP
                     QRERA"
     gene            complement(23269..23673)
                     /gene="mutT"
                     /locus_tag="PLT86_02515"
     CDS             complement(23269..23673)
                     /gene="mutT"
                     /locus_tag="PLT86_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HOT35519.1"
                     /translation="MSSRPIEVVAAVIRRGGRVLVTQRNPDSHMGGLWEFPGGKVEPG
                     ETHAEALKRELREELGVRAVVGEQITSIRHAYPDRTVHLHFYRTELRDGDKPRPIGCA
                     DIRWVTLDELAALPMPPADAPLVCLLEGEPKT"
     gene            complement(23670..24287)
                     /locus_tag="PLT86_02520"
     CDS             complement(23670..24287)
                     /locus_tag="PLT86_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT35520.1"
                     /translation="MTPKRILFIGNSITIHPPKEEIGWFGHWGMAASAPEKDFVHLLL
                     KRFAEHAGGVEPEARVESGVAFERGYATFDIAGEFMPLSGFRADLVVLAIGENVGALD
                     TEEKQGMFGAATERLLKVVRHGYEPKMVVRSCFWADAAKDAVLRKVSDRIGGTFVDIG
                     HLGSVEANYARSEREYAHAGVAAHPGDAGMAAIADSIWNALHPAG"
     gene            complement(24642..26180)
                     /locus_tag="PLT86_02525"
     CDS             complement(24642..26180)
                     /locus_tag="PLT86_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT35521.1"
                     /translation="MLTHVFSAATHGVDAYIVDVEANLDSQVPSFTVVGLPEGAVKES
                     RDRVIAAVKNTGLYFPLRRITINLAPADIRKEGSAFDLPMAVGILAASEQAVSENLPH
                     AVLVGELSLDGSLRSVPGVLSMAVTAKREGKRMMIVPSDNVQEAALVEGLEVYAAKNL
                     DEALDILANPGGHVPHSLNLAEVFAHHVDDTVDMFDVKGQQHVKRALEVAAAGGHNVI
                     MIGPPGSGKTMLARCMPTILPDMTLDEALAVTKIHSVAGQMEPGQALIATRPFRSPHH
                     TISDAGLIGGGTYPKPGEVSLAHHGVLFLDELPEFKRTVLEVLRQPLEDGRVTISRAA
                     TSLDYPAEFLLVASCNPCPCGFFGDASGKCTCTPQMIERYMSKLSGPLLDRIDIHVNV
                     PAVPYKELAADPTGERSAAIRERVNRARDIQAQRFREREGLFCNARMVSRDLRTYCRI
                     DDQSHELLRMAIQKMGLSARAHDRILKVARTIADLDGSNQVTARHVAEAVQYRSLDRQ
                     LWLR"
     gene            complement(26352..>26752)
                     /locus_tag="PLT86_02530"
     CDS             complement(26352..>26752)
                     /locus_tag="PLT86_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35522.1"
                     /translation="VAAFVGMTRQEQIPRKESAEMMRRVTLEHDVRSLQEACEGIVPD
                     ERVKHWTVEKRSESWRRVFSDPAVCTCLAEEAGVAVGWISFGKSRDEDAVNCGEVYGL
                     HAHPDHWGHGIGGKELWALRYSTAVPARSN"
BASE COUNT         5177 a         8233 c         7573 g         5769 t
ORIGIN      
        1 atccgccgcc accaagaact cggaggttcg gatcgaagag tgatggcgtc ccgcaagaaa
       61 tctatccgat cccaggtcag gttcatgatc tcgctgcgtc gcataccagt gctcaaagcg
      121 aggaggacga tcgggtacaa gaacgaggaa ctgctttgct tgcacacgct cagaagtcgg
      181 ctccgttcct ggtcactgag gtaccgcact actccttggg gttccttgtt ccgcgatacc
      241 ttgcgcatgg ggttatcgtc taaccagttg aggtccctca ccgcgtacgt caagcaatgg
      301 ctcatggcag ccatgtaacg attgaccgta ctcggggaac ggggttttcc ggaaggagtc
      361 ggctctcggc tgagtccttc ccgggcatcg gccaggagtg cgggcctgag ttcctgtaga
      421 aggtagtgac cgaggcgctc tcgccaccac tccaattgac ttgcctgatt ccgagatgta
      481 gacaaccgct tcctgggtag tgcattcgcg atgtacttgc cgatcaactc cgcgaccgtg
      541 tggcggttcg actccggtgt cgggaagtcc cttccagcac gcatgtcggc ttccgtcctc
      601 tgtgcccatc gttcggcgtc tgtcttgcgg ccgaatgttt tggtcagaga cgggaatcct
      661 ctgaggcgaa cctggacgcg gtaatgcgtc ttctctgctt gatcagtgcg gggctggatc
      721 gttgccacga ggttgtgtcc ccctgccagt cttggatgaa ggtgcgcttc gttggaacct
      781 ccggtcttgg aaaaccgctc cggctacgcc agagcaaccc accatttggc gatgggctac
      841 gggcatgaag ctacgaaagt cggtgtgaac agacaatgag tgtattaccc ggaccgaggt
      901 cggcgttggt ccccctttaa cgatctacca cgcggtcgtg tccgtttgtc cgtgtttggc
      961 gcatcgaagg gccaagttgc aagcgagagg aacgggaaga cttggtgttg cggcttaccg
     1021 ctgaggacgc ggttgtgttg agaggtgttg cttctgagtg tgccaccagt gtgccatggc
     1081 ggcagtgtga acaaaaaaca gcgacctaca aatgcatgta agtcgctgtt ttataacaag
     1141 tagcggcgca gggacttgaa ccccggacac gcggattatg attccgctgc tctaaccagc
     1201 tgagctacgc cgccgaaaac aaatacctaa tctacgcaga atccgccatc cgggcaagct
     1261 ccgttacgtt cgtccgcgtg ccttccggag aaatgtcggc atccggaggt ccgcagaatc
     1321 cgtgcttccg gcgtaaatac cgtattgccg tgggagttcg cggtccagtg attcccgcgg
     1381 gaccgatgaa acgggagcta ccttcggttc tttgggaacc aggacgtgcg ctgctgttcc
     1441 cagagatgag ggttcttccg ccggggcttg gagaggctct tgttgtttcc gctgccaggc
     1501 gggtatctga agctcgttct ctgtcatcgg gatgttgacg accgtttcga ccgtattgcg
     1561 ctcgactttc tcctcaacgg gcaattcaac cggttccggc accgcttccg caaccggctc
     1621 tggctccggc tccctttccg gttccagtgt gttgaccgaa accgtcggag aggtcggtac
     1681 cgcgaaaggc ttctgcgcgg gagtcatgat ttccctggtg aacgtactcg ggcaacggct
     1741 ctcgaacccg gttgcgatga ctgtcacccg aatttcgcct tccggaacgc tgtcgtcgag
     1801 aaccaccccg aagaacacgt ccgcatcggc tccggcttcc cggttcacca ccgacatcgc
     1861 cgcgtttacg tcttcctgcg gcaacgacga attggaggtg atattcacca ggagtccttt
     1921 tgcgcctctg atcgagacgt cctcgagcaa ggggcttgcg atagcgatct ccgcggccct
     1981 gcttgcggcg ttttctccgg ttgccacgcc gcttcccatg gtggcgtcgc ctcgatggct
     2041 catcaccgtc cgcacgtcag cgaaatcgag attgatcaac cctggaacca ggattatgtc
     2101 ggtgattcct tgaacggcct ggagaagcac tttgtctgcc tcccggaacg catcgttgat
     2161 cggtgtcctg ggcggtagaa cctgcagcag gcgctcattg gaaatcacga taagtgtgtc
     2221 gaccgcctcc cgaagtgctt cgaggccttc tgccgctctg cggaggcgct tcggctgctc
     2281 aaatgcgaaa ggagtagtta ccacccctac cgtgagcgca cctatttcgc gcgctatctg
     2341 ggcaactacc ggcgatgctc ccgtgccggt tccgccgccc atcccggcgg tgatgaagac
     2401 catgtcagag ccacccaatg cgtcggcaac gcgatctctg tcctcgtcta tcgcctgtct
     2461 gccgatttct ggacggcccc ccgcgccgag ccctttggtc acgttgttgc cgatacagat
     2521 ctgggtggtt gcgcccgagc ggtggagcac ctgctggtcc gtgttgatgg cgatgaattc
     2581 aacgcgcgaa aggccactgt cgaccatcct tcgaatggcg ttgccgccac cacccccgac
     2641 accgatgacc ttgatccttg ccacgggttc ctgctcgtgg atcagttcga agttgtcatt
     2701 tgacatttct ccacctcctc tgctccgaaa ccctcgcggc aacccttccg tgcccgacca
     2761 ctccggcttc atcgcagttg ttttgggacg tttccgacga gtctactttc tatgcgaaga
     2821 attccttcag ccaacgacgc atgccctcaa gcggctttct ctgcatctcc tggttgagtc
     2881 tctcaagctc tctctcagcg tctgtcatgg acatcgtttg tggcgttttc aacaactggc
     2941 tggctccaaa tagaaccagt ccgactcccg tcgcgtttgc cggcgtcgcg acgctttccc
     3001 gtattccgcc aaccccgagc ggataaccga ttcggacggg gacgccgaaa acacgttgcg
     3061 cgagttccgc tacacccttg accatcgctc cgccgcccgt caccaccacc ccgctcgcca
     3121 ggtactcctg gtggccggat ttgcggatct cgaaccaggc agcccccagc aactccacca
     3181 tgcgcatctc gattattcgc gcaagttcga ccgagaactt tggttccggt attttcgcgt
     3241 cgaggactgc ccgcgtgttt tcgcggagga agcgtatatc aggttgttcc ctcggatctt
     3301 caggcaggtt gtgttcctgc tccgcgagtt gccgggaagc ccgtccatat tgctttttca
     3361 gctgttccgc ctcgctcatg aggatcgaac agccgattgc gatgtcgctc gtcacgtacg
     3421 cgctgccgat gcttaacact gccgtgtgac gtatgcatct ccggtggtac actgcgatat
     3481 ctgtcgtgcc gccaccgatg tccagcattg cgacgcccca ttcctgttca tcaggggata
     3541 gcacagccaa tgcgctcgca agtggctgaa tgactatgcc cgcgacctgg agccctgcgc
     3601 gttggaccgc gcgccgcaga ttctgttcgg cagtggcggc gccgcgtacg atgtggactt
     3661 ctccttcaag acgcgatccc gccagtccga tgggttgaat gatgtgcttt tgatcgtcca
     3721 cgcggtactc ctgcggaatc acatgaatga cggtcatatc cgcaggcatc gaaacgttct
     3781 gcgcatggtc gatgactctc ttgacatcct cttccgagat ctcgttcgtg atcttcccga
     3841 cggtgaccgc cgcgtggctg tttattccgc ggatgtgctc tcctccgatt ccgacgtaca
     3901 cggaggagat acggcacccg gccatatgtt gtgctcgttc cacggacttc tggatcgagt
     3961 ccacggtttc gtccagattg actatcatcc cgcgttccac accgcgggac tctgtctggc
     4021 cgacaccaat gacggacacc gcttccgatt catcgacctc tgcgatgatc gtggttactt
     4081 tcgtggtgcc gacatcaaga ccggcgacaa ttcgccccag tggcatcggt cagatcctca
     4141 tatcctgttg ccttacgacg acggagaaga tgtgacggcc aggtgcccga ggaaacgagc
     4201 atcaaggtgc gatgcggtca tgagaagatc cggttcatac gcaattaccg atgcaagcat
     4261 taccagcctg tctcttcccg ttaacctggg caacgaaaca ggtgtcccgc gcggcgagag
     4321 aatgagcacc atccgcgttg aatctgcgag agagagctct gaaaccgtgt ttcgcagcac
     4381 gggaaactcc gcgacgacgg cgagcgcccg ccctgccggt tccaggcttt cccgctgcgg
     4441 gaatggtacg gctttcaatc ccgttacgat aggcaggtcc ggcacggtgc gaccgttcag
     4501 tggcgttaca aagccctcct cgtccatgag gtaacagcga tccagctgga acaccgcgac
     4561 tggtgcgcgc tcgactacct cgacgcggac tgtgccgggc aaatggcgtg agactcgtac
     4621 gtcccgcacc catggaaccg atttgacgcc cgcagtgacg gcgttgacgt cgagcgcaag
     4681 aagaggcgaa ccacgtcgga tacccgccgc gtcgtaaatt gctcgcacgt ccacctggtc
     4741 cgttcccacg acgacaacat cgtcaatggc gagccaggga agacggatga ttccgtaacc
     4801 cgtaccccac aacgcagccc cggtggccac gtacgcgacg aatgccacca ggcgtgctat
     4861 ccagggccac cttgagaaca cccgagtttt gtgggtaacc agccccgccc agactcgctg
     4921 acggaggccg gtgccgcttt ctgacgttgt cggaggcgtt tcttccgcca tgtcagctgt
     4981 tctcctcggt ttcagccaga gcggtaccca ccttccagat gtcaccagcg ccgatggtca
     5041 gaacgatgtc gccggacctt gcagaatcgc ggagaatggc agcggcttct tcgtgggaat
     5101 ggacctcacg cacatccctc gcgcccgccc gcgagcacgc ctctgcgacc agtcggctgg
     5161 tcaccccctc gataggggtc tcgcgtgaag ggtagatgtc cagtaccagc acgagttctg
     5221 ccctcgcaag tgcggcgccg aactcctcgt acatgtcccg agtacggctg aacaggtggg
     5281 gctggaacgc caccagcaat cgcgcgcctt ctcccgccat gttacgtgcc gtgtccagcg
     5341 tgacacgaac tgccgtcggg tggtgtgcga agtcgtcaat gattgttgcc ccgtggacct
     5401 tccccctcac ctggaagcga cggtccacgc cagagaagcc cgccagtcct tcccgaatct
     5461 gttgcacgga aacgccgaga ttccgcgcga ctacgatcgc agcgagcgca ttctggacgt
     5521 tgtgccgacc aggtacggcc agtgctacct ctcccaagtt ctcaccgttt gcgacgaccg
     5581 agaaccggct tcctttctcc cgtgaacgga cgtcaattgc ccgtacatcc gcatcacccg
     5641 cgaacccgta cgtgaccacc ctcctgcgta tggaggggac gagcgcgcgc acattcggat
     5701 catctattcc gagtatggcg acgccgaaaa acggcagtcg ctcgagatag gctttgaacg
     5761 cgctctcgat atcgccgaaa tcgcgatagc agtcgaggtg atccgcctcg agagtggtga
     5821 tcacgccgat tgtggggaac atccgcaaaa acgtccgcgc gtactcgtcc gcttccacga
     5881 caaaatagcc gcctttgccc agaattccgc cagatcccag actcttcgtg atcccgccca
     5941 cgatgatcgt aggcgccatt cctgcgtgcg tcagaagctg accgaccatt gaagtcgtgg
     6001 tggttttccc gtgagttccc gcgattccga tggtgaacct catccgcgtc aactcgccca
     6061 gcatttccga ccgctcgatg accggaattt tccctgcctg agcggcgagc agctcggggt
     6121 tgtccgcctt tacggcagag gaatagacga ctacctgcgc gccccggaca ttggcggcgt
     6181 catgcccttc cgctacaggg atgccaattg attcaagacg tgccgtcacg ggactcaaaa
     6241 gcctgtcaga acccgtcacc gcgaacccga gagagtgcaa tacctcagcg attccactca
     6301 ttccgctccc gccgattccg accatgtgga tccggcggat tttgtggaac atcggaacca
     6361 cgctctcacc gttcattccg tgtcctcttt cacttcttcc tcgccttctc ccaagcggcg
     6421 ctggcgggaa aggttgagaa gaagtccggt cccggcaagg ttgagcacga gggaggaacc
     6481 tccgtaactg ataaatggca gcggtaaccc ggtggtcggc atcagtgccg tcattacgcc
     6541 catattcagc aacgcgtaca tcagaatgtt cgccgtgagg cccgttgcaa gcaggaatcg
     6601 aaaaagtgtc ggttgttggt aagcgattcg aagtccgcgc cagccgaaaa ggcaaaagag
     6661 cagaacaaca gcaaccgtgc ctatgaaccc aaattcctct ccgattatcg ggaggatcga
     6721 atccgtatac ggttccggca ggaagaagta tttctggatg cttctcccga ggcccctgcc
     6781 gagcaaaccg ccagaaccca tggcgacgaa gccctgctgg atctgatatc ccgtttcgag
     6841 ccgcttcagt ctgtacggct cgataattat caagacgtac acggcaagcg cgactgcacc
     6901 cattaaaggc agaatgtgtc gcatacgtat gttggcgacg aacatcatca ctccgaacac
     6961 ggaggccatt gccacggctg ttccgacatc tggttcgagc acaattatgc ccagcggtat
     7021 gcagatcagt ccgataagcg gtaacacgcc gtccttgagg ctttccagcc tttcctggtg
     7081 tttcgtcaaa cggtcagcaa ggtagagaat cagcgcaaat tttacgattt ccgacggttg
     7141 cagggtcaat cccagaaggt ttacccagct tcgcgcgccc ttgtatgcaa cactgaacag
     7201 ggccggcaac aaaaggagcg aaaaggacgc aaatgcaaaa tacgggaaat acttccgcac
     7261 gcgatccacc gggacaaaca gggcagcgat aaggcacaaa agtcctagtg ccgcgcgcgt
     7321 gacctgccgg accataaagt gcgccggccc accgaacttt tccgcggcga tgacgctgct
     7381 tgagctgtac accatcgttg cgccgaaacc gacgatcatg attgtggcgg ccagaaggat
     7441 gacgtcgagc ggctgagaag aactggccag ggcttgttcc gccccttcgg ctttgcccga
     7501 tccagccgct tctcggacga cctgtttgcg agaggcaccc gtgtcaccga ggcgttcgtg
     7561 cacccagttc agggaattcg cgtcgatgag tggtgcttcc acagacgttc tctccacggt
     7621 caagagagtg ctttctggag atggtctatc accaactcca tgtgctcgct gcgcgaacct
     7681 tttacgagaa cgacatcacc ttctctgagg ttattcctga gccactcggc gcacgcttgc
     7741 tgagtctcga atccgggtgt ttctgaagcg cccgcggatc gcgctccctg tgcaatgatg
     7801 ttgcccagtt cgccgacagt gatgaggttc acaccgagtc cggcgacgta cgagccgact
     7861 tcgcggtgaa gagcaaggct ctgttcaccc agttcacgca taccgcccag gacagctatt
     7921 cttcggccgc tctttcccgg ctcaagcgtc ataagggtct gcaaagcggc gcggacagag
     7981 gtgggattgg cattgtacgc gtcatccaac acgagaacgc cacgaatcca gagcgatctc
     8041 attctcctgt ccgccggttc ggtgcgagat atgccgacgg ctgcctcacc tgccggaatg
     8101 ccgagggcat cggcggcggc gatcgcagcg agcgcgttgt acaccatatg ccggccggga
     8161 attcgcaact ggacttccga gccgttgacg acaaagctcc agcacccatc ctggccctgc
     8221 tgcaaggcgg acgctttgaa cgcgcaatcg ctttcgattc caaaccaccg aatcgtcccg
     8281 tcccatcttc tcgcccgagc tacgagaccc acgtcgtcgc gattcagtac cagcatgcgg
     8341 tcacccgtaa gcgtaccgac cagttcccat ttcgcgtcca cgagagcatc aaatgagggg
     8401 aagctctcca ggtgggcctg tccggtgttc gtgatgacgc cgactgtcgc ttctgcgatc
     8461 gttccgagga gatggatctc gcccgcgtga ttcatgccca tttcgaccac tgcggcgcgg
     8521 tgttcctccg ttatctcaag aagcgtcagc ggaacgccaa ggtgattgtt aagcgtgcct
     8581 tcagttcgca gtacaggacc gagtcccgca agcgctgatg cgatgaactc cttggtggtc
     8641 gttttgccgt tcgagccagt cactgcgacc accggtatgg cgaatttcct tctgtgggca
     8701 cgtgccagtt tctgaagtgc ggcaaccggg tcatcgacgg tgatgagcgt atagatatcc
     8761 cattcgcgca gatgggcttc cacctttctc cgatctacca gtgcagctct cgcccctttc
     8821 gcgaacgcat ccccgacgaa acgatgcccg tcgttcgtct gcgtcgtaac agcgacgaag
     8881 agatctccct tgttgactcg gcggctgtca atcgagacgc cggttacggg tccctgactg
     8941 ccattgagca ccgtgccgcc agtgatctcg gctatctgtt ccgggctgta cgcgtccacg
     9001 ggatatgttt ccttccatta cggctttttg atggttttcc cggccaattc ctcgagagcg
     9061 gcgcgtgcct ccaatcggtc gtcgaagggg taccggacgc cctggatttc ctgatacggt
     9121 tcgtgcccct tgcccgcgat aacgacaatg tctcctcgcc gcgcaaccga aatcgcgtgc
     9181 ctgatcgctg ctttcctgga ttctatgcgt tcgaaagtgc gacctttcgg cacgccttcg
     9241 gaaatcgcgt ccaggatggc ctccgggtct tcagaacgag ggttgtccga ggtgaatacg
     9301 cagacgtccg cgatctccga agctattcgt cccataaccg gtcgtttgaa cgggtcgcga
     9361 tctccaccgc agccgacaac gcagatcacg cgtcccatgt ttcccgtcat ggaccgtgct
     9421 gccatcagga cgttttccag cgcatcaggt gtgtgggcat aatccacaat gaccgcgaag
     9481 tcttgacccg cctgaactgg ctcgaatcga cccggcgcgg gcgtagcgtc tttcaacccc
     9541 ctcgctattt cttccggcgc gagcccgagc gccagccccg ttccgattgc agccattgcg
     9601 ttggcgacgt tgaaccttcc cgcaagcggc atttccagcg tgatggttcc ctgcggcaac
     9661 ttcactctgc cttttatcca gggcaaatcc gccacttcca agggatggat atcggcagaa
     9721 ggcgagaatc cgtacgtaag aactttcgca gccgtgcgtc caacgagctt ttgaccatgt
     9781 cggtcgtcga tgttgattac tgcggtactc gacggtgcca gatcctcaaa aagacgcgcc
     9841 tttgcgcgga agtattcatc aagtgatccg tggaaatcca ggtggtccgt agtcaggttc
     9901 gtgaacaccg cgactctgaa gctcagaccc cagaccctgt cgagagccag gccgtgtgac
     9961 gatacctcca tcgccgtaag cgttgaaccg gcatcgttca gtgctttcag aagtcggcgc
    10021 agttcgtgcg cctctggtgt ggtgttccat gccgtgtcga acgggaagtc ctcaccgcgg
    10081 agagttgcac cgagcgttcc tatggtggaa ggaacaaagc caccggcagc gccgaccgag
    10141 ccaacaaggc gcacagttgt ggtttttcca ttagtgcctg tgacgcctac cagcgctgtg
    10201 ttcctgtcgg cgttcccgta ccaggcactg gccacgactg caagcgctct ccggacatcg
    10261 ggaaccacga tctgaggtac cgcaagcccc tcgattcgga ctggtgtaag cactgcggaa
    10321 gctccccgct cacacgcgtg cgcggcgaac ctccacccat cgacctttgt cccgggtaat
    10381 gcacagaaaa gggctccttc gcgagccgtg cgcgagtcag taacgatgca cgtcagcggc
    10441 cggtcacctg cactgccgct gaaaaaaagg ctgcccggaa cggtgtccgc aatggccttt
    10501 gcagtccacg gtgcacccgt cactggcttc gtccccgtcg gattatcggt gcgcctggtg
    10561 acgtcggctt tgacgccacg tcgcttgcgt cgatcatcac aacatccgcg tcatccacgc
    10621 tcgcctctgg ctcctggcgt tcctcagaag gagtttcccg accatctggt tccaacatcg
    10681 cgcccgctgt tccgagagct cgttggcatc gcagcacgac ggtgcagccc ggttcgggaa
    10741 gcgtacccgg cgcaggttcc tgagcaataa cgattccgct gccttcccat cggggaacaa
    10801 aacccgcggc tgagagggtt gctaccgcct gccgcaccgg cgttcccgtc acgtccggca
    10861 atacgttggc aagtccctca cgttcggcaa gcgtcatcaa cgccggtcgg ttcggcgaga
    10921 cacggcaatc cggcggtggc cattgcgaca ctaccacgga accacgaccg gaacggcgaa
    10981 ccggaatccc gagcgacgcc aatcgggcag tagcgccgat ttccgacatg ccgacaacct
    11041 ccggcattcg tgggtagacg gcatcagaag gtctttgcaa gacagccgcg tcgcggttga
    11101 tgatccgggt catgatgttt ttgaacacgg gtccggcgac cgtggccccg taatagctac
    11161 cctggggctt cgtgacagaa accacacaga cgagacgcgg gtccatgtcc gggaggaacc
    11221 cgatgaacga ggcgatgtag gcgccccgct cgtacccacg ccgcccggcc gccgccatct
    11281 gcgcggtccc ggtcttacct ccgacgcgta cgccggggat cgcggctttc ttcccagtac
    11341 ctttttcaac cacgccgcag agcatgttca ccatggaatc cgctactgcc gccggaatga
    11401 cctgtctcac ctttctgggt tcaaaagcgt ggatgatttt cccatcggga gtcgttattc
    11461 tttcgaccac gcggggcgcc atgagtactc cgccgttcac tatcgcaccg tacgccacgg
    11521 ccatttgcag cgcggtaaca cccacctcct gacccattgc gattgtcgca tgtgtccgcg
    11581 ctgaccagcg cgacggccgc gcaagtaatc cccgtgtctc accggggagg tccacgttcg
    11641 tcacggaacc gagcccgaac gcacgcatgt acccataaaa cgtctcggac gatatgcgct
    11701 gggaaatctt tatcgttcct acgttcgaag agtgtgcgat gacaccccgg acgtccagaa
    11761 cgccgttggg gtggctatcg cgaatcatcc ctccggcgac acttatacgt cccccctggc
    11821 aatcgatcat gtcgtccggt gtgacctttc cctcggccat cgccgcagac acggttatcg
    11881 ccttgaacat ggaaccgggt tcgtacgggt ccgtgatggc acggttccgt cgaagcgctc
    11941 cgttcctcgt ctgggaggac tcatcgggat tgtaggtagg cacgtttgcc attgctcgga
    12001 tcgcgccggt tgatgcttcg taaaggaccg ccaacccccc ggcagcatgc aaggaatcca
    12061 ccattttgga aagctcttgc tctacaatgg cctggtagtc ggcgtcaatg gtgagcgtga
    12121 ggtcgtttcc gtgcacaggt ggcataagcg gctgggccgt gttcgggact tgttctccaa
    12181 gcccgtccac aagcgaaatc atccacccgt ctttcccgcg caactgctgg ttgtagagcg
    12241 cctcgagacc ctcgcccccg acattgtcta tggaaacgtg ccccagaacc tgtccggcga
    12301 gtcccccgag cggatgtttt cttttggtga ccggatgaat cgtcatcgcg tggggtgcaa
    12361 gcgcgaatat ccgctgcgcc tgatccgttt caatattccg tgctacccac gcgaacgctc
    12421 gtccgggttt ggagattctc tggtatattc gctgtgccgg ctctccagat acttcggaca
    12481 ccctttccga aagcagtttc atctccgtga gtttgcgtgg gttcacggag acggaagctg
    12541 ctgcaagact taccgcaagg tcgcgcccgg ttcgatcggt aatcttccct cgcctcgcgg
    12601 caagggtaat ctgccgttct tgctgtccgg ccgccttcgc aaggtatttc tcatggtcaa
    12661 gaatctggat cttgacgagg cgactcgtga tcgcccccaa tccgaggagc cacagaattc
    12721 cgacaatcac cagccggatg gtgtgagccc tctgtgcagg gccgtcgtac ggtctgtatt
    12781 tcggttcgag aggcggggtt tgtccgggag cggttacgaa atcggggcga gggggattcg
    12841 cctccaccgg tgcggttctc agctctccgg gaaacggcag acccggccgt ttatggtcgg
    12901 tatccggcgg cggaacgcgc cgacggacgg ccgacgcctc gagtgcaaca tcccgttcca
    12961 gaccaggttc atgttgcgtt gttggcatcg gttccgcctt gtggtatccc gaaaagcatt
    13021 gtgtgcccta ctgcagccgc ggtttattgc cccgacgacg atcgagagcg catcgcacgc
    13081 aaaatctctg ttcctgttgc cctgcactcc gccaacgagt ccggagaccc gagcgacccg
    13141 attgcaggca cgtcgaccgg tggcgtcgtg ggctccacga gccccaaccg ttcgctcacg
    13201 atatccttga tccttgccgc gtgagagagg gcagtcgcgc gggcgcgcag cttgtcctgt
    13261 tcatccagca actccctccg ttccgcgcgc aattgctcca ggcgcagggc aagttggcgt
    13321 gagtgccaac ctttccacac gaatgcgatc atcgtcgcga cggccagcac cacgatggct
    13381 gcgccccaga acagattgtg agctctcaca atcgctccgc cacgcgcata cgcgcgcttc
    13441 tcgaacgcga attccccgcg atttcttctg ccgtcggcat gactgcccgg cgtgtaatgg
    13501 ccctcatccg gggaacgtgt ccgcaccggc attcaggcac ggtcggcgga caaatgcaac
    13561 cttgggtttc cattcgcatc catgccttca ccgacctgtc ttcgagtgag tgatacgaga
    13621 tcaccacgag tcttccgcca cgttcaagca ggtccggcac aagtcccagt acccggttca
    13681 atgaagccag ctcgtcgttc accgcgattc tgagcgcctg aaacacgcgc gaaagtgtct
    13741 tttccggatg tgttcctcgc cggcctatcg cctccacgag gtccgaggtg gttgccaaag
    13801 gccggcggct cctccgctgg aagatcgatc gggcgatgga tttggcgtgc ggctcctcgc
    13861 cgaaaacgcg aaagagacgc gcaagctccg gttctggcca ctcgttcaca atggtggccg
    13921 ccgcagtttc cgtgtcaggt cccatgcgca tatctatggg tccttccgac cgatagctga
    13981 atccgcgttc cggttggtcg agttgtcgcg acgacactcc caggtcaaac agcatcccgg
    14041 aaatgtgccc aagcttcgca ttttcaacga gttcatcgag ccgcgcaaac gatgcccgcg
    14101 cgaaaacgac gcggtcttta tactggttga ggcggtccgc cgcgcgttcg agagcctccg
    14161 gatccctgtc tatggccaga agccgaccgc ctccggccag ccgctcacaa atcttctctg
    14221 cgtgcccgcc gtcacctacg gttgcatcga cgtaggtgcc gtcatttcgc cagacaagtg
    14281 cctcgaccgc ctgatctgcc agcaccggca cgtgccaccg ataatgttcc ggcatacgga
    14341 tcaaccgctc tgtcggttct gacggaacga gacgaggttc cgcatattct gttcaaaaga
    14401 gttttcagcg gctgctatcg ccgcttcgta gcgatcaggg ttccagattt cgatccgata
    14461 ggtgacgccg acaaataacg cttccgacct gatgtcaact gctttcagca ggtacgccgg
    14521 gatggaaatg cgaccttgac catccagcct ggcttcgcac gcgtgagacg tcattacgcg
    14581 gatttcgttg cgcttctcaa acgaccgtgc atcgttaagc gcgagctccc actgctccgc
    14641 gagattttgc cacgtgtcga gaggatacgc gaaaaggcac ccgtccaacc cgcgcgtgac
    14701 gtaaagagtc ccctgcgact caggaagcat ggctttgcgg atggcacgag ggacctggat
    14761 ccgtccctgc gcatccacga cgtgacggta gttgccgacg aagctggcct gtcgccgaac
    14821 aactccgagt ggaccggttg ccatgagttt gtcctgttca caggggtggg gcagttcggg
    14881 ttgccggatg tgcgcgcccc ttatgggaac cgaattcagc caattctccc ctaaatgccc
    14941 ctgctcacca ctaggatatg cacgcaaata gtttccgtca agatgctaag gagttttttt
    15001 ttgcctcagg aaggagtgtt ctgtttttaa tctttaaaaa acaacgtatt acaggctcaa
    15061 gtgatttttc ggattcgttg ctcggatagg aagggaggag cggcaggatc gatgtttaag
    15121 aggcggaggg gagggagcgg cggccagtga tcgaggaatc aggaggatgt ccttttctgg
    15181 tccgcctgaa gacgggcaat atcgcgctcc agtgcggcaa tccggcggag caggtgaagg
    15241 atgtacaata ccgtcccgag ccacacagcc ccgtacgcgg ccactacgaa gtgcgcgttt
    15301 ctccacaggg tgacatcgcc caccagagcc tcccccgccc acgcctgacc cgaggcgaat
    15361 acaacacaga aagagagcaa caaaaaggcg gccactaggc gcagtcgggc gtgaaaatgc
    15421 ctctccatcg gaatactcct gtctttttca gcctgcgccg caccgcaggt aatcaacctg
    15481 ttccgtcgcg cgaagaacgc gatacctcag cgacagcatc actccaaaaa gcaccgtaaa
    15541 tgtcgcaagg gaaacgacta gagccaccag aaaatcggca ggaagcccgc tcgcaaaccc
    15601 ccgttgagac aggaccaccg cctccgggtg gagacctcgc cacagcacaa cggaaaaatg
    15661 gatcactggt acgtcaagtg cccctaaaag ccccagaacg gcacaaaatc gcgcggcctt
    15721 ctccggttct tcgacgaatt cccgcagcag aaggtaggct atttcaatga gccagagcac
    15781 gaaggtagtc gtaaggcggg cgtcccagac ccagtacgca ccccaggccg gtcgtgccca
    15841 aagaggcccc gttacgagca caagggtcgt gaatactgtt ccgacttcaa tggaggcgtg
    15901 tgctagatga tcccagcgtc tggaacgtct taacaggtag gctactcctg agccacttcc
    15961 gatgaaaaag gcgaggtatg tgatccacgc gcacggcaca tggaaataga aaatcttctg
    16021 cgtgaatccc agttctgcat cgagcggggt caccacgaag atcagataaa gggaaatcgg
    16081 aaagccgact gcagcaagga tcgtcaaggc cgtttggggt ctcgacaacc cggtctttgt
    16141 ggtcaagtct cgtcctttcc atggcaatac ttgtacttct tgccactccc gcacgggcac
    16201 ggcgaattgc ggctcacgcg aggtacggcc ttcgcctgct gcggcgtaga tttgggcgca
    16261 gaagcacggg ccgcggttac cggcttccgt gcgccacgcc cgcccacggt gcggatttca
    16321 ggttcggcga cggccactgg cgcgccctct cccgcatcaa acccttccat ctcagcgtgg
    16381 gtggccatca actccgcagc ggcgcgtctg cgtgcttcca cttcgcgttc tgagggaagt
    16441 tgtgacttga acactatttc cagcgtctgc cggtcgattt cgcggatgag gttttcgaat
    16501 atgtcgtacg cctcacgttt gaattcgacc acggggtctt tctgaccata ctggcgaagg
    16561 tgaattccgc ctttcatgtc ctcgatgtcg gtgaggtgtt cgcgccagcg gtcgtcaaca
    16621 gccgacaaga ccgcgatttt gtggagtcgg cgcacaaaaa cctcttcgtc ctccgcacct
    16681 tgtctacggg cctcgcgccg aagctcgttg cagcgggcat cgtactttga gagaaccgcg
    16741 cttgtcaccc tctgtcggat cacctcatca gagactgttt cctctggtcc aaaacggagg
    16801 tttgcctcta ccccgaatct cttgctcagt tccgcattga gcgcaccaaa atcccagccc
    16861 tctccatcgt tctcgttttc ggcaaatcgg gtgatgatgt catcaatcgc gttcatggtc
    16921 agcgaccgga tctcgccggt caaatcgtca ttctccagag caaaccggcg gcggtcataa
    16981 atgacttcgc gctgctggtt catcacatca tcgtaatcca gcagccgctt tcttatctcg
    17041 aaattgtttg cctcgatcgt acgctggttt cgatcgatgg ctttcgtcac cagcggggat
    17101 tggattacct cgccttcctg agcaccgagt cgcgtcatga tattcgcgat gcgctccgag
    17161 ccaaaaaggc gcatgaggtc atcttccagc gaaaggaaga actgcgaagc acccggatca
    17221 ccttgtcgcc cggatctgcc tctgagctgg cggtcgatac ggcgcgcgtc gtgccgttcc
    17281 gttccgatga tgtgcaagcc acagggttgt ttttccagac agtctttgat cgtcatgccg
    17341 tcagcagtga ttgtgctttc aggaaggccg ttgcgaagct tgcactcgtc gcatttgatg
    17401 aggcttctcg gaatcttgat gtcggtgcct cgtcccgcca tgttcgtagc gattgtcacc
    17461 gcgtcgggct gcccggcctt cgcaatgatt tccgcttcga tctggtggta tttggcgttc
    17521 aaaacggagt gggcgatgcc tttgcgcttc aacatcctgc tgagaagctc gctcgtttcg
    17581 acggatcgcg tcccgatgag cactggcaga tgctgctggt gcagttgttc tacttcttcc
    17641 acaacggcgt tgaacttctc acgctttgtg cggaagatca cgtcgtccag atcgattcgc
    17701 ctcacaggtt tgttcgtcgg gatgacgatg acttccagct tgtagattcc ccacaactca
    17761 tcttcttcgg tttccgcggt acccgtcata cccgcgagtt tgtcgtacat gcggaagtaa
    17821 ttctggaccg taatggtggc cacggtctgc gtgtcacgtt ccaccgtgac gttttctttc
    17881 gcttcaagag cctgatgcag tccgtcggaa taccgtcgcc ccggttgaag ccttcccgta
    17941 aactcgtcga cgatgacgac cttgtcatcc tgaaccacgt agttcacgtc gcgttcgaac
    18001 agcccgtatg cacggatgag attggacatt ccgtggatgc ggtctgccgc atccgcatag
    18061 ctcgaatact ggaaatcgat gagcttcctc ttctggtcct ctgagaaaac ggtgctcttc
    18121 tcgatcagtt ccaggttctc gctgagatcg ggaaggatga acttgcgccg ggaatcgtcg
    18181 aaatacacgc gaaacatgtc ttcgaccagt gcgaatttct gatccgccga gaggacgtca
    18241 ttctgttcaa catggtgcag gatctgggca agatcgggca gaacaagtct ctgggtctgc
    18301 gcctccatgt acgcgcgata tgcatcggcc agcagccccg ctttcgcagt agtgtccagc
    18361 ttttcgttgt gagtgacgaa cgccgtcacg tcgtcgaaag atggccgcga aagcccttct
    18421 cgttccgctg tggagaatgc atcggaaaga gccttggtga ggtccccttt gtcctggctc
    18481 gtcaagtact cattcgccga gattcgttcc tcgatttctc ttccagggta cgagagacgc
    18541 ttgatggcct gttttaccca gttcgatgcg gactcgacga tttttgcgcg tccggcgtcg
    18601 cggtccgctt gcgaatcgtc ttccggcccc tcggcctcgt aggtcagcga gaatcgaatg
    18661 aacccgcggg acgcgtagtc cacggccagt gcaaactgtt ccttgagcag cacgcgcttc
    18721 tgctcgtcgg tgagtacctc atcgagttca atcgcacgaa cgacgtcgcc agcttctgac
    18781 gtgatcgctc gccccttcgt ctcgttgatg tacgcacgga attccttctc gaggcgctcc
    18841 tgtttgaccg cgtcgtcaac gttctcatcg cgttcgatgc ggcggacggt gtccatgagc
    18901 gaaatgaagc gtcttttgtc ctcggtgagc agaaggcggg agcggccctc gtcagtgatc
    18961 tccgccgaat gatttttctc gtcgatcgaa tagagcaacg gttcgtcgag ttcgggagtg
    19021 cgcttatcgc gcatgtaatc gagttctacg cggacgatcg cctttttcac ctcgccgctc
    19081 tcgcagagcc gcatgagttt tcgttgtttc ggagcgccgc gttgagcctg cagcagcttc
    19141 tttgcgccgg cgtacgtttc tcccgcttcc cacagccgct cgccgtcgtc gaccagttcc
    19201 gcgactatcc ggctctgttc ctttaccagc ttctccacgc gaggcttaag ttccacgtaa
    19261 cgttcgcttt tggactccgg tacctgcccg ctgatgatga gcggcgtccg tgcctcgtcg
    19321 ataagaagct ggtcagcctc gtccacgatc gcgaaattga agccccgatg acgaatgtcg
    19381 gcaatatccc acgccatatt gtcgtagaga tagtcaaatc cgaactgatc ctgcctgccg
    19441 taggtcacat cacaggcgta agcttcccgg cgagagatcg gcacaaggtg gtacccctgc
    19501 gtttcattcc aatcgacttt gtaactctca atgccgttgt tcgcgttcgg atcctgaaga
    19561 acggaaaccg agagcccgag ccagtggtaa atgggaccat tccagcttgc gtcgcgccgg
    19621 gcgaggtagt cattcacggt gacgaggtgc gcaccttttc cgggaagcgc gttgaggtag
    19681 agcggcaata cggcgacgag agtcttgcct tctccggtcg ccatttccgc gattttgcct
    19741 tgatgcagaa cgatggcgcc caccagctga acatcaaacg gcaccatgaa ccacggagtc
    19801 atgttcccgc agacatccca ttccatcttc cgttcagtga aacggcgcat cgtctctttg
    19861 acaacggcga acgcctcgac catgagatcg tccagggtct cgccgtccgc caggcgcttt
    19921 ctgaattcgt cggtttttcc tttgattgcc tcatccggca gatcatgaag caactcgtac
    19981 ttcgcgttta tctcggcgac gacaggccga agacgcttga tttcgcgttt gctcttgttg
    20041 aaaaactttt tgaacaaatt cacagcaaca ctcccgtttg tatcggcgat cgagagaacg
    20101 tcatcatgag acaggtaact ccggggagca ccaggttact ggcagcagtg ggcgaccgga
    20161 ttcgacaact tgtttgtaat actcgagatc agtacgcgag atcgcggtca gcggctcagg
    20221 cagatcatcc aacgcgaacc attccgccgc actgcatttc agcggttcgt tgatgtgagg
    20281 gacaccacga acatgccggc acacgaatgt aggggaaacc cagtgctgat ggtccgccgg
    20341 aatgatgtga ttgacaacgc gcagaagccc taccggcaca acctctatgt caagttcttc
    20401 gtgaatttcg cggacgatcg cgtcttcaaa gcgctctcca aattccagcg cacccccggg
    20461 gttctcccat tgacctctct gattgcgagc ggcgttgctc cttcgggcaa gcagaacccg
    20521 cccgtcagag cccgtaatga tcccgcccac tcccacgcct atgtagtgct tccccggttg
    20581 aaaatccacg ccacatttct ccgtccaaca aatgacaacc cattaaatct acgcccgtac
    20641 ccgcaggttc tcaagcataa gccgttcctc cgggctttgg tattgagacg aaggcgccta
    20701 taatcttcat tgttcgcgct gagtcgggga cctcctcccg ccttgcgcct caccacactc
    20761 caaggagata gcgatgcctc acactccgaa gttttacccg ttttcggacg tgccggtatc
    20821 agactcgtgt tggtccctct caacagggcc cgacgggcgc atctacatcg cggtctgttc
    20881 cgaggggctg ggcggtgtaa gtgtctacat cgcccggtac aatgatgaaa cggatcgcct
    20941 cgactacctt gtggacgcct cgaaggagac aaacgacccg cccgattccg ggcgagcgac
    21001 ccagtgcaaa atccactact gcttttgccc ttccaggcac gacggcattc tttacgcggc
    21061 aacccatctc agcggcccgg cgctcggcga cttcctttat tcgccctggg ctgactggaa
    21121 tgatccgcgg aaagcatttc gtgggtcagc cctgatggcc ttcgatacca agaccgacac
    21181 ggttctctgg tgggacacta ttgcgccccg cgagggaggt cgttgcatgg cgttggatga
    21241 gaatcgcgga aagctctatt gcatcaccta tccgcgcgat cattttgtca tctacgacat
    21301 caagacacgt tcccggcgcg accttggcag gattggcgcg atcaattctc agatcgtttt
    21361 cacggacaga aaggggcggg cgttcacgtc ctctgatatg gggcaactgg tgcgttacga
    21421 ccccgaacga gaccggctcg aggagatccc ggcttatctc ccgtgcgagc cgtatcaatc
    21481 acgctggcat tcggtgttct atgatgccgt tgcttcgccg gaaggggact gcatctacgg
    21541 ggtgacgtgg cgggagcata tgcatttgtt ccgttactgg ccggaagagg gggaattcgg
    21601 ccgcatggag gacttcgggc cggtaacgca ggaacgcgac cactcttacc ctgcggatac
    21661 cttctccgac catgccggcg ggctcgtgtt tggcgcggac ggcatgctct acttcgtgca
    21721 ttcccgctgg ccggaaaaat cgtcctttcc cacgccggag ggcgcggtag gcgtcgtctg
    21781 tcaggtgaac ccgcagacga tggagaaaaa ggactttgcg tatctgaagg aagaggggcg
    21841 cccgtcccag tacgtttccc gcgcgggcag agacaggttc ggcaatctgt atttcggcca
    21901 tgtgggcggc acgccttcgg gtatgttccg gctgccgatg gacaatccgt cggggcgaag
    21961 cgatttgcac gagccgttga ggacgtgggg ctgacaattc caaggagcga caccgatggc
    22021 acttgatctc agcaacaagc agtggcccgt gcatccgtcg aaaaacccgg aattcgacct
    22081 cagctcgagc cacgtgaaga acattgagaa ataccaccat cgcagtttca cgcaggaagg
    22141 agcgatggtg gcgatgccgc tggcatttcc cgggatcact acgccgattc cggcggatga
    22201 atgcgccatc atcagcctcg gaatggattc cacgcggatg gttttcggtg gaacagccgg
    22261 gcgggcggcc catgtgttcg ccgccatgac ccgtggcgtc acaggcatcg ctcacgatct
    22321 gggagtaata cctggcgcga cggcctgcac ggctgtcatg ccggcacccg accgaaccat
    22381 ttttctcgct gccagcggtt cgttcggagg cagtctctac agccatccgc cagtgccgtg
    22441 gccgtacgat tgtttccagg aatggggttt ctcacgtccc ggatacacgg aaaaggcacg
    22501 cgtgttttcg gacgagggga tcgcctgcgc agtcttcgat gaactccgca agacgattta
    22561 cggcgtctcc gaccgcactg gtaccttctt cgccgtggaa acagaccacc aggaatggga
    22621 agtgcgcacc ttcggcaaag tggatgcggt gcatcggttt tcccgggtgc tggtgcgcga
    22681 taacgggggc aatatctggg gcaccgcctg caatggctcg ctctggcgtt tttccccgga
    22741 agacgaaaga cttgtggatc tcgatatctg gattccctgc gcggccggga gggagatcca
    22801 taaccgggcg gaggcgtttg cgctggatcc ggtaaccgga atcatttacg gcagcgggag
    22861 ttcagacggg ttcctgttcg ccttcgatcc ggagcaacag gctgttcgtt ctctcgggaa
    22921 gccgggggta tcgccgacaa tccgcgccct caccgtgggg aatgatggaa ggctcttcgg
    22981 tatgtgcggc ggcgacgatg acattgctca tctgttcgtg tacgatcctg ccgacggaac
    23041 tctgcgcgat attggtaccc ccgtttctct ttatggcgcg cgaacgtacg gttatgtctt
    23101 cgcgtccgcg ctgaccggca aagacggcga gttgtttttc gggcaggccg aacgcatgag
    23161 ccacatctgg cagtacttcc ccgcaattcc gcagagggaa agagcgtagg gtcgccgcgg
    23221 cggtgtgggg gttccggtga ggttttgggg gcagttcgtg aactggccct acgtttttgg
    23281 ttcgccctcc aacagacaga ccagaggcgc atcagcgggc ggcatgggaa gggcagccaa
    23341 ttcgtccaac gtcacccacc ggatatctgc gcacccgata gggcggggtt tgtctccgtc
    23401 ccgcagttct gtgcggtaga agtgcaggtg gaccgtgcga tcggggtacg cgtggcggat
    23461 gctggtgatc tgctccccga caaccgcgcg gacgcccaat tcctcccgca gttccctctt
    23521 taacgcctca gcgtgagtct cgcccggttc cacctttccg ccgggaaact cccagaggcc
    23581 acccatgtgg gaatcagggt tgcgctgcgt gacgagaacc ctgccacccc gccgaattac
    23641 cgcggcgacg acttcaatcg ggcggctact catccggcgg gatgcaacgc gttccagatg
    23701 gaatcggcga tggcggccat ccctgcatcg cccgggtggg ccgcaacccc ggcgtgagcg
    23761 tactcgcgct ccgaccgagc gtaattcgcc tcaacgcttc caaggtgacc gatatccaca
    23821 aaggttcccc cgatcctgtc ggagactttg cgcaacaccg cgtctttcgc cgcatcggcc
    23881 cagaaacagc tccgcacgac catctttggc tcgtacccgt gcctgaccac tttcagcaac
    23941 cgctccgtgg ccgcaccgaa cataccctgc ttctcttcgg tatcaagggc ccccacgttt
    24001 tccccgatcg ccagaactac aaggtcggcc cggaatccgg acagcggcat gaattccccc
    24061 gcgatatcga acgttgcgta cccgcgttca aaagcaactc cgctctccac acgcgcctcc
    24121 ggctctactc ctcccgcatg ttccgcgaac cgttttagaa ggagatggac gaagtccttc
    24181 tccggcgcgc tggccgccat gccccagtgc ccgaaccatc cgatctcctc tttgggaggg
    24241 tggatggtga tgctgttgcc gatgaaaagg atgcgttttg gtgtcatgtg tggctccgtc
    24301 gcgtgtggac gattaccgtc gccgtctgtg atcattttgt gcgtcgtccg cagcgcggac
    24361 gcgcggcgaa aatctccatt tgtgaacaaa cccgtgtagc acgggctgcg ccctgcgcag
    24421 caggggtccc gcccgtgcac gaatcgctgc gactactata catgcatggg aaagatgtcc
    24481 atggtacggc cagccgccgg gccgggcaac cgggccccct gtgcccgaac gcaacgttcg
    24541 tcattcccgc gcaggcggga atccagagag gagcgcgcag ttgcgcaacg catatgtcgg
    24601 cactcccgac atcggctagg tggcccggca cgcagagcgc cctacctcaa ccacaactgg
    24661 cggtcgagac ttcggtactg cactgcctcc gcgacgtggc gcgccgtcac ctgattcgac
    24721 ccgtcgaggt cggcaatggt gcgggcaacc ttgaggatgc ggtcgtgcgc gcgggccgaa
    24781 agccccattt tctggattgc catacgcaag agctcgtggc tctgatcgtc aatacggcaa
    24841 tacgtgcgca ggtcgcggct caccatgcgg gcgttgcaaa agagtccctc ccgttcacgg
    24901 aagcgctgcg cctgaatatc ccgcgcccgg ttgacccgtt cccggatcgc ggctgatcgc
    24961 tccccggtcg ggtccgcggc aagctctttg tacggcacag ccggcacatt cacgtggatg
    25021 tcaatgcggt caagcaacgg gcctgagagc ttgctcatgt agcgttcgat catctgcggc
    25081 gtgcaggtgc attttccgct ggcgtcgccg aaaaacccgc acgggcaggg attgcacgac
    25141 gcgacgagaa ggaactcggc gggatagtcg agcgaagtgg ccgcgcggga aatggtgacg
    25201 cggccgtcct caagcggttg gcgcagcacc tccagcacgg tgcgcttgaa ctccggcaac
    25261 tcgtccagaa aaagcactcc gtggtgtgcc agactcacct ctcccggctt tgggtaggtc
    25321 ccgcctccga tcaaccccgc gtcggagatt gtatggtggg gagatcggaa cggccgtgtg
    25381 gcgatgagtg cctgccctgg ttccatctgt cctgccacgg aatggatttt cgtgacggcg
    25441 agcgcttcgt ccagcgtcat gtcggggagg atcgtcggca tgcatcgggc aagcatggtt
    25501 ttcccggatc cgggcggccc gatcatgata acgttatgcc ctccggccgc cgctacctca
    25561 agcgcacgct tgacgtgctg ctggcccttc acgtcgaaca tgtccaccgt gtcgtccacg
    25621 tgatgggcaa acacctccgc gaggttcagg gagtggggaa cgtgcccccc cggatttgcc
    25681 aggatatcca gcgcctcgtc gaggttcttt gcggcataca cctcgagtcc ttccaccaga
    25741 gccgcttcct gaacattgtc ggaaggaacg atcatcatgc gcttgccttc acgcttcgcg
    25801 gttaccgcca tggaaagcac ccccggaacc gatcgcagtg agccgtcgag ggaaagctcg
    25861 ccaacaagta cggcgtgggg gaggttttcg cttaccgcct gctcgctcgc ggcgaggatt
    25921 ccgacggcca tgggaaggtc gaaggcactc ccttctttgc ggatgtcagc gggagcaagg
    25981 ttgatggtga tgcggcggag tgggaaatag aggcccgtgt ttttcaccgc ggcgataaca
    26041 cgatcccggc tctctttcac cgcgccttcc gggagtccta ccacggtgaa cgagggcacc
    26101 tgcgagtcga gattcgcctc cacgtcaacg atgtacgcgt cgacgccgtg tgttgctgcg
    26161 gaaaacacgt gagtcaacat gaaacgtact tcctcggctt atgtagaacc gaaattgtca
    26221 gtctctaccg gaacattcat tatacgagtg gtgtgtgtgt gacgcgtagt gggggttggg
    26281 aaacgacagc agatcacgcg gcgaattggc cgggaggatg ccaacggggg aaaatgatgg
    26341 attcggactc gtcagttact tcgggcaggg actgccgttg aataccgaag cgcccacaac
    26401 tccttgccgc cgatgccatg accccagtgg tcgggatgcg catgaaggcc atacacctct
    26461 ccgcagttga ccgcgtcctc gtccctgctt tttccgaacg aaatccaacc gaccgctacc
    26521 ccggcttctt ccgccaggca cgtgcatact gcgggatcgg aaaacactct gcgccagctt
    26581 tcagaccgtt tttcgacagt ccaatgctta acacgctcat ccggaacgat cccttcgcaa
    26641 gcctcctgca aggaacggac atcgtgctcg agggtgaccc gcctcatcat ttccgcgctc
    26701 tccttccgtg ggatttgctc ctggcgcgtc attcccacga aggcggcaac cg
//
LOCUS       DAOSOO010000018        25923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1896_length_25923_cov_6.926318, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000018 DAOSOO010000000
VERSION     DAOSOO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 25923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25923
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_25923_cov_6.926318"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLT86_02535"
     CDS             <1..1029
                     /locus_tag="PLT86_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOT35523.1"
                     /translation="HVLSHCHASLHGSRHVVSVAPTDITGWREGPVVAQSISYPRVVR
                     LPGGRMLLYHRVLGHMGYWTYQTSNDGGFTWKRRATPLVDFDQRPEAPGDEWAGSYHS
                     VAPGKDGESLHIAFVYWDERKRVHPVYGIRLAHMDRYHLYYARLDIASGRLYSAAGKR
                     LGCPVNRREAEKCLVWDTGTRLTNMPSILVERDDRPAFLMPVTGERVDDCAFWCIRRE
                     QGAWQRFRVTDTNSTWAGSHLEADGAGGISAFLVGRPGRPGRLPYGGGTLQEWRSPDR
                     GESWEHIGDIVPKAGLVCNNPKPVETAAGEVLRRTLVFFGWEGPDGLAPEGPYGGQAF
                     LWRDGEWL"
     gene            complement(1189..1262)
                     /locus_tag="PLT86_02540"
     tRNA            complement(1189..1262)
                     /locus_tag="PLT86_02540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1226..1228),aa:Arg,seq:tcg)
     gene            1725..5285
                     /locus_tag="PLT86_02545"
     CDS             1725..5285
                     /locus_tag="PLT86_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT35524.1"
                     /translation="MPRSMSSYQRWFVAAGIAFLSAVSLLRAGTTGKITGIVRDQSGQ
                     PLPGVAVQISGLRLGGVTDADGRYFILQVPPGTHEVQANMVGYRTSITRDVLVGADRT
                     TTLNFTLREETIEVEAVVVTAGRPPIEVDVTSSQTTVNSARVAEVPVNQMLDVLAYEP
                     GVSIARDNELNIRGGGATQIRFQVDGLDRTDGLTNKPYTQLNQTLVAEVTLLTGGFNA
                     EYGNVRSGMVNVVTKAGNERGPNRGWVSGVYSMTPTARKKHFGPGGYSEDQFDYRTAL
                     LSDANTVGATGAADPYGAIYWPNLYESTRTDTAFRRVWTAAPNTYKVFDGWASRVTNA
                     NATNRGAGVYGKGTRTKPWTADQIIEAWRYEANLNENVWGYSHEPDWSLDLSAGWGLP
                     RKLGGIVIGYVTNKEMTITPALRPYYRDETFDVKLTLTPVDRLKWSFSYMHGTGVSTG
                     NGTSSYDPEMAETAGSIGIGGDAVSLRTAGDLLGTVYMSKGSYGNNRLNLSYNSFLDA
                     TFWQWGTALTYTISPATFATAAFGQTHTEYELLRDWPRADISDFSTTGTYKPSSTFSY
                     RAWLQMFALSWSDVNGDGRGDLPNDLNDALTPGRAFLEYPYGFPSAYKSVPSQTVYVN
                     RQFVFAPGDTARVVSPQGWVQEGYYDLSGTFWLGEGGQQYMTSHAVQTAARADITHAM
                     GSHTLKTGAEYLLADLEYHAEEASRIIGSRSNSNYRDYGGKFPASQPSILGIFLQDKF
                     ESQGMVMNFGLRAERFDGGAPVFMPDMIYDTRLFSPNNGKYWFDSLCVANGWNPSDPA
                     WGALPGSIGEAWSKFEKNGILDKFPMPYDVVAALPQRPAKAHWRLAPRFGISHPVSVR
                     TKFFFNYGIFYSMAKPAQMYGLGEHDGRTGALGRFEQLYYPELRPAKTTMYEVGFEQV
                     LPFSFVFKTTGYAKYNVDQVTGVQIHAGATDSYRTYRNANYQDIRGLEMKLARNGRFV
                     SGWATYQIIATRSGQTGLTDLYQNPTMNVYYTPLVTTNSPANNIAASVRVGTPVEWGL
                     LTGGWGASIIGRYNENTGEVIYNPNNQPRRELPPEWIMRGRDYWGADLKFTKDVAIGR
                     QRRASVYLDVTNFLNRKYLNGTYMNGNDYLAYVVERRAQGEKDLRVGDPSTWDALTQP
                     YRIRKADGTYTQWKAPISPRTDWIMFLYPRAYRAGIRFDL"
     gene            5326..7659
                     /locus_tag="PLT86_02550"
     CDS             5326..7659
                     /locus_tag="PLT86_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT35525.1"
                     /translation="MTATMRRWFTWAGLMGALVLTFSSTVMANTPQSVMHLGRIWHHP
                     EYDGAEGWSGSGPIYPAGIPTAADGDNSVKRGWIGQVQKFGVYLTTTDWTSPLNTALE
                     YTSSYCFRSMDYAYYRVYVTEKGDYNFPVGCQEVFRWTRPTIVAGDSTLKFFPGPGPA
                     AYPDNSGVGPRPTPVVDPTLVTEEAIDVTWRFSQGVEYRRRQYGYGITTAHQDYVLFD
                     TRFVNNGKCYGDTVLADPAPFTMTGQTIHKMIFGQAYDYRNNHAPGAKQVGKDNEGMY
                     IQPWGASLNSVALFYDLDSDDAAAPGPDWGDPSENAAMEGHLTGNAYMAIGPVFASKG
                     PGSLYGTNDPDQPAFRMMHNERGLDFAGKAYSPANSQELREFLTDGALQMPIDQSFRT
                     YAPTAAIKDEDPGATAILGYGPLGGDKALSPANVKVHGWDLAFGDSVRLVQVVAGGGI
                     DAYEGQRIGQYWNAQKAASAAPDTWMSAADQALVQTGMDTVKKALALAYWNFYGSFPA
                     NVDAAKKAAWGIPNYVNSKPAAYNQPFNVPDPPRPPAFFSVKALAKGGIVVRWTRESE
                     SAPDFDTGVNDFAGYRVYRWEASRLTKPVIVKQGPASSFWAVAQDEAGPGPAGFAYVD
                     DQVTTGTDYWYAVTAYDDGTQNWAEPGKSLESAYFWCWTGYTSKGVTAPTLNGQGVDV
                     ARANRFALEQNAPNPFNPSTTIRFAVPANGNVRLTIYDVNGRIVRTLVDGSVLAGSHE
                     VVWDGMDMTGRAVASGVYIYRLTTANNEMTKRMTLVR"
     gene            7892..8317
                     /locus_tag="PLT86_02555"
     CDS             7892..8317
                     /locus_tag="PLT86_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35526.1"
                     /translation="MNTRLRHWWIAGVAFAFVLIALPRVSHSAVSTLDSVIVIPNPYN
                     LSGRTYGPQPPGGSIEANERIRFANLPAPCTIKVYTSAGNLVATINHTGANTAVSDLE
                     NYYWSGRNADNQYIVSDVYIYVVESPTLGRKIGKFIVIR"
     gene            8455..9543
                     /locus_tag="PLT86_02560"
     CDS             8455..9543
                     /locus_tag="PLT86_02560"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT35527.1"
                     /translation="MNSLKASWVIGFAACVVAATASAQTTVPGYTGTNYDPPTPKWKG
                     QAGMTFLQIGGSARAEGMGGAFNGIKGDPSAIFYNPAGMASLGKAGVYANQTNWLAEM
                     KVTHFVGAGRVGPAIVGATFVNMDYGDIAGAQIVSSTVDPRGYQSTGNLSPETWSAGV
                     FGAIQMTDRFSTGVHVKYCVQDFSPSRIYSFSTRDYLATENRNRVGTFAVDLGTQYVT
                     GLRNIALNMSLQNFAQPQKYVEVKFDLPVTYRVGLSAELFELVTGNELSGQRVMLYAD
                     GMDQKDVSLDVAMGAEYLADLSQFVPGVKVGLRAGRRAAVNQDGWLSFGGSVQFGMSG
                     YNARFDYAYNDYGPGLTANRVGLSLTMD"
     gene            complement(9616..10389)
                     /gene="bshB1"
                     /locus_tag="PLT86_02565"
     CDS             complement(9616..10389)
                     /gene="bshB1"
                     /locus_tag="PLT86_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOT35528.1"
                     /translation="MSAYPDIPEEHLDALAIGPHRDDVELSCGATLVKLRKRGYRVGI
                     ADLTAGEMGTRGTAKERAEEARCAARALGITYRVNLGLPDARIEPDAHTMDALIRLIR
                     ATRPTLVLAPHPEEGRHPDHGAAGRLVPDAVFRAGLAKWETGQPHHRPLRVIHYMMHS
                     LFLPTFVVDVTEEWDAKMAAIRCYASQLNVPGANPGGEPTYISSPHFLGTIEARGRYY
                     GAQIAATYGEAFYQRELMRVEDPMSLALGSVERFAQKNA"
     gene            complement(10386..10853)
                     /locus_tag="PLT86_02570"
     CDS             complement(10386..10853)
                     /locus_tag="PLT86_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT35529.1"
                     /translation="MNPQDSFPPADRRCVIAIVDIAGFGDYVRTHANGDVFRLVDAFC
                     RHVECTVRRAGGEVVKMCGDAALVVFAETPVNQSVNALRMLRSRDGELHLATGESVRV
                     TIRAHYASVACGFIGTRGMRDVAGVGVNELFCLPAGEFVLSDALQHALNEDTP"
     gene            11035..11766
                     /locus_tag="PLT86_02575"
     CDS             11035..11766
                     /locus_tag="PLT86_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOT35530.1"
                     /translation="MSGATPQALDKAFQVVDAVTRLKGVTGALLIDPDGSTIAQDFRS
                     DDDLKRASAIGTTLPFELTKAAKSLKMGLVSDLTIQTVHGALRVCQNDGIWLLVFIGP
                     RANLGMLNIEMRAVRELFASVFQDDVKRGMDAEKQRILEVIESTGAVDSVIDKRRGDL
                     KAMRMLHEMVFRIAIDAGIGRETISRTINDINYRIYRDSLVDIGFDFFNRKALDTYDP
                     ALAERVMEEQIKGIAGLVTAQKGKG"
     gene            complement(11786..13120)
                     /locus_tag="PLT86_02580"
     CDS             complement(11786..13120)
                     /locus_tag="PLT86_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT35531.1"
                     /translation="MASKKGSGAAIRTGVFVILGFALFVGALFVLGARKQLFEDVYEI
                     YSTYEDVGGLQEGAFVRIAGINVGTVKKIALPELSQNRVVVTFSIRGDARRLIRRDSK
                     AIIDTEGLLGARIVIIVPGSPDEPEVPEGGTIQGQSPIQLHRFSANIDRALEHLDAVV
                     INSADLLAAGSSILQKVDAGQGTLGELVNNPALYDTVISSLAQFRRVAATADGTLRNV
                     QGLAGEIAGSVDRTLTHYSVAADTLRDAGSEFRRTAKATNTILRDLQEGRGTLGKLMT
                     DDSAYVALNNTIASSKQMLDRGTEAIAEITRAANSIARSAEQTRATIDLVSRNVMQGQ
                     GTVGRLLTDDSVYTRLDRVLANLDISSQKLAVNMEALRTNWLFRGYFEDAGYWENTKR
                     TMELVEQRNIRLNEWERRLQRIQADLEARERRLEAQSRQAAPARRDTSQESR"
     gene            complement(13127..13882)
                     /locus_tag="PLT86_02585"
     CDS             complement(13127..13882)
                     /locus_tag="PLT86_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT35532.1"
                     /translation="MIDLVNLHKSFGDLHVLRGVTFSVRKGETHVVLGRSGTGKSVSL
                     KCIVGLLDPDEGAVLAEGVEVAQMNQPTLEAWRSRVGFLFQGGALYDSMSVLENMVFP
                     LRSRKDLSEEDKTDLAHEKLRWVGLEGAVDKNPSDLSGGMRKRCALARTLMTEPEVIL
                     YDEPTTGLDPVTSNEISQLIARLKDERGVTGIVVTHDMPCARLVADSVTFLHRGSVLV
                     SGTWDTIRSCDKPLVQFFMASEFTREPLDIGEE"
     gene            14041..14682
                     /locus_tag="PLT86_02590"
     CDS             14041..14682
                     /locus_tag="PLT86_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT35533.1"
                     /translation="MAKRTTIKITPRVPRAPFSAPPSRVHHRARRPQKVGEPFEDGTP
                     EFQKPEYRFCPRCGTLLEPALEHDVVRPTCPECGFIVYYNPAPAVGAVITREGEILLV
                     KRAHPPKEGLWSLPAGFMEFGEKQLDALAREVREETGIEIRAATLLSVEDAADDPRTH
                     ALLIAFIADEWEGEPRPGDDASEVRWWPVGDLPQMAWQNHVRVVEKAREWLGL"
     gene            14667..15128
                     /gene="ruvX"
                     /locus_tag="PLT86_02595"
     CDS             14667..15128
                     /gene="ruvX"
                     /locus_tag="PLT86_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT35534.1"
                     /translation="MARPVRVLAVDYGDRRVGLAVSDELGIAAHGLPTLQVKSRKEAV
                     RAVVQAAKEHGVSRIVIGLPLNMDGTEGPRALVTREFGRNCEDALAAERCEIRVEMLD
                     ERLTTMRARAALREQGLRERSQRAKVDRVSATVLLQDYLAAQSATEGNDDG"
     gene            15118..15369
                     /locus_tag="PLT86_02600"
     CDS             15118..15369
                     /locus_tag="PLT86_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35535.1"
                     /translation="MTDDTRKGADCLSWPTIIHVAATDVGWLVILLPLSVVFRALLLI
                     ATGYYRLRRRSRFLGGAYALGVVGALAWLFARILRLWRD"
     gene            15369..16493
                     /gene="mltG"
                     /locus_tag="PLT86_02605"
     CDS             15369..16493
                     /gene="mltG"
                     /locus_tag="PLT86_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT35536.1"
                     /translation="MRNDPGKLSPQVSEGRERDSGYTDSLTVKALAGLAGVLALFAVV
                     VSAGWVALDTVRFLYTPPRGAGLYRVEIPAGATPREIGRILESHGIVRSARIFAYASW
                     RRGLTRAPRGGEYWLDGGQNVFRVLDQIARSGRPIRRVTIPEGYTVAEIAGVVERTLG
                     IPRDSMLALSRDTALVRRYALKGAPSLEGFLFPDTYFIDSGVTAEHVVRVLTQQFHRV
                     FTPEMVRRAEQLGMTPLEVITLASVIEKEAAVASERAVIAQVFHKRLKTGMGLESDPT
                     VKYAMGSGPVNLSLRDIQLDSPYNTYRYRGLPPGPICNPGKAALDAALYPADTDFLYF
                     VANWDGTHAFSKTLGEHNAAKAVSKRRYREWKAARGAAGT"
     gene            complement(16451..17836)
                     /locus_tag="PLT86_02610"
     CDS             complement(16451..17836)
                     /locus_tag="PLT86_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT35537.1"
                     /translation="MNEVLNWIQAVFAEYNLLFAKLLVHVAIVAMVASLLLRFRKFVA
                     LLLHEHPGTRERTEFGLVLGVIAAAGAWTRINLGYTSADLLVVAPLLSGFIMGTAAGV
                     WTGIIGGLVPLRHGELLAPVVGSIVGISAGITRASLKDPGSVWFFSPVPFSDTVATWR
                     RWRTERVLDPRLVVLLVGAGAEVFRTECARWTNPRWLFSYSPTEPVAYYIIVLMCLTS
                     VGVSLKIWNTPRMEARLRRQEALLAEARLDALRDQINPHFLFNTLSTINSLIRTHPDQ
                     ARIVIIKLSAILRRLLYSRENLCPLKAELDFVEDYLNIERIRFGADRLRLQKEIAPDV
                     VNARVPMMLLQPIVENAVKHGIGGREQGGTVTIRAHAEAGTLAIEVEDDGVGMTGRQL
                     EQSIRKGIGLSNIRERITSLYGDSGHLSLQSELGKGTLVRILLPLDTSTGFPEHPRQV
                     PAAPRAAFHSR"
     gene            complement(17833..18486)
                     /locus_tag="PLT86_02615"
     CDS             complement(17833..18486)
                     /locus_tag="PLT86_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT35538.1"
                     /translation="MIEHTTVAPVYLLCDMDGTLVDTEGLKDEALRRAIVDARGTDLD
                     PEEQTRIYSRYVGMPGIDIARAMIRHYCLAISADELWELREQHRRVLYSDAKALQARV
                     LSPVVALVERFREGIAVTGRGKTVLVTAAGGDQVHAVLEATKLGHLFDLVVSGREKSR
                     DNPACYRAALEACEALPEECLALEDSAVGYEAARSLDIPCLLLPNSYTRHQRLEKRP"
     gene            complement(18508..19611)
                     /locus_tag="PLT86_02620"
     CDS             complement(18508..19611)
                     /locus_tag="PLT86_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35539.1"
                     /translation="METESPLRVELPDPFMCDDGTRVTDPDQWPQRRREMLRTIVDTE
                     YGGLPPTPAGVRGERLSTNKMARILDAQYSQYRILLEDHPAFHIRLDLVVPPGEGPFP
                     VILNGDGCWRTLTDEILLAIMRRGFISAQFSRVEVVPDIALPERNSGLYAIYPDARFG
                     ALAAWAWGYHRCIDFLTTLDIVDTHAIAVTGHSRGGKAALLAGATDERVALTAPNGSG
                     CGGAGCYRWQGPKSETMAIIRGPFEFWFGPDLWQWIGREQEMPFDQHFLKAACAPRAM
                     LSTEGLGDLWANPEGTWQTYLAAREVFRFLGVEHNIGIHFREGGHQHGIADWTALLDF
                     AQWKFRGYPLPYRFDTSPFTHLPKAFSWSCPAT"
     gene            complement(19776..20414)
                     /locus_tag="PLT86_02625"
     CDS             complement(19776..20414)
                     /locus_tag="PLT86_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOT35540.1"
                     /translation="MKKNLWIFGSLAALSLAAVAVFAQTPSSPAADDEDGTPAMGMFA
                     MRGMGAGMGPGMCPPGTGMGMAAPGGQAMMCGRRGMGAGMAGRGMGLMRLQALNLTSD
                     QWARVRRIQEDFQKQIIPKQSEIRVAQIELQQLLQATQPNMNQVNAKLTAIGNLRTQI
                     QTLRTQEMIQIRGVLTQEQLTKFLDPSWQPPCAMQGMQAPAPGAGRRPMMNR"
     gene            20686..21618
                     /gene="trxB"
                     /locus_tag="PLT86_02630"
     CDS             20686..21618
                     /gene="trxB"
                     /locus_tag="PLT86_02630"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT35541.1"
                     /translation="MENHAKVLILGSGPAGLTAAIYAARADLAPLVVEGGQPGGQLII
                     TTEVENFPGFPEGIMGPELMDNMHRQAERFGARFVYGVATEVDLSRRPFVVDVEGTNY
                     TADTLIISTGASARLLGLESEARLMGHGVSACATCDGAFFRNQKVLVVGGGDSALEEA
                     NFLTKFASEVCIVHRRDELRGSQIMQKRVLANPKIRMIWNSVVEDILGDPATGVTAAV
                     LRNVKTNALTEEPCQGVFVAIGHSPNTEVFKGKLDLDEKGYIITKPHSTKTNVTGVFA
                     CGDVMDSSYRQAITAAGTGCMAALDAERFLAENE"
     gene            21896..>25923
                     /locus_tag="PLT86_02635"
     CDS             21896..>25923
                     /locus_tag="PLT86_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOT35542.1"
                     /translation="MRARIPGILSIALLGTVGQAAAAPAPAPPPVARPARAASDHVPL
                     AMRWLDINNFRCPITNYAFIGKNEIDNRAGGEWPAGSGEEYLFGSGILIAGTVNQEPF
                     TETLGARTAYSFRPSSTNPLGWQELGATESGAGSRAILTDPRAAIGYNPSGRVSEMSP
                     LTKVFVSAEPDWPLGVPTSVLDTYTEYDDQDPTRWNTGEGSNSGADTGEWSGTPDNTT
                     VATYGLGVKVKQTTYSWNYAGNSDIHFIVFDVENIRADGRAINNCFVGMVADPDIGSN
                     ASDDMTGFDATRNLGYAYDSDFSEVGFRRVPGFLGYRFLKSPVADHDIDRNGDGVIDG
                     NSVMINGVPVKDVHAGEQIGLHAFKSFNQANGDPDTEWEWYMAMAGHNFREDQAQIYQ
                     PFDFSNTPNDQRFLQSTGPFTLQPGQPVRIVVAMMVAKAAGNQGDPIEVRVAELLRTS
                     DVAQSIFDNNFVLPRPPVAPRLTARAGDGRIYLSWDTAAETTPDPFYAVASNPASTLY
                     DPRYRQYDFEGYRVWRSSTGNPNDWQLLADYDVVSAIPRDAVISLQAGTYSGTLIYDA
                     ATTEGYFLSRGGTDLFNGHEYLVMVEGGTQRVKVYDVSLGGQELPCNTSADGADWWFY
                     VGDNSRFELFDGNLNPLSSDPERNANGTYDQYVSGMVMYLGGMALRFADVPAPAGTAI
                     WRVVSSKEENFGGNTSVAHSYIDGGLRNGQLYHYAVTAYDFQLSSPSSLESAKSLSMI
                     SISPRHDPSDTTRVTTALHTSGAADCAVNVQVVDPMALTGHKYQIGFNPDGTWFVRDT
                     NLPAGQDIVINNQTNLSGDLNYPIVDGVLVNVIAPPVGIKSTSYSPSENKWFVERSAG
                     YEALTGQEWGWGTTVTAKDYARVEIRFNGGSTLAPVYLRGGTPNYAYVGAGTIPAEIW
                     DVTNNRRLAAAFVEQNGAVSRDGNWMPATAADGGREYLFVLAQPYNPDTLSADWDFFR
                     SKSILADASQFPLMTGSWIIAKEGMAPATGDVWAISPYLGNTPEDVFEYTTTHAPVAS
                     SLELPILAAGRREVVTFPVRGTLWNARSAQMMFLVGANTVQSVSIVHHAFQGQQGAQA
                     VAALVGDTVRVAMSGEEEVSLSNQALVVLSMTIRADADTGITHLAWLPAPLTSVDEEG
                     VNLVNGELYISFIRYGDVTADGSLTAQDATWILQSVVGIRTGVDVETADVSDNGSITA
                     YDAALVLFKVIHPEYVFPVRGGPLIKPSARAPVSLVWERTDAGWSLVVEDASGILAGD
                     VALALPDEVQVQVAADGAFAYRQDHRTLYVSFVHSAGDDRILFTVLTSAMETPALTAA
                     SLNEGAILLKFAQPAAFALLQNA"
BASE COUNT         4981 a         7527 c         8430 g         4985 t
ORIGIN      
        1 catgtgctct cgcattgcca cgcgtcgctg cacggatcgc ggcatgtggt gtcggttgcc
       61 ccgacagaca ttacggggtg gcgggagggt cccgtggtag cgcagtccat cagttacccg
      121 cgggtggtcc ggcttcccgg gggacggatg cttctctacc accgcgtgct ggggcacatg
      181 gggtactgga cgtaccagac ctccaacgac gggggattca cctggaaacg ccgcgcgacg
      241 ccgctggtgg atttcgacca gcgcccggag gcgccggggg acgagtgggc ggggagctac
      301 cacagcgtgg cgccgggaaa ggacggggag tcgctccaca tcgcgtttgt gtactgggac
      361 gagcggaagc gggtgcaccc ggtgtacggc atccggctgg cgcacatgga ccgctaccac
      421 ctgtactacg cacggctgga tatcgcctcc ggccggctgt attccgccgc gggaaagcgt
      481 cttggatgcc cggtgaaccg ccgggaggct gaaaaatgcc tggtgtggga taccggcacg
      541 cggctgacga acatgccgtc cattctggtg gaacgcgacg accggccggc gtttctgatg
      601 ccggtcaccg gcgaacgggt ggacgactgc gccttctggt gtattcgacg ggaacaaggc
      661 gcctggcagc gattccgggt gaccgacacg aacagcacct gggccggctc gcatctggag
      721 gcggacggag ccgggggcat cagcgcgttt ctcgtcggca ggcccggtcg gccggggcgt
      781 ctcccctacg gcggaggtac ccttcaggaa tggcgttccc cggaccgcgg cgaatcatgg
      841 gaacatatcg gggatattgt tccgaaggcg gggctggtct gcaacaaccc gaagccggtg
      901 gagacggccg ccggggaggt gctccggaga acgctggtgt tcttcgggtg ggaggggccg
      961 gacggccttg cgccggaggg accatatggc ggacaggcgt tcctgtggcg cgacggggag
     1021 tggctctgag gcgaatcggc gtctacgaac cgagtggagg aaacatgtgc cagatgttcc
     1081 ggttcgtctc gatacaccgt tctccgcttc gcgtcgcccg ccactcgacg accggcacaa
     1141 aaaaaacagc ggctcacatt cgcgtggaag ccgctgtttt tactgaaata cgcccggcgc
     1201 gattcgaacg cgcgacctgc ggattcgaag tccgacgctc tatccagctg agctacgggc
     1261 gcatgacgat accgtatttg tataatgtaa ggggcgggcc agttttgggt caattcacct
     1321 gtggatcgcg cacccggttg ccgcccgggc acttgaccgt cgcaggtctt ctgtgctaat
     1381 ggttgtacat gtggaggcac acgtccgggg agtgatggtt gtgcggcacc aatatcccga
     1441 gtaccaaaaa acgaaaatcg caaccacagg cttgacagtc tgaaatcagt gtgcgatact
     1501 agatgcagac acatgggatg ccagtgagtt tcgtgtagga gggaaatcac gaaagaacac
     1561 ccgtgtgaaa cggaactttc cacgagtata agtcaccatt tctctggtat ttgtgtggca
     1621 cacagggcgc gatcccgtgc aggggtggag tttttttctg cagggaacgg ggcccggttg
     1681 gatagtttct ggttctgatc acctgtttcc gaggagggga cgtgatgccc cgtagcatgt
     1741 cttcgtatca gcgatggttc gtagcggcag gaatcgcatt cctcagcgcc gtgtctctcc
     1801 ttcgcgcagg gacgaccggg aagattacgg gcattgtccg cgatcagagc ggtcagcctc
     1861 tgccgggtgt ggcggtgcag atcagcggcc tgcgactcgg cggcgtgacg gatgccgacg
     1921 ggcggtattt catcctccag gttccccccg gaacgcacga agtgcaggcc aacatggtcg
     1981 gctaccgcac ttcgatcacg cgcgacgtgc tggtgggtgc cgaccgcacc acgacgctca
     2041 acttcaccct tcgcgaggag acgattgagg tcgaggcggt ggtggttacg gcgggaagac
     2101 ccccgatcga ggttgacgta acgtcttccc agacaacggt aaacagcgcc cgcgtcgcag
     2161 aggttccggt caaccagatg ctggacgtgc tcgcgtacga accgggtgtt tctatcgcgc
     2221 gggataatga gttgaatatc cgtggcggtg gcgcgacgca gattcggttc caggtggatg
     2281 gacttgaccg caccgacgga ttgacgaaca agccgtacac ccagttgaac cagactctgg
     2341 tcgccgaggt gacgttgctg acgggtggtt tcaacgcgga gtatggaaac gttcggtcgg
     2401 gtatggtgaa cgtggtgacg aaggccggaa acgaacgcgg gcccaaccgc ggctgggttt
     2461 ccggtgtgta cagcatgacg ccgaccgccc gcaagaagca ttttggcccg ggcgggtaca
     2521 gcgaggatca gttcgactac cggacggctc tcctttcgga cgcgaatacg gtcggcgcga
     2581 cgggtgcggc ggacccctac ggcgcaatct actggccgaa cctgtatgaa tccacgcgga
     2641 cggataccgc gtttcgccgg gtctggacgg cggcgcccaa tacgtacaag gtgtttgacg
     2701 gatgggcaag ccgcgtgacc aacgcgaacg cgaccaaccg cggcgccggc gtgtacggca
     2761 aaggcacccg cacgaagccg tggacggctg accagatcat tgaagcgtgg aggtacgaag
     2821 cgaacctgaa tgagaacgtc tggggttaca gccacgagcc ggactggagc cttgatctgt
     2881 cggcagggtg ggggcttccc cgcaagcttg gcggtatcgt gataggatac gtcaccaaca
     2941 aggaaatgac gattaccccg gcgcttcggc cgtactatcg cgatgaaacc ttcgacgtga
     3001 agctcacgct tacgccggtg gacaggctga agtggagttt ctcgtacatg catggaacgg
     3061 gcgtttccac ggggaacggc acgagcagtt acgatccgga aatggcggaa accgcgggct
     3121 caatcgggat tggcggcgac gcagtgtcgc tccgcaccgc cggcgacctg cttggcacgg
     3181 tgtacatgtc gaaaggcagc tacggcaaca acaggctgaa tctttcctac aacagctttc
     3241 tggatgcgac attctggcag tggggtaccg cgctgacgta tacgatcagc ccggccacct
     3301 ttgccaccgc cgcgtttggt cagacccaca cggagtatga acttctccgg gactggccgc
     3361 gcgctgacat cagcgatttc agcacgaccg gcacgtacaa accctccagc acgttttcgt
     3421 atcgcgcgtg gttgcagatg ttcgcgctga gctggtcgga tgtcaacgga gacggcaggg
     3481 gcgatcttcc caacgatcta aatgacgcgt tgaccccggg ccgtgcgttc cttgagtatc
     3541 cgtacgggtt cccttccgcg tacaagtcgg ttccctctca gaccgtgtat gtgaaccgtc
     3601 agttcgtgtt tgcccccggc gacacggcgc gggtggtttc gccgcagggc tgggttcagg
     3661 aagggtatta tgatctgagc ggaaccttct ggcttggcga gggcggccag cagtatatga
     3721 cctcacacgc ggtgcagacg gcggcccgcg ctgacatcac tcatgcgatg ggcagccaca
     3781 cattgaagac gggcgcggag tatctgcttg ccgaccttga gtaccacgcg gaggaggcgt
     3841 cccggattat cggttcgcgg agcaactcca actaccgcga ttacggcggg aaattccccg
     3901 cgtcgcaacc gtcgattctc ggcatcttcc tgcaggacaa gttcgaatcg caggggatgg
     3961 tgatgaactt cggtctgcgg gcggagcggt tcgacggcgg ggcgccggtg ttcatgccgg
     4021 acatgattta cgacacgcgg ttgttcagcc cgaacaacgg gaagtactgg tttgacagcc
     4081 tctgcgtggc gaacgggtgg aacccgagcg acccggcatg gggggcgttg cccggttcca
     4141 tcggcgaggc ctggagcaag tttgagaaga acgggattct ggacaaattc ccgatgccgt
     4201 atgacgtggt ggcggccctg ccgcagcgcc cggcgaaggc ccattggcgt ctggcgccgc
     4261 ggtttgggat ctcccacccg gtgagcgtgc ggacgaagtt cttcttcaac tacggcatct
     4321 tctactccat ggccaagccg gcgcagatgt acgggctggg cgagcatgac gggcgcacgg
     4381 gtgccctcgg gcgttttgag cagttatact acccggagct ccggcccgca aagaccacca
     4441 tgtacgaagt agggttcgaa caggtgctcc cgttcagttt cgtgttcaaa acgacggggt
     4501 acgcgaagta caacgtggac caggtgacgg gcgtgcagat tcacgccggt gcgacggact
     4561 cgtaccgcac gtaccgcaac gcgaactacc aggatatccg cggtctggag atgaaacttg
     4621 cccgcaacgg ccggttcgta agcggatggg cgacctatca gatcatcgcc acgcgaagcg
     4681 ggcagacggg attgacggat ctctatcaga atccgacgat gaacgtgtac tacacgccgc
     4741 tggtgacgac gaattcgccg gcgaacaaca tcgcggcttc ggtgcgcgta ggcacaccgg
     4801 ttgaatgggg cctgttgacg ggcggctggg gcgccagtat tatcggacgg tacaacgaga
     4861 acaccggcga agtgatttac aaccctaata accaaccgcg gcgtgaactt ccgcccgagt
     4921 ggatcatgcg cgggcgggat tactggggcg ccgacctgaa gttcacgaag gacgtggcga
     4981 tcggccggca gcggcgcgca tcggtgtatc tggatgtgac gaatttcctg aaccgtaaat
     5041 atctgaacgg cacctacatg aatggcaacg attatcttgc ctacgtggtc gaacggagag
     5101 cacagggcga aaaggacctg cgtgtgggag acccctccac gtgggacgcg ttgacgcagc
     5161 cgtaccgcat cagaaaggcg gacggcacgt acacgcagtg gaaagcgcct atcagcccgc
     5221 gcacggactg gataatgttc ctgtatccca gagcgtaccg tgccggtatc aggtttgatc
     5281 tgtagcaccg ccaactgggt tgacgcatca gaaaggaggc caataatgac ggctactatg
     5341 cgtcgttggt tcacgtgggc cggtttgatg ggggcgttgg ttctgacgtt ctcgtcaacg
     5401 gtaatggcaa acacgccgca atcggtcatg catctcggcc gaatctggca ccatccggag
     5461 tacgacggag ccgagggctg gtcgggctcg ggcccgattt atcccgcggg tattccgacg
     5521 gctgccgacg gggacaactc cgtcaagcgc gggtggatag gtcaggtgca gaagttcggc
     5581 gtgtatctca ccacaacgga ctggacaagc ccgttgaaca ccgcgctgga gtatacatcg
     5641 tcctactgct tccgcagcat ggactatgcg tactaccgcg tgtacgtcac cgaaaagggt
     5701 gactacaact tcccggtggg gtgtcaggaa gtcttccgct ggacgcgtcc gacgattgtc
     5761 gcgggcgatt ccacgctcaa gttcttcccc ggacccggac cggcggcgta cccggataac
     5821 tccggcgtcg gccctcgtcc gactccggtg gtggatccga cccttgtgac ggaagaggcg
     5881 attgacgtga cctggcggtt cagccagggt gtcgagtatc gccgtcgtca gtacgggtac
     5941 ggcatcacca cggctcatca ggactacgtg ctgttcgaca cccggttcgt gaacaacggc
     6001 aagtgctatg gcgacacggt tcttgccgat ccggctccct tcacgatgac gggacagacc
     6061 atccacaaga tgatcttcgg gcaggcgtac gactaccgga acaaccacgc gccgggcgcg
     6121 aaacaggtcg gtaaggacaa cgaaggcatg tacatccagc cgtggggcgc ctcgttgaac
     6181 tcggtcgccc tgttctacga tctggacagc gacgatgccg ccgctcccgg acccgactgg
     6241 ggcgacccga gcgagaacgc cgcgatggaa ggccatctga cgggcaacgc gtacatggcg
     6301 atcggtcctg tgttcgcaag caagggcccc ggcagcctgt atggcacgaa cgaccccgat
     6361 cagccggcat tccggatgat gcacaacgag cgtggtctgg acttcgccgg caaggcgtat
     6421 tccccggcca actcgcagga attgcgtgag ttcctgaccg acggcgccct ccagatgccg
     6481 attgatcaga gcttccgcac gtatgctccg actgccgcga tcaaggacga ggatccgggt
     6541 gctaccgcca ttctgggcta cggcccgctg ggcggcgaca aggcgctgag ccccgccaat
     6601 gtgaaggttc atggttggga cctcgcgttt ggcgattcgg ttcgtctggt tcaggtggtt
     6661 gcgggtggtg gtattgatgc gtacgaaggt cagcgtatcg gccagtactg gaacgcgcag
     6721 aaggctgcca gcgccgctcc ggatacctgg atgagtgccg cggatcaggc tctcgttcag
     6781 accggtatgg acacggtgaa gaaggccctc gcgcttgcgt actggaactt ctacggctcc
     6841 ttcccggcca atgttgacgc cgcgaagaaa gccgcttggg gtattccgaa ctacgtgaat
     6901 tcgaagcctg ccgcgtacaa ccagccgttc aacgttcccg atcctccgcg tccgccggcc
     6961 ttcttcagtg tgaaggcgct ggcaaagggt ggtatcgtgg tgcgctggac tcgtgaatcg
     7021 gaatcggctc ccgacttcga taccggcgtg aacgacttcg ccggatatcg cgtgtatcgc
     7081 tgggaagcct cccgtctgac gaagcccgtc atcgtgaagc agggtccggc gagctccttc
     7141 tgggcagttg ctcaggatga agcgggtccc ggcccggctg gcttcgcgta tgtggacgac
     7201 caggttacca ccggtacgga ttactggtat gccgtgacgg cgtacgacga cggaacgcag
     7261 aactgggcgg aaccgggcaa gtcactggaa agcgcgtatt tctggtgctg gaccgggtac
     7321 acctcgaagg gcgtaacggc gccgacgttg aacggtcagg gtgttgatgt tgctcgcgcg
     7381 aatcggtttg cgctcgagca gaatgcgccg aacccgttca acccgtccac gacgatccgg
     7441 ttcgcggttc ccgcgaatgg caatgttcgt ctgacgatct acgacgtaaa cggccgcatc
     7501 gttcgcacgc tggttgatgg cagcgttctt gccggcagcc acgaagtggt gtgggatggt
     7561 atggacatga ccggtcgcgc ggttgcgagc ggcgtgtaca tctatcgcct cacgacggcg
     7621 aacaacgaga tgacgaagcg gatgacgctg gtgcgttagt ccggagttgg cggaacaccg
     7681 gtaccgggga cggcagagag ccgtccccgg tactgacaga taaacgattg aagcaacagg
     7741 cgaacgagca catcggagag cgggcagcgc gcaggcttct ccggtagacg tgtcgctttg
     7801 gccgggcgaa ggggtgccgt ggaggcgcag tgatggccag aggaccacgt atactatgcc
     7861 cgcgaagacg caccggaaag gacttggtga gatgaatacc cgattacgcc actggtggat
     7921 agccggagtc gcgtttgcat tcgtgctgat cgcattgccg cgagtttctc acagtgcggt
     7981 gagcacgctc gacagcgtta tcgttatccc gaatccgtat aacctgtcgg gacgcacgta
     8041 cggtccgcag cctcccggcg gttcaatcga ggcaaacgag cgtattcgtt tcgccaacct
     8101 gccggcgccc tgcacgatca aggtctacac gtccgcgggg aaccttgtgg cgacgatcaa
     8161 tcacacaggg gcgaatacgg ccgtttcgga tcttgagaac tactactgga gcgggcgcaa
     8221 cgcggacaac cagtacatcg tcagcgacgt gtatatctat gtggtggaat cacccacact
     8281 cgggcggaag attggtaagt tcatcgtgat tcgttagcct ggctgacccg tagtggttgc
     8341 cccgtagtgg ctgaccggtg ggtcagcacg ggtgacgctt cgtcagcagg ctggcggcac
     8401 agatagatga tgtgcagcgg actggctctc ggtgcgctga gaaggagctt ctggatgaac
     8461 agtttgaaag cgagttgggt gatcgggttt gccgcctgtg tggtggcagc gactgcgtcc
     8521 gcgcagacga ccgttcccgg gtataccggc acgaactatg acccgccgac gccgaagtgg
     8581 aagggtcagg ccggcatgac gttccttcag attggcggaa gcgcccgcgc tgaggggatg
     8641 ggaggcgcct tcaacgggat caaaggggat ccgtcggcga tcttctacaa tccggcaggg
     8701 atggcgtcgc tcgggaaggc cggtgtgtac gcgaatcaga cgaactggct ggccgagatg
     8761 aaggtgacgc atttcgtcgg cgcggggaga gtcgggccgg cgattgtggg cgccacgttt
     8821 gtcaacatgg attacggcga catcgcgggt gcgcagatcg tctccagcac ggttgacccg
     8881 cgcggatacc agagcacggg aaacctttcc ccggaaacgt ggtcggcggg tgtgtttgga
     8941 gccatccaga tgacggaccg gttcagcacc ggtgtgcacg tgaagtactg cgtgcaggac
     9001 ttcagcccca gcaggatcta ctcgttctcc acgagagatt accttgccac ggagaaccgg
     9061 aaccgcgtgg gaacgttcgc ggttgacctg ggcacgcagt acgtgacggg gctgcggaac
     9121 atcgcgctga acatgtcgct ccagaacttc gcccagccac agaagtacgt ggaagtcaag
     9181 ttcgacctcc cggttaccta tcgcgtcggc ctttccgcgg agctgtttga gcttgtgacg
     9241 ggcaacgagt tatccggcca gcgcgtgatg ctgtacgcgg atggtatgga ccagaaagac
     9301 gtgtcgctcg atgtggcgat gggagcggag tacctcgcgg atctttcgca gttcgtgccg
     9361 ggtgtgaagg tgggcctgcg tgccggacgt cgcgcggcgg tgaaccagga cggctggctg
     9421 agcttcggcg gcagtgtgca gttcgggatg tcgggctaca acgcgcgctt cgattacgcg
     9481 tacaacgatt atggccccgg acttaccgcg aaccgtgtcg gcctgagcct cacgatggac
     9541 tgattcacga tctgagtgca acgcgagcgg gcggcgggag caattccgcc gcccgctttt
     9601 tttgtggggg ggaggtcacg cgttcttttg cgcgaaacgt tccacggagc cgagggcgag
     9661 gctcatggga tcctccacgc gcatgagttc ccgctggtaa aacgcttccc cgtacgtggc
     9721 ggcgatctgc gcgccgtagt accgcccgcg cgcttctatt gtgccgagga agtgcggaga
     9781 ggagatgtat gtcggctccc cgccggggtt tgcgccgggg acgttcagtt gcgatgcata
     9841 gcatcgaatc gcggccatct tcgcgtccca ttcctcggtg acgtccacca caaatgtggg
     9901 cagaaagagg ctgtgcatca tgtagtggat gacgcgtaaa gggcggtgat gcggctgccc
     9961 ggtttcccac ttggcgagtc cggcgcggaa tacggcatcc ggcacgaggc ggccggcggc
    10021 tccgtgatcc gggtggcggc cttcctcagg gtgtggagcc agaacgaggg tgggacgggt
    10081 tgcgcggatg agcctgatga gtgcgtccat ggtgtgtgcg tccggttcga tacgggcatc
    10141 gggcagacca aggttgacgc ggtaggtgat accgagcgcc cgtgccgcac aacgggcctc
    10201 ttcagcgcgt tctttcgcgg ttccgcgggt gcccatctcg cccgcggtca ggtcggctat
    10261 tccgacccgg tatccgcgct tcctcagttt caccaacgtg gcgccgcagc tcagttccac
    10321 gtcatcgcgg tggggtccga ttgcgagggc atcgaggtgc tcttccggta tgtcggggta
    10381 cgcgctcacg gtgtgtcctc attcaaagcg tgttgaaggg cgtcagacag gacgaactcc
    10441 ccggcgggca gacagaacag ctcattcacg ccgacaccgg caacatcgcg cattccgcgg
    10501 gtgccgataa atccgcacgc cacgctcgcg taatgcgcgc ggatggtgac gcgcacggat
    10561 tccccggtgg ccagatggag ttccccatcg cgagatcgaa gcatgcgcaa cgcgttgacg
    10621 gactggttca ccggggtctc ggcaaatacg acaagggcag cgtcgccaca cattttgacg
    10681 acctcgccgc ccgcccggcg aacggtgcat tcgacgtgcc ggcagaatgc atcaaccagg
    10741 cggaacacgt cgccgttggc gtgtgttcga acgtaatcgc cgaagcccgc gatgtcaaca
    10801 atcgcgatga cacatcgccg gtcggcgggg ggaaacgaat cctgcgggtt catgccgggc
    10861 tcctgcttcc attcccgggt catggaacgg cataaggtgg cccgggcaac acgggcgcat
    10921 gcactctgtt gacgcgacag tcgtgtggaa cgatactcat aagtattgga cggatcgcgt
    10981 cgggcgcaat ccggccggat tgcgcgcggg ataattcctg caacgaggtt ttccatgagc
    11041 ggggcgactc ctcaggcgct ggacaaggcc tttcaggttg ttgatgccgt tacgcggttg
    11101 aagggcgtta cgggcgcgtt gctcatcgat cccgacgggt caaccattgc gcaggatttc
    11161 cgctcggacg acgacctcaa acgcgcttcc gccatcggca cgacgctgcc gttcgaactg
    11221 acgaaggccg caaaaagtct caaaatgggc ctcgtcagcg acctgaccat ccagaccgtt
    11281 cacggggcgt tgagggtttg ccagaacgac ggcatctggt tgctggtgtt catcggtccg
    11341 cgcgccaacc tcggcatgct gaacattgag atgcgtgccg tccgggagct gtttgcgtcc
    11401 gttttccagg acgatgtgaa aaggggaatg gacgcggaga agcagcgcat cctggaggtc
    11461 attgaatcca caggggccgt ggacagcgtc attgacaagc gacgcggcga cctgaaagcg
    11521 atgagaatgc ttcacgagat ggtgttccgc atcgcgattg acgccggcat cgggcgggag
    11581 accatcagcc ggacgatcaa cgacatcaac taccggattt accgcgattc tctcgtggat
    11641 atcggttttg atttcttcaa tcgcaaagcg ctggatacct acgaccccgc ccttgcggag
    11701 cgcgtcatgg aggagcagat caaggggatt gcgggtctgg tgacggcgca gaaggggaaa
    11761 ggctgagccc gcgatcagcc gtgcgtcagc gactttcctg gctcgtatcg cgccgtgcgg
    11821 gtgctgcctg ccgggactgg gcttccagcc gacgctcccg ggcttccaga tccgcctgaa
    11881 tgcgttgaag ccgccgctcc cattcgttga gccggatatt gcgctgctcc accagttcca
    11941 tcgtgcgctt cgtgttctcc cagtatcccg cgtcctcgaa atatccccgg aaaagccagt
    12001 ttgtgcgcag cgcttccata ttcaccgcga gtttctggga tgagatgtcg aggttggcga
    12061 gcaccctgtc cagcctggta tagacggagt cgtcggtcag gagcctcccg acggttccct
    12121 ggccctgcat gacgttgcgg gacacgaggt cgatggtggc gcgggtctgt tcggccgaac
    12181 gggctatgga attggcggcg cgggtgattt cggcaatggc ctcggttcca cggtcgagca
    12241 tctgtttgct gctcgcgatg gtgttgttga gggcaacata ggcggagtca tcggtcatca
    12301 gcttgccaag agtgcctcgg ccctcctgaa ggtcccgcaa gatggtgttg gttgcctttg
    12361 cggttcgccg gaattcggaa ccggcgtcac gcaaagtatc cgccgcaacg ctgtaatgcg
    12421 tgagtgtgcg gtcgacactg cctgcgatct ccccggcaag tccctggacg ttcctgagcg
    12481 ttccgtccgc ggttgcggcc acgcggcgaa actgcgccag ggaggagatc acggtatcgt
    12541 acagcgcggg gttgttgacc agctcgccaa gggtgccctg tccggcgtca accttctgga
    12601 ggattgacga accggccgcg agcaggtccg cgctgttgat gacaacggca tcaaggtgtt
    12661 ccagtgcgcg gtcgatgttt gcgctgaaac ggtgaagctg gatcggcgac tggccctgaa
    12721 tggttccgcc ttccggcact tccggttcgt caggcgatcc cggcacaata atcacgatac
    12781 gggcgccgag caggccttcg gtgtcgatga tggctttgga gtctctcctg atgagccggc
    12841 gggcgtcgcc gcggatactg aaggtgacga cgacacggtt ctgcgacagc tccgggagtg
    12901 cgatcttctt gaccgtgccg acattgattc ctgcaatgcg gacgaatgca ccctcctgca
    12961 ggccgccgac gtcttcgtag gtggagtaga tttcgtagac atcctcgaaa agctgcttgc
    13021 gggcgcccag aacgaacagc gcaccgacaa acagcgcgaa ccccaggatc acgaaaactc
    13081 ccgtgcggat cgccgcccct gagccttttt tcgacgccat ggttgcctat tcctcaccaa
    13141 tgtccagcgg ttcacgcgtg aactccgagg ccatgaagaa ctggaccagc ggtttgtcgc
    13201 aactcctgat agtatcccag gttcccgaca caagcacact tcctctgtgc aggaaggtga
    13261 cggaatccgc cacaaggcga gcgcacggca tgtcgtgggt aacgacgata ccggtaacgc
    13321 cgcgttcgtc tttcaggcgg gctatgagct ggctgatctc gttcgaggtg acgggatcca
    13381 gtccggtcgt cggttcatcg tagagtatga cttcaggctc cgtcataagc gtgcgagcga
    13441 gagcacagcg tttccgcatt ccgcccgaga ggtcggaagg gttcttgtcg accgctccct
    13501 ccagaccaac ccaacggagt ttctcgtgcg caaggtcggt cttatcttct tcactcaggt
    13561 ctttgcgtga gcgcagcggg aagaccatgt tttcgagcac gctcatggag tcgtacagcg
    13621 cgccgccctg gaagagaaag ccgacacggc tgcgccaggc ctccagcgtg ggctggttca
    13681 tctgcgcgac ctccactccc tcggccagta cggcgccttc gtccgggtcc agcaacccga
    13741 cgatgcattt cagggagacg cttttgccgg ttccggatct cccgaggacg acgtgcgttt
    13801 ctcctttgcg gaccgaaaag gttacgccgc gcagaacatg aaggtcgcca aacgatttgt
    13861 gcaggttcac gagatcaatc attcgtcgtt cccggccacg cgtgtggatg gggtgctcgc
    13921 tggcgtcgtt tgaggattaa gatacgcaaa tcggcgggct ccggcgaggg cagaagcaca
    13981 ccgcggagcg ggtgactccg cagcagggtt ccggtgcgac agggaaggag cagtcagagg
    14041 gtggcaaagc gaacgacaat caagattacc ccgcgcgtac cgcgcgcgcc cttttcggcg
    14101 ccgccatcgc gcgtgcatca ccgcgcacgg agaccgcaga aggtgggaga gccctttgaa
    14161 gatggtacgc cggagtttca gaaacccgag tatcgcttct gccctcgctg tggaacgctg
    14221 cttgagcctg ctcttgagca cgacgtcgtc cggccgacct gcccggagtg cggattcatc
    14281 gtgtactaca atcccgcacc ggcggttggc gccgttatta cgcgagaggg cgagatcctg
    14341 ctggtgaagc gcgcccaccc gccgaaggaa ggtctctgga gcctgcccgc cggtttcatg
    14401 gagtttggag agaagcaact ggatgcgctg gcccgggaag tgcgggagga gacgggaatc
    14461 gaaatccgcg cggcaacgtt gctttccgtg gaagacgctg cggacgaccc gcgcactcat
    14521 gcactcctga tcgcgttcat cgccgacgag tgggagggcg aaccgcgccc gggagacgac
    14581 gcctctgaag tgcggtggtg gcccgtcggg gatctcccgc agatggcgtg gcagaaccat
    14641 gttcgcgtgg tggagaaggc gcgggagtgg ctcggcctgt gagagttctg gcagtagatt
    14701 acggggatcg gcgtgtcggg ctggcagttt ccgacgaact cgggattgcc gcgcacggtc
    14761 ttcccaccct gcaggtgaaa tcgcggaaag aggcagtgcg agcggtcgtt caggcggcga
    14821 aggagcacgg cgtttcacgc atcgtgatcg gcctgccgct gaatatggac ggaacggagg
    14881 ggccgcgggc actggtgaca cgggagttcg gccggaactg tgaagatgca ctggcggcag
    14941 aacggtgcga gattcgggtg gaaatgctgg atgagcgcct gactacgatg cgtgcgcgcg
    15001 ctgctctgcg cgagcaggga ttgagagagc ggagccagcg cgcgaaagtg gacagagtat
    15061 cggccacggt gcttctgcag gattaccttg ccgcacaatc ggccacagag ggcaacgatg
    15121 acggatgata cccgcaaagg agcagattgc ctgagttggc ccaccatcat ccacgtggcg
    15181 gcgacggacg tgggatggtt ggtcatcctg ctgccgctca gcgttgtgtt ccgtgctctc
    15241 ctgctcatcg cgaccggata ctaccgcctt cgcaggcgga gccggtttct ggggggtgcg
    15301 tacgctctgg gtgttgtcgg ggcgctcgcc tggcttttcg cccgcatcct gcggctctgg
    15361 agagactgat gcggaacgat ccggggaaac tctccccgca ggtgtcagaa ggacgcgagc
    15421 gagacagcgg atacacggat tccctcaccg tgaaagcact cgcaggcctc gccggtgtgc
    15481 tggcattgtt cgcggtggtg gtgagtgcgg gatgggtggc tctcgatacg gtacgattcc
    15541 tgtacacgcc gccgcggggg gcaggtctct accgcgtgga gattcccgcc ggcgcgacac
    15601 cccgggagat cggacggata cttgaatccc acggaattgt ccgatccgcg aggatctttg
    15661 cgtacgcttc ctggcgcaga ggattaacca gggcgcctcg tggtggagag tactggctgg
    15721 acgggggcca gaatgtgttc cgcgtgcttg accagatagc gcgcagcggg cgtccgatcc
    15781 ggcgggtgac gatccctgag ggctacaccg ttgcggagat tgccggtgtt gtcgaacgca
    15841 cgcttggcat tccgcgggac agcatgctcg cgctttcgcg ggacacggca ctcgtgcgac
    15901 ggtatgcgct gaaaggcgcg ccatcgctcg aagggtttct cttccccgac acctacttta
    15961 tcgattccgg ggtgactgca gagcacgtcg tgcgtgtgct gacgcagcaa ttccaccggg
    16021 tcttcacgcc agagatggtc aggcgtgcgg agcaactcgg catgacgccg ctcgaggtta
    16081 tcacgctggc atccgttatt gagaaggagg cggcagttgc gtcggaacgg gcggttatcg
    16141 cgcaggtgtt ccacaagcgt ctcaagacag gcatggggct ggaatccgac ccgacggtga
    16201 agtacgcgat gggttcagga ccggtaaacc tgagtcttcg ggatatccag ctggattccc
    16261 cttacaatac gtaccggtat cgcggcctcc cgcccggacc gatctgcaac cccggaaaag
    16321 cggctctgga cgccgcgctg tatccggcag acaccgactt tctctatttt gttgccaact
    16381 gggatggaac gcacgcattt tcgaagacgc ttggagaaca caacgcggca aaggccgtga
    16441 gcaaacggcg ctaccgggaa tggaaagcag cacgcggtgc ggccggcacc tgacgcgggt
    16501 gttcagggaa gccggtggag gtatcgagtg gcaggaggat tctgacgaga gtccctttgc
    16561 caagttcgct ttgcagcgac agatgcccgc tgtcgccgta gagtgaggtg atcctctcgc
    16621 ggatgttgct caggccgatt cctttccgga ttgactgctc aagttgccgc cccgtcattc
    16681 cgacgccgtc atcttccacc tcgatggcaa gcgttcctgc ttccgcgtgt gcgcggattg
    16741 tgacagtgcc gccctgttcc cgccctccta tgccgtgttt caccgcgttt tccacgatgg
    16801 gctgcagcaa catcatcggt acgcgggcgt tgacgacgtc cggcgcgatc tctttctgga
    16861 gacgcagccg gtcggctccg aatctgatgc gctcgatgtt gaggtagtcc tcaacaaaat
    16921 cgagttccgc tttcaggggg cagaggtttt cccgtgagta gagcagacgc cgcagaatgg
    16981 cagagagctt gatgattacg atgcgggcct ggtcaggatg ggtgcgaatg agcgaattga
    17041 tggtgctcaa tgtgttgaac aggaaatggg gattgatctg gtcgcgcaac gcatccagcc
    17101 gcgcttctgc aaggagggcc tcctgccggc ggagccgcgc ttccatgcgc ggggtgttcc
    17161 agattttcag tgacactccg acggatgtga gacacatcag cacgattatg tagtaggcaa
    17221 ccggttcggt cgggctgtag ctgaacaacc atcgggggtt tgtccaccgc gcgcactcgg
    17281 ttcggaatac ctccgctccg gcgcctacga ggagaacgac aaggcgcggg tcgagcacgc
    17341 gttccgttct ccaccggcgc catgtggcaa ccgtatcgct gaacggcacg ggggagaaga
    17401 accagacgct gccggggtct ttcaacgatg cgcgcgtgat gccggcgctg ataccgacga
    17461 tgctgccgac gacgggggcc agcagctcgc cgtgccgcag gggaacgaga ccgccgatga
    17521 ttccggtcca cacccccgcc gcggtaccca tgatgaagcc ggaaagcagg ggagccacga
    17581 cgagcagatc cgcactggtg tacccgagat tgatccgcgt ccacgcacct gctgccgcga
    17641 tcacgccgag tacgagcccg aactccgttc gttccctggt gccggggtgt tcgtgcagga
    17701 gcagggcaac gaacttccgg aaacggagaa gcaggctcgc gaccatggcg acaattgcga
    17761 catggacgag aagcttggcg aagaggagat tgtattccgc gaaaacggcc tgaatccagt
    17821 tcagtacctc gttcacggtc ttttctccag acgctggtgc cgggtatagg agttaggaag
    17881 caggaggcag gggatgtcca gagaccgggc ggcctcgtac ccgacagcac tgtcttccag
    17941 agcaaggcac tcctccggaa gggcctcgca ggcttccagc gccgcgcggt aacacgccgg
    18001 gttgtccctg cttttttccc gtccagaaac caccagatcg aaaaggtggc cgagtttcgt
    18061 tgcctcaagg acagcgtgta cctgatctcc cccggcggcg gtgacgagaa ccgtcttgcc
    18121 tctgcccgtt acggcaattc cctcgcggaa ccgttctacc agcgccacaa ccggcgacag
    18181 tacgcgcgcc tgcagagcct ttgcgtcgga atacagaacc ctccggtgct gttcccgcaa
    18241 ttcccagagt tcgtccgccg aaatggcgag gcagtagtgg cggatcatcg ccctggcgat
    18301 gtcgattccc ggcataccga cataccggga gtaaatcctc gtctgttcct ccgggtccag
    18361 atcggttccc ctggcatcca cgattgcacg gcgaagagct tcgtctttga gcccctccgt
    18421 atccacaagg gtgccgtcca tatcgcacaa caggtacacc ggggcgaccg tcgtgtgctc
    18481 aatcaccgct cttcgctcct ttcggcgtca agtcgcgggg cagctccacg aaaacgcttt
    18541 cgggaggtgc gtgaacggac tggtgtcgaa ccggtacggg agcgggtacc cgcggaactt
    18601 ccattgcgcg aaatccagca acgcggtcca gtctgcgatt ccatgctgat gcccgccttc
    18661 acggaaatgg atgccgatgt tgtgctcgac tccgaggaag cggaacacct cccgtgccgc
    18721 gaggtatgtc tgccacgttc cttccgggtt tgcccagagg tcgccgagtc cttccgtgga
    18781 aagcatggcg cggggcgcgc acgcggcttt gaggaagtgc tggtcaaacg gcatttcctg
    18841 ttctctgcct atccactgcc acaggtcggg tccgaaccag aactcgaagg gtccgcggat
    18901 gatcgccatg gtttccgatt tcgggccctg ccagcggtaa catcctgcgc ctccacaccc
    18961 ggagccgttg ggggccgtca gagcaactcg ttcgtcggtt gcgcccgcaa gcagcgctgc
    19021 tttaccgccc ctggaatgcc cggtcaccgc gatggcatgc gtgtccacga tgtccagggt
    19081 ggtgaggaag tcgatgcacc ggtgataccc ccacgcccac gcggcaagag cgccgaaccg
    19141 cgcgtccggg tagatcgcgt acaacccgga atttcgttcc ggaagcgcaa tatccggcac
    19201 cacctctacc cggctgaact gcgctgagat gaatccgcgc cgcatgatgg cgaggaggat
    19261 ctcgtccgtc aacgtgcgcc agcacccgtc tccgttgagg atcaccggaa acggcccctc
    19321 gcccggcggg acgacgaggt caagacgaat gtgaaatgca gggtggtctt caagcaagat
    19381 gcggtactgg gagtactgcg catccaggat gcgagccatt ttgttggtgc tcaggcgttc
    19441 cccgcgcaca ccggcagggg tcggcggaag gcctccgtat tcggtatcaa caatcgtacg
    19501 cagcatttcc cgccgccgtt ggggccactg gtccgggtcg gttacccggg tgccgtcatc
    19561 acacatgaaa gggtcgggaa gctcaacgcg caagggggat tcggtttcca aagggatgtc
    19621 tcctgcgaga acagagtgga tcacggtttt caggttcggg aacgcgtacg aaatgtaaga
    19681 tgggagggga gcggaggaaa ccgaaacggc gtacacagag aggcgcctcc tgcgttccgg
    19741 caggaggcgc ctatgattct cgtatcggtg gtaaatcagc gattcatcat cggcctgcgg
    19801 ccagctccgg gagccggggc ctgcatcccc tgcatggcgc acggcggctg ccatgatgga
    19861 tcgaggaatt tggtgagttg ctcctgggtg aggacaccgc gtatctgaat catctcctga
    19921 gtacgcagcg tctgaatctg cgtgcgcagg ttgccaatgg cggtcagttt cgcgttgacc
    19981 tgattcatgt tgggctgggt ggcctgaagc aattgctgca gttcaatctg tgcaacacgg
    20041 atttcgctct gcttcgggat aatctgtttc tggaaatcct cctgaatcct ccgtacgcga
    20101 gcccactggt cggaggtcaa attcaacgcc tgcaggcgca tcagacccat accacgcccc
    20161 gccattcccg cgcccatacc gcggcggccg cacatcatcg cctgaccacc cggtgccgcc
    20221 atgcccatac cggttcccgg cgggcacatg ccgggcccca tacccgcacc cattcccctc
    20281 atggcgaaca tccccatcgc gggggtgccg tcttcgtcgt ccgcggccgg ggaagaaggc
    20341 gtctgcgcaa aaactgccac cgcggcgagg ctgagagccg cgagcgatcc gaaaatccac
    20401 aggttctttt tcacgttcca aacctccaga agagtagaag ctgtttaccg gcgccgtttc
    20461 acaaggatga acgggttggc ctggcgcttt attgtgttcc gcccactact cacggcggcg
    20521 gccggaggaa gacggcgcct gccgaactct gccggtgcgc cccctggttt cgtatactag
    20581 atgaacagac aaatggttcc gtcaagggca cgtttcgcgg tgcgggtttt tttgatcccc
    20641 gcgccagcac ggacgtcatc tcaaaggagt cccttcgtga gcaacatgga aaaccacgcg
    20701 aaagttctta tcctcgggtc cggtcccgcc gggcttaccg ccgctatcta cgctgcgcgc
    20761 gccgacctcg cgccgcttgt ggttgagggc gggcaaccgg gggggcagct catcatcacg
    20821 acggaagtgg aaaactttcc cgggttcccg gagggaatca tggggccgga gcttatggat
    20881 aacatgcacc gccaggccga gcgcttcggt gcgcgttttg tatacggtgt ggcaacggag
    20941 gtggatctct cacggcggcc tttcgtggtg gacgtcgagg gcacgaacta cacggcggat
    21001 acactgatta tcagcaccgg ggcttcggca cgcctgctcg ggttggaatc ggaggcgcgg
    21061 ctcatggggc acggcgtgtc cgcctgcgcc acgtgcgacg gtgcgttctt ccggaaccag
    21121 aaagtgctgg tggtgggtgg aggtgattcg gcgctggaag aggccaattt cctgacgaag
    21181 tttgcgtcgg aagtctgcat cgtccaccgg cgggatgaac tccggggctc gcagatcatg
    21241 cagaagcgcg tgctcgcgaa cccgaaaata cgcatgatct ggaactcggt ggtggaggac
    21301 atcctcgggg atccggccac cggagtgacc gcggcagtgc tgcgcaatgt gaagacgaat
    21361 gcgctcacag aagagccgtg ccagggagtg ttcgtggcga tagggcactc accgaacacc
    21421 gaggtattca agggaaagct cgatctggac gaaaaggggt acatcatcac aaagccgcac
    21481 tcgacgaaaa caaacgtcac gggtgtgttc gcgtgcggtg acgtgatgga ttcctcgtac
    21541 cggcaagcca tcaccgctgc gggaaccggc tgcatggccg cgcttgacgc cgagcggttc
    21601 ctcgcggaga acgagtgaac acacccggtt gcgtcctgtt ctgaaccacc cgtagccgcg
    21661 gttgcccccc tttcggggtt gggcacaccc ggccccgacc cggggcgaca acacctcgtg
    21721 actacccccg ccggctcgcg cctctgccga cggggggctt gtgtttcgcg ctgcgcggtg
    21781 tactattcgc tgtattccac ctttcccggc gcccttctct gtttgttcgc catagacagt
    21841 tcctgtcgcc cggcgagaca caatcccgcc ccccggcaga gagaggagac agacaatgcg
    21901 cgccaggatt ccgggtattc tctcgatcgc tctgctgggc acggtgggac aggcggcagc
    21961 agcgcctgcg ccggctccgc ctcctgtggc ccgcccggca agagccgcat cggaccacgt
    22021 gccgcttgca atgcggtggc tcgacatcaa caacttcaga tgccccatca ccaattacgc
    22081 gtttatcggg aagaacgaaa ttgacaaccg cgccggaggg gaatggccgg caggttcggg
    22141 cgaggagtat ctgttcggca gcggcatcct gatcgccgga acggttaacc aggagccctt
    22201 cacggaaacc ctcggcgcac gcacggcata ctcgttccgt ccctcctcga ccaatcctct
    22261 cggctggcag gaactcggcg caaccgaaag cggcgccgga agccgcgcaa ttctcacgga
    22321 tccccgtgct gcgatcgggt acaatccctc cggtcgcgtg agcgagatgt caccgttgac
    22381 gaaggtgttc gtcagcgcgg aacctgattg gccgctgggt gttcccactt cggtactcga
    22441 cacctacacc gaatatgatg atcaggaccc gacgcgctgg aatacgggtg agggatcgaa
    22501 ctcgggtgcc gacaccgggg aatggtcggg aactcctgac aacaccaccg ttgcgacgta
    22561 cggcctcggc gtgaaagtga aacagacgac gtactcctgg aattacgcgg gcaattctga
    22621 catccacttc atcgtgtttg acgtagagaa catccgggcg gacggaagag cgatcaacaa
    22681 ctgcttcgtt ggaatggttg ccgaccccga cattgggagc aacgcatcgg acgacatgac
    22741 ggggttcgac gccacgcgca acctcggcta cgcgtatgac agtgattttt ccgaggtcgg
    22801 gtttcgccgt gttccgggtt tcctcgggta ccgtttcctc aaaagccccg tggccgatca
    22861 cgatatcgac aggaacggcg acggtgtgat tgacggaaac tcagtcatga tcaacggagt
    22921 tccggtgaag gacgttcatg cgggagaaca gatcggattg cacgcgttca aatcgttcaa
    22981 ccaggccaac ggtgacccgg ataccgaatg ggaatggtac atggcgatgg cgggacacaa
    23041 tttccgcgag gatcaggctc agatttatca accgttcgat ttttcgaata cgccgaacga
    23101 ccagcgcttt ctgcagtcaa ccggcccgtt caccttgcaa ccgggccagc cggtgcggat
    23161 cgtggttgcg atgatggtgg cgaaagccgc gggcaatcag ggggatccca ttgaggtccg
    23221 tgttgcggag ttgttgcgta catccgacgt cgctcagtcc atcttcgaca ataacttcgt
    23281 gcttccccgt ccgccggtgg cgccgagatt gacagcgcgg gcgggggatg ggcggatcta
    23341 cctctcgtgg gataccgcgg cggagacgac ccccgatccg ttctatgcgg ttgcgtcaaa
    23401 tccggcttca acgctgtacg atccgcggta ccggcagtac gatttcgaag ggtaccgcgt
    23461 gtggagaagc tcgaccggaa acccgaacga ctggcagttg ctggccgact acgatgtcgt
    23521 gtcggcaata ccgcgtgatg cggtgataag cctgcaggct ggcacctaca gcggaacgct
    23581 catctacgat gccgctacca cggaaggcta tttcctcagt cgcggaggca cagatctctt
    23641 caacggccac gaataccttg tcatggtgga aggtggcacg caaagggtga aggtctacga
    23701 tgtcagcctc ggaggacagg aactaccgtg caacacttcc gcggacggcg cggactggtg
    23761 gttctacgtc ggggacaaca gcaggtttga gttgtttgac gggaatctca atcctctcag
    23821 cagcgacccg gaacgaaacg cgaacggcac gtacgaccag tacgtttccg gcatggtgat
    23881 gtatctgggc gggatggcgc tccgatttgc cgatgtgccg gctccagccg gaacggcgat
    23941 ctggcgtgtt gtttcgtcca aagaggagaa tttcggcggg aatacctccg ttgcccacag
    24001 ctacatagac ggaggtctca gaaacggtca actctaccat tacgcagtaa cggcgtatga
    24061 cttccagctt tcttctccga gcagccttga gagcgcgaag tccctgagca tgatatcaat
    24121 ttcgccgcgc cacgatccct cggataccac aagggttacc accgccctcc acacgagcgg
    24181 ggcggcggac tgcgccgtca acgtgcaggt ggtagacccg atggctctca ccgggcacaa
    24241 gtatcagata gggttcaacc ccgacggcac gtggtttgtg cgcgacacaa atctgcccgc
    24301 cggccaggac attgtcatca acaaccagac caatctttcc ggcgatctga attaccccat
    24361 tgttgacgga gttctcgtga acgtgatcgc tccgcccgtc ggcatcaaat ccacctcgta
    24421 ctccccgtcg gaaaacaaat ggttcgtcga acgctcggca ggctatgagg cgttgaccgg
    24481 ccaggaatgg gggtggggaa cgacagttac ggcaaaggac tacgcacgag tggagattcg
    24541 cttcaatgga gggtcaactc tcgcgccggt gtatctgcgc ggcggcacac cgaactacgc
    24601 gtatgtcgga gccggcacca tccctgccga gatctgggat gtgaccaata accgtcgcct
    24661 cgccgccgcg ttcgttgagc aaaatggcgc ggtctcccgc gacggaaact ggatgccagc
    24721 cacagcggcg gacggcgggc gcgaatacct gtttgttctg gcgcaaccgt acaacccgga
    24781 tactctgtcg gcggattggg actttttccg gagcaagagc attcttgccg atgcgtcgca
    24841 gttcccgctg atgacgggaa gctggatcat tgccaaagaa ggtatggcac cggcaacggg
    24901 cgacgtatgg gcgatttcgc cttacctcgg gaatacgccg gaggacgtgt ttgagtacac
    24961 cacgacgcat gcgccggtcg caagctcgct cgagcttccg atccttgccg cgggacgccg
    25021 tgaggtggtg acgttccccg ttagggggac gctatggaac gcgcgttcgg cgcagatgat
    25081 gtttcttgtg ggcgcgaata cggttcaaag cgtctcgatt gtgcaccatg cgttccaagg
    25141 gcaacaaggc gcgcaggccg tcgccgccct tgtcggtgac acggtgcgag tggccatgtc
    25201 aggggaggag gaggtttccc tgagcaatca ggctcttgtg gtgttgagca tgacgattcg
    25261 tgcggacgct gacaccggaa tcacgcacct tgcctggttg ccggccccgt tgaccagtgt
    25321 ggatgaggaa ggcgtgaatc ttgtgaacgg tgagctgtac atatcgttca tccggtacgg
    25381 cgacgtcact gcagacggct cgctcacggc gcaggatgca acatggatcc ttcagtcggt
    25441 cgtcggcatt cgaaccgggg tggatgtgga aaccgccgat gtgtcggaca atggttcaat
    25501 aaccgcgtac gacgcagctc tcgtgctctt caaggtaatt caccccgaat acgtgttccc
    25561 ggtgcgcggc gggccgctta tcaagccttc ggcgcgcgct ccggtttcgc tggtctggga
    25621 acggaccgac gcgggatggt cgcttgttgt ggaggatgcg agcgggatcc tggccggaga
    25681 cgtggcgctt gcgctgccgg acgaagtgca ggttcaggtt gcggctgacg gcgcattcgc
    25741 ataccggcag gatcacagga cgctgtacgt ttccttcgtt cattcggcgg gtgacgaccg
    25801 cattctgttc accgttctca cctctgccat ggagactccg gcgcttaccg ctgcgtcgct
    25861 gaacgaaggc gcaatactgt tgaagttcgc gcagccggcg gccttcgcgc tgttgcagaa
    25921 cgc
//
LOCUS       DAOSOO010000019        25806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1902_length_25806_cov_6.069318, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000019 DAOSOO010000000
VERSION     DAOSOO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_25806_cov_6.069318"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PLT86_02640"
     CDS             complement(<1..1377)
                     /locus_tag="PLT86_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOT35543.1"
                     /translation="MATSRHSIVSRQPAIRWQDGFPSGNGCIGALVYGSIAEERILFN
                     HEHLWLRIPKPELPDVSAHLPELRRLLEQGRYQDAQLFLSKRLEEAGYPHRRPAPYHP
                     AFDLALRMPTREAFRRYRRSLDFETGEVRVEWEDGGCAFQRDLFVSRADDVVVYRIRG
                     ERHFSGSVALVPHESTPSAPPSVTFHSSAEDGWLTFVGTHAEKDLYPEGNQFGGIARV
                     DISGGTASVTGNVITFTDAAEVLFRVGLFANEMAEAAIPEARSRIAALPESYEALKQR
                     HVELHRPLFLRTDITLDTAPDERSTEELLLDAYDGTVPVRLIETMFDFGRYLLISSSR
                     PGGLPANLQGIWNGEWNPPWQSDFHNDENIQMNYWEALPGSLPETLEPFFSYFESMVP
                     DYRENARKVYGCRGILLPICQTTDGMLYTDVWTNWTAGAGWIAQHFFDYWLFTGDKEF
                     LLRRAIPFM"
     gene            complement(1398..2813)
                     /locus_tag="PLT86_02645"
     CDS             complement(1398..2813)
                     /locus_tag="PLT86_02645"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOT35544.1"
                     /translation="MPAGLVMVWFRSDLRLNDNPALHFAASTGKSVLPVFVWNAPREI
                     PWAQGRASKWWLHESLVSLDASLRQKGARLVIRDGNPGMALATLAAETGADTVVWNST
                     REPDGRETDAELERQLRRAGISVKVYRSAFLFSPDNIRTREGKPFLVFTPFWNACRAE
                     KPPREPLPAPVRLPFPEVPVRSRSLDELGLIPAPDRTEGIRAVWKPGEPTAQKMLHEF
                     LVTRVDAYKTDREIPSIRGTSRLSAYLHFGEISPHTVWHHAFALNAEHFLRQLCWREF
                     ASHLLFHFPHAADHPLKTEYNRFPWNEDTAALRAWQTGATGFPFVDAGMRELWATGWQ
                     HNRVRMVTASFLTKDLLLNWTLGARWFWETLVDADLGNNTLGWQWVAGCGADAAPYYR
                     IFNPVLQGEKFDPKGEYVRRWIPELAGVSDRFIHRPWEAPAGELRAAGVRLGSTYPFP
                     IIHHEEARKRALSAYASLRRP"
     gene            complement(2817..4028)
                     /locus_tag="PLT86_02650"
     CDS             complement(2817..4028)
                     /locus_tag="PLT86_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT35545.1"
                     /translation="MCSASSRSTRPLAATAGYYGAFVAMGLMTAAVGPTLPGLAEQTG
                     STLRAISYLFSARSLGYLAGSFSAGHAYDRYPGHPILAGAVVFMALMALTVPFMPAIW
                     LLTIAMVGLGFGEGFLDVGGNAMLVWQHGARVGPFMNGLHFCFGLGAFVAPIVIAQVI
                     GITGGIRWAYWVLGGVAWLPAVWILSVPSPVIRKPADASVPGERNDRLLGLIVLFFFL
                     YVGAEAGFAGWIYTYALSLKLATATGAAYLTSVFWGSFTLGRLLAIPISAKVAPRTIL
                     VTDLAASICGVSAILIWQTSTIVVWAGTFVVGLSLASLFPTTVNLAEQRMVLNGRVTS
                     WFFIGASAGGMCVPWLIGQVFEQRGAVITMVMILCDLALTASVFLLMIRQIASATVRN
                     TSATTSVSPEV"
     gene            complement(4256..>5106)
                     /locus_tag="PLT86_02655"
     CDS             complement(4256..>5106)
                     /locus_tag="PLT86_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT35546.1"
                     /translation="VMETFGAKVHASPSNNTKAGREALAKDPNSNGSLGLAISEAVED
                     TVTSKDTKYSLGSVLNHVCLHQTVIGQEAKLQLAKAGLYPDVVIGCVGGGSNFSGLSF
                     PFVRDKINGKQVRAVACEPTSCPTLTRGLYKYDFGDSVGMTPLMLMYTLGHGFMPPGI
                     HAGGLRYHGDAPLLSQLVNEKLVEAVAVDQLPVFQAAELFARAEGIIPAPESAHALAG
                     AVVEADKARHEGKERVILIGLSGHGFLDLAAYDDYHQGKLKNFPLPQEEIDKALHELP
                     PIPANK"
     assembly_gap    5107..5206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5207..5775)
                     /locus_tag="PLT86_02660"
     CDS             complement(<5207..5775)
                     /locus_tag="PLT86_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT35547.1"
                     /translation="MDEVKIYLSEAEMPRHWYNIVADMPNKPAPPRHPATGEPVPPEA
                     FSAIFPMNIIEQEISAEREIAIPEEVLSFYRMYRPTPLIRAFRFEKALKTPAKIFYKY
                     EGASPAGSHKLNSAIAQVYYNKQAGTKRITTETGAGQWGSALAMACNFFGLECVVYMV
                     RVSYNQKPYRRIVMETFGAKVHASPSNNT"
     gene            complement(5842..6660)
                     /gene="trpC"
                     /locus_tag="PLT86_02665"
     CDS             complement(5842..6660)
                     /gene="trpC"
                     /locus_tag="PLT86_02665"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT35548.1"
                     /translation="MPEDRSMLERMVGAMIAEVECRKSRVPEREIIRKVHDMPAALSF
                     ESVIRRAGFAIIAEFKRASPSRGFIAREASPAQTAHSYQLAGATALSVLTNSEFFHGS
                     DDDLSAIRQTTTIPVLRKDFTVSSYQIYEARAIGADAILLIVAALDDYQLRDFLALAR
                     TVGLSAIVETHSEEEIERACGAGASIIGINNRDLKTFETTIQTTLSLAPLVPEDIAVI
                     SESGIHSGKDAALLRQAGVHGILVGESLMRAAQVSQDHLVRHMHELFGRSELRS"
     gene            complement(6653..7669)
                     /gene="trpD"
                     /locus_tag="PLT86_02670"
     CDS             complement(6653..7669)
                     /gene="trpD"
                     /locus_tag="PLT86_02670"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT35549.1"
                     /translation="MTVSEAIQRLASRTDLTRAETSDAMTQVMSGEATEVQIAGFLIG
                     LRTKGETVEEVAGAADAMRLKATRIPTRHGVLVDTCGTGGDRSGTFNISTTAAFVVAG
                     AGLPVAKHGNRSITSRCGSADLLSGLGVRIDLTPERVGACLDETGIGFLFAPVFHSAM
                     KYALEVRRQLAPTPTVFNMLGPLTNPAGASRQIIGVYRADMVELVARAAALLGTEHTF
                     VVHGSDGLDEITTTGSTKVAEVRNGATSFSEIKPEELGIPLATPGDIRGGDTSTNVEI
                     TRAVLAGAKGPHRDIVTLNAGAAIVAGGLAETLREGILRAEESIDTGRAQSVLRNLVD
                     FTNA"
     gene            complement(7666..8355)
                     /locus_tag="PLT86_02675"
     CDS             complement(7666..8355)
                     /locus_tag="PLT86_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT35550.1"
                     /translation="MPHQFDLVVFDLDGTLCDTAPDIGRCLNQAMEDFGLPPVPREKL
                     LKAIGPGGTAFHRAIVPNEEALPLAEKIVDRYRSYYTKSNTVLTRPFPGIIEMLDDLR
                     ATGLTMAVASNKPQEQTRQIVRGLGLMPYFAEVVGPEAVPRPKPEPDVLHFLIERVGR
                     GCPPGRTAMVGDTDNDMSAGRAAGVRTVFVTWGYMSESEIAPGSIDIVVSSPKELVAV
                     LTESRQKAEAK"
     gene            complement(8365..8934)
                     /locus_tag="PLT86_02680"
     CDS             complement(8365..8934)
                     /locus_tag="PLT86_02680"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOT35551.1"
                     /translation="MVLIIDNYDSFTYNLVQYLGELGAETVVLRNDSITVNDVESLHP
                     ERIVLSPGPCTPNEAGICLELVRALAGTLPVLGVCLGHEVIGQAFGGRIVRAPQLMHG
                     KTSEISHDGKGVFRKIPNPFTGTRYHSLMVDPTALPECLEISAETDGVIMGLRHRQYA
                     IEGVQFHPESILTTQGKAILANFLSLPNR"
     gene            complement(9014..10501)
                     /gene="trpE"
                     /locus_tag="PLT86_02685"
     CDS             complement(9014..10501)
                     /gene="trpE"
                     /locus_tag="PLT86_02685"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOT35552.1"
                     /translation="MSESEIAPSFEEAKRLVNAGAMLPVHRRILADTETPVSAYMKIR
                     AEDRYSFLLESVEGAEKFARYSFLGVDPFLKFSASGTRSEAHDRRSNTSDVRNGNPIS
                     HFRELLHQHRGIPLEGLPRFSGGAVGYFAYDAVRWVEKIPDSGLDDIGLDDAVFLFYD
                     TVLVFDSVAHQIILVSNVRLTDDESELRARYDAAVRKIAHLEKLLAYPVNPRPVRGTG
                     ISELSANMTREQFERAVEVAKEFIRAGDIIQVVLAQRFRVKIASGAFDIYRRLRIVNP
                     SPYMFFLALDGTEILGASPELLCRVESGVVHVRPIAGTRRRGATEDEDRHLEDELRAD
                     PKERSEHIMLVDLGRNDVGKVAIPGTVKVSEFMVVERYSHVMHLVSHINGRLREDQDR
                     LDAFFSCFPAGTLSGAPKVRAMEIIDELEPTRRGVYGGAIGYFDFAGNLDTCIAIRTV
                     LVKNGFAFIEAGAGIVADSDASSEYEETLNKSRAMISAIEMAEMR"
     gene            complement(10825..11046)
                     /locus_tag="PLT86_02690"
     CDS             complement(10825..11046)
                     /locus_tag="PLT86_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOT35553.1"
                     /translation="MSDVGKWIVVAGVILVIVGLAFMVSPKFPWLGRLPGDLVWKKGP
                     VTVYVPLATMLLISIVATALVNIIARWLR"
     gene            complement(11088..11468)
                     /locus_tag="PLT86_02695"
     CDS             complement(11088..11468)
                     /locus_tag="PLT86_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT35554.1"
                     /translation="MYSMEHVALAAKDTRTLANWYERVLGFKIVYESEKEPKAFFVQD
                     SKGMAIEIVPPLEDGNTVDANANHLAIWVDDFDRACADLRAKGVVLEPELTNPFFGKT
                     RIAFFNDCEGHRLQIIWRVRRIGE"
     gene            11655..15092
                     /locus_tag="PLT86_02700"
     CDS             11655..15092
                     /locus_tag="PLT86_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT35555.1"
                     /translation="MCDAWQRHSSDAQSTDALGGGNFIPSDEHQSGSKQLPDSAVFFA
                     LLESYPTGVLVVNQSGVIVAANAKAARFLKGVHLDVTGTSFLDFLSAESWQGFERTAK
                     TLVDAGQGQARLPVKGANGVIHVFEAVSKYPTTSDENDTVLILRDTTDYDMTLAANAE
                     DRLRILSRAVEQSQTVVLITDAEGTIEYTNEAFTAISGYSAEEARGKNPRILKSGHTT
                     EAEYRHLWDTIRSGETWYGTFYNRRKNGTFYWEAAKISPVRNNEGVITHFIALKEDIT
                     ARVEAEEELARRARYRMAIVEADRILLERESGEDLTPVLRVLGEASEAARVREFHNQR
                     DESGGLFASPVAEWVAPGVQLQIGNPALKYLPYEGTFSRWEKTLRSGRPIFGSVKDFP
                     PEEQRLLEAQHIAAVAVFPITKGTEWIGFIAFERCDEPPQPWTESEIALLGAAAASIS
                     LGVSRQAAQKALVESEERYRSVVNSVKEVIFQTDAEGNWTFLNPAWEGLTGFTVDQSI
                     GTSYLQYTHPEDKTENLELLASLSSGELESIRHETRYLTKNGEERWCEALVWVLRNDS
                     GAVRGAAGVIEDITEQKLAIEELRRAKEAAEAANRAKTEFLANISHEIRTPMNGILGM
                     TELALETQLSREQREYLQTVKTSADALLVLLNDMLDLSKIEAGQLELERIEFDFRATV
                     VTTVNTLLTRAEAKNITLDVRVDPNVPATVVGDPARLRQILLNLVGNAVKFTEVGGVS
                     VEVVAENGGKPGRTSIRALVRDTGIGIPVERQSAIFESFTQADATISRRFGGTGLGLA
                     ITKRLVNAMGGTINVTSTPGQGSTFEFVIPFEKGSARRERPAPGNQADSLLVRTVADT
                     QRDGTTRGDEKGSPNEPHGPEDGDTGSEDARRATALVVEDNATNLALARTILEKAGYR
                     VVAAGDGHQALAYLKAVRSPSDRPDVIFIDIQMPGMDGITATRSIRRMDTWKEVPIIA
                     MTAHAMKGDREKCLEAGMNDYLSKPVKKEELIEAARKWTRTGRTQRETSPRTRGGDPS
                     VDTEVFDAARLLDLVDGDETAFREITTTFRETTRDLVSRLKEANENKDVEAMRRVAHT
                     LKGSAGSFFAGQLQRASARLEQVCTDGTFEEIARNVATVVEAWNRLEDRMNAGNRAE"
     gene            15283..16341
                     /locus_tag="PLT86_02705"
     CDS             15283..16341
                     /locus_tag="PLT86_02705"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT35556.1"
                     /translation="MSAKQIVAVFMGGRASEHDISLKTGTVVMNSLDRTRYTVYPVQL
                     CRDGGWIIPAEPFSGIAPWSPFDEAAGAAPIEVALRQLRCIGPDVAFIALHGRFGEDG
                     CVQGLLELLDIPYTGSDVMSSALAMDKVRSKQIVAYHRIRTPRWIVVQEQEWRLQRSE
                     IIARVGREFGFPCVAKVPEEGSSFGMGIPQNETELEKVMDESIVARGHMMVEEYISGT
                     EITCAVLGGFPGEAPIALPLTEIIPKNSAYFDFEAKYTPGASEEITPARLDPEITALA
                     QDIGVRSHIALGCGGMSRTDMIVRDREVFYLETNTIPGMTETSLYPQAARAMGMDFRD
                     LLTRQVELAIAYHRRKRA"
     gene            complement(16359..17654)
                     /locus_tag="PLT86_02710"
     CDS             complement(16359..17654)
                     /locus_tag="PLT86_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT35557.1"
                     /translation="MSVPTSLAVLFVLLCLSAFFSASETALVAMTKVRVRRALDHYGR
                     KAKPLLQWDRHSDRLLTAIVIGNNVVNIGAASVTAMVLSYYIADPETAVWVNTALMTT
                     VILIFGEILPKQLGKSHSESTSLVVIRPVIALSSILWPLVWVFSGITRGVARWVMAGK
                     PRHSPVRSELEAAVAMAGEEGSLATDEHALLREALKLRETMVREIMTPRVQMVTLARH
                     DSLETAKSLCASSGFSRIPVTGADENEIVGVLFAKDLLLTYESGVGPTAVVVSDLMRP
                     PVFVPEVISVSRLLDAFRRHNTHLLIVVGEYGDVTGLVTMEDLLEKLVGAIEDEFDRE
                     EPEIKALSERRFLVAATAQPDSIARALGVVLPEGEYETVAGYLIDRLGKIPPPGTAHR
                     LEDWLILVYDADDRRVRSVTFQYLGMPPLSANKRPEVAL"
     gene            complement(17680..19167)
                     /locus_tag="PLT86_02715"
     CDS             complement(17680..19167)
                     /locus_tag="PLT86_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT35558.1"
                     /translation="MEPPTESRRHILVVAHRGAHQSCPENTVESIEQAIALGVDLIEV
                     DIRVTGDGHFVCLHNSTVDARTNGQGRVRDLTLTELKALDAGSKFSSAFAGASIPTVD
                     EVFARMRGHVGAYVDCKDAPPDSVADLLARHGLVEVSVVYADPAELREMIQREPRIRP
                     LPEYPGDVSAWHALADSIPTNHVAISSLANLSSESVRACHSRGAKVIVDIMAEDAPEG
                     WQRALEMGVDGLQTDRPAELLAFLRAKGLRRENVPPRVIEVIAHRGAHLNRPENTVPA
                     IEEAIAVGADWVEIDPAYTADRKLVILHDQKVNRTTDGTGKVTDLTLAEVRALNVKNP
                     DGSLAAGVRIPTLDEALAAMRGRVRIYLDAGGIDAHDLVEAMRRQGVSEQTAVYSDGR
                     TLAELRALEPAAFRLLPRPPEDLSTLPEIMERVQPAIFCASLRDITPAKVAACHRAGA
                     RVFCNVLSKDTPDGWGQAIECGADALETDKPAELLAFLRARSLHP"
     gene            complement(19278..20267)
                     /gene="nadA"
                     /locus_tag="PLT86_02720"
     CDS             complement(19278..20267)
                     /gene="nadA"
                     /locus_tag="PLT86_02720"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT35559.1"
                     /translation="MAVDTIVREDGVMLEPAPDPREMRKFADQNGILILAHSYQDGHI
                     QDAAHFVGDSLELARKAVEVKASRICFCGVHFMAETAKILNPESHVFMPDLEAGCSLA
                     EGCDAQDLARYQTHLKEKLGRDDLVTICYINCTAAVKALSDVVCTSGNALEVVNSVPR
                     GTPILFVPDVHLGGWVKENTDREIYLWNATCIVHELFSVDTLRALKKEHPDAQILAHP
                     ECPKNIRDEADFVRGTAGMLRIVTANRGKTYIVATEGNMVHRFKTEAPENTYVAAPGP
                     SCACNLCPHMQKNTPEKLWYSLRTGRYEVFVDDAVASNARKALERMLAVRVTR"
     gene            complement(20476..21543)
                     /locus_tag="PLT86_02725"
     CDS             complement(20476..21543)
                     /locus_tag="PLT86_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35560.1"
                     /translation="MPRETMTPRERWEAVLRRQKPDRIPMDYWATPEATRKLQAHLGV
                     NSTEELHAKLHIDATLSVGGNYVGPPAPEGEDVFGIRYRTVEYATGVYTEAVHNPLAR
                     YRSVEEIEANYRWPQADWWDYSHIPDQIRGNEHRPIAGGGSEPFLRYCQLRGLEQAFV
                     DLVENPEIVHYCLDKLFDLAYTNTLRIYEQAARSTASILSSYVAEDMGSQESLLFSPA
                     QIHTFLLPRMKRMIDLAHSAGVFVFHHSDGAVRPIIPDMIEAGIDVLNPVQWRCNGME
                     REALKRDFGDRLVFHGGVDNQQTLPFGSVDDVRNEVKENIRILGAGGGYIIAPCHNIQ
                     AVSPAGNVVAMYETGYHEGWT"
     gene            21702..22283
                     /locus_tag="PLT86_02730"
     CDS             21702..22283
                     /locus_tag="PLT86_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35561.1"
                     /translation="MVDIPVGMYRPHMRDIPLFALPDGFVFRDFRAPGDDGSGDAAAW
                     VRIQAAAEAQFMQITHETFLNDFGGHESQLPGRSWFVTTEKGEDVASITAWWWDTPPD
                     CELQGRVGLIHWVATIPEYQGRKIGKAMMTKAMQRLARDHQVAYLNTSTIRIPAIRMY
                     LDFGFLPDLKKNRADEAWSLVRESLKHSALGNA"
     gene            complement(22292..22933)
                     /locus_tag="PLT86_02735"
     CDS             complement(22292..22933)
                     /locus_tag="PLT86_02735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT35562.1"
                     /translation="MIESGRIFDVTRTLGFDTPPYPGDPAFEREAKECGGVTISRLTM
                     SSHSGTHLDAPAHVFPGGKTLDEYPPERFFLSAQVVEAKSERVITAADVNALRLEEYE
                     AVLFKTLFIGPPDAHKPLSWISLSPDAAAALVRRGVRLVGIDSPSVDAADATELPVHR
                     TLLGSDVLVLENCDLREVPAGGYELVCMPLRVRTSDGAPCRCVLIMNGERATP"
     gene            complement(22930..24744)
                     /gene="pabB"
                     /locus_tag="PLT86_02740"
     CDS             complement(22930..24744)
                     /gene="pabB"
                     /locus_tag="PLT86_02740"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOT35563.1"
                     /translation="MTENRTGTGHAPRVILRDARHGQWLKFVDPQRTLVATTLQEVLQ
                     VLETIGRAAAEDALYCAGFVSYEAAPAFDAGLAAHTPGRFPLAWFCLFRNVEPVDIPA
                     PPANALSRAFRWRPSVSRTEYYEAIHRVHALIESGDTYQVNYTYRLVSEFTSDPWEFF
                     LRLVAAQNPPYGGYIDAGEWAICSASPELFFTRHGSELESRPMKGTAARGRTLEEDLL
                     MSERLQASLKERAENVMIVDMVRNDFGRVAQTGSVAVKRLFEIEQYPTIWQMTSTVTA
                     RTEQRLQNVFRATFPPASITGAPKIRTTQIIADLETTRRNIYTGTIGFVTPGGDAQFN
                     VAIRTALIDRTTGKAEYGAGSGIVWDSDAESEWEECETKAKVLSAEVPEFQLLETMRW
                     EPRSGYYLKTHHLRRLTESARYFGFKVDMREVRRELRSFARTLPRKAQRIRLLVEKSG
                     AISLEAFPLDRRIGNNLRVAVARKPIHTSDVFVFHKTTNRRVYESAMKENPRCRDVIL
                     WNEKGEVTESTVANVFVNIGGRLFTPPVACGLLGGTMRAHLINTGRVTERVISISELL
                     RSPVVYLGNSVRGMYRVRVMRTTAHDLEAPDARARRHA"
     gene            complement(24737..25234)
                     /gene="rnhA"
                     /locus_tag="PLT86_02745"
     CDS             complement(24737..25234)
                     /gene="rnhA"
                     /locus_tag="PLT86_02745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOT35564.1"
                     /translation="MKEIRIHTDGACSGNPGPGGWAAILTYGTHRKSLFGAEPETTNN
                     RMELRAAIEALRALKEPCNVVVVTDSRYLRDGITKWLPNWKKNGWKTSAKDPVKNRDL
                     WEALDCLTGVHSVRWEWVEGHSGDPDNEACDVLAVTAMKKLLKRAPAARTRPQSVAGA
                     THRDD"
     gene            25320..25778
                     /locus_tag="PLT86_02750"
     CDS             25320..25778
                     /locus_tag="PLT86_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35565.1"
                     /translation="MATGRDGPTMIEDLNPAFDMFLFPAKVSARVRRYFSLFDRALAN
                     LLAHFDVEAGLIRHAVPEGSPGVSEEVADLDRFERKKYISPLNKWAYGVGRQSAAAIG
                     AFGYGYAQRMSMYSGDSHILRAVRNGLEAFARIQVPSGEFVFTPIRFCSV"
BASE COUNT         5337 a         7485 c         7492 g         5392 t
ORIGIN      
        1 catgaacgga atggcgcgcc gcaggaggaa ctccttgtcc cctgtgaata gccagtagtc
       61 aaagaaatgc tgcgctatcc agcccgcccc cgcagtccag ttggtccaca catcagtgta
      121 gagcatcccg tcggtcgtct ggcagattgg caaaaggatt ccgcggcacc cgtagacttt
      181 ccgcgcgttc tcccggtagt ccggtaccat ggactcgaaa taggaaaaga aaggttccag
      241 tgtctcgggc aggctcccgg gcaacgcctc ccagtagttc atctggatgt tctcatcgtt
      301 gtgaaaatcg gattgccagg gaggattcca ttcaccgttc cagatgccct gcagattcgc
      361 cggcaggcct ccgggtcgag agctgctgat gagcaggtac ctgccgaaat cgaacatggt
      421 ttcgatgagg cgcactggta cggtgccgtc gtaggcatcc aggagcagtt cctcggtgga
      481 gcgctcatcc ggcgcggtgt caagcgtgat gtcggtccgg agaaacaggg gacgatggag
      541 ctccacgtga cgttgtttga gggcttcata cgattcagga agcgccgcaa ttcgcgacct
      601 ggcttccggg attgctgctt ccgccatttc gttggcaaac agcccaaccc ggaagagcac
      661 ttctgcggcg tctgtgaacg tgatgacgtt ccctgtgacc gaggcggttc cgccagaaat
      721 gtcgacccgg gctattccgc cgaactgatt cccttcgggg tacaggtcct tttccgcgtg
      781 ggtccccaca aacgtcaacc atccgtcctc ggcggaggaa tggaatgtca ccgacggcgg
      841 cgcagagggt gtgctctcgt gaggcacgag cgcaacgctg cccgaaaagt gtctctcacc
      901 gcgaatcctg tatactacca catcgtccgc gcgcgacacg aacaggtccc gttggaaggc
      961 acagccgcca tcttcccatt ccacccggac ttcgccggtt tcaaaatcga gggaccggcg
     1021 gtatcgtcgg aacgcctcac gagtcggcat tcggagcgcg agatcgaagg ccggatggta
     1081 cggtgccggg cgccggtgcg ggtaccccgc ctcctcgagt cgcttgctga gaaaaagctg
     1141 tgcatcctgg tatcgtccct gttccagcag tcttcgcaat tcgggaagat gggctgaaac
     1201 gtcgggaagc tccggttttg gaatccgcaa ccagagatgt tcgtgattga aaaggattcg
     1261 ttcttccgcg atcgatccgt agacgagggc cccgatacag ccgtttcccg aagggaaccc
     1321 atcctgccaa cgaattgccg gttgccgaga gacgatcgaa tgcctggatg ttgccatgga
     1381 aatccctttc cgacaatcta cgggcgcctg agcgacgcgt acgccgaaag agcccgcttg
     1441 cgtgcttctt cgtggtgaat gatggggaag ggatacgtgg aaccgagtct cacgcctgcg
     1501 gcccggagtt cacccgccgg tgcctcccat ggcctgtgga tgaacctgtc ggatactccc
     1561 gcaagttctg gtatccagcg gcggacgtac tcgcccttcg gatcgaattt ctcgccctgc
     1621 agtaccgggt tgaagattcg gtaatatggg gcagcatccg caccgcaccc ggccacccat
     1681 tgccagccaa gcgtgttgtt cccgagatca gcatccacca gggtttccca gaaccagcga
     1741 gcgccgagcg tccagttcag gagaaggtct ttggtgagaa acgatgcagt aaccatgcgc
     1801 acgcgattgt gctgccatcc tgttgcccaa agctctctca tccccgcatc gacaaacgga
     1861 aatccggttg cgccggtttg ccaggcgcgc agggctgcag tgtcttcgtt ccaggggaac
     1921 cggttgtatt ctgttttcag gggatgatcc gcggcgtgcg ggaaatgaaa gagcaggtga
     1981 gacgcgaatt cccgccagca gagctgacgg aggaagtgct ccgcgttgag cgcaaatgcg
     2041 tggtgccata ccgtgtgcgg gctgatttcg ccgaaatgga ggtacgccga aaggcgagac
     2101 gtcccgcgaa tgcttgggat ttccctgtcc gtcttgtacg catctacgcg cgtaaccagg
     2161 aactcgtgga gcattttctg cgcagttggt tcacccggct tccatacggc acgaattcct
     2221 tccgtgcgat ccggcgcggg aatcaggcca agttcgtcga gcgaacgtga acggaccggt
     2281 acttcgggaa acggtagccg cacaggcgcg ggcagaggtt ctcgtggtgg tttctccgca
     2341 cggcacgcgt tccagaacgg agtgaatacg aggaatggtt tgccctctcg tgtccggata
     2401 ttgtcgggcg aaaaaagaaa cgccgaccgg tagactttga ccgaaattcc cgcgcgccga
     2461 agctggcgct ccaattcggc gtcggtttcc cgtccatccg gctcccttgt ggaattccat
     2521 acgaccgtgt ctgcccctgt ttccgccgca agggtggcca gagccatacc cggattccca
     2581 tcgcgaatga ccagccgcgc tcccttctgc cggagcgacg cgtcaagtga gaccagggat
     2641 tcgtgtaacc accacttgct ggcccttccc tgcgcccagg ggatttctcg tggcgcgttc
     2701 cacacgaaaa ccggcaaaac cgattttccg gtggatgcgg cgaagtggag ggccgggttg
     2761 tcgttgaggc gcaaatcaga ccggaaccat accatcacaa ggccagcagg cacagctcac
     2821 acctccggac tcacgctcgt tgtggccgac gtgtttctga cggtggctga agcgatctgg
     2881 cggatcatca acaggaatac actcgctgtc agggccagat cacacagaat catcaccatc
     2941 gtgataacgg cgcccctctg ttcgaaaacc tgcccgatca accatggaac gcacatccct
     3001 cccgcgcttg ccccgatgaa aaaccagctg gttacccgac cattcagaac cattcgctgc
     3061 tccgcaagat tcaccgtagt cgggaacagg gaagctaacg agagcccgac cacgaacgta
     3121 cctgcccaca ccacgatggt tgatgtctgc cagattagta tggcgctgac cccgcaaatc
     3181 gaggctgcga ggtcggttac gaggatcgtg cgcggagcga cctttgcgct gattgggatt
     3241 gccagaagtc ggccgagggt gaacgagccc cagaacaccg acgtaaggta tgccgcaccc
     3301 gtggcagtgg caagtttcag ggaaagcgcg tacgtgtaga tccagcccgc gaaaccggct
     3361 tcggctccga cgtacagaaa gaagaaaagg acgatcaggc cgagaagccg atcgttcctt
     3421 tctcctggta cgctcgcgtc cgcgggcttg cgaatcacgg ggctcgggac gctgagaatc
     3481 cacactgctg gaagccacgc tactccgccc aatacccagt atgcccagcg gatgcctccg
     3541 gtgataccga tcacctgcgc gatcacgatc ggggcgacga atgcgccgag cccgaaacag
     3601 aagtgcaggc cattcatgaa cggtccgacc ctggccccgt gttgccacac gagcatcgca
     3661 tttccgccga cgtccaggaa accctcaccg aatccaaggc caaccatagc gatggtgaga
     3721 agccagattg caggcatgaa aggaacagtg agggccatca atgccatgaa tacgacggca
     3781 ccggccagaa tcgggtgccc ggggtaccga tcgtacgcat gcccggcgga gaacgaaccg
     3841 gcgagatacc ccagcgatcg cgccgagaag aggtaactga ttgcgcggag ggtgctccca
     3901 gtctgttcgg cgagcccggg tagtgtcggc ccgactgccg cagtcatcaa ccccattgcg
     3961 acgaacgccc cgtaataccc tgccgttgca gccagcggac gggttgatcg ggacgaagcc
     4021 gagcacatgg gaacgcctgc agaaggaagg aggtgacacg aaaacggagg agaaaagaaa
     4081 acatcttcag cccggcaatc caaccggcgg cacccgggag cgcttcaggt gccgcgaaag
     4141 acggtcagca aaaaggcatt cacgatcggg gaaccgcagg gagttcccgc aaaaagcaaa
     4201 cgaggacggc tcgtaagacc gtcctcgctg gcttctctgc ttgctggctt acacgtcatt
     4261 tgttcgcggg aatcggaggc agctcgtgaa gcgccttgtc gatttcctcc tgtgggagtg
     4321 ggaagttctt cagcttgccc tgatggtaat cgtcatacgc agcgaggtcg aggaacccgt
     4381 ggccgctgag acctatcagg ataacacgct ccttaccttc atggcgtgcc ttatctgcct
     4441 ctaccaccgc accagcaagg gcatgcgccg attctggagc cggtataatc ccctccgccc
     4501 gagcgaacaa ctcggccgcc tggaaaaccg gcaactgatc cacagccact gcttccacga
     4561 gtttttcgtt gacaagctgg ctgagaagtg gggcgtcgcc gtggtaccgg agtccgccgg
     4621 cgtgaattcc cgggggcatg aatccatggc ccagggtgta catgagcatc aagggtgtca
     4681 tgccgacgct gtcgccgaag tcgtacttgt aaaggcccct tgtcagcgtc gggcaactcg
     4741 tcggttcgca cgccacagcc cgaacctgct tcccgttgat tttgtcgcgc acgaatggga
     4801 atgaaagccc ggagaagttg ctgccaccgc cgacgcaacc gattactacg tcagggtaca
     4861 gaccggcttt cgccagttgc agcttcgctt cctggccgat gaccgtctgg tggaggcata
     4921 cgtggttcaa cacgctcccc aggctgtatt tggtgtcctt gctggtgacg gtgtcttcca
     4981 ctgcctcgct gatggccaga ccgaggcttc cgttcgaatt cgggtctttg gcaagggctt
     5041 cccgacccgc cttcgtgttg ttggatgggc tcgcatgcac tttcgcgccg aaggtttcca
     5101 taacgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcg tgttgttgga
     5221 tgggctcgca tgcactttcg cgccgaaggt ttccataacg attctgcggt aaggtttctg
     5281 attgtagctt acgcgaacca tgtacacgac gcattccaga ccaaagaaat tgcacgccat
     5341 ggccagggcg ctgccccact gcccggctcc tgtttccgtg gttatgcgtt ttgttcccgc
     5401 ctgtttgttg taatacacct gtgcgatggc ggaattcagc ttgtggctgc ctgccgggct
     5461 cgcgccctca tatttgtaga aaatcttcgc aggcgtcttg agcgcttttt cgaaccggaa
     5521 tgcccggatc agaggggttg ggcgatacat cctgtaaaag gacaagacct cctcagggat
     5581 ggcgatctcc cgttcagcgc tgatttcctg ctcgatgatg ttcatcggga atatggcgct
     5641 gaaagcctcc gggggaaccg gttcgcccgt cgcagggtgg cgaggtggcg ccggcttgtt
     5701 tggcatgtcg gcgacgatgt tataccagtg ccgcggcatc tcggcctcgc tcaggtagat
     5761 tttcacttcg tccattctgt gttcctccac ttctttgtgt ggtgtggcgg cgccgcaaag
     5821 gcccccgcgc ggttgatggt cttatgatcg caattcgctt ctaccgaaca actcatgcat
     5881 gtgacggaca aggtgatcct gtgaaacctg ggctgcgcgc atgagtgact ctccaacgag
     5941 gataccgtgt acacctgcct gcctgagcag ggcggcgtcc ttgccggagt gaatcccact
     6001 ttcactgatc acggcgatgt cttcgggtac cagcggcgcg agcgaaaggg tcgtttggat
     6061 ggtagtttcg aaagttttga ggtctcggtt gttgatgccg atgatgctgg cccctgcgcc
     6121 gcacgctcgt tcgatttctt cttcggaatg ggtttccacg atcgcggaga gcccgacggt
     6181 tcgcgccagg gcaagaaagt ctctcaactg gtaatcatcg agtgctgcta cgatgagcag
     6241 tattgcgtcg gccccgatag cccgggcttc gtagatctgg tacgagctca cggtgaagtc
     6301 tttccgaagt accgggatgg tggttgtctg cctgatggcg gaaaggtcgt catctgaccc
     6361 gtggaaaaac tccgaattgg tcaaaacgct gagagctgtt gcgcctgcca gttgatacga
     6421 gtgcgcggtt tgtgcgggcg atgcctcccg ggcgatgaac cctcgtgacg gcgaagcccg
     6481 tttgaactcg gcgatgatcg caaaccccgc gcgcctgatg accgactcaa aggaaagggc
     6541 agcaggcatg tcgtgaactt tccggattat ctcacgttcc ggaactcgag acttgcggca
     6601 ttcgacttca gctatcatcg ctcccaccat ccgttccagc attgatcgat cttcaggcat
     6661 ttgtgaagtc cacaaggttt cgcagaacag actgcgcgcg cccggtgtcg attgattcct
     6721 ctgcacgcag gatgccttcc ctcagggttt ccgccagtcc tcctgcaaca atcgccgcac
     6781 ccgcgttcag ggtaacgata tcccggtgcg gcccttttgc tcctgccaaa acggcgcgag
     6841 tgatttcaac gttcgttgag gtgtcaccac cgcgaatatc acccggagtc gccagaggta
     6901 ttcccagctc ctccggtttg atttcggaga agctcgttgc accattcctg acttccgcga
     6961 ccttcgtact ccctgtcgtt gtgatttcgt ccaatccgtc cgaaccgtga acgacaaacg
     7021 tgtgctccgt tccgagaaga gccgctgctc tggcgacgag ttccaccatg tccgcacggt
     7081 acacgccgat gatttgccgt gaagcccccg cagggtttgt caacgggcca agcatgttga
     7141 acacggtagg cgttggagca agctgacggc ggacctcaag agcatatttc atcgccgaat
     7201 ggaacaccgg agcgaagagg aatccgatac ctgtttcgtc gagacacgcg cccacccgct
     7261 ccggggtaag atcaatccga acgccgaggc cactgaggag atcggccgag ccacacctgc
     7321 tggtgatcga acgattgcca tgttttgcca cgggaaggcc ggcccccgca accacaaatg
     7381 ctgccgtcgt cgagatgttg aacgtgcccg atcgatctcc gccggttccg cacgtatcca
     7441 cgagaacgcc atgccgcgtc gggattctgg ttgctttgag gcgcatcgca tccgcggcgc
     7501 cggcaacctc ttccaccgtt tcgcccttgg tacgaagccc gatcagaaat cccgcaatct
     7561 gaacctctgt ggcctcaccc gacatcacct gagtcattgc gtccgaggtt tcagcccggg
     7621 tgaggtctgt ccgactggca agtctctgga tcgcttctga aaccgtcatt tcgcttccgc
     7681 cttctgccgg gactccgtga gtactgcgac cagttctttc ggcgaggaca ccacgatgtc
     7741 tatgctgccg ggtgcaatct cgctttcgga catgtatccc cacgtcacga acaccgtccg
     7801 cacaccggca gcgcgacccg cgctcatgtc gttatccgta tctccgacca ttgccgttct
     7861 cccaggcggg caccctcgtc cgacgcgctc aatgaggaaa tggagaacgt caggctccgg
     7921 tttaggccgc ggcacagcct caggcccgac gacttcggcg aagtacggca tgagtcccaa
     7981 ccctctcact atttgccgcg tctgctcctg aggtttgttg cttgccactg ccattgtaag
     8041 gcccgtggca cgtaagtcgt ccaacatctc gataattccg gggaagggtc gcgtgagcac
     8101 cgtgttgctt tttgtgtagt agctcctgta acggtcgacg attttttcgg ccagcggcaa
     8161 ggcttcttcg ttcggaacaa tcgccctgtg aaacgctgtg ccaccaggcc cgatcgcttt
     8221 caaaagtttc tcccgtggca cgggtggcaa cccaaaatcc tccattgcct gattcaggca
     8281 acgcccgatg tcaggtgccg tgtcacaaag cgttccatca agatcgaaaa caacgagatc
     8341 gaattgatgt ggcatacgat gtccttaacg gttcggaaga gaaaggaaat tggccagtat
     8401 cgctttcccc tgggttgtca gaatgctctc tggatggaat tggactccct cgatggcata
     8461 ctgacggtgc cgcagtccca tgatcacacc gtccgtttcc gcgctgattt cgagacactc
     8521 cgggagcgcc gtcgggtcca ccatcaacga atgataccgg gttcccgtga aagggttcgg
     8581 tattttgcga aatacgccct tcccgtcgtg ggagatttcg gacgtcttgc cgtgcatcag
     8641 ttgtggcgcg cgaactattc gtcctccgaa tgcctggccg atgacttcgt gtccgagaca
     8701 aacgccgaga acaggtagtg tcccggcgag cgctcttacg agttcgaggc agatgccagc
     8761 ctcgttcggt gtgcacgggc ctggagacaa cacgatcctc tcggggtgga gcgactcgac
     8821 gtcgttcacg gtgatcgagt cgtttcgcag taccacggtc tctgccccaa gttcgccgag
     8881 gtactgaacg agattgtagg taaacgagtc atagttatcg atgatgagaa ccatgttaag
     8941 ctgtccccga gagggttcgc gtattgcgta aactgataag atatgttatg tcatgtattg
     9001 ccatgcgaaa ggttcaacgc atttctgcca tctcaattgc gctgatcatc gcccgtgatt
     9061 tgttgagtgt ttcctcatac tccgaggaag catcgctgtc ggcgacaatg ccggctcccg
     9121 cctcaatgaa cgcgaatccg tttttgacca ggaccgttct gatagcgatg cacgtatcaa
     9181 ggttgcctgc gaaatcgaag tacccgatag cgccgccgta cactccgcgg cgcgtaggct
     9241 ccagttcgtc gatgatctcc atcgcccgca cttttggagc acccgacagg gtgccggcgg
     9301 gaaagcagga gaaaaacgca tccaatcggt cctggtcctc ccgaaggcgc ccattgatgt
     9361 gggagacgag gtgcatcacg tggctgtacc gttccaccac catgaattcc gacaccttca
     9421 ccgtccctgg gattgccacc tttcccacgt cgtttctccc gaggtccacg agcatgatgt
     9481 gctcggatcg ttcctttggg tcggcgcgga gttcgtcttc gagatgtctg tcttcatctt
     9541 cggtggcacc gcgtcgccgc gtgcccgcaa tcggtcgaac atgaacgacg ccgctttcga
     9601 cgcgacacag caactcaggg gaggcgccga ggatttcggt gccgtccagc gcgaggaaaa
     9661 acatgtacgg agaggggttg acaatccgga gccggcggta gatgtcaaat gctccagaag
     9721 cgatcttcac gcggaagcgt tgagccagaa caacctgaat gatgtcgccg gcacgtatga
     9781 attccttcgc gacttccacc gcgcgttcga actgctcgcg agtcatgttg gcggatagtt
     9841 ctgaaatccc ggttccccga acgggccgcg ggttcaccgg gtatgcaagg agcttctcga
     9901 ggtgagcgat ttttcggacc gcggcgtcat aacgcgcgcg caattccgac tcgtcatccg
     9961 tcaaccgcac gttcgatacc aggatgatct ggtgggcgac ggagtcgaac accagaacgg
    10021 tgtcgtagaa aagaaagacc gcatcatcga gcccgatgtc gtccagcccg gaatccggaa
    10081 tcttttcaac ccatctcacg gcgtcgtacg cgaaataccc caccgcgcct cctgaaaagc
    10141 gcggcagacc ctcaagcgga atcccgcggt gctggtggag caactcccgg aaatgggaga
    10201 tcgggttacc gttccttacg tcgctggtat tggaacgtcg gtcatgtgct tccgatcggg
    10261 tgccggatgc agagaacttg agaaagggat ccacgccgag gaagctgtag cgtgcgaatt
    10321 tttccgcgcc ttcgacactt tcaagcaaaa aagagtacct atcctccgcg cggatcttca
    10381 tgtacgcgga taccggcgtc tcagtgtctg cgagtattcg gcggtgcacc gggagcattg
    10441 cgccggcatt caccagtctc ttcgcttcct caaaactcgg cgcgatttct gattccgaca
    10501 tgggtcacac gcttcgaaat tcgtacaaca aaaaagctgt gagcgacgtg tgcgctcaca
    10561 gcctgaaacc attgccctgt acagaggtca ggttaccgca cacgtctcct tatgaaagcg
    10621 gcgccaatac cagcgctgtg catccatggg gaacgagacg tgcatcgata ccttcattct
    10681 gaagcaggtg gaaattgata atagggctcc gatactcttc cgtcaagggc ggcgcaaaaa
    10741 aaaccgttac cctctttggc ggcgagtgat ttcccggtgc tccgtacacc gcagcaccac
    10801 catattccca ccgcagttgg taccttagcg caaccaccgt gcgatgatgt tcacaagcgc
    10861 cgttgccaca atgcttatga gcagcatggt cgcaagaggt acgtacaccg tgacgggccc
    10921 ttttttccac acgagatcgc cgggcaatct ccccaaccag ggaaatttcg gtgagaccat
    10981 gaaggcgagc ccgacgatta ccagaatcac tcctgcgacc actatccatt tgcccacgtc
    11041 actcacccga tgtcaacctc cgtcgtgaag tcagttcagg tccggcttca ctctccaatt
    11101 cgccttaccc gccagatgat ctgcaggcgg tgcccttcgc aatcgttgaa aaatgcaatg
    11161 cgtgtcttcc cgaagaacgg attggtcaat tcaggttcca gaaccacccc ttttgctctc
    11221 agatccgcgc aagccctgtc aaaatcatcc acccagatgg caagatggtt tgcgttggcg
    11281 tctaccgtgt tcccatcttc aaggggtggc acgatttcga tcgccatgcc tttggaatcc
    11341 tgtacgaaaa acgctttcgg ctccttttcg ctctcataca cgattttgaa tcccagaacg
    11401 cgttcatacc aattcgccag cgtgcgcgtg tctttcgccg caagagcaac gtgttccatc
    11461 gaatacatgc ttgtttccct ttccgagatt gatgtccaga cgactccacc tccgaatata
    11521 cccagcgacc cgattttcgg gcaaaaccct gtttcacctt gccttcgcgg tgacgggatg
    11581 aatcacgtat tatcttagcg gaagtagcgc ttatgcgtac ctctgcgtgg tgaagtcttt
    11641 ccgggagcag atcaatgtgc gacgcctggc aaaggcattc gagcgatgcc caatcaacgg
    11701 acgcgttggg cggtggcaac ttcattccgt ccgacgagca ccagagtgga tcaaagcaac
    11761 tcccggattc tgcggtgttt ttcgcgctcc tcgagtccta tcccacgggt gttttggtgg
    11821 tgaaccaatc gggcgtaatc gttgcggcaa acgcaaaggc cgcgcggttt ctcaaaggcg
    11881 tgcatctgga cgtcaccgga acatcgtttc tggattttct gagcgcggaa tcatggcagg
    11941 ggtttgaacg cacggcgaaa acactcgttg acgcggggca gggccaggcc cggttgcccg
    12001 tgaaaggcgc caacggagtc atccatgtgt tcgaggcggt ctcgaaatac ccgacgacgt
    12061 cggacgaaaa tgacaccgtt ttgattctgc gggacaccac tgactacgac atgactttgg
    12121 cagcgaacgc tgaggatcgg ttgcggatac tttcccgtgc cgtcgaacag agccagacgg
    12181 tggtgttgat tacggacgcg gaaggaacaa tcgagtacac aaacgaagca ttcacggcaa
    12241 tttccggcta ttcggcggag gaagcaagag gcaagaaccc ccggatactc aaatccgggc
    12301 acacaacgga agcagaatac cgccacctct gggacactat ccgctcggga gagacgtggt
    12361 acggcacgtt ctacaaccga aggaaaaacg gaacgttcta ctgggaagcc gccaaaatat
    12421 caccggtccg aaacaacgaa ggtgtaatca cgcatttcat tgcgctcaag gaagacatca
    12481 ctgcccgcgt ggaagcagaa gaggagcttg cccgaagagc gcgctatcgc atggccatcg
    12541 tcgaagcgga tcgcatcctt ctggagaggg aatccgggga ggacctgaca ccagttctca
    12601 gagtgctggg cgaggcgtcc gaggccgcac gagtgcgcga gttccacaat caacgcgacg
    12661 aatccggcgg gcttttcgca agtccggtgg cagaatgggt ggcgccgggt gtgcagttgc
    12721 aaatcgggaa tcccgcgctg aagtacctgc cctacgaagg cacgttttcg cgctgggaaa
    12781 agacgttgag gagtgggagg ccgattttcg gatcagtgaa ggatttcccg ccggaagaac
    12841 agcgcctgct ggaggctcag catatcgcag ccgttgccgt attcccgatc acaaagggaa
    12901 ctgaatggat cggcttcatc gcgttcgagc gatgcgacga accgccgcag ccatggaccg
    12961 aatctgaaat agcgctgctg ggggcggcgg cggcgtccat ttcactgggg gtttcgcggc
    13021 aggcggcaca gaaagctctc gtcgaaagcg aagaacggta ccgttccgtg gtaaacagcg
    13081 tgaaagaagt gattttccag acagacgcgg agggaaactg gacgtttcta aatcccgcat
    13141 gggaggggct taccgggttc acagtcgatc aaagcattgg gacgagttat ctccagtaca
    13201 cgcatcccga ggacaaaacc gaaaatctgg aactgctcgc ctctctctcg agtggagaac
    13261 tggagtccat cagacacgag acgcgttatc tgacaaaaaa tggcgaagag cgatggtgtg
    13321 aagcgctcgt gtgggtgctt cggaatgact ccggcgccgt tcgaggcgct gccggagtga
    13381 tcgaggatat caccgaacag aagcttgcga ttgaggaact ccgccgggcg aaggaagccg
    13441 cggaggctgc gaaccgggcg aaaaccgagt tcctcgccaa catcagccac gagattcgca
    13501 ctcccatgaa cgggattctc ggtatgacag aactggcgct ggaaacccaa ctttcgcgcg
    13561 agcaacgaga ataccttcaa acggtcaaaa cctccgcgga tgcccttctc gtgcttctga
    13621 acgacatgct ggacctttcg aagatcgaag cggggcagct tgaacttgag cgcatcgagt
    13681 ttgactttcg cgctacggtg gtaacaacgg tgaacacact cctcactcgc gcggaggcaa
    13741 aaaacataac cctggacgtc cgggttgacc cgaacgtccc cgctacagtg gtcggagatc
    13801 ctgctcgact acgtcagatt ctgctgaatc tggtaggcaa cgcggtcaaa ttcaccgaag
    13861 tcggtggcgt ttccgttgaa gtcgtcgcgg agaacggcgg aaaaccgggt agaaccagca
    13921 ttcgagcgct ggtgcgtgat acaggtatcg gaatccctgt tgaacggcaa tcagcgattt
    13981 tcgaaagctt cacacaggcg gacgccacca ttagtcgcag attcgggggt accgggctgg
    14041 ggctggcaat cacgaagcgg cttgtgaatg cgatgggcgg cactatcaac gtcacgagca
    14101 caccggggca ggggagcacg ttcgagttcg tcatcccctt tgagaaaggg agcgcgcgca
    14161 gagaacgccc cgcccctggc aaccaggctg attcgttgtt ggttcgcacc gttgccgata
    14221 cgcagaggga cgggaccact cgaggggacg aaaagggatc gccgaacgag cctcatggtc
    14281 cggaggacgg tgacaccggt tcagaagacg cgcgccgcgc taccgcgctt gtggtggaag
    14341 acaacgctac caatctggca ctcgcacgga cgattctgga aaaggcgggt taccgggttg
    14401 ttgccgccgg cgatggtcac caggcgctgg cttacctgaa ggctgttcgt tcgccatccg
    14461 atcgccccga cgtcattttc atcgacattc agatgcccgg aatggacgga attactgcca
    14521 cgaggagcat tcgcagaatg gatacgtgga aggaagtccc gatcattgcg atgacagcgc
    14581 acgccatgaa gggcgaccgc gagaaatgcc ttgaggccgg aatgaacgac tacctctcca
    14641 agcctgtgaa aaaagaggaa ttgatcgagg cggcgaggaa atggacgcgt acgggccgga
    14701 ctcaacgaga aacgtcgcca cgcacaagag gcggggaccc gtcggttgac acggaggttt
    14761 tcgacgccgc gcgccttctg gacctcgttg atggtgatga aacggcgttc cgggagatca
    14821 ccacaacgtt ccgcgaaacg acgcgcgacc tcgtcagccg gttgaaggaa gcgaacgaaa
    14881 acaaggacgt cgaggcgatg cgccgggttg cccacaccct gaaaggttcg gcgggaagct
    14941 tctttgccgg ccagttgcag agggcaagcg cgaggctgga acaggtgtgc actgatggca
    15001 cgttcgagga aatagcgcgg aacgtcgcga ccgtggtaga ggcttggaat cgcctggaag
    15061 accggatgaa tgccggcaac cgggcagaat agaaacccga ccggggaggg aggaaacccc
    15121 ggtcgggatt gggtcgtacc cgaaggggaa gtcgagaacg acctaagcag gttgtatcta
    15181 ctctctataa cttccgacct ttcgattggt tccttgcagt agttgcggcg cttcagtgcg
    15241 gcgttggccc catgatgcgt aattcgaaga gagggcgtct ccttgtcggc aaaacagatc
    15301 gttgcggtgt tcatgggtgg tcgcgcatcc gaacacgaca tttcgttgaa gacgggcacg
    15361 gtggtcatga actcgttgga tcgtacgcgg tacacggtct accctgttca gttgtgccgt
    15421 gatggggggt ggattattcc ggcggagccg ttttccggaa ttgcgccctg gagcccgttc
    15481 gatgaagccg cgggagcggc gccgatcgag gtggctctcc gccaactgag gtgcatcgga
    15541 ccggatgtgg cattcattgc gctccacggg cggttcggcg aggatggatg cgttcagggg
    15601 cttctcgaac tgctggatat tccgtacacg gggtccgacg tgatgtcatc cgcactcgcc
    15661 atggataagg tcagatccaa gcagattgtc gcgtaccatc ggatcaggac gcccaggtgg
    15721 atcgtggtgc aagaacagga gtggaggctg caacgttccg agatcattgc gcgcgttggc
    15781 cgtgaattcg ggttcccctg tgtggcgaaa gtgcctgaag aaggctcaag cttcggaatg
    15841 gggataccgc agaatgagac tgagctcgag aaggtcatgg acgagagcat cgttgctcga
    15901 ggacacatga tggtggagga gtacatctcg ggaaccgaga ttacgtgtgc tgttctgggc
    15961 ggattcccgg gtgaggcccc catcgctctc ccattgacgg agattattcc caaaaactcg
    16021 gcatatttcg atttcgaagc gaaatacacg cccggggctt cagaggaaat cacgcccgcg
    16081 aggctggatc cagaaatcac ggccctcgct caggatatcg gtgttcggtc ccacatcgcc
    16141 cttggctgcg gggggatgtc ccgaaccgat atgatagtgc gcgaccggga agtattttac
    16201 ctcgagacaa acacgattcc cggtatgaca gaaacctcgt tgtatccgca ggcagcccgc
    16261 gcgatgggaa tggatttccg tgacctcctt acccggcagg tggaattggc catcgcgtac
    16321 caccggcgaa agagggcgtg atcgtcgtgt gcatccaatc agagggctac ttctggccgt
    16381 ttgttggcag agagcggagg catcccaaga tactggaagg tgactgatcg aacacgtcga
    16441 tcgtcagcat catacaccag gatcaaccag tcctcaagac gatgtgctgt tcctggcggg
    16501 gggatcttgc cgagtctgtc tatcaggtat cccgcgactg tttcatactc accttccggc
    16561 agcacgacac cgagcgcacg ggcgattgaa tccggttgcg cggtcgcggc cacgaggaaa
    16621 cgtcgctcgc tcagggcttt gatttccggc tcttccctgt caaactcgtc ttcgatggcg
    16681 ccaaccagct tctcgagcag atcctccatc gtaacgagtc cggtcacatc gccatactcg
    16741 cccacaacga tcagcagatg cgtgttgtgg cgtcgaaacg catccagtaa tcgactgact
    16801 gagatcactt ccggaacaaa aaccggcgga cgcatcaggt cgctgaccac aaccgcggtc
    16861 ggcccgacgc ccgattcata cgtcaacagc aggtctttcg cgaagaggac gcccacaatc
    16921 tcgttttcat ctgcacctgt gactggaata cgggagaaac ctgatgacgc acacaggctt
    16981 ttcgctgttt ccagggagtc gtgccgggcg agcgtgacca tctgaacgcg cggggtcatt
    17041 atctcgcgca ccatagtctc ccgcagcttc agcgcttcgc gcaatagcgc gtgctcgtcc
    17101 gtcgcgagag aaccttcctc accggccatg gctaccgcgg cttccagctc cgaccgcact
    17161 ggagaatgcc gcggttttcc ggccatgacc cagcgtgcga ccccgcgcgt gattccactg
    17221 aatacccaca ccagcggcca gagaatggaa ctcaaagcga ttacggggcg gatcaccacg
    17281 agactggtgg attcagaatg gcttttcccc agttgtttcg gcaatatctc cccgaaaatc
    17341 aggatcacgg tcgtcatgag ggccgtgttc acccagacgg ccgtttcagg gtcggcaatg
    17401 tagtagctga gtaccatcgc ggtcacagac gccgcaccga tgttgacgac gttgttcccg
    17461 attacgatgg cggtcaggag gcgatccgag tgccggtccc actggaggag aggctttgcc
    17521 ttacggccgt agtggtcaag cgcgcgtctg acccgcactt tggtcatggc gacaagcgcg
    17581 gtttctgaag cgctgaaaaa ggcggaaaga cacagaagga cgaaaaggac cgcaaggctc
    17641 gttggaacgg acacggtctt tcttactcct caggcgccgt cagggatgaa gactccgtgc
    17701 cctcagaaag gcgagaagct ctgcgggctt gtctgtttcc aacgcatcgg ctccgcattc
    17761 aattgcctgt ccccacccgt ctggcgtgtc tttcgaaagc acgttacaga acacccgtgc
    17821 gcctgcgcgg tgacaggcgg caaccttcgc aggcgtaata tctcgcagag acgcgcagaa
    17881 gatcgccggc tgcactcgtt ccatgatctc cggcagcgtg ctgaggtcct ccggtggtcg
    17941 cggcaacaga cggaatgccg ctggttccaa cgctcgcagc tccgcaagcg tacgtccgtc
    18001 ggagtacacc gccgtctgct cgcttactcc ttgccgacgc attgcttcga cgaggtcgtg
    18061 tgcgtcaatc ccgcccgcat cgagataaat gcgcacgcgt ccccgcattg cggcgagggc
    18121 ttcgtcgaga gtggggattc gaactcccgc ggccagcgac ccgtcgggat tcttcacgtt
    18181 gagggcccgg acctcggcca acgtgagatc cgtgactttt cccgttccgt ctgtcgtgcg
    18241 gttcactttc tggtcgtgca aaattaccag cttccggtct gccgtgtaag cggggtcgat
    18301 ttccacccaa tcggcgccaa cggcgattgc ctcctcgatt gccggaacgg tgttttccgg
    18361 ccgattcaga tgggcacctc tgtgagcgat cacctcaata acccgggggg gaacgttctc
    18421 gcgcctcagg ccttttgcgc ggagaaatgc gagaagttcc gccggtctgt cagtttgaag
    18481 gccgtctacg cccatttcca acgcacgttg ccacccttcg ggagcgtcct ccgccatgat
    18541 gtcgacgatc accttcgccc cacgtgaatg acatgcgcgt accgattcgg aagaaaggtt
    18601 tgccagagaa gagatcgcaa cgtgatttgt cggtatgctg tcggcaagtg cgtgccatgc
    18661 ggagacatcc cccgggtatt caggaagcgg gcggatgcgc ggctcacgct gaatcatctc
    18721 cctcaactcc gccgggtcgg catacacaac cgaaacctcc accaacccgt gccttgcgag
    18781 gagatctgca acgctatctg gaggcgcgtc tttgcagtcg acgtatgcgc cgacgtgccc
    18841 acgcatgcgc gcgaatactt catccaccgt gggaattgat gctccggcga atgccgagga
    18901 gaacttgctg cctgcatcga gcgccttgag ttccgtcagc gtgaggtctc gcacgcggcc
    18961 ctgtccgttc gttcgagcat ccaccgtgct gttgtgcagg catacgaagt gaccgtcccc
    19021 ggttacgcgg atatccactt cgatgaggtc aacaccgagt gcgatcgcct gttcgatcga
    19081 ttcgacggtg ttctcgggac aggattggtg cgcccccctg tgggcaacca cgaggatatg
    19141 tcttcgcgat tcagttggtg gttccacgtt cgtccttaga ggtgtgcacc cgctcgtcag
    19201 taacaggagg cagaccccgg ttgcgatgcc cgagaccatc atcgcgaaga acccgccgcg
    19261 cgcagaaatg gacgacgtca tctggtgact cgcacggcga gcatgcgctc gagcgccttc
    19321 cttgcgttgg aggctaccgc atcatcaacg aaaacctcgt acctgccggt acgaagggaa
    19381 taccaaagct tctcaggcgt gttcttctgc atgtgcgggc agaggttgca cgcacaactg
    19441 ggcccgggtg cggctacata ggtgttttcc ggcgcttctg ttttgaaccg gtgcaccatg
    19501 ttgccctcgg tggcaacgat gtaagtcttg cctctgttgg cagtcacgat gcgcaacatc
    19561 ccggcagttc cgcggacgaa atctgcttcg tcgcggatgt ttttggggca ctccggatgc
    19621 gcgagaatct gcgcgtcagg gtgttccttc ttcagggcac gcagggtgtc tacgctgaaa
    19681 agctcatgca cgatgcaagt ggcattccac aggtaaatct ccctgtccgt gttttccttt
    19741 acccagccac caaggtgcac gtccggcacg aaaaggatcg gcgttccgcg tgggaccgag
    19801 ttcaccactt ccagcgcatt gccgcttgtg cacaccacat cgctcagtgc tttcactgcc
    19861 gccgtgcaat tgatataaca gatggtaacc agatcgtccc gccccagttt ttccttgaga
    19921 tgcgtctggt aacgagcgag atcctgtgcg tcgcaaccct ccgccagaga gcaccccgct
    19981 tcgaggtcgg gcatgaatac gtgggattca ggattcagga ttttcgcagt ctccgccatg
    20041 aaatggacgc cgcaaaagca gatgcgggaa gccttgacct ccaccgcctt tcgggcaagc
    20101 tccagcgagt cgccgacgaa gtgagcagcg tcctgaatgt gtccgtcctg gtaactgtgt
    20161 gccaggatca gtattccgtt ctggtcagcg aacttgcgca tctcacgtgg gtctggcgcc
    20221 ggttccaaca tcacgccgtc ttcccgcact atggtgtcaa cagccatcaa ttcgtcctca
    20281 tatcgcttcg ctcagatttc ttgccccttc gcgttcggca agagccttgt gtggtaaata
    20341 taaaggcgta tggaacacga aacggttccc tgagctgccg cgtcgaaggg ggctttgaga
    20401 acaaagaggt aggaaaccgc ccgaaattcg cggaatgatc ccgccgccgc gaacactcgt
    20461 caacccggcg ggaactcagg tccaaccttc gtggtacccc gtttcataca tggcaaccac
    20521 attcccggcc gggctcactg cctgaatgtt gtgacacggc gcgatgatgt acccgccgcc
    20581 agcaccaagg attctgatgt tttcctttac ctcattgcgc acatcgtcca ccgaaccgaa
    20641 cggaagtgtc tgctggttat ccacaccacc gtgaaaaaca agtctgtccc cgaagtcccg
    20701 tttgagggct tctctctcca tcccgttgca acgccattgc accgggttga gaacgtcgat
    20761 tccagcctcg atcatgtccg ggattatcgg acgaaccgcg ccgtcggaat gatggaagac
    20821 aaacacccct gcgctgtgag ccaggtcgat catccgtttc atgcggggca ggagaaacgt
    20881 gtggatctgc gccggggaga aaaggaggct ttcctgggac cccatatctt cggcaacgta
    20941 cgacgaaaga atggatgccg tcgatctggc ggcctgctcg tagatccgca atgtgttcgt
    21001 gtacgcgagg tcaaacaact tgtctagaca gtaatgcacg atttccgggt tctcgacgag
    21061 atcaacgaag gcctgttcca acccgcggag ctggcagtac cgaagaaaag gctccgagcc
    21121 gccgccggca atcgggcggt gttcgtttcc cctgatctgg tcgggaatgt gagagtaatc
    21181 ccaccagtct gcctgcggcc agcggtaatt ggcttcgatc tcctcgacgg accggtaccg
    21241 cgccagaggg ttgtgaacag cctccgtata cacaccggtg gcatattcga cggttcggta
    21301 cctgataccg aagacgtctt cgccttccgg cgccggaggg ccgacatagt tgccaccaac
    21361 gctcagtgtg gcgtcaatgt gaagcttcgc gtgcagttcc tcggtgctgt tcacgccaag
    21421 gtgcgcctga agcttccgcg tggcctccgg ggtagcccag tagtccatgg ggattctgtc
    21481 gggtttctgg cggcggagca ctgcctccca gcgttcgcgc ggggtcattg tttctcgtgg
    21541 catcggcgtc cgactccttc tgatggggtt tctcgatgca agcaaaagat cattgctcaa
    21601 caggggggaa ccaaagcagc cctcttctct cgtgtgccgg gattcccgta cctttcggca
    21661 gcaccagttc tatgactcaa tctgcgactt gggaggtaaa tatggtggac atacctgttg
    21721 gcatgtaccg gcctcatatg cgtgatattc ctttattcgc gttgcccgat ggtttcgttt
    21781 ttcgcgattt ccgcgcgccg ggagacgacg gctccggtga cgccgccgcg tgggtaagaa
    21841 ttcaggcggc ggccgaggct cagttcatgc agataacaca cgaaacgttc ctgaacgact
    21901 tcggagggca tgagtcacag cttcccggtc gaagctggtt tgtcacaacc gagaaaggtg
    21961 aggatgttgc gtccatcact gcatggtggt gggatacacc tcccgattgc gagcttcaag
    22021 gcagagtcgg tctgatccac tgggttgcca caattcccga atatcagggc aggaaaattg
    22081 gaaaggctat gatgacgaag gcgatgcaac gccttgcccg ggaccatcag gtggcgtatc
    22141 tgaacacttc cacgatccga atccctgcga tccggatgta cctggatttc ggatttcttc
    22201 ccgatttgaa aaagaatcgt gcggacgaag cgtggtccct tgttcgcgag tctctgaagc
    22261 actccgcgct cgggaatgcg taactgccac cttacggcgt tgcacgctcc ccgttcatta
    22321 tcaatacgca acgacagggt gccccgtcgg atgtgcgcac acgcaaaggc atgcacacga
    22381 gttcatagcc gccagccggg acctcccgca ggtcgcagtt ttcgaggacg agcacgtctg
    22441 aaccaagaag cgttcggtga acggggagtt ccgtcgcgtc agccgcatca acggacgggg
    22501 aatcgatacc cacgagtcgg accccgcgcc ggacgagcgc cgctgccgcg tcaggtgaca
    22561 gggaaatcca cgataagggc ttgtgcgcgt cgggcggccc gataaagagc gttttgaaca
    22621 gaactgcctc atattcctcc agacgcagag cattcacatc cgccgcggtg atgactcgct
    22681 ccgatttcgc ttccacaacc tgcgccgaaa gaaagaacct ttccggggga tattcgtcca
    22741 aggtcttccc gcccggaaat acatgcgcgg gagcgtcgag gtgggtgccg ctgtggcttg
    22801 acatcgtcaa gcggctgatt gtcaccccgc cacattcttt cgcctctcgt tcaaacgccg
    22861 gatcgccggg gtacggtggg gtatcgaaac cgagcgtgcg agtgacgtcg aagatgcggc
    22921 cggattcaat catgcgtgcc tccgcgctcg cgcgtcggga gcttccaaat cgtgggcggt
    22981 cgtgcgcata acccgaaccc tgtacatgcc ccgcacggaa ttcccgagat aaacaaccgg
    23041 gctcctcaac agctcggaga tcgaaatgac gcgctcggtt actctgccgg tgttgatgag
    23101 gtgagcgcgc attgtgccac cgagcagacc acaggccacc ggcggtgtga aaagccttcc
    23161 gccaatattc acgaagacgt ttgctacggt agattcggtt acttccccct tttcgttcca
    23221 cagtatgaca tctcggcagc gcgggttctc tttcattgcg gattcgtaga ccctccggtt
    23281 ggttgtcttg tgaaagacaa acacatccga cgtgtgtatc ggtttgcggg cgactgctac
    23341 gcgaaggttg ttgccgattc ggcgatcgag ggggaatgct tccagtgaaa tggcccccga
    23401 tttttccacc agcaggcgga ttcgttgtgc ctttcgagga agcgttctcg cgaaggatcg
    23461 gagttcgcgc cgaacttcac gcatgtcaac cttgaagcca aagtagcgtg cactttctgt
    23521 gaggcgccgc aggtgatgtg tcttcaggta ataccctgac cgaggttccc agcgcatcgt
    23581 ttcaagaagc tggaattcgg gtacttccgc ggaaagcacc ttcgcctttg tttcgcactc
    23641 ctcccattcg gattccgcat ctgagtccca gacgattccg ctgcctgcgc cgtattctgc
    23701 cttgcccgtc gttctgtcaa tcagcgcggt gcggatggca acgttgaact gcgcgtcccc
    23761 gccgggagtc acaaacccta ttgtcccggt gtatatgttg cgtcgtgtcg tttccaggtc
    23821 cgcgatgatc tgtgtggtcc ggatttttgg cgcgccggtg atggaagccg gtggaaatgt
    23881 tgcccggaac acgttctgca ggcgctgttc cgttctcgcg gtgaccgtcg aggtcatctg
    23941 ccagatagtc ggatactgct cgatttcaaa aagacgttta accgccacag acccggtttg
    24001 ggctactcta ccgaaatcgt ttcgaaccat gtcgacgatc attacgtttt ctgcccgttc
    24061 cttcaacgag gcctgaagtc gttcggacat gaggaggtct tcctcgagcg tcctgccgcg
    24121 ggctgcagtt cctttcatcg ggcgtgattc aagttcgctg ccatggcggg tgaagaataa
    24181 ctccggagag gcgctgcata tcgcccattc ccccgcgtca atatacccac cgtaaggagg
    24241 attctgggct gcgaccagtc tcaggaagaa ctcccacggg tcagaggtga actccgacac
    24301 gagacggtag gtgtagttca cctggtaggt gtcaccggat tcgataagtg cgtgtacccg
    24361 gtggatggcc tcgtaatact ccgtccggga taccgaaggc cgccaacgga acgcgcgcga
    24421 caacgcgttg gcggggggcg ccgggatatc aaccggttcg acgtttctga acaggcagaa
    24481 ccaggcgagc ggaaaccttc caggagtatg cgccgcaagc cccgcgtcaa acgcgggcgc
    24541 cgcctcgtag gaaacaaacc ccgcgcagta gagggcatcc tctgcagcag cccggccaat
    24601 cgtttcgaga acctgcaaca cctcctgcag cgtggtggca acgagagtgc gctgcgggtc
    24661 aacgaatttc agccactgcc cgtgtcgagc gtcccgcaga attaccctgg gggcgtgtcc
    24721 tgttcctgtc cgattctcag tcatcgcgat gtgtcgctcc cgcgactgat tgcggtcgag
    24781 tacgggcggc cggtgcacgc ttgagcagtt tcttcatcgc ggttaccgca agcacgtcgc
    24841 aggcttcgtt gtccggatcg ccagaatgcc cttccaccca ttcccatctg accgagtgaa
    24901 ccccggtgag gcaatcgagt gcctcccaca ggtcccggtt cttcaccgga tcctttgccg
    24961 aggtcttcca gccattcttt ttccagttgg gaagccactt tgtgatgccg tctcggagat
    25021 accgcgaatc cgtcacgaca accacattgc agggctcctt taacgcgcga agtgcctcaa
    25081 tcgctgccct gagttccatg cgattgttgg tggtctcggg ttctgcaccg aacagagact
    25141 tgcggtgcgt tccgtacgtc agaatcgccg cccacccacc cggaccaggg ttgccggaac
    25201 atgcaccgtc cgtatgtatg cggatttctt tcatcgtacc tcgacagttg ctgcgtgacg
    25261 ccttgggaat ctaaaattcg agaacctttc gcaccagaca aagcttccga cgtgtggtga
    25321 tggcaaccgg acgggacggg ccaaccatga tcgaagacct caaccctgcg ttcgatatgt
    25381 ttctttttcc cgccaaagtg tccgcccgcg ttcggcggta cttcagcctt ttcgatcggg
    25441 cgttggcgaa tttgctggca cattttgacg tggaggcggg attgatccgc cacgcggttc
    25501 ccgaaggttc gcccggtgtc tccgaagaag ttgccgactt ggacaggttc gaacgaaaaa
    25561 agtacatctc gccgttgaac aagtgggcgt acggcgtcgg aaggcagagc gcggccgcca
    25621 tcggtgcgtt cggatatgga tacgcgcaac gcatgtccat gtattcgggg gattcacaca
    25681 ttctgcgtgc ggttcgaaat ggcttggagg ccttcgcgcg catccaggtc ccttcgggcg
    25741 aattcgtctt caccccgata cgattctgct ccgtgtaggg cacccacgaa atggcatggc
    25801 gactgg
//
LOCUS       DAOSOO010000020        25408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1957_length_25408_cov_7.176587, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000020 DAOSOO010000000
VERSION     DAOSOO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 25408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25408
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_25408_cov_7.176587"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLT86_02755"
     CDS             complement(<1..353)
                     /locus_tag="PLT86_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35566.1"
                     /translation="MRMLILLLAVLGLCAGNATCDTLVERSGRELSGSLVGVQDSILV
                     FRTDAGDTTRIARSAVERVTFFAPVHGHLGTVQLDRSGRVELVVFGTGYGILNGALLA
                     SIADFGVDGFTLSMLA"
     gene            598..1353
                     /locus_tag="PLT86_02760"
     CDS             598..1353
                     /locus_tag="PLT86_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35567.1"
                     /translation="MHKSSFFLLSGAAILIMAGASHAAGNSPQRPASSMVAAELPAVD
                     MDTTVFVDADGDGINDRMPRMRRDDLRALDQEREQSRGQMLNQAMAGLTKEQKDEIKQ
                     LRTAMRRQGASPDQIHAAVGEKIRSYGVAVPADWDLTANEYANARRMTAQRRAQVQAL
                     ADSLRAEGKSPAEIRAAIAAEYSPGYAGPRGSKAMGRDTGRARQDSLRAAGRPQMPAG
                     RDSVRQPMRQAGRDTTEARGRAPRPERGGGRRR"
     gene            1775..2692
                     /locus_tag="PLT86_02765"
     CDS             1775..2692
                     /locus_tag="PLT86_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35568.1"
                     /translation="MKYAPFFLVAGAAAFLYAAHVPAQEIDNTGTDVPAWVDADGDGI
                     NDVAAMRRERRGGNENAYAESRRQLMAELTANLTDEQKAELTALRNQLREDHATPEAV
                     QAALGEKLQSFGITLPDAWYQTRLEFEGGFTLTAEQRAQVQALMESLRTDGKTPEERR
                     AAVVELLTSYGITVPDNFMAPPERRNGPRGDRGRGPILTEEQRTAVDALVASMKEEGK
                     TRAEIQAAVGELMASYGITAPVRRGPPEGTQAARSGLTNQQKAEVQALVRTMRAEGRS
                     AAEIRDALAAKFNQYGVAMPPQGKGPRGR"
     gene            2804..3868
                     /locus_tag="PLT86_02770"
     CDS             2804..3868
                     /locus_tag="PLT86_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35569.1"
                     /translation="MKRVLWVLLGSLLPFAVQAGSFSGEIRTLGGYDSNPSGYTGGEA
                     QGLGATQAKLTWRVPLGDASGDFRVTYLGNLSAFTPRSEWTAHSHELSGAFSQDLEDN
                     LNVGFRGGAGGNWNRPAYDAYSYREFFGEGWAQTELDAWPVYASLAGNYRNYPASPSF
                     DFREFTMTAYTSRYWATRTTFRLTFSATERTYITQTIEDFFAGQTNATSSQFQISALA
                     SQGLTDRSGLRFSGWALSGSGRTRWRDDYLEAMDDPLATRGVGGRVQFSVLAPLSLTF
                     RTYLGGQRTTQAYITQTGEYGDHTDTIREAGVVLEGKLPRISTRLSWSTELNAQRRTS
                     SDPTYAFDRAALTVGVKYAW"
     gene            complement(3896..5950)
                     /locus_tag="PLT86_02775"
     CDS             complement(3896..5950)
                     /locus_tag="PLT86_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35570.1"
                     /translation="MADADNDRGFWKKLTGRGRAEGDRSTQEDKLAAERKKYGMDPEL
                     EMAPYESGLTARTLIGALFVAIVMMPASIYLGLVVGTGLGSAAEWVSIILFTELARRS
                     FMPLKRQEIYILFILAGGLVGGGPFGGLIWNTYLQSSQEALSLGVAQRLTTENIWWAV
                     PPAGSEAVALRTFWHKDWLPIVTLMVFTSLISRFQTLSSYYFLFRLTSDVERLPFPFA
                     PIAAQGITALAEGSEGETWRWRWFSIGSMIGLVWGAIYVGVPTVTAGIFGTAIEILPI
                     PFVDFTREIGNVMPGSLLGFNTDLGGIMWGFVVPFPIICGQVISAFLSSFVLPPFLVE
                     AGILKSWRPGLGVVQASVLNNLDLWTSVSLGVGLPIMFLGVYESTKVLMRQRDGRGAG
                     TFAPPPGRGDLPLWLAAAIWFVTSAFFVGLAHWLVPLFPVWIYILFAFVYTPFISYIG
                     ARMIGHAGTHVAFPPITQAAFILSGYKGIAVWWVGSQANDFSGGVSKFREIELTKTKF
                     TSYFAVEFIMFPITFILSFAFWSVIWKMNPIPSSAYPFALKMWPLNAYNACLWQTATI
                     QGNSTMLNAIKFDVIGYAAIGGGVLYAIIHFLHASPMWWYGLIGGLGAWPHGIFLSLT
                     GALLSRYYLAKKFGGPEQWFKYAIVLNAGYACGVGLIGMAGVAIALINGAITQSPFQV
                     FR"
     gene            complement(6007..11751)
                     /locus_tag="PLT86_02780"
     CDS             complement(6007..11751)
                     /locus_tag="PLT86_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT35571.1"
                     /translation="MTRIRLPFLRGTAYGVRGAAKITFFVACLLGAVSTAGAQYRRDP
                     SAVELGEIGSRHAAQIDSARFVRSWELLSRYPTREIGTAGNEAATDYIAATFDSIGMQ
                     HVTRQRVTVADARQQYAYAVLPLEDGTFDTIPLRCYAPNFAHTNTIPPGGLKGHLVYS
                     RTGEAREYNGHEMEGSVLLAEMTVEERWRLASTLGSKAAIFIEAPKVTISAQFYTSVN
                     FPRFWVNRQDGDSLRAWAAAHPNTEITIHAKQPWRMLTGNSVTGFLPGSDPVLSREVV
                     IITAPFDAGSNVPGLAPGAEAAFSSAAQLELARIFGKERPPRSLYFAALNGQRSGVME
                     GGRVFVQALKQSYPGALRQNLERLRADLRLISDRKLSGRLPAHRERALALERRLGDVV
                     GNELATAQRYWDDLQNARTEYLKGGAVAEKRTGGALYYARRVRVGAMEEAAERFKPAF
                     KRALDACEPLIADYGRLLEDFVGELENDRARLKPIVDADEGKIRTSVAGRLAAARMTD
                     ISKALQKFTRMSPDDHTTRLERLRSDLALIEPILGRYPALLIDLSLTSNSNNIGLFHK
                     GYFYNQQPDLELQRDYGPLGKRFMGYATNLVVVNDLNGLWSRSDEQIRALILAKIAEI
                     SAKAETYRKVAGIALSDTEVNYSYDWYQRFVNATAWAWGLLLAGVCGWLAYRRRRADE
                     TSITPAWWQIAGIVAGLGLTGYALIAGMDWKNTEKIHHGIDQLIWNHTDLLINAEQIP
                     REEKTKIRLAAERHKRYVEMVEAPEFFAENIINAVPATLNKLRQDSLIIAQQLVMFQK
                     IAPWRTIPPAQLTQGQLDTLNQFIGFLGLQGDRRFVDGVAGTPGKAWWNFLPAARYFT
                     SEIASLAGIPAIALSTTGDIAPELSSLRDTFSRVNIPGSLTQMKTLTAITGQALQDPL
                     LPDDMVMGDYFATSSGLVLYDDRQASVTADQPVDSAVVIADRGGYRFTTITNRRGAFE
                     FVGLPLDGRAAARSADPQWLHAYKTSADSGNVRFAIDLGSKQYAFVPSVSQLSQDWTV
                     VVFPCASVSVTDVLDPRYFQFLARGDIYEARTDSKPWHYFSHVAMGDNTFFVEEGTRV
                     KIIFAQGLLGVRMMLTGTPDSIQIPEHRQGLGFKVEREALLTHTAYQAARDMWQLNEG
                     RLVTLVRHGIIDAETRAVHDSSAYRLALAKKALEEKRYDTYLTEARSALALESRVLPD
                     VLGIANGALKGVLFYMALLLPFAFFVERLIIGSTDVKKQAIWFFAIFILMFVLIQQVH
                     PAFDITLAPPMVLLAFTVFALSLFVVSIIIGKFTAQMKQIKKEQQGYLSADVGRVSAM
                     FAAFLLGVSNMRKRKVRTTLTCLTLILLTFTVLSFTSVQELLRVNKVRLSAQKPTYSG
                     VLIRDRFWNFWPRETLTSIRNEFGQENIVVGRGWREALIQGATSNLFTLSAPNGKSYT
                     LSGFVGMEPQEDEVTGIFRKTGVAGRWFQPGDFRVLVIPQTAADALGITRDQVTGDED
                     TAPQIIMDGVRYGVIGIVDDELLRQLKDIDGEELTSVNWALVETKRQDRISQVVQAQK
                     APTAGERDSFIHLVPAQCMFMPLNLLPGGLVSVAIKTPDSNTALGLVNRVIPKWALEM
                     YVGQNEGTFLYSAIGLTTLGGLKDVLIPILIAALIVLNTMLGAVFERTREIGIFSSVG
                     LAPSHIAMLFMAEAVVYAVLGAIAGYLLGQTVGQVIYKTGLLGGVTLNYSSLSAIVST
                     LIVMITVILSTIYPAKKASEISVPDIARTWKLPEPNGDEWFFALPFTLMDEELEGVNA
                     FLAHFFESHRDESASDFYIDRVDFWADSGRFMLETMVWLAPYDLGVSELVTLISSPVP
                     NEKGLFGMSLLVTRESGDVPSWNRVNRRFVNYLRKQFLIWRTLTPAEREMFNERGRAM
                     YSVNGERAISQKTT"
     gene            11929..12726
                     /locus_tag="PLT86_02785"
     CDS             11929..12726
                     /locus_tag="PLT86_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT35572.1"
                     /translation="MAEKTLKGRTALITGSTSTGMGRSIALRLAAEGANIVLNYGTHR
                     DFSTHDGGADAQARAEEVASHIRRLGGQAIIAPGDTRSEEDTIRMVDAAKKAFGAIDI
                     LVLAAGGKWKPQPLESVTIDFWRDVMAAEVDAMFLAFKHVIPGMRARKFGRIIVFSMN
                     GAMTRKTLEATAADYTIGKTARTWLSLAVGHDEFENGITVNIIEPGPVGHMTLNEALA
                     AIEHTDNAWEQRAKPVAHDAAELAAFLCSEAGRFVSQSLIRYPTDGW"
     gene            13346..14146
                     /locus_tag="PLT86_02790"
     CDS             13346..14146
                     /locus_tag="PLT86_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT35573.1"
                     /translation="MPPKLNRSPILCFSHIKGGVAKTTCCVNVGAGLADMGYRTLLVD
                     MDPQSSLTDVFLGNGEPPAASLYQVLYADGPGQRQMAISDVIRPVRENLDIAPASLDL
                     VRLEHLLLPEVDGRDRLRRALRGHGYDFVIIDTSPSVGILTSNAMIAATDLIIPVQAS
                     YFALKGIENIMETFNTIRERVNESLNLLGVVITMVDKRKRHPREVIADLVEIFPRELI
                     FDTQIREVAKVEASPGYHQDIFSFAPESPGADDFRAFIQEILTRCRNS"
     gene            14131..14943
                     /locus_tag="PLT86_02795"
     CDS             14131..14943
                     /locus_tag="PLT86_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT35574.1"
                     /translation="MPKFVTKTISLGRTGDNELRNLIAPDKRIGKLVPVDEITPNPDQ
                     PRRHFDPDALEALKESIRQHGLINPITVDENGRILAGERRYRACVALGMREVPVIRMS
                     GSLEVSLVENLQRQDLHPLEEARGYQTLVDAGYTHEQIATRIGKDRSVVTHSLRLLDL
                     PADIQAECVTSHNVTKDQLLQILGADSPEQQRRIWEDIKAGKSARALKQERSEAKRRR
                     QITPRVFINRVRSFSTDAGKIDVARLSDTEKRALKTHIEHALARLSSLLGEL"
     gene            complement(15078..16211)
                     /locus_tag="PLT86_02800"
     CDS             complement(15078..16211)
                     /locus_tag="PLT86_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT35575.1"
                     /translation="MKYRRFGKTELMVSVFTFGAMRIPDGTDENAVATIRRAVELGIN
                     HLETARGYGSSERLMGLAMPSLPREKLIITTKIGPADTADEMRRSLDESLERMRVDRI
                     DNFDIHGINNREILRRTLKKGGCLAAIRKAMEEGIVGHLGFSTHAPLEVIMEAIGTGE
                     FESVNLHYYTFNRRNLPAIEKARSLDMGVFIISPTDKGGQLFNAPAKLRELTKPYTPI
                     QANHRFLLGNPEIHTLSLGAASPHEFEEHLAVADNDGPWTEEEAALFARLDEEMRRAL
                     GTTFCTQCYECLPCPENIHIPETLRLRNLARAYDMKEFGKYRYNLFSRGGHWFPGEQA
                     TACTKCGECLPRCPEKLDIPALLMDTHELLLGDPQGHLYARND"
     gene            16414..17463
                     /locus_tag="PLT86_02805"
     CDS             16414..17463
                     /locus_tag="PLT86_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT35576.1"
                     /translation="MQPVQVSSPVAGDVGRNRLARRILNNALASIIEPVRPHFDSVLR
                     QLDSILTAEVPTVDAVSRYVAATKGKCIRPALVLLAARCVGAITHEVRLAAVSMELLQ
                     ASTLLHDDVIDQSVLRRGVPSVNARWDNETAVLMGDILFTQALSTMLETGSIPVMKIA
                     ALQTRRMIEGEVHARDKRRSPVFSEESYLDLINRKTASLMSLATEGGARLSDGTEQQV
                     NALASYGELLGMAFQIVDDILDVVGDPTVVGKPTGQDLREGTITLPLIRALNAAPHED
                     AQKIRELVSLGVNTDADWNAVHAFIELYGGVASALRTARHYAEQARAHLGILPPSDAR
                     DSLDRMVGYVISREI"
     gene            17486..18148
                     /gene="trmB"
                     /locus_tag="PLT86_02810"
     CDS             17486..18148
                     /gene="trmB"
                     /locus_tag="PLT86_02810"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOT35577.1"
                     /translation="MARISIRSIRYRRPLNPTWDTELEQQIHCQIEGIEAPLDLAGLF
                     QRQAPVHVEIGFGKGRFLLAAAERFPETNWLGIEYAPPCVALAVERAARKGLRNVRIA
                     RGVAEDIIAARFPADTVAAYHIYFPDPWPKKRHHKRRLIKPPFAVQLARTLEPGGFVN
                     TATDNSEYFEEIIAACTGAGLARMDVPDIYGDEAFRTNFEMRFLTHGQPVYRAAFRKR
                     DR"
     gene            18252..18707
                     /gene="bcp"
                     /locus_tag="PLT86_02815"
     CDS             18252..18707
                     /gene="bcp"
                     /locus_tag="PLT86_02815"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOT35578.1"
                     /translation="MPTKGDNAPDFSLADQNGVVHTLKQYAGKWVVLYFYPKDDTPGC
                     TKEACSFRDNLGAVTKKGAVILGVSGDSTASHKKFATKYDLPFPLLADTEKAVINAYG
                     VWKEKNMYGKKTMGVERSTFLIDPAGKIAAEWRKVKVDGHTEEVLAALP"
     gene            19012..20127
                     /locus_tag="PLT86_02820"
     CDS             19012..20127
                     /locus_tag="PLT86_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT35579.1"
                     /translation="MELFRTAFLVLLALGATGYAVASVALWTGALRRSRRIPRGGDLP
                     TVTVIVPARNEAANIASCLTALSRQSYPPHLCKIVVVDDRSDDGTGEIAARAACAMEA
                     VVQVVRVDTVPPGWSPKKHALHRAILESTSDIILTTDADCEPEECWIEEMVASLSAAH
                     VVAGYSPYRDRTSLPARLLALETLSQAFLAMGGIGIGRPITCTGRSLGYRREVYAAVG
                     GFGEAHSMLSGDDHLFLQRAVGAGFTAAFCDGPGSYVWTNPPPTWRGFVQQRIRMFSG
                     ARMLKPAVAALGVFVYAWLAVMGVGMLAAFPAAWGAFGVKVLLDGVSMGIAAVRLHEK
                     RLLAVYPIAAFLYLPYFLVFALWGTVGSYRWKGARGH"
     gene            complement(20124..20897)
                     /locus_tag="PLT86_02825"
     CDS             complement(20124..20897)
                     /locus_tag="PLT86_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT35580.1"
                     /translation="MSVHVKEANGVYVVHPRFARLTATHGAREFRAALEKGVRDGYEG
                     IAIDFSGVKFADREILHVLAAAYKLHSPSYGLMFAFHCSDDLEEFFEETLLDRLIPVV
                     KDEEAALAVLAGCRKRPVKTRAAEKVLGKAYAVGHRIGALPDRRVAAVNVPPEHERRR
                     GARAAAETTGRPDVEETPVTVQPEKAPQRVEEVTPEPLKEPAAPTKQPSRAKRPAKAQ
                     KETEERVLQPGAAEAPKKPKAPARKKTPRAPRKKPEQGG"
     gene            complement(20944..21303)
                     /locus_tag="PLT86_02830"
     CDS             complement(20944..21303)
                     /locus_tag="PLT86_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT35581.1"
                     /translation="MSATVRITRGVVVITPDQKRLTAHRGMPAVRTRLEEAVEQGKKA
                     VAVDFSGVEYVDGAMMGVLIDAFKGIHRNGGRLCVFGAGDAVHEVLKNTVLDDVIEIV
                     SDEDAAIEALKGKKRNL"
     gene            21540..22490
                     /locus_tag="PLT86_02835"
     CDS             21540..22490
                     /locus_tag="PLT86_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT35582.1"
                     /translation="MQVTIWGCRGSLPTPMPKAEYEKKLSDILTAYRDAGAPADIEAF
                     LQSRPFVERSTFGGNTACVEVTEGDHQLILDAGSGLRPLGLKMMGGPCGKAKGDVHML
                     ISHTHWDHIMGFPFFVPAYIPGNKITIYGCHDNLKERFEYQQVFTHFPVLLDQMASTK
                     TFVQVEVGKPFEIGPFKISTIAHFHPGDAYGYRIETCKASFVYATDAEYTEVTWEQQQ
                     KHIEFFRGAGAIVFDAAYSLKEAVEKLNWGHGSPLLGVEMAVQAGAKRLILFHHDPAT
                     PEKQIAEAHAKAKRFYDELPNKTTECEILTAHDYMTLDLG"
     gene            22705..23592
                     /gene="thyX"
                     /locus_tag="PLT86_02840"
     CDS             22705..23592
                     /gene="thyX"
                     /locus_tag="PLT86_02840"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT35583.1"
                     /translation="MADTRRFSNPAADAWIDKPIPCLDHGFVMLKDYMGGDASIVQAA
                     RVSYGEGTKKASDDKGLIAYLMRHRHTTPFEMVDLKFLVKLPIFVARQWIRHRTASVN
                     ELSARYSVMADEFYLPKKEDVRYQSTLNRQGGSDEEVSDELCGRVVETLRAGAERAYN
                     EYEEMIEANIARELARTHLPVSLYTQWYWKINLHNLLHFLELRLDTHAQYEIRVYAQA
                     MSQIVKDVVPWTWEAFEEFRLNAQTFSASEKAVLAMLLQGKEVVLPESLQKGGRRREF
                     EEKLAALGVDPATVLPPAK"
     gene            24225..25352
                     /locus_tag="PLT86_02845"
     CDS             24225..25352
                     /locus_tag="PLT86_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35584.1"
                     /translation="MFYRKDGTKVFPIGMYSFPANDSEWTLWREAGVNLLRCGNRDQL
                     DEAWSHGMFGWVSVPMVLAPGDDGTVLATLIDSLKDHPALAVWEAPDEAIWNAWYDGR
                     IPRRLWTDSPEEIAEKKRKMDDLVDGLTRGAAIVRERDPGRSIWLNEAVLSPLDIVAR
                     CAGSLDIIGFDYYASGIPEDNFAWPMQLMGRDIDRFRAAAPNCAVWMIEQAFSYPELL
                     PQYCSHFPGGTPSRDELRFMAWQAIHHGTTGLFWYGLHKVQRPTPFMDDLMAVVSELR
                     QTVAFVQSGPVPGITAEAHHSWRPSIMGCSCTVWRLGNRVLVFMINEDAHSVDMHIRG
                     LDDVVRGGLVPVNEPSADLTPLDGGFITPMRGHEVRLYLAG"
BASE COUNT         5123 a         8208 c         7336 g         4741 t
ORIGIN      
        1 gccagcatgg acaatgtgaa cccgtccacg ccgaaatcgg caatggaggc cagaagagca
       61 ccattcagaa tgccgtaccc cgtgccgaac accacgagct ccacccggcc ggagcggtca
      121 agctgcacag tgccgaggtg cccgtgcacc ggcgcgaaaa aagtcacccg ctccacggcg
      181 gagcgtgcga tgcgtgtcgt atcgcccgcg tccgtgcgaa acacaaggat cgagtcctga
      241 acgcccacaa gggatccgga aagctcccgt ccggagcgtt cgacgagcgt gtcgcacgtc
      301 gcattgccgg cgcagagccc gaggaccgcc agcagaagaa tcagcattct cattcgaaac
      361 tccccggatc tgtagagacc cgttggacaa ctggaaccag catgcacccg gtgggagtga
      421 gggtacgcac ggatgcaacc ttctccaaac gccttcgcgg gcgtcggcat ttttgtgctg
      481 ccggacggaa atcaccgcta tcttctgttc aggccgttat attgtacggt acgttcattt
      541 ttctggcgca gccattccgg ctgcgctgcc accagcatta catgaaggag tatcgcgatg
      601 cacaaatcgt cgtttttcct tctttcaggc gccgccatcc tcatcatggc cggcgcgtct
      661 cacgcggcgg gcaacagccc ccagaggccc gcgagttcca tggttgccgc cgaattgccg
      721 gcggtagata tggacacgac cgtctttgtt gacgccgatg gggacggcat caacgacaga
      781 atgccgagga tgagacgcga cgacctccgc gcgctggatc aggagcgcga acagagccgc
      841 gggcagatgc tcaaccaggc catggcgggc ctgacaaaag agcaaaagga cgagatcaag
      901 cagctgagaa cggcgatgag gcgccagggc gcgtcgccgg atcagatcca cgcggcggtc
      961 ggggagaaga ttcgttcgta cggagtggcc gttccggccg actgggatct gacggccaac
     1021 gaatacgcaa acgcacggcg catgacggcg caacggcgcg cgcaggtgca ggcacttgcc
     1081 gattcgctgc gggcggaggg caagtctccc gcggaaattc gcgccgctat tgccgcggaa
     1141 tactcccccg ggtacgccgg cccgcgcgga tccaaagcca tgggccgcga tacgggtaga
     1201 gccaggcagg acagcctgcg cgccgcgggg cgtccccaga tgccagccgg gcgcgacagc
     1261 gtgcgccagc ccatgcggca ggctggtcgt gataccaccg aagcccgcgg cagggcacct
     1321 cgtccggaac gcggcggcgg acgcaggcgt tagtatcgat tggagagcgt tctcgacgaa
     1381 gccggggtga actgtcaccc cggtttttcg tttttcctgc ctacctgttc cgaacggtgc
     1441 gtacgcatcg gtacgcttcc ggcagggcaa cggcatcccg tttgccgcca cactcatcgc
     1501 accggcgctg cctccggcat gctccgggag cgcgcggccc cagacagtat tccggacgga
     1561 cgcgatgtcg ccgcgcaaaa taattatgaa caatatctta gttgctttcg ctccgcgtga
     1621 gactccctgt tggcacaccc gttgcaagaa ctgggagccg ggaagcgagg cgctcatcac
     1681 gagcgctgtt tcaccgaaac tggttggcca ccagattgcg cacacagagc aatgggtttt
     1741 cgcaccatca cacacagggt aaggagcacg aaccatgaaa tacgcaccgt ttttcctcgt
     1801 cgctggagcg gcggcgtttc tgtatgccgc gcacgtgcct gcgcaggaaa tcgacaacac
     1861 cggcaccgac gtgcccgcat gggttgacgc ggatggcgac gggatcaacg acgttgccgc
     1921 aatgcggcgc gaacgccgtg gcgggaatga aaacgcgtac gccgagagcc gccggcagtt
     1981 gatggcggaa ctcaccgcca atctgaccga tgagcagaag gcggaactga ccgcgctcag
     2041 gaatcaactc cgcgaagatc acgccacccc agaagctgtt caggccgcac tgggcgaaaa
     2101 actccagtca ttcggcatca ctctcccgga cgcgtggtac cagacacggc ttgagttcga
     2161 aggcgggttc acattgactg ccgaacaacg cgcgcaggtt caggcgctga tggagtcgct
     2221 gaggacggat ggtaagacgc cggaagaacg ccgcgccgca gtggttgagc tgcttacatc
     2281 gtacggcatt accgtaccag ataactttat ggcacccccg gaacggagaa atggtccgcg
     2341 cggcgaccgt ggccgcggcc ccattctgac ggaagagcag cgcacggccg tagacgcgct
     2401 ggttgccagc atgaaggaag aaggcaaaac ccgggcggaa atccaggccg cggtgggcga
     2461 acttatggcg tcctacggta tcaccgcacc ggtccgacga ggtccgccgg agggcacgca
     2521 ggcagcccgt tctggactga cgaaccagca gaaagcggag gtacaggctc tcgtgcgaac
     2581 catgagggca gaaggcagat cggcggcgga aatccgtgac gccctcgccg cgaagttcaa
     2641 tcagtatggc gttgccatgc cgccacaggg caaggggccc aggggacgat aacctctcct
     2701 cccggaaccc gggcaacctg cgagtatcat gagcattcat gcatggtaga cgatcacaac
     2761 cggggcggcg ctgcgccgct ccggcttccc ttcgggtggg acaatgaaaa gggtattgtg
     2821 ggtgcttctg ggatccctgc tccccttcgc agtgcaggct ggttcgtttt ccggagagat
     2881 ccgcactctg ggtggatatg acagcaaccc ctccggatat acagggggtg aagcgcaggg
     2941 tctcggcgcc acgcaggcga aactcacctg gcgcgttcct ctcggcgacg catcagggga
     3001 tttcagggtc acgtacctcg gtaacctgtc cgcgttcacg ccacgttcag aatggaccgc
     3061 gcacagccac gaactgagcg gcgccttcag ccaggatctt gaggataacc tgaacgtggg
     3121 gttccgcggt ggcgcgggtg gaaactggaa ccgacctgcg tatgacgcct attcgtaccg
     3181 ggagttcttc ggcgaaggct gggcccagac ggaactggac gcgtggccgg tgtacgcgtc
     3241 tctggcagga aactaccgca actaccccgc aagcccgtcg ttcgatttcc gcgaattcac
     3301 gatgacggcc tatacatccc gttactgggc gacgcgaaca acgttccgcc tcaccttcag
     3361 cgcaaccgaa cgcacctaca tcacgcagac gatagaggat ttcttcgccg gccagacgaa
     3421 cgcaacgagc agccagttcc agatttccgc gctggccagc cagggcctga cggatcgaag
     3481 cggactgagg ttctcggggt gggcactgag cggatccgga aggacccgct ggcgggatga
     3541 ctacctcgaa gcgatggacg acccgttggc gacgcgcggc gtgggcgggc gagtccagtt
     3601 cagcgttctg gcgccgttgt cacttacgtt ccgcacgtac ctcggtggcc agcgcacgac
     3661 tcaggcctac atcactcaga ccggcgagta cggggaccac accgacacca tccgcgaggc
     3721 cggcgtagtt ctcgaaggga agctgccccg gatcagcacc aggttgtcgt ggtcaacgga
     3781 gctgaatgca caacgaagga cctcgagcga tccgacgtac gccttcgatc gcgccgcatt
     3841 gacggtgggc gtgaaatacg cgtggtaagc gaaaacctct gacgcgatcc ggccgctacc
     3901 tgaacacctg gaagggcgat tgcgtaatcg ccccgttgat aagcgcgatc gccactcccg
     3961 ccataccgat caacccgact ccgcatgcgt atcctgcgtt cagcacgatg gcgtatttga
     4021 accactgttc cggcccgccg aacttcttcg cgaggtaata ccggctcaac aacgcgcccg
     4081 tgagggacag gaaaatgccg tgcggccacg cgcccagacc accgatcagg ccgtaccacc
     4141 acatcggtga ggcgtgcagg aagtgaataa tcgcataaag gacgccgcca cctatcgccg
     4201 catacccgat cacgtcgaac ttgatggcat tcagcatggt ggagttgccc tgaatcgttg
     4261 ccgtttgcca gagacacgcg ttgtacgcgt tcagcggcca catcttcagt gcgaacggat
     4321 acgccgacga ggggatcggg ttcatcttcc agatcaccga ccagaacgcg aagctcagga
     4381 tgaacgtgat gggaaacatg atgaactcga cggcgaagta gctcgtgaac ttcgtcttcg
     4441 tcagttcgat ttcccggaat ttggacacgc cacccgaaaa gtcgtttgcc tgagaaccca
     4501 cccaccagac ggcgataccc ttgtacccgg aaaggatgaa agccgcctgc gtaatcggcg
     4561 ggaacgccac atgtgtaccg gcgtgcccga tcatgcgcgc gccgatgtag ctgatgaacg
     4621 gcgtgtagac gaacgcgaac aggatgtaga tccacacggg gaaaagcggc accagccagt
     4681 gtgcgaggcc aacgaagaag gcgctcgtta cgaaccagat cgccgccgca agccacaggg
     4741 gaaggtcgcc ccgccccggg ggaggagcga acgtcccggc gccgcgtccg tcccgttgcc
     4801 tcatcaacac cttcgtagac tcgtacaccc cgaggaacat gatcggcagg ccgacgccga
     4861 gcgacaccga ggtccacagg tcgaggttgt tgagaaccga cgcctgcacc acgcccagtc
     4921 cgggccgcca gcttttcagg atacccgctt ccacgaggaa aggaggcagc acgaatgagc
     4981 tgagaaacgc gctgatcacc tgcccgcaga tgatcggaaa cggcacgacg aatccccaca
     5041 tgatgccgcc gaggtcggtg ttgaatccga gcaacgaacc cggcatgacg ttgccgattt
     5101 cgcgggtgaa atccacgaag gggatgggga ggatttcaat cgccgtaccg aagatgcccg
     5161 cggtcaccgt gggtacgccg acgtagatcg cgccccacac gaggccgatc atggagccga
     5221 tgctgaacca ccgccagcgc cacgtttcac cttccgatcc ctctgccagc gccgtaattc
     5281 cctgggcggc gatgggtgcg aacgggaagg gcagacgttc aacgtcgctc gtcagccgga
     5341 acaggaagta gtaggagctg agtgtctgga agcggctgat gagactcgtg aataccatca
     5401 gcgtgacgat gggcagccag tccttgtgcc agaacgtgcg cagtgccacc gcttccgacc
     5461 cggcaggcgg cacggcccac cagatgttct ccgtggtaag ccgttgcgcc acgccaaggc
     5521 tcagcgcctc ctgcgatgac tggaggtagg tgttccagat caggcccccg aacgggcctc
     5581 cgcccaccag cccgccggca aggatgaaca ggatgtagat ttcctgccgc ttcagcggca
     5641 tgaagctgcg tcgggcgagc tccgtgaaca ggatgatgga aacccattcg gccgccgagc
     5701 ccagccccgt gccaactacc agaccgaggt agatggaggc cggcatcatc acgattgcga
     5761 cgaaaagcgc gccgatcaga gtgcgagccg tcagaccact ttcatacggc gccatctcca
     5821 gctcggggtc catgccgtat ttcttgcgct ccgccgcaag cttatcctcc tgggtggagc
     5881 gatcgccctc cgctctaccg cgccctgtca gctttttcca gaaaccccgg tcgttatccg
     5941 cgtcagccat ggcctacctg tcgtcttcct gttgattcct gcacaccgga aaccagacgg
     6001 cgcgcctcac gtcgttttct gagaaatcgc ccgctcaccg ttcacgctgt acatcgcgcg
     6061 gccccgctcg ttgaacatct cccgttccgc cggtgtaagc gtacgccaga tcaggaactg
     6121 cttgcggagg tagttgacga accggcggtt cacacgattc cacgatggga cgtcgcccga
     6181 ttcccgtgtg acgagcaaac tcatcccgaa aagacccttt tcgttcggaa ccggcgaact
     6241 gatgagcgtg acaagctcac ttacgccaag atcgtacggc gcaagccata ccatcgtctc
     6301 gagcatgaac ctgccgctgt ccgcccagaa gtccacgcgg tcgatataga aatcgctcgc
     6361 cgattcatcg cggtgcgatt cgaagaaatg cgcgaggaag gcgttgaccc cctccagttc
     6421 ttcatccatg agcgtgaacg gcagcgcaaa aaaccattca tccccgttcg gttccggcag
     6481 cttccacgtt cgggcgatgt cgggcacgct gatttcagac gccttcttcg ccgggtagat
     6541 cgtggacagg attacggtaa tcatcacgat aagcgtagag acgattgcgg aaagggagga
     6601 gtagttcagc gtcaccccgc ccagaaggcc ggtcttgtag atcacctgcc ccacggtttg
     6661 cccgagcaga tacccggcga tcgcccccag aaccgcgtac accaccgctt ccgccatgaa
     6721 cagcattgca atgtgcgacg gagccagccc tacggagctg aagatcccga tttcccgcgt
     6781 tcgttcgaat accgcgccca gcatcgtgtt caagacaatc agtgcggcga tgagaatcgg
     6841 aatcagtaca tccttcagcc cgccaagggt ggtaagccct attgcgctgt agaggaacgt
     6901 cccctcgttc tggccgacgt acatctccag cgcccacttg ggaatcaccc ggttcaccag
     6961 ccccagcgcg gtgttgctgt ccggggtttt gatggctacc gaaaccagcc cgccgggcag
     7021 gagattcagc ggcatgaaca tgcactgcgc gggcacaagg tgaatgaagg aatcccgctc
     7081 tccggcggtc ggggccttct gcgcctgcac cacctggctg attctatcct gccgctttgt
     7141 ttccaccagc gcccagttga cggaagtcag ctcctcgccg tcaatgtcct tcaactggcg
     7201 gagaagctca tcgtcaacaa tgccaatcac gccgtaccgc accccatcca tgatgatttg
     7261 cggcgccgtg tcctcgtccc ccgtcacctg gtcgcgtgtg atcccgagcg cgtccgcggc
     7321 cgtctgcggt atcaccagca cgcggaaatc gcccggctgg aaccagcgcc ccgccacgcc
     7381 ggttttccgg aaaatcccgg ttacctcatc ctcctgaggc tccatgccta caaatccgct
     7441 cagagtgtag ctcttgccgt tgggtgcgct cagcgtgaac aggttgctcg ttgcaccctg
     7501 aatgagcgct tctcgccatc cacggcccac cacgatgttc tcttggccga attcgttccg
     7561 gatggaggtg agcgtctctc gcggccagaa attccagaat cggtctcgaa tgagaacacc
     7621 gctgtacgtg ggtttctgcg cgctcagccg caccttgttc acccgcagga gctcctgcac
     7681 gctcgtgaat gacagcaccg tgaacgtcag cagtatcagt gtgaggcagg tcagcgtcgt
     7741 ccggaccttg cgttttcgca tgtttgaaac accaagcaga aatgccgcga acatcgcgct
     7801 cacgcgccct acatccgccg aaagataccc ctgctgctcc ttcttgatct gcttcatctg
     7861 cgctgtgaat ttgccgatga tgattgaaac gacaaacaga ctgagcgcga acacggtgaa
     7921 cgcgagaagc accatcggcg gcgccagagt gatgtcgaac gccgggtgaa cctgctggat
     7981 aagcacgaac atcaggatga agatggcgaa gaaccagatc gcctgctttt tcacgtccgt
     8041 ggacccgatg atgaggcgtt ccacgaagaa cgcgaacggc aacagcaacg ccatgtagaa
     8101 cagcacgccc ttcagcgcgc cgtttgcgat acccagtacg tccggcagca cacgcgattc
     8161 cagcgcgagg gcggagcgtg cctcggtgag atacgtgtcg taccgtttct cctccagcgc
     8221 cttctttgcc agcgcgagac gatacgccga tgagtcgtga acggcccggg tctccgcgtc
     8281 gatgattccg tggcggacaa gcgtgacgag ccgcccctcg ttcaactgcc acatgtcccg
     8341 cgccgcctgg taggcagtat gcgtaagcag cgcttcgcgc tcaaccttga agcccagtcc
     8401 ctggcggtgc tcggggatct gtatgctgtc cggggttccc gtcagcatca tccgcacgcc
     8461 cagaagcccc tgcgcgaaga tgattttgac cctggtgccc tcctcgacga aaaatgtgtt
     8521 gtctcccatc gccacgtgag agaagtaatg ccagggcttg gaatccgtgc gcgcctcgta
     8581 aatatctccg cgggccagga actggaaata ccgcgggtcg agtacatccg tcacgctcac
     8641 cgatgcgcac gggaatacga caaccgtcca gtcctgagaa agctgcgaaa cactcggcac
     8701 gaaggcgtac tgcttgctgc caaggtcaat cgcgaaccgg acgttcccgc tatccgccga
     8761 tgtcttgtag gcgtgaagcc attgaggatc tgcggagcgc gctgcggccc tcccgtcgag
     8821 gggcaacccg acgaactcaa atgcgccgcg gcggttggtg atcgtggtga agcgatatcc
     8881 ccctcgatcc gcaatcacga cggcgctgtc taccggctgg tccgccgtca cggaagcctg
     8941 ccggtcgtca tacagcacga ggcccgaaga cgtggcgaag taatcgccca tgaccatgtc
     9001 atcggggagg agcggatcct gaagtgcctg acctgtgatc gcggtcagag ttttcatctg
     9061 cgtcaggctg ccggggatgt tgacccggga aaacgtatca cgcaggctgg atagttcggg
     9121 tgcaatatct ccggtcgtgg aaagagcgat ggcgggaatt cctgccaggc tcgcgatttc
     9181 actcgtgaaa taccgcgctg cggggaggaa attccaccac gctttgcccg gtgtgccggc
     9241 taccccgtcc acgaaccggc gatcgccctg aaggcccaga aaaccgataa actggttcag
     9301 cgtatcaagc tgcccctgtg tcagttgagc ggggggaatt gttcgccacg gtgcgatttt
     9361 ctggaacatg acaagctgct gggcgataat caggctatcc tgccgcagct tgttcagcgt
     9421 ggccggtacc gcgttgatga tattctccgc gaagaactcc ggggcctcca ccatttccac
     9481 gtagcgcttg tgccgctccg ccgccagacg aatcttcgtc ttttcctccc gcggaatctg
     9541 ctccgcgttg atcaggaggt ccgtgtggtt ccagataagc tgatcaatgc cgtgatggat
     9601 cttctccgtg tttttccagt ccattcccgc aatcagcgca tacccggtaa gacccagtcc
     9661 cgccacaatg ccggcgatct gccaccacgc gggtgtgatg ctcgtttcat cagcgcgtct
     9721 gcggcggtac gcaagccacc cgcagacacc cgcgagcagc agaccccacg cccatgcggt
     9781 cgcgttgaca aaccgctggt accagtcgta cgagtagttc acctccgtgt cgctcagcgc
     9841 gattccggca accttccggt aggtctccgc cttcgcgctg atttcggcaa tcttcgccag
     9901 gatcagcgcc cgaatctgct catccgaacg cgaccacaac ccgttcagat cgtttaccac
     9961 gacgaggttc gtggcatacc ccatgaaccg ctttccaagc ggcccgtaat cgcgctgaag
    10021 ttccagatcc ggctgctggt tgtagaagta ccccttgtgg aagaggccga tgttgttcga
    10081 attactcgta agcgacaggt cgataaggag tgccgggtaa cggccgagga tgggttcaat
    10141 aagcgcgaga tcgctgcgaa gccgttccaa tcgcgtggtg tggtcatccg ggctcatccg
    10201 ggtgaacttc tgcagcgcct tcgaaatatc cgtcatccgc gccgcggcaa gcctgccggc
    10261 aacggacgtt cggatctttc cctcatctgc atccacgatc ggtttgaggc gcgcccggtc
    10321 attctccagc tccccgacga aatcctcgag aaggcggccg tagtccgcga taagcggctc
    10381 acacgcgtcc agcgcccgct tgaaggccgg tttgaacctc tcagccgcct cctccatggc
    10441 tccgacacgc acccgccggg cataatagag agctccgccc gtacgttttt cggcaaccgc
    10501 gccgcccttc agatactccg tccgggcgtt ctgaaggtca tcccagtagc gttgtgccgt
    10561 tgccagttcg ttcccgacca catctcccaa ccgccgctca agcgccagtg cccgctcgcg
    10621 atgagcagga agccggccgg atagcttgcg gtcgctgatc agtctgagat ccgcacgcaa
    10681 tctctccaga ttctgccgca gcgcccccgg gtacgattgc ttcaacgcct gaacgaaaac
    10741 gcgtcccccc tccatcacac cgcttcgttg gccattgagc gccgcaaaat agagcgaccg
    10801 tggtggccgc tccttcccga aaatccgcgc aagctcaagc tgtgccgcgc tgctgaacgc
    10861 cgcctcggcg ccaggggcca gacccggaac attcgatcct gcgtcaaacg gcgcggtgat
    10921 aatcacgact tcccgcgaaa gaaccgggtc agagcccggc agaaaccccg tcacgctgtt
    10981 accggtcagc atacgccacg gctgtttcgc gtgtatcgtg atctccgtgt tggggtgcgc
    11041 tgccgcccac gcccgcaacg aatccccgtc ctgccggttt acccagaaac gagggaagtt
    11101 cacggaggtg tagaactgcg ctgatatggt taccttgggt gcttcaatga aaatggccgc
    11161 tttgcttcca agcgtgctcg caagcctcca gcgttcttcc accgtcatct cggcgagaag
    11221 aacacttcct tccatctcgt ggccattgta ttcccgcgcc tccccggtcc ggctgtacac
    11281 gaggtggcct ttcaatcctc cgggtggaat ggtgttcgtg tgggcgaaat tcggcgcgta
    11341 acagcgcagt ggaatcgtat cgaaggtgcc gtcctcaagc ggaagaactg catacgcata
    11401 ttgctgccgc gcatccgcaa ccgtaacccg ctgtcgggtc acgtgttgca tgccaatgct
    11461 atcaaacgtg gcggcaatgt agtccgtcgc ggcttcgttc ccggccgtgc cgatctcccg
    11521 cgtcggataa cgcgagagca gctcccacga acgcacgaag cgcgcggaat cgatctgcgc
    11581 ggcgtgccgc gaaccgatct ctccgagctc cactgcgctt gggtcgcgac ggtactgcgc
    11641 gccggcagtt gagacagcgc ccaggagaca cgcaacgaaa aacgtgatct ttgctgcccc
    11701 gcggacgccg tacgcagtcc cacggagaaa cggcagtctg atacgagtca tggttgatcc
    11761 gaggattaac gtggcaaaac agcggtgaga tcgatgcatc ccgattccga atctgagaaa
    11821 ataacaagac cgcggcactg cgtcgagcct ccgttttcaa acaaacgcct tttttcgcgc
    11881 actcccgcga acgatcttat ggagggcaca cacacaggag gaacttccat ggcagaaaaa
    11941 acgctcaaag gcagaaccgc actcatcacg ggaagcacca gcaccggaat gggtcgcagc
    12001 atcgccctcc gcctcgcggc cgaaggcgcg aacatcgtcc tgaactacgg cactcaccgg
    12061 gatttctcca cccacgacgg tggcgcggat gctcaggccc gcgcggagga agtggcctcc
    12121 cacattcgcc gcctcggagg acaggcaatc atcgcgcccg gtgacacccg aagcgaagag
    12181 gacaccatcc gcatggttga cgcggcaaaa aaagccttcg gcgcaatcga tatccttgtc
    12241 cttgcagcag gcggcaagtg gaaacctcag ccgctcgagt ccgtcaccat tgatttctgg
    12301 cgcgatgtta tggcggccga ggttgacgcg atgtttctcg cgttcaaaca cgtgattccc
    12361 ggaatgcgcg cgcggaaatt cggacgcatc atcgtattca gcatgaacgg cgccatgacg
    12421 cggaaaaccc tcgaagcgac ggccgcggac tacacgatcg ggaaaacggc ccgcacctgg
    12481 ctcagcctcg cagtcggtca tgacgaattc gaaaacggaa tcaccgtcaa cattatcgaa
    12541 cccggccccg tcggtcacat gacattgaac gaggcacttg ccgccatcga gcacacagac
    12601 aacgcatggg aacaacgcgc aaagccggtt gcgcacgatg cggccgaact ggctgccttc
    12661 ctctgctccg aagcgggccg attcgtcagt cagagcctga tccgctatcc gaccgacgga
    12721 tggtgagaag aacacgcctg cgataaacca gggacaccaa ccacgacgtg gcgcgacttc
    12781 tcccggcgtt gcgccacgtc tgttatgtta tccgtagcta tcgggttttg tccgtattct
    12841 gcctcggaaa ggagcacctc gcctgacaat cacccggttc tggcttaaca ttcgatacag
    12901 tgggatacaa cgacatcagt gcgatcacgg cctgtggtcg ccgtgatcgt cctcctctgt
    12961 ggccttgccg aaatacctgc cgcaccgtgc gaagcccttt acttttgccc ctttacgtcc
    13021 cggcgtgaag cgaatactgg attatcagtg ttatgtaaac cgcggagtcg cagcctgtcc
    13081 ccctcttggt acagcagaga aacaacttgc ccgttgccct gcatggcccg ccggacaagc
    13141 aaccccttga ctgcttccgc ggtagcgcgg tacaatcgga cgacaattcc cacgggaaat
    13201 cagtgctgaa tcacgagcgc caccgggaaa tcatgcgcgt ccgaacctcc ccaccaagcg
    13261 gccagcaggt cggtgaaatc ccgtttcctg ccggcagtga tccgtacacg ccagtagatc
    13321 gtttccgcgc acagggaccc acgccatgcc gccaaaactg aaccgaagcc cgattctgtg
    13381 tttctcccac atcaaaggcg gagtcgcaaa aacgacgtgc tgcgtcaatg tcggggctgg
    13441 ccttgccgat atggggtatc gaaccctgct cgttgacatg gatccgcaaa gctcgctcac
    13501 cgatgtcttt ctgggaaacg gcgaacctcc ggcggcttcg ttgtaccagg tgctgtacgc
    13561 cgatgggccg ggccagcggc agatggcgat cagcgatgtc atccggcctg ttcgagaaaa
    13621 tctcgatatc gcgcccgcat cgctggatct cgtgcgcctc gagcaccttc tcctgccgga
    13681 agtggacggg cgcgacaggc tccgccgtgc actgcggggc cacggctacg actttgtcat
    13741 catcgacacg agcccctcgg taggtatctt gacttcgaac gcgatgattg ccgcaacgga
    13801 cctcattatt ccggttcaag cgagttactt cgccctcaaa ggcatcgaga acatcatgga
    13861 gacgttcaat accatcaggg agcgggtgaa cgagtcgctc aacctcctcg gcgtcgtcat
    13921 caccatggtg gacaagagaa aacgccaccc gagggaagta atcgccgacc tggtggagat
    13981 attcccgcgc gaactgattt tcgacacaca gatccgggaa gtcgccaaag ttgaggcgag
    14041 ccccggctac caccaggata tcttctcctt cgccccggaa agccccggcg cggacgattt
    14101 ccgcgcgttc atccaggaga ttctgacgcg atgccgaaat tcgtgacgaa aaccatctcc
    14161 cttgggcgca ccggcgataa cgaactccgg aacctcatcg cgcccgacaa acgtatcggc
    14221 aaactggtgc ctgtagacga gatcacgccg aatcccgacc agccccgcag gcatttcgat
    14281 ccggacgcac tcgaagcgtt gaaggaaagt atacgccagc acgggctgat caacccgatc
    14341 accgtggacg aaaacggcag aatacttgcc ggggagcgcc gctaccgggc gtgtgtcgca
    14401 ctaggtatgc gcgaggtgcc ggtcatccgc atgagcggct cgctggaagt ctccctggtg
    14461 gaaaacctgc agcggcaaga tctgcacccg ctcgaggaag cgcgaggata ccagactctg
    14521 gtggacgcgg ggtacaccca cgagcagatc gccacgcgca tcgggaagga caggtcggtg
    14581 gtcacccact cccttcgcct gctcgacctc ccggcggata ttcaggccga atgtgtgacg
    14641 tcacacaacg ttactaagga tcaattatta cagatacttg gcgcggatag cccggagcag
    14701 cagagaagaa tatgggagga catcaaagcg gggaagtccg cccgtgctct caagcaggaa
    14761 cgcagtgagg caaaacgccg ccggcagatc acgcctcgtg tgttcatcaa ccgtgtgaga
    14821 agcttcagca ccgacgcggg aaaaatcgat gtcgcccggc tgagcgatac cgagaagcgt
    14881 gccctgaaaa cgcacatcga acacgccctt gcgcgtcttt cgtcccttct cggcgagctc
    14941 tgaggcccac ccctccctgg cgagcccccc ccacgcgcag gacacccatt gactgcgccg
    15001 ccacgcggac ctggagttcc ctgaagcgcg attcagggaa ctccaaaaga actcgacccg
    15061 cccgcgcgag ccgagttcta atcgttgcgt gcgtacaggt gcccctgagg gtcccccagc
    15121 agaagttcgt gcgtatccat gagcagcgcc gggatatcaa gcttttccgg gcacctgggc
    15181 aggcactcgc cgcatttcgt gcaggcggtt gcctgttctc ccggaaacca gtgtccgccc
    15241 cgactgaaca ggttgtagcg gtatttcccg aactccttca tgtcgtacgc tctggcgagg
    15301 ttgcgcaacc gcagcgtctc cggaatatga atattctccg ggcagggcag acactcgtag
    15361 cactgggtgc agaacgtggt cccgagtgct cgcctcattt cctcgtccag ccgggcgaac
    15421 agcgcggctt cttcctcggt ccacggccca tcgttgtccg caacggcaag atgctcttcg
    15481 aactcgtgcg gagaggccgc cccgaggctc agcgtgtgaa tctccggatt gcccagcagg
    15541 aaccggtgat tggcctggat tggcgtgtag ggctttgtca actcccggag tttcgccgga
    15601 gcgttgaaaa gctgccctcc cttgtccgtc ggagaaatga tgaacacgcc catatcgagc
    15661 gaccgggcct tctcgattgc cgggagattg cgccggttga acgtgtagta gtgcaggttg
    15721 accgactcga actcccccgt gccgattgcc tccatgataa cttccagcgg cgcgtgtgtc
    15781 gaaaaaccga ggtgcccgac gatcccttcc tccatcgcct tccggattgc cgcgagacag
    15841 ccgcccttct tcaacgtgcg ccgaagaatc tcgcgattgt tgatgccgtg gatatcgaaa
    15901 ttgtcgattc gatccacgcg catccgttcg agcgattcgt ccagcgaccg gcgcatctcg
    15961 tcagccgtat cggcggggcc tattttggtc gtgatgataa gcttttcgcg cggcaatgag
    16021 ggcatcgcaa gacccatcaa gcgctcactc gacccgtacc ctctggccgt ttcgaggtgg
    16081 ttgatcccca gctccaccgc gcggcggatc gttgcaaccg cattctcgtc cgtgccgtcg
    16141 gggatacgca tcgcgccgaa cgtgaacacc gaaaccatga gctccgtttt cccgaatcgc
    16201 cggtatttca tcacgtcacc tcacctgttg ggctccgccg gaacacacac ctgccccgtt
    16261 aatgtttgtg tcatgaaatg acgtcatcgt gtggacttga ccaaagtgcc ggtgacgcgg
    16321 tatcaatcaa tttcgagcgc gtgcccgttt tgcactcgca ccatacgggc ccacgaaccc
    16381 agttcctgtc cgtgggacgg gagaaaccga agtatgcagc cggtacaggt gtcgtcgcca
    16441 gtcgcgggcg atgtcggtcg caaccgcctc gcgcggcgca ttctcaacaa cgccctcgcg
    16501 tcaataatcg aaccggtgcg tccccatttc gactccgttc tccgtcagct ggactcaatt
    16561 cttactgcgg aagtcccgac cgtcgacgcc gtaagtcgct acgtcgccgc cacgaagggc
    16621 aaatgtatca gacccgcgct ggtgctgctg gcagccaggt gcgtgggggc aattacgcat
    16681 gaagtccgtc tcgccgcggt cagcatggag ctcctccagg cctccactct gttgcatgat
    16741 gacgtgatag atcagtcggt cctccggcgc ggtgtgccct cggtaaacgc ccgctgggat
    16801 aatgaaactg ccgtcttgat gggtgacatc ctgttcacgc aggcgctctc caccatgctc
    16861 gaaaccggtt cgatccccgt gatgaaaatc gcggcgctcc agacccgaag aatgatcgaa
    16921 ggagaggtcc acgcccgcga caagagacgc tctccggtct tttccgagga gagctacctc
    16981 gacctgatca atcggaaaac cgcgtctctc atgtcgttgg ccactgaggg gggcgcgcgt
    17041 ctttctgacg gcaccgaaca acaggtgaac gcgctcgcat cgtacgggga gctcctcggc
    17101 atggccttcc agatcgtgga tgacatcctc gacgtcgtgg gcgatcccac cgtcgtaggc
    17161 aaacccacag ggcaggacct ccgcgaagga accatcaccc tgccgctcat ccgtgcattg
    17221 aatgccgccc cgcacgagga tgcacagaaa atccgagagc tcgtttccct cggggtgaac
    17281 acggatgccg actggaacgc agtccacgcc ttcattgagt tgtacggagg cgtcgcctcg
    17341 gcgcttcgca ccgcccggca ctacgcggaa caggcgcgcg cgcacctcgg catcctcccg
    17401 ccatccgatg ctcgcgattc cctcgaccgg atggtggggt atgtcataag ccgcgaaata
    17461 tagaagctcc cgttctgagg tccccatggc acggatttcc atccgttcaa ttcgttaccg
    17521 ccgtccgctc aatcccacgt gggataccga actcgagcag cagattcact gccaaatcga
    17581 gggcatcgaa gccccgctgg acctggccgg gctctttcag cggcaggcgc ccgtgcacgt
    17641 cgaaatcggt ttcggcaagg gccggttcct gctcgccgcg gcagaacgat tccccgaaac
    17701 gaactggctg gggatcgaat acgccccgcc gtgcgtcgcg cttgcggtgg aacgcgcagc
    17761 cagaaaaggc ctccgtaacg tccgaatcgc ccgcggtgtg gcggaggaca tcattgctgc
    17821 gcgcttcccc gcggacaccg ttgccgccta ccatatctac ttcccggacc cgtggccaaa
    17881 aaagcgccat cataagcggc gcctcatcaa acctccgttt gccgtccaac tcgcccgaac
    17941 gctggagccc ggagggtttg tcaatacggc aacagacaac agcgagtatt tcgaggaaat
    18001 catcgctgcg tgcaccggtg ccggacttgc acgcatggac gtgccggaca tctacgggga
    18061 cgaagccttc cgcacaaact tcgagatgcg attcctcacc cacggccaac cggtctaccg
    18121 tgccgcgttt cgcaaacggg accggtaacc tgttctcttc tcccaattcg gcggaatctt
    18181 ctcctcgttc cctgcgtttt gtaccttcga cgaacgatcc cgtacacgtc ttgcataagg
    18241 gaggaacagc catgccgaca aaaggggaca acgcaccgga cttcagtctc gctgatcaga
    18301 acggggtagt acacacgttg aagcagtacg ccggcaaatg ggtcgtgctc tacttctacc
    18361 cgaaagacga cacgcccggt tgcacgaaag aagcgtgcag cttccgcgat aacctgggcg
    18421 ccgtcacgaa aaagggcgca gtgatcctcg gcgtcagcgg cgattccacg gcgtcgcaca
    18481 agaaattcgc cacgaaatac gacctgccgt tcccgcttct cgccgacacg gaaaaagcgg
    18541 ttatcaacgc gtacggcgtc tggaaagaga agaacatgta cggcaagaag accatgggcg
    18601 tcgagcgctc caccttcctc attgaccccg ccggcaaaat cgccgcggag tggcgcaagg
    18661 tgaaggttga cggccacacg gaagaggtcc ttgccgccct gccgtaaccg cgagccaggg
    18721 atcctcccac gcacctgaat tgaggtctga ggggcggttt ccctgcctgc ccggattctt
    18781 cataggcccg gcctcgtagg gggcgggttg cccgcccccc cttttttccg tttttgcgcg
    18841 cgcacggtca tcgagtgact tctggagaac gaagcgaagc gccgttcccc gaacgttgta
    18901 tcgagatgac cacgtccgcg gggcccccgc tccccccttt cccgccgttt ttcccgtgtc
    18961 ctaaattttc acacagaccc ggtgttcccc gaatctctca cggcggcgcc catggaactc
    19021 ttccggaccg cgttccttgt tctcctcgcc ctcggtgcaa cgggctatgc cgttgccagc
    19081 gtcgcgctgt ggactggcgc gcttcgccgg tctcggcgca tccctcgtgg tggcgacctg
    19141 ccaaccgtca ccgtcatcgt gccggcgcgg aacgaggccg cgaacatcgc ctcgtgcctg
    19201 accgctctct cacgccagtc ctatccccct cacctctgca agatcgttgt cgtggatgac
    19261 cgctccgacg acggcaccgg cgaaatcgcg gcgagggcgg cctgcgcgat ggaggctgtc
    19321 gtgcaggtcg tcagagtgga taccgtgccg ccgggttggt cgcccaaaaa gcacgctctg
    19381 caccgggcca ttctcgaaag cacctccgac atcatcctta ccactgacgc cgactgcgag
    19441 ccggaagagt gctggatcga ggaaatggtc gcgagtcttt ccgcggcaca tgtggtcgca
    19501 ggctactccc cgtacaggga ccgcacctcc cttccggccc gtttgctcgc tctggaaaca
    19561 ctctcccagg ccttcctcgc catgggcggc atcggaatcg gcagacccat cacctgcacc
    19621 ggcagaagcc tcggctaccg ccgcgaggtc tacgccgccg tggggggatt cggcgaagcg
    19681 cattccatgc tcagtggcga cgaccacctc ttcctgcaac gcgcggtcgg ggccggtttc
    19741 acggccgcgt tctgcgatgg cccgggcagc tacgtctgga cgaatccgcc gccgacgtgg
    19801 cgcgggttcg tccagcagcg aatccgcatg ttctcgggcg cgcgcatgtt gaagcctgcc
    19861 gtcgcagcgc tgggagtttt cgtgtatgcg tggctggcag tgatgggtgt cggaatgctt
    19921 gccgcctttc cggcagcatg gggcgcgttc ggggtgaaag tgctgctcga cggcgtcagt
    19981 atgggaattg ccgcagttcg tctgcacgag aaaaggctgc tggcggtgta cccgattgcc
    20041 gcgtttctct acctgcccta tttcctcgta tttgccctgt ggggtacggt cggctcatac
    20101 cgatggaaag gtgcccgcgg gcactaaccc ccctgttccg gcttcttccg cggcgcacgc
    20161 ggagttttct ttctggcggg cgctttcggc ttcttcggtg cctccgcagc ccccggctgc
    20221 agaacccgtt cttccgtttc tttttgcgcc ttcgcaggcc gctttgctct cgacggttgt
    20281 ttcgttggcg cggcgggttc cttcagaggt tcaggtgtca cttcctccac ccgctgcggc
    20341 gccttttcgg gttgtacggt gaccggggtt tcttccacat cgggcctacc cgttgtttct
    20401 gccgctgccc gggctcctcg tcggcgttcg tgctcgggag gtacgttcac cgcagcaacc
    20461 cgccggtcag gcaatgcccc tatccggtgc cccaccgcgt atgccttccc gagcactttt
    20521 tccgccgccc gcgtcttcac cggccgcttt ctgcacccgg cgagaacggc cagcgcggct
    20581 tcttcatctt tcacaacggg aatcagccgg tcgagtagcg tctcttcgaa aaactcttcc
    20641 agatcatcgc tgcagtggaa cgcgaacatc agcccgtacg agggcgagtg aagtttgtac
    20701 gccgcggcca gcacatgcag gatttcccgg tccgcgaact tcacccccga aaagtcaatc
    20761 gcgatcccct cataaccgtc gcgaacgcct ttctcaagcg cagccctgaa ctcccgcgcc
    20821 ccgtgtgtgg ccgtcagcct cgcaaatcgc ggatgaacga catacacgcc gttcgcctct
    20881 ttcacatgca cactcatgtt cctgcccccc gaaagattcc tgccgacact cgtcagcccg
    20941 gcttcacagg tttcgctttt tccctttcaa agcctcaatc gctgcgtcct cgtctgatac
    21001 gatctctatc acatcatcca gcacggtgtt tttcagaacc tcgtggaccg catcacccgc
    21061 gccgaacacg cacagacgac ccccatttcg atggataccc ttgaacgcgt caatcagaac
    21121 ccccatcatg gcgccgtcca catattccac acccgaaaaa tccacggcaa cggccttctt
    21181 tccctgctct accgcctcct caagacgtgt gcggacggcc ggcatccccc ggtgcgcggt
    21241 aagacgcttt tgatccggtg tgatgactac taccccgcgt gtgatccgga cggtggcaga
    21301 catggctcga ccccttcatg cggaacagac aaaggcctgt ttggtgcaga caaccattca
    21361 acttagatgc agcggaaccg tgggtcaagg aatcccgttc gcggcgaccc taacacaaaa
    21421 ccacacgggc ggttacggtt gcggtttgct ttccggcgct ggttttccta ttttcttgcc
    21481 cagacagaat ttcgcatgcc gtcggcagaa acaccacctg atcgcggggg attccagcca
    21541 tgcaagtcac catctgggga tgccggggat cattgccgac acccatgcca aaagcggaat
    21601 acgaaaagaa actcagcgac atcctcaccg cgtatcggga cgccggagcc cccgccgaca
    21661 tcgaggcatt cctccagagc cgcccgttcg tggagcgttc cacgttcggc gggaacaccg
    21721 cctgcgtcga agttactgaa ggcgaccacc agctcattct ggacgcaggc agcggcctcc
    21781 gacccctcgg cctcaaaatg atgggtggcc cctgtgggaa ggcaaagggc gatgtgcata
    21841 tgctcatcag ccacacccac tgggatcaca tcatgggctt ccctttcttc gtgccggcct
    21901 acatcccggg gaacaaaatc accatctacg gctgccacga taatctcaag gaacggttcg
    21961 agtaccagca ggtgttcacc catttcccgg tcttgctcga tcagatggcc tcaaccaaaa
    22021 cgttcgtaca ggtagaggtc gggaagccgt ttgagatcgg gccgttcaag atctccacaa
    22081 tcgcccattt ccaccccggc gacgcctacg ggtaccgcat cgaaacctgc aaagcgtctt
    22141 tcgtgtacgc cacggacgcc gagtacaccg aagtcacgtg ggaacaacag cagaaacaca
    22201 tcgagttctt cagaggtgcg ggtgccatcg tgttcgacgc cgcatacagc ctgaaagagg
    22261 cggtcgaaaa gctcaactgg ggccacggat ccccgctcct tggcgtcgaa atggcggtgc
    22321 aggccggcgc caagcgcctc atcctgttcc accacgaccc ggcaaccccg gaaaaacaga
    22381 tcgccgaagc acatgccaaa gcaaaacggt tctacgacga actgcccaac aaaaccacgg
    22441 aatgcgaaat cctcaccgcg cacgattaca tgaccctcga cctcggttga ggtcgctcac
    22501 ccctgcgtga gcccgctcga ccttggttga gcccgctcga cctcggttga gcctgcttac
    22561 gaccgcgtga ggctgctttc aggcggggag caaagtccgg tgctccccgc ttctttttgc
    22621 gtccgtcttg accgcttcct tcgcattccc tacgttgtct ttcccttgct tccatttctc
    22681 atcttctgcg cggagagaac ttgtatggca gatactcgtc gtttttccaa tcctgcagcc
    22741 gatgcctgga ttgacaaacc catcccctgc ctcgaccacg gcttcgttat gctcaaggac
    22801 tacatgggag gcgacgcctc aatcgttcag gcagcccgcg tcagttacgg cgagggcacg
    22861 aaaaaagcct ccgatgacaa gggcctcatc gcctacctca tgcgccaccg ccataccacg
    22921 ccgttcgaga tggtggacct gaagttcctc gtcaaactgc ccattttcgt cgcccggcag
    22981 tggatccgtc accgtacggc cagtgtcaac gaactctccg cgcggtacag cgtcatggcg
    23041 gacgagtttt acctgccgaa aaaggaggat gtgcgctacc agtcaaccct gaaccggcag
    23101 ggaggcagcg acgaggaggt ttcagatgag ctctgcggcc gggtggtgga aaccctccgc
    23161 gccggcgcgg aacgtgccta caacgagtac gaggagatga tcgaagccaa catcgcccgc
    23221 gaactcgccc gcactcacct gcccgtatcg ctttacaccc agtggtactg gaaaatcaac
    23281 ctgcacaacc tgctgcactt cctcgaactc cgcctcgata cccacgccca gtacgaaatc
    23341 cgtgtctacg cgcaggcaat gtcgcaaatc gtgaaggacg tcgtcccgtg gacatgggaa
    23401 gcctttgagg agttccgcct caacgcgcag accttctccg cctcggaaaa agcggtgctt
    23461 gccatgctgc tgcaaggcaa ggaggtggtg ttgcccgaat ccctccagaa aggcggccga
    23521 cggcgggaat tcgaggaaaa gctcgccgcc cttggcgtgg acccggcaac ggtgttgcct
    23581 cctgcgaagt agaggcactc ggttcactcc ggtcgtcgac tggcggcgcg gcgcgcgctc
    23641 cgagtgacac gatggagatc cgcgacgccg tgttgtatcg aggagccgcc cgtaacacct
    23701 gatggaggga cacgctccgt cgtgtccttt gcgctccggt tccagcgcct tcggacggat
    23761 cccgcgattc aatcacttcg agcgcaacaa tctgcagttg cagcgatctc ccgaagccgt
    23821 cattcccgcg caggcgggaa tccatggtgc ggaccccaaa aagttgttac cacctgcgac
    23881 ccaggcccac acccgatgga ggggcacgct tcgtcgtgcc tggacccgac ggagcgggtc
    23941 cctccacctc cgatccagag gaggctgtac gggcggcgca tggtagcggc cgttgccttt
    24001 tggcttcccg ccgtgttgac tcggcggaag tgctgccact gacgcagtga acgttgcggc
    24061 cacccagggg caaacgcagc aacggttgtg tatgtcctcc acgtcccata ggacccctac
    24121 acctcccgcg ttgccaccgg cgcatgaata cgctaccgtt tctctccaca acgcgtgaac
    24181 cgcatcgcac actcacctcc catccgggta aatgaggtat cggaatgttc taccgcaaag
    24241 acggcacgaa agtgttcccc atcggcatgt acagcttccc cgcgaatgac tccgaatgga
    24301 cactatggcg cgaggcaggg gtcaatctcc tgcgctgcgg caaccgcgac cagctcgacg
    24361 aggcatggtc gcacggcatg ttcggctggg tctccgtgcc gatggtgctc gctcccggtg
    24421 acgatgggac tgtgctcgcc acgctcatcg attcgctcaa ggaccacccg gcgcttgcgg
    24481 tctgggaagc gcccgacgaa gcgatctgga acgcgtggta cgacggccgc attccccgcc
    24541 gcctgtggac cgactcaccc gaagaaatcg ccgaaaagaa acggaagatg gacgatctcg
    24601 tcgacgggct tacccgcggc gccgcgatcg tgcgcgagcg ggaccccgga cgcagcatct
    24661 ggctcaatga agccgtgctc tcgccgctcg atatcgttgc ccggtgcgcc gggtcactgg
    24721 acatcatcgg attcgactac tacgcgtccg gcattcccga ggacaacttc gcgtggccga
    24781 tgcagctcat ggggcgggat atcgaccggt tccgcgccgc cgctcccaac tgcgccgtct
    24841 ggatgatcga gcaggcgttc agctaccccg agctgcttcc gcagtattgc agccattttc
    24901 ccggcggcac gcccagccgc gacgaactcc gcttcatggc gtggcaggca attcatcacg
    24961 gcacgaccgg gctgttctgg tacggattgc acaaggtcca gcgccccacg ccattcatgg
    25021 acgacctcat ggcagtcgtt tccgaactcc gccagacggt ggctttcgtc cagagcggac
    25081 cggtccccgg catcactgcg gaggcgcacc attcgtggcg gccctcaatc atgggctgct
    25141 cgtgcaccgt gtggcgtctc ggcaaccgcg tgctggtctt catgatcaac gaggacgcgc
    25201 acagtgtgga catgcacatc cgcgggctcg acgatgtggt gcggggcgga ctcgtgccgg
    25261 tgaatgagcc gtcagccgac ctcacgccgc tggacggggg gttcatcacc ccgatgcgtg
    25321 gccacgaagt gcggctttac ctcgccggat gacgcggata cacgcctgac cggcgcaaat
    25381 gaccgggatg cgcgcaggcg ggaatcca
//
LOCUS       DAOSOO010000021        25096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_1989_length_25096_cov_5.756759, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000021 DAOSOO010000000
VERSION     DAOSOO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25096
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_25096_cov_5.756759"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLT86_02850"
     CDS             complement(<1..865)
                     /locus_tag="PLT86_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOT35585.1"
                     /translation="MANRRSVSGDVRPSGVRLREALVEGLLSTGICVRDLGVVPTPAL
                     YFGILHLKTGGGVQITGSHNPPEFNGFKMCHGLSAIYGEQIQGLRRLIETSDFERGTG
                     QYSEEDIRSAYTSDILQRTAISRRLRVVIDAGNGCASEIAPAIFRSLGCEVTPLFCEV
                     DGTFPNHLPDPTMARYMHDLISKVKETGADIGIGYDGDADRIGVIDDKGRIVEGDRLL
                     ALFARDVLTRVPGAEIIFDVKCSQALPEDIARHGGKPVMWKTGHSLIKAKMKETGTPL
                     AGEMSGHIFFSE"
     gene            complement(1001..1747)
                     /locus_tag="PLT86_02855"
     CDS             complement(1001..1747)
                     /locus_tag="PLT86_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT35586.1"
                     /translation="MAKTLRIAGIQMAVSADLDTNLPKILHNLSIAAEEGAQFVLFPE
                     MSLSGYHGNYDFEKVKGAVETIRTALRKLRVGAIISTGWREGGEAFIQQRAYDECGDL
                     LGTHEKMIPTGIDGESGDRAFCVPGKELRTFQWNGITCGMLICNDMWATPGCGPHKDL
                     RLMYQLGQKGTRIVFHSINSGAGARHTPYHESNISLRAEESHMYVCTANAARPDRGIN
                     CSSGVVAPDGQWVTRVHRVGEGRYHAVLIL"
     gene            complement(1867..2361)
                     /locus_tag="PLT86_02860"
     CDS             complement(1867..2361)
                     /locus_tag="PLT86_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT35587.1"
                     /translation="MNPTRFLEHVDRTYKPAPALLSLIPVERLDWAPATNMMTIRQLI
                     HHLSARTGVHGLVTGDFDPSRCWGDNDDPASITPGMAKERLTKAVEEVKRSLSEVSIE
                     DWEHRIVEMPWGVKGTLETMAYGLIIEHFLNHKMQLFLYLKLLGIPVDTGTLYFGLLP
                     GQKH"
     gene            2511..3152
                     /locus_tag="PLT86_02865"
     CDS             2511..3152
                     /locus_tag="PLT86_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT35588.1"
                     /translation="MGEFTFWLHQLAAHHPAEAYAILFLSAVVENLFPPVPGDTVTVF
                     GAYLVGRGSLELWPVIWATVAGSASGFLGLYVLGRTYGRGVLSRLRWVQRSMPRIALA
                     ERLCRRRGILVVLANRFLPGLRSVVSITVGLLRMPAWQVVPAAVFSIFAWNGLLIWGG
                     LLAGENWERAVEIVSRYNTYFGALLVVMALGVSAWWWGTRRHRRPILHGENPR"
     gene            3149..4600
                     /gene="cysS"
                     /locus_tag="PLT86_02870"
     CDS             3149..4600
                     /gene="cysS"
                     /locus_tag="PLT86_02870"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT35589.1"
                     /translation="MSLQVYNSLTRTKEIFEPIVPGRVGIYVCGPTVYGDSHLGHAKA
                     YISFDVIVRYLRFLGYKVRYVQNITDVGHMTDNDEDQGEDKIAKKAALERIEPMEVVT
                     HYMMSYFDDMAALNVARPDIICLASGHIPEQIALIQRLLDAGVAYVSNGSVYFSVDQF
                     RSYGKLSGRRVEELEEGARVAVNLEKKNPADFALWKKAEPGHLMVWDSPWGKGFPGWH
                     LECSAMSMKYLGETFDIHGGGLENQFPHHECEIAQSEAATGKPFVRYWLHNNMLTING
                     QKMGKSLGNFVTIKEMLRKISPEALRLFILMSHYRSVTDFSDEALNSAEKGMARLSAT
                     VQALSERLPGAPEGAADPETDAELAEIRRKFVVAMDDDFNTAEAIAQLFSVVRLTNSV
                     LSAPNPATRGTLELLNTQFDELGEGVLGLRLKARAEGSEDLTDQIMQVLIQIREKLRK
                     EKQYAMADEIRQMLAEVGIVLKDEKGRTTWARS"
     gene            complement(4610..5533)
                     /locus_tag="PLT86_02875"
     CDS             complement(4610..5533)
                     /locus_tag="PLT86_02875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT35590.1"
                     /translation="MGLRRLTIMIVPHSHQRVREITVTDRALLVGGLILVAIVLLTLT
                     YAIGFHIRTSQAKAVERLKQENAQLASRLQDMSSGVVELKAEVSNLVRKEEMLRVMAN
                     LPEVDSDVRAAGIGSLDVDEDLFSSDDVVTEAGRLGMEVHSDIQSLLNQAKFQRESFR
                     EIERALANNIEFRDHLPSIPPVDLAQVYVSSVFGYRADPYTGRRRIHKGIDLAGRTGT
                     SVVSTADGVVADVSWGGYTGLVVQIDHGYGYSTVYGHLAKALVREGQRVARGQTIAEL
                     GNTGRSTGPHLHYSVFYHGRAVDPANFFYVQ"
     gene            complement(6070..6516)
                     /locus_tag="PLT86_02880"
     CDS             complement(6070..6516)
                     /locus_tag="PLT86_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOT35591.1"
                     /translation="MLKRLLETDRNDLAGFVARVFLGIVMFPHGAQKMLGWFGGHGFS
                     ATMGFFGSMGIPPVFAFLAIFAEFFGALGVIFGFLTRVAAFGIGMVMLVAMTAVHLPN
                     GFFAQQNGIEFTLILFGVGLAVMIKGGGRWSLDELLMRVLSNRTQG"
     gene            complement(6622..7512)
                     /locus_tag="PLT86_02885"
     CDS             complement(6622..7512)
                     /locus_tag="PLT86_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOT35592.1"
                     /translation="MRVACIINPRAGRRPHAEHVIAGLRSVASMHGWELRTVTTTGPG
                     EATQIARSLVPASDIIVAAGGDGTVHEVSAALVGTQVALAVIPCGSGNGFARSFKIPL
                     DPLRAIRSLVDSEQISVDVGTVEGRHFFATAGIGLDAEISRRFAAGNHRRGIWPYFRH
                     GFAAWVAYCPQKVTIQSGNMSLSAKPLVLAVANTEQYGGGAKIAPGASPFDGLLNITI
                     VETSSFLRLLIAFPRLFTGSLDRSPLVRRMTATEFTIHRQDPGPYHADGEPFDGPAVL
                     HFSVVPHALRLLVPRGKYPG"
     gene            complement(7517..8551)
                     /locus_tag="PLT86_02890"
     CDS             complement(7517..8551)
                     /locus_tag="PLT86_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35593.1"
                     /translation="MRRTTPPFRVGIIGCGGISHAHALGFGNLPALFEVRAVCDSNEK
                     AARKLAEPFGEPRICSDWREVIADPEIDAVDILLPHHLHALVGVSALRAGKHVCLEKP
                     LAITLSEGIELVLEAEASNAVFMVAFNERHRWSVRTIKKIVDEGRIGDVYMVRTDHNQ
                     NPRFAANAWYRNGQLAGGGALIGSGIHMLDLLQWFCGTATSVFATGVKLPEVLETETA
                     ASVIVEFAPRKEGCPDCIGTLDISWAAPNHPWYQFLVVYGTRGTVSTLRGDVVSLRTA
                     GEQEEFHAPEGDTWTDSFREELRHWGECIAQGTRPLTDAREAFRSLEICMAAYRSMDT
                     HTAVRIPLPE"
     gene            complement(8637..9692)
                     /locus_tag="PLT86_02895"
     CDS             complement(8637..9692)
                     /locus_tag="PLT86_02895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT35594.1"
                     /translation="MLDYLQEQTARINQQLEAVLPRSITAEWVEAVLGRSTYSVDEET
                     LTAALSDPVWDFLDRGGKRWRPSLMLTCCEAVCGSIEPARPLVPLIELIHNGTLVHDD
                     LEDNSDLRRGKPCLHKSFGIDIATNVGSAMYFIPLVILYGRRAHYSDAQCLYIYDLVA
                     QELVRCHCGQAIDIAWHNGRRDDITEDEYLQMCAYKTGVLARLAARLGAYVGGATPQQ
                     VEELGRFGETIGVAFQIQDDILNLVGERFAEGKGRGEDIHEGKRTLLALHALSHASSS
                     QAARLREILAAHPTEQQVLDEAISIIAACGSIDYARRKAVALVEASWEKVATLVQESD
                     AKKRLRAFADFVVQRDL"
     gene            complement(9692..10909)
                     /locus_tag="PLT86_02900"
     CDS             complement(9692..10909)
                     /locus_tag="PLT86_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT35595.1"
                     /translation="MIELEEIQRHLAATSVDGWLLYDFQGINPVAARVAGLGEKMLTR
                     RWFCLIPRQGNPEWLVNQIERQQFADVEGVVRTFSTWQQLVSGLSSLLQGRSRVAMEY
                     SPGGTIPYVSRVDAGTLEIVRNNGCAVVSSADLVQWCEARLSARALATHLYAAKHLET
                     AKESAFEFIFRQMEAGKRISEYDIQQEIIRYFDLHGLVTSSPPIVAAAQNTSDPHYQP
                     TSSRAKLLEEGDLVLVDLWAKTRDPGAVYADITWMAHVSDSVPDELARVFAAVVRARD
                     AAIDFLKRSVARGDIVLGYEVDDVARGSVTKDGYGDRFIHRTGHNIGTEDHGRGVNFD
                     NYETHDDRRVIPNITCSIEPGLYFGFFGMRTEVNVHIGARSVEVSTVPMQRQIIPLQR
                     HFLREATSPSEGL"
     gene            complement(10934..11455)
                     /locus_tag="PLT86_02905"
     CDS             complement(10934..11455)
                     /locus_tag="PLT86_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35596.1"
                     /translation="MNSQQRREYFRQHLKSVLAQENPFLRHITFRYPPTESEWGQFPT
                     EITVNGQHPGILELRVKTTAATPEVQVRFRAKGFSFSPDEDDPSYSVFSAVADKAGAD
                     ELADFVEWAFLVALDSPDDYDPLIRTPVSEQGPSGAPAKPGKGCSSGCRNIALALLWL
                     LLAIFALRFLFGG"
     gene            complement(11495..11893)
                     /locus_tag="PLT86_02910"
     CDS             complement(11495..11893)
                     /locus_tag="PLT86_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT35597.1"
                     /translation="MPGPVSLHHVAIVTTKFDESLKFYRDVLGLGLVHQWKGGDRRLC
                     LLAVGNARIELIEKLTPPDGAGQAAEPPIAHLTFRVDDVDTWTETVRAYGCEITTEPK
                     DVVLGDAPARLSFFVGPNGETLEFFSSKAV"
     gene            12090..12320
                     /locus_tag="PLT86_02915"
     CDS             12090..12320
                     /locus_tag="PLT86_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35598.1"
                     /translation="MKQHQIMLADLPAWTESLGCKTEDLVEMLGWLVDTKSELLNDFS
                     LDMHLVDAIVQCRLRREGRFDRREWVGEDAPE"
     gene            complement(12360..12437)
                     /locus_tag="PLT86_02920"
     tRNA            complement(12360..12437)
                     /locus_tag="PLT86_02920"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12400..12402),aa:Asp,seq:gtc)
     gene            12605..13957
                     /gene="ffh"
                     /locus_tag="PLT86_02925"
     CDS             12605..13957
                     /gene="ffh"
                     /locus_tag="PLT86_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT35599.1"
                     /translation="MLESLSSALDDIFRRLRGRGALSPGNVKDALGEVRKALLAADVN
                     FHVAKEFVKRVEHLAVGQEVLRSISPGQQVVKIVYDELVRLMGSSASTLAIDGAMPAV
                     VMLVGLQGSGKTTACGKLALFLKGKGRHPHLIAADVYRPAAIDQLEQIGKQAGVPVFR
                     SPEGTNPVNIARQGIAAARKAGDDVVILDTAGRLNIDEEMMRELEEISAEVKPQETLL
                     VVDAMTGQIAAEVARDFGRRLDLTGVILSKMDGDARGGAALSVREVSGKPIKFISTGE
                     TLDALDVFHPDRMANRILGMGDIVSLVERAQAAVNQEEARELEKKLRTRAFTLDDFLG
                     QLQQVKKMGPLDQLLGMLPGMNRELLGAKVDDKALGRIEAMIHSMTREERERPEIING
                     GRRRRISTGSGTTVQELNRLLKQFDVMRKMLSKHRPGSKATAAAMSQKLANVSPRRFR
                     "
     gene            14010..14270
                     /gene="rpsP"
                     /locus_tag="PLT86_02930"
     CDS             14010..14270
                     /gene="rpsP"
                     /locus_tag="PLT86_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT35600.1"
                     /translation="MPATMRLKRIGAKKAPSYRVIITDRKNRRDAEAVEEVGFYSPAQ
                     NPPIVTFKKERVEYWLSVGAVPSETVGNLLKNAGISRPKASA"
     gene            14290..14805
                     /gene="rimM"
                     /locus_tag="PLT86_02935"
     CDS             14290..14805
                     /gene="rimM"
                     /locus_tag="PLT86_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT35601.1"
                     /translation="MRPQRVPIGRILRPHGVRGELKVELLSDEATRFDSVGKVTVTRN
                     GVDLGEFEVTGWRRLGRAIGLKLVGIDTPEAALPLRNALLEAPALPAESLPENTYFVY
                     DLVGLHVVSESLEHLGEVVDVVQLPSGELLVVKAGETEHLVPLVQDFVKRVDLEEKVV
                     TLHLVPGLLDL"
     gene            14828..15499
                     /gene="trmD"
                     /locus_tag="PLT86_02940"
     CDS             14828..15499
                     /gene="trmD"
                     /locus_tag="PLT86_02940"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT35602.1"
                     /translation="MEIDVITLFPELFVSPLAVSILGRAQSKGLVAVRPHDLRQWADD
                     ARGTVDDTPFGGGGGMVLMPEPLKRGVEAVKRDHGDGAVILLAPDGEPLTQRIANELS
                     LEQHLILICGHYRGVDERFRARYVDREVSIGDYVLTGGELPALVLVDAVVRLIPGVLG
                     NFESAMEDSFQDGMLDCPWYTRPQVFEGEAVPQVLVSGNHAEIRKWRRQQALERTRAR
                     RPDMV"
     gene            15661..16086
                     /gene="rplS"
                     /locus_tag="PLT86_02945"
     CDS             15661..16086
                     /gene="rplS"
                     /locus_tag="PLT86_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT35603.1"
                     /translation="MDLVDLVDRKHRREEERNENGEVTFAIPEFKTGDLVDVHVRIVE
                     GDNERIQVFRGTVIQRHGGSSSNASFTVRKVTAGIGIERIFPLHSPRLAKIEVVRRGR
                     VRRSRIFYLRDLRGKAARVTEKRDLRTAEQKAAAVETAQ"
     gene            complement(16175..17818)
                     /locus_tag="PLT86_02950"
     CDS             complement(16175..17818)
                     /locus_tag="PLT86_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35604.1"
                     /translation="MAEEHEIRVRVTPRDSEQVNRVFRILTARINERYTTAITQSSEN
                     PRLVLNVEPSLAAEAFRIREDGRATVIEGGSPLGLLYGVGKFLRTSRFEGAFTASPWR
                     GVSAPRGSLRGMYFATHFHNWYHVAPENEITRYVEDMALWGVNAIKTAFPFINLRDWE
                     DPETEHALTMTRKFARTARDLGLKFAIGINNTMFSGAPSHTRAVPLPDPTHRRGNSGH
                     PVCPSNPEGRDYLLANARRLFEELSGVGLDLVTFWPYDEGGCACDRCSPWGCNGFLRL
                     SHDFAELAREYFPNIKTVLSTWMFDTPPEGEWEGLTNALKDGNNWVDYILADAHEDFP
                     RYPLDVGVPGNLPLVNFPEISMWGNWPWGGVGANPLPARFQRLWDQVKHIVRGGFPYS
                     EGIYEDMNKAIVAQFYWTPERSARDTLREYIAYEFGGKAIEETVALVEALEMAATRSY
                     TKQLVDVGLVRTARELADKVHEKMPAWARTGWRWEILRLRAILDHERFAGEGFATSEA
                     ERALERLIEIYHCEMETDDPYHHRVRPPLARAVSRRGNL"
     assembly_gap    18125..18224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18225..18889
                     /locus_tag="PLT86_02955"
     CDS             <18225..18889
                     /locus_tag="PLT86_02955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT35605.1"
                     /translation="LADPRHESASRWKAAMLAATYLETGELCGSHLTIAEIGGGFGTV
                     VNALGLILGSERLTVYDISAKACEEGKKAYPGTTFINAPYGEGNRSSWDIVVLCDVTE
                     HVEDEDALLRTVAEDARFVLLKIPIEKCLWRVWRRLKGKPPGFSWGPDHQSGHLRGYT
                     LWSAKRLVRRHFHVVNTSLSESVWFYGSARSLRLAIRVGKYLSVMIFGGSLCVLARPR
                     AR"
     gene            complement(18903..20000)
                     /locus_tag="PLT86_02960"
     CDS             complement(18903..20000)
                     /locus_tag="PLT86_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35606.1"
                     /translation="MTDTMTPRERWKAAVRMEPVDRLPFWAKLPGNYPGFHGAPFSRM
                     TVDALHEWIGSDPTVHLRTGIRTVHRRASVNTVIEGDFRKTEYRIGGQCLTLVSQIDH
                     ASFSWHPIRFPVKTRDEINILADVFEDSAAEVDPNELAEAREQSRRVGERTITAQDIG
                     ESPLMVWVEWLAGIQNAHYLLNDFPAYVERLFEAIHRDLVRKTEILSEHGPADVFYLT
                     ENTSTTLISPEQYRRYCARHISEYVRIVRNAGRHLILHMCGHLKALLPDLARIPVSGF
                     EAFTTPTVGNTTLAEGRRACPDKCLIGGTNAALWLEPAEAIIARIEAELDALPHHRGI
                     VVSSAGMLPQACRPETVKRVAEWVKGYRVVC"
     gene            complement(20138..21343)
                     /locus_tag="PLT86_02965"
     CDS             complement(20138..21343)
                     /locus_tag="PLT86_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT35607.1"
                     /translation="MLILEIIRVAFGALLANKGRAFLTMLGIIIGVGAVIAMMAIGAG
                     AQKAVQDRIASMGVNQLRVFADRTTIRGVTRYRSLTTDDAQALRNEMTLATAVVPDMG
                     GQYQVKYGNKNNSVRITGTTPEYFSLRSYQLAAGRFFTNGDMSASQRVAVLGSVAAEN
                     LGIGPQDVGSTVRIGGMIFDVIGIMESKGSQGGWDNPDENIFIPITTAQYRLMGNDRI
                     NGVTVQVVAQEWMLAAQGEMERILRRERQLRPEQQNDFRFFNQQELATTFAESTKIFG
                     LLLASIAAVSLIVGGIGIMNIMLVSVTERTREIGIRKALGARRSIILLQFLLESITLC
                     VLGGAIGMGLGYWVAKMMASSGGWEVIVTPQSLLLAVGFSVGVGLFFGIYPASRAAKL
                     DPIEALRYE"
     gene            complement(21356..22786)
                     /locus_tag="PLT86_02970"
     CDS             complement(21356..22786)
                     /locus_tag="PLT86_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT35608.1"
                     /translation="MTGIVNRRAAVLFLLAVSMAGCSMPGCNKSKEGESVDLFTTTKV
                     ERRDIEIKVTATGSIEPIRVIEIKSKASGEILALPVETGDVVSAGALLAQVDTTDVAA
                     AYRQARADLEVARVRESVSRKSLDRAADLLARGMTSTEEYDQARLDYATARANVIRAE
                     TDLETKTERMIETIVRAPSTGTILKKNVEKGQIIASVGQVNAGTTLMTMADLSSVQVR
                     AMVDETDVGKLRPGLPVTVTVDAFPGRNFTGEIQKIEPQSVEEQNVTFFPMIVQIPNS
                     EGLLRPGMNASVDVQIMKKTGVPSLPNDAIKTLQNAGVVGMFLGLNPDSVNAFVDANK
                     AKFMDHDSTRRGPGRRGGPGPGMANAPSESSARGARPSGATARARPSRGGSMHFSSRD
                     IPQGPAPGVMAAGSVGVVFVPDSATTKVVLVKTGIQNWEYTEITSGLKEGDTVYLPPS
                     AALAMQSQAMRDRLKQFSVIPGRSSR"
     gene            complement(22783..23478)
                     /locus_tag="PLT86_02975"
     CDS             complement(22783..23478)
                     /locus_tag="PLT86_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35609.1"
                     /translation="MLIEMRGIEKVYKLGSERVNALRGIDLDVSEGEYVALTGPSGSG
                     KSTLMHLIGCLDSPTAGRYLLAGSDVSSMSDAALARVRNRQIGFVFQTFNLLPRATAL
                     HNVEIPLIYGGFGTRERRRRATAALERVGLADRMKHQPNQMSGGQRQRVAVARALVTD
                     PSILLADEPTGNLDTATGNSILELFAQLHASGQTIVLVTHEADVAAHARRHIVLRDGK
                     IESDDANKKESAG"
     gene            23758..24558
                     /locus_tag="PLT86_02980"
     CDS             23758..24558
                     /locus_tag="PLT86_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT35610.1"
                     /translation="MPLSQWGRILVDEMEAAAVPPGDLWIWPLGGASLAVKSARSMVY
                     IDPYTGSPSSGVWIRMVAVPFDPRDVRIADAVLSTHDHDDHCHGETLRPFADNTGALF
                     IGPASSASKMRSFKLPETRIMSARHGDSFRFSDITVEAVEIVDHSDPTSVGWLVSVDD
                     GPVLFDGGDGMYDDYFKKVAETRRVSAATLSIAGLTGDGRKIYMDARELVAAAEDLGV
                     DLLIPKHWDLWRNVWLDPWEVVIAAQARRARFRVHIPRLGEVIRLPAR"
     gene            24750..>25096
                     /locus_tag="PLT86_02985"
     CDS             24750..>25096
                     /locus_tag="PLT86_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35611.1"
                     /translation="MMRRFLAASLLLAVALPAVAAVSVGQKGSDFSLSNVDDVRFSLA
                     TPRTTPLVLIFTTKDLGNVSQPWRDSLTTTLGSSAQIQTVLDLGDVSRFLHSVARTRI
                     RAQKSKAILDWDG"
BASE COUNT         5128 a         7305 c         7312 g         5251 t
ORIGIN      
        1 cttcactgaa gaaaatgtgg ccggacattt ccccggcaag aggtgtcccg gtctctttca
       61 tcttcgcttt gataagcgaa tggcccgttt tccacatcac cggtttcccc ccgtgccggg
      121 cgatatcttc aggcaatgcc tgcgagcatt tcacgtcgaa aatgatctcg gctcccggta
      181 ccctcgtaag aacgtcgcgc gcaaaaagag cgaggagcct gtccccctcc actatccggc
      241 ctttgtcgtc aatcacaccg atccggtctg catcgccgtc gtatcctata ccgatatctg
      301 caccggtttc tttcactttg gagatgaggt cgtgcatgta cctggccatt gtcgggtccg
      361 gcagatggtt gggaaacgtg ccgtcaacct cacaaaaaag cggggtaact tcacatccga
      421 ggctgcgaaa aatcgcgggg gcaatctcac ttgcgcatcc atttccggcg tcgatgacca
      481 cgcgaagacg ccgtgatatc gccgtgcgct gaaggatatc agatgtgtag gcggagcgga
      541 tatcctcttc gctgtactgc ccggtgcccc tctcgaaatc cgatgtttca atcaaacggc
      601 ggagaccctg aatctgctcc ccatagatcg ccgaaagacc gtggcacatt ttgaacccgt
      661 tgaactccgg cgggttgtga cttcccgtga tctggacgcc gccgcctgtc ttcagatgga
      721 gaatgccgaa atagagggca ggggtcggca cgacaccgag atcgcgcaca catattcccg
      781 tgctcagcaa tccttccacg agcgcttcgc gcaacctcac gccggagggg cgcacgtcgc
      841 ccgacacaga gcgacgattc gccacgcctg atcagctccg tcccaagcgc tcggccgatc
      901 tgcattgtaa ggtcactggt cagatcggtt tcgtgaagcc cgcggatatc gtactcacgg
      961 aatatgttcg ggttcattcg cacaaccctt ctggattctg tcacaggata agcaccgcgt
     1021 ggtaacgccc ctctcctacg cggtgcaccc tggtgaccca ttgcccgtcg ggcgccacca
     1081 cgccgctgga gcagttgatg ccgcgatccg ggcgagcggc gtttgccgtg cacacataca
     1141 tgtggctttc ctctgcgcgc agggagatat tggactcatg gtaaggcgta tgccgggcgc
     1201 cggcacccga attgatgctg tggaacacga ttctcgtccc cttctggccc aactggtaca
     1261 tgagccggag gtccttgtga ggaccacaac ccggtgtagc ccacatgtcg ttacagatga
     1321 gcattccgca ggtaatcccg ttccattgaa acgtgcggag ttctttaccc ggaacgcaga
     1381 aggcgcggtc ccctgattca ccgtcaatac ccgtggggat catcttctcg tgagttccca
     1441 gcagatctcc gcactcatcg tacgcccgct gctggatgaa ggcctctcct ccttctctcc
     1501 accccgtcga aatgattgcc cccacacgca gtttgcgcaa cgcggtgcgg atcgtctcca
     1561 ctgcgccctt caccttctcg aagtcgtagt tcccgtgata gccgctgagc gacatctcgg
     1621 gaaacaacac gaactgcgcg ccctcctcgg cggcgatcga caggttgtgg aggattttcg
     1681 gaagattggt gtcaagatcg gcggataccg ccatctgaat tcccgcgatt ctgagcgttt
     1741 tcgccattca tcaacccttc aaggcagact gacttttcct tcgcgcgaac tgaaaaacaa
     1801 gaatagaggg gtgcccccga ttttgccaaa catcatccgt catgcgttcg atcggcgtgc
     1861 gaccggtcag tgcttctgcc ccggaagcag tccaaaatag agcgtgcccg tatcgaccgg
     1921 gatgcccagc aatttcaggt acagaaaaag ctgcattttg tggttcaaga aatgctctat
     1981 tatcaacccg tacgccatcg tttcgagagt ccctttcact ccccacggca tctcgacgat
     2041 tcggtgctcc cagtcttcga tactgacttc ggataaagac cttttgacct cctcgacggc
     2101 tttggtcaac cgttccttgg ccatgcccgg cgtgatagaa gcggggtcat cgttgtcgcc
     2161 ccagcaccgg gaagggtcaa agtcaccggt caccaatccg tgcacgccgg ttcgcgccga
     2221 aagatgatga atcaactgcc ggattgtcat catgttggtc gccggtgccc aatctaaccg
     2281 ttcgactgga atcagactca aaagtgcggg ggccggtttg taggtcctgt ccacatgctc
     2341 gagaaaacgc gtcgggttca tcggtgtctc ctttgtctgg aggtgtttga ggaggggtaa
     2401 actgaagcac gcattcgcga aggagtgtgt caaggcgacc cgcccagtag ttacctttgt
     2461 taacggaacg caccacggaa tgaccaagat tcgaaacagg agccggttgt ttgggcgaat
     2521 tcactttctg gctgcatcaa cttgcggcgc atcatcccgc agaagcgtat gccatcctgt
     2581 ttctcagtgc agtggttgag aatcttttcc ctccggtacc gggtgatacg gtgacggttt
     2641 tcggcgcata cctggtaggt cgcggctcgc tagaactgtg gccggtcatc tgggcaacgg
     2701 tggcggggag cgcttcgggg tttctcggcc tttacgtact cgggcgcacg tacgggcgcg
     2761 gcgtactttc acgcctccgg tgggtacaac gttcgatgcc acgaatcgcg ctggcagaac
     2821 gactatgtcg ccggcgcggt attcttgtgg tcctggcgaa tcgatttctt cccggccttc
     2881 gttcggttgt ctcgatcacc gtcgggttgc ttcgaatgcc cgcgtggcag gtggtcccgg
     2941 ctgcagtgtt cagcatattc gcctggaacg gccttctcat ctgggggggg cttcttgcgg
     3001 gcgaaaactg ggaacgagcg gtggagatcg tcagccgtta caacacctat ttcggtgcat
     3061 tgctcgtggt tatggcgctt ggtgtatccg cgtggtggtg gggaacgcgc cgccatcggc
     3121 gtccaattct gcatggagag aatccgcgat gagtcttcag gtttacaaca gcctcaccag
     3181 gaccaaggaa atcttcgagc cgatagtacc cggcagggtc ggcatttatg tgtgtggacc
     3241 gactgtctac ggtgactccc atctggggca cgcgaaggct tacatctcat ttgatgtgat
     3301 tgtgaggtac ctgcgttttc tggggtacaa ggtccggtac gtccagaata ttaccgatgt
     3361 gggacacatg acggacaatg acgaggacca gggcgaggac aagatcgcca aaaaggcagc
     3421 cctggaacgg atcgaaccga tggaagtcgt tacccattac atgatgagct atttcgatga
     3481 catggccgcg ctcaacgttg cgcgtccgga cataatctgc cttgcttcag gtcacattcc
     3541 agagcagatc gcacttatcc aacgacttct cgacgccggg gtggcgtacg tctcaaacgg
     3601 atccgtctat ttttcggtcg atcagttccg cagctatggg aaactttcgg ggcggcgcgt
     3661 ggaagagctt gaggaagggg cccgggtggc tgtcaacctc gagaaaaaga acccggccga
     3721 ttttgcgctc tggaaaaagg cggaaccggg ccatctgatg gtatgggata gcccctgggg
     3781 caagggtttt ccggggtggc atctcgagtg ttccgccatg tcgatgaagt acctcggtga
     3841 gacattcgac atccacggag gtggattgga aaaccaattt ccgcaccatg agtgcgaaat
     3901 agcccagtcg gaagcagcga cgggcaaacc ttttgtgcgc tactggttgc ataataacat
     3961 gctcacgatc aacgggcaaa aaatgggcaa aagccttggc aacttcgtca cgatcaagga
     4021 aatgcttcgg aagatcagcc cggaggcgct gaggctgttc atcctgatga gccattaccg
     4081 ttcagtaacg gacttcagcg acgaagcgct gaacagcgcg gagaagggta tggcccggct
     4141 ctccgctacg gtgcaggcgt tatcagagcg cctgccgggg gctccagaag gtgcagcgga
     4201 tcccgaaact gatgctgagc tggcagagat acgccgcaaa ttcgtcgttg cgatggacga
     4261 tgacttcaac actgcggaag cgatcgcaca gcttttttcc gtggtcaggc tgaccaacag
     4321 tgttctttcg gcgccgaatc cggcaaccag aggaacattg gaactactga acacgcagtt
     4381 tgacgaactc ggggaaggcg ttctgggcct gaggctgaag gcacgggctg agggaagtga
     4441 ggacctgacg gatcagataa tgcaggttct gatccagatt cgtgaaaaac tgaggaaaga
     4501 aaagcagtat gcgatggcgg acgagattcg ccagatgctc gcagaagtgg gcattgtatt
     4561 gaaggacgaa aaagggcgaa ccacgtgggc tcgctcctga agcgcgcggt tactggacgt
     4621 agaagaaatt cgcggggtca accgcccgac cgtgatagaa cacgctgtaa tggagatgcg
     4681 gacctgttga ccggcccgta ttcccaagtt ccgcgatcgt ctgtcctctt gctacccgct
     4741 gcccctctcg cacgagcgct ttcgccaggt gaccgtacac ggtgctgtac ccgtacccgt
     4801 ggtcgatctg cacaaccaat ccggtgtatc ctccccacga cacatcggca acaacgccgt
     4861 ccgcggtaga aacgacgctc gtgcctgtcc ttccggccag atcgatgcct ttgtggattc
     4921 gtcgcctccc tgtatagggg tcggcgcgat acccaaaaac ggaggaaaca tatacctgcg
     4981 ccaggtcaac tggaggaata ctcgggaggt ggtcacggaa ctcgatgttg ttggcgagcg
     5041 cacgctctat ttcccggaaa ctttcgcgct ggaacttcgc ctgattcaga agcgactgga
     5101 tgtccgagtg gacctccata ccgaggcgcc ctgcttctgt gacaacatca tccgaggaga
     5161 aaagatcctc atcgacgtcg aggctgccga ttcctgcggc ccgtacgtcg ctgtcaacct
     5221 ccgggaggtt tgccatcacc cggagcatct cctctttgcg cacgaggttg cttacctcag
     5281 ctttcagctc cactaccccg gaggacatgt cttgcaagcg ggaagcaagt tgcgcgtttt
     5341 cctgcttcaa acgctcgaca gcctttgcct gactggttcg tatgtggaac ccaattgcgt
     5401 aggtgagggt caacagcact atcgcaacga ggatcaaacc gccaaccaga agagcgcgat
     5461 cggtaacagt gatctcgcga acacgctgat ggctgtgcgg cacaatcatg atcgtgagtc
     5521 gccgcaaccc catacggttt cctctcaatt atggcggcac gagcgcggaa cgaggctgcc
     5581 tgagcgcaac ccaccgggac gaaacaagga catcgagtcg cccggccttc ctcctgaacc
     5641 ggcttgccgg tcctcacacg agcaagacgg aattaaccta gtcatgaata cgttcgctgt
     5701 caagccatgt gacggaacag gccctcaaag tgcgactgca cggtggaggt caagggatga
     5761 cggcaacgtc cgtaaattgg ccgcaaaacc ggcgggcaac aacccgcggg attggtgtcc
     5821 ctgcacccgg gtcgtctgcg cgaataagtg gtggaggttt tccagagtgt tcggaggggt
     5881 gagatcactg aggatttcgg gcgaaaccgg gctaatgacg cgggaaaggc gcctccaaac
     5941 gagacataag ccagcaagaa gccatttggg aattcaaaaa tccggtgcga ttgcagaggg
     6001 gactcgacgt ggcaaggggg gacgtgcggc gccacgctgc acaccccccc catcgccact
     6061 gagtctgtct cagccctggg tgcggttact cagaacacgc atgagcaact cgtccagcga
     6121 ccaccttcct cctcccttga tcattaccgc gaggccgacg ccgaacagaa tgagcgtaaa
     6181 ctcgatgcca ttctgctgtg cgaagaaccc gttgggcagg tggacggctg tcatggctac
     6241 taacatcacc atgccaatgc cgaacgcggc tacccgggtg agaaaaccga agatcactcc
     6301 cagtgcgccg aaaaattccg cgaagatcgc gagaaacgca aacaccggag ggattcccat
     6361 tgaaccgaaa aatcccatcg tcgccgagaa cccgtgcccg ccgaaccatc cgagcatttt
     6421 ctgggcaccg tgggggaaca tgacaattcc aagaaacacc ctcgccacaa accccgcgag
     6481 gtcatttctg tcagtctcca gcagccgttt caacatggtt tcctcccttc gccgtattgc
     6541 acacgcttcc aacttggaaa aacgtatctt agatggcttc caatactacc cggaaagacg
     6601 gcgtacgagt tccacggcgc gtcagcctgg gtatttcccc cgtggcacga gcaatctcaa
     6661 cgcgtgaggc acaacggaga aatgaaggac tgctggtccg tcgaatggct cgccgtccgc
     6721 gtggtacggg cccgggtcct ggcgatggat cgtgaactcc gtggccgtca ttcgccgaac
     6781 gagaggtgac cgatccagag agcccgtaaa gagcctcggg aacgcgatca ggagacgaag
     6841 gaacgaactc gtctcaacga tggtgatgtt caaaaggcca tcaaacggag atgcaccggg
     6901 tgcgattttc gctccgccac cgtactgctc cgtgttcgca acggccagga cgagcggttt
     6961 tgctgacagg gacatgttgc ctgattgaat ggtgactttc tgaggacagt acgcgaccca
     7021 ggcagcgaat ccgtggcgga aatatggcca gattccccga cgatggttcc ccgcagcaaa
     7081 tcgccgactg atttctgcgt cgaggccgat gccggctgtc gcgaaaaagt gacgaccttc
     7141 tacggtccca acatcaacgc tgatctgttc gctgtcaacc agtgagcgaa tcgcgcgaag
     7201 cgggtccaac gggattttga aggatctggc gaacccgttg ccggaaccac acgggatgac
     7261 tgcgagggct acctgcgttc cgaccagcgc cgcggatact tcgtgtaccg ttccatcgcc
     7321 accggcggca acgatgatgt cgctggcagg aacgagagat cgtgcgatct gcgtagcctc
     7381 gcccggcccc gtagttgtga cggtccgaag ttcccacccg tgcatgctgg caacactgcg
     7441 cagcccggca atcacgtgtt ccgcatgagg gcgccttccc gcccgcggat ttatgatgca
     7501 ggcgacacgc attggcctat tccggaagcg ggattcgaac agcagtatgt gtgtccatcg
     7561 agcggtaggc ggccatgcag atctcgagcg agcgaaatgc ctcgcgtgcg tccgtaagag
     7621 gtctcgtgcc ctgcgcgatg cattcccccc agtgcctcaa ctcctcgcga aatgaatctg
     7681 tccacgtgtc gccttcggga gcgtggaact cctcctgctc cccggcagtg cggaggctga
     7741 cgacgtcacc ccgcagcgtg gagacagttc ccctcgttcc atacacgacc agaaactggt
     7801 accacggatg gttcggcgcc gcccaggaga tatcgagcgt cccgatgcag tccgggcatc
     7861 cttccttgcg cggcgcgaat tccacgatga cagaagccgc ggtctccgtc tcaaggactt
     7921 ccgggagttt cacgccggtc gcaaatacgc tcgttgctgt tccacaaaac cactgcagca
     7981 gatcgagcat gtggatccct gaaccgatca acgctccccc gccggcgagt tgtccgttgc
     8041 ggtaccaggc attcgccgcg aaacgcgggt tctggttgtg gtccgttcgc accatgtaca
     8101 cgtcgccaat ccgcccctcg tcaacgattt tcttgatcgt cctgacggac cagcggtgcc
     8161 gttcattgaa tgcgaccatg aacactgcgt tggaggcctc cgcctcaaga acaagctcga
     8221 ttccctctga aagggtgatc gccagcggct tttccagaca cacgtgcttt cccgcgcgga
     8281 gtgcggatac cccaacgagc gcatggaggt ggtgcggcag aaggatgtcg actgcatcga
     8341 tttcggggtc ggcgatcacc tctcgccaat cggagcagat gcgcggttca ccgaatggct
     8401 ccgcgagctt ccgggccgcc ttttcgtttg agtcgcagac tgcacgaacc tcaaacagcg
     8461 cgggcagatt cccaaaaccg agcgcgtgcg cgtgagaaat gcccccacaa ccgatgattc
     8521 cgacccgaaa aggtggtgtg gttctccgca cggtagcttc ctttccggaa agggcgtgcc
     8581 cgtttctgcc tcttccaaag atcttcggga aacccgctcc ctcagggctg cggtccctaa
     8641 aggtctcttt gaacaacaaa atccgcgaat gcgcggaggc gtttttttgc gtcggattcc
     8701 tgaacgaggg tggcgacctt ttcccagctc gcttccacga gagcgaccgc tttgcggcgt
     8761 gcgtaatcga tcgagccgca cgcggcgatg atcgagatgg cttcgtcgag aacctgctgt
     8821 tccgtgggat gcgcagccag aatctcccgg agacgtgccg cctgcgagga actggcgtga
     8881 gaaagtgcgt gtaacgccag cagggtgcgc ttcccctcgt ggatgtcctc cccgcgtcct
     8941 ttgccttctg cgaaacgttc accaaccagg ttcagaatgt catcctgaat ctggaacgcg
     9001 accccgatgg tttctccgaa ccgaccgagt tcctccacct gttgcggcgt tgcaccccct
     9061 acgtatgcgc caagacgcgc cgcaagcctc gccagcacgc ccgttttgta cgcgcacatc
     9121 tggagatact catcttctgt gatatcgtct cttcgtccgt tgtgccacgc gatatcaatc
     9181 gcctggccgc aatggcatcg gacaagctcc tgagccacga ggtcgtagat gtacaggcac
     9241 tgggcgtccg agtagtgtgc gcgtcggccg tacaggatta cgaggggtat gaaatacatg
     9301 gcgctgccga cgtttgttgc gatatcgatg ccgaacgact tgtggagaca gggcttcccg
     9361 cgccgcagat cgctgttgtc ctccaggtca tcatgaacca gggtaccatt gtggatgagt
     9421 tcgatcagcg gtaccagagg acgtgcgggt tcaatgctcc cacatactgc ttcgcaacat
     9481 gtcagcatga gcgaaggccg ccacctcttg ccgccgcggt cgaggaaatc ccacacggga
     9541 tccgatagcg cggctgtcag agtctcctcg tccacggaat acgtgcttct tcccagcacc
     9601 gcttccaccc attccgccgt gatcgagcgc ggtagaaccg cctccagttg ctggttgatc
     9661 cgcgcggtct gttcttggag gtaatcgagc atcacaagcc ctcggaaggc gaagtggctt
     9721 ctcgcaggaa atgcctctgc aggggaatta tctgccgttg catcggtacc gtactgactt
     9781 ccacgctgcg cgcgccgata tgcacgttta cctctgtccg cattccgaag aaaccgaaat
     9841 acagaccggg ttcgatggag caggttatgt ttggtattac gcgcctgtcg tcatgggttt
     9901 cgtaattgtc gaaattgaca ccccgcccgt gatcttccgt gccgatgttg tgcccggtcc
     9961 ggtggatgaa acgatctccg tacccgtcct tcgtaacgga accacgggcg acgtcatcaa
    10021 cttcgtaccc cagaacgatg tcacctctcg ccactgagcg cttgaggaaa tctatcgcag
    10081 cgtcacgtgc gcgcacgaca gcagcgaaca cccgcgccag ctcgtccggt accgaatcac
    10141 ttacgtgggc catccacgta atatccgcgt agaccgcgcc cgggtcgcgg gtttttgccc
    10201 agaggtcgac caggaccagg tcgccctcct ccagcagctt cgctctcgat gacgttggct
    10261 ggtaatgcgg atcgcttgtg ttctgcgctg ccgccacgat cggcggggaa ctggtgacga
    10321 ggccgtggag atcgaagtag cggatgattt cctgttgaat atcgtattcg gaaattcgct
    10381 tacccgcctc catctgtcgg aaaatgaatt cgaacgccga ttctttcgca gtttcgagat
    10441 gcttcgccgc atagaggtgg gtcgcgagcg cacgcgccga aagccgcgct tcacaccact
    10501 ggacgagatc tgccgacgaa acaacagcgc aaccgttatt gcgcacgatc tccaaggtgc
    10561 cggcatcaac ccggctcacg tacgggatgg tgccgcccgg actgtattcc atcgcgacgc
    10621 ggctgcgccc ctggaggagc gaagaaaggc ccgatacgag ttgttgccag gtggagaacg
    10681 tgcgcaccac tccctcaacg tcggcaaatt gctgacgctc aatctggttg accagccatt
    10741 cggggtttcc ctgccgtggg ataagacaga accaccggcg cgtaagcatt ttctcgccca
    10801 gtccggccac ccgggcggcg acggggttga tcccctggaa atcgtacaac agccatccat
    10861 cgacggaggt tgcggcgaga tggcgctgga tttcttcgag ttcgatcatt tctggaacaa
    10921 gccacgcaat gggtcagcca ccgaaaagaa aacgcagtgc aaaaatagca agcagcaacc
    10981 agagaagagc gagagcgatg tttctgcatc cgctgctgca ccccttaccc ggtttcgccg
    11041 gcgcgccgga aggtccttgt tctgataccg gagttctgat cagtggatca tagtcatccg
    11101 gggaatccag agcgacaaga aatgcccact ccacaaaatc tgcaagctca tcagcaccgg
    11161 ccttgtccgc aaccgcgctg aaaactgaat agctggggtc gtcttcgtca ggggagaaac
    11221 tgaacccttt tgcgcggaac cggacttgaa cctccggggt tgcagcagtc gtcttcaccc
    11281 gcaactcaag aatgcccggg tgctgcccgt tcactgtgat ttcagtaggg aactgccccc
    11341 attcggattc tgtgggaggg tatcggaagg taatatgtct gaggaacgga ttctcctgtg
    11401 cgagaacgct tttcaggtgc tgccggaagt attcgcgcct ctgttgcgag ttcatgggtc
    11461 cccccacatg ccatcctttg cggcacgttt ccgttcagac agcctttgac gagaagaact
    11521 caagcgtttc gccgttcggt ccgacaaaaa acgaaagtcg cgcaggagcg tcgccgagaa
    11581 cgacgtcttt tggctcggta gtaatctcgc atccgtacgc ccgcacggtc tccgtccacg
    11641 tatccacgtc atccactctg aacgtaaggt gcgcaatcgg tggttctgca gcttgacccg
    11701 cgccatcggg aggtgtgagc ttctcaatca gctcgatgcg cgcgtttccg actgctagaa
    11761 ggcacaggcg acggtcgcca cccttccact ggtgcacgag gcccaaaccc agcacatcgc
    11821 ggtaaaactt gaggctttcg tcaaactttg tcgtcacgat tgcaacgtgg tgcagggaaa
    11881 caggacctgg catgcggttc tcctctggcg atttgcggaa ctggtgcagg cggcatttgg
    11941 actggcgcct tttttttcaa atatccggcc ccggaggttc tgagtctagg ggggaacacg
    12001 cgtcgcggcc ggtggcttct gaatggggcg tgccaccaga cgacattcag ttacagaaaa
    12061 tcgccgcgca tttgcgacgt ttgcgccgta tgaagcaaca ccagatcatg ctggccgatc
    12121 ttcccgcgtg gacggaatcc ctcgggtgca agaccgaaga cctggtggag atgctgggat
    12181 ggctggtgga cacaaaatcc gagttgctga acgatttcag ccttgacatg caccttgtgg
    12241 atgcgattgt tcagtgtcgg ttgcgacgcg aggggcgatt cgaccggcgc gaatgggtgg
    12301 gagaggacgc accggaatga aaaaccctcg cagttgcgct gcaagggtca agaaacacat
    12361 ggcggggtag acggggctcg aacccgcgac ctccggattg acagcccggc actctaacca
    12421 agctgagcta ctaccccgcg aacaggaata caaagaatac ggaactgccc ggatgctgtc
    12481 aatagtgctt cgatgtcgtt cgttgcagcg aacaaccgga agcccgttga tcatttgcaa
    12541 tgatgaattg cgctgactat ctttataagt ctgttttgtc gcgacacact taccggcatt
    12601 tgccatgctt gaaagcctct cctccgccct tgacgacatc ttccgcagac tgcgcggaag
    12661 aggtgccctt tcgccgggca atgtgaaaga cgcactgggc gaggttcgca aggccttgct
    12721 cgcagcggat gtcaatttcc acgttgcgaa agagttcgtc aaacgggtgg aacacctcgc
    12781 ggtggggcag gaagtccttc gcagcatcag cccgggtcaa caggtcgtaa agattgtgta
    12841 cgacgagctg gtgaggttga tgggatcctc cgcgtcgacc cttgcgattg acggagccat
    12901 gcctgccgtg gtaatgctgg tagggctgca gggttcgggg aagacaactg cctgcgggaa
    12961 gctggcttta ttcctgaaag ggaagggccg ccatccccac cttattgccg cggacgtgta
    13021 ccgccctgcg gcaatcgatc agctcgagca gatcggcaag caggcaggcg ttccggtttt
    13081 tcgttcgcca gagggcacaa accccgtgaa tatcgcgcgt cagggtatag ccgccgcgcg
    13141 aaaagccggt gacgatgtcg tgattcttga cactgcgggg cgtttgaata tagacgaaga
    13201 gatgatgcgg gagctggaag agatttccgc cgaggtcaag ccccaggaga cgcttctcgt
    13261 cgtggatgcg atgaccggac aaatcgctgc cgaagtggca cgtgatttcg gccggcgtct
    13321 ggatctgacc ggcgttatcc tttcgaagat ggacggcgac gcacgcggtg gcgcagcgct
    13381 cagcgtgaga gaggtttccg gaaagccgat caagttcatt tccaccggtg aaacccttga
    13441 tgcgctggac gtatttcatc ctgacaggat ggcgaatcgc atacttggaa tgggcgacat
    13501 cgtctcgctt gtcgaacgcg ctcaggcagc agtgaaccag gaagaagcac gggaactgga
    13561 aaagaaactg cggacccgcg ccttcacgtt ggatgatttc ctgggtcagt tgcagcaggt
    13621 aaaaaagatg gggcctttgg accaacttct cggcatgttg cccggcatga atcgtgaact
    13681 gctcggcgcg aaagttgacg acaaggctct tggtcgcatt gaagccatga tccattcgat
    13741 gacgagggaa gaacgggaac gcccggagat catcaatgga ggccgtcgcc gacgcatttc
    13801 aacggggtcg ggaaccacag ttcaggaact gaatcgcctt ctcaagcagt ttgacgtcat
    13861 gcggaaaatg ctgtcgaagc atcgaccggg aagcaaggcc acggcagcgg cgatgtctca
    13921 gaaactcgca aatgtctctc cgagacgatt ccggtaggat attccggcgt cgttccggat
    13981 ttcgtgcact tctttcagag gagttacgcg tgccagcaac aatgcgtctg aaaagaatcg
    14041 gcgcaaagaa agctcccagc tatcgggtga tcattaccga ccggaagaac cggcgtgatg
    14101 cggaagcggt ggaagaggtc ggtttctaca gccccgcgca gaatccgccc atcgtcacgt
    14161 tcaagaaaga gcgtgtggag tactggctga gcgtcggtgc ggttcccagc gaaaccgtgg
    14221 ggaatctcct caaaaacgct ggcatttcac ggcccaaagc atctgcctga cggcgccgga
    14281 ggtccgcgaa tgcgcccaca gcgggttccg atagggcgta ttcttcgccc gcacggcgtg
    14341 cggggcgaac tcaaggtgga gttgctttcc gatgaggcga cccggttcga ttccgtcggc
    14401 aaagtgacgg tgacgaggaa tggagtcgat ctcggcgagt tcgaggttac cgggtggcgt
    14461 cggctgggca gggcgatcgg cctgaagctg gtgggcatcg acacaccgga agcggcattg
    14521 ccgttgcgca acgcgttgct ggaagcgccc gcgcttcccg ccgaatcgct gcccgaaaac
    14581 acgtacttcg tgtacgatct tgtgggtttg cacgtcgtgt ccgagagtct tgagcatctc
    14641 ggagaggttg tcgacgtggt tcagttaccg tcaggcgaac tcctcgtggt aaaggccggc
    14701 gaaactgagc atctggtccc gctcgtgcag gatttcgtaa aacgtgtcga tcttgaagaa
    14761 aaggtagtga cgctccacct cgtgccgggc ctgctggacc tgtgagagcg cggcggaggc
    14821 gacggacgtg gaaatcgacg taataacgct ttttcctgag cttttcgttt cgccgcttgc
    14881 cgtcagcatt ctcgggcgcg cgcagagcaa gggtctggtc gccgttcgcc cgcacgatct
    14941 tcgccagtgg gcggatgatg cacgtggaac cgtagatgac actccattcg gcgggggcgg
    15001 cggcatggtt ctgatgccgg aaccgctgaa aaggggcgta gaggccgtaa aaagagacca
    15061 tggcgatggt gcggtcattc ttctcgctcc agacggagaa ccgctgacgc aacgtattgc
    15121 gaacgagttg tcgcttgagc aacatctcat cctgatttgc ggccattatc gcggcgtgga
    15181 cgaacggttt cgagcgaggt acgtggacag ggaagtatct atcggcgatt acgtccttac
    15241 cggaggggag cttccggctc tcgtgctggt ggacgccgtc gtgcggctga ttccgggtgt
    15301 gctgggcaat ttcgagtccg ccatggagga ttcgtttcag gatggcatgc tggattgtcc
    15361 ctggtataca cgtcctcagg tgttcgaagg cgaggcagta ccgcaggttc ttgtgagtgg
    15421 aaaccacgcg gaaatccgga agtggagaag acagcaggcg cttgaaagga caagggctcg
    15481 caggcccgac atggtgtaga tcgaaagcct gcacccctcg aacgcgagca tcgggctccc
    15541 ctcagtcggg tgcaggtctc ccgaacggca aaacgtattt gaatgcgcgg tgaactgcgc
    15601 gctacagggc actcagtgcc ggtgggacaa aacaggcact acgtgccgaa tggaggaaac
    15661 atggatctgg ttgacctggt agaccgcaaa caccggcggg aagaggaacg gaacgagaac
    15721 ggcgaagtca ccttcgcgat tccggaattc aagacgggtg atctggtgga cgtgcatgtt
    15781 cgaatcgtgg aaggtgacaa cgagcgcata caggtgttcc gcggaaccgt gattcagcgg
    15841 cacgggggat catccagcaa tgcttcgttc accgtacgca aagtgaccgc aggcatcggc
    15901 atcgaacgga ttttcccgct ccattcgccc cgcctcgcga agatcgaagt agtccgcagg
    15961 ggccgcgtga gacgttcccg tattttctat ctgagagatc tgcgtgggaa ggcggcacgg
    16021 gtaaccgaga aacgggacct gcgcaccgcc gagcagaaag ctgcggcagt cgagacggcg
    16081 cagtaagact tcggggaatt gacatggaac ggcgcagagg agcgtgtctc tcctctgcgc
    16141 cgtcatccgt ctgcaccttt cggaacgcac agattcagag gttcccccgg cgcgacaccg
    16201 ccctcgccaa tggcggcctc acacggtggt ggtacggatc atccgtttcc atctcgcagt
    16261 ggtaaatctc gatcagacgc tccaacgcac gttcggcctc ggaagttgcg aacccttcgc
    16321 ccgcgaatcg ctcatggtcg agaatcgcgc gcagacgcag aatctcccac cgccagcctg
    16381 tccgcgccca tgcgggcatt ttctcatgca ctttgtctgc gagttcccgt gcagttctca
    16441 ccaacccgac atccaccagc tgcttggtgt atgagcgtgt ggcagccatt tccagcgctt
    16501 ccacgagtgc aactgtctct tcaattgctt tgcctccgaa ttcgtaggcg atgtactctc
    16561 tgagagtgtc gcgtgcactc ctttctggcg tccagtagaa ctgcgccact atcgctttgt
    16621 tcatgtcttc gtagatgcct tcgctgtacg gaaatccacc cctcacaatg tgcttcacct
    16681 gatcccagag ccgctgaaaa cgcgcgggga gcgggttggc gccaacgccg ccccacggcc
    16741 agttgcccca catgctgatt tccgggaagt tgacgagagg tagattgccg ggcacaccga
    16801 catcgagagg gtatctcggg aagtcctcgt gtgcatctgc gaggatgtaa tcaacccagt
    16861 tgttaccgtc cttcagggca ttggtcaggc cctcccattc tccttccggt ggtgtatcaa
    16921 acatccacgt cgaaagaacg gtcttgatgt tcgggaagta ctcgcgcgcc agttcggcga
    16981 aatcgtgaga aaggcggaga aacccgttgc acccccaggg cgagcaccgg tcacacgcgc
    17041 acccgccctc gtcgtatggc cagaacgtca cgaggtccag gccgacgccc gacagctcct
    17101 cgaaaaggcg cctggcgttc gccaggagat agtcgcgtcc ctccgggtta ctcggacaaa
    17161 ccggatgccc gctgttgccc cggcgatgag tcgggtcggg caacggaacg gcgcgggtgt
    17221 gagaaggcgc gccgctgaac atcgtgttgt tgatgccgat cgcgaatttc agcccaaggt
    17281 cccgggcagt tctcgcgaat ttgcgcgtca ttgtgagggc atgttctgtt tccgggtcct
    17341 cccagtctcg cagattgatg aacgggaatg ccgtcttgat cgcgttcacg ccccagaggg
    17401 ccatgtcttc cacgtagcgg gtgatttcat tctccggggc gacgtggtac cagttgtgga
    17461 agtgagtggc gaagtacatt ccgcgcaagg aacctcgcgg cgcggacaca ccacgccaag
    17521 gtgaggccgt gaacgcaccc tcgaaccggc ttgtgcgcag gaatttgccc acgccgtaca
    17581 gcaggccgag aggcgagcca ccttcgatca ctgttgcccg accgtcttcg cgaatcctga
    17641 acgcctcggc ggcgaggctt ggctcgacgt tgagtaccag gcgcggattc tcgctgctct
    17701 gcgtgattgc ggtggtgtac cgttcgttga tccgcgccgt gagtatgcga aatacgcggt
    17761 tgacttgttc gctgtcgcga ggggtaaccc tgacgcgaat ttcgtgttct tcagccactc
    17821 caccgtctcc atcatctgaa ccgttttccc agaccctccc tgcgtacaga agggaaggaa
    17881 tcaacccgga gtagtcaagg agggacgctc cctcgttgcc ggcccgttcg ggaccggcga
    17941 tattcatttc agaagaaagg gacggcaact acgtgttgaa gccgtggcgt gtgcgggatc
    18001 cgaaatgacc cgcgtgacgc caacgaggtt tgcgcatgca gcagttctat gatcggtaca
    18061 ccgaagcccg tttggcggat ccccgccacg aatccgccag ccgttggaaa gccgctatgc
    18121 tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttgg cggatccccg
    18241 ccacgaatcc gccagccgtt ggaaagccgc tatgctggca gccacgtatc tcgaaaccgg
    18301 ggagttgtgc ggttcacacc tcaccatcgc ggaaatcggc ggggggttcg gaacggtggt
    18361 gaacgcgctg ggcctgatac tcggaagcga acggcttacg gtgtatgata tcagtgccaa
    18421 ggcgtgcgaa gaaggcaaaa aagcgtaccc aggcacgacg ttcatcaatg ccccgtacgg
    18481 agaaggaaat cgttcctcct gggatatcgt tgtgttatgt gatgtaaccg agcacgtgga
    18541 agacgaggat gccctgctgc gcaccgtcgc tgaggacgcc aggttcgtgt tgctcaaaat
    18601 cccgatcgaa aaatgcctct ggcgcgtgtg gcgacgactg aaggggaaac cgcccggttt
    18661 ttcgtggggg ccggaccacc aatcgggtca cttgagaggg tacacgcttt ggtccgcgaa
    18721 gaggcttgtg aggaggcatt tccacgtggt caacacgagt ctttcggaat cagtgtggtt
    18781 ctacggcagt gcaagaagtc tgcgcctggc gatacgtgtt ggaaaatacc tctcggtgat
    18841 gattttcggt ggatcgctct gcgttctcgc aagaccccgg gcccggtaac ggcggcacag
    18901 aatcagcata ccacgcgata ccccttcacc cactccgcca cgcgcttcac cgtctccggc
    18961 ctgcacgcct gcggcagcat tccagcggaa ctgacgacta tccccctgtg atgaggcaac
    19021 gcatcgagtt cggcttcgat tcgtgctatg atcgcttcgg ccggttcaag ccacagggca
    19081 gcgttcgtgc ccccgatcag gcacttgtcg ggacaggcgc gacgtccttc cgcgagcgtc
    19141 gtgttgccca ccgtgggcgt ggtaaacgcc tcgaatccgc tcaccgggat gcgtgccagg
    19201 tcaggtaaca gagccttaag gtgcccgcac atatgcagta tgaggtgtcg gcctgcgttg
    19261 cggacaatcc gcacgtactc gctgatatgg cgtgcgcaat accgtcggta ttgctccgga
    19321 gaaatcagtg ttgtggacgt gttctccgtc aggtagaaga catccgccgg gccatgttca
    19381 ctcaggattt cggtctttcg caccagatcc cggtggattg cctcgaacag gcgctcgacg
    19441 taggctggaa aatcgttgag caggtaatgg gcgttctgga ttccggcaag ccactccacc
    19501 cagaccataa gcggcgattc accgatgtcc tgagccgtta tggtccgctc accgacacgt
    19561 cgcgattgct ctcgcgcctc cgcgagttca ttcgggtcaa cttctgcggc ggaatcctcg
    19621 aagacgtcag ccagaatgtt gatttcatcg cgcgtcttga ccgggaatct gatgggatgc
    19681 caggaaaagg aggcgtggtc aatctgtgag acgagtgtga ggcattgacc gccaatacgg
    19741 tattctgttt ttcgaaaatc gccctcgatt acggtattca cggacgcccg tcggtgcacg
    19801 gtacgtatgc ccgtcctgag gtgaaccgtc ggatcactgc cgatccactc gtggagcgcg
    19861 tcgacggtca tccgggagaa gggagcaccg tgaaacccgg ggtagttgcc aggaagtttg
    19921 gcccagaagg gaaggcggtc caccggttcc atccgaacgg cggccttcca gcgctcgcgt
    19981 ggggtcattg tgtcggtcac gcggtaccct cctcgctggg aagcctccgg tgcgggcgac
    20041 gcccgcggga cacctcgaac ggggtcatgg cacggaaaaa cagcattgga cacgtgcggc
    20101 gcgtccagtt gccccggcgt cgctgccgtt cgcgcttcta ttcgtacctc agtgcctcga
    20161 taggatccag tttggccgca cgactggcgg gataaatccc gaaaaacaaa ccaacaccca
    20221 cggaaaaccc taccgcaagc aacagggact gcggcgttac gatgacttcc cagccgcccg
    20281 agctggccat cattttggca acccagtatc cgaggcccat gcctatggcg ccgccgagca
    20341 cacacaacgt gatggattcc agcagaaact ggaggagaat aatgctccgc cgggctccca
    20401 gagcctttcg aatacctatt tcccgggttc tctcggttac actgacgagc atgatgttca
    20461 tgatgccgat gccacccacg atgaggctta ccgcggcgat actggcaagc agcagtccaa
    20521 aaatcttggt gctttccgcg aacgttgttg ccagctcctg ctggttgaag aaacgaaagt
    20581 cgttctgctg ctccgggcgc agttggcgtt cgcggcggag gattctctcc atttcgccct
    20641 gcgctgccag catccattcc tgtgcgacaa cctgcaccgt cacgccgttg attcgatcgt
    20701 tgcccatgag tctgtactgc gccgtggtga tcgggatgaa gatgttttcg tccgggttgt
    20761 cccagcctcc ctgggagccc ttggattcca tgattcctat cacgtcgaaa atcatcccgc
    20821 cgatcctgac cgtcgaacct acgtcctggg gcccgatacc gaggttctct gccgccacag
    20881 atcccagaac cgcaactctc tgagatgccg acatgtcgcc atttgtgaaa aacctgcccg
    20941 ccgcaagctg gtaactccgc aggctgaaat actcgggggt ggttccggtg attcgaacgc
    21001 tgttgttctt gttgccgtac ttcacctggt attgcccgcc catgtcagga acgactgcgg
    21061 tggccagcgt catctcgttc cgcaacgcct gggcgtcgtc ggtagtcagc gaacggtacc
    21121 gggtgactcc gcgtatggtg gttctgtctg caaacacacg caactggttc actcccatgg
    21181 aggcgatacg atcctgcact gctttctgcg cacccgcgcc gatcgccatc atcgcgatga
    21241 cggcgcccac gccgataatg atgcccagca tcgtcaggaa cgcccgcccc ttgttcgcca
    21301 gcagcgcgcc aaaggctacc cggattatct ccaaaatcag catggtgcac gtcctctacc
    21361 gcgagcttct gccgggaatc actgagaatt gcttcaatcg gtcgcgcatc gcctgagatt
    21421 gcatcgccag cgctgccgag gggggaaggt ataccgtatc gccttctttg agtcccgatg
    21481 taatctcggt gtactcccag ttctgtatcc ccgtcttgac cagaaccact ttcgtcgtgg
    21541 cgctgtcggg cacgaagacc acgccgacac tccccgcggc catcacaccc ggcgcaggac
    21601 cctgggggat gtctcgagaa ctgaagtgca tgctgccacc tctgcttggc cttgcccggg
    21661 ccgtcgcacc cgacggacgc gcgccccgcg ccgacgattc agaaggtgcg ttcgccatgc
    21721 ccggaccggg gccaccgcgc ctgcccggac cgcgacgagt cgaatcatga tccatgaatt
    21781 tggccttgtt cgcgtccacg aaggcattca cgctgtccgg gttcagcccg agaaacattc
    21841 cgacgacgcc tgcgttctgc aacgttttga ttgcgtcatt cggcagcgac ggaacgcctg
    21901 ttttcttcat gatctggacg tcaacgctgg cattcattcc cggtcgcaat aatccttcgc
    21961 tgttgggaat ctgaacgatc atcgggaaaa acgtgacgtt ctgctcctcg actgattgtg
    22021 gttctatctt ctgaatttcc cctgtgaaat tcctgccggg gaacgcgtcc acggtgacgg
    22081 taaccgggag accgggcctc agtttcccca cgtcggtctc gtcaaccatc gcgcggacct
    22141 gcacgctgct gaggtcggcc atagtcatca gtgttgtccc ggcattcacc tgaccaacag
    22201 acgcgattat ctgccccttt tcgacgttct tcttcaggat cgttcccgtg gaaggtgcgc
    22261 ggacgatcgt ctctatcatc ctttccgttt tggtttcaag atccgtctcc gcgcgaatga
    22321 cgtttgcacg ggctgttgcg taatccagtc gggcctggtc gtattcctcg gttgacgtca
    22381 ttccccgtgc gagaagatcg gcggcccgat cgaggctttt tcgagatacg ctctcccgca
    22441 ctctcgccac ttcgagatca gcccgagcct gtcggtatgc tgcggcgacg tccgtcgtat
    22501 ccacctgcgc gagcagagcc ccggccgaaa cgacgtcccc ggtttcgact ggcagagcaa
    22561 gaatctctcc cgatgccttc gatttgatct cgattacgcg gatcggctcg attgatccgg
    22621 tggcggtgac cttaatctcg atatcgcgac gttccacttt cgtcgtggta aaaaggtcta
    22681 cagattcacc ttcttttgat ttgttgcagc cgggcattga acatccggcc attgaaacgg
    22741 cgagaaggaa caacactgct gctcggcggt tcacgattcc agtcatcctg ctgattcctt
    22801 tttattggcg tcgtctgact ctattttccc atcccgcagc acgatatgcc tccgggcatg
    22861 cgctgcaacg tcggcttcgt gcgtaacaag aacgattgtc tggccggaag catgcaactg
    22921 cgcgaacaat tccaggatgc tgttcccggt tgcagtgtcg aggttgccgg tgggctcatc
    22981 cgcgagaagg atgctggggt cggtcaccag agcgcgcgcc acagcgacgc gctgccgttg
    23041 ccctccggac atctgattcg gctggtgttt catccgatcc gccagaccga cacgctcgag
    23101 cgcagcggtc gcccggcgcc gtctctcccg agtgccaaac ccgccgtaga tcaacgggat
    23161 ctccacgttg tgcaacgcgg tcgcacgggg aagcagattg aacgtctgga acacgaaacc
    23221 gatctggcgg ttgcgcacgc gggcgagcgc cgcatcactc attgaggaca cgtcgctgcc
    23281 cgcgagcagg tatctccctg ccgttggcga atccagacag ccgatgaggt gcatcagcgt
    23341 agacttgccg gacccggagg gcccggtgag tgcgacgtat tctccttcgg agacgtcaag
    23401 atcaattcct cgcaatgcat tcacgcgctc ggaaccgagt ttgtacacct tctcgatccc
    23461 tcgcatttcg atcaacatca gcgtatgccc cttccaatgc ttcgccctgt ttcccgccgc
    23521 aggaatgcag ccgacactgt gccgccgagc ctgctgaata cctttcagtt aaacgaacgg
    23581 gaaggcggtt ttcatatctc caacatatga gattctcgag aatatcaccc ggtcagaacg
    23641 aatccgaggt gcatggtgcc gcggcagtga acaccaacgc gcggcttgac aagtgttccc
    23701 agggtgtcta caataaggca ccacgtcgat cgcccctttt caaaaggaac tattccaatg
    23761 ccgctgtccc aatggggaag aattctggtt gacgagatgg aggccgccgc agttccgccc
    23821 ggggacttgt ggatctggcc actgggggga gcttcgcttg cagtgaagtc cgctcgcagc
    23881 atggtataca ttgatccgta cacaggatca ccctccagcg gcgtctggat tcgaatggtg
    23941 gccgttccgt tcgatccgcg tgacgtgcgc atcgcggacg cggtgctgtc cacgcacgac
    24001 cacgatgatc actgccacgg agagacgttg cgcccgttcg cggacaacac gggggcgttg
    24061 ttcatcgggc ctgcgtcctc ggcgtccaaa atgcgctcgt tcaaacttcc cgaaacacgg
    24121 atcatgagcg cccgccatgg tgattccttc cgtttctcag atataaccgt agaagcagtc
    24181 gagatcgtgg atcacagcga cccgacgtcc gtggggtggc tggtatccgt ggacgacgga
    24241 ccagtgctct tcgacggcgg cgacgggatg tacgacgact acttcaagaa ggttgcggag
    24301 acgcgccgtg tctctgcggc aaccttgagt attgcgggcc tcactggcga cggaagaaaa
    24361 atttacatgg acgcgcggga actcgttgcc gccgcagaag atctcggagt tgacctcctc
    24421 atacccaaac actgggatct ctggcggaac gtgtggctgg atccctggga ggttgtgatt
    24481 gccgcacagg cacgccgcgc tcggtttcgc gtccacatcc cccgcctggg agaagtcatt
    24541 cggctcccgg cccgctgaag catcggacga agcagtctgg aggaatccca ggtggcaatc
    24601 tctctccgtt gcggagatgc ccggcgcctg tggcttactg tttttagaga aatacgcgat
    24661 attctggtag cgagtttctt actcaacgcg cttccctggc gaagtcttca cgtacgtctc
    24721 ttcagacatc acctggattg gagggtccca tgatgcgccg ttttcttgca gcttctctgt
    24781 tgttagccgt tgctcttcca gcagttgcag ccgtctctgt cgggcagaag ggttccgatt
    24841 tttccctctc gaacgtagat gatgttcgct tctctcttgc gacgccgcga actacgccgc
    24901 tcgttctgat attcaccacg aaggatctgg gaaatgtgag ccagccgtgg agagacagtc
    24961 tgacgacgac gttggggagt tccgcgcaga ttcagactgt acttgatctc ggggatgtgt
    25021 cccgcttcct gcacagcgtt gctcgcacgc gtatcagggc gcagaaatcg aaagcgattc
    25081 tggactggga cggcta
//
LOCUS       DAOSOO010000022        24668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2041_length_24668_cov_5.259050, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000022 DAOSOO010000000
VERSION     DAOSOO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24668
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_24668_cov_5.259050"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1879)
                     /locus_tag="PLT86_02990"
     CDS             complement(149..1879)
                     /locus_tag="PLT86_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HOT35612.1"
                     /translation="MASSDSLLITPYGGTLVDLVIPSEEREHLAKYAGGLPSIQLTER
                     QLCDLELLATGAFSPLDRFMGAEDHARVLDEMRLSSGHLFPIPVTLSVDPSDAIHLDS
                     DIALRDAKNDLLAVMTVEEAYEWDRDEVSRKVFGTLDEKHPLVAEMRRWGKVNISGRL
                     RVIRLPRRYDFRELRLTPAQTRARLEAYGHANVVAFQTRNPLHRAHEELTKRAAESVD
                     GVLLLHPVVGMTKPGDVDHYTRVRTYKALAEHYYDPDRILLSLLPLAMRLAGPREAVW
                     HAIIRRNHGANHLIVGRDHAGPGNDSTGKPFYGPYDAQDLVQQHEKELGVKALPFSML
                     VYLPDEDRYEEDSKVPKNARTASISGTQVRDEYLNKGRALPSWFTRPEVAQILAESYP
                     PRDRQGFCVWFTGLSGAGKSTIADILAVRLLELGRQATVLDGDVVRTHLSKGLGFSKE
                     DRDANIRRIGFVASEIVRHGGCVIAAAVSPYRATRDEVRRMVGSDQFIEVFVNTPLET
                     CEQRDVKGMYAKARRGEIKGFTGIDDPYEPPVNPEVMLDTVACTPEMNTEQILDLLGR
                     RGFVSNGKKE"
     gene            2307..5087
                     /locus_tag="PLT86_02995"
     CDS             2307..5087
                     /locus_tag="PLT86_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35613.1"
                     /translation="MPEFSISTQFVRYTVGTDAMNRAFTDVNTDTNYAIPAPCASIIK
                     GGVEFRATEAWEADGLLHLAFGGSGVKARLMAHAEKHHIVLRVVAVDGEGVEEFTFAH
                     IPLRLAGTLDDPFGASALALNLKTNVRELPGPMAQLCATCYAKFGFAGASVALVATPM
                     DTLRKGMQEAVSAAPELPQSRVGGPWAMDAKITRGSYLFNFGDMSEEKADDWIALAQS
                     LGITQIDFHGGNSFRFGDCRPNPVTYPNGFASLKAVIDKLHAAGISAGLHTYAFFIDK
                     TCPWVTPVPDSRLAKDRILTLAAPIAADADTITVKEDTSGMSAITGFFVRNSNTLHIG
                     DELITYTGVSKEAPYGFTGCTRGACGTAPAAHPAGAAVGHLKECFGLFVPDPETTLFE
                     EVAAKATEAYNTCGFDMSYLDALDGEDILGGAEWAWHYGSRYVFEVAKRLKKPALFEM
                     STFHHHLWYVRARIGAWDHPGRSHKKFIDLHVASNEQGRRMFLPAHLGWWAVKTWHGH
                     QTEPTFSDDIEYLCCKAVGTDTGFSVMGINPENRDKPVFQRLGAIMRRYEALRHSGTV
                     PDAVKARLKEPGAEFTLVDTADGKSAFLPTQYDKHRVEGADGWSDSWTVNNPYAAQPV
                     RLRIEALLSAEPYDSPNAITIADFTDPARFSDRAAYDGITLDLAPAEGGALFSAHNSN
                     PPKPSAKKELRYSPTEHGVRVTQKADPSWAKAGATFDPPLNIDKHEALGLWIEGDGKG
                     EVLNLQLKCPEHLVTGIGDHYVPITFTGRRFVTLIEPEGARADEYSWPYDGNVYALYR
                     EGIDYTQIRSVSLWFNAIPAGESVSCRLGPIKALPMRPVTLRNPSVIMGGQEITFPVG
                     MKSGWYIEYAAGEPCILYDDQGTPVAEVTPRGAAPVLQHGENSVRFVCETDEHLPARA
                     HVSVITRGEAIG"
     gene            complement(5306..6790)
                     /locus_tag="PLT86_03000"
     CDS             complement(5306..6790)
                     /locus_tag="PLT86_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35614.1"
                     /translation="MKHLFIDDFHVEEINNLARKLHQPEKFPRNVAIRPEYRWENCGI
                     QIRTTPAWDPEAGRFTMIYLASGESLDAEVKIGVTGAPEGGTAFYCYATSEDGINWEK
                     PFLGLHDYEALTWNGERMGTRNNILPSAQGMLLGPIYDPHDPDPARRFKGLAYRGGHL
                     EPLFSPDALHWTVSGTPPVLSSDESHLTLDEKKKLFIATVKHGGPYGRSFYLTTSEDF
                     QNWSKQELVFHADQTDQENGFERLRKLIADPAYLKIVHNRPEEWRTDIYNFPVFPYED
                     LYLALPVMHHWSGKHVPMYENVDSRKSVELASSRDLRTWHRVANRAPFLEHSPVGDGS
                     AYDTGQIVTTNGPVVRNNELWFYYVGLRHRCISIEDTLNRKYLDSSAVCMARLRMDGF
                     VSLKGGVEWGSVLTRPVNVTGDHLHINIDSWRGRVLVEMLDADTMQPVPGFGKDECIP
                     TIVNSIDATVRWKGNANLASLQGKTVRLRFHLWQAEMYAFWFGG"
     gene            complement(6808..8931)
                     /locus_tag="PLT86_03005"
     CDS             complement(6808..8931)
                     /locus_tag="PLT86_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35615.1"
                     /translation="MKQTAVSEVLERLGEPFAAGFMRDPDATMVERFANALNTWARVL
                     PLRPYDGGLLYPSGPKYWNPEGMAVGWYYVTATPLDRGVFEKKLAASNPEEREALAEL
                     ERVWSAHVFPGGYTHSIPHYERVLREGLNGFDERVRKGLQTALSERDSRAGFFRAMET
                     TLDTARILHRRIVEEIERFVPGDDEQRGNRDALLAALRVVPFRPAERFFDALVATNFT
                     FYFDGCDDLGRFDQYLRPYFERSLAEGELSHDRAVELVRAQWENVNAATAWNAAIGGT
                     KPDGTDLSSELTIVCIEAARGMRRPNLALRLGPSTPDSVWEAALDTIVSGCGLPALYW
                     DPNYYRAMDASGIPLPVKERHEYAFGGCTELMVQGRSNVGSLDDDINVPGVLERSLHG
                     RLPTAADFPEFREGFRNDLAATIASIVERVNLWQQQKAEWQPQPIRSLLIDDCVDRGI
                     EYSAGGARYNWSVINVMGLANAIDSLAALRTLVFEERRFTGREIVDVLARDFDGAEEL
                     RRELENAPRYGNGDPEVDSLAHDLSQFIFTEFLKYRTWRRGADGEGGPFVPSCLMFVT
                     YVPFGRAVGATPDGRRAKEPIADSAGPYQGRDRSGPTAMMRSVTQLDQQHAPGTLVVN
                     LRLARHHFATPEQRANTKALLRTYFDMGGLQIQVNVVDQKVLRDAVAHPERYGDLIVR
                     VGGYSEYWSNLEPALRESVLLRTEH"
     gene            complement(9114..9587)
                     /locus_tag="PLT86_03010"
     CDS             complement(9114..9587)
                     /locus_tag="PLT86_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35616.1"
                     /translation="MSNQFWTWFVVATAILVAAILRPRATRIFLGFFFIAMGLAVNLT
                     LALTDPQSFVGLGSHSYLPLYRWVFENLVARNPVLMVAPVIVYEVSIGTLMLGKGTAA
                     RLGFAGAIVFLLAITPLNAECLPNPILALAAARLWRIQWERDLLDMLRDWRESRC"
     gene            complement(9600..10796)
                     /locus_tag="PLT86_03015"
     CDS             complement(9600..10796)
                     /locus_tag="PLT86_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35617.1"
                     /translation="MSTNRHEDPKAWFMQARFGMFIHWGVYAIPGRGEWVMYREHIPA
                     EEYAKLADQFNPKHYKPRDWVALAQDAGMRYMVLTARHHDGFCLWDTKTTDFSAPRTA
                     AKRDLLAEYVDACHSMGMRMGFYYSLADWRFPGGLYCYPRDPAIDYEPMVAQAHSQIR
                     EPLTNYGKVDMLWYDGGHPGDIWRGAEMDAMVRALQPEIVTNGGPGTAGDFVTHEQNT
                     DGDPRPWEACVTMNDCWGYVPSEKGYKTVMQLIGTLVTCASKGGNLLLNVGPDPEGRI
                     PAAAVDRLRSIGKWMRVNGEAIYGSTRTVLRPPSEVGNATQVGNRQFLHIHAWQGSTL
                     AMAWVGNRVTRARVLATGTEAQIEQKGDRVWLHGLPQYAPDPDISVIELEVEGEPRYP
                     ELRFHF"
     gene            complement(10826..12583)
                     /locus_tag="PLT86_03020"
     CDS             complement(10826..12583)
                     /locus_tag="PLT86_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35618.1"
                     /translation="MRSLPQDQRDSTTENDGGGLTRREFIALGASAALGMVVTATRGW
                     ARPAREARMADNRTGTRMRLHEAYWINGVPVLGAFNVRSLEDLQIAHDAGFNMAAYAH
                     LDMLDPDTPMGHYALDNGLKAMHSITGPTHGGPRLSRAIDAREAIIPFESGAKPAPGP
                     GVVVIEDERIRYRDSTDTTLVGCERGADGTEAKPHRALIIMFWPEPFALEIAKVKDSP
                     NLWGYWALDDTPGYALSAMRGLTSVVHEVDGHHPVCGGYSGPDTMFNFGPGACDIIVP
                     YWYPVLKWGYDRQMTSMDTQAILVYARRQVPGIPFMGLYQAFWGADWNKRGPLTAFEI
                     REQAEDFVREGAAGLMGFAVLHQTNPNDIFGGFNLDPEMTRAVREINDEVRRTCSLTV
                     PPERPEMAAIRIRPIGFQAREPEPQGIPPAWRIAFPFDAGESGDLDTNLAPDAGIDLT
                     ATYAGKDGEQASWETIGTRGGVIAFLDLIGPVKKAENTIGYAVCTVTSAVERTVQVRL
                     GAAKDTLVRVNGKEIWRYTGSRGIHLDDNVAWTTLPAGRSEIVVKCYNRGGGWGFALR
                     LADPDGRPAAGLSFEPAAG"
     gene            complement(13042..13983)
                     /locus_tag="PLT86_03025"
     CDS             complement(13042..13983)
                     /locus_tag="PLT86_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35619.1"
                     /translation="MTRREIVKAAIAHKETERVPYCIGFTGGGEKRLQEAIGRQTAQE
                     YVDNDVVRIGVPWWNWHELGPEWRAADPPSTPATVIGIGSYPQFFESIRMLRDNCDKY
                     ILAMIYGSHFEKAYFTRGIENFLADMAGYPEFARKLLNRIIEKNMVMLENFLMAPEID
                     GVLLGSDWGSQVDLLMSPDTWQDMIRAGEQKEYDLVHSYGKDVWVHSCGCVERIIPSL
                     VEMGLDVLNPVQPECMDLAALKRDYGDKLAFWGGISTQQVLPFGTPEEVKAEVRRVRK
                     LMAANGGYILAPAQEIQEDVPAENIIALVEAAREPGV"
     gene            complement(14022..16259)
                     /locus_tag="PLT86_03030"
     CDS             complement(14022..16259)
                     /locus_tag="PLT86_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35620.1"
                     /translation="MGFREERTAFEREFEACYSTLPNDVTVMRSRLDAESAANPEWTP
                     YRLKTLLYETIARECPVKVFRHFPFFYEIGVGKQRTDLGAGGIGAWLKEQPFGRELAA
                     VGSAFWRPYSECGLSHGWAVLDDNHHSLGYDNVFRAGLTGLVAEARARLERGCSTEEQ
                     AFLEAMIAGLSAQMHLATRFADEARGMLLVETDPAVKARLARIAATAERVPAFPPETF
                     FEALATILFMRETTQALEGNGISVYGHLDRILIPFYENDIAESRLTRDEAKDLLAFFL
                     ARCDAAFGMREARNHVGTNTTVVIGGCDVNGVPVFNEITRMILDVYREHALVDPKLNA
                     RVSSGHQEEFFRLLAEFSSTGSNVLSIFNDDVIIPANARMGKAVRDCRLYVGGGCQEN
                     VLENTEINSRATMYMSLPGVLLMGFFPERWDELARRDGFVIVPLDAARTSEEFYAAFL
                     RNLRAVIGAHVAARNRTEAEGTRYNPCPLHSATISDCIERARDMMAGGARYSFGSVSL
                     AGISTLIDSLYAVKMVVFEQKRLSLARFRDILAADFEGEEAFRQYIINRIPKFGHGDP
                     GMREFSARVFADVARAASGYPNTRGGTYEASLFSFRSFADFGRRGGATPDGRHAGEHL
                     SPGMSPSQLALGRECNVTQVLRALEPVDMTDYPVVAVLDVKMPLSPEQADPSVLESVV
                     RCFVDYGGSVLQINVVDPSALVDAREHPERHPDLVVRVSGYSAYFRTLSPDIQQEVVA
                     RALAR"
     gene            complement(16373..17221)
                     /locus_tag="PLT86_03035"
     CDS             complement(16373..17221)
                     /locus_tag="PLT86_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35621.1"
                     /translation="MFPNLSPGAVGIRANIEEAATLARTNGFKGIDIDIHEPERYGAE
                     KLKALLKENGLRIGGWGLPVDFGGSDEKFREGLKELPKWAAFLRQFGCNSFMSWMMSG
                     SNEFTFAEHFERMRSRFREIGLILKDHGCVFGIEFLGPKTIRANFKHEFIHTLPQMIE
                     LCDAVGTGNMGVLLDSWHWFTSGGNLAQIRKLRPEQVVYVHINDAPKGVALDKHIDDQ
                     RAMPMETGVIDLPDFLRALNEIGYTGPVTPEPFNKALAQMPAEEAARVTAASVKKAWE
                     AAGLGW"
     gene            complement(17276..18118)
                     /locus_tag="PLT86_03040"
     CDS             complement(17276..18118)
                     /locus_tag="PLT86_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOT35622.1"
                     /translation="MQHGESNPRIEPKRRAPWRDKWYRIIFESDTPAGRLFDVVLMVA
                     IVLSVLTVFVESVRSIRFRFGSVLFGLEWAFTLLFSVEFALRIATAKRPMRYVFSFFG
                     LVDILSILPTYLSLALPGSQYFLSVRSLRLLRAFRVFKLSKFLREANVLSTALLASGR
                     KITVFLSTVSMLVVVIGSVMYMIEGEENGFTDIPTSIYWAIVTLTTVGYGDVSPHTPL
                     GKTLASLVMILGYSIIAVPTGIVTVEMSQAKKRADAEKSCPQCGKTGHDLDARFCKHC
                     GAAL"
     gene            complement(18125..19960)
                     /locus_tag="PLT86_03045"
     CDS             complement(18125..19960)
                     /locus_tag="PLT86_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT35623.1"
                     /translation="MKPLAFAVGLQAAFSGGNLQSSSAVALAISICAVVCLLLLSILV
                     LSFGGRVRRFLAQTLVSKLLAEHPGEGRATPLWHRAVRATVETALWLVNVCASLALFY
                     FAASMALDLFDLQDEGLVYLQGSLISLLGLVVFFRINGSFPGVYRRLENWRSPLTIRG
                     HKLAEVETVADFLTFSSKALRVSFALVSFYVVGRYFTSLFPAGEAQASYEVVRGVMYA
                     VLATVLLAVVVRAINRFGAAVAARSRDWAAELEASPEETTLYVKMVHLGHTGIQILRV
                     MLLVLAVYGYAAFVLGLFAVTSTWVGSIVEMLVTPVRQVGDTVVGYLPNLFAIIVILA
                     VTYAIVKAVRWTASEIGKGTVTLPGFFPEWAAPTYKIVRFLIIVFCAVVLFPYLPGSG
                     SPAFQGISIFVGVLFSLGSSGAVSNIVSGVVLTYTRAFRVGDMVKISDTTGTVVEKTL
                     LVTRVRTVKNVDITIPNSMVLGSHITNFSSSGNTLIVHTSVTIGYDVPWRVVHELLLE
                     AADRSSSLLKNPPPFVHQTSLEDFYVEYELNAFTALPNTMAATYTELHQNIQDVFNER
                     GVEIMSPHYRAVRDGNQVAIPAEHVPEDYEAPRLRVETTQPHKKA"
     gene            20214..21029
                     /locus_tag="PLT86_03050"
     CDS             20214..21029
                     /locus_tag="PLT86_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT35624.1"
                     /translation="MNGEQVTFSVFTKPWKTLSLGELGALVRGFGFDAVELPVRPGYQ
                     VQPDTMETGLKEAVRAFADEGIVVKSVAAPTDGVRPGFIGELIAACAENDIPVIRVFP
                     FIRPDEPYLEGEARVQNELESLLPALEQCGVTVGVQNHCGPCFTGAMHLFHLIERFDP
                     QHIGAILDAGHTGLAGEEPEHAIDTVWSHLCMVNFKNAFRRLRTGPEAEQAEWQVYWT
                     SARHGLSDWRRYAAELKKRRWRGTACLTAEYSDPNAVDRLIADDLRYTKSLFV"
     gene            21059..22519
                     /locus_tag="PLT86_03055"
     CDS             21059..22519
                     /locus_tag="PLT86_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT35625.1"
                     /translation="MGTDRPNIVFFLADDQGAWALGCAGNREIRTPNLDRLAAEGIRF
                     TNFFCASPVCSPARASILTGRISSQHGIHDWIRAGNVPNEVPGVTHGRPVEYLRGMIG
                     YTDVLAENGYTCGLSGKWHLGDSLRPQKGFSFWNVHARGGSGYYDAPMIRDGIAYTEP
                     RYVTDVIAENAVAFLREQQATGTPFYLSVHYTAPHSPWTPDQHPRRTYDEYYQRYPFN
                     STPFEPMHPWQISSAPTGFDELSRRRELSGYYTAITEMDRTIGDVIAELERQGIRQNT
                     LVVFTSDNGMNMGHHGLYGKGNATFPANMFDTSVKVPMIVSRPGSVPSGVVCDEMLSH
                     YDLLPTFLDYVNAENPVAGETPGVSFVPILRGDEFARRDAVVVFDEYGPTRMIRTKEW
                     KYVHRYPYGPHELYDLVSDPEERDNVVEDARCQNVLRDLRARLSEWFVQWADPARDGS
                     REPVRGVGQHDLAGPAGAGVVAFNQDFRFLSTSHGQ"
     gene            22595..23704
                     /locus_tag="PLT86_03060"
     CDS             22595..23704
                     /locus_tag="PLT86_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35626.1"
                     /translation="MFNAFPRDETITRRERFCRLYDFEPVDSHVRWEAVAFWGDTARD
                     WVAEGGLPEDADPMDYYGMDPRNGISGNLGFTSMGLSGPRPETHVIAEEGRTRVYRND
                     LGQVWRDRVDGVSMPQWIRFPVESRDDWERIIKPRLKPEDHDFGNLDEEAVRSRTSQD
                     AWGLWIVGLYAFWRNFWGEEKLAFAFYDQPDVLHDMAKCWLTMHCECTPRVLDTARID
                     YVMFHEDMAFRNGPLIGPQLFDSFMAPYYRELFVHLRNRGQHRFLLDSDGNNGPILER
                     FADLGMNGLYPFEAAAGCDAIAFRKEHPRFFVWGAIDKRVLLGNKDDVEREVMAKVPA
                     LWAGGGFIPSIDHSVPPCSQENFEHFINLVRSICR"
     gene            23737..>24668
                     /locus_tag="PLT86_03065"
     CDS             23737..>24668
                     /locus_tag="PLT86_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35627.1"
                     /translation="MAIRKLEPVRVNPVLRHPDEQRVSLDGRWLFRLDPEDIGVRERW
                     FRDPAVLDASVQVPGCWQGQGHGGDGNDRVWDFGLEERTFRATYKGTGWYGRAFTPPD
                     EWQGRRVQVLFGGVHPSAEVWLNGERLGENGMPFVPFGFDITSNLRFCSENLLVVRVH
                     EAARIYGLAYSWQGNWSGLYRGVELTATGSTRIARCSAYPEVAAEAMRVAIELDAVAP
                     RGAKIRASVIAIGGNSAPITVEAPAKGKNIEFRVPVPSPRLWSPETPGLYRVDIALVV
                     GSVVIDALSERTGFVQLSTRKNQFLINGEPYYMRG"
BASE COUNT         4785 a         7834 c         7231 g         4818 t
ORIGIN      
        1 tctgcatcgc acgtatcccg tggcgcgcgg ggtcattctg acggcgttgc cggtcactaa
       61 cccgccgaaa gggccgaaat tccactcgcg cgggtatgca tgccatggtg gcaacatcgt
      121 agaacaggga gcccggatat tccgttgttt actctttctt gccgttcgaa acgaagccgc
      181 gtcggccgag caaatccaga atctgctcgg tattcatttc cggcgtgcag gcgacggtgt
      241 ccaacattac ctccggattg accggcggct cgtacggatc gtcaattccg gtgaacccct
      301 tgatctcacc tcgccgcgct tttgcgtaca tccccttcac gtccctctgc tcacacgtct
      361 ccagaggcgt gttgacgaat acttcaatga actggtcgct tccaaccatg cgccgtacct
      421 cgtcgcgtgt agcccggtac gggctcacgg ccgcggcgat aacacatccc ccgtggcgca
      481 caatctccga cgcgacgaat ccgatgcgtc gaatgttcgc atccctgtct tccttgctga
      541 atccgagacc cttcgagagg tgtgtccgca cgacatcccc gtccagtaca gttgcctgcc
      601 ttccaagttc cagcagacgc actgcgagga tatccgcgat ggttgatttg ccggccccgc
      661 tcaaaccggt gaaccataca cagaacccct gccggtcccg gggaggatag gactccgcaa
      721 gaatctgcgc gacctcggga cgcgtgaacc acgaaggcag cgcgcgcccc ttgttcaggt
      781 attcgtcccg gacctgagtc cccgaaatcg acgccgtgcg ggcgttcttc ggaactttgg
      841 aatcctcttc gtaccggtcc tcgtcaggca gatacaccag catgctgaag ggaagcgctt
      901 tgacgccaag ctccttctcg tgctgctgga cgagatcctg tgcgtcatac ggtccgtaaa
      961 agggcttgcc cgtcgagtcg ttgccgggtc cggcgtggtc gcgtcccacg ataagatgat
     1021 tcgcgccatg gtttctgcgg atgattgcgt gccagacggc ttccctcggc cccgcgagac
     1081 gcattgcgag aggtagaagc gaaaggagaa ttctgtccgg atcgtaatag tgctccgcca
     1141 gcgccttgta cgtgcgaacc cgagtgtaat ggtccacgtc gccgggcttg gtcatgccga
     1201 ccacggggtg aagcaggaga accccgtcca cgctttctgc cgcgcgcttc gtaagttcct
     1261 cgtgcgccct gtggagcgga tttcgcgtct ggaacgcgac gacgttggca tgtccgtacg
     1321 cctccagccg ggccctcgtc tgcgctgggg tgagccgcag ttcccggaag tcatagcgac
     1381 ggggaagccg aatcacgcga agtctgcccg atatgttgac cttgccccac cggcgcattt
     1441 cggctaccag cggatgcttc tcatccagcg ttccgaacac ctttctcgaa acctcatccc
     1501 ggtcccattc gtacgcttcc tctaccgtca ttaccgccag cagatcgttc ttggcgtcac
     1561 gcaaggcgat gtcgctgtcg aggtggatgg catcacttgg atcgacagaa agcgtcaccg
     1621 ggatggggaa caggtgtccc gaagagagcc gcatttcgtc gagaacccgc gcatggtctt
     1681 ctgcgcccat gaaccgatca agcggcgaga atgcgccggt cgccagcaat tccaggtcgc
     1741 acagttgccg ttcagtcagt tgaatggatg gaaggccccc ggcgtatttc gcgaggtgct
     1801 ccctctcctc cgaggggatt acaaggtcca cgagtgtgcc gccgtacggc gtgatgagca
     1861 gggaatcact tgaagccatc aattcagtag tctccgtgag ggtgcgtccg cagtatgctc
     1921 aggcagtaac tgagagggta tccttattac tcgagggcaa aacgcggctc aaccgccgcg
     1981 atatccccca cgtgttccag aaatcgggga aacccgcctg tttgttctca tagaaggtga
     2041 ggccagaacc agatgatagt gccgggtacg ggcgttcctg aggcaaaaga ggggcctggc
     2101 gagtattgca tggatgatcg gaaaacgcgt agtcggcgtg gaattggcga aagactctgg
     2161 cttcgaacgt tattaagata cgcgacaccg caccagaagg gcaacccgga gccatctaac
     2221 ccccgcgctt gctcttcctc ctcctccggg cgttattcga aacagcgcgc acgctgttcc
     2281 acccgccatt gagggatgat ccttctatgc cagaattctc catttccacg cagtttgtcc
     2341 gctacaccgt cggtacagac gcaatgaacc gggccttcac ggatgtaaat accgatacga
     2401 attacgccat ccccgcgcca tgtgccagca tcatcaaggg tggcgtcgag ttccgggcga
     2461 ccgaagcgtg ggaggccgat ggcctgttgc acctcgcatt tggtggttcg ggggtgaaag
     2521 cccgcctcat ggcacacgcg gagaagcacc acatcgtcct gcgtgtcgtt gccgtcgacg
     2581 gcgaaggtgt cgaagaattc accttcgcgc acatcccgtt gcgccttgca gggacactcg
     2641 acgatccatt tggcgccagt gcgctcgctc tgaatctgaa aacgaacgtg cgcgaactgc
     2701 ccggccccat ggcgcagctc tgcgcgacct gttacgccaa attcggtttc gccggtgcgt
     2761 ccgttgccct cgttgcgacc ccgatggata ccctgcgcaa aggcatgcag gaagccgtca
     2821 gtgccgcccc cgaactcccc caatcgcgtg tcggcggccc gtgggcaatg gatgcgaaaa
     2881 ttacgcgggg ctcctacctc ttcaatttcg gcgatatgtc ggaagaaaaa gccgacgact
     2941 ggatagctct ggcacagtcg ctcgggatca cccagatcga cttccacggc ggcaacagct
     3001 tccgcttcgg cgattgccgg cccaatcccg tcacctaccc caacggcttc gccagcctga
     3061 aagccgtgat cgataagctc catgccgccg gcatctccgc cgggctccac acctacgcct
     3121 tcttcatcga caaaacctgc ccatgggtga ctccggtgcc cgattcccgc ctcgccaagg
     3181 accgcatcct gacgctggcc gcgccgatcg ccgccgatgc cgatacgatc accgtgaaag
     3241 aggacacctc cggcatgtcg gccatcaccg gctttttcgt ccgcaacagc aacacgctgc
     3301 acattggcga cgaactcatc acctacaccg gggtctccaa agaggcaccc tacgggttta
     3361 ccggctgcac tcgcggtgcc tgcggaacgg caccggccgc ccatccagcc ggggccgcag
     3421 ttggccacct gaaggaatgt ttcggtctgt tcgtcccgga tcccgaaaca acgctgttcg
     3481 aggaagttgc cgccaaagct accgaagcgt acaacacctg tggctttgac atgagctacc
     3541 tcgacgcgct cgacggcgag gacattctgg gcggcgccga gtgggcgtgg cattacggct
     3601 cgcgctacgt cttcgaagtg gcaaagcgcc tcaagaagcc cgcactgttc gaaatgagca
     3661 cgttccacca ccacctgtgg tacgtccgcg cgcgcattgg tgcgtgggac caccccggac
     3721 gcagccacaa gaagttcatc gatctccacg tcgcctcgaa cgagcagggt cgccgtatgt
     3781 tcctccccgc gcacctcggc tggtgggcgg tcaagacgtg gcacggtcac cagacggagc
     3841 cgaccttctc cgacgacatc gaatacctct gttgcaaggc cgtcggcacg gataccggct
     3901 tctcggtgat ggggatcaac cccgaaaacc gcgacaaacc ggtattccag cggcttggtg
     3961 cgatcatgcg gcgctacgaa gctctgcggc attccggcac ggttccggat gccgtcaaag
     4021 cccgcctcaa ggagcccggc gccgaattca cgctggtgga tacagccgac gggaaatccg
     4081 cgttcctgcc cacgcagtac gacaaacacc gtgtggaggg tgcggacggc tggagcgact
     4141 cctggaccgt aaacaacccg tacgccgcgc agccggtgcg ccttcgcatc gaagcactgc
     4201 tctccgccga gccgtacgat tcgcccaacg ccataaccat tgcggatttc accgatccgg
     4261 cccgcttctc cgatcgcgcg gcctatgacg gcattaccct agatctcgca ccggcggaag
     4321 gcggagccct gttctccgcg cacaattcca acccgccgaa gccctccgca aaaaaagagc
     4381 ttcgttactc accgacggag cacggcgttc gggtgacgca gaaggccgat ccctcatggg
     4441 caaaggccgg cgcgacgttc gacccgccgc tcaacatcga caagcacgaa gcgctcggcc
     4501 tctggatcga gggcgatgga aagggcgagg tgctgaacct gcagttgaag tgcccggaac
     4561 accttgtgac ggggatcggc gaccactacg ttcccattac cttcaccggg cggcggttcg
     4621 tgactctcat cgagcccgaa ggagcacgcg cggatgagta ttcatggccg tacgacggga
     4681 acgtgtacgc gctttaccgc gaaggtatcg actacaccca gatccgttcg gtgagcctct
     4741 ggttcaacgc catacccgcg ggggaatcgg tgtcgtgccg gctcggtccc atcaaggccc
     4801 tcccgatgcg gccggtgaca ctgcgcaacc cgtccgtgat aatgggcggt caggaaatca
     4861 cgttcccggt gggcatgaaa agtgggtggt acatcgagta cgccgcaggt gagccgtgca
     4921 tcctctacga cgatcagggc accccggtcg ccgaggtgac gccgcgcgga gccgcccctg
     4981 ttctccaaca cggggaaaac tccgtgcgct tcgtctgcga aaccgacgag caccttcccg
     5041 cccgcgcgca tgtatccgtg atcactcgtg gcgaggcgat agggtagcaa ggaattgtgc
     5101 gagcacaacg aggggcggac ctgcaacgcg gcgttgcttc ggtgtccgcc catggtccgc
     5161 ggaggtaggg cgccctcggc gagggcgccg cttcgccccg ctcccgaggg tgggacaggc
     5221 attcttgcct gccctgattg ctctgcgtga acgggagccg attctatagc accggacaac
     5281 tctgttccgc cgcggccact ccccttcatc ccccaaacca gaacgcgtac atctccgctt
     5341 gccagaggtg gaaccgcagc cgcaccgttt tcccctgcaa cgaggcaagg tttgcgttgc
     5401 ccttccagcg caccgtcgcg tcaatgctgt tcacgatcgt ggggatgcac tcgtctttcc
     5461 cgaatcccgg caccggctgc atcgtgtcgg cgtccagcat ctccaccagc acccgtccgc
     5521 gccatgagtc gatgttgatg tgcagatggt cccccgtcac gttcaccggc ctcgtcagca
     5581 cggatcccca ctcgacgccg cctttgagcg agacgaagcc atccatccgg agcctcgcca
     5641 tacacacagc gctcgaatcg aggtatttgc ggttcagcgt gtcctcgatg gaaatgcagc
     5701 gatgccgcag gccgacgtag tagaaccaga gctcgttgtt ccgcaccacc ggcccgttcg
     5761 ttgtcacgat ctgcccggtg tcgtacgcgc ttccatcgcc cacgggtgaa tgctcgagga
     5821 acggagcgcg gttcgccacg cgatgccacg tgcgcaggtc gcggctggaa gccagttcta
     5881 ccgatttcct actgtccacg ttttcataca tcggcacatg tttccccgac cagtggtgca
     5941 tcaccggcag cgcgagatac aggtcctcgt acgggaagac ggggaagttg taaatatccg
     6001 tccgccattc ctccggccgg ttatggacaa tcttcaggta tgccgggtcg gcaatcagtt
     6061 tccgcagccg ttcgaatccg ttttcctggt ccgtctggtc ggcgtggaac acaagctcct
     6121 gtttgctcca gttctggaaa tcctcgctcg tggtgaggta gaacgagcgc ccgtacggcc
     6181 ctccatgctt caccgttgcg ataaacagct tctttttctc atccagcgtg aggtgcgatt
     6241 catcggaact cagaaccggc ggcgttccgg aaaccgtcca gtgcaacgcg tcgggcgaaa
     6301 acagcggctc gaggtggccg ccacggtacg ccaggccctt gaaccgacgc gccgggtcgg
     6361 ggtcgtgcgg gtcgtagatc ggcccaagca acatcccctg cgccgatggc aggatgttgt
     6421 tccgggtgcc catcctctcg ccattccacg tgagcgcctc gtagtcatga agcccgagaa
     6481 acggcttttc ccagttgatc ccgtcctctg aggtggcata gcagtagaac gccgtgccac
     6541 cctcgggagc accggtcacg ccgatcttca cctcagcgtc cagcgactcc cccgaagcaa
     6601 ggtagatcat cgtgaaccga cctgcctccg ggtcccacgc cggcgtcgtg cgaatctgaa
     6661 tcccgcagtt ctcccagcgg tactccggcc ggattgcgac gttccgcggg aacttctccg
     6721 gctgatggag cttccgggcg aggttgttta tctcctccac gtgaaaatca tcaatgaaca
     6781 ggtgtttcat ggttccctcg cgtcggatca atgttcggta cgcaacaaca ccgattcccg
     6841 caacgcgggt tcgagattcg accagtattc cgaatacccg cccacacgga cgatcaggtc
     6901 gccgtacctc tccggatggg caaccgcgtc ccgcagcacc ttctggtcta cgacattcac
     6961 ctggatttgc agcccgccca tgtcgaagta cgtccgcaga agtgctttcg tgtttgccct
     7021 ttgttcgggc gttgcaaaat ggtgtcgtgc gagtctgaga ttcacgacca gcgtcccggg
     7081 cgcatgctgc tggtcgagtt gtgtcacgct ccgcatcatc gccgtcggtc cggagcgatc
     7141 ccgcccctga tacggccctg cggagtcggc aatcggctcc tttgcccggc ggccgtccgg
     7201 tgtagccccc actgccctcc cgaacggaac gtacgtcaca aacatcaggc acgacggcac
     7261 aaatggcccg ccttcaccgt ctgcacctcg ccgccacgtg cggtatttca gaaactccgt
     7321 gaagatgaac tgtgacagat cgtgcgcgag ggaatcaacc tcgggatcgc cattcccgta
     7381 tcgcggcgcg ttctcgagct cccgccgcag ctcctccgcg ccatcgaaat cccgcgcaag
     7441 cacgtcgacg atttcccggc cggtgaagcg gcgttcctcg aatacgagtg ttctgagcgc
     7501 tgcgagtgag tcgatcgcat tcgccaggcc cataacattg atcaccgacc agttgtaccg
     7561 ggcgccgcct gcggagtatt caatgccccg gtccacgcag tcatcaatca gaaggctccg
     7621 tataggctgc ggttgccatt ctgccttttg ctgttgccag aggttcaccc gttccacaat
     7681 gctggcgatg gtggcggcaa ggtcgttccg aaagccttcg cggaactcgg ggaaatccgc
     7741 cgcggtcggc agccgcccgt ggagagaccg ttcaagcacg ccgggcacgt tgatgtcgtc
     7801 gtccagcgat cccacgttcg accgaccctg caccatcaat tcagtgcagc cgccaaacgc
     7861 atactcatgc cgttccttta cgggtagggg aatccccgac gcatccatcg ccctgtagta
     7921 attcgggtcc cagtacaacg cgggaagtcc gcaaccgctc acgatcgtgt caagggccgc
     7981 ttcccacacg gagtccggcg tcgacgggcc cagccgcaac gcaaggttgg gtcgccgcat
     8041 cccgcgcgcg gcttcgatgc agactatggt gagttcgctg gaaaggtcgg tgccgtcggg
     8101 tttcgtaccg ccgatcgcgg cgttccacgc ggtggcggca ttcacattct cccactgagc
     8161 gcgcaccagt tccaccgcgc gatcatgcga taactcacct tcggccagcg agcgctcgaa
     8221 atacgggcgg agatactggt cgaatcgccc cagatcatcg cacccgtcga aataaaacgt
     8281 gaaattcgtc gccacgagcg cgtcgaaaaa ccgttccgcc ggcctgaaag gaacgacgcg
     8341 caaagccgcg agcagggcat cccgattgcc cctctgctca tcatctcccg gcacgaaccg
     8401 ctctatctct tcaacgattc tccggtggag tattcgcgcg gtgtccagcg tggtctccat
     8461 cgcacggaag aaccccgcgc gggagtccct ttcggacaag gctgtttgca ggccctttcg
     8521 aactcgttcg tcgaacccgt tcagcccttc ccgcagcact cgctcatagt gaggaatcga
     8581 atgcgtgtat ccacccggaa acacgtgcgc cgaccagacc cgttccagct ctgcaagggc
     8641 ttctcgttcc tcggggttgg atgccgcgag tttcttctcg aaaacccccc ggtcaagcgg
     8701 agtcgccgtc acgtagtacc agccgaccgc cattccctcc ggattccagt acttcggccc
     8761 cgacgggtac agcagcccgc cgtcgtaagg tcgcagcggc agtacacgcg cccaggtgtt
     8821 cagggcgttt gcgaaccgct ccaccatagt cgcatcggga tcgcgcatga acccggcggc
     8881 aaagggttcg ccgagccgtt caagcacctc cgaaacagcc gtttgtttca atcctcacgc
     8941 catcctctcc ggtaaaaagc gtatcccttc aatcagcgaa atccaacagg aatgtacacg
     9001 cttccctgcc accactcgca agcgcagagg accgtacggt gcagcgcaac tttcttccga
     9061 gcgcgatctg caggtgatgt ttcacatgtc cgccgcagca gtagcagtag gtctcaacat
     9121 cggctttccc gccagtcacg gagcatgtcc agcaggtccc tctcccactg aatccgccac
     9181 agacgcgccg cggcaagcgc cagaatcggg ttcggcaggc attccgcatt cagcggggtg
     9241 atggcaagca ggaacacgat tgccccggca aaccccagcc gcgcagccgt tcccttcccc
     9301 agcatcaacg ttccgatgct tacctcgtaa acgataaccg gtgccaccat cagtaccggg
     9361 ttccgcgcta cgaggttctc gaacacccac cggtagagcg gcagatacga gtgacttccc
     9421 agcccgacga acgactgcgg atcggtgagc gccagggtca ggttcaccgc cagccccatg
     9481 gcgatgaaga agaacccgag aaagatgcgc gttgcgcgcg gccgcaaaat tgccgccacg
     9541 aggatcgccg tcgcgacgac aaaccatgtc cagaactggt tgctcaccgt ggcgccgcct
     9601 caaaaatgga accggagttc cgggtacctc ggctcgccct caacctccag ctcaatgacc
     9661 gagatatccg gatcaggggc gtactgcggc agcccgtgca gccacacgcg gtcacccttt
     9721 tgctcgattt gggcttcggt tcccgttgcc agcacacgtg cccgggtcac ccggtttccc
     9781 acccacgcca tcgccagcgt cgagccctgc cacgcgtgga tgtgaaggaa ctgccggtta
     9841 ccgacctgcg ttgcgtttcc cacctcgctt ggcgggcgca gcaccgtccg cgtggagccg
     9901 tagatcgcct caccgttcac gcgcatccat ttcccgatcg atcgcaaccg gtccacagcc
     9961 gccgcgggaa tccgcccttc agggtcggga cccacgttca gcagcaggtt tccgcccttc
    10021 gacgcgcagg tgaccagggt gccgatgagt tgcatcaccg tcttgtagcc tttttcgctc
    10081 ggaacgtatc cccagcaatc gttcatcgtc acacaggctt cccatggccg cgggtcgccg
    10141 tcggtgttct gctcgtgcgt cacaaaatcc cccgccgtgc cggggccacc gttggtgacg
    10201 atctcaggct ggagtgccct gaccatggcg tccatttccg cgccgcgcca gatgtcgccg
    10261 gggtgaccgc catcgtacca cagcatgtct accttgccgt aatttgtcag cggctcgcgg
    10321 atctgcgaat gtgcctgagc caccatcggt tcgtaatcga tcgcagggtc gcgcgggtaa
    10381 cagtagagcc caccggggaa gcgccagtcc gccagcgagt agtagaaccc catccgcatg
    10441 cccatggagt gacacgcatc cacgtattcc gcgagcagat cgcgctttgc ggcggtcctc
    10501 ggggcgctga agtccgttgt cttggtgtcc cacaggcaga agccgtcgtg gtgcctcgcc
    10561 gtaagcacca tgtaccgcat gccggcgtcc tgcgcaagag ccacccagtc ccggggcttg
    10621 tagtgcttgg ggttgaactg atcggcgagc ttcgcatact cttccgcggg aatgtgctcc
    10681 cggtacatca cccattcccc gcgcccggga atggcatata ccccccagtg gatgaacatc
    10741 ccgaaccgtg cctgcatgaa ccacgccttc gggtcctcgt gccggtttgt gctcacgtgt
    10801 ttttccttcc ccggaaggtg acgtgtcatc ccgcagctgg ctcgaaactc aagcccgctg
    10861 ccggcctgcc gtcgggatcg gcgagacgca gtgcaaatcc ccacccgccg cccctgttgt
    10921 agcatttgac aacgatctca gatcttcctg ccggcagcgt cgtccacgcc acgttgtcgt
    10981 cgagatggat gcctcgggac cccgtgtaac gccagatttc ctttccgttg acccgtacca
    11041 gcgtatcctt cgcggcgccg agccgcacct gcaccgtccg ctcgaccgcg ctggtgaccg
    11101 tgcacaccgc gtacccgatc gtgttttctg cctttttcac tggcccgatg agatcgagga
    11161 acgcgatgac cccgccacgc gtcccgatcg tttcccagct cgcctgctcg ccatccttgc
    11221 cggcatacgt cgccgtgagg tcaatgccgg catcgggagc gaggttcgta tcgagatcgc
    11281 cactttcgcc cgcgtcgaac gggaacgcga tgcgccacgc cggggggatt ccctgtggct
    11341 cgggttcccg cgcctgaaaa ccgatggggc ggatgcgaat cgctgccatc tcagggcgtt
    11401 ccggaggcac ggtgagggag caggtgcgcc gcacttcatc gttgatctct cgcaccgcgc
    11461 gtgtcatctc cggatcaagg ttgaacccgc cgaagatgtc gttgggattc gtctgatgta
    11521 gaaccgcgaa gcccataaga ccggctgcgc cttcgcggac gaaatcctcc gcctgttctc
    11581 tgatttcgaa tgccgtcaac gggccgcgct tgttccagtc agccccccag aacgcctgat
    11641 acagccccat gaacgggatt cccggaacct gtctccgggc gtacacaagg atcgcctgcg
    11701 tgtccatgga cgtcatttgt cggtcgtagc cccatttcag cacgggatac cagtacggca
    11761 cgatgatatc gcacgccccg gggccgaagt tgaacatcgt gtccgggccg ctgtacccgc
    11821 cgcagacggg gtgatgtcca tccacctcgt gcacaacgct cgtcagaccg cgcattgccg
    11881 aaagcgcgta ccccggcgtg tcgtccagcg cccagtagcc ccagaggttg ggcgagtcct
    11941 tcaccttggc gatctcaagg gcgaacggct ccggccagaa catgatgatg agcgcccgat
    12001 gcggtttcgc ttctgtgccg tccgcgccgc gttcgcaccc gaccagcgtc gtgtcggtcg
    12061 aatcccggta acggatgcgt tcgtcctcga tgacgacgac tcccggcccc ggcgcgggtt
    12121 ttgcccccga ctcgaatggg ataatcgcct cccgcgcgtc gatcgcgcgg gaaaggcgcg
    12181 ggccgccgtg ggtcggaccg gtgatgctgt gcatcgcctt caggccgttg tcgagggcat
    12241 agtgtcccat cggggtatca gggtcgagca tgtccagatg ggcatacgcc gccatgttga
    12301 acccggcgtc gtgcgcaatc tgaagatctt ccagcgaacg cacgttgaac gcgccgagaa
    12361 ccggcacgcc gttgatccag tatgcttcgt ggagccgcat gcgtgtcccc gttctgttgt
    12421 ccgccatcct tgcctcccgt gcggggcgcg cccagccccg cgtcgcagta accaccatgc
    12481 ccagagcggc cgacgccccc agcgcgatga attcgcgccg tgttaggccg ccgccgtcgt
    12541 tttccgttgt cgagtctcgt tggtcttgag gaagagatct cattccgtgc gcctcatggc
    12601 aaaggccgcg cctgccctcg ttctccgtgt gtacgatggg aacttcgcac gccgcgccca
    12661 acccccgcgt gttcctgagc ccgcccgccg ggcagatgac ctcacacgta gcccaaccgc
    12721 gtggaggggc acgctccgtc gtgcccgttt tctgccgcac acgaaatctc tccccacata
    12781 ccagctgagg caaaacgttg tcttcgtgtc aaggggatag gccggtactt ctctcggtat
    12841 gagaagagga ttgttgtgga aggtgttatt tttaatgaga aataggagcg cagaaataag
    12901 ttcgtggggg gtggtggttt tctccattcc tccccctgcg acaacgtgcg tggaggtgcc
    12961 agccgacttt gaggcgtggc actgcactgg tcctgtggga cacataggac ctataccaat
    13021 gtgttcctgt tcggtagccg ctcacacccc cggctcccgt gccgcctcca ccagcgcgat
    13081 gatgttctcc gccggcacgt cctcctgaat ctcctgcgcg ggggcaagta tgtagccccc
    13141 gttcgccgcc atgagcttcc gcacgcgccg cacctccgcc ttcacctcct ccggcgttcc
    13201 gaaggggagc acctgctgcg tgctgatgcc gccccagaac gcgagcttgt caccgtagtc
    13261 ccgcttcagc gccgccaggt ccatgcactc cggctgcacc gggttcagca cgtccagccc
    13321 catctccaca agcgacggaa tgatgcgctc cacgcacccg cacgagtgca cccacacgtc
    13381 cttcccgtag ctgtggacga ggtcatactc cttctgctcg cccgcccgga tcatatcctg
    13441 ccacgtgtcg ggcgacatga gcaggtccac ctgcgagccc cagtcgctgc ccagcaacac
    13501 tccgtctatc tccggcgcca tcaggaaatt ctccagcatc accatgttct tctcgatgat
    13561 gcggttcagc agtttcctcg cgaattccgg gtagcccgcc atgtcggcga ggaaattctc
    13621 gatgccccgc gtgaagtagg ctttctcgaa gtgcgagccg tagatcatcg cgaggatgta
    13681 cttgtcacag ttgtcccgca gcatccggat tgattcgaag aactgcgggt agctgccgat
    13741 tccgatcact gttgccggtg ttgacggtgg atctgccgcc cgccattccg ggccgagttc
    13801 gtgccagttc caccatggca cgccgatccg cacgacgtcg ttgtccacgt attcctgtgc
    13861 cgtctgccgt ccgatggcct cctgcaggcg cttttccccg ccgccggtga agccgatgca
    13921 gtagggcacg cgttccgttt ccttgtgcgc gatggccgct ttgacgattt ctcgccgggt
    13981 catggagtgc agtcctcagg attgggggtg cggggtaaag ctcatcgtgc gagggccctt
    14041 gccaccactt cctgctggat atccggggaa agggtccgga aataggcgct gtacccggag
    14101 acgcgcacga cgaggtccgg atgccgttcg ggatgttcac gggcgtccac cagcgcggaa
    14161 ggatccacaa cgttgatctg aaggacggaa ccgccatagt ccacgaaaca gcgcacgaca
    14221 ctctccagca cggatggatc ggcctgttcg ggcgacaaag gcatcttcac atcgaggacg
    14281 gcaaccaccg ggtagtcggt catgtccacc ggctcaagcg ctctcaaaac ctgcgtgacg
    14341 ttacattcgc ggccgagtgc cagctgactc gggctcattc cgggcgagag atgctcaccc
    14401 gcgtgccgcc catccggcgt cgccccgcca cgacggccga aatcagcgaa cgaccggaaa
    14461 gagaagaggc tggcctcgta cgtgccgcca cgggtgttgg gatagccgct cgctgcccgt
    14521 gcgacgtcgg caaaaacacg cgcggagaac tcgcgcatcc ccggatcgcc gtgtccgaat
    14581 ttcgggatcc ggttgatgat gtactggcgg aacgcttcct cgccctcgaa atctgccgcc
    14641 aggatgtcgc gaaagcgggc gagagatagt ctcttctgtt cgaacacgac cattttcact
    14701 gcgtacaggg agtcgatcag tgtgctaatt ccggcgagag agacgcttcc aaagctgtac
    14761 cgtgcgcctc ccgccatcat gtcgcgggcg cgttcgatgc aatcgctgat agtggccgag
    14821 tggagagggc acgggttgta tcgcgtgccc tccgcctctg tgcggttacg cgcggccaca
    14881 tgagccccga tgactgcccg cagattccgc agaaatgcgg cgtagaactc ctcagacgtc
    14941 cgtgctgcgt ccaacggcac gatcacgaac ccgtcacgcc tggcaagttc gtcccagcgt
    15001 tcggggaaaa agcccatgag aaggacaccc ggcaggctca tgtacatcgt cgcacggctg
    15061 ttgatttccg tgttctccag cacgttctcc tggcagcccc cgccgacgta aagccggcaa
    15121 tcccggaccg ccttgcccat cctggcgttt gcaggaatga tgacgtcatc gttgaagatc
    15181 gagagcacgt tactgccggt gctggagaac tcggccaaca accggaagaa ctcctcctga
    15241 tgcccggagg agacacgcgc gttcagcttg gggtcgacaa gagcgtgctc gcggtacacg
    15301 tcgagtatca tgcgggttat ctcgttgaac acggggacgc cgttcacgtc acacccgcca
    15361 atgaccacag tagtgtttgt gccgacgtgg ttccgtgctt cgcgcatccc gaacgccgca
    15421 tcgcaccgcg ccaagaaaaa cgcgaggagg tccttggcct catcccgcgt caggcggctc
    15481 tcggcgatgt cgttctcgta gaacggaata aggatccggt ccagatgccc gtacacgctg
    15541 atgccgttac cttccagcgc ctgagttgtc tcccgcatga acagaatcgt cgccagtgct
    15601 tcgaaaaacg tctccggcgg gaacgccgga acacgttctg cggtagcagc gatccgggcc
    15661 agtcgcgcct tcactgcagg atcggtttcg actaaaagca tcccgcgcgc ttcgtccgca
    15721 aaacgggtcg cgagatgcat ctgcgccgat aatccggcga tcatcgcctc aaggaacgct
    15781 tgctcttctg tgctgcaccc gcgttcaagg cgggccctcg cctccgcgac caacccagtc
    15841 agtcccgcac gaaacacatt gtcgtacccg aggctgtgat ggttgtcgtc cagaaccgcc
    15901 catccgtgcg agagcccgca ctcactgtac ggccgccaga acgcgctccc gacggcggcg
    15961 agttccctcc cgaaaggttg ctccttcagc caggcaccaa ttcctcctgc gccaaggtcc
    16021 gtgcgctgtt tgccaacacc gatttcatag aagaagggga aatgccgaaa caccttcacc
    16081 gggcattccc gggcgatggt ctcgtaaagg agggtcttca ggcggtatgg tgtccactca
    16141 ggattggcag cgctctccgc gtctagccgc gaccgcatga ccgtgacgtc gttcggcagc
    16201 gtggaataac acgcttcgaa ctcgcgttcg aacgcagttc gttcttctct gaatcccatc
    16261 gaggaaccag ttcccttttg tggaggtgtg cgcccgaaca gaaaagaccg tgttatagcc
    16321 tccggaaggc cattccccgg cgttctgtca acgctgttcg ccgatcagag ccttaccacc
    16381 ccagccccgc cgcctcccat gcctttttca ccgatgccgc ggttacccgc gcggcttctt
    16441 ccgcaggcat ctgagccagt gctttgttga acggctcggg agtgaccggc cccgtgtatc
    16501 ctatctcgtt cagcgcccgg agaaaatcgg ggaggtctat cacgcccgtc tccatcggca
    16561 tggcccgctg atcgtctatg tgcttgtcca gcgccacacc cttcggcgcg tcgttaatgt
    16621 gcacgtacac tacttgctcc ggccggagtt tcctgatctg tgcaagattg ccgccgctgg
    16681 tgaaccagtg ccatgaatcg agcagcactc ccatgttgcc cgttcccacg gcgtcacaca
    16741 gctcgatcat ctgcggcaga gtgtggatga attcgtgttt gaagttcgca cggatcgtct
    16801 tcgggccgag gaattcgatt ccgaatacgc agccgtggtc cttgaggatc aaccctatct
    16861 cccggaatcg cgaccgcata cgctcgaagt gttccgcgaa ggtgaactcg ttcgatcccg
    16921 acatcatcca gctcatgaac gagttgcacc cgaactgccg caggaacgca gcccatttcg
    16981 gaagctcctt gagcccctct cggaacttct cgtcggaacc gccgaagtcc accggaagac
    17041 cccatcctcc gatgcgcagt ccgttctctt tgagcagcgc cttcaatttc tcagcaccgt
    17101 accgttccgg ttcgtggata tcgatatcaa tgcccttgaa accgttcgtt ctggcaagcg
    17161 tggcggcttc ttcaatgttg gcgcgaatgc ctaccgcgcc ggggctcagg ttcggaaaca
    17221 tggttcctcc gttcgggtgt cataccagtg aaaagatcag gaccaaaaac agccgctaga
    17281 gcgcggcacc acagtgtttg cagaatcgcg cgtccaaatc gtgccctgtt ttgccgcact
    17341 ggggacagct tttttctgcg tccgcgcgtt tttttgcctg cgacatttcc accgtcacga
    17401 tgccggtggg tacggcaatg atcgagtatc ccagaatcat caccagcgat gcaagcgtct
    17461 tgcccagtgg cgtgtgcggg gatacatccc cgtaccccac cgtcgtcagc gtgacaattg
    17521 cccagtagat gcttgtcggg atgtccgtga agccattctc ctcgccctcg atcatgtaca
    17581 tgaccgaacc gatgacgacg acgagcatcg agacggtgga aagaaacacg gtgattttgc
    17641 gcccgctcgc aaggagggcg gtggaaagca cgttcgcttc gcgcaggaac ttcgaaagct
    17701 tgaacacccg gaacgcgcgc agaagcctca gcgaacgcac tgacaggaaa tactggctgc
    17761 cgggaagcgc caggctgaga tacgtgggca ggatagagag gatatcaacg agcccgaaga
    17821 aactgaacac atagcgcatc ggcctcttcg ccgtcgcgat ccgcagggcg aattccaccg
    17881 agaaaagaag cgtgaatgcc cattccaggc cgaaaagaac tgacccgaat cggaaccgga
    17941 tgctgcgaac gctctccacg aacacagtca ggacgctcag gacgatcgcg accatcaaca
    18001 cgacatcgaa cagccggccc gctggcgtat cagattcgaa gattatgcga taccatttgt
    18061 cgcgccacgg agctcttctt ttgggctcga tccgtgggtt cgactcaccg tgctgcatct
    18121 cgggtcaagc ctttttgtgc ggctgtgtcg tttccacgcg gagccttgga gcttcgtagt
    18181 cctcaggcac atgctccgct ggtattgcca cctggtttcc gtctctcacc gcgcgatagt
    18241 gaggcgacat gatctccact ccccgctcgt tgaacacgtc ctggatgttc tggtggagct
    18301 cggtgtacgt tgctgccatg gtgtttggca gggcagtaaa cgcgttcaac tcgtactcta
    18361 cgtagaaatc ctccaaagag gtctggtgta cgaagggtgg aggatttttc agcaggctcg
    18421 acgacctgtc cgccgcctcc agaagaagct cgtgaacgac tctccacggc acgtcgtagc
    18481 cgattgtcac tgaggtgtgt actatcagtg tgttccccga cgaactgaaa ttcgtgatgt
    18541 ggctccccag aaccatcgaa ttcggaatcg taatgtcgac gtttttcact gtccgaaccc
    18601 gcgtaacgag cagcgtcttc tccaccaccg tgcctgtcgt gtctgatatt ttcaccatgt
    18661 cccctacgcg gaacgccctt gtatacgtga ggaccacacc ggagacaatg ttggaaacag
    18721 ctccggaaga accgagggaa aacaacacgc cgacgaagat ggatatgccc tgaaaagccg
    18781 gtgaaccgga ccctggtaag taggggaaca ggacgacggc gcagaaaacg atgataagga
    18841 aacgaacgat cttgtacgtc ggtgctgccc actcgggaaa gaaccctggc agggtcaccg
    18901 tgcctttgcc gatctctgaa gccgtccatc tcacggcttt cactatggcg tacgttaccg
    18961 caaggatgac gatgatggcg aaaagattgg gtaggtaccc aaccacggta tccccaacct
    19021 ggcggaccgg agtgaccagc atctcgacga tacttcccac ccaggtggag gtaactgcga
    19081 acaagcccaa cacgaaggcg gcgtacccgt aaacagcaag taccaacagc atgacgcgga
    19141 gaatctggat accggtgtgc ccgagatgaa ccatcttcac gtaaagggtt gtttcttcgg
    19201 ggcttgcctc caattcggcc gcccaatccc gcgaacgggc ggcgaccgct gcgccaaagc
    19261 ggttgatcgc ccgcacgacc acggcgagca gaaccgtggc caacacggca tacatgacac
    19321 cgcgcacgac ttcataggag gcctgggctt ctcctgccgg gaacagggag gtgaaatagc
    19381 ggccgacgac gtagaacgag acaagcgcga aggagacgcg aagggccttc gaactgaaag
    19441 tgagaaaatc cgctaccgtt tcgacttcag cgagtttgtg gcctcgaatc gtcaacggcg
    19501 agcgccaatt ctcgaggcgt cggtagacac ctgggaagga tccgttgatc cggaaaaaca
    19561 cgacaaggcc aagcagggag atgagagacc cctgcaggta gacgagtccc tcgtcttgaa
    19621 ggtcgaacaa atcgagagcc atgctggcag cgaagtagaa cagcgcaagg gaggcgcaga
    19681 cgttgaccag ccacaacgcc gtttctacgg ttgctcgcac cgccctgtgc catagtggtg
    19741 tcgcgcggcc ctcgccgggg tgctccgcca agagcttcga gacgagtgtc tgtgccagaa
    19801 aacggcgtac ccgcccgcca aacgagagga cgagaatact taacagcaaa aggcagacga
    19861 cggcgcagat ggaaatcgcc agggcgaccg cgctgctcga ttggaggttg cctcccgaga
    19921 acgccgcctg cagaccgaca gcaaacgcca acggtttcat ccgcatttcc ttccgtgagt
    19981 aaggcacctg gccgggcgga agcgctgtat ggtacgcaat atacgcaacg ggaggccggc
    20041 cacgcatccg ccgagccgag cggagtagca gcggtcgccc cttctacggc cggtgccaac
    20101 caccacgggc tggcagtgcc cttgtgttac tccggtgggt gcgcatatca taccggtgtg
    20161 atgcagagta gcggatctgt ttcgcatgcg cagcttcgtg ggagggcccg gacatgaacg
    20221 gtgagcaagt aacgttttcg gtattcacca agccgtggaa gacactgtcg cttggtgaac
    20281 tgggagcgtt ggtacggggg ttcggattcg atgcggtgga gctccccgtt cgacccggct
    20341 atcaggtgca gccggacacc atggaaaccg gcttgaaaga agcagttcgg gcatttgcgg
    20401 atgagggaat agtggtaaaa agcgtcgcgg cgccgactga cggcgtacgc cccggcttca
    20461 ttggggagct tatcgcggcg tgcgccgaga atgatattcc ggtgattcgc gtgttcccgt
    20521 tcatccgccc cgatgaaccc tatctggagg gcgaggcgcg cgttcagaat gaactggaaa
    20581 gccttctgcc ggcgctcgaa caatgtgggg tgacagttgg cgttcagaac cactgtggac
    20641 cgtgtttcac cggcgcgatg catctcttcc acctgataga acgtttcgat ccgcaacata
    20701 tcggagcgat cctcgatgcc gggcacaccg gtcttgccgg agaggaaccc gaacatgcga
    20761 tcgacacggt ctggtcgcat ctctgcatgg tgaatttcaa aaatgcattc cgccgactgc
    20821 gaaccgggcc ggaagcggaa caggcagagt ggcaggtgta ctggaccagt gcccgccacg
    20881 ggttgtccga ctggagacgg tacgccgcgg aactgaaaaa gcggcgatgg cggggcacag
    20941 catgcctcac ggcggaatac tcggatccga acgcggtgga ccgccttatc gcagacgatc
    21001 tgaggtacac gaagtccctt tttgtctaac gccggctatc agtcggaagg gggatgccat
    21061 gggaacagat cgtccgaaca tcgtgttttt tctcgcggat gatcagggcg catgggcact
    21121 cgggtgcgca ggtaaccggg aaatccggac cccgaacctt gatcggcttg ccgcggaagg
    21181 tatccgattc acgaacttct tctgcgcgtc tccggtctgt tcgcctgccc gagccagcat
    21241 cctcaccggt agaatttcct cccagcacgg cattcatgac tggatacgcg cgggaaatgt
    21301 gccgaacgaa gtgcccggag tgacgcacgg aaggcctgtt gaataccttc gcggcatgat
    21361 cgggtacacg gatgtccttg cagaaaacgg gtatacgtgc ggcctgagcg ggaaatggca
    21421 cctcggggat tcccttcgcc cgcagaaggg gttctccttc tggaatgtcc acgcgcgcgg
    21481 cgggagcgga tattatgatg ctccgatgat tcgggacggt atcgcgtaca cagaaccgcg
    21541 atatgtgacc gacgtcattg cggagaacgc cgttgcgttt ctgcgggagc agcaggcaac
    21601 ggggacgccg ttctatctca gcgttcatta caccgctccg cacagcccgt ggactccgga
    21661 tcagcaccca cggcggacgt acgatgagta ttaccaacgc taccctttca attcgacgcc
    21721 cttcgaaccg atgcacccct ggcagatttc cagcgcgcct actggcttcg acgaactgag
    21781 ccgccgccgc gaactgagtg ggtattacac cgccatcacc gagatggacc gcactatcgg
    21841 cgatgtgatc gcagaactgg agaggcaggg catcaggcag aacacactcg tcgtgttcac
    21901 gagcgacaac gggatgaaca tggggcacca cggtctctat ggcaagggaa acgcgacctt
    21961 tccggcgaac atgtttgaca cgtcggtgaa ggtcccgatg attgtctccc gtcctggttc
    22021 tgttccttcc ggtgttgtgt gcgacgaaat gctgagccac tacgaccttc tgcccacgtt
    22081 tctcgactac gtgaacgcgg aaaaccccgt tgcgggcgaa actcccggtg tgagttttgt
    22141 cccgatcctt cgtggggatg agttcgctcg tcgcgacgcg gtggtggttt tcgatgaata
    22201 cgggccgacg cgcatgataa ggacgaagga gtggaaatac gtccatcgtt acccgtacgg
    22261 tcctcacgaa ttgtatgacc tcgtttccga tccggaggaa cgcgacaacg tagtggaaga
    22321 tgcacgttgt cagaatgttc tgcgtgatct gcgggcccgc ctgtccgagt ggttcgttca
    22381 atgggccgat ccggcgcgag acgggtcgcg agaaccggtc cgcggcgttg gccagcacga
    22441 tcttgcgggc ccggccggcg caggagtggt cgccttcaat caggatttcc gctttctcag
    22501 cacatctcac gggcagtagc gttcattcgg agcgcgcgac gtcaggcatt ccgcgtgcgg
    22561 ggtggttgct gctgggacga aagggaagga acccatgttc aacgcgttcc cgagagacga
    22621 aacaatcacg cggcgcgagc gtttctgccg cctctacgac ttcgagcctg ttgattcgca
    22681 cgtgcgatgg gaagcagtgg cgttttgggg tgacaccgcg cgggattggg ttgcggaagg
    22741 cgggctgccg gaagacgcgg accccatgga ttactacggc atggacccga ggaacggcat
    22801 cagtggcaac ctcgggttca cttccatggg gctctcgggt ccgaggccgg aaactcatgt
    22861 gatcgccgaa gaaggccgta ccagggtgta ccggaacgac ctcggccagg tatggcgtga
    22921 ccgggtggat ggagtcagca tgccgcagtg gatacgcttc cccgtggaat cgagggacga
    22981 ttgggagcgc atcatcaagc ctcgcctgaa acccgaagat cacgactttg gcaatctgga
    23041 tgaggaagcg gtccggtcgc gaacttccca agacgcgtgg gggttgtgga tcgttggcct
    23101 gtacgcattc tggaggaatt tctgggggga ggagaaactt gcgttcgcgt tctatgacca
    23161 accggacgtg ttgcacgata tggcgaaatg ctggctcacg atgcattgcg aatgcacgcc
    23221 gcgtgttctc gataccgccc ggatagacta tgtcatgttc cacgaggaca tggcgttccg
    23281 caacggcccc ctcatcggac cgcagctttt tgattcattc atggcgccct attaccggga
    23341 gctgttcgtt catcttagga atcgcgggca acaccggttt ctcctggact ccgacggcaa
    23401 caacggaccg attctggagc ggtttgccga cctcggaatg aacggtctct acccctttga
    23461 agccgccgcc ggttgcgacg cgatcgcgtt ccgcaaggaa cacccccggt tttttgtctg
    23521 gggcgcgatc gacaaacgtg tgcttttggg aaacaaagac gatgtggagc gggaagtgat
    23581 ggccaaagtt cccgcgctgt gggcgggggg cggcttcatc ccttccatag accactctgt
    23641 tccgccgtgc agtcaggaaa acttcgagca cttcatcaac ctcgtccgga gcatctgcag
    23701 atgaatcgca gcaaccgatc tggaagggaa tccgcaatgg cgatccggaa gctcgaacca
    23761 gtacgcgtga acccggtgct ccgccatccg gatgagcaaa gggtgagcct cgacggtcgg
    23821 tggctgttcc gcctcgatcc cgaggatatc ggcgtccgcg aacgttggtt ccgagatccc
    23881 gcggttctcg acgcttccgt gcaggtgccc gggtgctggc aaggccaggg ccacggcggc
    23941 gatggcaacg acagggtgtg ggatttcgga cttgaggaac ggaccttccg cgccacctac
    24001 aagggcacgg ggtggtatgg acgcgcgttc accccaccgg acgagtggca gggaagacgc
    24061 gtgcaggttc ttttcggtgg cgtccatccg agtgcggagg tctggttgaa cggcgagcgc
    24121 ctcggcgaga acggcatgcc cttcgttccc ttcgggttcg acatcacgag caacctccgt
    24181 ttctgttccg agaacctgct ggtagtgagg gtgcacgagg cggcccggat ctacggcctg
    24241 gcgtacagtt ggcagggcaa ctggtcgggt ctgtaccggg gagtggagct gaccgcgaca
    24301 ggctccaccc gcatcgcgcg gtgcagcgct tatccagaag tcgccgccga agcgatgcga
    24361 gttgccatcg aattggacgc ggtggcgccg agaggggcga agatccgtgc ttccgtgatc
    24421 gccatcggcg gcaacagcgc gccaataacc gtggaagcgc cggcaaaggg caagaacatc
    24481 gagttccgcg taccggtgcc gtcgccccgg ctctggagcc ccgaaacgcc cggtctctac
    24541 cgcgtggaca ttgcccttgt tgttgggagc gtcgtcatcg atgcactgag cgaacgaacc
    24601 ggattcgtgc agctttccac ccgcaagaac cagttcctga tcaacggaga accctactac
    24661 atgcgcgg
//
LOCUS       DAOSOO010000023        24329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2078_length_24329_cov_5.924569, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000023 DAOSOO010000000
VERSION     DAOSOO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24329
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2078_length_24329_cov_5.924569"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1350)
                     /locus_tag="PLT86_03070"
     CDS             complement(175..1350)
                     /locus_tag="PLT86_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35628.1"
                     /translation="MTKSKLRRIGVGMLGYGFMGKVHTECYRMLRMWCETLPVEVHFV
                     GVATKHVETAEFAVEQAGYGFGTTDWRQVVEHPDVDVVNICTPNALHFEQAMGAIQSG
                     KHVYCDKPLATNPDHARTMANAAQAAGIVHQVTQNYRFVPATLRAKQLVDEGLLGRPY
                     SFRAAYLHSGSSDPERPAGWKMQDGGTLHDLGAHVVDLVRHLWGDFAEVLAHRQVFVP
                     SRPIRAGSSERVAISGDDAAWMLTKLANGAIGTIETNKVATGAEDELRIELHGEKGAI
                     RFNLMEPNWLDVYDARAEDGPYGGNRGFLRIATCSRQKEPSVIPPPRGAAGWMQFHLA
                     SLHSFVGNVLRGSAGHPDFFDGWAVHEVLAAAEESSRTGTWVNVRSVNCRAEAEEQR"
     gene            1510..2403
                     /locus_tag="PLT86_03075"
     CDS             1510..2403
                     /locus_tag="PLT86_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT35629.1"
                     /translation="MREVRWERMFPDELEKAFSECPVVYFAYGLCEPHGPQNAIGLDA
                     LKAHAICCRAAERHGGIVAPCDFWHIHELGGYAVWSRQAIGEVERSWLTCMPPWVHLK
                     NVCYHVRQADVLGFHAAILFTGHYGPNWEDLKTLVRLIQPYVGTRLYSLPECEANVPG
                     FDGDGASGDHAGKVETSLLWALMPECTDVSRLPDKETGPAPWAMGRNAYEASRRIGER
                     MVEDEVTWLGRKAGELLEEYEKSRPSHTLRTFEDVERLWEGVVRPHVPEFRSMQLCWK
                     EHQEVPGDSVWYSNWKVPERA"
     gene            2612..5209
                     /gene="alaS"
                     /locus_tag="PLT86_03080"
     CDS             2612..5209
                     /gene="alaS"
                     /locus_tag="PLT86_03080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT35630.1"
                     /translation="MTSSELRRGFLDYFAKRGHRIVPSSPLIPHDDPTLLFANAGMNQ
                     FKDIFLGQRVPEFRRAASSQKCIRAGGKHNDLEDVGKDGTHHTFFEMLGNWSFGDYYK
                     AEAIEWAWEYLTSELQLPKDRLRATVHHSDDEAEELWAKIAGIPRSHISRHGDKDNFW
                     EMAEVGPCGPCTEIHLDMGADRGCGRPDCGPNCKYCDEVHDARFMELWNLVFMQYYRD
                     ENGVLTPLPETHVDTGAGFERLCSVIQGKSSNYDTDLFMPIIRATEEKTGRGYVSGVE
                     GTPFRIIADHMRALTLAITDGGFPSNVGRGYVLRRILRRASRAGRQLGLREPFLYELT
                     NSVVDTMGSAYPELEERRTHVATVIKREEEQFGRTLNRGIDQFETFAAELAATGSKVL
                     DGEKVFQLYDTYGVPVDLTALMAEDMKLTIDLDGFNRCMEEQRERSRAQAKFDRAVTN
                     FGEGLVSEFVGYDTLSTEAQITGLAEGAIVLDQTPFYAESGGQVADVGELFNAEFSFE
                     VTGVQKQGTAIVHFGKVTRGAPSVGMAVTAQVNSDLRGRTMRNHSATHLLQAALRAAL
                     GPQVHQAGSFVSPDRLRFDFTCGEAVPPEVLRQVEATVNARIRENLAVNISHSTLDEA
                     KASGAIALFGEKYGETVRVVRMGNFTMELCGGTHVDATGQIGYFRILSESGVAAGTRR
                     IEAVTGVTAEELAREDRDRLARISAILKVGVDDVEPRITALLDRLKEIERELEKAQTA
                     MAGADVDAWIREAGEIGDARLLIREVTLADPKAFRALGDTVREKLGKGVALLIGHDAG
                     KLSLMLLVTDNLTSRIQAGKLMNELAAIVGARGGGRAHMAQAGGGSPERLADLLAKAP
                     GVVARELGI"
     gene            5329..6249
                     /locus_tag="PLT86_03085"
     CDS             5329..6249
                     /locus_tag="PLT86_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT35631.1"
                     /translation="MSGIAFVLLLLGSAQADTILSRGSSNEWCWRCHGMATFSVLRAD
                     GTIRTLSADSTAFLASNHRRLRCVTCHSWEDVQVFPHGKNAHMAQTCTDCHVAESHPL
                     NRTDPLQAMHFREITDEFRKSVHYQTEDHAFSCFSCHDPHRFRKSENPSLQTIGTQNG
                     VCLGCHGDAARFATLSERRIDLSATHAWLPRRELHWEHVRCLDCHTSYAPPNLSHNIL
                     PADSAVHACEQCHRQNSILLDKLYRYRHVEERSRNGFVNAALVNDSYVIGSTRNRYLD
                     LLGAVLVGCACVGVAGHGFGRWHSARKKRK"
     gene            6258..6926
                     /locus_tag="PLT86_03090"
     CDS             6258..6926
                     /locus_tag="PLT86_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOT35632.1"
                     /translation="MDTVHREYLHPLWVRIWHWLNAALFLALAVSGFRLHFSGSAVQL
                     LPFRTAILVHNASGALFTIALILYAILLGITGEWRQYLGLRPRMLALVGQNLRFYLID
                     VFKGAPHPESPTRTQKFNALQQVTYLFVLVVGMVPVAITGVMLSVPETAPKQILGAGG
                     IWPVAVLHTLLAYGLVAFFVGHVYLTTMGPTLWSDIKSMFTGWHERVHGQADIQPQEQ
                     GDTP"
     gene            6923..9190
                     /locus_tag="PLT86_03095"
     CDS             6923..9190
                     /locus_tag="PLT86_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HOT35633.1"
                     /translation="MTDTPQRTSNESFPCPGWWKRVFRNRRLRKWSFGILVAVVCGLF
                     IRYVLVPWGFQKYTGVVNAELSYPNSKAVSEAEFEAIAADLRSEGVLRRALALTSSVT
                     DSITRKTRIRMIAATPSFQRQEEFAHIRYFRDAGIRRYEGPKTCIRCHETIDVRKPDG
                     TIQKVKTIPDVLNTIHYKFQSMDGGFTTYGYDGRQVNTGTRGIPVGKIDRACGVPGSF
                     SWTGWAALIETKPAHAGGEVVLRSEGCGQCHIGGGYHPATENMMPGLVVPTPKVAAEG
                     LDCLICHASAYDMNQRFVIRDEHGLRWNQDRSLRAALSVGRPTNENCLNCHQHNMGGD
                     TYAYNTSAQSLGTQNQRLLHSTAKRGNPFSVHDDVHARAGLVCLDCHRPQGHKIPRGN
                     KGTDLVANDLPGVKVECEMCHTSAPHVRNRRTRAALNGHADYIACETCHIARLLPFNN
                     VLKDWVHPVWNEEEGMYVPKAVYSSGDPNRGLAYLWFNGNGTFLANALGANPNHSPDY
                     NPLMRQMVVIEDPVVLGEIAANTRDIRTRYGLDSASYMAPIANALSQLSPEMLSRRRE
                     MIERNLRVRMNEGKSRIYPFKLFNAMMFEDLNNEGPFGAMILPFDYRTYFETGDAENA
                     VKVAVANPIVKRMYETPFKLYMMDEFMAYFGVGKWTAKYPLDEGNWNVQPRWMRQMGT
                     LMVNHGIQPVGRQCAECHNRNGILDFAALGYTTDRVRALQNLPELSYFQPPLRPSHRQ
                     EGAEVQAEATNASER"
     gene            9174..10634
                     /locus_tag="PLT86_03100"
     CDS             9174..10634
                     /locus_tag="PLT86_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOT35634.1"
                     /translation="MLQNDKIWVGKGANPVYLLPTMANRHGLIAGATGTGKTVTLKVV
                     AEAFSDMGVPVFAADIKGDLAGIAVAGTPNPKVEERLRKLGVEGFQYAGFPVRFWDLF
                     GEAGHPVRATVSDMGALLLSRMLGLNDTQAGVLNITFRVADDNGLLLLDLADLRAMLQ
                     YVGDHASELTTRYGNVSIQSVGAILRALLTLEDQGGAKFFGEPALDISDWMQVDAQGK
                     GYINILHSVKLYMSPALYSTFLLWMLSELFEVLPEAGDLKKPKMVFFFDEAHLLFKDA
                     PKALLEKIQQVVRLIRSKGVGVYFVTQVPSDVPQEVLGQLGNRVQHALRAFTPADQKF
                     VKAAARSFRANPSLDSEKTILELGTGEALVSFLDADGKPSIAERAMILPPQSSFSAID
                     DSTRSRIISASLLGRKYETAVNRKSAFETLQAESQAPREAPRERTTTPKRVPTGTTGR
                     RTRTPLEAAAGSVMTTIGREVGRQIVRGILGSILKR"
     gene            10729..11505
                     /gene="hisF"
                     /locus_tag="PLT86_03105"
     CDS             10729..11505
                     /gene="hisF"
                     /locus_tag="PLT86_03105"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT35635.1"
                     /translation="MLTKRIIPCLDVRAGKVTKGVAFQGNIDVGDPVAMAKHYYEQGA
                     DEIVFYDITASNERRDIMIDVVASVAREIFIPFSVGGGLRTLSDMRRVLLAGAEKVSI
                     DSGAVRNPDTIRQGAEAFGRQCIVLSMQVKRVPQTPTIPSGYEIYIDGARTATGLDAI
                     EWAQRGEELGAGELVVNSIDADGVKTGYDVDLTRRMAEAVGIPVIASGGAGEPSHLYD
                     VFSQGNADAAIVASMIHFGTYSIPEIKEYLHRRGLPIRMV"
     gene            complement(11570..12352)
                     /locus_tag="PLT86_03110"
     CDS             complement(11570..12352)
                     /locus_tag="PLT86_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOT35636.1"
                     /translation="MATKPPVAVVTGAGRGIGRGIALGLAKANFDVAVNFAHNAEAAA
                     ETCEEISRLGRHAMPVRADIADADDRAQLIAETENRLGPVTLLVNNAGIAPEVRADVL
                     ESTEESFDRLVRTNLKGPYFLTQIVATKMIEWQRAGLVREPKIVFITSVSAFAPSVNR
                     GDYCISKAGLAMAAKVFAARLAGDGIGVYEVQPGVIQTDMTEKVRERYDKLILEGNLL
                     PIKRWGQPEDVAHAVVAIAEGRFSYSTGITIVVDGGFHLRVL"
     gene            complement(12383..13294)
                     /locus_tag="PLT86_03115"
     CDS             complement(12383..13294)
                     /locus_tag="PLT86_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOT35637.1"
                     /translation="MAPSFSIPRLEDLAGCYRYCRKITHLYGANFSVGFRCLPKRKRD
                     AVYAVYAFCRFADDIADEYTGHENPEALLDAWEKALERCYQGVTDHPILVALADAAAR
                     FPIPLESFRRLIEGCRMDFVKTRYATFEELLVYCDRVATTISELSLAIFGWTDPRTPR
                     WGTELSIALQMTNILRDVAEDARRGRIYVPQEDLVRFGCREEDLKSREPAPGFLQLME
                     FELERVFRYFDLARPLCGAVSPDSRMAVALMGSVYREVALQISRNTHSVLVGKVQLTT
                     LQKLVLVVNAAARSAFHRLGVSGPPCW"
     gene            complement(13304..>13693)
                     /locus_tag="PLT86_03120"
     CDS             complement(13304..>13693)
                     /locus_tag="PLT86_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35638.1"
                     /translation="NMRPLHVGEAGGFADHLFGFGIRLALQSGVLAAQCVLSGRAFGP
                     AWEEHFGQRFEMGLANRFLYETGGHLAYRWFLSKASCSDFREVGQALYKPNAAKRALA
                     VLARAVWKKQPDCIHGAQCVWCRLQKG"
     assembly_gap    13694..13793
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13794..14164)
                     /locus_tag="PLT86_03125"
     CDS             complement(<13794..14164)
                     /locus_tag="PLT86_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOT35639.1"
                     /translation="MENQEHVVIAGAGPAGLASAITLARAGLSVEVRELRGDVGGHFR
                     GNLQLLTNYPDLEDARTELCSLLGSVPVSLWPQRSAQLFGPSGKCVSGRSSEPFGYLL
                     HRGPDPTALDGALKRAAEEAGV"
     gene            complement(14564..15529)
                     /locus_tag="PLT86_03130"
     CDS             complement(14564..15529)
                     /locus_tag="PLT86_03130"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOT35640.1"
                     /translation="MQDTSGNRRKVVVVGAGAVGATFAYALAQSGLADEIVLIDRNED
                     LVRGQVLDLAHGQPFFPTVSIRKGDASDYASASLIVITAGAAQRPGETRLQLLQKNAA
                     VIKGVMTDIIEQASPAVILVVTNPVDVMTYVAIETSGWDRSRVIGSGTVLDSARLEHL
                     LSAHCGVDVHNVHGFVLGEHGDTEFVAWSLTHIAGMQMDAYCPICKGCADNLAEREAI
                     ARQVRDSAYHIIGYKGATWFAVGLALVRIAGAILRNQRTVLTVSALLQGEFGLNDVCI
                     GVPCILSARGIERVVESVLSECELVALHSSASTLKKAVEQLHAGA"
     gene            complement(15596..16426)
                     /gene="proC"
                     /locus_tag="PLT86_03135"
     CDS             complement(15596..16426)
                     /gene="proC"
                     /locus_tag="PLT86_03135"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT35641.1"
                     /translation="MDTTLRIGIIGGGQMGTALVKGILHAGVADASEITVSDVIASAR
                     ERLHAESGVRTTNDNTEAAKNADVVVIALKPQVAAEALEPVAGKLMPTQTVLSIMAGV
                     PTGKLERWLSGDDEAANPVHVVRAMPNTPALIGEGMAAISRGAHATDADMNRAERILA
                     AVGRVVRVDEKQLDAVTGLSGSGPGYVYTVIEALADGGVKVGLPKPLALELAAQTVLG
                     AARMVLETEEHPAVLRDRVTSPGGTTIAGLHAMEAAGIRNALMQAVEAAARRSRELGE
                     "
     gene            16616..17302
                     /locus_tag="PLT86_03140"
     CDS             16616..17302
                     /locus_tag="PLT86_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT35642.1"
                     /translation="MPGQHVAIIEDEDDILELISYNLRKEGYRVTTATSGEAGLLAVR
                     NTLPDLVLLDLMLPGVDGFEVCRSLKKSAETSHIPIIMLTAKGEETDVVAGLELGADD
                     YITKPFSPRVLLARVRAILRRKPKTEDEGSSPIVVHDVAIHPGRHEVTVGGVHVALTH
                     TEFNLLHLLASRPGWVFTRYQIVEAIRGDNSPVTDRSVDVHVVGLRKKLGQAGRYIET
                     VRGMGYRFKG"
     gene            17307..19079
                     /locus_tag="PLT86_03145"
     CDS             17307..19079
                     /locus_tag="PLT86_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35643.1"
                     /translation="MRRVTFAWHLVPVVVLIVWGASLLSAMYAARAIRTFHAGQTGAE
                     LAAQAKLVRQVITPFLVQANPASVDSICKVFGVASSSRITVVSKSGTVLGETGPIAAR
                     LDNHADRPEIIAAFSGRTGSAVRFSHTTRRTMMYTAIPVFQGGEPVAAVRVAKPIDSI
                     EFLVVHLQWRFFLAGAVVALVATVAVLIAIRRATDPLETMRRVAHAYSRGDFRESLPT
                     SRVSEVDDLAQSLNAMARQLDERIRSLVRQRNEQEAVLSSMVEGVIAVDSEERVINLN
                     LAAARLVGADIARSHGRLIQEVVRNTELQQIIRRALDSHEPVEGEIAIRSEPRVFLQA
                     HGTVLRDGTGECIGALVVLNDITRLRTLENMRKEFVANVSHELKTPITSIKASVETLL
                     DGAMENREMARRFLDIVVRHADRLNAIVDDLLSLSRIEQEAENRQIELDALDVEPVLQ
                     AAVQSCLVTADERGINIQVSCANDLKARMNAPLIEQAVVNLIDNATKYSDPGGVVHVV
                     AEQKGTEVRIDVRDTGCGIAPEHLQRVFERFYRVDKARSRNAGGTGLGLSIVKHIVQA
                     HGGRVTVESALGKGSTFSLFLPAA"
     gene            complement(19104..21257)
                     /locus_tag="PLT86_03150"
     CDS             complement(19104..21257)
                     /locus_tag="PLT86_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35644.1"
                     /translation="MTFLTGHFSCVVFRSISGFVLLVLLLASSRSLAGELIPAAGAPA
                     NALRIPPGRLAVPPRIDGIWTPAEWSGAIRVDDFHCHDWNRAPTESTVVWMASDDRGL
                     YFFARCLDRNPDKLRMEERKRLQLAYKGNAVPSLSDDFISFEIDAENLRRSNGAYAFT
                     VTPRGTQADFIPDGAATKIEWRGDWTAAARIDSAGWTVEVSVPYSMLGLPSGKNTVGV
                     SIRRWVPRLQEESRWPNMGFAWDRTRIAHWENLDVGSTGRAFPLVMPYGVAQARDGRF
                     DGYVGLDVKKTFPSGQTFIGCVYPDFRNVENDVLGLDFSYSEKQRADSRPFFVEGYRF
                     LPDAWMMYGQRIGEVYGGGKYFGQIRNHRIGVLTVYDREEVLHSAARWYWQPVPRLEF
                     ENNVVWRHAPPNTPVRSGVPMVEDNLLFVSLVKKSRMIGSLTEYMRLQTGFAQSDGDS
                     ADGFNLEGDYQIIPTASGPSLVVKARHLGNGMTPVDGLLDPIDFDQRDASIELGYERT
                     ADRQWFREWNVSGFVHRSVRSNGNLYLQRYTVESWTKARGGTELSTEFRVEERPPYRD
                     WTFLHEFGWKQDQTYQKGGISARWGWLQGADYLLVTLDQGIHSWDRVTASARLQFRRR
                     DFPAGHALRPTGGVEDRYQVVTTVQYDLTAERSISGRLTYNDDGSGNAWKSRFNGYCS
                     YRQLVRNGFDLFLIVGDPSAETWTRRVAIKAMVVL"
     gene            21602..22168
                     /locus_tag="PLT86_03155"
     CDS             21602..22168
                     /locus_tag="PLT86_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35645.1"
                     /translation="MTFSRNPAFIAAIILLALQGCERTRVTPPVEMRNLRVTPTKIGL
                     DITFELTDSRGQPTIMMFGNGVVRIDDSPDSVQKEVFPGHPAGSSVLDSTWLLYNARL
                     FVTREHFVIQRGGDSTRGQDKYLCALGVYPYSHFLRKPRRPTGNVRVALVGMDGRRVL
                     LGHAPVIWPSEAFSATSGTGDQSRFGNR"
     gene            complement(22199..23359)
                     /locus_tag="PLT86_03160"
     CDS             complement(22199..23359)
                     /locus_tag="PLT86_03160"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOT35646.1"
                     /translation="MSPEEISRLQVRRIVGLMSGTSVDGIDAALVEISGHGTALRLER
                     VRAAETFSFPHEVQEQIHALFDGHVRELCQMNFALGELFAEAALNVIRSAGFAPADVH
                     LIGSHGQTVYHVPRGEGRTASSLQIGEPAVIAERTGITTIGDFRPRDIATGGEGAPLV
                     PYADWVLCRRNEEAIALQNIGGIANLTVVTPRLEDVRAFDTGPGNMVIDGAVTLMTRG
                     SRTYDEDGRIAASARPDEGLVRDLLDDDYFRQPPPKSTGREQWGRQFVRGVVERRGVR
                     PDAVLVSTLTEFVARSIADAYARFVLPHGPLSSVYLSGGGARNPVLRRRITELLAPVP
                     VGDSSVLGLPVDGKEAIAFAVLANETVCGEPSNVPGATGARGRRVLGKIVPA"
     gene            complement(23372..>24329)
                     /locus_tag="PLT86_03165"
     CDS             complement(23372..>24329)
                     /locus_tag="PLT86_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35647.1"
                     /translation="SVLVRDGFAERVQTMVSLADRERSATLAEELVNEAVESLTALGT
                     AATSLRFAEGAVRGLPLTPREVDASAEAFFVRAKPLGAVAVLVADSYARGCADRASDL
                     DVRLLAHEIPDPRSRLRAFAPLADRGIVRQYPGDDVIHADQMVIAGRRVDLRFHTIRS
                     LEVALQGPFIPGGAIEALELLQTNQIILDPQGIATGLVRRSDLLAQRAREIATVSLSA
                     LREWDEATVKSAGSAAEAMVRTLGPGLEYLIRAWAGLNGRVHAFPRWVEAAVLPLPIA
                     PRDGRRTLQRLVTTPWTAQDIPARLSEWNALFLALSELDHPL"
BASE COUNT         5031 a         7444 c         7027 g         4727 t
ORIGIN      
        1 ccagatggtt cggtacgttc attatctgcc attgccgcca cccaccatcg ccagccatag
       61 caatctgacc cgcgccgatc ccgcccagcg gcatggcgac tgctctcaat gccttgccga
      121 gaatcacgcg acgatcgggt ctgccagcca gagcctccgg caatgtgttt ccactcatcg
      181 ttgttcctcc gcttcagccc gacaattcac agatcgcacg ttcacccatg ttccagttcg
      241 actcgattcc tccgccgcgg cgagaacctc gtgcacagcc cagccatcga agaaatcggg
      301 atgccccgca ctccctcgca gaacgtttcc tacgaacgag tgcaggctgg cgagatggaa
      361 ttgcatccac ccggccgcgc cgcgaggcgg cggtatcacc gaaggttcct tctgacggct
      421 gcaggtggcg atgcggagaa agcctctgtt cccgccgtac ggtccatctt cggcacgcgc
      481 gtcgtacacg tcgagccagt tcggctccat gaggttgaac cggattgctc ccttttcccc
      541 gtggagttct atccgcagtt cgtcctccgc acctgtggca accttgtttg tctcgattgt
      601 gccgatcgct ccatttgcga gcttcgtgag catccacgcg gcgtcatctc ccgatatcgc
      661 gacccgctcc gaagagcctg ctcttatcgg acgcgacggc acgaaaacct gccggtgcgc
      721 cagaacctcg gcgaaatctc cccacagatg acgaaccaga tccacaacgt gcgcgcccag
      781 atcgtgcaac gtcccgccat cctgcatttt ccatcctgca ggccgttccg ggtcgctgga
      841 tccgctgtgc aggtacgccg cgcggaagct gtacggtctt ccgagcagtc cttcgtccac
      901 gagttgtttg gctcgcagag tggccggcac gaatcgatag ttctgcgtaa cttggtggac
      961 gatgcccgcc gcctgtgctg cgttcgccat ggtcctcgcg tggtcggggt tcgtggcgag
     1021 gggtttgtcg cagtacacgt gtttgcccga ctgaatcgcg cccatcgctt gctcgaaatg
     1081 cagggcgtta ggcgtgcaaa tgttcaccac gtccacatcc ggatgctcaa caacctgccg
     1141 ccagtctgtc gttccaaacc catacccggc ctgttccacg gcgaactcgg cggtttcgac
     1201 gtgtttcgtt gccacaccga caaagtgcac ttccacgggc agcgtctcgc accacatccg
     1261 aagcattcgg tagcactcgg tgtgaacttt gcccatgaat ccgtacccga gcatgcccac
     1321 cccgatacgg cgaagttttg atttcgtcat atggaacctc ctgtcgaacg attccctgaa
     1381 aggtaggcgt tgtgctgttc ccgctcaaac caagaggccc ctcttgctcc cgcctcagtt
     1441 gtgtggcatt atccggctat caaaggggct ggacctgatt gattcggaaa ctcaaaggga
     1501 ggcatcgcca tgcgcgaggt acggtgggaa cggatgttcc cggacgagct ggaaaaggcg
     1561 ttctctgagt gcccggttgt gtatttcgca tacggacttt gcgaaccgca cggtccccag
     1621 aatgcaatcg gtctggatgc gctcaaggcg cacgccatct gttgccgagc cgcagaacga
     1681 catggcggca tcgtcgcacc gtgcgatttc tggcacatcc acgaactcgg ggggtatgcc
     1741 gtgtggtcgc gccaggccat cggcgaggtg gaacgaagct ggttgacctg catgccgccg
     1801 tgggtgcatc tcaaaaacgt ctgctaccac gtgcgacagg ccgatgtcct gggtttccac
     1861 gcggctatcc tgtttaccgg acattacgga ccgaactggg aggacttaaa aaccctggtt
     1921 agactgattc agccctacgt cgggacccgc ctctattcat tgcccgaatg tgaagccaat
     1981 gttcccggtt ttgatggcga cggagcgagc ggcgaccatg ccgggaaagt ggaaacctcg
     2041 cttctctggg cgcttatgcc ggaatgcaca gacgtgtccc gtctgccgga caaggaaacc
     2101 ggccccgctc cgtgggcaat ggggagaaac gcgtacgaag ccagtcggcg catcggagaa
     2161 cgcatggtgg aggacgaggt cacttggctg ggcaggaaag ccggcgaact cctggaggag
     2221 tacgaaaaat ccagaccatc gcacacactc agaacgtttg aggatgtcga acgcctctgg
     2281 gaaggtgttg ttcgccctca cgttcccgag ttccgttcca tgcaactctg ctggaaggaa
     2341 caccaagagg tgcccgggga cagcgtgtgg tactcaaatt ggaaggttcc cgagcgtgcg
     2401 tgattccatc ggggttgctc atgttttcgt tcgacgcccg cgggagacac ccgttcagac
     2461 gtcctctgga acacgttact ggcggtcccc tttcgccccg gggtgaaagg ggacattcgc
     2521 catccaggca ttcatttgcg atatttatgt ctctcgcggg cggccccggg atttcgtctt
     2581 tcatttccaa ctcattgcac gagaattgtc tatgacttca agtgaactcc gccgaggctt
     2641 tcttgactac ttcgccaaac ggggccatcg gatagtcccc tcgtcccctt tgatcccgca
     2701 cgatgatcct acgctgttgt ttgccaacgc agggatgaac cagttcaagg atatcttcct
     2761 cgggcagcgg gttccggaat tccgccgcgc agcctcctct cagaaatgca ttcgagcggg
     2821 aggaaagcat aatgatctgg aggacgtagg gaaggacggg acgcatcaca cctttttcga
     2881 gatgctcggg aactggtcgt tcggcgatta ctacaaagcg gaagcgatcg aatgggcgtg
     2941 ggagtacctg acctcggaac ttcaactgcc gaaagaccgt ctgcgggcaa ccgttcatca
     3001 ttcggacgat gaggccgagg aactctgggc gaagattgcc ggaatacctc gatcccatat
     3061 ctcccgccac ggtgacaaag acaatttctg ggaaatggcg gaagtcggcc cctgcggccc
     3121 ctgcacggaa attcacctcg acatgggcgc cgaccgcggg tgtggccgcc cggattgcgg
     3181 gccgaactgc aaatactgcg atgaggtgca cgacgcgagg ttcatggagc tatggaacct
     3241 cgtgtttatg caatactatc gcgatgaaaa tggtgttctg acgccactgc cggaaacgca
     3301 cgtagacacg ggcgcaggtt tcgagcgact ctgctcagtg atccagggca agagctccaa
     3361 ctacgacaca gacctgttca tgccgatcat tcgcgcaacg gaagagaaaa ccggccgcgg
     3421 atacgtctcg ggagtggaag gcacaccgtt ccgcatcatt gccgaccaca tgagggctct
     3481 cacgttggcc atcaccgatg gcggattccc gtccaacgtg ggaagaggat acgttctccg
     3541 ccgcatcctg cgccgcgcca gccgcgccgg tcgtcagttg ggattgcgtg aaccctttct
     3601 ctacgaactg acgaattcag tcgtcgacac gatgggaagc gcgtaccctg aactggaaga
     3661 gcgccgtacc cacgtcgcca cagtcatcaa acgtgaagag gagcagttcg ggcgtacgct
     3721 gaaccgtggc atcgatcagt tcgaaacgtt tgccgcggaa ctggcggcaa ccggatcgaa
     3781 agtactcgac ggcgagaagg tattccagct ttacgacacc tacggtgtgc ccgtcgacct
     3841 cactgcgctt atggctgagg atatgaagct gaccatcgac cttgacgggt tcaatcggtg
     3901 catggaagag caacgggaac gatcacgcgc tcaggcgaag ttcgatcgag ccgtcacgaa
     3961 tttcggcgag ggtctcgttt cggaattcgt cggttatgat accctttcga cggaggcaca
     4021 gataacgggt ctagcggaag gggccattgt actggaccag acgccatttt acgcggagtc
     4081 tggcggacag gtggctgatg taggggagtt gttcaacgcg gagttttcgt tcgaagtgac
     4141 gggggttcaa aaacagggaa ccgccatagt gcatttcgga aaggtgacgc gcggggcgcc
     4201 ctccgtcgga atggccgtaa ccgctcaggt aaacagtgat ttgcgcgggc gcaccatgcg
     4261 gaaccattcg gccactcatc ttctccaggc cgcgcttcgt gccgccctgg ggccgcaggt
     4321 tcatcaggcg gggtctttcg tatcgccaga ccggttgcgg ttcgatttca cgtgcgggga
     4381 agcggttccg cccgaggtat tgcgccaggt tgaagcaacg gtgaatgccc ggattcgtga
     4441 gaacctcgcc gtgaacatct cgcactcaac actcgacgaa gcgaaagcct caggagccat
     4501 cgccctgttc ggtgaaaaat acggtgaaac ggttcgcgtc gtgcgcatgg gcaacttcac
     4561 catggagctg tgcggaggaa cgcacgtgga cgccacggga cagattgggt acttccgaat
     4621 cctctccgag agtggcgtcg ccgcgggaac ccgaaggatc gaagcagtaa caggagtgac
     4681 ggcagaggaa ttggcccgag aggacagaga ccgtcttgca cgaatcagtg caattctcaa
     4741 agttggcgtg gacgacgtcg agccaagaat cacggcactg ctcgatcggc tgaaggaaat
     4801 cgagcgggag ctcgaaaaag ctcagacggc gatggcgggg gcggacgttg atgcgtggat
     4861 ccgcgaagcc ggtgaaatag gtgacgccag gctgctcatt cgagaggtca ccctcgcgga
     4921 cccgaaagcc ttccgtgccc tcggcgacac ggtgagagag aaactgggca aaggtgtggc
     4981 gctgctcatc gggcatgatg ccggcaagct ttccctgatg ctgctggtta ccgacaactt
     5041 gacctctcgc attcaagcgg gtaaactcat gaacgagctg gctgccatcg tcggagcgcg
     5101 cggcggcggc agagctcaca tggcacaggc cggaggaggc tctcccgagc gtctggcaga
     5161 cctcctggca aaggcacccg gggtggtagc ccgcgaactg ggcatataga gggaagggaa
     5221 gcaagaacgc tcttgcttct cgcgcaacgc cttggacatt atcccatcgc cgcccacagg
     5281 cgagtgcgtc cggcggcaac gggataacac cttcgccgag ggctccttat gtcaggcatt
     5341 gccttcgttc tcctgcttct ggggtcggca caggccgata ccatcctgtc tcgtggttct
     5401 tcaaacgagt ggtgctggcg ttgtcacggc atggcgacct tttccgtgct gcgggcggac
     5461 ggaaccattc gaacgctgtc ggcagattcg accgcgtttc tcgcctccaa tcatcggcgc
     5521 cttcgctgtg taacctgcca ctcgtgggaa gacgttcagg tgttccccca cgggaagaac
     5581 gcccacatgg cgcagacgtg taccgattgc cacgtggcgg aatcacatcc cctcaaccgg
     5641 acggacccgc ttcaggcaat gcactttcgg gaaatcactg acgagttccg aaaaagcgtc
     5701 cattaccaga ctgaagacca cgcgttcagt tgcttctcat gtcatgaccc acaccggttc
     5761 aggaagtctg aaaacccgtc gcttcaaacg attggaacac agaacggggt ttgtctcggg
     5821 tgccacggcg atgcggccag attcgccacg ctctcagaac gtcggataga cctgagcgcg
     5881 acgcacgcgt ggctgccgag acgggaactg cactgggagc atgtccgctg ccttgattgc
     5941 catacctcgt acgcacctcc gaatctctca cacaatatcc ttcccgcaga cagtgcagtg
     6001 catgcctgcg agcaatgcca caggcagaac tccatccttc tcgacaagtt gtaccgttac
     6061 cgccacgttg aggaaagaag caggaacggc tttgtcaacg cggcgctggt caacgattcc
     6121 tacgtcatcg gttccacacg aaaccggtat ctcgacctcc tcggcgcggt ccttgttggc
     6181 tgcgcatgcg tcggggtggc cgggcatgga ttcggccgct ggcacagcgc gagaaagaag
     6241 cggaagtgag gacgcagatg gacaccgtac accgcgagta ccttcacccc ctgtgggtgc
     6301 gcatatggca ctggttgaac gccgcgctgt tcctcgcctt ggcggtaagc gggttcaggc
     6361 tgcatttcag cggcagcgca gtccagttgc tgccttttcg taccgcgatc ctcgtgcaca
     6421 acgcctcagg tgcgctgttc acaattgccc tgatccttta cgccattctc ctcggaatca
     6481 ccggggaatg gcgtcagtac ttagggctac gcccgcggat gctcgcgctc gttgggcaga
     6541 accttcggtt ctacctcatc gacgtgttca aaggggctcc ccatcccgaa tcaccgacgc
     6601 gaactcaaaa gttcaacgcg ttgcagcagg ttacgtattt gttcgtgctt gtcgtcggca
     6661 tggtgcctgt tgccattacc ggcgtaatgc tgagcgtccc tgaaactgca ccgaagcaga
     6721 tcctcggagc ggggggaatc tggcctgttg ccgtgctgca tacattgctg gcgtatggac
     6781 tggttgcgtt ctttgtcggg cacgtctacc tgaccacgat gggtcccacg ctgtggagcg
     6841 acataaaaag catgttcacc ggatggcacg aacgtgttca cgggcaggct gatatccagc
     6901 cccaggaaca gggagacacg ccatgaccga cacccctcag cgaacctcga acgaatcatt
     6961 cccctgcccc ggatggtgga aacgtgtgtt ccggaaccga cggctgagga aatggagctt
     7021 cggtattctg gtcgccgttg tgtgcggtct cttcatccgc tatgtccttg tgccgtgggg
     7081 ttttcagaaa tacaccggtg tggtcaacgc ggagctttcc tatccgaaca gcaaagcagt
     7141 ttcggaggcg gagttcgaag caatcgcggc ggatcttcgt tccgaaggcg ttctccggcg
     7201 cgccttggcg ctcacgtcgt cggtaacgga ttcgattacg cggaaaaccc ggatcaggat
     7261 gatcgccgcc acaccgagtt tccaacggca ggaagaattc gcgcacattc gttacttcag
     7321 ggatgcgggt atccggcgtt atgagggccc caaaacgtgc atacgttgcc acgaaacgat
     7381 agatgtccgg aagccggacg gaacgattca gaaggtgaaa acgatccccg acgttttgaa
     7441 caccatccat tacaagtttc agagcatgga cggaggcttt acgacgtacg gttacgacgg
     7501 ccggcaggtc aacacaggca cccgcggtat cccggtcggg aagatagacc gcgcgtgcgg
     7561 agtccccggc agtttctcgt ggacaggctg ggctgcgctg attgaaacga agccggctca
     7621 cgccggcggg gaggtggtcc ttcgcagcga aggttgcggt cagtgccaca tcggaggcgg
     7681 gtaccatcct gcaaccgaaa acatgatgcc tggactcgtc gtaccgacgc cgaaggtcgc
     7741 ggcagaaggg ctcgattgcc tgatctgcca tgcatctgcc tatgacatga atcagcgctt
     7801 tgtgatcaga gatgaacacg gtctccggtg gaatcaggac aggtcactgc gggccgcgct
     7861 ctccgtcggc agaccgacaa acgaaaactg cctgaattgc caccagcaca acatgggtgg
     7921 agacacgtac gcctacaaca cctcggcaca gtcgctgggc acccagaatc agcgacttct
     7981 gcacagcact gccaagcgtg gcaatccgtt cagcgtgcat gatgatgtcc acgcgcgcgc
     8041 cgggcttgtg tgtctggatt gccaccgacc gcaggggcat aaaattcccc ggggaaacaa
     8101 gggaacggat ctggtggcga acgacctccc gggtgtcaag gttgaatgcg agatgtgtca
     8161 cacgtccgca ccccatgtcc ggaatcgcag gactcgtgcg gcgctgaatg gtcacgcaga
     8221 ttacatcgcc tgtgaaacct gccacatcgc tcgcctcctt cccttcaaca acgtgttgaa
     8281 agactgggtg catccggtct ggaacgagga agaaggcatg tacgttccga aggcggtgta
     8341 cagcagcggc gatcccaacc ggggtctcgc gtatctgtgg ttcaacggga acggaacgtt
     8401 tctcgcgaat gctctcggcg ccaaccccaa ccacagcccc gattacaacc cgctgatgcg
     8461 ccagatggtt gtgatagaag acccggtggt tctcggagaa atcgccgcga acacgcggga
     8521 tatccgaacg cgctacgggc tcgattcagc ttcgtacatg gcgcctatcg ccaatgcgct
     8581 atcgcaactc tcgccggaaa tgctttcgcg gcggcgggag atgattgaac gcaatcttcg
     8641 cgtccgtatg aatgaaggga agagcagaat ctatcccttc aagcttttca acgcgatgat
     8701 gtttgaggac ctgaacaacg agggcccgtt cggtgcaatg attctgccgt tcgactaccg
     8761 gacgtatttc gaaacagggg acgcggagaa cgccgtcaag gttgcggtcg cgaatcccat
     8821 cgtgaaacgg atgtatgaga ccccgttcaa gctctacatg atggacgaat tcatggcgta
     8881 tttcggggtt gggaagtgga ctgcgaaata cccgttggac gagggcaact ggaacgttca
     8941 acctcgctgg atgcgccaga tggggactct catggtgaac cacggcatcc agcccgtagg
     9001 caggcagtgc gccgagtgcc acaaccggaa cggtattctg gacttcgcgg cgctcggata
     9061 cacaacggac cgagtgcgcg cacttcagaa cctgccggaa ttgagctatt ttcagccacc
     9121 gttacgcccg tcccaccggc aggaaggcgc agaggttcag gcagaggcaa caaatgcttc
     9181 agaacgataa gatctgggta ggcaagggcg cgaatcccgt atacctgctg ccgaccatgg
     9241 caaatcggca cgggctcatt gcgggggcaa ccggcacggg gaaaaccgtt accctgaagg
     9301 tggttgcaga agcgttcagc gacatgggcg ttccggtgtt cgccgccgac atcaagggtg
     9361 atctggcagg gatagccgtt gccggaacac ctaaccccaa ggtggaagaa cggttgcgca
     9421 aactcggcgt cgaaggcttt cagtacgcgg gcttccccgt ccgtttctgg gacctgtttg
     9481 gcgaagcggg gcaccctgtg cgcgcaacgg tctccgacat gggcgcgctt ctgctttcga
     9541 gaatgcttgg gctgaacgat acgcaggcgg gggtgctgaa catcaccttt cgggtggcgg
     9601 atgacaacgg ccttctcctt ctcgatctcg cggatctgcg cgcaatgttg cagtacgtcg
     9661 gtgaccacgc aagcgaactt acgaccagat acggcaatgt ttccattcag agcgtgggag
     9721 caattctgcg tgctctgttg acactggagg atcagggggg cgcgaagttc ttcggtgagc
     9781 cggcgttgga catttctgac tggatgcagg tggacgcgca gggcaagggg tacatcaaca
     9841 tattgcacag cgtgaagttg tacatgtccc ccgcgctcta ttccacgttc cttctgtgga
     9901 tgttgtcaga gctgtttgaa gtgttacctg aggccggaga cctgaagaag cccaaaatgg
     9961 tcttcttctt cgacgaagca catctgttgt tcaaagacgc gcccaaggca cttctggaga
    10021 aaatccagca ggttgtccgt ctgattcggt ccaaaggcgt cggggtctac ttcgttaccc
    10081 aggttccctc tgacgttcca caagaggtcc ttggtcagct tggcaaccgc gtgcagcacg
    10141 cactacgtgc attcacgccg gctgaccaaa agtttgtgaa ggctgccgca agaagctttc
    10201 gcgccaaccc gagtctggat tccgagaaaa cgatcctgga acttggcaca ggcgaagcgc
    10261 tggtttcctt cctcgacgca gatggaaagc cttcgattgc cgaacgtgcg atgatcctgc
    10321 caccgcagag ttccttctcc gctatcgacg acagcacccg aagccgaatt atctccgctt
    10381 cgctgttggg ccgcaagtat gagaccgcgg tgaacaggaa atcagcattc gaaacgttgc
    10441 aggcggagtc gcaggcgccg cgtgaggcgc cacgagaacg gacgactacg ccgaaacgag
    10501 tgcccaccgg aaccaccgga cggagaacca gaaccccgct ggaagccgcc gcgggttcgg
    10561 tgatgacgac gatcggccgg gaggtgggca gacagattgt ccggggcatc ctcgggtcga
    10621 tactcaaacg gtaactcccg ggacgttttc gaaaatcgca gggccgggcg actggcgggt
    10681 tcagtcaacc gctccgccct cctccagaaa cacaagaatg gcggttgcat gctgaccaag
    10741 agaatcattc cctgcctcga cgtgcgagcg ggaaaagtca ccaaaggggt cgccttccag
    10801 gggaacatcg acgttggaga ccccgtggcc atggcaaagc attactacga acagggcgcg
    10861 gacgagatcg tcttttacga catcactgct tccaacgagc gacgggatat catgatcgat
    10921 gtcgtggcca gcgtagcacg agagatcttc atcccgttca gcgtgggcgg tggactgcga
    10981 acgctctccg acatgcgacg ggtgctcctt gccggggcgg aaaaggtcag tatcgattcg
    11041 ggagccgtca ggaacccgga cacgatacgc cagggggcgg aagccttcgg gcggcagtgc
    11101 atcgtgctat ccatgcaggt gaaaagggtc ccgcagacgc cgaccattcc ctcggggtat
    11161 gagatttaca ttgatggtgc gcgcacagcc accgggcttg acgcgatcga atgggcacaa
    11221 cgcggcgagg agcttggtgc gggggaactc gtggtgaact cgatcgacgc cgacggcgtc
    11281 aaaaccggct acgatgttga cctgaccaga cgcatggcgg aagccgtggg catcccggtg
    11341 atcgcctcag gcggcgcggg agagccttca cacctgtatg acgtgttctc tcaaggaaat
    11401 gctgacgcgg ccattgtcgc ctcaatgatc catttcggca cgtactccat tccggaaatc
    11461 aaggagtatt tgcaccggcg cggcctcccg atcaggatgg tgtgagcccc tgagcaatac
    11521 tgggagagaa aggcgaaagc gggctttttc tccgttggat ggcacatcat caaagcacgc
    11581 gcaggtggaa cccgccgtcc accacaatcg tgatgccggt agaatacgaa aatcggccct
    11641 ctgcgattgc gacaaccgcg tgcgccacat cctcgggttg tccccacctt ttgatgggca
    11701 gaaggtttcc ttcaagaatc agtttgtcgt accgttcccg tactttttcc gtcatatcgg
    11761 tctgaattac ccccggctga acctcgtaca caccgatgcc atccccggca agccgcgccg
    11821 cgaacacttt ggctgccatc gcaaggcccg ccttgctgat gcagtagtca ccgcgattca
    11881 cggagggtgc aaaggcgctg accgaggtta tgaacacgat tttcggctca cgaacgagcc
    11941 ccgcgcgctg ccattcgatc attttcgtcg ccacaatctg agtgaggaaa tagggaccct
    12001 tcagattcgt gcgaacaagc ctgtcaaagc tctcttcagt cgactccaga acatcggcgc
    12061 ggacttccgg cgcaataccc gcgttgttga cgagcagcgt caccggaccg agtcggtttt
    12121 ccgtttcggc aatcagctgt gcccggtcat ccgcatcagc gatatccgcg cggaccggca
    12181 tggcgtgccg cccgaggcgg ctgatttcct cacaggtttc cgccgcggct tccgcgttgt
    12241 gcgcgaagtt gaccgctacg tcgaaattcg ccttcgccag cccgagcgca attcctcttc
    12301 cgatcccacg cccggccccg gtcacaacgg ctacgggagg ttttgttgcc atgcgaactc
    12361 ctttcacgca gatcgtccgg catcaccagc agggaggtcc cgaaacgccc agtctgtgga
    12421 aggcgctcct ggcggcggcg ttgaccacca gaaccaactt ctgcaacgta gtcaactgaa
    12481 ccttcccgac cagcacggaa tgagtgttcc ggcttatctg gagcgctact tcgcgataca
    12541 cgctccccat caaggcaaca gccatccggg aatcgggaga gaccgcaccg caaagggggc
    12601 gggccagatc gaagtagcgg aacacgcgct cgagttcgaa ttccatcagt tgcagaaacc
    12661 cgggcgcagg ctcccgtgat ttcaggtcct cctccctgca cccgaaccga accaggtcct
    12721 cctgcggcac gtagattctc ccccggcgtg catcttccgc aacgtctcga aggatattcg
    12781 tcatctgcaa ggcaatgctc agttccgtcc cccagcgggg agtccgcggg tccgtccagc
    12841 cgaaaatcgc cagcgacagt tcactgatgg tggtggcgac gcggtcgcag tacaccagca
    12901 actcctcgaa cgttgcgtac cgcgttttga caaagtccat ccggcaccct tcaatgagcc
    12961 gcctgaacga ttcgagggga atgggaaaac gagctgccgc atctgccagt gccaccagga
    13021 tcgggtgatc ggtaactccc tgataacacc gttccagcgc tttttcccac gcgtccagca
    13081 gggcttcggg gttctcgtgt ccggtgtact cgtcggcaat gtcgtcagca aacctgcaga
    13141 acgcgtacac ggcgtacacc gcgtcgcgtt tgcgtttcgg aaggcagcgg aaaccaacgg
    13201 agaagttggc tccgtacagg tgcgtgattt tccgacaata gcggtaacac ccggccagat
    13261 cctccaaacg gggaatcgaa aatgatggag ccatggtcag gtgttaccct ttttgcaatc
    13321 tgcaccagac gcactgcgcg ccatgaatac aatcgggctg ctttttccac accgcccggg
    13381 caagcaccgc cagggcgcgt ttggcggcgt tgggtttgta gagtgcctgc cccacctcac
    13441 ggaagtccga acaagaagct ttggaaagga accagcggta cgcgagatgc ccgccggttt
    13501 cgtagagaaa acggttcgcc agccccattt cgaaacgctg cccgaaatgc tcctcccacg
    13561 ccggcccgaa agcccgtcct gaaagcacac actgtgcggc cagaacacca gattgcaggg
    13621 caagcctgat cccgaacccg aaaagatggt ctgcaaaccc tcccgcttca cccacgtgca
    13681 acgggcgcat gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacccccg
    13801 cttcctcggc ggcccgtttc aaggcgccat ccagtgcggt cggatcgggc ccccggtgaa
    13861 gcaggtaccc gaacggttcg gagctccgtc cgctcacgca tttccctgat gggccaaaca
    13921 gttgcgccga tcgctgcggc cagagcgaca cgggaaccga acccagcagg ctgcagagct
    13981 cggtccgtgc gtcctccaaa tccgggtagt tggtaagcaa ctggagattt ccccggaaat
    14041 ggccgcctac gtcaccgcgc aactcgcgta cttccacgct caaccctgca cgggcaagcg
    14101 ttatcgccga tgcaaggcca gcgggaccgg cccctgcgat gacaacgtgt tcctggttct
    14161 ccattgctat cccggatgac accaccgacg aacacaggag acatcgaaat cagattccgc
    14221 ctgcaggatt catttttccc cgagattccc gtccgcactc tcaaacggtc cagaggtgac
    14281 ccgcgggttg tgaacccttt cgccgcttct tcgaatgatt ctaggccgat cagcgcgcgc
    14341 aacgcaagac acgcgcgacg cgaccagacc aagaaatcag ccggcgtctt ccctcggaac
    14401 gcaaatgcac gcgtggccgt cgttcaagcg gtgaagatgc ttcagagttt ctgcgcgatg
    14461 agcattcatt ttgcggagac ggaaatacgc agacctcgcc atcggagggt tgaacagggg
    14521 ccgcaacggt gtgcggcggg tgaacatcac aaatcgcaga acgtcacgcg cccgcgtgca
    14581 actgctccac cgccttcttc aacgtgcttg cggaggaatg aagcgcaacc agttcacact
    14641 ccgaaaggac gctctccaca acccgctcga tgccacgcgc agatagaatg caaggcaccc
    14701 cgatacacac atcgttcagg ccgaattcac cctggagcag ggcggaaacg gtcaacacag
    14761 tccgctggtt ccgaagaatt gcgcccgcaa tccggacgag ggccaacccc accgcgaacc
    14821 acgtagcccc cttgtatccg ataatgtggt acgcggagtc cctcacctgc cgtgcaattg
    14881 cttcacgctc ggcaaggttg tcggcgcatc ccttacagat ggggcagtat gcgtccatct
    14941 gcatgccggc gatgtgagtg agagaccatg caacaaattc ggtgtcgccg tgttcgccaa
    15001 gaacaaaccc gtgaacgttg tggacgtcca ctccacaatg ggcactcagc agatgctcaa
    15061 gccgcgcact gtcgagaacc gtcccggagc cgataaccct tgagcggtcc caccccgagg
    15121 tttcgattgc gacatacgtc atcacgtcaa caggattcgt cacaacgagg atcactgccg
    15181 gggaagcctg ctcaatgatg tcggtcatca cgcctttgat gacggcagca ttcttctgga
    15241 gcagttgcag gcgtgtttct cccgggcgct gggccgcccc cgccgtgatc acgatcagac
    15301 tcgcggaggc gtagtcagaa gcgtcgccct tgcgaatgga aaccgtgggg aaaaagggtt
    15361 ggccgtgggc cagatccagc acctgaccgc gcacaagatc ctcgtttctg tcaatcaaaa
    15421 cgatttcatc ggcgagcccg ctctgggcca gagcgtacgc aaacgtcgcc ccgaccgctc
    15481 ccgcacccac cacaacgacc tttcgacggt ttcctgaggt atcctgcatc cgtgtcttcc
    15541 tcaatctggc gttaccttca cgagtccagg aaaatcggcg agcggcaaat tcgctttact
    15601 cgcccagctc acgggaacgt cgcgccgcag cctcgacagc ctgcatgagg gcgtttcgaa
    15661 tgcccgcggc ttccatggcg tggagcccgg cgatcgtcgt tccacccggc gatgttactc
    15721 tgtcccgcag cacggccgga tgctcttccg tctccaacac catccgtgcg gcgccgagca
    15781 cggtctgcgc agccagctcg agcgcgagcg gctttggcag cccgaccttc acgccaccgt
    15841 cggccagcgc ctcgataacc gtatacacat atccggggcc gctgccagac agccctgtta
    15901 ccgcatccaa ttgtttctcg tcaactcgga ctacccgccc gactgccgcc agtatccgtt
    15961 ccgcgcggtt catgtcggcg tctgttgcgt gcgcaccacg gcttatcgcc gccatcccct
    16021 cgccaatcag cgcaggggtg ttcggcatcg ctcgaacgac atgaacgggg tttgccgcct
    16081 catcgtcgcc agagagccat cgctccagct tgccggtcgg tacgccggcc attatggaga
    16141 gaaccgtctg cgtcggcatg agtttgccgg ccaccggctc gagcgcctcc gcagccacct
    16201 gaggcttgag ggcgatcacg accacatccg cgttttttgc ggcctcggtg ttgtcgttcg
    16261 tcgtcctgac gccactctct gcgtgcaaac gttcccgcgc cgacgcgatg acgtcagaaa
    16321 ccgtgatctc gctcgcatcc gcgacaccgg cgtggagtat tcctttgacc agcgccgtac
    16381 ccatctggcc gccaccgata attccaatcc ggagagtcgt gtccatgtca gcgctcctgt
    16441 cttttccgcg aactggttgt tttcattcca atggtaccac ggttgggcgc cgaacagaca
    16501 gggggaacgc gaaacacgcc ggcccttctg tggcattttc attcaatctt aacactcctt
    16561 ggacagatta tgaactcgtg gtatacatcg tacaacaaga gctgagggtt atgcgatgcc
    16621 gggacaacac gttgccatta tcgaagacga agacgacatc ctggaactca tttcgtacaa
    16681 cctgcgcaaa gaaggctacc gtgtaaccac cgccacaagc ggggaagcgg gtttgctggc
    16741 ggtcaggaac actcttccag accttgtgct gctcgatctg atgttacctg gcgtcgacgg
    16801 attcgaagtg tgcaggtcgt tgaaaaaatc ggccgaaacc tcgcacatac cgatcatcat
    16861 gctgacggca aaaggggaag agactgacgt agtggccggc cttgagctgg gagcggacga
    16921 ttacattacc aaaccgttca gcccgcgtgt cctgctcgcg cgcgtccggg ccattctgcg
    16981 ccgcaaaccc aaaacggagg acgagggctc gtccccgatc gtcgttcacg atgtggccat
    17041 tcatccgggc cgccatgagg taaccgttgg cggtgttcat gttgcgctta cacacaccga
    17101 attcaacctg ctccacctgc tcgcaagtcg gcccgggtgg gttttcacgc gctatcagat
    17161 cgtcgaggcc attcggggag acaattctcc ggttacagac cgctccgtgg acgttcacgt
    17221 cgtcggcttg aggaagaaac tcgggcaagc cggacgttat atcgaaacgg tgcggggaat
    17281 gggttaccgc ttcaaaggtt gaacgcatgc gccgcgtgac gtttgcatgg caccttgtgc
    17341 ccgtggtcgt tctcatcgtg tggggcgcgt cgctcctctc cgcgatgtat gccgcccgcg
    17401 ccatccgcac gttccacgcc gggcaaacgg gtgccgagct cgccgcgcag gcaaaattgg
    17461 tcagacaggt gattaccccg tttctcgtcc aggcgaatcc tgcttcagtc gatagcatct
    17521 gcaaggtttt tggggttgcc tcttcttctc gaatcacggt ggtaagcaag tccggcacag
    17581 ttctgggaga aaccggtccg atcgcggcac ggcttgataa ccacgccgac cggccggaaa
    17641 tcatcgcagc attttccgga aggactggct ctgcggtacg attcagccac actacacgcc
    17701 ggacgatgat gtacacggcg attccggtgt ttcagggtgg tgaaccggtg gctgcggttc
    17761 gcgtggcgaa gcccattgac agcatcgaat ttctggttgt tcacctccag tggcgtttct
    17821 ttctcgcagg tgccgtggtg gccctcgtag ccactgtcgc cgttctgata gccatccgaa
    17881 gggcaaccga tccgctggag acgatgcggc gggtcgccca cgcgtattcg cggggggatt
    17941 ttcgcgaaag ccttccaaca tcacgcgtgt ctgaggttga cgaccttgcc caatcgctca
    18001 acgcgatggc cagacaactc gatgagcgga tacgctcgct ggttcgtcag cggaatgagc
    18061 aggaggccgt tctttccagc atggtcgaag gcgtcatcgc ggtagactcg gaagagcgcg
    18121 tgatcaacct gaaccttgcg gcggcaaggc tcgtgggagc agacattgcg cgatcgcatg
    18181 gacgattgat ccaggaagtg gtgcggaaca ctgagcttca gcagataatt cgaagagccc
    18241 tcgacagcca cgaaccggtt gagggggaaa tcgccatccg gtcggaaccg cgggtcttcc
    18301 tccaggcgca cggaaccgta cttcgcgacg ggacaggtga atgtatcggt gctctcgtcg
    18361 ttctgaacga catcacccgc ctgcgcacac tcgagaatat gcgaaaagag ttcgtagcca
    18421 acgtttcaca cgaactgaag acccctatca cctccatcaa ggcctcggtt gaaacgcttc
    18481 tcgatggcgc gatggaaaac cgggagatgg cgagaaggtt cctcgacatc gtggtgcggc
    18541 atgccgacag gctgaatgcg atagtagacg acctgttgag tctctctcga atcgaacagg
    18601 aagcggagaa ccggcagata gagttggatg ccctcgatgt ggagccggta ttgcaggccg
    18661 cggtccagtc atgcctcgta actgcagatg aacgtggaat aaacatccag gtctcatgtg
    18721 caaatgatct caaagcaaga atgaacgctc ccctgatcga acaggccgtg gtgaacctga
    18781 tcgataacgc gacgaaatac agcgaccctg gcggggtcgt gcacgtcgtg gcggaacaga
    18841 agggcacaga agtcaggatc gatgtccggg ataccgggtg cggaatcgcg cctgaacacc
    18901 tgcagagagt cttcgagcga ttttaccggg tggacaaagc aagaagtcgg aacgcgggcg
    18961 gaacgggact tggcctttcg atcgtcaagc acatcgtaca ggctcacgga ggtcgcgtga
    19021 cggtggaaag tgcattggga aaaggcagta cgttctccct gtttcttccc gctgcataga
    19081 ctgaaagagg ctggcaacac ccctcaaagc accaccatgg ccttgattgc cacccggcga
    19141 gtccacgttt ctgcgctcgg atcacccacg atcagaaaca gatcgaatcc attccgcaca
    19201 agctggcggt agctgcagta gccgttgaaa cggctcttcc atgcgttgcc ggagccgtcg
    19261 tcgttgtacg tgagtctgcc cgaaatgctt cgttcagcag tcaggtcata ctgcacggtc
    19321 gtcaccacct ggtaacggtc ttccacacct ccggtcggtc gtaatgcgtg tcccgcaggg
    19381 aaatctcttc tgcgaaactg gaggcgcgcg ctcgcggtga cgcgatccca cgagtggata
    19441 ccctgatcga gggtcacaag caggtagtca gccccctgaa gccatcccca ccgggccgaa
    19501 atccctccct tctggtaggt ctggtcctgc ttccacccaa actcatggag aaacgtccaa
    19561 tcgcggtacg gcgggcgttc ttcaacccgg aactccgtgg ataactccgt tcctccccgt
    19621 gctttcgtcc acgattcgac ggtatagcgc tggagataga ggttgccgtt gctgcggacg
    19681 gaccggtgaa cgaacccgga tacgttccac tcgcggaacc actgccggtc ggccgtgcgc
    19741 tcgtagccca gctcgatgga agcgtcgcgc tggtcgaagt ctatcgggtc gagcagcccg
    19801 tcaaccggcg tcattccgtt gcccaggtgg cgggccttca ccaccagcga cggcccactt
    19861 gcggtcggaa taatctggta atccccttcc aggttgaatc cgtccgcgct gtccccatca
    19921 ctctgggcaa aacctgtctg caggcgcatg tattcggtca agctcccgat catgcgagat
    19981 ttcttcacca gcgagacaaa aagcaaattg tcttcgacca ttggcacgcc ggagcgcacc
    20041 ggcgtattcg gaggtgcatg gcgccatacc acgttgttct cgaattccaa cctcggaaca
    20101 ggctgccagt accaccgggc ggcggaatgg aggacctcct cgcggtcgta cactgtcagt
    20161 acgccgatgc ggtggttgcg gatttgcccg aaatacttcc caccgccgta cacctctccg
    20221 atgcgttgac cgtacatcat ccacgcatct ggcaggaacc ggtatccctc aacgaaaaag
    20281 gggcggctgt cagcgcgttg cttttcgctg tacgagaagt ccagcccgag aacgtcgttt
    20341 tccacatttc gaaaatcggg atacacacac ccgataaacg tctgaccgga ggggaaggtc
    20401 ttcttaacgt ccaaccctac gtacccatca aaacgcccgt cacgcgcctg agcaacgccg
    20461 tacggcatca ccagtgggaa agcccggccc gtcgagccca cgtcgaggtt ctcccagtgc
    20521 gcgattcgcg ttcgatccca tgcgaatccc atgttcggcc agcggctttc ttcctgcagg
    20581 cggggcaccc acctccggat cgaaacgccc accgtgtttt tccccgaagg gagtccgagc
    20641 atgctgtacg gcacggaaac ctccacggtc cagccagcgg agtcgatgcg agcggccgcg
    20701 gtccagtccc cgcgccattc gatctttgtc gctgcgccat ccggaatgaa atcggcctgc
    20761 gttccgcgag gggtaactgt gaacgcgtac gcgccattac ttcgccggag gttttccgcg
    20821 tcgatttcga acgagatgaa atcgtcgctc agcgaaggca ccgcattccc tttgtaggcc
    20881 agttgcagcc gctttcgctc ttccatgcgc agcttgtcgg gattcctgtc aaggcaccgc
    20941 gcgaagaaat agaggccgcg gtcatcagag gccatccaca ctacagtgga ttcggtcgga
    21001 gcccggttcc agtcgtggca gtggaagtca tcgacacgaa tcgcgcccga ccattccgcc
    21061 ggggtccaga tgccgtcaat tcggggaggt accgccaggc ggcccggtgg aatgcgcaac
    21121 gcattggcgg gggcacccgc tgcgggaatc agctccccgg caagcgaacg cgatgacgcc
    21181 agaaggagta caaggaggac gaagccggaa atggaacgga acactacgca ggagaaatgc
    21241 cctgtcaaaa atgtcaccct cacctcgatt gcgggcgtct cgaaaccccg gcatcgtcct
    21301 tcgcgacgcc gaagccctgc ccggatttgt caggctgcca tgccgtttgt aaaaattcgg
    21361 acccgtcgtc gggacgcccc aacccgtctc gcaccgcgag ggcccgttga ggtgattttg
    21421 aaaaccggtt gtccattcgt ttctacgtgt cggtgttcgc gatcctcgct gtggttcacg
    21481 ctctctaccg cgaagcgagg cacaccacgc cgcgtcgtcc ccgcgtgctt ccacacctca
    21541 ccgcatcgta aattcagacc gatccctttg gatatccctt gacttcttca gggaactccc
    21601 gatgacgttc agccggaatc ccgccttcat tgcggcaatt atcctgctcg ccctgcaggg
    21661 atgtgaaagg acacgggtca caccacccgt tgagatgcgg aacctgcgcg tgactcccac
    21721 gaaaatcggc ctcgacataa ctttcgagtt gaccgactcc aggggtcaac caacgatcat
    21781 gatgttcggg aacggtgtcg tgcggattga cgattcgccg gacagcgtcc agaaagaggt
    21841 cttccccggg catccggcgg ggagctcagt cctcgattcg acgtggctgt tgtacaacgc
    21901 acgccttttt gtaacgcgcg agcattttgt gatccagcga ggcggggatt cgacacgcgg
    21961 gcaagacaaa tacctctgcg cgctgggagt gtacccctat tcccatttcc tgaggaagcc
    22021 gaggcgcccc acgggcaacg tgcgggtagc gctggtggga atggatggca gacgcgttct
    22081 tctggggcac gcacccgtta tctggccatc tgaagcattc agcgcgacat caggaaccgg
    22141 cgaccagagc cgcttcggca atcgttgacc agacctgcgc ttccgggccc cctgcacctc
    22201 acgccggcac gatcttgccg agtacccgac gccccctcgc tccagtcgcg cccggtacat
    22261 tggaaggttc gccgcagacc gtctcgttgg cgagaaccgc gaacgcgatg gcttccttcc
    22321 cgtccaccgg caaacccaga accgacgagt cccccaccgg taccggggcg agcagctcgg
    22381 ttatccgccg cctcaacacc gggttccttg cccctcctcc cgaaaggtac acggacgaca
    22441 gcggaccgtg gggaagcacg aacctcgcgt acgcatccgc aatggaccgg gccacgaatt
    22501 cagtaagggt gctcaccagc acagcgtccg gacgcacccc cctgcgctcg accacgccac
    22561 gcacgaactg cctgccccac tgctccctcc cggtactttt cgggggcggt tgccggaaat
    22621 agtcgtcgtc caacagatcc cgaaccagac cttcatccgg gcgcgcactc gcggcgattc
    22681 ttccatcctc gtcatacgtc ctcgatccac gggtcatcaa agtcaccgcc ccgtcaatca
    22741 ccatgttccc cggccccgtg tcaaacgcgc ggacatcctc cagccgcggc gtcaccacag
    22801 tgagatttgc gattcccccg atgttctgaa gggcaatcgc ctcctcgttc cgccggcaca
    22861 gcacccaatc cgcatatgga acaagcggcg ctccctcgcc tccggtggcg atatcccgcg
    22921 gtcggaagtc tcctatcgtg gtgatacccg tgcgctctgc gattacggcc ggctcaccaa
    22981 tttgaagaga ggaggccgtg cgcccctctc ctcgtggcac gtggtacacc gtctgcccgt
    23041 gagagcctat cagatggaca tccgccggcg caaatccggc agatcggatc acgttgagtg
    23101 ctgcctcggc gaaaagctcc ccgagcgcaa agttcatctg gcacagttca cgcacgtggc
    23161 cgtcaaaaag agcatgaatc tgctcctgca cctcgtgcgg aaacgaaaag gtctctgccg
    23221 cacgcaccct ctccagacgc agagccgtac catgcccgct gatttccacc agcgccgcat
    23281 ctatcccgtc cacggaagtc cctgacatca ggccgacaat gcgtcgcacc tgcagcctgc
    23341 tgatttcctc tggcgacact gcaggcacct ttcacagagg gtgatcgagt tccgaaagtg
    23401 cgagaaacaa cgcattccat tcactcaggc gcgccggaat gtcttgcgcc gtccacgggg
    23461 tcgtcacgag ccgctgaagg gttcggcgcc catctcgcgg cgcgatgggc aaggggagca
    23521 ctgccgcctc cacccatcgc ggaaacgcgt ggacacgacc gttcaacccc gcccaggcgc
    23581 gaatcagata ctccagcccc ggcccgagag tccgcaccat agcttctgca gcagacccgg
    23641 cggacttcac agttgcctca tcccattccc gcaacgcact cagcgagacg gtggcgattt
    23701 cccgcgcacg ttgtgcgagc agatcggaac ggcggacaag gccggtggct atcccttggg
    23761 gatccagaat gatctggttg gtctgaagca attcaagagc ctcaatcgct ccacccggaa
    23821 tgaatggccc ctgcagcgcc acttcgagcg accggatcgt atggaaccga aggtccacac
    23881 gccgccctgc gatcaccatc tgatccgcat ggataacgtc gtcccccggg tattgccgga
    23941 ctatcccgcg atccgccagt ggcgcgaatg cccgaagcct gctgcgagga tccgggatct
    24001 cgtgtgcgag cagccgcaca tccaggtccg atgcgcggtc ggcgcacccg cgcgcgtacg
    24061 aatccgctac gagaaccgcc accgccccca gcggcttcgc gcggacaaaa aacgcctctg
    24121 ctgatgcatc cacctcgcgc ggcgttaacg ggaggcctcg aaccgcgcct tcggcgaatc
    24181 gtagtgacgt tgcggcagtc ccgagggcgg tcagtgactc cacggcctcg ttcacaagct
    24241 cttccgccag agttgccgaa cgttccctgt ctgcaagcga taccatcgtc tgcacgcgtt
    24301 ccgcgaagcc gtccctcacc aacaccgag
//
LOCUS       DAOSOO010000024        24322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2080_length_24322_cov_5.643590, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000024 DAOSOO010000000
VERSION     DAOSOO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24322
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_24322_cov_5.643590"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLT86_03170"
     CDS             <1..234
                     /locus_tag="PLT86_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT35648.1"
                     /translation="YGAAGLLQLQQHGLLDELQIVVSLVTITLFVPCVANVFMIAKER
                     GIRTAIWMVVFIFPFAFLVGGLVRWGMILTGSG"
     gene            458..1141
                     /locus_tag="PLT86_03175"
     CDS             458..1141
                     /locus_tag="PLT86_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT35649.1"
                     /translation="MGRHEMKHLPRECENILVQVWSAHEKGDKSLGRLFLAHPHAVPV
                     VDELLRAGLLERLDGEIWLTERGAKAARELVRRNRLAEVLLQQILEVQPERVAVNACA
                     WEHVLSAEVTDTICTFLGHPRHCPHGNTIPPGPCCAKSSRTVTPLVQPLTTLPVGGTA
                     RITFIAPRVSRRLDRLAALGVVPGTKLVLKQKSPAYVIRADQTDIAIEEDVAKDIFVR
                     EIKAEEEGG"
     gene            complement(1164..2192)
                     /locus_tag="PLT86_03180"
     CDS             complement(1164..2192)
                     /locus_tag="PLT86_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35650.1"
                     /translation="MKNIDVSRLVNLGVVPARRFFAATDSLAELTASHGRRTFLAAFP
                     SDRVLSIGLYQDAEIDLEEAATRYPWPMLRRSVGGHEMSVVPGTLLFSMIIPQDDAEN
                     SITRACRGMVPSLVATCRSYGVDAHFTPPDSVRYHRILFSAVYAGCVGNALVLTGTLP
                     CTSEEPGGRNAVEVSLSGISKGAVGPAEVLDVFVAKLGEQCRTEFREERLSPAEEAAI
                     NQKVLAGSESEPRRKPQRAELRERARRDPRLDLREAQFTTAGGRTIGLSLLLRGEVIE
                     TVAFSGDFVFYRDCLSDLETALHGVPAEWDELMTVLENYYIINQVDSPGVTPADWVSA
                     IDAATGML"
     gene            complement(2300..3226)
                     /locus_tag="PLT86_03185"
     CDS             complement(2300..3226)
                     /locus_tag="PLT86_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT35651.1"
                     /translation="MQPLEFRPDGLFVLVQFTDTHFADLPHNRETFELFDRVLQIEKP
                     DLIVLSGDIVHPAWCKDPLIHWADVVRYFDAKNVPWAFVQGNHDAQEFTYEAIEDTLA
                     GSRLSLYESGPESIFGHGNYAVPIYKRGTSRPGAIVWSLDSGQGAEGVASGWDWVKRD
                     QIAWFSAEADRLMKQDGDSITGLAFVHMPLVQHLTVWQTTPCSGFLNEKVCMQGMDEG
                     LFDAFKERSRVAGCFVGHDHVNDYEGVLEGVTLAYGRGSGYNCYGKEGFRKGARVITL
                     REGVRGFQTHIRLDDGTLAPRPSHLPDGTSLN"
     gene            complement(3358..3648)
                     /locus_tag="PLT86_03190"
     CDS             complement(3358..3648)
                     /locus_tag="PLT86_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35652.1"
                     /translation="MANKKSDPVPPEEIEITSQGTESAEAEATAPVEQDAQTKTATEQ
                     EAEEQPEGVMAADKDFEENVPCAAFPWKEVVKWSIVVTVVALVLYLIFRRDD"
     gene            complement(3775..4317)
                     /locus_tag="PLT86_03195"
     CDS             complement(3775..4317)
                     /locus_tag="PLT86_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOT35653.1"
                     /translation="MTISKDALNDEVFRVGVLSDTHGRLNGAVFTLFEGVDCIFHAGD
                     VGKSQVIRELELIAPVHAIRGNTDPFDLPFPEVLDTSHKGIRFHVRHVVPTSGRSLEF
                     FTMRMDADVVLFGHTHVPFLEKVRGTLFLNPGAISRSRVPAETAAVITVRGGEIAATV
                     HDLVAHEFPVRVRYPAAAGA"
     gene            complement(4322..5740)
                     /locus_tag="PLT86_03200"
     CDS             complement(4322..5740)
                     /locus_tag="PLT86_03200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOT35654.1"
                     /translation="MDWRYERDSLGEVKVPAGALYGAQTQRAIENFPVSGIRLPRSFI
                     RALGLIKYAAADVNEQLGYLDARIADVIRAAALEVAEGKLDEHFPVDVYQTGSGTSTN
                     MNANEVIANRAIQMLGGVIGSKTPIHPNDHVNMSQSSNDVIPTAVHVSAHLKVVEELL
                     PALHHLADTLSHRAEENDDVVKTGRTHLMDAMPIRFGQQLRGYQTQVSKGVKRIEATL
                     PRLAELAIGGTAVGTGINADKAFAGGVAEKLSELTGIRFRETDNHFEAQSAQDAAVEL
                     SGQIRTVAVSLMKIANDFRWMNSGPQAGLGEIVLPAVQPGSSIMPGKVNPVICEATTL
                     ACAQVMGNDLAIAIGGQSGNFELSLMLPLIAHNLLQSISLLANVSRLLADKALTGFRV
                     NARRMTDLVEMNPILVTALAPRIGYDLAAHIAKRAMAEGRKVRDVAREVTDLSEAELD
                     QLLDVRAMTEGGFTGEQTGSSG"
     gene            complement(5765..7732)
                     /locus_tag="PLT86_03205"
     CDS             complement(5765..7732)
                     /locus_tag="PLT86_03205"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT35655.1"
                     /translation="MPSTTANGAIWQITSNNITVHIDTVTMTLTMQRSGGGPWRMVET
                     SHDLTLALNGHETPVALASADQKQATLYEAGELSGVRVVLRGFPAGNRSVDVSLAIIV
                     AIDGVSDELVVRVIPAHDPQRVVREVRYPRPFVLEGRGPGVHHVLPSMQGALLPTDWP
                     GKIDWWWIGLNWTRAIYMPWWGAIRPGDAYMGILETPFDGGLHFSHPPGGPTEVGPKW
                     HPSMGSLSYLRQVRYHLFGSVDHNALVAKYREYARTIGRLKTLETKATEVPEVKLLQG
                     TTVATGYTLYHIQPDSHYYHPDDPGKNHNLRTFEESIRGVERLREIYPTDRLVYHLDG
                     WGTRGYDNLHPDYLPPCEEAGGWDGFKKLAEAVDRLGWLFTTHDNYIDYYVDAPTYRE
                     YLARVAEDGSVPGKAWWYGGAQTFLCEKNALGFVRRNFEEILRRRVPLRATYLDVFSI
                     IEMLECYHPDHPMTREQSLQARAECFEWVRSRGIVLSSEEPTDWSVPYIDFCYWAPVA
                     MADESPDAPTFGIPVPLFNQTYHDCVVTPAPLGKSGAVSEKDAFLYALLYGCPPIVAA
                     PEPKVQSGPGAPVESAARSAHFHRAKILADVHRQTGFAPIEAHDLLNTEGTARVTRFT
                     GGASIEGDFTAGKYRIVGLKGIDDTWQVVPD"
     gene            7915..9006
                     /locus_tag="PLT86_03210"
     CDS             7915..9006
                     /locus_tag="PLT86_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35656.1"
                     /translation="MTSRERLRAIFAHEPYDHFGIFEHFWGETIPEWRKQGFPEDGDP
                     ARFFDYDLRGVGGGPNATPFRDFSELIQETDEWKVTRSGWGAALKHWKHHSGTPEHID
                     FVIKDATTWRPYRDQLVGPADPTRLDIDGTRKNWKEAAAEEKYCTFSCLFIVETLRHA
                     LGDVHMLESFILDKPWLHDVNRVITDFYIRHFDLLFKEVGVPDGIFIYEDLGFTNGLF
                     ASPKTLEELIFPYYREFVDFFKGKGAQVLLHSCGGVTEAVPMILEAGFDCLQPMEAKA
                     GVDVVALARKFGNRIAFMGNIDVTKLNTNDRAIVKAEVLGKLNALYDLGAGYVFHSDH
                     SIPPDVTFDTYRYAVDLYRDFCATHPCGA"
     gene            9017..9610
                     /locus_tag="PLT86_03215"
     CDS             9017..9610
                     /locus_tag="PLT86_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT35657.1"
                     /translation="MNGEPEYYDIRQSAFQTWFEKGLPYEDYLARSDPRHAERWRTAE
                     KAIALTAEQRRLLGSFVRQMPVLCLSGVWCGDCVRQGPILHAFEKAAPVVNVRFLDRD
                     EFPDLMDELRICGAKKIPVVVFLTEDFFEVQRYGDRTLSVYRRKAERELGPACPVTIG
                     PQDGNEAAVEIQEWLDTFERVQLMLRLSPPLRRRHGD"
     gene            complement(9681..10604)
                     /locus_tag="PLT86_03220"
     CDS             complement(9681..10604)
                     /locus_tag="PLT86_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOT35658.1"
                     /translation="MAASELIKANLLHLSYNMWADREDPERPDSYTTARPYLRFDESL
                     WRDILRKMVENGLNMVIIDLGDGIRYESHPEIAVRGAWSPQKLREELKFIRGLGLEPI
                     PKLNFSACHDVWLGPYSRCLSTDTYYAVCADLIEEVIRLFDTPRFFHLGMDEETANHQ
                     RRFEYVVIRQFDLWWHDFLFYVEQVEKAGVRAWIWSDYIWNNREVFLKRMPKSVLQSN
                     WYYDVVFNDTVNYVKAYHDLEEHGYDQVPTGSNWSHPDNFGMTVEYCRKIIAPERLKG
                     FLQTPWKPTLEAERKRHVEAVEQVGRALRGL"
     gene            complement(10586..12385)
                     /locus_tag="PLT86_03225"
     CDS             complement(10586..12385)
                     /locus_tag="PLT86_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT35659.1"
                     /translation="MVAYHVLPAIRTCRNLVVQGAICLLFGVMCGCAPRETAPEQTVD
                     ETRTIYFATHMGEHPGWIPAFAALARRYEELHPGWKVRLHYQPLDGYQVWAETQFISG
                     RGPDILDFGGGDAFRFGARMGLLVPLTEQLQEESPYAPGKRWKDTFYPIVWKNAEDPI
                     YGELWWVPFNFFTVRIFYNKELFARAGITQPPTSWKEFVAVQQKLLDAGIVPYLMPNS
                     NNNPHKYIFTCMEFLSDMMFQDRVPEIDTVIRDGQVDGIESNISIYKGLTRLDDPQML
                     AVLHLLKEWSRYWVRGYNGLDRQQSKMLFANGIGAMYMDGSWETEGLAEAVDFDWGVF
                     PMPLVTKETTPYGDGPRNDGSYNLSFSIGKVAERRGHLAQVVDFLQFLTSPEAADTLR
                     NYVSFISALEDYPVPDDLKSFAPASGYPWSYNAWAVSPQSASMEGGDVLFSLLQGYCA
                     GSISERDLLERYGKTYRELTVEELRFALRENAQRVLEGRRRLTATRAVRDSLARVLGA
                     DPDSTTATCAEFARLNRHVAFQEESLRRANLQFSALLETLSRPPAWERSLHTSGTGTQ
                     PALLSHSPCRSTPDPFRFPTTSVEGAQQWLHQN"
     gene            12551..13690
                     /gene="apbC"
                     /locus_tag="PLT86_03230"
     CDS             12551..13690
                     /gene="apbC"
                     /locus_tag="PLT86_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HOT35660.1"
                     /translation="MPEVTKEMVVEALSKVNDPELHRDLVSLGMIRDVAVTDGAVKFT
                     LALTTMACPLKSKMQEDCKTAVMRIPGVREVTIDLTADTPRNARPASGKKVGDIVLQD
                     MLPTVKNAIAVASGKGGVGKSTVSVNIAAALAMEGARVGLLDADIYGPSIPIMMGVTE
                     QPEITDSEEIIPIRKYGIELMSIGFVIPEEQSVIWRGPMVAKMLQQFLSGVKWGELDY
                     LIVDLPPGTGDAQLTLTQSVPLAGGLIVSTPQAVALADVRRGITMFKKVDVPILGVVE
                     NMSTFVCPKCGHEEHIFAYGGARSEAEKLGVPFLGEIPIDLSIREGGDSGVPVVVAHP
                     DSPVGLAFRKLARDAAAAVSVSNLTARKAERHEPARTGKELPMMK"
     gene            complement(13767..15476)
                     /locus_tag="PLT86_03235"
     CDS             complement(13767..15476)
                     /locus_tag="PLT86_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35661.1"
                     /translation="MSGHLKALSVVTSCLLLNTSLPLAVRAGVPDPRTTILVGVWGYP
                     PAPDDLADPFPRFTGSGTPDWFVANYRALKVNYYSYDSIKAAGIDMIMTPLGVNLQNE
                     GHCFATVRAANAARLFCLPLHPDVYGQRESDHLDVARVRRALSNPVWATPEDSSVIGW
                     YICDEPQNDREVDKALRLDSLLRAQLTEIGPLAGKDWLTFLGWFVWNDSLRIARILRG
                     IGTTGAGGPAVIGHEAFRFMNPDTAENPQIYPMDWLNASLARFYRTVRDNAPGTPWWS
                     VTHVTDEFGMGGPCVWYPYGVIDEASLRMDVFTRLGWGAKGIVYLTYSHYIDAIVLPD
                     YENCRPTGRTRVYDGRYWGADWADRHHTRRYARFPGVFTSEWSSETMSYRPTGFGAIY
                     RHIRAVNADVARIRPYLGPAEIFHPVMSEPPAVGGPYPTRSFFVDGVSAWEPDSVMRP
                     FKSGWGERDKAAVLVTPLRNGRDHYLLIVNQNIRYREGTVFESIRTGEAGDSAFVTVR
                     INAELIGGGGGYVAKNLVTGSITKKLIPSGHFVTLFDTLDTGEFRMYQIMKDTSSHVG
                     RLR"
     gene            complement(15585..16769)
                     /locus_tag="PLT86_03240"
     CDS             complement(15585..16769)
                     /locus_tag="PLT86_03240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT35662.1"
                     /translation="MGIAKTIATNLEQSSWIRRMFEEGIRLKAERGPEHVYDFTLGNP
                     DIEPPPEVIAVLERIVARNRPRSHAYMPNAGFPEVRAVIARKLARRTGLAYTADHILM
                     TAGAAGAINVVLKSLLDPGDEVIVLAPYFAEYRFYIENHGGVMVVVPTDDAFMPDTRR
                     IRAAITSRTKAILINTPNNPTGAVYGPGVLRSLNELIEEANHPITIVSDEPYVALTFD
                     GVEPPETASLISRSVVTYSWSKALAIPGERIGYIAISPLLPEAADLFDACTFANRVLG
                     FVNASAIWQLVVAEAGDVRVDATPYQEKRDFLWDALTRMGYEIVKPGGAFYLFPKSPL
                     PDDVAFIRLLQKEGILAVPGSGFGTPGYFRLSLTVPKEMMERSLPGFERTLHAAEGNG
                     KS"
     gene            complement(16783..18111)
                     /locus_tag="PLT86_03245"
     CDS             complement(16783..18111)
                     /locus_tag="PLT86_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT35663.1"
                     /translation="MAEFLFENRIWDPAETLPRNELQALQVHRLRDCVARLANVPFYR
                     KAFAEGNVTADRIRSLDDLKRLPLTTKADLREHYPLGFLATPRSEIVRIHGSTGTTGK
                     PTFVAYTRDDLALWANLCARFLVAGGLRPDHMVHIAFGYGLFTGGFGLHYGAELVGAA
                     IVPAAAGNTERQIVLINDLQADVLVCTPSYALNIAEMMRAQGYTPDKIPLKFAHFGGE
                     PWTEDMRLEIERQMGLLAFNNYGLSEVIGPGVSGECVVRNGMHIQEDHFLVECIDPDT
                     LEPVPYGEPGELVLTALTKQAMPILRYRTRDIARLDPTPCPCGRTGIRMGRVIGRSDD
                     MLIIRGANVFPSQIEEALLRVEGTAPHYLIEVDRPGTLDEVTVRVELRPERFSDKMSQ
                     MHVLREDIERQIHIVAGIRANVELVAPQTLERFIGKAKRVVDHRKEKGLI"
     gene            complement(18167..18598)
                     /locus_tag="PLT86_03250"
     CDS             complement(18167..18598)
                     /locus_tag="PLT86_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT35664.1"
                     /translation="MKIHQLSLFLENKPGQLTAPCRELADAGIDIRTLSLADTQQFGI
                     LRLIVSDWKRGYELLRDKGYVVKDTEVVAIEVPDRPGGLLEVLKVFEKSAVNIEYMYA
                     FTFGRGDKAVLIFRFDDPDAAIALLNSAGINVVGSVEVYNR"
     gene            complement(18633..19967)
                     /locus_tag="PLT86_03255"
     CDS             complement(18633..19967)
                     /locus_tag="PLT86_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT35665.1"
                     /translation="MTKTAGHGLGGFNPVSAPDYLPVPLLRDLQLARLQQIVRRAYEN
                     VGHFRKQMDARDLNPDSIKSLQDISKLPFTVKNDLRDTYPFGLFASPMGDIVRLHASS
                     GTTGKPIVVAYTKDDVEVWTSAMMRAFAACGVGSGDIIQNAYGYGLFTGGLGAHYGGE
                     ALGATVIPISGGNTDRQIMVMKDFGVTAIACTPSYFLHLLERASELGVDFHDLRVRVG
                     IFGAEPWSETMRRRIEEMSGIKAYDIYGLSEIVGPGVGIECSCQCGLHIFEDHFYPEI
                     IDPVTNAPCAEGEEGELVLTTLSKQAMPMIRYRTRDITSFVEGPCACGRTLRRIKRIG
                     RRADDMFIIRGVNVFPSQIESALLAVEGTLPHYQIVLTREKGLDQMEVQVEVTAEVFS
                     DRIGALEALHTKLESELEHTLGIRAEVRLVEPHAIQRSEGKAKRVIDKRNLG"
     gene            complement(19996..20490)
                     /locus_tag="PLT86_03260"
     CDS             complement(19996..20490)
                     /locus_tag="PLT86_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT35666.1"
                     /translation="MPTGSDVVNVVIAGLGGQGVIKASDILADVAFRAGYDVKKSELH
                     GMSQRGGSVSSDVRFGKKVWSPMVPFGCADYLVVLSSDQLEVNRHVLKEGGVTLSAEL
                     VDVSRLASPRCINVALLGALSAHLEFPEELWNAAIRSGFPEKLHDMNITAFQFGRTCT
                     KTQQ"
     gene            complement(20474..22102)
                     /locus_tag="PLT86_03265"
     CDS             complement(20474..22102)
                     /locus_tag="PLT86_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT35667.1"
                     /translation="MIGSERLLLSGNEAVALAMKSAGITLGTGYPGTPSTEILENFSA
                     RGGRAQWAPNEKVAFEVALGAAYGGARSVVTMKHVGVNVAADPLFTAAYTGVPAALIL
                     ISADDPGMASSQNEQDNRHYAVAAGLPMIEPSDSQEAHDFFLHAVAVSERWHIPVLFR
                     MTTRVCHSKTIVNTGLRQELAAPAPPAYVRSIMERVMIPAYARPAHRKLRQKLAEIAK
                     SNETSPLNTRTSGVRTLGIISSGIAAMHAREVAPDASFLKLGTTYPLPMRIIRDFAES
                     VDRCVVVEEGDPYLADSIRAAGVPVHAKPDMYRFGELNVGRVKRILANDTSPEEPVPS
                     GKPPQLCKGCPHRVVFEAFRDLGCIVAGDIGCYTLGVLPPFEAMDTCVSMGASITVGL
                     GLRHVLPREQAVKVASVIGDSTFAHSGLSGLVEMVYNPPATGHVVVIVDNGTTAMTGQ
                     QEHPGTGRTLDHEPTGKLVFEDIARAMGIKEVHVIDPLKEEGEFARLLAETLAKEQPA
                     VLITRRACLLAAARIRAYEQRALQGKEACNADGK"
     gene            22323..22970
                     /gene="lolA"
                     /locus_tag="PLT86_03270"
     CDS             22323..22970
                     /gene="lolA"
                     /locus_tag="PLT86_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOT35668.1"
                     /translation="MVHVRRWVLMAAGVLGVSVAAGAITGNEVLAKIRGTYDKVNSFQ
                     AAFEQTFEWKLAGTTQTMRGTFSMKKPNKFRIQTNVQTVVSNGRVVWSYSPATGQVII
                     TNYDPATMPLRPDNFLFVFPDERRTTYVRSERLSGEEHHVVDVTPRDSTLGISKMRVW
                     VDGRSWLAKKVQYTSVSGDVTTYLMNAIQTNTSIPDSVFTFTPPPGVDVIDFRNR"
     gene            22981..>24322
                     /locus_tag="PLT86_03275"
     CDS             22981..>24322
                     /locus_tag="PLT86_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT35669.1"
                     /translation="MSLRRFWQVLVVAVVAAESVFSATTPEREYPRAWWVWFVDKGPE
                     QRADDDSAPVFPAYLARVHGIAPVRTTTRWFNGASVLASEEQLAAIMELPFVRGVAPV
                     LPLRGERPLPADGLLPAERPTRSGALDYGLSLTQNRMIGADSLHALGLNGQGVLIAVL
                     DEGFPYLTHPAFQNLQIAARRDFIDGDTLIVGYSQHGSQVLSCLGAYQPGALIGPAYG
                     ARFALARTEFGPTETRSEEDYWLRAAEWADSLGARVISSSVGYNYFDNPDSNYALADL
                     DGRTSLITRAAQKAAGKGIVVVNCAGNERGTPSYWNGKLLMPADGDSVIAVGAVTLAG
                     TVTSFSSFGPTADGRIKPDIVALGSSVRMISESTGYTWASGTSYSTPLIAGVVAQLLQ
                     AHPAWDPIAVRTALRLSGSHALEPDTTRGWGLANAVKALAADSAVFGRVNGDLSG"
BASE COUNT         4901 a         7138 c         7076 g         5207 t
ORIGIN      
        1 tacggcgccg cgggattgct ccaacttcaa cagcacggct tgctggatga gttgcagatc
       61 gtggtcagcc tggtcactat caccctgttc gtgccgtgtg tggccaacgt gttcatgatt
      121 gccaaggaac gaggcattcg aacggcgatc tggatggtgg tgttcatctt cccgttcgcg
      181 tttctggtcg gcggcctggt tcggtggggt atgatcctta ccggttccgg gtgagggcgc
      241 tgaaccttct gcgtgttctg ggtgtttgat tttgtggggg cgtgacggag tgcgagggaa
      301 caatgatcgt ctgtcctttg tgcagtaagg aaatcccaca ggatgtggag cagtgcagga
      361 cccgctgccc catgggaaag aattgcggcc tgatccggtg tccttattgc gggtacgaaa
      421 tgcttccaga atcgcgcctc gtcaagcttg tcaggaaatg ggtaggcacg aaatgaaaca
      481 cctgccacga gaatgcgaga acatcctggt gcaggtctgg tcagcccacg agaaaggcga
      541 caaatctctg ggtaggctgt tccttgccca tcctcacgcg gtcccggtcg tcgatgaact
      601 cctcagagca gggctgctgg aacggctcga cggcgagata tggctgacgg aacgcggagc
      661 gaaagccgca cgcgaacttg tgcgccgcaa ccgccttgcg gaggtgttgc tccagcaaat
      721 ccttgaagtg caacccgaac gagttgccgt gaatgcgtgc gcgtgggagc atgtgctttc
      781 ggcggaagtc accgacacga tatgcacgtt cctggggcac ccgcggcact gcccccatgg
      841 gaataccatc cccccggggc cttgctgcgc gaagagttct cgaaccgtga cacccctggt
      901 tcaacccctg acaacgctcc ccgtgggagg caccgcgaga atcacgttca tcgcgcccag
      961 agtcagcaga aggcttgata gactcgcagc gttgggcgta gtgccgggaa caaaactggt
     1021 tctgaaacag aagtctcccg cgtacgttat tcgggcggat cagacggata tcgccattga
     1081 agaagacgta gcgaaagata tcttcgtgcg agaaataaaa gcggaggaag aagggggatg
     1141 atgaccgggg aatgcgcaca ctcctagagc atccccgtgg ccgcgtcgat ggccgacacc
     1201 cagtccgcag gagtaacacc ggggctgtcc acctgattga tgatgtaata gttctccaga
     1261 acagtcatga gttcgtccca ttcggccggc acgccgtgta acgccgtttc gagatcgctc
     1321 agacagtccc ggtagaacac gaaatccccg gagaatgcaa cggtctcgat tacctccccg
     1381 cggaggagaa gactcaagcc gatagtccgg cctccggccg tggtgaactg ggcctctcgc
     1441 aggtccagcc ggggatctct tcgcgcgcgt tccctgagtt ccgccctttg aggctttctt
     1501 cggggctcgc tttcgctgcc cgcaagaact ttctgattga tggcggcctc ttcggcggga
     1561 gaaagacgtt cctccctgaa ttccgtgcgg cactgctcgc ccagcttcgc gacaaatacg
     1621 tcaagaactt ccgccggtcc aactgcaccc ttcgatattc cggagagcga gacttccacg
     1681 gcgttgcgac cgcccggttc ttcgcttgta cacgggagag ttccggtgag aaccagtgcg
     1741 ttcccaacac accctgcata cactgccgaa aaaagaattc ggtggtacct cactgaatca
     1801 ggtggggtga agtgtgcatc cacgccatac gatcgacacg tcgccacgag agaaggaacc
     1861 atgcctctgc acgcgcgggt gatggagttt tccgcgtcgt cctgcgggat aatcatcgag
     1921 aacagcagcg tcccgggaac tacgctcatc tcgtgcccgc ctaccgaacg gcgaagcatc
     1981 ggccatgggt atcgagttgc cgcctcctcc agatcgattt cggcgtcctg atacaggccg
     2041 atactgagaa ctcggtcact cgggaacgcc gccagaaacg tgcgacgccc gtgggacgcg
     2101 gtcaactcgg caagggaatc agttgcggcg aagaaacgcc gcgcgggaac gaccccgagg
     2161 ttcaccaatc ggctgacatc aatgttcttc atgcagcgcc cctgctgaaa aatcgccccg
     2221 cgagatagcg ctttgcgcct ctgtgcaacc ttggacgcac acacgaacgc ggcgacacaa
     2281 ctaccgaacc ctgcgcccct cagttgaggg aagtgccgtc cggcaggtgc gagggacgcg
     2341 gcgccagtgt gccgtcgtcc agcctgatgt gggtctggaa cccgcggaca ccctcacgta
     2401 gcgtgattac ccgcgcgccc ttccggaatc cttccttacc gtaacagttg taccccgaac
     2461 cgcggccgta cgcaagtgtt acgccctcga gtacgccctc gtaatcgttg acatgatcgt
     2521 gccccacgaa acacccggcc acgcggctcc tttccttgaa ggcatcgaaa aggccctcat
     2581 ccatcccctg catgcacacc ttttcgttga gaaaacctga gcacggtgtt gtctgccaga
     2641 cggtcaggtg ttgcaccagt ggcatgtgca cgaacgccaa tcctgtgatg gagtccccgt
     2701 cctgtttcat gaggcgatca gcttccgcgg agaaccacgc gatctgatcc cttttcaccc
     2761 agtcccaccc ggatgcaact ccttctgcgc cctgccccga atcgaggctc catacaatcg
     2821 cgccagggcg cgaggttcct cttttgtaga tcggaacggc gtaattgccg tgcccgaaga
     2881 tgctttcggg gccggactcg taaaggctca gtctgctgcc ggccagtgtg tcctcgatcg
     2941 cttcgtaggt gaattcctgc gcgtcgtggt ttccctgaac gaaagcccat ggcacgttct
     3001 tcgcatcgaa gtacctgacc acgtcagccc agtgaatgag tgggtcttta caccacgccg
     3061 gatgaacaat atcgcccgaa agaacgataa ggtccggttt ttctatctgg agaacccggt
     3121 cgaaaagttc gaacgtttcc ctgttatggg gtaagtcagc aaagtgcgtg tccgtgaact
     3181 gaacgagcac aaaaagcccg tcgggacgga attccagtgg ttgcatacag tgccctgatt
     3241 cgcgggtttc cggttacctg gggcggatat gagcgtctga cgaaacgcgc caccggatgc
     3301 gctccgaaaa tggtctgctt cccgccctga gaagagggtg attgcttatt agacggctca
     3361 gtcgtcgcgt cggaaaatca ggtagagcac cagtgcgacc accgtgacga cgatgctcca
     3421 tttgactact tccttccacg ggaacgcagc gcacggaaca ttttcctcga aatctttgtc
     3481 cgccgccatc acgccttccg gttgctcttc ggcctcttgc tcggttgcag tcttcgtttg
     3541 ggcgtcttgt tcaaccgggg ccgtcgcctc agcctcagcc gattccgtgc cctgcgaggt
     3601 gatttcgatt tcttccggtg ggacaggatc gctcttcttg tttgccatcg gtctgctcct
     3661 tgcgtgaaat ggcttacttc tgagcacgaa tgacgcgccg tgcaacgtgc tcccgcctta
     3721 acaacctaac cccgccgaac ctgattggca agtgaacgac gtcacgccat ggcatcacgc
     3781 gcccgccgct gccgggtagc gcactcgcac tggaaattcg tgagcgacaa ggtcgtgcac
     3841 cgtcgcagcg atttccccgc cccgcacggt gatgacggca gccgtctccg cgggaacgcg
     3901 gctcctgctg attgctccgg ggttcaaaaa cagcgtcccg cgtactttct cgaggaaagg
     3961 cacgtgtgtg tgcccgaaca gcactacgtc ggcatccatg cgcatcgtga aaaactcaag
     4021 cgatctcccg cttgtcggaa caacgtggcg cacgtgaaat cggatccctt tgtggctcgt
     4081 gtcgagcact tctgggaacg ggagatcgaa tgggtccgta tttccgcgta tggcatggac
     4141 gggtgcgatc aactcaagct ctcgaattac ctgcgacttg ccgacatcac ccgcgtgaaa
     4201 gatgcaatct acaccctcga agagggtgaa cactgcccca tttaatcgcc cgtgtgtgtc
     4261 cgaaagaaca ccgacgcgga aaacttcatc attcagggca tctttcgaga ttgtcatgtt
     4321 cctaccccga actgccggtt tgttccccgg tgaaaccgcc ttcggtcatt gcccgcacat
     4381 ccagaagctg atcgagttcc gcctcactca gatctgttac ttcgcgcgcg acatcgcgca
     4441 ctttgcgccc ttcggccatc gcgcgtttcg cgatgtgcgc agcgaggtca tacccgatgc
     4501 gcggcgcgag ggcggtcacc aaaatcgggt tcatttccac aaggtcagtc attcgtctgg
     4561 catttacgcg gaaaccggtg agcgctttgt ccgcaaggag gcggcttacg ttggccagaa
     4621 gggaaatcga ctgcaacagg ttgtgagcaa tcagcggaag catcaggctc agttcgaaat
     4681 tccccgactg cccgccaatc gcaatggcaa gatcgtttcc catgacctgg gcgcatgcaa
     4741 gggtcgtcgc ttcacagatg accgggttca ctttcccggg catgattgat gacccaggtt
     4801 gaaccgcggg aagcacgatt tccccgagcc cggcctgagg cccggaattc atccaccgga
     4861 aatcgtttgc gattttcatg agtgacacgg cgacggttct gatctgcccg gagagctcaa
     4921 cagcggcgtc ctgagcagat tgggcttcaa agtgattgtc cgtctctctg aaccggatgc
     4981 cggtcaattc gctgagtttc tccgccacac ctcccgcaaa cgctttgtcc gcgtttattc
     5041 cggttcctac tgcggtcccg ccgatggcca gctccgcgag ccgcggcagg gtggcttcga
     5101 ttcgtttgac tcctttcgac acttgagtct ggtagccgcg aagctgctgg ccgaaacgga
     5161 ttggcatcgc gtccatcaga tgggtccgcc ccgttttcac cacatcgtcg ttttcttcgg
     5221 cgcggtgtga tagcgtatcc gcgaggtggt gaagggccgg caacaactcc tccaccactt
     5281 tcagatgggc tgaaacgtgc accgcagtcg gaattacgtc gttcgaagac tgggacatgt
     5341 ttacgtggtc attagggtga atgggtgtct tcgatccgat cactccaccc agcatctgaa
     5401 tggcacggtt cgcgataacc tcgtttgcgt tcatgtttgt ggaggtgcct gaacctgtct
     5461 ggtacacatc cacgggaaag tgctcgtcga gcttcccttc ggccacctca agtgccgctg
     5521 cacgtatgac gtccgcaatt cttgcatcca ggtagccgag ttgttcattc acgtcggcgg
     5581 cggcgtattt gatcaggccc agcgcgcgaa tgaaagatcg aggcaagcga atgccactta
     5641 cggggaagtt ctcgatcgcg cgttgcgtct gagccccgta gagtgccccg gccggcacct
     5701 tcacttcccc gagactgtct cgctcatatc tccaatccat atcgagcctc gtcagaacgc
     5761 gatatcaatc aggtaccacc tgccaggtgt catcgatgcc tttcaggccg acaatacggt
     5821 atttacccgc tgtgaaatca ccttcgatgg aagcgccgcc ggtgaatcgc gtgacccgtg
     5881 cagtgccttc ggtgttcagc aggtcgtgtg cttcaatggg cgcgaaccct gtttggcggt
     5941 gtacatctgc aaggattttc gcccggtgga aatgagcgga acgcgcggcc gattccaccg
     6001 gtgccccggg gcccgattgt actttcggtt ctggtgcggc tactatcggc gggcatccgt
     6061 acagcaaggc atacaggaac gcgtccttct cagaaactgc accagatttt ccaagcggcg
     6121 ccggcgttac cacacaatcg tgataggtct ggttgaagag cggtaccggg atgccgaacg
     6181 tgggggcatc gggagactcg tctgccatgg cgaccggcgc ccagtagcag aaatcgatgt
     6241 agggcacaga ccaatcagtg ggctcctccg aactgagcac gatgccccgg gaacgcaccc
     6301 actcgaagca ctccgcgcgt gcttgaagac tttgttcgcg cgtcatcggg tggtccgggt
     6361 ggtagcactc cagcatctca atgatagaga acacatcgag gtaggttgcg cgcagcggta
     6421 ctcgcctccg gaggatctcc tcgaaattcc tccgaacgaa tcccagcgca ttcttttcgc
     6481 aaagaaacgt ctgtgcgccg ccgtaccacc acgcttttcc gggcacactc ccatcttcag
     6541 caacgcgcgc gaggtattcg cggtaagtcg gtgcgtccac gtagtaatcg atatagttgt
     6601 cgtgcgtggt gaacaaccag cccagacggt caacagcttc ggcgagcttc ttgaacccat
     6661 cccacccgcc tgcttcttcg cacggtggga ggtaatcagg atggaggttg tcgtaccccc
     6721 gcgtccccca gccatcgagg tggtacacga gtctgtccgt gggatatatc tcacgaagcc
     6781 gctcaactcc cctgatgctt tcctcaaatg tgcgcaggtt atggttcttc ccggggtcat
     6841 cagggtgata gtagtgggaa tccggctgga tgtgatacaa cgtataaccg gttgcgacgg
     6901 tagtaccttg cagcaatttc acctcgggga cttccgtggc cttcgtctcg agggtcttca
     6961 gccgtccgat tgtccttgcg tattcccggt acttcgccac aagtgcgttg tggtccacgc
     7021 tgccgaaaag gtggtaccga acctgacgca gatatgacag actgcccatg ctcggatgcc
     7081 acttgggtcc gacttccgtt ggcccgccgg gggggtgaga gaaatgcaat ccgccgtcga
     7141 aaggggtctc cagtatgccc atgtaggcat cgccgggccg aattgccccc caccatggca
     7201 tgtaaatggc tcgcgtccag ttcaacccga tccaccacca atctattttg cccggccagt
     7261 cagttggcag aagcgcgccc tgcatcgagg gcagtacatg gtgaacaccg gggccccggc
     7321 cctcaagaac aaaggggcgc ggatagcgca cctcgcggac cactcgttga ggatcgtgcg
     7381 ccggaatcac cctcacgacg agttcatccg atacgccgtc tatcgccacg ataatcgcga
     7441 gagacacgtc cactgaacgg ttaccggcag gaaaccctcg caacacgacc ctgacgcctg
     7501 ataactcacc agcttcatag agtgtggctt gcttctggtc cgcggaagcc agcgcaaccg
     7561 gtgtttcgtg cccgttaagt gcgagcgtca ggtcgtgact ggtttcaacc atgcgccacg
     7621 ggcctccacc cgatcgctgc atggtcaacg tcattgtgac ggtgtcaatg tggacggtta
     7681 tgttgttcga tgttatctgc cagatggccc cattcgcggt tgttgacggc atggtatgtt
     7741 ctccggatca gaacggcaac cccgaggtcg gagtcgggtg gacaaggtgt cgagaaggta
     7801 ccgtgaaacg cgccgcacca caaccgcacg atcgccgacg ttggggcgcc accgttcccg
     7861 gcgtagaatt acctgtgtgg cgacgtgtca ctactttgga aggggagttg cagaatgacc
     7921 tctcgcgagc gtcttagagc gattttcgct cacgaaccct acgaccattt cggaatcttt
     7981 gagcacttct ggggcgaaac aattccggaa tggaggaagc aagggttccc ggaagacggg
     8041 gatccggcgc ggtttttcga ctacgacctg cggggtgtgg gcggcggccc gaacgcgaca
     8101 cccttccggg atttttcgga actcatccag gaaacggatg aatggaaggt cacgcgcagc
     8161 ggatggggtg cggcactgaa gcactggaag catcattccg gaacccctga gcacattgat
     8221 ttcgttatca aggacgccac aacgtggaga ccgtaccgcg atcaactggt gggtcctgct
     8281 gacccgacac ggcttgatat tgatggaacc cgcaaaaact ggaaagaagc ggcggcagag
     8341 gagaaatact gcaccttcag ttgtcttttc atcgtggaga cactgcgcca cgcgctcggt
     8401 gatgtccaca tgctggaaag ctttattctc gacaaaccct ggctgcacga cgtgaacaga
     8461 gtaattacgg acttctacat ccggcatttt gacctgcttt tcaaggaggt tggtgtgccg
     8521 gacgggatat tcatctacga ggacctgggg ttcacgaacg ggctgtttgc ttcaccaaaa
     8581 acccttgagg aactcatttt cccgtattac cgggagttcg tggacttctt caagggcaaa
     8641 ggcgcacagg ttctgctcca ttcatgtggc ggtgtcaccg aagcagttcc gatgattctg
     8701 gaagcagggt tcgactgcct ccagcccatg gaggccaagg ccggcgtaga tgttgtagcg
     8761 ctggcacgga aattcggcaa tcgaatcgcc tttatgggca acattgacgt gacaaaactg
     8821 aatacgaacg atcgcgcgat cgtgaaggcg gaggttcttg gcaaactcaa cgcgttatac
     8881 gacctgggcg caggttatgt gttccattct gaccattcga ttccacccga tgtaacattc
     8941 gacacgtacc ggtacgcggt tgacctgtac cgggatttct gcgccacaca cccttgcgga
     9001 gcatgaggta cgaaccatga acggagaacc cgaatactac gatatccggc agtcagcctt
     9061 tcagacctgg tttgaaaagg gactgcccta cgaggactac ctcgcccgca gcgaccccag
     9121 acatgcggaa cgctggcgga ccgctgagaa ggcgatcgcg ttgaccgcgg aacagcgcag
     9181 gctgttgggc tccttcgtcc gccagatgcc ggtactgtgc ctgtcgggcg tgtggtgtgg
     9241 cgattgtgtt cgtcaggggc cgattttgca cgcgtttgag aaggcagcgc cggttgtgaa
     9301 tgttcgcttc cttgaccggg atgagttccc ggatctcatg gacgagttgc gcatatgcgg
     9361 ggcaaaaaag attcctgtgg tcgtctttct gacggaggat ttctttgaag tccaacggta
     9421 cggggacagg acgttgtccg tgtatcgcag aaaagcggag cgcgagctcg gccccgcctg
     9481 cccggtaacc attggccctc aggatggaaa tgaggctgcc gtggagattc aggaatggct
     9541 tgacacattc gagcgagttc agctcatgtt gcggctctcg ccgccgttgc ggcgcagaca
     9601 cggtgattga accgcttggt ttgaaaaaga aggggggagg cgtttcgcgc tcccccgggt
     9661 aacgttgtcc tgttccgagg ttacaaccct ctcaatgctc gcccgacctg ctcaacagcc
     9721 tccacatgcc gctttctctc cgcttcgagg gtgggcttcc acggggtctg caggaatccc
     9781 ttcagacgct cgggcgcgat tattttccgg caatactcaa ccgtcatgcc gaagttgtca
     9841 ggatgcgacc agttgcttcc cgttggaacc tggtcatagc cgtgctcttc cagatcatgg
     9901 tatgccttta cgtagttcac cgtgtcgttg aacactacgt cgtaatacca gttgctctgc
     9961 aacacggatt tcggcatgcg tttgaggaac acttcccgat tgttccagat gtaatctgac
    10021 cagatccagg cgcgaacgcc cgccttctca acctgctcca cgtagaacag aaaatcgtgc
    10081 caccagagat cgaactggcg gatgacaacg tattcgaatc gccgctggtg gttcgcggtt
    10141 tcctcgtcca tgccgagatg gaagaacctt ggcgtatcaa acagcctgat cacctcttcg
    10201 atgaggtcgg cacataccgc gtagtacgtg tcggtagaaa ggcaacgtga ataagggccc
    10261 agccatacgt catggcaggc agaaaaattg agtttcggga tcggttccaa cccgagcccg
    10321 cggatgaact tcagttcctc ccggagtttc tgcgggctcc atgcgcctcg aacggcaatc
    10381 tccgggtggc tttcgtaccg aattccgtct ccgagatcga tgattaccat attcaggcca
    10441 ttttccacca tctttctgag aatgtcgcgc cagagcgact catcgaagcg gagatacggc
    10501 cgtgcggttg tgtagctgtc ggggcgttcc ggatcttcgc ggtcggccca catgttgtag
    10561 ctgaggtgga gaagatttgc tttgatcagt tctgatgcag ccattgctgc gctccttcaa
    10621 ctgaggtggt gggaaaacga aacggatcag gtgtcgaacg acaaggcgaa tggctcagaa
    10681 gcgcaggctg tgtgccggtg cccgacgtat gaaggctccg ttcccacgca ggcgggcggc
    10741 tgagtgtttc cagaagagcg gaaaactgca ggttggcccg tcgaagtgat tcttcctgga
    10801 acgccacgtg tctgttcagc cgggcaaatt cggcgcacgt tgcggtggtc gaatcgggat
    10861 ccgcgccaag aacgcgcgcc agcgaatcgc ggacagctct ggttgcagtg agcctccggc
    10921 gaccttccag gacgcgttgc gcgttctcac ggagggcgaa tcgcagctcc tcgaccgtca
    10981 gttcccggta cgtctttccg taacgctcga ggagatcccg ttcactgata ctccccgcgc
    11041 agtagccttg cagcaaagaa aacagaacgt cgccgccttc cattgaagcg ctttgcggag
    11101 ataccgccca ggcgttgtaa ctccagggat aaccggaggc cggcgcgaag ctcttcagat
    11161 cgtctggaac cgggtaatcc tctagagcgc tgatgaagct gacatagttg cgcagggtgt
    11221 ccgccgcctc cggactcgtc aaaaactgaa ggaaatcaac aacctgcgca agatgcccgc
    11281 gccgttcagc tactttgccg atggaaaaac tgagattgta gctgccatcg tttctcggtc
    11341 catccccgta cggggttgtt tctttggtca ccaacggcat tggaaagacg ccccagtcga
    11401 aatccacagc ctcggcgagt ccttcggtct cccaggatcc gtccatgtac atcgcaccga
    11461 tgccgttggc gaacagcatc ttggattgct gacgatccag cccattgtat ccccgtaccc
    11521 agtacctgga ccactctttc agcagatgaa gaacagcgag catctgcggg tcatcaaggc
    11581 gcgtcaggcc cttgtagatc gagatattgg attcgatccc gtccacctgt ccgtcgcgaa
    11641 tcaccgtatc gatttccggc acgcggtcct ggaacatcat gtcgctgagg aattccatgc
    11701 acgtgaaaat gtatttgtgc gggttgttgt ttgaatttgg catcaggtag ggcacgatcc
    11761 cggcgtccag cagcttctgc tgcacggcga caaactcttt ccaactggtt ggtggttgcg
    11821 tgatgccggc gcgggcgaac agctccttgt tgtagaagat ccgcacggtg aagaaattga
    11881 acggtaccca ccacaactcg ccgtaaatcg ggtcttccgc gttcttccat acaatcgggt
    11941 aaaacgtatc tttccaccgt ttccctggcg cgtaagggct ttcctcttga agctgttccg
    12001 tgaggggaac caacaacccc attcgagccc caaagcgaaa cgcgtcacct cctccgaaat
    12061 ccagaatgtc gggtcccctg ccgcttatga actgagtttc cgcccagacc tggtacccgt
    12121 caagcggctg gtagtgaagc cgaaccttcc acccggggtg cagctcctcg taacgccggg
    12181 ccaaagccgc gaacgcggga atccaaccgg ggtgttctcc catgtgtgtg gcgaagtaga
    12241 ttgttcttgt ctcgtctacg gtttgttcag gcgccgtttc gcgcggcgcg cacccgcaca
    12301 tcaccccgaa cagaagacag attgctccct gcacgacgag atttctgcag gttctgatgg
    12361 ctggaagcac gtggtacgct accattcggg tgccgtactc cttactttct tggaagggtt
    12421 ggtggcctgg tgccctgttg agcaaatgag ctcaatccct ccgaccgtgg aacctccact
    12481 ccgctaccaa tggtaattcg tatgagaggt ccacagaaac cgtcgtcctg tcgtgtgcta
    12541 ggagtcctgc atgcctgaag taaccaaaga aatggttgtc gaagccctga gcaaggtgaa
    12601 cgaccccgag ctccatcggg atctcgtctc gctcggaatg atccgcgacg tcgccgtgac
    12661 ggatggagcg gtcaagttca cgctggcgct cactacgatg gcctgtccgc tgaaaagcaa
    12721 aatgcaggag gactgcaaaa ctgcagttat gcgcatcccc ggcgtcaggg aagtcacgat
    12781 agaccttact gccgacacgc cacgcaatgc acgtccggcg tccgggaaaa aggtcggtga
    12841 tattgtgctc caggatatgt tacccactgt gaagaacgcg attgccgtgg cttccgggaa
    12901 gggcggcgtg ggcaaaagca cggtttcggt gaacatcgct gctgcattgg caatggaagg
    12961 cgctcgcgtc ggattgctgg atgcagacat ttatgggccg agcataccga tcatgatggg
    13021 agttacggaa caaccggaga tcaccgactc agaagagata attccgatca ggaaatacgg
    13081 gatcgagttg atgtccatcg gcttcgtgat tcctgaggaa caatcggtaa tctggcgcgg
    13141 cccgatggtg gcgaagatgt tgcagcagtt tctgagtgga gtgaagtggg gcgaacttga
    13201 ctatctgatt gttgacctgc ctccaggcac gggtgatgca cagctgacgc tcactcagtc
    13261 ggtacctttg gccggaggac tgattgtgtc gactccgcag gcggttgcgc tggcagatgt
    13321 gcgccgcggg attacgatgt tcaagaaggt tgatgtgccg attcttggcg tggtggagaa
    13381 catgagcacg tttgtgtgcc ccaagtgtgg ccacgaggag cacattttcg cgtatggcgg
    13441 tgcgcgaagt gaagccgaaa aactgggtgt tccgttcctg ggggaaattc cgattgacct
    13501 ttcaatacgc gaaggtgggg attccggcgt tccggtcgtg gtggcgcatc cggattcacc
    13561 cgtcgggctg gcgtttcgga agcttgcgcg cgacgctgcg gcggcggtga gcgtgagcaa
    13621 cctgaccgca cggaaagcgg aacgacatga gccggcaaga accggtaaag aattgccgat
    13681 gatgaagtga acgggggcaa aacgagagtg ttgttcgtcg cggaggccgg gctcgcggga
    13741 taagaggcga gcgggcccga cctcttctac ctcaagcgtc ccacgtgtga ggacgtatcc
    13801 ttcatgattt gatacattcg gaattcgccg gtatctagag tgtcaaatag agttacgaaa
    13861 tggccagaag ggatgagttt tttggttatt gagccagtga cgaggttctt tgccacgtat
    13921 ccccctccgc cgccgatgag ctccgcattg attctaacgg taacgaacgc ggaatcaccg
    13981 gcctccccgg ttctgattga ttcgaaaacg gttccctccc ggtaacggat gttctgattg
    14041 acaatcaaca ggtagtggtc acggccgttt ctcagcggcg tcacgagaac tgccgccttg
    14101 tcacgttctc cccatcccga tttgaaggga cgcattactg aatccggctc ccaagccgat
    14161 actccgtcaa caaagaacga tctggtgggg tatggaccgc cgacggcggg cggctctgac
    14221 atgacggggt ggaatatctc cgcgggaccg agataggggc ggatacgcgc gacgtctgcg
    14281 ttgacggcac gtatgtggcg gtaaatcgcg ccgaaccccg tcggccggta cgacatcgtc
    14341 tcagatgacc attcagaggt gaaaacaccg ggaaaccgag cgtatcggcg cgtgtgatga
    14401 cggtccgccc aatccgcgcc ccagtaccgc ccatcgtaca cccgcgttcg acctgtgggt
    14461 ctgcaattct cgtaatcggg caaaacgatc gcatcaatgt aatgggaata cgtgagatac
    14521 actattcctt tcgctcccca gcccaaccgc gtaaagacgt ccatccgaag gctcgcctcg
    14581 tcaatcacgc catagggata ccacacgcac ggcccaccca tgccaaactc atcggtgaca
    14641 tgggttaccg accaccaggg tgtgcccggc gcattgtccc ggaccgtgcg atagaatcgg
    14701 gcgaggctcg cgttcaacca gtccatcggg tagatctgag ggttttctgc ggtgtctgga
    14761 ttcatgaagc ggaatgcctc atggccgatt acggctggtc cgccggcgcc ggttgtgcca
    14821 atccccctca gtattctcgc gatcctgagg ctgtcgttcc atacgaacca accgaggaac
    14881 gtgagccagt cctttcccgc caatggtccg atttctgtga gctgtgctct gagcagtgaa
    14941 tcaagccgca acgccttgtc cacttcgcga tcgttttgtg gctcgtcgca gatgtaccac
    15001 ccgatcacgg aggaatcttc gggcgtggcc cagaccggat tgctcagcgc gcgccgtacc
    15061 cgcgcaacgt cgaggtgatc gctttcccgc tggccgtaca cgtcgggatg gaggggcagg
    15121 cagaaaagcc gcgcagcgtt tgcagcgcgt accgtcgcaa aacaatgccc ttcattctgg
    15181 agattgaccc cgagcggggt catgatcatg tcaattcccg cggcttttat gctgtcgtag
    15241 ctgtaataat tgaccttgag cgcgcggtag ttggcgacga accagtcagg agtgccggat
    15301 cctgtgaagc gtggaaacgg atcggcaaga tcgtcaggcg ccggcggata accccagact
    15361 ccgacgagaa ttgtggtccg gggatctgga acgcccgcgc gaacggcaag ggggagggag
    15421 gtattcagga gaaggcatga cgtgacgacg gacaacgctt tgaggtggcc ggacattggt
    15481 tctccacgtc ctcaaccggg aatcaggctt ctgtgccatt cgggcaggag cgcccacgcc
    15541 cgagccttga aaaaaccgat tttgaggaaa tccgacaacc tgcgctaaga cttgccgttg
    15601 ccttctgcgg cgtgcaaagt tcgctcgaaa ccggggagcg aacgttccat catctccttt
    15661 ggaacggtga gcgagagccg gaaatagccg ggtgtgccaa accctgaccc tggtactgcg
    15721 aggattccct ctttctgcag taatcgaatg aacgccacgt catcgggaag cggcgatttc
    15781 gggaacagat agaaagcccc tccgggctta acgatttcgt aacccattcg ggtcagggcg
    15841 tcccatagaa aatcacgttt ctcctgatac ggcgttgcgt caactcgcac atcgcctgcc
    15901 tcggccacca cgagctgcca gatcgccgac gcattcacga accccagaac gcggttggcg
    15961 aacgtacacg cgtcgaaaag atcggccgct tcgggaagca gcggcgaaat ggcgatgtaa
    16021 ccgatgcgtt cgccggggat tgccagcgct ttcgaccagg aatacgttac gacggagcgg
    16081 gagatgagag atgcggtctc cggcggttcg acaccgtcga atgtgagggc gacatagggc
    16141 tcatcgctga cgattgtgat cgggtgattc gcctcctcga tcaactcgtt caagcttctg
    16201 aggaccccgg ggccgtacac tgctccggtc gggttgttcg gggtgtttat gaggatcgcc
    16261 ttcgtccgcg atgtaatcgc cgcgcggatt cttcgcgtat caggcatgaa cgcatcgtct
    16321 gtaggaacaa ccaccatcac cccgccatgg ttctcgatgt agaaccggta ctcggcgaag
    16381 tacggcgcca ggacgattac ttcatcacca ggatcgagaa gcgacttaag cactacgttg
    16441 atagcgcccg cagcgcccgc agtcatcaat atgtggtctg cggtatacgc aaggccggtt
    16501 cgccgcgcca attttctagc gataacggcg cgtacctcgg gaaatccggc gttgggcata
    16561 tacgcgtggc tgcgtgggcg gtttcgcgcg acgatgcgct ccagaaccgc gataacctcc
    16621 ggcggaggtt caatgtcggg gttgccgagc gtgaaatcgt acacgtgttc cgggccgcgt
    16681 tccgccttca aacggatgcc ttcctcaaac atccggcgga tccaggatga ttgttccagg
    16741 tttgtcgcga ttgtcttggc gattcccatg tacaccttca ggtcagatga ggcctttttc
    16801 cttgcgatga tctaccacgc gcttcgcttt cccgatgaac cgttcgagag tctgaggcgc
    16861 cacaagctcc acgttcgccc ggattccggc gacgatatgg atctgacgtt caatgtcttc
    16921 ccgcaacaca tgcatctgac tcatcttgtc cgaaaaacgc tccggacgca gctcgacccg
    16981 gacggttacc tcgtccagtg ttcccgggcg gtcgacttcg atcaggtagt gcggtgccgt
    17041 tccttcgacg cgaagaagcg cctcttcgat ctgcgatggg aagacgtttg ctccccggat
    17101 gatcagcatg tcgtcactgc gcccgataac tcttcccatc cggatgccag ttctcccgca
    17161 cgggcacggc gtcgggtcca gtcgagcgat gtcgcgtgtt cggtaccgca gaatcggcat
    17221 cgcctgtttg gtgagcgcgg taagaacaag ctcgcccggt tcgccgtagg ggactggctc
    17281 cagggtatcc ggatctatgc attccacgag gaaatggtcc tcctggatat gcattccgtt
    17341 gcgcacaacg cactccccgc tgacgccagg gccgatgacc tcgctgaggc cgtagttgtt
    17401 gaaggcaagc aaacccattt gcctttcaat ctccagccgc atgtcctcag tccagggctc
    17461 accaccgaaa tgcgcgaact tgagggggat cttgtccggt gtgtatcctt gtgcgcgcat
    17521 catttctgcg atgttgagcg cgtaacttgg cgtgcacacg aggacatccg cctgcaagtc
    17581 gttgatcaga acgatctggc gttcggtgtt tcccgctgcc gcgggtacta tcgcggctcc
    17641 aaccagctcc gccccgtaat gcagcccgaa gccaccggtg aacagcccgt atccgaaggc
    17701 aatgtgaacc atgtggtccg gcctgagccc gccggcgacc agaaatcgcg cgcagaggtt
    17761 cgcccagagc gcaaggtcat cccgggtgta cgcgacgaac gttggttttc cggttgttcc
    17821 tgtggaacca tggatgcgca cgatttcgct gcggggagtt gcaaggaacc cgagcgggta
    17881 gtgttcgcgg aggtctgcct tcgttgtcag aggcaggcgt ttcaaatcgt cgagagaccg
    17941 tattctgtca gcggtcacgt tgccttcagc gaaggccttc ctgtagaacg ggacgtttgc
    18001 caggcgggca acgcagtcac gaagtcgatg tacctggagc gcttgaagct cgttccttgg
    18061 aagtgtttcc gccggatccc agatccggtt ttcaaaaagg aattctgcca tctcgtcggt
    18121 tttttaccgg agctgccttc gcaggcttgc cggcgtctcc ttcccgtcag cggttgtaca
    18181 cttcgacgct accgacgacg ttgatgccgg ctgaattgag gagcgcgatc gcggcgtcag
    18241 ggtcgtcgaa tcggaaaatc agaaccgcct tgtcgcctct gccaaacgta aaagcgtaca
    18301 tgtactcgat gttgactgcg ctcttttcga acactttgag gacttccagc agcccgcccg
    18361 ggcgatccgg tacctcaatc gccaccactt ctgtgtcctt gaccacgtac cccttgtctc
    18421 tcagcagctc atatcctcgt ttccagtctg aaactatcaa ccgcagaata ccgaactgct
    18481 gggtatcggc aagagagagc gttctgatat cgatccctgc atctgcaagc tcgcggcacg
    18541 gggcggtcaa ttgtccgggc ttgttctcga ggaacagcga cagttgatgg attttcatgg
    18601 tgcccccttt ctcgtgtacc gtgcgatttc gatcatccga gattccgttt gtcgatcacg
    18661 cgtttcgctt tgccttcgct tcgctggatt gcatgcggtt caacgaggcg tacctccgct
    18721 ctgatcccga gcgtatgttc caactccgat tccagtttcg tgtgaagggc ttccagtgcg
    18781 ccgatacggt cactgaacac ctcggcggtg acttccacct gaacctccat ctggtccagc
    18841 cctttttcgc gcgtcagaac gatctggtaa tgtggaagcg tgccttccac ggcgagaaga
    18901 gccgactcga tctgggacgg gaagacattc acgccacgaa taatgaacat gtcgtctgca
    18961 cggcgcccga ttcgtttgat tcgacgcagt gtgcgtccgc acgcgcacgg gccctcgaca
    19021 aacgaggtaa tatcccgcgt gcggtaccgg atcatcggca tggcctgttt gctgagggtc
    19081 gtaaggacga gctcgccctc ttctccttca gcgcacggcg cgttagtgac cgggtcaatg
    19141 atttcggggt agaagtgatc ctcgaagatg tgcaatccgc actgacagga acactcgatc
    19201 ccgactccag gtccgacgat ttctgagagg ccgtagatgt cgtacgcctt gatgccgctc
    19261 atttcctcta tgcgccgccg catcgtttcg ctccacggtt ccgcgccgaa gatcccgaca
    19321 cgaacacgga gatcgtggaa atccacaccg agttcggatg cgcgttcgag caggtgaagg
    19381 aaatagcttg gcgtacaggc gattgccgtg accccgaaat ccttcattac catgatctgc
    19441 cggtctgtgt ttccgcctga aatcgggatc accgtcgcgc cgagcgcttc gcctccgtag
    19501 tgcgcaccga gtccgccggt gaaaagcccg tatccgtacg cgttctggat gatatccccg
    19561 ctgccgactc cgcacgctgc aaacgcacgc atcatcgcgc ttgtccagac ttcgacgtcg
    19621 tcctttgtgt acgcgactac tatcggtttg cctgtggtcc cgctggaggc gtgaagccgt
    19681 acgatgtcgc ccatggggct ggcgaacagc ccgaacgggt aggtgtcgcg caggtcattt
    19741 ttgaccgtaa acggcagttt gctgatgtcc tgaagggatt tgatgctgtc cgggttgaga
    19801 tcccgtgcgt ccatctgttt ccggaagtgg cctacattct catacgcccg ccggacgatc
    19861 tgctgaagcc gtgcaagctg caggtcacgc aggagcggca caggcaggta atcaggagcg
    19921 ctgacaggat tgaacccgcc caaaccgtgg ccagccgtct tcgtcatgat gatctccccc
    19981 gatgtgaacg cgcgtttact gctgcgtctt tgtacatgtt cgcccgaatt gaaaggccgt
    20041 tatgttcata tcgtgcagtt tctccgggaa tcctgagcga atcgccgcgt tccagagttc
    20101 ctccgggaac tcgaggtgcg cgctcaatgc tccgagaaga gcgacgttga tgcaccgcgg
    20161 actcgccagg cgagacacgt ccaccagctc cgccgacagc gtgacgccac cctctttcag
    20221 cacgtgacgg ttgacttcca gttgatccga cgagagtacc acaaggtaat ccgcgcaccc
    20281 gaacggaacc atcgggctcc acactttctt tccgaaacga acgtcgctgg aaacagaacc
    20341 acccctctgg ctcattccgt ggagttcgct cttcttcaca tcatacccgg cgcgaaacgc
    20401 gacatccgcg aggatatcag atgctttgat cacgccttgt ccgccgaggc cggcgatcac
    20461 cacgttcaca acgtcacttc ccgtcggcat tgcacgcctc ctttccctgc agagcgcgct
    20521 gttcgtacgc gcggattctg gctgccgcaa gcaggcacgc cctgcgggtg atcagaacgg
    20581 ccggctgctc cttggcaagc gtttccgcca gaagccgcgc gaactccccc tcttccttca
    20641 acgggtcaat gacatgaacc tccttgattc ccattgcacg ggcaatgtcc tcaaatacca
    20701 gtttcccggt cggctcgtgg tcgagtgttc gtcctgttcc cgggtgctcc tgttggccgg
    20761 tcatcgctgt tgtgccgttg tccacgatca cgaccacgtg tcctgtcgcc ggcgggttgt
    20821 agaccatttc caccaacccg ctcagcccgc tgtgagcaaa cgtgctgtcc ccgatgacac
    20881 ttgccacttt caccgcctgt tcacgcggga gaacgtgacg cagaccaagt cccaccgtaa
    20941 tgctggcgcc catggaaacg cacgtatcca ttgcctcgaa cggcggaaga acaccaagcg
    21001 tgtagcaccc gatatctccg gccacaatgc agcccaggtc ccgaaacgcc tcgaaaacca
    21061 cccgatgggg acagcccttg cacagttgcg gtggtttccc ggagggaacg ggttcttcgg
    21121 ggctggtgtc gttggcaagg atgcgtttga cacgcccgac gttgagttcg ccaaaccggt
    21181 acatgtccgg tttcgcgtgg acaggcaccc cggcagccct tatggaatcg gcgaggtaag
    21241 gatcaccctc ttccaccaca acacacctgt ccacagactc cgcaaaatcc ctgatgattc
    21301 tcatgggaag cggataggtg gttccgagtt tcaggaatga cgcatccggt gccacttctc
    21361 tggcgtgcat cgccgcgatt cccgacgaaa tgataccgag cgttcgaacg ccggacgttc
    21421 gcgtgttcag tggcgaagtt tcgttcgatt tcgcgatctc cgcaagcttc tgcctgagtt
    21481 tccggtgagc gggccgcgcg tacgccggta tcatgacacg ctccatgata ctgcggacgt
    21541 acgcgggcgg cgccggggcc gccagttcct gcctcaatcc ggtgttgacg atcgttttcg
    21601 agtggcacac gcgcgttgtc atgcgaaaca gcacggggat gtgccatcgc tccgaaacag
    21661 ccacggcgtg gagaaagaaa tcgtgtgctt cctgcgagtc cgacggttcg atcatcggta
    21721 acccggcagc gacggcgtag tggcggttgt cctgctcatt ctgactggac gccatgccgg
    21781 ggtcgtccgc ggatatcaga atgagcgcgg cggggacccc ggtgtatgcc gcggtgaaca
    21841 gagggtctgc cgcaacattt acccccacgt gtttcatcgt taccaccgaa cgagcgcctc
    21901 cgtaggccgc tcccagggca acctcgaagg ccactttctc gttcggtgcc cactgcgcac
    21961 ggccaccgcg ggccgagaag ttctccagaa tctccgtaga cggtgtaccc gggtaaccgg
    22021 tcccgagagt aattccggcg gacttcattg ccagtgccac tgcttcgttt ccacttaaca
    22081 acagacgttc ggaaccaatc atgcctgccc cgcgaatagg tgaagcgcgg cgtcacgcct
    22141 cctcgaaacc ggtcggatat gcgctctgac gcccacacac gtgtttcaga gaatacgttt
    22201 gcgccgccaa acggtgcaac actccgatgg gtgggtcttc tttgcggggc acgccgtttg
    22261 cattgacatt ctgagagcgc ggtggcactt ttgggtacac gacaattgcg aaaggttccg
    22321 atatggttca cgtacggcgt tgggtactga tggcggccgg tgtcctcggt gtgtctgttg
    22381 ccgccggagc gattacgggc aacgaggttc tggcaaaaat ccgcggcacc tatgacaaag
    22441 tcaattcctt ccaggcggca tttgagcaga cctttgagtg gaagcttgcc ggcaccacgc
    22501 agactatgcg cggcaccttc tccatgaaaa agccgaacaa gttccgcatc cagacaaacg
    22561 ttcagacggt ggtcagcaac gggagagtgg tgtggagcta ttccccggcg accggtcagg
    22621 tcattatcac gaattacgac ccggcgacca tgccgctccg tccggacaac tttctgtttg
    22681 tgttcccgga cgaacggcgc accacgtacg tccgctccga gcggctgagc ggcgaggaac
    22741 atcacgttgt ggacgtgacg ccccgtgact ctactctggg catcagcaaa atgcgcgtgt
    22801 gggttgatgg tcggagctgg ctggcaaaaa aggtgcagta cacgagcgtc agcggagacg
    22861 tcacgacgta cctgatgaac gcaattcaga ccaacacctc gattcccgat tccgtgttca
    22921 cattcacgcc accgcccggt gttgacgtga tcgacttccg aaaccggtga cggtccctac
    22981 atgtctctgc ggcgcttctg gcaggttctt gtggtcgcag ttgtcgccgc ggaatcggtg
    23041 ttttctgcga cgactccgga acgcgaatac ccacgagcct ggtgggtctg gttcgtggac
    23101 aaaggtcctg aacaacgcgc cgacgacgac agtgcgccgg tcttccccgc ctaccttgcc
    23161 agagtgcacg gaatagcgcc cgttcgcaca acaacgcgct ggttcaacgg cgcaagtgtc
    23221 ctggcgtctg aggagcaact tgccgcgatc atggagctgc cattcgtgcg tggggtcgcc
    23281 ccggttctcc ccttgcgcgg cgaaaggccg ttgcccgctg atgggctcct ccccgcggag
    23341 cggcccaccc gcagcggcgc gctggattac ggactttcgt tgacgcagaa ccggatgata
    23401 ggcgcggatt cgcttcatgc acttggattg aacgggcagg gagtcctcat tgcggttctt
    23461 gatgaggggt tcccgtatct cacccatcct gccttccaaa acctgcagat cgctgcccga
    23521 agggatttca tcgatgggga cactctgatc gtggggtaca gccagcacgg ctcgcaggtg
    23581 ctgtcatgtc tgggggcgta tcaaccgggc gctttgatag gcccggcgta tggcgcacgg
    23641 ttcgcgctgg cgcggacgga attcggcccc accgagactc gttccgagga agactactgg
    23701 ctcagggccg cggaatgggc ggattccctc ggcgcacgcg tgatttccag cagcgtgggg
    23761 tacaattact tcgacaaccc ggattccaat tacgcgcttg cggacctgga cggacggacc
    23821 tcgctcatca cgcgcgccgc ccagaaagcc gccggcaaag gcatcgtcgt cgtgaactgc
    23881 gccggcaacg agcgagggac ccccagttac tggaacggca agctgctcat gccggccgac
    23941 ggagattcgg tgatagccgt cggcgcggtg acattagcag ggacggtaac gagtttcagt
    24001 tcgttcggcc ccaccgcgga tggccgtatc aagccggata tcgtcgccct gggttccagc
    24061 gtacgaatga tatctgaaag cacaggttac acgtgggcga gtggcaccag ttactccact
    24121 cctttgattg cgggtgtggt ggcgcaattg cttcaggctc accccgcgtg ggacccgatt
    24181 gccgttcgga ccgcgctcag gctctccggc agccatgctc tggagccgga taccacgcgg
    24241 ggatggggtc tggccaacgc cgtgaaggct ctcgcggccg acagcgcggt gttcggaagg
    24301 gttaacgggg atctttccgg gg
//
LOCUS       DAOSOO010000025        24250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2086_length_24250_cov_5.716429, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000025 DAOSOO010000000
VERSION     DAOSOO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 24250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24250
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_24250_cov_5.716429"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLT86_03280"
     CDS             <1..379
                     /locus_tag="PLT86_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35670.1"
                     /translation="ASGKVIGCHIDGTLGHLFADLVEAGIDFPESVVPAPVGDLTLEQ
                     IRDASSPNTIIWGGIPSAMFAPPFERDWVLSFAKRVVAVLGEERGLVLAGADQVPPNG
                     DIDLVRRIGDLLEELGPPTQIGS"
     gene            complement(441..2288)
                     /locus_tag="PLT86_03285"
     CDS             complement(441..2288)
                     /locus_tag="PLT86_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35671.1"
                     /translation="MRNRILIAPLTGTSGIPSISFGCPHFCVAAIVAVLISCFLANPA
                     SAQGSSSGFNPDAELARLRSTRANLERVQRMFYLGELTANSGREDVAQNAYGDVLSTF
                     ERLSGNARSLALPYAAQSALSLAAMTHVAYRDSPLRLERYAADSAQRAALLVRTREAY
                     NRALEFHYARATFEALFFRAYALQEWNTGGLDYAAERQPGTELLEQTIRDLNLSRQLL
                     DQAIREHQQILHLADSLGFSGGTGDRDVSEWVSLAREQLAAIPQQRDSLVAREEALQT
                     IYIERRASRWLARAEPLLWEKVTDLARRDARIADPFFDFVLQSRMVNDVFRPYIFGPS
                     GFLQAHDQARKGAQTVRSEEWMAERFAWRRRQEWRDGEIAMRLSREGLRHLLRSPEMV
                     DSVVVMLRTAADSLPGEARIALSRAAPQPPQIRMPDLPDLGGVDPRLLSGEEHAAKLD
                     EYQRYVTRMDSVSRQIEAYRAAVRSFSETVAAYVGQPLPSAADRYLRVRRALRATETL
                     LLDTLSALVLEQTRSALERSRLAAGWAPPGEVSASMQQTIADHAQTTSQNLRLAAAIS
                     REQAARYRVEVSRLRQTPLRDDLAETAATLERFAGTLEQQAALFAMGTG"
     gene            complement(2299..3297)
                     /locus_tag="PLT86_03290"
     CDS             complement(2299..3297)
                     /locus_tag="PLT86_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35672.1"
                     /translation="MSVLRRRKWVAKSLLSSAAAIVLVMGCGHAPEVRVAPAPEGESG
                     ELASDVDFAHAYLDLGTRYYEAQRFADAIEQFRFALELNPYSDEAHALIGMSNYRLGK
                     RELAERSFRRALQLNSRNLLAQNGLALVSNDERERAAALESAITYNPDVPELRNNLCF
                     MYVQSGDVERAIQECEASVRLDSANAYSRYNLGYAYQRQGRFEEALEQYRRALEIRAE
                     WARVLNNIGLVHYYRSELSNAVEYYRRAIAAEGSEAIYHYNLALAYEAIGVRLASAEL
                     RGEPARNVYGVAADTDWREMLREAASEYSTYLELAPGVSDAQRIRAKVAELRARAG"
     gene            complement(3453..3540)
                     /locus_tag="PLT86_03295"
     tRNA            complement(3453..3540)
                     /locus_tag="PLT86_03295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3503..3505),aa:Leu,seq:gag)
     gene            complement(3969..4724)
                     /gene="tpiA"
                     /locus_tag="PLT86_03300"
     CDS             complement(3969..4724)
                     /gene="tpiA"
                     /locus_tag="PLT86_03300"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT35673.1"
                     /translation="MRRRIVAGNWKMNKTAAEAAALVGELRPLVSDVSNVDVVVCPPF
                     TALAAVAPALVGTNIELGAQNMHWEKNGAYTGEVSAEMLLTLGCRYVILGHSERRQYF
                     HETNSEINKKVKAALAAGLTPILCVGETLEEREDGQTETIVKEHVTGGLLGLSPDQVG
                     KVVIAYEPVWAIGTGKTATPDQAQAVHAFIRNVLKQISNSAIADTVRIQYGGSMKPEN
                     AKELMGKPDIDGGLIGGAALKADSFAAIVKAAV"
     gene            complement(4960..6147)
                     /locus_tag="PLT86_03305"
     CDS             complement(4960..6147)
                     /locus_tag="PLT86_03305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT35674.1"
                     /translation="MAALTLRDIDVRGKRVLVREDYNVPLDENQNITDETRIQATLET
                     VRWIIDRGGKLILMAHLGRPKGKPDPKQSLAPVAKRLAEILGKPVVLAPDCVGEETEA
                     VVGRMKDGDVVLLENLRFHKEEEANDPEFAAKLAKLGDVYVNDAFGTAHRAHASTVGV
                     TAHFKQNAAGFLMEKELKYLGEALEKPARPFVAIMGGSKISGKIDVINNLLPKVDALL
                     IGGGMAYTFYKAMGLEIGKSLLEADKIDLARELLSKAGKKIVLPVDTVIADAFDNNAT
                     TKVAKRDEIPAGWEGVDIGPATVKEFSDIIKSAKTIVWNGPLGVFEMPNFAKGTAAIA
                     HAVAEATDKGSISVIGGGDSAAAITQAGLAERMSHISTGGGASLEYLEGKVLPGVAAL
                     TQK"
     gene            complement(6251..7249)
                     /gene="gap"
                     /locus_tag="PLT86_03310"
     CDS             complement(6251..7249)
                     /gene="gap"
                     /locus_tag="PLT86_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT35675.1"
                     /translation="MAIKLGINGFGRIGRLVFRAAVAQGGVDTLLINDLTSAATLAHL
                     LKYDSIHGRFPGDVSSEGENIVVNGKKIPVSKEKDPSKLPWKALGVDLVLESTGRFTD
                     AEQLKMHLDAGAPRVILSAPAKGKLDGTIVVGVNDKTLDKSWKLLSNASCTTNSLAPV
                     AKILNDTFGIVSGMMTTVHAYTNDQVILDFNHSDLRRARAGAMNIIPTTTGAARAIGE
                     VIPELKGKLDGQALRVPVPDGSMTDLTVVVGKATTKEEVNAVVKAAAEGPMKGILEYT
                     EDPIVSSDIVGNPHSSIFDAKCTMVLGGTLVKVCMWYDNEWGFSNRVVDLAKMWVS"
     gene            complement(7316..8356)
                     /locus_tag="PLT86_03315"
     CDS             complement(7316..8356)
                     /locus_tag="PLT86_03315"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT35676.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAVTVSAIDSGYEVLGFLRGWAGM
                     LEKDYEILTKERVYDIHRQGGTILYSSRTNVAKVKDGFEIVKRNLADLNVYCLVAAGG
                     EDTLGVAKKLADAGGNVVGVPKTIDNDVNATDYTFGFDTAINRVMEALDRLHTTTEAH
                     QRIMVVEIMGRHAGWMALHGGMAGGAHYIAIPEVPFDTDEIEKLCRERYAAGKKYAMV
                     AIAEGAEDPKLTAKLQRSAEKDSFGHIRLGSGRGIAEVVAEELQERTGIETRHIQLGH
                     LQRGGDPTAFDRVLGTRLGVKVVDMINAGEFGKMASLRGGEMVAVPLADAVGELKTVP
                     TDRLDVAKKFFG"
     gene            complement(8728..11172)
                     /locus_tag="PLT86_03320"
     CDS             complement(8728..11172)
                     /locus_tag="PLT86_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOT35677.1"
                     /translation="MRTKVTALILLTIILVCSSHVRGQDDEATSLVNHAAVQLANGDS
                     DSAEVLYRTALRINPRFALAAVGLGRILVSKDSIDAAKRLFEQAESWDRNKGYKQFGE
                     GLILKKRGQRDRAKTRFQEAFRKNNRFADALLEVARIQASGFVDRFAAKRTYRRVLEV
                     APNHPSAAYELGRLQEDSGELDEAIISYEAQLRANPQHGETMIRLGYTLLDKQHYWHA
                     RQRLYDALLNTSGKEGELSLALAATYMGDRQFARAHEAYMQALQLLPEDERALYEDIS
                     LITSPQEAAYVQRVSGEQKAIFLQKFWLRRDPTPATAVNERMLEHFRRVWYAKRHFSR
                     GRQPWDDRGQVYIRYGEPDQRASSSNPNFSTNHNVDMVRERYMNAIYGTNPPESLQRG
                     VLPVFPLVDPVQMLQSGFESGQPTQSTSTTGQQYGDVGETGDTGSDEAAASAVTSTQQ
                     AREQVPTFSAMAQAQLIRWEEWTYTGVLNGLNLTFVDRIGRGDFRFATPPPTTDMNKA
                     TVMQQYAPQEQLTIAAMEVPEQFEYDAQQAPFNFYYYVAQFREPTTSKTEVDIYYGLP
                     MADLQFRAQDDGTFKATVQNGFAVFDTLWNVQGRFLDRTDLISPTRPSQDRGSIHVDT
                     RSMVLDGGQRVLLSVQAEDVGSGRLQAYRENVPIAKFDSSTLAMSDIVVAGSIRPADS
                     TSARKFVRNGLHILPMASRSFRRGQPIHVYFEIYNLTRGTDYGDTEYEVEHAFRTGAG
                     EGGSILGSIGRILGGSSRRVGVARVMEGIRGSEYQHFQLSTSGIPKGEYTLIITVRDL
                     KSNQRVTKERLVMIGD"
     gene            complement(11233..12021)
                     /locus_tag="PLT86_03325"
     CDS             complement(11233..12021)
                     /locus_tag="PLT86_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35678.1"
                     /translation="MRSTRLCIRALLFLPRVRPRKNAATYSKRRLYAVAPSVATLAFV
                     IQCVDGCENRPFASRSPLPLYGDFRAYSIVDERRTASSPLDTISVYLFGDGVSDNAAV
                     TVNGTRVPRVLTAGNQALFGNSIPLQPAEILQVDVSTTWRSVRNVIGLPASRPVMVSP
                     AAGSYLNRDEPIVVRWSGITGGGVGLQLHRLSANGALGDTLWTTTAAAEDDSVVVPRN
                     VLSAVNDTTLLLSLWHESVGQGEGFTGVFYMSAGFGIRRLVRFN"
     gene            12156..13613
                     /gene="dacB"
                     /locus_tag="PLT86_03330"
     CDS             12156..13613
                     /gene="dacB"
                     /locus_tag="PLT86_03330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOT35679.1"
                     /translation="MRKGSKFFLAGCISIGLFATCIVDAFATETPWRCIAASLDSIVQ
                     RRALAPAQVGIAVWSFDRREWVYEKNAGRLFVPASNAKVLIGAAALHTLGPAKTFATT
                     LTADRVPISGDSVLTGNIYLYGGGNPDLVTGDLTGFAETLRASGVRRIRGAVVVDASH
                     FDSVNYALGWSWEDRPYPYCPPISAFLLNGNVVQVTVRPGSAPGRPVHVTLVPKDAGI
                     PVSVAARTGSEHNTSSRIRVERSNSGIVVKGTMGGRAEPITVWRTVPDPVRYAGSVFM
                     GALQDAGITVERGMSIGTSPRNLPVLARVYSDPLDVILRRYLKRSDNLLGETLVKQMG
                     VATSGRGTWEDGLSAVRRALRDIAGLDSNAYRLADGSGLSRLNEVSPRMLVQVITSAS
                     SSFSLAPEFLSALSTAGVDGTLSHRMTEDETRAYLRGKTGTLSGVSSLSGVVESRSGA
                     RFACAIMMNGTSPSAQTMRQAQDDVVRVLRNCIEQ"
     gene            13895..19942
                     /locus_tag="PLT86_03335"
     CDS             13895..19942
                     /locus_tag="PLT86_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HOT35680.1"
                     /translation="MTASDKWTERALRFLLSCVLFCWTQSAFAQRETGAPGPHADTTG
                     AQIVRADSSVAGNVSGFWPRATYLVTDGLTVSAGDTLVIEAGATILFQGPKDREKLSI
                     NCLGRLVVAGQDTAPVTFASAIPEGGAGRIWGGILYVGNMADGRVENAIFRNAEIGIQ
                     VRRGIIADRPAEADSANPIPEYILRRPTGPVVIRNCTIDSASFNGLVFVGVDSSVVAE
                     NNLIQNCSSGISCEEGARPILRLNRILNNTSTGIICSSESSPLITGNTVVGSSTAGII
                     CANSSNPTIDGCIIAKNGIGVSVTKSNPMITRCTIAKNEFSGIIAYEGAAPIVSSCNI
                     RGNGLSGIDNRSSGNITAERVWWGFITTDQRKAPVLDGGRRRERGEPVPEGQTGSVRI
                     ANSLSSPSLDAPGTPKQAQELILARDNAITNRFPERGEVSNGDTVWIQVRAIDESPYL
                     EDEAIIVFRSSSGGAAGTQRVLTESSASSGIYKGYVIASLAEGDPNIVVQVRNGDVIT
                     VETGTTPPLTARVVFVSKPPAIQNLRVDGQRRGARLTNPRPVFSWRYWDNENDPQRGT
                     ELELSTDSTFLTQHWKHAVSGPLSSYTFDGPTLTRGQTYFLRVRASDGYNFGRWSVTS
                     FHMNTPPPTPILASPEDGAILTDPRHKPVIAVRNVKDPDGDAVSYIFQAAYGEDFSSP
                     RLRAGGPEASQPVPEDPASDITVWRAMPTLYENTRIWWRAKATDGTEESEWSVPRSFS
                     LNTANDPPTPFVMLQPERDSTTNSLTPRLEWSHSYDPDPGETISFVVAFGRNKDLRDA
                     VIRHVEPVNEPVQSYQLTPSEMLLDNTWYYWTVRLEQDGRALFAANTAARDTTSVWRF
                     FVDTGNDPPVVAGIPPVVMTEDTPYKMNLGRYISDPDHPTDVLKVTVTGTEHLTAALG
                     PGNELTLTPAQNWFGGPERVRILVIDPILAEANGEISVSVTPVNDPPAVARVPDQSVA
                     EDTDLRVDLKPFISDVDNTASEMRWTATADTRKLTVSFADGVATIRGTQDWFGTTPVR
                     VTATDTGGLSASSEFNVTVTPVNDPPAVVQIPSIRTKEDEAAVVQLDNFVTDPDNQKS
                     ELRWSAVAEEPLTATIDPATRRATISAPPNWAGAERKVTFTVADPDGLSDRIVVAVGV
                     EAVNDPPTIAPIPEQRFNEDATLTLDLDPFVSDPDNTAREMTWTAVSGANIKAAVDAA
                     TRRVTFSAARNWYGGPETIRLTATDPGGLRATQSVNVSVISVPEAPVFARIPAVNIDE
                     DGQFVLALDEYLSDPDHRNDQLTVSYVAPQNVKVEFDRRTRRLTLSAPPNWNGGPETV
                     ALEAVDPDNERASATFSVTVRPVNDPPVLRAIPPVAFREDESTTLRLTEFVTDPDNTP
                     EQMQWVISGQARLKVQIAQGVATFSADKDWNGTETLTLKATDPGGLFAQTQIRVTVTT
                     VNDAPVVRQLPPVAFDEDGSSTVRLFDFVSDVDNTNEQLTWEVSGNNRVKVAIARGTA
                     TFTAEPNWHGTENLTITVADPDGLRASSSFTATVRSVNDPPVIARIPPVSFEEDGRTT
                     VDLAPFGSDADGDALTWTAASANPNLRVAVSGSVLTLSAAPDWNGGPVNVTVTATDPS
                     RGQATNTIPVTVTAVNDPPTLKALVPVTFPEDGTATLNLLNQVTDPDNTPAQMRWEAS
                     GGTNVKVRVAQGVATLTAEPNWHGSETLTITATDPGGLAARGTLSVAVSSVNDPPVIS
                     RLSAVSFDEDGSTTLDLAPFGSDADGDVITWTATSANPNVRVAVSGSILTISAAPDWS
                     GGPVNVTVTATDPAGAKATATIPVTVRAVNDPPELRELPSVNFNEDSTATLRLAQYVS
                     DKDNTPAQMNWQASGNTNVKVRFAQNVATFSAERDWSGSESVTITVSDPQGGSASRDV
                     RVTVRPVNDRPRVQPIPDATVEAGGQTEIDLAPFASDPDGDPLTWQVAGETGRITPVV
                     TGSTLRITAAAGAAGVATVNLVVRDPAGAQVTARVRITIQAAAPVQAPSPSGGGTTP"
     gene            19979..20851
                     /locus_tag="PLT86_03340"
     CDS             19979..20851
                     /locus_tag="PLT86_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35681.1"
                     /translation="MWWILFSVFVAPVLFLFLLVAVGALMPMRVGVSVQGQAQSAKEW
                     GGRATFGVRFFGGLIGLGFRAEGEASTRTHLAGGVLFWRWFFPLKSLDSIFARAPEVR
                     EVLSAPASATREPGVALVQPPPEKGPATRTRVEEDLETGGPADWTRLFEDSDLGDFPI
                     DWQRTSRPETTTAGVLADLRAAIARVWPFVREARRRFKGVVTLRFFRLTGSFGTGDPA
                     GTAWLLGTVYALAGAKGSADSVRLAGDFQRAIADGRCEVEWKVSTIRLWAAALALAWL
                     NWKHARALRTKKEA"
     gene            20866..21219
                     /locus_tag="PLT86_03345"
     CDS             20866..21219
                     /locus_tag="PLT86_03345"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOT35682.1"
                     /translation="MQTLLERLRDLVQTETVIGKPIEAGGVIIIPVSRVSVGFGAGGN
                     MNAQGAAAGAMKGGGGGGGVRVEPLGFVVISNGKAEVLPMKPDEPAINKIVDMVPGIW
                     DSVRSMLDKRDRKKD"
     gene            complement(21329..23203)
                     /locus_tag="PLT86_03350"
     CDS             complement(21329..23203)
                     /locus_tag="PLT86_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35683.1"
                     /translation="MPTLGHPLRSGKFGHTLVNGKPRRFRQSSHGSFLPCYDRTPRAG
                     FHIRTPTAQSGTGKKEKGRLHARSTALAIPAIICYSVSPTPYLNEHAANVKGLYDGFF
                     FQCGSWDSGVLTNIGVGNTAATNPNWLRLAGQNVAALKSVGCAENLLAISFGDSEPWP
                     SPETLLSGEFTEKLRQHFSALGKAAKATGFRGISIDTEYPYPRYSLDHPVYTYSDYTP
                     GDLMEASYRQGRVVMSAILGEFPEAVIFNLPGSLEGRPLARQFVSGLIEEMAARNAPG
                     GFHFGTEYAYSLHEPVTQAAIPRHEDAKMDRFLSGTSLEYWRSTCTMAPGVWPCHMVE
                     TGADDYRVRPWDEEMAELRQQMRILRSTTKRYMWSFSGQPIWLLPSAENRDRYGLSAT
                     YPGAEVFIPIWHDILRERVRYDQTPDADPRMIRLFDATKRFDEGRIDATEFCNAFGTP
                     AKWWALGVLSNPFVNPARTAKEALSMPISPSLVFFGRDGAVRWFPWAVQDNRGIVSMR
                     TFTDYLMTDNSSVHLATYVHSPRAVDAWLNLGWDDGLVVQVGDSVVFDRAEYPERGHG
                     AMYLDRYQFEAKVPIHIPAGVTRINVTTINAKGGWMYTFRITDADGYPLPDLRFSLED
                     "
     gene            23440..>24250
                     /locus_tag="PLT86_03355"
     CDS             23440..>24250
                     /locus_tag="PLT86_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT35684.1"
                     /translation="MVKTPIYMDNHATTPVDPRVLEAMLPYFTEVFGNAASRNHVFGH
                     EAEAAVEQMRGVIGAAVGAEPRNVIFTSGATESDNMAIKGVAEFYRERGNHVITQVTE
                     HKAVLDTCKELERRGYEVTYLPVDRYGLIDLDDLRKAIKSETILISIMMANNEIGTIQ
                     PVKEIGAIAKEKGVLFHSDATQAVGKLPVDVDELGIDLLSMSAHKIYGPKGVGALYVR
                     GKNPRVRLAPLFHGGGHERGMRSGTLNVSGIVGFGKALQIAVDEMESESARV"
BASE COUNT         4938 a         7167 c         7127 g         5018 t
ORIGIN      
        1 cgcctccggg aaagtaatcg gatgccacat tgacggcacg ctgggccatc tgttcgccga
       61 ccttgtcgag gccggtatcg actttcctga gtcagtggtt ccggcgccgg taggtgacct
      121 cactctcgag cagatccgcg acgcatcgag tccgaacacg ataatctggg gtgggatacc
      181 gtcggcaatg tttgcgccgc ctttcgagcg cgactgggtt ctgtcgtttg ccaagagagt
      241 ggtagcggtg ctgggcgagg agaggggatt ggtgttggcg ggcgccgatc aggttccccc
      301 gaatggtgac atcgacctgg tacgaaggat cggggacctt ctggaggaac tggggccgcc
      361 gacgcaaatc gggtcttgag ttggcaggca acctgcctga cgagacagga taagaacagg
      421 cgttctgttc cggtcgccgt tcagccggtt cccattgcaa acaaggccgc ctgctgttcc
      481 agcgttccgg cgaagcgttc cagagttgcc gccgtctccg ccagatcgtc acggagcggc
      541 gtctgccgca gccgggagac ctccacgcgg taccgggcag cctgttcacg cgatatggcc
      601 gccgcaaggc gcaagttctg ggacgtcgtc tgagcgtgat cagctatcgt ctgttgcatc
      661 gaagccgata cttcccccgg aggcgcccag cctgccgcga gacgagaccg ctccagtgcg
      721 gaacgcgtct gttccaatac cagtgctgac aacgtatcga gcaacaacgt ttccgtcgcc
      781 cggagtgccc ttctgacacg caggtatcga tctgcggcgg aaggaagcgg ctgcccgaca
      841 tacgccgcga ccgtttcaga gaagctccgc acggcagctc tgtaggcctc gatctgacgg
      901 cttacggaat ccatccgggt gacgtagcgc tggtattcat ccagttttgc cgcgtgttct
      961 tcacctgaaa gaagccgtgg atccacccct ccgaggtctg gaaggtccgg catccggatt
     1021 tgcggaggct gtggcgccgc cctggaaaga gctattcttg cctctccggg gaggctatcc
     1081 gcagcagttc gaagcatcac aacaacactg tcgaccattt ccggtgaccg gagcaggtgg
     1141 cgcaaccctt ccctcgacag cctcattgcg atctcgccgt ctcgccattc ctgccttcgc
     1201 cgccaggcga accgctccgc catccactcc tcggagcgca ccgtctgcgc gcctttgcgg
     1261 gcctggtcat gcgcctgaag gaaacccgag gggccaaaaa tgtaggggcg gaacacatcg
     1321 ttgaccattc tactctgtaa cacaaaatcg aagaaaggat ccgcaatcct tgcatcccgg
     1381 cgtgcgagat ccgtgacttt ctcccagaga agcggttctg cacgagccaa ccaccggctg
     1441 gcccggcgct caatgtaaat tgtctgcagc gcttcttcgc gcgcgacgag ggaatcccgc
     1501 tgctgcggta tggcggcgag ttgttcccgt gcaagggaaa cccactcgct cacgtcccga
     1561 tccccggtgc cacccgaaaa tccaagggaa tccgcgagat ggagtatctg ctggtgttct
     1621 ctgatcgcct gatccaatag ctgccgggag agattcaggt cgcgtatggt ctgctcgagg
     1681 agttcagttc ccggttgtcg ttccgcggcg tagtcgaggc cgccggtgtt ccattcctgc
     1741 agcgcatacg cgcggaaaaa gagcgcctca aacgttgccc gcgcgtagtg gaattccaac
     1801 gctctgttgt aggcttctct ggtacgaacg aggagcgccg cgcgttgcgc gctgtctgcc
     1861 gcgtaccgct ccaagcgcag aggggagtcc cggtaggcaa cgtgcgtcat cgcggcaagc
     1921 gaaagcgctg attgagctgc atagggaagc gcaagagaac gtgcgttccc tgaaagcctt
     1981 tcaaacgttg aaaggacatc cccgtacgcg ttttgtgcaa cgtcttcccg acctgagttc
     2041 gcggtgagct cgccaaggta aaacatgcgc tgtacgcgct ccaaattcgc acgtgtggac
     2101 cgcagtctgg cgagttccgc gtcgggattg aagccggaac tggagccctg cgccgatgcg
     2161 ggattcgcga ggaagcacga gatcaggacg gccacaatcg ccgccacgca gaagtgcggg
     2221 catccaaagg agatggaggg tatacccgaa gtccctgtca atggggcaat caggatccgg
     2281 tttcgcatac ctgcgccctc aaccggctcg cgcccgcagt tccgcgacct tcgcgcggat
     2341 ccgctgtgcg tcggataccc ccggtgcaag ctcgaggtag gtgctgtatt cgctcgccgc
     2401 ttcgcgaagc atctctctcc agtccgtatc cgcggcaact ccgtacacgt tgcgtgctgg
     2461 ttctccccgc agttccgccg aggcaaggcg aacaccgatg gcttcgtacg cgagtgcgag
     2521 gttgtaatgg tatatcgctt cgctcccttc cgcggcgatc gcgcggcgat aatactccac
     2581 ggcattcgaa agctcgctcc ggtagtaatg caccaggccg atgttgttca gaactcgtgc
     2641 ccactccgcg cgaatctcaa gggcacgacg gtactgctcc agagcctctt cgaaccgccc
     2701 ctgtcgttgg taggcgtatc cgagattgta ccgggagtag gcgttggcgc tatcgaggcg
     2761 gacagaggct tcgcattcct ggatcgcacg ttcgacatcg ccgctctgca catacatgaa
     2821 gcagagattg ttccggagtt caggcacgtc aggattgtac gtaatggctg attccagtgc
     2881 cgcggcacgc tctcgctcat cattcgagac cagggcgagg ccgttctgag cgaggagatt
     2941 tcgggaattc aattgcagtg cgcgacggaa actccgttcg gccagttctc gtttgccgag
     3001 acggtaattc gacatgccta tgagggcgtg cgcctcatcg gagtagggat tcaattcaag
     3061 cgcaaaccga aactgctcga tggcgtctgc aaatcgctgc gcttcatagt accgggttcc
     3121 aagatccaga tacgcgtgcg caaaatcgac gtcagaggca agttcaccac tctccccctc
     3181 aggtgcaggc gcaacacgga cctcaggtgc atggccgcag cccatcacga gcacgattgc
     3241 agccgcacta ctcaacaggc tctttgccac ccattttctt cgtctcagaa cgctcacctg
     3301 cagacattcc tccgtgggtg ctttaccagc ggcagtttcc gctccttata catacgccga
     3361 aacgtcgaat atctatcccg gttcacattg cacgcacctg gtccgaaaac gaaacccccg
     3421 ttacccagcc ggcaacgggg gtccagaaga tatggtgcgg aaggccggac ttgaaccggc
     3481 acgccctaac gagcatagcg ccctcaacgc tacgtgtcta ccaaattcca ccacttccgc
     3541 acgcggtgtg tctttgttac tgaggtcctc cgggggttgt cgtcggaacc ggcgcgggtc
     3601 ttccctgggg tgcctgcgac ggagcctgtg gactcggcgc aacctgggcg ggcgccgtct
     3661 ccgacaggcg gcgctgggcc tccgattgcc cttccggcac tgtgaccgcg cttttgccca
     3721 atatgctaat cacgacacac agcaacatga acgtcgctgc gagagcggtc gtcgtcttgg
     3781 tcagaaacgt ggcagctgcg cgtccaccca tgaactgtga ggatcctccg aacgttccgg
     3841 acaatccttc gccttttccc gattggagca agatggacaa catcaggaac agacagaccg
     3901 ccacaaaaag gattttcaaa aatatcatgt tggcacgcct cttttccaag tccggcgtct
     3961 ctcggtggtc agacagccgc tttcactata gccgcaaagg agtccgcctt gagcgcagct
     4021 ccaccgatga gcccgccgtc tatgtccggc tttcccataa gctctttcgc gttttccggt
     4081 ttcatgctgc cgccgtactg aatccggacg gtgtctgcaa ttgccgagtt cgagatttgt
     4141 ttcagaacgt tgcgaatgaa cgcgtgtact gcctgtgcct gatccggagt ggccgttttt
     4201 cccgtgccga ttgcccatac tggttcgtac gcgatcacga ctttgcccac ctggtccggc
     4261 gaaagcccca gaaggcctcc cgtaacatgc tccttgacga tcgtttcggt ctggccgtct
     4321 tcccgttcct cgagcgtttc acccacacag aggataggcg taagtcccgc agccaacgcc
     4381 gctttcacct ttttgttgat ttcggagttt gtctcgtgga agtactggcg gcgttcagaa
     4441 tggccaagga tcacgtaccg gcacccgaga gtgagcagca tttccgcgga cacctcgccg
     4501 gtgtaggccc cattcttttc ccagtgcatg ttctgtgcgc cgagttcgat attcgttccc
     4561 acgagcgccg gtgcaacagc agccagcgcg gtgaacggcg gacacacgac gacgtcaaca
     4621 ttgctgacgt ccgacacgag aggccgcagt tcgcccacga gagcagccgc ttctgcagcc
     4681 gttttgttca ttttccagtt gcccgccacg attcggcgtc gcatgcagaa acctcccgac
     4741 gtgaaaaccc gggttgaaat ctaccgggat tgggttaccc tgaccgcaaa acaggatttt
     4801 ggaagatttt aataatacgg gatgatgtcg ctgatgcgca atacccgcgc catttccttc
     4861 acacgccaga tcgcgctgtg gagcgcgtga agccggaaat acagagaagc gtcgcccgta
     4921 ccgttgagcg acgcttctcc gacagagcgc tgacgagacc tatttctgag tgagagccgc
     4981 tactcccgga agtactttac cttccagata ctccaatgaa gcgcctcctc ccgtggaaat
     5041 atgcgacatc cgttccgcca acccggcctg ggtgatggct gccgcggagt cacccccgcc
     5101 gatgacgctg atagatccct tgtcggttgc ctctgccacc gcatgtgcga tggcggctgt
     5161 acctttcgcg aaattcggca tttcaaacac accaagcgga ccgttccaca cgatcgtttt
     5221 tgccgatttg attatgtccg agaactcctt gaccgtcgca gggccgatgt ccacgccctc
     5281 ccagccggca ggaatctcgt ctcgttttgc gacttttgtg gtggcattgt tgtcaaaggc
     5341 gtcggcaatt actgtgtcca ccggcaggac gattttcttg cccgctttgg aaagcaattc
     5401 ccgcgcgagg tcgattttgt ccgcttcgag gagcgatttg ccgatctcca gacccatagc
     5461 cttgtaaaac gtgtacgcca tcccgccacc gataagaagc gcgtcaacct ttggcaagag
     5521 gttgttgatc acgtcaatct tcccgctgat tttggacccg cccatgattg ccacaaaggg
     5581 ccgcgcaggt ttttccaacg cctcgccaag gtatttgagt tccttttcca tcaagaagcc
     5641 tgccgcgttc tgcttgaaat gcgcagtgac gcccaccgtt gacgcatgcg cacggtgagc
     5701 ggtgccgaac gcatcattca cgtacacatc gcccagcttc gcgagttttg cggcaaactc
     5761 gggatcattc gcctcctctt ccttgtggaa acggaggttt tccagcaata cgacatcgcc
     5821 gtctttcatt cgtcccacaa cagcttccgt ttcctcgccc acgcagtctg gcgccagcac
     5881 aaccggtttg cccagaatct ccgccaacct tttcgcaacc ggcgcaaggc tctgttttgg
     5941 atcgggcttg cctttggggc gtccaaggtg cgccataagg ataagcttgc caccgcggtc
     6001 gatgatccag cggaccgttt ccagagttgc ctgaatccgt gtttcgtcgg tgatgttctg
     6061 gttttcgtcc aacggaacgt tgtagtcctc gcggacgagg acacgcttcc ctcgaacgtc
     6121 tatgtcacga agagtgagtg ccgccattga gtggtctccc cgatggatgc tggttttccc
     6181 gaaacacgag gggcgcaccc cggtgcgccc ctcacatgat acactctgtt acgcatttcg
     6241 tgtgcggggc ttacgacacc cacattttcg cgaggtcaac cacgcggttc gagaagcccc
     6301 attcgttgtc gtaccacatg cacactttga caagcgttcc gccaagaacc atggtgcact
     6361 tcgcgtcaaa gatgctgctg tgaggattcc caacgatgtc gctgctgacg atcgggtctt
     6421 ctgtgtactc gagaatgccc ttcatcgggc cttccgccgc cgctttcacg accgcgttca
     6481 cttcttcctt tgtggtcgct ttgcccacga caacggtgag gtccgtcatt gatccgtccg
     6541 gcaccggaac gcggagcgcc tgaccatcca gcttgccttt gagttcggga atcacctcgc
     6601 cgattgcgcg ggcggcgccc gtagtcgtcg gaatgatgtt catggcgccg gcgcgggccc
     6661 gacgaaggtc gctgtggttg aagtcaagga tgacctggtc gttggtgtac gcatggacgg
     6721 tcgtcatcat tccgctgacg atcccgaacg tatcgttcag gattttcgcc accggagcga
     6781 gcgagttcgt cgtgcacgat gcgttcgaaa ggagcttcca ggacttgtcc aatgtcttgt
     6841 cgttcacgcc gacaacgatg gtgccgtcaa gcttcccttt cgcaggggcg ctcaggatga
     6901 cacgcggtgc accggcgtcg aggtgcatct tgagctgttc cgcgtcggtg aaacggccgg
     6961 tggattccaa cacgaggtcc acgccaagcg ccttccacgg caatttggag ggatcctttt
     7021 ccttgctgac cggaatcttc ttgccgttga cgacgatgtt ttcgccttcg gatgagacgt
     7081 cgcccgggaa cctgccgtgg atagaatcgt acttgagaag gtgagcaaga gtcgccgcgc
     7141 ttgtcagatc gttgatcagg agtgtgtcca ccccgccttg cgccaccgca gcgcgaaaca
     7201 ccaagcgccc aatgcgcccg aacccgttga tacccagttt gatcgccata gtatcctcca
     7261 aatttgcgtg tgagcccgaa atacaaacct tgagagcgcc cgacaggcag tgtgatcagc
     7321 cgaagaattt cttcgccaca tcgagcctgt cagtcggaac cgttttcaat tcacccactg
     7381 catccgcaag cggcacggct accatctcgc cgccgcgcaa tgatgccatc ttgccgaact
     7441 cgccagcgtt aatcatgtct acgactttga caccgagtcg cgttcccagg acacggtcga
     7501 acgcagtggg atcgccgcca cgctggagat ggccgagctg gatgtggcga gtttcaatcc
     7561 cggtgcgttc ctgcagttct tccgcaacta cttcggcgat accacgcccg cttccgaggc
     7621 ggatgtgacc gaatgagtcc ttttccgcgg acctctgaag cttggcggtg agcttgggat
     7681 cttcagcccc ttcggcgatt gcgaccatgg cgtatttctt cccggccgcg taccgttccc
     7741 tgcacagttt ctcgatctcg tccgtatcga atgggacctc gggaatcgcg atgtagtggg
     7801 ctccgccggc cattccaccg tgaagagcca tccatcctgc gtggcggccc atgatttcaa
     7861 caaccataat gcgctggtgc gcttcggttg tagtgtgaag acggtcgagc gcttccatca
     7921 cgcgattgat tgccgtatca aacccgaacg tgtaatcggt cgcgttcaca tcgttgtcga
     7981 tggtcttggg aactcccacg acgtttccac cggcgtccgc gagtttcttc gccacgccga
     8041 gcgtatcttc gccaccggcg gcaacgaggc agtagacgtt gagatcggcg agattgcgct
     8101 tcacgatctc aaacccatct ttcaccttgg ccacgttcgt ccgcgacgag tagaggatgg
     8161 ttcctccctg acggtggata tcgtacacac gttctttcgt aaggatttcg taatcctttt
     8221 cgagcatccc cgcccagcca cgcaggaagc cgagtacctc atatccggag tcaattgcac
     8281 tcaccgtcac tgctctgatt accgcattca ggcccggcgc atctcctccg ccggtcagga
     8341 ttccaattcg cttcatctgc ttctctccgc tgtccttcgg tggggtgtgg cgcgcgggtg
     8401 tgaggattct accgtgcaac ctcggcaaag acccggcgtg taccgtgaaa aggcggtttg
     8461 ccgccgccgt agaaatgcct cagatcagag ttttaaggta cagcgagcgc ggaaatgggt
     8521 caaggcggcg acgcgaagga ttagtacgct gagatcctgt ggtaccttct cctcgacttt
     8581 aacgtttgat gggattcagc gcgtcatgtg gatgactgaa gtccagagtt acccgtacgt
     8641 tttgccaact ggtgtttcag gatcgtgtaa cggaacgcgc caggtttcca aggggcacta
     8701 ctgaattcat ctggaatgca gtcttgatca atccccaatc atcaccagcc gttctttcgt
     8761 cacgcgctga ttgctcttca aatctcttac ggtgattatc agggtgtatt cgcctttcgg
     8821 tataccggag gtagacagtt ggaaatgttg gtactcggat ccgcgaatcc cctccatgac
     8881 gcgtgctaca cccacccggc gggagctgcc accgagtatt ctgccgatgc tgccaaggat
     8941 gctgccacct tcgccagcac cggtgcggaa tgcgtgctcc acttcgtatt ctgtgtcgcc
     9001 gtagtccgtg ccacgtgtga gattgtagat ttcaaaatac acgtggatag gttgccctcg
     9061 tctgaacgag cgagaagcca tcgggagaat gtgcagcccg tttcggacga actttcgcgc
     9121 gctggtgcta tcagcggggc ggatggaacc tgccaccacg atgtcactca tggccagagt
     9181 cgagctgtcg aatttcgcga tcggcacgtt ttcgcggtac gcctggagtc gtcccgagcc
     9241 gacgtcctcc gcctgcacgg aaaggagcac gcgctgcccg ccgtcgagca ccattgatcg
     9301 ggtgtccacg tggatgctac ctctgtcctg gctggggcgg gtcggcgaga tcaggtccgt
     9361 tcggtcaaga aaacggccct gcacattcca caacgtgtcg aaaacggcaa acccgttctg
     9421 tacggtcgct ttgaaggttc cgtcgtcctg ggcacggaat tgcagatccg ccatagggag
     9481 cccgtaataa atgtcaacct cggttttgct tgttgtgggc tcccgaaact gcgcgacgta
     9541 gtagtagaaa ttgaaaggag cctgctgcgc gtcatactcg aattgttccg gaacctccat
     9601 cgcggcgatg gtcaactgct cctgcggtgc gtattgctgc atcacagtgg ccttattcat
     9661 gtcagttgtc ggcggaggcg tggcaaaccg gaaatccccg cggccgattc ggtccacgaa
     9721 cgtgagattg agcccattca gaactccggt gtacgtccat tcctcccacc tgatgagctg
     9781 tgcctgcgcc atggcgctga acgtgggcac ctgctccctc gcctgttgcg ttgacgtcac
     9841 agcggatgca gccgcttcat cgctgcctgt gtcgccggtt tcgcccacat ctccgtactg
     9901 ctgaccggtt gttgacgtag actgcgtcgg ttggcccgac tcgaaacccg attgcagcat
     9961 ctgaacgggg tccacgagag gaaacaccgg cagaacaccc cgttgaagac tttccggagg
    10021 gttcgtcccg tagatcgcgt tcatgtatcg ctcgcgaacc atatctacgt tatgattcgt
    10081 gctgaagttg gggttacttg atgaagcccg ctggtcgggt tctccgtaac ggatgtacac
    10141 ctgtcccctg tcgtcccacg gctgccttcc ccgcgagaaa tgacgttttg cataccacac
    10201 gcgacggaaa tgctccagca ttcgttcgtt caccgcggtt gccggcgtcg gatcgcgtct
    10261 gagccagaac ttctgaagga aaatggcctt ctgttcgccg gaaacgcgtt gcacatacgc
    10321 agcttcctga gggctcgtga taaggctgat atcctcgtaa agagcgcgtt cgtcttccgg
    10381 aagaagctga agagcctgca tgtacgcctc gtgtgcccgg gcaaattgcc tgtcgcccat
    10441 gtaggttgca gcaagcgcca gactgagttc cccctctttg ccggacgtgt tgagcaacgc
    10501 gtcgtaaaga cgctgtcgtg catgccagta atgctgtttg tccagaagcg tgtaccccag
    10561 ccggatcatg gtttccccgt gctggggatt cgcacgtaac tgggcttcat agctgatgat
    10621 cgcctcgtcc agttcccccg aatcctcctg aaggcgcccc agttcgtagg cagcggaagg
    10681 atgattgggg gccacttcca gcacgcgcct gtatgttcgc ttcgccgcaa acctgtcgac
    10741 gaaacctgat gcctggatgc gcgcgacctc aagcaaggca tcagcaaacc ggttgttttt
    10801 gcgaaacgct tcctggaagc gcgtcttcgc ccggtctctc tgcccgcgtt ttttgagaat
    10861 caggccttcg ccaaactgct tgtaaccctt gttgcggtcc cagctttccg cctgttcaaa
    10921 caagcgtttc gctgcatcga tgctatcttt ggaaaccagg atacggccaa gcccaaccgc
    10981 cgcaagcgcg aaacggggat tgatgcggag tgcggttcgg tacaggacct ccgcactgtc
    11041 agagtctccg ttcgccaact ggacggcagc gtgatttacg agggaggtcg cttcgtcgtc
    11101 ctggccccgg acgtgcgaag aacacaccag gatgatcgtg agcaagatca acgcggtaac
    11161 tttcgttctc atccagaaaa tctcccggat cgacgtttca cacccaccag tttcgagaac
    11221 acccagacac actcagttga agcgcaccag ccgccgaatc ccgaatcccg ccgacatata
    11281 gaagacaccg gtgaagcctt ctccctgccc gacagactcg tgccaaaggc tgagaagcaa
    11341 ggtcgtgtcg ttgaccgcac tcaacacgtt ccgcggtaca acaacggaat cgtcctctgc
    11401 ggcggctgta gtggtccaga gtgtgtcacc gagcgcgcca tttgcggaaa gcctgtgcag
    11461 ttgcagaccg acgcctcccc cggtgatccc agaccaacgc accacaatgg gttcatcacg
    11521 attcagatag cttcccgctg ccggagaaac catgacggga cgagacgcgg gcaaccctat
    11581 cacatttcga acgctgcgcc atgtcgtcga tacatccacc tgaaggattt ccgctggttg
    11641 cagtggaatc gaatttccga agagggcctg attccccgcg gtcaacaccc ggggaactct
    11701 tgttccgttc acggttactg cagcattgtc ggacactccg tcgccaaaca agtacacgct
    11761 tatggtatcc aacggtgagc tggcagtccg ccgttcgtca acaatactgt acgcacggaa
    11821 atccccgtac agcgggagag gactccgcga cgcaaacggg cgattctcgc acccatcgac
    11881 gcactggatg acaaatgcca gcgtcgcaac cgacggtgcg actgcgtaca ggcgccgttt
    11941 cgaatatgtt gcggcgttct tcctcggccg aacacggggc aggaagagaa gagcgcgtat
    12001 gcacagtctc gtggaacgca tggtatgaat aacgtgccaa atcagcgaaa accccaagga
    12061 gtgcgatacg gggttgcccc acacgtcctc gggattatac cctcgtagac gacttcaggt
    12121 ttcctgagaa cacggatttg agggcttcag ataacatgcg aaaaggatca aagttcttcc
    12181 ttgcaggttg cataagtatt ggattgttcg cgacctgcat cgtggacgcc ttcgctaccg
    12241 aaacgccatg gcgttgcatt gcggcgtcac tggactccat cgtccaacgg cgggctcttg
    12301 ctcccgcaca ggtaggaatc gccgtctggt catttgatcg acgtgagtgg gtgtacgaga
    12361 aaaacgccgg tcggctcttc gtgcctgcgt caaacgccaa agttctcatc ggggcggctg
    12421 cgctgcacac tcttggccca gcgaagacat tcgccaccac acttacggca gatcgggttc
    12481 cgatttcggg cgacagcgtg cttacgggca acatttacct gtacggcggc ggaaatccgg
    12541 acctcgtaac tggtgatctc accggattcg cggagacatt gcgcgcctcc ggagtacggc
    12601 ggatacgagg tgctgttgtc gttgacgctt cccattttga ctccgtgaat tatgcgttgg
    12661 gatggtcgtg ggaggaccgc ccttaccctt attgtccccc gatcagtgcc ttcctgctca
    12721 atgggaacgt cgttcaggtt acggtgcgcc cggggtcggc ccccggccga ccagttcacg
    12781 tgacgctcgt gccgaaagat gcgggaatcc ctgtttccgt cgcagccaga acaggaagcg
    12841 aacacaacac gtcgtcacgg atccgtgtgg aacggtccaa ttccgggatc gtcgtcaagg
    12901 gcacgatggg cggacgcgcg gagccgataa ctgtctggcg gacggttccg gatcccgtcc
    12961 gatacgccgg ttcggtgttc atgggtgcac ttcaggatgc cggaatcacc gtggagcgag
    13021 gcatgtcgat cggcacctcg ccaaggaatc tgccggtgct tgcgcgtgtg tactcggatc
    13081 cgttggatgt gattcttcgc cgatacttga agaggagtga taacctcctt ggcgaaacgc
    13141 ttgtcaaaca aatgggtgtt gccacgagcg ggcgtggaac ctgggaagat ggtctttccg
    13201 ccgtgaggcg cgcgttgcgg gatatcgccg gtcttgattc gaacgcgtac cgactggcgg
    13261 acggctccgg cctcagcagg ctcaacgaag tatcaccgcg gatgctggtt caagtaatca
    13321 cttccgccag cagttccttc agtctggctc ccgagtttct ttccgcactt tctaccgctg
    13381 gagtggacgg cacgctttca catcggatga cggaagacga gacgcgggcg taccttcgcg
    13441 gcaaaaccgg cacattgagt ggcgtcagct cgctttccgg cgttgtcgaa agccgttccg
    13501 gcgcgagatt cgcgtgcgcg atcatgatga atggcacctc tccatccgca cagacaatgc
    13561 gccaagcgca agatgacgtt gtgcgcgttc ttcggaattg catcgagcag tgacattccg
    13621 aacgcctgcc atcctcttgg gggtcgggcg taatccccgg cggacgggaa aagaaccccg
    13681 aatttcgtaa cttgtaacgt gcgcgcgtcc gcaccagata tgatctttgt cattttctgc
    13741 cttgtacttc tattgaactt ccttgacctt agctctcgcg cgcacgaact tactactgtt
    13801 gtattttgtc ccgaactcaa aatgacggac cccttcccgc gggaagggaa tgggtgcgga
    13861 atccggcgca cgttctggca gggggtactc agggatgact gcaagcgaca aatggacgga
    13921 gcgagcgctt cgcttcctgt tgagttgcgt ccttttctgc tggacgcagt cggctttcgc
    13981 acagagggaa accggcgcac cagggcctca cgccgatacc actggagccc agatcgtcag
    14041 ggcagattcg tctgtcgcgg gcaacgtttc tggattctgg ccacgcgcaa cctaccttgt
    14101 caccgacggt ttgactgttt ctgcaggcga tacgctcgta attgaggctg gggctacgat
    14161 tctgttccag gggccgaagg acagggaaaa actcagtatc aactgcctcg gtcgactcgt
    14221 tgtagcaggc caggacacgg ccccggtcac atttgcgagt gccattccgg aaggcggggc
    14281 cgggaggatc tggggaggaa tcctttacgt cggcaatatg gccgacggcc gtgttgagaa
    14341 tgccattttc aggaacgcgg aaatcgggat tcaagttcgt cgcggtatca ttgcagaccg
    14401 gcctgcagag gctgattctg cgaaccccat ccctgagtac atactcaggc gtccgaccgg
    14461 ccctgttgtc atccgcaact gcacgattga cagcgccagt tttaacgggc tcgtgtttgt
    14521 cggtgtcgac agcagcgtag ttgccgagaa caatttgatc cagaattgct caagcggcat
    14581 ttcgtgcgaa gagggcgcga gacccattct tcgcctgaat cgcatactca acaacacgag
    14641 caccggaatc atctgctcgt cggagtcgag cccgctgatt accggcaata ccgttgttgg
    14701 aagctctacc gcagggatca tctgtgcgaa cagctcgaat cctacaatag acgggtgcat
    14761 aattgcgaaa aacggtatcg gcgtttcggt gacgaaatcg aacccgatga tcacgcgctg
    14821 tacgatcgca aagaacgaat tcagcgggat catcgcgtac gaaggagcgg caccgattgt
    14881 ctcgtcgtgc aacatccgcg gaaacgggct ctccggcata gacaaccgat cctcgggcaa
    14941 tattacggcg gagagggtct ggtgggggtt catcacgaca gatcaacgaa aagcacccgt
    15001 gctggatggt ggaaggcgca gggagagggg ggaaccggtt ccggaagggc agaccggctc
    15061 ggtacgtatc gccaattcgc tttcctcacc ctcgcttgac gcgccgggca ccccaaaaca
    15121 ggcgcaagag ctgattctgg cacgtgataa cgcaattaca aatcgattcc ctgagcgagg
    15181 tgaggtatcc aatggcgaca ccgtctggat ccaggttcgt gccattgacg aaagccctta
    15241 cctcgaagat gaagcaatca tcgtgttccg cagctcttcg ggtggcgcag caggaacaca
    15301 acgcgttctg accgaatcga gtgcttccag cggcatatac aagggttacg tgatcgcttc
    15361 cctcgcagaa ggcgacccaa acattgtggt acaggtgcgc aacggtgacg tgataactgt
    15421 ggagacaggc acaacccctc cgctcactgc gcgggtagtg ttcgtgagca aaccaccggc
    15481 aatacagaac ctcagggtag atggccaacg acgtggagcc cgattgacaa atccgagacc
    15541 cgtgttctcg tggcgctact gggacaacga aaacgaccct caacgcggca cggagctgga
    15601 actctcaaca gattcgacat ttctcactca acactggaag cacgcggtaa gtggtcccct
    15661 cagttcatac acgttcgatg ggccgaccct gacgagaggg cagacgtatt tcctccgtgt
    15721 ccgggcgagc gacgggtaca atttcggccg ttggtcggtg acgtcattcc acatgaacac
    15781 cccgcctccg acacccatcc tcgcctcgcc tgaggacggc gcaattctga ccgacccgcg
    15841 gcataaaccg gttattgccg ttcgcaacgt caaggaccca gacggcgatg cggtcagcta
    15901 cattttccag gctgcgtacg gagaagattt cagttcgcca aggttgcgag cgggtggccc
    15961 ggaggcaagc caacccgtgc cggaggaccc ggcttccgat atcactgtgt ggcgtgcgat
    16021 gccgacgttg tacgaaaaca cccgcatctg gtggcgtgcg aaggcgacgg acgggacgga
    16081 ggaaagtgag tggtcggtgc cgcggtcgtt ctccttgaat acggcaaatg acccgcccac
    16141 gccgttcgtc atgctgcaac cggaacgtga cagcaccacc aattccctta cgccgcgatt
    16201 ggaatggtca cactcgtacg atccggatcc gggagagacc atttcgtttg tagtggcatt
    16261 tggaagaaac aaagacctca gagatgcggt gatccggcac gttgaaccgg tcaacgagcc
    16321 agttcagtcg taccagctta ccccgtcgga aatgttgctg gacaacacgt ggtattactg
    16381 gaccgttcgg ctggagcagg acgggcgggc cctttttgcc gccaatactg ctgcacgcga
    16441 cacaacgtcc gtgtggaggt ttttcgtcga caccggcaac gacccgccgg ttgtggcggg
    16501 aatcccgcct gtcgtgatga cagaagacac gccctacaag atgaacctcg ggcggtacat
    16561 aagtgacccg gatcacccca ctgacgtgtt gaaggttacc gtcacgggga cagaacacct
    16621 gaccgcggca ctcggccccg gcaatgagct gactctgacg ccggcgcaga actggtttgg
    16681 cggtcccgaa cgagttcgca tactcgtgat agacccgatc cttgcggaag ccaacgggga
    16741 gatctctgtg tccgtgacgc cggtgaacga cccgcctgcg gtggcgcggg tcccggacca
    16801 atctgtcgcg gaagacaccg acttgagagt cgatctgaaa ccgttcatat ctgacgtaga
    16861 caacacagca tcagagatga ggtggaccgc aaccgcagac acgcgcaagt tgacggtctc
    16921 ttttgctgat ggcgttgcca cgatccgggg cactcaagat tggtttggaa ccacaccggt
    16981 gagagttacc gcgacggaca cggggggcct ctctgcatcg tccgagttca acgttacggt
    17041 caccccggtc aacgacccgc cggcggtcgt acagattccg tcgatcagaa ccaaagaaga
    17101 tgaagcggcg gtcgttcaac tggacaactt tgttactgac ccggacaatc agaaaagcga
    17161 gttgcgatgg agcgccgtgg cggaggagcc gctgacggcg acgatcgacc cggcgacaag
    17221 gcgggcgacc atatccgcgc cgccgaactg ggccggcgca gaacgcaaag tcaccttcac
    17281 cgtcgcggac ccggacggtc tgagcgacag gatagtagtc gctgtcggtg tggaagcagt
    17341 gaacgatccg cccacgatcg ctccgatccc tgaacagcgt ttcaatgaag atgcaaccct
    17401 gacgctggat ctcgacccgt ttgtctcaga cccggacaac acggcacggg aaatgacctg
    17461 gaccgcagtg agcggcgcaa acatcaaggc ggcggtggac gccgccacaa gacgcgtcac
    17521 gttcagcgcg gcgaggaact ggtacggtgg accggaaacg attcgcctca cggcaactga
    17581 tcccgggggg ttacgagcga cgcagtcggt gaacgtcagc gtgatttccg tgcctgaggc
    17641 cccggttttt gcgcgcatac ctgctgtgaa tatcgacgag gacgggcaat ttgttctcgc
    17701 gctggatgag tatctctcag acccggacca tcgaaacgac cagctgaccg tgagttatgt
    17761 cgctccgcag aacgtgaagg tggagttcga caggagaacc aggcgactta cgctttctgc
    17821 accgccgaac tggaatgggg gcccggagac agtcgctctg gaggcggtgg atccggataa
    17881 tgaacgcgca agcgcgactt tttctgtcac cgtaaggcct gtcaacgacc cgcccgtcct
    17941 ccgcgcgatc ccgccggtgg cgtttcgtga ggacgagtca acaacattgc gactgacgga
    18001 atttgtgacg gacccggaca acacgccgga gcagatgcaa tgggttatct cgggacaagc
    18061 cagattaaag gtccagattg cgcaaggcgt tgccacgttc tccgccgaca aggactggaa
    18121 cggcacggaa acgctgactc tcaaggccac tgacccgggt gggttgttcg cccagactca
    18181 gatccgcgtg accgtgacca cggtgaatga cgcgcccgtt gtgaggcaat tgccgccggt
    18241 cgctttcgat gaggacgggt cgtcaacggt gcggttgttc gatttcgtca gcgacgtgga
    18301 caacacaaat gagcagctca cgtgggaagt atcgggcaac aaccgggtca aggttgcgat
    18361 tgcgagaggg accgcgacct tcaccgcgga accgaactgg cacgggacgg agaacctgac
    18421 gataaccgtg gctgatccgg atgggttgcg cgcgtcaagt tcgttcacgg caacggtccg
    18481 ctcggtcaac gatcctccgg tcatagcccg catcccaccg gtatcctttg aagaagacgg
    18541 cagaacgacg gtcgatcttg cccccttcgg ctctgatgcg gatggggatg cccttacgtg
    18601 gacagccgcc agcgcgaacc cgaatctcag agtcgccgtt tccggtagcg tgctgacgct
    18661 cagtgcggcg ccggattgga acggcggacc agtcaatgtc accgttacgg cgacagaccc
    18721 gtcgcgaggc caggcgacga acaccattcc cgttacggtg acagcggtga atgacccgcc
    18781 cacgctgaaa gcgttggtac ctgttacctt cccggaagac ggcaccgcca ctctgaacct
    18841 cttgaaccag gttacggacc ccgacaacac ccctgcccag atgcgctggg aggcttcggg
    18901 cgggacaaac gtcaaagtca gggtggctca aggcgtggcg acgctgaccg cggaaccaaa
    18961 ttggcacggt tccgaaacgc tcacgattac cgcaaccgac cccggaggtt tggcggctcg
    19021 gggcacgttg tccgtcgccg tcagctcggt gaacgaccca ccggtgatca gccgcctgtc
    19081 tgcggtctcg tttgacgaag acggcagcac aacgctcgat ctcgcaccct ttggctccga
    19141 tgcggacgga gacgttatca catggacggc gacttccgcc aacccgaatg tgagggtcgc
    19201 ggtctccggc agcatcttga ccatcagcgc tgcaccggat tggagcggcg gccctgttaa
    19261 tgtgacggtc acggcaacgg atccggccgg cgccaaggca accgctacga ttccggtgac
    19321 ggtgagagcc gtgaacgatc ctccggaact ccgtgagctt ccatcggtga atttcaacga
    19381 agacagtacc gctacgctgc ggctggcgca gtatgtctct gacaaggaca acacgcccgc
    19441 gcagatgaac tggcaggctt ccggcaacac gaacgtgaag gtccggtttg cgcagaacgt
    19501 cgccacattc agcgcggaac gcgactggag cggctccgag tcggttacca tcaccgtgtc
    19561 cgacccgcag ggcgggtccg cgtcgcggga cgtgcgggtc accgtcaggc cggtgaacga
    19621 ccgtccccgg gtccagccaa ttccagacgc aaccgtggag gcgggcgggc agacggagat
    19681 cgatctcgca ccgttcgcca gtgacccgga cggagacccg ctgacgtggc aggtggccgg
    19741 cgaaacggga cgaatcaccc cggttgtcac gggttccacc ttgcggatca cggcggctgc
    19801 aggcgcggca ggcgtggcaa cggtgaatct cgtggtgcgc gaccctgccg gagcgcaggt
    19861 gactgcgcgg gtacggatca caatccaagc ggcggcaccc gtgcaggctc catcaccgag
    19921 cggcgggggt actactccgt agtggaattc tgattgcgct tacaagggga caggaccgtt
    19981 gtggtggata cttttcagcg ttttcgtcgc accggtcctg ttccttttcc tcctcgttgc
    20041 ggtcggtgct ctgatgccaa tgcgagtcgg agtcagtgtt cagggtcagg cgcagagcgc
    20101 gaaggaatgg ggaggacggg ccacatttgg tgtacgtttt ttcgggggcc tgattggtct
    20161 gggcttccgg gctgaaggtg aggcatccac ccgaactcat cttgctggtg gggtgctttt
    20221 ctggcggtgg ttttttccgc tcaaatcgct ggattccatt ttcgcgagag cccctgaggt
    20281 cagggaagtt ctgtctgcgc ccgcatcggc gacacgtgaa cctggcgtgg ccttggtgca
    20341 accaccaccg gaaaaaggcc cggcgaccag aacacgagtc gaagaggact tggaaacggg
    20401 aggccccgcc gactggacac gccttttcga ggactctgac cttggagatt ttccgattga
    20461 ctggcaacgt acaagccgcc ctgaaacgac aacagccggg gtgctcgcgg atctgcgagc
    20521 ggcgattgcc cgcgtctggc cttttgttcg ggaggcacgc cgtcggttca agggcgtggt
    20581 cacactgcgt tttttccgcc tgaccgggtc gttcggcacg ggcgatccgg cgggcacggc
    20641 atggctgttg ggaacggtgt acgccctcgc cggggccaaa ggttccgccg attcggtgag
    20701 gcttgccggt gatttccaga gagcgattgc agatgggcgt tgcgaagtag agtggaaagt
    20761 aagcacgatc cgattatggg cggctgcact cgcgcttgcg tggctcaact ggaagcacgc
    20821 acgcgcatta cggacaaaga aggaggcctg acaatcgaag ccttgatgca aacactgctg
    20881 gagcggctgc gcgacctcgt gcagacggaa acggtcatcg gcaaaccgat tgaagcgggc
    20941 ggggtgatca tcattccggt ttcgcgcgtc tcggtcggtt ttggggcagg tggcaacatg
    21001 aatgcgcagg gcgctgccgc cggagcgatg aaaggcggag gcggcggagg cggagtgcgc
    21061 gtggaacccc tcgggttcgt cgtcatcagc aacggcaaag ccgaggtgtt gccgatgaag
    21121 cccgacgagc ccgccatcaa caagatcgtg gacatggtgc cgggcatctg ggattcggta
    21181 cgttcgatgc tcgacaagcg cgacaggaaa aaagactagg aacctgaaac ctgtgcaaag
    21241 tgtgcctgca cccgggtgca ggcacacttc agtctccgcc ggagagtccc gcggcgtacc
    21301 tccttgccgc acaccgcacg accccccgtc aatcctccag gctgaatctc agatcaggaa
    21361 gtggatatcc atcggcgtcc gtgatgcgaa acgtgtacat ccatccaccc ttcgcgttga
    21421 tggtagtcac gttgatgcgt gtgacccccg cgggtatgtg gatggggact ttcgcctcga
    21481 attgataccg gtcgaggtac atcgcgccgt gcccacgctc agggtattcc gcgcggtcga
    21541 acaccacgct gtcgccaacc tgcacaacca gcccgtcgtc ccacccgagg ttaagccatg
    21601 cgtcgaccgc cctgggcgag tgtacatagg tggccagatg cacactggaa ttgtcagtca
    21661 tcaagtaatc tgtaaacgtc cgcatggaaa cgatgccacg gttgtcctga accgcccagg
    21721 ggaaccacct gaccgccccg tcgcgcccga agaacaccag agaaggagag atgggcatgg
    21781 agagcgcttc cttcgccgtc cgtgcgggat tgacaaaggg gttgctcaga acgccgagag
    21841 cccaccactt tgccggggtg ccaaatgcgt tgcagaattc ggtagcatcg atccgtcctt
    21901 cgtcgaatcg cttcgtggca tcgaacagac gtatcatgcg cggatcagcg tccggagtct
    21961 ggtcgtaccg gactcgttcc cgcaggatat cgtgccatat cgggatgaat acttccgctc
    22021 ccggatacgt ggcggagagt ccgtaccggt ccctgttttc ggcggacggc agcagccaga
    22081 tgggctggcc gctgaacgac cacatgtagc gtttcgtagt cgaacggagg atgcgcatct
    22141 gttgccgtag ttccgccatc tcctcatccc acggtctcac ccggtaatcg tcagcgcctg
    22201 tttccaccat gtggcagggc cagaccccgg gtgccatcgt gcacgtgctc cgccagtact
    22261 caagactcgt ccctgagagg aaccgatcca tcttcgcgtc ttcatggcgg gggatcgcgg
    22321 cctgggtcac aggctcgtgg agcgagtacg catattccgt tccgaagtgg aatccgccgg
    22381 gagcgttccg cgccgccatc tcttctatca gcccggacac aaactggcgc gcgaggggcc
    22441 gcccctccaa cgaaccgggg agattgaaga tcacggcttc ggggaattcg ccaagaatcg
    22501 ccgacattac tacgcgcccc tgcctgtacg aagcctccat caagtcaccg ggtgtatagt
    22561 cagagtacgt gtaaaccggg tggtcaagcg agtaacgcgg gtacgggtat tcggtgtcaa
    22621 tgctgattcc acggaagcca gtcgccttcg cggctttgcc cagcgctgaa aaatgttgtc
    22681 tcaacttttc ggtgaactca ccagaaagga gggtctccgg tgaaggccac ggttcgctgt
    22741 caccaaagga aatcgcgagc aggttttcgg cgcagccaac cgatttgagc gcggcgacat
    22801 tctgtccggc cagcctcagc cagttcgggt tggtcgcagc ggtgttgccg acgccgatgt
    22861 tcgtcagcac acccgaatcc cagctgccac actggaaaaa gaacccgtcg tagagcccct
    22921 tgacgttggc cgcatgttcg ttgaggtagg gcgtgggtga caccgagtaa cagatgatcg
    22981 cgggtatagc gagagcagtg gagcgggcat ggagccttcc tttctctttc tttccggttc
    23041 cggattgtgc cgtgggagtt ctgatgtgga aacctgcgcg tggagtccga tcgtaacaag
    23101 gcaggaaact gccatgggat gattgccgaa atcttcgagg ttttccgtta accaaagtat
    23161 gaccgaattt cccgctgcgc aacgggtggc ctagcgtcgg cacggagact ccgcccgcgt
    23221 ttgccaatga atcggaggaa ctcaactgca acatccgggg ttattcatgg agagcggggt
    23281 gggtatcccg gagtgggtac cgcatggctc ccccaacgcg ccacaccgag cttacttttc
    23341 gaacattttg cttgcaaaag tcggaaggcg ccggtacatt ttactgtcta cacactttgc
    23401 gagcgcatcg gcacgaacac gcgacggaga ctgggaacca tggtcaaaac ccccatatac
    23461 atggacaatc acgcgacgac accggtagac ccgcgtgtgc ttgaggcaat gttgccctat
    23521 tttaccgagg ttttcggcaa tgcggcgagc agaaaccatg tgttcggtca tgaagcggaa
    23581 gctgccgtag agcaaatgcg aggagttatc ggcgcagccg tcggcgcgga acccagaaac
    23641 gtcattttca ctagtggcgc gacggaatcc gacaatatgg ccataaaagg ggttgcggaa
    23701 ttctacaggg aacgcggtaa tcacgtcatc actcaggtga ccgagcacaa agcggttctc
    23761 gacacctgca aggaactcga gcgacgcggt tacgaagtaa cctacctgcc ggttgatcgt
    23821 tacgggctta tcgacctcga cgacttgcgg aaagcaatca agtcggaaac gatactgatt
    23881 tccataatga tggccaacaa cgaaatcggc acgattcagc ctgtcaaaga aatcggcgcc
    23941 atcgccaagg aaaagggggt tttgttccac agtgacgcga cgcaggcggt tgggaagctg
    24001 cctgttgacg tagacgaact cgggatagat ctcctttcga tgagcgccca taagatttac
    24061 ggccccaaag gcgtgggagc attgtacgtt cgaggaaaga acccgcgtgt ccggcttgct
    24121 cctctcttcc acggaggagg gcatgaacga gggatgcgtt ccggaacgct caacgtgtca
    24181 gggattgtag gtttcggcaa ggcgttgcag atcgccgttg acgagatgga aagtgaatcg
    24241 gcacgcgtga
//
LOCUS       DAOSOO010000026        23545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2176_length_23545_cov_5.764240, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000026 DAOSOO010000000
VERSION     DAOSOO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 23545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23545
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_23545_cov_5.764240"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLT86_03360"
     CDS             <1..332
                     /locus_tag="PLT86_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT35685.1"
                     /translation="LDAKITEQVTGTAEIRQVFKVPRVGNVAGCMVRSGVMTRGARVR
                     VMRDGAEAYNGKIGTLRRVKEDVREVPSGYECGISIENFNDIKVGDIIEAYTIIEEAR
                     TLESVAG"
     gene            345..626
                     /locus_tag="PLT86_03365"
     CDS             345..626
                     /locus_tag="PLT86_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOT35686.1"
                     /translation="MVVASVCIGCLVRDSSSLKDKRQVIRSLKDRIRSHFNVSVAEVD
                     HQDLWQRATIGVSVVAVDAQSARHVLEQVIRFVEHDVRLSLLDAPVVIH"
     gene            653..1039
                     /gene="rbfA"
                     /locus_tag="PLT86_03370"
     CDS             653..1039
                     /gene="rbfA"
                     /locus_tag="PLT86_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT35687.1"
                     /translation="MPFRAEQVADQIRRELAEIIRLRVSDPRVGPITITRVKVSDDLS
                     FARVFFAELTVEGRREGDTLKGLASATGFVRTELGKRIGLRHVPELRFQIDHSIEDGI
                     HLSSLIEKVRTEDKRKLPPEAPGSDV"
     gene            1032..1931
                     /gene="truB"
                     /locus_tag="PLT86_03375"
     CDS             1032..1931
                     /gene="truB"
                     /locus_tag="PLT86_03375"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT35688.1"
                     /translation="MCSERALDGIVLLDKPAGGTSHGAVAHLRRILRTRHIGHGGTLD
                     PAATGVLVILVGRATRLAGFLGGGTKVYEATFRFGTATTTDDAAGEVIETADVPELDF
                     GGIEASKNSFVGEIHQVPPAFSAVRSDGVRAYRRARRGQAMELAPRKVTISEIEILEW
                     VRPDLRLKVSCTSGTYIRALARDWGRAIGSAAHVRSLRRVKSGLFDIAECLSFEQIEQ
                     LAASGAWEHFIVPPGPALEKAIGRTTIVEGQDEKRFLNGAPIAHFDDFDGISVVFSRE
                     RELLGIGRVCAGVLRPVLVYPSL"
     gene            2044..2313
                     /gene="rpsO"
                     /locus_tag="PLT86_03380"
     CDS             2044..2313
                     /gene="rpsO"
                     /locus_tag="PLT86_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT35689.1"
                     /translation="MPVSKERVAEVMAQHRHHEADTGSSEVQIALLTERIRNLTEHFK
                     THEKDHAGRRGLLKLVGQRRRLLNYLERTNIESYRKLIAELDLRR"
     gene            2553..4706
                     /locus_tag="PLT86_03385"
     CDS             2553..4706
                     /locus_tag="PLT86_03385"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT35690.1"
                     /translation="MVREYSVAVGDRTITVEHGRMAKQADGAVLVRCGDSMVLVTACM
                     SPQEMDLGFFPLTVEYREKGYAAGKIPGSIFKREGRPSDQETLSARLIDHQIRPLFPK
                     SLKNEIQVIVSVLSHDQENDPDIIACIGASLALSISSIPFAGPYATVRVAHDQEGNKI
                     VLPTYAQLEESDADVVVAGNTESVINIEGETHELPEAALLDLMEFAHGHIKDLCAFQQ
                     KIVDEIGKPKVVPVDTPLDERLVARVEGLSVARICESLRVADKVAREEAQAAIAAEVN
                     TALAEEFPGQEKGIASVLEKIAKREMRNMILNEGRRIDGRTIDEVREITSEIGVLPRA
                     HGSALFTRGQTQALASVTLGTKMDERLVDSLEGKSFKTYMLDYNFPPFATGEVKRMSG
                     PGRREIGHGFLAERAIEPVIPASESFPYTIRIVSDILESNGSSSMATVCAASLALMDA
                     GVPIKCAVAGVNCGLVSDGDRFAILTDLLGVEDANGDMDLKTAGTEQGITAFQMDIKV
                     GGIPMETMKLALERAREGRLHVLGLMKQAIAEPKPELSRFAPRILVVKVDPSQIGSII
                     GPGGKTVREIQELTGATIEIDDDGTVRVSSLDGESGQAAVRRINGIVRKPVVGEVFDG
                     VVKSILDFGAVVEYLPGKEGLLHISEIANRRLEKVEDEIKVGDPVQVKIIEVDNQTGK
                     VRLSHKRLLPDYDPSKERERPPRSSQSGHSHGRGR"
     gene            4708..5160
                     /gene="dut"
                     /locus_tag="PLT86_03390"
     CDS             4708..5160
                     /gene="dut"
                     /locus_tag="PLT86_03390"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT35691.1"
                     /translation="MVDRLPIRIVRLPHGKRFPLPAYQTDGAVGMDLVAAIESPLTVP
                     SGHTVRVPSGIAIAIPEGYEGQVRPRSGLASRLPLIIPNSPGTIDSDYRGEVQILLTN
                     IGREAIEISPGTRIAQLVIAPVVRAEWCEVEELPPTGRASGGFGHTGT"
     gene            5170..5967
                     /locus_tag="PLT86_03395"
     CDS             5170..5967
                     /locus_tag="PLT86_03395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT35692.1"
                     /translation="MAQHIFAYHAVEDGFELSATTVPRRRWREHCRVLSDLGWRAVRV
                     DEMIQGADDGTFCITVDDAYASLLDVVATCECHGWRGTVFVPTGYVGKVASWDVGAWR
                     THRHLNWEELREVAAAGWEIGVHGHTHTALTDKPIAAARQELQCARSAMEQELGIVAT
                     SLAYPFGAASKTLAEMAQAIGFRRAVTMEPSQVEEWHSPLLLPRWPVYRMDTAAHLQA
                     RLRGAPWLKELERARLRTIQRFSQGTRVRMSGKRNIISDAAEQTYVE"
     gene            5957..7261
                     /gene="asnS"
                     /locus_tag="PLT86_03400"
     CDS             5957..7261
                     /gene="asnS"
                     /locus_tag="PLT86_03400"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT35693.1"
                     /translation="MWNERPTIEEIARYEGQEIVLKGWLNNMRSSGKLHFLQVRDGTG
                     VMQCVVFKGNVTPEVFETAAALKQESSLVVRGQIRADARSPIGFEMDVVDLQAIHVPE
                     VDYPISPKEHGTAFLMDHRHLWLRSSRQQAILRIRAELIRQIRAYFDANGFLLLDAPI
                     FTPSACEGTTTLFETDYVDGSKAYLTQSGQLYMEAGATAFGRVYCFGPTFRAEKSKTR
                     RHLTEFWMVEPEVAHIGLDEDMDLAEDFLVSIVQGVVANRKKELTALERDIAALERVQ
                     KPFARISYTEAVERLQQAGMPVRWGEDFGGDEETVLANQFDRPVMIHRYPAECKAFYM
                     KTDPDDPKCALCVDVLAPEGYGEIIGGGQREDDYDTLLQKIHTHGLSEEAFGWYLDLR
                     RYGTVPHAGFGLGVERTLGWICGLPHVRETIPFPRLMQRVTP"
     gene            7307..9766
                     /gene="leuS"
                     /locus_tag="PLT86_03405"
     CDS             7307..9766
                     /gene="leuS"
                     /locus_tag="PLT86_03405"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT35694.1"
                     /translation="MIPYDFRTLEPQWRAWWEENKINEAKDDSSRPKYYCLDMFPYPS
                     GEGLHVGHWRNYVLSDVWSRYQRLRGYQVLHPIGWDAFGLPAENFAIKHKIHPRISTA
                     RNIANMKRQLKIIGAMYDWSREISTCDPEYYRWTQWIFLRMYKRGLAYRSTAPVNWCP
                     SCRVGLANEEVVAGGCERCGTAVVKRDLVQWYLRITAYAERLLRDLERLDWPERVKTM
                     QANWIGRSEGARITFTAVHPGNQTTYPIDVFTTRPDTLFGATYMVLAPEHPLVPALTM
                     ASRRKEVEAYREQARNVRDVDRTAATRPKTGVPLGSVAINPANGNRIPIWISDYVLMG
                     YGTGAIMAVPAHDERDFEFARTFNLPVVEVVYHHETVHDANGRIQAAYTGEGTMIQSS
                     GFDGLDSRTGGRQIVEMLARQGKAEATVSYRLRDWLFSRQRYWGEPIPIVYCEKCGEQ
                     PVPEEQLPVLLPEVESYEPTGTGESPLAGITEWVNTVCPVCGGPAKRETDTMPQWAGS
                     SWYFLRYASPDEKAAAFDREKVNKWLPVDMYVGGVEHAILHLLYARFFTKVLFDEGAI
                     SFDEPFTKLFNQGMICRKSEKTGRVEKMSKSKGNIVSPDGLVEKYGTDAVRMYELFVG
                     PPEQDAEWNDNGIEGISRFLHRAWGLITSNLEPSANPERLLRARHRLVKQVTEGLESF
                     KFNTTISRMMEFVNFALDPEGGNGKLDAGTRDAFIVLLAPFAPHMAEELWKLAGKQNS
                     VFDTTVWPSFDEGLASAEKMTIAVQVNGKIRATVELDADSSDETARAEALAQPNVQRY
                     LAGKEPRKIIVVPGRLVSIVV"
     gene            9877..10416
                     /locus_tag="PLT86_03410"
     CDS             9877..10416
                     /locus_tag="PLT86_03410"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOT35695.1"
                     /translation="MPSKIVLTKGVGRHREQLTSFEMALRDAGLAAFNLVPVSSIFPP
                     RCKLVSKRAGLAELHPGQVVFTVMSRAASNEPHRLLAATIGLAIPRDRNKYGYLSEHH
                     SFGQTEEVAGEYAEDLAAEMLATVLGAEFDVNQSWDEKRELWRIQNEIVATRSISQSA
                     MGHKDGLWTSVLAAAVLIP"
     gene            10580..11479
                     /gene="speB"
                     /locus_tag="PLT86_03415"
     CDS             10580..11479
                     /gene="speB"
                     /locus_tag="PLT86_03415"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOT35696.1"
                     /translation="MNLSQFPRNLQDVPFTFLGLPDPEGGLGEARVCVLPIPYESTTT
                     YRGGTRSGPAAIVDASRYVETFDEEMLWDLADMPILTLPSVSPLLGAAEAMSERIRDV
                     AREVVSRDRLLISLGGEHAITAPLVEAHSEEFGHLTVVHLDAHADLRESYEGSRMSHA
                     SVIKRVAEHTDVLSVGVRSISSEEMDWLRNQERVRVVFAHEIRKDQGLLERVLGELTG
                     NVYLTIDADVLDPGVMPGVGTPEPGGLSYDQVVHVIKRLSVGCSIVGADLVELAPVPG
                     NVVSEFTAARIVAKIVAYTKNPV"
     gene            11493..11969
                     /locus_tag="PLT86_03420"
     CDS             11493..11969
                     /locus_tag="PLT86_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT35697.1"
                     /translation="MAKPNDDTGKTSETVGYPPEKLKEFEAWLHQARQRILKGYDDQK
                     AEIDGFKGDTESDAYDDASNIVEMNILMALNDTQKKELSQIGLALLKVKDGTYGICEG
                     CGELIGEQRLKFLPFANLCVKCKDKEERGTVVIQRDTHIVPPDEDFLVLTDSDEDY"
     gene            12047..12361
                     /locus_tag="PLT86_03425"
     CDS             12047..12361
                     /locus_tag="PLT86_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35698.1"
                     /translation="MAKSRIETFKAFLEKDPGNSLARYSLAMELRKVGRYDEALAGFA
                     DLIERDPKYVPAYFMCGQTAFEAGKVQEAKEVLRAGIDVARNAGETHAAEKMAELLAT
                     LE"
     gene            complement(12437..13441)
                     /locus_tag="PLT86_03430"
     CDS             complement(12437..13441)
                     /locus_tag="PLT86_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35699.1"
                     /translation="MSEKIVRAAIIGYGGAFNMGAHHTREMHATGRMKLVAVCDIDPA
                     RTEQAKKDWPGIQTFNDVDELLAKGNFDLAVVILPHNQHAPVAVKCSNAGKHVIVEKP
                     MCLTVKEANDMINAAKKARRMLTVYHNRRHDGDFLTLKSLVQRGVIGKVFSVEMGGMG
                     WGHPGTWWRSKKDISGGAFYDWGAHFLDWLLQIVPNKIENVTGFFQQDLVWKDVTNED
                     HVHAVIRFDENVVADVQMSHIARVGRPRWKLLGSKGAIVSEKGGFRVYGEFDGVLAEG
                     FVSDQPGTHPKYYENIAAHILDGAELDVKPEQARRVIAIMETAEISSKSGVSEKMPVD
                     "
     gene            complement(13582..14784)
                     /gene="argJ"
                     /locus_tag="PLT86_03435"
     CDS             complement(13582..14784)
                     /gene="argJ"
                     /locus_tag="PLT86_03435"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT35700.1"
                     /translation="MERTRTGVCAPKGFSATGVACGLKAHGRRDLALIVSRREASAAA
                     LFTTNLVCGAPISVSRQHITGGVARAIVVNAGCANACTGARGVADAERMCSLVASGLN
                     CPPRQVLVASTGVIGRLLPMDRIERGIEAALDDVSEDGGSAAADAIITTDTFPKTVKR
                     VLSIGGHTVTIGGMAKGAGMIAPKLATMLAFITTDALLAPEDAHSLLRNAAEASFNRL
                     TIDGDTSTSDTLTLLANGESGAPALKPGTDYYVRFGEQLTDACRDLAKMIARDGEGAT
                     KFITVHVSGAPSNQDAHVAARTVAESPLVKTAFFGGDPNWGRIAAALGRSGVFFNPGR
                     LTIRVDDLLLFENGMPAAFPLAAAEEKFREKVITIHADLGDGQGEAEVYTCDLSYEYV
                     RINAEYTT"
     gene            14974..15426
                     /locus_tag="PLT86_03440"
     CDS             14974..15426
                     /locus_tag="PLT86_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35701.1"
                     /translation="MRADLESETRQADEPFLARFDPKPDESALWTLVSGIGQFVVAGW
                     CITIAGLMIVVGYAGMHGRGSVLASAAAILMTLFVLVGVALAAIGVRFLARSARAFWV
                     AMRKALFVVSFNQAADKRRALHAIVGDPLLEPYARYLLQRGDLKVPPL"
     gene            15788..17320
                     /locus_tag="PLT86_03445"
     CDS             15788..17320
                     /locus_tag="PLT86_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT35702.1"
                     /translation="MASRTVILINPDREKPLIAPLGLDYLAATLRHAGYEPELLDLAF
                     ESDWYAATNQAVSKRDLLAVLILIRHLDDGLYNSQTFFLPKSRTLIEYLRSKTKAPIG
                     VIGGAYSLVPEAILRFCRADFGIANDPEVALPMLLEAIESGDGYEGVPNVGWIEGKAF
                     RRNPIKTARVDGEWLAERSLIDNVRYFNESGVTRFETKRGCAHNEVYSTDPITRGNSV
                     RLRPAEHVAEEVERLAARGIYSLYADDTRFNDPREHALEVCRAMAERKLRDRVRWYAT
                     CTPKGLDAELAEAMEAAGCQGVELFTDSGDADQLRRMKHTHTPEDILTAIESCKRYGI
                     VVISEVQIGGPGESRKSIERTLGLMKASGPDMINIHYGLRVYPNTPLGNAVQTVTPLR
                     NNFHLRGAIYNNESLLRPVFYIESLLGRGVEEFIEELIDHDPRYLFAFRKEVDKTYNY
                     NDPTILTEAILHEGHRGAQWDIWRRLIYRLPPLSLNGDRPSRGKRRPRRVIQSAEAGV
                     AV"
     gene            17412..18185
                     /locus_tag="PLT86_03450"
     CDS             17412..18185
                     /locus_tag="PLT86_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35703.1"
                     /translation="MKLGTCAYSYRDLLTKGKMTLIELLDTVRDLGFDGIEWTAYYFP
                     ETTKEYLLHIKRETFVRGLDISATAVGGNFIQPTREEREKQIANVKSWIEKSAWMGST
                     ALRVFAGYVTEGMAREDADQMVRDGLAECARLAEKEGVILALENHGGVTADAEGTLRL
                     VKPLNNPWVKLNLDFGNFTGDIYAQYEACAPYAVTTHTKVTVRQGEAREKVDYRKVVR
                     IMRNAGYRGYINIEFEEPEDPIVGVDRFAAYLRGCMEDA"
     gene            18265..19290
                     /locus_tag="PLT86_03455"
     CDS             18265..19290
                     /locus_tag="PLT86_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT35704.1"
                     /translation="MKRPFALFCTALLLVVSGCLGTIPLGERGRAAHVLRFDDNGVFT
                     IVQFTDTHLGSRDAGLRDDSTYALLDSIIRWENPDLIVLTGDIVRFAWEPQAVPSWRR
                     LIAFFDSTGVPWAFVHGNHDAEVRGYAAVDSLIATARNCLYTSGPDSLNQPGNYVLPV
                     LSRKGRGVSALLWCMNSGAGSSEPSGYGWIRANQVKWFRATSERLKTQTQPNLTQLAF
                     FHIPFAQYQTVWDARTCTGYKNEKVCMQGKDEGAFAAFVEYRVNGCFVGHDHTNDYEG
                     TLEDVTLCYGRASGYRGYGKEGFLRGARVIKIKDGVRGFTSHIRLADGSLAERPVHTP
                     GTALPTP"
     gene            19438..20313
                     /locus_tag="PLT86_03460"
     CDS             19438..20313
                     /locus_tag="PLT86_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35705.1"
                     /translation="MAKQTDMYDPTLKDQSEYDHKEIYIISNTHWDREWVYPSAETRL
                     LMLEFMDNMLDLLDKQPDYHSFTMDSQTLCIQDYLDFRPERREQIERHVKSGRLIIGP
                     WCSLPEEYIVNGESLVRNLVIGHKVANSLGKVSKVGYTPFSYGQTSQMPQIYRGFDID
                     TIIFYRGIKTEKSEFIMRGPDGSELLGMRFGALSRFSYFFYLFRMVAYNMSRDEAKYD
                     WFRGSLPFKLAGDHHVTRITTYAIRMPRAGIPSTLIQAQGNSCATNPSISARGISRPC
                     RVLIVPNPIPASSTL"
     gene            20373..22301
                     /locus_tag="PLT86_03465"
     CDS             20373..22301
                     /locus_tag="PLT86_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT35706.1"
                     /translation="MDRLKEEVAAGRLKPEVIEGESRNPNSVGKWTHLFGDVISSRVR
                     IKLMNNANEQNVQRKAEPFCALAMTLGAKYPTTVLDECWDNIMKNHPHDTICGAGVDQ
                     MEKDMIARGEQVNIFSEGLYRRALQKIQTRIDNSDVAANEIALTVFNPSPFPRSEVVS
                     VLLDLPDVCKYEDFTIRDEKGNKVEAQYAEFYPWGTLVRNLRDVSLQLHAERVRVHFE
                     ALDVPPFGYRTYTVKHEEDVVPKPMKTLVTAVNTMENEHLKVKINANGTLQITNKETG
                     DVFDNMHYLEDGGENGHSWTTIPPDHDEIITSHGANVYIAQMESGPALARFRVEYHMM
                     IPEALDFETMEAYTGQTPRAQIRHPRISKRSERRKEMVIRSEFTLRKGSKVLEVYTKV
                     NNECENHRLRVCFPTGIQAATSDSEASFDVIQRQVIRTPDNWYWGRPNPVLPMHRFVS
                     FSNGKSGITFLNNGIREYEAMEDADRTVALTLFRAFTFRNPPILDTYDVYPEMKLSQC
                     PGEMEFRYNIYPHKGDWAAAEVYKEADHINLPLEVAEVGPHKGDLPKSMSFLSISPAN
                     LVLSAFKKAEERDTFILRVFNPTGDAMNGTITFYRPIKKAWLCNMNEERRQDLEPKGN
                     VLTLDVPKKKIMTIEFEA"
     gene            22605..22970
                     /locus_tag="PLT86_03470"
     CDS             22605..22970
                     /locus_tag="PLT86_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT35707.1"
                     /translation="MAKAISLAPVIIAIILAAFGQVTMKYAMFGSPLPVTSPVEAARN
                     ILSRPMVYVGLVFYAGSSFLWLISLSRLDLSYMYPFTALLIVIITFVSALVFNEPITL
                     WKVVGVFLICAGLLSIARS"
     gene            23135..>23545
                     /locus_tag="PLT86_03475"
     CDS             23135..>23545
                     /locus_tag="PLT86_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT35708.1"
                     /translation="MNALVKYLLAFVLIFCGFQIASAEAPPTDTLRLTLRECLDRAMQ
                     GNLDIREAQLERDRALIDQLEANRAFWLPVFEARSYLSVVKDARGYVTDHPLTTSWDN
                     FGPCFEFHLQAAQPISTLGRVNALKRAARNGIAAR"
BASE COUNT         5188 a         6418 c         7110 g         4829 t
ORIGIN      
        1 ttctggatgc caagatcacc gagcaggtta ccgggaccgc agagatcagg caggtgttca
       61 aagttccacg cgtgggcaac gttgcgggtt gtatggtccg gagcggtgtc atgacacgcg
      121 gcgcacgtgt gcgggtcatg cgggatggtg cggaggcgta caacggtaaa atcggaacac
      181 tccggcgcgt caaagaggac gtccgggagg ttccctccgg gtacgaatgt ggtatctcga
      241 ttgagaactt caacgacatc aaagtgggcg acattattga agcgtacacc ataatagaag
      301 aagctcgcac cctggagtcc gtcgcaggat gatcggggaa accgatggtg gtcgccagtg
      361 tgtgtatcgg gtgtctggtt cgcgacagca gctcgctcaa ggacaagcgt caggtaatca
      421 ggagtctcaa ggatcgaata cggtcccatt tcaatgtctc ggttgcggaa gttgaccacc
      481 aggacctgtg gcagcgggca accatcgggg tttcggtcgt tgccgttgat gcccagtccg
      541 cgcgccatgt tctcgaacag gtgatacggt tcgtggaaca cgacgtgcgg ttatcgttgt
      601 tggatgcgcc ggtggtcatc cactgatcat cagatcttat gaacggggta ccatgccttt
      661 tcgggcggaa caggttgccg atcagatccg gagggagctc gcggagatca ttcgtctccg
      721 cgtgagcgac ccgcgggtag gcccgattac catcacgagg gtgaaagtct ccgatgatct
      781 ttcctttgcc cgcgtctttt tcgcggagtt gaccgtcgaa gggcgcaggg aaggcgatac
      841 tctcaaaggc ctcgccagtg cgaccgggtt cgtacgaacg gaactcggga agcgtattgg
      901 gttgcggcat gtccccgaac tgaggtttca gatagaccac tccatcgaag acggcataca
      961 tctgagcagt ctgatcgaaa aggtgcgaac ggaggacaag cgcaaactgc caccggaggc
     1021 gccaggttca gatgtgtagc gagcgggctc tggacgggat tgtcctgctc gacaaacccg
     1081 ccggcggcac gtcgcacggt gcggtggcgc atctgagaag aattttgcgc acccgtcaca
     1141 tcggccacgg aggcacgctg gaccccgcgg caacgggcgt gctggtgatt ctggttgggc
     1201 gcgcgactcg gctggcgggg ttcctgggcg gcggaacgaa ggtgtacgaa gcgacgttcc
     1261 gcttcggaac cgcgacaacg acagacgacg cggcgggaga ggtgatcgag accgcggacg
     1321 ttcccgaact ggattttggg ggtatcgaag cctcgaagaa cagctttgtc ggcgaaatcc
     1381 accaggtgcc accagccttc agcgcagttc ggtcggatgg ggttcgagcc taccggcggg
     1441 cacgccgcgg acaggcaatg gaactggccc cgcggaaggt cacgatttcg gagatcgaaa
     1501 tcctggaatg ggtacgcccg gacctccgtc tgaaggtgtc gtgcaccagt ggaacgtaca
     1561 tccgcgcatt ggcgcgtgat tggggaaggg cgatcggaag cgcggcgcat gtgcgatcgt
     1621 tgcggagggt aaaatcaggc ttatttgata ttgcagaatg cctgtctttt gagcagattg
     1681 agcaattggc ggcgtcgggc gcgtgggagc atttcatcgt gccgccgggg cccgcgctgg
     1741 agaaggcgat cgggcgcacg acgatcgttg aagggcaaga tgagaaacgt tttctcaacg
     1801 gggcgccgat cgctcacttc gatgactttg acggaatttc ggttgtgttt tcgcgggaac
     1861 gcgaattact gggcatagga agagtgtgtg ccggcgtatt gcggccggtg ttggtgtacc
     1921 cctcactgta aagcggaaag gccgccagcg ggatgggaaa gacgtttcgc cctgcctcca
     1981 gacaccggag gctgtgtggt gcggagccgc aagactccgc atccggtgag aaaggagatt
     2041 caggtgcctg tttccaaaga gcgtgtcgca gaagtcatgg ctcaacatcg ccatcacgag
     2101 gctgataccg gatcgtcaga agtacagatc gcgctgctca cagagcggat tcggaacctt
     2161 accgagcact tcaagacaca cgagaaagac catgccggcc gccgcgggct gctcaagctt
     2221 gttggccagc gccgccgatt gttgaattat ctcgagcgga cgaatattga gagctatcgc
     2281 aagcttatcg cagagctcga tttgcgtcgt taggcatggt accccacccc gagggccctg
     2341 tgagaatccg cggggtgagg ggtttgccgg catgtccggc ccggcgtcac tcccgacgtg
     2401 tcccgaaccg ggatcacgtt caaggcttcg atgtacgcgc aggcgcgtca ccgtcacccg
     2461 catgggtgcg aaaaagacca gaacgcgctt acgccctgag cccggcgtcg gtcacaggtt
     2521 gaattcacgc gttcccacga aggaggacct gaatggtccg tgagtacagt gttgcagtcg
     2581 gagatcgcac gataaccgtg gaacacgggc gtatggcgaa acaggcggac ggggctgtcc
     2641 ttgtccggtg cggcgactcc atggtcctgg taaccgcttg catgtctccg caggagatgg
     2701 atcttggttt tttccctttg acggtggaat accgcgagaa agggtacgcc gcggggaaaa
     2761 tcccgggaag catcttcaaa cgtgaaggaa ggccgtccga tcaggagaca ctgtccgcgc
     2821 ggctcattga ccaccagatt cggccgctgt ttccgaagag cctgaaaaac gagatacagg
     2881 tgatcgtgtc ggtgctttct cacgatcagg aaaatgaccc cgacattatc gcctgcatcg
     2941 gcgcgtctct ggctctcagc atctcctcca tcccgtttgc gggtccgtac gcaacggttc
     3001 gcgtagcgca cgaccaggaa ggcaacaaaa tcgttcttcc cacctatgcg cagttggagg
     3061 agtcggacgc agatgtcgtc gtcgcgggca atacggaatc ggtcatcaat atcgaaggcg
     3121 agacgcacga attgcccgaa gcagcgttgc tcgacctgat ggagttcgcc cacggccaca
     3181 tcaaggatct ttgcgcattc cagcagaaga tcgtggatga gatcggcaaa ccgaaagtcg
     3241 tccccgtgga tacacctctg gatgaacggc tggtggcgcg agtggaaggg ctctccgtag
     3301 cgcgcatctg cgaaagcctg agggtcgccg acaaagttgc gcgggaagag gcgcaggctg
     3361 cgatcgctgc ggaagtcaac actgcgctcg cggaggaatt tccgggtcag gaaaagggaa
     3421 tcgcatcggt cctggagaag attgccaaac gggaaatgcg gaatatgatc ttgaacgaag
     3481 gtcggcgaat cgacggccgc accatcgacg aggtcaggga gatcacttca gaaatcggcg
     3541 tcctgccgcg tgctcacggt tccgctttgt tcactcgcgg tcagacgcag gctctcgcgt
     3601 ccgtaacgct cgggacgaag atggacgaac ggctggtcga ttcccttgaa gggaaatcct
     3661 tcaaaacgta catgctggat tacaatttcc cgccgttcgc aaccggcgaa gtgaaacgca
     3721 tgtccgggcc gggaagacgc gaaatagggc atggattcct cgccgaacga gccatcgagc
     3781 ctgttattcc tgcgagtgag agtttcccgt acacgattcg tatcgtcagt gatattctcg
     3841 aatcgaacgg gtcctcttca atggcgacgg tctgtgccgc gagtctggcg ctgatggacg
     3901 caggtgtccc gatcaaatgc gcagttgccg gggtgaactg cgggctggtt tcagatggcg
     3961 accgttttgc aattctgacc gacctgctcg gagttgaaga cgcgaacggc gacatggacc
     4021 tgaagaccgc agggacggag cagggcatca ccgccttcca gatggatatc aaggttggtg
     4081 gaattcccat ggaaaccatg aaactggcac tggagcgcgc aagagaaggg cgtctgcatg
     4141 tgctgggcct catgaagcag gcgattgcgg aacccaaacc cgaactctcc cggtttgctc
     4201 cgcgcatcct cgtggtgaaa gtggatcctt cgcaaatagg ttcgatcatt ggccccggtg
     4261 ggaaaacggt tcgtgagatc caggagctca ccggcgcgac catcgaaatc gatgatgacg
     4321 gcactgtgcg ggtctcctcc ctcgacggag aaagcggtca ggcggcagtt cgaaggatca
     4381 acgggattgt gcggaaaccg gtggttggcg aggtttttga cggcgtcgtc aagagcattc
     4441 tcgatttcgg cgcggtcgtc gagtatctgc ccggcaaaga aggcctgctg cacatctccg
     4501 aaatcgccaa ccgtcggctc gagaaagtgg aagacgaaat caaagtcggc gatcccgtgc
     4561 aggtgaaaat catcgaggtt gacaatcaga cggggaaggt tcgcctgagc cacaaacgat
     4621 tgctgcccga ttacgatccg tcaaaggaac gcgaacggcc gccacggagt tcgcaatcgg
     4681 ggcattccca cgggcgcggg cgctagaatg gttgatcggc ttccgattcg catcgtccgt
     4741 cttccacacg gaaagaggtt tcctctgccg gcgtaccaga cggatggcgc agttgggatg
     4801 gacctggtgg cagctataga gtctccattg acggtccctt cagggcacac cgtcagggtg
     4861 ccttccggaa ttgcgattgc cattccggaa gggtacgagg gtcaggttcg tccgagaagc
     4921 gggttggcaa gccgtttgcc gttgatcatt cccaacagcc cggggacaat tgacagcgac
     4981 tatcgcggag aggtgcaaat cctcctcacg aacatcggcc gggaggcaat agagatctca
     5041 cctggaaccc gtattgccca gctggtaatt gcgccggttg ttcgcgcgga atggtgcgaa
     5101 gtcgaggaac ttccgccgac cggacgtgcc tccggtggat tcgggcacac gggcacatag
     5161 gcggcctcga tggcgcagca catcttcgcg taccacgccg tggaagatgg gttcgaactg
     5221 agcgccacga cggttcctcg gcgacggtgg cgcgaacatt gcagggttct gtcggatctt
     5281 ggttggcgtg ccgttcgggt ggatgaaatg atccagggag cggatgacgg cacgttctgc
     5341 atcacagtgg acgatgcgta tgcgagcctt ttggacgtgg tcgcgacgtg cgaatgccat
     5401 ggttggcgtg ggaccgtgtt tgtgccgacc ggctacgtag gaaaagtcgc gtcgtgggat
     5461 gttggcgcgt ggcgcacgca caggcatctc aactgggaag aactgcggga agtagcggca
     5521 gccgggtggg agattggcgt acacggtcac acccatacgg cgcttactga caagccgatt
     5581 gccgctgcga ggcaagagct gcaatgcgct cgttcggcaa tggaacagga actgggcata
     5641 gtggcaacga gcctggccta cccgtttggt gcggcgtcaa agacgctggc agagatggcg
     5701 caggcgattg gttttcggcg cgcggtgacc atggagccgt ctcaagttga agaatggcat
     5761 tccccgttgc tcctgccgcg gtggccggtg taccgcatgg acaccgcagc acacctccag
     5821 gcacggttgc gcggagcgcc atggttgaaa gagttggagc gggcgaggtt gcggactatc
     5881 cagcgattct cgcagggaac gcgtgtacgc atgtcgggga agagaaacat catatctgac
     5941 gccgcggagc agacctatgt ggaatgagcg gccaaccatc gaagaaatcg cccggtacga
     6001 aggacaggag atcgtcctca aggggtggtt gaacaatatg cggtcgtcgg gcaagctgca
     6061 ttttcttcag gtgcgtgacg ggaccggcgt catgcagtgt gtggtgttca aagggaacgt
     6121 cacgcctgag gtcttcgaaa cggcggcggc tctcaaacag gagtcctcgc tggttgtcag
     6181 agggcagatt cgggccgatg ctcgctctcc catcgggttt gagatggacg tggtcgatct
     6241 gcaagccatt cacgtacccg aagtggacta cccgatttcc ccgaaagagc acggcactgc
     6301 gttcctcatg gatcaccgtc acctgtggct cagatcctcg cggcagcagg cgattttgag
     6361 gatcagggcg gaactcatca ggcagattcg cgcgtatttc gacgcaaatg gttttctgtt
     6421 gttggatgcc ccgatattca cgccgtctgc ctgcgaagga acgacgactc tttttgagac
     6481 agattacgtt gatgggagca aggcgtacct cacgcagtcc gggcagctct atatggaggc
     6541 cggagcaact gcattcgggc gcgtgtactg cttcggaccg accttccgtg cggagaagtc
     6601 aaaaacacgc cggcatctca ccgagttctg gatggtggaa ccggaagtgg cgcacatagg
     6661 gcttgacgaa gatatggacc tggcagagga ctttctggtg tccatcgtgc aaggggttgt
     6721 cgcgaaccgg aagaaggaac tcaccgcact ggaacgcgat atcgccgcgc tggagcgggt
     6781 gcagaagccc tttgcccgaa tcagttacac ggaggctgta gaacgcctgc aacaggcggg
     6841 gatgccggtg cgctgggggg aagacttcgg cggcgatgag gagaccgtgc ttgcgaacca
     6901 gttcgaccgg ccggtgatga tccaccgtta ccccgccgag tgcaaagcct tttacatgaa
     6961 aacggatccg gatgacccga agtgcgctct ttgtgtcgac gtactggcgc cggaagggta
     7021 cggggagatt atcggtggcg ggcagcgcga agatgactat gatacgctcc tgcagaaaat
     7081 ccacactcac gggctttccg aagaagcatt tggctggtat ctcgatttgc gccgctacgg
     7141 aacggtgccg cacgcaggat tcgggctggg tgtggaacga acactcggtt ggatctgcgg
     7201 attgccgcac gtgagggaaa cgataccatt cccgcggttg atgcaacgag ttacgccctg
     7261 acacaacgaa gaaccaaaaa gagcgaatct ggagaaggca tttcgcatga ttccctatga
     7321 tttccgcacg ctggaaccgc agtggagagc gtggtgggaa gagaacaaga tcaacgaggc
     7381 gaaggacgat tcctcacgcc cgaagtacta ctgtcttgac atgttcccgt atccctccgg
     7441 cgaagggttg cacgtcgggc actggcgcaa ttacgtgctc agcgatgtgt ggtcacggta
     7501 ccagaggctc cgcggctacc aggtccttca tcccatagga tgggatgcat ttggtcttcc
     7561 cgcggagaac ttcgcgatca aacacaagat tcacccgcgg ataagcaccg cacggaacat
     7621 cgcgaatatg aagcgccagc tcaaaatcat aggcgccatg tacgactggt cccgggagat
     7681 ctctacctgt gatccggagt attaccgatg gacgcaatgg atattcctcc ggatgtacaa
     7741 gcggggattg gcataccgga gcacggctcc ggtcaactgg tgcccgagct gccgagtcgg
     7801 ccttgccaac gaagaggtcg tggccggagg ttgtgagcgg tgcggcactg ctgtcgtaaa
     7861 acgggatctc gtgcagtggt atctgcggat aacggcgtac gcggaacggc ttctgcggga
     7921 cctcgagcgt ttggattggc cggaacgcgt caagacgatg caggccaact ggatcgggag
     7981 gtccgaagga gcgcggatca ccttcaccgc cgtccatccg ggaaaccaga ccacctatcc
     8041 gatcgatgtg tttaccacgc gtcccgacac tcttttcggt gccacgtata tggtactggc
     8101 acctgagcat ccgcttgttc ctgctcttac gatggcctcc cgtcgcaagg aagttgaggc
     8161 atatcgcgaa caggctcgaa acgttcgcga cgtggaccgc accgccgcga cccgtcccaa
     8221 aacaggtgtg ccgctggggt cggttgccat caaccccgca aacggaaatc gcataccgat
     8281 ctggatatcg gactacgtgc tgatgggata cggaacgggt gcgatcatgg cggttcccgc
     8341 acacgacgag agggatttcg aattcgcgcg cacattcaac cttccggttg tcgaggttgt
     8401 gtatcatcat gagaccgtgc acgatgcgaa cggaagaatt caggctgcgt acactggcga
     8461 agggacaatg atccagtcca gcgggttcga tgggctcgat tcacggaccg gaggcaggca
     8521 gattgttgag atgctggccc ggcagggcaa ggccgaagca accgtgagct accggttgcg
     8581 cgactggtta ttctcgcgcc aacgatactg gggggaaccg atcccgatcg tgtactgcga
     8641 gaagtgcggt gagcaaccgg tgccggaaga gcagctcccg gttcttttgc cggaggtgga
     8701 gtcgtatgaa cctaccggca caggggaatc gcctttggcc ggcatcacgg agtgggtgaa
     8761 cacggtatgc cccgtgtgcg gcggccctgc aaagcgggaa acggatacga tgccccagtg
     8821 ggcgggttct tcctggtact tcctccgtta tgcgagcccc gacgaaaagg cggctgcatt
     8881 cgaccgcgag aaagtgaaca aatggttgcc ggtggacatg tatgtaggag gggtggagca
     8941 cgcaattctg cacctgctct atgcgcggtt cttcacgaag gtcctgtttg atgagggggc
     9001 catttctttc gatgagccgt tcacgaagct gttcaaccag gggatgatct gccgcaagag
     9061 cgagaaaacc gggcgcgttg aaaagatgtc gaaatccaaa ggcaacattg taagccccga
     9121 cggccttgtg gagaaatatg gcaccgatgc ggtccgaatg tacgagctct ttgtgggtcc
     9181 tccagaacag gatgcggaat ggaatgacaa cgggatagag ggtatcagcc gcttcctgca
     9241 ccgtgcatgg ggtctgatca cgtcgaatct ggagccttca gcaaatccgg aacggttgct
     9301 tcgggcgcgc catagacttg tgaaacaggt gacggaagga ctcgaatcgt tcaaattcaa
     9361 caccaccata agccgcatga tggagttcgt gaatttcgcg ctcgacccgg aaggcggcaa
     9421 cgggaaactc gacgcgggca cgcgggacgc gttcattgtg ttgctcgctc cattcgcacc
     9481 gcacatggcg gaagagctct ggaaactcgc cggcaagcag aacagcgtgt tcgatacgac
     9541 agtctggcct tccttcgacg aaggtctggc gtccgctgag aaaatgacga tcgctgtgca
     9601 ggtgaacggc aaaatccgcg caacggttga gctggatgcg gactcctccg acgaaaccgc
     9661 acgcgccgag gcccttgcgc aaccgaacgt ccagcggtac ctcgccggaa aggagccgcg
     9721 gaaaatcatc gttgtacccg gccgtctcgt aagcatcgtt gtataggaac gtgcagcagc
     9781 gccgttcctc gggagcagtg tccgggctcc accatgatgg agcccccttt ttacacggga
     9841 aatggaccag tgaagacaca aacaatcaat cccttcatgc cgagcaaaat cgtactaacc
     9901 aaaggcgtcg gacgccaccg cgaacaactg acgagttttg aaatggcgtt gcgcgatgcg
     9961 gggttggcag cattcaatct ggtgcccgtg tccagtattt tccccccccg atgcaaactc
    10021 gtaagcaagc gtgcgggcct tgcagagctt catcccggac aggttgtctt cacagtgatg
    10081 agccgcgcgg cgagcaacga gccgcaccgc ttgcttgcgg ccacgatcgg gcttgcgatt
    10141 cccagggatc gcaacaagta tggctatctt tcggaacatc acagcttcgg gcagacagag
    10201 gaagtggcag gtgagtacgc cgaggacctc gcggcggaga tgctcgcaac cgtgctgggt
    10261 gctgaatttg atgtgaatca atcctgggat gaaaagcggg aattgtggcg cattcagaat
    10321 gaaatcgtcg caacaagaag catcagccaa agcgcgatgg gtcacaaaga cgggctctgg
    10381 acctccgttc tcgcggcagc ggtcctgatt ccctgacgaa atcgtcaaaa ggacaccgaa
    10441 aaaatggagg gttcgccgaa tccggcgtgc cctccgtttg ctttcgaacg gggaacttca
    10501 gtatcttccc gaacgttttt gcttgaacgc ccgagccatg tgacaccctt gggtgcaact
    10561 ttcacgcagg cgcgaaccca tgaacttgtc ccaatttccc aggaatctgc aggacgtccc
    10621 gttcacgttc cttggtcttc ccgatcccga gggagggctc ggggaggcac gggtttgcgt
    10681 ccttcccatt ccctacgaaa gcaccacaac ctatcgcgga gggacgcgtt cagggccggc
    10741 ggcaattgtg gatgcctccc ggtatgtcga gacgtttgac gaggaaatgc tttgggacct
    10801 ggcagatatg ccgattctga cactcccgag tgtcagtcct cttctcgggg ccgcggaagc
    10861 aatgagcgaa cggatccggg acgtggcccg cgaggttgtt tcccgggacc gccttctgat
    10921 cagtctgggt ggtgagcatg cgatcaccgc tccattggtg gaagcgcact cggaagaatt
    10981 cggtcacctg accgttgttc acctggatgc ccacgcggat ctgcgggagt cgtatgaagg
    11041 ctcgcggatg agccacgctt cggttatcaa gcgtgttgcg gaacatacag acgttctgag
    11101 cgtaggagtt cgaagcatct cgtctgagga aatggattgg ctcaggaatc aggagagggt
    11161 tcgcgtagtc tttgcccacg aaattcggaa ggatcaaggt cttctggaaa gagtcctagg
    11221 agagctgacg ggcaatgtgt atctgactat tgacgcggat gttctcgatc ccggtgtgat
    11281 gcccggggta ggaacacccg aaccgggcgg actttcatac gatcaggtgg ttcatgttat
    11341 caagagactt tcggtgggat gctcgatagt aggtgcggac ctggttgaac tggctcccgt
    11401 accgggcaat gttgtctcgg agttcacggc tgcgcgcatt gtggcgaaga tagtggccta
    11461 cacaaagaac cctgtctaga ggagcgtttg cgatggcaaa acccaatgac gataccggca
    11521 aaacatccga aactgtcgga tatccgccgg agaaactgaa ggaattcgaa gcgtggctcc
    11581 atcaggccag gcagaggatt ctgaaaggct acgacgatca gaaagctgaa attgacggct
    11641 tcaaagggga cacggaatca gacgcgtacg atgacgcgtc aaatatcgta gaaatgaata
    11701 tcctgatggc tctcaatgac actcagaaaa aggagctctc tcagatcggg cttgcgctcc
    11761 tgaaagtgaa agacggaaca tacggcatct gcgaagggtg tggagaactc atcggagaac
    11821 aacggctgaa gttccttcct ttcgccaacc tgtgcgtcaa gtgcaaagac aaggaagagc
    11881 gtggtactgt ggttattcag cgagacacgc acatcgttcc gcccgacgag gattttctgg
    11941 ttttgacgga ctccgacgag gattactgag cgtgttgtgt caagcgaaga tactgataac
    12001 tcttcttatg taatgttgaa aaagagccgt cggcaaagga tcctccatgg caaaatcccg
    12061 aattgaaacg ttcaaggcat tcctggaaaa agatcccggc aacagcctcg cccgttactc
    12121 gctggcgatg gagctccgca aggtgggtcg ctacgatgag gcgttagccg gtttcgcgga
    12181 cctgatagag cgcgacccta agtacgtgcc ggcgtacttc atgtgcggac aaaccgcatt
    12241 tgaggcgggc aaggtgcagg aggcgaagga agtactgaga gcgggcatcg acgtcgccag
    12301 gaacgctggc gaaacgcatg cggcggagaa gatggcagaa ttgctggcaa ctctcgaata
    12361 gcggcaggaa caataggggc tgaaacaaac gagggcgcac ccgatgcgcc ctcgtttgtt
    12421 tgtgatggca ccacggtcaa tcgaccggca tcttctcgct tactccgctc ttggaggaga
    12481 tctctgctgt ttccatgatg gcgatcacgc gccttgcctg ttcaggtttg acatcaagtt
    12541 cggcgccatc gagaatgtgt gccgcgatgt tctcgtagta cttggggtgg gtgcctggct
    12601 ggtcacttac gaagccttcc gcaagcacac cgtcaaactc accataaaca cggaagccac
    12661 ccttctcgct cacgatcgcg cctttgcttc cgagcagctt ccaccttggt cttcccaccc
    12721 gggctatgtg cgacatctgt acgtccgcaa cgacattctc gtcaaaacga atgactgcat
    12781 ggacgtgatc ctcgttggtt acgtctttcc acacgaggtc ctgctgaaaa aagcccgtga
    12841 cgttttcgat cttgtttgga acaatctgga gcagccagtc gaggaaatga gcgccccaat
    12901 cgtagaatgc tcctcccgag atgtcctttt tggaacgcca ccaggtgccg gggtgtcccc
    12961 accccattcc tcccatttcc acgctgaata ctttgccaat gacaccgcgc tgtacgagac
    13021 ttttcagagt aaggaaatcg ccgtcatgtc gccggttgtg gtacacggtt aacatccgcc
    13081 gtgccttctt cgcggcgttg atcatgtcat tggcttcctt aacggtcagg cacatcggct
    13141 tctcgacgat cacgtgcttg cccgcgttgg agcacttcac cgctacaggg gcgtgctggt
    13201 tgtgtggaag gatgacgacg gccaggtcga agttgccctt ggcaagcagc tcatcaacgt
    13261 cgttgaaggt ctggatgccg ggccaatcct tttttgcctg ctctgtgcgt gcggggtcga
    13321 tgtcacagac ggcaacaagc ttcattcggc cggttgcgtg catttcacgg gtatggtgcg
    13381 ctcccatatt gaacgcgccc ccatagccga tgattgccgc tcgaacgatt ttttcgctca
    13441 tgaagtcaac tccttcgaga atgctgtttg aacagtggaa aaaaagcatg tgtggaacgc
    13501 gcaacatgta ccataatcta tggacaaagt gccgggctcc caacgtggga agaacgcgcc
    13561 tgattagggt ccgtgaagtg ctcaggtggt gtattcggcg tttatcctta cgtattcgta
    13621 ggaaagatca cacgtgtaca cctcggcttc tccctgaccg tctccaaggt ccgcgtgaat
    13681 cgtgatgacc ttttcccgga acttttcctc cgcagccgcg aggggaaatg ccgctggcat
    13741 cccattttca aacagcagca ggtcatcaac acggatcgtg agccgtccag ggttgaaaaa
    13801 cacgcccgaa cgtcccagcg cagcagcaat tcgcccccag ttcgggtctc cgccgaagaa
    13861 tgcggtcttg acgagcgggg attccgcaac cgtgcgtgcg gcaacatgcg catcttggtt
    13921 tgagggcgct cccgacacgt gaaccgtaat gaacttggtc gcgccttctc cgtctcgtgc
    13981 gatcattttc gcaaggtccc ggcacgcgtc agtcagttgt tccccaaatc tcacgtagta
    14041 atccgttccc ggtttcaacg ctggcgcgcc ggattcccca ttggccagca aggtgagcgt
    14101 atcgctggtg gaagtgtccc cgtcaatggt gaggcggttg aacgaggcct ctgcagcgtt
    14161 ccgcaacaac gagtgggcat cttcgggggc gagcagggcg tccgtcgtaa tgaaggcaag
    14221 catggttgcg agttttgggg ctatcattcc cgcgccttta gccatgccgc ctattgtcac
    14281 cgtgtgcccg ccgatggaca gaacacgctt gactgttttc gggaaagtat ctgttgtgat
    14341 gatggcatcg gcagccgctg agcccccatc ttccgatacg tcatccaacg cggcttcgat
    14401 cccgcgctcg atcctgtcca tggggagtag tctgccgatc actcccgtgg aggcaacgag
    14461 cacctgccga ggtggacagt tgagaccgga ggccacaagc gaacacattc tctccgcatc
    14521 cgccacgccg cgcgctccgg tgcacgcgtt cgcgcacccg gcgttgacca cgatcgcacg
    14581 cgctacccca ccggttatat gctgtcgtga caccgagatg ggcgcgccac acacaagatt
    14641 cgtcgtgaaa agcgccgcgg cggaggcttc tcttctgctc acgatgagtg cgaggtcccg
    14701 gcgcccgtgt gctttcaaac cgcaggccac acccgtggcg gagaatcctt tcggcgcgca
    14761 aacccctgtc cttgttcttt ccatcctgta acgtatcctt ccgtgcacta acccactggt
    14821 gaacggcgaa aacagcgcat ccggcggagc cggttgcagt caaggcttcc tttcatggcg
    14881 cggaagaagc ccgtaattaa gctatattct gaacgacagt aacacccacc cccggacgcc
    14941 atttctgtcg tggcaaccgg aacggagagg aaagtgcgcg cagacctcga atcggaaacc
    15001 agacaagcag atgagccttt tctggcgagg ttcgatccca agccggacga aagtgcgctc
    15061 tggactctgg ttagcggtat cgggcagttt gtggtggccg gctggtgcat caccatcgcc
    15121 ggcctgatga ttgtggtcgg ctatgccggc atgcatgggc ggggttccgt gctggcgagc
    15181 gcggcggcca ttctgatgac tctgtttgtg ctggtgggag ttgcgctggc ggcgatcggc
    15241 gtgcgttttc tcgcccgttc cgcaagggca ttctgggtag caatgcgtaa ggcgctgttt
    15301 gtcgtgtcgt tcaaccaggc agcagacaag cgaagggcat tgcacgcgat tgtcggcgat
    15361 cctcttctcg aaccgtacgc tcggtatctg cttcaacggg gcgatctcaa ggtgccgccg
    15421 ctgtgaagcg cacgttttcc ccgcaagttt tgttcccgct ggatgacact ccgactgaaa
    15481 tctactaact catttactgt cagcatctta catcacgttt ttgtcaatca attgtccaat
    15541 gtgttatctt ctgtactacc taatttgaag gtttgactcc ttcggattgc gtccgcccgc
    15601 aaccagggtc cggacgcgct gatatgcgga cgaagagctg cggtttgccg ccttttgcca
    15661 cactcggagc atgcgtatcg cttcggatcc catctcccct cccagttttt gtcttcgtta
    15721 tcggcgcgta acccacgagg atatgagacg gcgaggggaa ttcaagtagt cttgcaggag
    15781 gactgctgtg gcttcacgaa cggttattct gataaatccc gaccgcgaaa aacccctcat
    15841 tgctcccctc gggcttgatt acctcgcggc gacgttgcgc cacgcagggt atgaaccgga
    15901 gcttctcgat cttgcgttcg agagtgattg gtacgccgca acgaatcagg cggtatcgaa
    15961 gcgcgatctt ctggccgttt tgatattgat tcgtcacctt gacgacggtc tgtacaacag
    16021 ccagacgttt ttcctcccga agtcccggac tctgatcgaa tatctccgga gcaaaacaaa
    16081 ggcgcctatc ggcgttatcg gcggggcgta ctctctcgtg ccggaagcca tcctgcgatt
    16141 ctgcagggcg gatttcggga tcgcgaatga cccggaagtt gcccttccca tgcttctcga
    16201 ggcgatcgaa tcaggcgatg ggtacgaagg tgttccgaac gtggggtgga tcgaagggaa
    16261 agcgttccgg cggaacccga tcaagacggc gcgcgtcgac ggggaatggc ttgcagaacg
    16321 aagtctcatt gacaacgttc ggtatttcaa tgaaagcgga gtaacccggt ttgagaccaa
    16381 gagaggctgc gcgcacaacg aagtctattc aaccgacccg ataacgcgag gtaacagcgt
    16441 tcggctccgt ccggcggagc acgttgccga ggaagtggaa cggctggctg cccggggcat
    16501 ttacagcctg tatgctgatg acacgcgatt caacgaccca cgtgagcacg cgctggaggt
    16561 gtgccgtgcg atggccgagc ggaagctcag ggatcgggta cggtggtacg ccacatgcac
    16621 acccaagggt ctcgatgccg aactcgcgga agcgatggaa gctgcggggt gccagggtgt
    16681 cgagctgttc accgattccg gcgacgcgga ccagctccga cggatgaagc acacgcatac
    16741 tccggaagac atcctcaccg cgatcgagtc ttgcaaacgg tacgggatcg tcgtaatcag
    16801 cgaggttcag ataggcggcc ccggcgagtc ccgcaaatcg atagaacgga ctctggggct
    16861 catgaaagct tccgggcccg acatgatcaa tatccactac gggcttcggg tgtatccgaa
    16921 cacgccgctc ggcaacgcag ttcaaacggt tacgcctttg aggaacaact tccacctccg
    16981 gggagcgatt tacaacaacg aatcgctttt gcgtccggtg ttttacatcg aatccctgct
    17041 cgggcgcgga gtagaggaat tcatagagga attgatcgac cacgacccac ggtatctttt
    17101 tgcgttccgc aaagaggttg acaagacgta caattacaat gaccccacaa ttctgaccga
    17161 ggcaatcctt cacgagggcc atcgcggggc gcaatgggat atctggcgcc gtctgattta
    17221 ccgtcttccg ccgctttcac tgaacggtga ccgtccctcg cgaggaaagc gacggccgcg
    17281 aagggtcatt caaagcgccg aagcgggcgt tgctgtttga cggtcgttca cgcgaacttg
    17341 tgcagacgaa agggggcccc gatccccctt tcgtcttttc cggcataccc cgaaaatgag
    17401 gctctgatcc gatgaaactc ggtacctgtg cgtattccta ccgcgacctg ctcaccaagg
    17461 gcaagatgac gctgatcgaa ttgctcgaca ccgttcgcga tctcggattc gatggcattg
    17521 aatggaccgc gtactatttc cccgagacga cgaaagaata tctcctccac atcaaacggg
    17581 agacattcgt gcgcgggttg gacatttccg ccacggccgt cggcggcaat ttcatccaac
    17641 cgacgagaga agagcgcgaa aaacagatcg ccaatgtcaa atcctggatc gaaaagtcgg
    17701 cgtggatggg atcaaccgcg ctgcgggtgt tcgcaggata cgtcaccgaa gggatggcgc
    17761 gggaggatgc cgatcagatg gtaagggatg gacttgccga gtgcgccaga cttgcggaga
    17821 aagaaggcgt aatcctggcg ctcgagaacc acggcggagt cactgcagat gccgagggca
    17881 cgctgcgcct cgtgaagccg ctgaacaacc cctgggtgaa gctcaatctg gattttggca
    17941 attttaccgg tgatatttac gcgcagtatg aagcctgcgc tccgtatgcc gtaacaacgc
    18001 ataccaaggt aaccgttcgt cagggtgagg cgcgcgagaa ggtggactac cgcaaagtgg
    18061 tgcggatcat gcggaatgcg gggtaccgcg ggtacatcaa catcgaattc gaggaacccg
    18121 aggatccgat tgtgggagtt gacaggtttg cagcctacct caggggatgc atggaagacg
    18181 catagttgag cgcaggggga ttgaagaaga agttggcgcc cagagctggt ttcccacagg
    18241 gccagaaaga aagagatgtc tggaatgaaa cgcccatttg cccttttttg tactgctttg
    18301 ctcctcgttg tctctggttg tttgggtacg atcccgctcg gcgaacgggg gcgggcggct
    18361 cacgttcttc gatttgacga caatggcgtc tttaccatcg tccagttcac ggatactcat
    18421 ctcggttccc gtgatgccgg cttgcgcgat gactccacgt acgcgctgct ggactccata
    18481 atccggtggg aaaacccgga ccttatcgtg ctgacgggag acatcgtccg attcgcgtgg
    18541 gaaccgcagg ctgtaccctc ctggcgccgc ctgattgcct tctttgacag cactggtgtg
    18601 ccctgggcgt ttgtgcacgg caaccacgac gccgaggtgc gggggtacgc cgcggtggat
    18661 agtctgattg ccactgcgcg gaactgcctg tatacgtcgg gacccgacag cctcaatcaa
    18721 ccaggtaact atgtcctgcc ggttctttca cgaaaaggcc gtggggttag tgccctcctc
    18781 tggtgcatga actctggcgc gggaagctcg gagccgtccg ggtacggttg gattcgtgcc
    18841 aaccaggtca aatggttccg cgccacgagc gaacgcctga agacccagac ccaaccgaac
    18901 ctcacccaac tggcgttttt tcacataccg tttgcacaat atcagacggt ttgggatgca
    18961 cgcacatgca ccggttacaa gaacgaaaag gtctgcatgc agggtaaaga cgaaggcgcg
    19021 ttcgccgcgt ttgttgagta tcgtgtgaac gggtgtttcg taggccacga ccacacgaac
    19081 gactacgagg gtactctgga agatgttact ctgtgttacg ggcgcgcctc tgggtaccgc
    19141 ggttacggca aagaaggatt tctgcgtggc gcccgcgtga taaagatcaa agacggagta
    19201 cggggattca cctcccacat caggcttgcg gacggttccc tcgcggaacg gccggtgcac
    19261 actcccggca cggctctgcc gactccttaa aacctgaacg attcgggttc ccggttcgtc
    19321 ccgggagccc gaatcgctat attaatcctg ctgatcccgg cattgcccgg gtatcccgat
    19381 gtagttcaat tcccccggcg atccagcgac ggttgcccgt tccgaaagga gcaattgatg
    19441 gcaaagcaga ccgacatgta cgacccgact ctgaaggatc agagtgaata cgaccacaag
    19501 gagatttaca tcatctccaa cacgcactgg gaccgggagt gggtttatcc ctcagcggaa
    19561 accagactgc tgatgctcga gttcatggac aacatgctcg atctcctcga caagcagccc
    19621 gactatcatt cgttcaccat ggacagccag acactctgca ttcaggacta ccttgacttc
    19681 cgccccgaac gacgcgagca gattgaacgc catgtgaaaa gcgggcggct gatcatcgga
    19741 ccatggtgca gcctgccgga ggaatacatt gtcaacggcg aatcactcgt gcgcaatctg
    19801 gtgatcggtc acaaggtggc aaatagcctt ggaaaagtat cgaaagtcgg ttacacgccg
    19861 ttcagttatg gccagacaag ccagatgccc cagatctaca gaggcttcga catcgacacg
    19921 atcatcttct accgcggcat caaaacggag aagagcgaat tcatcatgcg tggcccggat
    19981 ggcagcgaac tccttggtat gcggttcggt gcgctctcca gattcagcta tttcttctat
    20041 ctcttccgaa tggtcgccta caacatgtct cgggacgaag cgaaatacga ctggttccgc
    20101 ggcagcctcc cgttcaaact tgccggtgac caccatgtaa cgcgcattac tacatatgcg
    20161 atccggatgc cacgggctgg cataccgagc acattgatac aggcgcaagg aaactcctgc
    20221 gcgacgaatc ccagcatttc agcacgcggt atatcgcgtc catgcagggt tttgatagtt
    20281 ccgaacccga tccccgcgag ctcgacctta taaaagagac acagaaacac ctcccgaacc
    20341 acgtaattaa gcagtccgac ctcgcggagt tcatggatcg cctaaaggaa gaggttgctg
    20401 cgggtcggtt gaagccggag gtaattgaag gcgagagccg gaacccgaac tctgtgggga
    20461 agtggacgca ccttttcggt gatgtcattt ccagccgtgt gcgcatcaaa ctcatgaaca
    20521 acgcgaacga gcaaaacgtc cagcgcaagg ccgagccgtt ttgtgccctt gcgatgacac
    20581 ttggtgccaa ataccccaca accgtcctcg acgagtgctg ggacaacatc atgaaaaatc
    20641 acccacacga caccatctgc ggtgccggcg tggatcagat ggaaaaggat atgatcgccc
    20701 gcggggaaca ggtcaacatc ttcagcgagg gcttgtatcg ccgggcattg cagaagattc
    20761 agacgcggat tgacaacagc gacgttgccg ccaacgagat tgccctgacc gtgttcaacc
    20821 cgtctccatt cccgcgaagc gaagtggtga gcgtgctcct ggatttgcca gatgtgtgca
    20881 aatatgagga tttcaccata cgtgacgaaa aagggaacaa ggtagaagcg cagtacgcgg
    20941 agttctatcc atggggaacg ctggtgcgca acctgcgcga cgtctctcta cagcttcatg
    21001 ccgaacgtgt gcgggtgcac ttcgaagcct tggatgttcc tccgttcgga tatcgaacct
    21061 acaccgtcaa acacgaagaa gacgtcgtgc cgaaaccgat gaagacgctc gttacggccg
    21121 tcaacacgat ggaaaacgag catctcaaag tgaaaatcaa cgccaatggc actcttcaga
    21181 taaccaacaa agaaaccggt gatgtatttg acaacatgca ttacctggag gacggtgggg
    21241 agaatggtca ttcctggacg accatccccc cggatcatga cgagatcatc acgagccacg
    21301 gcgcgaacgt atacattgcg cagatggaaa gcggcccggc cttggcgcgt ttccgcgtag
    21361 aataccacat gatgattcca gaggcgctcg atttcgaaac catggaggcg tacaccggcc
    21421 agacgccccg tgcacagatc cgccacccgc gcataagcaa gcggagcgaa cggcggaaag
    21481 agatggtgat tcgttcggag ttcacgcttc gcaagggctc aaaggtgctg gaagtataca
    21541 cgaaggtcaa caacgagtgc gaaaaccacc gcctgcgtgt atgcttcccg accggtatcc
    21601 aggcagcgac gtcggactca gaagcctcgt tcgacgtcat tcagcgccag gtgatccgca
    21661 caccggacaa ttggtactgg ggccgcccga atcccgtgtt gccgatgcac cgttttgtga
    21721 gcttctcaaa cggcaaaagc ggcatcacgt tcctgaataa cggtattcgc gaatacgaag
    21781 caatggaaga tgccgaccgg acagtggcgc ttaccctttt ccgggctttc acattccgaa
    21841 atccacccat cctcgacacg tatgacgtat atccggagat gaagctttcc cagtgtcccg
    21901 gtgagatgga gttcaggtac aacatctatc cgcacaaggg agactgggcg gctgcagagg
    21961 tgtacaaaga ggcagatcac atcaacctgc cgcttgaggt ggcggaagtt gggccgcaca
    22021 agggcgacct tccgaagagc atgagcttcc tttcgatttc cccggccaac ctcgtgttgt
    22081 cggccttcaa aaaggctgag gaaagggaca cgttcatttt gcgcgtgttc aaccccactg
    22141 gcgacgcgat gaatggaaca atcacgttct acaggccgat aaagaaggca tggttgtgca
    22201 atatgaacga ggaacggcgt caggatctgg agccgaaagg gaacgttctg accctcgatg
    22261 ttccgaaaaa gaagatcatg actatcgagt tcgaagcctg acgcctgtcc caagaacgga
    22321 ttacgaaggg gctcccgcga tctggtggga gccccttttg tttctcgcac gtttttcgac
    22381 ttcgtgcctg gaacgatggc ccgcgtgaga accaaatcgg cgccggcgga gtccgcacat
    22441 tgccgacacc gcgctcgatg tgatatattc aagaaataac gaagcttaga gacacgaagc
    22501 acaacgcagt agattccggg gcaaatcgat gacaaaaacg cgggcttctc gcacggggag
    22561 cccgcgctgt tttggttccc gcgtgacccc atgaagggtg cgtcatggca aaagcaatca
    22621 gtctcgcgcc ggtgattatc gccatcattc tggcggcttt cggtcaggtc acaatgaagt
    22681 acgcgatgtt cggttcgccg cttcccgtaa catcaccggt ggaagccgcg aggaacatcc
    22741 tcagtcgccc gatggtctac gtgggcctcg ttttctatgc gggatcttca ttcctctggc
    22801 tgatatcgct ctcgcggctc gacctgagtt acatgtaccc cttcacggcg ttactgattg
    22861 tcatcataac attcgtgtcg gctctggtgt tcaacgagcc gatcacactc tggaaggtgg
    22921 tgggtgtgtt cctaatttgc gcgggattgc tttcgatagc ccgcagttga cgagccgctc
    22981 ccccacactg caagcatggt agagtgtgca caggttttgc ttttcgtgtg ggctctgatc
    23041 cattcgttct gtccgttccg atttgccctg gttcgcacgc gtgcgaactg gcaaatgtaa
    23101 ggcaacgcat ccttctatcg gggtgcgaaa actgatgaac gctcttgtga aataccttct
    23161 cgcctttgtc ctcattttct gtggtttcca gattgcctca gcggaggcac cgccaacgga
    23221 tactctcagg ttgaccttgc gggagtgtct cgaccgcgca atgcagggga acctggatat
    23281 ccgggaggcc cagttggaac gggaccgcgc tctgatcgac caactggaag ccaaccgcgc
    23341 cttctggtta cccgtattcg aagcaagaag ttacctgagc gtcgtgaagg acgcgagggg
    23401 atacgtgaca gatcatccgc tcacaacgtc gtgggacaac ttcggacctt gcttcgagtt
    23461 ccacctgcag gcggctcagc ccatttcgac tctgggccgt gtcaatgcgc tgaaacgagc
    23521 ggcccggaac gggatcgccg cccgc
//
LOCUS       DAOSOO010000027        22275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2333_length_22275_cov_6.241719, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000027 DAOSOO010000000
VERSION     DAOSOO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 22275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22275
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_22275_cov_6.241719"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLT86_03480"
     CDS             complement(<1..745)
                     /locus_tag="PLT86_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOT35709.1"
                     /translation="MRKELSLNGCWKFYPAFDEVSGDQKWMDPDFDPTNFEITPKEGM
                     AIGWIKEGFDDSGWLDSPVPSSWNAAFEDLWSYEGHGWYRRKVLIPNEWEGKRVEFFS
                     EGANYRTALYVNGDFAGEHFGGYTPFGFPVHKLLKFGEINTIAIECDNVPKSNRCPGG
                     QFDWWNEGGLYRDVCLRLTDPTYIDDVTVVTRVSSDDVEITVDIEVCSDEPIFPERSI
                     ALRLSDPAGDYVPVPTSVSRVVYDAPLKKG"
     gene            complement(791..2044)
                     /locus_tag="PLT86_03485"
     CDS             complement(791..2044)
                     /locus_tag="PLT86_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35710.1"
                     /translation="MTPRERVRHTLSHREPDRVPIDLGGMGSTNIMAIAYNRLKAFLG
                     IRGGTTRLYDWGQQLCEPESWAIERFHLDVVALSRSLSPVSDPERTREFEARFGGKWK
                     PWVLPDGSLCEAEESFNPEPDGSGGWQMRNERGVWAVMPKSALYFDGGPANHPLDHAK
                     SVADLGDWKPGYAHEDSYKTAAEKAKWLHENTDFAIMAGFGGGALEPAQGLRGWGQFM
                     EDLAGDPDLADEIIGRLTDANVHNVRLFMEMVGPYLDLVQMGDDLGTQAGPQMSPAMY
                     RRFIHPAHKAVYQTAKKTAPNVAIFLHSCGGIRPLIPDLIDEGVEALNPVQTNAAGME
                     PEGLKRDFGGKLTFWGGGAEVAGVLTYGTPDEVRAQVRERIRIFAPGGGFVFCQVHNI
                     QANVPPENIVAMYEAAYEFGKYPIS"
     gene            2359..3447
                     /locus_tag="PLT86_03490"
     CDS             2359..3447
                     /locus_tag="PLT86_03490"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35711.1"
                     /translation="MGLTGSFRGLSITEMLQLIRMSKKTGALRITLVDNTRHTIYFRA
                     GQVIGVECGRDSLIPFLLERGLLSNDDLNAAISQQKSSSRRAPLEQILLDSGAVTRDD
                     LVQALTSHAEETIGALIQEGGGTISFEDGAQPQLKLEIALDIHNLLMAASVKADEALR
                     DRTLPYVPTDVYAHSGQGKTAFAEKTMRLWVDHWRVFLAIDGNRTLRAISEKIQVAPD
                     ELMKGIAALLTLGYIEPVAGGRARRIALVVDESAMVQKLVQMALAEEDMEIESVSDAD
                     SAHRVLAERTPDVILMNVALPDTSGYRLCRQLRGGDSPCNGVPIILLSARDTPQDEKL
                     AIHAGATAFVSLPFAPESLRNTVRSVLS"
     gene            3534..3884
                     /locus_tag="PLT86_03495"
     CDS             3534..3884
                     /locus_tag="PLT86_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35712.1"
                     /translation="MALPDSPRNRELEAWLRDVTAGIATTILAEVSEDRILAEMESKA
                     GVSDETVVRWGPGVQGQLTGTGKESLNGGNASADGTDRYRFRRPVSEDQGRAVLLFLA
                     SLFGLSDPSEQGDE"
     gene            4029..5159
                     /locus_tag="PLT86_03500"
     CDS             4029..5159
                     /locus_tag="PLT86_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35713.1"
                     /translation="MPEERRLLVVDDEEVITWAISQALTRPGEMRVVTARSGEEALEL
                     MAKEPFDLVVTDIRMKGMTGFELLSRIRETYPESGVIVMTAYGSPEAKREATERGSLF
                     YLDKTFELEEFRSVVRKALEQVDRGRAAVGKGEQEGFSGLIGNLKLIDIVQLHCLARN
                     TAMVEVRAGKITGRVGFLDGEIVFAETSPGEKGRTAFLDIMSWDTGEFAIIDEAPAEQ
                     NIDESWESLLIETTALMENSTGEPDVPAPAGADSAEFETPADIHTIVEDLIHTDGIVG
                     AVVAGDTGIVVDSAFDGWDGDDNGIAELASCMAGISRVRFVVEGETQRSHVIIQFDRR
                     RVIAQEVGELGIFLLVISSGTGGLAQHLSATDTAAKKLARLL"
     gene            5397..5744
                     /locus_tag="PLT86_03505"
     CDS             5397..5744
                     /locus_tag="PLT86_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOT35714.1"
                     /translation="MKKVLQELGEVEGIVGSLIVDADGIPIVECLDADSELVAATCVQ
                     IVNSVRDALAKLEEDRLVQALIEPAEGKIFLREIPDVGTLVAVSEKNVNVGLIRLALS
                     SASEKIRAYREAL"
     gene            5863..6543
                     /locus_tag="PLT86_03510"
     CDS             5863..6543
                     /locus_tag="PLT86_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HOT35715.1"
                     /translation="MPTIDYANKEISCKIVYYGPGLCGKTTNLQLIHRDVPGINKTNL
                     VSLATEKDRTLFFDFLPVTLGEIKGFKIRFQLYTVPGQVYYNATRKLVLSGVDGIVYV
                     VDSQRSKLQENLDMMQNLRENLAEYRMDLATIPMVLQYNKRDLPGIVPVEELQRAMNP
                     RGLPWFEAVAVQGIGVKETLKAIATLTLSRIGKRTQAAPRDGAAAGPSNATDTGQPKR
                     SIFRKTNP"
     gene            6644..7150
                     /locus_tag="PLT86_03515"
     CDS             6644..7150
                     /locus_tag="PLT86_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOT35716.1"
                     /translation="MGGGGVVIYDEQLTRINSLIGHLLKESDAKCALLVGKDGRTIAR
                     QGFTRTIDTDQLGALVAGAFSASKAVAELIGEPEFSTLFHQGKHESIQIHLLDPRTLL
                     VVTFDERTTVGMVRLVTKAVSGSLREILQAVYDNPDVEEIGTPEEIASTFDGVFGDSE
                     PKGGSKQP"
     gene            7190..8887
                     /locus_tag="PLT86_03520"
     CDS             7190..8887
                     /locus_tag="PLT86_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT35717.1"
                     /translation="MRHSEHLRTPPCHPRLRVLFDLLLFCLVIFVTAPDSAVHAAWAV
                     YGDSWSSATQAQEQVTRVNRVLGEQQASYERATVQGRTYWRVRIGVYATRNEALEARR
                     RAITAGVKEPWMREVSDAELAVQTPSPTPSQTAYADSVRADSLRKVFMDSLARATRRR
                     IDSLGTAAQRQTMDIIEQMRLQITQELVERIEREQARDRSSYVTTAEQSQMTNALIDE
                     MSSRFEVLRDSLRTDRAMRDARDALLPRISGYVAATGIASHDRPAGAGISTSAEVAIP
                     HAQALFEWANDRGGVVLDVRTAGALAVELHQAFVTWSFGATGGASRSLGLGLCEMPYG
                     LEPSFTSDLLTCSPSQVSAAIPPPARGLLLVPHDGERVTLSVFPFGRWDEGTQKGLVQ
                     LALHNEATRFELTAGGERVRYQNEVAPRRYEFAGMANAVIERKGANWLLGAEANLERR
                     RQPVSGILAVNRRQVDTWGGLALVHRRFSERWGWTLRGDFTTQVTTWTNLAVTAPPRE
                     PLARNWTVTSGPIFFLGDRLRARVNYTFDLQEERAVGTYALERSHHHRFEFGLSHVF"
     gene            9095..9661
                     /gene="infC"
                     /locus_tag="PLT86_03525"
     CDS             9095..9661
                     /gene="infC"
                     /locus_tag="PLT86_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT35718.1"
                     /translation="MAPVQKNEDRTRVNQHIRVPEVRLVGADGEQIGIVNTREALRMA
                     EEQGYDLVEVAPNALPPVCRLMDYGKFRYEQQKKTRAITKNRGGEQKEIQMRPNIDLN
                     DYQTKLRHAKEFLAGGDRVKLTVRFRGRELAHRDIGRDLLERFAKDLAGDGRIEGGVF
                     EEGRNTSLNVVPSRSGGSGAAPASASPA"
     gene            complement(9757..10674)
                     /locus_tag="PLT86_03530"
     CDS             complement(9757..10674)
                     /locus_tag="PLT86_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35719.1"
                     /translation="MSAFTLSLAKFIPFQDAAECARVRAIKKKDLTRHPNPEFRIRII
                     ENIADLNLAFALDIVQRIKKARDEGRKFVAIFPVGPMGQYPIAAEIINSMRLSLDHVV
                     SFNMDEYADAEGNTAPADWEGSFQRAMMESFYGRIHKNLRPSEKNIHFPTKAVIKNYG
                     KMIEDEGEVDVVYGGVGWCGHIAFWEPHLGFEFGNDLDAYMKAGPRLVELHPMTIMQN
                     ALHSFGGDWSWVPPKANTIGPAQIVRAKHRSFWLDGAFGKDSWQRFIARFASHGRVNT
                     LVPATILQTCRTDFTILGAVADNVEVHMA"
     gene            10857..11522
                     /gene="plsY"
                     /locus_tag="PLT86_03535"
     CDS             10857..11522
                     /gene="plsY"
                     /locus_tag="PLT86_03535"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOT35720.1"
                     /translation="MSALLLASVLSYLLGAFPTAIIVGKLLRGIDVRDYGSRNAGATN
                     VWRVLGAGPALFVLAIDALKGAGAVLLIARIASDCCPWDPLTTQVICGMMAVVGHIWT
                     VFAGFRGGKGVGTGAGVFAALAPWCVLIATVSFVAVVALTRYISAGSITAAFVLAAGL
                     VIQRLFFPESLPIPVVVIGCVVAAVVIVKHRTNIQRLINGTENRFGKPASATLAEKEG
                     HRS"
     gene            11519..12523
                     /locus_tag="PLT86_03540"
     CDS             11519..12523
                     /locus_tag="PLT86_03540"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT35721.1"
                     /translation="MNVVVMRAGSWGSVLSGLLARNGHRVSLIAPHEALRTELLTYRE
                     NRKALPGVPIPDTVAILSAPDDVLESADMVVFPGPCELMRDMARSVKSANWQGKLVVT
                     AAKGIEQGSFKRMSELLAEELPEVPPDEIVALSGPSFARFVGLGHPTTVVAAARSEKA
                     AVAAQRAFMSSTFRVYCSPDSLGVELGGALKNVIAIAAGMSDGLGFGDNTRAALMTRG
                     LAEITRLGLAMGAQLPTFLGLSGVGDLVLTCSSRESRNHFVGEQVGKGRTLKEVLQGM
                     VTVAEGVKTAPAAIGLAEKWGVPMPIASEVNAVLFEEKKPQDAVTALMMRDPKPEHWS
                     "
     gene            12568..14559
                     /locus_tag="PLT86_03545"
     CDS             12568..14559
                     /locus_tag="PLT86_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT35722.1"
                     /translation="MLKRRFTRLYWRIGEVAEITGISQQTLRAWEQELAILRPRRDAS
                     GVRVYRERDFRIVLVLQQLIEKEKWSVSDVKELLVAAPDEIRAMINAVPPEALEGTPD
                     ENEPSTTARTVEKPEVAATPHEDKNEEPAQAGVRLAGAAVPRETEQEVTEAPAEEPGS
                     GGGEVVETPAVGAAAYPPAETEERELAGASSPTLPLTEEPSTVVDAAEAEETAEPASG
                     EMSAEERSFPAEGVSETEETWQDTGLPPFATETPAEPEREPVEAPTSTEPVVETSDGD
                     MDLAASAPPIEREELAPVEVQDATPSVPVEANLMEQPVLPGDQIATAEDVSPATETGY
                     HPEEEDREQVFAAAEQIESFVHSEGMAEETAGGPAAGEATEEIGMAAPVWTPVEEEGE
                     ASLQGEPEAATPREASSPDSEFEPEPELAESGEKEHLNQPVVPEVAAEESEPSPAVQP
                     WDGAPVTDVAETPPSTSIPEPEPLPSATPTIGEAGSEILAAAETAAQIAESVSLEAPE
                     NEGADGTRDSIANMAFPQEQPESAPETGAPSDANVASETEPGETDTEEEEAISPLLAM
                     PADTQTELSTHEEIEPPRPERREVFELIASEAVPVPETLPKSVVEDLRALREELAAIA
                     EMVRMLVEPSPESTEATKDVSDERALAECSSGDEKSSDA"
     gene            14580..15554
                     /gene="xerD"
                     /locus_tag="PLT86_03550"
     CDS             14580..15554
                     /gene="xerD"
                     /locus_tag="PLT86_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT35723.1"
                     /translation="MERKSVKSSPRIARDASRAAESSPAQLPGLRLLSSFLQYITAER
                     GLSQNTQAAYRRDLDRYVRFLETKRGGMTPRTATREDVSSFFSWLRGQNLAPSSIART
                     MSAVRVFHRFLQNEGVAESNPTGTLDTPKLERNLPEVLSRNEIERLLNAPDPVTPLGL
                     RDRAMLECAYATGLRVSELISIDIRNLFFNEGFIRVTGKGNKERLVPIGRKAVECISA
                     YVATARSAVAKPESGNVLFLNWRGRPLTRMAFWQMLKNYCRKTGITKTVSPHTLRHSF
                     ATHLLEGGADLRAVQEMLGHSSIATTQIYTHVDREHLKAMHRQFHPRG"
     gene            15615..16805
                     /locus_tag="PLT86_03555"
     CDS             15615..16805
                     /locus_tag="PLT86_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOT35724.1"
                     /translation="MRESFSIPLITLFGFLLLACATETYAAGTLGQLDEQRLRQTVDS
                     LYGSPYRYGGRSREGFDCSGFVGTVFQDQGIELPRSSREMYRVGDPVKRNQLAVGDLV
                     FFRTRGRRRGVSHVGIVNGPDSFVHANTRRGVITERLTDPYWKARYVGARRVATPVSP
                     PPAQEIAEDSYPFNTSSLVNTPTIALCPGKGLSALSFATGFRGDVAISPEFTFMNRLQ
                     AGASVRLRRAVGTGTPSIAVPSIRAKLRINEQIGQIPGFAIGFDSERDELTYSTALGD
                     STASSPRRGLFAVGSGTLVYPRSFFLGRGTGHAGASLSSFAGNGIKRNLSFFMGVDQQ
                     LLQRMTLLGEIDNIMGVGGWRANVGARFSITDDAVLEYAVLHMAGDNIRPDKVLKFSF
                     SVPY"
     gene            complement(16883..19969)
                     /locus_tag="PLT86_03560"
     CDS             complement(16883..19969)
                     /locus_tag="PLT86_03560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOT35725.1"
                     /translation="MSYAALWCKSNFSFLEGASHPSELVEQAATLGLHAIALTDRDGV
                     YGAVQALLAAESTGARLIIGSEISLDDGSTIILLCRTRKGYSNLCRLISAGRMRSPKG
                     ESKVSWEEVCSHSEGLLALWGGDRSLLLRKADPDIVARQLKEAFHDHLHVIISRHRRA
                     DDVAKEALLRLRARKYDLPTLAVPEVLYHEVSRRPLQDVMTCIRHGVTLSSAGRRLKP
                     NAEYDLKSPARMKRLYRDDLESLERTCAVADSCTFTMREIAYRYPSGQLPNGMTGEEW
                     LRKITYEGAAQRYPDGIPDEVTLQVEKELSLITELKYTGYFVTMWEIVRYCRREGILC
                     QGRGSAANSAVCFCLGITAIDPVRMGLLFERFISRERAEPPDIDLDIMHERREEVIQH
                     VYDEYGRSRAAMVANVVRYRAKRAIREVGKVLGMAESACDRLAKLTSSYGDLTDTSLR
                     DAGLDPDQPLHRHLLTISNEIIGMPRHLSIHPGGFLLGAEEILDIVPVENATMEKRTV
                     IQWDKDDVEALGLFKLDLLGLGALTQLDICFRLLASHRGVNLSMATIPADDRSTFEMI
                     ARGDTVGVFQIESRAQQAMLPRLRPRNYYDLVIEIAIIRPGPITGGMVHPYLRRRNGE
                     EAVIYPHPCLEPVLQRTLGIPLFQEQVMKLAMVAADYSPGEADQLRRDMAAWKKKGGL
                     ERHHDRLVNRMREKGIDKAFAERVFEQIKGFGEYGFPESHSASFALISYASAWLRCHY
                     PAEFLCSILNAQPMGFYSPATLVQDAIRHGVSVRPVDVSWSSWDCTLEPLPDGEFAVR
                     MGLRHVNGLGKKDAETIQNARINGPFSDIDDFVRRTSLDEGVLRSLAQAGGFESLERH
                     RRQAVWRVKGASRTDTLPLAVAEPDPEPVFELLSEADKTAWDYRASGHSTRAHPLLAL
                     RPLLSAQGILSASEIRALQDRSLVRYAGLVICRQRPGNANGVTFMTLEDETGHANLII
                     WKDIYQRHRILAKTTSFLGVTGEIQSRSGITHLIARRLWVPRVTPRPEVVKSRDFH"
     gene            complement(19969..21384)
                     /locus_tag="PLT86_03565"
     CDS             complement(19969..21384)
                     /locus_tag="PLT86_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35726.1"
                     /translation="MPLQILCLRHSEWWGNPVAVITRDAPQAEILYMNPVARKMGVRT
                     GMRYAAALGLAPQLHAGTVSAEDLAGCIALLSRRMRTFTPDVEPDAGEPGVFHLNAGG
                     LTGLFGSETAWSKEVWKAVRSEGFVSSIAVGFTRFGTYAAAKTRKGESVFPSPELERK
                     AALAVPLHALGLPPDTTQELSLLGVNTVDNLLHLPVYGLRLRYGEQVFCLYKSACGDL
                     WQPLQADAEKPLHARRMIFDTPERDTQRLCRFLEKMLTTLAGEVEKKGELIGSIHWTC
                     TLDTGLPYSSEVTPSNPTGNIQRLMELIHLRIEAGPPPSAVEEIVLSVQSSPAYTAKR
                     DLFDVPEPRNRVAGERALARIRAAFGDDAVVTAHLADEHLPERSFHWDIARSVPVPNP
                     CDVSEEFPYMRRIYTVPQRLPDEYRKTGNRVVGEYRISGDWWSRKTVREYRFILTNEG
                     RLLWVYYDYSRADWFIQGEVE"
     gene            complement(21439..22101)
                     /locus_tag="PLT86_03570"
     CDS             complement(21439..22101)
                     /locus_tag="PLT86_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HOT35727.1"
                     /translation="MLREIPIKSASALHIHADRSEWRYKELRGRLTEISAWENPAALS
                     SVMMLVRQAQCAGEPTAWVGKASDIFFPLDAAAYGVDLAALIVVRLSQLSDAPRAADM
                     LIRSGGFGLVVIDLSDASPVCIPMPLLSRIVRLSHVKNVATVCLTRKPVHASSLGSLI
                     SLRTQARRVGKFDATAGISLEAIKDKQHAPGWVHREYRGVPVGMRSDNRFTAPIALCK
                     AS"
BASE COUNT         4799 a         6359 c         6622 g         4495 t
ORIGIN      
        1 ggcctttctt caggggtgcg tcgtacacga cgcgggaaac gctcgttgga acggggacgt
       61 aatcacccgc cgggtcgctc aggcgcaggg caatgctgcg ctcagggaag atcggttcgt
      121 cggaacacac ctcaatgtcg accgtaatct ccacatcatc tgacgagact ctggttacca
      181 ccgtgacgtc gtcgatatag gtggggtcag tcaggcggag gcacacgtcc cgatagagcc
      241 caccttcgtt ccaccagtca aactgcccgc cagggcagcg gttcgacttt ggaacgttgt
      301 cgcattcaat cgctatggtg ttgatctcgc cgaatttgag gagtttatga accgggaatc
      361 cgaacggagt gtacccgccg aagtgctctc ccgcgaaatc gccgttgacg tagagtgcgg
      421 tgcgatagtt ggccccttcc gaaaagaact ccacgcgttt gccttcccac tcgttcggta
      481 tgagaacttt tcggcggtac caaccgtgtc cttcatagct ccacaggtcc tcgaatgcgg
      541 cgttccagct gctgggaaca ggtgaatcca gccaccccga atcgtcaaat ccctctttga
      601 tccagccgat ggccattcct tccttcggcg tgatttcgaa gttcgtggga tcgaaatctg
      661 gatccatcca cttctgatcc ccgctcactt catcgaacgc agggtagaac ttccagcagc
      721 cgttaagact gagctctttt ctcaaggtgt gtcactccct ttttgtgcgc agtgccaggg
      781 gtacatcgtc tcacgaaatg gggtatttgc cgaactcgta cgccgcttca tacatggcga
      841 cgatgttttc gggtggaacg tttgcttgaa tgttgtgcac ctggcagaag acaaatcctc
      901 ctcccggcgc gaaaatccgg atgcgctccc gcacctgcgc tcgaacttcg tccggggttc
      961 catacgtcag cacccctgct acttcagcac ccccacccca gaatgtgagc tttccgccga
     1021 aatcgcgctt caatccttcc ggttccattc ccgccgcatt cgtctgtacc ggattcagcg
     1081 cctcaacacc ttcatcaatc agatcaggta tcaacgggcg tatcccaccg caggaatgga
     1141 ggaaaattgc gacgttcggg gctgtcttct tcgccgtctg gtaaactgct ttgtgcgccg
     1201 gatggatgaa gcggcggtac atcgctggcg acatctgcgg gcccgcctgg gtcccaagat
     1261 cgtcccccat ctgaacgaga tccagatacg gccccaccat ctccataaaa agtctcacgt
     1321 tgtgaacgtt ggcgtcggtc agacgtccga tgatctcatc cgcgagatcg ggatcgccgg
     1381 cgaggtcctc catgaactgt ccccaccccc gcaacccctg tgcaggttcc agagctcctc
     1441 cgccaaaacc ggccatgatc gcaaagtccg tgttctcgtg gagccatttt gccttttccg
     1501 ccgcagtttt gtacgagtcc tcatgtgcgt acccgggctt ccagtctcca agatccgcca
     1561 cgctcttcgc gtgatcgaga ggatgattcg ccggcccgcc gtcgaaatac agcgcacttt
     1621 tcggcatgac agcccacacg ccacgttcgt tcctcatctg ccagccgcca ctgccgtcgg
     1681 gttccgggtt gaacgattcc tccgcttcgc acagcgaacc gtcaggaaga acccagggct
     1741 tccattttcc gccgaagcgc gcttcaaact cgcgcgttct ctcgggatcg gataccgggg
     1801 aaagacttcg agacagcgcc accacgtcca gatgaaatcg ctcgatggcc cagctttcgg
     1861 gttcacagag ctgctgtccc cagtcgtaaa ggcgcgtggt tcctccccga atccccagaa
     1921 aagccttcag acggttgtac gcgatcgcca tgatgttggt cgaacccatt ccgcccagat
     1981 cgatggggac tctgtcgggt tcgcggtgag aaagtgtgtg ccggacgcgt tcgcgaggcg
     2041 tcatagcttg gtgtctccct gttgtgaaag gccaatgcac gcaggtctag tgaaattacg
     2101 accggtctcg aacggacggg aaggctccag cagggatatc tgcgaaaaac cccgtgttca
     2161 ggcgggtgcg acgtcttcac tcttgagcgg ccgtgtcgta tcttcagaaa acaccgacgg
     2221 gaacaaagga ttcgcccccg ggcgcaagtc gggcggttga tgctttttcg gaaaccgcgc
     2281 cgcgattgtc cttctcgccg cctgccggtg cccgcgagcg caaggctcgc gtttctggta
     2341 tacgaggagt gaagaactgt gggtttgacg ggatccttcc gaggcctcag catcacggag
     2401 atgcttcagc tcatccggat gtcaaaaaaa acgggtgcat tgcgaatcac gctcgtcgac
     2461 aacacgcgac acacaatcta cttccgcgcc ggacaggtga taggcgtcga atgtggaagg
     2521 gacagtctga taccattctt gctcgaacgg ggtctcctct cgaatgacga tctgaacgcc
     2581 gcaatatcgc agcagaaatc ttcttctcga agagcgcctc tcgagcagat tcttctggac
     2641 tcgggggcag tgactaggga cgacctggtt caggcgctca cctcgcatgc ggaagaaacc
     2701 attggggcgc tgattcagga aggtggcggc accatttcgt tcgaggacgg cgcacaaccg
     2761 caactcaaac tggaaattgc gctcgacatt cacaacctgt tgatggcggc ctcggtcaag
     2821 gcggacgagg cgctgcggga tcggacgctt ccctatgtgc cgactgacgt ctatgcgcac
     2881 tcaggtcagg gaaaaaccgc gttcgctgag aaaacgatgc ggttatgggt agaccattgg
     2941 cgcgtgtttc tggcgatcga cgggaatcgt accctgcgtg ccatatcgga aaagattcag
     3001 gtggcgccgg atgagttgat gaaaggcatt gccgcgctgc tgactctggg gtatatcgaa
     3061 ccggttgccg gagggcgcgc tcgccggatc gcactggtgg ttgacgaaag cgccatggtg
     3121 cagaagcttg tccagatggc actcgcggaa gaggacatgg aaatcgagag cgtgagtgat
     3181 gcagactccg cacaccgcgt cctcgcggaa agaacgcccg atgtcatcct catgaacgtt
     3241 gcgttgccag atacgagcgg ctaccggttg tgccgacaat tgcgcggcgg ggattcaccc
     3301 tgcaacggtg tgccgatcat tctgctcagc gctcgagata cgccacagga tgagaaactc
     3361 gcgatccacg cgggggcaac agccttcgtc tccctgccgt tcgcaccgga atcacttcgc
     3421 aacacggtaa ggagtgtgct ttcgtgaagt tccggacgga ggttgttttt cgcgccaccg
     3481 ggttggttgt tgctacggag tcgatcgctg cgcgttgaca gaaggaggtc accatggctc
     3541 tcccggacag tccacgcaac cgggaactgg aagcgtggct aagggacgtg accgcgggca
     3601 tcgcaactac gatcctggcg gaagtgtctg aagaccgaat tctggcggaa atggaatcaa
     3661 aggccggagt gagcgatgag accgtcgttc gctggggacc gggcgtgcag gggcaattga
     3721 ccggtaccgg taaggagtcc ctcaacggcg gcaacgcgtc ggcggatggt accgatcggt
     3781 atcggttccg gcgccccgtt tcggaagatc aggggcgggc tgtcctcctt tttctcgcgt
     3841 cgctgttcgg gttgtccgat ccatcagaac agggcgacga gtaaaccgtg tccggaaacc
     3901 gtttggggag ggttacgtct ctgccccttt tgtattgtgg cgcaataact tacggtcttc
     3961 agttttttcg aatgtgaacg ccgatttttc ccagtatccg agtgtttggc tgcccgagga
     4021 accgagaaat gccagaagaa cggcgcctgc ttgttgtcga tgatgaagaa gtgattacat
     4081 gggccatatc ccaggcgctc actcgcccgg gagagatgcg ggtggtgacc gcccgcagtg
     4141 gagaagaagc gctggaattg atggcgaaag agccgtttga tctggtcgtc acagacatcc
     4201 gcatgaaagg catgacaggt ttcgaactgc tttcgagaat ccgcgagacc tatcccgaat
     4261 ccggtgtgat cgtgatgacg gcttacggct caccggaggc gaaacgagag gcgacggaac
     4321 ggggatctct tttttacctc gacaagacgt tcgaactgga ggaattccgg agtgtcgtgc
     4381 ggaaggctct ggaacaggtg gaccgcgggc gggctgccgt tggtaagggg gaacaggagg
     4441 gtttcagcgg cctcatcggc aatctgaagc tgatcgacat agtccagctc cattgccttg
     4501 cgcgaaacac ggcgatggtg gaggtgcgtg caggaaaaat cacggggcgc gtgggattcc
     4561 tcgacggtga aattgtgttc gccgaaacaa gccccgggga aaaagggcgg acagctttcc
     4621 tcgacataat gagttgggat accggggaat tcgcaatcat cgacgaagcg cccgcagagc
     4681 agaacatcga tgaaagctgg gagtccttgc ttatcgagac caccgcactc atggaaaaca
     4741 gcaccggtga gcccgatgtc cctgctcccg caggagccga ttcggcagaa ttcgaaacgc
     4801 ctgcggatat tcacaccatc gtcgaagacc tgatccacac ggatggcatc gtcggcgccg
     4861 tagttgccgg cgacaccgga attgtggttg acagcgcgtt cgacggttgg gatggcgatg
     4921 acaacggaat tgccgaactg gccagttgca tggcgggaat ctcccgcgta cggttcgtcg
     4981 ttgagggtga gacacagcgg agccacgtca taatccagtt cgaccgcagg agagtcattg
     5041 cacaggaagt tggcgaactc ggcattttcc tgctggtaat cagttcgggt accggtggac
     5101 tcgcgcagca tctttccgcc acggacacag cagcaaaaaa gctggcgaga cttctttgaa
     5161 aaccattttg agtatttaac ttaacgagtg tgagttttgg ttcgagcgtg acgtgcggtg
     5221 cgggcgagga tcagcaatca acgtgccgca agaacgggct ccgttgactt acccccctcg
     5281 tttgtgtaca tctcttttcg taagaacgac gtcgaatcac gccccatctc agcggtgtga
     5341 ggggcaacat ggcaaccggg ttatcgcatt gaatctggcg ttccggagga gttcccatga
     5401 aaaaggtttt gcaggagctt ggtgaagttg aaggtattgt ggggagtctc atcgtggacg
     5461 ccgacgggat cccgatcgtg gaatgcctcg acgcggacag cgaactcgtg gcggcaacat
     5521 gcgttcagat agtcaattcg gttcgtgacg ccctggccaa actcgaagaa gacagactcg
     5581 tgcaggcgct catagaacct gctgagggaa aaatattcct ccgcgagatt ccggacgtcg
     5641 gaacactcgt ggcggtgtct gaaaagaacg tcaacgtggg gttgatccgg ctggctcttt
     5701 caagcgcgag cgaaaagatt cgcgcataca gggaggcact ctgatccgca gatgtgcgaa
     5761 aaaaggctgt ttcgtggcca cctccggtgg gaaccatccg ctccttcagg agccggcccg
     5821 aagcgttgtg aagatgatga atcggaatgc cgggggtcat gaatgccgac catagattac
     5881 gcaaacaaag agattagttg caaaatcgtg tactacggtc ccggcttgtg cggcaagaca
     5941 accaatctcc agttgatcca ccgcgacgtc ccgggaataa acaaaacgaa cctggtctct
     6001 ctggcaactg agaaagaccg gaccctcttt ttcgatttcc tgcctgtaac ccttggcgaa
     6061 atcaaaggct tcaagatcag gttccagttg tacacggtac cgggtcaggt gtactataat
     6121 gccacacgga aactcgtcct gagcggcgtg gacgggattg tgtatgtggt ggattcgcaa
     6181 aggagcaagc tgcaggaaaa cctggatatg atgcagaatc tccgcgagaa tcttgcggaa
     6241 taccgcatgg atctcgccac gatacccatg gttctgcaat acaacaaacg tgacctgccc
     6301 ggcatcgttc ccgtggaaga gcttcagcga gcgatgaacc ctcgtggtct tccgtggttt
     6361 gaggcagtgg ccgtgcaggg tatcggagtc aaagagacgc tgaaggctat cgcaacgctt
     6421 acgttatcac ggatcggcaa aagaacccag gctgcgcctc gagatggcgc tgcggcgggt
     6481 ccctccaacg ctaccgacac gggtcaacca aaacgctcga tattccggaa aacgaaccca
     6541 tgaaaatcca ctccagcacc gttcttcgtt tggcatcaat gcgtttggac gcaaggattt
     6601 cctggcctgt gagcacgctg aggcattagg gaggagacac tccatgggtg gaggtggggt
     6661 agtcatttat gacgagcaac ttacccggat caactcgttg atcgggcatc tgctcaaaga
     6721 gtccgacgcg aaatgcgcac ttcttgtggg caaggacggc agaacgatcg ccagacaggg
     6781 cttcacaaga accatcgaca cagaccagct tggggcactc gtggccggtg cattttcggc
     6841 ttcgaaagca gtggcggaat tgatcggcga acccgagttc tccactcttt tccaccaggg
     6901 gaaacacgaa agcatccaga ttcacctgtt ggacccccgg acacttctgg tcgtcacctt
     6961 cgatgaaaga acgactgtag ggatggtaag gctggtgacc aaagccgtga gcggcagtct
     7021 gcgggagata ctgcaggccg tgtacgacaa tcctgatgtg gaagagatcg gtacgcccga
     7081 ggaaatcgca agcacgttcg atggagtttt cggagattcg gaaccaaagg gtgggtcgaa
     7141 acagccctag tacgtgcctg accgaagggt tttgccccgg gaaattacca tgagacattc
     7201 ggagcacctc agaacgccgc cctgccatcc cagactgcgt gtccttttcg atctcctgct
     7261 cttctgtctt gtgatcttcg tgacggcgcc tgattccgcg gtccacgccg cgtgggcggt
     7321 gtacggagac agttggagct ccgccacgca ggcccaggaa caggttacgc gcgtcaaccg
     7381 ggttctcggg gagcagcagg ccagctatga acgcgcgacg gtccaggggc gaacgtactg
     7441 gcgcgtccga atcggcgtct atgcgacgcg aaacgaggcg ctcgaagcca gaaggagagc
     7501 aataaccgcc ggagttaagg aaccctggat gcgggaagta tccgacgcgg aacttgcggt
     7561 tcagacgcca tctcccaccc cttcgcaaac cgcgtacgcc gactcagtcc gcgcggactc
     7621 gcttcgaaag gtgttcatgg attcgctggc gagagccaca aggcgaagga tcgacagcct
     7681 cggcaccgct gcgcagcgcc agacaatgga catcatcgag cagatgcggc tccagatcac
     7741 tcaggaactg gtggaacgaa ttgagcgcga gcaggcacgc gaccgctcct cgtacgttac
     7801 cacggccgaa caatcgcaga tgaccaatgc ccttattgac gaaatgagca gccgtttcga
     7861 ggtgctcagg gacagccttc ggacagaccg ggcgatgaga gatgcccgag acgccctgtt
     7921 gccccgcata agcggttacg ttgccgccac ggggatcgct tcacacgatc gcccggccgg
     7981 ggcgggcatc agtacgagcg cagaggtcgc cattccgcac gctcaggctc tgttcgagtg
     8041 ggcgaacgac agaggcgggg tggtgcttga tgtgcgcacg gcgggcgctc tcgccgtgga
     8101 gttgcatcag gcgttcgtca cctggtcctt cggggccact gggggcgcga gtcgctctct
     8161 ggggcttggt ctctgtgaaa tgccttacgg gctcgagccg tcgttcacgt cagacctgct
     8221 gacgtgctcg cccagccagg taagcgccgc catccctccg cctgcccgcg ggcttctcct
     8281 cgttccgcac gacggggagc gagttacact gtcggttttc ccgttcggcc ggtgggacga
     8341 agggacacag aaggggttgg tccaactggc gctgcacaac gaggccacga ggttcgaact
     8401 caccgccggc ggggaacggg tgcgttacca gaacgaagtt gctccgaggc ggtacgagtt
     8461 cgccggcatg gccaatgcgg tgatcgaacg caagggcgca aactggctgt tgggcgcgga
     8521 agcgaatctg gagcggcgac gtcaaccggt atccggaatc ctggcagtga accgcaggca
     8581 ggtggacaca tggggcggac ttgcactggt gcaccggcgt ttcagcgaaa gatggggatg
     8641 gacgctgcgg ggggacttca ccacccaggt aacgacatgg acgaatctgg ccgtgacggc
     8701 acctccaaga gaaccattgg ctcggaactg gacggtcacg agcggtccca tctttttcct
     8761 gggggacagg cttcgcgcgc gggtcaacta cacgtttgat cttcaggaag agcgggcagt
     8821 gggaacgtac gcccttgaac gaagccatca ccatcgcttc gagttcgggc tgagccacgt
     8881 gttctgaccc gcacaatgaa ccgcaggtgc acaacagact tcgccaatcg cacgcgtcgt
     8941 tcatccttcc cgcgggccgc tatgttgacc gtgcccctgt atgctgctat ctttttcgca
     9001 tgttctggca ccggtgcgaa catttggccg gttgcccgtc tcaacccatc acagacgagg
     9061 tgctcacatc gttctaatgc ggaagcccaa accgatggct ccggtacaga aaaatgagga
     9121 ccgcacacgg gtgaaccagc atatcagagt ccccgaggta cggcttgtcg gagctgacgg
     9181 cgaacagatc ggaattgtca acactcgtga ggcgctccga atggcggagg agcaggggta
     9241 tgatctcgtg gaggtcgccc cgaacgcgtt acctccagtg tgccgcctca tggattacgg
     9301 gaaattccga tacgagcaac agaagaaaac ccgggcgatt acgaagaacc ggggcgggga
     9361 acagaaggaa atccagatga ggccgaacat cgatctgaac gattatcaga cgaagttgcg
     9421 gcacgcaaag gagtttctgg caggtggcga cagggtcaag ctgaccgtgc gattccgcgg
     9481 tcgagaactc gctcaccgcg acatcgggcg ggatcttctt gagcggttcg cgaaagacct
     9541 cgcgggagac ggccggattg aagggggcgt tttcgaggaa ggtcgtaata cgagtcttaa
     9601 cgtggttcct tctcgatccg gtggcagcgg agcggctccc gcttcagctt ctcccgcttg
     9661 aaaagttctt gacgccgact cgataaacga gaaacaaccg agccatccgg gtatcgtacg
     9721 gatggctcgt tttctttcgc ttcgtcagcg caggattcac gccatgtgaa cttcgacgtt
     9781 gtcggcaaca gcacccagta ttgtgaagtc cgtccggcac gtctgaagta tcgtcgcagg
     9841 gaccagcgtg ttgacgcgcc cgtgcgatgc aaaccgggcg atgaatcttt gccagctatc
     9901 cttcccgaat gctccatcca accagaaact tcggtgcttg gctcgtacaa tctgagcggg
     9961 accgatggtg ttcgccttcg gaggaaccca ggaccaatcg cccccgaagc tgtggagcgc
    10021 gttctgcatg atagtcattg gatggagttc caccagcctt ggaccggcct tcatgtacgc
    10081 atcgaggtcg ttaccaaact caaaaccgag atgtggctcc cagaaggcga tgtgaccaca
    10141 ccagcctaca ccgccgtaca ccacgtccac ttccccttcg tcttcaatca tcttcccgta
    10201 gttcttgata accgctttcg tgggaaagtg aatgttcttc tcgctcgggc gaaggttctt
    10261 gtggattcgg ccgtagaaac tttccatcat cgcacgctgg aaagatcctt cccagtccgc
    10321 gggcgcggtg ttcccctcgg cgtcggcgta ttcgtccatg ttgaaagaaa cgacatgatc
    10381 cagtgatagc cgcatcgagt taatgatttc tgccgcaatg ggatactggc ccataggacc
    10441 cacaggaaaa atcgcgacaa actttcttcc ctcgtcgcgg gccttcttga ttcgctgcac
    10501 gatatcaaga gcgaacgcga gattgagatc cgcgatgttt tcgatgattc ggatgcggaa
    10561 ttccgggttc gggtgtctgg tcaggtcctt ttttttgatg gcccgaacgc gcgcacattc
    10621 cgccgcgtcc tgaaaaggaa tgaacttggc gaggctcaac gtgaaggctg acatgacgca
    10681 ctcctttcgt ttttgaaggc tgcaccgtat gccggaaccg cattcgaaag gatagccaac
    10741 gcgccggaca ggaaaaagag gcaccgggtt gttttgctcc tccggcgggc cgtccgtaag
    10801 ttatgtatac tctacggtct gaacgaaggc atagttttcg cggggtagga tcaagcatgt
    10861 ctgcgctctt gctggcaagc gttctcagtt acctcctggg tgcatttccc accgcgatca
    10921 tcgtcggcaa gttgttgcgc gggattgatg tccgcgacta cggcagtcgt aatgcggggg
    10981 caacgaatgt ctggcgcgtg ctcggtgcgg gacctgcact tttcgttctg gcaattgacg
    11041 ccctgaaagg tgcgggggcc gtgcttctga tagccaggat cgccagcgat tgttgcccgt
    11101 gggatcccct gacaacacag gtcatctgcg gcatgatggc ggtggttggc cacatctgga
    11161 cggtgttcgc ggggtttcgc gggggaaaag gcgttggaac cggtgcggga gtgttcgcgg
    11221 cgttggcccc atggtgcgtc ctgatcgcaa cggtttcgtt tgtggctgtg gtggctctga
    11281 caaggtacat ctccgctggg tctatcactg ctgcgttcgt gctggcagcg ggtctggtta
    11341 ttcagcgtct gttcttccct gaatccctgc ccataccggt agttgtgatc gggtgtgttg
    11401 tcgcggcggt ggtaatcgtc aaacaccgca cgaacatcca gcgccttatc aacggcaccg
    11461 agaatcgttt cggcaaaccc gcgtcagcaa ctcttgcgga aaaagagggg caccgttcgt
    11521 gaatgttgtc gtgatgcggg ccggcagctg gggaagcgtc ctgtcggggc ttctggcaag
    11581 aaatgggcac agagtcagcc tgatcgcgcc gcatgaggct ttgcgcacgg aacttctgac
    11641 gtaccgcgaa aaccgaaagg cgctccccgg cgtcccgatt cctgacacag tggcaattct
    11701 ttccgcaccg gacgatgtgc tggaaagtgc cgacatggtg gtgtttcccg gtccgtgtga
    11761 attgatgcgc gatatggcgc gttcagtgaa atctgccaac tggcagggca aactggtcgt
    11821 cacggcagcc aagggcatcg agcagggttc tttcaagcgt atgtccgaac ttctcgcgga
    11881 ggaacttccc gaagtgcctc cggacgaaat cgtggcgctt tccggtccga gtttcgcgcg
    11941 atttgtcggc ctgggtcacc cgacgacggt tgttgcggcg gcacggtcgg agaaagctgc
    12001 ggtagcggct cagcgggcat tcatgagcag cacattccgt gtgtattgct cacccgattc
    12061 cctcggcgtg gaactcggcg gagcactgaa aaacgtgatc gcaatcgccg ccggtatgtc
    12121 ggacgggctc gggtttgggg acaacacccg cgccgcattg atgacacgag ggctcgccga
    12181 aatcacccgg ctcggactgg ctatgggcgc gcagctaccc accttcctcg gcctatccgg
    12241 cgtaggagac ctcgtgctta cgtgttccag ccgggagagc cgaaaccatt tcgtcgggga
    12301 acaggtcgga aagggccgga cgctgaaaga ggtgctccaa ggcatggtta ccgtcgcaga
    12361 aggagtgaag accgcccccg ctgcgatcgg cctggcggag aaatggggcg tgccgatgcc
    12421 gatagcgagt gaggtcaacg cggttctttt cgaagaaaag aaaccacagg atgcggtcac
    12481 tgcgctgatg atgcgtgacc cgaaacccga acactggagc tgaatccatc cgcgggacgg
    12541 ttgttcgtcc tgcaatccga ggtgaaaatg ctcaaacgca gattcacgcg cctgtactgg
    12601 cgcattggtg aagtcgcaga aatcaccgga atttcccagc agacgttgcg agcctgggag
    12661 caggaactgg ccatattgcg tccgcggcgc gatgcgagcg gcgtacgggt gtaccgggag
    12721 cgagatttcc gcatcgttct cgttctgcaa cagctcatcg agaaggaaaa gtggtccgtt
    12781 tccgacgtga aagagcttct ggtggccgcg cccgacgaaa ttcgggccat gatcaacgcg
    12841 gtgcccccgg aagcgctgga ggggacaccc gatgagaatg aaccgtccac aacagcgcgc
    12901 acagttgaaa aaccggaggt cgctgcaact cctcatgagg acaagaacga agaaccggct
    12961 caggcgggcg ttcggcttgc aggagccgcg gtgccgcggg aaaccgaaca agaagtgacg
    13021 gaggctccgg ctgaagaacc gggctccggc ggtggtgaag ttgtcgagac tcccgcggtt
    13081 ggtgccgcag catacccacc tgcggaaacg gaagaacgcg aactcgcggg cgcttcttca
    13141 ccaacgcttc cgctcaccga agaaccatcg accgttgtcg acgccgccga agcggaagag
    13201 acggccgaac cggcgtcggg cgagatgtcc gccgaagagc gctcttttcc cgccgaaggt
    13261 gtgtcggaaa ctgaagagac atggcaggac accggactcc ctccctttgc tacggagact
    13321 ccggccgaac ctgaacggga acccgtggag gctcctacct ccacagagcc tgtcgttgag
    13381 acttcagacg gagacatgga tctcgccgcg tccgcgccgc caattgaacg cgaggaattg
    13441 gcgcccgtgg aggtccaaga tgcgacacca agcgttccgg ttgaagccaa tctgatggag
    13501 caaccggtgt tgccggggga ccaaatcgcc acggctgagg atgtgtcacc agctaccgag
    13561 acgggttacc acccggaaga agaggatcgc gagcaggttt tcgccgcggc ggaacagatc
    13621 gagtcattcg tccacagcga aggaatggcc gaagagacag cgggcgggcc ggcggctgga
    13681 gaggcgacgg aagaaatcgg catggcggcg ccggtttgga cgccggtgga agaagagggc
    13741 gaggcctctt tgcagggaga gccggaagcg gcgacgcccc gcgaagcttc ttcgccggat
    13801 tccgagttcg aaccggagcc ggagctcgcg gaatcggggg agaaagagca cctgaatcag
    13861 ccggtcgttc ccgaggttgc tgccgaagaa agcgaaccat cccccgctgt gcaaccatgg
    13921 gacggggcac ctgtgaccga cgttgccgaa acgcctccct ccacgtcgat tccggaaccc
    13981 gaaccactcc cctcagcaac tccgacaatc ggagaagccg ggagcgagat cctggcggct
    14041 gccgaaacgg cagcgcagat cgcggagagc gtttctttgg aagcgccaga aaacgaaggt
    14101 gctgatggga cgcgggattc cattgccaat atggcgttcc cgcaggagca gcctgaatcc
    14161 gcgccggaaa cgggtgctcc ttctgatgcg aatgtggcct cggaaacaga accgggcgag
    14221 accgacaccg aggaagaaga ggcaatctca cccctcctcg ccatgccggc ggacacacaa
    14281 accgagcttt ccacccacga ggagatcgag ccgccgcgtc cggaaaggcg ggaggttttc
    14341 gagctcatcg cgtccgaggc ggtgccagtc ccggagactc ttccaaaatc tgtggtcgag
    14401 gacctgcgcg cgttacgtga ggaactggca gccattgccg agatggtgag aatgctggtt
    14461 gagccgtctc ccgaaagcac cgaggcaacg aaggatgtca gtgacgaacg ggcactcgct
    14521 gagtgctcat ccggagatga gaagtcgtct gatgcgtagc gaggtttgag gggggttgaa
    14581 tggagaggaa atccgtgaag tcctcaccac ggattgcccg ggacgcatct cgcgcagcgg
    14641 aatcgtcacc cgctcagttg ccggggctcc gcctcctgag ttcgtttctg cagtacatca
    14701 cggctgagcg gggcctttcg cagaataccc aggctgccta ccgacgggac ctggaccgtt
    14761 acgtgcgttt tctggaaacg aaacgcgggg gaatgactcc ccgaacagca accagggagg
    14821 acgtgtcgtc ttttttctca tggttgcggg gccagaacct cgcgccgagc agtatcgctc
    14881 gaacgatgtc cgccgtgcgc gtttttcaca ggtttctgca gaacgaaggt gtggcggaat
    14941 ccaacccgac cggcaccctt gatacgccaa aactcgagcg aaatcttccc gaggttctca
    15001 gtcgaaacga gatcgagcgt cttctgaacg cacctgaccc ggttactcca cttgggcttc
    15061 gcgaccgtgc tatgctggaa tgcgcgtacg cgaccggtct ccgcgtaagt gagctcattt
    15121 ccatcgacat acgcaacctg tttttcaacg aaggcttcat ccgcgtaacc ggaaaaggga
    15181 acaaggaacg tcttgtcccc atcggacgaa aggcggtaga gtgcatcagc gcttatgttg
    15241 ctacggcgcg cagcgcagtt gcaaaaccag agtcgggcaa cgtactcttt ttgaactgga
    15301 gaggcaggcc tttgacgagg atggccttct ggcagatgct caagaattac tgccgtaaga
    15361 ccggtatcac gaaaaccgtc agcccgcaca cgttgcgaca ttcgttcgcc acgcacctgc
    15421 ttgagggcgg agcagacctc cgggcagtcc aggaaatgct tggacacagt tccatcgcaa
    15481 ccacccagat atacacgcac gtagaccggg agcatctgaa ggcaatgcac cgccaattcc
    15541 atccgcgtgg ttgaaaccac ggcgcgcggt tatgggctcc gcatatcgta accggcgtca
    15601 aaacggagtg taatatgcga gaatcgtttt caatccctct gataactctc ttcggttttc
    15661 tccttctcgc gtgcgcgact gaaacctacg ccgccggcac cctcggccag cttgacgaac
    15721 agcgactgag gcagacagtg gattcgctgt acgggtctcc gtatcgatac ggcggcagat
    15781 cgcgggaagg ttttgattgt tccggttttg tagggacggt gttccaagac cagggcatag
    15841 aacttccgcg cagctcgaga gagatgtacc gggtgggaga tccggtcaaa agaaaccagc
    15901 tcgcggtcgg cgatctggtt tttttcagaa cgcgtggaag aaggcgaggg gtcagccacg
    15961 tgggtatcgt caacgggccg gattcattcg tgcacgcgaa cacgagacga ggcgtcatca
    16021 cggagagact gacggacccg tactggaaag cgcgttacgt cggcgcacga cgggtggcta
    16081 ctcctgtcag tccgcctccc gctcaggaaa ttgccgaaga cagctatccg ttcaacacct
    16141 cctcgctcgt caataccccc acgatcgcgc tctgcccggg gaaaggactt tctgcgctga
    16201 gttttgcaac cggctttcgc ggggatgtgg ccatcagtcc ggagttcacc ttcatgaacc
    16261 gccttcaggc aggcgcttct gttcgcctcc gaagagcggt agggacgggt acgccttcga
    16321 ttgcggtgcc aagtatcaga gcgaagctcc gcatcaacga acagatcggg cagatacccg
    16381 gtttcgcgat cgggtttgac tcggagagag acgaactcac ctattccacg gcgttgggtg
    16441 attccacagc ttcctcgccc cgccgggggt tgttcgcggt gggaagcgga acacttgtct
    16501 accccaggag ctttttcctg gggcgtggaa ccgggcacgc aggtgcaagc ctttcatcat
    16561 tcgccggcaa cggcatcaaa cggaaccttt cctttttcat gggggtagat cagcagcttc
    16621 tccagcgcat gacgctcctc ggggaaattg acaacatcat gggcgtgggc ggttggcgag
    16681 cgaacgtcgg cgcccgtttc agcatcactg atgatgcagt actcgaatac gcggtgctcc
    16741 acatggccgg agacaacatc cgccccgaca aagtcctcaa gttcagtttc agcgtgccct
    16801 actgatcgtt tccccgtcac ctctgttttt ctgccttttg cgcgcaactc ggacgcactt
    16861 tgcccgtcaa cgggatcgta catcaatgga agtcgcgact tttcaccacc tccggcctgg
    16921 gagtgacacg cggcacccat aaccggcgtg caatgaggtg cgtaatacca ctcctcgatt
    16981 gaatctctcc cgtaacaccc agaaatgacg tagtcttggc aagaattcga tgccgctggt
    17041 agatgtcctt ccagatgatc aggttcgcgt ggcctgtctc atcttcgagc gtcataaagg
    17101 tcacaccgtt ggcatttccc ggacgttgcc tgcagatgac gagaccggca tacctgacca
    17161 gagaacgatc ttgaagcgcg cggatttcgg aggcactcag tatcccctga gcggagagca
    17221 aaggcctgag cgccagcagg ggatgtgccc gtgtgctgtg cccggacgct cggtaatccc
    17281 acgcggtctt gtccgcttca gacagaagct caaataccgg ttccgggtcg ggttctgcca
    17341 cggcaagagg caatgtgtcg gtacgtgaag ctcccttgac acgccacact gcctgacgcc
    17401 ggtgccgttc gagactctca aacccgcctg cctgtgcgag agaacgcaga acgccctcgt
    17461 ccagactggt gcgccggaca aaatcatcga tatcagagaa agggccgttg atacgcgcat
    17521 tctgaatcgt ttctgcatcc ttcttcccga gcccgttcac gtgcctgagc cccattcgaa
    17581 ctgcaaattc cccatcaggc aagggttcca gcgtgcaatc ccatgagctc cacgacacat
    17641 ccacaggcct gacggacaca ccgtgccgta tggcgtcctg aaccagtgtt gcagggctgt
    17701 agaaacccat cggttgcgcg ttgaggatcg agcacaggaa ctccgcggga tagtggcacc
    17761 tcaaccatgc agacgcgtag gaaatcagcg cgaaacttgc actgtgggat tccggaaaac
    17821 cgtattcacc aaatcccttt atctgctcga aaacccgctc ggcaaatgct ttgtcgatcc
    17881 ccttttctct catccggttc acaagacgat cgtggtgccg ttccagcccg ccctttttct
    17941 tccacgcggc catgtcgcgc cgcaactggt ccgcctcgcc gggggaatag tcagccgcca
    18001 ccattgcaag tttcatcacc tgttcctgga acagagggat gccgagcgtc ctttgaagca
    18061 cgggttcaag gcagggatga gggtagataa ctgcctcttc gccattgcgg cggcgcaggt
    18121 acgggtgcac catccctccg gtaatcggac ccggccgaat gatggcaatt tcgatcacga
    18181 gatcgtagta gttccgggga cgcagacgtg gaagcatcgc ctgttgcgca cgactttcta
    18241 tctggaacac gccgacagta tcaccccggg cgatcatttc gaaggttgac cgatcatccg
    18301 ccggtatcgt cgccatggag agattcacgc cccggtggga tgccagcaga cgaaaacaga
    18361 tatccagttg tgtcagagct ccgagcccga gaagatcgag tttgaacagg cccagcgcct
    18421 ccacatcgtc cttatcccat tgaattacgg tgcgcttttc catagtagcg ttttcgaccg
    18481 gaacaatgtc gagaatctcc tccgccccga gcaggaagcc accgggatga atggacagat
    18541 gccggggcat tccgatgatt tcattggaga tagtcagaag gtgccggtga agtggctgat
    18601 cgggatccaa accggcgtca cgcagggatg tgtcggtgag gtcgccgtac gatgacgtaa
    18661 gttttgccag acggtcacac gcactctccg ccattccgag cactttgccc acttcccgga
    18721 tcgcccgttt tgcccggtat cgtactacgt ttgctaccat ggccgctctg gatcgcccgt
    18781 attcgtcata aacgtgttga ataacctctt ctctgcgctc gtgcataatg tcgagatcga
    18841 tgtcaggcgg ctcggcacgc tcgcggctga tgaaccgttc gaaaagaagc cccatacgca
    18901 ccgggtctat cgcagtaata ccgagacaga agcagaccgc ggaattcgcg gctgacccgc
    18961 gcccctggca gagaattcct tcgcgcctgc aataccgaac aatctcccac atcgttacaa
    19021 agtagccggt gtatttgagt tccgtgataa ggctcagttc tttctccact tgcagggtga
    19081 cttcatcagg aatgccgtcg ggataacgct gggctgcccc ttcatacgtg atttttctca
    19141 accactcttc gcccgtcata ccgttgggca attggcctga agggtaacga taggcaatct
    19201 cgcgcatggt gaatgtgcag gaatcagcca cggcgcatgt tcgttcaagg gattcgagat
    19261 cgtcgcggta gaggcgcttc attcgcgctg gtgatttcag gtcgtactcg gcgttcggct
    19321 tcagccgcct tcctgctgag gaaagcgtca ctccatgccg gatacacgtc attacatcct
    19381 gcaacgggcg gcgcgacact tcgtggtaaa gcacttccgg taccgcaagc gtcggcaggt
    19441 cgtacttccg cgcacgaaga cgcagaagcg cttcctttgc aacgtcatca gccctccgat
    19501 ggcgggagat gatcacatgg aggtggtcat gaaaggcttc tttgagttgg cgggctacga
    19561 tatcgggatc cgccttgcgc aggagcaggc ttcgatctcc accccagaga gccagaaggc
    19621 cttccgaatg cgagcagacc tcctcccatg aaaccttgct ctcacctttt ggcgagcgca
    19681 tcctccccgc gctgatgagt cgacagaggt tcgagtagcc tttccttgtg cggcagagaa
    19741 gaatgatcgt tgaaccgtca tccagcgaaa tctcagaccc gattatgagc cgtgctccag
    19801 tggattctgc tgccagaaga gcctgtaccg cgccatatac accgtccctg tcggtgagag
    19861 caatcgcatg caatccgagt gttgcagcct gctctaccag ttccgatgga tgcgacgccc
    19921 cctcaaggaa cgagaaattg cttttgcacc agagagctgc atacgacact attcaacctc
    19981 tccctggatg aaccagtcgg cgcgggaata gtcataatag acccacagca ggcgcccttc
    20041 gtttgtcagt atgaaccggt actcccgcac ggtctttctc gaccaccagt cgccagaaat
    20101 acggtattct cccaccacgc gattacccgt tttacggtat tcatcgggaa ggcgctgcgg
    20161 cactgtgtag atacgccgca tgtaaggaaa ttcctcggat acgtcgcaag gattcggaac
    20221 ggggacggag cgggcgatgt cccagtgaaa cgagcgttcc ggcaggtgtt catcggcgag
    20281 atgagctgtt accacggcat catcgccgaa cgccgcgcga atacgtgcaa gcgctcgttc
    20341 tccggctacg cggtttcgag gctccggcac gtcgaacaga tccctctttg ccgtgtaggc
    20401 aggtgatgac tgaaccgaga gaacgatctc ctcaactgcc gagggaggag gcccggcttc
    20461 gatacggaga tgaataagct ccataagccg ctggatgttg cctgtggggt tactcggcgt
    20521 gacttccgac gaatacggca ggccggtatc aagtgtgcag gtccagtgga tactccctat
    20581 gagttctcct tttttctcca cttcgcctgc gagtgtcgta agcatcttct ccagaaaccg
    20641 acaaagccgc tgagtgtcac gttccggtgt atcgaatatc atacgccgcg catggagcgg
    20701 tttttccgca tctgcctgaa gaggctgcca gaggtctcca cacgcgcttt tgtacaggca
    20761 gaaaacctgc tcgccatacc gcaagcgcaa gccgtacaca ggcagatgga gcagattgtc
    20821 tacggtgttt acgccgagca ggctgagttc ctgagtagtg tcaggaggta gccccagcgc
    20881 gtgaagcgga acggcgagcg ccgcctttcg ctcgagttcc ggcgaaggga aaacactctc
    20941 ccctttccgc gttttcgccg cagcgtacgt accaaaccga gtaaacccca ccgcgattga
    21001 gctgacaaat ccctccgagc ggacagcctt ccacacttcc ttcgaccatg cggtttccga
    21061 gccaaacagc ccggtgagac cgcctgcgtt gaggtgaaac acacccggct ccccggcgtc
    21121 cggctccacg tccggggtaa acgttctcat gcgccgtgat aacagggcga tacaaccggc
    21181 gaggtcttcg gccgaaacgg taccggcatg aagctgcgga gccaacccga gagcggcggc
    21241 ataccgcatg ccggtccgaa cccccatctt tcgcgccacg gggttcatgt aaaggatttc
    21301 cgcctgcggg gcatcccgtg tgataacggc aacagggttg ccccaccact ctgaatgcct
    21361 gaggcaaagg atctggagag gcaaatccgc aatccatacg cacgcaagcc gggacatcgc
    21421 acaagacctt atcctgcgtc aggatgcttt acacagtgca attggagcgg taaatctgtt
    21481 atcgctacgc atgccaacgg gaacaccacg atattccctg tgaacccatc ccggcgcatg
    21541 ctgtttatcc ttgatcgctt caagactgat accggccgtt gcgtcgaatt ttcccacgcg
    21601 ccgcgcctgg gtgcggagag agataagcga gccgagggac gaagcatgca cgggtttgcg
    21661 cgtcaggcat acggttgcca cgtttttgac atgagaaagc cggacaatgc gggaaagaag
    21721 cggcatagga atgcataccg gcgatgcatc gctgagatca atcaccacaa ggccgaaccc
    21781 gccggagcgg ataagcatat cagcggcgcg aggggcatcg gatagttgag agaggcgcac
    21841 gacgataaga gcggcaaggt caaccccgta cgcagcggca tccagcggga agaagatatc
    21901 cgatgccttg cccacccatg ccgtcggctc accggcacac tgggcctggc gcacaagcat
    21961 catgacactg gaaagcgccg ccgggttttc ccatgcggag atttccgtca gtcgccctcg
    22021 caattctttg taccgccact cggacctgtc ggcgtgtatg tggagcgcag aagccgattt
    22081 aatgggaatt tccctgagca tatcctgcac tgctgagatc accacacacc taccgggaca
    22141 ggtttgaagc acacataata tacacgtaat aatacgggta ttacaggaaa tgtcaagggc
    22201 aggggagtgc cgggagctcg gtcccttcgt accacacaaa aagccgcacc tgagccatct
    22261 tcaggcgcgg ctttt
//
LOCUS       DAOSOO010000028        21806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2385_length_21806_cov_5.400441, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000028 DAOSOO010000000
VERSION     DAOSOO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 21806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21806
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_21806_cov_5.400441"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..752)
                     /locus_tag="PLT86_03575"
     CDS             complement(126..752)
                     /locus_tag="PLT86_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35728.1"
                     /translation="MDFSWLIIFIIISIASTLLSKRKEKQEAERRAREQAGKPPKPSP
                     AAGPPPVAPGPFGPLWKPAPPKPPSRPQPVPAMATDEEDAEKERPEPQPVVPARPLFK
                     EMQRELDERAAKTASVPSQEPVFVPEDWKREAQPRPVQKVSAADIDAHEGESFSRRTV
                     ADAAPSAPVHRAKRFGIGRGDLKRAVVMAEILDKPLALRDPVPRETQF"
     gene            complement(755..1780)
                     /gene="floA"
                     /locus_tag="PLT86_03580"
     CDS             complement(755..1780)
                     /gene="floA"
                     /locus_tag="PLT86_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703919.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOT35729.1"
                     /translation="MYDQTLLVAVGVFAVIVFLILFTYFIPVRLWIEAQFANVPVKIS
                     TLIGMRLRNVPPARIVLSLITSTKAGLNLQIDELEAHYLARGNVQLVVAALISANKAG
                     IELPFARAAAIDLAGRNVLEAVKMSITPKVIDCPDPSRGRSTIDAVAMDGIQLKAKAR
                     VTVRTNLDRLVGGAGEETIIARVGEGIVSAIGSASSHKQVLENPDVISKKVMEKGLDS
                     GTAFEILSIDIADVDVGDNIGAKLQSDQAEADKRIAQAKAEERRVMAVALEQEMKART
                     QEMQAKLVEAEAQIPLAMAEAFSKGHIGVMDYYRMKNILSDTEMRSAIAREGVSPQAG
                     DNPPARS"
     gene            complement(1854..2333)
                     /locus_tag="PLT86_03585"
     CDS             complement(1854..2333)
                     /locus_tag="PLT86_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT35730.1"
                     /translation="MVVTWAFVIALALLGFVLLMLELFVIPGFGITGIAAIAAILGAV
                     WYANLHLGTWQAVVVFCVTLLGSVLMISRTARTGGMKQLRNPSVSPGKVWDETKAREL
                     LPQVGARGVAVTKLRPAGIVQIGDRRYDVTVLGPIAEAGQAVEVVQIEGNHIKVKAL"
     gene            complement(2367..3827)
                     /locus_tag="PLT86_03590"
     CDS             complement(2367..3827)
                     /locus_tag="PLT86_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT35731.1"
                     /translation="MRRSAWRTTYFILIAGGLLVGASASGEAADSLRKATPAADSVSP
                     KAGLRDSLWQRLESEFPAGRMAGRGKTVAVVQVEGMIDGGLAAFIGRALDEAKAMRAA
                     AFVIEMNTYGGRVDAADEIRTALLNTDVRTITFIHNNAASAGALISYATDVIVMSPGS
                     AIGAATPVDQSGAVATNKVVSYFRSIMGETARAKGRDPRLAEAMVDSSIVVPGLENAP
                     RPLTLRTDQAIAFGVAQMQAASLAEALRYNGLEGAKMVRLETNWAEHIVRFLTHPVVS
                     GLLMTVGALGVIYELTSPGFGLPGIAGITCLVLFFGAHWIVRLAQIEELLLFAAGVAL
                     IVAELFVPGGILGIIGGALVLTGLFLSLVGRIDLVSPGDLTHAFTSIGMAVVLTFIGA
                     LVLIKSFSQLPMFRKLMLEKSQNPGTGQTASAAGPQPNLLGAVGVADSPLRPVGRVAI
                     GGKYYDAVTDGEHIENGASVKVVEVDGLRGLVVRRV"
     gene            3984..5924
                     /gene="metG"
                     /locus_tag="PLT86_03595"
     CDS             3984..5924
                     /gene="metG"
                     /locus_tag="PLT86_03595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT35732.1"
                     /translation="MDRFYITTPIYYVNGPPHLGHAYTTVVADALTRFARLEGKDAFF
                     LTGTDEHGEKIAQSAQSKGIAPQKWVDDVSAQFRKLWPELHIEPDDFIRTTEERHIRV
                     VQAILQKVFESGDIYYGEYGGNYCVGCERFLDDDELVDGKCPDHGVAPRFVNEANYFF
                     RMSGYQDWLISHIQENPEFIRPERYRNEILAFLRKPLKDLCISRPKNRLSWGIELPFD
                     SKFVTYVWFDALVNYVSALGWPDGERFAKYWPVAEHITAKDIIKPHGIYWPCMLKAAG
                     IPVYRHLSVHGFWRGADGRKMSKSLGNSVDPAEMAQRYGADVFRYALLREMPYGQDAN
                     IGEEIIATRQNSDLANDLGNLISRTLKLVFRSFGGVVPRVSETRVEDTALRDQWIGAL
                     TVVREAWHELRMSQGIETVIDCIRATNRYFDQMKPWELAKRGDTERLATVLVSALDSI
                     RIASVLLTPVMPERMAELRRQLGLTSSPSLEEAERWDGFAEGHRLAEGQSLFPRVDLE
                     EVRTRELHRREAATQSGTGDKPEPAPVESAAPVTIEEFRRVDLRVGTIISAEPVPGAR
                     KLLRLQVDIGEAAPRQLVAGLAEQFEPAHLLGRQVVVVANLEPAIIRGIESQGMILAA
                     DDQGRLALLGPAAAMPNGARIR"
     gene            5970..6956
                     /locus_tag="PLT86_03600"
     CDS             5970..6956
                     /locus_tag="PLT86_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOT35733.1"
                     /translation="MLDLYRWHLRARRMNNPGAIAVGFYFLFAPPTLLPAGDQVHTLT
                     GHGSPVMALACTRDSRYILSAGRGQVIQWNVRNGSRVFTLIHPGWALSIAVAPDGSQF
                     ATGSDDGLIRVWRLDNGGLLRTLEGHRGLVVALAYSDDGTTLASAGFDSVVAIWDVAG
                     TEAVRLLRGHAGEVNGVLFSATGDTVFTCSADGTTRLWNTHDGSLLQVFRGHSDSVRG
                     VAHSRRENILATCSNDSTVRLWDVKSGECVRVLRGHSDAVSAVAFSPDGRFLVSTGSD
                     RTVRVWRVDTGETERVLRGHTDWIRPLLITRDGNHIVTGASDATVMVWRRPW"
     gene            7089..>8032
                     /locus_tag="PLT86_03605"
     CDS             7089..>8032
                     /locus_tag="PLT86_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35734.1"
                     /translation="MTKLRLACVGAGPDKHSRSRNYLAVVKALHDLYELCAVVDPNES
                     NAREAAAEYGIPGVYTDMEKAIKETRPDVVLRLAPTDSAMAICVTAAQMGCNVISEIP
                     IDITLPRADATIKACRENNVRIEIAENVWLWPEEQLKQEIVRRGIIGRPVHARLKYPC
                     GSYHGFNGVRMILAEEAVRVLGFGGTVEVMPLPAYAGGMMTHVQWDGACVEFSSGLKL
                     LFEMPPKKPVWERNWDIIGTHGYLTGDSLVLYREGHHHGSGEEERFPIESVYREIQGQ
                     RVLQAVRVNTDPVVEWVNPYIRYGISGTDDIAKAAILE"
     assembly_gap    8033..8132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8264..8536
                     /locus_tag="PLT86_03610"
     CDS             8264..8536
                     /locus_tag="PLT86_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT35735.1"
                     /translation="MLDALGMVETKGLVGAIEAADAMVKAANVTLIGKETIGGGYVTV
                     LVRGDVGAVKAATEAGSAAAQRVGELVSVHVIPRPHAEVEKILPKA"
     gene            8552..9037
                     /locus_tag="PLT86_03615"
     CDS             8552..9037
                     /locus_tag="PLT86_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT35736.1"
                     /translation="MQTDSANLALGLLETRGYTGLIAATDAATKTAGVEVHSIARATG
                     GLVLVALVGDVASVQVAVAAGLERAQAVGSPISAHVIARPDDQVWKSIGFPSPADKGP
                     SPVAESAQPNTKEDDLDALPVRALRHMARSLPVGLTGRQISSASRDQILSAIRAARSH
                     K"
     gene            complement(9128..10108)
                     /locus_tag="PLT86_03620"
     CDS             complement(9128..10108)
                     /locus_tag="PLT86_03620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOT35737.1"
                     /translation="MPNRVLKLVAALTVAVSADHAGSTVLQDMEREVSGIASRVASRV
                     VTVQASFRNPNDENAPGIVNVGTGLLLDSLGHVITASSVLSGMRVATPVMTVIDNGDR
                     THEALLYDVDSTLRIAVLYVPTMEAGSPIHAKRAEWAPGYFALVVGNSFGVGPAVRMT
                     TVSGQRARDGFWQLSDPATPGFSGAPVFDSRGAFGGIIVGEVAGQAREQRPSPAVMVT
                     ARQLAPVMSRIAALSGNNNRSWLGLSVRPFVQTDGGTALVVSNVIGGSPAAQAGFLPG
                     DVVTRVDTVDVNFVSDLADWVRRSRPGYQATVRIVRQGVEQDIRVTVGSR"
     gene            complement(10118..10672)
                     /locus_tag="PLT86_03625"
     CDS             complement(10118..10672)
                     /locus_tag="PLT86_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT35738.1"
                     /translation="MTCAESQNLFSEYLEGQITGTVQQGLVEHLEACPACAQKLKLMR
                     IMRVRLSKLDRKHLPDTFSFEMRRALLAEMNRENIWLNRLRALLSPRPQTVWSAAVGT
                     AFAAVCFAVLWLTFPPGGQSGWRSDSVTEVDAAQHDQSVRYVLEHLPFDGEPIESTVK
                     DTARVMPVPPSQAREVVQPVSATF"
     gene            complement(10810..11466)
                     /locus_tag="PLT86_03630"
     CDS             complement(10810..11466)
                     /locus_tag="PLT86_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT35739.1"
                     /translation="MSELLFVEQFTRQSEMVTDEDLLAQSLDGSVNAFEVIVNRYKDR
                     LFNFVVRFVGDQETAEDIVQETFLRAYRNRDTFQAVAKFSTWIYTIAGNLAKSELRRR
                     KRWRFLSLGSKDEHGNTFELPDVASNPDRDAETLLAEAKIEDAIARLPDHYREAIILR
                     DIEGLDYDEISTIVGVPLGTVKSRINRGRLRLQEDLGDLACALWKQDEDETTAVRSNP
                     "
     gene            11785..12837
                     /locus_tag="PLT86_03635"
     CDS             11785..12837
                     /locus_tag="PLT86_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT35740.1"
                     /translation="MRAVVYGNDHKLSIQEMPVPSCPRNGLLAKVHACAICGSDVKMF
                     KAPDPRVKPPQIVGHEFVGEIVEVGSDAKGFAVGDRVVMATTLSCMACPACWAGKTNL
                     CPNVMPVSRAFPGAFAPYIAIPWEGIRAGNTVKVPSSLEDDGACLAEPLSCAVNAQQL
                     AGVRTGQTVVVVGGGPLGALNAELAKANGATRVIVSQRSATRQDLLRELDLDAVIDAA
                     SCDPVEEVKRLTNGRGADVVIVTAPDRTAQERALEMACKGGMVNFFAGMSKDDPMVTI
                     NSRLVHYNEISITGGSDSTARHVEIAVGLLAAGRVNWKKIVTHRFPLSRFFDGMQVML
                     DRAGLKVVIKPQEDAQ"
     gene            12881..13672
                     /locus_tag="PLT86_03640"
     CDS             12881..13672
                     /locus_tag="PLT86_03640"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT35741.1"
                     /translation="MRGRLENRVALITGGARGIGKGIAARFLTEGARVAIADLNGEGA
                     TTTAAELGASSKSDCAIGVPVDVTAADSVRRAIEETRSRLGEVEILVNSAGFSKIARI
                     EDTTDEIWDRTLAINLKGMFLMCREVVPAMKQSGWGRIINLSSQSGKKGNSSYTAYCA
                     SKFGVIGVTQALAQELAEDGVTVNAICPGVVFTEMWGPEQVADYARKRNMAPEDVKEY
                     LTSKIPMKRAATVEDVAAVAAFLASDDAAYLTGQSYNVTGGTIMH"
     gene            13684..14805
                     /locus_tag="PLT86_03645"
     CDS             13684..14805
                     /locus_tag="PLT86_03645"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT35742.1"
                     /translation="MKKVTDIHQVDELFAEAREAGVPVLAPNFESFAELVAEFTAAQT
                     IAERFGIKRVPLMPSLTLSYPDRGNVAGRGGAYQDFAVPLNKRYSRDELNRFLRLGYH
                     VSNHDMDCFDGTSYFPNVYAIQTFDHGQPEGDKILLEDPKVLETVGVVMYDVTELLKR
                     RPELREDRKLALKTMAEATARYREAFGKYVRVESACDILPEAPAEGSLPNVEDTLTRP
                     DEMEEFLRISGADFFAPSIGTEHRAKNPEGTEKLYRESLARELHERVAKMPRYAPVDG
                     KIFSLHGSSCLDPSNKNNEGLYRKLHGMVIFRYYTGMANTMGSAVRQWVEDGERRVRT
                     NPKDGLHVNASSGRDWYVIQQLQPELVQVMINIGYAELA"
     gene            14837..16171
                     /locus_tag="PLT86_03650"
     CDS             14837..16171
                     /locus_tag="PLT86_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35743.1"
                     /translation="MNTRPAPLVWHPPSTFDPDAPGLGFPTLDGVSHRVIFDPSPSKA
                     NVDEGGDGKYESLLHGNFCHGPSFVVVGEHIVARWSNHTMDEGGPGARSIGRVGTFIN
                     DGDEIDWGGQETFVELAPPPIPPRRRKFAVDPLNLWPYTGAGLSLVNGSLYALGLIHS
                     VVGYTDEEQYRATRGPVPAEHFRDALDVDAGFFFDKWGYHGLEWVQRWKIVKGRLLPD
                     SPMYRISPYEPRFEVTPGRFKIACPLQEPYCCALPIEETPEALREDVSMLREEALIPK
                     WRFTAGEKWIAADGRHALAHLAEYVRPDGRSVMVRDNLLNPGHYYASLTDENGAYPPA
                     VETNLIGGANIAAGTLPDGRNFIIGNNYDDYYHAPDKSRKDMYITLSRNGVDFDRTYL
                     ILHLDRKGDGGVYKYGGPQYYKPLIHKNNMWVFYSITKMKIGLTKIPLNAFA"
     gene            16217..17209
                     /locus_tag="PLT86_03655"
     CDS             16217..17209
                     /locus_tag="PLT86_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOT35744.1"
                     /translation="MEPVVAQDIPQTLAPDLALSMLRMILTIRIFEEKMLEFLNTGVI
                     KGGSHLYIGQEAVATGVCAALRKDDYITSTHRGHGHGLAKGGDPKALMAEILGKATGV
                     CRGRGGSLHLADLNTGNLGANGIVGGSLGIAAGAALSAKMRDSGQIVACFFGDGAVNT
                     GIFHEAMNMAKVWELPVLFVCENNLYGMSVSVNRATGLTDLADRADGYRMRKAIVDGQ
                     DVFAVWEATRHYGEIARSGGGPTFIECKTYRFGGHSRSDARKYRTREEERKWRERDPV
                     TVCAQRGIEEGWFEAGTVERIRSEVAAIIEEAADFALSSPEPDPSELTDDLYAA"
     gene            17266..18615
                     /locus_tag="PLT86_03660"
     CDS             17266..18615
                     /locus_tag="PLT86_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT35745.1"
                     /translation="MAKPNILLFMMDALRPDRLSPWGHDAVAVGPPHPTPNFARLAET
                     GVFFRNCFSHMPSSHPSRASILTGRDPHTHGVRINSRPLSASETTLTQILRNAGYLTA
                     TTRTFPPGMGRGFEERGLVSKVLSPFDAGPWDIVKELPQMAEDEAGTDVAQDTTALIR
                     WLQRYVADPERRRHPFFLWADCEDTHEPWRPPVPYNTLYDLEPYDGPDVSCPPMYSPH
                     LTERQVRHARALYDGVVALEDAHLGRLLDALERLGLAQDTLLIVMSDHGVHLGENHLW
                     RKPPTLFDAVLRATLIMRLPGVIPQGRHTDGLNLVNDVFATVLDLAELEMPSGAVGQC
                     TSLRPLWEGQQQVRNFVPLEFNLYKGTAGKGIRTERWKYFYYRSVGDLMWDTMSPADV
                     WRGEGWPLTMLFDLENDPAESRNVADEFPQVEREMRDILLNWLIDSENDLPAPVPGD"
     gene            18735..19370
                     /locus_tag="PLT86_03665"
     CDS             18735..19370
                     /locus_tag="PLT86_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT35746.1"
                     /translation="MITDTAVTASLRMEALLSAHTLVLNRHWMAVGFTQVRRAISLVY
                     EDVAKVIAPDTFALYDFDTWADLSRHAPTQDCVRSVRVVFRIPEIIILTTYSGMPRAN
                     VAFSRRNLCIRDRYTCQFCGEKLSSENLTIDHILPRSRGGHATWENCVLACVRCNERK
                     GDRMPEEVGMRLLKQPRRPRWAPSFGIPLRHRKESWEKFISDVYWDIELES"
     gene            19385..19876
                     /locus_tag="PLT86_03670"
     CDS             19385..19876
                     /locus_tag="PLT86_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOT35747.1"
                     /translation="MTDAHEHRLGWDTYFMRIAQLVAERSTCTRAKIGAVIVRERSII
                     ATGYNGSPAGMPHCTEVGCLVYRSTAPDGTVEENCFRTIHAEINAIAQAAKHGNSIDG
                     ASIYITATPCIHCLKVLINVGIKRIFYAKPYKLQTIEEMVRISGIELVQVEVEPLPEE
                     MKH"
     gene            20121..>21806
                     /locus_tag="PLT86_03675"
     CDS             20121..>21806
                     /locus_tag="PLT86_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35748.1"
                     /translation="MPQDRLSDPVTAVTHYPYLLEPAEHPDYERYHVRMPTWETFENR
                     TQLTTLRGFSVQGGKLVAFREELDRYVNAGLGRVIWPFYTTVLAENFWDLVEEVRCRK
                     LWLFDFWGYVPGSPLEGSWCNYQTPPGMVERLERELGDRFLGFDNGEQDGRYIGRFAH
                     QQCPSPQDRVRQYLNFQRHFQRLCDDLGNHMCTLVSLCFGHYFIKEGNHTLIGAETAQ
                     ALPNSQVYYAFIRGAGKQYGVLWFGNASVWNRWGWKVYESSGTDPGGLKWGPDAGTST
                     ALLKRLIYSHWMYNCAAIGFESSYFGADGQLSPIGNIQAAMGRFIEEHGSPGTMHAPV
                     ALMLDHYAGWAMPRHLYSGSGYYGDTSYQVWGGIPYGAGDYLTHEVLSLAYPGYVDAG
                     YYRDERGFLSPTPWGDMMDCLLSDAAPWVLRQYGLIVIAGALSDENAGTETRDKLDAF
                     MASGGHVVATAENARRLWPELGIGDAIRVPAGTTVRFERTGEVFEEPLAFDLHTISAI
                     PDEVVAEAVLEPGSQPAAVRFTRGSGLLTVVLSPFGLNAEPTVSGPVPNEAERE"
BASE COUNT         4519 a         6334 c         6554 g         4299 t
ORIGIN      
        1 tcgctgcctt ccccgccttt ccgtttgcct cggaaaagat gccgggatgg cgaaatatta
       61 aggtagtcac acgccctttc gacggtcaac cgtctcggga ttgtcaattc ccgggcgcaa
      121 acagatcaaa attgcgtctc acgcggtact gggtcccgca gcgcgagggg tttgtccagt
      181 atttccgcca taacgactgc tcgcttgagg tccccgcggc cgattccaaa gcgttttgcg
      241 cggtggacgg gagcggatgg cgcggcgtca gcaacggtac gccgcgagaa actctctcct
      301 tcgtgcgcgt cgatatcggc tgcagacacc ttctgaaccg gccgtggttg agcctcacgt
      361 ttccaatctt ccggcacaaa caccggctcc tgactcggta ccgaagcagt tttcgccgca
      421 cgttcgtcca gttcccgctg catctccttg aaaagggggc gtgccgggac cactggctgt
      481 ggctccgggc gctccttctc ggcgtcctct tcgtccgtgg ccattgcagg gaccggttgc
      541 ggtcgagaag gcggtttcgg tggcgcgggt ttccagagag ggccgaaagg ccccggagcc
      601 acgggaggcg gaccggccgc aggcgatggt ttcgggggct tccccgcctg ttcccgcgcg
      661 cgccgttcag cctcctgttt ttcctttcgt ttgctcagga gcgtggaggc aatgctgatg
      721 atgatgaaga tgatcagcca cgagaaatcc acgctcagct ccttgccggc gggttatccc
      781 cggcttgcgg ggaaacgcct tcgcgcgcaa tggcggatct catctcggtg tccgagagaa
      841 tgtttttcat cctgtagtag tccatgacac cgatgtgtcc cttgctgaac gcttccgcca
      901 tcgccagagg aatctgggct tcagcttcca ccagcttcgc ctgcatttcc tgtgttctgg
      961 ctttcatctc ctgttccagc gcaaccgcca tcacccggcg ctcctcggct ttcgcctgcg
     1021 caatccgctt gtcagcttct gcctgatcgc tctgcaattt cgcgccgatg ttgtccccga
     1081 catccacgtc tgcgatatcg atggaaagaa tctcaaaggc ggttccagaa tccagcccct
     1141 tctccatcac cttcttggaa atcacatccg ggttctcaag cacctgtttg tggctggatg
     1201 ccgaaccaat tgcgctcacg attccttcgc caacacgggc gataatcgtc tcttctcccg
     1261 cgccaccaac gagccgatcg aggttggtgc gcaccgtgac gcgggccttt gctttgagct
     1321 gaatgccatc catcgccacg gcatcgattg tggaccggcc gcgggaggga tcaggacaat
     1381 cgatcacctt tggtgtgatg ctcatcttga ctgcttcgag cacgttcctg cctgcaagat
     1441 cgatcgcggc agcgcgtgca aatggaagct cgatgccggc cttgttcgcc gaaatcagtg
     1501 cggcaaccac aagttggaca tttcctcgcg ccagataatg cgcttccagt tcgtcgatct
     1561 gcagattgag cccggctttc gtcgaagtga tcagcgaaag gacaattcgc gcggggggca
     1621 cattccgcag acgcatgccg atgagcgtgg aaatcttgac cggaacattc gcaaactgcg
     1681 cttcgatcca cagccgcacc ggaatgaaat acgtgaacag gatgaggaaa acgattactg
     1741 cgaacacgcc gaccgccaca aggagcgtct gatcgtacat tgcaatccct ttctggtcta
     1801 cgcggcgttg actccgcgcc gctggcactt ttcgaaaccg ttaacgggaa aactcacagg
     1861 gctttcacct ttatgtggtt cccttctatc tgaacaacct ccactgcttg tcccgcctcg
     1921 gcgatggggc ccaggactgt tacgtcgtac cttctgtccc ctatctgcac gatccccgcc
     1981 ggccgcaact tcgttacggc cactccccgc gcaccgacct gcggcagtaa ttcgcgcgct
     2041 ttggtttcat cccacacctt gccgggagaa acggacggat ttcggagttg cttcatgcca
     2101 cccgtccggg cggtgcggga aatcatcagt accgatccca acaatgtcac gcaaaaaacc
     2161 accaccgcct gccatgttcc gagatggaga ttcgcgtacc ataccgcccc gagaattgcg
     2221 gcaatcgcgg cgattcccgt gattccgaac cccggaatca cgaacagctc aagcatgagc
     2281 aacacaaagc cgaggagcgc aagagctatc acaaatgccc acgtaacaac catttccgcg
     2341 ccttccgcct tccgcgaggc tgacaattac acccttcgaa caacaagacc ccggagacca
     2401 tccacttcca ctactttgac ggacgcaccg ttttcaatat gctcgccgtc agtaactgca
     2461 tcgtagtact tgccgccgat cgcaacgcgc cctaccggac gaagcggcga atcggcaacg
     2521 cccacggcac cgagaaggtt cggttgtggc cccgctgccg aagcggtctg accagttccc
     2581 gggttctggc tcttttccag catcaacttt cggaacattg gcaattgtga aaaggacttg
     2641 atcaagacca gcgcaccgat gaacgtcagc acgacggcca tgccgataga agtgaacgca
     2701 tgcgtcagat cgccggggct cacgagatcg attctgccca ccagactgag gaacagaccg
     2761 gtcagcacga gcgcgccgcc gatgattccg agtattccac ccggcacgaa caactccgcc
     2821 acaatcaacg caactcccgc cgcaaaaagc agaagttcct caatctgtgc aagccttact
     2881 atccagtgtg ccccgaaaaa gagcaccagg cacgttatgc cggcaatccc gggaagaccg
     2941 aaccccgggc tcgtgagttc gtaaatcact ccaagggcgc ccaccgtcat gagaaggcca
     3001 ctcaccacag gatgcgtgag gaaccggacg atgtgttccg cccagttcgt ttcgaggcga
     3061 accattttgg cgccctcgag accgttgtag cggagggcct ccgcaagcga ggcagcctgc
     3121 atctgggcaa caccgaacgc gatcgcctgg tcggtgcgca acgtgagcgg tcttggcgcg
     3181 ttttccaggc cagggacaac gatcgaggaa tccaccatgg cttccgcgag acgcgggtcg
     3241 cggcccttcg cgcgcgctgt ttcgcccatg atgctacgga aataggaaac aaccttgttg
     3301 gtcgcaacgg cgccactctg atctactggc gtggccgcgc caatagcact tcccggcgac
     3361 attacaatga catctgtcgc gtaggaaatg agagctcccg cggatgccgc gttgttgtga
     3421 atgaacgtga ttgttcggac gtcggtattc aggagtgccg ttctgatttc gtcggcggcg
     3481 tcaacacgtc ccccgtaagt gttcatctct atcacaaacg ccgcggcgcg cattgctttc
     3541 gcttcatcaa gcgcgcgccc gatgaatgcg gcaaggcccc cgtcaatcat cccctcgacc
     3601 tgaacaaccg cgactgtctt ccccctgccc gccatgcgcc ctgcagggaa ctcggattcg
     3661 agacgctgcc agaggctgtc gcgaagaccg gctttcgggg aaacggaatc agccgccggg
     3721 gttgctttcc gcaacgaatc ggcagcttcg ccgctcgcac ttgcgccaac aagcaaaccg
     3781 cctgcaatca ggataaaata agtggtgcgc cacgcggaac gccgcatatt ctgcaccccc
     3841 aacgggagtc tcgttacaac atgtaacaat acatcttacg acaaacaccg gaataggtca
     3901 acaatatgcg cgcccgttgg ctttttggcg cctgcgtgtg tttcgttaaa aggggaaaca
     3961 cagaaggaaa ggatgcgttg tccgtggaca gattctacat cacaaccccg atttattacg
     4021 tgaacggccc gccgcacctc ggccatgcgt acaccaccgt tgtagccgat gcactcactc
     4081 gttttgctcg tcttgagggt aaggacgcgt tcttccttac cggcacagat gagcacggcg
     4141 agaagatcgc gcagagcgcg caatccaaag gaatcgcgcc gcaaaaatgg gtagacgacg
     4201 tcagcgccca gttccgcaaa ctctggcctg aactccacat agaacccgac gatttcatcc
     4261 gcacaacgga ggagcgccac atacgcgtgg tgcaagcgat cctccagaaa gtgtttgaat
     4321 ccggggacat ctattatggc gagtacgggg gcaactattg cgtcggttgc gagcggtttc
     4381 tcgatgatga cgaactggtc gacggcaaat gccctgatca cggcgttgcg ccgcgcttcg
     4441 tgaacgaagc gaattacttc tttcgcatga gcgggtatca ggactggttg atctcccaca
     4501 ttcaagaaaa cccggagttc atccgcccgg agcgctatcg caacgagatt ctcgcgttcc
     4561 tcaggaaacc gctgaaagat ctttgcataa gccggccgaa aaaccgcctt tcctggggaa
     4621 ttgagctgcc ttttgacagc aagttcgtca cgtacgtgtg gttcgacgcg ctggtgaatt
     4681 acgtatcagc gctggggtgg ccggacgggg agcggttcgc gaaatactgg ccggtagccg
     4741 aacacatcac ggccaaggat atcatcaaac cacacgggat ctactggccg tgcatgctca
     4801 aagcggcagg cattcctgtg taccgccacc tcagcgtgca cggcttctgg cggggcgctg
     4861 acgggcggaa aatgtccaag tctctgggca attctgttga tcctgcggaa atggcgcaac
     4921 ggtacggcgc ggacgttttt cggtacgcgt tgttgcggga aatgccctac gggcaagatg
     4981 cgaatatcgg tgaggaaatc atcgcgacgc ggcagaacag tgatctggca aacgatttgg
     5041 gcaacctgat ctcgcggacg ctgaagctgg tgttccgttc tttcggcggc gtcgtgccgc
     5101 gtgtgtcgga aacgcgcgtt gaagataccg cgcttcgtga ccaatggatc ggtgcgctga
     5161 cggtcgtgcg cgaggcgtgg catgaacttc gtatgtcgca ggggatagaa accgtaatcg
     5221 attgcatacg agcaacaaac cgatattttg accagatgaa gccgtgggag cttgcaaagc
     5281 gcggtgatac ggaacgtttg gcgacagtat tggtatccgc cctcgactcc atccggatcg
     5341 cgtccgtgct gctgactccc gtgatgccag aacgcatggc ggaactccgc aggcagcttg
     5401 gccttacctc atcgccttct ctggaagagg cagaacgctg ggacgggttc gccgaaggcc
     5461 accggcttgc ggaaggacaa tccctttttc cccgagttga cctcgaagag gttcgcacgc
     5521 gggagctcca tcgccgtgag gccgcgactc aatctggaac cggcgacaaa cccgagccgg
     5581 cgccggtgga aagcgctgca ccagtaacca tcgaggaatt ccgccgggtt gaccttcgtg
     5641 tgggcaccat catttccgca gaacccgtcc cgggggcccg gaagcttctc cgactccagg
     5701 tggacatagg tgaagctgct cccagacaac tcgtcgcagg gctggccgag caattcgaac
     5761 cggctcacct cctcgggagg caggtcgtcg tcgttgcgaa tcttgaaccg gcaattatcc
     5821 ggggtataga atctcaggga atgattctcg ccgcggacga tcagggccgt ctggcacttc
     5881 ttggccccgc tgccgccatg ccaaacggag caaggatccg ttaacacccg agggactcgt
     5941 tcagaacaaa gtgggaaggt agtgctgcgt tgctggatct gtatcggtgg cacctccgtg
     6001 ctcggcggat gaataacccg ggtgcgattg cggtggggtt ctattttctc tttgctccac
     6061 cgacactcct gcctgccggt gaccaggtcc acactctcac cggccatggc agccccgtga
     6121 tggcactcgc atgcacgcgg gacagcagat acatcctttc ggccggcaga ggacaggtta
     6181 ttcagtggaa tgtgcgcaac gggtcgcggg tgttcaccct tattcacccg gggtgggctc
     6241 tcagcattgc cgtcgcgccg gatggctcac agttcgcaac tgggtccgat gacggcctca
     6301 ttcgtgtctg gcggctggac aatggtggtt tgttgcggac tctggaggga cacaggggcc
     6361 ttgtggtggc gctggcgtac agcgatgacg ggacaaccct tgcgtccgca ggttttgatt
     6421 cggtggttgc catctgggac gttgccggca ccgaagccgt tcggctcctc cggggtcacg
     6481 caggggaggt gaatggcgtt cttttcagcg caacgggcga caccgtcttc acgtgttccg
     6541 cagacggcac cactcgtctg tggaacacgc acgacgggtc cctgcttcag gtgtttcgcg
     6601 ggcattcgga ttcagtccga ggggtggctc actcgcggcg cgagaacatc ctggcaacgt
     6661 gcagcaacga ctccacggtg cgcctttggg acgtcaagag cggagaatgt gtgcgcgtcc
     6721 tgcgtgggca ttccgatgcc gtatccgcag ttgcgttcag tccggacggg cggtttctcg
     6781 tttcgacggg ttcggaccgg actgtgcgcg tctggcgggt ggacaccggc gagacggagc
     6841 gagtgttgag aggacatacc gattggatcc gcccgctgtt gattacgcgt gacggcaacc
     6901 acatcgtaac gggcgcgagt gacgccaccg tgatggtatg gcggcgcccc tggtaacggg
     6961 gagacgtacg gtgttctgca ccgcccagtg ctcacattag tcaacgcaag cacagactga
     7021 aggaaactta ccgtggcaca cggtacccgg ggttatcccg ggaaccagat ttcttggagg
     7081 gaagaaccgt gacaaagctt cgtctggcat gtgtcggcgc gggccccgac aaacactccc
     7141 gcagcagaaa ctatctcgcc gtcgtgaagg ctctacacga tctgtacgag ctgtgcgcgg
     7201 ttgtggatcc aaacgaatcg aacgcccggg aagcggcagc tgaatacggt attcccggcg
     7261 tgtacacaga tatggaaaag gcaattaagg agacaagacc cgatgtggtg ctccgccttg
     7321 cgccgacaga ttctgccatg gcgatttgcg taacggctgc gcagatgggt tgcaacgtca
     7381 tcagcgaaat tccgattgat atcacgctcc ctcgagcgga tgctaccatc aaggcctgcc
     7441 gcgagaataa cgtcaggatc gagatcgcgg agaacgtgtg gctctggccg gaagagcagc
     7501 tgaaacagga aatcgtgcgg aggggaatta tcggaagacc ggttcacgca cgcctgaagt
     7561 atccctgtgg aagctaccac ggcttcaatg gcgttcgaat gatcctggct gaggaagcag
     7621 ttcgcgtgct cgggttcggt ggaacggtgg aggtgatgcc gcttcctgcg tacgcaggcg
     7681 gcatgatgac gcacgtccag tgggacggcg cctgtgtgga gttttccagc gggctcaagc
     7741 ttttgttcga aatgccgccc aaaaagcctg tctgggaacg caactgggat atcatcggca
     7801 cgcatggcta cttgaccggt gattcactgg tgttgtatcg cgaaggacat caccacggtt
     7861 cgggcgaaga agagcgtttc cccatcgaat cggtatatcg cgagattcag gggcaacgtg
     7921 tgcttcaggc ggtccgggtg aatacggacc cggtcgttga atgggtcaat ccctacattc
     7981 gctacggcat ctccggaacg gacgatatcg cgaaagcggc gatcctcgag agnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaagaaat ttcgtgcgcg cacgtgtgct
     8161 cccttgacct atttgttcag tgccgctacc attagcacag gaacttgagc atttgaacac
     8221 gttctgtcct gaatagattg cctgcgaaag gaaggtatgc gggatgttgg atgcactcgg
     8281 tatggtggaa actaagggct tggtgggcgc aatcgaggcg gcggatgcga tggtcaaagc
     8341 agccaatgtg acgctcatcg ggaaagagac aattggcgga gggtacgtta cggtactcgt
     8401 tcgcggcgac gtcggtgcag tgaaagccgc gacggaagct ggttccgccg cagcgcaacg
     8461 agtaggcgag ctggtgagcg tgcacgtgat tccgcgtccc catgcggaag tcgagaagat
     8521 actcccgaaa gcatagacgg gatccgctcc catgcagact gactcagcca acctggcact
     8581 cgggctcctc gagacccggg gctataccgg cctcatcgcg gctacggatg ccgcgacaaa
     8641 gaccgcgggc gtcgaagttc attcgatcgc ccgcgcgaca ggtggactcg ttcttgttgc
     8701 gcttgtcggc gatgtcgcgt ctgtgcaggt tgccgtggcc gccggtctgg agagagctca
     8761 agcagtggga agccctatct cagcccatgt gattgcgcgg cccgacgacc aggtctggaa
     8821 gtcaatcggc ttcccgtcgc cagcggacaa ggggccttct ccagttgctg aatcggcgca
     8881 gccgaatacc aaggaagacg atttggacgc gcttcccgtg cgcgcattgc ggcacatggc
     8941 tcgctcgtta ccggtcgggc tgaccggccg ccagatatcg agcgcatcgc gcgatcagat
     9001 tctttccgcg atccgtgcgg cgcgttctca caagtaacca gaatcacgaa ttgaaggggc
     9061 ctcccggcga ttgaattcct cgtcgggagg cccttttcgt tgcaccggct gtcaggccgt
     9121 gagacactca cctcgagcca actgtaaccc gaatgtcctg ttccaccccc tgccgtacga
     9181 ttcgaacggt tgcctgatat ccgggacgtg accgtctcac ccagtccgcg agatcgctga
     9241 cgaaattcac gtcgacggta tcaacccggg taaccacgtc gccgggcaaa aagcccgcct
     9301 gtgcagcggg gctcccgcca atcacgttgc tcaccaccaa cgcggtgcct ccatccgtct
     9361 gcacgaatgg acggacagac aggcccaacc atgagcggtt attgttcccg gaaagcgccg
     9421 ctattctgct cattacgggg gcaagctgac gcgcggtaac catgactgcc ggcgacggcc
     9481 gttgttcgcg tgcctgaccg gccacttctc cgactatgat ccccccgaac gcaccgcggc
     9541 tgtcgaacac cggggcgccg ctgaaccccg gcgtcgccgg atcactcagt tgccagaatc
     9601 catcccgtgc ccgttgcccg gatactgtcg tcatccgaac ggcgggaccg accccgaacg
     9661 agttacccac gaccagcgca aaataacccg gagcccattc cgcccttttg gcgtgtatcg
     9721 gcgagcccgc ctccatcgtc ggcacgtaaa gaaccgcaat tctcagcgtt gagtccacat
     9781 catacaacag cgcctcatgc gttcggtcgc cattgtcgat tacagtcatg acgggggttg
     9841 cgacacgcat tccggaaagc acacttgagg cggtgattac atgccccagg gaatcaagaa
     9901 ggaggccagt gcccacgttg acgattccgg gagcattctc gtcgttcgga ttccggaagg
     9961 atgcctgaac agtcaccacg cgggaggcga ctctggaggc gatgccggac acctcgcgct
    10021 ccatgtcctg aagtacagtg gaaccggcgt ggtcggcaga gacagctacc gtcaacgcgg
    10081 ccaccagttt caatacccgg tttggcattg caggaattca gaacgttgcg cttaccggct
    10141 ggaccacctc gcgggcctgc gaggggggaa ccggcatgac gcgcgcagtg tctttcaccg
    10201 tggattcgat gggctcaccg tcaaagggca ggtgttccag cacgtaacga acggactggt
    10261 cgtgttgagc cgcatccacc tcggtcacag agtccgaccg ccagccagat tgcccgcccg
    10321 gggggaacgt cagccagagt acggcaaagc acactgccgc aaatgcggtg cctactgctg
    10381 cagaccacac ggtctggggc ctgggggaga gcagtgcacg gagacggttc agccatatgt
    10441 tctcgcgatt catctccgcg agaagggccc gtctcatttc gaaactgaac gtatccggca
    10501 ggtgttttcg gtccagcttc gacagccgca cgcgcatgat gcgcatgagc ttgagctttt
    10561 gagcacacgc agggcacgct tcgaggtgct caacgagtcc ttgctgcacg gtgccggtga
    10621 tctgcccttc caggtactcg gagaacagat tctgagactc agcacacgtc atcggattgc
    10681 ctccaatcgc attgaatcag ataagacctc ccgttcacgt ttgtagttca gacaacgccg
    10741 caggattgcc ggttccgtcc gtaaccggtt ttgaccgcgg tacgaggtcg aaacgcagcc
    10801 actcgcagtt tacggattag atcgcactgc cgttgtttcg tcctcatcct gtttccagag
    10861 agcacatgca agatcgccga ggtcctcctg cagacgcaag cgcccccggt tgatccttga
    10921 tttcacggtg cccagtggaa cacccacaat cgtggagatt tcgtcgtaat ccagcccttc
    10981 aatatcgcgc aggatgatcg cctcgcggta gtgatcaggc aggcgagcga tggcatcctc
    11041 gattttggct tccgccaaga gagtttccgc atccctgtcg ggattactgg cgacgtcagg
    11101 aagctcaaac gtattgccgt gctcgtcttt tgagccgagg gacaggaatc gccacctttt
    11161 gcggcgccga agctcgcttt ttgccaggtt gccggcaatg gtgtagatcc acgtcgaaaa
    11221 cttcgccacc gcctggaacg tatcgcggtt tcgatatgcg cggaggaagg tttcttggac
    11281 gatgtcttcc gccgtctcct ggtcgccaac gaaacgaacg acgaagttga aaaggcggtc
    11341 tttgtaccga ttgacgatta cttcaaaggc gtttacgctg ccgtcgaggg attgtgcgag
    11401 caggtcttcg tcagtcacca tttcgctttg ccttgtaaac tgctccacga aaagtaactc
    11461 gctcatgcac ggacccctcg ctctggttcg gagaacgctg ccaacgaata gtaggacgtg
    11521 caaagggcat ttcggttccc aagttctcgc ggacctggtg gagaagcaac cttcggctcg
    11581 gggtcgcccc ccatttaatt atacgacgaa ggcacgtgtt cggcggacgc gaaacgcttc
    11641 gtcctttttc tcgcggacgc tgacaccgag ggaatccaaa aacggtcctt ccgctgttgg
    11701 tcggcctctc aaacgggtgt atttttccca atggccggtt cggagaacat tgccagaaca
    11761 acctgaagac gtaaggagtc ccccatgcgc gccgttgtgt acggaaacga tcacaagctc
    11821 tccattcagg agatgccggt cccgtcatgc ccgcggaacg gtttgcttgc gaaggttcat
    11881 gcgtgcgcaa tctgcggttc cgacgtaaag atgttcaaag cgcctgaccc cagagtgaaa
    11941 ccgccacaga ttgtagggca cgaattcgtg ggagagatcg tggaagtggg ctccgacgcg
    12001 aaaggatttg cggtcggcga ccgggtggtg atggcgacaa cgctctcgtg tatggcgtgc
    12061 cccgcatgct gggccggaaa aacgaatctc tgcccgaacg tgatgcctgt cagccgtgca
    12121 ttcccgggcg cgttcgcgcc ctacatcgca atcccctggg aaggcattcg cgccggaaac
    12181 accgtgaagg taccgtcgtc gctcgaggat gacggtgcat gccttgccga gcccctctca
    12241 tgcgccgtca acgcccaaca acttgccggc gtacgcaccg ggcagaccgt ggtcgttgtg
    12301 gggggcggcc ccctcggtgc acttaacgcc gaacttgcga aggcaaacgg cgccacccgg
    12361 gtgatcgtga gccaacgctc agcgacgcgg caggatctcc tgcgggaact cgacctggac
    12421 gcggtgatag acgccgccag ttgcgacccg gttgaagagg tgaagcgcct gacaaacggg
    12481 cggggagcag acgtggttat tgtgaccgcc cctgatcgga cggcacagga acgggctctg
    12541 gaaatggcct gcaaaggggg catggtcaac tttttcgccg gcatgagcaa agatgacccg
    12601 atggtgacaa tcaacagccg gctggtacat tacaacgaga tttcgattac cggaggctct
    12661 gattccacgg cgcgacacgt ggaaattgcc gtgggcctgc ttgctgccgg acgcgtgaac
    12721 tggaaaaaga tcgtcacgca ccggttcccg ttgagccgtt tctttgacgg catgcaggtg
    12781 atgctggatc gcgccgggct gaaagtggtg atcaaacctc aggaggacgc ccagtagaaa
    12841 cgtcgcgggg gctgttcaaa ccgagagagg caaacggatt atgcgaggaa ggcttgaaaa
    12901 ccgtgtagct ctgattacgg gaggtgcacg agggataggg aaaggtatcg cggcgcgatt
    12961 ccttacggaa ggagcccgcg ttgccatcgc cgacttgaac ggggaaggcg caacgaccac
    13021 tgccgcggag ctcggcgcat cttcgaagag tgattgcgca atcggcgtgc ctgtggacgt
    13081 gactgccgcc gattctgttc gtcgcgcgat agaagagacg cgttcccggc tcggagaggt
    13141 ggaaatcctt gtcaacagcg cgggattttc caaaatcgcg cgtatcgagg atactacgga
    13201 cgagatctgg gatcgcacgc tcgccataaa tctgaagggc atgttcctca tgtgccgtga
    13261 ggtcgtcccg gcaatgaaac aaagcggttg gggccgtatc atcaatcttt catcccaatc
    13321 gggtaagaag gggaatagtt catatacggc atactgcgcg tcgaagttcg gagtgatcgg
    13381 ggtgacgcag gcgctggcgc aggagctggc ggaagacggg gttacggtga atgcgatctg
    13441 tcccggcgtg gtgtttaccg agatgtgggg ccctgagcaa gtcgccgatt atgccaggaa
    13501 acggaacatg gcgcccgagg acgtgaaaga gtatctcacg agcaaaatac cgatgaagcg
    13561 tgcggcgact gtggaggacg tggcggcagt cgctgcgttt ctggcctccg atgacgccgc
    13621 ctacctcacc gggcagagct acaacgtcac cggcggcacc attatgcact gagggagcac
    13681 acgatgaaga aagtcacgga catccaccag gttgatgagt tgttcgcgga ggcccgcgaa
    13741 gcgggtgtgc ctgtcctcgc accgaacttc gaaagcttcg ccgaactggt ggccgagttc
    13801 accgctgcgc agactatcgc ggagcgcttc gggatcaaac gtgttcccct gatgccttcg
    13861 ttgactcttt cctatcccga ccgggggaac gtggccggaa gggggggagc gtaccaggac
    13921 tttgcggtgc cgttgaacaa gcgctactcc cgcgatgaac tgaataggtt ccttcgcctg
    13981 gggtatcatg tttccaacca tgacatggat tgcttcgatg gcacgtcata tttccctaac
    14041 gtgtacgcga ttcagacctt cgaccacggg caaccggaag gggacaagat ccttctggaa
    14101 gacccgaagg tgctcgaaac ggtcggcgta gtcatgtacg acgtgaccga actactgaag
    14161 cgccggcctg agctgcgaga agatcgaaag ctcgcgctga aaacaatggc agaggcgacg
    14221 gcacgttacc gggaggcatt cggtaaatac gtccgagttg aatcggcctg cgatatcctc
    14281 cccgaggcac ccgcagaggg atccctgcct aatgtggagg acacccttac ccgccctgac
    14341 gagatggagg agtttctgag gatttctggc gcggactttt tcgcaccgtc gatcggaacg
    14401 gagcataggg ccaaaaaccc ggaaggaacc gagaagctgt accgggaatc ccttgccagg
    14461 gagttacacg agcgcgtggc aaaaatgccc cggtacgcgc cggttgacgg caagatattc
    14521 agcctgcacg gttccagttg tcttgacccg tccaacaaga acaacgaagg cctgtacagg
    14581 aaacttcacg gtatggtgat cttccggtac tataccggaa tggccaacac catgggtagc
    14641 gcggtcagac aatgggttga ggacggcgag cgcagggtaa ggacgaaccc gaaggatggt
    14701 cttcacgtga atgcatcaag cgggcgggac tggtacgtga ttcagcaact gcagcctgaa
    14761 ctggtccagg tgatgatcaa catcggctac gcagagctcg cttgatacgc attattgcat
    14821 gagagggaca acaacagtga atacacgccc agctccgctt gtctggcatc ctccttccac
    14881 attcgacccc gacgcaccgg gccttggctt cccaacgctc gatggagtat cccatcgcgt
    14941 gattttcgac ccttcgccat ccaaagccaa cgtggacgaa ggtggggatg gcaagtatga
    15001 aagcctcctg cacggcaatt tctgtcatgg gcccagtttc gtggtggtgg gtgagcacat
    15061 agtcgcgcgg tggtcgaacc acacgatgga tgaaggcggg ccgggcgcgc gttccatcgg
    15121 gcgcgtggga acgtttatca atgacggcga cgagatcgac tggggcggcc aggagacgtt
    15181 cgtggaactg gcgcctccac cgattccccc gcgccgccgg aagttcgccg ttgacccgct
    15241 caatctatgg ccgtacaccg gtgccggttt gtcgctggtc aacggctcac tttacgcctt
    15301 gggattaatc cactcggtgg ttggttacac ggatgaagag cagtacaggg caacgcgcgg
    15361 cccggtcccc gcggaacatt tcagggacgc gctggacgtc gacgcgggtt ttttctttga
    15421 caagtggggc taccacggac ttgagtgggt gcagcgctgg aaaatagtga aaggacggct
    15481 tctaccagac tccccgatgt accgcatcag cccctacgaa ccgcgttttg aggttactcc
    15541 agggcggttc aagatagcct gtccgctgca ggaaccgtat tgctgcgctt tgccgattga
    15601 ggagacgccg gaggcgttgc gcgaggatgt gagcatgtta agagaggaag cgctcatacc
    15661 aaagtggcgc ttcaccgcgg gcgagaaatg gattgcggcc gatggcaggc atgcactggc
    15721 acatttggcg gagtacgttc gtcccgatgg cagaagcgtc atggtccgcg ataatctgct
    15781 gaatccggga cattactacg cctcccttac cgatgaaaat ggcgcgtacc cccctgcggt
    15841 ggaaaccaac cttatcggcg gcgcgaacat tgcggcggga acgttacccg atggaaggaa
    15901 cttcatcatc gggaacaatt acgatgatta ttaccatgcg ccggacaaaa gcaggaaaga
    15961 catgtatatc actctttccc ggaacggcgt agacttcgac aggacatacc ttatccttca
    16021 tctggacagg aaaggcgatg gaggagttta caaatacggc ggcccgcagt actacaagcc
    16081 tttgattcac aagaataaca tgtgggtgtt ctacagcatc acgaaaatga agatcggtct
    16141 tacgaagatt ccgttgaatg cgttcgcgta atgacggagg agtcggttgg aacaaacccg
    16201 gcgaccaggg aagcacatgg agcccgttgt ggcacaggac atacctcaaa ctctcgcacc
    16261 tgatctcgcg ctgtcgatgc tccgcatgat tctcacgata cgcatattcg aggagaagat
    16321 gctggagttc ctgaacaccg gagtgatcaa aggagggtcc cacctgtaca tcggccagga
    16381 ggcggttgcg acaggcgtgt gcgcggcatt gcgcaaggac gactacatca caagcactca
    16441 tcgcggtcac ggccacggac ttgcgaaggg aggcgacccg aaagccctca tggcggaaat
    16501 cctcggaaaa gcgaccggag tatgtcgggg tcgcggcggg agccttcatc tcgcggacct
    16561 caacactggc aacctggggg caaatggcat cgtgggcggg tcactcggta ttgcggcagg
    16621 agctgctctt tcggcgaaaa tgagggattc cgggcaaatc gtggcctgct tcttcggcga
    16681 cggggctgtg aatacgggaa tcttccacga agcgatgaac atggcgaagg tgtgggagct
    16741 tccggtgctg tttgtgtgcg aaaacaacct ctacggaatg tccgtgagcg tcaacagggc
    16801 gaccggattg acggacctcg cagatcgggc ggacgggtac cgcatgcgga aggcgattgt
    16861 ggacgggcag gacgtgttcg ccgtgtggga agctacccgt cattacgggg agatcgcgcg
    16921 atccggaggt ggccctacct tcatcgagtg caaaacctac cgctttggcg ggcattcccg
    16981 ttctgacgca aggaagtacc ggacgcggga agaagagcgc aaatggcgcg aacgggaccc
    17041 ggtcactgtg tgcgcgcagc gcggcattga agaaggatgg tttgaggcgg gtaccgtcga
    17101 acggattcgg tctgaggtgg cggcaatcat tgaagaagcc gccgatttcg ccctgtccag
    17161 cccggaaccg gaccccagcg aactcaccga cgatctctac gccgcgtagc gatttgttcc
    17221 ccacaacgac agcgtttttt gcaccttaca gggagatccg gattcatggc gaagcccaat
    17281 attctcctgt tcatgatgga cgcattgcgc cctgacaggc tcagcccgtg ggggcacgat
    17341 gccgtcgcgg tcggtccacc ccacccaacc ccgaatttcg ctcgacttgc cgagactggc
    17401 gttttctttc gcaattgctt ttcgcacatg ccatcgagcc atccgtccag ggcaagtata
    17461 ctgacagggc gtgaccccca tacccatggc gtaagaatca attcccgacc attgtccgcc
    17521 tcagaaacca cgctgacgca gatcctgcgt aacgccgggt atttgacggc caccacccgg
    17581 acgttccccc ccggcatggg acggggattt gaggagcggg gcctcgtctc caaggtgctt
    17641 tcgccgttcg acgcggggcc atgggacatc gtgaaagagc tcccacagat ggcggaagac
    17701 gaagcaggca cggatgtggc gcaggacacc accgccctca tcaggtggct gcaacgttac
    17761 gtcgccgatc cggaacggcg gagacacccg ttctttttgt gggcggactg tgaggacacc
    17821 cacgaaccct ggcgcccgcc ggtgccgtac aacacgttgt acgaccttga accttacgac
    17881 ggaccggacg tgagttgccc accgatgtat tcgccgcacc ttacggaacg gcaggtcaga
    17941 catgcgcggg cactttacga tggcgtagtc gcgctggagg acgcccacct cgggcgcctt
    18001 ctcgatgcgc tggaaaggct gggcctcgcg caggacacgc tgttgatcgt catgagcgat
    18061 cacggtgttc atctgggcga aaaccatctg tggcgcaaac cgccaacgct gttcgacgca
    18121 gttctccggg caacactcat catgcgcctg ccgggcgtga ttccgcaggg ccggcacacc
    18181 gatggtctca acctcgtcaa cgatgtattc gcgacggtac tcgacctcgc tgaattggag
    18241 atgccctcgg gggcggtggg tcagtgcacg agccttcggc ctctgtggga agggcagcaa
    18301 caggtaagaa actttgtacc gctcgagttc aatctgtaca aaggaaccgc tggcaagggc
    18361 atccgcaccg aacggtggaa gtatttttat taccgatctg tcggcgacct gatgtgggat
    18421 acgatgtccc cggcggatgt atggcgcgga gaagggtggc cgttaacgat gttgttcgac
    18481 ctcgaaaacg accccgcaga atcgcggaat gtggccgacg aattcccgca ggtcgagcgg
    18541 gagatgcggg acattctgct taactggctc atagactcgg aaaacgacct ccccgcgccg
    18601 gtgccgggag attagaacct ccctgcattt gacacgaggc gagccaccgg gctggcgcac
    18661 gcggggtgtg tccgtttaca tttcaagttc tgaagcatcg ggttcgcagt atttcctcca
    18721 tgcgctggaa ggaggtgatc accgacaccg cggtaactgc ttccctgcga atggaagcgt
    18781 tgctctccgc gcacacgctg gtgttgaacc gtcactggat ggcggtaggt ttcacgcagg
    18841 tgcgccgcgc aatcagcctc gtgtacgagg atgtggccaa ggtcatcgcg cccgacacct
    18901 ttgcgctgta tgactttgac acctgggcgg acctttcccg gcacgcgccc acgcaggact
    18961 gtgtccggtc cgtgcgcgtg gtgttccgaa tccccgaaat catcatcctg accacctaca
    19021 gcggcatgcc gcgagccaac gttgccttct cccgccggaa tctttgcata cgcgaccgtt
    19081 acacctgtca gttctgcggc gagaagctct ccagcgaaaa cctcacgatc gaccatattc
    19141 tgccgcgttc gcggggtggt cacgccacgt gggagaactg cgtgctggcg tgcgtccgat
    19201 gcaacgaacg caaaggagac cggatgccgg aagaagtagg gatgcgactt ctgaaacagc
    19261 cgcgacggcc caggtgggca ccttcattcg ggattccgct ccgccaccga aaagagagct
    19321 gggagaaatt catctccgac gtctactggg atatcgaact ggagtcatag gatagggttc
    19381 gcggatgact gacgcgcacg aacacaggct cgggtgggac acgtacttca tgcggatcgc
    19441 gcaactggtg gccgagcgtt ccacctgcac gcgggcgaaa atcggtgcgg taatcgtgcg
    19501 ggaacggagc attattgcga ctgggtacaa tggttctccg gctggaatgc cgcactgcac
    19561 ggaggtcggt tgcctcgtgt accgctcgac cgcgccggac gggacggtgg aggagaactg
    19621 tttccgcaca atccacgcgg aaatcaacgc catcgcacag gccgcgaagc acgggaacag
    19681 cattgacggc gcgtccatct acatcaccgc gacaccgtgc attcactgcc tcaaggtgct
    19741 gataaacgtc ggcatcaaac gcatctttta cgcgaagccc tacaagctgc agacgattga
    19801 agagatggtg cgcatcagcg ggattgaact cgttcaggtg gaggttgaac cactgcctga
    19861 ggagatgaag cactgactgc gtcgcaagcg cgagcgttgc tctgttctgg attccgctcg
    19921 tattgcgcat caccaactga acgtagtcgt cctaatgatg acggcggttg gtgaatgttc
    19981 gccggggcgc acggcgttct cggcgagagt gccctggctc cttggtggcg cggggtactt
    20041 gccccgctga taccccctcg cgatcatacg ttttcaggtc accgtattcc ctccccctcc
    20101 tgacgcagga accagcaacc atgccacaag accgactctc cgatccggtg acggcagtca
    20161 cccactaccc gtacctcctc gagccggccg agcacccgga ttacgagaga tatcacgtgc
    20221 ggatgcccac ctgggagacg ttcgaaaacc gcacccagct caccacactc cgcggcttca
    20281 gcgtccaggg cgggaaactc gttgcgttcc gggaggagtt ggaccggtat gtgaacgcgg
    20341 ggctggggcg ggtgatatgg ccgttctata cgacggtcct tgcggagaat ttctgggacc
    20401 ttgtggagga ggtgcgctgt cggaagctgt ggctgttcga tttctggggg tacgttccgg
    20461 gttcgccgct tgagggatcc tggtgcaact accagacacc accgggtatg gtggaaaggc
    20521 tggagcggga actgggcgac cgcttcctcg gattcgacaa cggcgaacag gatgggcgct
    20581 acatcgggcg ttttgcgcat cagcagtgcc cgtcgccgca ggatcgagtg cgccagtacc
    20641 tgaacttcca gcggcatttt cagcgccttt gcgacgatct cggaaaccat atgtgcacgc
    20701 tggtttccct ctgcttcggg cattacttca tcaaggaggg caaccacacc ctcattggcg
    20761 ccgaaaccgc gcaggcactg cccaactcgc aggtgtacta cgcgttcatt cgcggcgccg
    20821 gaaagcaata cggcgttctc tggttcggca acgcgtcggt gtggaaccga tggggatgga
    20881 aggtgtatga gagctccggc accgaccccg gcgggctgaa atggggccca gatgccggaa
    20941 ccagcacggc gctcctgaag cggcttatct actcccactg gatgtacaac tgcgccgcaa
    21001 taggattcga gtcgtcgtac ttcggcgcgg acgggcagct ttcgccgatc gggaacattc
    21061 aggccgccat gggcaggttc atcgaagaac acggctcgcc cggaaccatg cacgccccgg
    21121 tggctctcat gctggatcac tacgccggtt gggcgatgcc gcggcacctc tactccggca
    21181 gcgggtatta cggcgacacg tcctaccagg tctggggcgg catcccctac ggcgcgggag
    21241 attaccttac gcacgaagtg ctttcgctcg cgtatccggg ctatgttgac gcggggtact
    21301 accgagacga acggggcttc ctctccccaa caccgtgggg cgacatgatg gattgcctcc
    21361 tctcggatgc agcaccgtgg gttctgcggc aatacgggct catcgtcatt gcaggtgctt
    21421 tgtccgatga gaacgcggga actgaaaccc gcgacaagct ggacgcattt atggcgtcgg
    21481 gcgggcacgt ggtggcaacg gcggagaacg cgcgccgcct gtggccggaa ctcgggatcg
    21541 gggacgcaat ccgcgttccc gccggaacca ccgtgcggtt cgaacgaaca ggggaagtat
    21601 tcgaggaacc actggcgttt gacctgcaca ccatctccgc gatccctgac gaggtggtcg
    21661 ctgaggccgt gctggagcct ggttcgcagc ctgccgcagt ccggttcacc cgggggagcg
    21721 ggttgctgac cgtcgttctg tcccccttcg gcctcaacgc agaaccaacg gtgtccggcc
    21781 cggtccctaa tgaggcggag cgcgag
//
LOCUS       DAOSOO010000029        21491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2450_length_21491_cov_4.923820, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000029 DAOSOO010000000
VERSION     DAOSOO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 21491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21491
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2450_length_21491_cov_4.923820"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLT86_03680"
     CDS             <1..816
                     /locus_tag="PLT86_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HOT35749.1"
                     /translation="RSCVVLQRRFTEDEFVRAVEEDRVTQVSLVPTMLERVLERYPQW
                     RAPGYLRFALIGGGPANPDLPIRAVRRGIRTLLSYGLTECCSQVCTQPLDRSDDDPTC
                     GEPLDGFQVETRDSEIWVKGPAMMTGYYPQHGAPVFDKDGWFGTGDAGVLEDGQRLRV
                     LDRLDDAIVTGGEKVHPSEVEAILLTCPNVREACVFGRQDAVWGQIICAALTPAPGTS
                     VDSEDLRTTCARYLSPSRMPRKYCILDHIPLNRNGKHSRAETARVATPLLRDL"
     gene            complement(819..1235)
                     /locus_tag="PLT86_03685"
     CDS             complement(819..1235)
                     /locus_tag="PLT86_03685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT35750.1"
                     /translation="MYDDAVFVQERTVRIGDTDAAGIAYFASSLRLCHECYEALLEAR
                     NIGLREHFAPDGLALPIVHVEGDYKAPLRMGDRIRVMVTRCSLGNRSYTIEYAIVKES
                     GEIALTAKTVHVAVMLPDMKATAIPEALREALSKTD"
     gene            complement(1249..1917)
                     /locus_tag="PLT86_03690"
     CDS             complement(1249..1917)
                     /locus_tag="PLT86_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35751.1"
                     /translation="MGDLIRGATPVPVSDTCAQGLLQRLHLAPSTLNNQYTVTYAEEA
                     PLEGAGMRCRNISKYYQRRAGAKVLLVSQAPGHVGARWSGIPLTDTETIASGRLGEDY
                     EFSLVRTEPSSQAVHEGLGRHWGDVLLWSVVPWHPAGRCLDSNRKPSQIEVCAGIEVL
                     EYVLAHGEFRAIAALGEVASGALERLRVRHRVLPHPFYAGRSACIKGVRAFVGDVFGS
                     TATS"
     gene            complement(1978..3204)
                     /gene="rhaI"
                     /locus_tag="PLT86_03695"
     CDS             complement(1978..3204)
                     /gene="rhaI"
                     /locus_tag="PLT86_03695"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOT35752.1"
                     /translation="MEMSRDLYENLADSLTKKGVDVARVEAAIKSFEIETPSWGYGNS
                     GTRFGVFKEPGAARTLHERLQDAAEVHRVTGVCPSVAIHIPWDKVDDFAAVKAEAAAL
                     GVRIGAVNPNVFQDKAYKYGSLTNVDPAIRQKAMDAILECVDIMTEVDSRILSLWFAD
                     GSNYPGQVDMRKRKRWMQEALAHIHGKLPVGARMLVEYKLFEPGFYHTDICDWGMSLV
                     MCQHSGANAQVLVDLGHHAHGVNIEFIVASLLDEGMLGGFHFNNRKSADDDLTVGSIN
                     PFEVFLIFVEIVAAERSGLIKRPIAYMIDQSHIVKQKIEAMIQSVMNIQAAYARALLV
                     DWKALAHARSRNDAVDAERVIQDAYMTDVRPLLEKIRTDMGLEPDPILAFRNSGYLAK
                     IVEERKGELKGAASWG"
     gene            complement(3326..5254)
                     /locus_tag="PLT86_03700"
     CDS             complement(3326..5254)
                     /locus_tag="PLT86_03700"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT35753.1"
                     /translation="MSKKPVRIVENGKSNAVIVVAKDLGHQATKAVDDLVRVVALMSG
                     SAIPVVEDGAVQNPGAQIHIGQTAFVREADLLPASLPVNGYRIAVLESGGISRLVITG
                     RASLGISHGVYSLLTQELGVMWGMPAAMFEDIPKRQTVEIGHVDRTEFPSFGFRVFSG
                     NDPDWIRRNRIDDGGRNLPYYGHGHNLFTIFPPEKYAKDHPEYYALLDGKRQWPERQN
                     GEGPQPCLTNPDVIRITIETVRAFYDANPEISTLSLCPNDSAKFCECPSCLALDEGME
                     RYRGRRMNSDSYFYFIEAVAKELMKTHPDRYVGTYAYWTTELPPRRIKKLPPNVVVYL
                     TQDSSQYFDPAYEARDRELLESWSNVAHHLAVYDYYGLGWFTPRVYPTIVKRILPFLP
                     KVNVKGFYNETYPYWAHVAPHLFLVTRLLWDVHEDPDAVLTGWYERMFRECASQVRSY
                     FETLEAGWMKAREGKWFQGLDWLAEQLHQWDPAIRDEAWRKINEAYEAAKNETTRQRI
                     AYIRQGNRLAYLLSKTLEETLSLGPKDKQLEPRLRAIFGRVAEANTLFHNFIEADPTY
                     GHAYYRGARATIQMQWWMGFLASLIGEVLSENQRLKKKLTDEEPTYREMIAAEAYPDV
                     PRRIREARVMFGRPFPGS"
     gene            complement(5548..7935)
                     /locus_tag="PLT86_03705"
     CDS             complement(5548..7935)
                     /locus_tag="PLT86_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35754.1"
                     /translation="MKRRLERFWFVVSVAILLVAMEKPVEARRIDLKNVTIVQPAAAE
                     SVRTAVAARVLKEELEKRIGERVRLADAPPSRGVVFEISAQPSDSVSNGATLPERRPE
                     GFRVEIRTAGSNITTVQIVGADKRGALYGVGHVLRAVEWGPRGAWVPSEISAASAPKY
                     GFRGFQLGYRNTANSWDAWTPEQYEQYIRELVLFGANAIENIPIQDSDPSPHMKLSRE
                     EMNVRISRICQDYGIEYWVWVPADFDLADSAKKAEGLELFTRFCRRTPYLNGVFVPGG
                     DPGDNHPRLLLPHVRDLATILTQSHPNATVWMSLQGFNREKADYVYTYLQTYQPDWFG
                     GVINGPQSPLIQEDRARTPRKYAVRHYPDITHTVRCLYQTPQWDPAFAVTLGREPVNP
                     EPVYYALIHNAFAPFSHGFMAYSDGVHDDVNKVVFSVRAWDPEADVREILRDYTRFFF
                     GSAVSDLAADGILALERNWRGPVPTNGGIDATLALWQNLDRMAPENRGNWRWQMCQLR
                     ANYDAYVRQRLLYETSLENEANEWLLRAQVTGADSAAKMALRVLEKATTEPVRPELRA
                     RVEQLCDDLFRSIGLQTSVPKYQASGLERGAVLDFVDHPLNNRLWLQDQLAKVRSLTS
                     ETEKVRRLEEIARWENPGEGSFYDDIGHIAKQPHVVTGEGLSTDPMMIRNPNPGFWAW
                     DNNRSTWRPSLSTAMWSGHHQTGWPIALQYTNLDSGATFVLRVTGLGDAFPRANGERL
                     VPTLYGTRLGEFKEFPVPRHLTRGGELRITWDIPDEADVNWRNKSRVSEAWLLKR"
     gene            complement(8014..9354)
                     /locus_tag="PLT86_03710"
     CDS             complement(8014..9354)
                     /locus_tag="PLT86_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35755.1"
                     /translation="MVDVRPTPQQLAWQESELSMFLHFGMNTFTDREWGTGAEDPSQF
                     NPAKLDARQWARVAREAGFRYVILTAKHHDGFCLWPSRYTAHSVRNSPWADGNGDVIR
                     EFVDACSEFGLKAGLYVSPWDRHESSYGDSDAYNRFFVNQLTELLTNYGEIAEVWFDG
                     ACGEGPNGRRQSYDWQAFYETVRALQPEALIAICGPDIRWVGNEDGYAAETEWSVRTP
                     DPSIHGSGTDTVWWPAECDVSIRPGWFWHAAEDDKVKSLPQLVDIYLRSVGRNSVLLL
                     NVPPNRDGLLPDPDVRRLRELRGYLDRAFARNLAEQAETTPSWGHAGDPTSALTGSDT
                     QGWRAPTDSAEPVHIEFSFPSMVEFNTVVLRETIALGQFVERYRVEIQTAGGWREFST
                     GTTIGHKKIDKRARVTCGKLKVVIEKARGPARIQSVGIYDIPDLSDESVWGHQC"
     gene            complement(9441..10193)
                     /locus_tag="PLT86_03715"
     CDS             complement(9441..10193)
                     /locus_tag="PLT86_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT35756.1"
                     /translation="MKAFLEWLGGISTFFGRTVAAAVRPPYEFREFVRQLDEIGTRSL
                     PLVGAAGAAIGVVLAMQTRSTLARFGADSLLPSMIAIAVVRELGPVITGLMVAGRIGA
                     GLAAEIGAMRVTEQIEALEVSAINPFRYLIAPRIAACIVAMPLLTAYCDFLALAGGYG
                     AASITMQTSVRLYFDASMQILEFVDVVPSLAKTAVFGFLIGTIGCYHGYSTRGGTAGV
                     GRAATFSVVLSSLMVILSDVLLVRISQTLFGG"
     gene            10349..11167
                     /locus_tag="PLT86_03720"
     CDS             10349..11167
                     /locus_tag="PLT86_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT35757.1"
                     /translation="MELRRHLVDKLRALTADFAYDGRDGAHQPLRSVPTRFRGHLERG
                     REWAGASGNCWVYTEDLRSTSRAANDLLPRLERACTENEQLAASFDAKIAALDIETCG
                     FTSAPVFMVGILTAGAETLSFHQAVARDYEEEPALLEWTRARLHDVGLVVTFNGTTFD
                     LPFLRERFRFHRLPELPQMRHLDLLPVCRRIWKGQFPNCRLQTLEERILGFVRKGDIP
                     GVEIPDVYHAFVSTGDSRHICRVAEHNRLDLISLLRILVEATRVRPKHEDDISR"
     gene            11164..12612
                     /gene="murD"
                     /locus_tag="PLT86_03725"
     CDS             11164..12612
                     /gene="murD"
                     /locus_tag="PLT86_03725"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT35758.1"
                     /translation="MKHYEPGQTWKGARVTVMGLGLFGGGVGAARFAVQQGAYVTVTD
                     LRPAEQLAESLDALRGLPIRYVLGRHEEPDFEGADIVIASPAVPLQSPFLDRARNAGA
                     HLTTEILLFAERCRGNVIAVTGSNGKTTTTSLIGAILSLHDTRTVTGGNLGRSLLEIV
                     DQIAPGDPVVLELSSFQLEWLGPAHWYPHVAVITNLTPNHLDRHRTFASYVEAKRQIV
                     AYQPAEAHAVLNARDENVRAMTSATSAHVHWFDSADPVQDGTWLEKGDIVCSIGGRAH
                     HVMPASEVPLPGAHNLENVLAAVAATEIMGVQVPAIRKAVGSFRPVEHRLELVRTVNG
                     VRYFNDSIATTPESAICALRAFPERTLLLIAGGYDKKIPFDSLGAEIAERAAALITIG
                     TTSDKIRKAAVRAGFPEERIFPAGTLETAVQTAFSLAQPGQSVSLSPACASYDQFRNF
                     EERGRMFKKLVLELPENPVSRRDRRGKMNNSS"
     gene            12815..13900
                     /locus_tag="PLT86_03730"
     CDS             12815..13900
                     /locus_tag="PLT86_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOT35759.1"
                     /translation="MGEVRVAIIGAGNCASSLVQGVHYYQNASEDEFVPGLMHVNLGG
                     YHIKDIKFVAAFDIDKNKVGKDLSEALVSPPNNTIQFCQVPNLGVKVHRGMTHDGLGH
                     YLSEIIEKAPGPTDDIVKILKETKTDVVVNYLPVGSEEATKWYVEQVLAAGCGFVNCI
                     PVFIASQEYWQKRFEEKGLPVIGDDIKSQVGATITHRVLTTLFRDRGVRLEETSQLNV
                     GGNTDFLNMLERSRLQSKKISKTNAVTSMLDYDIGKDNVYIGPSDYVAWLKDRKWCYI
                     SMNGRTFGDVPLKLELKLEVWDSPNSAGVVIDAIRCCKLGLDNNLSGALIEPSAYFKK
                     TPPKQFPDSVARQNLEAWIKKYGNKKS"
     gene            13983..14915
                     /gene="rbsK"
                     /locus_tag="PLT86_03735"
     CDS             13983..14915
                     /gene="rbsK"
                     /locus_tag="PLT86_03735"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOT35760.1"
                     /translation="MENTSPQIVVVGSSNTDLVVRVPELPRPGETLLGLDFQIHAGGK
                     GANQAVAARRLGATVTLIANIGVDSFGEASIERFRAEGIRVGAIARDTEAASGVAMIW
                     VSETGQNSIVVALGSNARLTPQHVEDASGVFQNADVLLTQLETPLPTVAFALRTARSI
                     GVRTILNPAPATVLGPELLADVDWITPNETEAARISGIEVQNVSDAEHAANALRRMGA
                     RNVVVTMGEAGALLMTDDESLHVPARRVPAVDTVAAGDAFNGAFAVALARNAPPREAV
                     EFACAAASLSVTRRGAQPALPTADEVTKFASQLA"
     gene            complement(14935..16221)
                     /locus_tag="PLT86_03740"
     CDS             complement(14935..16221)
                     /locus_tag="PLT86_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35761.1"
                     /translation="MTGVSSPQITTHDRQVLRDLAKEIRDIAHSDENLEKKDAWYAHN
                     DLKPRRPLVLIESFPATDELVTDDILSCRDDWARKQEKHLRTTIYHFRNIRDDDVIEP
                     YLTWNWHVSAGNYGVTSTRHDSDKIQRVDGKVVGSYSWEAPVKDLDADFGKLKPRVFS
                     VDRESSLLERDRLEDVFGDILGVRQRGGYWWTTGMTIIAIDLIGLENLMLAMYDNPAG
                     LHRLMGFLRDDHIAFAEWLEKEGLLSLNNENDYIGSGSRGYTRELPQKDWKPGGKVRT
                     RDCWVLSESQETVGVGPGQFEEFIFPYQKDVVERFGLCYYGCCEPVHSRWHVIKKFSN
                     LRSVSVSPWCDEEMMGEALAGKYVYSRKPNPTLISTDVFDENAIRNDVAKTLRCARKC
                     SVEIVMKDVHTLAGDPMRAARWVELARQAIDLEWGK"
     gene            complement(16258..17355)
                     /locus_tag="PLT86_03745"
     CDS             complement(16258..17355)
                     /locus_tag="PLT86_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35762.1"
                     /translation="MTDRERVLAIMHHQPVDGVVWQPRIDHWYYTNKRAGTLPERYRD
                     MSLYEVYVDLDCSIRGYEFYNPCISRVYDSSVSFRVVEDTPHRRVRLWETPLGVLTEV
                     EGKTAESSLLTEFPVKSIADLRIVRYTLEALNFEFSEERFWEAEKLVGDRGVGSVYNL
                     RTPLMRLFIEFMGFENTIVALHEFPEEMDSFIRFLEEDDQKYIDVIKKSPLPLLNFGD
                     NVDSNMLPPSLMRKYALPYYRRRTAELRAAGKASYPHWDGALKGLLPFVRECGFDGYE
                     AFTPTPMGDVSLDEIAEAMGHDYVLIDGIPATHFIPPFTRDDVEEFATLVLQTFAPSL
                     ILGISDELPPSGDIESVRRVAEIVRSFRISK"
     gene            17498..18466
                     /locus_tag="PLT86_03750"
     CDS             17498..18466
                     /locus_tag="PLT86_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOT35763.1"
                     /translation="MHSPEHPHKSDSQIPSTAVKGRIFDVQTFSIHDGPGIRTTVFLK
                     GCPLRCVWCHNPESMETEPDVGFIPAKCIACGQCTLVCTHEGHEIVDGRHVFQRGLCV
                     RCGSCTTECPTGALELAGKEVTAAWVMSEVEKDRMFYERSGGGITLSGGEPLMQPEFS
                     AALLRLARDSRLHTAVDTSGYAGETVFDRVTALADLVLFDIKMIDERAHRRLTGVSNR
                     RILRNLQRLLRDENGPKVWLRLPVIPGQNDSPDAIQNLIDFCASLWGNPRLEAIHLMP
                     YHRLAESKYQEFGKRYLINGTHPPADEDISAIIRRFSERGVPVTRS"
     gene            18496..20862
                     /locus_tag="PLT86_03755"
     CDS             18496..20862
                     /locus_tag="PLT86_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35764.1"
                     /translation="MPTLGRDEEVIQGAYGIDLSPRVERLRDYVLNDATWADHYAPRA
                     WLVAESMKQTEGQPPLFRQAAALAHVIRNYPVTIREGELILGYQQHESFRGIGWLEFD
                     NLTNGERREALRNHVQNLSIPDEWKQKVFDAIDYWREHPRVIPHFWPQTPDYISRDTR
                     AGVYFGGGGSEGHTVPGYERVLRHGFHAILEQIDGEIARLQPWKPDDQKRKLWLHSVR
                     LIAAAACELGKRYAEELRALATRESDPLQAEEYRRLADICERVPARGARTFHEAVQSL
                     WFCHLIMGWEDGINANSFGRYDQYLYPFYAADREEGRITEDEALELLEAFHLALYKSY
                     DVQQATIGGQNPDGTDATNELTYLFLEAMWRVNLIRCLSVRVHRGTPRRLLESAFRLI
                     RRGGGVPFFFNDEAIVPALVEKGIPVEDARQYAIIGCVEVTIPGKTNPHAVSHMMNVA
                     KCLELALNDGKDLATGEQIGPQTGTLADMPDIEALWNAYKLQLEHFARHAVELSNQGD
                     LAQPFRFPLPYTSILTDDCIARGKDMTDGGAHYHYHSTCAIGLPNVGDSLYAVDQLVF
                     KEKSVSPDELLEALKANWEGEEREKLRQKVIHRLAKYGNGNGDVDRWVERAAHHYCDH
                     MATYTTPHGGSFHAHLFSFVWHLDCGRATNATPDGRKAHDPLAYSVSPQQGRDVEGLT
                     ALMRSLMTLPHEHAAGSSSAIIEIAPKLLDEVSLDKVLDVVQTAIDKGVGQMQFNVID
                     SATLRRAQENPEAYSHIAVRVSGFSMRFCLLDKTMQDHIIARTKHEEM"
     gene            20887..>21491
                     /locus_tag="PLT86_03760"
     CDS             20887..>21491
                     /locus_tag="PLT86_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT35765.1"
                     /translation="MIQPILRTERIARLRKWQFDQRGGQVDREMLWHRYMAGHQSLPI
                     DARYAGVFAYLMDNLPVEINDDELIVGRIKRREMTPEEKAERERIAGSTFGDPLGDIL
                     KKLISPDAHESVAEGRHVAGAMTGHMTPDYPAVLRKGLNGIRREVRERLTRATTEKER
                     AFCHAALEVLSGAMRMARRYATHARELAATATPERREELLR"
BASE COUNT         4596 a         6557 c         5909 g         4429 t
ORIGIN      
        1 cggtcctgtg tcgttttgca acgacgcttc accgaagacg agttcgtgcg tgccgtagag
       61 gaagatagag tgactcaggt gtccctcgta ccaacgatgc tggaacgggt gctggagcgt
      121 taccctcagt ggcgcgcgcc cggatacctt cgatttgctc tcatcggcgg ggggccggcg
      181 aatcctgacc tccccatacg agccgtccgg cggggtatca gaacgctcct ttcctacgga
      241 ctcaccgaat gctgttcgca ggtatgcacg caaccccttg accgttccga tgatgatccg
      301 acctgcggcg aacctctgga tggtttccag gtagaaacca gagactccga gatatgggta
      361 aagggccctg cgatgatgac cggatactat ccacagcacg gcgcacctgt gtttgacaaa
      421 gatggctggt ttggtactgg cgacgctggc gttttggagg atgggcaacg cctccgcgta
      481 cttgacagac tggacgacgc catcgtgacc ggcggcgaga aggtgcatcc ttccgaagtg
      541 gaggcgatcc ttctgacgtg cccgaatgtg cgtgaagcgt gcgtctttgg tcgtcaggac
      601 gccgtatggg ggcaaatcat atgcgcagcc ctgacaccgg ctccgggaac cagtgttgac
      661 tcggaagact taagaacgac gtgcgcgagg tatctttccc cgtccagaat gccgaggaaa
      721 tattgcatcc tggaccacat ccccctcaac cgcaacggaa aacacagccg cgccgaaacc
      781 gcacgcgtcg cgactccgct cctccgcgac ctgtagactc agtcggtttt cgagagcgcc
      841 tcgcgcaacg cctccggaat tgcggtagcc ttcatgtcgg gcaacatgac cgcgacatgc
      901 accgttttcg cagtaagcgc aatttcgccg gattccttga ctatcgcgta ttcgatcgtg
      961 taggaacggt ttccaagtga gcaccttgtt accatcactc gaatccggtc gcccatcctc
     1021 aaaggagctt tgtagtctcc ttccacgtgc acgatcggaa gggccaatcc gtccggtgcg
     1081 aaatgttccc gcagccctat gtttctggct tccagcagag cttcgtagca ctcatgacac
     1141 aaccgcagtg acgacgcgaa atacgcgatg ccggctgcgt cggtgtcgcc tatgcgcacg
     1201 gtccgttcct gaacgaatac cgcgtcgtca tacatccctg atcgtccctc acgatgttgc
     1261 ggtggaaccg aacacatcac ccacaaacgc ccgtacccct ttgatgcacg cactccggcc
     1321 cgcgtaaaag ggatgcggca aaacacggtg acgtacacgg agcctttcca gtgctcctga
     1381 cgctacctct ccaagcgccg cgatagcccg gaactcacca tgtgcgagca cgtattccag
     1441 aacttcgatt cccgcgcaaa cctcgatctg cgacggtttc cggttggagt ccaggcaccg
     1501 gccggcggga tgccagggta cgaccgacca gagaagcacg tccccccagt gtcggcccaa
     1561 accttcatgc actgcctgag aggagggctc ggtacggacg aggctgaact cgtagtcttc
     1621 acccaggcgg cctgacgcga tagtttcggt gtccgtgagc ggaatacccg accaccgggc
     1681 ccccacatgc ccgggcgcct ggctcacgag gagtaccttc gcacccgctc gccgctgata
     1741 atacttcgag atgttccggc agcgcatccc ggccccctca agcggagcct cttcggcgta
     1801 cgttaccgtg tactgattgt tcaaagtcga aggtgcaagg tgcaagcgct ggagcagacc
     1861 ttgcgcgcag gtatccgaca ccggcacagg ggtcgccccc cgtatcaaat cgcccacagc
     1921 ttgttctctg ccaccaaaac aatgctgatc gcctcaggtg ccgtgtgcgg aaacatatca
     1981 gccccagctt gcggcaccct taagttcgcc cttccgctct tccacgatct tcgccaggta
     2041 ccctgagttg cgaaacgcga gaatgggatc gggttccaga cccatatccg tacgaatctt
     2101 ctccagcagg gggcgcacat ccgtcatgta ggcgtcctgt atcacccgtt cggcatccac
     2161 cgcatcgttg cgcgagcggg cgtgagccag cgccttccaa tccaccagaa gagcgcgtgc
     2221 atatgcggcc tggatattca tcaccgactg aatcatcgcc tcaatttttt gcttgacgat
     2281 gtggctctgg tctatcatgt aggcaattgg gcgcttgatc aatcccgagc gctccgccgc
     2341 aacaatctca acaaaaatca ggaacacctc aaacgggttg atggaaccca ccgtaaggtc
     2401 atcgtcagcg cttttgcgat tgttgaagtg gaacccgcca agcatccctt catcgagaag
     2461 cgatgcgact ataaattcga tgttgacgcc atgcgcatgg tgccccagat ccacaaggac
     2521 ctgagcgttc gccccgctgt gctggcacat taccagagac ataccccagt cgcagatgtc
     2581 ggtatggtag aatccaggct caaagagctt gtactccacg agcattcgtg cccccacggg
     2641 cagtttcccg tgtatgtgcg ccaacgcctc ctgcatccag cgcttccgtt tgcgcatgtc
     2701 cacctgtccg gggtaattcg atccgtcagc aaaccagaga ctcagaatcc gtgaatccac
     2761 ctccgtcatg atatccacgc attcaagtat ggcgtccatc gccttttgtc ggatcgccgg
     2821 atcaacgttg gtgagactcc cgtacttgta cgccttgtcc tgaaacacat tggggttgac
     2881 cgcgccgatt cgcactccga gcgcagccgc ttcggctttg acggcagcaa agtcgtcaac
     2941 cttatcccat ggaatgtgaa tcgcaaccga cggacacacg cctgtgacgc ggtgaacctc
     3001 cgccgcatcc tgaagccgtt cgtgcaaagt tctcgcagca cccggctctt tgaatacgcc
     3061 aaagcgcgta cccgaattac cgtaccccca cgacggcgtc tcgatttcga acgactttat
     3121 cgccgcctca acccgcgcga cgtcgacacc ctttttggtc aacgaatcgg ccagattctc
     3181 gtaaagatct cggctcattt ccatacccct ttcatcgcgc ccacatcaaa atgaccagac
     3241 aaatgcgttc agccaactgc agcgcgcatt ccctcacccc aaagcttcgg ggccaggcta
     3301 cccgccacgt cacgatatac tcccatcaac ttccgggaaa gggccttccg aacatcaccc
     3361 ttgcctccct gatgcgccgc gggacatccg ggtacgcctc cgccgcaatc atctcccggt
     3421 aggtcggctc ctcgtcggtc agtttctttt tgagacgctg gttctcagac agcacctcgc
     3481 caatcaagga cgcgaggaag cccatccacc actgcatctg aatcgtcgcc ctggcaccgc
     3541 ggtaatacgc gtgaccatag gttggatccg cttcgatgaa attgtgaaac aacgtatttg
     3601 cttcggccac cctgccgaaa atggcccgca gacgtggctc caattgctta tccttcggtc
     3661 ccagggaaag cgtttcctcg agcgttttgg aaagcaggta tgcaaggcgg ttgccctgtc
     3721 ggatgtaggc tatccgctgc cgcgtcgtct cgttcttggc ggcttcatac gcttcattga
     3781 ttttgcgcca cgcttcatca cggatcgcgg gatcccactg gtgcaattgc tccgccaacc
     3841 agtccaggcc ttggaaccac ttcccttctc gcgctttcat ccaccccgct tccagggtct
     3901 cgaaatagga ccgaacctgg gaggcacatt cccggaacat ccgttcatac cacccggtca
     3961 gcacggcatc cgggtcttcg tgaacgtccc aaagaaggcg cgtcaccaga aacaaatgcg
     4021 gagcaacatg agcccagtac ggatacgttt cgttgtagaa ccctttgaca ttgactttcg
     4081 gcaggaacgg caaaatgcgt tttacaatcg tcggatagac cctcggcgtg aaccatccca
     4141 ggccgtaata gtcgtacacc gccagatggt gggcgacgtt cgaccaggat tccagcaact
     4201 cccggtcacg cgcttcatag gcgggatcaa agtactggct cgaatcctgc gtaaggtaca
     4261 cgacaacgtt cggcgggagc ttcttgattc gccttggagg cagttccgtc gtccagtacg
     4321 cgtaggtccc cacgtatctg tctgggtgtg tcttcatgag ctctttcgca acagcctcga
     4381 tgaaatagaa gtaactgtcc gaattcatgc gcctgccacg atagcgttcc atcccttcgt
     4441 caagagcgag acaggacgga cactcgcaga acttcgcgct gtcattcggg cagaggctga
     4501 gtgtgctgat ttccgggttg gcatcgtaga acgcccgcac cgtctcgatc gtaattctga
     4561 tcacatcggg gttcgtgagg catggttgcg gcccctctcc attctgccgc tccggccatt
     4621 gccttttgcc gtccagcaac gcgtaatact ccggatggtc tttcgcgtat ttctccggcg
     4681 gaaaaatcgt gaaaagattg tgtccgtgcc cgtaatatgg cagattcctt cccccgtcat
     4741 ctatgcgatt ccttcgaatc cagtcgggat cgtttccact gaaaacacgg aaaccgaacg
     4801 acgggaactc cgtccggtcg acatgaccta tttccacggt ctgccgcttc gggatgtcct
     4861 cgaacatcgc cgccggcatc ccccacatta ctccgagttc ctgcgtgagc agggagtaca
     4921 cgccgtgcga aatgccaaga gaagcgcgac cggtgataac cagtctcgaa atcccccccg
     4981 attcgagcac cgcgatccga tatccattca ccggaagact agcgggcaac aggtccgcct
     5041 cccgcacaaa cgccgtctgt ccgatgtgga tctgcgcacc cgggttttga acggctccgt
     5101 cttccaccac cggaatcgcc gagccactca tcagcgcaac aacgcgcacg agatcatcca
     5161 ccgccttcgt cgcctgatgc ccgagatctt tcgccaccac gattaccgcg ttcgatttcc
     5221 cattttccac gatacgcaca ggtttttttg acatcataaa tctcccggca tgcgtccaga
     5281 tccctgttgt gtttcagact caccgcaact cataacggca aaaacacgca gaggcacccg
     5341 ccatcctggt tccactcaga gaaacacctt tgatatcctc ggcacaagtt cgcaccattt
     5401 tcaaggtaat gattagctga attacacctc acccagtcct tattatatgc gttactgcgc
     5461 atatttgtat attatccaat gttcggtcgt tttcagacaa tcttgttgcc gccattccga
     5521 gattcggctc ccagaaccga gcacttctca acgcttcaac aaccatgctt ccgaaacgcg
     5581 cgacttgttc ctccagttga cgtcggcctc atccggaata tcccaggtta tccgaagttc
     5641 cccacccctt gtgaggtgcc gcggaaccgg aaattccttg aactccccga gacgggttcc
     5701 gtaaagagtc gggacgaggc gctcgccatt cgcacgagga aacgcatcac ccaagcccgt
     5761 aacacggagc acgaacgtcg cgcccgaatc cagattcgtg tattgcaggg cgataggcca
     5821 gcccgtttgg tggtgaccgg accacatcgc cgttgaaagg gaggggcgcc aggttgaacg
     5881 gttgttgtcc cacgcccaga agccggggtt cgggttcctg atcatcatcg gatccgtaga
     5941 aagcccctcg cccgtaacca cgtgaggctg tttcgcgatg tgaccaatgt cgtcataaaa
     6001 actcccctca ccagggtttt cccaccgagc gatttcctcc agtcgccgga ccttctctgt
     6061 ttccgaggtc agactccgaa ccttcgcgag ctggtcttgg agccacaacc tgttgttgag
     6121 cggatgatcc acgaaatcca gcaccgcacc gcgctccaaa ccggatgcct gatacttcgg
     6181 gacgcttgtc tggagcccga tcgagcggaa aaggtcatca cagagctgct ccacacgtgc
     6241 cctgagttcg ggtcgaacag gttcggtggt cgccttttcc aaaactctca atgccatttt
     6301 tgcggcggaa tcagcgcccg ttacctgtgc acgcagaagc cactcgttcg cttcgttttc
     6361 caggctcgtt tcatacagga gcctttgtcg gacgtacgca tcgtaattcg cccgcaactg
     6421 acacatctgc cagcgccagt tgcccctgtt ctccggcgcc attcgatcca ggttctgcca
     6481 aagcgccagt gtggcatcaa tacccccgtt tgtcggaacg gggccacgcc agttcctctc
     6541 cagcgcaagg atcccgtctg cggcgaggtc agacaccgcg gacccgaaaa agaagcgcgt
     6601 gtagtcgcgg agaatctctc tcacgtccgc ttcggggtcc catgcccgca cgctgaacac
     6661 caccttgttg acatcgtcgt gaacgccgtc ggagtacgcc atgaagccat gcgaaaacgg
     6721 ggcgaacgca ttgtgaatca gcgcataata caccggttcg ggattcaccg gctctcttcc
     6781 gagagtaacc gcgaacgccg gatcccattg aggcgtctgg taaagacacc gaaccgtatg
     6841 tgtgatgtct ggataatggc gaaccgcata tttccttggc gttctggccc gatcctcctg
     6901 gatcagaggg ctctgcgggc cgttgatcac gccgccgaac caatccggct ggtaagtttg
     6961 caaatatgtg tagacgtaat ctgccttttc gcgattgaac ccctgcagcg acatccacac
     7021 cgtcgcattc gggtgagatt gcgtcagaat cgttgcgagg tcgcgcacgt ggggaagaag
     7081 caaccgcgga tggttgtctc ctggatctcc gccgggcaca aacactccgt tcaggtacgg
     7141 cgtccttcgg cagaagcgcg tgaacagctc caatccttcc gcctttttcg ccgagtccgc
     7201 gaggtcgaaa tctgccggta cccacaccca gtattcgatg ccgtagtcct ggcagattct
     7261 gcttattcgc acattcattt cttcgcgcga aagcttcatg tgcgggctcg gatccgaatc
     7321 ctggatggga atgttctcga tggcgttggc accaaaaaga accaactcgc ggatgtactg
     7381 ctcatattgt tccggcgtcc acgcgtccca actgttcgcg gtattccggt acccgagttg
     7441 gaatcccctg aatccgtatt tcggtgccga tgccgccgag atctcgctcg gaacccacgc
     7501 cccacggggg ccccattcca cagcccgcag aacatgcccg acgccataca gagcccctcg
     7561 tttgtccgct cccacgatct gcaccgtagt gatattcgag ccagcagttc tgatttccac
     7621 ccggaatcct tccggacgcc gttcgggcaa tgtcgcaccg ttcgaaacac tgtccgacgg
     7681 ttgggcggat atctcgaaaa cgacgcccct ggaaggtggc gcatccgcca accgcacacg
     7741 ctcgccgatg cgcttctcaa gctcctcttt cagaacccgc gccgccacgg cagtacgcac
     7801 tgattccgcg gcagccggct gaactatcgt aacgtttttc aggtcaatcc gtcgcgcttc
     7861 aacgggtttc tccattgcga ccaggagaat ggcaacggat acgacaaacc agaaccgttc
     7921 gagcctcctc ttcatcgttt tcatcccctt atgacttcta tggttccgat gtactccgcg
     7981 cagcgcgatt cagacaacat cagcaccgca aaactaacac tggtgtcccc aaaccgactc
     8041 atcgctcaaa tccggaatgt cgtagatgcc cacgctctga atccgtgcgg ggccgcgcgc
     8101 cttttcgatg accaccttca gctttccgca ggttacgcgt gcacgtttgt ctattttttt
     8161 gtgaccgata gtcgttccgg tggaaaactc ccgccagccg cccgctgtct gaatttccac
     8221 gcgataacgc tctacgaact ggcccagggc gattgtctcc cgcaacacga ccgtgttgaa
     8281 ctccaccatg gagggaaacg agaactcaat gtgcacaggc tccgcggagt ctgttggcgc
     8341 ccgccaccct tgcgtatcag aacccgtcaa tgcggaagtt gggtcgccag cgtgtcccca
     8401 cgaaggcgtg gtctccgcct gttccgcgag gtttctcgcg aacgcgcgat caagataccc
     8461 ccggagttct ctcaacctgc gcacgtccgg atccggcagg agcccgtctc tattgggggg
     8521 aacgttcaaa agcagaacgg aattcctgcc cacggacctc aggtagatat ccacgagctg
     8581 cgggagagac ttgaccttgt cgtcctccgc ggcgtgccaa aaccatcccg gtcggatcga
     8641 cacgtcgcac tccgccggcc accatactgt gtcggttcca ctcccgtgaa tcgaagggtc
     8701 cggtgttcga acgctccatt cggtttcggc ggcatagccg tcctcgtttc cgacccagcg
     8761 tatgtccggg ccgcagatgg ctatgagggc ttccggctgc agagctcgaa cagtctcgta
     8821 aaatgcctgc cagtcgtacg actgcctcct gccgttcgga ccttcgccgc aggcaccgtc
     8881 aaaccagacc tccgcaatct ccccgtagtt tgtgagtaac tcagtcagtt ggttcacaaa
     8941 aaaacggtta taggcgtcag aatcgccgta cgacgattcg tgacgatccc atggtgacac
     9001 atacaaccct gccttcaggc cgaactccga acacgcgtcc acgaactccc tgattacgtc
     9061 accgttgcca tcggcccaag ggctgttcct gaccgaatga gccgtatacc gactcggcca
     9121 gagacagaag ccgtcatggt gcttggccgt aagtatcaca taacggaaac ctgcttcgcg
     9181 ggctactcgc gcccattgtc gggcgtccag tttcgcagga ttgaactgcg aaggatcttc
     9241 agcgcccgtt ccccattctc tgtcggtgaa cgtattcatc ccgaaatgga ggaacattga
     9301 aagctcagat tcctgccacg caagctgctg cggcgtcggc cgaacatcca ccataaccgt
     9361 tccttttctc gcttgatctg cgaatcacct caaccgcgaa cgccacgcgg gtacgcgctg
     9421 cggctctgtg cactcagtgt tcagccgccg aacagcgtct ggctgatcct taccagcaga
     9481 acgtcggaaa gaatcaccat aagggaagac agcaccaccg aaaacgtggc tgcccgaccg
     9541 acgccggcgg taccgccgcg ggttgaatac ccgtgatagc acccgatcgt gccgatcaga
     9601 aaaccaaaca ctgcggtctt ggcaaggctc ggcaccacgt ccacgaattc gaggatctgc
     9661 atgctcgcat cgaagtagag ccgaacggag gtctgcatgg tgatggatgc ggcgccatag
     9721 cctcccgcca gcgcaagaaa gtcgcaatac gcggtgagga gaggcatcgc gacgatgcac
     9781 gcggcgatgc gcggcgcaat cagataacga aacgggttga tcgcggagac ctccagagct
     9841 tcgatctgct cggtaacccg catggcgccg atttctgcgg ccagtcccgc accaatccgc
     9901 ccggcaacca tcaacccggt aatcaccggc ccgagttcgc gcaccacggc gatggcaatc
     9961 atcgacggaa ggagcgaatc tgcaccaaaa cgcgcaagcg tggaccgggt ctgcatcgca
    10021 agtaccaccc cgatcgccgc gccggccgca cctaccagcg gtaacgaccg agtgccgatc
    10081 tcgtccagtt gccgtacaaa ctcgcggaat tcatagggag gtcgcaccgc cgcggcgacc
    10141 gtccttccga aaaatgtgga aattccaccc aaccactcaa ggaaggcttt catgtcttcg
    10201 ttctccccgg aaatagaacc gcgaccccat gaagtacctg aattgtgtct ccgcattgca
    10261 atgcctcgca tcccgctgac tggagttgag gatcgaccgt atctttcctt gttgtcctca
    10321 acagcgcacg caccaccaga ttggttcgat ggaactccgc agacacctcg tggacaaact
    10381 tcgcgcgttg acggccgatt ttgcgtacga cggccgcgac ggagcacacc agccgctgcg
    10441 ctccgttcca acgcgttttc gcggtcacct cgaacgtggc agggaatggg ctggtgcctc
    10501 ggggaattgc tgggtgtaca cggaggacct gcgatccact tcccgtgcgg cgaatgactt
    10561 gttgccacgg ctggaacgcg cgtgcaccga aaacgagcaa ctcgccgcgt cgtttgatgc
    10621 aaaaatcgct gcgcttgaca tcgagacgtg cggtttcacc tccgcaccgg tgttcatggt
    10681 cggaattctg acagccggcg cggaaacgtt atcctttcac caggcggttg cgcgcgacta
    10741 cgaagaagaa ccggcgttgt tggaatggac gcgcgcccgg ttgcatgacg tcgggttggt
    10801 ggtgacgttc aacggaacca cctttgacct gcccttcctt cgtgaacggt ttcgttttca
    10861 ccgcctcccc gagctgcctc agatgaggca tctggatttg ttgcctgttt gccgccgcat
    10921 ctggaaaggg caattcccca actgccggct gcagacgctg gaggaacgta tcctcgggtt
    10981 cgtcagaaag ggagacatac cgggtgtgga aatcccggac gtataccacg cgtttgtcag
    11041 cactggcgat tcgaggcaca tttgtcgcgt cgcggagcac aatcggctcg accttatctc
    11101 gctcctgcga attcttgtgg aagccacaag ggtccgacca aaacacgagg acgacatttc
    11161 ccgatgaagc attacgaacc cggtcagacg tggaaaggtg cacgagttac cgtgatgggg
    11221 cttggcctgt tcggcggcgg tgtcggagcc gctcgattcg ccgttcagca gggcgcgtac
    11281 gttactgtca ccgacctgag gccggcagag cagctcgcgg aatctcttga tgccctgcgt
    11341 ggtctcccga tccggtatgt actcggccgc cacgaggagc ccgattttga gggtgccgac
    11401 atcgtcatcg caagcccggc tgttcctctg cagtccccgt ttctggatcg cgcgaggaat
    11461 gccggagcgc atctcacgac agagatcctc cttttcgcag agcgttgccg gggaaacgtg
    11521 attgcagtga ccggatccaa cggaaaaaca accacgacga gtctgatcgg ggccatcctc
    11581 tcactccatg acacgcggac agtgaccggc gggaaccttg gcagatcgtt gctggaaatt
    11641 gtcgatcaga tcgcaccggg ggacccggtc gttctcgagt tgagcagctt ccagcttgaa
    11701 tggctggggc cggcacactg gtatcctcac gtggcagtga ttaccaacct taccccgaac
    11761 catctggatc ggcaccgaac gttcgcgtcc tacgtcgaag cgaagcgcca gattgtggcg
    11821 taccagccag cagaagcgca cgcagtactc aacgcacggg acgaaaacgt tcgggcaatg
    11881 acaagcgcga cctccgctca cgttcactgg ttcgacagcg ccgaccccgt gcaggatggg
    11941 acctggctcg aaaaaggaga catagtctgc tcgatcggag ggcgcgcgca tcatgtcatg
    12001 ccggcttctg aagtgccgct gcccggcgcg cataacctcg aaaacgtcct tgcggcggtc
    12061 gccgcgacag aaatcatggg agtccaggtg cctgcaatcc ggaaggcggt cggctcgttc
    12121 cgcccggttg aacaccgcct ggaactggtt cgaaccgtca atggagttcg ctatttcaac
    12181 gattcgattg ctacgactcc ggaatcggca atatgtgcgt tgcgagcctt tccggaaagg
    12241 actctgctcc tgattgccgg cgggtacgac aaaaagattc catttgacag tcttggagcc
    12301 gaaattgcgg agcgcgcggc agcacttatc accataggca caacctccga caaaatccgg
    12361 aaagcagccg tgcgggcggg cttcccggaa gagcgcatct tccctgcggg cactctcgaa
    12421 acggcagtgc aaacggcatt ttcactcgcg caacccgggc agtctgtttc cctctctccg
    12481 gcatgtgcaa gctacgacca gttcaggaat ttcgaagagc ggggccggat gttcaaaaag
    12541 ctcgttcttg aattgccgga gaatccggtt tcgagacgag accgacgcgg caagatgaac
    12601 aattcttcat gacacattca cagaccattc atgtgcgttc tgtatgcttt tttccagaaa
    12661 tttgtgaagc cgcgagctct gcttgcatcc agcgagccgc ttccattcaa gattcaaatg
    12721 caacgctttt tgctgcaccg cacgctgagc acagcacctc aacgttcgtc tcgaataccg
    12781 gcggcgcagg ttcgtaaaaa aaggagatca acacatgggt gaggtacgcg tcgcgatcat
    12841 aggagccggg aactgcgcct cttcgcttgt tcaaggtgtg cattactacc agaatgcctc
    12901 ggaagacgag tttgtccccg gtctgatgca tgtcaatctg ggtggttacc acataaagga
    12961 catcaagttc gtcgcggctt ttgacattga caaaaacaag gtcgggaagg atctctcgga
    13021 ggcactcgtt tcgccgccca acaacaccat tcagttctgc caggtcccaa acctcggcgt
    13081 caaagtgcac cgcggaatga cgcacgacgg cctcggacat tatctgtccg aaataatcga
    13141 aaaagcgccg ggccccacgg atgacatcgt caagattctc aaagagacga aaacggacgt
    13201 ggtggtgaac tatctgccgg tgggttccga agaagcaacg aaatggtatg tggaacaggt
    13261 tctggcggcg ggctgtggct ttgtgaactg cattccggtg ttcatagcat cgcaggagta
    13321 ctggcagaaa cggtttgagg aaaagggcct tcccgtcatc ggggatgaca tcaagtcgca
    13381 ggttggagcc accatcaccc accgtgtact gacgacgctc ttccgggatc gcggagtacg
    13441 tctggaggaa acaagccagt tgaacgtggg cggtaacacc gattttctga acatgcttga
    13501 gcgttcgcgt ctgcaatcga agaaaatcag caaaaccaac gcagtgacgt ccatgctcga
    13561 ttacgacatc ggtaaggaca atgtctacat cgggccgtcg gattacgtcg cgtggctgaa
    13621 ggacaggaag tggtgctaca tcagcatgaa cggcagaacc ttcggtgatg tcccgctcaa
    13681 actcgagctg aaactcgagg tgtgggattc acccaattcc gcaggtgtag tgattgacgc
    13741 cattcggtgc tgcaagcttg ggcttgataa caacttgagc ggggctctga ttgaaccgag
    13801 cgcctatttc aagaagacgc cgccgaagca gttcccggat tccgtcgcac ggcagaatct
    13861 tgaggcatgg ataaagaagt acgggaacaa gaagtcctga cgaagctcgt aatggagaag
    13921 cgttatcggg agacggcatc cgcaggaatg ccgcctccct ttgttttggg ggtaccgcca
    13981 ccatggagaa caccagtcct cagatcgtag tcgtgggcag ttccaatacc gaccttgttg
    14041 tccgcgttcc cgaattgccc cggcccggtg agactctgct gggtcttgac ttccagattc
    14101 atgccggcgg gaaaggcgcg aatcaggcgg tagctgccag gcgccttgga gctaccgtca
    14161 cactcatcgc gaatatcggc gtagactcgt tcggggaagc atccattgaa aggttcagag
    14221 ctgaggggat acgggtggga gcaatcgcgc gtgatactga ggctgcttcc ggggtggcga
    14281 tgatatgggt gagtgagacg ggccagaact ccatcgttgt ggctcttggt tccaacgcac
    14341 gcctgacccc gcagcacgtg gaagatgcgt ccggtgtctt ccagaatgcc gacgtcttgc
    14401 tcacgcaact ggagacgcct ctgccgacgg tcgcattcgc cttgcggact gcccgttcaa
    14461 taggcgtcag gacgatcctt aatcccgcac ccgccacggt cctgggtccg gaattgcttg
    14521 cggacgtgga ttggattacc cccaatgaga ccgaagcagc gcggatttcg ggaatcgaag
    14581 tgcaaaatgt ttcagacgct gaacacgcgg cgaacgcttt gcgcaggatg ggagcccgga
    14641 acgtggtggt tacgatggga gaggcgggag ccctcctgat gaccgacgat gagtcgctgc
    14701 acgtgcctgc ccgccgcgtt ccggccgtcg acacggtcgc cgcgggagat gcgttcaacg
    14761 gcgcgtttgc ggttgcactt gccagaaacg cacccccgag ggaagccgtt gaattcgcct
    14821 gcgccgccgc gtcgctctct gtgacacggc gcggtgcgca gccggccctg cccacggccg
    14881 acgaagtcac caagttcgcc agtcagttgg cctgatccct acgctgcggc tcgatcattt
    14941 cccccattcc agatcaattg cctgccgggc aagctcgacc caacgcgctg cccgcattgg
    15001 atctccagcg agagtgtgta cgtccttcat gacaatttca acactgcact tgcgggcgca
    15061 gcgaagggtt tttgccacgt cgttcctaat cgcattttcg tcgaacacgt ccgtgctgat
    15121 aagcgtggga ttcggtttgc ggctatacac gtactttccc gcaagcgctt ctcccatcat
    15181 ttcctcgtca caccacgggg aaacggacac gctccgcaga ttgctgaact tcttgatcac
    15241 atgccagcgg ctgtgaaccg gttcacagca tccataatag cagagaccga atcgttccac
    15301 gacgtctttt tgatacggga agatgaactc ttcaaattgg ccggggccga cgccgaccgt
    15361 ttcctgagac tcggaaagca cccagcaatc cctggtccgt actttgccgc ccggcttcca
    15421 gtctttttga ggaagttcgc gcgtgtatcc tctgcttccg gagccgatgt aatcgttttc
    15481 gttattgagt gacagcagcc cctctttttc cagccattcc gcgaacgcga tgtgatcatc
    15541 ccgcagaaac cccatgagcc ggtgcagccc tgccgggttg tcgtacatcg ccagcatgag
    15601 gttctccagg ccgatgagat cgatggcaat gattgtcatt ccggtagtcc accagtaccc
    15661 accccgctgc ctcacaccga ggatgtcacc gaacacgtcc tccaggcgat ccctttcgag
    15721 aagcgacgat tcgcgatcca cggagaaaac gcgcggtttg agcttgccga agtccgcgtc
    15781 caagtccttg accggtgctt cccaggagta actcccgacg accttaccgt cgacccgttg
    15841 aattttgtcg ctgtcgtgac gcgttgaggt tacaccgtaa ttacctgcgg aaacatgcca
    15901 attccaggtg aggtatggtt caataacgtc atcgtcgcga atgttccgga agtgatagat
    15961 cgttgttcga aggtgcttct cttgtttccg tgcccagtca tcgcggcaac tcaatatgtc
    16021 gtccgttacc agttcatccg ttgccggaaa cgattctatc aacaccagcg gccgtcgagg
    16081 tttcaggtcg ttgtgagcgt accacgcgtc tttcttttcg aggttttcat cgctgtgcgc
    16141 gatatcacgg atttcctttg cgagatcgcg gagcacctgc cgatcgtgcg tcgtaatctg
    16201 cggtgaggat acgcctgtca tctcggcctc ttcgtagtgt gcggcggttg gtctgaatta
    16261 tttggaaata cgaaaactgc gcacaatctc cgcgacgcgc cgtacgctct caatatcccc
    16321 tgaggggggc aattcgtcag aaataccgag tatgagactc ggagcgaacg tctgaagaac
    16381 aagcgtcgcg aattcctcaa cgtcatcccg ggtgaaaggc gggatgaaat gtgttgcggg
    16441 gatcccgtca ataagaacgt agtcgtgccc catcgcttcc gcgatctcat caagcgacac
    16501 gtcacccatc ggggtggggg tgaacgcttc gtacccgtca aatccgcact ctcgcacaaa
    16561 aggaagcaga cctttgagcg caccgtccca gtgagggtac gaggccttcc ccgccgcacg
    16621 gagttccgcg gttcgacgcc tgtaataggg aagcgcgtat ttcctcatga gtgacggcgg
    16681 cagcatgttc gaatccacgt tgtctccgaa attgagcaac ggcagagggc ttttcttgat
    16741 cacgtctatg tacttctggt cgtcttcttc aaggaaacga atgaacgagt ccatttcctc
    16801 cgggaactca tggagggcaa cgatggtgtt ctcaaatccc atgaattcga tgaacagcct
    16861 catgagggga gtacggaggt tgtacaccga accgactcct ctgtccccga ccagtttctc
    16921 ggcttcccag aaccgttctt cactgaactc gaaattcagc gcttccagcg tataccgaac
    16981 aatccggagg tcagcaatcg atttcaccgg aaactcggta agaaggctcg attccgccgt
    17041 cttcccttcc acctcggtga gcactccaag cggggtttcc cacagacgga cgcgacggtg
    17101 tggagtgtct tcgacaaccc tgaaggacac cgaggaatcg tacacacggc tgatacacgg
    17161 gttgtagaac tcgtatcccc ggatggagca atcgaggtcg acgtacacct cgtacagaga
    17221 catgtcccgg tatcgttctg gcagcgttcc tgcgcgctta ttcgtatagt accagtggtc
    17281 tatgcgcggt tgccacacta ctccgtccac cggttggtga tgcatgattg cgagaactcg
    17341 ttctctatct gtcacgttcc cctccaaaat ccatcggcgc ggaaaccttc cgtacgtact
    17401 ctaacgaaaa tggcgtttgg tccaagggtt cgctcttttc gaacgcttcg ctatatttga
    17461 tggagccggt tctcaatcac actcacacgc ggtacccatg cactctcccg aacatcccca
    17521 caaaagtgac tctcagatcc cgagcactgc cgtcaaaggt aggatcttcg acgtacagac
    17581 gttttcgatc cacgacggtc cgggaatccg cacgaccgtg tttctgaaag gctgccccct
    17641 tcgatgcgta tggtgccata accccgaatc catggaaacc gaacccgacg tgggtttcat
    17701 tccggcgaaa tgcatcgcat gcggtcagtg cacgctcgtg tgcacgcatg agggccacga
    17761 gatcgtcgac ggcaggcacg tgttccagcg gggtctctgc gtacggtgcg gctcgtgcac
    17821 gacggaatgc ccaaccggtg cacttgaact tgcgggcaaa gaggtcactg ccgcgtgggt
    17881 gatgtccgaa gtggagaagg accgcatgtt ctacgagcgc tccggcggcg ggataaccct
    17941 gtcgggcggc gagccgctca tgcaaccgga gttttccgcc gcgctgcttc gcctggctcg
    18001 cgattcccgc ctccacacgg cagtggatac gtccggttat gcgggcgaaa cggtgttcga
    18061 tcgagtgaca gccctcgcgg atctggtcct tttcgacata aaaatgatag acgaacgcgc
    18121 acaccggcga ctcaccggcg tgtcgaaccg gcgcattctg cgaaatctgc aacgccttct
    18181 tcgggacgaa aacggcccca aagtatggct gcggctgcct gtcatccccg ggcagaacga
    18241 ctcgcccgac gccatccaga acctgatcga tttctgtgcg tcgctctggg gtaatccccg
    18301 ccttgaagca attcacctga tgccctacca cagacttgcg gaaagcaaat accaggaatt
    18361 cggaaaacgg tatcttatta atggaacgca cccgcctgct gatgaagaca tttcggcaat
    18421 catacgccgt ttctccgaac gcggagtgcc ggtaacgcgc agttagcgcc ttccacaacg
    18481 gagatgcatg ctcctatgcc caccctcggc cgtgacgaag aagtaattca gggcgcgtac
    18541 ggaattgacc tttcaccgcg cgtggaacgg ttgcgcgatt acgttctcaa cgacgcgaca
    18601 tgggctgacc attacgcccc gcgggcgtgg ctggttgccg aaagcatgaa gcagaccgag
    18661 gggcaaccgc cgctgttccg ccaggcggcg gcactggcac atgttatcag gaactacccc
    18721 gtaacgatcc gcgaaggcga attgatcctg gggtatcagc agcacgaatc cttccgggga
    18781 atcgggtggc tggaatttga taatctcacc aacggcgaac gcagagaagc gctccgtaat
    18841 cacgtccaga atctgagtat cccggacgag tggaaacaaa aggttttcga cgcaatcgac
    18901 tactggcgcg aacaccctcg cgtaatccct catttctggc cacaaacacc cgattacata
    18961 tcccgtgaca cgcgggcagg cgtctatttc ggcggcggtg ggagcgaagg gcataccgtt
    19021 ccgggatacg aacgggtttt gcggcacggc ttccatgcga tccttgaaca gatagacggc
    19081 gagatcgcca gactccaacc gtggaagccg gacgatcaga aaagaaaact gtggctccac
    19141 agcgtgaggc ttatcgcggc agcggcgtgc gaacttggaa agcggtatgc cgaggaactg
    19201 cgagccctgg ccacgcgcga aagcgaccct cttcaggcgg aagagtaccg tcgtcttgcc
    19261 gacatctgtg agagagtacc cgcccgcggc gcgcggacct ttcacgaagc agttcaatca
    19321 ctctggttct gccatctgat catgggatgg gaagacggga ttaacgcaaa ctctttcggc
    19381 cgttacgacc agtacctcta tccgttctac gccgccgaca gggaagaggg gcgaatcaca
    19441 gaagacgaag cgctggaact gctcgaagcc ttccatcttg cactctacaa gagctacgac
    19501 gtccagcagg ccaccatcgg cggccagaac ccggatggaa cggacgcaac gaacgaactc
    19561 acttacctgt tccttgaagc gatgtggcgg gtgaatctga ttcgctgcct atctgtgaga
    19621 gtccaccggg gcaccccgcg aaggctcctc gaatccgcat tccggttgat caggcggggc
    19681 ggaggcgtgc cgttcttctt caacgacgaa gcgattgttc cggcccttgt ggagaagggg
    19741 attcctgttg aggatgcccg ccagtacgcc atcataggct gcgtggaggt gacgattccg
    19801 gggaaaacga acccccacgc ggtaagccac atgatgaacg tggccaagtg cctggagctg
    19861 gcgctgaacg acggcaagga cctcgcaacc ggcgaacaga tcggaccgca gacggggact
    19921 cttgcggaca tgcctgacat cgaagcgctc tggaacgctt acaaacttca gctcgagcat
    19981 ttcgcccggc acgcggtgga gctttcgaac cagggcgacc tggcgcaacc tttccgcttt
    20041 ccgctgccct acacgtccat cctgaccgac gactgcatag cgcgcgggaa ggatatgacc
    20101 gacggcgggg cgcattacca ttaccattct acctgcgcga taggcttgcc caacgtcggt
    20161 gactccctgt acgcggtgga ccagctcgtc ttcaaggaga aaagcgtctc ccccgacgaa
    20221 ttgctggaag cgctcaaggc gaactgggaa ggggaggaac gggagaaact ccggcagaag
    20281 gtgatccaca ggctcgcgaa gtacggaaac gggaacggcg acgtggaccg gtgggtggaa
    20341 cgtgcggccc accactactg cgatcacatg gccacgtaca ccacgccgca tggtgggtcg
    20401 ttccatgcgc acctgttctc attcgtatgg catctcgact gcgggcgcgc caccaatgcc
    20461 accccggacg ggcgcaaggc gcacgatccg ctggcgtaca gcgtctcccc gcaacagggg
    20521 cgcgacgtcg aggggctgac ggcgctgatg cggtcgttga tgacgttgcc ccacgaacac
    20581 gctgcgggtt cgtcatccgc gatcatcgag atcgccccga aactgctgga cgaagtgtcg
    20641 ctggacaagg tccttgatgt cgttcaaacg gcgattgaca aaggcgtggg gcagatgcag
    20701 ttcaacgtga tcgacagcgc caccctgcgc cgcgcacagg agaaccccga ggcctactcg
    20761 catatcgcgg tccgggtgtc gggtttcagc atgcgcttct gcctgctgga caagacgatg
    20821 caggaccaca tcatcgcacg cacaaaacac gaagagatgt gaacggacgt ctccggagga
    20881 attggagtga tacagccgat tctgcgtaca gaacgcatcg cgcggctccg aaagtggcag
    20941 ttcgatcagc gcggggggca ggtggatcgc gagatgctct ggcaccgcta catggccggg
    21001 catcagagcc tcccgataga cgcacggtat gccggcgtgt ttgcgtacct catggataac
    21061 ctccccgtcg agatcaacga cgacgaactg atcgtcggcc ggatcaaacg gcgcgagatg
    21121 acgcccgagg aaaaagcgga acgtgaacgt atcgcaggta gtacctttgg tgatccattg
    21181 ggcgacatat tgaagaaact catttcgccc gatgcgcatg agtcggtcgc cgaaggccgc
    21241 catgtggcag gcgcgatgac cggtcacatg acgcccgatt acccggctgt gctcaggaag
    21301 ggcctcaacg gaatccgacg cgaggttcgc gaacggctga cacgcgcgac aactgagaag
    21361 gaacgagcgt tttgccacgc cgcgctggag gtgttgtccg gcgcaatgcg gatggcccgg
    21421 cgctacgcca cgcacgcccg cgaactggcg gccaccgcaa cccccgaacg gcgcgaggaa
    21481 ctgctccgca t
//
LOCUS       DAOSOO010000030        21403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2465_length_21403_cov_5.771548, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000030 DAOSOO010000000
VERSION     DAOSOO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 21403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21403
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2465_length_21403_cov_5.771548"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..814
                     /locus_tag="PLT86_03765"
     CDS             5..814
                     /locus_tag="PLT86_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT35766.1"
                     /translation="MNGRPSYASAGSRTSLTIKGSDTMVHLVSTWAEAFMRTNPDVEI
                     SVTGGGSGTGIAALINGTTDICAASRNIKDSERARAQQNGRSAFETVVARDGIAIVVH
                     PSNTVNTLSHDQLKKIYTAVYTQWNQVGGPNRPILALSRESSSGTYVFFQEHILEQQD
                     YAQSVRLMPATSAIIQSVSTDRGAIGYVGIGYAHDAGRRVKVLKIKATESSPAVTPSE
                     ATVRSGEYSIARPLFFYTNGAPTGAAKRFVDFCLSSEGQRIVRETGYVPAR"
     gene            1040..1903
                     /locus_tag="PLT86_03770"
     CDS             1040..1903
                     /locus_tag="PLT86_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT35767.1"
                     /translation="MRNVTTRTLTAISIGLLGMLAACSGKKGNEVTVVTVKGSDTMVH
                     LVSMWAEAFMKNHRDVEISTTGGGSGTGIAALINGTTDICASSRDIKDTERAQAEQKG
                     ITPVETMVARDGIAIVVNPKNPVQELSLDQLRKMYTGVYTRWSQVGGPNEPITALSRE
                     SSSGTYVFFQEHVLNKEDFAQTVRLMPATSAIIQSVVLDRWSIGYVGIGYAHEAGPQV
                     KILGVKATEAGAAVMPTEAAVHSGEYSIARPLFFYTNGDPTGAVKQFVDFCLGPDGQR
                     IVRETGYVPVQ"
     gene            1941..2825
                     /gene="pstC"
                     /locus_tag="PLT86_03775"
     CDS             1941..2825
                     /gene="pstC"
                     /locus_tag="PLT86_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT35768.1"
                     /translation="MPRPRTKWTERCVEVTLVVAASTSIILVGLIFLFLFREAAPFVQ
                     HPGLSSLMGDRWIPVSFQKEQFGIYPLVTGSILVTAIATVVAVPFGVIGAVYLSEVAR
                     PAETETLKPFIELLAGIPSVVIGFFGLVVLAPFVKAAFGLETGLNAFTGGLLLGLMAI
                     PTIMSVSEDALRSVPASYKQASLALGGNQIQTIWRITVPAALPGIVAAVMLGMGRVIG
                     ETMAVMMVTGNAAQTTFKPFESVRTMTATIAAEMGEVPFGSDHYRALFWVGIILLVAT
                     FLLNIVAQRVLKRYSVRQ"
     gene            2822..3673
                     /gene="pstA"
                     /locus_tag="PLT86_03780"
     CDS             2822..3673
                     /gene="pstA"
                     /locus_tag="PLT86_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT35769.1"
                     /translation="MRQRSNGFLAIWAMRIIFYLLVSSVGIIVLDILWKGLPSISLSF
                     LLDSPRRSGAEGGIFPAIVGTVYLVTGAIAAALPLGIASAVYLSEYAKQGLFTRGVRF
                     AIITLAGVPSVVFGLFGLGLFVIFLGFGASVLAGSLTLACMILPTIIVASEEALQAVP
                     RELREASLALGATRWQTIWRSVLPYSVSGIMTGSILGIGRAAGETAPILLTAAAFFLP
                     NLPESPFDQVMALPYHLYILATQHPEAAKVRGMQYGTALVLLVLVLGINMAAILLRGR
                     FRKKYRW"
     gene            3667..4446
                     /gene="pstB"
                     /locus_tag="PLT86_03785"
     CDS             3667..4446
                     /gene="pstB"
                     /locus_tag="PLT86_03785"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT35770.1"
                     /translation="MVETGERTSDAKIVVEHLDFHYGSVQALFDVSVEMAPRKVTAFI
                     GPSGCGKSTFLRTLNRMNDIIPGTRVQGRVLIDGHDIYSNGTDVVALRRSVGMVFQKS
                     NPFPKSIFENVVYGPRIHGEKNSARLREIAEQSLQRAGLWAEVKDRLDQNALSLSGGQ
                     QQRLCIARALAVGPEILLMDEPASALDPRSTARIEDLIGELREDYTIVIVTHNMQQAA
                     RVSDFTAFFYEGHLVEFGETQQIFTMPKKKQTEDYITGRFG"
     gene            4484..5161
                     /gene="phoU"
                     /locus_tag="PLT86_03790"
     CDS             4484..5161
                     /gene="phoU"
                     /locus_tag="PLT86_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT35771.1"
                     /translation="MSGHLQREIDNLKKKLLALGAFVEEDVQKAVTAISERNASAARE
                     VIESDPDIDKMEIDIEEDCLKVLALHQPVAIDLRFIVAVLKINNDLERIGDLAVNIAE
                     RAAFLATQERVDIPFDFVGMASKAKTMLARSLDALINGDTELAHSVCAADDEVDQINR
                     EMYYQIQDGIRNSPEQMVCLLHLLSVSRHLERIADHATNIAEDVIYMIAGEIVRHRVE
                     EFTQKRS"
     gene            complement(5264..5641)
                     /locus_tag="PLT86_03795"
     CDS             complement(5264..5641)
                     /locus_tag="PLT86_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOT35772.1"
                     /translation="MRELVETSKAPRPIAPYCQAVKANGFVFVSGQGPLDPATGQIVQ
                     GTIEEQTRLTIETIKAILEAAGTSLENVVKVGVFLQDMKDFRAMNRIYGEYFGEIKPA
                     RTTVHAEAPVDIKIEMDCIAVMP"
     assembly_gap    5997..6096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6097..6672
                     /locus_tag="PLT86_03800"
     CDS             <6097..6672
                     /locus_tag="PLT86_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35773.1"
                     /translation="GRFGTVLEARATIFKALPLADDAWRLIPEQSGGGPLMDLGAHTI
                     DILTFLLGPMESAVGFAANRATNWRVEDTVSLLLRARSGAIAVVEHSFCAKGGDQTLE
                     VNGTEASALVSTPPPGGEKPFLRVAGPQGVVNEPVPFENYYQLQIEHFAECLSGNVAP
                     LATGEDGIHNLAVIEAAYRSVQTNRVEAVQT"
     gene            6669..7499
                     /locus_tag="PLT86_03805"
     CDS             6669..7499
                     /locus_tag="PLT86_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT35774.1"
                     /translation="MKRYALRGVLAVVLVTMVGCAPKRVERPRGPQQPPMVRIPAGEY
                     IIGTDDGPENERPAHKVRVRAFMIDKFEVTNRAYLQFVRETGFPAPPRWLPDSTILGS
                     TDRLPFDEREADLPVAYVSWQDADAYARWRGCRLPTEAEWEIAARCSTAMLYPWGNDS
                     LATDGKHAANTGGESDGFPGLAPAGSFGNGTSCWGAEDLVGNVWEWTADWYIPAAYRE
                     ASPTGFAEVVSDSLFRQRVIRGGSWFDPPGRVKATTRTGFDPSFRSDIIGFRCARDSE
                     "
     gene            7512..8273
                     /gene="tatC"
                     /locus_tag="PLT86_03810"
     CDS             7512..8273
                     /gene="tatC"
                     /locus_tag="PLT86_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT35775.1"
                     /translation="MKDDAPLLSFWDHLEELRWRIVKSILALLIGGIVSLFFSDRILA
                     LLLLPLDKLQGTVRLVNLSPLSMVMVRLYIAIIAGLLVGLPVIVYQAWSFVAPGLYPH
                     ERRAVPWVLISTFLLFVIGASLSYTIMPFLFRVLVEAGYQGIENFWNIREYMGFLLGF
                     MGAFGVVFEMPVAVYILSVVGIATPAGLRRSRKYAIVLIFVVSAVLTPSPDPFSQIAM
                     AVPLVALFEVSIGVSALVHRRKKRRREAAESENAQ"
     gene            8391..9149
                     /locus_tag="PLT86_03815"
     CDS             8391..9149
                     /locus_tag="PLT86_03815"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOT35776.1"
                     /translation="MLPVRLGVNLDHVATVRQARKTTEPSPVQAALLAELGGADQITI
                     HLREDRRHIQDDDLELMRRVTQLPINQEMGVTEEMLGIALRIHPNVACLVPEKRVEVT
                     TEGGLDVVGNRERVARAMERLHAAGIAVSLFIDPDPAQVRIAAELGAEAIELHTGEYA
                     NSRDAEQATQLHRLEAAAVLGCDLGLEVHAGHGLTYQNVSRVASIPQIVELNIGHSII
                     ARALYVGLRQAVAEMRTLLAEARNEARLNSLSDK"
     gene            9163..11904
                     /gene="secD"
                     /locus_tag="PLT86_03820"
     CDS             9163..11904
                     /gene="secD"
                     /locus_tag="PLT86_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT35777.1"
                     /translation="MPRGLQIRIALLLVIIGWAVYQLYPTFVFWPLTVQQRQALQLTD
                     KAKFDDLQMRAIKRGLDLQGGVFLVLEVDPARTMTGGTLADAVEGARRVIEQRVNQYG
                     VSEANVQKIGERRIIVELPGLDNIEAAMRLVGRTALLKFNLLRPVSERDEVVKRIDAV
                     LPQLLSGVVIDTTASVDSSAAAAATPASRAATDSTPASQGELFGGARQPQTAQTGVAR
                     ADSARENPLKGLTSFSGLLNVGPGNDYYVSTVNKPKVEAVLSLIAEKNPGLIPSAGEF
                     LFGKEELINGQYVTPLYYVSSNAEMTGASVKDAQVSQGSNLDIEQAGRPVINFEVKED
                     SVRLFSALTRRNRGERMAIILDNVVYSAPTIQAHITDGRSIITGMADKEEARSLAIAI
                     RSGALPAEVQVQEQRYVGPSLGTDSIESSLRASMIAGIAVIAFMVLYYRLGGAMAVVS
                     QTLNLCLVMASLAALHGTLTLPGVAGLILTLGMAVDSNVLVFERMREESNSGKSPRAT
                     IESGFKNASSAIWDSNITTLISAIVLYQFGTGPVRGFALVLAIGIGGTVFAALFITRL
                     FYDLYAYFGRLRTISLGIPFLKNPHFQFLKWRKPWFIGSGIVLVVSVASILTVGFNWG
                     VDFTGGTRLAVRFVPPVKPGDVRTALRRVPVGNRVLDLSQSEIKTVDEATDLLITVKQ
                     ISNIEAAAVDSALRIRLSTAFPANTKGDWVLNAESVGPKVGSELKSSAIYAVLFSLIG
                     ILIYLSFRFEWIYAVTTTASLFHDVLLALGIFALIRHEVTLSVVAAVLTMVGYSLNDT
                     IVVFDRIREGLRIHGRRLTFGEILDKSINETLTRTIITSGTTLLAVVALLIFAGQTLK
                     DFALVLFIGVLTGTYSSIYIAGALVFEWQNRQQSRAAETARKRGASAVNKTKKSATA"
     gene            12121..12399
                     /locus_tag="PLT86_03825"
     CDS             12121..12399
                     /locus_tag="PLT86_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT35778.1"
                     /translation="MPTYEYQCESCGHRFERFQSIKADPLSECEICKGKVRRLISAGG
                     GLIFKGSGFYITDYRSEGYKAQSKRDNGGDSSTSSTVTSKETTAKTTT"
     gene            12470..13009
                     /locus_tag="PLT86_03830"
     CDS             12470..13009
                     /locus_tag="PLT86_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35779.1"
                     /translation="MNRVSVLATCALFAATMMACGRSPEALLKKGNAALLETTKPQPD
                     TATIDDGVKALREFTTRHMGHEHADSAYYLLGMIYGATQRNAEAGETFMTLVQNFPTS
                     RLRPKSLILASQAYENVKKYGHAKQCMQTLMAEYPDHEFVKGGSAKWLFLNIGKEPEA
                     WDQSFISDSSTARASTRPR"
     gene            12949..13935
                     /locus_tag="PLT86_03835"
     CDS             12949..13935
                     /locus_tag="PLT86_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOT35780.1"
                     /translation="MGSELHLGLINGTRVDPAQVSYTGVVPHRGILHVPLLSLCVVLL
                     LAVGDAWPADDIRIEAQLDRGTVALDETATLVVKAEWEGPSDRFALVIPQAPRVHNLI
                     VIGSKRGTRSWADEKGEHSAQEFSYVLQPESEGNAAVGAIRVQFTTPEGASGDLVTQP
                     LQLRVGSPVHTRWCFSWGDVGGLVVLAGLAGYAGWRRWKKKTVRETASLRGQKDPFAA
                     ALSEIASARKAGNQKAFYGAVLSLLRQLTSERTGVPTAALGARDLVPFADRLVSDESS
                     AERLRDLLSRIDTILYAPATDDLDADMDVTELFVRNLVSENQIGKRERKDAS"
     gene            13925..14920
                     /locus_tag="PLT86_03840"
     CDS             13925..14920
                     /locus_tag="PLT86_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT35781.1"
                     /translation="MPLDSHIQEIHAEIERESEFVERIVSEIGKVIVGQRTMVERMLI
                     GLLCDGHILLEGVPGLAKTLAVSSLARTIHAKFQRIQFTPDLLPADVVGTPVYEQKKS
                     TFTVKKGPIFANIVLADEVNRAPAKVQSAMLEAMQEHQVTIGDQTFALEDPFLVLATQ
                     NPIEQEGTYPLPEAQIDRFMLKVVVTYPSKAEERQILDRMTEGDVPHVNEVVEPADII
                     RARQLVRRIYMDEKIKNYIVEIVCATRKPAEYGLADYTDFVDYGASPRATIWLALASK
                     AHAFLRHRGYVTPEDVKAIGMDVLRHRVIPTYEAEAEEVTAEAIVSKVFDSVEVP"
     gene            14917..16014
                     /locus_tag="PLT86_03845"
     CDS             14917..16014
                     /locus_tag="PLT86_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT35782.1"
                     /translation="MIQKEILKKVRRIEIQTRQVVNTLLSGEYHSALKGQGMEFAEVR
                     EYQPGDDVRRIDWNVTARMRRPFVKIFDEERELTVMLMVDASSSGDFGSVEQTKNQIA
                     VELCALLAFSATKNNDRVGLIVFTDRVEKFVPPKKGRKHVLRVIRELLAQFSRTETSE
                     PRPFRSRPHPVMGGLASLAIVAAAASMISAIGIPETTYQWLAETVISILGAAGVGVVS
                     WWAFRTYGADKGRGTDIAGALDFLNRVMTRRGVVFLISDFQAAGFEKALKAARRKQDV
                     IAITLTDPREVTLKGAGLLALEDPETGESVVVDSSGKRFRDWYEQQARTRHAQRKRLF
                     RTTGVDCVDVWTDTSYVDPLVRFFSARAKRM"
     gene            16123..17028
                     /locus_tag="PLT86_03850"
     CDS             16123..17028
                     /locus_tag="PLT86_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35783.1"
                     /translation="MRYIRGLLVIGLVAFGTGCSKSGVDPEKQRVFATELLNRELYAE
                     AIRAFDKYLEMPAVSDRDRVDAMRKLADALFDRANDYHSALVYYLRLRVFAPDYPEMN
                     EVRARLVTCFERTGRSADASLMRREIAQGKTLPPDSLAGPVVAEFGGRKISEREVLRE
                     LEQLPPELRQQFSTMERKRELLRQVVGREILYEMAVKRGYADSPEFQLQLDRIRRDML
                     VQRIGEEQLGSLPDITEADVRRFYEEHQAELPRVPGGGVPSLQQIRPQIEMAARQAKQ
                     QEAFQRLVDQLFASQEVKLYPERMR"
     gene            17123..17773
                     /locus_tag="PLT86_03855"
                     /pseudo
     CDS             17123..17773
                     /locus_tag="PLT86_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            17854..18663
                     /locus_tag="PLT86_03860"
     CDS             17854..18663
                     /locus_tag="PLT86_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT35784.1"
                     /translation="MKGTWTVFVKELRSYVHSPVAYVMAFVFLILCGYFFQDAMNFLN
                     RYTTDYPMRYQQAQQMRRDPPPPPNVDIVVIRRTYSVMAFLLTFIMPILTMRLYSEEY
                     RNGTIELLFTSPVTPGQTLAGKFLASFALYAGILVLTLNFVVFAYLYAEEGVRPDTGL
                     ILSCYLGLLFLGASFISVGVFASALTENQIVAAVVSFSVLLMFLVIGAAADYVGNYSA
                     AEFLRYLAVNGHADSFLRGVISLRDIVYYITFIGFMLLLTNRVLESRRWRL"
     gene            18664..20424
                     /locus_tag="PLT86_03865"
     CDS             18664..20424
                     /locus_tag="PLT86_03865"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT35785.1"
                     /translation="MVRSIPIPFIALVGLLLAVDGMVAWLASATVWGWGIPWAVGTGL
                     LLGAFLSRLGIRKSVAFAAALVVLIALCAIIYVKGEVIGAFKLDSITAILLFLGLFVG
                     EVGVGIASVLTGRASAQGANFWVYVVSALAIAVVLNYLTNRHVRTTYDITQAQLESLS
                     DQTLAKMKGLRKDVHLIAFIRDSDPNRPGYAEMLKKYAAASRYFTFEFVDPDKYPDIA
                     ARENINPNDITIIVKCEDRRELVLGPEERDLTTAIIKVTRPGQKRIYFSMWHQERTLE
                     GEMGMLANLLRQLNYATAPLKLIDSDVPPDCAVLVIPGPKAPFAPVEISRIERYLAEG
                     GSALVLLDPDSIETGLENVVARWGVVVHPNMVIERQRGLVPYAGGLYMGEQQSMYARA
                     MKYEPSHAIVADLESKGIATGFYQVREITWTGANPTLACTGTPIVFAGSRTSFAITEV
                     KEALRNPRQAHSAEGKPSGPFSVAVAATAPPSVSTGVEGAKTRLVVVGDADFATDRVV
                     SQERGNVDFMLNCITWLSEDEDLITIHSREPGYKPIVMTASQGTFMFLFCVWRYPAMV
                     FVAGLLIWWYRRSRGPGKDN"
     gene            20539..>21403
                     /locus_tag="PLT86_03870"
     CDS             20539..>21403
                     /locus_tag="PLT86_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT35786.1"
                     /translation="MKKWIRTIILVVIALALGFWAYFGERHLDKIALEKRLEQKITHI
                     PTEKIAGFTLIINNPNAPKPDTTVVERVGSDEWQIVHPILWPGESTEWRALAQNLPRA
                     WKQRAWKSSPDSVAFFGLKPPLVRLIIKPVDSAMRPDTLDIGKATPTDNFCYVKYPRS
                     DSIYTTDVILVSSTLKSLHTLRDKSVTPIEPSLVQRVTVKRGGRDSMVIERRGNLWWM
                     TSPLERSVDRDTVNALLKEVQMSRVAVFHDTPAAMGTYGLDRPVAEVEFLMAYQGRLA
                     RRTLLLGSKVPN"
BASE COUNT         4516 a         5717 c         6487 g         4583 t
ORIGIN      
        1 ggcactgaac ggaagaccct catacgcgtc cgccggatcg agaacgtcgt tgacgatcaa
       61 aggttcggat acgatggtcc atctggtaag tacctgggcg gaggcgttca tgcggacgaa
      121 tccggacgtg gagatatctg tcaccggcgg agggtccggc accgggatag ccgcgctgat
      181 caacgggacg acggacatct gtgccgcaag caggaacatc aaggattccg agcgcgcacg
      241 ggcacagcaa aatggacgat ccgcatttga aacggtggtt gcacgcgatg gtatcgcgat
      301 cgtggtgcac ccctccaaca ccgtgaacac gctttcgcat gatcaattga agaagattta
      361 caccgcggtg tacacgcaat ggaatcaggt tgggggaccg aacagaccaa tactggccct
      421 ctcgcgcgaa tcgagctcag gtacctacgt ttttttccag gagcacatcc tcgaacagca
      481 agattacgcg cagtcggttc ggctgatgcc cgcaacttcc gccattattc aatccgtttc
      541 gactgatcgt ggcgcaatag ggtacgtagg catcgggtac gcgcatgacg cgggccggcg
      601 cgtcaaagtg ttgaaaatca aggctaccga atccagccct gcggtgacgc ccagcgaagc
      661 gaccgtaagg tcgggggagt attccatcgc gcggcccctc ttcttctaca cgaacggggc
      721 tccgaccgga gccgccaaac ggttcgtgga tttctgcctg agctcggaag gccagaggat
      781 agtccgggaa acggggtacg ttcccgcccg gtaggcgttt tgctcgtcgt gcatttccat
      841 caaagaacga attgcacgga tccgggcaac gccaggaatg gaggtgggtt caccgcgacc
      901 cacctccgcc ctttgctccg gtgcccacag caccatccat tacaatcccg ctaacaaatc
      961 cttaacccgg ttcaaacgga atcctcaccg gagcggccga ccttcattgt gaacacaact
     1021 gccgaaatgg aggtgaaaca tgagaaacgt taccacacga accctcaccg caatctcgat
     1081 tggtctcctc ggaatgctgg cagcatgttc ggggaagaaa ggaaacgagg tgaccgtcgt
     1141 gacggtgaag gggtcggaca caatggtcca tttggtaagc atgtgggcgg aggcgttcat
     1201 gaagaatcac cgggatgtgg agatatccac taccggtggc ggttcgggca cggggatagc
     1261 cgcgctcatt aacgggacca cggacatctg cgcctcaagc cgggatatca aggacacgga
     1321 aagagcgcag gcagagcaga aggggatcac tccggtggaa accatggtgg cccgcgatgg
     1381 aatcgcgatc gtggtgaatc ccaagaatcc ggttcaagag ctttcccttg accaactccg
     1441 caagatgtac acgggggtgt acacaaggtg gagtcaggtt ggcggcccga acgagccgat
     1501 tactgcactc tcccgtgaat ccagttccgg cacgtacgtg ttttttcagg aacatgtcct
     1561 gaacaaggaa gatttcgcgc aaacggtgcg gttgatgccg gcaacgtcag cgatcatcca
     1621 gtctgtagtt cttgaccgct ggtctatcgg gtatgtcggc atcgggtatg cccatgaagc
     1681 gggtccacag gtgaagatcc tcggggtgaa ggcgaccgag gccggtgcgg cggtgatgcc
     1741 gactgaggcg gctgtgcact cgggtgagta ctccatcgcg cgtccgctgt tcttctacac
     1801 aaatggtgac cccacgggag cggtcaagca gttcgttgat ttctgcctgg gacctgatgg
     1861 acaaagaatc gtcagggaga cgggatacgt tcccgtccag tgacatcgaa accaaccggt
     1921 tcatcgctca ggaaatcgtg atgccacgcc cgcggacaaa atggacggag cgctgtgtcg
     1981 aggtgacact cgtggtcgcc gcttccacaa gcatcattct cgtggggctg atcttcctct
     2041 ttcttttccg ggaagccgca ccatttgtgc aacatccggg tctttcctcg ctcatgggtg
     2101 atcgctggat accggtgtcg ttccagaaag agcagttcgg catctatccg ttggtcaccg
     2161 gttccatact ggtaaccgca atcgcgacgg ttgtggcagt tcctttcggc gtcatcggcg
     2221 cagtgtatct ctccgaagtg gccagaccgg cagaaacgga aacgctcaaa cccttcatcg
     2281 aattgctcgc aggaattccg tccgtggtga tcggtttctt cggtctcgtg gtgctggctc
     2341 cgttcgtaaa ggcagcattt ggtctggaaa cgggcctgaa cgcgttcacc ggagggcttc
     2401 tgctcgggct catggcaatt cctacgatca tgtcggtttc tgaagacgcg ttgcgcagtg
     2461 tcccggcctc gtacaaacag gcctcgctgg cgttaggggg gaaccagatt cagacgatct
     2521 ggcgaatcac cgttcccgcc gcgctgccgg gaatcgtcgc cgcagtgatg ctgggaatgg
     2581 gccgtgtcat tggcgagaca atggctgtga tgatggttac cggaaacgcc gcgcagacca
     2641 cattcaaacc gttcgaatcg gtacgcacga tgactgcaac gattgccgca gaaatgggcg
     2701 aggtcccgtt cggaagcgat cattaccgcg cgttgttctg ggtcgggata atccttcttg
     2761 tggccacgtt cctgttgaac atcgttgctc aaagggtact caaacggtac agtgtgcggc
     2821 aatgagacaa cgctccaatg gctttctcgc gatctgggcg atgcggatca tcttctacct
     2881 gctcgtttcg tcagtaggca tcattgttct ggacatactc tggaagggac tcccttccat
     2941 cagtctctcg ttccttctcg attcgcccag acggagcggc gctgaaggcg ggatctttcc
     3001 ggccatagtt gggacggtat atctcgtcac cggcgccatt gccgcggctc tgccgcttgg
     3061 aatcgccagt gcggtctacc tttccgaata tgcgaaacaa gggctgttca cacggggggt
     3121 acggttcgcg ataatcacac ttgcgggggt cccgtccgtt gtgttcggtc tttttggtct
     3181 cggcttgttt gtaattttcc tgggattcgg agcttccgtt cttgcgggaa gcttgaccct
     3241 tgcgtgcatg attctcccga cgatcatcgt tgcgagcgaa gaagccttgc aggcagttcc
     3301 acgggaactc cgggaagcca gcctcgccct gggcgcaacg agatggcaga ctatctggag
     3361 atcggttctt ccgtattccg tatctggaat catgaccgga tcaatcctcg gtatcgggcg
     3421 ggcggcggga gaaaccgcgc cgattcttct tacagcggcg gccttcttcc tgccgaacct
     3481 tcccgagtcg ccgtttgacc aggtcatggc attgccgtac cacctctaca ttcttgcaac
     3541 tcaacacccg gaagccgcca aagttcgagg gatgcagtac ggcaccgcgc tggtgctgtt
     3601 ggtactggtt ctcggcatca acatggcggc gattcttttg agaggccgtt tcaggaagaa
     3661 gtaccgatgg tagagacggg tgaacgaacg tctgatgcga aaatcgtcgt ggaacacctc
     3721 gactttcatt acggttcggt gcaggcgctt tttgatgtca gcgtcgagat ggcaccccgg
     3781 aaggtgaccg cgttcatagg tccttccggg tgcgggaagt caacgttcct tcggacgttg
     3841 aatcgcatga acgacattat cccggggacc cgggttcagg gccgcgttct cattgacgga
     3901 cacgacattt actcgaacgg gaccgacgtg gtggcacttc gaagaagcgt cggtatggtg
     3961 ttccagaagt ccaatccctt tcccaagtcc atcttcgaaa acgtggtgta cgggccgcga
     4021 atccacggcg aaaagaacag cgcccgactt cgcgagatcg cagagcagag cctccaacgg
     4081 gcgggtctct gggcagaggt gaaggatcga ctggatcaga atgcgctttc gctctccgga
     4141 ggacagcaac aacggctgtg catcgctcgc gcacttgcgg tcggaccgga aatcctgttg
     4201 atggacgagc ccgcgtcggc gcttgatccc cgatccacgg cgagaattga ggatctgata
     4261 ggagaattgc gcgaggacta tacgatcgtg attgtcacac acaatatgca gcaggcagcg
     4321 cgcgtttcgg acttcaccgc gttcttctac gaggggcacc tcgtggagtt tggcgagacg
     4381 cagcagatat tcaccatgcc gaagaagaag cagaccgaag attatatcac aggcaggttc
     4441 ggttgaaatc gaccagagct ccagcctggg aaggagcaca gggatgtccg ggcacctcca
     4501 acgggaaata gataacctga aaaagaaact gcttgcgctc ggagcctttg tggaagagga
     4561 tgttcaaaag gccgtgacag ccatttccga gcggaacgcc tcggctgccc gggaagtgat
     4621 cgaatcggac ccggatatcg acaaaatgga gatcgatatt gaagaggatt gcctgaaggt
     4681 gctcgcgtta caccaaccgg tcgcaatcga tcttcgattc attgtcgcgg ttctcaagat
     4741 aaacaacgat ttggagcgga tcggtgatct ggcagtcaat attgcagagc gggcagcatt
     4801 tctcgccacg caggaacggg tggacatccc gttcgatttc gtaggcatgg cctcaaaagc
     4861 aaaaacgatg ctcgcgcgga gcctggatgc gctcataaat ggcgacacgg aactcgcgca
     4921 ttcggtgtgc gctgcggatg acgaagtcga tcagattaac agagagatgt attaccagat
     4981 tcaggacggt attcggaata gccctgaaca gatggtgtgc ctgcttcatc ttctgagtgt
     5041 ctcgcggcac ctcgaacgca tcgcggacca cgcgacgaac atcgcggagg acgtgattta
     5101 catgatcgca ggggagatag tccggcacag agtggaggag ttcacccaga agaggtcgtg
     5161 acctaaattt cgccgcagag tcctgcgcgg aaaggggtgg ttctgggact acccctttcc
     5221 ttttgaagaa gagcgaccaa tgccggtgtc agccggcacc tactcacggc atcactgcga
     5281 tgcaatccat ttcgatcttg atgtcaacgg gtgcttcggc gtgaacagtc gtacgtgccg
     5341 gcttgatttc tccgaaatac tccccgtaaa tgcgattcat tgcccggaaa tccttcatgt
     5401 cctgaaggaa aacacctacc ttcaccacgt tttccagact cgttcccgcc gcttcaagga
     5461 ttgccttgat ggtttcgatc gtgagccgcg tctgttcttc gatggtgccc tgaacgatct
     5521 gcccggttgc cgggtccaac ggaccctgtc cagagacgaa cacgaaaccg ttcgctttca
     5581 cggcctggca atacggtgcg atgggtcgcg gcgctttgct tgtttcgacg agttcgcgca
     5641 tgatgaacct ccgcggaaga tggctccact gatacgagag tgattgactg tgcgtagaaa
     5701 ccaatatacc gggtctgaac tgggcgccca agcgggatta acggcgcgcc ggttgtgaag
     5761 ccacacacga agttctacat tcaaagtgct tttggggtcc gaagcatttg aatcacgccc
     5821 ggagcggagg aagaacgcat gaatgttcgg tggggagtga tcggagcggg gggcgtcgcc
     5881 cgacgaagaa gtatgccggc gatccgacag gcgaagaatg ccgaacttgt cgcgctgatg
     5941 gtgcgggata gggagagagc ggacgctctg gcgaaggagt tctccgtgcc gcgcgcnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggac gcttcggaac ggtgcttgag
     6121 gccagagcca cgattttcaa agcgctcccg cttgctgacg acgcctggcg attgataccg
     6181 gaacagagtg gtggcgggcc actgatggac ctcggagcac acaccataga catactcact
     6241 ttccttctgg ggcccatgga gtcggcggta gggtttgcgg ctaatcgagc gacaaactgg
     6301 cgggtggaag acacggtgtc gctgttgctg cgagcgaggt cgggagcgat tgccgtggtg
     6361 gagcacagtt tttgcgcgaa gggcggcgat cagacgctcg aggtgaatgg aacggaggcc
     6421 agcgcgctcg tgtcgacgcc gccgccggga ggagaaaagc cattcctcag ggtcgcaggt
     6481 ccccagggtg tggtgaatga accggtgcct tttgagaatt attaccaact gcaaatcgag
     6541 cacttcgcgg aatgcctttc cggaaacgtc gctccgcttg caacgggcga ggatggtatc
     6601 cacaacctcg cggtgatcga agcggcgtat cgttcggttc agacgaacag ggtggaggct
     6661 gtgcagacat gaagcgatac gcgctccgtg gtgttctcgc agtggttctc gtgaccatgg
     6721 tcgggtgtgc tccgaaacgg gtcgagcggc cgcgcggtcc gcagcagcca cctatggttc
     6781 ggattcctgc gggggaatac ataatcggca ctgacgacgg cccggaaaac gaacgtcccg
     6841 cacacaaagt gcgagtccgt gcgttcatga tcgacaagtt cgaggtgacc aaccgggcgt
     6901 atctacaatt cgtgcgcgaa accgggttcc cggcaccgcc gcgctggctt cccgacagca
     6961 cgattttggg ttccacggat cgtttgccat tcgatgagcg ggaagccgat ttgccggtgg
     7021 cgtacgtttc gtggcaggac gccgatgcgt acgcgcgctg gcggggctgc agacttccga
     7081 cggaagcgga gtgggaaatc gccgcgcgtt gttccacggc gatgctctat ccgtggggga
     7141 atgattctct cgccacggac gggaagcacg cggcaaacac gggcggcgag agcgacggat
     7201 tcccggggct ggctccggcg ggcagttttg ggaacggcac atcatgctgg ggcgccgagg
     7261 atctcgtggg aaatgtgtgg gaatggacag cggattggta cataccggca gcgtacaggg
     7321 aagcttcacc gaccggattc gcggaggtgg tttcagatag tctctttcga cagcgggtga
     7381 tacggggagg ttcatggttc gatccaccgg ggcgtgtgaa agcgacgacg cgcactggtt
     7441 ttgacccatc tttccggagt gacattatcg ggtttcgctg tgcccgcgat tctgaatagg
     7501 ttctccccaa gatgaaggac gacgcaccgc tcctgagctt ctgggaccac ctcgaggagt
     7561 tgcggtggcg catcgtcaag tccatccttg cgcttctcat cggcggaatc gtttcgctgt
     7621 tcttttctga tcgcattctc gcgctcctgc tcctcccgct tgataaactc caagggaccg
     7681 ttcgcctcgt caatctctcc cccctgagca tggtcatggt tcgcctgtac attgccatca
     7741 tcgcggggct tcttgtgggc ctgccggtga tagtgtacca ggcgtggtcc ttcgttgcgc
     7801 ccggtctgta tccacacgag cgcagggcgg taccctgggt tctgataagc acgtttctgc
     7861 tctttgtgat tggcgcatcc ctctcctata cgatcatgcc ttttttgttc agggtcctcg
     7921 tcgaagccgg ttatcagggc atcgagaatt tctggaatat tcgtgaatac atgggatttc
     7981 tgttgggatt catgggtgcc ttcggcgtcg tgttcgaaat gccggtggcc gtatacattc
     8041 tgtccgtcgt agggatcgcg acgccggcag gtctgcgccg atcgcggaaa tacgccatcg
     8101 tcctcatttt cgtcgtctcc gccgtgctga cgccttcccc ggacccgttc agtcagattg
     8161 cgatggcagt ccctcttgtg gccctcttcg aggtcagcat cggcgtatcc gcacttgtgc
     8221 accgtcgcaa aaaacgtcgc agagaagctg cagaatcgga aaacgctcag taggtggggc
     8281 gggcagtttg tccgcgtttg gtttatcggc gcatgagtgg tattttctac ttcttacgca
     8341 cgacgaatac ccttaccgca ccgcgttact ggagcaggga aatgggcaga atacttcctg
     8401 tccgtcttgg agtcaatctc gatcacgtcg ccaccgtccg tcaggcgcgg aaaacgacag
     8461 aaccgagtcc ggtgcaggca gctctgctgg ccgaactcgg cggagccgac caaataacga
     8521 ttcacctccg tgaggaccgg cggcatattc aggatgatga cctcgaactc atgcgccgcg
     8581 tgacgcagct tccgatcaat caggaaatgg gcgtgaccga ggaaatgctg ggtatcgcac
     8641 tccgaattca cccgaatgtc gcgtgcctgg taccggaaaa gcgcgttgaa gtcacgactg
     8701 aaggtgggct ggacgtcgtc ggcaaccgcg aacgggttgc gcgcgccatg gaacggctcc
     8761 atgcggcagg tattgcggtg tcattgttta tcgatccgga ccctgcgcaa gttcgaattg
     8821 ctgcggaact gggtgcggaa gcgattgagc ttcatacagg cgaatacgcc aactcccggg
     8881 atgcggaaca ggccacacaa ctccaccggc tggaagctgc cgccgttctg ggatgtgatc
     8941 ttggtctgga agtccacgcg ggccacggcc tgacatacca gaacgtgtca cgtgttgcgt
     9001 caattccgca gatcgtcgaa ctcaatatcg gtcattccat tatcgctcgc gcgttgtacg
     9061 taggtctcag gcaggccgtc gccgaaatgc gcacgctgct tgccgaggcc cggaatgagg
     9121 cgcgcctgaa ttccttgtct gacaagtaag gagttgccac gcatgccacg tgggttgcag
     9181 atcagaattg ccctgttgct ggtgataatt ggctgggctg tctaccagct ttacccgacg
     9241 ttcgtgttct ggccgctgac ggtgcaacag cgccaggctc tgcagttgac cgacaaggcg
     9301 aagttcgatg atttgcagat gcgcgcgatc aaacggggcc ttgacctgca gggaggtgtt
     9361 tttctcgtac tcgaggtgga cccggcacgc acgatgaccg gagggacgct tgcggacgcc
     9421 gtggaaggag cgaggcgcgt catcgaacag cgcgtaaatc aatacggcgt ttctgaagcg
     9481 aacgttcaga aaatcggtga gcggcggatc atagtcgaat tgcccggtct ggacaacatt
     9541 gaagccgcga tgcgccttgt gggacgtacc gcgcttctca agttcaacct cctgaggccc
     9601 gtatcagaac gcgacgaggt tgtgaaacgt atcgacgcag ttttgcccca actgctttcc
     9661 ggggtagtaa tcgacaccac agcttccgtt gattcctctg ccgccgctgc cgcaacaccg
     9721 gcatctcgcg cggctacgga ttcaactccc gcatcgcaag gcgagttgtt cggcggtgcc
     9781 cggcagccac agaccgccca gacaggcgtg gcacgagccg actctgcacg cgagaatccg
     9841 ctcaaaggac tcacatcgtt cagcggcttg ctgaatgtcg ggccgggcaa cgactactac
     9901 gtgtccacgg tgaacaagcc gaaagtcgaa gcagtattgt cattgattgc cgaaaagaac
     9961 cccggcctta ttccctcggc gggggagttt ctgttcggca aggaagaatt gatcaacggg
    10021 cagtacgtaa ccccgctcta ctacgtctct tccaacgccg agatgaccgg cgccagcgtg
    10081 aaggatgcgc aggtctccca ggggtccaac ctcgacattg aacaggcggg acgtcccgtc
    10141 atcaatttcg aggtaaaaga ggattcggtt cgcctgttct cagcgctcac ccggcgcaat
    10201 cgtggcgaac gcatggcaat cattctggac aatgtggtgt attcagcgcc aaccattcag
    10261 gcgcacatca ccgacgggcg atccatcatc accggcatgg cggacaagga ggaggcgcgg
    10321 agtctcgcta ttgcgattcg gtcgggagct ttgcccgccg aggttcaggt ccaggaacag
    10381 cgctatgttg gaccttcgct cggaacggat tccatcgaat caagcctcag agcgtcgatg
    10441 atcgcgggta tcgcggtcat tgccttcatg gtgttgtact atcgcctggg cggggcgatg
    10501 gcggtggtca gccagacact gaacctctgc ctggtcatgg ccagccttgc ggcgttgcat
    10561 ggaacgctga ctctgccggg cgtcgctgga ctgatcttga cgctgggaat ggcggtcgac
    10621 tcgaacgtcc tggtttttga gcgcatgcgc gaagagagca acagcgggaa atcaccgcgc
    10681 gcgacaatcg aatccggctt caaaaatgct tcgagcgcca tctgggattc caacatcacg
    10741 acccttatca gtgcgatcgt gctgtaccag ttcggaaccg gtcctgttcg cggcttcgcg
    10801 ctcgttcttg cgatcggtat cgggggtact gtcttcgccg cgctgttcat cacccgcctg
    10861 ttctacgatc tgtatgcgta tttcgggagg ctccgtacta tttcactagg aatacccttc
    10921 ctgaagaatc ctcacttcca gttcctgaaa tggcgaaaac cgtggttcat tggctccggt
    10981 attgttctgg ttgtcagtgt ggccagcatt ctgaccgtcg gcttcaactg gggagtggat
    11041 ttcacgggag gtactcgtct cgccgttcgc ttcgttccac ccgtcaaacc cggcgacgtc
    11101 agaactgctc tcagacgggt tcccgttggc aaccgcgtgc tggatctctc acagagcgaa
    11161 ataaaaaccg ttgacgaggc aacggatctt ctcataacag tgaagcagat ttccaacatc
    11221 gaagcggctg cagtggacag cgcgttgcgg attcgacttt ctaccgcctt tcccgcaaac
    11281 acaaaagggg attgggtgct gaacgcggaa tccgttggcc cgaaggtggg gtccgagctg
    11341 aagagctcgg ccatctacgc ggtgctgttc tctctgatcg ggatcctcat ctacctgtct
    11401 ttccgtttcg agtggatcta cgcagtcacg acgacagcgt cgctgtttca cgacgttctt
    11461 ctcgcgcttg gcatttttgc gctgatccgg cacgaagtca cgctgtcagt ggtagcggca
    11521 gtcctgacga tggtaggata ctccctgaac gatacgattg tggtatttga ccgcattcgt
    11581 gaaggcctgc gaatacacgg cagaaggctg acattcggcg agatactgga caaatcgatc
    11641 aacgaaacac ttacacggac catcataacg agtggcacca ctcttctggc cgtcgttgcg
    11701 ctgctcattt ttgccggaca gaccctgaaa gacttcgccc tcgttctgtt catcggggtt
    11761 ctcaccggta catattcgtc catctacatc gctggcgcgc tggtgttcga gtggcagaac
    11821 cggcagcaga gccgggcggc cgagacggcg aggaaacggg gcgcttctgc ggtcaacaag
    11881 acaaaaaaga gcgccacggc gtaggcggcg aacaacgggc gtgcccgcct ctgcaactat
    11941 ggcgtaactc tgtctttttg gcgcaactgt ctcgcttgtg gttcatcggg catgtatttt
    12001 gcccttttga ccctctcgat tttccgtgtt gcttacgcag cgttgcgggt atgatgtgaa
    12061 ggaagggcat ctccggcaat cggcccgaaa tggttaatta gaaagggaat tgagctcgat
    12121 atgcctacct acgaatatca gtgtgaatcc tgcggtcaca gatttgagcg gtttcaatct
    12181 attaaagccg atcctttgtc cgagtgcgaa atctgcaagg ggaaagttcg gcgactgatc
    12241 tctgcaggcg gcggcctcat cttcaaggga tcgggtttct acattacgga ttaccgcagc
    12301 gaaggttaca aggcacagtc caagcgcgat aacgggggtg attcgagtac ctcctcgacg
    12361 gtgacgtcaa aagaaaccac ggcgaaaacg acgacgtaac gtggccatgt cgcgtgcagg
    12421 aaaaagcact tctcctctcg catgcggtct tccccggaga gaaagagaaa tgaatcgggt
    12481 atccgtgttg gcaacgtgtg cgttgttcgc ggcaacgatg atggcctgcg gacgcagccc
    12541 ggaggcgttg ttgaaaaagg gcaacgcggc ccttcttgag acgaccaaac cgcaacccga
    12601 caccgcaacc atcgatgacg gggtgaaggc gttgcgggag ttcacaacca gacatatggg
    12661 tcacgagcat gcggacagcg cgtactacct tctgggcatg atctacggcg cgacgcagag
    12721 gaatgcggaa gcgggggaaa ccttcatgac actggtccag aatttcccca ccagtcggtt
    12781 gcgtccgaaa agcctgatcc tggcgtcgca ggcgtacgaa aacgtaaaaa aatacggcca
    12841 cgccaagcag tgcatgcaga ctcttatggc ggaatacccg gaccacgagt tcgtaaaagg
    12901 cgggtcagcc aaatggcttt tcctgaacat cgggaaagaa ccggaagcgt gggatcagag
    12961 cttcatctcg gactcatcaa cggcacgcgc gtcgacccgg cccaggtgag ttacactggt
    13021 gttgtaccgc acaggggtat tttgcacgta ccattgctct cgctgtgcgt agtgcttctt
    13081 ctcgccgtcg gagacgcgtg gccggcggat gacatacgca tcgaggcgca actcgaccgg
    13141 ggtaccgtcg cgcttgacga aacggccacg ctggtggtaa aagcggaatg ggaggggcct
    13201 tcggatagat tcgcactcgt aataccgcag gcaccgcgtg tccacaacct gatcgtgatt
    13261 ggtagtaaac gtggaacgcg ctcctgggcg gatgagaagg gggaacactc tgcgcaggag
    13321 ttctcctacg ttcttcagcc ggaatctgaa ggaaacgcgg ccgtcggtgc gattcgcgtg
    13381 caattcacca cgcctgaagg cgcgtcaggc gatctggtaa cccaaccgtt gcagttgcgc
    13441 gtcggaagtc ccgtgcacac gcggtggtgc ttttcgtggg gagatgtcgg aggcctcgtt
    13501 gtcctcgcgg gtctggccgg atatgcggga tggcgccgct ggaagaagaa aaccgtgcgg
    13561 gaaaccgcat cgttgagagg ccagaaggat ccgttcgcgg cggccctttc ggagatcgct
    13621 tctgcccgaa aagcaggaaa tcagaaagcg ttttacggtg ccgttctttc tctcctgagg
    13681 cagctgacga gcgaacgaac gggtgtcccg acggcggcgc ttggtgcgcg ggatctcgtc
    13741 ccgtttgcgg accgacttgt ttccgatgag agttccgcgg aacggcttcg tgatttgttg
    13801 agccgcatcg acactatcct gtatgctcca gcgacggatg atttggatgc ggacatggac
    13861 gtgacggagc tctttgtgag gaatttggtg agcgagaacc agatcgggaa aagggaacgc
    13921 aaggatgcct cttgatagcc acattcagga gattcatgcg gaaatcgaac gcgaaagtga
    13981 gttcgtagaa cgcatcgtgt ccgaaatcgg caaagtgatt gtcggccagc gcacgatggt
    14041 tgagcggatg ctgatcggac tgctttgcga cgggcatatt ttgctggagg gcgttcccgg
    14101 gctggccaaa acgctggcag taagcagtct ggcccggacg atacatgcga agtttcagag
    14161 gattcagttt acgcccgacc ttttgcccgc cgacgtggta ggaacacctg tctatgagca
    14221 gaagaagtcg acgttcacgg tgaagaaagg accgattttc gcgaatatcg tgcttgcgga
    14281 tgaagtgaac cgcgcacccg cgaaagttca gagtgccatg ctggaggcga tgcaggaaca
    14341 ccaggtgacc atcggcgacc agacattcgc ccttgaggat cctttcctgg ttctcgccac
    14401 gcagaacccg attgagcagg aaggaacata ccccctgcct gaggcacaaa ttgaccggtt
    14461 catgctgaaa gtggtggtaa cctacccctc caaggcagaa gaacgtcaaa tactcgacag
    14521 aatgaccgaa ggcgatgttc cgcacgtgaa tgaagttgtc gaaccggcgg acatcatccg
    14581 cgcccgtcaa ctggttcgtc gcatttacat ggacgagaaa atcaagaatt acatagtcga
    14641 gatcgtgtgc gcgacccgaa aaccggcaga atacggcctg gcggattaca ccgattttgt
    14701 cgattatggt gcttctccgc gcgcaaccat atggctcgct ctcgcgtcga aggctcacgc
    14761 atttcttcgt caccggggat atgttacccc ggaggacgtc aaagccatcg ggatggatgt
    14821 tctccggcac cgtgtcattc ctacctacga ggcagaggcg gaggaagtga ccgcggaagc
    14881 aatcgtcagc aaggtcttcg attccgttga agtgccatga tccagaagga aatcctgaaa
    14941 aaggtccgtc ggatcgaaat ccagacacgt caggtggtta acaccctttt gagcggcgaa
    15001 taccattccg cgctaaaagg gcaagggatg gaatttgccg aggtgcggga gtatcagccc
    15061 ggcgatgacg tccgcaggat tgactggaac gtgacggcgc gcatgcgtcg tccttttgtg
    15121 aagatattcg atgaggaacg agagctgacg gtgatgctga tggtggatgc atcgtcgtcg
    15181 ggtgattttg gatcggtaga gcagacgaag aaccagatcg ctgtggagtt gtgcgccctt
    15241 ctcgcgttca gcgccaccaa gaacaacgac agggtgggtc tcatagtatt cactgacaga
    15301 gtcgagaagt ttgttccgcc caaaaaggga cgaaagcatg ttctgcgggt gatacgagag
    15361 cttcttgcgc agttttcccg aacggaaacc agtgagccgc gacctttccg cagtcggccg
    15421 cacccagtaa tgggtggcct cgcaagtctg gcgattgtcg cggccgctgc ttcgatgatt
    15481 tcggcgattg gtatccccga gacgacctac cagtggcttg cggagacggt aatttccatt
    15541 ctgggtgccg ccggcgtggg cgtggtttcg tggtgggcat tccgcacgta cggggctgac
    15601 aagggacgcg gcaccgacat cgccggggcg cttgattttc tgaatcgcgt catgacgcgt
    15661 cgcggggtgg tcttcctgat aagtgatttc caggcggcag gttttgagaa agcgctgaag
    15721 gctgcccgac gaaagcagga tgtcattgcg atcacgctta cggacccgcg cgaggtcaca
    15781 ctgaagggag ccgggttgct ggctctggag gatccggaga caggagaatc cgtggttgtg
    15841 gattcgtcag gcaaacgttt ccgggactgg tacgaacagc aggcgcgaac aaggcatgct
    15901 caacggaaac ggctgttccg caccactggg gttgattgtg tagacgtgtg gacggacacg
    15961 tcctatgtgg accctcttgt gaggttcttt tcggcgcgtg ccaagcgaat gtgaagctcg
    16021 cgtttcctgc gaccggcgcg gcgtgttgtc tgcgacgtgg agaacgacct gcttttcgcg
    16081 tgtgtgaagg gtgcgaacgg cgggtaccga agggagatct ggatgagata catcaggggt
    16141 cttttggtga tcggcctggt tgccttcggc acgggctgtt caaaatctgg cgttgacccc
    16201 gagaagcagc gtgtttttgc gaccgaactt ctgaaccggg agctctacgc cgaagccata
    16261 cgtgccttcg acaagtacct ggaaatgccg gccgtgtctg acagggaccg cgtggacgcg
    16321 atgcgcaagc tggcagacgc gctgttcgac agagcgaacg attaccactc agcgctcgtg
    16381 tattacctca gattacgggt tttcgcccca gattatcctg agatgaatga agtccgcgca
    16441 cggcttgtca catgttttga acgaaccgga cggagtgctg acgcatccct gatgcggcga
    16501 gagattgccc aggggaagac tttgccgccc gattcgttgg cggggccggt cgtggcggag
    16561 ttcggaggca ggaagatttc ggaacgggaa gtgctgcgcg aactggaaca gcttcccccg
    16621 gaattgcgtc aacaattcag caccatggag cggaaacggg aactcctgcg ccaggtggtc
    16681 ggcagggaga tcctttatga gatggcagtg aagaggggat atgccgatag tcccgagttc
    16741 caactccagc ttgatcgcat caggcgcgat atgcttgttc agcggattgg tgaagaacaa
    16801 ctcgggagcc ttccggacat aacggaagcg gacgtgcggc gtttctacga agaacatcaa
    16861 gccgaactgc ctcgcgttcc gggtggaggc gttccaagcc tccagcagat ccgaccccag
    16921 atcgagatgg cggcacgcca ggcgaagcaa caggaagcct tccagcggct cgtggaccag
    16981 cttttcgcca gccaggaagt caagctgtac cctgaacgca tgagatgacg gaagattgtc
    17041 tctccggaaa tccggggaaa acgggaaaaa acatggatga gcttcactcg tttgcaacct
    17101 atccgacaca ggagggagcg aattgatcga agtccgaggt ctcaccaaga agtacgggac
    17161 attcactgcg gtggacgata tcagtttcac cgtggaaaag gggcagatcc ttgggttcct
    17221 tgggcccaac ggggcaggca aaacgacgac catgcggatt ctcactggtt acctgcctcc
    17281 aacctccgga actgcactgg ttgcgggcta cgatgtccag gaacagtcga tggaggtgcg
    17341 caggcgcatc ggttacctgc cggagtctcc gcccctttac aacgacatga ctgtccgtga
    17401 atacgtggat ttctgtgcgg cgttgaaagg cgtaccccga aataagcgtg cagaggccgt
    17461 gtctgccacg ctccagaagt gcgggctcga agacagggtc aaccagctca tagggaccct
    17521 ctccaaaggc tatcgtcaac ggaccggcct tgcccaggcg ctggtgcatg acccggaggt
    17581 gctcatactt gatgagccga cgattggcct tgatccagcc cagatcatcg aaatccggga
    17641 gctgatcaag gggcttgccg gtaatcacac ggtcattttg agcacgcaca tattgccgga
    17701 agtgagtgtg acctgccagg ctgtcgcgat cattcaccag ggcaagctgt gcgctgcgga
    17761 ttcactggaa aacttgaccc gcgtccacgg cgaatcgctg gaggagatct tcctccgtgt
    17821 gatcagtagc gacgtggagg aggctgcggc gtgatgaaag gaacatggac cgttttcgtc
    17881 aaggagctcc ggtcgtacgt gcactctccc gtcgcgtacg tcatggcatt tgtctttctg
    17941 attctgtgcg ggtatttctt ccaggacgcg atgaatttcc tcaatcgcta cacgactgac
    18001 tatcccatgc gatatcagca ggcccagcag atgcggcggg atccgcctcc tcctcccaac
    18061 gtcgacattg tggtgattcg ccggacgtac agtgtgatgg cgttcctgct gacgttcatt
    18121 atgccgatcc tgacgatgcg cctgtattcg gaagaatacc gtaacggcac gatcgaactt
    18181 cttttcacct cgcccgtgac accggggcag acgctggcag ggaagttcct tgcgtcattt
    18241 gcactgtacg cgggtatcct ggtgctcacg ctgaactttg tcgtgtttgc gtacctgtat
    18301 gcggaggagg gggtacggcc tgataccggg ttaatactca gttgttatct gggccttctt
    18361 ttcctcggcg cttcattcat ttcggtgggt gtattcgctt ccgcgttgac ggaaaatcag
    18421 atagtcgccg ctgtagtgag tttcagtgtc ttgctcatgt tccttgtcat cggtgcggcg
    18481 gcggactacg tcggcaacta ctcagccgcg gaattcctca gatatcttgc cgtcaacggg
    18541 catgcagact cgttcctgcg cggtgtgatt tcgcttcgcg acatcgtcta ctacataacc
    18601 ttcatcgggt tcatgttgtt gctcacgaac cgggtgttgg aatcgcgaag gtggaggctg
    18661 taaatggtgc gatcgattcc tatcccgttt attgctctgg tgggtttgct cctcgccgtt
    18721 gatggcatgg ttgcgtggct tgccagcgcc acggtgtggg gttgggggat tccctgggcg
    18781 gtcggaacag gcctgttgct gggtgcattc ctttcgcggc tggggattcg aaaatccgtc
    18841 gcgtttgccg ctgcgctcgt ggttttgatt gccctttgcg cgatcatcta cgtgaaaggc
    18901 gaggtgatcg gcgcattcaa gctcgacagc atcacggcta ttttgttgtt tctgggtctg
    18961 ttcgtgggag aagttggcgt gggcatcgca tcggtattga ctggccgggc gagtgcacaa
    19021 ggggccaact tctgggtgta cgtagtgagc gcgctcgcga ttgcagtggt gctgaactac
    19081 ctcacgaacc gccacgtgcg gacgacgtac gacatcacac aggcgcaact ggaaagcctt
    19141 tcagaccaga ccctggcgaa gatgaagggg ctgagaaagg acgtgcacct cattgcattc
    19201 atccgggact ctgatccaaa tcgtcccggg tacgcagaga tgctcaagaa atacgcggct
    19261 gcatcacgct acttcacatt cgagttcgtt gatccggaca aataccctga catcgccgct
    19321 cgggaaaaca ttaaccccaa cgacatcacc atcattgtca aatgtgagga ccggcgggag
    19381 ctggttcttg ggcccgagga gcgcgacctg acaactgcaa taatcaaggt gacgcgcccc
    19441 ggccagaaga gaatctattt ttcgatgtgg caccaggaac gcacgctgga aggagagatg
    19501 ggtatgttgg ccaatcttct ccgccagttg aattacgcga ctgcgccgtt gaagcttatt
    19561 gattccgacg ttcctccgga ttgtgctgtg ctggttattc ccggaccaaa agcgccattc
    19621 gcaccggtgg aaatcagccg gatcgaacgg tatctggcgg aaggtggaag tgctcttgtc
    19681 ctgctggatc cggattcgat cgaaaccggt ctggaaaacg ttgttgccag atggggtgtt
    19741 gttgtgcacc cgaacatggt catcgagcgt caacgcggac tggttcccta tgcaggcggt
    19801 ctgtacatgg gggaacagca gtctatgtac gcgcgtgcga tgaaatatga gccttcgcac
    19861 gccatcgttg ccgatctcga atcgaagggc attgcgacgg ggttctacca ggttcgggag
    19921 atcacgtgga caggcgcaaa ccctacgctg gcgtgtactg gcactccgat tgttttcgcg
    19981 ggctcacgaa cgtcgttcgc gatcacggag gtgaaagagg cgttgcgcaa cccgcggcag
    20041 gcgcacagcg cagagggcaa gccgtcgggt cccttctcgg ttgccgtggc ggcgaccgct
    20101 cccccgtcgg tgtcgaccgg cgtggaaggt gcaaaaacgc ggcttgttgt ggtcggcgat
    20161 gcagatttcg cgaccgacag ggtcgtgagc caggaacggg gcaacgtgga tttcatgctc
    20221 aactgcatta catggttgag cgaagacgaa gacctgatta cgatccattc aagggagccg
    20281 ggctacaaac ctatcgtcat gaccgcgagc caggggactt tcatgttcct gttctgcgtc
    20341 tggcggtacc cggcgatggt gttcgttgcc ggtctcttga tctggtggta ccggcggagt
    20401 cgtggccccg gcaaggataa ttgaacgcag aaaaccggag ggggatcagc ggagcggggt
    20461 gcgaatgaat cgcaccccgc gacctgttga cgcgatctct gaacgcgcgg tccatgcact
    20521 aaggcggaga agaacggcat gaagaagtgg atcaggacta tcattctggt cgtaatagcg
    20581 ctcgcgctcg gattctgggc gtattttggc gagcgtcatc tggataagat cgcgctggaa
    20641 aagcgtctgg agcagaaaat cacgcacatc ccgacggaaa agatcgcggg gttcaccctg
    20701 attatcaaca atccgaatgc gccgaaacct gatacaacgg ttgtggagcg agtgggctcg
    20761 gatgagtggc agatcgtcca cccaatcctg tggccgggcg agagcacgga atggcgtgct
    20821 ctcgcgcaga acctgccaag agcctggaaa cagcgggcgt ggaaatcctc tcctgacagt
    20881 gttgcattct tcggtctgaa gcctccactt gttcggctga tcatcaagcc tgtagacagc
    20941 gcgatgcgac cggacactct ggacataggc aaagccacac cgaccgataa cttctgttat
    21001 gtaaaatatc ctcggagtga ctcaatctat acgacagacg tcattcttgt ttcttccacc
    21061 ctgaaatccc tgcacacatt gcgagacaag tcagtgacgc ctattgaacc atctctcgta
    21121 cagcgagtaa ccgtgaagag gggtggcagg gactcgatgg tgatcgaacg ccgcggcaat
    21181 ctctggtgga tgacctctcc gttggaacgg tcagtggatc gggacaccgt gaacgcgctg
    21241 ttgaaagaag tccagatgtc ccgtgtggcg gtcttccacg atacgcctgc tgcgatggga
    21301 acgtacggtc tggatcgacc tgtggcagag gtcgagttcc tcatggcata ccagggacgt
    21361 ctggccagac ggacactgct tcttggttcg aaagttccaa atg
//
LOCUS       DAOSOO010000031        20798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2553_length_20798_cov_6.016295, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000031 DAOSOO010000000
VERSION     DAOSOO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 20798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20798
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_20798_cov_6.016295"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLT86_03875"
     CDS             <1..297
                     /locus_tag="PLT86_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT35787.1"
                     /translation="FEPYILGYEIGNAYSEMNDPVRQRQLLEAQAAHREVDGEVPPVD
                     EDFLRAMEIGMPPTGGLGIGIDRMVMVLTDQASIRDVIAFPTLRPADARDADTP"
     gene            317..1849
                     /locus_tag="PLT86_03880"
     CDS             317..1849
                     /locus_tag="PLT86_03880"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOT35788.1"
                     /translation="MVKTPEAAYEELVQRVKNIALLGNTASVLHWDMETYMPSAGAEY
                     RSQQLSLLSGMLHEQFTAPEIGDLLTCVEQSDLVKDPLSDYAVNVREIRREYDREVKV
                     PKSLVEELARVTSLAHVVWREARKKSDFKAFQPTLENVVRLTLEKADAIATGPTRYDT
                     LLDGYEPGMTTDRMKAVFTALGNELNPFVEEILSSGKEPDRTILTRKYPTDRQAVLGQ
                     ILVGAIGFDLAGGRIDVTTHPFCAQVGLGDVRVTTRYNERDLGDAMFGLLHEAGHGLY
                     DQGTDPAHFGLPRGGFVSLGIHESQSRMWENFVGRSRGFWHFALPIARSVFPEALSDV
                     SLDAFYWAVNDVQRSYIRVEADEATYNLHVLLRFEIEQAIVSGDLAVADIPGAWNEKM
                     QQLLHITPRNDAEGCLQDTHWSGGGFGYFPTYTLGNLYAAQFFEQARADLGDLDTQFR
                     RGDFLPLREWLKEKIHRQGMRYRANELVEKVTGKPLSHEPLMRHLKNKLAPLYGAARE
                     TR"
     gene            1846..3102
                     /locus_tag="PLT86_03885"
     CDS             1846..3102
                     /locus_tag="PLT86_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT35789.1"
                     /translation="MNYEFFVARRYLRPKRSELFVSLTTMLTTLGVTLGVAALIIVLS
                     GMNGFAGEILRHLIGMNAHLWVQSQTGSLADSRGIEAKIKQIPGVVGVSPTIYEETLI
                     ANPSVAQTGAQIKGVDTLTIGDVSNIVRLFKEDSVNCTGRFFLGRDSANAEPGIVLGW
                     QLAGKLQVFVGDRVYVVIPPKGPVNPMMIVQPRFAPFRVTGVFDSGFYEFDATLAVMD
                     IHEAQRLFDYGTSVGAVEVKLRDAFMADGVDSLVSQKLGGYPYTTTTWVELNGGLYKW
                     LMIEKWFAFIVLSLIILVAAFNIVSTLTMVVRDKTKEIGILKAMGTTASEISRIFLVQ
                     GLIVGFVGSISGGILGYTLCWIQDKYHVVRLPAELYQVNAFPVEMRVLDFVAIGSAAI
                     VICVLSALYPARRAANMDPVEAIRNE"
     gene            3095..3859
                     /locus_tag="PLT86_03890"
     CDS             3095..3859
                     /locus_tag="PLT86_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35790.1"
                     /translation="MSDDLPVASEVAVLAENLHKSYPTPEGDLPILVGVDLRVRKGEI
                     VAITGESGAGKSTLLHLLGGLDRPTSGQIIVDGVRIDNTDEGARSRFRNERVGFVFQF
                     HHLLPDFTALENVMMPCLIAGTDVAAAKAVALEMLTSVGLENRLGHRPNELSGGEQQR
                     VAVARALANKPAIVFADEPSGNLDYRHSRQLNDLLWSLARAKEVTFVIATHDRALALR
                     ADREIRLENGVARELTKAETASYSEEIGNVASACRG"
     gene            3868..4407
                     /locus_tag="PLT86_03895"
     CDS             3868..4407
                     /locus_tag="PLT86_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOT35791.1"
                     /translation="MGKRVCERCKADDAIVRLISVKENTETVEYLCANCARAEGVEIV
                     VGGKEAVQVSDSGETEEVEQGSREDSVMCPGCRLTYARFKERYRLGCAECFDAFRPLL
                     MPLLKKVHGAERHRGKRFARLADEDITSGTPRGTWMLELLKRRLQAAVEREEFEEAAR
                     LRDRIEAISKESNVERTAQ"
     gene            4404..5483
                     /locus_tag="PLT86_03900"
     CDS             4404..5483
                     /locus_tag="PLT86_03900"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOT35792.1"
                     /translation="MTLDALVRQTPMWLDASGAYADVIVSSRVRLARNLRHIPFSSQN
                     KTADEERVVDEVLSAAQHVRDLNQSTYFDMKQLEEHDRQLLVERHLISPALALRGGNG
                     GVMVDQDERLSIMVNEEDHLRLQALTAGFEPQAAWQMLDKVDTELCGRLDIAYDADLG
                     YLTACPTNVGTGLRASILIHLPGLVLVQTVDQVLNGIAQVGLTVRGFYGEGTEVVGSL
                     FQISNQITLGKSEHEILETLERVVRQIIDFEYSARQTLLRDARAQIEDKIWRSWGILA
                     NARVLSGQEFMNLASAVRLGVSTGVLKEPSLVLLNELLVRVQPSHIQRMAGSRLDSDQ
                     RDQNRAKYVRERFASTTTSSRQTRD"
     gene            5532..8045
                     /locus_tag="PLT86_03905"
     CDS             5532..8045
                     /locus_tag="PLT86_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT35793.1"
                     /translation="MDHQMFTERVKKVMALAREESQRLGHDYVGTEHLLLGLIKEGEG
                     LAIAALTSLGLDPETIKQSVEDAVAQTGGQMLMGQAPFTPKAKEILERAMAEAKEMKV
                     QYVGTEHLLLALVKDRENVAAQILSALGADYKTIKEEVIALITGGSKAGQPKEKKSKT
                     PFLDHFGRDLTKLARENALDPVIGREKEIERVSQVLSRRKKNNPVLIGEPGVGKTAIA
                     EGLAQKIVAKEVPHVLENKRIVTLDMGGIVAGTKYRGQFEERIKAIMNELVQNREVVI
                     FIDELHTIVGAGSAEGTLDASNLFKPALSRGELQCIGATTLDEYRKHIEKDGALERRF
                     QTIMVDPPSVEDTVKILKGLRPKYEEHHRVKFPDATIEAAVMMAERYLHDRYQPDKSL
                     DVIDEAGSRVRLSKVSVPNEIKALEAQIAEIETKKESAVNEQNFEEAANLRDEEKALR
                     DKLALERRKWEEARENDVIEVTEEDIAYVVSSMTGIPVFRLAKNESEKLMHMEEELGK
                     RVVGQREAIAAVSRAIRRSRSGLKDPDRPIGSFVFLGPTGVGKTELGKALAAYLFEDE
                     DAAIIIDMSEFMEKFAVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEKAHP
                     DVFNILLQVLDDGRLTDSYGRKIDFRNTILIMTSNLGSRDIGSMGTFGFGSKDSLPSR
                     SEMESAINRELKRAFNPEFLNRLDEVVIFNTLSHDDMLTIVHILMSDVKKRVKDRHLD
                     LEITPAATVFLAEKGYDPALGARPLKRTIQKYLEDPLSEEILTGKFDGGGTVLIDRDG
                     DRLVFSLKDVLPAPAQNPNEPGIEDHGTADPLVGNTQTR"
     gene            8243..9268
                     /locus_tag="PLT86_03910"
     CDS             8243..9268
                     /locus_tag="PLT86_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35794.1"
                     /translation="MPKKVRVAMMSMAHAHADGYARQVGQIPDAQLMCIWDDMPERGK
                     PMAEKYGVPFFGNAMEEVLDRKDVDAIVVNAPTAQHTEIYLKAIERGKHVFTEKALTI
                     STKEADVVVDAVKKHPGIKFTVSLPSRTRGENLLIKELVEDGVLGRITMIRGRVAHQA
                     AIDKWFSGGQLWFVDKKAAGGGAMFDLGCHTTDMIRWIMGPPRYAVAQMNNFSRVYKI
                     DDNGVAVVEFANRGLGILDTSFCHRSGPNLFEVFGTEGYVGRGFPGMPLMIQSRKLKG
                     GNKIDGMIIPSKLPENKPSIMEAWISAILKDTPLITTVEDGRNLTEMLEGCYTAWRTG
                     KRYTFAK"
     gene            complement(9305..9907)
                     /locus_tag="PLT86_03915"
     CDS             complement(9305..9907)
                     /locus_tag="PLT86_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35795.1"
                     /translation="MRWTSWLPYAVIAAAVTAAAVNLPPSEMERSVAEGIVHSAAQSP
                     FWLFVGGALSDDFVSSAAIALTTLLLYVFLPTKESVIPLAALGGAAVLDRLLKGIMFS
                     STGASVPDVPSSAVLLASAWAGSVALVVARTGRMTRWRKAALTGAVLVVLLSGASALI
                     AKRSWPMEILAAWLWAAAVVLWLHRATRNVLTGKGIREGT"
     gene            complement(9904..11190)
                     /locus_tag="PLT86_03920"
     CDS             complement(9904..11190)
                     /locus_tag="PLT86_03920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT35796.1"
                     /translation="MSTSAASTGFPDSESVSRTVLPDGAILLVRENPASPAVVVQGAI
                     LAGAVDDPLDRPGLCRFTAECLTRGTQRRSAAELFELVESVGGSLGVGAGLHTIRLGA
                     KCLSDDLQMIVELLSEVLHSPSFPAAEIEKVRGEVLTELEERDNDTRSVTDLAFRKLA
                     YPEDHPYSRPADGERETVTGFTRDDVQHFFDSLVRRRPAIISVVGNLRQDDVEKLFAP
                     LWSTNARTANPAPLPDCTRRDGRRETYTVIPGKVQIDLAWGTIGPPRNHPDFVPASVA
                     NLILGGFGLMGRLGQKVREELGLAYYAASRVNAGLGAGPWVCVAGVAPEHYKKAVSAI
                     EQEVAKLREIPVPQDELEDCKSHLIGSLPLKLESNEGVASIMSEIEIYRLGWDYLHRF
                     ADLVNSVTAEDINRVVRAYFEPDQAILAGSGPEVPG"
     gene            complement(11187..12518)
                     /locus_tag="PLT86_03925"
     CDS             complement(11187..12518)
                     /locus_tag="PLT86_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT35797.1"
                     /translation="MANPPYQVSVLTNGLTVVTEERHHAPVTSFWMGYRVGSRNELPG
                     QTGVSHWIEHMLFKPSQRFPGIERDRLIAREGGTTNAFTWIDCTAYYTTIPSSKLDIV
                     LQIEADRLSGPLFAPDVFETERSVILAERDAYENQPEWRLAEQVTAAAFHAHPYGHEV
                     IGIAEDIRNLSREDLLAYFRTYYIPNNAVCVVVGDFETRVLLGRLEELFSEIPPGATP
                     PAPRAIEPAPVAERRVLLEGPGATGYLEMAFLAPKATDPRFVPSLVLGSILGSPISLG
                     FGSGAESRSSRLYRALVEKELAIDVDCNLEASIDPFLFDITAVVRDGRTHGEVEGAIL
                     RELEAIAASPPDAAEMAKARKQLRALFAFTNERITHRAMVLTLGFTVFPVQFLDEFEQ
                     RISDVTAEDVRAAAESLFARRNRVVGWYQPLGGEDELEDADEETETEEGIQ"
     gene            complement(12537..13022)
                     /locus_tag="PLT86_03930"
     CDS             complement(12537..13022)
                     /locus_tag="PLT86_03930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35798.1"
                     /translation="MSEPVYEIAGVEAVDEAVKVHEAAFYEWDGAKDLQKGAASWRQT
                     LTEKPGALLTVARLDGRIVTYVLGHDAQEPGVFFIALMGGLPEIRGKGIGRGLFRFTL
                     EEIRRRGYRSVDINTLNRYRGMLTLCITEDFDIVDLRFDQGRACNRIYLRRVFGPHVR
                     P"
     gene            13187..13996
                     /locus_tag="PLT86_03935"
     CDS             13187..13996
                     /locus_tag="PLT86_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT35799.1"
                     /translation="MDKGQEQLMCDNTDIMESVKEIAEEAGRIALSQFGASRPHVKTD
                     RSFVTATDREVEQFIHRKLHELFPDDAIIGEEFGGSPGTIREYVWLLDPIDGTTNFVA
                     GLPHWAVCVARLRFGVPVLGVVVLPVLRETYAAATGIGATLNGSPFKMLERDDPPNEQ
                     LIAVWSTWYREVDLKFSGKVRTLGSTVVKCLYAAKGSFVGALTPEVHAWDIAAGLPVL
                     WESGGQAYARDGKVYEVMDLDPANGYLVPPLILSAPSRRDFMRQMITARRT"
     gene            14019..16115
                     /locus_tag="PLT86_03940"
     CDS             14019..16115
                     /locus_tag="PLT86_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35800.1"
                     /translation="MRLSRSSRIPLIVFVAVHSFAGCAYYNTFYNAERAFEKGERARQ
                     SLEALQRTGSPATRRPSQQSSSTRGAAVGDEQSYRTAIEKSAKVLVYHPRSRYVDDAL
                     LLMAKSYYRLGEYAPSLRKCTELLTTFPQSPLAPEARYWEGMALWQLGRYEEATGVLS
                     RIAADDGSRFQGDAAFALAKLKRERGSSEEAIPLYRVAVRRSTEREFRYRARIELAEC
                     LIEAGGMSEAVEVYRELSRLPQTAAERYENLLRIARTQRAMGNAEAALATLEPLIRDQ
                     RFTDQVPRTRLEIARTKEAAGEIEAAVLLYQKVLEDAERLSPPTVTQAGPPGSGPAGG
                     AAPTGVRTRETAEANFHLGRLQEQRFRNFANAARHYALAAQSPDQEVALPAKRRMDDI
                     SLWTQLHATLADTTDSVSVSRKDRARYSLAEHHLLAMDEPDSALFYFDAVAERTSDSD
                     LRMRAQYASGWIWREVKRDTARSDSIWQKLITDSSRTRSTMEIKRAIHSVQCRPEQEP
                     GIDLYQEAEGRWFDSLVRCPPYPPDSVTKDSLSSATWWDEWRACEKRECESAIKDFNR
                     LLELYPQGNLATRARFILGWYAENVLEDTASAYRWYDAAREDSVFAPEVGRVADLLLK
                     SRVTGGTGRSAETAGIRDAAAADTTAELGQPLEPRTPREPGPEAPPQPEERSLERPER
                     VERVPVPRREPRRHAE"
     gene            16105..16938
                     /locus_tag="PLT86_03945"
     CDS             16105..16938
                     /locus_tag="PLT86_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT35801.1"
                     /translation="MLNSPLPATVPVDEAARVTEQAAVAPGFFRLRFHAPHIARHAVP
                     GQFVQVRVTDDLEPFLRRPFSIGDADPSRGLIEIVYHVVGKGSRRLSERKAGETIPLL
                     GPLGNGFRLPGCGTVVLVGGGVGMPPVYFAAKSIRGCHVCVIQGARCAERLLYREELS
                     SLGIPHRETTEDGSAGSQGLVTAALIETLSDFPPDERKGPVEILACGPPPMLAAVAEI
                     ARAHGVRCQVSVEERMACGFGICMGCAVQKRTAGSKEYALVCVDGPVFDATEIALAPS
                     S"
     gene            17076..18017
                     /locus_tag="PLT86_03950"
     CDS             17076..18017
                     /locus_tag="PLT86_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35802.1"
                     /translation="MLFLRIRLCPSIRNVEPDRWDYDSLRDALRLIGRWYRFVSMDEA
                     VSALAGRSPLFKSSIAVTVSIGSSLPEIDLGRLAIECDVPLTVFFCPGAVEAGVHPWY
                     CVLAFALNGLDGQTVTVGGRRWLLSNYDYRQLAFADIARQIASLPLTDRYRYTEELVS
                     GWRAKPAGSEVITFREMMRMMRSKRITFGVSGYSGDSLMRLPVERALYEIREGFALYR
                     RVFGEEPAHFEYPWGDRSVYLEEFVRNSGYRSGTLGLNAMDGLNRIGADPFRLAAQNL
                     RPRTPARIRADLAGVSKQLRPLERFLDVRTRRADRDF"
     gene            complement(18028..18783)
                     /locus_tag="PLT86_03955"
     CDS             complement(18028..18783)
                     /locus_tag="PLT86_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOT35803.1"
                     /translation="MRGTAAFCRLHYSVGNAAAALEYREIMPLIQSALADAGLLARNP
                     DGTTPKLRFGPPLPAGCSSMQEYCDAELDSTLDDVLSRINSRLADGLQCVQCDRIDRK
                     AEPVDRIAFYAEYSFEIPHSLLRECDVKALRELFDEQGEWLAERVSPKGHVRESDLKK
                     AVTNWRSESSASITTVYLRLAIAHEEGHTASPALVLQKLFDLNEEQSALVAITRREMF
                     DRSGAPLRSRPWPRKRNRLLHERCWEHQRFHHQ"
     gene            complement(18857..19483)
                     /locus_tag="PLT86_03960"
     CDS             complement(18857..19483)
                     /locus_tag="PLT86_03960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT35804.1"
                     /translation="MFSDLKLGFQVFVDPLVNHFIRRGYHPDTFTIMGLVFNTIAAGF
                     YALGMFFEGALVMLFGSSTDMIDGQIARKTGGVSDGGALLDSVLDRYSEAVVLIALSF
                     HYARIGWMITSVLSAAALTGSLLVSYVRARAEGLNYECKVGLMQRPERVIFLAAASVF
                     GVFLNTPYQHVAAAVWVMAILTNLTTCERIVHVRRAAKQREMSLREQR"
     gene            complement(19473..20198)
                     /gene="rsmI"
                     /locus_tag="PLT86_03965"
     CDS             complement(19473..20198)
                     /gene="rsmI"
                     /locus_tag="PLT86_03965"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT35805.1"
                     /translation="MTGRLYLVGTPIGNLGDVSDRVRAVLASVSLIAAEDTRRTRILL
                     KELGVSCPLTSYHDHNERTKFPELLSALEEGKDVAVVSDAGMPVIADPGWHIIQAAIQ
                     RGIEIIPVPGPTAFVLALVVSGLPVHRFAFEGYLPKKSSARRRKLEELAAEPRTMIFY
                     ETPHSVRSTLRDALEVLGDRKASLSRELTKKFEETRRGTLSTLLSSCDDKVLRGEFVL
                     VVSGTESVETRTPRAECGSEHVQ"
     gene            complement(20295..>20798)
                     /locus_tag="PLT86_03970"
     CDS             complement(20295..>20798)
                     /locus_tag="PLT86_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35806.1"
                     /translation="IFDRHTPESVQEYADLLFAGYVYRYDDPTDKNDLELDRASEIQV
                     YRNLFRSFDYISAHFAIENRWIEYGADMPYPPGTALYQISSAHATENWVVLRVLGDLI
                     FTNTDNNAVQTGYQVRQRFDIAFRLDTAKSDSTWQIASWSDRESLLNARIAVRPAENA
                     HVLSPRE"
BASE COUNT         4522 a         5749 c         6350 g         4177 t
ORIGIN      
        1 tttgagccgt atatactggg gtacgaaatc gggaacgcgt actccgagat gaacgacccg
       61 gttcgtcaac gccagctcct cgaggcacag gcggcgcacc gtgaagtgga cggtgaagtg
      121 ccccctgtcg acgaggattt cctccgcgca atggagatcg ggatgcctcc gactggagga
      181 ttgggcatag gcatagaccg gatggtcatg gtgctcaccg accaggcgtc aatccgcgac
      241 gtcatcgcgt tccccacgtt gcgccctgcg gacgccagag atgccgacac accctgacgc
      301 agaaacggag cgaccaatgg tgaaaacacc ggaagcagcg tatgaggaac tcgtccagcg
      361 ggtgaagaac atcgctctgc tggggaatac ggcaagcgtt ttgcactggg atatggaaac
      421 gtacatgccc tcggcagggg cggaataccg ttcgcagcag ctatccttgc tctcgggaat
      481 gctgcatgag caattcacgg cgccggaaat cggtgatctg ctcacatgtg tcgaacagag
      541 tgatctcgtg aaggacccct tgtccgatta cgcggttaac gtaagggaaa tccgtcgcga
      601 atatgaccgt gaagtgaagg tgcccaaaag ccttgtggaa gaacttgcac gggtaacctc
      661 gctcgcgcac gtggtatggc gcgaggcgag aaagaaatcc gatttcaagg cctttcaacc
      721 tacgcttgag aatgtggttc ggctgacgct tgaaaaggcc gatgccatcg ccaccgggcc
      781 caccaggtac gatactcttc tggatggata cgagcctggg atgaccacgg atcgcatgaa
      841 agcagtgttc actgccttgg gcaacgagtt gaaccccttc gtcgaggaga tcctttcctc
      901 cggaaaggaa cccgaccgga cgatcctcac ccggaaatac ccgacagaca gacaggcggt
      961 cctcgggcag atactggtgg gcgcaattgg ttttgatctg gcaggaggac ggattgatgt
     1021 gacaacccat cccttctgcg cgcaggtcgg tctgggtgac gtcagagtca ccacgcgata
     1081 taacgaacgc gatctgggag acgccatgtt cgggcttctc cacgaagcgg gccacggttt
     1141 gtatgatcag gggacagacc cggcgcattt cgggcttccg cggggtggct tcgtgtcgct
     1201 cggtattcac gaatcgcagt cgcggatgtg ggagaatttc gtcggaagga gccgtggttt
     1261 ctggcatttc gcattgccga ttgcgaggag cgttttcccg gaggcgcttt cggatgtgtc
     1321 gctcgatgcc ttttactggg ccgtgaatga tgtccagcgg tcgtacatcc gggtagaagc
     1381 ggacgaagca acgtataacc tgcacgtcct cctgcgtttc gaaatcgaac aggccatcgt
     1441 ctcaggcgac ctcgcggtag cggacattcc cggcgcgtgg aacgaaaaga tgcagcaact
     1501 gctgcacatc accccgcgga atgatgcgga ggggtgcctg caggacacgc actggagcgg
     1561 aggtggtttc gggtatttcc ccacgtatac gctgggaaac ctttacgcgg cgcagttttt
     1621 cgagcaggcg cgggcggatc tcggtgatct cgacacgcaa ttccgcaggg gagatttcct
     1681 gccactgaga gaatggctca aagaaaagat ccaccggcag ggaatgcggt atcgggcgaa
     1741 tgaacttgtc gagaaagtta cgggaaagcc gctcagtcat gaaccgctga tgcgccacct
     1801 gaagaacaag ctggctcccc tttacggcgc cgcgcgggag accagatgaa ttacgagttt
     1861 ttcgtcgccc ggcgctacct tcgaccaaaa agatcggagc ttttcgtaag cctcacgacg
     1921 atgcttacca cgctgggagt cacgctgggc gtcgcggcgc tcatcatcgt cctgtcgggc
     1981 atgaatggtt tcgccggtga gatacttcgc cacctgatcg ggatgaacgc acacctctgg
     2041 gtccagtcgc agacggggag cctcgccgac agccgcggaa tcgaagcaaa aataaagcag
     2101 atccccggcg ttgtcggagt gtcgcccacg atctatgagg aaacgctcat tgcgaatccc
     2161 tcggttgctc agacaggcgc ccagatcaag ggtgtcgaca cgctgactat cggcgatgtc
     2221 tcgaacatcg tgcgcctgtt caaagaagat tctgtcaact gcacagggcg cttctttctc
     2281 ggtcgagatt cagcgaacgc agagccgggg atcgtgctgg gctggcaact cgcgggtaag
     2341 ctccaagtgt tcgtcgggga tcgggtctac gtggtgattc ccccgaaagg cccggtgaac
     2401 ccgatgatga tcgttcaacc gaggttcgca ccttttcgcg taaccggtgt tttcgactcg
     2461 ggtttctacg aatttgacgc aacgcttgcg gtaatggaca tccatgaggc acaacgtctg
     2521 tttgactacg ggacttccgt aggcgctgtc gaggtgaagt tgcgcgatgc cttcatggca
     2581 gacggagtgg atagcctggt ttcgcagaaa cttggcggtt acccctacac tacgacgaca
     2641 tgggtcgagc tgaacggcgg gttgtacaaa tggctcatga tcgagaaatg gttcgcattc
     2701 atcgtactga gcctcatcat ccttgtggct gcatttaaca ttgttagtac gttgacgatg
     2761 gtggtgcgag acaaaacgaa agagatcggt atcctgaaag caatggggac aactgcgtcc
     2821 gagatatcgc gtatttttct ggtgcaggga ctcattgtgg gatttgttgg aagtatttcc
     2881 ggaggtattc tgggatacac gctttgctgg attcaggaca agtaccacgt cgttcgtttg
     2941 cctgcggagc tctaccaggt gaatgcattc cctgttgaaa tgcgcgttct ggatttcgtc
     3001 gcgattggtt ccgcggcgat tgtgatatgc gtgctgtcgg cgctttaccc cgcgcggcgc
     3061 gcggccaata tggacccggt ggaggcgatt cgcaatgagt gacgatctgc cggttgcgtc
     3121 cgaagtggcc gtcctggccg aaaacctgca caaaagctat ccgacaccgg agggcgattt
     3181 gccgattctt gtcggtgttg acctgcgcgt ccgcaaaggg gaaatcgtcg cgatcaccgg
     3241 tgaatcgggg gcaggcaaga gcacattgct gcaccttctt ggcgggctcg acaggccgac
     3301 ttccgggcag attattgttg atggcgtacg catcgacaat accgacgagg gagcgagatc
     3361 gcggttccgg aatgaacggg tcggcttcgt gttccagttt catcacctgc tgccggattt
     3421 cacggccctg gaaaatgtga tgatgccgtg tctgatcgcc gggaccgatg ttgccgccgc
     3481 aaaggcggtt gccctggaga tgctcacttc agttggtctg gagaaccgtc tgggacaccg
     3541 tccgaatgag ctttcggggg gtgaacagca aagggttgcg gtggcgcgcg cactggcaaa
     3601 caaacccgcg atagtgttcg cggatgagcc atcagggaac ctcgactatc ggcacagcag
     3661 gcagttgaac gacctgttgt ggagtcttgc gcgcgcgaaa gaagttacat tcgttatcgc
     3721 cacacacgac cgcgcgctcg cactcagggc agaccgcgaa atccgccttg aaaacggtgt
     3781 cgcgcgggaa ctgacaaaag ccgaaacggc gtcctattct gaagagatcg gtaatgttgc
     3841 gtcagcttgt cggggctgaa aggttccatg ggcaaacgcg tatgtgagcg gtgcaaagca
     3901 gatgacgcga ttgtgcgtct gatctcggtg aaagagaaca cggaaacggt tgaatacctc
     3961 tgcgcgaatt gcgcccgtgc agaaggagtg gagattgttg tgggcggcaa ggaggcagtt
     4021 caggtgtcag actccggaga aactgaggaa gtagaacagg gttcccggga ggattcggtg
     4081 atgtgtcccg gttgccggct tacgtatgcg cggttcaagg agcggtaccg actcgggtgc
     4141 gcagagtgtt tcgatgcgtt ccggcctctg cttatgcccc ttctcaaaaa ggtccacggc
     4201 gcggaaagac accgggggaa acgtttcgcc cgcctggctg acgaagacat tacctcgggt
     4261 acgccgcggg gaacgtggat gcttgagctt ctcaaaagac gcctgcaggc agcggttgag
     4321 agggaggaat ttgaagaagc tgccaggttg cgggaccgga tagaggcgat ttcgaaggaa
     4381 tcgaatgtgg agcgaactgc gcaatgacac tcgatgcgct ggttcggcag acgccgatgt
     4441 ggcttgatgc gtcaggcgcg tatgcggacg tgatcgtgtc gagcagagtg cgtctcgcac
     4501 gaaatctgcg gcacatacct ttctcgtccc agaacaaaac cgcggacgag gaacgcgtgg
     4561 tagatgaagt gctttctgcc gcccagcacg ttcgcgacct gaaccagagc acctattttg
     4621 acatgaaaca gctcgaggaa cacgatcgcc aactgctggt ggaacggcat ctgataagcc
     4681 ctgcactggc cctccgcgga ggtaatggcg gtgtgatggt ggaccaggac gagcgcctca
     4741 gcatcatggt taacgaagag gaccacctga ggcttcaggc ccttacggct ggatttgaac
     4801 cgcaggccgc ctggcagatg ctcgacaagg ttgataccga gctctgcggg aggcttgata
     4861 tcgcgtatga tgcggatctg gggtatctca ccgcgtgtcc gacgaacgtg gggaccgggt
     4921 tgcgcgcttc gattctcatt cacctgccgg ggctcgtgct cgtgcagacg gtcgatcagg
     4981 tcctgaacgg catcgcgcaa gtagggttga ccgttcgtgg cttttacggg gaaggaaccg
     5041 aagttgtcgg cagtctgttt caaatatcaa atcagatcac gctggggaaa tcggaacacg
     5101 agatcctcga gacgctggaa cgcgtcgtca ggcagattat cgactttgag tacagcgcgc
     5161 gacagaccct gctgcgggat gctcgcgcgc agatagaaga caagatatgg cgttcctggg
     5221 gcatcctcgc gaacgcgcgg gttctgagcg gtcaggaatt catgaatctc gcatctgccg
     5281 tgagactggg agtaagcacc ggggttctca aagaaccgtc gctggtcctt ttgaatgaat
     5341 tgcttgtgcg ggtacagcct tcccacatcc aacgcatggc aggaagcaga ctcgacagtg
     5401 accagcggga ccagaaccgt gccaaatatg tgcgcgaacg gttcgcgagc acgacgactt
     5461 ccagcaggca aacacgagat tgagaaacac tgaaaatcga taacaccgga atggaaggtg
     5521 gaggcaacgc aatggatcac cagatgttta cggagcgggt gaagaaggtc atggcgctcg
     5581 cccgggagga gtcccaacgc ctgggtcatg actacgtggg gactgagcac ctcctcctgg
     5641 gacttatcaa ggaaggtgaa ggtctggcca ttgccgcgct gacgagcctg gggctggacc
     5701 cggagactat aaagcagtct gttgaagacg cggtcgccca aaccggcggt cagatgctta
     5761 tgggacaggc tccgttcacc cccaaggcca aggaaattct ggaaagggca atggcggaag
     5821 cgaaggaaat gaaagtgcag tatgttggca ctgaacacct gttgcttgcc ctcgtcaaag
     5881 accgggaaaa tgtcgccgcg cagattcttt ccgctctggg agcggattac aagacgatca
     5941 aagaagaggt tattgctctc attaccggtg gaagcaaggc cggacagccg aaggagaaga
     6001 aaagcaagac gccttttctg gatcatttcg ggcgggattt gaccaaactg gcacgtgaaa
     6061 atgccctcga tccggtcata gggcgcgaaa aagaaattga acgggtaagt caggttctca
     6121 gccggaggaa gaagaataat ccggtcctga tcggtgaacc cggtgttgga aaaaccgcga
     6181 tcgcggaggg cctcgcacag aaaattgtgg cgaaggaagt gccgcacgtg ctggaaaaca
     6241 aacggattgt gacgctggat atgggtggca tcgttgccgg aacgaagtac cgcgggcagt
     6301 ttgaagagcg catcaaagcg atcatgaacg aacttgtcca aaaccgtgag gtcgtgattt
     6361 tcattgacga gttgcacacg atcgtcgggg caggttcggc ggaaggaacg cttgacgcat
     6421 ccaacctgtt caaaccggcg ctttcccggg gtgagctcca gtgtatcggc gccaccactc
     6481 tggacgagta ccgcaagcac atagaaaaag atggcgccct tgaacggcgt tttcagacga
     6541 tcatggtgga tcctccttcg gtcgaagaca cggtgaagat tctcaagggc ctccgtccga
     6601 aatacgagga acaccaccgc gtgaaattcc ccgacgctac gattgaagcc gccgtgatga
     6661 tggcggagcg atatctccac gaccgatacc agccggacaa atcgctcgac gttatcgacg
     6721 aagcaggctc cagggtgagg ctgtcgaagg tcagtgttcc aaacgaaatc aaagcgctcg
     6781 aagcgcagat cgccgagatc gagacgaaga aggaatcggc agttaacgag cagaatttcg
     6841 aagaggctgc caacctgcgc gacgaggaaa aagcactgcg agacaaactg gcacttgaac
     6901 gcaggaaatg ggaagaagcg cgcgaaaacg acgtcataga ggtgacagaa gaagacattg
     6961 cctacgttgt gagcagcatg acaggtattc cagttttccg gctcgcgaaa aacgaatccg
     7021 aaaaactcat gcacatggaa gaggaactgg gcaaacgcgt cgtcgggcag cgggaggcaa
     7081 ttgcggcagt gtctcgcgca attcggcgtt cgcgcagcgg actgaaagac cctgatcggc
     7141 cgatcggttc gtttgtgttt ctcgggccga cgggcgtggg aaagaccgaa ctggggaagg
     7201 cattggcggc gtacctgttc gaagatgaag acgccgcgat catcatcgac atgtcggagt
     7261 tcatggagaa gttcgcggtc agcaggctgg tcggtgctcc tcccggttat gtggggtacg
     7321 aagaaggcgg tcagctcacc gaaaaggttc gccgcaagcc gtattccgtc attctgctgg
     7381 atgaaataga aaaggcccac cccgacgtgt tcaacattct gctccaggta cttgacgacg
     7441 ggcggcttac ggacagctat ggcaggaaaa tagacttccg caacacaatc ctcatcatga
     7501 cgtcgaacct tggcagccgg gatatcggca gcatggggac cttcgggttc ggaagcaagg
     7561 actcccttcc ctcacgttcg gagatggagt cggcaatcaa ccgggaactg aagcgcgcgt
     7621 tcaaccccga attcctcaac cgcctcgacg aagtggtgat tttcaatacc ctttcccatg
     7681 acgatatgct caccatcgtt cacattctga tgtcggacgt gaagaaacgg gtaaaagacc
     7741 gccatctgga cctggagata acccccgccg caaccgtgtt ccttgcggaa aaggggtacg
     7801 atcctgccct tggcgcgcgt ccgttgaagc gcacgattca gaagtacctc gaagacccgc
     7861 tctcggaaga gatattgacg gggaaattcg acggtggtgg aaccgtgctg atcgatcggg
     7921 acggagatcg cctggtcttc tcgctgaagg atgtcttgcc tgcgccggcg cagaacccga
     7981 acgagccggg tattgaggat cacgggacgg cggaccccct tgtgggaaac acgcagacgc
     8041 ggtgacgcgc cgcacttgga gtatgagaaa cgggcggacc ctgaagggcc cgcccgttct
     8101 cttttcccct cggggcttgt gcagtgggga atgcgcggag tattgtttcg gttgttcttc
     8161 gggcgctgga cgcggcgttt ctggcgcaac atagccgatc gcaagtgccc ctgtgggtct
     8221 cagtctcacc aaggagaaaa cgatgccaaa gaaagtgcgc gtggcgatga tgagcatggc
     8281 acacgctcac gccgatgggt acgcgaggca ggtcgggcag ataccggatg cccagttgat
     8341 gtgcatctgg gatgatatgc cggagcgcgg caaacctatg gccgagaagt acggagtgcc
     8401 attcttcggt aacgcgatgg aagaggtact cgaccgcaaa gatgtagatg cgattgtcgt
     8461 caacgcgcct actgcgcagc acacggagat ctacctgaag gcaattgagc gtggaaaaca
     8521 cgtgttcacg gaaaaggcat tgacgatctc gacaaaagag gccgatgtcg tcgttgacgc
     8581 ggtgaagaag catcccggga tcaagttcac cgtaagtttg ccgagccgca cgcgcggcga
     8641 gaaccttttg atcaaggaac tggtggagga tggcgttctc ggtcggatca ccatgattcg
     8701 gggccgggtt gcgcaccagg ctgccattga taagtggttc tcaggcggcc agttgtggtt
     8761 tgttgacaag aaagccgcgg gcgggggagc catgttcgat ctcggatgcc acacgactga
     8821 tatgatccga tggatcatgg gcccacccag gtacgccgtt gcgcagatga acaacttctc
     8881 gcgcgtttac aagatcgacg acaacggggt tgccgttgtc gaattcgcca accgcggcct
     8941 ggggattctc gacacgagtt tctgccacag gtccggcccg aatctttttg aagtgttcgg
     9001 tacggaaggc tatgtggggc gcggtttccc tggaatgccg ctgatgatcc agagcagaaa
     9061 gctcaaaggc gggaacaaaa tcgacggcat gatcattcct tcgaaacttc cggagaacaa
     9121 accaagcatc atggaagcct ggatttcggc gattctcaag gacacgcccc tgatcacgac
     9181 agtcgaagac gggcgcaatc tgacggaaat gctcgaggga tgttataccg cctggcggac
     9241 ggggaaacgt tacacgtttg cgaaatgagg tgttggttct caaagtccgg gagaggaagc
     9301 tccttcacgt cccctcccgg attccctttc ctgtgagcac gttgcgggtt gcccgatgga
     9361 gccagagcac aaccgccgcg gcccagagcc acgccgcgag gatttccatc ggccagcttc
     9421 ttttcgcgat caatgccgac gcaccagaca ggagaacaac aagaacggcc cctgtcagcg
     9481 ccgccttccg ccatcgggtc attctgcccg tccgcgcaac aacaagagct acgcttccag
     9541 cccaggctga ggcgagtaac acggcgcttg agggcacgtc cggtaccgat gcgcctgtgc
     9601 tgctgaacat gatccctttc aggaggcgat ccagtacggc ggcgcctccg agggcggcca
     9661 acggaatcac gctttccttc gtcggcagga acacgtagag cagcaacgtc gtcaacgcga
     9721 tcgcggcaga ggacacgaaa tcatccgaca gtgcgccgcc gacgaaaagc cagaacggcg
     9781 actgcgccgc ggaatgaact atcccttccg ccaccgaccg ctccatttcg ctcggaggca
     9841 aattgacggc cgcggcagtg acggccgccg ctatcacggc gtacggaagc caactcgtcc
     9901 acctcatccg ggcacctcgg gtcctgaccc tgccagaatt gcctggtcgg gttcaaaata
     9961 cgcgcgaact accctgttta tgtcctcagc cgtcaccgag ttcacgaggt cagcaaatcg
    10021 gtggaggtaa tcccagccga gacggtatat ctcgatctcc gacatgatgc ttgccactcc
    10081 ctcgttcgac tccagcttca agggaagtga tccaatgaga tgcgatttgc agtcctccag
    10141 ttcgtcttgc ggaacgggta tctcccgcag cttggcgact tcctgttcta tggccgacac
    10201 agcttttttg taatgttcgg gtgcaacccc cgcaacgcac acccaaggcc cggcgcccag
    10261 accagcgttg acgcggctcg ccgcgtaata cgcaaggccg agttcctctc gcaccttttg
    10321 accgagccga cccataagtc cgaaaccacc gaggatgaga ttcgccacac ttgccggaac
    10381 gaaatcagga tggtttcgcg gggggcctat cgtgccccag gccaggtcga tctgcacttt
    10441 accggggatt accgtgtacg tttcccgccg cccgtcacga cgcgtgcaat ccggcaaggg
    10501 cgcgggattt gcagttctcg cattcgtgct ccagagcggg gcgaagagct tttctacgtc
    10561 gtcctgtcgc agattgccga ctacgctgat gattgccggt cgccgccgaa ccaggctgtc
    10621 gaaaaagtgc tgcacatcgt cccgcgtgaa cccggtcacg gtttctctct caccgtcggc
    10681 ggggcgtgaa tagggatggt cttccggata ggcgagtttc cgaaacgcga ggtccgtaac
    10741 tgatctggtg tcattatcgc gttcctcaag ctccgtgagg acctcccctc gtaccttctc
    10801 aatttccgct gcgggaaagg acgggctgtg aaggacctcg gaaagcagct caacgatcat
    10861 ctggagatcg tctgaaaggc actttgcgcc aagtcgaatt gtgtgcaggc ccgccccgac
    10921 gcccaacgaa cctccaacgg attccaccag ttcgaaaagc tccgccgcgg accggcgctg
    10981 tgtgccccgt gtcaaacatt cggcggtgaa ccgacacaat cccgggcggt ccagggggtc
    11041 atccacggcg cccgcaagaa tcgcgccctg aaccaccacc gcagggctgg cggggttctc
    11101 gcggacgagg aggatggctc catccggcaa cacagtccgc gaaacgcttt cggaatccgg
    11161 gaaaccggtc gacgctgcag aggtactcac tggatacctt cttccgtttc cgtttcttcg
    11221 tccgcgtctt ccagctcgtc ttcgccgccg aggggttgat accacccgac aacgcgattt
    11281 cgtctggcaa acaagctttc tgcggctgct ctgacgtctt ccgccgtaac atcgctgatg
    11341 cgctgctcaa actcgtccag aaactgcaca ggaaatacgg tgaatccgag agtcaacacc
    11401 atggcgcgat gggtgatacg ctcatttgtg aaggcgaaca gcgcgcgcag ctgtttgcgt
    11461 gctttcgcca tttccgcagc gtccggtggt gaggccgcga tcgcttccag ctcccgcaga
    11521 atcgctccct ccacctcgcc gtgagtcctg ccgtctctca caacggccgt gatgtcaaaa
    11581 aggaacgggt cgatacttgc ttccaggttg cagtcgacat cgattgccag ttccttttcg
    11641 accaacgcgc ggtacaagcg ggaactgcga ctctcagctc cgctaccgaa gccgagggag
    11701 atcgggcttc caaggatgga gccgagaaca agcgacggca caaaacgggg atcagttgct
    11761 tttggcgcaa gaaacgccat ttcgaggtag cccgtcgcac ccgggccctc aagcagaacg
    11821 cggcgctccg cgaccggggc tggttcaatg gcgcgaggtg caggaggcgt cgcaccgggg
    11881 ggaatttccg agaaaagctc ttcgagacgc cctaggagaa cccgcgtttc gaaatctccg
    11941 actacaacac acacggcgtt gttcggaatg taatacgtcc gaaaatacgc gagcaggtcc
    12001 tcgcgcgaga gatttcggat atcctcggcg atgccgatca cctcgtgccc gtaggggtgc
    12061 gcgtgaaatg cagcagccgt aacctgctcg gcaagtcgcc attccggttg gttttcatac
    12121 gcatcgcgct ccgcgagtat gacagagcgt tccgtttcaa aaacatccgg agcgaacaac
    12181 ggacctgaaa gacggtctgc ctcgatctgc aggacaatgt ccaacttgga ggacgggatc
    12241 gtcgtgtagt atgcggtgca gtctatccat gtgaaggcat tggtggttcc tccttcgcgt
    12301 gcgatcaagc gatcacgctc aatacccggg aatcgctggg aaggtttgaa cagcatatgc
    12361 tcgatccaat gagatacccc ggtctgcccg gggagctcat tccggctccc cacgcggtac
    12421 cccatccaga aactggttac cggtgcatga tgccgctcct ccgttaccac ggtcagaccg
    12481 ttcgtcagca cagacacttg atacggcggg ttcgccattc ggttctccgt tccgcgtcag
    12541 ggacgaacgt gagggccgaa cacgcggcgc agataaatac gattgcacgc gcgtccctga
    12601 tcgaaccgca ggtccacgat gtcgaaatcc tcggtgatac agagggtcaa catcccgcga
    12661 taccgattca gcgtattgat gtcaacggag cggtaccctc gcctgcgtat ctcctccagg
    12721 gtgaagcgga aaagccccct cccgatgcct ttgccgcgga tttcgggaag gcctcccatg
    12781 agagcgatga agaagacgcc gggctcctgt gcgtcatgac ccagcacgta cgtgacgatt
    12841 ctgccgtcca gcctcgctac ggtcagcaag gctcccggtt tttccgtgag agtctgcctc
    12901 cacgaggcag cgcccttttg caggtccttc gcgccgtccc attcgtaaaa tgcggcttcg
    12961 tggaccttca ccgcctcatc aaccgcctcg acacccgcta tctcgtatac cggttcgctc
    13021 acgtctgttt cccccgtccg ttgtgtggat aacgacttga gaaaaaatac gctaacccac
    13081 ctgcgcccca aagggcggcc tgtgcttgac atgcgtagga tggatggtgc actcttgata
    13141 acgtgggtat agatacttcc ccgattagtc gggggttcgc agaacgatgg acaagggaca
    13201 agagcagctc atgtgcgata acacggatat catggagtcg gtgaaggaaa tagccgagga
    13261 ggctggccgg atcgcgcttt cgcaattcgg cgccagccgg cctcacgtga aaactgatcg
    13321 gagcttcgtc actgcaacgg accgggaagt ggagcagttc atacaccgga aacttcacga
    13381 gctcttcccg gacgacgcca tcatcgggga ggaattcggt gggtcccctg gcaccatacg
    13441 ggaatacgtc tggttactgg atcccataga cggaaccacc aactttgttg cgggcttgcc
    13501 ccattgggca gtctgtgttg cacggctgcg gtttggcgtt cccgtgctgg gagtggtcgt
    13561 cctccccgtc ctgcgcgaga cctacgccgc tgcaacggga atcggcgcaa cgttgaacgg
    13621 ctccccgttc aaaatgctcg aacgtgacga tcctccaaac gaacaactga tagccgtatg
    13681 gtcaacgtgg taccgggaag tggacctcaa gttttcgggg aaggtccgaa ctctcgggag
    13741 tacggtcgtc aagtgccttt acgccgccaa aggcagtttc gttggcgctt tgacaccgga
    13801 ggttcacgca tgggatatcg ccgccgggct tcccgtcctc tgggaatccg gtggtcaggc
    13861 gtacgcgagg gatggtaagg tctacgaggt gatggatctc gatcctgcga acggatacct
    13921 cgttccgccg ctcattctca gcgccccttc gcggagggat tttatgcggc agatgatcac
    13981 tgccaggcga acgtgatgca tcgggtggga cgataggaat gaggctctcg cgcagcagca
    14041 ggatcccgct gatcgtcttc gtggcggttc acagtttcgc cgggtgtgcg tactataaca
    14101 cgttctacaa tgcggaacgt gcgttcgaaa aaggtgaacg cgcccgacaa agccttgagg
    14161 cactgcagcg gaccgggtcc cccgcgaccc ggcgcccttc ccagcaatca tcgtcgacga
    14221 gaggggcggc agtcggcgat gagcaatcgt accgcaccgc aatcgaaaaa tcggcaaagg
    14281 tgcttgtcta tcatccgcga agtcggtatg tagacgacgc gctgctgctc atggcgaaat
    14341 cgtattaccg tctgggggag tacgcacctt cgctgcgaaa gtgcaccgaa ttgttgacca
    14401 cgttcccgca gagtccgctg gccccggagg cgcggtactg ggagggaatg gctctgtggc
    14461 agcttggaag atacgaagag gccacgggtg tgctctcccg catcgccgcc gatgacggat
    14521 ctcggtttca gggtgacgca gcatttgctc tggcgaagct gaaaagggag cgggggtctt
    14581 cggaggaagc gattccgctg taccgcgtcg cagtacgtcg atccaccgaa cgggaattcc
    14641 gctaccgggc caggattgaa ctggcggaat gcctgattga ggcggggggg atgtcggaag
    14701 ctgtggaggt gtaccgcgaa ctgagccgcc tcccgcagac tgcagcagaa cgatatgaga
    14761 acctgcttcg gattgcgaga acgcaacgtg ccatgggaaa tgcagaagcc gcgctcgcaa
    14821 ccctcgaacc gctgattcgg gatcagcgtt tcaccgatca ggtgccgcgt acgagactgg
    14881 agatcgcaag gacgaaagaa gcggcgggcg aaatcgaagc tgctgttctt ctgtaccaga
    14941 aagtcctcga agatgcggaa cgtctcagtc ctccgacagt aactcaagcc ggtccgcccg
    15001 gcagcggacc ggccggcggg gcggcaccga cgggtgtgag aacgcgggaa acggcggagg
    15061 caaatttcca tctggggcgt ttgcaggagc agagattccg caatttcgcg aacgctgcaa
    15121 ggcactatgc ccttgcggcg cagagtccgg atcaggaagt tgccctgccc gccaaacggc
    15181 gaatggacga catatctctc tggacgcagt tacacgcaac acttgctgat accactgatt
    15241 ctgtgagcgt ctccaggaaa gaccgggcga ggtactccct tgcggaacat cacctgctgg
    15301 cgatggacga accggattcc gcgctgtttt acttcgacgc ggttgccgag cgaacgagtg
    15361 attcggatct ccggatgcgc gcgcagtacg cctccgggtg gatctggcgg gaggtgaaac
    15421 gggataccgc ccgttcagat tcgatctggc agaaattgat taccgattcg tcgcggacga
    15481 gaagcacgat ggaaatcaaa agggccattc actcagttca gtgccgcccc gagcaggaac
    15541 ctgggatcga cctgtatcag gaagccgaag ggcgctggtt cgattccctg gtccgttgcc
    15601 cgccgtaccc gccggattcg gtaacgaaag attctctgtc ctccgcaaca tggtgggatg
    15661 agtggcgagc gtgcgagaaa cgagagtgtg aatcggcgat caaggacttc aatcgccttc
    15721 tggagctgta tccgcaggga aatctggcga ctcgcgcacg gttcatcctc ggttggtacg
    15781 cggaaaatgt ccttgaggac actgcttccg cgtaccgttg gtacgatgct gcgcgcgaag
    15841 attcggtgtt cgcgccggaa gtcgggcgtg ttgctgacct ccttttgaaa tcacgcgtaa
    15901 ccggtggaac ggggcgttca gcagagaccg caggaattcg agatgcggca gccgctgaca
    15961 caacagccga actcggacaa cccttggagc cgcgtacacc ccgggaaccg gggccagaag
    16021 ctcctccaca accggaggag cgttctctgg agcggccgga acgagtggaa cgcgtgccgg
    16081 ttccacggag ggagccccgc cgccatgctg aatagcccat tgcctgcaac cgtgcctgtg
    16141 gacgaagctg cgcgcgtaac cgaacaggca gccgtcgctc ccggcttttt ccggttgcgt
    16201 ttccacgcac cgcacattgc gagacacgcc gttccagggc agttcgttca ggttcgagta
    16261 acggatgacc tcgagccctt tctccgccgt ccgttcagta ttggggacgc tgacccctca
    16321 cgcggcttga tcgaaatcgt gtatcacgtt gtgggaaagg ggagtaggcg actctccgaa
    16381 cggaaggcgg gcgaaacgat cccgctgctt ggtccgctgg ggaatggatt caggctgccg
    16441 ggctgcggca ccgttgttct ggtgggcggc ggcgttggca tgccgccggt atatttcgct
    16501 gcgaagtcaa tccgcggatg ccacgtgtgc gtcattcaag gggcgcggtg cgcggagcga
    16561 ttgctgtacc gggaggaact ctcgtctctc ggtatcccgc accgcgagac aacggaggat
    16621 gggtcggcag ggtcacaggg ccttgtcacc gcagcgttga tcgaaacgct ctccgatttt
    16681 ccgcccgacg aacgtaaagg gcctgtcgag atactcgcgt gcggaccacc tccaatgctg
    16741 gccgcggtag ccgaaattgc gcgcgcgcac ggggtgaggt gccaggtatc ggtggaagaa
    16801 cgcatggcgt gcgggttcgg catctgcatg ggatgtgccg tgcagaagcg cacggcaggc
    16861 agcaaagaat acgcgcttgt gtgtgttgat ggtccggtgt tcgacgcaac agagatcgca
    16921 ttggctcctt cgtcatgaat cgaacaggtc ccgcgaaaaa gaagcctcgt gttccgattc
    16981 ctctcccggc agaaaagccc tccgaagccc tgtatcgcct ttgttcgagc acaggtgtag
    17041 cccggttcgt tgagtggaaa aggcgcaatg ccggcgtgtt gtttttgagg atccgcctct
    17101 gtccatcaat tcggaatgtg gagccagacc gatgggatta cgactctctc cgggatgctc
    17161 tacgtctcat aggacgctgg taccgtttcg tctcaatgga cgaggcagtg tcggcactag
    17221 caggcaggtc cccactcttc aaaagcagca ttgcggttac cgtttcgatc gggagcagcc
    17281 tgccggaaat agatctgggg cggctcgcca ttgaatgtga cgtacctctc accgtgtttt
    17341 tctgtcccgg cgcagtggaa gcgggcgtcc acccatggta ctgtgtgctg gcgttcgccc
    17401 tgaatgggct tgacggccag acggttacgg tcggtggcag gaggtggctt ctttccaact
    17461 acgattaccg acagctcgcc ttcgcggaca tcgcgcgaca gattgcgagt ttgccgttga
    17521 cggaccgcta ccggtacacg gaagagctgg tttccggctg gcgcgcaaag cccgcaggta
    17581 gcgaggtaat tacgtttcgt gagatgatga gaatgatgcg tagcaaaaga attaccttcg
    17641 gggtttcggg gtattctgga gattcgctta tgcgtctgcc tgtagaacgc gcgctgtacg
    17701 agatacgcga agggtttgct ctttaccgga gggttttcgg ggaggaacca gcccattttg
    17761 agtacccatg gggagacaga agcgtctacc tggaggaatt cgtaaggaat tccgggtacc
    17821 gctcgggtac tctggggctt aatgccatgg acgggctgaa ccgtattgga gcggatccgt
    17881 tccggctggc ggcacaaaat ctgaggccgc gaacgcctgc tcgcatacgt gcggacctcg
    17941 cgggggtgag caagcagtta cgcccgttgg aaagattcct cgacgttcga acgcgaagag
    18001 cggatcgcga cttctgaaca atcctggtca ttgatgatgg aaacgctggt gttcccagca
    18061 gcgttcgtgc aacaaccgat tacgtttccg cggccacggt ctgcttctca atggagcccc
    18121 gctgcggtcg aacatctccc tccttgtgat tgcgaccaga gccgattgct cctcgttcag
    18181 gtcaaacagc ttctgaagta cgagagcggg agaggccgtg tgcccctctt cgtgcgcgat
    18241 cgccagccga agatagacgg ttgtgatgct ggcggaactt tcggaccgcc agttggtaac
    18301 agctttctta agatcgcttt cccggacgtg ccctttggga ctgacccttt ctgcgagcca
    18361 ttctccttgc tcatcgaaca gctcgcgcaa agccttcacg tcgcactcgc gaagcaggga
    18421 gtgtgggatc tcgaacgagt attccgcgta gaacgcgatc cggtccacag gttcggcttt
    18481 cctgtctatc cggtcgcact ggacacactg caggccgtca gcgagacggg aattgattcg
    18541 cgataacacg tcgtccagag tgctgtcgag ttctgcgtcg cagtattcct gcatgctgga
    18601 acaaccggcg ggaagcggcg ggccgaaccg cagttttggc gtggttccgt cggggtttcg
    18661 tgcaagcagg ccggcatcgg cgagcgccga ctgtatgagt ggcatgatct cccgatattc
    18721 gagagctgcg gccgcgtttc cgacggagta atggagtcgg caaaacgccg cggttccgcg
    18781 cacccgcggc gtgcagccgt tgaggagtcg ggtgggttcc agttcgaagt gtccttcggg
    18841 gcgcgtcgct cggtggtcaa cgttgttcgc gcaggctcat ttcccgctgt ttcgcggccc
    18901 ttcgcacgtg cacgatgcgt tcgcatgtgg tgaggttcgt gagtatcgcc attacccata
    18961 ccgcggcggc aacgtgctga tagggagtat tgagaaacac gccgaaaacc gatgcggcgg
    19021 cgagaaaaat cacacgttca ggtctctgca tcaaaccgac tttgcattcg tagttcagcc
    19081 cttccgcgcg cgcccgaacg tagctgacga gaagagaacc ggtcagcgcc gccgcgctca
    19141 gcaccgaggt tatcatccag ccgattcgtg cgtaatggaa ggacaacgcg attaggacca
    19201 cggcctcgct gtatctgtcg agaacagaat cgagcaacgc tccaccgtcg gatactccgc
    19261 cagttttgcg ggcaatctgc ccgtcaatca tgtccgtgga gctaccgaac agcatcacaa
    19321 gagcgccttc gaagaacatc cctaaggcat agaacccggc cgcgatcgtg ttgaacacca
    19381 gacccatgat tgtgaaggta tccgggtgat acccccgacg aatgaaatgg ttcacgaggg
    19441 gatcgacgaa aacttgaaac ccgagcttca ggtcactgaa catgttctga tccgcattcc
    19501 gcacggggcg tgcgtgtctc cacactctcc gtgcccgaca ccaccagtac aaactcgccg
    19561 cgcaacacct tatcgtcgca ggatgaaaga agcgttgaaa gagtacctcg ccgcgtctct
    19621 tcgaatttct tggtcaattc cctcgacaat gacgctttcc tgtctccaag cacttcaagg
    19681 gcatcgcgaa gagtgctgcg gacggaatgg ggcgtctcgt aaaagatcat cgtccgcggt
    19741 tccgccgcca gctcctcgag ttttcggcgc cgcgcactcg atttcttggg aagataccct
    19801 tcaaacgcga aacggtggac gggaagacct gagactacca gtgcgaggac gaatgcggtt
    19861 ggcccgggta ccgggatgat ttcaattccc cgttgaatgg ctgcctgaat gatatgccat
    19921 cccgggtccg cgataaccgg catccccgca tcgctgacca cggcgacgtc cttaccttct
    19981 tccagcgccg aaaggagttc tgggaatttg gttcgttcgt tgtggtcgtg atagctcgtg
    20041 agagggcacg aaacgcccag ttctttgagc agaatacgcg ttcggcgggt gtcttccgca
    20101 gcgataaggg aaacgctcgc aagaaccgcg cgaacccggt cactcacatc cccgaggttc
    20161 ccgatcggtg ttcccacaag gtaaagccga ccggtcataa ccggatcacc ttctcacagc
    20221 gaagaataag cagaaaaaac gcccccgcgc actctgaaaa agatactcat ccggcgcgta
    20281 cgctggcaac ccgattactc tcgagggctc agaacgtggg cattttcggc aggacgtacc
    20341 gctatcctgg cattgaggag ggactcacgg tctgaccacg atgcgatttg ccacgtggaa
    20401 tcgctcttcg cggtatcgag tctgaatgcg atgtcgaacc gctgacgaac ctggtagccc
    20461 gtctgaactg cgttgttatc agtgttggtg aaaatcaggt ccccgagaac gcgcaatacg
    20521 acccagtttt cagtagcgtg cgcgctggaa atctggtaaa gcgcagttcc cggcgggtat
    20581 ggcatgtcgg ctccatactc gatccaacgg ttttcaattg cgaaatgcgc agatatgtag
    20641 tcaaagcttc tgaaaaggtt tcggtagacc tgtatttcgc tcgcccggtc gagttcgaga
    20701 tcgtttttgt cggtcggatc gtcgtaccgg tacacgtacc cggcaaaaag gagatcagcg
    20761 tactcctgaa cagattccgg ggtgtgacga tcgaagat
//
LOCUS       DAOSOO010000032        20485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2605_length_20485_cov_5.665198, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000032 DAOSOO010000000
VERSION     DAOSOO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2605_length_20485_cov_5.665198"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLT86_03975"
     CDS             <1..401
                     /locus_tag="PLT86_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35807.1"
                     /translation="RPMPDPFPFSLRPAPKRGLVLTFILLAMMIVYGMTATGVLAYYL
                     APERAPQLPAALDPRLFPFVGLLALLKTAACIAVWRWQRWGVYVLLITAGAMVVADLS
                     LPQPVLGSFVDALGALLVLVLCRRTWEDFA"
     gene            398..1309
                     /locus_tag="PLT86_03980"
     CDS             398..1309
                     /locus_tag="PLT86_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT35808.1"
                     /translation="MTESPPLVSVVMPTHNSASHGFLRPAIESVLGQTWRNLELIIVD
                     DGSVDGTPDFVRQEYASDARIKLFELEHVNQANARNYGVAHSSGRYIAFLDSDDTWKP
                     EKLERCLLKFEEGPVPFSGLLFHPMDTIDETGTIRKTRAAQTRPLAYTELLEHNPIAC
                     SSVVVPKRILENAGPMRLDPPCAEDYDLWLRIAVKHPILCLGEVLGASRVHQGNVSRK
                     TDLMEQAELTVIGERLRLLTNREADRIRARHLSSFARMRFGNNDFAGFQKHYATLRSL
                     GRVDWPLRIRYALSLMPCILRMIRRAP"
     gene            1563..2087
                     /locus_tag="PLT86_03985"
     CDS             1563..2087
                     /locus_tag="PLT86_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HOT35809.1"
                     /translation="MDVGSRIDGFIAHLAAFREVEVFDIRPLAVPIPGVTFRQVDLMA
                     PGADFSGLEGYCDSLSCLHALEHFGLGRYGDEIDPDGFEHGFANMARMLSDGGTFYLS
                     VPIGINRIEFNAHRVSDPRMIVDMAERNGLLLSSLTTVKDGREVKRATREDLADLARA
                     RYVLGVFVLVKKQS"
     gene            2095..2403
                     /locus_tag="PLT86_03990"
     CDS             2095..2403
                     /locus_tag="PLT86_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35810.1"
                     /translation="MVIVQLTGGLGTQLFQYAAARAIAHRNGVPLKLDTSWFKRYPQR
                     AFALGGFMIEASVATTEEIEELCPPRWSLRWAGGQARARTKPYSRLAQACGQGTVETV
                     "
     gene            2375..2983
                     /locus_tag="PLT86_03995"
     CDS             2375..2983
                     /locus_tag="PLT86_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HOT35811.1"
                     /translation="MVRERWKRFDPALLKVGGDAYLIGYWQSERYFMDIAELIRRELV
                     FKTPPDERNAELLSRIDAIDSVSLHIRRGDYVTNPVLADIYGTPGLQYYARALEVVAG
                     HTDAPHVFVFSDDIPWARENLVTNLPTDFVCHNGPGKEIEDLRLMSRCRHHIIANSSF
                     SWWGAWLAEHPGQLVIAPDPWFRDRKWAPADIVPERWTTLPA"
     gene            3223..4518
                     /locus_tag="PLT86_04000"
     CDS             3223..4518
                     /locus_tag="PLT86_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT35812.1"
                     /translation="MNGTYETLRADIATIPVIDCHEHTNGPASGPTYSDPLAALVVGY
                     VNSDLQSAGTEPVLAAAMDTSKPLEERWKLFEDIWKRTQHTSYARVTKWVLREMYGEK
                     ELSLDALKRMAGNLLNLKDEKTYWGILDKAGIKCRIVNIWVGDRKIIEREHVLPDRDR
                     YMIGLPGYHSIHSWNEVYSHTQQIGRTAASLDEYIDICREIFTRLKGRGAIGMKDQSA
                     YNRTLRFDNATREAAEKVFNFFMEDPRRSVGYPESKPLDDYLFHAFMRIARDLDMPVQ
                     IHTGHMAGVRNDIAKTNAVHFTSVLELHREVRFDLFHGNWPYMGEWLYLGKNFPNVAL
                     DCCWLHIIDSIYAQNVLAESVAAVPHGKIHGFGGDYGDSVEFAAGHLSIARDNIAAAL
                     ARLVDSGWIGMDDARQIAADWLFNNPNRFFRLGFETVTV"
     assembly_gap    4697..4796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4797..5750
                     /locus_tag="PLT86_04005"
     CDS             <4797..5750
                     /locus_tag="PLT86_04005"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT35813.1"
                     /translation="RPTRGVVLSLSDITSWNWPEEASAAGLTTIATHGRAGDSAEFMQ
                     SEVGEELRTRCERASVEIEHELHAARELLPRELFARHPDMFRMNEAGDRVPDANLCVH
                     SEEALEVCCQNASALARALAPTTGRHFFWIDDGQPMCRCPRCRHYSDSDQALILENRL
                     LTTLRKDNPKATLAHLAYANTLWPPADVKPLPGVFLEFAPIQRRYDLPFGVRDAQSGG
                     EKAPGHGEQLDALDANLELFGAEGAQALEYWLDVSRFSGWNRANVARIPWDKRIVEAD
                     AKLYHDRGVRNVTTFAVWLDGDYVRRFGTPPLREYGDALRW"
     gene            5744..>6222
                     /locus_tag="PLT86_04010"
     CDS             5744..>6222
                     /locus_tag="PLT86_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35814.1"
                     /translation="MVEIPAKTVWIDRRIADHPECRRRLEWVLPHVHCRDIRYCDGNP
                     GEEFFSVSKRRHGKDDFSDDAVLAFTTYDSGRRGFYFHWRDEAAAHGGACQPAMELNI
                     VEGCVFRCAYCGFGRRVHFTLDVERLIGELEAVFAKHPEQRLYKFSNMTDLPAFEPE"
     assembly_gap    6223..6322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6323..6980
                     /locus_tag="PLT86_04015"
     CDS             <6323..6980
                     /locus_tag="PLT86_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35815.1"
                     /translation="DRYLMLFTKSDNVAFLRDLDHRGHTIISWLLTADSASRLLDKRT
                     ATMDERITAMAEMQKAGYLVRARLSPIIPVRNWEKEYRALFEHMFAHVQPDLVTLELL
                     GWMKFDDLLAVIPPELLDGQMREEARAAADSLKDVMWAPFTQDAHEKVYRFCIDTVRE
                     LSPRTPVSVCHGTAATWKALGELTGMTPGEYICNCGPLSAPGGALYDELHALHDGEGR
                     "
     gene            6977..7723
                     /locus_tag="PLT86_04020"
     CDS             6977..7723
                     /locus_tag="PLT86_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT35816.1"
                     /translation="MNERFVQLDRGDGTRVTARVRAGKGPNLILVPGTWGNMDTRGPL
                     MERLDPELNIVNVALPGQDDNWPPPKDPSIPAFTADVLRLADHLGLERFFVGGNSLGG
                     MISIDMIRLAEQRLLGVITLEGWTHWSALYDAFGDNTEVTLNPQQSAFLQEVRRKLLD
                     RWDPGMRSRYATMWREWDGSETLQNCTVPLLSIWGDRGKPRPAPDKLKVPVKPNIEFL
                     WVANASHDLLVEAPEIIADAMNEFIRRHNA"
     gene            7876..10185
                     /locus_tag="PLT86_04025"
     CDS             7876..10185
                     /locus_tag="PLT86_04025"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOT35817.1"
                     /translation="MTSSVEGVSMPLFPTDLPEREWCQFNAEGFSRPVCGLIHWGTNA
                     PVCGMPLGGIDTGCLDLEATGVWGYCSIFNSLNPRRGAINEPFLGISIGRQTWVLSTV
                     ALSRRDGQEWIDTMRSWANYRAVKSASDIHYWGHFPVADLEYETDAPLSIGMRAWAPF
                     IPGDVALTNAPAAVFEVHLRNSTETEQKGTLAFSFPGPNEGEAGTTTFRRVKIEGAAT
                     GVSVESPQASYVLTAIGESSVRTGGCLGTDAVAWSCIEDALPFVEAGAGTSIAVDFAL
                     SPGAEKTIRFVLAWFAPVWNGGGIMTGKGQDYRHMYARRYANARAVAEMITGRHEEIL
                     RRIIAWQSVLYGDEGYPVWLRDALVNVLHLIPETSIWAQAKPPVGDWCNEEDGVFGMN
                     ESPRACPQIECIPCSFYGNFPLVYFFPEAALSTLRAYKAYQYPSGQAPWVFGGCTVGS
                     PPYEMAMPSPGYAKKPQTTLDGGCYVDMVDRMWRRTGDDQLLREFYDSVKRNTIFTMN
                     LRPGSGAAGIVSMPEGNQAQDWFESCDLFGIVPHIGGVHLAQIRMARRMAKAVGDEEF
                     VRQCDSWLAQGSEVLENETWAGTHYLLFKEKETGKESDIIMSYVFDGEWMAKLHGLEG
                     VFRPDRVKTMLETIRRTAIAMSPFGSVVFCKPEGALGKEDWDPGYWGAHGVHPPGTFM
                     LAMTYLFYGEKDTGLDLARRTMGEIVKRGWYWDVPVAIDGTAPRIGFDYYQNLVLWSF
                     PAVMEGADIAAPCKEGGLVDRVIKAGAEA"
     gene            10309..11589
                     /locus_tag="PLT86_04030"
     CDS             10309..11589
                     /locus_tag="PLT86_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase-like family protein"
                     /protein_id="HOT35818.1"
                     /translation="MATAGTPVHAAATLPVHLPAKLSICCWIWSWITSATEGEPYHDL
                     DRCMAGLKERGFNAVRVEAGLNWAFRADGSPRGQVAFRPWIAGYGWNFSTVNSRGGGR
                     HDVLQRLVALFESARRHGVYVILTSWEYQDSTSFLADPALRAEIHAVPPGNRYMHLAK
                     LHDRLLAVLEERGLEQNVAFVEVHNEPDASEFPGGEEGRELHEEAIGFLRDRHPEVLI
                     TGDFTSHDYAIVPENVQVFDQHVYAGFGWYFRTLFGETVLNKDFDPGKPRALPALNRV
                     LRRDLVPWSEFMVAAENVREFWRPIMWMYENLDNEKWDEWVAETFTEWAPRIREKAET
                     AFASDAREGRRRGLPLVFDEGGYFYPPRLARFEVFGEGLSLFELMCDLAITHGYWGFM
                     PGTYCGPEHLAWQENPAWLLGINTRFQKGILSGS"
     gene            complement(11650..12960)
                     /locus_tag="PLT86_04035"
     CDS             complement(11650..12960)
                     /locus_tag="PLT86_04035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT35819.1"
                     /translation="MNTNPFHSLGLDDRILRALVDQSYETPTPVQLDSIPRVLEGRDL
                     LATAQTGTGKTAAFCLPMLHLLNTREGRSNKHVRALILTPTRELALQIGESLRAYGRY
                     LPLRFTVLLGGVAQAPQAKALREIPDIVVATPGRLLDLFGQRLIQLDRLEILVLDEAD
                     RMLDMGFVRDVRKIVSATPATRQTLFFSATLSPEIAELASDMVKNPAMVAVTPVASVS
                     DHIAQKVLFVEQTDKLSLLTEILREDSVRRALVFTRTKHGADRIVRQLERKGIRAEAI
                     HSNKSQNARQTALAAFDRGRTNVLVATDIVARGIDVDGITHVINFDLPNEPEAYVHRI
                     GRTARAGAVGTALSFCGEDEVSLLFAIEKLTTCPLSDVDGHAFHSTAISAMRDRKRAA
                     QQGTGGSSRFGSGRSGRNRPLSSQRRRYHPTAGPATGARVSHAA"
     gene            13396..16638
                     /locus_tag="PLT86_04040"
     CDS             13396..16638
                     /locus_tag="PLT86_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35820.1"
                     /translation="MHRMTIPWITTAQTTRGGRGGTTTAERTIALKCDLPDEPAVRVT
                     GELTLARPEREATLSVGSSVCTLGVSGDASDPYLLHARAWVSDPDSPRGTVAYRLVPD
                     VTRWAHDEAGREQYRREFAHAKGWAEHRIRLSIDVTPCEVRAWVDGRLVASWSARNPQ
                     PGVALVHLPEGARPHGAAEVRILPGEEEFLPLDLHPFARGEDFLNLKETAYRGRAPRV
                     FASSLSSDPKRALFRIPRKTYDRVHLVCSASTEGGALPSASVAFIKPERGHTVFTEFL
                     VNPPYHTPKTITVPLDPCAFSDFLNDPGEEALEVEFCQRMDYDEGRFPHPAGPPSGVR
                     IHEITFETAPVALRVTSSAVGHVFQGTKTPRFFVHLESQRSVPGTVRVAVEVAEPYGG
                     KSKSSARVRLAPWEKRTQTFELPQRMYGRFGLTVTVSQEDGSRPLKRSTTFALLPRDT
                     RKATSDSPFGIWCFFENHHGVPPEIAAPLFRKAGARWTLANFVVRDDPDETRKRVQTL
                     AAHGVGLACANVACIGNTCNAGGNDVDAMIRRMHAMPPVKHWLLFWETFLSRQHMRDY
                     PFDILGRPVRELTPDEESVFKNCWDTAIEYATRVRKEFPEAKLIFGNGHPNFIAAFLR
                     RGFPKEYIDGFGLDFDMFVSSPELQPGPLHCPFSGLFFLKTLQDLYGYSEIPRYLTEA
                     IYCSQSEGWLTERQQADHYVRSHLLGLASGVVLFGMTAELNDPGSEYFYGSYGPVGFL
                     RRAPELNPRESFCAYATMTRMLDRANFTEMVPMPSPGTYGLRFTKADGSDIYAFWTTT
                     GTRLLTFGAESGSVRTTDLFGNQTVIRNARGVFSLNVSPSPVYVEGEGIHGFVLGTPR
                     HRPGPKTSEPIVRMGDLPSWRHEAEDDAWMERLGRAGNWAITAPTIRGRFEFSLRKDR
                     ARGQVLRVSLSETPGAHPLHLRYAVLRFSRKVPVNGAFSALGLWIRGNSSSGRVLYEL
                     EDADGIVWRSVYSDTYIDFDGWRYVQTPLPLPATEDGSRLQPCGLTPWATETEHRHPA
                     LPVRLTALIIESRTHVVRATSLDPVPDTTFDVSDVVVTKEMVDPEIRFRW"
     gene            16707..17684
                     /gene="miaA"
                     /locus_tag="PLT86_04045"
     CDS             16707..17684
                     /gene="miaA"
                     /locus_tag="PLT86_04045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT35821.1"
                     /translation="MCTGSSHPAEDNALPVIPIILGPTGSGKTALVVDLVRDGRLTGI
                     EVVSADSRQVYRFLDIGTAKPTEEERHTVPHNLVDILDPSETYSAGRFRRDAEAIIAE
                     ILKRGSTPVVVGGTGLYLKALVDGLSPIPRVPVDVVDRLRAEIASVGPPALHERLQEL
                     DSRCAATIQPTDTQRIIRALAVHEATGKPLSRWWEEHPEKPPYRYVWLGIRWDRSQLR
                     DRIARRTRAMIARGLEREVRWLLDRGYTWEMNAMATVGYREWKHCFAGEITREEVTAA
                     IAIHTAQYAKRQMTWFRTNPRITWVDGSSPSLKDEVVRWLDDRHAEAKR"
     gene            complement(17733..18164)
                     /locus_tag="PLT86_04050"
     CDS             complement(17733..18164)
                     /locus_tag="PLT86_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT35822.1"
                     /translation="MAMVPTLPSIQNEINRLFDALSSRFGEFSGETLSDWAPRVDVIE
                     DADNLRIYADLPGLNREDIQLSVERNTLTITGVRKPMEDANKFSWHRAERLYGTYKRV
                     FNLPTVVDPDKVSAEYRNGVLEITLPKAEHAKPKMITVKTE"
     gene            18513..20396
                     /gene="ftsH"
                     /locus_tag="PLT86_04055"
     CDS             18513..20396
                     /gene="ftsH"
                     /locus_tag="PLT86_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT35823.1"
                     /translation="MAFERRTQLSLWYFVGAFMILLALNLYFSKSQVEPLNYSVFRQY
                     LKQGRIVEATLTAQHITGAMRTGTSTSTLARFVTVRVTDPELLDLLTQHGVIFRGRVE
                     DTWVTEMLAWIGGMAVFFVIWMFLIRRMGGPSSGMMSIGKSKAKIYVETETKVTFADV
                     AGIDEAEEEVREVVEFLKEPERFRKLGGHIPKGVLLVGPPGTGKTLLARAVAGESGVP
                     FFSLSGSDFVEMFVGVGAARVRDLFQQAKERAPCIVFIDELDALGKARGMNPLGSHDE
                     REQTLNQLLVEMDGFDANVSIIIMAATNRPEILDMALLRPGRFDRQIVVDRPDLNGRL
                     EILKIHAKRVKLDPEVDLRQLAAGTPGMVGADLANVVNEAALLAARRGHDAISQNDLQ
                     EAVERTLVGLEKKSRVLNERERTIVAYHEAGHALVGLCSPHATPVHRVTIIPRGVAAL
                     GYTMHLPTEDRYIQTKAELDAELGVVLGGRAAEEVVFNEISTGASNDFVQATRIASSM
                     VKDYGMSRLGVVSYDDENQSRFLRQISGGMPQYGERTAAQIDREVRAIIDERYQHVKK
                     LLSEKREQLEAVAQRLLATESISSEELRELVEGCGANKPAQEVPDSVQGEGVTSDATK
                     EDA"
BASE COUNT         4106 a         5829 c         6229 g         4121 t
ORIGIN      
        1 ccagacccat gccggacccg tttcctttct cgttgcgtcc cgcgccgaaa cgcgggcttg
       61 tgctcacctt cattcttctg gcgatgatga tcgtgtacgg aatgaccgcg accggcgtac
      121 tggcgtacta tctggctccg gaaagggcgc cgcaactgcc tgctgcgctg gatccgcgtc
      181 tttttccttt tgtgggtctg cttgcgcttc tcaaaacggc agcctgcatt gccgtgtggc
      241 ggtggcaacg ctggggtgtg tatgtgctcc tgataaccgc cggagcgatg gtggtggctg
      301 acctgtctct tccgcagccg gtgctcgggt cgtttgtgga tgccctcggc gccttgctgg
      361 tgcttgtcct gtgtcggcga acctgggagg atttcgcgtg acggaatcac ccccccttgt
      421 ctctgttgtg atgccgacgc acaattcggc ctctcacggg tttctgcggc cggcgatcga
      481 aagcgttctc gggcagacgt ggagaaacct ggaactgatc atcgtcgacg acgggtccgt
      541 tgacggcacg cccgatttcg tcaggcagga atacgcgtct gacgcaagaa tcaagctgtt
      601 cgagctggaa cacgtgaacc aggcgaatgc gcgcaactac ggggtcgcgc actcctcggg
      661 gcggtacatc gcgttcctgg attccgacga cacgtggaaa ccggaaaaac tcgaacggtg
      721 cctgctcaag ttcgaagaag gtcccgtccc tttttccggg ctgcttttcc atcccatgga
      781 taccattgac gaaacgggaa ccattcggaa aacgcgggcc gctcagacca ggccgcttgc
      841 atacacggaa ttgctggaac acaatccgat agcctgttca tctgtggtcg tcccgaagcg
      901 aatcctggag aacgcaggac cgatgcgatt ggatccccct tgcgcggagg attacgatct
      961 gtggctccga atcgccgtga agcatccgat cctgtgtctt ggtgaggttc ttggagcctc
     1021 acgggtacat cagggaaatg tctcgcgtaa gacagatctg atggagcagg cggagttgac
     1081 ggtaattggt gagcgactgc gccttttgac gaatcgcgaa gcggatcgca tccgtgcaag
     1141 gcatttgtct tcttttgcgc ggatgcggtt cggaaacaat gactttgccg gttttcagaa
     1201 gcattacgca acactgaggt cgctcggtcg cgtggattgg ccgctcagga tacggtacgc
     1261 gttgtcattg atgccttgta ttctgaggat gatacggagg gcaccgtgat cacagtccgg
     1321 cgcctgcact ggctttcgat tacgcaactg gggtttgacc ccaaagtcac tttccggtcc
     1381 ctgaaagcct tgcctcggta tgtgaaagga tattggacgc tgaggggcaa gtacgagggg
     1441 ccggtggagt tcaccccgtg cctgcaggac tggttcaaac aggcgggcga gacggaaagc
     1501 gactatttct ggcaagacct tattgttgcg cgaatgattt tcgagaaaaa ccaaggaagc
     1561 atgtggacgt cgggtcccgt attgacggat tcatcgcgca tttggcggcc ttccgcgagg
     1621 tcgaggtgtt cgacatacgt ccgcttgcgg tgccaattcc gggggtaacc ttccggcaag
     1681 ttgacctcat ggcgcccggt gcggacttca gcggacttga ggggtactgc gattcgctct
     1741 cctgcctcca tgcgctggaa catttcggcc tcggccgcta tggcgacgaa atcgatccgg
     1801 acggcttcga acacggcttt gcaaacatgg cgcgcatgtt atccgacgga ggcacttttt
     1861 acctttcagt tcccatcgga atcaatcgga tcgaattcaa cgcgcatcgc gtgtcggacc
     1921 cgcgaatgat cgtcgatatg gccgaacgga acggacttct gctttcctcg ctcacaaccg
     1981 tgaaagatgg cagagaagtg aaaagagcca cgcgtgagga cctagctgac ctggcacgcg
     2041 cgcggtacgt gctcggagtg ttcgtgctgg tcaaaaaaca atcctgaggc cgcgatggtt
     2101 attgttcagc tcacgggagg gctgggaact cagctttttc agtacgccgc cgcccgggca
     2161 atagcacaca gaaacggcgt tccactgaaa ctggacacct cctggttcaa acgttacccg
     2221 caacgcgcat tcgcgcttgg cggcttcatg attgaagcca gtgtagccac gacggaggaa
     2281 atcgaggagc tatgtccgcc gcgctggagc ctccgctggg cggggggaca ggcgcgggcc
     2341 cgaaccaagc cctactcgcg actcgcgcag gcgtgtggtc agggaacggt ggaaacggtt
     2401 tgatccagca ctgctcaaag tgggagggga tgcgtatctg atcgggtact ggcagtccga
     2461 gcggtatttc atggatattg ctgaactgat ccgccgggag ttggtcttca aaaccccgcc
     2521 agacgagaga aacgcagaac tgctctcccg aatagacgca atcgactccg tgagcctgca
     2581 catacggcgt ggcgattacg tgacaaatcc cgttcttgca gatatctacg gcacacccgg
     2641 tctccagtat tacgcacgag ctctggaggt agttgccggg cacacggatg cgccccacgt
     2701 ctttgtgttc tctgatgata ttccgtgggc ccgggagaac cttgttacaa atctccccac
     2761 ggatttcgtc tgtcataacg gtcccgggaa ggaaatcgag gacttgcgcc tcatgagcag
     2821 gtgccgtcac cacattattg cgaacagctc gttcagttgg tggggcgcgt ggttggcgga
     2881 gcaccccggg cagttggtta ttgcgccaga cccgtggttt cgcgaccgca aatgggcgcc
     2941 cgcggacatt gtgccggaaa gatggacgac cttgccggcg tgaatggaga gagccgggta
     3001 ttccacggta cgaaccattg atatggcggc cagcctgcgt gtctgttggt gcgcttcctc
     3061 ccctgcttta ttatccactg caacaaactg gtgcgccgtg gctcacgacg cacaacgcag
     3121 aatcaatgtt gcggcgttga acacattgac ataagcggag ttatcgtttc ttccagtcgg
     3181 gaaactgata accagcctca tccatccgga aggagaaccc atatgaacgg cacatacgaa
     3241 acactccgcg cggacatcgc aaccattcct gtgattgact gccacgagca cacaaatgga
     3301 ccggcgagcg ggccgacgta cagcgatccg ctggccgcgc tggtcgtcgg gtacgtgaac
     3361 tccgaccttc aatctgcggg aacggaacct gttctggcag ccgccatgga cacttcgaag
     3421 ccgctggaag agcgatggaa gttgtttgaa gatatctgga aacgtacaca gcacacgtcg
     3481 tacgcccgcg ttacgaagtg ggtgctccgc gaaatgtacg gtgaaaagga gctttccctt
     3541 gatgcgctca agcgcatggc gggaaacctg ctcaacctga aggacgaaaa aacctactgg
     3601 ggcattctcg acaaagccgg tatcaaatgt cgcatcgtca atatctgggt cggagacagg
     3661 aagatcatcg aacgggagca cgtcctgccg gaccgcgacc ggtacatgat agggctgccc
     3721 ggatatcaca gcatccactc gtggaacgag gtgtacagcc acacccagca gatcggcagg
     3781 acggctgcct ctctcgacga gtatatcgac atctgccgcg agatattcac tcgtctcaaa
     3841 ggtcgcggcg ccatcgggat gaaggatcaa tccgcgtaca acaggacgct gcgattcgac
     3901 aacgccaccc gggaggcggc ggaaaaggtt ttcaacttct tcatggaaga cccgcgccgt
     3961 tctgtcgggt accccgaaag caaaccgctc gacgattacc ttttccacgc gttcatgcgt
     4021 atcgcgcgtg atctggatat gccggttcag atccacaccg gacacatggc aggcgtacgt
     4081 aacgacatag ccaaaacgaa cgcggtgcat ttcaccagtg tgctggaact gcaccgcgag
     4141 gtccgctttg atctgttcca cggcaactgg ccgtacatgg gcgagtggct ctacctgggc
     4201 aagaatttcc cgaacgtggc gctggattgc tgctggctcc atatcattga ctcgatctac
     4261 gcacagaacg tgcttgccga gtcggtggcg gcagtgccgc acggcaagat tcatggattc
     4321 gggggcgatt acggggattc cgtggagttt gcggcggggc acctttcgat cgcccgggat
     4381 aacattgccg cagccctggc tcgactggtt gattccggct ggatcggcat ggacgatgcc
     4441 cgacagatcg ccgccgactg gttgttcaat aacccgaacc gattcttccg cctcggtttt
     4501 gaaacggtaa cagtgtgaac cgcgggactc gcgcaggacc gggctgacgc ggggagtacc
     4561 gaaattgacc tttgtgcgcc gcggaacgca agaaggaagc cggatgcgat gacggaaacc
     4621 tcaccaacgg cggcgggaag gcgtcccacc cggggcgtgg tgctttcgct ttccgacatt
     4681 acgagctgga actggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgtc
     4801 ccacccgggg cgtggtgctt tcgctttccg acattacgag ctggaactgg ccggaagagg
     4861 cttccgcagc aggtcttacg acgattgcca cgcacgggag ggcgggggac agcgccgaat
     4921 tcatgcaatc ggaggtgggt gaagagctcc gaacacgctg tgaaagggcg agtgtcgaaa
     4981 tcgagcatga actccatgcg gcacgcgaac tcctcccgcg cgaacttttc gcccgtcacc
     5041 ccgatatgtt tcgcatgaat gaagcgggtg accgtgttcc ggatgccaac ctctgcgtcc
     5101 attcggaaga agcgctcgag gtgtgttgtc agaacgcgtc ggcactcgcc cgggcgcttg
     5161 ctccgacgac cggacgtcat ttcttctgga ttgacgacgg gcaacccatg tgtcgttgtc
     5221 cccgatgccg ccattattcg gacagtgacc aggcgctcat cctggaaaac cggttgctca
     5281 cgacactgcg gaaggacaat ccgaaggcga cgctggcgca tctggcttat gcgaacacgc
     5341 tctggcctcc ggcagacgtg aagccgcttc ctggcgtgtt tctggaattt gcacccatcc
     5401 agaggcgtta cgacctccca ttcggtgtgc gggatgcgca atctggcggg gagaaggcac
     5461 caggccacgg cgaacaactg gacgccctcg acgcaaacct tgaacttttt ggtgcggaag
     5521 gagcccaggc gcttgagtac tggcttgacg tttcgcgctt ttccgggtgg aatcgggcaa
     5581 atgtcgcacg aataccctgg gacaaacgaa ttgtggaagc ggacgcgaaa ctgtatcacg
     5641 acagaggcgt acgcaacgtt acgacgttcg ccgtgtggtt ggatggagat tacgtccgaa
     5701 gattcggcac gccgccgtta cgtgaatatg gagatgcgtt gaggtggtag aaatacccgc
     5761 gaaaactgtg tggattgatc gccgcatcgc cgaccacccg gagtgccggc gccggttgga
     5821 gtgggtgttg ccacacgttc attgtcggga tatccgctat tgcgatggaa atccgggcga
     5881 ggagtttttc tcagtctcga aacgccggca cggcaaagac gacttcagtg acgatgcggt
     5941 tctggcattc acgacgtacg actccggtcg acgcggcttc tatttccact ggcgcgacga
     6001 agcggcggcg cacggcggtg cgtgccagcc ggcaatggaa ctcaacatcg ttgaagggtg
     6061 cgtcttccgt tgcgcctatt gcggtttcgg tcgacgggtt catttcacgc ttgacgtgga
     6121 acggctgatt ggcgaactgg aagcagtttt tgcgaagcac ccggaacagc ggctttacaa
     6181 attcagcaat atgacggatc ttccggcctt cgaaccggaa atnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnagatcgct atctcatgct gtttacgaaa agcgacaatg
     6361 tggcgtttct gcgggatctc gaccaccgcg gccacacgat cataagctgg ttgcttaccg
     6421 cagattccgc aagcagactg ttggacaaac gtactgcgac catggacgaa cggatcaccg
     6481 cgatggcgga gatgcagaag gcgggatatc tcgtccgcgc acggttgagc cccatcatac
     6541 ctgtgcgaaa ctgggaaaag gagtaccgtg ctctttttga gcacatgttt gcacacgttc
     6601 aaccggatct ggtgacgctg gaattgctgg gttggatgaa gttcgatgat cttctcgccg
     6661 tgattccccc ggagcttctc gacgggcaaa tgcgtgagga agcccgtgca gccgctgata
     6721 gcctcaagga tgtgatgtgg gctccgttca cacaggatgc tcacgaaaaa gtgtaccggt
     6781 tctgcatcga tacggtcaga gagctgtccc cgcgcacgcc ggtaagcgtg tgccacggca
     6841 ccgccgccac gtggaaggca cttggcgaat tgacaggaat gacgcccggt gagtacatct
     6901 gcaattgcgg gccgttatcc gcgcccgggg gcgctctgta cgacgaactc cacgcacttc
     6961 atgacggaga agggcgatga acgagcgttt tgtccaactt gatcgtggcg atggaacccg
     7021 tgttacggcg cgagtccgcg caggtaaggg cccgaatctg attctcgttc ccggcacgtg
     7081 ggggaacatg gacacgcgag ggccattgat ggagcgactc gatcccgaac tcaacattgt
     7141 caacgtcgcg ctgccagggc aggacgacaa ctggccgccg ccgaaagacc cgtccatccc
     7201 cgccttcacc gcggacgtac tccgcctcgc ggatcatctc ggactggagc gcttctttgt
     7261 cggtggaaac agcctcggcg ggatgatttc catcgacatg atccgcctcg ccgagcaacg
     7321 gctgctcggt gtcatcacgc ttgagggatg gacgcactgg agtgctcttt acgacgcatt
     7381 cggggataac acggaggtca cgctgaaccc gcagcagtca gccttcctgc aggaggtcag
     7441 gcggaagctg ctcgaccggt gggaccccgg catgcgctcg cggtatgcga cgatgtggcg
     7501 cgagtgggac gggagcgaaa cattgcaaaa ctgcaccgtt cccctcctct ccatctgggg
     7561 agaccgcggg aagccgcgcc cggcacctga caaactcaag gtcccggtca aacccaatat
     7621 cgagttcctc tgggtggcca acgcgtcgca cgatttgctc gtggaggcac cggaaatcat
     7681 tgccgacgcc atgaacgaat tcatccggcg gcacaacgca tgaaccgtgg gtgggtgccg
     7741 gcgttccgtc tgcccggtgc gggctgcccg ggccggcgcg gttcgtcacg cctgcaaaca
     7801 cctcgcgcaa accccctgcc ttgatctcgc acgcggggaa tagcaacata cgtgtgcccg
     7861 tgtggagttt ccgtaatgac ttcatctgtg gagggtgttt ccatgccgtt gtttccgacc
     7921 gacctccctg agcgcgaatg gtgccagttc aacgcagagg gtttctctcg ccccgtgtgc
     7981 ggcctgatcc actgggggac caacgctccc gtgtgcggaa tgccgctcgg aggaatagat
     8041 accggttgtc ttgacctcga ggccaccggc gtatggggct attgctcaat tttcaactcg
     8101 ctgaatcctc gccgtggcgc gatcaatgaa ccattcctgg gcatcagcat cggtcggcaa
     8161 acgtgggttc tcagcacggt ggcgctctcc cggcgcgacg ggcaggaatg gatcgatacg
     8221 atgcgttcct gggccaatta ccgggccgta aagagtgcct cggacattca ctactgggga
     8281 cattttccgg tcgctgacct ggaatacgag acagacgccc cgctctctat cggcatgcgc
     8341 gcgtgggccc cgttcatacc cggcgacgta gccttgacga acgcgccggc cgcggtgttc
     8401 gaggtgcatc tgcgcaactc tacggaaacc gagcagaagg gcaccctggc gttctcgttc
     8461 cccggtccga acgaaggcga agcgggcacc accacctttc ggcgggtcaa gatcgaaggc
     8521 gccgccaccg gtgtgtcagt tgagtcgcca caggcatcgt acgtcctgac cgcaattggt
     8581 gaatcctccg tgcggacagg tggctgcctt ggcactgatg ctgtcgcgtg gagttgcata
     8641 gaagatgccc ttccgttcgt cgaagccggc gcaggaacga gtatcgccgt ggatttcgcg
     8701 ctttcgcccg gcgcggagaa aacgatacgc ttcgttctcg cgtggtttgc gcccgtctgg
     8761 aacggcggtg gaataatgac cggaaagggg caggattacc gtcacatgta cgcccgccgg
     8821 tacgcaaatg cccgtgccgt tgccgaaatg atcaccggtc gccacgagga aattctgcgg
     8881 aggatcatcg cgtggcaaag tgtactctac ggcgatgaag ggtaccctgt ctggttgcgc
     8941 gatgcgctgg tcaacgttct tcacctgata ccggaaacat ccatctgggc gcaggcaaag
     9001 cccccggtcg gcgactggtg caacgaggaa gacggggtct tcgggatgaa cgaaagcccg
     9061 cgggcctgcc cgcagatcga gtgcataccg tgcagttttt acggcaactt tccgctcgtg
     9121 tatttctttc ctgaagccgc actttccacg ttgcgcgcgt acaaggcgta ccaatacccc
     9181 agcggccagg ccccgtgggt atttggagga tgcaccgtcg ggtctccccc gtacgaaatg
     9241 gccatgccgt cccccggata cgcaaaaaaa ccgcagacga cgctcgacgg cggatgctac
     9301 gtggatatgg ttgaccggat gtggcgccgg accggagacg accagcttct ccgcgagttc
     9361 tacgactctg tcaaacgcaa cacgatcttc accatgaatc tgcggcccgg ctccggcgcc
     9421 gcggggatcg tcagcatgcc ggaggggaac caggcacagg actggtttga aagctgcgat
     9481 ctgttcggga tcgttcctca catcggtggg gtgcatcttg ctcagattcg catggcgcgc
     9541 cggatggcca aggccgtcgg cgacgaggaa ttcgtgcgcc agtgcgattc gtggctcgct
     9601 cagggaagcg aggttcttga aaacgagaca tgggcaggaa cgcactatct cctgttcaag
     9661 gaaaaggaga ccgggaagga atccgacatc attatgagtt acgtgttcga cggcgagtgg
     9721 atggcaaaac tccacggatt ggaaggagtc tttcgccccg atcgagtgaa gacgatgttg
     9781 gaaactatcc gacggaccgc aatcgccatg tcgccgttcg gctcggtggt gttctgcaaa
     9841 ccggaaggtg cgctgggcaa ggaagactgg gatcccggct actggggcgc ccacggtgtt
     9901 cacccgcccg gcacgttcat gctggcaatg acgtatctct tctatggcga aaaagatacc
     9961 ggccttgatc tcgctcgccg gaccatgggc gaaatagtga aacgcggatg gtactgggat
    10021 gttccggtcg caattgacgg cacggctccc cggatcggat tcgattacta ccagaacctc
    10081 gtgctctggt cgttccccgc ggtcatggaa ggcgcagaca tcgcggcacc gtgcaaagag
    10141 ggcgggctgg ttgaccgcgt catcaaagcc ggtgcggagg cgtgatcgcg tcaaaatcgt
    10201 cttcagggcc tgtgaattga cgctgagcac ttttttgaac ctgttgtaga cacctttgcc
    10261 tttgcgctga aggtctgcgc acgtgcatga cgcgaaaagg ataccacgat ggcaactgca
    10321 ggcacgccgg ttcacgccgc tgcgactctt cccgtgcatc ttcccgcgaa gctttccatt
    10381 tgctgctgga tatggagctg gatcacctcg gcaacggaag gcgagccgta ccatgacctc
    10441 gacagatgca tggcgggtct caaagagcgg ggattcaacg cggtgcgtgt ggaggccggc
    10501 ctgaactggg cattcagggc ggatggttca cctcgcgggc aagttgcgtt ccgtccgtgg
    10561 attgcaggtt atggctggaa tttcagcacc gtgaactcgc gcgggggcgg gcggcacgat
    10621 gttctgcaac gcctcgtggc gctgtttgaa agcgcgcgga ggcatggcgt atacgtgatt
    10681 cttacgagct gggagtatca ggacagcact tcgtttctgg ccgaccctgc ccttcgggcg
    10741 gaaatccatg cggtgccgcc gggaaaccgg tatatgcacc tcgcgaagct gcatgaccgc
    10801 ctccttgccg tgctggaaga acgcgggctg gagcagaatg tcgcatttgt ggaagttcac
    10861 aacgaaccgg acgccagcga gtttcccggt ggagaggaag gaagggaact tcacgaagaa
    10921 gcgataggat ttctgcggga ccgccacccc gaagtcctca ttaccggcga tttcacctca
    10981 cacgactacg ccatcgttcc ggagaacgtg caggtgttcg atcagcacgt gtatgccggg
    11041 ttcgggtggt atttccggac cctttttggc gagactgtgc tgaacaagga tttcgacccc
    11101 ggcaaaccgc gcgctctccc ggctctcaac cgcgtactcc gaagggacct tgtcccgtgg
    11161 agcgagttca tggtggctgc cgaaaacgtg cgcgagttct ggcgccccat catgtggatg
    11221 tacgaaaacc tggataacga gaaatgggat gaatgggtcg cggaaacgtt taccgaatgg
    11281 gcgccccgga tacgcgaaaa agcggagacg gcgttcgcct cggatgcacg ggaaggcaga
    11341 cgccggggcc tgccgctggt gttcgacgag ggagggtatt tctatcctcc ccgactggcg
    11401 cgtttcgagg tgttcgggga aggtctttcg ctttttgagc tcatgtgcga tctggcgatc
    11461 acccacgggt actgggggtt catgccgggc acgtattgcg ggccggagca cctcgcctgg
    11521 caggagaatc ccgcgtggct tctcggcatc aacaccagat tccagaaagg gattctttcc
    11581 ggttcctgaa agaaaacgcg tcgcctgaag agactcaggc gacgcgcatc ttcgaaactc
    11641 agtggtcatc tacgccgcgt gcgataccct cgccccggtc gctggcccag cggtcgggtg
    11701 atagcgacgc cgctgggaac tcaacggtcg gtttctcccc gaccttccgc tgccgaatct
    11761 cgaactgccg ccggtaccct gttgagccgc acgcttgcgg tcccgcatgg ccgaaattgc
    11821 cgtggaatgg aatgcatgcc cgtccacatc agaaagcggg caggtggtaa gcttttctat
    11881 tgcgaagagg agtgaaacct cgtcctcccc gcagaacgaa agtgcggttc caacggcacc
    11941 ggcgcgggcg gttcgcccta tgcggtgcac gtaggcttcc ggctcgttcg gcagatcaaa
    12001 gtttatcacg tgcgtgattc cgtccacgtc aattccgcgg gcgacaatgt ccgtagcgac
    12061 aagcacgttc gtccttccgc ggtcgaacgc ggcaagcgcg gtctgccggg cattctgact
    12121 cttgttcgaa tgaattgcct cggcgcggat gcccttgcgc tccaactggc ggacgatacg
    12181 atccgcgccg tgctttgtac gggtgaacac gagcgctctg cgcacagaat cctcgcgcag
    12241 aatctccgtc agcagggaaa gcttatccgt ctgctccacg aagagaacct tctgcgcgat
    12301 gtggtccgaa acggatgcca ccggcgtcac ggccaccatc gccgggtttt tcaccatgtc
    12361 cgacgcaagt tcggcaattt ccggcgacag tgtggcggag aaaaacagcg tctgccgggt
    12421 tgcgggggtc gcgctcacga tcttccgaac gtcgcggaca aatcccatgt cgagcatcct
    12481 gtccgcttcg tccagaacca gaatttccag ccgatcaagc tgaatcaggc gttgcccgaa
    12541 aagatcgagc aatctgcctg gagttgcgac gactatgtcg gggatctccc gaagtgcttt
    12601 cgcctgtggt gcctgtgcca ctccgccgag caacacggtg aaccgcaaag ggagatatct
    12661 gccgtaggcg cgcaggcttt cgccgatctg gagcgcgagt tcccgtgtag gggtcagaat
    12721 gagggcgcgc acatgcttgt tgctccgacc ttcgcgtgtg ttcagaaggt gaagcatcgg
    12781 caggcagaat gccgcggttt tgcctgttcc tgtctgggcg gtcgcaagca gatcgcggcc
    12841 ctcaagaaca cgcgggatgc tgtccagttg aacgggtgtg ggggtctcgt agctctggtc
    12901 caccagtgcg cggaggattc gatcgtcgag accgagtgaa tggaatggat tggtgttcat
    12961 gaaaaactcc tgtctttctg taccgagacc agcggcgact aactctcggt gatacagtca
    13021 gaacaggccg gcgatcgtca agaggggttg acgggggcgg gcatgtgagc cgagaaataa
    13081 cgcagacaat cttcggaagg tttgctgggc accggtattg tcccgtatgg actgattccc
    13141 gatacccgct tttcaaatat acacaaaatc gcgatttatg caaccatggg ctgggacggt
    13201 accctttcgg agccgccacc gtacttcccg gaagctccgc tcaggcgtgt ttctggagcg
    13261 ccggtggatc atgatccctc gcaggaaacg ccggcaaggg cgacggaggc ttttgccttt
    13321 gaggcgctca cgctgcgtgc gatacttctt caagcccgaa acgcgtcttc ctgactcgcg
    13381 acattggagt gcaccatgca tcgcatgaca attccatgga taacgacagc acaaacaact
    13441 cgcggcggtc gtggagggac aaccacggcg gaacgcacca tcgcgctgaa atgtgacctt
    13501 ccggatgaac ccgcggtgcg cgttacgggt gaattgacgc ttgcgcgccc ggaacgcgag
    13561 gccacccttt cggtcgggtc atccgtgtgc acgctgggag tgagcggcga cgcatccgac
    13621 ccctacctcc tgcacgcacg ggcatgggtt tcggatccag attccccccg cggtaccgtg
    13681 gcataccgcc ttgttcccga tgtcacgcgt tgggctcatg acgaagcggg ccgcgagcag
    13741 taccgacggg aatttgcaca tgcgaaggga tgggcggaac acagaattcg gctctccatt
    13801 gacgtcacgc cctgcgaggt tcgcgcatgg gttgacggcc ggctcgtggc ttcgtggagc
    13861 gctcggaacc cgcaacccgg tgttgccttg gtgcaccttc ccgaaggcgc gcgtccgcac
    13921 ggtgcggctg aggtaaggat cctcccgggt gaagaggagt tcttgcccct tgatctgcac
    13981 ccgtttgcgc gaggcgaaga cttcttgaac ctgaaggaaa cggcgtatcg cggtcgcgca
    14041 cctcgtgtgt ttgcctcttc tttgtcatct gatccaaaac gcgccctctt ccgtataccg
    14101 aggaagacgt acgatcgcgt gcacctcgtg tgttccgcca gtactgaggg gggtgctctg
    14161 ccctccgcat cggtggcgtt catcaaaccg gaacgcggcc ataccgtgtt taccgaattc
    14221 ttggtgaatc ctccgtatca cacgccgaag acaatcacgg tacctctcga tccatgcgcg
    14281 ttcagcgatt tcctcaacga ccccggcgag gaagcgctgg aggttgagtt ctgccagagg
    14341 atggattacg acgaaggccg ttttccccat cccgccggcc cgccatccgg cgtccggatt
    14401 cacgagataa cattcgaaac cgcacccgtt gcgctccgtg ttacctcctc cgcggtgggg
    14461 catgtgtttc aggggaccaa aactcctcgt ttctttgttc acctcgagag ccagcggagt
    14521 gttcccggga cggtgcgcgt cgcggttgag gttgcggaac cgtatggtgg gaagtcaaaa
    14581 agctcagcca gagtcaggct ggcgccgtgg gaaaaacgaa cacagacatt tgaactgcct
    14641 caacgcatgt acggccgttt cgggctcacg gtcaccgtct ctcaggaaga tgggtcgcgg
    14701 ccgctgaagc gttcgaccac attcgccctt ctgccgagag acacgcggaa ggccacctca
    14761 gactcacctt ttggcatatg gtgcttcttc gaaaaccacc acggggtgcc accagaaatc
    14821 gccgctccgc ttttccgaaa agcgggtgcc cgttggacgc tggccaactt cgtggtgcgc
    14881 gacgatccgg acgaaacccg aaaacgcgtg cagacgctcg cggctcacgg tgttggcctt
    14941 gcctgtgcga acgttgcgtg tatcggcaac acatgtaacg ccggcggaaa cgacgtagac
    15001 gccatgattc gccgcatgca tgcgatgccc ccggtcaagc actggttgct gttctgggag
    15061 accttcctca gccgccagca catgcgcgac tatccttttg acattctcgg caggccggtc
    15121 cgtgagctca ctcccgatga ggagagcgtg ttcaagaact gctgggatac ggcgattgag
    15181 tacgccacac gcgtgagaaa ggaattcccg gaggcaaagc tcatcttcgg gaacgggcat
    15241 ccgaacttca ttgcagcgtt cctgcggcgt ggatttccga aggaatacat cgacgggttc
    15301 gggctcgatt tcgacatgtt cgtctcttct ccggaattgc agcccggtcc tctccattgc
    15361 ccgttcagcg ggttgttctt tctgaaaaca cttcaggacc tgtacggtta ttcggaaatc
    15421 ccgcgttacc tcaccgaggc aatctactgc tcccagtccg aaggatggct cacagagcgg
    15481 caacaggcgg atcactacgt ccgttctcac cttctcggcc tcgcaagcgg ggttgtgctg
    15541 ttcggtatga ctgccgaact gaacgatccc gggagtgagt acttctacgg atcatacggg
    15601 ccggtagggt tcctgcgccg tgctccggag ctgaatcccc gtgagagctt ctgcgcgtat
    15661 gcaaccatga cgaggatgct ggatcgcgcc aacttcactg agatggttcc catgccgtcg
    15721 cccggcacgt acgggttgcg cttcacaaaa gctgacggaa gcgacatcta cgcgttctgg
    15781 acgaccaccg gaacgcgcct gctcactttc ggagcggaaa gcggatcggt gcgcactacg
    15841 gacctgttcg gcaaccagac cgtgattcga aacgctcgtg gggtgttctc cctcaatgtc
    15901 tccccttcgc cagtgtacgt cgaaggggaa ggaatccacg ggtttgttct cggaactccc
    15961 cgccatcgtc ccggcccgaa aacaagcgag ccgatcgtgc gtatgggtga cctgccgtca
    16021 tggcggcacg aagccgaaga cgacgcgtgg atggagcggc tcgggcgcgc ggggaactgg
    16081 gcaatcacgg cgccgacaat ccggggcagg tttgagttct cgctgagaaa ggaccgggcg
    16141 cgcgggcagg tcctccgcgt atcactttcc gaaacgccgg gcgctcatcc tctgcacctt
    16201 cgctacgccg tactccggtt ttcccggaag gtgcccgtga acggagcatt ctccgcgctg
    16261 ggcctctgga tacgcggaaa cagctcctcc ggacgtgtcc tttacgagct ggaggatgcc
    16321 gacggaatcg tatggcgttc cgtgtacagc gacacctaca tagattttga cggttggcgg
    16381 tacgtccaga cgccgctccc gcttcccgct acggaagacg gatcacggct gcagccgtgt
    16441 gggctcacac cgtgggcaac cgagaccgaa caccgccacc cggcattgcc tgtccgattg
    16501 accgcgctga tcatcgaaag caggactcac gtcgtgcggg caaccagcct tgacccggtc
    16561 cccgacacga cattcgacgt cagcgacgtt gttgtcacca aagaaatggt agacccggaa
    16621 atacgcttcc ggtggtaagg aggcggcgat tcccgctcct cctgcgtttg gagcaaaacg
    16681 ccgattcgtg agggagatgc tctcccgtgt gcacgggcag ttctcatccg gcagaggaca
    16741 acgctctgcc ggtgattccc atcatccttg gcccgacggg ttccggcaaa acggcgctgg
    16801 tcgtggacct cgttcgcgac ggacgcctca ccggtatcga ggttgtctcc gcggattccc
    16861 ggcaggtgta ccgttttctg gatatcggca ctgccaaacc gaccgaggaa gaaagacaca
    16921 cggtccctca taacctcgta gacatccttg acccctccga aacctactcc gccggtcgat
    16981 ttcgtcggga tgcggaggca atcatcgcgg agatcctgaa gcgagggagc accccggtgg
    17041 ttgtcggggg aacggggctc tatctgaagg ccctcgtgga tgggctcagt cccattccaa
    17101 gggttccggt tgacgtggtg gacagactcc gagccgagat cgcgtcagtc ggcccgcccg
    17161 ccctccacga acgactgcag gaattggaca gccgttgtgc agcgacgatc cagccgaccg
    17221 atactcagcg catcatccgt gcgctcgccg tccatgaggc aacagggaag ccactctcgc
    17281 gctggtggga ggagcatccg gagaagccac cttaccgtta tgtctggctg ggaatccggt
    17341 gggatcgctc gcaactgagg gaccgcatcg cccggcgaac tagggcgatg attgcccgtg
    17401 gccttgaacg ggaagtccga tggctgctcg acagggggta cacctgggag atgaacgcga
    17461 tggccacggt cggttaccgg gagtggaagc actgttttgc cggagaaatc acccgtgagg
    17521 aggtgaccgc cgccattgcc attcatacgg cccagtacgc gaaacgccag atgacctggt
    17581 tcagaacaaa cccgcgcatc acatgggttg atggttcgtc gccttccctg aaggatgagg
    17641 tcgtgcgctg gttggatgac cggcacgcgg aggcaaaaag atagccggcg ggcctgtcag
    17701 gcccaccggc cgtgcaatgc gctgcttgtt tcttattcgg ttttgaccgt gatcattttc
    17761 ggtttcgcgt gctctgcctt tggcagagta atctccagca ctccgttgcg atactccgcg
    17821 ctgactttgt ccgggtctac caccgtgggc aggttgaaga cccttttgta cgtgccgtac
    17881 aatcgctcag cacggtgcca ggagaacttg ttggcgtcct ccatcggctt gcgaacaccc
    17941 gtgatggtga gcgtattgcg ctccacgctc aactggatgt cctcacgatt gagcccgggg
    18001 aggtccgcgt agatccgaag attgtcagca tcctcaatta cgtctacccg gggcgcccaa
    18061 tcgctcaggg tctcgcctga aaactcaccg aagcgcgaag acagcgcatc gaacaggcga
    18121 ttgatttcgt tctgaatgct gggcagagtc ggtaccatcg ccatatccaa tccctccttc
    18181 ctcacacaga agtaatcgac tgaatgtaga aggcctgagc catcttcacg gcctgtacat
    18241 cgcccattgc aaccgctgtg ccagtgattc gacggcgccg tggaaagacc gcgcagaagc
    18301 ctgttggcgc ggtacccagg cacaaggctt acatcccgca cgctgtcata atggcgcctt
    18361 acccgcggtt gtggcaaaga tgatgccgtt ttggcagggt gcggcgcaca aggtggcacg
    18421 gtgaaacgtt tttgcgatat ttcatcctgc gcgtacccat tcccacgttg gcgcatcggc
    18481 cgtaacagat cagccgtaca gaggcatgtg aaatggcatt cgagcgacga acacagcttt
    18541 ccctctggta tttcgtcggt gccttcatga ttctgctggc tctgaacctg tatttctcaa
    18601 aatctcaggt cgaaccgctg aattacagcg tgttccggca gtatctgaaa cagggacgta
    18661 tcgttgaggc gactcttacc gcccagcata tcaccggtgc gatgcgaacc ggtacatcaa
    18721 cctccacgct tgcccgtttt gtgacggtgc gcgtgacgga ccctgagctt ctggaccttc
    18781 tcacccagca tggagtcatc ttccgcggac gcgtggaaga cacatgggta accgaaatgt
    18841 tggcgtggat cggtggcatg gcggtgtttt tcgtcatctg gatgttcctc attcggcgca
    18901 tggggggacc ctccagcggg atgatgtcca tcggaaagag caaagccaag atctacgtcg
    18961 agacggaaac caaagtgacc ttcgccgatg tcgcgggaat tgacgaagcg gaagaagaag
    19021 tccgcgaagt ggtggaattc ctgaaagaac cggagcgttt ccggaaactc ggcgggcata
    19081 ttcccaaggg agtcctgctg gtcggtcctc cggggaccgg taaaaccctt ctggcgaggg
    19141 cagtggccgg ggaatccggg gtgccgttct tcagtctgtc cggatccgat ttcgtggaga
    19201 tgtttgtcgg cgttggcgcc gcgcgcgtgc gggatctttt ccagcaggcg aaggagcggg
    19261 cgccctgcat cgttttcatc gacgagcttg atgcgctcgg caaagcgcgg gggatgaatc
    19321 cgctgggaag ccacgacgag cgggaacaga ctctcaacca gctgcttgtc gaaatggatg
    19381 gattcgatgc gaacgtaagc atcatcatca tggcggcgac gaaccggccg gaaatccttg
    19441 acatggcttt gctgcgtcca gggcgtttcg atcgtcagat cgtggtggat cggcctgatc
    19501 tcaacggtcg cctggaaatc ctgaaaatcc atgcgaagcg cgtgaaactc gacccggaag
    19561 ttgacttacg gcaactcgcc gcgggaacgc ccggaatggt cggggcggac ctcgccaacg
    19621 tggtcaatga agcagcgctt ctggccgctc gacgggggca cgacgcgatt tcccagaacg
    19681 atctccagga ggcggtggaa cgcacgctcg tcgggcttga aaagaagagc cgcgtgctga
    19741 atgaacgcga gcggacgatt gtcgcatatc acgaggccgg acacgcgctt gtgggtcttt
    19801 gcagtccgca cgccacgccc gtacacaggg tgacgataat tccccgtggg gtggccgctc
    19861 ttggctatac gatgcacctc cccacggagg accggtacat ccagacgaag gcggaactgg
    19921 acgccgaact gggagtcgta ctgggcgggc gcgcggccga agaagtggtg ttcaacgaaa
    19981 tcagcacggg cgcgtccaac gatttcgtgc aggcaacccg aatcgcttcg agcatggtca
    20041 aagactacgg aatgagccgc ctgggagtgg tttcgtacga cgacgagaac caatcccggt
    20101 tcctccgcca gatctcggga ggcatgcctc agtacggaga acgcacggcg gcgcagattg
    20161 accgcgaggt gcgcgcgatc attgatgagc ggtatcagca cgtcaagaag cttctaagcg
    20221 aaaaaaggga acaactcgaa gccgtcgcgc aacgtcttct ggcgacggaa agcatatctt
    20281 cagaagagtt gcgcgagctt gtcgaaggat gtggagcgaa caaaccggcg caggaggtgc
    20341 cagactctgt tcaaggggag ggagtcacct ctgacgcgac aaaggaggac gcatgacagt
    20401 tgacgatctt tccagcaagg ccgcgtcact gcggcgtcgc gcggtcacag tttcgcgcga
    20461 acactggggt tatgtgttgg tggga
//
LOCUS       DAOSOO010000033        20403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2617_length_20403_cov_5.331925, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000033 DAOSOO010000000
VERSION     DAOSOO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2617_length_20403_cov_5.331925"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLT86_04060"
     CDS             <1..829
                     /locus_tag="PLT86_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35824.1"
                     /translation="IAGERVRSGGNLQIEVGRVSAVARESGWLGKFHRFEITYRVVPD
                     VPYISVHVRIWPEFAEGTPGAPGRAGDPGEKVRFVATLNPDLGPIRCVRQQPLLIWEY
                     DPSLSPVFAALEWVDYQGTDLGLALLNDGAIGQSVDGTASEVGAILLNGGISDYHGDL
                     AFMPHRSDWRGGTVHEAGQCFGSPLNGVFEVSHPGRLPKRFSLASVTPRNVTVSSVFR
                     ERGKTYARVWEHSGERARVQFSREGVLLQAERVNLRLRKCAGGSNLEPRRIAVFRIG"
     gene            complement(892..2328)
                     /gene="dnaA"
                     /locus_tag="PLT86_04065"
     CDS             complement(892..2328)
                     /gene="dnaA"
                     /locus_tag="PLT86_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT35825.1"
                     /translation="MTCSPAEAWTQCLVALKGKLFSQSYDTWIKPLVCTRFDSELVVL
                     EAPYSFSVSWIREHYLRDIERTIRKLFNISPRVEFELLPGSSDEETRLLSGDPHVQIG
                     PGTSDRHAATSRKSAPRTDKEPAETETLSLVNKDYTFGSFVIGSNNEVAQSAAKTVAR
                     NPGRTPFNPLLIYGGVGLGKTHLLHAVANECALAGNAKNVIYLTSEQFVREFVTSLKE
                     NRTLQFARRYRQADVMLVDDIQFFQGKERMQEEFFHTFNTLHGQGKQIVLSSDRPPEA
                     LGGLQDRLLSRFQWGLVADIQPPDLETKIAICEMKAEATGLHLDDSAAEFIASQVKSN
                     IRELEGIIKRLLFLVQNHDAKLTLDIVESAVRTMRLAAPETVTVKRIQEAVASHYGIS
                     VDLLTGKSRRSDVVSKRQLAMYLCKLLTTESLRAIGAAFGGRDHATVIHACERIETAL
                     AEQRSLVLELNSISVALGVSGRIRTNVA"
     gene            2802..4535
                     /locus_tag="PLT86_04070"
     CDS             2802..4535
                     /locus_tag="PLT86_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35826.1"
                     /translation="MRNHLSADDFKTRIQWHPEDAVIRVDGRRKILVDVDAMHALRET
                     IVNTVGQERARGIYRRFGYGCGKADFEQAAATNPEASPDDLLALGLQAHMFMGHVHAE
                     LTETRLDMEKKDLRAEGVWRHSFETDCGVPLGPSSCWMLQGYASGWASALVGKPVVAL
                     ERQCTGAGAPFCIFELRTPEKPFGPEQVFLDDLGQVNLEDHIGLLEQLAADLRTAQTR
                     LMGSEAKYRALFENAPDMMMLCDPVTGRILDVNRAVVERLGYERDAVLRMTLRDLHPQ
                     KDHATLSKVIGPSITDPVGSSPMQLVGSGKQVCSVEAAFSLVPFAGSTVLQAIYHDVT
                     GFQTAQLALREAEELARIGRMASAVAHEIRNPLSAIVSGIRLLTSSKRSEEERSLIFD
                     TILVESERLDSTLNDFLQFARPRNPRRKPENLEEMLQDLVRIIWSDKDAVGSVVPRIT
                     VSPDLPLVNCDGDQIRQVFWNVILNAIQAMAGQGELAIDVTYENGLVRVSIADTGPGI
                     PPEELERVFEPFHTTKPRGTGLGLPIARRIIQAHGGAIAVESQPGAGTRVTFTLTSEG
                     IDGNSQSSARR"
     gene            4504..5883
                     /locus_tag="PLT86_04075"
     CDS             4504..5883
                     /locus_tag="PLT86_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT35827.1"
                     /translation="MATPRVLLVDDERAVVGLLALALKDAGYHADVEHTGTAAVGRLS
                     DEVYDAVITDLRLDDMTGIDILRIACKRYPSPAVLIMTGYGSVDSAVEAMKLGAADYL
                     TKPVYPQELLLTLERALQHRQLLSELDALRQRVGQLQLDDLIGISPAMQTVFRTIQRI
                     ARTDVTVLITGESGTGKELVARAIHNHSSRAAHPFLTVNCSALTETLLESELFGHAKG
                     AFTGAIVAKKGLFEDADGGTLLLDEIGDMELGAQAALLRVLESGEVRRVGETRPVGVD
                     VRIVASTNRDLENAIREGKFREDLYYRLRVVPVDIPPLRKRLDDVLPLAQHFLARYAP
                     KFNRGHVRLAKETGEALLRYTWPGNVRELEHAIERALLLSDGDVLLPEDLPPEVSSPP
                     ASRANTTQLTLAEMERQHILERLDQCSGNRSEAARQLGISRNTLARKLKSYGFSDEET
                     NEASDEAFP"
     gene            6094..6474
                     /locus_tag="PLT86_04080"
     CDS             6094..6474
                     /locus_tag="PLT86_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35828.1"
                     /translation="MLSVLVVDDETGARTMLAMALRGADIHVDMAADGPTALELIGKN
                     PYDWVVSDVKLPGMDGVTLIKQIGRLSPKTRMLLISAVLHEDDVRALPIDGFWRKPFD
                     PFAIQRFIVENANAADSRKAYGLR"
     gene            complement(6459..7322)
                     /gene="pyrF"
                     /locus_tag="PLT86_04085"
     CDS             complement(6459..7322)
                     /gene="pyrF"
                     /locus_tag="PLT86_04085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT35829.1"
                     /translation="MPYPHNVPIPRRSNVDFLAKLKAAARTRGSWLCVGLDPIVEKLP
                     ACLPRSSTGVTAFCRRIIESTAPHACAFKPNIAFFEALGRDAFSILEDVISSVPEDIP
                     VILDAKRGDIGSTAHLYARSYFEKLGVDAVTLSPYLGWDAIEPFLGYPGKAGFVLCLT
                     SNPSAAQIQMLPCEGEPLYVKVARLVQSQNPSTGLVVGGTRPEQIGIVREICPESPFL
                     IPGIGAQGGDLELSVRLGSWKTGGGALINASRAVLYASNGEDFAEAAGGVAAGLKEEI
                     ARAGSAFQRSP"
     gene            complement(7282..9492)
                     /locus_tag="PLT86_04090"
     CDS             complement(7282..9492)
                     /locus_tag="PLT86_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOT35830.1"
                     /translation="MPETPISSNDTLRGVLDHVTFRNDDTGFTIARLVREPDKVPVTI
                     VGTIPACTIGETVEVSGEWKSHPSYGEQFRLTSYRTILPSSSEGLKRYLGSGLIKGIG
                     PAFAGKIVRKFGAETLDVITKHPDRLREIEGIGPTRVRQIQAAWREQADIRELMLFLQ
                     HHEIPTGVAHRIYRKYGAESVAVIKADPYRLADEISGIGFKTADKIAEHVGIGQDDPR
                     RIEAGIKYVLNRSTEQGHTYLPADELTQETAQQLSVPVSAILPIMERAARAGEILFEA
                     NRVSLPRYVFCERQIAQCLADLITHHPSPRNNEIQASVIARVESRTGVQYAPEQAAAI
                     MASLQEGVLVITGGPGTGKTTTTQGIVSELVAERKRLLLCAPTGRAAKRLAEVTGHEA
                     KTIHRLLGFVPGGHEFQHDERHPLPADVVLVDEASMVDTELFLHLVRALPSGCRLILV
                     GDADQLPSVGPGQVLRDIIASQVVATVALRTVFRQAQASMIVTNAHAINQGVFPQLPN
                     TREGDFFFVYREDPEEIAATIADLVTRRLPFRYKLNRFTEIQVLTPMYRGETGANALN
                     KLLQEKLNPGGASVPRGTTEFRVGDKVIVTRNNYSKMVFNGDIGRIVGVDAENAKVSV
                     ELNLSAADTVRVTYEFDELDELSLAYAISVHRSQGSEFPAVVMPITTQHYMMLQRNVL
                     YTAVTRAKRLLVLVGSKKALAIAIRNGSATGRYTMLAQEIRDAVSAQRAHSPEK"
     gene            9618..10007
                     /locus_tag="PLT86_04095"
     CDS             9618..10007
                     /locus_tag="PLT86_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOT35831.1"
                     /translation="MAHEVVVAKTEEWQDGSLGIIEVDGHRVALCRVGDAFFAFDDRC
                     PHAGSTLGLGTLHANAVECPRHQYLWRVTDGAPLTTAGPLTLFQVRVEGDSVLLTVPD
                     RWPEPAHWARHPKREELRRRHGFEEGE"
     gene            10042..10779
                     /locus_tag="PLT86_04100"
     CDS             10042..10779
                     /locus_tag="PLT86_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT35832.1"
                     /translation="MKALVLAGGYATRLHPVTLEASKPLLKVNGRPIVDYVLDGIETL
                     GDVDTTYVVTNAKFFQDYVEWAKTQQRPFDVVPLSDGTTSNADRLGAIGDIQFAIETQ
                     SVDDDLLIVGGDNLFDFSLRPLRDLQRAAGRSVLGCYDVGSLDLVSLYSEVHIEKGLV
                     SSFVEKPETPTSPLIGILCYALRRQDVPLVRRYLEEGRGPDKAGHLIQWMRTVTEVAA
                     YVFEGRWIDIGTRAELDKAEKQWSCPA"
     gene            complement(10782..11225)
                     /locus_tag="PLT86_04105"
     CDS             complement(10782..11225)
                     /locus_tag="PLT86_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35833.1"
                     /translation="MTEAQAYASELGNPKEGETMVEITQGTDPGICIVRFSQGARICG
                     QLADLLNPVDPATFHQYRLPARTPVELHFHDFDEYWWFVEGHPTVTMRGKDGRMVEEI
                     LQPPDMVACVRGFEHTLYADHDLVYYQFSSVRTGSEREGHLSRAG"
     gene            11320..12582
                     /locus_tag="PLT86_04110"
     CDS             11320..12582
                     /locus_tag="PLT86_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT35834.1"
                     /translation="MSDRLAIDGGTPVRTAPLPPSWLGAYEMGVEEEEAVLKVLREKR
                     VFRYIAKEESETARLEKAVAEFTGRDYALAVNSGTAALICALVGLDIGPGDEVIVPGY
                     TYIATAAAVVTVGAVPVIAEVDNSLTLDPEDLERKITGLTKAVIPVHMRGTPCRMDEI
                     CAIAKKHGIKVIEDTAQANGGNYKGKTLGGIGDVGCYSYQMSKTITAGEGGMVVTGDR
                     LVFERILMAHDSAFGFWKPDETAIRPIPGEGFRISEVSGAIALVQMGRLRGIVERLHN
                     AKYRIVNQIRGLAGITLQDVPDESGDASVCLMFFAPDAEIANTYAKALNAEGIPCGTM
                     FSKEIPDRHIYPNWTYVLEKRGRSSNWSPWHPSVYKGSVEYSKDMCPKTLDYLGRTIH
                     IGLSPRLTAADSDDIARAIRKVVTALAR"
     gene            12672..14249
                     /locus_tag="PLT86_04115"
     CDS             12672..14249
                     /locus_tag="PLT86_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOT35835.1"
                     /translation="MNYPFWDVPLLGGGLVIALIAIPHVFVSHFAIGGGIFLAWTERI
                     GIRKGKPEYIAFAKRNTLFFVLMTLVFGAVTGVGIWFSIGLVHPDATSALIHNFVFGW
                     AMEWVLFLVEIAAALLYYYGWDRVDQKTHQFIGWAYAGAAWGSLVVINGILAFMLTPG
                     RWVETGGFWTGFFNPSYFPSVLIRTCVCLALAGLYALLMASREPAGETHDKLVRYAST
                     WLLPAFIGLPIFAAWFVFTLPSLAQDMFMGRAVVLTIFAALSLGISAIIFAFAYLIAY
                     RNPHSVSTPVALLFLVLGLAVTGVTEFGREAARKPYIIYDYMYSNAVLKRDVARLQQE
                     GVLRNAKWALVSKATPQNALIAGREIFTLACAHCHTVDGYNGVRLLVKGWPEPFIEHQ
                     LTYLDQLKGFMPPFVGNPEERRALAKWLASLNPSALPARTATAATDSLSADPLARGEA
                     AYSAHCANCHTIDGRNAIRQHVKGWTQSIAYERLGRLESLRGYMPPYEGPEAERQALA
                     AWLASIGNDAPAAGGAR"
     gene            14246..15358
                     /locus_tag="PLT86_04120"
     CDS             14246..15358
                     /locus_tag="PLT86_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35836.1"
                     /translation="MNEAIIPSIDPIALPAPVWLMQFLSGLTFIFHVIPMNVVLGGGV
                     VALFLAVWGTRGSRKNHVRLARRMTSVLPAAIAMTITLGVPPLLFLQAMYGPLFYTSS
                     VLMAWPWLSVIAILLVGYYGHYYFSLSKDFANTRTLVVGAVAVVAFLVIAFLYVSNMT
                     LMVMPERFLGIQRSWTSGFLPFTGDSIFAPRFLHFITGAVSVGGLLAVFVGLLERSDA
                     EFKAVAVRAGGFTFVGATIVQIATGIWFLIALRPRVMMLFMGQDHVATALFGVAVLLA
                     LVAVALILVAVIGKKRPWMTIAGTACIGLTIIIMTVMRSIVREAYLGTRFVVAEQPVN
                     PAWDVVVLFLVLLVAGAALVGWMIHAVVTRRGLAAS"
     gene            complement(15435..16313)
                     /locus_tag="PLT86_04125"
     CDS             complement(15435..16313)
                     /locus_tag="PLT86_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35837.1"
                     /translation="MEPLSIAWTMDCERIAAESPPGGPATWELSERAIRGYCDTLLAA
                     EAFPTLFLTPECAARHAAMFRDYAEEGVELGLHVHPQSFGDHKYGRYLGEYDADMQRK
                     IISEAMTVFADAIGWAPRSFRAGNDSATDDTFRVLSELRFLAGSVSCPGREVPKYAAR
                     WETAYRYAHWAHRSNRLVPGDLPFFEVPMSTHPTERSPDGLAHELRIEWGPLKDWQQP
                     IIEAVLADMETNRVAFKSLCFITHNTFAYDDASIPQSQTLRDTLAFLRTLAPRFTIKA
                     TTLTEARETFSTMCPV"
     gene            complement(16313..17164)
                     /locus_tag="PLT86_04130"
     CDS             complement(16313..17164)
                     /locus_tag="PLT86_04130"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOT35838.1"
                     /translation="MAQRTEEHKRQHYDNGLPEHQAVLDDIERVARLLRTSRSVLFIT
                     GAGISADSGLPTYRGRGGIYDGCTAEDGIPIESALSGDTFRKRPEVTWKYIARIESAS
                     RGRTPNRAHEVIAEMEPLFDRVCVLTQNVDGFHAAAGSTNVIPIHGNLHDLRCTRCSY
                     RTRVPDYSGLELPPSCPSCGAVIRPGVVLFGEQLPASEVERLLHELERGFDIVFSVGT
                     TSVFPYIAQPVMRAKARGTPTVEINPETTALTDVFDFHIAERAAVALDELWRRYRAGR
                     LPDTKGN"
     gene            complement(17164..18381)
                     /locus_tag="PLT86_04135"
     CDS             complement(17164..18381)
                     /locus_tag="PLT86_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35839.1"
                     /translation="MNHEVAILRDLVRRYVEVCSRPEQDIRRALWRTHNSLKPTRPLI
                     YVRAFAWSEMPDSRCVISDPFLRHFEDVFRQKLFWSTLDDDSVFEPWVTLTACHRCTG
                     WGVSGERHFSNEPRGSFKVEYPLKSLEDVSRLRIPVHSIDEECTARRYEQLADAIGDI
                     ITINVDRAPAYRMWSGDISTDLGYLRGIEHFMVDMVDNPEGLHRLVQFMSDGILKTHE
                     EAEAAGDWGLSAHQNQAMPYAEELPDPAANANGVKRSRLWGYMAAQEFTAVSPAMHDE
                     FLLQYQLPILKHFGLVAYGCCEDLTHKIDMLRQIPNLRRIAVSPFADVKACAEQIGRD
                     YVVSYRPSPSDMVGYGLAEDRVRAILTKDLEACRDCHVDITLKDVETVQSDPRRVRRW
                     VALTRKVIDEVWK"
     gene            complement(18454..20043)
                     /locus_tag="PLT86_04140"
     CDS             complement(18454..20043)
                     /locus_tag="PLT86_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOT35840.1"
                     /translation="MKCRVVSMNEWLYPDMAAGVSRPKPIVLHAARGTRTGFQVLFKP
                     TPANGELRVSLASQGADFPSPEIFQLVDVMVEKNTGPIGFIAKEGESAEGYTTRLAPF
                     RVYDAMKPFLPGTKGRMGSEALFFEWRIPEDADAATYTSTIRVTIGNESLTLPVKLTV
                     HAATVTDPGTFSLTNWFNLGAMASRHGITPWTEAHWRMIRRYGELMRRGRQTHFWIPM
                     GYIGIKEGPEGLFSFDFAKAERLIRTYLKLGFTGIEGGHVAGRTEFHAPNFVLSTNHK
                     VNAVSREGYNFLAQYLPAWRDFLCRNGWLDIAVQHVGDEPTDKSMADYRVISGIVRKF
                     MPGTPIIDAVELSELAGAVDIWVPKDDYYEKHRDEFEFFRHHGDTIWCYICCFPGGYY
                     MNRLLDMPLLRTRYIQWGCFKYDLPGFLHWGLNHYRHDQNPFECNCPDHGGGNNLPPG
                     DTHNVYPGDDGPWGSVRLEAQAAGIEDYELLRQLKEVDPESALKILSSCFRGFRDADE
                     NPQRFAAAHLNLLKAVSAATD"
     gene            complement(20085..>20403)
                     /locus_tag="PLT86_04145"
     CDS             complement(20085..>20403)
                     /locus_tag="PLT86_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35841.1"
                     /translation="ATPDAAPAFAAAKRLGWHTLAASPFVRGWHLDRLVAAARKSWRG
                     SESDLRRRLADHMLRYSLFHPNVDRVITAMRRVEWVTANVESVHRGPLTASELQWLDS
                     ISS"
BASE COUNT         4058 a         5790 c         6208 g         4347 t
ORIGIN      
        1 cattgcgggg gaacgtgttc gctccggagg aaaccttcaa atcgaggttg gtcgggtctc
       61 cgcggtggcc cgggaatcag ggtggctggg caaattccat cggttcgaaa tcacataccg
      121 cgttgttccg gatgtcccct acatatccgt tcacgttcgc atctggccag agtttgccga
      181 aggcactcca ggcgcacccg gaagggcagg ggaccccggc gagaaggtcc gtttcgtcgc
      241 aacgctgaat ccggatttgg ggccgatcag atgcgtaagg cagcaaccgc tgctcatctg
      301 ggagtacgac ccctcgctct ctcccgtttt cgccgcgctt gaatgggtgg actatcaagg
      361 caccgatctt gggttggcgt tgctcaacga tggtgcgatc ggccagagcg tggatgggac
      421 ggcttctgaa gtcggggcga ttcttctgaa cggcggaatc tccgactacc acggtgatct
      481 cgcgttcatg ccgcaccgga gtgattggcg aggagggacc gtgcacgagg ctggccaatg
      541 cttcgggagt cctctgaatg gcgttttcga ggtgtctcac ccgggtcggt taccgaaacg
      601 attcagtctt gcatcggtta cgccccgaaa cgtgacagtg agcagtgtgt tccgggaacg
      661 ggggaagacc tacgcccgcg tgtgggagca ttcaggagag cgggcccgcg ttcaattttc
      721 gcgagaaggg gttttgttgc aagccgaacg ggtgaacctg agattgcgga agtgcgcggg
      781 aggcagcaac ctcgagccgc gaagaatcgc cgtcttccgg ataggctgac ggaaaccggc
      841 gatctggtgt gcggtgggaa agaaaaggcg ttcagtcgct aactgtggct ttcacgcgac
      901 atttgttcgt attctgccgc tcacgcccag cgctacggag atggaattca gctccagaac
      961 gagggatcgc tgctctgcca gcgccgtctc aatccgttcg cacgcgtgaa tcaccgtcgc
     1021 atggtcgcgc ccaccaaacg cggcccctat ggccctgagg ctctccgtcg tcagcaattt
     1081 gcacaggtac atcgcaagct ggcgtttgct gacgacgtcc gaacgccggc tttttccggt
     1141 caggagatca acagaaatac cgtagtggga ggcgacagct tcctggatgc gcttcacggt
     1201 gacggtctcc ggcgccgcaa ggcgcatggt gcgaaccgca ctttccacga tgtccagcgt
     1261 caacttcgca tcgtggttct gcacaaggaa aagcaaccgt ttgatgatcc cttcaagctc
     1321 ccggatgttg cttttcacct gtgaggcgat gaattccgcg gcgctgtcat cgagatgcag
     1381 gcccgtcgcc tcggccttca tctcgcagat cgcgattttt gtttcgagat caggaggctg
     1441 tatgtcggcc accaatcccc attggaatcg cgaaagcagg cggtcctgaa ggcctccaag
     1501 ggcttcaggc ggacgatcgg acgacaggac tatttgcttg ccttgcccgt gaagcgtgtt
     1561 gaatgtgtgg aaaaactcct cctgcatgcg ctctttgccc tgaaagaact gaatatcgtc
     1621 caccagcatt acatcggcct gacggtacct gcgtgcgaac tggagggtcc ggttctcttt
     1681 caaggacgtc acaaactcgc gcacaaattg ttcgctcgtg aggtaaatga cgttctttgc
     1741 gttgcccgcg agcgcgcatt cattggcgac tgcgtgcaga aggtgtgttt taccaagtcc
     1801 cactccaccg tatatcagga gggggttgaa aggagttcgg cccgggttgc gcgcgaccgt
     1861 tttcgcagcg ctctgcgcaa cttcgttgtt tgagcctatg acgaaggacc cgaaggtgta
     1921 atccttattc accagcgaga gcgtctcggt ttccgcaggc tctttgtccg tgcgcggcgc
     1981 tgattttctg gacgtcgccg cgtgacggtc cgatgtgccg ggtccgatct gcacgtgcgg
     2041 gtcgccggag agcagtcgcg tctcttcgtc cgacgaaccg gggaggagtt cgaattcgac
     2101 tctcggactg atgttgaaca gctttcgtat cgtgcgctcg atgtccctga ggtaatgctc
     2161 tcgaatccac gatacggaga agctgtacgg cgcttccagc acgacaagtt cggagtcgaa
     2221 ccgggtgcac acgaggggtt tgatccaggt atcgtagctc tgggagaaca acttcccctt
     2281 caaagcaacg agacactgag tccaggcttc tgccggtgag caggtcattt cgcgcgtatc
     2341 tttatcaaaa aagggttaag ttcttaataa acaaattctt gaccaagttg cgtttttcgc
     2401 cgtgttgtga tcaacgaaaa atacgtatcc ctcggcgtca aaatcccttc tgaagggcgc
     2461 tgatgccgtt catggcacgg gctgaatcac acgctgagat actcggttag tgtaaggtta
     2521 agctaagggg tcaatcccga aggaaacaat gcatttttga acagtggttt ttcttttttt
     2581 gggtttcctt ggtgttcaaa aattcacacc cactacctgt ggtggaaaac ggtggaatcg
     2641 cattgatcgc atgccgtacc cgcattgcat gcgccaaaat ggcaagttcg tcatttgaac
     2701 ctgcggaaac cgggtgaccc cgtgtcagcg ttgtcttgtt tgaaaggcga ccgatacatt
     2761 tagagcgtga cccggaacgg tattctgcag ggacgatcac gatgcgtaat catctcagcg
     2821 ctgacgactt caaaactcgc atacaatggc atccagagga tgccgtcatc cgcgtggacg
     2881 gacggcgaaa gatactcgtc gacgtagatg ccatgcatgc tctgcgcgag accatcgtca
     2941 acaccgttgg gcaggaacgg gcgagaggca tctacaggcg ttttgggtac ggatgcggga
     3001 aagccgactt cgagcaggcc gcggcaacca atccggaagc gtctcccgat gatcttctgg
     3061 cgttggggtt gcaggcgcac atgttcatgg gtcacgtgca tgcagaactg acagagacac
     3121 gtctggacat ggagaaaaag gaccttcgcg cggaaggcgt gtggcgacac tccttcgaga
     3181 ccgactgcgg cgtgcctctg gggccttctt cgtgctggat gcttcaaggg tatgcatcag
     3241 gctgggcaag tgcccttgtg ggaaaacctg tggttgctct ggaacgtcag tgtaccggag
     3301 caggcgcgcc gttctgcatc ttcgaactgc gaactccgga aaagcctttc ggaccggaac
     3361 aggtttttct ggacgacctc gggcaggtca acctggagga ccacatcggt cttttggagc
     3421 aactggcggc ggatcttcgc acggcacaga cgagactgat gggttccgag gcgaaatacc
     3481 gcgcgctgtt tgagaacgcg cccgacatga tgatgctgtg tgacccggtg acgggtcgta
     3541 ttctcgacgt gaatcgtgcc gtggtggaac gcctcgggta cgaacgtgac gccgtgctcc
     3601 gcatgacgtt gcgtgatctc catcctcaaa aagaccacgc cacactgagt aaggtaatcg
     3661 ggccttccat tacagacccg gttggctctt cgccgatgca attggttggt tcgggaaaac
     3721 aggtttgttc cgtggaggct gcgttttcgc tggttccatt cgcgggatcc accgttcttc
     3781 aagccattta ccacgacgta actggttttc agacggccca actggctctg cgcgaggcgg
     3841 aagagctggc gcgcattggc cgtatggcct ctgccgttgc ccacgagatt cgcaatccgc
     3901 tgagtgccat cgtgagcgga atccgcctgc tcacgtcctc caaacgttct gaggaagaac
     3961 ggtcgctgat tttcgacacg attctcgtcg aaagtgaacg attggacagc acgttgaatg
     4021 atttcctcca gttcgcacgc ccgcggaacc cacggagaaa accggaaaac ctcgaggaaa
     4081 tgctgcagga cctcgtgaga atcatctgga gcgacaaaga cgccgtcggt tcggttgtgc
     4141 ccagaataac ggtctctcct gatctgcctc ttgtgaattg cgacggtgac cagatcagac
     4201 aggtgttctg gaacgtcatt ctgaacgcaa tccaggcaat ggcaggccag ggagagcttg
     4261 ccatcgatgt gacttacgag aacggtctgg tccgtgtttc gattgccgac acaggccccg
     4321 gaattcctcc ggaggaactc gaacgcgtgt tcgagccgtt tcacactaca aaacctcggg
     4381 gaaccggtct tggccttccc atcgcacggc gaattatcca ggcccacggt ggagcgatag
     4441 ctgtggaaag ccaaccgggc gcgggtacgc gggtgacatt cacgttgacc tcggaaggga
     4501 ttgatggcaa ctcccagagt tctgctcgta gatgatgaac gcgccgttgt tggtcttctg
     4561 gcgctggcgc tgaaagatgc cgggtaccac gccgatgtag aacacacggg aacggcagcg
     4621 gtgggtcgcc tttcagacga ggtgtacgac gcagttatca cagacctgcg tctggacgat
     4681 atgaccggga tcgacattct cagaatcgcg tgcaaacgct acccaagccc ggcggttctg
     4741 ataatgacgg ggtacggtag cgtggattcg gcagtcgaag cgatgaaact cggtgcagcg
     4801 gactacctca ccaaacccgt gtacccgcag gagttgctgc tcacgcttga gcgtgccctc
     4861 caacaccgcc agttgctctc cgaactggat gcgttgcgtc aacgcgtcgg tcaattgcag
     4921 ttggacgacc ttatcggcat aagccccgcg atgcagacgg tgttccgcac aattcaacgc
     4981 attgcgcgca ccgacgtgac agttctcatc accggcgaaa gcggcacggg gaaggagctg
     5041 gtcgctcgcg ctatccacaa ccattcctcg cgcgcagcgc atccgtttct tacggtgaac
     5101 tgcagcgcgt taacagagac cctcctcgaa agtgaactct ttggccacgc aaagggagca
     5161 ttcaccgggg ccatcgttgc aaaaaaaggc ctgtttgagg atgccgacgg tgggaccctt
     5221 ctcctcgacg aaatcgggga catggagctt ggagcgcagg cagcgcttct gcgggtcctt
     5281 gaatcgggag aggtcaggag ggtgggcgaa acaaggccgg taggagtgga tgtgcggatt
     5341 gtggcttcca cgaaccgcga tctcgaaaat gcgattcgtg aagggaaatt ccgggaagac
     5401 ctctattacc gacttcgggt tgtccccgtg gatatcccac ctctcagaaa acgactggac
     5461 gatgtactcc cccttgcgca gcattttctg gctcggtacg cccccaaatt caatcgggga
     5521 cacgtccggc ttgcgaaaga aacaggtgag gctctccttc ggtacacatg gccgggaaat
     5581 gtgcgcgaac tggaacatgc aatcgaacgc gcgctgctgt tgtctgatgg cgacgtgctc
     5641 ctcccggaag accttccgcc cgaggtgtcc agccctcccg cgagcagggc caacacgacg
     5701 caattgacgc ttgcggaaat ggaacggcaa cacatcctcg aacgcctcga ccagtgctcc
     5761 ggcaatcgaa gcgaagccgc gcgacagctt ggcatcagcc ggaatacgct cgcgcggaaa
     5821 ttgaagagtt acggattttc ggacgaagaa acgaacgaag cgagcgacga agcctttcca
     5881 taacctggcg cgaagaaacg aaccaaaggg gcaacgaaat gtagcaacgg gcccggttga
     5941 atcaggatct gccagccccc gggtctccaa gagaaacccc gttgatggcg tctttcccgc
     6001 ttctcttcac cttcgctgcg acacccgacc gcacttctgg cacggaatgt ggagtaatcg
     6061 ttggccgcag ggttcatcga gaagggttgc ggaatgcttt cggttttggt tgtggacgac
     6121 gaaacgggtg cgcgcaccat gctggcaatg gcattgcggg gcgcggatat ccacgtcgac
     6181 atggccgcag acggcccgac tgcgctcgaa ctgatcggga aaaacccgta cgactgggtg
     6241 gtttcggacg taaaactgcc gggcatggac ggcgtgacgc tcatcaaaca gatcggtcgc
     6301 ctgagtccga aaacccgaat gctcctcata agcgccgtac tgcatgagga tgacgtacgg
     6361 gcgcttccca tagacggctt ctggcgcaaa cctttcgacc cgtttgccat ccagagattc
     6421 atcgtggaaa acgcgaatgc tgccgattcc aggaaggcct atggactccg ctgaaatgca
     6481 gaacccgctc gtgctatctc ttccttgaga ccggcagcca cgccgcccgc tgcttcggca
     6541 aaatcctctc cattcgaagc gtagagaacc gcccgcgatg cgttgatgag agcgccgcct
     6601 ccggttttcc acgaaccaag tctgacgctc agctccaggt ctcctccctg tgctccaatc
     6661 ccgggtatca gaaacggtga ttccggacag atctcccgga caatcccgat ctgctcgggc
     6721 cgggtgccac cgaccaccag cccggtggat gggttctggg attgcaccag tctggccacc
     6781 ttcacgtaca acggttcccc ctcgcagggc agcatctgaa tctgcgccgc ggacgggttc
     6841 gaagtcagac aaagcacgaa accggctttg cccgggtacc cgaggaatgg ctcgattgcg
     6901 tcccatccga ggtaggggct caacgtgacc gcatctacgc cgagtttctc gaaataggat
     6961 ctcgcgtaca ggtgcgcggt cgaaccgata tcaccccgct tggcatccag gattactggt
     7021 atgtcctccg gaacgctgct gatgacatcc tccagaatag agaaggcatc gcggcccaga
     7081 gcctcgaaaa aggcgatgtt cggtttgaac gcgcacgcgt gcggagcggt cgattcgatg
     7141 atgcgccggc agaaagccgt gacgccggtg ctcgaacggg gtagacacgc cgggagcttc
     7201 tccacaatcg gatccagccc gacgcagagc caggaaccgc gtgtccgggc tgcagctttc
     7261 aacttcgcga gaaaatccac gttacttctc cggggaatgg gcacgttgtg cggatacggc
     7321 atcccgtatt tcctgtgcca gcatggtgta acgtccggtc gctgaaccat tccgaatggc
     7381 aatcgcgagc gccttcttcg aaccgacaag gacaagcagt ctcttcgccc gcgtgaccgc
     7441 ggtgtacaat acattgcgct ggagcatcat atagtgctgc gtcgtgatcg gcatcaccac
     7501 ggcggggaat tcagatccct ggctccggtg aacgctgatc gcgtacgcca ggctcagttc
     7561 gtcgagctcg tcaaactcgt acgtgacgcg aacggtatcc gccgcggaaa ggttcagttc
     7621 gacgctgacc ttggcgttct cggcgtccac gcccacgatc ctgccgatat ctccattgaa
     7681 aaccatcttg gagtaattgt tgcgcgtcac aataaccttg tcacccacgc ggaactccgt
     7741 agtcccgcgt ggcacggagg cacctccggg gttaagtttc tcctgaagca gtttgttcag
     7801 cgcattagcc ccggtctccc cgcggtacat gggtgtcagc acctggattt ccgtgaaccg
     7861 gttgagcttg taccggaagg gaaggcggcg tgtcacgagg tcggcgatcg tagcggctat
     7921 ttcctcggga tcttcccggt agacaaagaa aaaatcgcct tcgcgtgtgt tcggaagctg
     7981 cgggaaaaca ccctgattga tggcatgggc gtttgtcacg atcatactcg cctgtgcctg
     8041 cctgaacacc gtgcgcagcg ccaccgtcgc aacaacctga gaagcgatga tgtcgcggag
     8101 cacctgaccc ggtcccaccg acggcaactg gtccgcatcg ccgacaagga tcaatctgca
     8161 tccggaagga agcgcccgca ccaaatggag gaagagctcc gtgtccacca ttgaggcttc
     8221 gtccacaaga accacatccg cgggaagtgg atgccgctcg tcgtgctgga attcatgccc
     8281 gccgggaaca aaaccaagga gacggtgaat cgtctttgcc tcgtgcccgg tcacttcggc
     8341 gagtcgtttg gcggcccggc ccgtaggcgc acagaggaga agtctctttc gttccgcgac
     8401 cagctcactc acgatccctt gggtggttgt ggttttgccg gttcccggtc cccccgtgat
     8461 gacgaggact ccctcttgca acgatgccat gatcgccgca gcctgttcag gcgcatactg
     8521 cacaccggtg cgcgattcga cgcgggctat caccgacgcc tgaatttcat tgttgcgggg
     8581 tgaaggatgg tgcgtgatca gatcagcaag gcactgtgcg atctggcgtt cgcagaacac
     8641 ataccgcggg agtgagacac gattggcctc aaaaaggatt tcaccggctc gcgccgcgcg
     8701 ctccattatc ggcaaaatcg ccgaaacggg aaccgaaagc tgctgcgctg tttcctgcgt
     8761 caattcgtct gccggaaggt atgtgtggcc ttgttcggtg gagcggttca ggacgtattt
     8821 gattccggct tcgatgcggc ggggatcgtc ctgccctatg ccgacgtgtt ccgcgatctt
     8881 gtcagcggtc ttgaacccga taccggaaat ttcgtccgcg aggcggtacg ggtcagcctt
     8941 gatcactgcg actgattcag cgccgtactt gcggtaaatg cgatgtgcga ctccagtggg
     9001 aatttcgtga tgctgcagga aaagcatcag ttcgcggata tctgcctgtt ctctccatgc
     9061 cgcctgaatc tgtcttacgc gggtggggcc gattccttct atttcgcgga gccggtccgg
     9121 gtgtttcgtg attacgtcca gagtctccgc gccgaatttc cgtactatct tcccagcaaa
     9181 cgcgggcccg attcccttga tcaggccact gcccaggtaa cgtttcagtc cttccgaaga
     9241 cgaaggcaag attgttcgat aactggtgag gcggaactgc tcgccgtatg acgggtgtga
     9301 tttccattcg ccggagactt caaccgtctc cccgattgtg caggcgggta tagtacctac
     9361 tatcgttacc ggcaccttgt caggctcacg cacgagccgt gcgatcgtaa acccggtatc
     9421 atcgttgcgg aaggtgacat gatcgaggac gcctcgcagt gtgtcgttcg aagaaatagg
     9481 tgtttcgggc acgaacgcct ccccttttgc aacaaaaaaa tactcgggat tgccgggcgc
     9541 gggcaacatc ggttgacagt ggtcggagga gcggttatct actgagaagg aaggtatacc
     9601 aggaaaggga ggcggcgttg gcccacgagg ttgttgttgc gaagacggaa gaatggcagg
     9661 acggcagcct cggaatcata gaggtggacg ggcaccgggt cgcgttgtgt cgagttggtg
     9721 acgcattctt cgcatttgac gaccgatgcc cgcacgccgg cagcacgctg ggattgggga
     9781 cgcttcacgc caatgcggtg gaatgcccgc ggcatcaata tctctggcgt gtgactgacg
     9841 gggccccgct gacgacagcg ggcccgctta cgttgtttca ggtgcgcgtg gaaggcgaca
     9901 gcgtcttact cactgtgcct gaccgatggc ccgagcccgc ccactgggca cgccacccca
     9961 aacgggagga actcagacgc cgccacggat tcgaggaagg cgaatgatgg tgcgagttct
    10021 tttgccggaa aaggagcgtt cgtgaaagcg ctggttctgg ccggtggcta cgccacccgc
    10081 ctccacccgg ttacactgga ggcctccaag ccgctcctca aggtcaacgg ccgaccgatc
    10141 gtcgattacg tcctcgacgg catcgaaaca ctgggagatg tcgacacgac gtacgtcgtg
    10201 acgaatgcga agtttttcca ggattacgtg gagtgggcaa aaacccagca gcggccattc
    10261 gatgtggtgc cgttgagcga cgggacgacg tcaaacgcgg accgtctggg cgcaatcgga
    10321 gatattcaat tcgccatcga aacgcagtca gttgacgacg acctgcttat cgttggcggt
    10381 gacaatcttt tcgacttttc gctgcgccct ttgagagatc tccagcgcgc ggcggggcgt
    10441 tcagttctgg ggtgctacga cgtgggctct ctggatctcg tatcgctcta cagcgaagtg
    10501 cacatcgaga aggggttggt ttcttcgttc gttgagaaac cggaaacgcc gacgagccct
    10561 ctgataggca tcctttgcta cgctcttcgc agacaggacg ttccccttgt ccgccgttac
    10621 ctcgaagagg gccgcggtcc ggacaaagcg ggccacctca ttcagtggat gcgtacggtc
    10681 accgaggttg cggcatacgt ttttgaaggc aggtggattg acataggtac cagagcggaa
    10741 ctcgacaaag cggagaaaca gtggtcgtgc cctgcctaag gttaccccgc gcgtgataga
    10801 tggccttccc gttcgctgcc ggtccgaaca ctggaaaact ggtaatacac aaggtcgtgg
    10861 tccgcatata acgtgtgttc aaatccccga acgcacgcta ccatatccgg tggctggaga
    10921 atctcttcca ccatcctgcc gtccttgccg cgcatcgtaa cggtcgggtg tccttccaca
    10981 aaccaccagt attcgtcgaa atcgtggaag tgcagttcca ccggagttct ggccggcaag
    11041 cggtactggt ggaaggtcgc tgggtctacg gggttcagca ggtccgccag ttgtccgcaa
    11101 atcctggcgc cctgactgaa acggacgatg cagatgcccg ggtctgttcc ctgcgttatt
    11161 tccaccatgg tttcgccttc tttcggattt cccagttcgc tggcataagc ctgcgcttcc
    11221 gtcataactt gccctgtgtc cgcctctgag tgtggagtat attggatacg cgggataacg
    11281 gcaaaccccg cgattcacgc ccgggacagg gaggttttta tgagtgaccg tttggcgatt
    11341 gatggaggca cgcctgttcg taccgcacct cttccgccct cgtggttggg cgcatacgag
    11401 atgggagtgg aagaagaaga agcggtgctc aaagtgctgc gggaaaagag ggtttttcgg
    11461 tacatcgcga aagaggagtc ggaaactgcg agactcgaaa aggccgttgc cgagttcacg
    11521 ggacgggatt acgccctcgc cgtcaacagc gggactgcgg cgctgatctg tgctctggtc
    11581 gggcttgata tcggaccggg ggacgaggtc atcgtgcccg gttatacgta tatcgccacc
    11641 gccgccgcag tggttacggt gggagcggtt ccggtgatcg cggaagtgga caattctttg
    11701 acgctggatc cggaggatct tgagcgaaag attaccgggc ttaccaaagc tgtgattcct
    11761 gtccacatgc gcggaacacc gtgtcgcatg gacgagatct gcgcgattgc gaagaaacat
    11821 ggaatcaaag tcatcgaaga cacggcacag gcgaacggcg gcaactacaa gggcaaaact
    11881 ctgggcggta tcggggacgt cgggtgctac agctatcaga tgtcgaaaac cataaccgca
    11941 ggcgaaggcg gcatggtggt taccggcgac cgtctggtgt tcgagagaat tctcatggcg
    12001 cacgactccg ctttcgggtt ctggaagccg gatgagacgg ccataaggcc gattcctgga
    12061 gaaggattcc gcatcagcga agtgagcgga gcgatcgcgc tggttcaaat ggggaggctc
    12121 cgaggcattg tcgaacgcct gcacaacgcc aagtaccgaa tcgtgaacca gattcgcggg
    12181 cttgccggga ttactcttca ggacgtgccc gacgaatcgg gtgacgcgtc ggtctgcctg
    12241 atgttttttg cgccggatgc ggaaatcgct aatacatacg caaaggcact gaatgcggaa
    12301 gggataccgt gcggaacaat gttcagcaag gagattccgg accggcacat ttatccaaac
    12361 tggacgtacg ttctggaaaa gcgcggacga tcctccaact ggagtccgtg gcatccttcc
    12421 gtttacaagg ggagcgtgga gtacagcaag gatatgtgtc ccaaaacgct ggactatctc
    12481 gggcgaacga tccacatcgg tttgagtccc agactcaccg cggcggacag cgatgacatt
    12541 gcccgcgcaa tcagaaaggt ggtgaccgcg ctcgcgcggt gattaccgtg aaagcgtggc
    12601 ggaggcacat gcaggaacgt gcctccgccg cgaagccgtt ccggtcatga acgagggagg
    12661 ggagctggct tatgaattac ccgttctggg acgtcccgct gctcggcggg ggactcgtca
    12721 tcgcattgat cgctattccc cacgtgttcg tttcgcattt tgccatcgga ggcgggattt
    12781 ttcttgcctg gaccgagcgg atcggaattc ggaaggggaa acccgaatac attgcatttg
    12841 cgaaacggaa cacgctgttt ttcgtcctga tgacactggt gttcggagca gtgacgggtg
    12901 tcggcatctg gttttccatc ggtcttgtcc accctgacgc cacctccgcg ttgatccaca
    12961 atttcgtgtt cggctgggca atggagtggg tgctgttcct cgtggagatt gccgcagcac
    13021 tgctgtacta ctacggctgg gatcgcgtgg accagaaaac gcatcagttc atcggctggg
    13081 cgtacgccgg ggcggcgtgg ggcagcctcg tcgttatcaa tggcattctg gctttcatgc
    13141 tgaccccggg aagatgggtg gaaacgggag gcttctggac aggtttcttc aacccgtcgt
    13201 atttcccgag cgtgctcatc cggacctgcg tgtgccttgc tcttgccggg ttgtatgctc
    13261 tcctgatggc gtccagagaa cctgccggcg agactcatga caaactcgtc cgatacgcct
    13321 ccacgtggtt gcttcctgcg ttcattggcc tgccgatatt cgcggcgtgg tttgttttta
    13381 cgttgccttc gctcgcgcag gacatgttca tggggcgtgc cgtggtgctc acgattttcg
    13441 cggcgttgag cctgggcatc tcggcgatca ttttcgcgtt cgcgtacctg attgcctacc
    13501 ggaatccaca cagcgtttcc acgccggttg cgcttctgtt tctcgtgctg ggccttgcgg
    13561 ttaccggtgt gaccgagttt gggcgggaag cagcgcgcaa gccgtacatc atctacgact
    13621 acatgtattc aaacgcggtg ttgaaacggg acgttgccag gcttcagcag gagggcgtgc
    13681 tgcggaacgc gaaatgggcg ctggtgagca aggcgactcc tcagaacgcg ttgattgctg
    13741 gccgggagat attcacgctc gcgtgcgccc attgccacac cgtggacggg tacaacggcg
    13801 tgcggctgct cgtgaaaggc tggccggaac ccttcatcga gcaccagttg acgtatctgg
    13861 accagctcaa agggttcatg cctccgtttg tggggaatcc tgaggaacgg cgggcgctgg
    13921 cgaagtggct cgcttcgctg aacccaagcg ctctgccggc gcgaaccgcc accgcggcaa
    13981 cggacagcct gagtgcggat cctctggccc gcggcgaagc ggcgtattcg gctcactgcg
    14041 ccaattgcca tacgattgac gggcgcaacg ccatccgcca gcatgtgaaa gggtggaccc
    14101 agtccattgc gtacgaacgg ctgggcaggc tggagagtct gcgcgggtac atgccacctt
    14161 acgaaggacc ggaagcagaa cggcaggctc ttgcagcgtg gcttgcctca atcggaaacg
    14221 acgctcctgc ggcgggaggt gcgcgatgaa cgaagcgatc atcccttcaa tcgatcccat
    14281 tgctctgccg gcaccggtgt ggttgatgca gttcctcagc gggctgactt tcatttttca
    14341 cgtcatcccc atgaacgtcg tgctgggagg cggcgtggtg gcgctgttcc tggcggtttg
    14401 gggaacgcgc gggtctcgaa agaaccacgt tcggctcgcc agacgaatga cgtcggtcct
    14461 ccctgcggcc atcgcaatga cgataactct gggcgtgccg cctctgcttt tccttcaggc
    14521 gatgtacggc ccgctgtttt acacatcgtc ggttctcatg gcgtggccct ggctcagtgt
    14581 gattgcgatt ctgcttgtcg ggtactacgg gcattactac ttctcgctct caaaggattt
    14641 cgcaaatacg cggacgctgg tcgtcggcgc ggtcgccgtc gtggcgtttc tggtgattgc
    14701 gtttctgtat gtgagcaata tgacattgat ggttatgccc gagcgtttcc tggggatcca
    14761 acgttcgtgg acgagcggtt ttctgccgtt cacgggcgat tcgattttcg cgccgcgttt
    14821 tctccacttc attaccggcg cggtttcggt cggtggtctt cttgcggtgt ttgtcggatt
    14881 gctggaacgg tcggatgctg aattcaaagc ggtcgcggta cgggcgggcg ggttcacgtt
    14941 tgtcggcgcc acgattgttc agatcgcgac aggcatctgg ttcctgattg cgctccgccc
    15001 gcgcgtgatg atgctgttca tgggacagga ccacgtggcc acagccctct tcggtgttgc
    15061 cgtcctgctc gcgcttgtgg cggttgccct gattctggtt gccgttattg gcaaaaaacg
    15121 accgtggatg acgatcgctg gaacagcgtg catcggcctg acgatcatta tcatgacggt
    15181 gatgcgctcc atcgttcgcg aggcgtacct tggcacgcgc ttcgtcgtgg ccgaacaacc
    15241 cgtgaacccg gcatgggatg tggtggttct ttttcttgtg ctgctggttg ccggagctgc
    15301 gcttgtgggc tggatgattc acgcggtggt tacgcgaaga ggtctcgcgg cgtcctgacg
    15361 aacttctatt ctgcggaaaa ggaaggcgaa cacggaggtc gtgttcgcct tctcgttttg
    15421 tggcgattgc tcgatcaaac cgggcacatg gtactgaatg tttcacgtgc ctcggtgagg
    15481 gtggtcgctt tgatcgtgaa acgtggcgcc aacgtccgca ggaacgccag ggtatcgcgc
    15541 agggtctgac tctgcgggat ggacgcgtca tcatacgcga aggtgttgtg cgtgatgaaa
    15601 cacagcgatt tgaacgcgac gcggttggtt tccatgtcgg cgaggaccgc ctcgatgatc
    15661 ggctgctgcc agtctttcaa tggcccccat tcgatgcgca gttcgtgcgc aaggccgtcg
    15721 ggactgcgct ccgtcggatg agtgctcatg ggcacttcga aaaagggaag atcgcccgga
    15781 acaaggcggt tcgagcggtg cgcccaatgc gcatatcggt atgctgtttc ccaccgggcc
    15841 gcgtatttgg gaacctcccg cccgggacag gagactgagc ccgcaagaaa cctcaactcc
    15901 gacagaaccc ggaaagtgtc atcggttgca gagtcattgc ccgcacgaaa cgaacggggc
    15961 gcccatccga tagcatcagc gaacacggtc atcgcttcgc tgatgatttt cctctgcata
    16021 tcggcgtcgt actcgccgag gtatctgccg tatttgtggt ccccgaaact ctggggatgc
    16081 acgtggagac ccagttcgac cccctcctcc gcgtagtccc ggaacatcgc ggcgtggcgg
    16141 gccgcgcact ccggcgtaag gaacagcgta ggaaacgcct ccgctgccag aagcgtatcg
    16201 cagtaacccc ggattgcccg ctcactcagc tcccatgtcg caggaccgcc ggggggcgat
    16261 tccgccgcga tgcgttcgca gtccatggtc caggcaatgg agagaggttc catcagtttc
    16321 ccttcgtgtc gggcaggcgt cccgcgcggt accgtcgcca gagttcgtca agagcaaccg
    16381 cggcccgttc ggcgatatga aaatcgaaaa cgtccgtaag agcggttgtt tccgggttga
    16441 tttccaccgt cggagtcccg cgcgcttttg cccgcatcac cggttgcgcg atgtacggga
    16501 atacgctcgt ggtgccgacg gaaaagacga tatcgaaacc acgttccagt tcgtggagca
    16561 ggcgttcgac ctcagaagcg ggaagctgct cgccgaacag gacgactccc ggtcgaatca
    16621 ccgcgccgca tgaggggcag gatggcggca gttccaggcc ggaatagtcg ggcacacgcg
    16681 tgcggtacga acagcgcgtg cagcgcaggt cgtgcaggtt gccgtggatt ggaatcacgt
    16741 tggtgctgcc cgccgcggcg tggaatccgt ccacgttctg cgtcaggacg cacacgcgat
    16801 cgaacagcgg ttccatctcc gcgatgactt cgtgcgcgcg gttgggggtg cggccacgcg
    16861 aggcagactc gatcctcgcg atgtacttcc atgtcacctc agggcgtttt cgaaatgtgt
    16921 cgccggaaag cgctgattcg atgggtattc cgtcttcggc ggtacacccg tcgtagattc
    16981 ctccgcggcc acgatatgtt ggcaggcctg agtccgcgct tattccggct cccgtgatga
    17041 agagaacgga gcgggaggtt cgcagcaggc gagccacccg ttcaatgtcg tccagaacgg
    17101 cttggtgttc tggtaacccg ttgtcgtaat gctgtcgttt gtgttcttcg gttctctgag
    17161 ccactatttc cagacctcgt caataacctt tcttgtcaga gcaacccagc gtcgcacgcg
    17221 cctcggatca ctctgaaccg tttcgacgtc cttgagcgtg atgtccacgt ggcaatcgcg
    17281 gcacgcttcg aggtccttcg tcaggatggc gcgcacccgg tcttccgcaa gtccgtatcc
    17341 caccatgtcg ctggggctgg ggcggtaact cacgacgtag tcccgaccaa tctgctccgc
    17401 gcaggccttc acatccgcaa aaggggaaac cgcgatgcgg cgcaggttcg gaatctggcg
    17461 gagcatgtcg attttgtggg taaggtcctc gcagcagccg tacgcgacca gcccgaaatg
    17521 tttgaggatg ggcaactggt actgcaacaa aaactcgtcg tgcatcgccg gggacaccgc
    17581 agtgaactct tgcgccgcca tgtatcccca gaggcgagaa cgcttcacac cgttggcgtt
    17641 cgccgcgggg tcgggcaatt cctcggcgta gggcatcgcc tggttctgat gcgccgaaag
    17701 gccccagtct cccgctgctt ccgcctcctc atgcgttttc aggattccat ccgacatgaa
    17761 ctgcacgagg cggtgaaggc cttcggggtt atctaccatg tccaccatga aatgctcgat
    17821 gccgcggaga tacccgaggt cggtggaaat gtcgcccgac cacatccggt acgccggtgc
    17881 tctatccacg tttatggtga ttatgtcgcc aattgcgtcc gcgagttgct cataccggcg
    17941 tgccgtgcac tcttcatcga tggaatggac cggtatacgg agccgggata cgtcttcgag
    18001 actcttcagc gggtactcca ccttgaacga acccctcggt tcgttcgaga aatgtcgttc
    18061 ccccgacacc ccccagccgg tgcaccggtg acacgcagtg agagtaaccc atggttcgaa
    18121 cacggaatca tcgtccagcg tgctccagaa aagcttttgg cggaatacgt cctcgaaatg
    18181 ccgcaggaaa gggtcgctga tgacgcaccg ggagtcgggc atctcgctcc acgcgaatgc
    18241 ccggacgtat atcaacgggc gcgtgggctt gaggctgttg tgtgttctcc acaacgcgcg
    18301 ccggatatcc tgttccggcc gtgagcacac ctccacgtag cgccgtacga gatcccggag
    18361 aatcgccact tcgtggttca cgtgagaccc ctcagggatg gggacgtgtt tcttcccgct
    18421 cgttccgcgc cgagccctcc tggtgggttc acatcaatcc gtggcggctg agacggcctt
    18481 gagaagattg agatgagccg ccgcgaaacg ctgcgggttc tcgtccgcat ccctgaaccc
    18541 ccggaagcac gaagacagaa tcttcagggc ggactcaggg tcgacttcct tcagttgccg
    18601 taacagctcg tagtcctcaa ttcccgcggc ctgcgcttcg agtcgtacgc tgccccaggg
    18661 gccgtcgtcc ccgggataca cgttgtgagt atcacccggc ggaaggttgt tcccgccgcc
    18721 gtgatccggg cagttgcact cgaagggatt ttgatcgtgg cgataatggt tgaggcccca
    18781 gtggaggaac cctgggagat cgtatttgaa acaaccccac tgaatgtagc gcgtgcggag
    18841 aagcggcatg tcgagcagtc ggttcatgta gtagccgccg gggaagcaac agatgtagca
    18901 ccatatcgtg tcgccgtgat gtcggaagaa ctcgaactcg tcgcggtgtt tctcgtagta
    18961 gtcatccttg ggcacccaga tatccacggc gcccgcgagt tcagaaagct ccacggcgtc
    19021 gatgatcggc gtacccggca tgaatttccg tactatcccg gagatgaccc ggtaatccgc
    19081 catggacttg tcggtgggct cgtcgcccac atgctggacg gcaatatcaa gccagccgtt
    19141 gcggcagagg aaatcccgcc aggcgggaag gtactgcgcg aggaaattgt agccttcgcg
    19201 ggagacggcg ttgactttgt ggttggtgga aagcacgaag ttgggcgcgt ggaattccgt
    19261 gcggccggcg acatgtcctc cctcgattcc cgtgaagccc agtttcaaat aggtgcggat
    19321 caagcgctct gctttcgcga aatcgaacga aaagaggcct tcaggtccct ctttgatacc
    19381 gatgtacccc atcggaatcc agaaatgcgt ctggcgcccc cgacgcatca gctcgccgta
    19441 ccgacgaatc atccgccagt gggcttcggt ccacggcgtg attccatggc gggatgccat
    19501 ggctccaaga ttgaaccaat tggtgaggct gaacgtgccg gggtcagtta ccgtggccgc
    19561 gtgaaccgtc agtttgacgg gcagagtaag cgactcgttc ccgatcgtga cgcgaatcgt
    19621 gctcgtgtac gtggcggcgt cggcgtcctc gggaatgcgc cactcgaaga acagcgcttc
    19681 ggagcccatc cttcccttgg tgcccgggag aaatggtttc atggcgtcgt acacacggaa
    19741 cggcgcgagg cgtgtggtat acccctccgc ggattcgcct tctttcgcga tgaaacctat
    19801 cggcccggtg ttcttttcca ccatgacatc gacgagctgg aagatctcgg gcgaggggaa
    19861 gtccgctccc tgcgacgcaa ggctcaccct caattcgccg tttgccggtg tcggtttgaa
    19921 gagaacctga aaaccggttc gcgttccccg tgctgcgtgc agaacgatgg gtttgggccg
    19981 cgaaaccccg gccgccatgt ccgggtagag ccactcgttc atactgacaa ctctgcactt
    20041 catcgtctgc ctcgtctgtt ggaagtcaat accggcgtca cgtctcaaga cgaaatcgag
    20101 tcaagccatt gtagctcgga tgcggtcaga gggcccctgt gaacgctttc cacgttggcc
    20161 gtgacccatt ctacacgccg catcgccgtg atcacacgat caacgttcgg gtgaaagagg
    20221 gagtacctca acatgtggtc cgccagccgg cgccgaagat cagactcact gccccgccag
    20281 gacttgcggg cagccgcgac gagcctgtcg aggtgccatc cccgaacaaa aggagacgcg
    20341 gcaagagtgt gccagccaag gcgtttcgcc gccgcaaatg cgggagccgc gtccggagtc
    20401 gca
//
LOCUS       DAOSOO010000034        20107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2680_length_20107_cov_5.385897, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000034 DAOSOO010000000
VERSION     DAOSOO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 20107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20107
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_20107_cov_5.385897"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLT86_04150"
     CDS             complement(<1..695)
                     /locus_tag="PLT86_04150"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOT35842.1"
                     /translation="MKERVLDGQMSRRTFLGVGATGLAATALHGLLPSRSRADSTPRT
                     RTTAPDGRPCAVVRTAEFFGDEEMVLTFPHGWQVQTCYMDGHFAPPLTDDEIRAALRS
                     TVQTGRIAELAQGKRSVVITFDDCARPTPTGRVVPFVIEELHAAGIGDDQIYFHGSFG
                     THAAMYQQDFTAKLGADIVAKYPVWNHDCFNGMTQVGTTSRGMPVKLSKRFVDADFKI
                     CISGVKPHGMAGYG"
     gene            1297..3756
                     /locus_tag="PLT86_04155"
     CDS             1297..3756
                     /locus_tag="PLT86_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT35843.1"
                     /translation="MLRKALPPKYAAWLSEQNIPESDILLCTDNDITFEGEYAERWVV
                     VTRDAVIMLRERDGQVQAARSFKMADIESARIDPRVGSGFLQVKVHGHWAEVARFSNA
                     RAIWFERIAAKITRFKETGEIILTEEDEKEQNRCPTCGLLLRSSTDICPRCINKNRVF
                     GRLLRMMQPYWPWALGTLLLVFTGIGLDLVPPQLSRILMDDIFVHDPARPYLMPEWAL
                     RLMNITPGTQMTPSEKVTLLFFVVFLGMAGVQLLRIGLNIVIGRLSVTVGTKITYDLR
                     HKLFSRLSELSVRYYDRHQVGILMQRVTGDTESLHGFVSQSTQGFLVNILLPVFIGAA
                     LFKMNPTLTLYVFIPAPFVIISTYYFWEFVYPNYYKYWDSHSKMNAMLNSVLSGVRVV
                     KAFAQEEREISRFNHLSGNVQASRQRVDKATGTFYPIVGFIFGLGGLIVWYVGGRYVL
                     EGKGMTLGTLTAFTSYLGMFYGPLSSLTYISSWLTGFMTSAQRVFEVLDTEPELQDTD
                     SALHLHICRGEIEFKNVTFGYDRYNPVIRDVTAHIKPGEMIGIVGRSGSGKTTFVNLL
                     CRFYEVDQGEISIDGISLRELHKDDIRRNIGLVLQEPFLFRGTIRENIAYGRPDADIE
                     NVMKAAKAANAHDFIMRLADGYDTKLGEYGSGLSGGERQRISIARALLYDPAILILDE
                     ATSSVDTESEKQIQEALAVATKGRTTIAIAHRLSTLRNSNRIIVMDDGRIIEMGTHDE
                     LIKKEGTYYRLVKIQTQLTSEPTVDRLELEKKEQEAAVSDESSEKETEEETEKDSEEK
                     TDAESTEPSEDESSKDSEQTS"
     gene            3781..4326
                     /locus_tag="PLT86_04160"
     CDS             3781..4326
                     /locus_tag="PLT86_04160"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOT35844.1"
                     /translation="MEESTTQKPTESGYKGLVFLDPKDITIHESELAGMNVTVGGETF
                     EEVRASLALPITEPDRYVSLRIGASKGEEQEIGMIRDVNELSPENQVLVRRELRKRYF
                     LHVIYKILSLKEKFGFLYFEAETSKGLRKFAVRWEYNRVQEYSEFGRIIIDTDDNRYI
                     IPDLRKLSPEEYKNFTRYIYW"
     gene            complement(4418..6916)
                     /locus_tag="PLT86_04165"
     CDS             complement(4418..6916)
                     /locus_tag="PLT86_04165"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT35845.1"
                     /translation="MGKKRDLGAVLLCKPVSLESLFNWPKQVPDDEWWSNIATRVRAF
                     PEGKQVSWGIPFRLGKGKNKRVIMLARDTQDVTVSLPADNGSSISYLCLLHAWFEKVP
                     DDKTLPPQEGLPVAEYVITYDDESAYVQTVRARFEVPMASSPGPPWLAVQFDAWTAID
                     PTKPTTDMGWGVAQFGFGKEHVRQQPAIVLYALPNPHPERKPASLTIRGLVDAPFVVA
                     GLTAYFGEQHPLRYLPRRHYRVTFDDMPRHVTRAELDLGEITRIERTTGPRNEEWLGA
                     PHAGVSGAREPDTGAEDLIEAAGAPDATMSLRLEGKKKAYSFRLGEVFEKGVSESGKA
                     KLEVIGRQRQWLNVRIIDSSTGRPTPVRVHFSGPHGEYLPPYGHHSQVNTNWFEDYGA
                     DVRVRDMSYAYVPGEFRTELPVGDVFVEINKGFEYQPTRARVTVQPGQKELELRVNRW
                     KDLRSAGWVTADTHVHFLSPHTAWLEAQAEGVNVVNLLASQWGRLFTNVGDYIGRVGV
                     AENDTIVYVGTENRNHMLGHMSMLGTQGMPVVPMCAGGPTEAWIGDPDYRTLAEWAME
                     NRQKGGVVIRPHFPYCGCTEDPVPIIAGLVDALETGSLQGGNFPLQEWYRYLNCGYRV
                     AVAGGTDKMSASCPLGWIRTYARVDTNEPFTYDAWARAVRAGRTISTTGPLIDLSVDG
                     HGVGDVINVRTRAGTYEVDAIAESAWSLGKIEVVCNGRVVASEESAAGAKTLRCKANV
                     VLSGSGWIAARCVGHANQPGYLVAHTSPVYIAARGSVLFDGPAAEHMLQLVNGGIEYL
                     RTLSTPYDDASRERMVRLYEDTKKTLTARIAAGK"
     gene            7117..8496
                     /locus_tag="PLT86_04170"
     CDS             7117..8496
                     /locus_tag="PLT86_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT35846.1"
                     /translation="MIRTDVSITAEALLPQIEAAWGLSASKIDLLTRNRSATDGSPVF
                     TVRGQYTSKGWTEWTQGFEFGSALLQFDATDERRFLDFGRSKTRAVMASHITHMGVHD
                     HGFNNISTYGNLRRLILEGRFASDAAELEFCELALKCSGAVQARRWTPTAEGGGFIYS
                     FNGPHSLFADTMRSLRSLSLAHRLGHALLEEQDARVSLLSRMVAHAETTARYAVFYGE
                     GRDAYDVRGRVAHESIFNINNGVYRCPNSQQGYSAFSTWTRGLAWVMLGFVEELEFLE
                     TVPDGELEPLGGRTRVEAMMRRAAEATCDFYCQNSPTDGIPYWDTGAPGLAALGYYLD
                     RPADPFNDYEPVDSSAAAIAVQGLLRLGKWLSDHGNSETGARYWQTGLTVLHNLLAEP
                     YLSTDEKHQGLLLHSVYHRPNGWDYVPEGRRVPCGESSMWGDYHLREALLFVQRVALR
                     QPYLTFYTP"
     gene            8511..9971
                     /locus_tag="PLT86_04175"
     CDS             8511..9971
                     /locus_tag="PLT86_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT35847.1"
                     /translation="MTEIRPIKGIKTVEDFRAYVEARGLDLHCEDSIAPAASSPLCEP
                     LHVYGRTIGNRWAVHPMEGWDGTTDGGATELVIRRWRRFGESGAKLIWGCEAMAIRPD
                     ARANPNQLIISSQTESDLARLRGVLLHAHRERFGTTDDMMIGFQLTHSGRFCKPWDKG
                     KFESRVAYRHPILDRKFGVTSDAQIFRDDEIGPLVEDYIKAAKIARNVGADFVDIKHC
                     HGYLLHEFLGAKTRNGPYGGSFENRTRLLREIAAGIRAEVPGMLLGVRLSAFDTVPYK
                     PDPEKSAGGVLGPGIPEDYSGCLPYVYGFGVNETDPVQYDLTETCLFLELLRELDIRL
                     VNITGGSPYYNPHIQRPAIRPPSDGYAPPEDPLVGVARHINVVKYLKARFPDLVLVGT
                     GYTYLQEYLPHVAQYYLRHQHVDSVGIGRLVLTYPHFFVDATTKGVLDARIICRTLSD
                     CTTGPRKGLPSGCYPLDEFYKTSEYGARLRELKKAR"
     gene            9986..10969
                     /locus_tag="PLT86_04180"
     CDS             9986..10969
                     /locus_tag="PLT86_04180"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT35848.1"
                     /translation="MDSSRELTIQDALNEALRTEMRRDERVFCMGEDIAIYGGAYGVT
                     KGLLDEFGAERVRDTAISEAAIIGAGVGAAMTGMRPVVELMYVDFAGIAMDQIYNQAA
                     KIRYMFGGKAKVPLVIRTQGGTGKQIAAQHSNSLEAWFLHIPGLKTVMPSTPYDAKGL
                     LISAIRDDNPVMFIEHKAIYNEKGPCPEGDYTVPIGKAAIRRPGTDATIVSYSRMALR
                     SMSAAETLAGEGISCEVIDLRTISPWDTHAVVESVAKTHRVVIVEEGTRTGGVGAEIS
                     ARIMELAFDELDAPVERVAGVDTVIPASATLEAAAIPNVESIIAAVRRTLQ"
     gene            complement(11028..11546)
                     /gene="rplI"
                     /locus_tag="PLT86_04185"
     CDS             complement(11028..11546)
                     /gene="rplI"
                     /locus_tag="PLT86_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT35849.1"
                     /translation="MKVILRDNVETLGRAGDVVSVKDGYARNYLIPRSLAYPATERNL
                     RVWEDEKRKFLVRVSKETQEAERVKAALEPVHLVIPMQAGEEGRLFGSVTNRDLAEAL
                     KTHGFDVDHRHIVIEEPVRALGAFEFFVKLPHGVTATMKGEVVDAAGQKAPETVEDTE
                     TKEPEGTIEQSE"
     gene            complement(11572..11805)
                     /gene="rpsR"
                     /locus_tag="PLT86_04190"
     CDS             complement(11572..11805)
                     /gene="rpsR"
                     /locus_tag="PLT86_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT35850.1"
                     /translation="MPREKKIRVKVCKFCEAKITHIDYKDEKLLRRFLSDRGKIIPRR
                     MSGVCERHQRMLTTAVKRARQIALLPYVSEMIK"
     gene            complement(11810..12259)
                     /gene="rpsF"
                     /locus_tag="PLT86_04195"
     CDS             complement(11810..12259)
                     /gene="rpsF"
                     /locus_tag="PLT86_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT35851.1"
                     /translation="MPLYETTLVIDSLLEETAITEKVQKYVDLVTKAGAVDLQVDRRG
                     VRKLAYDIKGKDGQWRTQADYTFLFYEAPGTAVNILEKQLLLDEDMLRTMTVRYDRMP
                     PATPADLNAIEAVDSSRDDDEERGRGDRERVKDTGVDVDALDEEEEG"
     gene            complement(12499..15126)
                     /locus_tag="PLT86_04200"
     CDS             complement(12499..15126)
                     /locus_tag="PLT86_04200"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT35852.1"
                     /translation="MSESMLLSIVLGISVFGLIVAWALAKWVLKKDVGSQAMQDISNA
                     IKEGAEAFLRRQFKTIIWLAAAFAVLLFIGYGFLRNHMPTDPVTTATGLAFWITLSFV
                     LGALCSLFAGYIGMWVSIRANIRTATGAMKNLNEAVQIALRGGAVSGLMVVSMSLLGI
                     AGLYTAVKLTTAVDPTKIPLLIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVE
                     AGIPEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILAASMAKAVPDAS
                     PLWILGVMMFPLIARAFGIIASIVGIMMVKVKEHEDPMSSLNRGYYWAVVLAMIGFGI
                     ASYWLLNHPSAPSAWWHFFLCGIIGVITSIAFVYITQYYTESKYRPVKSIADASKTGP
                     ATNIISGIAVGLECTAMPVIVISIALLASYYLGASSGFAHAGLFGTAVATMGMLATAA
                     YILAMDTFGPITDNAGGIVEMSQQPEEVREKTDRLDAVGNTTKALTKGYAIGSAALAA
                     FLLFSAYIDEVNIYLQGKGMILSTVDIAKPEVFVGALLGAMLVFLFSAFAIRAVGHAA
                     QYVIQDVRDQFKEKPGILAGTEKPDYGRCVDIVTKGALREMVLPGLLAVLMPIVVGVV
                     FRFMVFGGDAWRPLGIVSFKADIPAVEQVAGAEALPEAAVAEQNTAAPAEGEMMTEAP
                     AEEVAPAVACAAESVMTYGAANTFAFAADTAYLSRPLVDEVWAITKKKYVDEDSLIAI
                     GQSRIRATSVEVVRSYNADSALSIQPASVVVTGVKSASAVVKSPVGAETVAALLMVGT
                     IAGILMALFLNNGGGAWDNAKKHIEAGYLGGKGSDPHKAAVVGDTVGDPFKDTAGPSL
                     HVLIKLLSTITLVLAPLFI"
     gene            complement(15277..15927)
                     /gene="pth"
                     /locus_tag="PLT86_04205"
     CDS             complement(15277..15927)
                     /gene="pth"
                     /locus_tag="PLT86_04205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT35853.1"
                     /translation="MIGLGNPGPSYARTRHNVGWFVLDSLIPLDRWVRGDGPFLFAGY
                     PAVGSECVLVKPLTFMNLSGEAAVAVCRQFTVGPADLLVLLDDVNLPIGDLRLRRSGS
                     AGGHNGMASIGEALQTQDVPRLRIGIGRPSEPGGLVDYVLSPFSEEEEPVVRQAAIEA
                     GGIVRAFCSGGFEGAAELYSRWKAGSRSEPVAGWEKTDKQSAGMNEEASGDPAKTE"
     gene            complement(15942..16586)
                     /locus_tag="PLT86_04210"
     CDS             complement(15942..16586)
                     /locus_tag="PLT86_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT35854.1"
                     /translation="MNKFSLTVQARTRHGKQYAKQARRKGLVPATLYGAGANPVSVQA
                     DARTLTRVFHQARTTAIIDVTVEGGETCQALFREPVFHPLNDALLHVDLMRVSSTSMV
                     RVEVPVEIVGSSKGVLAGGILDHVLHALEIECQALAIPERIPVDVTQLEIGDHITVGE
                     LKLEGLRILTDTDQTIVTISAPRVEEEAPAEEEPTAAEPELVKRKKATEEEEES"
     gene            complement(16646..16720)
                     /locus_tag="PLT86_04215"
     tRNA            complement(16646..16720)
                     /locus_tag="PLT86_04215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16686..16688),aa:Gln,seq:ttg)
     gene            16931..17560
                     /gene="hisH"
                     /locus_tag="PLT86_04220"
     CDS             16931..17560
                     /gene="hisH"
                     /locus_tag="PLT86_04220"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT35855.1"
                     /translation="MIAILDYEGGNLASVLRALDHTGFDACITWDFETIRRADHVILP
                     GVGAAKATMHHLRTRGLDKLIREEVIGGGIPFLGICIGVQVIFGSSEEEAAECLGILP
                     GSVRRFPSVCGGEQIKVPQIGWNQVRFSKPHPIFAGVEDGTSFYFVNSYYPVPASEEL
                     VYAKTTYGVEFASVVGKGNLVATQFHLEKSGVPGLRMLENFCRWDGSLS"
     gene            17590..18642
                     /locus_tag="PLT86_04225"
     CDS             17590..18642
                     /locus_tag="PLT86_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT35856.1"
                     /translation="MEKKTCLMLGAGGMAGWWIRNAFPRFVDRMTVVGLVDIRENVLH
                     ESGNLLGIPESARFTDMKEAFAKTEADFCVIVTPPWVHEQAVMLAVGKRMAILSEKPI
                     ADSWKACTAIYRAVAAAGLKMEVIQNYRYEPTMLTVRHILRSGRLGRVNYIVGRFADD
                     YRRYGSWGQFRHEIPHSLLVEGGVHHLDMLRNLSGGDTALISGSEWNPPWSSFKGESN
                     ALFTLTMTNSVRAQYEGSCNEAGVVNGWHREYYRVECEYGSVTVDSDLVVRIWERSEG
                     RSCKMTEVPLLNPTYAGHLWQLDEFLSWLDGGREPDTVLSDNMQSAATMFAAIVASDD
                     AATVDVQKMVRAAMQA"
     gene            18732..>20107
                     /locus_tag="PLT86_04230"
     CDS             18732..>20107
                     /locus_tag="PLT86_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35857.1"
                     /translation="MATFVKTIPLLDSRIVAQAFALRRVVQHPVLVSEDVVPGDTQAY
                     GSVLRDRKGVWRMWYLGPPIYCEYYAESEDGLNWVRPHLDFVAPEVRSEVEGPNAFLC
                     RNQKDVKGAWLVGSYGPEGFCVLDAEKTPHPAAKARFTALYLARTETESGMYIAYSDD
                     GTRWVADSGMPVIPGWRDTSSVFFFDKRKQKYVWYGRPEAYAAPSMHANRLLAYRESD
                     DLTHWTPDRTVLDTDDADADPHHLLDERALHGGQQTIATAEQRARVWAELTEGQTPDT
                     DRPLVRGRNRQWYGITAFPYADLYVGIVWMYDLPTGSMWTELLHSYDGVDWRREAIRV
                     PFLEAIPGTCMCTMASPPVDLAEETRLYYSVSNQSHHRVPNPSVKKGIRIAALRRDRW
                     VGYVAGDVQGELLTQVMEKPRDLTLNAQTEGDGWVRVEVDDADGRAIDGFSADECVPF
                     SGDDLGWRP"
BASE COUNT         4149 a         5801 c         6002 g         4155 t
ORIGIN      
        1 ccgtacccgg ccattccgtg cggcttcacc ccggagatgc agatcttgaa atccgcatcg
       61 acgaaccgtt tgctcaattt gaccggcatt ccgcggctgg tggttcctac ctgcgtcata
      121 ccgttgaagc aatcgtggtt ccagacggga tattttgcca cgatatccgc gccgagtttg
      181 gcagtgaaat cctgctgata cattgccgca tgggtgccga aggagccgtg gaaatagatc
      241 tggtcgtcgc caattccggc cgcatggagt tcttcgatga cgaacggaac cacgcgaccg
      301 gtgggcgtcg gccgggcgca gtcatcgaag gtgattacga cagaacgctt cccctgcgca
      361 agttctgcga ttcgaccggt ttgaacggtg gatctcaacg ccgctctgat ttcgtcgtcg
      421 gtcagcggcg gcgcgaaatg cccgtccata taacaggttt gcacctgcca cccgtgcggg
      481 aaggtgagga ccatttcctc gtcgccgaaa aactccgcag tgcgcacgac cgcgcagggg
      541 cggccgtccg gtgcggtggt cctcgtccgg ggtgtgctgt ccgcccggct tctggaaggc
      601 aggagtccgt ggagcgccgt agctgccaac ccggtggctc caaccccgag gaatgtgcgc
      661 ctgctcatct ggccgtcaag gactcgttcc ttcatagttt tccccttcct gatccggcgg
      721 atggtacttt gcgccttcgc atgcgcgacg tggttgagga agaatatgga taatgcaagg
      781 aagcgcggca acatccccgt gcggcggcct tgtcaaaatg ccatgtatcg cgtagctttg
      841 aggatactag aacgcgctgc aatgaaggca gctgaggttc aggcgcgctt actttcctta
      901 agaagcgctc acgcgtccca tgaagtgaaa agaatcgctg ggagacttgt tcggaggcgc
      961 cgcgtacgtt ggttttctgc gacgtgccgg cgttttccca cgaggcgttt gcgagacgga
     1021 caaccgggga acgatgagcg ctgaagtgat cgcgtttcca gcgtttgttg cgggggaagg
     1081 gccctccctg gggggctggc ctccggcagg gcgtagcacg ttaagaaggt atgctgaatc
     1141 ccacctgaag gttcccgaag gcagccgcat tgtgaatgct catggtcggc acgcgcgtcg
     1201 ggttctccca tctgtattcc cactatttcc atggacttcc tgtaaggcat acgctgcgaa
     1261 cgtttccgcg cagcgctggc gaaaggcata atccgcatgt tgcgaaaagc attgccaccg
     1321 aagtatgcgg cctggctttc tgagcagaat attccggaga gcgacatcct gctctgcacg
     1381 gacaatgata tcacattcga aggcgagtac gcggaacgct gggttgtcgt aacgcgcgac
     1441 gccgtgatca tgctccgaga gagggacggg caggttcagg cggcgcgcag cttcaaaatg
     1501 gctgacatcg agtccgcccg gatcgatccg cgtgtcggca gcgggtttct gcaggtcaag
     1561 gttcacggcc actgggcgga ggtggcgcgg ttctccaacg cgcgggccat ctggttcgag
     1621 cgcattgcgg cgaagatcac ccgtttcaag gagacgggcg agatcatcct gaccgaagag
     1681 gacgagaaag aacagaaccg ttgtcccacg tgtgggctcc tgcttcggag ctctacggac
     1741 atctgccccc ggtgcatcaa caaaaatcgt gtgttcgggc gattgctgcg aatgatgcaa
     1801 ccgtactggc cgtgggcgct gggaacgttg cttcttgtgt tcacgggtat cgggctggac
     1861 cttgttccgc cgcagttgtc gcgcatcctg atggacgaca ttttcgtgca cgacccggcg
     1921 cgcccgtatt tgatgccgga gtgggcgctg cggctcatga atattacgcc gggaacacag
     1981 atgaccccgt cggagaaggt gactctgctt ttcttcgtcg tgttcctggg catggcgggg
     2041 gttcagcttc tgagaatcgg actgaatatt gtgatcggtc ggctttccgt caccgtcgga
     2101 acaaaaatta cctacgattt gcggcataag ctgttttcga ggctttccga gctttcagtc
     2161 cggtattacg accggcacca ggtcggcatc ctcatgcagc gggtgacggg tgacacggaa
     2221 agcctgcatg gattcgtgtc ccagtcgacg cagggcttcc tggtaaacat actgcttccg
     2281 gtgttcatcg gcgctgccct gttcaaaatg aatcccacgc tgacgttgta cgtgtttatc
     2341 cctgcgccgt ttgtgatcat ttcgacctac tatttctggg agttcgtgta cccgaactac
     2401 tacaagtact gggacagcca ttccaaaatg aacgcgatgc tgaactcggt gctttccggt
     2461 gtccgggtgg tgaaggcatt tgcacaggaa gagcgtgaga taagccgttt caaccatttg
     2521 agcggcaatg tgcaggcatc gcgccagcgg gtggacaagg cgacgggaac cttctacccc
     2581 atcgtcggct tcattttcgg tctgggcggt ttgatcgtct ggtacgtcgg tgggaggtat
     2641 gtgcttgaag ggaaggggat gacccttggc acgctgacag cgttcacctc ctaccttggc
     2701 atgttctacg gcccgttatc cagtctgacg tacatctctt catggctcac cggcttcatg
     2761 acgtccgcgc agagggtttt cgaggttctc gacacggaac cggagcttca ggacactgat
     2821 tcggcgctgc atctgcacat ctgccgcggg gaaatcgaat tcaagaacgt cacattcggg
     2881 tacgataggt acaatcccgt gatccgcgat gtcacggcgc acatcaaacc gggtgagatg
     2941 atcggcatcg taggccgctc cggatcaggg aagacgacgt ttgtgaacct tctctgccgg
     3001 ttctatgagg ttgatcaagg tgaaatatcg attgacggga tcagcctccg ggagcttcac
     3061 aaggacgaca tccgtcgcaa catcgggctc gttctccagg aaccgtttct tttccgcgga
     3121 accatccgcg aaaatatcgc ctacgggcga cctgacgcgg acatcgagaa cgtgatgaaa
     3181 gcggccaagg cggcgaacgc ccatgatttt atcatgcgcc ttgccgacgg ttatgacacg
     3241 aaactcggag agtacgggtc gggcctttct ggaggcgaac ggcaacgtat cagtattgcg
     3301 cgcgcactgc tttacgaccc ggcaattctg attctggacg aggcaacctc ttcggttgac
     3361 actgagtcgg agaaacagat tcaggaagcc ctcgccgttg ccacaaaggg gcggacgacg
     3421 attgcaattg cgcaccgcct ttcaaccctg cgcaattcca atcgcatcat cgtgatggac
     3481 gatggtcgca tcattgagat gggaacgcac gacgaactca tcaaaaagga aggcacgtat
     3541 taccgtctgg tcaaaatcca gacccaactg acctctgagc cgaccgtgga ccgccttgag
     3601 cttgagaaga aggaacagga ggcagcagtt tccgacgaaa gttccgaaaa ggaaacggaa
     3661 gaagaaacgg aaaaggactc cgaggaaaag actgacgccg aatccactga gccatctgaa
     3721 gacgaatcgt ccaaagattc cgagcaaacg tcgtgaacgt ttcgcacgtg gagtaacacc
     3781 atggaagaat cgactacaca gaaacccacc gaatcaggat acaaagggct ggtgttcctg
     3841 gatcccaagg acatcaccat ccatgagtct gagcttgccg gaatgaacgt gacggtaggc
     3901 ggggaaacat tcgaggaagt ccgcgcctcc ctcgcattac cgattaccga acccgaccgg
     3961 tacgtaagcc tgcggatcgg cgccagcaag ggggaggagc aggaaatcgg aatgatccgc
     4021 gatgtgaacg aattgtctcc cgagaatcag gtgctggtac gacgtgaact gcggaaacgg
     4081 tattttcttc atgtcatcta caaaatcctc tcgctgaaag agaagttcgg cttcctttac
     4141 tttgaagcag agacaagcaa ggggctgagg aaattcgccg ttcgctggga atacaacagg
     4201 gttcaggagt attccgaatt cggccgcatc atcatcgaca cggacgacaa ccgatacatc
     4261 atccccgatc tgcgcaaatt gagccctgag gaatacaaga acttcacgcg gtacatctac
     4321 tggtaagacg acggaacgga aaagagaaaa aaggaaacag acgccgcacc acggcgtctg
     4381 tttccttttg tgaccggttg cgcagccttc acccgcctca cttgcccgca gcaatccttg
     4441 cggtgagcgt cttcttggtg tcctcgtaga gccgcaccat gcgttcccgc gacgcatcgt
     4501 cgtacggcgt ggaaagcgtg cgcaggtact caattcctcc gttgacaagc tggagcatgt
     4561 gttcggcggc ggggccgtca aaaagaacgg aaccgcgggc ggcgatatat actggagacg
     4621 tgtgggccac cagatatccc ggctgatttg cgtgccccac gcagcgggca gcaatccatc
     4681 cgcttcccga gaggacaacg ttggccttgc agcgaagcgt tttcgctccg gcggcggatt
     4741 cctccgacgc caccacacgg ccgttgcaca ccacttcgat tttcccgagg ctccacgctg
     4801 attccgcgat ggcatccacc tcgtaggtgc ccgcacgggt cctcacattg atcacgtctc
     4861 ccacgccatg cccgtccacc gaaaggtcaa tgagcggccc ggtagtggag attgtgcggc
     4921 cggcccggac ggcgcgtgcc catgcgtcgt aggtgaaggg ttcatttgta tcaacccgcg
     4981 cgtaggtgcg gatccagccg aggggacacg acgcgctcat cttgtccgtg ccaccggcaa
     5041 ccgcgacacg atacccgcag ttcaggtaac ggtaccattc ctgcaacggg aaatttccgc
     5101 cctggaggga gccggtctcc agtgcgtcga cgagaccggc gatgatgggt acgggatcct
     5161 ccgtacaccc gcagtagggg aaatgcggcc tgatgactac gccgcctttc tggcggtttt
     5221 ccatcgccca ttcggcgagc gtccggtagt ccgggtcacc gatccaggct tccgttggtc
     5281 ccccggcgca catgggaaca accggcattc cctgcgttcc cagcatggac atgtggccga
     5341 gcatgtggtt ccggttttcg gtgcccacgt acacaatggt atcgttttcc gcgacaccca
     5401 cccgaccgat gtagtctccg acgttggtga aaagccgccc ccactgagat gccagcaggt
     5461 tcaccacgtt gacgccctca gcctgcgctt ccagccacgc ggtatgcggg ctcaggaaat
     5521 ggacgtgcgt atcggcggta acccatccgg cgcttctgag gtccttccag cgattgacgc
     5581 ggagttccag ttccttttgt cccggttgaa ccgtaaccct ggcgcgtgtg ggctgatatt
     5641 caaacccctt gttgatctcc acgaacacgt cgcctacggg taattcggtc cggaattcgc
     5701 ccggaacgta tgcatagctc atgtcgcgaa cacggacatc cgcgccatag tcttcgaacc
     5761 agttcgtgtt cacctgggaa tggtgcccgt agggtggcaa atattctccg tgcggtccgg
     5821 aaaaatgaac ccggacaggt gtcggcctgc cggtgctcga gtcgatgatc cggacattca
     5881 gccattgtcg ctgccgcccg attacctcga gtttcgcttt cccggactcc gagacgcctt
     5941 tttcgaagac ctcgcccaag cggaacgagt acgctttttt cttcccttcg agccgcagag
     6001 acatcgtcgc gtcaggtgct cccgcggctt cgatgaggtc ttcggccccg gtgtcgggtt
     6061 cacgagcacc cgataccccg gcatggggcg ccccgagcca ttcttcgttt ctgggaccgg
     6121 tggtgcgttc gattcgcgtg atctcgccca ggtcgagctc tgcccgtgtg acgtgtcgtg
     6181 gcatgtcgtc gaacgtcacc cgataatgcc gtctcggcag gtatcgcagc gggtgctgct
     6241 cgccgaaata cgccgtaaga ccggcaacga cgaaaggagc atcgaccaat ccgcggatgg
     6301 tgagggaggc gggttttctt tccgggtgcg ggttggggag ggcgtacaga actatcgcgg
     6361 gctgttgccg aacatgttcc tttccgaacc cgaactgcgc aacaccccag cccatatcgg
     6421 tggtcggttt tgtcggatca atcgcggtcc acgcgtcgaa ctggacggcc aaccacggtg
     6481 ggccggggct tgacgccatc gggacctcga accgggcacg caccgtttgc acatacgcgc
     6541 tctcgtcatc gtacgtgatt acgtactcgg cgaccggcaa accctcctgc ggaggcaggg
     6601 ttttgtcatc cggcaccttt tcgaaccacg catgaagaag gcagagatac gaaatcgaag
     6661 agccattgtc cgccggcaat gagactgtca cgtcctgcgt atccctcgcc agcatgataa
     6721 cgcgtttgtt cttgcccttg cccaaccgga acgggatgcc ccaggagacc tgtttccctt
     6781 ccgggaatgc cctgacccgg gtcgcaatat tgctccacca ttcgtcatcg ggaacctgct
     6841 tcggccagtt gaagagactt tcgagggaga caggtttgca gaggagcacc gcaccgagat
     6901 cacgtttctt tcccatggaa cacccccgtt cgtggccttc ccacatggtt caaaaggcgc
     6961 gatgatatga cggtagggta acgaacttgc cgtagggaca caaccacccc ttccgtgccc
     7021 ggtgccacca tgcgtccggg tttcgcgtta tccttcacct ctgtacattc tgatagcgtg
     7081 gttctcgcgg caacacgcgt tttctggaac tatctgatga ttcgcacgga cgtgagcatt
     7141 accgcggagg cccttctccc acaaatcgaa gctgcgtggg ggctttccgc ttcaaaaatc
     7201 gatctgctga ccagaaacag gtctgcaacc gacggatccc ctgtatttac ggtgcgtggg
     7261 cagtacacct ccaaaggctg gaccgagtgg acgcagggat ttgagttcgg ttccgcgctc
     7321 cttcagtttg acgcgaccga cgagcggcgt ttcctcgact ttggcaggag caaaacgcgc
     7381 gcggtcatgg caagccacat cacgcacatg ggagttcacg accacggttt caacaacatc
     7441 agcacgtatg ggaacctgcg tcgcctgatc ctggagggac gattcgcgtc ggacgcggcg
     7501 gaactcgaat tctgtgaact ggccctcaaa tgcagcggcg cagtgcaggc acggcggtgg
     7561 acacccactg cggagggcgg ggggttcatt tattcgttca acggtccgca ttcgcttttc
     7621 gccgacacga tgcgcagttt gcgctcgttg tccctcgcgc acaggctggg gcacgctctt
     7681 ctggaagaac aggacgcgcg ggtttcgctg ttatcgagaa tggttgccca cgcggaaacg
     7741 acggcccgtt atgctgtttt ctacggcgag ggccgtgacg catatgacgt gcgcgggcga
     7801 gtggcacatg agagcatatt caacatcaac aacggggtgt accgctgccc gaacagccag
     7861 caaggctatt cagcgttcag cacgtggacg agagggctgg catgggtgat gctcggcttc
     7921 gtggaggaac tggaattcct ggaaacggtt ccagacggcg agctggagcc tttgggaggc
     7981 cggacgcgtg tcgaggcgat gatgcggaga gcggcagagg ctacgtgcga cttctactgc
     8041 cagaacagcc ccaccgatgg gattccgtac tgggatacgg gcgcgccggg tcttgccgcg
     8101 ctggggtatt acctcgaccg tccggcggac ccgttcaacg actatgagcc ggtggacagc
     8161 tctgccgccg caatcgccgt tcaggggctg ctccgactag ggaaatggct ttctgatcac
     8221 ggaaacagcg agacgggggc gcgttactgg cagacgggcc tcaccgtgct ccacaacctt
     8281 cttgcggaac cctacctttc gaccgacgag aaacatcagg ggttgctcct ccattcggtg
     8341 tatcaccggc caaacggctg ggactacgta cctgaagggc gtcgcgttcc ctgcggggag
     8401 tcgagcatgt ggggtgatta ccatctgcga gaagctctgc tgttcgttca gcgcgttgcg
     8461 ttgagacagc catacctgac gttctacacc ccgtgacggg aactcctgcc gtgaccgaga
     8521 tacgccctat caagggaatc aagacagtgg aggacttccg cgcgtacgtc gaagcgcgcg
     8581 ggctggacct gcactgcgag gattcgattg ccccggcggc ttcatcgccg ctttgcgagc
     8641 ctctccatgt gtacggaaga accatcggaa accgctgggc agtgcacccg atggaagggt
     8701 gggatgggac aactgatggt ggtgcaacgg agctcgttat ccggcgatgg cgtcgcttcg
     8761 gcgaaagcgg cgccaaactg atctggggtt gcgaggcgat ggcgattcgt ccggatgccc
     8821 gcgcaaaccc caaccaattg atcatctcgt cccaaacgga gtctgatctc gcgcggctgc
     8881 ggggtgttct gcttcacgcg caccgggagc ggttcggaac caccgacgac atgatgatcg
     8941 gcttccagtt gacacattca gggcgtttct gcaaaccatg ggacaaaggg aagttcgagt
     9001 cccgcgtggc gtaccggcac ccgattctcg acaggaaatt cggggtgacc agcgacgcgc
     9061 agatcttccg ggatgacgaa atcggcccgc tcgtggagga ctacatcaag gcagcgaaga
     9121 tcgcgcgaaa cgtaggcgct gatttcgttg acatcaaaca ctgccacgga tacctgctgc
     9181 acgaatttct gggcgcgaag acgcgcaacg gaccgtacgg cggttcgttt gagaacagaa
     9241 cccgcctcct gcgggaaatt gcggctggaa tccgggcgga agttcccggt atgctcctcg
     9301 gagtacggct gagcgcgttc gatacggttc cctataagcc agatccggag aagagcgcag
     9361 gcggtgtcct gggacccggg attccggaag attattctgg gtgcctgccc tacgtgtacg
     9421 gctttggcgt caacgaaacg gaccctgttc aatacgatct tacggagacg tgtctgttcc
     9481 ttgagcttct ccgcgaactg gacatccggc tcgtcaacat tacaggtgga agtccgtact
     9541 acaatccaca tatccaacgc ccggcgattc ggcctcccag cgacgggtac gctccgccgg
     9601 aagacccgct tgtcggcgtc gcacggcaca taaacgtcgt caaatacctg aaagcgaggt
     9661 tccccgatct ggttcttgtg ggaaccggat atacctactt gcaggagtac ctccctcacg
     9721 tggcgcagta ctatctgcgg catcagcacg tggactcagt tggaatcggg cggctggtat
     9781 tgacgtaccc gcatttcttc gttgacgcga caacgaaggg cgtgcttgat gcgaggatca
     9841 tctgccgcac actttcggac tgcaccacgg gtccgaggaa gggccttccg agtggctgtt
     9901 accctttgga cgagttctac aaaacaagtg aatatggcgc ccggttgcgg gagctcaaga
     9961 aagcacgttg atcttcgggg gtgagatgga ttcctcacgt gaactcacga ttcaggacgc
    10021 tctcaatgaa gcgttgcgga cggagatgcg tcgcgacgaa cgagttttct gcatgggtga
    10081 ggacatcgcg atctacggcg gcgcgtacgg ggttaccaag ggtcttctgg atgagttcgg
    10141 cgcggaacgc gttcgtgaca ccgcgatcag cgaagcggca atcatcggag ccggcgttgg
    10201 cgctgcgatg accggcatgc gacctgtcgt ggaactgatg tatgtggact tcgccggtat
    10261 cgccatggac cagatataca accaggcggc gaaaatccgg tacatgttcg gcggaaaggc
    10321 gaaggtgccg cttgtaatcc gtacgcaggg gggcaccgga aaacagatcg ccgctcagca
    10381 ctccaacagc ctcgaggcgt ggtttctgca catacccggc ctgaagactg tgatgccgtc
    10441 cacaccgtac gatgcgaaag ggctgctgat ttccgcgatc cgggatgata atccagtcat
    10501 gttcatcgag cacaaggcaa tttacaacga gaagggaccc tgtccggaag gcgattacac
    10561 ggtgccaata ggaaaagccg ccattcgccg ccccggaacg gacgcaacga tcgtgagtta
    10621 ctcgcggatg gcgctgagga gcatgtccgc agcggagacg ctcgccggag aaggcatctc
    10681 ctgcgaagtg attgatttgc ggaccataag cccgtgggat acccacgcag tggttgaatc
    10741 cgtggccaaa acgcaccgcg tcgtgattgt ggaagagggt acgcgcacgg gaggggtggg
    10801 tgcggaaatc tccgcgagaa tcatggagct cgcattcgac gagcttgatg cgccggtgga
    10861 gcgcgttgcc ggcgtggata cggtcattcc ggcaagcgcc acgcttgaag cggcagccat
    10921 cccgaacgtg gaatccatca ttgccgccgt tcggcgaact ctccaataaa aagaaggcgc
    10981 tcaagaacac ctcctgagcg ccattctctg ccgatgaagc cctcggatca ctcgctctgt
    11041 tcgatggttc cttcgggctc ctttgtttcc gtgtcttcta cggtttcggg ggctttctga
    11101 ccggctgcgt cgacgacttc gcctttcatc gtcgccgtaa ccccgtgggg caacttgacg
    11161 aagaactcga atgctcccag cgcgcgaacg ggttcttcta tgacgatgtg acggtggtct
    11221 acgtcaaaac cgtgcgtttt cagtgcttcc gcaaggtcgc gattggtaac ggagccgaaa
    11281 aggcgccctt cctcacctgc ctgcatagga atgaccaggt gcaccggttc gagcgctgct
    11341 ttgactcgtt cggcttcctg ggtctctttc gaaacgcgca cgaggaactt gcgcttttcg
    11401 tcttcccaca cacgcagatt ccgttcggtg gctggatacg ccaggctgcg gggtatgagg
    11461 taattgcgcg catacccgtc ttttaccgaa acgacgtccc ccgctcgccc gagtgtttcc
    11521 acgttatctc tcaaaatcac tttcatcgtt ttacctcgta tcggggcctg tctacttgat
    11581 catttcggat acgtacggaa gcagcgcaat ctggcgcgcg cgtttcaccg cagtggtcag
    11641 catgcgctga tggcgttcac acacgccgga cattctgcgc ggaataatct tgccgcgatc
    11701 ggaaagaaat ctgcgcagca gcttctcatc tttgtagtca atgtgagtga tcttcgcttc
    11761 gcagaacttg cacaccttca cccggatctt tttttcccgt ggcatctgtt cagccctcct
    11821 cttcttcgtc aagggcgtca acgtcaacac ccgtgtcctt cacgcgttcg cggtctcccc
    11881 ggccccgttc ctcgtcatcg tcacgcgagc tgtcgactgc ttcgatggcg ttgaggtctg
    11941 cgggtgtagc aggcggcatg cgatcgtagc ggacggtcat cgtccgcagc atgtcttcgt
    12001 caaggagaag ctgcttctcg aggatgttga cagcggttcc cggggcttca tagaagagga
    12061 acgtgtaatc ggcctgcgtc ctccactggc catctttgcc tttgatgtca tacgccagtt
    12121 tccggacgcc acggcggtca acctgcaagt ccaccgcccc ggcttttgtt accaggtcta
    12181 cgtatttctg cactttctcg gtgatagcag tctcctccag taaggagtct atcaccaggg
    12241 ttgtttcgta caacggcata cgcgcaatcc tcccgatgga tcgacgttga gtcaagcctc
    12301 cgccgtcgcg ggcggaagcc gagaggggaa acgccggcga gcgattccca gaaaagactt
    12361 ataaagatag ttatctcaat cgttctttgc caattgtcga acagcccagg gcaggtggaa
    12421 gctcccggtg ccgtcaccca aaaaacgcga agggcggggt tcgcaccccg ccctctgcat
    12481 tccctgcacg cgcgctcact agatgaacaa tggcgcgaga accagagtaa ttgtcgaaag
    12541 gagcttgatc agcacgtgca acgaaggccc tgcggtgtcc ttgaagggat cgccgacagt
    12601 gtcacccacc acggcggctt tgtgcggatc agaacccttg ccgccgaggt aaccggcttc
    12661 gatgtgcttt ttcgcgttat cccatgcacc gccgccattg ttcaggaaca acgccatgag
    12721 gattccggct atcgtgccaa ccatgagcag cgccgcaaca gtttcggcgc caaccggcga
    12781 cttcaccact gcactggcac ttttcacacc ggtaacgaca acggatgccg gctggatgga
    12841 aagcgccgaa tccgcattgt acgaacggac gacctcgacg ctggtggcgc gtatgcggct
    12901 ttgcccgatt gcgataaggc tatcctcgtc cacgtacttc tttttggtga tcgcccatac
    12961 ctcgtcgacg agaggcctcg agaggtatgc cgtgtctgcg gcaaacgcga aggtgttcgc
    13021 tgcgccgtac gtcatcacgg attccgcggc acacgcgacg gccggggcca cttcctccgc
    13081 gggcgcttcc gtcatcattt cgccttccgc gggagcagca gtattctgtt ccgcgactgc
    13141 tgcttccggc agagcctctg cacccgcaac ctgctcaact gccgggatat cagccttgaa
    13201 gctgacgata cccagcgggc gccacgcgtc tccaccgaac accatgaagc ggaacaccac
    13261 acccactacg atcggcataa gcacggccag gagacccggg agaaccatct cacggagtgc
    13321 acccttcgta acgatatcaa cgcaccgacc gtaatcgggt ttttccgttc ctgcaagaat
    13381 gcccggcttc tccttgaact ggtcacgcac gtcctgaatg acatactggg cggcgtggcc
    13441 gactgcacgg atcgcgaacg cggagaagag gaacacaagc atcgcaccga ggagagcgcc
    13501 gacgaagact tccggtttgg cgatatccac ggtggaaaga atcatgccct tgccctggag
    13561 gtagatgttg acctcgtcga tgtaagcgct gaaaagcagg aaggcagcca gtgccgcaga
    13621 accaatcgcg tatccttttg tcaaggcctt ggtcgtgttg ccaacggcgt caaggcgatc
    13681 ggtcttttca cgcacctctt ccggttgctg gctcatctcg acgatgccgc cggcgttgtc
    13741 ggtaatgggt ccgaaggtat ccatcgcgag gatatacgcc gcggtagcca gcatacccat
    13801 cgttgcaaca gcagttccga agaggccggc atgcgcaaag cccgaactcg caccgagata
    13861 gtaggacgcg agaagagcga tggaaatcac gatgacgggc atcgcggtgc actcgagacc
    13921 gacggcgatg ccagagatga tgttcgtggc gggcccggtt ttgctcgcgt cggcgatcga
    13981 cttcaccggg cgatacttcg attccgtgta atattgcgtg atgtagacga atgcgattga
    14041 ggtaatgacg ccaatgatgc cgcacaggaa gaagtgccac cacgcgcttg gtgccgacgg
    14101 atggttcagc agccagtaac ttgcgatgcc gaagccgatc atcgccagaa cgacggccca
    14161 gtagtagcca cggttgagcg acgacatcgg gtcttcgtgc tctttcactt tcaccatcat
    14221 gattccgacg atggaagcga tgataccgaa ggcgcgggca ataagcggga acatcatgac
    14281 gccgagaatc cagagcggac tggcgtccgg gacggccttt gccatgctgg ccgcaaggat
    14341 catcgcaccg atgttctccg ccgcggtaga ctcgaacaag tctgcaccac ggccggcgca
    14401 gtcaccgacg ttgtctccca cgaggtctgc gatgacggca gggttgcggg gatcatcttc
    14461 cggaatgcct gcctcaacct tacccacgag gtcggcgccg acgtctgcgg ccttcgtata
    14521 aatcccgccg ccgagctgag caaagagggc gacaaaggac gcaccaaatc cgaatccgac
    14581 gatcaggagc ggaatcttgg tgggatcaac tgcggtagtc agtttgaccg cggtgtacaa
    14641 cccggcgatg cccagaaggc tcatcgaaac gaccatcagg ccggagaccg ctccaccgcg
    14701 gagtgcgatc tgaactgctt cgttgagatt cttcatcgcg cccgtcgccg tgcggatgtt
    14761 ggcgcgaatg ctgacccaca ttccgatgta cccggcgaaa agcgaacaca gagctccgag
    14821 cacgaacgaa agagtgatcc agaacgccag accggtggcg gttgtcacgg gatccgtcgg
    14881 catatggttg cgtaggaatc cgtaaccgat gaaaagcaac accgcgaagg ccgcagcgag
    14941 ccagataatc gttttgaact gccttcgcag gaaggcctcg gcgccttcct tgatggcgtt
    15001 cgagatatcc tgcatcgcct ggcttcccac gtcttttttg agaacccatt tggcaagcgc
    15061 ccaggcgacg atcagcccga acacgctgat gccaaggacg atactcagca acatgctttc
    15121 gctcattcga agagctcctt tcccaggaat ggagttttcc accaacccca acgggaggcg
    15181 cgcccaacgg cctccctctc atttcgtcat cgtttgcgag ggcctcgtct tcccttcatc
    15241 cgatgacgca gaactctatg ctcctcttac ttcacttcac tccgtttttg ccggatcgcc
    15301 tgatgcttcc tcgttcattc cggccgactg cttatccgtt ttttcccaac ccgccaccgg
    15361 ttccgaccgg gaaccggctt tccatcggct gtacagttca gcggcgcctt caaaaccgcc
    15421 cgaacagaag gcgcggacaa ttccacccgc ctcgattgcg gcctgccgga cgacgggttc
    15481 ctcttcctct gaaaacggcg agaggacata atcaaccaac ccgccaggtt ccgatggacg
    15541 gccaatgccg attcgtaacc gcggcacatc ctgcgtctgg agggcttccc ctatcgacgc
    15601 cattccgttg tgtccgcccg cggaaccact tctcctgagg cgcagatcac cgatcggcag
    15661 gttgacgtcg tcaagaagca cgaggagatc cgccggacca accgtgaact gacgacacac
    15721 cgcaaccgcc gcttctccgc tcagattcat gaaagtcagc ggcttcacga ggacgcattc
    15781 ggaaccaact gccgggtaac cggcaaaaag aaacggtccg tctccacgta cccaccgatc
    15841 caaaggaatc aaggaatcca ggacgaacca acccacgttg tggcgagtcc gtgcgtaaga
    15901 aggcccgggg ttccccaaac cgatcacagc tttcacgctg tctacgactc ttcctcttcc
    15961 tcggtcgcct tcttccgctt caccagttcc ggctccgccg cagtcggctc ctcttctgca
    16021 ggcgcttcct cttccactcg aggcgcggaa atggttacga tggtctggtc tgtatccgtg
    16081 aggattcgca atccctcgag tttcagttct ccgaccgtta tgtgatcgcc tatttcgagt
    16141 tgtgtcacat caaccggtat gcgctcggga atggccaggg cctggcactc gatctcgagc
    16201 gcgtgcagaa cgtggtcgag aatacctccg gcaagcacgc ctttggaact ccccacgatt
    16261 tccacaggca cttcgacccg caccattgac gtgctcgaca cgcgcatgag gtccacatgg
    16321 agcaacgcat cattcaacgg gtgaaacacc ggctcgcgga agagggcctg acaggtttcc
    16381 ccgccttcga cagtcacgtc gatgattgcg gtggtgcggg cctggtgaaa tactctcgtc
    16441 agcgtccggg catcggcctg cacggaaacc gggttcgcac cggccccgta aagcgtcgcc
    16501 ggaacaagtc ctttgcgacg agcctgcttg gcatactgct ttccgtgccg ggtgcgcgcc
    16561 tgaaccgtga ggctgaactt gttcatgctg ggttgtcctt acgtgagaaa gtcgtctgtt
    16621 ttggtcgatg aataatccca gatggtggtt ggggcgggag gactcgaacc tccaagtgca
    16681 ggatccaaaa tcctgtgtct tgccatttga cgacgcccca gagaccgcgt cttcatgggg
    16741 attcacgggc aatgtaaccc tgttcttggg gaatagtaaa ttttaacgtt atttggcctc
    16801 tctgtcaagc ctgacacggc caccccgcag ttacatcccg tcgcgtgatc cgattgcgcc
    16861 gatgctcggt tcccgctagt ttcttacggg taccggacac gttctgacgt atctccatcg
    16921 ggaaagacca atgatcgcga ttcttgatta cgaaggcgga aacctcgcaa gtgttctccg
    16981 tgcgctggac cacaccgggt ttgatgcctg catcacgtgg gatttcgaga cgatccgccg
    17041 cgccgaccac gtgatcctgc ccggagtggg agcggcgaag gcgacaatgc accacctccg
    17101 cacccgaggt ctggacaaac tcatacggga agaggtgata gggggcggga tccccttcct
    17161 cggtatctgc atcggagttc aggtcatctt cggctcgagt gaagaggagg cggcggaatg
    17221 ccttggcatc ctgcccggtt cggtgcggcg gttcccttcg gtttgcgggg gcgaacaaat
    17281 caaagttccg caaatcgggt ggaaccaggt gcgtttcagc aaaccacatc cgatttttgc
    17341 cggcgtggag gacggaacgt cgttctactt tgtgaactcg tactacccgg tgccagcgtc
    17401 tgaagagctt gtctacgcaa aaacgacgta cggcgtcgag ttcgcgtcgg tagttggcaa
    17461 ggggaatctc gtcgcgacgc aattccatct cgagaaaagc ggtgtgcccg gtttgcgcat
    17521 gctggaaaac ttctgccggt gggatggctc gctgagctaa ccagcgcgta ggaacggaaa
    17581 ggatgctcca tggaaaagaa gacctgtctc atgctgggcg ccggtgggat ggccggttgg
    17641 tggattcgta acgcgttccc gcgatttgtg gatcgaatga cggtcgtcgg gctggttgat
    17701 atccgtgaaa atgtactgca tgaatcgggg aacctcctcg gaatccctga aagtgcccgt
    17761 ttcacggaca tgaaggaggc attcgcgaag acggaggcgg atttctgcgt aatcgttacg
    17821 ccgccgtggg tgcacgagca ggcggtgatg ctggcagtag gaaagcggat ggcgatattg
    17881 agcgagaagc cgattgccga ttcgtggaag gcttgcacgg ccatctaccg cgcggttgcg
    17941 gcggcggggc tcaagatgga agtgatccag aactatcggt atgaacccac catgttgacg
    18001 gttcgacaca tccttcgcag tggacggctg ggacgtgtca attacatcgt cggccggttc
    18061 gcagatgatt accggaggta cggttcctgg gggcaattcc ggcacgaaat cccccacagc
    18121 cttctcgtgg agggcggagt gcaccatctg gacatgctgc ggaatctttc ggggggcgac
    18181 acggcgctga taagtggctc ggaatggaat ccgccctggt caagtttcaa aggggagtcc
    18241 aacgcgctgt ttactctcac aatgaccaac tctgttcgcg cccagtacga agggagctgc
    18301 aacgaggcgg gcgtggtgaa cgggtggcac agggagtatt accgcgtgga atgcgagtat
    18361 ggctccgtga cggtggacag tgacctcgtg gtgcgaatct gggagcgttc cgagggaagg
    18421 tcctgcaaga tgacggaagt gcctttgctg aaccccacgt atgccggcca cctctggcaa
    18481 ctggacgagt ttctatcctg gctggacgga ggcagagaac ccgacactgt gctttcagac
    18541 aacatgcaga gcgcagcaac aatgtttgcg gcaatcgtcg cttcggatga cgctgcaacg
    18601 gttgacgttc agaagatggt tcgggcggcg atgcaggcgt gaggttaacg aaacgaagga
    18661 accagtagcc ggctcttttt gtcggtgggg cggctgtttc ctccttgacg aattatcact
    18721 gcgatttctg attggcaacg tttgtgaaaa ccatcccgct tcttgacagc agaatcgtcg
    18781 cacaggcgtt cgcattgcgc cgtgttgtcc aacacccggt gctcgtctca gaggatgttg
    18841 ttccgggaga cacgcaggcg tacggcagcg ttctgaggga tcgcaaaggc gtatggcgga
    18901 tgtggtacct cggaccgccg atctattgcg agtattacgc ggaaagcgag gacggtctga
    18961 actgggtgcg gccacacctc gatttcgtgg caccggaggt gagaagcgag gtcgaggggc
    19021 ccaacgcgtt cctttgccgc aaccagaagg acgtcaaggg cgcgtggctt gtcggctcgt
    19081 acggcccgga agggttctgc gttctggatg ccgagaagac acctcatccc gcggcgaaag
    19141 cgaggttcac ggcgctttac ctcgcgagga ccgagacgga aagcgggatg tacatcgcct
    19201 actcggacga cggcacgcgc tgggtagcag actccggcat gccggtgatc cccggttggc
    19261 gggatacttc aagcgtcttc ttttttgaca agaggaagca aaaatacgtc tggtacgggc
    19321 ggccagaggc ttatgcggcc ccctccatgc acgcgaaccg cctgctggcg taccgggaaa
    19381 gcgacgacct gacacactgg actccggaca gaacagttct cgacacagat gatgcggatg
    19441 ccgatcctca ccacctcctc gatgaaagag cgttgcacgg aggacaacag acgatcgcca
    19501 ccgcggaaca acgtgcccgg gtgtgggcgg aactcacgga ggggcagacg cctgacaccg
    19561 atcggcctct ggttcggggg cgcaaccggc aatggtatgg aatcaccgcg ttcccgtacg
    19621 ccgacctgta cgtggggatt gtctggatgt acgatttgcc gaccggcagc atgtggacgg
    19681 aacttctgca cagctacgat ggcgtggact ggcgcaggga agcgattcgc gtgccattcc
    19741 tcgaagcgat tccgggaacg tgcatgtgca cgatggcatc cccgcctgtg gaccttgccg
    19801 aggaaacgag gctgtactat tccgtgtcga accagagcca tcaccgggtc ccaaatccct
    19861 cggtgaagaa agggatccgc atcgcggcgc ttcgtcggga ccggtgggtc gggtacgtgg
    19921 cgggggatgt tcagggggaa cttctgacgc aggtgatgga aaaaccgcgc gacctgaccc
    19981 tcaacgcgca gactgaggga gatggctggg tgcgtgtgga agtggacgac gcggatgggc
    20041 gggctataga cgggttttcc gcggacgagt gcgttccatt ttctggcgac gatctgggct
    20101 ggcggcc
//
LOCUS       DAOSOO010000035        19891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2713_length_19891_cov_5.832224, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000035 DAOSOO010000000
VERSION     DAOSOO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19891
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_19891_cov_5.832224"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PLT86_04235"
     CDS             <1..1139
                     /locus_tag="PLT86_04235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT35858.1"
                     /translation="VLHLLGSLGAGGEERQTSTLVKGLCGSRWEPIVCGMRGGALAEE
                     FARHARTIILEKRRKVDLAALARIRAIMDEVRPAVLHCHLSTANTWGRIAALMRPEKR
                     PAVVVSEGGIETWKTVVHLAIDRALLGVTDAVVGNAEAIAHYLIDHDRIPARIVRVIP
                     NGIDLARVQAKLHATEEERVSRRKELGLTSDHFVVGHVGRSCAVKGLDTLAAVIEQMV
                     RLNPDFRLVRAGQPPLPDEIEPAEAFRRDVEARGVADHVIWHPYTPDISSVLMILDAL
                     VQTSLSEGLPNAVLEAMAMDVPVVATAAGGTSEIVRHRETGWLTEIGEARGLCEGLEH
                     VRNDPEESRLWACRARTMVEHDFSLARMVENFCDLYDRVAGLHP"
     gene            1284..2213
                     /gene="thrB"
                     /locus_tag="PLT86_04240"
     CDS             1284..2213
                     /gene="thrB"
                     /locus_tag="PLT86_04240"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT35859.1"
                     /translation="MGKRPQGWSIRVPATTANLGPGFDCFGLALQRYLNLFVTVRGEK
                     GAWHITLHGEGAGILPRDRTNLIYRALVLGSEVDAESLPGISIRMENAIPLARGQGSS
                     AAAIVAGMAAAQLLTVGKIDPDQLINRATLVEGHSDNVAAAVKGGLVLTRESPSGVAI
                     RPIPTRDGALFVSAVPEFELTTHTSRSVLPQQVPLGDAVFNLRAAAWMASAWAAGDWE
                     AVGNAMEDRLHQPYRAPLIPGFDDVCRAARAAGALGACLSGSGPAIVALALHSSDAVA
                     RAMEQAWARHGITSRAEVTHVDTDGLTVAPLEA"
     gene            2226..2459
                     /locus_tag="PLT86_04245"
     CDS             2226..2459
                     /locus_tag="PLT86_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35860.1"
                     /translation="MGRRKDYPLPSSVGDEKKWAKGEWWEWLAIFFSIAALWPVILRW
                     DGIIWDLALFGALVLMIVVFLRRARRMKRSWKQ"
     gene            2479..4593
                     /locus_tag="PLT86_04250"
     CDS             2479..4593
                     /locus_tag="PLT86_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35861.1"
                     /translation="MNGSNFPSAGWEITFREGWWLWSALGLALILLAVFSYRRTIPPL
                     PPRRRALLFGLRLLALCAFILAILNPRLERRGMEPRPRRIALLVDESRSIARAGQESA
                     VRSILASAQMKRVSALADVVALGFADTVRVLDEATLSFRGPATEIGEAILSQRRVFPP
                     PDAIILITDGSNTSGSEPVRAAQEAALPTYVVGVGNPHPEPDARIVGVAAPPYAVEGK
                     PVSVTVAWEHTIPGMRASTMRLFHQGREITRAIVLPPETGARADVDLTFTPSARGMQT
                     YTVELEGVPGESRTENNRRTFSLDVVKAKKRALIIAGTPSADVGFWNRFFGAREDYDP
                     AIWYATPFRRVAPLSPDSIAGTDLIVWLDPAGNTLPPVTQDAVARAVEEGCGLLCVPG
                     TNSVSPRLREILPVELTGTRFEPGQFEVRLAAPLFAHPISGMDPGFAEWSSWESVPPL
                     LGLVSGLRPRQGATVLVTGDAGPVAVAGHAKKGRVLVFGGAGYWRWDILPRGMGIENP
                     AGTAFWKAAIRWLISREASQMVRVQTGRPFYRLGEPVRVDIFVSDEASKPVDNASVIV
                     ATDGPDNWTGRATLSGPGRYTVWIRGLLTGAHTMRAEAFSGTTSLGTSETRVLVDASS
                     AEDDIVQMQTDVLSRIASAHPGSRFVTARDAEQLLASIPLSPVFEYSERTVSLGSAGW
                     VLCGIILLLTGEWGLRRTHGLL"
     gene            4560..6281
                     /locus_tag="PLT86_04255"
     CDS             4560..6281
                     /locus_tag="PLT86_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT35862.1"
                     /translation="MGFTTYARAALILATTCTTFSSPAEARRGGPESCPLVISEIMVN
                     PSGTETSREFIELFNVGTGSVNLGDYVVGDTNETDAIIAVSSGLVLKPGRFALILDPG
                     YFSANEPYPDIPAEALVVTIGDAAFGRGGLVNSRANTVVLRSSGGCEQRVSYLPEAVF
                     PGLSLEKIDVRGPDTPDNWAVSLVPDGTPGRPNSRRRNACDVSLRNASANPDTLHPPE
                     QLTVRFDVLNSGTDTTGPFPVILFADRDRDSVLSAAEDTLVRWNVRSLQPAETFAGVE
                     TIPVSRPFSGPLMLAAMLPGDENPRDNTQALRCVIGLPARSIVINEIMAYPSDGEGQW
                     VEVLNAGADPLSLAGSLLKTATGSGSADYGPLLYPGDYLVIAALPEGVRTRYGIGTPL
                     CGVTGGFPYLSRSASSSRRVSFLDATGTSIDEAHYPVPEQGRSLERADSASDGSNPAS
                     WAPSWAPARATPGLRNSASYPPPRGFDCMADPIPATDTVGIRIITPEHGSSVSLTVFD
                     MAGREIRTLLTRERLPAESHVRWDGTDGSGRQVPTGVYILHIEVVSPDEGKHFRRSQP
                     LVIVRRR"
     gene            6472..7725
                     /locus_tag="PLT86_04260"
     CDS             6472..7725
                     /locus_tag="PLT86_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35863.1"
                     /translation="MRMRSLLHVAVIAGFGIFVSPRGAVSQGGGGPQQGRIAGDPLPA
                     LATEWQQSFDAARQTNNSEALSLLSRAKTLLNQAETFRRRGETARAAANADEARLLIL
                     RANQILRTPPGRSPQIDATLRDAETLMRNAVAQGAPPDQTAQVKLLLDQARKAQTEGR
                     HDAALRLSATAQTQARQLWQRAVASRQLRQRCRVLESAAEPMVRRARTMADANGGDAV
                     KSAAERAQQFLQMAQSTDEATHPAAKARLLERAMQNAEAVLKTLDAATYSAHLASRSI
                     ADADAALARADEVASGDASGNDVNGELRRGRVVIANARQELASGNSIRARALAEEARQ
                     IAEQVLRSALGEVTEASVRAALQQTDALIERASAASGNETDPLLVEARNRQADARRML
                     ADGDLRRALAQTRIAARLAQRINAR"
     gene            7715..8164
                     /locus_tag="PLT86_04265"
     CDS             7715..8164
                     /locus_tag="PLT86_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35864.1"
                     /translation="MLADTIACGSPRPGFMASSAKRLLRAGWSLLVAIVCVSPACSPP
                     ARSPDVFPDSRSDESALEVASDLPASANLDEDFALWRETYELTRSASSDSICIAKADA
                     IASEAETQWLLGDREQAEVLWGEAFDLLSGTCADTILVEPVHPPDSR"
     gene            8363..9484
                     /locus_tag="PLT86_04270"
     CDS             8363..9484
                     /locus_tag="PLT86_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35865.1"
                     /translation="MESETTEVTTDAYGQLRVVSSGALGSWRNIILDNRFAAGMSSLR
                     YDFRGTGEWKNTSPVGVNLDGAVSARRFHGANSASRSSYIETSGEVAALSPLPHAYPG
                     TWGSVHSVRGVWYDRTSSVYTNAMSHWHGLALETTRDLSTWTTKAGVEHEFVPDSSEL
                     GYWGFSGSSFAMWVPSLDWLVSGGVIAAWKDYPAPGTHPDESSATLHARVTRRLNPSW
                     SLETGINGAVLAFSPADDVFPSTRNMELLAEAKWEPGLVSTRFGAGAGMQQSPSSLAD
                     EYSERFLQAGCNLISRTSGFSDVSIRAGKRTYRHPSAAYSSDYVFGEFTALVSGARVF
                     GVRAEAFVTFRSEHHEDDTENSRNLFITVDISRTMNTRR"
     gene            complement(9551..10468)
                     /locus_tag="PLT86_04275"
     CDS             complement(9551..10468)
                     /locus_tag="PLT86_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOT35866.1"
                     /translation="MTIRTRELVLISVAAGLSVALSFLRLFSMPQGGSVTLEAIPLLY
                     VAFRFGTGAAMAAGAVSGIVQLLLRPIIVHPVQVVLDYPLAMAACGVAGVFRTVNSNR
                     SVRVGLTVFLGVVVVFVAGLQWFELRRVSRTDEIEVQRSPDSRIVMWASADTILGGLQ
                     TKIVTYRNSAAGRSSVVGVTTAHGETARDWLDYGLSVVRAKQNEWLGNFLLLAAALAG
                     IALVAARLPLTSVALGVSLASFLKWGIHVISGAVFFSAYAPLGESAWIYSAAYNAAYA
                     IPQLLLALLLLPPLLRKGANNAGTADPGN"
     gene            complement(10449..11768)
                     /locus_tag="PLT86_04280"
     CDS             complement(10449..11768)
                     /locus_tag="PLT86_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT35867.1"
                     /translation="MDDSFSIWFLIGYAFLFGLSWVFGRAERFLSLRSVASVQALVDA
                     NRLQVPQFLLKSPAKRRAAISFGIGVLAADLILIAGVFVFLASVPGLSGAGLILAYAI
                     AALLGFVPLVVTHFVVPNLKTADDMSGPLLRFAAPLLVAWAVLTFPLSLFLDKILTHL
                     AHQPENREAREEALLALVESDEVDGVLEADKRDMISSIMSIDETLVREVMVPRVDMVA
                     IERSQSLNELLDLIRETQHSRIPVYDGKLDNIVGIVHAKDVLDAVSSSGGANGSTATA
                     EDAMRESHVLFVPMTKRVDDLLRELRKQKKHMAIVVDEYGGTAGLVTMEDVLEEIVGE
                     IQDEYDEEEQPYKWLDQKKLEVSAGMSIADLAQLLGAEFPEEQGYETVAGFLYHQFGA
                     VPSKGESTRYGPYTFTVTGVDAQRITTVDIEIHGDDARGTEFDDSDA"
     gene            complement(11811..12170)
                     /gene="ybeY"
                     /locus_tag="PLT86_04285"
     CDS             complement(11811..12170)
                     /gene="ybeY"
                     /locus_tag="PLT86_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT35868.1"
                     /translation="MAGEVWKRERGDVPASVAIVFVGPERMRELNRSFRGKDAVTDVL
                     TFDLADSADRVEGEIYVCPEYAAGQLRTYSTGLNQEIVRLVLHGVLHLAGHDHHTLAD
                     GKRMANHTRRYLRELAW"
     gene            complement(12354..14720)
                     /locus_tag="PLT86_04290"
     CDS             complement(12354..14720)
                     /locus_tag="PLT86_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT35869.1"
                     /translation="MRDHLQRLIRHDGVLVRLRRSLRGRLTEEYRKYLRILVGLVGIG
                     LFMALFPRERMNEYSSWREGMVAPREVIAPFSFNVRKNPLELETERRKARNSVPPVMR
                     MDPLVGLRNRRRVERFLDSLAGNPSPAAGTVSVSGIRPNRNAIQWLKASGGRNLGIAR
                     TVLVPVLERVYERGVVPVEDWQFLKEIVQRKVEAGAARGDSSDVVTVIRGDTLEYEQP
                     IGLMMNAEEARDELGQLITRVAMENVIQLSSDGLRALYAVAEAALEPNLAYDRTETAD
                     RQEQVMAAVPIYKRTVFKDERFIESHAVLTAEDIDEVGSLVSAQRARLRQAYGWQPFL
                     EVAGQLGIVLLVMVGVAVYLRTFHPPIWTRPAWLFLCILLAWIPLLAASFVAQNPFLP
                     VYIVPLALTAMLGTLLFGVEVGLVLVVGASLLGGAMLGFEYRVVIVNVVAGFVAAFSV
                     RNVRNRNQFLSAMLWLPLAIAFTTVAIDLIQDTTAKAMWKDVWPGAVNGFTVPILAMG
                     LLAVCEKVFSITTNMTLLELSDLNAPLLRELSLRAPGSYTHSIIIANLTESAAEAIGA
                     NALLARVGAYYHDIGKMLRPDHFVENQLTVRNPHDRLTPHMSALVVSAHIKDGIDLAE
                     RVGLPRQIIDFIPQHHGTMLMTFFYRKAQRMYGEENVSEEAFRYHGPKPQTREAAILM
                     MADAVEAAVRSLRERTPAHVQNTVHQIIRERLDDGQFDECALTLRDLDLIEKSFLPVL
                     AGALHKRIEYPEPITPEPLVKESQEEAATGHTHGESSEHHGNSGNRGI"
     gene            complement(14733..15683)
                     /locus_tag="PLT86_04295"
     CDS             complement(14733..15683)
                     /locus_tag="PLT86_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT35870.1"
                     /translation="MQHLFGPLDRNRKELEEEFHVSLVSRQGQLLVRGTEAEVAAAVK
                     ELDQRLRGDFVAPGPGTASPVALSKKRSGEGHPPTGSPAGPVILHTTNKVPISPRSRG
                     QREYCEAIARHDIVFGIGPAGTGKTYLAVAMAVAALKKREVTRVILARPAVEAGENLG
                     FLPGDLKEKVDPYLRPLYDALHEMIPPDKLKNYLETHVIEIVPLAFMRGRTLNSAFVI
                     LDEAQNTTTRQMKMFLTRLGADSKAVITGDITQIDLPAAVPSGLIDAKQILSGITGIS
                     FVSLSERDVVRHRLVQLIIRAYDRVESSHGASNNANQQGV"
     gene            complement(15853..16713)
                     /locus_tag="PLT86_04300"
     CDS             complement(15853..16713)
                     /locus_tag="PLT86_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT35871.1"
                     /translation="MTRTLYAVALGALLGLSAMAAAQTAPQMTMQQYEARLRECQNRQ
                     RAADSARAVVEQQIAQKRAELSSLDEQIAATNRQVLEAVAADSAAIRAYLGQLDGIIQ
                     QLQAMRQLPANQIVDARERGDLDRIAEQLAQLKTNRIAALPEAKAKIQTADQMIAELR
                     AVQRPVPEVRRDQYTVVRGDYLWKIAKKPEIYGDPYTWVRLYTANKDRIRNPDLIYPN
                     WVLGVPRNQAPGTYWVKRGDRMRTIAQEVYGDPSQWTKIYRANRDVIEAVSGGRRVIY
                     PNMILTIPQN"
     gene            complement(16790..17386)
                     /locus_tag="PLT86_04305"
     CDS             complement(16790..17386)
                     /locus_tag="PLT86_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35872.1"
                     /translation="MIVRRAAVCVLACGTALFAGCAPSAQPGARPAAGALAERACLEA
                     AAAETSAARKMAAYRASQTDFSAQQARLVELQRIRSRIATGRMSDEELATWEVEPAPM
                     QAAPAETTQPVVPAVTAPGDSASVEQAAPTAESQESVTPAVESEQGVAPAAEPQENVA
                     PAAEEQAPPTSESETTPAPDAETAPADSTAPGGESSAE"
     gene            complement(17398..17661)
                     /locus_tag="PLT86_04310"
     CDS             complement(17398..17661)
                     /locus_tag="PLT86_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35873.1"
                     /translation="MRRAPWGLVLIGGLALTAVIGCTPKPSQEQLQALDRTCASATDA
                     ERALETKRRELSDVERQLGPKRQVLAERQRYLGEVRSNLQAMQ"
     gene            17954..18991
                     /locus_tag="PLT86_04315"
     CDS             17954..18991
                     /locus_tag="PLT86_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35874.1"
                     /translation="MNPIKTYALARIGLLGNPSDGYFGKTISCAITNFSATVTLEEGE
                     GISIIPHPTSDPFSFESLANLRETAIRQGYEGGYRLIYATCKKFADYCAKREIVLPPK
                     GFTIRYDTTIPRQVGLAGSSAIITALTKGLRTLFGITESQFPLEIQPSFVLSVEEEEL
                     DIRAGLQDRVIQTYGGVVYMDFDRELLLHRGYGRYVQLDPRSVPPLFLAYVNRPKDSG
                     KAHSDVRARWSRGDEDVILAMREFADFAEQGREALAQRDWAKLGDLMNRNFDLRLRLF
                     GEKVIGDQNLEMVKIARSLGAPAKFPGSGGAVIGIWKDGDHFERLREAFASHGYAFTN
                     VQVENGPQYPV"
     gene            19003..19875
                     /gene="prmC"
                     /locus_tag="PLT86_04320"
     CDS             19003..19875
                     /gene="prmC"
                     /locus_tag="PLT86_04320"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT35875.1"
                     /translation="MSIETPTTLLDYLTRATEYFAGRGMENPRLNAERLLCGVLGCKR
                     IDLYVEFERVLNPGEIATYREYVRRRGRNEPLAYILGTAAFCGRDFSVTPSVLIPRPE
                     TELLVEHVIPLLKTATPTFIADIGTGSGCIGITFALELPEAEVAMVDVSGEALEVARR
                     NAHEHGVHGRAHLVQGDLSHPLRAGTFHAVVANLPYVAAAEKNTLPPDVVEFEPHVAL
                     FGGETGLDLFEHLLADVRRVLVPDGVVALEVGQDQASVVAQMCQSYLPEGEISVHRDY
                     AHIERVVVVTGVNA"
BASE COUNT         3972 a         6085 c         5859 g         3975 t
ORIGIN      
        1 gagtgttgca tcttcttggc agccttggcg cgggcgggga ggaacgccag acgtctaccc
       61 ttgtcaaggg gttgtgcggg agtcggtggg agccaatcgt gtgcggcatg cgcggaggag
      121 cgcttgcgga ggagttcgcg cggcacgcac gaacaatcat actggagaag agacggaaag
      181 ttgaccttgc ggcgctcgcg cgtattcggg caattatgga cgaagttcgg cccgccgtgt
      241 tgcactgcca cctctccacc gccaatacct ggggcaggat cgcggcgctg atgagaccag
      301 aaaaacgccc tgctgtcgtt gtctctgaag gaggcatcga aacctggaaa accgtagttc
      361 acctcgcgat tgacagggcg ctcctcggcg tgacggatgc ggtggtcgga aatgccgaag
      421 ccatcgccca ctaccttatc gatcatgacc gaatacctgc acgtatcgtc cgcgtgattc
      481 cgaatggaat tgatctggcg cgggtccagg cgaaactcca cgcaacggaa gaagaacgcg
      541 tctcgcgcag gaaggaactg ggcctcactt cggatcattt cgttgtcggt catgtcggcc
      601 gttcctgtgc ggtgaaaggc cttgacacgc tggcggcggt gatcgagcaa atggttcgcc
      661 tgaaccccga tttccgcctc gttcgcgcgg ggcagccgcc tttgcctgac gaaatcgaac
      721 ctgcggaagc gttccgtcgt gacgtcgagg cgcggggcgt cgcagaccac gtgatctggc
      781 acccgtatac acccgacatc tcttccgtgc tcatgatcct tgacgcgctc gttcagacat
      841 cactcagcga ggggctgccc aacgctgttc tggaagcaat ggcgatggat gttcctgtag
      901 tggcaacggc tgcgggcggt acatcggaaa tcgttcggca ccgggagacg gggtggctca
      961 cggaaatcgg ggaggccaga ggactttgcg agggtcttga acacgtacga aatgaccccg
     1021 aagagagccg gctgtgggcg tgtcgagcgc gaacaatggt ggaacacgac ttctcgctcg
     1081 cacgcatggt tgagaatttc tgcgatctgt acgatcgcgt cgccgggctc cacccgtgac
     1141 cctcgcgtgc aacgcagcgt gtatccatcg cattcccgtt gacggcgcac cgccgcggaa
     1201 cgatcatttt tacggggcga acccgcatcg ggcttacttc acgctgatac ccacccggca
     1261 tcttcaccag tacgagttcc gcaatgggaa aaagaccaca gggatggagc atacgcgttc
     1321 cggctacgac ggcaaacctt ggcccgggtt ttgattgttt cggactcgca cttcagcggt
     1381 atctgaatct tttcgtcacg gttcgcgggg aaaagggcgc ctggcacatc acgttgcatg
     1441 gcgagggggc cggtattctg ccccgggacc gcaccaacct gatataccgc gcactcgtac
     1501 tcgggtctga ggttgatgcg gagtcgcttc ccggtatctc catccgcatg gaaaacgcca
     1561 ttcctctcgc gcgcggtcag ggttccagtg ccgcggcgat tgttgccggc atggcggcgg
     1621 cgcaattgtt gaccgtggga aagatcgatc cggaccagct catcaatcga gccacccttg
     1681 tcgaggggca ttcagataat gtggctgccg ccgtaaaggg tgggcttgtc cttaccaggg
     1741 aaagcccttc aggagttgca atccgtccga ttcccacccg cgacggcgcg ttatttgttt
     1801 cagcggtccc ggaattcgag ctgaccactc acacgtcgcg ttcggttctt cctcagcagg
     1861 tgcctttggg agatgccgtt ttcaacctgc gtgccgccgc ctggatggct tccgcgtggg
     1921 cagccggcga ctgggaggct gtcggcaacg cgatggaaga cagattgcac cagccgtacc
     1981 gagcgccgct gatcccgggg ttcgacgacg tatgcagggc tgctcgagcc gctggagccc
     2041 ttggtgcatg cttgtcgggt tcgggccccg cgatagtcgc gctggcgctc cactcatcgg
     2101 acgctgtggc ccgcgccatg gagcaggcat gggcaagaca tggcattacg agccgcgcgg
     2161 aggtgaccca cgtagacacc gacggattga cggtcgcgcc gctggaagcc tgaggatagc
     2221 cgcaaatggg acgcagaaag gattatcctc tcccctcttc ggttggggat gagaaaaagt
     2281 gggcaaaagg cgagtggtgg gaatggctgg cgatcttctt ctcgatcgcg gcgctgtggc
     2341 cggtaatcct tcgttgggat ggcatcatat gggatcttgc gctcttcggc gctctcgtgc
     2401 tgatgatcgt ggtcttcctc cgaagggcac gccgtatgaa gcggtcgtgg aagcaatgaa
     2461 cggcacgaca ctcctcggat gaacggatcc aatttcccct ctgccggttg ggaaatcacc
     2521 ttccgtgagg ggtggtggtt gtggagcgca ctcggtctcg cgctgatcct gctggctgtt
     2581 ttttcctaca ggagaaccat cccacccctt ccgccacggc gcagagcgct gctctttggc
     2641 ctgcggctgc tcgcgctttg tgcgttcatt ctcgcgattc tgaatccgcg gctggaacgc
     2701 cgggggatgg agccgcgccc gcggcgcatc gctcttctgg tcgacgaaag ccgcagtatc
     2761 gcgcgtgccg ggcaggaatc ggctgtgcgc tcaatccttg ccagcgcaca gatgaaacgc
     2821 gtgtcagctc tcgcggacgt ggtcgcctta ggctttgcgg atacagtccg cgtccttgac
     2881 gaagccacgt tgtccttccg cgggcccgcg acggaaatcg gtgaggcaat cctgagccag
     2941 cggcgcgtgt tcccgccgcc cgacgctatc attctcatta cggacggatc caacacctcc
     3001 gggtcagagc ccgtccgggc cgcacaggag gccgcccttc cgacatacgt cgtaggggtg
     3061 ggtaatccac accccgagcc ggatgcacgc attgttggtg tcgctgcgcc cccttacgcg
     3121 gtcgagggta aaccggtgtc cgtcaccgtt gcctgggaac atacaatacc cggcatgcga
     3181 gcctccacca tgcgcctttt tcaccagggc agggaaatca cccgggcaat tgttctgcct
     3241 cccgaaaccg gcgcaagagc tgacgtggat ctgacgttta caccctcggc acggggaatg
     3301 cagacctaca ctgtggaact tgagggcgtc cccggggaat ctcgcacaga aaacaacagg
     3361 cgcacgtttt cgctggacgt tgtaaaggcg aaaaagcgcg ccctcatcat cgcggggact
     3421 ccaagcgccg atgtcggctt ctggaatcgt ttcttcggcg cacgcgagga ttacgatccc
     3481 gcaatctggt acgcaacccc ttttcgtcgc gttgctccac tctccccgga cagtattgcg
     3541 gggacagacc tcattgtctg gctcgaccct gccgggaaca cactgcctcc tgtcactcag
     3601 gacgcggtag cgcgtgccgt cgaagaaggc tgtgggctcc tttgtgtccc cggaacgaac
     3661 agcgtgtcgc ctcgcctgcg tgaaatactg cctgtcgagt tgaccggcac caggttcgag
     3721 ccgggtcagt tcgaggtcag gcttgcggct ccgcttttcg cacaccccat ctcaggaatg
     3781 gaccccggat ttgcggaatg gtcttcctgg gaatcagttc cgccgttact tggactggtc
     3841 tccggactga ggccacgcca gggtgcaacc gttctcgtca ctggtgacgc tggtcccgtt
     3901 gccgtggccg gacacgcaaa aaaagggcgg gtcctcgtgt tcggaggggc ggggtactgg
     3961 cgctgggaca ttttgccccg gggaatgggg atcgagaatc cggcgggaac tgcgttctgg
     4021 aaggccgcca tccgttggtt gatttcaagg gaagcgtccc agatggtccg ggttcaaaca
     4081 ggacgcccct tctaccgcct cggtgagccg gtccgcgtgg acatattcgt ctccgatgag
     4141 gcttcaaagc cggtggacaa cgcgtcagtg atcgtggcca cggacgggcc ggacaactgg
     4201 acggggcggg caaccctctc cggtcccggc aggtacacgg tgtggatacg ggggctgctg
     4261 acgggcgcac ataccatgcg cgcagaggca ttctcaggaa ccacctccct gggaacctcc
     4321 gaaacccgtg tgcttgtcga cgcttcaagc gcggaagatg acatcgtgca gatgcagacg
     4381 gatgtcctgt cgcggatcgc gtccgcgcat ccaggatcca gattcgtcac cgcacgggat
     4441 gcggagcagc tattggcatc gatccctcta tcccccgttt ttgaatacag cgaacgcacc
     4501 gtttcgctgg gctctgccgg gtgggttcta tgtggcatca tccttttgct gacaggggaa
     4561 tggggtttac gacgtacgca cgggctgctc tgattctggc tacgacgtgc acaacgttct
     4621 cctctcctgc ggaagcccgg cgcggcggac cggagtcctg ccccctggta atctccgaaa
     4681 tcatggtcaa ccccagcggc actgaaacct ctcgcgaatt catcgagttg ttcaacgtcg
     4741 gaaccggctc ggtcaatctc ggtgactacg ttgttggcga cacaaacgag acggacgcga
     4801 ttatcgcggt ctcgtccggg ctcgtcctca aaccggggcg gttcgccctg atactggatc
     4861 cagggtattt cagcgcaaac gagccctacc ccgacattcc ggctgaggct cttgtcgtta
     4921 cgatcggaga cgccgcgttc ggacgaggag gcctcgttaa cagccgcgcg aacacggttg
     4981 tgctccgttc ttcgggcggt tgtgaacaac gcgtttcgta ccttccagag gccgtgttcc
     5041 cggggctttc gctggaaaag attgatgtgc ggggacctga cacgccagac aactgggcag
     5101 tcagccttgt gcccgatggc acaccgggcc gcccgaattc gcgtcgcagg aatgcgtgcg
     5161 acgtttccct caggaatgcc agcgcaaatc ctgatactct gcatcctccc gaacagctta
     5221 ccgtacgatt tgacgtcctg aactctggaa ccgacaccac agggccattt cccgtcatat
     5281 tgttcgctga ccgcgacagg gactctgtcc tctcagccgc agaggacacg ctcgtccgtt
     5341 ggaacgtgag gtctctgcaa ccagccgaga cgttcgccgg ggtagaaacc ataccggtgt
     5401 cacgaccatt ttcgggcccg ctgatgctcg ccgcaatgct gccgggagac gagaacccga
     5461 gggacaacac gcaggcacta agatgcgtca ttgggctccc agcccgatcc atcgttatca
     5521 acgagatcat ggcgtatccc tccgacggcg aaggccagtg ggtggaagtc ctgaacgctg
     5581 gagccgaccc tttgtcactc gcggggtcct tattgaaaac tgctacggga agcggttcag
     5641 cggactacgg cccgttgttg tatccgggag attaccttgt gattgccgca ttgccggaag
     5701 gcgtccggac gcgatacgga atcggcacac ctctgtgtgg cgtgaccggc ggttttccgt
     5761 atctcagccg aagtgcttcg tcgtcgcggc gcgtgagttt tctggacgcg acaggcacct
     5821 ccatcgatga agcacattat cccgttccgg aacaaggccg ttcactcgaa cgagctgact
     5881 ccgccagcga cggcagcaac cctgcatcat gggcaccatc gtgggcgccg gcgagggcaa
     5941 ccccgggact acgcaacagc gcatcctacc caccgccccg agggttcgat tgcatggccg
     6001 acccaatacc cgcaacggac accgtcggga tcaggataat tacacctgag cacgggtctt
     6061 cggtctcgct cactgtcttt gatatggccg ggcgggagat acggaccctg ctcacccggg
     6121 agcgccttcc cgcagaatcg cacgtccgct gggacggaac ggacgggagc ggccgtcaag
     6181 ttcccactgg tgtgtatatt ctccacatcg aggttgtttc acccgacgaa ggcaagcatt
     6241 tccggcgttc gcaaccgttg gtgatcgtcc gccgcagatg accgatggag aagagaccgc
     6301 ctttcgtcgg caagaacgta cctgttggta cgttgtgtac gccgggcgaa gacccgcact
     6361 ccacacgtgt tgcccttgag aagggctgtt tcctgcatcg ctggccgccg ccgttttgcc
     6421 ggcacgggag ttgcggcagg attgtcccga aaggggcagg agttgaatga gatgagaatg
     6481 cggagccttt tgcacgttgc cgtcatcgcg ggtttcggga tattcgtctc tccccgcggt
     6541 gcggtttcgc agggcggagg tggtcctcag cagggccgta ttgcgggaga ccctctgccg
     6601 gcgctggcga cagaatggca gcagtccttc gacgcagcac gtcagacaaa caacagcgaa
     6661 gcgctgtctc ttttgtcacg agcgaaaacg ctgctgaacc aggcagaaac ctttcggcgc
     6721 aggggtgaaa ccgcgcgcgc tgcggcaaac gctgacgagg caagactcct gattcttcgg
     6781 gcaaaccaga tcctcaggac cccgccaggt cgttcccccc agatagatgc cacgttgcgt
     6841 gatgccgaga ctctgatgcg gaatgcagtt gcacaaggcg ctccgcccga ccagacggcg
     6901 caagtgaaat tgctgctcga tcaggcgcgc aaggcgcaga ccgagggccg ccatgacgcc
     6961 gcgttgcgac tctccgccac cgcccagact caggcccgtc agctctggca gcgcgcggta
     7021 gcatcgcgcc agctcagaca gcgatgcaga gtactggaat ccgccgctga gccgatggtg
     7081 cgccgcgctc gcacgatggc cgacgccaac gggggggatg cggtgaagag cgctgcggaa
     7141 agagcacagc agtttcttca gatggctcag agcactgatg aagcaaccca tccggccgca
     7201 aaagcccgac tccttgaacg ggcgatgcag aatgctgaag cggtgctcaa aacgcttgat
     7261 gccgcaacgt attccgccca tctcgcgtcg cgaagcattg ctgacgcgga cgcggcgctt
     7321 gcgcgggcgg acgaagtcgc gtccggagac gccagcggga acgatgtgaa cggagaattg
     7381 cgccgaggcc gtgtggtgat cgcgaacgcc cggcaggaac tcgcatcggg aaactcgatc
     7441 cgagcgagag cacttgcgga agaggcgcgt cagatcgccg aacaggttct gcggagcgca
     7501 ctgggagaag taacggaagc gtcagtacgt gcggcactcc agcagacgga tgctttgata
     7561 gaacgcgcct ctgcggcatc ggggaacgag acagatccgt tgctcgtaga ggcacgaaac
     7621 cgacaggcag atgcgcgacg catgctcgcg gacggagacc ttcgcagagc cctcgcccaa
     7681 acacgaatcg ccgctcgcct tgcccaacga atcaatgctc gctgacacga tcgcgtgcgg
     7741 atcaccgcgg cccgggttca tggcttcgtc cgcaaagcgg cttctccgcg ctggatggag
     7801 ccttcttgtc gcgatcgtct gcgttagtcc ggcgtgttct ccgcctgccc gctctcctga
     7861 tgtgttcccc gacagcagaa gcgatgaaag cgctcttgag gttgcttcgg acttgccggc
     7921 ttcagccaat ctggacgagg atttcgctct ctggcgcgag acgtacgagc tgacgcgcag
     7981 cgcctcgtct gattccatct gcatcgcgaa ggcggatgcc attgcgtctg aagcggaaac
     8041 gcagtggctg ctgggagatc gggagcaggc cgaggtgctg tggggtgagg cgtttgacct
     8101 cttgtcaggc acgtgtgcag acactatcct agtggagccg gttcaccccc ctgattcccg
     8161 gtagacacgg gcctgctggc cgttctggat tatccactcc aagggcgcgt ttgtggcaca
     8221 accccacacg agtctcattc gcctcctctt tctggtcgct tttttgccgt caggcccgtg
     8281 tgttgcgcgc gcaggcgatt tgtgggagac cacatacgaa ttgcacgcag ccacggaggt
     8341 ggcgggagaa gggtttcgga ttgtggagtc cgagaccacc gaagtaacga cggacgcgta
     8401 cggccaactg cgcgtggtgt cgtccggtgc tcttggctcc tggaggaaca tcattctgga
     8461 caaccggttt gccgcgggaa tgagttcact tcggtacgat ttccggggaa ccggggaatg
     8521 gaagaacacc tcgccggttg gcgtcaacct ggacggagca gtcagcgcca ggcgctttca
     8581 cggcgcgaac agcgcgtcac gaagttcgta catcgaaacg tcaggagagg ttgccgctct
     8641 ctcgccactt ccccacgcgt accccggaac ctgggggtct gttcattcgg ttcggggagt
     8701 gtggtacgac cggacctcgt ccgtgtacac aaacgccatg tcgcactggc acggtctggc
     8761 gctggaaacc acacgagatc tcagcacatg gaccacgaaa gccggtgtgg aacatgagtt
     8821 tgttccagat tcgtccgaac tggggtactg ggggttctcg ggcagttcct tcgcgatgtg
     8881 ggtgccttca ctcgattggc tggtatccgg tggtgtgatc gcggcgtgga aagactaccc
     8941 ggcgccgggg acacatcccg acgagagctc cgcgacatta catgcacgtg tgacgagacg
     9001 cctgaaccct tcgtggtcgc tggaaaccgg catcaatggc gcggtcctcg ccttcagccc
     9061 tgcggacgat gtgttcccca gcacccgtaa tatggaactt cttgccgaag cgaagtggga
     9121 acccggcctc gtcagcacgc gttttggagc gggcgcaggg atgcagcaat cgcccagttc
     9181 cctggcagac gagtactccg agcgtttcct tcaagccggt tgcaacctga tttcgcgcac
     9241 ctccggcttc tctgatgtga gtatccgcgc aggcaaaagg acctatcgac atccttcggc
     9301 agcctattcc agcgactatg tatttggcga gttcaccgca cttgtcagcg gagcacgcgt
     9361 gttcggggta cgcgccgagg ctttcgtgac gttccgttcc gagcaccacg aggacgacac
     9421 ggagaattcg aggaatctgt tcatcaccgt ggacatttcc cggaccatga acacccgtcg
     9481 atgaagtgca aagagccggt tgagggggaa tggagtattt tggcgcgcgg caggtcgggt
     9541 accgctcgga tcagttccct ggatctgctg tcccggcgtt gttcgcgccc ttgcggagga
     9601 gcggaggaag gagtaagagc gccagaagaa gctgcggaat cgcgtatgcc gcgttgtagg
     9661 cagcgctgta aatccacgca ctctccccca gaggagcata cgcggagaag aagacggcgc
     9721 cggagattac gtgtatgccc cacttgagga agctcgccag gctcacgccg agagccacgg
     9781 aggtgagtgg aaggcgtgcc gcaaccagcg caattcccgc caacgccgcc gcgagcagaa
     9841 ggaaattgcc caaccactcg ttctgtttgg cgcggacaac actcagcccg taatccaacc
     9901 agtctcgcgc agtctccccg tgagcggtgg ttacccccac cacagagctt cgtcccgcgg
     9961 cgctgttccg gtacgtaacg attttcgtct gcagccctcc gagaatcgtg tcagcgctcg
    10021 cccacatcac gatgcgcgaa tctggagatc gctgcacctc gatctcgtcc gtgcggctta
    10081 cccggcgcaa ctcgaaccat tggagccccg ccacaaatac aaccaccaca cccagaaaaa
    10141 cggtaaggcc aacgcgaacc gaacgattgg aattgaccgt tcggaatacc ccggccacgc
    10201 cgcaggcagc catcgcaagc gggtaatcca gaacaacctg cactgggtgg acaataatcg
    10261 ggcgcagaag caattgcact atgccggaaa cggccccggc agccatcgca gccccagtcc
    10321 caaaccggaa tgcgacgtac agaagcggga tcgcttccag ggtaactgat ccgccctgcg
    10381 gcatggaaaa aagccgcagg aaactcagag caacgctcag ccccgccgca accgaaatga
    10441 gaacgagttc acgcgtccga atcgtcaaat tccgttcccc gtgcatcgtc accgtgaatc
    10501 tcaatatcaa ccgtggtaat ccgctgggcg tcaacacccg tcacggtaaa cgtgtaggga
    10561 ccgtaacggg tgctttcccc ctttgacggg actgctccaa actggtgata gagaaaaccc
    10621 gcaactgtct cgtaaccctg ctcttccggg aactcggccc cgaggagctg cgccagatcg
    10681 gcaatactca tcccggccga tacctcaagt ttcttttgat ccagccactt gtacggttgc
    10741 tcttcttcat cgtattcgtc ctggatttca ccgacaattt cctccagaac gtcctccatg
    10801 gtgaccagcc ccgcggtacc tccgtattcg tccacgacga tcgccatgtg cttcttctgc
    10861 ttccgcagct ccctcagcaa gtcgtcaacc cgtttcgtca tggggacgaa cagcacatgt
    10921 gattcccgca tcgcgtcttc cgcggtggcc gtactcccgt ttgcgccacc ggaagaactg
    10981 acggcgtcga ggacgtcttt cgcgtggacg atgccgacga tgttgtcgag tttgccgtcg
    11041 tacactggaa tacgggaatg ctgggtctcg cggatcaagt ccaggagctc gttgagcgat
    11101 tggcttcgtt caatagccac catgtcaacg cgcggaacca tcacttctcg aacaagcgtt
    11161 tcatcgatac tcatgattga gctgatcatg tcacgcttgt cagcctccag cacgccgtct
    11221 acctcgtcac tctccacaag tgccagaagt gcttcctcac gcgcctcgcg gttttcgggc
    11281 tgatgcgcga gatgcgtcag aatcttgtcc agaaacaatg aaagtgggaa cgtgagcacc
    11341 gcccacgcga caaggagcgg cgcggcaaac cggaggagag gcccggacat gtcatcggct
    11401 gtcttaaggt tcggaaccac aaagtgagtg acgaccagcg gaacaaatcc aaggagagcc
    11461 gcgatagcgt acgcgagaat cagaccggcg ccggaaagcc ccggaacgct cgccagaaac
    11521 acaaaaacgc ccgcgataag gatgagatct gccgcgagga caccgatccc gaaagagatt
    11581 gcagcccggc gtttcgccgg ggacttaagc agaaactgcg gaacctgcag cctgttcgca
    11641 tcaacaagcg cctgaacgct ggccacagac cgcagcgaaa gaaaacgttc cgcccgaccg
    11701 aacacccaac tgaggccgaa caggaacgca taccctatga gaaaccagat actgaatgag
    11761 tcatccacga cggtaaaact gttcctccct gtaagtcggc atcaggcgca ttaccacgca
    11821 agttcacgca gatagcggcg cgtatgattt gccatacgct tcccatcagc cagtgtgtgg
    11881 tgatcgtgcc ccgccagatg cagcacaccg tgtaagacga gtctgacaat ctcctggttg
    11941 aggcctgtgc tgtaggtacg aagttgcccg gccgcatact ccggacacac gtagatctcg
    12001 ccttccacgc gatccgcgga gtccgccaga tcgaacgtca ggacgtccgt caccgcgtcc
    12061 ttgccccgga aactcctgtt caactcgcgc atgcgctccg gcccgacaaa aacaatggcg
    12121 accgatgcgg gtacgtcgcc gcgctctcgt ttccacactt cacccgccat tttgcccaag
    12181 ccagccttcc acccccgttg catcccgcgg aggtgaggcc tcgccggcgg gtcaacgcac
    12241 aatctggcac gcacttgtgg ctgttgcgag gcacacatga gtctgctcgg acgggactgt
    12301 aagaacggat gtcggtatca aaaagatacg gaaaaagaag ggtgagttgg gcttcagatc
    12361 cccctgttgc ccgagttgcc gtggtgttcc gaagattccc cgtgcgtgtg gccggttgca
    12421 gcctcttcct ggctttcttt caccagaggc tccggtgtga tcggttccgg gtattcgatg
    12481 cgcttgtgca gcgcgcccgc cagaacgggc aggaaactct tctcgatgag atcgagatcc
    12541 cgcagcgtga gcgcgcattc gtcgaattgg ccgtcatcca gacgctcacg aatgatctgg
    12601 tggaccgtat tctgaacatg ggctggcgtc cgctcccgaa gcgacctcac cgccgcctcc
    12661 accgcgtcgg ccatcatcag tatcgcggct tctctcgtct gcggtttggg cccatgatag
    12721 cgaaaggctt cctcactcac gttttcctcg ccgtacatgc gctgcgcctt gcgatagaag
    12781 aacgtcatca acatcgttcc gtggtgttga ggaatgaaat cgatgatctg ccttggcagc
    12841 cccacccttt ctgcgagatc aatgccgtcc ttgatgtggg cgctgaccac gagagcactc
    12901 atgtgcgggg tcaggcggtc atgtggattc cgcacggtaa gctggttctc gacaaaatgg
    12961 tccggccgca acatttttcc gatgtcgtgg tagtacgccc cgacacgcgc cagaagcgcg
    13021 ttagctccga tcgcctccgc agcactttcg gtgagattgg caatgatgat gctgtgggtg
    13081 tagctccccg gcgcacggag agaaagttcg cgaagcagcg gggcgttgag gtcagagagc
    13141 tcgagaagcg tcatgttggt ggtgatggaa aacacctttt cacacaccgc aagcaacccc
    13201 attgcgagaa tcggaacggt gaacccgttg accgcgccgg gccagacatc cttccacatc
    13261 gctttggcgg tggtgtcttg gatcaaatcg atcgcaacgg tcgtgaacgc gattgcgagc
    13321 ggcaaccaga gcatcgcgct gagaaactgg tttcgattgc gcacgttcct caccgagaag
    13381 gccgccacga aacccgcaac aacgttgaca atcaccacgc ggtactcaaa cccgagcatc
    13441 gcccctccca gaagggatgc ccccactacc agcacaaggc cgacctcaac accgaagagc
    13501 agtgtgccca gcatggccgt gagagcaagc gggacgatgt acacgggcag aaacgggttc
    13561 tgtgcaacga aagaagctgc aagcaacggt atccacgcga gcaggatgca gagaaacagc
    13621 cacgccggtc gcgtccagat gggcgggtga aacgttcgaa ggtacaccgc aactcccacc
    13681 atcacgagca acacgatgcc cagctgccca gccacttcca agaatggctg ccagccgtac
    13741 gcctgccgga ggcgcgcccg ctgcgctgaa accagggagc ctacttcatc aatgtcttcc
    13801 gcggtcagaa cggcgtggct ctctatgaac cgctcatctt tgaacaccgt tcgcttgtaa
    13861 atcggaaccg ccgccatcac ctgttcctgt ctgtcagcgg tctccgttct gtcatatgcc
    13921 agattcggtt ccaacgccgc ttccgccacc gcgtacaacg cgcgcagacc atctgatgaa
    13981 agctgaatga cgttctccat agcgacacgg gtgatcaact ggccgagttc gtcacgggct
    14041 tcttcggcgt tcatcataag cccaatgggt tgctcgtact caagcgtgtc accgcgtatc
    14101 accgttacaa cgtcggagga atctcctctg gcggcaccgg cctcaacctt ccgttggacg
    14161 atctccttga ggaattgcca gtcctccacc ggaaccacgc cacgttcata cacccgctcc
    14221 aggacgggaa cgagcacggt tctggcaata ccgaggtttc tgccgccgct cgctttcaac
    14281 cattggattg cgttccggtt cggacgaatc ccggaaacag aaacggtgcc ggcagcgggc
    14341 gagggatttc cggcaagcga atcaagaaaa cgctcaacac gccggcggtt ccgcaatccg
    14401 acgagagggt ccatacgcat gaccggtggt acggaattcc tcgcttttct tctttctgtt
    14461 tcgagttcga gggggttttt tctcacattg aacgagaaag gagcgatcac ctcccgcgga
    14521 gccaccatac cctcgcgcca gctactgtat tcattcatcc gctcgcgggg aaacagcgcc
    14581 atgaagaggc cgataccgac cagcccgacc agaatgcgca gatacttgcg gtactcctcc
    14641 gtcaaccgcc cccgcaagga tcggcggagg cgcacaagta ccccatcgtg gcggatgagc
    14701 cgttgaaggt ggtctcgcat tccgcgggcc cctcatacgc cctgttgatt cgcgttgttg
    14761 ctggcgccgt gtgaactttc cacacgatca tacgcacgga tgatcaactg aacaagcctg
    14821 tggcgaacaa cgtcacgttc tgacagcgac acgaaggata ttccggtgat accggaaaga
    14881 atctgtttcg cgtcgatcag gccactggga acggcggcgg gcagatcgat ctgcgtgatg
    14941 tctcccgtta tgactgcctt tgagtccgcc ccgagccgag tcaggaacat cttcatctgc
    15001 cgtgtcgtcg tgttctgcgc ttcatcgaga attacaaacg cactgttcag cgttcgcccc
    15061 cgcatgaacg ccagtggcac gatttcgatc acgtgcgtct cgaggtagtt cttcagtttg
    15121 tctggcggaa tcatctcgtg caaggcgtca tacagcggcc tcagataagg atcgaccttt
    15181 tccttgagat cgcccggcag gaacccgaga ttttcccctg cttcaactgc gggacgcgca
    15241 agaatcacac gcgtcacctc tctctttttc aatgccgcta ccgccatggc aactgcgaga
    15301 tacgttttgc cggttccggc cggtccgatc ccgaaaacga tatcgtgccg cgcaattgct
    15361 tcgcagtatt ccctttgccc gcgggagcgc ggactgatgg gcaccttgtt ggtggtgtgg
    15421 agaatcaccg gccctgccgg gctccccgta ggaggatgcc cctcccccga gcgcttcttc
    15481 gacaatgcta ccggggaggc agttccaggt cccggagcta caaaatcccc tctcaggcgc
    15541 tggtcgagct ccttgactgc cgcggccact tccgcttctg ttcctcgcac cagcaactgc
    15601 ccctggcggg aaacgaggga gacatggaac tcctcttcca gttccttcct gttcctgtca
    15661 agcggaccga aaaggtgctg catgtccgct ccttcgatgt cgatcgactg agtcagtaac
    15721 ggttgtgctt tggctcccat gaagttctca cgcgtcctcc ccaaaaaagg aagcccccgg
    15781 gctccccgca ggagccagag ggcatccgaa ccacatcatc tccgccggat cacaaggcgc
    15841 gagtctcgtt cgtcagttct gcggaatcgt caaaatcatg ttcgggtaga tcaccctgcg
    15901 cccgccgctc accgcctcta tgacgtcccg gtttgcacgg tagattttcg tccactgaga
    15961 cgggtcgccg tacacttcct gggcgatggt ccgcatccgg tcgccgcgtt tcacccagta
    16021 ggtcccgggg gcctggttac ggggtacgcc gaggacccag ttcggataga tgagatccgg
    16081 gttccgaatt ctgtctttgt tggccgtgta cagacggacc caggtgtagg gatcgccata
    16141 gatttcaggc tttttggcga ttttccacag ataatcgccc cgaacaaccg tgtactggtc
    16201 acgtcgaacc tcgggaaccg gacgctgcac ggcacgcagt tccgcaatca tctgatctgc
    16261 tgtctggatc ttcgccttcg cttccggcag ggcggcaatc cgattggtct tcaactgcgc
    16321 caactgctct gcgattcggt cgagatcacc ccgttcccgc gcgtccacaa tctgatttgc
    16381 ggggagctgg cgcattgcct gcaactgctg gattatcccg tcgagctgcc cgagataggc
    16441 gcgtattgct gcgctgtccg ctgcaacggc ttccaaaact tgccgattgg tggcggcaat
    16501 ctgctcatcc agcgatgata actccgcccg cttctgggca atctgctgct ccaccaccgc
    16561 gcgcgcgctg tcggcggccc gttgacggtt ctggcactcg cgcaggcgcg cttcgtattg
    16621 ctgcatcgtc atctgcggtg ctgtctgggc tgcagccatg gcagacaacc ccagcaacgc
    16681 gcccaaagcc actgcataaa gcgttcgagt catacctgtg acccccttcc cgtgttcacg
    16741 gggtgagtat ttcacggtca taatcccttg attgaaaacc atttcagtgt cattccgcac
    16801 tgctttcgcc acccggcgca gtggaatcgg ccggagccgt ctccgcatcg ggagcgggcg
    16861 ttgtttcact ttcactcgtt ggcggcgcct gctcttccgc cgccggagca acattttcct
    16921 gtggctcggc cgccggcgcg acaccttgct ccgactcgac tgccggagta acgctttcct
    16981 gtgattcggc cgtcggcgct gcctgttcaa cagatgccga atcccccgga gcagtcacgg
    17041 cggggacgac aggctgcgtg gtttccgccg gagcagcctg catcggtgcc ggctctactt
    17101 cccacgtggc gagttcctcg tcggacattc tgcctgtcgc gatgcgcgaa cggattctct
    17161 gcaattccac cagccttgcc tgctgtgccg agaaatccgt ttgcgatgca cggtacgccg
    17221 ccatcttcct ggcagcggag gtctccgccg ctgctgcttc caggcacgcc cgttcggcca
    17281 gagcacctgc cgccggacgc gcacccggtt gagcactagg cgcacatccc gcaaaaagcg
    17341 cggtgccaca ggcaagaacg cacacggcag cccgacggac gatcatggca aactcgctta
    17401 ctgcatcgcc tgaagattgg aacggacttc gccgaggtac cgctgacgct ccgcaagcac
    17461 ctggcgcttc ggacccagtt gacgttctac gtcagacaat tctctgcgct ttgtttccag
    17521 cgcacgctct gcatcggtcg ctgacgcaca ggtccgatcc agcgcctgca actgctcctg
    17581 actgggcttc ggggtgcatc cgatgacagc cgtaagtgca aggccgccaa tgagcaccaa
    17641 accccaaggc gcacgtctca tacgatttcc tcccgtctgg cactataccg aaaacggcac
    17701 gattctgaag atgtgagcaa accaactcga ccttaacata cgcatgagtg cgagattcgt
    17761 caatgcccat cccgaaccgc tggggctttt ttttgtacta cggcgccgca cctgcgtttg
    17821 ttccgcgtat ctttgatacg acacgtatca tttgatacga aacgggccac gagaaaacaa
    17881 ccacgagcct attcaggcga aggcaccaaa ctttgagaaa atgcgacacg cattgcccga
    17941 aaggggaacg gcattgaatc ccatcaaaac atacgcactt gcaagaatcg ggctcctcgg
    18001 gaacccgtcc gatggttatt tcggaaagac gatttcctgc gcgataacca atttttcggc
    18061 cacggtgacc ctcgaggaag gagaagggat ctccattatt ccccacccca catccgaccc
    18121 gttttcgttc gagtcgcttg ccaatctgag agagacggca atccggcagg gatatgaggg
    18181 cgggtatcgc cttatctatg ccacgtgcaa aaaattcgcc gattactgcg cgaagcgaga
    18241 aatcgttctg ccgccgaaag ggttcactat ccgctacgac accacgatac ctcgacaggt
    18301 cgggctcgcc gggtcgagtg caatcataac cgcccttacg aaaggccttc gtaccctttt
    18361 tggcatcacc gagagtcagt ttccgttgga aattcagccc tcattcgtcc tcagcgtgga
    18421 agaagaagaa ctggatatcc gtgccgggct gcaggaccgg gtaatccaga catacggcgg
    18481 cgtggtctat atggactttg accgcgaact tctgttgcac cgagggtacg gccgatacgt
    18541 gcaactggat ccccgatcgg tgccccctct cttccttgcc tacgtcaacc ggcccaaaga
    18601 ctcaggaaaa gcgcacagcg acgtgcgggc gcggtggagc cgcggcgacg aagacgtgat
    18661 tctggcgatg cgcgaattcg ccgacttcgc ggaacagggg agagaggcgc ttgcgcaacg
    18721 ggattgggcg aaactgggcg acctgatgaa caggaatttc gacttacgcc ttcggctgtt
    18781 cggcgaaaag gtgatcggag accagaacct ggagatggtc aagatagcac gatcgctggg
    18841 agcccctgcc aagttccccg gttcaggtgg cgcggtgatc ggaatctgga aggacggcga
    18901 ccattttgag cgtctccgtg aagcctttgc cagccatggt tacgcgttca cgaacgtcca
    18961 ggtagagaat ggacctcaat acccggtctg acccgcaacc ccatgagcat tgagaccccg
    19021 acaaccctgc tcgattacct cactcgcgca acggagtatt tcgcgggtcg cggtatggaa
    19081 aatccccgcc tgaatgcgga gcgtctgctg tgcggcgttc ttggctgcaa acggatcgat
    19141 ctgtacgtcg aattcgagag agtcctgaat cccggggaaa tcgcaacata ccgtgagtac
    19201 gtgcgccgtc gcggcaggaa cgaaccactg gcgtacatcc tgggcaccgc ggcgttctgc
    19261 gggcgggatt tttccgtcac cccttcggta ttgatacccc gtcccgaaac tgagctcctc
    19321 gtcgaacacg tgatccctct tttgaaaacg gcgacgccca ccttcattgc tgacatagga
    19381 accggaagcg ggtgcatagg catcactttc gcactcgaac tgcctgaggc agaagttgca
    19441 atggttgacg tgtccggcga agcgctggag gtcgcccgga ggaatgcgca tgaacatggc
    19501 gtacacggtc gcgcgcattt ggtccagggt gatctgagcc atccgcttcg cgccgggacg
    19561 ttccacgccg tggtggcgaa tcttccctac gtggccgcgg ccgaaaagaa cacattgcct
    19621 cccgatgtcg tcgaattcga acctcacgtt gcgctgttcg ggggcgaaac cggtctcgac
    19681 ttgttcgaac acctgctggc tgatgtgcgc cgggttctcg ttccggatgg tgtggttgcg
    19741 cttgaggtag gacaggacca ggcgtccgtt gtcgctcaga tgtgccagtc ataccttcct
    19801 gagggggaga tttcggtcca ccgtgactac gcacacatcg agcgtgttgt cgtcgttacc
    19861 ggggtaaacg cataacgaga attgcaccgt c
//
LOCUS       DAOSOO010000036        19694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2753_length_19694_cov_6.678497, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000036 DAOSOO010000000
VERSION     DAOSOO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_19694_cov_6.678497"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PLT86_04325"
     CDS             complement(<1..1330)
                     /locus_tag="PLT86_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT35876.1"
                     /translation="MLTITEPSRTVPIVHEADICVVGGSCTGVFAAVRAARLGASVAV
                     VELGGFFGGVATAGLVNVWHSLKDVHDERQIIGGLTQETIDRLHRRAAVEFGTSPHAA
                     YFLNTEELKIELDNLITEANVRPFLHARFVAPVVADGRLDAVIIEDKSGRRAIRAKYF
                     VDTSGDGDLIHRMGLPTRFLGDIQPPTACCIIEGLGRVEEANPGFKLNEAVFKPEFPE
                     ALKLGFLWAAWVAGSRNMKMVAGTRVANADCSDADQLTAAEMEGRRQIRAMVDILRNH
                     FARGEEVFLRGVPSYIGIRETRHAKCLHSVTEMELLTGHAFEDTIGCGTYHVDVHHSE
                     KPGLTFRNLDGTEEWVVPGRPPVRGRWRPEGEDTPAFYQFPYRSLVPEGSKNVLVAGR
                     LIDCDRGAYGALRVMVNCNQTGEAAGVASTLALRENGAVADVNSAKLRNAM"
     gene            complement(1468..3501)
                     /locus_tag="PLT86_04330"
     CDS             complement(1468..3501)
                     /locus_tag="PLT86_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT35877.1"
                     /translation="MPAPQSHLDLYVSPLGRDNWSGRLAEANADDTDGPFATVTKARD
                     TIRELKRATKLPASVTVWLRGGRYILKAPLTFTHADSAPVTYAAFPGETPVLDGGTRI
                     TGWQKESPNGKEVWVTTIPEVAEGKWYFRQLFVNGARRSRPRLPKEGFYRVADYERPH
                     AEGPWGDIFMGADAFQCTPGHVQSWKNITDIDIVVIHWWVEERVSMAGFDPATNTVKL
                     ANKTGFTLKDDVADGLARYYVENVKEALSDPGEWYLDRSTGKLTYVPMPGEDIETAQV
                     FAPRLRKLVQVTGDADAENYVEFLRFEGLTFEHAEWDYCLSNQAAHKTPAVITFEAAR
                     SCAFENCTVRHIGGYAVELRDGCQNIRVVGNTLVDLGAGGVHLNGADALGPRARRTGN
                     NRITDNVIGEGGRVFLAAVGVLSRHSFGNHISHNHIYDLYYSGVSCGWVWGYHENVSQ
                     NNRIEKNHIHDIGHGILSDMGGIYTLGVQPGTMLRGNLIHDIEKSNYGGWAIYMDEGS
                     SHIISENNICYNTSSQGFHQHYGRENVVRNNIFAFGREGQATVSRYEDHLSVTFDRNI
                     FIGDNQPIFAMGRPEQRIFLSNNNLFWDVARAPWCGASTRNEKGEWGYKQEYSFEEWR
                     KLGYDPQSIVADPKCRDFAARDFTLEPASPAFALGFEPIDMSDVGPRPPEKRE"
     gene            complement(3553..5097)
                     /locus_tag="PLT86_04335"
     CDS             complement(3553..5097)
                     /locus_tag="PLT86_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35878.1"
                     /translation="MKSVIHSNRPPFRLASNVIYFHDWRYVDHGSHQWLTETGKGVPL
                     FTTESLPPLRYRCVDMPYGIRLVAQKAEKGQPVITPEMARAVFLFGGTLIHDEGVYRF
                     WYECWPEEGIGTPHMGVRNHVRYAESDNGVDWRFPDVGVANYRGDTRNNIVYGAMLRD
                     DDVGYHGGSVFKDPSAPPEERYKGFHLGHIPDDMYADYLRERPDAVDPYHRNQGLFGA
                     VSPDGIHWKPIREPLVAHYSDTHNCCTYDVQQGKYVAYCRNWFFRRRTIGRMETDDFR
                     RFPLPEDLFWPGATMAPHDLWYSQPKTLMPGTIDYHVMFPMRWSLATDSFHFHLATSP
                     DGVVWGFVPDASGYTPGEGVCSPGCAGEWDGGVVTPGIGLVDLPGNRTGMLFAGSPVP
                     HKHPRKPPLGKIAWATWKKERLVALECPMEGFFATQPLVFEGRTLRLNAKTQFTGFVE
                     VEACKPDGSPIPGRSFADCDRFNGDETDHPITWRGESDIGHAENAEVRFRVRMRSADL
                     FAMRFV"
     gene            complement(5152..5754)
                     /locus_tag="PLT86_04340"
     CDS             complement(5152..5754)
                     /locus_tag="PLT86_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT35879.1"
                     /translation="MEQAPAVAFLLQMPELFLVTGERNRTTGMHEAATARFAGPVTAA
                     EVGGNMEPLRVFVVDDDEAFVQLVTAVLSAKFGHWVASETDAVKAVERIKAEQPDVVL
                     LDLVMPRQDGYETLKFLLADEKTRDIPVVMLTNFDADKFRSMAERWGASAFIGKKAIG
                     LSAFLNRGAQADDLPFAQKDDLNYAQLDEVIRKAVANRSV"
     gene            complement(5898..7451)
                     /locus_tag="PLT86_04345"
     CDS             complement(5898..7451)
                     /locus_tag="PLT86_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT35880.1"
                     /translation="MRLTIGGKVLLGNLGMVALMCAAVLWVVRDLRRMDDLVELTVNA
                     QEQIIRINRISDDINEILYRGGIKVSMFQVGAFAASRDADEMTRSLRSRRAHRDSLLA
                     EVSRSRFGQRPALKYSLDSLLIACRKLDLLFESVELHFERGQPGRAERAFSQLHEVDA
                     ELSRAMTSLQEHAQEDANVLLAAASRFREHLILRAGVVLAVLLAGMLAMVMYLNRTIK
                     RATAGISNALSAVASGDFQQRVELVSKDEFSEIAEGLNATVERLGELDALKADFLSKV
                     SHDLRTPIAAVKQAAELLNDGIPSPLKEDQKEILQIIRTNAKRLGNLINDLLDTARLE
                     AGKLEIQPETTDLVAIIRRVATSIAPLSMEKRLRITLKAAPDLPPAYADPNRMEQVLM
                     NLLGNAVKFTPENGEIALSCEQEGAFLRCSVRDTGVGIPPEDLPRIFDKFHQVRMKRA
                     TKAKGTGLGLTIVKFLVEAHGGFVWAESTPGQGATFTFTVPVVEQPGEGEDVRYSGTR
                     EEDADVRPD"
     gene            complement(7464..8612)
                     /gene="dprA"
                     /locus_tag="PLT86_04350"
     CDS             complement(7464..8612)
                     /gene="dprA"
                     /locus_tag="PLT86_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT35881.1"
                     /translation="MSDRSFEELGDLLALCNVKGIGAARLAALVEAFGSPGAVLAANP
                     SELETVQGIPSAIALAIADMGRGRTDTVRDAVMRQCERIVRTNTSVIACIDADYPPHL
                     LETEDPPQLLFAQGDTGLLAIPGVAVVGTRRPSEYGRHITEAIAATLVRHGVTVISGM
                     ARGVDSIAHTVALDRDGLTVAALGCGVDVVYPPEARNLHARIREHGLLVSEVWLGAAP
                     DRINFPRRNRIISGLSRAVIVTEAPRASGALITADVALRQGRDVYAVPGDITRREAAG
                     ANELIADGARVVRDMDDLVISLGLGTRVPTPEGDQLGLPVSAPADLSPEERVVLDALA
                     LEPVHVDALAVKLGRKSEELLTSLTLMEMRGLAVAHAGSRYSRATMTS"
     gene            complement(8616..9656)
                     /gene="obgE"
                     /locus_tag="PLT86_04355"
     CDS             complement(8616..9656)
                     /gene="obgE"
                     /locus_tag="PLT86_04355"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT35882.1"
                     /translation="MEFVDTATIEVWGGDGGPGAVSFRHEKNVPRGGPDGGDGGNGGN
                     VLFVGDPRMNTLQEFRFRRHFRARHGERGLGSRWHGASGEDVTIRVPCGTLVFDAETG
                     ALIQDIVAPHEPVIVAKGGRGGKGNAQFATPTRQAPDFAQNGTPGEYRSLRLELKLLA
                     DVGLVGLPNAGKSTLLSVMSAARPKIADYPFTTLVPNLGIVSLAEHLTCVMADIPGLI
                     EGAHLGKGLGDQFLKHIERTRVLVFMVESIDPDPDATLKLLRNELREFNPDLLKKPWV
                     YVLSKADLSDAKPRKARGAHACFSLSAVTHVGLDRLRHELSVLLTETTDAGTDSDSRV
                     RFATRLPESPPQ"
     gene            9878..9952
                     /locus_tag="PLT86_04360"
     tRNA            9878..9952
                     /locus_tag="PLT86_04360"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9911..9913,aa:Glu,seq:ttc)
     gene            10043..10747
                     /locus_tag="PLT86_04365"
     CDS             10043..10747
                     /locus_tag="PLT86_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT35883.1"
                     /translation="MATQPFSLDAIRSVPLFTNLSDEMLEAVVKASTVVTFRRNQTIV
                     RENEEGNALFIILSGRVKVTLIRSDGKEAILAILRANDFFGEMALLDDQPRSASVVAL
                     ESTRALMLTRPNFIKLLEDDPQIVHNIMVALSGRLRKANQKIADLAFLDAIGRICGVI
                     LQMAEEAGEETDEGILIHNRPSHEQLGMMAGTTRETATHCMIALERRGYLLTQGRDLL
                     LFSPEDLKRDFLSPSS"
     gene            10749..11219
                     /locus_tag="PLT86_04370"
     CDS             10749..11219
                     /locus_tag="PLT86_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT35884.1"
                     /translation="MKLRIVVVGKPRRDVVEAFVADYLARLKPIWKTELVAVAEERIS
                     AGRADGEILVKEAERLRNKVPAGWVTVALDRTGRTLDSREFAEALRSWQDSSVRGVAF
                     VIGGAVGLDPAFARGCALRLSLGPMTFPHDLALVVLTEQIYRAATIVKGLPYHR"
     gene            complement(11216..12979)
                     /gene="recJ"
                     /locus_tag="PLT86_04375"
     CDS             complement(11216..12979)
                     /gene="recJ"
                     /locus_tag="PLT86_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT35885.1"
                     /translation="MSGSRDPTWVFPPPCAEEEVRSVATRWDVPLTVAEILLRRVNNP
                     DSPAARDDAIARFLNPSFRHLHDPYLYNGMHRAVERICRAVVNREQVSVYGDYDVDGT
                     TATALMVEALQTLGVPVEWRIPHRTADGYGLSDRGVELLRDTAGTLVIVLDCGVTAVE
                     QIATLTREGRDVIVVDHHEEGETLPNALAVLDAKCARCTYPFPGLSAVGVAFKLLQAV
                     CDALRVPPGRILIPGLDLVAVGTAADIVPVIDENRVLMRFGLRRLRKSPRPGLRALLQ
                     VAGLAGKELTTSSIVFGLAPRINAAGRMGSAETSVRLLLTQDEDEARSLARELNQLNR
                     DRQGRDQDVFDSAHRQAEEQVAQGARALVLADERWHPGIIGIVAARLVEEFYLPTVMI
                     TGSTPFARGSGRSIDGFDLHAALGACANTLIGYGGHIKAAGLSIDPQRIPEFRQCLQQ
                     VAAERLTDDLLSPRLYLDGEARLSDITPEYLKAVARLGPFGPDNMQPVLVSRHVRAIS
                     DPLIMKDAHLKVEVEQEGVTREIIGYGFKWAAPLFYDAANRSVSAVDIAYVPEENVWR
                     GKSKTQLRLKAVRRSDEHGRS"
     gene            complement(13051..14931)
                     /locus_tag="PLT86_04380"
     CDS             complement(13051..14931)
                     /locus_tag="PLT86_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35886.1"
                     /translation="MTRRILACAWWGAMAGAAAAAAPAFGTDFTLAFGKNLNVWSWNG
                     IFAHNRQLSSKWSAGTNASFDRKVSDSPGYLRRSTVVGGDVRTAYVVHPRLTLALTGK
                     ADLSRVQSGASISEVETNEILPTVLLTPVTGLSVKQGVGRAYDRRRNVSDAGLAYETG
                     VTFRPLQEPLGSRGWALVADYQHKGVSSRQGDVTKAFTLTTSYAREGWLNEDLRYSET
                     RDNQKYVSLLPANPILRRNVTGRSIRSSTMAVVPLLGSVQADAGYTRNSVEDDASNDP
                     NDLKYRTNNTTRGWMASANWTLPFSDPVLRSSISMGRTHRDAEPIRSAVDTTLFLRND
                     LDRRMSELALSVSAFFRVAAQDSFVVSGTLALNKDRTPAETELNDRDDYRRSLVGAYW
                     HTFGRSTVLELRGERTETHSVWLKTQRSSGNRWDRVLNLWAITNFERGRLGLSQRTFF
                     RTAMEEFDYDYLTPTNPQSRNTRLGRLEWQAKTRVGERDSVGISYSVEARTLGNLLPS
                     GPGQRPKLWQLTRNELIHVVSVSGVVRAGSSWKIAPSVAYDRQQGYVPTQAQWNPFAL
                     GRRVDKQEGFHAEAAISCAPPNGLFSRRDEITASVSRSYRKRAGLTDTMNYISVIYRL
                     HP"
     gene            complement(14928..15761)
                     /gene="rsmA"
                     /locus_tag="PLT86_04385"
     CDS             complement(14928..15761)
                     /gene="rsmA"
                     /locus_tag="PLT86_04385"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT35887.1"
                     /translation="MTGPGGTQWRPRKGLGQNFLVNEGIARRIVDAAGIAPHETVLEI
                     GPGMGALTRWLCEKAARVIAVELDPVLARELRLNGPASNLVVVEQDVLRTDLAALGRE
                     YGIDRWHIAGNLPYVIGSRLLMRLVEQARVVERATVMLQYEVAERILAPSGSRTYGLL
                     SVLLQATGRVRREFRVGAAAFRPAPKIQSLVFTWESTPAPHLDIPALIATTKAAFNQR
                     RKRLDNALKLLGVSADVIAEACRRAGIDPGNRAEQVSPSEFVRLSRACNELGVFPAEP
                     R"
     gene            complement(15761..16636)
                     /locus_tag="PLT86_04390"
     CDS             complement(15761..16636)
                     /locus_tag="PLT86_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35888.1"
                     /translation="MAKQTGIGCERCERFGRLGYRLDTDRGQDNPTGRVVACECAREL
                     CTCGALHEPAFAPAPPYEQLPERAAAAEDETACVCWRVRRHVTTLNRLLRDAGIPNLY
                     RGKLREDYHVRYPEGQVIPDAESARGQAVTWLETAVKEPTGKSLFLYGVPGDGKTLLA
                     SAFLTEIILLTRKSGLFVNLSRGYFQRLRNTYEDEPSIETTDQVFNRLTRVPFLVLDD
                     IGVERGSAWEIEWLYNLIDARYQNQNRLIVTSNNSLEELNVLSHGRISSRLRHACMVV
                     RLPDVDLRERFRYGA"
     gene            complement(16801..18489)
                     /locus_tag="PLT86_04395"
     CDS             complement(16801..18489)
                     /locus_tag="PLT86_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35889.1"
                     /translation="MIVHPGIPENLRWFDESRFGMFIHFGLYALLGRGEWVMYHENIS
                     RPEYRKLMTVFNPEKFSADEWVGLAKEAGARYITVTAKHHDGFCLFDSSLTDYKITNT
                     PFGRDLIGELVDACHRADMRIIFYYSQPDWYHPNFVHNRGAFKDLPHPPPTDKPDWEK
                     YVRYFHGQVRELCTKYGRIDGIWFDGSHKTVEEWRGKELYDMIKELQPNAVVNDRARW
                     GDFFTPERSLPDDLTGYMFEACESISPTAWGYQDDMPAHTVPHLVQSLVRMAAAGGNY
                     LLNVGPKPDGTIAEEQAAVMREIGKWLTKNGESIYRSHAGPRLEDPNIRVTRKGTTLY
                     LHLLEWPARNRIVLPNVIAEGVKEAALLGSGSELEVVETDEGAELRGLPIMPPDPLVN
                     VIRVEFFRLPEHFTFGAKEQAVAVPQPVSVSASSPTHLTPETARFEGLGVKGAKLRVR
                     RNEAGKAFISGWMVPDHAAHWDARVEESGRYRIDVVVAAPETHAGAVVKLIAGSTELT
                     FTAPETRSLDTVETVHAGTVELSAGVTRITLQPEKLKWGYLSPDIAEVVLTPVG"
     gene            18691..>19694
                     /locus_tag="PLT86_04400"
     CDS             18691..>19694
                     /locus_tag="PLT86_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOT35890.1"
                     /translation="MKAAVMRGIRDVRIEDIPVPPVLDEEALVRIRSVGVCGSDIHYY
                     THGKIGRYVVQPPFLLGHECAGEVVEVGAAVRNVRPGDLVAVEPGVPCRRCRFCREGR
                     YNLCPDVVFLATPPVDGAFAEYIAHSADFLFPLDGVSLHEGAMCEPLSTGMQAVRRSG
                     LRMGDTVFIAGVGPIGLCALQAFLAGGAAAVYVSDLDADRCRRAVALGATQAFDASDS
                     ELAAKLRDLTHGLGPDIVVETAGSEKATAQSVQMVRAGGVVVLIGLSPAAIIPIDTIA
                     IIDKEIDIRGVFRYANTYPAALSMIRRGAVDVKSMITAEYPLDRAKQALDDVAARKPG
                     V"
BASE COUNT         3635 a         6262 c         5856 g         3941 t
ORIGIN      
        1 gcatcgcatt ccgcagtttc gcggagttga catccgcgac tgcgccgttt tcccgtagcg
       61 ccagcgtcga ggccaccccc gctgcctccc ccgtctggtt gcagttgacc atgacacgca
      121 gcgcaccgta cgcgccgcgg tcgcaatcaa tcaggcgccc cgccaccagc acgtttttcg
      181 agccctcggg cacaagcgaa cggtacggga actggtagaa cgccggcgta tcctcgcctt
      241 caggtcgcca acgccctcgt accggcggtc ttcccggaac cacccattcc tccgtcccgt
      301 cgaggtttcg gaaggtgagc cccggttttt cgctgtggtg gacgtcaacg tgatacgtgc
      361 cgcacccgat cgtgtcttca aatgcatgcc cggtgaggag ttccatttcg gtcacgctgt
      421 gcaggcattt tgcgtggcgc gtttccctga taccgatgta cgacggcacc ccgcgaagga
      481 atacctcctc gccgcgcgca aaatggttcc ggaggatgtc caccatcgcg cggatttgcc
      541 gccttccttc catttcggcg gcggtgagct ggtctgcgtc ggagcaatcg gcattcgcaa
      601 cccgggtgcc tgcaaccatc ttcatgttgc gagatcccgc cacccacgct gcccagagga
      661 accccagctt gagcgcttcc gggaattccg gcttgaacac cgcctcgtta agtttgaacc
      721 ccggattcgc ttcttcaact ctgccaagcc cctcgataat gcaacacgcg gtgggtggct
      781 ggatatcgcc caggaaacgc gttggcagac ccatgcggtg gatcaggtcg ccgtcgccgc
      841 tcgtatccac gaagtacttc gcacggatcg ctcgacggcc tgacttgtcc tcgataatga
      901 cggcgtccaa cctgccgtcc gcaaccactg gagcgacaaa ccgggcatgg agaaacgggc
      961 ggacgttcgc ctcggtgatc aggttatcga gctcgatttt cagttcttcc gtattcagga
     1021 agtacgcagc gtggggagat gtgccgaatt ccaccgcggc gcggcggtgc agccggtcga
     1081 tcgtctcctg cgtaaggccg ccgatgatct ggcgctcgtc gtggacgtcc ttgagggagt
     1141 gccacacgtt caccagtccg gccgtcgcga cgccaccgaa aaatccaccg agttcgacga
     1201 cggcaactga ggcaccgagg cgggcagccc tgactgccgc gaataccccg gtgcaactgc
     1261 caccgacgac gcagatgtca gcctcgtgga cgatgggaac ggtgcgggaa ggttcagtga
     1321 tggtgagcat gtgttgctcc tcgcgcattt ctgttggcgg ctgtctccgc tagacagccc
     1381 tgccacgggt aaccacccta agaaatcggt cccatacgac acatacgtcc tatgccgaac
     1441 cgccaacagg gcatattgct tccggcgcta ctcccgcttt tccggcgggc gcgggcccac
     1501 gtccgacatg tcgatcggct cgaacccgag cgcgaaggcc ggggaagccg gttccaaggt
     1561 gaaatctcgc gccgcgaagt cccgacattt cggatccgcc acaattgact gtgggtcgta
     1621 cccgagtttc cgccattctt cgaatgaata ctcctgcttg tacccccact cgcctttctc
     1681 atttcgcgtg ctggcgccgc accacggggc gcgggcgaca tcccagaaca ggttgttgtt
     1741 ggaaaggaaa atacgctgtt cgggccttcc catggcgaaa atcggctggt tatccccgat
     1801 gaagatgttc cggtcgaacg tcaccgacag gtgatcctca taacgggaga ccgtcgcctg
     1861 cccttcgcgg ccgaaggcga agatgttgtt gcggacaacg ttttctcgcc cgtaatgctg
     1921 atggaacccc tggctggagg tattgtagca gatgttgttc tcactgatga tgtgcgagct
     1981 gccttcgtcc atgtaaatcg cccagccacc gtaattcgac ttttcgatgt cgtggataag
     2041 gttgccccgc agcatggtgc cgggctggac gccgagcgtg tagatgccgc ccatgtcgct
     2101 cagaataccg tggccgatgt cgtgaatgtg gttcttctcg atgcggttgt tctgtgacac
     2161 gttttcgtga tatccccaca cccaaccgca cgacacgccg ctgtagtaga ggtcgtagat
     2221 gtggttgtgg gagatatggt tgccgaatga atgccgggaa agcaccccga ccgctgcgag
     2281 aaacacgcgc ccgccttctc ctattacatt gtcggtgatg cggttgttac cggtccgccg
     2341 agcgcgtgga ccgagcgcat ccgctccgtt gagatggacg ccgccagcgc cgaggtcaac
     2401 cagggtgtta cccaccacgc ggatgttctg gcagccatcc cgcagttcga cggcatagcc
     2461 gccgatgtgt cgcaccgtgc agttctcgaa agcgcacgaa cgcgcggcct caaaggtgat
     2521 caccgccggt gttttatgag cggcctgatt cgacaggcag taatcccact ccgcatgctc
     2581 gaaggttagc ccttcgaagc gaagaaactc gacgtagttc tccgcgtcgg catccccggt
     2641 cacctgcacg agctttcgaa ggcgcggcgc gaacacctgt gcggtttcga tgtcctctcc
     2701 cggcatcggc acgtaggtca gtttcccggt ggacctgtcg aggtaccact cacccgggtc
     2761 gctcagcgct tccttcacgt tttcgacgta gtaccgggca agaccgtcgg cgacgtcgtc
     2821 tttcagcgtg aatcccgtct tgtttgcgag tttgaccgtg ttcgtggcgg ggtcgaaacc
     2881 tgccatggag actcgctctt ccacccacca gtggatcacc acgatgtcga tgtctgtgat
     2941 gttcttccag ctctggacat gtcccggcgt gcactggaag gcatctgctc ccatgaagat
     3001 atccccccat ggtccttcgg cgtgcggccg ttcatagtcg gcaacccggt agaagccctc
     3061 cttcggcaac ctcgggcggc tgcgtcttgc gccattcaca aacaactggc ggaaatacca
     3121 tttcccttcc gctacctccg ggatggtagt tacccagact tcctttccgt tcggcgattc
     3181 cttctgccat ccggtgatcc gcgtccctcc gtcaagcacg ggtgtttcgc ccgggaatgc
     3241 ggcgtaggtg acgggcgcgg aatcggcatg cgtgaaagtc agcggcgctt tgaggatgta
     3301 ccgccctccc cgcagccaga ccgttacgga tgcgggcagt ttggtggcac gtttcagctc
     3361 gcgaatcgtg tcgcgcgcct tggtcacggt tgcaaacggg ccgtcggtgt cgtcggcgtt
     3421 ggcttccgcg aggcgtccgg accagttatc gcgcccaagc ggggatacgt agagatcgag
     3481 atgggattgt ggcgcgggca tggaaactcc tcctgtatgc agatggatgc gttcgatcgc
     3541 gcgagcgcgg tgttacacga atctcatggc gaacaggtcg gcactgcgca tccggacgcg
     3601 gaaacgaact tcggcgtttt ccgcatgccc gatgtcgctt tcgccgcgcc acgtgatggg
     3661 atggtccgtt tcatccccgt tgaagcggtc gcaatcggcg aagctgcgcc cgggaattgg
     3721 tgaaccgtcg ggtttgcatg cttccacctc aacgaaaccg gtgaattgcg ttttcgcgtt
     3781 cagccgcagc gtgcgtccct caaaaacaag cggttgtgta gcgaagaaac cctccatcgg
     3841 gcattccagc gcaacaagac gctctttttt ccatgtcgcc cacgcgattt tgcccagagg
     3901 cggctttcgc gggtgcttgt gcggcaccgg tgagcccgcg aacagcatcc cggtgcggtt
     3961 cccgggaagg tccacgaggc cgattccggg ggtgacgacg cccccgtccc actcgcccgc
     4021 gcaccccggt gagcagaccc cctcgccggg cgtgtagccg ctcgcgtccg gaacgaaacc
     4081 ccagacgacg ccatcggggc tcgtggcgag atggaaatgg aacgaatccg tggcaagcga
     4141 ccaccgcatg gggaacatca cgtggtagtc gatggtgccc ggcatcagcg ttttgggctg
     4201 gctgtaccag agatcgtggg gagccatcgt ggcaccgggc cagaagaggt cctctggaag
     4261 agggaaacgt cgaaaatcgt ccgtttccat ccgcccgatc gtgcggcggc ggaagaacca
     4321 gttgcggcag taggcgacgt atttcccctg ctgaacgtcg tacgtgcagc agttgtgcgt
     4381 atcgctgtag tgggccacga gcggctctcg gatcggtttc cagtggatgc cgtccggcga
     4441 cacggcacca aaaaggccct ggtttctgtg gtacgggtcc acggcatccg gccgttcgcg
     4501 gaggtagtcc gcatacatgt catccgggat gtgccccaga tggaacccct tgtaccgttc
     4561 ttccggagga gcggacgggt ctttgaatac gctcccgccg tggtacccca cgtcgtcatc
     4621 cctgagcatg gcaccgtaga cgatattgtt gcgggtgtct ccccgataat tggccactcc
     4681 gacgtcggga aagcgccagt ccacgccgtt gtcgctttcg gcatagcgga cgtggttgcg
     4741 gacacccatg tgcggtgtgc caatgccctc ttcgggccag cactcgtacc agaaccggta
     4801 gacgccttcg tcatggatca gtgtgccacc gaacaagaac actgcacgtg ccatttcggg
     4861 cgtgatgacg ggttggcctt tttctgcctt ctgcgccaca aggcggatgc catacggcat
     4921 gtctacgcaa cggtaacgga ggggaggcaa gctctccgtg gtgaaaagcg gcacgccttt
     4981 accggtttcg gtgagccact gatgggagcc gtgatccacg tagcgccaat cgtggaagta
     5041 gataacgttg gacgcgaggc gaaacggggg acggttgctg tggattacgg acttcacgag
     5101 ggcgttctcc ttcggtagca acccatttcc ccgttgctga ccggttgttt cttacacgga
     5161 acggttcgcg actgctttcc gtatcacttc gtcaagctgc gcgtagttga ggtcgtcttt
     5221 ctgcgcgaac ggcaaatcgt ccgcctgcgc cccgcgatta agaaacgcgg ataggccgat
     5281 cgccttcttt ccgatgaacg cgctggcccc ccagcgttcc gccatggaac ggaatttgtc
     5341 cgcgtcgaaa ttggtaagca tgactaccgg aatgtcgcgc gtcttctcgt cggcaagaag
     5401 gaacttcaga gtctcatacc catcctgccg cggcatgacg aggtccagca ggaccacatc
     5461 cggttgttcc gctttgatgc gttcaaccgc cttcactgcg tcggtctccg aggcaaccca
     5521 gtgcccgaac ttcgcggaga gcacggcggt gacaagttga acgaaggcct cgtcgtcgtc
     5581 cacgacaaac acgcggagcg gttccatgtt accccccact tctgcggctg tgaccggccc
     5641 tgcaaaacga gccgtcgccg cctcatgcat acccgtcgtg cgattgcgtt cgcccgttac
     5701 gaggaacaat tccggcatct gcaataagaa cgcaaccgcc ggcgcttgct ccaaatccag
     5761 ccattccttt cgttgcggaa gcggagcacc gatttgcggg attggcaccg ctgtcaggtt
     5821 tccacgaatc gtgcaacagc aggaagtatc tcccgccgga tgctgtccac attccagatt
     5881 acgatcacgc gcgggcttca gtccggccgc acgtccgcgt cctcttcgcg cgttcccgaa
     5941 taccggacgt cctcgccttc tccgggctgc tccacgacgg gaaccgtgaa cgtaaacgtt
     6001 gccccctgcc ccggggtgct ttccgcccag acgaagcctc cgtgcgcttc cacgaggaac
     6061 ttgacgatcg taaggccgag gccggtgcct ttcgccttcg tagcgcgttt catacgaacc
     6121 tggtggaatt tgtcgaaaat ccgcggcaag tcttccgggg gaatacccac gccggtatcg
     6181 cgcacggagc agcgcaggaa cgcgccctcc tgctcgcagc tcaacgcgat ctctccgttt
     6241 tccggtgtga acttgaccgc gttgccgagc aggttcatga gcacctgctc catacggttc
     6301 ggatcagcat acgccggcgg gaggtccggt gcggccttca gggtgatccg tagccgtttc
     6361 tccatggaaa gaggcgcgat agacgttgca acccggcgaa tgatggcgac gaggtcggtc
     6421 gtttccggtt gaatttccag cttgcccgcc tcaagcctgg ccgtatcgag caggtcgttg
     6481 atgaggttcc cgagacgttt cgcgttcgta cgaatgatct gcaggatttc tttctggtcc
     6541 tccttgagcg ggctcgggat gccatcgttg agcagttccg cagcctgctt caccgcggcg
     6601 atgggcgttc gcagatcgtg ggagaccttg gagaggaaat ccgccttcaa cgcatcaagt
     6661 tcaccgagac gctctaccgt ggcattcaga ccctcggcga tttcggaaaa ttcgtctttg
     6721 cttaccagtt ccacgcgctg ctgaaaatcg ccgctggcaa cagcgctcaa tgcgttgctg
     6781 atgccggcgg tcgcccgctt gatcgtccgg ttcagataca tcaccatggc gagcattccc
     6841 gccaggagaa ccgccagaac cacgcccgcg cgcaggatca gatgctccct gaaccgcgac
     6901 gccgccgcga ggaggacgtt cgcgtcctcc tgcgcgtgtt cctgcagact cgtcatcgcc
     6961 cgggaaagct cggcgtccac ttcgtgcaac tggctgaatg cacgctcggc ccggcccggc
     7021 tgtccgcgtt cgaagtgcaa ttccacgctt tcaaaaagca gatcgagctt ccggcacgcg
     7081 atgagaaggc tgtcaaggct gtacttcagc gcggggcgct gcccgaaccg ggaccggctt
     7141 acctccgcca gcagggaatc acggtgcgca cgccgagagc gaagcgatct cgtcatctca
     7201 tccgcgtcgc gcgatgcggc gaacgcgccc acctggaaca tcgagacttt gattccgccg
     7261 cggtacagga tctcgttgat gtcatcggag atcctgttga tccggatgat ttgttcctgc
     7321 gcattcacgg tgagttctac caggtcgtcc atcctccgca aatcacgaac gacccagagc
     7381 accgcggcac acatcagcgc aaccataccg aggttgccaa ggagaacctt tccgccgatg
     7441 gtgagccgca cgctgtctcc tcctcaactg gtcatcgttg cgcggctgta ccgcgaaccc
     7501 gcgtgcgcaa ccgcaaggcc acgcatctcc atcagcgtga gactcgtcag aagttcctcc
     7561 gatttcctgc ccagtttcac cgccaatgca tcaacgtgca ccggttccag ggccagcgcg
     7621 tcgagaacca cccgctcctc gggggagaga tccgccggag ccgataccgg gagaccaagt
     7681 tgatccccct ccggcgtggg aacacgggta ccgagcccca gactgattac cagatcatcc
     7741 atgtcacgga cgacacgcgc cccatccgcg atcaattcgt ttgcacctgc cgcttcccgc
     7801 cgcgtgatat ctccgggaac ggcgtacaca tcacgcccct gcctgagcgc gacatcggcc
     7861 gtgatgagcg cgcctgaggc ccgtggcgcc tcggtaacga tcaccgcacg cgaaagcccg
     7921 ctgatgatcc ggttgcggcg aggaaagttg attcggtccg gcgccgcacc gagccagact
     7981 tcactgacca gaagaccgtg ttcgcgaatg cgtgcatgga ggttccgcgc ttcaggtggg
     8041 tataccacat ctaccccgca tcccagcgcg gcaaccgtca atccgtctct atcgagagcc
     8101 accgtgtggg caatactatc caccccgcgc gccatcccgc tgatcaccgt caccccgtga
     8161 cgaacaagcg tcgccgcaat cgcttcggtg atatggcggc cgtactccga tggccgccgc
     8221 gtgccgacta ctgctacgcc cggaatcgcg aggagccccg tgtctccttg cgcaaagagg
     8281 agttggggcg ggtcctcggt ttccagaagg tgaggcgggt agtctgcgtc gatgcacgcg
     8341 atgacggaag tgtttgtgcg gacgatgcgc tcgcactggc gcatgactgc atcgcgcacc
     8401 gtatcagtcc tgccgcgacc catgtcagcg attgccagag cgattgcgga agggataccc
     8461 tggaccgttt ccagctccga agggttggct gccaggactg caccaggcga accgaatgct
     8521 tccacgagag cggccagacg cgcggcgccg atacctttga cgttacacag cgcgagcagg
     8581 tcgcccagtt cttcgaagga acggtcggac atggcttatt gaggcggact ttcggggagc
     8641 cgcgtggcga aacgcacccg tgaatcggaa tcagttcccg cgtccgtcgt ttcggtaaga
     8701 agaacagaga gctcgtgccg cagtcggtca aggccgacat gagtgacggc actcagagag
     8761 aaacaggcgt gcgcgccacg agcttttcgc ggctttgcat cgctgaggtc tgctttgctc
     8821 aacacgtata cccacggctt tttcaacagg tcggggttga actcgcgcag ctcgttgcga
     8881 agaagtttca gcgtcgcgtc cggatcggga tcgatgctct ccaccatgaa cacaagcacc
     8941 cgggtgcgct cgatatgctt caggaactgg tcacccaggc ccttacccag atgcgcacct
     9001 tcgatgagac cggggatatc cgccatcaca caggtcaggt gctccgccag cgacacaatt
     9061 ccgaggttcg ggacaagcgt ggtgaaagga tagtccgcga tttttggccg ggcagccgac
     9121 atgaccgaca ggagggttga tttcccggca ttcggcagac ccacaagacc tacatcggcc
     9181 agcaatttga gctcaagccg aagagaacga tactctcccg gcgttccgtt ctgggcgaaa
     9241 tcaggcgctt gccgggtcgg tgtggcgaac tgggcattcc ctttcccccc acgccctcct
     9301 ttagccacga tcaccggttc gtgtggggcg acgatgtcct gaatcaatgc gcccgtttcg
     9361 gcgtcaaata caagcgtccc gcacggcacg cgaatagtca catcctctcc tgatgcgccg
     9421 tgccagcgtg atccgagccc tcgttctccg tgcctggccc ggaaatgccg gcggaagcgg
     9481 aactcctgca acgtattcat ccgcggatct ccgacaaaga ggacattgcc tccgttgccg
     9541 ccgtctccgc catccggtcc gccgcggggt acgttcttct cgtgtcggaa actgacggct
     9601 ccgggcccgc cgtctccgcc ccatacctcg atagttgccg tatctacgaa ttccatgttc
     9661 ccgcctccgg cagttgtccg agcgactgcc gcgccccggc tttcaattaa caaacctatg
     9721 gaatgggcgg accttttcat agacccggca ccggggaaaa agccctcaag tccacgcacc
     9781 tgcgcacgcg tcgcacccga aactgccacg gccggtgtcc cgggtcttga aatcacgccg
     9841 gtgttttctg tatacttatt agttctttga acactgtggc ccgttcgtct agtggttagg
     9901 actcagggtt ttcatccctg caacaggggt tcgactcccc tacgggtcac caggtgcttt
     9961 cagagaccgg gtaaggagtc tgtccgcgtc ggggaacgca ttcagggtcc gtgcccggca
    10021 cttccctctg aggtgaaaca cgatggcaac gcagcctttt tcgctggacg ccattcggtc
    10081 cgtccctctc tttaccaatc tttccgacga aatgctcgaa gccgtggtga aggcttccac
    10141 ggtggtaaca ttccggcgga accagaccat tgtccgagag aatgaggaag ggaacgcgct
    10201 tttcatcatt ttgagcggca gggtgaaagt cacgctgatc cgctcagatg gcaaagaagc
    10261 gattctggcg attctgcgcg cgaatgattt cttcggcgaa atggcactcc tggacgatca
    10321 accgaggtcg gcatccgtgg tggcgctcga aagcacccgc gcgctcatgc ttacgaggcc
    10381 gaatttcatc aaattgcttg aggatgaccc gcagattgtc cacaacatca tggttgcgct
    10441 gagcgggcgc cttcgcaagg caaaccagaa aatcgccgac ctcgcattcc tggacgcgat
    10501 cggccgcatc tgcggggtaa tcctgcagat ggcggaggaa gcgggcgaag aaaccgatga
    10561 aggtatcctg atccataacc gcccctccca cgagcaactc ggaatgatgg ccggcaccac
    10621 gcgggaaact gccacacact gcatgattgc gcttgaacgg cgggggtacc ttctgacaca
    10681 gggtcgcgac ctgctgttgt tttcgccgga ggacctcaag cgcgattttc tttcccccag
    10741 ttcgtaacgt gaagctccgt attgtggtcg tgggcaaacc gcgacgtgat gtcgtcgaag
    10801 cgttcgttgc cgactatctc gcccgtttga aaccgatctg gaagacggaa ctcgtcgcgg
    10861 tggctgagga acggatctca gcaggccgtg ccgacgggga aatcctcgta aaagaagcgg
    10921 aacgactacg gaacaaggtc cccgcgggct gggttacggt tgcattagat cgtaccggca
    10981 ggacgctgga ctcacgggag ttcgcagagg cgctgcgctc gtggcaggat tcttcggtgc
    11041 gcggcgtggc gttcgtgatc ggtggtgcag tggggctgga cccggcattt gcacgcggat
    11101 gcgcgctccg cctatcgctt gggccgatga ccttcccgca cgacctcgcg ctggtggttc
    11161 tgaccgagca gatttaccgc gcggcgacga ttgtcaaggg acttccctac catcgctaac
    11221 tgcggccatg ctcgtcgctc cttcggacgg ctttcaggcg gagctgcgtt ttcgacttcc
    11281 cgcgccacac attttcttcc ggcacatagg cgatgtcaac cgcgcttact gaacggttcg
    11341 cggcatcgta gaagagcggc gcagcccatt tgaaaccgta gccgattatc tccctcgtta
    11401 caccctcctg ctcgacttcc accttcagat gggcatcctt catgatgagc gggtcggata
    11461 tcgcgcgtac atggcgcgaa accagcaccg gctgcatgtt gtccggcccg aaaggcccga
    11521 gacgggccac cgctttcagg tattccggcg tgatgtcgga cagccgtgct tcaccgtcga
    11581 ggtacagacg cggggagaga aggtcgtccg tcaggcgttc cgccgccacc tgctgcagac
    11641 actgtcggaa ctctggaata cgctgagggt caatactgag gcccgccgct ttgatgtgcc
    11701 ccccgtagcc gatcagcgta ttcgcgcacg cgccaagggc ggcgtgaaga tcaaacccgt
    11761 ctatgctcct gccggaaccc cgcgcgaagg gtgtggagcc ggtaatcatg acggtaggaa
    11821 ggtaaaactc ttccacgaga cgtgcggcca caattccgat gatacccggg tgccagcgct
    11881 cgtccgcaag caccagcgct ctggcaccct gcgcaacctg ctcttccgcc tgccggtgtg
    11941 cgctgtcgaa cacgtcctga tcgcgcccct gccggtcccg gttcaactgg ttcagttcac
    12001 gcgcgaggct tcttgcctcg tcctcatcct gcgtaagaag cagtcgaacg gacgtttccg
    12061 cgctgcccat ccgtccggcc gcattgattc tcggcgcaag accgaatacg atgctggacg
    12121 tggtgagttc tttccccgcc agcccggcaa cctgcagcaa cgcccgcagc cccgggcggg
    12181 gtgatttcct cagcctgcgg agcccgaatc tcatcagcac gcggttctca tctatcacag
    12241 gcacaatgtc ggccgcggtt cctacggcca ccagatcgag ccctggaatc agaatcctgc
    12301 cggggggaac gcgcaacgcg tcgcacacag cctgaagcag tttgaacgcc acgccaactg
    12361 cggacaaccc aggaaagggg taggtacagc gcgcgcattt cgcgtcaaga acggccagtg
    12421 cgttcgggag cgtctcgccc tcttcgtggt ggtccacgac gatgacgtcc ctaccctccc
    12481 gcgtgagtgt ggcgatctgc tccaccgccg tcacgccgca atcgagtacg atcaccagcg
    12541 tgcccgcggt atccctgagc aactccactc cgcgatcact caggccgtag ccatctgcgg
    12601 ttcggtgcgg gatgcgccac tcaaccggaa caccgagggt ctggagcgct tcgaccatga
    12661 gcgcggtggc tgtcgtgcca tccacgtcgt agtcgccgta cacgcttacc tgttcccggt
    12721 tcaccaccgc acggcagatc cgctcgaccg cccggtgcat cccgttgtac aggtacggat
    12781 cgtggagatg tcggaagctc gggttcagga agcgtgcgat tgcgtcgtct ctggcggcgg
    12841 gggaatctgg attatttacc cggcgcagca ggatctcggc aaccgtgagc gggacgtccc
    12901 accgcgttgc aacactgcgt acttcctctt ccgcgcacgg aggaggaaaa acccacgtgg
    12961 gatcgcgtga tccgctcatc agtacatgct ccggggtgcg ctactgcgtt aaaaaccgca
    13021 ccgtgaaacc ttcggatgcc gccggtgtgt tcagggatgg aggcgataga taacagaaat
    13081 atagttcatt gtatcggtca gcccagcacg cttccgatag gatcgggaaa cactggcggt
    13141 aatctcgtcc cgccttgaga acaacccgtt cggcggcgca cacgagatcg cagcctccgc
    13201 gtggaacccc tcctgcttgt ccacgcgcct gccgagggca aacgggttcc attgcgcctg
    13261 agtcgggacg tatccctgtt ggcggtcgta cgcaacggag ggtgcgatct tccatgacga
    13321 cccggcccga acaaccccgg acacgctgac aacgtggatc agttcgttcc gtgtgagctg
    13381 ccatagtttt gggcgctggc cgggtccgga gggcagcagg tttcccagcg tccgcgcttc
    13441 caccgaatac gaaatcccga ccgagtcgcg ctcaccgact cgggttttcg cctgccactc
    13501 cagccgtcca agcctggtgt tccggctttg cggattcgtg ggggtcaggt aatcatagtc
    13561 gaattcttcc atcgcggtcc ggaagaaggt gcgttgcgag aggccgagcc gccctctttc
    13621 gaaattcgtg atggcccaca ggttgagcac ccggtcccac cggttcccgg aagaacgctg
    13681 tgttttcagc cacacgctgt gggtttcggt tcgttcgccg cgcagctcga gtaccgtact
    13741 tcgcccgaat gtatgccagt aggcgccgac gagagaccgg cggtaatcgt cccgatcgtt
    13801 caattccgtt tccgccgggg tacggtcctt gttcagcgcc agcgtgccgc tgaccacgaa
    13861 tgaatcctgg gcggcaacgc gaaagaacgc cgacaccgag agagcaagtt ctgacatccg
    13921 gcgatcaagg tcattgcgca gaaaaagcgt cgtgtcgaca gcggagcgga tcggctccgc
    13981 atcacggtgc gtccttccca tggatattga ggagcgcagg acgggatcag agaaagggag
    14041 cgtccagttg gctgaggcca tccagccgcg ggttgtgttg ttggtgcgat acttcaaatc
    14101 gttcgggtcg ttgctcgcgt cgtcctccac ggaattccgg gtgtacccgg catccgcctg
    14161 aacggaaccc agtaacggaa ccactgccat cgttgaagac cggatgctcc ggcccgtaac
    14221 gttcctgcgc aggatcgggt tggcaggaag gagacttacg tacttctggt tgtcgcgcgt
    14281 ctcgctgtac cgaaggtcct cgttgagcca gccttctctg gcgtacgacg tggtgagcgt
    14341 gaacgccttt gtaacgtcgc cctggcggga agagactccc ttatgctggt aatcggcgac
    14401 aagcgcccac ccgcggctgc cgagcggttc ctggagcggg cggaacgtga ctccggtttc
    14461 gtatgccagc ccggcgtcgg agacgttgcg gcggcggtca tacgcgcgtc ccaccccctg
    14521 ttttaccgaa aggccggtaa ccggtgtgag aaggacggtg ggcaggattt cgttggtttc
    14581 gacttcggag atgctcgcgc cgctttggac gcgcgacaga tcggctttcc cggtcagtgc
    14641 cagagtaagc ctcgggtgga ccacatacgc cgtacggaca tccccgccca ccaccgttga
    14701 tcgcctgagg taacccggcg aatccgacac tttcctgtcg aacgaagcgt tggtgccggc
    14761 gctccatttc gaggaaagct gccggttatg ggcgaaaatg ccgttccacg accacacgtt
    14821 gaggtttttg ccgaacgcca gtgtgaaatc cgttccgaat gcgggcgcgg cagctgccgc
    14881 cgcgcccgcc atggcccccc accacgcaca tgcgagaatg cgccttgtca tcgtggttcc
    14941 gccggaaaca caccaagctc gttacacgcg cggctgaggc gaacgaattc tgacggggac
    15001 acctgttccg cgcggttgcc cggatcgatg ccggcacgcc gacaggcttc cgcgatgacg
    15061 tccgcggaaa cacccagcag tttcagcgcg ttgtcaagcc gttttcgcct ctggttgaat
    15121 gccgcctttg tagtcgcgat aagcgcggga atgtcgaggt gtggcgccgg agtgctttcc
    15181 caggtgaaaa cgagcgactg gattttgggt gcgggtcgaa atgccgctgc gccgacgcga
    15241 aactcccgcc ggacgcgtcc cgttgcctgc agaagcacgc tcagaaggcc ataggtgcgc
    15301 gacccggaag gcgcaaggat ccgttccgcc acctcatact gcagcatgac ggttgcccgc
    15361 tccacaacgc gcgcctgttc caccagcctc atcaacagcc ggctgccgat gacgtacgga
    15421 aggttgccgg caatgtgcca ccggtcaatg ccatactcac ggccaagcgc cgcgagatca
    15481 gtgcgaagga catcctgctc cacaacgacg aggttcgaag ccgggccatt caagcgcagc
    15541 tcccgggcca gaaccgggtc gagttccacc gctatcacac gcgcggcttt ttcgcacagc
    15601 catcgtgtga gcgcacccat tccggggcct atctccagta cggtttcgtg cggcgctatc
    15661 cccgccgcat caacgatgcg gcgggcaatc ccctcgttca caaggaaatt ctgcccaagc
    15721 cccttcctcg ggcgccattg tgttccgccg gggcccgtca ttacgcaccg taccggaacc
    15781 gttcgcgaag gtcgacgtca ggtagcctca ccaccatgca cgcatggcgc agacgggagg
    15841 aaatccgccc gtgggagagc acgttgagct cctcaagcga gttgttgctc gtcacgatca
    15901 gacggttctg attctgatac cgcgcgtcta tcaggttgta cagccattcg atttcccacg
    15961 cactcccccg ttctacgcca atatcgtcca gaaccaggaa cggaacccgg gtgagtcggt
    16021 tgaacacctg atccgtcgtt tcaattgagg gctcgtcttc gtacgtattg cgcaatcgct
    16081 ggaaatatcc gcgggagaga ttcacaaaaa gccctgattt gcgcgtaagc aggatgatct
    16141 ccgtcaggaa cgcgctggcc agcagcgttt tcccatcgcc cggcaccccg tacagaaaca
    16201 gacttttccc cgtcggctcc ttcaccgcgg tttcgagcca cgtcaccgcc tgcccccgcg
    16261 cgctttcggc gtccggaatc acctgaccct ccggatagcg cacgtggtag tcttcgcgga
    16321 gcttgccgcg gtacaggttg gggataccgg catcgcgcag gagccggttc agtgttgtca
    16381 cgtgccgccg gacgcgccag cagacgcagg cagtctcgtc ttccgccgcc gcagcccgct
    16441 ccggaagctg ctcgtacgga ggcgccggag caaacgccgg ctcatggagc gcgccgcacg
    16501 tgcaaagctc ccgcgcacat tcacaggcaa ccacccgccc ggtcgggtta tcctgtccac
    16561 gatccgtatc caggcggtac ccaagccgcc cgaatcgttc acaccgttcg catccgatgc
    16621 ctgtctgctt cgccattatc ccctgctcct cattgatccc tgcggtcaga ctgtaccctt
    16681 tctgaaaaat gtctgttttg aggggacagg ggaaacccgc caaggctatg cctcattcac
    16741 agtcggcgcg gcggacgctt ctcttcgccg cttgagccgt ctctgccccg gtccgcgcag
    16801 tcaccccacc ggcgtcagca ccacctccgc gatgtcggga gagagatacc cccatttgag
    16861 tttctccggc tgcagggtga tgcgggtcac cccggcggac aattccaccg tgcccgcgtg
    16921 cacggtttcc acggtgtcca gactgcgcgt ctccggcgcg gtgaacgtca gctccgttga
    16981 tccggcaatc agcttcacca ccgcccctgc atgtgtttcc ggcgcggcaa caacgacgtc
    17041 aatacgataa cgccctgatt cctcgacacg tgcgtcccag tgtgccgcat ggtcgggcac
    17101 catccagccg gagatgaatg cctttccggc ttcgttcctg cgcactcgca gtttcgctcc
    17161 tttcaccccg aggccttcga acctcgccgt ttccggtgta agatgcgtcg gcgatgacgc
    17221 cgacacggaa accggttgtg gcacagcgac agcctgttcc ttcgcgccaa acgtgaagtg
    17281 ctccggcaga cggaagaact ccacgcggat cacgttcacc agcgggtccg gcggcatgat
    17341 cggcagcccc cgcagctccg cgccttcgtc ggtttcgacc acctccagct ccgaaccgct
    17401 tccaaggagc gccgcctcct tcactccctc cgcgatcacg ttgggcagga caatcctgtt
    17461 ccgcgccggc cattcaagca ggtgaagata gagcgtcgtc cctttgcgag tcacccgaat
    17521 attcgggtcc tccagccttg gcccggcgtg actgcggtag atgctctctc cgttttttgt
    17581 gagccacttg ccgatctcac gcatcaccgc ggcctgttcc tcggcgatag tgccgtccgg
    17641 tttcggtccg acattgagca ggtaattgcc gcccgccgcc gccatacgga cgaggctctg
    17701 cacaagatgc ggcaccgtgt gggccggcat gtcgtcctga tacccccacg ccgtcgggct
    17761 gatgctctcg cacgcttcga acatgtagcc ggtgaggtcg tccggcaggc ttcgctccgg
    17821 agtgaaaaag tccccccagc gcgcgcggtc gttcaccacg gcgttcggct gcagctcctt
    17881 gatcatgtcg taaagctctt tgccgcgcca ctcctccacc gtcttgtgcg aaccgtcgaa
    17941 ccagataccg tcaatccggc cgtatttcgt gcagagttcc cgcacctgcc cgtggaaata
    18001 gcgcacgtac ttttcccagt caggtttgtc ggtggggggc gggtgcggca aatccttgaa
    18061 ggcaccgcgg ttgtgcacga aattggggtg gtaccagtcg ggctgtgagt agtagaaaat
    18121 gatccgcata tcggcgcggt ggcaggcatc caccagttcg ccgatgagat cgcgtccgaa
    18181 cggcgtgttg gtgatcttgt aatccgtcag cgatgagtcg aacaggcaga atccatcgtg
    18241 atgcttcgcc gtcacggtga tgtagcgcgc gcccgcctct tttgccaggc cgacccattc
    18301 gtccgcggag aacttctcgg ggttgaacac ggtcatcagc ttccgatatt ccgggcggga
    18361 gatgttttcg tgatacatca cccattcgcc gcggcccagc agcgcgtaca gcccgaaatg
    18421 gatgaacatg ccgaaacggc tttcgtcaaa ccagcgcaga ttttcgggaa taccgggatg
    18481 cacgatcatg tgcgacctcc tcgaggtggt tgtttcgttt ctggcgtcct gtgaatacta
    18541 ccgcgcggaa cctgcaaatg gtttctgccc ccggaaccta ccggatacgt cgctctcttc
    18601 atgccggcgc caggacggat tacattctgt tgacaataac cggaagcagt cctttcccgc
    18661 aaaggaggcg ccagcctcca gggaggcatc atgaaagctg cggtcatgcg gggaatccgc
    18721 gatgtgcgca tcgaagacat tccggtgccc cccgtcctcg acgaggaggc gctggtccgc
    18781 atacggtcgg ttggcgtgtg cgggtcggac atccattatt acacgcacgg aaaaatcggc
    18841 cggtacgttg tccagccccc gttcctgctg gggcacgagt gcgccggcga ggtagtggag
    18901 gtgggcgccg cggtgcggaa cgtgaggccg ggagatctgg tggccgtgga acccggggtg
    18961 ccgtgccgca ggtgccggtt ctgcagagag ggccggtaca acctctgccc ggacgtggtg
    19021 ttcctggcca cgccacccgt ggacggggcc tttgccgagt acatcgccca ttctgccgac
    19081 ttcctgtttc cactcgatgg cgtgagcctg cacgagggcg ccatgtgtga gccgctctcc
    19141 acagggatgc aggccgtgag gaggtcgggg ctccgcatgg gggacaccgt atttatcgca
    19201 ggagtcgggc caattggcct ctgcgcgctg caggcgttcc tcgcgggagg cgcagcagca
    19261 gtgtacgtca gtgatctcga tgccgaccgg tgccgccgtg ccgttgcgct cggggcgact
    19321 caggcattcg acgccagcga ctcagaactt gcagcgaagc tgcgagatct tacgcacggg
    19381 ctcgggccgg acatcgtcgt ggaaacggcc ggctcggaaa aggcaaccgc acagagcgtg
    19441 cagatggtgc gcgcaggggg agtagtggtg ctgatcggcc tttccccggc cgctatcatc
    19501 ccgatcgaca ccatcgccat catcgacaag gaaatagaca tccggggcgt gttccgctac
    19561 gccaacacct accccgcggc gctctccatg atccgccgcg gcgcggtgga tgtgaagagc
    19621 atgatcaccg cggaataccc cctcgaccgc gcgaagcagg ccctcgatga tgttgccgcc
    19681 cgcaagccgg gggt
//
LOCUS       DAOSOO010000037        19610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2776_length_19610_cov_6.761749, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000037 DAOSOO010000000
VERSION     DAOSOO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2776_length_19610_cov_6.761749"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLT86_04405"
     CDS             complement(<1..200)
                     /locus_tag="PLT86_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35891.1"
                     /translation="MPALLPPKRTRIEAAATGVCPSPPVIPAQAGIQATLPGALPVHH
                     HRFDDPRLAGGNIIEGLLRAVEG"
     gene            complement(816..2009)
                     /locus_tag="PLT86_04410"
     CDS             complement(816..2009)
                     /locus_tag="PLT86_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT35892.1"
                     /translation="MSADAIPDHMKWWREATFGMFIHWGVYAVPARNEWVMYVENIPK
                     EEYAHFADGFTARHYHAREWVKLAQDAGMKYMVLTTRHHDGFCLFDSKVSDFTAPKTA
                     AKRDLIAEYADACHALGMRMGFYYSLEDWRFPHQLPHLPMREDKSVYQPMVEQAHAQV
                     RELMTNYGTVDILWYDGGFPAGIWRSEELNAMVRSLQPNIVINDRSGLPEDFGTPEQH
                     IAPQDRPWEACMTMNDTWGYTAGDKNYKSVRQILGMLIQCASQGGNLLLNVGPDAEGR
                     IPEPAVERLRVVGEWMRTNGKAIYGATRAPLVTHSFATPTRVGDRLYLLSAHWPGSTA
                     SFGWCGNRVLSAKLLATGERLIVEQKGDRVWLRGLPQYAPDVNVSVIEITVEGEPKWP
                     EVRFT"
     assembly_gap    2112..2211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2212..3632)
                     /locus_tag="PLT86_04415"
     CDS             complement(<2212..3632)
                     /locus_tag="PLT86_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35893.1"
                     /translation="MSPLLGMYVHMHWGYAYPYAARTWSLEDWRAYASGLRALGYNLV
                     MIWPLTETMPDPPTASDVAHLEKLGNVITMLQHEFGMTVLITFGANTIGNTHAAEYPF
                     ETRPFFRTDLRLNPADPSAMDRLMRFRASLYRYLAHADGIVVIDSDPGGYAGSTNAEF
                     TAILRRHLRLLRDANPKAMLWYWLWVGWESYNRFWEQAEKGGDAKLEVNPSDWAEVVE
                     DLCTTPDDPWRLLVCWDSHRRLAERLGVEERCVYFPYGAVEGEPTVPLTNCDPTGVEK
                     ALSGYPWGRPYLGCMANAQTHIVQLPNTYLFAHFARGGRLEDVDLAGFAEKLIPGQGS
                     LIAEAWKALADRAIEPARRLAKELTRGEARQLPRGEFSGLLMHDPTRFLGDLVLQLAF
                     SADLSDFAAAVDAGGDGTPEVRGLVASWREWTERTGFVDAYCGPVMDRLHPALRKLRS
                     PAVDAVLADFDNWRNPAVRNGIIP"
     gene            complement(3683..5677)
                     /locus_tag="PLT86_04420"
     CDS             complement(3683..5677)
                     /locus_tag="PLT86_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT35894.1"
                     /translation="MHATLLSDILIIFALSVAVVFICERLHVPSLVGFFLTGIVAGPH
                     ALGLVSDVARVESISEVGIVALLFTVGLSFSLEHALRVRRTVLVGGTIQIVATGAAGY
                     LAARALGQPPPVAVLAGFLLSLSSTAIAIKSLQDRAEADSLHGRIVIGILVFQDIITV
                     PMLFSLPLLAEATAQSAGSLLTMAAQGLGVIAIVIVSARWVVPYVLYQIARTRSGELF
                     LFAVVVICLAITWLTYSIGLAPALGAFLAGIIISESEYSQQALGDVIPFRVIFSNLFF
                     VSVGMLLDIGFLFDHAIAVAAMACGSIIIKVLLAGAATALLGYSLRTTIGVALGLGQI
                     GEFSFILAASGLQFGLLEPSGYQYFLTVSVITMAATPFLIQAAPHIADQLLRLPLPKR
                     VRAGSFAREDDGNAGLTDHLIIIGYGVNGRNVASAAAEALIPYVIIEMNPETVRRERA
                     NGESILFGDGSQPVVLEHAGILQARVLVVATPDATGTRRIVENARRMNPSVYIIARTR
                     FVTEVAPLYKLGADEVIPEEFETSVEIFSRVLRKYLIPREQIENFIRTIRAGGYDMFR
                     SFSGDFTALSEIQTSLRDVEVSTFPVDEGCRMAGQTLAELGLRSRTGITILGIRRGDK
                     TIPNPGGEETIHAGDVLLVMGPPDAVASVSDLLKSECAIL"
     gene            6017..7039
                     /locus_tag="PLT86_04425"
     CDS             6017..7039
                     /locus_tag="PLT86_04425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOT35895.1"
                     /translation="MNAATQGLPHGPRPAPLDFEAELVKLEDRITDMRKWADEYSKTG
                     QDPAIAQEIAKLEARANEMRAAIYRNLTHWQRVQVARHRDRPYTLDYINLLMEDFSEL
                     HGDRHFADDAAMVCGIARFRGRSVALVGQQKGRTVEERIKRNWGMAGPEGYRKALRIM
                     KLAEKFRLPVISLVDTPGAAPGVGAEERGQAEAIARNLFETSRLQTPILVAIIGEGAS
                     GGALGIGVGDRVLMMENCWYSVISPEGCAQILWRTADAKEQAAAAMKISAQDLHALGL
                     IDEVVPEPVGGAHRAPEEAARILGDRLEENLNLLAAIPPTELLERRRNRLYAYGQWEE
                     REVASR"
     gene            7036..7206
                     /locus_tag="PLT86_04430"
     CDS             7036..7206
                     /locus_tag="PLT86_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOT35896.1"
                     /translation="MRKELLDILVCPKCKGDLDYSEAEQTLTCRACRLRYEIKNGIPI
                     MLVEKAIPIPSE"
     gene            7216..8553
                     /locus_tag="PLT86_04435"
     CDS             7216..8553
                     /locus_tag="PLT86_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT35897.1"
                     /translation="MPHTKSIPYARRLPVRAEVEVLVAGGGPAGVAAAVTAARQGRVV
                     HILEAHSCFGGMGTAGLVPAFMQFTDGVHFLAGGIGKEILDELQRREGTYPHDGTGIR
                     AEVLKRVYDDMVSDAGVDFTFHAHVVDVLWEERGTVSTVVCAGKTGLFAVEAKTFVDC
                     TGDGDVCAFAGAPYEKGDETGAMMPGTLCSLWADIDWEAVKAGGITGEERNKRLEQAL
                     KDGVFTLEDRHLPGIWPVGDHVGGGNVGHTFEVDGTDEESLTAAYVWGRKSMTEYERF
                     YKEYLKGYEKMALVATGSLLGVRETRRIMGDYVLTVDDYTNRAVFEDEIGRYSYPVDI
                     HIAKPDRKSYDAFMKEHTTLRLGKGESYGIPYRILTPKGLKNVLVAGRCVSTDRQAQS
                     SIRVMPGCYITGQAAGMAAALMVETGKDSRGIEVRELQKRLKAMGGYLPNFAG"
     gene            8674..9426
                     /locus_tag="PLT86_04440"
     CDS             8674..9426
                     /locus_tag="PLT86_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT35898.1"
                     /translation="MPTMSMPWGTMAYTDSGDAGAGSPTMLFLHGTGCDAGDWDAVIE
                     ALPPTVRKLTLEFRGHGRSSVPVGPFTFSDLADDVVQFIEAMKLRRVILVGHSLGGMV
                     GMQVARQGDDTTRCGDVAVLVLLEGWTRLGCMGKAFEGERFYGHLDASAIEAITRKRD
                     ATMARFPAETWEHFWRSVREFDGSAFLSEARIPILEVYGGMGRVPGAEHELRVPENLY
                     IQTMWIPDAGHYLPIEKPFEVAKICADMAEFV"
     gene            9504..10829
                     /locus_tag="PLT86_04445"
     CDS             9504..10829
                     /locus_tag="PLT86_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT35899.1"
                     /translation="MQLSMFDTSVVSSRTATNGSANKVASDDRRFHDWYRFVLSFPPH
                     LVRDYFQRFGLQPGDTVLDPFCGTGTTLVEAKLNGIQSIGIEANSMAHFASSVKVDWC
                     IDPNELRENSYWVAHMANRQVAEAPCGGLRTLAAGAYSLLLKGSISPVPMHKSLILLE
                     SIRTNGHPNLRRHQLLAFAKALVVSASNLHFGPEVGVLKKKRTDAPVVESWLAEIDNM
                     VYALTSVSCRTTPAMVHNADAREITNLLRRESIDGVFTSPPYPNEKDYTRTTRLESVM
                     LGFINNKDELRLMKSKLLRSNTRNVYKGDSDDEWITGFPAIQQIADEIEARRLALGKT
                     SGFERQYARVTKLYFGGMARHFSTLRSVLKSGAYLGYVVGDQASYLQVMIRTGELLAE
                     IARSLGYRVVGIDLFRTRLATATRKQLREEVVVLQWPGFAPGEANARKA"
     gene            10813..11619
                     /locus_tag="PLT86_04450"
     CDS             10813..11619
                     /locus_tag="PLT86_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOT35900.1"
                     /translation="MPEKPNRYSSVIAEVFRRKYQEGAQRVSFEREELAAVAAELGVK
                     LPKNLGDIVYSFRYRTRLPESIMRSAPSGLEWIIRATGQAKYEFSLTAMPRFVPNASL
                     AETKIPDATPGIIAKFASGDEQRLLARLRYNRLVDVFTGVTCYSLQSHFRTTAPGIGQ
                     VETDEVYLGVDRRGAQYVIPVQAKGGTDQIGVIQIEQDIAICASKFPLLICRPVAAQF
                     LEKNLIAMFLFEATEHRIAVISEKHYRLVPPDNLTKEDLESYGRRPIENI"
     gene            11677..12750
                     /gene="hisC"
                     /locus_tag="PLT86_04455"
     CDS             11677..12750
                     /gene="hisC"
                     /locus_tag="PLT86_04455"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT35901.1"
                     /translation="MFDLLDPIKPLVRTLPAYTLAALETKIKLNQNENPYEIPADLKE
                     EVLRFALERPWGRYPDFVPDEFLALLAAHVGWVREGMLAGNGSNELIQAILAVTCGPG
                     TKVLIVQPTFTLYKLLSRINGAEVVEVSLRREDLAYDIPAVCAAIDENEPGVVILCSP
                     NNPTGSALTLGEWRQVCERAPGLVVADQAYVEFGGDCVAPLLKEFPNLVMLRTFSKAG
                     TLAGLRVGYCAASPDIAEQIAKAKLPYNLNFFSMAVAAMVVRNWHRFEPVIARIRSER
                     ERVYAALGNMAGVRVYPSAANFLLFETDRPPAEVFRGVYDRGVLIRDVSKYPLLGKAL
                     RVSIGTPEENDAFLSALRATLGA"
     gene            12759..13709
                     /locus_tag="PLT86_04460"
     CDS             12759..13709
                     /locus_tag="PLT86_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT35902.1"
                     /translation="MPDQAPRHVADIVPGETVTMFLALRGKQLREHEGKPFLRFEFAD
                     RTGRIMAVTSDNMDGFWHAADQNDVVKVRGVVGTWNGKKTLRIQQMRRAKPGEFDAGD
                     FLPRYEGDTDALWKELVAYAESVTDPPLRSVLGNLVDSEEMRGRFLQAPAGKLWHHTY
                     LGGLAEHTANVVALVDSACKRYPLADRNLAIAGGLLHDIGKIEAFDVSTTIEYGDAGR
                     LVSHIVLGERIVAEWCRCVPELPAGHADKLCHIVLAHELSGDQRSPISPMTLEAAIVA
                     AANQLDATAGAFARILTRERDTGQAWSAWVNTLERHVFLK"
     gene            13911..16418
                     /gene="topA"
                     /locus_tag="PLT86_04465"
     CDS             13911..16418
                     /gene="topA"
                     /locus_tag="PLT86_04465"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT35903.1"
                     /translation="MPRSLVIVESPAKARTINKVLGREFDVRSSMGHVRDLPENSLGV
                     NVEEDFLPQYEVLPSKRKIVNELRMAGKNAERVYLATDPDREGEAIAWHIAEVMRLGK
                     NRTRRITFHEITPRAIKEALDHPTELDMHKVDAQQARRVLDRLVGYQVSPLLWQTVRK
                     GLSAGRVQSVALRMVCEREEEVLAFKPREYWTVLVALKNGAVAFEADLWKVDGKLAKI
                     PDEAHAKALVEELTRGQFDVVSLERKRQKRRAFPPFITSTLQQEAARKLGFSTRMTMS
                     VAQELYEGVEVKGELTGLITYMRTDSTHLAPEAVSAARQYIAETYGKEYVPARPNVFR
                     NSKSAQEAHEAIRPTSMDRPPEAVQGELSRDQFRLYQLIWNRFVSSQMEAQELDVTEL
                     VAAGGRFQLRTTASVVAFDGFTRVYLEGQDEEKEEQEHPIPRELIAAWDRTEQGAKRS
                     AEPLLNGYAPAKVLPEQHFTKPPSRFTEATLVKELESRGIGRPSTYAQIISTILDREY
                     VARKEGKLVPTELGKIVNRILSSQFPEIFNSEFTAKMEEELDRVEQGSDHWKSVLKEF
                     YSGFEATLSSAMKRRSEIKKLTVEQSDRVCEKCGKPMVVRLGPRGRFLACSGFPECRN
                     TVALDTEAAPPEIPVPDGVTCPKCGSGMTVRNGRYGVYLACEKYPSCRGARPIPTGVP
                     CPRPGCNGELVIRTSKRGKHYYACDQAGCTVLYWDKPVPLPEPDPATGLRFQLEKTRR
                     DGTTIVSPGVYPPPALRRRSTAAENRSGARTGSRYSRSARAGTKAAEAEPTSGRRGTT
                     SRKSSRSSSAAVGATPSAAGKRSSAKRSAGGTGNARK"
     gene            16452..17357
                     /locus_tag="PLT86_04470"
     CDS             16452..17357
                     /locus_tag="PLT86_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOT35904.1"
                     /translation="MRAAIGDFIQYLIGERNYSQHTARAYRADLLQFAEYLEEELAHP
                     PALRDLTRGYVRTYVTWLMETGHKQRTIARKLASIRSFQTYLQRHDLVSEQDWGRLAP
                     RQQQRTLPTVLDADQLALLMTLPPNEDPTGVRDRAIMELLYSSGLRVSELVALDLEHL
                     DIAEGLVRVRGKGNKERIVPVGTIALEALDRYIREFRPLLIPPALRRRKFSPLFVSKL
                     GRRLSVERVRAMVTGYLRQVSNSEHLGPHLLRHACATHLVERGAGLRDVQEMLGHSSV
                     TSTQIYVSVASRRLTQIYGKSHPRA"
     gene            17570..18109
                     /gene="hslV"
                     /locus_tag="PLT86_04475"
     CDS             17570..18109
                     /gene="hslV"
                     /locus_tag="PLT86_04475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041467627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOT35905.1"
                     /translation="MNYPEIHSTTVLGIVRDGVAVIGGDGQVTLGNTVVKRSTVKVRK
                     LANNTVLAGFAGGAADAFALLEHFEGKLEETRGNLPRAALSLARDWRTDKFLRRLEAF
                     LIVMDKQQGFILSGTGDVIQPEDGVMAIGSGASYALAAAKAYLDGSGLPVREIVERSL
                     RIASDICIYTNATLTIEEL"
     gene            complement(18141..18488)
                     /locus_tag="PLT86_04480"
     CDS             complement(18141..18488)
                     /locus_tag="PLT86_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35906.1"
                     /translation="MLELKHEPVLPIRQFVGRLLGFTAAAVLLVVIALGIGVGGYYWI
                     AGLGFVDALLNASMILGGMGPVDPLRGDAAKLFASAYALFSGLVFLTVAAMLFTPLVH
                     RMLHRFHVDETGQ"
     gene            complement(18544..>19610)
                     /locus_tag="PLT86_04485"
     CDS             complement(18544..>19610)
                     /locus_tag="PLT86_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35907.1"
                     /translation="SGTTHSRCAGGGGDPGGAGQVNGGTASGDKEPGAVDWLSPQVWR
                     RDSDVPCVSLGPPGAFDDAHIFAPCVAFEDGLFRMWYCGSRGTVEGRVFRMGFATSFD
                     GVNFTKHPASPVLSFGDTRSILTPTLLRHPGGSLCREDGCLRMWFSSTDFPAGDGVHT
                     LHETTGVDGLTWSPPSRPLLHNLYAPTIIKQDSVYRMWYTDVVADPWCFRYAESGDGT
                     RWDVVPQPVMVLDQPWEHGRLFYPTVLVADGLHLMWYGSYSHRKGEETRTALGFAVSK
                     DGITWEKSPSNPVFGPDPAHAWESNFTTSQSVLWLPDRSLRIWYASRPKPPFTHKYFA
                     IGTARCIPGTAGRMRRPEAG"
BASE COUNT         3898 a         5750 c         6372 g         3490 t
ORIGIN      
        1 ccctcgaccg cgcgaagcag gccctcgatg atgttgccgc ccgcaagccg ggggtcatca
       61 aagcgatggt ggtgaacggg tagggcgccg ggaagtgtcg cctggattcc cgcctgcgcg
      121 ggaatgacgg gaggggaagg gcagacccct gtggctgccg cttcgatccg tgtgcgcttt
      181 ggaggaagca gggcaggcat gaatgcctgc cccaccatgc gcggacggcg ggagttcggc
      241 agagtacagg tcctatgggt cgcataggac gcatatgccg cggatgccac tcatgcgtcc
      301 ggtcgcccgg atacagcgct ccgcccagtt tccaatcacc gccccgcggt tcggtcattc
      361 ccgcgcgggc gggaatccag gaaggggaac ggggatgtcc gccacccagg tgaccgggca
      421 ggaggaaggc ggtcactgag cctgtcgaag tgacggtcgc agcacgctgg cagcgcgggc
      481 acgacgaagc gtgcccctcc attcggggcg gaccttacgg gagtctgcca tattataggt
      541 cctatgggta gcataggaca gatacggcgc gggttccctc agaatgactc gctggcgccc
      601 gacccgaccg tcattctgaa cggagtgaag aatctcttcg gggccgactg gcacgctccg
      661 gtcgtctcga tacatcgtcc gccggaaacc cggcggccgc cactcgacga ccgggaagcc
      721 gcttcccgga ggaggagatc ggttcgttcg ggcatgcagc cgttcccgtt cccgattttc
      781 cgtgtggttc gtgtggttcg tggttcctca cgccgttacg taaacctcac ctccggccac
      841 ttcggctcgc cctccaccgt gatctcgatt accgacacat tcacatccgg cgcatactgc
      901 ggcaggcctc ggagccagac gcgatcgccc ttctgctcga cgatcaaccg ttccccggtc
      961 gccagcagct tcgccgagag cacccggttg ccgcaccagc cgaacgacgc cgttgagccg
     1021 ggccagtgag cggagaggag gtacagcctg tccccgacgc gggtgggggt ggcgaagctg
     1081 tgggtgacga gcggggcccg cgttgcgccg tagattgcct tgccgttggt gcgcatccac
     1141 tccccgacca cgcgcaggcg ctccaccgcg ggttcgggga tccgcccctc cgcgtccggg
     1201 ccgacgttca gcagcagatt ccctccctgc gacgcgcact ggatcagcat cccgagaatc
     1261 tgccgcacgg acttgtaatt cttatcgccc gcagtgtagc cccacgtgtc gttcatggtc
     1321 atgcaggcct cccacgggcg gtcttgcggg gcgatgtgct gctcgggagt gccgaaatcc
     1381 tccggaagac ctgagcggtc gttgatgacg atgttcggct gcagcgaccg caccatggcg
     1441 ttcagctcct ccgagcgcca gattccggcg gggaagccac cgtcgtacca caggatatcc
     1501 accgtgccgt agttcgtcat gagctcacgc acctgagcgt gggcctgctc caccatgggt
     1561 tggtacacgc ttttgtcctc gcgcatgggc aggtgcggca actggtgggg gaagcgccag
     1621 tcctcaagcg agtagtagaa ccccatacgc atacccagcg cgtggcaggc atcggcgtat
     1681 tcggcgatga ggtcgcgttt cgcggcggtt ttcggcgcgg tgaagtcgga gaccttgctg
     1741 tcgaacaggc agaaaccgtc gtggtggcgc gtggtcagga ccatgtactt catgccggcg
     1801 tcctgcgcga gcttcaccca ctcccgcgcg tggtagtgcc gggcggtgaa accgtcggcg
     1861 aagtgggcgt attcctcctt cgggatgttt tccacgtaca tcacccactc gttccgcgcg
     1921 ggaacggcat acacgcccca gtggatgaac atgccgaacg tggcttcgcg ccaccatttc
     1981 atgtggtcgg ggatagcgtc tgcgctcatt gtgcgcctcc gtgtgagggg aaagacctgc
     2041 gaaccgggac agggaatggt acggcagagg aggcgtgcgc gggaaatggc tagcaaaccg
     2101 tgcggctggc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggatgatg
     2221 ccgttccgca cggcgggatt gcgccagttg tcgaagtccg ccaggacggc gtccactgcc
     2281 gggcttcgca gctttcgcag cgccgggtgg aggcggtcca tgacaggccc gcagtaggca
     2341 tccacgaacc cggtgcgctc cgtccactcc cgccacgacg caaccagacc gcgcacctcg
     2401 ggtgtgccgt cgccgcccgc gtcaaccgcc gccgcgaagt cgctcagatc ggcagaaaac
     2461 gccagttgca ggacaagatc gccaaggaag cgcgtgggat cgtgcatcag cagccctgag
     2521 aactcgccgc ggggcagttg tcgtgcctcc ccgcgggtga gttccttcgc cagacgacgc
     2581 gcaggctcga tcgcgcgatc cgccagtgcc ttccacgcct ccgcgatgag gctgccctga
     2641 ccgggaatca gcttctccgc aaagcccgcc agatcgacgt cttcgagtct gccgccccgg
     2701 gcgaagtggg cgaacaggta ggtgttgggg agttggacga tgtgggtctg cgcattggcc
     2761 atgcagccga ggtagggccg gccccacggg tatcccgaaa gcgctttttc gacgccagtg
     2821 ggatcgcagt ttgtcagtgg aacggtcggc tctccttcaa cggcgccgta ggggaagtag
     2881 acacatcgct cttcgacgcc caggcgttcg gcaagccgcc ggtggctgtc ccagcagaca
     2941 agaaggcgcc acgggtcgtc aggcgtggtg cagaggtctt ccaccacctc cgcccagtcg
     3001 cttgggttga cttcgagctt tgcgtccccg cccttttccg cctgttccca gaaccggttg
     3061 tagctctccc acccaaccca gagccagtac cagagcatcg ccttcgggtt cgcgtcgcgg
     3121 agcagcctga ggtgcctgcg caggatggca gtgaattcgg cgttcgttga cccggcatac
     3181 ccgcccgggt cgctatcaat gacgacgatg ccgtccgcat gcgcaaggta gcggtacagc
     3241 gacgcgcgga accgcatcag cctgtccatg gcggaaggat cggcagggtt caggcgcagg
     3301 tcggtcctga agaacgggcg cgtttcgaat ggatactccg cggcgtgcgt gttcccgatt
     3361 gtgttcgcgc cgaacgtgat gagcaccgtc atgccgaact cgtgctggag catggtgatt
     3421 acgttgccga gtttctccag atgggcaacg tcgctcgccg tcgggggatc cggcattgtc
     3481 tccgtaagcg gccagatcat gacgaggtta taaccgagcg cgcggagccc ggaggcgtag
     3541 gcgcgccagt cctcaagcga ccacgtccgc gcggcatacg ggtaggcata accccagtgc
     3601 atgtgcacgt acatgccgag aagcgggctc atcttcactc cggggttctg cctcccgcca
     3661 tccggggcac gggagagccg ggtcaaagga tcgcgcattc cgatttgaga aggtcggaga
     3721 cggaggccac ggcatccggc ggacccatga caagaagaac gtcgcctgcg tgaatcgtct
     3781 cctcgccccc ggggttcggg atggtcttgt ccccgcgccg gatgccgaga atagtgattc
     3841 cggtgcgact tcgcagaccc agttcggcga gtgtctgccc tgccatccgg cacccttcgt
     3901 ccacggggaa cgtgctgact tccacatccc gcaaagaagt ctggatttct gagagcgcgg
     3961 taaaatcgcc agagaagctc cggaacatgt cgtacccgcc ggcgcggatt gtccggatga
     4021 agttctcgat ctgctcccgg ggaatcaggt atttgcgcag aacgcgggag aagatctcca
     4081 ccgacgtttc gaattcttcc ggaatcactt cgtcggctcc aagtttatac aacggcgcga
     4141 cttcggtaac aaaccgcgta cgggcgatga tgtagacgga cgggttcatc cggcgtgcgt
     4201 tttcgacgat ccggcgcgtg ccggttgcgt ccggcgtcgc gaccacgagc acccgcgcct
     4261 gcaggatgcc cgcgtgttct agcacgaccg gctgcgagcc atcgccgaaa aggatggact
     4321 ctccgtttgc ccgttcacgc cggacggttt ccggattcat ctcgatgata acgtagggaa
     4381 tcagcgcctc cgcagccgca ctggcgacgt tccgcccgtt cacgccgtat ccgatgatga
     4441 tcagatggtc ggtgaggcct gcgtttccgt cgtcctctcg cgcgaatgat ccggctcgca
     4501 cccgcttcgg caagggcagg cggaggagtt gatccgcgat atgcggcgcc gcctgtatga
     4561 ggaagggcgt tgccgccatc gtaatcactg acacggtcag gaaatactga taaccggagg
     4621 gctcgagcag cccgaattga agcccggacg cagcaagaat gaaggagaac tctccgatct
     4681 ggccgaggcc cagggccacc ccgatcgtcg tgcggaggga atagccgagg agcgcggtgg
     4741 cggccccggc aagaagcacc ttgatgatga tggaaccaca tgccatcgcg gcaacggcga
     4801 ttgcgtggtc gaacaagaac ccgatatcga ggagcatccc gacggagacg aaaaacaggt
     4861 tcgaaaagat gacgcgaaac ggtatgacgt cgcccagtgc ctgctggctg tattccgact
     4921 ccgagatgat gataccggcg agaaacgcgc cgagcgcggg cgccagtccg atggagtacg
     4981 tcaaccacgt gatggcgagg cagatgacca ccacggcgaa caggaacaac tcgccgcttc
     5041 gcgttcgcgc tatctggtag agcacatacg gcacgaccca tcgggcgctc acgatgacga
     5101 tggcaattac ccccaacccc tgggccgcca tggtgaggag cgacccggcg gattgcgcgg
     5161 tcgcttccgc aaggagggga agggaaaaga gcatcgggac ggtaatgatg tcctgaaaca
     5221 cgagaatgcc gatgacgatg cggccgtgga gggaatctgc ttccgccctg tcctgcaggc
     5281 ttttgatggc aatcgccgtg ctgctgagcg agaggagaaa accggcaaga acggcgaccg
     5341 ggggcggctg acccagcgcg cgggccgcca ggtaccccgc agcgcccgtc gcgacgatct
     5401 ggattgttcc gccgaccagc acggtgcgcc tgactctgag cgcgtgctcc agcgagaaac
     5461 tcagcccgac ggtgaacaac agggcgacga tgccgacttc ggaaatgctt tccactcgcg
     5521 caacatcgct caccagcccc agcgcgtgcg gtccggccac gatgccggtg aggaagaagc
     5581 cgacgagaga gggaacgtgc aggcgctcgc agataaacac cacggcgacg gacaacgcga
     5641 agatgatgag gatgtcactc aggagcgttg cgtgcacatt cggcctttcg tgtcgcagtt
     5701 ggatgctgcg ggaacacgca gggggaaatt acgattccgg gccccgccgg gcaagttgcg
     5761 cggcgatccg gcgcgggtcg gacgcgctcc gttgctgttc ggaaaaaaac tgcttacccc
     5821 cttgacacga acacgccgtt ttacctcacc tgaaaaaggg cgtgcactcc aggatcaaag
     5881 ggcagggcgg gccggcgctt tcgcccgcgc ctttgcatcc cgtgcgggct tgtctactct
     5941 ttattccacc ggtgtgtggt cacgcgttcg ggggaccagc cggaatcacg cgacgattgc
     6001 cacacaagga gcgcgcatga acgccgcaac gcagggtctg ccgcacggac cccgtcccgc
     6061 gccgctggat tttgaggcgg aactcgtgaa gcttgaagat cggatcaccg acatgagaaa
     6121 atgggccgac gaatactcga aaacggggca ggatccggcc attgcgcagg aaatcgccaa
     6181 gctggaggct cgcgcgaacg agatgcgcgc ggcgatttac cgcaatctca cgcactggca
     6241 gcgggtgcag gtcgcccgcc atcgcgacag gccttacaca ctcgactaca tcaacctgct
     6301 gatggaggac ttcagcgagc tgcacgggga ccggcacttt gcggacgacg cagcgatggt
     6361 atgcggcatt gcccggttcc gcgggcggtc ggttgccctc gtggggcagc agaagggacg
     6421 tacggtcgag gagaggatca agcgcaactg gggcatggcg ggtccggagg ggtaccgcaa
     6481 ggcgctgcgc atcatgaaac tggcggagaa gttccgtctg ccggtcatat cgctggtaga
     6541 tactcccggg gcggcgccgg gcgtcggggc ggaagaacgc ggtcaggccg aggcaattgc
     6601 gcggaacctg ttcgagacgt cgcgtctgca gacgccgata cttgtcgcca tcatcggaga
     6661 aggtgcctcc gggggagcgc tcggaatcgg cgtcggagat cgagtgctca tgatggagaa
     6721 ctgctggtat tcggtcatca gcccggaggg gtgcgctcag atcctctggc gcacggccga
     6781 cgcgaaggaa caggccgccg ccgcgatgaa gatcagcgcg caggacctgc acgcgctggg
     6841 actgattgat gaggtggtgc ccgagccggt cggcggcgcg caccgtgcac cggaagaggc
     6901 cgcgcgaatc ctcggcgacc ggctggagga gaacctgaac ctgctggcag ccattccgcc
     6961 aacggagctg cttgagcgcc ggcgcaaccg gctgtacgcc tacggtcagt gggaagaaag
     7021 ggaggtggcg tcgcgatgag gaaagagctg ctcgatatcc ttgtgtgccc caaatgcaaa
     7081 ggcgacctcg actattctga ggcggaacag acgcttacgt gccgggcatg ccgccttcgg
     7141 tacgagatca aaaacggcat tccgatcatg ctggtggaga aggcgatccc gatcccctcg
     7201 gaataggagg cttccatgcc acacacaaag tccattccgt acgccagacg gcttcccgtc
     7261 cgtgcggagg ttgaggtgct ggttgcgggt ggtggccctg ccggcgtggc ggcggccgtc
     7321 acggcagcgc gtcaggggcg cgtggtgcat atccttgagg cgcattcctg tttcggaggc
     7381 atgggcacgg cgggccttgt gccggccttc atgcagttca cggacggcgt ccatttcctc
     7441 gcgggaggca tcggcaagga gatacttgac gaactgcagc gccgggaagg cacgtacccg
     7501 cacgacggca cgggcatccg ggcggaagtg ctgaagcgcg tgtatgatga catggtgagc
     7561 gacgcgggcg tggatttcac cttccatgca cacgtggtgg acgtgctgtg ggaggagcgg
     7621 ggtacggttt ccaccgtcgt gtgtgcggga aaaacgggtc tgttcgcggt ggaagcgaaa
     7681 acgttcgtgg attgcaccgg tgatggcgac gtgtgtgcgt ttgccggcgc gccgtacgaa
     7741 aagggcgacg aaactggggc gatgatgccg ggcacgctgt gcagtctctg ggccgatatt
     7801 gactgggaag cggtgaaggc gggcggcatc accggggaag aacgcaacaa acggctcgaa
     7861 caggccctca aggatggtgt gttcaccctc gaagatcgcc atcttcccgg catctggccg
     7921 gtgggggatc acgtcggtgg cggcaatgtc gggcacacgt ttgaggtgga cggaacggac
     7981 gaggagtcgc tcacggcggc gtacgtctgg ggccggaaat ccatgaccga atacgagcgg
     8041 ttctacaagg agtacctgaa aggctacgag aagatggcac tcgtagctac ggggtcgctg
     8101 ctgggtgtgc gcgagacgcg gcgcatcatg ggtgactacg tgctgaccgt ggatgattac
     8161 acgaacagag cagtttttga ggacgaaatc ggtcgttact catatccggt ggatatccat
     8221 atcgcaaagc cggacaggaa aagctacgac gccttcatga aagagcacac gacgctccgc
     8281 ctcgggaaag gggagagcta cggtattccg taccgcatcc tcacgccgaa aggactgaaa
     8341 aacgtcctgg tggccgggcg gtgcgtgagc acggacaggc aggcgcagag ctccatccgg
     8401 gtgatgccgg ggtgttacat caccggacag gcagcgggga tggcggccgc gctgatggtg
     8461 gagacgggga aagattcccg cggcatcgag gtgcgggaac tgcagaagcg cctgaaagcg
     8521 atgggcggat atctgccgaa tttcgcgggg taaccctgtt ggagggacgc gtttcctcgc
     8581 gtccgttttg cagtcgtcgt gactgttccc gggatagaga ttcttcactg cgttcagaat
     8641 gacgaaaagg ctcacattag cgggaaaccg cccatgccaa ccatgagcat gccgtgggga
     8701 accatggcgt acacggactc cggagacgcg ggagccgggt cgccgaccat gttgtttctt
     8761 cacggaaccg ggtgcgatgc cggggactgg gatgccgtga tcgaggcgct tccacccacg
     8821 gtgcggaagc tgacgctgga attccgcggg cacgggcgga gcagtgtgcc ggtgggaccg
     8881 ttcacgttca gcgacctcgc ggacgacgtg gttcagttca tcgaggcgat gaagctccgg
     8941 cgggtgattc tggtggggca cagccttggc ggcatggtgg gaatgcaggt tgcacggcag
     9001 ggcgacgata ccacgcgctg cggggacgtc gccgtacttg tgctgctgga aggctggacg
     9061 cggctggggt gcatggggaa ggcgtttgag ggagagcgct tctacggcca ccttgatgct
     9121 tccgccatcg aagcgataac gcggaaacgc gacgcgacga tggcccggtt ccccgcagag
     9181 acgtgggagc atttctggcg atccgtgcgt gagtttgacg ggtccgcctt cctgagcgaa
     9241 gcacgtatcc cgattctgga ggtatacggc gggatgggcc gcgtgccggg ggcggaacac
     9301 gagctgcggg tgccggagaa tctctatatt caaaccatgt ggatacccga tgcggggcat
     9361 tatctcccca tcgaaaaacc gtttgaagtt gcgaagatct gcgccgacat ggcggagttt
     9421 gtgtaattga gtcagtggtt gctcctcgtc acgttcccgc gatgcgtggc gttgagttcg
     9481 cgcattggcg gcaaggggat acagtgcaac tctcgatgtt tgatacgtcc gtcgtttcct
     9541 cgagaacggc gaccaacgga agcgccaaca aggttgcgtc agacgatagg cgctttcatg
     9601 attggtaccg attcgttctg tcgttccccc cccacctggt gcgggattac ttccaacgct
     9661 tcggcctgca accaggcgat acggttctcg acccattctg cggaaccgga acaacgctgg
     9721 tcgaagcaaa actgaacgga attcagagca ttggcatcga agcgaattca atggctcact
     9781 tcgccagttc ggtcaaggta gactggtgta tcgacccgaa cgagcttcgc gaaaactcgt
     9841 attgggtggc gcacatggcc aatagacagg ttgcagaggc gccatgcgga ggacttcgca
     9901 cccttgcggc aggggcatac tctttgcttc tcaaggggtc catcagccct gttccgatgc
     9961 acaaatcact catccttttg gagagcatcc ggactaacgg ccatccgaac ttgcggcgtc
    10021 accagttgct cgctttcgcg aaagcccttg ttgtaagtgc cagcaatctg catttcggcc
    10081 cggaagtggg tgtgttgaaa aaaaaacgaa ccgatgcacc ggtggtggaa tcttggttgg
    10141 cagaaattga caacatggtc tacgccctca cgagcgtgtc atgtcgaacg acgccagcca
    10201 tggttcacaa cgcagacgcg cgcgagataa cgaacctatt gcgccgtgaa agtatcgatg
    10261 gcgtctttac ttcgccgccg tacccgaacg aaaaagacta cacgcgaact actcggctgg
    10321 agtcggtcat gctcgggttc atcaataaca aagatgaact tcggttgatg aagagcaagc
    10381 tattgagatc taatacgcgg aacgtttata agggtgacag cgacgacgaa tggataacag
    10441 gtttcccagc aatacaacag atagcagacg aaatcgaagc gaggcgcctg gcgctcggca
    10501 agacatctgg gttcgaacgg cagtatgccc gcgtaaccaa actctatttt ggaggcatgg
    10561 cgcgccattt ttcgacgttg cggtcggtac tcaaatccgg ggcctatctg ggctacgtcg
    10621 ttggcgacca agcctcatac ctgcaggtga tgatccggac aggcgaattg ctcgctgaaa
    10681 tcgctcgatc attgggatac cgtgtggttg ggatagacct tttccggacc cgtctcgcga
    10741 ccgccaccag gaagcagctg cgggaagaag tagttgtgtt gcagtggccc ggatttgctc
    10801 caggggaagc caatgcccga aaagcctaat cgatattcat cagtgattgc ggaggtgttc
    10861 cgcaggaaat accaggaggg agcgcaacgg gtctcgttcg agagggaaga gcttgccgca
    10921 gtggctgccg aactcggcgt gaagctgccc aaaaacttag gcgacattgt gtacagcttc
    10981 cggtacagga cgaggcttcc cgagtctata atgcgttcgg cgccgtccgg cctagaatgg
    11041 attatccgcg cgacgggaca agcgaagtac gagttctcgc taaccgccat gccgcgattt
    11101 gtgccaaatg catcactcgc tgagacgaag atccccgacg ccacgccggg catcatcgcg
    11161 aaattcgcat ccggtgatga gcaacgactt cttgccaggt tgaggtacaa caggctggtg
    11221 gatgttttta cgggggttac ttgttactcg ctgcaaagcc atttcagaac aactgcgcca
    11281 ggcatcgggc aggtggagac tgacgaggtg tatctcggcg ttgacaggcg gggagcacag
    11341 tacgttatcc ctgttcaggc gaagggagga accgaccaga tcggcgtgat acagatcgag
    11401 caggacatcg cgatctgtgc gtcaaaattc ccactcctga tctgccgacc ggtagctgcg
    11461 cagtttctcg agaagaacct catagcgatg ttcctgttcg aagcaacgga acacagaatc
    11521 gcagttatct cagaaaagca ttaccgtctt gtgcctccgg acaatttgac gaaagaagac
    11581 ttggagtctt acgggagacg gccgatcgag aacatctgaa cggttccgtc tcgccgacgc
    11641 agaaactgga ttttccgcca ccgcctgtca gaggaagtgt tcgatctctt ggacccgata
    11701 aagccacttg ttcgtacgct gccggcgtac acgctggcgg cgcttgagac gaagatcaag
    11761 ctgaaccaga acgaaaaccc gtacgaaatc ccggcggacc tcaaggagga ggtgttgcgc
    11821 ttcgcgctgg agcggccatg ggggcggtat cccgatttcg tgccggatga gttcctcgcc
    11881 cttctggcag cgcacgtcgg ctgggtgcgc gaggggatgc tggccgggaa cggctcgaac
    11941 gagctgattc aggctatcct cgcggttacg tgcgggcccg gcacgaaggt gctgattgtt
    12001 cagccgacgt tcacgctgta caagctcctg agccgcatca acggcgcgga ggtggtggag
    12061 gtgtcgctac gccgcgagga tctggcgtac gacattccgg cggtgtgcgc ggcgattgac
    12121 gaaaatgaac ccggggtagt gattctctgc tcgccaaata accctaccgg cagcgcgctc
    12181 acgctgggcg aatggcggca ggtatgcgag cgggcgcccg ggctggtggt ggcggatcag
    12241 gcgtatgtgg agttcggcgg ggactgcgta gcgccccttc tgaaggagtt cccgaacctt
    12301 gtaatgctgc gcacgttctc caaggcggga acgctggcgg gcctgcgcgt ggggtattgc
    12361 gccgcgtcgc ccgacatcgc ggaacagatc gccaaggcga aattgccgta caacctgaac
    12421 tttttctcga tggccgttgc cgccatggtg gtgcggaact ggcaccggtt cgaaccggtc
    12481 atcgcacgga tccgttcgga gcgggaacgg gtgtacgcgg cgctcgggaa catggcgggg
    12541 gtgcgcgtgt atccctccgc cgccaacttc ctcctgttcg aaaccgaccg accacctgcg
    12601 gaggtgttcc gtggtgtata cgaccggggc gtattgatcc gcgacgtaag caagtacccg
    12661 cttctcggga aggcgctgag ggtgagcatc ggcacgccgg aggaaaacga cgccttcctt
    12721 tccgcacttc gcgcgacatt gggtgcgtga ggcacgccat gcccgaccag gccccgcgtc
    12781 acgttgccga catcgttccg ggtgaaaccg tgacgatgtt cctcgccctg cgcgggaaac
    12841 aactgaggga gcacgagggg aagccgtttc ttcgtttcga gttcgccgac cgtaccgggc
    12901 ggatcatggc ggtgacaagc gacaacatgg acggcttctg gcacgccgca gaccagaacg
    12961 acgtggtgaa agtgcgcggg gttgtcggca cgtggaacgg taagaaaacg ctgcgcatcc
    13021 agcagatgcg gcgcgcaaag ccgggggaat tcgatgcggg ggacttcctt ccccgctacg
    13081 aaggggatac ggacgcgctc tggaaggagc ttgtcgcgta tgcggaatcg gttaccgacc
    13141 cgccacttcg ctccgtgctg ggtaatctgg tagattcgga agagatgcgt ggccggttcc
    13201 tgcaggcgcc cgcgggaaaa ctctggcacc acacgtacct cggaggcttg gcggaacata
    13261 cggcgaatgt ggttgccctt gtggattcgg cgtgcaaacg atatcctctt gctgacagga
    13321 acctcgccat cgccggcggg cttcttcatg atatcgggaa gattgaagcg ttcgacgtct
    13381 ccaccacaat cgaatacggg gatgccgggc gccttgtgag ccatattgtc ttgggagagc
    13441 gcatcgtcgc ggagtggtgc cgttgcgttc ccgaacttcc cgcgggccac gccgacaagc
    13501 tgtgccatat cgtgttggca cacgagcttt ccggcgatca acgctctccc atatcgccca
    13561 tgacgctgga ggccgcgatc gttgccgcgg cgaaccagct tgacgccacg gccggcgcgt
    13621 tcgcccggat tctcacgaga gaacgcgata ccgggcaagc ctggtcggcc tgggtgaata
    13681 cactcgagcg gcacgtattt ctgaaatgac catgccgtgg cgacggttcc cgcatgttcc
    13741 gcggaacgcc agatcgccga catcagacga gacgcagaag agtcgctttc acagccgaga
    13801 cttgccgtcc tcctgttcgc cgtctattat cagaaagatt gtaacccctt atcctccgcg
    13861 ctgatagtaa tctcaacgcc gatcgcccga accgcgattc tggagtaacc atgccacgtt
    13921 cgcttgtcat tgttgaatca cccgcaaaag cccgcaccat caacaaagtc cttggacgcg
    13981 aattcgacgt tcgttcctcc atgggccatg tccgggatct tcctgagaac tcgctcgggg
    14041 tgaacgtgga ggaggatttc ctcccgcaat acgaagtgct tcccagcaag cggaaaatag
    14101 tgaacgagct gcgcatggcc ggaaagaacg cggaacgggt ataccttgcg acggaccccg
    14161 accgcgaggg agaggcgatc gcctggcaca ttgctgaagt catgcgactg gggaaaaacc
    14221 gcacgcgaag gatcaccttc cacgagatta cgccgcgggc aatcaaggag gcgctggacc
    14281 atcccaccga gctggatatg cacaaggtgg acgcccaaca ggcgcgccgc gtgctggatc
    14341 gcctggtcgg ataccaggtg agccctctcc tctggcagac cgtgcgcaag ggattgagcg
    14401 cggggcgcgt tcaatcggtg gcgttgcgca tggtatgcga gcgcgaggag gaggttctcg
    14461 cgttcaagcc gagggaatac tggacggtac ttgtcgcgct caagaacgga gccgttgcgt
    14521 tcgaagccga cctctggaag gttgacggca aactcgcgaa aatacccgac gaggcacacg
    14581 ccaaagcgct ggtggaggag cttacccgcg gtcagtttga tgtagtgagc ctcgaacgca
    14641 agcgccagaa acgccgcgcg ttcccgccgt tcattacaag cacgctccag caggaggcag
    14701 cccgcaagct cgggttctcc accagaatga ccatgagcgt tgcgcaggag ttgtacgaag
    14761 gcgttgaggt aaagggcgaa ctcaccggtc ttatcacgta tatgcggacc gattcgacgc
    14821 atttggcgcc ggaggcggtt tcggctgcgc ggcagtacat cgcggagacg tacggcaaag
    14881 agtacgttcc ggcgcggccg aatgtgttcc ggaacagtaa atctgcacag gaggcacatg
    14941 aggccatccg gccgacttcg atggatcggc cgccggaagc tgttcagggc gagctttccc
    15001 gcgatcagtt ccgcctgtac cagctgatct ggaaccgttt tgtctcctca cagatggagg
    15061 cgcaggaact cgacgtgacg gaacttgttg cggcgggcgg acgattccag cttcgcacga
    15121 ccgcgagtgt tgtcgcgttt gacgggttca cgcgcgtcta ccttgaggga caggacgaag
    15181 agaaagaaga acaggaacat ccgataccgc gtgaacttat cgcggcgtgg gaccgcacgg
    15241 aacagggcgc caaacgttcc gccgagccac ttctgaacgg gtatgcaccg gcaaaggtgc
    15301 tccccgagca gcatttcacc aagccgcctt cacggttcac cgaggcaacg ctggtcaaag
    15361 agctggaatc acggggaatt ggccgtccca gcacgtatgc tcagatcatc agcacgattc
    15421 tcgatcgcga gtatgtagcg cggaaagagg ggaagctggt tccgacagaa ctgggcaaaa
    15481 tcgtgaatcg tattctgtcc tcgcagtttc cagaaatctt caacagcgag ttcacggcga
    15541 agatggaaga ggagctggac cgcgtcgagc agggcagcga tcactggaaa tcggtgctga
    15601 aggagttcta cagcggcttt gaggcgacgc tttccagcgc aatgaaacgc cgatcggaga
    15661 tcaagaagct cacggtggaa caatctgacc gcgtgtgcga gaagtgcggc aagccgatgg
    15721 tcgtccggct ggggccgagg gggcgttttc tggcgtgttc gggctttccg gagtgtcgga
    15781 acacggttgc actggacacg gaagccgcgc cgccggaaat tccggtgccg gacggcgtca
    15841 catgcccgaa atgcgggagc gggatgacgg tgcgcaacgg gcgttacggg gtgtatcttg
    15901 cgtgcgagaa gtatccctcg tgccgtggcg cgcgtcctat tccgaccggg gtaccgtgcc
    15961 cgcggcccgg ttgcaacggg gaacttgtca tacggactag caagcgaggg aagcactact
    16021 acgcctgcga ccaggcgggc tgcaccgtcc tgtactggga taaaccggtt ccacttccgg
    16081 aacccgatcc ggcaaccggc ctccggttcc agcttgagaa aacgcggcgc gatggcacga
    16141 ccatcgtgtc tcccggtgtg tatccccctc cggcactgcg gcgacgaagt accgccgccg
    16201 aaaatcggtc gggtgcgcga acagggagca ggtattcgcg atcagcccgc gccggcacga
    16261 aggcggctga ggccgaaccg acgtccggtc ggcgtggaac gacatcgagg aaatcgagtc
    16321 gaagcagcag cgctgccgtt ggcgcgacac catctgcggc gggaaaaagg tcgtccgcga
    16381 aacggtcggc gggcgggacc ggcaacgcgc ggaagtaaac gatccgccgc ggcgccgggg
    16441 ggaaatatct gatgcgagcg gctatcggag atttcattca gtacctcatc ggcgagcgga
    16501 actactcgca gcacaccgcc cgtgcgtacc gcgccgacct tctgcagttc gccgagtacc
    16561 tcgaagagga actggcacac ccgccggcgc tgcgcgacct tacccgagga tacgtgcgga
    16621 cctacgtcac ctggttgatg gagaccgggc acaaacagcg cacgatcgcc cgcaaactcg
    16681 ccagtatccg atcgtttcag acatatcttc agcggcatga tctcgtcagc gagcaggact
    16741 gggggcggct tgcgccccgc cagcagcagc gcacgcttcc gacggtgctg gacgccgacc
    16801 aactggcgct ccttatgacg ttgcccccga atgaagaccc gacaggagtg cgcgaccggg
    16861 caatcatgga gctgttgtac tcctccgggc tgcgcgtgtc ggaactcgtc gcgctcgacc
    16921 tcgaacacct cgatattgcc gagggactgg tgcgggtgcg cgggaaaggg aacaaggagc
    16981 gcatagtccc cgtcggaacg atcgcgctgg aggcactgga ccgatacatc cgcgagttcc
    17041 gcccgctgct cattcctccc gcattgcgcc gccgcaagtt ttcgccgctc ttcgtgagca
    17101 aactcggcag gcggctcagt gtggagcgcg tccgcgcgat ggtgacagga tatctgcggc
    17161 aggtgagtaa ctcggaacat ctgggacctc atctgttgcg tcacgcctgc gcgactcatc
    17221 ttgtggagcg tggtgccggg ttgcgggatg ttcaggagat gctgggacac tcgagtgtga
    17281 cgtctacgca gatttacgta tccgtggcga gccggcggtt gacgcagatt tacgggaagt
    17341 cgcatccgag ggcttaggtc gccccgcgtg gtgataagat ggtggctacg gtttttgcgc
    17401 ggcgtttcga tacagcgcgg cgagatgccg cgccactcaa cgaccgcgcg agagaaacgc
    17461 cgctgactat tgcacgcggt ggctgagcga cctgcccccc gggcagcctg tcgaagcccc
    17521 gcgtgcagag tgatgcgcga taccatcagg aaagtcagaa aggtgacaca tgaactaccc
    17581 cgaaatccat tccaccaccg tcctcggcat cgtgcgggac ggggttgccg ttattggcgg
    17641 tgacggacag gtgacgctgg gcaatacggt ggtgaagcgg agcaccgtga aggtgcgcaa
    17701 actggccaac aacaccgttc tggcgggctt cgccggtggc gctgcggatg cgttcgccct
    17761 gctggagcac ttcgagggca agctggaaga aacccggggc aatcttccgc gggcggcgct
    17821 gtctctcgcc cgcgactggc gcaccgataa gttcctgcgc cggctcgagg cgttcctcat
    17881 cgtaatggac aaacagcagg gattcattct ctccggcacc ggggacgtca tccagccgga
    17941 ggacggagtg atggcgatcg gttcgggcgc ctcgtacgcg ctggcggccg cgaaggcgta
    18001 tctcgacggg tctggtctgc ctgtgcggga aatcgtggaa cgcagcctcc gcatcgcatc
    18061 ggacatctgc atctacacca acgccacact gacgattgag gagctgtgag gcggcaacgc
    18121 cacgccgcgc acaacgcacg tcactgaccg gtctcgtcca cgtggaaccg gtggagcatc
    18181 cggtgcacca gcggcgtaaa cagcatggca gcgaccgtga ggaacacgag gccgctgaac
    18241 agggcatacg cagacgcgaa gagtttggcc gcgtcgccgc gaaggggatc caccggcccc
    18301 ataccaccga gaatcatgga cgcgttcagg agcgcatcga caaaccccag accggcgatc
    18361 cagtagtatc cgcccacccc tattcccagc gcgatcacga ccagcagcac cgccgccgcc
    18421 gtgaaaccaa gcaatcggcc gacgaattgc cgaatcggca gtaccggttc atgtttcaat
    18481 tccagcatgg ttcacctcac aaaaaagcaa atcggtccgg acggggttcg cgtcagtctc
    18541 gcctcacccg gcctcgggcc gccgcatccg cccggccgtc cccggtatac accgcgccgt
    18601 gccgatcgcg aaatacttgt gcgtgaatgg tggcttcggg cgggatgcat accagatccg
    18661 cagtgaccgg tccggcagcc acagcaccga ttgactggtg gtgaagttgg actcccacgc
    18721 gtgggccgga tcggggccaa ataccgggtt tgacgggctc ttttcccacg taataccatc
    18781 cttactcacc gcaaacccga gcgccgtgcg cgtctcctct cccttgcgat gcgaatagct
    18841 tccgtaccac atcagatgga ggccgtcggc aaccagtacg gtcgggtaga agagccgccc
    18901 gtgctcccac ggctggtcca gcaccatgac cggttgtggc accacgtccc accgggtgcc
    18961 gtccccgctt tcggcgtagc gaaagcacca cgggtcagcc acgacgtcgg tataccacat
    19021 gcggtagacg gagtcctgtt tgatgatcgt gggggcatac aggttgtgga gcagcggcct
    19081 ggaaggcggc gaccaggtca ggccgtcgac gccggtggtt tcgtgcaggg tgtgcacgcc
    19141 gtcgccggcg ggaaaatcgg tgcttgaaaa ccacatgcgc aggcagccat cttcgcggca
    19201 cagggagccg cccgggtgcc gcagaagggt cggcgtgagg atggaccgcg tgtcgccgaa
    19261 tgacagcaca ggcgacgcgg ggtgcttcgt gaagttgaca ccgtcgaagg aggtggcaaa
    19321 ccccattctg aacacgcgtc cctcaacggt gccgcgtgaa ccgcagtacc acatgcggaa
    19381 gaggccgtcc tcgaacgcga cgcacggcgc gaagatgtgc gcgtcgtcaa atgcgcccgg
    19441 cggccccagt gacacgcagg gaacgtcaga atcacgccgc cagacttgcg ggctcaacca
    19501 atccactgca cctggttcct tgtctccaga agccgttcct ccgttcacct gccctgcgcc
    19561 gccaggatcg cctccaccac ctgcgcaccg agaatgcgtt gtcccgcttc
//
LOCUS       DAOSOO010000038        19517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2792_length_19517_cov_6.223307, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000038 DAOSOO010000000
VERSION     DAOSOO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19517
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_19517_cov_6.223307"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1185)
                     /locus_tag="PLT86_04490"
     CDS             complement(217..1185)
                     /locus_tag="PLT86_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOT35908.1"
                     /translation="MSKPIALQRVRGIVVYRNGALGDTIVTFPVLQNLRLAFPHVPLH
                     FVASKEVGLLAQTCGLTDSVVSADSEWARKWFAGDAAGMRALVEGADALIAFTSEDDE
                     LEETARGAGFGFIRFQSPTLPANTTIHIVDHLLSALNGLIDGPLERIPSIRLPREQVV
                     PARRILDESGLRSGTPYVFIHTGASSRQRVWPYAVDFARLLREKTGCTPVLHMGPIEK
                     ENAALEPHEVADMPILGPFTVLELAAALSCAAAYVGSDTGPSHLSAALGILTFTVFSG
                     NDEGRKNAVVWSPRGQRAHALYAQEGQEWPSPEQVVQALILSGVLP"
     gene            1304..2209
                     /locus_tag="PLT86_04495"
     CDS             1304..2209
                     /locus_tag="PLT86_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT35909.1"
                     /translation="MKHENRIVPYAPGHLEALLKVVNAHVAALPPQDGVIDRTALETI
                     VNRPEALWAHHTREHSIDAALVRMVAISDDEVVGLAVAWHEIGGEHAHLPLLVFEPRA
                     PGAAKALLGRIFDELRDRGCTSVATDERNPLGIGWFGVPDSWPHVLETMHQEGFSVAD
                     GWVILTGRTDSIPVEPPRPANEMITCVFVNNENMGEWRLEFFHDDKLVGECETWAVPP
                     HLASCPGFHDWITVEWVGVEEAFRRRGLAFWLLVKQMHYHLRAGRTRVALWTGPGNRP
                     ARALYYRLGFSDGPETFVLARSLAL"
     gene            2369..4939
                     /gene="clpB"
                     /locus_tag="PLT86_04500"
                     /pseudo
     CDS             2369..4939
                     /gene="clpB"
                     /locus_tag="PLT86_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
     gene            complement(5120..5422)
                     /locus_tag="PLT86_04505"
     CDS             complement(5120..5422)
                     /locus_tag="PLT86_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35910.1"
                     /translation="MKIDAEERVIRYLRHVLQGHPRSGQQYVDGLVAKGMTHAEVVSS
                     VLIPARARIADLRKAHYVNAIDAKNALSVTNQAIARLSPAQKAYRVTSPTAVSAAM"
     gene            complement(5618..6475)
                     /locus_tag="PLT86_04510"
     CDS             complement(5618..6475)
                     /locus_tag="PLT86_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOT35911.1"
                     /translation="MTTNTAGGSKLDDQALEAYLREVRDYVLLTREEEVQLARQIKRG
                     DQKALEKLTSSNLRFVISVAKKYQNRGMSLLDLISEGNVGLMEAAYRFDETKGFKFIS
                     YAVWWIKQAILKALSEQSRIVRVPLNRVGDAQKVDKRVSALCQYYGREPTIEEIASSA
                     EMSPEDVEKALDISRPHLSLDDPGHQEGGRTLLDTIPLEDEAFQESPFERRFGIGVEE
                     TLSELDEREAEIIRRYFGLDNQDPESLKDIGASFGITRERARQLKERALAQLRKTHKK
                     VNMESRRVV"
     gene            6845..7540
                     /gene="queC"
                     /locus_tag="PLT86_04515"
     CDS             6845..7540
                     /gene="queC"
                     /locus_tag="PLT86_04515"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006656598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOT35912.1"
                     /translation="MDKPRAIVLASGGMDSTVTVALANEQGVELALLHVNYGQLTERR
                     ELKAFHAVADHYRVPQSLRLVVDIGYLVQIGGSALTDATIPFPEPTFSDRRMIPSTYV
                     PQRNGNLLFIAAAWAEVIGAESIWAGLVEEDSSGYPDCRRSFCDAIERAIAEGNHDQH
                     SDPRIITPLIGLSKADIVRTGVRLAAPFEKTWSCYRREDTACGECDSCRLRLRGFAQA
                     GTRDPIGYANPGV"
     gene            complement(7549..8808)
                     /locus_tag="PLT86_04520"
     CDS             complement(7549..8808)
                     /locus_tag="PLT86_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT35913.1"
                     /translation="MDTSFQQGLPEGYPRSVRRPGDRQEFARRVGLIGQSPAIQQVLI
                     SIAQIAPTSTTVLITGETGTGKEIVARAIHELSPRRREPFVALNISALNEGTVESELF
                     GHEKGSFTDAVREKKGIFEAAGKGTVFLDEIGDISPALQVRLLRVLEEREFKRVGGIE
                     PLRAEARVITATNRDLAELVNAGTFRADLYWRLHVFEIHLPPLRERKEDIPLLVEHFV
                     ERFCSENNRAVPVISKEAMEYLLSYDWPGNVRELRTVVERVLVLSDVPIIRPENLPDM
                     VRDKVFANRLLPVASRGSGAEKMELGIIYRTLLELRADMADLKQILTAPGNGVASRSW
                     SSASLPPQDASSPASKPHVVDIEVSEDDTPSGETTPARTLTLRDLERDAIRQALVATK
                     GNRREAARSLGIGERTLYRKLREYNLS"
     gene            complement(8831..9517)
                     /locus_tag="PLT86_04525"
     CDS             complement(8831..9517)
                     /locus_tag="PLT86_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT35914.1"
                     /translation="MDAPGSYLRNVPFFAGLSESELAQIGTLVTQRVYPPENLIILAE
                     DEGEALFTILSGQVKVSILNEDGKEVILAVLGQGDFFGEMSLLDGKPRSANVIATEET
                     TVLILRRRDFLRLVERSPHMAIKVVAALTARLRKADRKIESLALMDVSGRVASVILQI
                     AEERGVPSQRGICIHNPPNQQAIANMAGTSRETVSRVMKRLKERGYVAMRGKDILITR
                     EADLRADYSL"
     gene            complement(9530..>11257)
                     /locus_tag="PLT86_04530"
     CDS             complement(9530..>11257)
                     /locus_tag="PLT86_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35915.1"
                     /translation="ALARTGSGSPDEALRQFDEALQIAPQGETLDRALFGSSLILMNQ
                     GKQDEARLRLERLIAERPLSPLVPESRFQLAGIMYEQRQYRAAARELEKMIRLRQSSR
                     DINEIRTRLVLAYEAVGEFESAARVSQEITKAANADAAAWGARHLIAAYMNMRRFAEA
                     EAALGEELALRPNAPDADSLTMVRGKILMERNQSGKAMAVLTGFERNYPRSKFVPEAW
                     RMLADVQFAQGSSEEALANYRKTIQARPSDKQARLGEVAALYRLGRTSEAQGRERALR
                     DLGPITEDDEVRLAIEQGHALARSNDYVGAINAFARVVERNPRSEWADDALLAQGRVA
                     ISSGRLEPAVQAFERLAQQYPDSPLRQEALFELANAYFQAEFYDQAADAYNRMLEQDT
                     TSQFAAQAMWNLILSYEQIQRLDSAVRTMRVFLSKFPDSPHVVRVWVKIGDDLNRLGE
                     FPAAVAAYRQALDNVAGTPEEPDARFGLGEAHFNAGQYREAVIEWLKLAYHSQSGSRW
                     AVTALFRAAKANEKLGQLEDAKMLYRKIIAMEGESDLGRAAALQLMSISGQNLDSGSE
                     SGSSPTDTR"
     assembly_gap    11258..11357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11358..13114)
                     /locus_tag="PLT86_04535"
     CDS             complement(<11358..13114)
                     /locus_tag="PLT86_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT35916.1"
                     /translation="MFRHHVRNPGLRLLAAILPALLTMGRVAAQQEDGEFAFAQHLMR
                     DRMYDLASQQLQQYISNYPNAARTPDAFLLLAEAHAARGAYAKAADTYQGFMIKYPQD
                     VRVRELWLKQAEMRARAGQFAEAARAYTELADSYPESEYADDALIGAASAFLKMNQPD
                     RAERAVNRLITRYPRSPLLSAARALLGRAKLDSGDPQGAQQAVQAVLAERQFSNPVGD
                     AVLVGVEAAIALNKPGEARALSDRLVAQAPGDDRTWLARRTLAEYLLQTGRRTRDAET
                     LNQAADLYKEIARRATTPAAMESALFDLALVRELQEAPALAISNWQDFLQQYPQSRRK
                     PRAMLGLARANLSGGDSRAGVFALEELLAAYPDSSESTEALGLLGEYYLSRNDPSSAV
                     VYFERQVARTAEGQARRSRLLNVARIRETRLGEPELARTLYGELAVGDDSIAAEGMFG
                     LARCERTLGNLDAAESAYFRVTQQFPAHALANAATDSLNLIKNYLRIDLQGAIIATMA
                     LEGDQVLVRGNADSLLREKPLRLAKIRIEYLKDYPGGIELLKAYLNQPGVSSPDIAEH
                     LLARCYFRLSTRAGLERNVA"
     gene            13605..15203
                     /gene="pckA"
                     /locus_tag="PLT86_04540"
     CDS             13605..15203
                     /gene="pckA"
                     /locus_tag="PLT86_04540"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOT35917.1"
                     /translation="MVGVVPEYQLDQHGFTNLGTVHWNLSTSLLYEEAIRRREGQLAH
                     LGPLVVKTGHHTGRSPNDKFVVQEESTESEIWWGKVNQPYEEKKFHGMYRRLLAYMEG
                     RDVFVQDCAVGADPRYRIPIRIITEDAWQNLFARNMFIQLPREELRTHVPQFTVIAAP
                     RFHAIPEVDGTRSEVFILVNFGKRLVIIGGTAYAGEIKKSVFTFLNYLLPRGDVLSMH
                     CSANVGPDGDTAIFFGLSGTGKTTLSADKGRNLIGDDEHGWSDHGIFNFEGGCYAKMI
                     RLSAEAEPDIYETTRKFGTILENVMMDANTRRLNLDDDGLTENTRGAYPLSHIPGAVV
                     PSVGDHPKNILMLTCDAFGVMPPIAALTADQAGYYFLSGYTAKVAGTEKDMGNEPQAT
                     FSTCFGAPFLALPPTRYADLLGKKVREHKTKCWLVNTGWSGGPFGVGSRMKIQTTRAL
                     IRAALSGKLDGVATKPDPFFGFLVPESCEGVPSDVLQPRNTWADKEAYDRQAKELAAR
                     FSENFKQYEGMAPREVTESGPKAG"
     gene            15306..17315
                     /locus_tag="PLT86_04545"
     CDS             15306..17315
                     /locus_tag="PLT86_04545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT35918.1"
                     /translation="MAASNKRRMEAEDLYRFRLISDCRISPDGSSVIYVLNRVERKTE
                     KKFANLWMVPVRGGNPVQFTQGDQIDSSPRWSPDGSRIAFLSNRGGAQNPHLHVIPVG
                     GGEAKKVVDLRGEFGDFVWANDNKTVYFAFRKTDHEVLEREKDETAKKLGVVSRKIER
                     VFFREDGHGFLPKERWHVWSLNTKTGKARQLTHGEIADEVYPSVSTDGERVAFLSNRS
                     HDPDLDPEAVDLFVMPARGGAERRIDTPIGAKSHPVFSPDGRWIAYFGTEGRGAWGQN
                     GKLWVVPSDGSAPPRNLTGEYDVDVPGWTINDLGGAVMMPPVWSPDSAAIYFQVARHG
                     CTNLCRIGIDGSNFETVVAETGVVGAFSFDAGFKRLAFFFANHVEPGQVKVRNLRTGS
                     ERQLTAENSALLQKIAWGTIEEVWFKGADGHNVQGWIAKPPDFDPRKRYPAILEIHGG
                     PHLQYGWFFMHEFHFLAANNYIVFFCNPRGSQGYGEAHARAIENDWGGADYRDLMKWT
                     DFVAQQPYVDSARLGVTGGSYGGFMTNWIIGHTHRFKAAVTQRSVSNMISMYGTSDFN
                     WSFEREMGGRPPWDSVENYWRQSPMKYLGNATTPTLVIHNENDLRCAIEQGEQVFTAL
                     KRLGVETAMIRFPDEFHGMSRNGRTDRRIERLKSILGWFDKYLKQ"
     gene            17336..18112
                     /locus_tag="PLT86_04550"
     CDS             17336..18112
                     /locus_tag="PLT86_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT35919.1"
                     /translation="MKIYLCTDMEGASGVTMRSEIEPGQERYEFGRRMLTRDVAAAVE
                     GLKAGGATRVVVLDGHGGGDNFLYDELPAGAEYICGRGGDPIPFLDDSFDAVGFVGQH
                     SMAGTPDAVWPHTQSWTDVLGLWANGREIGEIGQFAIFAGHYNLPVIFLSGDRAACAE
                     VRALCGEVQTVAVKQGCSPTRALCLSRAEADKAIYEAARESARGAIRTAPCRVEVPVE
                     IRVRFTKTSLADGCVQTGAERVDEVTVRRVVPSALDIYRM"
     gene            complement(18138..19424)
                     /locus_tag="PLT86_04555"
     CDS             complement(18138..19424)
                     /locus_tag="PLT86_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35920.1"
                     /translation="MVKAGFGLIEISPLVGTPVSGYLNARYASGVHDPLFARALALTD
                     GQTPALLISFDLIGLADADVAAIRKAVGERVPVDEQHIILFCTHTHTGPALVDSFETP
                     RSDEYARSIPEKTARAAETAWYDLAEAGVSFAQREVPGVAFQRRYLMKDGTVRTNPGK
                     MNPDIVGPQRRIRTPVTLIGFERDKTRLPIVVASFPCHADVVGGTEVSADYPGRVCDH
                     ITHEFPGHPETLFLRAPAGDINHLNVFDASPQSGYEYAKHMARAITERAVQAWRDRVS
                     TPGPLATACASVTLERRSVEQSELASARSVFAQSKMLSPLPIDAVWAREKLLLAEMPE
                     QIRVEVNGIRIGGVRILGVPGELFSELGDAIIARSPASNTLVADCCGGKLGYLPTREA
                     YSQNGYEERPARSSPCAPGSGEAVVEAALSLAAELA"
BASE COUNT         4134 a         5678 c         5654 g         3951 t
ORIGIN      
        1 ggcatcgcgc aatccgcgga gccctttcct gaggtggtcc agaacgtcga gaacatcccg
       61 tgatgacaac atggccgtca cgcccctatc cgttcagctt tcccgttacc gttgtccgaa
      121 ttctctgcaa caaaatgtac aaccggtttg ctcaggcgga gttaacggcg catgagtagc
      181 tcatgaaact ttgttcatgt acgccgatgt atgggttcat gggagcactc ccgacaggat
      241 cagtgcctgc accacctgtt ccggcgaagg ccattcctgc ccttcctgag catataatgc
      301 gtgcgcgcgt tgcccccggg gagaccatac tacggcgttc tttctcccct catcattgcc
      361 actgaatacc gtaaacgtca gaataccaag agcggccgaa agatggctgg ggcccgtgtc
      421 actgccgaca tatgccgccg cgcacgacaa cgccgctgcc agctcgagta ccgtgaaagg
      481 cccaaggatg ggcatatcag cgacttcgtg tggttcaagc gcggcgtttt ccttctcaat
      541 tggtcccatg tgcaacaccg gtgtgcaacc cgttttctcc cgcaacaatc tggcgaaatc
      601 cacggcatag ggccacactc gctgacggga ggatgcgccg gtgtggatga agacatatgg
      661 cgtaccggac cgaaggccgg attcatccag gattctcctt gccggtacta cttgttcccg
      721 gggcagacgt attgacggta ttcgctccag cggcccgtca atcagcccgt tcagagcact
      781 aagaaggtga tcgacaatat ggatggtcgt attcgcgggg agtgtgggcg actggaaacg
      841 aataaatccg aatccagcgc cgcgcgcggt ttcttcaagt tcatcatctt ccgaagtgaa
      901 cgcgatgagg gcgtccgcgc cttctaccag cgcgcgcatt ccggcggcgt caccggcaaa
      961 ccatttccgg gcccattccg aatctgccga taccaccgaa tcggtcagcc cgcatgtttg
     1021 cgcgaggagc cccacttctt ttgaagcgac gaaatggagg ggtacatggg gaaaagcaag
     1081 acgcaggttc tggagaaccg gaaaagtgac aatagtgtcc ccgagagctc cattcctgta
     1141 gacgacgatt cctctgacgc gctgaagggc gattggcttg gacattggat gctccgaagg
     1201 cttgattgcg ctggttacgc aggaacgtac atttcatccc tgtcaggccg agggagcccg
     1261 acctgtcagg caagaagaga actggaaatc ccgacaagac cgaatgaaac acgaaaaccg
     1321 catcgtcccg tatgcccccg gccacctcga agcccttctg aaggttgtca atgcccacgt
     1381 tgcagcattg ccaccacagg acggtgtgat tgacagaacc gcgctggaga ccatcgtgaa
     1441 ccgccccgag gcgctgtggg cgcaccatac tcgagagcac agcatcgatg ccgctttggt
     1501 tcgaatggtc gccatttctg acgatgaggt tgttggcctc gcggtggcct ggcatgagat
     1561 cggcggcgag cacgctcatc ttccgctcct ggtgtttgaa ccacgcgcgc cgggtgcggc
     1621 gaaggcgctc ctcggtcgga tttttgacga gttgagggac cgcgggtgca ccagtgtcgc
     1681 cacagacgaa cgcaatcctt tgggaatcgg atggtttggc gttcccgatt cctggccgca
     1741 cgtcctcgaa accatgcacc aggaggggtt ttctgttgct gatgggtggg tcattctgac
     1801 cgggaggacg gactccattc cggttgagcc accgcggccc gcgaacgaaa tgattacgtg
     1861 tgtgttcgtc aacaacgaaa acatgggaga atggcgcctt gaattcttcc acgatgacaa
     1921 gctcgttggc gagtgcgaaa cctgggccgt gcctccgcac ctcgcctcct gccccggctt
     1981 ccacgactgg atcaccgtgg aatgggttgg tgtggaggaa gcgttccggc gccgtgggct
     2041 cgcgttctgg ctactggtga aacagatgca ctatcacctg cgcgcaggcc gcacacgagt
     2101 agcactctgg accggccccg gcaaccgccc tgcgcgagcg ttgtattacc gcctgggatt
     2161 ctcggacggc ccggagacgt ttgttctggc tcgctccctc gctttgtaat gccaatgtgg
     2221 caccccgaac agcctcactg cctatctttc ctgtaagagt gcgccattat ggcataaatc
     2281 gctgcccatt tcctcgtggt atccttcttg caaaacacgt cgaaaaaaaa ccggacactc
     2341 acgaaggaga aataacgata tggcagcaat ggacgccaac cgaatgactc aaagcacgca
     2401 gaacgcgctg gctgctgctc agcagacggc gctgaatcgg aaccacaacc agttggaacc
     2461 cgagcacatc cttctggcgc tgcttgagat ggagcagaat ctcccgcagg agctactgag
     2521 gcgaagcggc gggaacgtcg agaccatcac gcgcgaggtg gtttcagccc tcgacaggct
     2581 accgcgtgtc caaggggaca acgtacaggt gtacgcttcg ccgaacgtcc agcgagtcct
     2641 ttccgctgcg tggaaacgcg cgcaggaact aaaggacgaa tacatttctg tcgagcacct
     2701 cctgctcgga atactcgacc agaaacacac gcaagcggcg aaaatcctcg aacgaaacgg
     2761 catcacccag gagaagctcc tccaggcgat gaaggaaatc cgcgggagcc agagggtcac
     2821 cgaccccgac gcagaatctc gctaccaggc gctggagaag ttcagccgtg acctcaccgc
     2881 cgcggcgcga gaaggcaaac ttgaccccgt gatcgggaga gatgaggaga ttcgtcgggt
     2941 catcaaggtc ctttcacgcc gccggaaaaa taaccccgtc ctgatcggtg atccgggagt
     3001 ggggaaaacg gctatcgttg aagggcttgc gcaacgcatt gtcgccgggg aggttcccga
     3061 ctccctccag gggcgtcggg tagtttcgct ggacctgggt tccatgatcg cgggatcgaa
     3121 attccgtggc gaattcgagg aacgcttcaa agcagtgctc caggaaattg aagcggcgga
     3181 aggtcagatc atcctcttca tcgacgagtt gcacacagtt gtgcgggcgg gagccgcgga
     3241 aggcgcgatg gatgcgggaa acatgctcaa acccgcactg gctcgtggcg tcctccgttg
     3301 cgtgggcgcg acaacgctcg acgagtaccg caagcacatc gagaaggacg cggcacttga
     3361 aagacggttt gccccggtct acgtcggtga accgtcggtg gaagagacga tttcgattct
     3421 gcggggactg cgcgaacggt acgaggtaca ccatggcgtg cgaatcaaag acgaagcgct
     3481 ggtggctgcc gcgcgcctgt ctcacaggta catatcagac cggttcctgc cggacaaggc
     3541 aatcgatctt gtggacgaag ccgcggcaga attgcggatt cagttggata gcatgccgcc
     3601 tgaactggaa gccattgaac agcgcattgt ccagctggaa atcgagcgct ctgccctcaa
     3661 gcgcgaggaa gacgccgagg agcgccttgc ccctgtcgaa aaggaactgg cgaatttgca
     3721 ggagaaacgt gcggcgctga aatctcactg gctcaaagag aaagagatcg tcaacgcgct
     3781 caaaaaactg aaggagcagg cggaagaggc tcggacagag ctcgaccgtg cacgcagaac
     3841 aggacaactt gagctggcgg gacgaatcca gtacagcgaa ctcccccgca tccaggcgga
     3901 aattgcacag gcgcaagacg agctggcacg tgtccaaaaa gaccagaaga tgctgcacga
     3961 agaggtcgaa gaggagcaca ttgcgggaat cgtcagcaag tggacgggaa tccccgtaag
     4021 ccggctggta gaaggcgaaa tggaaaagct cctccacatg gaagaacggc ttcacgaacg
     4081 tgtcgtgggg caggacgaag ctgtgacggc cgtcgcgaat gcggtgcgtc gtgcaagggc
     4141 gggattgagt gaccagaacc ggcccatcgg ttcgttcctg ttcctgggcc ccacgggagt
     4201 tggcaagaca gaactggcaa aggccctggc ggagttcctt ttcgacgacg aacgggcgat
     4261 ggttcggctg gacatgtctg agtacatgga acgccatagc gtgagccgcc ttgtgggagc
     4321 gcccccgggt tacgttgggt acgaggaagg gggccaactc acggaggccg tccgccggcg
     4381 gccgtatcag gtcgtgctcc ttgacgaaat cgaaaaggcg catcatgacg tcttcaacgc
     4441 cctgttgcag gtgctggacg acggtcggct cactgatggt cagggccgga cggttgattt
     4501 cagaaatacc gtcctcatca tgacgtccaa cgtcggaagc cagaacttcg cgttcgggga
     4561 gaccgccgat tacacgagaa tgcgcgcgca ggtgatcgag gctctgaaag ccgaattcag
     4621 gcctgagttc atcaaccgcg tggacgagac gatcgtgttc cacgggctcg aaaagaaaca
     4681 catccgggag atagtacgcc tccaaaccag actgctcgcc cacaggatct tcgagcaggc
     4741 aagggttacg ctgacactga cagacgccgc cagcgacctg ctgggggaac tcgggtacga
     4801 cccggcgtac ggtgcgcgcc cgttgaaacg cgtcatccag cgtcagctcg aaaacccgct
     4861 ggcgatcgcg cttctggaac ggcgcatcaa ggaaggcgat atggtaagcg tcgacgtcca
     4921 cgacggcgcg ctcgtgttcc gtaccacaaa gccggatgga tccgctgcac ccggcgccga
     4981 gtccgtgact tcgacggcgg aatcggtcga ggacgttgag gatgcggagt ttgaggagct
     5041 gaaataacct ctgagtggga aaagaaagag ccggcggggt tccgcggaac cctgccggct
     5101 ctttttgttg agcagggaat tacattgccg cagaaacagc agtcggtgac gtgactctgt
     5161 acgctttctg cgcaggcgaa agacgcgcga tcgcttgatt tgtcacggac aacgcgtttt
     5221 tcgcatcaat ggcgttgaca tagtgcgctt tccgcaggtc ggcgatccga gcacgggcgg
     5281 gaatcaggac gctggacaca acttcggcgt gcgtcatacc tttcgcgacc agtccgtcaa
     5341 cgtactgctg gccgcttcgc gggtgtccct gcagcacgtg ccgcagatac cgtatgactc
     5401 tctcctcagc atcgattttc atggtaatct cctcttcggt gcgctgttca attcgattgt
     5461 cgattttcat atcagtgtgt attgtggtac gcccggggaa catcccaaca aacaagcctt
     5521 tctgcacgaa acgcaagaag cctcccgcgc gttatggcac cggaggcttc aaattgcaaa
     5581 taccttgcca aacttggcag gcgtgattac gcgccgctca aaccacgcgt cggctttcca
     5641 tgttgacctt cttatgcgtc tttcgaagct gagcgagcgc acgttctttg agctgtcggg
     5701 cacgctcccg cgttattccg aaggaggccc cgatatcttt cagggactca gggtcctggt
     5761 tgtcgagccc gaaatagcgt cgtatgatct ccgcttcccg ttcatcaagt tcggataacg
     5821 tttcttccac cccgattccg aaccgccgct cgaacgggga ttcctgaaat gcttcatctt
     5881 ccagagggat cgtgtccagc agagtgcgtc ccccctcctg atgccccgga tcgtccaggg
     5941 acagatgggg ccgcgagata tcgagcgcct tttcgacgtc ctcgggtgac atttctgcag
     6001 agcttgcgat ctcttcgatc gtcggctctc tcccgtaata ctgacacagc gcacttactc
     6061 gtttgtcaac cttctgcgca tctccaaccc tgttcagcgg cactcggaca atccgcgact
     6121 gctccgaaag cgccttgagt attgcctgct tgatccacca caccgcgtac gagatgaact
     6181 tgaacccttt ggtttcgtca aatcggtacg ctgcttccat cagtccgacg ttgccctcgc
     6241 tgatgagatc gagaagcgac atacccctgt tctggtattt tttagcgaca ctgatcacga
     6301 aacgaagatt ggaactggta agcttctcca gcgccttctg gtccccacgc ttgatctggc
     6361 gcgcgagttg aacctcctcc tcacgcgtga gcagcacgta atcacgcacc tcccggaggt
     6421 acgcttccag ggcctgatca tcgagtttgg aaccgccggc ggtgttagtt gtcataggct
     6481 ccgctccgtt tgattgaagt tttttccatt tcgacaaaca gctacgtcag ggagttccac
     6541 gacgtggccg gagccgcaaa tgaatattct cgcaccactt gcagggtaaa gaaaacggtt
     6601 cccgcaagcg tcacgagaga tcgtgcacaa tatttggaat atactgcaat tcataatgtt
     6661 ccgctaacgc cggagacttc aatcctcaca ggcagcgcgg cgccaggcct gcacagtccg
     6721 gggcgaaccg gaattgtgtc accccccgga ttctctctgt gcattccatc gcattgcagc
     6781 cttggccaag gactccatgc aggccttcat ccggtgtcgt aaatttgaaa ttggaggtgg
     6841 ctgtatggat aaaccgcgtg ccatagtgct cgcatccggc gggatggaca gcacagttac
     6901 ggttgctctg gcaaacgagc agggtgtcga actcgcgctg ctccacgtga attatggtca
     6961 gctgaccgag aggcgcgaac ttaaggcatt ccatgcggtg gccgaccact atcgggtccc
     7021 gcaatcgttg cgtctcgtcg tcgatatcgg gtatctcgtc cagatcggcg ggtccgcact
     7081 gactgacgcg accatcccgt tccccgaacc cacgttttcc gaccggagaa tgattcccag
     7141 cacgtacgtt ccgcaacgaa acgggaatct gcttttcatc gccgctgcgt gggcagaagt
     7201 gatcggggcc gagtctatct gggcggggct ggtggaggag gattccagcg gttatccaga
     7261 ttgccgccgc agtttctgtg atgcgataga acgcgcgata gcagagggca accacgacca
     7321 gcattccgac cccagaatta tcacgcccct catcgggtta tccaaagccg atatcgtgcg
     7381 taccggtgta cgtctcgcag caccattcga gaaaacctgg tcgtgttacc ggcgtgagga
     7441 cacggcctgc ggggaatgcg acagttgccg gctgagattg cggggttttg cgcaggccgg
     7501 cacaagagat ccgataggtt acgctaatcc cggtgtttga caacggcttc aggacagatt
     7561 gtattcccgc agtttccggt acagagtacg ctctcctata cccagcgaac gagcggcttc
     7621 gcgccgattt ccttttgttg cgaccagtgc ctgccggatg gcgtcgcgtt caagatccct
     7681 cagtgtcagt gttcgggcag gtgttgtctc accggaagga gtatcgtctt cagaaacttc
     7741 gatatccacc acgtgcggtt ttgaagctgg cgacgaagcg tcctgtggag ggagtgaagc
     7801 gctggaccag ctcctgctcg ccacaccgtt ccccggcgct gtgaggattt gtttcaaatc
     7861 tgccatgtcg gcccgcagtt ccagcaacgt gcgatagatg atcccgagtt ccatcttctc
     7921 ggcgcccgat cctcgcgacg ctaccggaag gagccggttc gcaaacacct tgtcacgcac
     7981 catgtcaggc aggttttcgg gccgtatgat gggcacatca gagaggacga gcacgcgttc
     8041 cacgacagtg cggagttcac ggacattgcc gggccagtcg tacgagagga ggtattccat
     8101 ggcttcttta ctgatcaccg gaacagcccg gttattctcc gagcagaacc gttctacgaa
     8161 gtgctcaaca agaagcggaa tatcctcttt gcgctcccgc aggggaggca gatgaatctc
     8221 aaacacgtgg aggcgccagt acaggtccgc ccggaatgta cccgcgttga cgagttccgc
     8281 caggtcgcga ttggtagcag ttattacgcg agcctccgcg cgcaacggtt cgattccccc
     8341 gacccttttg aactcgcgtt cttccaagac gcggagcagt ctcacctgaa gcgccgggct
     8401 tatgtcaccg atctcgtcaa ggaaaaccgt acctttcccg gcagcttcga aaatgccttt
     8461 cttttcacga acagcgtcgg tgaaggatcc cttttcatgt ccaaacaact cgctttctac
     8521 ggtcccctcg ttcaacgcgc tgatgttgag cgccacgaag ggctcccgac ggcgcggact
     8581 caactcatgg attgcgcgcg caacaatctc cttccccgtt ccggtctctc ccgtgatgag
     8641 tacggtcgta gaggtcggtg caatctgcgc gatgcttatg agaacctgct gaatcgccgg
     8701 tgattgccct atcagaccaa cccgccgggc aaattcctgt cggtcccccg gcctcctgac
     8761 agaccgcggg tacccttctg gcaaaccctg ctggaacgat gtgtccatat acgttatccg
     8821 gttcccgaat tcagagtgag tagtcagctc gcagatctgc ttcacgcgtg atcagtatgt
     8881 ctttgccgcg catggcaaca taaccgcgct ccttgagccg tttcatgacg cgcgaaaccg
     8941 tttcccgaga tgtgccggcc atattcgcaa tcgcctgctg attcggcggg ttgtggatac
     9001 agatcccccg ttgtgaaggc acgccgcgtt cctccgcgat ctgaaggatc accgaggcca
     9061 ctcttccgct cacgtccatc aaggcgaggc tctctatctt tcgatccgct tttcgcaggc
     9121 gggcagtaag cgcggcgaca actttgatcg ccatgtgcgg agaacgctcc accaaccgca
     9181 ggaaatcccg ccgcctcaaa ataagcacgg tcgtctcttc agtggcgatc acattcgcag
     9241 aacgcggttt tccgtcgagc aatgacatct ccccgaaaaa atcgccctgc ccgagcaccg
     9301 ccaggatcac ttctttcccg tcctcattca ggatggaaac cttgacctgg cctgagagaa
     9361 tcgtgaacag cgcttcaccc tcgtcctctg ccaggatgat caggttctcc ggcggataaa
     9421 ccctctgcgt cacaagcgtt ccaatttgtg ccagctcgga ctctgacaac cccgcgaaaa
     9481 atggcacgtt cctcaggtag ctgccaggcg catccattgc ggtccactcc tatctcgtgt
     9541 ccgtcggtga cgacccggat tccgacccag aatcaagatt ctggcccgag atgctcatga
     9601 gctggagggc tgctgcacgt cccagatcac tctcgccttc catcgcgatg attttcctgt
     9661 acagcatttt ggcatcttcc aactgaccca gtttttcatt tgcctttgcc gcgcggaata
     9721 acgcggtaac cgcccatcgc gatccggact ggctgtgata tgccagcttg agccattcga
     9781 tcaccgcttc gcggtactga cccgcgttga agtgcgcttc tcccagaccg aaacgagcgt
     9841 ctggctcctc gggcgttccg gcaacgttgt ccagcgcctg cctgtacgcg gcgactgctg
     9901 ccgggaattc accgagacga ttcaaatcgt ccccgatttt cacccagacg cgcaccacgt
     9961 gcggactgtc agggaacttg ctgaggaaaa ccctcatcgt gcggacggcg gaatccagac
    10021 gctgaatctg ttcgtacgaa agaatcaggt tccacatcgc ctgggcggca aactggctcg
    10081 tagtatcctg ctcaagcatc cggttgtacg cgtcagcggc ctggtcatag aactccgcct
    10141 ggaaataggc attcgcaagt tcaaaaaggg cttcctgtct caacggactg tccggatact
    10201 gctgggcgag ccgttcgaac gcctggacgg ccggttccag tcgtccactg gaaatcgcaa
    10261 cgcgtccctg agcgagaagt gcgtcgtctg cccattcgct tcgcggattc ctctcaacca
    10321 cccgagcgaa tgcgttgatc gcgccgacat agtcgtttga ccgggcaagc gcgtggccct
    10381 gctcgatggc aaggcgaact tcgtcatcct cggtaattgg acccagatcg cggagcgcgc
    10441 gctccctccc ctgagcctcg ctcgtcctcc cgagcctgta cagtgcggca acttcgccca
    10501 gcctggcctg cttgtcggac ggtcgcgcct ggatcgtttt ccggtaattg gctaacgctt
    10561 cttcagacga tccttgcgcg aactggacat ccgcgagcat tctccacgcc tccggcacga
    10621 acttgcttct tggataattc cgttcaaaac ccgtcaaaac agccattgct ttgcccgact
    10681 gatttcgttc catcaggatt tttccgcgca ccatcgtgag gctgtcggca tccggagcgt
    10741 tcgggcgcag ggcgagttcc tcgccaaggg cggcttcggc ctccgcaaag cgacgcatgt
    10801 tcatgtaggc agcgatgagg tgcctcgctc cccacgcggc tgcgtccgca ttggcggctt
    10861 tcgtgatctc ctgagaaacc cgcgcggcag attcgaattc cccgaccgct tcatacgcca
    10921 gaaccaaacg ggtcctgatc tcgttaatat cacgcgatga ctgacgaaga cgaatcatct
    10981 tttcaagttc acgggcggcc gctcggtact ggcgctgctc atacatgatc ccggccaact
    11041 ggaatctgct ctcaggaacc agcgggctca acgggcgttc agcgatcaat cgctccagtc
    11101 gaagccgcgc ttcatcctgt ttcccctggt tcatcaggat caacgacgag ccgaacagag
    11161 cacgatccag agtttcaccc tgcggcgcga tctggagcgc ttcatcgaat tgccgcaacg
    11221 cttcgtcggg ggatccggag ccggttctcg cgagggcnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnngcc acattgcgtt ccaatcccgc gcgggtgctc agccggaaat
    11401 agcagcgcgc gagcaggtgt tcggcaatat caggggagga gacgccgggc tggttcaaat
    11461 acgccttcag gagttcgatc ccgcccggat agtctttcag atattcgatc cgaatcttgg
    11521 ccaatctcag gggcttctct cgaagaagag aatccgcgtt ccctcgcaca agaacctgat
    11581 cgccttccag cgccatcgta gcgatgatgg caccttgaag atcgatacgc aggtaattct
    11641 tgatgaggtt caggctatcc gtcgccgcat tcgcgagggc gtgagccgga aactgctggg
    11701 ttacgcggaa ataggcgctt tctgcggcgt caaggttgcc gagagtccgt tcacacctcg
    11761 cgagaccgaa catcccctcc gccgctatgc tgtcatctcc aacagcgagc tcgccgtaca
    11821 atgtgcgtgc aagctcgggc tcgccgaggc gagtctcacg tattcgcgca acgttcagga
    11881 gtctcgatcg tctggcctgg ccttcggcgg tacgggcaac ttgtcgctcg aagtacacca
    11941 cggcgcttga tggatcgttc cgggaaaggt aatactcccc gagcagcccc agcgcctcgg
    12001 tgctctcgct ggaatccggg taggccgcga gaagctcttc aagagcgaac acgccggcgc
    12061 gtgaatcacc tccggagaga ttcgctcggg ccaggccgag catcgcgcgc ggcttccgcc
    12121 tgctttgagg gtattgttgc agaaaatcct gccagttgct gatcgcgagc gccggcgctt
    12181 cctgcagttc cctgaccaga gccagatcga agagcgctga ttccatagca gcaggtgtcg
    12241 ttgcacgacg cgcgatttcc ttgtagaggt ccgccgcctg attgagggtt tcggcatccc
    12301 gtgtcctccg ccccgtctgg aggaggtatt ctgccagtgt gcgtcgagcc agccacgttc
    12361 gatcgtctcc gggcgcctgg gcaaccaggc gatcactcaa tgcacgggct tcgccgggtt
    12421 tgttgagggc aattgctgct tccacgccca ccagaaccgc gtcgcccaca ggattggaga
    12481 actggcgctc agcgagaacc gcctgcacag cctgttgagc gccctgcggg tctccagaat
    12541 ccagtttggc gcgaccaagc aacgctcgag cggcggacag caaaggggaa cgaggatacc
    12601 gggtaatcaa acgattgaca gcgcgttccg ctctgtccgg ttgattcatt ttgagaaagg
    12661 cagacgctgc tccaatgagt gcgtcgtcgg catattcgct ttcagggtac gaatcggcaa
    12721 gctctgtgta tgctctcgcc gcttcggcga actggcctgc acgtgcgcgc atctcggcct
    12781 gcttcagcca aagctcgcga acacgaacat cctgcgggta tttgatcatg aacccctgat
    12841 aggtgtccgc cgccttcgca tacgcacctc gcgccgcatg agcctccgcg aggagcagaa
    12901 acgcatccgg agttcgggcc gcgttcgggt agttgctgat gtattgttgc agttgctggc
    12961 tggcaagatc atacatccgg tcgcgcatga ggtgctgcgc aaacgcgaat tcgccgtcct
    13021 cctgttgtgc cgcgactctg cccatggtta gcagcgccgg gaggatggca gcaagtagtc
    13081 gcaaacccgg gttgcgaaca tgatgacgaa acatcgtacc tccttcgaac gttctcacgg
    13141 gcggccatta tggcagataa tgtaccctgc gtggcgctaa aatccaagtt atcatgcgtt
    13201 tgcatcaacg ccattgtgcc atgcgtacac tgagtgcaac caggcataac ggaaggcaag
    13261 aacaggagtt ccaactgtgc atacgcaggg gacagcattt cagatcccac tctgaggccg
    13321 gaaatctgtt cgagagcaac gccgatattc cacggaagga gagagacaca cctgatttcg
    13381 cgggtgcccg atcgcccgcg ggagaacagg caagcgcgtg tcagaatggg gctatgagtt
    13441 ggccacgccc tgcgccttcg gtatattttc cccgctgttt ttccagacag gggcgccggg
    13501 atcgtcgcat gcaggcgaac gcgatgcatc ggtccggttg caccagaaaa gcagcgcagc
    13561 acagaatcac gccgaccgcg ccatagggaa aaggagaacg gaaaatggta ggggttgttc
    13621 ccgagtatca actggatcag cacggtttca cgaaccttgg gacggtccac tggaatcttt
    13681 cgacgtcatt gctttacgag gaagccatca gacggagaga aggacaactc gcccatctcg
    13741 ggcccctggt ggtaaaaacg ggacaccata ccgggcgctc accgaacgac aagttcgttg
    13801 tgcaagagga atccacagaa tcagaaatct ggtggggcaa ggtcaatcaa ccctacgaag
    13861 agaagaaatt tcacggaatg taccgccgtc tccttgcata catggaaggt cgggacgtct
    13921 tcgtccagga ttgcgcggtt ggggccgatc ctcgttaccg gattccaatt cgcattatta
    13981 cggaggacgc gtggcagaac ctttttgcac gcaacatgtt catccagctt cctcgcgagg
    14041 aattgcgcac ccatgtacca cagtttacgg tcatcgcagc tccgcgtttt cacgcgatac
    14101 cggaagtcga cggaacgcgc tctgaagtgt tcattctcgt gaacttcgga aaacgcctcg
    14161 ttattatcgg cggaaccgcc tatgccgggg agatcaagaa atcggtattc acgttcctga
    14221 attacctgct gcctcgcggc gacgtgttat ctatgcattg ttcagccaac gtcggtccgg
    14281 acggagacac tgccatcttt ttcggtcttt ccggtaccgg aaaaaccaca ctttcggctg
    14341 acaaaggtcg caacctgatc ggggacgacg agcacgggtg gagcgaccac ggcattttca
    14401 attttgaagg cggctgctac gcaaaaatga tccgcctttc ggccgaggcg gaacctgaca
    14461 tctacgaaac aacacgaaag tttggcacaa ttctcgaaaa cgtcatgatg gatgcaaaca
    14521 cccgtcgtct caatctggat gacgatgggc ttactgagaa cacgcgcggt gcgtacccct
    14581 tgagtcacat tccgggcgcc gttgtcccaa gtgtcggtga tcatccgaaa aacatcctga
    14641 tgctgacgtg cgacgccttc ggcgtaatgc ctcctattgc cgcgttgacc gcggatcagg
    14701 cagggtacta cttcctttcg ggatacactg ccaaggttgc cggaacggaa aaggatatgg
    14761 gaaacgagcc ccaggcaacc ttcagcacct gctttggcgc tcctttcctc gcgctgccgc
    14821 cgacacggta tgcggacctg ctcggaaaaa aggtccgcga acacaagacc aaatgctggc
    14881 ttgtgaacac ggggtggagc ggcggaccgt tcggcgtagg gtcacgaatg aagatccaga
    14941 ccacccgtgc gctgatccgc gccgcccttt caggcaagct ggacggcgtc gcgacgaaac
    15001 cggacccgtt tttcgggttc cttgtccctg agtcatgcga aggcgtcccc tcggatgtgt
    15061 tgcaaccgcg caatacgtgg gccgacaaag aagcgtatga ccgacaggcg aaagaactcg
    15121 cggctcgctt ctcggagaac ttcaaacaat acgaaggaat ggctccgcga gaagtcacag
    15181 aatcgggacc caaggcgggt tgacactttc cctgactggg caacggaggg gttggtaagc
    15241 gagagcgcca gcccctgtcg tttttcgttt cgtcggggtt cccgctggac cgcccgggag
    15301 gaatcatggc tgcaagcaac aaacgcagaa tggaagcgga agatttgtac cgcttcaggc
    15361 tcatcagtga ctgccggatc agccccgacg gcagttccgt tatctacgtt ctcaaccggg
    15421 tcgagcgcaa aacggaaaag aagttcgcga atctctggat ggtcccggtc cgtggcggca
    15481 accccgtgca gttcactcag ggcgaccaga tcgattccag tccccggtgg tccccggacg
    15541 gatcgcgaat tgcgttcctg tcaaaccgcg gtggtgcgca gaatccgcac ttgcatgtga
    15601 tccccgtcgg gggcggggaa gcgaaaaagg tcgtggatct tcgcggcgag ttcggcgact
    15661 ttgtctgggc aaacgataac aagacggtgt atttcgcatt ccgcaaaacc gaccacgaag
    15721 tcctcgaacg cgagaaagat gagacagcga aaaagctggg cgtcgtctca aggaagatcg
    15781 aacgcgtgtt cttccgcgaa gatggccacg gatttcttcc caaagaacgc tggcatgtgt
    15841 ggtcgctgaa cacaaagacc gggaaagccc gccagctgac gcatggagaa atcgctgacg
    15901 aggtctatcc cagcgtctcg acggacggag aacgggtggc gttcctttcc aaccggagcc
    15961 atgaccccga cctcgatccg gaggcagtgg acctgttcgt catgccggca cgaggcggcg
    16021 ctgaacggag aatcgatacg cccatcgggg cgaaaagcca tccggtgttc tcgcctgacg
    16081 gacgttggat cgcgtatttc gggaccgagg gcaggggcgc ctggggacag aacggcaagt
    16141 tgtgggtggt cccaagtgat ggatcggctc cacccaggaa cctcacagga gagtatgacg
    16201 tggacgtccc ggggtggaca ataaacgacc tcggcggcgc ggtgatgatg cctcctgtat
    16261 ggtctcctga ttcggcggcg atctactttc aggtcgcccg ccacgggtgc acgaacctct
    16321 gccgtatcgg catcgacggg agcaacttcg aaaccgtcgt tgcggaaacg ggtgtggtag
    16381 gtgctttttc ttttgatgcg gggttcaagc gcctggcgtt cttctttgcc aatcacgttg
    16441 agccgggtca ggtgaaagtc cggaaccttc gcacagggtc ggagcgccag ctcacggcgg
    16501 agaattcagc acttctccag aaaatcgcgt ggggaaccat tgaggaagta tggttcaaag
    16561 gcgccgatgg ccataacgta cagggttgga tcgccaaacc gccggatttc gacccgagaa
    16621 agagataccc ggccattctc gagattcacg gcggtccgca tctgcagtac ggctggttct
    16681 tcatgcacga gttccatttt ctcgcggcca acaactacat cgtctttttc tgtaatccac
    16741 gcggcagcca gggctacggc gaagcgcatg cccgtgcaat cgagaatgac tggggtggtg
    16801 ccgattaccg ggacctcatg aaatggacgg atttcgttgc acagcagcct tacgttgact
    16861 ccgcaaggct cggagtaacc ggcggcagct acggtggttt catgacgaac tggatcatcg
    16921 gtcacacaca ccgattcaaa gccgcggtta cccagcgaag cgtaagcaac atgatcagca
    16981 tgtatggaac gagcgatttc aactggagct tcgagaggga aatgggtggt cgccccccgt
    17041 gggatagtgt ggagaattac tggaggcagt cacccatgaa gtacctcggg aacgccacaa
    17101 cgccgacgct ggtcatccac aacgaaaatg acctgaggtg cgccatcgaa cagggcgagc
    17161 aggtgttcac ggccctcaag cgactgggcg tcgaaactgc catgatccgt tttcctgatg
    17221 agttccatgg aatgagccgc aatgggcgaa cggatcgccg aatcgagcga ttgaaaagca
    17281 ttcttggctg gttcgacaaa tacctgaagc aatgacgttt tgagggagaa aaggcgtgaa
    17341 gatatacctt tgcacagaca tggaaggcgc gtccggcgtt actatgcggt ccgagattga
    17401 accgggccag gaaaggtacg agttcggaag gcgcatgttg actcgcgacg tggcggcggc
    17461 ggtggaaggt ttgaaagcgg gcggagcaac gcgagtagtt gtgctggatg gacacggcgg
    17521 cggagacaac ttcctttacg acgaattgcc cgcgggagcc gaatacatct gtggccgcgg
    17581 cggcgatcca atcccgttcc tggacgactc attcgacgcc gtagggtttg tagggcagca
    17641 ttccatggcg ggtacgcccg acgcagtctg gccccacaca cagtcgtgga cggacgtact
    17701 gggtctttgg gcaaacgggc gtgagattgg cgagatcggg caattcgcga tttttgcggg
    17761 gcattacaac ctgccggtca tcttcctcag tggcgatcga gcggcgtgtg ccgaggtacg
    17821 agcattgtgc ggggaagtgc agaccgtcgc cgtgaagcag gggtgctctc cgacgcgtgc
    17881 gctctgtctg tcacgtgctg aagcagacaa agcgatttac gaagctgctc gggaatccgc
    17941 ccgcggcgca atccgcacgg caccttgcag agtggaggtt cccgtggaaa tccgggttcg
    18001 cttcacaaaa acctctctgg cggacggatg cgtccaaaca ggggctgaac gcgttgacga
    18061 agtgaccgtt cgacgtgtgg taccctccgc gctggacatt taccgcatgt gagtttcagc
    18121 aacaaaacac cgtcggttca cgcgagttcg gccgcaagac tcagagcagc ctcgacgacc
    18181 gcctcccccg aacccggtgc gcaagggctc gatcgtgcgg gacgttcctc gtacccgttc
    18241 tgactgtacg cctctctcgt cgggaggtac ccgagttttc ccccgcagca gtctgccacg
    18301 agtgtgtttg aggcaggaga gcgagcgatg atcgcgtccc ccagttccga aaacaactct
    18361 cccggcacgc ccagtattct caccccgccg attcggattc cgttcacctc tacgcggatt
    18421 tgttctggca tttccgcaag aagaagcttc tcgcgcgccc agactgcatc gatcggcaac
    18481 ggcgaaagca tcttggattg tgcaaacacg ctgcgggcag aagcgagttc gctttgttcc
    18541 acggaacggc gttccagggt gacggaagcg cacgcggttg ccagagggcc gggagttgaa
    18601 acacggtcgc gccatgcctg aacggcccgc tcggtaatcg ctctcgccat gtgttttgca
    18661 tactcgtacc cgctctgagg agacgcgtcg aacacgttca ggtggttgat atcccctgcg
    18721 ggagcacgca ggaacagcgt ctccggatgc cccgggaatt cgtgcgtaat gtgatcacac
    18781 acccgcccgg gataatcggc ggacacttcc gtccccccga ccacgtctgc gtggcacgga
    18841 aaactggcaa ccacgatcgg cagtcgtgtt ttgtcacgtt caaagcctat cagggttacc
    18901 ggtgtccgaa tccgacgctg gggacctacg atgtcggggt tcatcttccc ggggttcgtc
    18961 cgcacggtcc catctttcat gaggtatcgg cgctgaaacg caactccggg cacctctctc
    19021 tgagcgaagg acaccccggc ttcggcaagg tcataccatg cagtctccgc cgcccttgcg
    19081 gttttttccg ggatagagcg cgcgtactcg tcactcctgg gtgtctcaaa agagtccacc
    19141 agtgcgggac ctgtgtgagt atgcgtgcaa aacaggatga tatgttgctc gtcaacgggg
    19201 acacgctccc caacggcctt tcgtatggca gcgacgtctg catccgcaag cccgataaga
    19261 tcaaacgaga ttaagagtgc tggtgtctga ccgtcggtca gggccagtgc gcgtgcgaag
    19321 agaggatcgt gcacgccgct cgcgtagcgc gcgttcagat accccgatac aggcgtgccg
    19381 acgagcggac tgatttcgat aaggccgaac ccggctttga ccattccaga aacccctcca
    19441 atacgagaat ttggcgtcct tcagttccag cgcgaagcca tggcaccggc ccagggccag
    19501 gtcacgcagc acaccag
//
LOCUS       DAOSOO010000039        19388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2822_length_19388_cov_5.344178, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000039 DAOSOO010000000
VERSION     DAOSOO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19388
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_19388_cov_5.344178"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLT86_04560"
     CDS             complement(<1..130)
                     /locus_tag="PLT86_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT35921.1"
                     /translation="MNNVLLAFGLTVFAGMATGIGSIIAFTASKTNYRFLSVATGFS"
     gene            complement(191..1531)
                     /locus_tag="PLT86_04565"
     CDS             complement(191..1531)
                     /locus_tag="PLT86_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35922.1"
                     /translation="MTPSPITWVPPATFDANAPDLGLPPFPAAAHTVLYDPFPCAANV
                     DEGGDGRYESLRHGTYSHHPAIVLKDRTFIVYWTQHSKDENGPGQRMLAYAGQFDEAF
                     EEIDWSRDGALVEIIPPPIPVRRRWWNHDPHIIYPYALGGLQLINGRLYTSGLIVACH
                     GYTNDVQYHGFQTKPLPASAWSDDLDRERGFIYDLWLDLGLRFVQRWRIQDGVLAADS
                     PMYAQAPLTRRFEVCDGRWKDIAPLLPPYSEALPFDAAPTEMRDDILNGVPVQFARHA
                     KYAPGTSRLTEDGTNGLAHETEFRRRDGKWVVLRDNLERRGHYWAALKKREEDCYPPA
                     VETNLYGGAMPVAGEIPSGRVWVICNSFDDYYNAADKSRKVMFITLSDDGITFDRTYE
                     LLHVDREPDGGMYKFGGPQYYKALTVGKNVWVVYSITKEKVGVTKLPVDALERA"
     gene            1787..3040
                     /gene="ahcY"
                     /locus_tag="PLT86_04570"
     CDS             1787..3040
                     /gene="ahcY"
                     /locus_tag="PLT86_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT35923.1"
                     /translation="MASQVKNPALAEQGRRNIEWAERQMGALLKVQERFAKEKPLVGV
                     RVGMALHVTKETAVLVKTLVAGGAEVSITGCNPLSTQDDVAAALALEPHVEVFAHKGE
                     TREEYYDFLNRVIAFKPHVTIDDGCDLVSEIHTKHQGLIPDVICGCEETTTGVIRLAA
                     MEKDCALKYPMIAVNDSDTKHLMDNYYGTGQSTLDGILRASNILISGKVIVVVGYGNC
                     GKGVASRARGLGARVIVTEVNPFRALQATMDGFAVMPMAEAVREGEVFVTVTGNKNVV
                     STEHIRAMKDGAILANSGHFDNEVDVAGLTAMAKAKRTVRPMFDEYDLGGKVVFLAGE
                     GRLVNLAAAEGHPSTVMAMSFCNQAMAVEWGVKNRGKLEAKVYRLPPELDLEVSRLQL
                     EAMGIRIDKLTLEQEAYLNSWQEGT"
     gene            complement(3152..3244)
                     /locus_tag="PLT86_04575"
     tRNA            complement(3152..3244)
                     /locus_tag="PLT86_04575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3208..3210),aa:Ser,seq:gga)
     gene            complement(3282..3370)
                     /locus_tag="PLT86_04580"
     tRNA            complement(3282..3370)
                     /locus_tag="PLT86_04580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3334..3336),aa:Ser,seq:cga)
     gene            complement(3377..3838)
                     /gene="tadA"
                     /locus_tag="PLT86_04585"
     CDS             complement(3377..3838)
                     /gene="tadA"
                     /locus_tag="PLT86_04585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT35924.1"
                     /translation="MDRIPDHETWMRLALAEARAALHEQEVPVGAVVVRDGIVIGRGH
                     NTVETGESALNHAEMLALREATQAVGAWRLNDAVLYVTLEPCMMCAGALMLSRIGGLV
                     FGARDPKFGACGSRIDALAPDMGWNHRVNIISGVLADESAALLREFFSSRR"
     gene            complement(3895..3968)
                     /locus_tag="PLT86_04590"
     tRNA            complement(3895..3968)
                     /locus_tag="PLT86_04590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3932..3934),aa:Arg,seq:acg)
     gene            complement(4050..5369)
                     /locus_tag="PLT86_04595"
     CDS             complement(4050..5369)
                     /locus_tag="PLT86_04595"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOT35925.1"
                     /translation="MAEDTLTVTDNRTGKVYVIPIRYGTYPEYGASISAMDLRQIKVS
                     EDDFGLLSFDPALTNTETTASAITFIDGERGILRYRGYPIEQLAEKSTFLEVAYLLIH
                     GKLPTFAEFADWTNKIVKNMLIHESIKKFLDGFHSNAHAMGVFVGTLGAKSTFYPAAK
                     HVLDPKQRMRETINIIASVPTIAAYTYRHSCGLPYVYPDPSLSYIYNFLNMLFRMQPG
                     KEEIYQNPVIERALDVLFILHADHEQNCSTTTMRTVGSALSDPYSAISAAAGALYGQL
                     HGGANQEALEMLAQIGSTENIPAFIKRVKNHETRLMGFGHRVYKNYDPRAKIIKKMAD
                     QVLEVTGPDPLIDVARELECIALSDEYFIKRKLYPNVDFYSGIIYKALGFPVDMFPVL
                     FALARTAGWLSHWLELMDNEEERKIVRPRQQYVGEDEREYVPMDDRT"
     gene            complement(5430..6254)
                     /locus_tag="PLT86_04600"
     CDS             complement(5430..6254)
                     /locus_tag="PLT86_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT35926.1"
                     /translation="MKKLELGLMVGFASDPNESFRQVAELGIPTCQVTGTAEAALAGK
                     YSDPEKVRNAADKAGVRISSVFLLWEGQQFDNVNGPSTMGLVAPRFRDERLRSGKLFS
                     DWVARMGIDSITCHIGFIPDDERDPVYETFVDAMRDLALHCKRNGQKFCFETGQELPS
                     TLKRTIRDVGTGNLFVNLDPANLILYGKANPLDAEEIFGEYVRGLHAKDGVWPNRDEA
                     LGHETPLGEGAVRFDLLIRRLKAKGFEGPLTIEREIHGPQQRVDILRAIKILEPML"
     gene            complement(6296..6694)
                     /gene="queF"
                     /locus_tag="PLT86_04605"
     CDS             complement(6296..6694)
                     /gene="queF"
                     /locus_tag="PLT86_04605"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOT35927.1"
                     /translation="MTTTTGYDENHAKSQGETPAIDVWPNQYRDRSYTIRITIPEFTS
                     MCPKTGQPDFATITIVYEPDALCVELKSLKLYMQAYRNMGIFFENVANKILNEFVAAC
                     KPRRAVVVADFNPRGGIRTSVRVSYSNKSN"
     gene            complement(6710..7585)
                     /locus_tag="PLT86_04610"
     CDS             complement(6710..7585)
                     /locus_tag="PLT86_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOT35928.1"
                     /translation="MRVILDCDTKNEIDDQFAITYAFAKGVRIEAIVNVQNTHSNGPE
                     SVTIYQEEAAAILAALGRPEVPNLRGVERPLDNSVSPGSAEGVDFIIDRALTEDPEPL
                     FVIGTGPATDLASALLRAPEIASKARFIWLGGFRDEEEFRRRGSEWNALGDPWAVKAL
                     FESRAAMLSTLFHVPAWGVTEKLVWRTEWLVGQLREMAKPIHTLLADRTVEWTTRSGR
                     SLSDNPPKEFWDIGAVDVAIHPERHSLRFMPAHGLGENAEWVFPASGTHVIGIDVDLE
                     TDAILSDALCAFAGL"
     gene            complement(7600..8250)
                     /locus_tag="PLT86_04615"
     CDS             complement(7600..8250)
                     /locus_tag="PLT86_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT35929.1"
                     /translation="MSDELLDVCDDNGCHVAEATRSEVHEQGLWHKTVHVWVFAPGLG
                     DGHVLFQQRSAGKDIAPGMIDVAVGGHLNKGENPLAGAVRELGEELGLHVPASRLRFL
                     GVRQSAHRFGQFVDREIQTVYGIVDNRGLEDYSVAAEEIAAIVHVPAGDGLELFLGRV
                     NRLVAPVFYSTNPGNAPATGTRVLTNADFLPSVDGYFARVCLAAQHVRRGDADVLL"
     gene            complement(8240..9364)
                     /gene="aroB"
                     /locus_tag="PLT86_04620"
     CDS             complement(8240..9364)
                     /gene="aroB"
                     /locus_tag="PLT86_04620"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT35930.1"
                     /translation="MARTETVEVPLGSRTYPVVIGSSMLCTLGKSVRRVTAGRLVGVV
                     SDKTVWSLYGDQCSASLEAAGFRVVPKIIEPGEEHKTLHTVAGILDHFLAAGFDRESL
                     VIGFGGGVAGDVAGFVAAICLRGLPFVQVPTTVVAQVDASVGGKTGVDHPLGKNLIGA
                     FHQPSLVFVDTALLASLSERDLRAGLVEVLKHAVIRDPGLFDLLEASLDAFVAGTASA
                     DSWIDLVAANCRIKASVVGQDEKESGLRAILNYGHTAGHAIEKLGAYQRYRHGEAVGF
                     GMMVAGRMACERGLWRSDELFRQRRILRRLLRIPPPTDMAPRDIWNAMKGDKKSLGGV
                     LRFVMPRGIGDATVVSDVTEDEFAAAWAVTVNREEEPHVG"
     gene            complement(9459..9531)
                     /locus_tag="PLT86_04625"
     tRNA            complement(9459..9531)
                     /locus_tag="PLT86_04625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9496..9498),aa:Phe,seq:gaa)
     assembly_gap    9547..9646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9648..10520)
                     /locus_tag="PLT86_04630"
     CDS             complement(9648..10520)
                     /locus_tag="PLT86_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOT35931.1"
                     /translation="MLEPLVAGTLHGNFERLTGRGFLEDGIIQADEGSVSKDISSILE
                     GWDFRPDRPAVRRIVGDDGRPRLQIRLDLGLLQMEISGRPDGKRPHGFTTALEYYMHL
                     AEEAPGGEHEGGEFRLDEEDCVRLQQESMQFYHRRISWLELGEYRAAEEDADHNLKII
                     DFVVKYAENDQLREMFTQWKPFILVHKFLAGARQAWEAKDFDGAVERINRGIEDIAEI
                     YREQGREDAIENSGEISYLKKWLEDISQTRPLTLEQRVERELREAIASEHFERAAVLR
                     DRLQALRQSARTSE"
     gene            complement(10641..11030)
                     /locus_tag="PLT86_04635"
     CDS             complement(10641..11030)
                     /locus_tag="PLT86_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35932.1"
                     /translation="MARSTQILRRLNQAPPVQETSYNQPAHRPETERTEDETDHDRFR
                     RKQQRLAAQDTSSSLPIKVVEKQATKPQPGTRFEGPLEVLAAKYKDPREVMRHMTVGQ
                     YMRYELRGGQIWENGKLAYDGTQFLTE"
     gene            complement(11172..11248)
                     /locus_tag="PLT86_04640"
     tRNA            complement(11172..11248)
                     /locus_tag="PLT86_04640"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11212..11214),aa:His,seq:gtg)
     gene            complement(11366..12169)
                     /locus_tag="PLT86_04645"
     CDS             complement(11366..12169)
                     /locus_tag="PLT86_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT35933.1"
                     /translation="MLVWQNMHTHTHRSNCGGRDSTIANTVAEAESVGLTLIGVCDHI
                     DEPGSGREPLIVENMKERDELDTSVRVLIGSEVSMNAPDLPAVEPEIMATWDYVIISA
                     NHFHLPCVSQPDERTEEGYADHFLTMAEGAVRYGASIIPHPFSYVGVRTMEDGREIDW
                     PRLMAAYDMKRVRRLFAEAAAKGTAFEINPGRLGIMPEFFRKIIRMGRDEGMKFSLGS
                     DGHHPGQMHYGGPEKIAEIESQLRNLGVLETDICSNYAPRACTARAGTV"
     gene            12273..13460
                     /locus_tag="PLT86_04650"
     CDS             12273..13460
                     /locus_tag="PLT86_04650"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT35934.1"
                     /translation="MTVSGALLIDHFVRQWAELHEREWELTPLHTLTEKARHALGTDG
                     CRAALALLSVWNQHQPKFPTWRHLAADRQSAEQASGERAARWRAGRLSGCTRILDLCC
                     GIGGDAIALSSHCEHLVAIDRDFQRALCARFNVSSFGHGDRTDVVCSDVGAVLPAAEA
                     AVLDPDRRPGGRRVIRPELYEPPACLWAQIRKSVRTLAVKVAPGIPFDAIPADAVPEF
                     VEDRGECREAVLWFGERKPHARTAVILGAGSTVTASCIDASAPESFQKGEVGRFVYDP
                     SPAVVRAQLVTHLAAKLSAWRLDDRIAYLSSDSLVETPFARGFEVLSVIPFSLKRLRA
                     HLRDKRVGAVEIRTRRFPVNPDELRPMLDLRGPESATVILTRVRGNALAIVCRQGNSG
                     DLI"
     gene            13544..18262
                     /locus_tag="PLT86_04655"
     CDS             13544..18262
                     /locus_tag="PLT86_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOT35935.1"
                     /translation="MNAEYAWVEGRPVVRVDSLGINAFLTDWLVLDPFPFELPGGLNR
                     ELWIGRADAVDADPTGVERTVRPREGDKQRNDAVAGGFSVWQRLIGSTVHDLKSRYPG
                     FSSALVYAATYLHADTDTILAFCIDDWEIYRSCTAQLLLDGVEVCFGPSPAVVRIPAG
                     THCLIAKIGGGSGSGFAWKLSVRAGRVETTPAGWGVSLARLPGFWRGEECAPEVEVDV
                     VLVNPTGSPLEAGKIAANLCGQTQGAPSENHFLDPWQAKAIRISAPLGACSPESTTTV
                     ELAFYGRTVAFPVTVPRLLPPCTIHVLEGFHCDPVWVSDQRHYNLISLENVKMQVDAC
                     LVDPGYRAFVHELDYLKSFVDEYPDYRAKVFELVASGQLIVGSSYSEPNENNISGEAI
                     IRNIIYGQGYHRHFLGGDPRVYHAWDVFGHIPQLSQILARSGHRAALWSKSILGFPPL
                     FRHMSLDGTVLPHIRTHYTWYSNSLDNLRTISEPVVREKQSFGWHRHLMVEASDFTPP
                     SAWMIGRTAEMAASYPRILMTGPEEFLDGLTADGARLPLTSRNPSQHHVGTFHTRVEL
                     KLANRLAENMLFAAEIWSTFSGILGAEYPDHLIDKAWRQLLFGQHHDAITGTPCDVSY
                     LDLMAGYREALRLARGVLEDATTYIADAVTVSRDGTSVVAFNPLAWERDGVITIQKPE
                     GFEGIEVRNQDGTSLPCFANETTVEILAKKIPSVGYSTLELLPAPARSEPTVTRCYVL
                     ENEYWRMELDPARGGGIVSLFDKEQGKEIVDSALGVGNDLLALGNGPNPWEFHTTGER
                     VAASDSPAVVEVHTTPTSQTAVVSGRLGEICSYRRTLTLRPGSRTIEASVVLSGYRAN
                     GHQFVATFPTALQGALPIFEDKFGSVVARRGKVRYDYRTSGSHRASDCAIFPVYNWVE
                     AGWSATAEVPGTGAEGRLNIGMMGIIIPHDSAVEDAAEPLLKALNRVGVTSTFYFDDD
                     DEPRKKELDLNGYGELFYHHLSDDTTQERVRDLAMAAQWMAVSVAGENAYVEQLLARL
                     PGEVSRSLSAQRDTGGWGVVLVEDDRVPEAWGTMPVIVISAVSREAAASAFRAISQSL
                     DTSSSIQLPAGTDFRTTKTPLDDYGFAVLTSGTGAATMEPDGTLTLFLGRTSDWAQKN
                     MIGREINPERRDMAFHYGFYGHAGSWRDGNVVRAGYEFNNPLTAVVPSRSTVARLPKT
                     QSFLSLTGTGAVVTAVKPAGNPLARFENTPSDPRNGIVVRAYEALGRSERIELRLFSG
                     IARASETDLVENDVTSVPVDGGAVARELAPFSINTFRVIPVEKDRSGGVQPSVRDWRP
                     DHVVWCRYYQHNVGAHPTGYLPVGIYIDGELPIENRGGQFPTVGRCRVWIVNNNTDRA
                     ISGTARLLAPDHWTLHPAEIPYELGPREHVTTEVVVAFDCRPRVGMIKARMEYDGVVY
                     QDVLEAGFKTVSRGVATERGAVRYNGWEVVKEREPEWTVLRNAEDIIVRIRNPWLQPL
                     EAEVAVISPMEMWGRDAGSYGLCALEPDTAAITLRPRETREIRFRVSVGEKPRPTFWA
                     WAKLMCNGKCAYLPVPGTTA"
     gene            18380..18452
                     /locus_tag="PLT86_04660"
     tRNA            18380..18452
                     /locus_tag="PLT86_04660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18413..18415,aa:Met,seq:cat)
     gene            18970..19173
                     /locus_tag="PLT86_04665"
     CDS             18970..19173
                     /locus_tag="PLT86_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT35936.1"
                     /translation="MATGTVKWFNHTKGYGFIRPESGEDLFVHVTNVDQADAKRISDG
                     DAVEFDVRPGRKGQEAYNVRLAH"
BASE COUNT         3873 a         5651 c         5685 g         4079 t
ORIGIN      
        1 ccgaaaaacc ggtggctacg gagaggaagc ggtagttcgt tttgctggcg gtgaacgcga
       61 tgatgctgcc gatgcctgtt gccatgccgg cgaacactgt gagcccgaac gcgagaagca
      121 cattgttcat acactgtttt ccctggtgat cttgcccgtc gccccgaacg ttcctcgaat
      181 cggctaccgt tcacgctctt tccagcgcgt ccaccggcaa tttcgtcaca ccgacctttt
      241 ccttcgtgat gctgtacacc acccagacgt tcttgcccac cgtcagggct ttgtagtact
      301 gcggaccgcc gaacttgtac atacccccgt ctggctcacg atccacgtga aggagttcgt
      361 acgtcctgtc gaaggtgatt ccgtcatccg agagcgtgat gaacatgacc ttccggctct
      421 tgtcagcggc gttgtagtag tcatcgaagc tgttgcagat gacccacacc cgcccgctcg
      481 gaatttctcc cgccaccggc atcgctccgc catacaggtt cgtctccact gccggtgggt
      541 aacagtcctc ttcccgcttt ttgagcgcag cccagtagtg gccccggcgc tcgaggttat
      601 cccgcagaac cacccacttc ccgtcgcggc ggcggaattc cgtctcgtga gcgagaccgt
      661 ttgtgccgtc ctccgtcagc ctgctcgtgc cgggtgcata tttcgcatgg cgcgcaaact
      721 ggactggcac cccgttcagg atgtcatctc gcatctcggt cggggcggca tcaaacggta
      781 acgcctccga atacggcggt aacaacggcg cgatatcctt ccaccttcca tcgcacacct
      841 cgaaccgcct cgtgaggggc gcctgggcgt acatcgggga atcggctgcg agaacgccgt
      901 cctgaattct ccaccgctgg acgaaacgca gtccgagatc cagccacagg tcgtagatga
      961 acccccgttc gcggtcgagg tcgtcgctcc atgccgaggc agggagcggt ttggtctgaa
     1021 acccgtggta ttgcacgtca ttggtgtagc catggcaggc aactatcaac ccgctggtgt
     1081 acagccttcc gttgataagc tgcaggccgc cgagcgcata agggtagatg atgtgcgggt
     1141 cgtggttcca ccatcgcctg cggaccggaa tcggcggcgg gatgatctcc accagcgcgc
     1201 catcccggct ccagtcgatc tcctcgaatg cttcgtcaaa ctgcccggcg tatgcgagca
     1261 tgcgttgacc tggcccgttc tcgtccttgc tgtgctgcgt ccagtacacg atgaaggtgc
     1321 ggtctttgag cacgattgcc ggatgatggc tgtaggttcc gtggcgcagg ctttcgtacc
     1381 gcccgtcgcc gccctcgtcc acgttcgcgg cgcaggggaa ggggtcgtac aggacggtgt
     1441 gtgctgctgc ggggaaaggt ggcagaccga ggtcaggcgc gtttgcgtcg aaggttgccg
     1501 gtggaaccca tgtgataggc gacggtgtca tgtgttggtt cctttgcggg aatcggattt
     1561 tcgggtacct gaacccatgt gcgcgggtcg cgttatctgc cgtgcggtat ccagcggaag
     1621 cttcgtttct cgtgcacggc cgcttccgcg gatctgatgg ccacccccca ctcgtcaaac
     1681 cttggcaaaa ccagcgaaaa actcataaac ttaaaacctt gtgtctgttc ttgatcgcgc
     1741 gcgcagcaga tggttcgcgc ggcttaggct gttgaaagga tacccaatgg cgtctcaggt
     1801 gaaaaacccc gcgctggccg agcagggcag gcggaacatt gaatgggcgg agcggcagat
     1861 gggcgcgctc ttgaaagtgc aggaacgttt tgcgaaagag aagccgctcg tcggcgtgcg
     1921 agtaggcatg gcgcttcacg taacgaagga aacggccgtg cttgtgaaaa cgcttgttgc
     1981 gggtggcgcc gaggtttcca tcaccggctg caacccgctt tcgactcagg acgatgtggc
     2041 cgccgcgctg gcgctggaac cgcacgtcga agtgttcgcc cacaaaggcg aaacgcggga
     2101 ggagtactac gatttcctca accgtgtcat cgccttcaag ccgcacgtga ccatagatga
     2161 cggctgcgac ctcgtctccg aaatccacac aaagcatcag gggctcatac cggacgtcat
     2221 ctgcgggtgc gaggaaacaa cgaccggcgt catacgcctc gccgcaatgg aaaaggattg
     2281 cgcgctcaaa taccccatga tcgcagtcaa cgacagcgac acgaagcatc tcatggataa
     2341 ttactacggc acgggccagt ccactctcga cggcatcctc cgcgcatcga acatccttat
     2401 ctccggcaag gtcattgttg tcgtagggta cgggaattgc ggcaagggtg ttgccagccg
     2461 cgcccgcggg cttggggcac gggttatcgt caccgaggta aaccccttcc gggcgctgca
     2521 ggctaccatg gacggatttg ccgtgatgcc gatggccgaa gcagtgcgcg agggcgaggt
     2581 atttgtcacc gtcacgggca acaagaacgt cgtgagtacc gagcacatca gagcgatgaa
     2641 agacggcgca atccttgcca attcgggcca tttcgacaac gaggtagacg tggcaggcct
     2701 taccgcgatg gcgaaagcga aacgcacggt ccgcccgatg ttcgacgaat acgacctcgg
     2761 cgggaaggtc gtgtttcttg cgggagaagg ccggctggtg aaccttgccg ccgccgaagg
     2821 gcacccctcc accgtgatgg cgatgtcatt ctgcaatcag gcgatggcag tggaatgggg
     2881 ggtaaagaac cggggcaagc tggaagcaaa agtctaccgt ctcccccccg aactggatct
     2941 ggaggtatcg cggctccaac tcgaagcaat gggcatccgc attgacaaac tgacgctgga
     3001 gcaggaggcc taccttaatt cgtggcagga aggcacctga cgaaccgttc ccggaaccgg
     3061 aacacggaaa aagcgcggcg acccgataag ggtgccgcgc ttctgttttt cgggaacgaa
     3121 gggaaacccg aagaagaagg caacgaaacg ctggcggaga gggagggatt cgaaccctcg
     3181 gaacggctca tcaccgctca cacgatttcc agtcgtgccg gttaaaccac tcccgcacct
     3241 ctccgcacga gatgcataca ctgcttcaga tcgggtaaga atggcggaga ggcagggatt
     3301 cgaaccctgg gagggcaaaa ccctcaacgg ttttcgagac cgcccctttc aaccgctcag
     3361 gcacctctcc gcgaaatcat cgccgtgacg aaaagaactc gcgcaggagc gcggcgcttt
     3421 cgtcggcaag cacccccgaa atgatgttga cgcggtggtt ccaccccata tccggagcca
     3481 acgcgtcgat ccgggaaccg catgcgccga atttcggatc cctcgcgccg aataccagcc
     3541 cgccaatccg cgagagcatc aacgcgcctg cgcacatcat gcatggttcc agggtgacgt
     3601 acagcacggc gtcattcaac cgccaggcac ccactgcctg cgttgcttcg cgcagcgcaa
     3661 gcatctccgc gtggttcaac gcgctttcgc ccgtttcaac ggtattgtga ccgcggccga
     3721 tcacgattcc gtcgcggaca accactgccc ctaccggcac ttcctgttca tgaagcgccg
     3781 cccttgcctc ggcaagcgcg aggcgcatcc aggtttcgtg atccggaatt ctatccagaa
     3841 gcaggcgtca tgtcctcggt ggaaatcgct ggacgggcag agaaaaaaaa gaagtgcgct
     3901 cagagggagt cgaaccccca accttctgat ccgtagtcag atgctctatc caattgagct
     3961 atgagcgcac gcgatcgttt ttgtgattat gaacttttta atatactgcc caaatcccgc
     4021 aggaacaagc ccgcgagggc cactccgtcc tatgtccggt catccatggg aacgtattcc
     4081 cgttcgtcct cgcccacata ttgctgccgc gggcggacga ttttccgttc ctcttcgttg
     4141 tccatcagct cgagccagtg ggaaagccat cctgcagttc gggcaagcgc gaacagcacg
     4201 gggaacatgt caaccgggaa cccgagtgcc ttgtaaatga tgccggagta gaaatctacg
     4261 ttcgggtaga gcttcctctt gatgaaatac tcgtcgctca gcgcaatgca ctccagttcc
     4321 ctcgcaacgt cgatgagcgg atctggaccc gtcacttcca gcacctggtc cgccattttc
     4381 ttgattatct tggctcgcgg atcgtagttc ttgtaaaccc ggtgcccgaa ccccatcaaa
     4441 cgcgtctcgt ggtttttgac gcgcttgatg aacgctggaa tgttttcagt cgatccgatc
     4501 tgcgcgagca tttccagcgc ttcctggttt gcgccgccgt gaagttgccc gtaaagcgcg
     4561 cccgccgccg ccgaaattgc ggaatagggg tctgaaagtg cgcttcctac cgttcgcatc
     4621 gtggtggtgc tacaattctg ttcatggtcc gcatgcagga tgaacagcac gtccagcgcc
     4681 cgttctatca ccgggttttg gtaaatttcc tccttacccg gctgcatgcg aaaaagcatg
     4741 ttcaagaagt tgtagatgta agagagcgac ggatcggggt acacgtaagg aagcccgcag
     4801 ctgtggcggt acgtataggc ggcgatcgtc ggaaccgatg cgatgatgtt gatcgtttcg
     4861 cgcattcgct gtttcgggtc cagaacatgt ttcgcggccg ggtagaacgt tgatttcgct
     4921 ccgagtgttc ccacaaaaac acccatcgcg tgggcattgc tgtggaaccc gtcgaggaac
     4981 ttcttgattg attcgtggat aagcatgttc ttgacgattt tgttcgtcca gtccgcaaac
     5041 tcggcgaacg tcggcagttt cccgtgtatc agcaggtacg ccacttccag gaacgtcgat
     5101 ttctcagcga gttgctcaat cggatagccg cggtatcgca ggatgccccg ctcgccatca
     5161 atgaacgtga tcgcactggc ggtcgtttcc gtgttggtga gcgccgggtc gaatgagagc
     5221 aatccgaaat catcctcact gaccttgatc tggcgcagat ccatggcgct gattgacgcg
     5281 ccatattcgg ggtacgtccc gtacctgatg gggatcacat aaaccttgcc ggtcctgttg
     5341 tcagttacgg tgagagtatc ctccgccatc ttttcgaacc tttcttcccc agttttcgtg
     5401 aacaaccacc attttcgcga cgcgtgcgat caaagcatcg gttccaggat cttgatcgca
     5461 cgcagtatgt caactcgttg ctgaggcccg tggatctccc gttcgatggt caagggccct
     5521 tcaaatcctt tggccttcag tcggcgtatc aacagatcaa accggacagc cccttcgccg
     5581 aggggcgtct cgtgaccaag cgcctcatcg cggttcggcc acacaccgtc cttggcatgc
     5641 agtcctcgca catactcgcc gaaaatctct tcggcgtcca gagggtttgc tttgccgtaa
     5701 agaatcaggt tcgccgggtc gagattcacg aaaagattcc ccgtaccgac gtcgcggatt
     5761 gttcgcttga gcgtcgacgg caactcctgt ccggtctcga agcagaactt ctgaccgttc
     5821 cgtttgcagt gaagcgccag atcgcgcatc gcgtcgacga atgtctcgta taccgggtca
     5881 cgctcgtcgt ccgggatgaa cccgatgtgg caggtgatgc tgtcgatccc catgcgcgct
     5941 acccagtcgc tgaaaagttt tcccgatctg aggcgctcat ctcgaaagcg aggcgcaacc
     6001 agccccattg tggacggacc gttgacgttg tcaaattgct ggccttccca gagcagaaac
     6061 acagaactga tccgtacacc cgccttgtcc gcagcgttgc gaaccttctc cgggtcggaa
     6121 tacttcccgg cgagcgctgc ctccgcggtt cctgtaacct ggcatgtcgg aatacccagc
     6181 tcagcgacct gtctgaacga ctcgttcgga tcgctcgcga agcccaccat gagccccagt
     6241 tccagctttt tcacagcgcc atccttgtga aaaggtccac ggaagagaag agacgtcagt
     6301 tcgatttgtt cgagtaggaa acgcgtaccg aagtgcgaat gccgccccgc gggttgaaat
     6361 cagccacaac aaccgcgcgc cgcggcttgc aggcggctac aaactcgttc agtatcttgt
     6421 tcgcgacgtt ctcaaaaaag atacccatat tccgatacgc ctgcatatac agcttcaggg
     6481 acttcagctc aacgcaaaga gcatcgggtt catacacgat ggtgatcgtg gcgaaatccg
     6541 gttgcccggt tttcggacac atcgaagtga actcgggaat cgtaatcctg attgtgtaac
     6601 tgcggtcgcg atattgattt ggccacacat caatagcagg tgtctcgccc tgtgatttcg
     6661 catggttttc gtcgtacccc gtagttgtcg tcactgtccg cttccctcct cacaggccgg
     6721 cgaatgcgca caacgcatcg ctcaaaatgg cgtccgtttc caggtcaaca tcgataccga
     6781 tcacatgcgt tccactggca ggaaaaaccc attctgcgtt ctcgccaagc ccgtgcgcgg
     6841 gcatgaaacg cagcgaatga cgctccgggt gtatggctac gtccaccgca ccgatgtccc
     6901 agaattcctt tggcgggtta tccgagaggg acctgcccga tctggtggtc cattccacgg
     6961 ttcgatccgc cagcaacgta tgtatcggtt ttgccatttc ccggagctgc cccaccagcc
     7021 actcagttcg ccacacgagc ttctcagtta cgccccaggc gggaacatgg aatagcgtgg
     7081 ataacatcgc ggcacggctt tcgaaaagcg ccttcacggc ccaggggtcc cccagtgcat
     7141 tccattctga tccgcgtcgg cgaaattcct cctcgtcccg gaatccaccg agccatatga
     7201 aacgcgcctt cgacgcgatc tcaggggcgc gaagaagcgc ggaggcaaga tcagttgccg
     7261 gccccgttcc gatcacgaac aacggctccg gatcttcggt cagagcacgg tcaatgatga
     7321 aatccacccc ttcagcggag cccgggctca cactgttgtc gaggggacgc tccacacccc
     7381 gcagattcgg gacctcaggc cgcccgagag ccgccagaat ggccgccgct tcttcctggt
     7441 agatcgttac cgattcgggt ccgtttgaat gcgtattctg aacattgacg atcgcctcaa
     7501 tccgcacacc tttcgcgaac gcatacgtga tcgcgaactg atcgtcaatt tcgtttttcg
     7561 tgtcgcaatc aaggatcaca cgcattgtca cgctgccttt tacagaagca cgtcagcatc
     7621 gccgcgtcta acgtgttgtg ccgcaaggca cacccgcgcg aaatagccgt caacagacgg
     7681 caggaaatct gcatttgtca gaacccgagt accggttgct ggcgcattgc ccggattcgt
     7741 tgagtagaaa actggagcaa ccaaccggtt cacgcggccc agaaaaagct ccaacccgtc
     7801 acccgccggg acgtggacga tcgctgcaat ttcctccgcc gcaaccgagt agtcttcgag
     7861 accgcggttg tcgacaatgc cgtacaccgt ttgaatctcc cggtccacga actgcccgaa
     7921 gcggtgcgca ctctggcgca cgccgaggaa acgtaaccgg gaagccggaa cgtggaggcc
     7981 aagttcttcc cccagttctc ggaccgctcc cgccagagga ttctcccctt tgttgagatg
     8041 gcctccgacg gcaacgtcta tcatgccggg agcgatatcc ttccccgcgg agcgctgctg
     8101 aaacagaacg tggccatctc ccagaccggg cgcaaatacc cacacatgga cagttttgtg
     8161 ccaaaggccc tgttcgtgga cttccgaacg tgttgcctcg gccacgtgac aaccgttgtc
     8221 gtcacatacg tccagcaact catccgacat ggggttcttc ttcgcggttg acggtgacgg
     8281 cccatgcggc ggcaaattca tcctcagtta cgtcggagac gactgtagcg tcgccaatgc
     8341 cccggggcat gacgaaacgg agcaccccac caagggattt cttgtcgcct ttcatggcat
     8401 tccagatgtc acggggtgcc atgtcggttg gcgggggaat gcggagcagg cgccggagaa
     8461 tccttcgctg ccgaaagagt tcgtccgagc gccacaggcc ccgttcgcac gccatccgcc
     8521 cggctaccat cattccaaac ccgactgctt ctccgtggcg atagcgctgg taagctccca
     8581 gcttttcaat cgcgtgtcct gcggtatgtc catagttgag gatggcacgg aggccggatt
     8641 ccttttcgtc ctgaccgaca acggacgcct tgatccggca atttgcggcg acgaggtcta
     8701 tccacgaatc ggcagaagcg gttcctgcca caaaagcatc cagcgaggcc tcaagcagat
     8761 cgaacaaacc cggatcgcga attactgcgt gtttgaggac ttcaacgagc ccggcccgca
     8821 ggtcgcgttc cgaaagggag gccagcaggg cggtgtcgac aaacaccaag gacggttgat
     8881 gaaacgcacc aatcaggttt ttgccgagtg gatgatcgac acccgttttg ccgccgacgc
     8941 ttgcgtcaac ctgagccacg accgtggtgg gaacttgaac gaagggcagt cccctgaggc
     9001 agatggcggc gacaaacccg gcaacatcgc ccgcaacgcc gccaccaaat ccgatcacca
     9061 gactttcgcg gtcgaagcct gcggccagga agtgatccag tattccggcg accgtgtgaa
     9121 gcgttttgtg ttcctcgccg ggctcgatga tcttcgggac aacgcggaaa cccgccgcct
     9181 caagactcgc tgagcactgg tctccgtaaa ggctccatac tgttttgtca gaaacaacgc
     9241 cgacaaggcg ccccgccgtc accctcctta ctgacttccc caacgtgcac agcatggaac
     9301 tcccgatgac tacgggatag gttctgctgc ccagaggcac ttccacggtt tcggttcttg
     9361 ccacggaaag gcacctcgta cctgtaattc gcggaaggcg ggtggaacgc aaagcgcccc
     9421 gatttcccgt cggaatcatc ggggcgcgcg gcaaaaggtg gcgcccccca gaatcgaact
     9481 ggggacacag ggattttcag tcccttgctc taccaactga gctagagcgc catgaaagac
     9541 aattaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctca ttcgctcgtg
     9661 cgagcgctct gtcgaagggc ctgcaaccgg tcgcgcaata cggccgcccg ttcgaaatgc
     9721 tccgaagcga ttgcctcgcg cagttcgcgc tccacccgtt gctccagcgt caatggccgc
     9781 gtctgagaga tgtcttccag ccatttcttg aggtaactga tctcgccgga gttctcgatg
     9841 gcatcctctc ggccctgttc ccggtagatt tcagcgatgt cttcaattcc gcgattgatc
     9901 cgctctacgg ccccgtcaaa atccttggct tcccacgcct ggcgcgcgcc cgcgagaaac
     9961 ttgtggacga gaatgaatgg tttccattgc gtgaacattt cgcgcagctg gtcgttttcg
    10021 gcgtacttca cgacgaaatc gatgattttc agattgtggt ccgcatcctc ctctgcggcg
    10081 cgatactccc ccagctcaag ccaactgatg cggcgatggt agaactgcat gctttcctgc
    10141 tgcaggcgca cacaatcttc ctcatccaat ctgaactcgc caccttcgtg ttccccgcca
    10201 ggtgcttcct ccgcaaggtg catgtagtac tcgagtgcag tggtgaaacc gtgaggtctt
    10261 ttgccgtcag gccttccgga aatctccatc tggaggaggc caagatccag ccgaatctgg
    10321 aggcgaggcc gcccgtcgtc gccgacaatg cgtctcaccg ccggccgatc cggtcggaag
    10381 tcccaccctt cgaggattga ggaaatgtct ttgctcacag aaccctcgtc agcctgaata
    10441 ataccgtcct ccagaaaccc cctccctgtc aggcgttcga aattaccgtg gagtgtgccc
    10501 gccacaaggg gttccagcat ctggagtgca cgtacaaaat atacgaagcg agagttccta
    10561 ttggggtttc cgcgtgcggt tgcgcacctc gccggtcgcg aagcgagcgg ggaagctcat
    10621 gtccgcgacc ggagttcttc ttactccgta agaaactgcg tgccgtcgta cgccagtttc
    10681 ccgttctccc atatctggcc gccgcgcagc tcgtaccgca tgtactgccc caccgtcatg
    10741 tggcgcatca cttcgcgggg gtccttgtac ttcgccgcga ggacttccag gggcccctcg
    10801 aagcgcgttc ccggctgcgg tttcgtcgcc tgtttttcga caaccttgat ggggaggctg
    10861 gaagaggtat cctgggcggc aaggcgctgc tgtttccggc gaaagcggtc gtgatcggtt
    10921 tcgtcctcgg tgcgttcagt ttcggggcgg tgggccggct ggttgtaaga tgtttcctga
    10981 acaggcgggg cctggttcag tctcctgaga atctgcgtgc tgcgtgccat cggcaatctc
    11041 ccgttcaccc ggctggcgcg cgccagtctc ggggacgcga aaaaaaggat cggcgcgttt
    11101 ccgtgccgat cctcgtttcg tacgttgaag gagccccgga cgcggaagca tccgaggccc
    11161 ttactatcgt atggggtgag tgaggggatt cgaacccccg accctcagat ccacagtctg
    11221 atgctctaac caactgagct acactcacca tggttttccg gttgtgcaca tggacggttt
    11281 cctgaactgc cgtcgcccgt ggcagcagga agacagctag ttaataatag cgaacgaaac
    11341 tcaccgagtc aacgaaaccg gcgcgtcaga ctgtgcccgc tcgcgcggtg caggcccttg
    11401 gggcgtaatt gctgcagata tctgtttcca acactcccag gttcctcagt tgtgactcga
    11461 tttccgcgat tttctcaggc ccgccatagt gcatctggcc ggggtggtgg ccgtcggaac
    11521 ccaacgaaaa tttcattccc tcgtctcgtc ccatcctgat gatcttccgg aagaattccg
    11581 gcatgatccc gagacggccc ggattgatct cgaatgcggt gcccttggcg gcggcctccg
    11641 cgaacagcct gcgaacacgt ttcatgtcgt atgcggccat gagccggggc cagtctatct
    11701 cccgcccgtc ttccattgtt cggaccccga cgtacgaaaa ggggtgcggg atgatcgaag
    11761 caccgtaccg aactgcaccc tctgccattg taaggaaatg gtccgcatat ccctcttcag
    11821 tccgttcgtc cggttggctt acgcacggga ggtggaaatg attcgcagag atgattacat
    11881 aatcccatgt cgccatgatt tccggttcca ccgcaggcag gtccggtgcg ttcatggaga
    11941 cttcagagcc tatgagcacg cgaacggaag tgtcgagttc gtcgcgctct ttcatgttct
    12001 cgacgatcag aggttcccga ccgctccccg gttcgtctat gtgatcgcag acgccaatga
    12061 gcgtcaagcc gaccgattcg gcctccgcga cggtattcgc gatggtgctg tctcgccctc
    12121 cgcagttgga acggtgtgtg tgcgtatgca tattttgcca tacgagcatt cgatgccctt
    12181 tcggttgcag gcggatcgac acgtggtact ctccaaatgg taaacctgga cagttccctc
    12241 gcacaacgac tcccgctgaa tggcgtggcc tcgtgacggt ctccggggcg ctcctgatcg
    12301 atcattttgt ccggcagtgg gcggaacttc acgaacgcga gtgggaactc acacctctcc
    12361 atactcttac ggagaaagca cgccacgccc ttgggacgga tggttgccgg gccgcgcttg
    12421 ccttgctttc cgtatggaac cagcatcagc cgaagtttcc gacgtggagg catctggcgg
    12481 ccgaccggca gagcgctgaa caggcgagcg gcgagcgggc tgccaggtgg cgcgcggggc
    12541 gactatccgg gtgtacgcgc attctcgacc tgtgctgcgg aatcgggggc gatgccatcg
    12601 ctctttcgtc gcattgcgag catctcgtgg cgatcgacag ggattttcag cgggcgctct
    12661 gtgcccgttt caacgtgagt tcattcggcc acggcgaccg aaccgatgtc gtttgctcag
    12721 atgtaggagc ggttctaccg gcagcagaag ccgccgtcct tgatcctgac cggaggcccg
    12781 gcggcaggcg ggtgattcga ccggaactct acgaaccgcc agcgtgccta tgggcgcaga
    12841 tccgtaaatc tgtccggacc cttgccgtga aggtggcgcc cggaatccct tttgatgcta
    12901 ttccggcgga tgcggtcccc gagttcgtgg aggaccgcgg cgagtgccga gaggcggttc
    12961 tctggtttgg tgagcgaaaa ccgcatgcaa ggaccgccgt cattctcggg gcgggctcca
    13021 cggtgacggc ctcctgcatc gacgcttcgg cccctgagtc cttccaaaaa ggtgaggtcg
    13081 gaaggttcgt gtacgacccg tcgccggcgg ttgttcgcgc gcaactcgtc actcacctcg
    13141 cggcgaagct ttccgcgtgg cggctggacg atcggattgc ctacctgtct tcagattcgt
    13201 tggtggagac gccatttgcg aggggttttg aggttctttc cgtcataccc ttcagtctca
    13261 aaagattgag agcacatctg cgcgataaga gagtgggcgc cgtggaaata aggacgcgga
    13321 ggttccctgt gaatcccgat gaactgaggc cgatgttgga cctccgtgga ccggaatctg
    13381 caacggtgat tcttacccgt gtgcgaggaa atgcactcgc gattgtctgc aggcaaggga
    13441 attccggcga tctcatttga gcagacccct gcggtcagat aattgcgatg ggaacgcctt
    13501 tatttgtgtt cgatgcaaag agtcatacca acaggagtcg cgcatgaatg ccgaatacgc
    13561 gtgggtagaa ggtcgccctg ttgtcagggt tgattcgctg ggcatcaacg cgtttctcac
    13621 cgactggctt gttctcgacc cgtttccgtt cgaattgccc ggcgggctga accgggaact
    13681 ctggatcggc agggcagacg cagttgacgc tgatccgacc ggcgtggaga ggacggttcg
    13741 accgcgtgaa ggggacaagc aacgcaacga tgccgtcgcc ggcggcttct cggtctggca
    13801 gcgacttatc ggttccacag tccatgattt gaaaagccgt tatccgggct tttccagtgc
    13861 gctggtgtac gcggcgacgt acctccacgc agacaccgac acgattctgg ccttctgtat
    13921 cgacgactgg gagatatatc gctcgtgtac ggcacagttg ctcctcgatg gcgtggaggt
    13981 gtgctttggt ccttcgcccg ccgtggtaag gattccggcg ggcacgcact gccttattgc
    14041 gaagatcggc ggcggcagcg ggagcgggtt tgcctggaag ctttcggtga gggcgggacg
    14101 tgtggagacc acacctgccg gctggggtgt ttcactggcc cgattgccgg ggttctggcg
    14161 tggcgaggaa tgcgctcctg aggtagaggt tgacgtggtg cttgtcaacc cgaccggttc
    14221 gcctctcgaa gcaggcaaaa ttgccgcgaa cctttgtgga caaactcagg gagcgccgag
    14281 cgagaaccat ttcctcgacc catggcaggc aaaagcaatc agaatcagcg ccccgctggg
    14341 cgcgtgctca cccgagtcca cgacgaccgt tgaactcgcc ttttacggca gaaccgttgc
    14401 attcccggta acggtacccc ggctgcttcc tccctgcacg atccacgtgc tggagggatt
    14461 tcattgcgac cccgtctggg tgagcgatca gagacactac aacctgattt ctctggaaaa
    14521 cgtgaaaatg caggtggatg cgtgtctcgt ggacccggga tatcgcgcgt tcgtgcacga
    14581 actcgattac ttgaaatcgt ttgtggacga gtaccccgat tatcgcgcca aagtgttcga
    14641 actcgttgcg tcgggccagc tgattgtggg cagttcgtac agcgaaccga atgaaaacaa
    14701 tatctccggc gaagcgatca tccggaacat catctacggg caaggttacc accgccattt
    14761 tctgggcggt gacccgaggg tgtatcacgc atgggacgtg ttcggccaca ttcctcaact
    14821 cagccagata cttgcgcgtt ccgggcaccg cgccgcgcta tggtcgaaaa gcatccttgg
    14881 cttcccgccg ctgttccgtc atatgtcgct tgacggaacg gttctgccgc acatcaggac
    14941 gcactacacg tggtacagca acagcctcga caacctgcgg accatctcgg aaccggttgt
    15001 tcgggagaaa caatcgtttg gctggcaccg gcatctgatg gtagaggctt ccgatttcac
    15061 gccaccgagt gcgtggatga tcggcagaac cgccgagatg gccgcgtcat accccagaat
    15121 tctcatgacg gggccggaag agtttctcga cgggctgaca gcagacgggg cgcgccttcc
    15181 gctgacgagc cgcaatccct cgcagcatca cgtgggaaca ttccacacgc gagtggaact
    15241 caaactggca aaccggcttg cagagaacat gctcttcgcg gcggaaatct ggtctacgtt
    15301 ttccggtata ctcggggcgg agtatccgga ccacctgatt gacaaagcgt ggcgtcagct
    15361 cttgttcggt caacaccacg atgccatcac gggaaccccc tgcgatgttt cctacctcga
    15421 tttgatggcc ggataccgcg aagccctcag gctcgcacgg ggagtgctcg aagacgccac
    15481 aacgtacatc gccgacgcag ttacggtttc gcgggacggc acttcagtcg tcgcattcaa
    15541 cccgctcgcg tgggagcgcg acggcgtgat tacgatccag aaaccggagg ggtttgaagg
    15601 aatagaggtc aggaatcagg atgggacgtc tctgccttgc ttcgccaacg agacaacggt
    15661 cgagattctg gccaagaaga ttccgagtgt tggctattcg actctggaac tcctgcccgc
    15721 gcctgcgcga tctgaaccca cggttacccg gtgctatgtg cttgaaaacg aatactggcg
    15781 tatggaactc gatcccgcgc gtggtggggg aatcgtgagc ctgttcgaca aggaacaggg
    15841 caaagagatc gtcgatagtg ctctcggagt cggaaatgat cttcttgcac tcggcaatgg
    15901 acccaatccg tgggagtttc acacgaccgg tgagcgggtt gcggcatccg attctcccgc
    15961 cgttgtagaa gtacatacca cgccgacttc ccagaccgca gtggtttccg gcagactggg
    16021 agagatttgt tcgtaccgcc gcacgctgac actgcgcccc gggagcagaa cgatagaagc
    16081 gtccgtagtt ctttctggtt accgggccaa cgggcatcag ttcgtagcga cttttccgac
    16141 cgcgttgcag ggcgcgcttc cgattttcga agacaaattc ggttccgttg ttgcgcgcag
    16201 gggcaaggtg cgttatgact accgaacttc cgggtctcat cgtgcctctg attgtgcgat
    16261 tttccccgtg tacaactggg tcgaggccgg ttggtcggca actgcggagg taccgggcac
    16321 aggtgccgag gggcgtctga acatcggcat gatggggata attatccctc atgattccgc
    16381 ggtcgaggat gccgctgaac ctttgctcaa ggcgctgaac agggtcggtg tcacctcgac
    16441 gttctatttc gacgacgatg acgaaccgag gaagaaggag ctcgacctca acgggtacgg
    16501 tgagctgttc taccatcatc tttccgacga cactacccaa gaacgagtgc gtgatctggc
    16561 aatggcagcg caatggatgg ctgtttcggt tgccggtgag aacgcgtatg tcgaacagct
    16621 tctcgcccgt ctccccggcg aggtgagtcg gtcgctctcc gcacagcgcg acacgggtgg
    16681 ctggggagtt gtgctggtgg aggatgatcg ggttcccgag gcatggggga cgatgccggt
    16741 gattgtgata tcggcagtat cgcgagaagc cgcggcgtca gcgttccgtg ccatcagcca
    16801 gagtctcgac accagttcga gcatccagct tcccgcagga acggatttcc gcaccacgaa
    16861 aacgccactt gacgactacg gttttgccgt tctgacctcc ggcacgggcg ccgcgaccat
    16921 ggaaccagac ggaacgctca ctctgttcct cggtagaaca tcggattggg ctcagaagaa
    16981 catgattggg agggaaatca acccggagcg gcgggacatg gcattccatt acggattcta
    17041 cggtcacgcg ggatcgtggc gcgacgggaa cgtggtacgg gcagggtacg agttcaacaa
    17101 ccccctaacg gcagtggtcc cgtccaggag cactgtagcg cgtcttccca agacccagag
    17161 ttttctgtcg cttaccggta cgggagcagt ggtgaccgcc gtgaaaccgg caggcaatcc
    17221 tctggcgcgg tttgagaaca caccgagtga tccccggaac gggatcgttg tccgggcgta
    17281 tgaggccctt ggaaggtccg aaaggatcga actgagattg ttttcgggca tagcgcgcgc
    17341 gtcggagacg gatcttgtgg agaacgatgt cacctccgtg ccagtggatg ggggtgcggt
    17401 tgcgcgcgag ctcgcgccat tttccatcaa caccttcagg gtaattccgg ttgagaaaga
    17461 ccgaagtggg ggtgttcagc cttccgtccg tgactggcgg ccggaccacg tggtctggtg
    17521 ccgttactac cagcacaacg tgggagcgca cccgacaggg tatctcccgg tcggcatcta
    17581 catcgacggc gagcttccga ttgagaaccg gggcgggcag ttcccgaccg tcggacggtg
    17641 cagggtgtgg atagtgaaca acaacacgga tcgcgcgatt tccggtacgg cccgcctgct
    17701 ggcacccgac cattggacgc ttcatccggc ggaaatcccg tacgaactgg gtcctcgaga
    17761 gcacgtgaca acggaagtgg tggttgcctt cgactgcagg ccgcgcgtcg gcatgataaa
    17821 ggcccgaatg gaatacgatg gagtggtata ccaggacgtt ctggaagccg ggttcaaaac
    17881 cgtgagtcgc ggagttgcta ccgaacgggg agcagttcgg tacaacgggt gggaagtggt
    17941 caaagagcgg gaaccggaat ggacggttct gcggaacgcg gaggatatca tcgttcgaat
    18001 tcggaatcca tggctccagc cgctggaagc ggaggttgcg gtcatcagcc cgatggaaat
    18061 gtggggtcgg gacgcggggt cgtacggttt gtgcgccctg gaaccggata cggcggcgat
    18121 aacattgcgt ccgagggaaa cgcgcgagat tcgtttccgt gtcagcgtcg gggaaaagcc
    18181 gaggccgacg ttctgggcgt gggcgaaact catgtgcaac gggaaatgtg cgtatttgcc
    18241 ggttcccggc acgaccgcgt gatggccgga atcatggcat ttgaggaacc gcagaacagg
    18301 gctgcgtaat ggacgcacga ttttcttcca ttcgggtgtt gacatccgat gccagggtgc
    18361 gtatacttaa ctgtcccaac gcggggtgga gcagttggta gctcgccagg ctcataacct
    18421 ggaggtcgga ggttcaaatc cttcccccgc taccttgatc acaacacctg tcgcattgtt
    18481 ttgtcgcgcg acggagagta tagggaacgc gtaaggttga tcacgaccgc tgtttgcagt
    18541 ccttgggttg cttcatccct cgggttcctg acgatctatg atcatgctcc tgcgtacgcc
    18601 tgtgccgtga ggtgcgggcg ttcttttttg cagcctgccg cgtccaccaa gccgtggttt
    18661 ccctcgagcg ggttgcatgt tcgcgaagaa ggaggtgggg gtagagcata gtcgtctgtg
    18721 gttcgtgtag agcacgcgtt ttggtaagga agactacctc tttggacgtg tatcagcgtg
    18781 gaatttgtcc aaccgtcgtc ggtatccggc agaacggcgc aggttcacgt tgacagttcg
    18841 atcacaaggg ggaaggctgc caaaagtccc gcgtgagcgg ggtagtaagt ctgtggtcga
    18901 gaacacgcag ggctggagcc ctgtgcactc gtcacgcaac ctgggcaatg cccgaggaaa
    18961 ggtcagcaaa tggcaactgg aacagtgaag tggttcaacc acaccaaggg ttatgggttc
    19021 atccgtccgg aaagcggtga agacttgttt gtgcacgtta cgaacgtgga ccaggccgac
    19081 gccaagcgga tttccgatgg cgacgcggtg gaatttgacg ttcgtccggg ccgcaagggt
    19141 caggaagcat acaacgtccg tctggcgcat taagccctga cgaatctgcg caatgcgccc
    19201 gtccttgact tttcaggggc gggcgtgttt tttgtacgca gttcgtctca acaatagtcc
    19261 atttgtctct ctattgtatt tgagagcgat gccgacgacg gttctggcga agctccaaac
    19321 tcgaacgcac gatgtgccgg agggatgtca catgattcgt cgtacacgaa caggagtgtt
    19381 gtatgggg
//
LOCUS       DAOSOO010000040        19159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2876_length_19159_cov_5.601340, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000040 DAOSOO010000000
VERSION     DAOSOO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_19159_cov_5.601340"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..862)
                     /locus_tag="PLT86_04670"
     CDS             complement(35..862)
                     /locus_tag="PLT86_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT35937.1"
                     /translation="MHNQFADIRFMSFNLRFPCESDTGNLWDERRARVFEMIQLYDPD
                     VIGMQEVYRRQLNDLLSALPGYAAVGKERYGGTDEEHNPVFFRRERFLAEGSTTKWFS
                     DTPDVPGTRTFCPSDHPRIVTSATLMDIADGFRFSVLNTHFPLTRDEDIQLACARVVE
                     SQLALVPKSAPVVLMGDFNAPPDGGLHGFFTRSGGGIGEGFRDTRVLAQEVLGPAGTV
                     HGFRGGMLPESRRIDWVLLAGEGQVTRYETVAFERDGKYPSDHFPVCVDMRFAQPEN"
     gene            complement(873..1685)
                     /locus_tag="PLT86_04675"
     CDS             complement(873..1685)
                     /locus_tag="PLT86_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT35938.1"
                     /translation="MSVSEAAIVGVIQGMTEFLPVSSSAHIVYAEHFLGIKEGNVDLA
                     IVVHLGTLVAVFGAMWYRIAPIIRGTIAGVADLAVRRSPWAREEFRRATYIIAGTVPA
                     ALLGVLFEDSLKETFGNPFQTSVLLIGTGIILFATRFVRRNDRDWGLSPALAVGFAQA
                     LAILPGISRSGSTISMGLFLRIEREKAAEFSFLLSIPVILGGAIIHVAGMVGQPGGLA
                     LLSGPLIAGFCTSAISGYAAVRVLVSFVKKGALHWFAVYCWIVGAAGMWYFA"
     gene            complement(1720..2508)
                     /gene="pgeF"
                     /locus_tag="PLT86_04680"
     CDS             complement(1720..2508)
                     /gene="pgeF"
                     /locus_tag="PLT86_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOT35939.1"
                     /translation="MFRLTSETGLPMLRSSLLDELPGVWHGFTTRIGGVSAGSFAFLN
                     LSLNVGDSPDAVLQNRSLLARSAGISPEMVTLQAQQHGSDVVLCDSVSPEAARRADAF
                     IATREAIPVLVGTADCLPVLLASEDGRIVASIHAGWKGVLAEVIPRTVTAMQTVSGAR
                     PESMRAAIGPHIRACCFEVGDDVAHQFRKEHVVAPHARGGKSYVDLSAVAVCQLADSG
                     IAAKNIDISSTCTVCRHDLFFSHRANGGTTGRTVAMIMRRGDAH"
     gene            complement(2510..4789)
                     /locus_tag="PLT86_04685"
     CDS             complement(2510..4789)
                     /locus_tag="PLT86_04685"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT35940.1"
                     /translation="MNVPEETEKARAETAQCDRLFAEIMQLASRFDKNIDLPTLRRAY
                     RFASERHKGHFRSSGEPYLVHPVHVARILVDLHLDTQTLAAGLLHDVVEDDLKTPIEE
                     IEVNFGNDVATIVDGVTKISRMQLVSDKENQIDNFRKMLLAVAKDVRVILVKFADRLH
                     NLRTLDYLPPEKRLRIARETLDVYAPLAHRFGMARVRRELEELAFRYADPEACARLEA
                     QVAQRKDEYWNAIEQFCEEIREGLSKRGMPAEVSGRVKHLYSIHNKMVSEGKDLSEIY
                     DLLGVRIIVETADQCYDALAYLHQHYRPLEGRFKDYIGNPKSNGYQSLHTTVTNNQRT
                     VVEVQIRTREMHEVAEEGIARHWLYKEGRATGEIDAQMRWVRQFLEWSKDIRDPNEFY
                     EQLKIDLFPDEIFALTPKGDVIPLPKGATALDFAFAVHTEVGLHASGAIVGGRIVPLG
                     YELQTSDSVRIITSANQKPSRDWLNLVKTAKARTRVKQWLRQAEFALNVEIGRDLVEK
                     RLKKAGLVLAEEEWGAVASAFNTKDREHLFAAVGAGDLAPERVFDHIRSMIAPAPTKE
                     EAPPKELPATDSEAGGIVLSGMDNPLVRIARCCNPVPGDPVLGFITRGRGLTVHHREC
                     PNIRQLKEDAGRLIPVRWEAKDVIGGAGPFKTAIIVKGSDRVGLLSNVTSTIASTGAN
                     IRHAEIRTLPDGAHSAFLLEVKNLKQLKRVISKLRKVPNVVEVHRLATEGDDEDELRA
                     MIAGSPLPFVEESPDDEDE"
     gene            5099..5452
                     /gene="erpA"
                     /locus_tag="PLT86_04690"
     CDS             5099..5452
                     /gene="erpA"
                     /locus_tag="PLT86_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOT35941.1"
                     /translation="MADAPVAVDSLVTISESAAKKIRELLQEEGNPNYALRLQVVGGG
                     CAGFQYKLAFDDEFAEDDELLDYEGFRVVVDPMSARYVEGATIDYVESEMASGFKIEN
                     PNVVTTCGCGHSHSF"
     gene            5703..8267
                     /locus_tag="PLT86_04695"
     CDS             5703..8267
                     /locus_tag="PLT86_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT35942.1"
                     /translation="MNSIAHGLFRRVAEKWVAFSLRHLWLTAAVSLAFTGLALWAASF
                     LSLKTDLATLLPRDYPSVKELDRVQQKVGGLEKVIFVIESPDSAANHRFALALGQKLL
                     ADSAYSYVEWSRDLSFFDDHKLLYMDLEDLGEVYRRVSSKVQMDEFGEPLDFSDIEEK
                     YKGTNGSGNNSNGSDGWATADGTTRILIAYPAGQSGDVGFSRKVIASARKQVAALNPA
                     SFHPQLEVYYGGEFKNRVDEYESLVSDIKSTAIISILGIIITISLYFRQYFAWLYIGY
                     PLIAGLGWTFGITALTVGHLNLITAFLIAALTGLGIDFGIHVFQRYTEERGRGQDLET
                     ATTRAIVNTGSALLTSATTTVVAFYALTITDFKGFSEYGFIAGTGVLMTLTAVLFSFP
                     AFIAIGERLNLVRHRHAAKKWAESTATFPFAKAVVYGTVFLLAIAVFAAAFYPRVRFD
                     YDFSNLRSNIPASNLVKSKISKIQPMESAPAAIVVEDSAARMEVADALRAIQRADTLS
                     PTIKEVRTLEGFVPADQDQKMALIRRLRRVVEGHEEAILEGQSNINVEGLKRWLNVNE
                     ITTSDLPDFIVRKFRGPDGSLGEFVVIGTSVSLRDGKQTIAFAEDIREIDTPHFGKFY
                     ASGSAVIFADMLLVMQKDSVIATTVTLLVVFAIVLLQFGTKRAALLAILPVLVDGVLV
                     AFSKITGAGFHAKFEELAWVFGAEAVLGALLVFLFERKAFKTTILVMAPLLGGVILMI
                     GIMALTGVRFNFYNIVALPSILGMGVDNGVHFYARYREEGRGSVLYVLRTTGAAALMA
                     SLTTMEGFGGMLTADHRGLYSLGLISIIGMGTCLYMSVVFLPAWFAYREERHRKTD"
     gene            8298..9737
                     /gene="mpl"
                     /locus_tag="PLT86_04700"
     CDS             8298..9737
                     /gene="mpl"
                     /locus_tag="PLT86_04700"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOT35943.1"
                     /translation="MLVPSADIRHLHLIGICGTAMGALAVLLKERGFRVTGSDNAVYP
                     PMSEVLRDHDIPVMSAYSRDNITPPPDIVVIGNALSRGNEEVEAVLERKIPFVSLPEA
                     LREFFIRGSTSIVVTGTHGKTTTSSLLAWILDFAGLQPGFMIGGVPANFGVGARAGSG
                     RFFVSEGDEYDTAFFDKRSKFLHYLPDIVIVNNIEFDHADIFADIGEIELSFRRLLNI
                     IPRNGLVVANRDDSRVVRLTEHTWPPKVTFSLSGDANWHARNVCDTACGTTFDLVGPC
                     GQITCELPLFGRHNVRNALAVVAVCAHVGVSLEQIRDGMRSFKGVRRRLEKLFDAHQI
                     VLYDDFAHHPTAITETLRGVRAAHSGSRVWAVFEPRSNTTVRRFFQTELADALACADV
                     VRLGAVHRNDRIPASERLDVARLVSDLRERGADAESVPDVSRLSEEIASHARAGDVIV
                     VMSNGGFGGLTGLLRDALEKRFGASESAT"
     gene            9747..10250
                     /locus_tag="PLT86_04705"
     CDS             9747..10250
                     /locus_tag="PLT86_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35944.1"
                     /translation="MLSSPNWTIAERHMVRYHMSHLMWWALIAISVPVIALSLGAALV
                     KIAVLPPRGAGFLIWGATAAIAVLSPVVAFAIERGRIRPVPADRPLRAVVMWYKHAKL
                     WAIAACGIGALSPVSALYISGSFSPALGAVVFPLAFLMIRFPHAERFTAFVDSHFDAV
                     EPSQGPE"
     gene            10450..11283
                     /locus_tag="PLT86_04710"
     CDS             10450..11283
                     /locus_tag="PLT86_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35945.1"
                     /translation="MRTILLAAIAAACVVCAWSQASAAVIVTTSGEEIVCEIVRETDT
                     YFVVDHRGYRRYVLKTQVRSLDRRAGEGFNLGPEPLRNIEFSGGYRLWGRSQSHGIGA
                     SAISASYPLNENLLVQVGVEAGRGEASSSFEGGDLLPGPISWVGGMCGAQWQLQPWRV
                     RPFLDAGIGYFAMRHSVADSGVLWMKQAIAAIDGDTSFVYSEPMDGAFAVRLGVGALV
                     PLSGRFALTLRTSILVLHTGFTRQWYYPTSAEQEEMTLRSSHGITLEMIHTTAGVQWA
                     F"
     gene            11331..12209
                     /locus_tag="PLT86_04715"
     CDS             11331..12209
                     /locus_tag="PLT86_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT35946.1"
                     /translation="MLSAVGVLLIWSGSGVFSHLATSGWNALQAAWWESLCGSLVALL
                     FLPATGLLKQVTALQPGDFVRLTALGLLAHTGYYSFLFVGFASGHQVQTMALNYTWAV
                     FMVVFGGLINRAVPQWHQWVSVGLGVLGAILVVIPGSHSAADGTPGALAGLGAGLCFG
                     LFTPLSVRWSYDPRLVTSWMLFLSMLVFSLVMLFSGMPFNFRANGMIGALYMGIGVDF
                     VGYALWQRANRRLHPWVVSVWACLIPLLNITLLTLVAGLKMPSPVYLGGGLIVAALVV
                     QNAFALATRQTEPRGS"
     gene            12295..13389
                     /locus_tag="PLT86_04720"
     CDS             12295..13389
                     /locus_tag="PLT86_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT35947.1"
                     /translation="MNMFAGKERPHKPDWEAFVSTVARRGTPKRVHHIELYQDSEIRD
                     AIAERYGLADGLNKADPYFAQKQYIAVQRFCGFDYVVAGLTGLDMPLHQTAIPDTAAL
                     KRDGGRNFMNEHAGPITTWEEFEKYPWPDPGAPHATRLLEWYERNLPDDMCIIGGLTG
                     HFAELITWLMGYETLCYALHDDRTLVKAIADRLYEFYEADVQHLLQFERVKMVWGSDD
                     MGFKTGLLLSPDVMREYVLSGHRRLAQMVHEAGRLYLLHACGNLSSIMDDLIFDVKID
                     AKHSFEDTIEDVRQVKHTYGRHIALLGGIDLDFLCSASEERIRERVRDTLAVCQDGGG
                     YCLGTGNSVANYIPLDNYLAMVDEGMRWSR"
     gene            complement(13443..14225)
                     /locus_tag="PLT86_04725"
     CDS             complement(13443..14225)
                     /locus_tag="PLT86_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT35948.1"
                     /translation="MPIIDCHVHAGDKYGPVENVIAQMDANGVTHAVIVQHMGQTNNT
                     YLRQCIDKYPQRFWGICMVDLTLSNAPKILRSELKENGLHGVRLPAMSLSRRPDLWEM
                     AVECKAVISVLGLQPHDLGQVERLRAFLEKYPTARLKIEHLGYPEPSEPPPYATYQRV
                     MDLSQFKGVFLQFSSPYTQSKKAYPYDDLLPFIEMAKQHFAPNRIVWGSCYPPVEKYM
                     TYAQSLAWLGTLGLKDTHRDNALWQNAVRLMDPRKKKVVAET"
     gene            complement(14464..15384)
                     /locus_tag="PLT86_04730"
     CDS             complement(14464..15384)
                     /locus_tag="PLT86_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOT35949.1"
                     /translation="MRVDYDVVDNKLVQTNKDDALATVFVNPDAQERTYLTETLEIDE
                     HTLTSALDPDELPRLEFEPEHTVLIFKNPKNYSATDELLLKAMSVGVFLFKDRVVVVA
                     SEDIPSLNIARFARVRTATDFLLYLLYRSIFHFREHLKVINMISDNLQDEIKSSMDNR
                     KLIDLFTLQKSMVYYLYAIQGNGALMDKLKSNAAKIRLSVEQLESLDDIIVENNQCFR
                     QAEIYANILASLMDARASIVSNNLNVLMKTLNLITIFIMLPTFVVSAFSMNVDLPWNL
                     KQQPYAFWIIMGLAFLSIVGGFIFWRHKKW"
     gene            complement(15928..16533)
                     /locus_tag="PLT86_04735"
     CDS             complement(15928..16533)
                     /locus_tag="PLT86_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35950.1"
                     /translation="MNSVNPSHFSLRQEILRKLIHLMGLVLPLGYSQVGRSRGLPLLA
                     LATVMVVTADALRLYWPFAGRVYRRIFGGLTRSGENSRMTGASYMMVGQALTAALFPV
                     PVAIVAMTFGAVGDSAAAIGGRLSGKHLWLRGKSIEGSLACLIACFVAGTAWAELPLA
                     CVWTGAVVATLAEALPLRVNDNAIIPVSSGAAMAGLMFLFM"
     gene            complement(16530..17936)
                     /locus_tag="PLT86_04740"
     CDS             complement(16530..17936)
                     /locus_tag="PLT86_04740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOT35951.1"
                     /translation="MEKTDPLLGAIGAVRERHIVHNLRRIFEIIASVERGDSTLEKTA
                     QSYFRSHPKLGSSDRALIGDVVITTYRWKMRLQNALRHTLVQERPTGFRAEETDALLP
                     GAVLISSGYDANVVTSAYRRVFPSLDLPPLFWEQLASHVFSLNPWESADLEQLALRAS
                     LPEWVVRRLTADHGFERARSLAIALQTKAPITLRNNDLRQDRETLIAELLAAGVAVVP
                     TVFSPWGVQLSERREVRHLQGFGSGAFDVQDEGSQLVAFATGAAPGMVIVDACAGAGG
                     KSLALGAMMRNEGTIAAFDPSEAKLEELRVRAARAGLRNVQTHRVPATPEEVPHEAVF
                     QRLVGHADVVLVDAPCSGSGTARRNPDIFARITPEGVTRYSQTQRRLLETYSRLVRPR
                     GRLVYATCSLFREENREVAEGWLRDAADFISIPVERSIPSEIAPSVCEGAYLSVFPDT
                     HGTDGFFVAAFERKSEPQ"
     gene            complement(18102..18800)
                     /locus_tag="PLT86_04745"
     CDS             complement(18102..18800)
                     /locus_tag="PLT86_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOT35952.1"
                     /translation="MEKEPEGRVEFLQRVCGAAARRAPERPTSGPSKITGEVGRSVRD
                     GQLADIFVGNARAAGIDARIVSTRTMVVDAVNDITRTYSVRTLHAWNDGTVERYGLES
                     LAEMSGLEIQRWNPEQPEESRKAAAFGMQCGLTSADYAIAETGSLVLTTSREKGRSVS
                     LLGDVHIAIVETDRLIPDLYDLPQRLLKDHPALLPSNVTIISGPSKTADIELQMVVGV
                     HGPRHVFAILVQSD"
     gene            complement(18815..>19159)
                     /locus_tag="PLT86_04750"
     CDS             complement(18815..>19159)
                     /locus_tag="PLT86_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT35953.1"
                     /translation="DSLTGFHKPDTRLFEWALTRANCRAANAVMVGNRLDADVIPAKR
                     SGMKAILCEIELSTKGWYPPGGDELLYLQSLSRVPNWRNNTAELSAGDQPDGFITRLS
                     ELPGVVEKLLAE"
BASE COUNT         3870 a         5472 c         5547 g         4270 t
ORIGIN      
        1 gctttttcat ttctctgcgc tccttttcgg cgtttcagtt ttccggctgc gcaaaacgca
       61 tgtccacgca cacggggaaa tggtccgacg ggtatttgcc gtctcgttca aacgcgaccg
      121 tctcatacct ggtgacctgg ccctctcccg cgagcagtac ccagtcaatg cgacgacttt
      181 ccgggagcat gccgcctctg aatccgtgta ccgtccctgc cggaccaaga acctcctgcg
      241 caagaacacg cgtgtcccga aagccttctc cgatgccgcc gccggaacgc gtaaaaaaac
      301 cgtgaaggcc gccgtctggt ggagcgttga aatctcccat caatacaact ggcgcagact
      361 tgggtaccaa agccaactga ctttccacga ctcgtgcgca cgcaagctga atgtcctcat
      421 cccttgtcag cgggaaatga gtgttcagaa ccgaaaagcg aaacccgtcc gcgatatcca
      481 tcagagtcgc cgacgtaacg attcgagggt gatccgaagg gcaaaacgtg cgtgtgcccg
      541 gcacgtccgg cgtgtcagag aaccattttg tggtgctgcc ttctgcaagg aacctctcgc
      601 gtctgaaaaa caccggattg tgttcctcat ccgttccccc gtaccgctcc ttcccaactg
      661 ccgcgtaacc cggaagcgca ctcagcaggt cattcagttg tcgccggtac acctcctgca
      721 tgccgatcac gtctggatcg tacaactgga tcatttcgaa cacgcgggca cgcctctcgt
      781 cccagagatt cccggtgtcg gactcacagg ggaaacggag gttgaagctc atgaatcgta
      841 tgtctgcgaa ctgattgtgc atagtcccgt cctcacgcga aataccacat ccctgccgcc
      901 ccgactatcc agcagtagac tgcgaaccag tgaagcgcgc ctttcttgac aaatgacacc
      961 aggactcgga cggccgcgta acccgaaatg gccgaagtac agaaccctgc aatcagcggc
     1021 ccgctcaaca aagccagccc gccgggttgt ccgaccatgc cggcgacgtg gatgatggcc
     1081 cctccaagaa tcacagggat tgaaagcagg aacgagaact ccgccgcctt ttcgcgttca
     1141 attctgagaa acaaacccat tgaaatcgtt gaaccggaac gcgatatgcc gggaagaatc
     1201 gccagcgcct gtgcgaaccc cactgccagt gcgggtgata gcccccaatc gcggtcgttt
     1261 cgcctgacaa aacgtgtcgc gaaaagaata atccccgttc ctatgagcag cacggaggtc
     1321 tggaaaggat ttccgaaggt ttccttcagc gagtcctcga agaggacccc cagcagagct
     1381 gcgggaaccg ttccggcaat tatgtacgtt gcccggcgaa actcctctcg cgcccacggg
     1441 gaacgccgaa ctgccagatc cgcaaccccc gctattgtac ctctgatgat cggtgcgatg
     1501 cggtaccaca tggcaccgaa aaccgcgaca agggttccca aatgaaccac gatcgcaagg
     1561 tcgacatttc cctccttgat gccgaggaaa tgctctgcgt acacgatgtg cgccgaactg
     1621 gaaaccggaa gaaactccgt cattccctgt atcacaccta caatagcggc ttcagaaacg
     1681 ctcatggaaa gctccaaaat ggaggacggc aggtacgatt cagtgcgcgt cacctcgccg
     1741 catgatcatc gcgaccgtcc gtcccgtagt gccgccgttg gcacggtggg aaaaaaacag
     1801 atcgtggcgg cacactgtgc acgttgaaga gatgtctatg tttttcgccg cgatgcctga
     1861 gtccgctagc tgacacaccg caacggcgga caaatcaacg tacgacttgc cgccgcgtgc
     1921 gtgcggcgca actacatgtt cttttcgaaa ctgatgggcg acgtcgtctc ccacctcgaa
     1981 acagcaggcg cggatgtggg gcccgatcgc cgcacgcatg gattccggtc gggcgcctga
     2041 taccgtctgc atagccgtga cggttcgcgg gattacttcc gcgagcacgc ctttccatcc
     2101 cgcgtgaatt gacgccacga tccgtccgtc ctcggacgcc agcaggaccg gaaggcaatc
     2161 cgcggttccc accagcacgg gtatggcttc gcgcgtagcg atgaaagcgt cagcacggcg
     2221 tgctgcctcg ggactcacac tatcgcacaa caccacatct gagccatgct gttgcgcttg
     2281 cagggttacc atttccggtg agattccggc ggatcttgcg agcaacgacc tgttctgcag
     2341 aaccgcatcg ggggaatccc cgacgttcaa cgaaaggttg agaaacgcga acgagcccgc
     2401 actcacgccg ccgattcttg tcgtgaatcc gtgccatacg ccaggcaact cgtccagcag
     2461 cgatgaacgg agcattggca ggccggtttc tgaagtaagg cggaacacgc tactcgtcct
     2521 cgtcgtccgg tgattcctcg acaaacggca acggacttcc ggcgatcatc gcgcgcagtt
     2581 cgtcttcgtc gtcgccttca gtcgccagtc ggtgaacctc taccacgttc ggcactttcc
     2641 ggagtttcga aatcactcgt ttgagctgct tgaggttctt cacttccagc aggaacgcgc
     2701 tgtgagcccc gtcgggcaga gtgcgaattt ccgcgtgccg tatgttcgcg cccgtgctcg
     2761 caatcgtgct tgtcacgttg ctcaggagcc ctactctgtc agaaccttta acgatgattg
     2821 ccgtcttgaa aggacccgcg cccccgatga catccttcgc ttcccaccga actggtatga
     2881 ggcgtcccgc gtcttctttc aactgccgga tgttggggca ctcgcggtgg tgaaccgtca
     2941 aaccccgtcc ccgggtgatg aaaccgagga cgggatctcc cgggacaggg ttgcaacatc
     3001 gcgcgatccg cacaagcggg ttatccattc cgctcaggac gatccctccc gcttctgaat
     3061 cagttgcggg gagttccttt ggaggcgctt cttcttttgt cggcgccgga gcgatcatcg
     3121 aacgtatgtg gtcaaaaacg cgttccggcg cgaggtcccc ggcacccacc gcggcgaaaa
     3181 gatgctcgcg atccttcgtg ttgaacgccg acgcaaccgc gccccactct tcctccgcga
     3241 ggacgagccc ggccttcttc agacgtttct ccacaaggtc gcgtccgatt tcgacgttga
     3301 gcgcgaattc cgcctggcga agccactgct tcacccttgt tcgggctttc gctgttttca
     3361 ccaggttgag ccagtcgcgg ctcggtttct ggttggccga cgtgatgata cggaccgaat
     3421 cagacgtctg gagctcgtac ccaagcggta caattcgccc gcccacgatc gcgccagacg
     3481 catggagccc cacctccgta tggaccgcga aggcgaaatc cagtgcggtt gcacccttcg
     3541 gcagaggtat cacatcacct ttgggagtga gagcgaatat ttcgtccgga aagaggtcga
     3601 ttttgagctg ctcgtagaac tcattcggat cccgaatatc tttcgaccac tcaaggaact
     3661 gccggaccca gcgcatctgc gcgtcgattt cccccgtagc ccgcccttct ttgtacagcc
     3721 agtggcgcgc aattccttcc tcggccacct cgtgcatttc ccgtgtacgg atctggactt
     3781 ctaccaccgt tcgctggttg ttcgtaacgg ttgtgtgaag cgactggtat ccgttcgatt
     3841 tcgggttgcc gatgtagtcc ttgaatcgcc cttcgagcgg gcgatagtgc tggtggagat
     3901 atgcgagcgc gtcgtaacac tgatccgcgg tttcgacgat aatgcggact cccagcaaat
     3961 cgtagatttc ggacagatcc ttgccttccg aaaccatttt attgtggatg ctgtacaggt
     4021 gtttgacccg cccgctgact tccgccggca taccgcgttt tgataagcct tcccggattt
     4081 cctcgcagaa ctgctcgatc gcattccaat attcgtcttt gcgctgcgcg acctgcgcct
     4141 caagacgggc gcaggcttcg ggatcggcat agcggaacgc aagctcctca agctcgcgac
     4201 gaacgcgtgc cataccgaag cgatgcgcga ggggagcgta gacgtccagc gtttcccggg
     4261 cgatccgcag gcgcttttca ggaggcagat aatcgagcgt tctcaggttg tgaagcctgt
     4321 ccgcgaattt tacgagaatg actcgcacgt cctttgcgac ggcgagcagc atcttccgga
     4381 aattgtctat ctggttttcc ttgtcgctga cgagctgcat cctggagatc ttggtgaccc
     4441 cgtccacaat cgtggcgaca tcattgccga aattcacctc gatttcttct atcggggttt
     4501 tcagatcgtc ttcaacgacg tcgtgcagca gaccggctgc aagagtttgt gtatcgagat
     4561 ggagatcgac aaggatgcga gccacgtgca ccggatgcac aagatagggc tcgccggaag
     4621 agcggaaatg tcctttgtgg cgttcggaag cgaagcggta cgctctcctc agggtcggca
     4681 aatcgatgtt tttgtcgaag cgtgaagcca gttgcatgat ctccgcaaac aggcggtcgc
     4741 actgtgcagt ttccgcacga gctttttcgg tttcttcggg cacgttcatc gcagatccaa
     4801 tgcgtccgcc ctgcggtctg gtatccttat ccggcaattg atttgccgaa cggcaggtgc
     4861 acctgtgcgc tgccacagcg cgcattaaag atagcgcaag aaccatattg ccggaactct
     4921 gaggccttcc gttttgtctt cccattcaga agcaacgagg aaccgccggt ttcgcccggt
     4981 tgttgttcgt tacgtgtgag cgagcgtttg gcgcccagag tgcgcatcgg tatatttgtt
     5041 cggtgacata accagtgcgg aatcgcaccg gttgatacca ggtttgtgga ggcaagttat
     5101 ggcagacgca ccagttgccg ttgattctct cgttactatc tccgaatcag ccgccaagaa
     5161 gatccgtgag ctgcttcagg aagaagggaa cccgaactac gcgttgcgcc ttcaggttgt
     5221 gggcggcggc tgcgcagggt ttcagtacaa gctcgcgttc gacgacgaat ttgcagaaga
     5281 tgatgagctc ctggactatg agggattccg ggttgtcgtt gaccccatga gtgcgcgtta
     5341 cgttgaaggc gccactatcg attacgtcga atctgagatg gcgtccggtt tcaaaattga
     5401 gaatcctaac gtggtcacca catgcgggtg cggacattcc cactcattct gagcattttg
     5461 ttcgcgcgca tgttcttgtt ctggcccggc gccggtaagg gtcgcttata acgccattcg
     5521 ggatatcgta tgatgaccgg atggcttttt tttgcacctg agcgacgcgt ggaggctttt
     5581 tccggaacgg gctctgagcc tggccttgtt tttgcttttt caatgctccg caacaaagtg
     5641 tccgacacac cacggctggc atggcccagc tcagtcaccg gctcaagttc gaggaattta
     5701 cgttgaattc aatcgcgcat ggtctgttcc ggcgtgtcgc cgaaaagtgg gtggcgttca
     5761 gtcttcgtca cctgtggttg actgccgccg tatcgctcgc attcaccggg cttgcgttgt
     5821 gggcggcctc attcctctct ctgaaaacag atctggccac cctgctgccc cgggattacc
     5881 cgagcgtgaa ggaactggat cgcgttcagc agaaagtcgg ggggcttgag aaggtaattt
     5941 tcgttatcga gtcccctgat tcggccgcca atcaccggtt cgcacttgcg ctgggccaga
     6001 aactgctggc ggactccgca tacagctatg tcgaatggag ccgcgacctc agcttcttcg
     6061 acgaccacaa actcctctac atggatcttg aagacctggg ggaggtctac cgtcgcgttt
     6121 cgtccaaggt tcaaatggat gagttcggcg agccactcga cttctcggat atcgaggaga
     6181 agtacaaggg aacgaacggt tccgggaaca actcgaacgg ttctgacgga tgggcaaccg
     6241 cagacggcac aacacggatc ctgattgcat atccggcggg tcaatccggt gacgtcggtt
     6301 tttcacggaa ggtgatcgct tccgccagaa agcaagtcgc agccttgaac ccggcgtcgt
     6361 tccacccgca gctcgaagtg tactacggcg gcgagttcaa gaatcgcgtg gacgaatatg
     6421 agtctctggt gagcgatatc aaatccaccg cgatcatctc gattctcggt atcatcatca
     6481 cgatctcgct gtacttccgg cagtattttg cgtggctcta catcgggtac ccgttgatcg
     6541 cgggcctcgg gtggacgttc gggattaccg ccctcaccgt cgggcacctg aacctgatca
     6601 ctgctttcct gatagccgcg ctgacgggtc tcggtatcga tttcggcatc cacgtgttcc
     6661 aacggtacac cgaggaaagg gggcgcgggc aggaccttga aacggcgacg acgcgtgcga
     6721 tcgtcaacac aggttccgct cttctgacca gtgcgacgac taccgtagtt gccttttacg
     6781 cgctcacaat aacggatttc aaagggttct ccgagtacgg ttttatagcg ggaaccggag
     6841 ttctgatgac gctcactgcc gtgcttttca gttttcccgc gttcattgcg atcggcgagc
     6901 gcctgaacct cgtgcggcac cggcacgctg cgaaaaaatg ggcggaatct acggcgacct
     6961 tcccgtttgc gaaagcggtc gtgtatggca cggtgttcct tctggcgatt gcggtgtttg
     7021 ccgcggcgtt ctacccgcga gtgcgatttg actacgattt ctccaacctg cgttcgaaca
     7081 tccctgcgtc gaatctcgtg aaatcgaaga tttccaagat acaaccgatg gaatccgctc
     7141 ctgcagcgat tgttgtcgaa gactcggccg cgcggatgga agtggcagac gctctgcgtg
     7201 caatccagcg agccgacaca ctgagtccga cgatcaagga agtgcgtacc ctggaagggt
     7261 tcgttccggc ggaccaggac cagaagatgg cgttgatcag gcggttgcgc cgcgttgttg
     7321 aaggacatga ggaagccatt ctcgaagggc aatccaacat caatgttgag gggctcaaac
     7381 gctggttgaa cgtgaatgaa atcacgacgt cggaccttcc ggacttcatt gtccggaaat
     7441 tccgcgggcc cgatgggagc ctcggcgaat tcgttgtcat aggtacttcg gtttcgcttc
     7501 gcgacggcaa gcagacgatt gccttcgcgg aagatatccg ggaaatcgac acgcctcatt
     7561 tcgggaagtt ctacgcatcg ggcagcgcgg tgattttcgc agacatgttg ctggtgatgc
     7621 agaaggacag cgtgatcgcg accaccgtga cgctgctggt ggtgttcgcc atcgtgctgc
     7681 tgcaatttgg aacgaaacgc gccgcattgc ttgcgatcct gccggttctg gtggacggtg
     7741 tgctggttgc cttctcgaaa atcacgggag ccggcttcca cgcgaagttt gaggaactgg
     7801 cgtgggtgtt tggcgcggaa gcagttctgg gtgctctcct cgtgtttctg ttcgagcgca
     7861 aggcgttcaa aaccacgatc ttggtcatgg cgccgctcct cggtggtgtc attctaatga
     7921 tcggaatcat ggcgctgacc ggtgtgcgtt tcaatttcta caacattgta gctctgccca
     7981 gcattctcgg catgggcgtg gacaacggcg tccattttta tgcccgttat cgagaagaag
     8041 ggcgtgggtc cgtgttgtac gtcctcagga ctaccggggc ggcggcgctg atggcttcgc
     8101 ttacgactat ggaaggtttc ggcgggatgt tgacggcaga ccatcggggc ctctactccc
     8161 tcggtctcat ctcgataatc ggcatgggaa cgtgtttgta tatgtcagtt gtcttcctgc
     8221 ccgcttggtt cgcgtaccgt gaggagcgac acaggaagac ggactgagcg gacctcgtgg
     8281 catcgcggga aaaaggcatg ctggttcctt ctgcagatat ccggcatctg catctcatcg
     8341 gcatttgtgg tacggcaatg ggtgccttgg ccgtgctcct gaaagagcgc gggttccggg
     8401 taaccggttc cgacaacgcg gtctatcctc cgatgagcga ggtgctgcgt gaccatgaca
     8461 tccccgtgat gtcggcgtac tcgcgggaca acatcacgcc tccaccggac attgttgtca
     8521 tcgggaatgc cctgagccgc ggaaatgagg aagtcgaagc ggttctcgaa cgtaagatac
     8581 cctttgtatc gctgccggag gcgttgcgcg aattcttcat tcgtgggtca acctccatcg
     8641 tcgttaccgg cacacacggc aaaacaacca caagttccct tcttgcgtgg attctggatt
     8701 tcgcagggtt gcaaccgggc ttcatgatag ggggtgtgcc agcgaacttt ggcgtgggcg
     8761 cgcgcgctgg atccgggcgc ttctttgtca gtgaaggcga cgagtacgat accgcgtttt
     8821 tcgacaaacg atcgaaattc ctgcattacc ttcctgatat cgtcatcgtc aataacatcg
     8881 aattcgacca cgcggatatt ttcgcggaca tcggcgagat agaactgagc ttccggagac
     8941 ttctcaacat cattccccga aatggcctcg tggtggcaaa cagggatgac tcacgggttg
     9001 tccggcttac ggaacacacg tggccgccga aggtgacgtt ttcgctttca ggagacgcca
     9061 actggcacgc gcgcaacgtg tgcgataccg cgtgcggcac tacgttcgat ctcgttggac
     9121 cctgtgggca gataacctgt gagttaccgc ttttcgggcg tcacaatgtc aggaatgcgc
     9181 tggcggttgt cgcggtgtgc gcgcacgttg gggtctcact ggagcagata cgagacggca
     9241 tgcggagctt caagggggtg agaagaaggc ttgagaaact tttcgacgcg caccagattg
     9301 ttctttacga cgatttcgcg caccacccga cagcgatcac ggagaccctg cgcggtgtca
     9361 gagcagcaca ttccggatca cgcgtctggg cggttttcga gccgaggtcg aatacgacgg
     9421 tacggcggtt ttttcagact gaacttgcgg atgcgctggc atgcgcggac gttgtgcgac
     9481 tgggagcagt acacagaaac gacagaatcc ccgcaagcga acgacttgac gtggcccggc
     9541 tcgtgtccga ccttcgcgaa cggggggcgg acgcggaatc ggttccagat gtcagcagac
     9601 tgagtgagga aatcgcgtcc cacgcccgtg cgggtgacgt tatcgttgtc atgagcaacg
     9661 gcggattcgg ggggctgacg ggattgcttc gtgacgcgtt ggaaaagcga tttggtgcat
     9721 cagaatctgc gacgtagagg tgtgaaatgc tcagctcacc caactggact atcgccgaac
     9781 ggcacatggt gcggtaccat atgtcccacc tcatgtggtg ggcgctgatc gcgataagtg
     9841 tgcccgtaat cgcgctgagt ctcggtgccg cgcttgtcaa gatcgctgtt cttccgccgc
     9901 gtggggcagg gtttctcatc tggggggcta ccgcagcgat cgccgttctc tccccggtgg
     9961 tggcctttgc gatcgaacgc ggccgcatcc gacccgtacc cgccgaccgt ccgcttcggg
    10021 cggtggtgat gtggtacaag cacgcaaaac tgtgggcgat cgctgcgtgc ggaatcgggg
    10081 cgctgtcgcc tgtttctgcg ctgtatatct ccggttcttt ctcccccgcg cttggcgctg
    10141 ttgtgtttcc tctggcattt ctgatgattc gtttccccca cgcagagcgt ttcaccgcgt
    10201 tcgtggacag ccactttgac gcagtggaac cgtcgcaggg accagaatga aagtcgttgc
    10261 cgtcaggcga cctccgatgc cccggtctcc tttgtcgtga gctcctcgcc gttcattgcc
    10321 atctgcaggc ccattttcct cctgatttcc gcgaagactg cagtccagcc gattgctcca
    10381 cggcatcccc gtgctgagat ttgaaacgga acgatattcg gttttgccca agaccagggg
    10441 agattccgtt tgcgtacgat tctactggcg gcaattgcgg ccgcgtgtgt ggtatgtgca
    10501 tggtcgcaag cctctgcggc agtcatagtg acgacgagcg gagaggagat tgtctgcgag
    10561 attgtgcgcg aaacggacac gtatttcgtc gtcgaccacc gcggttaccg gagatacgtg
    10621 ctgaagacac aggttcggtc gctggatcgg agagcagggg aggggttcaa tctcggtccc
    10681 gagccgttga ggaacatcga gttttccggc gggtaccgcc tgtgggggcg gtcacaaagc
    10741 catgggattg gcgcttcagc gatttctgcc agttaccctc tgaacgaaaa ccttcttgtc
    10801 caggtgggcg ttgaggcggg ccggggcgaa gcatcgtcct cgttcgaggg cggcgacctg
    10861 ctcccggggc cgatatcgtg ggtcgggggc atgtgtggcg cacagtggca attgcagccg
    10921 tggcgggtac ggcctttcct cgatgccgga atagggtact tcgcgatgcg ccattccgtt
    10981 gctgattctg gggtgctctg gatgaaacag gccatcgccg ctatcgacgg cgatacgagt
    11041 ttcgtgtact ctgagcccat ggatggtgcc ttcgccgtca ggcttggagt gggcgccctc
    11101 gtgcctttgt caggacggtt tgcgcttacc ctgcgcacgt ccattctcgt gctgcatacg
    11161 ggtttcacgc gccagtggta ctatcccacc tctgccgaac aagaggagat gacgctgcga
    11221 tcatcccacg gaatcacact ggagatgatc cacacgaccg caggcgttca atgggcattt
    11281 tgaactccga cgggggggta tcccgtgttt ccgtatcccg accggaggcc gtgttatcag
    11341 cggtcggagt gcttctgatc tggagtggtt ccggcgtttt ttcccatctt gccaccagcg
    11401 gatggaacgc attgcaggcg gcatggtggg aatcgctctg cggttcactc gtcgcccttc
    11461 ttttcctccc ggcaaccggt ttgctcaaac aggtgacggc cctgcaaccg ggggattttg
    11521 tgcggctgac cgcgctggga ttgcttgcgc acacgggtta ctactcgttc ttgtttgttg
    11581 gctttgcgtc agggcaccag gtccaaacga tggccttgaa ctacacctgg gcagtcttca
    11641 tggttgtgtt cggcgggctg atcaaccggg cagtgccgca gtggcaccaa tgggtatccg
    11701 tggggctcgg ggttctgggc gcaatactgg tggtgattcc gggttctcac agcgcagcgg
    11761 acgggactcc tggagctctg gcggggctgg gtgcggggct ttgtttcggg ctcttcacgc
    11821 cgttatccgt gagatggtcg tatgatccgc gtcttgtcac gtcatggatg ctatttctca
    11881 gcatgctcgt gttttcgctt gtgatgcttt tttcggggat gccgttcaat ttccgggcaa
    11941 acggaatgat cggcgcgctt tacatgggta tcggggtgga cttcgtggga tatgcgctgt
    12001 ggcaacgcgc gaaccgtcgt ctgcatccgt gggttgtttc cgtgtgggct tgtctcattc
    12061 cgctgttgaa catcacgctg ctcacgctgg tagccggttt gaaaatgccg tctccggtgt
    12121 acctgggcgg tggtctgatc gtggccgcgc tggtcgtgca gaacgccttc gcgctcgcga
    12181 cgcggcagac tgagccgcgg ggcagttgag ttgacgcctg cgcgacgaac gatgatacaa
    12241 cgatgatgga cggtttattt tcgggatccg ccctttttga tcgaggatga aacgatgaac
    12301 atgttcgcag ggaaggagcg cccgcacaaa cctgactggg aggcgtttgt ttcgaccgtt
    12361 gcaaggcgag gtacgccgaa acgggtccac catatcgagc tttaccagga cagcgaaata
    12421 cgcgatgcga tcgcagagcg ctatgggctc gcggatggtc tcaacaaagc ggacccgtac
    12481 ttcgcgcaga agcagtacat cgcggtgcag cgattctgcg ggtttgacta cgtggtggcg
    12541 gggttgacgg gcctcgatat gccgcttcat cagaccgcca ttccggacac ggcggcgctg
    12601 aaacgcgatg gcggaaggaa tttcatgaac gaacacgcag gcccgattac cacatgggag
    12661 gagttcgaga aatacccctg gcctgatcct ggcgcgccac atgcaactcg tcttctcgag
    12721 tggtacgaga ggaaccttcc ggacgacatg tgtatcatcg gcggtttgac cggccatttt
    12781 gccgaactga taacctggtt gatgggctat gagactctct gttacgccct gcacgacgac
    12841 agaactctcg tgaaggcgat tgccgaccga ctgtacgagt tctatgaagc agacgtccag
    12901 cacctccttc aattcgaaag agtgaagatg gtgtggggaa gcgacgacat ggggttcaaa
    12961 accggccttc tcctcagccc ggacgtaatg cgcgagtacg tgctttctgg acaccggagg
    13021 ctcgcgcaga tggtgcacga ggcgggaagg ctctacttgc ttcacgcgtg cgggaatctt
    13081 tcgtcgatca tggatgatct gatcttcgac gtgaaaatcg atgccaagca cagtttcgaa
    13141 gatactatcg aggacgtccg gcaggtgaag cacacgtatg gccgccacat cgcgctcctc
    13201 ggaggtatcg acctcgattt cctttgctct gcttctgagg agcggattcg tgagcgggtg
    13261 agggatacgc ttgccgtctg tcaggatggc ggagggtact gtctgggcac agggaacagc
    13321 gttgccaatt atatccccct cgacaattac ctcgcgatgg tggacgaagg tatgcgttgg
    13381 agccggtgaa gaagaaaaaa gggcgcgtaa aacgcgcccc ttttttgatc tgcaagctcg
    13441 tctcaggttt cggccaccac ctttttcttc cgtgggtcca tcaaccgcac agcattctgc
    13501 cacaatgcgt tatccctgtg cgtgtctttc aatccgagtg ttccaagcca ggcaagcgac
    13561 tgggcgtacg tcatgtattt ctcaacgggc ggatagcatg aaccccagac gatgcggttt
    13621 ggtgcgaagt gctgctttgc catttcgatg aaaggaagaa gatcgtcgta gggatacgcc
    13681 tttttgctct gtgtataagg cgaggaaaac tgaaggaata ctcccttgaa ttgcgacaaa
    13741 tccatcactc gctggtacgt cgcgtacggc ggtggttcgc tcggttccgg gtaccccagg
    13801 tgttcaatct tcagccttgc ggtagggtat ttctcgagga acgccctcag ccgctccacc
    13861 tgccccagat catgaggctg aagtcccagc acgctgatca cggctttgca ctcaaccgcc
    13921 atttcccaca gatccggcct acgcgaaagg ctcatcgcgg gcaacctcac accgtgcaag
    13981 ccgttctcct tcagctccga cctcaggatt ttcggagcgt tcgaaagagt gagatcaacc
    14041 atgcagatgc cccagaatcg ctgagggtat ttgtcgatgc attgtctaag atacgtgttg
    14101 ttcgtctggc ccatgtgctg gacgatcacc gcgtgggtta cgccgtttgc gtccatctgg
    14161 gcgatgacgt tttcgacggg gccgtatttg tcgcccgcgt gtacgtgaca atcgatgatc
    14221 ggcatagaaa accctttgca cacgcaacag aaacgcgata ctgcgcttcc tgtgttacga
    14281 cgattggtgc tgtgaaccgg acttcagggg tgcccgcagg cggcgtcgtg gcaggcgttg
    14341 ctcctcgaac gacaaccgca tcctgaggta attataagaa gtgcagagca gtatgagaag
    14401 tggggcggtg acggcgggtt tggaatcgtg gcagacggtc tgtcgaaaaa gagaactgga
    14461 aagtcaccat ttcttgtgac gccagaaaat gaaaccacct acgatcgaga ggaacgccaa
    14521 acccatgatg atccagaacg cgtatggctg ctgcttcagg ttccacggca ggtccacgtt
    14581 catcgaaaac gcgctcacga caaacgttgg cagcatgatg aaaatcgtga tcaggttgag
    14641 cgtcttcata aggacgttca ggttgttgct tacaatggaa gccctcgcat ccatcaggct
    14701 ggcgaggatg ttggcataga tttccgcctg tcggaaacac tggttgttct cgacgataat
    14761 gtcgtccaag gattcaagct gttcaacgct cagtcggatc ttcgcggcat tgctcttaag
    14821 tttgtccatc aacgcgccgt tgccctggat cgcgtacagg taatacacca tgcttttctg
    14881 cagtgtgaac agatcgatca gctttcgatt gtccatcgat gacttgatct cgtcctgaag
    14941 gttgtctgaa atcatgttga tcaccttcag gtgttcgcgg aaatgaaaga tggaccggta
    15001 gagcaggtac aggaggaaat ctgtcgccgt tctgacgcgc gcaaaacgcg caatgttaag
    15061 tgatgggatg tcctccgacg ccacaacaac tacgcggtct ttgaaaagaa agacaccgac
    15121 cgacattgcc ttcagtagca gctcatccgt ggccgagtaa ttcttggggt tcttgaagat
    15181 caggacggtg tgttccggct cgaactcgag acgcggcaac tcatcggggt caagcgcgga
    15241 tgtgagggta tgctcgtcaa tctcgagcgt ttctgtgagg taggttcgtt cttgcgcgtc
    15301 aggattcacg aaaacggtgg caagcgcgtc gtctttgttt gtttgtacca gcttgttgtc
    15361 gacgacgtcg taatccaccc gcatactgac gctccatgac ctctaaagct gtttcaagcg
    15421 gcttccgcac accgcgaacc cggaagagat acttctgaaa attacgcagt acccgcgccc
    15481 tgaaaccagt cttgttgtct gcggaggacg aaaaaagccg cgggctcttc gcaagaacct
    15541 aggcaccgca accgttttcc gcgacgaccc agaccgcagt cccccggttg ccggtatccg
    15601 atcgaaacag ggtcgacccc ggtccggatt tttcgggtga aggactcaaa caggcaatga
    15661 gacaggcgtg cggcgtgttg gtcgcattgg aaaggatcaa tgttccccgc aggctcctca
    15721 ccgccgcgag caggttgccc aaaacgacca cggcgtgagc gcattcccct cgggcaccgg
    15781 ttcgttgcgc ttgccgcgtc ggtggaataa agggcggacg cacccccgca aatcgcgcga
    15841 aagggccgcg atccgcaggg gatcgtcttc gcaattttaa tatcgggtgg agaattgtcg
    15901 tccgggtgcc ggagaggatt cagaagctca catgaagaga aacatcaggc ccgccattgc
    15961 cgcaccggat gataccggaa tgatcgcgtt atcattcacg cgaaggggca gggcttccgc
    16021 caacgtcgcg acaaccgcac cggtccagac gcacgcaagc ggtaattctg cccatgctgt
    16081 gcccgcgacg aaacacgcaa tcagacacgc gagggaccct tcaatcgatt tcccgcggag
    16141 ccacaggtgc ttaccggaaa gcctgccccc aatcgccgcg gcgctgtcac cgactgcccc
    16201 gaacgtcatg gcgacaatcg ctacgggaac cgggaacagt gcggcagtga gtgcctgccc
    16261 gaccatcatg taacttgccc ccgtcattct cgagttttcg ccggaacgag tcagcccgcc
    16321 gaatatgcga cggtagacgc gaccggcgaa tggccagtac agcctcaacg cgtccgcggt
    16381 gacaaccatc acagtcgcaa gagcaagcag aggcagacca cggctcctgc caacctgtga
    16441 atacccgagc ggcagaacca gacccattag gtggatcagt ttgcgcagaa tctcctgccg
    16501 aagcgaaaag tgcgatggat tcacgctatt cattgtggtt ccgattttct ctcaaacgcc
    16561 gcaacaaaga atccgtcggt gccgtgggtg tcggggaaca cgctcaggta tgcgccctca
    16621 cagacagacg gagcaatctc agacgggata ctgcgttcca cgggtatgga gatgaagtcc
    16681 gctgcatccc gaagccagcc ctcggctact tcccggttct cctctcggaa aaggctgcac
    16741 gtcgcgtaca cgagacgacc acgcgggcga acgagacgcg agtatgtctc cagtagccta
    16801 cgttgcgttt gcgagtaccg cgtgactcct tccggagtga ttcgcgcaaa aatgtccggg
    16861 ttccgccttg ccgttccgct tccggaacac ggtgcatcca caaggaccac atcggcatgc
    16921 ccgaccaggc gctggaacac tgcctcgtgc ggtacttcct caggagttgc cggaaccctg
    16981 tgggtctgca cgttcctcag tccggcacgt gccgctctca ctctcagttc ttccagtttt
    17041 gcctcgctgg ggtcgaatgc cgcgatcgtg ccttcgttcc gcatcattgc tcccaacgca
    17101 agggatttcc ctccggcgcc cgcgcatgca tccactatca ccattcccgg agctgcaccg
    17161 gtggcaaacg cgacgagctg gctgccttca tcctgaacgt caaacgctcc ggaaccaaac
    17221 ccctgcaggt gacgcacttc tcttctctct gaaagctgga cgccccaggg gctgaacacg
    17281 gtaggcacaa cagcgactcc cgcggcgagg agctccgcga tcagcgtttc tctgtcttgc
    17341 ctcaagtcat tgttacgcaa cgtgatgggt gctttcgtct gaagcgcgat cgcaagagac
    17401 cttgcccgtt cgaagccatg atccgccgtg agtctccgta ctacccattc tggcagcgaa
    17461 gcccgtaagg ccagctgttc caggtccgcg ctttcccagg gattcagcga gaaaacgtgg
    17521 gaagcgagct gttcccagaa caggggagga agatcgagtg aagggaagac acggcgataa
    17581 gcggaggtta ccacgttggc atcatatccc gacgaaatga gcaccgcccc gggaagcaac
    17641 gcgtcagttt cttctgcccg gaatccagtt ggacgttcct gcacaagtgt atgccgcaga
    17701 gcgttttgca agcgcatttt ccaacggtac gttgtaatga caacgtcacc aatcagtgcc
    17761 cgatcagaac tgcccagctt cgggtgtgaa cggaaatacg actgagcagt tttttcaaga
    17821 gtggagtcgc cgcgttccac actcgcaatg atctcgaaga tgcgccggag gttgtggact
    17881 atatgccgtt cgcggacggc gccaattgct cccaagaggg gatcggtctt ctccatcaag
    17941 agcctcgtga tgtcacgcgc aggacgaaag cactgccttc acgcccaaag caagatacgt
    18001 ggagggaaag aagacaacat gcggtccgcg ccgagagcta ctggacccgc atcccgacca
    18061 cgaccagctt cgcgcgcccg cgctggcagc ggatgcaatg atcaatcact ctgaacgagg
    18121 attgcgaaca cgtgccgcgg accgtgtact cctacgacca tctgcagctc tatgtctgcg
    18181 gtcttagaag gtcctgaaat gattgtcaca ttcgaaggca acagggcagg gtgatctttc
    18241 agaaggcgtt gcggcaggtc atagagatca ggtatcaatc tgtccgtttc gacaatcgcg
    18301 atgtggacat cgcccagcag actgactgac cttcctttct cacggctggt ggtgagcaca
    18361 agggagccag tttccgcaat cgcgtagtct gcagaggtca gcccgcattg catcccaaat
    18421 gcggcggctt tcctcgattc ctccggttgt tcggggttcc accgctggat ttccagcccc
    18481 gacatctctg caagagactc caggccgtac cgttccacgg ttccgtcgtt ccacgcatga
    18541 agcgttcgga cggaatacgt tcgagttatg tcgttcaccg catccactac catcgtgcgc
    18601 gtcgagacga tccgcgcgtc gatccctgcg gcacgggcat ttccgacgaa tatatccgcc
    18661 agttgcccat cgcgaacgct tcggcccact tccccggtaa ttttggaagg gccggacgtt
    18721 ggccgttcag gtgctctcct ggcagccgct ccgcagaccc gctgaaggaa ttccacacgc
    18781 ccttcgggtt ccttttccat tcctacctgt tccttcattc cgcgaggagt ttttccacca
    18841 cacctggtag ttcgctcagc cgggtgataa aaccgtcggg ttggtcacca gcactgagtt
    18901 ccgctgtgtt gttccgccag ttcggaacgc gcgagaggga ttgcaaataa agtaactcat
    18961 cgccgcccgg tggataccac cctttcgtcg aaagctcaat ctcgcaaagg attgccttca
    19021 tgcccgatct ttttgcagga atgacgtctg cgtcaaggcg attgcccacc ataacagcgt
    19081 ttgccgccct gcagttcgcc ctggtgagtg cccactcgaa caggcgtgtg tccggtttgt
    19141 gaaaaccggt caaggagtc
//
LOCUS       DAOSOO010000041        19087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2886_length_19087_cov_5.660677, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000041 DAOSOO010000000
VERSION     DAOSOO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19087
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_19087_cov_5.660677"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="PLT86_04755"
     CDS             <1..2217
                     /locus_tag="PLT86_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT35954.1"
                     /translation="GYVRERGSRESIPYVSVYIEGLRLGGMTNAAGYYVINGVPPGRH
                     VIVAAMVGYNESRQTLVVATEPLVHNIELSTEAIEVAAVEVQAERARAPSLEIIPSRE
                     TLRGVDLKIAPAAVEADPIRTLHTLPGVVQLSDFNTGLYVRGGTPDQNLILVDGSELY
                     NVSHMFGLFSTFPADAIKTTELLMGGYPAQYGGRLSSVLNVITDEGNKERFKGSGGVS
                     LLSSRMTLQGPFAKGSFLVSGRRTYLEPVLWAASRSSDAFDGMGYFFYDFQGKVHQIL
                     SHSDQFAIAGYMGKDAFKYTNDFMDTRFDWGNKSASLSWNHLFSGELYSHLQANLSRF
                     VSHTTFKIEDFGATDHNELFDTDLRGDLTWFANERHTFETGVHVKRNTMRFGQEYAGQ
                     RYEAFNLIAYVPSAYLQDSWRPWPFLTLQPGLRATLFRSRLRDTTGSATYSAFEPRFT
                     VRYQLGEQTFLKAATGRYSQFIFRVPREYEGISMMSDIWFTCDNTPGGYPQRAWHYVL
                     GLETKVRSDVDLSLEAYYKDYAGLVEFNASQTAPQSVEDALLRGDGYAYGLDVNVKKR
                     AGRNTGWLSYSMSWTVRTIDGLNLDENGNPQPFYPKFDARHHLDAVYSFDLSRHWTLN
                     SRMSLSSGQGYTKVLGRYVVEDPLFTQGLYYRGQKNGARLPYYMRLDVGAKGSYRFWG
                     VRWMPFFQIVNVLNRANEFNRYYDSGDATKNPPETGRERAIPQLPFLPTFGVDVEF"
     gene            2217..3188
                     /locus_tag="PLT86_04760"
     CDS             2217..3188
                     /locus_tag="PLT86_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35955.1"
                     /translation="MARRLWLSVALFAVVAQSGCILNPVKPEKKNVPELFLQGFLVAG
                     GPIDNIILRKTIPPEMYYDFMPLGRYAVSGAEVVITIDGAPHPLAELHPGTYGDPNLI
                     ARSGATCEIEVRFPANHEFTGRRLYGATTIPEKPRASVTIHPYYVQRGAHLNGNAVTG
                     LVFPRELSHPDSFGTTAEKEPFRLSWPLSSSATGYMVVSTAVDTQGTGLLRQRDYEEY
                     LDGVFRDPVKRQGKRTAGFYVLPDSSQSTVYWLLFSYQGWHDVSVVACDTGYWDYMVT
                     VLNDAGTGADSDAGVKLNVQGGLGIFCSYAADTTRVYIQPEWLPSTR"
     gene            complement(3249..4520)
                     /gene="serS"
                     /locus_tag="PLT86_04765"
     CDS             complement(3249..4520)
                     /gene="serS"
                     /locus_tag="PLT86_04765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT35956.1"
                     /translation="MIDLRLIREQTDFVKREIAKLFTDAPIDEILSLDEARRNLIREV
                     EELKARRNAASKAISAVKDSAERQSRILEMREVGDRIAVLDEQVRAVEERLNARLLEV
                     PNLPHPDVPVGRDESENVVTAMEGDLPTFTFEPRPHWEIGPDLGILDFERGVKISGSR
                     FYVLVGDGARLQRALITWMLDLHRTRHGYVEVYPPAVVRHDCLIGTGQLPKFGENLYH
                     DAEEDFWLVPTAEVPVTNLYAGEVLEKSQLPLYHVAYTPCFRREKMSAGKDVRGIKRG
                     HEFDKVEMVKFVEPATSDAELTSLVEHARDVCRGLALPHRIVQMCTGDLSFTAAMKYD
                     VEVWAAGCGEWLEVSSCSNFRDFQARRANIRYRPAPNARPEYVHTLNGSGLALPRVMI
                     AIMENYQLEDGSIVIPEVLRPYLGGQDTISA"
     gene            complement(4567..5646)
                     /gene="prfA"
                     /locus_tag="PLT86_04770"
     CDS             complement(4567..5646)
                     /gene="prfA"
                     /locus_tag="PLT86_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT35957.1"
                     /translation="MLDRLAAIETRFTEIERLLSDPASTTDIAQYQKLAKEHRQLAPI
                     VETGRRYADVLAQLAEARRIVEEENDRDLIAMAKEEIVVLEDRAASLEETLKEQLIPK
                     DPNDDRNTILEIRGGTGGDEAALFAADLFRMYSRYAESKGWSLEVMSTSEASAGGLKE
                     VIAQISGEGAYGRLKYESGVHRVQRVPATEAQGRIHTSTASVAVLPEADDVEVEIDPA
                     DLRIDVFRSSGPGGQSVNTADSAIRITHLPTGLVVQCQDERSQLKNKAKAMKVLRARL
                     LDRAIIQQQSEEAAARRAQVGTGERSEKIRTYNFPQGRVTDHRIKLTVHNLSEILDGN
                     LDELIEALRMADRANQLQHPSAEDD"
     gene            complement(5672..6658)
                     /locus_tag="PLT86_04775"
     CDS             complement(5672..6658)
                     /locus_tag="PLT86_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOT35958.1"
                     /translation="MESAPPADRTDSITDRTRLDLSVGGQALIEGVMIRSPRSIAIAL
                     RTPRGIAVETRDYVNLVARTRWLSLPVLRGVVSFFEMLGIGLNALTYSANVAASDGCD
                     TPVKDRISPAEALLFLISFGLGVGLFVFLPMALATLAGLTNRPVAYNLFAGTVRILVF
                     LFYLWAIGKIADVKRVFEYHGAEHKVVAAFESGLDSELTAQTARGFPRFHPRCGTSFV
                     FLVVAVAIAFYAITDSLYALYAGNAPDLETRFLLHLACLPVVAGLGYEVLKATGKSRN
                     SRRARYISKPGLLLQRLTTREPNDDQLSVAIAAVRAAVGLPQETGGPVSCAV"
     gene            complement(6671..6880)
                     /gene="rpmE"
                     /locus_tag="PLT86_04780"
     CDS             complement(6671..6880)
                     /gene="rpmE"
                     /locus_tag="PLT86_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT35959.1"
                     /translation="MKSGIHPEYHATTIACACGNVVQTSSTKKNLRVEICGACHPFFT
                     GKQKLIDTAGRVDAFRRRYNRSEAK"
     gene            complement(7010..8257)
                     /gene="rho"
                     /locus_tag="PLT86_04785"
     CDS             complement(7010..8257)
                     /gene="rho"
                     /locus_tag="PLT86_04785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT35960.1"
                     /translation="MNIIELKKKTIAELHELAHALGIPEYTSYRKQELIFKILEAETA
                     KSGFIFAEGVLEILEQGNGFLRSPDYNYLPGPDDIYVSQSQIKRFGLRTGDTVSGQIR
                     PPKENERHFALLRVEAVNFENPDLAKEKTLFDNLTPLFPNRRINLEHYPNELSTRIVN
                     LLAPIGMGQRGLIVSPPKAGKTMLLQKTANAITHNHPDIKLIVLLIDERPEEVTDMQR
                     SVDGEVISSTFDEPPNRHVQVSDMVIEKARRLVEYGHDVVILLDSITRLARAHNTVVP
                     HSGKILSGGVDANALHGPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNMELVLDRKLSDRRIFPAIDVNKSGTRREELLLTEFELHRVWILRKFLAQMSSAEA
                     MEFLIDRMHGTKTNHEFLDMMNG"
     gene            complement(8667..11354)
                     /gene="glnD"
                     /locus_tag="PLT86_04790"
     CDS             complement(8667..11354)
                     /gene="glnD"
                     /locus_tag="PLT86_04790"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOT35961.1"
                     /translation="MMTDLRQSLELLSREARAKGYSEEKSTSIQLRIFREFVERERER
                     LHYEHRAGASGHHVVQQWTAVVDAVVIEAYRAVMLNGEGTPGECALIALGGYGRGALN
                     ICSDVDLLFLFADEVPEQSPIVHGVLHLLWDVGFDLGHSARSLQSALEFASRDDIAQT
                     AVVDARFLAGLEPLFGRFVNSFRARFMADGGWEFSEKVVRQLHARREAHGSYAQVLEP
                     DVKESTGGLRDVHALQWIASARHGTASLEALVEHHLISKRDFRLLEQSIDLLWKVRNE
                     LHFVNKRKHDRLDFTAQPKVAAAFGYRDDAAALGVEHFMRDYYLAAREIVRISDAVCH
                     QALRRRRGIQTVTDIVRRRVLPDGAVYVRGKILLPKRRKGFFATNPRRMLSLFADMQR
                     FGAIMSETASWAIYDELALVDEDFRTDPENAAVFLSILGEPAHLGAVLRRMHWTGLLG
                     AFIPEFGRLTCQVQHDYYHAYTADEHSIIVIDRLSELALTDDDSRVATVYRSLSRKHL
                     LHLAALLHDVGKSGGHGHAERGAEMSITITRRLGLEKADQDLVAFLVAHHLRLSHLSQ
                     RRDIDDLAMIRAEAERFSDTDTLDMLYVLTWADMNATQARPLSRWKCQLLDTLHQRLR
                     EVVETRGLGPEFAALRTLESPQKFRNLLAERIGEEAAERHLSGMPRRYSLMHSLDEAA
                     THALRADSLKNEPVLVDVAPAGNHARVIVYTRDRRYLLSDICGVLAVNNLNILYADAY
                     TRTDGIIVDVFGVEVVGESDIRDEAFVSTLRDTFRSVWTGQAVVAELIARHRRRWARI
                     KPKAGMAPSHVVIDNTVSETNSVIDVFAIDRIGLLYDISRAISENGLDINMARIGTDG
                     DRVADAFYVVTSSGEKLIDAAESEKVRESVLRVIGNERT"
     gene            complement(11413..12228)
                     /gene="dapF"
                     /locus_tag="PLT86_04795"
     CDS             complement(11413..12228)
                     /gene="dapF"
                     /locus_tag="PLT86_04795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT35962.1"
                     /translation="MDILFTKMVGAGNDFVVIDNRNTSLPEGEPRARFVRDVCQRRLA
                     VGADGVLLLENAENAHFRMRYYNADGGEAEMCGNGGRCIARFAYLHGIAPARMRFVSA
                     AGTHEAEIRGANVKLQMTPPHSLSLNNHVELSGGTRTLHTLNTGVPHAVEVVADVDAV
                     DVVGIGREIRYHEVFRPAGTNANFITPLDRHTFRIRTYERGVEDETLACGTGTTAAAI
                     IGSFLGLFDSPVVGITQSGLPLTIHFVNRGEHAEDVFLEGNADVAFTGSVTAL"
     gene            12497..13987
                     /locus_tag="PLT86_04800"
     CDS             12497..13987
                     /locus_tag="PLT86_04800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOT35963.1"
                     /translation="MAASKRIIAIDLGASSGRTILGELGKKLSLREIHRFPNGPVSDN
                     GRIRWDFDRLWQSVQEGIRRASVDAGGDIASLGLDTWGVDYGLLGADGRLLEPPVHYR
                     DSRTNGMMEFAFKMVPRKEIYETTGLQFLQLNTLYQLLAEQMSGSGLLEKAKTLLMIP
                     DVFDYLLTGVAKAEFTDVTTTQCYDPRARGWARSMLERLGIPTHFLPEIVSPGTPLGS
                     VRPELAARLGLQGARVIAPGCHDTASAVAAVPARDGGKWAYLSSGTWSLMGVELPEPH
                     ITETALAYNFTNEGGIGGTFRFLKNISGLWLLQESLRYWREHDGEDISYAEAVARAKA
                     ARPFQCWVDPDAPDFNTPASMPEAISRYCANTGQVVPRTRDEVLRTVFESLALKYRSV
                     FDMLREMVGDIQVLHIVGGGSANELLNQFTANALGVQVVAGPVEATAIGNLMVQAMAL
                     GEVRSTAEIRTVVRDSFAVALFEPHDSADWEDAYGRWREVIAKSVA"
     gene            14152..15582
                     /locus_tag="PLT86_04805"
     CDS             14152..15582
                     /locus_tag="PLT86_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT35964.1"
                     /translation="MEATERVKGANGTVLIVDDDPAIRKLLAIALERAGFQVRQAEGY
                     RQCYGEMSDTASVDAVLLDIMLPEVNGLEILRHCSGLDSPPAIVMMTASTDVSDAVSA
                     LKLGAFDYLIKPDDTSNLPKLTGVLRNAVSFTRSKREIVRLKRELVARGRPAEIVGTS
                     APMQRVFEAIRQVADSDASVLITGESGTGKELVAKAIHEASARSRGPFVVVNCSAIPI
                     TLIESELFGHEKGAFTGAIAQKRGKFEIAHGGTIFLDEIGDMDSGSQTRLLRVLQEQA
                     FERVGGTHTINVDVRVIAATNQDLQARVRESAFRLDLFYRIAVFAISLPPLRDRTEDI
                     PLLAAHFLNLHASKAHKEATGFEPAAMNALVRHAWPGNVRELGNVIQHAVIVSEGPTI
                     RLEHLPPHLVQASVPETPAGENAVLLRDPSTHDIRPLKEVEKEVMKRALEATEGNFAL
                     AAMKLQIGRATLYRKVKEYGLDADGT"
     gene            15651..16169
                     /locus_tag="PLT86_04810"
     CDS             15651..16169
                     /locus_tag="PLT86_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35965.1"
                     /translation="MSPYVNRIRRALKLDPALFEEVEADRHATGEAVGVVLLSSGATG
                     LGAIPQTSIGSLAILFLAVLVGWCAWVYVTFVFGVQVFSVPETDVDVGQIVRTSGYAA
                     APGLFAIFGIIPLLYVPALALALVWMLAAMVVSVRQAFDYSSTWRALGVCAAGWIVLA
                     AYIGGVVLLLRC"
     gene            16220..17485
                     /locus_tag="PLT86_04815"
     CDS             16220..17485
                     /locus_tag="PLT86_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT35966.1"
                     /translation="MRVLASLSALAFSAVAFAVAGLFVDRQLQALLDDRLAEALLAQA
                     KTVAGAVFLENTTDSVALALATGTLSATREQTGAELVVLLDARGRVLFTDPPVLGEHP
                     YIDLDNAALASAITGKPACGERFRIGNVDLKTAFAPVFNPFGEVVAIVGIEAPADFFG
                     ALRDVRRALVVTLGLGMALVAGLVAVVWHFWARSEASERALWDSQQLAMIGQMTATMA
                     HEVRNPLGIIKAAAERIRRKYGDGTELFDFIPDEVDRLDRLTEWYLRFARPGDLRLAK
                     ARLGPIVRDSISRVRSELEAAAIEVVVEDLAQSECLVDYDRLVQVNINILLNARQAIG
                     SNGRVEAKVIEKSGVVRVEVKDNGPGIPACIQPNVFQPFFTTKTAGSGLGLPVMKQVV
                     EALGGRVGLNSRPGEGSTVWYELPCAKNT"
     gene            17508..17846
                     /locus_tag="PLT86_04820"
     CDS             17508..17846
                     /locus_tag="PLT86_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35967.1"
                     /translation="MQRDKVGKIVPIGKGLCRVIVRSRKLAERLSSVPGVEVRAQDAE
                     YLGWWIIFPESMRALIEPVFREKTIVTEHASQLSLLDMDADDGGNSQEESPQKCGDGA
                     GEGNGSGTPG"
     gene            17824..>19087
                     /locus_tag="PLT86_04825"
     CDS             17824..>19087
                     /locus_tag="PLT86_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT35968.1"
                     /translation="MARVLLVDDERKLAVLLAGELTDAGHSVLTAQDGRTAIQLVKEQ
                     AFDVVVTDIRMEEVDGLALLRAVKDTCPDTDVIMMTAYASIETTVEALRKGAADYLIK
                     PFPPEELILAVERVVERQRLALENRQLKRELASAESEFIAASDSMKAVADVVSRIAKT
                     DTSVLITGASGTGKEVVARAIHARSKRKDGPFVAVNCAALPEPLLESELFGHERGAFT
                     GADRRKLGRFELADKGTLFLDEIGEIGQSIQVKLLRALETKTFERVGGTTNVSVDVRI
                     IAATNKDLPTAIANKQFREDLYYRLNVFPITLPPLKERVADIRALAAHFVAPTGLKLS
                     DAALRRLEEYEWPGNVRELRNVLERAVILCDGDTILPEHLSLNPISRQPGNDGGAADL
                     NLERRERKALEEALARAKGNKAEAARLLG"
BASE COUNT         3992 a         5391 c         5711 g         3993 t
ORIGIN      
        1 gggtacgtcc gtgaacgcgg cagccgggaa agcatccctt acgtcagcgt ctacatagag
       61 ggtttgcgtc tgggcggaat gacaaacgcg gcaggttact acgtcatcaa tggcgtcccg
      121 ccgggccgcc atgtgatcgt cgccgccatg gtcggttaca acgaatcgcg gcagacgttg
      181 gtcgtcgcca cggaaccgct cgtgcacaac attgagctgt ccacggaagc gattgaggtt
      241 gcggcagtgg aagtgcaggc ggaacgcgcc cgtgcccctt cgctcgaaat cattcccagc
      301 agagaaacgc ttcgcggtgt ggatttgaag attgctccgg cggcagtgga ggccgacccg
      361 attcggacac tgcatacgct cccgggggtg gtgcaactgt ccgatttcaa caccgggttg
      421 tacgtccgcg gaggaacgcc ggaccaaaac ctgatcctgg tggatggttc cgaactgtac
      481 aacgtgtcgc acatgttcgg tcttttctcc acatttcccg cggatgcgat caaaaccacc
      541 gaattgctca tggggggata cccggcacaa tacggtggcc ggctctcctc cgtgctcaac
      601 gtcatcacgg acgaaggaaa caaggagcga ttcaaaggct caggcggggt tagcctgctg
      661 agctcccgca tgacattgca gggcccgttc gcgaaaggct cgtttctcgt gtcgggacgg
      721 cggacgtacc tggagcccgt gttgtgggct gcgagccgct cgagcgatgc gttcgacggc
      781 atggggtatt tcttctacga cttccagggc aaggtacatc agattctttc gcactccgat
      841 cagttcgcca tcgccggata catgggcaaa gatgcgttca aatacacgaa cgatttcatg
      901 gacacccgtt tcgactgggg caacaaatcg gcctcgctga gctggaatca cctgttttcc
      961 ggtgaactct actcccattt gcaggcgaat cttagccggt tcgtttcgca cactacgttc
     1021 aagatcgagg atttcggcgc cacggatcat aacgagctct tcgacacgga tctcaggggt
     1081 gatcttacct ggttcgccaa cgagcgccat acgttcgaaa ccggcgttca tgtgaaacga
     1141 aacaccatgc ggttcgggca ggaatacgcg ggacaacgtt acgaagcatt caatcttatc
     1201 gcgtacgttc ccagcgcata tctgcaggat agctggagac cgtggccatt cctcacgctg
     1261 caacccggtc tgagggcgac gctcttccgg agccggcttc gagacacaac tgggtctgca
     1321 acctattcag cattcgaacc ccggttcacc gtacggtacc aattggggga acaaaccttt
     1381 ctgaaggcgg ccaccgggcg atacagccag tttattttcc gcgtgcccag agaatatgag
     1441 ggcatctcga tgatgtccga catctggttc acatgtgaca atacgccggg agggtatccc
     1501 cagcgcgcgt ggcactacgt gctggggctg gaaacgaagg tacggagcga tgtcgatttg
     1561 tcgcttgaag cgtattacaa ggattacgca gggctcgtgg agttcaatgc gtcacagacc
     1621 gccccgcaga gcgtcgaaga cgcactcctg aggggtgacg gatatgcata cggtctggat
     1681 gttaacgtaa agaaacgagc cggccgaaac acgggatggc tgtcgtactc gatgagttgg
     1741 accgttcgaa cgatcgacgg cctgaacctc gacgagaacg ggaacccgca accgttctac
     1801 ccgaaattcg acgcaagaca tcatctggac gcggtgtaca gctttgatct atcgcgccat
     1861 tggaccctca acagccgcat gtcattgtcg tcagggcagg ggtatacaaa agtactcggg
     1921 cggtatgtgg tggaggatcc tcttttcacc cagggtctct actaccgggg acagaaaaat
     1981 ggggcccgtt tgccctacta catgcgtctg gacgtaggtg cgaagggtag ttaccgattc
     2041 tggggagtca ggtggatgcc gttcttccag attgtgaacg ttctcaatcg cgcgaatgag
     2101 ttcaacagat actatgacag cggcgacgcg acgaagaacc caccggaaac cggccgcgaa
     2161 cgagcgatcc cccagttgcc gttccttcca accttcggcg tggatgtgga gttctgatgg
     2221 caaggagact ctggctttcg gttgcgttgt tcgcggttgt ggcgcaatca gggtgcattc
     2281 tcaacccggt caaacccgaa aaaaagaacg ttcccgagct cttcctgcaa gggtttctgg
     2341 tcgccggcgg cccgatcgac aacatcatcc tccgcaaaac aatcccgccg gagatgtatt
     2401 acgacttcat gccgctcgga aggtacgcag tgtcgggtgc cgaggtcgtg atcaccatcg
     2461 atggcgcccc ccacccgctc gcagaactcc atcccgggac gtacggtgac ccaaacctga
     2521 ttgctcgctc aggggctacg tgtgaaatcg aggtgcgttt ccccgcaaac cacgaattca
     2581 ccgggcggag gttgtacggc gccacgacga ttccagaaaa gccgcgtgcg tcggtgacaa
     2641 ttcacccgta ctacgtgcaa cggggagccc atctcaacgg caatgccgtc accgggctcg
     2701 tatttccgcg ggaactctcc cacccggatt ccttcggcac gactgcggaa aaggagccgt
     2761 tccggctttc gtggccgcta agttcttcgg caaccgggta catggtggtc tcaactgccg
     2821 tggatacgca gggtaccggg cttctgcggc aacgagatta cgaggagtat cttgacgggg
     2881 tgttccggga ccctgtgaag cgccagggca agcggacggc gggattctac gttcttcccg
     2941 attcctcaca gagcaccgtg tactggctgc tgttcagtta ccagggctgg catgatgtgt
     3001 cagtcgtggc ctgcgacacc gggtactggg actatatggt gacagtgctc aatgacgccg
     3061 gaaccggtgc tgattcggat gccggggtga agctgaacgt tcagggaggg ctcgggattt
     3121 tctgttcgta cgccgccgat acgacgcgtg tgtacatcca gcctgagtgg ctcccgtcaa
     3181 cccgctgacg cacgaaaaac aaaagggcgc ccgcaaggcg ccccgtgtga accccgatgg
     3241 tatgcccgct acgccgagat tgtgtcctgc ccgccgaggt agggcctgag cacttccgga
     3301 atcacgatgc tcccatcctc aagctggtag ttttccatta tcgcaatcat cacgcgcggt
     3361 aacgcgagtc ccgagccgtt cagcgtgtgg acgtattccg gcctggcgtt cggggccggc
     3421 cggtaccgga tattcgcacg acgcgcctga aagtcgcgga aattgctgca ggagctgacc
     3481 tccagccatt cgccgcaacc tgccgcccac acttccacat cgtatttcat tgccgcggtg
     3541 aaactcagat cgccggtgca catttgcaca atgcgatgcg gaagcgcgag ccctctgcac
     3601 acatcccggg catgttcgac aagagacgtc agctctgcat cggaggttgc cggctccacg
     3661 aacttcacca tctcgacctt gtcaaactcg tggccgcgct taataccgcg gacgtctttt
     3721 cccgcggaca ttttctcccg acggaagcag ggggtgtacg ccacgtgata gagcggtagc
     3781 tgggattttt ccagcacttc gccagcgtac aggttcgtga caggcacctc cgccgtggga
     3841 accagccaga agtcttcctc ggcgtcgtgg taaagattct caccgaattt tggaagctgg
     3901 ccggtgccta tcagacaatc gtgtcgtacc acggcgggag ggtagacttc cacgtacccg
     3961 tgcctcgtcc ggtggaggtc gagcatccat gtgatgagtg cgcgttggag gcgtgcgcca
     4021 tctcccacga gaacatagaa ccttgagcca cttattttca cgccgcgctc aaaatcgaga
     4081 atcccgaggt ccggcccgat ttcccaatgg ggacggggtt cgaaggtgaa cgtgggaaga
     4141 tcaccctcca tggcagtcac gacgttctct gattcgtctc tccccaccgg gacgtccgga
     4201 tgaggaaggt tgggcacctc cagcaggcgg gcgttgagtc gctcttccac tgcgcgcacc
     4261 tgctcatcga gaacagcaat gcgatcgccg acttcacgca tctcgagaat ccgtgactgc
     4321 cgttccgctg agtccttgac ggcgctgatc gcctttgaag cagcattgcg gcgcgccttg
     4381 agttcctcga cctcgcgaat caggttcctt cgcgcttcgt caagggagag gatttcgtct
     4441 atcggcgcgt cagtgaaaag cttcgcgatt tcccgtttta cgaaatccgt ctgttctctg
     4501 atcaatctca gatcaatcat ggcaacgtct ctccgcaatg cggtgcgaaa tccaccggca
     4561 gcacaatcag tcgtcttccg ccgacggatg ttgaagctgg tttgcgcggt ccgccatccg
     4621 taacgcttct atgagttcgt caaggttccc atcaagtatc tcgctcaggt tgtgcactgt
     4681 caacttgatg cggtggtcgg tgacgcggcc ctgcgggaag ttgtaggtcc gtattttttc
     4741 gctccgctca cccgtaccaa cctgcgccct ccgcgcggcg gcctcttcgg attgctgctg
     4801 gatgatcgca cggtcaagaa gacgcgcgcg gagcactttc atagccttcg ctttgttttt
     4861 caactgcgaa cgctcgtcct ggcattggac gacaagcccc gtgggcaaat gggtaatcct
     4921 gatggccgaa tctgcggtgt tcacgctctg ccccccgggg ccgctggagc ggaacacgtc
     4981 tatcctcaga tctgccgggt cgatctccac ttccacgtcg tcggcttcgg gcaatacggc
     5041 gacagaagca gtgctggtgt gtattcttcc ctgggcctcg gttgcaggca cgcgttgaac
     5101 cctgtggacg ccgctttcgt acttcaggcg gccgtacgcg ccttctcccg agatctgggc
     5161 gatgacttct ttcagtccgc ccgcagaggc ttcgctcgtg ctcatgactt ccagcgacca
     5221 tcctttggat tccgcatacc gggaatacat gcgaaaaagg tcggccgcaa acaacgctgc
     5281 ctcgtcgcca ccggtaccgc cgcggatctc aaggatcgta tttctgtcgt cgttgggatc
     5341 cttgggaatc agttgttcct tcagcgtctc ttcgagactt gccgcgcggt cttccagaac
     5401 gacgatctcc tccttcgcca tcgcgatcag gtccctgtcg ttttcctctt ccacaattcg
     5461 ccgggcttcc gcaagctggg cgagtacgtc cgcgtaccgt cggcccgttt ccacaatcgg
     5521 tgcaagctgc cggtgttctt tcgcgagttt ttggtactgg gcgatatccg tggtcgacgc
     5581 cggatcggag agaagccgct cgatctcggt gaatcgagtc tcaatggcgg cgaggcgatc
     5641 caacatttcc gtgttccttc ctctatatca ttcagacggc gcaacttacc gggccaccgg
     5701 tttcctgtgg aagaccgacg gctgcgcgca cagcggcgat agcgacggaa agctgatcat
     5761 cgttcggttc gcgtgtcgtg aggcgctgca acaagagtcc cggtttcgaa atgtaacgcg
     5821 cccggcgaga attccgggat tttccggttg ccttcaaaac ctcatagcca agacccgcaa
     5881 cgaccgggag gcaggcgagg tgaaggagaa agcgcgtctc gaggtccggc gcgttacccg
     5941 cataaagcgc gtataacgag tcagtgatcg cgtagaacgc gatcgccacc gccacgacga
     6001 ggaaaacgaa actggttccg caacggggat ggaaacgtgg aaaccctctc gccgtctgcg
     6061 ccgtgagttc cgagtccaat cctgattcga acgctgccac taccttgtgt tccgcgccgt
     6121 ggtactcaaa cacgcgcttc acgtccgcga tttttccaat cgcccagagg tagaaaagaa
     6181 aaacgagaat gcgcacggtg ccggcgaaga ggttgtacgc caccggacgg ttcgtcagac
     6241 cggcgagagt ggccaacgcc ataggaagga acacgaaaag gccaaccccg agcccgaacg
     6301 atatcaaaaa aagaagcgcc tccgccgggg atatccgatc cttcaccgga gtatcgcatc
     6361 catcggaggc tgcaacgttc gcgctgtacg tcagagcatt caggccgatg ccaagcatct
     6421 caaaaaaaga aactacgcca cgcagaaccg gcagcgacaa ccagcgtgtg cgcgccacca
     6481 gattcacgta gtcccgggtt tcaacagcga ttccgcgagg cgtgcgcaag gcgatggcaa
     6541 tggaccgggg agaacgtatc atgacccctt cgatcagcgc ttgcccgccg accgaaagat
     6601 cgagtctggt tctgtctgta atcgagtccg tgcgatctgc gggaggagcg gattccattc
     6661 tgcccggcgg ttacttcgct tccgaacggt tgtaccgacg tcggaaggca tctactcttc
     6721 cagcagtgtc tatcagtttc tgcttgccgg tgaagaaggg atggcacgca ccacagattt
     6781 cgacgcgaag gttcttcttc gttgaggacg tctgaacgac gttaccacac gcgcaggcga
     6841 tggtcgtggc gtgatattca ggatggattc cggatttcat caggtgtgct ccacacgttc
     6901 taagggtccg cacgtttgtc tgaaaaagca aactggattt gaaaatataa ctttcgatgc
     6961 acctaacggc caaattctgg cgattgaccc gggcacccgg tcgcgtcgat cagccgttca
     7021 tcatgtcgag gaattcgtga ttcgtctttg tgccgtgcat ccggtcgata aggaattcca
     7081 ttgcctccgc ggaactcatc tgcgccagga acttccgaag tatccacaca cgatgcagtt
     7141 cgaattcggt gaggagaagc tcttcgcgac gcgtccctga tttgttcaca tcaatcgcgg
     7201 ggaagatgcg ccgatccgaa agcttacggt cgagcacgag ttccatgttg cccgttccct
     7261 tgaattcctc gaaaatcacc tcgtccatac ggctgcccgt atcaatgagc gcggtggcga
     7321 tgatcgtaag cgaaccgccc tcttcgatgt ttcgcgcagc tccgaaaaac cgcttcgggc
     7381 catgcaatgc gttcgcatcc acaccgccgg agagaatttt ccccgagtga ggtaccaccg
     7441 tgttgtgggc gcgggcaagt ctggtgatgc tgtccagcaa aatcaccaca tcgtgcccgt
     7501 attcaacaag ccgccgtgcc ttctcgataa ccatgtccga aacctgcacg tggcggttcg
     7561 gcggctcgtc gaacgtggag ctgatcacct cgccatccac cgaccgctgc atgtcagtca
     7621 cttcttccgg ccgttcatcg ataagcagaa cgatcagttt gatatcaggg tgattgtgcg
     7681 tgatcgcgtt tgcggttttc tgcagaagca tcgtcttgcc ggctttcggc ggcgacacga
     7741 tgaggccccg ctgacccata ccgatcggcg caagaaggtt tacgatgcgc gtcgaaagct
     7801 cgttcgggta atgctccaga ttgatgcggc ggttcgggaa cagcggcgta aggttatcaa
     7861 aaagcgtctt ctctttggcg agatcggggt tctcaaaatt gaccgcctcc acacgaagca
     7921 atgcgaaatg ccgttcgttt tctttcggtg gacggatttg gccgctgacc gtatcgcccg
     7981 ttcgaagacc aaaacgtttg atctgagact ggctgacgta aatatcgtcc gggcccggca
     8041 ggtagttgta gtcaggcgac cgaaggaagc cgttcccttg ctccagaatc tcgagcacac
     8101 cctcggcgaa tatgaagcca cttttcgcgg tttccgcctc caagatcttg aaaataagtt
     8161 cctgtttgcg gtagctggtg tactcgggta tgccgagggc gtgcgccagt tcatggagct
     8221 cggcgatggt cttcttcttc aattcgatga tgttcaaggg tgtgcctccg gcaggagatt
     8281 tggtggaacc tcgggctttg cgtgtaggtc gcgggcccat aaaggggcat tccatataag
     8341 ctcgttccgg atacggatac gcgacgtacc gggacgagtt ctgttagtgc gttcgggaag
     8401 ccagagaaac gattatcgaa tggaagtcag gtcttttagt cgttttcgcc agttattcgt
     8461 gctcgcgaaa gagagcgcga cgcgcattga gcgaacgaaa caaaacctgc cggcacaatc
     8521 tgagttgcgc cgctaccata tgtccttcca gatggtatct caagggcatt cgtcaagagg
     8581 tcacttgaca ggcgaacagc aagaaagttc acggaaaatt gcggggtagt tgcgtgtctc
     8641 taacataggg ccttgagccg gaagtgtcaa gtccgctcat tcccgatcac gcgaagaaca
     8701 gactcgcgga ccttctcact ttctgcggcg tcgatcagtt tctccccgct gctcgttact
     8761 acatagaacg cgtctgccac gcgatccccg tctgttccaa tccgggccat gttgatatcg
     8821 agcccgttct ccgaaatcgc tctgctgatg tcgtaaagca gcccgatcct gtctattgca
     8881 aacacatcaa tcacactatt ggtttcactt accgtgttgt caattaccac gtgagagggt
     8941 gccatgccgg ctttgggctt gatccgagcc catcttcttc gatgccgcgc tatgagttct
     9001 gcaaccacgg cctgccctgt ccacacagac cggaacgtgt cgcgaagagt actcacgaac
     9061 gcctcgtccc ttatgtcgct ctctcccacc acctctactc cgaatacgtc aacgatgatt
     9121 ccgtccgtgc gcgtatacgc atcagcgtag aggatattca ggttgttgac ggcgagtact
     9181 ccgcagatgt cgctgaggag gtacctgcgg tctctcgtat acacgatcac gcgtgcgtgg
     9241 ttacctgcag gcgctacgtc gaccagcaca ggttcatttt tcagggaatc cgctcgcagc
     9301 gcgtgagtag ctgcttcgtc gagggagtgc atcagcgaat agcgtcgcgg catacccgaa
     9361 agatggcgtt ccgccgcttc ttcgccgatt ctttcggcaa gaaggttgcg aaacttctga
     9421 gggctctcca gtgtgcggag ggcagcgaac tcaggcccga gcccgcgcgt ttccacaacc
     9481 tcgcgaagac gctgatgaag cgtatccaga agctgacatt tccatcgcga aagcggtcgc
     9541 gcctgcgttg cgttcatatc cgcccacgta agcacgtaca gcatgtcaag cgtgtcggtg
     9601 tcgctgaaac gttccgcctc agccctgatc atcgcgagat catcgatgtc acggcgttgc
     9661 gacaggtggc ttaaccgaag atggtgtgcc acgagaaagg caacgagatc ctggtcggct
     9721 ttttccagac cgagtcttcg agtgatggta atcgacatct ctgcgccgcg ctccgcgtgc
     9781 ccgtgcccgc cggacttgcc tacgtcgtgc agaagcgcgg caagatggag gagatgcttc
     9841 ctcgagagag aacggtacac tgttgccact cgcgaatcgt cgtcggtcaa cgcgagctcg
     9901 ctcaagcggt caatcacgat gatggagtgc tcgtccgccg tgtaggcgtg gtagtagtca
     9961 tgttgcacct gacaggtcaa gcgcccgaac tcaggaatga acgcacccag caaacctgtc
    10021 cagtgcatac ggcggagtac cgcccccaga tgcgccggtt cacccaggat tgacagaaag
    10081 acagccgcat tttccgggtc cgttcggaag tcctcatcga ccagtgcgag ttcgtcgtaa
    10141 atcgcccacg acgccgtctc gctcatgatg gcgccaaagc gctgcatatc ggcgaacagg
    10201 ctcaacatcc ggcgcggatt ggtggcgaaa aagcctttcc tgcgcttggg gaggaggatc
    10261 tttccgcgaa cgtagaccgc cccgtccggg agtacacgcc gccggacaat atcggtcaca
    10321 gtctggatcc cgcgtcgccg gcggagtgcc tgatggcaca cggcatccga tatccgtacg
    10381 atctcccgcg ccgcgagata gtaatcccgc atgaaatgct ccactcccag tgcggcagcg
    10441 tcgtcccggt atccgaatgc tgcagccacc ttgggctgag ctgtgaagtc gagccggtcg
    10501 tgcttcctct tgttcacaaa gtggagctcg ttgcgcacct tccagagcag atcaatcgac
    10561 tgctccagaa gacggaaatc tcgtttcgat atgagatgat gctcaacaag cgcttccagc
    10621 gaagcggttc cgtgccttgc acttgcaatc cactgcagag cgtgaacgtc ccggagtccg
    10681 ccggtagatt ccttgacatc tggttccagc acctgggcgt acgaaccgtg cgcctcacgc
    10741 cgcgcatgga gctggcgaac gactttctcc gaaaactccc acccgccgtc cgccatgaaa
    10801 cgcgcgcgga aactgttcac aaaccgaccg aacaggggct cgagcccggc gagaaaacgc
    10861 gcgtccacga cggcggtctg cgcgatgtca tccctggaag caaattcgag agcgctttgc
    10921 agacttcggg cggaatggcc cagatcaaac ccgacgtccc agagcaggtg cagaaccccg
    10981 tgcacaatcg gagactgctc cggtacctcg tcggcgaaca ggaacaacag gtcaacatcc
    11041 gagcagatgt tgagggcgcc tctcccgtac cctccgagag caatcaacgc acactcgccc
    11101 ggagttcctt ccccgttgag catcaccgca cggtatgcct cgataacgac tgcgtccacc
    11161 acggcggtcc attgttgcac cacatgatgt cccgaagcgc ctgcccggtg ctcgtagtgc
    11221 aaccgctcac gttcccgctc gacgaactcc cggaaaatcc gcaactgaat cgaggtgctt
    11281 ttctcttccg aataaccttt tgcgcgcgct tcccgggaaa gcagttcgag gctctgacgc
    11341 aaatcggtca tcatcggcgg gacgcccgtt aaggagtttt gttccgagtg gggtgattgt
    11401 gtaacatgaa cgttacaatg ctgttactga gccggtgaat gcaacgtcgg cgttgccctc
    11461 gaggaacacg tcttccgcgt gttctcctcg attcacgaag tgaatggtca aaggcaaacc
    11521 gctttgagtg ataccgacaa ccggagaatc gaaaagcccg aggaacgatc cgatgatagc
    11581 cgcggcagtt gtgccggttc cgcaggcaag cgtttcgtct tccactcccc tttcgtacgt
    11641 tctgatgcgg aacgtgtgcc tgtccagagg cgtgatgaag ttggcgtttg tgcccgcagg
    11701 gcggaacact tcgtggtacc gtatctcgcg cccgatgccg actacatcca cggcgtcaac
    11761 atcagccacg acctccaccg cgtgagggac tccggtgtta agcgtgtgca gtgttcgcgt
    11821 tcctccggag agctcgacgt ggttgttcag cgaaagcgaa tgcggcgggg tcatctggag
    11881 cttcacgttc gcaccccgga tctccgcctc gtgggttcct gccgctgata cgaaacgcat
    11941 ccgggcaggg gcaattccgt ggaggtacgc aaagcgggcg atacaacgac cgccattgcc
    12001 gcacatttcc gcttccccgc catcagcgtt ataatatcgc atccggaaat gcgcgttctc
    12061 ggcattttcc agaagcagga caccgtcggc tccaacggca agcctgcgct ggcacacgtc
    12121 gcgaacgaat cgcgctctcg gttcaccctc aggcagggag gtgttccggt tgtcgattac
    12181 gacaaaatcg ttacccgctc cgaccatttt tgtaaacagg atatccaatg cggtgtcctc
    12241 gaatacgttg cggcaggtgg cagattggcg gtcgtgagag aacggctttc cgggacatgg
    12301 aaagtgcttg agactatcaa aaaatcgccc tcggagacct ttgacgtcaa ggggacatcg
    12361 ggcgtccgcc gcttgtgcaa aacgcggcaa atgttgcatt ttcaacactg cgggagtcag
    12421 cgctcatctg ggccttgaaa agggattccg ggcccgcatg tgcaggcttt tgtaatcttg
    12481 gcaggagata atcgagatgg cggcgtcgaa gagaataatc gccattgatc tgggcgcatc
    12541 aagcggacgc acgattctgg gtgaactggg aaagaaactc agcctgaggg agattcaccg
    12601 gtttccgaac gggcctgttt ccgataacgg ccgcattcgc tgggatttcg accgtctctg
    12661 gcaatccgtg caggagggca tccggcgggc ttccgtggat gccggcggag acatcgcaag
    12721 ccttggcctg gatacgtggg gcgtggacta cggtcttttg ggcgcggacg ggcgattgct
    12781 ggaaccgccg gtccactatc gggattcgcg cacgaacggg atgatggaat tcgcgttcaa
    12841 aatggtccct cgcaaggaga tatacgagac gacgggtctg cagttcctcc aactgaatac
    12901 gctttatcaa ctgctcgcgg aacagatgtc tggctcgggg cttctcgaga aggcgaaaac
    12961 gctgctgatg atccccgacg tttttgatta cctcctgacg ggggtcgcaa aggcggaatt
    13021 cacggacgtc actactactc agtgttacga ccctcgagcc cgcggctggg cccgctccat
    13081 gctggaacgc cttggaatac cgacgcactt cttgccggag atcgtttccc cgggtacacc
    13141 gctgggctct gtccgtccgg agctggctgc ccggcttggg ttgcagggcg ccagagtcat
    13201 cgctcccgga tgtcacgata cggcgtccgc ggtggcggca gttcccgctc gtgacggcgg
    13261 gaagtgggcg tatctgagct cgggaacgtg gtcgctgatg ggcgtggaac tgccagagcc
    13321 gcacattacg gagaccgcgc ttgcgtacaa cttcacgaac gaaggcggta tcgggggcac
    13381 gttccggttc ctgaaaaaca tcagcggcct ctggttgctg caggaatctc tccggtactg
    13441 gcgggaacat gatggtgagg atatctcata tgccgaagca gtcgcgcggg cgaaagcggc
    13501 gcgtccattc cagtgctggg ttgatcctga tgcgccggac tttaacacgc cggcctcgat
    13561 gccagaggct atttctcgat attgcgcaaa caccgggcag gtggtacccc ggaccaggga
    13621 cgaggttctg cgtaccgtat ttgagagcct cgccctcaaa taccggtcgg tgtttgacat
    13681 gctgagggaa atggtcggcg acattcaggt gcttcacatt gtcggtggag gatcggcgaa
    13741 cgaactgttg aaccagttca ccgccaacgc cctcggtgtt caggtggtgg ccgggcccgt
    13801 ggaagcaacg gcgattggca atctgatggt gcaggcgatg gcgttgggag aagtacgctc
    13861 aacggcggaa atcagaacgg tcgtccggga ttcattcgca gttgcgctgt tcgagccaca
    13921 tgattctgcc gactgggaag acgcgtacgg acgctggcgg gaggtaatcg cgaaatccgt
    13981 cgcgtagtac gggaggcacc gacatcgtcg caaacaccca cgattttcaa agcgccctgc
    14041 aaaactcgta cactgaatct gcggggtgcc ggtgaccgga atccgaaaag ccgcaataac
    14101 tggcagaaca aaaggggggg cagggaaacg gacacgctct ggcaggcgga catggaagca
    14161 accgaacgcg tcaaaggcgc aaacgggacc gtactcatag tcgatgatga tccggcgatt
    14221 cgcaagttgc tggcaatcgc gctggaaaga gcgggtttcc aggtgcggca agcggaggga
    14281 taccgccagt gctacggcga gatgagtgac acggcgagcg ttgacgcagt cctcctcgat
    14341 atcatgttgc ccgaagtaaa cgggctggaa atcctgcgcc attgttccgg actggattcc
    14401 ccgcccgcta tcgtaatgat gaccgcctca acggatgtct cagacgccgt cagcgccctg
    14461 aaactcggtg cgttcgatta cctgatcaag ccggatgaca cgagcaatct gcccaagctt
    14521 accggagtac tgaggaacgc agtctccttc acacgttcaa aacgcgagat cgttcggtta
    14581 aagcgggaac tggtagcgcg cggccgcccg gcggaaattg ttggcacctc ggcacccatg
    14641 caaagggtct tcgaggcgat ccgccaggtc gctgacagcg atgcctctgt tctgatcaca
    14701 ggggaaagcg gtaccgggaa ggagttggtg gcaaaggcga ttcacgaggc aagcgctcgt
    14761 tccaggggac cttttgtcgt cgtgaattgc tcggcgattc caatcacgct cattgaaagc
    14821 gagctgttcg ggcatgaaaa aggagcattc acaggcgcaa tagctcaaaa gaggggcaaa
    14881 ttcgagatcg cgcacggcgg gaccattttc ctcgacgaga taggagacat ggattccggt
    14941 tcgcagacaa ggttgctgcg tgtgctccag gaacaggcgt tcgagcgcgt aggaggcacg
    15001 cacaccatca atgttgacgt tcgggtcatt gccgcaacca atcaggacct gcaggcccgt
    15061 gttcgagaat cggcattccg gttggacctg ttctaccgga tcgcggtctt tgccatctca
    15121 ttgcccccgc tacgcgatcg aacggaggat attccgctcc ttgccgcaca tttcctgaac
    15181 cttcacgcgt cgaaagccca caaagaagct accgggttcg aaccggcggc gatgaacgct
    15241 cttgtccggc acgcatggcc ggggaatgtt cgggaactgg gaaatgtgat ccaacatgcg
    15301 gtaatcgttt cagaaggacc aaccatccgg cttgaacatc tcccgccaca cctggtgcag
    15361 gcgagtgttc ccgaaacgcc tgcgggagag aacgccgtgc ttcttcggga tccttccaca
    15421 cacgatattc gtccgctgaa agaagtggag aaggaggtga tgaaacgggc gcttgaggcc
    15481 acggagggca actttgctct ggctgccatg aagcttcaaa tcggccgggc cacgttgtac
    15541 cgcaaggtga aggaatacgg gttggacgca gacggaactt gacggttctg agcgtgcatg
    15601 ttccccatct cgactccgtg ggtgtgcaaa aggcgatgat ggagggcagt atgtctcctt
    15661 atgtgaatcg aatacggcgc gcactgaagc tcgatcccgc attgttcgag gaagtggaag
    15721 cggacaggca cgcaacgggc gaagccgttg gtgtagtcct gttatcgagc ggcgcgacgg
    15781 gccttggagc gattcctcag acaagcatcg gatcgctcgc gattctgttt ctcgcggtgc
    15841 tcgtcgggtg gtgtgcatgg gtatatgtga cattcgtgtt cggggttcag gtgttttctg
    15901 tgcccgaaac ggatgttgac gtgggccaga tagtacgcac gtcggggtac gccgctgcgc
    15961 ccggtctctt cgcgattttc ggtatcattc cccttctcta tgttcccgcg cttgcgctgg
    16021 cgctggtgtg gatgctcgcc gcaatggttg tgtcggttcg ccaggcgttc gactactcaa
    16081 gtacctggcg tgctctcggc gtgtgcgcag ccggttggat agttcttgcg gcgtacatcg
    16141 gcggggttgt tctcctgttg cggtgctgat aagcgcgggc acttaatcga agaggccacg
    16201 aagcatggaa gcgtcccgtg tgcgggtttt ggcttccctt tccgcgctcg cgttttccgc
    16261 tgttgcgttt gcggtagccg ggctttttgt tgaccggcag cttcaggccc ttttggacga
    16321 tcgtctggcc gaggcgttgc tggcacaggc gaaaaccgtc gcgggcgcgg tttttctgga
    16381 aaacacaaca gactcggttg cactggcgct ggcaacgggt accctgtcgg cgacgcggga
    16441 acagaccggt gctgagttgg tggtgcttct ggacgcgcgt ggcagggttt tgttcactga
    16501 ccctccggtt ctcggagagc atccgtacat cgatctggat aacgccgcgc tcgcgtccgc
    16561 catcaccgga aaacccgctt gcggagagcg tttccgcatc gggaatgtgg atctgaaaac
    16621 tgccttcgcg ccggtgttca acccgttcgg cgaagtagtg gcgatcgtgg gcatcgaagc
    16681 gccggcggat ttcttcggag cacttcgtga cgttcggcgg gcgcttgttg tcacactggg
    16741 gctgggcatg gcgctcgtcg cagggttggt cgctgtggtg tggcatttct gggcgcgaag
    16801 tgaggcttct gaacgcgcgc tgtgggatag tcagcagctg gcgatgatcg ggcagatgac
    16861 cgcgacgatg gcgcatgagg tgcggaaccc gctcgggatc ataaaagcgg cggcagaacg
    16921 gattcgtcgt aagtacggcg acggaacaga gcttttcgat ttcattcctg atgaagtgga
    16981 tcgactggat cgtcttaccg aatggtatct gcgattcgcc aggccgggtg atctgaggct
    17041 tgcgaaagcc agactgggtc caatcgtccg ggactcgatt tcccgtgtgc gcagtgaact
    17101 ggaagcggca gcgattgaag tggtggtgga ggatctggcg caatcagaat gcctggtgga
    17161 ctatgatcgc cttgtgcagg tgaacatcaa catccttctg aacgcccgtc aggcgattgg
    17221 ctcaaacgga agggtcgagg caaaagtcat cgaaaaatcg ggcgtggtga gggtggaagt
    17281 gaaagacaac gggcccggga ttccggcctg tattcagccc aacgtgttcc agccgttctt
    17341 tacaacgaaa accgcgggca gcggactggg acttcccgtt atgaaacagg tcgttgaggc
    17401 ccttggcggc agagtggggc tgaacagcag gccgggagag ggaagcacgg tgtggtacga
    17461 actcccgtgt gcgaaaaaca cctgatcgaa ctgaaaaagg acgatagatg cagcgcgaca
    17521 aagttggcaa aatcgttccc ataggaaaag gtctgtgtcg cgtgatcgtt cgctcacgga
    17581 aactggcgga aaggctttcc agtgtgcccg gtgtggaggt cagagcgcaa gacgcggaat
    17641 acctggggtg gtggataata ttcccggagt caatgcgcgc cttgatcgaa ccagtattcc
    17701 gtgagaaaac gatcgtgacc gaacacgcct cgcagttgtc gctgctggac atggatgctg
    17761 atgacggtgg caattctcag gaggaatcgc ctcagaaatg cggcgacggc gcaggagaag
    17821 gaaatggctc gggtactcct ggttgacgac gaacggaagc ttgcggtact gcttgcgggc
    17881 gaactgaccg acgcggggca ctcggtgctt accgcgcagg acggtagaac tgccattcaa
    17941 ctggtgaagg aacaggcttt tgacgttgtc gtaacggata tccgtatgga ggaggttgac
    18001 ggtctcgcgc tgctcagggc ggtgaaagac acatgtccgg acaccgacgt aatcatgatg
    18061 acggcgtacg cgagtatcga gacgacagtc gaagccctga gaaagggagc ggcggactac
    18121 ctgatcaagc cctttccgcc cgaagaactt atcctggcag ttgagcgcgt cgtggaacga
    18181 cagaggcttg ccctcgagaa cagacaactc aaacgggaac tcgcctccgc agaatccgaa
    18241 ttcatcgcgg cgagcgattc catgaaagcc gtcgccgacg tcgtgtcccg gatcgcgaaa
    18301 acggatacga gcgtgctgat caccggcgca agcggaacgg gtaaggaagt cgttgcaagg
    18361 gccattcacg cacggagcaa gaggaaggac gggcccttcg tcgcggtgaa ctgcgcggcg
    18421 ttgccggagc cgctgctcga gagtgagctt tttggacacg aacgcggagc cttcaccggg
    18481 gccgaccgac gcaaactggg aaggtttgag ctggctgaca aaggcacgct gttcctcgat
    18541 gagattggag aaatcggtca aagcattcag gtcaaacttc tgcgcgcgct ggagacgaag
    18601 acattcgagc gcgtaggagg gaccacgaac gtcagcgtgg atgtgcggat catcgccgcc
    18661 acgaacaaag atcttcctac ggccatcgcg aacaagcagt tccgagagga cctgtactat
    18721 cgcctgaacg tgttcccgat aacgctgccg ccgttgaagg aacgggttgc ggatatccgc
    18781 gcgttagcag cacattttgt ggcgccgacc gggttgaaac tgagtgacgc cgccctgagg
    18841 cggcttgagg agtacgagtg gccgggaaac gtgcgtgagc tccgcaatgt tctggaacgg
    18901 gcggtcatac tttgcgacgg cgatacgatt ctgccggaac acttgtcgct gaatcccatc
    18961 tctcgccagc cggggaacga tggcggcgcg gcagacctga acctggaaag aagggaaagg
    19021 aaagccctgg aagaggcatt ggcccgcgcg aaaggaaaca aggcagaggc tgcgcggttg
    19081 ctcggaa
//
LOCUS       DAOSOO010000042        19085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2887_length_19085_cov_4.960168, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000042 DAOSOO010000000
VERSION     DAOSOO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_19085_cov_4.960168"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLT86_04830"
     CDS             <1..604
                     /locus_tag="PLT86_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35969.1"
                     /translation="GGVLLLTVARYYTPTGRLIQRDYGAGTAQYIFSGIAEGVRVDTS
                     QGPRFVTPLGRVVYGKGGISPDYAIPRNEPDPVLAKLYSSALFFRFVNDAANTVVVTP
                     QRGEEFRASQLVDDRVLGEFFDFCERMDSSFTKLKLEAEENSIRPHLAAAIAEKTWGQ
                     QGRYRVLAPFDSELSAARAHLVDARSLLNRSRSGRAHIGR"
     gene            complement(615..830)
                     /locus_tag="PLT86_04835"
     CDS             complement(615..830)
                     /locus_tag="PLT86_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35970.1"
                     /translation="MVPVTEQSALRVEGVFFFPHDAKTMGAAQLSVYVDAIRGNSVAC
                     VPVFVLGQKAFTRLLAYLLLFLRRKRV"
     gene            1047..1283
                     /locus_tag="PLT86_04840"
     CDS             1047..1283
                     /locus_tag="PLT86_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35971.1"
                     /translation="MSNPQGAAVFLDGVSANATTDAILPVRKAGPHVISVARPGFRAV
                     PSSVIVNLTEGSPQGLATFTLYPVRSDTTNGMAE"
     gene            1286..2452
                     /gene="galK"
                     /locus_tag="PLT86_04845"
     CDS             1286..2452
                     /gene="galK"
                     /locus_tag="PLT86_04845"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT35972.1"
                     /translation="MSGPTSLAEEACALFRATWQRGPECLASSPGRVNLIGEHTDYND
                     GFVFPVAINRRTFVAMGASRDGRSGFVSTSHPIPVLADCPPWRRMENDCWANYVAGVA
                     AQFANRNERVPAFRASVASDLPAGAGLSSSAALEAATAAALSQLVGASFSITELAALC
                     QKAEHEFAGVRCGIMDQMSSVAGGAIVLDCRSLAFQRVQIPTDLSLVILDSGIPRGLS
                     ATRYNERRGQCEEGVRVLKGRFSGIKALRDVTPGMLDACRKDLSETVFRRCRHVVTEN
                     VRVSAAAKAFAENDFPAVGKLLQESHVSMRDDYEISLPEIDLLVEIASSTPGCYGARL
                     TGAGFGGAVISLVDTGSADVFAEEVVEEYRKKTCKAGTAMRVVPDEGSRKEEPV"
     gene            2477..3454
                     /locus_tag="PLT86_04850"
     CDS             2477..3454
                     /locus_tag="PLT86_04850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOT35973.1"
                     /translation="MPTWNVFVTRRIPDAGLDLLRNAGVNFEVNPEDRVLTHEELLAG
                     VSGRDGVLCLLTDIVDESVLEAARGARVFANYAVGYNNVDVAAATKRGIMVTNTPGVL
                     TETTADMAWALIMATARRIVEGDRLVRAGEFHGWGPMMLLGMDVFGSTLGIVGAGRIG
                     CAVARRAQGFGMKVLYTDSAPNPEISSLNAIRVDLDELLKASDIVTLHVPLLEETRHL
                     IGKREFALMKNGAILVNTSRGPVVDERALVEALKSGSIAGAGLDVYEDEPQLAPGLAD
                     CKNAVLTPHTASATIATRNKMAVMAATNLLAALRGDTPPNLVNPEVLTG"
     gene            3492..4235
                     /locus_tag="PLT86_04855"
                     /pseudo
     CDS             3492..4235
                     /locus_tag="PLT86_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            4248..5297
                     /gene="purM"
                     /locus_tag="PLT86_04860"
     CDS             4248..5297
                     /gene="purM"
                     /locus_tag="PLT86_04860"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT35974.1"
                     /translation="MSPAEPLRTVTYADAGVDIEKANRTKELLKELVRRSYTPQVLTE
                     LGSFGGLFEADFGDVEDPVLVASTDGVGTKLKVAVTAGRHTTVGMDLVNHCVNDILVM
                     GARPLFFMDYIALGRHDTQIVVDVVEGLSTACTAVGCALLGGETAEMPDMYRPGEYDL
                     AGTIVGVVGKREVITGANVRHGDVLIGLMSDGLHTNGYSLARKLIFDIAGWNTDTFVP
                     EWGRTVADALLAPHRCYLHALLPLIARGMIHAMAHVTGGGITDNVPRILPEGLSARVR
                     TAAWDTPPIFRTLVRLGNLNAEEAFRAFNMGIGMVLVVDSTSADTVLGELTTAGETAW
                     LIGEVVSGDRNVQYV"
     gene            5290..5679
                     /locus_tag="PLT86_04865"
     CDS             5290..5679
                     /locus_tag="PLT86_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOT35975.1"
                     /translation="MSETPQSEPKSVLRRYRKSLLSVWALYSAALTVSMMVPGDSVPD
                     LGFFGWDKLAHLVVFIVLAFLTAGAFLTRDCVITIVMSWGVLLGCATEFAQALAPGRI
                     PSVGDAAANLIGTVFGALLVKRYLLRD"
     gene            complement(5680..6006)
                     /locus_tag="PLT86_04870"
     CDS             complement(5680..6006)
                     /locus_tag="PLT86_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOT35976.1"
                     /translation="MVAEETVSFITMIGPTEPNGGQNSVEVNLGEHVEGRYSNVVMIT
                     HTATEVILDFSVHVPGLPGPKVVSRIIITPEHAKRLLNALDDNIKKYEKRFGTIHHPE
                     ERNPPA"
     gene            6038..7519
                     /locus_tag="PLT86_04875"
     CDS             6038..7519
                     /locus_tag="PLT86_04875"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT35977.1"
                     /translation="MRSVAFEIGGKALLDGVSVTISPGEYVAVMGRNGSGKTTLIRHF
                     NGLLLPTSGECFAFGLPTRDPRCLREIRRRIGLVFQDPDDQMVAVTVEDEIAFGLENR
                     AVPPEEMDQRVEAVLDIFHLQHVRKRSTSALSGGEQQRVAIAAVWATNPDVLVLDEPT
                     SMLDLVSSVALLELLNEIRGSKAVVHVTQNLQEAARAERLIVMDGGRIVFDGPPQQAL
                     EDRDSLRGWGVVAAGRTASRNAPPGKSIAISTEALNHVRDDGNETNHVLKDVTFQALR
                     GSVLAIVGKSGSGKTTLAYHFNRLLEPTSGEVRIGERPARSIALHELRRIVGLTFQRV
                     DLQLFEASVLDDVAFGLVQRGMERAEAYRRARLFLHRMGLDPDSFGNRAPGTLSTGEK
                     RRVALAGVLVSEPEVIVLDEPTSGLDAASVEQLSEIVSELANQGKTLLIITHDLNFAA
                     RTADGVILVDEGRAEVLEGTDRLGELAATWLAETLSRFRSSAA"
     gene            7661..8320
                     /locus_tag="PLT86_04880"
     CDS             7661..8320
                     /locus_tag="PLT86_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOT35978.1"
                     /translation="MRARDNDKLTIVWYGLVRNASTVNPVDRMLRDGATVSPGEGIQL
                     FVSVKPAAFLYVIHQSPTGLLTVWWPSQSSGFNAYLEKERVLTLPARGKIHVLGAAQG
                     TEAVYLIAAFKPVTRVDKLTKEIQWLFASAQAFGSGVPARQVKQMLPRGLVWRLDANT
                     PVGLTDQRVPVATTVRCIGRIAGGGTATSTSDSQNTVIPEQVRGGDVVARVIRFQRQL
                     R"
     gene            complement(8323..9111)
                     /locus_tag="PLT86_04885"
     CDS             complement(8323..9111)
                     /locus_tag="PLT86_04885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT35979.1"
                     /translation="MSGFAFRRRFGRLGHVLRAHDAAKLPPEHLFAPLEEERGPEVGR
                     IEGLRKELLSSTATISDFHFGSGEAVASRVTRVAHECRYSSVPARWCRVLFRLVRKTK
                     PANCIELGTNLGISSAYLAAALKLNGSGRLFTIEGSPSLTECAKRNMTLLGYDNVQIV
                     NSSFETGLPDTLKEAGSVDFAFVDGHHDERATLDYAGMIIEASSEGAILVFDDIRWSS
                     GMARAWERIAADKSVTAVYDCYDIGAVVVREPGATRKEFTRIRA"
     gene            complement(9172..10983)
                     /gene="ftsH"
                     /locus_tag="PLT86_04890"
     CDS             complement(9172..10983)
                     /gene="ftsH"
                     /locus_tag="PLT86_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT35980.1"
                     /translation="MSGRLKFSLWYAVLAVMIVLAIRQYSKPPSKTLFYTEFIQAVRE
                     RKVTAVAIGEDEIEGRIVGAGDSVRSFNVVRIKDDKGLVSLLDEHGISYRGVIPGPWP
                     KILSWVLPMVVLIGFWIFFLRRMGGPSSGAMSIGKSKAKVYVETETKVTFADVAGIDE
                     AAEEVREVVEFLKEPARFQKLGGRIPKGVLLVGPPGTGKTLLARAVAGESGVPFFSLS
                     GSDFVEMFVGVGAARVRDLFQQAKGRSPCIVFIDELDALGKARGVNPLGGHDEREQTL
                     NQLLTEMDGFDDNTGVIIMAATNRPETLDQALLRPGRFDRQIVVDRPGIKGREEILRV
                     HARKVTLAPDVDLHQIAARTPGMVGADLANMINEAALLAARKNHPAVTMEDLNEAIDR
                     GIAGLERKSRVLNETERRITAFHEAGHALVAEALPHTDPVHRVSIIPRGVTSLGQTTY
                     QPTEDRYLMSKEELEDRLAVALAGRVAEEIVFGDVSTSAGTDFRRATRIAQHMVKDYG
                     MSRLGLVSYGEAPGFLAQPVGGGAVHYSEETAAEIDDEVRRIVSEGYQRVRELLENNT
                     DALHRLANALIERENLQGDEVREILRGSLKETETSAA"
     gene            11266..12618
                     /locus_tag="PLT86_04895"
     CDS             11266..12618
                     /locus_tag="PLT86_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOT35981.1"
                     /translation="MSEGLWASARSRLWCNDTRLAESTWGRVLLRLLRTSVIAGKGFW
                     NDECMLRATALAYTTLLSLVPLLAVAFSLFAAFPELENAYAEIRSFLNEYLAPGASKA
                     AISALNEFIANATTGTLAGIGTAALFVTVFLMLSSVEESLNRIWRAPKTRPILDRLIY
                     YLAIVILGPLFLAVSVNAAIQTVQRESLALNLVEPPFVSELWEYSLPLLASCALFTIL
                     FKAMPNTKVRWPAAVAGGIVSGVLFEIAKKAYTLYASNAIAYRTIYGAMSTVPLFLVW
                     IYVIWLVVLFGAEFAHGWQTIISYQRIATWGPTSQYHRELLAAALAVATTRRFEEGKP
                     GLTSQEMADIFDAPLGIVEEIVEILKSSSVLTESGRGILPAKDPDKLTLWDVVGPLRK
                     GAETRPIPEQLFREEVGPAVLFLEEVETGNEEPFRSITLAELAAKSAVAETTPDERGQ
                     "
     gene            12727..13116
                     /locus_tag="PLT86_04900"
     CDS             12727..13116
                     /locus_tag="PLT86_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35982.1"
                     /translation="MENLVSNLLSWVSSFFGNLYADAPIIWGSKYPAFLGELLLLIIV
                     IDLVIFGWRPLVKRFFPKSYGAVDYAFGQVTNLIWLCILVAFMIYIGGALGEAWGRFL
                     GVSTMIWSTVGVVALLLLGKLAVKRHR"
     gene            13237..13815
                     /locus_tag="PLT86_04905"
     CDS             13237..13815
                     /locus_tag="PLT86_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT35983.1"
                     /translation="MGVVATFFIALGLSADAFAVAVSNGFSSRRPRIGEAFRFGVAFG
                     SFQALMPLIGWLAGSSMAGLFEDTDHWVAFGLLAFIGGKMVYEGIWGEATNGEGKPVT
                     LWVLLILSVATSIDALAVGISLSLLSTPIFMPAMLIGVTTLCVSTAGFLLGKRLGRFF
                     GRRVEVAGGIILVAIGAKILIDHLTRGVGGQV"
     gene            13931..15736
                     /gene="lepA"
                     /locus_tag="PLT86_04910"
     CDS             13931..15736
                     /gene="lepA"
                     /locus_tag="PLT86_04910"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT35984.1"
                     /translation="MTDQKRIRNFSIIAHIDHGKSTLADRLLEFTGTLSSNEMQDQVL
                     DSMDLERERGITIKMHAIRMEYGAENGETYVLNLIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDAAQGVEAQTVSNLYQAISADLEIIPVINKIDLPSAQIPLVTQEIRDLLGVEEKD
                     ILLVSAKTGQGIRELLEAIVAHVPEPKGDPDAPLRALIFDSVYDSYRGAVVYVRVVDG
                     AIRPGQKVRLAATNAVHDVDEVGFLRLGRVARDSLQAGEVGFVVASIKTISEARVGDT
                     ILDNSAPAVVPLKGYQEVKPMLFAGIYPVDAEMYEDLRDSLARLKLNDASLVYEPESS
                     AALGFGFRCGFLGLLHMEIVQERLDREFGLNIITTVPNVRYRVVTTSDEVIWVDNPSK
                     LPDPARIDRIEEPFLLTQIVTPTDYIGPIMKLATERRGVYETTEYLSPKTVDMRFQMP
                     LSEIVFDFYDKLKSQTRGYASFDYEFAEYRPSKLVKLDILINGNPVDALSVIVHADKA
                     FAWGRNFCLKLKHLIPRQLFEVVIQGAIGSRVIAREVVRPLRKDVTAKCYGGDITRKR
                     KLLEKQREGKKRMKQVGSVEIPQEAFLAVLQMDPG"
     gene            15755..16501
                     /gene="lepB"
                     /locus_tag="PLT86_04915"
     CDS             15755..16501
                     /gene="lepB"
                     /locus_tag="PLT86_04915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT35985.1"
                     /translation="MDKPPVVPSSVVAGSGEKTATRKSLGKEYVQPLLTVLLVALVIR
                     TFVIHAYRIPSGSMEDTLLVGDFLLANRFVYGSPIEIPFTGIVLGRLPAISSPQRGDV
                     VIFASWADTTEDFIKRCVGLPGDTILVRNNVLTVNGTPFSQTLRARFGNDWRVFPSIK
                     YTPTHYLGMSGIMALDPTNFGPHVVPPGHMFVMGDNRNNSADSRFYGDVPLNRLKARA
                     LVIYWSMDTRQRLWKLSEYIRWDRIGRLIR"
     gene            16506..17210
                     /gene="lepB"
                     /locus_tag="PLT86_04920"
     CDS             16506..17210
                     /gene="lepB"
                     /locus_tag="PLT86_04920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT35986.1"
                     /translation="MQPDEPSETASKETTPWRVYAAVLSASLLGALLLRAFVIHAYRI
                     PSGSMESTLLVGDYLLAERLTFGSFVELPVARKELFRWPPLRRPRRGEVVIFRSWGDP
                     GTEIVKRCVGLPGDTVEVVDNIVKVNGVASDPIFQGYFDTTHKVNTEDVFPVSEWRAV
                     ERARNFGPHIVKPGHIFVMGDNRTNSEDSRVHGDVSFEALTGRPLFIYWSVNSHSASL
                     NPFDSIRWERIGKRVH"
     gene            17245..18636
                     /locus_tag="PLT86_04925"
     CDS             17245..18636
                     /locus_tag="PLT86_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT35987.1"
                     /translation="MTIPREMLEHLKELVGARFVLNHATEMLPYEADGLTEFRTPPAL
                     VVLPKTAEQVQECVKTCVRFGFPFTARGAGTGLSGGAVPAPGGVVISLSRMNRILEVN
                     YQDRTAIVEPGVLNLELSELTSPQGFVFMPDPASQPACTLGGNIGENAGGPHCLKYGV
                     MVNHVRALEVVTPTGDLVTLGGAGGDAVGYDLMGLFIGSEGTFGIATRLFLRLEPTPE
                     AVRTVLADYTSIAEACQTVSDIIAGGMVPTALEIMDRRTIGTVEASALAAGYPVDAEA
                     VLIIEVDGLPYSLDDQVDRITEICRKNGARSVRFASDESERKRLWAGRKAAFGASGRI
                     SPDLLVQDAVVPRTRLAEIVPRITEICDRHGVKVSNVFHAGDGNLHPNIGYDRRNTEE
                     ARRVKDALAEIMAICVATGGTITGEHGVGLEKVDFMPLYFDRATREAMLRVRSVWDPT
                     GLCNPGKVLSSQD"
     gene            complement(18653..>19085)
                     /locus_tag="PLT86_04930"
     CDS             complement(18653..>19085)
                     /locus_tag="PLT86_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35988.1"
                     /translation="ERLVDTEGKVTSLAFDRARGYLWIGTPSGLARYEAYPPVGAGDN
                     IIAEPSQNPFMIGISQRGSDYVLTGTPLSIVVTPGATVRIYTITGDLVWEATDSGIGQ
                     VTWDGRTRSPARAVASGVYLYIAQKGSNTATGKIAVLRDAR"
BASE COUNT         3899 a         5381 c         5687 g         4118 t
ORIGIN      
        1 cggaggcgtt cttctcctta ctgttgcgcg ctactatacg ccaaccggga gacttatcca
       61 gcgcgattac ggtgcgggca cagcgcagta cattttcagt gggattgccg aaggagtcag
      121 agtcgatacg tcgcagggac cgcgtttcgt aacacctctg ggacgagtag tttacggcaa
      181 aggcggcatc tcgcccgatt acgcgattcc gcggaatgag cctgatccag tcctcgcgaa
      241 gctttactct tcggcgctgt ttttccgatt cgtgaatgac gccgcgaata cagtagtcgt
      301 aacgccgcag cgaggggagg aattccgcgc ttcgcagctt gtggacgatc gcgtgctcgg
      361 tgagtttttc gatttttgtg agcgaatgga ttcctcgttc acaaaactga aactggaggc
      421 agaggagaac tccatacggc cccatctggc ggccgcgatc gcggagaaga cgtgggggca
      481 gcaaggtcgt taccgtgtcc ttgctccgtt cgactctgag ctttccgcag cccgtgcgca
      541 tcttgtagat gcacgatcgc tgctcaaccg ttctcgttct ggaagggcgc acattggacg
      601 ctgaagagaa cctttcaaac cctctttctt cggaggaaga ggagcaggta cgcgaggagg
      661 cgcgtaaacg ccttctggcc gagaacgaac accggaacgc aggcaacgga gttgccccga
      721 atggcgtcga cgtacacgct gagctgcgcc gcgcccatcg tcttcgcatc atgagggaag
      781 aagaagacgc cttctacgcg gagcgcggac tgttccgtta caggaaccat cggggagaga
      841 ccgaatggct cacccaggat gagatcgcaa agagaaaggc aaaccatcgc aggaaaaggt
      901 cgagtttgat ccggcgcctc cagcggcacc tgccatccgg ggagtttttc ggtttcttgg
      961 cggtcatcgg ccttctcgtc gcagggttcc tgtatttccg cgacgggcgc gctcctgcgt
     1021 ttcggactgc cctggagata agagtgatgt caaaccctca gggtgccgcc gtcttcctgg
     1081 acggggtgtc agcgaacgct acaaccgacg caattctccc cgtgcgaaaa gccgggcctc
     1141 acgtgatttc tgtcgcacgt ccgggttttc gggcagttcc ctcgtccgtc attgtgaacc
     1201 tcaccgaagg gtctcctcag gggctggcca cattcacgct gtatcctgtt cgttccgata
     1261 ccacaaacgg catggctgag taacgatgtc cggaccaact tcccttgctg aagaggcgtg
     1321 tgctcttttt cgggcgacct ggcagagggg gccggagtgc ctcgcgtcgt ctcctggccg
     1381 tgtcaacctg atcggtgagc atacagatta caacgacgga tttgtcttcc cggtggcgat
     1441 caacagacgc acgttcgtgg caatgggagc atcgcgcgac gggcgctcgg ggtttgtctc
     1501 cacctcgcac ccgattccag tactggccga ttgtccaccg tggcggcgca tggaaaatga
     1561 ttgctgggcc aactacgtgg caggcgtggc ggcccagttc gcgaatcgca acgaacgcgt
     1621 acctgcgttt cgcgcgtctg tggcaagcga tcttcccgcc ggtgcggggc tgagcagttc
     1681 ggccgctctg gaggccgcaa ccgctgccgc gttgagccag ttggtcgggg cctcgttttc
     1741 gataacggaa ctcgccgccc tttgccagaa agcagagcac gaattcgcag gcgtgcgttg
     1801 cggaataatg gatcagatgt caagcgttgc aggaggtgca atcgtccttg actgcagatc
     1861 acttgcgttt caacgcgtcc agattcccac ggacctgtca ctcgtcattc tcgactcggg
     1921 aatcccgcgc gggctctccg ccacgcgcta caacgagcgt agaggtcagt gtgaagaagg
     1981 agtacgcgtg ttgaaaggac gattttccgg gataaaggca ctcagggatg tcacgccggg
     2041 aatgctcgat gcctgccgaa aggacctgtc ggagacagtg tttcggaggt gccggcatgt
     2101 agtaaccgag aacgtacgcg tttccgcggc tgcgaaagcc ttcgccgaaa acgattttcc
     2161 agcagtcggg aagctgctcc aggagtccca cgtgagcatg cgggatgact acgagataag
     2221 tctccccgag attgatcttc tcgtggaaat cgcctcttcc accccgggat gttacggggc
     2281 gcgcctcacg ggagcgggtt tcgggggcgc tgtgatttct ttggtggaca caggttcggc
     2341 agacgtgttc gcggaggaag tcgtcgagga ataccgaaag aaaacgtgca aggctggaac
     2401 cgccatgcgc gttgttcctg acgaaggaag cagaaaagaa gaaccggtgt agtcaactga
     2461 aaaaagggaa aaaacgatgc cgacatggaa tgtgttcgtc acgcgccgta taccggacgc
     2521 aggtctcgat ctgctccgca acgcgggtgt gaacttcgaa gtcaacccgg aagaccgcgt
     2581 gctgacgcat gaggagttgt tagcgggtgt gagcggaagg gacggagttc tgtgcctgtt
     2641 gacagatatt gtggacgaat cggtcctgga agctgcccgc ggcgcgcgcg tgttcgcgaa
     2701 ctatgcggtg ggctacaaca acgtggacgt agccgccgct acgaaacgcg gcattatggt
     2761 aacaaacacg cccggcgtgc ttacggagac tactgcggac atggcctggg cgctcatcat
     2821 ggcgacggca cgcaggattg tcgaaggaga caggctcgtc cgcgccgggg agttccacgg
     2881 gtggggacca atgatgctgc ttggcatgga cgtattcgga agtacgcttg gaattgtcgg
     2941 cgccggacga atcggctgtg cggtggccag gcgggcgcag ggattcggga tgaaggtgct
     3001 ttacacagac tccgccccca acccggagat atcgagcctg aacgcgataa gggtggatct
     3061 cgacgagctt cttaaggcat ccgatatcgt taccctccat gtccccctgc tggaggaaac
     3121 gcgccatcta atcggcaagc gggaattcgc cctgatgaaa aatggcgcca tcctggttaa
     3181 cacatctcgc ggcccggtgg ttgatgagcg tgcgctggtg gaggccctga aatcgggttc
     3241 cattgcgggc gcggggttgg acgtgtacga agatgaaccg cagctcgctc ctggtttagc
     3301 ggactgcaaa aatgccgttc ttacgccgca tactgcaagt gccacaattg caactcggaa
     3361 caagatggct gtcatggcgg ccacgaatct gctggccgcc ctccgcggcg acacaccgcc
     3421 aaacctcgtc aatcccgagg ttctgaccgg ctgaacccgg tcaagcctga ctggaaggac
     3481 tgaagcatca aatggaaaaa ctcgtttcgt ttcagaacca cggtcaacaa atcgtgggca
     3541 ttctgcatcg ccccgatctg gacgggacag tacccggtgt ggtgctttgt cacgggttca
     3601 ccggaacaaa aagcgaagca cggtggatat tcgtccgtct cgcacggcgt ctcgcgagtt
     3661 cgggtattgc agtcctccgt ttcgatttcc ggggatccgg cgacagcgag ggcgagttcc
     3721 gcaacatgac gatttcggac gaggcctccg acgcgaaagc cgccgtaacc tttctctcaa
     3781 cgtgtgacgg ggtggatcgc gcgcgaatcg gcatccttgg attctcgctc ggagggtgtg
     3841 tagccgcgtg tgttgcgggg gatatcccgc tcttgaaagc ggtggtcctg tgggctcccg
     3901 taagcgaccc ggacgttcag ttcgcacctc ttctcaccga aggaatcacg ttcccacacg
     3961 agttctcgcc aggtttgttt ctggggcgag catttgtgga cgaattgcct cgcgttgatc
     4021 cggtcgcctc gctcgcccgt tcgagtgccc cggttctgat tcttcacggc accaatgacc
     4081 aggcggtgcc agccagcaca agtcaatcgt ttatcgatgc gcgggcttcg acgggaatcc
     4141 catccgaacg tgttctgatc gatggcgcag accacctttt cggcagtgcg ggcacggagg
     4201 aacaggtaat cgatcgcact gtttcgtggt tcgcacgtca cctggcggtg agccctgcgg
     4261 aaccgttgcg gacggtaacc tacgcggacg caggtgtgga tatcgaaaaa gcgaacagaa
     4321 cgaaggaact cctcaaagag cttgttcgca ggagttatac gccacaggta ctgaccgagc
     4381 tcggaagttt cggcggcttg tttgaggcgg atttcgggga cgtagaggac cctgttctcg
     4441 tcgcgagcac agacggagtg ggcacaaaac tcaaagtggc cgtaacggcg ggccgtcaca
     4501 ccactgtcgg gatggatctg gtgaaccatt gtgtgaacga tatcctcgtc atgggtgctc
     4561 gtccactctt cttcatggat tacatcgcgc tggggaggca cgatacgcag attgtcgtcg
     4621 acgtggtgga gggcttgtcc actgcgtgca ctgcagtcgg gtgcgctctg ctcgggggag
     4681 aaacggcgga gatgccggac atgtacaggc ccggggagta cgacctcgcg gggactatcg
     4741 tcggagttgt gggaaaacga gaagtcatca ccggcgctaa cgtgcgccat ggcgacgtgc
     4801 tgatcggctt gatgtcggac ggcctgcata caaacggata ttcgctcgca cggaagttga
     4861 tctttgatat cgccggctgg aacacagaca cattcgttcc ggaatggggc aggacggttg
     4921 cagacgcact tcttgcccca caccgatgtt atctccacgc acttttgccg ctgatcgcgc
     4981 gcggaatgat ccacgcaatg gctcacgtca ccggaggcgg cattacggac aacgtccctc
     5041 gaattctccc ggagggactt tccgcccgcg tgcggacggc agcgtgggac acaccaccga
     5101 ttttccggac tctggtcagg ctcgggaatc tcaacgcgga agaagcgttc cgggctttca
     5161 atatgggtat cggtatggtc ctcgtggtcg acagcacctc ggcggatact gtactcggag
     5221 aactaaccac agccggggaa acggcgtggt tgataggcga agtagtctcg ggggatcgga
     5281 acgttcagta tgtctgagac acctcagagc gaaccgaagt cagttctccg ccgatatcga
     5341 aagtcgcttc tgtccgtctg ggcgctgtac tcggccgcat tgaccgtcag catgatggtg
     5401 cccggcgaca gcgtaccaga cctcggattt ttcgggtggg acaagcttgc gcacctggtt
     5461 gtttttatcg ttcttgcctt cctgaccgcc ggggcatttc ttacaaggga ctgcgtcatc
     5521 acgatcgtga tgagctgggg ggtgttgctg gggtgcgcaa ccgagttcgc gcaggcgctt
     5581 gcgccgggac gtattccctc cgtcggagat gcggcggcga atctgatcgg aactgttttt
     5641 ggcgcgctgc tggtgaaacg gtatctcttg agggattgat caggcgggcg ggtttcgttc
     5701 ctcagggtga tgaatcgttc cgaagcgctt ctcgtatttc ttgatgttgt cgtcaagggc
     5761 gttcaaaagg cgctttgcat gctcgggcgt gatgattatc ctcgacacga ccttcggccc
     5821 gggcagaccc ggcacatgaa ccgagaaatc gaggattacc tcggtcgccg tgtgcgtaat
     5881 catgacgacg ttcgagtacc gtccttcgac gtgctccccg agattgactt ccaccgagtt
     5941 ttgcccgccg ttgggttctg taggtcctat catagtgatg aatgataccg tttcctcagc
     6001 caccacacaa gttgctccgg caccgcatgc gattgaaatg cgctcggtag cgttcgagat
     6061 cggcgggaaa gctcttcttg acggtgtgtc agtcacgatt tcgccgggtg agtatgtcgc
     6121 agttatgggg cgtaacggct cgggaaagac aacacttatt cggcatttca atggcctgct
     6181 ccttcccaca tcaggtgagt gcttcgcatt cgggctgccg acccgggacc cgcggtgcct
     6241 gcgcgaaatc aggcggcgaa tcggtctggt ttttcaggac cctgacgatc agatggtggc
     6301 tgtgacggtt gaagacgaga ttgcattcgg cttggagaac cgtgccgttc cccctgagga
     6361 aatggaccaa cgagtcgagg cggtattgga catcttccac ctccagcacg tcaggaaacg
     6421 ctcaacctcg gcgctttcgg gcggtgaaca gcaacgtgtc gcgattgcag cggtctgggc
     6481 gacaaacccc gacgttcttg tgctcgacga gcccacctcc atgctcgatc tcgtttcctc
     6541 tgtggcactg ttggagctcc ttaacgaaat ccggggttcc aaagcggtcg tccatgtaac
     6601 acagaacctt caggaagccg cgcgtgctga gcgtttgatc gtgatggatg gaggacgcat
     6661 cgtgttcgac ggtccgccgc agcaggcgtt ggaggatcgt gattccctgc gcgggtgggg
     6721 agtcgtcgcc gcgggcagaa cagcgtcgcg gaacgccccg ccagggaaat caatcgccat
     6781 ttctaccgaa gcactcaatc acgtgcgcga tgatgggaat gaaaccaacc acgtcttgaa
     6841 agacgtgacg ttccaggcgc tccgcgggag cgtgctcgcg atagtgggca aatcgggttc
     6901 cggaaagacg accctggcat atcatttcaa ccggttgctc gaaccgacca gcggagaagt
     6961 ccgtatagga gaacgacccg cccgcagcat tgctctgcat gagctacgac ggatcgtagg
     7021 attgacgttc cagagggtag acctgcagtt gttcgaagcc tctgtgctgg acgacgtggc
     7081 cttcggattg gtacaacgtg ggatggagag ggcggaggcg taccgacggg ctcgcctttt
     7141 cctgcaccgg atgggcctgg acccggacag tttcgggaat cgcgcgccgg gaacgctgag
     7201 tacaggagag aagcgccggg tggcactggc gggagtcctc gtctcagaac cggaggtaat
     7261 agtccttgat gaaccaacgt ccggacttga tgccgcaagt gtggaacagt tgtccgaaat
     7321 cgtgagcgag ttggcaaatc agggcaagac gctgctgata atcacgcacg atctcaattt
     7381 cgcggcgcgc acagccgacg gtgtcattct cgtggatgaa ggccgcgccg aggtgctgga
     7441 aggcacagat cgtcttggcg aactggctgc cacatggctc gcagaaacgt tgagccgttt
     7501 ccggtcttca gcggcttgac cctgaccgcg tcatcacatt cattcgggtt gtccgggaac
     7561 gcggtcccac caacgatcga acgcgggtac ggtcttgctg cgaattgtct ccctccttct
     7621 ttcattcgcc tccgcgttga tggcgttctc gtgtggagca atgcgcgcga gagacaatga
     7681 caagctcacc atcgtgtggt acgggcttgt aagaaatgcc tccaccgtga accctgtgga
     7741 ccgaatgctg cgggatggcg ccacggtttc gcccggggag ggaatccaac ttttcgtttc
     7801 ggtcaaaccg gcggcgttcc tctatgtcat ccaccagtcc ccgacaggac ttctgactgt
     7861 ctggtggccc tcacagagtt cgggattcaa tgcgtaccta gaaaaagaac gcgttctgac
     7921 acttccggcg cgcgggaaaa ttcacgtgct gggagccgcc cagggaacgg aggcggtgta
     7981 tttgattgcg gccttcaaac ctgtcacccg cgtggacaag ctgacgaagg aaatccaatg
     8041 gctgttcgct tccgcccagg ccttcggttc aggggtgccg gcgcgacagg taaaacagat
     8101 gcttccaagg ggtcttgtct ggcgacttga tgcaaatact ccggtaggcc ttaccgacca
     8161 gcgagttcct gtcgctacca cagttcggtg tatcgggaga atcgccgggg gcggcactgc
     8221 cacgagcact tccgactcgc aaaataccgt gattccggaa caggtcagag gtggcgacgt
     8281 cgtcgcacga gtaatccgat tccagcgtca gttgcgctga attcatgccc ggatgcgcgt
     8341 gaattccttt ctcgtggcac cgggttcccg gacgaccacc gcgccgatat cgtagcaatc
     8401 gtacacggca gtgacggact tgtcagccgc gatccgctcc cacgccctgg ccattcccga
     8461 agaccaccgg atgtcgtcaa acaccagaat ggccccttcc gacgaggctt cgatgatcat
     8521 tccagcgtaa tccagcgtcg ccctttcgtc gtggtggccg tcgacaaacg cgaaatctac
     8581 actgcccgcc tctttcaacg tgtccggcaa gcctgtttcg aaactcgagt ttactatctg
     8641 aacattgtca tatcccagca gtgtcatgtt tctcttcgcg cattccgtga gcgagggaga
     8701 tccctcgatg gtaaacaggc gccccgatcc gttcagtttg agtgcagcag caaggtatgc
     8761 ggaagagatc cccagattcg tgccaagttc gatgcagttg gcaggcttcg tttttcgaac
     8821 aaggcggaaa agaacccggc accaccgcgc gggcacactg gagtaccgac actcatgcgc
     8881 cactcgggta acccgtgaag ccacggcttc gccagacccg aagtggaaat ccgaaatggt
     8941 agccgtggag ctgagaagtt ccttgcgcag gccttcaatc cgtccaactt caggtccccg
     9001 ctcttcctca agcggtgcga aaaggtgttc tggaggtagt tttgcggcgt cgtgcgcgcg
     9061 tagcacatga cccagccggc caaagcgccg acggaatgcg aagccggaca tccctccgtt
     9121 acctttcccc gggtcttgcg gtgcgcgtgc gtggcggtgc agaaccatcg ctcatgcggc
     9181 cgacgtctca gtttccttca ggctgcctcg aagaatctcg cggacttcgt ccccctgcag
     9241 gttttctcgt tcgatcagcg catttgcaag ccggtgtaac gcatccgtgt tgttttcaag
     9301 gagttctcga actcgttgat atccttcaga cacaattctt cgcacttcgt catctatctc
     9361 cgccgcagtt tcttcgctgt agtgcaccgc gcccccgccg accggttgcg cgaggaatcc
     9421 gggagcttcc ccgtatgaca ccagcccgag acggctcata ccgtaatcct tcaccatgtg
     9481 ctgcgcgatg cgggtggcac gacggaaatc cgttcccgca cttgttgata cgtcaccaaa
     9541 cacaatctct tcggctacgc ggccggcaag cgcaaccgca aggcggtcct ccagctcctc
     9601 cttggacatg agatacctgt cttccgtcgg ttggtacgta gtctggccga gagaggttac
     9661 acctcgcggt atgatcgaaa cacgatgcac cgggtcagtg tgaggcaggg cttccgcgac
     9721 cagcgcgtgt cccgcttcgt ggaacgcagt gatgcgtcgc tccgtctcat tcagcacgcg
     9781 gctcttccgc tccaaccccg cgatgccacg gtcaatggcc tcgttcaagt cttccatggt
     9841 taccgcaggg tggttcttcc gggcagcgag aagcgcggcc tcgttgatca tattggcgag
     9901 gtcggcgccc accatacccg gggtccgcgc ggcgatctga tgcagatcaa catccggagc
     9961 cagtgttacc ttacgggcgt gcacgcgcag tatctcttca cggcctttga tacccgggcg
    10021 gtccaccaca atctggcgat caaaacggcc gggcctgagc aatgcctggt caagggtttc
    10081 cgggcggttt gtggcagcca tgatgatgac acccgtgttg tcatcgaaac cgtccatctc
    10141 cgtgagaagc tgattcagtg tttgttcgcg ttcgtcgtgt ccaccaaggg gattcacccc
    10201 gcgcgctttg cccagagcgt cgagttcgtc gatgaaaacg atgcaggggg atcttccttt
    10261 ggcctgttga aacagatcac gcacccgggc cgctccaacc cccacaaaca tctcgacgaa
    10321 atccgacccg gagagactga agaacggaac tcctgactcc cctgccactg ccctggccag
    10381 aagcgttttg cccgttccgg gaggaccgac aagcagaacg cctttcggaa tgcgaccgcc
    10441 gagtttctgg aaccgcgccg gttctttgag gaactctacg acttcccgta cttcctcggc
    10501 ggcttcatcg ataccggcga cgtcggcaaa agtaaccttt gtctccgttt ccacatacac
    10561 tttcgctttg cttttcccta tggacatcgc tccgctggat ggacctccca tgcgacgcaa
    10621 aaagaaaatc cagaacccga tgaggacaac catgggaagg acccaggaga gaattttcgg
    10681 ccacgggccg gggataaccc cgcggtagga gatgccgtgc tcgtcgagca gagaaacaag
    10741 ccccttgtcg tcctttatcc tcaccacgtt gaacgatcgg acggaatcac cggcgccaac
    10801 tattcgcccc tcaatctcgt cttctccaat cgccactgct gttactttgc gttcgcgaac
    10861 agcctggatg aattccgtgt agaacagcgt tttgctggga ggttttgagt attgccggat
    10921 cgccagcacg atcatgaccg cgagcaccgc gtaccagaga gagaacttca ggcgcccgga
    10981 cacagatggt cgtcttctgc ggggttccga aggtttgggc ctcggatctg gtttttcaga
    11041 atccacgaca acctcgaggg gttccaattc agctagacgg attcgcgcca cggctgcggc
    11101 aacgggcacc taaaaagata gcaggtgcga gcagttatga gaacgtctgc aggtgtgccg
    11161 cccgaatcga tcggatgatg ccgtggtcag tggaaccggt tttgagtacc atataggcgg
    11221 ctgtgtgacg gctcccgccc gaagacaaaa taaggaggtc ggcaattgtc agaaggcctg
    11281 tgggcttccg ctcgttccag attgtggtgt aacgacacgc ggttggcgga aagcacctgg
    11341 ggcagagtac tcttgcgact cctgcgcacc agcgtgatcg ccggcaaggg gttctggaac
    11401 gacgaatgca tgctgcgggc gactgcactg gcatacacca cgctcctttc cctggttccg
    11461 cttctggctg tggcgttttc acttttcgca gctttcccgg agcttgagaa tgcctacgcc
    11521 gaaatacgct cctttctgaa cgagtacctg gcaccgggag caagcaaggc cgccatttcc
    11581 gcactgaacg aattcatagc gaatgcaacc accggtacgc tcgcgggaat cggaactgcg
    11641 gcacttttcg tcaccgtgtt tctcatgctg agctccgtcg aggagtcatt gaaccggatc
    11701 tggcgggcgc ccaagacacg accgattctg gacaggctca tctactacct ggcaatcgtc
    11761 atcctcggcc ctctcttcct cgcggtatct gtgaacgccg cgattcaaac ggttcaacgg
    11821 gaatcactgg cgctcaatct ggtcgagcct ccattcgtgt ccgagttgtg ggagtattcg
    11881 ctgccgctcc ttgcatcctg cgcgctgttt acgatactgt tcaaagcgat gccgaatacg
    11941 aaagtgcggt ggccagcggc ggttgccggg ggaatcgtct ccggggtgct gtttgaaatc
    12001 gccaaaaagg cgtacaccct gtacgcgtca aacgccatcg cgtacaggac tatctacggc
    12061 gcgatgagta cagtgccgct gttccttgtg tggatatacg taatatggct cgtcgttctt
    12121 tttggcgcgg agttcgccca tggttggcaa actataatca gctatcagag aatcgcaaca
    12181 tggggcccga caagtcagta ccatcgagag cttctggctg cagcgctcgc tgttgccacc
    12241 accagaaggt ttgaggaagg caaaccgggt ctgacgtcac aggaaatggc agacatcttt
    12301 gatgcgccgc tcgggattgt tgaggagatc gtcgagatcc tcaaatcgag ttcagttctc
    12361 acggaatcag ggcgcggtat ccttccggcg aaggacccgg acaagctgac actctgggac
    12421 gtggtcgggc ccttgaggaa aggtgcggag accaggccga ttcccgagca gctttttcgt
    12481 gaggaggtcg gccccgctgt tctgttcctc gaagaggtcg agaccggcaa cgaagaaccg
    12541 tttcgatcca ttacgcttgc ggaacttgca gccaaatctg ccgttgcaga aactactcct
    12601 gacgaacgcg gacaatgacg gtgttgcgcg gtcgtctccc cgcttgtatt attagtattc
    12661 cgggcaaacc agaacttcac ctttggtagt aatcattgca cgtcaccctc ttttggagga
    12721 cgatccgtgg aaaacttggt gagtaacctt ctgagttggg tctcttcgtt cttcgggaac
    12781 ctgtacgccg atgccccgat catctgggga tcgaagtacc ctgcgtttct cggggaactg
    12841 cttctgctta tcatcgtgat cgacttggtg attttcgggt ggcggccgtt ggtgaagagg
    12901 tttttcccca agtcctatgg cgccgtcgat tacgcgttcg ggcaggtgac aaacctgatc
    12961 tggctgtgca ttcttgttgc gttcatgatt tatatcggcg gtgcgctcgg ggaagcctgg
    13021 ggcagatttc ttggcgtatc cacaatgatc tggtcgacgg tcggcgtggt tgctctcctc
    13081 ctcctgggca aactggcagt gaagcggcac aggtagccgg cagcgggaat acgcacgttc
    13141 catacaagga aaccggtggc ttcactaccc ccaccggttt tttgttcccc ttttggcgct
    13201 gacaaccacg ctcgttatcc catccctggt gtgcctatgg gtgtcgttgc cacttttttc
    13261 atagcgctcg gactttccgc ggacgcgttt gctgttgccg tttcgaacgg attttcgtcc
    13321 cgcagaccgc ggattggcga agcgtttcgt ttcggcgtcg ccttcggatc gtttcaggca
    13381 ctcatgcctt tgataggatg gctcgcaggc tcgagcatgg caggtttgtt cgaggatact
    13441 gaccactggg ttgcattcgg cctccttgcg ttcatcggcg ggaaaatggt gtacgaggga
    13501 atctggggag aggcgacgaa cggcgaggga aagcccgtga ctttatgggt gttgctgatt
    13561 ctgtcggtag ccacaagcat agatgccctg gcagtgggaa taagtctttc gttgctcagc
    13621 acgccgatat tcatgcccgc gatgcttatc ggcgttacga cattatgcgt gtccactgcc
    13681 ggcttccttc tggggaaacg cctcggtcgc ttcttcgggc ggagagttga ggtcgcggga
    13741 ggcattatcc tggtcgcgat cggcgcgaag atccttatcg atcatttgac acgtggtgtc
    13801 ggagggcagg tctgaaccac atgcgacacg tgtatccgac ggttctggcc cgcttttttg
    13861 tatctttcgg atgtctcctt tcaggtatcc cttcatcagt atgttctgcg aacacccgca
    13921 tgggaaaaga atgacagacc agaaacgcat tcgaaatttc tccataattg cccacataga
    13981 ccacggtaaa tcaaccctcg cagaccgcct gcttgaattc accggcacgc tttcctcaaa
    14041 tgagatgcag gatcaggttc tggacagcat ggacctggaa cgggagcgcg gcatcacaat
    14101 caagatgcat gcaatccgga tggaatatgg tgccgagaac ggcgagacgt acgtcctgaa
    14161 cctcatcgac accccgggac acgtggattt ctcgtacgag gtgtcacgca gccttgcggc
    14221 ctgcgagggt gcgatcctcg ttgtcgacgc tgctcaaggt gtggaagcac agaccgtcag
    14281 caatctttac caggcgatct ccgcggacct tgagatcata ccggtaatca acaagatcga
    14341 tttgccaagc gcacagattc cgctcgttac tcaggaaatc agggatcttc tcggtgtcga
    14401 agagaaagac atactcctgg tttcggcgaa aacagggcag ggaatccgcg agctgcttga
    14461 agccatcgtt gcccatgtcc ccgaacccaa aggtgatccg gacgcgccat tgcgtgctct
    14521 catattcgac tctgtctacg attcttaccg cggcgccgta gtgtatgtcc gtgtcgtgga
    14581 tggggcgatt cgccccggac agaaggttcg acttgccgca acgaacgccg ttcacgatgt
    14641 cgacgaggtc ggcttcctga ggctcggacg cgtggcccgc gattcccttc aggctggtga
    14701 agtggggttc gtggtcgcgt cgatcaaaac gatcagcgag gcacgggtag gcgacacgat
    14761 cctggacaac tccgctcccg cggttgttcc gctgaagggc taccaggaag tcaaacccat
    14821 gctgtttgcg ggcatctatc ccgtggacgc ggaaatgtac gaggatctgc gggattccct
    14881 cgctcgtctc aaactcaacg atgcgtcgct cgtgtacgaa ccggaaagct ctgctgctct
    14941 gggttttggc ttccgttgcg ggttcctcgg tctcttgcat atggagatcg ttcaggaacg
    15001 actcgaccgg gagttcggcc tgaacatcat cacgaccgtc ccaaacgtgc ggtaccgcgt
    15061 tgttacgacg tccgacgagg taatctgggt ggacaatccc tccaaattgc cggatccggc
    15121 tcgtattgac cgaatagaag agccgttttt gctcacgcag atcgttacgc caacggacta
    15181 tatcggcccc atcatgaaac tcgcgaccga aagacgcggc gtgtacgaga caacggagta
    15241 cctttcgcca aaaaccgttg atatgcgatt ccagatgccc ctttccgaaa tcgtgtttga
    15301 tttctacgac aagctgaaat cccagaccag agggtatgct tctttcgact acgagtttgc
    15361 agagtaccgc ccgtcgaaac tcgtcaagct ggacatcctg ataaatggaa atcccgttga
    15421 tgcgctgagt gtcattgtgc atgcggacaa agcgttcgcg tggggaagga atttttgcct
    15481 gaaactgaag catctgatcc cgcgtcaatt gttcgaagtg gtcattcaag gagcgatcgg
    15541 cagccgcgtg atcgcccgtg aagtagtccg gccacttcga aaagatgtca cggcgaagtg
    15601 ttacggcggg gatatcacac gtaagcgcaa actcctcgaa aagcaaagag aaggcaaaaa
    15661 aaggatgaaa caggtcggaa gtgtagagat tccccaggaa gctttcctcg cagtccttca
    15721 gatggacccc ggttgaaaag agaaggagca ggcagtggac aaaccaccag tggtccccag
    15781 cagcgttgtg gctggttcgg gggaaaaaac ggcaaccagg aaatcccttg gcaaggaata
    15841 cgtacagcct ttgcttaccg tgcttctggt cgctctggtg atacgaacgt tcgtgatcca
    15901 cgcataccgc attccatccg gatcgatgga ggacactctt ctggtcgggg atttcctcct
    15961 tgcgaaccgg ttcgtgtacg gctccccgat cgagatcccc ttcaccggaa tcgtgctggg
    16021 aagactacct gcaatatcat ctccgcagcg cggagacgtg gtgattttcg cttcctgggc
    16081 ggatacgacc gaagacttca tcaaacggtg cgttgggtta cccggggata cgattctcgt
    16141 caggaacaac gttctcacgg tcaatggcac gcccttcagc cagaccctga gagcacgctt
    16201 cggcaacgat tggcgtgtct ttccgagcat caagtataca ccgacgcatt atctcggaat
    16261 gtccggcatc atggccctgg atccgaccaa ctttggtccg cacgtcgttc cacccgggca
    16321 catgtttgtc atgggcgaca acagaaacaa cagcgctgat agtcgcttct acggcgacgt
    16381 cccgctcaac cggctcaagg cgcgtgcgct tgtgatttac tggtccatgg atacccgcca
    16441 acggctgtgg aaactctccg aatacattcg ctgggatcga atcggcagac tgatccgctg
    16501 accccatgca accagacgaa ccctctgaga cggcgtccaa agagacaaca ccctggcggg
    16561 tttatgcagc ggttctttct gcgtcgctcc ttggcgcatt gttgcttcgc gccttcgtca
    16621 tacacgcgta ccgcatccct tccggctcca tggaaagcac actgcttgtt ggcgattacc
    16681 tcctggcgga gcgtctgaca ttcggctcat ttgtggaatt gcccgtcgcg aggaaggagc
    16741 tgtttcgctg gccgcccctg cggcgaccga gacggggtga ggtcgtcatc tttcgctcgt
    16801 ggggcgaccc cggaaccgaa atcgtgaaac ggtgcgtggg tctgccgggg gacacggttg
    16861 aggtggtaga caacatcgtg aaggtgaacg gggtagcgtc ggaccccatt ttccagggct
    16921 acttcgacac gacgcacaag gtcaacacag aagatgtttt cccggtgtcg gagtggcggg
    16981 cagtagaacg tgcgcgcaat ttcgggccgc acatcgtcaa acccggccac atttttgtga
    17041 tgggcgacaa ccgtacaaac tccgaggaca gccgcgtcca tggggatgtt tccttcgaag
    17101 ctttgaccgg gcggccgttg ttcatttact ggtcagtcaa ctcgcattcc gcatcactga
    17161 atcctttcga ttccatcaga tgggaaagaa taggcaaacg tgtccattga aacgctcacg
    17221 acaactttcg cggaaggacg cgccgtgacg attcccaggg aaatgctgga gcacctcaaa
    17281 gaactggtgg gggcaagatt tgttctcaac cacgccacgg aaatgttacc ctacgaagcc
    17341 gatgggttga ccgaattccg cactccaccc gcgcttgtcg tgctcccgaa aaccgcagaa
    17401 caggttcagg aatgcgtgaa aacgtgcgtc cggttcggct tccccttcac ggcacgtgga
    17461 gcaggaacgg ggttgagcgg aggagcggtt ccggctccgg gaggcgtagt gatctctctc
    17521 agccgcatga accgcatcct ggaggtgaat taccaggacc gaacggcaat agtcgaaccg
    17581 ggagtcctca acctcgagct cagtgagcta acctccccgc aaggattcgt attcatgccg
    17641 gatcccgcga gccagcccgc ttgcacactc ggtggcaaca tcggagagaa cgcgggcggc
    17701 ccgcattgtc tgaaatacgg ggtcatggtg aaccatgtgc gggcgctgga ggtggtaacc
    17761 ccgacaggtg acctggtgac actcggaggc gccggagggg atgccgtcgg atacgacctt
    17821 atgggcctct tcatcggctc cgaaggcact tttggaatcg ctacccggtt gtttttgcgt
    17881 cttgagccca cccctgaagc tgtcaggacc gttctcgccg actatacgtc aatcgcagaa
    17941 gcgtgtcaga cggtttccga catcatagcc ggaggaatgg ttccaactgc attggagatc
    18001 atggaccgcc gaacaatcgg taccgtggag gcatcggcac tcgcggcggg gtatcctgtc
    18061 gatgcggaag cggtgctgat cattgaggtc gacgggttgc cgtacagcct tgatgatcag
    18121 gtggacagga tcacggaaat ctgtcggaag aatggcgctc gcagcgtacg gttcgcatcg
    18181 gacgaatcag aacgaaaacg cctctgggcg ggcaggaagg ccgcttttgg cgcgtcgggg
    18241 cgcatcagcc ccgatctact ggtgcaggac gccgtagtgc cgcgtacgcg gctcgccgag
    18301 attgtgcctc ggattactga gatctgcgac cggcacggcg tgaaggtctc caacgtgttt
    18361 catgctggcg acgggaatct ccatcccaac atcgggtatg acaggcggaa cacggaagag
    18421 gcgcggagag tgaaggacgc gcttgccgaa atcatggcaa tctgcgtggc aaccggcggt
    18481 acaatcaccg gcgaacacgg agtgggtctg gaaaaggtcg atttcatgcc tctgtacttc
    18541 gaccgagcta cccgggaagc catgttgcgc gtgcgatccg tctgggatcc aacggggctc
    18601 tgcaaccccg gaaaggttct ttccagtcag gattgacggc ggcacgcgca gttcagcggg
    18661 cgtctcgcag gacggcaatc ttgccggtgg ccgtgttcga tcccttctgc gcgatataaa
    18721 gatagacgcc ggaagcaact gcccgggcgg gtgaccgcgt tcttccgtcc cacgttacct
    18781 gcccgatgcc agaatccgtc gcctcccaca ccagatcccc cgtaatggtg taaattctta
    18841 ccgtcgcacc gggcgtgacg acgatcgaga gcggcgtgcc cgtaagcacg tagtccgagc
    18901 cccgctggga aattccaatc atgaaggggt tctgcgaggg ttccgcaatg atgttgtcgc
    18961 ctgcgccaac cggggggtac gcttcgtacc tcgcgaggcc ggaaggcgtt ccgatccaca
    19021 gatagccccg tgcccggtca aatgccagtg aggtaacctt tccctctgta tcgacaaggc
    19081 gttcg
//
LOCUS       DAOSOO010000043        18966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2916_length_18966_cov_5.508487, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000043 DAOSOO010000000
VERSION     DAOSOO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_18966_cov_5.508487"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PLT86_04935"
     CDS             complement(<1..178)
                     /locus_tag="PLT86_04935"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT35989.1"
                     /translation="MTYEGQLSAEGHRFGIVVSRFNAFISEHLLTGCLDAIKRHGGDL
                     DKVDVVWVPGSFEIG"
     gene            complement(273..1502)
                     /locus_tag="PLT86_04940"
     CDS             complement(273..1502)
                     /locus_tag="PLT86_04940"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOT35990.1"
                     /translation="MIAVDEAGVFATIGEALDELRAGRMVIVVDDEDRENEGDLVMSA
                     QMATPDAINFMVTHARGLVCLAAEQSRLQQLGLELMVPSPNNTAAHGTAFTVSIDAVH
                     GTTTGISAGDRATTIRKFVDPAAKPQDFAKPGHVFPLAAAKGGVLRRAGHTEAAVDLA
                     RLAGHTPAGVICEILNDDGTMARVPELVLMAKRMGLRIVTIKDLIEHRRRSECLIRRV
                     EEVQMPTEYGDFLLILYESLLDQEFHIALVKGDVRTEDPVLVRVHSQCLTGDVFGSAR
                     CDCGAQLAWALRKIEAEGRGAVVYMRQEGRGIGLENKLHAYSLQEIGLDTVQANQFLG
                     FPPDIRDYGIGAQILRDLGIQRVRLMTNNPAKRAGIEGYGLDVVEMIPILIPPNPHNE
                     RYLHTKRTKLGHLLEYE"
     gene            complement(1499..2158)
                     /locus_tag="PLT86_04945"
     CDS             complement(1499..2158)
                     /locus_tag="PLT86_04945"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT35991.1"
                     /translation="MFTGIVEAVGTVTRVVRSGGVQHTEVACPEVMDDVALGDSIAVE
                     GVCQTVTDRTADTFTFDSVAETLRLTTIGSWRPGRRVNIERSLKVGARLGGHWVLGHV
                     DGVVRLLERKNLGDTIEIRCSLPSQLLPQVALKGSVALDGVSLTVTSVNDSSFGVAVI
                     PFTLEHTTLGGRKTGDEMHLETDVLAKYVSRLLESATPGGKASDRNIGALLREAGFMD
                     T"
     gene            complement(2158..3249)
                     /gene="ribD"
                     /locus_tag="PLT86_04950"
     CDS             complement(2158..3249)
                     /gene="ribD"
                     /locus_tag="PLT86_04950"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT35992.1"
                     /translation="MRERQEARFMDEALALAAKGRGYTSPNPMVGAVVVKNGTVIGRG
                     YHRQAGLAHAEVVALDEAGEAARGATMYVNLEPCSHHGRTPPCTDRIIAAGIERLVCS
                     HIDPDERVNGRGIELLRKAGIDVQVGLREREARRLNEHFLHWKTTGTPWLTLKWAQSL
                     DGRVATSTGNSQWITGEEARTHAHRLRSWHDAVLIGVGTALADDPRLTVRMVEGHQPY
                     HVVLDPGLRLSPNARLVEPGNTVILCAESADPQRIAAWERNDVQAVPVRSHGDVLEIE
                     AVLEALTALRFQSVFVEGGSRTLTSFLKAGAANRLTVFIAPIILGEGIPAVGDLGIET
                     LDQAIRLREVEVERFGNDLCITGLCGAGP"
     regulatory      complement(3464..3602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3637..5394
                     /locus_tag="PLT86_04955"
     CDS             3637..5394
                     /locus_tag="PLT86_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOT35993.1"
                     /translation="MSVNVRMIEAGSDLVWLCGMSRELETAFAGRKVTLYRHRTRPAC
                     LLEAGGARICVADLTPGSSQIQNDYPVVREEWRQWGTRRTDGAEVEHVRVRDGDRVIS
                     IHLVWRNRIGDEERTELIWELGGLRTNAILVSLDGTVLDVLRPVSERVNRVRTVLPGR
                     PYVPPPTGVRQRLDEPWTWDAGDGADTGGNGPTVHEFLARGFLASSGFLADELLARCG
                     VDGALPVAQCGRETRVRIESEFRNLLADPSPHLLLDESGEPAGYCGYRPTHVAPEQVA
                     PAGSFCEAATRTWRARRESQPGSDAILDQLATWRGALRKLLRQRDNLRGDVVRAEQAG
                     ELRRLADLIMANLSDIPEGTEVVTLTDWFQDSGATVQVRLDPTRNAASQAEQLYRRAK
                     RLQNGLPLITARLRKTELAINSLEQQIREAEDAAGGSNDQRVRRYQMQGVSDGGGRKQ
                     PFQGIRPRRYRTRDGGWLVLVGRNDDENDVLSLKVAAPDDLWFHAQGCPGSHVVLKKE
                     GRKEQPGRQAIAEAAAVAAYWSKARGSSKVGVSYTLGKYVTKPRGSPPGTVTIRHEKL
                     ITVPPALLPLADEAQEATQ"
     gene            5391..10313
                     /locus_tag="PLT86_04960"
     CDS             5391..10313
                     /locus_tag="PLT86_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT35994.1"
                     /translation="MTARRIVFALFRGVGVLAALLVIAATLGYLFVTSPVGTRWAAHT
                     IKSQARSLAGVEADIGGLDTDIFTYVNLRRVHVALPDSGPSLQFERLQLRMALWRVVT
                     GRVNGIRRIAADSLVVTVPLKPDTAKFVWSVVPYLPYVPEDADIRGFRLRLLSATDTM
                     SFALKAVQLRSIPDQQDSSLYADINLHGEVTLKPLGTLPFAGKANLFFRGASLRIGNL
                     VVSGPGFLAKGHGYLDDLVNGPYQTHGSFVVDLARLPSEASGVEMSGTVNAEGDIGWD
                     GHPRINMKLESATISTPWAALANTTLFLNTSETDSTLSLRGATSVAEGNVGLSATMVV
                     PPASGPLTVDFAAVDVDLDSVARIPAVKEALAGTELRGEASVRWQYSAHASIAGGFKL
                     SQQVSRIELSTKGFQYGTMPIGTTSLVWNQTGPDVEGVFHGAGTSVHVTGVAKNLDNV
                     ALNANLRTPHLDSLLQVFGVDSLDGDLDVQTTITGALRAPTVGIVATLRNPVNPWMRL
                     PDLQLNGRMARGDTVSATLTSVDSVLRADAVIVNKFMRFASLRASIGPLPFEQLPGGF
                     AKEQDITGSLSADIVGAGLLTNPYLRGSIRTTPVAWHGQQIGTIRGDLNWFGNSLTLD
                     VRNGNDTFSAQGRVAADSGATSQIDVQWENFDLGGILSALTDQPAGKISGMTDGTISA
                     RWTRLVVNSLVIQARLDRASLRLGQATYELADPPSRFSMEKGVLDLTPTLFRGSNDQR
                     LHLQGSISRSGEMSFRAALDSFDVGNAISTLTDGKLAISGKMVLRAHLGGTLEEPVAN
                     GRVTFRGIRWKTVEIDSAGALATYSSNQLSVPEFSFRFPFGEARGSFRATASALGLAD
                     TATGPPDFALSLVMKDAGAVVPNWEGLRSGTVSISGELALRGRDLADISSYRGSFRLD
                     SLAAVAPFNRLVRTKRPATVAIGESRYLASPLDLAMSYRGAETGEISISQPPGVARPP
                     LDVSINRVSLEDFRHTILPLLNALDAQTSVPEDLSGEFSGHARWDMNLSSPRVDMSFL
                     IREPTAYSLRGDSLDCVAAVRDSGVWVESANFYTAGDTLNVTGFVDLSADTLLFHAYT
                     ANLELVPVLTDLLPPLRTYQPPPPLPTTAPFPGFPQQIRALPAEARGATVNAPLTTFD
                     RLVRARRGAMKHPYAATLPMAAEFELRGTLEFPLLFGNAQLFGAYLEIEALQEPIWLP
                     DTLSIRVTGKRAVVPPVRVFVGAQQRETQTPPAISLERAEYSLSRGEFILEAKIERAT
                     FSLVSTEPVFLPPRWRLFKGVLEPLLRAYYTEPIGVFTASATGSWRGTMEHSNLNADV
                     VLYSSTLQYPIADPQAIFKPRAPTSLGPLLDNTELNISLSMEDSLVINNNLSKHATLW
                     AQMNLTGRHYSPNLQGNVQISPGSVFRYLGRDFVIQEATVNFPDPTRFAPEIEVTARA
                     KVVNTKDNNAPSYDVTLHASGVVPNLVKTEMTAEELPSGEVIVNQLEIMQILIYGQRS
                     PDYLAIDAQGRLNTMLKERGYATLSSALGSVLPIDQVTVRESADNGAQQGTGGMNDVE
                     VEVGQNVRVWGQKLTFSAVSPVGQLSSVSGLERAEVRWTILERPPRWPNLESLSLTVG
                     QNRLQQAQASTDLYLQNELTSGLQLRVRFR"
     gene            10360..12342
                     /locus_tag="PLT86_04965"
     CDS             10360..12342
                     /locus_tag="PLT86_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT35995.1"
                     /translation="MRKRETKRIARFAALMALAILSMPQAVGAARSTFVQEIIITSTT
                     VPAPVGDEKWRQVPERRKLNGILQHPTPSFRNLWLPVRFDDVLFAADTMSVLTYYRNL
                     GYLGAYVATTAQEPVGGRLEGLQYVNLLIVIGGITPEERYTLHSIEFSGVTSLDTAAL
                     AKEFRKRHNAGKFFSPSGAAENFVALRTAYADAGYLDSNAVTIRQMPIVDTLARSVRE
                     RYEVDERTPTTVTGYAIINGNAPLPLITDSAVVVQALKDAKLVPGSVFGRQRMLDAES
                     NLFDLGVFRRARVAPDTAFARGSPFQRRAMVDIAERDAGDVRVQLGYSNIQQWRISET
                     ATYSNFLGEARLIGQEGQLSRDVQALSFMYGQPRIGVPSFVPLVGETSMRLRLDQALS
                     GSWEVLAAGDTSRTFAWKTSLSRRFGRMARIGISFDLSRSDTTSRFLFSRPRSGATLQ
                     SAVSVNALYDTRDEFLNPSRGFALNAQVRVVNPSHRVSRMSMRPEALAVYYRPVSPFV
                     IGAVSLGGGFYYVGKDDSLNQAEQFWRSRQMPTVRGFVRDDITVIRGDSVGSPAVAYL
                     LLKTEARIALWKRLSSAVFVDAGQAWVWIPRAVRNPTAEERAFRGWSAMEAGRFALSA
                     GFGPRLNWGLMFRLDLVRQITPSTHWTYEFGITQAF"
     gene            complement(12414..13694)
                     /locus_tag="PLT86_04970"
     CDS             complement(12414..13694)
                     /locus_tag="PLT86_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT35996.1"
                     /translation="MSELYQRIAAAVDAIPLFDTHEHFYDEATRLNVKKEVDLFYFLH
                     YVECDIKSAGSPDEAGEFAGGTLSSDEKRRRFLKYWPYVQDTGYGRAFRIMANDLWDV
                     PEINEGTYDLLADRATNFARPGFYKTVLRDRANVARSIRIVWAGQNTLCDLDSLFPAP
                     VFDNFVGPTSRADIRKLEAQTDTRIHSLGDLETALDAAFDRRQNEGMVAVKIFLAYRR
                     NLDFAHTTTAAAEAALQRVLVALPPSPLGVDETKPLQDYMVHQIVQRAVERKLPVKIH
                     TGYQNSNDNVFANGKPEPLINLVMSYPTARFVFLHCGWPYTDQVLAFAKMFPNVFPDM
                     AWAHILAPGMAQRLLHDLLDTVPSNKILGFGGDYNFAEGAYAHAKLARAGISKVLAKR
                     VEEGVFTEEQALVIAKRIMHDNAAALYGFPSLDA"
     gene            13885..14817
                     /locus_tag="PLT86_04975"
     CDS             13885..14817
                     /locus_tag="PLT86_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT35997.1"
                     /translation="MALKTGRAEGSATVTIDRVLCDACGCCVRVCSADVLRMENGAVI
                     ADTTRLFGCVGCSHCMLVCPTDAIAVHGRDTSLADVRELPPPEMRADFDSLLALLLSR
                     RSVRRFRSSEVGEEAIQKIVEAVSTAPMGLPPSDVEILVLRGRPRVQEFAGDIVDAIK
                     RAQWFVSPLMTTLMRRFIGKEAAEMFRTFLRPLAHALPEKRARGEDWLLYDAPLAMYF
                     HTSPYSDPADPVIAATYAMLAAESLGLGSCMIGTVAPILKYYKKVAEKYGIPPKNQQG
                     ILVIFGYSATSFKRAVKRRLGKVHVWAEDRNSRA"
     gene            complement(14934..16058)
                     /locus_tag="PLT86_04980"
     CDS             complement(14934..16058)
                     /locus_tag="PLT86_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35998.1"
                     /translation="MKVFANTAKRFTDEHSGIEFFLLTPDAVESIPLSYDWPAFSPDN
                     EWVIIRCQFPRESGKPSGFYRIRTDGTEIAHLSEGGIHPRLTPDGRTLFVLHPDSPVL
                     HALDLASGRDDEVCSLEKLLPDGWVFVQMRLSPASGHLFVMLRQPDIMPLRVDLRTGD
                     AVRLDDFDGMVWACAAEKPRVIVVRQRRAERGRRYTYMDYRKLELEPGDRSLWSVDVD
                     GGDEELVCVDYFSHATIHGRTTQVQGCGKWGDRSITICDAEKEPRKVCQGPYFWHSGA
                     SFDAQWIAADTNWPNYGIQLVHVPTGNFRYLCDSGSSLADGLKHPHPGLSQDGRWATF
                     RSDRSGNTQAYLVRIPDEFRQSVIAGETGDYAPVWMPQSS"
     gene            complement(16092..18098)
                     /locus_tag="PLT86_04985"
     CDS             complement(16092..18098)
                     /locus_tag="PLT86_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35999.1"
                     /translation="MVEDLSGTLTLETSDSRRPITVRSVVGGTALIALLAFITPYSEY
                     RVHSMEFFQGQIPVGALASLVVVLLPVNWFLGRFKPAWRISLPELVFMFIMAFAGIMV
                     YHIGMMGLFLSMVSSPDYFAAPENRYAEFILPYLPPAAIPSNAHNEMAYFYTGLPPGQ
                     KIPWNVWLVPLFWWWSFFAAFLLVCCALTAILRKQWFDHEKVTFPHAEVTLALVKGSG
                     EKGGLPEIMRSRIFWYGALIPSAFIVWNIISYFTPLWPRIRFSQVETGIAIQYFDSFH
                     VKPDFFTIGFAYFVDTNILFSLWFFRYLVMVQDAAYRRVGVVAATRNDPWTTANALIG
                     WECFGGLVLWTLWGLWMGRHHFRAVWQRVWDSGAGADDSNELLSYRTAVFSLAGGLLF
                     MLVWLMYLGMAWYVATIFLAILMVLIIGITRVVAESGMPIVGAPITAQAFTMRTIGDA
                     NLSPQSFVGLSLSLAAFRMIEGYPMPMVMHSARLGDIVRGRRRALFVAIFAGSIIAMI
                     LMSTTTIRLAYDGGAFNFGAHHAFHQMWEAYDHMVARIKDPWPREPSLLLHFAGGASF
                     VASLLFARYRFNWSGLHPVGFFTAMTFYHSVTTLSFLITWLIKLIINKIGGMALYNRW
                     KPFFVGLICGQVAGAVLSFVVDAIFFMGEGHDIYTMAAFGGQGW"
     gene            complement(18192..>18966)
                     /locus_tag="PLT86_04990"
     CDS             complement(18192..>18966)
                     /locus_tag="PLT86_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36000.1"
                     /translation="VAWLLTWEVAEGMYDHSYDRTWMAPYIGMPIEAVWDTVERYPKR
                     FVAGYAPDPKSPMAIEKLRTAVRHGVRVSGEHKFRTLFDDPDCIRFYRVCGELGLPVT
                     LHIDVPYLPPNDATKPVQWWYAGDLSNLERALRQCPDTVFLGHAPGFWREVSGDADSD
                     PNAYPKGPVKPGGRLRPLFDKYPNLYADLSAGSALTAISRDPEWGRKFLLDYHTRLLF
                     ARDYFDSALMDFLRSLNLPDEPFNAILSGNALRLVPLAV"
BASE COUNT         3870 a         5716 c         5689 g         3691 t
ORIGIN      
        1 caccgatttc aaacgaaccg gggacccaga cgacgtccac cttgtcgagg tctccgccat
       61 gccgctttat cgcgtccagg catcccgtga gcaggtgttc gctgatgaac gcgttaaagc
      121 ggcttacgac aatcccgaag cgatgtcctt cggcgctcag ttggccttcg tatgtcatgg
      181 ttccgtcctt ctcagaaggg gaacaactct ccgttgattc atcagaagat cccagtgata
      241 tgggaggcat ttcctcgttg gatgggttgt cgtcactcat attccagcag gtgtcctaat
      301 ttagtgcgct tggtgtggag ataccgctca ttatgaggat tcggcgggat cagtatcggt
      361 atcatctcaa ccacatcaag cccgtatcct tcaatacccg ccctttttgc cggattgttg
      421 gtcatcaggc gcacgcgctg aatgcccaga tcgcgcagaa tctgtgcacc gatgccataa
      481 tccctgatat ccggcggaaa cccgagaaac tggttcgcct gcacggtgtc gagaccgatt
      541 tcctgcaggc tgtacgcatg gagcttgttt tccagaccga taccgcgtcc ctcctgtcgc
      601 atgtagacga ccgcgcctct gccctcggct tcaattttgc gaagcgccca cgcaagctgg
      661 gctccgcaat cgcaccgggc gctgccgaat acgtcccccg tgagacattg agaatgtact
      721 cgcacgagga ccgggtcctc cgtacgaaca tcacctttca caagagcgat atggaattcc
      781 tggtcgagca ggctttcgta aaggataagc aaaaagtcgc cgtactccgt cggcatctgc
      841 acttcttcaa cgcggcggat aaggcattcg cttcggcgcc ggtgttcgat caggtctttg
      901 atcgtaacga ttctcaggcc catgcgtttt gccatcagga caagttctgg aacgcgcgcc
      961 attgtgccgt cgtcattcag gatttcacag atcaccccgg ccggcgtgtg cccggcgagg
     1021 cgcgcgagat caacggcggc ctcggtatgc cccgcgcggc gcaagacacc cccttttgcc
     1081 gccgcaaggg ggaacacatg ccccggcttg gcgaaatcct gcggtttggc ggcgggatcc
     1141 acgaacttgc gaatcgttgt cgcgcggtca cccgccgata ttccggttgt tgtaccgtgg
     1201 accgcatcaa tgctgacggt gaatgcagtc ccgtgcgccg cggtgttgtt gggagacggg
     1261 accatgagtt cgagaccgag ctgttgcaga cgggattgct ccgcggcgag gcacaccagc
     1321 ccccgggcgt gggtgaccat gaaattgatg gcatccggtg ttgccatctg cgccgacatt
     1381 acaaggtcgc cttcgttttc gcgatcctcg tcgtccacta caattaccat gcggccggcg
     1441 cgcaattcgt ccagcgcttc gccaatcgtg gcaaaaacgc ccgcttcatc cactgctatc
     1501 atgtgtccat aaaccccgct tcccgaagaa gcgctccgat gttcctgtcg ctcgctttgc
     1561 ctcccggagt cgcgctttca agcaggcggg agacgtattt cgcaagaaca tcggtttcga
     1621 ggtgcatttc gtcgcctgtc ttcctgccgc ccagtgtcgt gtgttcaaga gtgaacggga
     1681 ttacggcgac tccgaaggac gaatcgttca ccgacgtgac cgtcaggctc accccgtcga
     1741 gcgccacgga tcctttcagt gcgacctgag ggagaagctg ggatggcagc gagcaccgga
     1801 tttcgatggt atccccgagg ttcttccgct caaggagtcg gactactcca tccacatgcc
     1861 ccagaaccca atgcccgccc agccgagccc cgactttgag cgaccgttcg atgttcaccc
     1921 gccttccagg ccgccatgaa ccgatggtgg tgagacgaag cgtttccgca acggaatcga
     1981 acgtgaacgt atccgccgtc cgatccgtga cagtctggca tacaccttcc accgcgatgc
     2041 tgtcaccgag ggcgacgtcg tccatcactt ccgggcaggc gacctccgtg tgctgaacgc
     2101 cgccggaacg gacgacgcgc gtgacggttc cgactgcttc gacgattccg gtgaacatca
     2161 gggtcccgct ccgcaaaggc cggttatgca gaggtcattc ccgaaccgct cgacctcgac
     2221 ttcacgcagg cgtatggcct gatcgagcgt ttcgattccc aggtcaccta ccgccggtat
     2281 gccctcgcca aggatgatcg gggcgatgaa taccgtcagg cggttcgccg cgccggcttt
     2341 caggaagctc gtcagcgtgc gcgagccccc ttccacaaat acactctgga accgcaatgc
     2401 cgtcagagct tcaagaacag cttcaatctc gagcacgtcg ccatgtgacc tgaccggcac
     2461 cgcctgaaca tcgtttcttt cccatgcggc gatgcgttgc ggatcggccg actctgcaca
     2521 caggatcacg gtgtttccgg gctccacaag ccgggcattt ggcgaaaggc gcagacccgg
     2581 gtccagaacc acgtggtagg gctgatggcc ttccaccatc cgcaccgtga gccggggatc
     2641 gtcggccagc gcggttccca cgccgatcag caccgcatca tgccaggatc gcaaacggtg
     2701 agcgtgcgtg cgtgcttctt cacctgtaat ccactgagaa ttgccggtag acgtggccac
     2761 gcgtccatca aggctctgcg cccatttgag cgtcaaccac ggtgttccgg ttgttttcca
     2821 gtgcaggaag tgttcgttca gtctgcgagc ctcacgctcg cgcaggccca cctgaacgtc
     2881 gataccggcc ttcctcaaca gctcaatgcc acgcccgttc acgcgctcgt ccgggtcaat
     2941 gtgagaacag acaagacgtt ctatccccgc ggcgataatc cgatcggtgc atggtggggt
     3001 gcggccgtga tgagagcacg gttcgagatt cacatacatc gttgcacctc gggcggcttc
     3061 acccgcctca tccagggcaa caacttccgc gtgtgcgaga cccgcctgcc gatgataccc
     3121 ccggccgatg acggttccgt tcttgaccac gaccgcaccg accatcggat tcggggacgt
     3181 gtatcctctc cccttcgcgg ccaacgcgag ggcctcgtcc atgaaacggg cttcctgacg
     3241 ctcccgcaga cgtacgccgg aagagccggc tggcctggtg gaccgggacg aattcatcat
     3301 aagtgcggat tccgtacggc gaactcgctc aacgagcaac cgtatatttt acaggtcaaa
     3361 tgacgtgaaa accagttttg cgcacagatt gggggggcaa tacggcgctg aaggacatca
     3421 caggggggtg taccggtggc ggaagcacga ataccgccac aatcgtgttc ccctcccatc
     3481 cggactatta ccgtcggcac cggaatcgaa ccggttcgga ctgccgtcag cacgaatact
     3541 gctcgacagc tttgcgggct ttcaccgccg gtcaggaatt gcaccctgcc ccggagaacc
     3601 tgctacaacg tggtacaaaa cgtaccgact gcatgtgtga gcgtcaacgt acgcatgatt
     3661 gaagccgggt ctgatctggt gtggctttgt ggaatgagcc gggagctgga aacggccttc
     3721 gccggccgca aggttactct gtaccgccac cggacgaggc cggcgtgcct gcttgaggca
     3781 gggggcgcca gaatatgcgt tgccgatctc acccccggtt cgagccagat tcaaaatgat
     3841 tacccggttg tgcgggaaga atggcggcag tggggcacca gaagaactga cggcgctgag
     3901 gtggaacacg tccgcgtccg tgacggggac agagtgatct caattcacct cgtgtggaga
     3961 aaccgtatcg gagatgaaga acgaaccgaa ctgatctggg aacttggcgg attgcgaacc
     4021 aacgcgattc tcgtttcgct ggacggaacg gtactggatg tactgcgtcc cgtgagcgag
     4081 cgggtaaacc gagtccggac ggtgttgccg ggaagaccct atgtgccccc gcccaccgga
     4141 gtgcgacagc gtctggatga accctggacg tgggatgcgg gagatggcgc ggacacagga
     4201 ggcaacgggc ccacggtgca cgaattcctc gccagagggt tcctcgcttc aagcgggttt
     4261 ctcgcagacg aacttctggc gcgttgcggc gttgatggcg ccctgccggt ggcgcaatgc
     4321 ggccgtgaaa cgcgcgtgcg catcgagtcg gagttccgca accttcttgc ggatccgtcg
     4381 ccgcatcttc ttcttgacga gagtggcgaa ccggcgggct attgcgggta ccgcccgacg
     4441 cacgtggcac ccgagcaggt tgctcccgcc ggttctttct gcgaggcggc cacacgaaca
     4501 tggcgcgcac gccgggagtc gcaaccgggc agcgacgcga ttctcgacca gcttgccacc
     4561 tggcgtggag cactcagaaa gctactccgc cagagggaca atctccgcgg agacgtcgtc
     4621 cgcgcggaac aggcaggcga gttgcgcaga cttgctgacc tgatcatggc caatctgagt
     4681 gacattccag aaggcaccga agtagtcacg ctcacggatt ggttccagga ttccggcgcc
     4741 acggttcaag tcaggctgga tccgacgcgc aatgccgcct cgcaggcgga gcagttgtac
     4801 cgccgggcga aacggctcca gaacggtctt ccgcttatta ctgctcggtt gagaaaaacc
     4861 gaactcgcaa taaacagcct ggaacaacaa attcgggaag ccgaagatgc ggccggcggc
     4921 tcgaatgacc agcgtgtgcg gagatatcag atgcaaggtg tgtctgacgg aggagggaga
     4981 aagcagccct ttcaggggat tcggccgcgc aggtaccgca cgcgggacgg tgggtggctg
     5041 gttctggtgg gcagaaatga cgacgaaaac gatgtgctct ccctgaaagt cgccgcgcca
     5101 gatgacctgt ggttccacgc gcagggatgc cccggatccc acgtggtact gaaaaaagaa
     5161 gggcgcaaag aacaaccggg ccgacaagct attgccgagg cggcggctgt tgccgcatat
     5221 tggagcaagg cccgcggctc atcgaaagtg ggggtgagct acacgctggg aaagtatgtc
     5281 acgaagccgc ggggctcacc gcccgggacg gtcacgatcc ggcatgagaa actcataacc
     5341 gttccgccgg ccttgctgcc gctggcggac gaggcgcaag aagccaccca atgacggccc
     5401 gcagaatcgt gttcgccctt tttcgcgggg taggcgtcct tgcagcgctg ctggttatcg
     5461 cggcgacgct cgggtacctc tttgtgacaa gtcctgttgg cacccgctgg gcggcacaca
     5521 cgatcaagtc gcaggcgcgt tcactcgccg gtgttgaggc cgacatcggc ggtctggaca
     5581 ccgacatctt cacctacgtc aatctccggc gtgttcatgt cgcgttgccg gattccggac
     5641 cgtccctcca gttcgagcgg ctccagttac gcatggccct ctggcgggtc gtgacaggca
     5701 gggtgaacgg gattcggcgc attgcggcgg acagccttgt ggtgacggtg ccactcaagc
     5761 cggacacggc gaagtttgtc tggtccgtcg tcccctacct cccgtatgtc cccgaagatg
     5821 cggacatccg cgggttccga cttcgccttc tcagcgcaac cgacacgatg agtttcgcgc
     5881 tgaaagcggt ccagctacgc tcgattcccg accagcagga ttcctcgctc tacgcggaca
     5941 tcaaccttca tggcgaagtc acgctcaaac ctctcgggac actgcctttt gcgggtaaag
     6001 cgaatctctt cttccggggg gcatctctcc ggatcggcaa tctggttgtc agtggtcccg
     6061 ggttcctcgc gaagggacac gggtatctgg acgaccttgt gaacggcccc tatcaaaccc
     6121 acggttcgtt cgttgtcgac cttgcgcggc ttccgagtga ggcctccggc gtggaaatgt
     6181 ccggcacggt gaacgcggaa ggcgatatcg ggtgggatgg ccacccccgc atcaatatga
     6241 aactcgagtc tgcgaccatt tcgactccct gggcagcact ggcgaacacc acgctgttcc
     6301 tcaacacctc ggagacggac agcacgcttt cgctcagagg cgcaacttcc gtcgccgagg
     6361 gcaacgtcgg gctttccgcg acgatggttg ttccgccagc cagcggaccg ctcacggtgg
     6421 attttgccgc ggttgacgtt gatctcgatt cggtggcccg catccctgcg gtgaaggaag
     6481 ctcttgccgg gaccgaactt cggggagaag ccagcgtacg ctggcagtat tccgcacacg
     6541 cttcgatcgc cggaggattc aagctcagcc agcaggtaag ccgcatagag ctatccacaa
     6601 agggcttcca gtacgggaca atgccgatag gtacgacctc cctcgtctgg aaccagaccg
     6661 gccccgacgt ggaaggagtg ttccacggcg caggtacgtc ggttcacgtg accggcgtcg
     6721 cgaaaaacct cgacaatgtg gcactgaacg ccaatctgcg gacaccgcac ctggattccc
     6781 tgcttcaggt tttcggcgtt gacagtctgg acggtgatct tgacgttcag acaacgatca
     6841 cgggtgccct ccgcgcacct accgtcggca tcgttgccac gctgcgaaac cccgtgaacc
     6901 cctggatgcg gttacccgac ctccaactga acgggcggat ggcgcgcggg gataccgttt
     6961 cggccacatt gacaagcgta gacagcgtcc tcagggcgga tgccgttatt gtgaacaaat
     7021 tcatgcgctt cgcgtccctg agagcatcca ttggtccgct tcccttcgag caattgcccg
     7081 gcgggttcgc gaaggaacag gacatcaccg gctccctgag cgcggatatc gtcggggccg
     7141 gcctgctgac gaacccttac ctccggggct cgatacgcac cactcccgtc gcatggcacg
     7201 gccagcagat cggaacgata cggggggatt tgaactggtt cggcaactcg ctcactctgg
     7261 acgtgcgaaa tggcaacgac acgttttccg cacaggggag agtggcggcg gactccggtg
     7321 caacgtcgca gatagacgtc caatgggaaa atttcgatct tggagggatc ctttcggcgc
     7381 tgaccgacca gcccgcaggc aaaatctcgg gcatgaccga cggtacgatt tctgcacggt
     7441 ggacgcggct tgttgtcaac agcctcgtca ttcaggcacg gctggatcga gccagtctca
     7501 ggctgggtca ggcaacgtac gagcttgcgg atcccccgtc gcgtttttcc atggaaaagg
     7561 gcgttctgga ccttacaccg actctgtttc gaggctccaa cgaccagcgt ctccatttac
     7621 aggggagcat ctcgcgaagc ggtgagatga gtttccgcgc cgcgctcgat agcttcgatg
     7681 tcggcaatgc gatcagcacg ttgaccgacg ggaaactggc aatcagcggc aaaatggtgc
     7741 tccgtgcgca tcttggcgga actctcgagg agcctgttgc gaacgggaga gtcaccttcc
     7801 gaggtatcag gtggaagacc gtggagattg actcggcagg cgcactcgcc acctacagct
     7861 ccaaccagtt gagcgtgccg gagttctcat tccgcttccc gttcggcgaa gccagggggt
     7921 cattccgtgc gacggcgagt gcgctcgggc tggcagacac ggccacaggc ccgccagact
     7981 tcgcgctcag tctcgtgatg aaagatgccg gcgcggtagt tccaaactgg gagggacttc
     8041 ggagcggaac ggtaagtatt tcaggcgaac tggcccttcg aggacgggat ctggccgata
     8101 tctcgtcgta ccgcggttcg ttccggctgg attccctggc cgccgtagcg ccgttcaacc
     8161 gtctggtgcg caccaaacgg ccggccaccg tggcaatcgg agagagccgg taccttgcat
     8221 cgccgctgga cctggcaatg tcctaccgcg gggcggagac gggcgaaata tcaatcagcc
     8281 agccaccggg ggtggcccgg cccccgctgg acgtcagtat caaccgggta tcgctggagg
     8341 atttccgcca tacaatcctt ccccttctca atgcactcga tgcgcagacg tccgtaccgg
     8401 aagacctttc cggcgagttc tccgggcacg cccggtggga catgaacctg agttctcctc
     8461 gcgtggacat gagtttcttg atacgcgagc cgacggcgta ctccctgaga ggtgattcgc
     8521 tcgattgcgt tgccgcggtg agggattccg gagtgtgggt tgaatcggca aatttctaca
     8581 ctgccggcga cacgctcaac gtgaccggat ttgtggacct ttcggcggac acactcctct
     8641 tccacgcgta cacggccaac ctcgaactgg ttcccgtgct taccgacctg ttgccaccgc
     8701 tgcggacgta ccagccacca ccgccgcttc cgacaactgc accgttcccc ggattccccc
     8761 aacaaattcg agcattgccc gcggaagcgc gcggagcaac ggtgaacgct cctctgacaa
     8821 cgtttgaccg gctcgttcgg gcgcggagag gagccatgaa gcacccgtat gcggcaacgt
     8881 tgccgatggc tgcggagttc gagcttcgag ggactctgga atttcccctt ctgtttggca
     8941 atgcgcagct ttttggtgcg tatctggaga ttgaggcgct ccaggaacca atatggctgc
     9001 ccgatacgtt gagcatacgc gtcaccggaa aacgggctgt agtgccgccc gtacgggtgt
     9061 ttgtcggggc acaacagcgg gagacacaaa cccctccggc catttccctg gaacgggccg
     9121 agtattcgct cagccgcggc gaattcatcc ttgaggcgaa aattgaaagg gcaacattca
     9181 gcctggtaag tacagaaccg gtttttcttc ctcctcgctg gcgcctgttc aagggggtac
     9241 tggagcccct tctgcgcgcg tattacaccg aacccatagg cgtattcacc gccagcgcga
     9301 ctggaagctg gcggggcaca atggagcact ccaatctgaa cgccgatgtg gtgctgtaca
     9361 gctcaacgct ccagtacccg atcgcggatc ctcaggcgat tttcaagccg cgagcgccca
     9421 caagccttgg gccgttgctg gacaacacgg aactcaacat ctcgctctcc atggaggatt
     9481 cgcttgtcat caacaacaat ctgagcaaac acgcgactct gtgggcgcaa atgaacctta
     9541 cgggcaggca ttacagcccg aaccttcagg gcaacgtaca gatttccccc gggtcggtgt
     9601 tccgctatct cggccgcgac ttcgtcattc aggaggcaac ggtcaatttc ccggatccga
     9661 cgcgttttgc gcctgaaatc gaggtgaccg cacgcgcgaa ggtggtgaac accaaggaca
     9721 acaatgcgcc gtcgtatgac gtaacgcttc acgcctcagg tgtcgtgccg aatctcgtga
     9781 aaacggaaat gacggcggaa gagctcccaa gcggcgaagt aatcgtaaac cagctcgaga
     9841 tcatgcagat tctcatttac ggtcagcgca gccctgatta cctcgcaatc gatgcccagg
     9901 gacgactcaa cacaatgctg aaagagcggg ggtacgccac cttatcctcc gcactgggaa
     9961 gtgtgttgcc gatagatcag gtcaccgtgc gggaatctgc tgacaatggc gcgcaacagg
    10021 gcaccggcgg aatgaacgat gtcgaagtgg aagtcgggca gaacgttcgc gtgtggggcc
    10081 agaagctcac gttctcggcg gtatcaccgg tagggcagtt atccagcgtg agcgggcttg
    10141 aacgcgccga agtgcgttgg accatactcg agcgtcctcc tcgatggccc aaccttgagt
    10201 cactttcgct cacggtcggc cagaacagac tgcaacaggc acaggcgagc acggatctgt
    10261 acctgcaaaa cgaactcacc tccggtcttc agctccgcgt acgcttccgg tgacgggctg
    10321 cgtgacgcac gctgcgtaac gcacggggaa aggaacgaag tgcggaaacg ggaaacgaag
    10381 cggatagcac ggtttgccgc tttgatggcg ctggcgattc tttcgatgcc ccaggcggtc
    10441 ggtgccgcac ggtccacctt cgtgcaggag atcatcatca cctccactac cgtcccggct
    10501 cccgtaggcg acgagaaatg gcgacaggtt cctgagcggc gcaagctgaa cggcattctg
    10561 cagcacccca ctccgtcatt tcggaacctc tggctgccgg tccgattcga cgacgtactg
    10621 tttgcggcag atacgatgag cgtcctcacg tactatcgca atctcgggta tctgggggcg
    10681 tacgtcgcca caaccgcgca ggaaccggta ggtgggcgac tggagggact tcagtacgtc
    10741 aatttgctca tcgttatcgg aggtatcact ccagaggagc gttacaccct ccactccatt
    10801 gagttttcag gagtaacgag tcttgatacc gccgcgctgg caaaggagtt ccgcaaacgg
    10861 cacaacgccg gcaaattctt ctccccgtcc ggtgctgctg aaaacttcgt cgctctccgg
    10921 accgcatacg cggatgcggg gtatctggac tcgaacgcag tcaccatccg ccagatgccg
    10981 attgtggaca cgctcgcgcg cagtgtgcgc gagcggtacg aagttgacga gcgcacaccg
    11041 acgaccgtaa ctggttatgc aatcatcaac gggaacgcgc ctctcccgtt gatcacagac
    11101 agcgcggtcg tggttcaggc gctcaaagac gcgaagcttg tccccggatc cgtcttcggc
    11161 cggcaacgga tgctcgacgc ggaaagcaac ctgtttgacc ttggcgtgtt ccgacgcgcc
    11221 cgcgttgcgc ccgacaccgc gttcgcccgc ggatcaccat tccagcggcg cgcgatggta
    11281 gacatcgccg aacgcgacgc gggagacgtc cgggtgcaac tgggatactc gaacatccag
    11341 cagtggcgga tcagcgagac cgcaacgtac agcaacttcc ttggagaggc gcgcctgatc
    11401 ggccaggaag ggcaactgtc gcgggacgtt caggcgctgt ctttcatgta cgggcagccg
    11461 cgtatcgggg tgccttcctt cgtgccgctg gtgggcgaaa cgagcatgcg gttgcggctc
    11521 gaccaggcgt tgtccggaag ctgggaggtg cttgccgcgg gagacacgtc acggacgttc
    11581 gcgtggaaga cctctctcag ccgtcgcttt ggccggatgg cgcgaatcgg gatttcgttc
    11641 gacctctcgc gaagcgacac cacttcccgg ttccttttca gcaggccccg aagtggtgcc
    11701 acgttgcaaa gcgcggtgag tgtaaacgcc ctctacgaca cacgcgacga gtttctcaac
    11761 ccttcgcgcg ggtttgctct caacgcccag gtgagagtcg tgaacccaag ccaccgcgtt
    11821 tcgcgaatga gtatgcgtcc cgaggcgctg gccgtatact atcgcccggt gtcgccgttc
    11881 gtaatcggcg cggtgtctct gggcgggggc ttctactacg tgggaaaaga cgacagcctg
    11941 aaccaggccg agcagttctg gaggagcagg caaatgccca ccgtacgagg gttcgttcgt
    12001 gacgatatca cggtcatccg cggcgattcc gtcggcagcc ccgcggtcgc gtatctgctc
    12061 ctcaaaacgg aggcgcgcat cgcgctctgg aagcgtctta gttcggccgt gtttgtagat
    12121 gcggggcagg cctgggtatg gattccccgc gcggtgcgca atcctaccgc cgaggaacgt
    12181 gcgtttcggg ggtggagcgc catggaagcc ggacgtttcg cgctcagcgc cggattcgga
    12241 ccaaggctga actgggggct catgttccgg ctcgatctgg tccggcagat cacgccgtcc
    12301 actcactgga cctacgaatt cgggatcaca caggccttct gatttcgcct gatctacacc
    12361 aaaaagggga gccgttcagc tcccccgggg cgtaccacac atccctttga ccatcaggca
    12421 tccagagaag ggaacccgta cagcgccgcc gcattgtcat gcatgatgcg cttcgcgatc
    12481 acgagtgcct gctcctcggt gaacactccc tcttccacgc gtttcgcaag gactttcgaa
    12541 atacctgctc gagccagctt cgcgtgagca tacgcacctt ccgcgaagtt gtagtcgccc
    12601 ccaaacccca ggattttgtt cgaagggacg gtatccagca agtcgtgcaa aagccgctgt
    12661 gccatgccgg gggcaagaat gtgcgcccac gccatgtcgg ggaacacgtt gggaaacatc
    12721 ttcgcgaacg caagaacctg atcggtgtaa ggccacccgc agtggagaaa cacgaaacgg
    12781 gcggtcggat aggacataac cagattgatc agcggttccg gtttcccgtt ggcgaacaca
    12841 ttgtcgttgc tgttctgata cccggtgtga atcttgacgg gcagcttccg ttcgaccgcg
    12901 cgctggacga tctggtgcac catgtaatcc tgcaggggct tcgtttcgtc caccccgagt
    12961 ggagatggcg gaagggccac gagcacccgt tgaagagccg cttctgccgc ggcagtggtc
    13021 gtgtgagcaa aatcaagatt ccggcggtaa gcaaggaaga tcttgacagc gaccatccct
    13081 tcgttctggc gtctgtcaaa cgctgcgtcc agtgcagtct caagatcgcc aagcgagtga
    13141 atgcgcgtgt cggtctgcgc ttcgagtttg cggatatcgg cgcggctggt cgggccgacg
    13201 aaattgtcga acactggcgc ggggaagaga gaatccagat cacaaagggt gttctgccct
    13261 gcccagacaa tgcgaattga gcgggcaacg ttcgcacggt ccctgagcac ggttttgtag
    13321 aaaccgggtc ttgcaaagtt ggttgcgcgg tccgcgagca ggtcgtaggt tccttcgttg
    13381 atctcaggca catcccacag gtcgttcgcc atgattctga atgcccgtcc gtacccggta
    13441 tcctgcacat atggccagta tttcaaaaac cgcctgcgct tctcgtcgga agatagcgtg
    13501 cctccggcga attcgcccgc ttcgtccggc gagcctgccg atttgatgtc gcattccaca
    13561 tagtggagga agtagaaaag atctacctcc tttttgacat tcaatcgcgt tgcttcgtcg
    13621 tagaaatgct cgtgcgtgtc gaacaggggg attgcgtcaa ccgcggcggc aattcgttgg
    13681 taaagctcgg acatcggttc ctgctcctgt gggcggacct tgagtcggat cggttacgaa
    13741 aaggaatgta ccagacgggg agacgagttc ccaagcagtg ccggacgggg ctcctctccc
    13801 ccggacgccg gagcacgcca tattcggttg gtggggcaag ttcgcgttcg cgtagttacc
    13861 tccactcgaa ccacgaggtg ccagatggca ttgaagacgg gaagggccga aggatcggca
    13921 acagtcacga ttgatcgcgt cctgtgtgat gcgtgcgggt gctgtgtccg cgtgtgctct
    13981 gccgatgttc tccgcatgga gaacggggcc gtcattgccg acaccacacg tcttttcggg
    14041 tgcgtcgggt gcagccattg catgctcgtc tgtcctaccg acgcgattgc cgttcacgga
    14101 cgggatacgt cgctggcgga cgtgcgcgaa cttccgccac cggagatgcg cgcggatttc
    14161 gattcactgc tggctcttct cttgtcacgg agaagcgttc ggagatttcg ttcgtcggaa
    14221 gtgggggagg aggcgatcca gaagatcgtt gaagccgtgt caactgcccc gatgggattg
    14281 ccgccatcgg acgttgagat cctcgtgctc aggggaaggc cgcgggtcca ggagtttgca
    14341 ggcgacattg tggacgcgat caagcgcgca caatggttcg tttcgccgct gatgaccacg
    14401 ctcatgcgtc ggttcatcgg gaaggaggca gccgagatgt tccgcacctt cctgcggccg
    14461 cttgcgcacg cgcttcccga aaaacgcgct cgaggtgagg attggcttct gtacgacgcg
    14521 cctctcgcca tgtacttcca cacctcgccg tactcagacc ccgcagaccc tgtcattgcc
    14581 gcaacgtacg ccatgctggc ggcggaatcc ctcgggctcg gaagctgcat gatcggtacg
    14641 gtggcgccaa ttctaaaata ctacaaaaaa gtggcggaga aatatggaat cccaccgaaa
    14701 aaccagcagg gcatcctcgt cattttcggg tattctgcca catcgttcaa acgcgcggtg
    14761 aaacgccgtc tgggaaaagt gcacgtgtgg gcggaagacc ggaacagccg cgcctgaggg
    14821 ccgctcccct tgttcgcgct tcggagattc ttcgcgtcgt tcagaatgac ggttgcgttt
    14881 cgggagaatg aaaacggccc gcgcttcccg acgtcgaggc ttcttccaac gcgtcagcta
    14941 ctctgcggca tccacaccgg cgcgtagtcg cccgtctccc cggcgatgac ggattggcgg
    15001 aactcgtcgg gtattctcac caggtacgct tgtgtgttgc cggatcgatc tgagcgaaac
    15061 gtcgcccacc ttccatcctg gctgaggccg ggatgagggt gtttgaggcc atcggcgagg
    15121 gacgagccgc tgtcgcaaag atatctgaag ttgccggtgg gaacatggac aagctggatt
    15181 ccgtagttcg gccagttggt gtcggctgcg atccactgcg cgtcgaatga cgcgcctgaa
    15241 tgccagaaat aggggccctg acacaccttg cgcggctctt tctctgcgtc gcagatcgtg
    15301 atgcttcggt caccccactt tccgcacccc tgtacctgcg tggtgcggcc atgaatagtc
    15361 gcgtgggaga agtagtccac gcacacgagt tcctcatccc ccccgtccac gtccaccgac
    15421 cagagggaac ggtcgcccgg ttccaattcc agtttcctgt aatccatgta ggtgtaccgc
    15481 cgcccgcgct ccgcacgccg ctgccgtacc acgatcacgc gcggcttctc ggcggcgcac
    15541 gcccagacca tgccgtcgaa atcgtcaagt cggaccgcgt cacccgttct caggtcaacg
    15601 cgcagaggca tgatgtccgg ctgccgcaac atcacaaaca ggtgacctga ggcgggagac
    15661 agccgcatct gcacgaatac ccagccgtca gggagcaact tctcgaggct gcatacctcg
    15721 tcatcccgcc ccgaagcaag gtcgagtgcg tggagcaccg ggctgtccgg atggagaacg
    15781 aaaagagttc gaccgtcggg agtcaggcgc gggtgaatcc ctccttcgga gagatgggcg
    15841 atttcggttc cgtcggtacg gatccggtag aaccccgatg gcttgcctga ttcccgagga
    15901 aactggcacc ggatgatgac ccattcgttg tcgggtgaaa acgcgggcca gtcgtaactg
    15961 agcggaatgc tttcgacagc gtccggagtc aggaggaaga attcaattcc cgagtgctca
    16021 tcggtgaacc gttttgcggt gttggcaaat accttcattc gcacccctgc gtcgccccac
    16081 actggttcgt ctcaccagcc ctggccgcca aatgcagcca tcgtgtagat gtcgtgacct
    16141 tcgcccatga agaagatcgc gtccaccacg aacgacagaa cggcaccggc cacctggcca
    16201 cagatcaatc ccacgaagaa gggcttccac cgattgtaca gcgccatccc gccaatcttg
    16261 ttgatgatga gcttaataag ccacgtgatc aggaaactga gcgtcgtgac ggaatggtag
    16321 aacgtcattg ccgtaaaaaa acccaccgga tgcaaaccgg accagttgaa ccggtagcgg
    16381 gcgaacagga gggatgcgac gaaggaagct ccgcccgcaa agtggagcag aagcgaaggt
    16441 tccctcggcc acgggtcttt gatgcgtgcc accatgtgat cgtatgcctc ccacatctgg
    16501 tggaacgcgt ggtgtgcccc gaaattgaac gcgcctccgt cgtaggcgag gcgtatagtg
    16561 gtcgtgctca tgaggatcat ggcgatgatc gagcccgcaa aaatggcgac gaagagcgcg
    16621 cgccgccgac cacgcacgat gtccccgagc cgcgcggaat gcatcaccat gggcatcgga
    16681 tacccctcga tcatacggaa cgcggcgagg gaaagggaca acccgacaaa cgactgggga
    16741 gacagattgg catcgccaat tgtgcgcatc gtgaaggcct gggcggtaat cggagctccc
    16801 acgataggca tccctgattc ggctactaca cgggtgatgc cgatgataag caccatcagg
    16861 attgcgagaa atatcgtggc gacgtaccac gccatcccga ggtacatcaa ccacaccagc
    16921 atgaacaaca acccgcccgc cagtgaaaag acagccgtcc ggtatgaaag cagctcgttt
    16981 gagtcgtctg cgcccgcgcc agaatcccag actcgctgcc acaccgcgcg aaaatggtga
    17041 cggcccatcc acaggcccca caacgtccac aggacaaggc ccccgaagca ctcccagccg
    17101 atcaacgcgt tggcggtcgt ccacgggtca ttccgcgttg ccgcgactac gccgactcgc
    17161 cggtaggcgg catcctgcac catcacgaga tagcggaaaa accagaggct gaagagaatg
    17221 ttggtatcaa cgaaatacgc gaaaccgatc gtgaaaaagt ccggtttgac gtggaagctg
    17281 tcgaaatact gaatcgcgat ccctgtttcg acttgggaaa accggattct cggccagagt
    17341 ggagtgaagt agctgatgat attccacacg ataaaggcag aggggatgag tgcgccgtac
    17401 cagaaaatcc ggctgcgcat gatttccggt aacccgccct tttctcccga accttttacc
    17461 agggcaagcg tcacttccgc gtgggggaag gtcaccttct cgtggtcgaa ccactgtttc
    17521 ctcaagatcg cggtcaatgc gcaacaaaca agcaggaacg ctgcgaaaaa cgaccaccac
    17581 cagaacaacg gaacaagcca cacattccaa ggtatcttct gccccggggg caacccggtg
    17641 tagaaatatg ccatttcgtt gtgtgcgttg ctggggatcg ccgccggcgg gaggtagggc
    17701 aggataaact cggcgtagcg gttttcaggt gcggcgaagt aatccggcga cgataccatg
    17761 ctcaggaaaa ggcccatcat accgatgtga tagaccatta ttcctgcgaa cgccatgatg
    17821 aacatgaata cgagttctgg cagcgatatc cgccaggcgg gtttgaacct cccgagaaac
    17881 cagttgaccg gtaacaacac caccacgagt gaggccagcg cccccacggg aatctggccc
    17941 tggaagaact ccatggagtg cacgcggtac tcagagtacg gagtgatgaa ggcgagcagc
    18001 gcgatgagag cagtgccacc cactaccgaa cgcaccgtga tcggacgtct ggaatcggac
    18061 gtttcaaggg taagcgttcc gctgagatcc tccaccatgc ccggctccct ccggaagggt
    18121 gttaccttca gcaggctatc atcgcgagga agggctcgcg acgatggcca acggacacgc
    18181 tccgcggtgg cttacaccgc cagcgggaca agcctcaatg cgttccccga caaaatggcg
    18241 ttgaaaggct catccggcag gttcagcgaa cgcaggaaat ccatgagcgc gctatcgaag
    18301 tagtcgcggg cgaaaagaag gcgcgtgtgg taatcaagaa ggaatttccg gccccattct
    18361 gggtctctgc tgattgccgt gagggcgctg cctgcagaaa ggtctgcata aagattgggg
    18421 tacttgtcaa acaggggacg caacctgcca cccggcttga cggggccttt cggatatgca
    18481 ttggggtccg aatcggcatc cccggacacc tctcgccaga aacccggggc atggcccaga
    18541 aagacagtat cggggcattg cctgagtgcc cgctcgaggt tggaaaggtc ccccgcatac
    18601 caccactgga caggctttgt ggcgtcattg gggggcagat acggcacgtc gatgtggagg
    18661 gtgaccggca ggccgagttc tccacatacg cggtagaacc ggatgcagtc aggatcgtcg
    18721 aacagcgtgc ggaatttgtg ctcaccggag acccgcacgc catgtctcac ggcagttctc
    18781 agcttctcga tcgccatcgg tgatttgggg tccggcgcgt accccgccac gaaccgcttc
    18841 gggtatcgtt ccaccgtatc ccagactgct tcgatgggca ttccgatgta cggggccatc
    18901 cacgtccggt cgtaactgtg gtcgtacatt ccttccgcga cttcccacgt gagcaaccag
    18961 gccaca
//
LOCUS       DAOSOO010000044        18774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_2954_length_18774_cov_6.275335, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000044 DAOSOO010000000
VERSION     DAOSOO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_18774_cov_6.275335"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLT86_04995"
     CDS             <1..300
                     /locus_tag="PLT86_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOT36001.1"
                     /translation="HTVANGHGPVDALSTALRKALQADFPSLNDVHLNDYKVRDLSSR
                     AGTAARVRVLIETADRSTGESWGTVGVSEDILEASWQALIDSLSYKLLQDSRKKA"
     gene            complement(371..709)
                     /locus_tag="PLT86_05000"
     CDS             complement(371..709)
                     /locus_tag="PLT86_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT36002.1"
                     /translation="MKLIIAFIKPFKLDQVKDALTAINIRGMSVSEVRGFGRQRGHTE
                     LFRGSEYQVDFLPKIRIDLVVRDEDVDTVVDAIVEAGRTGQVGDGKIFVLPVEDAIRI
                     RTGEAGDDIL"
     gene            complement(722..2029)
                     /locus_tag="PLT86_05005"
     CDS             complement(722..2029)
                     /locus_tag="PLT86_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOT36003.1"
                     /translation="MLSQLNTRLAERGTTLRREVIGGITTFATMAYIVVVNPAILSDA
                     LGKEWIGALVVATCVSAAVATLLMGWWADYPIALAPGMGLNAYFAYTVVLQNHIPWQV
                     ALAAVFLSGLVFILLTLVNVQEILVTAIPLGIRNGTAAGIGIFIAFIGLQKCGFVISS
                     PATMVMGGDVTSPPFLLTLGGIALTGGLTMRKVRGAFIIGICATAVAGIALGVSPMPE
                     RLFAIPDLPTGLFGQAILRLPDAFELGLVTIVVTFLFIDLFDTAGTLSGVGSAAGFVD
                     AKGRLPRAKRAFLADGVGTSFGALMGTSTVTSYIESAAGISQGARTGIANMVTAALFL
                     LAIPLFPIAAALPGYATAPALVVVGVMMCGQMRHVNWEDIAEGLPAVAVMIGIPLTFS
                     IAEGLSFAVVLHPLILALGGRWREVHWLNWLLAGLVVLRFALI"
     gene            complement(2048..4528)
                     /locus_tag="PLT86_05010"
     CDS             complement(2048..4528)
                     /locus_tag="PLT86_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT36004.1"
                     /translation="MEFVHHPGPYHVYLVPHSHIDVEWYWTYDKTRVWSRDILESAVQ
                     LLDTMPEEAFSQDQIPVLEFLAEQRRRQSGPLISQWLTSGRMEVLGGFVQPEVAEPDG
                     ECLIRQFVLGRKRAKEMFGVAQTVGWLIDTFGQVPQIPQILVGCGMDSYVFMRGVPRR
                     LGEVPTAFWCESPDGTRVLTYWMASGYTTSESTLLDSLKEEIAHSSSPMIMIGWGADV
                     TNPADAPAGKARETLIHAAAEEGLPLASVTVATPGAFFRALREQSGNLPTACEDFAPV
                     DDRFADLRGTYSNRTRHKIVNRKAEAALLAAEAWNALAVEFGSHDFTSELGLLWQRLL
                     FSQFHDTIGGSCTDQVYEKALKRCNTVMDLAEDITDRALDVLAATADTGTFRYPVLVF
                     NPSSFPRTDIAEYEMVFREEPTNFRVVQADGKEVPHAIGNPRGSKLGTSGAIATAHVT
                     WLASEVPPLGYKVFAIEPIPGRIASVCATPGTRSVENEWYRVEVDPTTGDIAQITDLP
                     TGYRCLDGAGNEIVARKEKNPNLEGWLGLTGEEERTSRLPAEVRVEVEKNDLFQRVVT
                     RRTFLGCQLVREVSLFRGIRRIHCRTRFSGFAGGDVLVSVRFPAALSREGEIVYETPF
                     AMTTRDRSYHCAQKVVDIHDGSRGLAIINMGTAGHFPVGNALDLILFRSLRDFPDYYA
                     PLAAEHGDHSFEYAVIPHAGDWREGNVVAEGHAVNAPFLVHYAGRRKGAHSESYSLLS
                     VTPENVRISAVKRAETGAFVVVRVWETTGNPTEAKLVFNMPFSAAWSATMEEQLVAPL
                     QHEGNGITIPLRGWQISTVLIDTQPRSG"
     gene            4822..5946
                     /locus_tag="PLT86_05015"
     CDS             4822..5946
                     /locus_tag="PLT86_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36005.1"
                     /translation="MNAALEPQSLSLAFLSDRELVSSMRNGSDDAWRIILHRYTPMLL
                     AAARTYARSCASRDRPGALADIASELYLFMAERVRGSLLRFYRGECEVRTWIYRIIGD
                     RRQIIKSFMVRADETRWRADTRFPKLMQDRPRIEQEVFRRLVWGKDALWIAQDLGMTE
                     AKAHELCETILEELANKSPRVYQRIMANRKALLRPLPIEAPQWRDDKERAALELRDES
                     PLPDETMENAALSRLLSRLRDVVAGLMKSLPEEDVRLLILVFDAGWSLTEIAERAPLV
                     GLPGLSARHQVDYRINRTLGHLAQHLCEHYGRWRDDTLPTPSAAEVTAALKGFLRETG
                     VAGFAGTGKSGEGNGFAHVFQPALEGNPPFPISGTKTQGP"
     gene            complement(5883..6587)
                     /locus_tag="PLT86_05020"
     CDS             complement(5883..6587)
                     /locus_tag="PLT86_05020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT36006.1"
                     /translation="MGTRYVIPHNPGWSRLEGPATVLQRNHMVDQQMCGRGIRDARVV
                     EAMRVLPRHVFVPPERRSDAYDDHPLPIGCGQTISQPYIVALMLELLETTGKERVLEI
                     GTGSGYQTGLLALLCHHVYTVERHQELSDSARQILSDLGFRNITFHCGDGSLGWEDYA
                     PFDRIIVSAATPSLSTQLVNQLDEGGVLVAPVGDRWEQSLKAVHKRSGKTKSTDHGPC
                     VFVPLIGNGGFPSNAG"
     gene            complement(6569..7114)
                     /locus_tag="PLT86_05025"
     CDS             complement(6569..7114)
                     /locus_tag="PLT86_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOT36007.1"
                     /translation="MTPREYAHAQYRLTDITQAPVDLVPIIRHHQLRIVEVEVLDRAD
                     SRLQLSNTAGFIRIRRDIDHTRKRWEIARQIGLFVMLNEGERPRVRDTSVFASELLMP
                     AELVVRLYRNRREWLKNAMLVGMSATKRDMIRGLSWQFEVDDRAIAIRLDELHLGPGA
                     EPNKRIWSSSPGVIPRWEPDT"
     gene            complement(7137..8309)
                     /locus_tag="PLT86_05030"
     CDS             complement(7137..8309)
                     /locus_tag="PLT86_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOT36008.1"
                     /translation="MRSALASLPERVLLGPGPSNVHPRVMRAISAPVIGHLDPAFISV
                     MEELKDLLRKVFCTRNEFTIPFSGTGSAGMEAALANMIEPADSVIVCINGVFGTRMKE
                     IAERCGANVVTVEAPWGSAIDAGDVEKAMKVTPGVKLVALVHAETSTGVLQPVDDIWR
                     LAHEHGALVLADTVTSLGGAPVLTDDWGLDIVYSGTQKCLSCPPGLAPVTFNERALNV
                     IRGRKTKVQSWYLDATMLMRYWGEERVYHHTAPISMNYALCEALHLVLEEGLEARWKR
                     HVWNHRALVAGIEAMGLSMAVNEAIRAPMLNAVVVPDGIDEKRVRRSMLDGMNVEIGA
                     GLGPLAGKIWRIGLMGYSSRPENVLTCLSALNAALREQGRRCPDGLNAAIDAFQTD"
     gene            complement(8321..9553)
                     /locus_tag="PLT86_05035"
     CDS             complement(8321..9553)
                     /locus_tag="PLT86_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT36009.1"
                     /translation="MLIRLLCGTGVFSFFLLCTTVIASPQNPDGDLSNRLEVQQAELA
                     RIEQELAATNAEMRMAKARERALASQVVAASNRAGLTKHAINRLTEAETALVSDMNSA
                     QIRTQQTREKIERRRESISLRVRTLYIHGRREPFQRALMASSLAHWLAAREYLTAAAR
                     RDANEIRSLKRDQVRLDSLTALYSRQKMLLDTLITTRERQREELAGAEMEARRLLRAM
                     RAKRRTLEQSAEALESQRAESRQRIAEFLSSQTGASPTRMPLLEQGPGLREETGFVNL
                     KGKLPWPVEGRVTSRFGRRRDAATQTWTRSRGIDIRVPKGTEVTAVGAGKIVMVDWFR
                     GYGSFVIVSHGQNYYTLYAHLDEIHVQKNEHVQQGQVIGLSGDSGTLGEEKLHFELLG
                     GPQALNPLDWLQPQRTGL"
     gene            complement(9547..10455)
                     /locus_tag="PLT86_05040"
     CDS             complement(9547..10455)
                     /locus_tag="PLT86_05040"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT36010.1"
                     /translation="MFWSTSTTEAWRAVSSAKRATISSVLIMAVSLAILGILGLGALA
                     FHNEAQTAKRWITAEVFLKDGLEPEAIQLVRRRIVAIEGVSNARLITKAEALVRFKRF
                     FGSELVDVLETNPLPQSYLLTLSDEGRTPEGLKAIARKAGSFPEVESVDADVEWLTIL
                     ERISFTVNVVLLLFLGIVGFAISVVISRTIGLGIASRAEVVTLQRLLGASEWFVRRPF
                     VILGVAQGAVGGILAALIVLACSRFAYAIPLVGRSFGGTNAHVAAWSLVGVGVVLGLA
                     GSISTLRSSLPRDPWESEQVTRNSLC"
     gene            complement(10613..12139)
                     /locus_tag="PLT86_05045"
     CDS             complement(10613..12139)
                     /locus_tag="PLT86_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT36011.1"
                     /translation="MKSRAVAECVFVAILNAFAFEAIPASQEQYVRLSLQECRIRALQ
                     NNLSFRVKRLEPLISEARLKAALAPFDPAITASFSGSRTQQPNSLVFDPSTGTYISQS
                     VPAQNSLGTRLGISKAFSFGTNASINLNNSWSKGSGSSNYGSNVSLSVSQPLLRGFGR
                     DANEFGIRIARANRETSMKQIVADASAAVQKVESAYWDLVSARENLRVAQMSHQYAQQ
                     LLERSRARAETGAQALRDVIQAEAGVATAYQGIISAEARVRDAEDTLRRLTNIAAMED
                     GWSFGIVPTDTTHIDSGTPSFDSLFAAALENNPAVFQAAQAVETSKFTLKYRYNGLLP
                     TLDASTEVVLRDGDRTAARSMDDLPTAAYPSWTLGLRFSVPLGNRQSEASYREAIIAV
                     RTAELELQDARQAVQADVRSALRRVETSRQQIAAAQETKRLRESTLENEQERLRLGLS
                     TNYDVLQVERDVADARRAYVEARVEHEKALLALDVATGLLLRKRNIGVEMDGSELTSR
                     "
     gene            12438..13187
                     /locus_tag="PLT86_05050"
     CDS             12438..13187
                     /locus_tag="PLT86_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36012.1"
                     /translation="MLKITKKQRERWPRELILSEKSEELEEHLTLLNAEDRAAVERVS
                     REVDSKIVEAARESVKELKMLEEGRCPACSQKTRRFLFSTICEHCGWSGYSRPLRAKV
                     VIHLRDGSALICEDVYHTPDELMCVTQDVVRYRVPRNVVAYVEFDHAEEDIRQRREQL
                     AREEQGQCSWCMKPVVRTNEDTRVTFAAFGIYQERFFFCSIHCQTAFQKQYPTRIHRD
                     CYHRDCSKCEECQKRFDDTSYETFLEEEFVH"
     gene            complement(13222..14190)
                     /locus_tag="PLT86_05055"
     CDS             complement(13222..14190)
                     /locus_tag="PLT86_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOT36013.1"
                     /translation="MGNQPDRHHLHAPLEIGGTVFHAAEIAQMPTFLSYAGNARPWLW
                     AAYFPFLLAYSDSPARAVMLGEHGGSLFVLLHRAVRGHGHIDFLLPPLGSGYEGALEN
                     LTGSLARLNGPIETRFLWADLPGAAYGSRRGWVVTPYEHEYVYQRRALIELRGNEFRA
                     LRKRIHRCEREIQPEFRAFVESDVPECEALLREWQDMHRQDPVPVFDFGYTLSAIRSA
                     ARFRPPHLVGVVAVVGGKVRAFAFGGMIWEGLGQFFVLKSDPACKGLAEATRLVLAER
                     MEGCDLVNDAGDLSKPGLAQHKRLFRPVSFVPTFKMACRSALSPDS"
     gene            complement(14202..14558)
                     /gene="speD"
                     /locus_tag="PLT86_05060"
     CDS             complement(14202..14558)
                     /gene="speD"
                     /locus_tag="PLT86_05060"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOT36014.1"
                     /translation="MTCSPPVHLLIDFVVDDPVLLNTPDPSMSFLRESVLAAGATILS
                     EHTHQFSPVGFSGILILAQSHASIHTWAECRLVSIDIFACGAIDTEAAVDVLRNRFRP
                     SSERIEICRRGVGAFK"
     gene            complement(14555..15739)
                     /locus_tag="PLT86_05065"
     CDS             complement(14555..15739)
                     /locus_tag="PLT86_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HOT36015.1"
                     /translation="MALREGHEQLHAGDGGQILSEVASASHLREGREGVRTILRAIAT
                     LEPVPVRVLASKVRLPLPVTVAVIGELSARGMTERAPNGVRLTSAGASCVETICGDAG
                     HDETTGLSAVLDDLAALRPQADVTLDQAKATVGTLVRRVNYLLKNDSLWGRRILFLGD
                     DDFVSVAVARWVHQCAGGEPAGPTPTLTVVDIDGRVCDGIKKAAPSVQTFVHDLRKPL
                     PDSLQHRFDVVFTDPPYTPGGAELFLCRAAEAVEHEGAHCFFSMGHLDPGGMRQVQAA
                     ISGIGWVIVEWIPAFNEYEGSSVLANVSLMAHLIATPEIAPTLRETYEGPLYTADVRP
                     VIRVYRCLGCSAEWEVGQGKRWITVERLKEDRCPLCRSDRFLRARQYSRAEFEKPGGA
                     QN"
     gene            complement(15717..16547)
                     /locus_tag="PLT86_05070"
     CDS             complement(15717..16547)
                     /locus_tag="PLT86_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36016.1"
                     /translation="MGCTIFWEAQEFSLSRQAQVRDELVALAQVAGWEFEIAPDELTG
                     AFINLDLQPGEARIIERRLRLCGVTLFPFGNDEQGIRPRLSFVFDNSNLSNPNVRNRL
                     ITIAPPSYWTYWWPRLREQYPLLMNLPDRPILCIRGDGNMRVRRTELPGFIKFVNTLR
                     AGSLPLLDIRTSDKLARKMISEPQGGQFGARGARRQEQESLADIAGGLAALFASSRFD
                     EWRAALQTEDDIEREQARANRAAALPQERERRSSRQDRPGTGTRGFSFGSTYGAPGRS
                     "
     gene            16867..18594
                     /locus_tag="PLT86_05075"
     CDS             16867..18594
                     /locus_tag="PLT86_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOT36017.1"
                     /translation="MVSDADLLDKITDYTRKQGGDTAIERLLANVVSQVREFAEARAS
                     EIKRLSDIGIALSAERNLERLLERIVDEARRFTRADAGTLYTVQINPATNLPELKFDI
                     MQNDTMNTRQGGTSGNPITLPPVPLVKDGKPNHNNVSAYVADTGETVNIPDVYIAEGF
                     DFTGPRKFDQITGYRTKSMLVAPMRNHEGEIIGVLQLLNAKVPGGERVIPFSDEYVDL
                     IKSLASQAAVAITTARLIQDLQNLFESVIRLVASAIDEKSAYTGGHITRVAGLSMKIA
                     EAINETTEGDFADVKFTPDELNELRIAAWLHDIGKITTPEYVVDKGTKLETIFDRIEL
                     VKTRFEVIRRGVIADAMEYKMRLIANGADPEALKAVDEQLAVRLEEIESDKEFVARCN
                     TPGEFLKDDAIDRLKAIAAKTFLLDGVETPYLTENELKNLSIRKGSLTEEERQIINNH
                     VSVTIKMLSQLPFPKKLSHVVEYAGAHHEKLNGAGYPNGWTAERLAIQSRILCLADIC
                     EALTAADRPYKPSMPKERAFQILGFMAKDQEIDERLLKLFLEKEVYDRYMVEYKAQKE
                     AEAAAATAK"
BASE COUNT         4004 a         5579 c         5423 g         3768 t
ORIGIN      
        1 catacggtgg cgaacggcca cggcccggtg gatgcactga gcacggctct tcggaaggcc
       61 ctgcaggccg atttcccgtc cctgaacgac gtgcacctca acgattataa agtccgcgac
      121 ctgtcaagtc gcgcaggcac ggcagcgcgg gtgcgcgttc tgatagaaac cgcggatcga
      181 tccacgggag agtcgtgggg cacggtaggc gtgtcagaag atatcctcga ggcgagctgg
      241 caggcactga tcgacagttt gagttacaag ctgctgcagg atagccgcaa gaaggcctga
      301 tgtacaacaa cgaaggggac agatttccga ttctgtcccc ttcgtgaatg ccgacagact
      361 ccccgcgcaa tcaaaggatg tcgtcacccg cttccccggt gcgtatccgg atggcatcct
      421 ctactggaag cacgaaaatc ttcccatctc ccacctgacc cgtgcggccc gcctccacaa
      481 tcgcgtccac caccgtgtcg acgtcttcgt ctcgaacgac gaggtcgata cggattttgg
      541 gaagaaagtc cacctgatac tcgctcccgc ggaacagctc cgtgtgacca cgctgccgac
      601 cgaatccgcg gacttcagaa accgacatcc cgcggatgtt gatcgcggtg agcgcatcct
      661 tgacctggtc aagtttgaac ggtttgataa acgcaataat cagcttcacg gttctgctcc
      721 ttcagatgag cgcaaagcgg agcacgacca ggccagccag aagccagttc agccagtgaa
      781 cttctcgcca ccttccgccg agagcaagga tgaggggatg cagcacaacg gcgaaagaaa
      841 gcccctccgc gatactgaaa gtaagcggta taccaatcat aacggcaact gccggcaacc
      901 cctccgcgat atcctcccag ttcacgtgtc tcatctgccc gcacatcata acgcccacaa
      961 ccaccagcgc gggcgccgtt gcgtatcctg gcaacgcggc cgctatcggg aagagcggta
     1021 ttgcaagaag gaacaacgcc gcagtaacca tgttcgcaat accggtgcgc gcgccctggg
     1081 aaatgcctgc tgcgctttcg atgtaactgg taacggtgct ggttcccata agcgcgccga
     1141 agctcgtacc cacgccatct gcgaggaaag cgcgttttgc tcgtggcagg cgcccctttg
     1201 cgtccacgaa tccggccgcg cttccaactc cgctcaaagt accggcggtg tcgaacagat
     1261 cgatgaaaag aaacgtaaca acgatggtaa cgaggcccag ttcgaacgcg tcgggtagac
     1321 gaagaatagc ctgtccgaaa agtcctgtcg gcaaatcagg aatcgcaaaa agacgttccg
     1381 gcatcggcga aacacccagg gcgattcccg caacggcggt ggcgcagatt ccgataatga
     1441 acgcgccacg cacttttcgc atcgtgagac caccggtcag cgcgattccg ccgagcgtga
     1501 ggagaaacgg aggagaggtg acatcgcccc ccatcaccat tgtggcgggt gacgagatga
     1561 caaagccgca tttctggagc ccgatgaagg caatgaagat acctatcccg gcagccgtac
     1621 cgttgcggat tccaagcgga atggcagtga caagaatctc ctgcacgttt accaaggtca
     1681 gcagaatgaa cacaagtccg cttaagaaca ccgcagccag agcgacctgc cacgggatgt
     1741 gattctggag aacgactgtg tatgcgaaat acgcgttcaa ccccatgccg ggggcaagtg
     1801 cgatggggta gtccgcccac catcccatga gcagcgtcgc aaccgcggca gaaacgcagg
     1861 tagcgacgac cagcgcgccg atccattcct tgcccagagc gtcgctgagg atcgcggggt
     1921 tcactaccac aatgtacgcc atggtggcga acgtcgttat tccgccaatc acttcccttc
     1981 gtaacgtagt gccacgctcc gcaaggcggg tattcaattg cgatagcacg ggttgtctcc
     2041 ttccgcgtca gccagaacga ggctgtgtat cgatcaggac ggtcgaaatc tgccagcctc
     2101 gcaacggaat tgttatccca tttccctcat gttgaagcgg cgcgaccagt tgctcttcca
     2161 tcgtggcgct ccatgcggca gagaagggca tattgaaaac cagtttcgcc tctgtcgggt
     2221 tgccggttgt ttcccacacg cggacgacga caaatgcacc tgtttctgcc cgcttcactg
     2281 cgcttatgcg gacgttttcc ggcgttaccg aaagcaggga gtacgactcg gaatgagccc
     2341 ctttccgcct gcctgcgtag tgaaccaaaa agggtgcgtt tactgcgtgg ccttcagcca
     2401 caacatttcc ttcacgccag tctcccgcgt gtggaatgac tgcgtattcg aacgaatggt
     2461 cgccgtgttc ggcagcaagc ggcgcatagt agtcgggaaa atcacggagg ctgcggaaaa
     2521 ggatcaggtc aagagcattt cccaccggga aatggcctgc cgtccccatg ttgattatgg
     2581 ccaatcctcg tgaaccgtcg tgtatgtcga ccaccttctg ggcgcaatgg taagaacgat
     2641 cgcgcgtggt catcgcgaag ggggtctcat acactatctc gccctcgcgg gaaagggccg
     2701 cggggaaacg aacagataca agcacatcgc cgcctgcaaa tcccgagaaa cgagtcctgc
     2761 agtgaattcg ccggatcccc cggaaaagcg agacctctcg aacgagttga caaccgagga
     2821 atgtgcgtcg cgtaaccacc cgctgaaaga ggtcattctt ctcgacttct actcgaactt
     2881 ccgccggaag gcggctcgta cgctcttcct ccccggtgag gcccagccat ccttccagat
     2941 tcgggttctt ttctttcctc gcgacaatct cgttacctgc cccatcgaga cagcgatagc
     3001 ctgtcggaag gtccgttatc tgggcgatgt ctccggttgt cgggtctacc tccacccggt
     3061 accactcgtt ttccacggaa cgggtcccag gtgtcgcgca gactgatgca atgcgccccg
     3121 gtatcggttc gatggcgaag actttgtagc ccagtggggg gacctcgctg gccaaccagg
     3181 taacgtgcgc tgtcgcgatt gcgcccgagg ttccaagctt ggagcccctc gggtttccga
     3241 tggcgtgggg cacctccttc ccgtccgcct gaacgaccct gaagtttgtt ggctcttccc
     3301 tgaataccat ttcgtactca gcgatgtctg ttcgagggaa agaggaaggg ttgaagacga
     3361 gcaccgggta ccggaaggtg cccgtgtcgg cggttgcagc cagcacatcc agcgcacgat
     3421 cggtgatgtc ttcggcgagg tccatgaccg tgttgcacct cttcaatgcc ttctcataca
     3481 cctggtcggt gcaacttccg ccgattgtgt cgtggaactg cgagaatagc agacgttgcc
     3541 agaggagccc cagctcgctc gtgaagtcat ggcttccaaa ttccaccgcg agcgcgttcc
     3601 aggcttccgc cgcgagaagt gccgcttccg ccttccgatt gacgatcttg tggcgcgtcc
     3661 gattcgagta cgttccccga aggtccgcga agcggtcgtc tactggggca aaatcctcgc
     3721 atgctgtcgg caaattaccc gattgctcgc gaagcgcccg gaagaaggca ccgggcgttg
     3781 cgacggtgac tgaggccagt ggaagcccct cttctgcggc cgcatggatc agggtctctc
     3841 gcgccttccc cgcgggcgcg tccgcggggt tggtgacgtc cgcgccccag ccgatcatga
     3901 tcatgggaga agacgagtgg gcgatctctt ccttcaggga gtccagaaga gttgattccg
     3961 acgttgtgta cccgcttgcc atccagtacg tcagaacgcg ggtcccgtcc ggcgactcgc
     4021 accagaatgc cgttggcact tctcccagcc ggcgtggcac ccctcgcatg aagacgtagg
     4081 agtccattcc gcatccgacg agaatctgcg gtatctgcgg aacctggccg aatgtgtcaa
     4141 tcaaccagcc gaccgtctgc gcgacgccga acatctcttt cgcccgtttc ctccccagca
     4201 cgaactgccg gatgagacac tcgccgtcag gctctgctac ttcgggttga acaaacccgc
     4261 caaggacctc cattctgccc gaggtgagcc actgtgaaat cagcggtcct gactggcgtc
     4321 gtcgctgttc cgcaagaaac tcaagaacag gtatctggtc ttgggaaaag gcttcttccg
     4381 gcatcgtatc gagcagttgg actgcgctct ccagaatatc gcgcgaccac acccgcgttt
     4441 tgtcgtaggt ccagtaccac tcaacgtcga tgtgcgagtg agggacgagg tatacgtggt
     4501 acggccccgg atggtgaaca aattccattt gtgcgttccg tttgcggtga agagtggaat
     4561 ggcttcaggg cgactgcttt gcggtttcac gaaataaggt accctcgcgc cttctgaagg
     4621 gaaacgccac ctgcgttgga actccccgaa cgggaaagga tgttgttacg cggagagaag
     4681 gaccgagccc gaaaaacggc atctgttccg tgtgatgttt cttacgggaa tttcgcggcc
     4741 gttcgaggcc aaaccatgcc tggtgctgca acgggcagct cgaagtgatg gcccgcaatg
     4801 cgtgaaggtc gaaaccacac catgaacgct gctcttgaac cgcaaagtct ttcactcgca
     4861 ttcctttcag accgcgagct cgtgtcctcc atgcggaacg gttcggacga tgcgtggagg
     4921 atcattctcc atcgctacac accgatgctt cttgcagcgg ccagaaccta cgcacgcagt
     4981 tgcgcctctc gcgaccgccc aggggcgctc gcggatattg catcagagct ctacctgttc
     5041 atggcggaac gggttcgtgg gagtctcctc cggttctatc gtggcgagtg cgaggtgcga
     5101 acctggatat accggataat cggcgacaga cggcagatca tcaaatcgtt catggttcgt
     5161 gccgacgaga cacggtggcg tgcagacacg cgcttcccga agctcatgca ggaccgcccc
     5221 agaatcgaac aggaggtgtt ccggaggctc gtatggggca aagacgcgct ctggatcgcg
     5281 caggacctcg gcatgacaga ggcgaaggct catgaactct gtgaaacgat tctcgaggaa
     5341 ctcgcgaaca aaagcccccg cgtgtaccaa cgaatcatgg caaaccgcaa ggcgcttcta
     5401 cgtccgctgc cgattgaagc gcctcaatgg cgggatgaca aggagcgtgc ggcgctggag
     5461 ctgcgtgacg agtctcccct gcccgacgaa accatggaaa acgcggcatt gagtcgcctc
     5521 ctttccaggc tccgggacgt ggtggcgggg ctcatgaagt ccttgccgga ggaggatgtc
     5581 cggcttctca ttctcgtgtt tgacgctggc tggtcgctga ctgaaatcgc ggaacgtgcg
     5641 ccactggtcg ggctgccggg tctgtccgcc cgccatcagg tggattaccg gatcaaccgt
     5701 acactcgggc accttgcgca acacctctgc gaacattacg gcagatggcg tgacgatacg
     5761 ttgccgactc cgagcgcggc cgaggtgacg gcggctctga aaggcttcct gagggagact
     5821 ggggttgccg ggtttgcagg caccgggaaa agcggcgaag ggaacggatt cgcacacgtg
     5881 tttcagccgg cgttggaggg gaaccctcca ttcccgataa gcggaacgaa gacgcagggg
     5941 ccatgatcgg tagacttcgt tttgccggac cgcttgtgca ctgccttcag agactgctcc
     6001 caccggtcgc caaccggggc aacgagcacg cctccttcgt ccagttggtt cacaagctgg
     6061 gttgagagcg acggagttgc ggcggagaca atgattctgt cgaacggcgc gtagtcctcc
     6121 cacccgagag acccgtcccc gcagtggaag gtgatgtttc tgaaaccgag gtccgaaaga
     6181 atttggcgag cagaatcaga gagctcctgg tgtctctcca cggtgtagac gtgatgacag
     6241 agcagcgcca gaaggcccgt ctggtacccc gatcccgttc cgatctccag aactcgttcc
     6301 ttccctgtgg tttccagtag ttccagcatg agcgcgacga tatacggttg agatatggtc
     6361 tgcccgcacc cgattggcag aggatggtca tcgtacgcgt cggaacggcg ctccggtggc
     6421 acgaacacgt gtcgtggcag tacgcgcatc gcctctacta ctcgcgcgtc ccgaataccc
     6481 cgcccgcaca tctgttggtc taccatgtga ttgcgctgaa gaaccgtggc aggaccttcg
     6541 aggcgagacc aaccggggtt gtgggggatc acgtatcggg ttcccatctc gggattactc
     6601 ccgggctact cgaccagatt cttttgttcg gctccgcacc gggtccgagg tgcaattcat
     6661 ccagacgtat ggcaattgcc ctgtcatcca cttcgaactg ccacgacagc ccacggatca
     6721 tgtcgcgttt cgttgcgctc atccccacca gcatggcgtt tttcagccac tccctgcggt
     6781 tgcggtagag acgcacgaca agctctgcgg gcatcagaag ctcagacgcg aaaaccgaag
     6841 tgtctcgaac acgtggccgt tctccttcat tcagcatcac gaagagacca atctggcggg
     6901 cgatttccca ccgcttgcgc gtgtggtcaa tatcccgccg tattctgatg aaccccgcgg
     6961 tgttcgacaa ctggagtctg ctgtctgcgc gatcgaggac ctcgacttcg acgattcgca
     7021 gttgatggtg gcggatgatt ggaacgaggt ccacaggcgc ctgcgtgatg tctgtcagac
     7081 gatactgggc atgtgcatac tcccgtggcg tcatgcgtgt ctcccaaatc gcaaaatcaa
     7141 tccgtctgga acgcgtcgat agcagcgttg agtccgtccg gacaacgtct cccctgttcg
     7201 cgaagcgctg cgttcagagc ggaaaggcag gtcaggacat tttcaggcct gctcgaatac
     7261 cccatcaacc cgattcgcca gattttgccg gcgagaggcc ccagccccgc tccgatttcc
     7321 acattcatcc cgtccaacat cgaacgacgc acccttttct cgtcgatgcc gtccggaaca
     7381 accactgcgt tgagcatcgg tgcccgaatg gcttcgttca cggccatcga aagccccatc
     7441 gcttcgattc cagcgacaag cgcgcggtgg ttccagacgt gtcgcttcca gcgcgcctca
     7501 agtccttcct ccagcacaag atgcaaagcc tcacagagcg cataattcat cgaaatcggc
     7561 gccgtgtggt ggtagacgcg ctcctcgccc cagtaacgca tcagcattgt ggcatcaaga
     7621 taccagctct gcaccttcgt ctttcttcca cgaatcacat tcagcgctcg ttcgttgaat
     7681 gtgaccggcg ccagtcccgg tgggcagctc aggcacttct gagtgccgct gtacacgata
     7741 tccagtcccc agtcgtcggt gaggactggg gctccgccga gcgacgtgac cgtgtccgcg
     7801 aggacaagcg cgccgtgttc gtgtgcaagc ctccaaatgt cgtccaccgg ttggaggact
     7861 cccgtagatg tctccgcgtg aacaagcgca acgagcttca ccccgggcgt taccttcatc
     7921 gccttttcca cgtcaccggc gtcgatggcg cttccccacg gcgcttccac cgtaacgacg
     7981 ttcgccccgc agcgctcggc gatctctttc atccgtgtgc caaacacacc gttgatgcac
     8041 acgatgacgc tgtccgccgg ttcgatcatg ttcgcaaggg ccgcttccat ccccgccgaa
     8101 cccgtgcccg aaaatggaat cgtgaattcg ttccttgtgc agaacacctt ccgaagcaga
     8161 tctttgagct cctccatcac gctgatgaac gcgggatcaa ggtggccaat caccggggcg
     8221 gaaatcgcac gcatcacgcg tggatggacg ttggaagggc cgggtcccag caaaaccctt
     8281 tcgggaagtg acgcgagagc tgaacgcatc ggtgctcctt tcagaggcca gttcgttggg
     8341 gctgaagcca gtcgagcggg ttcagtgcct gcggaccgcc gagaagctcg aagtgcagct
     8401 tttcctcacc aagcgtgccg ctgtcgccgg acaatccgat cacctggccc tgctgaacgt
     8461 gttcgttctt ttggacatgg atctcgtcaa gatgcgcgta caacgtgtag taattctggc
     8521 cgtgtgaaac gataacaaac gagccgtacc cacgaaacca gtccaccatg acaattttcc
     8581 cggctcccac ggcggttact tcggttcctt ttggaactcg gatgtcaatg cctctactcc
     8641 gcgtccacgt ctgcgtggcc gcatctctcc tgcgaccgaa acgagatgta acgcggccct
     8701 ccaccggcca cgggagcttt cctttgaggt tgacgaatcc cgtttcctcg cgcagaccgg
     8761 gaccctgttc gagcaacggc atgcgggtgg gggatgcccc cgtctgcgag gaaaggaatt
     8821 cggcgattcg ctgtctgctt tccgctcgct gactctcaag cgcttcggcg gattgctcca
     8881 acgttcgtct cttcgcgcgc attgcacgca agagcctccg tgcctccatt tctgcgccgg
     8941 ccaattcctc ccgttgccgc tcgcgcgttg tgatgagggt atccagaagc attttctggc
     9001 gggagtacaa agccgtcaac gagtccagcc gcacctggtc acgcttcaat gaacgtatct
     9061 catttgcgtc acgtcgtgcg gccgccgtga ggtattccct cgccgcaagc cagtgcgcga
     9121 gcgacgacgc catcaatgcg cgctggaaag gttcgcgtcg cccgtggata tagagagttc
     9181 tcacccgcag cgaaatcgat tccctccggc gctcgatctt ctcccgtgtt tgctgtgttc
     9241 tgatctgcgc gctgttcatg tcggaaacaa gcgcagtttc cgcttcggta agacggttga
     9301 tcgcgtgctt ggtcaatccg gcgcggttcg acgcagccac cacctgtgag gccaacgccc
     9361 gttcacgcgc tttcgccatc cgcatttctg cgttcgtggc ggccagttcc tgctcaatcc
     9421 tagccaactc ggcctgctgg acttccaaac ggttactgag atcaccatcg gggttctgtg
     9481 gcgacgcaat aacggttgtg cagaggagga agaaagagaa gacgcccgtt ccacacaaaa
     9541 gccgtatcaa cacagggaat tcctcgttac ctgctcgctt tcccaggggt cacgggggag
     9601 cgaactgcgc agggtggaga tgcttcccgc aagccccaga acgacgccaa ctccaaccag
     9661 cgaccatgcc gccacgtggg cgttcgttcc tccaaagctg cggccgacca gtggaatcgc
     9721 gtatgcgaag cgcgaacacg cgagcacaat gagggccgcg agaattccac caactgctcc
     9781 ctgtgcaacg ccgagaatta caaacgggcg ccgcacaaac cattcagaag ctccaaggag
     9841 ccgttgtagc gtcaccactt ctgcgcggct cgcaatgccg agaccaatgg ttcgtgaaat
     9901 caccacgctg atcgcgaagc ccacgattcc aagaaaaagc agcaacacga cgttgacagt
     9961 gaacgagata cgttcgagta tggttaacca ttcaacgtcg gcgtcgacgg actcaacttc
    10021 ggggaagctg ccagccttcc gggcgatggc cttgaggcct tcaggcgttc ttccttcgtc
    10081 actcagggta aggaggtagg attgagggag aggattcgtt tcgagtacat ctaccagctc
    10141 ggaaccgaaa aaccgtttga agcggaccag ggcctccgct ttcgtgataa gccgcgcatt
    10201 gcttacgccc tcgattgcaa cgatccgccg cctgacgagc tgaatcgcct ccggttcgag
    10261 gccgtctttg aggaagacct cggcggtgat ccatctcttt gcggtttgcg cttcgttgtg
    10321 gaacgcgaga gcgcccagac cgagaattcc gagtatcgcg agggaaacag ccatgatcag
    10381 cacggagctg atcgtagccc gtttcgcgga actgacggca cgccacgcct cggtggtgct
    10441 tgtgctccag aacacgttat actccctgca gacatcgccc cctcatgggc gattgcaggg
    10501 gaaaaataag gggttgctca ggccgagtcc aaggaatcgc attcgtattc ctcacgtcgc
    10561 accggcaaaa gaacacggac cgttaccaga gtcgcgcatg attcgagaag gcctatcggc
    10621 tcgtcaattc cgaaccatcc atctcaacac cgatgttccg tttgcggagg aggaggccgg
    10681 tggccacatc gagtgcgagc aacgctttct cgtgctccac gcgcgcctcc acgtacgcgc
    10741 gccgcgcgtc cgcaacgtcc cgctcaacct gcagaacatc atagttggta gacagaccca
    10801 ggcgcagcct ttcctgttcg ttctcaagcg ttgattcccg caacctcttc gtctcctgcg
    10861 cggccgcgat ctgttgccgg cttgtttcga cacgtcgtag agccgacctc acatccgcct
    10921 gcacagcctg gcgcgcgtcc tgaagctcca gttctgccgt tctcactgca atgatggctt
    10981 cacggtaact cgcttctgac tgacgattgc caagaggaac cgagaaacga agccccaacg
    11041 tccatgacgg gtatgctgcc gtgggaagat catccatgct tctggctgcc gttcggtctc
    11101 catcccggag tacaacctcc gtcgaggcgt ccagagtcgg gagaagcccg ttgtatcggt
    11161 atttcagagt gaatttgctg gtttcgaccg cctgtgccgc ctgaaacacc gcagggttgt
    11221 tttcaagagc cgcagcgaaa agtgaatcga atgacggcgt tccagaatca atgtgggtcg
    11281 tgtctgtcgg gactattcca aatgaccacc cgtcctccat cgccgcgatg tttgtcagac
    11341 gtctgagggt gtcttcggca tcccgaacgc gtgcctcggc ggatatgatt ccctggtatg
    11401 cggtggcgac accggcttcc gcctgaatta cgtctctgag tgcctgcgca ccggtttccg
    11461 cacgcgcacg ggatcgctcc agcagttgtt gggcgtactg gtgtgacatc tgtgcgacgc
    11521 gaagattctc acgtgcgctc accagatccc agtacgcgga ttccaccttt tgaacggcgg
    11581 ccgacgcatc cgccacgatc tgtttcatgc tggtttcacg attcgcccgc gcgatccgta
    11641 tgccgaactc gttggcgtcc cgcccgaaac ccctgagcaa cggttgcgaa acgctcaggg
    11701 aaacgttcga cccgtaattc gagcttcccg agcctttcga ccacgagttg ttcagattga
    11761 tactggcgtt ggtaccgaaa gaaaacgctt tggagatgcc tagccttgta cccaggctgt
    11821 tttgcgcggg taccgactgc gagatgtacg tgcccgtaga gggatcgaac acaagcgaat
    11881 tgggctgttg cgtccgtgat ccagaaaagg aggcggtgat cgccgggtca aacggtgcga
    11941 gcgccgcctt gagacgtgcc tcgctgatca gcggttccag gcgcttcacc cggaagctga
    12001 gattgttctg gagtgcgcgt attcggcact cctgcaggga aaggcgaacg tattgctctt
    12061 gtgaggccgg tatagcctca aaggcaaacg cgttgaggat ggcaacgaaa acacactcgg
    12121 cgactgcgcg gcttttcatc gatcactcca gttgggaaag cagcttcgcg tgcttcggaa
    12181 cagcggcgga gggggccgac tcggattgta tcccacgtgt ggtgaaaaat cgcgaaatgc
    12241 cccacctgaa tcaacgcgcg gaccgaccgt tttcgtatga attgcgcttc atccggcggt
    12301 tcggcggttc cgggcatcgc gaatgattcc gtttccggtg ccggggagtt atctttattc
    12361 agcaccgtag gaggaagact cccgcaggat cactgccggt ctcctctcgt tcgattggac
    12421 catcgagtat accccagatg ctgaaaatca ccaagaaaca gcgtgagcgc tggccgcgcg
    12481 aactgattct ttccgaaaag agtgaagagc ttgaggagca tctcacgctt ctgaatgcag
    12541 aggaccgcgc ggccgttgaa cgggtttccc gggaggtcga ctccaaaatc gtcgaagcgg
    12601 cgcgggaatc cgtgaaagaa ctgaaaatgc tggaggaagg ccggtgccct gcctgcagcc
    12661 agaaaacgcg ccgttttctc ttcagcacca tctgcgagca ttgcggttgg tcagggtatt
    12721 cccgccccct gcgtgcaaag gtcgtcattc accttcgtga cggatccgcg ctcatctgcg
    12781 aggatgtgta ccacactcct gacgaactca tgtgtgtgac gcaggacgtg gttcgttacc
    12841 gtgttccgcg aaacgttgtt gcgtacgtcg aattcgatca cgccgaagaa gacattcggc
    12901 agcggcgcga gcaactcgca agagaagaac agggacaatg ttcgtggtgc atgaaaccgg
    12961 ttgtgcgaac aaacgaggat acgagagtca cgttcgcggc gttcgggatc tatcaggaac
    13021 ggtttttttt ctgcagtatt cactgccaga ccgcgtttca gaagcaatac ccgacccgca
    13081 tccatcggga ttgctaccac cgcgattgca gcaagtgtga ggagtgccag aaacgattcg
    13141 acgatacgtc gtatgagacg tttctggaag aggagttcgt acactgagtt cgtcggcgga
    13201 ggccgcgttc tcaatttcgc atcatgagtc gggggataag gcgctccgac aggccatctt
    13261 gaacgtcgga acaaacgaaa ccggccggaa caggcgcttg tgctgggcca gtcccggttt
    13321 ggagaggtct ccggcatcgt tcacaagatc gcaaccttcc attcgttccg cgagcacaag
    13381 tcgtgtagct tccgcgaggc ctttgcaggc tggatcgctt ttcagaacga aaaactgccc
    13441 cagaccttcc cagatcatgc cgccgaatgc aaacgcccgc actttcccgc ccaccacagc
    13501 tacgacgcca accagatgcg gcgggcggaa tctcgccgcg gaccgtatcg cactcagcgt
    13561 gtacccaaaa tcgaaaaccg gcacggggtc ctgacgatgc atgtcctgcc actcgcggag
    13621 aagcgcctcg cactccggaa cgtctgactc cacaaatgca cggaactcag gctggatttc
    13681 ccgttcacac cggtggattc ttttcctgag agcacggaac tcgttcccgc gaagttcaat
    13741 caatgccctc cgctggtaga catattcatg ctcgtagggc gtgactaccc atccgcggcg
    13801 agatccgtat gcggctccag ggaggtcagc ccacagaaac cgtgtctcta tcgggccgtt
    13861 gaggcgggcg agagaacccg tcagattctc cagcgcgcct tcatagccag atcccagggg
    13921 aggcaaaagg aaatcgatgt ggccgtgccc gcgaactgcc cgatgcagga gaacaaagag
    13981 gcttccgccg tgttccccga gcatgaccgc acgggccggg gagtcggaat aggccagcag
    14041 aaaaggaaaa tacgccgccc agagccacgg gcgcgcgttg cctgcatagg aaagaaacgt
    14101 tggcatctgc gcgatttcgg ccgcgtggaa gacggttcct ccgatttcaa gcggggcgtg
    14161 caggtgatgc cggtcaggct ggttccccat cgccggctgc ttcacttgaa cgcgccaacg
    14221 ccgcggcggc agatctcaat tcgttctgat gacgggcgaa aacgatttct gaggacgtcc
    14281 accgcggcct ccgtgtcaat cgctccgcag gcgaagatat cgatcgacac aagtctgcat
    14341 tcagcccagg tgtgaatact cgcgtgggat tgagcaagta tcagtatccc cgaaaaacca
    14401 accggagaaa actggtgcgt gtgctcggaa agtatggttg ccccggccgc cagaactgat
    14461 tcgcggagaa aactcattga ggggtcggga gtgttcagaa ggaccgggtc atccaccaca
    14521 aagtcgatga gcagatgaac tgggggcgag caggtcaatt ttgtgctcct ccgggttttt
    14581 caaattccgc ccgagagtac tggcgggcac gcaggaagcg atcgcttcgg cacagggggc
    14641 aacgatcctc cttcagtctc tccacagtaa tccagcgttt gccttggcct acctcccact
    14701 cggcggaaca cccgaggcag cggtacacgc gaattactgg tcgcacatcc gccgtataca
    14761 acggcccttc gtaagtctcg cgcagggtag gagcgatctc cggtgtggcg atcaggtgag
    14821 ccataaggga aacattcgcc aacacggagg acccctcgta ttcgttgaac gcaggaatcc
    14881 actcgacgat tacccagccg atacccgaaa ttgccgcttg aacctgcctc atcccgcccg
    14941 ggtccagatg ccccatggaa aagaaacagt gtgctccttc gtgctctacg gcctctgcgg
    15001 cgcggcagag gaacagctcc gcgccccccg gggtgtacgg aggatccgtg aaaacgacgt
    15061 caaatcggtg ctgaagggag tcgggtaacg gtttgcgaag atcgtgaaca aaggtttgca
    15121 ccgaaggcgc agcctttttg atcccgtcgc atacgcgtcc gtcgatatcc acaacggtta
    15181 gcgttggtgt cgggccggcc ggttccccac cggcgcactg atggacccat ctcgcgaccg
    15241 cgacagagac aaaatcgtcg tcgccaagaa agagaatccg gcgaccccac agggaatcgt
    15301 tcttcagaag atagttcaca cgccggacga gggttccgac ggtagccttt gcctggtcca
    15361 gggtcacgtc agcctgcggg cgcagagcgg cgagatcatc caacacggcg gagagcccgg
    15421 ttgtctcgtc atgcccggcg tcgccacaga tagtctccac acacgaagcg cccgctgacg
    15481 taagcctcac tccgttcgga gcccgttccg tcatcccgcg ggcggagagc tcgccgatga
    15541 cggccacggt gacagggagc ggcaggcgga ccttcgaagc gagaactcga acgggaaccg
    15601 gctccagtgt ggcgatcgct cgaaggattg tgcgaacgcc ctcacgacct tcccgaagat
    15661 gcgaggcaga cgctacctct gacaggattt ggccgccgtc gccggcgtga agctgctcat
    15721 gaccttcccg gagcgccata tgtgcttcca aaagagaacc cgcgcgtgcc ggtgcccggt
    15781 cggtcctggc gcgaggatcg cctttcccgc tcttgaggga gtgctgcggc cctgttggcg
    15841 cgtgcctgct cgcgttcaat gtcgtcctcg gtctggagcg ctgcgcgcca ctcgtcgaag
    15901 cgacttgatg cgaacagcgc agccaaccct ccggcgatat ctgcgaggga ttcctgttcc
    15961 tgtctccgcg cgccgcgcgc cccgaactgg cccccctgtg gttcactgat cattttgcgt
    16021 gccagtttat ccgaggttct gatatcgagc aacggcaggc ttcctgcgcg cagtgtgttc
    16081 acgaacttta tgaagcccgg caattcggtg cgacgcacgc gcatgttgcc gtctcctcga
    16141 atgcagagaa tcggcctgtc ggggagattc atcagaaggg gatattgctc gcgaaggcgg
    16201 ggccaccagt acgtccagta ggacggcgga gctatggtaa taagacggtt cctcacgttc
    16261 gggttcgaca ggtttgaatt gtcgaacaca aacgacagcc tcggtcgtat gccctgctcg
    16321 tcatttccga acgggaagag agttaccccg cacagtctga gtcttcgttc gatgatacgc
    16381 gcttcgccgg gctgcaggtc caggttgatg aaagcaccgg tgagttcatc gggcgctatc
    16441 tcaaactccc aaccggctac ctgggcaagc gcgaccagtt cgtcccgcac ctgtgcctgg
    16501 cgcgatagac tgaattcctg tgcctcccag aaaatcgtgc atcccatggg aatctacttt
    16561 cctctcgtga cggcagatgc cgacttcggc ccggaaacac gagcgaagcg gcgccagtga
    16621 acgatctgtc tcgcgagatt gcgtgttgaa aagcaaggga aaaggaagta atcaaagata
    16681 acctctgagc gggaactcaa caaggtgccg tcatagcgct gtacacccag tccaagggta
    16741 caggtatttt tgcgactccc aattccctat cttttactgc aaagtatggc gggaagcgag
    16801 aatcaggttt cccgcggagc gcgactttcg cgtcgtcctc ttcgtgccgg caggaggttt
    16861 gtggttatgg tttcggatgc agatttgctc gacaagatta ccgattatac ccggaaacag
    16921 ggcggagaca cggcaatcga gagacttctc gcgaatgtcg tatcgcaggt gcgtgaattc
    16981 gcggaggccc gggcttcaga aataaagcgg ctctcggaca tcggaattgc cctttccgcc
    17041 gaacggaacc tcgaacgctt gcttgagcgc atcgtggacg aagcgcgacg atttacgcgg
    17101 gctgacgcgg ggactctgta cacggtccag atcaatcctg cgaccaacct gccggagctg
    17161 aagttcgata tcatgcagaa cgacaccatg aacacccggc agggtggaac gagcgggaat
    17221 ccgataacgc ttcctccggt gccactggtc aaagacggaa aaccaaacca caacaacgtt
    17281 tcggcgtacg ttgccgacac cggcgaaacg gtcaacattc ccgacgtgta tatcgcggag
    17341 gggtttgact ttaccggccc gcgcaagttc gatcagatca ccggttaccg aacaaagtcc
    17401 atgctcgtgg ctcccatgag gaaccacgag ggagagatta ttggcgttct gcagttgctg
    17461 aatgcgaaag ttccgggcgg cgaacgagtg attccgttct cggacgagta cgtggatctg
    17521 atcaagagcc tcgcgtcgca ggctgcggtc gcgatcacca ctgccaggct cattcaggat
    17581 ctccagaacc tttttgagtc cgttatccgc ctggttgcct ccgcaatcga cgagaaatcc
    17641 gcgtacaccg gcggacacat cactcgcgtg gcggggttgt cgatgaagat cgccgaagcc
    17701 atcaacgaga cgacggaagg ggatttcgcg gatgtgaagt tcacacccga cgaactgaac
    17761 gagcttcgga tcgccgcctg gttgcacgat atcggcaaga tcaccacacc ggaatacgtt
    17821 gtggacaaag ggacaaaact ggagacgatt tttgaccgga ttgaactcgt caaaacgcgc
    17881 ttcgaggtca tcagacgcgg cgtgatcgca gatgccatgg agtacaaaat gcgtcttatt
    17941 gcgaatggcg ccgatcccga ggctttgaaa gcggttgacg agcaactggc agtaaggctt
    18001 gaggagatcg aaagcgataa ggagttcgtt gcgcgctgca acacgccggg cgagttcctc
    18061 aaggatgacg cgatagaccg gttgaaggca atcgccgcga agacgttcct gcttgacggg
    18121 gtggaaacac cctacctcac cgagaacgaa ctgaagaatc tgagcatccg caaagggagt
    18181 ctcaccgaag aggaacggca gatcatcaac aaccacgttt ccgtcacaat caaaatgctt
    18241 tcgcaattgc cgttcccgaa gaaactttcc catgtcgtgg agtacgcagg tgcccaccac
    18301 gagaagttga acggtgccgg gtaccccaac gggtggaccg cagagcgcct ggcaatccaa
    18361 tcgcggattc tctgtcttgc cgacatctgt gaagcactta cggctgcgga ccggccttac
    18421 aaaccgtcca tgccgaaaga acgcgcattt cagattctcg gtttcatggc caaagatcag
    18481 gaaattgatg agaggctttt gaagttgttc ctggagaaag aggtctatga ccgctacatg
    18541 gtggaataca aagcgcagaa ggaagcagaa gccgcggcag ccacggccaa atgaagagcc
    18601 ggctttccga atgtgaccgg aaagtcgagg gacgcgatga cgagcggatc ggtgaacccg
    18661 atccgctttg tcatgatggg ttttcagggg aatgaacaga cagagcgaag gagattggaa
    18721 caatgtcgaa accacgtctg gggttcatcg ggctgggcat aatgggacgt ccga
//
LOCUS       DAOSOO010000045        18325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3046_length_18325_cov_4.768692, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000045 DAOSOO010000000
VERSION     DAOSOO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18325
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3046_length_18325_cov_4.768692"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLT86_05080"
     CDS             complement(<1..680)
                     /locus_tag="PLT86_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36018.1"
                     /translation="MKRLNNLVVELASHAKLESGDVVKFRTPREGWVFISCEGKKPET
                     RQLRINGRPHPWKSSLAGNLEAMCYLPQGEHVLAVHGKPARRLAIRSIPELLFTKYGY
                     NPFVKDYGPYDWQFLKRYVLPHVNTVIGTGSPEQEAQNREWRSAGGKWIVERGISFDK
                     SARPDAGEVVSSWGGEPGMADPAFDGLLLDEFFSGDDARYPAFSEAVHRLGADPRFKA
                     KKLYPYCG"
     gene            complement(736..3939)
                     /gene="carB"
                     /locus_tag="PLT86_05085"
     CDS             complement(736..3939)
                     /gene="carB"
                     /locus_tag="PLT86_05085"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOT36019.1"
                     /translation="MPRRDDIKKVMIIGSGPIIIGQACEFDYSGTQACKALRRLGYQI
                     VLANSNPATIMTDPGMADFTYIEPLNKDTMEEIIRKERPDALLPNLGGQSGLNLSSEL
                     AAAGVLDKYGVRVIGVNVDAIKRGEDRLAFKQTMESLGIDMPKSEIANSVEEAEEIAQ
                     RLDYPLVVRPAYTMGGTGGGFVYNLEELRTIVARGLAASLVGQVLIEQSVLGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSFCTAPMLTISPELQLKLQDYSYRIVEAIE
                     VIGGTNIQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAYVSSLLAGGLTLDEI
                     PYWREGTLEKYTPGGDYVVVKFARWAFEKFKGVEDRLGTQMRAVGEVMSIGKTYKEAF
                     QKAIRSLETGRWGLGFAKDFNSRSLEELMEMLRWATSERQFIMYEALRKGASVAELHE
                     RTYIKHWFIEQMKELVLLEEELLRFKGSLPPDDLLIRAKKDGFADRYLAKILKVPESD
                     IRRRRTELGVVEAWDAVRVSGATDAAYYYSTYNGEDRVPVTQNKKKIMVLGGGPNRIG
                     QGIEFDYCCVHAAFALRDAGYESIMVNCNPETVSTDYDTSDKLYFEPLTLEDVLAIYE
                     KEKPEGVIVQFGGQTPLNLAAELAANGVRILGTSPESIDLAEDRDRFRMRMRELGIPQ
                     PDSGMAGSVEEAVRVAAEIGYPLIVRPSYVLGGRAMEVVHDEEMLREYVAKAVDISPE
                     RPILIEQFLQNAIEAEADAIADGTDAFVPAVMEHIEFAGVHSGDSACVIPPVSIPQKH
                     IDTIEEYTRRIAVEFKVVGLMNVQYAIFDDTVYILEANPRASRTVPIVSKVCAISMAN
                     LATRLMLGAKLPDLNLRRRPIPHFGVKEAVLPFNMFPEVDPILGPEMRSTGEVLGIAE
                     NAGLAFFKAEAAATPPLPLHGTVLITVTDPDKPGALEAARRFVQLGFSIRATEGTHRF
                     LTENGVPSTPILKMREGRPNIADAIKNREIHLVVNTPGGKRSLTDDSYIRKAAIKYKV
                     PYVTTTAAAIAAAKGIAAWRQGRGEVKSLQAYHADIG"
     gene            complement(4147..5064)
                     /locus_tag="PLT86_05090"
     CDS             complement(4147..5064)
                     /locus_tag="PLT86_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36020.1"
                     /translation="MPEQPILLVCNKHDDTMSFVDLTSYEVLETIPTGPNPHEIVVTP
                     DNRWAYLSNYAPPGNTISLIDLVAAKHVRQISTGEYSRIHGAAMAPDGRWAYFTAGQK
                     GYVVELDTRTNEVTRAILTCGKISHMVLVSPEGDFLYTANIVTKDVSVLRRTDGALAA
                     LVPCQEGVEGLGITPDGRELWALNQESGTITVISRPSHKVAETFPCPGMPVRVAFALD
                     GQLALVPSWTEHGELIAIETAIRREVGRVAVGSQAIGIVLSPEGDRAFVGCEHTDGVH
                     VVDIHKLAVVERFFTGNGSDAMAVWYPPR"
     gene            complement(5109..6239)
                     /locus_tag="PLT86_05095"
     CDS             complement(5109..6239)
                     /locus_tag="PLT86_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOT36021.1"
                     /translation="MQERRTRVIYNCDADNMFIYDYPMTVDNVNRYVDEIVDTDITTF
                     FMCCNYGMDMNFRGETGQLIGDRLTAEQVEALKDPAVNKPASFQRGILNFRQLIQQGH
                     DPLGMVISRAQEKGCETFVTFRLNEVHCVDEEPDSVLLSKFWLEHPEWRVGKVGDPLP
                     DLHKTILGPVHPIVASWLPAGLDFAVPEVRDYVLSQLTEVCERYPLDGLDLDFQRFPI
                     YFRFGQEASNLPVMTEFVRSIRSMTNRVAGRRGRPILLSARIMETPEQNLGLGLDPVS
                     WAREGLIDFVTISHYLRNTHPLPIGDFRRLLPSDLPIYGSIEFEREPDRYREIARQLW
                     KDGADGIMLFNFFCTREGGAELMFWLLNEIGRREKMLDGSNT"
     gene            complement(6252..7187)
                     /locus_tag="PLT86_05100"
     CDS             complement(6252..7187)
                     /locus_tag="PLT86_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT36022.1"
                     /translation="MRVCRFWEPKTGARLGIVFEETVVDATAIDPRGCADISTWLALP
                     DPIEYVRGITELAERSKNRLHIRELEHAPAPGSRHLLPPIDKQEIWGAGVTYQRMRQS
                     HVDESHGGKLFYDEVYDAARPELFFKTTPHRVAGPFGSIRVRSDSEWTIPEPELTVLL
                     SPAQRLIGFTCANDVTSRDIEGENPLYMTQSKTYSGCCALGPTILVSDDPDFARDLPI
                     SLVVIREGMAVFRDETNTNQMKRSIPEILGWLSRDNDFPFGVFLMTGSGIAPPDDFSL
                     EPDDIVEIAIDGIGRLVNPVVKDALPSETQTEFGV"
     gene            complement(7387..9294)
                     /locus_tag="PLT86_05105"
     CDS             complement(7387..9294)
                     /locus_tag="PLT86_05105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT36023.1"
                     /translation="MLRFMRNQRFVKALMWIVILSFVGWLGFELGYGGGGRSQATPEV
                     GEIAGEPVYWTEFRREIGTLREYYRRQGNTQIDDFDLDEQVWQQTVQRIILRNAVERA
                     QISVTADELAEELVNNPPSGFERVPEFQRADGQGFDREKYRQFLASMTQERWFQITGM
                     TYSEYESRVLFDLKVQKLQQRIDDNGWITDASLRQAFAEENETIRARVIAAPISLVPD
                     SLIKVSDDEIRSYYQTHLNEFKQRTRARFSYVVIPRSPSPRDVARAAEEIRAAYRQLQ
                     EGADFAETAQRLSEDEGSARDGGDLGTFGRGRMVPAFDSVAFSLPVGEFSQPFHTQFG
                     WHIVKVERRVEDTAWQQQWGRLKAQKKPLPPEAGTPRDSVQARHILIKDTEPSFETSD
                     SLQAIAEKIAAAGPRFATTAAGYGLTVETTPWFTEDVNSPVPAVQDPLRRLVRWSFSG
                     TVGSVASPAYTRDHIVVARLADRSPEGPQPLREVRDAIALKVRVEKRVDIATQWLTPI
                     AKKIAAGQPMEQAVQGTEFTVAEIGPIRRGDFVPEVSAGASDGFTGAAFALTTTGQTT
                     GALRIPERGSYIVRLLEKSLDWSTFDQQREQLRQRLARQRRQSSFRDWETYARKSADV
                     KDYRDRFYNFD"
     gene            complement(9403..10623)
                     /gene="ilvA"
                     /locus_tag="PLT86_05110"
     CDS             complement(9403..10623)
                     /gene="ilvA"
                     /locus_tag="PLT86_05110"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOT36024.1"
                     /translation="MAKIPTIDDIHAARERISKYAYVTPLEHSTDFSRLLGNDISHKL
                     ENLQLTGSFKVRGALNAVLSLPKGELAAGVIAASAGNHAQGVAFGAHQLGIKATIVMP
                     LGTPLVKLMAVKRWGAEVVQTGETYDEAADCAASMAHDTGACLIHPFDDPKVIAGQGT
                     IALEILEQKPDVDAVVVPVGGGGLISGIGIALKELHPRVQVIGVQASGADAMAQSLGA
                     GEIRKSPRTLTFADGIRVARPGDLTFAISRKVVDQVVTVDDDEIANAVLLMIETDKSV
                     VEGAGAAPLAALLHKRVGLRGKHVVLIVSGGNIDVNILSRIIERGLVKTGRLIRISVT
                     LGDHPGALAGITDHLAKFGANVLHVHHNRAFARGRLDETTVEFALETRGHEHAREIVQ
                     GLSRAGYLCDVLAG"
     gene            10787..11305
                     /gene="pyrE"
                     /locus_tag="PLT86_05115"
     CDS             10787..11305
                     /gene="pyrE"
                     /locus_tag="PLT86_05115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT36025.1"
                     /translation="MELSTLAKAIKEAAYLEGDFVLSSGKRSRYYLDKYLFSTEPRLL
                     EAIAEALSALLPQDFDRLAGVELGAVPLATAVALRTGKPFVIVRKNAKDYGTGKLFEG
                     RLAKGDRVVLVEDVLTTASQAIQAARRLNEFGAEVVKIIYVIDREEGAADNLAAAGFS
                     AEPVMKKSDLGL"
     gene            complement(11344..13569)
                     /locus_tag="PLT86_05120"
     CDS             complement(11344..13569)
                     /locus_tag="PLT86_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36026.1"
                     /translation="MKSWLDEVDRWVREIVHASTHGRAYDLLVGDPLPEEWGGQAAIA
                     SYLAGCLALHGKEKNDATQVERSREFMLLPDPVHAFAASPAWEAIVALRAAHMLSDTN
                     EEQIRAAMTKTAQNGVRNYLVIRMGIRRFNHAITTANMCDVISRLWPDHPDSAELRQA
                     AEDVWGDWWLIGDNIEIAANYEAFAQVDLLLWAERRGEKARMLDDPMTHFWMDRCLEH
                     MLPVGIVPGYGDTCTMELWADWFALYSLMAAWCGGLRGSRAAWNAERMFSWATTRGWL
                     DQNLSIIDEVPDDPYRAKQGWTQMPRTGWYLALGRECLRKACAEPTAPPLRPVVTHRT
                     VFAQHVMSDLSWSLTPPAGGPRVADKIVLRTGVEADSPSLMLGCARQVWHDHLDAGAV
                     IAFVSDGTVHLDDTGYMQKYPVFHNLFWADAREKTWLHYGKEEYSRHRELHRPSDYRV
                     SALTGREVAQLAVIDCEAPHCMPIYHRRETILTRGGVTIIADHVEPYTKGLVGSPVWH
                     VQYVHESTHDWAEVSIGEFRGMNGQHCDNAAGRMRILTPTHGDPWTIIQQENNDPYAS
                     PGYREPVTRYFDYWKRSFVLRTALSKPRTLTTDAPNRFYTVLVPLPPSHGQPGGNLVE
                     LLGEEIDLGFVARLGDGIVSLNCTEGQLMGNWGSTDAMHMWWDGKGVFAHRVKAINLP
                     ELRLDTGGQWADLDLFWDNEGISGVISTERATTVTVEVPGTRASFRVHGITEVGRRTD
                     R"
     gene            complement(13583..14770)
                     /locus_tag="PLT86_05125"
     CDS             complement(13583..14770)
                     /locus_tag="PLT86_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT36027.1"
                     /translation="MSLHQRLCDAVNEARIGQTALELVSIPSPTGFTREATRYFETAY
                     RELGCEITRIDGIKGSPSGTDAPSVAARLRGTGAAPTLQIDGHIDTIPAAHAPASLVD
                     NVLSGRGASDMKGGLAAVMEAARVLREVRVQLPGDLLLTTHGLHEAPTGHGEGLLALI
                     SEGLHGAAAVVAEGPVDELAITGRGMAIFTITVKTTGKSGHENETPPNTPHPLVAAAE
                     VLRVLEAERVRLQRTMRPYVGSETIFFGQLHGGDFYNRFPVEATIQGTRRYFDDHDFQ
                     DVRKEMEAILARVAEDTGTTITLDLDRIRDGFRVSENERIVHAFRWSYRTLNGKEPPL
                     GAFKSVADVSLLVQAAGIPAVFCGNRGTGAHADREEVDVRELVHQTRMLLGIIVAYFG
                     LET"
     gene            14897..15853
                     /locus_tag="PLT86_05130"
     CDS             14897..15853
                     /locus_tag="PLT86_05130"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT36028.1"
                     /translation="MSESSLMRAARVAVGQCLGVQKSEQVIVIADAPCLSIGRALFEA
                     SVETGADTQLIEILPRAENGSEPPPPVAAAMAAAHVVFAPTSKSLTHTEARRAASEAG
                     ARIATLPGITEDIMSRALLADYEAIAERTQRVAIALQGKKTIRVTTAAGTDISFSIEG
                     RKVSADTGILRNPGVTNLPAGEAYLAPVEGTGHGLIVIDGCIGSSGVLREPIEVVVEG
                     GMATIIRGNVYAETLVARMDAAGPEARNLAELGIGTNDAARLTGQVLEDEKILGTVHL
                     AFGDNRSMGGIVRAPFHQDGILLRPTVWADGCLILDDGVPMV"
     gene            15873..16697
                     /locus_tag="PLT86_05135"
     CDS             15873..16697
                     /locus_tag="PLT86_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT36029.1"
                     /translation="MTENRVMEAFLVVWKYPLFTLGGGTLTLAVLTKTILGLVFVWVV
                     ARVIRWKVTAGTFAKGRIESSTRYTISRILYYTVLFFGVVIVLDTAGLHLSSLTVFSG
                     LMGAGIGFGMQNIASNFISGIILLVERPVRSGDVISIGDTRGVVDSISIRMTRVVSFD
                     NTILLIPNSRLVNEMLVSWTTPSYQFRSSLNVVVPNKHNPARVKELLLEVAGRDSRIL
                     PDPAPAVRFKDMSATTQTYELVVWTRQSSSASEVPSDLRFAIVAHFEENGVSLVTG"
     gene            16766..>18325
                     /locus_tag="PLT86_05140"
     CDS             16766..>18325
                     /locus_tag="PLT86_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36030.1"
                     /translation="MPAYTTKNLPLTYLINYQDHLGDIEASVAPLRDAPPNVFHACND
                     MPYLSRAGASSYHDRKGPLAWSFPIGIEEVRERTKATVKWVNAVHAAGVDVVIPYICN
                     QTIGGDPEKRLGFWWFFDRWDDYAEDVGPKPGIDPIEWMQREEDGSLHFNYPYRFTRT
                     DPPLRFAPCPNNPFWHSWLKTMVRLIASYGYDGTFVDNNIMHCRCKYCQQEFAVFLSE
                     TYTKKELKDRYGSTDPSRPKLATAGDVIYWACRQPEYVRQFRDKEPELFKEIFGTTDV
                     RKAKMSEGGTGLHWMKSYKFWLATLERTLGEEGAEHARRVGDVSMLGVRNPRMLCRWA
                     DTQKFWAWTIARRNAELREAAHEVTPRFLIVPNWGNMTGFCHALSRCRDAKNVRLWAR
                     GTDLIFFEDEFFPGTLAPGYTLDLMISYKYAAACGIRSCVLPYQGAESRNLTELATAE
                     AVAFSGDGAFVQMPYHFSEIRQAYRAFFEGNPHLFRGRSSYANVGLLLSFDEVHMENT
                     HHLRESAACARY"
BASE COUNT         3713 a         5378 c         5211 g         4023 t
ORIGIN      
        1 tacccgcagt acgggtacag ctttttcgct ttgaacctcg gatccgcccc cagtcggtgc
       61 accgcctcgg agaaagcggg gtagcgagca tcgtccccgc tgaagaactc gtccagcagc
      121 agaccgtcaa aagccgggtc cgccatcccg ggttccccgc cccacgaaga aacaacctca
      181 ccggcgtcgg gacgtgcgct cttgtcgaag cttatccctc gttcgacgat ccatttcccg
      241 ccagcgctcc gccattccct gttctgggct tcctgctcgg ggctgcccgt gcctatcacg
      301 gtgttgacgt gaggaagcac gtatctcttc aggaactgcc aatcgtaggg cccataatcc
      361 ttgacgaacg gattgtatcc gtacttcgtg aacagcagtt ctgggatcga tctgatcgca
      421 aggcgtcgag caggtttccc gtggacggcc aatacgtgct caccctgggg gaggtagcac
      481 atggcttcca ggttgcccgc caaggagctt ttccacgggt gagggcgtcc gttgattctc
      541 agttgccttg tttccggctt tttcccctcg caggatatga agacccagcc ctcgcgaggg
      601 gtgcggaact tcaccacgtc gccggattcg agtttcgcgt gcgacgcgag ctcgaccact
      661 aggttgttca accttttcat tccgacctcc cagcgagtgc ccggctctac tcagaatccg
      721 tttcagagcc cgggatcatc cgatatccgc gtggtaggcc tgcaggctct ttacttcccc
      781 acggccctgc ctccatgcgg caattccttt tgccgcggcg atcgcggcag cagtcgtagt
      841 cacgtaagga actttgtact ttatcgctgc tttgcggatg taagaatcgt ccgtaaggct
      901 ccttttgccc ccgggcgtgt tcactacgag gtgtatttcg cggttcttta tcgcatcggc
      961 aatattcggg cgtccctcgc gcatcttgag gatgggagta gacggtaccc cgttctccgt
     1021 caagaaacgg tgcgtgcctt cggtagcgcg tatggagaac cccagttgga cgaatcttcg
     1081 ggccgcttcc aacgcccctg gcttgtcggg atcggtgacg gttatcagca ccgtaccgtg
     1141 gagcggaaga ggcggcgtag cagcggcttc agctttgaaa aaggccagtc ccgcgttttc
     1201 ggcaattccg agaacctctc cggtggatct catttccggt ccaaggatcg ggtccacctc
     1261 cggaaacatg ttgaacggaa gtaccgcttc cttgacaccg aagtgcggga tcgggcgcct
     1321 gcgcaggttg agatcaggga gcttcgctcc cagcatcagc cgcgtggcga ggttcgccat
     1381 ggagatggcg cagaccttgc tcacgatcgg aacggttctg ctggcacgcg ggttcgcctc
     1441 aaggatgtat accgtgtcgt caaaaatggc atactggaca ttcatcaggc caaccacttt
     1501 gaactcaact gcaatccgtc tggtgtattc ctcaatcgtg tcaatgtgtt tctgggggat
     1561 gctgacgggc gggatcacgc acgcggaatc gccagaatgc acgcccgcaa attcaatgtg
     1621 ctccataact gccgggacaa atgcgtccgt tccatctgcg attgcatctg cttccgcctc
     1681 gatcgcgttt tgcaggaact gttctatgag aatcgggcgt tccggggaga tatctacggc
     1741 tttcgcaacg tattcacgca gcatctcctc gtcgtgcacc acttccatcg cgcggccgcc
     1801 gagaacgtag gacgggcgca cgatcagcgg gtacccgatt tcagcggcaa cacgaacggc
     1861 ctcctcgacg gaaccggcca ttcctgaatc aggctgcggt atgcccagct cccgcatgcg
     1921 catgcggaac cgatcgcggt cttcggcaag gtcgatggat tctggcgacg ttcccaggat
     1981 gcgcacgcca ttcgcggcca gttcggcggc aaggttgagt ggcgtctgcc cgccgaactg
     2041 aacgattacg ccctccggct tctccttttc gtaaatcgcg agaacatcct cgagtgtcaa
     2101 cggctcgaaa tagagcttgt cggatgtgtc gtaatccgta gagacggttt cgggattgca
     2161 gttcaccatg atggactcgt acccggcgtc ccgtaaggca aaagccgcgt ggacacagca
     2221 gtaatcgaat tctataccct gtccgatcct gtttggccca ccaccgagca ccatgatctt
     2281 cttcttgttc tgtgttaccg gtacgcgatc ttcgccattg tacgtggaat agtaatacgc
     2341 cgcatccgtc gctccgctca cgcgtacggc atcccacgct tccacaacgc cgagttcggt
     2401 tcttcttctc cggatgtcgc tctccggaac tttgaggatc ttggcgaggt accggtcggc
     2461 gaaaccatcc ttttttgcac ggatgagcaa atcgtccggc ggcaacgacc ctttgaacct
     2521 cagcagttct tcttccagga gaacgagctc cttcatctgt tcgatgaacc agtgcttgat
     2581 gtatgtgcgc tcgtggagtt cggcaaccga ggctcccttt cgaagtgcct cgtacatgat
     2641 gaactgcctc tcgctggttg cccagcggag catttccatc agttcttcga gggaacggct
     2701 gttgaagtcc ttcgcgaagc ccaggcccca acggcctgtt tccagagacc ggatagcctt
     2761 ttggaaagct tctttgtaag tcttcccaat gctcatcact tcaccgacag cacgcatctg
     2821 cgtgccaagc cggtcctcga cgcccttgaa tttttcaaac gcccaccgcg cgaacttgac
     2881 gacaacgtag tctccacccg gcgtgtactt ctcgagtgtc ccttcccgcc agtaggggat
     2941 ttcatcaagc gtgagaccgc ctgcgaggag cgaagagacg tacgcgatcg ggaaaccggt
     3001 agctttcgag gcgagagccg acgaacggga ggtccgggga ttaatctcta tcacgacgac
     3061 gcgacctgtt ttcgggtcat gcgcgaattg gatgtttgtc ccgccgataa cctcgatcgc
     3121 ttcgactatt cggtaggagt aatcctgcaa tttgagctgg agttcggggg agatggtgag
     3181 catcggggca gtgcagaatg aatcgccggt gtgcacgccc atcgcgtcca cgttttcgat
     3241 gaagcacacc gtgatcatct ggttcttggc gtcgcggacg acttccagct ccagttcctc
     3301 ccacccgagt accgattgct ctatcagcac ctgcccgacg aggcttgcgg ccagaccacg
     3361 ggcgacgata gtcctgagct cctcgaggtt gtagacaaaa cctcctccgg tccctcccat
     3421 ggtatacgcg ggcctcacca ccagagggta atcaaggcgt tgcgcgatct cctcggcttc
     3481 ttcgacgctg ttggcgatct cacttttcgg catgtcgatg ccgaggcttt ccatggtttg
     3541 tttgaacgcg agacgatcct ccccgcgttt gatcgcatcg acattcaccc cgatgaccct
     3601 cacgccgtat ttgtcgagaa cacccgctgc tgcaagctca gatgacaggt tcaggcccga
     3661 ttgtcctccg agattcggca gaagagcgtc gggccgttcc ttgcgtatga tttcctccat
     3721 cgtgtctttg ttcaggggtt cgatgtaggt gaaatcggcc attcccggat ccgtcatgat
     3781 ggttgccgga ttcgagttcg caaggacgat ctggtatcca agcctcctga gagccttgca
     3841 cgcctgtgtt cccgaataat caaactcaca agcctgcccg atgatgatgg gaccggaacc
     3901 gatgatcatg acctttttga tgtcgtcgcg tcggggcata cctggtagct cctcagattt
     3961 tctgtgcatt cccggcaccc tgaaaacccc cgaacgtgct gcgcatgctc aggggtcagg
     4021 cggcaggatc gcatggtgcg aagaacgtgc aaaagcgggc tgagcgtggg aactcaacag
     4081 aatgaaaaag tgatcactca aatttccgga aaagacggga acgcgtcaac tgagtcattc
     4141 ggggcttcag cgcggggggt accacacggc cattgcgtcg gatccattcc ccgtgaaaaa
     4201 gcgctcaact actgcaagtt tatgaatatc caccacgtgc acgccgtccg tgtgctcgca
     4261 gccgacgaac gcacgatcac cttcgggtga gagaacgatt ccgatcgcct gacttcccac
     4321 cgcaacgcgc ccaacctctc tgcgtatcgc cgtttcgatt gcgatcagtt ccccatgctc
     4381 ggtccaactc ggaacgagtg caagctgccc gtcgagggcg aacgcgacac gaacaggcat
     4441 cccggggcac gggaatgtct ctgcaacctt atgcgagggg cgcgagatca cggtgatggt
     4501 gccgctctcc tggttcaacg cccagagttc gcggccgtca ggagtgattc cgaggccttc
     4561 aactccctcc tggcagggca ccagagccgc cagcgctcca tcagtgcgcc tcaatacaga
     4621 cacgtccttc gtcacgatat ttgcggtgta aaggaaatca ccctcgggtg aaacaagaac
     4681 catgtgggag attttcccgc acgtaaggat ggcgcgcgtg acctcattcg ttcgcgtatc
     4741 cagctcaacc acgtacccct tctgcccggc cgtgaaatac gcccaccgac catccggcgc
     4801 cattgcggcg ccatgaatgc gtgaatactc cccggtagaa atctggcgaa catgcttcgc
     4861 cgcaaccagg tcgatgagcg atatggtatt gccagggggt gcgtaattcg agagatatgc
     4921 ccagcggttg tccggcgtca cgactatctc gtgcgggttt gggcctgtcg ggatagtttc
     4981 aagcacttca tacgaggtca aatcgacaaa cgacatggtg tcgtcgtgct tgttgcatac
     5041 cagcaggatc ggttgttctg gcatgtgcga ggtcttcttt ctcgaggacg gagacgggtg
     5101 cgccgaaatc aggtgttcga tccatcaagc atcttttcgc ggcgaccaat ttcgttcaat
     5161 aaccagaaca tcagctctgc gccgccttca cgcgtacaga agaagttgaa cagcataata
     5221 ccatccgcgc catccttcca gagctgacgt gctatctccc ggtaccggtc tggttcgcgc
     5281 tcaaactcaa tggaaccgta gatcggtagg tcggacggca agagcctgcg gaaatccccg
     5341 atgggaaggg gatgcgtatt gcggaggtaa tgggaaatcg tcacgaaatc gatcagccct
     5401 tctcgagccc aggagacggg gtcgagtccc aacccgagat tctgttccgg tgtttccatt
     5461 attcgtgcgc tgagcagaat gggcctgcct cgccggcctg cgaccctgtt cgtcatggaa
     5521 cggattgacc tcacgaattc ggtcatgacg ggcaggttgg atgcctcctg tccgaaccgg
     5581 aaatagatgg ggaaacgctg aaagtcgaga tcgagaccat ccagagggta gcgttcacac
     5641 acctcggtca actgcgagag cacgtaatcc cggacctctg gaacggcgaa atccagacca
     5701 gccggcaacc agcttgccac tatcgggtgg accggaccca gaatggtctt gtggagatca
     5761 ggtagagggt ctcccacctt cccgactctc cactccgggt gttccagcca gaactttgac
     5821 aggagtacgc tgtccggctc ctcatcaaca cagtgtacct cgttcaatcg gaacgtgacg
     5881 aaggtttcgc accccttttc ctgcgcacgc gaaatgacca ttccaagagg gtcgtggccc
     5941 tgttgaatca attgccggaa attgagaatc ccgcgttgga acgatgcagg cttgttgacg
     6001 gcgggatcct tgagcgcttc cacctgttct gcggtcagcc ggtctcctat caattgcccg
     6061 gtctcacctc gaaaattcat atccatgccg tagttgcagc acatgaaaaa cgtcgtgatg
     6121 tctgtatcca cgatctcgtc cacgtaacgg ttgacgttgt cgaccgtcat ggggtagtcg
     6181 tagatgaaca tgttgtcagc gtcgcagttg tagattacac gcgttcttct ttcctgcatc
     6241 tgagagcccc ttcagactcc aaattcagtc tgcgtttctg atggtagcgc gtccttcacc
     6301 acagggttga cgagccgccc gattccatca attgcgattt caacaatgtc gtccggttcg
     6361 agagagaaat catccggggg agcgatgccg gaacccgtca tgaggaatac tccgaaaggg
     6421 aagtcattat cgcggctcaa ccagcccagt atttcgggga tactccgctt catctggttt
     6481 gtgttcgtct cgtcgcgaaa gacagccatc ccttccctga tcactaccag cgaaatgggg
     6541 agatcacgcg cgaaatccgg gtcgtccgac accagaatgg tcggcccgag cgcgcaacaa
     6601 ccgctgtatg ttttcgattg ggtcatgtac agggggtttt cgccctcgat atcacgggac
     6661 gtgacgtcgt ttgcgcaagt gaaaccgatg agccgctgag cggggctcaa aagcaccgta
     6721 agttcgggct ctggaatggt ccattcggaa tccgaccgca cgcgaatgct tccgaacggg
     6781 cctgccaccc tgtgaggtgt tgttttgaag aacagttccg gccgggctgc gtcatacact
     6841 tcgtcgtaaa aaagtttccc tccgtgggac tcatcgacgt gtgactggcg cattctctgg
     6901 tacgtaacac cagcgcccca gatctcctgt ttgtcgatcg gcggcaaaag gtgcctcgaa
     6961 ccgggtgcgg gggcgtgttc gagttcccgg atgtgcaggc gatttttgct tcgttctgcc
     7021 agttccgtga tgccgcgcac gtactcgatc ggatccggca gcgcgagcca tgttgagatg
     7081 tctgcgcaac cccgagggtc gatcgccgtt gcgtcaacca cggtttcctc gaaaacgatc
     7141 ccgagccggg cacccgtttt cggttcccag aacctgcaca ccctcatcag ccgctccttc
     7201 gatgtcccgg ggccaacaat ctgtgtggga cacgttcaca gtctggcttt gttccgtccg
     7261 aggcgcgtac acgagctaca atataggcag cgctgcgtga tgttgtaaac gttccacgga
     7321 gcaaaaaaag aaacgtgccc ggacctcgag aggtccagac acgtctttcc accatcctca
     7381 atgccgtcaa tcgaagttgt agaaccggtc gcggtagtcc tttacgtccg cactcttccg
     7441 cgcgtacgtc tcccagtccc ggaaacttga ttggcgtcgt tggcgcgcaa gtctctgacg
     7501 aagttgctcg cgctgctggt caaacgtgct ccaatcaagc gatttctcga gaagccgaac
     7561 gatgtaggaa cccctttccg ggattctgag cgcgccggtc gtctgacctg ttgtggtgag
     7621 agcgaacgct gcgccggtga aaccgtctga cgcaccggcg ctgacttcgg ggacaaaatc
     7681 cccacgccga atcggtccga tttccgccac ggtgaactcc gtaccctgga cggcctgctc
     7741 cataggttgt cccgcagcaa ttttcttcgc tatcggagtc aaccattgcg ttgcgatgtc
     7801 gacgcgcttt tccacgcgaa ctttgagagc gattgcgtct cgaacctctc gcaacggttg
     7861 aggcccttcc ggagaccgat cggccagacg agcgaccaca atgtggtcac gtgtgtaagc
     7921 cgggctggca acgctgccaa ccgtcccgga gaaggaccat cgaacgagac gcctcaaagg
     7981 atcctggacc gcgggcacgg gggaattcac gtcctcggtg aaccacgggg tcgtttcaac
     8041 cgtaagacca taccctgcgg cagtcgtcgc aaatcgaggc ccggccgccg ctattttttc
     8101 tgcgatcgcc tgcaggctgt cggaagtctc aaaactcggt tcagtatcct tgatcaggat
     8161 gtgacgcgcc tgaacggagt ctctcggcgt gccggcttcc ggcggcagag gttttttctg
     8221 tgccttcaga cgtccccact gttgctgcca ggcagtgtcc tcaacacgcc gttcgacctt
     8281 gacaatgtgc caaccgaatt gcgtgtggaa cggctggctg aattcgccaa caggaaggga
     8341 gaaggcaacc gaatcaaacg cgggtaccat ccgtccgcgt ccaaacgtcc cgaggtctcc
     8401 gccgtctcga gccgaacctt cgtcttcgga aagacgttgc gcagtttcgg cgaaatctgc
     8461 tccctcctgc aattgccggt aagccgcgcg gatttcctcg gccgctcgcg cgacatctcg
     8521 cgggcttgga ctacggggaa tcactacata tgagaaacgt gctctcgtgc gctgtttgaa
     8581 ctcattcagg tgtgtctgat agtacgagcg aatctcgtcg tcagacacct tgatcaacga
     8641 atcaggaaca agactgatgg gagcagcgat aacgcgcgcc ctgattgttt cgttctcctc
     8701 agcaaatgcc tgccgcagac tggcgtccgt aatccaaccg ttgtcgtcaa tcctttgctg
     8761 cagcttctga actttcaggt caaacagaac ccggctttcg tattcgctgt acgtcatccc
     8821 ggttatctgg aaccagcgct cctgggtcat cgaagcgagg aattgacgat acttttcgcg
     8881 atcaaagcct tgcccgtcgg cacgctggaa ttcaggaacg cgttcgaatc cgctcggtgg
     8941 attgttgacc aactcctccg ccagttcatc agccgtaacg gagatttgcg cgcgctcaac
     9001 ggcgtttctg agaatgatcc gttgaaccgt ctgttgccag acctgctcgt cgagatcaaa
     9061 atcgtctatc tgcgtgttcc cctgacgacg gtaatactcc ctcagggtgc cgatctccct
     9121 cctgaattcg gtccagtaaa ccggttcccc cgcaatttcg cccacttccg gcgttgcctg
     9181 agaccgtcct cctcctccgt agccaagctc aaagccaagc cagccgacga aggaaagtat
     9241 gacaatccac atgagcgctt ttacaaaacg ctgattgcgc atgaatcgta gcatcgaaca
     9301 gtcacccctg aacggcggga cacttacgac ctggagaaaa gacggcgtac aagactactt
     9361 aaaatatcgc gcgcggttag atatatcaac ccgttcgccg ggtcatccgg cgagcacgtc
     9421 gcaaaggtat cccgctcggg atagcccctg aacgatttcc cgggcgtgtt cgtgaccacg
     9481 ggtttcgagt gcgaattcca ccgtggtttc gtccagccgc ccccgcgcga atgctcggtt
     9541 gtgatggaca tggagcacgt ttgctccgaa cttggcgaga tggtcggtta ttcctgcgag
     9601 agcaccgggg tggtctccca gcgtgacact gattcgtatg agacgccctg ttttcacgag
     9661 gcctcgctcg atgatccggg acagtatgtt cacgtcaatg ttcccgccgc tgacaatcag
     9721 cacaacgtgt ttgcctctca atcccacgcg tttgtggaga agcgccgcca gcggcgccgc
     9781 gcccgcgcct tccactaccg atttgtcggt ctcgatcatc agaaggaccg cgttcgcgat
     9841 ctcgtcgtcg tccacggtaa ccacctgatc gaccactttt cgggaaatgg cgaatgtcag
     9901 gtcgcccgga cgggcgaccc ggatgccgtc cgcgaacgtg agtgtgcgag gtgatttccg
     9961 gatttcaccg gctccgagcg attgcgccat tgcgtccgcg ccggacgctt gaacgcctat
    10021 cacctgaaca cgagggtgca actctttcag ggcgatcccg atgccggaaa tcagtccgcc
    10081 gccccccacc ggaacgacga ccgcatctac gtcgggtttc tgttccagga tttccagcgc
    10141 gattgttccc tgtccggcga taaccttcgg gtcgtcgaag ggatgaatca aacacgcccc
    10201 cgtatcgtgc gccattgagg cggcacagtc agcagcctca tcgtatgttt cacccgtctg
    10261 gacgacttcg gcgccccacc tcttcacggc catcaatttc acaagcgggg tgcccaacgg
    10321 cattacgatt gttgctttga tgccgagttg atgcgctccg aatgccactc cctgggcatg
    10381 gttccctgcg gaggccgcta ttacgcctgc ggccaattcg cctttgggca gagacagcac
    10441 ggcgttgagt gcgccacgta ctttgaacga gccggtaagc tggaggtttt ccagcttgtg
    10501 ggagatgtca ttcccgagaa gccgggaaaa atccgtggag tgttcgagcg gggttacata
    10561 cgcgtatttg gagattcgtt cccgcgctgc gtgaatgtcg tcgatcgtcg gaattttggc
    10621 catcgtaatg aatgtcctgt tgcggagaga aagcttggca gggtactgtt taaattcgtt
    10681 gacaaatgcc cgtagtcgca agcggctgcc tcgaaaggcc ccatcaaggg acggcttgcg
    10741 gatgcgtgtt cgggaattgc gaccagtcac aaccaggaga actaccgtgg agctctctac
    10801 acttgcaaag gcgatcaagg aagcggcata tctggagggc gatttcgttc tgagcagcgg
    10861 gaaaaggagc agatactatc tcgacaaata cctgttttcg acggaaccga gactcctcga
    10921 ggcgatcgct gaagccctgt cggcgctgct gccacaggat ttcgaccggc tcgcgggagt
    10981 ggagctcggc gcggtccctc tcgctaccgc ggtcgcgctg aggacaggca agccttttgt
    11041 aatcgtccgc aagaatgcaa aggattacgg taccggcaaa ctgttcgagg ggaggcttgc
    11101 gaaaggcgac agggttgttc ttgtggagga cgttctgacc acggcgtcac aggcaattca
    11161 agccgcgcgc cgattgaacg aatttggcgc ggaggtggtg aagatcatct atgtgatcga
    11221 ccgtgaggaa ggagcggcag acaacctcgc ggcggcgggt ttctcagcgg agcccgtgat
    11281 gaaaaaaagc gatcttggtc tgtaaggtgg gggcaccccg gcgggggtgc cccggttcga
    11341 ccttcaccgg tcggttctgc gtcctacttc cgtgattccg tggacgcgga aagaagcgcg
    11401 tgtgccgggt acctcgaccg tcacggtcgt tgcacgctcg gtcgagatca cgccgcttat
    11461 tccctcattg tcccagaaga gatccaggtc tgcccactgg ccgcccgtgt ccagacgaag
    11521 ttcgggaagg ttgatggcct tcacgcggtg cgcgaaaacg cctttgccgt cccaccacat
    11581 atgcatggcg tcggtgctcc cccaattgcc catcagttgc ccttcggtgc agttgagcga
    11641 tacgatgcca tcgcccaaac gcgccacgaa accgagatct atttcctcgc cgagcaattc
    11701 tacgaggttg ccaccaggct ggccatgaga tgggggcaac gggacaagca ccgtgtagaa
    11761 ccggttgggc gcatcagtgg taagcgttct cggcttggaa agcgcggtgc ggagcacgaa
    11821 gctgcgcttc cagtagtcga aataccgcgt gacgggttcc cggtaaccgg gcgaggcgta
    11881 cgggtcgttg ttttcctgct gaatgatcgt ccagggatcc ccgtgcgtcg gcgtcaggat
    11941 tctcatgcgt ccggctgcgt tatcgcaatg ctggccgttc atgccgcgga actcacctat
    12001 ggacacctcc gcccagtcgt gagtgctctc gtgaacgtac tgaacgtgcc agaccgggga
    12061 accgaccaat cccttggtat agggctccac atggtcggcg atgatcgtga cgccgcccct
    12121 tgtgaggatc gtctcgcgcc ggtggtagat cggcatgcaa tggggcgcct cacagtcgat
    12181 aacggcaagc tgggcaacct ccctcccggt caacgcggac acgcggtagt cacttggccg
    12241 gtggagctcc cggtgtcttg agtactcctc tttgccgtaa tggagccacg tcttctcgcg
    12301 ggcatccgcc cagaagaggt tgtgaaacac cgggtatttc tgcatgtatc cggtgtcgtc
    12361 gaggtgaacc gttccgtccg atacgaacgc gattacggca cctgcatcca gatggtcgtg
    12421 ccagacctgg cgggcacaac ccagcatgag cgaagggctg tcagcctcca cccccgttct
    12481 gagaacgatc ttgtctgcca ctcggggacc accggcgggc ggtgtcaaag accaggaaag
    12541 gtcgctcatc acgtgttgcg caaaaaccgt tctatgggtg accaccggac gcagaggcgg
    12601 tgcggtgggt tccgcacacg ctttcctcaa acactcacga ccaagcgcca gataccagcc
    12661 ggtgcggggc atctgtgtcc agccttgttt cgcccggtaa gggtcatctg gaacctcatc
    12721 aatgatgctg agattctggt ccagccatcc ccgggtggtc gcccaggaga acatgcgctc
    12781 tgcgttccat gcggctctgc tcccccgcaa acctccgcac caggctgcca tcagggagta
    12841 aagcgcgaac cagtctgccc acagctccat tgtgcatgtg tcgccgtatc ccgggacgat
    12901 gccgaccgga agcatatgct ccagacaccg gtccatccag aaatgcgtca tgggatcatc
    12961 cagcatccgc gccttctcac ctcgccgctc cgcccacagg agcaggtcaa cctgcgcgaa
    13021 agcctcgtaa ttggcagcaa tctctatgtt gtcgccgatc agccaccagt ctccccacac
    13081 atcctcagca gcttgacgca attctgcaga gtccggatga tccggccaga ggcggctgat
    13141 gacatcacac atattggcag tggtgatggc atggttgaac cgtcgaattc ccatccgaat
    13201 cacgagatag ttgcggactc cattttgcgc tgtctttgtc atggctgcgc ggatctgctc
    13261 ttcgttggtg tctgagagca tgtgtgcagc gcgaagggca acaatggctt cccacgcggg
    13321 gctggccgca aatgcgtgta ccgggtcggg aagaagcatg aactcacgag aacgctcaac
    13381 ctgggttgcg tcattctttt ccttgccgtg cagagccaag caaccggcca gataggaagc
    13441 gatcgccgcc tgtccgcccc attcttctgg caacggatcg cccaccagga ggtcgtatgc
    13501 acgcccatga gtggacgcat gcacgatctc gcggacccag cggtcgacct cgtcaagcca
    13561 cgatttcata gtcctttgcc tttcacgtct cgagtccaaa atacgccaca atgattccga
    13621 ggagcatccg ggtctgatgg accagttccc gaacgtccac ttcctccctg tctgcgtgcg
    13681 caccagtgcc tctgtttcca cagaacaccg ccggaatccc tgcggcttga accagcaatg
    13741 atacgtctgc aaccgactta aacgccccga gcggtggctc tttgccgttc aatgtgcggt
    13801 aactccaccg gaatgcatgc acgattcgct cattctcaga caccctgaaa ccatcgcgga
    13861 ttcgatccaa atccagcgtg attgtcgtac ctgtgtcttc agcaacccgc gcgaggatcg
    13921 cctccatttc cttcctgacg tcctggaaat cgtggtcgtc aaaataccga cgtgttccct
    13981 gaatcgttgc ttccaccggg aaacgattgt aaaaatcccc gccatggagt tgtccaaaga
    14041 agatcgtctc cgaacctacg tacggacgca tggtgcgctg caaacgcaca cgttccgctt
    14101 ccagaaccct caggacctca gcggcagcca cgagcggatg cggcgtattc ggcggcgttt
    14161 cgttttcgtg cccgcttttg cctgtcgttt tgaccgtaat ggtgaaaatg gccatcccac
    14221 ggcctgtgat tgccagttca tctaccggtc cttccgccac aacagcggcc gcgccatgca
    14281 gaccttcact gataagcgcg agcagccctt ctccgtggcc ggttggtgcc tcgtgaagcc
    14341 cgtgtgtcgt gagcaggagg tcaccgggta actggactcg cacctcgcgg agaacacgcg
    14401 cggcctccat cacagcagcc agcccaccct tcatatctga cgcgcctcgt cccgaaagca
    14461 cgttgtcaac cagcgaggcg ggggcgtgtg ctgccggaat ggtgtcaata tgtccgtcta
    14521 tctggagtgt gggtgcggca cccgtgccgc gcagtctcgc tgccacgctg ggcgcatccg
    14581 ttccggaggg agaccctttg atcccgtcaa tgcgcgtgat ttcgcagcca agctcgcggt
    14641 acgctgtttc gaagtatcga gtcgcttcgc gagtgaaacc ggtggggctc ggtatggaga
    14701 ccagttccaa cgccgtctgc ccgattcgag cttcgttgac ggcgtcacac aatcgttggt
    14761 gcaacgacat aggatcgttt ccccgtagag tggtataatt cctaacgctg cgtcgaggct
    14821 tttggagcct ggatacgtaa cctaacgccg cgcgtccaga ccgcgcaacc acggagttct
    14881 tcgaaagagg tcgtccatga gcgaatcgtc tcttatgcgg gctgctcgag tggcagtcgg
    14941 gcaatgcctg ggggtccaga agtcggaaca ggttatcgtc atcgcggatg cgccctgtct
    15001 atcgataggt cgtgccttgt tcgaagcctc tgtggagacc ggagcggaca ctcagttgat
    15061 tgaaattctt ccgcgggcgg aaaacggatc tgaaccgccg cccccggtgg cggcagcaat
    15121 ggccgccgcg cacgtggtgt ttgcccctac ctcgaagtcg ctcacgcata cagaagcccg
    15181 gcgcgcggct tcggaggcgg gagcccgcat agcaacgctt cccggcatca cggaagatat
    15241 catgtctcgc gcgcttcttg cagattacga ggcaatcgcc gaacggacgc aacgcgtggc
    15301 gattgcgtta cagggaaaaa agacaatccg tgtcacgact gctgcgggca ctgatatttc
    15361 gttctcgatc gaaggtcgga aggtctcggc cgatacaggc atcctgagaa acccgggggt
    15421 taccaacctg ccggccggtg aggcatatct cgcaccggtt gaaggaaccg gccatggcct
    15481 gatcgtgatt gatggttgca tcggcagcag cggcgtgctg agagaaccaa tcgaggttgt
    15541 tgtcgagggg ggtatggcaa cgattattcg gggcaatgtg tatgcggaaa cgctcgtcgc
    15601 acggatggat gccgcaggac cggaagcaag aaacctcgct gagctgggaa ttggaacgaa
    15661 tgatgcggcg cgattgaccg ggcaggttct ggaggatgag aagatactgg gcaccgtgca
    15721 cctggcattt ggagacaatc gttcaatggg aggtatcgtg cgtgccccat tccatcagga
    15781 cggtattctt ttgcgaccca ctgtatgggc cgatgggtgt ctgattctcg atgacggcgt
    15841 tccaatggtg tgaggccgaa gggagtccga aaatgacgga gaaccgggtg atggaagcgt
    15901 tcctggtggt ctggaaatat cctcttttca ccctcggggg aggcacactt acgctggctg
    15961 tgctcacgaa aactatcctc ggtcttgtgt tcgtctgggt tgtcgctcga gtgatccggt
    16021 ggaaagtgac ggcgggaacg tttgcgaaag ggaggataga gtcctccacg cgatatacga
    16081 tctctcgaat cctgtactac actgttcttt tctttggagt tgtgatcgtt ctggacacgg
    16141 cgggtctgca cctttcctcc cttacggttt tcagtggcct gatgggtgcc ggaatcggtt
    16201 tcggcatgca gaacattgcg tccaatttca tcagcggcat catcctgctc gtggagcggc
    16261 ccgtgcgctc gggagatgtg atatcaattg gcgatacgcg cggagtcgtt gattccataa
    16321 gtatccgcat gacccgggtt gtttcgttcg acaacacgat tcttctcatt ccgaacagcc
    16381 gattggtgaa tgagatgctg gtcagttgga caaccccctc ttaccagttc cgttcttcac
    16441 tcaacgtcgt tgtgcctaac aaacacaatc cggcgcgcgt gaaagagttg ctgcttgagg
    16501 tggcgggcag ggatagccga atacttcctg acccggcacc tgcggtgcga ttcaaggaca
    16561 tgtcggcgac aacccagacg tacgaactgg tagtgtggac caggcaatcg agttctgcat
    16621 ctgaagttcc cagcgacctg cgttttgcga ttgtcgcgca tttcgaggaa aacggggtct
    16681 ctcttgtgac agggtagctc gaactgcaac gtgccgcgga attccactaa attcgggggt
    16741 aacctcagaa agaggggtac ttacgatgcc tgcctacacg acgaaaaacc tgcctctgac
    16801 ctatctgatc aactaccagg atcaccttgg cgacatcgag gcgtcggttg ccccgctccg
    16861 ggacgcaccc ccgaacgtat tccatgcgtg caacgacatg ccctatctct cccgggccgg
    16921 cgcctcgagt taccacgaca gaaagggacc tctggcgtgg tcattcccaa tcgggattga
    16981 ggaggtccgc gaacggacga aagcgactgt caaatgggtg aatgccgttc acgctgccgg
    17041 tgtcgatgtt gtcattccgt acatctgcaa tcagaccata ggcggcgacc ctgagaaacg
    17101 cctcggtttc tggtggtttt ttgaccgctg ggatgattac gccgaggatg ttggccccaa
    17161 accggggatc gatccgatag aatggatgca aagggaagag gatgggagcc ttcacttcaa
    17221 ttacccctac aggttcacgc gcaccgaccc gcctttgcgt tttgccccgt gcccgaacaa
    17281 tccgttctgg cacagctggc tcaaaacgat ggtaaggctc atcgcatcgt acggctacga
    17341 cggaacgttt gtcgacaaca acatcatgca ctgccgttgc aagtattgcc agcaggagtt
    17401 cgcggtattc ctttcggaaa cgtatacaaa aaaagagctt aaggaccgtt atgggtcgac
    17461 cgatccgtct cgtcccaaac ttgccaccgc cggagacgtt atctactggg cgtgccgtca
    17521 acccgaatat gtcaggcagt tccgcgacaa ggaaccggag ttgttcaaag agatattcgg
    17581 gaccacagac gtccgcaaag caaagatgag tgagggaggg acgggcctcc actggatgaa
    17641 atcatacaag ttctggttgg caacgcttga gcgcaccctt ggtgaggaag gtgccgaaca
    17701 tgcgcggcgt gtcggtgatg tctccatgct gggagttcgc aacccccgca tgttatgccg
    17761 gtgggctgat acgcagaaat tctgggcttg gacgatcgcc cgacggaacg cggaactccg
    17821 agaggccgcg cacgaggtga caccgcgctt tctcatcgtc ccgaactggg gtaacatgac
    17881 aggtttctgc cacgctttga gccgttgccg ggacgccaaa aacgtacgtc tgtgggcgcg
    17941 cggcacggat ctgatcttct ttgaggatga attctttccc ggaacgctgg cccccgggta
    18001 taccctcgac ctcatgatct cctacaagta cgcggcagcg tgcggaattc gttcgtgcgt
    18061 acttccgtat cagggagcgg agagccgtaa cctgactgag cttgcgacgg ctgaagcggt
    18121 ggcgttttcc ggagacggcg cgttcgttca gatgccgtac cacttctccg aaatccggca
    18181 ggcctaccgg gcgtttttcg aggggaatcc gcatttgttc cgtggacgtt cctcgtacgc
    18241 gaacgtgggg ctgcttttga gctttgatga agtgcatatg gagaacacac accatctgcg
    18301 cgaatcagcg gcgtgtgcac gatat
//
LOCUS       DAOSOO010000046        17475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3272_length_17475_cov_5.699656, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000046 DAOSOO010000000
VERSION     DAOSOO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17475
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3272_length_17475_cov_5.699656"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..166)
                     /gene="ffs"
                     /locus_tag="PLT86_05145"
     ncRNA           complement(67..166)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLT86_05145"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(262..338)
                     /locus_tag="PLT86_05150"
     tRNA            complement(262..338)
                     /locus_tag="PLT86_05150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:Ala,seq:cgc)
     gene            complement(439..1623)
                     /locus_tag="PLT86_05155"
     CDS             complement(439..1623)
                     /locus_tag="PLT86_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT36031.1"
                     /translation="MARCLPAGGENLFQRIKRVRNEYEKRTGKEAINLSVGEPDGIPT
                     VATRAAAATACMSTDRRMHLYQDNGAPEGFARGLVEYHVGPEIAGRPDLATLPIPGIK
                     PMIGLLPLACGANAGKTVVVAGTTRPGYPVIRTWSGYLGCAYHDWPLYAANGFRPELN
                     DAPAGVTLAVFNYPNNPTGATMSVDKWRELCAWAETHGVRLANDAAYGGLVHGSHAHL
                     SQVATEFPKLEWIELFSASKSHNATGWRVGVAFGTADFVADLTTIKGNTDSGFVAPMA
                     TGVMRAIEVDRAGLDTIRALYGKRITILVDLLSPHLELAAAPEAGFFTFWRIPREAFG
                     HPVRTADEFNALMIENCGIVGVPYTGSDGEYIRYAVCFPVEREDVQAAIRSALASAAL
                     KR"
     gene            complement(1773..2870)
                     /locus_tag="PLT86_05160"
     CDS             complement(1773..2870)
                     /locus_tag="PLT86_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOT36032.1"
                     /translation="MSFEALSACVLGLIALAVGARFLVDGSSRLASALGISPLLIGLT
                     VVAFGTSAPELAVSVHAAWAGQPDIAVGNAVGSNIINVLLILGASAVITPLVVSQQLV
                     RMDTPLMIGACVLVYVFAADGLLSRVNGLILFACVVAYTVFAIRVSKRESAGVEQEYA
                     REYGKTKVTGARTWVVMLAAIVAGLGLLVLGSRFLVEGASEIARAYGFSELVIGLTIV
                     AGGTSLPEIATSIAASARREPDIAVGNVVGSNLFNMLAVLGLSSVFSPAGIPVSRAAI
                     AFDIPVMIGVCVACLPIFFTGYRIARWEGALLLGYWFAYTAYTVFEARGHEASSHLTV
                     VMMEFVIPITLITLATITVRHMREKNRTVPQ"
     gene            3380..5581
                     /locus_tag="PLT86_05165"
     CDS             3380..5581
                     /locus_tag="PLT86_05165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT36033.1"
                     /translation="MKTLPGDEIRQVQWRFSDRFDLQMLVQSARSVARGLVARLVASG
                     ARNTHEWTEEKAQMLTAFDEAGITSVFMDPEDGGYIEGPKNFALALLAFELAWVDGGA
                     ATGSLAGNLALEPIKEKGTEQQRSHYMKLCVPPAPGDTRKAWRGAFALTEPIPYVGVD
                     TGVLGGKVGIARWNDGEEPILHVEKRGRFITNMGFANFVCAAVDSDDPRIKGSCMVIL
                     EEGDPGVFDRGTPTKKLVHQLSSTTDPVFSLDIPASRIIGGYSVKDGVIVPQWNHSQI
                     IESVFRRTRVTVGLMTSAKLLSAIEPVIRYQRGRFRGGASGAEGTPRHDLGLQMKEDP
                     LHRLIDVWATGEASASLGFATARLFDEFDPLERETERLLGDLNVAPGRAQMRTMMEAQ
                     KRAIQFLEESAKPESGRYSTGVASLPDDTLVRFAITDAITNVLCPATKLWNTGHGANM
                     MREAVSLMGGYGITEDCPGFLGMKWMDAQLEATYEGPEAVQRRQLSVTMANELFLTIF
                     RGWIREMRGIASEYPETGACTLASAMELWLWSLTYLLEGKDADGRPLFKDQRQGVTFP
                     MADALSWLLASRCQILDTIELAARGAERGLDAEALPGYVRFFSDLCHVQAARAAGETS
                     RVCSLLVYGYNRHPGWKDEGACLTDEELVSAESLIPGSAAGVAGTADSIGADGTRATK
                     AGPCAGHEGLETFQRLQKRLHACLSGSALAKDRAARALTSVSIPEALDYPA"
     gene            5629..7509
                     /locus_tag="PLT86_05170"
     CDS             5629..7509
                     /locus_tag="PLT86_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOT36034.1"
                     /translation="MGPDLEKGTENPVDRLNLDVDIACVGFGPAMGGFLTTLAREMEQ
                     ASGEPHVQSTVLPGMPLQVVCYERADDVNFGVSGLVTEARAIRNTFPDMNPSEIPLAH
                     PVSEERIVYLLDPHGASRRSLGLRIADSAIRGLRWVLPYRRSALELPFIPPFLKKHGG
                     LVMSIGQFMQWVSNQVSSSGMVQIWPGTPVGEPILEGKQVVGVRIADQGVQKDGTPDV
                     GYMPGMDVRARLTVVGDGPVGAVGQRLDEQFGLPEGHHQHDWAVGMKMVVNLPDHCDL
                     VPGTVLHTLGYPEPEIFGFLYVHPERIASLGIFVPSTLASPVRTVYRYLQHWMLHPYL
                     WHHLEGGTLRSWGAKTLQESGRLGEPYLAGDGYARIGEGSGSTNVLTGSGVDEAWMTG
                     VLLAEGVIDLLKKGEPFTQANLDAACVNRRRKSWVETGSRKAQHARDGFRKGFVTGVL
                     GMAVSGLTRGALNLKTRSAHPREGIPDLNQYYRDRISSEELQRIREECAKNAIPLHDA
                     LMTRAGWPEIPLDGKLLISQQDALLLGGKVQAAPGFADHVMFVDPAVCEQCGTRLCIE
                     ACSAQAIAPSPSGGTPEFDREKCIHCGACIWNCTQPRPGDRERGNIRFYAGAGGLHSA
                     EN"
     gene            7534..8343
                     /locus_tag="PLT86_05175"
     CDS             7534..8343
                     /locus_tag="PLT86_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOT36035.1"
                     /translation="MESGYHIVVCGSLVPDPLQTLEPDVGPKGPALKNEMVLPSVLDP
                     WAAHALYEAAHLAKEYAGSKVWLVSVGPKQRLQQLMMTVAQKAPFSFVPVDGSASGFC
                     DPFETATALANAIGAIPDLDRSRLLLFGGYASASRDAGVTLQVVGEMLGITEVFLAVD
                     SLSVSENGEISLLERVEGGNYLFSECDCPPLVVGWATGHLPEPPNNPQIGMANMRSIM
                     PALQKAPQVQVGAEGVSFAKVELPQQQRTTRIVKDVPVEQIAGEIVAWIRG"
     gene            8370..9413
                     /locus_tag="PLT86_05180"
     CDS             8370..9413
                     /locus_tag="PLT86_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOT36036.1"
                     /translation="MKKILLLAFVEENGALGKPALEALSGACELAQAFGAALNVGLIG
                     EFAQKAADSIASCGANQILAVQGPEFASARCATDSAAAVALIRAAEADFVLAPQTTRS
                     ARCMPGAAFRAKGRIDTRITGLRVDEGTLLIQRWYYRQRMVAELTRSQRPWVVLLEPG
                     VFSPWSGGGGHAEVSIVPVEPVETRTRIAGIEPASAGTQTIRPDADLLFVAGAGWTKK
                     QTDGEVHVEEAENLIRSFLDLTHASLGSSKSLVDQSGEGQKVISLLTHMHQVGQTGST
                     PRHPKGLATCCHGEEPHVVGWRFINERRAVNLDPNCGWARGKADVLYVADAFAVMGKV
                     IELLGKDAGQAAV"
     gene            complement(9450..16808)
                     /locus_tag="PLT86_05185"
     CDS             complement(9450..16808)
                     /locus_tag="PLT86_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT36037.1"
                     /translation="MFSRLRQRVYLLVAAMCVAATSVYAVTPPGTLITSQASLTYFDN
                     GGFQFSLLSNTVVTPVLGSVVGTDGTISQTTQIVAGENITVLLTDPDRNYLPNAIDTV
                     HVTVTNPRTGEVETLVLYETGQGTGVFSGSLPTYYGQSAGPDHDGTLFAQPGDILQTS
                     YNDTATSTGGTAVVTGQTTVLPTWIKLTPEPRTIVANGTDQSRLTARVTDDLDRPLPD
                     GTIVVFTADKGKFSNDTQRIEVPVSGGNGEAVTILTAPILAANDTARVVASFRGFDSD
                     VIKLIILPGAVAVRVYDQTRNVEVRANDPALRVEVELVGTTVTGDPISIFVELDENGV
                     FVVPDIPPGTYQLHVHVTDRVTGADVMVGVLQTIVVNMDGSTSPPRNAIAGMVHARNE
                     ESGARYAGLTVELLDANGNVVATAILDAMGRYDFQNLLPGSYFLRVKMEDGDVFTVPV
                     SSRTNNLGEVVLNADVLIDPFGRVFDAVTTALIPGSTVTLRNLDERVLAIPLLNGTGV
                     PPNVNNINPFVTPADGRYAFLFSSSQVGTVANPARYIMTVDPPSGTTYPPRRMHLTVR
                     PTAEGASTIMMTVSSGDGLQIARPNSFGLTNGPVTVPDIETVAYNIPMFPKTPVLKFS
                     KLASTDTTSYGETVDFAIVVVNVGNDVAPSVTVTDTLNSGWTLVSSDATTSNGGVLTW
                     NVGDLQPGASDTLHVRAEAADQGVILTNAAWAQFTGGLPVSSQADVVSRSASSISITK
                     AVVTPPALVGKEIHYEIRLKTPPGYAGKITVEDPLPAELVYLAGSSVPAATYDAGTHT
                     LTWQVSGATSDTVRTFTFATEPRGDLTPGDYPIPNTANASVGAFKIASNRADDLVTVP
                     YFRITKTSDITTAEPGDFVVYRIALENLSTADSLSNIVVRDVMPFGFEYVANTARLDG
                     GAAPPDTLRDRNIIWNVAGIGAGKTRLLTYRLVLGIGAGAGDGQNTATATASTSRGAT
                     LSAGPATARVFVQPALFTQDQVVLGRAWVDLNGNKVHDDGEPPVPGVVLLMEDGTRVI
                     ADRMGRFSIPEIRTGDHAIRLLEHNIPDSLEPVILGVRSTNDPWMRFVSVSASGMAKA
                     NFPFRVKPRPPAPPPPPPPPPPPALAPPESLTVAVTVERVIVPDSITIPASETIILES
                     GVTFATGKADLRPEGIAVLNDLLPRIKRAQGRQITVSGHTDPRPIRTRQFPNNMVLSL
                     ARARAVRNWMVQNGVDSTHIAIFGYGPDRPIAPNTTPEGMQKNRRVEINLDYGTEART
                     IPAGTARVRLAIRTSGNAERDSFTISQPLPEDITRPAPDSLRDRIVQHGDSVWAKIAR
                     ERGVFTDLPAPEVSHRTWGSIPLRRTSEPRDGTIRETWDGSIRVPAAVDSSTSLIPQD
                     GAVRTVQAPSVAPAGGGSVSPSVSVPAAAGQPPALPGAGAQPAAASTVADSISPAQAP
                     AVELRRLPTPKERVQRPGQLVWIEYPTADSTVSYRDRLTLTARGQTGMPMTLRLNGRE
                     VSTLSVRGDSRVDFLNIAAPSGPVTLTVTQTFPGGRVFADSIFIHVVGPAARVMLDVS
                     PSTLPADSLSQAEAIAQVLDEWGMPLADGQVVTFEIDNGSIITPDLYPEERGVQVQMR
                     GGYASALILSSAKPGEATLTANASNIVARATLNYTVPHERFVLVGIASGQIGWKTNAA
                     PPAGVNFGKDFNSGAYGKGKAAFFGRGTVRNGYLLTTSFDSDRRFDDQVYRYLTPERA
                     YPIHGDASSIFYEAPSASKFFFRLAKERNYLQYGDFAPDLSQAELTAYRRTFTGVAAA
                     MRNRHTAWSLFGAFTEQAIQVDEIPGEGVSGNYYLSAARRGVRVVEGSERIVIQTRDR
                     IHPENILREEVQYRFVDYEIDYLAGTILFKKPIPSRGFDENPIFIVATYEASRALQRH
                     TVGGGRFALHSGDRWEVGTSLVGEGRSTGDYWLSGIDAGYRPFQNVFVSAELARSDLA
                     QTGWAWKFGAQGRYRSTLNYDLSYRSAERAFYNPSSTSALPGVTKMRGKVTWSPVAFA
                     SLTGEAFRSDDVVNREVRNSAALGGTYRWRRLTGLASTEITNVDRAGQETRTGVVSTG
                     LEWAASRTLTFKAERDQNFLDEDVTYRPTLNRLQARWSPLARLDVIAEHAFRDYNFVD
                     SSYTAIGLQSRVTDNTTAYANYKLDGGINGQKNEAIVGLRHRFMPFPYVSMSTGFERM
                     QTLRGNRSADFYSYSIAGEYLPPELFKASARFEQREGQTLDKMVVSGAFDVVLARNLS
                     LLAKHTYAGEATFATGTWNRLRSHHFLSGLAWRSDRSDYLNVLGKYEYKKQYNGMLTP
                     ATDRSVHIGSLEGMLEPRSQLEVFGRYAFKVAQLSSEGVSSRSLTDLWMGSIRYELRE
                     TWDVLGEYRLISQHTANDLQHGASAEVGRVLGGNARIALGYNFVGYHDSDFAGLSYWG
                     QGPFMRIQVKFTEREVAGALDGLQTFWRGR"
     gene            complement(16911..>17475)
                     /locus_tag="PLT86_05190"
     CDS             complement(16911..>17475)
                     /locus_tag="PLT86_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36038.1"
                     /translation="FTNQIWLDANNDGIPGNDGDVLLTDTDGDGKVDTRSIPAGTVAH
                     IIVAVVVTPGTMDMTVDATTVTIASSVDPTVTGTVTLTTTVHAPVLSIVKSVSPTGAQ
                     PPGTVLTYTVVVTNNGHGTATALQIVDMIPTNTTYQAGTIKVNGIARTDAIDGDGARF
                     EGGAIQVRFTAMGPGSSNTFTFDVKID"
BASE COUNT         3424 a         5277 c         5356 g         3418 t
ORIGIN      
        1 ccagaacgcg aaagcgctca tctgccatcg ccggtctcgg tgtgtcgggt gaatgcgggc
       61 ggcggagaag gccagactac ctgcggcgct cgaacaggat cgcctaccgt tgctaccttc
      121 cggtcctggc ggggttcagc ggatgtccgc cgcgcagggc ctggccatcc ccgccgccga
      181 catggagccg atactcgggg actcgataat gtaagcagga ctgagccgtt cgttctgaca
      241 tcagtcctgc agaaaaagaa ttggtggagc agatcgggct cgaaccgacg acctcatccg
      301 tgcgagggat gcgctctccc aaactgagct actgcccctg gactcgcaaa ggtctcgaag
      361 attgctatag caaatataag ccttccacag gcacggtgca acggaagaaa tggcactttc
      421 gtccgccgca cacgtctgtc aacgcttcag tgctgcggaa gccagagctg accgaatcgc
      481 tgcttgaacg tcctcacgtt caacagggaa acacaccgcg taccgaatgt attctccgtc
      541 ggaaccggtg taagggacac ccacgatacc gcagttctcg atcatcaacg cgttgaattc
      601 gtccgcggtg cggacaggat gcccgaacgc ttctcgtgga atgcgccaga acgtgaagaa
      661 cccggcctca ggtgcggcgg cgagttcaag gtgcggcgaa aggaggtcca caaggatggt
      721 gatgcgcttc ccgtacagcg cgcggatggt atccagtccg gcccgatcca cctcaatggc
      781 ccgcatgacg ccggttgcca tcggcgcgac gaagccgctg tccgtattgc ccttgatcgt
      841 cgtaaggtcg gcaacgaaat ccgccgttcc gaacgcgaca cccacgcgcc atccggtcgc
      901 gttatggctt ttcgacgcac tgaacagctc aatccactcc agtttcggga attctgtggc
      961 aacctgcgac agatgagcat ggctgccgtg gacaagccct ccataggccg catcgttggc
     1021 aaggcgcact ccgtgtgtct ccgcccaggc gcacaactca cgccatttgt caaccgacat
     1081 cgtcgcgccg gtcgggttgt tcgggtaatt gaacactgca agcgtcactc ccgcaggcgc
     1141 gtcattgagt tccggtcgaa agccatttgc cgcgtagagt ggccagtcgt ggtacgcaca
     1201 gccaagatac ccgctccacg tgcgaatcac aggatatcct ggccttgtcg ttccggccac
     1261 cacaacggtt ttgcctgcat tggcgccgca tgcgagaggc aacaggccga tcatgggttt
     1321 gatgccgggg ataggaaggg ttgccaggtc cggccttccc gcgatctccg gccccacgtg
     1381 gtattccacg agtccacggg caaagccctc cggcgcgcca ttgtcctggt agaggtgcat
     1441 gcgccgatcc gtagacatgc acgccgtagc cgcagcggcg cgcgttgcca ccgtcggtat
     1501 cccgtccggt tcgccaacgc tgagattgat ggcctctttt ccagttcttt tttcgtactc
     1561 attccgcacg cgcttgatgc gttgaaacaa gttttcacca cctgcgggca gacatcttgc
     1621 cattcagaaa cctcgtgttg tgagaatcag aaacgggcga gttccaattg taaattacta
     1681 ccccgtaaag gcgttacatt tgcagatttc cacttccccg tttcggggcc cccagtttcg
     1741 ggacaccccg ttccggggca ccccgcgccc ggtcattgag gaaccgtacg gttcttttca
     1801 cgcatgtgcc ttaccgtaat cgtcgcgagc gtgatcagtg taataggtat gacaaattcc
     1861 atcatcacga cggtcaagtg gctcgatgct tcgtgcccac gggcttcaaa cacggtgtag
     1921 gccgtgtagg cgaaccagta ccccaggagt agagctcctt cccaccgcgc gatccgataa
     1981 cctgtgaaaa agatcggaag acaggccacg cacacgccaa tcataaccgg tatgtcgaag
     2041 gcaattgcag cgcgcgagac gggaattcct gccggtgaaa agacgcttga cagcccgagc
     2101 accgccagca tgttgaacag attgcttccg accacgttgc ccacagcaat atccggttct
     2161 ctccgcgcac tcgcggcaat ggaagttgcg atctcaggga gcgacgtgcc gcccgcgaca
     2221 attgtcagtc cgatgacaag ctcgctgaac ccgtacgcac gcgcgatctc tgaggcacct
     2281 tccacaagga atctggaacc gagcacaagc aaaccaagtc ccgcaacaat ggctgccaac
     2341 atcacgaccc aggtccgggc acccgtaacc tttgttttgc cgtattcgcg cgcgtattcc
     2401 tgctccaccc cggcagattc tcttttgctc actcgtatcg cgaaaacggt gtaggcgaca
     2461 acacacgcga aaaggatgag tccattcact cggctcaaaa gaccatctgc cgcgaacaca
     2521 taaacgagaa cgcacgcccc gatcatgagc ggcgtgtcca tccggacgag ttgctgcgac
     2581 accaccaggg gagtaataac ggccgaggcg ccgagaataa gcagtacgtt gatgatgttg
     2641 cttcccactg cgttaccgac ggcgatgtct ggttgtcccg cccatgcggc gtgaacgctc
     2701 accgcgagct cgggcgcact ggtgccgaac gccaccacgg taagcccgat caacagagga
     2761 gaaatgccca gtgccgaagc gagtctggag gaaccatcaa caaggaatcg agcgccgacg
     2821 gccagcgcta tcagcccgag aacgcaagcc gacaatgcct caaatgacat gcggtatccg
     2881 aaatgtgaac aggaagcccc gttcgtgaat tgtcagacca agcctgttgt gttcgtgttt
     2941 tcgcacgcga tgggtcgcag ggttctccgt tgtgtggtac gtcgccgtaa gaacaaagat
     3001 tcccgtggga agcaggcgcg taatgcaagt cgtaccataa accgtgacaa cacggtcctg
     3061 ctgcaatcag attggacctg ggccacgcat cgcgaaacgt gctgcaggtc gtatctgctc
     3121 gcccttgact gctccttcgc ggattatgac cttaataaac gtcctgggcg tgattgcagt
     3181 cggcgcaatt caaaacatac gcacctgatt cacccgcgcg caacgttgcg cgcgggccgc
     3241 agggcttcca tccgccctcc caatcggtcg caggaataca catcagcgaa taagtcaggg
     3301 ttccccggcc ttttcctccg ttgatcacgc actacagggg ttccagaaga tgctttcctc
     3361 tgccaatggc gactccgtta tgaaaacact tcccggcgac gaaatccgtc aggtgcaatg
     3421 gcggttttct gaccggttcg acctccagat gctcgttcaa tcggcccgtt ccgtggctcg
     3481 aggtctcgtg gcgcggctcg tggcctcagg ggcgcgtaac acgcacgaat ggacagaaga
     3541 aaaggctcag atgctcacgg ccttcgacga agccgggatc acttccgtct tcatggatcc
     3601 ggaggatggc ggatacatcg aaggaccgaa aaacttcgct ctggccctgc tggcattcga
     3661 actggcgtgg gtcgacggag gggcggccac cgggagcctc gccgggaatc tggcgctgga
     3721 gccgataaag gaaaagggaa cggaacaaca gagatcgcat tatatgaagc tgtgcgttcc
     3781 tcctgccccc ggtgatacgc gaaaagcgtg gcgcggcgca tttgcgttga ccgagccaat
     3841 tccgtacgtc ggcgtagata ccggagtgct gggcggaaag gttggaatcg ccaggtggaa
     3901 tgatggagag gagccgattc tccatgtcga gaaacgaggg cgcttcatca ccaacatggg
     3961 ttttgccaac ttcgtgtgtg cggcggtcga ttctgacgac ccgcgcataa aaggctcctg
     4021 catggttatt ctcgaagaag gtgacccggg cgttttcgac agaggtaccc cgacaaagaa
     4081 gctggtgcac cagctttcgt ctaccaccga ccccgtcttc agtctcgata tccccgccag
     4141 caggatcatc gggggatact ccgtgaaaga cggcgtgatc gttccccagt ggaatcacag
     4201 ccagatcatc gaatcagtgt tccggcgtac acgcgtgaca gtgggcctga tgacctccgc
     4261 gaagctgctt tccgcgatcg agccggtcat acggtaccaa cgtggtcgct tccgcggagg
     4321 agcttcgggg gccgaaggga ctccgcgaca cgatctcggc ctgcagatga aagaagaccc
     4381 gctccacagg cttatcgatg tctgggcgac gggggaagca agcgcctcgc tggggtttgc
     4441 aaccgcgcga cttttcgacg aattcgaccc gctggaacgg gaaactgagc gtctgctggg
     4501 cgatttgaac gtcgccccgg gcagagccca gatgcggacc atgatggagg cgcagaaacg
     4561 agccattcag ttcttggagg agtcggcaaa acccgaatct ggccggtatt ccaccggggt
     4621 agcttctctc ccggatgaca cactggtccg gttcgccatt acggacgcca tcacgaacgt
     4681 gctgtgcccc gcaacaaagc tgtggaacac cggccacggt gcgaacatga tgagagaagc
     4741 agtcagcctg atgggcgggt acggcatcac ggaagactgt cccgggtttc tcggcatgaa
     4801 gtggatggat gcacagctcg aggcaacgta cgaaggcccg gaggcggttc agcgacgcca
     4861 gttgagcgtg accatggcca acgagctgtt tcttaccatt tttcgcgggt ggatacgcga
     4921 aatgcgcggt atcgcctccg aataccccga aacaggggcg tgcaccctgg cgtcagcaat
     4981 ggaactgtgg ctctggtcgc tgacctatct gttggaaggg aaagacgccg acgggaggcc
     5041 tttgttcaag gatcagcggc aaggcgtcac gttccccatg gcggatgcgc tgagctggct
     5101 tctggcgagc cgttgccaga tactcgacac catcgagctg gccgccagag gggcagaacg
     5161 tggactggac gcggaggcat tacccgggta cgtccgattt ttcagcgacc tctgccacgt
     5221 tcaggctgcc cgggcggcgg gcgagacatc gcgcgtatgt tccctgctgg tgtatgggta
     5281 caaccggcat ccaggttgga aagatgaggg agcatgcctg accgatgagg aactcgtaag
     5341 cgccgagtct ctcattccgg gttctgcggc cggggtcgcc ggaactgcgg actcaatcgg
     5401 tgctgacggt acgcgtgcaa ccaaagcggg gccgtgtgcg ggtcatgagg ggcttgagac
     5461 gtttcagcgg ctgcagaagc gcctgcatgc ctgcctttct ggttccgcgc tcgccaagga
     5521 cagggcggcc agggcgctca cgagcgtttc catccccgaa gcgctcgact accctgcctg
     5581 aacagtggtg ttgtggcgcg gatttgaggg cgattggaag aaaacggaat ggggcccgat
     5641 ttggaaaaag ggacagaaaa cccggtcgac cgtttgaatc ttgacgtgga tatcgcgtgc
     5701 gtagggttcg gccctgccat gggcgggttt ttgaccaccc tcgctcgcga gatggaacag
     5761 gcatcgggtg aaccgcacgt tcagagcacg gttctgcccg gaatgccgct tcaggtagtc
     5821 tgctacgagc gtgccgacga cgtcaatttc ggtgtgtccg gactggtgac ggaagccaga
     5881 gccattagaa acacattccc cgacatgaat ccctccgaga ttccgttggc tcatccggtc
     5941 tccgaggaaa gaatcgtgta tctactcgac ccgcatggcg cgagcaggcg gtcgctgggt
     6001 ttgagaattg ccgacagcgc aattcggggt ctccggtggg tgctgcccta tcgccgctcg
     6061 gcattggaat tgcctttcat tccgccgttt ctcaagaaac atggcggcct ggtcatgtcg
     6121 atcgggcaat tcatgcagtg ggtgagtaac caggtctcgt cctcaggaat ggtccagata
     6181 tggccgggaa cacccgttgg agagccgatc ctcgaaggga aacaggttgt cggtgtgcgt
     6241 attgcggatc agggcgttca gaaggatggc acgcctgacg tcggctacat gccgggaatg
     6301 gatgttcgtg cgcgcctcac cgtcgtcggc gacggtccgg tcggtgcggt ggggcaacgt
     6361 cttgacgagc agttcgggct cccggaaggc caccatcagc acgattgggc ggtcggcatg
     6421 aagatggtcg tgaacttgcc cgaccattgt gacctcgtcc cgggaacggt cttgcacacg
     6481 ctgggatacc cggaaccgga gatcttcggt tttctttacg ttcacccgga acgcatcgcg
     6541 tcgctcggca ttttcgttcc ctctactctc gcgagtcccg tccggacggt ataccgctat
     6601 ctgcagcact ggatgctcca cccttacctc tggcatcacc tcgaaggcgg cacgttgagg
     6661 tcatggggcg cgaaaacgct tcaggaatct ggtcgcctcg gagagccgta cctggcaggc
     6721 gacggatacg cccgaatcgg agagggatcc ggcagtacga acgttcttac gggatccggt
     6781 gtggatgagg catggatgac cggtgtcctg cttgccgaag gcgtcatcga tctgctgaaa
     6841 aagggggaac cctttactca ggcgaacctc gacgctgcat gtgtcaaccg gcggaggaaa
     6901 agctgggtgg aaacaggctc gcgcaaagcc caacacgcca gagacggctt ccggaagggc
     6961 ttcgtgaccg gtgtcctcgg catggctgtt tcgggtctta cgcgcggcgc gctgaacctg
     7021 aagacccgat cggcacatcc tcgcgaaggc ataccagacc tgaaccagta ttacagggac
     7081 cggatttcga gtgaagagct tcagcgaata cgcgaagagt gcgcgaaaaa cgccattccg
     7141 ctgcatgacg cgctgatgac aagggcgggg tggcctgaaa ttcctctgga cggcaaactg
     7201 cttatttctc agcaggatgc gcttctcctc ggcggcaaag ttcaggcagc gccggggttt
     7261 gctgaccacg tcatgtttgt cgatccggca gtgtgtgaac agtgcgggac caggctgtgc
     7321 atcgaagcct gttctgcgca ggcaattgct ccgtcacctt caggcggaac tccggagttt
     7381 gatcgcgaga aatgcatcca ctgtggcgcg tgcatctgga actgcacgca gcctcggccg
     7441 ggagacaggg aacgcggcaa tatccggttt tatgccgggg caggcggcct gcattcagcg
     7501 gaaaattgac tttttcagaa ggaacaggag ttcatggaat ctggatatca catcgttgta
     7561 tgcggaagtc tggtgccgga tccgttgcag accctcgagc cagatgtcgg accgaaagga
     7621 cccgcgctta agaacgagat ggtcctgcca tccgtgctgg atccgtgggc ggcccacgcg
     7681 ctgtacgagg cggcgcatct cgcgaaagaa tacgcgggat cgaaagtgtg gctggtcagt
     7741 gttggtccga agcagcgact gcagcagctc atgatgactg tcgcgcagaa agctccgttc
     7801 agcttcgtgc cggttgatgg gtctgccagc ggcttctgcg acccgtttga aaccgcaacc
     7861 gcgcttgcga atgcaatagg tgcgatcccc gacctggaca ggagccggct gcttctcttc
     7921 ggagggtacg cgtctgcgtc gcgggacgcg ggcgtaaccc ttcaggttgt aggcgaaatg
     7981 ctcgggatta cggaggtctt tctcgcggtt gattcgctgt cggtttctga gaacggcgaa
     8041 atcagtcttc tcgaacgcgt ggaaggcggc aattacctct tcagcgagtg cgattgtccc
     8101 ccgctcgttg tcggctgggc gacaggccac ctgcctgagc cgccgaacaa cccgcaaatc
     8161 gggatggcga acatgcgaag catcatgcct gctctgcaga aggcgccgca ggtgcaggtt
     8221 ggtgcggaag gggtatcgtt tgcgaaggtg gaactcccgc agcagcaacg aaccacgcgc
     8281 atcgtcaagg atgtgccggt ggaacagatt gccggggaaa ttgtcgcgtg gatacgggga
     8341 tgactgagcc agtgaggaac gatagcgcaa tgaaaaagat ccttcttctc gcatttgttg
     8401 aggaaaatgg ggcgctcggg aaacctgcgc ttgaagcgtt atccggcgcg tgcgagcttg
     8461 cacaggcatt cggtgcggca ctgaacgtcg gattgattgg cgagtttgct cagaaagccg
     8521 cggacagcat cgcttcgtgt ggcgcaaacc aaattctcgc cgtccaggga ccggagttcg
     8581 cgtctgcaag gtgtgcaacc gattcagcgg cggctgtggc cttgattcgc gctgccgaag
     8641 cggatttcgt cctggcgccg caaacaaccc gttcggcgcg ttgcatgcct ggtgctgcgt
     8701 ttcgggcgaa gggccgcatc gacacgcgca taaccgggct ccgcgtggac gaaggtacac
     8761 tcctcatcca gcgctggtac tatcgccaga ggatggtcgc tgagctgaca cggtctcagc
     8821 gaccgtgggt ggtgctgctc gagccggggg tgttcagccc ttggagcggg ggcggtgggc
     8881 acgcggaggt ctccatcgta cctgtggaac cggtggaaac acgtacccgg atcgccggca
     8941 tcgaaccggc atccgcggga acccagacga ttcgccccga tgcggacctc ctgttcgttg
     9001 caggggccgg ttggacgaaa aagcagaccg acggggaagt tcatgtcgaa gaagccgaga
     9061 acctgatccg gagcttcctt gacctgactc acgcatccct cgggagcagc aaatccctgg
     9121 tggatcagtc cggggaaggg cagaaggtga tcagccttct gactcacatg catcaggttg
     9181 gtcaaacggg gtccacgccg cgccatccca aagggcttgc gacctgttgc cacggtgagg
     9241 aaccgcacgt ggtgggatgg cgattcatca acgaacggcg cgccgtgaat ctcgatccca
     9301 attgcgggtg ggcgaggggg aaggcggacg ttctctacgt cgccgacgcg ttcgctgtca
     9361 tggggaaggt gatcgaactg ctggggaagg acgccggaca ggcggcagtc tgacagagcc
     9421 ggcttcggct tcggcttctg aactcaagct cacctgcccc gccagaaggt ctggaggccg
     9481 tccaacgctc cggcgacttc gcgttccgtg aatttcacct ggatccgcat gaaaggcccc
     9541 tgtccccagt atgacaggcc cgcgaagtcg ctgtcgtggt aaccgacgaa gttgtacccg
     9601 agcgctattc gtgcattgcc gccgagaaca cggcccactt ccgccgaagc tccgtgctgc
     9661 agatcattgg ctgtgtgctg gctgatcaaa cgatactccc cgagcacatc ccaggtttcc
     9721 cgcaattcgt agcggatgct tcccatccac aggtctgtga gcgaccgcga ggacactccc
     9781 tcgctggaaa gttgggccac cttgaacgcg taccggccga acacctcaag ctgagagcgc
     9841 ggctccagca taccttccag cgatccgatg tgtactgaac gatccgtggc cggcgtcaac
     9901 atcccgttat actgcttctt gtactcgtat tttccgagga cgttcagata atcgctacgg
     9961 tccgaccgcc atgcgagacc ggacaggaaa tgatggcttc gcaaccggtt ccatgtaccc
    10021 gtggcgaatg tcgcttcgcc cgcatacgtg tgtttcgcaa gcagggaaag gttgcgtgcc
    10081 agcacaacgt cgaacgcccc cgaaaccacc attttgtcga gcgtctgacc ttcccgctgt
    10141 tcgaagcggg cgctggcctt gaataattcc ggaggcaggt actccccggc gatgctgtac
    10201 gagtagaaat cggcggagcg attgcctctc agcgtctgca tccgctcgaa gcctgtgctc
    10261 atggatacgt acgggaacgg catgaaccgg tgccgcaaac cgacgattgc ttcgttcttc
    10321 tgaccgttga tcccgccgtc cagtttgtaa ttggcgtatg ccgtcgtgtt gtccgttaca
    10381 cgggattgca ggccgatggc ggtgtagctg gaatccacga aattgtagtc gcggaacgca
    10441 tgctccgcta tcacgtccag ccgcgcaaga ggtgaccagc gcgcctgaag gcggttcagc
    10501 gttggacggt acgtaacgtc ttcgtcaagg aagttctgat cccgttccgc cttgaaagtg
    10561 agtgttcgcg acgccgccca ttcgagtccg gtgctgacca ccccggtgcg ggtttcctga
    10621 cctgctctgt caacgttcgt gatttccgtg gacgcaagcc ccgtgagtct gcgccagcgg
    10681 tacgtgccac ccagtgcggc gctgttccga acctcgcgat tcacgacgtc gtcggaacgg
    10741 aacgcttccc ccgtaagact ggcgaaagca accggcgacc acgtaacctt tccgcgcatc
    10801 ttcgtcacac cgggaagggc ggaagtgctg ctcggattat agaacgcacg ctcggcgctt
    10861 ctgtaggaaa ggtcataatt cagagtgctt cgataccgtc cctgcgcgcc gaatttccat
    10921 gcccatcccg tctgcgccag atccgacctt gccagttccg cgctgacgaa cacgttctgg
    10981 aatggccggt aacctgcgtc aatgccgctc aaccagtaat cgcccgtgga gcgtccttcg
    11041 cccacaagcg aagtgccgac ttcccatctg tcgccggaat gaagcgcgaa acgaccgcca
    11101 ccaaccgtgt gccgctgaag cgcgcgtgac gcttcgtagg tggcgacgat gaaaatcggg
    11161 ttctcgtcga aaccccggct cgggattggc tttttgaaca gaatcgtacc ggcgaggtag
    11221 tctatctcat agtcgacgaa gcgatactgc acttcttcgc gcaggatgtt ttccgggtgg
    11281 atgcggtcgc gcgtctgaat gacgatgcgc tcgcttcctt ccaccacgcg aacgccacga
    11341 cgcgccgcgg acaggtaata gttccccgaa acgccttcac cagggatttc gtcaacctgt
    11401 atcgcctgtt ccgtgaacgc accaaacaga ctccacgcgg tgtgtctgtt gcgcatcgcc
    11461 gcggccacac ctgtgaacgt ccgcctgtat gccgtaagtt ctgcctgcga cagatcggga
    11521 gcaaaatcac cgtactgcag gtagttccgt tcctttgcga gccggaagaa gaacttgctc
    11581 gcggaaggcg cttcgtagaa aatggaactg gcgtcgccat ggatgggata ggctcgttct
    11641 ggcgtgagat aacgatagac ctggtcatca aaccggcggt cgctgtcgaa cgaggtcgtc
    11701 aggaggtacc catttcggac cgtcccgcgg ccaaagaatg cggccttgcc ttttccatac
    11761 gcgccgctgt tgaagtcctt tccgaaattc acaccggcag gcggagcggc gttcgtcttc
    11821 catccaatct ggcctgatgc tataccgacc agcacaaatc tttcgtgcgg cacggtgtag
    11881 ttgagagtcg cccgcgcgac gatgttcgac gcgttcgccg tcagtgttgc ctcgcccggt
    11941 ttcgccgaac tcaggatcaa tgcagaagcg tacccgccac gcatctgaac ctgcactccg
    12001 cgttcctcgg ggtagaggtc cggcgtgatg atcgaaccgt tgtcgatctc aaaggtgacg
    12061 acctgtccat ccgccagcgg cataccccac tcgtccagaa cctgcgcgat ggcttccgcc
    12121 tgggagaggc tgtcggcggg aagcgtggac ggggagacgt caagcattac cctggcagcc
    12181 gggccgacca cgtggatgaa gatgctgtcg gcgaacacgc gaccgcccgg gaacgtctga
    12241 gtgacggtga gggtcaccgg tcctgacggc gcggcgatgt tcaggaaatc tacccgcgaa
    12301 tcgccacgga ccgagagagt agacacttcc cggccgttca gccgcagggt catcggcatg
    12361 ccagtctgcc cgcgcgctgt cagcgtcagc cgatcccgat aactcaccgt gctgtccgcc
    12421 gtcggatact ctatccatac aagttgaccg ggtcgctgca cgcgctcctt cggtgtgggc
    12481 agccgtcgaa gttcgactgc gggcgcctga gccggcgaaa tgctgtctgc aacggtgctc
    12541 gcggccgctg gctgcgcacc tgcgccggga agtgcgggtg gctgtcccgc cgccgccgga
    12601 accgaaaccg acggggaaac agaaccacca cctgcaggag caacggaagg cgcctgcacc
    12661 gttcgaaccg cgccgtcctg tggaatcagc gaagtgctgg aatcaactgc cgccggaacc
    12721 cggatacttc cgtcccatgt ctcgcgaatc gttccatccc ttggttcgct cgtgcgccgc
    12781 aacgggatcg aaccccacgt gcggtggctc acttcaggcg cgggcaaatc cgtgaacacg
    12841 ccgcgttccc gtgcgatctt ggcccacact gagtccccgt gctgcacgat gcgatctcgc
    12901 aacgaatccg gtgcaggacg cgtaatgtcc tccgggagcg gctgcgagat cgtgaaacta
    12961 tcgcgttccg catttccaga cgtgcggatc gcgagcctca cgcgggcggt tccggcaggg
    13021 atcgttcgcg cctcggtccc gtaatcgagg ttgatttcaa cgcggcggtt cttctgcatc
    13081 ccttcgggcg tggtgttggg cgctattggg cggtcgggac cgtacccgaa tatcgcgatg
    13141 tgcgtcgaat caacgccgtt ctgcaccatc cagttgcgca cggcacgcgc acgcgcaagc
    13201 gacaaaacca tgttgttcgg gaactgccgt gtgcggatcg gccgcgggtc ggtgtgcccg
    13261 ctcaccgtga tctgccggcc ctgcgcccgt tttatcctcg ggagcaggtc gttcagcacc
    13321 gcgattcctt cgggccgcag atccgctttc ccggtggcga acgtcacacc ggactccaga
    13381 atgatggttt ccgacgccgg gatcgtgatc gaatctggaa cgatcacgcg ctccacggtg
    13441 accgccacgg tgagcgactc cggcggggcc agtgcgggtg gcggaggagg cggtggtggt
    13501 ggcggtggcg ctggcggacg cggtttcacg cggaacggga agttcgcctt cgccatcccc
    13561 gaggcggaca cgcttacgaa ccgcatccac ggatcgttgg tcgagcggac gcccagaatc
    13621 accggctcga ggctgtcggg gatattgtgt tccagaagcc gtatcgcgtg gtcgccggtg
    13681 cggatttccg gaatcgagaa gcggcccatc cggtcagcga tgacgcgcgt gccgtcttcc
    13741 atcaggagaa ccacacccgg caccggcggc tcgccgtcat cgtgcacttt gttgccgttc
    13801 aggtccaccc acgcgcgacc gagtaccacc tgatcctgcg tgaacagtgc cggttgaacg
    13861 aacacacgcg ccgttgccgg tcctgcggaa agcgtagctc cgcgcgacgt ggaagcggtt
    13921 gccgtggcgg tgttctgtcc gtcgcccgcg ccggcgccga ttcccagcac gaggcggtac
    13981 gtcagcaggc gtgttttccc ggcgccgatg ccggcaacgt tccagatgat gttccgatcg
    14041 cgcagcgtgt cgggtggcgc ggcaccaccg tccagccgcg cggtgttcgc cacatactcg
    14101 aaaccgaacg gcatcacgtc ccgcaccacg atgttcgaca ggctgtcggc agtgctcagg
    14161 ttctccagcg cgattcggta gaccacgaaa tcgcccggct ccgcggtggt gatgtcggat
    14221 gtcttcgtga tacggaaata cggcaccgtc accaggtcat ccgccctgtt cgaagcgatc
    14281 ttgaacgccc cgacgctcgc gttcgcggtg ttggggatcg ggtaatcgcc cggcgtcaga
    14341 tcgccacgtg gttccgtcgc gaaggtgaac gtccgcacgg tgtcactcgt cgcgccggag
    14401 acctgccacg tcaacgtatg tgtgccagca tcgtacgtgg cggcgggaac gctgcttccc
    14461 gcgagataca caagctccgc cggcagcgga tcttctacgg tgatcttccc ggcgtacccc
    14521 ggcggcgtct tcaggcggat ttcgtaatgg atttctttcc cgacaagcgc cggcggcgtc
    14581 acaactgcct tcgtgatgga aatcgaggat gcgctgcgcg aaaccacatc cgcctgcgag
    14641 gacaccggca gcccgcccgt gaactgcgcc cacgcggcgt ttgtcagaat cacaccctga
    14701 tccgccgcct cagcgcggac gtggagcgta tcgctggcgc cgggttgcag gtcgccgaca
    14761 ttccaggtca ggacgccgcc attggacgtc gtcgcatcgg aagaaaccag cgtccacccg
    14821 ctgttcaggg tgtcggtcac cgtcacggac ggcgcgacat cgttccccac gttgacgacc
    14881 acgatggcga agtccaccgt ttcaccgtag ctcgtcgtat cggtggaggc gagcttcgaa
    14941 aacttcagca ccggcgtctt cgggaacatc ggaatgttgt acgcaaccgt ctcgatatca
    15001 ggaaccgtga ccggaccgtt ggtgagcccg aacgagttcg gccgcgcgat ctgcagcccg
    15061 tccccggagg acaccgtcat catgatggtc gacgcgcctt ccgccgtcgg tcgaacggtg
    15121 aggtgcatcc tgcgcggcgg atacgtcgtc ccggacggcg ggtcaaccgt catgatgtac
    15181 cgcgccgggt tcgccaccgt gccgacctgc gacgaactga acaggaacgc ataccggcca
    15241 tccgcagggg ttacgaacgg gttgatgttg ttcacgttcg gcggcacgcc ggtcccgttg
    15301 agaagcggaa tcgccagcac gcgttcatca aggttccgca gcgttactgt gctgcccggg
    15361 atgagcgccg tggtgacggc atcgaacacc cgcccgaacg ggtcaatcag cacatcggcg
    15421 ttcagcacca cttcgccaag attattcgtg cgcgacgaca ccggcaccgt aaagacgtcc
    15481 ccgtcttcca tcttcacgcg caggaagtag ctccccggca gaaggttctg gaaatcgtac
    15541 ctgcccatgg cgtcgagaat cgccgtcgcc accacgttgc cattggcatc cagcagctca
    15601 accgtcaggc cggcataccg cgcgcccgat tcctcattgc gcgcgtgaac catgcccgcg
    15661 atggcgttcc gtggcggtga cgtggacccg tccatgttaa cgacgatcgt ctgcagaacg
    15721 cccaccatca cgtccgcgcc ggtcacccgg tcggtcacgt gcacgtggag ctgatacgtc
    15781 cccggcggaa tgtcaggaac cacgaacaca ccgttttcgt cgagttcaac gaatatcgag
    15841 atggggtcgc ctgttaccgt ggttcccaca agctccacct caacgcggag cgccgggtca
    15901 ttcgcccgca cctccacatt ccgggtctgg tcgtacacac gcaccgcaac ggcgcccggc
    15961 aggatgatga gcttgatgac gtccgaatca aatccgcgga acgacgccac cacccgcgcg
    16021 gtgtcgttcg ccgccagaat cggggcggta aggatcgtca ccgcttcccc gttgccgccc
    16081 gataccggca cttcgatccg ttgcgtgtcg ttcgagaact tgcccttgtc cgccgtgaag
    16141 acgacaatcg ttccgtccgg cagcggccgg tcgaggtcat cggttacgcg ggcagtgagc
    16201 ctcgattgat ccgttccgtt cgccacgatg gtgcgcggct ccggcgtaag tttgatccac
    16261 gtggggagaa ccgttgtctg acccgtcacc acggccgtgc cgccggttga ggtcgcggtg
    16321 tcgttgtaag aggtctgaag tatgtccccc ggttgcgcaa acagcgtccc gtcatggtca
    16381 gggcctgcgc tctgaccgta ataggtgggc agggaaccgg aaaacacgcc ggttccctgc
    16441 ccggtttcgt acaacaccag ggtctcaacc tcgcccgtgc gcgggttcgt cacggtcacg
    16501 tgcaccgtgt caatcgcgtt ggggaggtag ttccggtcgg ggtcggtaag aagaaccgta
    16561 atattctcac cggcgacgat ctgtgtggtc tgggaaatcg taccgtccgt tccgacgaca
    16621 ctcccgagca ccggcgtaac aacagtgtta ctcaggaggg agaactggaa gccgccgttg
    16681 tcgaaatacg tgagggaggc ctgactggtg atcagtgttc ccggcggcgt aacggcgtac
    16741 acactggtcg ctgccacgca catggcggcg accagtaggt acacccgttg ccgaagacgc
    16801 gagaacatct atcagtcccg attctgcttt ttgtcgtcag tgcgcccctc tcccgcccga
    16861 aggacgggag aggggcatga acatgctcag ttcaattcac gcgtcgtgcg tcagtcaatc
    16921 ttgacgtcga acgtgaaggt gttgctgctg cccgggccca tcgcggtgaa gcgaacctgg
    16981 atggcgccgc cctcgaatct cgcgccgtca ccgtcaatcg cgtcggtgcg tgcgattccg
    17041 ttcactttga tcgttccggc ctgatacgtc gtgttggtcg gaatcatgtc cacgatctgc
    17101 agggcggttg ccgtgccgtg accgttgttc gtcacgacga ccgtgtacgt gagcacggtc
    17161 cccggaggct gcgctcccgt cggggaaacc gacttgacga tggatagaac gggcgcatga
    17221 acggtcgtgg tcagtgtcac cgtaccggtc actgtcggat caacactcga tgcaatcgtt
    17281 acggtggttg catctaccgt catgtccatc gtccccggag tcaccaccac cgccacgatg
    17341 atgtgggcaa cggtgccggc cgggatgctt cttgtatcca ccttgccgtc gccgtcggta
    17401 tccgtcagca gaacgtcgcc gtcatttccg gggatgccgt cgttgttggc atccagccag
    17461 atctggttgg tgaag
//
LOCUS       DAOSOO010000047        17256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3326_length_17256_cov_5.774257, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000047 DAOSOO010000000
VERSION     DAOSOO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3326_length_17256_cov_5.774257"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLT86_05195"
     CDS             <1..967
                     /locus_tag="PLT86_05195"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT36039.1"
                     /translation="LGVEGYWIAALVTDVILVVPMVLEGSRKFLRGRPVWRFLREMLG
                     IGFPMTFAALGGIAVNMSGQYALNRFAGTELVGIYAAATRIASMFAMLLSYPIAAWWG
                     PHVLSEAEKCGPGRLRELLANYLRVLIAVGVVLLGLISLGGALIVWFPIHESYRSAVP
                     LVGLCILPLLIQVLSYPFTTQFIYAKRTHYFASGGVLTGAVAIVLNALVTPYWGIGGV
                     ILANVAAACAGTMLYYAGGRREFPVAYDWKPVWTNGALVMGYVACAVAAMSPGASIST
                     HVVLFAALMLSLGALAANRRVWHMFSWRISNFISERTRNGGESWF"
     gene            958..2658
                     /locus_tag="PLT86_05200"
     CDS             958..2658
                     /locus_tag="PLT86_05200"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT36040.1"
                     /translation="MVLSSHGRTNYVIVLSSQASRSEMHAATELQRFLEEMTGAHFPL
                     LDDGVPVAEKEIAVGAGRHTEALLPGVDFGAFGSEELLVKTVGERIVIAGGRQRGTLY
                     GVYRFLEILGCRWFTAKVSRIPRVETLTVEPLDTREKPLLEYREPFFAEAFDGDWAAR
                     NLANSNSASLMARHGGKVRYGTFVHTFETLLPVAKYFDEHPEWYSEIDGKRISERPQL
                     CLTNPQVLEKVIEGVRGWIKAMPDATIFSVSQNDWTNPCQCAKCREIDEREGTPQGSI
                     LQFVNKVAEAIEDEAPHVAIDTLAYWYSRKPPKTLKPRQNVIIRLCSIECCFAHPLES
                     ECPENQKFAEDIREWSRIANRLYVWDYVTNFHNYIMPWPNFKVLGANVRFFARHNVVG
                     LFEQGSYAPGGGGEAAEMRAYVLAKLLWNPSVDENRIRDEFIDGVYGKAAGKVREYVR
                     VIHEPVADPNIHMHIFCDITNPHLTDDVIERGDALLYEAEALAESEAVRERVELAHLP
                     MHYVRIRKMTDVPEKAEERKALLERFLAIARREGIRNISEQHTIETWAQHGAIEPGKW
                     "
     gene            2783..3769
                     /locus_tag="PLT86_05205"
     CDS             2783..3769
                     /locus_tag="PLT86_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36041.1"
                     /translation="MSLKIGFIGAGGIAGAHVAGLRKMADVQLVAFSDVQEERAARLV
                     ADTGAKVFTDWLKMLDTEQMDAVYICVPPHVHGAPELACIERKIPFFVEKPISNDLDT
                     ATNILGKVRRAKVMTAVGYMNRYRRSLQRGKELLAGARISTLEGHWLGGTPGAPWWYR
                     KAQSGGQLVEQTTHIVDAIVYLAGRVTEVYAVGAKGTHADPPEGYDVEDATAVTLKFA
                     SGAVGSINSACSLKAGGGVGLDVFSPSVVLSYRDWNMSLKATKSRLETEDVRGEDNIF
                     EIEDRAFVEAVMKKDPALIRCNYGQAFHAHHVTMAANRSLETGMPVQLADFV"
     gene            3906..4112
                     /locus_tag="PLT86_05210"
     CDS             3906..4112
                     /locus_tag="PLT86_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOT36042.1"
                     /translation="MATYTINIEGMTCQHCVKAVTTALSNVAGVERVTVSLQKKQATL
                     EVDEAKFKPEAARKAVINEGYVPR"
     gene            complement(4253..5869)
                     /gene="groL"
                     /locus_tag="PLT86_05215"
     CDS             complement(4253..5869)
                     /gene="groL"
                     /locus_tag="PLT86_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT36043.1"
                     /translation="MPKQLEFEVDARESLRKGLNTLADAVKVTLGPKGRNVVIDKKFG
                     APTVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFNHG
                     LKNVTAGANPMDLKRGIDAAVTAAVKAIKEQSRPISGKDDMAKVAAISANNDEEIGKL
                     IADAMEKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYQSPYFVTNPDTMETELDEPL
                     ILIYDKKISAINDLLPVLQKVAQIQKPLLIISEEMEGEALATIVVNKLRGTLKAAAVK
                     APGFGDRRKAMLEDIATLTGGRVISEEAGFKLENVVLSDLGRARRVIIDKDNTTIVEG
                     AGSREAISGRIKQIRRQIEETTSDYDREKLQERLAKLAGGVAVVNVGAATEVEMKEKK
                     ARVEDALHATRAAAEEGVVPGGGVALVRAASALDTLELTGDQKIGADIVRRALDEPLR
                     WIALNAGQEGSIVVQKVKEGKGAFGFNAQTEVYEDLYKSGVIDPTKVVRTALENAASV
                     AGLMLTTEATITDIPEEEKAPAVPPGGGMY"
     gene            complement(5884..6174)
                     /locus_tag="PLT86_05220"
     CDS             complement(5884..6174)
                     /locus_tag="PLT86_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT36044.1"
                     /translation="MNIKPLGDRVLVKPIEESEQVRGGIIIPDTAKEKPQQGEVKAVG
                     PGKRGEDGTVWPMNVAVGQIVLYGKYAGTEVKIDGEDYLIMSEGDIYGIVEK"
     gene            6779..10090
                     /locus_tag="PLT86_05225"
     CDS             6779..10090
                     /locus_tag="PLT86_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT36045.1"
                     /translation="MRQRFKWHIVLMALLVTLGTLPTTSWAALTGKIAGRVSDQSGNP
                     LPGVAVFIQGRQLGATTDSDGRYFILQVPPGTYTVEASLVGYQSVRLQNVTVNMDRTT
                     EANFSLRESTIEVDAVTVVAQRPIVQQDVTSTQLSVTTEQAQQLPVNTLLDAIGFQAG
                     IDIQNKTTISVRGSSPDQINFQVDGFQQTNPIENRSYTTMNQALIQEVQVLTGAFTAE
                     YFSRAAVVNVVTRDPGEKPNFSGDFRYIPARKQHFGVGAYDANQFDRLLYRSPAGTDA
                     GGRLFAKDANGNWTKIIDSDKPIWLPRTDLTDISPSADAASSVQIFQGWDALLTSLNS
                     AVPAAQANDRTLTVTKGKWTKAALIEYWDWQHRGVDYANQGDYYIDAALTAPLVVLPR
                     TGVVIGFKDLRSQLGVPAIQQAYTDRRIDLGFKSSLLPTIKLELRGSYQVTRATMYGN
                     DAVGGNVGTQDMQLFFSSEGASNGGNSLYNFLCNRLQDVSEGGAFNKYNLASNVPHKE
                     TTWGLGARVTHTLSRSTYYSLSYEYFTGKIDAKHVAHRDTTRNIALVNKDSAGNTIYV
                     NEGPMGFWPSEGTTGYGYTDVPGVYLLTGGGYASDFSKYNYSRLRFDAFSQVNRNNGI
                     KLGAEAILNNLTKDYRRVTRDLGRLGHWVQYETSPWQLGAYVQDKMEYEGLIANVGMR
                     IDGFNSGADILWPDLLAPAVFQRAQLIPWLEAQGMTVLPDDWLRNVRMANVPMAQDSG
                     WMYAQIADGYFPKKAATTKWKLSPRLGISHPVGTSTKFFFNFGWMYSTPKAGWLYGYS
                     PESFQNGSRGSEIRGMSNPNLDMARSVVYEVGFEQSIRNTYVARVKGYSKDDDSMPVA
                     FQGVGWDTLAKTGNTLDLVHNGAFSTSRGMEISLDKVAGRFFGASLQLDYRVYSSGNE
                     GFQTIYDTTSFGAVNGNKGKINVPYRVTSGQSEVQPSLNANIVLRTPSDWGMMRGDWS
                     LSLNQTWSRGGTMNYDPFNTGNPTKLRFVDVYRTNVRLNKGLSMGSRNVSFYVDVRNV
                     FNQKTLNIAALRNQSDYIALLATMNPSTGKFDQKYKVGDSRLEDVVERRFARDNDWLL
                     YLYPREFQVGMRVNL"
     gene            10146..12641
                     /locus_tag="PLT86_05230"
     CDS             10146..12641
                     /locus_tag="PLT86_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36046.1"
                     /translation="MERTRYLRLQGWAGLGAMVGALLGLAGATTTSAAPSSPNLPNYL
                     PSSWTVSGQPMKQPGEAIDRATRGVLQHARYGRFWQPQLDNMWRVGYNGTAVWPGGRV
                     MRTGGRDNREGWGSYGTAWGGAVGCRDWDATLPTSPTDPTPVTATYPYCSNENLRYGY
                     NLDDVMLDKLCVSNSNGAANPIVGYSRLETAEISRWLPPQVIVNGKVVIPYTDTYGTT
                     PGSPAAGNGLANYAAARRGVVDPTLRAEVVQLTAFRMAIGVDIYRTTYNYTCPPDWDY
                     LVQDFAVINTGNLDRDAAIDRPAKAINDFRFATGFEHTTSGVANGMINPNRNKDDDFI
                     ELIYPWASDATYGTRSAYLAYDGDGSGIDGPDWGDPAQTVSGTTQTMGKDLFSKAYMA
                     FAYLFAESSPGSGVDDPTQPASFTYFEENGYRLGRTRPGWDQGKAFKMIWCGHENAAT
                     NAPTKVIPPNTPTNGTAVNATVLAVFQGLVGPQPAGWTLPAGGTYRVVAGIAAGGISD
                     RLSKDLAFATLTRDAAGQPAASRQTASEIALIQSGRDSALRTLDRMFWNVNGFVPGGR
                     YASKPPAYNQAFNVPDPPRPPACFWVSNGDRKIDLEWTREAETTPDFDTGVNDFAGYR
                     VYRAASSPDSDWVLVKDLPKASLPTTGTTVKWSDAAPSVSRDFTYFYYVVAYDDGTQN
                     WDTTPGIAKSLESGKYYTTTGWDWSPVNPGFFASPSTYPMTHLDSVIVVPNPYNSRSY
                     NPKGAQGTIDRIFFKNLPQAATIRIYTAAGSPVKTLYHFAAPGAADGASIGWDMSNDV
                     NQVVATGVYIYTVECTAGPAQGRTAVGKFIIIR"
     gene            12666..13694
                     /locus_tag="PLT86_05235"
     CDS             12666..13694
                     /locus_tag="PLT86_05235"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT36047.1"
                     /translation="MKRTLIVGAMTCLLGLAVVNPTFAAQRGQATMTWLGISASARDE
                     AMGGTATAGDASAACAFTNPAGLGRVERGSVFFNTTSWLADMSVNDVAFAYRVPDIGT
                     FSLAFRSMDYGDFTFTTLFANEDGYVYTPSDSTGDVAGMMLGIGYGRKLTDKFSIGGQ
                     LKYVSDKLGKMNVLDRGLNIVNFGEEANQSAMLFDFGTSYHTGWKSVTINMTIQNFGR
                     SQKASNGVEEFTPPLTFKVGLQADALQFAGLETPMADLLVRVEGADPRDDRLGFNTGA
                     ELTLKPMDMVNVALRGGYSRRDQGGLTFGVGVGTNVGGFNTQLDWAYSDWGGVLGATN
                     RLGVSFAF"
     gene            13915..17010
                     /locus_tag="PLT86_05240"
     CDS             13915..17010
                     /locus_tag="PLT86_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36048.1"
                     /translation="MRRVHFLWIGVALLAAALLVTLYETHEVVLPNSTCVPCHGVTIF
                     TEEIMDESRGAFTGPDACEPCHAYAYHRWTKSDHCRAMMGPAPSTIAGHFSADTVEYS
                     YRDFHSAMILRDGKYFMIAPNKKGQPEEFPIHLALGIRDQQALLTKFPDGRYQILPTA
                     YDIRNQTWFESSEGIVRTTHMLRPSEPFYWTNWKRTWNYDCFGCHLSGMEKNYDPETN
                     TYNTKWRDLGIDCEACHGPGRAHARLRTVKKLWGGLVRDTTMLRLTSLSPRQQVETCG
                     ACHASKSTLALGYKPGDDWTEYYTLTLLDHDFIMPDGRYAGLIYDLIGFMQSPCYEKG
                     GLTCITCHSGHADERSIELRHYEHQNDMCRPCHTDKVENPQAHTFHPNDSPGSTCRNC
                     HMQVLPVSRMGIVDHTISIPTPENTIAFNSPNACQSENCHPNETPQWSIATLKRWHGP
                     NRIDRWTRAQVLFKGKTLDPAGVPGLIAMLADTGNNMIWRATAARMLGNIGDARAVPV
                     LRANIDAPDPMLRWQVANALGNFVYDRRVFTDLQSLLRRERHSQIRVAVASKLGYWWR
                     RDLNLTPAEKEIARQTWEQYVRQMTTAHADDPIARNTLGMVYMRRGEFDKAIREFQIS
                     LRIEPGSSGVERNLADVYTAMNRYSEALPHNQRAVDLEPDNPHLYDALSFTYLGLGRI
                     ADAEREAQRALRLDPRFAPGYVQMARIRHAQGNIAEAHRLLQKAVALDARNVEAQYLL
                     AKASQELRLRDQAVQAFVNVISLDEFSEFGYDAKNQLATMLPVGAPLPPPTATPAPER
                     PAQEGPMRPIDPHLLWPTVVYKSYAPKTTPEEVARIPEPSSFAASLDSGRAWMRLAEN
                     SRLSSATKKAYIDRSVATLSRAHRMAGSDRSKDVEVRKALGYALSVQAEHVPPANAPA
                     LLARARSVLDGVDTTALGGDAAAKASLGRSYYRLARAYNETRDYRNAIWAFERAAVLV
                     PNSFTEAISHFFVGTSYDRLGQRQEAIAAYQRSASNPHNSGRGLRCSLHGIKYPFRYF
                     R"
BASE COUNT         3618 a         5018 c         5082 g         3538 t
ORIGIN      
        1 tctcggtgtg gaggggtact ggatcgcggc gctagtgact gacgtgatcc tcgtggtgcc
       61 gatggtgctg gaggggagcc gcaaattcct ccgcgggcgc cctgtctggc ggttcctgcg
      121 ggagatgctg ggaatcggat tccccatgac gttcgccgcg ctgggcggga tcgcggtgaa
      181 catgtccggc cagtacgccc tcaaccggtt cgcgggaacc gagcttgtcg gaatctacgc
      241 ggcggccacg cggatcgcat cgatgttcgc catgctgctt tcgtatccga ttgccgcgtg
      301 gtggggcccg catgtgcttt ccgaagcgga gaagtgtggc cccgggcgtc ttcgggagct
      361 tctggcgaat tatctccgcg tgctgattgc ggtgggggtg gtgctgctgg gtctcatttc
      421 gctgggtggt gcgctcatcg tctggttccc gattcacgaa agttaccgga gtgccgttcc
      481 gctggtcggg ctttgcattc tcccgttgct catccaggtt ctgtcctacc ccttcaccac
      541 gcagttcatt tacgcgaaac ggactcacta cttcgcctcg ggcggcgtcc tcacgggtgc
      601 cgtggcaata gtcctgaacg cgttagtgac tccgtactgg ggaataggtg gcgtcattct
      661 tgcaaacgta gccgctgcgt gcgcaggcac gatgctctac tacgcgggcg ggcggaggga
      721 attcccggtc gcgtacgact ggaagcccgt ctggacaaac ggtgcgctcg tgatggggta
      781 cgtggcgtgc gcggtcgcgg caatgtcacc gggtgcctcg atcagcaccc acgtcgtcct
      841 tttcgcggcg ctgatgcttt cgctgggcgc tctcgcggca aaccgtcgag tatggcacat
      901 gttttcgtgg agaatttcca atttcatctc ggaaagaact cgcaatggag gtgaatcatg
      961 gttctgagtt cacacgggcg cacgaactac gtcatcgtcc tgtcgtccca ggcatcgcgc
     1021 tcggagatgc acgccgcaac cgagctgcag cgcttcctcg aggaaatgac gggagcccat
     1081 ttccccttgc tcgatgacgg ggtgcctgtc gcggagaagg aaatcgcggt cggcgcaggc
     1141 cgtcacaccg aggccctgct tcccggcgtc gacttcggcg cattcgggtc tgaggagctg
     1201 ctggtcaaaa cggtgggcga gcgcatcgtc atagcaggag ggcgtcaacg ggggaccctg
     1261 tacggggtgt accggttcct tgagatactg gggtgccgtt ggttcaccgc aaaagtctcc
     1321 cgaatcccgc gcgtggagac cctgacggtg gaacctctgg acacaaggga gaagccactt
     1381 ttggagtacc gggagccgtt tttcgcagag gcgttcgacg gcgattgggc ggcgcgaaac
     1441 ctcgcgaatt ccaacagcgc cagcctcatg gcccggcacg gcgggaaggt cagatacggc
     1501 acattcgtgc acacgttcga aacgctcttg cccgtcgcga agtactttga cgagcatccc
     1561 gagtggtaca gcgaaattga cggcaaacgt atctctgagc ggccgcagct ctgtctgacg
     1621 aatccgcagg tgctggaaaa agtgattgag ggggtgcgcg ggtggatcaa agcgatgccc
     1681 gacgccacca ttttcagcgt aagccagaat gactggacga atccctgcca gtgcgcgaaa
     1741 tgccgcgaaa tcgacgaacg ggaaggtaca ccgcagggaa gtattctcca gtttgtgaac
     1801 aaggttgccg aggcgattga agacgaggcg ccgcatgtgg caattgatac cctcgcgtac
     1861 tggtattccc gcaagccacc gaagacgttg aagccccgcc aaaacgtcat aattcgtctc
     1921 tgctcaatcg agtgctgctt tgcccaccca ctggaatccg agtgcccgga gaaccagaaa
     1981 ttcgcggagg atatccgcga atggagcaga atcgcgaacc ggttgtatgt gtgggattac
     2041 gtcacgaact tccacaacta cataatgccg tggccgaatt tcaaggttct gggcgcgaac
     2101 gtccgtttct tcgcccgcca caacgtggtc ggcctgttcg aacagggcag ctacgcaccg
     2161 ggcggcggcg gcgaggcggc ggagatgcgc gcctacgtgc tggccaaact gctctggaac
     2221 cccagcgtgg atgagaaccg gatccgggac gagttcatcg atggcgtgta cggaaaagcc
     2281 gctggcaagg ttcgggagta tgtcagggta atccatgaac cagttgccga cccgaacatt
     2341 cacatgcata tcttctgcga catcacaaat ccgcacctta ccgacgacgt gatcgagcgc
     2401 ggcgacgcac tgctgtacga ggctgaagcg cttgcggaat cagaagctgt gcgagaacgc
     2461 gttgaattgg cgcatctgcc catgcattat gttcgaatcc gaaaaatgac cgatgttccg
     2521 gagaaagccg aggaacgcaa agccctgttg gagagattcc tcgccatcgc cagacgggag
     2581 ggcatccgga acatctcgga acagcacacg atagaaacct gggcgcagca cggggcgatc
     2641 gaaccgggca aatggtaatc tggcaacgtt ccaccgcaag caccctttct cccggcggag
     2701 aaagggtgct ttcttttgag tggaatcgga gaaggtagtt ctctttcaca aagacccccg
     2761 cagcgtaagg aaaggactcg aaatgtcgct gaagatcggt ttcatcggcg ccggaggcat
     2821 tgccggagca cacgtggctg ggctcaggaa aatggcggac gtccagcttg tggcgttttc
     2881 tgacgtccag gaggaacgtg ccgctcgcct tgtggcggac accggagcaa aggtgttcac
     2941 cgactggctg aagatgctcg acaccgagca gatggatgcg gtctatattt gtgtgcctcc
     3001 gcacgtacac ggcgcgccgg aactcgcctg catcgaacgc aaaataccct tcttcgtgga
     3061 aaaaccaatc agcaacgatc tcgacacggc aaccaacatc ctcggcaaag tgcgccgcgc
     3121 gaaagtgatg acggcggtcg ggtacatgaa tcgttaccgg cgcagcctcc agagaggaaa
     3181 agaactgctc gcgggagcca ggatttcgac tctggaaggc cattggctgg ggggaacacc
     3241 gggagcaccg tggtggtacc gcaaggcgca atccggcggt caactcgtgg aacagacgac
     3301 ccacatcgtt gacgccatcg tctatcttgc gggacgcgtg acagaagtgt acgccgtcgg
     3361 ggcaaaaggg actcacgcag acccaccgga agggtatgat gttgaggacg cgactgccgt
     3421 gacgctcaag ttcgcaagcg gcgccgttgg ttccatcaac tcggcatgtt cgctcaaggc
     3481 gggaggaggg gtcggactgg acgtgttcag cccgtcagtg gttctctcat accgggattg
     3541 gaacatgagt ctcaaagcga ctaaatcccg tctggagaca gaggacgtgc gcggcgaaga
     3601 caacatcttc gaaatcgagg atcgggcctt tgtggaggcc gtgatgaaaa aggaccctgc
     3661 actgattcgc tgcaactacg gtcaggcgtt ccacgcccac catgtgacga tggcggcaaa
     3721 ccgctctctg gagacaggaa tgcctgtgca attagccgat ttcgtctagc agatcaaacc
     3781 ggaatctcga tgaagcgggt gcggtgccag ccgccccttt ccttttgtca tgtgtcccgg
     3841 aacgaagagc cgggccgaca ttgttctatt tgatggacct gcgggatttc ggaaggagat
     3901 tgaaattggc aacgtacacg ataaacatcg agggcatgac gtgtcagcat tgcgtcaaag
     3961 cggtcactac ggctctgtcc aatgtcgccg gcgtcgaacg cgtcacggtg tccctgcaaa
     4021 agaagcaggc aacgcttgaa gtggacgagg cgaaattcaa acctgaagcc gcgcggaagg
     4081 cggtgattaa tgaaggctac gtgcccagat aggtagaagg cctgagaact gacccgccgc
     4141 cagtgaccac cgagttcatc cgtttgcccg gggtgcgagc ggcaagatag aagcagggcg
     4201 cgacctttcg gccgcgccct gcttccgttc gtggaggttc gcagtgatga tctcagtaca
     4261 tcccgccgcc cgggggcacc gcgggagcct tctcttcttc gggaatgtcg gtgattgttg
     4321 cttccgtggt gagcatgagg cccgcaacgc tcgccgcatt ctccagggcg gtgcgcacga
     4381 ccttggtcgg gtcgatcaca ccggacttgt acaggtcctc gtacacctcg gtctgggcat
     4441 tgaagccaaa cgctcctttg ccttccttca ccttctggac tacgattgag ccttcctggc
     4501 ccgcgttcag agcaatccac cggagaggtt cgtccagagc gcgccgaaca atatccgcac
     4561 cgattttctg atcgccggtg agctcgagag tatccagcgc agaagccgct cggacgagcg
     4621 ccacgccacc acccggcaca acaccctctt cagcggctgc tctcgtcgcg tgcagtgcat
     4681 cctcaacgcg cgccttcttc tccttcatct ccacttccgt ggcagcacca acattgacaa
     4741 ccgcaacgcc gccggcgagt ttcgcgagcc tttcctgcag cttttcgcgg tcgtaatcac
     4801 tggtggtttc ctcgatctgt cgccgaatct gcttgatccg gccgctgatg gcctcgcgtg
     4861 atcccgcgcc ttcgacaatc gtcgtgttgt ccttgtcgat gatcacacgg cgcgcacgtc
     4921 cgaggtcgct cagcacgaca ttttcgagtt tgaacccggc ttcctcgctg atcacacgtc
     4981 cgcccgtaag cgtggcgatg tcttccagca tggctttgcg gcggtcacca aatccgggcg
     5041 ccttcaccgc cgccgctttc agcgtgccgc gcagcttgtt cactacgatg gtggccagcg
     5101 cctcgccttc catttcctcg gaaatgatca gcaacggttt ctgaatctga gccaccttct
     5161 ggaggaccgg aagcaggtcg ttgatcgcgg agatcttttt gtcataaatc agaatgagcg
     5221 gttcatccag ttccgtctcc atcgtgtccg gattggtgac aaagtacggt gactggtaac
     5281 cgcggtcgaa ctgcatcccc tcgaccacgt cgagcgtagt ctcgatgctt tttgcttctt
     5341 ctacggtgat aacaccgtct ttcccaactt tttccatcgc gtcggcgatc agcttgccga
     5401 tttcctcgtc gttgttcgcg ctgattgcgg caaccttcgc catgtcgtcc ttgccggaaa
     5461 tcgggcggct ctgttctttg atggccttca ccgctgccgt caccgcagcg tctattccgc
     5521 gcttcagatc catcgggttg gcgcccgcgg taacattttt caggccatgg ttgaaaatcg
     5581 cctgcgcgag aacggttgcc gtagtcgttc cgtcaccggc tacgtcagag gttttggaag
     5641 caacttcctt caccatctgc gcacccatgt tttcgaactt gtcttcgagt tcgatttctt
     5701 tcgcaacggt aacgccgtct ttggtgacgg tcggagcgcc gaatttcttg tcaattacca
     5761 cgttccgccc tttgggaccg agcgtgactt tgaccgcatc ggcgagtgtg ttcaagcctt
     5821 tgcgaaggct ctcacgggcg tctacttcaa attcgagttg ttttggcatg ggaatctcct
     5881 cgattacttc tcaacgatgc cgtagatgtc gccttcggac atgatcaggt aatcttcgcc
     5941 atcaatcttc acttccgtgc ccgcgtattt cccgtagagc acgatctgcc ctaccgcgac
     6001 gttcatgggc cacacagtgc cgtcttcgcc gcgttttccc ggcccgactg ccttgacttc
     6061 gccctgttgc ggtttttcct tcgcagtgtc agggatgatg atccccccgc gcacctgctc
     6121 gctctcttcg attggcttaa ccagaacgcg gtcacccagt ggtttgatgt tcatgtgcga
     6181 tctcctctca tgacgaaaat cacaggagtt cggttagttc gtccgacata ttagcactca
     6241 ggtaacgcga gtgctaacaa cgcttgctaa agtataggac tggcaatcgg gtttgtcaag
     6301 tgaatcatgt cgcttcgtcc acttttttct ctccctgagg agttccaaaa gctgaggggt
     6361 tcggattgta cggccccgct cttacacttt cccaagaatt aaacattaaa aaccccattc
     6421 gcccttgaca cgcgcaggaa tgcacgccat cattattccg attgtgccct ttctaccttg
     6481 ttcggggccg ttcgaggaaa ccagttcaga accgctcgga gtgatcaaaa acatgcgcgt
     6541 ccgcacgatg acattgttcc tgcttcggcg ctccccgcgc cggtgcctga acgaatcaac
     6601 ggcacgcgct gcgcaacccg accagaccac actcctgcca tctttcccga actttcgtcc
     6661 attttcccaa acctgcgctc accacgtctt ttcaagaaag gagtaatgcg aaaggcacga
     6721 gcgtttttgt gtttcaccaa atggttgtgg tcgtcaaagt gagaggagag gagttctcat
     6781 gagacagcgg ttcaaatggc acatcgtgct gatggcgctt ctcgtgacac tggggacgct
     6841 gcccacgact tcctgggcgg ctcttaccgg gaaaatcgca ggtcgagtat ccgaccagtc
     6901 cggtaacccg ctccccggtg tagcggtgtt catccaggga cgccagctcg gcgcaacaac
     6961 ggattcagac ggacggtatt tcattctgca ggtgccgccc ggaacgtaca cggtcgaagc
     7021 gagccttgta gggtaccaga gcgttcgcct gcagaacgtc acggtcaaca tggaccggac
     7081 gacggaggcg aatttctcgc tgagagagtc cacgatcgaa gtggacgcag taaccgtcgt
     7141 ggcacaacgt ccaattgttc aacaggacgt aacgtccacc cagctctccg tcacgacgga
     7201 acaggcgcaa cagcttccgg tcaacacgct tctggatgca attggttttc aagccggtat
     7261 tgacattcag aacaaaacga cgatcagcgt gcgtggaagc tcacccgacc agattaattt
     7321 ccaggtggac ggtttccagc agaccaaccc gattgaaaac cgttcgtaca ccacgatgaa
     7381 ccaggcgctt attcaggaag ttcaggttct gacaggcgca ttcacggcgg aatatttcag
     7441 ccgtgcggcg gttgtcaacg tggtgacacg ggatccgggc gaaaagccca atttctcggg
     7501 cgatttccgt tacatccctg cacggaagca acacttcggt gtgggcgcgt acgacgccaa
     7561 tcagtttgac cgtttgttgt accgcagccc tgccggcacc gatgccggag gaaggttgtt
     7621 tgcgaaagac gccaacggga actggaccaa aatcatcgat tcggacaaac cgatctggct
     7681 tccccgaact gatctcacgg acatttctcc gagtgcggat gccgcctcct ccgtgcagat
     7741 attccaggga tgggacgcac tattgacctc actcaacagc gcagtgcctg ccgctcaggc
     7801 gaatgaccgc acgctgaccg tcaccaaggg caaatggaca aaggcagcgc tcatcgagta
     7861 ctgggactgg cagcaccgcg gcgtggatta cgcaaatcag ggcgattact acatcgacgc
     7921 agccctgacg gccccgcttg tggttctccc ccgcaccggc gtggtcatcg gtttcaagga
     7981 cctccggtct cagctcggtg tgccggccat tcagcaggcc tacactgacc ggcggatcga
     8041 cctgggcttc aaatcgagcc tcctgccgac catcaagctg gaactgcggg gaagctacca
     8101 ggtgacgaga gccacgatgt acggcaacga tgcggtgggc ggtaatgtcg gcacacagga
     8161 catgcagtta ttcttctcgt ctgaaggtgc ttcaaatggc gggaactcac tgtacaactt
     8221 cctgtgcaac cggttgcagg acgtaagcga agggggagca ttcaacaaat acaacctcgc
     8281 gtctaacgtt ccgcacaaag agacaacctg ggggcttggt gcacgggtta cgcacacgct
     8341 ctcccgctcg acgtattact cgctgagtta cgagtacttc acgggcaaaa ttgacgccaa
     8401 acacgtcgct caccgtgata cgacccgcaa tattgcactg gtcaacaagg actcggcggg
     8461 caacacgatc tacgtaaacg aagggccgat gggcttctgg ccgagcgaag gcaccaccgg
     8521 ttatggatac accgacgttc ccggcgtgta tcttctgacc ggcggcggat atgcatctga
     8581 tttctcgaag tacaattaca gccgtttgcg gtttgacgca ttctcacagg tgaaccgcaa
     8641 caacggcatc aagttgggag ccgaggctat cctgaacaat ctgaccaagg attaccgacg
     8701 cgtgacacgt gaccttggcc gcctcgggca ctgggttcaa tacgagacgt caccgtggca
     8761 gctcggcgcg tacgttcagg acaaaatgga gtacgaaggc ctgatcgcga acgttggtat
     8821 gcgcattgac ggcttcaact ccggcgccga tatcctctgg ccggatcttc ttgcgccggc
     8881 ggtgttccag cgcgcgcagt tgattccctg gctggaagcg cagggcatga cggttcttcc
     8941 agacgactgg ctccgcaatg ttcgcatggc gaacgtgccg atggcgcagg actcgggatg
     9001 gatgtacgcg caaatcgcgg acggatattt cccgaagaaa gccgccacca cgaagtggaa
     9061 gctgtcgcca cgtctcggca tcagtcatcc ggtcggcaca tctaccaagt ttttcttcaa
     9121 cttcggctgg atgtacagca ccccgaaagc cggctggttg tacgggtact ctccggaaag
     9181 cttccagaac ggcagccgtg ggtctgaaat ccgcgggatg tcgaatccga atctcgacat
     9241 ggcgcgctca gtcgtgtatg aagttggctt tgaacagtcg attcggaata cgtatgtcgc
     9301 tcgggtcaaa ggctattcga aagacgacga cagcatgccc gtggcgttcc agggcgttgg
     9361 ctgggatacc ctcgcaaaaa ccggcaacac gctcgatctc gtgcataacg gcgcattttc
     9421 tacttcgcgc ggcatggaga tcagcctgga taaggtagcc ggacggttct tcggcgcctc
     9481 tttgcagttg gattaccggg tgtactcgtc cggaaacgag ggcttccaga cgatatacga
     9541 caccacatcg ttcggggcgg tcaacgggaa caagggcaag atcaacgtcc cgtaccgcgt
     9601 cacgtccggg caatccgaag tgcagccgtc gctgaacgcg aatattgtgc tccgtactcc
     9661 gtcggactgg ggtatgatga ggggcgattg gagcctttcg ttgaaccaga cgtggagccg
     9721 cggcggcacg atgaactatg atccgttcaa caccggcaac cccacgaagc ttcggttcgt
     9781 tgacgtgtac cgcaccaacg tgcgcttgaa caagggtctg agcatgggtt cacgcaacgt
     9841 gagcttctac gtggacgtgc gcaacgtgtt caaccagaag acgctcaata ttgccgcact
     9901 gaggaatcag agcgactaca ttgctcttct ggccacgatg aaccccagca ccggcaaatt
     9961 cgatcagaaa tacaaagtcg gtgacagcag gctcgaagat gtggttgaac ggcggtttgc
    10021 gcgagacaat gactggctcc tgtacctgta tccgcgtgag ttccaggttg ggatgcgtgt
    10081 gaatctctaa cgtatagttt acgcgagcgt tttctgcgag gaacacgctc gctgtggagg
    10141 cccaaatgga aagaacacgc tatctgcgtt tgcagggctg ggctggtctg ggagcaatgg
    10201 ttggtgcgct gctgggattg gcaggcgcaa caaccacttc cgcagcgcca tccagcccga
    10261 atttgccgaa ttacctgcca agttcgtgga cggtaagcgg gcaaccaatg aaacaaccgg
    10321 gcgaggcgat tgaccgggcg acccgcggtg ttttgcagca cgcgcggtac ggccgtttct
    10381 ggcagccaca gctcgacaac atgtggcgcg tggggtacaa cggtaccgcc gtctggcccg
    10441 gtggccgggt tatgcgcacc ggaggacgcg acaaccggga aggttggggc tcgtatggca
    10501 ccgcgtgggg cggcgcagta ggatgccgcg attgggatgc aacgcttccc acttctccca
    10561 ccgacccaac tcccgtaaca gcaacgtacc cgtactgctc gaacgaaaat ctgcggtacg
    10621 gatacaatct ggatgacgtg atgctggaca agctttgcgt gtccaacagc aacggcgccg
    10681 ccaatccgat tgtggggtac tcacgattgg aaaccgcgga aatcagccgg tggctgcccc
    10741 cgcaggtcat cgttaacggc aaggtagtga tcccgtacac cgacacgtac ggcaccacgc
    10801 cgggttcccc cgcggcgggg aacggtctgg ccaactacgc ggcggcacgc cgcggggttg
    10861 ttgaccccac cctgcgcgcc gaggtagttc aactcaccgc gttccgcatg gcgatcggtg
    10921 tagatatcta ccgcaccacg tacaactaca cctgcccgcc tgactgggat tacctcgtgc
    10981 aggatttcgc cgttatcaac acagggaacc tggaccgcga tgcggcaatt gaccgcccgg
    11041 ccaaggcgat caacgatttc cgtttcgcaa ccgggtttga gcacacgacc tcgggtgttg
    11101 cgaacgggat gatcaacccg aacaggaaca aggacgatga tttcatcgaa ctgatctacc
    11161 cgtgggcttc tgatgcgacg tacggcacgc gttcggcgta tctcgcgtac gatggcgacg
    11221 gatcgggcat cgacgggcct gactggggtg accccgcgca gaccgtgtcc ggaaccaccc
    11281 agaccatggg caaagatctc ttttccaaag cgtacatggc gtttgcgtac ctgttcgcgg
    11341 aaagctcccc gggatcaggt gttgacgatc cgactcaacc cgcatccttc acatatttcg
    11401 aggagaacgg gtatcgtctg ggtcgtacgc ggcccggttg ggatcagggc aaggccttca
    11461 aaatgatctg gtgcggccat gaaaacgccg caactaacgc cccgaccaaa gtcattccac
    11521 ccaacacccc gaccaacggc actgcggtga acgcgactgt gcttgcggtg tttcaggggc
    11581 tcgttggtcc gcaacctgct ggttggacgc tgccggcagg tggtacgtac cgcgtggttg
    11641 ccggcattgc cgccggtggt atcagcgatc gcctgtcgaa ggaccttgcg ttcgccacat
    11701 tgacgcgtga cgcggcaggc cagccggcag caagtcgcca gacggcatct gaaatcgcgc
    11761 tgattcagag cggacgcgat tcggcgttgc gcacgcttga tcgcatgttc tggaacgtga
    11821 acgggtttgt tccaggcgga agatatgcca gcaaaccgcc agcgtacaat caggcgttca
    11881 atgtgcctga tcctccgcgt ccgccggcat gcttctgggt gtcgaacggc gaccgcaaaa
    11941 tcgacctcga atggacccgc gaagcagaga ccaccccgga tttcgatacc ggtgtgaacg
    12001 acttcgccgg ttaccgggtt tatcgcgcgg cgtcctcacc ggatagcgac tgggtactgg
    12061 taaaggacct gccaaaggcc agtctgccaa caaccgggac gacggtcaaa tggagtgatg
    12121 cggcgccgag tgtgtcgcgc gacttcacgt atttctacta cgtggttgcg tacgacgacg
    12181 gcacccagaa ctgggacacg actcccggaa tcgcgaaaag cctcgagagc ggcaagtact
    12241 acacgacaac cgggtgggac tggagccccg tgaacccggg cttcttcgcg agcccgtcaa
    12301 cgtaccccat gacccatctg gatagtgtga tcgtcgtgcc gaacccttac aactcccgga
    12361 gctacaaccc caagggagcg cagggaacga tagatcgcat tttcttcaag aatctgccgc
    12421 aggcagccac gatccggatt tacaccgcag cgggatcacc cgtgaaaacg ctgtatcact
    12481 ttgcggcacc gggcgcggct gatggcgcga gcatcggctg ggacatgagc aacgatgtca
    12541 accaggtggt cgcaaccggc gtgtacatct acacagttga gtgcaccgcc ggtcccgcgc
    12601 agggtagaac ggcagtgggc aagttcatca ttatccgtta gcccgacagg atggaggaac
    12661 atcagatgaa gagaaccttg attgtcgggg caatgacatg cctgctgggc cttgccgttg
    12721 taaacccgac tttcgcagcg cagagggggc aggccaccat gacgtggctg ggcatctccg
    12781 caagcgcgcg cgatgaggca atgggaggga ctgctaccgc gggagacgcg agcgcagcct
    12841 gtgcgttcac caaccccgcc gggttgggcc gcgttgaacg cggaagcgtt ttcttcaaca
    12901 ccacgtcgtg gcttgccgac atgtcggtga acgatgtggc atttgcctat cgcgtacctg
    12961 atatcggcac gttttccctc gcgttccgtt cgatggacta cggcgatttc acgttcacca
    13021 ccctgttcgc aaacgaggat gggtacgtgt acacaccgag tgactcgacg ggcgatgttg
    13081 ccgggatgat gctgggcatc ggctacggcc ggaagttgac cgacaagttc agcatcggcg
    13141 gccaactgaa gtatgtgtcc gacaaacttg gcaagatgaa cgtgttggac aggggcctca
    13201 acatcgtcaa cttcggtgaa gaggcgaatc agagcgcgat gctgttcgat ttcggaacga
    13261 gctaccacac cggatggaag agcgtcacga tcaacatgac gattcagaac ttcgggcgct
    13321 cgcagaaggc ctcgaacgga gttgaggagt tcacccctcc cctcacgttc aaggtgggtc
    13381 tccaggctga cgcgttgcag ttcgccggcc tggaaacccc gatggcggac ctcctcgttc
    13441 gcgtggaagg tgccgaccct cgtgacgacc gactcggttt caacacggga gctgagctta
    13501 ctctcaagcc tatggacatg gtcaacgtgg cgctgcgggg aggttattcc cgccgcgacc
    13561 aaggcggcct gaccttcggt gtgggagtcg gcacgaacgt tggtgggttc aacacccagc
    13621 ttgactgggc gtactccgac tggggtggcg tcctgggtgc aaccaaccga ttgggagtct
    13681 ccttcgcatt ctgacagagg cgtggaggca atcttgcgtg ggcggagtcg gcagcgactc
    13741 cgcccacgtt tgttgtagac actttccctc ctgaattgcc ttctgtggct ttcgttcggc
    13801 tacccgcgta ttttaagtag ttgtcacctg tgctggtttg agctgagttc gtcgtgtcga
    13861 gccgaccggt cggcgccgca ttgaccttgc gcccgacaac acacgggagt tgttatgcgt
    13921 cgcgttcatt tcctgtggat cggagtagct ctcctcgccg ccgcgcttct cgtgacgctg
    13981 tacgagactc atgaagtcgt gcttcccaat tcgacgtgcg ttccgtgcca cggagtcacc
    14041 atctttacag aagaaatcat ggacgagagc cggggcgcgt tcaccggtcc agacgcgtgc
    14101 gagccgtgcc atgcgtacgc ctaccaccgg tggacaaaat ccgaccattg cagggcgatg
    14161 atggggccgg cacccagcac gattgccggc catttttccg ctgacacggt ggaatactcg
    14221 taccgcgatt ttcactccgc aatgattctg cgcgacggca agtatttcat gatcgcgccg
    14281 aacaagaagg ggcaacctga ggagttcccg atccaccttg ccctgggtat ccgggaccaa
    14341 caggccctct tgacaaagtt tccggacggc cgttaccaga tcctcccgac cgcgtacgat
    14401 atacgtaacc agacgtggtt tgaatcaagc gaaggaatcg ttcgtaccac ccacatgctg
    14461 cgtccgagcg agcccttcta ctggacaaac tggaagcgga cctggaatta cgactgcttc
    14521 ggctgtcatc tcagtggcat ggagaagaat tacgaccccg aaacgaacac gtacaacacc
    14581 aaatggcgag accttggcat tgattgtgaa gcgtgccacg ggccgggccg ggcgcacgca
    14641 aggctgcgga cggtgaagaa gctctggggt ggattggttc gtgacacgac catgctccgc
    14701 ctgacaagtc tttccccgcg acaacaggtt gaaacgtgtg gtgcctgcca cgcaagcaag
    14761 agcacgctgg cgctcgggta caaaccaggg gacgattgga cggaatacta cactctcact
    14821 ctacttgacc acgatttcat catgccggac ggtcgctacg cgggactcat ctacgacctg
    14881 ataggtttca tgcagagccc ctgttacgag aaaggcggtt tgacctgtat tacgtgtcac
    14941 agcggtcacg cggatgaaag atccatcgaa ctccgtcact acgaacatca gaacgatatg
    15001 tgccggccgt gccacacaga caaagtcgag aacccgcagg cccacacgtt ccatccaaac
    15061 gatagccctg gcagtacctg tcgcaactgc cacatgcagg tccttcctgt gagtcggatg
    15121 gggatcgtgg accatacgat ttcgattccc actccggaaa acacgatcgc gttcaactca
    15181 ccgaatgcct gccagagcga gaattgtcat ccgaatgaaa cgccccagtg gtcgatcgca
    15241 actctgaaac ggtggcatgg tccgaacaga attgaccgct ggactcgggc gcaggtgctg
    15301 ttcaaaggga aaactctgga tccagcgggc gtgcctggat tgatcgcgat gcttgcggac
    15361 acgggcaaca acatgatctg gcgcgcgact gcggcacgca tgcttggaaa catcggtgac
    15421 gcgagagccg tgcctgtgtt gcgtgcgaat atcgacgctc ccgacccgat gcttcgctgg
    15481 caggtcgcaa atgcgctcgg caacttcgtg tacgatcggc gggttttcac cgacctccag
    15541 agccttctcc gccgcgaacg gcattcccag atccgcgtcg ctgtcgcgtc caagcttggg
    15601 tactggtggc ggcgggatct caacctgact ccggcagaaa aggaaattgc ccgacagacg
    15661 tgggaacagt acgtgcggca gatgacgacc gcccatgcgg acgatccgat tgctcgcaat
    15721 acgctgggaa tggtctacat gcgtcgggga gagtttgaca aagcgattcg ggagttccag
    15781 atttcgttga gaatcgaacc gggcagttcc ggtgtcgaaa ggaatctggc tgacgtgtac
    15841 acggcaatga atcggtattc ggaggcgttg ccacacaacc agcgtgcagt tgatttggaa
    15901 ccggacaacc ctcacctgta cgacgcgctt tcatttacgt atctgggtct ggggaggatc
    15961 gctgacgcgg aacgcgaagc tcagcgggct ctccggctgg atccccgttt tgcgccgggg
    16021 tatgttcaga tggcgcggat acgccacgct cagggcaaca tcgccgaagc gcatcggctg
    16081 cttcaaaaag cagttgctct ggacgcgcgc aatgttgagg cccaatatct gctggccaag
    16141 gcgagtcagg agttgcgatt gcgggaccaa gccgttcagg ccttcgtgaa cgtgatttcc
    16201 ctggatgagt tttcggagtt cgggtacgac gcaaagaacc agttggctac gatgcttccc
    16261 gttggggccc ccttgccgcc gccgacggca acgcccgccc ctgaacggcc ggcgcaggaa
    16321 gggccgatga ggccaatcga cccacatctg ctgtggccta ccgttgtgta caagagctac
    16381 gcaccgaaaa caaccccgga ggaggtcgct cgcatcccgg agccttcgag ttttgcggcg
    16441 tctctcgatt caggacgcgc atggatgcgg ctcgcggaaa acagccgcct ttccagtgcg
    16501 acgaaaaaag catacataga tcgttcggtc gcaacgctct cacgggctca ccggatggcg
    16561 ggatcggatc gttcaaagga cgtcgaggtt cgaaaagctc tgggatacgc tctttcggtt
    16621 caggcggaac acgtgcctcc ggcaaacgca cccgcgttac tcgcccgggc gagaagcgtt
    16681 ctggatggtg tcgacacaac ggcgctcggc ggggacgccg ctgcgaaggc cagcctcgga
    16741 agatcctatt accgtcttgc cagagcgtat aacgaaacgc gggactatcg aaacgctatc
    16801 tgggcgtttg agcgcgcggc agtgctcgta ccgaactcat tcactgaagc aatttcgcat
    16861 ttcttcgtag gcacctcgta cgaccgtctc ggccaacggc aggaagccat cgcggcgtac
    16921 cagcgatctg cctccaatcc gcataactcg ggacgtggcc tgcggtgttc tctccatggt
    16981 atcaagtacc cgttccggta tttccgctga aatcctgatc caccaccggc aaaaaggaac
    17041 gagcggcctt ttcgggccgc tcgttctcct cagaggaaac acctgcttat cgtgttccgg
    17101 caacccgcct cactgcctca gtgacctgtc ggacattcgg agaaacggga atcccactga
    17161 gtatgaaacg aaccgtgtta tcaggcgcga ccaccatgac caccactctg gagcgatcat
    17221 tccctctcca ggcagtcgac acagagccgt cccagt
//
LOCUS       DAOSOO010000048        17025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3391_length_17025_cov_4.773129, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000048 DAOSOO010000000
VERSION     DAOSOO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_17025_cov_4.773129"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLT86_05245"
     CDS             complement(<1..823)
                     /locus_tag="PLT86_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOT36049.1"
                     /translation="MDRICVKPELFVWARERAGLDRYALAASFPKLPKWESGELQPTL
                     RQLEAFASAVHVPFGYLFLPDPPDEPLPVADFRSIAGRETARASPNLLDTLYLCQQRQ
                     EWYRDYARLAGLPALSFVGSAGIEDNPVDVAEEMRSTLAISVEERAEIPTWTAALREL
                     VANAEDAGVLVMSSSIVGNNNRRKLQVEEFRGFALADEYAPLVFLNAADSKAALMFTL
                     AHELAHIWLGESGVSDVTAGQVPRQRTERWCNEVAAELLVPMEELRTQFRGGEPLQ"
     gene            complement(929..1894)
                     /locus_tag="PLT86_05250"
     CDS             complement(929..1894)
                     /locus_tag="PLT86_05250"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOT36050.1"
                     /translation="MTAVWCVRANAGEYAGHFLRGSYVAIGWREVAHELSTVRTREEL
                     YSIMRAAYPDVSSPIVIGNYVGQVARFILEMKAGDYVITPAADTEELHYGRLAPDPSY
                     FFATGDDGCPYRHRRRVEWASGTLKRSGLSVPLQNTIRSSLTVFAVSQREEFLAAIGS
                     PTQQTATAYDPYRAVLERVLQLDDKEFEILVGHLLTALGFDGSEVTGKTGDGGVDATG
                     ELNVANLAKIKIFVQAKRYKLGSKVSASVVKQLRASIPSNGQGAFITTADFQTSAADV
                     ALQTGFPRIGLVNGRQLVDLLVEHWNDIPADFRDQLGLKPGLVRV"
     gene            complement(1894..2400)
                     /locus_tag="PLT86_05255"
     CDS             complement(1894..2400)
                     /locus_tag="PLT86_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36051.1"
                     /translation="MARKKSPGSRSIESYDHADKTRTNKTRGISIRAFLVSSHGGFDP
                     VCAEAERWDLRLPPRVSGHFEALKNQGFASLGTHSAASGLNHNPACMNNQVVPLAART
                     ASFETMPASLSCGANYVETESRTLAALRDTLLPKLISGQIRLPAAFCECFAQAGVKGT
                     GRSVGEVV"
     gene            complement(2569..2650)
                     /locus_tag="PLT86_05260"
     tRNA            complement(2569..2650)
                     /locus_tag="PLT86_05260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Leu,seq:taa)
     gene            complement(2667..3719)
                     /locus_tag="PLT86_05265"
     CDS             complement(2667..3719)
                     /locus_tag="PLT86_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT36052.1"
                     /translation="MTEPTAPRVFITGGAGFLGINLIRYLLPKGFGIISYDKADFTYP
                     ERDQVTVVTGDIRDFDTLKAAMADSSIVVHCAAALPLYSEKDILSTDIDGTTNVLEAA
                     HQNGIQRVVHVSSTAVYGIPDHHPLREDDPLEGVGPYGIAKVEAEKVCLEYRKKGMCI
                     PIIRPKSFVGPERLGVFALLYEWAKDGKNFPMIGNGRNRYQLLDVEDLCEAIYLCMTG
                     DPAKVNDVFNIGAREFTTMREDYQAVLDRAGFGKRIIGFPAAPVIWTLRFLEMLHLSP
                     LYKWVYETAATDSFVSIEKAERVLGFSPRFSNKQALVRNFEWYLEHLSEFENVTGISH
                     RAPWKQGILKLFKVFF"
     gene            3922..4716
                     /locus_tag="PLT86_05270"
     CDS             3922..4716
                     /locus_tag="PLT86_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT36053.1"
                     /translation="MAHPPVLERCCYSDVQSLEYEVAVLPIGATEPHGKHLPYGQDFL
                     SVKAIAEEACRRANDGGARTIALPTIPYGVDTNQLGFPFAMSLYQKTLNVIVDDLIES
                     LVCHGVPKLVILNGHGGNEFKSYLREQCRNPRMFVCSINWWTVAEDAYKDIFTHRDDH
                     SGEMETSVALHLMPELVHLERAGDGSTRETDFEGMNSGWVQIARPWHLFTKDSASGDP
                     HLATAEKGVRYAEICISRIAGFLSQLSATPRDDRFPYSRAFATEGR"
     gene            complement(4724..5347)
                     /locus_tag="PLT86_05275"
     CDS             complement(4724..5347)
                     /locus_tag="PLT86_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT36054.1"
                     /translation="MVFGRLSPRCFSCVFGVALLLLPGLAGAVSYVGLGGGMSPHINT
                     TVVSNYSTGLDTVKFRGGYGFEAQFGWEAAPWIDIEGALRYHLSGKADTLRADREIVS
                     GLDIIGAEGGVRLHPRRGWSNSGPYLRGGIGSFSTTLSLDKGAASQNGEPALGYYAGI
                     GYVHEISGSWGVDLRATYIRFNAFDMNADGRKFRAGFLAVTASLVVF"
     gene            5488..7329
                     /locus_tag="PLT86_05280"
     CDS             5488..7329
                     /locus_tag="PLT86_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOT36055.1"
                     /translation="MFRKLLCVACAVAVTGCGNARRTTVVGAASALSYTLSAAKPEGG
                     FGPVFQPYRGVHWTFFGLAILRTAGAPIPNRVETASFLREQPAGPQLSEPDFQRVLAL
                     HMLGFVSDDSTISGLKATQGLDGGWNGSIGATYRAIELLTTLGAKPVFPEKAAQFFVD
                     RLSQDGFFREIPRPGTRSDSLRIASLRNDSTSESSALLWTEHIWNTYCAVQTLTKLGY
                     EVPWRPRVRAWLQSCQNQDGGFSFLPASQVTQSSDVWYTWLAIDALRALGAVPADSLG
                     AVAFINACQNADGGFGDRPGRQSRLESTYYAIAALNSLCGGVEAGLKEKVRVQPPLIR
                     RDGSRDYTANYLYVPSVDRVPLRGFRSLQGRGLPLLAMGDDIVSTDSVRGERAAGATG
                     SVQVSGLVETFPRSVIVRLPGNVWAGCRVSFRIGAAQKSVLMGLLRDSESEWAVGTAP
                     SGIPWGVFLERYVTPFVTRGALPVLQIEPGDQAGIYALTEAVGTGLSSDVAVAAALDD
                     GTDLARTCPRIERLNGLVPFTVALGGVSREENARTLWLGNKPNSEGFLEALKNGRAVC
                     AVRTGDSPTGVVFYGDPGAIAFAAAHPQAWAWWDMPAHLRTTHSSVR"
     gene            7377..8360
                     /locus_tag="PLT86_05285"
     CDS             7377..8360
                     /locus_tag="PLT86_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36056.1"
                     /translation="MKKVRIGFIGCGGVSNGHAYRIAGMPDAQIVALCDTNEATLKTF
                     KERHPELAKTPTFGDWRKMLEAVKLDAIEIHTPHTLHFEQIMAGLDHGLHVLTEKPMV
                     CKTEHARAVVKKAKETGLKVQVSYQRHFDRGFRFAKELIAEGKLGKIQFVAAQQSQEW
                     KRATAGSWRQIPELSGGGQLNDSGSHLVDIVLWVTGLVPEKVTAVIDNCGTPVDINSA
                     VSVQFKGGAQGTFSIVGEGLRWWEDVTIWGEKGIIAFRNGFPTMYVSEDGEVHLPQRM
                     PGGTDPDRNFVDAILGKAELESPAECGLRVIQLTEAAWESAALGKAVKVKR"
     gene            complement(8416..9828)
                     /locus_tag="PLT86_05290"
     CDS             complement(8416..9828)
                     /locus_tag="PLT86_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOT36057.1"
                     /translation="MRTETDALGKLTLDANTRYGIHTARAVASFGPADHPVPRGLVHA
                     YADVKRACARVNHRLGYLTDEQATALEAACSEMAAGLLDEWIVVDAFQGGAGTSTNMN
                     VNEVLANTALLTTGRECGDYGWLHPIDHVNLHQSTNDTYPTALRVAVIRQLRTLSDTL
                     VQLVESFQQAEKRFADVAKVGRTEMQDAVLITLGREMGAYAEAFSRDRWRVFKCEERI
                     RVSNLGGTAVGTGITAPRQFIFQATEELRTITGLPIARAENLVEATQNQDAWVEVAGI
                     LKALASNLIKIGNDLRLLSSGPHAGLREIHLPPMQEGSSLMPGKVNPVIPEMAVQTGM
                     ATFGFEHMISLAVSAGNLELNAFMPLIAYAMLTSLDLLERAVDRLARLCVHGIEADRE
                     QCANYVITSHAVATALVPVLGHDATARLVAEAERSGRSLRDVAVSSRALTAEEWEDLT
                     SVERVNALGSPVVRKRREEN"
     gene            9963..11030
                     /locus_tag="PLT86_05295"
     CDS             9963..11030
                     /locus_tag="PLT86_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36058.1"
                     /translation="MPKLPITRRPVKGALFGFGSIGPVHLYSVRGTPDGSIHPVPDVE
                     VVGVAEPVPERASTIPSGVEVFPDYQTLLRQADVDVVHICLPHHLHAPATIAAAEHGK
                     SVICEKPMALNASEARMMSDAIASNGVGFSLISQNRLNGEKAWMKEKVGAGDLGTLTQ
                     IDWTVDWFRSAEYYDAGSGWRGRDSEARGGVLSNQAYHTLDLVMWLADSPVTEVVAEC
                     STDKVLHPGIDVPDRVEGELHFENGILSRFMCTVCGDKRDVIRVELRGSVDGKDAFIV
                     ADGNSIVEQNLVPESPRFAVEGAVLGKACYGSSHQTNIALSYEAFMGEGTVPVTPEAG
                     ISVLRVIDAILESNGKPVPVL"
     gene            complement(11073..12134)
                     /locus_tag="PLT86_05300"
     CDS             complement(11073..12134)
                     /locus_tag="PLT86_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36059.1"
                     /translation="MAKGQTYNYERIPRVDPITGTRYMQLTSFPTINMHLSYCYPNLT
                     NGFTADSRRVVFLSRRAAHRTAPWDVFRVGVDGADFVQLTEREGVRGAVVSSQKPLVY
                     FHSNGTLYSVDMDTFEEEEISHFETDKSQHGGRFSGWLSADERFCFVSAFDKSGYELI
                     VRHATDGSGTAAIASAAEMRIHSIDPAGRGLFVSLNENGRWIIWLVDFDGARVVRYGE
                     NVCSHLSPLGNTGALQGCAVWPEHSLLLLTPGQERENRLLSGPDFWHSSASPDGRWIV
                     ADTNRPNEGIFLVNVATRRSAVLLHPHNVGGDAPWSHAHPFFSPDMRYVAFTTDATGS
                     AQVCLVEIPASLTEQLARP"
     gene            complement(12251..13462)
                     /locus_tag="PLT86_05305"
     CDS             complement(12251..13462)
                     /locus_tag="PLT86_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT36060.1"
                     /translation="MVASAGDSLVRQFEFATASRIVFGRGRANEIGSLASDLAGMTGS
                     EKRAFLIIGMTSADGNSGIAAIPEILRSQGFRVAEHRCSGEPTVSAVRLATAAGREHA
                     PHVVVAVGGGSVLDTGKAVAALLSNAGDPEDYLEGVGTGRTFERPAVPLIAVPTTAGT
                     GSEVTRNAVVKADDGSFKKSMRSAFLLPKCALVDSNLTHSLPERSTTYTGMDALTQLI
                     EAFTSNAANPLTDCLAIRGIQLVATSLERVVADGSCADARDDMSLAALLSGICLANAG
                     LGAVHGLVAPLGARFSVPHGAGCAALLAPVIEANSRALRRQGASHPCLEKYRTVEEAF
                     ALRSRAANKGLAQIIRALVTDLRVPGLRYWGVSLEAIPDLVKKSRGGSMRYNPVELGD
                     EELATIIEAAL"
     gene            complement(13456..13764)
                     /locus_tag="PLT86_05310"
     CDS             complement(13456..13764)
                     /locus_tag="PLT86_05310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOT36061.1"
                     /translation="MYVVCVTVHVKPEHVAEFAAATKTNARSTREEKGNFRFDILQSL
                     ADPAHFLLYEVYESEAAFQEHQKTAHYLSWRDVVADWMAEPRSSVKCAAVFPTEPQEW
                     "
     gene            complement(13776..15503)
                     /locus_tag="PLT86_05315"
     CDS             complement(13776..15503)
                     /locus_tag="PLT86_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36062.1"
                     /translation="MRRPEIQLALDPAPERVKVLLFATPPIGYVAETAPEWAEKGFSG
                     FIRPDIMKGWNADVWTLPEGRRIPGNDNPFLQQCRSMNQALAGAGLRDNFMCVPFTVE
                     LPDWFDDERWAAVCENFRQGARFARLAGFRGMALDDEYIEDSLGLNWARYQKPGYPVD
                     KLLPQARFRGQQIQNAMLSEFPDMVTLHLPESYSIMGDLAKALFLGYLDALCAADAPG
                     GLDILPECTYFQTDPWWIALYGAGLDQILREDLQTLDSSGRLSDYWDRRCGIALGQAP
                     LGYLRFVRDADGKRIGYGGRNEVFGDRIIEGGEDKSGNYPASVFRETHAAVRTVSRRY
                     AWVFCPGPVWWQMSEDQWRRHGGPHVAALPLVPEFADYTLPLRQPEIAVGAAYDTLRN
                     AVKTRTSANLLSGLGMPPSWWVNGPYDNSGGKGYDCVLAPEQGIDLDMASLGVWGEVR
                     WKQVRTPPTGYVDLSRLIAGGSEIQGYAATWCETETEISVVVHFGCDDTGKIWCNGNL
                     VHESKTERIAVPDEDTFPLVVPPGRSVFLLKIGNYRGGWGFYFRITDCKGDEIPSLRW
                     DLPLPLNAT"
     gene            15635..16963
                     /locus_tag="PLT86_05320"
     CDS             15635..16963
                     /locus_tag="PLT86_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36063.1"
                     /translation="MDTVRIEDDIPVVSRPDVLVAGGGFAGICAAVAAAEQGADVLLV
                     EGFAELGGAMAVSGVSGFCGHTRGVGRVFDEIIAVLEEARAIAPYHPEQDARAVETVY
                     VGFLMQEYALRKGVTLLLHSRVAATRRDGNIVDTVFLHTAGGLQAYCPKLIVDATGDA
                     DVAFAAGYPTVSGSESGGAPLPMSLYFSMWDTHCEQTPWLPPGCPRYTAETIPMTSIH
                     RHENGRIDVKMKVIGHSAVNARELSDAEIEARRQMMGLVYFLQTEGYSGRRYSTYCLS
                     SISPLIGIREGRRIIGDVVLTEQDVRAGREWEDAVVVGTYHVDYHWPTVLQRAGTGIT
                     DAVPPYHIPLRALIPRGSENLLVAGRCASGDQMAMSSFRVMATAAGMGIAAGTCGCLA
                     LREGCAPRDVDVGELQDLLRRNGVVLDCSWHSIYQQERRRRAMEATAETP"
BASE COUNT         3376 a         4876 c         5247 g         3526 t
ORIGIN      
        1 cttgcagagg ttccccaccg cgaaactggg tgcgcagctc ctccattggc accagcagtt
       61 ccgcagcgac ctcgttgcac caacgttctg tacgctggcg tggcacttgc cctgctgtga
      121 catcggacac cccgctctca cctagccaga tgtgcgcgag ttcgtgcgcc aatgtgaaca
      181 tcagggctgc cttgctgtcc gcagcattga gaaacaccaa tggagcgtac tcatccgcca
      241 gagcgaaacc acgaaactcc tcgacttgga gctttcgtcg gttgttgttg cccacgatgg
      301 agctcgacat caccagcaca ccagcatctt cggcgttggc gactaactcc cgcagggcgg
      361 cggtccaagt ggggatctcc gcccgttcct caacagagat tgcgagggta ctccgcattt
      421 cttcggctac gtcgactgga ttgtcttcga tacctgcgct acccacgaaa ctcaacgcgg
      481 gcaagcctgc taagcgcgcg taatccctgt accattcctg gcgctgctga caaaggtaga
      541 gtgtatccaa gagattcggg ctggcgcgcg cggtctcccg gcccgcaatc gaccggaaat
      601 ctgcaaccgg caagggttca tcaggtggat caggcagaaa caggtacccg aacggaacgt
      661 gaactgcgct ggcgaaggct tccagttgcc tcaacgtggg ctggagttcg ccgctttccc
      721 atttgggcag tttcgggaaa cttgcagcca acgcgtacct gtcgaggccc gcgcgttcac
      781 gcgcccagac gaacagttca ggtttgacac agatgcggtc catacaaatc aatctaacaa
      841 tttctccccg tccttcaccc ctatctccgg cggaccggct tcggcggcgg tgcctcgtct
      901 tccacgacag actcaacgaa actgccgctc ataccctcac cagccccggt ttgagtccga
      961 gctgatcccg aaaatctgcc gggatatcgt tccagtgctc aaccagtaag tcaacgagct
     1021 ggcgaccatt gacgaggccg atgcgaggga acccggtttg tagcgccacg tcggcggcag
     1081 atgtctggaa gtccgcagta gtgataaacg cgccctggcc gtttgacgga atcgaagcgc
     1141 gcaactgctt gacgacactg gcggaaacct tcgagccgag tttgtaccgc tttgcctgca
     1201 cgaatatctt gattttcgcc agattggcga cattgagctc ccccgtggcg tccaccccgc
     1261 cgtcgccggt ttttccagtg acttcggagc cgtcgaagcc cagcgcggta agcaggtgtc
     1321 ccaccagtat ttcgaattcc ttgtcgtcga gctggagtac tcgctcgagt acggcgcgat
     1381 aagggtcata tgcggttgcg gtctgttggg tggggctgcc gatggctgcc agaaactcct
     1441 cgcgctgcga caccgcgaac accgtgaggg aggagcggat cgtgttctgc aacggcaccg
     1501 aaaggccact gcgcttcagc gttcccgacg cccactcgac gcgccggcgg tgcctgtaag
     1561 ggcagccatc gtcaccggtc gcaaagaagt aggagggatc gggtgcgagc cgaccgtagt
     1621 gcagctcttc ggtgtccgcg gccggcgtga tcacgtaatc tccggccttc atttcgagga
     1681 tgaagcgcgc tacctggccc acgtagttgc cgatgacgat ggggctgctc acgtccgggt
     1741 acgcggcccg catgatcgag tagagttctt cgcgtgttcg caccgtggac agttcgtgcg
     1801 cgacctcacg ccagccgata gcgacgtagg aaccgcgcag aaagtgcccg gcgtattcgc
     1861 cagcattcgc gcgcacacac catacggcag tcatcacacc acctccccca cagaccgccc
     1921 cgtacctttc acccctgcct gagcgaagca ttcgcagaac gcggcaggca gccttatctg
     1981 ccccgaaatc aacttcggca gcaacgtgtc gcgcagcgcg gcgagggtgc gggattctgt
     2041 ttccacatag tttgcgccgc atgagagact cgcaggcatg gtctcgaagg aggcagtccg
     2101 cgcggccaga ggcaccactt ggttgttcat gcatgctggg ttgtggttca ggccggacgc
     2161 ggcggaatgc gtaccgaggg aggcgaaacc ctggtttttg agggcctcaa aatgaccgga
     2221 gacgcgaggc ggcagccgaa ggtcccacct ttccgcctcg gcgcaaaccg ggtcaaaacc
     2281 cccgtgcgac gacaccaaga acgcccgaat cgaaatcccc ctcgttttgt tcgtgcgcgt
     2341 cttgtcggcg tggtcgtagg attcaatcga gcgggaaccg ggggacttct tccgtgccat
     2401 caggggtctc ctcgctggtg cgttttccat acggttagat agtactcaat cccgctttca
     2461 aatcaaccca atgggcggtg cactccgaga agcaagggcg cggttgaagg gacaagcggg
     2521 gtgttgtgct ggacgaaggg aattagtgcg cgggagtgta gtggtgagtg ccccggtcgg
     2581 gactcgaacc cgaacggctt tgggccagcg gattttaagt ccgcagcgtc taccagtttc
     2641 gccaccgggc gattgtgagc gagagctcag aaaaaaacct tgaagagctt gagaatcccc
     2701 tgtttccacg gggcgcggtg actgattccg gtcacgtttt cgaattcgct caggtgctcg
     2761 aggtaccatt cgaaatttcg cacaagcgcc tgcttgtttg aaaaccgtgg tgaaaaaccg
     2821 agaacgcgtt ctgctttctc gatggagacg aacgagtcgg ttgccgcggt ttcgtacacc
     2881 cacttgtaca ggggagacag gtggagcatc tccagaaacc ggagggtcca tatcaccggt
     2941 gcggccggga atccgatgat tcgcttcccg aaacccgccc gatcgagcac ggcctggtaa
     3001 tcctcccgca tggtggtgaa ctccctggcg ccgatgttga acacgtcgtt cacctttgcc
     3061 gggtcgccgg tcatacacag gtagatcgcc tcgcacaggt cttccacgtc gaggagctgg
     3121 tacctgttcc tgccgtttcc gatcatcggg aaattcttgc cgtcttttgc ccattcgtag
     3181 aggagcgcga acacgccgag gcgttccggg ccgacgaatg atttggggcg gatgatcggt
     3241 atgcacatgc ccttcttgcg gtactccaga cacacctttt ccgcttcgac ttttgctatg
     3301 ccgtaggggc cgacgccttc caggggatca tcttcgcgga gcgggtgatg gtcaggtatg
     3361 ccgtaaaccg cggtggaaga gacgtggaca acccgttgga tgccgttctg atgggcggct
     3421 tccagaacgt ttgtggttcc gtcaatatcg gtcgagagga tgtctttttc tgagtacagg
     3481 gggagagcgg cggcgcagtg gacgacgatg gaagagtcgg ccatcgcggc tttcaacgtg
     3541 tcgaagtcgc gaatgtcgcc cgtaacgaca gtgacctggt cgcgttcggg gtacgtgaaa
     3601 tccgctttgt catacgaaat gatcccgaaa cccttcggga gcaagtagcg gatcaggttg
     3661 atcccgagaa aacctgcgcc tccggtgatg aacacgcgtg gggctgtggg ttccgtcatg
     3721 cttcgttccg tctggcgggt gtgatgtgcg gtgcgtggat atccggacag ttgttcgtgc
     3781 tcagaatcga attcagaact gaaatatcgg cccgcgggct ttcgtttggc aacggaatgg
     3841 caccggtgga acgcaccggg tgaggccggt atctttggaa ggaaataccc atgcgtgctt
     3901 ttgagacaca gggaggaaga aatggcccat ccaccagtcc ttgaacgatg ctgttattcg
     3961 gatgtacagt cactcgaata cgaagttgcg gttctaccga ttggcgcgac cgagccccac
     4021 ggcaaacacc tcccctacgg ccaggatttc ctgtctgtga aggcgattgc ggaggaggcg
     4081 tgtcgaaggg ccaacgatgg cggggcgcga actattgcgt tgccgacaat cccgtacggt
     4141 gtggatacga accagctcgg tttcccgttc gcgatgagtc tttatcagaa aacgttgaat
     4201 gtcatcgtgg acgatctcat cgagtccctc gtctgccatg gtgttccaaa gctcgtgatt
     4261 cttaacgggc acggcgggaa cgagttcaaa agctatctgc gcgagcaatg ccgcaatccc
     4321 cggatgttcg tgtgcagcat caactggtgg acggtggctg aggacgcgta caaggatatc
     4381 ttcacgcacc gtgatgacca ttcaggagag atggaaacga gcgtagccct gcaccttatg
     4441 cctgagcttg tccatctgga gcgcgcggga gatggatcca cgcgagagac ggacttcgaa
     4501 gggatgaact ccggctgggt tcagatcgcc cgtccctggc acctgttcac gaaggattca
     4561 gcctcgggag accctcatct tgccacggca gaaaagggcg ttcggtacgc ggagatatgt
     4621 atctcgcgaa ttgcgggttt cctgtcgcag ctttcggcta ccccgcgtga cgaccggttt
     4681 ccttactcgc gggcattcgc gacggaggga aggtagagag gggtcagaat acgaccagcg
     4741 aagccgtgac ggcgagaaac ccggcgcgaa acttccgtcc gtcggcgttc atgtcaaacg
     4801 cgttgaaccg gatgtacgtt gctcgcagat caacgcccca ggagccgctg atttcgtgca
     4861 cgtagccgat accggcgtag tatccgagcg ccggctcgcc gttctgtgat gccgcaccct
     4921 tgtcgaggct cagcgttgtg gagaaagagc cgattccccc tcgcagatac ggtccgctgt
     4981 ttgaccagcc acggcggggg tggagcctga cacctccttc ggcgcctatg atgtcgaggc
     5041 cgctgacgat ttcgcgatcc gcacgcaggg tgtcggcttt tcccgagagg tgataccgca
     5101 gcgcgccctc gatatctatc cacggtgcgg cttcccagcc gaattgcgct tcaaaaccgt
     5161 accctccacg gaatttgacg gtatccaacc ctgtcgagta gttggaaacg acggtcgtat
     5221 tgatgtgcgg agacatacca ccgccgaggc cgacataaga gaccgcgccc gccagccccg
     5281 gaagaagcaa cagcgcgacg ccgaacacgc aggagaaaca gcgaggggat agtctgccga
     5341 aaaccacgct ccgatccttt ccaaggttgc cagcgcaagg gagacaataa caaaatgacg
     5401 cactgccgtg gcgcgtttac aacggcgcac ggttgcggtt ccccggtgaa gctcgtacgc
     5461 ttggtatgat ttctggagca cctccgcatg tttcgaaaac tgctttgcgt cgcctgcgca
     5521 gttgccgtca ccgggtgtgg aaacgcacgc cggaccacgg tcgtcggcgc agcatccgcg
     5581 ctctcttata cgctgtccgc ggcgaaaccg gaaggcggtt tcggtccggt ctttcagcct
     5641 taccgcgggg tgcactggac gttcttcgga ctggcgattc tccggaccgc aggcgctcca
     5701 attccgaatc gcgtcgaaac ggcatcgttt cttcgagaac aacccgccgg gccgcagctt
     5761 tcggaaccgg attttcagcg cgtgcttgcg ctccacatgc tgggattcgt ttccgatgac
     5821 agcaccatct ccggtttgaa agcaacgcag ggactcgacg gcgggtggaa cggcagcatc
     5881 ggcgcaacgt atcgcgcgat agaactcctg accaccctgg gcgcgaaacc tgtctttccc
     5941 gaaaaggcag cgcagttctt cgtcgacagg ctttctcagg acggcttttt ccgggaaatt
     6001 ccccggccgg gaacgagatc ggacagtttg cgaatcgctt cgcttcgaaa cgattcaacg
     6061 agcgaaagct cggccttatt gtggacggaa catatctgga atacctattg tgcggttcaa
     6121 acgctgacga agctcgggta cgaggtaccc tggcgcccgc gggtaagagc gtggctgcag
     6181 tcctgccaga accaggatgg gggattctcg tttcttcccg catcacaagt gacacagtcg
     6241 agtgatgttt ggtacacgtg gcttgcgatt gatgctctcc gcgcgctggg ggccgttccg
     6301 gctgacagcc ttggagcagt ggcattcatc aacgcatgcc agaacgcgga cggggggttc
     6361 ggggatcggc cgggaaggca atccaggctg gaatcgacgt actatgcgat tgcggcgctg
     6421 aattcacttt gtggaggggt tgaagccggc ctcaaggaga aggtgcgcgt tcagccgccg
     6481 ctgattcggc gtgatggaag ccgggattac acggccaatt atctgtacgt gccttccgtg
     6541 gatcgggtac cgttgcgggg gttcaggtct cttcaggggc gtggcttgcc tctgttggct
     6601 atgggcgatg acattgtatc gacggattcc gtgcgcgggg aaagagcggc tggcgccacc
     6661 ggaagcgtgc aggtgtcagg gctcgttgag acgtttccgc gatctgtcat cgttcgactt
     6721 ccggggaacg tgtgggccgg ttgccgtgtc tcgttccgga ttggagccgc gcagaaatcc
     6781 gttttgatgg gacttctgcg cgattctgag agtgagtggg ccgtgggaac ggctccatcg
     6841 ggaatcccct ggggagtgtt tctggagcgg tacgtgacgc cgttcgttac ccgaggcgca
     6901 ctgccggttc tgcagatcga accgggggat caggcgggga tctacgcgct cacggaggcc
     6961 gtagggacgg gattatccag tgatgttgca gtggcagcgg ctctagatga tgggaccgac
     7021 cttgcgcgga cttgtcccag aattgaacgg ttgaatggtc tcgtgccgtt cactgttgcg
     7081 ttaggcggcg tttctcggga agagaacgct cggaccctct ggctcggaaa caaacccaac
     7141 tcggaaggtt ttcttgaagc tctgaagaat ggccgcgccg tgtgcgcggt tcggacaggt
     7201 gattcaccca cgggtgtggt tttctacgga gatcccggcg caatcgcatt tgcggctgcg
     7261 catccgcagg catgggcctg gtgggatatg ccggcgcatc tgcgcacaac gcattccagc
     7321 gtccgttaat gtaggtggtt ggttgtcgta cgcgtgttac actctggagg aaacaaatga
     7381 aaaaggttcg aatcgggttc atcggttgtg gaggtgtgtc aaacgggcat gcctatcgca
     7441 tagcgggaat gccggacgct cagattgttg cgctgtgcga caccaacgaa gccacgctga
     7501 aaacgttcaa agaacgccac cctgaactgg cgaaaacccc gacgtttggc gactggcgca
     7561 agatgctgga agcagtgaag ttggacgcta tcgagattca cacacctcac acgctgcatt
     7621 ttgaacagat catggcgggt ctcgaccacg ggcttcacgt tttgacggaa aagcccatgg
     7681 tctgcaaaac ggagcatgct cgagcggttg tgaaaaaggc caaggaaacg ggtctgaaag
     7741 ttcaggtcag ttatcagcgg catttcgatc gaggttttcg attcgccaaa gagctgattg
     7801 cagagggcaa gctgggaaag atccagtttg ttgcggccca gcaatcccag gaatggaaga
     7861 gggcaacggc cggcagttgg agacagattc ccgagctttc gggtggcggc caattgaacg
     7921 attctggaag ccatctggta gacattgtgc tgtgggttac cggtctggtt cctgagaagg
     7981 tcacggcggt gatcgacaac tgcggcaccc cggtggacat caactcggcg gtgagcgtgc
     8041 agttcaaggg cggtgcccag ggcaccttca gcattgtggg agaagggctg cgctggtggg
     8101 aagacgtgac gatctggggc gaaaaaggca tcatcgcatt ccgcaacggt ttcccgacga
     8161 tgtacgtgag tgaagacggg gaggtccatc tcccgcagag aatgccgggc ggaacggatc
     8221 cggaccgcaa tttcgtggat gcgattctgg ggaaggcgga actggaatcc ccggctgagt
     8281 gcgggctgcg ggtgattcag ttgacggaag cagcgtggga atcggcggct ctcggcaagg
     8341 ccgtcaaagt gaagcgttga ttgaccttcg gtgcgttccg ggcccgcgcg atggcgtacc
     8401 gctgccgcgc gggcttcaat tttcctccct ccttttgcgc accaccggcg aacccagggc
     8461 attgacacgt tccacactgg taaggtcttc ccattcctcg gccgtaagcg cgcgcgaaga
     8521 aacggcgaca tctctcaggg atcttccgga acgttccgct tccgccacga gccgtgccgt
     8581 ggcgtcgtgc ccgagaacag gaacgagagc agttgcaacg gcatggcttg tgattacgta
     8641 gttcgcacat tgctcgcggt ctgcttcgat cccgtgcacg cagagacgtg ccaggcggtc
     8701 gaccgcacgt tccagcagat cgagactcgt aagcatggcg tacgcgatga gcggcataaa
     8761 tgcattcagt tccagatttc ccgcggatac cgccaagctt atcatgtgct cgaacccgaa
     8821 cgtcgccata ccggtctgga cggccatctc cggaatgacg gggtttacct ttccgggcat
     8881 gagggatgac ccctcctgca tgggcggaag atggatttcc cgcagacctg cgtgcggccc
     8941 gctggagagc aggcgaaggt catttccaat cttgatcagg ttcgatgcga gtgccttgag
     9001 gattccggct acctcaaccc aggcgtcctg attctgtgtc gcttcgacaa ggttctccgc
     9061 tctggcgatt ggtagccccg tgattgttcg cagctcctca gtggcctgaa agatgaactg
     9121 ccgcggggca gtgatgccgg ttccaacggc agttcctccg aggtttgata cgcggatccg
     9181 ctcctcgcat ttgaacacgc gccagcggtc gcgcgaaaag gcctccgcat atgcgcccat
     9241 ttcccgtccg agcgtaatca agactgcatc ctgcatttcg gttctgccca ctttggcgac
     9301 gtcggcgaac cgtttttccg cctgctggaa cgattccacc agctgcacga gggtatcact
     9361 gagggtgcgg agttgccgga tgacagcgac tcgcagcgcc gttggatacg tgtcgttcgt
     9421 cgattggtgc aggttgacgt ggtcgatggg atgaagccag ccgtaatcgc cgcactccct
     9481 gcccgtggtg agcagcgcgg tgttggcgag aacctcattc acgttcatgt tggtgctggt
     9541 gccggcaccg ccctgaaagg cgtcaacaac gatccactcg tcgagtagtc cggcggccat
     9601 ctcgctgcac gctgcctcca gagcggtagc ttgttcgtcg gtgaggtaac cgaggcgatg
     9661 attgacacga gcgcatgcgc gcttcacgtc ggcgtacgca tggacaaggc cccggggaac
     9721 ggggtggtcc gcggggccaa aacttgcgac tgcgcgggca gtatggatgc cgtaacgcgt
     9781 gttcgcgtcc agtgtcagct tcccaagagc gtccgtttca gttctcatct gtatcgttct
     9841 cactttcctc caggggtgcg ctgccgccgg cccgcgttga ctcgtacctg cgcgttgtcg
     9901 tttttcaaac aggagagcct cgtttcagtt cgaatgtcat tccgcagttt gaaaggattg
     9961 tcatgcccaa actcccaata actcgccgtc ccgtcaaggg tgccctgttc ggttttggtt
    10021 ccatcgggcc cgttcatctc tactcagttc gggggacgcc ggacggcagc attcaccccg
    10081 ttcccgacgt ggaggtcgtt ggggttgcgg aaccggttcc cgagcgtgcg tctaccatac
    10141 catcgggagt tgaggtgttt ccggattatc agacactcct ccggcaggcg gatgtcgatg
    10201 tcgtccacat ctgtcttccg caccacctcc acgcgcccgc gacgattgcg gccgcagaac
    10261 atggaaaaag cgtgatatgc gaaaaaccga tggcactgaa tgcttcagaa gcgcgtatga
    10321 tgagcgatgc cattgcatcc aacggcgttg gcttttcatt gatcagccag aaccggttga
    10381 acggcgaaaa ggcgtggatg aaagagaaag ttggtgcggg cgatctcggg acgctcacgc
    10441 agattgattg gacggtggac tggttccggt ctgcagaata ttacgacgcg ggatcggggt
    10501 ggcgtggccg ggattcagaa gcacggggag gagtcctgtc gaatcaggcc taccacacat
    10561 tggatctcgt gatgtggctt gcagattcgc ctgtcaccga agttgtggct gagtgttcca
    10621 ctgacaaggt tcttcacccc gggatcgacg ttccggaccg ggttgaagga gaactccact
    10681 tcgagaacgg catcctgtcc aggttcatgt gcaccgtctg cggggacaag agggatgtta
    10741 tccgcgtcga attgcgtgga tccgttgatg gcaaggatgc gttcatcgtc gccgacggca
    10801 acagcatcgt tgaacagaat cttgtgccag aatcgcctcg gttcgcggtg gagggtgcgg
    10861 tcctgggaaa agcgtgttac ggatcaagtc atcagacgaa catcgcgctg tcgtacgagg
    10921 cgttcatggg agagggaacg gtgcctgtga ccccggaggc cggcatcagc gttcttcgcg
    10981 ttatagacgc gatccttgag agcaacggca aaccggttcc cgtgctctga cgcgcggaga
    11041 cgtcatttgg agggataccg cccccaatat ttctacggcc gggcaagttg ctccgtcagc
    11101 gatgccggaa tctccacaag gcacacctgg gcgcttcccg tggcatcggt agtgaacgcg
    11161 acgtaccgca tgtccgggct gaaaaacgga tgggcgtgcg accacggtgc gtctccgccg
    11221 acgttgtgcg ggtgcaacag caccgcactt cgccgggtcg caacgttcac gaggaaaatc
    11281 ccttcgttcg gccgatttgt atctgctacg atccaacggc catccggtga tgccgaggaa
    11341 tgccagaaat cgggtccgct gagcaaccgg ttctcccgtt cctgtccggg ggtgagcaac
    11401 aggagcgaat gctcgggcca gacggcacaa ccctgcaggg ctccagtatt ccccagcgga
    11461 ctcaggtgcg agcatacgtt ctcgccgtac cgcacgacgc gcgcgccgtc gaaatccacc
    11521 agccagatga tccaccggcc gttttcgtta agggaaacga aaagcccgcg tcccgccgga
    11581 tcaatactgt ggatgcgcat ctcagcggca cttgctatcg ctgcggtccc cgaaccgtcc
    11641 gtcgcgtggc gaacgatgag ttcgtatccc gatttgtcga acgccgatac aaagcagaag
    11701 cgctcgtcgg ctgagagcca cccggagaat cggccgccgt gctggctttt atctgtttca
    11761 aagtgggata tctcttcctc ttcgaaggta tccatgtcta ccgaatacaa cgttccgttt
    11821 gaatggaagt agacaagggg cttctgcgac gagacgactg cgccccgcac cccttctcgc
    11881 tctgtgagtt ggacaaaatc agcaccatca acgccgactc ggaacacatc ccacggtgcg
    11941 gttctgtggg ctgctctcct gctgagaaac acgactctgc gggaatccgc cgtgaaaccg
    12001 ttcgtaaggt tcgggtaaca atacgacagg tgcatgttga tcgtcgggaa acttgtcaac
    12061 tgcatgtaac gggtacccgt gattggatcg acccgcggaa tgcgctcgta gttgtaggtc
    12121 tgccctttcg ccacagggat ctccttcggt tagcgatcga cggaacccgc gttgcccgct
    12181 cggaacggga ccatgccaca ttccgaaaaa cactcatgtt gttgtatgta cagcgatgcg
    12241 gccattctcg ttacagcgca gcttcgataa tcgttgccag ttcctcgtct ccgagttcca
    12301 cggggttgta ccgcatgctg ccgccgcggc tctttttcac gaggtcggga atggcctcca
    12361 gactaacgcc ccagtaccgc aggccgggaa cgcgcagatc ggtgacaagg gcgcgaatga
    12421 tctgcgcaag gcccttattc gcggctctgg aacgaagcgc gaacgcctcc tcgacggttc
    12481 tgtacttttc caggcacggg tggctcgcgc cttgccttcg cagcgcccga gagtttgcct
    12541 cgattacagg ggcaagcaat gccgcgcaac cggcaccgtg aggaacggag aaacgcgctc
    12601 cgagcggcgc aaccagcccg tgaacagcac cgagtcctgc gtttgcgagg cagatgccgc
    12661 tcaacaaagc cgcgagggac atgtcgtcgc gggcgtcggc gcaggagccg tccgccacga
    12721 cgcgttccag agaggttgcc acaagctgaa tgccgcggat ggcaagacaa tcagtgagag
    12781 ggtttgcggc atttgaggtg aaggcttcga tcagttgcgt cagcgcgtcc atccccgtgt
    12841 aggtggtaga gcgctctggg agcgaatgag tgagattcga gtccacgagc gcgcatttcg
    12901 gaagaaggaa ggcgctgcgc atgcttttct tgaaggaacc gtcgtccgcc ttgacgacgg
    12961 cgttcctggt cacctcagat ccggttcccg cggtggtcgg aacggcgatc agcggtactg
    13021 ctggacgttc gaacgtccgc cccgttccga ctccctcgag atagtcttcc gggtccccag
    13081 cgttggaaag caaagccgcg accgcctttc ccgtgtcgag aacgcttccg cccccgaccg
    13141 cgacgaccac gtgaggtgca tgttctctgc ccgccgcggt tgccagacga acagccgaaa
    13201 cggtaggctc tccgctgcac cgatgctctg caacgcggaa gccttggctt cggaggatct
    13261 cagggatcgc ggcaattccc gaattcccgt ctgcagaggt cattccgatg atcaggaagg
    13321 cccgtttctc gctgccagtc atccctgcca gatcagaggc aagagaaccg atctcgttgg
    13381 cgcgcccgcg accaaatacg atgcgcgagg cggtcgcaaa ttcgaattgt cgtacaaggg
    13441 aatctccggc tgacgctacc attcctgagg ctccgtgggg aacactgccg cgcacttgac
    13501 ggaggaacgc ggttcggcca tccaatcagc gacgacatcc cgccacgaca gataatgagc
    13561 agttttctgg tgttcctgaa atgcggcttc cgactcatag acttcgtaga gaaggaaatg
    13621 tgccggatcg gccagagact ggagaatgtc gaaccggaaa ttgccctttt cttctcgtgt
    13681 tgagcgcgcg ttcgttttcg ttgccgcggc gaattcggcg acgtgttcag gtttgacatg
    13741 taccgtcacg cacaccacgt acatctcagt gcctcctatg ttgcattcaa cgggagcggg
    13801 agatcccagc ggagtgaggg tatctcgtct cccttgcagt cggtgatccg gaaatagaat
    13861 ccccagcccc cgcggtaatt gccgatcttc aacaagaata cggatcgtcc gggcgggacc
    13921 acgagaggga aggtgtcctc gtcgggtacg gcaatgcgct cggttttcga ttcgtgcacg
    13981 agattcccgt tgcaccagat ttttccggtg tcgtcacacc cgaaatgtac caccacggag
    14041 atctccgttt ccgtctcgca ccacgtcgcc gcgtacccct gaatttcact gcctccggct
    14101 atgagccggc tgaggtccac gtacccggtc ggtggggtac gcacctgttt ccatcgcact
    14161 tcgccccaga cgccaaggga cgccatatcg agatcaatgc cctgttccgg agcgaggacg
    14221 caatcgtagc ctttgccccc ggaattgtcg taagggccat tgacccacca tgagggaggc
    14281 attcccaggc cggaaaggag gtttgcagaa gttcgggtct tcaccgcgtt ccgcagagtg
    14341 tcgtacgcgg cgccgacagc gatttccggt tgtctcaacg gcaacgtgta gtcggcgaat
    14401 tccggcacaa ggggcaatgc ggctacgtgt ggtccgccgt ggcgccgcca ctggtcctcg
    14461 gacatctgcc accagaccgg ccccgggcag aacacccacg cgtatcttcg agacacagtg
    14521 cgaacggcag catgtgtttc gcggaagact gaagccgggt aattaccact cttatcttcg
    14581 ccgccttcta tgattctgtc gccgaaaact tcgttcctcc cgccgtaccc gatccgtttc
    14641 ccgtcggcgt cccgaacgaa cctgaggtac ccgagagggg cctgtccaag cgcaattccg
    14701 cagcgccggt cccagtagtc cgaaaggcgc cccgaggaat cgagtgtctg gagatcttcc
    14761 ctcagaatct ggtcgagacc tgcgccgtag agcgcgatcc accagggatc ggtctgaaaa
    14821 tatgtgcact caggaagtat gtcgagtcct ccgggggcat ccgcagcgca gagagcgtca
    14881 aggtacccca gaaagagcgc tttcgcgagg tcacccataa tggagtagct ctccggaaga
    14941 tgcagggtga ccatatcggg aaactcggac agcatcgcgt tctggatctg ttgtccgcgg
    15001 aatctcgcct gtgggagcag cttatcgacg gggtacccag gcttctggta tctggcccag
    15061 ttcaacccga gtgagtcttc gatgtactca tcatccaacg ccatgccgcg gaacccggcg
    15121 agtcttgcga atcgagcccc ctgccggaag ttctcgcaca ctgccgccca cctttcgtcg
    15181 tcgaaccagt ccggaagttc gactgtgaac gggacgcaca tgaaattgtc acgcagcccg
    15241 gcacccgcaa gcgcctgatt catggaccgg cattgctgaa ggaacgggtt gtcgtttccc
    15301 ggtatccgcc ttccctcagg aagggtccac acatccgcgt tccagccttt cattatgtct
    15361 ggccgtatga aacctgaaaa gcccttttcg gcccattcag gtgccgtctc ggcgacataa
    15421 ccgatcgggg gcgtggcaaa cagaagaact ttgacccgtt caggggcagg gtcgagagca
    15481 agctggattt cgggccgtcg catggttcgt cctgtgcagt ggcgatgtca aatgagtacc
    15541 gtttcatagt atcgagtcag gtgtgccgcg gagcaacgtc acagcgctga attcccacgc
    15601 agcgaaagtt tctgcaggcg cggagggctt tgcgatggat acggtcagaa tcgaggacga
    15661 tattccagtt gtctctcgtc ccgatgtgtt ggttgccggg ggcggttttg cggggatctg
    15721 tgcggcagtg gcggcggcgg aacagggggc tgacgtgctt cttgtcgagg gcttcgccga
    15781 gttgggcgga gccatggcgg tttccggtgt gtctggtttc tgcgggcata cgcggggagt
    15841 cgggcgggta tttgacgaaa tcatcgcggt gctggaagaa gccagggcaa tagcgcctta
    15901 ccacccggaa caggacgcac gtgccgtcga aacggtgtac gtgggttttt tgatgcagga
    15961 gtatgcgctc aggaaagggg tcacgcttct gttgcacagt cgcgtggcgg ccacccgcag
    16021 ggacggcaac atcgtcgaca ccgtgtttct gcacactgcg ggcggccttc aggcgtactg
    16081 cccgaaattg atcgtggatg caacgggaga cgccgatgtg gcgtttgccg ccggatatcc
    16141 cacggtgtcg ggttctgagt cgggcggcgc tccgctcccg atgtcgctgt atttctccat
    16201 gtgggatacg cattgcgaac agacgccctg gttgccacca gggtgcccgc ggtacactgc
    16261 ggaaaccatc ccgatgacct ccattcacag gcacgaaaac gggcgaattg acgtcaaaat
    16321 gaaggtgata ggccacagcg cggtgaacgc gcgtgaactc tcagatgcgg agatagaggc
    16381 acggcgtcag atgatgggtc tggtttactt tctgcagaca gaaggctatt ccgggagacg
    16441 gtactccacg tattgtcttt cctccataag ccccctcatc gggattcgcg agggaaggcg
    16501 catcatcggg gatgttgttc tgacggagca ggacgtgcgg gcaggaaggg aatgggaaga
    16561 tgctgttgtc gtgggaacgt accacgtgga ctaccattgg cccaccgtgc ttcaacgtgc
    16621 gggtaccgga atcaccgacg ccgttccgcc gtaccacatc ccattgaggg ctctcatccc
    16681 gcgcggttcc gaaaatcttc ttgtcgccgg tcggtgtgcc tctggtgatc aaatggcgat
    16741 gtcgtctttc cgtgtgatgg cgaccgcggc cggcatggga atcgccgccg gaacgtgcgg
    16801 gtgcctggcg ctgcgggaag gatgcgcacc tcgggacgta gacgtgggcg aattgcagga
    16861 cctgctgcga cgaaacggag tcgttctcga ttgctcctgg cactcgatct atcagcagga
    16921 acgacgacgc cgggccatgg aggccaccgc cgaaacgcct tagacaggga tacagcatgg
    16981 aaaaccggga cgttaccgat agggaccgcg ctctcgctca gaagt
//
LOCUS       DAOSOO010000049        16969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3399_length_16969_cov_5.562788, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000049 DAOSOO010000000
VERSION     DAOSOO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3399_length_16969_cov_5.562788"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLT86_05325"
     CDS             complement(<1..691)
                     /locus_tag="PLT86_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36064.1"
                     /translation="MIVDAHAHPWWYGYDPDKLVANMDEHGIDVAWLLTWEVAEGMYD
                     HSYDRTWMAPYIGMPIEAVWDTVERYPKRFVAGYAPDPKSPMAIEKLKTAVKHGVRVS
                     GEHKFRTLFDDPDCIRFYRVCGELGLPVTLHIDVPCLPPDDPTKPVQWWYAGDISNLE
                     RALRQCPDTVFLGHAPGFWREVSGDADSDPNAYPKGPVKPGGRLRPLFDKYPNLYADL
                     SAGSALTAISRD"
     gene            1102..1935
                     /locus_tag="PLT86_05330"
     CDS             1102..1935
                     /locus_tag="PLT86_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT36065.1"
                     /translation="MIGQHVWLWLVPMGFVLGALGTLIGAGGGFVLVPVLLLMYPRNS
                     PETITSISLAVVFFNALSGSVAYGKMGRIDFRSGALFAAATIPGAILGAFTTDVIPRR
                     TFNGVFGVLMVAAAAYLILQRDKGPGHVGSGDAHKTVRRIVEKDGTVHTYSFSLRAGM
                     LWSTLVGYISSLLGIGGGIVHVPVLARLLNFPVHIATATSHFVLAIMALTGTVVHIAN
                     GSFAHGGFWRTLYLGIGVLLGAQLGASLSNRVKGVWIIRSLAIALGLVGVRILLMAFE
                     S"
     gene            complement(2000..2833)
                     /locus_tag="PLT86_05335"
     CDS             complement(2000..2833)
                     /locus_tag="PLT86_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT36066.1"
                     /translation="MTQEKPWVESDVWPSGRYLAAYLGIGVLVGLASALLGIGGGVLI
                     VPLLTGFLGVPIRRAVAISIVTVLLISFIGVARELQRGTTLPWLLVAVLAVGAQGGVW
                     LGGKVHDRISDRALRHVFMVVLLLTAAKMANLGGLGPARGIILLEDPVHWVAWVLVVG
                     ALAGLLSVLVGIGGGIVVVPGMALLIADIDFRVIQVVSLAMIIPTSLSGAYVHARRGN
                     VLWRSVVALSLPGCVGVLAGVALAHSMPSSWLKEIVFPVFLCLMVIRLGWMSRRQAER
                     G"
     gene            complement(2900..3511)
                     /locus_tag="PLT86_05340"
     CDS             complement(2900..3511)
                     /locus_tag="PLT86_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOT36067.1"
                     /translation="MANCGCDVEASNRGEHRTLVTVLAINALMFVLEFGFGVVAQSTG
                     LISDSLDMFADASVYAISLYAVGRSAALKARAAALSGFSQILLGAGASVEVARRFVFG
                     SDPVSSAMVAIGLVALVANVTCMMLIYRHRLGEVHMKASWIFSKNDVIANVGVIAAGI
                     LVGITGSRYPDLVIGLAIAVVVARGGVQILAEARAAGDGRTEC"
     assembly_gap    3768..3867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3893..>5965
                     /locus_tag="PLT86_05345"
     CDS             3893..>5965
                     /locus_tag="PLT86_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT36068.1"
                     /translation="MPQRFVRIAALVASLAAVLVPRAAEAATVSGYVRDKSSGESLPY
                     VQVFFEDLRTGAITNLAGYYAIPNVPPGNHLLRVAFLGYQDYTKEITVNGGVLVHNIE
                     LAEEAIEVAAVQVEAERVTAPSLTIAPSRTTLRMPDFRTAPALAEPDPIRVVQTLPGV
                     LSLSDFSVGLYVRGSTPDQNLLLLDGVSLYNVSHLFGLFSAFPADAVKSSELLKGGFP
                     AQYGGRLSSVLTINTDEGNKRTMVGKGGVSLISSRLTMEGPVAKGSWLVSGRRTYLEP
                     ILKLASRWNGTFGNLGYNFYDVQGKTHQVFSHEHQLSIAGYAGDDNLRFSDQTFDSQL
                     LWGNRSLSATWSYVPVDRLFFRTTGAIARYRSRLQLGIEDFGVVERNRLFDWTVKSDA
                     TVFLTEHNTVETGFEFICHTMNYTADFDKQTYNSFSIRTHHASAYAQTTLRPWAFLSV
                     QPGVRANYFDNGGYVDIDPRFSARYQIGENTYLKGAVGRYSQYLFRVAREFQGINFLS
                     DLWFTADSTAEPSHAWQYVAGLETKLTPDIDLTLEGYYKDFEMLAEFNSDAQTPMSAG
                     QMLRRGDGEAYGLEFGLQKRAGRHKGWISYTLGWTTRTIEGINVDNYGNPQPYYPKFD
                     QRHAANLVYGFDVTPRWTLNARYAFASGQGYTPVIGRYSISDPIFVSEQLYRDRLNSR
                     RLPYYSRLD"
     assembly_gap    5966..6065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6066..6959
                     /locus_tag="PLT86_05350"
     CDS             <6066..6959
                     /locus_tag="PLT86_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOT36069.1"
                     /translation="LTVAGCGLDPVSPAPREQLIIQAFLSPGAPVDNIRLTRTMDPAT
                     YYEGIAQTPVSGAVVVLRHGVSVDTLPELATGIYGNPSLIVQAGQTYSIAVTHGVHSL
                     AAETTVPTPVTVSAQTNGAPSRYVPSADTLVFPREYAYPDSFPDRATISPKPFKIFWT
                     PSVGIEGYQIAITARDTSGTGLLRLGDYNDWKAGDYRNARKRQREVTLTYPAFPDSLS
                     SDLFWVGFKYQGRYDIIVFAMDRNFYNYFMSSGGGNSGADYDTGVTNYVRGGLGVFGS
                     YAADTVRTFIKPEWLPSAERP"
     gene            complement(7008..8036)
                     /locus_tag="PLT86_05355"
     CDS             complement(7008..8036)
                     /locus_tag="PLT86_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOT36070.1"
                     /translation="MAVPRTVSEERLLAIICHLVERAGVPATDARRCARILVTADLRG
                     IPSHGVRMLPGKIHRVLGGAFNPNPRITKVAEIGSITVLDADRAIGMVAGAVAMETAI
                     SLARGNGISWVSVRNSNHFGAAGSYVMMAVEEGMVGFCFSNCGPIMAVYGTKSRCIGN
                     NPMAIGAPHRAFPVVLDMALSISSFGRIHTMKRNGDPVPDEWYAVPPEPGKDAVLRAM
                     GGPKGSGLAIMVEILTSVVAGGAIPWDMRPFKSTEKNDGTTHTMIALDVRRLVPEDQY
                     EETLDRFVEQLKTAERVEGVNEVILPGELEWRSTLKKRAEGIPLDADVVESLDNVAAF
                     LGTAVPWE"
     gene            8384..10183
                     /locus_tag="PLT86_05360"
     CDS             8384..10183
                     /locus_tag="PLT86_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36071.1"
                     /translation="MATITWSVFVTAPHITRYGRTAESRARLIKDVLDIGAKKVFLEV
                     WRGTVETDPDILRMLRDECKAAGLEVATGIMPVRGRDITLEGIPQWDSDTAIGGNSRW
                     GADICYSRPWSREALRKAMQTGAELFDEFIIDDALCTQCTCPRCREAKGDRTWTQFRR
                     DLLLEVCRETLVGTCRKVNPNIKMILKFPQWYDQLATFGYDTARQPAEFDATWIGTET
                     RDPDTPDFGFTPQYEACFNVRWHKAAEPNLEGAWFDYIECDTDLYVEQAYQSLLGGVR
                     NLTLFCYGDPLFRGKGHLLAALKEEQPRLEKLASRLDGQQPIGITAIRPHSPAPKNDG
                     YIFDGLGMLGMPLVPRADFPELLPPTVLLTDHVAGSPELTPFVSEVVNSGGTVFFTAS
                     LLAEHDDDEELKTLAGFGGDGWSQRNHWFSSHFMVGDRAVEASAPVEFRFDLRPSTAK
                     VLASVSGVAHHRAERIPVVTRQVHVSGGAVVVLNVFGASPADYRMDENLNVPIVLQMQ
                     NYPEPVVNVIQEQVALATGQAFLAPARVGYYPYISGDVVLENFTDEKVFAVPVGEQIP
                     LDARELLGVAGIEEKDGQRGVLLPPRSVALIGR"
     gene            10281..11510
                     /gene="coaBC"
                     /locus_tag="PLT86_05365"
     CDS             10281..11510
                     /gene="coaBC"
                     /locus_tag="PLT86_05365"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOT36072.1"
                     /translation="MLRGRRIVIGVTGGIACYKTCELVRLCVKAGAEVKVVMTEHATE
                     FVAPLTFETLSGHDVVVGMYGRNRAMSTEHIAMARWAEIIVIAPATANILAKAAHGIA
                     DDFLSTMIIATYSPVMFVPAMNTAMLKAPATQRNIPLLLESGYHITPTGKGELADGEV
                     GEGRMIEPREIMHHLVRALPRSGILAGKRVLVTAGPTEEPVDPVRVFTNRSSGRMGVA
                     LAEIAAGMGAHVTFVHGPLAVPQPIGADSMAVGTAQQMMDAVLTRFPETNVLVMAAAV
                     ADYRPKTVADQKIKKTTDTLTIEMERTPDILASVARKKKPGQIVVGFAVETEAEKADK
                     RALERLQKKSLDMIALNVIGEKGVGFGTDTNRVTLFCRDGSKSELPLMEKREIARIMW
                     ERIAALQGTGPQTKNQA"
     gene            11515..12396
                     /locus_tag="PLT86_05370"
     CDS             11515..12396
                     /locus_tag="PLT86_05370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT36073.1"
                     /translation="MDRAESRRETLQLLRRFVEERRELGDGFWPRPEGAAIPAKQGTR
                     AAPPTAARPPVSAERFLIPEKKPEHETAYHPATEQPAIAGESDLAGRLFASAPEGTVP
                     DAASLEEFRARISECRKCPLGATRTHFVFGSGNPNADLMFVGEAPGEEEDLQGQPFVG
                     RAGQLLTKMIEAMKLRREDVYIANVLKCRPPNNRTPLPSEMEQCKPYLDRQIALIRPK
                     IICALGRVAAQALLNTGEGLNRLRGKFHDYRGVKVLVTYHPAALLRNPQWKPLSWDDL
                     QKARYELDGTILRPKKA"
     gene            12409..13848
                     /gene="dnaB"
                     /locus_tag="PLT86_05375"
     CDS             12409..13848
                     /gene="dnaB"
                     /locus_tag="PLT86_05375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT36074.1"
                     /translation="MAGRNEGPAQEAMEELASAGAERVLPHSILLEQQVLGAMMLNRQ
                     AVSKVVSMLDRNAFYRGRHRLVFDACVRLYEADQPADLPLVIQELTRKGELAEAGGAE
                     YVADIAASVGTSTNVEYHAQQILELAVRRRTIALSSELINSAYDTTGTIYDNLERFEN
                     DLFKLAENRWREGFSHIEPIMHRTYERIEKVSTRKGEVTGVPSGFAALDEMTAGFQKS
                     ELIIVAARPGMGKTALVLNMARNAALQHKLPIGIFSLEMSAESVAQRLLCAEAHVDGQ
                     LVRRGRLNEHMWNRLSKAVTTLANAPIYIDDSAALSPLEVRAKARRLVHEHSVQLIIV
                     DYLQLMRVPGHREGRAQEVAVISQALKALAKDLDIPIIACSQVTREVEKRGEFRPRLS
                     DLRESGSIEQDADVVIFVHRPEYYKITHDEQGRNVEGLAEILLEKQRNGPTGKIELRW
                     TDKFGLFETYRRDDDEFATADVEEDGELY"
     gene            13848..14642
                     /locus_tag="PLT86_05380"
     CDS             13848..14642
                     /locus_tag="PLT86_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT36075.1"
                     /translation="MRGADLLSLRFARGTGHLVLASVRQVGRFVLFASEVVRALPRIF
                     QNPRLTLEQMVRMGTDSIPLVFTVSIFAGATTAWQGKYQLEGILPTAAIGTAVAKSLI
                     LELGPVLTALVIAGRVGSSIAAELATMRVTEQIDALVTMSISPARYLIAPRVVAGFVM
                     VPVLVVMSYFFGISGGYVTAVSLGGLNGARFLEGLRLFFYVRDVAVGLLKAFVFGAST
                     SFLGCYFGYYARGGAEGVGQAAIRAFVSSSVVILGADFLIASIAFM"
     gene            14679..15935
                     /locus_tag="PLT86_05385"
     CDS             14679..15935
                     /locus_tag="PLT86_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT36076.1"
                     /translation="MFSRDDCRRNLRWYILFRMSLDFMMWGPTWVLYMRDELGFSFTE
                     IMLFDFFFQTAIVLLEVPTGMFADIIGRRWALRGGAFSLFLAMGMWGAGWNAWWWISI
                     AWAFWAIAATLINGADASLVYETLRADGRGYEFGKVLGRISSLSMLGMVAASVLGGWM
                     AEYGYRVPILVHTALLAFTVFAAFRLREPPRAERLVHATVRNIARSIRALLKRSHRFR
                     YLLMYSALIQVVEIMAVVYQQPLLVSLGFPIKQLGVFYAGAILIASVGPIAMTWASDR
                     FGIARTLGLGALGTTIACLGLFWLPGLTIVIPFLVLRFFADGVRPVTIEGMNRLADPD
                     DRATVLSLRSICISSVAGPMEVLSGWWADRVPIRRVYLVCGALLPLFSVWLGWAWKRH
                     PEPMPKDVQAVPPVPRLGGDPSLHSE"
BASE COUNT         3355 a         5100 c         5053 g         3261 t
ORIGIN      
        1 gatctctgct gattgccgtg agggcgctgc ctgcggaaag gtctgcgtac agattggggt
       61 atttgtcgaa caggggacgc aacctgccgc ccggcttgac agggcctttc ggatatgcgt
      121 tggggtccga atcggcatcg ccggacacct ctcgccagaa gcccggggca tggcccagga
      181 agacagtgtc ggggcattgc ctgagcgccc gctcaaggtt ggaaatgtcc cccgcatacc
      241 accactgaac aggcttcgtg gggtcatcgg gtggcagaca ggggacgtca atgtggaggg
      301 tgaccgggag accgagttcg ccgcatacgc ggtagaaccg tatgcagtcg gggtcgtcga
      361 acagcgtgcg gaatttgtgc tcaccggaga cccgtacgcc gtgtttcacg gcagttttca
      421 gcttttcgat cgccatcggc gatttcggat ccggcgcgta ccccgcgacg aaccgcttcg
      481 ggtaccgttc taccgtatcc cagaccgctt cgatgggcat cccgatgtaa ggtgccatcc
      541 acgtgcggtc gtaactgtgg tcgtacatgc cttccgcgac ttcccacgtg agcaaccagg
      601 ccacatcgat cccgtgctca tccatgttcg cgacgagttt gtccgggtcg tacccgtacc
      661 accacggatg cgcgtgtgcg tcaacaatca taccggtacc tgctggtgaa aggattgtcc
      721 tctgcctcgc tgcagcgttt acgataatcc gaagagtatg atgccgggga caaggctgcg
      781 cagggatcac acgcttcaca tcgaacttct gacggagata cgcgtgaaat ccaatctgtc
      841 gcagagcaag ttctttcgat gcaatgtagt gtagccacgc tctgtcaccg aatcagctcc
      901 acgaagaacg cacgcgagaa gcagtgaagt gcgttgccgg ctcagcccca tggtagatat
      961 cttttgacgc agtccctttc cggcggacat ccacattcga aatgttgctg tcttgcctcg
     1021 ttccgtttga gtgattgcaa tcgcgggtca agacgcgaca gattggtctc acgccagcgc
     1081 atcccggagc tcgctcgaga aatgatcgga caacacgtgt ggttatggct cgttccaatg
     1141 ggctttgttc tgggtgcact tgggacgctg atcggcgcag gcggaggttt cgttcttgtt
     1201 cccgtcctgc ttttgatgta cccccggaac agccccgaaa ctattacaag catctccctg
     1261 gcggtcgtgt tcttcaatgc gttgtcgggt tcggtggcct acgggaagat ggggagaatt
     1321 gatttcagat cgggagctct gtttgccgca gccaccattc cgggagccat cctcggggcc
     1381 ttcaccaccg acgtcatacc ccgccggact ttcaatggcg tgttcggggt gctgatggtt
     1441 gcggcggccg cctacctcat tctccagcga gacaaaggtc ccggccacgt gggaagcgga
     1501 gacgcgcaca aaaccgtccg acgcattgtt gaaaaggatg gtaccgtcca cacgtactcg
     1561 ttcagtcttc gcgccggaat gttgtggagt actctggtgg ggtatatctc gagcctcctc
     1621 gggataggcg gggggatcgt ccacgtaccg gttctcgcgc gcctcctcaa tttccccgtc
     1681 cacatcgcca cggcaacgtc gcacttcgtc cttgcgatca tggcgctgac gggaaccgtc
     1741 gttcatatcg caaacggttc gtttgcacac ggtggcttct ggcgcacgct ctacctcggt
     1801 atcggcgttc ttttgggcgc gcaactgggg gcgtcgctct caaaccgtgt gaagggcgtg
     1861 tggatcatac gcagtcttgc tatcgcgctc ggactggtag gcgtgcgcat cctcctgatg
     1921 gcctttgagt cctgacccgc gcccggacac tctcacgcac ggttatcgct ccagaacccg
     1981 cccgcgttcg ggccacccgt tacccacgct ctgcctgccg ccgcgacatc cacccgagac
     2041 gaatcaccat gaggcaaagg aacaccggaa agacaatctc tttgagccag gatgatggca
     2101 ttgaatgggc cagcgctact cccgccagca cgcccacgca tccgggaagg gagagcgcaa
     2161 ctacggaacg ccagaggaca tttccccggc gggcgtggac gtatgcgcca ctcaaagagg
     2221 tcgggatgat catggccaga gacaccacct gaatcacgcg gaaatcgata tccgctatga
     2281 gtagcgccat accgggaaca accactattc cacccccgat ccccaccagg acggaaagaa
     2341 gaccggcgag cgctcccaca accaacaccc acgcaaccca gtgaactgga tcctcgagca
     2401 ggatgatccc ccgggccgga ccgagacctc ccagattcgc cattttcgcg gctgtaagca
     2461 gcaggaccac cataaacaca tggcgcaacg ccctgtccga gatacgatcg tggacctttc
     2521 ctccgagcca tacgcctccc tgcgcaccca ccgcgagtac tgccacaaga agccagggca
     2581 gtgtggtgcc tctctgcagt tctcgcgcaa cgccaatgaa ggaaatcaac aggaccgtta
     2641 cgatggagat ggcaaccgct cgacggatcg gaacaccgag gaaacctgtc agcaggggca
     2701 caatcagaac gcctccaccg atcccgagaa gcgcgcttgc cagaccgacc agtacgccga
     2761 tgccgagata tgctgccaga taccggcccg agggccagac atcagactcc acccacggtt
     2821 tttcctgtgt catccagcct ccgccaatca attcgggtac cgctctcgca cggaatccag
     2881 agtccgacta ttcaccgggt caacattctg tccgtccgtc gccagccgct cgtgcctccg
     2941 ccagaatctg cacgccgccg cgcgcaacaa ccaccgcgat tgccaggccg atcaccagat
     3001 cggggtaccg agaaccggtt attccaacga gaatccccgc tgcgatgact cccacgttcg
     3061 cgatcacatc gttcttcgaa aagatccacg acgctttcat gtggacctcg cccagacggt
     3121 gacgataaat gagcatcatg caggtaacgt ttgccaccag cgccacgagg ccaatcgcca
     3181 ccatcgcact gctgacagga tcgcttccga acacgaatcg tcgtgcgacc tcaacggacg
     3241 cgcctgctcc caggagaatc tgcgagaagc cgctcagtgc ggcggccctt gccttcagtg
     3301 cggcactcct gccaacggcg tagagactga tcgcgtagac agaagcgtcc gcaaacatgt
     3361 ccagtgaatc cgatataaga cccgttgatt gcgcgaccac gccaaagccg aactcgagca
     3421 cgaacatcaa tgcgttgatc gcaagaacgg taacaagggt ccggtgttct ccccgattac
     3481 tggcctcgac gtcacatccg caattcgcca caatcgtact ttccggaagg ttgttctgac
     3541 gagattgccc tccacctaat ctggcacaga acatcaaccg gttcccaccc tcctgatacc
     3601 cccgcagaaa aaacgtgcaa accgctcatt ttctggaacc ggtcacgcaa gttcaccggt
     3661 ataggaaggt tgagatgaca gacggcaccc cgcgtgcgaa cccggaagac tcattcttca
     3721 ccacgaatgg atagagaatg ccacaacgtt tcgttcggat tgccgcgnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt cttcaccacg aatggataga gaatgccaca
     3901 acgtttcgtt cggattgccg cgctcgttgc gtcgctcgcc gccgtgcttg ttccacgggc
     3961 cgctgaggcg gcaaccgtaa gcgggtatgt tcgagacaag tcctcgggcg agagcctccc
     4021 ttacgttcag gttttcttcg aggatttgcg cacaggcgcc atcacgaacc tggcggggta
     4081 ctatgccatc ccgaacgtgc ctccgggcaa ccatctgctt cgagttgcct tcctcgggta
     4141 tcaggattac acgaaggaaa tcaccgtcaa cggtggtgtg ctggtccaca atattgagct
     4201 tgccgaagag gcaatagagg tcgccgcggt tcaggtcgag gctgaacggg taaccgcacc
     4261 ctctctcacc atcgcaccga gccggacgac gctccggatg ccggatttcc gcaccgcacc
     4321 cgcgcttgca gaaccggatc caattcgcgt ggtgcagacg ttgccgggtg ttctgtcgct
     4381 ttccgatttt tccgtgggcc tctacgtgcg cggcagcaca ccggaccaga atcttcttct
     4441 ccttgatggc gtctcgcttt acaacgtttc tcacctgttc ggcctcttct ccgcgtttcc
     4501 ggcagatgcc gtgaaatcca gcgaactgct gaaaggcggg ttcccggctc agtacggtgg
     4561 ccggctgtcg tcggtgctga cgatcaacac cgatgaaggc aacaagcgca caatggtagg
     4621 gaaaggcggc gtaagcctca tcagctcacg cctcaccatg gagggccctg tcgcaaaggg
     4681 ttcgtggctt gtctcaggaa gaagaacgta tctggagcct atcctgaagc tggcctcccg
     4741 atggaatggc acgttcggca acctcggtta caatttctac gacgtccagg gaaaaacgca
     4801 ccaggtgttc tcacacgagc accagctctc cattgccggc tatgccgggg acgacaacct
     4861 gcgtttctcc gatcagacgt tcgattccca gttgctctgg ggaaaccggt ccctttccgc
     4921 aacgtggtcg tatgttccgg ttgaccggct gtttttccgc accaccggtg ccatcgcccg
     4981 gtatcgaagc cgattgcagc tcggaattga agacttcggg gttgtcgaac gaaatcgctt
     5041 gttcgactgg acggtcaaaa gcgatgctac ggtcttcctg actgagcaca acaccgtcga
     5101 gacggggttc gaattcatct gccacacgat gaattacacc gcggatttcg acaaacagac
     5161 atacaactcg ttttcgataa gaacccacca cgccagtgcc tacgcgcaga caaccctccg
     5221 tccgtgggcg tttctctccg ttcagccggg cgtgagagcc aactacttcg acaacggcgg
     5281 ttacgtggat atcgacccgc gcttctccgc gcgctaccag atcggcgaga acacctatct
     5341 caaaggggcg gtcggcaggt acagccagta cctcttccgt gttgcgcgcg agtttcaggg
     5401 gatcaacttc ctgtccgatc tctggttcac ggcggattcc accgccgagc cgagccatgc
     5461 gtggcagtac gtggcggggt tggaaacgaa actgaccccg gatatcgatc tgacgctgga
     5521 ggggtattac aaggacttcg aaatgctggc ggagttcaac agcgatgctc agacaccgat
     5581 gtcggcaggc cagatgctgc ggcgagggga tggcgaagcg tacgggctgg agtttggtct
     5641 tcagaagcgc gcgggaaggc acaaaggatg gatttcttac acgctgggat ggacaacccg
     5701 caccatagaa ggcatcaacg tggataatta cggcaacccg cagccctatt accccaaatt
     5761 cgaccagcgg cacgccgcaa atctcgtgta cggtttcgac gtcaccccga gatggacgct
     5821 caacgcacgg tacgcgttcg catccgggca gggatacact ccggtcatcg gacgctactc
     5881 aatctcggac cccatcttcg tgagcgagca gctctaccgg gatcgtctga acagcagacg
     5941 ccttccctac tattcgcggc tggatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnncttac ggttgcaggg tgcggacttg accccgtcag tcccgcgccg agggaacagc
     6121 tcattattca ggccttcctt tcccccggtg caccggtgga taacatacgc ctcacgcgga
     6181 cgatggaccc ggcaacctat tacgagggaa tcgctcagac acctgtttcg ggcgctgtcg
     6241 ttgtcctccg tcacggagta tcggtagata cacttccaga acttgctacc gggatctacg
     6301 ggaacccttc gctgatcgtt caggccggcc agacgtattc gatcgccgtt acgcacgggg
     6361 tccactcgct cgccgcagag actaccgttc ccacgcctgt caccgtctca gcgcagacaa
     6421 acggcgcgcc ctcgcgctac gtcccgtccg cagatacact ggtgtttccc agggagtacg
     6481 cgtatccgga cagcttccct gaccgcgcca caatttcgcc caaacccttc aaaatcttct
     6541 ggacgccctc cgtcggaatt gaggggtacc agattgcaat caccgcacgg gataccagcg
     6601 gcacggggct gttgcggctg ggcgactaca acgattggaa agcaggcgat taccgcaacg
     6661 cccgcaaaag gcagcgcgag gtgacactca cctaccccgc gttccccgat tccctgagca
     6721 gcgatttgtt ctgggtcggg ttcaaatacc agggtaggta cgacatcatc gtgtttgcca
     6781 tggatcggaa tttctacaac tacttcatgt cctcaggggg aggcaactcg ggcgcggatt
     6841 acgacaccgg cgtcaccaat tacgtgagag ggggcctcgg ggtgttcggt tcctacgccg
     6901 cggatacagt gcggactttc ataaaacctg agtggctgcc gtcggcggag cggccctgaa
     6961 caacaaagca ggggcccaaa agattaccgg acatggagcc gcgttgtcta ttcccacggc
     7021 acagccgttc caaggaaagc tgcaacgttg tccagcgatt ccaccacgtc cgcatcaaga
     7081 gggattcctt ccgctcgttt cttcagcgta gaacgccatt ccagctctcc cggcaggatt
     7141 acttcgttca ccccctccac gcgttccgca gttttcagtt gctccacgaa ccggtcaaga
     7201 gtctcctcgt actggtcctc cggaaccagc cgccgcacat cgagcgcaat catcgtgtga
     7261 gtcgttccgt cgtttttctc agtgcttttg aacgggcgca tatcccacgg gatcgctccc
     7321 cctgcgacca cgctggtaag aatctccacc atgatggcaa ggccgctccc tttcggccct
     7381 cccatcgccc gtaacaccgc atccttgccg ggttctggcg gtacggcgta ccattcgtcg
     7441 ggcacaggat ctccgtttcg tttcatcgtg tggatccgtc cgaacgaaga aatgctcagc
     7501 gccatgtcga gaacaacggg aaaggccctg tggggagctc ctatggccat cgggttgttg
     7561 ccgatgcagc gcgatttcgt tccgtatacc gccatgatgg ggccgcaatt ggaaaagcag
     7621 aacccgacca tcccttcctc gaccgccatc attacatagc tgcccgcggc gccgaagtga
     7681 tttgagttgc gcacgctcac ccaggagatg ccgttccccc gcgcgagcga aatcgccgtc
     7741 tccattgcta ccgcgcccgc caccatccct attgcccggt cggcatcaag aactgtaatt
     7801 gacccgattt ccgcgacttt cgtgatccgt gggttcgggt tgaacgctcc ccccagcacg
     7861 cgatggatct tgccgggaag catccgcacg ccgtgcgacg ggataccccg aaggtccgct
     7921 gtgacgagaa ttcgggcaca ccggcgcgca tcagtggcag gcacgccagc acgttctacc
     7981 agatgacaaa tgatcgcaag caggcgctct tcggacacgg ttcggggcac tgccataacg
     8041 cattccctca cagacaggcg tggcgttgaa cgggagttct gttccaagat acccggcccc
     8101 ctttcctgcg cgcaatgacg gcaggctttc ggggttcctg cggcgggatt gaacgcggat
     8161 ggtgcaggcg tttgcctgtc tatgccttgg gaacgaccgg ccctcgtgtg ccagtctccg
     8221 ggtgcctctc agtagagtgt cgaagggccc gacgcaggta cagcgattcc gctgacgcca
     8281 ccgtgttgcc cgcctccctg accttcgctc ccgaacaggg tagtcattat cattcacaat
     8341 gtcccttcgg aatccactgt caggaaaact cgcgaggatt atcatggcaa caatcacctg
     8401 gtcggtattc gtcactgccc ctcacatcac ccggtacggt cggactgcgg aaagccgcgc
     8461 acggctgatc aaggatgttc tcgatatagg cgcgaaaaag gtctttctgg aggtctggcg
     8521 cggcactgtc gaaactgacc cggacatcct gcgaatgctg cgcgacgagt gcaaagcggc
     8581 tggactggaa gtggccaccg gcatcatgcc ggtccgtggc cgcgacatca cactggaagg
     8641 tatcccgcag tgggatagcg acaccgcaat cggcggaaac agccgctggg gcgccgacat
     8701 ctgctacagc cggccgtgga gccgcgaggc gcttcggaag gcaatgcaga ccggtgccga
     8761 gttgttcgac gaattcatca ttgacgacgc cctctgcaca cagtgcacgt gtccgcgctg
     8821 ccgcgaagcg aagggcgacc gcacatggac tcagttccgg cgcgatctgt tgttggaggt
     8881 gtgcagagaa actctcgtcg gcacctgcag gaaagtgaat ccgaacataa agatgatcct
     8941 caaattcccg cagtggtatg atcagcttgc taccttcggg tacgatacgg ctcgccagcc
     9001 cgccgaattt gatgccacct ggatcggcac ggaaacacgc gacccggaca ctcccgattt
     9061 tggtttcacg ccccagtacg aagcttgctt caacgtgcgc tggcacaaag cggccgaacc
     9121 gaatctcgag ggcgcgtggt tcgactacat cgagtgcgac accgaccttt acgtcgagca
     9181 ggcataccag tcgctcctcg gaggcgtgcg aaatctgacg cttttctgct atggcgaccc
     9241 gcttttccgc gggaagggac acctcctggc ggccctgaag gaagagcaac cacggctgga
     9301 aaagcttgcg tcgcgactgg atggccagca accgatcggc atcaccgcca ttcgcccgca
     9361 cagccccgca ccgaaaaacg acgggtacat cttcgacggg ctcgggatgc taggaatgcc
     9421 gctggtgccg cgggccgatt tcccggaact tctcccgccc accgtactcc ttactgacca
     9481 tgtcgcaggg agcccggagc tcaccccgtt cgtgtccgag gttgtcaatt ccggagggac
     9541 cgtcttcttt accgcgtcgt tgctcgcgga acacgatgac gacgaggaat tgaaaacgct
     9601 cgccggcttc ggcggcgatg gctggtccca gagaaatcat tggttcagct cccacttcat
     9661 ggtaggcgac agggccgtgg aggcctccgc gccggttgag ttccggttcg acctcaggcc
     9721 aagcaccgcg aaggtgctgg cctccgtcag cggggtcgca catcatcgcg cggaacgcat
     9781 tcccgtggtt acgcgccagg tgcacgtctc cggtggtgcc gttgtggtgc tgaacgtgtt
     9841 cggcgcatct cccgcggatt accggatgga cgagaacctg aacgtgccga tcgtgcttca
     9901 gatgcagaac tatccggaac ccgtcgtgaa cgttattcag gagcaggttg cgctggccac
     9961 ggggcaggcc ttcctcgctc cggcccgggt ggggtactac ccgtacatca gcggcgatgt
    10021 cgtactggag aacttcacgg acgaaaaagt gttcgcggtg ccggtgggcg aacagatccc
    10081 gctggatgcg cgggaattgc tgggagttgc cggcatcgag gagaaggacg gacagcgtgg
    10141 tgtccttctg ccgccgcgtt ctgtggctct gatcgggagg taactccgcg tgttcttgcg
    10201 acgggaacgg ggggtgcgcg gcggcgtatc ccccgaatgt tccgtcgggt gcgcaggggg
    10261 ttggaaggag gaaccgggac atgcttcggg ggagacggat tgtcatcggg gtgacgggcg
    10321 gtatcgcctg ttacaaaacg tgcgaacttg tgcgtctctg cgtgaaggcg ggcgcggaag
    10381 tcaaggtagt catgacggag cacgcgacgg aattcgttgc accgctcaca ttcgaaacct
    10441 tgtccggcca cgacgtcgtc gtgggtatgt acgggcgcaa ccgcgcgatg tccacggaac
    10501 acattgctat ggcacggtgg gccgaaatca tagtcattgc gccggcaaca gcgaacatcc
    10561 tggcgaaagc ggcacacggt atcgcggatg actttctcag cacgatgatc attgccacgt
    10621 attcgccggt catgtttgtg cccgcgatga acaccgccat gctgaaggcg cccgccactc
    10681 agcggaacat ccctctgctc ctggagtcag gttaccacat cactccgacc gggaaaggtg
    10741 agctggccga cggcgaagta ggcgaagggc ggatgatcga gccgcgtgag atcatgcatc
    10801 atctcgtgag ggcgcttccc cgctccggca ttctggcagg aaagcgggtg ctggtgacgg
    10861 cgggaccgac agaagagccc gtcgaccctg tgcgcgtgtt caccaaccgg tcatcgggaa
    10921 gaatgggcgt cgcactcgcc gaaatcgccg cggggatggg ggcacacgta acgtttgtcc
    10981 atggcccgct ggcggtacct caaccgattg gtgccgacag tatggcagtg ggaaccgctc
    11041 aacagatgat ggacgcggtt ctcaccagat tcccggaaac gaatgtgctc gtaatggcgg
    11101 ctgcagtggc tgattaccgc ccgaaaacgg ttgccgacca gaaaatcaag aaaacgaccg
    11161 acaccctgac aatcgaaatg gagcgaactc cggacattct cgcatcggtt gccaggaaga
    11221 agaaaccggg gcagattgtg gtcggcttcg ccgtggaaac cgaggccgaa aaggcggata
    11281 agcgggcgct ggaacgcctg cagaagaagt ccctcgacat gatcgcactg aatgtcatcg
    11341 gcgagaaagg cgtaggcttc ggcaccgata cgaacagagt gacattgttc tgccgggacg
    11401 ggtcgaaatc cgaactcccg ttgatggaaa agcgagagat cgcgcggata atgtgggaac
    11461 ggatcgcggc gcttcagggc acgggtccgc agacgaaaaa ccaggcgtaa gttcatggat
    11521 cgcgccgaat cacgcagaga gacgttgcag ttgctccgcc gcttcgtgga ggaacgccgc
    11581 gaactcgggg acggattctg gcccagaccc gaaggtgcag cgatccctgc aaagcaggga
    11641 acacgtgctg ctccgccgac tgcggctcga ccgccggtat ccgcggagcg tttcctgata
    11701 ccggagaaaa agccggaaca cgagaccgcg taccaccctg cgacagaaca gccggcgatc
    11761 gcaggagaga gcgacctcgc cggccgcctg ttcgcttctg cgccagaggg cacggttccg
    11821 gatgcagcat cgctggagga gttccgcgcg cgcatcagtg aatgccgcaa atgcccgctt
    11881 ggcgcaacgc gtacgcattt cgtattcgga tcgggcaacc cgaatgccga cctcatgttt
    11941 gtaggggagg cacccggaga agaagaagac cttcaggggc aaccatttgt cgggcgtgcg
    12001 gggcaattac tgacaaaaat gattgaagcg atgaagctca gacgggagga cgtgtatatc
    12061 gccaacgtgc tgaagtgccg ccctccgaac aatcggactc ctctgccgag cgagatggag
    12121 cagtgcaagc cctatctgga caggcagatc gcgttgatcc gcccgaagat catctgtgca
    12181 ctgggccggg tggcggcgca ggcgttgctg aacaccggcg aggggttgaa ccgtctgagg
    12241 ggtaagttcc acgattaccg cggcgtcaaa gtgctggtca cgtaccatcc cgccgcattg
    12301 ctccgaaacc cgcagtggaa gccgctgtcg tgggacgacc tgcagaaagc acgatacgaa
    12361 ctcgacggga ccattctgcg gccgaagaag gcatgaggag aggagagtat ggcgggccgg
    12421 aatgaaggtc ctgcgcagga agcaatggaa gaactcgcga gcgcgggtgc ggagcgggta
    12481 ctgccccatt ccatcctgct cgaacagcag gttctcggcg cgatgatgct caaccggcag
    12541 gcggtgagca aggtcgtttc catgctggat cggaacgcat tttaccgggg ccggcaccgt
    12601 ctcgtgttcg atgcgtgtgt gcgcctgtac gaagccgatc agccggccga cctgccgctg
    12661 gtgattcagg aactgacacg gaaaggcgag cttgcggaag ccggcggcgc ggagtatgtg
    12721 gccgacatcg ccgccagcgt cggtacaagc acaaacgtgg aataccacgc gcaacagatt
    12781 ctggaactgg cggtccgccg gcgtacgatc gcgctttcca gcgaactgat caattccgcg
    12841 tacgatacca ccgggacaat ctacgacaac ctcgagaggt tcgaaaacga cctgttcaag
    12901 ctggcggaaa accgctggag ggagggattc tcccatatcg aaccgatcat gcaccggacg
    12961 tacgaacgga tcgagaaggt cagcacgcgc aagggagaag ttacgggggt tccgagcggg
    13021 tttgccgcgc ttgatgagat gaccgccgga tttcagaaga gcgaattgat catcgtcgcg
    13081 gcgcgtccgg gtatgggaaa aaccgcactc gtgctcaaca tggcgcggaa cgcggctctc
    13141 cagcacaaac tacccatcgg catcttctcg ctggaaatgt ccgccgaatc cgtggcccag
    13201 cggcttctct gcgcagaggc gcacgtggac ggccaactgg tgcgccgcgg cagactgaac
    13261 gagcacatgt ggaaccggct ttccaaagcc gttacgaccc ttgcgaacgc gccgatctac
    13321 attgacgaca gcgccgcgct ttcccctctg gaagtgcgcg caaaggcccg cagactcgtc
    13381 cacgaacaca gcgtccagtt gatcatcgtc gactacctgc agcttatgcg cgttcccggg
    13441 cacagggaag ggcgcgccca ggaagtcgcc gtcatctccc aggcactgaa agcgcttgcg
    13501 aaggatctgg acatcccgat catcgcctgc tctcaggtca cgcgcgaagt ggagaagcgc
    13561 ggggagttcc ggccgcgcct ttccgatctg cgcgagagtg gatccatcga acaggatgcg
    13621 gacgtcgtca tttttgtcca tcgccccgaa tactacaaga tcacgcacga tgaacagggc
    13681 cggaacgttg aaggactggc ggaaatcctg ctggagaaac agcgaaacgg cccgacgggg
    13741 aaaatcgagc tgcggtggac ggacaaattc ggcctttttg agacctaccg gcgtgacgac
    13801 gacgagttcg ccacggcaga cgtcgaagaa gacggggagc tttattgatg cggggggccg
    13861 atctgctctc acttcgtttc gcgcgcggca cagggcatct cgtgcttgcc tccgtgcgac
    13921 aggtgggaag gttcgtgctg tttgcctccg aagtcgtgcg tgcgctgccc agaatcttcc
    13981 agaatcctcg cctgacgctc gagcagatgg tgcgcatggg aacggattcc attccgctcg
    14041 tgtttacggt gtccatcttt gccggcgcga cgacggcatg gcaggggaag taccaacttg
    14101 aaggcatcct gcccaccgcg gcaatcggta cggcggtggc gaaaagcctc attcttgagc
    14161 tgggtccggt gctcacggcg cttgtcatcg ccgggcgcgt ggggtcttcc atcgccgcgg
    14221 aacttgccac gatgcgcgtg acagagcaga tagacgcact cgtcaccatg tccataagcc
    14281 ccgcgcggta cctcattgct ccccgcgtgg tcgccgggtt cgtgatggtt ccggtactgg
    14341 tcgttatgag ctatttcttc ggcatttcgg gcggctacgt caccgcggtg tcgctcggag
    14401 ggctgaacgg ggcaaggttt cttgaagggc tgcgcctgtt cttttacgtg cgtgacgtgg
    14461 cggtcgggct cctgaaagcg ttcgtgttcg gcgcatccac gtcgtttctc gggtgttact
    14521 tcgggtatta cgccagaggt ggagccgaag gcgtaggcca ggccgcgatc agggcgtttg
    14581 tgtcctcttc ggttgtcatt ctgggagctg atttcctcat cgcttcgatt gcattcatgt
    14641 aaccatcgtc tccacccctt ttccccctcg gaatacgcat gttctcccgc gatgactgcc
    14701 gccgcaacct caggtggtac atccttttcc gcatgtcgct cgacttcatg atgtggggcc
    14761 ccacctgggt gctctacatg cgcgacgaac tcgggttctc cttcaccgaa atcatgctgt
    14821 tcgacttctt cttccagacg gcaatcgtgc tgctggaagt gccgaccggc atgttcgccg
    14881 atatcatcgg ccgccggtgg gcactccgcg gcggcgcatt cagcctcttt ctcgccatgg
    14941 gaatgtgggg cgcgggctgg aacgcctggt ggtggatcag catcgcgtgg gcattctggg
    15001 caattgccgc caccctcatc aacggggcgg acgcgtcgct cgtgtacgaa accctgcgcg
    15061 cggacgggcg ggggtatgaa ttcgggaagg tcctcgggcg catttcgagc ctttcgatgc
    15121 ttggcatggt tgccgcgtcg gttctgggcg gatggatggc ggaatacggc taccgcgtgc
    15181 cgatcctcgt ccacaccgcg ctgctggcat tcaccgtgtt cgctgcgttc cgccttcggg
    15241 agccgccgcg cgccgagagg cttgtccacg cgacggtgcg caacattgcc cgttccatcc
    15301 gtgcactcct caaacgcagt caccggttcc ggtatctgct catgtactcg gcgctgatac
    15361 aggtggtgga aatcatggcc gtggtgtatc agcagccgct tctggtgtcg ctcggttttc
    15421 cgatcaagca actcggcgtg ttctacgcgg gtgcaattct cattgcgtcc gtcgggccga
    15481 tcgccatgac atgggccagc gaccgtttcg gaatcgcgcg cacgctgggg cttggcgcgc
    15541 tgggcaccac aatcgcctgt ctggggctgt tctggttgcc ggggcttacg atcgtcatcc
    15601 cgttcctggt cctccgtttt tttgccgacg gagtgcgccc ggtgaccatc gagggcatga
    15661 accggctggc ggacccggac gatcgggcga ccgtgctctc gttgcggagc atctgcatct
    15721 ccagcgttgc cgggccgatg gaagtgctgt cggggtggtg ggcggacagg gtgccgattc
    15781 gccgggtata cctcgtgtgc ggggcgctgc tgccgctctt ctccgtgtgg ctcggatggg
    15841 cgtggaaacg gcacccggag cctatgccga aggacgtgca ggcggtgccc ccggttcccc
    15901 ggctgggggg agacccgtcg cttcactcgg agtgacgaga gcggctaccg gtcacgacgg
    15961 caacgctgag tcgttacacg cgtgcccctc cacgcacgcc cgccctggag ggacccgctc
    16021 tgtcgggtcc tgcgccctgc gaggaggttg ggcgcgtggg ttttcccctg gattcccgcc
    16081 ttcgcgggaa tgacaggcac tacacgagca ttgcgtcatt tgtgcgtcgt attcccatcc
    16141 tcggggcgct ggcagatcca accttcacag aacacccacg acgaaaagcg tcgtcattcc
    16201 cgcgaaggcg ggaatccaga acacactgct gaagagattc ttcgctttgt ccgcgtggcg
    16261 gacgccgctc agaatgacgg ggaagggggc ggacggtaga tcagcggcag ggtgcaccga
    16321 cggtcgggcc gggtagttgc cgcgaggcct tggagtgcgg aggcgcgcct ccgctttgaa
    16381 agcgcaagca cgtttgcgca gtccagagtg agttgcgtgt ttgctcacgg ggtgcgcggt
    16441 ggcacttcga caagctcagt gaccaccgcg tgtcgtcgag ataacgcggc gcagagaatc
    16501 tacgtttcgt tcagacggac gagcggcggc aggtcgttgc gttcacgcca cccgaagacc
    16561 ggcggagcag gcggagggca cgacggagcg tgcccctcca cacatacccg ccttggaggg
    16621 acccgctctg tcgggtcctg cgccctgcga ggaggttggg cgctgggaga tccaaccctc
    16681 acagaatacc cacgaagaaa agcgccgtca ttcccgcgga agcgggaatc cagagcaccc
    16741 tgttgaagag attcttcgca ccgtccgcgc ggcggacgcc gctcagaatg acgggtaggg
    16801 gggagcagaa tcacggtccg ggggccgagg gaacacccga acaaagcctg cattggaggg
    16861 acccgctctg tcgggtccaa cgggcgtgcg cgggaatctc actttgccac ctacggcttc
    16921 gggtagctga tcgccagcat ggcctcgatg tcaagcggat caacgccga
//
LOCUS       DAOSOO010000050        16835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3437_length_16835_cov_5.558701, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000050 DAOSOO010000000
VERSION     DAOSOO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16835
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_16835_cov_5.558701"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLT86_05390"
     CDS             <1..943
                     /locus_tag="PLT86_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36077.1"
                     /translation="ILRAAHEAKNVRLAVVYDVDPERARSAAEKTGAKVAASYEEMLR
                     DDSLQGVILVVPNPLHRRMLEAAVDAGKHVYVEKPIANSVKDGMGMRRYAAAAGRILM
                     VGHNTRRAQTFRTVKHMIDRGELGTVVAMEANFSHAGGLGVPPDAWRYRRETCPAVPL
                     MQLGVHCVDTMNYFAGAPVRVSSFMSSRIMKQNDDVTVTLVEYENGVLATLHSHYVVA
                     GTNFVAAYGSEANVYAEWERLDKTTREPHGVVSTPFPRNDTQLEEISEFAACIESGAQ
                     PETDATAGILALAVVEAAIISHKEQRIVSLEEILQDG"
     gene            complement(955..2016)
                     /gene="amrS"
                     /locus_tag="PLT86_05395"
     CDS             complement(955..2016)
                     /gene="amrS"
                     /locus_tag="PLT86_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT36078.1"
                     /translation="MHEAMFYERLAGNSVRCQLCHQQCVIADRHHGLCGVRENHGGTL
                     YSVNYGRVIAAHVDPIEKKPLFHFCPGSRAFSVATAGCNFRCAFCQNWDISQASKGEN
                     RSIGGHYTEPQATVEAALRTSSRVIAFTYTEPTVFYEWALDVARAGAEKDLKTAFVTN
                     GYMKADPLDFIQPFLHAANVDLKAFRDESYRRVMGAALQPVLDTLIRMKRLGIWVEVT
                     TLVVTDLNDSEDELRDIARFISSELGPETPWHVSRFHPDFRYTNVPPTNPHVLKRAHE
                     LGQEEGLHYVYVGNLPGTDGENTRCHKCGNLLVNRIGFHVVENRIATGSTCPQCGERV
                     AGVGMEVVSQRGNSSAGTR"
     gene            complement(2039..2392)
                     /locus_tag="PLT86_05400"
     CDS             complement(2039..2392)
                     /locus_tag="PLT86_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT36079.1"
                     /translation="MDGREKPKYIVRTTEGLDEVRSTCGFRKSIFTEDDGAGFSVSLL
                     RISDSKKHYHRATWETYYVLEGEGELELDGEMIALSQGTAVLIRPGVRHTARGNVIAL
                     IMGAPPFRTDDMFFD"
     gene            complement(2400..2873)
                     /gene="smpB"
                     /locus_tag="PLT86_05405"
     CDS             complement(2400..2873)
                     /gene="smpB"
                     /locus_tag="PLT86_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT36080.1"
                     /translation="MPAGIKIITKNRKAFHDYSILETTEAGIALVGTEVKSLRLGNVN
                     LKDSYARVERGEVVLYGLHISPYDHGNRYNHDPERPRKLLLHRREIGRLKGRSEEKGL
                     TLVPLSLYFKGGRVKVELGLAKGKQIHDKRETLRRREIEMDMEQAIRRQSREQLE"
     gene            3025..3918
                     /locus_tag="PLT86_05410"
     CDS             3025..3918
                     /locus_tag="PLT86_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36081.1"
                     /translation="MPLLLGALMLLCASCSPATSDCLDTLSEIGSSPEIVAASRASVF
                     PDTSCGGLLFSIGTAEYAQASFIAARKAFEAATVFWPDSSKAWNGLGLANYRIRDYSA
                     AERAFKRSLRLAPRQIDVYSNLGLVFLAQARYIDATAAFIAALGINPGYTEARFNLAY
                     TYDLQGRPDLAEGEYYRVLRDHPDHPTARYGLAVLFRKAERFGEALAQLDSALATHPD
                     YADAGVERGFLFLRRNEYSRAAGDFRRVLDSHPENAQARYGLGLAYAFQRQWVAAHAQ
                     YESLAVADTTYARRLRRVLGN"
     gene            complement(3987..5267)
                     /gene="eno"
                     /locus_tag="PLT86_05415"
     CDS             complement(3987..5267)
                     /gene="eno"
                     /locus_tag="PLT86_05415"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT36082.1"
                     /translation="MSMIESVHARQILDSRGNPTVEVEVVLEDGSFGRAAVPSGASTG
                     AFEALELRDGEAAYGGKGVLKAVKNVNETIAPEIEGLDATDQVEIDELMIKLDGTPDK
                     SNLGANAILGVSLAVAKAAAETCGLPLYQYLGGPSARVLPVPMMNILNGGKHAVDSTD
                     LQEFMVMPVGAPTFSEALRWCAETYQALKKVLSAKKYNVSIGDEGGFAPSLKSNEEAI
                     EVILQAIEKAGYKPGDDIMIALDPAASEIFEDGKYVLAKEGRTLSAPEMVDFYERWVA
                     KYPIISIEDGMAEDDWEGWALLTERLTDKIQIVGDDVFVTNTERLQKGIDEEVANSIL
                     IKLNQIGSLTETLAAIEMAKCASYTAVVSHRSGETEDTTIADLVVATNAGQIKTGAPC
                     RTDRVAKYNQLIRIEEALGSVAKFAGRSAFYSIG"
     gene            complement(5419..6369)
                     /locus_tag="PLT86_05420"
     CDS             complement(5419..6369)
                     /locus_tag="PLT86_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36083.1"
                     /translation="MKTSVASNWLCCVVLAGFVCSCGSSFRFPAEVVRLVLPRTGSEI
                     VGEVQLPARRTFAPVPLVVLVDDGPPIDIDPRRTQANQIQQLQAALVSNGYAVWRPLR
                     DAWPSDSLVVRCPDKLTSQVLLGFLSARNIRGIDTARVVFLGFGQGGVIAALAAERSA
                     GRVHALGLVNTPARSVDRTLLSGTFRDSVNLRRLEQFFDDIWTDKYNDSDFVMNGRVE
                     CWRSWVVLSGEMTDRVARLSVPTLAVQGTADSLLPLLDIERFRRAIGRRQLSQVHTAV
                     GLRHDLRDEIPDPGQDKKAISPRFVSIVLEWLKLVAPPAG"
     gene            complement(6412..7704)
                     /locus_tag="PLT86_05425"
     CDS             complement(6412..7704)
                     /locus_tag="PLT86_05425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT36084.1"
                     /translation="MSVTVAVGTHWGDEGKAKVVDFLTEKNDIVVRFQGGANAGHTVI
                     ADGETWIFHQIPAGILHPGKVCVVGNGVVLDPLAFPEELDHVRGRGVSVEGRLWISDR
                     AHLVMPYHKQLEGIQEAARGTGAIGTTKRGIGPTYYDKISREHGLRAGDLLDAMGFRA
                     RLRKIAADKNEVFTRVYGAEPVNADAVADEYLGYAEQMRPFIADTATILHAAQNAGKA
                     ILCEGAQGTLLDIDHGTYPFVTSSNTSAGGACTGTGIGPTAISEVIGIVKAYTSRVGN
                     GPFPTEFECEMGNRMRELWNEYGATTRRPRRCGWLDGVILEYSARINGLTGLAVTALD
                     RLDALSELRICREYVLDGTPLHTVPARTEDFDRVKAVYDTLPGWETDTTSARCFEELP
                     ENARRYIRRIEEIAGVPVRYVGIGPERDQMIVVRSQTV"
     gene            7998..9269
                     /locus_tag="PLT86_05430"
     CDS             7998..9269
                     /locus_tag="PLT86_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOT36085.1"
                     /translation="MEQWPTLVIFAATYIGVAVGRVPGLQIDRTGIALMGAIGMVLVG
                     QITVKEALDAVDFPTILLLYALMVVSAQFRLGGFYTWVAGRVARLARRPVLLLAALIS
                     VTAVLSALVANDIVCLAFTPVLCIALQRAGLNPVPYLLALACASNIGSAATIIGNPQN
                     MLIGQVGRLDFDAFLFWCAPPSLAGLLVVFGVCLLSFPREFLADGQHHGAGSPNPPES
                     LEGPPDFGGELDAHQTLKGIIVTIVLVAAFFTSIPREVSGLAAAALLLASRKIHSRAI
                     LGFVDWHLITLFCALFIVVHGIEAGGFPAAAVAALKEYGISVGSLPVLGTVAVVLSNL
                     VSNVPAVMLLIGQLPQGNPVPWYVLALTSTLAGNLILVGSIANLIVVEQAEQQGVHVS
                     FAVHARVGVPVTLITLALTRLWIVLALWMTG"
     gene            complement(9315..9902)
                     /locus_tag="PLT86_05435"
     CDS             complement(9315..9902)
                     /locus_tag="PLT86_05435"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT36086.1"
                     /translation="MLRAARISDVAEMAQFINSFAQRNLMLPRPLNRMYENLRDYVVV
                     EEDGKVVGCGALHILWYDLAEIRSVAVREGFQHRGYGKAILNSLLDDARKLGVHQVFM
                     LVLPDGAMARLARKSGFREVSKDELPHKVWSDCLNCPKFTECDEIALIAEVGPKIESP
                     HQWSSVMSAYVRRGGDADSVAIPLDIPKPSPEKTR"
     gene            complement(9911..11290)
                     /gene="argH"
                     /locus_tag="PLT86_05440"
     CDS             complement(9911..11290)
                     /gene="argH"
                     /locus_tag="PLT86_05440"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT36087.1"
                     /translation="MTTGKLWDGRFSRATDQVMEEFNASIGFDIRLWEADIEGNLAQA
                     DALRQCGILTSEEWSAIVLGLQQVRQELAGGSYRPGPDTEDIHMAVERRLIEIVGPVG
                     GKIHTGRSRNDQVALDVRLYLRRAVGTLTELVSQLQAVVLKRAEAEVDAFLPGYTHLQ
                     PAQPVRLAHYLLAFFWMLDRDKGRLRDALRRADEMPLGSGALAGTAFPIDREFVARRL
                     GFARPTRNSMDAVSSRDHLTETVSAIAILMTNLSRLAEDWVVWSSPAFGFVELDDAYA
                     TGSSMMPQKKNPDSLELVRGKSGRVFGDLMALVTIQKGLAFTYGKDLQEDKEPLFDAL
                     ETADICLRVLKGVVETAVFNHERMRAAIEPGMYATDLADVLVRRGLPFRDAHRVVGEL
                     VGKAIARGCSLVELPWEEFAQASPLFKREDVSTLSPAASTDRRNLFGGTGRNAVVQQL
                     ALARASLKE"
     gene            complement(11301..12518)
                     /locus_tag="PLT86_05445"
     CDS             complement(11301..12518)
                     /locus_tag="PLT86_05445"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT36088.1"
                     /translation="MASEVKKVALAYSGGLDTSVIIPWLKETYGCEIVAVVADLGQGE
                     DELHGIEEKAIRSGASDCVVLDLKQQFIEGYIWPTLKAGAIYERKYLLGTSFARPIIA
                     KAQVQVARETGADALAHGATGKGNDQVRFEVTYAALAPDLKVIAAWRDPAWKIRSRED
                     ALDYAAAHNVPVTASRKSIYSRDRNIWHLSHEGGALEDPWQEPPRDVWVLTVHPEDAP
                     DVPRYVEIQFEAGIPVGINGVRKDSVSLLTELNALGAAHGVGRCDLVENRLVGMKSRG
                     VYETPGGTIIYAAHRELESLVLDRETLHYKDIIAQRYAELVYYGKWFTPLREAIDAFV
                     DATQRDMNGTVRMKLYKGSCTVAGVQSENSLYDEALATFGASSYQQSDATGFLKLFSL
                     PERTIAMLKQRKG"
     gene            complement(12556..13788)
                     /locus_tag="PLT86_05450"
     CDS             complement(12556..13788)
                     /locus_tag="PLT86_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT36089.1"
                     /translation="MHDDSQSASLPAGKGVSREVIRRSKEFLLQNYARADVVFERGEG
                     VYLFDAEGERYLDCVSGLAVNALGYGDADVLEALREQSRKLIHVSNLYYTIPAASLAE
                     ELVRHSSADRVFFCNSGTEANEACIKFARRWARTHYGENKTGLVAAFSSFHGRTMGSI
                     SLTGQEKYQAPFEPLVPGIRFARFNDIDHTVEQIDDSTAAVFLEPVQAEGGVHPATQQ
                     YLKAVRSACDRHNALLVFDEVQVGLGRTGRLFCHEHYGVEPEMLTLAKPLAGGLPIGA
                     ALLKQHVADAIQPGDHAATFGGGPLICAVALVVFRKIADPQFLEKVRNNATLFGGRLR
                     ALKEQYSAIREIRGMGMIWGVQFDEPVAPIVAEFRKRHILVCVAGPNVLRFLPPLVIG
                     EEELNHVADVFAEILEAR"
     gene            complement(13841..14605)
                     /gene="argB"
                     /locus_tag="PLT86_05455"
     CDS             complement(13841..14605)
                     /gene="argB"
                     /locus_tag="PLT86_05455"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT36090.1"
                     /translation="MALVKIGGSTLDSPGLIGELAFDLARLGAGRTILVHGGGKDINR
                     ALDRLGQPTTFVDGLRVTDAAAVETVEAVLSAQVNKRLVRALLSAGARAVGISGVDGA
                     LLQARPYGDGRLGYVGAIEKVDPSVLVALLNAGLLPVVSPISVGGDGLAYNVNADHAA
                     ADIAMAMKVDELVFVTDVPGVLVEGRTVPLLTGADAEKLIAIGQITGGMIPKVRSCVE
                     AVRRGVGRVHILAWDGAATISEHLAGVRSHGTVITD"
     gene            complement(14633..15661)
                     /gene="argC"
                     /locus_tag="PLT86_05460"
     CDS             complement(14633..15661)
                     /gene="argC"
                     /locus_tag="PLT86_05460"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT36091.1"
                     /translation="MKRQVSVTIFGASGYTGAGLYGLCLRHPGVNVAALSADRHAGER
                     LSAVFPRMAGLTDRVMVSVEEAVEQANEVAFLCLPHEAAMAVVPRLLDKGCKVFDLSA
                     DFRMDTPDSYAAWYKVSHTAPELLGVKVYGLPEFHRERIREARLIAVPGCYPTSVQIG
                     LAPLLKHRLIDPRTIIVDAKSGVSGAGRKLSQRTHYVETNENIAPYNVGRSHRHVGEI
                     EQELSKFAGEAVRVVFSPHLTPMNQGILSTMYVDLTHPLGTSELLDLLRSEYSGERFV
                     RVLPHLPETAFVQNTNYADVSVHVVPDTGRAIVLCAIDNLVKGAYGQALQCMNIACGF
                     EEECGLPV"
     gene            complement(15658..16125)
                     /gene="argR"
                     /locus_tag="PLT86_05465"
     CDS             complement(15658..16125)
                     /gene="argR"
                     /locus_tag="PLT86_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT36092.1"
                     /translation="MKTSQKLARQREIVRLVRAQTIRTQAELANALRERAFPVSQPTL
                     SKDIAELGLIKVPLPDSGYRYQLPTEGGVEHPAGRLQFALREFLFSWEAAGQLIVMKT
                     VAGHAAGVAWALDTEKWSDILGTIAGEDTVLVVCRTPADANHVLRQLQQVLEG"
     gene            16251..>16835
                     /locus_tag="PLT86_05470"
     CDS             16251..>16835
                     /locus_tag="PLT86_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT36093.1"
                     /translation="MRKLIIGIIRLARPVQWVKNVFVAAAPVYAGQIFVPEIFGKTSA
                     AVLLFCALSSGVYIINDYFDMARDREHPVKKNRPLPSGQVPVPIALAVGFALLAGSLF
                     GCAFVSPGLLAVAAAYVSVNGLYTLWWKQVVVLDIMCVASGFVFRVAAGGLAVGVPVR
                     PWILLCTLCLALLISLGKRRGEALLLGENAAKHRR"
BASE COUNT         3345 a         5330 c         4573 g         3587 t
ORIGIN      
        1 gatcctccgg gccgcacatg aagcgaaaaa cgtgaggctc gcggtcgtct atgatgtcga
       61 ccccgagagg gcacgatcgg cagccgagaa aaccggggcg aaggtggcag cgtcgtacga
      121 ggagatgctg cgcgatgact ccttgcaggg cgttatcctg gttgttccca accctctcca
      181 caggaggatg ctcgaagccg ccgtggacgc cggaaaacat gtatacgtgg agaagcccat
      241 cgcgaacagc gtgaaggacg gcatgggaat gcggcgctat gcggcggcag ccgggcggat
      301 cctgatggtg ggacacaata cgcgtcgcgc gcagacattt cgaacggtca agcacatgat
      361 cgaccgcgga gaactcggga cggtggttgc catggaggcg aacttctccc acgccggcgg
      421 cctgggtgtc ccgccggacg cgtggcgtta ccgccgggaa acctgtcccg ccgtaccttt
      481 gatgcagctc ggggttcact gcgtcgacac gatgaattac ttcgccgggg cgcccgtccg
      541 cgtcagttca ttcatgtcct ccagaatcat gaaacagaat gacgacgtga cggttaccct
      601 cgtggagtac gaaaacggtg tgctggcaac gctgcacagt cattatgtgg ttgccgggac
      661 gaacttcgtg gcggcgtacg gatcggaggc aaacgtatac gccgaatggg agcggcttga
      721 caagaccacg cgcgagcccc acggagttgt gtccacacca ttcccgagaa acgacacgca
      781 gctggaggaa atcagtgagt ttgccgcttg catagaatcc ggagcacagc cggaaaccga
      841 cgccacagcg ggcatacttg ctctcgccgt agttgaagct gcgatcatct cacacaagga
      901 acaacgcatc gtgtcgctgg aagagatttt gcaggacgga taagcggcac gaaatcaacg
      961 ggtaccggcg ctcgaattcc cccgctgaga gactacttcc atcccgacgc cggcaacgcg
     1021 ctctccgcat tgagggcagg tgctaccagt cgcgatccgg ttctccacga catggaatcc
     1081 gatccgattt accagaagat tgccgcactt gtggcaccgg gtgttctccc catccgttcc
     1141 cggcagatta cctacgtaca cgtaatgaag gccttcttct tgccccaact catgcgcccg
     1201 cttcagtaca tgcggattcg tgggtggcac gtttgtgtac cgaaaatccg gatggaatcg
     1261 gcttacgtgc cacggagttt ccgggccaag ttcacttgaa atgaagcgcg cgatgtctcg
     1321 aagctcgtcc tccgaatcat tcaaatccgt gaccacgagt gtggtaacct caacccagat
     1381 gcccagtctc ttcatacgaa tcagggtgtc gagcacgggt tgaagagcgg ctcccatcac
     1441 gcggcggtac gattcgtctc tgaacgcctt cagatccacg tttgccgcgt gaagaaacgg
     1501 ctgtatgaaa tcaagcggat ctgccttcat atacccattt gtgacgaacg cagtcttgag
     1561 gtccttctcc gcccccgccc gtgccacatc cagagcccat tcatagaaca ctgttggttc
     1621 cgtgtacgtg aaagcgatca cgcgagatga tgtgcgtagc gcggcctcca ctgtagcctg
     1681 cggttcggta taatggccac caatggaacg attctccccc ttgctcgcct gcgagatatc
     1741 ccagttctga cagaaggcac accgaaagtt gcatcctgcg gtcgccacag aaaatgcacg
     1801 ggacccgggg cagaaatgga acaacggctt cttttcgatg gggtcgacgt gcgccgcgat
     1861 cacgcggcca tagttgacgg aataaagggt gccaccgtgg ttttcacgga ctccgcataa
     1921 tccgtgatgc cggtccgcta tcacacactg ctggtggcac aactggcaac gaacagagtt
     1981 gcccgcaagg cgctcgtaga acatcgcctc gtgcactgca cacttccccg cgaacgtgct
     2041 aatcgaaaaa catgtcgtcc gtgcgaaacg gaggcgcccc cataatgaga gcgatcacgt
     2101 tcccgcgtgc ggtgtgccgc acgccgggac gaatcagtac cgcggttccc tgcgagaggg
     2161 cgatcatctc cccgtcaagc tccagctccc cttcaccttc cagcacgtag tatgtttccc
     2221 acgtggcacg gtggtagtgc ttcttcgaat cggagattcg aagaagggag accgagaaac
     2281 cggcgccatc gtcctccgtg aaaatcgatt tccggaagcc gcaggtggac ctcacctcgt
     2341 ccaaaccctc ggttgtccgt acgatgtact ttggcttttc gcgcccgtcc atttcctgat
     2401 cactccagtt gctcgcggct ttgccgacgt attgcctgtt ccatgtccat ctctatttcc
     2461 cgtcttcgaa gcgtttccct cttgtcgtgg atctgctttc cttttgcgag gcccagttcc
     2521 acttttaccc tgccgccttt gaaatacaac gacagcggca cgagtgtcaa gcctttttcc
     2581 tctgaacgac ccttcaagcg tccgatctca cgtcggtgga gaaggagctt gcgaggacgt
     2641 tcggggtcat ggttataccg gttcccgtga tcgtacgggc tgatatgcag cccgtaaaga
     2701 acgacctcgc cacgttccac gcgggcgtag ctgtccttca ggttcacatt gcccagccgg
     2761 agcgacttca cttccgtccc caccagagca attcccgcct cggttgtctc aagaatggag
     2821 taatcgtgaa acgctttgcg gtttttcgtt atgattttta tcccggccgg cattctgtca
     2881 ctccggtttt ttcgagaact tgacgccaaa agaacgtaac caaagtcttg ttccaaaaca
     2941 ttcggtcgca ctccctgttg ccgcaacaca tgacttgcgt accatttgtg caaaggccgg
     3001 ctctgatgtg attagggagg gaagatgcca cttcttctcg gtgctttgat gctgttgtgt
     3061 gccagttgtt cgcccgccac ttcggattgt ctggacacgc tgtcggaaat cggaagctcc
     3121 ccggagatcg tagccgcttc ccgtgcctcg gtgttcccgg acacttcttg cgggggcctc
     3181 ctcttttcga tcggaaccgc cgaatatgcc caggcttcat tcatcgccgc ccggaaggca
     3241 tttgaggccg cgaccgtttt ctggccggat tcctccaaag catggaacgg acttggactc
     3301 gcaaattacc ggatccgtga ttattcggca gccgagcgcg ccttcaaacg atcgctgcgc
     3361 cttgcgccga ggcagatcga tgtatacagc aacctcggcc tcgtattcct cgctcaggct
     3421 cgttacattg acgcaacggc ggccttcatc gccgcactgg gcattaaccc gggatacacc
     3481 gaggcaaggt tcaatctcgc gtacacctac gacctgcaag ggaggcctga ccttgccgaa
     3541 ggcgagtact accgcgtgct tcgcgaccac cccgaccatc caaccgcgcg atacggactg
     3601 gctgttcttt tccgaaaggc cgaacggttc ggcgaagcgc tggcccagct cgattccgcg
     3661 ctcgcaactc acccggatta cgcggacgcg ggagtggaac gggggttcct gtttctgcgg
     3721 cgaaacgagt attcccgtgc tgcgggggac ttccgtcgcg tgctcgattc ccacccggaa
     3781 aatgctcagg cgcggtacgg cctgggactg gcgtacgcgt ttcagcgcca gtgggtagcc
     3841 gctcacgcgc aatacgaaag cctcgcggtg gccgatacga cgtacgcccg gcgcctgcgg
     3901 cgtgttctgg ggaactgagc ccataaaaag gagaggagca caacgcgctc ctctcctttg
     3961 ctccagaaca atactcagtt cgcgatttac ccgatgctgt agaacgcgct tctcccggca
     4021 aacttggcaa cgcttcccag cgcttcttcg atccggatga gttggttgta cttcgctacg
     4081 cgatcagttc tgcagggcgc gccggttttg atctgaccgg cattggtagc caccaccaga
     4141 tccgcaatcg tggtatcctc ggtctcgccc gatcgatggc tcaccaccgc tgtgtacgat
     4201 gcacatttgg ccatctcgat cgctgcgagc gtctcggtaa gcgacccgat ctgattgagt
     4261 tttatcagaa ttgaattcgc gacctcttcg tcgatcccct tctgcagcct ctcagtgttc
     4321 gtgacgaaca catcgtcgcc gacaatctgg atcttgtcgg tcaaacgctc cgtgagaagt
     4381 gcccaaccct cccagtcgtc ctcagccatc ccgtcctcta ttgagatgat ggggtatttg
     4441 gcaacccagc gctcgtaaaa gtccaccatt tccggcgcgg aaagcgtcct gccctctttg
     4501 gcgagaacgt atttgccgtc ttcgaagatc tcgctggcag cggggtcaag ggcaatcatg
     4561 atgtcatcgc cgggcttgta acccgctttt tcgattgcct gtagaatcac ttcgatggct
     4621 tcttcgttcg acttcagcga aggggcaaat ccgccctcat cgcctatgga cacgttgtac
     4681 ttcttcgcgc tgagcacctt tttgagagcc tggtacgtct cagcgcacca ccggagcgcc
     4741 tcactgaacg taggagcgcc caccggcatc accataaact cctgaaggtc cgtcgagtcg
     4801 acggcgtgtt tcccgccgtt caggatgttc atcatgggca ccgggagaac tcgagcggac
     4861 ggtccaccga gatactggta gagtggcagg ccgcaggttt ccgccgctgc ctttgccacg
     4921 gcgagagaaa cgccaagaat ggcgttcgct cccagattgc tcttatccgg agtaccatcg
     4981 agtttgatca tgagttcgtc gatttccacc tgatcggtgg cgtcaagtcc ttcgatttca
     5041 ggcgcaatcg tctcgttgac atttttgacc gccttcaaaa cgcccttgcc gccgtacgca
     5101 gcctcgccgt cgcgcagttc caaagcctca aatgctcccg tggaagcgcc cgatggcact
     5161 gccgcacggc cgaacgaacc gtcttcaagt accacttcaa cttcgacggt cgggttgccg
     5221 cgcgagtcca gaatctggcg ggcatgaaca ctctcgatca tgctcatggc gtatcacctt
     5281 tctcgtgtat cgatgggaac gaacgctgtt cacaaacgca aggttgcctc tgtgaaccag
     5341 ctgctgattg tgctgcaaag caaataacgt gagtccgtcg gatcctggag cacgtcttta
     5401 ttcgtcaaag acgatctctc acccggcagg cggtgccaca agtttcaacc attcaagaac
     5461 gatggacaca aatcgtgggc tgatagcctt tttgtcttga ccgggatccg gaatctcgtc
     5521 gcgcagatcg tgtcggagcc ccacagccgt gtggacctga gaaagctgac gtcgccctat
     5581 cgctcgtcga aaccgttcaa tgtccagcaa gggcaacaaa ctgtccgcag tgccctgcac
     5641 ggccagcgtc ggaacactga gcctcgccac gcggtcggtc atttcgcccg agagcacaac
     5701 ccaggatcgc cagcattcca cacggccgtt cattacgaaa tccgagtcgt tgtacttatc
     5761 ggtccagata tcatcgaaaa actgctccaa ccggcggagg ttcaccgaat cgcggaacgt
     5821 tccggagaga agcgtcctgt ccaccgaacg agccggtgtg ttcaccagtc cgagcgcgtg
     5881 cacacggccc gcggaacgct cggcggccag tgccgcgata accccgccct gaccgaaccc
     5941 gaggaacacc accctggccg tatcgattcc ccggatattt cgcgcgctta gaaacccgag
     6001 cagtacttgc gaggtcagtt tgtcagggca ccgaactacg agactatcgg aaggccacgc
     6061 gtcgcgcaga ggcctccaca cggcgtatcc attggaaaca agtgcggcct gcagttgttg
     6121 aatctgattg gcttgtgtcc tgcggggatc gatatctatt ggcggtccgt catcaacgag
     6181 aactacaagc ggcaccggcg caaacgtcct ccgcgccggc aactggacct cgcccacaat
     6241 ctcacttcct gtccggggca gaaccagccg aaccacttcc gccggaaaac ggaaagacga
     6301 gccgcacgag catacaaaac cggccagaac gacacaacac agccagttcg aggccacgct
     6361 cgttttcata cgttccgacg cctcggtgtg catacctggg attctccccg ttcacacagt
     6421 ctgcgaccgc actacaatca tctgatcccg ttccgggcca attcccacat aacggaccgg
     6481 aacacccgcg atctcttcaa tccgccggat gtaacgcctg gcgttttccg ggagttcctc
     6541 gaaacaccgt gcggaagtag tgtcggtttc ccagccgggc aacgtgtcat acaccgcctt
     6601 cacacggtcg aagtcctccg tgcgcgcagg aaccgtgtgc agaggtgttc cgtcaagaac
     6661 atactcccgg cagatgcgta actccgaaag agcgtccagg cggtccagcg ccgtcactgc
     6721 gaggccggtc aacccgttga tacgcgctga atactcaagg atgactccgt ccagccagcc
     6781 acaacgacgt gggcgccgcg ttgttgctcc gtactcattc cagagctcgc gcatccggtt
     6841 tcccatctcg cattcgaact cggtcgggaa gggaccgtta cccacgcgac tcgtatacgc
     6901 ctttacaatg cctatgactt ccgaaatcgc ggtcgggcca atccccgtgc ccgtgcacgc
     6961 accacccgcc gatgtgttcg agctcgtcac aaaggggtag gtaccgtgat caatgtcgag
     7021 aagggtaccc tgggcccctt cacataggat cgctttgcct gcgttctggg ctgcgtgcag
     7081 gatcgtcgcg gtatccgcga tgaacggcct catctgctcc gcgtatccga ggtattcatc
     7141 agccactgcg tcggcattca ccggctctgc gccatacacc ctggtgaaca cctcgttctt
     7201 gtccgccgca attttccgca gacgcgcgcg gaaccccatc gcatcaagga ggtcccccgc
     7261 gcgaagtccg tgttcacggg aaattttgtc ataataggtc ggaccgattc cgcgcttcgt
     7321 ggtcccgatg gcgccggtcc ctcgagccgc ctcctgaata ccctcgagtt gcttgtggta
     7381 cggcatcacg aggtgcgcgc ggtcgctgat ccacaggcgt ccctccaccg aaacgccccg
     7441 tccgcggaca tggtcaagct cctcagggaa ggccaacggg tccaacacaa cgccgttgcc
     7501 caccacgcac acctttccgg gatgcaggat tccggcagga atctggtgga agatccacgt
     7561 ctctccgtcc gcaatcaccg tatgccccgc attcgctccg ccttgaaaac ggacaacgat
     7621 gtcgttcttc tccgtgagga aatcaacgac ctttgctttc ccttcgtcgc cccagtgagt
     7681 tcccacggca acagttaccg acatggcgct atccttttct cagatcaccc tgaacgaggc
     7741 gttgccccgg agagatgcct tcaaacgcct gcaaaaccgc gcggcggagt cacctccacg
     7801 cagaaacgaa aaaacgaccc agccgccacg ccggcggttg agccgtacct gaatatatct
     7861 gaacggcttc ctctagtgca actatgcgta cgtgtacacg cgtgcacgca ttcgcatgtg
     7921 ccgggtggac gtctgaacgc cggtttcgct acattgagga ttccctcact catggaacac
     7981 ctcacagagg tctgcccgtg gaacagtggc cgacgcttgt catcttcgcg gcaacgtata
     8041 tcggcgttgc cgtcgggagg gtacccggcc tgcagatcga caggaccggg atagccttga
     8101 tgggcgccat cggcatggta ctcgtcgggc agatcaccgt caaagaagca ctcgacgcgg
     8161 tggattttcc cacgatcctc cttctttacg cgctgatggt agtctcagcg cagttcaggc
     8221 ttggtggctt ctacacctgg gtcgccggac gtgtcgcgag gctagcgcgg aggccggtgc
     8281 ttctgctcgc tgcgctgatt tccgtcacgg ccgttctctc cgcgctggtt gccaacgaca
     8341 tcgtctgcct tgcttttacg cccgtcctgt gcatcgccct tcaacgagcg gggctgaacc
     8401 cggtaccgta tttactggca ctggcttgcg cgagcaacat cggatctgca gcgacgatca
     8461 ttggaaaccc tcagaacatg ctgatcggtc aggtcggccg gctcgatttc gatgcgttcc
     8521 ttttctggtg cgccccgccg tcattagcgg gcttgctcgt ggtttttgga gtgtgtctcc
     8581 tgtcgttccc gcgtgagttt ctcgccgacg gtcaacacca tggcgccggc agcccgaatc
     8641 cgcctgaatc tctggaaggc ccccccgact tcggagggga gctggacgcg catcagactc
     8701 tcaaggggat catcgtgacg atcgtcctcg ttgcggcgtt tttcacgtcg attccgcgcg
     8761 aagtgagcgg cctcgccgcc gccgcgctcc tgctcgcgag ccggaagata cattctcgtg
     8821 ccatcctcgg tttcgtggac tggcatctca tcacgctgtt ctgcgcgctt ttcattgtcg
     8881 tccacggcat cgaggcgggt gggttccccg ctgccgccgt ggctgcgctg aaggagtacg
     8941 ggatcagcgt tggctcccta cccgtgcttg gcacagtggc cgttgttctc agcaatctgg
     9001 tcagcaatgt tcccgcagta atgttgctga tcgggcagct cccgcaagga aatccggtcc
     9061 cgtggtacgt gctcgcgctt acgagtacac tggccggaaa cctgatcctt gtggggagta
     9121 ttgccaatct catcgtcgta gaacaggcgg agcaacaggg agtccacgtc tcgtttgccg
     9181 tgcacgccag ggtcggcgta ccggtgacgt tgatcacgct ggcacttacg cggctttgga
     9241 tcgtgttggc gctatggatg acggggtgac gcgaggcgct tccccttggg cggggtcctc
     9301 acgccacgcc gcacttatct ggttttctcc ggactgggct tcgggatatc gagggggatc
     9361 gccaccgagt cggcgtctcc gccacggcgc acgtaggcac tcatgacaga actccactga
     9421 tggggtgact caatcttcgg ccccacctcc gcaataaggg caatttcgtc acattctgta
     9481 aacttcgggc agttgaggca atcgctccat accttgtggg gcagttcgtc tttgctcact
     9541 tcgcgaaatc ctgatttgcg ggcgagtctc gccatggcgc catccggaag aacaagcatg
     9601 aaaacctgat ggacgccaag tttccgcgca tcgtccagca ggctgttcaa gatggccttc
     9661 ccgtaacccc ggtgctgaaa cccttcccga accgcgacgg atcggatttc ggcaaggtcg
     9721 taccagagaa tgtgaagagc tccgcacccg acgactttgc cgtcctcttc caccaccacg
     9781 tagtctctga ggttttcata catccggttc agtggccgcg gcaacataag gtttcgttgt
     9841 gcgaaactgt ttatgaattg cgccatctct gcgacgtcgg atatccgcgc tgcacgaagc
     9901 atcttgccgt tcactccttc agactggccc gggcgagagc cagttgctgc accaccgcgt
     9961 tgcgacccgt gcccccgaaa aggttccttc tgtccgtgct tgcggccggt gagagcgtag
    10021 aaacgtcctc tcgcttgaac aacggacttg cctgagcgaa ctcctcccac gggagttcca
    10081 ccaggctgca cccgcgcgca atggcctttc ccaccaactc acccacaacg cgatgtgcat
    10141 ctcgaaacgg cagcccccgt ctcacaagca catcggcaag gtcggttgca tacattcccg
    10201 gttcgattgc cgcacgcatt ctttcgtggt tgaaaactgc tgtctccacc acgcctttca
    10261 ggactcggag gcagatatcg gccgtttcca gggcatcgaa aagcggctct ttgtcctcct
    10321 ggaggtcctt accatacgtg aacgcgagac ccttctggat ggtcacaagc gccatcagat
    10381 ccccgaatac ccgtcctgat ttcccccgaa cgagttcgag agaatccgga ttcttcttct
    10441 gcggcatcat gctgctgccg gttgcgtacg cgtcgtcgag ctcgacaaaa ccgaatgcgg
    10501 gactcgacca caccacccag tcctccgcca gtcggctgag gttggtcatc aggatggcga
    10561 tcgcggaaac cgtttccgtc agatgatcgc ggctgctgac tgcatccatg ctgtttcgcg
    10621 tggggcgcgc aaaaccgaga cggcgggcga caaactccct gtcaatcggg aaagccgtcc
    10681 ccgcaagtgc ccctgatccg agcggcatct cgtccgcccg ccggagtgca tccctcaacc
    10741 tccctttgtc ccggtccaac atccagaaaa acgccagcag ataatgcgcg agccgtaccg
    10801 gttgtgccgg ctggagatga gtgtaaccgg ggagaaaggc gtccacttcg gcttccgcgc
    10861 gtttcaggac gacggcctga agctgtgaca caagttcagt cagcgttcct accgcacgac
    10921 ggaggtacag gcgcacatcg agtgcgacct gatcgttccg gctcctgccc gtgtggattt
    10981 tgccccccac ggggccgacg atttctatca gccgccgttc gaccgccatg tggatgtctt
    11041 cggtatccgg tcccgggcgg tagctcccgc ctgccagctc ctgtcgcacc tgctgcaaac
    11101 ccagaactat ggcggaccat tcctcgcttg tcaggatgcc gcactgtcgc agcgcatccg
    11161 cctgagcaag attaccctcg atatccgctt cccagaggcg aatgtcaaat ccgatggaag
    11221 cgttgaactc ttccatgact tgatcggtcg cgcgcgaaaa ccgcccgtcc cagagcttcc
    11281 ctgtggtcat acgaagccgt tcaccctttt ctctgtttca acatggcgat cgttctctcg
    11341 ggaagagaaa acagcttcag gaaaccggtc gcgtcgcttt gttgataaga agacgcgcca
    11401 aacgtcgcaa gcgcttcgtc gtacagcgag ttctcactct gaactcccgc gacggtgcag
    11461 ctacccttgt acagcttcat ccgcaccgta ccgttcatgt cacgctgagt cgcatctacg
    11521 aacgcatcaa tcgcctcgcg cagcggggta aaccatttcc cgtaatagac gagttccgcg
    11581 taccgttggg cgatgatatc cttgtaatgc agggtttccc ggtcgagcac gaggctctcc
    11641 aactccctgt gcgcggcgta gatgatcgtt cctccgggtg tttcgtacac cccgcgtgat
    11701 ttcattccca cgagccggtt ttccacgagg tcacagcgac ccacgccgtg tgccgcacca
    11761 agcgcgttca actcggtgag caggctcacc gagtctttcc gcacaccgtt gattcccacg
    11821 gggatgccgg cttcaaactg gatttccacg taacgaggaa catcaggagc gtcctccgga
    11881 tgaaccgtga gtacccagac gtctcgtgga ggttcctgcc acggatcttc cagtgctccg
    11941 ccctcgtggg aaagatgcca gatattccgg tcgcggctgt agatgctctt tcgggaagca
    12001 gtgaccggaa cattgtgggc cgccgcgtaa tcgagagcgt cctcacgaga ccggatcttc
    12061 caggcaggat cgcgccacgc cgcgatcact ttcagatcgg gagcaagcgc tgcatacgta
    12121 acctcaaagc ggacctgatc gttaccctta cccgttgcgc cgtgcgccag agcgtccgcc
    12181 ccggtttccc gcgccacctg cacctgcgcc tttgcgataa tcgggcgagc aaacgacgtt
    12241 ccgagcagat acttgcgctc gtaaatcgcg ccagctttca gtgtcggcca gatgtacccc
    12301 tcgatgaact gctgcttcag gtccagaaca acgcaatcac tggctccgct tcgaatcgcc
    12361 ttttcctcga tgccatgaag ttcgtcttct ccctgaccga ggtccgccac tacggcaaca
    12421 atctcacacc cgtacgtctc tttcagccag ggtattatta ccgacgtgtc gagaccaccg
    12481 gagtacgcca gagccacctt ttttacttca gacgccatgt cactttcctt tcattcacca
    12541 gcagtctttc gtgtatcacc gtgcctcgag gatttccgca aacacatcgg cgacgtggtt
    12601 cagttcctct tctccgataa caaggggcgg gaggaacctc aacacgttgg gcccggcaac
    12661 acacacgagg atatgccgtt ttctgaactc tgccacaatc ggcgctaccg gttcgtcgaa
    12721 ctgaacaccc catatcatgc ccatcccgcg tatctcccgg atagcggaat attgttcctt
    12781 gagcgcccgc aaccttcctc caaacaacgt ggcattgttc cttacctttt ccaggaactg
    12841 cgggtccgca attttccgga aaacaacgag agccaccgca cagatgagcg gcccaccacc
    12901 gaacgtggca gcgtggtccc cgggctggat tgcatccgcc acatgttgct tgagcaaagc
    12961 ggcgccaatc ggcaagcctc cggcaagtgg cttggcaagg gttaacattt ccggttcaac
    13021 tccgtaatgc tcatggcaga aaagccttcc cgttcggcca agcccaacct gaacttcatc
    13081 aaagacaagc agggcgttgt gcctgtcgca ggccgagcgc actgccttca ggtattgctg
    13141 agtcgccggg tgcactccgc cttctgcctg cacgggttca agaaaaactg ccgccgtgct
    13201 gtcgtcaatc tgttcaactg tgtggtcgat atcgttgaac cgggcaaatc ggatgcccgg
    13261 cacgagcggt tcgaacgggg cctggtattt ctcctgcccc gtaaggctga tggaccccat
    13321 ggttcttcca tggaaggaac tgaacgccgc aaccaacccc gtcttgttct ccccataatg
    13381 cgtccgggcc caccgacgcg caaacttgat acacgcttcg ttcgcttctg ttcccgaatt
    13441 gcagaaaaag accctgtcag cggaggaatg ccgaaccagc tcttctgcga gcgatgccgc
    13501 gggaatcgtg taatagagat tcgagacatg gatgagcttc cggctctgct cgcgcaatgc
    13561 ctccaggaca tcagcgtctc cgtaaccaag cgcgtttacg gccaggccgg agacgcagtc
    13621 gagatagcgt tccccttccg cgtcgaagag gtagacccct tcaccgcgtt cgaaaacgac
    13681 gtcagcccgc gcgtagttct gcaggaggaa ttccttgctt cttcggatca cttcccggga
    13741 aacgcctttc cccgccggga gggacgccga ctgcgaatca tcatgcactt caccaacctc
    13801 ctgagcatcc gcatctgccg tggcgcgaca ggtgccagaa tcaatccgtt atcactgttc
    13861 catgagatcg aacacccgcg aggtgttcag agatggttgc cgccccgtcc caggcaagaa
    13921 tgtggacccg gccgacgcca cgccgaacgg cttccacaca cgatcgcact ttaggtatca
    13981 tgccaccggt tatctgtccg atcgcaatca acttctccgc gtcagctcca gtgaggagcg
    14041 gaaccgtacg cccttccacc agcactccgg gcacatcggt aacgaacacc agttcgtcca
    14101 ccttcatcgc catcgcgatg tcggccgcgg catggtccgc gttcacgttg tacgccaacc
    14161 cgtctccacc tacgctgatg ggggacacca caggcagaag cccggcattc agaagggcaa
    14221 caagcaccga agggtcgacc ttctcgattg cgccgacgta cccgagcctc ccatcgccgt
    14281 agggccgcgc ttgcaggaga gcaccgtcta cgccgctgat cccgactgcg cgtgccccgg
    14341 ctgacaacag cgcacgcacc aatcgcttgt tgacttgcgc cgacagcact gcttccaccg
    14401 tctctactgc cgcggcgtca gtcacccgca ggccgtcgac aaacgtcgtc ggttgtccga
    14461 gccggtccag cgcgcgattt atgtccttcc cccccccatg aacgaggatc gtccgtcccg
    14521 cgccgagacg cgcgaggtca aacgccagtt ctccgatcag ccccggggag tcgagtgtcg
    14581 aaccacctat tttgacgagc gccaacatcg ttttcctcgc gctgcgcgtt tcttacaccg
    14641 gaaggccgca ttcttcctcg aacccgcagg caatgttcat gcactggagt gcctgaccgt
    14701 acgcgccttt taccaggtta tcaatcgcac acaggacgat cgcccgtccg gtgtccggca
    14761 ctacatgcac tgatacatcc gcgtagttcg tgttctgtac gaacgccgtt tcgggcagat
    14821 gaggcagcac gcgaacgaat cgttcgccgg agtattcact ccggagaaga tccagaagct
    14881 ccgatgtgcc aagagggtgt gtcaggtcca cgtacatcgt ggaaagaatg ccctggttca
    14941 tgggcgtcag gtgcggtgag aacaccacgc gcaccgcctc gcccgcgaat ttgctcagtt
    15001 cttgttctat ttcaccaacg tgccggtggg atcggcccac attgtacggc gcgatgttct
    15061 cgttcgtttc aacgtaatgt gtccgctggc tgagtttcct tccggcaccg gaaacacctg
    15121 atttcgcgtc cacgataatc gtgcggggat cgataaggcg atgcttcagc agcggcgcaa
    15181 gcccgatctg caccgacgtg ggatagcacc ccggaacagc aataagcctg gcctcacgaa
    15241 tccgctcacg atgaaactcc ggcaacccgt acaccttcac gcccagtagc tccggggcag
    15301 tatgcgaaac cttgtaccac gcggcatacg aatcgggagt atccatcctg aaatcggcgc
    15361 ttaaatcgaa gaccttgcac cctttgtcca gaagtcgcgg cacaacagcc atcgccgctt
    15421 catggggaag gcagaggaac gcgacctcgt tcgcctgctc cactgcttcc tccaccgaca
    15481 ccatcacccg gtcggtcagc cccgccatgc ggggaaagac tgctgaaagt cgttcgcccg
    15541 cgtgtcgatc cgctgaaaga gccgccacat taacgcccgg atgccgcagg caaaggccgt
    15601 aaagccctgc gccagtatac ccggacgccc cgaaaatcgt cacagaaacc tgtctcttca
    15661 tccttccagt acctgctgaa gctggcgaag cacatggttg gcatccgccg gtgtacgaca
    15721 cacgacgaga acggtgtcct ctcccgcaat tgtgccgaga atgtcgctcc atttctcagt
    15781 atccagcgcc cacgcaactc ctgccgcgtg gccggccacc gttttcatca cgataagttg
    15841 ccctgcggcc tcccacgaaa agaggaactc gcgcagcgca aactgcagac gcccggcggg
    15901 atgctctacc cccccctccg tcgggagctg gtagcggtat ccggaatcgg ggagaggcac
    15961 tttgatcaaa cccaattcag cgatatcttt ggacaaagtc ggttggctga cggggaatgc
    16021 acgctctctc agtgcattcg ccagttcagc ctgtgttctg atcgtctgcg cccggacaag
    16081 gcgaacaatc tcccgctgac gggcaagctt ctggctggtt ttcatccccg cgccctgttg
    16141 gtgatattta tcggtccttt gatattttta tttatctcgc caattaatga tacgagtcca
    16201 cctcacatga tgtcaatgcg tcaccgggca taatttctcg gaactaaccg atgcggaaac
    16261 tcatcatcgg aattattcgc cttgctcgac cggtccagtg ggtgaagaat gtcttcgtcg
    16321 ccgctgctcc ggtctatgca gggcaaatat tcgtgcccga gatcttcggc aagacgtccg
    16381 ccgcggtcct gttgttctgc gcgctgtcgt ccggcgtcta catcatcaac gattacttcg
    16441 atatggcacg ggatcgcgaa catcccgtca agaagaacag gcctctcccc tcgggacagg
    16501 ttccagtgcc tatcgcgttg gcggtcggat tcgcattgtt ggccggcagt ctcttcgggt
    16561 gcgccttcgt ttctccgggc ttgcttgcgg ttgcagcggc atacgtctcc gtgaacggtc
    16621 tgtacacgct ctggtggaag caagtcgtcg tgctcgacat catgtgcgtt gcgtcggggt
    16681 tcgtgttccg agtggcagcg ggaggccttg ctgtcggggt ccccgtaaga ccgtggattc
    16741 tgctctgcac gctctgcctc gcattgctca tcagcctcgg aaagcgccgg ggagaagcgc
    16801 tgcttctcgg agagaacgcg gcgaaacacc ggcgc
//
LOCUS       DAOSOO010000051        16700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3478_length_16700_cov_5.152779, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000051 DAOSOO010000000
VERSION     DAOSOO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_16700_cov_5.152779"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1008)
                     /gene="nth"
                     /locus_tag="PLT86_05475"
     CDS             complement(340..1008)
                     /gene="nth"
                     /locus_tag="PLT86_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT36094.1"
                     /translation="MRGTMATGKRSARAEKERVAEIHRRINAEYPRSTTALEYRTPVQ
                     LLVSTILSAQCTDVRVNEVTKSLFQNYRTARDFAEAPIEELEEAIRPTGFFRNKAKNI
                     KNCCKTIVERFGGEVPRNMDDLVQLDGVGRKTANCVLGNAFGMNEGVVVDTHVMRLSK
                     RLGLSTSEDPVKIEQDLMRLFPRESWTLLAHQLIDHGRAVCNARKPKCGECLLADLCP
                     SAKP"
     gene            complement(1047..1397)
                     /locus_tag="PLT86_05480"
     CDS             complement(1047..1397)
                     /locus_tag="PLT86_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT36095.1"
                     /translation="MRYLILRLLLTCLSIYIAARAVDGVALVEPWAWWHLLLVGAVFG
                     LLNAVVKPILTFFSLPVLILTMGLFYFVINALILWMTPVFVPMLVVDGFVAALKGSVV
                     ISLMNWLFSWLVGI"
     gene            complement(1560..3005)
                     /locus_tag="PLT86_05485"
     CDS             complement(1560..3005)
                     /locus_tag="PLT86_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36096.1"
                     /translation="MTARVPQTSRIAFRVGASEQDITPPYGAYLSGSACGDRRPAQSV
                     LDRTYARAIVFDDGRARVCVISLDVTIITGEYSTRVRNDVSDATGIPIEAIMVFAEQT
                     HSAPSVGRFMLDPDFPLETTPETEYLWGSEAPYCEFASKKAVQAAIEAHDRLQPARVG
                     LARALNAEFSFNRRGVTRDGRIIMPKPAGRENQPFGITELCYMEGPMDPEVGVACFQG
                     LDMRPIAFLLHHTCHPVNVFGSRGTYRAVSSDWPGAWAEGMKRAYGSHVVPLVLNGCC
                     GNINPWNPFEPDARPDHRRMGKALAEVSEKLVHTMQFAEGAQVASCADCVSLPFREIP
                     GERRRMVAEILERSPEPPRSANGEVDPLWFTAASTRSVEILKQRETHMRYEMQVFRVG
                     DLAIVGLPGEPFVEGQLAIKTSSPAPFTFPTHMVSHYVGYLPTREAYARGGHEANNEV
                     TYWAKLAPGCLEAVAERAVVNIKTLFPDRVA"
     gene            complement(3025..3438)
                     /gene="nikR"
                     /locus_tag="PLT86_05490"
     CDS             complement(3025..3438)
                     /gene="nikR"
                     /locus_tag="PLT86_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOT36097.1"
                     /translation="MGGLARFSVAVGEDLLKRFDRLIRESGYSNRSEALRDLIRDRLV
                     AEQWAAGEEVVATITLVYDHHVRDLAERLTEVQHQRTDRIISSLHVHLNHDNCLEVIV
                     TRGSAHDVKELAGLLIGSRGVKHGGLVMTTTGEEL"
     gene            complement(3452..4549)
                     /locus_tag="PLT86_05495"
     CDS             complement(3452..4549)
                     /locus_tag="PLT86_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT36098.1"
                     /translation="MTRADDIALKRVRVRDFLRSHDLGGVLFTQQKSFSWYTSGGQNH
                     VGLTTEAGAAHLLATPDQDYLVANNIEAPRIMDEEGLSELGIQPLVYQWDSPETDATR
                     LVERVLAGQRYATDTGQFAGELNHLRYSLTPVEVERYRALGKDCAEVMSDLCLAVEPG
                     QTEWEVAAALSKRLWEREIVPAVVLVAADDRISKYRHPIPTANKIAKYVMVVMCGRRH
                     GLILSTTRLVHFGKLPAELKRKHNAVMRVDAAYIGATLIGADVDEVFQKGLDVYEETG
                     FKDEWMLHHQGGGTGYASRDFKGSLHCKERVQAWQAFAWNPSITGTKLEDTMIATPDG
                     PEILSMIRDWPSVEVPAGLWGSLRRADILVR"
     gene            complement(4785..5879)
                     /locus_tag="PLT86_05500"
     CDS             complement(4785..5879)
                     /locus_tag="PLT86_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36099.1"
                     /translation="MHFVECDTVSGAEIYQLTDGPRPADNIYGEQPYSSPCGTRIAVR
                     YYPHEGTDGGLSILDLVDGSLHPVLKKMPRFPAFHAWGEYLYFQEYVGEEMLLRRCHY
                     VNTTTEDVLVLPATGSQLSYGTVSPDVEYYAVNSHPDSTSSRLLLFGLVDGVHSVLAE
                     SSAQWFKHEQFSRDGRDAILIQANSADVSVVNLGVVYPGGGEIEWLPVDSRPLVPVGK
                     WPGRDRYTPRCTGHEAWIGKTDRVFLSTAYDSSSMTNVWSVSLSDEQPAAACVGEHVF
                     GHVATSGCGRFWIADAPSEEGIPIYIGSFATGSNSRLCISRTVHDGQQWSHTHPYLTA
                     DNRWVIFTSNRSGIPQIYGARLADGFLSSL"
     gene            complement(5932..6558)
                     /locus_tag="PLT86_05505"
     CDS             complement(5932..6558)
                     /locus_tag="PLT86_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT36100.1"
                     /translation="MSVQPLRTAEITADPMCAAMELRLPSGEHVVFRPLRAEDATLLG
                     EYFTSLTRETTRFYGPHAFDQATADQLCGSINYDDTIRFVALTRVDGREQIVAYFILV
                     LGTSESDGKRYADVGIRLDPAQDCTFAPSVREQFQNQGLGTPLFRHVIHVARRLGRRK
                     MVLMGGVQVLNTRGIHFYEKQGFQRVGGFEGPPGIFSQDMELDLLLDS"
     gene            6822..7658
                     /locus_tag="PLT86_05510"
     CDS             6822..7658
                     /locus_tag="PLT86_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT36101.1"
                     /translation="MASTIRVAMIQRAFVPFEREANIAIAERAIRAAARDGAHLVALP
                     EMFLTGYPHAPAKDSADWPQFVERLYGTAETADGTSARKLGEIARDAGVVLIAGICEI
                     RDGRLFNSAFVWDSKGERIGTYCKVHTCRFTALEDLCEDGDDWHVWPLAVADQLVRVG
                     VMICYDREHPESARVLMLKGADLIIVPNACGIDERRLIQLRTRAYENVVYIGMANHSK
                     PHNGQSVITNYWGDILTQAGEGDETIFADLDLEALRDERTHTIWGNNFRRPWKYGLIA
                     KT"
     gene            7722..9032
                     /locus_tag="PLT86_05515"
     CDS             7722..9032
                     /locus_tag="PLT86_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT36102.1"
                     /translation="MKSFVSRAHGVASNSLRRWNAQSVDFRWFILALCVSTVGGAMVD
                     SAFNNYLRDTFHLAADKRGQLELPRELPGFLVTVTSGLLFFLPEVRTAAVATAATAIG
                     IGGLAWAGANYGVMIGFMVLWSTGLHLMMPMRSSITLAFAKEGRQATLLGTTGSITTV
                     AAILGSAIGWWIFGTLGTNPRTYNAIFILGASLTGLSALILLKMRVPATAQDQPRPKL
                     VVKRRFIVYYLMSMINGARKQVFITFAPWVLITIFHRGPSTFAALAITSALLGIVVQP
                     TLGGLIDRLGERRILMMEGFFLFFVCLGYGFAHRISTGGTVILIVYACFVLDNLLFSV
                     GMARVTYVNKLAEKAEDVTATLSLGVTLDHVISMSIPILGGATWVIWGFEYVFAGAAG
                     LAVLSVFVASFVRVPRVPLRTPIVVTEEEGPGTCRTEEIGRTSA"
     gene            8996..9718
                     /locus_tag="PLT86_05520"
     CDS             8996..9718
                     /locus_tag="PLT86_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HOT36103.1"
                     /translation="MSNRRDWPHVRLMMATTLDGKIATSRREAARFGSPEDARRLETQ
                     VAWADVLLFGAATLRAYGTTYRVRREALLRLRQRRRKPPQPVTAVVTSSLDLPLEMPF
                     FTRQDVPRVIITAESEKDRATVQFCGLAEVIARGASAVDWNAVLAEFKTRGLKRVLLL
                     GGGHLNAELLSLGCVDEVCVTVSPFVFGGTDAPTIADGNLAAKHWRGEPQLRTSKLVG
                     EEVFCTYRFRKRSPINSSVRTQ"
     gene            complement(9803..12310)
                     /locus_tag="PLT86_05525"
     CDS             complement(9803..12310)
                     /locus_tag="PLT86_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36104.1"
                     /translation="MQIAVFVGFLLVSGASIRVEAQTWSTTHPGYPCTDARALGMGTA
                     IVSSTDGPAALAWNPAGLVEQVFPSLAGDLGSTSLRNSALFFGSPVSQDGAIGLGWVR
                     NRRILPYPGTPEGLDRFAAGVGIRLTDKLAVGMSGSFFTPQYRSAPDSAREAAGFSTD
                     IGALFSLTGRIRVGASIYDLTDAKVWREDGRVETLNPRSVRWGVSFDASDQVTFAYQR
                     DQGDRIGVEWFPVESMALRGGVGYRDRMWSRSGGVGFRSGSVRLDYAYAQLEQSYHQH
                     RVSVAMVFPRGRSAVQITKPQVRSLYASLGKAYTREPVASIELVNSSSTPVTARVELL
                     LPRFMTAPSSEEVTLRPYSRQRIFLRAVLSGEQIAAVADQPVQGVISLVNPATGEVWR
                     RHTVDTFLYRRGALTWDDVRKASAFVTPADPDVRAFVRESLRPYRSRLQQLPLTQEII
                     TTAMVLFEAAGEVGITYQSDPNTPFSEVRKTENPLDAMRRIGANTLTLAVKPVEIAAN
                     PIRNVISGPDSARGDTLGVIGNAVSGVSGMITGVGEIGEESLRLLLFPYQALAGDLNQ
                     NPVVDDILYPAELLRVRAGDCDDTTVLLCSLLECAGLTTAFVEAPDHIFMMVDTRLPV
                     DESPYVLPAPVERFVAFEGTWWLPLETTLFPQGFMAAWSNAAGVARSLASQGNLRVTR
                     VAEGWALFEPIEFHASPDEEIRFPDSRNLERRVTLQDSALAVLWKDSPVARDLLASVG
                     ASTGGSEEDKRRADAAFWLLFAGRLSDADQHLRVLEEHAYDPPRVLGLRAVYWFFSGD
                     VIRAEEFADQAYQAAPEIPDLLDLRTQIRSARSGVSE"
     gene            complement(12323..13063)
                     /locus_tag="PLT86_05530"
     CDS             complement(12323..13063)
                     /locus_tag="PLT86_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT36105.1"
                     /translation="MEQGFLNIAHRGFSGQYPENTIIAFRAALELGCTWLECDVRRTR
                     DGCFVVIHDQTVDRTTNGTGRVDSLTWNEVMRLDAGSWKDPKFSGERVPLLDELLTLV
                     SGQAQLVIELKVGKDSVPGVVSVVSRHCAFGWTVASAFEWEVLETVRREAPRWRTTWL
                     TTFEDLGPDEAINRCVDAGVDTIAPRASLIDVEFVRKAHAAGLLVRAWGVADDTGPGL
                     RRLIAAGVDGATTNHPNVLKEILNEPLS"
     gene            complement(13077..13349)
                     /locus_tag="PLT86_05535"
     CDS             complement(13077..13349)
                     /locus_tag="PLT86_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT36106.1"
                     /translation="MLPNVGPWEMIVILLVAFLVFGSKRLPEVARGIGKGIREFRREI
                     SGVTDEINGTRDAVRNAPPPPPVSASKEESEKKDEDVRNPSDPVIL"
     gene            complement(13376..14149)
                     /locus_tag="PLT86_05540"
     CDS             complement(13376..14149)
                     /locus_tag="PLT86_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT36107.1"
                     /translation="MKIGLKRVRGLLAKENRKSLGLFLVEGVRLVEEVLASPLTVECL
                     FHVEADPDSRLFRVIEAAASDGIPTQSITQKELDAICDTRTPQGVVAVARTPTWTEED
                     VWKSGTGDVVVVDNVRDPGNLGTLLRSAEAAGARGVLLMPGTVELTNPKVVRGSMGAF
                     FRLPVSYTHSIDDVLRGARSAGIPLVATSSHGGEPASLLLKWDAVALVLGGEAEGVAR
                     DWTVVCDCIVSLAQKRGTESLNVAVAGSIFLFRHLWRAV"
     gene            complement(14153..15193)
                     /locus_tag="PLT86_05545"
     CDS             complement(14153..15193)
                     /locus_tag="PLT86_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36108.1"
                     /translation="MKRMCSNAVGLRRQLIAVCVFFSALPESVRAVQVPANFVLAQEA
                     ALRSEFARAESLAVVMKREQPADPTADYAMAAIEQMRLFGCEGTMSPAELKRRVQIAV
                     RAGREQVARRPEAIWPRYVFGLSLGLSAVIAADEGSYWHAYRAGTESVEQLEEVLRRE
                     PGFSDALLALGAYHYWRGAAMRNWTWLPFIADTRSQGIREMKRAWREGATTQNTAPGM
                     IVWALLKDANYHEALAISSEIAARYPANRAFVTARADALFSLRRWEEAAAAYTQAVGL
                     YSADQFQCPGSVEARARRGIALAEMGKCSDALPDLRAAVLSPAHVGYGRDFKATQNRA
                     RVLLERCLEKGP"
     gene            15353..15973
                     /locus_tag="PLT86_05550"
     CDS             15353..15973
                     /locus_tag="PLT86_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36109.1"
                     /translation="MRKPLTKRQLAIYEYIAAQIRQRGIPPTIREICDQFGMRSSNGA
                     REALAALARKGYINRRERLSRGIELANPFDSLECSGTPIRVPLLRNPVKGARCIDEAT
                     GEKVLFIDSSFLPGTGRAFALLVPDGSLAKYGIRKGDLAIATECYEGQPGEILITAID
                     SRLVLVRVPATSDQEPSQQVPESHGGSPVFGKVNCVVRRFTGVETG"
     gene            16237..>16700
                     /locus_tag="PLT86_05555"
     CDS             16237..>16700
                     /locus_tag="PLT86_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT36110.1"
                     /translation="MKINKVVIPVAGLGTRLLPATKSQPKEMLPVGRKPVVQYVVEEM
                     VEQGLRYLLLITGSNKHSIENHFDNDPMLIERLLSTEDDDLLREVDLRPEGAQIFYTR
                     QMIPPGAKKPAGLGDAVRHARAFVNDDPFVVALGDTIIHTDGKPDLISRMTQT"
BASE COUNT         3476 a         5045 c         4825 g         3354 t
ORIGIN      
        1 tgatacggca aagtctgtcg tttgcactcg gcgctttgtc cccagttagg gcagctccag
       61 aactgagatc cggggttttt gcccctgcga gcggttttca attccatctc tgaaccgcaa
      121 ttcgggcatt tgggcgcgtt cacgagcact tccttcctct gtaacctcac ttcggcgggc
      181 gtagccatcg catttccgac gcttttccaa aaagcgccgg catcaaaggt attacgacct
      241 gctgaccgaa tcaatgtgcc ttgtgaggag gggctcttcc gagccggcac ggccgagatc
      301 agcttggacg gccgaccctt gttggatgag gctgggaaat cagggcttgg ctgaggggca
      361 tagatcggcg agcaggcact cgccgcattt cggtttgcgg gcattgcaca cggcgcggcc
      421 gtggtcgatc aactggtggg cgagcagggt ccagctttcg cgaggaaaaa ggcgcatcaa
      481 atcctgctcg atcttcacgg gatcttcaga agtgctcaag cccaggcgtt tcgacagccg
      541 catgacatgg gtatccacca ccactccttc gttcatcccg aacgcattcc ccagcacgca
      601 gttggctgtt ttgcggccaa ctccgtccag ttgcaccaga tcgtccatgt tccggggaac
      661 ctcaccaccg aagcgttcca cgatggtctt gcagcaattc ttgatgtttt ttgccttgtt
      721 gcggaaaaag ccggtcggtc ggatcgcttc ctccagttcc tcgattggcg cttcagcgaa
      781 atcgcgtgcg gtacggtagt tctggaacag actctttgtt acttcgttca cgcggacgtc
      841 agtgcactgg gcggaaagta tcgttgagac gaggagttga accggggttc tgtactcgag
      901 ggcggtggtg gaacgagggt attccgcgtt gatgcggcgg tgaatctccg caacgcgttc
      961 cttctcggcg cgcgcgctcc ttttccctgt agccatagtt cctctcatcc tgcctccacc
     1021 tgattctggc gccggaacgg agtgctctag atgccaacca gccaactgaa aagccagttc
     1081 atgagcgaga tgaccacact tcctttcagc gccgcgacaa agccgtccac cacgagcata
     1141 gggacgaaaa ccggggtcat ccaaaggatg agcgcgttga tcacgaagta gaaaagcccc
     1201 atggtgagaa tcagcacagg aagggaaaag aacgtgagga ttggcttcac gacggcgttc
     1261 aaaaggccga agacggcacc cacaagaagc aggtgccacc aggcccatgg ctccaccagg
     1321 gcgacgccgt ccaccgcacg ggcagcgatg tagatggaaa gacacgtaag caaaagcctg
     1381 agtatcagat atcgcacgga tacccccgat cggaaatacg gttggccctg ttctctctct
     1441 tcggtttgtc taccggtttc agagtgatta ccaaatcgtc gcctgtcaaa agttggtttt
     1501 tgcaaacgct gttacccttt ctcgcgccca tcgcggtccc cggccagtgt ggagaatagc
     1561 tacgccacac ggtcggggaa gagtgttttg atgttgacga cggcacgttc tgccactgct
     1621 tcaaggcagc caggggcaag tttcgcccag tacgtgactt cgttgttcgc ttcgtgtccc
     1681 ccacgagcgt aggcttcccg cgtgggcagg tacccgacgt agtgagacac catgtgggtg
     1741 ggaaacgtaa acggtgcagg cgaagaagtt ttgatcgcca actgaccctc gacaaaaggt
     1801 tcgcccggca atcccacgat agcaaggtcg cctacgcgga acacctgcat ctcgtaacgc
     1861 atatgcgttt cacgttgttt gagtatctcc acgctgcgtg tggacgcggc agtgaaccag
     1921 aggggatcca cttctccgtt tgcacttcgc ggcggctccg ggctcctctc gagtatctcg
     1981 gcaaccatgc ggcggcgttc gccgggaatt tcacggaatg gaaggctgac gcaatccgcg
     2041 caactcgcta cctgcgcacc ttcggcaaat tgcatcgtgt gtacgagctt ctcgcttact
     2101 tccgccaggg ccttgcccat gcgacggtgg tctggacgcg cgtccggttc aaacgggttc
     2161 catggattga tgttgccgca acagccattg agaaccagtg gaacgacgtg cgaaccgtac
     2221 gcacgcttca tcccttcggc ccatgctccc ggccaatcgg aggagaccgc ccggtacgtt
     2281 cctctggagc cgaacacgtt caccggatgg cacgtatggt gcaaaaggaa cgcgatcggg
     2341 cgcatatcca gaccctggaa acacgcaacg cccacttcgg gatccatcgg accttccatg
     2401 taacatagct ctgtgatccc gaagggttgg ttttcgcgcc cggcaggctt tggcattatg
     2461 atgcggccgt cgcgcgttac gccccggcga ttgaacgaga actccgcatt cagggcacgg
     2521 gccaggccga ctctcgcggg ctgaagccgg tcgtgtgctt cgatggccgc ttgaacagct
     2581 tttttcgaag cgaactcgca atatggagct tcggagcccc agaggtactc ggtttcgggg
     2641 gtggtttcca gcgggaaatc cgggtccagc atgaaacgcc cgacgctggg cgcggaatga
     2701 gtctgttcgg cgaacaccat gatcgcttca atgggtatcc ctgtggcgtc gctcacgtca
     2761 ttgcgaaccc gggtggagta ttcacccgtg atgatcgtaa cgtcgaggga gatcacgcag
     2821 actcgcgcac gcccgtcgtc gaacacaatt gcccgtgcgt acgtcctgtc cagcaccgac
     2881 tgagccggtc tgcgatcgcc gcacgcgctc cccgagagat atgctccgta cggcggggta
     2941 atgtcctgct ccgacgctcc cacacggaaa gcgatacgcg aggtctgcgg aacacgagcg
     3001 gtcatgccgt ttactccaac ggtttcacag ttcctcgcca gtagtcgtca tgacgaggcc
     3061 tccatgtttg acaccacggg acccaatgag gagcccggca agctccttca cgtcgtgggc
     3121 ggaaccccgg gtgacaatca cttcgaggca gttatcgtgg ttcaggtgaa cgtgaaggct
     3181 ggagatgatt cgatctgtgc gttggtgctg gacctctgtc aaacgttccg ccagatcacg
     3241 cacatggtgg tcgtacacaa gagttatcgt ggcgacgact tcctcgcctg cagcccactg
     3301 ctcggccaca aggcggtctc gaatcaggtc acgcagagcc tctgagcggt tcgagtaacc
     3361 ggactccctg attagcctgt cgaaacgctt gaggaggtcc tctcccaccg cgacgctgaa
     3421 acgcgcaagt cctcccatgc gtgctcctga ttcagcgaac gagtatgtcg gcgcggcgga
     3481 gcgaccccca taaccccgca ggtacctcga cggaaggcca atccctgatc atggacagga
     3541 tttccggccc atccggagtc gctatcatcg tgtcttccaa ttttgttcct gtgattgagg
     3601 gattccacgc gaacgcctgc caggcctgca cgcgctcctt gcagtggaga gagcccttga
     3661 aatcgcggct cgcgtagccc gttccgccgc cttgatggtg caacatccac tcgtctttga
     3721 accctgtttc ctcgtagacg tcgagccctt tctggaaaac ctcgtcgacg tccgcgccga
     3781 tcagggtagc gccgatatag gccgcgtcta cccgcatcac cgcgttgtgc ttcctcttca
     3841 gttccgccgg tagtttcccg aaatggacaa gtctcgttgt tgagaggatg agaccgtgtc
     3901 ttcgaccaca catgaccacc atcacatatt tcgcgatttt gttcgccgtg ggtatcggat
     3961 ggcggtattt cgagattcgg tcatctgccg ccacaagcac cactgcaggg actatctcac
     4021 gctcccacag ccgtttggaa agggcggccg ctacctccca ctctgtctgg cccggttcaa
     4081 cggcaaggca gagatcactc atcacctcag cgcagtcctt gccgagggcg cggtagcgct
     4141 ccacttccac cggtgtgagg ctgtaacgaa ggtggttcaa ttcgcccgcg aattgccccg
     4201 tgtccgtcgc atatcgctgg cccgcaagca ctcgctcgac gaggcgggtg gcatcggtct
     4261 caggactatc ccactgatag acaagtggtt gtatgccaag ctcgctcaac ccctcttcat
     4321 ccatgatcct gggcgcttct atgttgttcg caacgaggta atcctggtct ggagtcgcca
     4381 gcaggtgagc agcgccagct tcagtggtca atcccacatg gttttgccca ccgcttgtgt
     4441 accagctgaa agacttctgc tgcgtaaaca agaccccgcc aagatcatga ctgcggagga
     4501 agtccctcac gcgcactcgt ttcagcgcaa tgtcgtccgc tcgcgtcatt gtcgtgtcgt
     4561 ccttgtcgaa agggagcaac aatcaaaaaa ccctgtcaaa atatcgcaca agcgatgcac
     4621 taataccatg ctctcaactg ctcccctggt tcgacagcgg tataccgatg gaagtggcgc
     4681 tccacgcgct gtcacggcct ggttaagggg gattattcca ggtgagagtt cgaggaagcg
     4741 cggaatgacc gcgtattggc aagtggtatt ctgccagaca gctttcagag actggagaga
     4801 aacccgtctg ccaggcgggc gccgtaaatc tgggggatcc cggatcggtt ggaggtgaag
     4861 atcacccaac ggttgtcggc ggtgagatac gggtgcgtgt gagaccattg ttggccgtcg
     4921 tgaacggttc gactgataca gagcctgctg ttcgaacctg tggcaaagga accgatgtaa
     4981 atcgggatac cttcctcaga cggagcgtcc gcgatccaga accggccgca acctgatgtc
     5041 gcgacgtgcc cgaacacatg ctctcccaca catgcggctg cgggttgttc gtcagaaaga
     5101 gaaacgctcc acacgtttgt cattgaagac gaatcatacg ccgtcgaaag gaacacgcga
     5161 tctgtttttc caatccaggc ttcatgcccc gtacacctcg gcgtgtaacg atccctgccc
     5221 ggccatttcc ccactggaac caggggacgg gaatccaccg gcagccattc aatctcgccg
     5281 ccgccgggat acaccacgcc aagattcaca acggaaacat ctgcgctgtt tgcctgaatg
     5341 agaattgcgt cgcggccgtc ccgcgaaaac tgctcgtgct tgaaccattg cgcgctgctt
     5401 tcggcaagaa cggaatgcac cccatctacg aggccaaaaa ggaggagtcg cgaggacgtg
     5461 ctgtcaggat gtgagttgac cgcgtaatac tccacatcag gcgatacagt tccgtaactg
     5521 agttggctcc cagtcgcggg aagcaccaac acgtcttccg tagtcgtgtt gacgtaatga
     5581 caccgccgca gaagcatttc ctccccgacg tattcctgga aatacaggta ttcgccccac
     5641 gcgtggaacg ccgggaaacg gggcatcttt ttgaggacgg ggtgcaacga cccgtccacg
     5701 agatcaagga tggataaccc gccgtctgtt ccttcgtgcg ggtaataccg gaccgcgata
     5761 cgagttccgc agggagagga atagggctgt tcaccgtaga tgttgtccgc ggggcgggga
     5821 ccatccgtga gctggtagat ctccgcaccg gacacagtgt cacattccac gaagtgcatc
     5881 agcgagtacc ctcagacttc gtcgcgcgtc gggagaggta agcgcgcggg ctcaggaatc
     5941 cagaagaaga tccaattcca tgtcctgact gaagatgccc ggaggtcctt cgaacccgcc
     6001 tacccgctgg aaaccttgtt tttcatagaa atggatgccg cgcgtgttca ggacttgaac
     6061 tccacccatc aggaccatct tccgccggcc cagtctccgg gccacgtgaa tgacgtgcct
     6121 gaaaagcggg gtgcccagcc cctgattctg gaactgctcg cgaactgacg gggcaaatgt
     6181 gcaatcctgc gccgggtcca gccggattcc cacatcagca taccgtttgc cgtccgattc
     6241 acttgttccg aggacgagga tgaaatacgc gactatctgt tccctgccat caacgcgtgt
     6301 caaggcgacg aaacggatag tgtcgtcgta attgatggac ccgcagagct gatctgccgt
     6361 tgcctgatcg aatgcatgag gtccgtaaaa acgagtagtc tcgcgggtca gactggtgaa
     6421 atactcgcca agcaaggtcg catcttcggc gcgcagcggt cgaaacacaa cgtgttcccc
     6481 ggaaggcaga cgcagctcca tcgccgcaca cattgggtct gccgtgattt cggcggtacg
     6541 tagaggttgc acagacatgg gtttcgcgta tcacctttcg tgaaggattc tgaggacggc
     6601 ttgaagccgc agtgaatgga tcgtttccaa tgtagccagc gccgggcagg caacgcaatg
     6661 aggcaagagg ggagggggag agagctaaag aaccttcacg gcaggccact tggtggcccg
     6721 gcaacgcagg cggtactttt caagacaccc gtgtcccggc cgatctgcgc atgacctgcg
     6781 atggctgccc cgcaacgttc ccggaatgga gtggagaacc catggcgtca acgattcgcg
     6841 tggcgatgat tcagcgagca tttgttccgt ttgagcgcga agcaaacatt gcgatcgcgg
     6901 aacgtgcgat tcgtgcggcg gcgcgagacg gagcccacct ggttgcgttg cccgagatgt
     6961 ttctcacggg ataccctcac gcacctgcga aggattccgc ggattggccg caattcgttg
     7021 agcgcctcta cggcacggcg gaaacggcag atggaaccag cgcacggaaa ctgggagaaa
     7081 tcgcgagaga tgccggcgtc gttctcatag cgggcatctg cgaaatccgc gacggaaggc
     7141 tgttcaacag tgcgttcgtc tgggattcaa agggcgagcg catcgggact tactgcaagg
     7201 tgcatacgtg caggttcacc gcgttggaag acctgtgtga agacggggac gattggcacg
     7261 tctggccgct tgcggtcgcg gaccaactgg ttcgcgtggg ggtgatgatc tgttatgacc
     7321 gggaacaccc ggagagcgct cgtgttctca tgctgaaggg tgccgacctt atcatagttc
     7381 cgaacgcctg cgggattgac gaaagacggc ttattcagct ccgcacgaga gcgtacgaga
     7441 atgttgtgta cattgggatg gccaaccaca gcaagcccca caatggacag agtgtcatca
     7501 cgaattactg gggagacatc ctcacgcaag ccggcgaagg tgacgagacc atcttcgcgg
     7561 acctcgatct tgaagcgctc cgagacgagc ggacgcatac catatggggc aacaatttcc
     7621 gacgtccctg gaaatacggg ctgatcgcga agacttagct caaactgcgc cccggtgcgc
     7681 ggcaactgct ccgctcccac actccagact ggaaccacga tttgaagagc tttgtatccc
     7741 gtgcacatgg cgtggcatcc aacagcctcc gacgctggaa cgcgcagtcg gtggatttcc
     7801 gctggttcat tctggcgctt tgtgtgtcga cagtcggcgg agcgatggtt gactccgcct
     7861 tcaacaacta cctgcgcgac acgttccacc tcgccgccga caaacgcggc caacttgagt
     7921 taccccgcga acttccagga ttcctggtaa ccgtgacttc cgggttgctc ttcttccttc
     7981 ccgaagtcag aacggcggcg gttgccaccg cggccaccgc tattggaatc gggggactcg
     8041 cgtgggcggg tgcaaattac ggggtcatga tcggtttcat ggtgctttgg tccacaggcc
     8101 ttcacctgat gatgccgatg cggtcttcga tcacactggc gttcgcaaaa gaaggccgtc
     8161 aggcaactct gctcgggact acgggaagca tcaccaccgt cgcggcaatt ctggggtcgg
     8221 cgatcggctg gtggattttc ggcacactcg gcacgaaccc ccgcacatac aacgcgatat
     8281 tcatcctcgg agcgtcactg accgggctga gcgcactgat cctcctcaaa atgcgcgtgc
     8341 ctgcgaccgc gcaggaccag ccaaggccga agctggtagt gaagcgacgc ttcattgtgt
     8401 actacctcat gtcgatgatc aacggtgcac ggaagcaggt attcattacg ttcgctccgt
     8461 gggtgcttat cacgattttt caccggggac cctccacctt tgccgccctc gccatcacat
     8521 ctgcgctcct cggcatcgtt gttcagccca ccctgggagg attgattgat cggctgggag
     8581 aacggcgcat tttgatgatg gaggggttct tcctgttctt cgtctgcctc gggtacggat
     8641 ttgcgcaccg gatcagtaca gggggaacgg tcattttgat tgtttatgcg tgctttgtgc
     8701 tggacaacct ccttttctcc gtcggaatgg cgcgggtgac gtacgtgaac aaactggctg
     8761 agaaggcgga ggacgttacg gcaacattat ccctgggtgt tactcttgat cacgtgatat
     8821 ccatgtccat tccgattctc ggcggggcaa cctgggtaat atggggtttc gagtacgtgt
     8881 ttgccggcgc cgcaggtctg gcagttctca gcgtcttcgt cgcgtcgttt gtgcgcgtac
     8941 ccagagtccc cttgcgcacg ccgatagtgg ttactgagga agaaggcccg ggcacgtgtc
     9001 gaacagaaga gattggccgc acgtccgcct gatgatggcg acgacgttgg acgggaaaat
     9061 cgccacctca cgaagggagg cagcgcgatt cggcagcccc gaagacgccc gccggctcga
     9121 gacacaggtg gcgtgggccg atgtcctgct gttcggcgcg gcgacgttgc gagcctacgg
     9181 aactacctac cgggtgagac gggaggccct gcttcgcctc cgacagcgcc gcagaaagcc
     9241 cccgcaaccc gttactgcgg tagtcacatc ctccctcgac ttgccgctgg aaatgccgtt
     9301 tttcacccgg caggatgttc cccgagtaat catcaccgcc gaatccgaaa aggaccgcgc
     9361 aacggtgcag ttttgtggtc tcgcggaggt tatcgccaga ggggcgtccg cggttgactg
     9421 gaatgccgtt ctcgcggaat tcaaaacacg aggactgaag cgagtactgc ttcttggcgg
     9481 gggccatctg aacgcggagc tgctctccct cggctgcgtt gacgaagtgt gcgtaaccgt
     9541 ttcacctttt gtgttcggtg gcactgacgc tccgaccatt gcggacggga acctcgcggc
     9601 gaaacactgg cgaggcgagc cgcagctgag aacgtcgaaa ctggtggggg aagaagtttt
     9661 ctgcacgtac cgttttcgga aaagaagccc cataaatagt tctgtaagaa cacagtgagg
     9721 agacggtatc aggggcgagg aacctcccga ttcggttact tctgagcaag gatgcgtcag
     9781 gtggagtgaa gaaccccgga cgtcattccg acacaccaga acgcgccgag cgaatttgag
     9841 tacgcagatc cagaaggtcc gggatttcgg gcgccgcctg gtacgcctgg tctgcaaatt
     9901 cttcggcccg gataacatcc cctgaaaaga accagtacac tgccctgagt cccaggactc
     9961 ggggagggtc atacgcgtgc tcctcaagca cgcgcaggtg ctgatccgca tccgaaaggc
    10021 gaccggcgaa caggagccag aacgcggcgt cggcacggcg tttgtcctcc tcgctgccgc
    10081 cggtggacgc tcctaccgac gccaacaggt cgcgggcaac cggggaatcc ttccagagca
    10141 cagccaatgc actgtcctga agagttactc gccgttccag attccgggag tcaggaaatc
    10201 ggatctcctc gtccggggaa gcgtggaact caatgggttc gaacagcgcc caaccctccg
    10261 cgacacgggt gactcgaagg ttgccctgcg acgccagcga acgagcgacg cccgcggcat
    10321 tgctccacgc cgccatgaat ccctgcggga acaacgtcgt ctcaagggga agccaccacg
    10381 tgccctcaaa cgcgacgaac cgctccacgg gagccggtaa cacgtagggg gattcgtcaa
    10441 cggggaggcg ggtgtcgacc atcataaaga tgtgatccgg cgcttcgaca aacgcggttg
    10501 tgagccctgc gcactcgagg agcgaacaga gaaggacggt ggtgtcgtca cagtctcctg
    10561 cccgcaccct gagaagctcc gcgggataga ggatatcgtc cacaaccggg ttctggttga
    10621 gatctcccgc caacgcttga tacggaaaga gcagcagtcg gagcgactcc tcgccgatct
    10681 cccccacacc cgtgatcatt ccggagacac cggaaaccgc attcccaatg actccgagag
    10741 tgtctccgcg agcagaatcg gggccgctga tcacattcct gatcgggttc gcggcgatct
    10801 caacgggttt cacggcgagg gtaagcgtgt ttgcaccgat ccgcctcatc gcgtccaggg
    10861 gattctcagt ttttcgtacc tccgagaaag gtgtattggg atcgctctgg taggtgatcc
    10921 cgacctcgcc ggcggcttca aacaacacca tagctgtcgt gatgatttct tgagtgagtg
    10981 ggagctgttg cagacgcgac cggtagggcc ggagtgattc gcgcacgaac gcccggacat
    11041 ccgggtctgc cggtgtcaca aacgcggagg ccttcctcac gtcatcccat gtcagcgcgc
    11101 cgcggcgata caggaacgtg tccaccgtgt gtctgcgcca gacttctccg gtggccggat
    11161 tgacgaggct gatgaccccc tgaacgggtt gatcggcaac cgccgcgatt tgttcaccgg
    11221 agagaacagc gcgcagaaat atcctctgac gggagtacgg gcgcagtgtc acttcctcgg
    11281 aagacggtgc agtcatgaaa cgcggcagca ggagttccac gcgagcggtg acgggagtgg
    11341 aagaggagtt caccagttca atagaagcca cgggttcccg agtgtaggct ttgccgaggg
    11401 aggcgtagag agacctcacc tgtggcttcg taatctgcac cgcggagcgt cctctgggaa
    11461 acaccatcgc aaccgaaact ctgtgctggt ggtatgattg ctccagttgc gcgtacgcgt
    11521 agtcgaggcg aacactgcct gagcgaaagc cgactccccc acttcggctc cacatccggt
    11581 cacggtaccc gacacctccc ctcaacgcca tggattccac gggaaaccat tcaactccaa
    11641 tgcggtcgcc ctggtcgcgc tggtatgcga acgtcacctg atccgacgcg tcaaaggaga
    11701 cgccccagcg gacgctgcgc gggttcaggg tctcaacacg accatcttcg cgccacacct
    11761 tcgcgtccgt cagatcgtag atcgacgccc ccacgcgaat tcgccctgtc aacgaaaaca
    11821 gcgcaccgat gtccgtgctg aaccctgcag cctctcgcgc ggaatccggc gcagagcggt
    11881 actgcggagt gaaaaagctc cccgacatgc cgaccgcgag cttgtcggtc agacgaattc
    11941 ctacccccgc tgcaaaccga tccaggccct ccggggttcc tggatacggc aggatacgcc
    12001 tgttgcgaac ccaccccagc cctatcgcac cgtcctgtga gaccgggctt ccgaagaaca
    12061 gcgcgctgtt tcgaaggctc gtgctcccga ggtcacccgc aaggctggga aaaacctgct
    12121 cgaccaggcc cgccgggttc cacgcgaggg ccgcggggcc atccgttgag gaaacgatcg
    12181 cagtgcccat gccaagcgcc cgtgcgtctg tacaagggta cccgggatgg gttgtgctcc
    12241 acgtctgggc ttccaccctg atcgaagccc cagacacgag aagaaagcca acgaacactg
    12301 cgatttgcat cgccagcacc gttcacgaaa ggggttcgtt cagtatttct ttgaggacgt
    12361 tgggatggtt tgtggtggca ccgtccaccc ctgctgctat caacctccgc agtccgggtc
    12421 cggtatcgtc ggcgacaccc catgcacgca caagtaatcc cgctgcgtgg gctttgcgca
    12481 cgaactcgac atctatcagg gacgcacgag gcgctatcgt gtcgacgccc gcatccacgc
    12541 agcgattgat tgcctcgtcg ggaccgagat cctcgaaggt tgtcagccac gtcgttcgcc
    12601 agcgcggagc ttcgcgccgc accgtctcga ggacctccca ttcaaaggca ctggcaaccg
    12661 tccagccaaa cgcacaatgc cttgaaacca cggagaccac gccgggtacc gagtctttcc
    12721 caaccttcag ttcaatcacc aactgcgcct ggccggaaac cagcgtaaga agttcatcga
    12781 gaagggggac gcgctcgccg gagaacttcg gatccttcca ggaacctgcg tcaaggcgca
    12841 tgacctcgtt ccatgtcagg gagtcaaccc ggccagttcc gttcgtcgtc cgatcgacgg
    12901 tctggtcgtg aatgaccacg aaacaaccgt ctcgcgttct gcggacatcg cattcaagcc
    12961 acgtgcatcc cagctccaac gctgcccgga acgcgatgat cgtgttttcc gggtactggc
    13021 cgctgaaacc acggtgggca atgttcagaa atccctgttc catctcggca tccggatcag
    13081 agaatcaccg ggtcggacgg gttccgaacg tcttcgtcct tcttttccga ttcctccttc
    13141 gaggcagaca ccggcggggg cgggggagca ttcctcactg cgtcgcgtgt tccgttgatt
    13201 tcgtccgtca cgccggagat ttcgcggcgg aattcgcgaa tccctttccc gattccccgc
    13261 gccacctcgg gcagtcgttt tgacccgaag acgagaaacg cgacgagcaa aatgacgatc
    13321 atctcccagg gaccgacgtt cggcaacatg cagaatcctt tcctatccga ccggatcaga
    13381 ctgcgcgcca caggtggcgg aagaggaaaa tcgacccggc cacggcaaca ttgagtgact
    13441 ccgttcctct tttttgtgcc agagatacta tgcaatcaca taccacggtc caatcgcgcg
    13501 caacgccttc cgcttccccg ccgagaacca atgccaccgc atcccatttc aatagcagac
    13561 tggcaggttc tccgccgtgt gacgacgtgg ctacaagagg aatccctgcg ctccgtgccc
    13621 cacgaagaac gtcgtcaatt gagtgcgtgt acgaaacggg aagacggaaa aacgctccca
    13681 ttgagccgcg taccactttg ggattcgtca gctcaacggt gcccggcatg aggagtacgc
    13741 cgcgcgcccc cgctgcttcc gccgaccgca ggagggtgcc aaggttgccg gggtcgcgca
    13801 cgttgtccac cacaacaacg tccccggtgc ccgatttcca gacgtcttct tcggtccacg
    13861 tcggtgtacg agcgacggca acaacgccct gcggcgttcg cgtgtcgcag atcgcgtcaa
    13921 gttccttctg ggtaatgctc tgcgtcggga ttccatccga cgccgccgct tcaattaccc
    13981 gaaagagccg cgaatcggga tctgcctcga cgtggaacag gcattctacg gtaagcggag
    14041 aggcgaggac ctcttcaaca agccgaacgc cctccaccag aaacagcccg agagatttcc
    14101 ggttctcctt cgccagaagc ccacgcacgc gtttcagacc aattttcaat tctcatggcc
    14161 ctttttcgag gcatcgctca aggagtacac gagcacggtt ttgcgtggcc ttgaaatcgc
    14221 gcccataccc cacatgcgcc ggcgataaca cggcagcgcg aaggtctggc agcgcgtcgg
    14281 aacatttccc catctccgcc agtgcgatac ctcttctcgc tctcgcctcc acagaacccg
    14341 ggcactggaa ttggtccgca gaataaagtc caacggcctg tgtgtatgcc gccgctgctt
    14401 cctcccatcg ccgtagactg aacaacgcat cggcccgggc agtaacaaat gctcgattcg
    14461 ccgggtagcg tgccgcaatc tccgaggata tggccaacgc ttcgtggtaa ttcgcgtcct
    14521 tcagcaacgc ccagacaatc attccaggcg cggtgttctg cgttgtcgcc ccctccctcc
    14581 acgcccgttt catttcgcgt atgccctgac tccgcgtatc cgcgataaag ggcaaccatg
    14641 tccagttcct catcgctgct ccccgccagt aatgatacgc tccgagagcg agcagcgcgt
    14701 cagagaaacc gggttcacga cgcaatacct cttccagttg ttccacactc tcggtccccg
    14761 cgcggtacgc atgccagtac gagccctcgt ccgccgcaat gacggcgctc aggccgaggc
    14821 tgagtccgaa cacgtaccgg ggccagatcg cttccgggcg ccgcgcaacc tgctcacggc
    14881 ctgcgcggac tgcaatctga acacggcgtt tgagctcggc cggactcatt gttccctcgc
    14941 aaccgaaaag acgcatctgc tcaatggcag ccatggcata atcagccgtg gggtcagccg
    15001 gctgctcgcg cttcatgaca acagccaggc tctccgcgcg cgcgaactcc gaccgcagag
    15061 ctgcttcctg agcgagtacg aaattggcag gcacctgcac cgcgcgcaca gactccggca
    15121 gcgcggagaa aaacacgcac accgcgataa gctggcggcg caacccgacc gcgttgctac
    15181 acatccgctt catcaacccc agccatacgc ctgagggttg cggtttcttt ccccgcacgg
    15241 gcaccctatc tttgaaattg gtcggcgctt cggggaggct tccagaaccc gttcccaagg
    15301 gacgtatcac caccgccaac gacgtgattt caaatctgta cggtcagccg gaatgagaaa
    15361 gcctcttacc aagcggcaac tggcaattta tgaatatatt gccgcacaga ttcggcagcg
    15421 cggtattccc cccactatca gggaaatctg cgaccaattc ggcatgcgat cttccaacgg
    15481 ggcacgagag gcgctggcag cacttgcgag aaaagggtac atcaatcgac gagagcggct
    15541 ttcccgtggc atcgaactgg ccaacccgtt cgactctcta gagtgttcgg gaaccccaat
    15601 tcgtgtgcct cttttgagga atcccgttaa aggtgcccgt tgcattgacg aagcaacggg
    15661 tgagaaagtc ctgttcatcg attcgagttt tctgccgggc actggcagag cgtttgcctt
    15721 gctcgtaccc gacggttcgc ttgccaagta cggcatccga aaaggagacc tggcaatcgc
    15781 cacggaatgc tacgaagggc aaccgggcga aatcctgatc acggcaatcg actcgcgcct
    15841 cgtactcgtc cgcgttcctg cgacttccga ccaggagcct tctcagcagg ttccggaaag
    15901 ccacgggggt tcacccgtgt tcggaaaggt gaactgcgta gtgcgccgtt tcaccggcgt
    15961 ggagacgggg taggcccaac tgcgaatcgg cagatttgcg agagctttgt gcagcgcgga
    16021 caggtgtggt ctccgcaaaa cggagccaag gggtgggaaa catgcactgg cagttacgcg
    16081 cgcgttgggt atggtcccga ccgttccccg gtgagaaacg gtggggcacc catgcgctgg
    16141 ttccattact gaaaatcgct tttgtcttct ttcctgggct cctttttccc aggtgtaggt
    16201 acactgtagc gaacgcgtga caaaaagggt gtcgtcatga aaatcaacaa ggtagtgata
    16261 ccggttgccg gccttggaac gcggttactt cccgctacaa agtcgcaacc gaaggaaatg
    16321 cttcccgtgg gaagaaaacc ggttgtgcaa tacgttgtcg aggagatggt cgaacagggg
    16381 ttgcgctact tgttgcttat caccggcagc aacaagcact cgatagagaa ccattttgac
    16441 aacgacccca tgctcatcga gcgcttgctc agcaccgaag acgacgacct tcttcgggaa
    16501 gtggatctgc ggcccgaagg agcccagata ttctatacgc gccagatgat tcccccgggt
    16561 gcaaagaaac cggccggcct cggcgacgcc gtccgacacg cgcgggcgtt tgtgaatgat
    16621 gatccgtttg tggttgcgct gggcgatacg atcatccaca cggacggcaa accggacctg
    16681 atttcgcgca tgacccagac
//
LOCUS       DAOSOO010000052        16646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3493_length_16646_cov_5.533663, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000052 DAOSOO010000000
VERSION     DAOSOO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16646
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_16646_cov_5.533663"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1257)
                     /locus_tag="PLT86_05560"
     CDS             complement(241..1257)
                     /locus_tag="PLT86_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36111.1"
                     /translation="MTSRERVIRALRFEKPDRAPRDLWSLPGVAKYRNEELDRVCRLY
                     PSDFGGTGVRYGVSSRARPLQGDTGTYVDNWGCVWHVGEPGVVGEVKEPPIRTWSDLK
                     SYKLPWEIIREADFSRVNETCTAQQTFVRAGTETRPFERMQFLRGSSQLFMDLAYLEK
                     ELFQLRDMLHEFFCEELRLWTKTDVDAIQFMDDWGAQRALLISPDLWRSFFKPLYREY
                     CQIIKGAGKYVFFHSDGHIEAIYPDLIEIGVDALNSQLFCMNIEGLAAKYKGKITFWG
                     EIDRQYLMPFGTADEVRAGVRRVRRALDDGRGGVIAQCEWGVRDPIENVLAVFDEWEK
                     PTVA"
     gene            complement(1328..2476)
                     /locus_tag="PLT86_05565"
     CDS             complement(1328..2476)
                     /locus_tag="PLT86_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36112.1"
                     /translation="MEKLRVGVIGVGGMGTGHCEGLTKNVEETVLTAVCDIDPNVAKD
                     KAKTYGVKAFTSHKKLIKSGLVDAVTIATPHYFHSPIAVAAFEAGLHVLSEKPIAVTV
                     KAGDEMIAAAKASGLVFGVMFQQRTRPVSQAMKNIVSSGALGRIYRAMMVETHFRTQG
                     YYDSATWRATWKGEGGGVLLNQAPHGIDIFTWLMGLPKKVKAVTRTWRHRIEVEDEAS
                     ALLEWENGAVGYYHTSTNESPSSDILEICGDNGKLVYNHGKASLFLNDPPIQKFSDDA
                     GIMWGNPKVTEQPIELIDRPTGHSAILRNWARAILYGEELIAPGVEGINSLEFINAVI
                     FSGATGKEVELPVPRAAYERWIKTKARTSTFQKHVVEKRETDPQHVGK"
     gene            complement(2489..3244)
                     /locus_tag="PLT86_05570"
     CDS             complement(2489..3244)
                     /locus_tag="PLT86_05570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT36113.1"
                     /translation="MATLSSFRWPNGARGAVSLSFDDGLRTHVENAIPTLNDYRFRGT
                     FYIPMVRGGAFDQRIDHWRAAASQGHEIGNHSMTHPCSANFGFSRRALDYLTLEDITE
                     EILDAKRRITEDIPDQKAHSFAYPCGETKVGSGKSHVSYVPVVAEHYVVGRGIGDFAN
                     DPVLCDLACTWSFMPTDPPAERLIGWADMARNMGRWSIFCFHGVGGEHFHLRMEAFRM
                     LLDHLAAHRDQIWTDTVINVGTWIRDHRERVCA"
     gene            3434..4459
                     /locus_tag="PLT86_05575"
     CDS             3434..4459
                     /locus_tag="PLT86_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36114.1"
                     /translation="MNHKERLLAAIDHRETDRTPIWLGCPHGDSHARLHKYFGVNSNL
                     ELQTAIDDDMRWFPIGWKHPEGKPGLDPTGGRPKKSHNQPGIFAETEDVGEVEKAYWP
                     DPQYADVAGARDAIDPWKHTHAIFSGVWAPFFHNVGDYFGMDNYFVKMHTNPSVVEAV
                     TERVVDFYWGVNEIAFSHLASDIDVMFFGNDFGTQLNTFVSPELFRRFILPGMKKLTD
                     QAHRFGLRVALHSCGSVYGVIPDLIGIGVEALHPIQGKARNMEAVRLKREFGNDLCFI
                     GGIDTQELLCNGTPQQVRDEVRRVIDIFGTGIVISPSHEAILPDVPPENVDAMFDEAR
                     TYHAKPR"
     gene            4480..4905
                     /locus_tag="PLT86_05580"
     CDS             4480..4905
                     /locus_tag="PLT86_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT36115.1"
                     /translation="MAFPTHYETRFDWSGSGENGLAAGEKLAPLPVGTPSAGEGRWNP
                     EAMLLSAVETCLFNTFAYIAAMSKLSFRSYHSTASGDVELVPREGYRFARITVRPVIA
                     VSGKDIARAQRILEKAHEACLISRSVNFPIDIEPTIVEE"
     gene            5036..5776
                     /locus_tag="PLT86_05585"
     CDS             5036..5776
                     /locus_tag="PLT86_05585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT36116.1"
                     /translation="MKAEIRGFVKLVHDTAALLRVREIRTLAVGTLILLLWQYFGYPF
                     TYSEMIADKTGEWRYDLIAPNLYWTATNVILLLLLPLALEASQAKGSLRSFGLGIGDW
                     RLGCAASVGGYVIMVPFIALAASRPDFQATYPLNQNSLLSHELFWVYEASYLLFFVGW
                     EFFFRGYWLFTLEPALGMAAVAVQTVPFALMHVGKPFPEAIGSVFAGLFLGMLALRTR
                     SIWYGVFLHAAVAGTMDIIVYLQRGIGK"
     gene            complement(5830..7029)
                     /locus_tag="PLT86_05590"
     CDS             complement(5830..7029)
                     /locus_tag="PLT86_05590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT36117.1"
                     /translation="MPAPIFNRRVSAIEESITLAVTAKASEMKSKGIDVVSMSAGEPD
                     FDTPDHIKYAAIKAITEGFTKYTPVGGTMELRKAVAEKFARENGLTYAANQTLVSIGG
                     KHSCYLAVAALVDDGDEVLIPAPYWVSYPDMVLLAGGKPVIMETSTATGLKITPDQLR
                     KAITPRTKMIIINSPSNPSGAVYTKDELVALGDVLKGTGVFVLSDEIYEHLIYEGEHH
                     SIAAVVPELSEQTIVANGVAKAYAMTGWRIGYAAGPKSVIGAMAKIQSQQTSNAVSIS
                     QKATLAALTGPQDHLVPWKEEYRTRRNYIVERLNAIPGVLCPTPAGAFYVFPDVSSFY
                     GKSFGGRVIEDSVALTDYLLEEFHVACVPGSGFGAPRHIRLSYATSMAAITKAMDRIE
                     KGLTALS"
     gene            7316..10894
                     /locus_tag="PLT86_05595"
     CDS             7316..10894
                     /locus_tag="PLT86_05595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT36118.1"
                     /translation="MRRSDFVHLHAHSAYSLLDGVSQPRKLVQRALSLGHEALALTDH
                     GAMYGTVEFYQAAKDAGIQPIIGCEVYVAARSRRDKEAQRDRKSHHLVLLARTNEGYK
                     NLIQLVTKAHMEGFYYYPRVDHELLHRFGSGLVCLSGCLSGEIPDAITSGDMDGARAL
                     IEAYHSHFGAGNFFLEVQHHPDLPSQEKVNQGVFELAEEFGIPVVATNDSHYACTTDA
                     EAQDALLCVQTSATLDQADRMSMMDGDYSLLSTEEMSSFFDGMPETITNTLRVAEMCN
                     VEIELGKARLPKFPTPKNLSPSEYLRELCEEGLRKRYPLEKAQDGQWRLREGHAEAEL
                     PVPLPEILERLDYELGVIGRMGFDSYFLIVWDYVQYAKKNGIVVGPGRGSAAGALVTY
                     VLGITELDPLRYDLLFERFLNPDRISMPDADVDFDDEHRDQVVEYVRQKYGRDCVANV
                     ITFGTMQAKGAIKDVGRAMGMTFDETDRIAKLVPNRVGVELSECLESEPEFRKAYEEN
                     PRNKKLVDLAMQLEGVTRHVSVHACAVVISDIPLENFTPLQYAPRSEDVIITQYEAHA
                     LESLGLLKMDFLGLRNLTVAKRATELIKRNHGVDVDLYNLPFDDPATYELLSRGETTA
                     VFQLESSGMKRYLRELRPNRFEDIIAMVALYRPGPMQFIEEYCARKHGRAPIIYDHEL
                     MVPALESTYGITVYQEQVMQMSRELGGFTRGEADTLRKAMGKKIGSLMDQMKGKFVSG
                     ARERGIGEELATKIWGDWEKFASYAFNKSHAACYAFVAFQTAYLKTHYPYEFMAAALS
                     SEIGDSKRIIVLSDECRRMGVPLLPPNVNKSELQFTVSKEGIRFGLLAVKNVGIHAIE
                     AITHAREQEGPFSSLFDFCRRVDNKAINKRMIESLIKSGAMDDLGERAQMLAGLDAAI
                     NWAQNEQENRSRGQTSLFDAGSSDVAHGPAIEETTPTLPSVAPLSVQEKLEQERELLG
                     FYVSGHPLDPHRVDIELFCSHSSEALGELGGDTPLLIAGIIRSIRRITTKAQKQMAVL
                     VCEDFSGTFEVVVFPDVYEAAQDYLIDDQIVFVRGTRSRDEPKVMALEVIPITHARMR
                     LAKAVDVSVASEKLTDDAVGELRRIIKAHGGTLPVYIHVTTKSHGRLRFRARSGAMNP
                     TNDCLAELGKQPAVESVTLCPAVWEVPQPPRRGYGEGNGAAAAGYR"
     gene            10949..12427
                     /locus_tag="PLT86_05600"
     CDS             10949..12427
                     /locus_tag="PLT86_05600"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT36119.1"
                     /translation="MRSSITVRPDSAYGVVRPELYGHFAEHLGRCIYEGIWVGEESPI
                     PNDGGIRCDTVNALKRIRPPILRWPGGCFADVYHWEDGIGPRDQRPRRPNHWWEGQDT
                     NAFGTHEYLETCERLGAEPFICLNVGSGTPREALNWVEYCTYSGDTEYTRKRARNGHP
                     LPWNVRYWAVGNENWGCGGKFEARDYAREYRRFVSFLKQTAPQGEFVICGFDQQKWNL
                     DVLDELRDDLGYIDHISIHRYVSRGTGSGFTPEEYWLLLKDVSALEDVILMTSGTVQS
                     FTQYSKRYIGIALDEWGVWHRDATVDRGLSQPNTLRDAIFAASAFHMFQRHASILSMT
                     NIAQTINVLQCLLATDGDKLALTPTYYVYDLLQPHKGATAIVTEVDGPSVEVTDAGRR
                     STLPVLDASATASPDGTMTISVINRHPSEAQEGCVKVAGSTSFPNISARCLGGTSPGA
                     ENKPGATESVAPAEVSTAPVKNGEFVHTFPPCSVTVFRLSHV"
     gene            12551..13516
                     /locus_tag="PLT86_05605"
     CDS             12551..13516
                     /locus_tag="PLT86_05605"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36120.1"
                     /translation="MTLPDASCEPIVLAYYYIWFTRGWFDGSHTPQYKATMADIHPAI
                     GSYDSHDPDVISEHMKQCVKAKLDALAVSWFYQKRNLTGATSDDILESVMTAASDHGL
                     RTCIDMEAAGLCADRLYEALKHYLSRYSNDPRALHAQGKPVVLCWATWKLGADVWTQI
                     TKRLREQGMDAFHVASNQMDPSYFSFLDALECYTPLSPEQTVMEQYTGARAAVDAYNE
                     TPAGRTRPGRWHATVMPGFEDRLMTDRLKRDDYAHFRMRRSGYYYWETFAAAMASRPE
                     WLHVTSFNELAEHSHIEETVEDGDLYLRLTAEFATHFKAARTAWK"
     gene            13559..14641
                     /locus_tag="PLT86_05610"
     CDS             13559..14641
                     /locus_tag="PLT86_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36121.1"
                     /translation="MPRGQNLGTERIDRRDADTGLLVRQITSFPTPSTHLHYETPTFT
                     PDGRRMIFLSMRVPERGAPSDLFTAGSDGKDIVQLSDDHPSGVSNPTLAKDGQSAYYT
                     HGGTAYRTRLENLETVELGHVPGASQHAYPRAMCSWDGRWYFAMARLANGRIGLVRWD
                     AGTGEHVVVLEADGINHPRANPGGPEIDCSVKTRRPDGSIESRKVRFHCETLKEMNAD
                     FPTGEFKTAHSCWLGATGDYQGTLQMPHRGVIVMRRGAAEPEKVAFGGPYFWHSGASN
                     DGKWIIADTNWPDEGLWLFNVGTKKRERICFAGASQGHPQSTHTHPNLSGDGAMAVFT
                     SDRTGMPQVYVAYIPEEMRDRLARAD"
     gene            complement(14771..16411)
                     /locus_tag="PLT86_05615"
     CDS             complement(14771..16411)
                     /locus_tag="PLT86_05615"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOT36122.1"
                     /translation="MANFPAVRSWRAALTAPSPALINRVARMRNQSPSEAGSAIRWYA
                     TLVERAQSVFGHNDDVPMFIVRAPGRVNLLGMHIDHRGGRVNPIAVRELVLVCVPRSD
                     NRIRIANVNPSFPADEFAVPELLPGNKITDWADWTLTTPETLRQKGLLGHWSSYVRSA
                     AAYFANSWGMRDSIRGLDILGDSQLPQAAGLSSSSALVVAAGYALHLANEKPIDRRAL
                     AEGCGQAEWYVGTRGGFGDQAAIALSRSGHVSHVDFFPMNVDWSPWRPGVSVVVCHSR
                     VHAQKTANARSVFNERVATYVIALLWIKKARPEWQGRLEYLRDVLRLGVHVSEIYRVL
                     KQLPVRATREQIHSALPGAREELEKLFSTHDDPAEGYRVRDVCLYGLAECERSLILAD
                     FLRRGDIAGAGRLMDLSHEGDRVTVLDDSGVRVPYDSGVSDADLDGLIEASQSPDPEI
                     REAARLALQPGGYAASCAELDEIVDIARSVDGVYGAGLIGAGLGGCVEAIVDDGAVEN
                     LRNALITQYFEKHERDPFIEVMAPVEGACAVELPEFQQ"
     gene            complement(16488..16625)
                     /locus_tag="PLT86_05620"
     CDS             complement(16488..16625)
                     /locus_tag="PLT86_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOT36123.1"
                     /translation="MKLVELDRFAFYDFTKEKAFAIVMTGETALYANLILVKGVVAPK
                     K"
BASE COUNT         3491 a         4874 c         4838 g         3443 t
ORIGIN      
        1 ggttttccgc ggacgagtgc gttccatttt ctggcgacga tctgggctgg cggccaacct
       61 ggacgagcgg aaaaggggtg aactcgttgg cgggacagat gattcgtctg cgtgtatccg
      121 cgcggcgcgc ttcgcttttt gcgatgactt tgtagcagaa cctaaccttt ttcttctggg
      181 ggaaaaagag gcccgccgaa cacagcgggc ctttttcttt ccatgccgcg gttcagtctt
      241 tcacgcgacc gtcggcttct cccactcgtc gaacaccgcc agaacgtttt caatcgggtc
      301 tcgcacgccc cattcgcact gggcgatcac gccgccgcga ccatcatcca gagcccggcg
      361 gacgcgcctc acccctgcgc ggacttcgtc cgcggtgccg aagggcataa ggtactggcg
      421 gtctatctcg ccccagaagg tgattttgcc tttgtatttc gcggccaggc cctcgatgtt
      481 catgcagaaa agttgcgagt tcagcgcatc cacgccgatt tcgatcaggt ccgggtaaat
      541 cgcctcaatg tgcccgtcgg agtggaagaa tacgtacttg cccgctcctt tgatgatctg
      601 gcaatactcc cggtacagtg gtttgaaaaa agagcgccag aggtctggag agataagcag
      661 cgcacgctgc gcgccccagt cgtccatgaa ctggatcgcg tccacgtccg tcttcgtcca
      721 gaggcgaagc tcttcacaga agaactcgtg cagcatgtca cgaagctgaa aaagctcctt
      781 ttccaggtac gccagatcca tgaaaagctg ggagctaccc ctgagaaact gcatgcgttc
      841 aaagggtcgg gtttctgttc ccgcgcggac gaacgtctgt tgggccgtac atgtctcgtt
      901 caccctgctg aaatcggcct cccggattat ctcccacggg agtttgtaac ttttgagatc
      961 actccaggtt cgaatcggcg gttccttcac ctcgccgacg actcccggct ccccaacgtg
     1021 ccagacgcag ccccagttat ccacgtaagt tccggtatcc ccctgaagcg gacgagcgcg
     1081 ggagctcacc ccgtaccgaa cgccggttcc accgaaatcc gacgggtaca gcctgcatac
     1141 ccggtcgagc tcctcgttcc ggtacttcgc gacccccggc agcgaccaca aatcgcgcgg
     1201 tgcccggtca ggtttttcga atctcagcgc tcggatcact cgttcacgcg aagtcatatc
     1261 tgattgcttc tcccggggga aaccggggga gcgtgaactc ccccggctgc taaccgtccg
     1321 gaaccgatca tttccctacg tgctggggat cggtttcccg cttttccacc acatgttttt
     1381 gaaaggtgct cgtacgagct tttgtcttta tccagcgctc gtacgcggca cgcggaaccg
     1441 gcagttccac ctctttgccc gtggcgccgg agaaaataac cgcgttgatg aactcgaggc
     1501 tgttgatccc ctccacgccg ggagcgatga gttcttctcc gtacaggatg gcgcgggccc
     1561 agttccgcag gatcgcgctg tgcccggtgg ggcggtcgat cagttcgatg ggctgctcgg
     1621 ttactttcgg gttgccccac atgatgcctg cgtcatcgct gaatttctga atcggcggat
     1681 cattcaggaa aaggctcgct ttcccgtggt tgtacacgag tttcccgttg tcgccgcaga
     1741 tctccagaat gtccgaggac ggagattcgt tcgtgcttgt gtggtagtac ccgacggcac
     1801 cgttttccca ttcgaggagc gcactggctt cgtcttcgac ttcgattcgg tgcctccacg
     1861 tccgggttac ggctttcacc ttcttcggca agcccatcag ccacgtgaag atgtcgatac
     1921 catgtggagc ctggttcagc agcactccgc cgccctcccc tttccacgtt gcgcgccacg
     1981 tggcggaatc gtagtagccc tgcgtccgga agtgggtttc caccatcatc gctcgataga
     2041 tacgtcccag cgcgccgctt gaaacgatgt tcttcatcgc ctgactgacc ggacgcgtgc
     2101 gctgttgaaa catgacaccg aataccagcc cgctggcttt cgccgctgca atcatctcgt
     2161 caccggcttt gacggtcact gcgatcggtt tttccgagag cacgtgcagg ccggcttcaa
     2221 acgccgcaac cgcaatgggg gagtggaaat agtgcggagt tgcgatggtc acggcatcca
     2281 cgaggccgct tttgatgagc ttcttgtggc tggtgaaggc tttcacaccg tacgtcttcg
     2341 ctttatcctt cgcgacgttc ggatcgatgt cgcagactgc cgttagcact gtttcttcga
     2401 cgttcttggt caatccttcg cagtgtccgg tacccatacc gccgacaccg atcacaccta
     2461 cccggagttt ctccattttg cattctcctt atgcgcaaac gcgctcgcga tggtcgcgaa
     2521 tccacgttcc cacgttgatg acggtatccg tccagatttg atcgcggtgc gctgccagat
     2581 gatcgagcag cattcgaaaa gcttccatcc gcaggtgaaa atgctcccca ccaaccccgt
     2641 ggaagcagaa aatggaccac ctgcccatgt tcctcgccat atccgcccac ccgataagcc
     2701 tttccgccgg cggatcggtc ggcataaagg accacgtaca ggcgagatca cagaggacgg
     2761 ggtcgttggc gaaatctccg attccgcggc cgacaacgta atgctctgct acgacaggca
     2821 catagctgac atggctcttc cccgacccta ccttcgtttc accgcacggg taggcaaacg
     2881 aatgtgcctt ctggtccggg atatcctctg ttatccggcg ttttgcatca aggatttcct
     2941 cggtgatgtc ttccagcgtg aggtagtcca gagctcgccg gctgaatcca aaattcgcag
     3001 aacacgggtg tgtcatgctg tggttaccga tttcgtgccc ctggctcgcg gccgctctcc
     3061 agtggtcaat tcgttgatcg aacgcccctc cgcgcaccat cggaatgtag aacgtgccgc
     3121 ggaacctgta gtcgttcagc gtcggaatcg cgttctccac atgggttctg aggccgtcgt
     3181 caaaggaaag ggagaccgcg cctcgtgctc cgttgggcca tcggaaggaa ctcagtgttg
     3241 ccaaagggtg cattcccggt ttgatgaaac aaggtgcgtt gaacccgtga cagaccgaaa
     3301 gaagagtata cgccaccaga cggagcgctc caatcgcagc gcgtttcgcc ttacagactc
     3361 ccttccgcat tggggactcg agggcgatac tttggtacgc aatggtctct accagatgcc
     3421 gggaggctga ataatgaacc acaaagagcg cctgcttgcg gcaattgatc acagggagac
     3481 ggacagaaca ccgatttggc tcggatgtcc tcacggagat tcccacgcca ggctccacaa
     3541 gtatttcgga gtcaactcaa atcttgaact gcaaacagcg attgacgatg acatgaggtg
     3601 gttcccgatc gggtggaagc accctgaggg gaaacccggc ctggatccaa caggagggag
     3661 acccaaaaag tcgcacaatc agccggggat cttcgcggag actgaggacg tgggcgaggt
     3721 ggaaaaggcg tactggcccg atccgcagta tgccgacgtt gccggcgcgc gagacgccat
     3781 cgatccgtgg aagcatacac acgcaatttt ttcgggggtg tgggcgcctt ttttccacaa
     3841 cgtgggcgac tacttcggca tggacaacta cttcgtcaaa atgcatacca acccgtctgt
     3901 tgtcgaggcg gtaaccgaac gcgtggtgga tttctactgg ggcgtcaatg agattgcgtt
     3961 ctcgcatctg gcgtcagaca ttgatgtgat gttttttggc aacgatttcg gaacgcaact
     4021 caacacattc gtaagccccg aattgttccg caggttcatc ctccccggta tgaagaagct
     4081 gaccgatcag gcacaccgct tcgggttgcg agtggcgtta cactcctgcg gttccgtata
     4141 cggcgttatt ccggacctga tcgggatcgg cgtggaggca ctccacccca tccagggcaa
     4201 agctcgcaat atggaggcgg ttcggctcaa acgcgaattc ggaaacgatc tctgtttcat
     4261 tggagggatc gacacgcagg agctgttgtg caacggaacg cctcaacagg tcagggacga
     4321 ggtgagacgt gtcatcgaca ttttcggcac tggcattgtg atttcgccga gccacgaagc
     4381 catactcccc gacgttcccc cggagaacgt ggacgcgatg tttgacgagg cccggacgta
     4441 ccacgcgaaa cctcgataga aacagaaagg cccttatcga tggcattccc tactcattac
     4501 gaaacacgtt ttgattggag cggttccggg gagaatggtc tcgcggcggg cgagaagctg
     4561 gctcccttgc cggtgggcac accgtccgca ggagagggca ggtggaaccc cgaggcgatg
     4621 cttctttcgg ccgtagaaac gtgtctgttc aatacgttcg cgtacatcgc ggcgatgtcg
     4681 aaattgagct tccgcagcta tcactcgacg gcatcaggcg atgtggagtt ggtgccgcgg
     4741 gaaggctacc gtttcgctcg tatcactgtg cggccggtga tcgctgtatc cggcaaagac
     4801 atcgcccgtg cacagcgcat ccttgaaaag gcgcatgagg cgtgcttgat atcgcggtcg
     4861 gtgaattttc cgatagatat cgaaccgact atcgtagagg aatagtcggc cgcaaacttc
     4921 ctgcgaaagg aatctcgccc gctccgatgt cgcggccgca tggcagaccc cagttgacac
     4981 agaagttgca cggcaccggt ccgcgttcgt cgaacgaacg atcagaaagg caggaatgaa
     5041 ggcagaaatc cgcggcttcg tgaagcttgt ccatgatacg gcggctcttc tgcgagtgcg
     5101 cgaaatccgc actttggccg tggggaccct gattcttctt ctctggcaat acttcggcta
     5161 cccgttcacg tactccgaga tgatcgccga caaaacgggg gaatggcgct acgatctgat
     5221 cgcccccaac ctctactgga cagcgactaa cgtaatcctt ctccttctcc tgccgttggc
     5281 ccttgaagcg tcgcaagcga aaggcagtct gcgttccttt gggttgggga ttggcgactg
     5341 gaggctcggg tgtgccgctt cggttggtgg gtacgttatc atggtgccgt tcatcgcgct
     5401 ggccgcctcg cggccggatt tccaggcaac gtacccgttg aatcagaatt cgctgttatc
     5461 tcacgaatta ttctgggttt atgaagcatc ctatctgttg ttcttcgtcg ggtgggagtt
     5521 ctttttccgc gggtactggc ttttcacact ggaaccggca cttggcatgg cggcagttgc
     5581 cgtccagaca gtaccattcg cgctcatgca cgtgggcaaa ccgttccccg aggctatcgg
     5641 ttccgtcttt gcaggtttgt tcctcgggat gctcgccctg cggacacggt ccatctggta
     5701 cggggtcttt ctccacgccg ccgttgccgg gacgatggac atcatcgtgt acctgcagcg
     5761 cggaatcgga aaatgaacgc gggagtgccg acgtcggcac tcccgcgaag tacagccgca
     5821 gcgctcaggt caggaaaggg ccgtcagtcc tttctcaatt cgatccatcg ccttcgtaat
     5881 cgccgccatt gaagtggcat acgagagacg gatgtgtcgt ggcgcgccga aaccgctgcc
     5941 aggaacgcac gcaacgtgga actcctcgag gaggtaatca gtcaacgcaa cgctatcttc
     6001 tataaccctg ccgccgaacg acttaccgta gaacgaagag acatccggga acacgtagaa
     6061 cgcacctgcc ggtgtcggac aaagtacgcc ggggatggca ttgagccgtt ccacaatgta
     6121 gttgcggcgc gttctgtatt cctctttcca ggggacgaga tggtcctggg gccccgtaag
     6181 cgcggccaga gtagccttct gtgaaatcga gacggcattc gaggtctgct ggctctggat
     6241 tttcgccatt gcgccgataa ctgattttgg acccgctgcg tacccgatgc gccaccctgt
     6301 catggcgtat gcttttgcca caccgttcgc cacaattgtt tgctcgctca actccggcac
     6361 gactgcagcg atggaatggt gctcaccctc gtagatcagg tgctcgtaga tttcgtccga
     6421 aagcacgaac acccccgtgc ctttcaggac gtcacccagt gcgaccaact catccttcgt
     6481 gtagactgcg cccgatgggt tcgaagggct gttgatgata atcatcttgg ttcttggcgt
     6541 gatcgccttc cgcaactgat ccggagtgat cttgagcccc gttgccgtgc tcgtttccat
     6601 gattaccggc tttcctccgg caagcaggac catgtcgggg tagctcaccc agtacggagc
     6661 cggaatcagg acctcgtcgc cgtcgtccac gagcgctgca accgcgagat aacacgaatg
     6721 cttgcctccg atactgacaa gggtctgatt cgcggcgtat gtcagaccgt tttcgcgggc
     6781 gaacttctcg gcaacggctt tcctgagctc catcgttccg ccgaccgggg tgtatttggt
     6841 aaaaccttct gtgatcgcct ttatggcggc atatttgatg tggtccggcg tatcaaaatc
     6901 gggttcgccg gcgctcatgg ataccacatc aatgcccttg gatttcattt ccgaagcctt
     6961 cgcggttacg gcaagagtaa tcgattcctc gatggccgag acgcggcggt tgaaaatcgg
     7021 agctggcatg ctggcatcct ttcgttgggc acagagtggt gtcagaggac gttgcgtgtc
     7081 cgaactaaaa gtaccggagg caaggagcga caccaagggc aggtttcctc cagtccggct
     7141 ggccgcgctg ctttgcacgg ctggtttcgg gcatgtggcg cgaacaggca aatcagcgcc
     7201 gagttgtccc gtggtccctt gaccagagga agattcacgc ggagttttca gaaaaccgtg
     7261 tgtggagttc ctccccgcct tttgcacgaa accatctgcc cctcggagta acgctttgcg
     7321 gagatcagat ttcgttcatc ttcatgcaca ttcggcatac agcctccttg acggtgtatc
     7381 gcagccccgg aaactcgttc aacgcgccct ctcgctcggt cacgaagccc tcgcgctcac
     7441 tgaccacgga gccatgtacg gcacggtgga gttctatcag gcagccaagg atgcgggtat
     7501 ccagcccatt atcggatgtg aagtttacgt ggcggccaga agcaggcggg ataaggaagc
     7561 gcagcgtgat cgaaaatctc accatctggt attactggcg cgaacaaacg aggggtacaa
     7621 gaacctcatt cagcttgtga caaaggccca catggagggg ttttactact acccccgcgt
     7681 tgaccatgag ctcctccaca gattcggctc cggccttgtc tgtctttccg gctgtctttc
     7741 aggggaaatc cccgatgcta ttacgtccgg cgatatggac ggtgcgcgcg cgctcattga
     7801 agcctaccat tcgcatttcg gcgcgggcaa cttcttcctt gaagtccagc accaccccga
     7861 cctgccctcc caggaaaagg tgaaccaggg cgttttcgag ctcgctgagg agttcgggat
     7921 cccggtagtc gccacgaatg acagccacta cgcctgtacg actgatgccg aagcacagga
     7981 tgcgctcctt tgcgtccaga cctcggctac actggaccag gctgaccgca tgtcaatgat
     8041 ggacggtgat tacagcctcc tttcaaccga ggagatgagc agctttttcg acgggatgcc
     8101 ggagacgatt acgaatactc ttcgagtggc ggaaatgtgc aacgtggaga tcgaactggg
     8161 gaaagcacgc ctgcccaagt tcccgacgcc gaagaacctg tcgccctcag agtacttacg
     8221 ggagctttgc gaagagggtc tgaggaagcg gtatccgctt gaaaaggctc aggacgggca
     8281 atggcgcctt cgtgaggggc acgctgaggc ggaattgccg gttccattgc ccgaaatcct
     8341 ggaacgcctt gattacgaac tgggcgtgat cggcaggatg gggttcgatt cctatttcct
     8401 catcgtatgg gattacgtcc agtatgcgaa aaagaacggc atcgttgtgg gcccggggag
     8461 gggttccgcg gcaggcgcac ttgttacgta cgtgctggga atcacagaac tggaccctct
     8521 ccgatatgat ctcctgttcg agcgcttcct gaatccagac cgcatctcca tgccggacgc
     8581 agacgtggat tttgatgacg aacatcgtga tcaggttgtg gaatacgttc gccagaaata
     8641 cgggcgcgat tgcgtcgcca atgtgatcac cttcgggacg atgcaggcga agggcgcgat
     8701 caaggacgtc gggcgcgcga tgggcatgac gttcgacgag acggaccgga tcgccaagct
     8761 cgttccaaac agagttggcg tggagctttc cgaatgcctt gaatcagagc cggagttccg
     8821 caaggcgtat gaagagaacc cgcgaaacaa gaaactcgtc gacctggcga tgcagcttga
     8881 gggggtaacc cgccacgtat cagttcacgc gtgtgcggtg gtgatttcgg acatccccct
     8941 cgagaacttc acccccctgc agtacgctcc acgaagcgaa gacgtgatca tcacccagta
     9001 cgaagcccac gcgctcgaat cactcggcct cctgaaaatg gacttcctcg ggcttcggaa
     9061 cctcacggtg gcgaagcggg caaccgaatt gatcaagagg aaccacgggg ttgacgtgga
     9121 cttgtacaat ctgcccttcg acgaccccgc cacgtacgaa ttactctcgc gcggcgaaac
     9181 gaccgcggtc tttcagctcg aatcctcggg aatgaagcgg tacctcaggg aactccgccc
     9241 gaaccgattt gaagacatca tcgcgatggt ggctctgtac cgcccgggac cgatgcagtt
     9301 catcgaggag tactgcgcaa ggaaacatgg ccgggccccg atcatctatg accacgaact
     9361 gatggtgccg gcgctcgaaa gcacgtacgg gatcactgtc tatcaggaac aggtgatgca
     9421 gatgtcgcgc gaactcggcg gattcacgcg cggagaagcg gacacgctgc ggaaggccat
     9481 gggcaagaag atcggttcgc tcatggacca gatgaaaggg aaattcgttt cgggagcccg
     9541 tgagcgcgga atcggcgaag aactcgccac gaaaatctgg ggcgactggg agaaattcgc
     9601 ctcctacgca ttcaataaga gccatgccgc ctgttatgca tttgtcgcgt ttcagactgc
     9661 atacctgaag acgcactatc cttacgaatt catggcggct gcgctttcgt cggaaatagg
     9721 ggattcgaag cggatcatcg tgctcagcga cgaatgccgc cggatgggcg ttccgcttct
     9781 gcctccgaac gtgaacaaga gcgaactgca attcaccgtc agcaaggaag gaatccgttt
     9841 cggacttctc gccgtcaaga acgtgggtat ccacgcaatc gaggcgatca cccacgcacg
     9901 tgagcaagaa ggcccgttct cctcgttatt cgatttctgc cgtcgggtgg acaacaaggc
     9961 gatcaacaag cgcatgatcg agagcctcat aaagtcgggc gcaatggatg acctgggaga
    10021 acgggcacag atgctcgcag ggcttgatgc ggcgatcaat tgggcacaga acgagcagga
    10081 aaaccgatcc cggggacaga catctctctt tgacgccggg tcgtctgacg tcgcgcacgg
    10141 ccctgcgatc gaggagacaa cgccaactct tccttctgtg gcgccgctgt cggtccagga
    10201 aaagcttgaa caggaaagag aactgctggg cttttacgtt tcaggccacc ctcttgaccc
    10261 gcatcgggtt gacattgagt tgttctgcag ccacagcagc gaggcgctgg gggaactggg
    10321 cggcgacacg ccgcttttga tcgctggaat cattcggagt atccgccgta tcacgacgaa
    10381 ggcgcagaaa cagatggcgg tccttgtgtg cgaggatttc tccggcacgt ttgaagtggt
    10441 cgtgttcccg gacgtctacg aagccgcaca ggattacctc atagacgacc agatcgtgtt
    10501 cgtgcggggc accagatcgc gcgacgaacc gaaagtgatg gcgctcgagg tgatcccgat
    10561 cacccatgcg cggatgcgcc tggcaaaggc agtggatgtc agcgtcgctt cggagaagct
    10621 cactgacgac gcagtgggag aactccgacg tatcatcaaa gcccacggcg gaacactgcc
    10681 cgtgtacatt cacgtaacca caaaatccca cggcaggttg cggttccgcg ctcgatccgg
    10741 cgcgatgaac ccgactaacg attgtctggc ggagctggga aagcagcccg cggtggaatc
    10801 ggtaacgttg tgccctgccg tgtgggaagt accccagccg ccgcgtcgcg gttacggtga
    10861 gggcaatggc gcggcggcgg ctggctaccg ctaacctttc ccggaatcag aggcgcagta
    10921 gatccactct gcgcgggaga catactcgat gaggtcatcc atcacggtga gaccggactc
    10981 cgcgtacgga gtcgtgcgac cagagctgta cggccatttc gctgaacatc tcgggcgctg
    11041 catctacgag gggatctggg tcggcgagga gtcccccata cccaatgacg ggggcatacg
    11101 gtgcgatacc gtcaacgcgc tcaagcggat aaggccgccg atactgcgat ggccgggtgg
    11161 ctgtttcgcc gatgtgtacc actgggagga cgggatcggg ccgcgtgatc agcgccccag
    11221 acgtccaaat cactggtggg aggggcagga tacgaatgcg ttcggcacgc acgagtatct
    11281 ggaaacgtgc gagagactgg gagcggaacc gttcatctgc ctcaacgtcg gaagcggtac
    11341 cccgcgcgag gcactcaact gggttgagta ctgcacctac tccggcgata cggaatacac
    11401 acggaaacga gctcgaaacg ggcatccact accctggaac gtccgttact gggcagtcgg
    11461 gaacgaaaac tggggctgcg gcgggaaatt cgaagcacgg gattatgcac gcgaataccg
    11521 gcggtttgtg agcttcctga aacagacggc gccacaggga gagttcgtga tctgcggatt
    11581 tgaccagcag aaatggaacc ttgatgtgct ggatgaactg cgtgacgacc tcgggtacat
    11641 cgaccacata tcgatccaca gatacgtctc tcgcggcacg ggatcggggt tcactccgga
    11701 ggaatactgg cttcttttga aagacgtctc cgctctggaa gacgttattc tcatgacgag
    11761 cggtactgtc cagtcattca cccagtactc aaagcgctac atcggcatcg cgctcgacga
    11821 gtggggcgta tggcatcgcg acgccacggt agaccgtggg ttgtcccagc cgaatacgct
    11881 gcgggatgcg atttttgcgg cgtcggcgtt ccacatgttt caaaggcacg ccagcatcct
    11941 cagcatgaca aacatcgcgc aaaccatcaa cgtgcttcag tgcctcctgg ccactgacgg
    12001 ggacaaactc gcgcttacgc cgacgtacta cgtctatgac cttctccaac ctcacaaagg
    12061 agcgacggca atcgtgaccg aggttgacgg accttctgtg gaggttaccg acgccggacg
    12121 gaggagtacg ctcccggttc ttgatgcatc ggcaacagca tcccctgacg gcaccatgac
    12181 gatttctgta atcaaccggc acccttcgga ggctcaggag ggatgcgtca aagtggccgg
    12241 aagtacttct ttccccaaca tctccgcccg ttgcctcggg ggaacctcgc ccggcgccga
    12301 gaacaaaccg ggggcaaccg aatcagttgc tccggcggag gtcagcacgg ccccggtgaa
    12361 aaacggggaa ttcgtgcaca cgttcccgcc ctgttccgtg acggtgttcc gtctcagcca
    12421 cgtgtaaccc gccgccggtg tgagttccgg ccgcgcccta tcccgcgcgg ccggtttgcc
    12481 accgttcggt gtctcctcct accttcgaca ggacgatgac aagaccgaac cattcacctg
    12541 gagacacttt atgacgcttc ctgacgcctc ttgtgagccg atcgtgcttg cgtattacta
    12601 tatctggttc accagaggct ggtttgatgg atcccacacc ccgcaataca aagccacaat
    12661 ggcggacatt catcccgcca tcgggtcgta cgattcccat gatccggacg tgatttcgga
    12721 gcacatgaag cagtgcgtga aggcgaaact tgatgcgctt gcggtaagct ggttttacca
    12781 gaaaaggaac cttaccggcg cgacgagcga tgacatactc gaatcagtaa tgacagctgc
    12841 ctcagatcac ggacttcgaa catgtattga catggaggcg gccgggcttt gcgccgaccg
    12901 gttgtatgag gccttgaagc actacctcag tcgttattcc aatgacccgc gcgcacttca
    12961 cgcgcagggt aagcctgtgg tgctgtgctg ggccacatgg aaactcgggg cggatgtgtg
    13021 gactcagatc acgaaaaggc tgcgagaaca gggcatggac gcgttccacg tcgcctccaa
    13081 ccagatggat ccttcctact tctcgttcct cgatgcgctg gagtgttaca cgccgctgtc
    13141 gccggagcag accgtcatgg agcagtacac cggcgcacgc gccgccgtag atgcgtataa
    13201 cgaaacgcct gccggccgga ccagaccggg aagatggcat gccacggtga tgccgggttt
    13261 cgaggatcgg ttgatgaccg atcggctcaa acgggacgac tacgcgcatt tccgtatgcg
    13321 gcggtcaggg tactattact gggaaacttt tgccgccgcc atggcgtctc gcccagaatg
    13381 gcttcacgtg acgagtttca acgaacttgc cgagcattca cacatagagg aaacggttga
    13441 ggatggagat ctttacctcc gtcttactgc ggaatttgcc acccatttca aagccgcgcg
    13501 aaccgcctgg aagtgacaga accgaagcag gttcaacccc gctcttgtgg agaaatcgat
    13561 gccaagaggt caaaatctgg gaaccgaacg catagatcgc cgcgacgcgg acaccggtct
    13621 cctggtgcgc cagatcacca gcttccccac cccgagcact catctccact acgaaacgcc
    13681 aacgttcact cccgacggac ggcggatgat cttcctctcg atgcgcgttc cggaacgtgg
    13741 cgcgcccagt gatctcttta cggcaggaag cgatgggaag gatatcgttc aactgtcaga
    13801 tgaccaccct tcgggggtat cgaatccgac tctggcgaag gacgggcagt cggcttatta
    13861 cacacacggg ggaaccgcct accgtacccg cctcgaaaac ctggagaccg tggaactggg
    13921 ccatgtaccc ggcgcctccc agcatgcgta cccgcgggca atgtgttcgt gggatggccg
    13981 atggtacttt gcgatggcca ggttggcgaa cggcaggatc ggccttgttc gctgggacgc
    14041 aggcactggt gagcacgttg tcgtgctcga agcggacgga atcaaccatc cccgtgcaaa
    14101 cccgggaggt ccagagatcg attgcagtgt gaagacgcga cgcccggatg gctccattga
    14161 gtcgcgaaag gttcgtttcc actgcgagac gctcaaggaa atgaacgctg atttccccac
    14221 cggcgaattc aagaccgccc actcatgctg gctcggggcc acgggtgatt atcaaggcac
    14281 gctccagatg cctcacaggg gcgtgatcgt catgagaaga ggggcggcgg agcctgagaa
    14341 agtggcattt ggcgggccat atttctggca ttccggcgct tccaatgatg gcaaatggat
    14401 cattgccgac acaaactggc cggacgaagg cctctggctg ttcaacgtgg gaaccaagaa
    14461 acgagaacgg atctgtttcg cgggggcatc acagggtcac ccgcagagca cgcacacaca
    14521 cccgaatctc tcaggcgatg gcgcgatggc ggtgttcacc tccgatcgca ccgggatgcc
    14581 tcaggtgtat gtggcgtaca taccggagga aatgagggat cgcctggcgc gagccgattg
    14641 aaaaccgggt gaatggggcg gtctgcggac cttgaactcg acgcgttgcc cagcttcctg
    14701 tccaacggga ggtgtgcggg gcggattctt ttacggagcc gattccctta gtcgggttcg
    14761 ggatacgcgg ttactgctga aactccggta gttcgacggc acacgctcct tcgaccggcg
    14821 ccatcacttc gatgaacggg tcgcgctcgt gtttttcgaa gtactgcgta atcaacgcat
    14881 tccgcagatt ttcaacggca ccatcatcta cgattgcttc cacgcaccca ccgaggccgg
    14941 caccaatgag accggcaccg tacaccccat ccactgaacg agcaatgtcc acgatttcgt
    15001 cgagttcggc gcaactcgcc gcgtaaccgc ccggttgaag cgcgagccgg gcagcctccc
    15061 gaatctcggg gtccggcgac tgagacgcct cgatgagacc atccaggtcg gcgtcgctaa
    15121 cccctgaatc gtacggaacc ctgacgccgg aatcatcaag aacggttaca cggtcgcctt
    15181 cgtgtgagag gtccatgagc cttccggcac cggcaatgtc gcccctgcgg agaaaatccg
    15241 cgagaatcag gctgcgttcg cactcggcca gcccgtacag gcagacgtcg cgaacccggt
    15301 aaccttctgc aggatcatcg tgcgtgctga agagcttttc cagttcttcg cgtgcgcccg
    15361 gaagagccga atgtatctgt tcacgtgtgg cacgaaccgg caattgcttg aggacgcggt
    15421 aaatttccga cacgtgaaca ccgaggcgaa gcacgtcacg gagatactcg agccggcctt
    15481 gccactcagg ccgagctttc ttaatccaca gcagcgcaat tacatatgtg gcaacgcgtt
    15541 cgttgaaaac gctgcgtgcg ttcgccgttt tctgtgcatg gacgcgggaa tgacacacca
    15601 caaccgacac gccgggtcgc cagggactcc agtccacgtt catagggaaa aagtccacat
    15661 gcgacacgtg ccctgaacga ctcaacgcga ttgcggcctg gtcgccaaac cctccgcgcg
    15721 tgcccacata ccattcggcc tggccgcacc cctctgcaag cgcccgtcgg tcaatcggct
    15781 tctcgttcgc caggtgaagc gcgtaccccg cagccaccac aagcgcggac gatgaactga
    15841 gacccgccgc ctgtggcaac tggctgtcac ccagaatatc caggcctcga atcgaatcgc
    15901 gcatacccca tgaattcgca aaatacgccg ctgccgatct cacatacgag ctccaatgac
    15961 cgagaagccc cttctgccgg agcgtttccg gcgtggtgag cgtccagtcc gcccaatccg
    16021 tgatcttatt cccaggaagg agctccggca ccgcgaactc gtccgcggga aacgacgggt
    16081 tcacgttggc tatccggatg cggttatcag acctgggaac gcagacaagc accagttccc
    16141 tgaccgcgat cggattgacc cgaccacctc gatggtctat gtgcatgccg aggagattca
    16201 cccgccccgg agcgcgaacg atgaacatcg ggacgtcatc attgtggccg aacaccgact
    16261 gcgcacgttc cacgagtgtg gcgtaccaac ggatcgcgct tcccgcctcg gacggagact
    16321 ggttgcgcat gcgtgcgacg cggttgatca gcgcggggga gggcgcagta agggcagcgc
    16381 gccaggaacg gacagcaggg aagttcgcca tctggcgctt ccttcagaaa gaagtgagca
    16441 ggggcagcct tcgcggccgc ccctgacgaa aacatcggct cggattgcta tttcttcggc
    16501 gctaccacgc ccttcacgag aatgagattc gcgtagaggg ctgtttcgcc cgtcatcacg
    16561 atcgcgaagg ctttctcttt cgtgaagtca tagaatgcga accggtccag ctcgacaagt
    16621 ttcacatcac gtttttcggc tttgtt
//
LOCUS       DAOSOO010000053        16497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3544_length_16497_cov_5.294186, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000053 DAOSOO010000000
VERSION     DAOSOO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3544_length_16497_cov_5.294186"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLT86_05625"
     CDS             <1..1065
                     /locus_tag="PLT86_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36124.1"
                     /translation="PICGEVLRAKLVERVDADSDGESLALLMAGRLNRLLCPVCGHGG
                     VAPEPVLVHSDAHRTCAAFVPFTIPANRKSHLAGKFKGLAQRVAGQYGHAHVCFGLKQ
                     LREALPWLPAFPNSARGFQRFDAPGSAFHQVHALRRALKNRGGDVRLLARLGTALYAS
                     GDLKGARKTMETVVREEPDNGDAWRCLGAILLDSGQARPAAEAFDRAYRHHRDPECLF
                     LAGVAAFRAGAPLEALTRFQRVIQKRADFFHAYIWIAVVHTAEGRYGPAIEALRAAVM
                     NGLKDAGMIRSRKELSPLFGERGFQSLMKHLEKPQARGLNRPSATKRGTGSGRPGAKR
                     SGRRTPTSPVQKRRQAVKGK"
     gene            1079..2104
                     /locus_tag="PLT86_05630"
     CDS             1079..2104
                     /locus_tag="PLT86_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOT36125.1"
                     /translation="MRRASVLFALLALFCGAGQASPPIRVAVTLTDFLPIVEAIGGSQ
                     VEAFSIMPPGSDPHSVSLNTEIIQRVREAQIAVYAHSASLAFEHDLKNSLAGKPSVDW
                     PQYEQQGARLLDFPGMPSNPHGFWLDYDNAVAIARAVASELTKMSVDEKVVAANLKRF
                     SDEVHAQREAALQAMRDCGSLGDTVVAVVPGVAYTVRNLGYAVGGILLPDEGSGQADA
                     KSLMQINRLLGKGGARLLVCPVGLRNAKAGEIAEQLAKDTGTRVAYTRFLQTGGTNRT
                     FLGDAAYNAMVTSTVLVDVQPEAGGLANKSIYPTLLAIMGGVFLLLIFLVIRRILSRQ
                     ELHGRRS"
     gene            2088..2894
                     /locus_tag="PLT86_05635"
     CDS             2088..2894
                     /locus_tag="PLT86_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOT36126.1"
                     /translation="MADVADDRNPAVEVRELSYSYQRPLVEALRGVSLTLPRGTFTAV
                     MGPNGSGKSTFLRLLLGILRPTRGEIRVLGESPTAPSQRVQRLIAYVPQYDSINPLLP
                     LRSKEVIALGLSSRVTDRPARWIDERVSQILEDLDLVEIRDHQYGLLSGGQKQRVLLA
                     RALALEPELLVLDEPFSAMDMTSQHATAKLLSGLVRQKGKTVITVVHGIHTIVHHLDN
                     LVLLNREIVAAGTPDEVLRPANLTRAYGTDVPVVICPEGFRHPLVEDVHA"
     gene            2887..>3113
                     /locus_tag="PLT86_05640"
     CDS             2887..>3113
                     /locus_tag="PLT86_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOT36127.1"
                     /translation="MPDSVSYLTSPFVLRAAAAFFFVAINAALSGVFANFRNSTFLVS
                     GASHAALGGAALVIVLESRGSAFGISPVFGAA"
     assembly_gap    3114..3213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3214..3567
                     /locus_tag="PLT86_05645"
     CDS             <3214..3567
                     /locus_tag="PLT86_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOT36128.1"
                     /translation="QFLFVSFDLDGARAFGINADRFNYLLFALIGLSSALLVKAIGSI
                     LVFAMLSAPAATALLISDSVKKTLWWSFAIAFVAGILALVLSYFTALSVSAIAAFLVT
                     TAYFAVRIWRFASAQ"
     gene            3586..4989
                     /locus_tag="PLT86_05650"
     CDS             3586..4989
                     /locus_tag="PLT86_05650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT36129.1"
                     /translation="MPKITFSGATRTVTGSMHLLETGGKRILLDCGLFQGKREEAFKR
                     NRHFPFDPASITDVVLSHAHIDHSGNLPTLVRKGFTGRIHATHATRDLCAVMLLDSAH
                     IQEKDVEFVNRKRARKGEEPFNPLYTMVDAHRTIDRFVSYGYHQPFAIGGTSIEFFDA
                     GHILGSASVKLTFKGRGTPSTLAFSGDVGRKNLPILRDPEPFDHADWLICESTYGARL
                     HHPPSEVENRVAEVVNTTYRQRGKIIVPAFSVGRTQEIVYALNRLANANRIPRLPIYV
                     DSPLSTNTTEVFRLHPECYDEETRQFLEEEGDPFGFSQLQYTRTVQESMALNSRTDPM
                     IIISASGMCEAGRILHHLRNNIEDNRNTIMIVGFQAENTLGRKLVDGHREVKIFGEEH
                     TVRANVVVIEAFSAHADWKEMQEWYAPVGSSVKRTFLVHGDDEALAGMKEHLQPLTGG
                     AITVCENGQSFDLSGKT"
     gene            4986..5696
                     /locus_tag="PLT86_05655"
     CDS             4986..5696
                     /locus_tag="PLT86_05655"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HOT36130.1"
                     /translation="MIRQDYLMRLLDQLAQALARVLSLRENEARGQALDFLNRESERL
                     IGIDGAMLEILSPDAIRKATASVEKGFVAARILEEISLISEEEGDHHRATAAAVKAFW
                     LYAAAAQADASAADNTFRERIAGFGDACAERGLAEGDLLSLMAGYEMLGEFGRAEDAL
                     FALVEVARRPGDLLPVGRDFYARMAEHEDAELEKGGLPRNELQDGIDGFLARVQSRLS
                     QIEIPDEAREAERAANSS"
     gene            5771..6295
                     /locus_tag="PLT86_05660"
     CDS             5771..6295
                     /locus_tag="PLT86_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36131.1"
                     /translation="MKKLLVAMLLTLSMVSFAAAEGSAAGSKAAGLGIDLAVPFGDLS
                     NVAGVGYGLTGQFDYGFNQKITLIGSIGYMMFAEKNNWEGSSIPIKVGATYCLANVMP
                     GLYAIGEVGFDMLSFDWSGPFGGSSSHNELCLVPGIGWQKPMGSMTLDLSARMSLVDG
                     GSRIGIRAGVKKAL"
     gene            6444..7766
                     /locus_tag="PLT86_05665"
     CDS             6444..7766
                     /locus_tag="PLT86_05665"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36132.1"
                     /translation="MNERVVLIGAGSVSFTRGLVADLLDAWPKGELALVDIDPAALEV
                     ATNLARKMIAARGAGWKLEASEDRRTVLKGATAVICTVGVGGRRAWEQDVFVPRKYGI
                     FQPVGDSVGPGGSSRALRMIPAMVDIAEDILDLAPDALFFNYGNPMAPVCRAIRKATG
                     APVIGLCHGVNHTAHYLAHALGVSAARCTYNAVGMNHLTWFTEFRVDGADAMPRLREI
                     AAQRVSEAKGNPAAVAELSPFSWQLFLLFGAFPAVLDRHVVEFFPQFFRDGNYYGLTL
                     GVDAFSFEETIAAGDREFAAMQKDASSPDPLPGQYHARGNGEHEQVVSIISSMREDSG
                     SVYSANLPNMGQVADLPAGAIIESPAIATTRTLLPVVQPRLNPGIPGTLATRLLWVET
                     IVDAALERKRDLFIQALVLDGFAASLDDAAAMADEFLRVHAPYLGDFD"
     gene            7832..9862
                     /locus_tag="PLT86_05670"
     CDS             7832..9862
                     /locus_tag="PLT86_05670"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT36133.1"
                     /translation="MGSFRRVESLATPQELQTAKSWFDARVSESSAEVPFAFSYGGQP
                     WTSMMRQWDHAEDRSSSDAGKTRRSIRWRDRGTGLEVRCEALLYEEFPVVEWTLYLKN
                     TGVTETPIISDLMPLDTCVARGDAGSTDLSEFLLYHHAGSTCTPGDYRPFESVLAPAT
                     TKTIATSGGRSSDENFPYFNLTYPIRNGAAGMVIAIGWPGQWKAQFLRDDGIGLHISM
                     GQELTHFRLHPGEEVRTPLIAILFWLSSDSGAGVGRDRLADRWRSQNLWRRWMLAYNV
                     PTEADRQPLKPRLMACSSHQYGEMVEADEASQIMFIDRYLEEGLNPDYWWMDAGWYPN
                     EWGWPNTGTWEVDTKRFPRGLRAITDHAHSRGLQTLVWFEPERVTPGTWLYTHHPEWL
                     LGKDGEQKLLDLGNPAAREWLTDHVDRILTEEGIDLYRQDFNMEPLPYWRANDTPERA
                     GISEMRHVDGLLEFWDELRRRRPGLLIDTCASGGRRNDLETLRRAVPLWRSDYILEPV
                     GQQSQTWGIALWIPYFGTGTNHEDAYGFRSVMVPNVNALWDVRRRDLDYDLIRNLVAQ
                     WRGVGELFGGDYYPLTPYSLENDAWIGWQFHRPDLEKGVLMLFRRPESPRASDRFPLW
                     GLDVEGRYRIVDLDVRERVSVLGGDELTATGVSVDIVAAPGSAMLVYERVAS"
     gene            9881..12088
                     /locus_tag="PLT86_05675"
     CDS             9881..12088
                     /locus_tag="PLT86_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36134.1"
                     /translation="MTTLPRDPHEGFTLENDVLGVRLWGPPASPALLLGASDIWDRRW
                     FAEQQPLVTLEKIRELAFADRLAEIARSPNETIYHLYQRYDFPCPKPGAQIVFLTPFG
                     TDAHAEHTGNSLQLRVTGPRKTLIVEIWVSLTRRLVVCECTQQGLEPTDLAVRVYRHR
                     DTILPGEPTDPTIGGHPSAMDFAPFPMPDAFCLPIGNGGGVKQTFPPDSTFEAGFVFA
                     AAASVVGLRASLHLDTSGRGLGAPYWAEKEGRLDHGTVKRYAPINSAEGAAATLALHE
                     PGDRFCILATVATSQDSLDPIQEAANTLVEAERQGVAFLRAEQKKALDSARRARRAIA
                     TLNGQTVLNAPATILPNLRRKGGYYGDIPMCSVGPTKFCFQDAGLWHADFHLNEIRAE
                     PLLTLGRFEDVRLFADMIRDLLPQAEENAADVYGLPGAMYPLVHFPLRCRGIAHTNLT
                     WEQDIGLNGLVAKPLWLFFRYTGDTAFLRDTAYPVMRSCADFCTAYLSEGEDGHLHIV
                     PTVSPEHWGLTARFERNRDSTSALSLTKYLLNATAEAADLLHTDSQQAERWRAAAARL
                     APYPTCVVDGEEIWTDVAGAPPIEYNIPVPLSPVFWGDDVGLDSHPEAKALAYRTLRH
                     IRVWEPHRFYLDSCVRPRLGVPSPNAPIVPESMLLSYQSLHIFPGVPPDVPVAMQNFA
                     AEGGFRVSATKTETGRIEEIIVYSACGGVCRLTDPRTGALLQYEMQPDETLRL"
     gene            12196..13173
                     /locus_tag="PLT86_05680"
     CDS             12196..13173
                     /locus_tag="PLT86_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOT36135.1"
                     /translation="MQKTESSALMSLLEVLRRNWPLKVASFIVGFTLWLHVKTDQTYE
                     RVVSLPLEIREPSGRFVVSNDVPRTVQVRISGSGKQILVGLRHARVVLKPRVNRPEAL
                     TLDISTDDVQGLDPNRGIVVTNIEEPRSVLLDFDFLEMREAQVIPRVGIGVKPGYTVV
                     GDVRVDPPKVRLGGPRNVIGSIRTVLTDSVVLSDVSSDVGELVTLELPREMGLVASPE
                     TVRVRATVQALAERRFSDIPVVVTNLPKRVNLIANPAVVAVDLTGGEHVLENVSADQI
                     SAEVDYQQSAQGGGADLPVKVSVPPGVRVKQITPEIVRLVPGQRSESVR"
     gene            13170..14186
                     /gene="tsaD"
                     /locus_tag="PLT86_05685"
     CDS             13170..14186
                     /gene="tsaD"
                     /locus_tag="PLT86_05685"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT36136.1"
                     /translation="MIVLGIESSCDETAAAVFDTESGLRSNVIASQAEHIPFGGVVPE
                     MASRAHTERIVPVVRQALADAGVTLDEIHALAVTAGPGLIGSLLVGVSFAKGVAFTRN
                     LPLVPVHHIEAHVFASRIEHPDLAPPFLALVVSGGHTQLIWVPKWGTYRVLGTTRDDA
                     VGEAFDKVAKILGIGYPGGPLIDRLARDGNADGVKFPKAFIKGDPFAFSFSGLKTAVL
                     LAVEKTPENELELRKADLAASFQNAAVGALVQTTLRAARKMRTKRVVLAGGVASNSAL
                     RREMEAACARIGIEVTRPSPVFCTDNAAMTACAGAFWFERGRRAGYNFNPNPRLPLDA
                     FGNE"
     gene            14224..>15304
                     /gene="alr"
                     /locus_tag="PLT86_05690"
     CDS             14224..>15304
                     /gene="alr"
                     /locus_tag="PLT86_05690"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT36137.1"
                     /translation="MAGATKATVDLEALRANVRFVRELVGKGRLILACVKANAYGHGA
                     LRCANAALQAGADALGVARLDEALEFRQNGIGGRIVLIGPESLEATNDLVRADVEIAI
                     DCADRLEAVLRAGKASRRVPIVHLAVDSGMARFGASFEEASQLAVRLSQAPEVSWAGV
                     MTHFPVADTDQQRSVEQWRRFHALLSGWKTRGIAVPCCHAANSAAIMGLPETHADMVR
                     PGIMLYGIPPGTAHTSDKLRPVLRLETSVVALHWREEGDYVGYGETFRCNRRTLIATI
                     PAGYGDGIPWTAGNRSWVLVNGRKAPVVGRISMDQTTIDVTDLREVHLGDPVVLIGSK
                     GDQAITANDWARWCGTISYEITTRLR"
     assembly_gap    15305..15404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15405..16114
                     /locus_tag="PLT86_05695"
     CDS             <15405..16114
                     /locus_tag="PLT86_05695"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT36138.1"
                     /translation="LETGLLTEIDTVSDLSQIAPRVAELLVGQRYAVIGAVVSWAAAR
                     VASLLAGNGTGVTGMTSLSGFGLRIDYEHGEPGVDRVAACAEAYARKGGPLVLVGAGT
                     ALHTNVVTREGVYLGGAIMPGFRLMFEALTAGTDQIRVVSPSVPSRVIGRSTPECVQI
                     GVYHSWLGGAVRLVRRTCEEVGGHPALWLTGGQAEWLKTEFPEAEFDPDLTLKGLARA
                     FCGADQQNYRNSQNRTA"
     gene            16168..>16497
                     /locus_tag="PLT86_05700"
     CDS             16168..>16497
                     /locus_tag="PLT86_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36139.1"
                     /translation="MEYRGKYPYFPYEKISTYPIKNRKNKVRQEDLYFPDRVMAMTPE
                     FSHPELGNIADTIVKARDKGYPVIWLMGAHPVKAGMSPLIIDLMQRGICTLLCGNFAS
                     AIHDFELA"
BASE COUNT         3305 a         4684 c         5048 g         3260 t
ORIGIN      
        1 cccatctgcg gcgaggttct ccgggcgaaa ctggtggaac gcgtcgacgc agactccgat
       61 ggggagagcc tggcgctcct catggcgggg cggttgaacc ggcttttgtg ccccgtgtgc
      121 gggcacggcg gcgtggcccc ggagcctgtg ctggttcatt cagacgccca caggacatgt
      181 gcggcgttcg tcccgttcac gatccctgcc aacagaaaat cgcaccttgc aggcaaattc
      241 aaagggcttg cccagcgcgt tgcggggcaa tatggccacg cgcacgtatg tttcgggctc
      301 aaacagctcc gggaagctct gccctggtta ccggcattcc cgaactccgc gaggggtttc
      361 cagcgttttg atgcaccggg ttcggcgttt caccaggtgc atgcgctccg ccgtgcgctc
      421 aagaatcggg gaggtgacgt ccggttgctg gctcgcctgg gaacggcgct gtacgcttcc
      481 ggggatttga aaggagcgcg caaaacgatg gaaaccgttg tccgcgaaga gcccgacaac
      541 ggagatgcgt ggaggtgtct gggtgcgata cttctggatt ctggacaggc gaggcccgca
      601 gcggaggcat tcgaccgcgc atacaggcat catcgcgacc ctgaatgcct tttccttgcc
      661 ggagtggccg ctttcagggc aggggcgcct ctggaggcgt tgacgcggtt tcagcgggtg
      721 attcagaaga gggccgattt cttccacgcg tacatctgga ttgcagtggt acacaccgcg
      781 gaagggagat atggaccggc catcgaagcg cttcgcgcgg cggtgatgaa cgggctcaaa
      841 gacgccggga tgatccgttc acgcaaggaa ttatccccgc ttttcggaga gcgcggattc
      901 cagtctctga tgaaacacct ggaaaaaccg caggcgcggg gcctgaatcg tccgtccgcc
      961 acgaagcgag gaacgggcag cggccgaccc ggcgcgaaac gttcgggccg aaggacgccg
     1021 acgagtcccg ttcagaagcg caggcaggcg gttaagggga agtgaggagg acagaactgt
     1081 gagacgtgcg tccgtgctgt tcgccctgct cgccttattc tgcggagccg gacaggccag
     1141 cccgcccatc cgcgtggcgg tgacactgac ggatttcctt cccatcgtgg aggcgatcgg
     1201 gggcagtcag gtggaagcgt tcagcatcat gccaccgggg agtgaccctc acagcgtatc
     1261 gctcaatacg gagattattc agcgcgtccg cgaagcgcaa attgccgttt acgcacacag
     1321 cgcatcactc gccttcgaac atgatttgaa gaactcgttg gcaggaaagc cgtccgttga
     1381 ttggccgcaa tatgaacaac agggggcccg gttgctggat ttccctggaa tgccctcgaa
     1441 cccccatgga ttctggctgg attacgacaa cgctgtggca atcgctcgag ccgtcgcgtc
     1501 ggaactcaca aagatgagtg tcgacgagaa ggttgtggcg gccaatctga aacgcttctc
     1561 ggatgaagtg cacgctcagc gtgaagcggc cctgcaggca atgagggact gtggcagcct
     1621 cggcgacaca gttgtggcag tagttccggg cgttgcttac accgtgcgga acctcggcta
     1681 tgccgttggg ggtatcctgc ttcctgacga aggaagcggc caggcggatg ccaaaagcct
     1741 catgcaaatc aaccgattgc tcggaaaagg tggcgcacgg ttgctggtgt gcccggtagg
     1801 acttcgaaac gccaaagcgg gggaaatcgc tgaacagctt gctaaagaca cggggacccg
     1861 tgtcgcgtac acgcgttttc tccagacagg tggaacaaac aggacgtttc tcggggatgc
     1921 agcgtacaac gcgatggtaa ccagcaccgt tctggttgac gtccagccag aggctggtgg
     1981 actcgcgaat aagagcatat atcccaccct gctggcgatc atgggcgggg tctttctcct
     2041 gctcatattc ctcgtcatac gtcgcatatt gtctcggcag gaattgcatg gccgacgtag
     2101 ctgacgacag aaatccggcg gtcgaggtcc gtgagctgtc gtactcgtac cagcgtcctc
     2161 ttgtggaagc gctgcgcggg gtcagcctga cgctgccaag gggaacgttc accgccgtca
     2221 tggggccgaa tggatccgga aagagcactt tcctccgtct cctgctcggc atactgcggc
     2281 ccacgagggg tgagatccgc gtactcggag agagcccgac ggctccgtcg caacgcgtgc
     2341 aacgtctcat cgcgtacgtt ccgcagtacg attcgatcaa ccctctcctt cctctgcgaa
     2401 gcaaggaggt catcgcgctg ggactctcgt cccgagtgac tgatcggcct gctcgctgga
     2461 ttgacgagcg cgtttcccag atactcgaag accttgacct tgtggagatt cgcgaccatc
     2521 aatacggcct tctctccggc ggccaaaaac agcgggtgct gcttgcccgg gcgcttgctc
     2581 tcgaaccgga gctactggtc ctcgacgaac cgttttccgc gatggacatg accagccagc
     2641 acgcgacggc aaagcttctt tcaggactcg tacgccagaa agggaagacg gtaataactg
     2701 tcgtacacgg gattcacacc attgtgcacc acctcgacaa cctcgtcctc ttgaacagag
     2761 agatcgtggc tgccgggacg ccggacgagg tgctccgccc cgcaaatctt acgcgggcat
     2821 acggcactga cgtgccggta gtcatctgcc cggaagggtt ccggcacccg ctggtggaag
     2881 acgtccatgc ctgattccgt ctcctatctc acgtcaccgt tcgttctccg ggctgcggcg
     2941 gcatttttct ttgtggcgat taatgccgca ttgagcggtg tatttgcaaa tttccgcaac
     3001 tccacctttc tggtttccgg tgccagccat gccgcactcg ggggcgcggc gcttgtgatc
     3061 gtactggaaa gccgcggcag cgcttttggt atcagtcccg tcttcggcgc ggcnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaattcc ttttcgtctc tttcgatctc
     3241 gacggcgccc gggcatttgg catcaacgca gaccgattca actacctgct ttttgcgttg
     3301 atcggccttt cctcggcgct gctggtaaaa gcaatcggat cgatactcgt tttcgcaatg
     3361 ctgagtgctc ccgccgcaac ggcgttactt atttctgatt cggtcaagaa gacgctctgg
     3421 tggtcctttg cgatcgcctt tgtggcaggt attctggctc tggtgttgtc gtatttcacc
     3481 gctctttcgg tgagcgccat cgccgcattt ctcgttacaa cggcctactt tgcggtacga
     3541 atttggcgat tcgcgtccgc gcaatagaac gaacaaggag gttttatgcc caaaatcacg
     3601 ttctccggag ccactcgaac agtgacgggg tccatgcacc ttctggaaac cggcggcaag
     3661 cggatcctgc tcgattgcgg gttgttccag ggcaaacgcg aagaggcatt caagcgaaat
     3721 cgacatttcc cgttcgatcc ggcctcaatc accgacgtcg ttctttccca tgcgcatatt
     3781 gaccattccg ggaatcttcc gacgcttgtt cgcaaaggct tcaccggaag gatacatgcg
     3841 actcatgcga cgagggatct ttgcgcggta atgttgctcg acagcgcgca catccaggag
     3901 aaggacgtcg aattcgtcaa ccgcaagcgc gcgcggaagg gcgaagaacc tttcaaccct
     3961 ctttacacga tggttgacgc ccaccgcaca attgaccggt tcgtcagcta cggatatcac
     4021 cagccgttcg ccatcggggg cacaagtatc gaatttttcg acgcagggca tatccttggg
     4081 tcggcctccg tcaaactcac gttcaaaggg cgaggcacgc cttcaactct cgcgttctcc
     4141 ggcgatgtcg gacggaagaa tctccccatc ctccgggacc cagagccgtt tgaccacgcg
     4201 gactggctaa tttgcgaaag cacgtacggt gcgcgattgc atcatccgcc ttctgaggtc
     4261 gaaaaccggg tggcggaggt tgtgaatacc acgtatcgcc agaggggaaa gattatcgtt
     4321 cccgccttca gcgtgggaag aacgcaggag atcgtctacg ccctgaaccg gcttgccaac
     4381 gccaatcgaa tccctcgtct cccgatttac gtggacagtc cgctttcgac gaataccacg
     4441 gaagtctttc gcctgcaccc cgaatgctac gacgaagaaa cacgccagtt tctggaagaa
     4501 gaaggtgatc cgttcgggtt ttcgcagctt cagtacacca gaaccgttca ggaaagcatg
     4561 gcgctgaatt cgcgtaccga cccgatgatc atcatatctg cgtccggaat gtgcgaagcg
     4621 ggtcgcatcc tgcaccacct tcgcaacaac attgaagaca acagaaacac catcatgatt
     4681 gttggattcc aagcggagaa taccctcgga aggaagctcg tggatggtca tcgcgaggtg
     4741 aagattttcg gagaggagca caccgtccgc gccaatgttg tagtcattga agcattcagc
     4801 gctcacgccg actggaaaga gatgcaggaa tggtatgcgc cggtaggctc atccgtgaaa
     4861 aggacgtttc tcgtgcacgg ggatgacgaa gcgctcgccg gcatgaagga gcacctgcag
     4921 ccgctaaccg gaggcgcaat caccgtgtgt gagaatggcc agtcattcga tctcagcggg
     4981 aagacatgat ccggcaggat tacctcatgc gcctgctgga ccagttggcc caggcgctgg
     5041 cacgcgtgct ctcgctccgc gagaacgagg cacgcggtca ggcactcgat ttcctgaacc
     5101 gcgagtcgga gcgactgatc ggaattgatg gtgcgatgct ggaaatcctt tctccggacg
     5161 caatccggaa ggcgactgct tccgtggaaa agggcttcgt tgcggcgcgc atcctcgagg
     5221 aaatctcgct catttctgaa gaagagggcg accaccaccg ggcgacagcc gcggcggtga
     5281 aagcattctg gctgtatgcc gcggccgctc aggccgacgc gtcggcggcc gataacacct
     5341 tccgggagcg aatcgcaggg ttcggggatg cctgcgcgga gcggggtctg gccgaaggcg
     5401 acctcctttc cctgatggcg ggatacgaaa tgctcgggga gttcggcagg gcggaggatg
     5461 ccctgtttgc gctggttgaa gtggccagaa gacccggaga ccttctcccc gtggggaggg
     5521 atttctatgc ccgtatggcc gaacacgaag acgcggaact tgaaaagggc ggccttccga
     5581 gaaacgagtt gcaggacggg attgacggtt ttctcgcgcg cgtccaaagc cggctgagtc
     5641 aaatagagat accggatgag gcgcgggaag cggaaagggc agcgaattct tcttgacagg
     5701 cagcagaacc agtgctaact ttttttgcgc aggggggtaa ggtaacagca cacattacag
     5761 gaggttggtc atgaaaaagc ttctcgtggc gatgcttctc acgctttcaa tggtgtcgtt
     5821 tgcagcggct gaaggctctg cggcaggttc caaagccgcc gggctgggta ttgacctcgc
     5881 tgtcccgttc ggtgatctga gcaacgtcgc cggagtcggg tacggcctca ccggtcagtt
     5941 cgattacggg ttcaaccaga agatcacgct catcggctcg atcggctaca tgatgtttgc
     6001 agaaaagaac aactgggaag ggtcgagtat tccgatcaag gttggagcca cgtattgcct
     6061 tgccaacgtg atgccggggc tctacgcaat tggtgaggta ggcttcgaca tgttgagctt
     6121 tgactggtcc gggccgtttg gcggctcgag ttcccacaac gaactatgcc tggttcccgg
     6181 tatcggctgg cagaagccca tgggcagcat gacactcgac ctctccgctc gcatgagcct
     6241 cgtagacggt ggcagccgca tcggcatccg cgcgggagtc aagaaagccc tctgactctc
     6301 gactcctggt taacggagag cgggaccccg gaaaaggtcc cgcttctttt tttgcccttg
     6361 ccccctgagg cccactgtca gacgtttttc ttggttcacg ctcttttctt tcctttcacc
     6421 gggaaaaatg gagtcctttg aagatgaacg aacgagttgt tctgatcggc gcgggaagcg
     6481 tctcttttac gcgcggcctt gtagccgatc tgctcgacgc atggccgaag ggcgagctgg
     6541 cactcgtgga cattgacccc gcagcgctgg aagtcgccac gaatctcgcg cgcaaaatga
     6601 tcgcagccag aggtgcgggc tggaagctcg aagcttctga ggatcgcagg acggtgctga
     6661 agggcgccac tgcggtaatc tgcacagtgg gcgtgggagg acgacgcgcg tgggaacagg
     6721 acgtgtttgt ccctcggaaa tacggcattt tccaaccggt gggggattcg gtgggaccgg
     6781 gagggtccag ccgcgcgctg cggatgattc cggcaatggt ggacattgcg gaagacatcc
     6841 tggacctcgc acccgacgcg ctgttcttca attacgggaa ccccatggca ccggtatgcc
     6901 gggcaatcag gaaggctacc ggcgccccgg taatcgggct gtgccacgga gtgaaccata
     6961 ctgcgcatta ccttgcacac gcgctcggcg taagtgccgc acgttgcacg tacaacgccg
     7021 tcggcatgaa ccatctgacg tggttcacgg aattccgggt tgacggtgca gatgcgatgc
     7081 cccggttgcg ggaaatcgcc gctcaacgcg tttccgaagc caaaggaaat cccgccgcag
     7141 ttgccgagct gtcgccgttc agctggcaat tgttcctttt gttcggcgcc tttcccgcag
     7201 ttcttgaccg gcacgtggtg gagttcttcc cccagttctt ccgcgatggc aactattacg
     7261 ggctcacact gggtgtagac gcattcagct tcgaggagac gattgcggcc ggcgaccgcg
     7321 aattcgccgc gatgcagaag gacgcatcat cgcccgatcc gctccccgga cagtaccatg
     7381 cgcggggcaa cggggaacac gagcaggtcg tgagcatcat ttcgagcatg cgagaggact
     7441 ccggatcggt gtactccgcc aacctgccga acatgggcca ggttgccgat cttcccgcgg
     7501 gcgctatcat cgaaagcccg gcaatcgcaa ccacgcgaac ccttctcccc gtcgtacagc
     7561 cacgtttgaa cccagggata ccgggcacgc tcgcgacgcg tctcctgtgg gtggaaacga
     7621 tcgtagacgc cgcgctggaa agaaaacgcg atctgttcat acaggcactg gttctggatg
     7681 gatttgccgc ctcgctggat gatgcggcgg caatggcgga cgaattcctt cgggttcacg
     7741 ccccctatct gggtgatttt gactgaccaa ccgcgggagt ttggtgcgat catcgagcct
     7801 cgtatcgggc atctttttgg agggagatcg aatgggttcg ttccgtcgtg tggagtctct
     7861 ggccactcca caggagctgc agacagcgaa gtcgtggttt gacgccagag tcagtgaatc
     7921 ctccgcggaa gtgcccttcg cattctccta cggaggacag ccctggacct ctatgatgcg
     7981 ccagtgggac cacgcggagg accggagttc ttccgacgcg ggaaagacca ggcgatcgat
     8041 tcgctggcga gaccgcggca ccgggctcga ggtccgatgc gaagctctcc tgtacgagga
     8101 attcccggtg gtggagtgga cgctctacct caaaaacacc ggtgtgactg aaactccgat
     8161 catctccgat ctgatgccgc tggatacgtg cgtcgcaagg ggagatgccg gatcgacgga
     8221 tttgagcgag ttcctgttgt accaccacgc gggaagcacg tgcacgccgg gcgactatcg
     8281 gccgttcgag tcggtgttgg cccccgcgac aacgaaaacc atcgccacat cgggcggcag
     8341 atcatcagat gagaacttcc cgtatttcaa cctcacgtac ccaatccgga acggcgccgc
     8401 gggaatggtg attgccatcg gctggccggg ccagtggaag gcgcagttcc tgcgggacga
     8461 cggtatcggg cttcatatca gcatggggca ggagttgacg cacttccgat tgcacccggg
     8521 cgaagaggtg cgcactcccc tgattgccat tcttttctgg ctatccagtg attccggggc
     8581 tggagtcggc agggaccgcc ttgccgatcg atggcggtcg cagaatctct ggcggcggtg
     8641 gatgcttgct tacaacgtgc cgaccgaggc cgacagacag ccgctgaaac cgcgcctgat
     8701 ggcctgcagt tcgcaccagt acggggaaat ggttgaggca gatgaagcca gtcagatcat
     8761 gttcattgat cggtatcttg aggaggggct gaacccggac tactggtgga tggatgcggg
     8821 atggtatccg aacgaatggg gctggccaaa taccggcacg tgggaagtag atacgaaacg
     8881 ctttccacgc gggttgcgcg cgattaccga ccacgcccat tccagaggcc ttcagacact
     8941 tgtgtggttc gaaccggaaa gggtcacgcc cggcacgtgg ctgtataccc accatccaga
     9001 atggctgctt gggaaggacg gagaacagaa gctcctcgac ctcggaaatc ctgccgcccg
     9061 cgaatggctg accgatcatg tggacaggat acttaccgag gaagggatag atctgtaccg
     9121 acaggatttc aacatggagc cgctcccgta ttggcgcgcg aacgatacac ccgaacgggc
     9181 aggaatttct gaaatgcgcc acgtggacgg ccttctcgag ttctgggacg aattgcggcg
     9241 gaggcgcccg gggctgttga ttgacacgtg cgcctctgga gggcgacgaa acgatctgga
     9301 aacgttgcga agggcggttc cgctctggag gagcgactac atccttgaac ccgtcggaca
     9361 acagtctcaa acgtggggta tcgctctgtg gattccatat ttcggaacag gaacaaatca
     9421 tgaagacgcg tacgggttcc gcagcgtcat ggtcccgaac gtcaacgcgt tgtgggacgt
     9481 gcgtcgccgg gatctcgact acgacttgat cagaaacctc gttgcgcagt ggcggggagt
     9541 cggggagctg tttggcggcg actattaccc cctcaccccg tacagccttg agaatgacgc
     9601 gtggatagga tggcaattcc accgacctga cctggaaaag ggggtgctga tgctcttccg
     9661 gcggccggaa agccctcgtg cgagtgaccg attccccctg tggggtctgg atgtggaagg
     9721 cagatatcga atcgtcgacc tcgacgtgcg cgaacgagtt tcggttctgg gcggggacga
     9781 gctcaccgcg accggcgttt cggtcgacat cgtcgccgct ccggggtcag caatgctcgt
     9841 gtacgaacgt gtggcttcgt aagttcggaa aggttgccac gtgaccactt tgccgcgaga
     9901 cccgcacgaa ggattcactc tcgagaacga cgtcctcgga gtgcgcctct ggggaccacc
     9961 cgcgtctcct gcgcttttgc tcggagcgtc tgatatctgg gaccgacgct ggtttgcaga
    10021 gcagcaaccc cttgttacgc tcgaaaaaat ccgggaattg gccttcgcgg atcgtctcgc
    10081 ggaaattgcc cgaagcccca acgaaacgat atatcatttg tatcagcggt acgacttccc
    10141 gtgtccgaag ccgggcgcac aaatcgtttt cctcacgccg ttcggaaccg atgcacacgc
    10201 cgaacacacc gggaattccc ttcaattgcg cgttaccgga ccgcggaaaa ccctgattgt
    10261 ggaaatctgg gtttcgctca cgcgacgcct tgttgtgtgc gaatgcacgc agcaaggcct
    10321 ggaacccacg gacctggccg tgcgggtgta ccgccaccgc gataccatcc tgcccgggga
    10381 gccgacagat ccgacaatcg gggggcaccc gtcggccatg gacttcgcgc cgtttcccat
    10441 gcccgatgcg ttttgcctgc ccataggcaa cggcggcggc gtgaagcaga cctttccgcc
    10501 cgacagcacg ttcgaagccg gttttgtatt cgcggctgcg gccagtgttg ttggtctgcg
    10561 cgcatcgctc catctggaca cttccgggag gggactcggc gcaccctact gggcagaaaa
    10621 agaaggtcgc ctcgaccacg ggacggtaaa gcgctacgcc ccgataaact cagccgaggg
    10681 cgcggcggcg actctggccc ttcacgagcc tggcgacagg ttttgcatcc tggctacggt
    10741 tgccacgtca caggactccc tggacccgat ccaggaagca gccaacacgc tcgtggaggc
    10801 ggagcggcag ggagtggcgt ttctgcgtgc ggagcagaaa aaggcccttg attcagcgcg
    10861 aagagcccgg cgggcgatcg cgactttgaa tggtcaaact gtcctgaacg cgccggcgac
    10921 gatactgcca aacctgcgaa gaaagggtgg gtactacggt gacatcccca tgtgcagcgt
    10981 ggggcccacg aaattctgct ttcaggatgc gggactctgg catgcggact ttcaccttaa
    11041 cgaaatcagg gcagagccgc tcctgacact cggaaggttc gaggacgtgc ggcttttcgc
    11101 ggacatgatt cgcgacctgc tgccccaggc tgaggaaaac gcggcggatg tgtacgggct
    11161 cccgggggca atgtatcctc tcgttcattt cccgcttcga tgccggggaa tcgcgcacac
    11221 caacctcaca tgggaacagg acatcgggtt gaacgggctc gtcgcaaaac ctctgtggct
    11281 tttctttcgc tacacggggg acaccgcgtt tctgcgagat acggcatacc cggtgatgcg
    11341 aagctgtgcg gatttttgca cggcgtacct ttcagaaggg gaagacggcc atctgcacat
    11401 tgtgccgacg gtgtctcccg agcactgggg gctcactgcg cggttcgagc gcaatcgaga
    11461 cagcacatcg gcactttcgc ttacgaagta tcttctgaac gcaacagccg aggcggccga
    11521 cctgttgcac accgattctc agcaagctga gagatggagg gccgcagccg cgcgactggc
    11581 gccgtaccca acgtgcgttg tcgacgggga agagatctgg accgacgtgg ccggcgctcc
    11641 tccgatcgag tataacatcc cggttcccct ttcgcccgtg ttctgggggg acgatgttgg
    11701 cctcgattca catcctgagg cgaaagcact ggcctatcgc acactgcgtc acatccgggt
    11761 atgggaacca caccggttct acctcgattc gtgcgtaagg ccgcggctcg gcgttcccag
    11821 tccgaacgcc ccgattgttc cggaaagcat gctcctttcc taccagagcc ttcacatttt
    11881 cccgggcgta cctccggatg ttccggttgc gatgcagaat ttcgcggcag agggcggatt
    11941 ccgggtatcg gcaacgaaaa cggaaacagg ccgcattgaa gaaatcatcg tctacagcgc
    12001 ctgtggcggg gtatgccgcc tcaccgaccc gagaaccggt gcgctgctcc aatacgaaat
    12061 gcaacccgac gagacactca gactgtaacg ccgggaaggc gctcaggttg acccgggagg
    12121 ccgccgcacc tataatgttg tcaagattgt cggcctcgcc acatcggggt ttgacgatgg
    12181 aattccgtcc ctgttgtgca aaaaactgaa agctccgccc tgatgagtct cctcgaagtc
    12241 ctccgccgca actggccgtt gaaagtcgcc tcgttcatag tcggttttac cttgtggctg
    12301 cacgtgaaaa ctgaccagac gtacgaacgg gttgtttcgc tgccgctgga aatcagggag
    12361 ccctcggggc gtttcgtcgt atcaaatgac gtaccgcgga ccgtgcaggt acggatctca
    12421 ggctcgggga aacagattct ggtcgggttg cgccacgcgc gcgtcgtcct gaagccgcga
    12481 gtaaatcgcc ccgaagcact cacgctggac atatccaccg acgacgtaca ggggttggat
    12541 ccgaaccgcg gaattgtcgt cacaaacatc gaggaaccac gatcggtatt gctggacttc
    12601 gatttcctcg aaatgcgtga ggctcaggtg ataccgaggg ttggtatcgg tgtaaagccc
    12661 ggctacacgg tcgtcggaga tgttcgcgta gacccaccga aggtaagatt gggggggccg
    12721 cgaaacgtca tcggtagtat tcgcaccgtc ctcacggaca gcgtggttct cagcgacgtt
    12781 tcctcagacg tcggcgaact ggtgacactg gagttgcccc gcgagatggg ccttgtggca
    12841 agccctgaaa cggtgcgggt gcgggcgacg gtccaggcgc ttgcagaacg gcgcttcagc
    12901 gacattccgg tagtcgtaac gaacctgccg aagagagtga acctgatcgc caatcccgcc
    12961 gttgtggcgg tggacctcac cggtggtgag cacgtccttg aaaatgtctc cgccgaccag
    13021 atttctgcag aggtggatta ccagcagagc gcgcagggag gaggcgcaga cctgccggtg
    13081 aaggtgtcgg ttccgccggg cgttcgtgtg aagcaaatca ccccggaaat cgtccgactc
    13141 gtgccaggcc aacgatcgga atctgtccga tgattgtcct gggcatcgag agttcgtgcg
    13201 atgaaacagc cgcagcggtg ttcgacacgg aatccggtct gcgctctaat gtaattgcct
    13261 cacaggcgga gcacatcccg ttcgggggcg tcgttccgga gatggcgtcg cgggcccaca
    13321 cggagcgcat tgttcccgtg gtccggcagg cactcgccga tgcaggcgtg accctcgacg
    13381 agattcatgc actcgccgtt acggcgggtc ccggtttgat cggttcgttg ctcgtcgggg
    13441 tgtctttcgc gaagggtgtt gccttcaccc gcaacctccc gctggttccc gtccatcaca
    13501 tcgaagcgca cgtgttcgcc agccgcatcg agcatcctga cctcgcgcct ccctttctcg
    13561 ctctcgtcgt gtctggtggg catacacaat tgatctgggt acccaaatgg ggcacctatc
    13621 gcgttctagg aacgacgcgg gacgacgccg tgggagaggc atttgacaag gttgcgaaaa
    13681 tcctcggcat cgggtacccc ggggggccac tgatagacag attagcccgt gacggtaacg
    13741 cggacggggt gaagttcccg aaggctttca tcaaagggga cccttttgcg ttcagtttca
    13801 gcgggctgaa aaccgccgtg ctgctggcag tggagaaaac gccggagaac gaactggaat
    13861 tgcgcaaagc ggacctcgcc gcaagtttcc agaacgcagc ggtcggtgca cttgtccaga
    13921 caacactccg ggccgcgcgg aaaatgagga ccaaacgcgt cgtccttgca ggcggagtgg
    13981 cttccaactc cgcgcttcgt cgcgagatgg aagccgcctg cgcccgaatc ggcattgagg
    14041 ttacgaggcc ttccccggtc ttttgcacgg acaacgcggc gatgactgcg tgtgccggcg
    14101 cattctggtt tgagcgcggg cgtcgagcgg ggtacaattt caacccgaac ccccgtctcc
    14161 cactcgacgc gttcggcaac gaatagagca cagcgagcac agaacgcaac aggaatcgtt
    14221 cgaatggcag gcgccaccaa agccaccgtg gaccttgaag cattgcgcgc aaacgtgcgt
    14281 ttcgtcagag aactcgtagg caaaggccgg ttgatacttg cgtgcgtaaa agcgaacgcg
    14341 tacggacacg gggcactccg gtgcgcgaac gcagcgctgc aagccggcgc agacgccctg
    14401 ggcgtcgcga gactcgatga agcgctggaa ttccgccaaa acggcatcgg cgggcgcatt
    14461 gtgctgatcg gacctgaatc acttgaagca accaatgact tggtacgagc ggacgttgaa
    14521 atcgcaatag actgcgccga tcggttggaa gctgtgcttc gagcgggcaa agcgtctcgg
    14581 cgtgttccga tcgtccacct cgcggttgat tccggtatgg cacgtttcgg tgcgtctttc
    14641 gaggaagcga gtcaactggc tgttcgcctg tcccaggcgc cggaagttag ttgggccgga
    14701 gtcatgacgc atttcccggt tgcggacact gatcaacaaa ggtccgttga gcagtggcgc
    14761 cggttccacg cacttctcag cggttggaaa acccgcggaa tagccgttcc gtgctgccat
    14821 gcggccaaca gcgcggcaat catggggtta cctgagactc acgcggacat ggtacgcccc
    14881 ggaatcatgc tttacggcat tccgcccgga acagcgcaca cctcggacaa attgcgcccg
    14941 gtccttcggt tggaaacgag cgtggttgct ctgcactggc gagaagaagg cgactatgtc
    15001 ggctacggcg agacattccg gtgcaaccgc cgcactctga ttgcaacgat ccccgccggg
    15061 tacggggacg gcatcccgtg gaccgcagga aaccgctcct gggttctcgt aaacggcaga
    15121 aaagcacctg tcgtgggccg aatcagcatg gaccagacca ctattgacgt taccgacctg
    15181 cgagaggtcc atctcggcga ccccgtggtg ctgatcggtt ccaagggcga ccaggccata
    15241 acggctaacg actgggcccg gtggtgcgga acgatcagct acgagatcac cacacggctg
    15301 cgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngactgg agacaggttt
    15421 gcttaccgag attgacactg tcagtgattt gtcacagatc gcgccccgag ttgcagaact
    15481 acttgtcggg caacgatatg cagtcatcgg tgcggttgtg agctgggcgg cggcccgtgt
    15541 agcatccctt ttggccggaa acggtaccgg cgtcacaggc atgacttcgc tatccggctt
    15601 cgggctgaga atagactatg agcatggcga gcccggcgta gatcgagtgg cagcctgcgc
    15661 cgaagcatac gcacggaaag gtggcccgct cgtgcttgtg ggagccggca ccgctttgca
    15721 tacgaacgtc gtcacgcggg agggcgtgta tctgggtggg gcgatcatgc cgggcttccg
    15781 gctcatgttc gaagctttga cggcgggaac agaccagata cgcgtagttt ccccctccgt
    15841 accttcgaga gtaatcggtc ggagtactcc ggaatgcgtg cagattggcg tgtaccattc
    15901 ctggctggga ggggcggtcc gcctggtccg aagaacctgc gaggaggtgg gaggacaccc
    15961 tgccttgtgg ctgaccgggg gacaggcaga atggctgaaa acggagttcc ctgaagccga
    16021 gtttgaccct gatctgacgc tgaaagggct cgcgcgggcc ttttgtggcg ccgatcaaca
    16081 aaattaccgc aattcgcaga atcgtactgc ctagattcat cgcgccgaaa tcgtctgtcg
    16141 ttttttcgtt gcaaaggagg ctcaaaaatg gaatatcgcg gcaagtatcc atatttcccc
    16201 tatgagaaga tctccaccta cccgatcaaa aaccggaaga acaaggttcg gcaggaggat
    16261 ctgtatttcc ccgaccgtgt gatggctatg acaccggagt tctcgcatcc ggagctcggg
    16321 aacatcgctg acacgatcgt gaaagcgcgc gacaaggggt acccggtgat atggcttatg
    16381 ggcgctcatc cggtgaaagc gggaatgtcg ccccttatca tcgacctgat gcagcggggc
    16441 atctgcaccc tcctgtgcgg caatttcgcg agcgcgatcc acgatttcga gctggcg
//
LOCUS       DAOSOO010000054        16391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3567_length_16391_cov_5.931072, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000054 DAOSOO010000000
VERSION     DAOSOO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3567_length_16391_cov_5.931072"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLT86_05705"
     CDS             complement(<1..239)
                     /locus_tag="PLT86_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOT36140.1"
                     /translation="MSASLRRLDREKRTIRVMIGMYCRAHHTPAVPLCPECAELLNYA
                     LQRIDRCRFGADKATCANCPVHCYKPAMRERIRTV"
     gene            complement(402..1319)
                     /locus_tag="PLT86_05710"
     CDS             complement(402..1319)
                     /locus_tag="PLT86_05710"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT36141.1"
                     /translation="MSSQNWYRGNTHTHTNVSDGNASLEYTAEWYHKHGYDWLVITDH
                     NKFTPVEEIPYHPETPFLLIAGCEISMSAEGKPVHVCAVNPRENPNSVAMPTIIQTLQ
                     AGVDRTNTLGGIPIINHPNWCWAFTEYHMARVTDWALFEIFNPSTDCNNWGGGGELSM
                     EERWDRMLSAGMRVFAVAADDSHTFVGEFYGHLSLPGLGWVTVRAPELTTDAICAALK
                     AGDFYASTEIELEDYEVTPEWIRVRIKQFDGYKYTTKFIGMNGKVLEERYGTEAVYRR
                     RGDEPYVRARIWSSNGGHAWCQPVFPDSV"
     gene            complement(1401..3041)
                     /locus_tag="PLT86_05715"
     CDS             complement(1401..3041)
                     /locus_tag="PLT86_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT36142.1"
                     /translation="MNRHILSVSVVSAFAVCVLVLSSCAHAPSVRTPHTSTTAQGTAT
                     RAGSFPINGIEAILDSTGLAEYAGEDDTVVESETDVEGIFRSAQERYEKAYECIASAR
                     AQIDTALGLLDEIRDTEDSTALTQRDEMILEFSHLMRQLGFVEQRGTVSMNGEIPLQM
                     NQFVEREIRSFQTVERSWFLGAYRRSGVYLPYIKARMREAGLPEQLAWLAFIESGYSP
                     RALSPARALGLWQFIPSTGYRYGMTRDRWIDHRMDFERSTTAAIAYLRDLHAMFGDWN
                     AALAAYNCGEGRVLRTINSQANQYMDDFWDLYVRLPQETARYVPRFHAVMAIMQDPLK
                     YGFSDLPQPEPPIAYDTLIVDRQVKLADIAERTGISADVLTRLNAELRTGITPQYPYR
                     LRVPVGTLDSVTVALNSAPTTSAPNLPDLITHRVKRGETIASIARKHRTTVTAIRQEN
                     GLRKNSRVRVGQILRIPVRSRASTPSQVASSSPRTARRNSNSHSSEGSPTTYVVRSGD
                     TLWSISQRYSLSVDELRNLNGLSRRSTIKPGQRLVVSR"
     gene            3294..4217
                     /locus_tag="PLT86_05720"
     CDS             3294..4217
                     /locus_tag="PLT86_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36143.1"
                     /translation="MNINEIRTACSQYRVVLEPDENTPEWWAGAPSVTRGDNGEFFLA
                     ARMREGKSPRGRRGYEVRILSSADGASFTPVNHIARDAAGVIGFERPALVRDPHSGAY
                     RLYGCCAFEDGWGIIRFQDAAHPKDIDPAKWQVVLKAENPADGFNHTVGYKDPFIFWD
                     RDKWRMFVIGYDRVERAYQFVSADGTKWEPASDRPALENTGWHSFFTRPACVLPLSVG
                     YLLVYEGSTILWRDPDYNIATGLAYSPDMETFIDLTPDEPLLKSTTAGPYHTWRYSHW
                     VHVGEEVYVYFEAARPNATNEIRLATFREGR"
     gene            4214..5398
                     /locus_tag="PLT86_05725"
     CDS             4214..5398
                     /locus_tag="PLT86_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT36144.1"
                     /translation="MNSKTSGPSVLVTDFFPRGYATQGELSYQQEIQNAIEAAAERKQ
                     SVLFPPMVYKLDESGVRLHSHLTVWMYGAVFVLDARCARDGQAFFGDDLTDLRICGGE
                     IVGRNDVWPAGVNIRGLYLTGNTSNVRVQDMHMRDLSSNGIGVFGTSEMPARDVWVTD
                     CVIEHCCNRYGDYMSKDAGPEKGSVREDQGLIAFYHVEDFVVRGCRFERSRSDGTHFY
                     RCRRGHFVHNRVYGAQMGGYFLETCEEVLASDNVILDNGSRGVTIERGSRNCILNGNV
                     VSNSGREGLWAPDSAGLIVTSNIFDRNGRKPNDLERGRIWNANITIDDDPGEPTHSPT
                     HDYLVAHNIITTTESQIAAIRIDAEVARGIVVRDNTLLGDNRQILVQGRNEGHVVLEG
                     NV"
     gene            5395..5787
                     /locus_tag="PLT86_05730"
     CDS             5395..5787
                     /locus_tag="PLT86_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOT36145.1"
                     /translation="MSSEAPSPRPRLLRWLLVTAGMLSVSLGFVGVFVPILPTTPFLL
                     LAAACFLRSSDRLYHWLMSHRVFGPYLQNYREHRALTLRAKVITTVGIWVSIGYAVVF
                     AAESVALRLALLVIACAVNAHILRLKTV"
     gene            5838..6938
                     /locus_tag="PLT86_05735"
     CDS             5838..6938
                     /locus_tag="PLT86_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36146.1"
                     /translation="MPFVRELGSERIECRDRVTGVHITQITSFPVMSNHFYIEHPSFT
                     SDCSTLVIRSQRVPARAAPWDLYACDADGLNLRQLTDEDDANNFALAHEGSLAWYQRG
                     TTLWSVDVGTGERREVCGGPPDVLPMGYWCGCASPDGKFYFGYGRRRSDDQGVVIRYR
                     TDGTEAVVLAADPHLCHLHASPGGHGISFGGVNERGEGVQYGMNHDGTNLRVLPTATL
                     SHFTWAGTTKRFVGAGMWERRVIHTIEEGQRDSSVVVEGSFFWHTGISWDAEWTVADT
                     NWPNEGLMIVNIRARQYARLCLTDNTAGHPQWTHAHPAFSPDGRRAVYTSDRTGMCQV
                     YVADVPEEFKAWVATPNPDAAPGGRLPEWRPE"
     gene            complement(7027..7788)
                     /gene="metW"
                     /locus_tag="PLT86_05740"
     CDS             complement(7027..7788)
                     /gene="metW"
                     /locus_tag="PLT86_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOT36147.1"
                     /translation="MSRTPYEWEDRLVALLLRHAAEGEAIHEKLARLVDILEAEEKAL
                     GRVTGKPPEKEGEQRYDHRLIEQHIPDGASVLDLGCGAGTLLSRLIQEKRVRGQGVEI
                     DNAKVLECVANGVPVFQTNLDHGLGMFPDQSFDFVVLEKTVQTLNRPLPVLQEMVRVG
                     KQGIVTFPNFGHWQMRLDLALNGRMPVTRAFPYEWYESPNIRPLTLNDFDACVQCQGM
                     RIVEGHAVVGNTVRPLEPDDNLFAEEVMLIIEKAA"
     gene            complement(7785..8996)
                     /locus_tag="PLT86_05745"
     CDS             complement(7785..8996)
                     /locus_tag="PLT86_05745"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOT36148.1"
                     /translation="MLDVNWARTVFPPGALDDSTSVGLTSAHTVTLADENSPFRLDTG
                     EFIAPVDVEYETYGTLSADRDNVVLVVHALSGDAHVAGWDYDAIQTGRKWRLRKPGWW
                     DTVVGPGKPLDTRRYFLICANVLGSCYGTTGPGSPNPRTGQPYGLSFPMVTVGDWVNL
                     QARLLDHLGIERILAVVGGSLGGQQVIEWMMAYPDRVEKAIVLAASAKLSAQGLAFNA
                     VGRQTIMSDPNFNGGNYYGGPVPAQGLSAARMLAHITYLSDAAMDMKFGRRYRNKTKP
                     DFDFGIEFEVESYLAYQGQSFVERFDANSYLYITRAMDYYDAAERWGGGNLVEACSRV
                     TADTMVVSFSSDWLYPPAQCRELAAAFCQCGKPVTYIEVPSSYGHDAFLVETEIVGKL
                     LADFLARERKR"
     gene            complement(8998..10311)
                     /locus_tag="PLT86_05750"
     CDS             complement(8998..10311)
                     /locus_tag="PLT86_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOT36149.1"
                     /translation="MSERTPHIETAALHAGQTPDPVTGSRAVPIYQTSSYVFNSTEHA
                     ADLFALKTFGNIYTRIMNPTTDVLEKRLAALDGGTGALAVASGQAAITLAVLNITQAG
                     QNIVSTSYLYGGTYNLFRYTMGRMGIQARFVDSFDPRNVERAIDDNTRLVYMESIGNP
                     KNNVDDFEAIAAIAHARGIPFIVDNTVSPPPIFQPFNHGADIVVYSLTKYIGGHGTSI
                     GGAIVEKGDFNWANGKFPEIAEPDPSYHGVNFWEAFGNHDKAAARGLSYILKARVQLL
                     RDLGPSISPFNSWLFIQGLETLPLRVRQHAKNAQELAEWLETHPAVAWVNYPGLKSHR
                     DNARAAKYMPEGPGAIIGFGIKGGREAGKKFIEAVKMISHLANIGDAKSLVIHPATTT
                     HQQLSPEEQLASGVTEDYIRFSVGLENVADIKADLDQALKASQEN"
     gene            10696..12366
                     /locus_tag="PLT86_05755"
     CDS             10696..12366
                     /locus_tag="PLT86_05755"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT36150.1"
                     /translation="MPTDIEIAQRTTLRPITEVAGALGLAQNDLELYGPHKAKISFDA
                     ISRISAHSRPGKLILVTAMTPTPAGEGKTTLAIGLSQALCRLGKKSLVALREPSLGPV
                     FGVKGGATGGGYSQVLPMEDINLHFTGDLHAVTTAHNLLIAMLDNHLHQGNALGVDVR
                     SICMNRTLDLCDRALRKVIVGTGGYANGVVRESGFEITAASEVMAILALSRDMADLKA
                     RLGRITVAATSSGRPVCASDLGAVGAMAATLRDAMKPNLVQTVEGTPALIHAGPFGNV
                     SFGCNSVVATRLGLAMSDYLVTEAGFGSDLGAEKFMNIKCRTAGVMPQAIVIAATIRA
                     LKYQGGADLKKLESKDLAALEKGMPNLYKHIENMQAHGVSPIVGLNRFATDDQEEISL
                     VLESLTAKGIKVALADVWARGGAGGEELAALVLDAVEKPSSPHFLYEDDLPLAEKIRK
                     IAVTIYGADGVTFAGAAKSKLARYEEQGYGKFPVCIAKTQNSLSDQPALKGRPSGFIV
                     TINDVKAATGAGFVIAYAGNILTMFGLPEHPAAERIDVNDSGEISGLF"
     gene            12407..13597
                     /locus_tag="PLT86_05760"
     CDS             12407..13597
                     /locus_tag="PLT86_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36151.1"
                     /translation="MTPRERYVRLFHFQPVDYVPDEEFGWWDETLTAWHKQGLPSEIN
                     DNWKGDFFFGFAQTDWAPVNQGLIPGFEHKVISEDENHVIFIDGSGVTQMANKGGRAS
                     IPKYIRFPLETRDDWEDFKRRMDPTDPRRIPSNIDEIVERLKTAENPVGIGMGSLFGW
                     IRNWMGFENLAMLTIDDPEWVEEMVDYLADFIIACAEPVLQKMEKAGVKLDVVSMWED
                     ICFNNGPMVSPTFFRAVVTPRYKRITDRVRKVGCDLTYLDCDGDVTQLVGPWLEGGVN
                     IMFPIEIRGGSDPQQMRDTYGHEVLLKGGVDKMALIEGKEAILADLKRLDPLVRDGGF
                     IPHVDHRVPPDVTYENYLYYLREKRAWLGIPEPPPYEERLEELRKRDEIAARNFTRTD
                     TVKT"
     gene            13635..14471
                     /locus_tag="PLT86_05765"
     CDS             13635..14471
                     /locus_tag="PLT86_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT36152.1"
                     /translation="MATRSIIKIDQSLCDGCEQCIPNCPEGAIQVIDGKARLVSDLFC
                     DGLGACIGHCPQGAITIEEREAEPYDERRVMANVVQQGTNTIAAHLVHLRDHDEFDLL
                     AEALAYLKENGIPVPPTGGGKVIATMAPAACPGSKTVDLREAPAQVSREAVTGLPTQL
                     RQWPVQITLVPPHAPFLNGCDLLIAADCVPFAYPDFHQELLKGKVLLVGCPKLDDVRP
                     YEEKLTQIFAQNEVKSITYARMEVPCCGGFVPVIRRALAASGKEIPFQDIVIGIRGNR
                     LS"
     gene            14586..15542
                     /locus_tag="PLT86_05770"
     CDS             14586..15542
                     /locus_tag="PLT86_05770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36153.1"
                     /translation="MGSDSTATALSASVVIMTNEREDDLKRSVTALLAQADPSWELIV
                     VDDTPVLSPAMEAFLAENATRFRYVRLTTKNLPRAGNVGITLARGRIVIFVDDDVIPC
                     EGFVARHVAAYTDPSIGGVMGRIRQHNFPEWEPDPSPRDMGFNAFWHKHFNWKTRHPI
                     AMTRGCNMSFRRDVLFEVGGCDEQFVAEGMRWDTDLGYCVRQAGYTIMYEPDAWLEHL
                     YVPRGGARTRGLDLKRTEKYYQNNFYFLCKHRPPFWSAAYYTLAVFLAGYVRKEVIRQ
                     PALLWQIPRMMCRGMRSARRLARDIPATFLPAPVPDAAPVTP"
     gene            15539..16033
                     /locus_tag="PLT86_05775"
     CDS             15539..16033
                     /locus_tag="PLT86_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36154.1"
                     /translation="MTGEIPPVGTHTHVQKLLLSWGRQTGHRVWVAKGDRGRYSEGRF
                     LADGCIERIPTFMPARVLSILENVDVVWFPPSGAVPVALFEVEHSTAILGGLMRMNDV
                     VTTLVPPLEGWRFFVVAPARRISRFNGELARPTFQASGLARVCRFLSYDHVVEGMRNN
                     LPLR"
     gene            16039..>16391
                     /locus_tag="PLT86_05780"
     CDS             16039..>16391
                     /locus_tag="PLT86_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT36155.1"
                     /translation="MALHPAIQNLGVLVKPASARCNLACTYCFYHERESDPYARVGSR
                     RMSESVLERFLAEYLPLAGVQPTLGWQGGEPLLAGLRFFERMIELQEKLRDRSQTISH
                     AIQTNGTLIDDDWAR"
BASE COUNT         3145 a         4619 c         5282 g         3345 t
ORIGIN      
        1 atgacggtgc ggatgcgctc ccgcatggcg ggtttgtagc agtgaaccgg gcagttggcg
       61 caggtggctt tgtccgcgcc gaagcggcac ctgtcaatcc tctggagcgc gtagttgagg
      121 agttcggcgc actcaggaca gaggggaacg gcgggtgtgt gatgagcgcg gcagtacatc
      181 ccgatcatca cgcggattgt gcgtttttcg cggtcgaggc ggcggagcga agcggacatg
      241 gaacgggtgt cattcccgta agaagtttgt taccacgaac cacacgaacg acacgaaagg
      301 attactccgc accccggtct ccgagcaagc cgcgtggata acgcgagggc gcttcgacag
      361 gcttccgcgc ggtggacgca ggcaacgcct ccggttctga atcacacgct gtcgggaaac
      421 accggctggc accaggcatg gccaccgttg gatgaccaga ttcgcgcgcg aacgtacggc
      481 tcgtcgccac gccggcggta aaccgcttcc gtgccgtacc gctcctcgag caccttcccg
      541 ttcatgccga tgaacttcgt ggtgtatttg tagccgtcga actgcttgat gcgcacgcga
      601 atccactccg gcgtgacttc gtagtcttcc agctcaattt ccgttgaggc gtaaaagtcg
      661 cccgccttga gcgcggcgca gatggcgtcg gtagtgagtt ccggcgcgcg cacggtcacc
      721 cagccgagcc ccggaaggga caggtggcca tagaactccc cgacgaatgt gtgggaatca
      781 tctgccgcga ccgcaaagac gcgcattccg gcggacagca tgcggtccca gcgctcttcc
      841 atgctgagtt ccccgccgcc gccccagttg ttgcagtcgg tgctggggtt gaatatctcg
      901 aacagcgccc agtcggtgac gcgtgccatg tggtattcgg tgaatgccca gcaccagttc
      961 gggtggttga tgatggggat tccgccgagg gtatttgtgc ggtccacccc ggcctgcagt
     1021 gtctggatga tggtcggcat ggcgacgctg ttcgggtttt cccgtggatt gaccgcgcac
     1081 acgtgcaccg gttttccctc ggcgctcatg ctgatttcgc acccggcgat aaggagaaac
     1141 ggagtctcgg ggtggtacgg gatctcttcg accggtgtga atttgttgtg atcggtgatt
     1201 acgagccagt cgtacccgtg tttgtggtac cactcggccg tgtattcgag ggaggcgttt
     1261 ccgtctgaga cgtttgtgtg cgtgtgggtg ttaccgcggt accagttctg ggaagacatc
     1321 ggggctcctg gaaaaggaga gaggcgcgcg gtgcgcccct caagttcgaa agtgtgaagg
     1381 ttgcagccat tacgaacaaa tcagcgactg acgaccagcc gttgccccgg cttgatcgtg
     1441 gagcgtcggc tcaacccgtt gaggtttcgg agttcatcca cggagagcga gtagcgttgc
     1501 gagatggacc agagtgtgtc gcccgacctg acgacgtagg tggtggggct gccctcactc
     1561 gagtgtgaat ttgaattcct ccgggccgta cggggggacg aagacgcgac ctgcgaaggg
     1621 gttgacgcgc gcgaacggac cggaatgcgg agtatctgac cgacgcggac gcgggagttc
     1681 ttcctgaggc cgttttcctg ccgtatggcg gtgactgtgg tgcggtgttt ccgtgcaatc
     1741 gaggcgattg tctcgccgcg tttcacacgg tgggttatca ggtccggaag gtttggcgcg
     1801 ctggtggtgg gcgccgaatt cagggcaacg gttaccgaat caagagtgcc taccggaacg
     1861 cgcaggcggt aggggtactg aggcgtgatg ccggtgcgca actcggcgtt caggcgggtt
     1921 aatacgtccg cgctgatgcc agttcgctcg gcaatatctg caagtttcac ctggcggtcg
     1981 acgatgagcg tatcgtatgc gatgggcggc tccggctggg gcaggtcgga gaacccgtat
     2041 ttcaggggat cctgcatgat cgccatgact gcgtggaacc ggggtacata gcgggcggtt
     2101 tcctgcggca ggcggacgta gaggtcccag aaatcgtcca tgtactggtt tgcctggctg
     2161 ttgatggttc gaaggacgcg tccctctccg cagttgtagg cggccagcgc ggcattccaa
     2221 tcgccgaaca tggcgtgcag atcgcgcagg tatgcgatgg ccgcggtggt ggagcgctca
     2281 aaatccattc tgtggtctat ccagcgatcc cgcgtcatgc cgtagcggta tccggtggag
     2341 gggatgaact gccacagccc gagcgcgcgg gcgggagaaa gcgcccgggg ggagtatccg
     2401 ctttcgataa aggcgagcca cgcgagttgc tcgggcagtc cggcttcgcg catccgcgcc
     2461 ttgatgtagg ggaggtacac gccgctgcgc cggtaggcgc cgaggaacca tgatcgctca
     2521 accgtctgga aggagcggat ttcccgttcg acgaactggt tcatctgaag cgggatttcc
     2581 ccgttcatgc tgaccgttcc gcgttgctcc acaaagccca gctgacgcat caggtgggag
     2641 aactcgagga tcatctcgtc gcgctgggtg agcgcagtgg agtcctcggt atcgcggatt
     2701 tcgtccaaca ggccgagggc ggtgtcgatc tgggcccgcg cgctggcgat gcactcgtac
     2761 gctttttcgt agcgttcctg ggcagagcgg aagattccct ccacgtcggt ttcgctttcc
     2821 accacggtat cgtcctcgcc ggcgtattcc gccagtccgg tcgaatccag aatggcttcg
     2881 attccgttga tggggaagga tcctgcgcgg gtggcagttc cctgagcggt tgtggaggtg
     2941 tgcggagttc gtacggatgg ggcatgtgca caggaagaca gtaccagaac acaaaccgcg
     3001 aacgcactca ccacactcac cgagaggata tgtctgttca tcacaggctt ccgtcgggag
     3061 atgatggccg taaccatcag atggacaagt ggtttaccga aggcggtcac aacagccgca
     3121 gacgatgggg cactcaagaa acatagaact gaggcgcgcg gttggcaagt gttagccgga
     3181 attcggcctg cggcgggata ggggttggcg ggttgcgggc gcgctattag gtacaatagg
     3241 gcctattcag tcattgccag gcaggcacaa cgaattgatg gaagggaaca accatgaata
     3301 tcaatgagat ccgcacagcg tgttcgcagt accgcgtggt gcttgagcct gacgagaaca
     3361 ctccggaatg gtgggccggc gcacccagcg taacgcgagg agacaacggc gagttctttc
     3421 tggccgcacg catgcgcgag gggaaatcgc ctcgaggtcg gcggggctat gaggtacgga
     3481 ttctttccag cgcggacggg gcgagcttta cgcccgtgaa ccatatcgcc agggacgcag
     3541 cgggggtcat cgggttcgag cgcccggcgc ttgttcggga cccgcacagc ggggcgtacc
     3601 gtctttacgg gtgctgcgcg ttcgaggacg ggtggggcat catccggttt caggacgcgg
     3661 cccatcccaa ggatatcgac ccggcgaaat ggcaggtggt gctgaaggcg gaaaaccccg
     3721 cggacgggtt caaccatacc gtgggctaca aggacccgtt catcttctgg gatcgcgaca
     3781 agtggcggat gtttgtgatc gggtacgacc gggttgagcg cgcctatcag tttgtcagtg
     3841 cggacgggac aaagtgggag ccggccagcg accgtccggc gcttgagaac accgggtggc
     3901 acagcttttt cacgcgcccg gcatgtgttc tgccgctttc cgtcgggtac ctgctggtgt
     3961 acgaagggtc gactattctg tggcgggacc ctgactacaa tatcgcgacg ggcctggcgt
     4021 attcgccgga tatggagact ttcattgacc tcacgccgga tgagccgctg ctgaaaagca
     4081 cgacggccgg gccgtatcac acctggcggt attctcactg ggtgcacgtc ggggaagagg
     4141 tgtacgtcta ttttgaggcg gctcgtccca acgcaacgaa cgagattcgc cttgcaacct
     4201 tcagggaagg aagatgaaca gcaaaacttc ggggccgagc gtgctggtca cggacttttt
     4261 ccccagggga tacgcgacac agggggaact gagttaccag caggagatcc agaacgcgat
     4321 cgaggcggca gcagaacgga agcagtctgt cctgtttccc ccgatggtct acaagctcga
     4381 cgaatcaggc gtgcgtctgc actcgcacct caccgtatgg atgtacggcg cagtgtttgt
     4441 tctggatgcg cgttgcgcga gagacggcca ggcgttcttc ggcgacgact tgacggacct
     4501 ccgcatctgc gggggagaaa tcgtcggccg gaacgatgtc tggcccgcag gggtgaacat
     4561 tcgcgggctg tatctcaccg ggaacaccag caacgtcagg gttcaggaca tgcacatgcg
     4621 ggacctatcc agcaacggta ttggtgtgtt cggtacttct gagatgccgg cccgtgatgt
     4681 atgggtgacg gactgcgtca tcgagcactg ctgcaacagg tacggcgact acatgagcaa
     4741 agatgccggt cccgaaaagg gaagtgtgcg cgaggatcag gggctgatcg cgttctacca
     4801 cgtcgaggac tttgtggtgc gagggtgcag gtttgagcgc tcccggtccg acgggacgca
     4861 cttctaccgt tgccggcgtg gtcatttcgt gcacaaccgg gtttatggcg cgcagatggg
     4921 cgggtacttc ctcgagacgt gcgaggaggt gcttgcttcg gacaacgtga ttctcgacaa
     4981 cggctcccgc ggagtcacca tagagcgcgg gtcccggaat tgcatattga acgggaatgt
     5041 ggtgtccaac agcgggcgag agggcctctg ggctccggac agcgcgggtt tgattgtcac
     5101 gagcaacata ttcgatcgga acggccgcaa gccgaacgat ctggagcggg gccgcatctg
     5161 gaacgcgaac atcacaatag acgacgaccc gggggaaccg acccattcac ccacgcacga
     5221 ttaccttgtt gcacacaaca tcatcaccac gacggaaagc cagatcgcgg ctatccgcat
     5281 tgatgcggaa gttgcccgcg gcattgttgt gcgggacaac acgttgctcg gagacaaccg
     5341 gcagatactc gttcagggac gtaacgaggg gcacgttgtt ctggagggga atgtgtgagt
     5401 tcagaggcgc catccccgcg gccccggctt ttgcgatggc tgcttgtgac tgccggaatg
     5461 ctgtccgtat cgctcggatt cgtgggtgtc ttcgtgccga ttcttcccac gacgcccttt
     5521 ctccttctgg ccgcggcgtg tttccttcgg agctccgacc gcctttatca ctggttgatg
     5581 tctcaccgcg tgttcggccc gtacctgcag aactacaggg aacaccgcgc gctcacgctg
     5641 cgtgcgaaag tcattaccac cgttgggatc tgggtgagta tcgggtatgc agtagtattc
     5701 gccgcagaaa gcgttgctct tcgtctcgca ctgcttgtga ttgcgtgcgc agtgaacgcg
     5761 cacattctgc gcctgaaaac ggtgtgacgg cttccggcgc agccagaagg tgcacccgag
     5821 accggggggg aacacagatg ccttttgtcc gcgaactcgg gagcgagcgg attgagtgcc
     5881 gcgatcgtgt caccggtgtt catatcacac agatcacaag ctttccggta atgagcaacc
     5941 acttctatat cgaacatccg tcgttcacct ccgattgctc caccctcgtg attcggtcgc
     6001 aacgtgttcc ggcgcgcgcg gctccgtggg acctttacgc atgtgacgcc gatgggttga
     6061 acctgcggca acttaccgat gaagacgacg caaacaattt cgcgctcgcg catgagggtt
     6121 ctctggcgtg gtaccagcgg ggaaccacgt tgtggagcgt tgacgtgggc accggggagc
     6181 ggcgagaggt atgcggcggg ccacctgacg ttcttcccat ggggtactgg tgcggctgcg
     6241 caagccccga cggcaagttc tacttcgggt acggaaggcg tcgatcggat gatcagggcg
     6301 tcgtaattcg ctaccgcacc gatgggacgg aagcagtagt tcttgccgcc gaccctcacc
     6361 tgtgtcatct gcacgcgagc cccggagggc atgggatatc atttggcggc gtgaatgagc
     6421 gcggcgaggg cgtgcaatac ggaatgaacc atgacggcac gaatctgcgt gtattgccca
     6481 ccgcgacgct ctcgcatttc acctgggcag gcacaacaaa gcggttcgtc ggtgcaggaa
     6541 tgtgggagcg cagggtcatc cacacgattg aggaggggca gcgcgattcc tcggttgttg
     6601 tggaaggttc gttcttctgg catacgggca tttcgtggga cgcggagtgg acggttgccg
     6661 acacgaactg gcccaatgag ggcctgatga tcgtgaatat ccgtgcgcgg cagtatgccc
     6721 gtctttgcct gacggacaac accgcggggc atccgcagtg gacgcacgcg caccccgcgt
     6781 tcagccctga cgggagacgt gcggtctata ccagtgaccg gacggggatg tgtcaggtgt
     6841 acgtcgcgga cgtgccggag gaattcaaag cgtgggttgc gacgcccaac ccggacgccg
     6901 ctccgggcgg acggctgccc gagtggcgcc cggaatgatt tgaacgcccg gggccggggc
     6961 gacgtacatg agaaaagggc gcacagaccg cgtccttttc gttccgtgcc ccgctgattg
     7021 acgaccttac gccgcctttt cgatgatcag cattacctct tccgcaaaca ggttgtcatc
     7081 cggttccagg gggcgcaccg tattccccac caccgcatgg ccctcaacga tccgcatgcc
     7141 ctgacattgc acacaggcgt cgaagtcgtt cagcgtcagc ggccggatgt tgggcgattc
     7201 ataccactcg taggggaagg cgcgggtaac cggcatcctg ccgttgaggg caaggtcgag
     7261 ccgcatctgc cagtggccga aattcgggaa tgtgacgatc ccctgttttc ccacccggac
     7321 catttcttgc agcacgggga gcggccggtt gagcgtctgc accgtttttt ccaacaccac
     7381 gaagtcgaaa ctctggtcgg ggaacatgcc gagaccgtga tcgaggttgg tctggaatac
     7441 gggaacgccg ttggcaacgc attccagcac tttcgcgttg tcgatctcga cgccctggcc
     7501 acgcactcgt ttttcctgaa tgagacggga aaggagagtt cccgccccgc aaccgaggtc
     7561 gagcactgag gcgccatcgg ggatgtgttg ctcgatgagg cggtgatcgt accgctgttc
     7621 gccttctttt tccggcggtt tgccggttac gcgcccaagg gccttttcct ccgcttcgag
     7681 tatgtcaaca aggcgagcga gtttttcgtg aatcgcctcg ccttcggcgg catgtcgcaa
     7741 caggagcgca acgaggcggt cttcccactc gtacggcgtt ctgctcatcg tttccgctcc
     7801 ctggcgagga aatcggccag cagtttgcct acgatttccg tctccacgag gaatgcgtcg
     7861 tgcccgtatg aagaaggcac ctcgatgtac gtaacgggtt tcccgcactg gcagaatgcg
     7921 gccgccagct ccctgcactg ggcgggtggg tacagccagt cggaggagaa gcttaccacc
     7981 atggtgtctg cggtcacgcg ggaacaggcc tccaccaggt ttccgccgcc ccaccgttcg
     8041 gcggcatcgt agtagtccat ggcacgtgtg atgtagaggt agctgttcgc gtcaaagcgc
     8101 tccacgaacg actggccctg gtaggcgagg taactctcga cctcgaattc gatcccgaaa
     8161 tcgaaatcgg gttttgtttt gttgcgatac cggcgcccga atttcatgtc cattgccgcg
     8221 tcgctgaggt acgtgatgtg cgccagcatg cgcgccgcgg agagcccctg cgccggcacc
     8281 gggccgccgt agtagttgcc cccgttgaaa ttcgggtcgc tcatgattgt ctgccgaccg
     8341 acggcattga acgccagacc ctgcgcgctg agtttggccg atgcggcaag cacgattgcc
     8401 ttttcgacgc gatcgggata tgccatcatc cactcgatca cctgctgtcc gccgagagac
     8461 ccgccgacaa cggccagaat ccgctcgata cccagatgat caagcaggcg agcctgaagg
     8521 ttgacccagt cgcccacggt taccatcgga aacgacaggc cgtacggctg accggtgcga
     8581 ggatttgggg agcctgggcc ggtggtgccg taacaacttc cgaggacgtt ggcacagatg
     8641 aggaagtacc gccgggtatc cagcggtttt cccggtccca cgactgtatc ccaccagccg
     8701 ggtttgcgca gacgccattt ccgaccggtt tgaatcgcgt cgtaatccca ccccgctacg
     8761 tgggcatcgc cggaaagcgc gtggaccacc agaaccacgt tatccctgtc ggcgctcaga
     8821 gttccgtaag tctcgtattc gacgtcgaca ggggcgatga actccccagt atccagacgg
     8881 aacggggagt tttcatccgc cagcgtgact gtgtgcgcgc tggtcagccc caccgaggtg
     8941 gagtcgtcga gcgcgccggg gggaaacacg gttctggccc agttcacgtc gagcacgcta
     9001 gttctcctgg cttgctttca gcgcctgatc aaggtccgct ttgatgtcgg caacgttttc
     9061 cagaccgacc gagaagcgga tgtaatcctc cgtcacaccg ctcgccagtt gctcttccgg
     9121 agaaagctgc tggtgcgttg tggttgcggg atggatgacg aggctcttcg cgtccccgat
     9181 gttcgcgagg tggctgatca tcttgaccgc ttcgatgaat ttctttcctg cttcgcggcc
     9241 cccttttatg ccgaacccga tgattgcacc ggggccttcg ggcatgtatt tcgctgcgcg
     9301 cgcattatcg cggtggcttt tgagcccggg gtagttcacc catgccacgg ctggatgagt
     9361 ttcgagccac tccgcgagtt cctgcgcgtt tttcgcgtgt tgccgcacac gcagagggag
     9421 cgtttcgagg ccctggataa acagccacga attgaacgga gaaatgctgg ggccgaggtc
     9481 gcgcagcaat tgaacccggg ctttcaagat gtacgacagg cctcttgcgg ccgctttgtc
     9541 gtggttgccg aacgcttccc agaaattgac gccatggtac gagggatcgg gttcggcgat
     9601 ttctggaaac ttgccgtttg cccagttgaa atcccctttt tccacaatcg cgccgccgat
     9661 gctggtgccg tgcccgccga tgtatttcgt caaggaatag acgacgatgt cggcaccatg
     9721 gttgaacggc tggaaaatcg gcggcgggct gaccgtgtta tccacgatga acgggatgcc
     9781 gcgggcatgc gcgatggcgg caatggcctc gaaatcgtcc acgttgtttt ttggattacc
     9841 aattgactcc atatagacga gtctcgtgtt gtcatcaatc gcccgttcga cgttccgggg
     9901 atcgaaggaa tcgacgaaac gcgcctgaat gcccattctg cccattgtgt agcggaaaag
     9961 gttgtaggtg ccaccgtaga ggtagctggt ggagacgata ttctgccccg cctgcgtgat
    10021 gttgagcacc gcaagcgtga tagcggcctg tccggaagcc accgccagcg ctcccgtgcc
    10081 accatcaagg gccgcgagac gtttttccag cacgtcggtg gtcgggttca tgatgcgggt
    10141 gtatatgttg ccgaaggttt tcagcgcaaa cagatcggcg gcgtgttcgg tcgagttgaa
    10201 cacatagctg ctggtctggt aaatggggac ggcacgggaa cctgtcaccg ggtcgggagt
    10261 ctgtccggca tgcaatgcag ccgtttcgat gtgcggtgtt cgctcgctca tggcgggaat
    10321 ctcctgtttc gcatacagat gcgatttgcc gcgtttgaat gcgactttgg tgcaacacgg
    10381 caccggtact attgatgata cggactgggc gcggtgctcg tccatatcgg gttgggtttt
    10441 gcggcggtcg gtagacatcc gatgcggcac ccaaggcatg gagttcgaag tcgcgtccca
    10501 cacgcgatac ccgatgatct gccggaagcc ccgcttccaa acctgaacac atgctgcgtc
    10561 gaagaaggtt ccccgccccc cgtggtttgg tgcggatgcg ggcaagccgt accttgattg
    10621 tcttacgacg tgtaactcaa ttacttcgtt tgtgcccgcg ttcgtgtccc gttccgtatc
    10681 aggggttgta caccaatgcc taccgacatc gagattgcac agcgcactac gctgcgcccc
    10741 atcactgaag ttgccggtgc tcttggtctt gcccagaatg atctggagtt gtacgggccc
    10801 cataaggcaa aaatcagctt tgacgccatt tcacggatca gcgcgcacag ccgaccgggg
    10861 aagctcattc ttgtaaccgc catgacaccg accccggccg gcgaggggaa aaccaccctt
    10921 gcgatcgggt tgagccaggc gctgtgccgc ctcgggaaga agtcgctcgt ggcgttgcgc
    10981 gagccttctc tcggaccggt tttcggtgtg aaagggggcg ccaccggcgg cggttattcg
    11041 caggtattgc caatggagga catcaacctg cacttcaccg gcgacctcca cgcagttacg
    11101 acggcgcaca acctgctcat cgcgatgctg gataaccact tgcaccaggg gaacgcactc
    11161 ggtgttgatg tgcggtctat ctgcatgaat cggacgctgg acctgtgcga ccgcgcgctg
    11221 cggaaggtga tcgtgggtac tggcggctat gccaatggcg tcgtgcgcga gagcgggttt
    11281 gagatcactg cggcaagcga agtgatggcg attcttgctc tttcgcgcga catggcagac
    11341 ctcaaggcac gcctgggaag gattacggtc gcggcgacga gttcgggccg tccggtatgc
    11401 gcatctgacc tcggggcggt cggcgcgatg gctgcgacat tgcgcgatgc gatgaagccg
    11461 aatcttgttc agacggtaga aggcacgcct gcactcattc atgccggacc gttcggcaac
    11521 gtgtcgttcg ggtgcaacag cgtggttgcc acgcggttgg gactggcgat gagtgattat
    11581 ctggtgactg aggcggggtt tggcagcgac ctcggcgccg agaagttcat gaatatcaag
    11641 tgccgaaccg cgggcgtgat gcctcaggcg atcgtcatcg cggcgacgat ccgagcattg
    11701 aagtatcagg gtggcgcgga cctcaagaag ctggagtcga aggacctggc cgctcttgag
    11761 aaaggcatgc cgaacctgta caagcacatt gagaacatgc aggcgcacgg tgtttcgccg
    11821 attgtggggt tgaacaggtt tgccacggac gaccaggagg agatatccct cgttctcgag
    11881 tcgctcacgg caaaagggat caaagtggcg ctggcggacg tctgggcaag aggcggcgcg
    11941 ggcggcgagg aactggccgc gctggtgctg gacgcggtgg agaaaccgtc gtccccgcat
    12001 ttcctgtatg aggatgacct gccgctcgcg gagaagatca ggaagatcgc ggttacgatt
    12061 tacggggcgg acggggtcac gtttgcgggc gcggcgaaat cgaagctggc gcgctatgag
    12121 gagcaggggt acgggaaatt ccctgtgtgc atcgcgaaga cgcagaactc tctttccgac
    12181 cagcctgcgc tgaaggggcg ccccagcggg ttcatcgtga ctatcaacga cgtgaaagcg
    12241 gccaccggcg ccgggtttgt aattgcgtat gcagggaaca tcctcaccat gtttggcttg
    12301 cccgagcatc cggcggccga gaggatcgat gtcaacgaca gcggagaaat cagcggattg
    12361 ttctgaaacg ggagatggat tccaacttcg aaccagaggt gcaccgatga caccacgtga
    12421 acgctacgtc aggcttttcc acttccaacc cgttgactac gttccggatg aggaattcgg
    12481 ctggtgggat gagacgctga ccgcctggca caagcagggt cttccgagcg agataaacga
    12541 caattggaaa ggcgacttct tcttcggatt cgcgcagacc gactgggcgc cggtgaatca
    12601 ggggctgata cccggattcg agcacaaagt catcagtgaa gatgagaatc acgtcatttt
    12661 catcgacggg tccggcgtga ctcagatggc gaacaagggc ggcagggcgt ccattccgaa
    12721 gtacatccgg ttcccgctgg agacgcgcga tgactgggaa gacttcaagc gccgcatgga
    12781 ccccaccgat ccgcggagga tcccgagcaa cattgatgaa atcgtggaac gtctcaaaac
    12841 cgcagaaaat cccgtcggca tcggcatggg gagcctgttc ggatggatcc gcaactggat
    12901 ggggtttgaa aaccttgcga tgctgaccat tgacgatcct gaatgggtcg aggagatggt
    12961 ggattacctt gcggatttca tcatcgcatg cgccgaaccg gtgctccaga agatggaaaa
    13021 ggccggcgtc aaactcgatg tggtgtccat gtgggaggac atctgcttca acaacggtcc
    13081 gatggtcagc ccgacgttct tccgggcagt cgtgacgccg cggtacaaaa gaatcacgga
    13141 ccgcgtgcgc aaggtcgggt gcgaccttac gtacctcgat tgcgacggcg acgtgactca
    13201 gctcgtaggt ccgtggctcg agggcggcgt gaacatcatg tttcccattg agattcgcgg
    13261 tggctctgat cctcagcaga tgcgcgacac gtacgggcac gaggttctgc tcaaaggcgg
    13321 cgtggacaag atggcactga tcgagggcaa agaggcgatc ctggccgacc tgaaacggct
    13381 ggatccgctg gtccgcgacg gtgggttcat cccgcacgtg gatcaccgcg tgccgcccga
    13441 tgtcacgtac gagaattacc tctactatct gcgggagaag cgagcgtggc tgggcatacc
    13501 cgagcccccg ccgtacgagg aacgtctgga agaactgcgg aagcgtgacg aaattgccgc
    13561 gcggaacttc acgcgaacgg acacggtaaa gacgtagtgg aaaaccacca cgccggtttg
    13621 ttcgcggagg cacgatggca acacgaagta tcatcaagat cgaccagagt ctgtgtgacg
    13681 gctgcgagca gtgcataccc aactgccccg agggcgcaat ccaggtcatt gacgggaaag
    13741 cgcgtctcgt cagcgatctt ttctgcgatg gtctcggggc atgtatcggg cactgcccgc
    13801 agggtgcaat tactatcgag gaacgggagg cggagccgta cgacgagcgg cgcgtgatgg
    13861 caaacgtcgt acaacaggga acgaacacga ttgctgcgca cctcgtccat ctgcgcgacc
    13921 acgacgagtt cgacctgctt gccgaagcgc ttgcgtatct gaaggagaac ggtattcctg
    13981 tgccgccaac cggaggcggg aaggttatcg ccaccatggc tcccgcggcg tgtccggggt
    14041 caaaaacagt ggacctgcgc gaggcgcccg cccaggtttc ccgcgaagcc gtaacggggt
    14101 tgcccaccca actgcggcaa tggccggtcc agataaccct ggtgccaccg catgcgccct
    14161 tcctcaacgg gtgcgacctt ctgatcgcgg cagattgtgt tccgttcgca taccccgatt
    14221 ttcatcagga gcttttgaag ggcaaggttc ttcttgtcgg gtgtccgaaa ctggatgacg
    14281 tgcgtccgta cgaggagaag cttacgcaga tttttgctca gaatgaggtc aaatccatta
    14341 cgtatgcccg tatggaagtt ccctgctgcg ggggctttgt tccggtgatc cgccgcgcgc
    14401 tggccgcgag cgggaaggaa atcccgttcc aggacatcgt gatcggaatc agaggaaacc
    14461 ggttgagttg agcgcgaaga cgcgttcggt actcacgggg cgtggccgat gccacgcccc
    14521 gtgatatttt tagactgtcc gatctgtttc cagtgtcctt catccgccct caccgtaagg
    14581 ttgccatggg ttccgattcc accgccactg cgttgtcggc ttccgtcgtc attatgacga
    14641 atgagcgcga agatgacctc aaacgctcgg taacggcgct actcgcgcag gccgatccgt
    14701 cctgggaact catcgtagta gatgacacgc ctgtgctttc gcctgcgatg gaagcatttc
    14761 tcgcggaaaa cgccaccagg ttccggtatg tccggctcac gacgaagaac cttccgcgtg
    14821 ccggcaacgt aggcatcacg ctggcccgcg gaagaatcgt gattttcgtc gacgacgatg
    14881 tgattccgtg tgaaggcttt gtcgctcgcc acgtagccgc ctacacggat ccttccatcg
    14941 gcggcgtgat gggacgcatc cgccagcaca atttccccga gtgggaacca gaccccagcc
    15001 ctagggacat gggcttcaac gccttctggc acaaacactt caactggaag acgcgtcatc
    15061 caatcgcgat gacgcgtggc tgcaacatgt ccttccggcg agacgtgctg ttcgaggttg
    15121 gcggatgcga cgagcagttc gtggcggagg ggatgcggtg ggacaccgat ctggggtact
    15181 gcgttcggca ggcgggctac acgatcatgt acgagcctga cgcatggctg gagcacctgt
    15241 atgtaccccg aggcggagcg cggacacgtg ggctcgacct caagcggacg gagaagtatt
    15301 accagaacaa tttctacttc ctttgcaaac accgtccgcc cttctggagc gcggcgtact
    15361 acacgctggc cgtgtttctg gcggggtacg tgaggaagga agtcatacgg caaccggccc
    15421 tgctgtggca gattccgcgc atgatgtgtc ggggtatgcg ttccgccagg cgccttgcgc
    15481 gggacattcc ggccaccttt cttccggcgc cggtgcctga cgccgccccg gttacaccat
    15541 gactggagaa atcccacctg tcggtacgca tacccacgtg cagaagcttc tcctctcgtg
    15601 gggacgacag accggacacc gtgtgtgggt ggcgaaaggt gaccgggggc ggtactctga
    15661 aggccgcttt ctggctgacg ggtgtattga acgcataccc acgttcatgc cggcccgcgt
    15721 gctcagcatc ctcgagaacg tggatgtcgt ctggtttccg ccgtcgggag cggttccggt
    15781 tgcgttgttc gaggtggagc actcgacggc tattctcggg gggttgatgc gcatgaatga
    15841 cgtggtgacg acactcgtgc ctcccctgga aggatggcgc ttttttgttg ttgcgccggc
    15901 gcgcagaatt tcgagattca acggcgaact cgcccgtccg acgttccagg ccagcgggct
    15961 ggcccgggta tgccggtttc tcagctatga ccacgttgtt gagggaatgc ggaacaacct
    16021 tccgcttcgc tgacctccat ggcgctccat cctgccattc agaaccttgg ggtgctggta
    16081 aaaccggcct ccgcccgatg caatctcgcg tgcacctact gcttctacca cgaacgtgag
    16141 agcgacccct atgcgcgcgt cggttcccgt cggatgtcgg aatcggttct ggagcgtttt
    16201 ctggcggaat accttcccct tgcgggagtg caaccgacgc tcggatggca gggaggggaa
    16261 ccgctccttg ccgggctgag gttcttcgag cgcatgattg aattgcagga aaaactgcgg
    16321 gacaggtccc agacgatctc ccacgccatt cagacgaatg gaacgttgat tgacgacgac
    16381 tgggcgcgat t
//
LOCUS       DAOSOO010000055        16278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3602_length_16278_cov_5.974419, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000055 DAOSOO010000000
VERSION     DAOSOO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16278
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_16278_cov_5.974419"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1033
                     /locus_tag="PLT86_05785"
     CDS             428..1033
                     /locus_tag="PLT86_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36156.1"
                     /translation="MDPHTRDRIIPPLRRWAQPGDINAFFGLMLDNMSDLVIMAGILT
                     MAFGIPADIVLGRMIPGTAVGVLVGDLLYTWMAWRLAERTRRDDVCAMPLGLDTPSTF
                     VFCLAIIGPVFLDATARGMTPHDAGMVAWKAGMAILVLTGLVKVAVSFVGTAVRDWIP
                     RAGLLGSIAAVATVLISFLPALEVLPLRSRVFSASESSSSQ"
     gene            1099..2025
                     /locus_tag="PLT86_05790"
     CDS             1099..2025
                     /locus_tag="PLT86_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36157.1"
                     /translation="MYYVLVALGVQSGKGWTDALAGASFHVALPIPTLAFLDGLGPAL
                     RYLPIAVPMAIMTVVGGIDCTESAAAAGDEYNTRDILLVEGFATVLGGLCGGVLQSTP
                     YIGHPAYKRMGGRAAYTLATALFIGFGGVFGYLSFFVDLIPRAATAPILIFVGLEITA
                     QAFTATPSRHAPAVAIAFIPVIADLIIVELGQFLPAQNGIPPQMQGVHQTLLIMANGF
                     IFISMVWAGATAFITDRRYAAAAGLFFGAAVLTSVGIMHSPFATGEMYLPWLLSGAKA
                     HLVYTVAFAYLLLAVGLFLLRWIPEQREHSAS"
     gene            2050..3198
                     /locus_tag="PLT86_05795"
     CDS             2050..3198
                     /locus_tag="PLT86_05795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36158.1"
                     /translation="MSGRTWVVSGMAAALGGYGYAMARIVHGLTKIKPGKNDWQYSMS
                     VIVPARNEEATIRKCLEGLLEQDYPKYFYEIIVVNDRSTDSTAAIVEELRRENPRIRL
                     VNITEVPAGVAPKKNAVVTGIQHAIGRIIVTTDADCDHPKTWLTALNREFAPDVGVVV
                     GHTVYRTPRNWFEGLQAVDYLSHRIVGAGTIGEGEALNSTASNLAYRREVYDEVGGFG
                     DARGIVSGDDDLFLQRVHTMTSWRIVAPTSPESFVATQPVSSVSAFLNQRARWASKST
                     NYHAGVLPFLVATFLLLILIAFSLPFAIRRPKRWALFWALLAAKAGIDFNVMKTGSRM
                     FGQTELMKYFWLAEILMPFYVVAAVFWGLFGKFAWKGSLWGRRVAAAA"
     gene            3228..4679
                     /locus_tag="PLT86_05800"
     CDS             3228..4679
                     /locus_tag="PLT86_05800"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT36159.1"
                     /translation="MGKHSHFRQIRDCVYEAPETAQKGMRGPARIFADEQILSKAEED
                     GSLDQLLNTATLPGLVGAAMAMPDCHLGYGFPIGGVVATDARDGVVSPGGVGYDINCG
                     VRLLRTPFPAGEVREFIGALADAIFRACPSGVGSRGPRMLSAAELDDLLISGARWAVA
                     QGYGVETDLENTEDRGALDAADPDLCSHTAKKRGQEQLGTLGAGNHFIEVDYVAKLHD
                     TAAARLFGLTEGTIAIQIHCGSRGLGHQICTDSVSALQKASRSFGYTPPDKQLACAPL
                     GSEEGERYLAAMAAAANFAWANRQVLTHGVRQSFRQFLGDAVEMDDISTLYDIAHNIA
                     RMETHEVDGRNVRVCVHRKGATRALPAGHPAVPERYRAAGQPVLVPGSMGTRSYVLAG
                     NPDSLLLSLGSSSHGAGRLLSRKSSKKSEGGREVFERLSSKGIVIRTDSFRGLAEERP
                     EAYKDVDTVVSVLQRAGLARIVAELHPLAVVKG"
     gene            4734..5528
                     /gene="mazG"
                     /locus_tag="PLT86_05805"
     CDS             4734..5528
                     /gene="mazG"
                     /locus_tag="PLT86_05805"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT36160.1"
                     /translation="MTPLEELIEVMARLRAPGGCPWDREQTHETLIPYLVEETYEAVE
                     AIERKDFDELKKELGDVLLQVIFHARLAEEAGRWNIHDVAAATVAKLKHRHPHVFGNV
                     SADTPEKVLANWEQIKQKEREGSEKESVLDGVPNHLPALRKAKRVQEKVARVGFDWER
                     TEQVVEKVEEEFQEFCEACKEKDRAKTEEELGDVLFALVNLARFLDTDPEDSLRKTVD
                     KFTRRFKHIEAGLAAKGKLPQDASLAEMDALWDEAKSLEHTQQPKE"
     assembly_gap    5548..5647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5648..6358
                     /locus_tag="PLT86_05810"
     CDS             <5648..6358
                     /locus_tag="PLT86_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36161.1"
                     /translation="ELFNVAKQSDKIFMMGYNNRYRGDTQLLKKYIEAGELGDLYYGK
                     CGWIRRHGAPGPGSWFSTKSMSGGGPLIDLGVHALDLAWWLMGKPKPVSVSASVFNLL
                     MPQEWERLGRPGTMDVEDAAVAHIRFANGAAVLLEVSWLLHTPKESFYCQVMGTKGGA
                     SAEPEFRICQDKHGAEVDLTPRAPNIGGHPAEIAHFVQCIRTNTQPFSTAEDGLLVMK
                     MLDAIYRSGESGKAVTIT"
     gene            complement(6438..6947)
                     /locus_tag="PLT86_05815"
     CDS             complement(6438..6947)
                     /locus_tag="PLT86_05815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT36162.1"
                     /translation="MSDTTKRDGEQPTVNRRPVSATSALNEAYHYQLPSSFSRGMRID
                     LPCVSMIYISGTASVDENGATAHVGDFEGQVRRTFRNITELLKAEGLTWKHVVRTTCY
                     LKDIQRHYDEFNRLRTEFYKQQGLDPLPASTGIEARLCRDDLLVEIEAIAMVPGPNHQ
                     GCSEYVDKK"
     gene            complement(7102..7848)
                     /locus_tag="PLT86_05820"
     CDS             complement(7102..7848)
                     /locus_tag="PLT86_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36163.1"
                     /translation="MTGQVDTPGTEGPYPLMVLIPGGSLSRRGTSSQFPAMSRLYDPL
                     VDAALREGWAVFRYDRHETPPGSRDESQDIIDAIRAARALPDVDTRRIVLCAHASGTV
                     FLQQAYDRVEEIVGFRSLRGVILLSNRADASEAGRMAGHLLVVVGESASTDEKRAGEN
                     AVRAHRESYPRWNARYLMVPTRTWPSAIRPPPAGGAGKDCRVPAAFRRKSREQYRSFC
                     VRRPLPRSEKTSRVLPGGEFSGTLNPLYFC"
     gene            complement(7961..8854)
                     /locus_tag="PLT86_05825"
     CDS             complement(7961..8854)
                     /locus_tag="PLT86_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT36164.1"
                     /translation="MLSRERYEIKVGVVFILTVTCLIAGLLWAKRIRPRQSEMQLAAV
                     FSTVGGLGTGDEVLVNGLRLGKVEAIRLRQRDVLVTLTLDRRIPLYQGYNIRIITLNF
                     TGEMGVSIEPGAGEPLPEPYGVLRGTDPFSLEDVVQPGLETIRSVRSVAETLSVSLPP
                     LVHDARNVLSRLDNTLEEARSGVAVNKVALRRSLDDLQRVLGSANRTIASLETRLDTT
                     LTRADDAFLTIKTASDSLRRAAETIAQARGTIARLVHDPSLYEDLRRTSLHLDSAATS
                     IDSLAQDVRRNPKRYVRFSLF"
     gene            complement(8870..9349)
                     /locus_tag="PLT86_05830"
     CDS             complement(8870..9349)
                     /locus_tag="PLT86_05830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOT36165.1"
                     /translation="MILLVRVDERLIHGQVTVAWRGALDLDAFAVVDDELAGSDWEKD
                     LVLAAAPEGTVAEVVTVADAVRLWNDWNADTRRRMVLVQSFASARSICASGVDLGSLN
                     VGGLHKREGRKEFLPYVWLDSAEMETCRYLCRSGVTLEARNVPATSGVNLCARIGDS"
     gene            complement(9346..9756)
                     /locus_tag="PLT86_05835"
     CDS             complement(9346..9756)
                     /locus_tag="PLT86_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36166.1"
                     /translation="MIRAVLVCHGELSAALLTSAEEIVGHIPEVICISNRGKSPEIVR
                     QEIIHAASENAGLSGVLVMADLFGSSCWRCAMEAARVGTGVPMAVVTGVNLGMLLSFS
                     QKRDSMDFPTLASTMAEDGRRGINGPVFSSEGRT"
     gene            complement(9753..10775)
                     /gene="hprK"
                     /locus_tag="PLT86_05840"
     CDS             complement(9753..10775)
                     /gene="hprK"
                     /locus_tag="PLT86_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOT36167.1"
                     /translation="MQRRTRLATETPEQASVGITIEDLVRLHGQRLLLELLAGKAGTW
                     RRIFSAEPHRPSLPLTGFVDLYTFDRAQVLGNTELLYLRQMDPKSRYEALYIIFQFEL
                     PCVIITNAYEVFPELIQLAEEREIPLLRTSLSTARFVHHYVAALDFDFAPQTTVHGTL
                     VDVYGVGLLLTGRSGIGKSEIALDLVERGHRLVADDVVHLRRQRRVLMAEANRLLGFN
                     IEVRGIGILDVERLFGIRAIRARKRVEVQVLLTPWDGAEDYERTGLDDERSAILGVEI
                     PLVRLPIFPGKNVTVICETIAMNHLLKVRGYNAAEVLSNRLTSELNKASETVPRPSNL
                     SDLDAT"
     gene            complement(10747..11070)
                     /gene="raiA"
                     /locus_tag="PLT86_05845"
     CDS             complement(10747..11070)
                     /gene="raiA"
                     /locus_tag="PLT86_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT36168.1"
                     /translation="MQVFITARHCEISQTFRDRITSEVEKLTKIFERINRVEIIVSED
                     AGVNQVEINFTVNQQAFNAKGQGASHQLAFDIALEKAERQLRKFKGKLVGRRAIVAKE
                     DQTGN"
     gene            complement(11221..12582)
                     /gene="glmM"
                     /locus_tag="PLT86_05850"
     CDS             complement(11221..12582)
                     /gene="glmM"
                     /locus_tag="PLT86_05850"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT36169.1"
                     /translation="MGELIISISGVRGIVGEMLTPETALGMTAAFAEVAPEGEILVAS
                     DGRTSGDSLRAACLAALSAKGRTAVDLGVATTPTTEMAVILRKAAGAIIVTASHNPVE
                     WNGLKFLDQEGVFLRQGQLDRLVSIWKRSVPEWKRWEFVQPVLNWDGAADAHVERILA
                     LDVVNAEACRQARLPVVLDTICASGGRIAPKLLRALGSPFVHVNAEITGKFTRKPEPV
                     PENIRDVGEAVRANHAALGMVLDPDGDRLALLDETGEPIGEEYTLALSIYAVRRRART
                     RAPVVVNASTSRMVDDVGAMFGFPVLRTAVGEINVVEGMVKHGADLGGEGNGGVIYGG
                     LHYGRDGLLGMAIILSLLAEEKCRLSDLVRKLPRYVISKQTATVPPEKRAAVLHRVRS
                     LAAEMGAELDTCDGVKLVWADRWLHVRPSNTEPIIRIFAEAPTEQDARELCARAQEIA
                     ARE"
     gene            complement(12677..15235)
                     /locus_tag="PLT86_05855"
     CDS             complement(12677..15235)
                     /locus_tag="PLT86_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36170.1"
                     /translation="MAKRPASPPRAAKTPHFDQKPPLSRPMVHAAFAGLAVLLVLIYY
                     GSLILKSSPPQAPDTVASDAISKMSVLWRDQHNGEMPLWTPAVFSGMPSYGAMIYTHE
                     NPISFVLGKATFGNFGIAMALYTLIGGVCVYALLTRYGRSPLASFFAAAVYMFTPYIP
                     GLINAGHNNKILAAAIIPALLLVTDGLLRERSLKAFGWFVLVAAWQLWVRHPQVTYYG
                     IMLVACIVLADVFVQQGALLARARRLAEDAVLLGAGLVFALGIVADPYLPMLEFTPES
                     VRGGAPSAASELAVEAGQASDRGWEFATQWSMHPKELVSFVVPSFYGLWNDPRYNPQT
                     DIEAHTYWGYMPFTQSTHYLGLIPLILALLVRPNRTALVWGCLVFSGMALLIGLGHWF
                     PVLYWPAYKLLPYFAKFRVPSMIYMLLPLSIGIVGAWALDQFMERETVAGRVRSARRF
                     AREEKIALAVSVAMVVLALAVMVLHRSPGWTLKAGEENYPAQIQDALAKVRSGLLARD
                     VLIALVLAALTAGGLILVSGRRMSGTIFAAILTVATIADLWRLDFTFLDIRRPTFAEA
                     PIQQPAEVGIIRNDLQAPIPDSVRLWHHIATGDTLFRIAPVAGLDQAGAMNIESTNEY
                     GLWSLPSVSGYHAAKLRIYDDLTISGGLSRRQVLNMLNARYVIAPPGLPDSTLVPVGG
                     GERTVYRNTTALPRAWWVSSVRAVNDRKAALSSVLGPDFDPAEEAIVLGPAPQILPDS
                     TLAPPRVSLWDFHRIEIETNNSRPAFLVLSEIFYPRGWRATADGKPVEIVQTNFVLRG
                     LEVPAGTKKIVLTFDSAAYRWGRRLSFTLFPLFLLLVAEETWRAGRKKKGNPTG"
     gene            15437..>16278
                     /locus_tag="PLT86_05860"
     CDS             15437..>16278
                     /locus_tag="PLT86_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36171.1"
                     /translation="MEGSRQRVRDVIAGKKPDRIPLFELIRNDAVIEHFTGKKLTPEN
                     GQELVYETFPKAVDATRPALKTPNHPREETLPDGRRQVVQRWTIWTEHVKYPSGKAYR
                     DAEMAQPIEQEWTEEDDAGIQRHLEHHRDIQRHLGDDFFLFYGGPSVGFMGTFGEIGL
                     EQYCYFAADYPEIVDHRLERNTVRSIQWIEHLPANHGIEAVFMGDDIAFKGGPMFSPA
                     WFAKHYMPRMKRIVEAYHRRGIKVNFHSDGNLMPIMDGLVDAGIDLLNPIETAAGMDI
                     AEMHR"
BASE COUNT         3350 a         4940 c         4733 g         3155 t
ORIGIN      
        1 cgccttcgcg tgcgccattt cttcgcctcc cgaaaaaacc tggtgcaaat ttgcagcttc
       61 ttgacgaaaa ggcttgacac gaagacgacg ttttgcccca gccggtgtgt ggggaggtat
      121 ttcgtgcgcg gcaaaaatcg ggcacgacgg agcgtgcccc tccaaccggg cgtgtagggg
      181 tcacgatgtg ggtgtagggg ccgggtcgcc cggcccacgg aggcaaaaga acaaatgccg
      241 gcggcgggcc tacgttttgc gcggttctcg ttccgtttgt ggcggaagat gggcacgacg
      301 gcggctcgcc acgccatccc ccgtggtgtt tgcatgacga actcgctatt ttgaccattc
      361 cccggacgat tgatcgaggc ggaattcggg gcatccgatc acacccacgc cacaggagtc
      421 tgttcccgtg gacccacata ctcgcgacag aatcatcccg ccgcttcgcc gctgggcgca
      481 gccgggcgac atcaacgcgt ttttcggact gatgctcgac aacatgagcg atctcgtcat
      541 tatggcggga atcctcacga tggcgttcgg aatcccggcg gacatcgtgc tcggaagaat
      601 gatccccggc acggcggtcg gtgtgctggt gggcgacctt ctctacacct ggatggcatg
      661 gcggcttgcg gaacgcaccc ggcgggacga tgtgtgcgcc atgcccctcg gtctcgatac
      721 gccttcgacg ttcgtgttct gcctcgcgat catcggcccg gtgttcctcg acgccaccgc
      781 gagaggaatg acgccccacg acgcgggaat ggtcgcatgg aaagccggga tggcgattct
      841 cgtgctgacg ggtctcgtaa aggttgcggt atcattcgtc ggaacagcgg tgcgagactg
      901 gattccccgg gcgggattgc tgggttctat cgcggcggtt gccacggtac tgatctcgtt
      961 cctccccgcg ctggaggtgt tgcctctccg atcgcgggtt ttctcagcct cggaatcatc
     1021 ctcgtcgcag taatcggcgg gagggcactt cccttccggt tcccggctgc gttcggcgca
     1081 gtgcttctgg gtgggattgt gtactacgtc ctggttgcgc taggcgttca gagcggcaaa
     1141 gggtggactg acgcgctggc cggggcctcg ttccatgtgg cactgccgat ccccacactg
     1201 gcgtttctgg acggactggg cccggcattg cggtatctgc caatcgccgt acccatggcc
     1261 atcatgacgg tcgtcggcgg aatcgattgc acggaaagcg cagcggcggc cggggatgag
     1321 tacaacacgc gcgacatttt gctcgtggaa ggattcgcca ccgtattggg cgggctttgc
     1381 gggggcgttc tgcaatccac accgtatatc ggccatcccg cctacaagag gatgggggga
     1441 agagcagcgt acacactggc aacggctctg ttcatcggct ttggtggggt attcggatat
     1501 ctgagttttt tcgtcgacct gatcccgaga gcagcgactg ccccgatcct gatttttgtc
     1561 ggacttgaga tcaccgcaca ggcattcacc gcgacgccgt cacgccacgc accagcagtg
     1621 gcaatcgcct tcattccggt aatcgcggac ctgatcatcg tcgaactggg ccagtttttg
     1681 ccggcacaga acggcattcc tccgcagatg cagggggtgc atcaaacgct tcttatcatg
     1741 gcaaacgggt ttatctttat ttcaatggtc tgggcgggtg ccacggcatt catcacggac
     1801 cgccggtatg cagccgcagc cggcctgttt tttggcgcgg ccgtgcttac gagcgtcgga
     1861 atcatgcatt cgccattcgc gaccggcgag atgtacctcc cctggcttct gtccggggcg
     1921 aaagcgcatc tggtgtatac ggtggcgttc gcgtatctgc ttctcgcggt aggactgttt
     1981 ctgctgcggt ggattccaga gcagcgcgag cactctgctt cctgagcacg caaaggggag
     2041 aatcagttca tgtcaggacg cacatgggta gtaagcggaa tggccgcggc actgggcgga
     2101 tacgggtacg ccatggcacg tatcgtccac ggtctgacga agatcaagcc cggcaagaac
     2161 gactggcagt acagcatgag cgtcatcgtt ccagcgagga acgaagaagc cacaatccgg
     2221 aaatgcctgg aaggcctgct tgagcaggat tacccgaagt atttctatga aatcatcgtg
     2281 gtaaacgatc gttcgactga cagtaccgcg gcgatcgtgg aggaactgcg ccgcgagaac
     2341 ccgcgaatcc ggctcgtgaa catcaccgaa gtaccggcgg gcgttgcacc caaaaagaac
     2401 gcagtcgtca ccggaatcca gcatgcaatc gggcgcatca tcgttacaac ggacgccgac
     2461 tgcgatcacc caaaaacctg gttgacggca ttgaacaggg aatttgcccc tgacgtaggt
     2521 gtggttgtcg gacacaccgt gtatcgaact cctcgcaact ggtttgaagg gctccaggcg
     2581 gtggattacc tctcgcacag gatcgtcggc gcgggaacta tcggcgaagg cgaggccctg
     2641 aacagcaccg caagcaatct cgcgtaccgc agggaggtgt acgatgaggt tggcgggttt
     2701 ggagacgcac gcggtatcgt tagcggcgac gacgatctct tcctccagcg cgtgcacacg
     2761 atgacttcat ggcgcatcgt cgcaccgaca tctcccgaat cttttgttgc cacacaaccg
     2821 gtttccagtg tgtctgcctt cctgaaccag cgtgcccgtt gggcatccaa atccacaaat
     2881 taccatgccg gagtgcttcc attcctcgtc gcaacctttc tgttactgat tcttatcgcg
     2941 ttctcgttgc catttgcgat tcgccgcccg aaacggtggg cgctcttctg ggcgcttctt
     3001 gccgcaaagg ccggcatcga tttcaatgtc atgaagaccg gttcccgcat gttcgggcag
     3061 acggaattga tgaagtactt ctggctcgcg gaaatcctca tgccgttcta tgtagttgcg
     3121 gcggttttct ggggcttgtt cgggaaattc gcgtggaagg gttccttgtg ggggcgtcgc
     3181 gtggcagcag ccgcgtgaat cacgccactg tgcgcggttg agcttccatg ggaaaacact
     3241 ctcacttccg gcagatccgc gattgcgtgt atgaagcacc ggagacggcg cagaaaggta
     3301 tgcgcggtcc cgcgcggatt ttcgcggacg agcagatcct gagcaaagcg gaggaggatg
     3361 gttctctcga tcagttgctg aacaccgcaa cgttgccggg tcttgtcggc gccgccatgg
     3421 ccatgcccga ttgccacctc gggtacgggt ttcccatcgg cggagtcgtg gcaacggacg
     3481 cgcgcgacgg cgtggtatcc cccggcggag tgggatacga catcaactgc ggggtccgcc
     3541 tcctgcgcac gccatttcct gctggtgagg tgagggagtt cattggcgca cttgcggacg
     3601 ccatatttcg agcgtgtccc agcggagtcg gctcgcgagg tccacggatg ctatcggcgg
     3661 cggaactgga cgaccttctg atatcggggg cgcggtgggc ggtcgcgcag ggatacggcg
     3721 tggaaaccga tctggagaac acggaagacc gaggcgcgct tgatgctgcg gatcccgacc
     3781 tctgcagcca cacggcgaaa aagcggggcc aggaacagct cggaacactc ggtgccggca
     3841 accattttat tgaggtcgat tacgttgcga aactccatga taccgcagca gcacgcctgt
     3901 tcgggctgac ggaaggcacc atcgcgatcc agatccattg cggatcgcga ggcctggggc
     3961 accagatctg caccgattcc gtgagcgcgt tgcagaaagc ctcccggagc ttcggataca
     4021 ctcccccgga caaacagctc gcgtgcgcgc ctctgggaag cgaggaaggg gaacgttacc
     4081 ttgccgcgat ggcggccgcg gcaaatttcg cgtgggccaa caggcaggtt cttacccacg
     4141 gagtacgcca gtctttccgt caatttctgg gtgacgcggt agaaatggac gatatcagca
     4201 ccctgtacga catcgcgcac aatatcgcga ggatggaaac acacgaagtg gacggccgga
     4261 acgttcgagt gtgcgtgcac cgcaaaggtg cgacgcgagc cttgccggcg ggtcatccgg
     4321 ctgttccgga gcgatatcgg gcggccgggc agccggtgct cgtcccgggt tccatgggaa
     4381 cgcgttccta cgtgctcgcc ggaaatccag actcccttct actgagtctc ggttcaagct
     4441 cgcacggcgc gggaaggctg ctctcccgaa aatcctcgaa aaaaagtgag ggcgggaggg
     4501 aggtgttcga aaggctctcg tcgaaaggca tagtcatcag aacggattcg ttccgcggac
     4561 ttgcggagga acgacccgaa gcctacaaag acgttgacac ggtggtctcc gtactgcagc
     4621 gggcagggct cgcccgcatt gttgccgagc ttcatcctct tgccgtggtc aaaggataac
     4681 cgacccttgt ccttcgcgcg ttgcattccc ctcaacctca ttcagggtac ctcatgacac
     4741 ctcttgaaga actgatcgaa gtgatggctc gcttgcgggc accgggaggg tgcccctggg
     4801 atcgcgaaca aacgcacgaa actctcattc cttacctcgt ggaggaaacg tacgaggcgg
     4861 tcgaggcaat tgaacgcaag gatttcgatg agctgaagaa ggaacttggc gacgtgctgc
     4921 tgcaggtcat cttccatgcg cggcttgcag aagaagcggg ccggtggaat atccatgatg
     4981 tggcagcggc gaccgtggcg aaattgaaac atcggcatcc ccacgtgttc gggaatgtat
     5041 ccgcggacac gccggaaaag gtcctcgcca actgggaaca gatcaagcag aaggaacgcg
     5101 aaggatcaga aaaggaatcc gtgcttgatg gagttcccaa ccacctcccc gcactgagga
     5161 aagcaaaacg ggtgcaggaa aaggtcgccc gggtgggatt cgattgggag cgcacggaac
     5221 aggtggtgga aaaagtcgag gaggaattcc aggagttctg cgaagcgtgc aaagagaaag
     5281 atcgggcgaa aaccgaagag gaactgggtg acgtcctctt cgcgcttgtg aatctggctc
     5341 ggttcctcga caccgacccc gaagattctc tccggaaaac ggtggacaaa ttcacacgcc
     5401 ggttcaaaca catagaagcg gggcttgcgg ccaagggcaa actgccacag gatgcaagcc
     5461 tggcggagat ggatgcactc tgggacgagg cgaaatctct cgaacacaca caacagccca
     5521 aggagtgatc atggcagtca gaccacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnngag ctgttcaacg tcgcaaagca atcagacaag attttcatga tgggatacaa
     5701 caaccgctac cggggagata ctcaactcct gaaaaagtac atcgaggccg gggaactggg
     5761 tgacctctat tacggaaaat gcggctggat tcggcggcac ggggcacccg gacctggttc
     5821 atggttcagc acgaaaagta tgtcgggcgg cgggccgctg atcgatttag gagtccacgc
     5881 actcgacctt gcgtggtggt tgatgggcaa accgaagccg gtttcggtga gcgcatcggt
     5941 attcaatctg ctcatgccac aggaatggga acgactcgga cgtcccggaa caatggacgt
     6001 tgaggacgcc gcagtggctc acatcaggtt tgcgaatgga gcggcggtgc ttctggaggt
     6061 gagctggctt ctgcacactc ctaaggaaag cttctactgc caggttatgg gaacaaaggg
     6121 tggcgcttcc gcggagcccg agttccgcat ctgccaggac aagcacggcg cagaagtgga
     6181 tctcacaccc cgcgcaccaa acatcggcgg gcaccccgca gaaatcgcgc atttcgtgca
     6241 gtgtatccgc acgaacaccc aacccttttc cactgccgaa gacggccttc ttgtcatgaa
     6301 gatgctggac gcgatatacc gctccggcga aagcggcaag gccgttacta ttacctgacg
     6361 cacggagatg cggcgcgtga agggcaacaa aaagggcggc tggtgagccg ccctttcgcg
     6421 tttctgtttc ttcacgccta tttcttgtcc acgtactccg agcatccctg gtgattcggg
     6481 cccggcacca ttgcaatggc ctcgatttcg acaagcaggt catcacggca cagacgtgct
     6541 tctatcccgg tgctggcggg caacgggtcg agcccctgct gcttgtaaaa ctcggttctg
     6601 agccggttga attcgtcgta atgccgctgg atatccttga ggtaacaggt cgttcttacg
     6661 acatgtttcc atgtcaggcc ttccgctttg aggagttcgg tgatattcct gaaggttcgc
     6721 cgcacctgtc cttcgaaatc ccccacatgt gccgtggcac cgttttcgtc cacgctggca
     6781 gttccgctga tgtagatcat agacacgcag ggaaggtcaa ttcgcatacc tcgcgaaaaa
     6841 gacgacggaa gctggtaatg gtaggcttcg ttcaacgcag atgtcgcaga aacaggtcgc
     6901 cggttgaccg tgggctgttc cccgtcgcgt tttgtcgtat cgctcatgga tactcctctg
     6961 gcgtagcgtg cggaaacacc gcagaaaggt tcatccggag cggattgtgc caaggcaacc
     7021 aaatgcggcg ggaacgaaga aactcgtcac accgacaaaa agaccacgtg gcgcgaatcc
     7081 ccgtttgcgt ctcagcggcg ttcaacaaaa atacaacgga ttaagggtac ccgagaattc
     7141 tcccccgggc aacacccgtg acgtcttctc agaacgtggc agcggacgcc gcacgcagaa
     7201 gctccgatat tgctctcgag atttccggcg aaaagcggca ggaacccggc aatccttgcc
     7261 agccccgcca gccggaggtg gtcgtatcgc agagggccac gtccgcgttg gaaccatcag
     7321 atagcgcgcg ttccatcgag ggtacgactc gcggtgggct cgaacggcgt tctcacctgc
     7381 gcgtttctcg tccgtcgagg ctgattcccc gacgacgacc agaaggtggc ccgccatgcg
     7441 tcccgcttcc gaagcatccg cccggttcga aagcagaatc accccgcgca acgaccggaa
     7501 tccgacgatt tcctccaccc tgtcgtaggc ctgttgcagg aacacggtgc cggaggcatg
     7561 tgcacacaag acaatacgcc ttgtatccac gtcgggaagg gcccttgccg ccctgattgc
     7621 atctatgatg tcctgcgact catctctgct gccgggtggt gtttcgtgcc gatcgtaccg
     7681 aaaaaccgcc cacccttctc tgagtgcggc gtccaccagc gggtcgtaca accgcgacat
     7741 cgcgggaaac tggcttgatg tgccacgccg tgagagactg cctccgggaa tgagaaccat
     7801 gagagggtag ggaccttccg tgccgggtgt atcaacttgc ccggtcaccc ccgatcccgt
     7861 tccgaagcgc acgtctcgcg gggcgggacg ggcagtcgcc tgagcccccg gcaaaaccat
     7921 gacggccagg catactagga ggaaccgttg acttcggaga tcagaacagg ctgaaacgca
     7981 cataccgttt cgggtttctg cgaacgtcct gcgcgaggga gtcaatgctt gtcgcagcag
     8041 aatcgaggtg aagagatgtg cgacggaggt cctcgtacaa cgatgggtca tgcacgagcc
     8101 tggcgatagt cccccttgcc tgagcgatcg tttccgctgc cctccgcaga ctgtcggaag
     8161 cagtcttgat ggtcaaaaac gcgtcgtctg cccgcgtcag ggtagtgtcc aacctggttt
     8221 cgagcgaagc gatggtacga tttgcggatc cgagcacgcg ctgaaggtcg tcaaggcttc
     8281 tccgaagggc cactttgttc accgctactc cggaccgtgc ttcttcgagc gtgttatcca
     8341 gacggctgag gacgttcctt gcatcatgaa caagcggcgg cagtgaaaca ctcagcgttt
     8401 cagcgaccga ccgtaccgag cggattgtct caaggcccgg ttgcaccacg tcttccaggc
     8461 tgaaagggtc tgttccccgg agcactccgt acggctcggg gagaggctca ccagcccctg
     8521 gctcgattga gacgcccatt tctccggtaa aattcaacgt gatgatgcgg atgttgtagc
     8581 cttgatacag cggaattctg cggtccagag tgagcgttac gagcacatcg cgctggcgca
     8641 ggcgtatggc ctccaccttc cccaggcgca acccgttcac gagcacctca tcgccggtac
     8701 cgaggcctcc gacagtcgag aacactgccg caagctgcat ctcggactgc ctgggccgga
     8761 tccgcttcgc ccagagcaag ccagcaatga ggcaggtgac cgtcagaata aaaacgacgc
     8821 ctactttgat ctcgtaccgc tcacgcgaga gcatccacgc acctttcgct caggaatccc
     8881 cgattcgcgc acacaggttg acacccgacg tggcgggaac gttgcgagct tccagcgtaa
     8941 cccccgaacg gcaaaggtac cggcaggttt ccatttcggc actatcaagc cagacgtacg
     9001 gcaggaattc cttcctccct tcgcgcttgt ggagccctcc cacgttcagc gatccgaggt
     9061 ccacacccga ggcacagatt gaccgggcgc tggcaaacga ctgcacaagg accattcgac
     9121 gccgggtatc ggcgttccaa tcattccaca ggcgcaccgc gtccgccaca gtgacgactt
     9181 cggccacagt tccttccggc gccgccgcaa gaaccaggtc cttctcccaa tcacttccgg
     9241 cgagctcatc gtccacgacc gcaaatgcat cgagatcgag ggcaccccgc cacgccaccg
     9301 taacctgccc gtggatcagc cgttcgtcga cacgcacgag cagaatcacg ttctgccctc
     9361 cgacgaaaac accgggccgt tgatgccgcg tctgccgtcc tctgccatcg tcgaggccag
     9421 cgtcggaaaa tccatgctgt cgcgtttctg gctgaaggaa agaagcattc cgaggttgac
     9481 tcccgtcaca acggccatcg gcacgcctgt tccgacacga gcggcttcca ttgcgcaacg
     9541 ccagcaactc gacccgaaca ggtccgccat cacaagaact ccggacaaac cggcgttttc
     9601 actcgctgcg tggattatct cctgccgcac gatttccggg gatttccccc ggttcgatat
     9661 gcagatgact tcggggatat gaccgacaat ttcctccgca gacgtgagaa gtgctgcgct
     9721 caactccccg tgacacacaa gcaccgcgcg tatcatgtgg cgtccagatc gctcaggttg
     9781 gatgggcgcg gcaccgtttc cgacgccttg ttcagttcac tcgtgagccg gttcgaaaga
     9841 acctcagcgg cattgtagcc gcgcactttg aggagatggt tcatcgcgat cgtctcgcag
     9901 atcaccgtca cgttcttgcc ggggaaaatc ggcagcctca ccaggggtat ctcaacaccc
     9961 agaatcgcgc ttcgttcgtc atccagaccg gttcgctcgt agtcttcagc cccgtcccat
    10021 ggggtcagaa gcacctgcac ctccactcgt ttccgcgccc ggattgcacg gatgccgaac
    10081 agcctttcca catccagaat cccgattccc cgcacctcaa tgttgaagcc aagcagccgg
    10141 ttcgcttctg ccatgagcac ccggcgctgt ctccggagat gcaccacatc gtcggcaaca
    10201 agccggtgcc cacgttcgac gagatcaagg gcgatttcgc tcttcccgat acccgaacgg
    10261 ccggtcagga gaagccccac gccgtacacg tccaccagtg tgccgtgcac ggttgtctgc
    10321 ggggcgaaat cgaagtccag cgcggcgacg tagtgatgaa cgaatcgcgc tgtactgagc
    10381 gacgtgcgga ggagcgggat ctcccgctcc tccgcaagct ggatcagctc cgggaacacc
    10441 tcgtacgcgt tcgtgatgat cacgcacggc aactcgaact ggaagatgat atacagcgcc
    10501 tcgtatctgc ttttgggatc catctgccgc agatacagaa gctccgtatt gccgagaacc
    10561 tgggcacggt caaaggtgta caggtccaca aaacccgtga gcggaagcga cggccgatgt
    10621 ggttccgcgg aaaatatccg ccgccaggtg ccagccttgc ctgccagtag ctccaacagg
    10681 agtcgttgac cgtgcaatcg aacaagatcc tcaatcgtga tgccgacaga ggcttgttcg
    10741 ggagtttcag ttgccagtct ggtcctcctt tgcaacaatc gcgcgcctgc ccactagctt
    10801 gcccttgaat ttccgcaact ggcgctctgc cttttcgagc gcaatatcga acgcgagctg
    10861 atgagaagct ccctgccctt tcgcattgaa agcctgttga ttcaccgtga aatttatctc
    10921 aacctgattt acgcctgcat cctctgatac gatgatttcc actcgattga tccgttcaaa
    10981 aatcttcgtc aacttttcga cttcgctggt gattcggtct ctgaatgtct ggctgatctc
    11041 gcaatgccgg gcagtgatga agacttgcat ggcttgtctc tcctgctgcg ggtggacttc
    11101 tcctcgggat tccccgtgag cttcacgggg cattcctgaa cgggaagtta tccttcccgg
    11161 actcattagt caactatgcg cgggcacgct tttcggttcc cccgacgccg ccacacctcc
    11221 ttactctctc gcggcgattt cctgcgcacg cgcgcagagc tcacgcgcat cttgctcggt
    11281 aggtgcctcc gcaaaaatcc gtatgatcgg ctcggtgttc gacgggcgta catgcagcca
    11341 ccggtctgcc cacacgagtt tcactccgtc gcacgtgtcg agctccgcgc ccatttccgc
    11401 ggcaagggac cgaacacgat gaagtactgc ggcacgtttt tccggcggta cggtggcggt
    11461 ttgtttggag atcacgtatc gaggtagttt tcgcaccaga tctgaaagcc ggcatttctc
    11521 ttccgccaga agcgagagaa tgattgccat cccgagcagg ccgtcacgcc cgtagtgcaa
    11581 gccgccgtag atcactccgc cattcccctc gccaccaagg tcggctccgt gcttcaccat
    11641 cccttcaacc acatttatct cgccaacagc ggttcggagc acgggaaacc cgaacatggc
    11701 accaacgtca tccaccattc gactggtgga cgcgtttacc acgacgggcg cgcgggtgcg
    11761 tgcgcggcgc cgaaccgcgt agatgctgag cgcgagcgta tactcctctc ctatcggctc
    11821 tcccgtttcg tcaagaagcg ccaaccgatc cccgtcagga tcgagtacca taccaagtgc
    11881 cgcgtggttg gcgcgcacgg cttctcccac atcccgtatg ttctcaggaa caggttccgg
    11941 tttcctggtg aatttcccgg tgatttccgc gttcacgtga acaaacggcg atccgagggc
    12001 ccgcaacagc tttggcgcaa tccgtcctcc cgatgcacag atcgtatcaa gtaccaccgg
    12061 aagcctcgcc tgcctgcacg cctccgcgtt cacaacgtcc agcgcgagga ttcgttccac
    12121 atgggcgtcc gccgctccgt cccagttcag aaccggttgc acgaactccc atctcttcca
    12181 ctcaggaaca gagcgtttcc agatggaaac caggcggtcc agttgcccct gccggaggaa
    12241 cacaccttcc tggtccaaga acttgagccc gttccattca accgggttat ggcttgccgt
    12301 aactatgatt gcgccagccg ccttgcgaag gatcactgcc atttctgtcg tcggcgttgt
    12361 ggcaaccccg agatcgaccg cggtgcgtcc tttcgcgctc aacgccgcga ggcaggcagc
    12421 ccgaaggcta tcccccgagg tgcggccatc cgatgccacg aggatttctc cttcgggagc
    12481 cacctccgca aacgccgcag tcattccgag tgctgtctcc ggggtaagca tttcgcccac
    12541 aatgccccgc acgccggaaa tgcttattat caactccccc atttgttact ccccgtttcc
    12601 cacaccgttt gagccctttc cggttggtcc aacgcccgtc cgcacaaccg ctcccgcgcg
    12661 attcgtctgg ccggaatcac cccgtcgggt tccccttctt tttccgcccg gcccgccagg
    12721 tttcctctgc gaccagcaga aggaagagcg ggaaaagcgt gaaggagagc cttcttcccc
    12781 accggtatgc ggcggagtcg aatgtcagta cgattttttt cgtacccgcg ggcacttcca
    12841 gtccccggag cacaaagttg gtctgaacaa tttccacggg tttgccatcc gcagttgcgc
    12901 gccatccccg cgggtaaaag atctcgctca gcaccagaaa tgcaggtcgg gaattgttgg
    12961 tttcgatctc aatgcggtgg aaatcccaga ggctcactcg gggaggagcc agggtgctgt
    13021 ctggcaatat ctgcggcgcc ggccccagca cgatcgcctc ctccgcgggg tcaaaatccg
    13081 gcccgagaac cgaggacagc gctgctttcc ggtcattgac cgcgcgcacc gagctcaccc
    13141 accatgcccg gggcaacgcg gtcgtattcc ggtaaacggt tctttccccg ccgccgacgg
    13201 gaaccagagt cgaatccggc agaccgggtg gtgcaatcac gtaacgggcg ttcagcatgt
    13261 tgagcacctg acgccgcgaa aggcccccgg aaatagtcaa atcgtcgtat atacggagct
    13321 tggccgcatg gtatccgctg acgctgggca gactccacag gccgtactcg ttcgtgctct
    13381 caatgttcat ggcgcccgcc tgatcaagcc cggccaccgg agcaattcgg aagagggtgt
    13441 ctcccgtggc gatatggtgc cacagacgca ccgagtctgg aatcggcgct tgcaggtcgt
    13501 tccggatgat gcctacttca gccggttgct gaatcggcgc ttcggcgaag gtcgggcggc
    13561 ggatatccag gaacgtgaaa tcgaggcgcc agaggtcggc gatggtggca acagtaagaa
    13621 tcgccgcgaa gatcgttccg ctcattctcc gcccgctgac aagaatgagg cctccggcag
    13681 tcagcgcagc cagaaccagg gcgatcagca cgtccctcgc cagcagaccc gaccggacct
    13741 ttgccagcgc atcctgaatc tgagcggggt aattctcctc tcccgctttc aacgtccagc
    13801 ccggcgatct gtgcagaacc atcacggcca gagccagcac caccatcgcg acgcttaccg
    13861 cgagcgcgat cttctcctcc cgcgcgaatc gcctcgcaga acgcacgcgg cccgccacgg
    13921 tttcgcgttc catgaattga tcaagtgccc atgcgcccac aatgcctatg gagagaggca
    13981 ggagcatgta gatcatgctg ggaacgcgga acttcgcgaa atacggcagg agtttgtatg
    14041 ccggccagta cagaacggga aaccagtgcc ccagaccgat cagaagcgcc attccgctga
    14101 acacgagaca tccccagacc agagccgttc ggttcggccg caccagaagc gccagtatga
    14161 gcggaataag accgagatag tgcgtggact gagtaaacgg catgtatccc cagtacgtgt
    14221 gcgcctcgat atccgtctgc ggattgtacc gcggatcgtt ccacagcccg tagaacgatg
    14281 gaaccacgaa acttaccagc tccttcgggt gcatggacca ctgcgtagcg aactcccagc
    14341 cacggtcgct ggcctgcccc gcctcaacag caagttctga ggcggcgctc ggagccccgc
    14401 cgcggaccga ttccggagta aactcgagca tcggcaggta cggatccgcg acaatgccca
    14461 gcgcgaagac cagtccggcc ccgagaagca cggcatcttc cgcaaggcgc cttgcccgcg
    14521 cgagaagcgc accctgctga acaaacacat ccgccagcac gatgcacgca accagcatga
    14581 tcccgtaata cgtaacctgc gggtgccgca cccagagctg ccacgccgcc accagcacaa
    14641 accacccgaa agccttcagc gatcgttcac gaagcagccc gtcggtgaca agaagcagtg
    14701 cgggaattat cgcggcggcg aggatcttgt tgttgtgacc cgcgttgatg agcccgggaa
    14761 tgtacggggt gaacatgtac acggccgcag cgaagaagct ggccagaggc gatctcccgt
    14821 accgcgtgag caacgcgtac acacatacgc caccgatgag cgtgtagagt gccatcgcga
    14881 tcccgaagtt cccaaaggtc gctttcccca gaacaaagct gatcgggttt tcgtgggtgt
    14941 atatcatggc tccatacgaa ggcatccccg agaacaccgc cggtgtccag agcggcattt
    15001 caccgttgtg ctgatcccgc cagagcacac tcatcttcga gatcgcgtcc gacgcgaccg
    15061 tatccggcgc ctgcgggggt gacgacttga gaatcaggct tccgtagtag atcagtacga
    15121 gcagcaccgc cagccccgcg aatgccgcgt gcaccatcgg gcgggaaagc gggggtttct
    15181 ggtcgaaatg cggcgttttt gctgctctgg gaggactggc ggggcgtttt gccatgcgtt
    15241 gacacctcat cgagcggaga acgaaccgga accaccggaa cggggtgcgt agtacaccga
    15301 atgtaccgtt cccgatgtgc tccgggaaag cgccttgtca gaacggcctt gcggcgggcg
    15361 ttcccggttg ccccctgaac agggattgtg cactctctga aatggacacc taggcttgca
    15421 gcggaaggag cgcagagtgg aaggatcacg acaacgcgtg cgcgacgtca tcgccggcaa
    15481 aaaaccagac cgcatcccgt tattcgaact gattcggaac gacgccgtaa tcgagcactt
    15541 cacgggaaag aagctgacgc cggagaacgg gcaggagctg gtctacgaaa ccttcccgaa
    15601 agccgtcgac gccacccgcc ccgccctgaa gaccccaaac cacccgcgcg aagagacact
    15661 cccggacggg cggcgccagg ttgttcagcg gtggacaatc tggaccgagc atgtcaaata
    15721 cccctctggc aaggcatatc gggatgccga gatggcccag cccatcgaac aggaatggac
    15781 cgaggaagac gacgcgggaa tccagcgcca tctcgaacac caccgcgata ttcagcgtca
    15841 ccttggcgac gacttttttc tattctacgg cggcccgtcg gtcggtttca tgggcacatt
    15901 cggcgaaatc ggcctggagc aatactgcta cttcgccgcc gactaccccg agatcgtcga
    15961 ccaccggttg gagcggaata ccgtccggtc aatccaatgg atcgaacacc tccccgccaa
    16021 ccatggcata gaagccgtgt tcatgggaga cgatatcgca ttcaaaggcg gaccgatgtt
    16081 cagcccggca tggttcgcaa aacactacat gccgcggatg aagcggatcg tggaggcgta
    16141 ccacaggcgg gggatcaagg tcaatttcca ctcggacggc aacctgatgc cgatcatgga
    16201 tgggctagtg gatgcaggca ttgatctgct caaccccatc gaaaccgccg ccggcatgga
    16261 catcgccgag atgcaccg
//
LOCUS       DAOSOO010000056        16202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3621_length_16202_cov_5.665511, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000056 DAOSOO010000000
VERSION     DAOSOO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_16202_cov_5.665511"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1347
                     /locus_tag="PLT86_05865"
     CDS             121..1347
                     /locus_tag="PLT86_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT36172.1"
                     /translation="MLFLAASWILPSTARAKSLGEIVLSVPVDTVVEAGQQFLLPVRI
                     SGVTVLDSVTSFNFGVRVPLGIMLVDTAAVKGPALPSGAGFSSAKRDSVIVVGGYWTL
                     PLANNGTLFFLRCTPKANIPDNTSGEVRLERLSQNGTLLYYLNERNKPPCTAQNGSVR
                     VRNLNMVPVRIAVSSDSSVDAGSPCSIQVQGSGLISFSGTTTLQFALTVPPGLYQSVS
                     VQGGLLPADAVTLADAGRGLFRVSGSVANPAGGTGTLFSVQGLTKQDIPDSTVFAIAI
                     ERLSDGGSPLPYVRKGDQTPDCTVSNGAVTVRRPVSVAHGGASVPAPSELTAYPNPFN
                     AVITFRVHGPLSDGPIDATLYTVLGTVVRRWSQSTPEWTWDGRDASGVPSASGVYVVV
                     VRNGEKTWQKRVTLLR"
     gene            1555..6849
                     /locus_tag="PLT86_05870"
     CDS             1555..6849
                     /locus_tag="PLT86_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT36173.1"
                     /translation="MRTPWRSLFALVLAFAGTSGAGLPITLSMDEVVGVRGQTVTMPV
                     RITSEPLVAADSIVSGQFDLVFDPTVLVPDTMLSRQASGGTIMSKSMVAANVEPGMLR
                     LAFASADAQPYVGDSVVVRIVFHVIGQHGQESSLEIHNILFNELPGGATEPGMGWVLV
                     QSAPPVFTSFPSAVTVMVGQLMQVQVKAVDDDGAVFYSSQFTQGTQLFSINGETGVMA
                     YTPVPADVGSYTIRVTAWDVFEISTSREFRLTVLADTTKPVIIGSPSATGITENAATI
                     VWNTDKLSTSMVRYGLTGGAMTEAGNTTLTRQHAVPLTSLLPNRGYSYEVWSVDVFGN
                     SSAVKTGTFTTLQAPQTEPLVITLRPSVERGTNQAAVSWETNRQSTTVVRYGTSSAAL
                     TDSVVGVSGSTHTATIKGLTPNTKYFFRVRSVDAWNLSVLSAIDSFTTKALLLAPVIT
                     TPPSVIARDHTSFTVRWVTDRASTSVIDVGTTAAYGRRISDTTAVTVHIVAVTNLAPS
                     TQYNYRAGSAGPEGLGPTFSANQQVYTTDAPDTKPPVITQGPVDQGVDNESATIYWLT
                     DEAANAIVDYSVVQPDGSADFGQRVVRMVYEKSHSVVLTNLLPSTTYRYRVSSFDLIG
                     NGPTQAIAEFRTAAAPDLTPPVLLGRVQVSGITERSAMVRWATDEASDSRVLYTAQSA
                     VPEAVTVSALVTDHVVSLTGLAPSTNYTLSVYSTDIAGNPLGPIAASFRTKAVPDTIP
                     PAITALPKVDGQTLTSFNLVNVVMSLGTNEVATAQAVFGFNQAALVGMVPASAPGLTQ
                     VLQFTNLPPDTTVFYRITVTDAAETPNSRVDIVRSFRTIKGADLTAPAITVGPVVTDV
                     QERSFTVAWTTDEASTSRLRFGTVATNLVGNAQDPTPVQSHRITATNLTPGTKYFWRV
                     LSADPSGNTVASSVREVTTPVERDITPPVFVRRPVATYAAFDNAVITWATDENTTAYI
                     YYAGSTDTVFNVAVDENYRTDHIVSITNLRGGVEYVYYVEAMDNAGNSAVAGDTSAPV
                     FQNNGKITRLGDGTAKITQAPRGGTFTTTVTPDVTYPVITGDPNVVETNSTSTTVTWT
                     TDETSNSVVRILETSGGAGKAARAADITADLLERGRVIAQSDNVKNHQLTITSLTPGA
                     SYVLHVTSTDPSGNGETISQPVYVTLPQAADLTPPSIVEGSVTATMTQTQAIISWQTD
                     ELSDSRVDYAQGSVTELTLSRVVPDPVKNHQVVLTNLLPDQLYSYRVFSTDIRGNGPA
                     SSGIHTFTFRTAAQPDVTPPVISGVTVTGVTDVNANITWQTDEDADAFVEYGTTPSFG
                     LTRNEPVLKKSHTLTLTNLAPATKYYFRLAGSDRAGNTSALTPLDSLVTAATPDLTPP
                     DAPAVVDTIPASARVILRWAKSASPDVASYIVYRNGSILASGVGDTAYVDRSVANGIT
                     YTYEIAARDFAGNVGTKSPAVRETPQATEAPQAPEPKSPILGKLQQAETVVFEVNNAV
                     RAVSRPTVPLTYSFVVASDSLLQNVIAAGNNIAEGSGTTSWSVSGSQFEMNKVYFWAA
                     QASDGVAVGPITTSIRFRYGDFTSVELQSFTATGTPNGAVKVSWTLRVSNAANLRVAL
                     MKGPDVSSAREIRRYESGGLAGELMDITARLGESSRYWLQTTDGMGSVRTFGPVEATA
                     ALPTSWELSSARPNPFNPTTEMMLSVPAAGEARVAVFNILGQPVATLLSGRVDAGIHR
                     VVWNGRDTEGRSVSSGVYFVQLQTGSGVRIVRRVTLLR"
     gene            complement(6939..8333)
                     /locus_tag="PLT86_05875"
     CDS             complement(6939..8333)
                     /locus_tag="PLT86_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT36174.1"
                     /translation="METRFEPTQYLVQAAADALLEYENGPDLTFAAGDWEAVFGAAYR
                     WFRAFGKESLEDLSQNAPDSVTAGEKLLNECPDRLAMLVADALTTNSIAPPVCISLTP
                     VHLDTPYGAVVFAAFPRAIEELAPLLVDGLEEQMRSRRFSGVVAIHDTWMQKDGYPER
                     ILRLDAENNVIVEQGVLNPCRPERILLLLGPGSDEATWHRRWHDVASNCGIDVANPYE
                     FAYIADDKWQTFHIWQNAGVPTPVTRLVPPDTAFTAEMCLSLVDGPSAPVVAKPRHGT
                     EGLAATVCNPAETTAPLKAIWERGDDAVLQPFTNDLVWVEEAGEAHTAVLRMNVTCNA
                     QGASRVESGFVHVAARGCGIASAGHQGRLRPIVRSALRVRVGGGLAPFPAHLAAQCEA
                     CACAAVDAVAATSRKTRLAGVDVVVCAGDGGMPRALALEVNPRPAGLSHSRFLSAGWE
                     TEGEPGVSRFMWGE"
     gene            complement(8379..15821)
                     /locus_tag="PLT86_05880"
     CDS             complement(8379..15821)
                     /locus_tag="PLT86_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36175.1"
                     /translation="MATTRERSAKKKEAPSQTHERVFFPVRCAAELDGPQAGNRSRFA
                     GRAKPAASEIAALLCGKLWGRAAKDIVLVKDAFSVCAVRQSTPPRVEIAGDVLEALGL
                     RLSGKQITGPAETVSGAIAVLRLAKAWSDEFRRTSDARAALRAALDAWADFPATDREA
                     CLDVLAMPPLDTGKVFSAFLRLAGDEKTRSQQGESADAWRERWITWVLGQMRIDLPYD
                     RTAVRDILSSVDDPDESRAMLYQAIRKYDRGIEQDNAARLAEELAWAREELIFGRMSR
                     AFHNQALLFASSRYITVTAEMVRAGARLAAAASVWPALRPAVDRVTLLLQPHMEVPHT
                     ELAGAIDHVEDVAVQQQKTYVQKFLEQERGQSSQAGATEGRTGALEAAYSSGKRILAS
                     TRAIRASLAKSPRRPAAYVVISQRPSPTGSHLVARINEGQDPFLGKPEDLRELVRYAG
                     DRLYASPDYAWLEYANHWIEAIPLFIKERIRVDNGVEIAETVIDQEGMEESFREQMAG
                     YWERNIARTELSGMAALARALMSGEDPEGSDLSLSADLSRARKALRNGGAGQIARLTA
                     ALAIGAETNAFEISMAAESSGDRLEATRRVLFGGDAKPGLWTSVLEIRKHFADWPKAV
                     DEALSKASENDKNRFNSLIPEAERLATIPRRPLPVLHVLTTQSARMTDGYIRTWLEES
                     MALSRIAREYGLDADVRQRMDDYAREIEAIAESLVKELGLEPEIESIMATERVNRRAA
                     ILRLVGANRIFQKQVSLIAVLREVRGVAKGDLNAPLDSPELDAFTAEHEREISDKALA
                     KVVERNVDEFARSIAEMGASASREDLHSLQRKVVAENSDLRADLDTFTRFESREGFFE
                     DLSARHPDWKLKERRETWLRKHQRLALTTARKEIIAEQGLNHLTLDPRFFLEAVGGNK
                     RYHLIYTPSRVDLGPKERESVETWSQWVGGRDKEAAQAGRRIYGLINKSVKRFNSLTE
                     PEILKTGENASMAAHFAYSNAMALMVNAAGRGDMEQLGDEMSLRNDRIIHPAGEGYGG
                     YCVPKDGLFLEFVLTLSERVKLRQLGIEERFHETVSQLARKILLKREEFPCDLEWERW
                     AEELLGREEGLQGLFSTHSGENPDAANLRIPVFQITRLATVLEQLGQPPLRDNRAVVK
                     SLAARWGIHTMVAGAEHVNRFMPFYKAWLIYDTIAEARRFDPSVASPEESVIVLSAEY
                     KPDTQDGRFSVGMRKYEILAGTGDHLRYSLGYEAATLASLMFDGWSVVESRRGVDDPV
                     LEQIASAWVGNKDDVRSALKSVFPGYGPPAEMRLVSPMGLSKHDVLRYTSDTRLEEYA
                     EEARVALVTAGLSDEDIRAHMATFGPRLERWTRLRNRGAAELAGCLKGKVHALALAVL
                     GPERSYEHALQGADVFDVGIPHRQVMELLNEPARLCDLMLFGKPGSALAIVDGASGAR
                     RRALNRRGVMLWFAAAENRGKRGVYRSIGLGEDTVEEWRSQMREQRQRALEYADALRA
                     GRKAVDSAFERVITQLRSEQEASLVLEEEERLRRFSRDTERDHVVAMLLAEIVTMRSP
                     RELTFAHWLALGGMFVLEGCSPAAFEEGYVSFEKARGKLQPGSEEPRTAAEIKRLAGL
                     ISRPLVRAYGSGFRQVGGVESSNKATEEVKHIALSTRRQLADRARRLMSARVRADSFQ
                     TTWNEPRLRHKSLEELNADVRETLGEPKVFASEALLGKLVALARVFGEKLLAEICLLA
                     SPEREKAVVAIATLLNEREFDVSALKGVCGSYEDIGTIGRMAQELSERAARENWTAEA
                     SDAAFDKLADFAEFVSTIHALEISLADRQRNPDEVDAPSLWRQLASYFAETLNDHFYE
                     YRPWAYSRGVGFQRYTGDRLYALANRHFAWLYRYLRHLIVTRTEVRYLTPVQQDLLIG
                     RISETGVVVAIGASGDTEEEKRWRAFNQLREIAFIRNDGFPLPPTFDGFDPALIDADN
                     RANILSMHPVGRTHVSRLVAEGPTLARELVVAGQPAANVILTRSVRVDRDSVLVDDGH
                     LYVDKQTYVQALRDNWGLSETGAKALAERDVGPKGVRIAVRFSRPVRAAVVLPMHGNP
                     VYDGGHLERLGLPYSVQSRFHTWTTYDKAKYPDIFTPETGVRIPAEIDWLHEWTVAGE
                     EEEIKHQIRSGRPGTDYCGLQRFSEKYGIVMVKDAAESGGRGQKPFPLRTAVGSLNEE
                     TLSEAVDFLYQISLVHNVSIQEVVLSSPETWATEEFLERFVDRQVTEWYRPITRDRQP
                     ATPLFGSLRVIVSTDRPLAEDRYHHWHMSHRISLNSTQLITNVGRGGTLDLLRPEDIR
                     PEFRDTILAALDDAARRTMEAMAAYEAKAGARYTLETGLPVGKDASGVSYGIPRYLML
                     DFLLRPVFHRKGDVVETVPRIDEKGNRIGSVFMLRDGNDVFEGEIVDWEVILIEPNIG
                     IGLWDRVAIREEELERKRAQATGRPMDWDRVGENARVVLRDLTRAGIDYLEAKRRGST
                     "
BASE COUNT         3118 a         4998 c         4712 g         3374 t
ORIGIN      
        1 ctggcaacgg atcaaagcag acgctggccc tggttccggc gcggttcaca gtgaatgaca
       61 aagccgttgg gggcgcacat gaaggggtcc cgatgtcggt ccggtaacac cttgacgctg
      121 gtgctgttcc tggccgcttc ttggattctg ccgtccaccg cacgcgcgaa atcccttggc
      181 gaaatcgttc tgagcgtgcc ggtcgacacg gtcgtggaag cgggacagca gtttttgttg
      241 ccggtacgca tcagcggcgt caccgttctg gactccgtaa cctccttcaa cttcggcgtt
      301 cgcgtcccgc tcggtatcat gttggtagat actgcagccg tcaaaggacc agctctcccg
      361 tctggagcgg ggtttagctc cgcgaaacgt gacagtgtga ttgtcgtcgg gggctactgg
      421 acactcccgc tggcaaacaa cggcacgttg ttctttctgc gttgcactcc gaaggccaac
      481 attccggaca acacctccgg ggaggtgcga ctggaacggt tatcgcagaa cggcactctg
      541 ctctactatt tgaacgaacg aaacaagcct ccctgtaccg cgcagaatgg gtctgtacga
      601 gtccgcaacc tgaacatggt gccagtccgc atcgctgttt cgtcagattc ctcggttgac
      661 gcagggtccc cgtgttccat tcaggttcag ggcagcggcc ttatttcctt cagcggcacc
      721 acgacgctgc aatttgctct tacggttccg cccgggctct accagtcggt atccgttcag
      781 ggagggcttc ttcccgcgga cgcagtcaca ctcgccgacg cagggcgcgg tctgttccgc
      841 gtttcgggat ccgtcgccaa tcccgcggga ggtacgggca ccctgttctc cgtgcagggg
      901 ctcacaaagc aggacatccc tgacagcacc gtattcgcga ttgcaatcga acggctgtcg
      961 gacgggggca gcccccttcc gtacgtccgg aaaggtgatc agacaccgga ctgcacggta
     1021 tcgaacggcg cggttaccgt ccgaaggccc gtttcggttg cgcatggagg agcctcggtt
     1081 cccgcgccaa gcgagctgac cgcgtatccg aatcccttca atgcagtcat cacattccgg
     1141 gtgcacgggc cgctgtcgga cgggcccatt gacgcgaccc tttacacggt gcttggaacg
     1201 gtggttcgcc gctggtcaca atcgaccccg gaatggacgt gggatgggcg ggatgcctcg
     1261 ggcgttccct cggcaagcgg cgtctacgtg gtggtcgtaa gaaacggaga aaaaacctgg
     1321 cagaagagag taacactctt gcgctgaagt gcatatattg taaagggaat gcgttcggtt
     1381 catgatgtcg cgggggcatt gatacgcacg gtcatctggc caatgattcc atcggaatgc
     1441 gcacagagcg tgaccggaac ttacccggcg aaatggcagt tctgtttcgt tagcgattat
     1501 cggttttccg tttttcctat gccggctggc acctgacagc agggagggta gctcatgaga
     1561 accccttgga gatctctttt cgcgctggtc cttgcgttcg cgggaacgag tggcgcgggt
     1621 ctgcctatca cgctttccat ggatgaagtg gtgggcgtgc ggggtcagac cgttactatg
     1681 ccggtgagga tcacgagcga acctctggtg gcggcggaca gcatcgtaag cgggcagttc
     1741 gatcttgtgt tcgacccgac ggtgctggta ccggacacca tgctgagcag acaggcttct
     1801 ggcggcacca tcatgtcgaa atcgatggtg gcggcgaatg ttgagccggg gatgcttcgt
     1861 ctggcgtttg ccagcgccga tgcacagcct tatgtcggcg attcagtcgt ggtgcgaatc
     1921 gtgttccatg tgattggcca gcacggacag gagagctcgc tggagatcca caatatcctg
     1981 ttcaacgagc ttcctggcgg ggcgacggaa ccagggatgg ggtgggtgct tgttcagagc
     2041 gcccctcccg tgttcacatc gttcccctcc gcggtaaccg tcatggtcgg ccagttgatg
     2101 caggtgcagg tcaaggccgt ggacgatgac ggcgcggtgt tctacagcag ccagtttacc
     2161 cagggaacac agcttttctc catcaacggc gaaacgggtg tcatggcgta cacgcccgta
     2221 ccggcggatg tcgggtccta cacgatcaga gtgaccgcgt gggacgtgtt cgagatcagc
     2281 acgtcgcggg aatttcggct gacagtgctc gctgatacga cgaaaccggt gatcatcggt
     2341 tcaccttccg cgacaggcat cacggaaaac gccgccacga tagtctggaa caccgacaag
     2401 ctttccacct cgatggtgcg gtacgggttg accggtggcg cgatgacgga ggccggcaat
     2461 accacgttga cgcggcagca tgcggtccca ttgacctctc tcctgccgaa ccgcgggtac
     2521 tcgtacgagg tctggtcggt tgacgtgttt ggaaacagct cagcggtgaa aacaggtacc
     2581 ttcaccaccc ttcaggctcc gcagaccgaa ccgctggtga tcacgctgcg cccgtcggtg
     2641 gagcggggaa caaaccaggc ggcggtttcg tgggaaacga accgtcaatc cacgacggta
     2701 gtgcggtacg gaacgtcttc cgccgcgctg acggacagcg tggtcggggt ttcaggaagt
     2761 acccacaccg caacaatcaa agggctgaca cccaacacga agtacttttt ccgcgtccgg
     2821 tctgtagatg cgtggaatct ttccgtgttg agcgcgattg attcgtttac cacgaaggcg
     2881 cttctccttg cgccagtcat cacgactccg ccgagcgtga ttgcgcgtga tcacaccagc
     2941 ttcacggttc gctgggtgac cgaccgcgcc tccacatccg tgattgacgt cggcacgacg
     3001 gcggcgtacg gtcgccgaat cagcgatacg acggcggtga cggtgcacat tgtcgcggtg
     3061 accaaccttg ctccgtccac tcaatacaac taccgcgccg ggtcggccgg tccggaaggc
     3121 ttggggccga cgttcagcgc caatcagcag gtctacacaa ccgatgcgcc cgatacgaag
     3181 cccccggtaa tcacgcaggg cccggtggat cagggagtgg acaacgaatc ggccacgatc
     3241 tactggctta ctgacgaagc ggcgaatgcg atcgtggatt acagcgtggt acaacccgat
     3301 ggaagtgccg atttcggcca gcgtgtggtt cggatggtgt acgagaaaag ccattccgta
     3361 gttctgacga acctcctgcc cagcacaacg tatcggtacc gggtgagttc cttcgacttg
     3421 attggcaacg gtccgactca ggcgatcgcg gagttccgca ctgccgcggc acccgatctt
     3481 acgccgccgg ttctcctcgg ccgggttcag gtaagcggga ttaccgaacg cagcgcgatg
     3541 gtacggtggg ccaccgacga ggcatcggat tcccgtgttc tgtacaccgc gcaatcagcg
     3601 gttcccgagg ccgtaacggt ttcagcgctc gtgacagacc atgtcgtctc gctaactgga
     3661 cttgcgccgt ccaccaatta cactctctcg gtgtacagca ccgatatagc gggtaacccg
     3721 ctgggaccga tcgctgcatc gttccgcacg aaggcggtcc cggacacgat tccgccggca
     3781 atcactgcac tgcctaaggt tgacgggcag acgctcacat cgttcaatct cgtgaacgtg
     3841 gtcatgtcgc tcggaaccaa cgaagttgct accgcccagg cggtgttcgg cttcaatcag
     3901 gcggcgcttg tcgggatggt tccggcgagc gctccgggac tgacgcaggt gcttcagttc
     3961 accaatctgc cgccggatac caccgtgttc taccggatta ccgtgacgga cgccgcggaa
     4021 acgccgaact cgcgggttga catcgtgcgc tcgttccgta cgatcaaagg tgccgatctc
     4081 actgcaccgg cgatcacggt cggaccggtg gtgacggacg ttcaggaacg cagcttcaca
     4141 gttgcctgga cgaccgacga ggcatccacc agcaggcttc ggttcggcac cgtcgccacg
     4201 aatcttgtgg ggaacgcgca ggacccgact cccgtgcaat cgcaccgcat caccgcgacg
     4261 aacctgactc cggggacgaa gtatttctgg cgtgttctgt ccgcggatcc ttccgggaac
     4321 accgttgcct catccgtgcg cgaggtgact acgccggtag agcgcgacat cactcctccg
     4381 gtgttcgtcc ggcggccggt tgcaacgtat gctgcgtttg acaacgcggt gattacgtgg
     4441 gcaacggacg agaataccac ggcctacatc tattacgccg gctcgacgga tacggtcttc
     4501 aacgtggcgg tggacgagaa ctaccgaacc gaccacatcg tcagcatcac gaacctgcgg
     4561 ggtggcgtgg aatacgtgta ttacgtggag gcgatggaca acgccggtaa ttccgcagtt
     4621 gcgggtgaca cttcagctcc ggttttccag aacaacggga agatcacaag gcttggcgac
     4681 ggtaccgcga agatcacgca ggcgccgcgt ggaggaacgt tcaccactac cgttacgccg
     4741 gacgtaacct accccgtcat cacgggtgat ccgaacgttg tcgaaacgaa cagcacctcc
     4801 actacagtaa cctggacaac ggacgagacc tccaacagcg tcgtgcgaat tctggagacg
     4861 tcgggtggcg cgggaaaggc cgcgcgggcg gcagatatca ccgccgatct tctggaacgc
     4921 gggcgtgtga tcgcgcaatc tgacaacgtg aagaaccacc agttgactat cacctcgctc
     4981 accccggggg cttcgtacgt cctgcacgtg acgtccaccg acccgtcggg taacggcgaa
     5041 accatcagcc agccggtgta tgtcacgttg ccgcaggccg cggatcttac tcctccgtcg
     5101 atcgtggaag gctccgttac tgccacaatg acgcagactc aggcgatcat aagctggcag
     5161 accgacgagc tttcggacag ccgtgtggat tacgcgcaag gcagcgttac tgagctgacg
     5221 ctctcacgcg tcgtaccgga tccggtcaag aaccatcagg tggtgctcac gaatctgctt
     5281 ccagaccagc tctactccta ccgcgtgttc tccaccgata tccgcggaaa cgggccggct
     5341 tcgagcggca tccatacgtt taccttccgg acagccgctc aaccggacgt gacgccgccg
     5401 gtgatttccg gggtgaccgt gacgggagta acggacgtga acgccaacat cacgtggcag
     5461 acagatgaag acgcggacgc gttcgttgag tacgggacga cgccttcatt cggccttacg
     5521 cggaacgagc cagtgctcaa gaaatcgcac actcttacgc tcaccaacct tgcgccggcg
     5581 acgaaatact acttccgtct ggcgggaagc gaccgcgcag gcaataccag cgctcttacg
     5641 ccgctggata gcctggtgac tgcggctacg ccggatctta cacccccgga cgcaccggcc
     5701 gtggtcgaca cgattccggc ttcggcgcgt gtgatcctgc gctgggcgaa gagcgcgtcg
     5761 ccggacgtgg cgagctacat cgtgtaccga aacggttcca tccttgccag cggtgtgggc
     5821 gacacggcat acgtcgacag aagcgtggcg aacgggatca cctacacgta cgagatcgcc
     5881 gcgcgcgact tcgcgggcaa tgttggtacg aagtcccccg cggtgcgcga gacgcctcag
     5941 gcaaccgaag caccgcaggc accggaaccg aagtcgccga ttctggggaa actgcaacag
     6001 gcggaaaccg tggtcttcga ggtaaacaac gcagtgcgtg cggtttccag acctacggtt
     6061 ccgttgacgt attccttcgt cgtggcaagt gattcgctcc tgcagaacgt cattgcagcg
     6121 gggaacaaca tcgcggaggg ctccggaacg acgtcgtggt ccgtttcggg cagtcagttc
     6181 gagatgaaca aggtctactt ctgggctgct caggcttcgg atggagtggc cgtgggaccg
     6241 attaccacct cgatccgctt ccgatacggc gatttcacga gcgtggagct tcaaagcttc
     6301 acggccacgg gaacaccgaa cggcgcggtc aaggtgagct ggacgttgcg ggtgagcaat
     6361 gccgcgaatc tgcgcgttgc tctcatgaaa ggccctgacg tttcctcggc ccgtgagatc
     6421 cgccgttacg aatcgggtgg acttgccggg gagctgatgg acatcaccgc ccgtctcggc
     6481 gagtcttcac ggtactggct gcagacaacg gacggcatgg ggtcggtgcg caccttcggc
     6541 cctgttgagg cgactgcggc gttgcctacg tcatgggagc tttcttccgc tcgtcccaac
     6601 ccgttcaacc cgaccacgga aatgatgctt tctgttccgg ctgccggcga ggcgcgtgtg
     6661 gcagtgttca acatcctcgg ccaaccggtg gcgacacttc tctcgggccg cgtggacgca
     6721 ggaatccacc gcgttgtctg gaacggccgt gataccgaag gacgttcggt ttccagtggc
     6781 gtgtatttcg ttcaattgca gacgggaagt ggcgtacgga tcgtgcgtag ggttacgctg
     6841 cttcgttagg ctccttgccg ccagaaaaaa gaggcgctcc ggaattgctc cggagcgcct
     6901 tttttgctcg cgtctcgctt catagtcatc gttccggctc attcacccca catgaatcgg
     6961 gaaacgcctg gttctccttc ggtttcccat cccgctgaaa gaaatcggct gtggctaagc
     7021 ccggccgggc gagggttgac ttccagcgcg agggcacgcg gcatgccgcc gtcgccagca
     7081 caaacaacta catcaacacc tgcaagcctc gtcttgcgcg acgtagccgc cacggcgtcg
     7141 actgccgcgc acgcacacgc ctcacactgc gccgcaaggt gtgctggaaa tggggccaag
     7201 ccgccgccga cgcgcactct cagtgcggac cgaacgatcg gcctcaatct gccctgatgg
     7261 cctgcggacg caataccaca accgcgtgcc gcaacgtgca cgaatcccga ctcgacgcga
     7321 ctggcgccct gtgcgttgca cgtcacattc attcggagca cggcggtgtg agcttctccc
     7381 gcctcttcca cccatacgag atcgttcgtg aacggttgaa gcaccgcatc gtcgccgcgt
     7441 tcccagatag ctttcaatgg ggcggtcgtt tcggccggat tgcacaccgt ggcagcaagg
     7501 ccttccgtgc catgcctggg tttggcgaca acaggcgccg acggtccgtc caccaggctg
     7561 aggcacatct ccgcagtgaa cgccgtatca ggtggcacga ggcgcgtaac cggcgtcggc
     7621 acaccggcgt tctgccagat gtggaatgtc tgccatttgt cgtcggcaat gtatgcaaac
     7681 tcgtagggat tcgccacatc aatgccgcaa ttgctggcga catcgtgcca ccgtctgtgc
     7741 cacgttgctt cgtccgaccc gggcccgagc agaagcagta ttctctccgg cctgcatggg
     7801 ttcagaacgc cctgttcgac gatgacattg ttctctgcgt cgagacggag tatgcgctcc
     7861 gggtacccat ccttctgcat ccacgtgtcg tggatggcga ccacgccgga aaacctgcgc
     7921 gaccgcatct gctcttccag accgtcaacc aggaggggcg cgagttcttc gatcgcgcgc
     7981 gggaacgctg caaacacgac cgccccatag ggagtgtcaa gatgtacagg cgtcagtgag
     8041 atgcacacag gcggggcaat ggagtttgtg gtaagggcat ccgccacgag catggcaagg
     8101 cggtccgggc attcgttcaa gagcttctcg ccagccgtaa cggaatccgg tgcgttctgg
     8161 ctcaggtctt ccagcgattc ttttccaaat gcgcgaaacc agcggtatgc tgctccgaaa
     8221 actgcctccc agtcgcctgc cgcgaatgtt aggtccggtc cgttttcgta ttcgagaagc
     8281 gcatctgcgg cggcttgaac gagatattgc gtgggttcga atctggtttc caccgggtgg
     8341 caacccctct cggcgtggtc ggcactacct tcagagcttc aggtactacc gcgccgtttc
     8401 gcttcaagat agtcgatacc ggcgcgggtc aggtcccgaa gaaccacgcg cgcgttctcg
     8461 cccactcgat cccagtccat gggccgtccg gttgcctgtg cacgttttcg ttcaagctct
     8521 tcttctcgaa tcgccacacg gtcccaaaga ccgatgccga tattcggctc aatcaggata
     8581 acctcccaat cgacgatctc gccctcgaaa acgtcattcc cgtcccgcag catgaagaca
     8641 gatccgatgc gattgccttt ctcatcaatt ctgggaacgg tttctactac atcccctttc
     8701 cggtggaaga cgggacgaag gagaaagtcc agcatcaagt accgcggaat tccgtatgag
     8761 acaccggacg cgtctttgcc aacgggtaat cccgtctcca acgtatatct ggcgcctgct
     8821 tttgcttcat atgccgccat cgcttccatc gttcgccggg ccgcatcgtc caacgcagcg
     8881 agaattgtgt cccggaactc cggccgaata tcctccgggc ggaggaggtc cagcgtgccg
     8941 ccacggccca cgttggttat cagttgcgtg ctgttcaggg agatgcggtg gctcatgtgc
     9001 cagtggtggt acctgtcttc ggcaagcggc ctgtccgtcg agacgatcac ccgaagcgat
     9061 ccaaaaagag gcgttgcggg ctgccggtct ctggttatcg gtcggtacca ctcggtcacc
     9121 tgtctgtcca caaatcgctc gaggaactcc tcggtggccc aggtttcggg agacgaaaga
     9181 accacctcct ggatggaaac gttgtgaacg agagagatct gatagaggaa gtctactgct
     9241 tcgctcagtg tttcttcgtt caatgatccg acagccgtgc gcaacggaaa cggtttctgc
     9301 cctcttccgc ccgattctgc agcgtctttg accatcacga tgccgtactt ctctgaaaac
     9361 cgctgaagtc cgcagtaatc ggttcccggt ctcccggaac ggatctgatg cttgatctcc
     9421 tcctcttccc cagcgaccgt ccattcatga agccagtcaa tttcggccgg aattcttacg
     9481 cccgtttccg gggtgaaaat gtcggggtat ttggccttgt cgtacgttgt ccacgtatgg
     9541 aaccgcgact gaacgctgta cggaagcccc agtcgttcga ggtgtccccc gtcgtagacg
     9601 ggattcccat gcatcggcaa aacaactgcc gccctcacgg gtcgcgaaaa ccgaaccgcg
     9661 atccggacac cttttggtcc gacatctcgt tcggcgagcg cctttgcacc ggtttcgctg
     9721 aggccccagt tatctcgcag cgcctgtacg taggtctgct tgtccacgta aaggtggcca
     9781 tcgtcaacca gaactgaatc acggtcaaca cgcactgacc gtgtcagaat cacatttgcc
     9841 gccggctgac cggcaacaac cagttcacgc gcaagagtgg gcccttccgc gacgagacgt
     9901 gatacgtggg tgcgtccgac cggatgcatg gacaggatat tcgctctgtt gtctgcatca
     9961 atcaaagcgg gatcgaagcc gtcgaacgtc ggagggaggg ggaacccgtc gttccgaatg
    10021 aacgctattt cacgaagttg attgaatgct ctccaccgtt tttcctcttc ggtgtcaccc
    10081 gatgcaccga ttgccacaac aaccccggtt tcgctgatcc tcccgatcag aagatcctgt
    10141 tggacaggcg tcagatacct cacttccgtc cgagtgacaa tcaggtggcg gaggtaccga
    10201 tacaaccatg cgaaatggcg gttggcgagg gcatacagcc ggtctcccgt gtacctctgg
    10261 aagcccaccc cgcgtgagta tgcccacgga cggtactcgt agaaatggtc gttcagcgtc
    10321 tcggcgaaat aagatgcgag ttgccgccaa aggcttggcg catccacctc gtcaggattg
    10381 cgctgtcggt cggccagtga tatttcgagg gcgtgaatcg tgctcacaaa ctccgcgaaa
    10441 tcagccagtt tgtcgaacgc cgcatcactt gcctccgcgg tccagttctc ccttgcggca
    10501 cgttcggaca actcctgtgc cattctgccg attgtaccaa tgtcctcata acttccgcag
    10561 acacctttca acgcggaaac gtcgaactcg cgctcgttca agagcgtggc aatggcgacg
    10621 actgcctttt cccgttccgg cgatgccagc aggcaaatct cagcaagaag cttctccccg
    10681 aagacgcgcg caagagcgac aagttttccc agaagggctt ctgaggcgaa gactttaggt
    10741 tctccaagag tctcgcgcac gtcggcgttg agttcttcca ggcttttgtg tcgaagccga
    10801 ggttcattcc acgtggtctg gaaggaatcc gcgcgcactc gtgcgctcat caatctgcgt
    10861 gcgcggtcgg caagctggcg ccttgtgctc aacgcaatgt gtttcacctc ttccgtggct
    10921 ttgttcgagc tctccactcc gcccacctga cggaatccgc tcccgtacgc acgcaccaga
    10981 ggacgactta tgaggccggc aagtcgcttg atctccgcgg cggttctggg ttcttcggaa
    11041 ccgggttgaa gtttgccacg cgctttttcg aacgaaacat atccctcctc aaaggcggca
    11101 ggagaacacc cctcgaggac aaacatgcca cccagtgcaa gccagtgcgc gaatgtcagt
    11161 tcccttgggc tccgcatcgt tacgatttcg gcgagcagca tcgccacgac atgatcgcgc
    11221 tcggtatctc gtgaaaacct gcgaagccgc tcctcctctt ccaacacgag cgaagcctcc
    11281 tgctcactgc gaagctgcgt gatgacacgt tcgaatgccg agtccaccgc cttccggcct
    11341 gcacgcaacg cgtccgcgta ttccaacgcc cgctgacgct gttcacgcat ctgggaccgc
    11401 cattcctcga cggtgtcttc accaagccct atcgagcgat acacgcctct tttcccgcgg
    11461 ttttccgcag cggcaaacca gagcatcaca ccccggcgat taagcgccct ccttcgcgcg
    11521 ccagacgccc catctacgat cgcaagggcg cttcccggtt tcccgaacag catcaggtcg
    11581 cacagacgcg ccggttcgtt cagcaactcc ataacctgcc tgtgcggaat tccgacatcg
    11641 aacacgtctg caccctgcag cgcgtgttca tatgaacgct ccggccccaa tactgccaat
    11701 gcgagcgcgt gcactttccc tttcaggcac ccggcaagtt cggccgcgcc gcgattgcgc
    11761 agcctggtcc atcgttccag tcgggggccg aaggtagcca tatgagctcg gatgtcttca
    11821 tcggacagcc ccgcagttac cagcgcgacc ctcgcttcct ccgcatactc ctccagtcga
    11881 gtgtcgctcg tgtaccgcag cacatcatgc ttcgagagcc ccatgggact aaccaggcgc
    11941 atttccgccg gaggtccgta gccagggaac acggatttca gggccgaacg cacatcatcc
    12001 ttgttgccaa cccatgcact ggcaatctgt tcaagaacgg gatcatccac gccacgtcgg
    12061 gattccacga cggaccaccc atcgaacatc agcgacgcga gggtggcggc ctcgtatccg
    12121 aggctgtagc ggaggtggtc acctgttccg gcgaggattt cgtacttccg catgcccaca
    12181 gagaatcgcc catcctgcgt gtcgggcttg tactccgcgg aaagcacaat cactgattcc
    12241 tcaggagatg ccacagacgg gtcgaaacgg cgggcctcgg caatggtgtc gtatatgagc
    12301 cacgctttgt aaaaaggcat gaaccgattc acgtgttccg cacccgcgac catggtgtgg
    12361 ataccccatc gggccgcgag gctcttcacc accgcgcggt tgtcacgcaa tggtggctgc
    12421 cccagttgct cgagcacagt ggcgagacga gtgatctgga acacggggat acggagattc
    12481 gccgcgtccg gattctcccc cgagtgtgtc gaaaaaagcc cctggagccc ttcctctcgc
    12541 ccgagaagct cctccgccca gcgttcccat tccaggtcac acgggaattc ttcccgtttc
    12601 aggaggattt tgcgtgcaag ttgtgaaacg gtttcgtgaa accgttcttc gatgccaagc
    12661 tggcggagtt tgacgcgttc agaaagcgtg agaacaaact ccagaaacag cccatccttc
    12721 ggcacgcaat agccaccgta cccctccccg gcaggatgta tgatgcggtc gttgcggagg
    12781 ctcatctcgt cgcccagttg ttccatatcg ccacgcccgg ccgcattgac catgagcgcc
    12841 atggcgttgg agtaggcgaa atgcgccgcc atggaggcat tctcaccggt tttgaggatt
    12901 tctggttccg taaggctgtt gaaccgcttc accgacttgt tgatcaggcc gtatatcctt
    12961 ctcccggcct gagcagcctc cttatcgcgc ccgcctaccc attgcgacca ggtttcgact
    13021 gactcccgtt ccttgggacc aaggtccact cggcttgggg tgtaaatgag gtggtaacgc
    13081 ttgtttccac ccactgcttc caggaagaaa cgtggatcga gcgtgagatg gttcaggccc
    13141 tgttccgcga tgatctcctt ccgagcagtt gtcagcgcaa gcctttgatg ctttctcagc
    13201 cacgtttcgc gtcgttcttt gagcttccag tcaggatggc gtgccgacag gtcctcgaaa
    13261 aacccctctc tgctttcgaa acgcgtgaac gtatcgagat ccgcgcggag atcgctgttt
    13321 tcggcgacta ctttgcgttg gagggaatgc aggtcctccc tagaagcgct ggcacccatt
    13381 tcggcgatgg aacgtgcgaa ctcatcgaca tttctttcga ctaccttggc gagggctttg
    13441 tcggagatct cccgttcgtg ttctgcggtg aaagcgtcaa gttcaggtga atcgagagga
    13501 gcgttcaaat cccccttggc tacgccgcga acctctctaa gtaccgcgat caaactgacc
    13561 tgtttctgga aaatgcggtt ggcgcctaca agccgaagaa tggcggcgcg cctgttcaca
    13621 cgctctgtcg ccatgatgga ttctatttcg ggttccagcc ccagttcttt caccagagac
    13681 tcggcgatgg cttcaatctc gcgcgcatag tcatccatgc gctgcctgac gtcggcatca
    13741 aggccgtact cacgagcgat gcgggacagg gccatacttt cttcgagcca ggttcgtatg
    13801 tacccgtccg tcatgcgtgc ggattgtgtg gtaaggacgt ggaggaccgg caaaggtcgt
    13861 cgaggaatgg ttgcgagacg ttctgcttcg ggaataagcg agttgaaacg attcttgtcg
    13921 ttctcgctgg ccttcgacaa cgcctcgtcc actgccttgg gccaatccgc gaaatgcttt
    13981 cgaatctcca gaactgaggt ccacaggccg ggtttggcgt cccctccgaa taacaccctt
    14041 cgggtggctt ccagcctgtc gccggacgac tctgcagcca tgctgatttc gaaggcgttt
    14101 gtttctgcac cgattgccag cgcggctgtc aatcgtgcta tttgtcctgc tccgccgttg
    14161 cgaagcgctt tccgcgcacg ggagagatca gcggagagcg aaaggtcact tccttccgga
    14221 tcctcaccgg acatcagtgc cctcgcgagt gctgccatgc cactcaattc ggttcgcgca
    14281 atgttccgtt cccagtagcc cgccatctgt tcacggaaac tctcttccat cccctcctga
    14341 tcaatgactg tttccgcgat ctcgaccccg ttatcgactc ggattcgctc cttgatgaac
    14401 agcggaattg cttctatcca gtgattcgcg tactcaagcc acgcgtagtc gggagacgca
    14461 tacaacctgt caccagcgta acggaccagt tcgcgcaggt cctcaggttt tccaagaaac
    14521 ggatcctggc cctcgttaat acgtgccacg aggtgggacc ctgttggtga cgggcgctgg
    14581 ctgatgacta cgtacgcggc gggtcttcga ggagatttcg ccagcgaagc tcgaattgcc
    14641 cgtgttgacg cgagaatgcg cttgcctgag gaatatgcgg cttccagagc gcccgttcgc
    14701 ccctctgtcg caccggcttg ggagctctga ccacgttcct gttcgaggaa cttctggacg
    14761 tacgttttct gctgctggac ggcaacgtcc tccacgtgat cgatcgcacc ggcgagttcg
    14821 gtgtgaggca cctccatgtg aggttgcagg agcaaagtca ctcgatctac cgccgggcgg
    14881 agggccggcc atacagaggc cgcggcggcg agtctcgcgc ccgctctcac catctcggca
    14941 gtcactgtga tgtaacggga actcgcaaaa agcagcgcct gattatggaa cgcacggctc
    15001 attcgcccga agatcagttc ttcccgcgcc cacgccagct cctccgccaa ccgcgccgcg
    15061 ttgtcctgtt cgattcctcg gtcgtacttc ctgatagcct ggtagagcat cgcccgcgat
    15121 tcatcggggt catccaccga tgacagaatg tcgcgaaccg cagtcctgtc gtacggcaga
    15181 tcgatccgca tctgtccgag gacccatgta atccagcgtt cgcgccaggc atccgccgac
    15241 tcgccctgct gggatcgcgt cttttcatcg cccgccagtc gcaggaacgc ggagaacact
    15301 ttcccggtgt ccagaggagg catagccagc acgtccagac acgcttccct gtcggtcgcc
    15361 gggaaatccg cccaggcatc gagtgcagcg cgcagggccg cacgcgcgtc tgaagtgcgt
    15421 cggaactcgt cggaccaggc tttggcgagg cgaaggacgg caatcgcccc agagacagtc
    15481 tctgcgggcc cggtgatctg cttcccgctc agtcgcaggc cgagtgcttc caggacatct
    15541 cccgcgatct caaccctggg tggagtgctc tgcctgactg cgcacacgct gaacgcatcc
    15601 ttgaccaaga cgatgtcttt cgccgcacgg ccccacagct tgccgcagag caacgccgcg
    15661 atttcggaag ctgcaggctt cgcccttccg gcaaatcggc tcctgttgcc cgcctgcggg
    15721 ccgtccagtt cggcggcaca acgaacggga aaaaaaactc gttcgtgagt ctgcgacggt
    15781 gcttctttct ttttcgcgga acgctctcgt gttgttgcca tgagtacccc tggatgaagt
    15841 caggccggtc gcacccaatt cgagagatac gactggaatt ttgctttcgg aaaagatacc
    15901 cctgcgggct ctgacggggc aacgataacc gctcatgggt gcgtactcgt cgcggagcga
    15961 gcggcctctg ctgcaacgcc cgtgcgccgc ttgtttcccg aatgtgcggg gtgtagtttc
    16021 catgtatgtc cactcggcca ttctgtcgca ttgtacgccc tgccagcagt ttcctgcgga
    16081 acggcgcacg agcaaccttc gcggtcatcc tgtcggtgtt cgcgtgcagt cgggccgggg
    16141 cagtaactgt gtccgccggg gccggcttcg gggttcccac cgcgatggga gggtacctca
    16201 ag
//
LOCUS       DAOSOO010000057        15637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3773_length_15637_cov_5.269478, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000057 DAOSOO010000000
VERSION     DAOSOO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15637
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3773_length_15637_cov_5.269478"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLT86_05885"
     CDS             <1..769
                     /locus_tag="PLT86_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36176.1"
                     /translation="HDTEAGENLLWTALRTDAADCALSDASTLTTWPPPPWVRAVAFL
                     ARMNPRFALLHRSSVKLSDLPAAPRLSTSSGLARVQFDNAKPGAEWVELSGDLPTRLH
                     KLHLGHADGVIEPASDLLTLGIAEDAIEFLSTDMLLPAPGQGVWIVFSLSDRVDVERV
                     FRHLDDRKAGICAKAERTFTAQLDAPANCLVGARAWVQTKRLTLDACIMPAEGGVVQR
                     LSISGAPEQPEELVAGLVDMFRHQSVLRQCRGKADRG"
     gene            772..1230
                     /gene="aroQ"
                     /locus_tag="PLT86_05890"
     CDS             772..1230
                     /gene="aroQ"
                     /locus_tag="PLT86_05890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT36177.1"
                     /translation="MRILVLNGPNLNMLGIREPAVYGSQSLAGVEAELTSLASKLGVE
                     VECHQSNHEGALIDMIHAAFDRFQGILINPGALTHYSYALRDAIASVGLPAVEVHLSN
                     IHAREPFRHQSVIAAVCVGQISGFGVGSYQLGLRALASYLQKTQERTRKA"
     gene            1293..1366
                     /locus_tag="PLT86_05895"
     tRNA            1293..1366
                     /locus_tag="PLT86_05895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1327..1329,aa:Arg,seq:tct)
     gene            complement(1425..2252)
                     /locus_tag="PLT86_05900"
     CDS             complement(1425..2252)
                     /locus_tag="PLT86_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT36178.1"
                     /translation="MIKSINLWSFRDSNDLKTNVPLAKKAGFEAIELCLNEDTGPITW
                     KSTEKEVKEVRKFVEGAGLKIASLAIGTGWKYPLSSPDKKIVKAGADSIKKGVDVAKW
                     LGTDGVLVVPGVTTPEVPYDVVYKKTQDELKKLVPYAEKAKVAICVENVWNKFHLSPI
                     ELRDYVDSFNSEFLAVYLDVGNMLLYGYPEQWIRILGKRIKKVHFKDFRTGVGGFGGF
                     VGLLEGDVDWPAVMKAFKEVGYDGPASVEVGCYKHHWEASIYVIGLQMDFIFGRRSG"
     gene            complement(2236..2673)
                     /gene="acpS"
                     /locus_tag="PLT86_05905"
     CDS             complement(2236..2673)
                     /gene="acpS"
                     /locus_tag="PLT86_05905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT36179.1"
                     /translation="MSRIFGIGVDVVDVARIDRAANRWGDRFLKRVYTQRELEYCRER
                     PLPAEHLAARFAAKEATMKALGTGWTPHVAFKAIEIVREGRRRPQIELNRDMRRMLPA
                     NLTLHVSLSHTRLLATAFVVAALESEPSLNSSSTREKHHDQIH"
     gene            complement(2707..3261)
                     /locus_tag="PLT86_05910"
     CDS             complement(2707..3261)
                     /locus_tag="PLT86_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36180.1"
                     /translation="MTTAFVVTGAIVLIAAVAVVVWFFTGRRPPRLDPRSERLIESLV
                     QLQRDNPRRATEVFARALVRAGLVWEKDLQTGGTSAQTDRQQPYRDSRRDQPQRQHPS
                     RNEQRVEVRAPAQQAAQRPEVRDTRTGERTESSAASGDKTGSPPQPATHSSRRRRRRR
                     PSGTREQQPGATPQQTTTPPPDQA"
     gene            complement(3556..5709)
                     /locus_tag="PLT86_05915"
     CDS             complement(3556..5709)
                     /locus_tag="PLT86_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT36181.1"
                     /translation="MGKKHKDLRHWLNRGALTVLILVFCVSVSVAQNIGKDGGSGSAT
                     DERRELRIGDRTLGTISTSGGSIRYGLNVNATKTLTIRMDKVLDSAIDPKLTIYDSNG
                     SQVATDDDGGEGLNALISVTLQPGQYVVAAQAVGTSSGDFLLRVEEQQATTLSLGTAV
                     LGEITTSMNEVAYRVNIPRTTNLRIRMNKLLGSTLDPKVTLVDGSGTEIATDDDGGGS
                     LNALLQRSVPSGEYVVRARRVGTSTGKFMLSVYDQDAPLGGASVRVGDSVTEIINDPN
                     VPAVFRLTVNDEFQDCRITVDKTEGSNLDPNLIVTNRSGEIIGSDDDSGEGNGALLRL
                     SLGRGDYQLFVGGYGTTTGAFRLTVAPDRVPSISVGETKRGVLATGGSDLYRFTTPRA
                     REVTISLRKAAAPATSQSVQQFRQIGSIPSGGGFSRFAYTVDSEQPLVVRVDKIGDGS
                     LDPKVTVYDQNGNEVGSDDDSGDGLNARLSRVFAPGVYTIQVSSVNNSSGDFILMVGP
                     DTGGGSTLDPKLIVKNASGSEVASDDDGGEGNDALLTSNLAAGTYQIIAMAYGSTSGA
                     YELTVSEVVPQDMGAISPGQTKTGTLTSGGRHRYSLRVASSAVIAIDVMKAADSSLDP
                     QVVLRGSSGEEIAMNDDGGDGNNARIIRRLEPGSYEIVVRGYQATAGGYTLMVESFAD
                     TRTPAPQREQMEDSPSKGGREGSGSEPEGFGKGAG"
     gene            complement(5788..7284)
                     /locus_tag="PLT86_05920"
     CDS             complement(5788..7284)
                     /locus_tag="PLT86_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36182.1"
                     /translation="MKTRAFFVLRERGILTTNTSQAVFWLSRDGEPTMKLADWSRAVI
                     VFMTLAGGMGLFALAGKAWWRHAASDDAHPGYFVDLAYTTGHAPEDSVSFYAGAAVGN
                     ITPTVPDSFSDRNGDGRFSIGIDTWVDKNRNRRFDAVWLAGEANARPAGGVQDSLYAR
                     AIVFGDARHRIAICAVDNFGIFYDDVIAIRELVAKLLAVRNDSLEYVAVIATGTMCAP
                     DMMGFWGRSEEETGRDPAYAEQLRRTVAETVLRAATGARAAMLEGWQRNTNPAEASIV
                     FRANNGTPIALLSVGAERRRTGGKAGVMISAENDHDFAEQGSPERSGGLAPVHVVLPG
                     IPAGTHSTAAADSPGENAPMRENRCAFSIRAQTVLAPVGMGSIRTAANLGLIDRGFVD
                     GKLRTETAALCLAGIQLVLVPGTIRDPEAVYLEDSPIRGYGKRFLVTRSNDFLGDLDV
                     PSRDTTHSLLPRISIGLEGRRTIMQEMSLLAKDDLFRYPQLHNRCDVR"
     gene            complement(7320..8399)
                     /locus_tag="PLT86_05925"
     CDS             complement(7320..8399)
                     /locus_tag="PLT86_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36183.1"
                     /translation="MDYEPVKFGVVGLGMGQHHCKAIAAANGAELVAVCDIDMERTNK
                     MVQTYGCKGYSSYAEFLNHPGMEAVCICVPSGMHADFGIQAIQTGKHLVVEKPPDVKA
                     AKVQALIDAAAERGVKMEQIFQQRTSPLNRRIKDVIDSGRLGKLVGINALLPWYRAQS
                     YYEDVAHGRWKGTWALDGGGSLANQGVHTMDIVLYFFGRVKSVFGKFGVFTHDIEAED
                     HTVAILKFENGAMGTIMTTTCAYRGTDQILVLFGSRGQIRQSTYLEAWQIMHDDDPKR
                     EKEKAEEQEMLALYGPREQRGETTANDPWALSSDGHQAHIEDLVRAIRTNTDTMSSPA
                     HAKHDVEVIEAIFESGRTGKEVFLS"
     gene            complement(8531..9499)
                     /locus_tag="PLT86_05930"
     CDS             complement(8531..9499)
                     /locus_tag="PLT86_05930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT36184.1"
                     /translation="MQYRDLGKTGLRVSEIGFGAWAIGGGFEIGGKGIGYGATEDETS
                     IRAIHRALDLGVNLYDTADAYGAGHSESLLGKALQGRWSSCYVATKVGNERRDPLPGR
                     KNFTREYILSACERSLKRLRKDVIDLYQLHNPPPEVGDGDEVFETLSILREQGKIRFV
                     GVSVTTPAEGISYIRRGVVDCLQIYYNAQTREAETELFPLCAREKIGTLIRAPLSSGI
                     LAAKFTKDTVFPPDDHRGNWLKGELLARAVAEAEALRKAAAPLPPAEAALRFILNQPA
                     VTCIIVGAKTPEQVERNCAASDGKGLATPVENAIRAVLPGDFPRQT"
     gene            complement(9636..10778)
                     /locus_tag="PLT86_05935"
     CDS             complement(9636..10778)
                     /locus_tag="PLT86_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36185.1"
                     /translation="MRLATQLGIGLLVAGALAAHTARAASVAKPYGFILGMGAYNHGN
                     AYPSDLAFIAQSSASLPGSFLITGRHTRLGMRVQPDSTVPATGVVEVDFWGLRGSTAG
                     GAAQQTSIRLRLAYVRLQPNPRTTLTFGQDWLAFAPANPGVPGLLTTCGNLWVRMPQA
                     RIDYSVAPFLFQFALVRPFVGDMAATPVEQTDPLGSGERSKLPFVQARVAYTLAPGMT
                     FGLSGHMGKQDWNDGTGTINSLAGAFDYDVRRGPFRIVGEFASGQSLGGINALTKFHQ
                     DPATGKRYAETGYMGWFEATYKFSPKWMASVAAGQEKADWNATHTAMLTRQTMDRNRA
                     GYVNVVWQPWAPFRVITQLDVIRSHYIAPAVEHKVEVLSVGAMLIF"
     gene            complement(10824..11750)
                     /locus_tag="PLT86_05940"
     CDS             complement(10824..11750)
                     /locus_tag="PLT86_05940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOT36186.1"
                     /translation="MVLVIGLNPAIDRILIVPNFAVGATLKAESVQTLAAGKAMNVAA
                     VLRLLGVRVGYAGFVGREEAAFFRRRLPDVECWMVEVDGRTRTDTTLIDPVGHTETHV
                     REPGFAVQTEDMERFLDAFTGVIVGVEFVVMSGSLPPGAPEGLYAELVGLCHARHIRT
                     LLDTSGTPLAMGAAARPSFLKPNTEELAELTGGHAGSAAEAALWGRTLLQGNTQAVAV
                     TRGAEGAVLVDRETAIQGNASVPSVVNTVGCGDAFVAGWVAKLVSGSPLTEQLGEALA
                     VAGANAMTHGAGVIERRNLDLMRDRVTISDVW"
     gene            12097..13128
                     /locus_tag="PLT86_05945"
     CDS             12097..13128
                     /locus_tag="PLT86_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT36187.1"
                     /translation="MKQGLILGCGSVVVDVLKLVPNFKVGDKTKIIEQDGRSKKLIAG
                     GVTLNNLVWSRMLGARSGVFGRLGGDEYGKFLLKEMDRFGVAHDQLHVIEGEEAAYTD
                     FFLDRQPERTLFMFFGTTATTTPEFIRTHCADAIRNAAYVVTEISQLPLDTVLEIEKI
                     AHEANVPVAVDLDIAPSNSIMQYIGTAAQYEKVARGATVLKPCDAAATELAGTDDLEK
                     AIQKIHRDFGNHRLVAITAAAKGSLLYDGKNVHWENVLDLSKVGRKLVDPCGAGDAYF
                     GGLNAALNLLGFDAPLADIGTIANATAGICCSEYGAFPVDPETPRAAIKNLIAANRGG
                     ATADRLIPA"
     gene            13188..14318
                     /locus_tag="PLT86_05950"
     CDS             13188..14318
                     /locus_tag="PLT86_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT36188.1"
                     /translation="MKYKASTVQAFLQRIVESLDAQIQHIETASPQTFYGNVFSEIPE
                     ATYWVVTGKGRVRHIADKISASLASTGTPSYFVDAANEAHGLPLLSPSSRVLLLWRRY
                     QPVADLTRLLEAIPASVAVDIVTDATDLPVNRSANLFRIVGVESSALEVPNDIVALAM
                     GDSCVAGLESARKFTELDFLKDHPKGNLGKTIAAAQSAREISYLPPPVQAFAALKEER
                     EGVRNLLEGLDAAAVTEWLDVFSRPWERIITTGMGKPGYCMRYLAFLLNELGRSAFYL
                     HPAEGVHGDLGRIGERSLVIAYSHSGETDEVLNILPRMKERGATLLALTNNTASTMAK
                     AADAALKAGAPEVDPIRKAPTGSTTAALSLATALVCSDRPAR"
     gene            complement(14339..15472)
                     /locus_tag="PLT86_05955"
     CDS             complement(14339..15472)
                     /locus_tag="PLT86_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36189.1"
                     /translation="MTPKQRVFTALRHEEPDFVPIGEFAIDYAAFEAVLGRPTFWRGH
                     FKTMKALWEGRRDEVVESMIRDVIEFYHKTDQDIIPVSLVPSRNKEIIPWEEIAPMTF
                     RDPRGDVYRVSEKSQWLMRIKYAEPAREYSVEDFPVPTEFTEPDESEWELVRAVVKEF
                     GATHFILARSGDGTFPLIGGMERGLILIAEHPDVAEAAAKAAVARTMYMDGLWAKEGV
                     DGLCPGEDYADSRGPLVSPRVIRERFFPSMAAHVAHAHGLGLPVLKHACGNNWKIMDM
                     LVEAGYDAYQSIQESATMDIIRLKREYGDKITLWGGVGTETLIAGSTEDVRNEVRRAV
                     RHCAPGGGFLLGSSHSILIGAQPDNYLAMLDEARKVGRYPVTI"
BASE COUNT         3103 a         4812 c         4418 g         3304 t
ORIGIN      
        1 acacgacacc gaggcggggg aaaacctctt gtggacggcg ttgcggacgg atgcggcgga
       61 ttgcgcgctt tcggacgcaa gtacgttgac aacatggccg ccgccaccct gggtgcgcgc
      121 agttgccttc ttggcgcgga tgaacccgcg ttttgcgctc ttgcaccggt catccgtcaa
      181 actttccgat ctgccggctg caccgcggtt gtccacgtcc agcggtcttg cgagggtgca
      241 attcgacaac gcgaaaccgg gagcggaatg ggtggagctt tcgggagacc ttccgacgcg
      301 tctgcacaag ttgcatctcg ggcatgcaga tggagtcata gaacccgcga gcgatctgtt
      361 gactcttggc atcgcggagg atgcaatcga gttcctgtcc actgatatgc ttcttccggc
      421 gccggggcag ggtgtgtgga tcgtcttctc gctctccgac agagtcgacg tggaacgggt
      481 gttccggcac ctcgacgacc ggaaagcggg gatctgcgcg aaagccgaac gcacgttcac
      541 cgcgcaactg gatgctccgg ccaattgtct tgttggcgcg cgagcgtggg tacaaacgaa
      601 gcgcctgacg ctcgacgcgt gcattatgcc ggcagaaggg ggtgttgttc agcgcctgag
      661 tatctccggg gcgcccgaac agccagaaga gctggttgcg gggctggtcg atatgttccg
      721 ccaccagagt gtcctgcgtc agtgtcgcgg aaaggcagac agggggtgag catgcggatt
      781 cttgtgctga acggcccgaa cctgaacatg ctcggcattc gcgaaccggc agtgtacggc
      841 agccaatctc ttgccggcgt tgaggcggaa cttacttcgt tggcatccaa actcggcgtc
      901 gaggtggagt gtcatcaaag caaccacgaa ggggctctga ttgacatgat ccacgccgcg
      961 ttcgatcgct ttcagggcat cctgatcaac ccgggcgcct tgacgcatta cagttacgcg
     1021 ctgcgcgatg cgatcgcctc cgtcgggctt cctgcagtgg aggttcacct ttcgaacatc
     1081 catgctcgcg agccttttcg tcatcagtcg gtgattgcgg cggtatgtgt cggtcagatc
     1141 tcggggttcg gggttggaag ctaccaactc ggattgagag cgttggcgtc gtacttgcag
     1201 aagactcagg aaaggacccg caaagcgtga gccaccgcat cggctggtgc cgtttctgcg
     1261 gatgagtaca tttcagatat cctcgacttt ctccccggat agctcaacag gatagagcaa
     1321 gtgccttcta agcactaggt cgcaggttcg agtcctgctc cggggatacc caaaaaaagc
     1381 ccgcggactg gtgtccgcgg gctgcgttat ttctgggaat cgggttaccc agaacgccgt
     1441 ccgaaaatga aatccatctg gagaccgatt acgtaaatag aagcctccca gtggtgcttg
     1501 taacaaccga cttccacaga agcggggcca tcatagccta cctctttgaa ggccttcatc
     1561 acggcgggcc agtcgacgtc gccttcgagg aggccgacga atcctccaaa cccgcctacg
     1621 cccgtgcgga agtccttgaa atgaaccttc ttgatccgct ttccgagaat tcggatccac
     1681 tgctccgggt aaccgtacag caacatgttt ccaacgtcca ggtaaacggc cagaaactcg
     1741 ctgttgaagc tgtccacata atcccggagc tctatcggac tcaaatggaa cttgttccac
     1801 acgttttcaa cacagattgc gaccttcgcc ttctccgcgt acggaaccag tttcttcagt
     1861 tcatcctgag tctttttgta aacgacatcg tacgggactt ccggagtagt cacgcccgga
     1921 accacaagca caccgtctgt tcccagccac ttcgcgacgt caacgccttt cttgatcgaa
     1981 tcggctcccg ctttcacgat cttcttgtcc ggagatgaca gagggtattt ccaacccgtg
     2041 ccaatggcca gcgaagcaat tttcaatccg gccccctcaa cgaacttccg gacctctttg
     2101 acctccttct ccgtgctctt ccaggtgatc ggaccggtgt cttcattgag gcacagctcg
     2161 atagcttcga accccgcttt cttcgccagc ggtacgttgg tcttcaggtc gttggaatcc
     2221 cgaaacgacc acaggttaat ggatttgatc atggtgcttc tcccgggttg aggacgaatt
     2281 gagactgggt tctgattcca gagccgccac cacaaacgcg gtggcgagaa gacgcgtatg
     2341 agaaagcgat acgtgcaagg taaggttcgc cggcaacatc cgtctcatat cgcggttcag
     2401 ttcaatctgc ggtcgccggc ggccttctct gacgatttcg atggccttga aggccacatg
     2461 gggtgtccaa ccggtgccaa gtgccttcat ggtcgcttct tttgcagcaa accgggccgc
     2521 caggtgctcc gccggaagcg gccgttctcg gcaatactcc agctcgcgtt gcgtgtacac
     2581 acgcttcaga aagcgatccc cccatcggtt ggcggcacgg tcgatacgtg caacatcgac
     2641 gacgtcgacg ccgatgccaa aaatcctgct cacgacagcc tcaacaatcc tgcacttcgc
     2701 gttctatcac gcctgatcgg gcggcggcgt agtcgtttgt tgcggggttg caccgggttg
     2761 ttgctctctt gtccccgatg gacgacggcg tctgcggcgg cgcgatgaat gcgttgcagg
     2821 ctgtggcgga ctgcccgttt tgtctccgct cgccgccgat gattcggttc tttcaccggt
     2881 tcttgtgtcg cgaacctccg gccgttgcgc tgcctgttgc gcgggagcac ggacctcaac
     2941 acgctgttcg ttgcgggaag ggtgttgccg ctgaggttgg tcgcgtcgcg agtctcgata
     3001 gggttgttga cgatcagtct gcgcggaagt cccccctgtt tgcaggtcct tctcccacac
     3061 cagaccggca cggaccagcg cccgggcgaa tacttccgtc gcacgccggg ggttatcccg
     3121 ctgaagttgg acaagcgatt cgatgaggcg ttcgcttcgc gggtcaaggc ggggaggacg
     3181 ccggccggtg aaaaaccaga caaccaccgc gaccgccgcg atcagaacga tcgccccggt
     3241 taccacaaaa gctgtagtca ttcaccacct cgggttttcg ttaccatacg tgttcctcaa
     3301 ttcggaagtc gttctgagtt cggcgcaccg gaacccggcg gcgtccagaa aaggcgtgca
     3361 agccagaact cctgtggagg actggctatc tccagggtac ggacggattg ccaaaggcaa
     3421 aggcttcgaa atggatgcac aatcagaaag tacggcaata cgaacctccc ctcaagcgcg
     3481 gaggttcacc cgcacaactg aatacaaaga aaaacggggc gcttttgggc gccccgcttc
     3541 ttccgggttc acctatcagc ccgcgccctt accgaaacct tccggttcgc ttccgcttcc
     3601 ctcgcgtcca cctttggagg gagagtcttc catctgctca cgttgcgggg cgggtgtgcg
     3661 tgtgtcggca aacgattcaa ccatcagggt gtatccaccc gctgttgcct ggtatccgcg
     3721 cacgacgatt tcgtacgatc ccggttccaa tcggcgaatg atgcgggcat tgttgccgtc
     3781 accaccgtcg tcattcatcg cgatctcttc cccggacgac ccccgaagaa ctacctgagg
     3841 atccagcgag gaatcggcag ccttcatcac atcgatcgca atgacggcgg atgaggctac
     3901 ccgaagggag taccggtgcc ttccccctga agtcagagtc cctgtttttg tctgtcccgg
     3961 tgaaatcgcg cccatgtcct gcggcactac ttccgaaacc gtcaattcat acgcaccgga
     4021 agtcgatcca taggccatcg cgatgatctg gtacgttccc gccgccaggt tcgaggtgag
     4081 tagagcgtca ttgccctcgc caccgtcatc gtctgaagcg acttcacttc ccgaggcgtt
     4141 cttgacgatc agtttcgggt cgagcgtgct gcctccaccg gtgtccggcc ctaccatcag
     4201 tatgaaatcg ccgctgctgt tgttcacgga gctgacctgg atggtgtaca cgccgggagc
     4261 aaacactcgc gaaaggcgtg cgttcaggcc gtcgccgctg tcgtcatcag aacccacctc
     4321 gtttccgttc tggtcgtaca cggtcacctt cgggtccagc gaaccgtccc caattttgtc
     4381 tacacgtacg acgagcggct gctccgaatc cacggtgtac gcaaacctgc tgaaaccccc
     4441 gccggacggg atcgatccga tctgccggaa ttgctgcaca ctttggctgg tcgccggtgc
     4501 cgcagccttt cgaagtgaga tggttacctc acgggcgcgc ggtgtggtga acctgtatag
     4561 atcactgcct cccgtcgcca gaacgccccg cttcgtttct ccgaccgaga ttgagggaac
     4621 ccggtcagga gccaccgtga gacggaaggc gccggtggtt gttccgtaac cgccgacaaa
     4681 cagctggtaa tcacctcgcc cgagggacaa tcgcaacaat gcgccgttgc cctccccaga
     4741 gtcgtcgtcg cttccaatga tctcaccgct gcggttcgtc acgatcagat tcggatcgag
     4801 gttgctccct tcggttttgt ccaccgtgat acgacaatcc tggaactcgt cgttcacggt
     4861 cagacggaaa actgctggca cattcgggtc attgatgatc tccgtgacgg aatcaccgac
     4921 acgcacactg gctccaccca gaggagcatc ctgatcatag accgagagca tgaatttccc
     4981 ggtgctggtg cccactctgc gggcccgaac gacgtactca ccgctcggaa ccgacctctg
     5041 gaggagggca ttcagacttc cacctccgtc atcatccgtt gcgatttccg tcccgctgcc
     5101 atcaacaagc gtcactttgg gatcgagtgt agaaccgagc agtttgttca tccggatacg
     5161 cagattcgtg gttcgcggga tgttcacgcg gtaggcaacc tcgttcatgc tggtcgtgat
     5221 ttcgccaagc actgcggttc cgagagacag ggtggtagcc tgctgttcct caacacggag
     5281 aagaaaatct ccgcttgatg ttcccactgc ctgagctgcc accacgtact gtccgggttg
     5341 cagcgtgacc gagatcagcg cgttcagccc ctccccaccg tcgtcgtcag tcgcgacttg
     5401 cgaaccattg gagtcgtaaa tggtgagttt tgggtcgatt gcggaatcca ggaccttgtc
     5461 catccgaatc gtcagggttt ttgtggcgtt cacgttgagt ccgtaacgga tggaaccccc
     5521 gcttgtcgaa atcgtaccca gcgtcctgtc tccaatccgc agctcgcgcc tttcatccgt
     5581 ggcggaaccc gagccgccgt cctttccgat gttctgcgcg acggaaactg aaacacagaa
     5641 caccagaatc agcacggtca acgccccccg gttcagccag tgacgcaagt ctttgtgttt
     5701 ctttcccatt tcgttcccct cccgattgtg tcttggcggt gtgaaacctg gtatgatcga
     5761 tggcaacctc tctggtgatc aagcgcgtca acgaacatca catctgttgt gcaattgtgg
     5821 ataacgaaac aagtcgtctt tcgcaaggag gctcatctcc tgcatgatgg tgcgtcttcc
     5881 ttccaggccg atcgaaatcc gcggaaggag gctatgcgtc gtgtcccgtg agggcacatc
     5941 aaggtcacca agaaagtcgt tcgatcgcgt tacgagaaac cgtttcccgt acccccggat
     6001 gggtgaatcc tccagataga ccgcctccgg gtctcgtatt gttcccggaa cgagaacgag
     6061 ctggatccct gcgaggcaca gagccgcagt ctccgttcgg agtttcccgt cgacgaaccc
     6121 gcggtcgata agcccaaggt tggccgcagt tcgaatcgat cccatgccga ccggagccag
     6181 aaccgtctgc gcgcgaatgg aaaaggcaca cctgttctcc cgcatgggtg cgttttctcc
     6241 ggggctgtcc gcggcggcgg tgctgtgggt tcctgcggga atacccggca gcacaacgtg
     6301 caccggcgca aggcccccgg atctctcagg agagccctgc tccgcgaaat cgtggtcgtt
     6361 ttctgcggag atcattacgc ctgccttgcc gcctgtgcgc cgccgttcgg ctccgacaga
     6421 aagtagtgca attggcgtac cgttgttcgc gcggaacacg atcgatgcct ccgcggggtt
     6481 cgtgttccgt tgccatccct cgagcattgc ggcgcgggca ccggttgccg cacggaggac
     6541 ggtttccgcg acggtgcgcc ggagctgttc cgcgtacgcg ggatcccttc cggtctcttc
     6601 ttccgaccgt ccccagaagc ccatcatgtc gggcgcgcac atcgttccgg tagctattac
     6661 cgcgacatat tcgagagaat cgttccttac tgcaagaagc ttcgcaacga gttcgcggat
     6721 ggcgatcacg tcatcgtaga agatgccgaa attgtccacc gcgcaaatcg cgatgcgatg
     6781 ccgtgcgtcc ccgaaaacga ttgcgcgggc atacaggctg tcctgaacgc ctcctgccgg
     6841 acgagcattc gcttcgccgg ccaaccacac tgcgtcaaag cgcctgttgc ggtttttgtc
     6901 tacccatgta tcgatcccga tactgaatcg cccgtctcca ttccggtcgg agaaactatc
     6961 cggcacggtc ggggtgatat ttcccacagc cgccccggcg tagaaagaaa ccgaatcctc
     7021 gggcgcgtgc cccgttgtat acgccaagtc aacgaagtaa cccggatggg catcatcgct
     7081 cgccgcatgg cgccaccagg cctttcccgc cagagcgaaa aggcccattc ccccagcgag
     7141 ggtcatgaac acgatgacgg ccctgctcca atctgccagt ttcatcgtcg gttcgccatc
     7201 ccgtgaaagc cagaacacgg cctgagaggt gttggttgtc agaattcctc gctcgcgcaa
     7261 aacgaaaaag gcgcgcgttt tcacgcgcgc cttcgatcgt cacggagccg agtccatcat
     7321 cacgaaagaa acacctcttt gcccgtacgg ccgctctcga atatcgcctc aatcacttcc
     7381 acgtcatgtt ttgcgtgagc gggggagctc atcgtgtcgg tgtttgtccg aatggctcgc
     7441 accagatcct cgatgtgcgc ctggtgccca tccgaactca gtgcccaggg atcgttggcc
     7501 gtggtttcac ctctctgctc gcggggacca tacagcgcga gcatctcctg ttcctcggcc
     7561 ttctcctttt cgcgtttggg gtcgtcgtcg tgcatgatct gccacgcttc gaggtacgtg
     7621 ctctgccgta tctgtccccg tgagccgaac aataccagaa tctggtctgt tccgcggtac
     7681 gcgcaggtag tagtcatgat ggttcccatg gcgccatttt cgaacttcag gatcgccaca
     7741 gtgtggtctt ccgcttcgat gtcgtgcgtg aacacgccga atttcccgaa gacagacttc
     7801 acccttccga agaaatacag cacgatatcc atcgtgtgaa cgccctggtt ggcaagcgaa
     7861 ccccctccgt ccagcgccca ggttcccttc caacgaccgt gcgcaacgtc ctcataatag
     7921 ctctgggcac ggtaccaggg cagcaaagcg ttgattccga ccagtttccc gagtcgtcca
     7981 ctgtcgatga catccttgat ccgtctattc aaggggctcg tgcgctgctg gaagatctgt
     8041 tccattttta cgccgcgttc tgccgccgcg tcgatcaggg cttgaacctt tgcagccttg
     8101 acgtccggtg gtttctccac gacgagatgc ttgccggtct gaatcgcctg tatcccgaaa
     8161 tcggcgtgca taccactcgg aacgcagatg cacactgctt ccatacccgg gtggttcagg
     8221 aactcggcgt acgaggaata ccctttgcag ccgtacgtct gaaccatttt gtttgtgcgc
     8281 tccatgtcga tgtcgcatac cgcaaccagt tccgcgccat tggccgccgc gattgccttg
     8341 cagtgatgct gccccattcc caggccgacg acaccgaatt tcacgggttc gtaatccatc
     8401 gttcacagct ccatcatctg tgcgggaaaa caaaacgagg tcgccccagc tttctgtttc
     8461 tcgaaaggtt tccaaaaggg cgttatcgta ttgattatac gttccccggc ctgctgtccc
     8521 taaggggttt tcaggtctgc cgcggaaaat cgcctggcag gacagcgcgg attgcgttct
     8581 ccaccggggt tgcaaggccc ttcccgtctg acgctgcgca gttgcgttcc acctgctccg
     8641 gtgtcttggc gcccactatg atgcacgtga ctgctggctg gttcagaatg aagcgcaagg
     8701 cggcttccgc gggcggcaaa ggtgctgcag cctttcgcag tgcttccgct tcggcaaccg
     8761 cgcgggcgag caactcgcct ttgagccagt tgccgcggtg atcgtcagga gggaacaccg
     8821 tgtccttcgt gaacttcgcc gccagtattc ctgagctgag aggggcgcgg ataagggtac
     8881 cgatcttctc ccttgcgcaa agagggaaaa gctcggtctc cgcttcacgc gtctgcgcgt
     8941 tgtaataaat ctgaaggcag tctacgacgc ctcgcctgat gtacgaaatg ccctccgccg
     9001 gggtggtgac ggaaacgccg acgaaacgga tctttccctg ctcacgaaga atcgagagtg
     9061 tttcgaatac ttcgtcccca tctccaacct caggcggcgg gttgtgaagc tggtaaagat
     9121 caattacgtc cttgcggagc cgcttgaggc tgcgttcgca tgccgacagg atgtactcgc
     9181 gggtaaaatt cttgcgcccg ggaagaggat cgcgccgctc gttgccaacc tttgtggcaa
     9241 cgtaacacga agaccatctc ccctgaagag ctttgccaag aagtgattcg ctgtgtcccg
     9301 ccccgtatgc gtcagcagtg tcatacagat tcacacccag atcgagcgcc cgatggattg
     9361 ctctgatcga cgtctcatct tccgtcgctc cgtagccgat accttttcca ccgatctcaa
     9421 aacctccgcc aatcgcccag gcaccgaatc caatttcaga gacgcggagt cccgttttgc
     9481 caaggtcccg atattgcatt tggaacaccc tcctgtaaga acagggaacc cgatgccgtg
     9541 cccgaaggac cgccacaggg ttcttccatc aacagccagc gcgccgcccg caccaactgg
     9601 tgcgagacgg cgcgcccggc taccagaagc aacaactaga aaatcagcat ggctcccacg
     9661 ctgagcactt ccactttgtg ttccactgcc ggggcaatgt aatgcgaccg aatcacgtcc
     9721 agctgggtga tgacacggaa cggcgcccac ggctgccaca caacattcac gtagcccgcc
     9781 cggttccggt ccattgtctg ccgggtcagc atcgcggtat gcgtggcgtt ccagtcggct
     9841 ttttcctggc cggctgcaac actggccatc cattttggcg aaaacttgta cgtcgcctca
     9901 aaccagccca tgtacccggt ctcggcataa cgcttcccag tggcggggtc ctgatggaac
     9961 ttcgtcaaag cattgatgcc gccgaggctc tgcccagagg caaattcccc gacgattctg
    10021 aagggtccgc gccgaacgtc atagtcgaac gcgcctgcca gggagttgat agtgcccgtt
    10081 ccgtcgttcc aatcctgttt ccccatgtgt cctgaaaggc caaaggtcat ccctggtgcg
    10141 agagtgtacg caacgcgtgc ctgcacgaat ggcaacttcg aacgttcgcc gctgcccaac
    10201 gggtcagttt gctcaaccgg cgtggctgcc atgtccccca caaacggacg gacgagcgcg
    10261 aattgaaaca ggaatggcgc gacagagtaa tcaattcgcg cttgaggcat gcgcacccag
    10321 aggttcccgc acgtagtaag taagccggga actccggggt tcgccggcgc gaacgcgagc
    10381 caatcctgtc caaacgtaag ggttgtgcgg ggattgggct gcaatctcac gtacgccaga
    10441 cggagccgga tgctggtctg ttgcgcggct ccgcccgccg ttgaccctcg aagcccccag
    10501 aagtccacct ccacgacccc ggtcgcgggg actgtgctgt cgggttgcac acgcattccg
    10561 aggcgcgtgt ggcgccccgt tatcagaaag cttcccggca gtgacgccga gctctgagca
    10621 atgaacgcaa ggtcactcgg atacgcgttg ccgtggttgt acgcacccat tccgagaatg
    10681 aatccgtacg gtttggccac cgatgctgcg cgtgcggtgt gcgccgccag tgctccggcc
    10741 acgagaagcc cgattcccag ttgtgttgcc agtctcatca ggtcgtcccc cgatctattt
    10801 gagaggtggt tgaagaaaac cgttcaccag acgtccgaaa tggtgacacg gtcgcgcatg
    10861 agatccagat ttctgcgttc gataactcct gctccgtgcg tcatagcatt tgcccccgct
    10921 acggcaagtg cttcacccag ttgctccgtg agcggagaac ccgaaacgag tttcgcgacc
    10981 catcctgcaa cgaatgcgtc gccgcatccg acggtgttga caacgctcgg gaccgacgcg
    11041 tttccctgga tcgccgtttc ccgatccacc agcaccgcac cttccgcccc tcgagtgacc
    11101 gcgaccgcct gcgtgttccc ctgcagcagc gttctccccc acagggcggc ttccgctgcg
    11161 ctccctgcgt gcccgccggt cagctcggcg agttcctccg tattcggttt cagaaaactc
    11221 ggcctggcgg ctgctcccat ggccaacggc gttcccgacg tgtcgagtaa tgtccgaatg
    11281 tgtctcgcgt gacacaaccc caccaattca gcgtacaacc cctcaggggc ccccggaggg
    11341 aggctccccg acatcaccac gaactccacg ccaacgatga ctcccgtgaa cgcgtcaagg
    11401 aaccgttcca tgtcttcggt ctggactgca aatcccggct ccctcacgtg agtttccgta
    11461 tgacccaccg ggtcaattag cgtggtgtca gtccgcgtcc gaccgtcaac ttccaccatc
    11521 cagcattcca catcaggcaa gcgtctgcga aagaaagcgg cttcttctct tcccacaaac
    11581 cccgcgtatc ccactcgtac cccgagcagc cgcagaaccg cagcgacgtt catcgccttg
    11641 ccggcggcca gcgtctgcac cgattctgct ttcagagttg ctcccaccgc gaagttcgga
    11701 acgatgagga ttctatcgat ggcgggattc agaccgatta ccagaaccat gggaaaaccg
    11761 gacgcgacgg gttgttaaag cggatacgtg agagaaaggt aggacgacat tctgcgcggg
    11821 caaagcgcgg catgcatggt ttcatccgat ccccgcattc gagtatcatt acaaagaacg
    11881 cgatgactgg caggaacgcc ggcgttttcc cgctgcaggt gccagaccct cgacggcggt
    11941 ctgtatccag tccggtacaa agccggggac gaccggttcg cccggatttc gtggtctcct
    12001 gagtcacccg aacggtggag aactattccc tgcgccggcc ccatcacggc cggttcggca
    12061 cgtaggaacg gcaaccagaa accaacgagg gaaacgatga aacagggctt gattctcggc
    12121 tgcggaagtg tggtcgttga cgtgctcaaa ctcgttccga atttcaaagt cggcgacaag
    12181 acgaaaatca tcgaacagga tggtcgctcg aagaagctca tcgccggcgg cgtgacgctc
    12241 aacaacctcg tctggtcacg catgctgggc gcccggtcgg gagtgttcgg gcggctcggc
    12301 ggggacgaat acgggaaatt cctgttgaaa gaaatggatc ggttcggcgt tgcacacgat
    12361 cagcttcacg tgatcgaagg cgaagaagcc gcctacactg atttcttcct cgaccggcaa
    12421 ccggaacgca cccttttcat gttcttcgga accacggcga ccaccacacc tgagttcatt
    12481 cgcacgcatt gcgcagatgc catacgtaat gcggcctacg tcgtaacgga gatctcccag
    12541 cttccgcttg acacagtact ggaaatcgag aaaatcgcgc acgaggcaaa tgtgccggtc
    12601 gcggttgatc tggatatcgc cccgagcaat tccatcatgc agtacatcgg aaccgcggcc
    12661 cagtacgaaa aagtggcacg cggcgctacg gttctcaaac cgtgcgacgc cgctgcaact
    12721 gaactggcgg gtaccgatga cctcgagaag gcgattcaga agatccatcg cgactttggc
    12781 aaccacagac tggtagccat tacggccgcg gcgaaaggaa gtctgctgta cgacgggaaa
    12841 aacgtccact gggaaaacgt gctcgatctc tcgaaagtcg gacgcaaact cgtcgacccg
    12901 tgcggtgcag gagacgcgta ttttggcggt ctcaacgctg cgctcaacct gcttgggttt
    12961 gacgcccccc tcgcagatat cgggacaatc gcgaacgcca ctgcgggcat ctgctgttct
    13021 gaatacggcg cattcccggt tgacccagaa acgccgcggg cggcgatcaa aaaccttatc
    13081 gccgccaaca gagggggggc aacggcagac cgcctgattc ccgcgtgacg cactgcccgc
    13141 ggggtgagcc gaatcacctc gcgggctcat tcttttgagg aaatcccgtg aaatacaaag
    13201 cttcaaccgt acaggcgttc cttcaacgca ttgtagaatc cctcgatgcg cagatacagc
    13261 acatcgagac tgcctcacca cagacattct acggcaacgt gttctccgaa atcccggaag
    13321 cgacatactg ggttgtaacg ggaaaagggc gcgtgcggca cattgcagac aaaatctccg
    13381 cttctctcgc gagcaccgga acgccctcgt acttcgtgga tgcagcgaac gaagcgcacg
    13441 ggttgccctt gctgagccct tccagccgtg ttcttctgct ctggcggcga taccaaccgg
    13501 ttgccgatct gactcggctg ctcgaggcaa tccccgcttc ggttgcggtt gacatcgtaa
    13561 ccgatgcgac tgatctcccc gtgaacaggt ccgcgaatct ttttcgtatt gtcggcgtcg
    13621 aatcgagcgc actcgaagtg ccgaacgaca tcgtcgcgct cgccatgggc gattcgtgtg
    13681 ttgccgggct ggaaagtgcg cggaaattca cggagctgga cttcctcaag gaccatccca
    13741 agggcaacct tgggaagaca atcgctgccg cgcagagcgc gcgcgaaata tcgtacctgc
    13801 cgccaccggt gcaggcgttt gccgcgttga aggaagagcg cgaaggggta cgaaatctgc
    13861 tcgagggact tgatgcagcg gccgtaacgg aatggctgga cgtgttctca cgcccgtggg
    13921 agcgcattat caccacgggc atggggaaac ccggttactg catgcggtat cttgcctttc
    13981 ttctgaacga actgggacgc agtgcgttct acctgcatcc ggctgaaggt gtccacggag
    14041 acctcggcag gattggcgaa cgaagcctcg tcatcgcgta ctcccatagc ggcgaaacgg
    14101 acgaggtatt gaacattctg ccgcggatga aagaacgggg agcaacgctt cttgcgctta
    14161 cgaacaacac cgccagcacg atggcgaagg cggcggacgc ggcgctgaag gcgggagcac
    14221 cggaggtcga tccgatacgg aaggcgccca ccggttccac cacggcggcg ctgtcgctcg
    14281 cgacggccct cgtgtgttcc gatagaccgg cgaggtagca gggcacagca cggcggaatc
    14341 agatcgtaac aggatagcgg ccgactttcc tcgcctcgtc gagcatcgcc aggtagttgt
    14401 ccggctgtgc tccaatcaaa atggagtggc tggaaccgag caggaagcct ccgccgggtg
    14461 cgcagtgcct tactgcgcgg cgaacttcat tgcgcacatc ctccgttgaa ccggcaatca
    14521 atgtctccgt gcctacgcct ccccacaggg tgattttgtc cccgtattcc cgtttcaggc
    14581 ggatgatatc catcgttgcc gattcctgga tggattggta cgcgtcgtac cccgcctcca
    14641 cgagcatgtc catgattttc cagttgttgc cgcacgcatg tttgagcacg ggcagaccaa
    14701 ggccgtgggc gtgagcaacg tgcgcggcca ttgaagggaa gaagcgctcc ctgatcacgc
    14761 gcggcgacac gagggggccg cgggaatcag cgtagtcctc accgggacac aatccgtcaa
    14821 cgccttcctt tgcccagaga ccgtccatgt acatcgtgcg ggcaacagcc gcctttgcgg
    14881 cagcctccgc aacatccggg tgctcggcaa tcaggatgag cccgcgttcc atcccgccga
    14941 taagtgggaa cgtaccgtcc ccgcttcgcg ctagaataaa atgggtggcg ccgaattcct
    15001 tcactactgc acggacaagt tcccactcgg actcgtcagg ctccgtgaac tccgtcggaa
    15061 cggggaaatc ttcaaccgag tactcacgcg ctggttcggc atatttgatc cgcatcagcc
    15121 actggctttt ttccgagact ctgtagacgt cgcccctggg gtcccggaag gtcatgggcg
    15181 cgatttcctc ccagggtata atttctttgt tcctgcttgg aacgaggctg accggtatga
    15241 tgtcctggtc ggttttgtgg tagaattcaa tgacgtcgcg gatcatgctc tccacgactt
    15301 cgtcgcgcct tccttcccag agggccttca ttgtcttgaa atgcccgcgc cagaacgttg
    15361 gtcttccgag cacggcctca aaggcggcgt agtcaatggc gaattccccg atggggacga
    15421 agtcgggttc ttcatggcgt agcgccgtga aaacacgttg ttttggtgtc atccgttcat
    15481 tccaggcaac gagtgtggtt ggcaaaacgg gaaaggcacc acacaatcta ccaacgtgaa
    15541 ccatcgagcg gaacggcagc ccctgggcgc aggagccgtt ctttcgaaag gcgtcggcag
    15601 tgcgtttgcc gttctcggta cgcgggttgc ttctttt
//
LOCUS       DAOSOO010000058        15472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3835_length_15472_cov_5.517481, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000058 DAOSOO010000000
VERSION     DAOSOO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15472
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3835_length_15472_cov_5.517481"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3508)
                     /locus_tag="PLT86_05960"
     CDS             complement(<1..3508)
                     /locus_tag="PLT86_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT36190.1"
                     /translation="MNVFMLAAVFCMLCGAADETRAAGGEWPMYRQNPHQTGRTDAVS
                     RMREAPVLRSETYLGGPVPDLAGSDETDSSWVFDFDGDGRREQLLFGTLRIRRIDDGA
                     LLWQDNTTGKTFSSALSQVADFLPDSSGLEVVAFTADMGTGEGRGYMFSFHGGISNGR
                     LAWSTPNLGDMYSPEVVAGDFNNDGKMEICLAPHYKIQVLDAATGFTRYVATWDVGRN
                     YGHFAAADVDGDGIRDFVVISDFATHLSVIKNAGSSASLLWKHTYALVDGSERDVIVL
                     PNPHSVGDLDGDGTCEVTLSLFNRPVQDLKWHATAYRGADGTVAMNIPDRYIYGIRDA
                     DNDGIKELFTIECFGTTPPLFGKLQVFNWTATSGAQARLTVEHATFVRTRIGADTTWN
                     TIAAHGDETVLLNDLDGDGVEEFLTLEDTDGDFRGERLRSWQVAAAGISQRMEYVSSA
                     PIAVVSVHGSGAECRIALRNLSTNALVTLDAAGNVLSEDPNDRPKGYRPTPVVSDLDG
                     DGRNEILVTSAGGDILCYDTSGPELAIRWRRPGYAMIEMPAYTLEPLSVLTDDLDGDG
                     KKEVIARNRTQQGFASLIVYNYDGTTRWEHPFPEITDGQVEAAIDNWLSGYFVGDDRI
                     RDIVVQLHARARGSGELLVLNGATGAEVWRKTQVTTATGATSLTMGGYIPAIHDVNGD
                     GIDDIVHIAYTLLAAYDGQTGNPIYPIKNLAYEIFPKWVSYATPTVADFRGTGQHQIF
                     MNSPSYAAGAVTVMEFDGTPRWWYPLENPQGFRSFGAIGDGDGDGVKEIVVGHLDGRV
                     VCYQGTDGAIEWTESVTEGGTWDFVAADIDGDGRDEFITVLQGNTLVAYNGTNDASEP
                     RVQWSLPLGAGGNNPVVADVDNDGIAEILICVRSGHMRIYEAPPRTTGLLKTEPHNGA
                     VNVERNAPIEFVFDELMDSASVASSLSVVPAIPFHCRWEATRLILGTDTPMDSNTTYR
                     VTICAAACNRWGMPLGADASLEFTTGTEVRCARFRGIWPHHRSVEMQATDFIRVDYSR
                     GVEPASVAEAVRVDPPVAGRWQLGDTTASFIPLNFWKAGRYRLTVFPTAHDGSGQNWD
                     VNGDGSAGVGDTLISEFVVAGGVEKRYVFRNLSVILNAQRPDSTFIITIPDRLDSSLV
                     GRIELTPSSVDSADEVWLRVNDG"
     gene            3699..4775
                     /gene="holA"
                     /locus_tag="PLT86_05965"
     CDS             3699..4775
                     /gene="holA"
                     /locus_tag="PLT86_05965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT36191.1"
                     /translation="MQARTRYNAPLTDSADVRDSICGQGAGAYLLLGDNGVDIEETVA
                     GVVAHFTDEATRAFNCDVFHVGEAECTAEAVVNSVRSYPVLAEVRVVIVRGIEGANAA
                     FGNELVLLVENLPPAVVLVMSGEKLDERTKWASVLKKRCTVFTFAVPRNVTPWIRQRA
                     KSRGFTFDDDAAELLSDFVGTNVFRAADEIEKLTLFVQPRKTISAEDVEAVAGIARTD
                     TVYQLNDAIDGGHSERALQIAHRLQEHSMQAATIAGLILQHWMQLRAAADVQQNPDEI
                     PLKELIGENRDFVLKKLTDSARALGRDRIRRGVRLALAAESAMKSSWLADKSVGLDAL
                     ILQLSVPPGGRAPRGHSAYARGKT"
     gene            4864..5199
                     /gene="trxA"
                     /locus_tag="PLT86_05970"
     CDS             4864..5199
                     /gene="trxA"
                     /locus_tag="PLT86_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT36192.1"
                     /translation="MWKMAKPVVVTDATFDQEVLKSDILTVVDFWATWCGPCRMIAPV
                     VEELAAAYDGKVKFAKVDVDENQQHAGQYGIRSIPSLLFFKGGKVVDQVVGAVPKGTL
                     ETKIKAQLG"
     gene            5211..5286
                     /locus_tag="PLT86_05975"
     tRNA            5211..5286
                     /locus_tag="PLT86_05975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5244..5246,aa:Thr,seq:cgt)
     gene            5359..5949
                     /locus_tag="PLT86_05980"
     CDS             5359..5949
                     /locus_tag="PLT86_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT36193.1"
                     /translation="MNVRDFRQPEHLVSEPFLQRWSPRALTGEPITEDELNGLFEAAK
                     WAPSAFNNQPWRFLYAHRGSPEWDVFSDLLVEGNKAWAKNAGALILLVSKRTLDHNGQ
                     PSRTHSFDTGAAWMSFALEASLRGLAAHAMAGFNANKAQQVLGVPEDFAIEVMIAVGR
                     QGPPEILPPDLRERERPNTRKSVSEIARGGVWKDWR"
     gene            complement(5963..6607)
                     /locus_tag="PLT86_05985"
     CDS             complement(5963..6607)
                     /locus_tag="PLT86_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36194.1"
                     /translation="MKPHVSAMCVLMLALVGCSRPLSPDQQRERYREEGWTAYRAGNF
                     TEAETKFRQAAELDSRDGEAQLGVAWSLIRQDRLASALTAIGRISSQDPAYIHATAAG
                     AIIFDAQGQHGEALLAARTVLSQDSLYVFPHDDRITWRTLCYIAGKSTLILAPVADPD
                     LSRTMAFLARVDVEPPVVPDDPTTWYAAGVRYATLMEVLAKRLEGVFYAQVAQR"
     gene            complement(6604..9204)
                     /locus_tag="PLT86_05990"
     CDS             complement(6604..9204)
                     /locus_tag="PLT86_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36195.1"
                     /translation="MRVRTSAYVAVLCAAGFASADAAAPIPCGSHLFTALPESFFRED
                     VSRAKPARNPVVGAKETLWIQNFAAGQLEEVSVTCRAVNRRSVILVADACWSALPSAT
                     AIDPADVTRLSAAFEENAGSPAPVGKGIYDVLTTAFAPVNPVGGDSLVYIALTEIKSN
                     YTGGYIVGYVSPNDRVDPSLYDRSNFRNLVVLDGLSRTTAVQRERTLAHEFLHVIHMG
                     IDRYEERWIDEGIAVLAQVLCGYQGNTGREFFANTDIGLFNDGTATPSLGDYDKSYMI
                     MQYLADQFGVQTIGEIVRSPQHGISGIDAVLASRGFSSRFERDVFPDWTVANLLPPAR
                     NTPGRYAWFDARAAFPGDFGRTAFPCSLRDTLASYGVRYLRAAEQTAQGTITVSSGGV
                     WNIRSVENSEAVTNEPVIRSWFPSGTKVFRDCSLRQPWLVIAPTGMVTVNHVMDLSWQ
                     APGTLNPVAEQREPLGTGTDIYRARIKLIVSNVDPGQDVRVIVSSARTGVLDRDLRVL
                     RSGLKGTCGETLEMIDSSDAPLPADDVIRVTVSDLHSPFGGVLSSEELSWSFGTGEAD
                     LAAPNVVINLLGNPVLPAYVTALVLSDEPLYRNGTIPVSLLLNGTTVVELVPRDSTGM
                     CWVGNVALTSTGAFDFVLRAVDLAGNSPAQPSATYAALTPGGGVARVVSGSGGEPVEF
                     TLAPGTGGGMIVLARVFADGSIWLGPDGARWTIPVRVALPRAWGSRGNALQSVTTGHT
                     EPFSFDPTRGRYWVVLAEPGTYRITNAAGRPVEFSLGQNAPNPFNPSTRIPVSIPARY
                     EGGRLEVFTVEGQLVRSFAVAPGRSSVEWDGLDETGKGVASGIFLARLTAIRDGLPAE
                     TRVRRMVVVR"
     gene            complement(9301..12381)
                     /locus_tag="PLT86_05995"
     CDS             complement(9301..12381)
                     /locus_tag="PLT86_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT36196.1"
                     /translation="MGVGRGAGGRVIAGWIAMIWGVGLLATPASAQWWNEISHFGRNK
                     VNYQAFEWRVIETENFRVYYYQGEGDIARVASQMAEDAYARISRTLDYRLSQPVPLLV
                     YDSHNDFEQTNATWGLIDESTLGFTEAFKNRVVVPFTGSYTQFRHVIHHELVHAVMFD
                     MFYRRQGGLSTASQLWWGPPLWFAEGLAEYLSTDWTTEKNMWVRGATIDGYLRLDGYQ
                     AYTAGYSLLRWIADEFGADKIPAITRRMAITKEPAASFRAVLGVSLDDLQTRWERHLK
                     RFYWPEIERRELTDEMGRRLTDHRKDRNHYNFAPAFSPHGDRIAFLSDRDDYASIYIM
                     SAIDGRILRKVVTGERSSHFEEMHWSRGSLSWSPGGSHLVFAAKHHDLDALYIVSAEN
                     GRIQRMFRWRQFNALFSPSWSPDSLNIVFVGVREGRSDLYLVNTLTGELRQLTDDFYD
                     EIAPRWSPDGTRIAFASDRVLDGEDSNVLPVGFDRNVFVMDVSTGVITQITRAEGEDS
                     SPCWSPDGRRLAFTSDRNGIYNLYVADLDSGTERPLTNVIDGVFQPDWSRDGRKIAVA
                     AFNVAGYDIFTLTDPLAKWQADDPLPPAEFLERRREEAQSDSSRYLERPPVSGLGHWR
                     FSLQPHDSSGNPLTFVSKPYDVHFSPDLVSGLVMFDNINGLMAQTMLRVSDTMGDHQI
                     QIYTHIYRNVEDADFQFAYYNLRKRPNWGFSLFQFHQYYLQSFAATEWTSDRVLGGAV
                     LLNYPFNKFTRLETSLELSDRERANFSYDTYLRTPVRRLPGVRTLLPSFALINDNTLW
                     GVTGPTNGSRSAFQVKFAPEIGFNTYAYTTLIGDYRKYLRVSADYALALRVSSGMSVG
                     RNPGRFLLGGTSYWINFHYTPTYSVTNDSLIPITQFITPLRGSRFGELSGSRYALANI
                     EFRYPLVRELAWGWPLPIVLRNIRGILFLDLGTVWGDGRGFAPFAGNGIKLNYTSAGM
                     TNAPTRGPLAVGGFGFGWRMNLGVAVLRWDIAWPTDLDRTLGGSRQYWSLGADF"
     gene            complement(12412..13014)
                     /locus_tag="PLT86_06000"
     CDS             complement(12412..13014)
                     /locus_tag="PLT86_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36197.1"
                     /translation="MTVACVAPAQAVSPRGAFFRSLLVPGWGQLATDAPKRAAVFAAA
                     EGALLAGAAGTAIMGNIYRDDYRALAAAVAGANLAGKGDGYFSDLAFYETRAHHNQAA
                     LIYDQPDPTLYAPEDDWQWPSTEDRMTYRRYWNRSRAMGQRLDYVLFAMSLNHLASAV
                     DAAKQASRRNPGTSAWQNVVPRVGTAAASGRVDLRCAWSF"
     gene            complement(13053..14099)
                     /locus_tag="PLT86_06005"
     CDS             complement(13053..14099)
                     /locus_tag="PLT86_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36198.1"
                     /translation="MNRQIVCLIVTLGVFFQPVSAGSMLETYHLSTRGKLASFSAIPA
                     PVASLQNESSPQMTSEEPVVTGKSPVKAFFLSLLLPGLGEWYTGHRVRSAAFLAVEGT
                     AWGLWSNFRGNGKDWENRYIAFQAAHWSFPRYDAYRHAVWDRVGQLTNFWDPSRPLSA
                     RQQDSLSVLVGAHHYDDCCGQPMPSEDDRLEMIGKYYAFSYGWDDATVYNNPNQLLRD
                     VFPAPAPGDNGVWGLNRYDWLTVLGGALVTPAYDTSSYVFQVPNLNLVQSANRDEYMS
                     MRQKSNSSYSNAEKMVTVILFNHIISAIHAARLAKSGNATTGEMPRVNTSLRMALQRT
                     DRDMVPMVMLWRRF"
     gene            complement(14204..15223)
                     /gene="ruvB"
                     /locus_tag="PLT86_06010"
     CDS             complement(14204..15223)
                     /gene="ruvB"
                     /locus_tag="PLT86_06010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT36199.1"
                     /translation="MRPITPAQLDEDRAFEATLRPTTLEEFVGQERLKERIRIFIEAA
                     RKRGDALDHVLFHGPPGLGKTTLAHVIGNELGANVRVTSGPILERPADLVGLLTKLEH
                     GDVLFIDEIHRIDRVVEEYLYSAMEDFAIDIMTGTGPGSASVRVNLEHFTMIGATTRT
                     GLLTAPMRDRFSIIERLNYYTAEELAHIVRRSARILNTPIDEGGSNEIARRSRGTPRI
                     ANRLLRRARDFAQVRGDGTVSQEIATHTLKLLDVDELGLDDMDKRILLTIMDKFGGGP
                     VGLNSIAASLNEEPDTIEEVYEPFLIQEGLLHRTPRGRVVTVSACRHFGRAGLAQGGA
                     QSALF"
BASE COUNT         3168 a         4696 c         4514 g         3094 t
ORIGIN      
        1 cgccgtcatt caccctgagc cagacctcgt ccgcggaatc cacggaactg ggtgtgagct
       61 cgatcctccc cacgagcgaa ctatcgagcc tgtcgggaat ggttatgatg aacgtcgagt
      121 cgggccgctg ggcgttcagg atgacagaca ggttccggaa cacgtatcgt ttctccacac
      181 cgccagctac gacgaactcg ctgatcagcg tgtcgcccac cccggccgag ccatcgccgt
      241 ttacatccca gttctgcccc gatccatcgt gcgcggttgg aaagactgtc agacggtatc
      301 ttccggcctt ccagaaattg agcggaatga aactggcagt cgtatcgcca agctgccaac
      361 ggcccgccac cggcggatcc acgcgtacgg cctccgccac gcttgcgggc tcgactccgc
      421 gggagtaatc aacccttatg aaatcggtgg cctgcatctc cactgaccgg tggtgcggcc
      481 atataccgcg aaaccgcgcg caacggactt ctgtccctgt cgtgaattcc agtgacgcgt
      541 ctgctcccag gggcatcccc caccggttgc acgcggcagc gcaaatggta acccgatacg
      601 tcgtgttcga atccatcggg gtatccgtac cgaggatcag cctcgtggcc tcccaccgac
      661 aatgaaacgg gatcgccggc acaacagaaa gagacgaagc cacggaggcg gagtccatga
      721 gctcatcgaa cacgaattca atcggggcgt ttctttccac gttgaccgca ccgttgtgag
      781 gctccgtttt cagaaggcct gtggtgcgcg ggggtgcttc gtaaatgcgc atatggccgc
      841 tacgcacaca gataaggatt tctgcgatac catcgttgtc cacgtcggca acgacagggt
      901 tgttcccccc ggcccccaac ggcaggctcc actggacacg cggctcgctc gcatcgttcg
      961 ttccattgta cgcgaccagg gtgttcccct gcaaaacagt gatgaattcg tcgcgcccgt
     1021 caccgtctat atctgcggca acgaaatccc atgtgccgcc ttctgttacg ctctcagtcc
     1081 attcaatcgc gccgtctgtg ccctgatagc atactaccct tccgtcaaga tggccgacga
     1141 cgatttcctt cacgccatct ccgtctccgt cccctatggc accgaagctc cggaaccctt
     1201 gtgggttctc cagggggtac caccatcgcg gcgtgccgtc gaactccatc accgtgaccg
     1261 caccggcagc atacgaaggg ctgttcatga agatctggtg ctggcctgtt ccccggaaat
     1321 ccgccacggt cggagttgcg tacgaaaccc atttgggaaa gatctcatag gcgagattct
     1381 ttatcgggta tatcgggttt cctgtctgac cgtcgtacgc cgcaaggagc gtgtacgcga
     1441 tgtggacgat gtcgtcgatt ccatcgccgt tcacgtcgtg tatggcgggt atgtaaccac
     1501 ccatcgtcag tgaggtggcc cctgtggcgg ttgttacctg tgttttccgc cagacttctg
     1561 caccggtggc tccattcaga accaggagtt ctccagaccc gcgcgccctg gcgtggagtt
     1621 gcaccacgat gtcacggatt ctgtcatcgc cgacgaaata cccactcagc cagttatcaa
     1681 ttgcggcttc cacctgtccg tcggtgatct ccgggaacgg gtgctcccat cgagttgttc
     1741 cgtcgtagtt gtacactatc agggaggcga acccctgttg agtccggttt cgggcaatga
     1801 cctccttttt gccgtctccg tcgaggtcgt ctgtcagtac actgagtggt tcgagcgtgt
     1861 aggccggcat ttcgatcatc gcgtatccgg gcctgcgcca ccgaatggcg agttcagggc
     1921 cacttgtgtc atagcacagg atgtccccgc ccgctgacgt aacgaggatc tcgtttctcc
     1981 catcgccatc gagatcgctt acgacggggg tgggccggta ccctttcggc ctgtcgttcg
     2041 ggtcctcgct cagcacattc cccgcggcat ccagtgtcac aagagcattg gtgctgaggt
     2101 ttcgcagggc gatccggcac tccgcgccgg agccatgaac gcttacgacc gcaatgggcg
     2161 cggaggagac gtactccatc cgctgcgaga ttccggccgc agcaacctgc cacgatcgca
     2221 acctttcacc cctgaaatct ccgtcggtgt cctccagtgt caggaattct tcgacaccgt
     2281 ctccatcgag atcattcagg agaaccgtct catccccatg tgcggcgata gtgttccatg
     2341 tggtatcggc accgattcgc gtgcgcacga atgtggcgtg ttccaccgtg agacgcgcct
     2401 gcgctccgga ggtagcagtc cagttgaaca cctgcaactt gccgaacaac gggggagtgg
     2461 taccaaagca ctcgatcgtg aaaagttctt tgatgccgtc gttatctgcg tcgcgaatgc
     2521 cgtagatgta gcgatcagga atattcatcg cgacggttcc gtccgcacct cggtacgcag
     2581 ttgcgtgcca cttcaggtcc tggaccgggc ggttgaaaag gctgagtgtc acttcgcacg
     2641 tgccatcgcc atccagatca ccaacggaat gaggattggg gaggactata acgtcacgtt
     2701 cgctaccgtc cacgagcgca taggtgtgct tccacaggag ggatgcgctg gaaccggcgt
     2761 ttttgattac cgacagatga gtcgcgaagt cagatatcac cacgaaatct ctgataccgt
     2821 ctccatctac atctgctgcc gcgaagtgcc cgtaattcct tccgacatcc caggtagcga
     2881 cgtaccgcgt gaaacctgtt gccgcgtcaa ggacctgaat tttgtagtgc ggagccaggc
     2941 aaatctccat cttgccatca ttgttgaagt cgcccgccac gacttccggg ctgtacatgt
     3001 cgcccagatt cggcgtactc cacgcaagac ggccgttcga gatcccgccg tggaaactga
     3061 acatgtatcc ccgcccctca ccggtgccca tgtccgcggt gaaggcaacc acctcaagcc
     3121 cggagctatc aggaaggaaa tcggcaacct gtgacagtgc cgacgagaat gtcttcccgg
     3181 tcgtgttgtc ttgccacaac agcgcgccat cgtcgattcg gcggatcctc agggtcccga
     3241 acagcaattg ctctcgccgt ccgtcgccgt cgaaatcaaa cacccacgaa ctgtcggttt
     3301 cgtcagatcc tgcaagatcg ggaaccgggc cgcccagata ggtttctgaa cgaaggaccg
     3361 gcgcctctcg catacgactg accgcgtctg tacgcccggt ctgatgcggg ttctggcgat
     3421 acatcggcca ctctccgcct gcggctcgcg tttcgtccgc agcaccacac agcatgcaga
     3481 atacagcagc aagcatgaac acgttcattt tcccacctcc cgcaaccgta tcgtttcacg
     3541 gttgtgttgc tgatcgcact gcaacagatc gccacagaat acctaacgaa aaatgatcgg
     3601 gcgcccaaac ggtagtccat tccgaccgtg gttcgccgcg aggtcactgc gtattttttg
     3661 tgatcgtcaa cgtcaaatgc cacggcacgg gaccagccat gcaggccaga actcgataca
     3721 acgctccgct tacggactcc gccgacgtcc gcgattcgat ctgcggtcag ggcgcgggcg
     3781 cgtacctgct gcttggcgac aacggagtag atatcgagga gacggtagcc ggcgttgtcg
     3841 cccatttcac tgacgaagca acccgggcgt tcaattgcga cgtttttcac gtcggagagg
     3901 ctgaatgcac ggcagaagcc gttgtcaatt ccgtgcgctc ctatcccgtg cttgccgagg
     3961 tccgggtcgt cattgtccgt ggtatcgaag gcgcaaacgc cgcatttggt aacgagctcg
     4021 tgctgctcgt tgagaacctc ccgccggcag ttgttctggt catgagcggc gaaaagcttg
     4081 atgagcggac gaagtgggcc tcagttctga agaaacgttg caccgtcttc acattcgccg
     4141 ttccgcgaaa cgtgaccccg tggatccgac agcgcgcgaa atcgcgcggg ttcacgtttg
     4201 atgacgatgc agcggagttg ttgagtgact ttgtcggaac caacgtgttc cgcgccgccg
     4261 acgaaatcga aaagctcacg ctgttcgtgc agccgaggaa gactatcagt gcggaggatg
     4321 ttgaggcagt agccggaatc gcacgcaccg acaccgtgta ccagttgaac gatgcgatcg
     4381 acggtggcca ctctgaacgg gcccttcaga ttgcacatcg gttgcaggag cactcgatgc
     4441 aggcggccac aattgccggg ttgatcctgc agcactggat gcagcttcgg gcggctgctg
     4501 acgtccagca gaacccggat gagatcccgt tgaaggaact tatcggggag aatcgggatt
     4561 ttgtactcaa gaagctgacg gactccgccc gtgcgctggg acgggaccgc attcgacggg
     4621 gagtgaggct tgctctggcc gccgaaagcg cgatgaaaag ctcgtggctc gccgacaaat
     4681 cggtgggact cgacgcgctg attctccagc tttcagtgcc accagggggc cgtgctcctc
     4741 gtggccattc agcgtacgcg cgaggcaaga cttgactgac cgaaataccc ggtcgtactc
     4801 ttaattactc catgcagacg caccgcggtg tcgtttgatg acggcgtggc ggcgcgggaa
     4861 aggatctgga agatggcgaa acctgtcgtc gtaacggacg ccacattcga ccaggaggtg
     4921 ttgaaaagcg acatcctcac cgtggtggat ttctgggcaa catggtgtgg accgtgccgg
     4981 atgatcgcac ctgtggtgga agaactcgcc gcagcgtacg acggcaaggt gaagttcgcc
     5041 aaggtcgacg tggacgaaaa ccagcagcat gcgggccagt acggcatccg cagcattccg
     5101 tcccttctgt ttttcaaggg cggaaaagtc gtagatcagg ttgtgggcgc cgtgccaaaa
     5161 ggaacgctgg aaacgaagat caaagcgcag ttggggtaaa tcaggatcag gccaacgtag
     5221 ctcagcaggt agagcagctc actcgtaatg agcaggtccg cggttcgatt ccgcgcgttg
     5281 gctccataga gaaagaagcc cgaatcggaa ctaccctttt cgggcttctt cgtcttttct
     5341 ctgcagaggg ggcgtggcat gaatgtgaga gatttccggc aaccggaaca cctcgtcagc
     5401 gagccgtttc tccagaggtg gtctccgcgt gcgctgacgg gagagcccat caccgaagat
     5461 gagctcaacg ggcttttcga ggcggccaag tgggcacctt cggctttcaa taatcagcca
     5521 tggcgttttt tgtacgcgca cagaggcagc cccgagtggg acgtattctc cgacctcctt
     5581 gtggagggca acaaggcgtg ggcgaaaaac gccggcgcgc tgattctgct cgtctcaaaa
     5641 aggaccctgg accacaacgg ccagccttcc cgcactcact cgttcgacac gggagcggcg
     5701 tggatgagtt tcgcacttga agcctctctg cgcgggctcg cagcacacgc catggccggt
     5761 ttcaacgcga acaaagccca gcaggttctc ggagttccag aggatttcgc gattgaggtc
     5821 atgattgcgg tcggacggca gggaccaccg gagatattgc caccggatct tcgcgaaaga
     5881 gagcggccga acacgaggaa gtcggtgtcc gaaatcgcca gggggggagt gtggaaggac
     5941 tggcggtgat ggctctacag ggtcaccttt gcgcgacctg tgcgtagaaa acgccttcca
     6001 gacgttttgc cagtacctcc atcagcgtgg cgtaacgtac gcccgctgcg taccacgtgg
     6061 tgggatcgtc tggaactacc gggggttcca cgtcaaccct cgcgaggaac gccatcgtgc
     6121 gggaaagatc cggatcagcg accggagcga gtatcaatgt gcttttcccc gcgatataac
     6181 acagcgtccg ccacgtgatt ctgtcgtcgt ggggaaacac gtacagtgag tcctgcgaga
     6241 gaacagtgcg ggccgcgagg agcgcctcgc catgctggcc ctgcgcgtcg aagattatgg
     6301 cgcctgccgc cgttgcgtga atgtacgcgg ggtcctgaga cgagatccgt ccaatcgcag
     6361 tgagggccga cgccagtcta tcctggcgga tgagggacca cgccaccccg agttgcgctt
     6421 caccgtcccg ggaatccagt tccgccgcct ggcggaattt cgtctccgcc tctgtaaagt
     6481 ttcccgcccg ataggctgtc catccctctt ctcgataccg ctcccgttgt tgatcgggag
     6541 acagcggcct ggagcacccg accagagcca gcatcaggac acacatcgct gagacgtgcg
     6601 gtttcatcga accaccacca tgcggcgcac gcgcgtttcc gcggggagac cgtcccggat
     6661 tgccgtcagt cgagcgagga agatcccgct ggccacgcct ttgcccgtct catcaagtcc
     6721 gtcccattcc acggaggacc tcccgggagc aacggcaaag gatcgcacga gttgtccctc
     6781 aacggtgaac acctccagcc gacccccttc gtaccgtgcc gggatactca cgggaatgcg
     6841 ggtggatgga ttgaacgggt tcggcgcatt ctggcccagt gagaactcga ccgggcgccc
     6901 cgccgcgttt gtgattcggt acgttccggg ttcggcgagg acaacccagt atcgcccacg
     6961 tgtcgggtca aaggaaaatg gctccgtgtg gccggtggtg actgactgga gcgcgtttcc
     7021 ccgtgagccc cacgcgcgcg gcaatgccac gcgcaccggg atcgtccacc gggccccgtc
     7081 cggcccaagc cagattgagc cgtcggcgaa cacccttgcc agaacgatca tgccgccccc
     7141 ggttcccggt gcgagggtga actccacggg ctctcctccg gaaccggaga ccacgcgcgc
     7201 cacacctcct cccggcgtaa gggcggcata ggtggcagag ggctgtgccg gtgaattgcc
     7261 cgccagatca acggcgcgaa gcacgaaatc gaacgcgccc gttgacgtga gcgcaacgtt
     7321 gccaacccag cacatgcccg tggaatctcg cggaacaagc tcgacaaccg tggtgccgtt
     7381 caacaggaga ctcactggaa ttgtcccatt tcggtacaat ggttcgtcgg aaagtaccag
     7441 ggcggtgacg tacgcgggca acacggggtt tcccagcagg ttaatgacga cgttcggggc
     7501 ggcgaggtcc gcctcccccg taccgaaact ccaggaaagt tcctctgagg acagtacgcc
     7561 gccgaaaggg ctgtggagat cgcttaccgt cacccgaatc acatcatcgg cgggtaacgg
     7621 cgcatcgctt gagtcaatca tctcgagcgt ttcgccgcac gtgcctttca ggccgcttct
     7681 caagacgcgc aaatcgcggt cgaggacacc tgtgcgggcg ctcgacacga tgactcgtac
     7741 atcctgtccg gggtccacgt tcgaaacgat caatttgatc cgtgcgcggt agatgtcggt
     7801 cccggttccc agaggttcgc gctgctccgc taccgggttc agcgttccgg gcgcctgcca
     7861 cgagagatcc atcacatggt tcacggtaac catgcctgtg ggagcgatca caagccaggg
     7921 ctgccggagg gagcaatccc ggaacacttt tgtgccggac ggaaaccacg atcggataac
     7981 cggctcgttg gttaccgcct cagagttctc caccgaccgg atgttccaca cgcctccgga
     8041 acttacggtg atagtcccct gggctgtctg ttcggcagcg cgcagataac ggacgccata
     8101 actggcaagg gtatcgcgga gggagcacgg gaacgctgtt cttccaaagt cccccggaaa
     8161 cgccgcgcgg gcgtcgaacc atgcgtagcg gccgggtgtg tttcgcgccg ggggtaacaa
     8221 attcgctacc gtccagtcgg gaaacacatc gcgctcaaac cgcgaggaaa aaccgcggga
     8281 agccagaacc gcgtcgattc ctgagatccc atgctggggc gagcgaacga tttcgccaat
     8341 tgtctgcact ccgaactgat ctgcgaggta ttgcatgatc atgtaagact tatcataatc
     8401 acccaggctt ggagtcgcgg ttccatcgtt gaacaggccg atatctgtat tcgcaaagaa
     8461 ctcgcgccct gtgtttccct ggtacccgca gagaacctgc gccagcacgg cgattccctc
     8521 gtcgatccac ctctcctcgt accgatcgat gcccatgtga atcacgtgga ggaattcgtg
     8581 agcgagcgtg cgttcccgct gaacggcggt cgtgcgcgac aacccgtcca aaacgacaag
     8641 gttcctgaag ttggaacggt cgtacaacga gggatcaaca cggtcgttcg ggcttacgta
     8701 gccaacgatg tagccgccgg tgtaattcga tttgatttcg gtgagagcga tgtacaccag
     8761 tgaatccccg ccaacgggat tgaccggagc gaacgcggta gtaagcacgt cgtagatacc
     8821 cttgcccacc ggcgccggcg atcccgcgtt ctcctcgaac gctgcactca ggcgtgtcac
     8881 atccgccgga tcaatcgctg tggcgctggg caaggcagac cagcacgcgt ctgcaacgag
     8941 gatgacgctc cgccggttca ctgcacgaca cgtgaccgac acctcctcaa gctgccccgc
     9001 cgcgaaattc tgaatccaga gggtttcctt tgcccccacg acgggattgc gagcgggttt
     9061 tgcgcgggac acgtcttcgc ggaagaacga ttcgggcagc gccgtgaaga ggtgcgagcc
     9121 acacggtatg ggcgcggcag catcagcgga ggcaaatccg gctgcgcaca ggacggccac
     9181 gtatgcgctg gttctgactc tcatggtttg tgttcttgtt accgcgagtc gtgatgccaa
     9241 cgggtggatt cacacatgtg gtaccattac atccacgggt gcgcgggagt ttcatcccgt
     9301 tcagaaatcc gccccgaggg accagtactg acgcgacccg ccaagcgtcc gatcgaggtc
     9361 cgtcggccac gcaatatccc agcgcagaac tgcaacgccg agattcatcc gccagccgaa
     9421 cccgaatcca ccaactgcca gtggaccgcg tgtcggggcg ttcgtcatgc cggcagatgt
     9481 gtaattcagc ttgattccgt ttcccgcgaa gggagcaaaa cctctcccgt cgccccacac
     9541 ggttcccagg tcgagaaaaa ggattcccct gatgttccgc agtacgatcg gcaggggcca
     9601 tccccaggcg agctcacgaa caagggggta cctgaattcg atgttcgcaa gggcgtatct
     9661 gcttcccgaa agctcaccga agcgactccc gcgaagaggc gtaatgaact gggtgatcgg
     9721 tatgaggctg tcgttcgtaa cgctgtacgt cggagtgtag tggaagttga tccagtagct
     9781 cgtgcccccc agcaggaacc ttcccgggtt ccgaccgaca ctcattccgc tggatacacg
     9841 taacgccagc gcgtagtccg cggatactcg aaggtacttg cggtaatcgc caatcagcgt
     9901 cgtatacgcg taggtgttga aaccgatttc gggcgcgaac ttgacctgaa acgctgatcg
     9961 ggagccgttt gtcgggccgg tcacccccca aagcgtattg tcgttgataa gcgcgaacga
    10021 gggaagaagc gttcggacac cgggaagacg ccggaccggc gtgcggagat aggtatcata
    10081 cgagaagttc gcgcgctcgc ggtcagaaag ctccagcgac gtttcgaggc gggtgaactt
    10141 gttgaaaggg tagttcagaa gaaccgcccc gccgagtacg cggtcgctcg tccattccgt
    10201 tgccgcaaag ctttggagat agtactggtg aaactggaaa agcgaaaatc cccagtttgg
    10261 tctcttccgg aggttgtagt acgcaaactg gaagtccgcg tcttcgacat tccggtagat
    10321 atgggtgtag atctggatct gatggtcgcc catcgtgtct gatacgcgaa gcatcgtctg
    10381 cgccatcagg ccgttgatgt tatcgaacat cacaagtccg gagacgaggt ccggcgagaa
    10441 gtgcacatcg taaggctttg acacgaaggt gagaggatta ccggaggaat cgtgcggttg
    10501 cagcgagaac ctccagtgcc ccagaccgga aacaggggga cgctccagat aacgggagga
    10561 atcgctctgc gcttcttctc tgcggcgttc cagaaactcg gcgggcggaa gcgggtcatc
    10621 cgcctgccat ttagccagag gatcggtcaa cgtgaatatg tcgtatcccg ctacgttgaa
    10681 tgcggcaacc gcaatcttcc tcccgtccct tgaccagtca ggctgaaaga caccgtcgat
    10741 cacattcgtc agtggtcgct cggtgccgct gtcgaggtca gcgacgtaca ggttgtaaat
    10801 tccgttgcga tcactcgtga aggccaatct gcggccatca ggtgaccaac acggagatga
    10861 atcctccccc tccgcccgcg tgatctgtgt gatcacgccg gtagacacgt ccatgacaaa
    10921 cacgttgcga tcaaatccca cgggcaggac gttggagtct tcaccgtcca gtacccggtc
    10981 gctggcgaat gcgatgcgcg tgccatcagg cgaccacctg ggtgcaatct cgtcgtagaa
    11041 gtcatctgta agctgcctca gttcacccgt gagcgtgttc accaggtaca ggtcgctgcg
    11101 cccttctctc acaccgacga acacgatatt caggctgtcc ggagaccagg aaggcgaaaa
    11161 cagcgcgttg aactgccgcc accggaacat gcgctgtatc cggccatttt ccgcggaaac
    11221 gatatacaac gcgtcgagat cgtgatgctt tgccgcgaac acgagatgag acccgccggg
    11281 tgaccaactg aggctcccgc gtgaccagtg catctcttcg aaatggctcg aacgttcccc
    11341 cgtgaccacc ttccggagga tgcgcccgtc tatcgccgac atgatgtaaa tcgaggcgta
    11401 gtcatctcga tcggaaagga acgcgatcct gtcgccgtgc ggggagaacg ccggcgcgaa
    11461 gttgtaatga ttccggtctt tacggtggtc agtgaggcgt cttcccatct catcggtcag
    11521 ttcccgccgt tcgatttccg gccagtagaa acgtttcaga tgccgttccc agcgcgtttg
    11581 gaggtcgtca aggctcacac cgaggaccgc ccggaacgac gccgcaggtt ctttcgtgat
    11641 tgccatccgg cgggttattg cggggatttt gtccgcacca aactcgtccg cgatccagcg
    11701 aaggagtgag taacccgcgg tgtatgcctg gtacccgtcg agccgcaggt atccatcgat
    11761 cgttgcccct cgaacccaca tgttcttttc ggtcgtccaa tccgtcgaga ggtactccgc
    11821 cagcccttcc gcgaaccaga gaggaggacc ccaccagagc tgggaagcag tgctcaaccc
    11881 gccctgacgc cggtagaaca tgtcaaacat cacggcgtgg acaagctcgt ggtggatcac
    11941 gtggcggaac tgggtatagg agcccgtgaa cggaacgact acgcggttct tgaatgcctc
    12001 agtgaaaccg agcgtgcttt cgtcgatcaa tccccacgtg gcgttggtct gttcaaagtc
    12061 gttgtgagag tcgtagacga ggagaggaac gggttgggac aggcggtaat cgagcgtgcg
    12121 ggagattcgg gcgtacgcgt cttccgccat ttgcgacgcc acgcgtgcga tgtcaccctc
    12181 accttgatag taataaaccc ggaagttctc tgtttcgatt actctccatt caaacgcctg
    12241 gtaattgacc ttattgcgtc cgaaatggga tatctcgttc caccactggg cagaagccgg
    12301 cgttgcgagg aggccaaccc cccagatcat ggcgatccac ccggcaatca cccgaccacc
    12361 agcgcccctg ccgacgccca ttaacgtcca cctcacgggc gttctgctcg ttcagaagct
    12421 ccatgcacac cgaaggtcca cccggcccga cgcagctgcc gtgccaacgc gcggaacaac
    12481 gttctgccac gcggaagtgc caggattccg tctcgaggcc tgctttgcgg cgtcgaccgc
    12541 agacgcaagg tggttcagtg acattgcaaa caacacgtaa tcaaggcgtt ggcccatcgc
    12601 cctggagcga ttccagtacc ggcggtaggt catccggtcc tctgtggatg gccattgcca
    12661 gtcatcctcc ggcgcataca gtgtcgggtc tggctggtcg tagatgagcg ctgcctgatt
    12721 gtggtgtgca cgggtttcgt aaaacgccag atcgctgaag tatccgtcgc ctttcccggc
    12781 aagattcgcc ccggctacgg ccgctgccag cgcccggtaa tcgtcacgat agatgttccc
    12841 cattattgcg gttcccgcgg cgcctgcaag cagggcacct tccgcggccg caaacacggc
    12901 ggcacgtttc ggcgcgtctg ttgccagttg gccccacccc ggcaccagga gggaacgaaa
    12961 gaaggcacct ctggggctga ccgcttgcgc aggggccaca cacgcgaccg tcatgaatgc
    13021 acaccatacc cacaaaggag agcgtgtgcg cattagaatc gtctccacag catcaccatc
    13081 ggcaccatgt ctcgatcagt ccgctgaagc gccatccgca ggctggtgtt cacgcgcggc
    13141 atttctccgg ttgtcgcgtt cccggatttg gccagccgcg cggcatgaat tgcgctgata
    13201 atgtggttga acagaatgac tgtcaccatt ttctccgcat tgctgtaact gctgttggat
    13261 ttctggcgca tgctcatgta ttcgtcacgg ttcgccgact gaacaaggtt caagtttggc
    13321 acctggaaca catacgacga ggtatcgtac gccggagtta cgagagcgcc gccaagaact
    13381 gtcaaccaat cgtagcggtt gagcccccac accccgttat cgcccggcgc gggagccgga
    13441 aacacatccc gaaggagctg attcgggttg ttgtataccg tcgcgtcatc ccagccatac
    13501 gaaaacgcgt agtatttccc gatcatctcc agcctgtcgt cctcagacgg catgggctgc
    13561 ccgcagcaat cgtcatagtg gtgcgcaccc acaaggaccg aaaggctatc ctgctgccgg
    13621 gctgacaggg gacgggaagg gtcccagaaa ttggtgagct ggccaacgcg gtcccacacg
    13681 gcgtgtcggt acgcgtcgta cctcgggaag ctccagtgag cggcctggaa ggcgatgtag
    13741 cggttttccc agtccttccc gttgccccgg aaattcgacc agagacccca cgccgttcct
    13801 tccaccgcca gaaatgcggc gctccgtacc ctgtggccgg tgtaccattc gccgagaccg
    13861 ggcaggagca gcgacaggaa aaatgccttc acgggtgact tgcccgtgac cacaggttct
    13921 tcggacgtca tctgaggaga tgactcattc tgaagcgacg cgaccggtgc cggaatggcg
    13981 gaaaaagaag ccagcttccc ccgcgtgctc agatgatagg tctcgagcat cgacccggcg
    14041 gaaaccggct ggaaaaacac gccgagagtg actatcagac atacaatctg ccgattcatt
    14101 atcgcgagct ccttgccaga ctgtccgcca ccggatttgg atgcaacaag ccgttcgtcg
    14161 ttatctcgta gagtgtctcg accgcgaaag agttcccccg aattcagaat aatgcgctct
    14221 gcgcacctcc ctgggcgagc cccgcacgac caaaatggcg gcatgcggaa accgtcacca
    14281 cacggcccct cggagtgcgg tggagaaggc cttcctggat gaggaacggt tcatacacct
    14341 cttcaatggt gtccggttcc tcattcagag aggctgcgat gctgttgagt ccgacaggac
    14401 cgcccccgaa tttgtccata atggtaagca ggatgcgttt gtccatgtcg tcaagcccga
    14461 gttcgtcaac atcgagcaac ttcagggtgt gcgtcgctat ttcctgactc accgttccgt
    14521 caccccgtac ctgagcgaaa tctcgcgccc gccgaagcag acggttcgca atgcggggtg
    14581 tcccgcgcga gcggcgagca atctcgttgc tccctccttc gtcgatcgga gtgttgagaa
    14641 tgcgtgccga ccgccgaaca atgtgagcca gttcctcggc agtgtagtag ttcaggcgtt
    14701 cgataatgct gaagcgatcg cgcatgggag cagtgagcaa acccgtccgc gtagttgcgc
    14761 cgatcatcgt gaagtgctcg aggttgactc gcacggaggc agacccgggg ccggtccccg
    14821 tcatgatatc gatcgcgaaa tcctccatcg ccgaatagag atattcttcc acaacacggt
    14881 ctatgcgatg gatttcgtcg atgaacagaa catccccgtg ttccagcttc gtaagcaatc
    14941 ctacaagatc ggcggggcgt tccagaatgg gacctgaggt gacacgaaca ttggcgccca
    15001 gttcgttccc tatcacgtgt gccagcgtcg ttttccccag acctggcggc ccgtggaaaa
    15061 gaacgtgatc gagagcgtct ccccttttcc gcgcggcttc gatgaagatg cggatccgtt
    15121 ctttgagacg ctcctggccc acgaattctt ccagcgtggt tggacgaagc gtcgcctcga
    15181 acgcgcgatc ctcatcaagt tgcgccggcg ttattgggcg cacggcatcg cgctcgttct
    15241 gcaccaatca acctccagaa ctctagaaaa accctgcccg gttgggcgcc atagccggaa
    15301 acatccgctt acagggagcg caacgcctct ttcacaaggc tttccggtgt ggcgttcgcg
    15361 ttctgtcgct cggcggcgga ccgcaccgcc tgagccgcgt cggcatgtcg gaagccaaga
    15421 ttcaccagcg caagaacagc ttccgtctgc gcatccgcag cgtctttccc cg
//
LOCUS       DAOSOO010000059        15240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3920_length_15240_cov_4.593876, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000059 DAOSOO010000000
VERSION     DAOSOO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15240
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_15240_cov_4.593876"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="PLT86_06015"
     CDS             complement(<1..1926)
                     /locus_tag="PLT86_06015"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOT36200.1"
                     /translation="MGLDDIFPTVPPVRRLLATAPDLENEGLDFWIWFRSLQGLVNRA
                     EPHLYLIKGGEVHKQGREYWERHWLDYYTNPDTSPFKIPLQRENSVDAIVERFRDRVN
                     GYILYDMADVPQTMNLAITLAGLEGCLPVAVDQESWMLRHGIPRRDDLRGRFSDDWQA
                     AEWAVDNLWPQCNQRLIANFCIHRPGWSSIPHELEDYVVANRIFAIDLPASRQRRRVL
                     RLLHRLLESAHAPGALLNWHCVFDQEKEYVAEAAKRGFFVLCSMSSPNLTIHGGVGDT
                     GRSYHQPLPERASCVAEPDKTYVCLYNSDGDATWATNSMQSGNWTEPNRGKFKFGWGL
                     LPLALKLQPGMVQLFHETKTPNDCFWGPSSGAGYTYSHLWPAHLVDLYLSESRRLLAQ
                     SGQNGCNMVNWYLQDWWREVEDDAAVRREQEALKEGPGLVCGLGGSPYAKSYLDGPIP
                     KLHSVHIANVGHNTVADIIKFREECPTRPQFMFLFAQISPGIWKQIESELDEYAAHPE
                     IVLLTMDEFFLTLQDAIKKGLVKDSLYEITEPMAERWLKAPGRHRLPICEQVTRELAD
                     TAHAEPTERRLRLAETAWTELVSREVENVARDRERFTTHFKGRTPFTEAEEADALHYT
                     AFTVAWVVVRAAIQAQGV"
     gene            complement(1942..4098)
                     /locus_tag="PLT86_06020"
     CDS             complement(1942..4098)
                     /locus_tag="PLT86_06020"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOT36201.1"
                     /translation="MQLTDVFPRIPPVKRLSVARINPQGEGIDFWIWFRSLQGLANRM
                     EPHLYLIDLNAMEKHKPLGAAETHWLDYYRTRFGIPTEDVESVDAVVERYKNLIDGYV
                     LYDNEQIIQTQNLAITRVGLECLLPVSPDQEHWMQRHGIVKRDDLRGKFADDWDAAEW
                     AIEHLWPSCYRKIYGNFCIHRPNWYALGHQLEDFIVMHRGMALDLPRCRTHRRSLYLY
                     RKMMESADPPGCQMNWHCAWEQEKEYVAEAARKGFFVLCSTGTPNLSIHGGIGDPNRS
                     YVQPLPARESCKAENGKVYVCFYNSDGDATWAMNNLHSGNWLVPERGKFKFGWGFLPL
                     MVKLMPGMLQYYQETKTEADCFWGPSSGAAYTYTHKWPTNLVDMYLTESRKLLDQTGQ
                     NGCNLVNWFLQDWWREVEDEKAVRREQEAMASGPGLVCGLGGSPFAKSYLAGPIPKVH
                     SVHIANVGRDNVGDIVRFSRECPTRPLFMFLFAQISVGIWEQIESELEEYKKHPEIEI
                     LSMDQFFLTLQDAVKRGLVGDELYEKTEALAETWLKAPGRHRLPLCERLAEELAHVAR
                     MPSDERRRHIGDAAWTDLVSIEIEGVARDREKFLTRFKGRKPPDATEDSDALLYVAFT
                     TAWVVVRAAIEAQGVYANHRDQCLNDFRRLCAAICDVTPFERLFDAWDKWETGTPSVA
                     ELAPLCEGVAAAARKLTDALGASGEEEFTGWPPRTI"
     gene            complement(4153..5331)
                     /locus_tag="PLT86_06025"
     CDS             complement(4153..5331)
                     /locus_tag="PLT86_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36202.1"
                     /translation="MEHVVPPSRILPEASQERSKVLTTITVGLLGAGRGLSLAQLFDR
                     VPGYRVVAVCDAIPERLDRARSACPRAEGFRDYAAMLREPFDVMIVASAPPSHADHVC
                     AALEAGKAVLSEVPAAHTLDDAQRIVDTVARTSGYYMLGENCNYYGFIHAWKKLLEEG
                     VLGEVIHTEGEYVHDIRGITWVDADGRYYNPADPRCPATARPSWRARYNPIQYITHSL
                     GPLLWLTSDRCTSVSCLSTGCRTQPEIGSPDGQIALFETNNGAPIRQSCIFSAPKEPG
                     GQWYSLYCSRGYAESKRAPWDSGKLYLADGTMQEIQRKEWPAAVETPLASSGHGGIDG
                     GLVWDFAHAFRRGAPSPIDVHTAMDYTLPGIYAALSAERNGERVRIPDTRTERLVTRS
                     "
     gene            complement(5353..6228)
                     /locus_tag="PLT86_06030"
     CDS             complement(5353..6228)
                     /locus_tag="PLT86_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36203.1"
                     /translation="MDNIFRNKMVQGIGLGIAICIVVVGAFFGIKSLMGPGSLKEQLT
                     GQKKYQPPSSASTDSAFAFPTIFMRVVGDSVRGLVSIDPTKKIDAGTELGVQEMVNRL
                     RVGHQTLAQNPGPRGVPNAFNLVLDKSSTYGQAMLGIYIGVNGTFQMPFLITAEGNQH
                     SVEPPRMHPQFGPMGWAAWIWWLPDQRIKWVVWPDSLEGNFTFHVLPAKNGKPDIEGL
                     RGKLRTAMDGKAHIPPPQQRLIGFWGYDSLPYESAIQFFDVLRYPKTGEPLCSRLTLM
                     EGEMMSPPADSVCVE"
     gene            complement(6396..8801)
                     /locus_tag="PLT86_06035"
     CDS             complement(6396..8801)
                     /locus_tag="PLT86_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT36204.1"
                     /translation="MEVEAGQSWRISGWFRVKKGLVFLRTEFLNSAGAVLGSADTPEI
                     AKDRHASGWRYTAVETDAPASAEHVRMSLIVRGEALCDDIVMTPLIANLLYNPTFEAG
                     NRGRVTFWDPDPVLTRATAKTGSGIADETGGRNGAGLRIRSDSSWWAMRNIQQPIPVG
                     LTTFRLSGWVRWEGAAPDIAIAWTDNGGVLHGLSPATPCETLNGWTRFQVSDAHPPVQ
                     AETMTVSLVGNIGTAWFDDFQLEPVAPATNLRRVVKVHVNQVGYERNLPKSCVVATNF
                     VPHGGRNFIEVHGEDGHTLGSFPLVGPERIHNGMPDDWGEYFWRADFSSLKETGRCTI
                     TAVVGGVRGESYPFLIGDGILFGGTAPLGVEFFFVQRCGFAVPGWHAACHLDDALLPD
                     GTHIDAVGGWHSAGDYNKLMYENGDGGVLYALTEAFRTKEQVFENSDRDNDGVPDILD
                     EARWGADFVVKMQVPETGGLRKDVSQGPGRMWMKWCPPEVHTDNIIGTDDDPVILPGE
                     GNSPLAIGGWMRLAGELERRGEDGSKYGSSAERLWGYSTQNGETGFSPLNLLSALDLH
                     AATGERRYAEFASRCVDTLLASQVPSGRHAGAFGAFGEHAAGALGTYALKFPEDFRIP
                     RIRAALARYIEFCLTTAENPFGLAKQSVGEQDFFFEPTSTLGHNFEILARAWSAIIAY
                     QATGQEAALTFALDQIDWVMGKNPYGLCMMEGAGSNNPPRYHHRYDSIPGRERGAVPG
                     AIPNGFVRTPRALDQPGFDMSRSRTERDHPSYRTSEPWLVHNMWHLLAVSALQRVVDE
                     RAR"
     gene            9198..10259
                     /locus_tag="PLT86_06040"
     CDS             9198..10259
                     /locus_tag="PLT86_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36205.1"
                     /translation="MRERILAVIRGNPHDRVPFVQYDGLAAPNEVVWDVVGRANMGII
                     RWSSAYRIEAPDCVITHEPVEINGRTGERARLITPRGSLVQERVFEPAYQSGATRKHY
                     VCDSADYEILLSYLRNVVVVDDATPFLLDRDALGDDGVPMVAVHRTPYQQMWIQWVSL
                     EQLAVDLEDDLPVLRECLEVLGNIERRIFESVCRATREHGVPFVDFPDNITAPAIGER
                     YFRGYCVPFYDELAGMLRDDDAPVFVHMDGDLKPLWEAIGGSGVRGIDSLSPPPDNDT
                     SCADAATRWPDMRLFVNFPSSVHLWEPEQIYSHAMAMLRQAGHTGRMQIQISENVPPG
                     VWQKSFPVIVEAVRDFGKP"
     gene            10279..11307
                     /locus_tag="PLT86_06045"
     CDS             10279..11307
                     /locus_tag="PLT86_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT36206.1"
                     /translation="MDTYSFAPELCHEYLMDRAPLALTFDPHKHSDAESFDAWRRKVG
                     AKLRQLLGNTPAKVPLNIRHEFETQHEEFVERRFVFTAEKNADVPCHLLLPTHGSAPF
                     PVMICLQGHSTGMHISLARPKYEGDQKSISGGDRDFAIRAVKEGFAALVMEQRCFGER
                     KDAKHEGSRCHHATMVSLLLGRTMIGERVWDVSRAIDALATFPELDSDKIACMGNSGG
                     GTITYFAACCDPRIKAAMPSCYVCTFRDSIGRIHHCEDNYVPGILKWFEMGDLACLIA
                     PRPLVVVAGKEDEIFPIKGVRSTFRRIRDIYSAAGHKDKCRLVVGNGGHRFYADDAWP
                     RFKQLTRW"
     gene            11357..12781
                     /gene="melA"
                     /locus_tag="PLT86_06050"
     CDS             11357..12781
                     /gene="melA"
                     /locus_tag="PLT86_06050"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT36207.1"
                     /translation="MTKVAMMGAGSAGFCRMLVEDILSFESMKDIHFALMDVDPERLD
                     LVYRSMLRMKEEHTLACTFSATTDRREALTGADFAISMIQVGGLEPYKLDVSIPLKYG
                     IDVCVGDTVNPGGIFRGLRHVPALLDMIRDMQDVSHPEIIFMNYANPMAIVSWAMQKM
                     YPDTTSVGLCHGVQHTTNMLCQWLGVAVEECDVLTAGINHMAWFLKFRHNGSDLYPRM
                     WDKLAAEGPIRGEEYRFEMMKATGYFMTESSGHLSEYLPYFRKRKDLLELFNGPGFSG
                     ETAYYLNSCLQHRDKYLEEMRELADGRKPVPFRRGYKSVEYASNIMNAKLTGEITRFA
                     GNVLNKGFITNLPYDCCVEVPVFVDRLGLHASYVGHIPEQCAALCQSNISAQELAVKA
                     AINGDPEAVYHACMLDPLTAAVLAPHEIRNMVDEMLEAQAPWLPQFKGKSSHAPGHRV
                     GRLQTGAKSLVVGAEVSSKKGHYD"
     gene            12857..13912
                     /locus_tag="PLT86_06055"
     CDS             12857..13912
                     /locus_tag="PLT86_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36208.1"
                     /translation="MSRKIRVAVTGAGGEARWYMEAYKTCPDSELVLVQDIDPAKARE
                     QGERLNVNWTTTFEDLLGPDIDLVDVSTPNHLHAQQTIAAHEAGKHVICQKPMAPTIR
                     DCDEMIAAAQRNNRKLGLYMSSLGAPLFHEIKEMIRQGFFGIISSVSSRGAHRGGYEA
                     KDNTAWRGSAEKTGGGVFIQLAVHSINLFQWLLDDDIVQVAALSTNRLCKHSIGGDDL
                     THAVAEFRGGVYGSFEAGYAAEGGLFALYGTKGSLVMTAGQTFLWSELPWDGDIIKYT
                     PRGGKEMTVLDPADYGPRQARFAGKYDQHAAFVRAILEDAPVPVPGEIGRRDVAIVKA
                     VYRSAEERRHVLIEEMG"
     gene            13963..14286
                     /locus_tag="PLT86_06060"
     CDS             13963..14286
                     /locus_tag="PLT86_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36209.1"
                     /translation="MRHVSLYDLPDGNDQAELLANLPASYGSFGASVRFFARGEATEA
                     QRASNGVLLLVAQGSCVVRVDETEYPAARFDLFALESGETFEICADEIDPAVVLRIDA
                     GNSEG"
     gene            14295..15023
                     /locus_tag="PLT86_06065"
     CDS             14295..15023
                     /locus_tag="PLT86_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT36210.1"
                     /translation="MFFGSQDFFILLLIAFGAAVVQGTLGFAFGMVAMALAALVIDVR
                     TASILVSPLAFVNVSTTLWSVRRSIRWKLVLPVVGGTLVGIPFGLAILLGGNLILIRF
                     LVSALLVYVGVTSLIGFKVRRHALPGWVAAVAGLGVGVIGGATNIGGPPLVAYASRQP
                     WDAETFKATLLTTFFVSSGTKSALLIANGAIDARMLACIAGLVPAIIAGSLIGVHLFN
                     RINKERFKRIISWTLLVLAVLVQV"
     gene            15057..>15240
                     /locus_tag="PLT86_06070"
     CDS             15057..>15240
                     /locus_tag="PLT86_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT36211.1"
                     /translation="MDIDGTTRVVGVIGDPVSHSLSPAMHNAAFEALGLNWVYLAWHV
                     APEGVEQAVNAVRGLDL"
BASE COUNT         3059 a         4492 c         4435 g         3254 t
ORIGIN      
        1 gacgccttga gcctgaatcg ctgccctgac aaccacccat gccacggtga acgccgtgta
       61 gtggagcgca tccgcttcct cagcttccgt gaacggagtt cgccccttga aatgcgtggt
      121 aaaccgttcg cgatcacggg cgacgttttc cacttcgcgc gagacgagtt ccgtccacgc
      181 tgtctccgcg aggcgcaggc gacgctcggt tggttccgcg tgggcggtgt cggccagctc
      241 ccgcgtcacc tgttcgcaga tcggcagtct gtgccggccc ggcgccttca gccaacgttc
      301 cgccatcggc tccgtgattt cgtacagcga gtccttcacg aggcccttct tgatcgcgtc
      361 ctgcagcgtc aggaagaact cgtccattgt caggagcaca atctccggat gcgccgcgta
      421 ctcgtccagt tccgactcta tctgtttcca gatgccaggg gaaatctggg cgaaaaggaa
      481 catgaattgc ggtcgggtgg gacactcttc ccggaatttg atgatgtcgg caacggtatt
      541 gtgccctaca ttcgcgatgt gcacggaatg gagtttcggg atggggccat cgagataact
      601 cttcgcatac ggcgacccgc ccagcccgca caccagcccg ggaccctcct tcagtgcttc
      661 ctgctcgcgc cgcacggccg cgtcgtcttc cacctcacgc caccagtctt gcagatacca
      721 gttgaccatg ttgcagccat tctgtccgga ctgggcaagc aggcgccggc tttccgacag
      781 gtacagatcg acaagatgcg cgggccacag gtgagagtag gtgtagccgg caccggagga
      841 aggtccccag aagcagtcgt tcggtgtttt cgtttcgtgg aaaagctgca ccattccggg
      901 ctgaagtttg agcgcaagcg gcagcagccc ccatccgaac ttgaatttgc cccggtttgg
      961 ttccgtccag tttcctgact gcatgctgtt tgtcgcccac gtcgcatcgc catcgctgtt
     1021 gtacaggcag acgtacgttt tgtcgggctc ggcgacgcaa gacgcgcgct caggaagcgg
     1081 ctgatggtaa ctcctgccgg tgtctccgac acccccgtga atcgtcagat taggcgagct
     1141 catgctgcac aggacaaaga aaccccgctt tgcggcctcc gcgacgtatt ctttttcctg
     1201 gtcgaagacg cagtgccagt tcagtagcgc gcccggcgcg tgggcgctct caaggaggcg
     1261 gtgcagcaac cgcaaaacac gccgccgctg ccggctcgca ggtaggtcta tcgcgaagat
     1321 cctgttggcg acaacgtagt cttccaactc gtgcggaatc gagctccaac cgggacgatg
     1381 gatgcagaaa ttggcgatca ggcgctggtt gcactgcggc cagaggttgt ccactgccca
     1441 ctcggcggcc tgccagtcgt ctgaaaatcg tccgcgcagg tcatcccgtc tgggaatccc
     1501 gtgacgcagc atccacgatt cctggtcaac cgccaccggg aggcaaccct cgaggccggc
     1561 cagtgtgatg gcaaggttca tcgtctgcgg aacgtccgcc atatcataca ggatgtaacc
     1621 gttcacgcga tccctgaagc gctcgacaat cgcgtcgacg ctgttttcgc gttgcaatgg
     1681 aatcttgaac ggcgacgtat caggattggt gtaataatcg agccagtggc gctcccagta
     1741 ctcgcgcccc tgtttgtgaa cctcgcctcc cttgatcagg tagaggtgcg gttcagcccg
     1801 gttcacaagg ccctgcaacg agcggaacca gatccagaag tccaaccctt cgttctccag
     1861 atcgggcgcg gtggccaaga gccgtcttac tggaggtacc gtcgggaaga tatcgtcaag
     1921 tcccattgtt tctccattcg gtcagatcgt acgtggcggc cacccggtga attcctcttc
     1981 acccgaggcc cccagggcat ccgtcagctt gcgcgccgcc gcagcgactc cctcacaaag
     2041 gggagccaat tcggcaacgg agggcgtccc ggtctcccac ttgtcccatg cgtcaaacag
     2101 gcgctcgaaa ggggtcacgt cgcagatggc cgcgcagagc ctgcggaagt cgttcagaca
     2161 ctggtcacga tggttcgcat acacgccttg tgcctcgatc gccgcccgaa caaccaccca
     2221 cgcggttgtg aaggcgacgt agaggagtgc atccgaatct tcagtcgcgt caggcggctt
     2281 gcgccccttg aaacgggtca ggaacttttc ccgatccctc gcaacgccct cgatctctat
     2341 ggacaccaga tccgtccacg cggcatcgcc gatgtgccgt ctgcgctcgt cagagggcat
     2401 gcgagccaca tgagcaagct cttccgccag ccgttcgcac agcggaagcc ggtgccgccc
     2461 gggcgccttc agccaagtct ccgccaaggc ttctgttttc tcatacagtt cgtcgccgac
     2521 caggccccgt ttgaccgcat cctgcagcgt gaggaagaat tggtccatgc tcaggatctc
     2581 gatttccgga tgttttttgt attcctcaag ctccgactcg atctgttccc agatcccgac
     2641 gctgatctgc gcaaacagaa acatgaacaa tggtcttgtg gggcattccc gggagaaccg
     2701 tacaatgtcg cccacattgt cgcgaccaac attcgctatg tgcaccgagt ggactttcgg
     2761 aatcggtccg gcgaggtaac ttttcgcgaa cggcgaacca ccaagtccgc agacgaggcc
     2821 tggaccggat gccatcgctt cctgctcgcg gcgcactgct ttctcgtcct ctacctcgcg
     2881 ccaccaatcc tgaaggaacc agttcacgag attacacccg ttctggcctg tctggtcaag
     2941 cagtttccgc gattcggtca ggtacatgtc aaccaggttt gttggccact tgtgggtgta
     3001 cgtgtatgct gctcccgaag atggtcccca gaagcagtct gcctcggttt tcgtctcctg
     3061 ataatactgg agcattcccg gcatcaattt caccattaac ggcaggaaac cccacccgaa
     3121 tttgaacttg cctctctcgg gaacaagcca gtttcccgag tggaggttgt tcatcgccca
     3181 cgtggcatcg ccgtcgctgt tgtagaaaca gacgtacact ttcccatttt ccgctttgca
     3241 tgattcgcga gccggcaatg gctgtacgta ggacctgttc gggtctccga ttccgccgtg
     3301 tattgagagg ttgggcgtgc ctgtgctgca gaggacgaaa aaacctttcc gggctgcttc
     3361 ggcaacgtat tccttctcct gctcccacgc acaatgccag ttcatctggc agccaggcgg
     3421 gtcagcgctt tccatcattt tccggtagag atagagcgac ctcctgtgag ttcggcaacg
     3481 cggaaggtcg agcgccatgc ctctgtgcat gacaatgaaa tcttcgagct ggtgccccaa
     3541 agcataccag tttgggcggt ggatgcaaaa attcccgtag atttttcggt aacacgaggg
     3601 ccagaggtgc tcgatcgccc attctgcggc gtcccaatcg tcggcgaact tcccgcgcag
     3661 gtcgtcccgt ttcacaatcc cgtgacgttg catccagtgt tcctgatcgg gagacacggg
     3721 caacaggcat tcgaggccca cgcgggtaat tgccaggttc tgcgtctgga tgatctgttc
     3781 gttgtcatag agcacataac catcgatcag gttcttgtac cgttcgacca cggcatcgac
     3841 gctttccacg tcctcagtcg ggatcccgaa gcgcgtcctg taatagtcca gccagtgcgt
     3901 ttccgctgcg cccaagggtt tgtgcttctc catcgcattc aggtcgatca ggtagagatg
     3961 aggctccatg cggtttgcga gcccctggag cgatctgaac catatccaga aatcgattcc
     4021 ctcgccctgc ggattgatgc gcgccacgga gagccgtttc accgggggaa tcctcggaaa
     4081 gacatcggtc agctgcattg cggtgcctcc ttggggggag tattggcgga actggatttt
     4141 gcggggctgt tgtcaggaac gcgtcaccag ccgttccgtc cgcgtatcgg ggatgcggac
     4201 tcgttccccg tttctttcgg ctgaaagcgc tgcatagatt ccggggaggg tatagtccat
     4261 ggcggtgtgg acatcaattg gtgacggggc gcctcttcga aatgcgtgcg caaaatccca
     4321 cacgaggccc ccgtctatcc cgccatgtcc tgacgaagcc aggggcgttt cgactgcggc
     4381 gggccattct ttgcgctgaa tctcttgcat ggttccgtcg gcgaggtaga gcttcccgga
     4441 atcccacggc gcccttttgg attcggcgta cccgcgcgag cagtagaggc tgtaccactg
     4501 gccgcccggt tcttttggag cggagaagat gcaggactgc cgtatgggcg ctccattatt
     4561 cgtttcaaaa agggcgatct ggccgtccgg agacccgatt tccggttgtg tcctgcaccc
     4621 agtggaaagg caacttaccg aggtgcaacg gtcggaggta agccagagga gcggacctag
     4681 ggagtgcgtg atgtactgaa tggggttgta ccgggcacgc catgacggcc gtgccgtcgc
     4741 cgggcatcgg ggatcggcgg gattgtagta gcggccgtcc gcgtccaccc aggtgatccc
     4801 gcggatgtcg tgcacgtact ccccttcggt gtggatcacc tcgccgagga caccttcttc
     4861 gagcagcttc ttccatgcgt ggatgaaacc gtagtagttg cagttctcac cgagcatgta
     4921 atatccgctc gtcctcgcaa cggtatccac tatcctctgg gcatcatcga gcgtgtgtgc
     4981 agcaggaact tccgagagga ccgccttccc ggcttcaagg gcggcacata cgtgatccgc
     5041 atgactggga ggagcgcttg cgacgatcat tacgtcaaag ggctcccgca acatcgctgc
     5101 gtagtcacgg aaaccttccg cgcgagggca tgcggaacgg gcacggtcaa gccgttcggg
     5161 aatcgcgtcg cagaccgcga ccactctgta cccgggcacg cggtcaaaaa gttgtgccag
     5221 gctgagacca cgcccggctc caagcaaacc cacggttatc gtcgtcaaaa ccttcgacct
     5281 ttcctgactt gcttccggca aaatgcgtga ggggggtacg acgtgctcca cggcgtaccc
     5341 cctccttcca cgttattcca cgcagacgct gtcggccgga ggcgacatca tctcaccttc
     5401 catcaacgtg agtctgctgc acagcggttc acccgttttc ggatatcgca gcacgtcgaa
     5461 gaactggata gcactctcgt acggcaggct gtcgtacccc cagaacccga tcaggcgctg
     5521 ttgtggcgga gggatgtgcg ccttcccgtc cattgctgtt ctcagtttgc cgcggagtcc
     5581 ctcgatatcc ggtttcccgt tcttcgcagg aagaacgtgg aatgtgaagt taccttccag
     5641 cgaatccggc cacacgaccc atttgatcct ctgatcaggc agccaccaga tccaggcggc
     5701 ccatcccatc gggccaaact gcggatgcat ccgcggaggc tctacggagt gctggttacc
     5761 ctcggccgtt ataaggaatg gcatctggaa agttccgttt acgcctatgt agatgcccag
     5821 catcgcttgc ccgtacgttg agctcttatc aagaacgaga ttgaacgcat tgggcacccc
     5881 gcggggtccg ggattctgcg caagtgtctg gtgccccaca cggagccgat tcaccatttc
     5941 ctgcacgcca agttccgttc ccgcgtcgat tttcttcgtt gggtcgatgc ttaccagccc
     6001 ccgaacagaa tcgcctacca cacgcatgaa aatcgtcggg aacgcgaagg ctgaatccgt
     6061 gcttgcactc gaaggcggtt gatacttctt ctggccggtg agctgctcct tcagggagcc
     6121 tggtcccatc aaggacttga taccgaaaaa ggcaccgaca acgactatgc agattgcgat
     6181 gcccagtcca atcccttgga ccattttgtt ccgaaagatg ttatccatgg aactttgaat
     6241 cctctcagat gtgagtggac agaagcgtcc tgccggccac tgaggaaggc gattccgggt
     6301 cgtaccccga tgcctgcatc aacgggagga tgacgggaaa acgtttcaaa tatagcttga
     6361 gcaagggtgg agacacaggt ccccgggtat tctggctaac gcgcacgctc atcaacgact
     6421 cgctgcagcg cggaaactgc caacagatgc cacatgttgt gcaccaacca cggctcgctg
     6481 gttcggtaag acgggtggtc acgttcggtt cgggatcgcg acatatcgaa accgggctgg
     6541 tcaagcgctc tgggggtgcg gacgaagcca ttcggtatcg cacccggaac tgcaccgcgc
     6601 tcacggcccg gaatggaatc gtaccggtgg tggtaccgcg gagggttgtt acttccggca
     6661 ccttccatca tacagagccc gtaggggttc ttgcccatca cccagtcgat ctggtcgagc
     6721 gcgaacgtca gtgccgcctc ctgtccggtg gcctggtatg ctattatcgc ggaccatgcg
     6781 cgggcaagga tttcgaaatt gtggccgaga gttgatgtcg gctcgaagaa gaaatcctgt
     6841 tcgccaacgg attgcttcgc gagcccgaaa gggttctccg ctgtcgtgag gcagaactct
     6901 atgtatcttg ccagagcagc ccgtatgcgg ggaattcgga aatcctccgg gaacttgagc
     6961 gcataggtgc ccagtgcccc cgccgcgtgc tcgccaaatg ccccgaacgc acccgcgtgt
     7021 ctcccggagg ggacctggct cgcaaggagg gtatccacgc accgggacgc aaattcagcg
     7081 taccgtcgtt cgcctgtcgc cgcgtgcaga tcgagggcac tgagcagatt cagcggcgaa
     7141 aaccccgttt ccccgttctg cgtcgaatac ccccataacc gctccgcgct cgacccgtac
     7201 tttgacccat cctctccgcg tcgttccaac tcccctgcaa gccgcatcca gccgccgatc
     7261 gcaagcgggg agttcccctc cccgggcaaa atgaccgggt cgtcatcggt acctatgatg
     7321 ttatcagtgt gaacctcagg tggacaccac ttcatccaca ttcggcccgg cccctgggat
     7381 acatccttcc gaagaccgcc ggtttccggg acttgcattt tcacgacgaa atccgcaccc
     7441 cagcgggcct catcgaggat atccggcacc ccgtcgttgt ctctatcgga gttctcaaaa
     7501 acctgctctt tggtccgaaa tgcttcggta agggcataaa gcacgccgcc gtcgccgttc
     7561 tcgtacatga gtttgttgta atcgccggcg ctgtgccatc ctccaacggc gtcaatatgc
     7621 gtgccgtcgg gaaggagcgc gtcatcgagg tggcaggccg catgccagcc gggaaccgca
     7681 aaaccgcacc tttgaacaaa gaaaaactcg acgccgagtg gagccgtccc gccgaagaga
     7741 atgccgtcgc cgatcaggaa cgggtacgac tcacctcgca cgccgccaac cacagccgta
     7801 atggtgcagc gcccggtctc tttcagagac gagaagtccg cgcgccagaa gtattcgccc
     7861 cagtcgtccg gcatcccgtt gtgtattcgc tcaggaccga caagaggaaa acttccgagc
     7921 gtgtggccgt cctctccatg cacttcaatg aaattcctcc ccccgtgcgg aacgaagttc
     7981 gtcgcgacaa cgcagctttt cggcaggttg cgttcgtacc cgacctggtt cacatgaact
     8041 ttgaccacgc ggcgaaggtt tgtggccggc gcaaccggtt ccagttggaa gtcatcgaac
     8101 cacgcagtcc cgatgtttcc aacaagcgaa accgtcatcg tttccgcctg aaccggggga
     8161 tgagcgtcgc tcacctgaaa acgggtccat ccgttcaggg tttcgcaggg agttgcgggg
     8221 ctcagcccgt gcagaacgcc accgttatcc gtccaggcga tggcgatatc gggggctgct
     8281 ccttcccagc gcacccatcc cgagagccgg aacgttgtca ggccaaccgg aatcggttgc
     8341 tgaatattcc gcatcgccca ccatgaggaa tccgaccgaa tcctgagccc cgcgccgttc
     8401 cgcccaccgg tttcatcggc gatgcctgaa ccggtttttg cagtagccct cgtaaggacg
     8461 gggtccggat cccagaaggt cacgcgtccc ctgttgccgg cttcgaacgt tgggttgtac
     8521 agcagattgg cgatgagcgg cgtcatcacg atgtcatcac agagcgcttc tccgcgaacg
     8581 atgagactca tgcggacgtg ttcggccgaa gccggcgcat cggtttcgac cgcggtgtac
     8641 cgccatccgg atgcgtgtcg gtctttcgcg atctcagggg tatccgcgga tccgagcacg
     8701 gcaccggcgg aattgagaaa ctcggtacgg aggaagacca gccccttctt cacacgaaac
     8761 catccgctga tcctccagct ctgtccagcc tccacctcca tgacgtctga cgtccacacg
     8821 gtggcctgtg tccgagccct tcccccaatg ctcctggtac cggtatgtcc atcctctcca
     8881 aggaagccac cggggcgccg gatccagccg gaaggggatt ccgctccatc ctcgaatgat
     8941 ccattcaggt tcatagtcgc actcgctggg ttggcgctca tgacgatgac aacacacaaa
     9001 atacttggta ccaggcgggt ttgtatgcaa ccggtcatac tgcgcatgcg aaagtgctcc
     9061 ttcagcggcc cgagttcgtg acgttttgac tcccaaaaag gaaccgagcg gggtgggaga
     9121 ggaccaaccc tgccggctat atttggtcaa cgatgcgtgt tcaccactcc tctcttcggt
     9181 tgttccctcc gatgaccatg cgtgagcgaa ttctggcagt aattcgcggt aacccgcacg
     9241 accgggtgcc tttcgttcaa tacgacggtc ttgccgcgcc gaacgaggtt gtctgggatg
     9301 ttgtcggtcg ggcgaacatg ggcatcatcc gttggtcctc cgcataccgg atcgaagcac
     9361 ctgattgcgt tattacgcac gaacctgtcg agatcaacgg gagaaccggc gaacgtgctc
     9421 gcctgattac accgcgggga tcacttgttc aggagcgggt gttcgaaccc gcatatcagt
     9481 ccggcgccac gcgcaagcac tacgtgtgcg attccgccga ctacgagatc ctgctttcct
     9541 acctgcgcaa cgtcgtcgtc gttgatgatg cgacaccatt tctgctcgac agggatgcgc
     9601 tgggagacga cggcgttccc atggtggcgg ttcaccgcac gccttaccag cagatgtgga
     9661 ttcagtgggt atccctcgag cagctcgccg ttgatcttga agatgacctg ccggttctgc
     9721 gggagtgtct tgaagttctt gggaacatcg agcggcggat attcgagtcc gtttgccggg
     9781 cgacgcgcga gcacggagtt cccttcgttg actttcctga caatatcacc gcaccggcga
     9841 taggtgaacg gtatttccgc gggtattgtg tgccgttcta tgacgagctg gcaggcatgc
     9901 tcagagacga cgacgcgccg gtgttcgtgc acatggacgg agatctcaaa ccgctctggg
     9961 aggccatagg agggagcggg gtccggggaa tagactccct ttccccgcca cccgataacg
    10021 acacttcgtg cgctgacgct gcaacccgtt ggccggatat gcgccttttc gtgaacttcc
    10081 cgtcgtccgt gcatctctgg gaaccagaac agatctactc ccacgccatg gccatgcttc
    10141 gacaggcagg acacaccggc cgcatgcaga tacagatttc cgagaacgtc ccgcctggcg
    10201 tctggcagaa aagctttccc gtgattgtcg aagcggttcg agatttcgga aagccctgat
    10261 tggatagggg aaatcccaat ggacacgtac tcttttgccc ctgagttgtg ccacgagtat
    10321 ttgatggaca gggccccgct cgcgctcacg ttcgacccgc acaagcattc ggacgccgaa
    10381 tccttcgacg cgtggcggcg caaggtgggc gcgaagctgc ggcaacttct gggaaacacg
    10441 ccggcaaagg tccccctcaa catccggcat gaattcgaga cgcaacacga ggaattcgtc
    10501 gagcggcggt tcgtgttcac ggcggagaaa aacgccgacg ttccttgcca cctgttgctt
    10561 cccacacacg gctctgcacc attccccgtc atgatttgtc tgcaagggca ctctacgggc
    10621 atgcacataa gtctcgcgcg tccgaagtac gagggggatc agaagtctat cagcggcggg
    10681 gaccgcgatt tcgccattag agccgtcaag gaaggattcg ccgccctggt gatggaacag
    10741 cgctgtttcg gggaacggaa agatgcgaaa catgagggct cccggtgtca ccatgccaca
    10801 atggtatctc tgttgctcgg aaggacgatg atcggcgagc gggtgtggga tgtctctcgc
    10861 gcaatcgacg cgttggcaac cttccccgag ctagacagcg acaagatcgc ctgcatgggc
    10921 aattcgggcg gcggaaccat cacgtatttt gccgcctgtt gcgatccacg gataaaggcg
    10981 gccatgccct cgtgctacgt gtgcacattc cgcgattcaa ttggtcggat ccaccactgc
    11041 gaagacaact atgttccggg gattttgaag tggtttgaaa tgggcgatct cgcgtgcttg
    11101 atagccccga ggccattggt ggtggtcgca gggaaggagg acgaaatctt cccgatcaaa
    11161 ggggttcgga gcacttttcg gcgcattcgc gatatctatt ccgcagccgg gcacaaagac
    11221 aaatgtcggc tcgtggtggg aaatggggga caccgattct acgctgatga cgcgtggcct
    11281 cgtttcaaac aactgacgcg gtggtaatgg cgtcgcagtt caggacgatt ttcttcacaa
    11341 tggaaagggc gaccaggtga ccaaagtcgc aatgatgggt gcgggcagtg cagggttctg
    11401 caggatgctg gtcgaggaca tactgagctt cgaaagcatg aaggacatcc atttcgcact
    11461 gatggacgtg gatccagaac gtttggatct cgtgtaccga tccatgctcc ggatgaaaga
    11521 ggagcatacg ctcgcgtgca cgttttcggc gactacggac agaagagaag cattgaccgg
    11581 agccgatttc gcgatctcga tgatccaggt aggtggtctg gaaccgtaca agctcgacgt
    11641 aagcattccc ctcaaatacg ggattgacgt ctgcgtgggc gatacggtga acccaggcgg
    11701 gatatttcgt gggctgcgcc atgtacccgc tcttctggac atgattcgtg acatgcagga
    11761 tgtatcgcac cctgaaatca tcttcatgaa ctacgccaat cccatggcga ttgtgtcgtg
    11821 ggcgatgcag aagatgtacc ccgacacgac gtcggtcggt ctttgccatg gggttcagca
    11881 cacgaccaat atgctctgcc agtggctggg agtggcagta gaggagtgcg acgtcctgac
    11941 tgccggaatc aatcacatgg cctggttcct taagttccgc cacaatggaa gcgaccttta
    12001 tcccagaatg tgggacaaac tcgccgctga aggccccatc cggggcgagg agtaccggtt
    12061 cgaaatgatg aaggcaacgg ggtacttcat gacagagtcg tcaggacatc tttcggaata
    12121 cctcccctac ttccgaaaac ggaaggacct gctcgaattg ttcaacggcc cgggtttctc
    12181 aggggaaaca gcctattacc tgaacagctg cctgcaacac cgtgacaaat atctggaaga
    12241 gatgcgtgag ttggcggacg ggagaaaacc cgtaccgttc cgccgcgggt acaagtcggt
    12301 cgagtacgct tcaaacatca tgaacgcgaa gcttaccggg gagattacaa ggttcgcggg
    12361 caacgtgctc aacaaaggct tcatcaccaa cctgccttac gattgctgtg tcgaagtacc
    12421 cgtgtttgtg gacaggcttg ggctgcacgc gtcgtacgtc ggtcatatcc cggagcagtg
    12481 cgcggcgctt tgccagtcga acatttccgc gcaggagctc gctgtgaagg cagccatcaa
    12541 cggcgatcct gaagccgtct accacgcgtg catgcttgat cctcttaccg ccgcggtgct
    12601 tgccccccac gaaatcagga acatggtcga tgagatgctc gaagcgcagg ctccgtggtt
    12661 gccgcagttc aaagggaagt cgtcacacgc gccggggcac cgggtcggca gactccagac
    12721 cggggcaaaa tcgctggttg tgggagccga ggtctcttca aagaagggac attacgactg
    12781 accacgacaa gctggcttga tcggcatgac ataatccaca ttatcagtat accatcttcc
    12841 cattacggag aatcttatgt cacgcaagat tcgcgtcgct gtcacgggcg ctggtggcga
    12901 agcccgttgg tacatggagg cgtacaagac ctgccccgat tccgaacttg tgttggtaca
    12961 ggatatcgat cccgcgaagg cacgtgaaca gggagaacga ctcaacgtaa actggacaac
    13021 gacgtttgag gacttgcttg gccccgacat cgaccttgtg gacgtcagca cccccaatca
    13081 cctccacgcg caacagacaa tcgccgccca cgaggccgga aagcacgtga tttgtcagaa
    13141 acccatggca cccacgatcc gtgattgcga tgaaatgatc gctgcggccc agcgcaacaa
    13201 tcgcaaactg ggcctttaca tgagttctct gggtgccccc ctcttccacg agatcaagga
    13261 gatgatccga caagggttct tcgggatcat ttcctctgtc agcagtcgcg gagcccatcg
    13321 gggcggatat gaggcgaaag acaataccgc gtggcgaggc tctgcggaga agaccggcgg
    13381 cggcgtgttt atccagcttg cagtccactc cattaatctt ttccagtggc ttctggatga
    13441 cgacatcgtt caggttgccg cactttccac gaaccgcttg tgcaaacatt cgatcggtgg
    13501 ggatgatctg acccatgctg ttgcggaatt ccgcggaggt gtgtacggtt cgttcgaggc
    13561 ggggtacgcg gctgagggag ggctttttgc cctttacgga acaaagggat ctctggtgat
    13621 gacggcggga cagacattcc tgtggtcaga gcttccctgg gacggcgata tcatcaagta
    13681 cacccccagg ggtggcaaag agatgacggt gctcgacccc gcagattacg ggccacggca
    13741 ggccagattc gccgggaaat acgaccagca tgccgccttc gtccgtgcga ttcttgagga
    13801 cgctccggtc cctgtgcctg gcgaaattgg acgtcgtgac gttgccatcg tcaaagcagt
    13861 ttatcgttcc gccgaggagc ggcgtcatgt gctcatcgag gagatgggat agctgaatat
    13921 cccaataata cggacaaaga cttttttctt agggagtgga cagtgcggca cgtttctttg
    13981 tacgacctcc ccgatggaaa tgaccaggcg gagctcctgg ccaatctccc tgcgtcctac
    14041 gggtcgttcg gtgcgagcgt caggttcttc gctcgcgggg aggccaccga ggcgcaacgc
    14101 gcatcgaacg gagttctcct cctggtggcc cagggaagtt gcgttgtacg ggttgatgaa
    14161 accgaatatc cggccgcaag gttcgatctg ttcgcattgg aatcaggtga aacattcgaa
    14221 atctgtgcgg atgagatcga cccggcggtt gtgctcagaa ttgatgccgg caattccgag
    14281 ggttaggaga gtacgtgttt tttggaagcc aggatttctt catactgttg ttgatagcat
    14341 tcggtgctgc agtggtccag ggaacgctgg gttttgcctt cggtatggtc gccatggcac
    14401 tggctgcgct cgtaatagat gtgcgaactg cttccattct cgtttccccc ttggcgtttg
    14461 tgaacgtatc caccacgctt tggagcgtgc gcagaagcat ccggtggaag cttgttctgc
    14521 cagtcgtcgg cggtacgctc gttgggattc cgttcggtct cgcgatactt ctgggcggca
    14581 acctgatcct gatccggttt ctggtcagtg ccctgcttgt gtatgtgggc gtgaccagcc
    14641 ttattgggtt caaggtgcga aggcacgctc tgccgggctg ggtcgccgcg gtggcgggtc
    14701 tcggtgtcgg ggtaatcggg ggcgcaacga acataggcgg ccctccactg gttgcatatg
    14761 cctctcgcca accatgggat gccgaaacgt tcaaagcgac gcttctgacc accttcttcg
    14821 tgagttcggg aacaaagagc gccttgttga tcgcgaacgg agcgattgac gctcgcatgc
    14881 tggcgtgcat cgcagggctc gttccggcga tcattgccgg atcattgatc ggcgtgcacc
    14941 ttttcaacag aatcaacaag gaaaggttca aacgaatcat ttcctggaca ctgcttgtcc
    15001 tcgcagtgtt ggttcaggta tagccagaca ccacggacag ggagccgcga tcagaggtgg
    15061 atatcgacgg aaccacacgc gttgttggcg taattggcga cccggtaagc cacagccttt
    15121 cgccagcgat gcataacgcc gcgttcgaag cgctcggatt gaactgggta tatctcgcgt
    15181 ggcatgttgc cccggaaggc gtggaacaag cggtgaatgc cgtccgcggt ttggatctgc
//
LOCUS       DAOSOO010000060        15234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3922_length_15234_cov_5.594571, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000060 DAOSOO010000000
VERSION     DAOSOO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15234
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_15234_cov_5.594571"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLT86_06075"
     CDS             complement(<1..662)
                     /locus_tag="PLT86_06075"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOT36212.1"
                     /translation="MVPVRVAVTGAAGQIGYSLLFRIASGEVFGKGLPVVLQLLEIPQ
                     AMKALEGVAMELEDCAFANLAGVALSDTPEQAFSGANWALLVGSKPRGPGMERIDLLR
                     DNGGIFVRQGKALLNAASDVRVVVVGNPCNTNCLIAMAHAGDIPPERFSAMTRLDQNR
                     ATAQLACRAGVPVGQVTNVTIWGNHSTTQYPDFEHARINGDPAETVIRDRSWLENDFT
                     ETV"
     gene            complement(792..3674)
                     /locus_tag="PLT86_06080"
     CDS             complement(792..3674)
                     /locus_tag="PLT86_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOT36213.1"
                     /translation="MSILESFVALDFETTGIDPFSSEIIEVAAVRVQSGQIVDSFQTL
                     VQPHKMPSVSIQRLTGITADELQGQPSLEGVMGRLRRFVGATPLISHNADFEVSRLRM
                     SIPAAELPTVFDTVELARIALPRLPNHRLETLVDAFSIENPEQHRALGDAQTLVEVFR
                     ALVNTMRSFSPARLDTLAKLARSLPRPEPPFGAIADLLLDIQAAAARSAFDQVSAPPL
                     QVGALTSLPNTFGAFDDNRLEPATSLEDIDAESIAETLGAHGDFARAMPTYEVRRSQI
                     DMARAVTEILNEGGTLLCEAGTGTGKSLAYLVPSVTWALKNSHRVVISTHTKNLQEQL
                     FYKDLPLVAGTLDVPVRSVVLKGKNNYVCLHQWHEEIGLPPAEAVDASRALLLPLVCW
                     IDETESGDLEENGAFQTLLRYVPGAYSLAVRLSSEGKPCGRRTCRYYDRCFVTRVRRA
                     AQQAHIIVVNHSLLFSDLAADHGVIGEYENLVVDEAHTFEETATDYLGIEFSLPLVRS
                     VLDEIITPHARSLLTRIRGNLRRSKIEDSTRKSFEVQLGATGQAAELAGEKAEAFFRA
                     AKNELGAPHPSSIYSSKMRYSSEVNVFNEIREHRDDLSFALKTLHDGLQTLALWLRDL
                     SSGGVPEQDELLADTELRQADVARLTAAMDLITRAGDEGMVYWSEMPPHGDETRLKFI
                     AAPLDVGATLRRDFYPSLRSLVMTSATLSVAEKFNYFRQRLGLEDDSVRTMAVKSPFD
                     FENQVHIALPGWFPEPRDSAFEPEVISLLRVLIPRIHRGTLVLFTSHGMLENAYNALK
                     QPFEEQGILLLGQRFDGSRSSITNRFRQDRQSVLFGTESFWQGIDVPGEALELLIIVK
                     LPFSVPGEPLETAQSEALAAAGKNPFLHLAVPKAAIRFRQGFGRLIRHRNDKGAVLIL
                     DTRLVSKDYGRAFHRSLPASYTVYRSQEELIAGFEAWFAAEISTKL"
     gene            complement(3671..4231)
                     /locus_tag="PLT86_06085"
     CDS             complement(3671..4231)
                     /locus_tag="PLT86_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36214.1"
                     /translation="MTWLERVGAFLAHRRVQSMAAEPVEIREALRAGGKTLVFLPQEP
                     ELLDSGVSICVTLAGWFGPLALCCVSRDDKLPDLSNSGYPAVLFPWAISRMGLPTRNV
                     REKVKVLAPTIAVDLHPQFNLPTAYLATESGASLRIGFGDAPSGYFNLRYACPSFDPA
                     NSALHYGRFLRTIENLRADTFEEPPR"
     gene            complement(4228..5112)
                     /gene="pheA"
                     /locus_tag="PLT86_06090"
     CDS             complement(4228..5112)
                     /gene="pheA"
                     /locus_tag="PLT86_06090"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOT36215.1"
                     /translation="MVVVAFQGELGAFSEAAARAYFGNGIQLSPSATFRDVFLKVSTG
                     EADRGVIPIENSLAGSVHENYDLLLEHSLHIVGEVKLRIVHHLVANKGVALPDVRRVF
                     SHPQALAQCQEFLRGLPGVTPVPVYDTAGAAKELKASGMRDAAAISSAQAAMDYELEI
                     LASGIESNHQNFTRFLIVALQPDADRDRPGNKKVSVAFALHHAPGTLFKSIGVFALRD
                     IDLLKIESRPIPGTPWQYLFYLDFAGDVEEPRVGKALDHLGELTSFLRVLGCYPIGDT
                     IDSATPFARDRAGQDSER"
     gene            complement(5114..7162)
                     /locus_tag="PLT86_06095"
     CDS             complement(5114..7162)
                     /locus_tag="PLT86_06095"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOT36216.1"
                     /translation="MILQNDLIRVELGDETGGFRSLKDLRLDREWVCAPDRSLLFRLM
                     VPDGERECEHVDSDVPAVVPGGSSCVLTFARNGISMQAFLELDGDALVCRLQIVNDGP
                     APVEEIMFPYVRGLAPVEKACITWPHFHFRRIDDVFGRGLGGDHHSWNEMAQKYVGRY
                     PHWLTTAWCDYGNDSSGIALEGRHKDFSIMDFCLHKVVEKHFDPVRRSLDLYTVHPHR
                     VAPGETFTTPPVRIRLHEGDWHTVADEHRGWLDTWIGKPERPAQFAESIGWHFYFMKH
                     QDGLEVNSYADLPRMAKSALAAGCRYLMVFGWQTGGHDNNYMYRYVPNDSWGGEAALK
                     AALEECRQLGVQVIPFFNGTLANTQMPEHREYGYRWEAITRAGHPYYAGDWARNNFDA
                     PSRNRSMLHHEVCFCEEYRPHFLDAVTRIVKDYGFGNTQLDQIAEKMFVCYNPSHGHG
                     APDRAFVDGLAALLPGVRKIVRDANPDGVIVGEGFNDFVNQWCDSSWTWRGLFDFPEA
                     VLFSLPWLFASCEIDALEYGDANRAFAYKLQLDMKIDGGDGNISDYPEFCSFIKSHAE
                     LRSRVAPYYVYADFRDEENLAVRKPSGVFAKTYLNRNEGKVGVVIAETAGKPADVEFA
                     HTWPTDERAARVWSSQGEMSQITVEGVVRMELRPFEVLVVCLDGVRTHHLPNVRKDLS
                     "
     gene            7256..8101
                     /locus_tag="PLT86_06100"
     CDS             7256..8101
                     /locus_tag="PLT86_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOT36217.1"
                     /translation="MIKTSTYPCKMIAKDIPGAEGPCVNMRGEFFMVAPNLGQVFRVN
                     ESGETSIFAETGGIPAGLQCDRSNRLWLADMKRGILSISPEGIVRVETPEFDGKPMRG
                     CNDCAFDSRGNLYVTAPAGSGKDAPNGEVFCRKNNGELVLLDGGFQFCNGIAVNADDR
                     ILIVAETLTRWLWAYDILAPGKVANKRKWAQLVDVEGGLGPDGLDFDSAGNLLAAYWG
                     GGSLDVFNPAGELIERIMLPFKAPSNLHFGGRGSRTIYITEHDSNGLWKLEWERDGQP
                     QFCER"
     gene            8159..9229
                     /locus_tag="PLT86_06105"
     CDS             8159..9229
                     /locus_tag="PLT86_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36218.1"
                     /translation="MDCAPVVIPYHAIFLRDHWEEVTTEPWWKTSDGNISSSVQVERD
                     FLESTGMDWTGVGIGASRQFRETHAASVRDGVPYLTNTVTGEETELRHWPRSGDHYSV
                     NCPSVTNLEDIERVVQVRSAREILDAGFLDGARAVVESVGASHAVYAAVNHPSWTYTC
                     FGFENMMTNMVENPALVHALSERMCEKAIEEIKAFAAIGVDFVWVELCLVGRDSISLS
                     AFREFVTPYSRPLVDVARENGMITVFYVCGDVSDRLEDLVSVSPMCLSFEESKKGFCV
                     DLDTISRVVGDSVCLFGNLDAVDVLENGSDALLAREIERLLEIGNRTGRFVMSLGSPV
                     TPDTPVARVRRFGELTRQFSSR"
     gene            9290..12019
                     /gene="polA"
                     /locus_tag="PLT86_06110"
     CDS             9290..12019
                     /gene="polA"
                     /locus_tag="PLT86_06110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT36219.1"
                     /translation="MSDAKRLYLLDGTALVYRAYFAFIRNPLINSKGENTSAPFGFVS
                     ALLKIVREEAPDYLAVVFDSAQPTFRHERFPAYKATRERMPEELVAQLPRIDQIVEAF
                     GVPIVRLDGFEADDLIGTLAVQGQNAGLDVTIVTGDKDLMQLVNDRVRILAPGKTKNE
                     WERIGPEEVVARWGVSPEQIRDLLGLMGDSSDNIPGVPKVGEKTAVELLKKYGSLDAV
                     LEAAPEIQKPMVRQNLMDFALQARLSKELATIAVDAPVTLDLEAFTWVAPDPARVVPV
                     FRELEFNQLIEQLGVQAPGPANKLPLEFRTVTADMLPGLCEQIRAAGAFSFDTETTST
                     DPRSASLVGISLACDGLTGYYLPIGHRNREPDGLAFTRSAESPVNLPLTTVKEHLQPL
                     FADTMLLKIGQNTKFDLAILNRAGFSVDAPLFDTMIASYILDPSARHGLDAMAEEHLG
                     HRMIPITDLIGKGKDEITFDLTPVDKATEYSGEDAVITYRLGRLFEERLKTNGLDELF
                     RTLEMPLVPVLLQMEETGIRVDAEALRDLSREISQQLHELEEQVYDAAGEKFNINSTQ
                     QLAHILFDVIGLPARRKTKTGYSTDVDVLEALAPLHPLPKLVLQYRSLAKLLSTYVDA
                     LPKLVNKKTGRIHTSFNQTVTATGRLSSTDPNLQNIPVRTELGGQVRRAFVPEKGWLM
                     LSADYSQIELRIMAHLSEDTTLIKAFQNDEDIHTLTASLVFGVPPAQVNRELRAQAKT
                     INFGVIYGQTPFGLSQQLGIPQREAALFIENYFKTYPGVKKYGEVTIERARKDGFVST
                     MLGRRRFVPDIDHQDVNRRRFAERTAINTPIQGTAADMIKLAMIRIHERIAAEKLRAR
                     MLLQVHDELLFEVPPDECDALKDLVRAEMSGALELRVPVRVDIGVGENWLVAH"
     gene            12051..13316
                     /locus_tag="PLT86_06115"
     CDS             12051..13316
                     /locus_tag="PLT86_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT36220.1"
                     /translation="MPSRNSVLSGVFVLGVLLLAGCDEITRSGDPGGATGAADPRLAK
                     EAVPPALVDANMRFAFGIYQRLVETPPAENLFISPASISLALAMAYNGAAGNTKEAMA
                     RTLGLGEMTLEEANQANLVLLSNLVYADETAKLAIANSVWANQGYGFYESFLDRNRRY
                     FGAEVATLDFARPSALATINGWVERNTFGKIPSILDEIPDFAIMYLINAIYFKGEWTN
                     KFDVAETRMRPFTLSSGSAKNVPMMTRHGRYAYLQNDLFQGVALPYGNKRFSMYVLLP
                     KEDVGLAALNRALTVSNWTTWVAAFDTMEGTISLPRFEARYEETLNSVLSAMGMGVAF
                     DPYGADFSAMLSVSPSRNVFINEVKHKTYLKVDEEGSEAAAATSVGFGVTSVPVTFNM
                     VVNRPFFLVIRENKSGTILFMGTIVDPSS"
     gene            13458..14033
                     /locus_tag="PLT86_06120"
     CDS             13458..14033
                     /locus_tag="PLT86_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36221.1"
                     /translation="MALQKTIRTAFLSLGVLGAAMMSGCILSNEDEAGTVKDHAGPNT
                     PVTVENAQTVFNMVFGTIKGAMEEPLSKPSAAASETRTINGSASGKIVVKTTPTANGY
                     TEELEITDFSNDGELFIGGKLTNDMTTTEHGETGNITGDIAVAGFFKGKVGVDVMMEN
                     FGDYFMVQGMVTVGGEEVSVFFHPTYTKVAR"
     gene            complement(14057..15172)
                     /locus_tag="PLT86_06125"
     CDS             complement(14057..15172)
                     /locus_tag="PLT86_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36222.1"
                     /translation="MALNGTVIRTGTIAVLLCSVILCVFTYLAVLADPSIPLLFAWRK
                     LRLFPWLADLRLNPVGTFVQTTGKAIAGLVLLYSTGAQVKTLFRHAKGDVSFSAHEAW
                     HSGRANSHALVTAIALLILVATGTLLSALALGLPGRIPVAGEIFAGFGFLLALPLVFL
                     LLVCLALIAGTLTVGVAIANTLDDDWLGTLVNVGALLWSQWWRFLVYEGIVFCITIAS
                     WLAATGLLFTCTGLAIVLVGLTTQGRLAAVAYVASQFVPGLDWVLSRVVPQFDVWSIV
                     VGPGVAVGGAFVSFGILFLLAVVTGYAAGVWSAGQMATYAELRRHQYSQDIYEKEDMI
                     DRVAALAPTLKSGRHEAGVIAPSGERKDAGDQKPPAS"
BASE COUNT         3344 a         4405 c         4356 g         3129 t
ORIGIN      
        1 accgtctcgg tgaaatcgtt ctccagccac gagcggtctc gaatgactgt ctctgccgga
       61 tcgccgttaa tccgtgcgtg ctcgaaatcc gggtattgag tggtgctgtg gttgccccag
      121 atggtgacgt tcgtgacctg acccactgga actccggcac gacacgcaag ttgcgccgtc
      181 gcgcgattct ggtcaaggcg cgtcatcgcg gaaaaccgtt cgggaggtat gtctccggcg
      241 tgtgccatcg cgatgaggca attcgtgttg catggatttc ccaccacaac gacccggaca
      301 tctgaagcag cattcagtag cgccttgccc tgtcgtacga atatgccgcc gttgtcccga
      361 aggaggtcaa ttcgttccat tcccggccct ctcggcttgg agccgacgag caatgcccag
      421 tttgcgccgg aaaacgcttg ttccggggtg tcggagagcg cgactcccgc aagatttgcg
      481 aacgcgcaat cctcaagttc catcgcgacg ccttcaaggg ccttcattgc ttgggggatt
      541 tccagcaact gaagcaccac aggcagccct ttcccgaaaa cctctcctga agcaatgcga
      601 aaaagcagac tgtagccaat ctgaccggcc gcgccggtaa cagcaactct gacgggtacc
      661 atttgacact ccttcacaac gatcaacttg ggacattgaa aaacgtagtt ctaccggccc
      721 gcacgtcggt gagttcggcg gaaccgtact gattgtccgc gatcgagaca atcatcaccg
      781 aaaaggcctc ttcacagctt cgttgagatt tcggcggcaa accacgcttc gaaacccgct
      841 atcagttcct cctgagaacg gtacacggtg taggacgcgg gcagcgagcg gtgaaacgcg
      901 cggccgtagt ccttgcttac cagccgcgtg tcgagaatca gcactgcacc tttgtcgttg
      961 cggtgcctta tgagtcgtcc gaagccctgc cggaatctga tcgcagcttt cggaacggcc
     1021 agatgaagaa acgggttttt gccggcagcg gccagagcct cactctgcgc ggtttccaag
     1081 ggctcgccag gcaccgaaaa cgggagcttc acaatgatca ggagctccag cgcttctccc
     1141 ggtacgtcga taccctgcca gaagctttct gtgccgaaaa gaacgctttg ccgatcctga
     1201 cggaaccgat ttgtgatgct ggatcgtgaa ccatcgaacc gttggcctag aagcaggatg
     1261 ccctgttcct caaacggttg tttcagggcg ttgtacgcgt tttccaacat cccgtgggaa
     1321 gtgaacagca ccagcgtgcc gcgatggatt ctgggaatca agactcgcag cagagaaatg
     1381 acttccggtt cgaaagcaga atcccggggc tctggaaacc acccgggaag ggcgatgtgt
     1441 acctggttct cgaaatcaaa cggtgatttc accgccatcg ttcgcacaga atcatcttcc
     1501 aggccgagcc tttgtcggaa gtagttgaac ttctccgcga ccgaaagtgt tgccgaggtc
     1561 atcacgagcg accgaagact cgggtagaaa tccctccgca gcgtcgcacc aacgtccaga
     1621 ggtgctgcga tgaacttgag tctggtttcg tcgccgtgtg gtggcatctc ggaccagtac
     1681 accatgccct cgtctccggc acgggtgatc aggtccatcg cggccgtcag acgagcgacg
     1741 tctgcttgcc gcagttccgt gtcggcgagc agctcatctt gttcgggaac gcctcccgaa
     1801 gacaggtccc tcagccacaa ggcgagggtt tgcaacccat cgtgcaacgt tttcaacgcg
     1861 aaagaaagat cgtcacgatg ctcccgtatc tcattgaaga cgttcacctc cgaggagtat
     1921 cgcattttgg acgagtaaat agacgaaggg tgaggcgctc cgagctcatt ctttgccgca
     1981 cgaaagaacg cctccgcttt ttcacctgca agttccgcgg cttggcccgt cgccccgagc
     2041 tgaacctcga acgacttcct tgttgagtct tcaatcttgc tcctgcgaag attgcctcgt
     2101 atccgcgtca gaaggctccg cgcatgcgga gtgattattt catcgagaac ggatctcaca
     2161 agcggaagag aaaactcgat ccccaggtag tcggttgcag tctcctcaaa cgtgtgcgct
     2221 tcgtcgacga caaggttctc atactcgccg ataaccccat gatcggcggc gaggtcgctg
     2281 aaaagaagtg agtgattgac cacgatgatg tgagcctgct gagctgcgcg gcgcacacgt
     2341 gtcacgaagc agcggtcata gtacctgcac gttcttcgac cacacggttt gccttcgctc
     2401 gaaaggcgga cggcaagaga gtacgcgccg ggaacatacc tcagcagtgt ctgaaaggcg
     2461 ccgttttcct ctaaatctcc cgactctgtc tcatctatcc agcaaaccag cggaagcagc
     2521 agcgcccggc tggcatccac cgcctcggcg ggcggcaggc cgatttcctc atgccactga
     2581 tgaaggcata cgtaattgtt cttgcccttc aacaccactg agcgcacggg aacgtcgagg
     2641 gttcctgcaa cgagcggcaa atccttgtag aacaactgct cttgcagatt cttcgtgtgc
     2701 gtgctgatta cgactcggtg actgtttttc agggcccacg ttacagaggg gacgaggtac
     2761 gcgagtgatt tccccgtacc ggtacctgcc tcacagagaa gcgtaccgcc ctcgttcagt
     2821 atctcagtga cggctcgcgc catgtctatc tgcgaccgtc tcacctcata cgtgggcatc
     2881 gcccgcgcaa agtcaccgtg agccccgagt gtctccgcaa ttgattctgc gtcaatgtct
     2941 tcaaggctgg tagccggttc cagcctgttg tcatcgaacg ccccgaacgt attgggaagg
     3001 ctcgtcaaag ccccgacctg gagaggcggc gcggagacct ggtcgaatgc ggagcgcgcc
     3061 gccgcggcct gtatatccag aagcagatcc gcgatcgctc cgaacggagg ttccgggcga
     3121 ggaaggctcc gggccagttt tgccagggtg tcgagtcgcg cgggggaaaa ggacctcatg
     3181 gtgttcacga gggccctgaa cacctccacg agtgtctgtg cgtcaccaag cgcacggtgc
     3241 tgttccggat tctcgatgga aaaggcgtca acgagggttt cgagacgatg gttgggcaat
     3301 cgcggcagag caattctcgc cagctccaca gtgtcgaaaa ccgtcggcaa ttctgccgcc
     3361 gggatgctca tccgcagacg gcttacctcg aaatccgcgt tgtgagagat gagaggcgtt
     3421 gcgccgacaa accgccgcag cctgcccatc actccctcga gtgacggttg gccctgcaac
     3481 tcatccgcgg tgattccggt gaggcgttgg atggatacag acggcatttt gtgcggctga
     3541 accagagtct ggaacgagtc tacaatctgc ccgctctgta cgcgcactgc ggctacttcg
     3601 atgatttcgg aggaaaaagg atcgatgcct gtcgtctcaa aatccagcgc gacgaacgac
     3661 tccagaatgc tcatctgggg ggctcttcga aggtgtcagc acgaaggttt tcgatggtgc
     3721 gcaggaatcg cccgtaatgc agagcggaat tggccgggtc gaatgaggga cacgcatatc
     3781 ggaggttgaa atagccggac ggagcgtcgc caaaaccgat tctcaaagaa gctccgcttt
     3841 cggttgcgag gtaggctgtc ggcaggttga actgaggatg aaggtcgacc gcgatggtgg
     3901 gcgcgagcac cttcaccttt tcccgtacgt tgcgtgtggg caggcccatc ctgctgatcg
     3961 cccacgggaa cagaaccgcc ggataaccgg agttcgaaag atccggtagt ttgtcgtcac
     4021 gggaaacgca acacaacgcg agtggaccga accagccggc gagcgtgacg cagatagaaa
     4081 cgccagagtc cagaagctcc ggttcctgcg gcaagaagac aagagtcttg cctcccgcgc
     4141 gaagtgcttc acggatttcc acgggctcgg cggccatact ctgtacgcgc cggtgggcca
     4201 ggaaagcccc gacacgttca agccatgtca tcgttctgag tcctgtccgg ctcggtctct
     4261 ggcaaacggc gttgcactgt ctatggtgtc cccgataggg taacaaccaa gcacgcgaag
     4321 gaacgaggtg agctcgccca gatggtccag agccttgccc acgcggggtt cctccacgtc
     4381 gcctgcaaaa tcgagataaa aaagatactg ccatggggtt ccggggattg gccgggactc
     4441 aattttcagg aggtcgatat cccgcaatgc gaacacacca atgctcttga acagggtccc
     4501 cggcgcatga tggagtgcga atgcgaccga gaccttcttg ttgccgggcc tgtccctgtc
     4561 tgcatccggc tggagagcca cgatcaggaa ccgggtaaag ttctggtggt tgctttcgat
     4621 gcccgaggcg agaatctcca gttcgtaatc catggcagcc tgcgcgcttg atatcgcggc
     4681 agcatcacgc ataccagaag ccttcagctc ctttgccgct cccgcagtgt cgtacacagg
     4741 aaccggcgta actccgggca ggcctcggag gaattcctgg cactgagcaa gcgcctgcgg
     4801 atgagagaac acacgtcgca catccggaag ggcaactcct ttgtttgcga caagatggtg
     4861 cacaatccgc aacttgacct cacccacaat gtgcaggcta tgctcgagaa ggagatcgta
     4921 gttttcgtga acactgcccg caagcgagtt ctctatggga atcacccctc tatcggcctc
     4981 accggtgctg actttgagaa agacgtcccg gaaggttgcg gagggagaca actggatccc
     5041 gtttccgaag tacgcgcggg cggcggcctc gctgaacgcg cccagctcgc cctgaaaggc
     5101 cactacaacc atctcaggat agatccttcc taacattggg gagatggtgc gtacgcacgc
     5161 catcgaggca cacgacaagt acctcgaatg gacgaagctc cattctgacg acgccttcaa
     5221 cagtgatttg agacatctcg ccctgggacg accacactcg cgcagcccgt tcgtcggttg
     5281 gccaggtatg ggcaaactca acgtcggcag gcttgccggc cgtttcggct attacgacgc
     5341 cgactttccc ttcattccgg ttgaggtacg tttttgcaaa gacgccggac ggtttgcgca
     5401 cggccagatt ttcctcatcc cggaagtctg cgtacacgta atacggcgcg actcggctac
     5461 gcaattcggc atggctcttg atgaaggagc aaaattccgg atagtcgctg atatttccat
     5521 cgcccccgtc aatcttcatg tcgagttgta atttgtacgc gaaagctctg ttcgcatccc
     5581 cgtattcaag ggcatctatc tcgcaactgg cgaacaacca ggggagggag aacagaacgg
     5641 cttccgggaa gtcgaacagc ccgcgccacg tccacgaact gtcacaccat tggttcacaa
     5701 aatcgttgaa accctcgccg acaatcactc catcgggatt cgcatctcgc acgattttcc
     5761 gcactcccgg aaggagcgca gcgagaccgt ccacaaatgc cctgtcaggt gctccatgcc
     5821 cgtgggaagg gttgtagcac acgaacatct tctccgcaat ctgatccagt tgcgtgttgc
     5881 caaacccgta gtccttgacg atacgcgtga cggcgtcgag aaaatgtgga cgatattcct
     5941 cgcagaagca gacctcgtgg tgtaacatgg atcggttgcg ggatggagcg tcaaaattgt
     6001 tgcgtgccca gtccccggcg taatacgggt gaccagcgcg tgttatggct tcccagcgat
     6061 atccgtattc cctgtgttcc ggcatctgcg tgtttgccag tgtgccgttg aagaacggaa
     6121 tcacctgtac tccaagctgc cggcattcct caagagcggc tttcaatgcc gcttcgccgc
     6181 cccaggagtc gtttgggacg taacggtaca tgtagttgtt atcgtgtcct cctgtctgcc
     6241 acccgaagac catcagatac ctgcatccag ctgccagagc ggacttcgcc atgcgcggaa
     6301 ggtcagcgta ggagttcacc tcgagtccgt cctgatgttt catgaagtag aaatgccatc
     6361 caatggactc ggcaaattgc gcgggccttt cgggctttcc tatccatgtg tccagccagc
     6421 cccggtgttc atccgcgacg gtgtgccagt ctccttcatg caggcggatc cgtacgggag
     6481 gcgtcgtgaa ggtctctccg ggtgctaccc ggtgcgggtg cacagtgtac agatcgagag
     6541 agcgccgaac gggatcgaaa tgcttctcga ccactttgtg caggcagaaa tccattatcg
     6601 agaaatcttt gtgccgccct tccagggcta ttccggagct gtcgttgcca tagtcgcacc
     6661 atgccgtggt cagccagtgc ggataccgcc ccacgtattt ctgggccatc tcgttccacg
     6721 agtggtggtc gccgccgagc cctctaccga atacgtcgtc aattctgcgg aaatggaaat
     6781 gaggccacgt aatgcacgcc ttttcaacgg gcgcaagacc tctcacatac gggaacatga
     6841 tctcttcaac cggtgcgggt ccatcgttga cgatctgcag gcggcagacg agagcgtctc
     6901 cgtccagttc cagaaacgcc tgcatgctga tcccgttccg ggcgaacgtc agaacgcagc
     6961 tcgaccctcc ggggacaacc gccggcacgt cactatctac gtgttcgcat tcacgttcac
     7021 catccggcac cattaaccgg aaaaggaggc tccgatccgg cgcgcacacc cattcccggt
     7081 ccagacgcaa gtccttcagg gaccggaatc ccccggtttc gtcgccaagt tccacccgga
     7141 taaggtcatt ttggaggatc atgtgtgagc ttccacgtgg ttgcgcaggt tgtctcccgc
     7201 cggtaccttc gtaggttgaa acccgcctct gacgccattc caaaaggagc gcgcgatgat
     7261 caaaaccagc acgtacccct gcaaaatgat agccaaagac attcccggtg ccgagggccc
     7321 ttgtgtcaac atgcgcggtg agttcttcat ggtggcgccc aacctcggcc aggtcttccg
     7381 cgtgaatgaa tctggcgaga cttcgatatt tgcggagaca gggggcattc ccgcaggtct
     7441 gcagtgcgac aggagcaaca gactctggct tgcggacatg aaacgcggca tcctttcaat
     7501 atcccccgag ggtatcgtac gtgtggaaac gccggagttc gacggcaaac cgatgcgagg
     7561 ctgcaacgat tgcgcgtttg attccagggg caacctgtat gtcacggctc cggcggggag
     7621 tggcaaagat gccccgaacg gagaggtctt ttgcaggaag aacaacggag aactcgttct
     7681 tctggacggc ggctttcaat tctgtaacgg tattgccgtc aacgcggacg accgcatcct
     7741 gatcgtggcc gaaactctta cgaggtggct gtgggcgtac gatatccttg cccctggaaa
     7801 ggttgcaaac aagcggaagt gggctcaact cgtcgatgtt gaaggcggtc ttggtcccga
     7861 tggtctggat ttcgattcag ccggtaattt gctcgcggcg tactggggcg gcggttcctt
     7921 ggacgtgttc aaccccgcgg gtgagctcat cgagcgcatc atgcttccgt tcaaggcacc
     7981 ttcgaacctg cacttcggtg gaaggggcag ccggaccatc tacatcacag aacacgattc
     8041 aaacggactc tggaagttgg agtgggagcg cgatggacag ccgcagttct gcgaacgctg
     8101 agaggtatcg aacgtgacac cgagagaacg gctccgagct gcctgtacag gctctgctgt
     8161 ggattgcgca ccggtagtca tcccgtatca tgccatcttc ctgcgcgatc actgggagga
     8221 ggtaaccaca gaaccctggt ggaagacaag cgacggcaat atctcgtcgt cagttcaggt
     8281 ggaacgcgac ttcctcgaat cgacgggtat ggattggaca ggtgttggta tcggagcttc
     8341 acggcagttc agggaaaccc atgcagcatc tgttcgtgac ggcgtgccct acctcacaaa
     8401 cacggtaacg ggagaagaaa cggaattacg gcactggcct cgaagcggcg accactactc
     8461 cgtgaactgt ccatcagtca ccaacctgga ggacatcgaa cgggtggtgc aagtgagatc
     8521 cgcacgcgaa atcctcgacg cgggttttct cgatggcgca cgtgcggtgg tcgaatccgt
     8581 tggcgcttcc cacgcagtct acgccgcagt gaaccatccc tcgtggacgt atacctgctt
     8641 cgggttcgag aacatgatga ccaacatggt cgagaaccct gcattggtgc acgctctctc
     8701 cgagcggatg tgtgagaagg ccatcgagga aatcaaggcc ttcgccgcaa tcggcgtaga
     8761 ttttgtatgg gttgagttgt gccttgttgg acgcgattct atctctcttt ccgcttttcg
     8821 cgaattcgtt accccttaca gccgtccgtt ggtggacgtc gccagagaga acggcatgat
     8881 caccgtgttc tacgtatgtg gcgacgtcag cgaccggttg gaagaccttg tgtccgtctc
     8941 tccaatgtgt ctttccttcg aggagagcaa gaaggggttc tgcgtcgatc tggacacgat
     9001 ttccagggtt gtcggcgact ctgtatgtct ttttggcaat ctcgacgctg tcgacgtgtt
     9061 ggagaatgga tcggacgcgc tccttgcacg cgaaatcgag cggctgctgg aaataggcaa
     9121 tcgtaccggc agattcgtga tgtcgctcgg gagtccggtg actcccgaca ccccggtcgc
     9181 tcgcgtgagg cgatttggcg aactgactcg ccagttcagt tcgcgatgat cctgcgaatg
     9241 gagcggttga caacgtgtgc ggaagaacgc ctggcggaag gggtgtgcgg tgagtgacgc
     9301 gaagcgcctg tatctcctcg acggaacggc actcgtatac cgtgcctatt ttgcgttcat
     9361 ccgcaatcca ctcatcaact cgaagggcga gaacaccagc gccccgttcg gattcgtgtc
     9421 cgctcttctg aaaatcgtcc gcgaggaggc ccctgactat ctcgcggtag ttttcgattc
     9481 cgctcagcct acgttccggc atgaacgttt ccccgcgtac aaagcgactc gcgaacggat
     9541 gcccgaagaa cttgtcgccc agcttcccag aatcgaccag atcgtcgaag cgttcggagt
     9601 gccgattgta cgtctggacg gattcgaagc ggacgatctc atcggaacgc ttgcagttca
     9661 ggggcaaaac gccgggctgg acgtgacgat tgttaccggt gacaaagatc tgatgcagct
     9721 tgtgaacgac cgtgtcagga tacttgcgcc agggaaaacg aaaaacgaat gggagcggat
     9781 cgggcccgag gaggtcgtgg caagatgggg tgtctccccg gaacaaattc gcgatctact
     9841 cggtctgatg ggcgattcat ctgataacat tcccggcgtt cccaaggtag gggagaaaac
     9901 cgcggtcgaa ttgctcaaaa aatacgggtc gctggatgcg gtgcttgaag ccgcgccgga
     9961 aattcaaaaa cccatggtac gccagaatct gatggacttc gcgttgcagg cgcgcctttc
    10021 caaagagctt gccaccatag cggtcgatgc tccagtcact ctcgacctgg aggcattcac
    10081 atgggtagca cccgatccgg ctcgcgtcgt accggtgttt cgcgaactgg aattcaatca
    10141 gttgatcgaa cagctcggtg ttcaggctcc cggtcctgcg aataagctcc ccctcgaatt
    10201 ccgaaccgtg acggcggaca tgcttccggg cctttgcgag caaatacggg cggccggagc
    10261 cttctccttc gacaccgaga caacatctac tgatcctcgg tccgccagtc tggtcggcat
    10321 ttcgcttgcc tgcgacgggc tgaccgggta ctacctgccc attggccacc gaaacagaga
    10381 accggacggc ctggccttca cgcggtccgc cgaatcaccg gtcaatcttc cgctgacgac
    10441 ggtgaaggag catctgcagc cgttgttcgc cgatacaatg ctcctcaaga tcgggcagaa
    10501 taccaaattc gatctcgcca ttctgaaccg cgccggtttt tcagtcgacg ccccgttgtt
    10561 cgatacgatg atcgcgtcct acatactgga cccgtccgca cggcatggcc tcgatgcaat
    10621 ggcggaagag catctcgggc accggatgat tcccatcaca gacctgatcg ggaagggaaa
    10681 ggacgagatc accttcgacc ttacacctgt tgataaggcg acggagtact ccggtgaaga
    10741 tgcagtaatc acctatcgtc tgggcaggct ttttgaggag cgcctgaaaa caaacggcct
    10801 tgacgagctt ttccgcaccc tggaaatgcc gcttgtgcct gtccttctcc agatggaaga
    10861 aacgggcata cgcgtggacg cagaagcgtt gcgcgacctc tcgcgggaga tttctcaaca
    10921 attgcatgag ctggaggagc aggtttacga cgcggcgggc gagaagttca acatcaattc
    10981 tactcagcaa cttgcgcata tcctttttga cgtgatcgga cttcccgcac ggaggaagac
    11041 gaaaaccggc tactcgactg atgtggatgt tctcgaagcg ctggcaccgc tccacccgtt
    11101 gcccaagctc gtcctccagt accggtcgct tgccaagctc ctttccacgt atgttgatgc
    11161 tctaccaaag cttgtcaaca aaaagacggg acggatacac acgtcgttca accagacggt
    11221 gacggcgacc ggtcgactgt cttctaccga ccccaacctg cagaacatcc cggtaagaac
    11281 cgaactcgga ggacaggtga ggcgagcatt cgtacccgaa aagggatggc tgatgctctc
    11341 cgccgattac tcgcagatcg aattgcggat catggcgcat ctctccgaag acaccacact
    11401 gatcaaggcg ttccagaatg acgaagacat ccacacgctc acggcttccc tcgtattcgg
    11461 ggtgccaccg gctcaggtga accgcgaatt gcgcgcacag gcgaaaacga tcaacttcgg
    11521 agtaatctac gggcagaccc cgtttggcct gtcacagcag cttgggattc ctcagcgtga
    11581 agccgcgctg tttatcgaga attacttcaa gacatatccc ggcgtcaaaa agtacgggga
    11641 agtgaccatc gagcgagcga gaaaagacgg gttcgtctca acaatgctcg ggcgcaggcg
    11701 gttcgtcccc gatattgacc accaggacgt taaccggagg cggttcgccg aacgaaccgc
    11761 catcaatacc cccatccagg gaaccgctgc cgacatgatc aagctggcga tgattcgcat
    11821 tcatgaaagg atcgccgccg agaagctccg ggcgcgcatg cttcttcagg tgcacgacga
    11881 actgctgttt gaggtcccgc ccgacgagtg cgacgcgctc aaagatctcg ttcgtgcgga
    11941 aatgtcgggt gctctggaac ttcgtgtgcc agtacgcgtg gatataggtg tgggcgagaa
    12001 ctggttggtc gctcattgac ccggaacgaa cgcaacgcgg gagaactacc atgccatcgc
    12061 gcaactccgt cctgtcgggt gtttttgtgc tgggagttct cctcctcgcc ggatgtgacg
    12121 agatcactcg gagcggagat ccgggtggag cgacaggcgc ggcagacccc aggcttgcca
    12181 aagaagctgt tccgcccgca cttgtcgacg ccaacatgcg cttcgcgttc gggatctacc
    12241 agcgtctggt agagacaccc cccgcagaaa acctcttcat atcgcctgcg agcatcagtc
    12301 tcgctctcgc aatggcctat aacggcgcgg caggaaacac gaaggaggca atggcgcgga
    12361 ccctggggct gggggaaatg acattggagg aagccaacca ggcgaacctc gtgctcctct
    12421 caaacctcgt gtacgcggac gaaactgcga aactcgcaat tgccaattct gtctgggcga
    12481 accagggcta cggtttctac gagagtttcc ttgaccgcaa tcgccgctat ttcggagcag
    12541 aggttgccac cctcgatttt gccagacctt cggcccttgc cacgatcaac gggtgggtgg
    12601 agaggaacac gttcgggaag attccgagta tcctggacga gataccggat ttcgccatta
    12661 tgtacctgat caacgccatc tatttcaaag gtgaatggac gaacaaattc gacgttgcgg
    12721 aaacgcggat gcggccgttc accctgagtt ccggttccgc aaagaacgtt cccatgatga
    12781 cgcggcatgg aaggtatgcc tacctccaga acgatctgtt ccagggagtc gcattgccgt
    12841 acggaaacaa acgcttcagc atgtacgtgc tgcttccaaa agaagacgta ggcctcgcag
    12901 ctttgaacag agctctcacg gtttccaact ggacgacctg ggtcgccgca ttcgatacca
    12961 tggaggggac gatttccctg cctcggttcg aggcacggta tgaggagacg ctcaattccg
    13021 ttctctcggc aatgggaatg ggcgtcgcgt ttgatcctta cggagcggat ttcagtgcca
    13081 tgctgtccgt cagcccttcc cggaacgtgt tcatcaacga agtcaaacac aaaacctacc
    13141 tcaaagtgga cgaggaaggt tctgaagctg cggccgctac ctccgtcgga ttcggtgtaa
    13201 cgtccgttcc cgtcacgttc aacatggtcg tcaatcgccc gtttttcctc gtgatccgcg
    13261 agaacaaatc ggggacgatc cttttcatgg ggacaatcgt cgacccgtct tcatgaagcg
    13321 cgggaatgca tcacgcggat ccaggtcttg cctctgtacg ctgcatcaca gcatagccaa
    13381 cgccgaccac gtacctttgt atcgacagga ggggctttgc ccctcacgct tatgaatcct
    13441 acaaaggagc gagttgaatg gcgttgcaga agacaattcg aacggccttt ttgtcattgg
    13501 gtgtgctggg tgccgcgatg atgtcgggtt gtatcctgag taacgaagac gaagcaggaa
    13561 cggttaaaga ccacgcgggt ccaaatacgc ccgttacggt ggagaatgcg cagaccgtct
    13621 tcaacatggt cttcgggacg atcaaaggcg caatggaaga gcctctcagc aagccgtccg
    13681 cggccgccag cgagacgcgt acgatcaacg ggagcgcgag cggcaaaatc gttgtgaaaa
    13741 ccacgccgac tgccaacggc tataccgagg agctggagat aaccgacttc tcaaacgatg
    13801 gtgagctgtt cattggcgga aagttgacca acgacatgac gacaaccgag cacggtgaaa
    13861 ctggcaacat cactggggat atcgccgtgg ccggtttttt caaagggaaa gtcggagtag
    13921 acgttatgat ggagaatttc ggcgactact tcatggttca agggatggtt acggtcggcg
    13981 gagaggaagt ctccgtgttc ttccatccta cctacacgaa agtggcccgc tgaaacacgt
    14041 cgttccctgg ggaacatcaa gacgccggcg gcttctgatc gccggcgtct tttcgttccc
    14101 cggaaggagc aataacccct gcctcgtgtc tgccggactt aagcgttggc gcaagggccg
    14161 ccactctgtc gatcatgtcc tctttctcgt aaatatcctg cgaatactga tgacggcgca
    14221 gttcagcata cgttgccatc tgtcccgcgc tccatacgcc ggcggcgtac cccgttacga
    14281 ctgccagaag gaaaaggatt ccgaaggaga cgaatgctcc cccgacggcg acaccggggc
    14341 cgacaacgat gctccagacg tcgaactgcg ggacgactcg cgaaagcacc caatccagcc
    14401 ctggaacaaa ttgtgacgcg acgtacgcca ctgccgcgag tctgccctgg gttgtcaatc
    14461 cgaccaagac tatcgccagt cctgtgcagg tgaacaggag gcccgtagcg gcaagccacg
    14521 aggcgatggt gatacagaaa acgattccct cgtatacgag gaaccgccac cattgtgacc
    14581 acagcaacgc gcccacgtta accagcgttc ccagccagtc atcatccagc gtattggcga
    14641 tcgcgacgcc gacggtgagg gtgccagcaa taagcgccag gcagaccagc aggaggaata
    14701 cgagaggcaa cgccagcaga aacccgaaac ccgcgaaaat ctcccccgcc acgggtattc
    14761 tccccggcag tccaagagcc agagcgctca gcagtgttcc ggtggcaacc agtatgagaa
    14821 gcgcgattgc agtcacgagc gcgtggctgt tcgctcgacc ggaatgccac gcttcatgcg
    14881 ccgaaaacga aacgtcgcct ttcgcgtggc ggaaaagggt ttttacctgc gcgccggtag
    14941 aatacagcag aacaagccct gcaattgcct tccccgtcgt ctgcacgaat gttcccaccg
    15001 ggttcagccg cagatccgcc aaccagggaa acaggcgtaa cttgcgccac gcaaacagga
    15061 gaggtatgga cgggtcggcg agcaccgcga gatacgtgaa aacacaaaga atcaccgaac
    15121 agaggaggac agcaatcgtg cctgttcgga tgactgtgcc gttcagtgcc attctcggtg
    15181 ccgagagtac ggcacgatag ccgtgggccc catttgcacg tgccaatcac gccc
//
LOCUS       DAOSOO010000061        15175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_3938_length_15175_cov_5.692659, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000061 DAOSOO010000000
VERSION     DAOSOO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 15175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15175
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3938_length_15175_cov_5.692659"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLT86_06130"
     CDS             <1..587
                     /locus_tag="PLT86_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36223.1"
                     /translation="NGKKVLVGGSIGSFDALLRSDDLGATWRLQPIRTALGDTSRSGQ
                     GVTDVAWQNGTIWVTTGLGLARSTDDGHTWAYILRYPQTELLGSSGVAYPGGDAAGIA
                     TYAFPSPCAPLRGTPARIVFALAATADVTIEVYDAGGARVRTLRQTSLPPGNQTVEWD
                     GRSDGGRVVANGVYLYVITTSTGHTARGKMMVFN"
     gene            584..1516
                     /locus_tag="PLT86_06135"
     CDS             584..1516
                     /locus_tag="PLT86_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36224.1"
                     /translation="MKTARFLLAFAVLAFCSGTTRASGGYANPLYRYGIGSNVLGVGG
                     AVVSLAYDPASILVNPAGAAFAGRGRIAASHSALAFDRTINFIGFSRQIDPQAGFSVS
                     WVNSGVSDLIGYDLSGNSTGPLDNSSNTVAATFASKFGPIAAGVSAKWHRVSLNRQQS
                     AGWSFNVGALARPLPGLSIGVSARDLLGTTVWTTETDVGQLRSEDTFPVTLSGGVSYA
                     VARLGTTLAADFEHVGSEGDYLHVGTSVVVSQYLRVRAGYRWVGIAESCRKAAFAGGI
                     GLSTTFGDNRVHFDYSVLNEALGAAHSLGMAFEL"
     gene            1561..2463
                     /locus_tag="PLT86_06140"
     CDS             1561..2463
                     /locus_tag="PLT86_06140"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT36225.1"
                     /translation="MLAPLTQAARAIEVGSTGYKFLQMNPDASAMALGRAFVTERGGA
                     TALAGNPAGLAGVGTVSAVVSHTEWLSTIRHEFAGAAIRRFGGVIAASFRTQSSGDIP
                     LRTADTGGGLIGLPTPAPLGTYGVYDAALTIGYARATTLADVGGSVTYAYEKIFTAES
                     SAMCASVGMQRRAGDLILGAAARNFGFSSRMKYERTPLPWDIQAGAAYDRHIRDVPFR
                     LVGDFRYAPDWYATFHLGTQATFGNVLVARAGYENGLTRNLDTAGFSGGLGFLHRGFR
                     VDYAYQPQRTGLGSRHVFAVAIGG"
     gene            2538..3503
                     /locus_tag="PLT86_06145"
     CDS             2538..3503
                     /locus_tag="PLT86_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36226.1"
                     /translation="MATKGLQVGFIGAGGMAQSHMKGVARLSDVKIAAICDVDEQRAQ
                     SVAKEYHAAAYTDFRAMLRKKLDAVYIVLPPFAHGEVEKAVIKKGLPFLVEKPIALSA
                     KLGEKILDAVEKKNLITCVGYQLRYMSSIQQAKAFLDKKTLGMIVGYYWSEMIRGAWW
                     PYMEKSGGQVVEQATHIVDLMRDLGGEIVEVDARMEQRVHGKNDGITIPDTYSVRFGF
                     ANGAIGTLTTCCLVGEWNIGLDIMVDRARLHWKIDELTVAPDSVTLPAVESFKAGDID
                     ATFLNAVKTGDRSAIRSDYRDALKTLKVTLAATESASKKKTIVLK"
     gene            3542..4873
                     /locus_tag="PLT86_06150"
     CDS             3542..4873
                     /locus_tag="PLT86_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36227.1"
                     /translation="MAEMLFTFASYATRIEGSLTPLKGMKRLADTLHPYGVKITWLVN
                     HDSAEALKSDLTEWHDRFGDDIAVMVPHFKGGAPELVAQARAARQRMQKILPWTRADI
                     AGNHVRSRESLESLEAAGFIGLWGFCWEQIEVDDITDRGCPWGFYYLDPAFRTAPNTR
                     SKGLIGMEWTARDLLKAYHSGNPCIYSTDVNDVARAGMCLWRDIEYLKGMFDNYYRNL
                     RYNDLVLYQVHQEAHEMMPEFRCYSEEDCREAEEMLDELADYVVGHAHIRTATLSEAA
                     DVYRERNDFTEPSYMLWEDTPAPPYNLDYSRGTPRGPWPKTFLFYDSECQMMFVDGKF
                     EPVCLRNYADRETTARRYFEEVDIPRVRLRSHTRSPYGMELHLTVEAPKAMPFGITIW
                     EDFGPYLLAESVSEPTGTDLMASRILSQELLLLRYNVPEGKTELRVRFNRK"
     gene            complement(4965..5498)
                     /locus_tag="PLT86_06155"
     CDS             complement(4965..5498)
                     /locus_tag="PLT86_06155"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT36228.1"
                     /translation="MAKQDIQFTAYLSQSLEVLSRGGALLVSTGKNGRPNVMTIGWGT
                     VGWIWGKPIFCVLVRPSRYTHECLEATPEFTVNIPPRSLAGAVAYCGKVSGRDEDKFA
                     AQNLTATPGRRVAPPIVEECVIHYECRVVEKNNVIQETLSAEIDRSAYRSGDYHTLYF
                     GEIVAVYADADALAKLA"
     gene            complement(5518..6816)
                     /locus_tag="PLT86_06160"
     CDS             complement(5518..6816)
                     /locus_tag="PLT86_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOT36229.1"
                     /translation="MHFLEKLIESRQAQVGIVGLGYVGLPLAVEFAGAGLRVIGFDMQ
                     PAKVDSVNNGVSYIGDVPDERLVPLTKSGRLRATNNLSDLGQCDAICICVPTPLDKNK
                     TPDISYVASTADAIAQNLRDGQLIVLESTTYPGTTEEIILPRLTATGRIVGKDFYLAF
                     SPERIDPGRKDFTTANTPKVVGGVTPQCTELAAALYSTFNPRVHRVSSPRVAEMEKLL
                     ENIFRLVNVSMINEMALLADRLGVDIWEVIEAASTKPYGFMPFYPGPGLGGHCIPIDP
                     FYLSWKAKEVNFSPRFIELAGEINDMMPHYVTTKVIVALNRQKKCVNGSKILVLGVSY
                     KKDVEDVREAPAVRIIRELWRKFALVTYCDPHVRSLSIDGKPVERVELTDEALREADC
                     VLILTDHTAFDFDRVAEKSRLIVDTRNAITRRDLSHVYHL"
     gene            complement(6913..8016)
                     /locus_tag="PLT86_06165"
     CDS             complement(6913..8016)
                     /locus_tag="PLT86_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36230.1"
                     /translation="MEIPSSEQAREAIKLAVGRATERYVAQIAPEGETIKFPELLGKA
                     LPTMALRVLRSVEDSFWRKELERRKNDQRFDYADPALQTAVATWDAALRKCMVLSRKE
                     LHQLINRALSLQMESLVSPIDSLKANYFVRQEKVAAKSAVVLASHLGLEERYVRALSL
                     MAQDDEKRLLSADTVQRVFRDVDAKEFQGSKDRAAVSVLSNALLVLGIRAEDELGEAP
                     VDLALAVMVLRGTPEGTAKVAELCEGRALVDMLDVDSLFMPEQAAVTEDESHEEAQAF
                     LDDLGIDQDAVEAGGAELETGGVRFVLTEEEKRAYISRAVGRHTHLIEPIMKATEAAL
                     TWEEVDRAINDLVPDDDRDEDLSARFRERLGRR"
     gene            complement(8066..8554)
                     /locus_tag="PLT86_06170"
     CDS             complement(8066..8554)
                     /locus_tag="PLT86_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOT36231.1"
                     /translation="MNKQGGSLSAEQMQTIESVLAGYVKDAGIRTVLLVDPDGHLVCQ
                     SGAMDTIDPVSFGALVSANFASTQQIAGQMGEKEFNAFYIQGKEKDLYVSAVEDKAIL
                     VSIFARGTTVGMVKIFAEKTVKEISANLVPGEGGDQKGLSVGGGFADSALAELDKVLG
                     GI"
     gene            complement(8606..9199)
                     /locus_tag="PLT86_06175"
     CDS             complement(8606..9199)
                     /locus_tag="PLT86_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT36232.1"
                     /translation="MATINYGYREINCKIVYYGPGMSGKTTNLETIHKKVPPTNRGKL
                     TSLATKTDRTLFFDLLPLDLGTIKGFKVKFQLYTVPGQVYYNATRKLVLRGADGVVFV
                     ADSQKDRYDDNVESLENLRENLKEHGMDLDAMPFVIGWNKRDLPDVMSVEELNAKMNP
                     RGVPNFEMEALTGRGVFEALRAVTRMVLEKLEKEGTR"
     gene            complement(9231..9623)
                     /locus_tag="PLT86_06180"
     CDS             complement(9231..9623)
                     /locus_tag="PLT86_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36233.1"
                     /translation="MSEQVLEIVTPLAERPDVVRVVVSDREGLLIGALKGKAASGSMR
                     DEETTDEQWSAFVAQFVTDAGAQLKNITLHHPTDMVVHGTRDSLVITWLHVGWLITRV
                     SPVADWGDLFATVRAVQQQFDKVTGESA"
     gene            complement(9616..10317)
                     /locus_tag="PLT86_06185"
     CDS             complement(9616..10317)
                     /locus_tag="PLT86_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36234.1"
                     /translation="MPAALFDRLNDLQAAIEAENWADFDAVLKSWSAEEPENAWVSAG
                     LTLAKVLRGDEVPDTEASLQAINGRLAGMTGLLAAVRAGDKVEARKRALAELAAGNAD
                     QSLVETLKWADEAASDATRYLFATPGLVAGCVMAGGQPVSMTARGSRMSADSLGTKSE
                     AIWELAIKMNQFIDLGDLTGIRVFGEDGGWTLGAAYADDLRLVAGLVETASNMDELAA
                     RTRLLVAALEGPGNE"
     gene            complement(10317..12137)
                     /locus_tag="PLT86_06190"
     CDS             complement(10317..12137)
                     /locus_tag="PLT86_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT36235.1"
                     /translation="MDAPQKKSNPPAPRVGPGRADGVRLPEFLQKQVDAIDETAILNE
                     LIAAISPELLDLEPRTETVPAPAAAPAPPPAPAPREKQQPSPPREQKEEVEEPTAKST
                     TPLKGLQIRPEEVEEAISSILGPTEPQAKVEKEKPAEKKEAPPPPKAEPAPTPPPEPA
                     AAKAGPKVVAPPIKPPAKQEFDSVDDALSALWGKGEEEASTAVEPVAEAPAAAPTAAP
                     PPPPVSEPAPPAPSPPAVEKAPTEPAKVPAVEGGEIPPALVPGLARMAATHYLNLLGT
                     VLPSGAAPPPAAEKAPPEPSAAPPPAETPPTEEKPFAGLQEADVTADLLADFIVEERE
                     GAGEEQKEAEPASLEEVVSSAILEPPVVEEKAEEPVPVGQPEATAAGEEGEEGKPAPW
                     TGPIADIGGDDVIAEMAKEHRRKKLIGWVIVAFIAVAFASFFFGPKLKELISGLSTGT
                     TGSPAAVTPAPTPQPAPAADRVAQPAPPPAPAPAAEATTPAPPPAVAAPAPAPAPPAP
                     RPSGGAPQLPTSPGGRFTVHVESLPTLGEANAAKSKWQRRGYEALVWPFTKNDGSQWY
                     RVGVGRFATKADGQTAKSALESAFPEIDWSQVTQIPEGAN"
     gene            complement(12548..13861)
                     /locus_tag="PLT86_06195"
     CDS             complement(12548..13861)
                     /locus_tag="PLT86_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT36236.1"
                     /translation="MTPHLIWRKETGYQLNGIVSGMVDGQVRLYLSGIRTVSGRRESA
                     IFCLSSEGETVWQTQVADAAEDRLACVFPNLIDDPFLGLCLFYSFGSSDNARPRPGYA
                     RLVRAESGDVVWEVRSRSHHAGNGSCIAADLDGDGRPEIAWWDSANVFCSDLRTGDLR
                     WEYSDRVQICHGRPALAHLRGIPAIIVGTEYSNQDGTSSVLALDGSGGVLWRKDGFPE
                     DLGSTPVILADVDHDGRDEFVIAGLDLVHRDNRETSSLWCFRDDGELAYRVPCGCGGL
                     AVARVRGELLAAGITNTRDGGNNGAQEVRLFSLATGVLRHSDPLPRAYLDAQNPVAAD
                     LDGDGLPDFLLTTGNPSGYGNRPAIRPYADVYVVRADGATLWTETFPDYIHQPFVSDV
                     DGDGRNEMLLPCGDGSLYCIRTPGIATGSVWPCTGRSYSRWYGAT"
     gene            14043..>15175
                     /locus_tag="PLT86_06200"
     CDS             14043..>15175
                     /locus_tag="PLT86_06200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT36237.1"
                     /translation="MADTLAIRGGTPAVPPGLKFRMWPEITKEDEELVLASLHQDSHA
                     FGPHADMLQKEFAAWNGNTFCMATNSGTAALHMCVAACGIGPGDEVITTALSWTSSAT
                     CIIHHNAIPIFVDVDWRSMHMDPSKIEAAITPYTRAILVVHYWGVACDMDAIMSIARR
                     HNLAVIEDACQAHGATFKGRKVGTIGDVAAFSLNQNKNLCGGEGGLFVTNDPGLFERG
                     KMVTSFGDMRPPDSGRDYHAYGLGWMYRTSDLPAAFALSQLRKLDRTNKWARDNWDML
                     DARLSGTPNLVRSFTSEDRPTNGYAYVLRVEPAYAQRRGVPSRKITEGIVAALKAEGV
                     NFSIANWLLPAHAVFQAKNAYGKGEPWTSAFARKDVSYDLSQYP"
BASE COUNT         2914 a         4559 c         4567 g         3135 t
ORIGIN      
        1 tcaacgggaa aaaagtgctc gtcggcggct ccatcgggtc gtttgacgca ttgctgcgga
       61 gcgatgatct gggcgcgacg tggcgtcttc agccgatccg aacggcgctt ggagatacgt
      121 cccgttcggg gcagggggtg acggacgtcg cctggcagaa cggaacgatc tgggtcacca
      181 cggggctcgg tcttgcacgt tccactgatg acggtcacac gtgggcgtac atcctgcgtt
      241 atccgcagac ggaactgctc ggaagctcgg gcgtagccta tccggggggt gatgccgccg
      301 gaattgcaac ctacgcgttc ccgtccccgt gcgcacccct tcgagggaca ccggcgcgga
      361 tcgtgtttgc gcttgccgcc acggcggacg tcacgatcga ggtgtacgac gcgggaggcg
      421 cgcgggttcg cacgttgcgc cagacatcgc ttccacccgg caatcagacg gtcgaatggg
      481 atggccgttc tgacggggga cgcgtggtgg cgaacggtgt ctacctctac gtgatcacca
      541 cctcaaccgg ccacaccgca cggggaaaga tgatggtgtt caattgaaga cggcccgatt
      601 cctgcttgcc ttcgccgtgc tggccttttg cagtggaacg acgcgcgcat cgggcgggta
      661 cgccaacccg ctctaccggt atggcattgg ttccaacgtg ctgggggtgg gcggcgccgt
      721 ggtcagcctt gcgtatgacc cggccagcat tctcgtaaat ccagcgggag ccgcgtttgc
      781 ggggagaggc cgaatcgcgg catcccattc cgcgctggcc tttgatcgga caatcaattt
      841 catcggtttt tcgcggcaga tcgacccgca ggccggattc agcgtgagct gggtgaacag
      901 cggggtgagc gatcttatcg ggtatgacct gagcggcaac tccacgggtc cgcttgataa
      961 cagcagcaat acggtcgcgg ccacctttgc gagcaaattc ggccccattg ccgcaggtgt
     1021 ttctgcgaaa tggcaccggg tgagtctcaa caggcagcaa tcggcgggat ggtcgttcaa
     1081 cgtgggagct ctcgcccggc cgcttcccgg cctctccatc ggggtttccg cccgcgatct
     1141 tctcggcacc acggtgtgga cgacggaaac ggacgtggga cagctcagga gcgaggatac
     1201 gttcccggtt acgctgtcag ggggcgtgtc gtacgcggtt gcacggctgg gcaccacgct
     1261 ggcggcagat ttcgagcatg tgggaagcga aggcgattat cttcacgttg gcacctccgt
     1321 cgtcgtcagc cagtatctgc gggtgcgggc ggggtaccgg tgggtcggca tcgccgaatc
     1381 ctgcagaaag gccgctttcg ccggtgggat agggttatct accacgttcg gggataacag
     1441 agtgcatttt gattactcgg tgctcaacga agctctcggg gcggcgcatt ctctggggat
     1501 ggcattcgag ctttgaacaa tcgatttcat ggactcacgg tgttggcggc ggcagggtgt
     1561 atgctcgcgc cgttgacaca agccgctcgg gcaatcgagg tcggcagtac tggctacaag
     1621 ttcctccaga tgaacccgga tgcctcagcg atggctctgg gaagggcatt cgtcacggaa
     1681 cgcggcgggg cgaccgcact tgcgggcaac ccggcaggat tggccggtgt tggaacggtt
     1741 tccgccgtcg tatctcatac ggaatggcta tccacaattc gtcacgagtt cgccggcgcg
     1801 gccatacggc gatttggcgg tgtcatcgcc gcctcattcc ggactcagtc ctcgggagac
     1861 atcccgcttc gcactgcgga cacgggcggt ggtttgatcg gccttccgac ccccgcaccg
     1921 cttggcacgt acggcgtgta tgacgccgcg ctgacgatcg gctacgcccg cgcgacgact
     1981 ctggcagatg taggcggaag tgtcacttac gcgtatgaaa agatcttcac ggcggagtct
     2041 tccgccatgt gcgcgagcgt tggtatgcag cggcgcgccg gggacctgat ccttggcgcg
     2101 gctgcccgca atttcggttt ctcatcaaga atgaaatacg agagaactcc gcttccgtgg
     2161 gatatccagg caggcgcggc gtacgaccga cacatccgcg atgtcccgtt tcgcctggtt
     2221 ggggatttcc gctatgcgcc ggactggtac gcgacctttc atctgggaac gcaggcaacc
     2281 ttcggaaacg tacttgttgc ccgggcgggg tacgagaacg gtctgacgcg gaatctcgat
     2341 actgcgggat tttccggcgg actcggattt ctgcaccgcg gatttcgtgt ggattatgcg
     2401 tatcagccgc aacgaaccgg gctcggcagt cgacacgtgt ttgcagtagc gatcggcggg
     2461 taactcgaag acgaacggag cttgtggctt ccgggacaga tccagatttc atacccagac
     2521 tccaaggaga ccatcgcatg gcaacaaagg ggcttcaggt agggttcatc ggcgcgggcg
     2581 gaatggcgca gagccatatg aaaggcgtcg ccaggctcag tgatgtgaag atcgccgcga
     2641 tatgcgatgt agacgagcag cgtgcacaat cggtggccaa ggaataccac gcggcggcgt
     2701 acacggattt ccgggcgatg ctgcgcaaga aactcgacgc cgtgtatatc gtgctgccgc
     2761 cgttcgccca cggagaagtg gaaaaggccg tgatcaagaa gggccttccg ttccttgtgg
     2821 agaaaccgat tgcgctgtcc gcgaaactcg gcgagaagat cctcgacgca gtggaaaaga
     2881 agaacctgat tacctgtgtc gggtatcagc ttcggtacat gtcgagcatc cagcaggcca
     2941 aggcgttcct tgacaagaaa acgctcggga tgatcgtcgg gtactattgg agcgaaatga
     3001 ttcgtggcgc gtggtggccc tacatggaga aatcgggagg gcaggtcgtg gagcaggcga
     3061 cccacatcgt tgacctcatg cgcgacctcg gcggagaaat tgttgaggta gacgcccgca
     3121 tggagcaacg cgtgcacggc aagaacgatg gcatcacgat tccggacacg tacagcgtcc
     3181 gatttggatt cgcaaacggg gcgattggca cgctgaccac ctgttgcctg gtcggcgaat
     3241 ggaacatcgg gcttgatatc atggtggacc gcgcccgatt gcactggaag attgatgaac
     3301 tcacggttgc gccggattcc gtcacgcttc ctgccgttga gtccttcaaa gccggtgaca
     3361 ttgacgcgac gttcctcaac gccgtgaaaa ccggggatcg ctcggctatc cgctccgact
     3421 atcgtgatgc gttgaagacg ctgaaagtca cgctcgcggc gacggaatct gcaagcaaaa
     3481 agaaaacgat cgtgttgaaa taacgttcca cgcactctgc attacgaaag gcacatttca
     3541 catggccgag atgctgttca cgttcgcctc gtacgccacg cggattgagg ggagtcttac
     3601 gccgctgaaa ggtatgaagc ggctcgcgga cacgcttcat ccgtatggcg tgaagatcac
     3661 ctggcttgta aaccacgatt cggccgaggc gttgaaaagc gacttgaccg aatggcacga
     3721 tcggtttggc gatgacattg cggtgatggt cccccatttc aagggcgggg caccggaact
     3781 tgtggcccag gcgcgggcgg cccgacagag gatgcagaag atcctcccgt ggacacgcgc
     3841 cgatattgcg gggaaccacg tgcgaagcag ggaatcgctg gaatcgctcg aagcagccgg
     3901 gttcatcggg ctgtggggat tctgctggga gcagatcgag gtggatgaca tcacggaccg
     3961 tggctgcccc tggggctttt actacctcga tccggcgttc cggaccgctc cgaacacacg
     4021 ctcgaaaggg ctgatcggca tggaatggac cgcgcgtgat ttgctcaagg cgtaccattc
     4081 aggtaatccg tgcatctaca gcacggatgt aaacgatgtc gcgcgcgccg gaatgtgcct
     4141 ctggcgggat atcgaatacc tcaagggcat gttcgacaac tactatcgga acctgcggta
     4201 caacgatctc gtcctgtacc aggttcatca ggaagcccac gagatgatgc cggaattccg
     4261 ctgttactcg gaagaggatt gccgggaagc agaagagatg ctggacgaac tcgcggatta
     4321 cgttgtcgga catgcacaca tcaggacggc cactctttcc gaagcggctg acgtgtaccg
     4381 ggagcggaac gatttcacag agccgagtta catgctctgg gaagacacgc cggctcctcc
     4441 gtacaacctt gattactcgc gaggtactcc gcggggcccg tggccaaaga cgttcctgtt
     4501 ctacgattcg gaatgccaga tgatgttcgt ggacggcaaa ttcgaaccgg tgtgcctcag
     4561 gaactacgcc gacagggaaa cgactgccag gcgatacttt gaggaggtgg atatcccgcg
     4621 ggtgcggctc cggtcgcaca cgcgatcacc ttacggaatg gagctgcacc tcaccgtcga
     4681 ggcgccgaaa gccatgccct tcggcatcac gatctgggag gacttcggac cctatctgct
     4741 cgcggaatct gtctctgagc cgacgggaac ggatctcatg gcgtccagaa tcctttcaca
     4801 ggagctgctt ctcctgcggt acaacgtgcc tgagggcaag acggaactcc gggtgaggtt
     4861 caaccggaag tagacgcatt cctgttccaa tgacgaaggg gcgcggtgca gccgcgcccc
     4921 ttccattttt gcccacacaa ccgatcaacc gcgccgtcag tttgtcacgc cagtttcgcc
     4981 agggcatccg cgtccgcata cacagccacg atttccccga aatacagggt gtgataatcg
     5041 ccactgcggt aggctgaccg gtcaatttca gccgaaagcg tctcctgaat cacgttgttt
     5101 ttctctacta cgcggcactc gtaatggatc acgcattctt cgacaatcgg tggggcaacg
     5161 cggcgacccg gtgttgctgt caggttctgt gcggcgaatt tgtcttcgtc acgtccggag
     5221 actttgccgc agtatgcaac cgcaccggca aggcttcgcg gcgggatgtt caccgtgaac
     5281 tcgggggtcg cctcgaggca ttcgtgcgtg taacgtgacg ggcggacgag cacgcaaaaa
     5341 atgggcttcc cccagatcca cccgaccgtt ccccacccga tagtcataac gttcgggcgc
     5401 ccgtttttcc cagtggagac gagaagggca cctccacgtg aaagcacttc gaggctttgc
     5461 gacaggtacg ccgtgaactg gatatcctgt ttggccatct ttctgtcctc cgtgcaccta
     5521 caggtgatac acatgggaga ggtcgcggcg cgtgatggcg ttgcgcgtat ccacaatcag
     5581 cctgctcttt tcggcgacgc gatcaaaatc aaacgcagtg tggtcggtga gaatgagtac
     5641 gcagtccgct tcccggagag cctcgtcggt caattcaacg cgttcgacag gttttccatc
     5701 gatggaaagt gagcggacgt ggggatcgca gtacgtgaca agagcgaact tcctccagag
     5761 ctcccggata atgcgcactg cgggagcttc gcgaacgtct tccacgtcct ttttgtagga
     5821 aacaccgaga acgaggattt tggacccgtt cacgcatttc ttctgccggt tcagcgccac
     5881 gatcactttt gtggtgacat agtggggcat catgtcattg atttcgccgg cgagttcgat
     5941 gaagcgcggc gagaaattca cctctttcgc tttccacgaa agatagaacg ggtcaattgg
     6001 tatgcagtgg cctcccagac cgggtcccgg gtaaaacggc atgaacccgt aaggcttggt
     6061 gcttgcggct tcgatgactt cccagatgtc cactccaagc ctgtcggcaa ggagcgccat
     6121 ttcgttgatc attgacacgt tgacgaggcg gaagatgttc tccagcaact tttccatctc
     6181 cgcgacacgc ggcgagctga ccctgtgcac ccggggattg aaggtgctgt aaagcgccgc
     6241 ggcaagctcg gtacactgtg gggtgacacc tccgaccact ttcggcgtgt tggcggtcgt
     6301 gaagtctttc cggccggggt caatccgttc cggggagaag gcgaggtaga aatcctttcc
     6361 cacgatgcgg ccggtcgctg tcagacgagg aagaatgatc tcctccgtcg ttccgggata
     6421 ggtagtgctc tcgagaacga taagctgacc gtctcgcagg ttttgcgcga ttgcgtcggc
     6481 ggtgctggcg acgtacgaga tatcaggtgt cttgtttttg tccagcggcg taggaacgca
     6541 gatgcatatc gcgtcgcact gacccagatc agacaggttg ttggtcgcgc ggaggcgccc
     6601 gctcttcgta aggggcacga ggcgttcgtc cggcacgtcg ccgatgtagg atacaccgtt
     6661 gttgacggaa tccaccttgg cgggttgcat atcgaacccg atcacgcgga ggcccgcccc
     6721 cgcaaactca actgccagcg gcaagccaac gtacccgagc ccgacgatcc ccacctgagc
     6781 ctgtcgcgat tcgatcagtt tttccagaaa atgcatgctt tttgttcgcc tttcgacgcg
     6841 aaaaagggct ggcgccccac ccgggaagcc agcccacccg caatgtgctc acgcaaagcc
     6901 accagatccc tctcagcgac gacccagccg ttccctgaac cgtgcgctca ggtcttcatc
     6961 gcggtcgtca tccggaacga ggtcgttgat ggcacggtcc acttcttccc atgtcagggc
     7021 ggcttcggtg gccttcatga tcggctcgat gagatgcgta tgccgcccca ccgcgcgact
     7081 gatgtacgct cttttttcct cttccgtgag gacgaaacga acgccgccgg tttcgagctc
     7141 cgctccccca gcttccacag catcctggtc aattcccaga tcatcgagaa acgcctgcgc
     7201 ttcttcgtgc gattcgtcct cggtgacggc ggcttgctcc ggcatgaaca gcgagtccac
     7261 gtcgagcatg tcgacaagag cgcggccctc gcacagctcc gcgactttgg ccgttccttc
     7321 cggcgtgccc cgcagaacca tcaccgcaag tgccaggtcc accggagctt caccgagttc
     7381 gtcttctgcg cggatgccaa gaaccagcag cgcgttgctg aggaccgaca ccgcagcgcg
     7441 gtccttcgaa ccctggaact ccttggcgtc cacatcgcgg aatacccttt gaaccgtatc
     7501 cgcggagaga agcctttttt cgtcatcctg tgccatcaac gagagcgcgc ggacgtagcg
     7561 ctcttcgaga ccgaggtggc tggcaagcac gacggcgctc tttgcggcaa ctttttcctg
     7621 ccggacgaag taattggctt tcaggctatc aatcggtgaa accaaagatt ccatctgcag
     7681 agacagcgcc cggttgatca actggtgcaa ctccttgcgc gaaagcacca tgcatttccg
     7741 cagggctgca tcccaggttg ccacggcggt ctggagagca gggtcggcat agtcgaagcg
     7801 ctggtcgttt ttccggcgtt ccagttcttt tcgccagaag ctgtcctcca cagagcgaag
     7861 aacccgcagc gccattgtgg gcagtgcttt gccaagcaat tcgggaaact tgatggtttc
     7921 cccttcaggc gctatctgcg cgacgtaacg ctccgtcgcc cttccaactg ccagtttgat
     7981 ggcttcgcgc gcctgttctg aagatgggat ttccatgatc aacctctcta cggcgcagtt
     8041 gcgccacaca cgcggagttg cacgctcaga taccccccag caccttgtcg agttctgcca
     8101 gcgcggaatc ggcaaacccg cccccgacgc tcaaaccctt ctgatcaccc ccttcgccgg
     8161 ggacgagatt cgccgagatc tccttcaccg ttttttccgc gaaaatcttc accattccaa
     8221 cggttgtgcc gcgcgcaaaa atcgagacga gtatagcctt gtcttccacc gcgcttacgt
     8281 acaggtcctt ctccttgccc tgtatgtaga acgcattgaa ctctttttca cccatctggc
     8341 cggcgatttg ttgagtcgaa gcgaagttgg ccgacaccag cgcgccgaac gatacgggat
     8401 ctatcgtatc catggcacca gactggcata cgaggtgtcc atccgggtcc acaagcagca
     8461 ccgtcctgat tccggcgtct ttcacgtaac ccgccagaac actctcaatg gtctgcattt
     8521 gctccgcgga gagagagcct ccctgtttgt tcatcgtcta tgctccatct gcggcgatgg
     8581 gtggatgagc agtgaccgcg ccgggttatc gcgtgccctc tttttcgagc ttttccagaa
     8641 ccatacgcgt aaccgccctc aatgcttcga acactccgcg ccccgtcagc gcttccattt
     8701 cgaagttcgg gacgcccctt ggattcatct tggcgttcag ttcctccacc gacatcacgt
     8761 caggcagatc ccgtttgttc cagccaatca caaacggcat ggcatcgagg tccataccgt
     8821 gttccttgag attctcccgg aggttttcca gactttccac gttgtcgtcg taccgatcct
     8881 tctggctgtc cgcgacaaac acgacaccgt cggcaccacg cagtacaagc tttcgcgtgg
     8941 cgttgtaata cacctgcccg gggactgtgt aaagctggaa cttcaccttg aaacctttga
     9001 tcgttcccag atcgagtggt aacaagtcaa agaacaatgt ccggtccgtt tttgtcgcga
     9061 ggctcgtcag cttccctctg ttcgtcgggg ggaccttttt gtgtattgtc tccagattcg
     9121 tggttttgcc actcataccg ggaccgtaat agacgatttt gcagttgatt tcccggtagc
     9181 cgtaattgat cgttgccatg aatcaccctc ccacgaaacc tgaatgtgtg tcaagccgac
     9241 tcgccggtca ctttgtcgaa ttgctgctgg actgcacgca cggttgcaaa gaggtcgccc
     9301 cagtccgcaa ccgggctgac acgggtgatc agccatccga catgaagcca cgtgatgacg
     9361 agggaatccc gcgtgccatg aacaaccatg tccgtggggt ggtgcagcgt gatgttcttc
     9421 agttgcgccc ctgcatcggt gacgaactgg gcgacaaatg cgctccattg ctcatcggtg
     9481 gtctcctcgt cacgcatgga acctgacgcc gccttgccct tcaatgcgcc gatcagcagg
     9541 ccttcccggt cagacacaac aacacgcacg acgtccgggc gttccgcgag aggagtgacg
     9601 atttccagta cctgttcact cattgcccgg tccctccagc gcggcgacga gcaagcgggt
     9661 gcgggccgcc agttcgtcca tgttggatgc agtttccacc agacctgcaa ccagacgcaa
     9721 gtcgtccgca taggcagcgc cgagcgtcca tcctccgtct tctccgaaca cacgaatacc
     9781 cgtcaggtcg ccgagatcaa tgaactggtt catcttgatt gccagttccc agatggcctc
     9841 agatttcgtg cccagcgaat ccgctgacat tcgggaaccg cgtgccgtca tcgacacagg
     9901 ctgcccacct gccatcacgc atccggcaac aagcccgggt gtcgcaaaca agtaccgggt
     9961 ggcatccgat gccgcctcat ccgcccattt gagtgtttct acgagcgatt ggtcggcgtt
    10021 gcccgctgcc agttccgcca gcgctcgttt tctggcttcc accttgtcac ccgcacgaac
    10081 ggctgcaagc aagccagtca taccggcaag ccgtccgttt atggcttgaa gggatgcttc
    10141 cgtgtcgggc acctcatctc cgcggagcac tttcgccagc gtaaggcccg cgctgaccca
    10201 ggcgttctct ggctcttccg cagaccagga cttcagaacg gcatcaaaat cggcccagtt
    10261 ctccgcctcg attgccgcct gaagatcatt cagtcggtcg aataacgctg ccggcatcag
    10321 ttggctcctt cgggaatctg ggtaacctgt gaccagtcta tctcgggaaa cgcggattcc
    10381 aaagcgcttt ttgccgtctg gccgtctgcc ttggtggcaa agcgcccgac gccgacgcga
    10441 taccactgtg aaccgtcgtt tttcgtgaac ggccacacga gcgcctcgta ccctctccgc
    10501 tgccatttcg atttcgcggc attggcttca ccgagagtcg ggagggattc cacatggacc
    10561 gtgaaccgtc cccctgggct cgtgggcagt tgtggcgcgc caccggaggg tcgtggcgcg
    10621 ggaggtgctg gtgccggcgc aggggcagcc actgccggcg gaggcgccgg agtcgttgcc
    10681 tccgcagctg gtgccggcgc cggtggcgga gcgggttggg cgacacgatc cgcggcgggt
    10741 gccggttgcg gagtcggtgc cggagtgacg gccgcaggcg atccggtcgt accggtcgag
    10801 aggccggaga tcaattcctt cagtttcgga ccgaagaaga aggaggcaaa cgccaccgcg
    10861 atgaatgcaa cgattaccca cccgataagc ttctttctcc ggtgctcttt cgccatctcc
    10921 gcgatgacgt cgtcgccacc gatgtcggcg atggggcctg tccatggcgc gggctttcct
    10981 tcctcaccct cttcaccggc tgcggtggct tcgggttggc cgacggggac aggttcctcc
    11041 gctttttcct caacaacagg aggctcgagt atggcggagg agaccacttc ctccagcgaa
    11101 gcaggttcgg cttccttctg ctcttcgccc gcaccctctc gttcctcgac tatgaaatcc
    11161 gccagcaaat ccgccgtgac atcggcttcc tgcaggccgg caaacggttt ctcctcggtc
    11221 gggggtgttt ccgcaggcgg tggagcagcc gatggctccg gtggagcctt ttccgcagca
    11281 ggaggaggtg ccgccccgct cggaagaact gtcccgagga ggttgaggta atgtgtcgcc
    11341 gccatccgcg cgaggcccgg aaccaatgcg ggggggatct ctccgccctc caccgcggga
    11401 accttagcgg gctcggttgg agccttttcc acggcaggcg gagaaggtgc agggggcgct
    11461 ggctcagaaa ccggaggagg cggaggagcc gcggtgggtg ccgcagccgg cgcctccgcg
    11521 acaggttcca cggcggtgct tgcctcttcc tcacctttgc cccagagcgc cgacagcgcg
    11581 tcgtctaccg agtcgaattc ctgtttcgca ggcggcttga taggcggggc aactactttc
    11641 gggcccgctt tcgccgcggc tggttctggt ggaggagtcg gcgctggctc ggccttcggc
    11701 ggaggtggag cttccttctt ttccgcgggt ttctcttttt ccactttggc ctgcggttca
    11761 gtcgggccga ggatggagga aatcgcctcc tccacctcct ctggccttat ttggaggcct
    11821 ttcaaaggcg tggtcgattt tgcggtcggc tcttcaacct cttccttctg ctccctgggc
    11881 ggggaaggct gctgtttttc ccggggcgca ggggctggcg gaggtgccgg tgcggcggca
    11941 ggtgcaggaa ccgtttccgt ccggggttca aggtccagca gttcgggcga gatggcggcg
    12001 atcagctcgt tgagaatcgc cgtttcatca atcgcatcca cctgtttctg caggaattcc
    12061 ggaagcctca cgccgtccgc ccggcccggc ccgacgcgcg gagccggagg gttcgatttc
    12121 ttctgtggtg catccatgcg ctttgccccg ctggtaatag gcccaccgat ccgcgcccct
    12181 cgaagcaaca cgtagagggg gacaagacgg aggaaacttt tgagcatccg gaacgatgca
    12241 cagtgctcgc gcgctccatt ccttagccac gtgttcctca accgttcaat gtatggtttt
    12301 ttttcctgtc tgtctacccc ctccgtaccg aaacaaatga aattccgtac ttaggcggtt
    12361 gaaattcggg ttttgttctc gacgcacccg tattctgaac gggcaagaat cccctgtcac
    12421 agatctggca cggagctctc ctcaaagcgc tccgacagcg ttcccacacc ttgtacgctg
    12481 gtttgcggaa gtggtgccgg agcgaatggg aggtcccgaa cgtggtgttc cacccgacat
    12541 catagcctca tgttgcgccg taccagcggg aatagctccg gcccgtgcac ggccagaccg
    12601 acccggtggc gattcccggg gtgcggatgc agtacagcga tccgtcgccg cacgggagaa
    12661 gcatttcatt cctgccgtca ccgtcgacgt cgctcacaaa gggttgatga atgtagtccg
    12721 gaaaggtttc cgtccagagc gttgcgccat cggcacggac gacatacacg tccgcgtacg
    12781 gccggatggc cgggcggttg ccgtacccgc tcgggttgcc agtggtgagc aggaaatccg
    12841 gcaggccgtc tccgtcgaga tcggcggcaa cgggattttg tgcgtcaagg tatgcgcgcg
    12901 gtaaggggtc ggagtgcctc agcactccgg tagcaaggga gaacagtcga acctcctgcg
    12961 cgccgttatt cccgccgtcc cgcgtattgg tgataccggc ggccagcaac tcccctcgaa
    13021 ctctggcgac ggccaacccg ccgcacccgc acggaaccct gtacgccagt tcgccgtcat
    13081 cccggaaaca ccacagggag gatgtttccc tgttgtcgcg atgcacgaga tcaagacctg
    13141 cgatgacgaa ctcatccctg ccatcgtggt cgacatccgc gaggatgacc ggtgtagacc
    13201 ccaggtcttc cgggaacccg tccttccgcc agaggactcc gcctgatccg tcgagcgcga
    13261 ggacggacga ggtgccgtcc tgattgctgt actcggttcc cacgatgatg gcggggattc
    13321 ctctcaaatg cgcaagcgcg ggtctgccgt ggcaaatctg gacgcgatcg gaatactccc
    13381 accgcagatc accggtccgc agatcggaac agaagacgtt ggcggaatcc caccacgcga
    13441 tttccggcct tccgtcgccg tccagatccg cagcgatgca cgacccgttt cccgcatgat
    13501 gggaacggct gcgcacttcc cacaccacat caccgctttc cgcgcgcacc aaccttgcat
    13561 accccgggcg cgggcgggca ttgtcggagg aaccaaacga gtagaagagg cacagcccca
    13621 gaaatggatc gtcaatcagg ttcgggaaca cgcaggcgag acggtcctcc gccgcgtccg
    13681 caacctgtgt ctgccacacg gtttcgcctt cggaggaaag gcagaatatg gcggattcgc
    13741 gacgaccgga cacagtccga ataccggaga ggtagagacg cacctgcccg tccaccatcc
    13801 cggacacgat cccattgagc tggtaacctg tttctttccg ccagatcaga tgaggagtca
    13861 ttcgagtcct ttttttcgca ctatgaacgg gaattcgcct cgatcacgag tcatccggcg
    13921 cgtgtatcgt cttgctcccg cgccccgctt tcagttaggt atactaaggg atgtcgagat
    13981 cacacttttt ccggcccgag tctcgcaatg tgcccgggcc gtcgtttcgc aaaggatagg
    14041 atatggctga cactctcgca atacgggggg gaactcccgc ggttccgccc ggcctcaagt
    14101 tccgtatgtg gccggagatc acgaaagagg atgaggagtt ggtgctcgcg tcactgcacc
    14161 aggacagcca cgcgttcggg ccgcatgcgg acatgctgca gaaggagttc gcggcatgga
    14221 acggcaacac gttctgcatg gcaacgaaca gcggcaccgc cgcgcttcac atgtgcgttg
    14281 ccgcatgcgg tatcgggccc ggtgacgagg tcatcacgac cgcgctttcg tggacgagca
    14341 gcgccacgtg tattatccac cacaacgcca tcccgatctt cgtggacgtt gactggcgca
    14401 gcatgcacat ggacccgtcg aagatcgaag ccgcgatcac accctatacc cgtgccatcc
    14461 ttgtggttca ttactggggt gtggcgtgtg acatggacgc gatcatgagt atcgccagac
    14521 ggcataatct ggccgtgatc gaggacgcat gccaggcgca cggcgcgacg ttcaaaggga
    14581 ggaaggtggg tacaatcggg gacgtggctg cattcagcct caatcaaaac aaaaatctgt
    14641 gcggcggtga gggggggctt ttcgtcacca atgaccccgg acttttcgaa cgcgggaaaa
    14701 tggtgacgag tttcggggat atgcgcccgc cggattccgg aagagactac cacgcatacg
    14761 ggctcggatg gatgtaccga acgtccgatc tcccggccgc gtttgccctt tcgcagctcc
    14821 ggaaacttga ccggacgaac aaatgggcgc gggacaactg ggatatgctg gatgcgcgtc
    14881 tgtcagggac gccgaatctg gttcgctcct tcacttccga agaccgcccg acaaacggat
    14941 acgcctacgt tctgcgcgtc gagcctgcgt acgcacaaag acggggcgtg ccttcgcgga
    15001 aaatcaccga aggaatcgtg gccgcattga aggcggaagg tgtgaatttc tccatcgcga
    15061 actggctgct tccggcgcat gcggtgttcc aggcgaagaa cgcatacggc aaaggcgagc
    15121 cgtggacgag cgcgtttgcc cgcaaggacg tctcctacga tctcagccag tatcc
//
LOCUS       DAOSOO010000062        14866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4051_length_14866_cov_6.016609, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000062 DAOSOO010000000
VERSION     DAOSOO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4051_length_14866_cov_6.016609"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1374
                     /locus_tag="PLT86_06205"
     CDS             433..1374
                     /locus_tag="PLT86_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT36238.1"
                     /translation="MECFVGIDTSQERLDVYVEPTREQFSHTNDSAGIAELVKRLKAL
                     SPTLVVIEATGKLEIPFMNEALEACLPVVVTNPQRIRDFARSVGQSAKTDTLDARIIA
                     QFAEKVRPELKSLPDHDQQKLVELVVRRDQIVAMLAAETHRLHRVSPFMRTEIQQDVT
                     YLMSRLNEISEQVQQLVRNHPVWKERRKLLESVPGVGPATSACILACLPELGRCSNRQ
                     IAALVGVAPFNADSGTRKGQRFIRGGRSRVRKALYMATLVALRYNPVLSAFYTRLITA
                     GTRKKVALVACMRKLVVILNTMIRHQQPWRTQTATQT"
     gene            complement(1421..4225)
                     /locus_tag="PLT86_06210"
     CDS             complement(1421..4225)
                     /locus_tag="PLT86_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT36239.1"
                     /translation="MPTGYEMHVISNTHWDREWLYNFQDTRMFLVEFMDKLLNILDKE
                     PDYAAYLMDSQVIPIEDYLEVRPEARERVTKHIKNDRIMVGPWYTLPEEFNVSGESLV
                     RNLLWGVKKSQAYGKVTLTGYSPFSYGQTSQMPQIYRGFDIDTILFYHGIQPEETKSE
                     FIHEAPDGSRVYGSRMGSMARYNFYFGVWRPAVYGMKTPDRKYQWSKGWLPFHLAGPH
                     RYQGHHFLLDVKKEINEANLRQALADLKAEELKHARTNYLAYMQGLDSTEPDLLEVEL
                     IRKAKHLLEPGDTILHSSLPAYLAKVKEAVKDMDLTVLVGERRTPRALGQKVHLYGDV
                     TSSRSRMKQANTRTEYALQRLAEPFCVAASTFGAEYPQTLLDLAWKYLLQSHPHDSIA
                     GSGVDQIELDVLNRLDQARNIAEGVMRRALGAMQMRINNSSVAWDEIVLTVFNPSPYE
                     RDETVTAFVDLPDEVKIEYYTLHEATTGRRVDHQEVSRARQMAVARHLGDATMEMNSD
                     QIKLHFWAEKIPALGYRTYILKRHDEPTGTIGTLLAGARTLENEHLRVEVACDGTLTV
                     TEKATGRVYTGLNEFEDSGEAGHPWRHVAPAYDEVISSIGAPFTVSVVEAGPLSATLR
                     IEHRMQIPVKMEEDQSDTIRRLDANGDGARRSAERKEMVITSLVRLVRGAKSVEVTTS
                     FDNECKDHRLRVCFPTFINAKKSAAETPFDVVEREIDRPEGSPWALSPNPTHPMGRFV
                     DVSDGVNGLAVINDGLREYEVVDTKERRLCITLMRAYQVELTTVSWRWERHPEMELAQ
                     CPGKHTFTYRIYPHSGTWADGKTFLEVEKLNLPVEVAQACSHPGDLPQELSFFSIAPN
                     DLVLSGMKTAEDGNGIVLRVVNPTTRDQKVTVTAWKPIAAAELVQLNEKPVEKLSPAG
                     SSVSFAAGPKKIMTVRVVLA"
     gene            4453..4782
                     /locus_tag="PLT86_06215"
     CDS             4453..4782
                     /locus_tag="PLT86_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT36240.1"
                     /translation="MPYTQYMEFIEATAFSRHVHEYLSDTEYLGLQLHLMRYPEAGRV
                     VPGSNGVRKLRWSMAGRGKSGGVRVIYYFKKQDDEIWLLTIYDKRETENIPAHTLRQI
                     AKEIDDV"
     gene            4775..5062
                     /locus_tag="PLT86_06220"
     CDS             4775..5062
                     /locus_tag="PLT86_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT36241.1"
                     /translation="MSERQIGQEILDGIKEIKAHKAGKKALHTHVLSEPSPPHAIRAK
                     LKLSQSAFARSMGVSLRTVQEWEQGRRKPSGPATALLRIAEQKPEVFKALT"
     gene            complement(5046..6608)
                     /locus_tag="PLT86_06225"
     CDS             complement(5046..6608)
                     /locus_tag="PLT86_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT36242.1"
                     /translation="MRLVTARQMAAIDRRTIDHHGVPGIELMHRAGQAVVEELLARAG
                     SVAGERFVVLCGKGNNGGDGFVIARLLRENNAAVAVYLLGERERVTGDARSAMEEWRA
                     AGGEVATLTEETAVHEAAASWRDADYLVDALLGTGLSGEVQGLMRAAIEEVNRLRVPV
                     IAVDIPSGVAGDTGEVCGVAVNAMLTVTFGLPKVGQALYPGRRHCGTLSVADIGFPEA
                     AVREALEGEPPIFMTSKNDAARWMPKRQPDAHKGDAGRVMVVAGSVGMTGAAALASEA
                     ALRAGAGLVYLGCPESLNDILETKLTEVITLPLPEIRRRRCLALRAVGDIRRMLPQMQ
                     VLAIGPGIGRFRETVELVRRVVSESSVPMVIDADGLFALAGAGLFPLPQPAVLTPHYG
                     EFARMVGCEIADIQRDPVESCRRAAGEWEVTLLLKGAPTVVCGPSGETWVNPTGNAGM
                     ATAGAGDVLTGAIAGLIAQGVAPEVAARLGAFAHGMAGDFARDELGTHGMIAGDILRH
                     LPKAMKELTSAP"
     gene            complement(6698..7405)
                     /locus_tag="PLT86_06230"
     CDS             complement(6698..7405)
                     /locus_tag="PLT86_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT36243.1"
                     /translation="MIRAVIFDLDNTLVDFMAMKKQAVEAAAWAMVDSGLPMTIEEAV
                     AGVYRTYDERGIENQQVFDHFLTETLGQIDYKILAAGIVAYRKAREAALQPYPHVKMT
                     LTQLLRNGLKLAVLSDAPRREAWLRLYQLGLHHTFDVVVTFEDTGLRKPAKEPFERVL
                     SLLDVAPKEAVMVGDWPSRDVTGAKAVGLWTVWAQYGDTFGTHSTDADYEIATIRDIG
                     RVIESINARMGGSEQTR"
     gene            7983..9062
                     /locus_tag="PLT86_06235"
     CDS             7983..9062
                     /locus_tag="PLT86_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36244.1"
                     /translation="MKRTMTSKERVLTTLAHAEPDRVPINYHANAGIDRRLKEHYGLD
                     PKDGEGLAKVLGVDFRGVGAPYVGPKLHPDLPDRHVDMWGIHTRWIEHESGGYWDYCD
                     FPLKDADEEMIRAWPMPSPDDFDYSHVEEECKKKEHFAIHCGGAGLGDIINSSGMIRT
                     MEQVLVDLMTDDPAGLALIDRRLGILFEVTRRTIEAAKGRIDLMWMGEDLGTQYTPLI
                     SLELYRKHLRPRHQRFVDLAKHYNLPVIIHTCGSSSWVYEDFIEMGFTGVDTLQPEAA
                     NMSPEYLKRTFGGRLAFHGCISTAGPVAYGTVEEVVEDVRRTLEIMMPGGGYCLSPTH
                     ALQDNSPTENVVAMYDAAHRYGVYG"
     gene            9308..10801
                     /locus_tag="PLT86_06240"
     CDS             9308..10801
                     /locus_tag="PLT86_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36245.1"
                     /translation="MRRLLYNSDGTNMFIRPSPMAASDLLRYVDEVADSQVTTFLASP
                     NAGQNFYCPSRVGEYIGKEWSDSDLDAMARQEGRALLASVTRNVRDLIRAGNDPLATV
                     LARCRRRGLEAFITFRMNELHDVDRPDSPLISRFYREHPEWRLNEPEHGWLGLAPNFA
                     VREVRERRIAEIADLVERHDIDGLELDFQRFPAYFSHAEGVAETHAPLMTELVAEIRR
                     HTERAASRRGHSILLSARVPGTLQGCLKIGLDPVEWHRRGLLDFLTIAPFLRTLFRMP
                     VREFKAAFPGLPVYAGVEFSAQHHEGKARPMTPELYRGVAASLMAQGADGVSLFNMFC
                     ARENAEHSWEPPFPVLSEIGSLSTLAGTPKLYLVDRRSDFDQPADPVPVLPAAVTPDR
                     AATVSLFVAESDCHASECLMRIETEPALPGGALSVSLNGDLLPAGRTANRARVFPEPH
                     DETAQEAARWKEFALPPGAVRAGENHVQVASGEPCTVLGTELAVPGS"
     gene            11236..13983
                     /locus_tag="PLT86_06245"
     CDS             11236..13983
                     /locus_tag="PLT86_06245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT36246.1"
                     /translation="MSVSPNPDATATDTAQAPHQFRISRYFSRPGTHPFDEIEWDIRT
                     ATIGNEKGETVFEQRDVEVPKSWSQLATNVVVSKYFHGPKGSPQRERSARQLVTRVVD
                     TITEWGDKDGYFLDEEERETFRAELTAILINQRASFNSPVWFNIGVPGARPQASACFI
                     NSVRDEMESILDLAKTEGMLFKWGSGTGTNLSPIRSSREALSSGGTASGPVSFMRGYD
                     TFAGSIKSGGKTRRAAKMVILNIDHPDIREFILCKVEEEKKARILIEAGYDPGFNVPG
                     GAYDSVHFQNANNSVRVTDEFMRAVENDGEWQTHAVVGGRVMDTYRARDLMQWIAESA
                     HMCGDPGIQYDTTINNWHTCPNTDRIHASNPCSEYMFLNDSACNLASLNLMAFRDENG
                     EFDIRAFRHAIRVVITAQEILVGNASYPTPAIERNSHDYRPLGMGYANLGALLMARGL
                     PYDSDEGRAYAGAVTALMTGEAYRTSAHLAARKGPFAGFAANREAMLGVIRKHGDHVQ
                     RIDRSYVPEPLFRAAETSWREAYELGERHGFRNAQATVLAPTGTIGFMMDCDTTGIEP
                     DMALVKYKKLVGGGALKIVNTTVPLALATLGYTETEIRQIVEYIDKNDTIEGAPFIKD
                     EHLPVFDCAFKPVKGTRSIHYMGHVRMMSAVQPFISGAISKTVNMPETATVEEVRETY
                     MQGWKLGLKAIAIYRDNCKSTQPVSTKRTEKAAPAEPEWKPRRRRLPDERNAMTHKFS
                     IAGHEGYITVGMYEDGQPGEIFIVMSKVGSTIAGLMDAFATMVSLALQYGVPLKVLAD
                     KFSHSRFEPAGFTNNPQIPMAKSIMDYIFRWLALKFLPQEERPTNGDNGHNGDKPPIA
                     EDLSASTLETRRKAAIEAAEKAVFVQQGDAPLCPDCGSIMIRSGACYKCLNCGGTSGC
                     S"
     gene            14231..14764
                     /locus_tag="PLT86_06250"
     CDS             14231..14764
                     /locus_tag="PLT86_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOT36247.1"
                     /translation="MPDGTRSGNKISRGNAAQFFVAGELCRRGHAAVVTLGNTPNVDI
                     LCSNAAGTRFAHIQVKTFEPGNRTCSVGIKAETDFGSTFFWVLSGIPSFGSDREFEYY
                     IIPSREMARNVREGHEKWLATPGKNGQVHHDSNVRAVNLPPFSPFWGWDITSFRNRWD
                     LIEEVLKPVSGFPPSRE"
BASE COUNT         2839 a         4708 c         4572 g         2747 t
ORIGIN      
        1 agcgcaagac gtatgacggc cccatggcgc gtttctttgc ggcgtggtgg gcgggttcgt
       61 aggcacgggt gggccgggga accgtagggc gcgacgcatg cgtcgctacc cacaaatgcc
      121 catggtaggg cgcgttgtcc tgcgccgccg tgggcgacgg tgaacaaaga cgcacgcgaa
      181 gggccagggg caacgtaggg gcggaccggt gtgtccgccc gcgttggcac acaattcgaa
      241 cacgggcgga cacaccggtc cgccccttgt atcttggtag tgttctggtg aggatgtttt
      301 tggggttcgg tagatcgctc gcccctgggt cgcgaagacc gttggtagta ggatcgccgg
      361 acctctggtg gagtcgcagt tcgaacagtt acatgagccg ttgagctcgc atcacaagga
      421 tgaagagacg ccatggaatg ctttgtcggg atcgacacct ctcaggaacg gctggacgtg
      481 tatgtggaac cgacgcggga gcagttcagc cacacaaacg acagcgcggg tatcgcagag
      541 ttggtgaagc gtctgaaggc actcagcccc acactggtag tcatcgaagc gacagggaag
      601 ctcgagatcc ccttcatgaa cgaagctttg gaggcttgcc ttcctgtcgt cgtcacgaac
      661 ccgcagcgca tccgtgactt cgctcgatcg gttggtcagt ccgccaagac cgacacactg
      721 gatgcccgga ttatcgccca gtttgccgag aaggtccgac cggagctcaa gagtctgccg
      781 gaccatgatc aacagaagct tgttgagctc gtggtgagac gggatcagat cgttgcgatg
      841 cttgccgcgg aaacgcatcg tctgcaccgc gtcagtccgt tcatgcgcac agagatccag
      901 caggatgtga cctatctcat gtcacgcctg aatgagatca gcgagcaggt gcagcagctg
      961 gtcagaaatc atcctgtctg gaaggagcgc cggaaactcc ttgagtcggt accaggcgtc
     1021 ggccccgcta ccagtgcctg tatcctggcg tgtcttcccg aactgggtcg ctgttccaac
     1081 cgccagatcg ctgcgcttgt aggtgtggcg ccgttcaacg cggacagcgg aacaagaaag
     1141 ggacaacggt tcatccgtgg aggcaggagc cgtgttcgca aagcactcta catggccaca
     1201 ttggtcgcct tgcgctacaa ccctgtcctc agcgcgtttt acacccgcct catcactgct
     1261 ggcacacgca aaaaagtcgc cctggtcgca tgcatgagaa agctcgtcgt catcctcaac
     1321 accatgatcc gacaccaaca accgtggagg acccaaacag ctacgcaaac ttgacaacca
     1381 agacagttac tacccccaca aattgtgttc aatgcccgtt ctacgccaat accacccgca
     1441 cggtcatgat cttcttcggg ccggcggcga acgacacgct tgaaccggcg ggggagagct
     1501 tctccaccgg tttctcgttc agttgcacca attccgccgc ggcgatgggc ttccacgcgg
     1561 tcacggtcac cttctgatcc cgcgtcgtcg gattgacgac gcgcagcacg atgccgttgc
     1621 cgtcttccgc cgtcttcatg ccgctcagga cgaggtcgtt cggcgcgatg ctgaagaagg
     1681 agagctcctg cggcagatcg ccgggatggc tgcacgcctg cgcgacttcc accggcaggt
     1741 tgagcttctc cacttcgagg aacgtcttgc cgtccgccca cgtgccgctg tgcgggtaga
     1801 tgcggtaggt gaacgtgtgc ttgccggggc actgggccag ctccatttcc gggtgacgtt
     1861 cccagcgcca cgacacggtg gtcaactcca cctgatacgc acgcatcagc gtaatgcaca
     1921 ggcggcgttc cttcgtatcc accacttcgt attcccgcag gccgtcgttg atcaccgcga
     1981 ggccgttcac tccgtcggag acatccacga accggcccat cggatgcgtc ggattgggcg
     2041 aaagcgccca cgggctgcct tcgggacgat ctatttcgcg ttccaccacg tcgaacggcg
     2101 tctccgcggc ggatttcttc gcgttgatga atgtcgggaa gcagacgcgc aggcggtggt
     2161 ccttgcactc gttgtcaaac gacgtggtga cctcgaccga ttttgccccg cgcaccagcc
     2221 gcacgagcga agtgatcacc atctccttcc gctcggcgga gcgccgcgcc ccgtccccgt
     2281 tggcatcaag ccggcggatg gtatccgact ggtcctcttc catcttcacc ggaatctgca
     2341 tccggtgctc gatcctgagc gttgcgctga gcgggcccgc ctcaaccacc gacacggtga
     2401 acggggcgcc gatggaggaa atcacctcat cgtacgccgg agcgacgtgg cgccaggggt
     2461 gacccgcttc tcccgagtcc tcaaactcgt tcaggcctgt gtagacgcgg ccggtggcct
     2521 tctcggtgac cgtcagcgtg ccgtcgcagg caacttcgac gcgcaggtgc tcgttctcca
     2581 gcgtacgtgc gccggcaagc agggttccga tggtgccggt gggctcatcg tgccgcttga
     2641 ggatgtacgt cctgtatccc agcgcgggaa tcttttccgc ccagaaatgc agcttgatct
     2701 ggtcgctgtt catctccatg gtggcgtcgc cgaggtgacg tgcgacggcc atctgccggg
     2761 cgcggctgac ctcctgatgg tccacccgcc tgccggtggt tgcttcgtgg agggtgtaat
     2821 actcgatttt gacttcgtcc ggcaggtcca cgaaggcggt gacggtttcg tcgcgttcgt
     2881 agggggaggg attgaacacc gtgagcacga tctcatccca ggcgacggac gagttgttga
     2941 ttcgcatctg catggcgccg agcgcacgcc gcatgacgcc ttccgcgatg ttgcgcgcct
     3001 gatcgaggcg gttgagcacg tccagctcaa tctggtccac gccgctgccc gcgatggaat
     3061 cgtgcgggtg gctctgcagg aggtacttcc acgccagatc gagcagcgtc tgcggatatt
     3121 cggcgccgaa tgtgctggcc gccacgcaga acggctcggc gaggcgctgg agtgcgtatt
     3181 ccgtgcgcgt gttggcctgt ttcatgcggc tgcggctgct cgtcacgtcg ccgtacaggt
     3241 ggactttctg ccccagtgcg cgcggggtcc gccgttcgcc caccagcacc gtgaggtcca
     3301 tatccttgac cgcttccttc accttggcga ggtatgcggg caggctgctg tggagaatgg
     3361 tgtcgccggg ttcgaggagg tgtttcgcct tgcggatgag ctcgacttcc agcaggtcgg
     3421 gctcggtgct gtccagcccc tgcatgtagg caaggtagtt cgtccgcgcg tgcttcaatt
     3481 cctccgcctt gaggtcggca agcgcctgcc gcaggttggc ctcgttgatc tccttcttca
     3541 cgtcgagcag gaagtgatgc ccctgatacc ggtgcggtcc cgcaaggtgg aacggcagcc
     3601 agcctttgga ccactggtac ttccggtcag gggttttcat tccgtagacc gccgggcgcc
     3661 agacgccgaa gtagaagttg taccgcgcca tcgagcccat gcgggagcca tacacgcgcg
     3721 agccgtccgg cgcctcgtgg atgaactccg atttcgtttc ctccggctgg atgccgtggt
     3781 agaacagtat cgtgtcaatg tcgaacccgc ggtaaatctg cggcatctgg gaggtctggc
     3841 cgtaggagaa cgggctgtac ccggtcagcg tcactttgcc gtaggcctgc gatttcttca
     3901 cgccccagag caggtttcgc acgagtgact cgccgctgac gttgaactcc tccggcagcg
     3961 tgtaccacgg gccgaccatg atgcggtcgt tcttgatgtg cttcgtcacg cgctctcgcg
     4021 cttcggggcg cacttcaagg tagtcctcga tcggaatcac ctgggagtcc atcaggtacg
     4081 cggcgtagtc cggctctttg tccaggatgt tcagcagctt gtccataaat tcgacaagga
     4141 acatgcgggt atcctggaag ttgtaaagcc attccctgtc ccagtgcgtg ttgctgatca
     4201 catgcatctc gtagccggtc ggcatggcga gggtcctttc gtggaagagg tcggtgcggg
     4261 agtaatcaga cagcgttgtg caacggtgaa aaggtacagc ggggggaggg aagtggcaaa
     4321 cggagcgggg gggaggagcg ccggcaaaga aggtgagacg gatggcgtgt gcgtcgcggc
     4381 ccttcggata catcatgacc ttccacccgg gctcttgtct gaaggacatt tggagttgcc
     4441 aggcatacgg ctttgccgta tactcaatac atggaattca tcgaggcaac agccttctcc
     4501 cgccacgtgc acgagtacct ttcggatacc gagtacctgg gcctgcaact gcacctgatg
     4561 aggtaccccg aggcagggag ggtggttccg ggctcgaacg gcgtgagaaa gctccgctgg
     4621 tcaatggcag gcagaggaaa aagcggtggc gtgcgcgtga tctactattt caaaaagcag
     4681 gacgacgaaa tctggttgtt gaccatttac gacaaacgag agacggaaaa catacccgct
     4741 cacactctga gacagatcgc aaaggaaatc gacgatgtct gaacggcaga tcggtcaaga
     4801 gattcttgac ggcatcaagg agatcaaagc gcacaaggcg gggaaaaagg ctctacacac
     4861 ccatgtgctg tctgaaccgt ccccgcctca cgccatccgc gcaaagctga aactttcgca
     4921 atcggccttc gcacgaagca tgggggtcag cctgcgcaca gtgcaggaat gggagcaggg
     4981 ccgccgcaag cccagcgggc ccgccacggc gctgttgagg atcgccgagc agaaacccga
     5041 agtcttcaag gcgctgacgt aagctccttc atcgccttcg gaaggtgccg caggatgtcg
     5101 ccggcaatca ttccgtgggt gccgagttcg tcccgcgcga aatcccccgc catgccgtgt
     5161 gcaaacgctc cgagccgcgc ggcaacctcg ggtgccacgc cctgcgcaat caagccggca
     5221 attgcgccgg taagcacatc accagcgcct gcggtggcca ttcccgcgtt gccagtgggg
     5281 ttcacccagg tctcgccgga tggcccgcac accaccgtcg gcgcaccctt gaggagcagc
     5341 gtcacctccc actcgccggc ggcccggcgg cacgactcca ccgggtcgcg ctgaatgtcg
     5401 gctatctcgc agcccaccat ccgcgcgaac tcgccgtagt gcggcgtcag gacggcaggc
     5461 tgaggcaacg gaaacaggcc ggcaccggcc agcgcgaaca acccgtccgc gtcaatcacc
     5521 atcggcacgg acgattcgga aacaacgcgg cggacaagct ccaccgtctc ccggaatcgc
     5581 ccgatccccg gacctatcgc cagcacctgc atctgcggca acatccggcg gatatccccc
     5641 accgcgcgga gtgcgagaca tcgccgcctg cgtatctccg gcagcggcag ggtgatcact
     5701 tccgtcagct tcgtctcgag gatgtcgttc aggctctccg ggcaaccgag gtagacaagc
     5761 ccggcacccg cgcgcagcgc cgcttccgag gccagcgccg cggcgcccgt catcccgacg
     5821 gatcccgcca ccaccatcac acggccggca tcgcccttgt gcgcgtccgg ttggcgcttc
     5881 ggcatccacc gcgcggcgtc gttcttggac gtcatgaaga tcggcggttc gccttccagc
     5941 gcctcgcgca ccgccgcctc cgggaacccg atatccgcca cggagagcgt tccgcagtga
     6001 cgccggccgg ggtacaacgc ctgccccacc ttgggcagcc cgaacgttac cgtcagcatc
     6061 gcattcaccg caacgccgca gacctcaccc gtgtcgcccg ccacccccga cgggatatcc
     6121 acggcaatca ccggcacgcg cagccggttc acctcctcga tggccgcccg catcagaccc
     6181 tgcacctcgc cggagagccc ggtaccgagc aacgcgtcca cgaggtaatc cgcatctctc
     6241 caggacgccg cggcttcatg caccgccgtt tcttccgtca acgtggcaac ctcaccgccg
     6301 gcggcacgcc actcctccat cgccgaccgt gcatcacccg ttacgcgctc tcgttccccc
     6361 agcaggtaca ccgccacagc ggcgttgttc tccctcagca gccgcgcaat gacgaatccg
     6421 tcgccgccgt tgtttccctt gccgcacagc acgacgaacc tttcccccgc cacggacccc
     6481 gcccgcgcga gcagttcctc taccaccgcc tgcccggcgc ggtgcatgag ctcgataccg
     6541 ggtaccccgt ggtggtcaat cgtccggcgg tcaatcgccg ccatctgccg tgccgttacc
     6601 agccgcatgg gtagttcctc tgagtcgaga cggggaaacc ggtcactgag gctgtcgaag
     6661 tgccggtgtg cttcgatagg ctcagcaacc ggctatttca ccgggtttgt tcgctcccgc
     6721 ccattcgcgc atttattgac tcgattaccc gcccgatgtc gcgtatcgtc gcgatttcgt
     6781 agtcggcatc ggtggagtgc gtgccgaagg tgtctccgta ctgtgcccag acggtccaca
     6841 ggccgaccgc cttcgctccg gtcacgtccc gcgaaggcca gtctcccacc atgacagcct
     6901 ccttcggtgc cacatccaga agcgaaagta cgcgctcaaa cggctctttc gccggcttgc
     6961 gcagacccgt atcctcgaac gttaccacca cgtcgaacgt gtggtgcagg ccgagttggt
     7021 acaaccgcaa ccatgcctcc cgccgcggtg catccgagag caccgccagc ttcaggccgt
     7081 tccgcaggag ttgcgtcagc gtcatcttca cgtgcggata cggctggagc gccgcttccc
     7141 gcgctttccg gtaggccaca atccccgccg caaggatctt gtagtctatc tgcccgagcg
     7201 tttcggtgag gaagtggtcg aacacctgct ggttctcgat cccgcgctcg tcataggtgc
     7261 ggtagacccc tgccacggcc tcctcgatcg tcatcggcag gcccgaatcc accattgccc
     7321 acgccgctgc ttccacggcc tgcttcttca tggccatgaa atccaccagc gtgttgtcga
     7381 ggtcgaatat gacggcgcgg atcatgaact ggtctccctg acgggatact ggtccccctt
     7441 gcaggatacg ggtctccaga gctctcaaac gcgcggcgag cacgtgttgg aaaagtagca
     7501 cctgccagcg ctgaggacaa cgttatccgg ggtccccgat ttgcggcgag cagagcacca
     7561 gtgcgtgaac cggccgggta cggcgaagcg tgcgcgccga cgcgttgttg ccttccatcc
     7621 ttgaccgcgc gttcaagggg taattgtaga gggtgggcac aggagcccgc ccctgcaaaa
     7681 aagcttttcg tgcatttcgt gtggttcgtg gtaaaaaaca cgggccgaca cgcaggtcgg
     7741 cccctacggc ttcctctttc gcgttcttcg cgcgcgttct tgttgctccc aaaaatcccg
     7801 taccccgatt gaaccatttt cgcagaaacc cttgacacga acacgccgca tcgcctgtgt
     7861 catacagtgg ccacaacgac agggtagcgc gccgtcagaa cgccgtttgt ccgccagccg
     7921 gggcgtggta cgttctaccg tcccgaaagg gtgaaacgcc gattcgacaa ggggaacccg
     7981 ccatgaagcg cacgatgacg tccaaagaac gcgtgttgac gacgcttgcc cacgcggagc
     8041 ccgaccgcgt gccgatcaac taccacgcca acgcggggat agaccgccgc ctgaaggagc
     8101 actacggcct cgatccgaaa gacggtgagg ggctcgcgaa ggtgctgggg gtggatttcc
     8161 gcggcgtggg cgcgccgtat gtcgggccga agctccaccc cgacctgccc gaccgccacg
     8221 tggatatgtg gggcatccac acccgctgga tcgagcacga aagcggcggc tactgggatt
     8281 actgtgactt cccgctgaaa gacgccgacg aggagatgat ccgcgcatgg cccatgccgt
     8341 cgcctgacga cttcgattac tcgcacgtgg aagaggagtg caaaaagaag gagcatttcg
     8401 caatccactg cggcggcgca ggactggggg acatcatcaa cagcagcgga atgatccgca
     8461 ccatggaaca ggtgctggtg gatctgatga cggacgaccc ggcgggtctc gcgctgatcg
     8521 accgcaggct gggcatcctt ttcgaagtga cccggcgcac catcgaagcg gcgaaggggc
     8581 gcatcgacct gatgtggatg ggagaggacc tcggcacgca gtataccccg ctcatcagcc
     8641 tggaactcta ccggaagcac ctgcggcccc ggcatcagcg cttcgtcgat ctggcaaaac
     8701 actacaatct gccggtgatc attcacacct gcggctccag cagctgggtg tacgaggact
     8761 tcatcgagat gggcttcacc ggagtggata cgctccagcc ggaagcggcg aacatgtccc
     8821 cggagtacct gaagcgcaca tttggcgggc ggctggcgtt ccacgggtgt atctccaccg
     8881 ccggcccggt agcctacggc acggtggagg aggtggtgga ggatgtccgc cgtacgctgg
     8941 agatcatgat gccgggcggg ggatactgcc tctcccctac gcacgcgctg caggataact
     9001 ccccgacgga gaacgtggtg gcgatgtacg atgcggctca cagatatggt gtgtacgggt
     9061 agtttgaaga gcctcacacg aagccgcgaa gattgcgaag catagacgag gctataactt
     9121 cggggttttt cttcgtgcct tcgtgtcttc gtgcgaacag aaaaagaatc tcacacgaag
     9181 ccgcgaagaa cgcgaaggag tactgaggct tcctgaagaa attcttactt cgtgccttcg
     9241 tgtcttcgtg cgagcagaac agaatctcac gcgaagccgc gaagatcgcg aagaggaggg
     9301 ggattctgtg agacgtctgc tttacaacag cgacggcaca aacatgttca tccggccatc
     9361 gcccatggcg gcaagcgacc tgcttcggta cgtggatgaa gtggccgact cgcaggtgac
     9421 gacattcctc gcgtcgccga acgccgggca gaacttctac tgcccgagcc gcgtgggcga
     9481 atacatcggc aaagagtggt ccgacagtga ccttgacgcc atggcgcggc aggaagggcg
     9541 ggcgctgctg gcctcagtca cccgcaacgt gcgcgacctc atccgtgccg ggaacgatcc
     9601 gctggccacc gtgctggccc gatgccgcag gcgcggcctg gaggccttca tcactttccg
     9661 catgaacgaa ctgcacgacg tagaccggcc cgacagcccg cttatcagcc ggttctaccg
     9721 cgagcacccg gagtggcggc tgaacgagcc ggagcacggc tggctcgggt tggcgcccaa
     9781 tttcgcggtg cgggaggtgc gggagcgccg gatcgcggag atcgcagacc tcgtggaacg
     9841 ccacgatatc gacgggttgg aactggattt ccagcggttc cccgcgtatt tctcacacgc
     9901 ggaaggagtt gccgaaaccc atgcgccgct catgaccgag ctggtggccg agattcgccg
     9961 gcatacggaa cgggccgcct cccgacgggg ccattcaatt ctcctttcgg cgcgcgtccc
    10021 cggaaccctt cagggatgcc tgaagatcgg gctcgacccg gtggaatggc accggcgcgg
    10081 gctgctggat ttcctcacca tcgcgccgtt tctgaggacg ctgttccgga tgccggtccg
    10141 ggagttcaag gcggcgtttc cgggccttcc ggtgtacgcg ggagtggagt tctcggcgca
    10201 gcatcacgaa gggaaggccc gcccgatgac accggagctg taccgtggcg tggcggcatc
    10261 cttgatggcg cagggagccg acggtgtatc cctgttcaac atgttctgcg cgcgggaaaa
    10321 cgccgaacac tcttgggaac cgccgtttcc cgttctcagc gagatcggca gcctctccac
    10381 gttggccggt actccgaagc tgtacctcgt cgaccgcaga agcgacttcg accagccggc
    10441 agaccccgtc cccgtgcttc cggccgcggt cacgcctgac cgtgcggcaa ccgtttccct
    10501 cttcgtcgcg gagagcgact gccatgcgag tgaatgcctc atgcgcatcg aaaccgagcc
    10561 cgcgctacct gggggagcgc tgtccgtttc actgaacggc gatcttctgc ccgcgggaag
    10621 aaccgcgaat cgggcccgcg tgtttccgga gcctcacgat gaaactgcac aggaggccgc
    10681 ccggtggaag gagttcgccc tgccacccgg ggcggtgcgc gcgggggaaa atcacgttca
    10741 ggtagcctcc ggcgaaccct gtacagtgct cgggacagag ctggccgtgc ctggaagctg
    10801 accgcccggc tctaccgtgg ctggcgggcg ttccgtggtt tcggcgggcg agtagtgctg
    10861 ctcaaaccgg cagatgtcgc gtaattccct gtccaacaac tgtttagctc caggattgag
    10921 cgcgctcccg cacaccgcaa attgcgggcc agaatgctac cggtagtggc gttcactttt
    10981 ttttgcccaa gatatcgccc cgtggccact tgcgcgtggc gcagcccaaa ccgccacccc
    11041 gtcggccatt attgctttct gcggtttttc gcatgcacgg gcagatcgcc ggatcatttt
    11101 ttccgcgccc gttgctgatc ttgacgacgc ggtccgggct ttctatcctc tctaggccca
    11161 ccgctagtgc aaaaatcctg aactttacac tatatattgt gtaccacggt tcttttgttc
    11221 agaaggagag tttgcatgtc tgtcagcccg aacccggacg cgactgccac cgacaccgcg
    11281 caggcaccgc accagttccg catctcgcgc tacttctccc ggcccggaac gcatcccttc
    11341 gacgagatcg aatgggatat ccgcacggcc accatcggca acgagaaggg cgagaccgta
    11401 ttcgaacagc gggatgtgga ggtacccaaa agctggagcc agctcgctac caacgtggtc
    11461 gtgtccaagt actttcacgg gccgaaaggg tcgccgcagc gtgaacgcag cgcacgccag
    11521 cttgtcacgc gcgtggtaga cacgatcacc gaatggggtg acaaggacgg ctacttcctg
    11581 gatgaagagg agcgggagac ctttcgggcg gagctgaccg ccatcctcat caaccagcgc
    11641 gcgtcgttca acagccccgt ctggttcaac atcggcgttc cgggcgcgcg cccccaggca
    11701 tcggcctgct tcatcaactc cgtgcgggac gaaatggaga gcatcctcga cctcgccaag
    11761 acagagggca tgctgttcaa gtggggctcc ggcaccggta cgaacctttc tcccatccga
    11821 tcctcgcgcg aagcgctttc cagcggggga acggcctccg gtccggtctc cttcatgcgc
    11881 gggtacgata cgttcgccgg ttccatcaaa tcgggtggaa agactcgtcg cgcggccaaa
    11941 atggtgatcc tgaacattga tcatccggat atccgcgagt tcatcctctg caaagtcgag
    12001 gaagaaaaga aagcgcgcat cctgatcgaa gcggggtatg atccggggtt caacgttccg
    12061 ggcggcgcgt acgattcggt tcatttccag aacgcgaaca actcggtgcg cgttaccgac
    12121 gagttcatgc gggcggtgga aaacgacggc gaatggcaga cacacgccgt ggtgggcgga
    12181 cgggtgatgg acacctaccg cgcgcgggac ctgatgcagt ggattgccga aagcgctcac
    12241 atgtgcgggg accctggcat ccagtacgac accaccatca acaactggca cacctgcccg
    12301 aacacggaca gaattcacgc atctaatccc tgcagtgagt acatgttcct gaacgactcg
    12361 gcgtgtaatc ttgcatcgct taacctcatg gcgttccgtg acgagaacgg ggagttcgac
    12421 atccgcgcgt tccgccacgc cattcgggtg gtgattacgg cgcaggagat ccttgtgggc
    12481 aacgcgagct accccacgcc cgccatcgag cgaaacagcc acgattaccg cccgctcggt
    12541 atgggatatg ccaacctcgg cgcgctcctc atggcgcggg gcctgccgta cgacagcgac
    12601 gaaggacgcg cctacgccgg ggccgttacc gcgctcatga cgggagaagc ctaccgcacc
    12661 tccgcccatc ttgccgcgcg gaaaggccca ttcgccggct ttgccgccaa ccgggaggcc
    12721 atgctgggcg tgatcaggaa acacggcgac cacgtgcagc gcatcgacag aagctacgtg
    12781 ccggagccgc tcttccgcgc cgccgaaacc tcctggcgcg aggcgtacga gctgggcgaa
    12841 cgacacgggt tccgcaacgc gcaggcgacc gtgctcgccc ccaccggcac catcggcttc
    12901 atgatggact gcgacaccac cggcatcgag cccgacatgg cgctcgtcaa atacaagaaa
    12961 ctcgtcggcg gcggcgcgct gaagatcgtc aacaccacgg tgccgcttgc gctggccacc
    13021 ctcgggtaca ccgagacgga aatccggcag atcgtcgaat acattgacaa gaacgacacg
    13081 attgaaggcg cgccgttcat caaggacgag cacctgccgg tgttcgactg cgcgttcaag
    13141 ccggtgaaag gcacccggtc aattcactac atgggccacg tccgcatgat gagcgcggtg
    13201 cagccgttca tctccggggc gatctcgaaa accgtcaaca tgcctgaaac ggcgacggtg
    13261 gaagaggtgc gggaaaccta catgcagggc tggaaactcg gcctcaaggc gatcgccatc
    13321 taccgggata actgcaagag cacgcagccg gtaagcacga agagaacgga aaaggccgcg
    13381 cctgcggagc cagagtggaa gccgcgccgt cggcggcttc ccgacgagcg caacgccatg
    13441 acgcacaaat tcagcatcgc cgggcacgag ggctacatca ccgtcggcat gtacgaggac
    13501 ggccagccgg gcgaaatctt catcgtcatg tcgaaggtcg gctccaccat cgccgggctg
    13561 atggacgcct tcgccaccat ggtgtcgctt gcgctgcagt acggcgtgcc gctcaaggtg
    13621 ctggcggata agttcagcca ttcgcgcttc gaaccggcgg gctttaccaa caacccgcag
    13681 atacccatgg cgaagagcat catggactac atcttccgct ggctcgcgct caagttcctg
    13741 ccgcaggaag agcgcccgac caacggggat aacggccaca acggtgataa gccgcccatc
    13801 gccgaggacc tgagcgcaag cacgctcgag accaggcgga aagcggccat cgaggcggcg
    13861 gagaaggcgg tgttcgtcca gcagggcgac gccccgctct gccccgattg cggctccatc
    13921 atgatccgtt ccggagcgtg ctacaagtgc ctcaactgcg gcggaacgag cgggtgttcg
    13981 tagggggaaa catcctggaa atcggtgttt tcgggtgtag gggcggttcg cgaaccgccc
    14041 ctacgcgcgt tttggggggt gcgcgtcctg aggttgtcgt tgcccgcgcg cgtttacaca
    14101 cgcaggccgc cgtacttctc ggtgtgacga ttggacaggt gggacaggca ttcctgcctg
    14161 tcatgggaaa gaaaactcgc cgggacgacc gaagggtcgc atttacagtc atcagtgggg
    14221 gaggtttcgg atgcccgatg gcacaaggag tggcaacaaa atttcccgcg ggaacgccgc
    14281 ccagttcttt gttgcggggg agctctgccg gcgcggccac gcagcggtgg ttacgctcgg
    14341 caatacgccc aacgtggata ttctgtgcag caatgctgct ggaacccgtt ttgcccacat
    14401 acaggtgaag acgtttgagc caggaaaccg cacctgcagc gtggggatca aagccgaaac
    14461 cgacttcggc tccacattct tctgggtttt gagcggcatc ccctctttcg gctctgaccg
    14521 ggaattcgag tattacatca ttccttcacg cgagatggcg cggaacgtgc gcgaagggca
    14581 cgagaaatgg ctcgccacgc ctggaaagaa cggtcaagtc caccacgaca gcaacgtacg
    14641 ggcggtgaat ctgccaccgt tcagcccgtt ctggggttgg gacatcactt cgtttcgtaa
    14701 ccggtgggac cttatcgaag aggtgctgaa acctgtgtct ggattcccgc cttcgcggga
    14761 atgacggcag tcttctcatc ttgcacacgg gtttgtctct ggattgcgca agcgcgcttg
    14821 cgctttgaaa gcggcagcgg ggctgccgca ctccaaagtc cgattc
//
LOCUS       DAOSOO010000063        14813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4073_length_14813_cov_5.362380, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000063 DAOSOO010000000
VERSION     DAOSOO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14813
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4073_length_14813_cov_5.362380"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PLT86_06255"
     CDS             complement(<1..1344)
                     /locus_tag="PLT86_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT36248.1"
                     /translation="MTSRSRLPRATVSQLERIVGRGFVIVDETLLQLYGETRGSDSIR
                     PLAILLPSTDEQVSEIFRLLGREGLGASVRGGGSGLVWGALPHDAVTVISTARMNTPL
                     RVVAEQMRVTAGAGVRIEEMVAAVTAHGFRLSGGTDMPPFATTGGFVARNGDGYGGPL
                     GLADGLLSGIRCVFASGDIVSSSQSGFNGPLDLHGFAVGSEGTLCVVTEATFRLEPAP
                     PREVIVVALFRDVVAATVAGVGLLKTGFLPDSADIIAAEAVRGALAGEAAVAAGAALL
                     VSVSGGEEDVEDGAQWVSGVCHDFLAMQVIVTDNRQRDEIANFWRSAQRQFCSHHGGV
                     LADFSCPLAALPELTGYALGQAREYGVVLSGMVRVASECLWMHGAAEQGDEAKEQAFL
                     ARFQEKALALKGAALAAHGVGARRLGFLPSLHREADLDAMRRIDAVLDKRAVLSLS"
     gene            1468..2343
                     /locus_tag="PLT86_06260"
     CDS             1468..2343
                     /locus_tag="PLT86_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT36249.1"
                     /translation="MFKLSVFTDEISQDFERAVAVASEYRLDGVEIRSVWDKPPQQLD
                     NRDIREIREILKGTGLKVASIASPFFKCHLGNAEERKEHIQILERCSRLADALECSII
                     RGFTFWRETPVENHWQDILAAFAEPLTLLEKYDKTLAIENEASTMVGTGRRLARFLGD
                     LNTDRVAAMWDPANCIYDDEDREIPFPDGYEAVKHRMRHMHLKDALRDPASNEPRCVP
                     IGEGLVDYRAQFPALVADGYDGFVSLETHWRPTALDEEQLNRPGGAAFSKEGEYATRV
                     CLDNLFRMLADLGLR"
     gene            2512..3759
                     /gene="glyA"
                     /locus_tag="PLT86_06265"
     CDS             2512..3759
                     /gene="glyA"
                     /locus_tag="PLT86_06265"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT36250.1"
                     /translation="MSNLRGVDPEVWALIQQEELRQTSKIRLIPSENYVSKAVLEATG
                     SVFTNKYSEGYAGNRYYEGQQNVDVLETLAQNRAKELFGARYVNLQPYSGSPANQAVC
                     HALLEPGDTIMGLDLRHGGHLTHGWKVNFSARYYRSVPYRVNPATGLLDYDALEALAN
                     EHRPKLIICGATAYSRIIDFPRIAEIARSTGAYSLADIAHISGLIVAGEHPSPVGVTD
                     VVSTTTHKSLRGPRGAMIMTNSEELAKKIDRAVFPGLQGGPHNNTTAAIAVALKEAAT
                     PEFREYGRQIVRNAKTLADALAKKGYNLISGGTDNHLILIDLTNKGISGKIAARALDL
                     GGIETNYNTIPDDPRKANDPSGLRIGTPAVTTRGMKEAEMRQIAEWMDLIIRNHDDET
                     LLDSTRNEIASFCEAFPAPGITT"
     gene            complement(3811..4740)
                     /locus_tag="PLT86_06270"
     CDS             complement(3811..4740)
                     /locus_tag="PLT86_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOT36251.1"
                     /translation="MPIRSRVAPIILLAGIAAAGCGNQVGDEQVREKQGEGASELAVV
                     SPVADVQGGAPGAMMATRGGIQNPVPSPMASMPVDTFRRQIIKTVDMTLEVSKVDSAL
                     AWAERIALEAGGYVGSVSRNEAPPEGARSGMITLKVPAGKTDECLSRLRALGALKNEH
                     IWTEDVTEDYYDTSIRLKNAQAAREQFEALLRQARRVPEIVEVQRELQRVTEEIERMT
                     GHLRRLSNMIAFSTVNLSVTEPVPILHDRTSVWGRLATALRGMVDAFWSTIAWLIRML
                     GVVIPVGLLIGAAWWSVRIYLKARTRGKSRNAG"
     gene            complement(4746..5660)
                     /gene="lipA"
                     /locus_tag="PLT86_06275"
     CDS             complement(4746..5660)
                     /gene="lipA"
                     /locus_tag="PLT86_06275"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT36252.1"
                     /translation="MPKPLPDTSEPNGEIEQGKRRLPRWFRVPAPGNERYREIQALLR
                     REHLHTVCEGAHCPNIGECWGIHGTATFMILGNCCTRRCRFCAVEKGTPSPPDISEAD
                     SVARAVQTLGLRHVVVTSVTRDDLPDGGASTFAEVIHRIRDANQDVTIEVLIPDFRGD
                     VESLKVVLEARPDVLNHNLETVRRLYAAARPQSDYERSLSVLRHASKQGCVTKTGIMV
                     GLGERWDELEELFADAARAGCSMMTIGQYLRPSEHHLPIDRFYTPDEFAMLRSSAEKA
                     GVSHVESGPLVRSSYHAAQQVNHATRER"
     gene            complement(5660..6451)
                     /locus_tag="PLT86_06280"
     CDS             complement(5660..6451)
                     /locus_tag="PLT86_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT36253.1"
                     /translation="MDLGLKGQVVMVCGASSGLGLAVAEEFAREGARIAICARSAGEL
                     ERQAARLSSTHSVEVVPVAADLSNPEGVNAAFTGVLDGFGGIDVLVTNAGGPPAVSFE
                     QTKDTEWEAAFQLTLMHVVRLSRLAAPVMRQRGGGRIVNLVSISVKQVVENLVLSTAI
                     RSAVVGLAKTLATELGPCGITVNNVCPGYIGTKRLESLWGGAAEKSGITYDEYCRNQA
                     KAIPLGRIGTPQDIAALVVFLASSRAGYMTGTTIQVDGGLYKGLL"
     gene            6591..7088
                     /locus_tag="PLT86_06285"
     CDS             6591..7088
                     /locus_tag="PLT86_06285"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT36254.1"
                     /translation="MQFADRVFLPLYEIVHDVSIGMVEAMPEEQIDFRPSPAVRSFRE
                     QIVHIASVERGMALGAAGQGWAFESEGFSPDDYPGRTALWNLLLQTRDEVISLARSIS
                     FHRLHAPVLTPWGFEATPAQILIFMRDHTNNHNGKLSVYVRMAGFEPPFFVALGSDTF
                     KQLVL"
     gene            7286..10771
                     /locus_tag="PLT86_06290"
     CDS             7286..10771
                     /locus_tag="PLT86_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36255.1"
                     /translation="MLIMCAGEFVVGGELKPGWERLVWNVPAAETLAVVLPEVVLVRD
                     SVEVETSDVTLISGEDFVVDYDHRRVVFLRPLPRGADVVIRYRRIPADWPVRQQWMRP
                     AQPDSADRDSIGAPRASVLARRQPLPESNLRIGGNKTFAVQVGSGRDLTLEQSLRVSI
                     NGTVAENVTVTAELSDQNLPIQPEGTTQELQEIDKVLVEIKSPHYRALLGSFDARNDI
                     GEFGRFSRRLDGAMGEASFDRWGASAGIASTRGEYRSLHLPIIDGNQGPYRLTDKNGN
                     TGIVVLAGTERVWVDGRLLVRGEPNDYTIDYSAGELTFTRKWLLRDDMEVVVDYEYSN
                     ESYARTTTLLSAYAQDGQRRGAWQTRVFFVQEGDDTGESFGVELDDTARALLAAAGDD
                     PAKASRPGWWYQPGGPYRLVDPSTEYFVKTTSDSGEYDVTFTRVGPGKGSYIRRHGYF
                     EQDMVYVGQGNGDWVPRITYPLPQRTRLTSLWTTYRPKSNFQMECEAAISDIDNNVLS
                     RVDDEDNTRFAGTISIETGLAPRDGQFGSINVRAKGRKIDEGFGGLYRTIDPYDNRRW
                     GLGVDESREQETTGEMTLTYRPGQNHTVDWDVGWLERNRLAERDGHTAWRTGLSWRTT
                     GDRMPSVRVSGEKIGMSISPVHGSVTSGDVFRGRAEVRAPVFRVTSSAKWEGEQDTRD
                     SPRERLYGTRFQDWSAGLAISETEHFAISMEVQSRYNSAYDSLFASWVRQQRSYTQTW
                     RGAVRSWKGLSSTAEYGWRKRQNLAGGGSTVSDNADVDIRHDALGGGVRNAFRYRIAS
                     TRTAKRERLFLYVGAGRGDYAPIDPNDTRAILAETEVVIAPAGDPTASYVLRYRDTDD
                     FSPTVTLDAAWQLNTDFNRFWKGAANPRTNEIRPLWQRAIRLVGTETNVLISETDTSR
                     TSDLYLLKLWTFRKLGKSPTLRGSVRVTQDLLLRDPSWAGDLRFRIMTYEDYDAALYS
                     RAGVPAVTNQKDYSVRGRLRVRPGTDWTAELKWQSDLRNGSNQDYSVRRNWLDQSITY
                     RNSSFDEFSLRNEAGEGKDRVPAAAYVAVQGKPLEAYVISLEPAWRKSWGNRGVMRTS
                     AQWSGVFVRNGTHGGMLPVHLLNGRNGGHNFRASMLASYRLSSLATLSVSYSARRNAG
                     TRLTHTARAEFNAVF"
     gene            10912..12048
                     /gene="ispG"
                     /locus_tag="PLT86_06295"
                     /gene_synonym="gcpE"
     CDS             10912..12048
                     /gene="ispG"
                     /locus_tag="PLT86_06295"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT36256.1"
                     /translation="MAHETPGSAGLGNDTSSHVKPLTLRTATRRVSVGGVQIGGGAPV
                     TVQSMCTTNTRDIDATVRQIHALADAGCEIVRVAVPHEADARALGEIRRQIPIPLVAD
                     IHFGHRLALVAVEQGVDKLRINPGNIGTRQSVERVASACSERGIPIRIGVNAGSLEKD
                     ILEKFGYPTAAGLVESALRHVRILEDLSFYDIVVSLKASDVPMAIAAYTDFSRERPYP
                     LHVGITEAGTAKTGTIASAVGIGAILSAGIGDTIRVSLTDDPVKEVAVGFDILKSLRL
                     RVKGPTLISCPTCGRVEIDLIRIAQEVERRLEKYSESFRVAVMGCVVNGPGESQGADF
                     GIAGGKGVGLVYRDGAVVRRVKEEELVAALFEEIEKARAEGRVS"
     gene            12048..13319
                     /locus_tag="PLT86_06300"
     CDS             12048..13319
                     /locus_tag="PLT86_06300"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOT36257.1"
                     /translation="MAIINPFRGIRYNSEKVGALQTVVAPPYDVISPEGRAQLLSKNL
                     FNVVRIDLPIGDGDAKYRYAGSLWRRWRESGVLVRDPAPALYFLEERYVTPSGRPSAR
                     RGFIGELVLEKIGKGSVLPHERTFAGPKADRLRLMEETLANLSPVFLLYRDPMDELGE
                     TYSRTASYPPDVEVETPDGTSRMWRVTDGDDIRRVAEVVRRYPLIIADGHHRYETAIR
                     FRDQQRSMGIRGAAESLMVYLCSSDDPDLTILPPHRVVTFPPSFDMDGFVSRLETYFT
                     VTAVDATSPNLRGLLGKSAGEPYEIGMWVKNRGSFVLQLRSWERVAEWIDPQRSLAWR
                     KLDVSLLHEVVLKRIAGVSENDMNIRYTVDPEDGCSAVVSGSADIFFLIRAPSAAQIA
                     EVAEAGDVMPHKSTYFSPKPLTGMVMRSLSD"
     gene            13337..14410
                     /locus_tag="PLT86_06305"
     CDS             13337..14410
                     /locus_tag="PLT86_06305"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOT36258.1"
                     /translation="MLTKGLLLCAGMGSRLRPITYTSAKHVLPVANKPLIHYTIEMLH
                     RAGITEIGIVVGVNRHEIEGAVGDGSRWGVKVDYIVQDNPLGTAHAVSLARDWAAGAP
                     FLMVLGDILVENGINSVVEAFAKNQPASVVVLHKTENPRQFGIAEIENGRIVGLEEKP
                     ESPKSNLAVAGIYVFQPCIFDVIGNVKPSLRGELELPDALRELIARNLVVQPLMLEGW
                     WKDTGRPEDMIDLNRLLLEKILEASCHGNVDGESSVVGRVVIESSAIVERSIIRGPVI
                     VGPGSTIRDAYIGPYTSIGPGVTIEGSEVEYSIIMEKSVISGIKSRIGSSLIGKNVRI
                     THERLMPSENRFVLGDSSLVNFA"
     gene            14466..14639
                     /locus_tag="PLT86_06310"
     CDS             14466..14639
                     /locus_tag="PLT86_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT36259.1"
                     /translation="MPAVVNKEECTGCGSCVEVCPSEAITMDDDVAVVNPEECTECGA
                     CVDECPSEAITLE"
     gene            complement(14703..>14813)
                     /locus_tag="PLT86_06315"
     CDS             complement(14703..>14813)
                     /locus_tag="PLT86_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT36260.1"
                     /translation="EALARAKGNKAEAARLLGISRRALYSRAASLGITLT"
BASE COUNT         3135 a         4362 c         4349 g         2967 t
ORIGIN      
        1 agaaagagac agaacggcgc gcttgtcgag caccgcatcg atacgtctca tcgcgtccag
       61 atcggcctct cggtgcagcg agggcagaaa acccagtcgt ctcgccccaa caccgtgtgc
      121 ggcgagagcg gcgcctttca gtgccaacgc cttttcctga aaccgcgcca aaaaagcctg
      181 ttctttggcc tcatcgccct gttctgctgc accatgcatc cagagacact cagaagccac
      241 tctgaccatt ccagaaagaa cgactccgta ctccctcgcc tgccccaagg catatcccgt
      301 cagctcagga agagccgcga ggggacagga aaaatccgca agcacaccac catggtgcga
      361 gcaaaactgc cgctgcgcgg aacgccagaa attcgcaatc tcatccctct gccggttgtc
      421 agtcacgata acctgcatcg cgaggaaatc atggcagaca cccgataccc attgcgcacc
      481 gtcttccaca tcctcctcgc caccggagac agacaccaac agcgccgcgc ctgccgcaac
      541 tgcggcttcg cctgccagag ccccccgaac ggcctccgca gcgataatgt ctgcgctgtc
      601 cggcaagaac cccgtcttca gcagacccac acctgcgacg gttgcggcaa ccacgtcccg
      661 aaacaacgct accacaatca cttctctcgg cggcgcgggt tcgaggcgaa acgttgcctc
      721 ggtgacgaca cagagggttc cttccgaccc cacggcgaac ccgtgcaaat cgagcggccc
      781 attgaaaccc gattgcgaag aagaaacgat gtcacccgat gcaaaaacgc accggatccc
      841 ggacaacagg ccatctgcga ggcctaaggg cccaccgtaa ccgtctccgt ttctcgcgac
      901 aaatcctcct gtcgtggcaa acggaggcat gtccgtcccg ccagaaagtc ggaatccgtg
      961 tgcagtcacg gccgcaacca tttcctctat ccgcactcct gctcccgcag tcaccctcat
     1021 ctgctctgca acaactcgca ggggtgtgtt cattcgcgca gtgctgatca ctgtaaccgc
     1081 gtcgtgtgga agcgcccccc agactagacc tgagccgccc ccgcgcaccg aagcaccaag
     1141 cccctctctc ccgagcaggc gaaaaatctc agaaacctgc tcatccgtgg agggcagcag
     1201 gattgccagc ggacggatgg aatcgctgcc tctggtttcg ccgtacagct gcagcaacgt
     1261 ttcgtcgacg attacaaaac cgcgtccgac aatccgttca agctgcgata cggttgctcg
     1321 cggcaaacga cttcgagacg tcacgggtct tcgtccaaaa ggatgcgctt gttcatatct
     1381 ccgcaacccg cgtatacttt ttcacaggcc gcgcaacgcc ctgccgggta acacaaagca
     1441 ctttggccac ctgaagggat atcctgtatg ttcaagctgt cagtcttcac ggatgagatt
     1501 tcgcaggact ttgaacgagc ggtagccgtc gcatcggagt accgtctcga tggcgtcgag
     1561 atacggtctg tctgggacaa accccctcaa caactggaca accgggatat ccgcgagatt
     1621 cgggaaatcc tgaaaggaac cggcctcaaa gtggccagca tagcgtcccc ctttttcaaa
     1681 tgccatctgg gcaacgccga ggaacgaaaa gaacacattc agattctcga gcggtgttcc
     1741 cgtctcgctg acgctcttga gtgctcgatt atcaggggat tcacgttctg gcgcgaaacg
     1801 cccgtggaaa accactggca ggacatcctg gccgcttttg ccgagcctct tacccttctt
     1861 gaaaaatacg acaagaccct ggcaattgag aatgaagcaa gcacaatggt cggaactggc
     1921 aggcggctcg cgagatttct gggcgatctc aacacggacc gtgttgctgc aatgtgggac
     1981 cccgcaaatt gcatctacga cgacgaagac agggaaatcc cgttccccga tgggtacgag
     2041 gcggtgaagc acaggatgcg ccacatgcat ctcaaggacg cgttgcggga ccccgcttcg
     2101 aacgaacccc ggtgtgtgcc aatcggcgag ggacttgttg attaccgcgc tcagttcccc
     2161 gcacttgttg ccgatggtta cgatgggttc gtctcgcttg aaacccactg gaggccgaca
     2221 gccctcgatg aggaacaact gaaccgaccc ggcggggccg ccttcagcaa ggaaggcgag
     2281 tacgccacgc gggtctgcct cgataacctc ttccggatgc ttgcggatct gggcctcaga
     2341 taacatactc tggggcccac cgatacgccg cgccgccgcg ccctcgagtt tcgccttgcg
     2401 aagccggtcg cgcgaacggt acatttcagt tcttaccaca ggtggatcca ttttcccaga
     2461 tccaacggca tctggtcccc ggtggtgttt ctgtccccta aggagttttc aatgagcaat
     2521 ctgcgtggtg ttgatcccga agtgtgggca ttgattcagc aggaagagct ccgtcaaacg
     2581 agcaagatcc ggttgattcc ctcggaaaac tatgtctcca aagcagttct cgaggccaca
     2641 ggttcagtgt tcacgaacaa gtactccgag gggtacgcgg ggaaccgcta ttacgaggga
     2701 cagcaaaacg ttgatgtgct cgaaacactc gcccagaacc gcgcgaaaga gctgttcggc
     2761 gccagatatg tgaaccttca gccctattct ggatcaccag ccaaccaggc cgtatgccac
     2821 gcgttgctcg agccaggcga tacaattatg gggctcgatc tccgtcatgg tgggcacctt
     2881 acgcacggat ggaaggtgaa tttctccgcc cgttattacc gttcagtccc gtatcgcgtc
     2941 aaccccgcaa ccggcctctt ggactacgat gccctggagg cgttggcgaa tgaacacagg
     3001 ccgaagctga tcatctgcgg ggcaacggcg tactcccgca tcatagattt cccccggatt
     3061 gccgaaatag cacgatccac cggagcgtat tcgcttgccg acatcgccca tatttccggt
     3121 ctgatcgtcg ccggggagca tccatcgccc gtaggggtta ccgacgtggt ctccacgaca
     3181 acgcacaaat cgttgcgtgg gccgcgcggg gcaatgatta tgaccaattc agaggaactt
     3241 gccaaaaaaa tcgaccgagc ggtgttcccc ggtctccagg gaggcccgca caacaatacg
     3301 actgctgcca tcgccgtcgc gctgaaagaa gccgccactc ccgagtttcg ggaatacggc
     3361 aggcagatcg tgcgcaacgc gaaaacactc gctgacgcgt tggcaaaaaa ggggtacaac
     3421 ctcatttcag gcggaacaga caaccatctg attctaattg acttgacgaa taaggggatt
     3481 tccggcaaaa tcgccgcacg cgcgctggac ctcggcggga tagaaacaaa ctacaacacc
     3541 atccccgacg acccacggaa agcgaacgac ccttccggtc tcagaatcgg aacgccggcc
     3601 gttacaactc gcggtatgaa ggaagcggaa atgcgtcaga ttgcggaatg gatggacctg
     3661 ataatccgca atcacgatga cgaaacgctg ctcgactcca cgcggaacga gatcgcatcg
     3721 ttctgcgaag cgtttcccgc gcccggcatc actacgtgat ggacgcccgc gctgcacaac
     3781 ggattcacaa gcccgcgggc ggttccggac tcaacccgcg ttgcgtgatt tccctcgtgt
     3841 gcgcgctttg aggtagatcc gaaccgacca ccacgctgca ccgatcagca atcctaccgg
     3901 gatcaccacc ccgagcatac gtatcagcca cgcgatagtg ctccagaacg cgtccaccat
     3961 tccccgaagg gctgttgcca gcctgcccca cacgcttgtt ctgtcgtgaa gtatgggaac
     4021 tggttcggta acgctcaggt tgaccgttga aaacgcgatc atgttcgaga ggcggcgcag
     4081 atggccggtc attcgttcga tttcctcggt cacgcgttga agttcgcgct ggacctcgac
     4141 gatttccggt accctgcgtg cctggcggag cagcgcctca aactgctcgc gggcggcctg
     4201 cgcatttttc aggcgtattg acgtgtcgta atagtcttcg gtgacatcct ccgtccagat
     4261 gtgctcgttt ttcaatgctc cgagagcgcg gagcctggac aggcactcgt ccgtctttcc
     4321 ggcgggcact ttgagcgtga tcatgccgct gcgggcacct tcagggggtg cctcgttcct
     4381 gcttaccgac cccacataac cgcctgcttc gagcgcgatc cgctcagccc atgcgagggc
     4441 tgagtcaact ttcgagactt ccagtgtcat gtccaccgtc ttgatgatct gacggcggaa
     4501 ggtatcgacg ggcatcgaag ccatcgggct cgggaccgga ttttgaatgc ctccgcgagt
     4561 tgccatcatg gcccccggtg cgccgccctg gacgtccgcg actggcgaaa ccacggccaa
     4621 ctcactggca ccttctccct gcttctcccg cacctgctcg tctccgacct gattgccgca
     4681 tcccgccgcg gcgatgccgg ccagaagaat gatcggggcg acacgcgatc tgatcggcat
     4741 tttgttcacc tttcacgagt ggcgtggttc acctgctggg ccgcgtggta tgaacttctg
     4801 accaacgggc cgctttcgac atggcttaca ccggccttct ccgcgctgct tcgaagcatc
     4861 gcgaactcgt caggggtgta aaaacgatct atgggaaggt gatgctcaga cgggcgcaga
     4921 tactgtccga tcgtcatcat tgaacacccg gcacgcgcgg catcagcaaa cagttcttca
     4981 agctcgtccc atctctcgcc caatcccacc atgataccag ttttggtgac acatccctgc
     5041 ttcgaggcat ggcgaagcac agagagggag cgttcgtaat cgctctgcgg ccgcgccgcc
     5101 gcgtacaacc gtcgcacggt ttccaggttg tgattcagca cgtccgggcg cgcttccaga
     5161 acgaccttga gcgattcaac gtccccacgg aaatcgggaa tcagaacctc gatggtgacg
     5221 tcctggttcg cgtcgcggat gcggtgaatc acctcggcga atgtacttgc cccgccgtcc
     5281 gggagatcgt ccctcgtcac ggaggtgact acaacgtggc gaaggccgag ggtctggact
     5341 gcccgtgcta cgctgtccgc ctcgctgata tccggggggg aaggcgttcc tttttctacc
     5401 gcacagaagc gacaccgacg tgtacagcag ttgcctagga tcatgaacgt ggcagtgcca
     5461 tgtatacccc agcattcgcc gatgttcgga caatgcgctc cctcacagac cgtgtggagg
     5521 tgctcgcgcc gaagaagagc ctggatctcg cggtaccgtt cattcccggg cgccggaaca
     5581 cggaaccacc gcggcagtcg acgcttcccc tgctctatct cgccgttggg ttcggatgtg
     5641 tctggaaggg gcttcggcat cacaggagac ccttgtacag accaccgtcc acctggattg
     5701 tggtgcccgt catatacccg gctcttgacg acgcgaggaa cacgacgagg gcagcaatgt
     5761 cctgcggcgt cccaatcctg ccgagaggaa tcgctttcgc ctgattgcgg cagtactcgt
     5821 catacgtgat tccggatttt tcagcggctc caccccagag ggattccagg cgcttcgtgc
     5881 cgatataccc gggacagacg ttgttcaccg tgataccaca gggtccgagt tccgtcgcga
     5941 gagttttcgc aaggccgaca acggcggaac gaatcgcggt cgaaagcaca aggttttcta
     6001 cgacctgctt cacgctgatc gaaaccaggt tcacgatgcg ccccccgccg cgctgccgca
     6061 tgactggagc tgcaagccgg gagagacgga cgacgtgcat cagtgtcaac tggaacgccg
     6121 cttcccactc ggtatccttc gtctgttcaa aactcaccgc aggcggaccg ccggcgtttg
     6181 tgaccaatac gtcaatccca ccgaatccgt caagcacccc tgtgaacgcg gcattcaccc
     6241 cctccgggtt cgagaggtct gccgctaccg gtaccacttc gacggaatgt gttgacgaaa
     6301 gccttgccgc ctgtcgttcc agttcgccgg cagaacgggc gcagatcgct atccttgccc
     6361 cttccctggc gaattcctcc gcaacagcca gccccaaccc actgctggcg ccgcacacca
     6421 tcacaacttg ccctttgagg cccaagtcca tattcttaca ccaacgagaa ggagtcttgt
     6481 gtgtttccgc acgctttgca cacgggtctt gtaattcatt cgcacgtctg aaatgtaacc
     6541 cttcggcgca cccgggaaca gaattgcgct ggagaacaca ggagtgcccc atgcagtttg
     6601 cggatcgtgt ttttctccca ctgtatgaaa tagtccacga cgtctctatc ggcatggtcg
     6661 aagcaatgcc cgaagagcag attgacttcc gtccgtcacc cgctgtgcgc tcgttccgcg
     6721 aacagatagt gcatatcgca tccgtggaac gggggatggc actgggtgcc gcaggccagg
     6781 gttgggcatt tgaatcagag ggattctcac ctgacgacta ccccggccgg actgcgctct
     6841 ggaatcttct tctccagact cgagacgaag tgatttcgct ggcgagatcg ataagttttc
     6901 accgcctcca tgcacccgtt cttacgccgt ggggattcga agccactccc gcgcagatcc
     6961 tcattttcat gcgcgatcac accaacaacc acaacggcaa gctctcagtg tacgttcgca
     7021 tggcaggatt tgaaccgcca tttttcgtcg cacttggctc ggatacgttc aagcaactgg
     7081 tcctttgaca tcgaacaccg cgccctttct gcatccggcc ggatggctgc gccgcggctc
     7141 gtccttttct caaacccatc ctttcacgat taatttcccg tctgcttctg cgattctgcg
     7201 ctcgcgggcg aggagccgga aggccaaatc ccccatctgc cggtgggggc ctcctcgcgt
     7261 ccgtttactg gcgtttttcg cgaccatgtt gatcatgtgt gcgggcgaat tcgtcgttgg
     7321 aggcgagttg aaacccggct gggaacggct tgtgtggaac gttccggcgg ccgaaacgct
     7381 cgcggtcgta cttcccgaag tggtgctcgt tcgcgattcg gtggaagtgg agacgtcgga
     7441 tgtcacgttg atctccggtg aagactttgt ggtggattac gatcatcggc gcgtcgtttt
     7501 cttgagaccg ttgccgcgcg gcgccgacgt cgttatccgc tatcggagaa ttcccgcgga
     7561 ctggccggtg cgacagcaat ggatgcgacc ggcgcagcca gactcggcgg accgagattc
     7621 gatcggcgca ccccgcgcgt ccgtgcttgc gcgccgccaa ccgttgcccg agagcaacct
     7681 gcgaatcggc ggaaacaaga ccttcgcggt gcaggttggt tcgggacgcg acctcactct
     7741 ggaacagtcg cttcgcgtca gcatcaacgg tactgtcgcc gaaaatgtca ctgttacggc
     7801 ggaattgagc gaccagaacc tgccaatcca accagaggga acaacacagg aactgcagga
     7861 gattgacaag gttctggtgg aaataaaaag cccgcactat cgtgcgctcc tcgggtcatt
     7921 cgacgccagg aatgacatcg gggaattcgg ccgtttttcc cgccggctgg atggggcaat
     7981 gggtgaggcg tctttcgacc gatggggcgc ctccgccggg atcgcctcca cgcgcggcga
     8041 gtatcgcagt ctccatcttc ccataattga cggcaatcag ggaccttacc gactgaccga
     8101 caaaaacggc aacactggta ttgtggttct tgcaggaacc gaacgagtgt gggtcgatgg
     8161 caggctgctt gtccggggcg aaccgaacga ttacacgata gattactcgg ccggtgagct
     8221 cacgtttacg cgcaaatggc ttttgcggga tgacatggag gtggttgtag actacgagta
     8281 ttcgaacgaa tcctacgcac gaacgacaac gcttctgtct gcatacgcgc aggacggaca
     8341 acgccgtgga gcgtggcaga cgcgcgtgtt tttcgttcag gaaggcgacg ataccggcga
     8401 gtctttcggc gtggaactgg atgacacggc cagagcacta ctggctgccg ccggagacga
     8461 tcccgcgaag gcatcacggc ccggctggtg gtaccagccc ggcggcccgt atcgccttgt
     8521 tgacccgagc accgagtact tcgtgaaaac cacttccgat agcggcgagt atgacgtgac
     8581 gttcacgcgc gtcggccccg ggaagggctc ttacatccgg aggcatggat acttcgaaca
     8641 ggatatggtt tacgtgggcc aggggaatgg cgactgggtc ccgcggatca cctaccctct
     8701 cccgcagcgc acacggctta catctctgtg gacaacctac cggccgaaga gcaacttcca
     8761 gatggaatgc gaagccgcga tcagcgatat cgacaacaac gtcctttccc gtgtcgatga
     8821 cgaagacaac acgcgctttg cgggaacgat ctcgatcgaa acgggcctgg cgccccgtga
     8881 cggccaattt ggttcgatca acgttcgcgc caaaggcagg aaaatcgacg aggggttcgg
     8941 cggcctgtat cggactatcg acccctatga caaccggcgc tggggcctcg gcgtggacga
     9001 gagcagggaa caggaaacta ccggagaaat gacgcttacg taccgcccgg gacagaatca
     9061 cacggtggac tgggacgttg gttggctgga acgaaaccgc cttgcggaac gcgacgggca
     9121 cactgcgtgg cgaaccggtt tgtcgtggcg caccacgggc gatcgcatgc cttccgtgcg
     9181 ggtttcaggt gagaagatcg gcatgagcat cagcccggtg catggttccg tgacatccgg
     9241 tgatgttttc cggggccgcg cggaagtccg agcccccgtt ttccgggtaa cttcatcggc
     9301 gaaatgggaa ggtgaacagg atacgcggga cagccctcgc gagcgtctct acggaacgag
     9361 gttccaggac tggtcggccg gacttgcgat ctccgagaca gaacatttcg cgatttccat
     9421 ggaagtccag tcgcggtaca acagcgcgta cgacagtctg ttcgcgagct gggtccgcca
     9481 gcagagatct tacacgcaga cgtggcgcgg cgccgtgcgc tcgtggaaag gtctttccag
     9541 tacggcggaa tatgggtggc gaaaacgtca gaatctcgcc ggcgggggca gcaccgtttc
     9601 ggacaatgct gacgtggata tccgccatga tgctctcggc ggcggcgtac gaaatgcgtt
     9661 tcgctaccgt atcgcttcca ctcgaacggc aaagcgtgag cgtctgtttc tctatgttgg
     9721 tgccggccgc ggcgactacg cacccattga cccgaacgac acccgggcga tcctcgctga
     9781 aacagaagtc gtcatcgcgc ctgccggcga tccaaccgct tcttacgttc tccggtaccg
     9841 ggacaccgac gacttcagtc ctacggtcac actggacgcg gcgtggcagt tgaacacgga
     9901 tttcaaccgt ttctggaaag gagcggcgaa tccgcggaca aacgagattc gtccgttgtg
     9961 gcaacgagcg atcaggttgg tgggtacaga gacgaacgtt ctgatcagtg agaccgacac
    10021 ttcgcgtacg agtgatcttt accttttgaa gctatggacg tttcgcaaac tagggaagtc
    10081 tccgaccctg cggggttcgg tgcgagttac ccaggacttg cttctccggg acccctcgtg
    10141 ggcgggagat ttgcggttcc gaatcatgac gtatgaagat tatgacgctg cattgtattc
    10201 ccgcgccggc gtgccggcag tgacgaacca gaaagactac tcggttcgtg gaagactcag
    10261 ggtgcgcccc gggacggatt ggaccgcaga gctcaagtgg cagagcgacc tgaggaacgg
    10321 gagcaaccag gattactccg tgcggaggaa ctggctcgat caatctatca cgtaccggaa
    10381 ttcgtcgttc gatgaattct cgttgcggaa cgaagcgggt gaaggcaagg atcgtgttcc
    10441 cgccgcggcg tacgtggctg ttcagggcaa gccgctggaa gcgtacgtca tcagccttga
    10501 accggcgtgg cgcaaatcgt ggggaaatcg tggcgtgatg cgtacctccg cacaatggtc
    10561 gggagttttt gtgcggaatg gcacgcacgg cggaatgctg ccggtccacc tgctcaacgg
    10621 caggaacggg ggccacaatt tccgtgcatc aatgctggcc agctatcgcc tttcatctct
    10681 agccacactg tctgtctcct attccgcccg tcgaaatgca ggaacacgat tgacgcacac
    10741 tgcaagggcg gaattcaatg cggtgttctg agaagcgaaa ccatttcggg aaatggacgg
    10801 agccgggcga agtgtcttat cttattactt tcccgcccgg gcactgcgtc gcggcattcc
    10861 cgaagcgcgt tttccagagt tgaatttctg cgtttttcac ggacacgttc catggcacac
    10921 gagacccctg gttccgcggg gttaggtaac gacacctctt ctcacgtgaa gccgcttacc
    10981 ctccggaccg caacccgtcg cgtttcggtc ggcggagtac aaattggcgg tggtgccccg
    11041 gtgacggttc agtccatgtg caccacgaac acgcgagaca tagacgctac cgtccgacag
    11101 attcacgccc ttgcggacgc cggttgcgaa attgtcaggg ttgcggtgcc gcatgaagca
    11161 gacgcgcgtg cgctcgggga aattcgtcga cagattccga tacctctcgt cgccgacatt
    11221 cattttggac accgtctcgc gctggtggcg gtggagcagg gagtggacaa gcttcgtatc
    11281 aacccgggca acatcggtac gcggcaatct gtcgaacgcg tggcgagcgc atgctcagag
    11341 cgcgggatcc cgatcaggat tggagttaac gcgggttcgc tggaaaagga catcctggag
    11401 aaattcgggt acccgacggc cgctggactg gtagaaagcg cgctgcggca cgtccgaata
    11461 cttgaggatc tgtcatttta cgacatcgtg gtatcgctca aggcgtcgga cgttccgatg
    11521 gcgattgcgg cgtataccga cttttccaga gagcgcccat atcctctgca cgtgggcatc
    11581 acagaagcgg gaaccgcgaa aacgggtacc atcgcttcgg cagtcggcat cggagctatc
    11641 ctgtcggccg gcatcgggga tacgatacgc gtgtctctca ccgatgaccc ggtcaaggaa
    11701 gttgccgtcg ggttcgatat cctgaaatcg ctgcgcctga gagtgaaagg cccgacgctg
    11761 atttcgtgcc ccacatgcgg gcgcgtggaa atcgacctga tcagaatagc tcaggaagtg
    11821 gagcgccgtc tggagaagta ctcggaatca ttccgggtcg cggttatggg gtgtgtggtc
    11881 aacgggccgg gtgagtctca gggggcagac tttggaattg ccggtggcaa aggcgtcggg
    11941 ctggtgtacc gcgacggggc ggtcgtaagg cgagtgaagg aagaggaact ggtggctgcg
    12001 ctgtttgagg aaatcgaaaa ggcacgcgcg gagggaaggg tttcttagtg gcgatcatca
    12061 acccgtttcg gggtattcgt tacaacagtg aaaaagttgg cgcactgcag accgtcgttg
    12121 ccccaccgta cgacgtcatc agtcccgaag gacgtgcgca actcctctca aaaaacctgt
    12181 tcaacgtggt gcggattgac ctgccgatcg gggatgggga cgcaaagtac cgctatgcgg
    12241 gaagcctctg gcgcaggtgg agggagtcgg gagttctggt gcgcgacccg gccccggcgc
    12301 tgtatttcct cgaggaacgg tacgtgacgc cctcagggag accttcagcg aggcgcggtt
    12361 tcatcgggga acttgtgctg gaaaaaatcg gcaaggggtc agtgctgcca cacgaacgaa
    12421 cgttcgccgg cccgaaggcg gaccgactcc gccttatgga ggagacactt gccaatctga
    12481 gtcccgtgtt cctgctctac cgggacccga tggacgaact cggcgaaacg tactctcgaa
    12541 cggcgagtta tccacctgac gtagaggttg aaacccccga cggcacaagc aggatgtggc
    12601 gggtaaccga cggtgacgac atccggcgcg ttgcggaagt agtacgccgg tatccgctta
    12661 ttatcgctga cgggcaccat cggtacgaga ccgcgataag gttccgcgac caacagcgca
    12721 gcatgggtat ccgaggggcg gcggagtctc tgatggtgta tctttgctcc agcgatgacc
    12781 cggaccttac tattctgcct ccgcaccggg ttgtgacgtt tccgccatcg tttgacatgg
    12841 atggatttgt cagccgtctc gaaacgtatt tcacggtgac ggcggttgac gcaaccagcc
    12901 cgaatctcag aggattgctc gggaaaagtg ctggagaacc gtatgagatc gggatgtggg
    12961 tgaagaaccg cgggagtttt gttctccagc tgcggtcgtg ggaacgcgtt gccgaatgga
    13021 tagatccgca gcggtcgctg gcctggcgga aactcgacgt gtctctcctg cacgaggtgg
    13081 tcctgaagcg cattgcgggt gtgagcgaaa acgatatgaa catcagatat acggtcgacc
    13141 cggaggacgg gtgctctgcc gtggtatcag gctccgctga catcttcttc ctgatacgcg
    13201 cgccgtcggc cgctcagatt gctgaggttg ccgaagccgg tgatgtgatg ccgcacaaat
    13261 caacgtattt cagcccgaaa cctctgacgg gtatggttat gaggtctctc agcgactgag
    13321 agcggaagga tgccgaatgc ttacaaaggg gttgctcctc tgtgccggga tgggaagcag
    13381 attgcgtccc atcacctaca cctcagcgaa gcacgtgttg ccggttgcca acaagccgct
    13441 gatccactac acgattgaaa tgctccaccg cgccggaatt acggagatcg gcatagtggt
    13501 gggggtgaac cgccatgaaa tcgaaggtgc ggtgggcgac ggttcgcgct ggggcgtcaa
    13561 ggtggactac atcgtccagg ataatcctct tggcacagca catgctgtat cgttagcacg
    13621 agactgggca gccggcgcac cgtttctgat ggttctcggc gatatcctcg tggagaacgg
    13681 catcaattcc gtggtggagg cgttcgcaaa gaaccagcca gcgtcggtcg tcgttctgca
    13741 caagacggag aacccgcgcc aatttggcat agcggaaatc gaaaatggga gaattgtagg
    13801 tctcgaagaa aaaccggaat caccaaaaag caaccttgcg gtcgccggga tttacgtgtt
    13861 ccagccgtgc attttcgatg tcatcggaaa cgtgaagccg tccctccgag gtgaactgga
    13921 gcttcccgat gcactacggg aactgatcgc ccggaacctt gtggttcagc cgctgatgtt
    13981 ggaagggtgg tggaaggaca ccggcagacc ggaagacatg atcgacctga accggttact
    14041 tctggaaaaa atcttggagg cttcgtgcca cgggaacgtc gacggtgagt cctccgttgt
    14101 cgggcgggtc gtaatcgagt cttccgcgat cgtcgaaaga agcatcatac gggggcctgt
    14161 cattgtcggc cccggttcga cgatccgcga cgcatatata ggaccgtata cctccatcgg
    14221 gccgggtgta acgatagaag ggagtgaggt ggaatacagc ataataatgg aaaaaagcgt
    14281 catttccggt atcaaatccc gtatcgggtc gagtctcatt ggtaagaacg ttcgcatcac
    14341 ccacgaacga ctcatgccgt cagagaatag attcgttctc ggggatagct cacttgtgaa
    14401 ctttgcgtag ccggcagacc cggaactcat tccgggagta ttcaactcaa ctcgggagca
    14461 agaaaatgcc tgcagtagtc aacaaagagg aatgcactgg ttgtggcagt tgcgtagaag
    14521 tgtgcccgtc cgaagccatc acgatggacg acgacgtcgc agtggtaaac cccgaagagt
    14581 gcacggagtg cggagcttgt gtggacgaat gcccaagcga ggcgattacg ttggagtagc
    14641 gtattccgca ccgggtggca tggcagcggc atgcccgttc gacctgccat ccggtgcgtt
    14701 cttcacgtca gagtaatgcc aaggctcgcg gcccgtgagt aaagcgctct cctgctgatt
    14761 ccgagcaacc gcgcagcctc tgccttgttt cctttcgcgc gggccaatgc ctc
//
LOCUS       DAOSOO010000064        14472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4200_length_14472_cov_6.217035, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000064 DAOSOO010000000
VERSION     DAOSOO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_14472_cov_6.217035"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLT86_06320"
     CDS             complement(<1..596)
                     /locus_tag="PLT86_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36261.1"
                     /translation="MAQTQVAVIGGGPGGYVAAFHAADLGLQVTLIDTDVNPGGVCLY
                     RGCIPSKALLHVAALINEADHAAAWGVTFARPTVELDKLRAWKQSVVQKLTGGLGELV
                     KRRKITHIQGRARFAGPTTLRVDKTSGGTEEVTFEYAILATGSRPTSIPTLSIPSPRV
                     LDSTGALELADVPESLLVVGGGYIGLELGTVYQALGSRV"
     gene            complement(628..2016)
                     /locus_tag="PLT86_06325"
     CDS             complement(628..2016)
                     /locus_tag="PLT86_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT36262.1"
                     /translation="MSKPNILIIMPDQQRADGMGCAGRFPIRTPNMDRLAEEGTRFAQ
                     AATVCPVCMPARASFINGLYPHNHGMWVNHGEMPAEDETFFHHLQRAGYYTAHVGKSH
                     YHEHRGGEHLREREPYMHARGLDYVHETTGPYATCRTPSYLTDSWAGTNYWELFKQDY
                     LERAKLGGAMVRPSPLPVDAFPDSYVGKQAVKFVNEYGGKQPFCMFVGFGGPHDPWDA
                     PGEYATMYDPASIPPPIPHSTQVDQFPGWMRNLVDFRTFGPEVQAQAAAIRANYFGKI
                     SLIDHWVGEILNAYAARGWLDDLLVVFWSDHGEMAGDHGRIQKCTFHESSLRVPLIIR
                     WPGRVPANTVSNALAEIIDVFPTLLETVGCEPSRRNQGKSLWQVLRGEQEDLREFQLS
                     EIDWQGRKIMIRSRRYKYAVDELGRGYMLYDLEKDPDEQNNLIADDAHRPLEACLREV
                     LLARLVGAQYTM"
     gene            complement(2060..3355)
                     /locus_tag="PLT86_06330"
     CDS             complement(2060..3355)
                     /locus_tag="PLT86_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT36263.1"
                     /translation="MATEVKIPDLGENGQGGQIVNVVAVQGEMIEIDQPMVELETDKA
                     TIEVPCPVGGKVVGVLVKPGDKVTVGQAVLSVDETEVPKEEAKPEPAPKEAAPVEPPK
                     PEPAAAPAEKAPEAPATRSEPEAGLLAPSPDTAPATPSVRRFAREIGIDITKVPGTGE
                     GGRITHDDVKAFSRAQNEARVVATAGSGIPAAALPDFEKWGALERQEMSNVRRKTAEH
                     MAFCWATIPHVTQFDTADITALEDWRKRAAKQVEAAGGKLTVTAVVLKIVGAALKAFP
                     QFNASIDVASNQIVLKKYVHVGIAVATDRGLLVPVIRDVDKKSITELSVELSEVSEKA
                     RSRKLGIEDMQGGCFTISNLGGIGGTYFTPIVNWPEVAILGLSRAQVQPVYVDGELKP
                     RLIMPLSLSYDHRAIDGVDGAVFVRWIARALEDPLTILL"
     gene            complement(3734..4477)
                     /locus_tag="PLT86_06335"
     CDS             complement(3734..4477)
                     /locus_tag="PLT86_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36264.1"
                     /translation="MAGLRDKLWLWGTGVNCLAKDYGFPESRMTIGGGLRELGIDQAM
                     MCGFLPPTEEEYRDVAFCRNLLWEMSFDDGFQFERPLAPILALHEAHPNVRGVLLDDF
                     STTEISKGAQPDLLARMREALPPGMQLWIVTYSMSLDIPNLAEYLRYADGISFWVWHA
                     RQLPNLAEYVARSNDLCGNKPTVVGLYFHDFGENRALSAREMAAQVESGVRLLEDGAC
                     AGLCFLSSSIMDIGLESVEWTKQWVRGLG"
     gene            complement(4489..6090)
                     /locus_tag="PLT86_06340"
     CDS             complement(4489..6090)
                     /locus_tag="PLT86_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOT36265.1"
                     /translation="MERLQIGDSAGWHFENGAWVDTDGGGIGIPQEIRRQSGDSLQGH
                     HYAFHLGADYGDFRARLRFSLTPHSDAGLIFHAVAPDDFFVLHFPECGQADRAQHFWA
                     AVSRMSGEGWLRIVRMELVRRVSSTTVASHEADIQLAGNRLFVRIDGRGVFDVTDPSF
                     VGYGRVGLFLFNNASIVDASVGGAKKSARVWPADKSPRTTWFHPVNGMEPGTWQRPGQ
                     IVRAGDDLLLSFTTQKPAYSGTTAEFVTRSTDNGRTWSPPERVTTHEAEAWDGWGYLH
                     RWPDGVLRMLISAETGARMAEGGPDGKLWRAPKPIPMPAAPSGLKRVYPAPLLNLSDG
                     SVLAFGYGGHDSTIPGSSIFTWGSCHCQAFAARTTDNGATWSNWVNIDGTRDSTGKTV
                     DGSLDLTEICGAEVAPGRIVALIRPVYSPWMWETWSEDGGRSWTPCVRGPFPGYATSN
                     MLRTTSGVLLVAHRLPGCTIHASHDDGVSWDNGTQIDSAIWVMGSMIEVEPDRVLYAY
                     WDSFESSMRAQFIRVTPRGIFPDAR"
     gene            complement(6302..6973)
                     /locus_tag="PLT86_06345"
     CDS             complement(6302..6973)
                     /locus_tag="PLT86_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOT36266.1"
                     /translation="MSEREPLIPVHGGYRHLKSFRVAQLVYDLTMRFCDSYVGKRSRT
                     HDQMVQAARSGVQNIAEGSQASGTSKKMELKLTNIARASLEELRLDYEDFLRQHGLPR
                     WERTDPRRQSLVDSRPATADDVARWAKRLHGEAEQGGRSGPGGLPKEIGASTTSITST
                     TRAVQKPPPTYPEIAANGALVLIAVAASLLDKQLAAQAGSFEKEGGFTERLHRVRSQN
                     RPNPA"
     gene            complement(7007..8512)
                     /locus_tag="PLT86_06350"
     CDS             complement(7007..8512)
                     /locus_tag="PLT86_06350"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT36267.1"
                     /translation="MVSLGEVLRSTAAKTPEKTAVVFQSRKTTYRALDRMTDGLAAHL
                     RNAGIEPGDRVALICINCAAFVVAYYGILKAGAVVVPINVLLNADEVRHIIADSGSRG
                     VIYHELLAPMVDGLGTALEEMRVKIRLSNDDEANAELFAPPKRAWKIPPINPVEDLAA
                     VMYTSGTTGRAKGAMMTHANLISNAAAYTVHMGVTSRDVTIVVLPMFHTFAATVGTNA
                     SIYIGATITVLVRFTPDEVARVMEEDGATVFMGVPSMYTIFCNLPEGKAPPLERLRVA
                     VSGGAALPVDIHRRFEERFGVGIREGYGLTECSPITTMTPITGVIKPGSIGPALEGVE
                     VRLVDDRGNDVPVGEVGELVVRGPNVMKGYWENEKATREAFFGEWFRTGDLARVDEDG
                     YYYIVDRKKDLIIVNGLNVYPRHVEEVIYQHPAVAEVAVVPEKDDLHGEVPRAVIALK
                     EGASATEREIIAFCRKRLGRFQVPRVVEFVPALPKNATGKILKRAIPPRVR"
     gene            8655..9116
                     /locus_tag="PLT86_06355"
     CDS             8655..9116
                     /locus_tag="PLT86_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOT36268.1"
                     /translation="MPPWAMLVLFLIVVFSVSVIGGLFTASSVGTWYEGLAKPSWRPP
                     NWLFGPVWTILYAMMAVSAWLVWKKAGFGSALLMFAVQLLINGIWSPVFFGAQSLGGG
                     LVVIVALWFAIVGTIAMFWPVSLAAALLLLPYLAWVSFATVLNAAIWRMNP"
     gene            9127..10020
                     /locus_tag="PLT86_06360"
     CDS             9127..10020
                     /locus_tag="PLT86_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOT36269.1"
                     /translation="MRVLLSGSTGLIGGELLWRLKAEGHHVTRLVRSPASFPEPVVVW
                     DCHNGPFDPAELSPIDAVIHLAGENISAGRWTAARKERIRSSRVDSTRLLAEAIARAA
                     QPPAVFIVASAVGYYGDRGNELVTEDSAPGTDFLAQVVLDWEAAAKPAAEAGVRVVHT
                     RFGVVLSSRGGALAKMLPLFRAGVGGRLGSGNQFMSWVALEDATAAIAHALTTDTLRG
                     PVNVVAPKPVTNREFTQALGAALGRPTFFVVPRVALRLVYGEMADATLLSSTRASAEK
                     LVSSGFSFRHPTLETVLNVLG"
     gene            complement(10828..11049)
                     /locus_tag="PLT86_06365"
     CDS             complement(10828..11049)
                     /locus_tag="PLT86_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOT36270.1"
                     /translation="MTKNEGSVDRIVRVILGVVLIVWAYVIGQGQWSTWSWILGIVGL
                     IALITGIIGWCGLYAVLGINTCKVPKKEG"
     gene            complement(11160..13166)
                     /locus_tag="PLT86_06370"
     CDS             complement(11160..13166)
                     /locus_tag="PLT86_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT36271.1"
                     /translation="MAFPGPGNTNSVSRSEADPAYLLGSIRTVLSDPYFGGIEITHIK
                     SHEDRRAVADLIREMRPNGMEITFACQPVQLINEENLIAPSDISSVDDNEREKAVARI
                     LSLLEEAAEVGATKLTLLSGRDPAYGIEDAKQAAEIREMGRVGIVRSLDTICRVAKPL
                     GITIVLEIFDNREEADGPKAFKGQFIGPTPDAVRLAENIRYERNYQNFGLLYDSSHMV
                     LMQETPEALKALKPYLAHVHVANCVTSRTAKDADQRYGDRHPKFGAAGSAVDSTVLAE
                     FVQALVEIDYRGTIGFEVAPIGSESPTDVIATAKAFFDEARNSVNVAYARPSGYAFRS
                     HRFFPEAALARVNEIRVEQPELVEELLSVRPRREKLTPDGRLTILAADHPARNVTQVG
                     DDPVAMGNRLDYLGRIMRVLVASEIDGLMATSDVIDDVVLADYVLQECGKPSVLEKRL
                     LIASMNRTGLAGAEYEMMDKMSSYRSAKRIAQMNLDGAKLLLRISAPDKYDRYVLQTI
                     DWCAEAIEQCNDLKLPVFLEPLPVERTETGYRTIKQPDPMIRVIGVAQALSHSTARTW
                     LKIPYTAEFDRVAKATTLPLLLLGGEATGRPWLTVEEFVRGLGAGANVRGALVGRNVL
                     FPGDEDPAVVAQAIHSVVHEHADAVSAMNAARERRGSMMDFFAL"
     gene            13358..>14472
                     /locus_tag="PLT86_06375"
     CDS             13358..>14472
                     /locus_tag="PLT86_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36272.1"
                     /translation="MLRTPFASERISMTDPTTGIRVIQLSSYPLPAAHFPYYWPSITP
                     DNRWILFYSQRFLQRNAPWDIFRVDADGLNLFQLTERNDVQETHGYYGRQYALLSHDG
                     KTLYVLWGNTLYAVDVETGNEELIACMDHLVGEGDALSYCVLSHEGRYIFFRKVSSFV
                     TSVRLDLRTGKAEDVELGGIAMGAMQTEPRVMVQAGEIIWGSEPTPDGGRRITNLGSK
                     PVLYSVAEDGSDRRLICPNIFAHCTLLGKTSKVQGCGLPPERCIWIAEEGSEPRKLCS
                     GPYFWHSGASYDGEWIVADTNWPDSGLQLVHVPTGHFRTLCHTGATLEHYEFGHSHPL
                     ISNDGRLVVFRSDRTGCPQVYVAHVSEEFRQSIIAGEL"
BASE COUNT         2487 a         4874 c         4387 g         2724 t
ORIGIN      
        1 acccgcgaac cgagcgcctg gtacaccgtt ccgagttcca gcccgatgta gccgccaccc
       61 accacgagca ggctttccgg cacatcggca agctccaacg cgccggtgga atccagcacc
      121 cgcgggctcg ggatggaaag ggtggggatg ctggtgggcc gcgatccggt agcaagaatg
      181 gcgtactcga acgtcacctc ctccgttcca ccggacgttt tgtccacgcg aagcgttgtc
      241 gggccggcaa accgggcgcg cccctgaatg tgagtgatct tgcggcgttt taccagctcc
      301 cccaggccgc ccgtcagctt ctgcaccacg ctctgtttcc aggcgcgcag cttatcaagc
      361 tccacggtcg gcctggcaaa cgtcacaccc catgcagccg catgatccgc ctcgttgatg
      421 agcgcggcaa cgtggagcaa cgccttggaa gggatgcagc cccggtacag gcacaccccg
      481 ccggggttca catccgtgtc gatcagtgtc acctgcaggc cgagatcggc tgcgtggaac
      541 gccgcgacgt acccgccggg ccctcctccg atcacggcaa cctgtgtctg cgccatgggt
      601 tcttcctttt ctcgccactc gtcgcgtcta catggtatac tgcgccccga caagccgcgc
      661 cagaagcacc tcgcgcaggc aggcttcgag cggcctgtgg gcgtcgtccg caatcaggtt
      721 gttctgctcg tcggggtcct tctccagatc atacagcatg tacccccgcc cgagttcatc
      781 caccgcgtat ttgtacctgc gggagcgtat catgattttt ctgccctgcc agtcaatctc
      841 gctcaactgg aactctcgca ggtcttcctg ttcccccctg agcacctgcc agaggctctt
      901 cccctgattt cgccgcgagg gctcgcaccc caccgtctcc agcagcgtcg gaaacacgtc
      961 tatgatctcc gcaagcgcgt tcgagacagt gtttgcgggc acccttcccg gccagcggat
     1021 gatgagcggc acccgcagac tggattcatg gaacgtgcac ttctggatgc ggccatggtc
     1081 acccgccatc tcgccgtggt ccgaccagaa taccaccaga aggtcatcca gccagccccg
     1141 cgcggcatac gcgttcagta tctcgccgac ccagtggtct atgagcgaga ttttcccgaa
     1201 atagttcgcg cggatggctg ccgcctgcgc ctgcacctcc gggccgaacg tccggaagtc
     1261 caccaaattg cgcatccagc cggggaactg atccacctgc gtggagtgcg gaatcggcgg
     1321 cgggatgctg gccgggtcgt acatcgtggc gtactcaccc ggggcgtccc acgggtcgtg
     1381 cggcccgccg aagcctacaa acatgcagaa tggctgcttg ccgccgtact cgttcacgaa
     1441 cttcaccgcc tgtttgccga cgtagctgtc cggaaacgcg tccaccggca gcgggctcgg
     1501 gcgcaccatc gcgccgccaa gcttcgcccg ctcgaggtaa tcctgcttga acagctccca
     1561 gtaattcgtt ccggcccagc tatccgtcag gtacgacggc gtccggcacg tggcatacgg
     1621 cccggtggtc tcgtgcacat aatccagccc gcgggcatgc atatagggct cgcgttcccg
     1681 caggtgctcg ccgccgcggt gttcgtggta gtgggatttg ccaacatgcg cggtgtagta
     1741 cccggcccgc tgcaggtgat ggaaaaacgt ttcatcctcc gccggcattt ccccgtggtt
     1801 gacccacatg ccgtggttgt gcggataaag gccgttgatg aacgacgccc gcgccggcat
     1861 acatacgggg cacacggtgg cggcctgcgc aaatcgcgtc ccctcctcgg ccagccggtc
     1921 catgttcggc gtgcggatcg gaaaacgccc cgcgcatccc attccatcgg ctctctgctg
     1981 atccggcatg atgatcagga tgttcggttt cgacatctgt cccctcccat cagggttgga
     2041 gcgtgaaccg gtatcggacc tacaaaagaa tcgtcagcgg atcctccagt gcccgggcaa
     2101 tccaccgcac gaacaccgcg ccgtcaacgc cgtcaatggc gcgatggtcg tacgagagtg
     2161 aaagcggcat gatgagcctc ggtttcagct cgccgtccac gtacaccggt tgcacctgtg
     2221 cgcgtgagag acccagaatg gcaacttccg gccagttcac gatgggcgtg aaatacgttc
     2281 ccccgatgcc accgaggttc gaaatggtga aacatccgcc ctgcatgtcc tcaattccga
     2341 gcttccggct cctcgccttc tccgatacct cgctcagctc caccgaaagc tccgtgatgc
     2401 tcttcttatc cacatccctg atgacaggca ccagcagccc ccggtcggtg gccaccgcga
     2461 tgccgacgtg cacgtacttt ttcagcacga tctggttact ggcaacgtca atactggcat
     2521 tgaactgcgg gaacgccttc agcgccgcgc ccacgatctt cagcaccacc gccgtcaccg
     2581 tgagtttccc gcccgccgcc tccacctgtt ttgccgcgcg cttgcgccag tcctccagcg
     2641 cggtgatatc ggccgtgtcg aattgcgtca catggggaat cgtcgcccag cagaacgcca
     2701 tgtgttcggc ggtcttccgg cgcacgttgg acatctcctg ccgctccagg gcgccccatt
     2761 tctcaaaatc aggcagcgcc gccgccggaa ttccgcttcc cgcagtcgcc accaccctgg
     2821 cctcgttctg cgcgcgcgaa aacgccttca cgtcgtcgtg ggtaatgcgg ccgccctcac
     2881 ccgttcccgg caccttggtg atgtctatcc cgatttccct cgcgaaacgc cgcacggaag
     2941 gtgtcgccgg tgcggtgtcg ggagatggcg ccagcaggcc ggcttctggc tccgatctgg
     3001 ttgcaggtgc ctccggcgct ttttccgccg gcgccgcggc gggttccggt ttcggaggct
     3061 ccaccggggc agcttccttc ggcgccggtt ccggttttgc ttcctctttc ggcacctccg
     3121 tttcatccac ggaaagcacc gcctgaccca ccgttacctt gtcgcccggc ttcaccagca
     3181 ccccgacgac cttcccgccc acaggacaag gcacctcaat cgtcgccttg tccgtttcca
     3241 gctccaccat cggctggtca atttcgatca tctcgccctg gacagcgaca acattgacaa
     3301 tctgcccgcc ctgcccgttt tccccgagat cgggtatctt cacttccgtt gccataagcg
     3361 cccgccttgc gttacggtgg tgggatcgtg acacgttcca tcggttgtga caaaatgtaa
     3421 gaccggaacc gggcgagggg taggacactt ccgcgattcg cgtgcatctt caccgtgtgc
     3481 ctgcgccctc cgacaccgtg ggctcggtct gttccgccgc ccctggcccc aatggccccg
     3541 tggatgccgt gttcgccgtg cgggtcaagg cggagcggcg gtaaccccgg cgaaacgaca
     3601 gggcaccgga cggcccatta cgtcgtacat gacccatagg accaatagca gaaaccggac
     3661 cgacctgcgc ggcgttcttt tggacccggt tgacagggtg gacacgcgtg ccaccagccc
     3721 gccccctccc acttcacccc agcccccgca cccactgctt cgtccattcg accgactcaa
     3781 ggccgatatc catgatggag gaactgagga aacagagccc cgcgcaggca ccgtcttcca
     3841 gcaaccgcac cccgctttcc acctgcgcgg ccatttctct cgcggaaagt gcccggtttt
     3901 caccgaaatc gtggaaatag agccccacca ccgtcggttt gttgccgcac aggtcgttcg
     3961 aacgggccac atactccgcc agattcggca attgccgcgc gtgccacacc cagaagctga
     4021 tcccgtcggc gtaccggagg tactcggcaa ggttgggtat gtcgaggctc atggagtagg
     4081 tcacgatcca gagctgcata cccggcggaa gcgcttcccg cattcgggcc agcaggtcgg
     4141 gttgcgcgcc cttgctgatc tccgtggtgc tgaagtcgtc cagtagcacg ccgcgcacgt
     4201 tcggatgcgc ctcgtggagc gcgaggatgg gcgccagcgg gcgctcaaac tggaacccgt
     4261 cgtcgaagct catctcccag agcagattcc ggcagaacgc aacatcgcgg tattcttcct
     4321 ccgtcggcgg gaggaagccg cacatcatcg cctgatcaat gccaagctcg cgcagcccgc
     4381 cgccgatggt catgcgggat tccgggaagc cgtaatcctt cgcgaggcaa ttcacgccgg
     4441 taccccagag ccagagtttg tcgcgtaatc ctgccatgat gcctctcctc agcgagcgtc
     4501 gggaaagatg ccccgcgggg tgacccggat gaactgcgcc cgcatggacg actcgaatga
     4561 atcccagtag gcatacaaca cgcggtcggg ctccacctcg atcatactgc ccatcaccca
     4621 gatggccgag tcaatctgcg tgccgttatc ccacgacacg ccgtcatcat ggctcgcgtg
     4681 aatcgtgcag ccggggaggc ggtgcgccac cagcagcaca cccgacgtcg tgcgcagcat
     4741 gttcgatgtc gcgtagccgg ggaacggccc gcgcacgcac ggcgtccacg accggccgcc
     4801 gtcctccgac cacgtctccc acatccacgg cgaatagacc ggccggatca gcgccacgat
     4861 gcgaccgggt gcaacctccg cgccgcatat ctccgtcagg tcgaggctgc catccacggt
     4921 cttgcccgtc gaatcgcgcg tgccgtcaat gttcacccag tttgaccagg tggcgccgtt
     4981 gtccgtcgtc cgcgccgcaa acgcctgaca gtggcacgag ccccacgtga aaatgctcga
     5041 gcccggaatc gtgctgtcgt gcccgccata gccgaaggca agcacggagc catccgacag
     5101 gttgagcagc ggagcggggt agacccgttt caacccgctc ggcgcggccg gcatggggat
     5161 cggcttcgga gcacgccaga gcttgccgtc tgggccgccc tcagccatgc gggcgcccgt
     5221 ttcggcggag atcaacatcc gcaggacgcc gtccggccag cggtgcaggt agccccagcc
     5281 gtcccacgcc tccgcttcgt gcgtcgtcac gcgctccggc ggcgaccacg tccggccgtt
     5341 atccgttgac cgggtgacaa actcggccgt cgtgccgctg taggcaggtt tctgcgtggt
     5401 gaagctcagg agcaggtcat ctcccgcacg gacaatctgc ccggggcgct gccaggtgcc
     5461 gggttccatg ccgttcaccg ggtggaacca tgtcgtgcgg ggggatttgt ccgccggcca
     5521 cactcgcgca cttttctttg ccccgccgac ggatgcatcg acgatcgacg cgttgttgaa
     5581 gagaaaaagc cccaccctgc cgtacccgac gaacgacggg tcggtcacgt cgaacacgcc
     5641 ccgcccgtca atccgcacga acaggcggtt gccggcaagc tggatgtccg cttcgtgcga
     5701 tgccaccgtg gtgctggata cacggcggac aagctccatc cgcacgatgc gcagccagcc
     5761 ctcgcccgac atgcgggata ctgccgccca gaagtgctgc gcacggtcgg cctgcccgca
     5821 ctcgggaaag tgcagcacga agaagtcatc cggcgccacg gcgtggaaaa tcagcccggc
     5881 gtcgctgtgc ggcgtcaggg aaaaccgcag ccgggcccgg aaatcaccat agtccgcgcc
     5941 gaggtggaac gcgtagtggt gcccctgcag gctgtcgccc gattgcctgc ggatttcctg
     6001 ggggatgccg atgccgcccc cgtccgtatc cacccatgcg ccattctcaa agtgccagcc
     6061 cgcgctgtcc ccgatttgca accgctccat ccggtgtacc tcctgaagag cctgttccgc
     6121 gttcccggtg cgtgttcata atggcgaatc tgtacgcgcg gcccaactcc tcgcactgat
     6181 ttgccctgtg gcccggctcg tatcagctat ggcgaggagg cgtcttcgtg tcaagtgttt
     6241 cgtccgaaaa tgtttcaatc gcggtacggg tgtttcggag agaaccgttc gggcgggcag
     6301 ttcacgcagg gtttgggcgg ttctgagacc gcacccggtg cagtcgctcg gtgaagccgc
     6361 cctccttttc gaacgatccg gcctgcgcgg ccagttgctt gtccagaaga cttgccgcca
     6421 cggcgatcag gacgagtgcc ccgtttgcgg cgatttcggg gtaggttggc gggggcttct
     6481 gaacagcccg ggttgtggac gttatggacg ttgtggacgc cccgatctcc ttcggtagtc
     6541 caccaggtcc acttcgtcca ccctgttctg cttctccgtg cagcctcttt gcccaccgcg
     6601 ccacgtcgtc tgccgtggcg ggccggctgt ccaccaggct ctggcggcgc ggatcagttc
     6661 gttcccaccg gggcaggccg tgctgccgaa ggaagtcctc gtagtccagg cggagttcct
     6721 caaggctggc acgcgcaatg ttcgtcagct tcagctccat cttcttcgag gtaccggacg
     6781 cctggctgcc ctcagcgatg ttctgcacac cactgcgggc agcctgaacc atctgatcgt
     6841 gggtgcggct gcgttttccg acgtagctgt cgcagaacct catggtaagg tcgtacacaa
     6901 gctgcgcaac acggaagctc ttcaggtggc ggtacccccc gtgtactgga atcagcggct
     6961 ccctctccga catcagcttg tcctccgcgt ccactccgtc cgccttttac cgcaccctcg
     7021 gcggaatggc cctcttcagg atcttccctg tggcgttctt cggcaacgcc ggcacgaact
     7081 ccacaacgcg gggcacctga aaccggccaa gacgtttccg gcagaacgcg atgatctccc
     7141 gctccgtggc gcttgccccc tccttcagcg cgattacggc gcgcggcact tcaccgtgca
     7201 ggtcatcctt ctccggcacc accgccacct ccgccaccgc cgggtgctgg tagatcacct
     7261 cctccacgtg ccggggatag acgttgagcc cgtttacgat gatgaggtcc tttttccggt
     7321 ccacgatgta gtaatacccg tcctcgtcca cccgtgcgag gtcccccgtg cggaaccact
     7381 cgccaaaaaa cgcctcgcgt gtggcctttt cgttctccca gtaccccttc atcacgttcg
     7441 ggccgcgcac cacaagctcg cccacctcac caaccggaac gtcgttcccg cggtcatcca
     7501 ccaggcggac ctccacccct tcgagcgccg gaccgatgga acccggcttg atcacgccgg
     7561 tgatcggtgt catggtggtg atggggctgc actcagtgag tccgtaccct tcccggatac
     7621 cgacgccgaa ccgctcttca aaccgccggt gaatatccac gggcagcgct gcaccgccgg
     7681 aaactgccac gcgcaggcgc tcgagcggcg gcgccttccc ctccggaagg ttgcagaaga
     7741 tcgtgtacat ggatggcaca cccatgaaca ccgtcgcgcc gtcctcctcc atcacccttg
     7801 cgacttcatc cggcgtgaac cgcaccagca ccgtaatggt ggcgccgatg tagatgctcg
     7861 cgttggtgcc gaccgttgcc gcaaatgtgt ggaacatcgg cagcacgacg atggtgacgt
     7921 cgcgcgatgt tacccccatg tgcacggtgt acgccgcggc gttcgaaatg aggttggcgt
     7981 gcgtcatcat cgcgcccttc gcgcggccgg tggtgccgga ggtatacatg accgcggcaa
     8041 ggtcttccac cgggttgatc ggcggtatct tccacgcgcg cttcggcgga gcgaacaact
     8101 cggcgttggc ctcatcgtcg ttgctcaggc gaattttgac acgcatttct tccagcgccg
     8161 tccccagccc gtccaccatc ggcgccagaa gctcgtggta gatgacgccc cgcgatcccg
     8221 agtcggcgat gatatggcgc acttcatcag cgttcagcag cacgttgatc ggcaccacca
     8281 ccgcgcccgc cttgagaatc ccgtagtacg ccaccacgaa cgccgcgcag ttgatgcaga
     8341 tgagcgccac ccggtcgccc ggctcaatgc ctgcgttccg gaggtgcgcg gcgaggccgt
     8401 cagtcatgcg gtcgagcgca cggtaggtgg tcttccgcga ctgaaacacg acggccgttt
     8461 tttcgggcgt cttcgccgcg gtggagcgga ggacttctcc aagagacacc atcgttcgat
     8521 attccttgtg cgggtgttgg agcgcgcggc acgggtgccg cgtacacaaa gataggccgc
     8581 cggccggccg ggtcaaaaaa agcgccacaa accccgaccg caggcgtaca accggggagg
     8641 gagctcctgt gaaactgcca ccgtgggcca tgcttgttct gttcctcatc gtcgtgttct
     8701 cggtttccgt aattggcggc ctgtttaccg cgtcatctgt cggcacatgg tacgaagggc
     8761 tcgccaaacc ctcctggcgg ccgccgaatt ggctgttcgg ccccgtgtgg acgatacttt
     8821 acgccatgat ggcggtttcc gcgtggctgg tatggaaaaa ggcaggattc ggctcggcgc
     8881 tgctgatgtt tgccgttcaa ctgctgatca acggaatctg gtcgccggtg ttcttcggcg
     8941 cgcagagtct cggcggcggg ctcgtcgtca ttgtagcgct ctggttcgcc atcgtcggca
     9001 ccatcgcgat gttctggccg gtcagcctgg cggcggcgct cctgcttctg ccgtaccttg
     9061 cctgggtgag tttcgccaca gtcctgaacg cggcaatctg gcggatgaat ccgtaaggag
     9121 gcagtcgtgc gtgttctgct gagcggttcc accgggttga tcgggggcga actgctgtgg
     9181 cggctgaaag cggaaggcca ccacgtcacc cgcctcgtgc ggtcgcccgc atcgtttccc
     9241 gaacccgtgg ttgtgtggga ttgccacaac ggaccgttcg atccggcgga actgagcccg
     9301 attgacgcgg tcatccacct cgccggagaa aacatcagcg ccgggaggtg gactgccgca
     9361 cgcaaggagc gcatacggtc gagccgcgtc gattccaccc gtctcctcgc ggaggctatc
     9421 gcgcgcgccg cgcagccgcc ggcggtgttc atcgtggcgt cggccgtcgg gtactacggc
     9481 gaccgcggca atgaacttgt caccgaggac tccgcgcccg gaaccgattt cctcgcgcag
     9541 gtggtgctgg actgggaggc cgccgcgaaa cccgccgcgg aggcgggcgt cagagtggta
     9601 cacacccggt tcggggtggt gttgagcagc cgcggcggcg cactggcgaa gatgcttccc
     9661 ctgttccgcg ccggcgtggg cggtcggctc ggttccggaa accagttcat gagctgggtg
     9721 gcactcgagg acgccacggc cgcgatcgcg cacgcgctga ccaccgacac actgcggggc
     9781 ccggtgaacg tcgtcgcacc gaagccggtc accaatcgcg agttcacaca agccctcggc
     9841 gcggcgctgg ggcgtccgac ctttttcgtc gttccgcgcg tcgcactccg cctcgtgtac
     9901 ggtgagatgg ctgacgcaac gctgctatcc agcacccgcg ccagtgcgga gaagctcgtg
     9961 tcttccggct tcagcttccg gcatccgacg ctggagacgg tgctgaacgt gctgggatga
    10021 ccgtcggggc tcgtagcacg ggcatcctgc ccgtgccggg cgcaaggttg ccggtgacgc
    10081 gagtacattc ctgtggagtt gcgcaccggt ccctgagcct gtcgaagggc cggcgcgcaa
    10141 agggagtctg agcacacttc atggcaactg cctggcaagg gttctggctg aaagtgctgg
    10201 gatgaccgtc ggaactcgta gcacgggcat cctgcccgtg ccgggcgcac gatttgccgg
    10261 tgactggatt ggtaacgctg cggtagtatg ttatatgtca aacggtcctg atcatagccg
    10321 ttccggtgtc accccaacgg taggaaaggg cattcccatg agagagaaac aaggacctcg
    10381 cataccgggg caggataagc gtggcggccc ggttctgcca agcaaagacg cgccgcacgc
    10441 gcaaccgaag ccgaaaatcc atcagaaagg cggcaccggc acgcggcata agtgagccgc
    10501 agcagtgaaa aaccgcttca ccggaggaga ttcttcactt cgttcagaat gacggtgctt
    10561 gcgcgacggg tgccagagaa ggcgtcattc tgagcgaagc gaagaatctc ccgccggcgt
    10621 tatacgcaac ccaccacagg cacccgctct tctgggcgcc cagcgccaac ggcgcgaaac
    10681 agaccagccc ggttcgcagg accgggcgtc ctgcggggcc ttggggtgtg cgtgcccgac
    10741 cgtccgtgcc cacagaaaaa gggcggggtg cgtccaatac acgccccgcc cttccggatt
    10801 cgtcgccgga cgttcgcgtc cccggcgtta tccctctttc ttcggcacct tacacgtatt
    10861 gatgccgagt acggcataca gaccgcacca gccgataatg ccggtgatga gcgcgatcag
    10921 gcccacgatc ccaaggatcc aggaccatgt ggaccactga ccctgcccga taacgtacgc
    10981 ccatacaatc agaaccacac cgagaatgac gcgaacgatc cggtcaacgg aaccctcgtt
    11041 tttcgtcatc attccctcct ttcggtcctg aggtggttac ctggccccgg cgtttcaccg
    11101 gggcaccggc tgtttgccgg aacgacgttt accagcattc tcgtacgata atatagcgtt
    11161 tacagagcga aaaagtccat catcgaaccg cgccgctccc gcgcggcatt catcgccgac
    11221 accgcatcgg cgtgttcgtg caccacgctg tggatcgcct gcgccaccac cgccgggtct
    11281 tcgtcaccgg ggaacagcac gttccggccc accagcgcgc cgcggacatt cgcgccggcg
    11341 ccgagcccgc gcacgaactc ttccaccgtc agccacggcc gtcccgtcgc ctcgccgccg
    11401 agaagcagca gcggcagggt ggtggctttc gcaacgcggt cgaactcggc cgtatacgga
    11461 atcttcagcc acgtgcgggc ggtagagtgg ctcaatgcct gcgcaacgcc gatcacccgg
    11521 atcatcggat ccggttgctt gatggtgcgg tagcctgttt ccgtgcgctc cacgggcagt
    11581 ggttcgagga acaccggcag cttgaggtcg ttgcactgct cgattgcttc cgcgcaccag
    11641 tcaatggtct gcagcacgta ccggtcgtac ttgtccggcg cggaaatgcg cagcagcagc
    11701 ttcgcgccgt cgaggttcat ctgggcgatg cgtttggccg agcggtagct ggacatcttg
    11761 tccatcattt cgtactcggc gccggccagg cccgtgcggt tcatgctggc gataagcagc
    11821 ctcttctcca gcaccgaggg tttcccgcac tcctgcagga cgtagtcagc cagtaccacg
    11881 tcgtctatta cgtccgacgt cgccatcagg ccgtctattt cgctggccac gagcacccgc
    11941 atgatcctgc cgagataatc gagccggttc cccatggcca ccgggtcgtc cccgacctgc
    12001 gtgacgttgc gggcggggtg gtccgccgcc aggatggtca ggcgcccgtc tggcgtcagc
    12061 ttttcacggc gcgggcggac ggagagcagc tcctccacca gttccggctg ttccacgcga
    12121 atctcgttca cccgggcaag ggcggcctcc ggaaaaaacc ggtgcgaccg gaacgcgtag
    12181 cccgagggcc gcgcatacgc cacattgacc gaattccgcg cctcgtcgaa aaacgctttc
    12241 gccgtggcga tgacgtccgt gggagattcc gacccgatcg gcgcaacctc gaacccgatg
    12301 gtgccgcggt aatctatctc caccagcgcc tgcacgaact ccgcaagcac cgtgctatcc
    12361 accgcgctgc ctgccgcgcc gaacttcggg tgccggtcgc cgtacctctg gtcggcgtcc
    12421 ttcgcggtgc ggctggttac gcagttggcc acgtgaacat gggcgaggta gggcttgagc
    12481 gccttgagcg cctccggtgt ctcctgcatg agcaccatgt ggctggaatc gtacagcagg
    12541 ccgaagttct ggtagttgcg ctcgtagcgg atgttctcgg caagccgtac cgcgtcgggc
    12601 gtggggccga tgaactgccc tttgaacgcc ttcgggccgt ccgcttcctc gcggttgtca
    12661 aagatttcca gcacgatggt gattccgagc ggcttggcca cgcggcagat ggtgtccagg
    12721 ctgcggacga ttccgacgcg ccccatttcc ctgatttcgg cggcctgttt cgcgtcttca
    12781 atgccgtagg cggggtcacg cccgctgagc agtgtcagct tcgtcgcgcc gacttccgcc
    12841 gcttcctcga gaagagacag gatgcgcgcc accgccttct cgcgctcgtt gtcgtccacc
    12901 gaggagatat ccgacggcgc gatcaggttc tcctcgttga tgagttgcac cggctggcag
    12961 gcaaacgtaa tttccatgcc gttcgggcgc atctccctga tcagatcggc aacggcgcgg
    13021 cggtcttcgt ggctttttat gtgggtgatc tcgatgccgc cgaagtaggg atcagaaagc
    13081 accgtgcgga tggagccgag gaggtacgcg gggtcggcct ccgagcggga gacgctgttg
    13141 gtgttccccg gaccgggaaa cgccatgaag tgaacaattg acagggtagt ctggtcgaga
    13201 gccatgaggc gctccttgag cagggcgatc gggaaacaga cccgccgaat acgagacgtc
    13261 cacgcgcact tgcgtacaac gtaaacgtac gggggaacac cccgttacac aagggatttg
    13321 cccgcagaca ccacaacccg aaggagaagc cgcccccatg ctccgcacgc catttgccag
    13381 cgagcgcata tccatgaccg acccgaccac gggtatcagg gtcattcagc tttccagcta
    13441 tcccctcccc gccgcccact ttccgtacta ctggcccagc atcacgccgg acaaccggtg
    13501 gatcctcttc tactcgcagc ggttccttca gcgcaacgct ccgtgggata tcttccgggt
    13561 ggacgccgac ggcctgaatc tgttccagct taccgaacgg aatgacgtgc aggaaacgca
    13621 cgggtactac ggccgacagt acgcgctgct ttcgcacgac gggaagacgc tgtacgttct
    13681 gtggggcaac acgctgtacg cggttgacgt cgagaccggc aacgaggagt tgatcgcctg
    13741 catggatcac cttgtgggcg aaggggacgc gttgagttac tgcgtgcttt cgcatgaagg
    13801 gcgttacatt ttcttccgca aggtgtcctc atttgtaaca tccgtgcgtc tcgacctgcg
    13861 gacggggaag gcagaggacg tggaactcgg cggcatcgcc atgggcgcca tgcagacgga
    13921 accccgcgtg atggtgcagg ccggggagat catctggggc tcggagccca cgcccgacgg
    13981 cgggcggcgg atcaccaacc tcggctccaa accggtgctg tacagcgtgg cggaggacgg
    14041 cagcgaccgg cggctcatct gcccgaacat cttcgcgcac tgcacgctgc tgggcaagac
    14101 gtcgaaggtg caggggtgcg ggcttccgcc ggagcggtgc atctggatcg ccgaggaggg
    14161 cagcgagccg cgcaagctct gctcgggacc ctatttctgg cattccggcg cgtcgtacga
    14221 cggggaatgg atcgtcgccg acaccaactg gccagatagc ggtctgcagc tcgtccatgt
    14281 gccgaccggg cacttccgaa ccctgtgcca cacgggcgcg acgctcgaac actacgaatt
    14341 cgggcattca cacccgctca tcagcaacga cggccggctg gtggtgttcc gctccgaccg
    14401 caccggctgc ccgcaggtgt acgtggcgca tgtgtcggag gagttccgcc agagcatcat
    14461 cgccggggag ct
//
LOCUS       DAOSOO010000065        14106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4339_length_14106_cov_5.726639, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000065 DAOSOO010000000
VERSION     DAOSOO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14106
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4339_length_14106_cov_5.726639"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLT86_06380"
     CDS             complement(<1..1044)
                     /locus_tag="PLT86_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT36273.1"
                     /translation="MILGAMAVFLAICAPKVMAAARGFYARLTILSTIVRLARDVYVD
                     EVDTDRLMDGAIDGLLDRLDPHSDYLPPEEAARFGERIRGSFGGVGIYYTIIEGLPTV
                     TSVIEGGPAEHAGLLSGDRILSVDGLTTVNWREAEVQEHLKGPVGTRVRLEIRRNGRE
                     TPLPVEIVRGVIPLKSVPYAFMLDDTTGYIRIANFAQTTGQEVEEAIGRLNDAGMRCL
                     VLDLRDNGGGELNAAVAVADFFLDAGSVIVSQRGRWEQANQTYFATSGSRKVDTPVVV
                     LINRGSASASEIVAGALQDTDRGIIVGQQSFGKGLVQRQFDLSQQAQGGGVLLLTVAR
                     YYTPTGRLIQRDYG"
     gene            complement(1089..2765)
                     /locus_tag="PLT86_06385"
     CDS             complement(1089..2765)
                     /locus_tag="PLT86_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36274.1"
                     /translation="MVESMRGENARSIEENAHSAPSLDLSVVILSYNVRAYLALAIRT
                     ALQATRSLAAEIIVVDNASQDGSADMVAADFREVRLIRSDRNLGFSGGNNLGIRIARG
                     RYILLLNPDVIVHPRAFEALVAYLDTHPSAGAAGGRVVNPDGTTDRGARRGFPSPSAA
                     FYRMVGLSFIFPKSRRFARYNMTYHDDGQECEVDAVSGCFMAVRREVVERIGLLDESF
                     FMYGEDLDWSFRIRSAGWTIGYVPSAEIVHFRGESTRSIARLRQLYLFHRAMHVFVRK
                     HIAPTRIPLLVWLIEFGIVVRGAAITLWRVLLAAVVPIADVAVLTAGFVLALFARTQT
                     GWTIPPFSIRQWATIWMVFVASGLLGAAATGLYVRNRLALRRAAVAAAIGAVASIITI
                     FLVRTINFSRIVTLVTWALGGGGMLLWRWLVSRASQPPRAGWLVLGCGEKAQRFLNSL
                     SSSEDRYHVLGVVRSEDDPIDCTAVAGFPVVGSASDLPILLRSFRVDDLIVAKEKYHY
                     HELLDLVRRGGRYPGRVRLVADDDVPVGAETPPDNRFMDLNLQKRTWFLQ"
     gene            complement(2758..3492)
                     /locus_tag="PLT86_06390"
     CDS             complement(2758..3492)
                     /locus_tag="PLT86_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOT36275.1"
                     /translation="MTEGPAGVVVIPTYNERENLERLVDEIRSLNLNLDVLVVDDNSP
                     DGTGSLAERLRSEGRVAHVIHREGKLGLGSAYVAGFRWALAKNYPLIFQMDADFSHDP
                     HALPDFMSAIEHADLVIGSRYINGITVINWPIGRLLLSYFANAYARFVTRMPLRDMTG
                     GFKCFRRDALKRINLDRISSEGYSFQIEMNFIALHLGFRVKEIPIVFRDRQAGTSKMN
                     FRINREAAWMVWKLRLLAWAGRLGHG"
     gene            complement(3507..4709)
                     /locus_tag="PLT86_06395"
     CDS             complement(3507..4709)
                     /locus_tag="PLT86_06395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT36276.1"
                     /translation="MQCMAANTPELERSYAEVAGRAFKRPGEMRDHILKWMKRPRYSH
                     AHTRIVVDNGAVVGGLAIVELPVRYGDTVLKLGGISCVATDPESQKRGYGRANMEDAV
                     AYMAQNGYDLSFLLGIRDYYYRYGYRTAIVWFECRISPNVYSAKPAPGLRVRRARKSD
                     IPGMAALYEKNGAQCDLSVVREKKDWLWYFDFGRFSDAHVVVNSEKNIVGYLVCRGAG
                     RVDEIGLEDTTEVYETVLAFLQEKAKQAFSTQVTVYTPGENGFARYCLYKKRSTWARW
                     TDYQGGPMLRLFNVENLFRKIAPTLQIRWQRVPRGLRPVAFTVQCPMGQVAFAPTETD
                     LTIKPGAVAGEVVVLPDEALTELVLGFRPAADVVADAGARPAAATVALLDAIFPVSTT
                     FLSPTDHM"
     gene            complement(4730..5266)
                     /locus_tag="PLT86_06400"
     CDS             complement(4730..5266)
                     /locus_tag="PLT86_06400"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOT36277.1"
                     /translation="MEGNAYNEAIFAPDLCSPDAPPLDPSQVRIFRDPHGYLRATIGD
                     RTYLDVTITRAFPLQASDRYICILTGRLGEVGVIENLADLDEESRRIASEELDRRYYL
                     PRIQRIKSIREEFGATYWVVETDFGERSFVGKGVRDNVHYVNSERLLIQDVDGNRYEV
                     YDVNALDPASRSLLFRVT"
     gene            complement(5358..6119)
                     /locus_tag="PLT86_06405"
     CDS             complement(5358..6119)
                     /locus_tag="PLT86_06405"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOT36278.1"
                     /translation="MQVDNSPVTVSTEKPLFFFSDAHIHTRTTRDEAEKQDALVAFLR
                     AIKAEAGGVFVVGDLFDFWFEYGAVVPRHGTRVMVALAELVDAGIPVICLGGNHDWWL
                     GNALTEEYGVYVHRCPLWVRAQEQTIFLAHGDGLSGPDRYYRLVRAVLHNRISEAAFR
                     VLHPSCGAVLANLASRTRSNREYCDTVQAQITPVFRAAVPEVFARGASIAVFGHVHTV
                     RLELVDNNVLAVLGDWINLRSYGELREGAFRIRTW"
     gene            6254..6481
                     /locus_tag="PLT86_06410"
     CDS             6254..6481
                     /locus_tag="PLT86_06410"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOT36279.1"
                     /translation="MPKTPGWADSLREVAPLLSLGIQLALIVVVSVIIGWWVDQRYGT
                     GPWGVLIGAVFGTGSGLYHFIRTALGMKDRH"
     gene            6495..6941
                     /locus_tag="PLT86_06415"
     CDS             6495..6941
                     /locus_tag="PLT86_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36280.1"
                     /translation="MTLEKETGTLLRTWALWFFGLTILSALLSAYPLLISSSLHRMSA
                     MIGWGVSFLVCVVSGYLAFWSLGRSHNTFLLVALGGIVLRLALISGAVFVGISLLHLH
                     TVIFLGSLLGSYTMYHGLEIIVLNQSKALVRTPGGSGAVARGNEAH"
     gene            6941..7945
                     /gene="atpB"
                     /locus_tag="PLT86_06420"
     CDS             6941..7945
                     /gene="atpB"
                     /locus_tag="PLT86_06420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT36281.1"
                     /translation="MVFPRVRLVAKTFVLVAILSCPALAADSHASAADTAVVGAHGQG
                     EVAAAAHGEGHGNQVDAIHHVVDAREIEIPWPNRDLAKVIHLPSLGTFHIGSIPVDMA
                     ITKHVVFLWVAAILLFVVVSVAAAKRSRGTDPPRGITNLIEIFVAFIRDEITVPSLGP
                     HYGRVLLPYFLTAFFFILFCNLLGLVPYGSTATGNISVTAALATLSFFVIQGVAISSM
                     GFGGWIKHLTGGVHWALWPIMIPVEIMGLFTKPFALCVRLFANMTAGHVVILSILGLI
                     FIFRSVAVAGLAVPFSVFIYLLELLVAFIQAYVFTMLSAVFAGLGVHSSHDSEEAEAH
                     "
     gene            8033..8260
                     /locus_tag="PLT86_06425"
     CDS             8033..8260
                     /locus_tag="PLT86_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT36282.1"
                     /translation="MEALSLAHFAAGIGAGIVTFGAAFGIGKIAQAAMDGTARQPEAG
                     GQIRISMLIAAALIEGIAMFGQVICVLLAIK"
     gene            8387..8881
                     /gene="atpF"
                     /locus_tag="PLT86_06430"
     CDS             8387..8881
                     /gene="atpF"
                     /locus_tag="PLT86_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT36283.1"
                     /translation="MLVSVNPGLIIWTIITFLVLVFLLSKFGWKPIVGALENREQAIR
                     QAIAEAHQARDEAQKILEAQNKLIASAEAEAREIVQAARETSEQIRREADVTARTESQ
                     RLLDQARREIENAKELALRDIRDVVADLAVAGAGKILKETLDPERHRAMVDDLVASLE
                     KSKA"
     gene            8921..9490
                     /gene="atpH"
                     /locus_tag="PLT86_06435"
     CDS             8921..9490
                     /gene="atpH"
                     /locus_tag="PLT86_06435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOT36284.1"
                     /translation="MAIHSRVSIRYARALVLAAQEADNIASLRQETEGLTELMHRTPS
                     LVKFLDDPSINKHDKSRILNAVFGDHLSPLMRRFVTMLVGGYRERLLPDILAAVLTLL
                     DAREGRVHAEVASATPFADAQPERLRHALEGLTGKKVTMVTREEPDLGAGFVVRVGDT
                     VFDASLRAQLAQMRAQLLQSNLRPAVRTS"
     gene            9565..11103
                     /gene="atpA"
                     /locus_tag="PLT86_06440"
     CDS             9565..11103
                     /gene="atpA"
                     /locus_tag="PLT86_06440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT36285.1"
                     /translation="MAVTFDVRPDEVSQILAQQLAGFERGVDVYEAGTVLQVGDGIAR
                     VHGLSNVMAGELVEFPHGIMGLAFNLEEDNVGCILFGETTEIKEGDTVKRTKRILQVP
                     VGEALLGRTVDALGSPLDGKGDIVTDRYDFVERKAPGVIQRQPVKTPLQTGLKAIDAM
                     TPIGRGQRELIIGDRQTGKTALAVDTIINQKGQNVFCIYVAIGQKASTVANVVGILES
                     HGAMDYTTVVSAPASTPAPLQYLAPYSGCTMGEFFRDSGRDALIIYDDLTKHAWAYRQ
                     VSLLMRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSLTALPIIETQEGDISTYI
                     PTNVISITDGQIFLESDLFYGGQRPAINPGISVSRVGSKAQIRAMADRDVAGRLRTDL
                     ARYNEVAAFAQFGSDLDAVTQQTLRRGERLQEILKQGQYVPMPVEEQVLSLFAATTGA
                     LDELPVHSVRQWEREYLENMRAAHADVLKEIREKRTIPAPLKEKIREITKQFTKTFKP
                     AGTA"
     gene            11148..12011
                     /gene="atpG"
                     /locus_tag="PLT86_06445"
     CDS             11148..12011
                     /gene="atpG"
                     /locus_tag="PLT86_06445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOT36286.1"
                     /translation="MPNLRMIRRRINAVNNIQQVTRAMKMIAASRMRKAQENILVARP
                     YVRRLETLLRHIASRIPQGAHPLLEVRPVKKALLIVVAGDRGLCRGFNANAIRRTVER
                     YGELKAQGVECSLIAVGRKPYEYFGKRGFPIAASHTGIFQHLNFLDAQEIASEATKLF
                     LNRDADLVEIVYNEFKSVIQQVVVLERFLPVEPIAPEDGDMPCHYLYEPSLEAIWSSI
                     VGKFVNLEIWRVLLESNAAEQSARMTAMEEATKSAEEMTKRLIGIRNQVRQTNITTEL
                     TEIVGAANALE"
     gene            12099..13499
                     /gene="atpD"
                     /locus_tag="PLT86_06450"
     CDS             12099..13499
                     /gene="atpD"
                     /locus_tag="PLT86_06450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT36287.1"
                     /translation="MNVGKVVQVMGAVIDVDFTGAELPAILNALEIDRENGERLVLEV
                     AQHLGENKVRTIAMDATEGLVRDMPVRDTGVPISVPVGKRTLGRMLNVIGEPIDGKGD
                     LGTGKRLPIHRPAPLLTSLTVKAEMFETGIKVIDLVQPIMRGGKVGLFGGAGVGKTVL
                     ITELIHNIAKEHGGYSVFAGVGERTREGNDLFLEMGESGVLERTALVFGQMNEPPGAR
                     ARVALTGLTLAEDLRDESGVDVLLFIDNIFRFVQANSEVSALLGRMPSAVGYQPTLGT
                     DIGELQERITSTKQGSITSMQAIYVPADDYTDPAPVATFAHLDAVTYLDRAIVEQGRY
                     PAVHPLRSTSRILDPLVVGEDHYNVARQVQQVLQRYEDLRDIIAILGMDELGDDDKMA
                     VLRARKIQMFLTQPMFVAEAFTGTPGKYVPVVETVRGCREILEGRHDDLPEEAFRMVG
                     TIDEAVERAERLRSGS"
     gene            13499..13942
                     /gene="atpC"
                     /locus_tag="PLT86_06455"
     CDS             13499..13942
                     /gene="atpC"
                     /locus_tag="PLT86_06455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOT36288.1"
                     /translation="MPGTFSLEIVTPTRILFQGEVEMLRAPGTLGSFQVFPGHAPLIS
                     TLEVGEIDIRLPGGKERAIATSGGFVEVLRKGVTVLAETAEFAEEIDKARAEQAVARA
                     KQQLEHLRTTARVKAEAATRQVSDAEVEMALIRALNRLRVAQREH"
BASE COUNT         3008 a         3998 c         4205 g         2895 t
ORIGIN      
        1 accgtaatcg cgctggatca gtctcccggt tggcgtatag tagcgcgcaa cagtaaggag
       61 aagaacgcct ccgccctggg cctgctgact caggtcgaac tgccgctgca ccaacccctt
      121 cccgaaactt tgttgtccca cgatgattcc gcgatctgta tcctgcagcg cgcccgcgac
      181 aatttctgaa gcgctcgcag atccccggtt tatgagcacg acgacaggtg tatcaacttt
      241 ccgtgaaccg gatgtggcaa aataggtctg gttcgcctgc tcccagcgcc cgcgctgaga
      301 aacaatgacg gaccctgcgt cgaggaagaa atcggcgact gcgactgcgg cgttcagttc
      361 cccacccccg ttgtccctca ggtccagaac cagacaccgc atccctgcgt cgttcagcct
      421 gccgatggcc tcttccactt cctggccggt ggtctgtgca aaattcgcga ttcggatgta
      481 accggtggtg tcatcaagca tgaaggcgta gggaacgctc ttcagcggga tcactcctcg
      541 aacgatttcc acaggcaggg gcgtttcccg cccgttccgc cggatctcga gccgcactct
      601 ggtgccaacc ggccctttca ggtgctcctg gacctcagcc tcgcgccagt tgactgttgt
      661 cagaccatct acggaaagaa tgcgatcgcc ggaaagaagc ccggcgtgtt ccgccggccc
      721 gccttcgatc acagaggtta ccgtgggaag cccttcaatg atggtgtaat agatgccgac
      781 gcctccgaaa cttccgcgta tccgttcgcc aaaacgagca gcttcttccg gaggcagata
      841 gtcgctgtga gggtcaagac gatcgagaag accgtctatt gcgccgtcca tcagtctgtc
      901 tgtgtctacc tcgtcgacgt acacatcccg ggccagtcga acgattgtac tgaggattgt
      961 cagccgcgcg tagaaaccgc gggcggccgc cattaccttc ggcgcgcaga tcgccaggaa
     1021 gacagccatc gcgccaagga tcaccgcgtt ccttgttcta ctgctgcgaa aacaggaagg
     1081 cacggacttc attgaaggaa ccaggtccgt ttctgcaggt tcaggtccat gaagcggttg
     1141 tccggcggtg tttccgcccc gacagggaca tcgtcgtccg caacaagccg gacccgtccg
     1201 ggataccgcc ccccacgcct cacgaggtcg agaagctcat ggtaatggta tttctctttc
     1261 gccacgatca agtcgtcaac acgaaagctg cgcaacagga ttggaaggtc cgatgcacta
     1321 ccgacgaccg ggaaccccgc tacggcggtg cagtcgatcg gatcatcttc gctgcgaacc
     1381 acacccagca cgtgatagcg gtcttcggat gacgaaaggg aattgagaaa gcgttgcgcc
     1441 ttttcgccac aacccagcac cagccacccg gcgcgtggcg gttgcgatgc gcgggaaaca
     1501 agccagcgcc agagcagcat cccgcccccg cccagggccc acgtaaccag tgtcacgatg
     1561 cgcgaaaagt ttatcgttcg gacaaggaaa atcgttatga ttgacgcgac ggcccctatg
     1621 gcagcagcga cagctgcgcg acgcagagcg agacgattcc ggacgtacaa accggtcgcg
     1681 gcggctccca gcaaaccgga ggcaacaaaa accatccata tcgtggccca ttgccggatg
     1741 ctgaatggtg gaatggtcca tcctgtttgt gtccttgcga acagcgcaag cacgaaaccg
     1801 gcggtaagca cggccacgtc agcgatcgga acgaccgcag cgaggagcac tctccagagt
     1861 gttatcgcgg ctccacggac aactatgcca aactcaatca gccacacgag aagaggaatc
     1921 ctcgtaggtg ctatgtgctt gcggacgaat acgtgcatcg cccggtggaa caaatagagc
     1981 tggcgcaagc gggcgatgga tcgggtgctt tcccccctga aatggacgat ctccgcagac
     2041 ggtacataac cgatcgtcca accggcagac cggatacgaa acgaccaatc caggtcttcg
     2101 ccgtacatga aaaagctctc gtcgagcagc ccgatccgct caacgacttc cctgcgaacc
     2161 gccatgaaac acccggaaac agcgtccact tcacattctt gcccgtcgtc gtgataggtc
     2221 atgttataac gggcgaaacg acgactcttt gggaaaatga agctgagacc gaccatgcga
     2281 taaaaggctg ccgagggcga agggaaaccc ctccgcgcgc cgcggtccgt ggttccgtca
     2341 gggttgacaa cgcggccccc ggctgcgcct gcggaagggt gcgtgtcaag gtacgcgacc
     2401 agggcttcga atgcgcgcgg atgaacgatt acatctggat tgagcaggag gatgtaccgc
     2461 cccctggcga tcctgattcc gagattgttg ccacccgaaa agcccaggtt ccgatcactt
     2521 cggatcagcc tgacttcccg gaaatccgcg gcaaccatgt cggcgcttcc gtcctgcgac
     2581 gcgttgtcaa ctacgattat ctcggcagca agggagcgtg ttgcctgcaa cgcggtgcgg
     2641 attgccagtg caaggtatgc gcgaacgttg tagctcaaaa tgacgacaga gaggtcaagg
     2701 gaaggagcgc tgtgcgcgtt ttcttcgata gagcgggcgt tctctccgcg cattgactca
     2761 accatgcccc agtctcccgg cccaggccag caagcgcagc ttccacacca tccatgccgc
     2821 ttcacggttg atgcggaaat tcatcttgga agtgcccgcc tgtctatcac ggaaaacaat
     2881 gggaatttct ttcacgcgga accccaagtg gagggcgatg aagttcattt caatctgaaa
     2941 gctgtaccct tccgaactga ttcgatcaag attgatccgt ttcagagcat cgcggcgaaa
     3001 gcacttgaac ccgccagtca tgtcccgcaa gggcatccgg gtaacgaacc gcgcgtacgc
     3061 attcgcgaaa tagctgagta gcaggcggcc gatcggccag ttgataactg tgatcccgtt
     3121 gatataacgt gatcctatga caaggtctgc gtgttcaata gcggacatga agtccggcaa
     3181 cgcatgggga tcgtgggaga aatccgcgtc catctgaaaa atgagcggat agtttttcgc
     3241 aagtgcccac cggaaccctg cgacgtacgc ggagccgagc ccaagcttgc cttcgcggtg
     3301 gatgacgtgg gccactctgc cttcggaacg aagccgttcc gcgaggctcc ctgttccgtc
     3361 tggcgagttg tcatctacta ccagaacgtc gagattaagg ttaagagacc gtatctcgtc
     3421 cacgaggcgt tcgaggtttt cccgttcgtt gtacgtcgga atcacgacga cgccagccgg
     3481 cccttcggtc atgcgctctc cgtgtgctac atgtggtccg ttggggaaag aaacgttgtc
     3541 gacacaggaa aaatggcgtc cagcaacgcc actgtcgcag cagccgggcg tgcacccgcg
     3601 tccgccacta catctgccgc cgggcggaag cccagaacca gttccgtgag cgcttcgtca
     3661 ggcaacacga ccacctctcc cgcgactgct ccgggtttga tcgtcagatc ggtttcggtg
     3721 ggcgcgaaag ctacctggcc catcgggcat tgaacggtga aggcaacggg ccgaagaccc
     3781 cgcgggaccc gttgccatcg gatctgaagg gtgggagcga tcttcctgaa caggttctct
     3841 acgttgaaca ggcgcagcat cgggccaccc tggtaatcgg tccaacgcgc ccacgtggac
     3901 cgcttcttgt aaaggcaata acgggcgaac ccattctccc cgggcgtgta gaccgtaacc
     3961 tgggtagaaa acgcttgctt cgccttctcc tggaggaaag ccagcaccgt ctcgtacacc
     4021 tcggttgtgt cctcaagtcc gatttcgtcg actcttcccg ctcctctgca tacgaggtac
     4081 ccgacaatat tcttctcgct gttcaccacg acgtgagcat ctgaaaaccg accaaaatcg
     4141 aagtaccaca gccagtcctt cttctcgcgt accacggaaa ggtcgcactg cgctccgttc
     4201 ttttcgtaca gggcggccat ccccggaatg tctgacttgc gtgcccttcg aacgcggaga
     4261 cccggcgcag gtttcgccga gtacacgtta ggagaaatcc ggcactcaaa ccacacaatc
     4321 gccgtacgat agccatagcg atagtagtaa tcccgaattc ccagaagaaa ggaaagatcg
     4381 tacccgttct gtgccatgta cgcgacggcg tcttccatgt tggcgcggcc gtatccgcgt
     4441 ttctgggatt ccgggtctgt cgcgacgcaa ctgatgccgc cgagtttcag cacggtatcg
     4501 ccgtaacgaa ctggaagttc tactatggcg agcccgccga caacagcgcc gttgtcgacc
     4561 acaatccggg tgtgagcgtg ggagtatctg gggcgtttca tccacttgag aatgtggtct
     4621 cgcatttccc ccgggcgttt gaaggcgcga ccggcgactt ctgcgtaact acgctccagc
     4681 tccggcgtgt tggcggccat acactgcatt gagtgctgtc tccctcggtt caggttactc
     4741 ggaaaagaag agaacgactt gccggatcaa gtgcattgac gtcatagacc tcgtaacggt
     4801 ttccgtcaac gtcctggatg agcagtcgtt cggaattgac ataatggaca ttatcgcgca
     4861 ctcctttgcc aacaaaagag cgctcgccga aatccgtttc taccacccag tacgtagcgc
     4921 cgaattcctc gcggattgat tttatccgct ggattcgagg caggtaatac cgccggtcca
     4981 gttcctcgga tgcgatccgc ctgctctctt cgtcgaggtc ggcgaggttt tcaattactc
     5041 cgacttcacc gaggcgtccg gtaagaatac agatgtaacg gtcgcttgcc tgcaatggga
     5101 atgcgcgggt gatcgtgacg tcgagatagg tgcggtcgcc aatcgtcgcc cgcaggtacc
     5161 cgtggggatc gcggaaaatt cgtacctgcg agggatcgag agggggagca tcggggctgc
     5221 aaagatccgg agcgaaaatc gcctcgttgt acgcgttgcc ttccattcag acacctctgc
     5281 gccgtgggga aacgggtata aacaaaatgt atggcttcga acgcccatcc ccaacggcgg
     5341 ggcatcaact cgttctgtca ccaggttctt atgcggaacg ctccctccct gagttccccg
     5401 taggatcgga gattgatcca atctcccagc actgccagta cgttattgtc gaccaactcc
     5461 aggcggactg tgtgcacatg gccaaaaacc gcaatgctgg ctcctcgggc aaaaacctcg
     5521 ggaacagcgg cgcggaacac aggggtgatc tgcgcctgaa ccgtatcaca gtattcacgg
     5581 ttactgcgag ttctggacgc aaggtttgcc agaaccgccc cgcatgacgg atgcagaaca
     5641 cgaaacgccg cctcagagat cctgttgtgc agcaccgcgc ggacaaggcg gtagtaccgg
     5701 tccgggccag acaagccgtc gccgtgcgcg aggaaaattg tctgttcctg cgcgcgcacc
     5761 cacaatggac acctgtgaac gtacacccca tactcttccg taagagcatt tcccagccac
     5821 cagtcgtgat ttccaccgag gcagatgacg ggaatgccgg catccaccag ctcggcaagt
     5881 gcgaccatca cacgcgttcc gtgacgaggc acgaccgctc catattcgaa ccagaaatcg
     5941 aaaaggtcgc ctaccacgaa caccccgccc gcttcggctt tgatcgcacg caggaaggca
     6001 acaagagcat cctgtttctc tgcctcatcg cgggtcgttc tcgtgtggat atgcgcgtct
     6061 gaaaagaaga aaagaggctt ttcagtggat accgtaacgg gggaattatc gacctgcacg
     6121 tctcgctggc ctccgccggg ggttgacacg gattcgacaa aaccagagtt ttgatattcg
     6181 ccctcaccta tggtgagtcc aaatctgcct gcagtcaagg caggtgtccg gggcggcgaa
     6241 cggagtgaaa tgagtgccga aaactccagg ttgggccgat tcgttacgtg aagtggcgcc
     6301 tctgctgagc ctcggaattc agctcgcgtt gatcgtggtg gtgagcgtga tcatcggctg
     6361 gtgggtggat cagcgctacg gaaccggtcc ctggggtgtc cttattggag cggtgttcgg
     6421 tactggatcc gggttgtacc atttcattcg caccgctctt ggcatgaagg acaggcactg
     6481 agaccgtccg aagaatgacc cttgaaaaag aaacggggac cctcctgaga acctgggcgc
     6541 tgtggttttt cggtttgacc attctctcgg ctctgttgtc tgcctacccg ttgctcatca
     6601 gttcttcgct ccaccggatg agtgcgatga tcggttgggg cgtcagtttc ctcgtgtgtg
     6661 ttgtgagcgg atatcttgcg ttctggtcgc tcggccgctc gcataacacg tttttgcttg
     6721 tagcgctcgg cggaatagtg ctgcgccttg cgctgatttc cggggcggtg tttgtgggga
     6781 tctctcttct gcacctgcac accgtgatct ttctcggaag cctgctgggt tcctacacga
     6841 tgtatcacgg gttggaaata atcgtgctta accaatccaa ggcgctggtc agaacacctg
     6901 gcggctcagg tgcggttgca cgaggtaatg aggcgcactg atggtgtttc cgcgcgttcg
     6961 cctggtggcg aaaacctttg ttctggtggc gattctttcc tgcccggcgc ttgcggcaga
     7021 ttcgcatgca tcggcggcgg acacggctgt cgttggcgcg cacggtcagg gcgaagtcgc
     7081 cgcggcagct cacggcgaag gacacggcaa ccaggttgac gcgatacatc acgtggtgga
     7141 tgcccgtgag atcgagattc cgtggcccaa ccgagatctg gcaaaggtaa tccatctgcc
     7201 cagcctgggc acgttccata tcgggtccat tcctgtggac atggcgatca cgaaacacgt
     7261 cgtgttcctc tgggtggcgg cgatactgct gtttgttgtt gtgtcggtgg cggcggcgaa
     7321 gagatcgcgt ggaactgacc caccccgggg cattacgaac ctcatcgaaa tctttgtcgc
     7381 attcattcgc gatgagatca ccgttccgtc ccttgggcca cattacgggc gggttctgct
     7441 tccgtatttt ctcaccgcgt ttttcttcat tctgttctgc aatttgctgg ggttggttcc
     7501 atacggttcg accgcaacgg gtaacatcag cgttacggcg gcgctcgcca cactgagttt
     7561 cttcgtaatt cagggcgtcg cgatcagcag tatggggttt gggggctgga tcaagcatct
     7621 gacgggcgga gtgcactggg cgctgtggcc catcatgatc ccggtggaaa tcatggggtt
     7681 gttcacgaag cctttcgcgc tgtgtgttcg tctttttgcc aatatgactg ccgggcacgt
     7741 ggtaatcctg tctattctcg gactgatttt cattttccga agcgttgcgg tggctggtct
     7801 cgcggttccg ttcagcgtgt tcatctacct gctcgagctt ctggtggcct tcatacaggc
     7861 gtacgtgttc acgatgttga gtgccgtctt cgcgggtctt ggcgtgcaca gcagccatga
     7921 ttcagaagaa gcggaggcgc attgatttgt cggcggcatc cgatctgccg atgcgttgat
     7981 tacccatagc caacgccgca ctgcggcgtg atctggttca ggaggagttt tgatggaagc
     8041 tctcagtctg gctcactttg ccgccggtat cggtgctgga atcgtcacgt tcggggccgc
     8101 gttcggaatc ggaaaaatcg ctcaggcagc gatggacggt accgcgcggc aaccggaagc
     8161 gggtggacag atccgtatct ccatgctgat agccgccgcc ctcattgaag gtattgccat
     8221 gttcgggcag gttatctgcg ttctgctggc aatcaagtga tttcaaaccc cggcgtcacg
     8281 tgtaccaccc gaggcagggc gagggaagac acgtttccgc gccgttctgc ttccggtgat
     8341 gtgaacgtgc gcggggtgca ggcatcactt tttcccgggg atatccatgc tggtaagtgt
     8401 taatcctggg ctcatcatct ggacgatcat cacgttcctg gtgctcgttt ttctcctgag
     8461 caaattcgga tggaaaccca tcgtcggcgc tctggaaaac cgggaacagg cgattcgaca
     8521 ggcgattgcc gaagctcatc aggcacgcga tgaagcgcag aaaatcctgg aggctcagaa
     8581 caagctgatc gccagcgctg aggctgaagc ccgggagatc gttcaggcag cgcgggaaac
     8641 gtcagagcag attcggcggg aagccgacgt gactgctcga acggaatccc agcgattgct
     8701 ggaccaagcc cgtcgcgaga tcgaaaacgc gaaggaactg gccctgcgcg acatacgaga
     8761 cgtagtcgcc gaccttgcag tggcaggtgc cggcaagatc ttgaaggaaa cgcttgatcc
     8821 ggaacgtcac cgtgcgatgg ttgatgatct ggttgcaagc cttgaaaaat cgaaggcgtg
     8881 aacgttaccg cattcgttcc gccggtaacc agagaaactg atggcaattc attctcgcgt
     8941 gagcattcgc tacgcccggg cactcgtcct cgcagcgcag gaagccgaca acattgcctc
     9001 cctgcgtcag gagactgagg ggctgacgga gttgatgcac cggacgccgt cgctggtgaa
     9061 attcctcgac gacccgtcca tcaacaaaca tgacaagtcg cggatactga acgcagtgtt
     9121 cggagaccac ctctcgccac tcatgcggcg cttcgttacg atgctggtgg gtggttatcg
     9181 cgaacgtttg ttgcccgaca tcctcgcggc ggtcctgacg ctgctggacg cgcgcgaagg
     9241 aagggttcat gcggaagtgg cttctgcgac gcctttcgcg gacgcacagc ctgagcgcct
     9301 acgtcacgca ctggaaggcc tcactggcaa gaaggtgacg atggtgactc gggaggaacc
     9361 ggatctcggc gccgggttcg tggttcgcgt cggggatacg gtgtttgacg cgtctttgag
     9421 agcgcaactc gcccagatgc gcgctcaact tctgcagagc aatctgcgtc cggcggttcg
     9481 cacttcttga gtgttgcgag cggagactga cacagtgcaa agagagcaat ttcggttaca
     9541 gcaggggaaa gaaaaggaaa cacgatggcg gtaaccttcg acgtgcgtcc ggatgaagtg
     9601 tcccagatac tggcgcagca acttgccgga tttgaacgcg gtgttgacgt gtacgaggcg
     9661 ggaactgtgc tgcaggttgg ggacggtatc gcccgcgttc acgggctttc caacgtaatg
     9721 gccggagaac tggtggagtt cccccacgga atcatgggtc tcgcgttcaa tctggaggaa
     9781 gacaacgtcg ggtgcattct tttcggtgaa accactgaaa tcaaggaagg cgacacggta
     9841 aagcggacga agcgtattct gcaggttccg gtgggggaag cccttctggg gcggaccgtt
     9901 gatgcgcttg ggagcccgct tgacgggaaa ggcgacatcg tcaccgaccg gtatgatttc
     9961 gtcgaacgga aggctccggg agtcattcaa cgtcagccgg tcaaaacgcc gctccagaca
    10021 gggctcaagg cgatcgatgc gatgacgccg attggccgcg gacagcggga gctcatcatc
    10081 ggtgaccggc agacggggaa aacggccctg gcggtagaca cgatcatcaa tcaaaagggg
    10141 cagaacgttt tctgcatcta cgtcgccatc ggccagaagg cctcaacggt ggccaacgtt
    10201 gtgggaatcc tcgaatctca cggcgccatg gattacacca cggtagtttc tgcgcccgcc
    10261 agcactccgg ctccgctgca gtacctcgcc ccgtactccg gctgcaccat gggagagttt
    10321 ttccgtgatt cagggcgcga cgcgctgatc atatatgacg atctgacgaa gcacgcatgg
    10381 gcataccgtc aggtgtccct gttgatgcgc cgcccgccag gccgcgaagc ctatccgggt
    10441 gatgtgtttt atctccattc ccgattgctc gaacgtgcgg cgaaactctc cgacgacctg
    10501 ggtggcgggt cgcttaccgc actaccgatc atcgagactc aggaaggcga catctccacg
    10561 tacatcccga caaacgtgat ttccatcacc gacgggcaga ttttcctcga aagcgatctt
    10621 ttttacggcg ggcagcgccc ggcgatcaac ccggggattt cggtgtcgcg cgtcggtagc
    10681 aaggcgcaga ttcgtgccat ggcagataga gacgtggcgg gacgtcttcg cacagacctg
    10741 gcgcggtaca atgaagtcgc ggcgttcgct cagttcgggt ctgatctcga cgcggtcacg
    10801 caacagacgc tgaggcgtgg tgagcggctg caggagattc tgaaacaggg tcagtacgta
    10861 ccgatgcctg tcgaagagca ggttctttcg cttttcgccg ctactacggg agcactggac
    10921 gaactgcctg tgcattcggt gagacagtgg gagcgcgaat acctggaaaa catgcgagcc
    10981 gcacacgcgg acgttctgaa ggaaatcagg gaaaagcgaa caatcccggc acctttgaag
    11041 gaaaagatcc gggaaattac gaagcaattc acaaagacgt tcaaaccggc gggaaccgcg
    11101 tgaaacggtc agcagacccg cgataagtat cggatagaga ggtccccatg cccaatctgc
    11161 ggatgatacg gcgtcgaatc aacgccgtga acaacatcca gcaggtcacc cgcgcgatga
    11221 agatgatcgc ggcctcccgc atgcggaagg cgcaggagaa cattctcgtg gcgcgccctt
    11281 atgtgcgccg cctggagact ctgcttcgcc acatagcctc ccgtattcct cagggagcgc
    11341 acccacttct ggaagtgaga cctgtcaaga aggcgctgct gatcgttgtc gcaggcgatc
    11401 gaggtctctg ccgggggttc aacgcaaatg cgattcgtcg tacggtggag cgctatgggg
    11461 agctgaaagc gcagggtgtg gagtgttcgt tgatcgcggt cggacgaaag ccctatgaat
    11521 acttcgggaa acgcggtttc ccgatagccg cctcacatac aggaatcttc caacacctga
    11581 atttccttga cgcgcaggaa atcgcgtctg aagcgacaaa gctcttcctg aaccgtgacg
    11641 cagacctcgt ggagatagtg tacaacgagt tcaaatcggt gatccagcag gtggtggttc
    11701 tcgagcggtt tcttccggtg gagcccatcg ccccggagga cggggatatg ccatgccact
    11761 acctgtatga gccctcactg gaagcaatct ggagttccat tgtcgggaaa ttcgtcaatc
    11821 tggaaatctg gcgtgtgttg cttgagtcga atgcggcgga gcagtcggcc cggatgaccg
    11881 cgatggaaga ggcgacgaaa agcgcggaag agatgacgaa acgactgatc ggtatcagaa
    11941 atcaggtccg acagaccaac attacgacgg agttgactga aattgtcggt gcggccaacg
    12001 cactggagta acgtgtttga gagtgttgtg caggcacgtg ggtgccgtgc cgaacatccg
    12061 gcagggaaac gttttcacaa gctacgggaa acccgctgat gaatgtaggt aaagtagttc
    12121 aagtcatggg cgcggtcata gacgttgact tcactggcgc tgaattgccc gcgatcctga
    12181 atgcgctcga aatcgataga gaaaatggcg agcggctggt tctggaggtt gcgcagcacc
    12241 tcggagagaa caaggtaagg acgatcgcca tggacgcgac cgagggactg gtgcgcgata
    12301 tgcccgtgcg ggatacgggt gtcccgattt ctgttccggt cggcaagaga acgttgggga
    12361 ggatgctgaa cgttatcggc gagcccatcg acggaaaagg tgaccttgga acggggaaac
    12421 gtctgccgat tcaccggcca gcgccactcc tcaccagcct gacggtcaaa gccgaaatgt
    12481 tcgagactgg catcaaggtg atagaccttg tgcagccgat catgcgcgga ggcaaggtcg
    12541 ggttgttcgg cggcgcaggt gttggcaaga cagttctgat tacagagctc atccacaaca
    12601 tcgccaagga gcacggtgga tactcggttt tcgccggggt tggcgaacgg acccgagagg
    12661 gcaacgacct gttccttgag atgggggaat ccggggtact ggagcgcact gctctggttt
    12721 tcggccagat gaacgaaccg cccggggctc gtgctcgcgt cgcattgacg ggactcaccc
    12781 ttgcggaaga cctgcgggat gagtccggtg tggacgtctt gttgttcatt gacaatatct
    12841 tccgttttgt gcaggcgaac tcagaagtgt cggctcttct gggcaggatg ccctccgccg
    12901 tggggtacca accgactctg ggaacggaca tcggagaact ccaggaacgc atcacctcaa
    12961 caaaacaggg ctccatcacg tcgatgcagg cgatctacgt tcccgccgat gactatacgg
    13021 atcccgcacc ggtggccacc ttcgcccatc tcgacgcggt aacctacctt gaccgcgcga
    13081 tcgtggaaca gggccgctat ccggccgttc atccgctacg gtcaacgtcg cgaattctgg
    13141 acccgctcgt cgtcggcgag gaccattaca acgttgctcg ccaggtgcaa caggttcttc
    13201 agcgctacga agaccttcgc gacatcatcg ctatcctggg catggatgag ctgggcgacg
    13261 acgacaaaat ggcagttctt cgcgcgcgga aaattcaaat gtttcttacg caaccgatgt
    13321 ttgttgccga ggcgttcacg ggtaccccgg gaaaatacgt tccagtggtg gaaacggtgc
    13381 ggggatgccg tgagatactt gagggcagac acgatgatct tcccgaagag gccttccgca
    13441 tggtaggaac gatagatgag gccgtggaac gagctgaacg gctcaggagt ggttcatagt
    13501 gccgggaacg ttttccctcg aaatcgttac ccctacgcgg attctctttc agggcgaagt
    13561 ggaaatgctt cgcgcgccgg gaactctggg gtcgttccag gtttttccgg gacatgctcc
    13621 cctgatttcc actcttgagg tcggcgagat tgacatccgt ctgcccggtg gcaaggaacg
    13681 agccattgct acttcgggag ggtttgtcga agtccttcgg aagggtgtaa cggttcttgc
    13741 tgaaaccgcg gaattcgccg aggagatcga caaagcccgc gcagaacagg ccgttgcccg
    13801 cgcgaaacag caacttgagc atctgcgcac gaccgcacgg gtgaaggccg aagcggcgac
    13861 acgccaggtg agcgacgcgg aagtggaaat ggcgctcatc agggcgttga accggttgcg
    13921 tgttgcgcaa cgggaacact gatacgaagg gcgctctgga gaggagaaca gagtgcatca
    13981 tccccgcctc cgatgatcct cgcgcacaag gcagtttcgc agtgacagca ctccccagag
    14041 acccgacaca caacgggcga aggccgcacc cccatggctc ctgacatgaa cgagatctcc
    14101 gatgag
//
LOCUS       DAOSOO010000066        14032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4368_length_14032_cov_5.601989, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000066 DAOSOO010000000
VERSION     DAOSOO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14032
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4368_length_14032_cov_5.601989"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLT86_06460"
     CDS             complement(<1..216)
                     /locus_tag="PLT86_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT36289.1"
                     /translation="MPDHPNIFVIVSDTFRPDHIGINGAGFAETPELDDFLRRGVTFD
                     NALVSSFPTIPMRTDWWTGRFGHPRYGW"
     gene            complement(514..1926)
                     /locus_tag="PLT86_06465"
     CDS             complement(514..1926)
                     /locus_tag="PLT86_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT36290.1"
                     /translation="MGTQKRTSRILRTLAIHAVLVAGGVVFSLPFFWLLTTSFKADRE
                     MFVLPPEWIPEIPPKVQYSPYQLTWRGQTTEQFFGLGPITVQDKDLSDHPVANSVSLW
                     REARGAGFAPLDSFESRPLRRVAYDFRQRDAVAYEALFPSPIPVETLRRVEISYKSDR
                     SFHRLRVQVFTPEGIWVSADPSVLNSETWKSAPWQFAKRREEERDVLRLKQLVAAPQH
                     WLVGKNGEERILVRATLERVSWLGAVWTKYTENYVDSLAYIPFGRFVVNTIILTALNI
                     VLQLLSCSLIAYGFSRIRWPGRNVMFGLLLATMMIPGQVVMIPQFMIWKSMGMYNTWG
                     PLFLGSLFGSGFFIFLLRQFMLTIPNDLEDAAKIDGAGYLAVWRMVMLPLIKPALAAI
                     AIFQFMGSWNDFFTPFLYITSKELMPISLGLYMFKDARGAEYGMLMAASTMMVVPIIA
                     LFFFAQRYFIQGVTLTGLKG"
     gene            complement(1930..2292)
                     /locus_tag="PLT86_06470"
     CDS             complement(1930..2292)
                     /locus_tag="PLT86_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT36291.1"
                     /translation="MTPERDFTDFLLDIRDNALSAMSFVRDMSFEEFEQDLRTQYAVI
                     RALEIIGEATKQVPAVVRRRHRDIPWKEMAGMRDRLVHGYSRVDLRIIWQTAKHDLPV
                     LQSVFQRLIEKESGLRGR"
     gene            complement(2289..2597)
                     /locus_tag="PLT86_06475"
     CDS             complement(2289..2597)
                     /locus_tag="PLT86_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT36292.1"
                     /translation="MEAVPTKSLSEITERLRREMPALKNQYRVRSLSVFGSYARGRQT
                     RRSDVDVLVEFEEPPGLLEFVRLQNHLRDTIGVKVDLVSKGALRGKIGAHILSETVPI
                     "
     gene            complement(2601..5207)
                     /locus_tag="PLT86_06480"
     CDS             complement(2601..5207)
                     /locus_tag="PLT86_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT36293.1"
                     /translation="MVYCFRALLICLLFSTSWASSGDTTLVVWGIGEGEGQVGFFAAV
                     EAFERANPGVRVSTSPSGGGQNPQKMLTAIVGGAAPDVMNQDRFTVSSWAARDAFLPL
                     DGRLAAQDTTAPDAIRKSEYYTACWNETVFRGKVYAIPNGTDARALYYNFAEFRRVGL
                     VDSAGVPTPPRTWRELGLFSLRLAVNDALENAGLLDADGNLIPPATWRDVEVFNLLLG
                     MPDAEARARMLDDPRHLVLPPEWTSGMVNSYRFPVTQKGLESGIINPEGKMKPPAEWK
                     FRRIGFIPVYGNVWLHMYAWQNHGKFMSPDGRTCTLNDPRIVEALEWMAWFYDMFGGR
                     PRVEAFSSSFQGQFLDPFLTGFLAMKVETDGMLGAIARYKPDLEFGVCLTPLPEGGKP
                     TTWSGGFSWAIPRNARHPDIAWRFIRHMVNYETVRLVERTRMNFNRSRGRPYVMGIPA
                     NRETGRKLAEEFLDANPDLPQRLKDGYSQYFAMMEFSDFRDVTPASQLLWDEIQRATN
                     MATYRKLTPKEAADAGAATVQQELDRLFSSSAGEPVNWTAVALVVLLLVAGGGAAGYF
                     YSREKLHLGRMGRADLVAGLGFISPWLAGFFIFTVGPFIISIVLSFTEYNMLSPARWV
                     GLKHYSELFTNDPIFWKSLGNTIYMMIGVPLGMAVGLGVAMLLNSNVRGMSWYRTVYY
                     LPAIVPTVASSILWIWVLHPTNGILNIILEYVGLGQPLWLQSEHWSKPAIIVMGLWGA
                     GSSMIIWLAGLKGIPDHLYEAAQIDGAGKWRQFVHVTLPMLSPYIFFNLIMGVIGTLQ
                     IFAQAFIMTSGGPVDSTLFYVYYLFNNAFQYLRMGYASAMAWILFVIILGLTLVQLKL
                     APRWVHYEGGGK"
     gene            5297..6154
                     /gene="folP"
                     /locus_tag="PLT86_06485"
     CDS             5297..6154
                     /gene="folP"
                     /locus_tag="PLT86_06485"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT36294.1"
                     /translation="MKEPFDSLLHPGRTLVMGVVNVTPDSFSDGGRFLSPEAAIAHAC
                     RLMDEGADLLDIGGESTAPDSPPVDAQEECRRILPVLEECAKRGFPVSVDTYKSPVAR
                     CALAHGAGMINDVTALRGDPTLADVLADSDCAVVLMFAKDPTPRTTPTVRVYADVVRE
                     ISEFLAERVAYAGTRGIAASRIVLDPGMGAFVSADPGPSLEILRRAGEFHTLGHPLLI
                     GASRKSFIGKVLGGPGQPLPVGDRLEGSLACAAIAAWHRVQIIRAHDVRETVRTVRMV
                     DAIRVADAV"
     gene            6337..6597
                     /locus_tag="PLT86_06490"
     CDS             6337..6597
                     /locus_tag="PLT86_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HOT36295.1"
                     /translation="MPELSRFFGITVRMYYNDHEPPHFHVFYGDFEALIEVGSLHVFR
                     GSLPRRAFAMVVEWAIIHREELVGAWQSARRGLAVPPIVPLD"
     gene            6609..6905
                     /locus_tag="PLT86_06495"
     CDS             6609..6905
                     /locus_tag="PLT86_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOT36296.1"
                     /translation="MSMQRIKAVEPLDGFKLKLTLTDGSVIERDVSRLLVGKVFEPLL
                     ASRTEFCRVRAEAGTVVWENGADLCPDVLIWGGPPPQDERPGIANPPVSELKPA"
     gene            complement(7047..8054)
                     /locus_tag="PLT86_06500"
     CDS             complement(7047..8054)
                     /locus_tag="PLT86_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36297.1"
                     /translation="MTGKIRWGIMSTGKIAHKFAQGLKELPDEAEILAVGSRAQQTAD
                     AFGDEFGIPRRYASYEALVADADVDVVYIGTPHSFHYDNAVLCLNAGKAVLCEKAFTI
                     TGREAEELIALARSKRLFLMEAMWVRFLPATIRVRELVSGGAIGEPRLIQASLGFWTD
                     MTPETKLSRFLNPALGGGSMFDVGIYPMSYISMLFGQARRVTGVAHIGETGVDEQASV
                     CYGYDDGRIASAVFGVRTRVPGGAFIAGSAGTLTMEPSFLNPKTLVLTEAGKDPVRID
                     VACEGTPYRFEAQEVMRCLREGKTESAVMPLDESLAIMRTMDALRRDWGMVCPNDARW
                     G"
     gene            complement(8076..9665)
                     /locus_tag="PLT86_06505"
     CDS             complement(8076..9665)
                     /locus_tag="PLT86_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36298.1"
                     /translation="MQGIMYLDALASFGKMGPKDEEVSWKLETLLDEMAWCGIHGALV
                     YHNVAREYDPAYGNRMLMEAISGNPRLFPCWVVLPHDCGEMPPPKRLVKEMLAAGVRA
                     VRMAPRSQNYLLEEYVCGELLDALAGEQILLSIGIDQTSYGDLFQIASRRPGLPILMT
                     GCSWASTRNILPLMDKCPNVHIEFSTFQANYALEEFTERYGAERLLFGTDALLKSMGA
                     AKSFVDYAELGETDRKLIAGGNLARLLRLERLPEEYPARTDGPILSRVKEGLPLEHIT
                     VIDSHAHMSHNDARGVGYMPMPRSDAKHMIRRNRRLGIDKVAISSWMGIFADCEEGNV
                     ILKDAMDQFPDDIIGYATLDPNYLTAEEFDRWMRLAHEEWGFKGMKPYNPRVRIPYNS
                     PRYDQWYAYGNEHRLFCLLHSSGGNFINEVADISKRYPEISFLLAHSGGDYPTARERL
                     PLVKERENVYLEITLTPVTYRIIEYMVKHVGAEKVLFGTDAPMRDPIPQFGWMCYSRC
                     SEEELELMLGKNMERILARCR"
     gene            9949..10025
                     /locus_tag="PLT86_06510"
     tRNA            9949..10025
                     /locus_tag="PLT86_06510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9983..9985,aa:Arg,seq:cct)
     gene            complement(10041..10970)
                     /locus_tag="PLT86_06515"
     CDS             complement(10041..10970)
                     /locus_tag="PLT86_06515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT36299.1"
                     /translation="MRHPGLYIRPLEPRDIVPVAQLLDDSLPHDRVTPRWLKEKAFAD
                     PDYDSALTLCAIDGGNLVGFAHGVARPSHAPVRGWFKWFAVAETHRRTGIATKLFDQI
                     ERVMARRGVRSVSIADSVPNYTMPGVDPRYAEARVFLAQRGYVEGEQRVNMACPLTTA
                     DWRTDEEEDRLAAQGVRVIRAARTDLSHALEFVRTQFPSWTPEVAHCFLREPISLHLA
                     VEAAGVVGFAAYDANNPGMAWFGPMGTHPEKRTRGIGGTLLKRCLRDLTAQGHAEAII
                     PWVGPVVFYEKTCGAAISRTFVAVSKQLVAERR"
     gene            complement(11059..11784)
                     /locus_tag="PLT86_06520"
     CDS             complement(11059..11784)
                     /locus_tag="PLT86_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36300.1"
                     /translation="MRKALIFLAIAIAGALAVAGTQSVRRLFSVARTFREVTFERAHA
                     DTLSLPGLAASVVGQKVLADLGLDSVQQEHIARLYRRLRESTPGDSAAPVGYSPARRR
                     DPAPAAADALRDSIRAVLTREQWKKLQDSRSTVATLRKNWTDYLSDPMVRDVMGQLDL
                     TASQKAQMKSLAKVYTGDLATLLTISDPVVRRMRVEALFDDGWSQARRILTPDQQATV
                     ENYLATHREQIEAGLRGLWLQGL"
     gene            11922..12860
                     /locus_tag="PLT86_06525"
     CDS             11922..12860
                     /locus_tag="PLT86_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT36301.1"
                     /translation="MPVSKSSSLTTSERMREEAASITRVTNIGIVMNVIMTLAKILAG
                     WAFGSIALVADGVHSLSDLATDALVIVGTRVGLRPADKRHPYGHGKIETISALLIAFA
                     LVFVGARISFVAGREMYLRHHSYPGIWVLVIAAISVVGKEWLFRITRRVALACNSAAV
                     FANAWHHRSDALGALAVVFGSVAGMMGWGHGDQTAGVVVGIMVVGVGLKIGVDALVEL
                     FDVSADVETVAALEQAISGEEGVRAFHRLRSRLVGREIFVDVHILVDPALSVVQGHRV
                     ADRVEEAIRSSMKRPANIVVHIEPDLPELRTVNPNK"
     gene            13041..13799
                     /locus_tag="PLT86_06530"
     CDS             13041..13799
                     /locus_tag="PLT86_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT36302.1"
                     /translation="MAKIPIAIQMYTLREDCSKDFVGTLKAVAKIGYAGVELAGMYGV
                     APKELGKILDDLGLKRAGGHWGVEPLEKEFDKTVEDVKALGSSFVTIPGLPGTYTEDA
                     EAWKRTAVLLSGIGQKLLKEGIQLSYHNHNHEFKLFGGKHGLDILYENSDPKALHAEI
                     DTYWVQYAGVDPATYMKKVANRLSLVHIKDMADDDMKSFAEIGNGILDWDAIFAVAKE
                     AGSKWLIVEQDSCKRPPLESAKISFENLKTMGAV"
     gene            13872..13947
                     /locus_tag="PLT86_06535"
     tRNA            13872..13947
                     /locus_tag="PLT86_06535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13905..13907,aa:Ala,seq:ggc)
BASE COUNT         2715 a         4461 c         4147 g         2709 t
ORIGIN      
        1 ccagccgtac cgcggatgcc cgaaccgccc ggtccaccag tcggtgcgca tgggaatggt
       61 ggggaagctt gacaccagcg cgttgtcgaa cgtcaccccg cggcggagga agtcgtccag
      121 ctccggcgtc tccgcgaacc cggcaccgtt gatgccgatg tggtcgggcc ggaatgtgtc
      181 cgagacgata acgaagatgt tcgggtggtc aggcatggta tctcccctcg gtggaaagaa
      241 caatcgaggt gctggtttgc agcgcgttct ggcgcgtata atgaatcccc atggcgatgc
      301 agttgccgca tccggtccac attccggccc gcgattgctg gattcccgcc tgcgcgggaa
      361 tgacgagttg ctcgatattc tcggggccac acggtctaca cggtgtatgc cctccccgtc
      421 ttcgcgaact cctcacaggt agatgccctg cgcggaaggg tagtgccgca gcaccgtgtc
      481 tcggatgatc ttttccgatt ccactcccaa ccctcaccct ttgagcccgg tcagcgtcac
      541 gccctggatg aagtagcgct gcgcaaagaa gaacagcgcg atgatcggaa ccaccatcat
      601 cgtggaggcg gccatcaaca tgccgtattc ggcgccccgg gcatccttga acatgtagag
      661 gcccaggctg atcggcatca gctccttcga cgtgatatag aggaacggcg tgaagaagtc
      721 gttccacgag cccatgaact ggaagatcgc aatcgccgcc agcgccggct ttatgagcgg
      781 gagcatcacc atccgccaca cagccaaata gccggctccg tcgatcttcg ccgcatcttc
      841 aaggtcattc ggaatcgtca gcatgaactg gcgcagaagg aatatgaaga accccgaacc
      901 gaacaacgat ccgagaaaga gcggccccca ggtgttgtac atccccatgc tcttccagat
      961 catgaactga gggatcatca ccacctgccc cggaatcatc atcgtggcga gcaggagacc
     1021 gaacatcaca tttcgccccg gccaccggat gcggctgaac ccgtacgcga tgagcgagca
     1081 gctcaggagc tggagaacga tgttgagcgc cgtgaggatg atcgtgttca ccacgaaccg
     1141 cccgaacggg atgtacgcca gcgaatccac gtagttttcc gtgtatttcg tccagaccgc
     1201 gccgagccac gatacccgct ccaacgtcgc gcgcacaaga attcgctcct ccccgttctt
     1261 cccgaccaac cagtgctgcg gagcggcaac cagttgcttc agacgcagca catcccgttc
     1321 ctcttccctc cgttttgcga attgccacgg cgcacttttc caggtctcgg aattcagcac
     1381 ggaagggtcg gcggaaaccc agataccctc cggggtgaac acctgcacgc gcagccggtg
     1441 gaacgatcgg tctgatttgt aactgatttc cacgcggcgc agggtttcca ccggaatggg
     1501 cgacggaaaa agcgcttcgt acgccacggc atcccgctgg cgaaagtcgt acgcaacacg
     1561 ccgcaacggg cgcgattcga aggaatcgag cggcgcgaaa ccggcaccac gggcttccct
     1621 ccacaggctc accgagttgg cgaccgggtg gtcggaaagg tccttgtcct gcacggtaat
     1681 cggaccgagc ccgaagaact gctcggtcgt ctgtccgcgc caggtgagct ggtacgggga
     1741 atactggacc tttggcggga tctcaggaat ccattccggc gggagtacga acatctccct
     1801 gtcggctttg aagcttgtcg tcagaagcca gaaaaacggc aggctgaaca ccaccccgcc
     1861 ggcgacaagc accgcgtgaa ttgcaagcgt gcggagtatt cgagatgtgc gtttttgggt
     1921 tcccatcgct tatcgcccgc gcaatcccga ctctttctcg atgagtcgtt ggaaaacgga
     1981 ttggagtacc ggaagatcgt gtttcgccgt ctgccatatt atccgaaggt caaccctgga
     2041 atagccatgc acgaggcgat ctcgcatgcc cgccatttct ttccatggaa tgtcgcggtg
     2101 cctgcgtctt acaactgcgg gaacctgctt cgtggcttct ccgatgattt ccagagcacg
     2161 aatcacggcg tattgggtgc gaagatcctg ctcgaattcc tcgaaagaca tgtcacgcac
     2221 gaaggacata gcactgaggg cattgtcgcg aatatcaagc agaaaatcag tgaaatcccg
     2281 ttccggcgtc atatcggtac cgtctcgctc agaatgtgcg cgccgatttt tcctctcagg
     2341 gcccctttcg aaaccaggtc cactttcacc ccgatggtgt cgcgcaaatg gttctgcagc
     2401 cgcacgaatt caagaagccc cggaggttcc tcgaactcca ccagaacgtc cacgtcactc
     2461 cgcctggttt gtctcccccg cgcgtacgag ccgaacaccg aaagagaacg cactctgtac
     2521 tgatttttga gcgcgggcat ctctcgccgc agacgttctg tgatctcgga aagtgatttc
     2581 gttgggacag cttccacgtc ctacttccct ccgccctcat aatgtaccca cctcggcgcg
     2641 agcttgagtt gcaccagcgt cagccccaga atgattacga acagaatcca cgccatcgcc
     2701 gaagcgtacc ccatgcgcag gtactggaac gcgttgttga acaggtagta cacgtaaaaa
     2761 agcgtgctgt ccaccggacc gccggaggtc atgatgaacg cctgcgcgaa gatctggagc
     2821 gtaccgataa cgcccatgat caggttgaag aagatgtagg ggctcaacat gggcagggtg
     2881 acgtgcacga actgcctcca cttccccgcg ccgtcgatct gcgccgcttc gtaaaggtgg
     2941 tccggaatcc ccttcaaccc cgccagccag attatcatgc ttgatccggc accccacagg
     3001 cccatcacga taatggcggg ctttgaccag tgctcgcttt gcagccagag cggctggccc
     3061 agcccgacgt attccaggat gatgttgagg atcccgttcg tcggatgaag cacccagatc
     3121 cagaggatgg aagacgcaac cgtcggcacg atggcgggca gatagtacac cgtgcggtac
     3181 cacgacattc cgcggacatt gctgttcaac agcatcgcca cgccgagtcc aaccgccatt
     3241 ccgagcggca cgccaatcat catataaatc gtgttcccga gcgatttcca gaagatcggg
     3301 tcattggtga acagttcgct gtagtgcttc agacccaccc agcgggccgg gctcaacatg
     3361 ttgtattccg tgaagctgag cacgatggaa atgatgaacg gccccaccgt gaatatgaaa
     3421 aaccccgcca gccacggact gatgaaaccc agccccgcca cgaggtcggc acgccccatg
     3481 cggccgagat ggagcttttc gcggctgtag aagtaaccgg cagcgcctcc gcccgcaacc
     3541 agcaacagca ccaccagcgc cacagcggtc cagtttacgg gctcacccgc cgacgaactg
     3601 aacaaccggt cgagttcctg ttgcaccgtg gcggcgccgg cgtccgcggc ttctttcggc
     3661 gtgagtttgc ggtacgtcgc catgttggtc gcgcgctgaa tctcatccca gagcaactgg
     3721 gaagccggtg tcacgtcacg aaaatcagag aactccatca tcgcgaaata ctgggagtac
     3781 ccgtccttga gccgttgcgg caggtcgggg ttcgcgtcga ggaactcttc cgcaagcttt
     3841 cggccggtct ccctgttcgc cgggatgccc atcacatagg gcctcccgcg gctgcggttg
     3901 aaattcatcc gcgtccgctc aaccagtcgc acggtctcgt agttgaccat gtgccggatg
     3961 aaccgccacg cgatgtccgg gtgccgggcg ttccggggaa tcgcccacga gaacccgccg
     4021 gaccaggtgg ttggtttgcc accttcggga agaggagtca ggcagacacc gaactcgagg
     4081 tcgggcttgt agcgggcaat cgcgcccagc atcccgtccg tttccacctt catcgcgaga
     4141 aatccggtga gaaacggatc gagaaactgg ccttgaaaac tcgagctgaa tgcttccacc
     4201 cggggacggc ctccgaacat gtcgtagaac caggccatcc actccagcgc ctcgacgatg
     4261 cgcggatcgt tcagcgtgca agtccgtcca tccgggctca tgaacttccc gtggttctgc
     4321 cacgcgtaca tgtgcagcca cacattgccg tacacaggga tgaagccaat ccgccggaac
     4381 ttccactccg ccggcggctt catcttccct tcggggttga tgatccccga ttcgagcccc
     4441 ttctgcgtaa cgggaaagcg atatgagttc accatcccgc ttgtccattc cggtggcagc
     4501 acgagatgcc gcggatcgtc aagcatgcgc gcacgggcct ccgcgtcggg catgcccagc
     4561 agcaggttga acacctccac gtcgcgccat gtagccggcg gaatcagatt cccgtccgcg
     4621 tcgagcaacc cggcgttctc cagcgcatcg ttcaccgcaa gccgcaggct gaaaagcccc
     4681 agttcccgcc acgtgcgcgg gggcgtcggc acgcccgcgg aatccaccag ccctacgcgc
     4741 cggaactccg cgaagttgta gtagagtgcg cgcgcatccg tcccgttcgg gatcgcgtac
     4801 accttgcccc gaaacaccgt ttcgttccag cacgccgtgt aatactcgct cttgcgtatc
     4861 gcgtccggtg ccgtggtgtc ctgggcggca agccgcccgt ccagcggcag aaaggcatcg
     4921 cgcgccgccc agctcgacac ggtgaaacgg tcctggttca tcacatccgg cgcggcgcca
     4981 cccacgatgg cggtcagcat cttctgcggg ttctgtcccc cgccggaggg gctcgtggat
     5041 acccgcaccc cggggttcgc ccgctcgaat gcctctaccg ccgcgaaaaa cccgacctgc
     5101 ccctcccctt ctccaatacc ccacaccacg agcgtcgtat cgccgctgga cgcccacgac
     5161 gtggaaaaca acaggcaaat cagaagagcg cggaagcagt acaccatggc cctctcttga
     5221 ccggaaggaa attgcgtgcg ttcatcattc gactgaaata tggcaccacg accgcgcgcg
     5281 cggaaagggc attcccgtga aagaaccgtt cgattccctg cttcaccccg gccgcactct
     5341 cgtcatggga gtcgtgaatg tcacccccga ttccttctcc gacggcggca ggtttctctc
     5401 ccccgaagcc gccatcgcgc acgcttgccg cctcatggac gaaggcgccg acctcctcga
     5461 catcggcgga gaaagcaccg cacctgatag cccccccgtc gacgcgcagg aggagtgccg
     5521 ccgcattctg cctgtccttg aggaatgcgc gaaacgcggc ttcccggtgt cggtggatac
     5581 ctacaagtcc cccgtcgccc gctgcgccct cgcccacgga gccggaatga tcaacgacgt
     5641 aacagccctc cgcggcgatc caacacttgc tgacgtgctc gccgattccg actgcgccgt
     5701 cgtcctcatg ttcgccaagg acccgacccc ccgtaccacg ccaaccgtgc gcgtatacgc
     5761 cgacgtcgtg cgggaaatct cggagttcct ggccgaacgc gtggcgtacg ccggaacccg
     5821 cgggatcgct gccagccgca ttgtactcga ccccggcatg ggcgccttcg tcagcgctga
     5881 ccccggaccg agcctggaaa tcctccgccg cgcgggcgag tttcacacac tcggacaccc
     5941 gctcctgatc ggcgcatcgc ggaaaagctt catcggaaag gttctcggtg gccccgggca
     6001 gcctctcccg gtgggcgacc gcctcgaagg ctccctcgcc tgtgccgcca tcgccgcctg
     6061 gcacagggtc cagatcatcc gcgcgcacga cgtccgcgaa accgtccgca ccgtccgcat
     6121 ggtggatgct attcgcgtag cggacgctgt ctgaatggcg cctcacccca ggtctgcttg
     6181 acacgccgcg tggaggggcc cgcttcgtcc gaccctttct tcgcagcgca accaccggca
     6241 cgttgccttc attctgcgcg tgaatttgtt actattcaaa acagcagcgt atcttgaatc
     6301 aaagacgtac accaaggcgg acccggaagg ttcgaaatgc ctgaattgtc acgtttcttc
     6361 gggattaccg tgaggatgta ctacaacgac cacgaacctc cgcacttcca tgtcttttac
     6421 ggtgacttcg aagctctgat cgaagtcgga agcctccacg tatttcgggg ctctttaccg
     6481 cggcgggcat ttgcaatggt tgtggaatgg gcaatcatcc atcgagagga gcttgtcggg
     6541 gcgtggcagt ctgcccggcg cggcctcgcg gtgccaccga ttgttccctt ggattaggag
     6601 cttggtcaat gagcatgcag aggatcaaag cggttgaacc tctggacggg ttcaaactga
     6661 aactgacctt gactgacggg tcggtcatcg aacgagatgt ttcccgtctg ctggtcggaa
     6721 aggtgtttga gcctcttctc gccagccgca ctgagttttg tcgtgttcgt gcggaagctg
     6781 gcaccgtggt ctgggaaaac ggtgcggacc tgtgtccgga tgtactgatc tggggagggc
     6841 cgccgccaca ggacgaacgc cccggcattg ccaacccgcc agtatcagag ctgaagcccg
     6901 cgtagaggtg gctgatctgt cctatgcgtc ccataggacc tgtgccgcac gacagggaaa
     6961 ggcaccgccc accccggtca tcgagtggcg aacgccgcgg aggtttctct cctgcggcgt
     7021 tcgttgtatc gagatgacca ccccgttcac ccccaccgcg catcattcgg gcagaccata
     7081 ccccagtccc tgcgcagggc gtccatcgtc cgcatgatgg caagcgattc gtccagcggc
     7141 atcaccgcgc tctcggtctt cccctcgcgg aggcatcgca ttacttcctg cgcctcgaat
     7201 cggtacggcg tcccctcgca ggccacgtca atccgcaccg gatccttccc cgcttccgtc
     7261 agaacaagcg tcttcggatt gaggaacgac ggttccatcg tcaacgtgcc tgcactgccg
     7321 gcgatgaagg caccgcccgg cacccgtgtc cgcacgccga acacggcgct ggcgatgcgc
     7381 ccgtcgtcat agccgtagca taccgacgcc tgttcgtcca ccccggtctc cccgatatgc
     7441 gccacaccgg tcacccgccg tgcctgcccg aacagcatgg agatgtacga catcgggtag
     7501 atgccgacat cgaacatgct gcctcccccg agcgccggat tgagaaagcg gctcagcttc
     7561 gtttccgggg tcatgtcggt ccagaacccg aggcttgcct ggatcagtcg cggttcgccg
     7621 attgcaccgc cggaaacaag ctcccgcacc ctgatcgtcg ccggcaggaa gcgtacccac
     7681 atcgcctcca tgaggaacag ccgcttcgac cgcgccagcg cgatcagctc ctccgcttcc
     7741 cgcccggtga tagtgaatgc cttctcgcac aggactgctt tgcccgcgtt caggcacagg
     7801 accgcgttat cgtaatggaa tgagtgcgga gtgccgatgt agacgacatc cacgtccgca
     7861 tccgccacca gtgcttcgta cgacgcgtac cgccgcggga taccgaactc gtcgccgaac
     7921 gcatccgcgg tctgttgggc gcgggaaccg acggcgagga tttccgcctc atccggcagc
     7981 tctttcagcc cctgagcaaa cttgtgcgcg atcttccccg tgctcatgat gccccagcga
     8041 attttccccg tcatatcatt tgtctccacc ggcggttatc tgcatcgcgc aagaatgcgc
     8101 tccatgttct tgccaagcat cagctccagt tcttcctcgg aacagcgcga gtagcacatc
     8161 cacccgaact gcgggatcgg gtcgcgcatc ggggcgtccg tcccgaagag caccttttcc
     8221 gctccgacgt gtttcaccat gtattcgatg atgcgatagg tcaccggcgt cagcgtgatt
     8281 tcgaggtaga cgttctcccg ttccttcacc agcggcaacc gttcccgcgc ggtggggtaa
     8341 tcgccccccg aatgcgccag caggaagctg atctccggat aacgcttgct gatatcggcc
     8401 acctcgttga tgaaattgcc gcccgacgaa tggagcagac agaacaaccg gtgctcgttg
     8461 ccgtaggcat accactggtc gtaccgcggc gagttgtacg gaatccggac ccgcggatta
     8521 tacggcttca tgcctttgaa accccactcc tcgtgggcca gacgcatcca ccggtcgaac
     8581 tcctccgcag tgagataatt cgggtcgagc gtcgcatacc cgatgatgtc gtccgggaac
     8641 tgatccatgg cgtccttgag gatgacgttc ccctcttcgc agtcggcgaa tatgcccatc
     8701 caggagctta tggcgacttt gtcaatgccg aggcgccggt tgcggcggat catgtgcttc
     8761 gcgtcgcttc gcggcatcgg catgtacccc acgccgcgag catcgttgtg cgacatgtgc
     8821 gcgtgcgagt cgatcaccgt gatgtgctcc agcggcagcc cttccttcac gcggctcagg
     8881 atgggcccgt ccgtccgcgc aggatactcc tccggcagcc gttccagccg caacagtcgg
     8941 gcgaggttgc cccctgcaat gagcttccgg tccgtctcgc ccagctcggc gtagtccacg
     9001 aacgacttgg ccgcgcccat gcttttgagc agcgcatccg tgccgaaaag cagccgttcg
     9061 gcgccgtatc gctcggtgaa ctcctccagc gcgtagttcg cctgaaacgt cgagaactcg
     9121 atgtgcacgt tggggcattt gtccatgagc ggcaggatgt tccgcgtact ggcccatgaa
     9181 cagcccgtca tgaggatcgg cagcccgggc cgccgcgatg cgatttgaaa caggtcgccg
     9241 tacgacgtct gatcaatgcc gatggagagg aggatctgct ctcccgccag tgcatcgagc
     9301 agttcaccgc agacgtactc ctccagcagg tagttctgcg agcgcggcgc catacggacg
     9361 gccctcaccc cggcggccag catctctttc acaagccgtt tcggcggagg catttccccg
     9421 caatcatgcg gcagcaccac ccagcagggg aacagccgcg gattgccgga aatcgcctcc
     9481 atgagcatcc ggttgccgta cgccgggtca tactcccgcg ccacattgtg gtacacaagc
     9541 gctccgtgaa tgccgcacca cgccatttcg tcgaggagtg tctccagttt ccacgacact
     9601 tcctcgtcct tgggacccat tttgccgaaa gacgccagcg cgtccagata catgattccc
     9661 tgcatggcta tgccttccct tcaaaaagcc gcggacaggc ggatggattg cacttcatga
     9721 gaatacggcg cagcgggccg cggagacaac gcaaagggcc gccgccattc cggcacatga
     9781 gagatgaggc gacgcgtcgt cttcgtgtca agggttttcg cgaaaatggt tcaatcgggg
     9841 tacggttttt tctggggtgg gtagaaaatg tggcgcggcg gactacgcgg cgttccgatc
     9901 ttgccatacc gcggcgccgg ccgtactttt tccatagacc acttcggtgc cccggtagct
     9961 cagttggata gagcacaggt ttcctaaacc tggtgtcgct ggttcaattc cagtccgggg
    10021 taccagctta tctctcgccg ttatcttcgc tctgccacca gttgttttga aacagccacg
    10081 aacgtccggc tgatcgcggc gccgcaggtt ttctcgtaga acaccaccgg tcccacccag
    10141 gggatgatgg cttccgcgtg tccctgtgcc gttagatcgc gcagacagcg tttcagcaac
    10201 gtgccgccga taccgcgcgt gcgcttttcc ggatgggtgc ccatgggacc gaaccatgcc
    10261 atgccggggt tgttcgcgtc gtacgcggca aacccgacca cacccgcggc ctcaacggca
    10321 aggtggaggc tgattggttc acgcagaaag cagtgtgcga cctcgggcgt ccatgagggg
    10381 aactgggtgc ggacgaattc gagcgcgtgg gagagatccg ttcgggcggc gcggatgacg
    10441 cgcactccct gtgcggccag ccggtcctct tcctcatccg tccgccagtc tgccgtggtt
    10501 aacggacagg ccatgttcac gcgctgttca ccttcgacgt acccccgttg cgcgaggaag
    10561 acgcgggcct ccgcgtaccg tggatccaca cccggcatgg tgtaattggg cactgaatcc
    10621 gcgatactga ccgaccgaac tccccggcgc gccatcaccc gctcgatctg gtcaaagagt
    10681 ttcgtcgcga tgcctgttcg gcggtgggtc tccgccacgg cgaaccattt gaaccatccc
    10741 cgcaccggtg catgggatgg acgcgccacg ccatgcgcga accccaccag attacccccg
    10801 tcaatggcgc agagggtgag cgcgctgtcg taatccgggt cggcgaaggc cttttctttc
    10861 aaccagcggg gagtgacgcg gtcgtgtggc agcgagtcgt ccaggagttg ggcaaccgga
    10921 acgatatccc gcggttcaag gggacggatg tacaggcctg gatgtcgcat cggaacggtc
    10981 ggctcaaaga ccctgaacga caggtggcgc ggctctggct agcacttgtt ctggggccat
    11041 tcaggcggac gccacttatt acaagccttg cagccagagg ccccgcaggc cggcttctat
    11101 ctgctcgcga tgggtggcaa ggtagttctc gacggtggcc tgttgatccg gagtgagaat
    11161 acggcgcgcc tgtgaccagc cgtcgtcaaa cagggcttcc acgcgcatgc ggcggaccac
    11221 cgggtcggag attgtcagca gcgtcgccag atcgccggtg tacacctttg ccagcgactt
    11281 catctgcgcc ttctgagatg ccgtgaggtc gagttgcccc atcacgtcgc gaaccatcgg
    11341 gtcgctgagg tagtcggtcc agttcttgcg gagagtggcg acggttgaac gcgaatcctg
    11401 cagctttttc cactgttccc gggtcaatac ggcgcggatg ctgtcgcgga gcgcatcggc
    11461 ggcagcgggt gccgggtccc tgcggcgggc aggcgagtat ccgaccggag cggcggaatc
    11521 gccgggggtg ctctcgcgca gcctgcggta taaccgggcg atgtgttcct gctggacgga
    11581 atcgagccca aggtccgcga gcaccttctg tcccaccacc gaagccgcga ggccgggaag
    11641 ggacagggta tccgcgtggg cgcgttcgaa tgtgacttcc cggaaggtgc gggcaacgga
    11701 aaacaggcgt cgcaccgatt gggttccggc aacggccaga gcgcctgcta ttgctatcgc
    11761 aagaaaaatc aacgctttcc gcattacgcc tcactgccgc cggtcggcag ccctgtccgt
    11821 tcgatcacgc ggttcaatta cccgcacttg gtgctcgccg gcgccttctg gatacttaac
    11881 gatacggact caggagaaca gttcccaaca aagcagggct tatgccggtt tcgaaaagct
    11941 catcgctgac gacgtcggaa cgcatgcgcg aagaggccgc ttcgatcacg cgtgtcacga
    12001 atatcggcat cgtgatgaac gtgatcatga ctctggcaaa gatacttgcc gggtgggcgt
    12061 ttggttccat cgcgctggtt gccgacggag tgcactcgct ttccgacctt gccacggatg
    12121 ccctcgtgat cgtcggaacg cgcgtggggt tgcggccggc ggataagcgc cacccgtacg
    12181 gccacggcaa gatcgagacg atcagtgccc tgctcatcgc cttcgcgctg gtgtttgtcg
    12241 gcgcgcggat ttcgtttgtg gcagggcggg agatgtacct ccgccatcac tcctaccccg
    12301 gcatctgggt actcgtgatt gcggccattt cggtggtggg caaggaatgg cttttccgga
    12361 tcacgcgccg ggtggcgctg gcatgcaaca gcgccgcagt gttcgccaac gcctggcatc
    12421 accgttcgga cgccctcggc gcgctggccg tggtgttcgg ttccgtggcc gggatgatgg
    12481 ggtggggcca cggtgaccag acggccggcg tggtggtggg aatcatggtg gtcggcgtgg
    12541 ggctgaaaat cggcgtggac gcgctggtgg agcttttcga cgtaagcgcg gacgtcgaaa
    12601 cggttgccgc gctggaacag gcaatttcgg gcgaggaggg ggtccgcgcg tttcaccgcc
    12661 tgcgcagccg tcttgtcggc cgggagatat tcgtggacgt acacattctg gttgacccgg
    12721 cattgagcgt ggttcagggg cacagggttg ccgatcgggt ggaagaagcc attcgatctt
    12781 ccatgaagcg gccggcgaat atcgtcgtcc acatagagcc cgaccttccc gagcttcgca
    12841 ccgtgaaccc gaacaaataa ccatccgccg ccgcactgcg ccttcgcctg agcttttcgg
    12901 cgtggcgatt ttcccgcgcc accacccgcg gaattggttc ggtggcgggc aggcgatata
    12961 ctttgttgca tgaaaaactg ctcgcggagt acggcaaaaa atgccggaac cgccggcccc
    13021 attggaaaag gagcctcacc gtggccaaaa ttcccattgc gattcagatg tacacgctgc
    13081 gcgaggactg cagcaaggac ttcgtcggca cgttgaaagc cgtcgccaaa atcgggtacg
    13141 ccggtgtgga actggcaggg atgtacgggg ttgcgccgaa ggaactcgga aagatactgg
    13201 atgacctcgg cctgaagcgc gcgggcggac actggggcgt cgaaccgctg gagaaggaat
    13261 ttgacaagac ggttgaagac gtgaaggcgc tcgggtcgtc gtttgtgacc atccccggtc
    13321 ttccgggcac ctacaccgag gacgccgaag cgtggaagcg caccgccgtt ctgttgtcag
    13381 ggatcggaca gaaactgctg aaggaaggca ttcagctttc ctaccacaac cacaaccacg
    13441 agttcaaact ctttggcgga aagcacggcc tggacattct gtatgaaaac agcgatccga
    13501 aggcactcca cgccgagatc gacacctact gggtgcagta tgcgggcgtt gacccggcca
    13561 cgtacatgaa gaaggtcgcc aaccgccttt cgctggtgca tatcaaggat atggcggacg
    13621 acgatatgaa gtcgttcgcg gaaatcggca acgggatcct cgactgggac gcgattttcg
    13681 ccgtcgccaa agaagccgga tcgaagtggt tgatcgtgga gcaggattcg tgcaagcgcc
    13741 cgccgctgga gagcgcgaaa atcagctttg agaacctgaa aacgatgggc gcggtgtagt
    13801 caaccggatg gtgttcactt gccacacacc ggtttcgtgc ctatcttttt agcggcatta
    13861 tttgggaaac aggggctgta gctcagctgg gagagcgcct gtctggcaga catgaggtca
    13921 cgggttcgat ccccgtcagc tccaccatgt gacaatacgg gacttgcgag cgttcgtgag
    13981 tcccgtattc cgttgacatc gtcttcacgc cacggttacg gagttctgcg cc
//
LOCUS       DAOSOO010000067        14001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4380_length_14001_cov_5.552775, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000067 DAOSOO010000000
VERSION     DAOSOO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_14001_cov_5.552775"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..695
                     /gene="pgsA"
                     /locus_tag="PLT86_06540"
     CDS             108..695
                     /gene="pgsA"
                     /locus_tag="PLT86_06540"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT36303.1"
                     /translation="MAKIREFGESANLITLARIVLTPLFVWWFTSADWRLKVLGFVVF
                     VAAAITDWWDGHHARKHKTVSNLGKFLDPLADKLLTIAAMLTLAYEHLVQLWMILVIF
                     ARDFWLTWLRVRAANAGKAFVTSRLAKFKTAVQMTAIITIIGLWCIYSIASTYQMWPG
                     IVPEYWLVALFNVMIAATMLLALVSGFQYLRRSTG"
     gene            705..1211
                     /locus_tag="PLT86_06545"
     CDS             705..1211
                     /locus_tag="PLT86_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOT36304.1"
                     /translation="MIIRREYDTRGVNAFQSISTKLIATVAGAGYSPVAPGTAGTLVG
                     CLVLFILEPDFSSVLFLVAIAVVYAIGVLVSSWAERVWEKSDPGQVCIDELAGLLVSL
                     AYVPAKDPALLLVAAFLLFRMFDIIKPPPARAAENARSGWGIMNDDMIAGLYTNAALQ
                     IISRLGWL"
     gene            1208..2533
                     /locus_tag="PLT86_06550"
     CDS             1208..2533
                     /locus_tag="PLT86_06550"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT36305.1"
                     /translation="MKRNTSLQTGEPPRAEIITVGNEVLSGRTVNTNAAFVASELERV
                     GVSVAWVTTVGDVSAHIRDAFLRAWERANVVIVTGGLGPTHDDVTRSVFCEVFARGLR
                     RDEAVLQNIRSLFAARGRPVTPRNEEQALVPEGTEVLQNRWGTAPGIHWTENGKHWFL
                     LPGVPREMQNLLTHEVLPRLRGLPGARTVVRRELHVVGVPESYLMDRIADITGIEQVA
                     SLPDSKGEVCLRVTVASESYDEAHARCARIVSDLEARLGDAIYGYDGDTLEAVVGRLL
                     AERNMTIAVAESCTGGLVTSRLTDVPGSSRYLVSAVVAYANAAKTDFLGVPLSTLAAH
                     GAVSAETAAAMASGIRNRSQADLGVAVTGIAGPGGGSPEKPVGLVYLAVADREGIEVV
                     RELFGDDRLLTKARTAEAALALVRKRLLRKMGNAVTHVNPKNREGNRAT"
     gene            2530..3234
                     /locus_tag="PLT86_06555"
     CDS             2530..3234
                     /locus_tag="PLT86_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT36306.1"
                     /translation="MNVLIVTAHPDDMEILCAGTAVRYALRGDSVTICHACTGSLGHM
                     VIPPEELVPIRWEEAEKAAKIAGLRHETLGLPDLQVTERDEVVSLLASLIRDVRPDVI
                     ITHDPNDYMPDHSDLAHLVLKSSFVATLPHHEHVNGECFAVVSPVFFMDTVMGVGFVP
                     EEYVDITDVFDTKKAMLACHASQVDWLRDHDGIDVIENMRVCAQYRGLQCGKKYAEAF
                     RVNRTWPRVTTERLLP"
     gene            3262..3828
                     /locus_tag="PLT86_06560"
     CDS             3262..3828
                     /locus_tag="PLT86_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT36307.1"
                     /translation="MGELLPTRDEAWALLCEWTASPGLRAHALAVEAAMRSYARNSGE
                     SEEYWGNVGLLHDFDYERYPDAADHPLKGAEELRRRGYDESFVRAVLSHADYLGLARN
                     TNAEKTLFAVDELCGLIMATAMVMPGRSLAEVTAEAVLKKMKSKGFARKVNREDIVRG
                     TEGLGIPLETHVSNVVRALQGIAATLGL"
     gene            3852..4967
                     /locus_tag="PLT86_06565"
     CDS             3852..4967
                     /locus_tag="PLT86_06565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT36308.1"
                     /translation="MATPVILPQMSDTMTEGTLVRWLKAEGDEVEAGDPIAEVETDKA
                     VMELEAPARGTLLRVFVSPDTVVSCGTRVALIGAPGEEGENEPPAAPPSTESRVHASP
                     AARKLASRSNIDIRRVLGSGPGGRVVLRDVAAAAAKVLKEPTPSSWEFPRQRSVMVSR
                     LVSSHQEIPTFTVTRTIQMDRALAFRKLQTESGAFPEGLGITELCVAAAARALGAEPR
                     LNARYSDGRVILQETVNIGIAVGLEDLVVVPVIQRCNALTLKRIATEYRRIVAAAKGG
                     SLLPDDARGGTFTVSNLGMLGVEQFSAIINPPEAAILAVGAIRREIYFELDRIEARNV
                     MSVTLTVDHRVADGLHAARWLQEFARALENPGQMLAI"
     gene            5060..5392
                     /locus_tag="PLT86_06570"
     CDS             5060..5392
                     /locus_tag="PLT86_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36309.1"
                     /translation="MEEHEIVAALAENRLHEEVDAIEQTSALNGIHQVLFSRALGLGI
                     PRAELVRRIDDATFQVARSSMLDINELKLMNRKTLEGYDPKLARDVYRAQIHVMIDVC
                     AEAAASAG"
     gene            5475..6194
                     /locus_tag="PLT86_06575"
     CDS             5475..6194
                     /locus_tag="PLT86_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36310.1"
                     /translation="MEKREILTALEEGRLRGIIKSFNSTRELEPVHQALFHKALELGV
                     PRVELVKAIDDSTFDVAKKAMLDHRDLKQMSRKMLEDYDPRLAVEVYRTQVNVMITAC
                     ETAMKQRAILKPEEHLFNTDAYGQTLSTAAVDGHPEVAVFTGLAKLDGETVTFGRIGA
                     DKAYNNLKANPHAVFTAVGPSNDPTKYAFITIDAHLDKDITEGPELEGLRKRLGSPVV
                     NCMVFSVSKINVVHLPKPKPA"
     gene            6200..6820
                     /gene="rdgB"
                     /locus_tag="PLT86_06580"
     CDS             6200..6820
                     /gene="rdgB"
                     /locus_tag="PLT86_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT36311.1"
                     /translation="MRCQIIAATRNRGKLREIQSLYADLPLTWRPMDDIGSPPIIEET
                     GTTFLENALLKARGIAQWSGCAALGEDSGLEVDALAGNPGVRSARFAGEHASDEENIA
                     LLLRRLDGIPSAERTARFVCVVALVWTDGTEVIAKGTCEGHIAEERAGASGFGYDPLF
                     IPEGQVATFAELGEEFKNTVSHRARALRALRPWLEGRIRYAGRAPQ"
     gene            6820..7608
                     /locus_tag="PLT86_06585"
     CDS             6820..7608
                     /locus_tag="PLT86_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT36312.1"
                     /translation="MELYVLASSSAGNAIAIRNGQGIMLVDAGLSARRIEERLHAVGW
                     HAQDVKCVLLTHEHSDHTRGVGAFARRYRVPVYGTPGTLQAITDQWRGSESLYAIAAG
                     SRTAIHGFEVEAFSVPHDVSDPVQFVIRDGLVSVGIATDLGTVTSLVRQKMRAVDLAV
                     VEANHDAERLRWGRYPWNVKQRVGSSQGHLDNEQAGELVRDLAREGVPHIVLAHLSPQ
                     HNDAEHVEKAMVNALATVAQRPVLTIVPPGEGSPVIQVRKAVRS"
     gene            7605..8627
                     /gene="rfbB"
                     /locus_tag="PLT86_06590"
     CDS             7605..8627
                     /gene="rfbB"
                     /locus_tag="PLT86_06590"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT36313.1"
                     /translation="MSWTILVTGGAGFIGSNFVRYLLEKYPGYRVINLDKLTYSGNLN
                     NLKDIARDPRYEFVHGDIAEESVVLPLVRRSDAVINFAAESHVDRSIADPGVFLRTNI
                     EGPHVLLEASRKSGVKRFVQISTDEVYGEVMTGSSRETDRLAPRSPYSASKAGGELLV
                     QSYHVTFGVPTLVTRGSNTIGPYQYPEKVVPLFITNALDDRPLPIYGPGTAVRDYIHV
                     VDHCRGIDQVLHHGVEGEVYNIGADNSVNTVDLASAIVDYLGKPRALLQHVQDRPGHD
                     QRYSVDSGKLRKLGWNLSYTFEDALEETIRWYRDNRDWWEPIKRGEYARWYDTHYKIR
                     EIPHES"
     gene            8617..10236
                     /locus_tag="PLT86_06595"
     CDS             8617..10236
                     /locus_tag="PLT86_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOT36314.1"
                     /translation="MNRSNILMLLSAVALLFSTQVAALTPWAGDEEERQDRVPVPEER
                     DRTGQQGVSRAVSATPLDARVDREKYRLDPGDRVLVSLWGATNKSFELVVTPEGDLLV
                     PAVGPVAVARLSLAEAERTLARQAANAYRNTRITLSLVALREFRVHVAGTVKRPGTYL
                     GTPADRVSSVIRLAGGFADSASMRRIQLLRTGLPPRLIDLQMFSVTGDTTDNPSLEMG
                     DVITVPVRGDTVSVWGAVRKPGGYEYRPGDTVLRLIRLAGGLRDDAQTAEVEWSSFPV
                     DSAPTVVNKVSLDRLIATGSDTVVRPGDRLMVYPVAEWRRRQSVTIEGEVRFPGEYSI
                     IEGKTRISDVIARAGGPTRSASLVGSRILRRRVQAEPDQEFLRLSRMPVADMKREEYA
                     YFKARSRELRGYLSIDVGTALESPGGVDDAPLVDGDLIVIKRARATIEVAGQVRRPGL
                     IEFDEGRSTQFYIDQAGGVLSNAHRRGIRVIKQGTGLWVKPSADLRLEPGDTIFVPEK
                     ESVEWWELFKEGLLVVSQIATTVFIIQSVVR"
     gene            10236..11414
                     /locus_tag="PLT86_06600"
     CDS             10236..11414
                     /locus_tag="PLT86_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36315.1"
                     /translation="MDAHIENTPAAEGGKSAFASFLALLWRGRLVIVGTFVVVVTVVT
                     IAVFVMTPSFTAKASFMPPPDDAGSVSSFLRDPLSAVLKRGSGGAVLDRLASFLNSQT
                     TKRILVERFNLWPHYKARNLSEAMIALEKQTRVVVTTEGTIELAVTDRDPRLAAALAN
                     CYLDVVDSLFRDAQTSHARQMREFLERRLNENRRALAEAESTAKAFSLRYGVVALPEQ
                     VASLVGLMASIEGKIMALDVQIGAARQVLGPDHFSLRELTSQREQLAAQRKALIETKG
                     RGTSDPLFAFREVPERALQWARIEREIKIQTLIQELVLQQYEVAKLEEQRDVSSLTII
                     DRATPPEIRSWPRRGRIIAISAAIAVVWGILLAYALEEYPRLRRRIREMNDLHSRSDA
                     "
     gene            11431..12861
                     /locus_tag="PLT86_06605"
     CDS             11431..12861
                     /locus_tag="PLT86_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT36316.1"
                     /translation="MNTRRLTGEAAVYTFGIVLDTLAPFLVLPILGRYLTPGQFGLAD
                     VITATAGLTYFAVGFNLDQALTRYYYDEEDAHRKRLVTTHLAVVSVSGTVFLVLGLVG
                     LHAFRGSLQDLSAWHWALLSVPLMVMSDQAATLFRLQHQPFKQLGLLSVRAFVWVFFT
                     SFFLVWLRAGISSIFIGRVAGAIFGLGASAYALRRLYALRGNLRFAVRALRFSLPTLP
                     AAFASWGLVNAARYFLVIGASAEETGYYGVGVRLTYIVSTIGIGVVMAWGPFFMGIKE
                     EPNAREIQARGLLYFIAANVVCIAPLLMFSREAVLLLMGSQYLPAVRLVGPMLAATLF
                     SNLSLFLFVQISVSERTHWQSVSYAVGLVVMVIANVLLVPAMKGMGAAIAMLCGHLAT
                     AGTMWFAGQRVYTVPVSVARVGILILVLAGCLVLAPTTTTRFGTTLQGIALRALLTVL
                     MWSACLAAVGPGDVLRFAATMVGKRT"
     gene            12858..>14001
                     /locus_tag="PLT86_06610"
     CDS             12858..>14001
                     /locus_tag="PLT86_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36317.1"
                     /translation="MNVAGRLSSGADSFHQLAIAGATSAALVGAVFMLPPEVSAFLFY
                     VSAGAIILCIWIVRSAPREDVTGLLVGALCALILRAALAWWVFDHPERYSDTRLYWTL
                     GNRIASLWEPGRILPVEMVKATGTSSWGFFFWSGLHISILRTPLLLVAANGIAGTAVG
                     VMAYHIGRHVWSREVGRRAAWLVWASTSQLIVDAHLLRDSGAILFGAGVVYGSLLLAD
                     RLSLRGLLWYGISFFLLMQMRSYIAMVVAPASLLGVLIGARGRRLSLLLIYLVAGTGA
                     AFFLASTDFVRILTALGRKNVIADMLYLTQVGLSGIRPSASYFSGVHLSTPREMIAFL
                     PFGTMRELLSPLPWMPIEMDRWFIPEGLLRWTLIPLFVLGVVEALRK"
BASE COUNT         2798 a         3919 c         4370 g         2914 t
ORIGIN      
        1 cggcggctcc cttaaccatg cagacaaggt taccatcggg ttatcgctcg atggacgggt
       61 tcaactcacg taggacgagc tagcaatagc tcgctttgca gggttttatg gcgaaaatca
      121 gagaattcgg cgaatctgcc aacctgatca ctctggcccg aatcgttctt actccgctgt
      181 tcgtctggtg gttcacttcg gccgactggc ggcttaaggt tctgggattc gtcgtgtttg
      241 ttgccgccgc gatcacggat tggtgggacg ggcaccatgc cagaaaacac aagaccgtct
      301 cgaatcttgg caagttcctc gatcccctcg cggacaaact gctcaccatc gccgcgatgc
      361 tgacgctggc ttacgagcac ctcgtacagc tctggatgat tctggtgatc ttcgcaaggg
      421 atttctggtt gacctggctg cgtgtgcgtg cagcgaacgc gggaaaagca ttcgtaacgt
      481 cccgacttgc gaaattcaaa actgcggtgc agatgaccgc cataatcacg attatcgggc
      541 tttggtgcat ctacagcatt gccagcacgt atcagatgtg gcccgggatc gttcccgaat
      601 actggcttgt cgcgctgttc aacgtgatga tcgccgcgac gatgttgctg gcacttgtat
      661 ccggtttcca gtatctccgt cggagcacag gttaggagcc gaggatgata atccggcggg
      721 agtacgacac gcgaggcgtg aacgcctttc agtcaatctc cacgaagctg atcgccacgg
      781 ttgccggggc cggttactcc ccggttgcac cgggcaccgc agggaccctt gtcggatgcc
      841 tggtgctgtt cattctcgag cctgattttt cctccgtcct ttttctcgtt gccattgccg
      901 ttgtgtacgc gatcggcgtt cttgtcagtt catgggccga acgcgtgtgg gaaaagagcg
      961 atcccggcca ggtatgtatt gacgaattgg ccgggttgct ggtatccctc gcgtacgtcc
     1021 ccgccaaaga ccccgctctt ctcctcgtcg cggcgtttct tctgtttcgc atgtttgata
     1081 tcataaagcc gccgcccgca cgcgcggccg aaaacgcacg gtccgggtgg gggatcatga
     1141 acgacgacat gatcgcaggg ctctacacga acgcagcgct gcaaatcatc agtcgactgg
     1201 gctggttatg aaacggaaca catcgctgca gaccggcgag ccgcccagag ccgagattat
     1261 cacggtaggc aacgaggttc tctcggggcg cacagtgaac accaatgccg cttttgtcgc
     1321 atcagaattg gagcgggtgg gtgtctcggt ggcatgggtt accacggtgg gagatgtttc
     1381 cgcgcacata cgcgacgcct tcctgagggc atgggagcgc gcgaatgttg tgattgtaac
     1441 cgggggactc ggtccgacgc acgatgacgt gactcgttcg gtgttctgtg aggtcttcgc
     1501 gcgcggcttg cgacgggatg aagcagtgct gcagaacatc cgcagcctgt ttgctgcgcg
     1561 cggcaggccg gttactccca ggaatgaaga gcaggccctt gttccggaag gaactgaggt
     1621 gcttcagaat cggtggggca ctgcgcccgg aatccattgg acggagaatg gaaaacactg
     1681 gttcctgttg ccgggtgttc cgcgcgaaat gcagaacctt ctgacgcacg aggtgctccc
     1741 cagactcaga ggcttaccgg gcgcaaggac cgtggtgcgg cgggagcttc acgtggtcgg
     1801 cgtgcccgaa tcctatttga tggaccggat tgcagatatt actggaatcg aacaggttgc
     1861 atcgcttccc gactcgaagg gcgaagtatg tctccgcgtc accgtagcct ccgagagtta
     1921 cgacgaagcc cacgctcgat gtgcacgtat cgtctctgat cttgaggcca ggctcgggga
     1981 cgcgatttac gggtacgacg gcgacaccct ggaagccgtg gtaggcaggt tgctcgccga
     2041 acgaaacatg accatcgcag tggcagaatc gtgcacaggt ggcctggtaa catctcgctt
     2101 gacggacgta cccggaagtt ccagatacct tgtttcggca gtcgtggcat atgcgaatgc
     2161 cgcgaaaacg gacttcctgg gagtgccgct gtcaacactt gccgcacacg gcgcggtaag
     2221 tgcagaaacg gcggccgcca tggcctccgg aatacggaat cgatcgcagg cggatctggg
     2281 agttgcagta accgggatcg ccggaccggg cggagggtct ccggaaaaac ccgtcgggtt
     2341 ggtgtacctg gcagtggcgg accgcgaggg aatcgaggtg gtgcgcgagc tgttcggcga
     2401 cgaccgcctg ctcaccaaag cacgaactgc ggaagcggcg ctggcgctcg tgcggaagcg
     2461 gttgctccgc aagatgggaa atgccgtcac gcatgtgaat ccaaaaaacc gggaggggaa
     2521 cagggcgaca tgaatgtcct tatagtgacc gcacaccccg acgacatgga gatcctgtgc
     2581 gccggaaccg ccgtccgcta tgcgttgcgg ggagacagcg tgacgatctg ccacgcgtgt
     2641 acgggttctc tcggacacat ggtaattcca ccagaggagt tggtgcccat acgctgggaa
     2701 gaagctgaga aagctgcgaa aatcgcgggg ctgcggcacg aaacgctcgg attgcctgat
     2761 ctccaggtca cggaaagaga cgaggttgtc tccctgctcg catctctgat ccgcgacgtg
     2821 cgcccggatg tgattatcac acacgatcca aacgactaca tgccggacca cagcgatctg
     2881 gctcacctcg tgctgaaaag cagcttcgtg gcgacactcc cccatcacga acacgtcaat
     2941 ggcgagtgct tcgcggtggt atccccggtt tttttcatgg acaccgtgat gggagtcggt
     3001 tttgtccccg aggaatacgt ggacatcacc gacgtcttcg acacgaagaa ggccatgctg
     3061 gcctgccatg ccagtcaggt tgactggctc cgagaccacg acggaatcga tgtaattgaa
     3121 aacatgcggg tctgtgccca gtaccgcggg cttcaatgcg ggaaaaagta tgcggaagcg
     3181 ttccgcgtga atcgtacgtg gcctcgggtg acaacggaaa ggcttctgcc gtaacaccgt
     3241 tccgggaaag cgaggggata tatgggtgaa ttgcttccca cgagggacga ggcatgggct
     3301 ctcctctgcg aatggaccgc gtctccgggc ctgcgcgccc atgcgctggc cgtggaggcc
     3361 gccatgcgtt cctacgcccg gaattccggg gaatcggaag agtactgggg caacgtcggg
     3421 ctgcttcacg acttcgacta cgagcgctac ccggatgcgg cggatcatcc gctgaagggc
     3481 gcggaagaac tgcgccgtcg cgggtacgac gagtcgttcg tgcgcgccgt tctgagccat
     3541 gcggactacc tcgggctggc ccggaacacg aacgcggaaa agacactttt cgccgtggac
     3601 gaattgtgcg gtctgataat ggcgacggcg atggttatgc cgggtcgctc actcgcagaa
     3661 gttaccgcgg aagcagtgct caaaaagatg aagtccaaag ggtttgcgcg gaaggtgaac
     3721 agggaagaca tcgtccgcgg aaccgaggga ctgggtatcc ccttggaaac gcacgtatcc
     3781 aacgtcgtcc gggcactgca gggaatcgcg gcgacgctcg gtttgtaact tcgggcggga
     3841 aaggcatcac catggcaaca cctgtcattt tgccgcagat gagcgatacg atgacagagg
     3901 gcacgctcgt acggtggctc aaggctgaag gggatgaggt tgaggcgggt gatcctatcg
     3961 ccgaggtgga aactgacaag gcggtcatgg aacttgaagc gcctgcacgg ggaacgcttc
     4021 tgagggtttt cgtctcgccg gatacggtgg tctcctgcgg gacgcgcgtc gccttgattg
     4081 gagctccggg ggaggaagga gaaaacgagc cccctgccgc tccgccttca accgaatcgc
     4141 gagtgcacgc ttctcccgcc gcacggaaac ttgcctcccg cagcaacata gatatccgtc
     4201 gggtattggg ttccggacca ggaggacgcg tcgttctccg agacgtggcc gccgcagcgg
     4261 cgaaagtcct caaggaaccc accccgagct cgtgggagtt cccccggcag cgttccgtca
     4321 tggtttcacg tttggtgtct tcccatcagg aaatccccac gttcaccgtg acacgcacca
     4381 ttcagatgga ccgcgcactt gcatttcgga agctgcagac agaaagtggc gcgtttccgg
     4441 aaggactcgg aattaccgaa ctgtgcgttg cggctgcggc gcgagccttg ggagcggagc
     4501 cgcgattgaa tgcccgctat tccgatggcc gcgtgatcct gcaggaaacc gttaacatcg
     4561 ggattgcagt gggattagaa gatttagtgg tcgtccctgt cattcaacga tgcaatgcgc
     4621 tgactctgaa aaggattgca acggaatatc gacgaatcgt tgcagccgca aagggcggtt
     4681 ccctgttgcc ggatgatgcg cgaggcggta cgtttacggt atcgaacctg gggatgctgg
     4741 gtgtcgagca gttttcagcg atcatcaatc ccccggaagc ggcgattctt gccgtgggcg
     4801 ctatccgcag ggagatttac ttcgagctgg accggatcga agcgcggaac gtgatgtcgg
     4861 tcaccctgac ggtggaccac agggtcgcgg atgggctgca tgccgcacgt tggctccagg
     4921 aattcgcgcg ggctctggaa aatccggggc aaatgctcgc gatctgaacg gacacgggtt
     4981 ggcgacgcgt tttcgtatat tttggtgcgc gtggacaatt gggaagaatc gtagccttgc
     5041 ttagaggggc cctttggcga tggaagaaca cgaaatcgtt gctgctctgg cggaaaaccg
     5101 acttcatgaa gaagtcgacg caatcgaaca gacaagtgcg ctgaatggaa tccaccaggt
     5161 gctgttttcg cgagcgcttg ggcttggaat tcctcgcgct gaactcgtgc gcagaatcga
     5221 tgacgctaca ttccaggtcg cgcgttcctc aatgctggac attaacgagc tcaaactcat
     5281 gaatcgaaaa acactcgagg ggtatgaccc caaactggct cgcgacgtat accgcgcgca
     5341 gatccatgtg atgattgatg tgtgcgcgga agcggctgcg agcgctggtt gaacaggagc
     5401 ggcaccgaat ggcccctgtt cattgaaata caagtttctt cagacagtca tttcccgttg
     5461 cccagggagg taccatggaa aaaagagaaa ttttgacggc gctcgaggaa ggacgccttc
     5521 ggggcattat caagtcgttc aactcgacgc gagaactcga accggttcac caggcgctgt
     5581 ttcacaaagc actcgaactg ggcgttccgc gggtggagct tgtcaaagca atcgatgaca
     5641 gcacgttcga cgtcgcgaaa aaggcgatgc tcgatcatcg cgatctcaag cagatgagca
     5701 ggaaaatgct tgaggattac gacccgcgac tggcggtaga ggtgtaccgg acgcaggtca
     5761 acgttatgat taccgcctgc gagactgcaa tgaaacagcg ggcgatcctc aaacccgagg
     5821 agcacctttt caatacggac gcctatggcc agactctctc cacggcagca gtggacgggc
     5881 atccggaagt agctgtcttt acaggtcttg cgaaacttga cggtgagaca gtgacgttcg
     5941 ggcggatcgg ggcggacaaa gcgtacaaca atctcaaggc gaacccccac gccgtcttca
     6001 ccgctgtcgg cccgagcaat gatccgacga agtacgcgtt catcaccata gacgcccatc
     6061 tggataagga catcacggaa ggtcccgagt tggaggggtt gcggaaacgg ctcggttcgc
     6121 cggtggtgaa ctgcatggtg ttctcggtaa gcaaaatcaa cgtggtgcat ctcccgaaac
     6181 cgaaaccagc gtaacgggta tgcgctgtca aatcattgcg gctacgcgca acaggggcaa
     6241 actgcgggag attcaatccc tgtatgctga tctgcctctg acgtggcggc cgatggatga
     6301 catcggaagt ccgccgatca tcgaggaaac cggcaccaca tttctggaaa acgcccttct
     6361 caaagctcga ggcattgcac agtggagcgg ctgtgcggcg ctgggtgaag acagcggcct
     6421 ggaagtagat gcactggcag gcaatcccgg cgttcgttcc gcacgcttcg ccggggaaca
     6481 cgcatcggac gaggagaaca tagctctcct gttgcgccga ctcgacggga ttccctcagc
     6541 cgaacgcacg gcacgattcg tctgcgtcgt cgccctcgtc tggacggacg gcactgaagt
     6601 gattgcgaaa gggacctgcg aagggcatat cgcggaagag cgtgcgggag caagtggttt
     6661 cgggtacgac cctcttttca tccctgaagg acaggtggcg accttcgccg aactgggcga
     6721 ggagttcaag aacaccgtga gccatcgcgc cagagctttg cgggcacttc gtccctggtt
     6781 ggaggggcga atcagatacg caggtcgtgc accccagtga tggaactgta cgttcttgcg
     6841 agcagcagcg ccggaaatgc gatagcaatt cggaacgggc aaggcatcat gctcgtcgat
     6901 gcaggtctca gtgcccgacg catcgaagaa cggctgcacg ctgtcggttg gcacgcgcag
     6961 gacgtgaagt gtgttctcct cacccacgag cacagcgatc acacgcgggg agtcggggcc
     7021 ttcgcgcggc ggtaccgcgt tcctgtatac ggaaccccgg gaaccctgca ggctataact
     7081 gaccaatggc gcggaagtga gagcctgtac gccattgcgg cggggtcacg gaccgccatt
     7141 cacgggtttg aggtggaagc gttttccgtt ccgcacgacg tgtcggaccc ggttcaattt
     7201 gttattcgtg acggcctcgt gtctgtcggg atcgcaaccg acctgggcac agttacttcc
     7261 ctcgttcggc agaagatgcg agctgtggac cttgcagtcg tcgaagcaaa ccacgacgcc
     7321 gaacgattga ggtggggccg atacccgtgg aacgtcaaac aacgtgtggg cagctctcaa
     7381 ggacacctgg acaatgagca ggcaggcgag ctggtgcgcg atctggcacg ggaaggcgtt
     7441 cctcacatag tccttgcgca tctcagcccc cagcacaacg acgccgaaca cgtggaaaaa
     7501 gctatggtga acgcacttgc cactgtggca cagagaccgg tgctgactat cgttccaccc
     7561 ggcgaaggaa gtcctgtaat ccaggttcga aaggccgtgc gttcatgagt tggaccatac
     7621 tggtgacggg cggcgcggga ttcatcgggt ccaatttcgt gcgctacctc ctggaaaaat
     7681 acccgggtta ccgcgtcatc aacctcgaca agctcacata ttccggcaat ctgaacaacc
     7741 tcaaggacat cgcaagggat cctcgatacg agttcgttca cggggacatc gcggaggaat
     7801 ccgtggtgct gccgctggtg cggcggtccg acgcggtgat caactttgcg gcggaaagcc
     7861 acgtagaccg atccatcgcg gacccggggg tgtttctccg aacgaacatc gaagggccgc
     7921 acgtgttgct cgaggcctcc cgaaagagtg gtgtcaagcg ctttgtgcag atcagcaccg
     7981 acgaggtgta cggcgaggtg atgacggggt cttcccgaga aacagatcgg ctggcgccga
     8041 ggagtcctta ctccgcgagc aaagcgggag gcgaattgct cgtccagtcc taccacgtaa
     8101 cattcggtgt gccgacgctt gttacccggg gatcaaacac catcgggccg taccagtacc
     8161 cggagaaagt ggttccgctg ttcatcacga atgctctcga cgatcgtcct ctgcctatct
     8221 acgggccggg gacggcagtg cgtgattaca tccacgtggt cgaccattgc cgtggaatcg
     8281 atcaggttct gcatcacggg gtggaaggcg aggtgtacaa catcggtgct gacaactcgg
     8341 tgaacaccgt tgatcttgca tccgccatcg tggattacct cggcaagccg cgtgcgctcc
     8401 tccagcacgt acaggacagg cccggccacg accagcgtta ttctgttgac tcgggcaaac
     8461 tccgcaagct gggatggaac ctctcttaca cgtttgagga tgcgctggag gagacgatac
     8521 gctggtatcg cgataaccga gactggtggg aacccatcaa gaggggtgag tacgcgcgtt
     8581 ggtatgacac acactacaaa atccgcgaga ttccgcatga atcgtagtaa tattctgatg
     8641 ctgctatcag cagttgcgct ccttttttcg acgcaggtcg ccgccctgac gccgtgggcc
     8701 ggagacgagg aggaacggca ggatcgggta ccggttcctg aggaacggga ccggacgggt
     8761 cagcagggcg tttcccgcgc agtttcggca actcctttgg acgcgcgcgt agaccgggag
     8821 aaataccgtc tcgatccggg tgatcgtgtt ctcgtgagcc tctggggagc tacgaacaaa
     8881 tcgttcgaac tggttgtcac gcctgaaggc gaccttctgg ttccggcagt aggtcctgtt
     8941 gccgtagctc ggctttccct cgcggaggcc gagcgcaccc tcgcccggca ggctgcaaac
     9001 gcgtatcgca acacgcgcat cactttgagt cttgtcgcgc tgcgggaatt ccgtgtccac
     9061 gttgcgggca ccgtgaagag accgggaacc tatctcggca cacccgcgga cagggtcagt
     9121 tctgtaatac gcctcgccgg aggatttgcc gacagcgctt ccatgcggcg catccagctc
     9181 ctgcgcacgg gtttgccgcc aaggctgatt gatctccaga tgttttctgt cacgggcgat
     9241 accacggata acccttctct ggaaatgggt gacgtaatca ccgtgcccgt tcgcggggat
     9301 acggtgagtg tgtggggcgc ggtgcgcaaa ccgggaggat acgagtatcg cccgggggat
     9361 acggtcctgc gcctgatccg cctggcagga gggctgcgag atgacgccca gaccgccgaa
     9421 gtggaatggt ccagctttcc ggtggattcc gctcccacag tagtgaacaa ggtgagcctg
     9481 gaccgtctca ttgccacggg gtccgatacg gtggtgcgtc ccggcgacag gttgatggtg
     9541 tatcccgtcg cagaatggcg acgcaggcag tcggtaacga tcgaaggcga agttcgcttc
     9601 cccggcgagt actcgatcat cgaagggaag acgaggattt ctgacgttat tgcgcgcgcc
     9661 ggtgggccca ccaggagtgc gtctctcgtc ggctcccgca tcctgaggcg gcgtgtgcag
     9721 gcggagccgg accaggaatt cctcaggctg tcgaggatgc ctgtcgcgga catgaagcga
     9781 gaagaatacg cgtatttcaa ggctagatca cgcgagttgc gcgggtacct ttccatcgac
     9841 gtcggaactg cgctggaaag tcccgggggt gttgatgatg caccgcttgt cgatggcgat
     9901 ttgatcgtaa tcaagcgcgc tcgcgccacg atcgaagtcg cggggcaggt cagaaggccc
     9961 gggctgattg agttcgatga aggaagatcc acgcagttct acatcgacca ggcgggcgga
    10021 gtgctttcaa atgctcacag gaggggcatt cgagtaataa aacagggtac aggcctctgg
    10081 gtcaaaccat ctgcggacct gcgcctggaa cccggagata cgatcttcgt cccggaaaaa
    10141 gaatcggtcg agtggtggga gcttttcaaa gaggggcttc tcgtcgtgtc gcagatcgca
    10201 actaccgtgt tcatcatcca gagcgtggtc cgctgatgga tgcacacatc gaaaacaccc
    10261 cggcggcgga aggcggcaaa tcggccttcg cctcgttcct tgcgcttctg tggcgaggcc
    10321 ggctggtcat cgtgggaacg tttgttgttg tcgtcaccgt ggtcacgatc gcggtctttg
    10381 tcatgacgcc atcgtttacg gcaaaagcgt cgttcatgcc gcctccggac gacgccggaa
    10441 gcgtatcatc tttcttgcgc gacccgttga gcgccgtgct gaagcgaggt tctggcggag
    10501 ccgtgttgga tcgtctggcc agttttctga acagccagac cactaaaagg atcctggtgg
    10561 aacgattcaa cctttggcct cattacaagg cgagaaatct atccgaagcg atgattgctc
    10621 tggaaaagca gaccagggtg gtcgtaacaa cagaagggac tatcgagctt gcggttactg
    10681 accgcgaccc aagactggcc gcggcgcttg ccaactgcta tctggacgtt gtcgacagtc
    10741 tgtttcgtga cgcacaaacc agccacgctc ggcagatgcg cgaattcctg gagcgacgtc
    10801 tcaacgagaa ccgacgcgcg ctcgcggagg cggagtcaac ggcgaaggca ttcagcctca
    10861 ggtatggcgt ggtggcatta cctgaacagg ttgccagcct ggtaggtctg atggcctcta
    10921 tcgagggcaa gatcatggcg ctggacgtgc agatcggcgc ggcacggcag gtgcttggtc
    10981 cggatcattt ctccctcagg gaactgacga gtcagaggga gcaactcgcc gcgcagagga
    11041 aagcactgat cgagacgaaa gggcgaggga cttccgaccc gctgttcgcg ttccgtgaag
    11101 tgccggaaag ggctcttcag tgggctcgta tcgagcggga gatcaagata cagaccttga
    11161 tccaggaact cgttttgcaa caatacgagg tggcaaaact ggaagaacaa agggacgttt
    11221 cctcactcac gatcattgac cgagccacgc cgccggaaat ccggtcatgg ccccgcagag
    11281 ggaggattat cgcgatttcc gccgcaattg ccgtcgtctg gggaattctg ctggcgtacg
    11341 cccttgagga atacccccgg ttgcgccggc gcatccggga aatgaacgat ctccattccc
    11401 gctccgacgc ctgacggaaa ccatgccgga atgaacacgc ggagattgac gggggaagca
    11461 gctgtttaca ccttcgggat cgtccttgac accctcgctc ccttccttgt tctccccatc
    11521 ctcggtcggt acctcacccc cgggcagttc ggacttgccg acgtcattac ggccaccgca
    11581 gggttgacct acttcgcggt cgggttcaac ctcgatcagg cgctgactcg ttactactac
    11641 gacgaagagg atgcccaccg caagcgactc gtcaccacac acctcgcggt ggtatccgta
    11701 tccggcacgg tctttctcgt tctaggcctc gtgggactgc acgcattccg cggcagtctc
    11761 caggatctgt cagcctggca ctgggcgttg ttgagtgttc cccttatggt gatgagcgat
    11821 caggcagcca cactcttccg cctgcaacac caaccattca aacaactcgg gctgttgagt
    11881 gtccgcgcgt ttgtatgggt gttcttcacg tcctttttcc tcgtctggtt gcgtgccggg
    11941 atttcttcca ttttcatcgg aagggtcgcg ggggccattt tcgggttggg tgccagcgcg
    12001 tacgctcttc gccgtctgta cgcgctccgt ggcaaccttc gcttcgccgt ccgggcgctg
    12061 aggttttcac ttccgacact tcccgcggcc ttcgcgagct ggggcctggt aaacgccgct
    12121 cgatatttcc ttgtgatcgg ggcgtctgcg gaagaaacgg ggtactacgg agtcggggtt
    12181 cgcctgacgt acatcgtttc gacgatcggg atcggcgtgg tgatggcatg ggggcctttc
    12241 ttcatgggaa tcaaagaaga gcccaatgcg agagaaattc aggcgagagg tctgttgtac
    12301 ttcatcgctg cgaacgtggt ctgcatcgcg ccgcttctga tgttctcacg tgaggcagtg
    12361 cttctcctta tgggcagcca gtatcttccc gcggttcgat tggtcggtcc gatgctggcg
    12421 gccactctct tttcgaatct atcgcttttc ctgtttgtcc agatttctgt ttctgagcgg
    12481 acgcactggc aatcggtgag ctacgccgtc ggcctcgtgg tgatggtgat cgcgaacgtg
    12541 ctgctggtgc cagcgatgaa agggatggga gccgccatcg cgatgctgtg cgggcacctt
    12601 gccaccgcag gaaccatgtg gttcgcaggg cagcgcgtgt acactgtgcc ggtgtctgtc
    12661 gctcgcgtgg gtatcctcat tctcgtactg gcggggtgcc tggtgctcgc gccgacaaca
    12721 acgacaaggt ttggaacgac ccttcaggga attgcgctca gggcactcct cactgtgctg
    12781 atgtggtcgg cctgcctcgc cgcggtgggc cccggggacg tgttgaggtt tgccgcaaca
    12841 atggtgggaa agagaacatg aacgtcgcag gccgcttgtc cagcggagca gactcgttcc
    12901 accagttggc aatcgccggt gcaacgtccg ccgcactcgt cggcgcggtg ttcatgttgc
    12961 ctccggaagt atccgcgttt cttttttacg tgtctgcggg cgcaatcatt ctctgtatct
    13021 ggatcgttcg ttccgctccc cgcgaagacg tgacaggatt actcgtcgga gcgctctgtg
    13081 cgctcattct gcgcgccgct cttgcctggt gggtgtttga ccatcccgag cgctactctg
    13141 acacccgcct ctactggacg ctggggaacc ggatcgcgtc tctttgggaa cctggccgca
    13201 tccttcccgt agaaatggtg aaagcaaccg ggacaagctc ctgggggttc ttcttctggt
    13261 ccggcctgca tatttccatc ctccggaccc ccctcctgct ggtcgccgcg aacgggatcg
    13321 caggaaccgc ggtcggcgtg atggcctatc acattggtcg tcacgtctgg tcgcgcgagg
    13381 ttgggcggcg cgcagcatgg cttgtctggg catcaacctc ccagctcatc gtcgatgctc
    13441 atcttcttcg agattcgggg gcaatcctgt tcggcgccgg cgtggtgtac ggttcactgc
    13501 ttctggcgga ccgcttgagc ctgcgcggat tgctctggta cgggatcagc ttcttcctgc
    13561 tgatgcagat gcgcagttac atcgcgatgg tcgtggcgcc agcttcgctg ctcggggtcc
    13621 tcattggtgc ccgggggcga aggctctccc ttcttttgat ctacctcgtg gcggggacag
    13681 gcgcggcatt cttcctcgcc tcgacggact tcgtacgaat ccttaccgct ctcggcagga
    13741 aaaacgtcat cgccgacatg ctctacctca cgcaggtagg gctgagcggc atccgtccat
    13801 ctgcgtcgta cttctcgggt gtgcacctgt caacgccgcg ggaaatgatt gcgtttcttc
    13861 cgtttggaac catgcgggag ttgctgtcac ctctcccatg gatgccgatc gagatggaca
    13921 gatggtttat cccggaaggc ctgctccggt ggacgttgat tccgttattt gttctcgggg
    13981 ttgtggaagc tctcaggaag a
//
LOCUS       DAOSOO010000068        13965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4402_length_13965_cov_5.214091, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000068 DAOSOO010000000
VERSION     DAOSOO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4402_length_13965_cov_5.214091"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLT86_06615"
     CDS             complement(<1..150)
                     /locus_tag="PLT86_06615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOT36318.1"
                     /translation="MARLYTRSGDGGRTRLADGSAVPKDDVRVEACGTAEPSASLVLP
                     PSPDRV"
     gene            complement(171..1085)
                     /locus_tag="PLT86_06620"
     CDS             complement(171..1085)
                     /locus_tag="PLT86_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT36319.1"
                     /translation="MRIVSLLPSVTRVLYALGAGDDIVGVTYACPQPEGQFKPVVSHD
                     MFATSGYTSEQIAAFLRMLGHQAQGLFTIDEAKLEECAPDVVFLQGSCELCVPGTNSN
                     EGTLAGISGNPRAIMIQPHSLGEVMADIRRIGEVANRKPEAETFLSTLKQRMMKVMRL
                     AAGAARGKRRRVLLLEWVDPPTACGLWLPELVDAAGGIPLLGSPQRPAVPTPWEQVLS
                     ANPEVIIISPAGFGLEGAANETELLRKREGWSSISAVVSGDVYAVDGTRFFFQPDDRV
                     IDSLELIASILYPEVFGEVHRPDALRLG"
     gene            1286..2392
                     /gene="ald"
                     /locus_tag="PLT86_06625"
     CDS             1286..2392
                     /gene="ald"
                     /locus_tag="PLT86_06625"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOT36320.1"
                     /translation="MRIGVPKEIKPDESRVALLPSGVHQMVEAGHELFVETNAGRESG
                     YSDSDYQAAGATVVGHASEVFGHADLILKVKEPQQEEIRLIRQGQVVFTYFHFAADRH
                     LVDAMVLSGAVAIAYETVQTEDGELPLLIPMSEIAGRMAIQQGAKYLERRMGGSGVLL
                     SGVPGVAPANVVILGGGVVGSNAAVIAAGMGADVFILDTNLRRLRHLDEVMPPNVRTI
                     MSNHYNIRKLLPIADLVVGCVLIPGAKAPKLVTREMLSLMKPRSVIVDVAIDQGGCFE
                     TSHPTTHEDPVFVVDDIVHYAVTNMPGTVPRTSTIALTNATLPYALKIANDGWKAAAA
                     SSSAIRSGLNIVEGKVVHPAVANAFGLECAEWSP"
     gene            2467..3954
                     /locus_tag="PLT86_06630"
     CDS             2467..3954
                     /locus_tag="PLT86_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT36321.1"
                     /translation="MRDTTSSESLDIHSRSVAQRRLIAEGIFASGQENISGGVILLVY
                     ALALGANTFQIGLISTAGMLGTALQLVADRLLKIAGSRRRVGCAATALLGAGRLSIAL
                     LPYATVWIAAQYLAWGLLAGLVVISGVAQVIEVVRLSWISEVVPENERGRFLGERQLV
                     VQLIGSVIAICAAGFLDWQKGIDAARGLVVCQAYFAIAAFLAVGSIAAFLMMPEPPLR
                     IRNGNGGWHVIVAPFRDAKFFPLMVYSVTWNFAVGFAAPFFNLYLIQVLQMPVSAVAA
                     FNFASQFFSLYCVRLWGRLVDRFGSLPVLRIGVFGKGLYPVAWFLLWPTPETYSTTLL
                     YFLTACVFLFNVLNSANAVSTVSLAMRLMPKDQGTSYLATFRTFANWVHAVSPALAGI
                     ISTGLQGIGWSLQFSIFLLFAISAIGRFAALWTLRFVHEPDARKVSRVFAALKRVPGF
                     SFSHGIMPWFRFWGGPFWAGFTVVKIRMGRMVSTWRGAWPGQDNG"
     gene            3993..5237
                     /locus_tag="PLT86_06635"
     CDS             3993..5237
                     /locus_tag="PLT86_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36322.1"
                     /translation="METYVELGRAIPVMGTFDVVVVGGGCAGFAAAVASARCGAQTLV
                     IERFPYFGGTATASLMANINGFRNQVEPDSVQTVKGIPAEVIRDLHELGGLGKSPYPQ
                     KEYPIEKGTLSYSYAIDTEAFKYVTLRKAVEAGVRILFHTFFVDAIKEANSVRGVIVE
                     NKSGRQAIFARVVVDASGDADVAARAGAQFWQTKQSENKRLTDQLMYRIGGVSEASDI
                     GGCRARNGVTVWGPGSFVQDGTDAEEVTRSEISARLAVWEHFQTLKAKHTGMEAAYVA
                     ETPPLIGIRQTRFVRGLYSLTADDAISGRRFDDVVAISSCPIISYYGYRRYLEHEGFD
                     IPFRCLVPLELDGLLIAGRCISSEQEPYESFRAMAPIMAIGQAAGAAAALASKSSVKV
                     RDVEISRLQSLLVEQGAELRLA"
     gene            5288..6085
                     /locus_tag="PLT86_06640"
     CDS             5288..6085
                     /locus_tag="PLT86_06640"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOT36323.1"
                     /translation="MNHHRPKRAFERAEFLSSAEGRGIRILSEYLEPEQRLNALRIWH
                     TAVFFGSARLRDRETAARQLAELERRNAAEDEIAAARVDLEMSRYYEDARALAAMITN
                     WSSTLPEEERLYVCSGGGGGIMEAANRGASEAGGRTVALNIQLPFEQVANPYTQEELT
                     FDFHYFFMRKFWFVYRAKVIIVFPGGFGTLDELLESLTLTQTGRVEKRMPIVIYGKEF
                     WNSVINFSVLAEKRVISPSDLELFHFSDTPEDAFAHVCEKLSFDAGT"
     gene            6147..7241
                     /locus_tag="PLT86_06645"
     CDS             6147..7241
                     /locus_tag="PLT86_06645"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT36324.1"
                     /translation="MPRANSEAVRERQARAKWYAARRVDYPRKVMDLWKGVMRERFLR
                     TRELANFRGFRGDFHTHSNHSDGAGTVAELAHFRDAAGLDFLFVTDHSGLSQKRDCLR
                     FERMWWGQEPGTQHHHLGILGLTRKFVPAKDLMRDYAAIKRRGGIPFIPHPCGWFPTT
                     RYTEEQIAALDTLDDAFIMEIVNGANQIFDCWDVTDAQSVELWDRLLCAGKRVTALGN
                     TDAHLAEAIGDIWTGVLAGECTHTAILDALRMGRVFVSDAPVIDISVQCDGSEPVGMG
                     GTLFVPPSKRNSPLVVRILAADSAGLASVSLVGGGMVLESWNVADKAVLSVSFVVPTR
                     QEPVYFRCECRSTDGRRAFSNPVYVVRKTE"
     gene            7251..9173
                     /locus_tag="PLT86_06650"
     CDS             7251..9173
                     /locus_tag="PLT86_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT36325.1"
                     /translation="MRKYWNPFKPPVKRRKPIERSSRRSASFDPKNQPKRTADLLRLE
                     KEYIRPYWGRMVLAILISTVSSITPFAFGYTARFIVDRILELHRWKGSAIIHVPAAEM
                     PEKMKLLFIVLAANLGLHLLAIVCSWIFHYNAVLVAQRIVFTLRKQLYEKLQKLQITY
                     FDQRITGKIMARVLDDVSVIQGNVTSTFVSIVTNVMMFLVGVVILFSINAKLAAIAII
                     IVPTWAIVYRFFHKAIIQNHRHLREKNSEIYGVVEEKISAIRVVKAFAAERREDRRYM
                     HLAADFVRLGVRQTKLSNGLTFLASLISVFGTSLILYLGALYVRDRVITVGDLLYFHS
                     SVANLFSPVVVLTGLNATVQWVLVVLRRVFDILDEPITTSDPPNPVPLTSVRGEVEFD
                     HVSLRYPNATTNALTDINLKVPAGTTVAVMGPSGSGKSSMMNLIMRFYEPTEGHLRID
                     GIDVRDVALRDLRKHISIVPQEITIFTGTIAENIMYGRFDAKPAQVIQAAKEAELHNF
                     IMSMPEKYETYVGEHGTSLSGGQRQRLAIAMSLLTDPKILILDDSTSALDAETESRLR
                     ATLKRVMKNRTSFVITHRIATARDADMIVVLENGRIAEVGTHEELIEREGAYCRIVEL
                     QQRGAVLIEEDSPALS"
     gene            complement(9166..10071)
                     /locus_tag="PLT86_06655"
     CDS             complement(9166..10071)
                     /locus_tag="PLT86_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT36326.1"
                     /translation="MPSRFRVYVYVLVTVLTWSHAFVAIKYCLRYVSPWELLVLRHVP
                     AALVFCVYLAARRSSHELRRILRDDGVRFLLLGAAAVTGYHLSLNMGSQVIPSGTVSL
                     VIGSSPVFTLVMAALLIRERPALWQISGVVIAFAGLYACIRFGGGKDVDLRYVSGALV
                     LLLSPFFASIHTTLSRPLSRKYGALNTTAFTIILGTVPLLVGADADLVHKAAALPAGF
                     WAADLFLGLGCTAAAYLLWTKALQVMEATKVAVFLYLIPVLGIAWGWLYLDERVSWWL
                     VAGAALVIFGVVLANRKAVAPAPVN"
     gene            10235..11575
                     /locus_tag="PLT86_06660"
     CDS             10235..11575
                     /locus_tag="PLT86_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT36327.1"
                     /translation="MPRKSTRLPNIVFFGVDSLRSDHMSAYDYHRLTTPHIDRLADEG
                     VLFEKMFSPNIPTPPGYIPMLTGRDLFSTQVVSLHHKDPLDPAIPTLPEILRDFGYSS
                     ACIGFGPGLYRGFDEYAEYRAWMNWEERPGDKAGKLNEVTMPILNRMAASNKPFFLFM
                     RHMDPHSPYLPPHPYDRMFYTGDECDPNNKSMEPVFAFAPFADFFRSWMPPGITDIEY
                     VNASYDGALAYMDGCIQQLLNRLDELGIVDETIVVFTADHGETLDEHDCYFDHHGLYE
                     PTLTVPLIIRYPGRLPEGVRVPGHTQLKDIVPTLMELIGIRPKVKFDGMSQIPLLTGD
                     RATNATEFYITECTWMRKHGIRTTEWKFFEALEPDFHNKPPVELYNLVEDPLELNNLA
                     DKEPGMVKELRRRMNAWIKKRTKQTGKGNPILDYHIGTDRRIGSVATANKLQKK"
     gene            11683..12132
                     /locus_tag="PLT86_06665"
     CDS             11683..12132
                     /locus_tag="PLT86_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36328.1"
                     /translation="MLTLTSRPYLFVDDQFIDTADSVRIELHHPVRQNVAFSFDAPWE
                     GPSSTYCTVLDIAAGNDSGTCPFEQPRYRLYYRGMAGDTDLVGECTCVAESEDGIAFR
                     RRNTWKFPACGSTANNIIWAGQNDISTTSPRLSTQTPRALLRSVSKP"
     gene            12153..13049
                     /locus_tag="PLT86_06670"
     CDS             12153..13049
                     /locus_tag="PLT86_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36329.1"
                     /translation="MFALASPDGLDWHLFHDDPVLTEGDFDSLNVAFWDAANGWYAAY
                     VRKSHGGFRSIDRCISEDFIHWSKPEPLDLGPGELEHLYTNAIRPYARNASLYLGFPM
                     RFFPHRTRFDSLAEKGVSDAVLIASRDGLHFPRHFREAFVRPGLDQRNWTQRNLITAA
                     GMIQTSPSELSIYWVEHYDHHDCNLMRGTLRVDGFTSLHGGVPGGSAVSRPLKLEGTT
                     LQANLSTSAGGSVKIGLCDETGKPLEGFSIEDCDDLFGDDLARTVRWNGSPDLSRLKG
                     DTVRLHLMLRDADVYSLSAVDR"
     gene            complement(13050..13745)
                     /locus_tag="PLT86_06675"
     CDS             complement(13050..13745)
                     /locus_tag="PLT86_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT36330.1"
                     /translation="MLLRPDVIFFDYGNTLVKDTEDWFGDISRYLAPLGMELTEDRFR
                     IGKAAAEHYAGSYRQKNGTRTRLEDRFWFNYCKAFLEAAFGDGAGRLAEEMHAVQFFT
                     NEIYPDTHAVLRELRRRGYRLGVISNWEAPTLPSLFERFQLTQYFEKILPSRDAEASK
                     PDPLIFQKAMSALAVKPEDAVHVGDSFPCDVEGAQGVGITPVWLNEKGGAAPDGSSVL
                     QIRNLSGLLSLLA"
     gene            complement(13750..>13965)
                     /locus_tag="PLT86_06680"
     CDS             complement(13750..>13965)
                     /locus_tag="PLT86_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36331.1"
                     /translation="SVTNAFSVVIAYVLGNALGAFFALRGIQAKIAGADKKTSPSDDV
                     PNRVADQRKALTAFATFVSGILAMLSG"
BASE COUNT         3032 a         4034 c         4009 g         2890 t
ORIGIN      
        1 cacgcgatcc ggggacgggg ggagaaccag actggcggac ggttccgcag tgccgcacgc
       61 ctccacacga acatcgtctt tcggcactgc ggaaccgtcc gccagtctgg ttctcccccc
      121 gtccccggat cgcgtgtaaa gccttgccat attgtcctca tcatccctga ctaaccgagc
      181 cgcaacgcgt ccggccggtg tacctcgccg aaaacctcgg ggtaaagaat ggaggcgatg
      241 agctccaggc tgtcgatcac acgatcatcc ggctggaaga aaaatcgtgt gccatccacg
      301 gcatacacgt ccccggaaac tacggccgaa atggacgacc acccttctcg tttgcgcagc
      361 aactcggttt catttgccgc tccttccagc ccgaatccgg cgggcgaaat aatgatcacc
      421 tccgggttcg cgctcagcac ctgctcccac ggcgtgggta ccgccggtct ctggggtgac
      481 cccagcaggg gaatcccgcc ggcagcgtcc accagttccg gcagccagag cccgcatgcg
      541 gtgggagggt ctacccattc caacagaaga acccttctcc gttttccacg ggccgcaccc
      601 gccgcaaggc gcatgacttt catcatgcgc tgctttagtg tcgaaagaaa cgtctcagct
      661 tcgggcttcc tgttagcaac ctcgcctatt cgtcggatgt ctgccataac ctcgccgaga
      721 ctgtgtggct ggatcataat tgcccgaggg ttaccggaga tacccgccag cgtcccctcg
      781 ttcgagttgg tacccggtac gcagagttcg cacgacccct ggagaaagac aacgtccggc
      841 gcgcattcct ccagtttcgc ctcgtcaatg gtgaacaggc cctgagcctg atgccccaac
      901 atcctgagga acgcagcgat ttgttcgctc gtgtaacccg aggtcgcgaa catgtcgtgc
      961 gacacaactg gtttgaattg cccttccggt tggggacacg cgtaggtaac ccctactatg
     1021 tcatcccccg cgccaagcgc atacagaacg cgcgtgacac tgggcaataa cgatacaatg
     1081 cgcatttcat ttgctccgtg atttcggctc gaaactccaa tccagaaaga cgttcagatt
     1141 acacatcgag aaccagttct tccaagaccg gtgcgtgaaa acaggtaccg taatatattc
     1201 accattgcgc ttgcatttgg cattcaggag cacccggcaa tggtgacgaa tactgacacg
     1261 tgtttgcgga atgaggaggc gcgccatgag aattggtgtc cccaaagaga tcaaacccga
     1321 cgaaagccgg gtggcgttac ttccctccgg agtacaccag atggtggaag ccggacacga
     1381 gcttttcgtt gaaacgaatg cggggaggga aagcggctat tctgacagcg actaccaggc
     1441 ggccggcgca actgttgtag gacacgcttc cgaggttttc gggcacgcgg acctcatcct
     1501 gaaagtcaaa gagccgcagc aggaggaaat ccgcctcatc cggcaggggc aggtggtgtt
     1561 tacctacttc cacttcgccg cagaccgcca tctcgtggac gcgatggttc tcagcggcgc
     1621 agtggcaatc gcgtacgaaa cggttcaaac cgaggacggg gaactgccgc ttcttattcc
     1681 gatgagtgaa atagcaggac ggatggcaat ccagcagggc gcgaagtacc tcgagcgccg
     1741 catgggcggt agcggcgtct tgttgagtgg cgtgcccggt gtcgcgccgg caaatgttgt
     1801 cattctgggc ggcggcgttg tcggttccaa cgccgccgtg attgcggccg ggatgggagc
     1861 cgacgtgttc attctggata ccaatctccg ccggttgcga catcttgacg aggtaatgcc
     1921 accgaacgtt cgaacaataa tgtcgaatca ctacaacatt cgaaagctcc ttcccatcgc
     1981 tgacctcgtg gtggggtgcg tgctcattcc gggcgcaaag gctccgaagc ttgtcaccag
     2041 ggagatgctg tcgctcatga agccacgctc agttatcgtg gatgtggcga ttgatcaggg
     2101 gggctgtttt gagacgtccc acccgaccac acacgaggat cccgtttttg tggttgacga
     2161 catcgttcat tacgcagtga cgaacatgcc cgggaccgtg ccaaggacct ccacgatagc
     2221 cctgacaaat gcaacgttgc cgtacgccct gaaaatcgct aacgacggat ggaaagcggc
     2281 cgcagccagc agcagcgcca tccgaagcgg ccttaacatc gtggaaggca aggtggttca
     2341 tcccgcggtt gccaacgcat tcggactcga atgcgccgag tggagcccct gaaacccaac
     2401 ggtttggtca ggaccggacc cggcgtacct ttttgccaga cgaacacggt tctccggatc
     2461 tggtgaatgc gcgatacgac ttcttctgaa tctctcgaca tacattcccg ttccgtcgcg
     2521 cagcgaaggc tcatcgcaga gggtatcttc gcgtccggcc aggaaaacat ttccggcggc
     2581 gtgatccttc ttgtgtacgc ccttgcgctg ggcgcgaaca ccttccagat cggcctcatc
     2641 tctaccgccg ggatgcttgg aacggcgctg caacttgtgg cggaccgtct cctgaaaatc
     2701 gcaggcagca ggaggcgagt aggctgcgcg gcgaccgcgt tgctgggagc tggacggctt
     2761 tcgatcgcgt tgctcccgta tgcgactgta tggattgccg cacaatacct cgcatggggt
     2821 ctgcttgccg gtctggtcgt catcagcggc gttgctcagg ttatcgaagt cgtccgcctc
     2881 tcgtggattt cggaggtcgt tccggagaac gaacgaggac gattcctcgg cgaacgtcaa
     2941 ctcgtcgtgc agctgatcgg aagcgtgatc gccatttgcg ccgctggctt cctcgactgg
     3001 cagaagggaa ttgacgccgc gcgcgggttg gtcgtgtgcc aggcctattt cgcgatagct
     3061 gcgtttctgg ccgttggcag cattgcggcg tttctcatga tgcccgagcc tccgctgcga
     3121 atcagaaatg ggaacggcgg gtggcacgtc atcgttgccc cgttcagaga cgcgaagttc
     3181 ttcccgctca tggtctactc cgtgacgtgg aatttcgcgg tcggattcgc cgctccattc
     3241 ttcaacctgt acctcattca ggtgctgcag atgccggtga gcgccgtggc cgcgttcaat
     3301 ttcgcgtcgc agttcttctc cctttactgc gtgcgtttgt gggggcgcct tgtcgatcgg
     3361 ttcgggagcc ttcccgtcct gcgcattggt gtgtttggca aggggctata tcccgtcgca
     3421 tggtttcttc tctggccgac gccggagacg tactcaacca cgctcctgta ttttctgacc
     3481 gcatgcgtgt tccttttcaa tgtgctcaac tccgcgaacg ccgtttccac agtgagtctc
     3541 gcgatgaggc tcatgccgaa ggatcaggga accagttacc ttgcgacgtt ccggactttc
     3601 gccaactggg tacacgcggt cagtcctgcg ctcgccggga tcatctccac ggggctccag
     3661 ggaatcggct ggtccctgca gttcagtata ttccttctgt tcgcgatttc tgccatcggt
     3721 cggttcgctg cgctgtggac cctccggttt gtgcacgaac cggacgcaag aaaagtctcg
     3781 cgcgttttcg ctgcactgaa acgcgttcca ggtttttcct tttcgcacgg cataatgcca
     3841 tggttccgct tctggggggg acccttctgg gcgggtttca ccgtggtcaa gattcggatg
     3901 gggcgcatgg tttcgacatg gcgaggtgcg tggcccggac aggacaacgg gtaacagggt
     3961 tatgaccgtc gccagcgcgg agggaaagtg ccatggaaac ttacgttgaa ctcgggcggg
     4021 caatccccgt gatgggcacc tttgacgttg tggttgtcgg cggagggtgt gcaggtttcg
     4081 ccgcagccgt ggcgagtgca aggtgcggcg cgcagactct tgttattgag cgttttccgt
     4141 atttcggtgg gaccgctacc gcgtcgctca tggcgaatat caatggtttc cggaaccagg
     4201 tggaaccgga ttcagtgcag acggtcaaag ggattccggc tgaggttatc cgggacctgc
     4261 acgagctcgg cgggttgggc aaaagcccgt atccgcagaa ggaatacccg atagagaaag
     4321 gaacgctttc ctactcgtac gcaatagaca ccgaggcgtt caaatacgtg acgctgcgaa
     4381 aagcagtaga agcaggagtt cgtatccttt ttcacacgtt cttcgtagat gcgatcaagg
     4441 aagcgaactc cgtccgtggc gtaatagttg aaaacaagtc gggaaggcag gcgatcttcg
     4501 cgcgggtagt ggttgatgca agcggcgacg cagacgtggc ggcgcgagca ggtgcccaat
     4561 tctggcagac gaagcaatca gaaaacaaac gtctgactga ccaactgatg taccgcatcg
     4621 gcggagtctc agaggcgagc gatatcggtg gttgcagagc gcgcaacggc gttactgtgt
     4681 ggggaccggg gtcctttgtt caggatggca cggacgcaga ggaggtcacc cgttcagaga
     4741 tttcggctcg gctggccgtg tgggagcatt ttcagacgct caaagccaaa cataccggaa
     4801 tggaagcagc ctacgtcgcc gaaaccccgc cgttgattgg aatcaggcag acccggttcg
     4861 tgcgcgggtt gtattccctc acggcagatg atgccatatc agggcgcaga ttcgatgacg
     4921 tggtggccat cagttcgtgc ccgataataa gttattacgg gtacaggcgt tatcttgaac
     4981 acgaaggatt tgacattccg ttccgttgtc tggtacctct tgagctcgat gggctgctga
     5041 ttgccggccg gtgcatttcc tccgaacagg agccttacga atccttccgg gcgatggcac
     5101 cgatcatggc gatcggccag gccgccggag ccgcggctgc cctggcgtcc aagtcatccg
     5161 tcaaagtgag agacgtcgag atcagcagac ttcaatcgtt actcgtggaa cagggagcgg
     5221 aattgcggct tgcatgatcg caaccgaagg ttttcggtga agcgaacgaa caacgaagga
     5281 tcgcacgatg aaccatcatc ggccaaaaag agcattcgaa cgggctgaat tcctttccag
     5341 cgccgaaggc cgcggaatca ggattctgag cgagtacctt gaaccggagc aaaggctgaa
     5401 cgcgctgcgt atctggcaca cggcagtgtt cttcggttcc gcgagactgc gcgaccgaga
     5461 aacagccgcg aggcagcttg ccgagctgga gaggcgcaac gccgcagagg acgagatcgc
     5521 ggccgcccga gttgacctgg aaatgtcccg ttactacgag gacgctcgtg cgctggccgc
     5581 gatgatcacg aactggtcga gtacactccc ggaggaagaa cgcctgtacg tctgctccgg
     5641 cggaggcggc ggaatcatgg aagccgctaa caggggagcc tctgaggccg gtggacgaac
     5701 agtcgcgctc aacattcagc tccctttcga gcaggttgcc aacccgtaca cacaggaaga
     5761 gctgacgttc gatttccact acttcttcat gcgcaaattc tggttcgtgt accgcgcgaa
     5821 agtcataatc gtgtttcccg gaggctttgg aacgctcgac gagttgcttg aatctctcac
     5881 gttgacccag accggacggg tggaaaaacg catgcccatc gtgatttatg gcaaggaatt
     5941 ctggaacagc gtgatcaact tctcggtcct tgccgagaaa cgagtcatct cgccctccga
     6001 tctcgagctt ttccattttt ccgatacccc ggaagacgcg ttcgcgcacg tgtgcgaaaa
     6061 actcagtttc gatgccggca cctgagacga ctgaaaaccg cgtggaacgg gtgaggcaat
     6121 cgagcaccga ccgaaaggaa ggcaatatgc cccgggcgaa ctctgaagct gttcgggaac
     6181 gacaggcacg agccaagtgg tacgcagcca gacgggtgga ctacccgcgc aaagtgatgg
     6241 acctctggaa aggggtcatg cgggaacggt tcctgcggac ccgcgagctt gcgaatttcc
     6301 gaggctttcg tggtgacttc catacccact ctaaccactc cgacggggcg ggaacagtgg
     6361 ctgagctcgc gcatttccgc gatgcggccg gccttgattt tctctttgtc accgaccatt
     6421 ccggcctttc ccagaaacgc gactgcctca gatttgagcg gatgtggtgg gggcaggaac
     6481 ccggaacaca gcatcaccat ctcggcattc ttgggctgac aaggaaattc gttccggcaa
     6541 aggacctgat gcgtgattat gctgccatca agcggcgtgg cggcattcca ttcatccccc
     6601 acccgtgcgg ctggtttccc accacacgtt acaccgagga acagatcgcc gcgctggata
     6661 cgctggatga cgccttcatc atggagatcg tgaacggcgc caaccagatt ttcgactgct
     6721 gggacgtaac cgacgcacaa tcggtggagc tctgggatag gctcctgtgt gccgggaagc
     6781 gtgtcacagc gcttggcaac acggatgcgc atcttgcgga ggcgattggt gacatttgga
     6841 ctggggtgct tgccggggag tgtacgcaca cagcgattct tgacgccctg cgcatgggac
     6901 gcgtgttcgt gtccgacgcc cctgtaatcg atatctctgt tcaatgtgac ggttctgaac
     6961 ccgtcggaat ggggggaaca ctcttcgtcc ctccttcaaa gcgcaattcg ccgctggtcg
     7021 tgcggattct cgccgcagac agcgcaggac ttgcttcggt ttctctcgtg ggtggcggca
     7081 tggtcctgga atcgtggaac gtggctgaca aggccgttct gagcgtttcg ttcgttgtgc
     7141 caacccgcca ggagccggta tattttcggt gtgaatgccg gtcaaccgat gggcggcgcg
     7201 cattcagcaa ccctgtgtac gtggtaagaa aaaccgaata aagcctttcg atgcgaaaat
     7261 actggaaccc gttcaaacct ccggtcaagc gccgcaaacc tatcgaacgc tcctcgcggc
     7321 gaagcgccag cttcgacccg aaaaaccagc cgaagagaac cgccgacctt ttgcgtctgg
     7381 aaaaggagta catccggcca tactggggcc gaatggtact ggcgattctg atcagcacgg
     7441 tcagttccat cacgccgttc gcgttcggtt acaccgcgcg tttcatcgtt gacagaatcc
     7501 tcgaacttca ccggtggaag ggttctgcaa taatccatgt tccggctgcc gaaatgccgg
     7561 aaaaaatgaa actgctcttc atcgttcttg cggcgaatct tggcctgcac ctgctcgcca
     7621 tcgtatgtag ctggatattc cattacaacg cggtgcttgt agcgcaacgc atagtgttca
     7681 ctctccgaaa acagctctac gaaaaactcc agaagctgca gatcacctat ttcgaccaga
     7741 gaatcaccgg gaaaatcatg gcacgcgttc tggatgatgt gtcggtcatc caggggaatg
     7801 tcaccagcac gttcgtaagc atcgtcacca atgtcatgat gttcctcgtg ggtgttgtca
     7861 tccttttttc gatcaacgcg aaactcgcgg ccatcgcaat aatcatcgta cctacctggg
     7921 caatcgtcta tcggtttttt cacaaagcca ttatccagaa ccaccgtcat ctccgcgaaa
     7981 agaactcgga gatttacggg gtcgttgaag agaaaatcag cgcgattcgc gtggtgaaag
     8041 cgttcgcggc cgaaaggcga gaagacaggc gttatatgca ccttgccgcg gatttcgtcc
     8101 ggcttggggt gcggcagaca aagctttcga acggtctcac cttccttgca agccttatca
     8161 gcgtgttcgg cacatccctg attctgtatc ttggggcgct ttacgttcgc gaccgcgtga
     8221 taaccgtggg agaccttctg tatttccaca gttcggttgc caatcttttc agccccgttg
     8281 tcgtcctgac agggctcaac gcaaccgtcc agtgggtgct ggttgtgctg aggagagtgt
     8341 ttgatatcct cgatgaacca attaccacct ctgatccccc caatcccgtc ccgctgacca
     8401 gtgtccgcgg tgaggtcgaa ttcgatcacg tgagcctgcg ctatcccaac gccaccacaa
     8461 atgcgctcac agacatcaat ctgaaggttc ccgcaggtac cactgttgcc gttatgggcc
     8521 cgtccgggtc tggcaagagc tccatgatga acctgataat gcggttctat gaaccgactg
     8581 aaggacacct gcgcattgac ggtatcgacg tgcgggacgt tgcgttgcgt gacctgcgaa
     8641 agcatatcag tatcgttcca caggagatca ccattttcac aggtacgatc gctgaaaaca
     8701 tcatgtacgg caggtttgat gcgaagccgg cgcaggtcat acaggcggcg aaagaggctg
     8761 aacttcacaa cttcatcatg tcaatgccgg agaagtacga aacatacgtc ggagaacacg
     8821 gaaccagcct ttcgggaggg cagaggcagc gccttgcgat cgccatgtcg ctgctgacgg
     8881 acccgaagat cctcattctc gacgacagca cgtcggcgct ggacgcggaa actgaaagcc
     8941 gccttcgagc cacgctgaaa cgcgtcatga agaaccgcac gtcgtttgtg atcacgcacc
     9001 gcatcgccac tgcgcgcgac gcagacatga tcgtggtcct ggaaaacggc agaatcgctg
     9061 aagtgggaac acacgaggag ttgatcgaac gggaaggcgc ttattgccga attgttgagc
     9121 tgcaacaacg cggggccgta ctgatcgagg aggactctcc tgctctcagt tgaccggcgc
     9181 cggcgcgacg gcctttcgat ttgccagaac gactccgaat attaccagcg ctgcgcccgc
     9241 caccagccac cacgatacgc gctcatccag gtacaaccat ccccaggcga tgccaagcac
     9301 gggaataaga tacaggaaca cggctacttt cgtcgcttcc atcacctgca gcgccttcgt
     9361 ccagagaaga tacgcggcgg cggtacaccc caggccaaga aaaaggtctg cggcccagaa
     9421 tcctgccggg agcgcggcag ctttgtggac cagatctgcg tccgccccca cgaggagagg
     9481 cacggtgcca agaataatgg tgaacgcggt ggtgttcaac gcgccatact tccgggaaag
     9541 cgggcgactc agtgtggtat gtattgacgc aaagaagggg gaaaggagga gaaccagtgc
     9601 gccggaaaca tacctcagat caacgtcctt gcctcccccg aaacggatgc acgcgtacaa
     9661 gcccgcaaaa gcgataacta cgccggaaat ctgccacaag gcgggacgtt cacgaatgag
     9721 cagcgccgcc atgaccagcg tgaacaccgg agaggacccg atgaccaggc tcactgttcc
     9781 gctgggtatg acttgcgaac ccatgttgag ggagagatgg tagccagtta ccgcggcggc
     9841 gccaagcaaa aggaaccgca cgccgtcgtc ccgcagaatc cggcgtagtt catgtgagga
     9901 cctgcgggca gcgagataca cgcagaacac aagagctgcc ggcacgtgcc ggagcaccag
     9961 aagctcccac gggctgacgt aacgaaggca atacttgatc gccacgaacg catgcgacca
    10021 tgtaagaact gtcacaagga cgtagacgta tacgcgaaaa cgggaaggca ttcgggggtt
    10081 cccggcgggt tgtcgtacgg cgaacaaatc tgtttgaaaa tctacggagc tccgctggtg
    10141 tccccaaatg cttctcgggt cgcttgcact gacccgtttg cgctgcgata cttgacaaga
    10201 atagttctca cacccgcatt ggaaggagat tgccatgcct cggaagagca cacggctgcc
    10261 taacatcgtc tttttcggtg tggacagcct gcggtctgat cacatgagtg cgtacgatta
    10321 ccatcgtttg accacgcccc acatcgatcg gctcgccgac gagggtgttc tcttcgagaa
    10381 gatgttcagc ccgaacatcc ctactccgcc gggttacatc ccgatgctta cggggagaga
    10441 cctgttcagt acgcaggtgg tatcgctgca tcacaaggac ccgttggacc ccgccatccc
    10501 aaccctgccg gagatcctca gggatttcgg gtattcatcc gcctgtatcg gtttcggacc
    10561 tggtctttat cgcgggttcg atgaatacgc ggaatacaga gcatggatga actgggaaga
    10621 gcgccccgga gacaaggcag gaaaactcaa cgaagtgacc atgccgatcc tcaaccgcat
    10681 ggcggcctcg aacaagccgt tcttcctttt catgcgccat atggatccgc attcgccgta
    10741 tctcccgccc catccgtatg accgcatgtt ctacacggga gacgaatgcg acccgaacaa
    10801 caagagcatg gaaccggtat tcgcgttcgc gccgtttgcg gactttttcc gttcgtggat
    10861 gccacccggc atcacagaca tcgaatacgt caatgcgagc tacgacggag cgctggcgta
    10921 tatggacggg tgcatccagc agttgttgaa ccgtcttgac gaactcggga tcgtcgacga
    10981 aacgatagtg gtattcaccg cggaccacgg agagactctc gacgagcatg attgctactt
    11041 cgaccatcac ggtctgtacg agccgactct cacagtccct cttatcatcc ggtacccagg
    11101 ccgtcttccc gaaggcgtgc gcgtgcccgg gcacacacaa ttgaaagaca tcgttccgac
    11161 gctgatggaa ttgatcggaa tccgtcccaa agtcaaattc gacgggatga gccagatccc
    11221 cctcctcacg ggagaccgcg cgacgaacgc cacggagttt tacatcaccg aatgcacctg
    11281 gatgcgcaag cacggcattc gtacaaccga atggaagttc ttcgaagcac tggaaccgga
    11341 ttttcacaac aaaccgcctg tggagcttta caatctggtg gaagacccgc ttgaattgaa
    11401 caatctcgcg gacaaagagc ccggcatggt gaaggaactt cggcgccgca tgaatgcatg
    11461 gatcaagaag cggacgaagc aaacgggaaa aggtaatccg attctcgact accacatcgg
    11521 tacagaccga agaatcggtt ccgtggccac tgcaaacaaa cttcagaaga aataggcgag
    11581 aaaccacgca tccgggaggc gtctgcggac gtctcccgga gttggtaacc acgcgctaca
    11641 cccctttcgt acgtattcct tgcctttcaa agggacccga ccgtgcttac tttgacctcc
    11701 cggccgtatc tgttcgtcga tgaccaattc attgacacgg cagattcggt gcgcatcgaa
    11761 ctccatcatc ccgtccgcca gaatgtggcg ttttcattcg acgccccgtg ggagggtccg
    11821 agcagcacct actgcaccgt tctggacatc gctgccggaa atgattccgg gacctgcccg
    11881 ttcgaacagc cgcggtaccg tctgtactat cgcggtatgg ctggagacac ggaccttgtg
    11941 ggcgaatgca cgtgcgtggc cgaaagcgaa gacgggatag cattccgccg gcgaaacacc
    12001 tggaagttcc ccgcctgcgg atcgaccgcg aataacatca tctgggccgg acaaaacgac
    12061 atctccacaa cttcgccccg tttgtcgaca caaacccctc gtgcactcct gaggtccgtt
    12121 tcaaagccat aggaggggtt tcccgtcaca aggtgttcgc gctggcgtcc cccgacggac
    12181 tcgactggca cctgttccat gacgatcccg tgcttacgga aggcgatttc gattccctga
    12241 atgtggcttt ctgggacgct gcaaacgggt ggtacgcggc atacgtgcga aaatcgcatg
    12301 gaggatttcg gtcaatcgat cgctgcattt cggaggattt catccactgg agcaagcctg
    12361 agccgctgga tctggggccg ggggaactcg aacacctgta cacaaatgcc atccgacctt
    12421 acgcgagaaa tgcgagtcta tatcttggtt ttccgatgcg cttctttcca cacaggaccc
    12481 ggttcgattc tttggcagag aagggcgtca gcgacgccgt tcttatcgcc agccgcgacg
    12541 gattgcattt cccgagacat ttccgcgagg cgtttgtccg gccgggtctg gaccagcgca
    12601 attggactca acgcaacctg ataacagccg ccggcatgat ccagacgtca ccctcagagc
    12661 tctcgatcta ttgggtggag cattacgatc atcatgattg caacctcatg cgcggcacgc
    12721 tgcgcgtgga tggtttcact tcgctgcacg gcggtgttcc cggcgggtca gcggtctcca
    12781 ggccgctcaa actggaaggc accacacttc aggccaatct ctccacatcc gcgggtggca
    12841 gcgtgaaaat cggcctgtgc gacgaaaccg ggaaaccgtt ggaagggttt tcgatcgaag
    12901 actgcgatga cttgttcggc gacgatctgg cccgaaccgt ccgttggaac gggagccccg
    12961 acctttcacg cctgaaaggt gacacggtcc gccttcacct catgttgaga gacgcggacg
    13021 tgtattccct ttcggctgtc gacaggtaat cacgcgagaa gagagaggag gccggacagg
    13081 ttccgaatct gaagaaccga tgacccgtcc ggcgccgcgc cacccttctc gttaagccag
    13141 acaggggtaa tccctactcc ttgcgcaccc tccacatcac aggggaagct atcccccacg
    13201 tgcaccgcat cctcgggttt cacggcaaga gcagacatcg ccttctggaa aatcagcggg
    13261 tcgggtttgg atgcttccgc atctcgggaa gggagaatct tttcaaaata ctgcgttaac
    13321 tggaaccgtt cgaacagaga gggcagcgtt ggggcttccc agttggagat cacaccgagg
    13381 cgatatcctc ttcgacgcaa ttcacgtaac acggcgtggg tatcggggta gatctcgtta
    13441 gtgaagaact gaaccgcgtg catttcctct gccagccgtc cagctccatc gccgaaagcg
    13501 gcttcaagaa acgccttgca gtaattgaac cagaaacgat cttcaagcct cgtgcgcgtt
    13561 ccgttcttct ggcggtaact gcccgcgtag tgctccgcgg cagccttccc aatccggaac
    13621 cggtcttccg tcagctccat tcccaggggg gccaggtagc gcgaaatatc cccgaaccag
    13681 tcttccgtat ccttgacgag agtattgccg taatcaaaaa agatcacatc aggccgcaga
    13741 agcatggagt caccccgaaa gcatcgccag aatcccggac acaaacgtcg cgaacgcagt
    13801 taatgccttc ctttgatccg ccacccgatt gggcacgtca tcggacggac tcgtcttttt
    13861 gtccgcaccc gcgattttgg cttggatccc tcgaagggcg aagaaggcgc cgagcgcgtt
    13921 tccgaggaca tacgcaatca ccacggagaa cgcgttggtg accga
//
LOCUS       DAOSOO010000069        13869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4442_length_13869_cov_6.204575, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000069 DAOSOO010000000
VERSION     DAOSOO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4442_length_13869_cov_6.204575"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLT86_06685"
     CDS             complement(<1..882)
                     /locus_tag="PLT86_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT36332.1"
                     /translation="MQRRRTILIIADGTRPDVLGELIAAGELPAIAEAFPEPSMRRSA
                     VSVFPSTTGPAFLPFLTGMYPGDLNMPGIRWFDRGAWAERNGDPPFRSYVGIESYRMN
                     ADFRRDVPTLFDILPRSASIFSSVNRGVPFRGNMTGLSRAWWWLFAHWTDRWDVVGVR
                     ARKRLLASLEMDPEFVFCLIPDVDNYSHLSHCRSERAIAAYRRLDRTIGELFRELERR
                     RWREDTLVVMVADHGHSAVHTHLGLPEWMAGRGFKPFYYPRIWNRTFDSAVMVSGNGM
                     AHIHLRRGNTWTPERVQE"
     gene            complement(928..1344)
                     /locus_tag="PLT86_06690"
     CDS             complement(928..1344)
                     /locus_tag="PLT86_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36333.1"
                     /translation="MTFRARILFAVVAVFAACVVPHAEPYADSSRVSPCNARTAFALG
                     LVVPGGGQFYLGDRAGGIQAVITDVILFEALRRNDATISGVVFFIGAVHVVEALFATR
                     ECTLRNAAMRPRVTEAFPERREAPAIGLAAAFPISR"
     gene            complement(1495..2619)
                     /locus_tag="PLT86_06695"
     CDS             complement(1495..2619)
                     /locus_tag="PLT86_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT36334.1"
                     /translation="MRRILEIVRKEFAQLRRDRRLLGILIVAPVFQLFILGYAATTDL
                     REVPVAVCDMDRSASSRSLLDAVSASGDFRLRYFVDAPQELDVYLDRNKADLALVIPR
                     DYGRRVIRGEAATVQVLVDGTRTNAGLALSQLSGAIGQHAQFILLDRLTKRGISGERP
                     EVQLATRVWYNPELKSRNFMLPGVLALVLLIIATAATSMAIVREKENGTIEQIIVTPI
                     RSLELIAGKLIPFAIVGMVEVLLVLSVATLWFRVPLKGSGVLLLTLTFLFVITTQGMG
                     LFVSTVSRTQQQAMMIIIFFIALPMILLSGFVFPIENMPKPIQPITYLMPLRYFLVIV
                     RGIFLKGVGMEVLWPRVVALAAFAFGILALAVARFQKRVG"
     gene            complement(2616..3761)
                     /locus_tag="PLT86_06700"
     CDS             complement(2616..3761)
                     /locus_tag="PLT86_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT36335.1"
                     /translation="MNRARLWRLYGVIVKEFRQMRRDMISLSVLLLVPALLLALYGYA
                     LTFDVKHIRVVVLDHDKGTFSRQFTEGLFQSEYFDFAGTIDREAEATRWLDTGDALAV
                     LVFPPDFGKALRDGRTATAQVLIDGSNANTASIALAYFTAKAREFSAAQLQEFTLARG
                     IAQPAMPVVIEPRVWYNAELKSSQLLVPGLIGYLLMLAGTVATSLSVVREKERGTMEQ
                     LAVSPLGSGEFILGKTIPYLAVSLLTSGLVIAAAMVLFEMPLRGSIPWLFVTTFVFLL
                     GALGLGLWVSTLVDTQQLALQISLIITLLPALLLSGMVFPIASMPRALQWVSAIVPPR
                     HFLVIMRSILLKGTGPKAWLAELGYLTAFAVFILGIAVVRTRRRSEA"
     gene            complement(3758..4729)
                     /locus_tag="PLT86_06705"
     CDS             complement(3758..4729)
                     /locus_tag="PLT86_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT36336.1"
                     /translation="MAEGSAPIIETRDLTRKFGQFVAVDRVTLRVNRGEIFGFLGPNG
                     AGKSTTIRMLCGILAPTSGTALVGGFDVAREPDRVKEHIGYMSQRFSLYPDLTVAENI
                     RFYGGIYGLSAQQLRERGAWAVEMAGLKGREDHLTRELAGGWKQRLALGCAILHEPPI
                     LFLDEPTSGVDPISRRRFWDLIYELAGHGTTVFVTTHFLDEAEHCDRLVLIYRGRVIA
                     LGTPEELKHTAIRGTILSVRCREANNAVELLREVDGVRRVALFGASVHVITDTPKETS
                     AAIIARLGEAGTGIDGIAEVPPSLEDVFIALIEQADAEAPTAEVRGR"
     gene            complement(4744..6147)
                     /locus_tag="PLT86_06710"
     CDS             complement(4744..6147)
                     /locus_tag="PLT86_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT36337.1"
                     /translation="MRRTRWYGVSLAAIGTGLGLWSSGVAQTEAAGNALRLTVARARE
                     LAMTQNAGIRASAQSVISATAQKNEARAARLPGVSLSARYARLSDVPDPVIMTPSLTI
                     PTGPGTSITTPSTQVSLGPSVLNSYNLTATAQVPLFTGFRLENREKAAEYAAKAAGED
                     FAASQSEVGNRVEQVYWRLHSARATERVISESVRLAEAHLEDVRRLHAAGMATADDIL
                     AVQVRLAEAKLRQIQAENASELVRATLCNLLSVPVTTEIALVDSPSVVLPAIPEQDAL
                     VSSALANRGELRALAFRKQAMERGTAVAEGGKLPSVMFQTNYEVSNPNQRYFPTRDAW
                     HATWNAGIGVTWTAWDWGMVGQQAARARAEERQVDERLNQVRDAITLQVVQARLAVIE
                     GNRRVAVAEEGVRSAEAYHRNMRARYSAGTATNTEVLDAQILLERARLDLVQARTDQR
                     TAAANLAYVVGAERTED"
     gene            complement(6144..7133)
                     /locus_tag="PLT86_06715"
     CDS             complement(6144..7133)
                     /locus_tag="PLT86_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT36338.1"
                     /translation="MTDHVARAGGTMPAAALETRELTRFFGTTHAVNGVSFSVGRGEM
                     LALVGPDGAGKTTTIRMLTGVLKPTSGTATVLGHDLVRERDAVKRSIGYLSQVFSLYG
                     DLTVDENIEFFAQIHGVRRFQKRREELLSFTRLAPFRRRLAGNLSGGMKQKLALACTL
                     IHSPGLIFLDEPTTGVDPVSRRDFWIILSDLLREGITVVLATPYLDEAERCHTVALMH
                     EGRIIRRDTPAAIVETFGKRLLEVVCEDARAAYRAMKQAPEWEEAQMFGSRIHVPVED
                     VVAGTQRLQEALEEMQFRVASIREIPPSLEDAFISEIRHSEGPVNPSEGGTAA"
     gene            complement(7130..8116)
                     /locus_tag="PLT86_06720"
     CDS             complement(7130..8116)
                     /locus_tag="PLT86_06720"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT36339.1"
                     /translation="MGKMAWVAPVLAIGLGACSRGASDGDITASGVIECTEINIATKV
                     PGILREVLPREGDLLDSGQVVAVIDHSDLDWQKSQAEAQLRVARANLQLALNGPQRED
                     IAQAEAAEQQASVALEAARLDLERVSQLAATGTAPQKQLDDARTRVRSAEAAQAMARA
                     TLAKLRAGTRSEQIAAARAQVEQAEAALSAVSQRIADCVVRSPRKGYVTQRIAEPGEM
                     VPSGGTIVTLSELDPVWLKVYLTEVELAKVRLGGTAEVTLDGEPDHPRSGRVSWISPK
                     AEFTPKNVQTKEDRVKLVFAVKISLQNPDGFFKPGLPADAAFRAGDGAGAPQ"
     gene            complement(8095..8778)
                     /locus_tag="PLT86_06725"
     CDS             complement(8095..8778)
                     /locus_tag="PLT86_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT36340.1"
                     /translation="MNRDSDARQRILDAATEVFVDKGLSGARMQEIADRAQINKAMLH
                     YYFTSKEKLYETVVSAAVQGIMERVQGVLKGEDVPTEKRFLMLVDTYLDSLSKKPHVP
                     RLIMMDLLAGGEIVMRALPQAQHRASFVGAKPALDLIRRGIHEGVIHEIDPKHTLISV
                     LGMVAFSFLAEPLLSSMLQIEDRKAGQFFADRRRNVMEILQHGVLKEQDSTRVSTIPR
                     EDKWEKWLG"
     gene            complement(8887..9507)
                     /locus_tag="PLT86_06730"
     CDS             complement(8887..9507)
                     /locus_tag="PLT86_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36341.1"
                     /translation="MRMEMKLFAALTGASIALAAGILSWWSAAILSILRWVPLTDSTI
                     AWAACSGLVAGILLAWVWYRRNAGRVFSIPMGHLAVLYVIWSVFSFAFFMGFPFGAFL
                     LGCLAAVYVGRRAAHERHGGECGVRKQVRFIGFVLGVECLAAGFFGALGDPHTWRVVL
                     SLFGAEMLAERVILSLLTVIGVCAVLVSVQIAVAGWIAGRASRWGS"
     gene            9737..11557
                     /gene="guaA"
                     /locus_tag="PLT86_06735"
     CDS             9737..11557
                     /gene="guaA"
                     /locus_tag="PLT86_06735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT36342.1"
                     /translation="MNVPVQERIVVLDFGGQYAHLIANRVRRLRVFSEIMLPSEPPAN
                     LEGVRGLILSGGPSSVHSPDQPPFDARWLHTGLPVLGLCYGHQLLAKELGGEVRRGDR
                     QEYGIAELRIADHDAIFEGLEPVERVWMSHGDSVTRVPDGFAVLGGTADCPIAAMGDV
                     QRRMFGFQFHPEVTHSPHGQKMLDNFLSLCGCARTWTMEQFITQAEERIRRQAGDRNV
                     FMLVSGGVDSSVAFVLLSRALGEHRVIGLHIDNGFMRLNESVLVKESLECAGFHNLHV
                     VDASEKFLGAVKGVTDPQQKRQIVGDTFISVQQEELERIGLDPDHWMLGQGTLYPDII
                     ESGGTRHASVIKTHHNRVAIIRQMLEEGKIIEPLDQLYKDEVRQVGRQLGLPDALVER
                     HPFPGPGLSVRCLCSDGVEEPIEASAKEKVRALAGSLGFESEILPVRSVGVQGDERTY
                     RHPVALWRPDGGAVNWEELERLSTRITNTVPEVNRVMLLVYPRSLPRLKLRARFLDRP
                     RLDLLRLADHAAMSVLVEDGLAGDFFQFPTILIPLGEREEGESVVLRPLESTDVMTAS
                     FAILPERSLRRLVSRLAALPVVDAVFYDITHKPPATMEWE"
     gene            11654..11971
                     /locus_tag="PLT86_06740"
     CDS             11654..11971
                     /locus_tag="PLT86_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36343.1"
                     /translation="MYHAAKAEFALTGATARLYEIFLDATRGSAAVGDVNRALFETGL
                     VHHALMLLALGVVPEERAKGARALIDEIGKTTIMKDSFDQAREYWERVAKVNPSAPES
                     SDG"
     gene            11964..13019
                     /locus_tag="PLT86_06745"
     CDS             11964..13019
                     /locus_tag="PLT86_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT36344.1"
                     /translation="MDEAVSAALRANGYLWDLYTRREEYEPIFLDGLGRFRFFASQQR
                     APFDPVVSRFLFERGAFPAFPAGRRYAICLSHDIDALHLNRYRSGGAGLLSRARRASA
                     RSAELLLSRSLKRGDPGWNLARIADIEKERGAKSAFYSLALEEGEKDFYFRVQEVGAQ
                     LRSLVARGWEVGLHGGHGAYCDPSVLRREKIRLEVELGTPVTGFRAHYLRFDVPGTWR
                     LLAEAGFRYDTTFGYADCVGFRNGMCHPYTPFDRNADRFIPILEVPLAVMDTTLQLYM
                     RLDPADAWTVIRRLLDAVAECSGVLTVLWHPQNMNGAWGDLYLRILDYGTDTDAWLTG
                     PGELAGWWLSQQAGGNA"
     gene            13073..>13869
                     /locus_tag="PLT86_06750"
     CDS             13073..>13869
                     /locus_tag="PLT86_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HOT36345.1"
                     /translation="MVLVDKGAPCSVVVSGAQPTWLERHAAEELVHYLRLISGAALPT
                     VSGIPDGMNAVVIGRADTNSVVAEAVHEGVLSLSEDSPGWDGFVIKTARRGNRDVCFV
                     GGSSDRGTLYAVYWLLEEVLGVGFFRDGEYIPRSDSVMLPESLHIVERPVFEHREDGL
                     LAYSAKFWGWDEWKRELDWKAKRRANITWPFSGTGEITQAVLAEWGLNAAPPEPPAEP
                     TLTDRLLDYAHKLGIRVPCHLPSGSMPAVFWEAFPNARKLVMQWSEYA"
BASE COUNT         2724 a         4589 c         4000 g         2556 t
ORIGIN      
        1 ttcctgcacg cgttcaggcg tccatgtgtt cccccgccgc aggtggatat gcgccatccc
       61 gttgcccgac accatcacgg ccgaatcaaa ggtgcggttc cagatgcgcg ggtagtaaaa
      121 cggcttgaat ccccgcccgg ccatccactc cggcagcccc agatgcgtgt gaaccgccga
      181 gtgcccgtgg tcggccacca tcaccaccag cgtatcctct cgccagcgtc gccgttccag
      241 ctcccggaaa agctccccga tcgtccgatc cagccgccgg tacgccgcaa tcgcccgctc
      301 cgaccggcag tgcgaaaggt gcgagtaatt gtccacgtcc ggaatcaggc agaacacgaa
      361 ttccggatcc atctccagcg acgcaagcag ccgcttcctc gcgcgcacgc ccaccacatc
      421 ccagcggtcg gtccagtgcg cgaacaacca ccaccacgcc cgcgataggc cggtcatgtt
      481 tccccggaac ggaacacccc ggttcaccga actgaagatg ctcgcggaac gaggcagaat
      541 atcaaaaagc gtcggcacgt cccgacggaa atccgcgttc atccggtagc tctcgatccc
      601 aacgtagctc cggaacggcg gatcaccgtt ccgctcagcc cacgcgccgc ggtcaaacca
      661 gcggataccc ggcatgttca aatcgcccgg gtacattccg gtcaggaacg ggagaaacgc
      721 cggacccgtc gtggaaggaa acacgctcac cgccgaacgg cgcattgacg gctcggggaa
      781 cgcctccgcg atcgccggaa gttccccggc cgcgatcaac tccccgagca cgtccgggcg
      841 cgtcccgtcc gcgataatca atatcgttcg tctgcgttgc attctgatag gttctttcta
      901 cagggcacct gcgcgccaga aggcagatca gcgggaaatc gggaacgccg ccgccagccc
      961 gatcgccgga gcttcgcgcc gctccgggaa ggcctccgta accctgggcc gcatagcggc
     1021 gtttctcagt gtgcactccc gcgtggcaaa cagcgcttca acaacgtgta ccgcgccgat
     1081 gaagaaaaca accccggaaa tcgtggcgtc gttcctccga agcgcttcaa acaggatcac
     1141 atccgtaatg acggcctgaa tcccccccgc gcggtcgcca aggtaaaact gccctccccc
     1201 cggtaccaca agaccgagcg cgaacgcggt ccgcgcgttg cacggagaaa cacggctgga
     1261 atccgcatac ggttccgcat gcggaaccac gcacgccgca aacaccgcca caacggcaaa
     1321 caggatgcgc gctcgaaagg tcattcacgg ctccggagag gcttcagtcc ggtcactcgt
     1381 tcaggaaagg aaaaagtaaa agggaacaat gaagaggcgc tataacgaca atgaagattc
     1441 gttcatgttc caccaccgca ccggtctcaa caccccgacc acgggccgtc cgtcctaccc
     1501 cacccgcttc tggaatcgtg ccaccgcaag cgccagtatc ccgaacgcga acgccgccag
     1561 cgctaccacc cgcggccaca gcacctccat ccccacgccc ttcaggaata tcccccgcac
     1621 aatgacgagg aaatacctca gcggcatcag atacgtaatc ggctgtatcg gctttggcat
     1681 gttctcaatc gggaacacga aaccggagag cagaatcatc ggcagcgcaa tgaaaaagat
     1741 gatgatcatc atcgcctgtt gctgcgtccg cgaaaccgtg gatacgaaca gacccatgcc
     1801 ctgagtcgtg atgacgaaaa ggaacgtcag cgtcagcagg agcaccccgc tccccttcaa
     1861 cggcacccga aaccacagcg tcgctaccga aagcaccagc agaacctcca ccatcccgac
     1921 aatcgcgaac gggatcaatt ttcccgcgat caattccagc gaacggatcg gcgtcacgat
     1981 gatctgttca atcgtcccgt tttccttttc ccgcacgatc gccatggatg tcgccgccgt
     2041 cgcaataatc aggagcacaa gcgccagcac ccccgggagc atgaaattcc ggctcttcaa
     2101 ctccggatta taccagaccc gggtcgccag ttgaacctcc gggcgctcgc cggaaatacc
     2161 gcgcttcgtc aggcggtcaa gcagtatgaa ttgcgcgtgc tggccgattg cacccgaaag
     2221 ctggctcagc gcaagccccg cgttcgttcg cgttccatct accagaacct gcaccgtcgc
     2281 ggcttctccc cggataaccc ttcgtccgta gtcccgcgga atgacaagcg cgaggtccgc
     2341 cttgttgcgg tcgagataca catccagttc ctgcggcgcg tccacgaaat accgcaggcg
     2401 gaaatccccg ctcgcgctca ctgcgtccag aaggctcctg ctggacgccg acctgtccat
     2461 gtcgcacacc gcaacgggaa cctcccgcag atccgtcgtc gcggcgtacc cgaggatgaa
     2521 aagctggaat accggcgcaa caatgaggat ccccagcaac cgccggtcgc gccggagttg
     2581 tgcaaattcc ttccgcacga tttccaggat gcgtctcatg cctcgctccg gcgccgggtg
     2641 cgaaccaccg cgattccgag aatgaacacg gcgaaagcgg tcagataacc cagctcggca
     2701 agccacgcct tcggtcccgt ccctttgagc aaaatgcttc gcatgatcac cagaaaatgc
     2761 cgcggcggca caatcgcgga aacccactga agcgcacggg gcatgctggc gatggggaac
     2821 accattccgg aaagcagcag tgcgggcaaa agggtgatga tcaggctgat ctgcaacgcg
     2881 agctgctgcg tgtctacaag cgtcgaaacc cacagcccca gacccagcgc gcccagcaga
     2941 aacacgaacg tcgtcacaaa cagccacggg atgctgccgc gcaacggcat ctcgaacagc
     3001 accatcgccg ctgcgatgac gagtccgctg gtcaaaaggg atacggcgag atagggaatc
     3061 gtcttcccca gaatgaattc acctgatccg agaggactta ccgccaactg ctccatcgtc
     3121 ccccgttcct tttctcgaac caccgaaagg gaagtcgcca ccgttcccgc aagcatcaga
     3181 aggtagccta tcaagccagg caccagaagc tgcgagctct tcagttccgc gttgtaccac
     3241 acgcgtggct cgataaccac cggcatcgcc ggttgcgcaa ttcccctcgc aagagtgaac
     3301 tcctgcaact gcgcggccga aaactcccgc gcctttgccg tgaagtacgc aagcgcaata
     3361 cttgcggtgt tcgcgttcga accgtcaatc agcacctgcg cagtcgcggt tcgcccgtcc
     3421 cgcagcgctt tcccgaaatc cggcggaaaa accagcacgg ccagcgcatc gcccgtgtcc
     3481 agccaccgtg tggcttctgc ctcccggtca atcgtccccg cgaaatcgaa atactccgac
     3541 tggaaaagcc cctccgtgaa ctgtctgctg aacgtccctt tgtcatggtc cagaacaacc
     3601 acgcgtatgt gcttcacgtc aaacgtcagg gcatacccgt acagtgccag aagaagagca
     3661 ggaaccagaa gcagcaccga aagactgatc atgtcccgcc gcatctgacg gaattccttc
     3721 acaataacgc cgtacaaccg ccacagccgt gcccggttca tctcccgcgc acctccgccg
     3781 tcggagcttc cgcgtccgcc tgctcgataa gcgcgataaa cacgtcctcc agcgagggcg
     3841 gcacctccgc aatcccgtca atccccgtcc cggcctcacc gagccgcgca atgatcgccg
     3901 ccgaggtctc cttcggcgtg tcagtaatca cgtgcaccga cgccccgaac agcgccaccc
     3961 gccgcacgcc gtccacctcc cggagaagct ccaccgcgtt gttcgcctcc ctgcaccgca
     4021 cggaaagaat ggttccccgg atcgccgtgt gcttcagctc ttccggcgtc cccagcgcga
     4081 taacgcggcc ccggtagatc agcaccagac ggtcgcaatg ttccgcctca tcaagaaagt
     4141 gggtcgtcac gaacaccgtc gtgccatgtc ccgcaagttc gtagatcagg tcccagaacc
     4201 gacggcggga aatcgggtcc acccccgacg tcggctcgtc cagaaacagt atcggcggct
     4261 cgtgcagtat cgcgcatccg agcgcaaggc gctgcttcca cccccctgca agctctcgcg
     4321 tcaggtggtc ttcccggccc ttaagaccgg ccatttccac cgcccacgcc cctcgctccc
     4381 gcaattgctg cgccgaaagc ccgtagatgc ctccataaaa acggatattt tccgcaaccg
     4441 tcaggtccgg atagagcgaa aaccgctgcg acatgtagcc gatgtgctcc ttcacccggt
     4501 caggctcacg tgcaacatcg aaccctccca ccagcgctgt tcccgaagtg ggcgcaagaa
     4561 tcccgcagag catccggatt gtcgtggact tgcccgcccc gttcggaccc agaaatccga
     4621 atatctcgcc cctgttcacg cgcagcgtca cccggtcaac ggcaacgaat tgcccgaatt
     4681 tccgcgtgag atcgcgcgtt tctattatcg gcgcggaccc ttcagccatc gtgactcccg
     4741 ccttcagtcc tctgtccgct cggcgccgac cacgtatgca aggttggccg ccgccgtcct
     4801 ctggtccgtc cgtgcctgta ccagatcaag ccgcgcccgt tccagcagaa tctgcgcatc
     4861 cagaacctcc gtgttcgtcg ccgtcccggc ggagtaccgc gcacgcatgt tccgatgata
     4921 tgcctcggcg ctccgcactc cttcctcggc aactgccacc cggcggttcc cctcgattac
     4981 cgcaagacgc gcctgcacca cctgcaacgt gatggcgtcg cgcacctggt tcaatcgctc
     5041 gtccacctgc cgctcttcgg cgcgcgcccg cgccgcctgc tgcccgacca taccccagtc
     5101 ccaggccgtc cacgtcactc ctataccggc gttccatgtg gcgtgccagg catcccgggt
     5161 cggaaagtag cgctgattcg ggtttgatac ctcgtagttt gtctgaaaca tcaccgaagg
     5221 aagttttccc ccctccgcaa ccgccgtccc acgctccatc gcctgcttcc ggaacgccag
     5281 cgcgcgaagc tccccgcggt tcgccagcgc cgacgatacc agcgcgtcct gttctggaat
     5341 ggccggcaaa accaccgacg gcgagtccac cagggcaatc tccgtggtta ccggaaccga
     5401 aagcagattg cacagcgtgg cgcgaaccag ctcggacgcg ttctccgcct gaatctgccg
     5461 cagtttcgcc tccgccagcc gaacctggac cgccagaatg tcgtctgccg tcgccatccc
     5521 ggctgcgtgc agccgtcgca catcctcaag atgagcctcg gcgagccgta ccgactccga
     5581 aatcacgcgc tccgtcgccc gcgccgaatg cagccgccag tacacctgct ccacccggtt
     5641 cccgacttcg gattgactcg ccgcaaagtc ctctccggca gctttcgcag cgtattcagc
     5701 cgccttttcg cggttctcca gacgaaaacc cgtaaacaat ggcacctgcg ccgtcgcagt
     5761 caggttgtac gaattcagaa cggaaggccc gagagaaacc tgcgtcgagg gcgtcgtaat
     5821 gctggttccc ggaccggttg ggatcgtcag ggaaggcgtc atgatcaccg ggtccggcac
     5881 atcgctgagt cgcgcatacc gcgcgctgag cgacacaccc ggcaaccgcg ccgcgcgcgc
     5941 ctcgttcttc tgcgccgtgg ccgaaataac cgattgggca gaggctctga taccggcgtt
     6001 ctgggtcatc gcaagctcac gcgctcgcgc taccgtgagg cggagcgcgt tccccgcggc
     6061 ctccgtttga gcaacacccg aactccagag ccccagcccg gtgccgatag ccgcgaggct
     6121 tacgccgtac caccgtgtcc ttctcatgct gcggtgcctc cttcggaggg attaaccggc
     6181 ccttccgaat gccgtatctc ggatatgaat gcgtcctcaa gcgacggggg aatctcacgg
     6241 atggacgcca cgcggaattg catctcttcc agcgcctcct gaagccgttg cgtacccgca
     6301 accacgtcct ccaccggaac atggatgcgc gaaccgaaca tctgcgcttc ctcccattcc
     6361 ggtgcctgtt tcatggcgcg gtacgccgcc cgcgcatcct cacacacaac ttccagcagc
     6421 cgcttcccga atgtctccac aatcgccgcc ggggtgtccc ttcggataat cctgccctcg
     6481 tgcatcagcg ccaccgtgtg gcatcgctcc gcctcgtcca gatacggcgt cgccagcaca
     6541 acggtgatcc cctcgcgcag aaggtcggag aggatgatcc agaaatcccg gcgcgaaacc
     6601 ggatccaccc ctgtcgtcgg ctcatcgagg aatatcaggc cgggcgaatg gatcagcgtg
     6661 cacgccagcg ccagtttctg cttcattccg ccggaaaggt tccccgcaag acgccggcgg
     6721 aacggcgcca gccgcgtgaa cgagagaagc tcttccctgc gcttctggaa tcgccgtacc
     6781 ccgtgaatct gcgcgaaaaa ctcaatgttc tcgtcaaccg tcagatcgcc gtacagactg
     6841 aacacctgtg agagataccc gatactccgc tttaccgcgt cccgttcgcg aaccagatca
     6901 tgtcccagaa ccgtcgccgt cccggaggtc ggtttgagca caccggtcag catccggatc
     6961 gtcgttgtct taccggctcc gtccgggccc accagcgcaa gcatctcgcc ccggcccacg
     7021 gaaaaactca cgccgttcac cgcgtgcgtg gtgccgaaaa acctcgtcaa ttctcgggtt
     7081 tcaagcgctg ctgcgggcat ggttccgccg gcgcgcgcaa catggtccgt cattgcggtg
     7141 caccggctcc gtcaccggct cgaaacgccg cgtccgcggg cagtcccggt ttgaagaacc
     7201 cgtccggatt ctgaagcgaa atcttcacgg cgaacacgag cttcacccgg tcttccttcg
     7261 tctgcacgtt cttcggagtg aactccgcct tcggggatat ccaggatacc ctaccgctgc
     7321 gcggatgatc aggctcgccg tccagcgtta cctccgccgt gccgcccagc cgcacctttg
     7381 ccagctcgac ttccgtcaga tacaccttca gccagaccgg gtccagttcc gatagcgtca
     7441 caatggtccc gcccgaggga accatctccc ccggttccgc gatccgctgc gtcacgtaac
     7501 ccttcctggg cgacctcacc acgcagtcgg cgatccgttg agagacggca ctcaatgccg
     7561 cttccgcctg ctccacctgc gcccgcgccg ccgcgatctg ctccgaacgc gttcccgccc
     7621 gaagttttgc cagcgtcgcc cgtgccatcg cctgtgccgc ttcggcggac cgcacgcgcg
     7681 tccgcgcgtc atcaagctgt ttctggggtg ccgtcccggt cgcggcaagc tggctcaccc
     7741 gttccaggtc gagcctcgcc gcttccagcg caaccgaagc ctgttgttct gcggcctccg
     7801 cctgcgcgat gtcctcccgt tgcgggccgt tcaacgcaag ctggagattc gcccgcgcca
     7861 cacgcagttg cgcctcagcc tgcgatttct gccagtccag gtccgagtga tcaatcaccg
     7921 ccacaacctg cccagaatcc agcagatcgc cttcccgcgg cagaacctcc cgaaggatgc
     7981 ccggaacctt cgtggcgatg ttgatctcgg tgcactcaat aaccccgctc gccgtgatgt
     8041 ccccgtcaga tgcaccccgc gagcacgcgc ccaggccgat cgccagcacc ggagctaccc
     8101 aagccatttt tcccatttgt cttcccttgg aatcgtggac acccgtgtcg aatcctgttc
     8161 cttcagcacg ccgtgttgca ggatctccat gacgttccgc ctccggtcgg caaaaaattg
     8221 accggctttt cggtcttcta tctggagcat ggaactcaga agtggctctg cgaggaagga
     8281 aaacgcaacc atcccgagaa ccgagatcaa cgtgtgtttc gggtcaatct cgtggatgac
     8341 gccctcgtgg attccccgcc ggatcagatc aagcgccggt ttcgcgccga caaagctcgc
     8401 cctgtgctgc gcctgcggaa gcgcccgcat cacgatctcc ccgccggcca gcaggtccat
     8461 catgatcagc cgcggcacat ggggtttttt cgaaagggaa tcgaggtacg tgtcgaccag
     8521 catcaggaac cgcttctccg tgggcacgtc ttcgcccttc agaacgccct gcacgcgttc
     8581 catgatcccc tgaactgcag cggatacgac ggtttcgtac agcttttcct tgctggtgaa
     8641 gtagtaatgc agcatcgctt tgttgatttg tgcccggtca gcgatctcct gcattcgtgc
     8701 tccggatagc cctttgtcga cgaacacttc tgttgcggca tcgagtatcc gttggcgggc
     8761 atctgagtcc ctgttcatgg ggctttctcc tgtcgcagct caaccaatca gtcggtttaa
     8821 ccgaccggtt agttagcata cgaaatcact agccagcctg tcaagctcca gctcgaccgt
     8881 tccccgttac gacccccacc ggctggcccg tccggcgatc catcccgcca ccgcaatctg
     8941 cacggagaca agaaccgcac acacgccgat aaccgtcaaa aggctcagaa tcacgcgttc
     9001 agccagcatt tccgcgccaa aaagagaaag aacgacgcgc catgtatgcg gatcccccag
     9061 agcaccgaaa aagcccgcag caaggcactc cacgcccagt acgaagccga tgaaacgcac
     9121 ctgtttccga accccgcact ccccgccatg ccgctcgtgc gcggcgcgac gcccgacata
     9181 gaccgccgca aggcacccga ggagaaacgc gccaaaagga aaacccatga aaaacgcaaa
     9241 ggaaaacacc gaccagatga cgtacagaac cgcgagatgg cccatcggaa tcgagaacac
     9301 tctgcctgca tttctccggt accacaccca tgccagcaaa atccccgcca caaggcccga
     9361 acacgccgcc catgcgatcg ttgagtccgt gaggggaacc catctgagaa ttgacagaat
     9421 ggcggcgctc caccacgaca gaatgcccgc tgcaagggca attgacgcgc ctgtgagagc
     9481 tgcgaacaac ttcatctcca tccgcacgga accacccctt tgccgccaga gtccagacac
     9541 cgcttccgat gtgaataact gcgcagacgg gttcaggaca aaatcgccgc gccggcgggc
     9601 ggggcgggac gttgaccgct cgggaggttt tttatagcgt acgtaggcat acgcggcgtt
     9661 ttttctgcgt ctgttaccca ccttttccgc ccgcgaacga ttgccgcggg tgaatcccgt
     9721 aaccgagaga taccggatga acgttcctgt gcaggagaga atcgttgttc tcgattttgg
     9781 cgggcagtac gcgcatctta tcgccaaccg ggtgcgtcgg ctccgcgtgt tttcagaaat
     9841 tatgctcccg tccgagccgc cggcgaatct tgaaggtgtt cgcgggctga ttctttccgg
     9901 cgggccctcg tcggttcaca gcccggacca gcccccgttt gacgcccgct ggctccacac
     9961 cggattgccg gtgctcggcc tgtgctacgg ccaccaactg cttgcgaagg aactgggcgg
    10021 ggaagtgcgg cggggcgatc gccaggagta cgggattgcg gagcttcgca tcgctgacca
    10081 cgacgcgatt ttcgagggtc ttgagcccgt ggagcgcgtc tggatgagcc acggagactc
    10141 ggtaacccgc gttccggatg gatttgcagt tctgggtgga acggcggatt gcccaatcgc
    10201 cgcgatgggt gacgttcaga ggcgcatgtt cggattccag tttcatccag aagtgacgca
    10261 ctccccgcac gggcagaaaa tgctggacaa tttcctttct ctgtgcggat gcgcgcgcac
    10321 ctggacgatg gagcaattta tcacgcaggc cgaggagcgc atcaggaggc aggcgggtga
    10381 caggaacgtg ttcatgcttg tgtcgggcgg ggtggattcg tccgtggcgt ttgtgttgtt
    10441 gagcagggca ctgggggagc accgtgtcat cggtctccat attgacaacg gcttcatgcg
    10501 gctgaatgag tcggtgctgg ttaaggaatc gcttgaatgt gcggggttcc acaacctgca
    10561 cgtggtggac gcgagcgaga agttcctcgg ggcggtgaag ggggtgacgg atccccagca
    10621 gaagcggcag atcgtaggtg acacgttcat cagcgtgcaa caggaggagc ttgagcggat
    10681 cggcctcgac cccgatcact ggatgctcgg ccagggcacg ctgtacccgg acattataga
    10741 atcgggggga acccggcacg cgtcggtgat caagacgcac cacaaccgtg tggcgatcat
    10801 tcgtcagatg ctggaggaag gcaaaatcat cgagccgttg gaccagttgt acaaggatga
    10861 agtgcgacag gttggacggc agctcggtct gcccgacgcg ctggtggagc gccacccgtt
    10921 tccggggccg gggctttccg tccggtgtct ctgttcggac ggcgtggagg aaccgattga
    10981 ggcgtcggca aaggagaagg ttcgcgcact ggcggggagc ctcggatttg aaagcgaaat
    11041 cctgcccgtc aggagtgttg gcgttcaggg ggatgaacgg acgtaccggc accccgtggc
    11101 gctgtggcgc ccggacggcg gcgcggtgaa ctgggaggaa ctggaacgcc tttcaacgcg
    11161 catcacgaac accgtccccg aagtgaatcg cgtgatgttg ctggtgtatc cgcgttcgtt
    11221 gcctcggctg aagttgcgcg cacggtttct ggaccggccc cggctggatc tgctgcggct
    11281 ggccgatcac gccgcaatga gtgttctcgt tgaggatggg cttgcggggg attttttcca
    11341 gtttcccacc atccttattc ccctcggcga gcgcgaagaa ggcgaatcgg tggtgctgcg
    11401 cccgctcgaa tccacggacg tgatgacggc gagtttcgcg attcttccgg agcgttcgtt
    11461 gcgacggctg gtttcgcggc ttgcggcgct tcccgtggtg gacgcggtgt tttatgatat
    11521 cacccacaag cctccggcca ccatggagtg ggaataacgc gcggcagaag ggtccggcgg
    11581 agaccacccg gcaccccgat tgatgccggc cggggacacc gtcccgtcgg aagatcagaa
    11641 ggtgaggtgg cgtatgtacc acgcggcaaa ggcggagttc gcgctgacgg gagcgacggc
    11701 gcgcctgtac gagatctttc tggacgcgac gcgcggttcc gccgcggttg gtgacgtaaa
    11761 cagggcgctg ttcgagacag gcctcgtgca ccacgcgttg atgttgcttg cccttggcgt
    11821 ggtgccggaa gagcgtgcga agggagcccg cgctctcatt gatgagatcg gcaagaccac
    11881 gatcatgaaa gacagcttcg atcaggcccg cgaatactgg gagcgggttg cgaaggtgaa
    11941 tccttcggcg cccgagagca gtgatggatg aggctgtatc tgccgctctg cgagcgaacg
    12001 ggtacctgtg ggatctgtac acccgccggg aggaatacga gccgattttc ctcgacggac
    12061 tgggaagatt tcggtttttc gcgtcgcaac agcgggcgcc gtttgatccc gtcgtctcgc
    12121 gttttctttt tgaacgtggc gcatttcccg cttttcccgc cggcagacgc tacgcaatct
    12181 gcctctccca cgatattgac gcactccatt tgaatcggta ccgcagcggc ggagccggtc
    12241 tcctgagccg tgcaaggcgc gcaagtgcgc gttccgctga actgctcctt tcccggtcgc
    12301 tcaagcgagg tgatccggga tggaatctgg cgcgcatcgc cgacatcgaa aaagaacgcg
    12361 gcgcgaagag cgcgttctac tccctcgcgc tggaggaagg cgaaaaagac ttttacttcc
    12421 gggttcaaga ggttggcgcg cagcttcgca gtctcgtcgc gcgggggtgg gaagtggggc
    12481 tgcatggcgg gcacggggcg tattgcgatc cgtcggtact gagacgggaa aaaatccgcc
    12541 tcgaggtgga actcgggacg ccggtgacgg ggttccgcgc gcattatctg cgcttcgatg
    12601 ttcccgggac gtggcggtta ctggcggagg caggtttccg ttacgatacg acgttcgggt
    12661 atgcggactg tgtgggcttt cgaaacggga tgtgccaccc ctataccccg ttcgaccgta
    12721 acgctgatcg tttcattccg attctggaag ttcccctcgc ggtgatggat accacgcttc
    12781 aattgtacat gcggctcgac cccgcggacg cgtggaccgt tatccggcgt cttctggacg
    12841 cggtggcaga atgcagcggc gtgctgacgg ttctctggca tccgcagaac atgaacggcg
    12901 cctggggcga tctctacctg cgcatactcg attacggcac agacacagat gcatggctta
    12961 ccggacccgg cgaacttgcc ggatggtggc tttctcagca ggcggggggc aacgcctgag
    13021 gtatcgtgcc gcgtgacggc ataccgtacg cggtgcaaca ggaggactcg agatggttct
    13081 ggttgacaaa ggcgcgccgt gcagcgtggt agtgtcgggc gctcagccga cgtggctgga
    13141 acgccacgcc gcggaagagc ttgtccatta cctccgcctg atcagcggtg cggcccttcc
    13201 cacggtttcg ggtattccgg acggcatgaa tgcggtggta atcgggaggg cggacaccaa
    13261 ttcagttgtc gccgaggcgg tgcatgaggg agttctttct ctgtccgaag actcaccggg
    13321 ctgggacggt ttcgtgatca aaaccgcgcg gcgcggcaac cgcgatgtgt gttttgttgg
    13381 cggaagcagt gaccggggga cgctgtacgc ggtttactgg ctgctggaag aggtgctcgg
    13441 ggtgggcttc ttccgggatg gcgagtatat cccgaggagt gattctgtta tgctgccgga
    13501 atcgctgcac attgtggaac ggccggtgtt tgagcacaga gaggacggat tgctggcgta
    13561 ttcggccaag ttctggggat gggatgagtg gaagcgtgaa ctggactgga aagcgaagcg
    13621 ccgggcgaac atcacctggc cattttccgg caccggggag atcacgcagg cggtgctggc
    13681 tgaatggggt ttgaacgctg ccccgcctga gccgccggca gaacccaccc tcacggatcg
    13741 cctgcttgat tacgcgcaca aactggggat ccgtgtgccg tgtcacctcc cgagtggctc
    13801 catgcctgcg gtcttctggg aggcatttcc gaatgcgagg aagctggtga tgcaatggag
    13861 cgagtatgc
//
LOCUS       DAOSOO010000070        13817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4457_length_13817_cov_5.518093, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000070 DAOSOO010000000
VERSION     DAOSOO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4457_length_13817_cov_5.518093"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLT86_06755"
     CDS             <1..231
                     /locus_tag="PLT86_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36346.1"
                     /translation="TNTFIWKEKLIWYDLELDLWIPSCGSPQWQDEDDMRRLVRMEHL
                     SQEELDVAEQAKKYLDNHWRTVIECAFGEVHS"
     gene            324..1346
                     /locus_tag="PLT86_06760"
     CDS             324..1346
                     /locus_tag="PLT86_06760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT36347.1"
                     /translation="MALSDERFLRSRFDVRAFRIILAPPVRVLTNTATLAVWLCWNLP
                     RARGVFFRFADYYAFLPVFLSRLFRRKSWIVLGGYDAHHFPEYGYGVYEQPLRASIVR
                     YAVRNATAVLPVDQSLYDGVNAYAFDPPRPTGIKSLVPNFRSEVRVVADGFDHEFWTP
                     SPGCRKERLVVATASVPQGTSRESRMRMVVLKGVPLLLEVARRMPDVTFAIVGPDEGV
                     LPQGGQPVPPNVQLPGYLSPEELREYYRRAAVYAHPSLTEGLPAAVAEAMLCCCVPVG
                     SSVNGIPELIGDTGFVLTRPAVDEWVAAVTRALELGASSRARDRIVALFSLAKRHRML
                     LDVIEG"
     gene            1419..1781
                     /locus_tag="PLT86_06765"
     CDS             1419..1781
                     /locus_tag="PLT86_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT36348.1"
                     /translation="MPSIAIIGASKDRAKFGNKAVRAYVRQGCTVYPVNPKEREIEGI
                     LAYPSVKSIPFPVDRVALYVPPQIGIALLPEIAGKHPAELFVNPGAESQELLDEARRL
                     GLEPIVACAIVSVGEMPD"
     gene            1797..2252
                     /locus_tag="PLT86_06770"
     CDS             1797..2252
                     /locus_tag="PLT86_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT36349.1"
                     /translation="MRKVVKARPRLENSPSDGAKALFLEAVRALRPEDIPVKPVDRGE
                     GDASEPVARRRTERVVESIDLHGMTAEQAIRYLTEVLSDYRRTGGTVQIVVGKGLHSP
                     DGQGVLREVVSGWLDHAGKRFVSRWRWGGRRQGGNGVIVAAIRVPGKPD"
     gene            2268..3398
                     /locus_tag="PLT86_06775"
     CDS             2268..3398
                     /locus_tag="PLT86_06775"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36350.1"
                     /translation="MNRNLLRELIEPILNTPTGPFYEDHVASFVAETVRGTGLTMSTD
                     TFGNILVRRPSVGRNNLRIGFMAHMDHPAFEVIEQLEGAVRVAWHGGVDAEYFPQARV
                     RFHTPGNPCTGEIREVELEEARNRVKTAVVACDSPPSVGTLATWELPPVEVTNGVLRA
                     PAVDDVAGCGLLLALLLELGDRPLETEVWTLFTRAEEVGFVGAIGLARSGRLPVSTPV
                     VSVEMSKATAGVTQGMGPVIRLGDRSSIFDAMFVLFMKDVASDLKGRIEGFKYQQAMM
                     EGGSCEATALNLFGYRTAGLAVPLTNYHNQRPSGPSGPFVIDSEEIAINDFANAVDLC
                     VGMCERFRGLDAIRSGHRSKAVERAAEGLQRLERMERARTRR"
     gene            complement(3444..4517)
                     /locus_tag="PLT86_06780"
     CDS             complement(3444..4517)
                     /locus_tag="PLT86_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT36351.1"
                     /translation="MTYGVIMAGGKGTRLWPEGRKTRPKQLLDLIDHRPMLRVAVDRL
                     APLIPVERQIIVTTREYASEIARALPAFPAENILVEPLGKDTAPCIGWAALKVTERDP
                     TATMAVVTADHFIADETGFREVLHAAVTVAENASLVVVVGFVPLRPDTGLGYIRFGHE
                     TREIEGIQFYRVDEFVEKPNKAKAIQYVEDGRYLWNSGMFVMQARHALNLFSRFLPRH
                     FALLEEIKSSLGTPDESAVTENAYQRFDRVSLDVGIMEKADNVEVIRADFGWLDIGTW
                     AALDQISLHDARGNVVRGQHVGLDTERCIIRTSNSVVATMGVHDLVIIETDAAVLVCP
                     KSRVQEVKNLVEALEREGREDVT"
     gene            complement(4534..6009)
                     /locus_tag="PLT86_06785"
     CDS             complement(4534..6009)
                     /locus_tag="PLT86_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT36352.1"
                     /translation="MVRIHHLVAALAFTVLMSSCLPVRQTEPTPRPVALHPDDLPPFD
                     SLKFTPPPVFRTTLPNGITLFVLTDNEQPIVNVDILIRGGSQCDPAGKPGVASFTAGV
                     MRRGGTLHLSADALDDTLDYLAAHLNVGMDVDCASANLNVLRKDFDSGLAMLADVLIS
                     PAFPENKIVELQRLTKESIRRRNDDPYEIGRRRFRQIVYGQGNPWARQLELEDVDRIT
                     REDLVAFHRTWFRPNVTYMAISGDITPEEAVAKVSRAFAGWAVAEVPPLPSPEQNHGM
                     PPGVYHIQKAVNQSAIRMGSTGLRRHDPDQYAVAVMNQIFGAGTFTSRLGQEVRSNRG
                     LAYYVFGIVLDSPDPDQGMFLAAVGTRADKTSETISVMRSVIDSMSIKPVTDDEISAA
                     KETIINSFVFSYASPERIAGQEMRLMFDGYPEDYLTSYIPRIRSVTKDDVVRVARKYL
                     RSDQLRVLVVGDSTRFDGDLGALGPLTSLPPDDPSGDQAPR"
     gene            complement(6012..7685)
                     /locus_tag="PLT86_06790"
     CDS             complement(6012..7685)
                     /locus_tag="PLT86_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT36353.1"
                     /translation="MVRIVFLLVCALCVLPAAPSYAQPLEERVVEHTLDNGMKFLFVK
                     RGTAPVFSGVVIVRVGGVDDPQGQSGLAHLFEHMAFKGTPRIGTVDFAAEQGIMRRID
                     SVAVLYTQTLATVPLAHRGALEALERRTAREVVVANQGSEDYEITRALANALAETLRT
                     APDRYPSLRAYARAKGYLADLQRYTAIHRTFIVKDEFSRIIDRNGGVDNNAGTGKDYT
                     IYYESFPANRLELWALLESERFMNPVMREFYSERDVVAEERRMRTDDDPEGKLYEQFM
                     ATAMQAHPYRIPIVGWMSEVEKVTAQDAAAFREAYYVPSNTVGVIVGNISVDSAKAFV
                     DRYFGRIPSTNRRAPEVRTVEPPQAGERRVSVDFDAEPQLMVGYHKPNYPDPDAYVFS
                     VIANILRDSGNSSRLYRRLVQTGIASDVAVYEELPGQRYPNLFVLHASPAAPHTTGEV
                     EEILYSELDSLARVPVSEAELTKCKNQLEASYIREISDNNSLAQALARAQAIHGDWRV
                     VTEHRANVSKVTADDVMRVARLYFKPQNRTVAAIVRENEQNNGRGREDQ"
     gene            complement(7700..8824)
                     /gene="rlmN"
                     /locus_tag="PLT86_06795"
     CDS             complement(7700..8824)
                     /gene="rlmN"
                     /locus_tag="PLT86_06795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT36354.1"
                     /translation="MVQPAVCAERPDLKGFLPDELRDFLARFGKEKYRTNQLLRWMHG
                     KGVADFDQMTDLGKEFRSQLKAEARVECLAERHRAVSRDGQTTKLVLDLSDGRKIETV
                     MMVDDGRRTVCVSSQAGCALGCRFCATGQIGFQRNLTVGEILDQIHHAARFFRARGAT
                     GEEYEHPVTNVVFMGMGEPLNNYEVVLRATKLSGLEMGLAISATRVTISTAGVVPMIY
                     RLASDLPKVGLAISLNATTDEVRNSLMPINRKYPLKTLLDAARHMAGVSPRRRVTFEY
                     ALIEGTNDSDEDARRLAELVSGFPCKINLIPFNPVPRQPFRRPSQERIDRFHRILWER
                     NLTVTVRWSKGDDIAAACGQLQASVPYRSLHGNPSGTETP"
     gene            9003..9767
                     /locus_tag="PLT86_06800"
     CDS             9003..9767
                     /locus_tag="PLT86_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36355.1"
                     /translation="MVYRFLLAVVATSVICVFVQACTPSRSLPQISTREVPDPFVRST
                     QDASDDSLVRAKWMCRTTVPDPLAGQEDGPKIRFEATLYGEDVIVAEVVKECRGPEGL
                     VASDTCVSASRERYRSVHRDDTRLRIRLDLESSFSVNSLNSRFWDVYLKDERGIAYEP
                     VEIVQNAPVIVREDTLPEPGRAPVRGAVYRRAIDMYFPREDAFGTDVISSSTREVRLV
                     VSQNRRELTTFVWRIKRTGDSGTARTGSAQRAPSGF"
     gene            9791..10186
                     /locus_tag="PLT86_06805"
     CDS             9791..10186
                     /locus_tag="PLT86_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36356.1"
                     /translation="MNTHEELVEKVETLAERDGRFRKEAYFFVFEALQFTVKSLNLTG
                     KQRHVTGQQLAHGIKEYALDRFGPMTLSVFEHWGVRSTLDFGEIVFGLVEAGLMGKTE
                     EDSIEDFVDVYDFQKAFDWHNAIGRKLRK"
     gene            10183..10992
                     /locus_tag="PLT86_06810"
     CDS             10183..10992
                     /locus_tag="PLT86_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT36357.1"
                     /translation="MTSEQPQHTGKPFVLASCSILPEKWNKEGNAEKMLSFMERSLRC
                     GADVIAVPEGCLEGYVVMEAIEGNRGPELLNIAEPEDGEMIGRFRSFCQKHHVHALIC
                     FCERERNEAFNTALWIGRTGETVGKYRKTHLNEGYNPDHFFNRTGDEIRAFDTEFGRA
                     GLMICFDRRVPEVARCLALDGAQFILNPSYGVYKGWNDSVLVARAHENQVPVVFVHPN
                     KTLAVNEKGDITFCREETDVISHLTITPRTSSSLLLSLRRPELYGRLTSLR"
     gene            11138..13063
                     /locus_tag="PLT86_06815"
     CDS             11138..13063
                     /locus_tag="PLT86_06815"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOT36358.1"
                     /translation="MAGKSICHKIIEQHLVAGKVIPGTEIGIRIDQTLTQDATGTMAY
                     LQFEAMGIPRTRAGLSVSYVDHNMLQDGFENADDHRYLQSVARKFGVYFSRPGNGICH
                     QVHRERFGVPGQTLLGSDSHTPTGGGIGMLAMGAGGLDVAVAMAGHPYFFPCPRVTLV
                     RLSGRLRPWVGGKDIILELLRRLSVKGGVGRVMEYGGPGVATLPVADRFTVANMGAEL
                     GATTSVFPSDELTRKYLEAQGRPEAWIPMYADPDATYEAVVEIDLDKLEPMIARPHSP
                     DNVAPVRDLEGTPVQQVCLGSCTNSSYHDLRIAAAMLRGRTISPAVSLTVTPGSRQVL
                     EMIARDGSLADLIAAGARVLEAACGPCIGNGQAPPSGANSVRSFNRNFEGRSGTRDAG
                     VFLASPETCVACAITGVITDPRDLGIEFPEIPEPESYSRNDSMVIPPSTEPEEVTVSR
                     GPNIVSLGSFEAMADTLRGRVLIKVGDNITTDHIMPAGRWLKYRSNVPRYSEATFNLI
                     DPDFPKRSREWGGGLIVAGENYGQGSSREHAALVPRWMGVKAVIAKSFARIHEANLVN
                     FGILPLVFADPDEYGRIQQGDTIEIPHARQAVENGLNVIEATNLTQGAKFGLRVNLTE
                     RGRKVVLAGGMLNFVRS"
     gene            13073..>13817
                     /locus_tag="PLT86_06820"
     CDS             13073..>13817
                     /locus_tag="PLT86_06820"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOT36359.1"
                     /translation="MENSPSENQLLVGAHMSTEGGLFRAVERGAQIGCTAIQLFTRNP
                     SQWFSPPLSEKDVTEFRDAVSRTKMAAVVAHDSYLINLASPDASLRKRSIAAMLDELD
                     RASALGIPLLVTHLGAHTGQGEENGLQTLVDSLDEVTDARPDAPVSVCLETTAGQGTS
                     LGWAFEHIAWVLSKLSAPGRFRTCFDTCHVFAAGYDLVTRDTYEKTWREFDKVVGIRY
                     LAVMHLNDAKKELGSRVDRHAHIGKGEIGE"
BASE COUNT         2881 a         3777 c         4142 g         3017 t
ORIGIN      
        1 acgaacacgt tcatctggaa ggaaaaactg atttggtacg accttgaact cgatctctgg
       61 attccttcgt gcggatcgcc gcagtggcag gacgaggatg acatgagacg acttgtcagg
      121 atggagcatc tcagtcagga ggaactggat gttgccgaac aggcgaagaa gtatcttgac
      181 aaccactgga gaaccgtgat tgagtgtgcg ttcggggagg tgcactctta gaaaacaggg
      241 caacaagttt gcgccgggga ggcggtttct tggcacgaag ctcgaacgat cgcattctca
      301 tccttcagac atcaagagcc ccggtggcac tctcggatga acgcttcctc cgttcacggt
      361 tcgatgtacg agcttttcgt atcattcttg cgccgcccgt tcgtgtgctg accaacacgg
      421 caaccctagc tgtatggttg tgctggaacc ttccgcgagc acgtggggtt ttcttccgct
      481 tcgcggatta ctacgcgttc ctgcccgtgt ttctgtcgcg gctgttcaga cgcaagtcat
      541 ggatcgtcct cggcggatac gacgcccacc atttcccgga atatggctac ggcgtctacg
      601 agcaaccact gcgagcatca attgttcggt acgccgttcg gaatgcaacc gcagtgttgc
      661 ccgtggacca gtcgttgtac gatggcgtga acgcgtacgc gttcgatccg ccccgtccca
      721 cggggatcaa atcgctggtt cccaatttca ggagcgaagt ccgggttgtt gcggacgggt
      781 ttgatcacga attctggacg ccctctcccg gatgtcggaa agaacggctg gtggtggcga
      841 ccgcgagtgt tccgcagggt acgtcgcggg agagccgcat gcggatggtg gtgctcaaag
      901 gtgttccgct tttattggag gttgcccggc ggatgccgga tgtcaccttc gcgattgttg
      961 gtcccgacga gggagttctt cctcaggggg gccagcccgt tccgccgaat gtccagttgc
     1021 cggggtatct atcgccggag gaactccgcg agtattaccg gagagcggcc gtatatgcac
     1081 atccgtctct taccgagggg ttgcctgctg ccgttgcgga ggcgatgctt tgttgttgcg
     1141 taccggtcgg ttcctcggtg aacggtattc cggaactgat cggggatacg gggttcgtac
     1201 tgacgcggcc ggcggttgac gaatgggtcg ccgcggtgac tcgtgcattg gaactcggtg
     1261 cgtcttcacg ggcgcgagat cgaatcgttg ctcttttcag cctggcgaag cggcatcgca
     1321 tgctgctcga tgtgattgag ggatgagtct ggtggctatc cgtcggggaa gaagggtcga
     1381 atcagaaaac cgttggaagg tgtcatagga gaaggcagat gccatcaatc gcgattatag
     1441 gcgcatcgaa agaccgtgcg aagttcggta acaaagccgt tcgggcttac gttcgtcaag
     1501 ggtgcaccgt gtacccggtc aatcctaagg aacgcgaaat agaagggatc ctggcgtatc
     1561 cttccgtgaa gtcgattcct tttccggtgg acagggttgc cctttacgtg cctccccaga
     1621 tcggcatcgc actgctaccc gagatagccg gaaagcatcc cgcggagttg ttcgtcaacc
     1681 ctggtgcgga aagtcaggag ttgctggacg aagccagaag gttgggtctg gaaccgatcg
     1741 ttgcctgtgc gatagtgtca gttggggaaa tgccggatta ggatggacgg acagcgatga
     1801 gaaaggtcgt gaaggcacgg cctcggcttg agaactcgcc atcggacgga gcgaaagcgc
     1861 tctttctgga ggcggtgcgc gcgctccggc cggaggacat cccggtcaaa ccggttgatc
     1921 gtggggaagg cgacgcctct gaaccagttg ccagacgtcg tactgagagg gttgtggagt
     1981 ctatcgatct tcacggcatg actgcagaac aggcgatcag gtatctcacg gaagtgcttt
     2041 cagattaccg acgaacgggc ggaacggtcc agatcgttgt cggcaaaggg ctgcacagcc
     2101 ccgacgggca gggagttttg cgcgaggtcg tctctggctg gctcgaccat gcaggaaaac
     2161 gtttcgtctc gcgatggcgg tggggtgggc ggagacaagg gggaaatggt gtcatcgtcg
     2221 cggcaatccg cgttcctgga aaaccagact gaaagcaggg tggtgtcttg aaccggaatc
     2281 ttcttcggga actcatcgag ccgatcctca acactcctac ggggccattc tacgaggatc
     2341 acgtggcgtc cttcgtcgcg gaaacagtac gcggcacagg actcacgatg agcactgaca
     2401 cgttcgggaa catccttgtc cgccgcccct cagtcggcag gaacaacctc cgtatcgggt
     2461 ttatggcgca catggatcac ccggcctttg aagtgataga gcagctcgag ggagcagttc
     2521 gtgttgcgtg gcacggaggt gtggatgcgg agtacttccc gcaggcgcgc gtcagatttc
     2581 acacccccgg caatccgtgc acgggtgaga ttcgagaggt ggagcttgaa gaagctcgta
     2641 acagggtgaa aaccgccgtt gtggcgtgcg actcacctcc atccgttggg actctcgcaa
     2701 cgtgggaact gccaccggtg gaggtaacga acggggttct gcgtgcgccg gcggtggatg
     2761 acgtcgcggg atgtggcctt ctgttggcgc tactcctcga actgggcgac cgaccgctgg
     2821 aaacggaggt ttggaccctt ttcacccgag cagaagaagt cgggtttgtg ggtgcgatcg
     2881 gactggcgcg aagcgggcga ttaccagttt cgacgcccgt tgtttctgtg gagatgagca
     2941 aagcgacggc gggcgtgacg caggggatgg gtccggtaat ccgtctcgga gatcgatcaa
     3001 gcatttttga cgcaatgttc gtgctcttca tgaaagacgt ggccagcgac ctcaaagggc
     3061 gtatcgaggg cttcaaatac cagcaagcga tgatggaagg gggcagttgc gaggcaactg
     3121 cgctcaatct ttttgggtac cgaaccgccg gtctggcagt tccactgaca aactaccaca
     3181 accagcgccc ttccggcccg agtggcccct tcgtcataga ttcagaggaa attgccatca
     3241 acgactttgc aaatgccgtg gatctatgcg ttggaatgtg tgaacgattt cgcggccttg
     3301 acgcgatcag gagcgggcac agaagcaaag ccgtggaacg cgcagcggag ggtttgcagc
     3361 gtctggaacg aatggagcgc gcaagaacac gcagatgagt gctcagttgc tcgatgaacg
     3421 gttcgagcgt tgcgttaccg acttcaggtc acgtcctccc gtccttctct ctccagcgct
     3481 tctaccaggt tcttcacctc ctgtacccgc gacttcgggc acaccagcac cgcagcgtcg
     3541 gtttcaatga tcacaagatc gtgcactccc atcgtcgcaa cgacggagtt gctggttcgg
     3601 ataatacagc gttcggtatc gagacccacg tgttggcctc gtaccacatt gccccgggcg
     3661 tcgtgaaggc ttatctgatc gagagcagcc cacgttccaa tatccagcca tccgaaatca
     3721 gcccggatca cctcgacgtt atctgccttt tccatgatgc ccacgtccag cgagacccgg
     3781 tcgaaacgtt ggtacgcgtt ctcagttacg gcagattcgt cgggcgttcc aagagacgat
     3841 ttgatctctt cgagcagggc gaagtggcgg gggaggaagc gcgagaaaag gttcagtgca
     3901 tgccgtgcct gcatcacgaa catcccggag ttccacagat atcgcccgtc ttcgacgtac
     3961 tgaatcgctt tggccttgtt cggtttttcc acgaactcgt cgaccctgta gaactggatt
     4021 ccctcgattt cacgtgtttc gtggccaaaa cgaatgtacc cgagaccggt atccggtctg
     4081 agcggcacga accccaccac caccacaaga gaagcgtttt ccgcgacggt aaccgcggcg
     4141 tgcaggactt cgcggaaccc cgtttcgtcg gcgatgaaat gatccgcggt gactactgcc
     4201 atcgtcgcgg tcgggtcgcg ctcggttacc ttcagagccg cccatcctat gcacggggcg
     4261 gtatctttgc ccaagggttc taccaggatg ttttccgcgg gaaacgccgg cagagcacga
     4321 gctatttccg atgcgtactc gcgcgtcgtt acgatgatct gccgctcaac tggtatgagc
     4381 ggggcaagcc gatcaacggc cacgcgaagc atcggccggt ggtctataag gtcaagcaat
     4441 tgcttgggtc gggttttcct tccttcaggc caaagccgcg tgccttttcc acccgccatg
     4501 attacgccgt acgtcatgcg tcccccgggg tatttaacgt ggcgcttggt cgccggaagg
     4561 atcatctggc ggcagagatg tcaaaggacc gagggccccg aggtccccat cgaaccgggt
     4621 agagtcgccg acaacgagaa cgcgcagttg gtcgcttctc aggtatttcc gcgccactcg
     4681 taccacgtca tcctttgtta cggaacggat ccgagggatg taagacgtga gataatcctc
     4741 ggggtacccg tcgaacataa ggcgcatctc ctgtccggcg attcgttcgg gcgaggcata
     4801 agagaacaca aacgagttga tgatagtctc tttcgcggcg ctgatttcgt cgtcggtaac
     4861 aggtttgata ctcatgctgt caatcacgga ccgcattacg gagatcgttt ctgatgtctt
     4921 gtcagcacgg gtgccgaccg ctgcaagaaa catcccctga tcgggatcag ggctatccag
     4981 cacgattccg aatacgtagt acgccaggcc gcggttcgaa cgaacctcct ggccgaggcg
     5041 ggacgtgaag gttcccgctc cgaatatctg gttcatcacg gcgacggcgt actggtctgg
     5101 atcgtgtctt cgcaatcctg tgcttcccat ccggattgcg gactgattca ctgctttctg
     5161 gatgtgatag acccccggag gcattccgtg gttctgctca ggtgatggca gcggcggaac
     5221 ctccgcaaca gcccacccgg cgaaagcccg actcactttg gcgacggcct cttctggtgt
     5281 aatgtctcca gaaatcgcca tgtaggtcac gttcggtctg aaccacgtcc ggtggaacgc
     5341 gacgagatct tctctggtga tcctgtcgac atcctccaat tcgagctgtc gtgcccaagg
     5401 gttgccttgt ccatacacga tctggcggaa acggcgcctt ccgatctcgt aggggtcatc
     5461 attccgccga cgaatgcttt ccttcgtcaa tcgttggagt tcaacgatct tgttctcagg
     5521 gaatgccggc gaaatcagca catcggcaag catggcgagg ccggagtcaa aatccttgcg
     5581 cagcacattc agattggcac tggcgcagtc cacgtccatt cccacattca ggtgtgctgc
     5641 gaggtaatcc agcgtgtcgt ccagcgcgtc ggcgctcaaa tgcagcgttc caccgcgtct
     5701 catcacgcct gcggtgaacg aggcgacccc cggcttccct gcggggtcac actgggatcc
     5761 gcctctgatc aggatgtcga cattcacaat tggctgctcg ttgtcggtga gaacgaacag
     5821 cgtaatcccg ttcggcagcg tggttcggaa aaccggcggc ggcgtgaact tcagcgagtc
     5881 gaacggaggg aggtcgtcgg ggtgtaacgc aacgggacga ggggtcggct cggtttggcg
     5941 gaccggcagg cacgaactca tcaaaacggt gaaggccagt gccgcgacca ggtgatggat
     6001 tcgcaccatg gttactggtc ctcccttccg cgtccattgt tttgctcatt ctcgcgaacg
     6061 atggctgcta ccgtccgatt ctggggtttg aaatacaacc tcgccacgcg catcacgtca
     6121 tccgccgtta ccttggagac attcgctcga tgctcagtaa ccacccgcca atctccgtgg
     6181 attgcctgcg cacgcgcaag cgcctgagcc agtgaattgt tgtccgagat ttcgcggatg
     6241 tacgacgcct caagctggtt tttgcatttt gtgagctctg cctctgagac aggcaccctc
     6301 gccaggctgt cgagttccga gtaaagaatc tcttccactt cccccgtcgt atggggcgct
     6361 gccggcgagg cgtgcagtac aaacagattt gggtacctct ggccgggcag ttcttcgtac
     6421 acggccacat cgctggcgat accggtctgg acgagtctgc ggtacagcct ggaggagttc
     6481 ccgctatcgc ggaggatgtt ggcgattacg gaaaaaacat atgcatccgg gtccgggtaa
     6541 ttcggcttgt ggtatcccac catcagctgc ggttccgcat cgaagtccac cgagaccctt
     6601 cgctctccgg cttgaggtgg ttctacagtc cgcacctcag gcgcccggcg atttgtcgaa
     6661 ggtattcgtc caaaatacct gtctacgaaa gcctttgcac tgtcaactga gatgttccca
     6721 acgattacgc cgacggtgtt gctggggacg tagtacgcct cccggaacgc cgccgcgtcc
     6781 tgggcggtaa ccttctcgac ctcgctcatc caccctacga tcgggatacg ataaggatgg
     6841 gcctgcatcg cggttgccat gaactgctcg taaagcttgc cttccgggtc gtcgtccgtt
     6901 cgcatgcggc gctcttctgc tacgacgtcc cgctcgctgt aaaactcccg catcacgggg
     6961 ttcatgaaac gttctgattc cagcaacgcc cacaactcaa gtctgttggc gggaaaggac
     7021 tcgtagtaga tcgtgtagtc ctttcccgtt cccgcgttgt tgtcgacccc accgttcctg
     7081 tcgattattc gcgagaactc gtcctttacg atgaaggttc tatggatcgc cgtgtagcgc
     7141 tggaggtccg ccaaatagcc ttttgcccgg gcgtacgcac gaagagacgg gtaccggtcg
     7201 ggtgccgtgc gcagggtttc cgcgagggca ttggcgagcg ctcgggtgat ttcgtaatcc
     7261 tccgaaccct ggttggcaac aaccacttcg cgggcagttc tccgttccag cgcttcaaga
     7321 gccccgcggt gcgcaagcgg gacagttgcg agtgtctggg tgtacagcac ggcgacactg
     7381 tcgatacggc gcatgattcc ctgttcggcg gcgaaatcca ccgtgccgat gcgcggcgtg
     7441 cccttgaacg ccatgtgttc gaaaaggtgc gcaaggccgg attggccctg aggatcgtct
     7501 actcctccaa cgcggacaat cactactccg gagaacactg gcgcggtgcc tctcttgacg
     7561 aaaagaaact tcatcccgtt gtctagggtg tgctcaacga cccgctcctc gagtggctgc
     7621 gcatagcttg gggctgccgg caacacacac aacgcgcaaa ctaggagaaa aacgattcgt
     7681 accaacagag gactctcctt catggtgttt ctgtaccgga gggattaccg tgtagtgatc
     7741 gatatggcac cgaggcctga agttgaccgc acgccgcagc gatgtcgtcg cccttgctcc
     7801 aacgtacggt gaccgtcaga ttcctctccc agagaatacg atgaaaacgg tcaattcgtt
     7861 cctggctggg acggcggaat ggctggcgtg gtacaggatt gaaaggaatc agattgatct
     7921 tgcaggggaa tccggatacc agttcggcca gtcggcgcgc gtcctcgtcc gagtcatttg
     7981 ttccctcgat aagtgcgtac tcgaaggtca cccgccttcg gggagaaact cccgccatgt
     8041 gccgcgcggc gtccagaagt gttttcaatg gatatttcct gttgatcggc atcaacgaat
     8101 tgcggacttc gtctgtcgtc gcgttaagcg agatcgcgag acccactttg ggaagatcac
     8161 tggccaatcg gtatatcatc ggcactacgc cggctgtgct gatcgtcact ctggtggcgg
     8221 agatcgccaa ccccatctcg aggccggaca gttttgtggc tcgcagaaca acctcgtagt
     8281 tgttcaaagg ttctcccatt cccatgaaaa caacgttcgt aacgggatgc tcatattcct
     8341 ctccggttgc tccccgggct cgaaagaaac gggcggcatg gtgaatctgg tcgagaatct
     8401 ctcccacggt cagattccgc tggaagccaa tctggccggt ggcgcagaaa cggcagccga
     8461 gcgcacatcc cgcctgcgat gaaacgcaga ccgtgcgacg accatcgtcg accatcatca
     8521 ctgtttcgat tttgcggccg tctgaaagat caagcacgag cttggttgtt tgtccgtcgc
     8581 gtgagactgc tcgatgccgc tcggcaaggc actccacccg cgcctccgct ttgagctgcg
     8641 acctgaattc cttcccaagg tccgtcatct ggtcgaaatc agcgacacct tttccgtgca
     8701 tccagcgaag gagttgattg gtccggtatt tttcctttcc aaaacgtgcc aggaaatcgc
     8761 gcaattcatc gggcaggaaa cctttcaggt cgggtctttc tgcacaaaca gcgggctgta
     8821 ccaaggctga ggcaccatcg aaggattctt cgggaatggt tggaagcata ttggaaaaat
     8881 accctcgcgg atgtcattcc gccagcgctc cgttcaccgg tcgatgcgct gcttgaccgt
     8941 tcttcgccaa taagggtata cttcaaaact accgaaatgc gcgttttacg ccggaggtaa
     9001 ccgtggtgta ccgatttctg ttggctgtcg ttgcgacgtc tgtgatttgc gtgtttgttc
     9061 aggcctgcac gccgtcgcgt tctttgccgc agatttcgac tcgagaagtt cccgatccgt
     9121 tcgtcaggag cactcaagat gcctcggacg acagcctcgt tcgagcaaaa tggatgtgcc
     9181 gtacaacggt tcccgaccct ttggcagggc aggaagacgg gcccaagatc cggtttgaag
     9241 caacgctgta cggtgaagat gtcatagtag cggaggttgt caaagagtgc cgcgggccgg
     9301 agggtctggt cgcctctgac acgtgtgtgt cggcttctcg cgaacgttac cggagcgttc
     9361 accgcgacga cacacggctc agaatccgtc tggacctgga atcaagtttc agcgtgaatt
     9421 ccctgaattc acggttttgg gatgtgtatc tgaaagacga gcgaggcatt gcgtacgaac
     9481 ccgtcgaaat tgttcagaat gccccggtca ttgtccgcga ggacaccctc ccggaacccg
     9541 gtcgtgcgcc ggtgagaggc gcggtgtacc gccgcgcgat cgacatgtac tttcctcggg
     9601 aagacgcgtt tggcacagat gtcatttcca gttcaacgcg tgaagtacga cttgtcgtgt
     9661 ctcagaaccg gcgtgaattg accacgtttg tctggcgcat aaaaagaact ggcgactccg
     9721 gaaccgcaag gactggcagt gcacagcgtg cgccatcggg tttctgatca caccggattg
     9781 gttcagaccc atgaacaccc acgaagaact ggtcgaaaag gttgagacac ttgccgaaag
     9841 ggatggacgt ttccgtaagg aagcctactt ctttgtgttt gaagcgctcc agttcacagt
     9901 caaatccctc aacctgacag gcaaacagcg acacgtgaca gggcagcaac tcgcccatgg
     9961 gatcaaggaa tacgctcttg accggtttgg cccgatgacg ttgagcgtgt tcgagcactg
    10021 gggcgttcgg tctacgttgg atttcggcga aattgtgttc ggcctggttg aggccggtct
    10081 catggggaaa accgaggagg attccatcga agatttcgtg gacgtgtacg acttccagaa
    10141 agccttcgac tggcacaacg caattggcag aaagctgcgc aaatgacatc cgaacaaccg
    10201 cagcacacag gcaagccttt cgtccttgcg agttgcagta ttcttcccga gaaatggaac
    10261 aaagagggaa acgccgagaa gatgctctcc ttcatggagc gatcgctgcg gtgcggtgct
    10321 gacgtcatcg cggtgcccga aggctgcctt gaaggctacg tggtaatgga agcaatcgaa
    10381 gggaaccggg gaccggaact gttgaacatc gccgaaccgg aagatggtga aatgatcggg
    10441 cgtttccgga gtttctgcca gaagcaccat gtgcacgcgc tgatatgttt ctgtgagagg
    10501 gaaaggaacg aggctttcaa caccgccctg tggattggcc ggaccggcga aacagtgggc
    10561 aaatacagga agacacatct caatgaagga tataacccgg atcacttttt caacagaact
    10621 ggggacgaaa ttcgtgcatt cgacacagag ttcgggcgcg caggactgat gatctgtttt
    10681 gaccgacgag ttcccgaggt tgccaggtgc ctcgccctcg atggtgcgca attcattctg
    10741 aacccttctt acggcgtgta caagggatgg aacgactccg ttctggttgc gcgtgcgcac
    10801 gaaaatcaag ttccagtggt atttgttcat ccgaacaaaa cgttggcggt taacgaaaaa
    10861 ggcgatatca cattctgccg cgaagaaacg gatgtgattt ctcatcttac gattacgcca
    10921 cggacctcgt cctcgcttct cctttcgctc cgtcgccccg aactgtacgg gcggctcacc
    10981 tccctcagat agttcacagg ggccaggtgc cgaagttccc aacggtggcc cgcagcaaac
    11041 gaaatcgtta taatctctta tgccgtctgc gccgcaggtg tggcgctttc tgacgggggt
    11101 gcgcaacgag caaaaaacag tcgggaggta acattcaatg gcgggcaaaa gcatctgcca
    11161 caagatcatt gagcagcacc ttgtcgcggg taaagtaatt cctggaactg aaattggaat
    11221 ccgcatagat cagacactca cccaagacgc aaccggaacg atggcctacc tccagttcga
    11281 agcgatggga atacccagga caagagccgg attgtcggta agctacgttg atcacaacat
    11341 gctgcaggat ggctttgaaa acgctgacga ccatcggtac ttgcagtccg tggcccgcaa
    11401 attcggcgtg tattttagtc ggccggggaa cggaatctgt catcaggtgc acagagaacg
    11461 gtttggtgtt cccggacaaa cgcttttggg aagcgacagc cacacaccga ctggcggtgg
    11521 catcggcatg ctggcgatgg gtgccggtgg attggacgtc gcggttgcca tggcagggca
    11581 cccgtatttc ttcccgtgtc cccgcgtgac gctcgttcgg ctctcaggcc ggttgcgtcc
    11641 gtgggtagga ggaaaggata tcatcctcga actccttcgc cgcctttcgg tgaaaggtgg
    11701 cgtgggccgc gtaatggaat acggcggtcc gggtgtggcc acgcttccgg tggcggatcg
    11761 ttttaccgtc gcgaacatgg gcgcggaact cggtgcgacc acgtctgtgt ttcccagcga
    11821 tgagctgacg cggaagtatc ttgaagctca aggccggcca gaggcgtgga ttcccatgta
    11881 tgcagacccg gacgcaacgt acgaggctgt ggtggaaata gatctggata agttggaacc
    11941 catgatcgcc cggccacata gccctgataa tgttgcacca gtgcgtgatc ttgagggtac
    12001 gcccgttcag caggtctgcc tcggcagttg caccaactcc tcctaccatg atctccgaat
    12061 agctgccgcg atgctccgtg ggaggacaat aagcccagcc gtctcattga cggttacccc
    12121 cggttcaagg caggtcctgg aaatgatagc tcgtgacggc tcgctcgcgg atctgattgc
    12181 tgccggagcc cgcgttctgg aggccgcctg cgggccgtgc atcggaaacg ggcaggctcc
    12241 tccctctgga gcgaacagcg tgcggagctt caacaggaat ttcgaagggc gttcgggaac
    12301 gcgcgacgcg ggggtgttcc tggcaagccc ggaaacgtgc gttgcgtgcg ccatcacggg
    12361 cgtcattacg gatccgcggg atctcgggat cgaattccct gagattcccg aaccagaaag
    12421 ctattcccgg aatgacagta tggtcatacc gccttcgacc gaaccggaag aagtcactgt
    12481 gtcacgaggt ccgaacatcg tatcgctcgg ttcgttcgaa gcgatggcag atacccttcg
    12541 agggcgcgtt ctgatcaaag tcggagacaa cataacgaca gaccacataa tgccggcggg
    12601 aagatggctg aagtaccgtt ccaacgtccc cagatattcg gaagcgacct tcaacctgat
    12661 agatccggat ttcccgaaac gttccaggga atggggtgga gggctcatcg ttgcgggtga
    12721 gaactatggt caggggtcga gtcgcgaaca cgcggcgctg gtgcctcgct ggatgggtgt
    12781 gaaagcggtg attgcgaagt cgtttgcgcg gatccacgag gccaatctgg tcaatttcgg
    12841 tattctccca ctggtattcg cggatccgga tgagtacgga cggattcagc agggcgacac
    12901 gatagagatt ccgcacgcgc gccaggcggt cgaaaacggg ttgaatgtca tcgaagcgac
    12961 gaatctcacg cagggtgcca aattcggtct gagggttaat ctcacggagc gaggtcggaa
    13021 agtggttctt gccggcggca tgctgaactt cgttcggagt taggagctat ccttggagaa
    13081 ctcaccgagt gagaatcagt tgctcgtcgg tgcccatatg tccaccgagg gagggttatt
    13141 ccgcgcggtt gagcgtggcg cacagatagg gtgcaccgca attcagttgt tcacaagaaa
    13201 tccgagccag tggttttctc ctcccctttc ggaaaaagac gtgacagagt tcagggatgc
    13261 ggtctcacgg acgaaaatgg ctgcggtagt ggcgcacgat tcttatctca tcaaccttgc
    13321 gagtcccgat gcatcattga gaaagcggtc gattgcagcg atgcttgacg aactcgatcg
    13381 tgcgtcagcg ctgggtatcc cgcttcttgt gacccatctt ggggcgcaca ctggacaggg
    13441 agaggaaaat ggactgcaaa cgctcgtaga tagcctcgac gaggtgactg atgcgcggcc
    13501 ggatgctccg gtttctgtgt gccttgaaac cactgctggt caggggactt ccctcggatg
    13561 ggcttttgaa cacatcgcgt gggttctgtc aaaactgtcg gcaccggggc gattccgaac
    13621 ctgttttgac acctgccacg tatttgctgc gggatacgac ctcgtgactc gcgacacgta
    13681 cgaaaaaacg tggcgagagt ttgataaggt tgtgggaatt aggtatcttg cagtaatgca
    13741 tttgaatgac gcaaaaaaag agcttggcag tcgtgtagat cggcatgcgc acatcggaaa
    13801 gggcgaaatc ggcgaag
//
LOCUS       DAOSOO010000071        13798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4463_length_13798_cov_5.642145, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000071 DAOSOO010000000
VERSION     DAOSOO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_13798_cov_5.642145"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLT86_06825"
     CDS             <1..462
                     /locus_tag="PLT86_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT36360.1"
                     /translation="GKALQIAVDEMESESARVKKLRDKLETTLRDRLDEVFVNGHPTL
                     RLAGNLNMSFAYVEGESLLMGLREIALSSGSACTSATLEPSYVLKAIGVSDDLAHTSL
                     RFGLGRFNTEEEVDYAADRVVEEVNRLREMSPLYEMAKSGVDLKTMAWKRD"
     gene            515..931
                     /gene="iscU"
                     /locus_tag="PLT86_06830"
     CDS             515..931
                     /gene="iscU"
                     /locus_tag="PLT86_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOT36361.1"
                     /translation="MAYSEKVLDHYKNPRNVGSFDPNDENVGTGLVGAPECGDVMKLQ
                     LKINDDGIIEDAKFKTFGCGSAIASSSYATELVKGKTIDEAFAIQNKKIVEELSLPPV
                     KIHCSVLAEGAIKAAVEDWKRRKEAKARENSVPGKQ"
     gene            975..1325
                     /locus_tag="PLT86_06835"
     CDS             975..1325
                     /locus_tag="PLT86_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOT36362.1"
                     /translation="METQTTGATAELVRISDSAAKKILALMAKHPESDGLRIQVRGGG
                     CSGMQYEFSVDKLRERDNVVEHNGAKILIDPKSAIYVRGSDFNYVKNLMEERFEIKNP
                     NVKSTCGCGTSFRI"
     gene            1322..1924
                     /locus_tag="PLT86_06840"
     CDS             1322..1924
                     /locus_tag="PLT86_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT36363.1"
                     /translation="MTPSHDNARAPEIDHDPDSSNYFDVLGLPTRLALNEKDLSDHYY
                     ALSRLYHPDFHQNALPAERLRALRRTAAVNDAFTTLRDPVSRGRWWLEFNGRKLSLAP
                     GLPRELGSLVFDVHDALERFRNAEADITEIERFREVTRDELGRRNKQLAALFGELDEA
                     GVSRADGLLFTRLENLLAEISYLRALTRDIERTLENTGER"
     gene            1921..3768
                     /gene="hscA"
                     /locus_tag="PLT86_06845"
     CDS             1921..3768
                     /gene="hscA"
                     /locus_tag="PLT86_06845"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOT36364.1"
                     /translation="MSRILGIDLGTTNSLIAQVVDGVPHVIRDPQTGERLLPSVVAFT
                     DDGKVLVGQAARDAERNGSGIVISSVKRLMGIGMEHVNEDDCKQFPLTPADSGPVRFT
                     VGNQSMTPPQISAHLLRELRRRAEQALGEPVSDVVITVPAYFNDSQRQATRDAGRLAG
                     LQVLRLVNEPTAASLAYGLDRRDHGTVAVFDFGGGTFDVSILRLQGGIFEVLATNGNT
                     RLGGDDFDRAVARWLLKKAADESGLPEILTDHRVFTQARREAENAKIRLTTESVTVAT
                     LVLPGMDTPYEALLTREEFETTIRATAEQTIVHCREALKDAQINPRDVDSVVLVGGST
                     RVPLVRTLVREFFGIEPLADIDPDEVVALGAAVQADILAGGTKDMLLLDVVPLSLGIE
                     TMGGVMTRLIHRNTKIPCTVVEEFTTPADNVTSIDIHVLQGEREFARDNRSLARFKLP
                     VEPLPAGMARVLVMFLIDANGILSVTAIDEKTKNEHTVEVKPSYGLTDEQVEEMLIDS
                     FDNAERDVTERLLAEAVVAAESILLHTNKALKEGADLLQPEEREGIVRAESTLRKALE
                     STDRQTILDATAALDAATKPLADRLLDRAVNRLLKSRTVDEVLASDETV"
     gene            3799..4116
                     /locus_tag="PLT86_06850"
     CDS             3799..4116
                     /locus_tag="PLT86_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT36365.1"
                     /translation="MPRITFLPADVTVEVSEGTSILDAALDNGISLTHNCGGVGACST
                     CHVIIRDGEDSLSEITQAEDDRLSMAEGLTLHSRLACQAFVGTEDVVVEIPVITSRLS
                     HDA"
     gene            4135..4341
                     /gene="iscX"
                     /locus_tag="PLT86_06855"
     CDS             4135..4341
                     /gene="iscX"
                     /locus_tag="PLT86_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HOT36366.1"
                     /translation="MKLTWDDAEQIAAALAEHHPDVDVLGVHFTDLHRWVVELPGFAD
                     DPKGSSEGKLEAIQMAWLEEVQSS"
     gene            4406..4714
                     /locus_tag="PLT86_06860"
     CDS             4406..4714
                     /locus_tag="PLT86_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT36367.1"
                     /translation="MRQVSAIEVFANTFPEVTHAYRTLKANYRGKGPLDDKTRELIQT
                     AVMVVMNSEEGTRDHARLAAEAGATPDEIIQTVLMVLGPAGITRTSAGLQWVKEALAG
                     "
     gene            4776..5006
                     /locus_tag="PLT86_06865"
     CDS             4776..5006
                     /locus_tag="PLT86_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36368.1"
                     /translation="MRSLFRRPRRKCNYQLTKWIRGEENVQFAVEQLEQHGLPYEIRR
                     SRNGMLSVWTYFDELTGDENESNRLERLLKAS"
     gene            5180..5830
                     /gene="folE"
                     /locus_tag="PLT86_06870"
     CDS             5180..5830
                     /gene="folE"
                     /locus_tag="PLT86_06870"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT36369.1"
                     /translation="MSDEYQRMHPVTDMERDGDYALPPDYERIEKLAYHVSEIMKLLH
                     LDLRDGNLLDTPERVAKLYLEVFSSLDESREPRITVFDNEERYSSMVTVKDIPFYSMC
                     AHHFIPFFGVAHVAYIPTAKIIGLSKIPRIVKFYSRMPQLQERLTEQIANYLDEKLEP
                     RGTYVVMEGRHLCMEMRGIESNGAKTVTSALRGCFAKPEIREEFLDLLKIARWEGH"
     gene            5865..6935
                     /gene="galT"
                     /locus_tag="PLT86_06875"
     CDS             5865..6935
                     /gene="galT"
                     /locus_tag="PLT86_06875"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT36370.1"
                     /translation="MSIVSRLRPRPPYYDPKQWELRWHPLRGEWVVIAAHRDTRPWKG
                     GSVSPPPKEPEYDPTCYLCPGNVRTTGGVNPRYRTTFAFDNDFPPAGTSSPLKTSSHG
                     LYRAARAYGRCRVMCFHPRHDLTLATMDVQEIRRVLSLWQREYRLLGEDPRVKHVLIF
                     ENKGEITGTSNRHPHCQAYGTTFIFSTIQREIQLAKRFLTRTGTPLYRAVLEQELEDG
                     CRIIARRGSVIAFVPYFARYPYEVHVAPLESRPSIADLTGSEANDLAHVLKAVLVRYD
                     NLFTMPFPYVQVLHQAPTDGTHHPEFHFHIELYPPLRNPVTLKYLAGPEIGGGNMLSD
                     VAPEEKARELQVQPEIHYSRRV"
     gene            7135..7725
                     /locus_tag="PLT86_06880"
     CDS             7135..7725
                     /locus_tag="PLT86_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT36371.1"
                     /translation="MSASSTQPIPGTKSLDYLLDSRESRMEMRTATEGVMSGFAPGYT
                     FVFYAFEGFIRLTPGSITPAEFVMVTKADSIRVVGRTRESEKAMVEGILKNEMMETAR
                     YPEIVYRGSNIALDKTGPATYRATIDGTMSQHGVTRRCPIVVDVVVTDTNVRMRGSFT
                     ILQSDYGMRPKRLAGGLVRVKDRVFLSFDLVATRAH"
     gene            complement(7693..8739)
                     /locus_tag="PLT86_06885"
     CDS             complement(7693..8739)
                     /locus_tag="PLT86_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36372.1"
                     /translation="MLALVSGGLDSILAARVIQEQRVEVLPVHFLHPWGCGSWEAVRL
                     ITRQLGVEPRIVEAGQDFIAAVRNPRFGRGRNFNPCVDCRVFMFRSLFPLMAETGSEV
                     IITGEVLGQRPMSQLRKKMILVDREAGLEGRVLRPLSAQRLPPTLPEIANMVDRRALF
                     GFTGRSRAPQRELARRLGVSEFQTPAGGCHLTSPSFVPKLRDFFAHYPDSALTEARAL
                     NLGRHLRISPDLKLIVARNESEGRELEALHDFSEPAEMPRSVYIPDGFDGPTVLACGR
                     VGTEEEELIGGVILRYSRRQNPREPGGFPLRVVSSGNTRRVHARNALPPDIVRRFLIS
                     DVTSSMRPCRDEVE"
     gene            complement(8829..9002)
                     /locus_tag="PLT86_06890"
     CDS             complement(8829..9002)
                     /locus_tag="PLT86_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36373.1"
                     /translation="MRERERELRRQRNRRIKRVKARLREQKAQALTVRNERAQEKQKK
                     RVRRVSSAEEASA"
     gene            complement(9034..9222)
                     /locus_tag="PLT86_06895"
     CDS             complement(9034..9222)
                     /locus_tag="PLT86_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36374.1"
                     /translation="MVLQRLFSVHPPENGPFKPDSVVASEPKRIFVNGKPVDGWIITV
                     KSDEDEVHLMLENIEDVE"
     gene            9570..10259
                     /locus_tag="PLT86_06900"
     CDS             9570..10259
                     /locus_tag="PLT86_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT36375.1"
                     /translation="MKFGKRLQRERERRGLTQRQLGLKVGLTSGTYISQIESGEKIPP
                     LETAIKLANALGLADTVPFVFHAVRERSAVEYGFLSSRIKPPPPGKNEPCPRPLPVRP
                     WASVVEGNLPTGTEGSPADRRCIVLEGSGDADSFAVEIGTDWMADTLCRGDVVVVSPG
                     SEVASGDMALVKVTGKRGTNVEEIKIARAYFRGRWTDLVPGNLSKCPIETYPSHDITV
                     LGKVVAKLSRL"
     gene            10458..11345
                     /locus_tag="PLT86_06905"
     CDS             10458..11345
                     /locus_tag="PLT86_06905"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT36376.1"
                     /translation="MLSGVVPALMTPFTAGGEKYDRNRLRGLCEHLISSRVGGLFVTG
                     TTGEFIALSPEERRQVIEDAVEFAGGRTKIIVHACSYNTMESVALAEFACRIGADGVG
                     SLPPYFHPMDEEAQYAFFSRMCEAVQGKPFYLYNLPAYAANEISLPLIRRLKAAFPHL
                     KGIKDSSGDLERMKNELEMGLPDFQVISGADHRTLDALRLGAKASVTSTANAFPEVFQ
                     AVYDAFNAGDMRRAEEAQERLTALTKILMSGRYLATYKTALRLRGIDIGTVRPPHREL
                     TDTEAHKLTVSLKDMGLIP"
     gene            11414..12292
                     /locus_tag="PLT86_06910"
     CDS             11414..12292
                     /locus_tag="PLT86_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36377.1"
                     /translation="MEDTNMVRVGIVGSDNSHAEAFSKLCNVPQDLTVADAKVVAIFG
                     VDPARTKEVATLGNVPRIVDRPEEMIGSVDAVLVVFRHGGLHRRYAEPFLRAGIPVFV
                     DKPLAISPDDARALLDLAEANNTPLTSFSTLRYSRDMLAFVKEAEEVGPVSLSVFSGP
                     ADRQSEYGGLPFYGIHTVELAQALNGPGVISVHAVENKKNIVATLMYEDGRVTVLNFL
                     GDAAYVFHMLAFGKKGHVGRALDAGTCYKDGLEIVLAMVRTGKRPLERNELLEPVLIL
                     DAIERSLGTGKAVRVV"
     gene            12708..13367
                     /locus_tag="PLT86_06915"
     CDS             12708..13367
                     /locus_tag="PLT86_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36378.1"
                     /translation="MSKAPNPEDTGQNPANSRGRSLRLILGAGLFFFLGVVVGWAFHA
                     ALVTDARLSSGSPGTSSLDQQIVVLRKRIAESERDLELGDDAAPTRILLGNTYFDLGS
                     ALAVSGDSSAAMAAFERAIVHYEKARSAGAASADVLTDLGTMYFRVRRPREAIEAYER
                     AIAADPAHLNAWMNLGVVRKEAFGDTAGARQAWERVLAIDGSGPEAARVRSWLGSTRG
                     E"
     gene            13580..>13798
                     /locus_tag="PLT86_06920"
     CDS             13580..>13798
                     /locus_tag="PLT86_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT36379.1"
                     /translation="MQGKTELAARYDHREVESRWYDFWEQKNAFHAQDVSEKEPFCIV
                     IPPPNVTGSLHMGHALTVTIEDIMIRWHR"
BASE COUNT         3029 a         3878 c         4067 g         2824 t
ORIGIN      
        1 ggcaaggcgt tgcagatcgc cgttgacgag atggaaagtg aatcggcacg cgtgaaaaag
       61 ctgcgtgaca agcttgaaac cacgctcagg gatcgattgg acgaagtctt cgtgaacgga
      121 caccccacgt taaggctggc ggggaacctg aatatgagtt tcgcctacgt ggaaggtgag
      181 tctctgctga tgggactgcg ggagatcgcc ctctcaagcg gatccgcctg cacctcggcc
      241 actctggaac cgtcctacgt gctcaaagcc atcggtgtca gcgacgacct cgcgcacacc
      301 agtctgcgtt ttggtctcgg gcggttcaac acggaagaag aagtggatta tgctgcagat
      361 cgcgtcgtgg aagaggtgaa ccgattgcgc gaaatgtcgc cattgtacga gatggcgaaa
      421 tctggagtcg atctgaaaac aatggcatgg aaacgggact gacaacgcaa cgaaccatca
      481 cgcgttacgc gtgaacgaca caggagtttt ctggatggcc tactctgaaa aggtcctcga
      541 tcattacaaa aacccgcgta atgtggggag ttttgacccc aacgacgaga atgtgggaac
      601 cggtctcgtt ggcgccccgg aatgcgggga tgtaatgaag cttcagctca aaataaacga
      661 cgatgggatt attgaagacg cgaaattcaa gaccttcggg tgcggaagcg caattgcgag
      721 ttcgagttat gccaccgaac tcgtgaaggg aaagactatc gacgaagcat tcgccatcca
      781 gaacaaaaag atcgtggagg aactcagtct tccaccggtc aaaatacact gctccgtcct
      841 ggcggagggc gcgatcaagg cggcagtgga agactggaaa agaaggaaag aagcgaaagc
      901 gcgagagaac tcagtacccg gaaaacagta agttgacatc tggtatccgc acggacccga
      961 aggagtcgca cgaaatggaa acgcagacaa caggtgccac cgcggagctt gttcgcattt
     1021 ccgatagcgc ggcgaagaag atcctcgcgc tgatggcgaa acacccggag tctgatggtt
     1081 tgaggattca ggtgcgcgga ggtggttgca gtggcatgca gtacgagttt tcggtcgaca
     1141 aactccggga acgggacaac gtggtggagc ataacggtgc gaaaattctc atagacccga
     1201 aaagcgcgat ctacgttcga ggttcggatt tcaactacgt caagaacctg atggaggaac
     1261 ggttcgagat caagaacccg aacgtcaagt ccacctgcgg atgtggcacc tcgttccgga
     1321 tatgacacct tctcacgaca atgcccgggc gccggagatc gaccatgatc cggacagcag
     1381 caactacttc gacgtcctgg gacttcccac aaggctcgcg ctgaacgaaa aggatttatc
     1441 tgaccattac tatgcgctga gccgcctcta ccatccggat ttccatcaga atgcgttgcc
     1501 cgccgagaga ctgcgggcgc tgcgtcgcac tgcggcagtg aatgacgcgt tcaccactct
     1561 ccgagatcct gtcagccgcg gccgttggtg gcttgagttc aatggtcgaa aactgtccct
     1621 cgccccggga ttacctcgcg agctgggatc ccttgtgttc gacgtccatg atgcgctgga
     1681 aagattccgc aacgccgaag cagacatcac ggaaatcgag cgcttcaggg aagtaacgcg
     1741 ggatgaactc gggcgtcgga acaagcagct cgccgcactt ttcggcgagc ttgatgaggc
     1801 cggagtttcc cgcgccgacg gccttctgtt cacgcgcctc gaaaatcttc tcgccgagat
     1861 atcctacctg cgggcgctca cgcgggatat agagcgtacc ctcgaaaaca cgggtgaacg
     1921 atgagccgaa ttctcggaat agacctcggt acgacaaaca gtctgatcgc gcaggttgtt
     1981 gacggtgttc ctcacgtaat ccgtgacccg cagacggggg aacgtctttt gccgtcggtg
     2041 gttgcgttca ccgatgacgg taaggtgctt gtcggccagg ccgcacgcga tgcagaaaga
     2101 aatggctccg gcattgtcat ttcttcggtg aaaaggctca tgggcatcgg gatggaacac
     2161 gtgaacgaag atgactgcaa gcagttcccg ctgactccgg cagattcggg gcccgtcaga
     2221 ttcaccgtcg gcaaccagtc catgacgccg ccccaaatct ccgcgcacct gctccgagag
     2281 ctgagacggc gcgcggaaca ggctcttggc gagccggtaa gcgacgtggt gatcacggtc
     2341 cccgcgtact tcaacgacag ccagcgccag gccacgcgcg atgcggggag gctcgccgga
     2401 ttgcaggtgt tgcgtcttgt caacgaaccg accgcggcga gtctcgcgta tggtctggat
     2461 agacgtgacc acggtacggt agcagttttc gacttcggcg gaggcacgtt tgacgtctcg
     2521 attctccgtc tgcagggtgg gatttttgag gtcctggcaa cgaacggaaa tacgagactc
     2581 gggggagatg actttgaccg tgcggtggca cgatggcttc tgaagaaggc agccgacgag
     2641 tcggggctgc ccgagatcct gacagatcac agggttttta ctcaggcccg gcgtgaagcg
     2701 gaaaacgcga aaattcgcct cacgacagaa tcagttaccg ttgcaacgct cgttctcccc
     2761 ggaatggata ctccctatga ggcgctcctt acgagggagg agttcgaaac caccattcgc
     2821 gctacggcgg aacagaccat tgtgcattgc cgcgaagcgc tcaaggacgc gcaaatcaac
     2881 cccagagatg ttgactcggt tgtcctggtg ggcggttcta cgagggttcc gcttgttcgg
     2941 acactggtgc gtgaattctt cgggatcgaa ccgctcgcgg atatcgatcc ggacgaagtg
     3001 gttgctctcg gagccgcagt tcaggccgac atactggctg gcggaaccaa ggacatgctc
     3061 ctgctcgatg tcgttcctct cagtctggga attgagacca tgggcggcgt gatgacgcgg
     3121 ctcattcatc gtaatacgaa gattccctgc acggttgtgg aggagttcac cacgccggcg
     3181 gataacgtca cgagcataga tatccacgtg ctgcagggcg agcgggaatt cgcgcgcgat
     3241 aatcggagcc tggcgcgatt caaactgccg gtcgagcctc ttcctgccgg catggcgcga
     3301 gtgctcgtca tgtttcttat cgacgccaac ggcatcctga gtgtcacagc catcgacgaa
     3361 aagaccaaaa atgagcacac agtggaggtg aagccgtcct acgggttgac agacgagcag
     3421 gtggaggaga tgctgataga ctcgttcgac aacgcggagc gggatgtcac ggagcgcctc
     3481 ctcgccgagg cggtcgtggc ggccgaaagc atcctgcttc acacgaataa ggcgttgaaa
     3541 gaaggtgccg acctgctcca acccgaggaa cgcgaaggga ttgtgcgcgc ggagagcacg
     3601 ttgagaaagg ctcttgaatc caccgatcgg cagacgattc ttgacgcaac cgctgctctc
     3661 gatgcggcaa cgaagccgct tgcggatcgc ttgctggatc gcgccgtgaa tcgtcttctg
     3721 aagagtcgaa ccgtggacga agttctggcc tctgatgaga ccgtgtaggg agcaccaatc
     3781 ggaacagaca ggaccccaat gccccgcata acgtttctac cggccgacgt gacggttgag
     3841 gtcagcgagg gaacttcaat cctcgacgct gcgttggaca atggcatatc gctcacgcac
     3901 aactgcggtg gcgtaggcgc gtgttcaacg tgccacgtta tcatcaggga cggcgaagat
     3961 tcgctgtctg aaatcacgca ggccgaagac gatcggctga gtatggcgga ggggttgact
     4021 cttcactcga gactcgcgtg ccaggctttt gtgggaaccg aggatgttgt ggttgaaatt
     4081 cctgtgatta cctctcggtt gagccacgac gcgtaggttc tcccggagaa tgcgatgaaa
     4141 ctcacgtggg atgacgccga gcagatcgcc gctgcgctcg ccgaacacca cccggatgtg
     4201 gacgttctcg gagtccattt caccgatctg cacaggtggg ttgttgaact ccccggtttc
     4261 gccgacgacc cgaaagggtc gagcgagggc aaactcgagg ccatccagat ggcgtggctg
     4321 gaggaagttc agtccagttg aagacggcgt tcggagtcgc ggaacatttc tcgggaacca
     4381 tttgctaccc tgaggggttt ttacaatgag acaagtgtcg gccatcgagg ttttcgcgaa
     4441 cacgtttcca gaagtgacgc acgcgtaccg cactctcaag gcaaattacc gcggtaaggg
     4501 ccctctggac gacaagacac gggagctcat ccagacggca gtaatggtgg tcatgaactc
     4561 agaagagggc actcgtgacc acgccagact tgccgcggaa gcaggggcga ctccggatga
     4621 aatcatccag accgtcttga tggtgctggg cccggccggc ataacgcgta ccagtgcggg
     4681 gcttcagtgg gtgaaggaag cacttgcagg ctgatgacct gaaaacagaa ccagacgcgg
     4741 gcgccatgtg cgccaaaata caaggtgggt gacatatgag gtcccttttc cgaaggccgc
     4801 ggcgcaaatg caactaccag ttgaccaaat ggattcgcgg ggaagagaat gtccagtttg
     4861 ccgttgagca actggagcag catgggttgc cgtacgaaat acggagatcc cgcaatggga
     4921 tgctctccgt gtggacgtat ttcgacgaac tcactggcga tgaaaacgaa tccaaccgac
     4981 tggagcgtct tctgaaggcg agttgagacc aacgcagcgc caaccgtgaa gacccttcgg
     5041 ttcatccggg tccctagagg gagccggccg tggggtcttt cctttttccc cccgtaaccg
     5101 tacgttactt atgtaacgac aagcgtttgg aggcgggata cgcctccttc ctctgttcgc
     5161 atgcaaaagg cccaccacca tgtccgatga ataccagcgg atgcatcccg ttaccgacat
     5221 ggaacgggat ggcgactacg cgctgccccc tgattacgaa cgaatcgaaa aactcgctta
     5281 ccatgtctcc gagattatga agctcctcca tctcgatttg cgtgatggta acttgcttga
     5341 tactccggaa cgggtggcga agctctacct cgaagtcttc agttcgctgg acgagagccg
     5401 tgagcctcgc atcacggtgt tcgacaacga agaacgatac agctccatgg tgacggtcaa
     5461 ggacatcccc ttctattcca tgtgcgctca ccacttcatt ccgtttttcg gagttgcgca
     5521 cgtcgcatac attccaaccg cgaagataat cgggctgagt aagatcccga gaatcgtcaa
     5581 gttttacagc agaatgcccc aattgcagga gcgactgacc gaacagatcg cgaactacct
     5641 tgacgagaaa ctggaaccga gaggaacgta cgtcgtaatg gaggggagac atctctgcat
     5701 ggagatgcgg ggaattgaat ccaacggggc gaaaacggtg acctcagccc tgcgcgggtg
     5761 tttcgccaaa ccggaaatac gcgaggaatt cctcgacttg cttaaaatcg cgcgctggga
     5821 aggccattga aacgcacgcc aaaggacctg ggaaagggct tctgatgtcc atcgtttccc
     5881 gcctgcgacc tcgccccccg tattacgatc cgaaacagtg ggaactccgt tggcatccgt
     5941 tgcggggaga atgggtcgta atcgcagccc atcgcgatac tcggccctgg aagggcgggt
     6001 ctgtaagtcc cccgccaaag gaaccggaat atgacccgac ctgctacctg tgccccggca
     6061 acgttcgcac aacaggaggt gtgaaccccc gttacaggac gacgttcgcc ttcgacaatg
     6121 atttcccacc cgcagggact tcctccccgc tgaaaaccag ctcccacggc ttataccgcg
     6181 ctgcacgggc gtacggcagg tgtcgggtca tgtgtttcca cccgagacac gatctcacgc
     6241 ttgctaccat ggatgttcag gaaatccgac gcgtcctctc gctgtggcaa cgggaatacc
     6301 gtctgctcgg ggaggatcct cgcgtcaaac acgttctcat tttcgaaaac aagggcgaga
     6361 tcacaggaac aagcaatcgt catccgcatt gccaggcata cggaacgacg ttcatctttt
     6421 ccacgattca gcgcgaaatc cagttggcaa agcgctttct cacgagaacc ggaacaccgt
     6481 tgtaccgcgc ggtattagaa caggaacttg aagacggatg ccggattatc gcgaggaggg
     6541 gaagcgtaat cgcgttcgtt ccctatttcg ccagataccc gtacgaagtt cacgtggcac
     6601 cactcgagtc gagaccttcg atcgcggatc tgacgggttc cgaggcgaac gacctcgccc
     6661 atgtcctgaa agccgttctg gttcggtacg ataacctttt cacgatgccg tttccctatg
     6721 tccaggtact ccatcaggct ccgactgacg gaacacacca ccccgagttc cacttccaca
     6781 tagagctcta cccgccatta cgcaatcctg tcacgctgaa gtacctcgcc ggcccggaaa
     6841 ttggaggggg caacatgctc agcgacgttg cgcccgagga gaaagcgagg gagcttcagg
     6901 tgcaaccgga gattcactat tcccggcggg tttgattgca cggccacgct acgtcgcgat
     6961 cgcgccgcag aagcgaaccc tgcgcctgtg cctgttcgcc gaacctgtga gaccgtccca
     7021 acactcgtcg ctccaggtga gtgtgcggcg aagccccttt tggagtaatc aggtatgcat
     7081 tgccggatgt tcatattgcc tgccgttctg gtggtctttg ctgccggaac atccgtgtca
     7141 gcgtcctcaa cgcaacccat ccctggcacg aaatcgctgg actacctcct cgattcacgg
     7201 gaaagtcgca tggagatgcg cacggccact gaaggagtca tgtccgggtt cgcacccggg
     7261 tataccttcg tgttctacgc atttgaggga ttcatccggc ttacaccggg aagcatcacg
     7321 ccggctgagt ttgtcatggt cacgaaggcg gattcgatac gcgtggtcgg cagaactcgc
     7381 gaatctgaga aagccatggt tgaggggata ctgaaaaacg agatgatgga aaccgcccgt
     7441 taccctgaaa tcgtgtaccg gggtagcaac attgcactcg ataaaacggg gccagctacg
     7501 taccgggcga ccattgacgg aacgatgtca caacacggtg tcacgcgacg gtgccccatc
     7561 gttgtcgacg ttgttgtgac ggacacaaac gtaaggatgc gtggctcctt cacgattttg
     7621 caatctgatt acggcatgcg tcccaaaaga ctcgcaggag gattggtgcg ggtgaaggac
     7681 agggtcttcc tctcattcga cctcgtcgcg acaagggcgc attgaactgg tgacgtcgga
     7741 aatcaggaaa cgacgcacga tgtcgggagg caacgcgttc cgggcgtgaa ctcggcgcgt
     7801 gtttcctgaa gagaccacac gcagcgggaa tccgccgggt tcgcgaggat tctggcgtct
     7861 ggagtaccgc agtatcacac ccccgatcaa ctcctcttct tccgttccga cacgcccgca
     7921 ggcaagaact gtagggccgt cgaacccgtc agggatgtag acggaacgcg gcatttccgc
     7981 gggctcggaa aaatcatgta aggcttcgag ttcccgccct tccgattcgt tgcgcgcgac
     8041 aatgagcttc aaatcaggtg atatccgcag gtgccggccc aggttgagtg cacgtgcttc
     8101 agtcagtgcg gaatcaggat aatgggcgaa gaaatcgcgg agtttcggca cgaaactggg
     8161 gcttgtcagg tggcatcccc ccgccggcgt ttgaaattca gacactccca ggcgtcgcgc
     8221 aagctcccgc tgcggcgccc gcgatcgacc cgtgaaaccg aaaagagccc gcctgtctac
     8281 catattcgcg atttccggca acgtcggtgg aagcctctgt gcggaaagtg gtcgcaggac
     8341 gcgcccttcg agtccggcct ctctgtcgac aaggatcatc ttcttcctta gttgcgacat
     8401 tggccgctga ccgagcactt cgcctgtgat gatcacttcg ctcccggttt ccgccatgag
     8461 cgggaaaagg gatcgaaaca tgaacacacg gcaatctaca cagggattga aattgcgccc
     8521 cctcccaaat cgtgggttcc tcaccgccgc aatgaaatcc tgtcctgctt ccacaattcg
     8581 gggctctacg cccagttggc gggtgatgag acggaccgcc tcccagcttc cacatcccca
     8641 gggatggagg aaatggacgg gcagcacttc aacgcgctgt tcctgaatga cccgcgcggc
     8701 gagaatgctg tccagtccac cggaaacaag tgcaagcatg cggtacgcca ttggtcaccc
     8761 caaaaaagaa ccgtcgagcg gcgcaaaaac ggcgctcgac ggcaatcgcg ttatcaacac
     8821 ggcacgaact acgccgacgc ttcttctgcg gaggataccc ggcgcacccg tttcttctgc
     8881 ttttcctgcg cgcgttcgtt ccgcacggtc agcgcctgcg ccttctgttc cctcaacctt
     8941 gctttcacgc gcttgattct tcggttcctt tgacgccgca gttcacgttc tctttccctc
     9001 atacagcctc cggtatggtt tgaacgtcag gttctattcc acatcctcga tgttttcaag
     9061 catcaaatgg acttcgtcct cgtccgactt caccgtaata atccagccgt ccacgggttt
     9121 gccgttgacg aatattctct tgggttcgct cgcaacgaca gaatccggtt tgaacggccc
     9181 attttccgga ggatgaacac tgaaaaggcg ctgcagcacc atcacagact ccccgcgtcg
     9241 ttctcgttga aaaccctggt acaatgtagc gactgcggtt tcacttacgc aacctcgggc
     9301 aacctggtgc gatgccgtgc cggtttggtg cgatccaggt cattacgtta gccgacacgc
     9361 ggggcggtac gcctcgagca ctcgcctccg ccgcagggat tgcctcgatg cggcataaga
     9421 accgtaattt tggagggtcg ggtggttggc gaaggaactt ccgacactgc tgatcaaacg
     9481 cagaggatgc cgccggtcaa cgcaacgagt tcacacggca ccgtgcctgc ggcccaccgg
     9541 gtcacggcat ctgcccgagg atgaatctca tgaaattcgg gaaaaggctg caacgagaac
     9601 gtgaacggcg agggctgacg caacgtcaac tcgggctcaa ggttggcctg acctcaggga
     9661 cctacatctc ccagattgag tcaggggaga agatcccacc tctggagacg gccatcaaac
     9721 tggcgaacgc gctcggtctg gcagacacag ttcccttcgt cttccacgcg gtgcgggagc
     9781 ggtcggcggt tgaatacggt tttctctcgt cgagaatcaa gccgcctccg cctggcaaga
     9841 atgaaccctg cccgcggccg ttgccggttc gtccgtgggc atcggtggtt gaaggtaacc
     9901 tcccgacggg caccgaggga tcccctgctg atcggcgttg catcgtactg gaagggtcag
     9961 gcgatgccga ttccttcgcc gtcgaaatcg gcacagattg gatggctgac acgttatgcc
    10021 gcggtgatgt ggtcgtggtg agcccgggaa gcgaagttgc cagcggggac atggcacttg
    10081 tcaaggtaac cggaaaacgc ggaaccaacg tcgaggagat caaaatcgcg cgggcatact
    10141 tcagagggcg atggacggac cttgttccgg ggaatctgag caaatgcccc atcgaaacct
    10201 atccgtcaca cgacataacg gttctcggaa aagtggttgc caaactcagt cgtctttgat
    10261 gtctccttct ggtgtggttt ccccgtgccg cgttcgcgtg ctctggtttt ctggtgattg
    10321 agagccgcgg cgccacctgg tgatgctgcg gtcgtctccc gcaaaaggtg gccttgcagc
    10381 ccggggacaa ggcactatat gttcatacaa atcccccagg aatacgaaca cgcaatcttg
    10441 cgaaaggcgc ctttcgaatg ctttccggtg ttgtacctgc tcttatgact cccttcaccg
    10501 cgggcggtga gaaatacgac agaaatcgcc ttcgcgggct ttgtgagcac cttatctcgt
    10561 cccgcgtggg cggccttttt gtaaccggga caactggcga gttcattgcc ttgagcccgg
    10621 aagagcggcg ccaggtgatt gaggacgctg tcgaattcgc gggtggccgc acgaaaatca
    10681 tcgtccacgc ctgcagttac aacaccatgg aatcggttgc gctcgcggag ttcgcgtgcc
    10741 ggattggtgc ggacggcgtc gggagtctgc caccgtattt tcacccgatg gacgaggaag
    10801 cgcagtacgc cttcttcagt cggatgtgtg aagcagtcca aggcaagcct ttttacctgt
    10861 acaatcttcc cgcgtacgcg gcgaacgaaa tcagtttgcc gttgattcga cgtctgaagg
    10921 ccgccttccc acacctgaag ggtatcaagg attcttccgg tgatctggaa cggatgaaaa
    10981 atgaactcga aatggggctt ccagatttcc aggtaataag cggagcggac catcgaacgt
    11041 tggacgccct ccgtttgggt gccaaagcaa gcgtgacatc gacggccaac gcgttcccgg
    11101 aagtatttca ggcggtgtac gacgcgttca acgcggggga tatgcggcgc gcggaagagg
    11161 ctcaggaaag gctcacggcg ctaacgaaaa tcctgatgtc cggccgctat ctggcaacgt
    11221 acaagactgc gttacgcctt cgtggaatag acataggaac tgtccgaccg cctcatcgtg
    11281 aactgaccga tacggaagcc cacaaactga ccgtgagcct taaggatatg gggctcattc
    11341 cctgatcgcg cgggcttgca gtgtcggtcg aattttcccc ggaacagact cgaaaagcgc
    11401 gatggaacac ctcgtggagg acacaaacat ggttcgtgtc gggattgttg gctcagataa
    11461 ttcccacgcc gaagcgttca gcaaactctg caacgttccg caggacctga cggttgccga
    11521 tgcaaaagtc gttgcgatat tcggcgtcga cccggcacgg accaaggaag ttgcgactct
    11581 cggcaatgtt cctcgcattg tggacagacc ggaggaaatg atcggttccg tggacgcagt
    11641 gctggtggtg ttccgccacg gcggactgca tcgtcggtat gccgaaccat tcctgcgggc
    11701 cggcatcccg gtttttgtcg acaaaccgct tgccatctct ccagacgacg cacgcgcgct
    11761 tctggatctc gcggaagcca ataacacccc tttgacgtca ttctctacgt tacgttattc
    11821 gcgagatatg ctggcgttcg tcaaggaagc ggaagaggtc ggccctgtgt cgctctccgt
    11881 gttcagcgga ccggcggacc gccagagcga gtacggtgga cttcccttct acggcatcca
    11941 cacggtggag cttgcgcagg cactgaacgg ccccggtgtg atttctgttc acgcggtgga
    12001 aaacaagaaa aacatcgttg caacgctcat gtacgaagac gggcgcgtga cagtcctgaa
    12061 tttcctgggt gatgccgcgt acgtgtttca catgcttgcc ttcgggaaaa aggggcatgt
    12121 tggccgggct ttggatgcgg gaacctgtta caaagacgga ctggagatag ttctagcgat
    12181 ggtgaggacg gggaaaagac cgctcgaaag gaatgaactt ctggaacccg tactgatcct
    12241 tgacgcaatc gagaggagcc ttggaactgg caaggcggtt cgcgttgttt agtgttgttg
    12301 gcgaatgatg gggtggcccg gcggcgcctg ttgccgctcg agcccccttc cggcggcttg
    12361 gcgcgtggga tagcccccca tcccgcccca agccgctcct ttttttccgg tttcggaccc
    12421 tttgcacggc ccgttccgaa tccggtcatt gaggtagcaa gcaggatgcc aacacgtaca
    12481 aatgccggaa ttccggcatt ttctcggtgc cggcatctca ggcacccacg cagaagtgtc
    12541 tcatactgag acgtgttgcc agcacctacg gtagtagtat cgctgtaagt attttgccca
    12601 actgtattta gtcgatattc tcattttggg aatgggtgag actgcggtca gcgcgggacc
    12661 accaaggcgt tccgccgcgg atgcagcgca gtggaggctc cacaaccgtg tctaaggctc
    12721 cgaacccgga agataccggc cagaacccgg cgaattccag gggccgttca ctccgactga
    12781 ttttgggcgc aggccttttc tttttccttg gagtcgttgt tggctgggct tttcacgccg
    12841 ccctcgtaac ggacgctcgc ctctcctccg gctccccggg cacctcttcg ctggaccaac
    12901 agatagtcgt tcttcgcaaa cggatagcag aatcggagcg ggatctggag ctgggggatg
    12961 acgccgcacc cacgcgcatc ctgctcggaa acacttattt cgatcttgga tccgcgctgg
    13021 cggtttccgg agactcctcc gcggcaatgg ccgcgtttga acgagccatc gttcactacg
    13081 agaaggcgcg ttccgccggg gctgcatccg ccgacgtgtt gacggatctc ggtaccatgt
    13141 atttccgggt gcgcagaccc cgtgaggcca tcgaggcgta cgaacgtgcg attgcggcgg
    13201 acccggctca cctcaatgcg tggatgaatc tcggcgttgt ccgcaaggag gcattcggcg
    13261 ataccgcggg cgcgcggcag gcctgggaaa gggttcttgc cattgacggt tccggtcccg
    13321 aagcagcacg ggtgcggtca tggctcggct caacccgagg tgaatgagga tggtcggcag
    13381 gtcgccttac cccgaaccga ggtgacaggc gtgggctttt gtgcgatatt ttgattgatt
    13441 gtcggtttgc gaggaacacg gactccatgg agttcagctt catgtctacc gtacgcgcgc
    13501 ggcgctgagc cgcgtcccgc ctttttgctt tgaccttctg tccctcccgg acatacagtc
    13561 ccgatcgaaa ggcatctaca tgcagggcaa aaccgaactg gcagctcgtt acgaccacag
    13621 ggaggtcgaa tcccggtggt acgacttctg ggaacaaaag aacgcctttc acgcgcaaga
    13681 cgtctccgaa aaggaacctt tctgcattgt aattccgcca ccgaatgtta cgggcagtct
    13741 tcatatgggg cacgcgctca ccgtgactat tgaggacatc atgatccggt ggcatcga
//
LOCUS       DAOSOO010000072        13614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4537_length_13614_cov_5.788996, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000072 DAOSOO010000000
VERSION     DAOSOO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_13614_cov_5.788996"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2768)
                     /locus_tag="PLT86_06925"
     CDS             complement(<1..2768)
                     /locus_tag="PLT86_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT36380.1"
                     /translation="MNLPRLAIRHHVTTFMAYLLALGFGLFSLSRLKLDLYPDISFPM
                     IMVLTQYRGAGPFDMESVVTEPIESAVSSVEGIKKVTSSSRNGMSVISLEFDWDTDMD
                     MAQMNVSRALDRVTTLPEDVTKPRLFKFSTKMMPIMFMGVRSPVLNQASLRKVCEDYV
                     VPRMKRVEGVASVDVVGGLARQINVEIDPRALASYGFGIETVTAALQRSNMQVPGGSI
                     RDGRTAFAVRTLGEFLSVEQIRDVMLATKNGQVIRLRDVADVSDGFQEVLGEARIDGE
                     SGVMLIVRKQSDANTVQTVRAVKAVLPEITQSVPGNLSLTTVFDQAQYIQDSLGNLSS
                     NALQAFGLTILVLLVFLRNIRSSLIVGISIPVSMVLTFSVMDAAGVTFNVISMAGLAL
                     AVGMLVDNSIVVLENIFRLHELGYDRVEAAEKGSNQVAMAITASTLTTIVVFLPVLFV
                     PGIAGVMFNDMSLTICFALSVSIFVALTAVPSLAAGFLARQHPPRTRFLLRMYEDIGK
                     GLKWLETRYSVVLKWALGHRKTVVFTAAGLFAASIPLFMSIGVNFMEHPDDGRIRCSV
                     QHAVGTDLPTTMATLNMMEEIIKKEVPERTTVYVNAGASGGIGAALQGLDTHSGEIQV
                     KLVPLQDRDRSKSEIQDALRARFDKIPGIKYNFQQDSGPGMGGADIEVRFFGDDLDVI
                     GNLADSTVAIAKKIPGAVDARSNLDRGAPELQIRLDRDRLQAVGLTAGQVSAAVSTAI
                     QGATATRYREAGEEYNIFVRFAEEFRRSPEQLENLIIATPAGALIPLRQVADVRREVA
                     PIAIFRENQGRVATVSIGVSGRDLGSVTKDVEKAISKLNIPNDVIMEVGGGAEDLRES
                     FRYLGLAILAGIVLVYMVMASQFESLVDPLIIGTTVPLSFIGVAIALAITGTTVSVMA
                     LLGMLMLVG"
     gene            complement(2787..3953)
                     /locus_tag="PLT86_06930"
     CDS             complement(2787..3953)
                     /locus_tag="PLT86_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT36381.1"
                     /translation="MEGRIRNHISTHTGDRRWTSALALTAFLAIVAIGSSGCGEKEQQ
                     ADTAEQALPVTVHAPYREDAIRVISANGSLVADKQVTIISPVSGRIVSKRVDLGSRVA
                     ANQVLFVVDYSSLDAAVQQAQAAVSSAEQQAANLATEFARVQRLYAEGGASQSQYDAI
                     RTQKAAADEGVKQARAMLQQAVIRRNDAEVRAPFSGLVGKVFVEVGDMVGPGVPLAVV
                     TEPAPLIAKIQVPERDFGTLRIGQSATVQVASYPNEAFSGTIRQISPILDAMTRMADV
                     EILLPNRDQRLKAGMYARVEIEADRRPNALMVPSNAVLQEASLVAGSTVSNITRTYYL
                     FVAEGERAVRRNVTLGYTTGDRVEITSGIRPDDKIIVQGHSFLQNGQRVSPASE"
     gene            complement(3965..5308)
                     /locus_tag="PLT86_06935"
     CDS             complement(3965..5308)
                     /locus_tag="PLT86_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT36382.1"
                     /translation="MLESIGRTGKVCAFWIVCSMALVSGAGARGISLREAEQMALERN
                     PQIAGAKASLDSARAGITRARATILPSLAATAGYTWNAELPVVVMPAPVGAVSIGEKH
                     NYSLALTARQPVFLGFAGVTGVRMAETALQSSQLRLDATTQTVIASVREAYLAAVLSR
                     MLVTVNEQAVAQAESSLALVQRRADVGSASRFDLLRARVQLATLRPNLTSARSNRDLA
                     DGRLRLAIGVGERDAITPSDTLAVFASEWAKVPLDSLRRIAREQRTEIRQLDIGEQIS
                     NDAVTLSRSAYYPTVAAFATSQWEAQRPSLSVSSGDFRRVSMLGLQLNWTFWDSWKTP
                     ATVQMARVGVRQLNLFRQLATDGISLEVEAAFHGLHEAELNLATGLETVNQAAEALRL
                     ASVMYSEGGNTQLDVISAQLALTSARTQYAQALYNYHVAHIRMEKALGLTRVSQD"
     gene            complement(5372..5974)
                     /locus_tag="PLT86_06940"
     CDS             complement(5372..5974)
                     /locus_tag="PLT86_06940"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT36383.1"
                     /translation="MEPTSHPAVGKETRDRIFAAAAKLFAARGYGSVSMREIADAAGV
                     SKPMLYYYFENKAGLCRALLEEGFQNLSQTWDEIARLDIPLIERFQRVVRAQFRHVRE
                     NPDLVRFHINFLLGPDEAGLAAEFRREHKPIVNILLRMFAEGQASGLFRPDINPSVAI
                     NVFSGAVNIHVGQSILLGGPPLDDDLADQLTQLYLHGVCA"
     gene            6120..8075
                     /gene="uvrC"
                     /locus_tag="PLT86_06945"
     CDS             6120..8075
                     /gene="uvrC"
                     /locus_tag="PLT86_06945"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT36384.1"
                     /translation="MKANQARVTGPVAMANTQKEAAARAEDLRQKVSLLPRKPGVYIF
                     RDSSGEIIYVGKAKVLANRVRTYFADSYDDGRLQIHALVRSIADLEVIATSSETEALV
                     LEATLVKKHAPRYNIRLKDDKKYPYLLLTNEAFPRLVFTRSVRRGEGRYFGPYTDTRA
                     LRRTQELLHRLFPLRTCTSPLPSPSITRVCLQYEIKRCKGPCVGLQTQEEYATVTNQA
                     ERFLRGQISSVVRELEQEMDLASERLQFERAAELRDRLNDIRTTAEKQSLADARGEDR
                     DVVALTRDDTEAVAVVLEIREGKLIARKDYSLSVGLDEPVEAIMGAFLRQYYLDAMAI
                     PRELAVMCEPEDAHALEELLGHQRGGIVRLQTPRRGEKTRLVDMARQNAEMLLAERRL
                     RREKFRGRVPHSVRALERDLRLPRPPRRIACFDISHIQGTDTVGSLVVFQDGRPLKNA
                     YRMFQIRGVSGSVGESDDFAAIREVVERAFSRLAKDTEEARPDLVIVDGGKGQLSSAK
                     AVLDSLGFGDQPVIGLAKRLEEVFLPGCSEPQTIPRTSSGLKLLQQVRDEAHRFAIGY
                     HRKKRGRAMSVSALDEVPGIGIARKRALLRKFRSLASLLAAPEEEIAAVEGIGPVLAA
                     TIRAVLLDGRTGGRNGAQSPPLKVQTS"
     gene            8519..8794
                     /locus_tag="PLT86_06950"
                     /pseudo
     CDS             8519..8794
                     /locus_tag="PLT86_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
     gene            8876..10045
                     /locus_tag="PLT86_06955"
     CDS             8876..10045
                     /locus_tag="PLT86_06955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT36385.1"
                     /translation="MNRLGTENAFEVLAVINRLRAEGRDIVSFAIGEPDFPSPSNVKR
                     AGCSAIMEDKTHYGESAGLRELREAIAAHIRKTRGVDAGPENVVVTPGAKPILFNTIM
                     TLVDPGDEVMYPNPGFPIYESLIDFVGAKGVPVPLWESLDFNFDPEEFRRLVTKKTRL
                     VILNTPQNPTGGILTRDALQVVAEMAQERDFWVLSDEVYSQMAYDGEFLSIASLPGMA
                     ERTVVLDGFSKTYSMTGWRLGYGVMPADVARQEARIETNLNSCTATFTQWAGLEALTG
                     PQDESLAMIAEFKRRRSLIVDGLNRIPGFSCRKPAGAFYAFPNVTEACRMLNKSDSKA
                     LQEYLLFEAGVAVLPRTSFGRRNAGEKDEYVRFSYATSAPLIEEGLERIRKAMEG"
     gene            10160..11170
                     /locus_tag="PLT86_06960"
     CDS             10160..11170
                     /locus_tag="PLT86_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT36386.1"
                     /translation="MPRPVTLFTGQWADLKLADLAPKAKGFGYDGLELACWGDHFNVK
                     EAAGSMQYCDEKKKLLAKHGLKVWSISNHLAGQLVCDPNDERANGFAPPELANKPKKI
                     RDWAIDEMKLTGVAAKNLGVSVVNGFTGSGIWHAFYSFPPWTPGMVEAGYKDFARKWK
                     PILDSFADNGIKFGLEVHPTEIAYDIYTAQAALKAVKNHPAFGFNFDPSHLHWQGVSP
                     SEFIHTFGDRIFHVHMKDAIVKLDGKGSILGSHLNFGDYRRAWDFRSVGRGTVNFEEI
                     IRALNDIGYQGPLSVEWEDSGMDREHGAKESCEFVKKLDFAPSSVAFDAAFEEKGKTA
                     RA"
     gene            11456..12802
                     /locus_tag="PLT86_06965"
     CDS             11456..12802
                     /locus_tag="PLT86_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36387.1"
                     /translation="MTKRFLVHPFVMLGLGVLLIVGAGCESVGLRSMKIYIQQRNWEK
                     ALEQGNIALAENPNDAEAWFAMAQVAAQVDSFDLMLRAIERTSSLTNKHAADFKLMRE
                     AKYGSAFNMGVERYNANDMAGASRFMDIAISIDSTRANAYRLKGMIAQRAGNTQETLR
                     YFDRAYRADTTDVELARVYAQLLDAMGKRNEALSVAHRLYTARPGDKKTVALYLQLLT
                     EAGRFEEALNVVNTALASDPNNAEYNMRAGVLWMQRAQQDVADSTAIVRDLETAAPYF
                     EAAVEADTANTDAAYNLAVCYARLNRRGDAINVMKRLLSVRPNDYQARIQLGTLYVRE
                     ENPDAAEQEFLAVVESIGEPTNAEDRAVASRAYRYLSTIYTYRGAVKQGEADRLRERA
                     QELINESAMARQRTARQQKEREAQALLQQAEPLAAEAQALNQRSVEYDRLSNQYAN"
     gene            complement(12871..13248)
                     /gene="hisI"
                     /locus_tag="PLT86_06970"
     CDS             complement(12871..13248)
                     /gene="hisI"
                     /locus_tag="PLT86_06970"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOT36388.1"
                     /translation="MLDQVKFDDRGLVTAIAQDADNGEILMLAYMNRESLEATLRTGI
                     MTYWSRSRGKLWVKGETSGHTQQVVEMRIDCDGDALLFKVRQKGGACHTGFRSCFYRK
                     LTPDGLVEDGDRVFQAQAVYGTK"
     gene            complement(13263..>13614)
                     /locus_tag="PLT86_06975"
     CDS             complement(13263..>13614)
                     /locus_tag="PLT86_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOT36389.1"
                     /translation="ALLISLGKRRGEALLLGENAAKHRRILGEYPAPFLDVLIVIVAG
                     MTLITYCLFTFHSGHSEKLMLTIPFVLYGVFRYLYLLYVAGSTDAPEVLILNDKPLAA
                     ASILWALLSAALFY"
BASE COUNT         2818 a         3924 c         4149 g         2723 t
ORIGIN      
        1 cccacgagca tcaacatgcc gagaagtgcc atcacgctca cggtggtgcc ggttattgcc
       61 agcgcgattg caaccccgat aaacgaaagt ggaaccgtgg ttccgatgat aagtggatcg
      121 acgagcgatt cgaactggct cgccatgacc atgtacacaa gcacaattcc cgcgaggatg
      181 gccagcccga ggtaccggaa cgattcccgg agatcctccg ctcccccacc gacttccatg
      241 atgacgtcgt tgggaatgtt gagtttgctg atcgcctttt ccacatcttt cgtcacgctc
      301 ccgaggtcgc gtcctgatac gccgatggaa acggttgcca cgcgcccctg attctcacgg
      361 aaaatggcga tgggggccac ttcacgtctg acatctgcga cctgtcgcaa tgggatcagg
      421 gcgcccgcag gtgtggcgat gatgaggttc tccaattgct ccggagatcg ccggaattcc
      481 tcggcgaaac ggacgaaaat gttgtattcc tcgccggctt cacggtaccg ggtggccgtg
      541 gcgccctgaa tggccgtgga caccgccgca ctcacctgcc cggccgtcag gccgaccgcc
      601 tgaagacggt cacggtcgag acgtatctga agttccggcg cccctcgatc gaggttcgat
      661 ctggcgtcga ctgccccggg gatttttttg gcgattgcca cggtgctatc cgcgagattc
      721 ccgatcacgt caagatcgtc tccgaagaaa cggacctcga tgtcggcgcc gcccatgccc
      781 ggaccgctat cctgctggaa gttgtacttg attcccggga tcttgtcgaa gcgagcacga
      841 agagcgtcct gaatttcgga cttcgagcgg tcccgatcct ggagaggaac aagtttgacc
      901 tgtatttcgc cagaatgggt gtcgagcccc tgcagggccg cgccgattcc gccgctggct
      961 cccgcattca cgtataccgt cgtgcgctcc ggaacctcct ttttgatgat ctcctccatc
     1021 atgttgagcg tcgccatggt ggttggcaaa tcggtgccca cggcgtgctg cacactgcaa
     1081 cggatgcgac cgtcgtctgg atgttccatg aaattgacgc cgatgctcat gaacagagga
     1141 atcgacgccg cgaacaatcc cgctgcggtg aagacaaccg tcttgcgatg gccaagcgcc
     1201 catttcaaca caacgctgta gcgggtttcc agccatttca gccccttgcc gatgtcttcg
     1261 tacatgcgga gcaggaatcg tgtccggggc gggtgctgcc gggcgaggaa ccccgcagcg
     1321 agggagggca cagcggtaag tgcgacgaag atggacacgg aaagtgcgaa gcagatcgtc
     1381 agcgacatgt cgttaaacat gacccccgca attcccggaa cgaacaatac gggaagaaac
     1441 acaacgatcg tagtgagtgt cgaggcggtg atagccatcg ccacctggtt gcttcccttt
     1501 tccgccgcct ccacgcggtc gtacccgagt tcgtgcaggc ggaagatgtt ttcgagcacg
     1561 acgatgctgt tatcgacaag cattcccacc gcaagcgcaa gcccggccat gctgataacg
     1621 ttgaacgtga ctcctgctgc atccatgacg gagaacgtga gcaccatgga aaccgggatc
     1681 gagataccta cgatcagaga gctccggata ttccgcagga agaccagcaa tacgaggatt
     1741 gtcaacccga atgcctgcag ggcgttggag gagagattcc ccagggaatc ctgaatgtac
     1801 tgggcctggt caaaaacagt cgtcaacgac agattgcccg ggacgctctg cgtgatctcc
     1861 ggcaggactg ctttcacggc acgcacggtt tgcacggtgt ttgcgtccga ttgcttgcgg
     1921 acgatcagca tcacaccgga ctctccgtca atccttgcct cgcccagaac ctcctggaag
     1981 ccgtcgctaa catccgccac atcacgaagg cggatcacct gtccattttt cgtggccagc
     2041 atgacgtcgc gaatctgctc gaccgaaagg aactcgccaa gggtgcggac ggcgaatgcg
     2101 gtgcgcccat cgcggattga accgcccggc acctgcatgt tgcttctctg aagcgcggcc
     2161 gtgacggtct cgatgccgaa accgtacgat gcgagggcgc gcgggtcgat ctccacattg
     2221 atctgtcgcg cgaggccacc gaccacgtcc accgaggcaa ctccctccac ccgtttcatc
     2281 ctcggcacca cgtagtcctc acagaccttg cgaaggcttg cctgattgag caccggtgaa
     2341 cgcacgccca tgaacatgat gggcatcatt ttcgtactga atttgaacag ccgtggtttg
     2401 gtcacatcct ccgggagagt ggtaacccgg tccagcgcac ggcttacgtt catctgtgcc
     2461 atgtccatgt ccgtatccca gtcgaactcc agcgagatta cggacattcc attgcgcgaa
     2521 cttgaggtga ccttcttgat tccttccacc gaagacactg ccgattcgat gggctcagtg
     2581 acgacggact ccatgtcaaa cggcccggcg cctctgtact gcgtcaggac catgatcatc
     2641 gggaacgaga tatcagggta aaggtccagt ttcaaccttg agagcgaaaa aagcccgaac
     2701 ccgagagcta acagatacgc catgaaggtg gtgacatgat ggcgtatggc gagacgtggc
     2761 agattcatga agggactccc gcggttctat tcggatgccg gtgaaacacg ctgcccgttc
     2821 tgcaggaacg agtgaccttg aacgatgatt ttgtcgtccg ggcggattcc tgaggtgatc
     2881 tccacacggt cccctgttgt gtatcccagc gtgacattcc tccgcacggc cctttctcct
     2941 tctgccacga agaggtagta cgttctggtt atattgctga cggtactccc tgccacaagg
     3001 ctggcctcct gcaacacggc gttggaagga accatcaacg cattcgggcg gcgatcagct
     3061 tcgatttcca cacgggcgta catcccggct ttcaggcgct gatcacggtt gggcagcagg
     3121 atttccacat cggccatgcg tgtcatcgca tccagaatcg ggcttatctg tctgattgtt
     3181 ccgctgaacg cttcattcgg gtacgaagcc acctgaacag ttgcgctctg accgattctc
     3241 agggtcccga aatccctttc cggaacctgg atcttggcaa tcaatggcgc cggttccgtt
     3301 actaccgcca gaggaacacc ggggccaacc atgtcgccta cctcaacaaa tacctttccg
     3361 acgaggccgg aaaacggcgc gcgcacttcc gcatcgtttc tccgaatgac cgcctgctgc
     3421 agcatggcgc gtgcctgttt gacgccttcg tccgccgcgg ccttttgcgt gcggatggcg
     3481 tcgtactgcg actgacttgc gccgccttcg gcgtagagac gctgtacccg cgcaaattcg
     3541 gtggcaaggt tggcagcctg ctgctcggca gaggaaacgg ctgcctgcgc ctgctgaacc
     3601 gccgcgtcca gcgaggagta gtccactacg aagagaacct ggtttgccgc gacacgggaa
     3661 ccgagatcca ctcgtttgct gacaatgcgt ccggaaaccg gcgagatgat tgtcacctgt
     3721 ttgtctgcca ccaggcttcc gttggcggaa atgacgcgaa tggcgtcctc tcggtacgga
     3781 gcgtggacgg tgactggcag cgcctgttcg gctgtatctg cctgttgttc tttttcacca
     3841 cagcccgaag aaccgatcgc gactatcgca aggaacgcgg tcagcgcgag tgcagatgtc
     3901 cagcgtcggt cgccggtatg cgtcgagatg tgatttcgaa tccttccttc cacggtgtgc
     3961 tccttcagtc ctgactgacc cgggtgagac cgagtgcctt ttccatgcgg atatgggcaa
     4021 cgtgatagtt gtaaagagcc tgtgcgtact gggtgcgcgc gctcgtcagc gcaagttgcg
     4081 cggaaatgac atccagttgc gtatttccgc cttcgctgta catcacactg gcgaggcgca
     4141 acgcctcggc ggcctggttc acggtttcga ggccggtggc gaggttcagc tctgcctcat
     4201 gaagcccgtg gaacgctgcc tccacctcca gcgaaatccc atctgtggcc aactgccgga
     4261 acagattgag ctgccggacg cctacccttg ccatctgcac ggttgccggt gttttccacg
     4321 aatcccagaa cgtccagttc aactgcaatc ccagcatgga gacgcggcgg aaatcaccgg
     4381 aactcacact cagtgatggc cgttgtgcct cccactgaga ggtcgcgaac gcggcgacag
     4441 tcgggtagta cgcgctgcgg gaaagtgtta cggcgtcatt ggaaatctgt tcgccaatat
     4501 cgagctgccg gatttccgtc ctctgttccc gcgcgatgcg tcgaagggaa tccagaggca
     4561 ccttcgccca ttcgctcgcg aaaacggcca gcgtgtccga aggtgtgatc gcgtctcgtt
     4621 ctcccacgcc tatcgcgagg cgaagcctgc catcggcaag gtcgcggttg cttctcgcgg
     4681 atgtcagatt cggccgcaga gtggccaact ggacgcgtgc gcgcaggaga tcgaagcgcg
     4741 atgcggagcc cacgtccgcc ctgcgttgaa caagcgcaag gctggattcc gcctgcgcca
     4801 ccgcctgctc gttcaccgtg acaagcatgc ggctcaacac cgccgcgagg tacgcttccc
     4861 ggacggatgc gatcacggtt tgagtggttg cgtcaaggcg gagttgtgag gattgcagcg
     4921 ctgtttccgc catccgcaca ccggtgactc cggcgaaacc gaggaaaacg ggctggcggg
     4981 cagtgagagc gagcgagtag ttgtgttttt caccgatcga aaccgccccg accggcgccg
     5041 gcatcacaac caccggcaat tccgcgttcc aggtgtatcc cgcggtggcc gcgaggctcg
     5101 gcaggatcgt cgcgcgtgcc ctggttatgc ctgcacgggc gctgtcgagg ctcgctttcg
     5161 cgccggcgat ctgcggattg cgctccaacg ccatttgttc cgcctcgcgc aacgaaattc
     5221 ccctggcgcc ggctcctgac accaaagcca tggaacacac gatccagaag gcacacactt
     5281 tgccagtgcg gccgatgctc tccaacatgc aaagtctcct tttcacgcgc ttatgaggca
     5341 ggtaattccg aaggggaaaa cgaagacttc ctcacgcgca cactccgtgg aggtagagct
     5401 gagttaactg gtcggccaga tcgtcgtcca gcggagggcc gccgagaagg atgctctgtc
     5461 cgacgtgaat attgactgct cccgagaaga cgttgatcgc aacgctgggg ttaatgtccg
     5521 gacggaacaa cccgctcgcc tgtccttcgg cgaacatccg taagagaatg tttactattg
     5581 gcttgtgttc acggcggaat tcggccgcca accccgcttc atcaggcccg agcaggaaat
     5641 tgatatgaaa acgcacgagg tcggggtttt cccggacgtg tcggaactgc gctctgacta
     5701 ccctctggaa acgctcgatg agcgggatgt ccaggcgcgc gatttcatcc cacgtctgac
     5761 tcaggttctg gaagccttct tcaaggagcg ctctgcacag gccagccttg ttttcgaaat
     5821 agtagtaaag catgggcttg ctgacgcccg cggcatcagc aatttcgcgc atggagacgc
     5881 tcccgtaccc gcgcgctgcg aataacttcg cggcagccgc aaagatcctg tcgcgggtct
     5941 cctttccgac ggccgggtgg gatgtgggtt ccatgaggcg gttgatctcc gtttctgtgt
     6001 ctacctaccg gtaaggataa taacaaccaa ccacctctcc gtcaagatga gaaccgcgtc
     6061 cagggtacaa caggtaagac ttccaaccgg gacagaatgt tacattttga gggcgcggaa
     6121 tgaaggcgaa tcaggcgaga gtgacgggtc cggttgcgat ggcgaacacc cagaaagagg
     6181 cagcagcgcg ggcggaggat ctccgacaga aggtttccct tctgccgaga aagcccggcg
     6241 tgtacatttt tcgcgattca agcggggaga taatctacgt aggcaaggcg aaggtccttg
     6301 caaaccgggt tcgcacgtat ttcgcggaca gctatgatga cggtcgcctc cagattcacg
     6361 cgcttgtgcg gagcatcgct gacctggaag tgatcgccac cagcagcgaa accgaagctc
     6421 tggttctgga agctactctg gtcaagaagc atgctccgcg gtacaacatc cgtctgaaag
     6481 acgacaaaaa atacccgtac ttgttgctca ccaacgaggc gtttccccgc ctggttttca
     6541 cacggtcggt taggcgtggt gagggcaggt atttcggtcc ctacacagat acccgcgcgc
     6601 tccgccgcac tcaggaactg ctgcaccgtc tttttcccct gcgcacgtgc acgtctcccc
     6661 tgccgagtcc gtcaataacc cgcgtgtgcc tgcaatacga aatcaagcgg tgcaaaggcc
     6721 cgtgcgtggg actgcagacc caggaagagt acgcgacggt gacgaaccag gcggagcgat
     6781 tcctgagagg gcagatttcg agtgtcgtga gggaactgga acaggagatg gacctcgctt
     6841 cggaaaggct ccagttcgag cgcgccgccg aactgcggga ccggttgaat gacatacgga
     6901 caaccgcgga gaaacagagc ctggcagatg cccgcgggga ggacagggat gttgtcgcgc
     6961 ttacgcgcga cgacacggaa gcagtcgcgg ttgttcttga aatccgggaa ggcaaactga
     7021 ttgcccggaa ggactattcc ctgtcggtcg gtctggatga gccggtcgag gcgatcatgg
     7081 gggcattctt gcgccagtac tacctcgacg cgatggcgat accgcgggaa ctcgcggtga
     7141 tgtgtgagcc ggaggatgcg catgcccttg aagaactgct gggccatcag cggggaggga
     7201 ttgtccgcct gcaaacaccg cggcgcgggg aaaagacccg gcttgtcgac atggctcgac
     7261 agaacgcgga gatgctcctt gctgagcgcc gcctgcgccg ggagaaattc cggggccggg
     7321 tgccgcactc ggtgcgagcg ctggagcgcg atctccggct tccccgtccg cctcgcagga
     7381 tcgcgtgttt cgatatttcg catatccagg gcacggacac ggtcggctct cttgtggttt
     7441 ttcaggacgg acggcctctg aaaaacgcgt atcggatgtt ccagattcgg ggcgtcagcg
     7501 gaagtgtcgg ggaatccgac gacttcgccg cgatccggga agtagttgaa cgggcattct
     7561 cccgtctggc gaaagacacg gaggaagcaa gaccggacct cgtgatcgtg gatgggggaa
     7621 aaggtcagct ctcaagtgcc aaggcggtgc tggattcact gggtttcggt gaccagccgg
     7681 taattggtct cgcgaagcgg ctggaagagg tttttctgcc gggttgcagt gaacctcaga
     7741 ccatcccccg gacgtccagc ggattgaaac ttctgcagca ggttcgggat gaggcgcacc
     7801 gcttcgcaat cgggtaccac cgaaaaaagc gcggcagggc aatgtccgta agcgcgctgg
     7861 acgaggtgcc ggggatcgga attgccagaa agcgtgcgct gttgagaaaa ttcagatcgc
     7921 tggcgagtct tctggccgcg ccagaagagg aaattgcggc ggtggaaggc atcggcccgg
     7981 ttctggcggc tacgatccgg gcggtgctgt tggacggcag aacgggcggc aggaacggcg
     8041 cgcagagtcc gccgctcaag gtgcagacga gttgacccgg ggcttcgcgc aggcgttaat
     8101 tcagatagcc ggatggcgcg caatcagacg ccgtttcgca gaaactacga gaagcattgc
     8161 ccggacgcat gggcggtgct ccgattttca gaagtgacgt acggtggcag ggaacgtcga
     8221 atctactcga gttgcagccg aacgcgcggc gaagcgcctt cggccgtatg ctttccttca
     8281 ggcaggcgac actctgcgca aatgtctgga cggattcccg agtggcgccg tggcaccttt
     8341 gatcatcaca actgctgcct gtgaggggat tccggaggat ttcagggttt ttgttccggc
     8401 agatgcgata gaagcggaaa aagcagtcat ctcatcgggg cttcggcctg cggtgattgc
     8461 agtgaccgac tcaccagttc ccgcgtgcac cgcactctca gacgttctcg aaatcgggat
     8521 gccaaacctg taccgcgaag ggcaggacgg cgcgatagtg acctccgggc cggctcttca
     8581 tgtggcgctg gaggttgccg atgaggctac ccgaaacggg ttgtcgctga cagtgatcca
     8641 cctgcccacg ctctctcctt tgaaccgcgc ggagctgcgc cgccttctgc cgtctcgcgc
     8701 acccgttgtg tttgtttcac cgcatcctct cgcgcacacg gtcctggaaa gcctccagag
     8761 tgaggttccg ggatcgcgaa tcgctgaagc gacggcacct gcgttaacca gagcgctcgc
     8821 agacgctttg cctgaaggag ccggatctgt gggcaaaacg cgattcgcgg aacggatgaa
     8881 ccgtctcggc accgagaacg cgttcgaggt tctcgcggtt atcaaccggc tgagggctga
     8941 ggggcgggat attgtaagtt tcgcgatcgg cgaacccgat ttcccgtctc cttccaatgt
     9001 caaacgagcc ggctgcagtg ccatcatgga agacaagacg cattacgggg agtccgcggg
     9061 attgcgggag ctccgggagg caatcgccgc ccatatccgg aagacgcgcg gcgtggatgc
     9121 cgggccggaa aacgtggtgg tgacaccggg ggcaaagccc attcttttca atacgatcat
     9181 gaccctcgta gaccccggcg acgaggtgat gtacccgaat cccgggttcc caatctacga
     9241 atcgctcatc gattttgtcg gagcaaaggg cgttcccgtg cccctctggg agtcgctgga
     9301 cttcaacttc gatcctgagg agtttcgccg tctcgtcaca aaaaagacgc gcctggtgat
     9361 actcaacacg ccacagaatc ccacgggggg aattcttacg agggacgcgt tgcaggttgt
     9421 cgcggagatg gctcaggaac gcgatttctg ggtcctttcg gatgaagtgt attcgcagat
     9481 ggcgtacgac ggggaatttc tgagcattgc atccctgccc ggcatggcgg aacgcactgt
     9541 ggttctcgac gggttctcca agacgtattc aatgaccggc tggcgcctcg gatacggggt
     9601 aatgccggcg gacgttgccc ggcaggaggc tcgcatcgag acgaacctca actcgtgtac
     9661 ggccacgttc acccagtggg cagggctgga ggcgttgacg ggtccgcagg acgaatcgct
     9721 cgccatgatc gccgagttca agcggagacg ctcgctgatt gtggacggtt tgaaccgtat
     9781 ccccgggttt tcgtgtcgca aaccagcggg tgcattctac gcgtttccca acgtcaccga
     9841 agcgtgccgc atgctcaaca agagcgattc gaaggcgctg caggagtatc tgctgttcga
     9901 ggcgggcgtt gcggtgctgc cccggacgtc gtttggaagg cggaacgccg gggagaagga
     9961 cgaatatgtt cggttcagtt acgccacgtc cgctccgctg atcgaggaag gattggagcg
    10021 gatcaggaag gcgatggagg gttaagtaga accggttgag gacttggtgt gtgccagatc
    10081 agttggcgaa taatgcggtt ctcggtgtaa tcacgtccgt ttcagttcca tgagttccat
    10141 acccggggag ggcagaagga tgccacgacc agtaacactg tttacaggac aatgggccga
    10201 tctcaagctg gcggatcttg cgccgaaggc gaaggggttc ggatacgacg gtcttgagct
    10261 tgcgtgctgg ggcgaccact tcaacgtgaa agaagccgcc ggaagcatgc agtactgcga
    10321 cgaaaagaag aagctcctgg cgaaacatgg gctgaaagtc tggtcgattt ccaatcacct
    10381 tgccggccaa ctcgtctgcg atccgaacga cgagcgcgcg aatggctttg cgccgccgga
    10441 actggcgaac aagccgaaga agattcgcga ctgggcaatc gatgagatga agctgacggg
    10501 agttgcggcg aagaacctcg gtgtgtcggt tgtcaacggt ttcaccggct cgggaatctg
    10561 gcatgcgttc tacagcttcc cgccgtggac tccggggatg gttgaggcag ggtacaaaga
    10621 ttttgcgcgg aaatggaaac cgatactgga cagcttcgcc gacaacggaa tcaagttcgg
    10681 tcttgaagtt cacccgacag aaatcgcgta tgatatctac accgcgcagg ccgctctcaa
    10741 agcggtgaag aatcacccgg cgttcgggtt caactttgac ccgagccacc tgcactggca
    10801 gggcgtgagc ccctcggagt tcatccacac gtttggcgac aggattttcc acgtgcacat
    10861 gaaggatgcc atcgtcaaac ttgacgggaa agggagcatc ctcggctcgc atctcaattt
    10921 cggggattac cgccgggcct gggatttccg gagcgtgggc cggggcacgg tgaacttcga
    10981 agagatcatt cgtgcgttga acgacatcgg ctaccagggg ccgctttcgg ttgaatggga
    11041 agacagcggc atggaccgtg aacacggcgc caaggaatcc tgtgagttcg tgaaaaagct
    11101 ggatttcgcc ccttccagcg tcgcatttga cgcagcgttt gaggaaaaag gcaagacggc
    11161 cagggcatga tcggagaaac gtccccctct tccgcccttg cgcggaggag ggggacacgc
    11221 atgtacagtt gacgatttcc tcattatccc gtacttttta aaattcctat gtatcccccg
    11281 cggcacgaga caccccacct gtttgcgtga tgcccgggcc tttggaatga tgtatgcttc
    11341 cctgtacgga agactacttc gggcgtttcc gacgacacga acgtccgcta cttctgccgg
    11401 aagctcccgg ttgagcttct tcacattccg tctcatgtcc tggaaaggat tatccatgac
    11461 caaacgtttc ctggtgcatc cgtttgtgat gctcgggctc ggggtattgc ttatcgtcgg
    11521 cgccggttgc gaatccgtgg gtcttcgttc gatgaagatc tacattcagc agcgcaactg
    11581 ggaaaaggcg ctcgaacagg gcaacatcgc gctggcagag aacccgaacg atgcagaagc
    11641 atggtttgcc atggcgcagg tggctgcgca ggtcgattca tttgacctca tgctgagggc
    11701 gatcgagcgg acctcatcgc ttaccaacaa gcatgccgcg gatttcaaac tcatgcgcga
    11761 ggcgaaatac gggtcggcgt tcaacatggg agtcgagcgc tacaacgcca atgatatggc
    11821 gggggcctcc cggttcatgg atatcgctat ctcgatcgac agcacgcggg caaatgcgta
    11881 ccgcctgaag ggaatgatcg cccagcgggc cgggaacacc caggaaacgc tccggtattt
    11941 cgatcgtgcg taccgggccg acaccactga cgtcgaactg gcacgcgtgt acgctcagtt
    12001 gctggacgcc atgggaaaga gaaacgaggc tttgtcggtt gcgcaccggc tctacaccgc
    12061 gcggccgggc gacaagaaaa cggtggccct gtaccttcag cttctcactg aagccggccg
    12121 ttttgaagag gctcttaacg tggtcaacac cgcgctggcg agcgatccga acaacgccga
    12181 atacaacatg cgggcaggcg ttctgtggat gcagcgcgcg cagcaggatg ttgctgactc
    12241 caccgcgatc gttcgtgacc tggaaacggc agccccgtat tttgaggctg cggtggaagc
    12301 agacacggca aacactgacg cggcgtacaa cctcgcggtg tgctatgccc gccttaaccg
    12361 ccgcggtgat gcgatcaacg tcatgaagcg gttgctcagc gtcagaccga acgattatca
    12421 ggcgcgcata cagctgggaa cgttgtacgt ccgtgaggag aacccggatg ccgcggagca
    12481 ggagtttctg gcagtggtgg aatcgatcgg cgaacccacg aatgcagaag atcgtgccgt
    12541 tgcatctcgc gcgtaccgct acctcagcac catctacacg taccgcggcg cggtgaaaca
    12601 gggtgaagca gaccgcttgc gggaacgggc acaggagctg atcaacgagt ctgcaatggc
    12661 gcgccagcgg actgcccgtc agcagaagga acgagaggct caggctcttc tgcagcaggc
    12721 cgaacccctt gccgctgaag cgcaggcact gaatcagcgc agcgtcgaat atgaccgttt
    12781 gagcaaccag tacgcaaact gagctgcgaa ccaacgaaaa agagggaggg ttcccggtgc
    12841 gggtcccctc cctctggttt ttccccgttt ctacttggtt ccgtacaccg cctgcgcctg
    12901 gaagacgcga tcgccgtcct caacgagccc atcgggagtc agtttccggt agaaacagct
    12961 gcggaagccg gtgtggcagg cgccgccttt ctgacggact ttgaacagga gcgcgtcgcc
    13021 gtcgcagtcg atcctcattt cgacgacctg ttgtgtgtgg ccggaggtct cccccttcac
    13081 ccagagtttt cctcggcttc tgctccagta cgtcatgatg cccgtgcgca gagtcgcttc
    13141 cagcgactcg cggttcatgt atgcgagcat caggatttct ccgttgtcgg cgtcctgagc
    13201 gatagccgtg acgagccccc ggtcgtcgaa tttcacctgg tcaagcatcg ttgtttcccg
    13261 aatcagtaga agagcgccgc ggagaggagt gcccacagaa tacttgccgc ggcgagcggt
    13321 ttgtcgttga gaatcagcac ttcgggcgcg tccgtcgagc cggcgacgta caggaggtag
    13381 agatagcgga acaccccgta caacacgaag ggaatagtaa gcatgagctt ctcggaatgt
    13441 cccgaatgaa acgtgaacag gcagtacgtg atgagggtca ttccggcaac aatgacaatc
    13501 aggacatcaa ggaatggtgc cgggtactcg ccaagaatgc gccggtgttt cgccgcgttc
    13561 tctccgagaa gcagcgcttc tccccggcgc ttgccgaggc tgatgagcaa tgcg
//
LOCUS       DAOSOO010000073        13495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4588_length_13495_cov_5.184449, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000073 DAOSOO010000000
VERSION     DAOSOO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13495
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4588_length_13495_cov_5.184449"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLT86_06980"
     CDS             complement(<1..629)
                     /locus_tag="PLT86_06980"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOT36390.1"
                     /translation="MKSRSLRFELTFWYSTVMAVTLLVMGVAVEQVAYNRISASIDNS
                     LRQAAFTVISEVGVLRSKPDAEPFGETSTVLNPPPWPPRYVQILSASGEVLYRSANLG
                     QYPLPIDSAALRDRGRGMVISPDMRLHGEPIRMITFPLPEYDGSDAAWGQVALSLHDL
                     DRARKKNQLALAIILPAAIGISAVAGWWLARRALRPIDGVIRTAQRIRAA"
     gene            complement(626..1297)
                     /locus_tag="PLT86_06985"
     CDS             complement(626..1297)
                     /locus_tag="PLT86_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT36391.1"
                     /translation="MRVLLVEDDSRIAHFIQKGLTESGYVVDLVADGVEAVSQVASTA
                     YDVIVLDVMLPRMDGFGVLESLRQQGQKMPVLMLTARDTTDDKVRGLDAGADDYLTKP
                     FSFAELEARLRALLRRGTGEASAHLRLGDITVDPVSHVVKRGDTVIEMTPKEYAVLEY
                     FMRYPGRVLTRTMIIEHVWQYQFDTDTNLVDVYINRLRRKLNDAEGRILQTIRGVGYT
                     LRPAQ"
     gene            complement(1625..>2868)
                     /locus_tag="PLT86_06990"
     CDS             complement(1625..>2868)
                     /locus_tag="PLT86_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36392.1"
                     /translation="GKPVEARRRDATDFWKYGRLDPDSNVLFGEGGAGAFSDGKLTSR
                     SKDWRRDDVLDRLARCGAGDSVRFEARPHVGTDRLFYVLKEMRATLSALGTEILFETR
                     VEDFLIDRGTLLGVRCAPDVVIPTNALILACGHSARDTYHALRARGVALEQKPFAMGI
                     RVQHRQEIIDRAQYGRRAVSHEGRDRSLQPAEYVLRCPQTSTGRSVFSFCVCPGGAVI
                     PCASEPGGLFCNGMSGRNRGGRFANGAIVAQVPTADFASADPLSGIDFQRHWERAAFK
                     STGGTYALPMMPLVSFVDGSWPPRRVHGLAKGLFPRADLVDLTSLLPPSVVQAVREAS
                     HEFSKTIHGWLAPDAVAFGVETRTSSPVRILRREDGQSENVAGLFPAGEGAGYAGGIV
                     SAAVDGMRAAEHLMATFAPPS"
     assembly_gap    2869..2968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2969..3399)
                     /locus_tag="PLT86_06995"
     CDS             complement(<2969..3399)
                     /locus_tag="PLT86_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOT36393.1"
                     /translation="MLVLSLSDISVPFGESEDALRALCAARLGVPEAELGQLRVRRRA
                     LDARHPSRLRYVYTIEVEAPLTLEQARRISGAKVSASAPERRVEFGTRALLERPVVVG
                     AGPAGLFAAWKLAENGYRPILIERGKPVEARRRDATDFWKYG"
     gene            complement(3377..3949)
                     /locus_tag="PLT86_07000"
     CDS             complement(3377..3949)
                     /locus_tag="PLT86_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36394.1"
                     /translation="MCGMTGHEHRGVRENSRRRLDPVEEKILLLLAGRAEDMVPLVDI
                     TTDLAGLPDHLRGQWFDKKLLTPSQVAAWEEALKQVRRAVRSLFRKGFIDVGREDEFP
                     EGFCRHHAEVIRALQNHPAWKGDTDTSKGSVAGIENEIRARMPWADARDLTAVQKQMD
                     GPVDIVGITSSGRAELTSNPGQSLAGSQPF"
     gene            complement(3960..4994)
                     /locus_tag="PLT86_07005"
     CDS             complement(3960..4994)
                     /locus_tag="PLT86_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOT36395.1"
                     /translation="MKWEMVVNPETIVGEAHASTLIESPGGDLLCAWFGGAPEGSPDT
                     AIWMSRRTSSGWSPASVIADEPGVPCFNPVFFRHRTTIWVYFKAGKEVEKWSGLRTRS
                     EDGGFTWSAPEILPAGLFGPIKNKGIRLHNGMIAFPSSAESYRAWTGWVYLHDPDSDA
                     WKIRGPLAHPDNPWGVIQPTLWEVTPGHIRALLRSRKEIGRVCVSDSFDSGETWTPAR
                     PSSLPNPNSGIDAVKLADGRVVLAYNHTDPSPRLSAATGDMPGNTPLLGGRHEIHLAV
                     SEDGGETWSPPWEMEIGGAEFSYPAIIQSSDGLVHVTYTWNRKGIRYGAFTPAEISLI
                     CAGTFPRPAP"
     gene            complement(5036..6100)
                     /locus_tag="PLT86_07010"
     CDS             complement(5036..6100)
                     /locus_tag="PLT86_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOT36396.1"
                     /translation="MPDQKIDVVRMSFEEERASAYLYRAMESVETGAAAAMFAKLRKA
                     SEAQSEIWAEQLSSLGATVPAFRPSLRVRVVQWLLFRLGPRRMLPVLAGMKVRGLSIY
                     GTRGATPSTVEEALASPSTAGEKWHRAAQGGGALRAAVFGMNDGLVSNASLIIGVAGG
                     AADQPEVVLLAGVAGLIAGGFSMAAGEYISVATQKELYEHQIALEKAEMAVFPEEEIE
                     ELAMVYETKGLPRDQAVAVATRIVNDPVNGLDTLAREELGLDPAGLSSPVAAASASFA
                     AFAVGALIPLMPYLVTRAPATLSLTVVVTEAALLLVGAVMSLFTGRNMLWSALRMALI
                     GSAAAAITFAIGRLLGVSIS"
     gene            complement(6112..7122)
                     /gene="thiL"
                     /locus_tag="PLT86_07015"
     CDS             complement(6112..7122)
                     /gene="thiL"
                     /locus_tag="PLT86_07015"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT36397.1"
                     /translation="MNADGMTEADLLKTLFGTLPPCGADVIVGVGDDCAVLRADGDDV
                     QVLTTDALIEGVDFVVGPGFSFENLGHKALAVNLSDIAAMGAIPRHALVTLCIPEHYG
                     RKEMAELYQGFSRLASRHGVGVIGGDLSSSRNDFMVSVTLLGTASRSEILLRSGAKPG
                     DAAMLFGRIGDSGAGLDITRGAHCPSEAILLERHFRPEPLVDQGRWLANRTGNHVHAM
                     IDISDGLASDARHICEASGVGVRIDHASLPVSDELAEFARYTGRELDGYLLGAGEDYA
                     LFAAVDASAATEIAGRFRSAFPRTPVATVGSFRRGNHVEIRWENGAVSELAGGFDHFA
                     RQ"
     gene            complement(7139..8389)
                     /gene="lysA"
                     /locus_tag="PLT86_07020"
     CDS             complement(7139..8389)
                     /gene="lysA"
                     /locus_tag="PLT86_07020"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT36398.1"
                     /translation="MHYFAYANEVLRGEEVDLRDIAESVGTPCYVYSHRTLLQHAHVL
                     DAAWGEREHLTCYSVKACSNIAILRLFAQMGLGADIVSGGELYRALLAGFPPDKIVYS
                     GVGKTTEEMREALRAGIFCFNVESEAELVELNRVAQREYRRARISLRINPEIDPKTHP
                     KTATGLKTAKFGVPHSRAVETYRRASELEWLEVIGIDAHIGSPIMSVQPFVDAAKRLV
                     DLVGHIRAEGIELKWIDIGGGLGITYDVEDPPTPKEWAAAVGDVLNDAGLGIITEPGR
                     SIVGNAAVLLTKVLYLKRNEDKNFVVVDAAMNDLIRPSFYDSFHRIQPVVQRGTEPFV
                     ADVVGPVCESGDFLARNRSIARPEQGDILAVMSAGAYGFAMSSQYNSRRRAAEVMVKG
                     GTWQVIRDRETCEDLVRGERFFTL"
     gene            8548..8748
                     /locus_tag="PLT86_07025"
     CDS             8548..8748
                     /locus_tag="PLT86_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36399.1"
                     /translation="MSYRQFAEMAELHRRLVGQVDQRVGKEKAILRKYIAAIDVALRN
                     HPDANEFFARWRKESGMSEREA"
     gene            complement(8812..12072)
                     /locus_tag="PLT86_07030"
     CDS             complement(8812..12072)
                     /locus_tag="PLT86_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT36400.1"
                     /translation="MPDFSVIAKNFADALIAYGTDHYGTRDTPLFCHFIDLETLTVPA
                     QKTAAEWRDLMADWEEDKGYLMWGKDRSNLTWAHQSNLLWDTETVRLLIKLSESTGDR
                     RYAQAAWSCMAYFPEHCVSRETGLFAWGEHVAYNVVSDRIEGERHELQHADPLWEELW
                     QISPTKVQREIEAIYRFHVVDKAAMIYDRHARFWDGLPERDQATILGYAGVFAKAWAF
                     LWTKTRNPEYLRWARNQLLAFQSKSNGAGLYPDNWTDSDKRQLPRNYPARPGLARDMF
                     DIYVMTGMREWLDDGCRYLEACVGLRHEESRTMETKGDPVFGSDVAAGIEVLISAFNL
                     TRNQQWRDLAEEYGRTLAAEPIPTPLMASRLAERIQAFCALHAATGDPRWLSCATRDA
                     EYAITTFVHSSGLIKGTAPVRRRDYYDAIQGSGKLALALYSLGELIRTEGNANQERRE
                     AAAVPDTIPPSVDVLSPPAKLPNHLPTVVQAEIRHADGIQRAVLHYSVGYRVGLADCT
                     PLRETSVFRFHIPPFAPFEGEVRFAVEAFSGGSPQSRTITAWHTFSVRTVENLVADFA
                     GCAAGERTPVRVEGLLPGDSVTVETEPRESAGDRILPTPWTRAPLLIRVSAPHRESAQ
                     IVIGYSARHVDRLIEDTVRPAVLDEKSGAWRILTSASVNTRARTITCQFVQSGLWTIC
                     GLSRVRWQAPQRESNATVGDIDGDGRLEVALTQYANAELLNSDGSSRHVFQLQASNRP
                     VQNTSSPLIADINADGRNEIVYGATSGHVHAFSPDGVELWRTPVGGEVRGGIAAAMSD
                     DGRPSCLCVSWHDAGVALLNTDGSVRWQHSLTPPADTTPVVCIPRAGGPFVVVAHGEG
                     LIAFRCADGEVLWQYRTEGGSPVAPAVGEFSRSEEPHIVTGTASGMIYVLDAHGEVRS
                     RWVVPAVHEAYRPIIEVGMASLRDSSTRQIVATTAGGVVVAYERDGTPVWQFVSREQM
                     TGFALGVGGRLAFADVNADGRYEVLVAEQDQYVYSLKGDGSLLWEFRGAFFYHYSPVV
                     ADLDGQGDLMLLTTSPCQNGTYALRAGHRVAGASRTPWPTMRGNFARTNCAPWPHS"
     gene            12053..13408
                     /locus_tag="PLT86_07035"
     CDS             12053..13408
                     /locus_tag="PLT86_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36401.1"
                     /translation="MTEKSGIPHLSRWHPVSFWPLCSDFQYQERKENIGETNSRPSAR
                     AGFSLGAAFRLLHTLLSVPATWKSFSHSAVFWLREPGFPLEEFMYRHIPLRHPRPTAQ
                     RFIDGLMGRFKETKPRMVEYLVDGSVMKPIATSLLGREWVDTPTDAESRRKALDIAIE
                     FWYRMGYDFIRIERGLVFPKHSLRADDVSGVPGNQRTWADEHQGMIRTWEDFEKYPWP
                     RLEDCDFFDLEYVSRNLPDGMGLIASHGGGPFEQLSQIFSLEGLALALYDDPDLVKAV
                     NDKIGNLMTGFYRHLVQLDNLVAVFPGDDMGFRTGTMIAPAHLRAYTLPVHTRVAEMA
                     HERGLPYFLHSCGNVLPIVEDVINDVKLDGKHSFEDAIIPIQDFQREYGEKLAVLGGV
                     DIDLLAAGSVQDVRKHVRFLMETCGARGRFALGSGSSIPNYVPVANYLAMLDEAIDFS
                     S"
BASE COUNT         2630 a         4067 c         4043 g         2655 t
ORIGIN      
        1 gcggcacgta tccgctgggc agtcctgatc acgccgtcga taggccgaag tgcgcgtctg
       61 gcgagccacc atcccgccac agccgaaatg ccaatcgcgg cgggaaggat aatcgcaagc
      121 gccagttggt tcttttttct ggcgcggtcc agatcgtgga gcgaaagtgc aacctgtccc
      181 cacgcggcgt cgcttccatc gtactcgggc aacggaaacg tgatcatgcg gatgggttcc
      241 ccgtgcagac gcatgtcggg agaaatcacc atgccacgac cacggtctcg caacgcagcg
      301 gagtctatcg gaagcggata ctgccccaga ttggccgaac ggtagagaac ctctccggat
      361 gccgaaagaa tctgcacgta gcgcggcggc cacggcggcg gattcagcac cgtactggtc
      421 tccccgaaag gttcggcatc gggtttcgaa cgaagcacgc ccacttcact gattacggta
      481 aatgcggcct gtcgcaacga gttatctatg ctggccgaaa tccggttgta cgccacctgt
      541 tccacggcga cacccatgac gagcagggtt actgccataa ccgtcgaata ccagaacgtc
      601 aactcgaatc gcagacttcg gcttttcact gtgccggcct caacgtgtac ccgaccccgc
      661 ggattgtctg gagtatgcgc ccttccgcgt cgttcaattt ccgccgcagc ctgttgatgt
      721 atacatcaac gaggttcgtg tcggtatcga actgatattg ccacacgtgc tcgattatca
      781 tggtccgtgt cagaactcgg cccgggtacc gcatgaagta ctccagaacg gcgtattcct
      841 ttggtgtcat ttctatcact gtatcgccgc gcttcacgac gtggctgacg gggtccaccg
      901 tgatatcgcc caggcgcaga tgcgcgcttg cctcgccggt tccccttcgc agaagcgccc
      961 ggagccgtgc ttccagttca gcgaaagaga acggtttcgt cagatagtcg tctgcccccg
     1021 catccagccc gcgaacctta tcgtcggttg tgtcgcgcgc ggtcagcatg agaaccggca
     1081 tcttctgccc ttgctgcctc agggattcca gtacgccgaa tccgtccatt cggggcagca
     1141 tgacatccaa aacgatcaca tcatacgcgg tggaggcgac ctgcgacact gcttcgacgc
     1201 cgtcggccac cagatctacc acgtacccgc tttccgtcag gcccttttga atgaaatggg
     1261 cgattcggga atcgtcttcc acgaggagta cgcgcacggg gactcccctt tcgctggaaa
     1321 agccgcggac ggcgaacccc gcttcgggag gccgcgaaat ccgcagttgt gcccgtattt
     1381 ccgaaatcac ctgcgagttc tccttcttac cccagaaaat ctatcccggc aatatgaaga
     1441 ggaactgaag cagtgatgaa actttgttca tctagccttc tgcgcgacca gacgcgggga
     1501 aagggcgcac accgcaagcc aaattccttc ggggggcatg ccgcgtgtca gtcgcttccg
     1561 gaccggatac tgtgcaaatt gcgttccggt gcaagaaggg tagccaggcg gacacacgaa
     1621 aagatcagga aggaggggcg aaggtcgcca tgagatgctc cgccgcgcgc attccatcca
     1681 cggcggcgct gacgatgccc ccggcatacc cggcgccttc ccctgccggg aacaacccgg
     1741 cgacgttttc tgactgccca tcctctctgc gaaggatgcg aacgggggag cttgttcgtg
     1801 tttccacccc aaacgcgacg gcgtcggggg ccagccagcc gtggatagtt ttcgaaaact
     1861 catgcgacgc ttctctgacc gcctgaacga cagagggagg aagcagggac gtgaggtcca
     1921 cgagatccgc gcgagggaaa agacctttcg ccaatccgtg tacacgccgg ggaggccatg
     1981 aaccgtcgac gaaagaaacc agcggcatca ttggaagggc atacgttcct ccggtggatt
     2041 tgaatgccgc cctttcccag tgtcgctgga agtcgatgcc agacagggga tccgctgacg
     2101 cgaagtcagc ggtcgggacc tgtgcgacga tcgcgccgtt ggcgaacctg cctcctcggt
     2161 ttctgccgct cataccgttg cagaacaacc ccccgggttc ggacgcacac gggatgaccg
     2221 cacccccggg gcacacacaa aaggagaaca ccgatctccc tgtgcttgtc tgcgggcatc
     2281 ggagcacgta ttcggcaggc tgaagggatc ggtcccttcc ttcgtgggaa acggcccgtc
     2341 ggccgtactg cgccctgtca ataatctcct gccggtgttg gacgcgaatc cccattgcga
     2401 acggcttctg ttcaagggca acaccgcgtg cccgaagcgc atggtaggta tcgcgggcac
     2461 tgtgcccaca tgccaggatc agcgcgttcg tggggatgac tacgtccggc gcacaacgca
     2521 cgcccagaag cgttccccgg tcaatgagaa aatcctccac ccgggtttca aacaggatct
     2581 ccgtgcccag cgcgctcaat gtggcacgca tctccttcag cacgtagaag agcctgtcgg
     2641 ttccaacatg cggccgggct tcaaaacgaa cgctatcacc tgctccgcag cgtgcgaggc
     2701 gatcaagaac gtcatcgcgc cgccaatcct tcgatcttga cgtcagcttc ccgtcggaga
     2761 atgctccggc gccgccctca ccgaacaaga cgttcgaatc ggggtcgagt cttccatatt
     2821 tccagaagtc ggtcgcatct ctcctgcgag cctccacagg tttgccgcnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc atatttccag aagtcggtcg catctctcct
     3001 gcgagcctcc acaggtttgc cgcgctcgat cagaattggt cggtacccgt tctctgcgag
     3061 tttccaggct gcgaacaaac ccgccggccc cgcgccgacg accacgggcc tctccaaaag
     3121 tgcacgggtg ccgaattcga cacgtcgttc gggcgccgaa gccgagactt tcgcgccaga
     3181 aatccgtcgg gcctgctcca gtgtcaacgg ggcctcgact tcgatggtgt acacataccg
     3241 caggcgtgag gggtgccggg cgtcgagagc acggcgacgt acccgcaact gcccgagttc
     3301 ggcctccgga acaccgagtc tggcggcgca gagggcacgc aaggcgtctt ccgattcgcc
     3361 gaacggcacg ctgatgtcag aaaggctgag aaccagcaag ggattgaccg gggttggagg
     3421 tgagttccgc acggccggat gaagtgatgc cgacaatgtc tacggggccg tccatctgct
     3481 tctggacggc ggtgagatcc cgtgcgtccg cccagggcat gcgggcgcgg atttcgtttt
     3541 caattcccgc gacagagcct ttcgaggtgt ctgtatcgcc cttccaagcc ggatggtttt
     3601 gcagcgcccg gatgacctcg gcgtggtgcc ggcagaagcc ctcggggaac tcatcttctc
     3661 tgcccacgtc gatgaagcct ttgcgaaaca gggaccgcac cgcacgcctc acctgcttca
     3721 gggcttcctc ccaggcggcg acctgggagg gcgtcagcaa tttcttgtcg aaccattgtc
     3781 ccctgagatg atccggcagg cccgccagat cagtcgtgat atcgacgaga gggaccatgt
     3841 cttcggcgcg accagcaagg agaagaagga ttttctcctc aacggggtcc agccgccggc
     3901 gggagttttc tcgcacgccg cgatgttcgt gcccggtcat accgcacata ggctgctctt
     3961 tacggtgctg gccgcggaaa ggtgccggca cagatcaggc tgatttccgc gggtgtaaac
     4021 gcgccatacc ggattccttt gcggttccag gtgtacgtga cgtgcacaag cccgtcgctt
     4081 gactgtatta tcgcggggta cgagaactcc gcaccgccga tttccatttc ccacggtggg
     4141 gaccacgttt cccctccgtc ttcggatact gcgaggtgaa tttcgtgtct gccaccgaga
     4201 agcggggtgt ttccaggcat gtcaccggtt gccgccgaca gacgcggtga ggggtcagtg
     4261 tggttgtagg caagaacaac tctcccgtca gccagtttga cggcgtctat tccgctgttt
     4321 ggattcggga gagatgaagg ccgcgcaggc gtccacgttt cgccgctgtc aaatgaatcc
     4381 gatacgcaca cgcggccaat ttcctttcgg gatcggagca gggcgcgtat gtggcctggc
     4441 gtcacctccc agagcgtcgg ctggatgact ccccagggat tgtccggatg ggcgaggggg
     4501 ccgcgtattt tccacgcatc cgaatcggga tcgtgaaggt acacccaccc cgtccatgca
     4561 cgatagcttt ctgcggaact cgggaatgcg atcatgccgt tgtgaagcct gatgcctttg
     4621 ttcttgatcg ggccgaacag gccggccggc agaatctccg gagcggacca agtgaacccg
     4681 ccatcctcgg atctggtccg caatccactc cacttttcaa cttccttgcc ggccttgaag
     4741 tacacccaga tcgtcgttcg gtgtctgaag aagacggggt tgaaacacgg cacgcccggt
     4801 tcatcggcga ttacggaagc cggcgaccac cccgaactcg tccgtcgaga catccagatt
     4861 gcagtgtcgg gcgatccttc cggtgcgccg ccgaaccacg cgcagaggag atcacccccg
     4921 gggctttcga tgagtgtcga ggcgtgagcc tcaccgacaa tcgtttccgg gttgacgacc
     4981 atctcccatt tcatgcaatg cctttctctc tccgaaagca gagatgtatt cgacgctacg
     5041 aaatgctgac gccaagcaag cgcccgatcg cgaacgtaat ggctgccgcc gccgacccga
     5101 tcagtgccat cctgagggcg ctccagagca tgttccgccc ggtgaagaga ctcataacag
     5161 ccccaacgag gagaagcgcg gcctcggtta ccacgacagt gagggagagc gtcgcgggtg
     5221 cccgggttac gagatacggc atcagcggaa tgagcgcgcc caccgcgaag gcggcgaaag
     5281 aggcggaggc ggctgcaacc ggtgatgaca atcccgcggg atcgagcccg agttcctctc
     5341 tggccagagt atcaagcccg ttgacgggat cgttcacgat tcttgtcgcg acggcgaccg
     5401 cctgatcccg gggcaacccc ttcgtttcgt agaccatggc gagttcttcg atttcctcct
     5461 cggggaagac cgccatctcc gctttttcca gtgcgatctg gtgttcgtag agctccttct
     5521 gcgtggcaac ggagatatac tcccccgcgg ccatcgaaaa acctccggca atcagaccgg
     5581 cgactccggc aagcaggacc acttcgggct gatccgccgc tccccctgcc actccgatga
     5641 tgaggctcgc attggaaacg agcccgtcgt tcatgccgaa caccgctgcg cgcaaggcgc
     5701 cacctccctg tgcggcgcga tgccatttct cgcccgcggt ggagggggag gcgagcgcct
     5761 cctcgaccgt cgagggagtt gcacctctgg tgccgtaaat cgaaagcccg cggactttca
     5821 tccccgcgag cacgggaagc atacggcgtg gcccgagccg gaacagcaac cattgcacga
     5881 cccggacgcg gagagaaggt cggaacgcgg gcacggtggc gcccagtgaa ctgagctgtt
     5941 ccgcccatat ttccgactgg gcctcggaag ccttccggag tttggcaaac atcgccgccg
     6001 cggcccctgt ctcgacggat tccatcgctc ggtacaaata agcggacgcg cgttcttcct
     6061 cgaagctcat ccgtaccacg tcgattttct ggtcaggcat tgagacctcc tttattgccg
     6121 cgcgaaatga tcaaaacctc ccgccagttc ggaaacggca ccattctccc agcggatttc
     6181 gacgtggttc ccccggcgga atgatccgac ggtggcgacc ggtgtccggg gaaatgcgct
     6241 gcggaaacgg ccggcgatct cagtggcagc cgatgcatcc actgccgcga aaagtgcgta
     6301 atcctcaccc gcgccgagaa gatagccgtc aagttcccgg cccgtgtagc gggcaaattc
     6361 cgcgagttcg tcggaaaccg gcagggaggc gtgatcaata cgcacaccca ccccgctcgc
     6421 ctcgcagatg tgacgcgcgt ccgaagcaag gccatcggat atgtcaatca tggcgtggac
     6481 gtggttgcct gtacgattcg caagccatct cccctggtca accagcggtt cagggcgaaa
     6541 atgccgttcg agcagaatcg cctcagacgg gcagtgtgct ccacgcgtga tgtccagtcc
     6601 tgcgccggag tcccctatcc tgccaaaaag catcgccgca tcgccgggct tcgcgccact
     6661 tcgcaggagg atctccgaac gggacgcagt gccgaggagt gtgacgctga ccataaagtc
     6721 gttccttgag gacgacaggt cgcctccgat cactccgacg ccgtggcggc tcgcgagacg
     6781 agaaaacccc tggtagagtt ccgccatctc tttgcggccg tagtgttcgg gtatgcacag
     6841 cgtgacaagt gcatgccgcg ggatagcgcc catcgccgca atgtcactga gattgacggc
     6901 gagcgctttg tgtccaaggt tttcgaacga aaacccgggc ccgacgacaa aatccacccc
     6961 ttcgatgagg gcgtccgtgg tgagaacctg cacgtcatcg ccgtcagcgc gaagcactgc
     7021 gcaatcgtcg ccaacgccca cgataacgtc cgcgccgcag ggaggaaggg taccgaacag
     7081 cgttttgagc aggtccgctt ccgtcatgcc gtcggcgttc acggtatcgc ctctagtttc
     7141 agagggtgaa aaagcgttcg ccacgaacta ggtcctcgca agtctccctg tcgcggatca
     7201 cctgccaggt accgcctttc accatgactt ccgcggcgcg ccggcgggag ttgtactgcg
     7261 atgacattgc aaacccgtac gcgccggcac tcatgaccgc caggatatcc ccctgctccg
     7321 gtcgcgcgat cgagcgattc ctggccagga agtctccgga ttcgcacacc gggccgacca
     7381 cgtcggccac gaaaggctca gttccacgtt gtaccaccgg ttggatgcgg tggaatgaat
     7441 cgtagaaact cgggcggata aggtcgttca ttgcggcgtc taccaccacg aaattcttgt
     7501 cctcgtttcg cttcagatac aggaccttcg taagaagcac tgcagcgttt ccgacgatgg
     7561 accttcccgg ttccgtgatg atgccgaggc ccgcgtcgtt cagaacgtcg ccaaccgcgg
     7621 cggcccattc tttgggagtt ggaggatcct cgacgtcata ggtgatgccg aggccgccac
     7681 cgatgtcgat ccatttgagc tcgatgcctt cagcacggat atgaccaacg aggtccacga
     7741 gacgcttcgc ggcgtccacg aacggctgca cgctcatgat cggcgatccg atgtgtgcat
     7801 caatcccgat aacttcgagc cactcaagtt cggacgcccg ccggtatgtt tcgaccgccc
     7861 tgctgtgagg cacgccgaat ttcgccgttt tcagcccggt tgcagttttg ggatgcgttt
     7921 tgggatcgat ttcgggattg atgcgcaacg agattcttgc gcgacggtac tctcgctgcg
     7981 cgacacgatt cagctcgacg agttcggcct ccgactccac attgaagcag aaaatgccag
     8041 cgcggagcgc ctcgcgcatc tcctcggtgg ttttcccgac cccgctgtag acgattttgt
     8101 cgggaggaaa ccctgccagt aaggcgcggt agagctcgcc acctgaaacg atatccgcac
     8161 ccagccccat ttgcgcaaaa aggcgcaaaa tggcgatgtt ggaacatgcc ttgacggagt
     8221 agcaggtgag gtgctcacgt tctccccatg cggcatccag aacatgcgcg tgctgaagaa
     8281 gggttcggtg gctgtacaca tagcacggtg tgccgacgga ttctgcgata tcccgcaggt
     8341 cgacttcctc accgcgcaga acttcattgg cgtatgcgaa gtaatgcatg gaattgccgg
     8401 tcctctcagg cagttacggg gaagactgaa cgtacgagcg aaaagcggtg ttgaatcaga
     8461 caatgttgaa agtagacaaa gcgggtggac ggtcaagtgg aacgtttcgg attttgcgga
     8521 agaagcgcgc ggtctttgaa tgcgcggatg tcgtatcgtc agttcgccga aatggcagag
     8581 ctgcatcgcc gtcttgtcgg gcaggtggac cagcgggtag gcaaagagaa ggctatcctg
     8641 cgcaaataca tcgcggcgat tgacgtcgca cttcggaatc accccgatgc gaacgagttt
     8701 tttgcccggt ggcgcaagga atcagggatg agcgagcgcg aggcctgacc gcgtcagttt
     8761 tccagagaag actgctgctc tgcgcgatcc gcgaggctcg gagaagcaac ctcatgaatg
     8821 cggccacggt gcgcaattgg tgcgtgcaaa attcccccgc atcgtcggcc agggcgtccg
     8881 cgaagcaccg gcgacacggt gtcctgctcg aagtgcgtac gtaccgttct gacagggcga
     8941 tgtggtgagg agcatcaagt cgccttgccc gtccagatct gctacgaccg gggagtagtg
     9001 gtagaagaac gctccccgga actcccacag gagggagcca tcgcccttga gggagtacac
     9061 gtactggtcc tgttcggcga caaggacctc gtagcgtccg tcggcgttca cgtcggcaaa
     9121 cgcgagcctt cctcccacac cgagagcgaa tccggtcatc tgttccctgc tcacgaattg
     9181 ccagacaggt gtcccatcgc gttcgtacgc aacgacgaca cctccggccg tggtggcgac
     9241 tatctggcgg gtggacgaat cgcgcagaga agccatgccg acctcgatta tgggccgata
     9301 cgcctcgtgc acggcaggca caacccagcg ggaacgcact tctccgtgag catccagaac
     9361 atagatcatt cccgacgcgg ttcccgtcac gatgtgcggc tcctcgcttc gtgagaactc
     9421 cccgacagcc ggtgccacgg gagagcctcc ctccgtccgg tactgccaga gcacctcacc
     9481 gtcggcgcaa cggaacgcga tgagtccctc accgtgcgcc acgaccacga agggcccgcc
     9541 tgcgcggggg atacacacga ccggggtggt atctgcggga ggcgtaaggg aatgttgcca
     9601 gcgcactgag ccatccgtgt tgagcagcgc gacaccggcg tcatgccatg agacacacag
     9661 gcacgagggg cggccatcat cggacattgc ggcggcaatg cctccgcgca cctcgccgcc
     9721 gacgggcgta cgccacaact ccacgccgtc aggcgaaaag gcgtgaacgt ggccactggt
     9781 tgcgccgtac actatctcgt ttctgccatc ggcgttgata tccgcgatca ggggactgga
     9841 tgtgttttga acggggcggt tgcttgcctg gagctggaac acgtgccgcg acgagccgtc
     9901 ggagttcagg agttcggcgt ttgcatattg tgtgagtgcg acctcgagcc tgccgtcacc
     9961 gtcaatgtcc ccgacggtgg cgtttgactc gcgctgcggt gcctgccacc ggacgcggct
    10021 cagaccgcag atagtccaga ggccgctttg tacgaactgg caggtgatgg tgcgggcgcg
    10081 ggtgttgacg ctggcggatg tgaggatacg ccatgcgccg gatttctcat ccaggaccgc
    10141 cgggcgaacc gtgtcttcga tgagtcggtc gacatggcgg gcggagtacc cgataacgat
    10201 ctgcgcggac tcgcggtgag gggcgctcac gcggatcagc aagggagcgc gtgtccatgg
    10261 cgtcggcagg atacggtcgc cggcagattc gcgcggttct gtttctaccg tgacgctgtc
    10321 gccggggagc aatccttcca cgcggaccgg agtacgctct cccgcggcgc agccggcgaa
    10381 gtcggcaaca aggttttcga ccgtccggac agagaaggtg tgccacgcgg tgatcgttcg
    10441 tgattgcggg gaaccaccgg agaaggcttc cacggcgaag cggacctctc cttcaaacgg
    10501 tgcgaaaggc ggaatgtgga accggaaaac cgatgtctcg cgcagcggcg tacagtcggc
    10561 caacccgaca cggtacccga cggagtagtg gagcaccgct ctctgtatcc cgtcagcgtg
    10621 tcgaatctca gcctgaacaa ccgtcgggag gtggtttggc agcttggccg gcggagacag
    10681 gacgtccacc gaaggtggaa tcgtatcggg caccgcggcc gcttcccgcc tttcctgatt
    10741 tgcgttccct tcggtccgga tgagctctcc cagcgaataa agggcgaggg caagttttcc
    10801 gcttccctgg atggcatcat agtagtcacg acggcggaca ggggctgttc ctttgatgag
    10861 gccactggaa tgaacaaacg ttgtgatcgc gtattccgcg tcgcgtgtgg cgcaagagag
    10921 ccatctcggg tcaccggtgg cagcatggag ggcgcagaac gcctgaatac gttcagccag
    10981 ccgtgacgcc atgagtggag tcggaattgg ctcagcagcg agcgtgcgac catattcctc
    11041 ggcgagatcg cgccattgct ggttgcgcgt caggttgaac gcggaaatca gaacctctat
    11101 tccggctgcg acatcgcttc cgaacactgg atcgcctttt gtttccatcg tgcggctttc
    11161 ctcgtgtcga agcccgacgc atgcctcgag gtagcggcac ccgtcgtcca accattctcg
    11221 cataccggtc atcacgtaga tgtcaaacat gtctctggcg aggccgggac gggcgggata
    11281 gttgcgcggg agttgccttt tgtccgagtc cgtccagttg tcagggtaga gacctgcgcc
    11341 gtttgatttg gactggaacg ccagaagctg atttcgcgcc caacgcaggt actccgggtt
    11401 ccgcgttttt gtccacagga acgcccaggc tttggcgaat acaccggcgt acccgaggat
    11461 tgtcgcctgg tcgcgctcgg gcaggccgtc ccagaagcgc gcgtgtcggt cgtaaatcat
    11521 tgctgccttg tccacgacgt gaaaacggta aatcgcctcg atttcgcgct ggactttcgt
    11581 cggtgagatt tgccagagtt cctcccaaag tgggtcggcg tgctgcaatt cgtgccgttc
    11641 cccctcaatt cggtccgaca cgacgttgta tgcaacgtgt tcgccccagg caaagagccc
    11701 cgtttctctg ctcacacaat gttccgggaa atacgccatg catgaccagg cagcctgggc
    11761 gtaccgccga tcgcctgtgc tctcgctgag tttgataagg agccgaacgg tttcggtatc
    11821 ccagagaagg tttgactgat gcgcccacgt gaggttgctc cggtcttttc cccacatgag
    11881 gtatcctttg tcctcttccc aatcggccat cagatcgcgc cactcggcgg cagttttctg
    11941 ggcgggtacc gtcaacgtct ccagatcgat gaagtggcag aacagagggg tgtcacgggt
    12001 gccgtagtga tcggttccgt acgcgatcaa cgcatccgcg aaattctttg cgatgacaga
    12061 gaaatcgggc atcccgcacc tttcccggtg gcatccggtg agtttctggc cgctctgttc
    12121 ggatttccag taccaagaac gcaaagagaa cattggcgaa acaaactccc gcccgagcgc
    12181 acgcgccggc ttctcccttg gtgccgcgtt ccgcctgttg catactttgt tgtcggttcc
    12241 cgcaacctgg aagtcattct cgcactctgc agttttctgg ctccgcgaac cgggcttccc
    12301 cttggaggaa ttcatgtacc gccatatccc actccggcat ccgcgaccga ccgcgcagcg
    12361 cttcattgac ggattgatgg gccgtttcaa ggaaacaaaa ccgcggatgg tggagtacct
    12421 tgtggacggt tccgtcatga agcccattgc cacgtcgctt ctgggcaggg agtgggttga
    12481 cacgccaacc gatgcggaga gtcgcaggaa ggcactggat atcgcgattg agttctggta
    12541 ccgcatgggg tatgatttca tccgcattga aagaggactg gtgtttccga agcattctct
    12601 gcgcgccgat gacgtgtccg gggtgccagg aaaccagcgt acctgggcgg atgagcacca
    12661 ggggatgatc cgcacgtggg aggatttcga gaaatacccc tggccgaggc tggaagactg
    12721 tgactttttc gacctggagt atgtgagcag gaatctcccc gacggcatgg ggctgatcgc
    12781 gagccatggc ggtggaccgt tcgagcagtt gtcgcagatt ttttcgctgg aagggcttgc
    12841 gctggcgctc tacgatgacc ccgatctggt gaaggcagtg aatgacaaga tcggtaatct
    12901 catgactggc ttctaccggc acctcgtcca gctcgacaac ctcgtcgccg tgtttcccgg
    12961 agacgatatg ggattccgca caggcacgat gatcgcacct gctcatctcc gcgcgtacac
    13021 tcttccagtg catacgcgtg tcgccgaaat ggcgcacgaa cggggtctcc cgtattttct
    13081 gcactcatgt gggaatgtcc ttccgatagt cgaggacgtg atcaacgacg tgaagctcga
    13141 tggaaagcat tcattcgagg acgcgatcat tccgattcag gatttccagc gcgaatacgg
    13201 ggagaagctt gcagttctgg gtggggtgga cattgacctc ctcgcggccg gctcggttca
    13261 ggatgtgagg aaacacgtga ggtttttgat ggaaacctgt ggtgcgcgag gacgtttcgc
    13321 actggggtct gggagctcta ttcccaatta cgttccggta gcgaactacc tcgcgatgct
    13381 ggacgaagcg atagatttct cttcgtaggt atttcatggc gcgcgcgaag gctcttctgg
    13441 acaacgtttg tgtcgcactc aaccgcgagg gtgtcgcggc ggagcgacac atccc
//
LOCUS       DAOSOO010000074        13330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4672_length_13330_cov_4.696497, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000074 DAOSOO010000000
VERSION     DAOSOO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4672_length_13330_cov_4.696497"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..198)
                     /locus_tag="PLT86_07040"
     CDS             complement(25..198)
                     /locus_tag="PLT86_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36402.1"
                     /translation="MGPTGYAVMVLLVFCGILLLVMAKLAGLVFLLGGWVIKLLAVLA
                     VFIWFLPARRRPT"
     gene            complement(202..963)
                     /locus_tag="PLT86_07045"
     CDS             complement(202..963)
                     /locus_tag="PLT86_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT36403.1"
                     /translation="MGKIPVALQLYSLRDVIPYDVEGTLASVAKMGYQGVEFAGYYGK
                     SAKELRGILDDNGLLVAGTHIGLETLLGDEFDKTVEFNQELGNKFLIVPGLAEERRNS
                     VKAWKDTAKIFNDLAENVKPCGMRVGYHNHWDEFKPMEGQLPFDVFFSATVADVVMQL
                     DTGNALYGGAESVPLLKKYPGRAATVHLKECSRNEEVNFASMYGEGSVPWKEVFELCE
                     TVGKTEWYIVEQEKYLSPPLESVAEGLQALRKLGV"
     gene            complement(1048..1932)
                     /locus_tag="PLT86_07050"
     CDS             complement(1048..1932)
                     /locus_tag="PLT86_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36404.1"
                     /translation="MPCFEAGKHVTVEKPLAITLRAGKLMLDAAEKAGKVFQVAENYR
                     RSTENRAIKWAIDSGMIGKLRMIYWVDVEERLWYWTWRDHRDQAGGGWPLDGGVHFAD
                     LFRYHVGDPESLYCTVRTYNPFRYKDTDNLTGPIPVDVEDTTLAVLHFADGVTGSWTY
                     TLSGPGDKWGHRAVYGEHGSLTWGSGFKSRKTSMTMEELIAEHHRAIGPDGVEKLFPR
                     GIQDTIATELNEFVEAVLHGTPIEITGWEGYKDQAISMALYESDAAGRPVLLSDVEAL
                     RLEAYQGRLNADLEGLTQ"
     gene            complement(1857..2309)
                     /locus_tag="PLT86_07055"
     CDS             complement(1857..2309)
                     /locus_tag="PLT86_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36405.1"
                     /translation="MIDDAQDGDGEFYTRPGYGTAKVDGNTSRGGKMAERVTIGMTGC
                     GGMMGAHERGFRELWDKGIRNFEIVATCDLVKSLAEKMADQVGGWQGKRPRVYSDVET
                     MLKSEPDMMGWTLLRSTVRIIRWLCRALKPENTLQWRSRSRLHSELAS"
     gene            complement(2306..4780)
                     /locus_tag="PLT86_07060"
     CDS             complement(2306..4780)
                     /locus_tag="PLT86_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT36406.1"
                     /translation="MVDKSNPKAYTVHMIGNAHIDPIWLWRLEEGRKEVSDTCRSALD
                     RMKETPGFIFVRSSASSYVWLEEDAPDMFEEVRQRIEEGRWEIVNGWWEQPDCNIPGG
                     ESYVRHALYAKEYFVEKFGVEPTVGYNVDTFGHNGNLPQILAKSGFKSYIFFRPGEHE
                     KHLPQHFWWESPDGSRVLGIRPPHHYGFGGNVEEIKKRIVTAWEQTDDGVTEVMCFYG
                     VGNHGGGPTKLNIQGILETDAMPDAPNAKFARCDEYVEKVLAQKTDFPVVKDDLQIHA
                     VGCYTAVSAVKVLNRRAEQKLMAAERFATVANLVSHKCYPGAELRDAWRDVLFNQFHD
                     VLAGTSIHPAYEDTYAMFAKAIETADVSQRASLRAIATSVDTRGKNLALVAFNPNPWR
                     RRDLITADVEVAVVPKLVRLVADDDSEVPVQIAGVSEAGDKSTVTVMFVAELPALGFR
                     VFRLHLDEMPSFETPMVCAHPNGLSNDVLSLVVDPYSGGLVSLRAGNGPELIAAPGAN
                     FVVIKDHSDTWSHGITEFRNELGRFIANGRIRVVSQGPVRATLEVVSSFEDSTLVQHF
                     HVYPGINRVDVDVKMDFRERHRMVKLAVPTALDQITATAEIPYTYIERDWNGEELPGQ
                     KWIDVTGKVDRARFGVTMLNDGRYGFDVKESEMRMSVLRTPIYAFHDPRKVNPKETYQ
                     YPDLGTTSFRYALIPHGGDWRAIMAPRAGEEFNTPTVAFIEPHHNGALKSGAEFLEVG
                     PDNVVGMILKRTQEYANGNAADDRIIVRLYEGHGLDGIARVRLPFLGIAAEVPIGHHE
                     IKTLAFDVQNPSSAPVEVDLLERPIP"
     gene            complement(4908..5648)
                     /locus_tag="PLT86_07065"
     CDS             complement(4908..5648)
                     /locus_tag="PLT86_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT36407.1"
                     /translation="MLRGVRAVLFDYGGTLGNDEPAYAQGFTHLLTAMGYPTDMALYR
                     IASRDAEAALPPAPRDAEAYLTWRAQYRRELLRFCGVPRRELPRMEDAVAKRLRYFTT
                     LFAYAESRFVLRSLKWAGFTVGMISNISPLLPMVNKELGFEEYLDFAIASETFGCEKP
                     DRRIFEEGLRLAGVPAEKAVYVGDSPEADVAGALSVGMHPVLIARRYPLPEVAEGVTA
                     ILNLTQLFDLLGIDPWDEPSLQTPSLTR"
     gene            5823..>6702
                     /locus_tag="PLT86_07070"
     CDS             5823..>6702
                     /locus_tag="PLT86_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT36408.1"
                     /translation="MHVPQTVTYKIADRCDLKADVYVPERGDAAAVILWIHGGALIGG
                     NRKDIREEQLEFYLRNGYALVSIDYRLAPETKLPEIIADIRDAYDWLRREGRQRFGFD
                     TRRIGVAGHSAGGYLTLMTGFCVSPRPRALVSFYGYGDLTGGWYSRPDPFYCKLAPVE
                     KETAHASVGTEPVADLSLPGTLPDPRGRFQFYLYCRQQGLWPKEVSGFDPDYESKLYV
                     PFNPLWNVTPDYPPTLLLHGDLDTDVPYAQSVLMAYEMQRHGVEHELHTVRGGGHGFG
                     KPEVDTAFDRVIGFLKQ"
     assembly_gap    6703..6802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6803..8797)
                     /locus_tag="PLT86_07075"
     CDS             complement(<6803..8797)
                     /locus_tag="PLT86_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HOT36409.1"
                     /translation="MIIAENGKPRCTILLGTRAAWLERHAAEELQRYLARMANALIPV
                     EEEPVAPGPVLAIGRPGSNLFIARAIASGAVSAPPSEQSDGFVIQTVEIDDAESLVIA
                     GSSDRGTLYGVYWLLEEVLGVGFFRDGERVPNLTTVELPPLSISERPHFPERHEGNGC
                     VFTYSTSSWGVDEWKWELDWKAKRRFNTTWPFDVAHDLLPRMLADWGVDTQVPRRSEG
                     HTVHEQAFRYARSLGIRIPCIIGNLPATPELRARFPETRVLVLQYSELPPYTIVHPGD
                     PLFRRYVVEYVRRYNDSWGSDHLYIASFISESKVLEGAANLHEARLDFARAVSEALKE
                     ADPEATWIIETWSFDMDAEDVNHRWSPAQIKEYLGTVSVPHIVADLWSEERGKYLQTE
                     NFFGHPWGFGVLHSFGAGSYLHGDVSLLLKKVRDVVNQPDGKNCRMFLLQPEIIQFNS
                     FFYEFCAQVAWDPLRGNVESFVERYCRKRYGASLGEKLREPWNILVDTVYGSESGSVG
                     TITDPLYWYRPDIHLLLGWTKPDDKTATLRARRHLLIPKLRDVLELFLTQEVEVRTDP
                     LALRDFVDVARQWIAERFNIAIISARDAFLAGDEVELEKRRAECVALLDDQTRLLSNW
                     PPYRLDTKVKEHRARFGDDAERWVKHTHVWVYGDEGMHSPP"
     gene            8899..9894
                     /locus_tag="PLT86_07080"
     CDS             8899..9894
                     /locus_tag="PLT86_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT36410.1"
                     /translation="MTDSTHNRHIHQSCLEHLHLHLAVRVSPSSRPSLLRLFGTLAVG
                     TIAISTSAPFIKLAGMRPFALASGRLLGVAILYALVGRDLFSAWRGLERRERARAIVA
                     GMFLAAHLGCWIWAFEFTDLPSAVLLLVLQPLFAAWFGSFVFREKVTSSAITAVILAI
                     VGLLIITFDDLAISPKQLVGDALAAAASLFMVIFMSLGRTLRPRMSLPAYMTMTYGGA
                     GVWSLILALLMGTPLLGYPTSSYLWLLGLIFITTGVGHAAFNYVLPHVRLFTVNIATV
                     AEPALSILGAVLFLGQRLTIYELIGGALLMAALFVGVRDEKQGTAATQIEQVALE"
     gene            10029..10106
                     /locus_tag="PLT86_07085"
     tRNA            10029..10106
                     /locus_tag="PLT86_07085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Pro,seq:cgg)
     gene            10128..10406
                     /locus_tag="PLT86_07090"
     CDS             10128..10406
                     /locus_tag="PLT86_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT36411.1"
                     /translation="MSETVRTFVRVNGSVQGVGFRWYALRCAQRTGVAGWVRNMPDGS
                     VEMEVEGDPAAVNAFIEDMRIGPATADVSEVNFHRIPVKGETSFEVKR"
     gene            10403..10918
                     /locus_tag="PLT86_07095"
     CDS             10403..10918
                     /locus_tag="PLT86_07095"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOT36412.1"
                     /translation="MTIAANLIRNIPDFPKPGILFRDITPLLNNPAAFSQVIDELADA
                     TSRYRFSRIAGIESRGFIFGAPLSLKLGVGFVPIRKPGKLPARVLREEYSLEYGTNAV
                     EMHADALTKGESVLIIDDLLATGGTAGAACRLVERAGGTVAACTFVIELAALEGRKAL
                     EGREVVSLITY"
     gene            10961..11431
                     /locus_tag="PLT86_07100"
     CDS             10961..11431
                     /locus_tag="PLT86_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36413.1"
                     /translation="MIWKHAARSALLLTLFIGCNVGPDTRYFLARPLSSRGLAWEIYT
                     RGAWRVVELQGIRPPSYVKEGVLAGGNYFLFAGKFMEPNTLEFRAWTVLPPVRRVVSA
                     ESPPDTTGLPWLTGDDFPKLEDYQRFSLLAAGERWPWRGEGVEKPDSLSFLFRR"
     gene            11530..12372
                     /locus_tag="PLT86_07105"
     CDS             11530..12372
                     /locus_tag="PLT86_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOT36414.1"
                     /translation="MTPEKPANAFTVDVEDWFQVSAFRHHIGRDEWDHCQLRVEANTE
                     RLLEILASHNVRGTFFVLGWVAERVPALVQRIASAGHEVACHGYHHELVYDCYPSQFE
                     ELTHRAVGVLGELLGKPVTGYRAASFSLPRSERQWVYDILGRQGIRYDSSIFPIRRKF
                     YGHPEAPWTPFDVTTPSGTLREYPLPVVKIAGQAFPFGGGGYFRLYPYHLTASCIRRA
                     NREGRPVVFYVHPWEIDPGQPRQNVGLVTKFRHYVNIKREEERLQRLLREFKFQPLPV
                     GAAG"
     gene            12369..13043
                     /locus_tag="PLT86_07110"
     CDS             12369..13043
                     /locus_tag="PLT86_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT36415.1"
                     /translation="MNQNTTQETVTRHFDVTAEEFDSIYTGEGKSFWGKLLDRSLRKD
                     MYERFTLTVENCGDDPDRTILDVGCGTGRFSIALAQRNARVDGIDPAPTMLAIATRLA
                     SEQGVADKCRFIEADLDNFSAAEKYDYVIGIGLFDYLATPAAALKRMTGLAKKRVIVT
                     FPRRWTWRAPIRKLRLSLRGCPVFFYDRAQVEKLVRDAGGQIEEVRVVGKIYFVMFAP
                     TDSHRK"
     gene            13053..>13330
                     /locus_tag="PLT86_07115"
     CDS             13053..>13330
                     /locus_tag="PLT86_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36416.1"
                     /translation="MIRVLHVINTLAPGGAETLVTSLCQALPREGVQPLVAYLFGDGT
                     LGERLKDHGIGVERLSHGSRPSLFALPRAVSLIRSRKIDLVHAHLVYAG"
BASE COUNT         2677 a         4058 c         3659 g         2836 t
ORIGIN      
        1 gtttccctcg aagacaagac gtcgtcatgt ggggcggcgc cgtgccggca gaaaccagat
       61 aaaaaccgcc agcacggcca ggagtttgat tacccacccg ccgagaagga atacgaggcc
      121 cgccagtttt gccatcacca agagaaggat gccgcagaac acgagcagca ccatcacggc
      181 ataccctgtc ggccccatcg gttacacacc cagtttcctg agcgcctgta acccttccgc
      241 gacactttcc agtggcgggc tcaggtattt ttcctgctca acgatgtacc actcagtttt
      301 gccgaccgtt tcgcagagct caaatacctc tttccacggc acactgccct caccgtacat
      361 cgacgcgaaa ttcacctcct cgttacggga gcattccttg aggtgcacgg ttgctgcacg
      421 tcccgggtat ttcttgagga gaggcaccga ctctgcgccg ccatacaagg cgtttcccgt
      481 gtcaagctgc atgacgacat cggccacggt ggcggagaag aaaacatcga acgggagctg
      541 cccctccatg ggtttgaact cgtcccagtg attgtggtac ccaacacgca tgccacacgg
      601 tttcacgttt tccgccaggt cgttgaagat tttggccgta tccttccagg ccttcacgga
      661 attgcgccgt tcctcggcaa gtccggggac aatgagaaac ttgttcccaa gttcctggtt
      721 gaactccacc gttttgtcaa actcgtcccc gagcagcgtt tcgagaccga tgtgcgtacc
      781 ggcgaccaga agaccgttgt catccagaat tccccgcagt tcttttgcgc tctttccgta
      841 atagcccgcg aattccactc cctggtaacc cattttcgct accgaggcaa gggtgccttc
      901 gacatcgtac ggtatgacgt cacgcaatga gtaaagctgc agagcgacgg gaatctttcc
      961 catggaacac ctccttgttt tggcgcacgt gttgacaccc atctggaatg tggagagcag
     1021 ccacagaccg atgtgtggcg catacagtta ctgagtcaga ccctcaagat cggcgttgag
     1081 acgcccctga taggcttcca agcgcagcgc ttctacatcg ctcaacagaa caggccgtcc
     1141 cgctgcatcc gactcgtaca acgccattga gatcgcctgg tccttgtacc cttcccaccc
     1201 cgtgatctct atgggtgtgc cgtgcagcac cgcttcgacg aattcattga gctccgtggc
     1261 gatggtatct tgaatcccgc gcgggaagag cttttcgacc ccgtccgggc cgatggctcg
     1321 atggtgttcc gcgataagct cctccatcgt catggatgtt tttcgcgatt tgaaccccga
     1381 accccacgtc agtgacccat gttcaccgta gaccgcgcgg tgtccccatt tgtccccggg
     1441 acctgagagg gtgtacgtcc acgatccggt taccccgtca gcaaagtgca acaccgcgag
     1501 cgtggtgtcc tccacgtcaa ccgggatggg gccggtgagg ttgtcggtat ctttgtaccg
     1561 gaacgggttg taggtccgca cggtgcagta aaggctttcc gggtctccaa cgtggtaccg
     1621 gaacaggtcg gcaaagtgca cgccgccatc gagcggccac ccgcctccag cctgatcgcg
     1681 gtggtcgcgc catgtccagt accagagcct ctcctcaaca tccacccagt agatcatcct
     1741 caacttgcct atcattccag aatcaatcgc ccatttgatc gcacggtttt ccgtggaccg
     1801 gcgatagttc tccgccacct gaaagacttt tcctgccttc tcggcggcat ccagcatcag
     1861 cttgccagct ctgagtgtaa tcgcgagcgg cttctccact gtaacgtgtt ttccggcttc
     1921 aaagcacggc acaaccaccg aatgatgcgc acggtggacc gtaacaacgt ccaccccatc
     1981 atatcgggtt cggatttcaa catggtttcc acatcagaat agacacgggg ccgcttcccc
     2041 tgccagccac cgacctgatc cgccatcttt tcggccagcg atttgacaag gtcacaggta
     2101 gctacgattt cgaaattcct gattcctttg tcccagagct cacgaaatcc ccgctcgtgc
     2161 gcgcccatca tgccgccgca cccggtcatt ccgattgtca cccgttctgc catctttcca
     2221 cctctcgatg tgttaccgtc cactttcgcc gtgccgtacc cagggcgcgt gtagaactca
     2281 ccgtcaccat cctgcgcgtc gtcgatcaag gaatcggtcg ctcaagcagg tctacctcga
     2341 ccggggcaga cgaggggttc tgaacgtcaa acgcgagcgt tttgatttcg tgatgtccga
     2401 tcggtacctc ggcggcaatc ccgaggaatg gcaatcgcac cctggcaatt ccgtcaagcc
     2461 cgtgcccttc gtacaaacgg acaatgatcc tgtcgtcggc tgcgttcccg ttcgcatact
     2521 cctgcgtgcg cttcaggatc atcccgacaa cgttgtccgg accaacttcc aggaactccg
     2581 ccccgctctt caacgctccg ttatggtgcg gctcgatgaa cgccacggtc ggagtgttga
     2641 attcctcacc ggcccgcgga gccatgattg cgcgccagtc tcccccgtgg gggatgaggg
     2701 cataccggaa tgaggtcgtg ccgagatccg gatactgata cgtttccttc gggttgacct
     2761 ttcgcggatc gtgaaacgcg taaatcggcg tcctcaggac gctcatccgc atctccgatt
     2821 ccttcacgtc gaagccgtac cgcccgtcgt tcaacatggt aacgccaaaa cgcgcccgat
     2881 ccaccttccc cgttacgtct atccacttct ggcccggaag ttcttcaccg ttccaatccc
     2941 gttcgatgta cgtgtaaggg atctccgccg tcgccgtgat ctggtccaga gcagtcggga
     3001 cggccagctt gaccatacgg tgccgttcgc ggaaatccat tttgacgtct acgtcgacgc
     3061 gattgatgcc cgggtagacg tggaaatgct ggactaacgt actatcttcg aaggaactta
     3121 ccacctcgag tgtcgcccgg accggtccct gagacaccac acggattctg ccgttcgcga
     3181 taaacctccc cagctcgttc ctgaactcgg ttatcccgtg gctccaggtg tcggaatgat
     3241 cctttatcac gacgaagttc gcgccgggcg cggcaatcaa ctcgggaccg ttgcccgctc
     3301 gcaggctgac cagcccaccc gaatagggat cgacgaccag cgatagaacg tcgttggaca
     3361 agccgttggg gtgcgcgcag accatcggtg tctcgaacga gggcatctcg tcgaggtgca
     3421 acctgaacac ccggaacccc agcgcaggca gttcggcgac aaacatcacg gtaaccgtgc
     3481 ttttgtcacc tgcctccgaa acaccggcga tctgaacggg cacttcgctg tcatcgtcag
     3541 ctaccagacg taccagtttg gggactaccg caacctcgac atccgccgta ataaggtctc
     3601 tgcgccgcca cgggttagga ttgaaggcaa ccaacgccag gtttttaccg cgcgtatcaa
     3661 cgctggtggc gattgcacgc aaagaagctc tctgcgagac atccgccgtt tcgatcgcct
     3721 tcgcgaacat tgcgtacgta tcctcgtacg cgggatggat cgaggtgccg gccagcacgt
     3781 catggaactg attgaacagc acgtcacgcc atgcgtcgcg aagctctgca cccggatagc
     3841 atttgtgaga aaccagattc gccacggtgg caaagcgctc ggcagccatc agtttttgtt
     3901 ccgcgcgccg gttcaagacc ttcaccgcgc ttactgccgt gtaacacccg accgcgtgaa
     3961 tctgcagatc atctttgacg acaggaaaat ccgttttctg cgcgagaacc ttttctacat
     4021 actcatcgca ccgtgcgaac ttcgcgtttg gcgcgtccgg catcgcgtcc gtttccagaa
     4081 ttccctgaat gttcagtttg gtaggtccgc cgccatggtt tccgacgccg tagaaacaca
     4141 tcacctcggt aacgccgtcg tcggtctgct cccatgcggt aactatccgc tttttgatct
     4201 cctcgacgtt cccgccgaag ccatagtggt ggggcgggcg aattccgagg acgcgggaac
     4261 cgtcgggcga ttcccaccag aaatgctgtg gaagatgttt ttcatgctcc cccggacgga
     4321 agaatatgta gctcttgaag ccgctctttg cgagaatctg aggaaggttc ccattgtgtc
     4381 cgaacgtatc gacgttgtac cccaccgtgg gttccacgcc gaatttctcc acgaagtact
     4441 ctttcgcgta cagtgcgtgg cgaacgtacg actccccacc ggggatattg cagtcaggct
     4501 gctcccacca gccattaaca atctcccagc ggccctcctc gattctttga cgaacctcct
     4561 cgaacatgtc cggcgcgtct tcttcgagcc acacgtacga tgacgcagag cttcgaacga
     4621 agatgaatcc gggagtttcc ttcatcctgt cgagagcgga acggcaggta tcacttacct
     4681 ccttacgacc ctcttcgagc cgccacaacc atatcgggtc tatatgcgcg ttgccaatca
     4741 tgtgaactgt gtaagccttg ggattcgact tgtccaccat cggggattcc ttctgctacg
     4801 tagtcctgga tgcagtgcgc cacgcacgac ggaagatgaa gcgggtcgca cagaagcgaa
     4861 aaatcggtct ttggatcggt gtgatgatcc ggtgtcctct cgattgatca gcgggtgaga
     4921 gatggcgttt gaagcgaggg ttcgtcccag gggtcaatcc ccagaaggtc aaataactgc
     4981 gtaaggttga gaattgccgt gactccctcg gcgacctccg gaagcgggta gcgacgtgcg
     5041 atcagaacgg ggtgcatgcc cactgacagg gccccggcaa cgtcggcttc cgggctatcc
     5101 ccgacgtaaa ccgccttttc ggcaggcacg ccggcaaggc ggaggccttc ctcgaaaatg
     5161 cgccggtcgg gcttttcgca cccgaatgtt tctgatgcaa tcgcgaaatc caggtactct
     5221 tcaaaaccaa gttccttatt gaccatcggc aggaggggcg agatgttgga gatcatcccg
     5281 accgtgaatc ctgcccattt gaggcttcgc aacacaaaac gggattccgc gtacgcaaac
     5341 agcgtggtga agtaccggag tcgcttcgcc actgcatctt ccatcctcgg gagctcgcgc
     5401 ctcggaacac cacagaagcg gagaagctcc cgccggtatt gtgcgcgcca ggtgagatag
     5461 gcttccgcgt cccgcggcgc aggagggaga gccgcttccg cgtcacgact tgcgatccgg
     5521 taaagcgcca tatccgtcgg gtaccccatc gcggtcagga gatgggtgaa accctgcgcg
     5581 taggcaggct cgtcgttccc gagagttccc ccataatcga aaagaaccgc gcgcactcct
     5641 ctcaacatct tctgattgtc tccacatcta agacaggttc aaatggaaaa atcgtcgcat
     5701 tcggcatgtc ccgtttccgc cggttgcgta cgaacgcaca tgtgttttga ttccttgaag
     5761 acaggaccat ccagaagata agtgctccac ttggacgggt gcaccccgcg gaaagagacc
     5821 acatgcatgt tccccagaca gtaacgtaca agatcgccga cagatgtgac ctcaaagccg
     5881 acgtgtatgt acccgaaaga ggcgatgccg ccgcggtgat tctgtggatt cacgggggcg
     5941 cattgatcgg cggaaatagg aaagacatca gagaagagca actggagttt tatctgcgta
     6001 acgggtacgc tctcgtttcg attgactacc ggctcgcccc ggaaaccaag ctcccagaaa
     6061 tcatcgcgga tatccgcgac gcgtacgact ggcttcgccg cgagggacga cagcgcttcg
     6121 gttttgacac acggaggatc ggtgttgcgg gacattctgc aggagggtac ctcaccctta
     6181 tgacggggtt ctgcgtctca ccgcggcccc gggcgctcgt ttccttctac ggatacggcg
     6241 acctgacggg tggctggtac agtcgtccgg acccttttta ctgcaaactg gcgccagtgg
     6301 agaaagagac cgcgcacgcc tccgtcggaa ccgaaccggt agccgatctg agcctgcccg
     6361 ggacattgcc ggaccccaga ggtcgcttcc agttctacct atactgccgc cagcagggtc
     6421 tctggcccaa agaggtcagc ggcttcgatc cggactatga gtccaagctg tatgtgccgt
     6481 tcaaccctct ctggaatgtg actcctgact acccgccgac gcttttgctg cacggcgatc
     6541 tcgacacaga cgtgccgtac gcccagtccg tcctcatggc gtatgagatg cagcggcatg
     6601 gcgtcgagca cgaactgcac accgtgcgag gtggcgggca cgggttcggc aaacctgagg
     6661 tcgataccgc attcgaccgc gttatcggat tcctgaaaca gcnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nncggcggtg aatgcatgcc ctcgtctccg taaacccaga
     6841 catgtgtgtg ctttacccac cgttccgcat cgtccccgaa tcgagcgcga tgctccttca
     6901 ccttcgtgtc gagtcgatag ggcggccaat tggataacaa cctggtctgg tcgtccagca
     6961 atgccacgca ctctgccctg cgtttttcca gttcgacttc gtcgcccgca aggaacgcgt
     7021 ctcgtgcgga gatgatggct atgttgaagc gctccgcaat ccattgtcgg gcaacgtcaa
     7081 caaaatcgcg cagcgcgagc gggtcagttc tcacctccac ctcttgcgta aggaaaagct
     7141 caagcacgtc gcgaagtttc gggatcaaca ggtgcctccg tgcccgcaac gttgccgtct
     7201 tgtcatcggg tttggtccat ccgaggagaa ggtggatatc cgggcggtac cagtacagcg
     7261 gatccgtaat cgtgccgaca cttccgcttt cggacccgta aaccgtatcc acgaggatgt
     7321 tccagggttc acgcaacttt tcgccgagag atgcaccgta gcgtttccgg caataccgtt
     7381 ccacgaagct ttcaacattg ccccggagag gatcccacgc aacctgcgcg cagaattcat
     7441 agaaaaagct gttgaactga atgatttccg gctgaaggag aaacatccgg cagttcttgc
     7501 cgtccggttg gttgaccacg tcccggacct ttttcagcag aagactcaca tccccgtgca
     7561 gatagctgcc tgcaccaaaa ctgtggagaa cgccaaagcc ccaggggtgg ccgaaaaagt
     7621 tctccgtttg caggtacttg ccacgctctt ccgaccagag atcagccacg atgtgcggaa
     7681 ctgagacggt cccgaggtat tccttgattt gcgccggaga ccatcgatgg ttcacgtctt
     7741 ccgcgtccat atcgaaagac cacgtctcga tgatccaggt ggcttccgga tcggcttcct
     7801 taagggcttc ggagactgca cgggcgaaat ccagccgcgc ttcgtgaagg ttcgccgcac
     7861 cttccagaac cttactttcg ctgatgaagc tggcgatgta aaggtggtcg gagccccacg
     7921 agtcgttgta tcgccgcaca tactccacaa cgtagcgacg gaaaagcgga tctcccgggt
     7981 gaacgatcgt ataaggggga agctcactgt actggagaac gagcacgcgt gtttcgggaa
     8041 accgcgcacg taactcggga gtagcgggaa ggttcccgat tatgcaggga attcttatcc
     8101 ccaggctccg cgcgtagcga aatgcttgtt catgaactgt gtgcccctcc gagcgtcgag
     8161 gtacctgtgt gtctacaccc caatcggcca acattcgcgg aagcaggtca tgagcaacat
     8221 cgaacggcca cgtagtgttg aatcgcctct tcgctttcca gtccaactcc cacttccatt
     8281 cgtccacgcc ccaagaagac gtagaatacg tgaacacaca gccgtttccc tcatggcgtt
     8341 ccgggaaatg aggtcgttcc gagatcgaaa gtggtggcag ttccaccgtg gtcagattcg
     8401 gcactcgttc cccgtcccgg aaaaacccca cgccaagaac ctcttccaga agccagtaca
     8461 ccccgtaaag ggtgcctcga tcgctgctgc ccgcaatcac gagcgattcc gcatcgtcaa
     8521 tttccactgt ctggatcacg aagccatcgg actgctcaga tggcggcgcg ctcaccgcac
     8581 cggaagcgat cgcccgggcg ataaacagat tgctcccggg gcggccgata gccagcacag
     8641 gacccggcgc caccggttct tcttccaccg gaatcagagc gttcgccatg cgcgccaggt
     8701 atcgctgcaa ctcctcggca gcatggcgct cgagccaagc agctcgcgtt cccagaagaa
     8761 ttgtgcaacg tggtttcccg ttttcagcaa tgatcatttc tacctccggt gcaaagcgtg
     8821 gggccgcagc ccgtttcgtt tgagagtata cgggccgcgc ccgcacgttc ccacacgagg
     8881 gagtgtcaaa ggcgggcgtt gaccgactcc acgcacaacc ggcatattca tcaatcctgt
     8941 ctcgaacacc tccacctcca tctggcggtc cgcgtgtctc catcttcgcg tccttcactc
     9001 ctgaggttgt ttggtaccct ggcggtgggc accatcgcaa tcagcacatc cgcgcctttt
     9061 atcaaactgg ctggaatgcg tcccttcgcg ctggcgtccg gaaggcttct cggcgtcgcg
     9121 attctgtacg cgctggtcgg cagagacctt ttcagcgcgt ggcgtggact tgagcgccgt
     9181 gaacgcgcac gagccattgt cgccggcatg ttcctcgccg cccatctggg gtgctggatc
     9241 tgggcattcg aattcacgga ccttccgagc gcggttctgc tgctggtgct ccaacccctt
     9301 ttcgccgcgt ggttcgggtc gtttgtgttc cgggaaaaag ttacctcctc cgcgattacc
     9361 gccgtcatac tggcaatcgt ggggcttctc atcatcacgt ttgatgacct cgcgatttcc
     9421 ccgaagcaac tcgtaggaga tgcccttgca gcggcagcct cgcttttcat ggtcatcttc
     9481 atgtcgctcg gcagaacact ccgcccgcgt atgagccttc ccgcgtacat gacgatgacc
     9541 tacggcggag cgggggtgtg gtccctgatt ctggctcttc tcatgggtac tcccctcctc
     9601 ggctacccga cgtcgtcgta cctctggctg ctcgggctga ttttcatcac aaccggagtg
     9661 ggacatgcag cgttcaacta cgtgttaccc catgtgcgcc tgttcacggt gaacatcgcc
     9721 acggtcgccg aaccggcgct ttccattctc ggagcggtgc tgtttctcgg gcagcgtctg
     9781 acgatctacg aactgatcgg tggcgctctg ctgatggccg cgttgttcgt cggtgttcgt
     9841 gacgaaaaac agggaaccgc ggcgacgcag atcgaacagg ttgctctcga atgaccggag
     9901 gtgcgcatcc gcgtaacggc cgctgcccgt ggaagggcaa tctccgcgct gaaagaggtt
     9961 tgcggccttg ccgttttcgg gcgcagccgg tatcttttgt aatcttcaga atcgaaccgt
    10021 tgtaaagtcg gggcgtagcg cagtccgggt agcgcacctg cttcgggagc agggggtcgg
    10081 tggttcaaat ccactcgccc cgaccaactt cctttccttg ccctttcatg tccgaaacag
    10141 tacgcacgtt cgtccgcgtc aacggcagcg ttcagggagt cggctttcgt tggtacgcgc
    10201 tccgctgtgc gcagcgcact ggcgtcgccg gctgggtcag gaacatgccg gacgggagcg
    10261 tcgagatgga agtggaaggg gaccctgccg ccgtgaacgc gttcattgag gacatgcgca
    10321 tcggaccggc caccgccgac gtgtcggagg tcaatttcca ccgaataccc gtgaagggcg
    10381 aaacgagctt tgaggtgaaa cgatgactat cgcggcgaac ctgatcagga acatccctga
    10441 tttccccaaa cccggcatcc ttttccgcga catcacccct ttgttgaaca accccgctgc
    10501 gttctcgcag gtaattgacg aacttgcgga cgccacctcc cggtaccgtt tctcgcgtat
    10561 cgcaggtatt gagtcccgtg ggttcatttt tggcgcccct ctttcgctga aactgggggt
    10621 ggggttcgtg cccatcagaa aaccgggcaa gctgcccgcc agagtgctgc gtgaggaata
    10681 cagcctcgag tacggaacca acgcggtaga aatgcatgct gacgcgctta cgaaaggcga
    10741 atcagtcctc atcatcgacg atcttctcgc cacgggggga actgccggcg cggcgtgccg
    10801 attggttgag cgtgcgggcg gtaccgtggc ggcgtgtacg tttgtgatag aactggcagc
    10861 cctcgaaggc agaaaagcac tcgaaggaag ggaagtcgtc tcgctgatca cgtattaatc
    10921 atcgactgat tcatttctcc ccagtgaggt cgcggggcac atgatctgga aacacgctgc
    10981 acggtcggct ctcctcctca cgttgtttat tggctgcaat gtcggcccgg acacccggta
    11041 tttcctcgca agaccgttat cctccagggg gttggcgtgg gagatctaca cgcggggcgc
    11101 atggcgagtg gttgagttgc aggggattcg tccgccttcc tacgtcaagg agggtgtgct
    11161 cgccgggggc aactatttcc tttttgccgg aaagttcatg gaacccaaca cactcgaatt
    11221 ccgtgcatgg accgttctgc ctcctgtccg ccgcgtggtt tccgccgaat cccctcctga
    11281 cacaaccgga ctgccctggc tgaccgggga cgatttccca aaacttgagg actatcagcg
    11341 gttctcgctt ctcgccgccg gcgagcggtg gccgtggcga ggagaaggcg tcgagaaacc
    11401 cgattccctc tcgtttctgt ttcgccgtta ggcgagtatg tttgcttcag gtgaaaaggc
    11461 gggccgtggc gagatgatcg gaacgccttt ttcggaccga caaccagaaa cacaagggat
    11521 attcccggcg tgacccccga aaaaccggca aatgccttca ccgtcgatgt ggaagactgg
    11581 tttcaggttt ccgcgttccg gcaccatatc ggaagggacg aatgggacca ctgccagttg
    11641 cgagttgagg ccaatactga gcggctgctg gaaattctcg cgagccacaa cgtacggggg
    11701 acgttctttg tgctcgggtg ggttgcggag cgcgtgccgg ctctcgtcca gaggattgcc
    11761 agcgcaggac acgaagttgc ctgccatggg taccaccacg aactcgtgta cgattgttat
    11821 ccctcccagt tcgaggaact cacgcacaga gccgtcggag tgctcggcga attgctggga
    11881 aaaccggtga ccggctacag ggccgcgagt ttctcccttc cccgttcaga gcggcaatgg
    11941 gtgtatgaca tactcgggcg tcaaggcatt cggtacgatt ccagcatttt cccgatccgt
    12001 cggaaattct acggccatcc tgaagccccc tggacccctt tcgatgtgac aacgccgagc
    12061 gggacactgc gcgaataccc cttgcccgtc gtgaagatcg ccggccaggc attccccttc
    12121 gggggtggag ggtacttccg gttgtatccc tatcatctca cagcctcttg tatacgcaga
    12181 gccaatcgtg aaggccggcc ggtcgtcttc tatgtgcatc cgtgggaaat cgacccgggt
    12241 caaccacgcc agaacgtcgg tctggtaacg aaattccgtc attacgtaaa catcaagcgt
    12301 gaagaagaac ggctgcagag attgctgcgc gagttcaagt ttcaaccact tccagtcgga
    12361 gccgcgggat gaatcagaac actacccagg aaaccgtgac cagacacttt gacgttactg
    12421 ccgaggaatt cgattcgatc tacacaggcg aaggtaaaag cttctgggga aagcttttag
    12481 accggtcgct cagaaaagac atgtatgagc ggttcacgct caccgtcgaa aactgcggcg
    12541 atgatcctga ccgaaccatc ctcgatgtcg gatgcggaac cgggcgcttt tccattgcgc
    12601 tcgcgcaaag aaatgcgcgc gtggatggga tagaccctgc gccgaccatg ttggccatcg
    12661 ccaccagact cgccagcgag cagggcgtgg cagacaaatg ccgcttcatc gaagccgacc
    12721 tcgacaattt cagcgccgcg gagaaatacg attacgtcat cggaattggc ctgttcgact
    12781 acctcgcaac ccctgccgca gctctcaaac gcatgaccgg ccttgcaaaa aaacgcgtga
    12841 tagtgacatt ccctcgccgg tggacatggc gtgcgccaat tcgcaaactg cggctgtccc
    12901 tcagaggctg tccggtgttt ttctacgatc gcgcgcaagt cgagaagctt gtccgggacg
    12961 cgggagggca aattgaggaa gtccgtgtgg tggggaagat ttacttcgtc atgttcgcgc
    13021 cgaccgactc tcatcgcaaa tgaccgtccg cgatgatccg tgtcctccac gtcatcaaca
    13081 ccctcgctcc gggcggggcg gaaactctcg tcacgagcct gtgccaggcc ctcccgcgtg
    13141 aaggtgtcca gcctctggtg gcctatctct tcggggacgg cacactcgga gaacgcctga
    13201 aggaccatgg catcggggtg gaacgcctca gccacggtag ccgaccctcc ctcttcgcgc
    13261 ttccccgcgc ggtcagcctg attcgatccc ggaagatcga cctcgtacac gcccatcttg
    13321 tctacgccgg
//
LOCUS       DAOSOO010000075        12851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4863_length_12851_cov_5.892623, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000075 DAOSOO010000000
VERSION     DAOSOO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4863_length_12851_cov_5.892623"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLT86_07120"
     CDS             complement(<1..941)
                     /locus_tag="PLT86_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36417.1"
                     /translation="MEQDGIHAMVPRERMTESAKKLRDTYARKPGIPLFRREFGYFTM
                     ERWKREGMPQDIPLETLFHYDPPGSHGLGQLGWCEAAFLPAFEDKVIERRGEYEVVQD
                     YAGRHVLFFTGRRNGFMPTYLTHPVRDQKTWEENVKWRLDPSTPERYKTLPERMAKAR
                     EAAGKGLMIVQNIIGAYMYLRSLIGPEDLLIAFVDQPELIHDCMRAWFELADAVTARH
                     QEYVTIDELFFGEDICYNHGPLISPAMMREFLLPYYQQLVTNTKRRQIDKTRHLYLQI
                     DTDGFAPPVIPLYKEIGMDVMSPFEVAAGCDLLKIAR"
     gene            complement(958..1986)
                     /locus_tag="PLT86_07125"
     CDS             complement(958..1986)
                     /locus_tag="PLT86_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36418.1"
                     /translation="MLPRERALRAIHFDHPDMMPVECYAAPTGLHEHGERLAELLSRY
                     PTDFGDTTHVSVPHPPPEDFDAEGRYHAFHTDEWGVVWEHNIFGAWGHPYKRPLDDIA
                     NLARYKAPDPPPMSGPDFDAARAAAARHKQMYYLRHGGGMLIEMMRSVRRFEDVLMDV
                     QEDTPEINGLADLILEYDEKLIAHALALDVDGISIGDDWGTQEALMVSLPVWRRFFKP
                     RYDRLFAPVRAAGKDIHFHSCGQVTDILPDLAELGVNSIWPQLALYDLPTLAHTCRDL
                     GLAIALHIDRSHLMTFGTPEEVRRNVAEVASIFRKPDGGAWWYIEIDNGFPWENVTAL
                     FEAIYAHR"
     gene            complement(2094..3281)
                     /locus_tag="PLT86_07130"
     CDS             complement(2094..3281)
                     /locus_tag="PLT86_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36419.1"
                     /translation="MQESLHPLDPSPFGVVAMLRTRPEELEGAARLAVEAGVRWTRED
                     ILWHQVQPERTAWRWERYDRELGVLKARGINVLGLLCYGTPWAMSMRDANGNPDIWSL
                     PDLSAWSDYVSAVVERYRGQIHAWEIWNEPNSQTFWHPRPDPREYARLLIAGAEAVKR
                     ADPTAWVLGCNTGDVDPWFHRAVFDEGGWGHLDIIGVHPYRPPHTPERTDMVGDLLQL
                     ASLSAERGRVKPLWITEIGIASFPGVDGASEWWQAVHVLRYYLLALASGLVEKVFWYD
                     FRDDGSDPKEEQQNFGLLRRDWSPKPSYEAFRLMAQTLEGFEPNGRFELGADVVCLRF
                     RRGAEERYAVWSSGKNMRRPVPAPAGRVTLAKFPESPREIEAVGGWVTVDVNACPLFL
                     VPA"
     gene            complement(3292..3762)
                     /locus_tag="PLT86_07135"
     CDS             complement(3292..3762)
                     /locus_tag="PLT86_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36420.1"
                     /translation="MSPILPCVLLLCMLPPANAQTKPADPDTAGIQIKLPPDSYYERG
                     RLAGIRLYQTRKAAAGGYAAGLFGPPGWGVGWIALKAMKPAHVPSAQIAELDSSQMAD
                     FTRGYTDYVREARNAAFVSGAVKGTTTTAVVLVVGGLVMSVFLFVILSALTASG"
     gene            complement(3788..5158)
                     /locus_tag="PLT86_07140"
     CDS             complement(3788..5158)
                     /locus_tag="PLT86_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36421.1"
                     /translation="MPEYALKPCSIPLIDTYDVIGAGGGPAGCAAATAAAREGARTLL
                     LESDSALGGMGTLGLVPWFCGYHDREKIIARGLAMHVHDRLRENMPHFAEWLAKNPLH
                     DPPIDPELLKRIYDDMVTGAGADVLFMSRMTAVEKSADDEVEALIVASKAGLRAYRAK
                     VYVDCTGDGDLAAWAGAPFEKGMEDGAMQPGTHCFVIANIDEDALAKGPRIHFYDPGS
                     PIHEAIRSPKYPDIIDPHTCNMKIGPGVYGFNTGHVYGVDNTSPESISKALLHGRKMT
                     AQYRQAFADLHPAFRNAFLAATGSQMGIRETRRIIGDYKITVDDYLARRSFPDEICRN
                     AYGIDVHGQKNRIGALTNAGIDAFKKANEELTRPYEKGESFGVPYRCLTPKGLKNVLV
                     GGRCISADRQANGSIRIMACCLNTGEAAGIGAAMAAKDTRDVHGVNTDELRAKLKAFG
                     AYLPTA"
     gene            5416..8985
                     /locus_tag="PLT86_07145"
     CDS             5416..8985
                     /locus_tag="PLT86_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT36422.1"
                     /translation="MFRSTPSARRWCVLTAALVLSSVPFLRAGTTGKITGTVRQPGGQ
                     PIPGVAVQISGLRLGSITDAEGRYFILQVPPGTHQVEASMVGFRTAVTQNVSVSADRT
                     TMLNFTLQEETIEVEALVVTAGRPPIEADVTSSQTTVNARRVAEIPVNQMLDVLAYEP
                     GVSLSRDTELNIRGGGATQVRFQVDGLDRTDGLTNKPYTQLNQTLVAEVTLLTGGFNA
                     EYGNVRSGMVNVVTKDGTERGVGKGWVSGVFSGTPTARKKHFGPGAYDEAQFDYYTVM
                     RSDSSTRGPTGAADPYGAIYWPNLYESTRNDTAFRRVWTAAPNMYKVFDGWNARVTTA
                     NANNRGAGVYGKGTRTKPWTAAQMIEAWRYETNLNENVWGYGNKPDWSLDIAAGWGLP
                     HKLGGIVVGYVTNKEMTVVPALRPYYVDQTFDVKLTLTPIDRLKWSFSYMYGTSESTG
                     NGTAGYDPEMASTAGSVAIGSDPVSFRTASDLLGTVNQSKTAAGNNRLNLSYNSLLDG
                     SFWQWGTALTYTLSPHTFATAAFGQTRSKWDLQRDMPRADINDFSPTGSYRPPSSFSY
                     GAWLLMLIPLSDWSDINGDGTPDRPTSAEDATAPGRAYMGNPYGYGAAYPFVPSRTVF
                     VNRQMVFTPGDTAVVVSPQGWAQDGYFDLSGTFWLGEGGTQWMTSNSVQTAVRGDVTH
                     VIGDHTIKAGAEFLLSDLEYHTEQDARLLGNRFSVNYRDYGGKYPAARPNILGVFLQD
                     KFESQGLIMNYGLRLERFDGGAPYYMPDLIYDDRLYSPLHGKYWFDSLLVARGWNVND
                     PAWGPLPGNQYEAWTKFVKNGVPEQFPMPWDVMNVLPQRDAKAHWRLAPRFGISHPVS
                     DRTKFFFNYGIFYSMAKPAHMYGTGLHDARIGGGGGRFEHMFNPELRPARTTSYEVGF
                     EHLFPLNFVLKTTGYTKANVDQVTGLQVRTGSGDGYRIYRNANYQDIRGLEIKLSRNG
                     RFVSGWATYQIIATRSGQTGLEELRQNIAASSYYTPLVSTNSPADVVMASIRVGTPLE
                     WGLLRGGWGASILQSYSESTGEILYNPNAVPRRELPPEYVLRGRDSWGTSAKLTKDIS
                     LPRQRHVSLYLDINNLFNTKYLNGTYMNGNDYLAYIVERRAQGETDLRVGDPSTWDAL
                     TQPYRVKKADGTYTAWKAPISPRTDWLMFLYPRSYRAGIRFDL"
     gene            9122..10459
                     /locus_tag="PLT86_07150"
     CDS             9122..10459
                     /locus_tag="PLT86_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOT36423.1"
                     /translation="MYNEPLRPQFHFTARAGWLNDPNGLVFFDGVYHMFFQHNPFDTK
                     WGNMTWGHAVSTDLVHWRQVENAILPDRLGTAFSGSAAVDWRTTTGFGEAGVPPLVAM
                     FTSAGGTSPESAGQPFTQSIAFSADGGTTWQPYSGNPVLGHIVESNRDPRILWHEPTE
                     SWVMALYLDKNDYALFTSSNLKKWTRTCDVRIPETSECPDLFPMALDDDPAHVKWVFW
                     GANGCYLLGAFDGNTFTPEEGPYRLDSGANFYAAQTWSDIPEADGRRIQIAWMNGGQY
                     PGMPFNQQMSFPCALSLRTTSAGVRLCQMPVREIECLREDSVIRTDVALLPGRLLDAS
                     AGELLDIEAEIEPWRAKAIEWSIRGNAIRYDAEKHLLSCCGREAPVDLSGGLLRLRIL
                     LDRTSLELFSGDGNVGFHQCFLPDAANTGVELAATGGEARIRSLAVHRLRSAW"
     gene            10941..>12851
                     /locus_tag="PLT86_07155"
     CDS             10941..>12851
                     /locus_tag="PLT86_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36424.1"
                     /translation="MTSATNTAQVTRSINCFRIGTPQWLSDERFAALLALFDRYPGVT
                     DEVTFFTSETHPPLPLEVLRDRCVLLSRRMDEVRRHGYQTGINILATIGHHEENLPNS
                     LDGPYTRMTDPDGRISRGVLCPNDEQAREYIREAYRLITEANPDYIWVDDDVRLFGHG
                     PILAGCFCDTCLAAFSRETRREWSRESLREAVNSGPLADRLMYRRAWLAHNRATIARL
                     FSLIEQTVHAIRPGLPIGFMTGERYFEGYDFDAWAEVLAGPSAPRGAPVRWRPGGGTY
                     TDEKLDNIVGKAHDIGRQVAFLPSAVTVIQSEIENFPYQRLKKSAKATALEAAAYIAA
                     GCTGAAYNVLSMYDEPLDEYEPLVRVLTENRPFLDALAKAGGRSSPVGLHSGWSKDSF
                     ATLQLAEGDWLFPREGVPGGQTNEFHEIGIPPAYSLDAAQAVALTGNGVRAFDRRTLE
                     RILSGGVYLDAEAARALEEMGHAELVGFRVGEAFPIDAIEQFLPNPLCGAFAGRERDG
                     RQSFWKRTAYALEPMPGAEPLARLVDYGRKELAACGLGVFENSLGGRVCVAGYAPWTF
                     LQNLSTSSRTKAIVRWLSRETLPAYVESFHRIVLWVREPEPGKVAIALLNASLDPARQ
                     ASLLVRTAANELR"
BASE COUNT         2308 a         4220 c         3970 g         2353 t
ORIGIN      
        1 tcccgggcga tcttcagcag gtcgcacccg gcggccacct cgaacgggct catcacgtcc
       61 atgccgatct ctttgtagag cggaatcacc ggcggcgcga acccgtccgt gtcaatttgg
      121 aggtagaggt ggcgtgtttt gtcgatctgc cggcgcttgg tgtttgtcac gagctgctga
      181 taatagggca ggaggaactc gcgcatcatg gccggggaga tgagcgggcc gtggttgtag
      241 cagatgtcct cgccgaagaa cagttcgtcg attgtcacgt attcctggtg ccgcgccgtc
      301 acggcgtccg ccaactcgaa ccacgcacgc atgcagtcgt ggatgagttc gggttgatcc
      361 acaaatgcga tgagcaggtc ttccgggccg ataaggctgc ggaggtacat gtaggcgccg
      421 atgatgttct gcacgatcat cagtcctttg ccggcggcct cccgcgcttt tgccatgcgt
      481 tccggcagcg ttttgtagcg ttccggagtt gaggggtcga gccgccactt tacgttctct
      541 tcccacgtct tctggtcgcg caccggatgg gtgaggtagg tcggcatgaa cccgttgcgg
      601 cggccggtga agaagagcac gtggcggccg gcgtagtcct gcaccacctc gtattccccg
      661 cgtcgttcaa tgactttgtc ctcaaacgcg ggcagaaatg ccgcttcgca ccagccaagc
      721 tggccgagac cgtggctgcc ggggggatcg tagtgaaaca gcgtttccag cgggatatcc
      781 tgcggcatgc cctcgcgctt ccaccgttcc atggtgaagt aaccgaactc ccggcggaag
      841 agcggaatgc cgggcttcct ggcatacgtg tcgcgcagct ttttggccga ttccgtcatc
      901 cgctcgcgcg gcaccatggc gtgtatgcca tcctgctcca ttgtgcggtt ctcctgccta
      961 gcggtgcgcg tagattgctt caaacagggc ggtaacgttc tcccacggga agccgttgtc
     1021 aatctcgatg taccaccacg cgccaccgtc gggtttgcgg aatatggaag ccacctcggc
     1081 gacgttccgg cgcacctcct ccggcgtgcc gaacgtcatc aggtgcgagc gatcaatgtg
     1141 cagggcgatc gccagtccga ggtcccggca tgtgtgcgcg agggtaggca ggtcgtagag
     1201 cgcaagctgc ggccagatgg agttcacacc cagttcggca aggtcgggca ggatgtccgt
     1261 cacctgaccg cacgagtgaa aatgaatatc cttgccggcc gcgcggacgg gcgcaaacag
     1321 gcggtcgtac cgtggcttga agaaccgccg ccagaccggc aggctcacca tcagggcttc
     1381 ctgcgtgccc cagtcgtcgc cgatgctgat gccatccacg tcgagcgcaa gcgcatgggc
     1441 gatgagcttc tcgtcgtact cgaggatgag gtcggcgagc ccgttgatct cgggcgtatc
     1501 ctcctgcacg tccatcagga cgtcctcgaa ccggcgcacg gagcgcatca tctcgatcag
     1561 catgccgccg ccgtggcgca ggtaatacat ctgtttgtgc cgggccgccg ccgcgcgggc
     1621 cgcgtcgaaa tcgggtcccg acatgggtgg cgggtcgggt gctttgtacc tcgcgaggtt
     1681 ggcgatgtcg tccagcggcc gcttatacgg atgcccccac gcgccgaaga tgttgtgttc
     1741 ccacacgaca ccccattcgt ccgtgtggaa cgcatggtag cgaccctcag catcgaagtc
     1801 ctcgggcggc ggatggggca cgcttacgtg cgtcgtatcg ccgaaatcgg tggggtagcg
     1861 ggagagcagc tccgccagcc tctccccgtg ctcatgcaga cctgtcgggg cggcgtagca
     1921 ctccaccggc atcatgtcgg gatgatcaaa gtggatggca cggagcgcgc gttcacgggg
     1981 gagcatacgt ttgccttcta attcggtggt aaagccaagt tgtttcgcgg ttcagaaatt
     2041 agcacggcgc aggcggggaa ccacggggga cctttgcgct gctgacggca tggctatgcc
     2101 ggcacaagaa aaagcggaca ggcattcacg tccaccgtca cccatccgcc aaccgcctca
     2161 atctccctcg gcgactcggg gaatttggcc agcgttaccc gccccgcagg cgcgggcacc
     2221 ggccggcgca tgtttttccc gctcgaccag acggcgtatc gctcctcggc gccgcgccgg
     2281 aaccgcaggc acaccacgtc ggcgcccagc tcaaacctcc cgttcggttc gaagccctcc
     2341 agcgtctgcg ccatgagcct gaacgcctca tacgacggct tcggagacca gtcgcgcctc
     2401 aggagcccga agttctgctg ttcctctttc gggtcgctgc cgtcgtcgcg gaaatcgtac
     2461 cagaacacct tttccacaag cccgcttgca agcgccagca gataatagcg caggacatgc
     2521 accgcctgcc accactcgct tgcgccatcc accccgggga acgacgcgat gccgatttcg
     2581 gtgatccaga gcggcttcac ccggcctcgc tcggcgctga gcgaggccag ttgcagcagg
     2641 tcgccgacca tgtcggtccg ttcgggcgtg tgcggcggcc ggtacggatg cacaccaatg
     2701 atatccagat gaccccaccc gccctcgtcg aacaccgcgc ggtggaacca gggatccaca
     2761 tcgcccgtgt tgcacccgag cacccacgcg gtcgggtcgg cgcgcttgac cgcctccgct
     2821 ccggcgatga ggagccgggc atactccctc ggatcgggcc gcggatgcca gaacgtctgc
     2881 gagttcggtt cgttccagat ctcccatgcg tgaatctgcc ctcggtaccg ctccaccacc
     2941 gcgctcacgt agtccgacca cgccgagagg tcgggcaggc tccagatatc cgggtttccg
     3001 tttgcatcgc gcatgctcat cgcccacggg gtgccgtaac acaaaagccc cagcacgttg
     3061 atgccgcgcg ccttcaggac gcctagctcg cggtcgtagc gctcccaccg ccacgcggtg
     3121 cgctccggct gaacctggtg ccagagaatg tcctcccgcg tccagcggac gcccgcctcc
     3181 acggcaagcc gggccgcgcc ttccagttcc tcggggcgcg tgcgcaacat ggcgaccacg
     3241 ccaaacggcg acggatcaag gggatgaagt gactcttgca tgggcaaata cctaccctga
     3301 cgcggtcagc gccgagagaa tgacgaacag aaacacgctc atcaccagcc ctccgacgac
     3361 caggacgacc gcagtggtgg tggttccctt cactgcgccc gagacgaagg cggcgttccg
     3421 cgcctctctc acgtagtcag tgtaccctct ggtgaaatcc gccatctggg aggagtcaag
     3481 ctccgcgatc tgcgcggaag gaacgtgagc cggtttcatg gccttcaggg ctatccagcc
     3541 gactccccac ccgggcggcc cgaacagccc cgcagcgtat ccgccggccg ccgcctttcg
     3601 tgtctggtag agccgtattc cggccagccg cccgcgttcg tagtagctgt cgggcggcag
     3661 tttgatctgg attcccgcgg tatcggggtc cgccggcttc gtttgcgcgt tcgcaggggg
     3721 aagcatgcac aacagaagca cgcatgggag aatcggactc atccttcacc tcccgcaaca
     3781 gatcccgtca cgccgtgggc agatacgccc cgaacgcctt gagtttcgcc cgcaattcgt
     3841 cggtgttgac gccgtgcacg tcgcgcgtgt ctttcgccgc catcgccgcg ccgatgcccg
     3901 ccgcttcgcc ggtgttgagg cagcacgcca tgatgcggat gctgccattc gcctgccggt
     3961 cggcggagat gcagcgcccg cccaccagca cgttcttcaa gcctttcggt gtgaggcagc
     4021 ggtacggcac cccgaagctc tcgcccttct cgtacggccg cgtcagctcc tcattcgcct
     4081 tcttgaacgc atcgatcccc gcattcgtca gcgcgccgat tctgttcttc tgcccgtgca
     4141 cgtcaatgcc gtaggcgttc cggcagattt catccggaaa actgcgccgg gcgaggtagt
     4201 cgtccacggt gatcttgtaa tcacctatga tacggcgcgt ctcgcggatg cccatctggc
     4261 tcccggtggc ggcgagaaag gcattcctga acgcggggtg caggtcggcg aacgcctgcc
     4321 ggtactgcgc cgtcattttg cggccatgaa gcagcgcctt cgagatgctt tccgggcttg
     4381 tgttgtccac gccgtacacg tgccccgtgt tgaaaccgta cacgccgggg ccgatcttca
     4441 tgttgcacgt gtgcgggtcg atgatgtcgg ggtacttcgg cgatcggatc gcctcatgaa
     4501 tggggctccc cgggtcgtag aagtgtatcc gcgggccctt tgccagtgca tcttcgtcaa
     4561 tgttggcgat tacaaagcag tgcgtgcccg gctgcatggc gccgtcttcc atgccctttt
     4621 cgaacggcgc gcccgcccac gcggcaaggt cgccgtcgcc cgtgcaatcc acgtacacct
     4681 tcgcccggta cgcccggagc cctgccttgc tcgcaacgat gagcgcctcc acctcgtcgt
     4741 ccgccgactt ttccaccgcc gtcatgcgcg acatgaacaa cacgtccgca ccggcgccgg
     4801 ttaccatgtc gtcgtaaatt cgtttcagca gttccggatc aatgggcggg tcgtgcagcg
     4861 ggttctttgc cagccactcc gcgaaatggg gcatgttctc gcgcaggcgg tcgtgcacgt
     4921 gcatcgccag cccgcgggcg attattttct cccggtcgtg gtagccgcag aaccagggca
     4981 ccaaccccag cgtgcccatc ccgccgagcg cgctgtcgct ttccaagagg agcgtgcgcg
     5041 cgccctctcg tgccgcggcg gttgcggcgg cacaaccggc ggggccgcca ccggcaccga
     5101 tgacatcgta cgtgtcgatt agtggaatcg agcacggttt cagcgcgtat tcgggcatgt
     5161 cagggtccct ttctcccgcg ggtgaggcgg gatgtttgcg ggtgatcgtg atgactataa
     5221 catagccgcg tgggtgcatg gtgggaactg cgctttgtac ttgcgcgacg gtgcgcgcac
     5281 aacgcgggca gccgagttga tggggaactt tgaccggggc ggcgcttgac cccgcggaag
     5341 gaaaaagcga gtatatgcgg atgatcaggc tgtcacggcg tatgcgtcag ttacctgttc
     5401 accaggaggg gtcccatgtt tcggagcacg ccttccgccc gccgctggtg cgttctcacc
     5461 gcggcgcttg tactctccag cgttcctttt ctgcgcgccg gaaccacggg caaaatcacc
     5521 ggcaccgtgc gccaaccggg cgggcaaccg atcccgggtg tggcggtcca gatttccggc
     5581 cttcgtctcg gcagtatcac cgacgcggag gggcgttatt tcattctgca ggtgcctccc
     5641 ggcacgcacc aggtcgaggc aagcatggtc ggcttccgga ccgcggttac gcagaacgtc
     5701 tcagtgtcgg ccgaccgcac gacgatgctg aatttcaccc tgcaggaaga gaccatcgag
     5761 gtcgaggcac tcgtggtgac tgcgggcagg cctcccatcg aggcagacgt aacgtcctcc
     5821 cagacgacgg tgaacgcccg ccgcgtagca gagattccgg tgaaccagat gcttgacgtg
     5881 ctggcctacg aacccggtgt ttcgctttcg cgcgatacgg agctgaatat ccgcggaggc
     5941 ggcgccacgc aggtccgttt ccaggtggac gggcttgacc gcacggacgg tcttacgaac
     6001 aagccctaca ctcagctcaa ccagacactg gtggccgagg ttacgcttct caccggcggg
     6061 ttcaacgcgg aatacgggaa cgtgcggtcc ggcatggtaa acgtggtgac gaaggacggc
     6121 acggagcgcg gcgttggcaa ggggtgggtc tccggcgtgt tcagcggaac cccgaccgca
     6181 cggaagaagc acttcggccc cggcgcgtac gacgaagcgc agttcgacta ctacacggtc
     6241 atgcggtcag attcgagcac gcgtggcccg accggcgcag cagaccctta cggcgccatc
     6301 tactggccga atctgtacga gtcgacgcgg aatgacaccg ccttccgcag ggtctggacg
     6361 gcggcgccca acatgtataa ggtgttcgac ggctggaatg cccgcgtgac cacggcaaat
     6421 gccaataacc gcggcgccgg agtgtacggt aagggcacgc gcacaaagcc gtggacggcg
     6481 gcgcagatga ttgaggcgtg gcggtacgaa acgaacctga acgagaacgt ctggggatac
     6541 ggcaacaagc cggactggag cctcgacata gcggcggggt ggggtcttcc ccacaaactt
     6601 ggcggaattg tggtcgggta cgtcaccaac aaggaaatga ctgtcgtccc cgcattgcgg
     6661 ccgtattacg tcgaccagac gttcgacgtg aagctgacgc tgacgccgat tgaccggctg
     6721 aagtggagct tttcgtacat gtacggcacg agcgaatcaa ccgggaacgg cacggccggg
     6781 tacgacccgg aaatggcctc caccgcgggt tccgttgcca tcgggagcga ccctgtctcc
     6841 ttccgcactg ccagcgatct tctcggaacc gtgaaccagt caaaaaccgc cgccggcaac
     6901 aaccgtctga atctctctta caacagcctt ctggacggga gtttctggca gtggggtacc
     6961 gcactgacct acacattgag cccgcacacc ttcgccaccg ccgcattcgg gcagacgcgg
     7021 tcgaagtggg atctccagcg ggacatgccc cgggcggaca tcaacgactt cagcccgacc
     7081 ggttcgtacc ggcccccgag cagcttcagc tatggcgcgt ggctgctcat gctgattccg
     7141 ctttccgact ggtccgacat taatggcgat ggaacgcccg accggcccac aagcgctgaa
     7201 gacgccactg cgccgggaag agcttacatg ggcaacccgt acgggtacgg ggcggcgtat
     7261 ccatttgttc cctcgagaac cgtgttcgtg aaccgtcaga tggtgtttac acccggcgat
     7321 acggcagtgg tggtgtcgcc ccaggggtgg gctcaggacg ggtatttcga tctgagcggc
     7381 acattctggc tgggcgaagg cggaacgcaa tggatgactt cgaactccgt tcagaccgca
     7441 gttcgggggg atgttacgca cgtcataggc gaccatacga tcaaagcggg tgcggagttc
     7501 ctcctgagcg atctggaata tcataccgaa caggatgcgc gcctcctcgg aaaccgtttc
     7561 agcgtcaact accgcgatta cggcggcaag tacccggccg cgcgtccgaa cattctgggt
     7621 gtgttcctgc aggataagtt tgagtctcag ggactcatca tgaactacgg cctccggctg
     7681 gaacggtttg acggcggcgc accctactac atgcccgacc tcatttacga cgaccgcctg
     7741 tacagcccgc tgcacgggaa atactggttc gacagcctgc ttgtcgcgcg cgggtggaat
     7801 gtgaacgatc cggcgtgggg cccgctgccg ggcaatcagt acgaggcatg gacgaagttt
     7861 gtgaagaacg gggtaccgga gcagttcccg atgccgtggg acgtgatgaa cgtgttgccc
     7921 cagcgcgacg caaaggcgca ctggcggctt gctccccggt ttggaatctc gcatccggtc
     7981 agcgaccgca cgaagttctt cttcaactac ggcatcttct actcaatggc gaaaccggcg
     8041 cacatgtacg gcacgggtct gcacgatgca cgcatcgggg gaggaggcgg gcggttcgag
     8101 cacatgttca atccggagct gcgccccgca cgcaccacgt cgtacgaggt cggctttgaa
     8161 cacctgttcc cgctgaactt cgtgctcaaa accacgggct acacgaaggc gaacgtggat
     8221 caggtcaccg ggctccaggt gcgcaccggc agcggcgacg ggtaccgcat ttaccggaac
     8281 gcgaactacc aggacattcg cgggctggag atcaagttga gccgcaacgg caggttcgtc
     8341 agcgggtggg caacctacca gatcatcgcc acgcggagcg gccagaccgg gctggaggaa
     8401 ttgcgccaga acattgcggc gtcgagctac tacacgcctt tggtttccac aaactcgccg
     8461 gcggatgtgg tgatggcgtc gatccgcgtg ggaacgcctc tggaatgggg gctgctgcgc
     8521 ggcgggtggg gcgcgagcat cctgcagagc tattccgaaa gcaccggaga gattctgtac
     8581 aacccgaatg ccgttccccg acgggagctt ccgcctgagt acgtgctcag agggcgcgat
     8641 tcgtggggca caagcgcgaa actcacgaag gacatctctc tcccgcgcca gcggcacgtc
     8701 tcgctgtacc tcgatatcaa caaccttttc aacaccaagt acctgaacgg cacctacatg
     8761 aatggcaacg actaccttgc ctacatcgtt gaacggagag cacaggggga gaccgacctg
     8821 cgcgtgggcg atccttccac gtgggatgca ctgacccagc cctaccgcgt gaagaaggcc
     8881 gacggcacct acaccgcgtg gaaagcgccc atcagcccgc gcaccgactg gctcatgttc
     8941 ctgtatccca gatcgtaccg cgcgggtatt cggttcgatc tgtaggacgc gggtagcggt
     9001 tgaagaaaag ggcggccgtg cgttccagca cggccgccct tttctggcac tgcgcgccat
     9061 cacagattct tctttgacgt tggatacata gggtattcgc gattcgcaga acgaggtata
     9121 cgtgtacaac gaacccctcc gcccgcaatt ccatttcacc gcccgcgcgg gctggctcaa
     9181 cgacccgaac ggactcgtgt ttttcgacgg cgtgtaccac atgtttttcc agcacaaccc
     9241 gttcgatacg aagtggggca acatgacgtg ggggcacgcc gtcagcaccg acctcgtcca
     9301 ctggcggcag gtggagaacg ctattctccc cgaccggctc ggaacggcgt tctccggctc
     9361 cgccgcggtg gactggcgga caaccacggg cttcggtgag gccggcgttc cgcccctcgt
     9421 cgcgatgttc acctctgccg gcggcacctc cccggagtcg gcaggccagc cgttcacgca
     9481 atcgatcgcc ttcagcgccg atggcggcac cacgtggcag ccgtacagcg gcaacccggt
     9541 gctgggccac atcgtggaaa gcaaccgtga tccccgcatc ctctggcacg aaccgacgga
     9601 atcgtgggta atggcgctgt acctcgacaa aaacgactac gcgctgttca cctcgtcgaa
     9661 cctgaaaaaa tggacgcgga cgtgcgacgt cagaatcccc gaaacctccg agtgccccga
     9721 cctcttcccc atggcactgg acgacgaccc ggcgcacgtg aagtgggtgt tctggggagc
     9781 gaacggctgt tacctcctgg gcgcgttcga cggcaatacc ttcacaccag aggaaggccc
     9841 gtaccgcctc gactccggcg ccaatttcta cgccgcgcag acgtggagcg acatccctga
     9901 ggcagatggt cgacgcatcc agatcgcgtg gatgaacggc ggccagtacc ccggtatgcc
     9961 cttcaaccag cagatgagct tcccctgcgc gctgtccctg cgcaccacgt cggccggcgt
    10021 tcgcctctgc cagatgccgg tccgtgagat cgaatgcctg cgggaggaca gcgtcatccg
    10081 cacggacgtc gcgctcctcc ccggaaggtt gctcgatgcg tcggcgggcg aactcctcga
    10141 catcgaagcc gagatcgaac cctggcgcgc gaaggcgatc gagtggagca tccgcggcaa
    10201 cgcaattcgg tacgatgcgg agaagcacct gctttcctgc tgcggcaggg aagctcccgt
    10261 tgacctttcg ggcggccttc tgcggctgcg catcctcctc gaccgcacca gcctcgaact
    10321 gttctccggc gacggcaatg tcgggtttca tcaatgcttc cttcccgacg cggcgaacac
    10381 cggcgtcgag ctggccgcga cgggcggtga agcccgcata cgatcgctcg ccgtgcaccg
    10441 actccgctct gcatggtgat acaaaaaact ggagggaccc actgagaacg ccgtccgttc
    10501 ttcgtcgggt accccgccac ctgttctccc cccctcccga caacccgtca tcctggcctg
    10561 acaacctgtc atcctgagcg gagcgaagga tctcttttcg gcgccctcgt tctcgtcatt
    10621 cccgcggagg cgggaatcca tcccaggccg tgtgtgcgtc tcagcggcgc ccgcacccgc
    10681 ccatgcggct accgcaccgc ccgggacgtt gccccgggct gaattatctc ggcccttcag
    10741 gccttacccg ccccacttga tccagccaaa gcgccagcgg cgcgaaatac ccctgcgggc
    10801 gcgcgtcccg tatgcccctc cgtcccactt tgcccaaaca cctatccacc ccctccttcc
    10861 cgggtcgtgt gtggcgtacg tttcagattg cacacaccgg tgtccaccca ccgcatgttc
    10921 ccaaacctcc gggggcaacc atgacatccg ccaccaacac ggcacaagtc acccgctcaa
    10981 tcaattgctt ccgcatcggc acgcctcaat ggctctccga cgagcgcttc gcggccctgc
    11041 tcgcactttt cgaccggtac cccggtgtga ctgacgaagt cacgtttttc acttcggaaa
    11101 cgcacccgcc actccccctt gaggtgctcc gtgaccgctg cgtgctcctt tcgcgccgaa
    11161 tggacgaagt gcgccggcac ggctaccaga ccggcatcaa catccttgcc accatcggcc
    11221 accacgagga aaacctgccc aactccctcg acggcccgta cacgcggatg actgatccgg
    11281 acggacgcat ttcacgcggc gtgctctgcc cgaacgacga gcaggcgcgg gagtatatcc
    11341 gcgaagcgta ccgtctcatc actgaagcga atcccgacta catctgggtg gacgatgatg
    11401 tccgcctgtt cgggcacggg cccattcttg ccgggtgctt ctgcgacacc tgcctcgcgg
    11461 cgttctcccg cgaaacgcgg cgggaatggt cgcgcgagtc cctccgtgag gcggtgaaca
    11521 gcgggccact ggcggaccgg ctgatgtacc gccgcgcgtg gcttgcccac aaccgggcca
    11581 ctatcgcccg ccttttttcc ctgatcgaac agacggtgca tgccatccgc cccggattgc
    11641 ccatcgggtt catgacgggc gagcgctatt tcgaggggta cgacttcgac gcgtgggcgg
    11701 aggtgcttgc gggcccctcg gccccacgcg gcgcgccagt ccggtggcgg ccgggtggcg
    11761 gaacatacac cgacgaaaaa ctcgacaaca tcgtcggcaa ggcgcacgac attgggcgcc
    11821 aggtcgcttt tctgccctcc gccgtaacgg tgattcagtc cgaaatcgag aatttcccgt
    11881 accaacgcct gaagaaaagc gcgaaggcga ccgcgctgga agcggccgcg tatatcgccg
    11941 ccgggtgcac gggggcggcg tacaacgtgc tctccatgta cgacgagccg ctcgacgaat
    12001 acgagcccct tgtgcgcgtc ctgaccgaaa atcggccgtt cctcgacgcg cttgccaaag
    12061 cgggcgggcg ctcgtcgccc gtcggcctgc acagcggctg gtcaaaggac agcttcgcga
    12121 cgttacagct tgccgagggc gactggctct tcccgcggga aggcgttccc ggagggcaga
    12181 cgaacgagtt tcacgaaatc ggcatccccc ccgcgtattc gcttgacgca gcccaggcgg
    12241 tggcgttgac gggaaacggc gtgcgggcgt ttgacagaag aacgcttgag cgcatccttt
    12301 caggcggcgt ctacctcgac gccgaagcag ccagagccct cgaagagatg gggcatgccg
    12361 aactcgtcgg tttccgtgtg ggcgaggcgt ttccgattga cgccattgag caattcctcc
    12421 ccaatccgct ctgtggcgcc tttgccggac gggagcggga cggcaggcag tccttctgga
    12481 aacgcaccgc ctacgccctc gaaccgatgc ccggtgcgga gccgctcgcc cgccttgtgg
    12541 actatggccg gaaggagctc gccgcgtgcg ggctcggggt gtttgagaac tccctcggcg
    12601 gccgtgtctg cgtcgccgga tacgcaccgt ggacgtttct gcagaacctc agcacgtcat
    12661 cgcgcacgaa ggcaatcgtc cgctggctct cgcgggaaac tctgcccgcg tatgtcgagt
    12721 ccttccaccg gatagtgctg tgggtgcggg agcctgagcc cggcaaggtc gccattgccc
    12781 tcctcaacgc atcgctcgac ccggcacggc aggcctcgct cctcgtccgg actgccgcga
    12841 atgaactgcg c
//
LOCUS       DAOSOO010000076        12836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4876_length_12836_cov_4.901651, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000076 DAOSOO010000000
VERSION     DAOSOO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_12836_cov_4.901651"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLT86_07160"
     CDS             <1..896
                     /locus_tag="PLT86_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36425.1"
                     /translation="PRGSPKHAIGGDGAGAMIDVHMHIGKLYVGEKPLTPRHLLRFMD
                     ANDIEKAILLPIESPEEAHYYVSTEYVMRVARKYPDRFVAGCNVDPRIGTGDNNGILR
                     ARLTDYRDRGCKAYGEAMSGLAIDDPALQRVYRICGELRMPVIYHIDGFRNVDEKGFP
                     RFERMLRMFPETVFVGHAQHFWAEISGDVTEAEFSSYPNEPITPGGAVPRLMETYPNL
                     FADLSAGSAYNALTRDPPFGYAFLERFQDRLFFGTDVCRFVQPIDVIPFLKEAREQER
                     ISEIAFEKITRLNAMRVFGLK"
     gene            complement(907..1614)
                     /locus_tag="PLT86_07165"
     CDS             complement(907..1614)
                     /locus_tag="PLT86_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT36426.1"
                     /translation="MVVVGGSGRVGTGVLPYLASWHEVVNFDIRPPSVPAGRYVRGSL
                     FDTELLGRVLTQADGVIFMALGVWPPEVDASYEINVRGVHYVLEAARQAGLMHVVHSS
                     TASVHDESKGSFPDEEMPLEPLHVYGLTKGLGETVCRWFCRMYGMSVLALRLFAPQPY
                     ENWLAQCRTRMPNGWTTFLDTARAYDAALRATSHSGFDAVFISGDWTGTYVNCAKAKR
                     VLGWEPLDRYEGEKQNS"
     gene            complement(1642..2658)
                     /locus_tag="PLT86_07170"
     CDS             complement(1642..2658)
                     /locus_tag="PLT86_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOT36427.1"
                     /translation="MKITGFEFKTVCVPFTWAIQEYWGWTYPTTLIWIHTDEGVTGVG
                     ESNSLHSNITDRKEELQNTYVGKDVFTLDIAQQPFDIQCALYDIAGQALEVPVHALIG
                     RKVRESVELAFWSPPMPPVEMAKEAQRAKGLGFRVHKIKAHRPADTYELVKLITEACG
                     PDYLLRLDPNTHFHDLPTAIRIARSLEGYNIEVYEDPFRFEDMSDYRQFRAKIDVPVA
                     RHLGSPRDVFLWLKNEAMDTFNCGGNVEAMRTFDAIARGAGVPMWGQMFAFGSCIASM
                     FAVHIAATLPACTMALDELPHIRVDDLSGGSFVIEKGAVKVPDTPGLGVQLDMQAVEK
                     YRIR"
     gene            2800..3564
                     /locus_tag="PLT86_07175"
     CDS             2800..3564
                     /locus_tag="PLT86_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT36428.1"
                     /translation="MKIGTVTYNLARSWDIPTIIKNCSETGFEGVELRTTHAHGVELT
                     LSKGERETVRKQFADSPVSIAGLGSVYEYHSTDPEEVRRNVEGSKEYIKLAADVGSPG
                     IKVRPNNAPDEVPLAATLEQIGRALREVAQFGSDYGVEVRLEVHGRITCNPPNIRAIL
                     DHADHRNLKACWNSNDSDMGPDGTIRQNFNLLKHDIGLVHMTDLANPGYPWRELFSGL
                     RSIGYKGFCLAEVPESAEPLRFMRYYRALWMELCNP"
     gene            complement(3706..4959)
                     /gene="hydF"
                     /locus_tag="PLT86_07180"
     CDS             complement(3706..4959)
                     /gene="hydF"
                     /locus_tag="PLT86_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOT36429.1"
                     /translation="MQTTPKSMRLHIGLFGRRNVGKSSILNALTRQDVSIVSPEAGTT
                     TDPVEKAMELLPLGPVLFIDTAGIDDVGALGEKRIAKTTQVFDRTDLAVVITDQPSPG
                     EFENRLIEEFAKRNTPVVFVFNKADLHRATPVALKDPQGRAIPTVTTSVTTGQGISEL
                     LKALITTAPEDFVSTPPIIGDLLPAGELAVLVVPIDKEAPKGRLILPQVQTIRDILDT
                     DAYCIVVKERELRDALERLTRPPKIVVTDSQAFQKVAADVPPSVWLTSFSILFARLKG
                     DLPELVRGALAIEGLQRGDKVLICEACTHHPIQDDIGRVKIPRWLTQYVGAAIEFVHY
                     QGHDFPSDLAPYRLIIHCGGCVHNRREMLTRIALAKRAGVPITNYGLSIAYTLGIFER
                     ALEPFPAAKEVIAAHRQEVTGGKTG"
     gene            complement(5039..>6541)
                     /locus_tag="PLT86_07185"
     CDS             complement(5039..>6541)
                     /locus_tag="PLT86_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36430.1"
                     /translation="VLTASSPDALREAIRQISLGLRKNGTIPLREGMDFRQNPAPADD
                     YTFGFLTNGTGGGAMEPDGTLTLMLTKSAPMADVHLSAPLIPEHGEMVFRYAMFGANG
                     SWRDIGMVRAGFEFDNPLISVRPEVHTGTLPAERSFFSCEAADAVVTAIKPSGNPVPF
                     FQNRSSNPEEGVIVRAYNADGRGSAGRIRMDVPILSARAVNLVEDDPRPMPTVNGTVN
                     WEIGPYAVETIRLERGPACESNRPPVLLGIDAEPFQPVWCRYWQHNLGAHPLGYMPVG
                     IYLDGELPVENSGGAAPTVARLRVTINNNLTDEAIKGKAYLVLPSGWTAVPSEIPYDL
                     APREHACTTATLALEKQNRTGLIKARMEFGGQVYQDVLEVGRRTTRRFVGGGSVRRTG
                     MEIVKEREPEWRVFREGNDILVRVLNPWFEPLEIQTWVISPVETWGKTVGHYALLEVS
                     PRSEGRTIPGRHTETIRFRVSGANGRIPKFWAWVKLACNGKPAYLPVPER"
     assembly_gap    6542..6641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6642..9906)
                     /locus_tag="PLT86_07190"
     CDS             complement(<6642..9906)
                     /locus_tag="PLT86_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT36431.1"
                     /translation="MKGMQYWGGGKPVIPLAALGQGDYVKEWLIIGPFDLSSSFHLRR
                     EQEIGRADAIDLDPIGGERFVKPVEGAAHANAHLPAGSATWRRCSGTPVIDLRRQFPE
                     FPTALAYAATYLCADTDTDLLVSANGTEEYHWSTMTLMLDGVEFGKNDSPALVRVPAG
                     EHLFLLKIGGGVSGQYAWKIDVRMGVLQPLADGLGAGLARLTGFWRGNGAKPRVEVEV
                     VLANREDRILPVAAISAQVNGVEPGPSATDILLAPRTVSSVRLSAPVGNHPQGATVGF
                     SVRCGDRRVAAEGELPELPPDSILHVMQGFHCDPVWLSDQHHYNLVSLDNVRQLLDAC
                     IADPSYRVFLHEIDYLKPFVDEYPDYRAVLFGMIQSGQVALGSSYNQPNEGNCSGEAL
                     IRNILYGCGFHRHFLGGRPRIYHAWDVFGHTPQLSQIVAKSGLSGALWSKPIVGFPPL
                     FRHMALDGTALPHIRTLYGWGTHSIDRLRESTAGLVKEKASFGIKRHLVVDAGDFSCP
                     TAWMVGKSAEMWDSLPKITMTGPEAFLKGVAEDGSKLRVTSRTESQYHVGTVHSRPEM
                     KIANRMGENLLTAAERWATFASLMGAIYPDRALDKAWRQLLFAEHHDALSGTPSDVCY
                     LDLMGGYREALELARDVLRRSTQYIAEATPKPDAGVPVTIFNPLNWSRKGLVTIENSW
                     GDDGITVRALDGTAIPCHVTRKEISFIAPEVPSVGYTTVAVHIVAGDRSEKAAPQAEE
                     NVLENEFWRITLDPQRGGGIVSLVDKETGRELVDREKGVGNDLIALTERGVRGGSPWE
                     FWTTGGRSYASERPASVRIERTCVGDSAVIHGELAGACSFTRTLTVRPNSRTIEAEVI
                     LHDYRRDDVMFVATFPAAIRGALPVVEDRFGTIVTKRNRTAFDYRTGGNHCYSDCVVT
                     PIYNWIASGWSVRVDSGSAALNLGFMGLVIPHDEALDGAIEHLMSAFAHSGVTCTPYF
                     DDDDVPRRKPLEDIYSEPGFEKTKLENTLWNRVDDIALTNQWLAVDVEGNNAYLRSLF
                     GRLPENVEKRIRDVEGAEGWVLAIVEDDEVPHGWPPMPTLVLTASSPDALREAIRQIS
                     "
     gene            10083..10724
                     /locus_tag="PLT86_07195"
     CDS             10083..10724
                     /locus_tag="PLT86_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOT36432.1"
                     /translation="MIPMLPPPNDAVVLFNGKDLSNWKTRDGKPVGWQIANGEIHVIR
                     GTGDIVSDKRFTDFYLHLEFNCPDMPEAKGQAKGNSGVYLQGRYEIQVLDSFGWNVPG
                     KGDCGAIYDQFSPLVNACKPALHWQTYDAVFRAPRFDGNGNRVSGARVTLFHNGRVVH
                     NNVELPGPTGGALDQNVAEPGPLLLQDHGDAVRYRNIWIVPLPLTGSDQYAPA"
     gene            complement(10785..12518)
                     /locus_tag="PLT86_07200"
     CDS             complement(10785..12518)
                     /locus_tag="PLT86_07200"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOT36433.1"
                     /translation="METLRLGLAQINTTVGDISRNTAKVLEWVRRAREEGVDAVIFPE
                     LTTVGYPPEDLLFRTEFLRENLRARDEIVAASEGIAVVFGFVDCPDDIYNAAAIACDG
                     ALCGVYHKRFLPNYGVFDENRYFQAGVAQRVFSIGEFRLGISICEDIWYPGGPADEQA
                     LIGGAHILINISASPYYEGKIGSRDRMVSVRAADNDAVVALVNLVGGQDELVFDGGSI
                     ICSENGHVRALGRQFEEDLICTDVHLDTILRSRLRDPRRRKEKLAALLRREEIPCTPL
                     PVRLRERPVAVPSAPASRLGRTEEVYRALVLGVRDYVGKNGFPGVVVALSGGIDSALT
                     AAIAVDALGKERVAGVTMPSRFNSDETQNDAATVAANLGIRFIQLPIEPVVSAMLDTL
                     RDVFRGLEPDTTEENLQARIRGTLVMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAG
                     GFAAIKDVYKTMVYELCEWRNAQAGYDLIPRTTIARPPSAELRPNQLDQDSLPPYDVL
                     DRILEAYVEMDESLAEITARGFDQAVVSDVMRRVDSSEYKRRQAPPGVKITPRALGKD
                     RRLPITAPRSR"
     gene            complement(12548..>12836)
                     /locus_tag="PLT86_07205"
     CDS             complement(12548..>12836)
                     /locus_tag="PLT86_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36434.1"
                     /translation="FPNWFQPFGQLGVGGGYMEYAGPSLVEHLNAPAGRLGIGANVHI
                     NRRLAIVPTLSYDRLEAASIQAERRYPTMLTLRCSAAFSVFGDNPLLVPGR"
BASE COUNT         2559 a         3803 c         3672 g         2702 t
ORIGIN      
        1 tgccaagggg tagccccaaa cacgcgatcg gtggagacgg agcaggagcc atgatagacg
       61 ttcacatgca tatcgggaaa ctctatgtcg gagaaaaacc cctgaccccc cggcacctgc
      121 tccgattcat ggatgcgaac gacatcgaaa aagcgatcct cctgccgatt gaaagcccgg
      181 aagaagccca ttactacgta tccacggaat acgtcatgcg ggtcgcccgg aaatacccag
      241 accggtttgt cgccggctgc aacgtagacc cgcgcatcgg aaccggtgac aacaacggca
      301 ttctccgcgc gcgcctcacg gattaccggg atagaggatg caaggcgtac ggcgaggcca
      361 tgagcgggct tgcgatagac gacccggctc tgcagcgcgt gtatagaatt tgtggcgaat
      421 tgcgcatgcc ggtaatctac cacattgacg ggttccgtaa cgtggatgaa aagggcttcc
      481 ctcgttttga gcgcatgttg aggatgttcc cggaaacggt tttcgtcggg cacgcgcagc
      541 acttctgggc agagatctca ggtgatgtaa cagaagccga gttcagcagt tatccgaatg
      601 aacctatcac accgggcgga gcggttccgc gcctgatgga aacgtacccc aacctcttcg
      661 ccgacctttc cgcgggaagc gcgtacaacg cacttacccg cgatcctccg ttcggctacg
      721 ccttcctcga gaggtttcag gatcgcttgt tctttggaac ggatgtgtgc cggttcgtcc
      781 agccgatcga cgtgattccg tttttgaaag aagcccgtga acaggaacgt atttccgaga
      841 tcgcattcga gaagatcacc aggctcaacg ccatgcgcgt tttcgggctg aaataacgcg
      901 ggcagactag ctattttgct tctccccttc gtacctgtcg agcggctccc aaccgagcac
      961 gcgcttcgct ttcgcgcagt tcacgtacgt gccggtccag tctccggaaa tgaacacggc
     1021 gtcgaacccg ctgtgtgacg ttgcgcgcaa cgccgcatcg tacgcacgcg cagtatcaag
     1081 aaacgtcgtc catccgttcg gcattctcgt tcggcactgt gccagccagt tttcgtaggg
     1141 ctggggtgca aaaagcctca aggccagcac ggacatgccg tacatgcggc aaaaccatct
     1201 acacactgtt tccccgagac ccttcgtgag tccgtacacg tgcaggggtt ccagaggcat
     1261 ctcctcgtcc gggaaactcc ccttggattc gtcgtggacg gaggcagtac tggaatgcac
     1321 cacgtgcatc agccctgcct gcctggcggc ctccaacacg taatgcacac cccggacgtt
     1381 gatttcgtaa ctggcgtcga cctcaggtgg ccatacgccc aacgccatga atatcacccc
     1441 gtccgcctgc gtgagcacgc ggccgagcag ttccgtgtcg aacagtgatc ccctgacata
     1501 ccgccctgcc ggaaccgacg ggggtctgat atcgaaattg accacctcat gccagctggc
     1561 gaggtagggc agaaccccgg ttccgaccct gcctgacccg cccacaacta ccaccctcaa
     1621 cgcgcgaccc tttcgttttt ctcaacggat tcggtatttc tcaaccgcct gcatgtcaag
     1681 ttgaaccccc aatccgggcg tatcagggac cttcacggca cctttctcga tcacgaaact
     1741 tccgccggag aggtcatcca cgcgtatgtg tggcagttcg tccagcgcca tcgtgcatgc
     1801 cggcagcgtc gccgcgatgt gaaccgcaaa catgctggcg atgcaggagc caaacgcgaa
     1861 catttgtccc cacatgggta cgcccgcgcc ccgggcgatc gcgtcaaatg ttctcatggc
     1921 ttcgacgttt ccaccgcaat tgaacgtatc catggcttca tttttgagcc agagaaacac
     1981 atcccgcgga gagcccagat ggcgtgcgac cgggacgtcg attttcgccc ggaactggcg
     2041 gtagtcggac atatcctcga accgaaatgg gtcttcgtat acctcaatat tatacccctc
     2101 aagagaccgc gcgatccgga tcgcagtggg cagatcgtgg aaatgcgtgt ttggatcgag
     2161 ccttaggagg taatctggcc cacacgcttc cgttatcagt ttcaccagct catacgtgtc
     2221 ggccggacga tgggctttga tcttgtggac acggaagccg agtccttttg ccctttgtgc
     2281 ctctttggcc atttcgacgg gtggcatcgg cggggaccag aaggcgagtt ccacagactc
     2341 ccggactttc cggccgatta acgcgtggac aggaacctcg agcgcctgac cggcgatgtc
     2401 gtacaacgcg cattgaatgt caaaaggctg ctgtgcgata tccagcgtga acacatcctt
     2461 gccgacgtac gtgttctgca gttcttcttt gcgatcggtg atgttgctgt gaagcgagtt
     2521 gctttcgccc acaccggtta cgccttcgtc cgtgtgaatc cagataagtg tggtcgggta
     2581 ggtccatccc cagtactcct ggatcgccca ggtgaacggc acacagacgg tcttgaactc
     2641 gaagccagtg atcttcatcg gggcagatca ttcctttccg ggttgcgctc gttctgaatc
     2701 gtgagcaccg ttgacacgcc cgaacttagt caacacattc ccaagaggca acaccgcgct
     2761 tcgtggaaca agacttgttc gaggcaagag gagcaaatga tgaagatcgg taccgtcacg
     2821 tataatctcg ccagatcgtg ggatatccct acgattatca agaactgctc agaaaccggc
     2881 ttcgagggtg tggaactgcg cacaacgcat gcgcacgggg tggagctgac actttccaag
     2941 ggtgagcgcg aaacggttcg aaagcagttc gccgattcgc cagtttcgat tgccgggctc
     3001 gggagtgtgt atgagtacca ttccacggac cctgaggaag tgcggaggaa tgtggagggg
     3061 agcaaggagt acatcaaact tgcggccgat gtgggctcgc cgggtatcaa agtgcgtccg
     3121 aacaatgcac cggatgaagt tccgctcgcg gcgacgctgg aacagatcgg gcgggcgctt
     3181 cgagaagtgg cccagttcgg ttccgattat ggcgttgaag ttcgactgga agtgcacggg
     3241 aggattacct gcaatccgcc gaacatccgc gccatccttg accacgcgga tcacaggaac
     3301 ttgaaggcgt gctggaactc caacgacagc gatatgggtc cggatgggac gattcgccag
     3361 aacttcaatc ttctgaagca cgacataggt ctcgtccaca tgaccgacct cgcgaacccc
     3421 gggtacccct ggcgcgagct tttttccggt ctgcgttcca tcggctacaa ggggttctgc
     3481 ctcgcggaag ttcccgaaag tgctgagccg ctcaggttca tgaggtatta ccgggccttg
     3541 tggatggagc tttgcaaccc gtaaaagcga acgctggttg aacaggaacg ggcggcgagt
     3601 gggagccaag acgggcttcc actcgccgct tctgatttgt ctgttgcgct caaaaagagg
     3661 cttgtcaggt ctgccctgcg gagggcaaag caagcaggaa gggccttagc cggtcttgcc
     3721 gcccgtgacc tcctgtctgt gcgcggcgat gacttccttt gccgcaggga acggctcgag
     3781 tgcccgctcg aagattccga gtgtgtatgc gattgaaagg ccgtagttcg taataggaac
     3841 gcccgcccgc tttgcgaggg cgattcttgt aagcatctcc cgcctgttgt gaacacaccc
     3901 gccgcaatga atgatcagcc ggtaaggtgc gagatcagag ggaaaatcgt gtccctgata
     3961 atgaacgaat tcaatggcgg cccccacgta ttgggtaagc catcgtggga tcttgaccct
     4021 gccgatgtcg tcctgaatcg ggtggtgagt gcacgcctcg cagatcaaga ccttgtctcc
     4081 gcgttgcagg ccctcgatgg caagcgcacc tcttaccagt tccggcaagt cacccttcag
     4141 acgcgcgaac agaatcgaaa agctggtgag ccagaccgac ggaggcacat ccgctgcgac
     4201 cttctgaaac gcctgcgagt cggtgacaac gattttcggg gggcgcgtga gacgctccag
     4261 agcatcccgc agctcccttt ccttcacgac aatgcagtag gcgtcggtgt caagaatgtc
     4321 ccggattgtt tgaacctggg ggaggatgag tcgccctttc ggagcttcct tgtcgatcgg
     4381 tacgaccaga accgccagtt cgccggccgg cagaaggtcc cctatgatcg gcggggtgct
     4441 gacaaaatcc tccggcgcag tagtaatgag tgccttcagc agctcagaaa taccctgccc
     4501 tgttgttacg gacgtcgtga ctgtcggaat cgcacgtccc tgagggtcct tcagcgcgac
     4561 gggagtcgcg cgatgtagat cggctttgtt gaatacgaaa accacggggg tgttccgttt
     4621 cgcaaactcc tcgatgaggc ggttttcaaa ttcgccgggg ctcggctgat cagtgatgac
     4681 gacggcgagg tcggtgcggt cgaagacttg tgttgttttt gcgatcctct tctcacccag
     4741 agcgcctacg tcgtctatgc cggccgtatc gatgaaaaga accggtccca ggggaagcag
     4801 ctccattgct ttttccaccg ggtcagtggt ggtgccggcc tccggactga cgatagacac
     4861 gtcctgccgt gtcaacgcgt taagaatgct cgatttccct acgttcctcc gaccgaacag
     4921 gccgatatgc agccgcatgc tttttggcgt ggtttgcaat tcaagcgtct cctttgtgtc
     4981 gattagtgcg cgcggccgtg gggtccgatg aaggtacccc gccgggcgaa cgcgagagtc
     5041 atctctccgg caccggcagg tatgctggtt tcccgttgca cgcaagtttg acccaggccc
     5101 agaatttcgg aatgcgacca ttggcacccg aaacccggaa acggatcgtc tctgtgtgcc
     5161 gcccggggat tgtccgccct tcggaccgcg gtgaaacttc cagcagcgcg tagtgcccga
     5221 ccgtttttcc ccacgtctcg acgggcgaga taacccaggt ctgtatctcc agaggctcga
     5281 accatggatt gagaacccga accagtatat cgttgccttc tcggaacacc ctccattcgg
     5341 gttccctctc tttcacgatt tccattccgg tgcgccgaac gctgccgcca ccgacaaatc
     5401 gtcgagtcgt ccttcttccg acctcgagca cgtcttgata gacctgaccg ccgaactcca
     5461 tgcgcgcctt gatgagcccc gtgcgatttt gcttttcaag agcgagcgtg gctgttgtac
     5521 aggcgtgctc tctcggtgcg agatcgtagg ggatttctga aggaactgcc gtccatcctg
     5581 atgggagaac cagatatgcc ttccctttga tggcctcgtc cgtgaggttg ttgttaattg
     5641 ttacgcgcaa ccgcgcaacg gtcggagccg cgccgccgga gttctcgacg ggcaattcac
     5701 cgtcgaggta gatacccacc ggcatatacc caagaggatg tgctccaaga ttgtgctgcc
     5761 aatagcgaca ccacacaggc tggaaaggtt ccgcgtcgat cccgagcagc acgggcgggc
     5821 ggttcgattc gcacgcaggg ccgcgttcga ggcggatggt ttccaccgca tacggcccta
     5881 tttcccagtt gacggttccg ttgaccgttg gcatcggtct ggggtcgtcc tctaccagat
     5941 tcacggctct ggcggacagg atcggaacgt ccattcggat acggccagcg ctgcctcgcc
     6001 cgtcggcgtt gtatgcgcgc actatgacac cttcctccgg gttcgaggaa cggttctgga
     6061 aaaacggaac gggattccca ctcggcttga tcgctgtcac gacggcatcc gcggcttcgc
     6121 aggagaaaaa gctccgctca gcggggagtg tccccgtgtg aacctccgga cgaactgaga
     6181 tcagcgggtt atcaaactca aatccggccc gaaccatgcc gatatcgcgc caagacccgt
     6241 ttgcaccgaa catggcgtac ctgaagacca tttccccgtg ttctggaatc aacggcgcgc
     6301 tcaggtggac gtccgccatc ggagccgact ttgtgagcat gagtgtgagg gtcccgtccg
     6361 gctccatggc gccgccgccc gtgccgttcg tgagaaaacc aaaagtgtag tcgtccgccg
     6421 gagccgggtt ctggcggaaa tccattcctt cccgcagggg gatcgtgccg ttcttccgca
     6481 agcctaggct gatctgccgg atcgcttctc gaagtgcgtc cgggctggag gctgtcagca
     6541 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggctgatct gccggatcgc
     6661 ttctcgaagt gcgtccgggc tggaggctgt cagcaccaga gtcggcatgg gcggccaccc
     6721 atgcgggacc tcatcgtctt cgacgatcgc cagtacccaa ccctccgcgc cttccacgtc
     6781 ccggattctc ttttccacgt tttcggggag tcttccaaag agcgacctta agtacgcgtt
     6841 gttaccttca acatccacgg caagccactg gtttgtaagc gcgatatcat ccacccggtt
     6901 ccagagagtg ttttccagtt tcgtcttctc aaaacccggc tcggaataga tatcctcaag
     6961 cggttttctc cttggaacgt catcgtcatc gaaataaggt gtacacgtca cgccgctgtg
     7021 agcgaacgca gacatcaggt gttcgattgc tccgtcgagt gcttcgtcgt gaggaatgac
     7081 aagccccatg aacccgagat tcagggcggc gctgccagaa tcaacccgta cagaccaccc
     7141 gctcgcgatc cagttgtaga tcggtgtcac cacgcaatca gagtagcaat ggttgccgcc
     7201 tgtgcggtag tcaaaggccg tgcgattccg cttcgtcacg atggttccga atctgtcttc
     7261 gaccacggga agcgcgccgc gtattgcggc agggaaagtc gctacgaaca tcacgtcgtc
     7321 gcgccggtag tcgtggagaa ttacctcagc ttcaatggtc cggctgttcg gcctcactgt
     7381 cagtgttcgg gtgaacgaac acgcaccggc cagttcgccg tgtattaccg cggagtctcc
     7441 cacgcaggtt cgttcgatcc gaaccgaggc cggtcgctcc gaggcgtagc tccttccgcc
     7501 tgttgtccag aattcccacg gcgatccacc cctgacgccg cgttcagtga gcgcgatgag
     7561 gtcgtttcct accccttttt cgcgatccac gagctcccgc cccgtctctt tgtcaacgag
     7621 gctcacaatt ccaccgccgc gctgcgggtc gagagtgatt cgccagaact cgttttcgag
     7681 cacgttctct tccgcctgtg gggcggcttt ttcgctgcgg tcgccagcca cgatgtgaac
     7741 tgccaccgtg gtgtatccca cactcggtac ctcaggagcg atgaatgaga tctccttccg
     7801 ggtaacgtgg cacgggatgg cggttccatc caacgcccga accgtaatcc cgtcgtctcc
     7861 ccacgaattt tcaatggtca cgagaccctt gcgagaccag ttgagagggt tgaaaatcgt
     7921 caccggtacc ccggcgtcag gcttcggggt tgcttcagcg atgtattggg tggatcggcg
     7981 cagtacgtcg cgcgcaagtt ccagcgcttc ccggtacccg cccatgagat caaggtagca
     8041 gacgtcagaa ggagtgcctg acaacgcgtc gtgatgctcc gcgaaaagaa gctggcgcca
     8101 cgccttgtcg agggccctgt caggataaat tgcacccatc agagaggcaa aggttgccca
     8161 gcgttccgcg gctgtcaaca gattctctcc catccggttc gcgattttca tctccgggcg
     8221 tgagtgcacc gttccgacgt ggtactggga ttccgtcctg cttgtgacgc ggagctttga
     8281 cccgtcttca gcaacgcctt tcaggaaagc ttcgggtcct gtcatggtga ttttcggcaa
     8341 ggagtcccac atttcagcac ttttgcccac catccaggca gtggggcaac tgaagtcacc
     8401 ggcgtcgacc accagatggc gcttgatacc gaaggatgcc ttttccttca ccaatccggc
     8461 cgtggattcc cgcaggcggt ctatgctgtg cgtgccccag ccgtagagcg tacggatgtg
     8521 cggcaacgcg gtgccgtcca gagccatgtg ccgaaacagt ggtggaaatc cgacgatggg
     8581 tttggaccac agagctccgc tcaggccaga ttttgcgacg atctggctga gctgaggggt
     8641 atgcccgaat acgtcccacg catggtaaat ccttggcctg cctccgagga agtgccgatg
     8701 gaatccgcac ccgtaaagga tgttccggat cagcgcttcg cccgagcagt tgccttcgtt
     8761 gggctggttg tacgagctcc cgagagcaac ctgcccgctc tggatcatcc cgaacaggac
     8821 cgcgcggtaa tccgggtatt cgtcaacaaa cggtttcagg tagtctatct cgtgaagaaa
     8881 cacgcgataa ctgggatccg ctatgcaggc gtccagaagt tgcctgacgt tgtcgaggga
     8941 gacgaggttg taatggtgct gatcgctcaa ccagacggga tcgcagtgga atccctgcat
     9001 cacatgcaag atcgagtcag ggggcaattc gggaagttcg ccttccgccg ctacccttct
     9061 atccccgcag cggaccgaga agcccactgt ggcaccctga ggatgattac cgacgggtgc
     9121 cgaaagcctc acagacgaga ccgtgcgcgg cgcaagcagg atatccgtcg ccgaaggccc
     9181 gggttcgacc ccgttcacct gggccgaaat ggcggcgacg ggcaagattc ggtcttcccg
     9241 attggcgagc actacctcga cttcaacgcg tggctttgca ccgtttcccc tccagaatcc
     9301 cgtcagtcgg gcaagaccgg cccccagccc atccgcaagc ggctgcaaaa cccccatgcg
     9361 aacatcaatt ttccatgcgt actgtccact gacacctcca ccgattttca acaggaaaag
     9421 gtgctctccg gcagggacac gcaccagcgc cggggaatcg tttttcccga actcgactcc
     9481 gtccagcatg agcgtcatag ttgaccagtg atactcctcc gtcccgttgg ccgaaacgag
     9541 caggtccgtg tctgtgtcgg cgcacaggta tgtggcggcg tacgcaaggg cggtggggaa
     9601 ttccgggaat tgacgtctga ggtcgatcac gggagtgccg ctgcacctcc gccacgtggc
     9661 ggagccggcc ggaaggtgcg cgttcgcgtg agcggcccct tccacaggtt tcacgaatct
     9721 ctctcctcca atgggatcga gatctattgc gtcggcgcgg ccgatttcct gttcacgccg
     9781 aagatggaag ctggaggaga ggtcaaacgg cccgattatc agccattctt tcacgtaatc
     9841 tccctgaccg agggcggcga gagggatcac cggtttcccg cctccccagt attgcatgcc
     9901 tttcattcgt tgctccctgt ttgtccgcag aaggtgacga tgcttttcat ttggcgactc
     9961 tcccgcaccg aagggtggct acgggcgaca aacgtttggt tctgtagaaa tcggttcgta
    10021 cgttttcagc gttgcgtcag gtacgagcag ggctggaacc gttgctcttg aaaggagaaa
    10081 ccatgattcc catgcttccc ccacccaacg acgccgttgt gcttttcaac ggcaaagatc
    10141 tctccaactg gaagaccagg gacggaaaac ccgtcggctg gcagatcgcc aacggcgaaa
    10201 tccacgtcat tcgcggaacc ggtgacattg tgtccgacaa acggttcacg gatttctacc
    10261 tgcatctcga attcaactgc cctgacatgc cggaggcgaa agggcaggca aaaggcaaca
    10321 gcggcgtata tctccaagga cgatacgaga tacaggtgct ggattctttc gggtggaatg
    10381 tgcccggaaa aggtgattgc ggcgcaatct atgatcagtt cagtcctctg gtgaacgcct
    10441 gcaaaccggc actgcactgg cagacgtacg atgcggtctt ccgcgcaccg cggtttgatg
    10501 gcaacggaaa ccgtgtaagc ggcgcccggg tcactctttt ccacaacggc cgagtggtgc
    10561 acaacaacgt cgaattgccg ggcccgaccg gaggggctct ggaccagaat gtggcagaac
    10621 ctggtcccct tctcctgcag gaccacgggg acgcagtgcg ctaccggaat atctggatcg
    10681 ttccgcttcc tctgacaggc tccgaccagt acgccccggc gtaggaaagc ctcgggttct
    10741 ttcgggcgcc tcgtcggtcc aacttgaccg gcgaggcgct tcgtttatcg ggatcgcggt
    10801 gcggtgatcg gaagtcggcg atcctttccg agtgcccgcg gcgtgatttt gactcctggg
    10861 ggcgcctggc gacgcttgta ttcgctggaa tcaactctgc gcattacgtc gcttacgacc
    10921 gcttgatcga aaccacgcgc tgtgatttcc gcgagagact catccatctc cacatacgcc
    10981 tcgagtatgc ggtcgagcac gtcgtacggg ggaaggctat cctgatcgag ctgattcggc
    11041 cggagctctg ctgagggtgg acgagcgatg gttgtgcggg ggatcaggtc ataaccagcc
    11101 tgggcgtttc tccattcgca aagctcgtat accatcgttt tgtagacgtc tttgattgcc
    11161 gcgaacccgc ccgccatgtc accgtacaac gtggcgtacc caactgacat ttcgctcttg
    11221 ttccccgtag tcagtacaag ccacccgaac ttgtttgaaa gcgccatcac cagtgttccg
    11281 cgtatgcggg cttgcaggtt ctcttcggtg gtgtccggct ccaggccacg gaagacgtct
    11341 cgcaaagtgt caagcatggc ggacaccaca ggttcgatag gaagctggat gaagcgaatt
    11401 ccgaggtttg ccgcgacagt ggctgcgtca ttctgtgtct cgtctgagtt gaaccgtgac
    11461 ggcatcgtga ctccagccac gcgttctttc cccagtgcat ccaccgcgat ggccgccgta
    11521 agagctgagt ctattcctcc ggacaatgct acgacgacgc cggggaaccc gtttttccct
    11581 acgtagtctc gcaccccgag cactagtgcg cggtacacct cttccgtcct gccgagtcgc
    11641 gaggcaggcg cggatggaac ggcgacagga cgttcgcgga gccgcacggg cagcggcgta
    11701 cacgggattt cctctctccg cagaagcgcg gccagtttct ccttgcgccg ccgcgggtca
    11761 cgcaggcgcg accgcagaat cgtatcgagg tgtacatccg tgcagatgag atcctcctca
    11821 aattgcctcc cgagggcgcg cacatgccca ttttccgagc agattatgct ccctccgtcg
    11881 aaaaccagtt catcctggcc accgaccagg ttgaccagag ccactaccgc gtcattgtcc
    11941 gctgctctga cggacaccat gcggtcgcgg cttccgatct tcccttcgta atacggtgag
    12001 gctgagatgt tgatgaggat gtgtgcgccc ccgatcagcg cctgttcatc ggcaggtccg
    12061 ccggggtacc agatgtcctc gcagatgctg ataccaagtc tgaattcccc gatggagaaa
    12121 acccgttgcg cgactcccgc ctggaaatac ctgttttcat cgaatacgcc gtagttcggg
    12181 agaaatcgtt tgtggtacac gccgcaaagg gcaccatcgc atgcaatggc ggcggcgttg
    12241 taaatatcat cagggcagtc tacgaagccg aacacaaccg ctataccctc acttgcggca
    12301 acgatttcat cgcgtgcccg cagattctcg cgcagaaact ccgtccggaa gaggaggtct
    12361 tccggcgggt atcccacagt agtcagttcg gggaaaatca ccgcgtcgac gccttcctcc
    12421 cgcgcacggc ggacccattc aagcactttg gcggtgttcc tcgagatgtc gccgacggtg
    12481 gtgttgattt gggcaaggcc aagacgaaga gtttccatca gtccatcact ccgtgtcgtg
    12541 ccccggatca tctccccgga acaagaagcg ggttgtcgcc aaaaaccgaa aacgcggcgg
    12601 agcagcgcag tgtaagcatt gtggggtatc gacgttcggc ctggattgaa gcggcttcaa
    12661 ggcgatcgta cgacaacgtg ggaacgatgg cgagccgtct gttgatgtgc acattggcac
    12721 ctatgccgag gcggcccgca ggtgcgttca gatgctcaac cagtgatggt cctgcgtatt
    12781 ccatatatcc cccgcctacg cccaactggc cgaatggttg gaaccagttc gggaac
//
LOCUS       DAOSOO010000077        12740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4921_length_12740_cov_6.271502, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000077 DAOSOO010000000
VERSION     DAOSOO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_12740_cov_6.271502"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLT86_07210"
     CDS             <1..759
                     /locus_tag="PLT86_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36435.1"
                     /translation="AEILQFVVGRREVFSGGRLAVAVPESLTVTGTLGETVPRDLSIQ
                     AVGDVTGNWRQTAKPALFDAELVKIGSSDGAAWRLYTESPVFSVIAVFEGGSVVPEFP
                     SDWLVETVRSADQVLIAAAGARPLSPGAVVVRSTSGEVRFVKGSVNEEASLRSVGNPT
                     PAQVTLSVSPNPFNPRCTIRFDCPEPVQVRLSVYTVTGQFVRELAAGHRDAGSFSEVW
                     DGRDHSGAPVASGVYLVRLDAGSSRKLVRVVLSR"
     gene            913..1587
                     /locus_tag="PLT86_07215"
     CDS             913..1587
                     /locus_tag="PLT86_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HOT36436.1"
                     /translation="MARSILALFSHSPWGAIQEHMRAVDKCVQLLDPLMDSVLAGNYP
                     RTEQLAREISALENAADEIKNELRDHLPRSIFLPVARQDLLDILSTQDGISDAAEDVG
                     MIVTMRKMEVQPALVDLLDPFVQKTLESVASYSQVIGELDRLVEASFVGKEAERVTEL
                     IRKVGRLEHEADNAQEALSRRLFEIEDSMKPGALFMWIEIIRRLGSVANRAEKAANRL
                     RMFLAH"
     gene            1627..2874
                     /locus_tag="PLT86_07220"
     CDS             1627..2874
                     /locus_tag="PLT86_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT36437.1"
                     /translation="MPDPVILAIFGLVAGCYLAWTIGANDVANAMGTAVGSRVLTLGA
                     AVAVASVFEFSGAFFAGGQVTNTIRYEVINVPLVEKVGPFMGVGMLAALLASAVWLHI
                     ATWKGWPVSTTHSIIGAVAGFGFAASGGDAVRWMTLAGIGTSWVLSPFLGGLFAWVLF
                     RVIRRAVFAHDHPARRARMVTPLLIGVTGAVLTLSMVYKGLANLHLDLPIGHALALAG
                     CVGVLSAVGARVVLGRRQDHGADRDTQSRNVERQFRPLLVITACYVSFAHGSNDVANA
                     VGPMAAVVVSSKAGFLSYFHDPATILMALGGAGIVVGLATYGYKVIETIGKKITEVTP
                     SRGFVIQFSTASITLLGSKLGLPVSTTHIIIGAVVGVGFARGLSAVNTGMVRQIVWSW
                     LITFPAAGLLCVVFFHVLRPLFL"
     gene            2984..4120
                     /locus_tag="PLT86_07225"
     CDS             2984..4120
                     /locus_tag="PLT86_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36438.1"
                     /translation="MAIGTCFPLGMYELPRNEEEWAHWKSAGINLLPCASREDLDSVH
                     RHGMKGWVSLPMVLREDDDGAALRAAVSALKDHPALYVWEAPDEIIWNTNRRVTGVLA
                     SFWWDQPERMRKELAQEYDALVLGLERGSAIVRSLDPGRPIWLNEAARSDMEIVSRCL
                     PFLDIISTDFYPVLPQTPDPRRARIRTHDMIALLCDRFRATAPDHEFWFVQQAFSWHR
                     LERKRHPDVPIAYPTRCDYRFMAWQAIAHGARGLLWYGSAHEDRPAPFLDDLMAVVRE
                     LSELSPFLLSEHAPVLEVRVDTKQYPPTRGVSCFMREFGGRTFLALVNEDPYENDVLV
                     TGVPGEEITGWTAFGDSERPHVPIDNGFVVPLSGREVRLMMSAA"
     gene            complement(4315..5862)
                     /gene="rmuC"
                     /locus_tag="PLT86_07230"
     CDS             complement(4315..5862)
                     /gene="rmuC"
                     /locus_tag="PLT86_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT36439.1"
                     /translation="MLALPALLAFIVGLLIGVAAALLVTRSRIERARTQAATEAATAR
                     AVLEERLRSTDELAASLRGQLAVLQDDLRHRTEQLQQEAERRSAAEAQAARIPELESS
                     LEALRASNADLEKIRSELETRIAEERKAADEKMAMLQQARTELSDAFKALSSEALNSN
                     NKAFLELAKTHLEKFQEGAKTDLEARQKAVEELVKPVKESLVSMDTKLQELETKRVSA
                     YAELLTQVNAMKDQQSQLRNETSRLVQALRTPNVRGRWGEIQLRRVVEMAGMLNYCDF
                     DEQVTAKEGEIRPDMIIRLPGGRNIVVDAKTPILAYLDAIETDNEEKRKEHLARQAQH
                     VREHIKKLSAKSYWDQFDNTPEFVVLFLPAESLFAVALEQDRTLIEEGVKQRVIPASP
                     ITLIALLRAVSYGWRQEDIAKSAEEIAALGKELYDRIANMADHLDRLGDRLKSAVDSY
                     NKAIGSFETRVLVSARRFKELKAAAGNEIKEIPQLDIVPRTVDIPDFPAPRLSPPAPD
                     NELPAGA"
     gene            complement(5876..6361)
                     /locus_tag="PLT86_07235"
     CDS             complement(5876..6361)
                     /locus_tag="PLT86_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36440.1"
                     /translation="MSPRAIVALAVLGFSFLAPSFVSAATRYGGWDKSVYFGPFAVGY
                     ATTKPFSGGGTPQLRWGWKVEGGWTGGGFGGASTYNDALIVSPQGLIRLAIPLEGTMV
                     LYPQLGLGVAARFDDGSGTHSGAWVGPIGSFGIGLQFGKWGALIQGPGLPGIGVTYHF
                     Q"
     gene            6452..7360
                     /locus_tag="PLT86_07240"
     CDS             6452..7360
                     /locus_tag="PLT86_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT36441.1"
                     /translation="MKKLSVVALICGLFAACSHSANLSRALGSVNERRAFGYLTAQTA
                     FGPRNPGSRGHAACRDYLIRQLNALTDTVELQHFQPIDSRGNRTPPMTNIIGRIAPHQ
                     NRRYLLCAHWDTRPRADRDPDPRRRDLPIPGANDGASGVAVLLEIATVLKRVSPPVGI
                     DIVFFDGEDYGEEGRLEDYFLGSREFARRWRGQKHEMAILVDMVGDRNLQLPVEAFSW
                     QAAPEVVDRVWGAAERLGERAFIRSMGVEIQDDHVPLIEAGWRAIDVIDFSYPYWHTH
                     ADTPDKCSPQSLGSVARVLLAAIIER"
     gene            7403..7834
                     /locus_tag="PLT86_07245"
     CDS             7403..7834
                     /locus_tag="PLT86_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOT36442.1"
                     /translation="MCANERRFAFSRRELAALAALTVSLAVGCFLVWLIPEPSDLRVV
                     RSPELASLAREVAPSTGSHLININTASFDELVSLPGIGPKKAQAILDYRHANGRFPDV
                     GAIERVKGIGPATVARLRPLITVQDRAPAGERSSPATGGSR"
     gene            7831..8142
                     /locus_tag="PLT86_07250"
     CDS             7831..8142
                     /locus_tag="PLT86_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36443.1"
                     /translation="MRRKAMPVAVRLVIAVIAFSAAAIPPLIRYSAAEEAEECARRRE
                     AVRTAASRLPDERWWNERGCPRDGENYRFAVGQDSTVEVVCPKGHGIQKLPVHRWFSP
                     F"
     gene            8168..8653
                     /gene="coaD"
                     /locus_tag="PLT86_07255"
     CDS             8168..8653
                     /gene="coaD"
                     /locus_tag="PLT86_07255"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT36444.1"
                     /translation="MRRALYPGTFDPITLGHLDIIERSLRIFDEVVVSVADTRPGVVF
                     PVEDRVRLVEAATKHLDRVRVVSFRALLVHHWQSLGKPTVVRGLRAVQDFEAEFAMAL
                     GNRRLADDFEIIFLMPRNDFVFLSASIVREVARWGGDVSSMVSPPVAEYLATRYGPPA
                     K"
     gene            8703..9704
                     /gene="pta"
                     /locus_tag="PLT86_07260"
     CDS             8703..9704
                     /gene="pta"
                     /locus_tag="PLT86_07260"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT36445.1"
                     /translation="MSSTVIQQIRERAAAHKGRIILSEGNELRAIQAARKLLDDGICE
                     VTLVGAPAEIGKVAKEGGVRVSGITMVDPSEHPMREQFAAELYEKRKAKGLTKEQAEE
                     LSRRQIYCAAMMVSHGEGTGTVGGAVHATAEIVRAGLWCIGMAKDVSVVSGAFLMVVP
                     DFLGTGKDKTLYYADSGVVPDPNSDQLASIAIASAATFRALTGEEPRIAMLSFSTKGS
                     ATHPDVDKVIVATKKVQELRPDLLIDGELQADAALVPGVAKAKAPGSSVAGTANVLIF
                     PDLGAGNIGYKLTQRLAHATALGPILQGLAKPAHDLSRGCSAEDIVDMAAIAICMAE"
     gene            complement(9783..10910)
                     /locus_tag="PLT86_07265"
     CDS             complement(9783..10910)
                     /locus_tag="PLT86_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36446.1"
                     /translation="MSERVQMFDPKTGIQVVQLMSYPTPSIPLMYDWPSVTPDNRRVV
                     FLVQRAASREAPWDIYRCDTDGLDPRQLTRKEDTAGYPNVTLSLDGKSAYVLWRDEKI
                     LRKLDIETGAMEDLIAVGNHCAPGMIVCGVRYGSVGSRLYLDLRDYAAGEGRMIRIDL
                     ATGAVTANDTNMTIAAVDQATGRLIVTKNFMKLGTRITENGNRVFHNVNPEPMRYYSV
                     NEDGSDEQYICDVGMFAHSTILGNTSKIQGPSQPPHRCIWIAEAGTEPYKLVEGPYFW
                     HAGASYDAEWILSDTNWPDQGLQLVHVPSRHFRTLCHAGATQGHAQWGHPHPGLSQDG
                     RIGVFTSDRTGMAQVYVAHITEEFRESVIAGQLDNPKDKWI"
     gene            complement(10981..12111)
                     /locus_tag="PLT86_07270"
     CDS             complement(10981..12111)
                     /locus_tag="PLT86_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36447.1"
                     /translation="MQSERVQMTDPKTGIQVIQLTSYPTPSIPLNYDWPSVTPDNRRV
                     VFLAQRSAEREAPWDIYRCDTDGLDLVRLTKKDASAGYPDVTLSLDGKSAYVIWREEK
                     ILRKMDMETGAMEDLLSVADHCPPGLAVLAVRYGSLGSRLYLDLRDCMAGEGRMLRVD
                     LATGAVTANDTNMTIAAVDQATGRLIVIKNFMKLGTKMGADGARVFHNANPEPMRYYS
                     VKEDGTDEQYIADVDMYAHSTILGKTSQIQGPSQPPHRCIWISEAGKVPYKLVEGPYF
                     WHAGASYDAEWILSDTNWPDQGLQLVHVPSRNFRTVCYSGATLGHSQWGHSHPGLSQD
                     GRIGVFASDRTGITQVYVALITDEFRESVVAGQLDNPKDKWI"
     gene            12290..>12740
                     /gene="hisG"
                     /locus_tag="PLT86_07275"
     CDS             12290..>12740
                     /gene="hisG"
                     /locus_tag="PLT86_07275"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT36448.1"
                     /translation="MAQFVTFGLPKGSLQEATLDLMKRAGFEFTVSSRSYFPTCDDPE
                     VKAVMFRAQEIAKYTERGVMDVGLTGLDWILDSGADVVEVADLIYSKVTTQKARWVLA
                     VPENSPIKEAKDLQGKRIATELVHVVEAYLKRHGVKAEVEYSWGATEV"
BASE COUNT         2305 a         3677 c         4062 g         2696 t
ORIGIN      
        1 gcggagattc tgcagttcgt ggtggggcga cgcgaggttt tttccggggg gcgccttgcg
       61 gttgccgttc cggagtcgct cactgtcacg ggcacgctcg gcgaaacagt gccgcgggac
      121 ctttccattc aggcagtggg cgatgtgacg gggaactggc ggcagaccgc aaagcccgcc
      181 ctgtttgacg cggaactggt gaaaatcggt tcgtcggacg gcgcggcgtg gcgcctgtac
      241 accgaaagtc ccgttttctc cgtaatcgct gtttttgaag gaggttctgt cgtacccgag
      301 tttccaagcg actggctggt tgaaaccgtc cggtcggcgg atcaggttct gatcgccgca
      361 gccggagcgc ggccgctctc accgggagcc gttgtcgtgc gttcaacgag cggagaagtg
      421 cgctttgtga agggttcggt gaacgaggaa gcgtctctcc gttccgttgg taatccgacg
      481 ccggcacagg tcaccctgtc cgtttcgcca aacccgttca acccccggtg tactatccgg
      541 ttcgactgcc ctgagccggt gcaggtgcgc ctttctgtct acacggtgac gggccagttc
      601 gtgcgcgaac tcgctgcagg gcaccgcgac gcgggaagct tttcggaagt atgggacggg
      661 cgcgatcatt ctggcgcacc cgtggcaagc ggagtgtacc tcgtccggtt ggatgcaggt
      721 tcgagccgga aactcgtccg ggtggtactc tcccgttgag cagaatcccg ctggcgggtc
      781 cgttccccac gggagcgagg aaaaccgacg ggaccgagcg cagcctgcca acgcatttct
      841 ccgcaggtcg gctttccgta tactttccgt gaacacgttg cgactcgcct ttaccttccg
      901 agggggtgtg ccatggctcg ctctattctt gccctgtttt cgcattctcc gtggggcgcc
      961 attcaggagc acatgcgcgc agtggacaag tgcgtgcaac tgctggatcc gctcatggat
     1021 tccgttctgg ccggcaatta ccctcgcacg gaacagctcg cacgtgagat ttccgccctc
     1081 gaaaacgccg ccgatgagat caaaaacgaa ctgcgggatc atctgcctcg aagtatcttc
     1141 cttccggtgg caagacagga cctgctcgac atactttcca cgcaggacgg catttccgac
     1201 gcggcggagg atgtcggaat gatcgtcacg atgcggaaga tggaagtcca gcctgcgctc
     1261 gtggatctgc tggatccctt tgttcagaaa acgctggaat cggtggcctc ctactcgcag
     1321 gtaatcggcg aactggatcg tcttgtcgag gcctcgttcg tcggcaagga agccgagcgc
     1381 gttacggaac tgattcggaa ggtaggcaga ctggaacacg aagcagacaa cgcgcaggaa
     1441 gcgctttcgc gcaggttgtt cgagatcgag gattcgatga aacctggcgc gttattcatg
     1501 tggatcgaga tcatccgccg tcttggaagt gtggcgaaca gggcggaaaa agccgccaac
     1561 aggcttcgaa tgtttctcgc gcactgacat caaccgcttt cgttcccccg aatccaggag
     1621 cccgtcgtgc cggatcccgt catcctcgcc atttttggcc tggttgccgg gtgctatctg
     1681 gcgtggacta ttggcgcgaa cgacgttgcc aacgcgatgg gaaccgccgt cggatctcgt
     1741 gttctcactc tgggtgccgc ggtagctgtt gcatcggttt ttgagttttc cggtgccttt
     1801 ttcgccggcg ggcaggttac caacacgatc cggtacgagg tgataaacgt ccctcttgtg
     1861 gagaaagttg gccctttcat gggagtcggc atgctcgccg cacttctggc gtcggcagtc
     1921 tggctccaca ttgcgacgtg gaaggggtgg cctgtgtcca cgacacactc catcatcggc
     1981 gcggtggcgg ggttcggatt cgcggcgagc ggcggggatg cagttcgctg gatgacactg
     2041 gccggaatcg ggacgagctg ggtactgtca ccgttcctcg ggggtctttt tgcctgggtg
     2101 cttttccggg tgatacggag ggcggtgttt gcgcacgacc atcctgcaag acgcgcgaga
     2161 atggtcacac cgttgctcat cggtgtgacc ggcgcggtat tgaccctctc catggtctac
     2221 aaggggctgg cgaacctcca tcttgatctc cccatcggcc acgcgcttgc gctggcggga
     2281 tgcgtgggtg ttctttccgc cgtgggcgcg cgggttgttc tgggacggcg acaagaccac
     2341 ggagcggaca gggataccca atcccgcaac gtggaacgcc agttccgtcc gctgctcgtg
     2401 attacggcgt gctacgtgtc gttcgctcac ggttcgaatg atgtagccaa cgcggtcggg
     2461 ccgatggccg ccgttgtggt gtcgagcaaa gccggatttt tgagctattt ccatgaccct
     2521 gcgacgattc tgatggccct cggcggagca ggcatcgtag tcggtcttgc gacgtacggc
     2581 tacaaggtga tagaaaccat cgggaagaag attacggaag tgactccgag ccgtggtttc
     2641 gtcattcagt tcagcactgc atccataacg ctgctcgggt cgaaactggg gctgcctgtg
     2701 tcgacaaccc acatcatcat cggggcggta gtgggcgtgg ggtttgcccg agggctgtcc
     2761 gcggtcaaca cgggaatggt gcgacaaatc gtgtggtcgt ggctgattac gtttcccgcc
     2821 gccggcctcc tgtgcgtcgt cttcttccac gtgcttcgac cgctttttct gtgagagctg
     2881 ccgtgcccga tttccccggc cctaccggtt tcctgacgcg ggtcgaaggg ccatctggca
     2941 gggttcgacc tgcgaccaag cgttgcctct ggaaggacgt atgatggcga ttggaacgtg
     3001 cttcccgctc ggaatgtatg aactaccccg caacgaggag gaatgggctc actggaaatc
     3061 cgccggaatc aatctgctgc cttgcgcgag ccgcgaggac ctggactcag tgcatcgcca
     3121 cggcatgaaa gggtgggttt cattgcccat ggtgctgcgt gaggacgacg acggcgcggc
     3181 gctgcgagcc gctgtcagcg ccctgaaaga ccatccggcg ctgtacgtat gggaggctcc
     3241 ggacgaaatc atctggaata ccaaccgcag ggttaccggt gtgttggcga gcttctggtg
     3301 ggatcagccg gaacggatgc gaaaagaact ggcgcaggaa tacgatgcgc tcgttctcgg
     3361 tctggaacgc ggctccgcga tcgtccgctc tctggacccg gggcgtccca tctggttgaa
     3421 cgaagccgct cgatccgaca tggagatcgt ttcccggtgt cttccgttcc tggatatcat
     3481 cagcactgac ttctaccctg ttcttcccca gactccagac ccccggcgcg cgaggatccg
     3541 cacacacgat atgatcgccc ttctttgcga ccgcttccgg gcgacagccc cggaccacga
     3601 gttctggttt gttcagcagg cgttttcgtg gcaccgcctt gagcggaaac gtcatcccga
     3661 cgtcccgatc gcgtacccta ccaggtgtga ttaccggttc atggcatggc aggcaatcgc
     3721 tcacggggcg cggggtcttc tctggtacgg ctctgcacat gaggaccggc ccgcgccgtt
     3781 cctggatgac ctcatggcgg tagtccgcga attgtccgaa ctctcgcctt ttctcctgtc
     3841 agaacacgca ccggttcttg aggttcgggt ggacacgaag cagtaccccc cgacgcgagg
     3901 tgtttcgtgc ttcatgcggg aattcggcgg gcggacattt ctcgcgctgg tgaacgaaga
     3961 cccttacgag aacgacgtac tggtgaccgg tgtgccgggc gaggagatta ccggttggac
     4021 ggcgttcggg gattcggaac gcccgcacgt gccgatagac aacggctttg tggtcccttt
     4081 gtcgggccgt gaggtccgcc tgatgatgag cgctgcctga gggaaccgcg tcaagtccaa
     4141 ccccgaagga gggagcttcg tgtttcctcg caagccccgc cgaatcgagg tttcaaaaaa
     4201 aactgtacca gaattgaaaa atcgaaggca aaagtattga cacgaacacg tttccttgcc
     4261 acagtcggaa atgacaggga agcgagaaga gtccgcgcaa gtgtgttgcg gtgctcaggc
     4321 gccggcgggt aattcgttgt cgggggcggg cggcgataac ctcggcgcgg ggaaatccgg
     4381 aatatccacc gtgcgcggga cgatatcgag ctgggggatt tctttgattt cgtttccggc
     4441 ggccgctttc agctccttga agcggcgggc ggaaaccaga acccgggttt cgaacgagcc
     4501 gatggctttg ttgtagctgt ccacggcgct tttgaggcgg tcacccaatc ggtcgaggtg
     4561 gtccgccatg ttggcgatcc gatcgtaaag ctcttttccc agcgcggcga tttcttccgc
     4621 acttttggcg atatcctcct gccgccatcc gtacgaaacc gcgcggagga gcgcgatcag
     4681 ggtgatgggt gaggccggga tgactctttg tttcacgccc tcttcgatga gagtgcggtc
     4741 ctgttcgaga gcgacagcaa aaagggattc ggcgggaagg aacaggacga caaactcggg
     4801 ggtgttgtcg aactggtccc agtagctttt tgcgctgagt tttttgatgt gttcacggac
     4861 gtgctgcgcc tgccgcgcga ggtgctcctt gcgtttctct tcgttgtctg tctcgatagc
     4921 atcaaggtag gcaaggatgg gggtttttgc atcgacgacg atgtttcgcc cgccggggag
     4981 ccggattatc atgtcggggc ggatttcgcc ttcttttgca gtcacctgct cgtcgaaatc
     5041 gcagtaattc aacattccgg ccatctcgac cacgcgacgt aactggattt cgccccatct
     5101 gccgcggaca ttgggcgttc gaagggcttg tacgagccgg gaggtctcgt ttctgagctg
     5161 ggactgctgg tccttcatcg cgttgacctg cgtcagtagc tcggcatacg cggatacacg
     5221 cttcgtttcc agttcctgaa gcttcgtatc catggagacg agggattcct tcaccggttt
     5281 gaccagttct tcaacggcct tctggcgcgc ttcgagatcc gtttttgcac cctcctggaa
     5341 tttctccaga tgggttttcg cgagttcgag aaaggccttg ttgttcgagt tcagcgcttc
     5401 cgaggaaagg gctttgaatg catcggaaag ttcggtccgt gcctgctgaa gcatggccat
     5461 tttctcgtcg gcggctttcc gttcctcggc gatacgggtt tccagttcgg aacggatttt
     5521 ttcgaggtcc gcattcgagg cccggagtgc ctcaagggat gattcgagtt cggggatacg
     5581 ggctgcctga gcctctgccg cagagcggcg ttcggcctcc tgctggagtt gctccgtgcg
     5641 gtggcgcagg tcatcctgaa gcacggcgag ctggccacga agactcgcag ccagctcgtc
     5701 ggttgagcgc aatcgttctt ccagcacggc gcgcgccgtc gccgcttcgg tggccgcctg
     5761 cgtccgggca cgttcgatac ggctccgcgt gacgagcaga gccgcggcga ccccgatcag
     5821 caggccgaca atgaaggcaa gcaacgcggg caacgcgagc atgggcgatt acggcctact
     5881 ggaagtggta cgtgacacca ataccgggaa gcccgggtcc ctgaatcagc gcgccccatt
     5941 tgccaaactg gagcccgatg ccgaaggagc cgataggacc gacccaggcg ccactgtgcg
     6001 tgccggatcc gtcatcgaac cgcgccgcca cgccaagccc gagctgaggg tagagcacca
     6061 tcgtcccttc gagcggaatg gcaaggcgga tcaggccttg aggtgacacg atgagggcgt
     6121 cgttgtaggt gcttgcgccg ccgaacccgc cgccggtcca gccgccctca actttccagc
     6181 cccagcgcaa ctgaggtgtg ccgccgccgg agaaaggctt tgtggtggca taccccactg
     6241 caaacgggcc gaagtacacg cttttgtccc atcccccgta tctcgtagcg gctgagacaa
     6301 acgaaggcgc gaggaatgag aagcccagca ccgccagcgc gacaatagcg cgtggtgaca
     6361 tagcgtacct ccaatattgt tcgagacttg cgtctcggtg tgacctcgtg ccatctttca
     6421 ccatggcttg aacgaacgcg gggaagacac cttgaaaaag ctgagtgtgg tcgctctcat
     6481 atgcggtctg ttcgcggcgt gttcccactc cgcaaactta agccgggcgc tgggttccgt
     6541 caatgagcgg cgggcgttcg ggtatctcac cgcccagact gcgttcggcc cgaggaaccc
     6601 tggaagccgg ggccacgcgg cctgccgtga ctacctcatc cgtcagttga acgcgctcac
     6661 cgatacggta gaattgcaac attttcagcc cattgactcg cgcggcaatc gcacgccgcc
     6721 gatgacgaac attattgggc ggattgcgcc ccaccagaac aggcgctatc ttttgtgtgc
     6781 acattgggat acgaggccgc gggcggatcg tgatcccgat ccgcgacggc gcgacctgcc
     6841 gattcctgga gccaatgacg gtgcttcagg ggtggccgtg cttctggaaa ttgccaccgt
     6901 tctcaaacga gtttcccccc ctgtgggcat agatattgtc tttttcgacg gcgaggacta
     6961 cggggaggaa ggacgcctgg aggactactt cctcgggtcg agggagtttg cgcggcgctg
     7021 gcgtggccag aagcacgaaa tggccattct ggtggacatg gtaggtgacc ggaatctcca
     7081 gttgcctgtc gaggcgttct cgtggcaagc agctccggaa gttgtcgaca gggtgtgggg
     7141 ggctgctgaa aggctgggcg aacgtgcgtt catccgctcc atgggcgtgg agattcagga
     7201 tgatcacgtt ccgctgattg aagccgggtg gcgggccatc gatgttatcg acttttccta
     7261 tccgtactgg catacccatg ccgacacccc cgacaagtgc tcgccacaga gcctcgggag
     7321 tgtcgcgcga gttcttctgg cggcgatcat cgaacggtag ccgtggctga caatggagat
     7381 ctccggctcc aatcaaagtc ccatgtgcgc gaacgaaagg cgctttgcct tctcgcgtcg
     7441 ggagcttgcc gccctcgccg cgttgacggt gagcctcgcg gtcggttgtt tccttgtctg
     7501 gctcattccg gagccatccg acctgcgagt cgttcgatcg cctgaactgg cgagtctggc
     7561 tcgcgaggtt gcgccgtcaa ccgggtccca tttgatcaac atcaacaccg cgtcgtttga
     7621 cgaactggtt tccctccccg ggatcgggcc gaagaaggcg caggcaatac tggattatcg
     7681 gcatgcgaac ggacgctttc ctgatgtggg tgcgattgag agggtgaaag gcattgggcc
     7741 tgcgacggtg gcacgccttc ggccgctcat cactgttcag gaccgggcac ccgcgggtga
     7801 acgcagttcc ccggcaacgg ggggatcgag atgagacgca aagccatgcc ggtggcggtc
     7861 cggctggtga tagctgtgat agcgttcagc gcggctgcca tcccgcccct catcagatac
     7921 agcgcggcgg aagaggcgga ggaatgcgcg cgtcggcggg aggcagtaag aaccgcggca
     7981 tcgcggttgc cggacgaacg ctggtggaac gaacgcgggt gcccgcgtga tggcgagaac
     8041 taccgctttg ccgtcgggca ggacagcacg gtggaagtag tgtgtcccaa agggcatggg
     8101 attcagaagc ttcccgtgca ccgatggttt tccccgttct gaacgacggt tgaaaggacg
     8161 atccgacatg cgtcgcgcgc tctatccggg gaccttcgat cccatcacgc tggggcatct
     8221 cgacatcatt gagcgaagcc tgcggatttt cgacgaagta gtggtatctg ttgcggacac
     8281 ccggcccggc gtggtgtttc ccgtggaaga tcgtgtcagg ctggtagaag cagcgacgaa
     8341 gcatctcgat cgagttcgcg tggtctcgtt ccgcgcgctc ctcgttcacc actggcaaag
     8401 cctcggcaag ccgacagtgg ttcgcggatt gcgcgcagtg caggatttcg aagcagagtt
     8461 cgcaatggcc ctcggcaaca gaaggctcgc cgatgatttc gaaatcatat ttttgatgcc
     8521 gagaaatgac ttcgtgtttc tcagcgcgtc gatagtcaga gaggttgcgc ggtggggtgg
     8581 ggacgtctcc agcatggttt ctccccctgt cgcggagtac ctcgcaacca gatacggccc
     8641 gccggcgaag tgacaattgc ccggctgtgg ccttggacat catatgtggg gaggcaaccc
     8701 tgatgagcag taccgtcatt caacaaatcc gcgaacgtgc agccgcacac aagggacgta
     8761 tcatcctgtc cgaggggaac gaacttcgag ccattcaggc ggcgcggaaa ctcctcgacg
     8821 acgggatctg cgaggtgaca ttggtgggtg cgccggcgga gatcgggaaa gtggcaaaag
     8881 aggggggtgt ccgggtatcc ggcatcacga tggtggatcc gtcggaacac cccatgcggg
     8941 agcaatttgc cgcggagctg tatgaaaagc ggaaggccaa gggcctgaca aaggaacaag
     9001 cggaggaact ctcacggcgg cagatttact gcgccgcgat gatggtgagc cacggcgagg
     9061 gcacgggtac cgttggcggc gcggtgcacg cgaccgcgga aatcgtccgc gccgggctct
     9121 ggtgcatcgg gatggcgaag gatgtgagcg tcgtctcggg tgcgttcctc atggtagtgc
     9181 ccgatttcct cggaacgggc aaggacaaaa cgctgtatta cgccgattcc ggcgtagtgc
     9241 ctgacccgaa cagcgaccag ctcgcttcca ttgccattgc cagcgccgcc acgttccgtg
     9301 cgcttaccgg tgaggagccg cgcattgcaa tgctgagctt ctccaccaag gggtcggcga
     9361 cgcaccccga cgttgacaag gtgattgttg cgacgaagaa ggttcaggaa ttgcggccgg
     9421 acctcctcat tgacggcgaa ctgcaggctg acgcggcgct cgtcccgggt gtggcgaaag
     9481 cgaaggcgcc gggcagttcg gtcgccggaa ctgcgaacgt cctcattttc ccggacctcg
     9541 gcgcgggaaa cattgggtac aaactcaccc agcggcttgc ccacgccacg gccctcgggc
     9601 cgatccttca ggggttggcg aagcccgcgc acgatctttc ccgcggttgc agcgccgagg
     9661 acatcgtgga tatggcggcg atcgcgatct gcatggccga atagctccgc agccgagcca
     9721 ccacgaaaaa cggggaagcg ccgaaacgcg cctccccgtt cgatttccgc ctcaggcagg
     9781 gctcagatcc atttgtcttt cggattgtcg agttgcccgg caatgacgct ttctcggaac
     9841 tcctccgtga tgtgggcgac gtagacctgc gccattcccg ttctgtcgga ggtgaacacc
     9901 ccgattctgc cgtcctgact cagcccgggg tgcggatgcc cccactgagc atggccctga
     9961 gtggccccgg catggcagag tgtgcggaaa tgccggctgg ggacgtgcac gagctggagc
    10021 ccctgatccg gccagttcgt gtcggagagg atccattcgg cgtcgtacga ggctcccgcg
    10081 tgccagaaat aggggccttc cacgagcttg tacggttcgg tgcccgcttc ggcaatccag
    10141 atgcaacgat gaggaggctg gctgggcccc tggattttgc tcgtgttgcc gagaatcgtt
    10201 gagtgggcga acatcccgac gtcacagatg tactgctcgt cgctgccgtc ctcgttcacg
    10261 ctgtagtacc gcatcggctc cgggttgaca ttgtggaaca cacggtttcc gttctcggtg
    10321 atcctcgtgc cgagtttcat gaagttcttc gtgacgatga gcctgccggt ggcctgatct
    10381 accgccgcga ttgtcatgtt cgtgtcattt gccgtgacgg cgcccgtggc aaggtctatc
    10441 cgtatcatcc tgccttcccc tgccgcgtaa tcccgcaggt cgaggtagag gcgggaaccg
    10501 actgatccgt accggacgcc gcagacgatc atgccgggcg cgcagtggtt gccgacggcg
    10561 atgaggtctt ccatcgctcc ggtttcgata tcgagttttc gcaggatttt ctcgtcgcgc
    10621 cagagcacgt atgcgctctt gccgtcgagg ctgagcgtca cattgggata ccccgcggtg
    10681 tcttctttcc gtgtgagctg acgggggtcc aacccatcgg tgtcgcaccg atagatatcc
    10741 cacggcgcct cgcgcgacgc ggcgcgttga acaaggaata cgacgcgcct gttgtccggc
    10801 gtgacgctgg gccagtcgta catgagcgga atgctgggtg tgggataact catcaactgg
    10861 acaacttgaa ttccggtttt cgggtcgaac atctgaacac gttcactcat cgagagactc
    10921 cttcgtgtgt gcggacctgc gccattccct ttcggggcag caggcgtttc gcgcgtgcgc
    10981 tcatatccat ttgtctttcg ggttgtcaag ttgccctgcg acaacgctct cgcgaaactc
    11041 gtctgttatc agggcgacat agacctgtgt tatcccggtc cggtcggagg cgaacactcc
    11101 gatacggcca tcctggctga gaccgggatg cgagtgcccc cactgagaat gtcccagcgt
    11161 cgcaccagaa taacagaccg tgcggaagtt ccggctgggg acgtgcacga gctggagccc
    11221 ctgatccggc cagttcgtgt cggagaggat ccattcggcg tcgtaggagg ctcctgcgtg
    11281 ccagaaatag ggtccctcga caagtttgta tggcaccttg ccggcttcgg aaatccagat
    11341 gcaacggtgc ggtggctgac tcgggccttg gatctgactc gttttgccga gaatcgtaga
    11401 gtgcgcgtac atatcgacgt cggcgatgta ttgctcgtcc gttccgtctt ctttgacgct
    11461 gtagtatctc atgggctccg ggttggcgtt gtgaaacacc cgtgctccgt ccgcgcccat
    11521 cttcgtgccg agtttcatga agttctttat gacgatgagc cggccggtgg cctgatccac
    11581 cgccgcaatg gtcatattcg tgtcgtttgc cgtgaccgca cccgtggcaa ggtccacgcg
    11641 tagcatcctg ccttcgccgg ccatgcaatc acgcaagtcc aggtacaacc gcgaacccag
    11701 cgaaccgtat cgtacggcga gtaccgccag tccgggcggg cagtggtcgg cgacggaaag
    11761 gaggtcctcc atcgcgccgg tttccatgtc catcttccgg agtatcttct cttcccgcca
    11821 gatgacgtac gcgctctttc catcgaggct gagggtgacg tccgggtacc ctgcgctcgc
    11881 gtccttcttc gtgagccgga caaggtccag accgtccgta tcacaacggt agatgtccca
    11941 cggcgcttcg cgctccgcac tgcgctgggc aaggaacacg acacggcggt tatccggtgt
    12001 gacgctcggc cagtcataat tgagcggtat gctgggcgtg gggtaactcg taagctggat
    12061 gacctgtatc ccggttttcg ggtcggtcat ctgcacgcgc tcactttgca tggtatcccc
    12121 tcgggaatcg gtttggtgcc tttttcgcgg gaaacgcacg tatgaattac gcaccgcagt
    12181 gaggaagtgg caacgcaccc ggtttggcgt ttccctatct tttgtacgtc gcaaccggcg
    12241 caatccggcg ttctctgagg ctttttcacc atctgaacag gaacaaaaaa tggcacaatt
    12301 cgtcacgttc gggctcccca agggcagcct tcaggaagca acgctcgacc tcatgaaacg
    12361 tgcggggttt gagtttaccg taagcagccg ctcgtatttc cccacgtgtg acgacccgga
    12421 agtgaaggcc gtcatgttcc gcgcccagga gattgcgaaa tacacggagc gcggcgtcat
    12481 ggatgtcggg ctgacgggcc tcgactggat actcgacagc ggcgcggacg tggtggaggt
    12541 ggcggacctg atttacagca aagtcacgac gcagaaggcg cgctgggtgc tggcggtgcc
    12601 ggagaactcg cccatcaagg aggcgaaaga ccttcagggc aagcgcatcg cgacggagct
    12661 ggtgcacgtg gtggaggcgt acctgaaacg gcacggcgtg aaagcggagg tcgagtactc
    12721 ctggggcgcg actgaggtga
//
LOCUS       DAOSOO010000078        12698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4943_length_12698_cov_5.195286, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000078 DAOSOO010000000
VERSION     DAOSOO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_12698_cov_5.195286"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLT86_07280"
     CDS             <1..904
                     /locus_tag="PLT86_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT36449.1"
                     /translation="RPWCWRRQRGRWRTRKMIVSVASGKGGTGKTTIAVALAQVAPHS
                     AYVDCDTEAPNGHLFLRPNISHRKPVSRTVASINVNTCTWCGECMEKCAYHAIVIAHA
                     TRKAVVFEDLCMGCGVCAFVCPARAITEEERHAGIVSNGHSGRIRTREGRMAVEAPHA
                     HPVLTETLKELPDAPCTILDAEPGVTGRVVTTVRASDVCVLVTEPTPFGLHDLELAVT
                     MTQHVGVPVGVIINRSTIGDNAVRDFCRVRNIPVLLEIPFDRRIAAGYAVGKTLVEIL
                     PDMKSRFAEVLRHVERIVTERGRS"
     gene            901..1809
                     /locus_tag="PLT86_07285"
     CDS             901..1809
                     /locus_tag="PLT86_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT36450.1"
                     /translation="MTPQITVVSGKGGTGKTTLTALWGSMADRPVLVDADVDASNLPL
                     AYPHETREQHPFVGRDVAVVNEPLCKNCGVCARACRFDAFFQVEGQFRVDPLACEGCT
                     LCTLMCPLDAITMKPHVSGTWSVSETGFGPLVHAQLGIAEGNSGKLVTQVRRAGEKLA
                     EERAAAVILVDGPPGIGCQTTAAMAGASLVVLVTEPSASGRHDMERLLSVTNRLGVVA
                     LVVLNKVDLAPAEEALIRTAVSRMGAEYIGSLPFIQEVPGLLASGTLISECPRALREA
                     AEGLWKVILERALVAERVQQGEPAHG"
     gene            1836..2021
                     /locus_tag="PLT86_07290"
     CDS             1836..2021
                     /locus_tag="PLT86_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36451.1"
                     /translation="MPLGDGKGPPEGTGPMTGRRRLAASCPSKKPEKASGWSLLGLVV
                     VALLAIGLWFVSDNTDD"
     gene            2368..2694
                     /locus_tag="PLT86_07295"
     CDS             2368..2694
                     /locus_tag="PLT86_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT36452.1"
                     /translation="MPAGDGTGPLGMGPMTGRRAGICAGFGVPGYANPGAGFWCGGGR
                     GRGFRNRYWATGIPGWAWWPGVSGTVPAPVTEEAEAEALRQQAAALQRSLDAINQRLA
                     NLTSEK"
     gene            complement(2787..3917)
                     /locus_tag="PLT86_07300"
     CDS             complement(2787..3917)
                     /locus_tag="PLT86_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36453.1"
                     /translation="MYRRPDYATVRNPKEFSWSVSGSYLSCLALSGVPLRDFFLKPDA
                     CIEAYRVGREKQRQLFDPQDVSLPSPATPPVSYGHANGLGARLFFPEGGEVAVEHCFA
                     HSLEEAMEALARPVDFASAGMAPFYLDFREQMMRAFPGESVGFSFGAEGPCTTAYELR
                     GDAFFVDVMEEPARTKEFLSRINDSILAFEQFRLGVSGGKVFNESGASLCDDIAAMIP
                     PRLWNEFVLPFWSDLFCRRTTGDVHAHVEDLKPAHLPFLEQIGLSYYDPSISHHLNPR
                     IIADKCRVPFGWRLGGIHLPVLDAQDVEDFVFQSAADGASRVFTILEANSCSGENIPK
                     IDAFIRAAKEVKRLLDEGCPRRALADHVSERGRHKFWDNWCS"
     gene            4164..7517
                     /locus_tag="PLT86_07305"
     CDS             4164..7517
                     /locus_tag="PLT86_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT36454.1"
                     /translation="MSCPQLPTRVKNVLVALAAAFSFSPAFAAGIEQIWVADAHMVME
                     GAPMVVDLDGDGDAEILTAAYEHIIVMDGSGSELWRYDGRGRYSTCPAILERRGDTPL
                     IFAGDNTGEFTCLDGRGNVVWKRDLPSIFCASPAVADLDANGTFEVVQGDKTGKIHAM
                     DALSGALLWTAQLDGECASPAVGDLNSDGKLEICITTGAGKVYAVSATGVVLWSVDLG
                     SPSPDWSTCSPVIFTGSDGNQYVAAGARSGRFVCLNASGEVVWERDLRGAIASTISVS
                     DFDSDGIADVFVVTQLGVLYRFDETGRVIWDIDTQGRSLAAGAIVDVDGDFALEYVLC
                     TQNGNLMVCNQTGEITFNHQFPNRTINVTAAFGDIVKTRPGLEFAITGGESGKVYCFG
                     TAAPVNTAAPWRTYRRDNELTGCWSGLRSDTGARMTPANLAWDRILTGEDVVFHIFLP
                     GPVGLAKAEATCIRPDGSRQSSIGAVAGQRGVLQMPVVITAPGVYRFEWNVKDGAGRR
                     LAGGSRELTLHPYQNDRAFVRHTLGELRSALGTAPVPRSERGIRAALQKEMRDIEREA
                     DALVELQDATPGAARELVEEANARTIALNERARRASALARVARALPPGASSPRVVAFE
                     GTTWENRDINRQLPAAVAIPLTISRRAVTGEHEPVSIKLLNVTLDTVRAEVRVETSNA
                     GIRVTPMEAKAVPTNLNTKGWDALPGLGNRAIAVPPLETGEVWLDIDLANTPPGKHTV
                     TAFFGRGSAAARAIITLDVLPFEMAGYRDLRLCNWAVYDGDAVPDLLAHGNTVFITGM
                     PHATISPENPDRVSVDFAPLDAFVEPLRGHDAFLLLSGVPNLGVPVSSDTFVVRFGDY
                     IRQVLGRLAQRGIPERNVALYPYDEPGGSGWNTVNRYIEFARQALKAYPRLLFYVNGG
                     GDLPMFQAFNEVAGIWCPGYYMLPDSSAEMNFIRASGKTLWSYDCGIGYARPIGWHTK
                     TINVAGQYRMAPVFTVAFGATGLGYWCYNHGPSMWEAVEAEFPLVYVNPDTTHTASRR
                     WEAVREGMEDARIMLALREKLSDEHVNAAAKQKIRHLLEVTAPDFARHSLEEVRLGVA
                     RYALDATNNDDTVGAFRQELLDCVAATVE"
     gene            7651..10101
                     /locus_tag="PLT86_07310"
     CDS             7651..10101
                     /locus_tag="PLT86_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT36455.1"
                     /translation="MAQGSTTRTTLPISGMSCAACAARIERKLRALDGVADAEVNLAA
                     HTALVTHDPGTAPLPQIVDAVREAGYDVPVETLRLPVEGMSCASCVSRVERAVKGVDG
                     VLDAQVNLATGEATVRAVLRDDSVTRAIAAAVEKAGYGVRSVEYSRQPSADPVARSEE
                     EFRNLRRRFAIAAVFSIPVFLISMGLVPGMARLPFQTTAILLLALTLPVQFWAGWQFH
                     RGAWQQLRHASADMNTLISVGTSAAFLFSLVLTFHPAAFRSTGVHHAVYYETSAVIIT
                     LVLFGRMLEARAKARTTQAMSALLALRPVAAHVERDGQIRDVDPADVVAGDVLVVRPG
                     ERLPVDGVVLDGFSSVDESMLTGESFPVEKKQDDTVTGGTLNVAGSFRYRATRVGNET
                     TLAQIIRLVEHAQGSKAPVQRLVDRISAVFVPTVIAISAVTSVVWWAFGPEPALHFAV
                     LNAVAVLIVTCPCALGLATPTAIMVGTGRGAEMGIFIRSGETLERVGRLTTVVFDKTG
                     TLTQGEPVVTGVHTVGTLPADDVLVLAASAEARSEHPLGAAVVRKAAERSLPLLSCEE
                     FSAESGHGLRAMVANRVVLIGNSALMQAQGVRLDAGRSVAEEIQAKGATAVFVALDGA
                     LEAVFEIADTPKPHAAEVIAALKRDGLKVVILTGDSRRTAEAIASQLGADDVIAEVKP
                     GAKADYVAALQKSGEVVAMVGDGVNDAPALALADIGIALGTGSDAAMETAGITLMGGD
                     IRNVRKAIALSRATLRTIRQNLFWAFIYNVAGIPIAAGVLYPVNGMLLDPMLASAAMA
                     MSSVSVVTNSLRLRRWNG"
     gene            complement(10162..10935)
                     /locus_tag="PLT86_07315"
     CDS             complement(10162..10935)
                     /locus_tag="PLT86_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOT36456.1"
                     /translation="MIGALAGDIIGSVYEWDNIKTKDFPLFSPRCTFTDDTVLTVALA
                     DSILSGVPFARNMKTFYRLYPDRGYGSSFHTWARSTDMSPYQSWGNGAAMRISPVGYA
                     YDDLEQVLRKAKEFTEVTHNHPEGIKGAQATAASILLARKGKTKDEIRSFVESEFGYD
                     LSAHVDEIRPTYTFDESSQGTVPQAIRAWFDSTDFEDAIRTAVSLGGDSDTVACITGG
                     IAQAGYGGVPEHISARVYETLDERLRAITQQFMMRFCGG"
     gene            complement(10932..11666)
                     /locus_tag="PLT86_07320"
     CDS             complement(10932..11666)
                     /locus_tag="PLT86_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36457.1"
                     /translation="MSLTAAFASILMSATRAHAQYTVRAGAATGGTVGEWGRVAGFLF
                     GVDQMTPSRSFGWWSTHFNVERLSTRFERTEKLAGLRLIRTYTSPPETTAFKNSFSVT
                     AITLGARYEPCRRWLGFRQTSKHLLSPFVGWYGGYMRMEHTGSREYHVAWTSVSSHGV
                     PVGAVAGYFMGWGGPPENALDPGVSSTYTGLEIGLDVSSTAWISGHTFVAPRAGLPDG
                     LKTPYAPRVLLRVSIAHRTAPVRRDG"
     gene            complement(11696..12325)
                     /locus_tag="PLT86_07325"
     CDS             complement(11696..12325)
                     /locus_tag="PLT86_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOT36458.1"
                     /translation="MAFSSPQDGVTAELHPWRTIWTHPRQTVAAMTVYAPWRWLWLLI
                     LAGGTSRIVERIALLPSLVELEPEALTLLILVSGALAGALMVFVIARILHWVLRRIGG
                     VGSWTATRTAVAWALAPGIPSLALWAIMVTGYGYSALTPSLISGSDDPRALVLTVDYG
                     VQFGLTVWSLLIEILCLSDAHRLAVWKVIAAEAGLGLAIGSLTLIGFNL"
BASE COUNT         2441 a         3603 c         4283 g         2371 t
ORIGIN      
        1 gaggccgtgg tgctggcggc ggcagagggg ccggtggcgg acgcggaaga tgatagtaag
       61 cgtagcaagc gggaaaggcg gaaccggcaa gaccacgatt gcggttgcgc tggcgcaagt
      121 ggcgccccac agcgcgtatg ttgactgcga caccgaggcg ccgaacgggc atctgtttct
      181 gcggccgaac atttcacacc ggaaaccggt gagccgtacg gtagcctcga tcaacgtgaa
      241 cacgtgcacc tggtgcggtg aatgcatgga gaagtgcgct taccacgcca tcgtaatcgc
      301 tcacgcgacg agaaaagctg ttgtatttga ggacctgtgc atggggtgcg gcgtgtgtgc
      361 gtttgtgtgc ccggcgcgcg cgatcaccga agaagaacgg catgcgggca tcgtctcgaa
      421 cggccacagc gggcggattc gtacgcggga gggccgcatg gcagtggaag ccccccacgc
      481 acatccggtg ctgaccgaga cattgaagga gctacctgac gcgccgtgca ccattctcga
      541 cgcggaaccg ggcgttacag gtcgggtcgt cacgacggtc agagcaagcg acgtgtgcgt
      601 ccttgtcacc gagcccacac cgttcgggct gcacgatctg gagctggccg tgacgatgac
      661 gcagcatgtc ggggttccgg tcggcgtcat catcaatcgt tcaacgatag gcgacaacgc
      721 cgtacgcgac ttttgccggg ttcggaacat acccgttttg ctggagattc cgtttgatcg
      781 acgcattgca gcgggatacg cggtgggaaa aacgcttgtt gagattctgc cggacatgaa
      841 aagccgattt gccgaggttc tcaggcatgt cgagcggatc gtcactgaga gggggcgatc
      901 atgactccgc agattacggt ggtaagcggg aaaggcggaa ctggaaagac gacgcttact
      961 gctctttggg gcagcatggc agacaggccg gtgctcgtgg atgctgatgt ggacgcgtcg
     1021 aaccttcccc tggcgtaccc ccacgagacc cgggagcagc atccgttcgt cggacgggat
     1081 gtggccgtgg tgaacgagcc gttgtgcaag aactgcggtg tgtgcgcgag ggcgtgcagg
     1141 tttgatgcgt tttttcaggt tgaaggccag ttccgggtgg acccgcttgc gtgcgaagga
     1201 tgcacgctgt gcacgctgat gtgcccgctc gacgcgataa ccatgaaacc gcacgtctcc
     1261 gggacgtggt ctgtgagcga aaccgggttc ggacctctcg tccatgctca actgggaatt
     1321 gctgagggga attccggcaa actggtgacg caggtgcgcc gggcggggga gaagctggca
     1381 gaggagcggg cggcggcagt gattctggtt gacggccctc ccgggattgg atgccagacg
     1441 acggcggcga tggcgggagc atctcttgtg gtgctggtaa ctgaaccgtc ggcgtcaggg
     1501 cggcacgaca tggagcggct gctttcggta acgaatcggc tgggtgttgt ggcgcttgtc
     1561 gttcttaaca aggttgacct tgcacccgct gaagaggcgc tgattcggac cgcggtatcg
     1621 cgcatgggcg cggagtatat agggtctctt ccgttcattc aggaggttcc cggcctgctt
     1681 gcgtcgggaa ccctgatcag cgagtgcccg cgcgcgctga gggaagcggc ggaaggactg
     1741 tggaaggtga ttctggaacg cgcgctggtg gcggagcgcg ttcagcaggg agaacctgcg
     1801 cacggataac gcaccggaaa ggatgggcga agaaaatgcc tctgggtgac ggaaagggac
     1861 ccccggaagg gactggcccg atgacggggc gcaggcgcct cgcggcaagc tgcccatcga
     1921 agaagcctga aaaagcgagc gggtggtctc ttctcggact tgtcgtggta gcgttgcttg
     1981 cgattgggct gtggtttgtc tcagacaaca cagacgattg agattgcacc acgaaaagga
     2041 gatccacatg ccaggagtac ggggaatgag accgatggga aggcggtgtg ctgcgcttgc
     2101 ttccggttgg ggtggatacg cggtgcggcc gacctccttt gcggcgggat atcgcttcat
     2161 tcgttgcggc cgaggacgcg gcgggcagtg gtgccgcgag cgggggtatc ctcaggtatt
     2221 tttcgcggcc ggatctgcgt ggagacagcc cctcgtggcg cctgtgggtc agcggtgggg
     2281 gttttgattc aaaatcgcgc tccagatgat ggtcagcatc atgagggcgc acgtaaatca
     2341 acacacttac gataaggagg cacagcaatg ccggcaggag atggcactgg tcccctggga
     2401 atgggaccga tgacgggtcg gcgcgcggga atttgcgccg gattcggcgt tccgggatac
     2461 gcgaaccctg gagcgggttt ctggtgcggc ggtggccgcg ggcggggatt ccgcaatcgg
     2521 tactgggcaa caggaatacc cggatgggcg tggtggccgg gcgtatcggg aaccgtccct
     2581 gcgcccgtga ccgaagaggc cgaagcggaa gcactgcgtc agcaggcagc agctcttcag
     2641 aggtcgctgg atgcgatcaa ccagaggctt gcgaacctga cgtcagagaa gtaaactgcc
     2701 gataccgtcc gcccctgtgg agccgggatg ctctcaaggg gcagagcagc gggggagtcc
     2761 acgaggactc tcccgctttt cgtgtgtcag gagcaccagt tatcccagaa tttgtgtctg
     2821 ccgcgttcgc tgacgtgatc ggcgagggct cttcgcgggc atccctcgtc gaggagacgc
     2881 ttcacttctt tcgccgcgcg gatgaacgcg tcgattttcg gtatgttttc cccgctgcac
     2941 gaattggctt ccagaatcgt gaacactctg gaggccccgt ccgcggccga ttgaaagaca
     3001 aaatcctcca catcctgcgc gtccagaacc ggtagatgaa ttcccccgag gcgccagccg
     3061 aatggtacgc ggcacttatc cgcgatgata cgggggttca ggtggtggga gatggacggg
     3121 tcgtagtacg acagcccgat ctgctccagg aaaggcaggt gagcgggttt caggtcttcc
     3181 acgtgcgcat ggacatcacc cgtcgttcga cggcagaaga gatcgctcca gaacggcagg
     3241 acgaattcgt tccagagccg ggggggaatc atggcagcga tgtcatcgca caggcttgcg
     3301 ccgctctcgt tgaacacctt cccgccggag acgccgaggc gaaattgctc gaacgcgagg
     3361 atgctgtcgt tgatccggct caagaactcc ttcgtccgcg ccggttcctc catcacgtcc
     3421 acgaaaaatg catcaccccg caattcgtag gctgtcgtgc agggaccttc cgccccgaaa
     3481 gaaaagccga ccgactcacc gggaaacgct cgcatcatct gttcgcggaa gtccaggtag
     3541 aacggggcca tccctgcgct ggcgaaatca accggccgcg cgagagcttc catcgcttct
     3601 tccagcgagt gcgcgaagca gtgctcgacc gcgacctcgc ctccttcggg gaaaaacaac
     3661 cgggcgccaa ggccgttcgc gtggccgtaa cttaccggcg gcgtggcggg agacggcagg
     3721 gaaacatcct gcgggtcgaa aagctgcctc tgtttttcgc gcccgacccg atacgcctcg
     3781 atgcaggcat cgggtttgag aaagaaatcg cggagaggga ccccggacag cgcaaggcag
     3841 gagagataac tgcctgacac gctccatgag aactccttcg gattgcgcac ggttgcgtag
     3901 tcgggtctgc ggtacataat ggcctcctga taccaatgaa tcgggaaaag tggccacggg
     3961 agcaagctgg cacctgaacg ggagttcacg agggaacttg gtgcggaggc cgcccggcac
     4021 tattgtacct agcgagtccg ggagtctggc tgtcccggcg tcagtgattg tgttcccaca
     4081 ggttgcatcc tcctgaacgc cctcatgcag ggcggcgagc gtcttcctca aggttttttg
     4141 actccgcgga aaggaatctc agcatgagtt gcccacaact acccacacgg gtaaaaaacg
     4201 ttctggtggc tctggcagcc gctttttctt tttcgccggc tttcgcggcc ggtatcgagc
     4261 agatctgggt ggccgacgcc cacatggtga tggaaggggc gccaatggtg gttgacctgg
     4321 acggagatgg cgacgcggaa atcctgaccg cggcgtacga acacattatc gtaatggatg
     4381 gttcgggctc ggagctctgg cggtacgacg ggcggggacg atattcgacg tgtcccgcaa
     4441 ttctcgagcg ccggggagac accccgctca tcttcgccgg ggacaacacg ggtgaattca
     4501 cgtgcctcga cgggcggggc aacgtggtgt ggaagaggga tctgccttcg atcttctgcg
     4561 cgtcgccagc cgtggcggat ctggatgcga acggcacgtt cgaggtggtt cagggcgaca
     4621 aaaccggcaa aatccacgcg atggatgccc tgtcgggagc tcttctgtgg acggcacaac
     4681 ttgacgggga gtgtgccagc ccggcggtcg gcgatctgaa ttccgacggg aaactggaaa
     4741 tatgcatcac caccggagcc gggaaggtat acgcggtgag cgccaccggt gttgtgctct
     4801 ggagcgttga tcttggttcg ccctcgccgg actggtcaac ctgttccccg gtgattttca
     4861 cggggtccga cgggaaccag tacgtggcgg ccggtgcgcg ctcggggcgc tttgtctgcc
     4921 tcaacgcgag tggtgaggtg gtctgggagc gcgacctgcg gggcgccatc gcatcgacaa
     4981 tttcggtgag cgacttcgat tcggacggga tcgccgacgt ctttgtcgtc actcagcttg
     5041 gcgtgctgta ccgttttgac gaaacgggca gagtgatctg ggatattgac acgcagggga
     5101 gaagccttgc tgccggagcg attgttgacg tggacgggga ttttgccctt gagtacgtgc
     5161 tgtgcactca gaacggaaat ctgatggtgt gcaaccagac cggagagatc acgttcaacc
     5221 atcagtttcc gaaccgcacc atcaatgtta cggcggcgtt cggcgacatc gtgaaaaccc
     5281 ggcccggtct ggaattcgcc atcaccggcg gcgaatcggg aaaggtgtac tgctttggca
     5341 cggccgcgcc ggtgaatacc gccgcaccgt ggcgcacgta ccggagggac aacgagctga
     5401 cgggctgctg gtccggcctc aggtccgata ccggggcacg catgacgccg gccaatctcg
     5461 catgggaccg gatcctgacc ggagaggatg tcgtgttcca cattttcctg cccggtcctg
     5521 tgggccttgc gaaggcggag gcaacatgca tcaggccgga cgggtctcgc cagtcttcga
     5581 taggcgcggt agccggccaa cgaggcgtgc ttcaaatgcc ggtggtgatt actgcaccgg
     5641 gggtgtatcg gtttgagtgg aacgtgaagg atggcgctgg aagacggctc gccggtggct
     5701 cgcgggagct gacgttgcac ccttatcaga acgatcgcgc gtttgttcgc cacacgctgg
     5761 gcgagctgcg ttccgcactg ggaactgcgc cggtaccgcg atcggaacga ggtatccgcg
     5821 cggcgttaca gaaagagatg cgggatattg aacgcgaggc ggatgcgctg gttgagcttc
     5881 aggatgcaac ccccggagcg gcacgggagc tcgtggagga ggcaaacgcg cggacgattg
     5941 cactgaacga gcgagccaga cgagcttcag cacttgcgcg ggtcgcgcgt gcactgccgc
     6001 cgggtgcctc ctcgccgcgg gtggtcgcgt ttgaagggac cacgtgggag aaccgggaca
     6061 tcaaccggca gctaccggcg gcagttgcga tcccgttgac gatttcccgt cgggcggtaa
     6121 ccggggaaca tgaaccggtg tctatcaagc tgctcaatgt aacactcgac acggtgcggg
     6181 ctgaggtgcg cgtggagacg agcaatgccg gcattcgagt taccccgatg gaagcgaagg
     6241 cggttcccac gaacctgaac acgaaaggct gggatgccct gcccggactc ggaaatcgcg
     6301 caatcgcagt tccgcctctg gagacaggtg aagtgtggct ggatattgat ctggccaaca
     6361 cgccgccggg caagcacacg gtaacagcgt ttttcggaag aggcagcgcg gccgcccgcg
     6421 ccatcatcac gctggatgtg cttccgtttg agatggcggg gtaccgcgat ctacgcctgt
     6481 gcaactgggc ggtatacgat ggcgatgcgg ttccggacct gcttgctcac gggaataccg
     6541 tattcatcac ggggatgcct cacgccacca tcagccccga gaacccggac cgcgtatccg
     6601 ttgatttcgc cccgctcgat gcgttcgttg agccactacg ggggcacgac gcgttcctgc
     6661 tgctgagcgg cgtcccgaac ctcggtgttc cggtgagcag cgacacgttt gttgttcgat
     6721 ttggcgacta catcagacag gtgctcggtc gccttgcaca gcgcgggata ccggagagga
     6781 atgtggcgct gtacccctac gacgagcccg gaggcagcgg ctggaacacg gtcaaccgct
     6841 acatcgagtt cgcgcggcag gccctgaagg cgtatccgcg gcttctgttc tacgtgaacg
     6901 gcgggggcga tcttcccatg ttccaggcat tcaacgaagt ggcgggaatc tggtgccccg
     6961 gctactacat gctgccggac agctccgccg agatgaactt cattcgcgcg agcgggaaaa
     7021 cgctgtggtc gtacgactgc ggaatcggat acgcgcggcc aatcggctgg cacaccaaga
     7081 cgatcaacgt tgccggacag tatcgcatgg cgccggtgtt caccgttgcg ttcggcgcca
     7141 cggggctggg gtactggtgt tacaaccacg ggccttccat gtgggaggcg gtggaagcgg
     7201 agtttccgct ggtgtacgtc aaccctgaca ccactcacac tgcttcgcgg aggtgggaag
     7261 cagttcgcga agggatggag gatgcccgca tcatgttggc gctgcgggaa aagctctccg
     7321 atgagcacgt gaatgctgcg gcgaagcaga aaatccgcca tttgctcgaa gtgacggcac
     7381 cggattttgc ccgccactca ctggaagagg ttcggctggg cgtggcgcga tacgctctcg
     7441 atgcaacgaa caatgacgac acggtgggcg catttcggca ggaactgctg gactgcgttg
     7501 ccgcaacagt cgagtaagcc gcaccggttc ggggcgcaag aggccggcag gggcgtttcg
     7561 cctcctgccg gtttcctttt gttctgcgcg gagtacgttc gtccttctcc tgtgttgttc
     7621 ttcagtgaac cagtgtgaaa gggaaccgta atggcacagg gatccaccac ccggactaca
     7681 ctaccgattt cgggcatgag ttgcgctgcg tgcgcggcgc gcattgaacg gaagttgcga
     7741 gcgttggacg gcgttgcgga cgcggaggtg aatctcgcag cgcacacggc gttggtcacc
     7801 cacgaccccg gtacggcacc gttgccgcag atcgttgacg ccgttcgcga ggcggggtac
     7861 gacgtgccgg tggagacatt gcgcctcccc gtggagggga tgagctgcgc gtcgtgcgtg
     7921 tcccgcgtgg agcgcgcagt taagggcgta gacggcgtgc tggacgccca ggtgaacctt
     7981 gcgacaggcg aggccaccgt ccgtgccgtg ttgagggatg attctgttac ccgcgcgatt
     8041 gccgcggcgg tggaaaaggc cgggtacggc gtcaggtcgg tggagtattc gcgtcagccg
     8101 tccgcagatc ccgtggcgcg ttccgaggag gaattcagaa acctgagacg acggtttgcg
     8161 attgccgcgg tgttttcgat ccccgtgttt ctgatcagca tgggcctggt gccgggtatg
     8221 gcgcgtctgc ccttccagac caccgcaatt cttctcctcg cgctcacctt gccggttcaa
     8281 ttctgggcgg gatggcagtt ccaccggggc gcgtggcagc agttgcgcca tgcgtccgcc
     8341 gacatgaaca cgctgatttc ggtggggaca agcgcagcgt tccttttcag cctggttctg
     8401 acgttccatc cggccgcgtt ccggagcacc ggggtacacc acgcggtcta ttacgaaacg
     8461 tccgccgtca tcatcacgct ggttcttttc gggcggatgc tcgaagcgcg cgcaaaagcc
     8521 agaacgacgc aggccatgag cgcgctgctg gcgctgcgac cggtagctgc tcatgtggag
     8581 cgggacgggc agatacggga tgttgacccg gcggatgtgg tggcgggcga tgttcttgtg
     8641 gtgcgaccgg gcgaacgtct gcctgttgac ggagtggttc tggacgggtt ctcctcggtg
     8701 gatgagagca tgctgacggg ggaaagcttc ccggtcgaga aaaaacagga tgatacggtg
     8761 accggcggga cattgaatgt cgcgggaagt ttccgatacc gagccacgcg ggtggggaac
     8821 gagaccacgc tggcccagat catccggctg gtggagcacg cgcagggttc caaggcaccc
     8881 gtacaacggc tggttgaccg gatatccgcg gttttcgttc cgacggtgat cgcgatttcg
     8941 gcggtgacgt ctgttgtgtg gtgggctttc gggccggagc cggcgctcca ttttgcggtg
     9001 ctgaacgccg tcgcggtgct gatcgtcacg tgcccctgtg cgcttggcct tgccacaccg
     9061 acggcaatca tggtgggaac ggggcgaggg gcagagatgg gcatcttcat cagaagtggc
     9121 gaaacgctgg aaagggttgg caggctgacg acggttgtgt tcgacaagac gggaacgctg
     9181 acacagggag aacctgttgt gacgggggta cacacagtgg gtaccctgcc agcggacgac
     9241 gtgctggttc ttgcggcgtc ggcggaggcc cggtcggagc accctctggg ggccgccgtt
     9301 gtcaggaaag cagcagagcg ttcgcttccg cttctttcgt gcgaggagtt ttcggctgag
     9361 tcgggccacg ggctgcgggc aatggttgcc aaccgggtgg ttctgatcgg caattccgcc
     9421 ctgatgcagg cgcagggcgt gcgcctggat gcgggaagga gcgttgcgga ggagattcag
     9481 gccaagggag ccaccgcggt gttcgtggcg ctcgatggcg cgctggaggc ggttttcgag
     9541 attgccgata cgccgaaacc gcacgccgcc gaggttatcg ccgcgctgaa acgcgacggc
     9601 ctgaaggtag tcatcctgac aggggattcg cgacggacgg ctgaagcaat tgcatcacaa
     9661 ctcggcgcgg atgacgtgat cgcggaggtg aaaccgggtg cgaaagcgga ttatgtcgcc
     9721 gccctgcaaa agagcgggga ggtcgttgcg atggtgggtg acggcgtaaa cgacgcgccc
     9781 gcgctcgccc tggcggacat cgggatcgca cttggcacag gaagcgatgc cgcgatggag
     9841 accgcaggga tcaccctcat ggggggcgac atacgcaacg tgcggaaagc gatcgcgctt
     9901 tcccgtgcga ctctgcggac gatacggcag aacctcttct gggcattcat ctacaacgtg
     9961 gcgggcattc ccatcgcggc gggcgttctt tacccggtga acggcatgct gctcgacccg
    10021 atgctggcaa gcgccgcgat ggcgatgagc agcgtgagcg tggtgacgaa cagcctcagg
    10081 ttgaggcggt ggaacgggtg agctttctga acgcaccccg tgtgcggcgg aaccgggctc
    10141 acggtgcgag tgcgagggaa atcagccccc gcagaagcgc atcatgaact gctgcgttat
    10201 cgcacgcaga cgttcgtcca gggtctcgta cacccgcgcg gaaatgtgtt cgggtacccc
    10261 accgtatccg gcctgcgcga ttccacccgt aatacacgca acggtgtctg agtctccccc
    10321 aagagaaacg gcagtgcgga tcgcgtcctc gaaatctgtg gagtcgaacc acgcacggat
    10381 cgcttgaggg accgttccct gcgaggactc atcaaacgtg tacgtcggcc ggatctcatc
    10441 aacgtgagcg ctcaggtcgt acccgaactc cgactcgaca aaggaccgta tctcatcctt
    10501 ggttttgccc ttacgcgcca gcagtatgga agccgcggtt gcctgcgccc cttttatccc
    10561 ttcgggatgg ttgtgggtta cttcggtaaa ctccttcgcc ttccgaagca cctgttcaag
    10621 gtcgtcgtac gcgtacccca cggggcttat gcgcatggca gcaccattcc cccagctttg
    10681 gtacggggac atatccgtgc tccgagccca tgtgtggaac gacgagccgt atccccggtc
    10741 gggataaagc cggtagaacg ttttcatgtt acgggcaaag ggcaccccgg agagaatgga
    10801 gtccgcgagg gcaacggtaa gaacagtatc gtcggtgaac gtgcatcgcg gtgaaaacag
    10861 aggaaaatcc ttggtcttga tgttatccca ttcgtacacc gaaccgatga tgtcccctgc
    10921 aagtgctccg atcatccgtc cctccgaaca ggcgccgtgc gatgcgctat ggaaacacgg
    10981 aggagcactc gcggggcgta cggcgttttg aggccgtcgg gcaggccggc gcggggcgcc
    11041 acaaaggtgt ggccgctgat ccacgcggtg ctgcttacgt cgaggcctat ctcaaggccc
    11101 gtgtaggtgc ttgagacacc cggatccagc gcattttcgg gcggacctcc ccaccccatg
    11161 aagtaccctg caaccgcccc cacgggtacg ccgtgcgagg agaccgaagt ccacgcgacg
    11221 tggtattcgc ggcttcccgt gtgctccatt cgcatgtacc ccccgtacca gccgacgaac
    11281 ggggacagga ggtgtttcga ggtctgccgg aaaccgagcc atcgccggca gggctcatat
    11341 cgcgccccca gagtaatcgc ggttacggag aagctgtttt tgaacgccgt cgtttcgggg
    11401 ggtgaggtgt acgttctgat gagcctcaat cccgccagtt tttccgttcg ttcgaaccgc
    11461 gtggataacc gctcgacgtt gaaatgcgtc gaccaccacc cgaagctccg cgacggtgtc
    11521 atctggtcca caccgaagag gaaccccgcg acgcgccccc actcgcccac cgtcccgccg
    11581 gtggcggcac ctgcgcggac ggtgtactgc gcgtgcgcac gggtggccga catgaggatg
    11641 gacgcgaagg ccgccgtgag gctcatcagc gcacgaagct ttgaggagaa acctgtcaca
    11701 ggttgaaacc gatcaaggta agtgaaccga ttgccagacc gagccctgcc tcggcggcga
    11761 tcactttcca cactgccagc cggtgcgcat cggagaggca caggatttcg atcagcagag
    11821 accagacggt gagcccgaac tgaacgccgt aatcaacggt gagtacgagg gcccgggggt
    11881 cgtccgatcc ggagatgagc gacggagtca gggctgagta gccgtaaccg gtcaccatga
    11941 tcgcccagag tgccagactc ggaatgcccg gggcaagagc ccacgcgacc gccgtccgtg
    12001 tcgccgtcca cgaccccaca ccgccgatcc gccgaagcac ccagtgcaaa atccgggcaa
    12061 tgacaaagac catgagcgca ccggccaagg cgcccgacac gagaatcagg agcgtcagtg
    12121 cctccggttc gagttcaacc agggacggaa ggagtgcgat ccgctccacg atccgacttg
    12181 taccaccggc cagtatgaga agccacagcc atcgccacgg tgcgtacaca gtcatggcgg
    12241 caaccgtctg tcgcgggtgc gtccagatgg ttcgccacgg gtgcagctcc gcggtcacac
    12301 cgtcctgtgg tgaagaaaac gccatcgtac ctcacgtgcc ttcgtcggac cccgcgtacc
    12361 atccattccg gccccctgaa gaaggtaccc accgaggcgg ggtttcgccg aggggcaacg
    12421 cctcgccgga acaatctgcg aagcaaagga gtgttgacga aatggcggtg tatgccacta
    12481 tggcattacc cattgcgcca ttacggcata aatcaaagaa aacaaccttc cgcgaaccgc
    12541 acggcacagg tattgcgttt ctccatgccc cgaaggtttc ggtcagtcat caagcgattg
    12601 ttcgtcggct gagatctgcg ggtcagacag taacgtccat cttgtcgctc gggagacgcg
    12661 tgtgaaggtc acaattcgtg aagagaagtc gaaggata
//
LOCUS       DAOSOO010000079        12685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_4949_length_12685_cov_6.168963, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000079 DAOSOO010000000
VERSION     DAOSOO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_12685_cov_6.168963"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="PLT86_07330"
     CDS             complement(<1..1367)
                     /locus_tag="PLT86_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT36459.1"
                     /translation="MGALRFGDLLAVVLYAVAMLWLGWWSQKRIKGTEGYFVGNRSLP
                     GWAVGLSILATAISSITFLAYPGNSYQGNWSRLVPGLMLPVATLIGVYFFVVFYRRTL
                     FVSAYQYFEQRFGNWGRSYASVLFTLGSLYRMGMVLYLLSIPIKVMFGLDISTTILFA
                     GIIVTIYTVMGGLEAVIWTDVAQGIILIAGGVITVLIAFIDVPGGAHEIISRGMAQGK
                     FDMAVSFDWDFTKETIWIFMLMGLVGNIQELSTDQTKVQRYQAAKSDKGAIGATLTVL
                     ACIPVWMLFMFVGTCLWAYYTDFPQLLPAGLKADYVYPHFILTQMPTFVAGMVISAVL
                     AAAMSTIDSSMNGSASVMLEDFYKRHFRPGKEDLHYLKVARYITIALGIIMMFIAWGL
                     SLLDANTILDIGFFIGAVMAGGLGGFFLMGFLFKHVNNKGAMVGVAAGVISILWCTLS
                     HLNPEL"
     gene            1602..4940
                     /locus_tag="PLT86_07335"
     CDS             1602..4940
                     /locus_tag="PLT86_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36460.1"
                     /translation="MNTPTDTLRTILSRVRAERRRAAALTLALATGAVGLAGLLVAVL
                     LETVGAFPPVGRFVLLGGWVGTTVFTAWFIFSRVRRAVGTHETIARDIENAYPEFNER
                     LIAGWEFATRPVSGSRSLADSVVREAALVLEKVDLRPLISWRPVARSAGAFFVTFAVV
                     CVSWFAFPDQIPSAFNRLVHPTVQFVTPPPFSFTLTPGNTRLVRGDTLTVKVRFSGSY
                     PRSAVVFSRDLQTQAWRSDIVSVGDSALLIWQALQLRSDIEYRVVAGELESPTYRVTV
                     VNPPAVERVRVVLTYPGYTGMSPDTLPENEGNIAALTGTVASFAVHPSKPVQRGELRF
                     FGRDTAVVALRKEGNVLAANWTVRHSGDYTIHLVDEFGYSDRNPIAFRITTVADEYPS
                     VRITEPEQESDLGADMLVPLVVEARDDFGFDRAELVYRGPDGRPKTLRLLLTTLERGR
                     AEVHYTWDVGELDLLPEERVYYRVAVYDNDRISGPKRVESEEQVLRFPSAAEVFAEAQ
                     QQQQQAIVDLSAITRQADEVARQLEAARRELLKNPSLSWETRQASQRALQQQRELNRE
                     LQAVADSLRQSLERLQKHDILAPETMEKLVRIQEMMEDIVTPELRQTLSELQNSLQQM
                     ADPRQVQQALQRFAQSREQFEERLDRTLRLLQEAQAEQQLDAMSKRLRELARMQRDVA
                     ESFDRQARETLANREQILGGQVSETQDRLERMAEEMRGLPASPSDSLRSVAEQLDRNR
                     IAERLQQVGNRLAQGGEPARAQNKGEAARLAEDLERTAGAMQRTAEARRNARKREIAA
                     QLDRAATDLLRLSMMQEDLRNRTRQRRGSAGASREGEEQVDLLQGTSGVIRRVMEASQ
                     ETFLIPPETLQELGTALIEMKGASEALEQNNTASAVPRQQNAMASLNRAVDMIRSAQR
                     NAQTGESGTGLEELLRQLGEAARRQKQLNDAMQALGQQPSLSPSDLQMLGQMAAEQRA
                     LSQLMQQLAEALKRHREVLGRLGDLAGEMESAAEEMERRQLGPRLADRQQRILQRLLD
                     AQRSLQQDKVSEQRVARTAGHQPLRPPGALPEDLGERKAALREALLDALKADFPPEYR
                     TWIRSYYEWLMGREARESAPQSAP"
     gene            5115..5585
                     /locus_tag="PLT86_07340"
     CDS             5115..5585
                     /locus_tag="PLT86_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT36461.1"
                     /translation="MKLCDLLTDSAVAVALPAKDKSDLLRKLVDLATKSGSVQDPAGA
                     LHAVEEREKIMTTGIGHGVAIPHGKTNAVRDLVAAFAVTEEGVDFGSLDGEPVRLAFI
                     LLGPKEPSGPHIRMLSRISRLMNREDLRAKLINAKSVAEVRALFTDAEHDMFDL"
     gene            5723..8806
                     /locus_tag="PLT86_07345"
     CDS             5723..8806
                     /locus_tag="PLT86_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT36462.1"
                     /translation="MEIQAAKERFVSPPATNRPVIFWFWNDHLHPERLVWQYDRLIEA
                     GMAGAVMHARGGLESAEYLDERWFAAVDAVVKHAAEKGTVVWIYDELGWPSGSAGGRA
                     LVGLPREMQLGHLKLFDITPEPGKDYSRLPGSPVAAFRVVRSDPDHGFQRRYDKSVNL
                     MRDEITCEELPKDVAPSAFVGTRLLLFRHVIDGGLIDYYNAAATEVFLKSTHEEYFRR
                     FGAHFGTTITHSFMDEAGSMAGIGMLPWSPVFESEFEKRRGYAIRPHLAALLFEIPGF
                     QSVRYDYWSLTAELFREGFGVPMHQWCERHGIKYSGHYVFETSLKEATRQLGSAMPLY
                     EFQGLPGVDVLGNDFYTHRFEIEAHAYYTAMVKQASSVSNQLRDGQLLSESYGVGGHA
                     MGLESMQVATFWQMALGVTVISQHAAFYSMRAERKEDCPPVIGWQEPYWKFARPHIDS
                     ISRTSWLLSQGRRRCNVLYLHPQAGMQASYRQCRVKDEYKYESYILNADMPYEMIDKH
                     FSLLGVALLDAQIDFEYGDEEILAAHGVAEYGAFKVGKQTYETVLVQPMLNIRSSTLA
                     LLEAFAAQGGQIVLVGSAPGFVDGKSSRRALDFFSAHARRVTEGVDFFDYAPAVEVLS
                     ALGCRTVETSSPVPQIKVHRRLWDGRDIVFLANISRRTVETEVTFVPQVTGTVEEWNV
                     ADGTTHPVAHCTAGQPLTLAVKWHPKQARTFVTMEAAVQLPPVVTWSEERRLTPQWAG
                     TRTGPNALLLDACVVQDLSGASRLLSVGDARKTVLAAQMKNGSGDKPFAARFPVRVSE
                     TDPPRSACELAIELGTGPRLAFNGVPVPVNTLGWVIDPAIERIALPGLNPGENTLFVE
                     AAYGKATEFETPWLLGDFAVRTGDNVSFTVEKAAQTVPIGPWHELGLPFYAGTVVYRA
                     EVELELSDAQRVVVDLAGLAGSAGVSVNGQFVTTVLWPPYECDITSAVKPGTNVIEIE
                     VANTLRNLLGAHYVENEEIRTGAPTNLYTAPFGTPKKFLPYGLLTAPEIVISRCS"
     gene            8826..11036
                     /locus_tag="PLT86_07350"
     CDS             8826..11036
                     /locus_tag="PLT86_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT36463.1"
                     /translation="MVRNWMPLAHGWKFLRDECSHAWKADFDDSLWETVSVPHSFNAA
                     DTFVPEPGYPDYYRGGTWYRRILPRTPAGARVELTALGVFSVTDVWVNGKHVGKFMGG
                     YTGFCTNLTPYLKRAGENVLALRVTNQHNPDVLPGLDFPDYNLYGGIYREIGLTITAP
                     LHIPDRGIVLTTPRVSRESAAIHLNVRVANDQKRSASGTVVVDVKSPGGRVVARGRQH
                     YVVAAHTERLVTVPLPQISQPKLWSPDNPTLYSVHVKLLEGDEVADAQSLRFGFRWFC
                     FDADKGFFLNGRHLKLHGVNRHQDFAGLGNALPANFQAHDVKLIKEMGANFVRCSHYP
                     MHPAFLDACDEMGVIVLEEIASWQWIGGSRFTENAIAMMREMIARDRHHPSIILWGLL
                     NEGRSSDLFRTLNATAKQADPWRATIYADNEPEEGLELGTVFVPDVLGLNYKVPHLDD
                     LRAILKGIKLSNTEHTNANTGERRKPDGTLFSDEESQIWQIERILHDIAEFEKREWIA
                     GNALWCMHDYGTEYAVSRPIQKSGVFDAWRIPKAAAYAIRAWWSKEPFVRIAEHWTYP
                     GTEGSPRKVIVLSNCESVELLVNGRSFGSRTRDPHAYWFEWEVPYSPGEIVAVGHTPS
                     GDLRDLRRTAGAPSAMVIQTLDESLPANGTDITLVTVSVVDVHGTPVPAAEAEVRFSV
                     AGGGTFFGLYGNASVSTRNGVGRIAYRSARKKGTVRITASADGLAPASVELKLI"
     gene            11132..12427
                     /locus_tag="PLT86_07355"
     CDS             11132..12427
                     /locus_tag="PLT86_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36464.1"
                     /translation="MLDPRLVERTENLRLVLGCVRKDARNPLLIEDQPWEVRFDNLYP
                     NVHWSREKGLFECWYNPFIVCEATSNTPDEEKRRIPYRPGKREMGVCYAQSRDGIIWE
                     KPALGLIEFEGSTANNLVMRDIHGVGVYYDRFESDPARRYKSLTSVGTGTSPDGLRWT
                     LRERPDIGAIGDTHNNLFWDKRTRRYVGFTRLWKDRQRIVARTESPDFVSWTRAEEVL
                     RASEDSPHRQIYELIVFPCSSGYLGIAAVLDTESDCVDCELAWSPDTIVWSRVAEGSP
                     LIPRGEEGSWDHGCIYAAAYPIITEKEVLLYYAGGNDTHMSWRKTGLGLARLRPDGFA
                     GWRPVGTPDRGELVTTSIACSAPNLTVSADAEGGSIRAEVVGVEGFSLESCRRVSADV
                     TGCVVTWGEGRTLAPFVGKTITLRFELLNATLYSIGFSG"
     gene            complement(12450..>12685)
                     /locus_tag="PLT86_07360"
     CDS             complement(12450..>12685)
                     /locus_tag="PLT86_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT36465.1"
                     /translation="GAAQHGFDLQLSGHTHGGQFYPWVFLVPLQQPYVAGLHRHENTW
                     VYVNRGAGYWGPPLRLGAPSEIALIRLIGRADA"
BASE COUNT         2655 a         3558 c         3987 g         2485 t
ORIGIN      
        1 agttccgggt tgaggtggct caacgtgcac cagagaatgg aaatgacacc ggctgcgacg
       61 cctaccatcg cgcctttgtt attcacgtgt ttgaacagga agcccataag gaagaacccg
      121 ccgagcccgc ccgccatcac cgcgccgatg aaaaacccga tgtcgaggat cgtgttcgcg
      181 tcgagcagcg acagacccca cgcgatgaac atcatgatga tgccgagggc gatggtgatg
      241 tatcgcgcga ccttgagata gtgaagatct tccttcccgg ggcggaagtg ccgcttgtaa
      301 aaatcctcga gcatcaccga cgcagagccg ttcatgctgg aatcaatggt ggacatcgcc
      361 gcagccagta ccgcggaaat gaccattccg gcaacaaagg tcggcatctg cgtgagaatg
      421 aaatggggat acacgtagtc ggctttcagt cctgccggaa gaagctgagg aaaatcagtg
      481 tagtacgccc agaggcaggt gcccacgaac atgaacagca tccagacggg aatacacgcg
      541 agcacggtga gagttgctcc gatggctcct ttgtccgatt tcgccgcctg ataccgctgc
      601 actttcgtct gatcggtgga aagctcctgg atgtttccca caaggcccat gagcatgaaa
      661 atccagatgg tctctttcgt gaagtcccag tcgaaagata cggccatgtc gaacttgccc
      721 tgcgccatcc ctcgggatat gatctcgtga gcgccacccg ggacgtcgat gaacgcgatc
      781 aataccgtaa tcactccgcc ggcaatcagg atgattccct gtgcgacgtc cgtccagatc
      841 accgcctcaa ggccgcccat taccgtgtag atcgtcacaa tgatgccggc gaacaggatg
      901 gtggtggaaa tgtcgaggcc aaacatgact ttgatgggga tcgaaagcag gtaaaggacc
      961 atccccatgc ggtacagcga accgagcgtg aacagcacgc tcgcgtaaga ccttccccag
     1021 ttcccgaacc gctgttcaaa atactgatac gcgctcacga agagcgtccg tcggtagaac
     1081 acgacgaaga aatacacgcc gatgagcgtg gcaacgggca acatgaggcc aggaacaagc
     1141 cgcgaccagt tgccctggta actgttgccg gggtacgcga gaaacgtgat ggaactgata
     1201 gcagtggcaa gtattgaaag gccgactgcc cagccgggca gagatctatt tccgacgaag
     1261 taaccttctg tgcccttgat gcgcttttgc gaccaccaac caagccacaa catggcaacc
     1321 gcgtacagca cgactgccag caaatctccg aatcgtaacg cccccatacg cgtacctccg
     1381 cccgcaaagg ttcgttgtgt gtaattatca agacgcggaa atgagagatg aatataggcc
     1441 tatttgctcc ggagggcata cccttcctac ggaggggtac ccttcgggta tcaccacggc
     1501 gacgcgtccc aaaggcattg gctctgcgtt cgttggaacc acctccactg ccgggctatt
     1561 gtgtacacag gacgatttgt gcacgacgca acggaaccat gatgaacact cccactgata
     1621 cccttcgtac gattctgtcc cgtgttcgcg cggaacggag gcgtgcggct gcgttgacac
     1681 tggcactggc cacgggcgct gtcggcctgg cagggctgct ggttgcggtg cttctggaga
     1741 cggtgggcgc atttccgccc gtgggtcgtt tcgtgttgct tggcggatgg gtgggtacga
     1801 cagtttttac ggcctggttc atcttttccc gtgtacggcg ggcagtggga acacacgaga
     1861 cgatcgcccg ggatatcgaa aacgcatatc cggagttcaa tgaacgattg atcgcgggct
     1921 gggagttcgc gacgcgtccg gtttccgggt cgcggagcct cgccgactcg gtagtacgtg
     1981 aagccgccct cgtgcttgag aaggtcgatc tgcgcccgct gatttcgtgg cggccggtgg
     2041 cgcgttccgc gggtgcgttc ttcgtcacct ttgctgtggt ttgcgtttcg tggttcgcct
     2101 tcccggatca gataccctct gcgttcaacc gtttggtcca tccgacggtg cagtttgtta
     2161 cgccgccccc gttctcattc acgctgaccc ccggcaacac gcggctggtt cgcggcgata
     2221 cgctgaccgt gaaggtgcgt ttcagcggat cgtacccccg cagtgccgta gtgttttcgc
     2281 gtgaccttca gacgcaggcg tggcgaagcg atatcgtcag tgtgggagac tccgctctgc
     2341 tcatttggca ggcgctgcag ttgcggtcgg acattgaata ccgtgttgtg gcgggagaac
     2401 tggaatctcc gacatatcga gtgaccgtgg tgaacccacc cgcggtggag cgcgtccgcg
     2461 ttgttctcac ctacccgggt tacacgggaa tgagccctga tacgctgccg gaaaacgagg
     2521 gtaacattgc cgccctgaca ggtaccgtcg cgtcgtttgc cgtgcacccg agcaaaccgg
     2581 tccagcgggg agaattgcgt ttcttcgggc gagacacggc ggtggtggcg cttcgaaaag
     2641 agggcaacgt tctcgccgcc aactggacgg tacgacactc gggtgactac acgattcacc
     2701 tcgtggatga gttcgggtac tcagaccgca atccgattgc attccgtatc accacggtgg
     2761 ctgacgagta tccctctgtg cgcattacgg aaccagaaca ggaatcagat ctgggcgcgg
     2821 acatgctggt tcccctcgtg gtagaagcgc gcgacgattt cgggttcgat cgtgccgaac
     2881 tcgtgtaccg cggccccgac ggcagaccga agaccctgcg cctgctcctt accacacttg
     2941 agcggggacg cgcggaggtg cattacacat gggacgtcgg cgaactggac ctgctgcctg
     3001 aggaacgggt gtattatcgt gtggcggtct atgataacga ccgcatatca gggccgaaac
     3061 gagtggaaag cgaggagcag gttttgcgat ttcccagcgc cgctgaggta ttcgcggagg
     3121 ctcagcagca acagcaacag gcgattgtgg atctgagtgc gattacgcgg caggcggacg
     3181 aggtagcacg gcaacttgag gcggcgcgcc gggagcttct gaaaaatccg agcctttcat
     3241 gggagactcg ccaggcatcg cagcgtgccc tgcaacaaca acgcgaactg aacagggaat
     3301 tgcaggcggt tgcagactcg ttgcggcaat ctctggagcg gctccagaaa cacgatattc
     3361 tcgctccgga gactatggag aagctcgtgc gcatccagga aatgatggag gacatcgtta
     3421 cgccggagct tcgtcagacg ttgtccgagc tccagaattc gctccaacag atggcggatc
     3481 cgcgtcaggt gcaacaggcg ctgcaacgat tcgcgcagag ccgcgagcag tttgaggagc
     3541 ggcttgaccg gaccctcagg ctcctgcaag aggcccaggc ggaacagcag ctcgacgcaa
     3601 tgagcaagcg actgcgggag cttgcacgaa tgcagcggga cgttgcggaa tcgtttgacc
     3661 ggcaggcgcg ggagacgctt gccaaccggg agcagatact cggcgggcag gtttcagaaa
     3721 cgcaggaccg gctggaacgc atggcggagg agatgcgcgg cctgcctgcg tctccttctg
     3781 atagtcttcg atcggttgcg gaacaactgg accggaacag gattgcagaa cgcctccagc
     3841 aggtcggaaa caggctggcg caggggggag aaccggcgcg tgcgcagaac aaaggtgaag
     3901 cggcacggct ggcggaagat ctcgaacgga cagccggagc catgcagcgc accgctgaag
     3961 cgcggcggaa cgcgcgcaaa cgggaaatcg cggcgcaact tgaccgggcc gcgacggacc
     4021 ttctgaggct ttccatgatg caggaggatc ttcggaaccg cacgcgccag cgcagaggca
     4081 gtgccggtgc ttcccgtgag ggcgaggagc aggttgacct tctgcaggga acatcggggg
     4141 tgatacgccg tgtgatggaa gcgtctcagg agacattcct gatccctccg gaaacactgc
     4201 aggaactggg caccgcgctg atcgagatga aaggtgcgag cgaagcactt gaacagaaca
     4261 atacggcttc tgcagtccca cgccagcaga atgcgatggc atcgctcaac agggcggtag
     4321 acatgatccg ttcggcacaa cggaacgcgc agactggcga atcggggaca ggtctggaag
     4381 agcttctgcg ccaacttgga gaggcggccc gtcgccagaa gcagttgaat gacgcgatgc
     4441 aggcgctggg acaacagccg tcgctgtcac cgtcagacct ccagatgctt ggccagatgg
     4501 cagccgagca acgggcgctc agccagctca tgcagcagct tgcggaggcc ctgaaacgcc
     4561 acagggaggt tctcggacgg cttggggatc ttgcgggtga gatggaaagc gcggcggagg
     4621 agatggagcg gcggcaactc gggcctcgtc tcgcggatcg gcaacagcgc atcctccagc
     4681 gtctgcttga cgcgcagcgt tcgctgcagc aggataaggt gagcgagcaa cgggttgcca
     4741 ggacggcggg ccaccaaccg ctccgtccgc caggcgcctt accggaagac ctgggcgaga
     4801 gaaaagccgc tctgcgggaa gcgctcctcg acgcgctgaa agcggacttc ccgccggagt
     4861 accggacgtg gatccggtcg tactatgaat ggctcatggg gcgcgaggcg cgcgaaagtg
     4921 cgccacaatc ggcaccctga tgagcggcgg cgaggggggt atggcatata acactggccg
     4981 agtgttattt tgtacattcc cattcacccc cttggctttc cggctgcacg ccgtatcggg
     5041 agtgacgaag cgacaaaccc ggaggcaggt gcgcaacctt tggacaagtg ccgtggaaag
     5101 gcaggaatta tcggatgaaa ctttgcgact tactgactga ctcggctgtc gctgttgcgc
     5161 tgccagcgaa ggacaagagc gatctcttgc ggaaacttgt tgatctggca acgaaaagcg
     5221 gttccgttca ggacccggct ggtgcgctgc atgccgtgga ggaacgggaa aaaatcatga
     5281 cgaccgggat aggtcacggt gtcgcaattc cccacggaaa gaccaacgcc gtgcgcgatc
     5341 ttgtcgctgc gttcgcggtg acggaagagg gagtggattt tggttcgctg gatggcgaac
     5401 cggtgaggct cgcgttcatt cttctggggc cgaaggagcc gagcggcccc cacattcgga
     5461 tgctgagccg gatatcacgc ctcatgaacc gggaggacct gcgcgcaaag ctcatcaatg
     5521 cgaaatcggt cgcggaagtg cgtgccctgt tcaccgatgc ggaacacgac atgttcgatc
     5581 tttgaaggtt gaacagaaca aaggcaatcg gggtcgtgcg gtgcaggcgt agcagcacgg
     5641 ccccttgtgt ttttcccgcc ctcctcccgt tgtttctttc gagacaatcc tcacgtgcaa
     5701 aacccgttga aagggttctt ccatggaaat tcaggcggca aaagaacgct ttgtctcccc
     5761 tccggccacg aaccgcccgg tcatcttctg gttctggaac gatcacctgc accccgagag
     5821 gctcgtctgg cagtacgacc gcctcatcga agccggcatg gcgggagcag taatgcatgc
     5881 cagaggggga ctcgaaagtg cggaatacct cgatgagcgc tggttcgccg cggtggatgc
     5941 ggttgtcaag catgccgccg aaaagggtac cgtcgtctgg atctatgacg aactgggctg
     6001 gccatccgga agcgcaggcg ggcgcgcact tgttggcctt ccccgggaga tgcagcttgg
     6061 ccacctcaag ctgttcgaca ttacaccgga gcccggaaaa gactactcgc gccttcctgg
     6121 aagcccggta gccgcgttcc gagttgtacg gtcagatcct gaccacggat tccagcggag
     6181 gtatgacaag agcgtcaacc tcatgcggga cgaaattacg tgtgaggaac tcccaaagga
     6241 cgttgcccca tctgcattcg tggggacgcg gcttctcctg ttccgtcacg tgatcgacgg
     6301 cggcctgatt gactactata acgcggccgc aacggaagtc ttcctcaaat cgacccacga
     6361 ggagtacttc cggagattcg gcgcccactt cgggacaacc atcacccaca gcttcatgga
     6421 cgaggccggg agcatggcgg gtatcgggat gttgccatgg agcccggtgt ttgagtcgga
     6481 atttgagaaa cgccgtgggt atgcgattcg tccccatctc gccgcgttgt tgtttgaaat
     6541 ccccggcttc cagtcggtcc gttatgacta ttggtcgctg acggctgagc ttttccgcga
     6601 gggctttggg gttccgatgc accagtggtg tgaacggcac gggatcaagt acagcggcca
     6661 ctacgtgttc gagacgtcgt tgaaagaagc gacgcggcag cttggcagcg cgatgccgtt
     6721 gtatgagttc cagggccttc cgggggtgga cgttcttggg aatgatttct acacgcatcg
     6781 gtttgagatt gaagcgcatg cgtattacac cgcaatggtg aaacaggctt cgtcggtttc
     6841 caaccagctt cgggatggcc agctcctgag cgagagctac ggcgtgggcg gccacgcgat
     6901 ggggctcgaa agcatgcagg tggccacctt ctggcagatg gcgctcgggg taacagtcat
     6961 cagccagcat gccgcgttct acagcatgcg cgccgaacgg aaggaggatt gcccgccggt
     7021 tatcggctgg caggaacctt attggaaatt cgcccgcccg cacattgatt caatcagtcg
     7081 cacgagctgg cttctttcgc aggggcgccg tcgctgtaac gttctttacc tgcacccgca
     7141 ggcgggaatg caggcgagtt accggcaatg ccgcgtcaag gacgagtaca agtacgagag
     7201 ctacatcctg aacgcggaca tgccgtacga gatgatcgac aagcattttt ccttgctggg
     7261 cgttgctctg ctggacgcgc aaattgattt cgaatacggc gacgaagaga ttctggccgc
     7321 acacggcgtt gcggagtatg gggcgttcaa ggtaggaaag cagacgtacg agacggttct
     7381 ggtgcaaccg atgctgaata tccgttcgtc gacgcttgct cttctggaag cctttgccgc
     7441 gcagggaggg cagatcgttc tggtagggtc ggctcccggc tttgtggatg ggaagtcctc
     7501 gcggagggct ctggatttct tctccgctca tgcccggcga gttaccgagg gcgttgattt
     7561 cttcgactac gcgcccgcgg tggaagtgct ttctgcgctc gggtgccgca ccgtggaaac
     7621 cagttctccg gttccccaga tcaaagtcca tcgccgcctc tgggatggca gggatatcgt
     7681 gttcctcgcg aacatctccc gccgcacggt ggaaacggaa gtcacgttcg ttccgcaggt
     7741 gacgggcact gttgaggaat ggaatgttgc cgacggcacc acacaccccg tggcgcactg
     7801 tacggcgggc caaccgctca ctctggcagt gaaatggcat ccaaaacagg cgcgtacctt
     7861 cgtaacgatg gaagctgcgg tgcaactccc gcccgtggtt acgtggagcg aagaacggcg
     7921 cctgactccg cagtgggcgg gaactcgaac cggcccgaat gctctgcttc tggatgcctg
     7981 tgtcgttcag gatttgtccg gcgcttcgcg cctgttgtcc gtgggtgacg caagaaagac
     8041 cgtccttgcc gcgcagatga agaatggctc gggcgacaag cccttcgcag ccaggtttcc
     8101 ggtgcgcgtt tcggaaaccg acccaccgcg ctcggcgtgc gaacttgcca tcgagcttgg
     8161 aaccggaccg cgtctggcat ttaacggcgt accggttccc gtgaacaccc tcggctgggt
     8221 gatagacccg gctatcgaac gaatcgccct gcccgggctc aaccccggtg agaacacgct
     8281 tttcgtcgaa gctgcgtacg gaaaggcaac ggaattcgag accccgtggc ttctcgggga
     8341 tttcgccgtc cggaccgggg acaacgtgag tttcaccgtg gaaaaggcgg cacagacggt
     8401 tccaatcggt ccatggcacg agctcggtct gccattctac gcggggacag ttgtctatcg
     8461 cgctgaggta gagctcgagc tttcggatgc gcagcgggtc gtggtggatc tggcgggcct
     8521 cgccggttcg gcgggggtca gtgtgaacgg ccagttcgtc accaccgtgc tgtggccccc
     8581 gtatgagtgc gacattacct cggcggtgaa gccgggcaca aatgttattg agatagaggt
     8641 ggcaaacacg ttgcgtaacc tcctcggtgc gcactatgtc gagaatgagg aaatcagaac
     8701 gggggcgccg acaaatctgt acaccgcgcc tttcggaacg ccgaagaagt tccttcccta
     8761 cgggcttctc accgcgccgg aaatcgtgat ttcgagatgc agttgacagc atgggggtga
     8821 tacacatggt tcggaactgg atgcctctgg ctcacgggtg gaaattcctg cgggatgagt
     8881 gctcacacgc ctggaaagcc gattttgatg attctctgtg ggaaacggtt tcggtccctc
     8941 actcgttcaa cgccgccgat acgttcgtgc cggaacccgg gtacccggat tactaccgcg
     9001 gaggaacctg gtatcgcagg attctgccgc gaacacccgc cggtgcgcgc gtcgagctca
     9061 ccgcgctggg agtgttttcg gtaacagatg tgtgggtcaa cgggaaacac gtcggaaagt
     9121 tcatgggagg gtataccggt ttctgcacga acctgacacc gtacctcaaa cgagccggcg
     9181 aaaacgtgct cgccctccgg gtgacgaatc agcacaaccc cgacgttctg ccgggtctgg
     9241 atttccccga ttacaacctt tacggtggca tttaccgtga gattggtctc accatcacgg
     9301 ctcctctcca cattcccgat cgcggcattg ttctcacgac gccacgggtc tcgcgagagt
     9361 cagccgcgat ccaccttaac gtccgcgtcg cgaacgatca aaaacgctcc gcttcgggca
     9421 ctgtggtagt ggatgtaaaa tctcccggcg gacgcgtcgt cgcacgtggg agacagcact
     9481 atgtggtggc cgcacacacg gagcgtctgg ttaccgtccc gctgccgcag atatcgcaac
     9541 cgaagctctg gtcaccggat aacccgacgc tgtattcagt ccacgtgaaa ctcctggagg
     9601 gggacgaagt agcagatgct cagtccctcc gctttggatt cagatggttc tgtttcgacg
     9661 cggataaagg gttcttcctg aatggcaggc acctcaaact gcacggtgtg aaccggcatc
     9721 aggacttcgc cggtctggga aacgcacttc cggcgaattt ccaggcgcac gatgtgaagc
     9781 tgatcaaaga aatgggtgcg aacttcgtgc gatgcagcca ctatcccatg catcctgcgt
     9841 ttctggatgc ctgcgatgag atgggggtta ttgtgctgga ggagattgcc tcgtggcaat
     9901 ggatcggtgg aagcaggttc accgaaaacg ccatcgccat gatgcgcgag atgatcgcgc
     9961 gcgacaggca ccacccgtca atcatcctct ggggcctcct caacgaaggc cgcagcagcg
    10021 atctgttccg tactctgaac gcgaccgcga agcaggcgga cccgtggcgg gctaccatct
    10081 atgccgacaa cgaaccggaa gagggtcttg agctcggaac ggtattcgtg ccggatgtcc
    10141 ttggtctgaa ttacaaagtg ccgcatctgg atgacctgcg cgcgattctc aagggcatca
    10201 agctctccaa tacggaacac acgaacgcga ataccggtga gcgcagaaag cccgatggga
    10261 cgctcttttc cgatgaagag agccagatct ggcaaatcga gcgcatactg catgacattg
    10321 cggagttcga gaagcgcgaa tggatagcgg gcaacgcgct ctggtgtatg cacgattacg
    10381 gtacggaata cgcagtttcg cggcccatac agaaatcggg ggtgttcgac gcgtggagaa
    10441 ttccaaaagc ggcggcatac gcgatcagag cctggtggtc gaaagaaccg tttgtgcgca
    10501 ttgccgagca ctggacgtac cccggcaccg agggcagtcc ccgcaaggtg atcgtgctga
    10561 gcaactgtga atcggtcgaa ctgctcgtga acgggcggtc cttcgggagc agaacacggg
    10621 acccgcacgc gtactggttc gaatgggagg tgccctattc ccccggcgaa atcgttgcgg
    10681 taggtcacac tccttcaggg gacctccggg atttgcgccg tacggcggga gctccctccg
    10741 caatggttat acagacgctc gacgagagcc tccccgcgaa cgggacagac atcacactgg
    10801 ttactgtgtc ggtcgttgac gtacacggga ctccggttcc ggcagcggaa gccgaggtac
    10861 ggttctcggt ggcagggggc gggacatttt tcggcctgta cggcaacgcg agcgtttcaa
    10921 cgcgaaacgg agtaggacgc attgcgtacc gctcagcccg gaaaaagggc actgtgcgca
    10981 tcactgcttc ggcggacggg ctggcgccgg catctgtcga gctgaagctg atctgacgcg
    11041 cagaaatgcg ttgtggtgaa cagctcaaag ggaattgatc aggcccgttt gcgatggtct
    11101 ttacggaggc gctttgcaac gaacattttg cttgcttgac ccacgtctcg tggaacgaac
    11161 ggagaacctt cgcctggtct tgggttgcgt tcggaaggac gcgagaaacc ctcttctgat
    11221 cgaagatcag ccgtgggaag tgcggtttga caacctctat ccaaacgtgc actggtcgcg
    11281 cgaaaagggg ctgttcgaat gctggtacaa ccccttcatc gtgtgcgagg cgaccagcaa
    11341 cacgccggat gaagagaagc gccgaattcc ctaccgtccc ggaaagcgag aaatgggggt
    11401 ctgctacgca cagagtcggg acggtattat ctgggaaaaa ccggctctcg gacttatcga
    11461 gttcgaagga tcaactgcga ataatctcgt catgcgcgac attcacggcg ttggtgtcta
    11521 ctatgacagg ttcgaatccg atcctgcgcg tcggtacaaa tcgctcacct cggtcgggac
    11581 cggaacgtct ccagatggtc ttcgatggac gctgcgagag cgccccgata tcggcgcaat
    11641 cggtgatact cacaataacc tgttctggga taaacgtacc cggcgttacg tcgggttcac
    11701 ccggttatgg aaagaccggc agcggatcgt agctcgaacg gaaagcccgg atttcgtttc
    11761 gtggacacgg gcggaagaag tacttcgggc atcggaggat tcaccgcacc ggcagatata
    11821 cgagttgatc gtctttccct gctcgagtgg ctacctcggg attgctgccg tgcttgacac
    11881 tgagagcgac tgcgtagatt gcgaacttgc gtggagccct gatacaatag tctggagccg
    11941 tgttgccgaa gggtctcccc tgattccgcg gggagaggaa ggctcgtggg accacggatg
    12001 catctatgcc gccgcatacc cgatcataac ggagaaggag gtgcttctct attatgccgg
    12061 tggcaatgat acccacatga gctggcgcaa gaccgggctg gggcttgccc gtctccgccc
    12121 ggatggtttc gccggatggc gccccgttgg gactccggac agaggggaac tcgttacgac
    12181 ttcgatcgcc tgttcggcac cgaatctgac cgtctccgct gatgccgaag gtggctcgat
    12241 tcgggcggaa gtagtgggtg tggaaggctt ttcgctcgag tcgtgtcgga gggtttcagc
    12301 ggatgtgaca ggctgcgttg tgacgtgggg cgaagggagg acgctcgccc cctttgtcgg
    12361 aaagacgatc acgctccgct tcgaacttct caacgcgacc ctttattcga tcggcttttc
    12421 gggatagaac gcgtccggat gaggcgaaat caggcgtccg cgcgtccgat caggcggatg
    12481 agtgcgattt cggaaggcgc gccgagccga agtggagggc cccagtatcc tgccccgcga
    12541 ttcacgtaca cccacgtatt ctcgtgccga tgaagcccgg ccacgtacgg ctgctgcaac
    12601 ggtacaagaa acacccaagg atagaactgt cccccgtgcg tatgtcccga aagctggaga
    12661 tcaaaaccgt gctgagcggc tccgt
//
LOCUS       DAOSOO010000080        12526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5021_length_12526_cov_5.535883, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000080 DAOSOO010000000
VERSION     DAOSOO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_12526_cov_5.535883"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLT86_07365"
     CDS             complement(<1..291)
                     /locus_tag="PLT86_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36466.1"
                     /translation="MPPVAEPAIVPPAPKRKQEGKWFLNALSVVLLGYAVFGKPFAYL
                     GFPPLYVGEMALALGVLAVLRYPSMLGAMVTAPLFPLLAIFVLWNAACTIPYI"
     gene            complement(342..3524)
                     /locus_tag="PLT86_07370"
     CDS             complement(342..3524)
                     /locus_tag="PLT86_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HOT36467.1"
                     /translation="MADSETRLFEAAVRANWSAYQKVIQGLAPAWDYCVITSANERQA
                     RAYESELDWRRLRGKLPPSTRFLVVPDPDGQRIGSGGATLNALRAIFEDKSARGEVAD
                     ESAWSKTRILIIHSGGDSKRVPHLAATGKAFGVLPLLDPEGQPITPFDQLFVAVAGCA
                     GDLSAGMMVLSGDVLLVFDHRAIRANELRGVRGVAFNALEDTAAHFGVFVAAQDGSVR
                     LFLQKPDHQALEVSGAFGSSGEVKADTGILLFDESATAACARLAGLHGGADLVRSARS
                     LGSRIELYEDFAFALARETDHAVYVAGGGACQIRETIWNSLHQFHFDHVTVSPIRFVH
                     IGTTEQYLDVPREAENHGYGYAVARSVYASGTTPPAKSCLIETVLRDNTSVIGEDAVL
                     IGCHLRRATVGRRSILSGLVSPDQDISLGDECVCAVIPLADDEAGDYAILLYGLKDNP
                     KLSADEAGCTFCNTAMSSWMDAAGINRTDLWDVGDQQTIWDARLFPVLSTLEDITEFL
                     WMPLRPPTKREQERWKTVRRLSMAEAFKKADCGRIISNRDAIRTAALCAQVRERLGTD
                     NDVRPLFGAADTRALCASAANEIRAFAETAPSPFLKARAFKAASDLFNERLRSISAHA
                     HVDSGAVSIPSGALGAWREFTAPYVHQWATDTEEAGSLATLARVHEELAFLQVAQVIE
                     SHSLRRGAQPHLAVSPGTRVVAEVPVRIDLGGGWTDTPPYSLEKGGVVANVALLLNGK
                     RPIRVTAYPILEPVVVLTSTDIGYSARITRCEPIVSYNDPGDPVALHKAALVLLGIIP
                     TGAEGPLEPYLQAFGAGIAVESACDLPKGSGLGTSSLLAIGLVAALNKICGITRQMDD
                     LFQDVLCLEQMLTTGGGWQDQIGGAVGGFKLIETLPGVVQKPSIRTIEADSQALHEFE
                     DRLVVYYTGAQRLAKNILRGVVGRWLSRDQEVVTALQDLHQVALDLWDAMRNGDIDRI
                     GALMNRAWRLNNVLNPGSSSPAINDLMDDISHLCSGAKLVGAGGGGFLIALAKSRAAA
                     AELRERLENRSPEGRVYNHTIAHDGLVVTT"
     gene            complement(3517..4359)
                     /locus_tag="PLT86_07375"
     CDS             complement(3517..4359)
                     /locus_tag="PLT86_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT36468.1"
                     /translation="MNAQSTTRLAQMPQKALESRFIVGTRVDATSYEDAVDRCISWAR
                     EGESRYVCVSTVHMVMEGHDSPAYQHVVNSADLVTPDGVPLVWALRWLGIKGVTRVYG
                     PELTPQLLKKAADHRIPVGFFGGGTQAALDRLVSIVRERFPHLVITYAYAPPFHPLSP
                     DEDETIVREITDSGARVLFVGLGCPKQERWMHEHRGRLPAVMVGVGAAFDFLAGVKPQ
                     APRLVQKMGMEWLFRLATEPRRLWKRYLKHNPRFVFFFLLQVLGVRQFAPIGAIGLSE
                     GTNG"
     gene            4555..6168
                     /locus_tag="PLT86_07380"
     CDS             4555..6168
                     /locus_tag="PLT86_07380"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOT36469.1"
                     /translation="MRTWRHRFLLVSMIVIAAWLPAAAVYLPLDNRAYEDIDALALRG
                     YRVVPATTIKPYSRKTVAQGLSHLSDRRADMSAAEREMYDRLLAEFADNIADEAASPG
                     PSSGDRWWLKADVNPRAMAQTTNERTDLPRARRVPATRRTLNDRARLIGTLDLTFRLT
                     RNLSTFQRLEMDTDGLWDNDFRGHRVYGEDWGMFNATGQLEEAYITYDNDWGSVGFGR
                     IQRMWGPERQGSIFLSDNSPAMDMLSADVHLNWLHFASITAELEPEQDASGTPISRYY
                     AGHRLVLTPFRWAEIGLSESVIYGGTNQGFSLRWSNPFVFLYGEEVNISPANENLLVG
                     ADISLRPVRNTEFYGAFLIDDVSIDKPKLAPDRIAWSAGARWEAPFGIDRLGAGAEYV
                     RLSRWVYNYARIAPWRHYVNKNAVLGSFLGPDGDALFLEGRWVTQNGTAIKGLLAHRR
                     QGETRYNTPTPNYLPSDYRYPCEPFPFGIVEKRTSLEAIVEFPPVYGASVAGALSGST
                     TLNRDNKKAPRHWGWGLRLDLDWHIPVRW"
     gene            complement(6175..7440)
                     /locus_tag="PLT86_07385"
     CDS             complement(6175..7440)
                     /locus_tag="PLT86_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36470.1"
                     /translation="MTRNPAARIWVRTMLAFLLFGFARATCAEQGLPGDSGCTYHSVT
                     FSLIPRVSTTYGHSKPVTNLSINLLVGKHFALRGVEIGGITNVQESYARGLQVAGIVN
                     VAGGNVRGIQSAGIVNVAGQTVRGIQGAGIINIAGKNLIGTQASLIGCISGNDVRGSQ
                     TAGIFAIAGRSVEGFQGSYVIGISGAGTIGAQTSGIMSISGGGVEGVQFAGIGSIAGG
                     TVEGIQAAGIFAVAPQVRGAQFGMFTAADSVFGLQVGFVNRSKRAEGYPVGLINCVEG
                     VPVKVGFVVDESAGSYVELTTGSLHTHGIVALGGRILAAPQTQAVGLGVGLHFQKTRH
                     YWDIDAITYGNFAADSAGEGAVSALRFILGIPVNRHLDLFAGGRVSLFVSDTGTGAAL
                     APWSLYKGTVRGRYVRVWPGLTGGVRVSL"
     gene            complement(7437..8882)
                     /locus_tag="PLT86_07390"
     CDS             complement(7437..8882)
                     /locus_tag="PLT86_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOT36471.1"
                     /translation="MSGFFSSTAGRRLVLYGLVVLVLALGTAGVLLLLENISQRKYEA
                     RQVFFRVVELNEDTEDPSVWGKNFPNQYDTYLRTVDTVRTKYGGSEAFQKLDAFPVWR
                     ELFRGYAFGIDYREERGHAYMLSDQRQTERVTKKPQPGACVQCHASVIPAYKKVGVSQ
                     GGVPDDDAHRADAIMAGFELVNAMPYSEATQLVKHPVTCLDCHNPATMQLRVTRPGFL
                     VGIQRLAESGVSLPHLPSIERWRRGDRSKPYDANTLASHQEMRAFVCGQCHVEYYFKP
                     PNTQLTYPWHNGLRVEHIEAYYDSVGWKDWTHATSGAPVLKAQHPEFETWSQGIHARA
                     GVTCADCHMPYIRVGAVKISDHQVRSPLLGINRACQGCHRAPETELLARAITIQDRTS
                     ALMTRAENATLALVLAIRAAQERGASDEQLTKPRDMHRKAQWRLDFVAAENSMGFHAD
                     QETARILAEAIDYARQGETEVARIGSGRAPR"
     gene            complement(8914..9378)
                     /gene="nrfH"
                     /locus_tag="PLT86_07395"
     CDS             complement(8914..9378)
                     /gene="nrfH"
                     /locus_tag="PLT86_07395"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOT36472.1"
                     /translation="MDYTRTARWMSVFAVVLLGIGIGAGAYTLVYARGASYLLDDPQA
                     CVNCHIMREQFDGWEKSSHHAVATCNDCHVAHGPVSKWLTKAENGFHHSVAFTFQNFH
                     EPIQIRESSSLLLQSNCVRCHADITDAITWNHPKTRDRCVRCHDSVGHGPSR"
     gene            complement(9446..10657)
                     /locus_tag="PLT86_07400"
     CDS             complement(9446..10657)
                     /locus_tag="PLT86_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOT36473.1"
                     /translation="MATQGENPADDQVGAVLESLGLIVQDQRRINILLSRLVHDMDKR
                     VQALEEGGGIAAGRGARASVQIGKDIPAYPPSDFEFLKRTTVFGSLSDDALRVLLHKG
                     EVKTVPKGYVVFETGKPADTLWVIKKGVVEISRVVDGALKTVAYFTDSETIGEMRIIT
                     RSPHRSNGRAPGGGEIFFLTRDQLLECMELVPEIALRMCEMYAHKLEGTVTTLRTHEQ
                     KQRQLEGNLEYFDLATVIQTLLSTDQRTGILFVSDDKQQPVAELFIDQGHVKRASVGA
                     LTGEEAFYQLFLRELKRGNFFFKEDVDPAAETAEIDIPGMSLLMEAARLQDELSAIKS
                     EVITDPDKVYRQTSRTFEWQDDETKALAEVVWRAVGNGLSVRQIVEEIPRNEYTVYSL
                     LQQLIQNGQIG"
     gene            10905..12248
                     /gene="hisS"
                     /locus_tag="PLT86_07405"
     CDS             10905..12248
                     /gene="hisS"
                     /locus_tag="PLT86_07405"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT36474.1"
                     /translation="MASALIEPRVLRGFRDYLPEEMIPRQRMINTITAVFERHGFAPL
                     GTPALEYSEVLLGKYGADAEKLLFRFTDNGGRDVSLRYDLTLSLARVAAQYGNLPVPF
                     KRYQIAPVWRAERPGHGRFREFYQCDVDIVGAAGPLAEYECIQVDYDVMTALGIEKFS
                     IHVSNRKLLEGLSSKLGVSDSAQSRAIARTIDKLPSQGEGAVRALLFSDAGLNSEQIG
                     IVFEYLSLKGSNEAILAQLREFFANTPEAHSGIESLEAIIRCASAAGIPEGTIELDLS
                     IARGLDYYTGTVYETFLLDLPALGSVMSGGRYDGLVAIFTGQDVPAVGISVGIDRLFS
                     GLQETGRIPQAATVSQVLVTVFNESTTPQSLQAATRLRSLGVAAEVAVEGGALRRQLK
                     YANKQHIPFALIIGPDEAENGTMTLRDMESGEQESFSTVEAVAARVLEQRDDHLS"
     gene            12261..>12526
                     /locus_tag="PLT86_07410"
     CDS             12261..>12526
                     /locus_tag="PLT86_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36475.1"
                     /translation="MPRGQYCGTERIQRLDRVTGIPVVQVTSMFTPSVHMHYETVTFT
                     PDNSWMIFLSARSTGTGAPMNLVRASSDGKDIVQLSGDSAVSNVV"
BASE COUNT         2574 a         3795 c         3573 g         2584 t
ORIGIN      
        1 gatgtaaggg attgtgcacg ccgcgttcca gaggacaaaa atcgcgagaa gcggaaacag
       61 cggcgctgtc accatcgctc caagcatgct cgggtacctc agaacggcaa gaacaccgag
      121 tgcaagcgcc atctcgccta cgtatagcgg aggaaacccg aggtaggcaa agggtttccc
      181 gaacaccgcg taccccagca ggaccaccga cagcgcgttg agaaaccact tgccttcctg
      241 tttacgtttc ggcgcgggcg gaacaatggc gggttcggct acgggcggca tcggccggac
      301 ccggggcgct gttctggtcc ttggagccgc gaccggtgtc gtcatgtcgt caccaccagc
      361 ccatcatgcg cgatcgtatg gttgtacacg cggccctcgg gtgaccggtt ttccagacgc
      421 tcgcgtagct ccgccgccgc ggcgcgtgat ttcgccaacg cgatcagaaa gccgccgccg
      481 ccggcgccga ccagtttcgc tccgctacaa aggtgggaaa tatcgtccat gaggtcgttt
      541 atcgccgggg aactgctgcc tgggttcaac acgttgttca accgccacgc gcgattcatc
      601 agcgcgccta ttctgtcaat atcgccattt cgcattgcgt cccacaaatc cagcgccacc
      661 tgatgcaggt cctgcagcgc cgtcaccact tcctgatcgc gcgaaagcca cctgcccaca
      721 accccgcgca ggatgttttt cgcgaggcgt tgagccccgg tgtagtacac cacaaggcga
      781 tcctcaaatt catggagcgc ctgtgaatcg gcctcaatcg ttctgatcga gggtttctgc
      841 acgacgcctg gcagcgtttc gatgagcttg aacccgccta cggcgccgcc gatttggtcc
      901 tgccagccac caccggtggt aagcatctgt tccaggcaga ggacatcctg aaacaggtcg
      961 tccatctgac gcgtgattcc gcaaattttg ttgagcgccg ccaccagacc gattgccaga
     1021 aggctgctgg tacccagacc ggagcccttc ggcagatcac acgcgctttc cacggcgatg
     1081 ccggccccga acgcctgcaa atacggttct agcggccctt ctgcccctgt cgggatgatc
     1141 cccagtaaca cgagcgctgc cttgtgcagt gcgacggggt ctccggggtc gttgtagctc
     1201 acaatcggct cgcaacgtgt gattcgcgcc gaataaccaa tgtcagtgga ggtcagcact
     1261 accacaggct cgaggattgg atacgccgtg acacggatgg gtcgcttccc attcaacaac
     1321 agcgccacat tcgcaaccac gccacccttt tccaggctgt acggcggcgt gtcagtccac
     1381 ccgccaccca ggtcgattcg gacaggtacc tccgcaacga cccttgttcc tggggaaacc
     1441 gccagatgcg gctgagcacc cctacgcagg ctgtggcttt ctatgacctg tgcgacctgc
     1501 aggaaggcca gttcttcgtg taccctggcc aacgtcgcca gcgaacctgc ttcctctgtg
     1561 tctgtcgccc actgatgcac gtaaggggcc gtgaattcac gccaggcgcc gagcgccccg
     1621 gacggaattg acacggctcc agaatccaca tgtgcatgcg cggaaatcga tcgcagccgt
     1681 tcgttgaaaa ggtcactcgc tgccttgaac gctcgtgcct tcagaaacgg ggaaggagcg
     1741 gtttcagcga aggcacggat ctcattggcc gcggaagcgc aaagcgcgcg cgtatcggcc
     1801 gccccgaaga gcggacggac gtcgttgtca gtacccagcc gttcgcgaac ctgcgcgcac
     1861 agggctgcag ttctgattgc gtcgcggttg gaaatgattc tcccgcagtc cgcttttttg
     1921 aaggcttccg ccatcgacaa gcgtcggacg gttttccatc gttcctgctc ccgtttggtt
     1981 ggcgggcgga gcggcatcca aaggaattcc gtgatgtctt ctagggttga gagcacaggg
     2041 aacagccgcg cgtcccagat cgtctgctgg tcgccgacgt cccagagatc ggtcctgttg
     2101 atgccggcag catccatcca actggacatg gcggtattgc agaaggtgca gccagcttcg
     2161 tccgcggaaa gcttcggatt gtctttcaga ccgtacagga ggatggcgta gtctcccgcc
     2221 tcgtcatccg ccagagggat taccgcgcag acgcattcgt ccccgagaga gatgtcttga
     2281 tccgggctga cgagaccgct gaggatagaa cgcctcccga cggtcgcgcg gcgaaggtgg
     2341 cacccgatca acacggcatc ttcgccgatt acgctcgtat tatcgcgcag aacagtttca
     2401 ataaggcagc ttttcgcggg gggtgtggtt ccacttgcat acaccgatct tgcgaccgca
     2461 tacccgtatc catggttttc cgcttcacgc gggacatcga ggtactgctc cgttgtaccg
     2521 atgtggacaa atcggatggg tgataccgtt acgtggtcga aatggaactg atgcagagaa
     2581 ttccagatcg tttcccgtat ctgacaagcc ccgccaccgg cgacgtagac ggcatgatca
     2641 gtttcgcgag cgagggcgaa cgcgaaatcc tcgtacaatt cgatcctcga accgaggctg
     2701 cgtgccgacc tcaccaaatc cgcaccgcca tgcagaccgg caaggcgcgc acatgcggcc
     2761 gttgcagatt cgtcgaaaag aaggatcccc gtatcggcct tgacttcccc cgaggaaccg
     2821 aatgcgcccg acacttcaag cgcttgatga tcgggtttct gcagaaacaa tctcaccgaa
     2881 ccatcctgtg ccgcgacaaa caccccgaaa tgcgctgccg tgtcttccaa ggcattgaag
     2941 gccacgccgc gaacaccacg aagttcgttc gcgcggatag cgcggtggtc gaaaaccagc
     3001 agtacatctc cggagaggac catcattccc gcggaaaggt ctcctgcgca accggctact
     3061 gccacgaaca actggtcaaa aggagtgatc ggctggcctt ccgggtcgag cagcgggaga
     3121 acaccgaacg ctttccccgt ggccgcgaga tgaggaaccc gcttactgtc cccgcccgaa
     3181 tgaatgatga gaatccgggt tttgctccat gcggattcgt ccgcgacttc accgcgggcc
     3241 gacttgtcct cgaaaatggc ccggagagcg ttcagtgtcg cgcctccgct cccgatgcgt
     3301 tgtccgtcag ggtcgggaac cacgaggaag cgagtggaag gcggcaattt gcctctcagg
     3361 cgccgccagt caagctcaga ctcgtacgca cgcgcctgtc gctcattggc cgacgtgatc
     3421 acacaataat cccacgcagg ggcaagccct tggattacct tctggtacgc cgaccagttg
     3481 gcgcgtaccg cggcctcgaa cagacgcgtt tcagaatcag ccatttgtgc cctcagaaag
     3541 gccgatcgct ccgattggag cgaattgcct caccccgaga acctgcaaca ggaaaaaaaa
     3601 cacgaaacga gggttgtgct tgagataccg tttccagagc cggcgaggtt cggttgccaa
     3661 ccgaaacaac cattccatac ccattttctg gaccaggcga ggcgcctgag gtttcacgcc
     3721 ggcgaggaaa tcaaacgcag caccgacgcc caccatcacc gcaggcaacc gaccgcgatg
     3781 ctcgtgcatc caccgttcct gtttgggaca tcccagtccg acaaacaaga cgcgagcacc
     3841 agaatccgta atctccctga cgattgtttc atcctcatcc ggcgaaaggg ggtggaacgg
     3901 cggggcgtat gcgtaggtga tcaccaggtg cgggaaacgc tcccggacaa tcgacacgag
     3961 tcggtcgagc gctgcctgag ttccaccgcc gaagaagccg accggtatcc tgtgatcagc
     4021 cgcctttttg agaagttgtg gtgtgagttc cggaccgtac acgcgtgtaa caccctttat
     4081 tcccaaccac ctgagtgccc agacaagagg cacgccgtct ggcgtaacca ggtcggcgct
     4141 gttgaccacg tgttggtacg cgggcgagtc gtggccttcc attaccatgt gcacggtgct
     4201 tacgcagacg taccgcgatt cgccctcccg tgcccacgat atgcaccgat cgacggcatc
     4261 ttcgtagctt gtcgcatcaa ctcgtgttcc cacaatgaag cggctttcga gggccttttg
     4321 cggcatttgt gcaagccggg ttgtcgactg tgcgttcatt ggcgcgcgtg aagttttgag
     4381 aaaagaaaac gaggaatacg aacgaattcg tatcgcaaaa tatacgacga gatacgacca
     4441 tcgacggtga caaaacacac aaaacgcggg gagagatagt tagagttgtt cttgtcgctg
     4501 cagtttcaga aattcaatcg atggttcgac ttttgcgatt ggagcgcgtg caccatgcgg
     4561 acatggcgac acagatttct gctggtatcc atgatcgtaa tcgcggcatg gttgccagcg
     4621 gccgcagtgt accttcctct cgacaaccgc gcctacgaag atattgacgc gcttgccctg
     4681 cgtgggtacc gcgtcgtgcc ggcgacaacg atcaagccgt attcgcgaaa aacggttgca
     4741 caaggccttt cacatctctc ggaccgccgg gctgatatga gcgccgcgga acgggagatg
     4801 tacgatcgct tgctggcgga atttgcggat aacatcgccg atgaagcggc aagtcccggc
     4861 ccgtcatccg gggatcgttg gtggctcaag gcggatgtta acccacgggc gatggcgcaa
     4921 accactaacg aacgaacaga cctgccacgc gcccgtcgcg tgccggctac gagacgcact
     4981 ctgaatgaca gggcgcgcct gattggaacg cttgacctga cgttccggct tacgcgcaac
     5041 ctgagcacgt tccagcggct ggaaatggat acggatgggc tttgggacaa tgacttccgc
     5101 gggcatcgcg tgtacggtga agactggggg atgttcaatg ccacaggtca gctcgaggaa
     5161 gcgtacatca cgtacgacaa cgattgggga agcgtagggt tcggccgaat tcagcggatg
     5221 tggggaccgg aacggcaggg atcaatcttt ctctctgaca attccccggc gatggacatg
     5281 ctatcggcgg acgtgcacct caactggttg cattttgctt cgattaccgc ggagttggag
     5341 ccggagcagg atgcgtcggg aactccgatc agtcggtact acgcaggtca ccgccttgtg
     5401 ctgaccccgt tccgttgggc ggaaattggc ctttcggaat ccgtcatata tggaggcacg
     5461 aaccagggct tctcgcttcg atggtccaac ccgttcgtgt tcctgtacgg cgaagaagta
     5521 aacatcagcc cggccaacga gaatctcctt gtaggtgccg acatttcctt gcgcccggtc
     5581 cggaacacgg agttctacgg ggcatttctc atcgatgatg tctcgattga caagccgaaa
     5641 ttggcgccgg atcgaatcgc atggtctgca ggtgcacgct gggaagcgcc gttcggcatt
     5701 gacaggctgg gtgccggtgc agaatacgtg aggctgtcgc gttgggtgta caactacgcg
     5761 cgcatcgctc cgtggcgcca ttatgtgaac aaaaacgcag tactggggag ctttctcggg
     5821 cccgatggcg atgcgctgtt ccttgaggga cgctgggtaa cgcaaaacgg gactgccatc
     5881 aaagggctcc ttgcgcatcg gagacagggt gagacgcggt acaacacgcc gacgcccaac
     5941 tacctgccca gcgactaccg ttacccgtgc gaacccttcc cgttcggcat cgtggaaaag
     6001 cgcacgtccc ttgaggcgat tgtcgagttc cccccagtct acggtgccag cgttgctggc
     6061 gctctttctg gaagtacgac tctgaacagg gacaacaaaa aagcccctcg ccactggggc
     6121 tgggggctgc gcctcgatct tgactggcac atacctgtgc gatggtgact cccctcaaag
     6181 actcacgcgc acgccgccgg tcaatccggg ccagactcgt acatacctgc ctcgcaccgt
     6241 ccctttgtac aggctccatg gagcaagcgc tgccccggtt ccggtgtccg aaacgaacag
     6301 gctcaccctg cctccggcga acaggtccag gtgccggttc accggtattc ccagaatgaa
     6361 ccgcaacgcc gaaaccgctc cctccccagc ggaatctgcg gcgaaattgc cgtacgtgat
     6421 cgcgtcaata tcccagtaat gccgtgtctt ctggaagtgc aaccccaccc ccaaccctac
     6481 tgcctgggtc tgtggcgcgg cgagaattcg tccccccaga gcgacgattc cgtgagtgtg
     6541 caggcttcct gtggtcaatt cgacgtaact tcctgcactc tcgtcgacaa cgaaacctac
     6601 cttgaccgga acaccttcca cgcagttgat caggcccacc gggtaccctt ccgcccgctt
     6661 ggatcggttc acaaatccca cctgaagccc aaagacggaa tccgccgcgg tgaacatgcc
     6721 gaactgcgca ccgcgcacct gaggagccac cgcgaaaatg ccggctgcct ggataccttc
     6781 aacggtaccc cccgcgatgg aaccgatacc ggcgaactgc acgccctcaa cgccgccacc
     6841 cgaaatactc ataattccgc ttgtttgagc gccgatggta ccggcgccgg aaatgccgat
     6901 gacataactg ccctgaaacc cttccacact ccgccctgca atagcgaaga tacctgccgt
     6961 ctgggatcct cgcacgtcgt tgcccgagat gcagccgatg agcgaagcct gtgttccgat
     7021 gaggttcttt cccgcgatgt tgattattcc cgctccttgg attccccgga cggtttgccc
     7081 ggccacattc acgattccgg cagattgtat gcctctcacg ttcccgcctg ctacgttgac
     7141 tataccggca acttggagcc cgcgggcgta ggactcctga acgttcgtga tgccgccgat
     7201 ttccaccccg cgcaacgcga aatgcttccc caccaggagg ttaatggaga ggtttgttac
     7261 cggtttgctg tgtccgtacg tggtcgagac acgggggatc agcgagaagg ttacggagtg
     7321 atacgtgcag ccgctgtcgc ccggcaaacc ttgttccgcg cacgtcgccc gcgcgaagcc
     7381 gaacaaaagg aatgccaaca tcgtgcggac ccagatccgg gccgcgggat tcctcgtcat
     7441 cggggtgccc ttccagaacc gatgcgcgcg acctcggttt ccccctgtct cgcgtagtca
     7501 atcgcctcgg ccagtattcg ggcggtttcc tggtcagcat ggaaccccat gctgttctcc
     7561 gccgcaacga aatccagtct ccactgcgcc ttccggtgca tatcgcgcgg ctttgtcagt
     7621 tgctcgtcgc tggcccctcg ttcctgcgcc gccctgatcg caaggacgag tgcgagcgtg
     7681 gcattctctg cacgcgtcat cagcgcgctc gtccgatctt gaatcgtaat tgcacgtgca
     7741 agcaattcag tttccggcgc gcggtggcag ccctgacagg cccgattgat tccaaggagc
     7801 gggctccgga cctggtggtc actgatcttc actgccccta cgcggatata cggcatatgg
     7861 caatcagcgc aggttacccc tgcacgcgca tggattccct ggctccacgt ttcaaattcc
     7921 ggatgttggg ccttcaacac cggtgcaccc gaagtcgcgt gtgtccagtc tttccaaccc
     7981 accgaatcgt aatacgcttc gatatgttcg acccggaggc cgttgtgcca gggatatgtg
     8041 agctgagtat tcgggggttt gaagtagtat tcaacgtggc actgcccaca cacgaaggcg
     8101 cgcatctcct gatgagatgc cagagtgttt gcgtcatacg gtttgctgcg gtcgcctctt
     8161 cgccagcgtt ctatgctggg cagatgtggc agggagactc cggactcggc aaggcgctga
     8221 attccgacga gaaaccccgg gcgcgtaacg cgaagctgca tggtcgccgg gttgtggcaa
     8281 tctaggcacg taaccgggtg cttgacgagt tgcgttgcct cagagtaagg catagcgttg
     8341 accagttcga accccgccat gattgcgtcc gcccgatgag catcgtcatc gggtacgcca
     8401 ccctgcgaca ccccgacttt cttgtaggcg ggaatgacgg aagcatgaca ctgaacacac
     8461 gcgccgggtt gcggtttttt cgtgacgcgc tccgtctgtc gttggtcgga aagcatatac
     8521 gcatgcccgc gttcctcgcg gtagtcaatg ccgaaagcgt atccccgaaa aagttctcgc
     8581 cacacaggaa atgcgtccag tttctgaaaa gcctcgctgc cgccgtattt ggttcgcacc
     8641 gtatcgacgg tccgcaaata cgtgtcgtac tggtttggaa agttctttcc ccatactgac
     8701 gggtcttcgg tgtcttcatt gagctcaacc acacggaaaa aaacctgtcg tgcctcgtac
     8761 ttcctctggg aaatgttctc caagaggagc agcaccccgg cagttccgag cgcaaggaca
     8821 agtacgacaa gcccgtacag cacaagtcgc cttccggcag tggaagagaa gaagccgctc
     8881 atgacatccc tcactgactg gggttacaga agcttatcga ctcggcccat ggcctacaga
     8941 atcgtggcaa cggacgcagc gatcgcgagt tttcggatgg ttccacgtga tagcgtctgt
     9001 gatgtcggcg tggcagcgca cgcaattgct ctggagcagg agcgaactgg attcgcggat
     9061 ctggatgggc tcgtggaaat tctggaaagt gaaggctacg gagtggtgga acccgttttc
     9121 tgccttcgtg agccattttg agacgggccc atgcgcgaca tggcagtcat tgcaggtagc
     9181 cacggcatga tgagaggact tctcccaccc gtcgaactgc tcgcgcatta tgtggcagtt
     9241 gacacaggct tgcgggtcgt cgaggagata cgacgcacct ctggcatata ccagagtgta
     9301 cgcccccgcc ccgataccta ttccgagcaa tacgactgca aagacagaca tccagcgagc
     9361 agtgcgcgtg taatccatac ctgcatccga agggttgcgg acacaccgag cgggtacgca
     9421 ggtcgtcctg gtgaaatgac gaatgtcaac cgatctgccc gttctggatc aactgttgca
     9481 acaggctgta caccgtgtac tcgttgcgag gaatttcctc tacgatctgc ctcacgctga
     9541 gaccgtttcc gaccgctctc cagacgactt ccgccagcgc tttcgtctcg tcatcctgcc
     9601 actcaaacgt gcgggaagtc tgtcggtaca ctttgtccgg atcggtaatg acctccgatt
     9661 tgatcgcact caactcgtcc tgcaatcgtg cggcttccat cagaaggctc atgccgggga
     9721 tatcgatttc cgccgtttcg gcagccgggt caacgtcctc cttgaagaag aagttgccgc
     9781 gtttcagctc cctcaggaaa agctggtaga atgcttcctc ccccgtcaaa gctcccaccg
     9841 aggctcgttt gacgtgtccc tggtcgatga acaactcggc aactggttgc tgcttgtcat
     9901 cgctcacgaa gagtatgccg gtgcgctggt cggtgctgag cagagtctga atgacggttg
     9961 cgaggtcgaa gtactccaga ttgccctcaa gttgccgctg tttctgctcg tgcgtgcgga
    10021 gcgtggttac tgtgccttcc agcttgtggg catacatctc gcacatgcga agtgcgatct
    10081 ccggaacaag ctccatgcat tcgaggagtt gatcgcgtgt caggaaaaag atctccccgc
    10141 cgccgggggc ccggccgttc gaacggtgcg gggatcttgt aataatgcgc atctcgccga
    10201 ttgtctcaga atcggtgaaa taggcgacag tcttcagggc cccgtcaaca accctgctta
    10261 tttcgaccac gccttttttt atcacccaga gggtgtctgc gggtttgcct gtttcgaaaa
    10321 caacgtaccc cttcggcacc gtcttcacct cacccttgtg aagcagcacc cgtaacgcat
    10381 cgtcgctcaa tgacccgaat acagtagttc gcttgagaaa ctcgaaatcg ctcggtggat
    10441 acgccggaat gtcttttcct atctgcacac tggcacgcgc gccccgccct gcggcgatcc
    10501 ctccgccttc ttccagcgcc tgcacgcgtt tgtccatatc gtgcaccagg cgcgacagga
    10561 ggatgttgat ccgccgctgg tcctgaacga tgaggcccag ggattcgagc acagcaccaa
    10621 cctgatcgtc cgctgggttt tccccttgag tcgccatggt gtacttcctc cgtgacgttt
    10681 ccgtgtacgt cgggttctga actgaccggt gcgagaacct cccttttctg caatccagag
    10741 ccaacaatac aaaaacagcc accgggatca aagtgcggcg gtccagactc gggaaaggtc
    10801 tgacgcgggt ctgtggcgga ttgccccgat gcgcgcgggg aacgattttt aggttcgcac
    10861 gtattcgcct ttacaagttg cagtcaaccg gagcaagcat acctatggcg tctgcattga
    10921 tcgagcctcg cgtgttgaga ggattccgcg actatctccc tgaagaaatg atcccccgtc
    10981 aacgcatgat caacaccatc acggcggtgt tcgaacggca cggttttgcg ccactcggca
    11041 ctcccgcgct tgagtactct gaggtactcc tcggtaaata cggtgcagat gccgagaagc
    11101 tcctcttccg cttcaccgac aacggcgggc gagacgtaag cctgaggtat gacctgacgc
    11161 tctcgttggc tcgcgtggcg gcgcagtacg gtaacctgcc cgtgcctttc aagcggtatc
    11221 agatagcccc ggtctggcgc gcggaacgcc ccggtcatgg tcgcttcagg gagttctatc
    11281 aatgcgacgt ggacatcgtc ggtgcagccg gtccgctcgc ggaatacgag tgcattcagg
    11341 ttgattacga cgtaatgacg gcgctcggta tcgagaaatt ctccatccat gtctccaaca
    11401 ggaaattgct ggaagggctc tcgtcgaaac tcggggtttc cgatagcgcg cagagtcgcg
    11461 ccattgcccg taccattgac aaattgccct cgcaggggga gggagcagtt cgtgccctcc
    11521 tgttttcgga tgcgggactg aattcggaac agatcgggat cgtattcgag tatctctcgc
    11581 tgaaaggaag caacgaggct attttggctc aactgcgaga gttcttcgcg aataccccgg
    11641 aagcgcattc cggcattgag tccctcgagg cgataatccg ctgcgccagc gcagccggaa
    11701 taccggaggg caccatcgaa ctggacctga gcatagcccg tgggcttgat tactacacgg
    11761 gcaccgtgta tgagacgttc ttgctcgatc tccccgcctt ggggagcgtc atgagcggcg
    11821 ggcgttatga tggcctcgtc gcgatcttca cgggccagga cgtcccggcc gtcggaattt
    11881 cagtcggaat tgaccgcctc ttctcagggc tccaggagac tggcagaatt cctcaggcgg
    11941 caacggtttc gcaggtgctg gttacggtat tcaacgagtc cacaacaccg caatctctgc
    12001 aggctgcgac ccggctccgt tccctcggtg tggcggcaga ggtggcggtc gaagggggag
    12061 cactgcgcag gcaactcaaa tacgcgaaca agcagcacat accctttgcg ctgataatcg
    12121 gccctgacga agcggaaaac gggacaatga cgctccggga catggaaagt ggcgagcagg
    12181 aatccttttc gaccgtcgaa gccgtcgcag ctcgtgttct cgaacagcgc gacgatcatc
    12241 tctcctgacg gagttcctcc atgccacgcg gccaatactg cggtaccgag cgaatccaaa
    12301 gactggatcg tgtgaccggg attccggtgg tccaggtcac cagcatgttc accccaagtg
    12361 tgcacatgca ctatgaaacg gttacgttca caccggacaa cagctggatg atcttccttt
    12421 ctgcccgttc caccggaacc ggcgcgccga tgaaccttgt ccgtgcgagt tcggacggaa
    12481 aagatatagt gcagctatcc ggggattccg cggtatcgaa tgttgt
//
LOCUS       DAOSOO010000081        12251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5169_length_12251_cov_5.224090, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000081 DAOSOO010000000
VERSION     DAOSOO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12251
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5169_length_12251_cov_5.224090"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /gene="rpsA"
                     /locus_tag="PLT86_07415"
     CDS             complement(<1..1726)
                     /gene="rpsA"
                     /locus_tag="PLT86_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOT36476.1"
                     /translation="MAEEQIIPSAAEPAPVEQPAADKAPVQATKEERTPARVKFVSDV
                     NELPRDDEYASEEYEQLISKYESSFAVKEGEIVKGKVLRIENDEVIVDIGFKSEGSIK
                     LAEFTDPSEAQVGNEIEVFVEEIEDQEGQVVVSKQKADFMRVWDRIKEAFDSGQPVAG
                     TLTKRIKGGIVVDLFGVDAFLPGSQIDLRQVKNFEQYLGKTFDFRIIKLNKNRRNIVV
                     SRRVLLEEERETMRDELLKTLEKDQIREGLVKNITDFGAFIDLGGVDGLLHITDMSWG
                     RINHPSEVVKIGDHIRVKVLNYDKERGRISLGLKQLAQYPWKGVQERYPVSSRVKGRV
                     VSITDYGAFVELEEGVEGLIHVSEMSWTQHVRHPSKVLNIGDEVEALVLRVDEANEKI
                     SLGLKQTQPDPWETLDERYPVGTKLIGKVRNITNFGAFVEIEEGIDGLVHISDLSWTK
                     RIKHPSEVVKKGDDLPVVVLNIDKEQRRISLSHKHCEENPWDSLEAQYQPGVQTSGVI
                     SRVTDKGVVVDLEGDVEGFIPVSQLGIEDMRTPEEHFSPGETLNMVVVEFDREQKRIV
                     LSVKRYLEH"
     gene            complement(1839..2492)
                     /locus_tag="PLT86_07420"
     CDS             complement(1839..2492)
                     /locus_tag="PLT86_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT36477.1"
                     /translation="MTNQPSPYSFWSRVLYRIIWSIIRFFLVVAFRIKVSGRENERMH
                     GRVIYASNHISALDPPVVGASLKRQLMYLAKQELFAIPIFGRIIRALHARPVNRAGYT
                     RGALEVMKAALEKDEGVLVFPEGTRQKDGRLGEGKVGVGMLAVWTGAPVVPVYVSGTA
                     NVWKALSGRCRFRVALGRVVEPPDVSDPQERKRAYQTVTDAVMAEIARLKAEVDEKQ"
     gene            complement(2489..3166)
                     /gene="cmk"
                     /locus_tag="PLT86_07425"
     CDS             complement(2489..3166)
                     /gene="cmk"
                     /locus_tag="PLT86_07425"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT36478.1"
                     /translation="MVVAIDGPASSGKSTTAREVAKVLGFTHIDTGAMYRALTLAALR
                     RNVRLEDDEELTRLAREVRIELGQENGVPWVKLDGEDVSQAIRTPEVDRHVSRVSEVA
                     GVREEIVRQQRVLAGSRDVVMEGRDIGTVVFPNAEVKVFLVADPVTRARRRKAELEQR
                     GIVRPLEEVLSEIVRRDEYDSSRAVSPLKRAEGAEDLDTSELSIQQQVDEVVARVRRY
                     LEKVHTT"
     gene            complement(3150..3911)
                     /locus_tag="PLT86_07430"
     CDS             complement(3150..3911)
                     /locus_tag="PLT86_07430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT36479.1"
                     /translation="MNAPADNACVVRVNVFLSRCGVASRRSADKLIASSRVTVNGLPA
                     ELGARIDTTQDVVCLDGRVITPVESKTTLVMNKPSGYLVSRRDPHYTRTVYDLLPSEY
                     AHLVPVGRLDLDTEGVLLMTNDGGLVEHLTHPRYRIPRVYRALVKGHPDAQALDRLRN
                     GVEIEGGTTSPAEVRVCEKRKDTSELELVLREGRKREVRLMCRAVGHEVLRLKRTEFA
                     GIGLAGLATAAWRVLSAAECERLWKQVECVDGRGD"
     gene            complement(3992..6706)
                     /locus_tag="PLT86_07435"
     CDS             complement(3992..6706)
                     /locus_tag="PLT86_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT36480.1"
                     /translation="MSFSTRGWLLASRSMDLLRQEIELAPRFGINHVQLSHSFCAYAE
                     QIVESSELQRDAAELIERAHRHGIEVFVWTHEMQNVPDHLKLGGKVDLNSRRTWDYVA
                     QKYEDLFSAVPELDGVVLTLTETRIRIDDDNEVVSDRPQPHRIARMVSMLDAICRKHK
                     ARLIVRTFTWVPRTMLWYAEAMRDVPDHVTIMTKESWGDWYQAQPPNPLLGLFGRHPQ
                     MIEFDCWGEYAGGTDFPWVSYEHTRNRLRYAASLGLTGAIARVDRAERSTFGSLNEFN
                     TIAFSELAKNLDKDLESLWRDWLTSKYPSDAVDAIGRALQRTNQIVQLTFYTKGILNV
                     CTSSPSLVEAEADSMIRFYRDFHGQWKTKLAVLADQLANPTEETLVQILWEKDRALSL
                     CERSIADIESIRSKLETAKYEILHTGFEKMRVQVRAWRAMTEVVYLHKILERDPTPRL
                     REWFERAGRNLERVAEDIEHVHGKGFSRVRPEPLRNVWRVAESIHSKSTAWSTPLGLY
                     VAASPAIGDLEGNGSLSVVAVSSHKEIVALDGNGRRMWTTTTRGERYEYPHFSSPLIT
                     DGRDGGGARVVVGAADGRLYCLDGQGRHVWEFATGNRVDSAPAAGDLNGDGIEEIVAA
                     SLDGTVSCLNLRGERLWNVDLLEPIFAAPVITPAREIIVVTLQGTIACLKADGGIRWR
                     QALVGGEIHRTSFQGVPSGKKYALAEGGPNAIYSSPLLCQLLGAGKPALVLGVVTGKV
                     LAFDLDGTLLWEATTAGRVFSSPAVLPGDGKKACVVIGSDDGRVHGIDCAGRLSWQFQ
                     TGGAVRSSPVVVPASIAGEARIVVGSDDNRVYVIDEEGREVDDYLTGAEIFGTPAIAD
                     VNGDGSLEIVVGSYDHRVYAFRTPWKAEKYSVISGTFRSTNTRTGCVT"
     gene            complement(6827..7624)
                     /locus_tag="PLT86_07440"
     CDS             complement(6827..7624)
                     /locus_tag="PLT86_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT36481.1"
                     /translation="MNKPVFGLSTAHIAHESVSQAIDTCCALELDGVEFFTREYTVAE
                     CREIRQMAQDAGLFVDYHAPWDGVYNFGQTPVDIAFDRLESAVARTHLMGGRHLVCHL
                     GLFDLAEKDGRNRALDRVLKLTERILPALEDTGVMLCWEDNTLCHDPNPLGDQPYDFA
                     FLFSAIQSPHVGMTLDTGHAHVTGNTRAYLEQFGNRVYYLHLNDNNGIDDQHVAPSSG
                     TIDWPELMQQICHYGAAPCFGIEFNEREVPLELPFLRSLAAPLPWRW"
     gene            complement(7659..8420)
                     /locus_tag="PLT86_07445"
     CDS             complement(7659..8420)
                     /locus_tag="PLT86_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT36482.1"
                     /translation="MNQREPQEPYIRALRREIEELTKQLSDMERNHQRAIGELKASLF
                     VSQVQTVDAIVEAIEAKDPYTSGHSRRVMELSLMIGAAMHLEQERMWRVHVGALLHDV
                     GKIGVLDQSLRKTTRLDDLEYLQLKAHPLIGERIVRKIDQFADVAPIVRWHHERYDGQ
                     GYPDGLKGDQIPVESAIIFVADAYDAITSKRTYNKPRTRVEAADEIQKHAGTQFHPDV
                     AAALRAALEPNRLESIHIDESDFQDGFDIPEDIGI"
     gene            8743..9180
                     /locus_tag="PLT86_07450"
     CDS             8743..9180
                     /locus_tag="PLT86_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT36483.1"
                     /translation="METIRVHSDFHAAHRQIGYAGKCRHVHGHTWRGTISLSTEKFPR
                     NHLDMAIDFGDLKQVFRRMDHKMLVGEADREFVTAQGVDPEGVVVIPGSNPSVENIAY
                     YCLDGVRKVIEHQYPGKGLKYHLELTLQETENNIFVVETDFVI"
     gene            9296..9697
                     /locus_tag="PLT86_07455"
     CDS             9296..9697
                     /locus_tag="PLT86_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36484.1"
                     /translation="MGFRALVGSVWCALLGISFAACGNNVAKLVSPLQTSGSVTIEIS
                     RSTTPVFSWNATTTIEAISVANFRVDGTVDRILWGYRGIAATPPITYGSVIPGGISLS
                     VGTPPALQSGNRYRVEVVSGGKASTADWIVP"
     gene            complement(9701..10795)
                     /gene="ugpC"
                     /locus_tag="PLT86_07460"
     CDS             complement(9701..10795)
                     /gene="ugpC"
                     /locus_tag="PLT86_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOT36485.1"
                     /translation="MATVVLKNVTKVFDQRVFAVRDANLEVADKEFVVLVGPSGCGKT
                     TTLRMIAGLEEISSGEIYIDGRLVNDIPPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKYPRTEIDARVREAAQILEIGELLERKPKALSGGQRQRVAVGRAIVRKPRVFLFDE
                     PLSNLDAKFRVAMRTEISKLHDRLGATMIYVTHDQVEAMTMGDRIVVMDAGTIQQIDT
                     PINLYDAPRNKFVAGFIGSPAMNFVEGIIEDGTGTVFNEGSFRLPVPVHLTSALHEYS
                     GKKVVLGIRPESLLLSNDKVASGSFDARVELVEPMGSETFVYARTANHALIARVDAHV
                     APPNGSTINLIPRPDKMHFFDAESGAAIRA"
     gene            complement(10843..11706)
                     /gene="accD"
                     /locus_tag="PLT86_07465"
     CDS             complement(10843..11706)
                     /gene="accD"
                     /locus_tag="PLT86_07465"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOT36486.1"
                     /translation="MGWLDAWKAGFARNAKKAIPDGVWVKCEGKGADAQLGCGKTLYR
                     RDLEENLWVCPSCGFHMRIGAHAYLDLLLDEGTAEPLFETVMSADPLQFKAEKRYGDQ
                     IMEDFRKTGRGSAFMAASGAIYGRPFAVGAMDFSFRGGSLGSAEGEKINRLVGFAQDR
                     RVPLVIVSQSGGARMQEAALSLMQMAKTSARLAEFAESSLPFISVLTSPTTGGVTASF
                     AMLGDVILAEPGALIGFAGARVRETIKEDLPPGFQRAEFLLEHGFVDRVVDRRSLKEE
                     VARLTKVLAPV"
     gene            complement(11693..>12251)
                     /locus_tag="PLT86_07470"
     CDS             complement(11693..>12251)
                     /locus_tag="PLT86_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36487.1"
                     /translation="GRLACALGCRWLLSANAAGGVASHLRVGDLVLAEDDIFLWNAKG
                     FRQHAGKRFSHIPRYSERLENAFDRAGSAVNVRFSKGVLAMMTGPCYETPAEVGMIRA
                     AGASVVSMSTLPESRWARLAGLDAAAVSCVTNLVPSSPLARIRHDEVLEVLEMSLGKA
                     ARLIERWLEDEYVRGASGRRCYGLA"
BASE COUNT         2568 a         3800 c         3176 g         2707 t
ORIGIN      
        1 ggtgctcaag gtaccgtttc acactcaaca cgattcgttt ctgttcgcga tcgaactcca
       61 cgaccaccat gttgagagtc tctccggggc tgaagtgctc ttcaggagtc ctcatgtcct
      121 cgatgccgag ctgactcacc ggaataaatc cttccacgtc gccctcgaga tcaaccacca
      181 cgcctttgtc cgttacacgg cttatgactc cggaggtctg gacgccgggc tgatactgag
      241 cctcaaggct atcccacggg ttctcttcac agtgcttgtg ggaaagggaa atcctgcgtt
      301 gctctttgtc gatattgaga accaccacgg gcaggtcatc gcctttcttg acgacctctg
      361 acgggtgttt gatccgtttc gtccatgaga gatccgatat atggaccaac ccgtcaatgc
      421 cttcttcgat ttcgacaaac gccccgaagt tggtgatgtt tcggactttg ccaatcaact
      481 tggtccccac tggatagcgc tcatccagcg tttcccacgg atcgggctgc gtctgtttga
      541 gcccgaggct gatcttctca ttcgcctcgt caacccgcag cacgagagct tctacttcat
      601 cgccaatatt gagaaccttc gaaggatggc gaacgtgctg cgtccacgac atctccgata
      661 cgtggatcag gccttcaacg ccctcttcaa gttcgacaaa agctccgtaa tccgtgatgg
      721 atacgacgcg ccccttcacg cggctgctca ccggataccg ctcctgcaca cccttccatg
      781 ggtactgcgc aagttgcttc aagccgaggg aaatccgacc gcgttccttg tcgtaattga
      841 gcaccttcac ccggatgtga tcgcctatct tcaccacttc cgacgggtgg tttatccgcc
      901 cccaggacat gtcggtgatg tgcagaaggc cgtccactcc gccaagatct atgaaggcac
      961 cgaaatcggt aatattcttg accaatcctt cgcgtatctg atccttctcg agagttttca
     1021 gcaactcgtc acgcattgtc tcgcgctctt cctcgagaag cacacgccgg ctgacaacaa
     1081 tgttccgccg gttcttgtta agctttatga tacggaagtc gaacgtcttg cctaggtatt
     1141 gttcgaagtt ctttacctga cgcaggtcaa tctgcgagcc cggcaggaag gcgtcaacac
     1201 cgaacagatc aacgacgatg ccacccttaa tccgtttcgt aagcgtccca gcaaccggtt
     1261 ggcctgagtc aaaggcttct ttgatccggt cccatacccg catgaaatcg gccttctgtt
     1321 tggaaaccac gacctggcct tcctggtcct cgatttcctc aacaaaaacc tcgatctcgt
     1381 tcccgacctg tgcttccgac gggtcggtga attcggcgag ctttattgat ccctcggatt
     1441 tgaaaccgat gtccacgatg acttcatcgt tctcgatccg caggacttta ccctttacga
     1501 tttcgccttc tttgacagcg aacgacgact cgtatttgct gatgagctgt tcatactcct
     1561 cggaagcata ctcgtcgtcc cgggggagct cgttcacgtc agaaacgaac ttgaccctgg
     1621 cgggtgttcg ctcttctttc gttgcctgaa cgggcgcctt gtccgcggcg ggttgttcca
     1681 cgggcgcagg ttccgctgca ctcgggatga tctgttcttc tgccattgtg gtttccttcc
     1741 cggacgctcg tatcccggtg gcgcgagcac ggtcgtcctg tctgcgcggc acgtcatcgc
     1801 aactgaagaa cgttgctgat gacgtggaag aatggtggtt actgcttctc gtctacctcg
     1861 gctttcaggc gagcgatttc cgccatcacg gcatccgtca ccgtctgata agcccgtttg
     1921 cgttcctgcg gatccgagac gtcaggcggt tccacgacgc gccccagcgc aacgcggaaa
     1981 cggcaacggc ccgaaagcgc cttccagaca ttcgcggtgc cactgacgta caccggaaca
     2041 accggtgccc ccgtccaaac cgcgagcatc ccgacaccca ctttgccttc cccgagtctg
     2101 ccgtctttct gacgggtccc ttcggggaac acaagcactc cttcatcctt ttcaagggcc
     2161 gctttcatca cctccagcgc gcctcgagtg tacccggcgc ggttcaccgg tcgcgcatgg
     2221 agggcacgga tgatgcggcc aaaaatcggg atcgcgaaaa gctcctgttt cgcgagatac
     2281 atgagctggc gttttaacga tgctcccaca acgggaggat ccaacgcgga aatgtgattg
     2341 gatgcatata tcacgcgtcc gtgcatccgc tcgttttcac gccctgatac cttgatacga
     2401 aatgccacca ccaggaaaaa cctgatgatt gaccatatga tccgataaag cacgcgtgac
     2461 cagaaagagt acggcgacgg ctggttcgtc acgttgtgtg caccttttcc agatatctgc
     2521 gcactcgtgc aactacttcg tcaacctgct gttgaatgga caactcagaa gtgtccagat
     2581 cctcggcgcc ctcggcgcgt ttcagcggag aaactgcgcg actcgaatca tactcatccc
     2641 gcctgacgat ttcggaaaga acctcttcca ggggtcttac aattcctcgc tgttccagtt
     2701 ccgcttttcg tctccgcgcc ctggtgaccg gatcagcgac aaggaagact ttcacttccg
     2761 cgttcggaaa aaccaccgta ccaatgtcac ggccttccat cactacgtca cgagagcctg
     2821 cgagaacgcg ttgctggcga acgatctctt cgcgaacccc cgcaacctcg ctcacgcgcg
     2881 atacatgccg atccacttcc ggcgtgcgga tcgcctgact tacgtcctcc ccgtcgagtt
     2941 tcacccacgg aacgccgttt tcctgcccga gttctatccg aacctcccgg gccagtctcg
     3001 tcaattcctc gtcgtcctcg aggcgaacgt ttcgacgcag agccgccagt gtcagcgcac
     3061 ggtacatcgc gccagtgtcg atatgagtga aacccagaac cttcgctact tcacgcgctg
     3121 tcgtggactt ccccgagctt gcaggaccat caatcgccac gaccatccac gcactccacc
     3181 tgtttccaca gccgttcaca ttcggctgcc gagagcactc gccacgccgc cgtggcaaga
     3241 ccggcgagac cgattccggc gaattcggtt cttttgagtc gaagaacctc atgtcccact
     3301 gcgcggcaca tgaggcgcac ctcccgtttt ctgccctctc gaagaaccaa ctccagctca
     3361 gaggtatcct tgcgcttctc gcacaccctc acttcggcgg ggctcgtcgt accaccttct
     3421 atttccacgc cgttgcgaag gcgatctaat gcttgagcat cgggatgccc cttcaccagc
     3481 gcgcgataca cgcgtggtat gcgataacgc ggatgcgtaa ggtgctcgac cagtccccca
     3541 tcgttggtca tcagcaacac accttcggtg tcgaggtcca accttccgac ggggaccaga
     3601 tgcgcgtact cgctcggcaa caggtcgtac accgttctgg tgtagtgagg atccctccgc
     3661 gagacaaggt agccggaagg tttgttcatc acgagagtcg tttttgactc gacgggagtg
     3721 atcacgcgac cgtcaagaca aacgacatcc tgggtcgtat cgatccgagc gcccagttcc
     3781 gcgggcagac cattcacggt aacgcgcgaa gaagcgatga gcttgtcggc acttcttcgc
     3841 gaagcgacac cgcaccgcga aagaaacacg ttcacccgaa caacgcaagc attgtcggct
     3901 ggcgcattca caggaacctc ttgttttcac agaatctttt aattttacgc tcaatatggg
     3961 gtcggcgtca agtaacgggc cggcggtgac tctaggtgac acacccggta cgggtgttcg
     4021 ttgagcggaa tgttccgctg atcacactgt acttctcggc cttccacggc gtgcgaaacg
     4081 cgtagactct atgatcatac gatccgacca caatctccag actcccatct ccgttgacat
     4141 cagcaatggc gggagtgcca aaaatctccg ctcctgtgag ataatcatcc acctcccggc
     4201 cttcttcgtc gatgacgtaa acacggttgt cgtctgatcc tacgacgatc cgcgcctctc
     4261 ctgcgatact ggcggggacg accactggag aagagcgaac cgcacctccc gtctggaact
     4321 gccatgacag ccggccggca caatcgatgc cgtgtacacg tccgtcatcc gagccgatca
     4381 ccacgcaggc tttttttccg tcacctggca gcacagcggg tgaagaaaac actcgcccgg
     4441 cggtagtggc ttcccacaga agcgttccgt cgaggtcgaa cgcgagcacc ttccccgtaa
     4501 caaccccgag aacgagagcc ggctttcccg ctccgaggag ttgacaaaga agaggggacg
     4561 agtagatcgc attcggacct ccttccgcga gcgcgtattt ctttcccgaa ggcacacctt
     4621 ggaacgaagt ccgatgaatc tctcccccga caagagcctg ccgccaccgg attcccccgt
     4681 ccgctttcag acacgcaatc gtaccctgca gggtcaccac gatgatttcc cgcgcgggag
     4741 taatcaccgg cgccgcaaaa atcggctcca ggagatcgac attccacagc cgttcgccgc
     4801 gcagattgag gcaggacacc gtcccatcca gcgacgctgc cacgatctcc tcaattccgt
     4861 cgccattcag gtcgccggca gcgggtgcgg aatccacacg attacctgtc gcgaattccc
     4921 atacgtgccg cccctgcccg tcgaggcaat agagcctgcc gtcggcagcg cccacaacta
     4981 ccctggcacc gccgccgtct cggccatcag taatcaaggg tgaggagaag tgcggatatt
     5041 cgtatcgttc acccctcgtc gtggttgtcc acattctcct cccgttgccg tcgagcgcca
     5101 cgatttcttt gtgggaagac accgcaacca ccgagaggga cccattgccc tccaagtccc
     5161 cgattgcggg tgacgcagca acgtacaacc cgagaggtgt cgaccaggca gttgacttgc
     5221 tgtggatgct ttccgctacc ctccacacat tgcgaagcgg ttccggacgc actcgcgaaa
     5281 aacccttccc atggacatgc tcaatgtctt cggcaacccg ttcgagattc ctccccgctc
     5341 tttcaaacca ttctctcagc cggggggtgg ggtcacgttc caggattttg tggaggtaga
     5401 caacctccgt catcgcccgc cacgctcgaa cctgaacccg cattttctcg aaaccggtgt
     5461 ggagtatctc gtacttcgcg gtttcgagtt tgcttctgat ggattcgata tccgcaatac
     5521 tcctttcaca caggctcagc gcccggtcct tttcccacag aatctgcacc aacgtttcct
     5581 cagttggatt cgccaattgg tctgccagaa cggcgagctt cgtcttccat tgcccgtgga
     5641 aatcgcggta gaaacgaatc atggaatcag cttctgcctc caccagggac ggagagctcg
     5701 tacacacgtt cagtatgcct ttcgtgtaga acgtgagctg aacaatctga ttcgtcctct
     5761 ggagcgcccg cccgatggca tccacggcgt cggatgggta tttcgatgtc agccaatcgc
     5821 gccagagact ttccaggtcc ttgtccaggt tctttgccag ttcggagaac gcaatcgtat
     5881 tgaactcatt cagggacccg aacgtactgc gctctgcacg gtccacccgc gcaatagctc
     5941 ccgtgaggcc aagtgatgcg gcgtatctca gacgattgcg tgtgtgctcg taactcaccc
     6001 acgggaaatc tgtccccccg gcgtattccc cccagcaatc gaactctatc atctgtggat
     6061 ggcgaccaaa aagtcccagg agtggattcg gtggctgcgc ctgataccag tctccccacg
     6121 actctttcgt catgatcgtc acgtgatccg gaacgtcgcg catcgcttcg gcataccaga
     6181 gcatcgtcct cggcacccag gtgaacgtcc tgacgatgag ccgcgccttg tgttttcggc
     6241 atatcgcatc gagcatgctt accatccgcg cgatcctgtg cggttgcggt cggtcgctta
     6301 ccacctcatt gtcgtcatct atgcggattc gggtctccgt gagtgtgagg accaccccat
     6361 caagttcagg aaccgcggag aacaaatcct cgtatttctg ggcaacgtaa tcccacgttc
     6421 ggcgcgaatt caggtcaacc ttgccaccca gcttgagatg gtcgggcacg ttctgcattt
     6481 cgtgcgtcca gacgaacacc tcgataccgt gccggtgagc ccgctcgatg agttctgcgg
     6541 catcacgctg aagctccgag gattcgacga tctgctcggc gtatgcgcag aaagagtgcg
     6601 acagttgtac gtggttgatt ccgaaccgag gcgcaagctc gatctcctgt ctcagcaggt
     6661 ccatgctgcg gcttgcaagg agccatcctc tcgtagagaa agacatcgca gagccctccc
     6721 cgcgcaggtg aacaacaacc cgtccggcgt gatcacacat gcttccagtg tgttctcacg
     6781 tctaagatac agaagcagaa gcgaaaatga aggggaattg cggaggtcac cagcgccacg
     6841 gcagtggtgc cgccagagat ctgaggaaag gcagttcgag agggacttct cgttcgttga
     6901 attcgatacc gaaacagggt gcggcaccgt aatggcagat ctgctgcatc agctcgggcc
     6961 agtcaatggt tcccgacgac ggcgcaacgt gttggtcatc aatgccgtta ttgtcattca
     7021 agtgaaggta atacacacga tttccgaatt gctcaaggta ggcgcgcgta ttgccggtca
     7081 cgtgcgcatg ccccgtatca agtgtcatac cgacgtgcgg cgactggatt gcgctgaaca
     7141 ggaacgcgaa atcgtacggt tgatcgccaa gagggttggg gtcgtggcac agcgtgttgt
     7201 cctcccaaca gagcatgact ccagtgtcct cgagcgcggg aaggattctt tccgtcagct
     7261 tcagtacccg atcgagtgcg cggtttctcc cgtctttttc cgcgagatcg aacaaaccaa
     7321 ggtggcatac gaggtggcgc ccgcccatga gatgtgtgcg cgccactgca ctttccagcc
     7381 gatcaaatgc gatgtcgacg ggggtctggc cgaagttgta gactccgtcc cacggagcgt
     7441 ggtaatcgac gaacaaacct gcatcctgtg ccatttggcg gatttcccgg cattcggcaa
     7501 ccgtgtactc ccgggtgaaa aactccacgc cgtcgagttc aagggcacaa caagtgtcga
     7561 tcgcctgact cacggattcg tgagcaatat gcgctgtact gagtccaaaa accggtttgt
     7621 tcacgtccat ttccaacgaa aaaccttccc gactcagttc agatgccaat atcctcgggg
     7681 atgtcgaatc cgtcctggaa atccgattcg tcgatgtgga tggattccag tcgattgggt
     7741 tcgagggccg ccctcagagc ggctgcaacg tccggatgaa actgggtacc cgcgtgtttc
     7801 tgaatttcgt ctgccgcctc aactctggtt cgtggcttgt tgtacgttcg tttgctcgtg
     7861 attgcgtcgt acgcgtcagc aacgaaaatt atggccgact ccaccggtat ctgatcgcct
     7921 ttgaggccgt cgggatatcc ttgcccgtcg taacgttcgt ggtgccagcg gactatcggc
     7981 gccacgtctg cgaactggtc tattttgcgc acgatccgtt ctccaatcag cggatgcgct
     8041 ttcagctgca ggtactcaag gtcatcgaga cgcgtagttt tgcgcaacga ctgatcgaga
     8101 acgcctattt tacccacatc gtggagcaat gcacccacgt ggacccgcca catcctttcc
     8161 tgttcaagat gcatcgccgc gcctatcata agactcagtt ccattacgcg acgggaatgc
     8221 ccgctggtgt agggatcctt ggcttcaatc gcctcaacaa tcgcatctac cgtctgcacc
     8281 tgtgagacaa agaggctggc tttcagttcc ccgatcgccc gctggtgatt cctttccata
     8341 tccgagagct gcttcgtaag ttcttcaatc tcgcgacgca acgctcgtat gtacggttcc
     8401 tgaggctctc gttggttcat cttagaggtc gtatccatcc ctacgtccgg aacgggagcc
     8461 ctcgccagca accttcgttg cacgtcactc agcgaacacc gctcacgtct ccggtacgaa
     8521 aacacagaag cgcgcgtttt cccgtttcat gcagttcttt cgaaattaac cccgttgcgc
     8581 gcccgctcca acgcctgaac gaacctcgtc acgcccgcca gcgctctttt tgcagggttc
     8641 tcgcagggct gccgatgatc ccacatcccg cgcgccgggg aactccccgg acgggtaatc
     8701 cgtactatta acgaagaaca tagacaggga gggaacggaa caatggaaac tattcgcgtc
     8761 cacagcgatt tccacgcggc tcacaggcaa atcgggtatg ccggcaaatg ccggcacgtg
     8821 cacggtcaca catggcgagg gacgatctcg ttgagcacgg agaagttccc aaggaaccac
     8881 cttgacatgg caatagattt cggagacctc aaacaggtgt tccgccggat ggaccacaag
     8941 atgctggtgg gagaagccga cagagaattt gtcacggctc aaggagtaga cccggaaggc
     9001 gttgtggtga ttccgggttc caatcccagc gtggaaaaca tcgcatatta ctgtctggat
     9061 ggagtcagga aggtgatcga gcaccagtac ccgggcaaag gactcaaata ccacctggaa
     9121 ctgacacttc aggaaacgga aaacaacatc ttcgtagtgg aaactgattt cgtgatctga
     9181 ggaacaggtc ttgggagaag ccgcccgggt tggctaattt cttcagtcgg tcggcgaggt
     9241 tgcgcgcgca caaactgtgc ctgcacattt gtggtggaaa agggaggtcg gcggagtggg
     9301 tttccgggct ttggtaggat cagtctggtg tgcgcttctc ggaatatcgt tcgccgcgtg
     9361 cggcaacaac gtcgccaaac tggtgagtcc gcttcagacg agcggaagtg tcacgatcga
     9421 gatctctcgc tctacaactc ccgttttttc gtggaacgca acaactacca tcgaagccat
     9481 cagtgtcgcg aatttccgcg ttgatggaac tgtggacagg atactgtggg gataccgcgg
     9541 gatcgccgcc actccaccaa tcacctacgg aagcgtaatc cccggaggaa taagtctctc
     9601 cgtcggaaca ccacctgcgc ttcagagtgg aaatcggtac cgcgtcgagg tggtcagcgg
     9661 cggcaaagcg agcactgcgg attggatcgt gccctgaggg ttatgcgcga atggcagcgc
     9721 cggattctgc gtcgaaaaag tgcattttgt cagggcgcgg gatcaggttg attgtagaac
     9781 cgttcggcgg ggccacatga gcgtccacac gcgcgatcag ggcatggttt gcagtccgtg
     9841 cgtacacaaa cgtttcactc cccatgggtt ccacgagttc aaccctggca tcaaatgagc
     9901 ccgatgccac cttgtcgttg ctgagaagca acgattctgg tcgtattccc agcaccacct
     9961 tcttccctga atactcgtga agcgcggatg tcaggtgtac aggcaccggt agtcggaagc
    10021 tcccttcatt gaacaccgtt ccagttccat cctcgataat cccctcaaca aaattcatgg
    10081 caggcgaacc tatgaatccg gccacgaact tgttcctagg agcgtcgtaa agattgatcg
    10141 gcgtatcgat ctgctgaata gtgcccgcat ccataacgac gatcctgtct cccatcgtca
    10201 tcgcttccac ttggtcgtgg gtgacgtaga tcatcgtcgc gcccagcctg tcgtgcaact
    10261 tggatatctc ggtgcgcatc gccacgcgaa atttcgcatc caggttcgac aacggttcat
    10321 cgaaaagaaa caccctgggc tttcggacaa tcgcccgtcc cactgcaacc ctctggcgct
    10381 gcccgccgga gagtgctttc ggtttcctct ccagaagctc cccaatttca agaatctggg
    10441 cagcttctct gacgcgcgca tctatttctg tccggggata cttccggagc ttcagaccaa
    10501 aggccatgtt ttcgtacacg gtcatatgtg ggtaaagcgc ataattctgg aacaccatcg
    10561 cgatgtcccg gtccttcggt ggtatgtcgt taaccaaccg accgtcgata tagatttccc
    10621 ccgagctgat ttcctccaac cccgcgatca tcctcaacgt cgtagttttc ccgcagccgg
    10681 aagggccgac aagaacgacg aactccttgt cagcaacctc aaggttcgcg tcccgaacgg
    10741 cgaaaactct ctgatcgaat acctttgtga cgttcttcag cactaccgtt gccatcttca
    10801 gactcgctct cctgtggcag ttgcaccggc agcccccgac gttcaaacgg gagcaagaac
    10861 cttcgtcaac ctcgcaacct cctccttgag actgcggcga tcaacaacgc ggtcgacgaa
    10921 tccatgctca agcaagaact ctgcgcgctg gaaacccggc ggcaggtcct ccttgatcgt
    10981 ttcccggaca cgcgctccgg cgaacccgat caatgctccc ggttcggcaa gaatgacgtc
    11041 tccaagcatg gcaaagctgg ccgtaacccc gcctgtggtc gggcttgtca gaacgctgat
    11101 gaagggaagc gatgattccg caaactcggc caatcgtgcg ctcgttttcg ccatctgcat
    11161 gagagacagc gccgcttcct gcatcctcgc acccccggat tggcttacga tcacaagggg
    11221 tactcttcgg tcctgtgcga aaccgacgag gcgattgatc ttttcgcctt ccgctgaacc
    11281 gagactcccg ccccgaaacg agaagtccat cgcgccaacc gcaaaaggcc tgccgtagat
    11341 agccccggac gccgccatga atgcgctgcc gcgacccgtt ttgcggaagt cctccatgat
    11401 ctggtcgccg taccgtttct ccgctttgaa ttgcagcggg tcggccgaca ttacggtttc
    11461 gaacaagggc tcggccgttc cttcgtccag gagcaaatca aggtatgcgt gcgccccgat
    11521 tcgcatgtga aagccacacg atgggcacac ccacaggttt tcttccaggt cgcgcctgta
    11581 caatgtctta ccgcaaccaa gttgagcgtc ggcgcctttg ccttcgcact tgacccacac
    11641 accgtctgga atcgctttct tcgcgttcct ggcaaagcca gctttccacg cgtcaagcca
    11701 acccatagca tctccttcca gaagcgcctc gcacgtactc gtcctcaagc catctttcaa
    11761 tcaggcgcgc agctttcccc agagacattt caagcacctc gagcacctca tcgtgccgaa
    11821 ttcgcgccag cggactggaa ggaacgagat tcgtcacaca cgaaactgct gcggcgtcga
    11881 gacccgcaag gcgtgcccac cgcgattccg gaagcgttga catgcttacc acggacgccc
    11941 ctgcagctcg tatcatgcct acttccgccg gcgtttcgta gcacggcccg gtcatcatgg
    12001 caagcacacc cttcgaaaat cgaacgttca ccgcgcttcc agctcggtcg aacgcgttct
    12061 ccaaacgttc agaataacgt gggatgtgag agaaacgctt ccccgcgtgt tgcctgaacc
    12121 ctttcgcgtt ccagagaaaa atatcgtcct ccgcgaggac gagatcgcca acgcgaagat
    12181 gacttgccac accaccggcg gcgttcgcgc tcaacaacca gcggcacccg agcgcgcacg
    12241 caagcctgcc g
//
LOCUS       DAOSOO010000082        12245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5170_length_12245_cov_5.028958, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000082 DAOSOO010000000
VERSION     DAOSOO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_12245_cov_5.028958"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLT86_07475"
     CDS             complement(<1..253)
                     /locus_tag="PLT86_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36488.1"
                     /translation="MGEIWTLLFVDDKHIEATRRVCRVFEQPRKFDPVVVPDRPWEAH
                     CVIVYGSVLPDPAGSGFQMWYQSYDRSKNGPDRAHNCYAV"
     gene            complement(326..>1775)
                     /locus_tag="PLT86_07480"
     CDS             complement(326..>1775)
                     /locus_tag="PLT86_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOT36489.1"
                     /translation="IEHLPFPMAPVAAGGATALAETSTKSETWRWRIFSIGTVIGLIW
                     GTIYVVIPTLSGTFLTQAVAILPIPFLDFTVNLRSLLPTAVLAISTDMSTLLAGFVFP
                     YWTVIGMFIGSMVKNFVVNPMLYNYGILHRWEPGMSLIPTTIAVEFDYWLSAKIGMAV
                     AVFVISFYAVIRKLITGKDEISERPTDNLDPEKVGPGSGRGDLPLWIPITAWFVCTTS
                     IVFFVHYLVPDFPWWITAFFGFIFTPILSYISAKMTGITGSAAGTSFPYVREGAIYLS
                     GYQGVAIWFAPLPIGDYGGFSSTFRVLQLTRTKFISYIKLIVANLLILTTFSFIFWTL
                     IWRMTLIPSSEFPYVQKMWPYFATMQALWASSTLPGGNSLMQGIVTMPKLVSGSITAF
                     GLYGIVLLFKAPLSLFYGMIGGLTGFPTGTYLLFAGAMLNRFYFYKKFGQATWAKYAP
                     ILLAGYGCGMGLVAMSSIAVSLIVKSVSQVIF"
     assembly_gap    1776..1785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1786..2331)
                     /locus_tag="PLT86_07485"
     CDS             complement(<1786..2331)
                     /locus_tag="PLT86_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36490.1"
                     /translation="MATDSTEVRTKPARPEDLADVEEPFTDGFNWNTIMAMLFIGIVM
                     MPGAIYLGLVAGQGMGGAAEWVTIILFLEITKRSFITLKRQELILIYWSASMLVSVAG
                     SGLSGGIFAGLIWNQFFVQSPQAEAIAAYIPTWYAPPKGSAALLERTFMDAVWIQPVA
                     VVVISTILFQIVSLSLGYVIFR"
     gene            complement(2356..>3999)
                     /locus_tag="PLT86_07490"
     CDS             complement(2356..>3999)
                     /locus_tag="PLT86_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT36491.1"
                     /translation="IKRDNPPAYDGMLVRDRNWFGLQASVLDYVQTAFRGKALITPRS
                     WYMAQMKADKAFIDFRNPANDKRSFASGILGVEADEAKVTALDKALVAGRWFAPGERG
                     VCILPSRMAELAGVTAADVGTAKLEMLGRVWTVIGLVDEQGISRRPDLDAEILTPVDL
                     VAEQQRQTEASAEDPTQMAEAPIQTFTHIAPENVLILPHVDVMEMGGMLRSVAVSNFA
                     EPHEMLSQIEGFMSRVALTTFVGHDNKVEVYSSLGATSLKGLSNLGVPIVVAALIVLN
                     TMLGAVYDRKREIGIFATIGLTPFHIAALFIAEACVFAVIGAVWGYLIGQVLAIALSQ
                     TSLLAGMSLNYSSLSAVASTLIVMATVVLSSLYPAKVAADSAVPDVTRRWSFPPADGD
                     YWRFDFPFTVGESDVIGLYAYLQRVLEAYGAGSAGEFVAQNVELEALEVKEGEDPQYR
                     LSFRTWLAPYDLGISQDVQMDAVPTGEYRIYRIEMTIHRLSGDVDSWQRINRGFMNVM
                     RKRFLVWRTIPPALKDEYRERGKRIFRGETVGSTVNVDG"
     assembly_gap    4000..4099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4100..7431)
                     /locus_tag="PLT86_07495"
     CDS             complement(<4100..7431)
                     /locus_tag="PLT86_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36492.1"
                     /translation="MAEVPQTSGDVPANGNGVNSSLWRLVGKIGKWLLIVFAVAQLLA
                     VTVFDPEKVSVWLFGLDLNPQRATRVDFGRVDAESQAVMDAVTRDNVARHMAAIVTGE
                     SRAVGYPGERKTFEYVRSEFQRLGLQDMGVDSFEVVVPRDEGGSVTFGGGETKPIYAL
                     WPNQVKTPTLPRAQISLNDTLLGPVSAVFPSKPSTFELPPEGVAAEFVTIPRHDLSLT
                     PEEQERFGARRGQVGIWDVVDFSRHQVRGKFVVFDLIGSAAKAKGSSETINIDSANVM
                     GARAAGARGVVFRDYQVTVLLNPERRLPELPDDILYFSADNQIAARLKDKNGNSSGVL
                     RFTPGIGGELLYAGSGDFHSFNGRKVEGSIVVMDFSTQKNYLNAHELGAQAIIFVDDG
                     KITREEAEAKFHLVPVDVARFWAEGACADTLRQLGSQPGNRVVLRSRMQWSPVRTYNI
                     WGRIPGTRTNRPEDTIVLEAYYDANSVVPARAMGAENAAGIAGLLAIAESFSQHPPEL
                     PILFLATSGHHSALSGINEWLYRHSRTENAFLQRIPQNQRIPFGLFIGLDLSSQNDQV
                     GSFAQGTFYTGWNTDARRTNMHTGYGKAFMRYAEELFPGQQVNEAYPQFVDGIAPAKR
                     TWRTFLPVAMAFDHEPVNYVGLSAITLATTNDIRERVDTPVDSLGAVNIDNVATQART
                     IAGLIHRGARDEGFFQETKIKPKDTQRELTGRILQFDRRKSFIPSTQIGGAIVTTSYG
                     NVKSFCGVRGMLATLTNSGPDDGLDCTRWTLTDTVATNQIPSQVGRFRFHMVGPHVPE
                     VMIKAYEIGDDGRIVMAPDLGQEGDKTYPMRVRADSPLLQTLQVLFEARALSLFEIID
                     SRYLSALDMMSVRGPDGSELRSYGMEVVPLQSRSEGKVESGAVAFAQPGTRVKVLMST
                     SLFGIKYLLINAPDEFFTNPLKPSEVTPAHQIRAEGSGYPVDLGRIDRTSWRVARDIW
                     VIDDVRMKTLTRFGIVNQRVMKLHEDAREALLQAKKAYDKQEYDGFIAMSRRAWGYEA
                     RAYPDVKGTAIDTIQGVIFYFALLLPFAFFMERLVIGAVDIRRQILGFGLIFVGIFFV
                     LQFVHPAFKLSTSPYIIFLGFV"
     gene            complement(7735..8682)
                     /locus_tag="PLT86_07500"
     CDS             complement(7735..8682)
                     /locus_tag="PLT86_07500"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT36493.1"
                     /translation="MIRKDELEKRYPLPRGIISVLQTPFDDNRDVDTKSLERLVEHAI
                     GAGVNGFLVPAVASEVDTLSTKEREHVVEVVLKASGRRVPVIVGASAPSEEDCATFAR
                     YAERVHADAYLAAVPSFLYAHQEKVQPFFAHVAASTSLPLVIQDLQFGGYGLRIEEIE
                     RLWETIPRLAGFKVETVPAGPKYTAIREKLGASVFVAGGWAIPQMIEALDRGVDALVP
                     ESSMVPVYRRVYELHESGRRSEAVTLFRRILPVLSYTNQELLTSIAFFKRLLCRKGIF
                     AREHMRLPGFQWDRFSERIAEELIALVLEVEATVSVRTG"
     gene            8708..9196
                     /locus_tag="PLT86_07505"
     CDS             8708..9196
                     /locus_tag="PLT86_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HOT36494.1"
                     /translation="MNFKGNVVFWLGTLGAFAWGIEQVLAYTWFLSNVLYFSIPEGAF
                     RVVLALMVLCTCAAGFAALRNPEGIRIRLWLACNGFWLAVALCTILFAIRFRGYVPGL
                     FTSCIVVIPGSAFALWRAYAARFLPPDNVRTWTIAGAAAFLPLVVVLTGAGWLAFELC
                     TP"
     gene            complement(9208..10287)
                     /locus_tag="PLT86_07510"
     CDS             complement(9208..10287)
                     /locus_tag="PLT86_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36495.1"
                     /translation="MNVNIEPYLMRLNDVFDPERSSKSVERQKNVCLFHKSDRLPVAF
                     VLDEVPGWERIRLDEAYACPELMLLNELRPIYGSALVGDDRANTIRANFGVGILPSLF
                     GCEIKLTYDAMPWVEPLNNREAVAKIVSAGALDLRGGLWARIEEFQEFAIAQLADFPA
                     LRSGIRLSMCDMQGPFSIAHLVWGNDIYYAVVDDPDLVRDFLELVTDVYIRFVRQERE
                     ATKADEEFTEFLNVLVPGKILIREDSATNLSPAMYEEFCLPYIQKTLDAFPGGVHYCG
                     AGHQFFELVAGCRNLTAMNFGNPEMQRPERVRSLAARGIAVVGWGLWPLDHPLDELET
                     GISLGISVHDVDEGKRIMDQVASRG"
     gene            complement(10297..>12245)
                     /locus_tag="PLT86_07515"
     CDS             complement(10297..>12245)
                     /locus_tag="PLT86_07515"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT36496.1"
                     /translation="LSDPYKAIVALRLFPEDSLSQDGLEHVVTDERENVAAIARFFTG
                     SSENAGQIRRTGTVGEVSITRNHEILVTIGKEGVETGRLAEAVLKDPRRIREFDRGAT
                     IVIPADAMKDGYRAAVLTHLFFSDVIREDSRRHIVTLPIESLSRVSLWFTGTGENWPL
                     LKRASGKRSNDVAKGEVIVIPRNLLGSWALMTEEDISEETRDYPLALTSRSRRDAVNL
                     GQRLLIPKRLLSAWTALMEKGEDGAQDAAKWYVYWGTRNPPLVYSGDRLGGYAGYRLQ
                     RGQALYSDVVIRFTGVVEVAEVLHLSEVVLRRSGFRDPRKLPVNALIKIPIGLLSPEW
                     RPEGDPARLAEREEDTAVARVEEEIRVEKAEARAAEPVRPRGEALRGVTIVLDAGHGG
                     HDPGAIGPRGLTENEVVYDIMCRVMRQFAQRTQATVYVTIEDARTGHTPSERATIPDN
                     REEHLLTTPKYPNADVVTSANLRWYLANSYVRRAINTGTDLERVVFTSFHADALHPSM
                     QGCMVYVPSARHSKGRYSAGTRYAIYHEVSERPTVSYSYNERLRSQALSSGFADALCQ
                     RLRSANVGVHGRKPVRGYIIRRSGGGEWVPAILKYNAAPTKILVEIGNIRNENDAANM
                     RDPAWRERFASAYVDAVISHFSTQ"
BASE COUNT         2593 a         3707 c         3315 g         2520 t
ORIGIN      
        1 tgaccgcgta gcagttgtgt gcccggtcgg gaccattttt ggaacggtca tagctctggt
       61 accacatctg gaaaccggac cccgccgggt caggaagcac ggaaccgtac acgatcacgc
      121 aatgggcttc ccacggccgg tctggaacca caaccgggtc gaatttccgc ggctgttcaa
      181 acacacggca aacgcgtctt gtcgcttcaa tatgcttatc gtcgacgaag agcagagtcc
      241 agatttctcc catctgaagc cgcttcaatg aaatgggcgc ccgcgaaagg cgcccgtttg
      301 ttcagcttgc cccggcacac gtgcctcaga agataacctg agacacgctt ttcacaatca
      361 gcgacactgc aatggaggac atggcgacca gacccatgcc gcatccgtag cccgcaagca
      421 ggatcggcgc gtattttgcc cacgttgcct gcccgaactt cttgtagaag tagaaccggt
      481 tcaacattgc ccctgcaaag agcaaatagg tgccggtggg gaaaccggtc aggccaccga
      541 tcataccgta gaacagactc agcggcgcct tgaacagcag aacgattcca tacagaccga
      601 acgccgtgat cgaccccgat accagtttcg gcatcgtcac gatcccctgc atcaggctgt
      661 tcccccccgg caacgtgctg gatgcccaca gcgcctgcat cgtcgcgaag tacggccaca
      721 ttttctgcac gtacgggaat tccgaggacg gaatcagagt catccgccag atcagtgtcc
      781 agaagatgaa actgaaggtc gtgagaatca gcagattcgc cacgatcagc ttgatgtaac
      841 tgatgaactt cgtgcgcgtc agctgaagca cccggaaggt ggatgagaac ccgccgtagt
      901 cgccaatcgg caacggcgcg aaccatatcg caaccccctg gtaccccgaa agataaatcg
      961 caccctcgcg cacgtaaggg aaggatgtcc cggcggcgct tccggtgatt cccgtcatct
     1021 tcgcggagat ataactcaga atcggcgtga agatgaaacc gaagaatgcg gtgatccacc
     1081 aagggaaatc gggcacaagg taatgcacga agaaaacgat actcgtggtg catacgaacc
     1141 atgcggtgat cgggatccac agcgggaggt cgccacgtcc acttcccggg cccactttct
     1201 ccgggtcgag gttgtccgtc gggcgttcgc tgatctcgtc cttccccgta atcagcttgc
     1261 gaatcaccgc atagaaactg atgacaaaca ctgccaccgc catcccgatt tttgcggaga
     1321 gccagtaatc aaactccacc gcgatggtcg tcggaatcag cgacattccc ggctcccatc
     1381 gatgaaggat tccgtagttg tacagcatcg ggttcacaac gaagttcttc accatggaac
     1441 cgatgaacat cccgatcacg gtccagtacg ggaatacgaa tccggcgaga agcgtgctca
     1501 tgtccgtgct gatggcaagc acggcggtcg ggagaaggga acgcaggttc accgtgaaat
     1561 caaggaaggg gatgggaaga atcgctactg cttgagtcag gaaggttccc gaaagcgttg
     1621 gtatgacgac gtagatagtg ccccagataa ggccgataac ggtgccgatg gagaagatcc
     1681 gccaacgcca cgtttcactt ttcgtggatg tctccgccag ggcagttgcg ccacccgcag
     1741 ccaccggagc catcgggaag ggcaagtgct cgatgnnnnn nnnnnccgga atatcacgta
     1801 cccgagcgaa agcgacacaa tttgaaacag gatcgtactt atgacgacga ccgcgacagg
     1861 ctgaatccac accgcatcca tgaacgtgcg ctcaagcagc gccgcactgc ctttcggtgg
     1921 tgcataccat gtcggtatat acgccgcgat cgcttcagcc tgcggggatt gaacaaagaa
     1981 ctggttccat atcagcccgg caaaaatacc gccggaaagg cctgatccgg caacgctgac
     2041 cagcatcgac gccgaccagt agatgaggat cagttcctgc cgtttcaggg taataaacga
     2101 tctcttcgta atctccagga aaaggatgat ggtaacccat tctgccgcac cacccatgcc
     2161 ctggcccgca accagaccca gatatatggc accgggcatc atgacgatac cgatgaacag
     2221 catcgccatg atcgtgttcc agttgaaccc gtcggtaaag ggttcttcga catccgccag
     2281 gtcttctggc cgcgcgggtt ttgttcgtac ttccgtagaa tccgtcgcca cgtctactct
     2341 cctgacggga gcgtgttagc catcgacatt gaccgtactt cccacagtct cgcccctgaa
     2401 tatccgtttg cctctctcgc ggtactcgtc tttgagagcc ggggggatag ttctccacac
     2461 gaggaagcgc ttccgcatga cgttcatgaa accacggttg atccgttgcc acgaatcaac
     2521 gtcgccactc agtctgtgga tcgtcatttc gatccggtaa attcgatact cgccggtcgg
     2581 caccgcgtcc atctgaacgt cctgactgat gccgaggtcg tacggcgcca accaggtccg
     2641 gaacgacaac cgatactgcg ggtcctcgcc ttccttcacc tcaagagcct caagttccac
     2701 gttctgcgca acgaactccc ctgctgatcc ggcgccgtat gcctccagaa cccgctgcag
     2761 gtacgcgtaa aggccgatga cgtcgctttc gcccacggtg aacgggaaat cgaagcgcca
     2821 gtaatcgccg tccgccggcg ggaatgacca tcggcgggtg acatccggca ctgcgctatc
     2881 ggcggcaact ttggcgggat acagagagga aagtacaacg gtagccatca ctatcagcgt
     2941 ggaagcaacc gccgaaaggg aggagtaatt cagcgacatg cccgcgagaa ggctggtctg
     3001 gctcagcgca atggcgagca cctgtccgat aaggtacccc cacacggccc caatcaccgc
     3061 aaatacgcac gcttccgcga taaacagagc ggcaatgtgg aacggcgtaa gacctatcgt
     3121 cgcaaaaatc ccgatctcgc gctttctgtc gtacaccgcg cccagcattg tgttaaggac
     3181 aatcagcgcc gccactacga tcggtacacc caggttcgac aaacctttga gcgatgtcgc
     3241 accaagactg ctgtacactt ccaccttgtt gtcgtgcccg acaaatgtgg taagtgccac
     3301 gcgggacatg aagccctcaa tctgcgaaag catttcgtgc ggttccgcaa aattgctcac
     3361 cgccacggac cgcagcatgc cacccatttc catgacatcc acatgcggca gaatcaggac
     3421 attctctgga gcaatgtgcg tgaacgtctg tatgggtgct tccgccatct gcgtcggatc
     3481 ttccgcggac gcttcggttt gcctttgctg ttctgccaca aggtcaaccg gtgtcaaaat
     3541 ttccgcgtca aggtccgggc gtctggagat cccctgctcg tccacaagcc cgataaccgt
     3601 ccacaccctt ccgagcatct cgagtttcgc ggtgccgaca tcggcggcgg ttactccggc
     3661 cagctccgcc atccgagagg gaagaatgca cacaccacgt tcgcccggcg caaaccagcg
     3721 tcccgccaca agtgctttgt ccagcgcggt aaccttcgct tcatctgcct caacaccgag
     3781 gattcccgag gcaaaactcc gtttgtcgtt tgcgggattg cggaaatcaa taaatgcctt
     3841 gtcggccttc atctgcgcca tgtaccaact ccgcggcgta atgagcgctt tgccgcgaaa
     3901 cgcagtctgg acgtaatcga gaacgctcgc ctgcagtccg aaccagttgc ggtccctcac
     3961 gagcatcccg tcatacgcag ggggattatc ccgcttgatn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnna tcacgaagcc caggaaaatg atgtaaggtg aagtactgag
     4141 cttgaacgcg gggtggacaa actgcagcac gaaaaagatg ccgacaaaaa tgagtccgaa
     4201 cccgagtatc tgcctgcgta tgtctaccgc accgatgaca agtcgttcca tgaaaaatgc
     4261 aaacggcagc agtaaggcga aatagaaaat cacaccttga atcgtatcta tcgcggttcc
     4321 tttgacgtcc gggtacgccc tcgcttcata cccccacgcg cggcgagaca tcgcgatgaa
     4381 accgtcgtat tcctgcttgt cgtacgcctt cttcgcctgc aagagagctt cccgggcgtc
     4441 ttcatgcagc ttcatgacgc gctgattgac gatgccgaat cgcgtcaagg tcttcatccg
     4501 cacgtcgtca ataacccaga tgtcgcgcgc gactcgccac gacgtccgat ctattctgcc
     4561 gaggtcaacg gggtaccccg acccttccgc gcggatctgg tgcgcaggtg tcacctccga
     4621 aggcttcaac ggattcgtga agaactcatc cggagcgttg atcagcaggt attttatgcc
     4681 gaaaaggctc gtgctcatca ggactttcac gcgcgtcccc ggctgcgcga acgcgacggc
     4741 gccgctttcc accttgcctt cggagcggct ctgcaacggg acaacttcca tcccgtagct
     4801 gcgcagttcc gacccgtctg gcccccgaac gctcatcatg tcgagcgcac tcaggtacct
     4861 gctgtcgatt atctcgaaaa gcgaaagggc acgtgcttcg aacagaacct gcagggtctg
     4921 caggagcggg ctgtctgcgc ggacgcgcat ggggtaggtc ttgtcgcctt cctggccgag
     4981 atccggtgcc atgacgatcc ggccatcatc gccgatttca tacgccttga tcattacttc
     5041 aggaacatga ggccccacca tgtggaaccg gaaacggccc acctggctcg gaatctggtt
     5101 cgtggcgact gtatcggtca gcgtccagcg cgtgcaatcc agcccgtcgt cagggccgga
     5161 attcgtcagg gtggccagca tcccgcgaac accgcagaat gacttcacgt tcccgtaact
     5221 cgtcgtgact atcgctccac caatttgcgt gcttggaatg aaggacttcc gccgatcgaa
     5281 ctggagaatc ctgcccgtga gttcacgctg ggtgtctttc ggcttgatct tcgtctcctg
     5341 gaagaaccct tcgtcgcgcg cgccgcgatg gatcagcccg gcgatagtgc gggcctgagt
     5401 cgccacattg tcaatattta ccgcgcccag ggaatcaacc ggcgtatcta ctcgttcccg
     5461 tatgtcattg gtcgtcgcca acgtaatcgc gctcaaaccc acgtaattga cgggctcgtg
     5521 gtcgaacgcc atcgccaccg gcaggaacgt gcgccacgtt cgttttgccg gtgcgattcc
     5581 gtccacgaac tgcgggtacg cctcgtttac ctgttgcccc gggaacagct cttcggcata
     5641 ccgcatgaac gctttgccgt acccggtgtg catattggtc cggcgcgcat cggtgttcca
     5701 tcccgtatag aacgtcccct gcgcaaagga gccgacctga tcgttctggc tggaaaggtc
     5761 cagcccgata aacaggccga acggtatgcg ctggttttgc ggtatgcgct ggaggaatgc
     5821 gttctcagta cgcgagtggc ggtacagcca ttcattgatt ccgctgagag cggagtgatg
     5881 cccggaggtg gcgaggaaaa gaatcggaag ctcaggagga tgctggctga aagactccgc
     5941 aatggccagc agaccggcga tccctgctgc attttcggcg cccatcgcgc gagccggaac
     6001 aaccgagttg gcgtcgtaat acgcctccag aacaatcgtg tcttccggtc tgttggtgcg
     6061 cgtcccggga atccgacccc agatgttgta ggtgcgaacg ggcgaccact gcatccggct
     6121 gcgcaagacc acccgattgc cgggctgact tccgagctgg cgcagagtgt cagcacatgc
     6181 gccttccgcc cagaatctgg caacatccac gggaacaagg tggaacttgg cttccgcttc
     6241 ttccctggtg atctttccgt cgtcaacgaa aatgatggcc tgcgcaccca gttcgtgtgc
     6301 gttcagatag ttcttctgcg tggaaaagtc catcacgacg atggaacctt caacttttct
     6361 tccgttgaag gagtgaaagt cgccacttcc ggcgtagaga agctccccgc ctattcccgg
     6421 cgtgaatctc agaacaccgg aggaattgcc attcttgtct ttcaagcgcg cggcaatctg
     6481 gttgtcagcg gagaaataga gaatgtcgtc aggcagttcg ggaagccgcc gttccgggtt
     6541 gagaagcacc gtaacctgat agtcccgaaa aaccacgccg cgagcgcctg ctgcgcgagc
     6601 ccccatcacg ttggcggagt caatattgat cgtttcggac gagcctttcg ctttcgccgc
     6661 gctcccgata agatcaaata cgacgaattt gcctcgaacc tggtggcgcg agaagtcaac
     6721 cacgtcccag attcccacct gcccgcggcg tgccccgaat cgttcctgtt cctctggcgt
     6781 aagcgacaga tcatgtcggg ggatcgtgac aaactcagcg gcaacgcctt ccgggggaag
     6841 ctcgaatgtg gagggtttgg aggggaacac tgcggagacc ggccccagaa gggtatcgtt
     6901 cagagatatc tgcgcccgtg gcaaggtggg cgttttcacc tggttcggcc acagggcgta
     6961 gatgggtttg gtttcgccgc cgccaaacgt gacgctgccg ccttcatcgc gcggcactac
     7021 cacttcaaac gagtctactc ccatgtcttg gagtccgagg cgctggaact cgcttcgcac
     7081 gtactcaaat gttttccgtt cgcccgggta ccccaccgcg cgcgattccc cggtcacgat
     7141 cgccgccatg tggcgcgcca cgttgtccct cgtgacggca tccatcacag cctggctctc
     7201 cgcatctacg cgcccgaaat ccacgcgggt tgcgcgctgg gggttcaagt ccagcccgaa
     7261 caaccagacg gatacttttt cgggatcaaa caccgtgacg gcaagcagtt gcgccacggc
     7321 aaacacgatg aggagccatt tgccgatctt gccaaccaat cgccacaaag aggaattcac
     7381 gccattcccg ttggcgggga cgtcgccaga tgtttgggga acctcagcca ttggcatctc
     7441 cgtacggtat tctcaaaacg tcgaaagctc ttgctccagg tcttgcggca atcttccggg
     7501 cgtagactcg ggcctggaat ccgttgtgta cggtgcagca ggcaatacct gtcgattcag
     7561 tagtggggcc tgaagtcagg cctgtggccc tctgctctct caagaaaacg gtgggaagct
     7621 cacatgagat cgcgaaaaac gccggttgaa catattccat cgaaaactct ggcacgcgtt
     7681 ccaatggaag tatacacacg aacgcggagg cggccaaccc gtgcttttcc ggaatcagcc
     7741 ggtgcgcaca gagaccgtgg cctccacttc cagaacaagg gcaatcagtt cctccgcaat
     7801 ccgctccgag aacctgtccc attggaaacc gggaagacgc atatgttcac gcgcgaaaat
     7861 gcctttgcga cagagaagtc gtttgaagaa ggcgatggag gtcagaagtt cctgatttgt
     7921 gtacgagagc accgggagaa tccgtcgaaa caacgttact gcttcgcttc tgcgtccgct
     7981 ttcatgaagc tcgtacaccc tccggtacac gggcaccatc gaactctccg gcaccagagc
     8041 gtctacgccg cggtcgagcg cttcgatcat ctgcggaatg gcccatcctc ccgcaacaaa
     8101 gacgctcgca cccagtttct cccgtatcgc ggtgtacttc ggacccgctg gaacggtttc
     8161 caccttgaac ccggcaagcc gaggaatggt ctcccacagg cgttctatct cctcgatccg
     8221 cagaccgtac ccaccgaact ggaggtcctg aatcaccaag gggagggaag tggaggcggc
     8281 aacatgggcg aaaaagggtt gaaccttctc ctgatgggcg taaaggaaag acggaaccgc
     8341 cgcaaggtac gcgtccgcgt ggacccgctc cgcgtaccgc gcaaacgtgg cgcaatcctc
     8401 ctccgacggt gccgatgctc cgacgattac cggtaccctc cgccctgaag ctttcagaac
     8461 aacctctacg acatgttccc gttccttcgt ggaaagggtg tccacttcgc tcgcaaccgc
     8521 gggtaccaga aacccgttca cacccgcccc gatagcgtgt tccaccaggc gctccagcga
     8581 tttcgtgtct acgtcccggt tgtcatcgaa gggagtctga agtacgctga ttattccacg
     8641 aggcaacggg taacgttttt ccaattcatc tttcctgatc atgtcttcat ccggcgcggg
     8701 aggtcacgtg aacttcaaag gcaatgtcgt tttctggtta ggcaccctcg gggcattcgc
     8761 gtggggaatc gaacaagtgc tggcgtacac ctggttcctc tcaaatgtcc tctacttctc
     8821 gattccggaa ggtgcctttc gagttgtact ggctttaatg gtgctgtgca cgtgcgcggc
     8881 cggattcgcg gcgttgcgca atcccgaagg tatacgaatc cgtttgtggc tggcgtgcaa
     8941 cggattctgg ttggctgtcg ccctttgcac gatcctcttc gccatacgct tccgcggcta
     9001 cgttccaggt ttgttcacct cgtgtatcgt cgtgatccct ggttcggcgt tcgccctttg
     9061 gcgtgcgtac gccgcacgat tcctgccacc cgacaacgtc agaacgtgga caatcgcggg
     9121 cgccgcagcc ttcctccctc tcgtggttgt tctgacaggc gcgggatggt tggcgttcga
     9181 gctgtgcacg ccgtaggaac ggaaaaatca tccgcgtgac gccacctgat ccatgatccg
     9241 cttgccctca tccacatcgt gaacggagat gcccaggctg ataccggttt ccagttcgtc
     9301 caacgggtga tccagcggcc aaagccccca tcccaccact gcaatgcccc gcgccgcgag
     9361 agacctgacc cgttcaggac gttgcatttc cgggtttccg aagttcatcg cggtcagatt
     9421 ccggcatccg gctaccagtt cgaagaattg gtgtcccgcg ccacagtaat gaacgcctcc
     9481 cggaaacgcg tcaagcgtct tctggatgta cggaagacaa aactcctcgt acatcgcggg
     9541 agagaggttt gttgccgagt cttctcgaat gagaatcttg ccgggaacca gtacattgag
     9601 aaactccgtg aactcttcgt cggcttttgt cgcttctcgt tcctgacgga caaaacggat
     9661 gtacacgtcg gtgaccaatt caaggaaatc ccgcaccagg tcggggtcgt ccaccacggc
     9721 gtagtagatg tcgttccccc acaccaggtg cgcaatgctg aacggtccct gcatgtcgca
     9781 catggaaagc cgaatcccgg aacgcagagc agggaaatcg gcaagctgtg caatcgcaaa
     9841 ctcctgaaac tcctctatcc tcgcccagag gcctccacgc aaatccaggg cgccggcgct
     9901 gacaatcttc gcaactgctt ctctgttgtt cagcggttct acccacggca tggcgtcata
     9961 cgtgagtttg atctcgcacc caaaaagaga cgggagtatc ccaacaccga aatttgctcg
    10021 aatcgtattc gcgcgatcat cacctacgag cgcactaccg tagatgggac ggagttcgtt
    10081 gagcaacatc agttcaggac acgcgtacgc ctcgtccagc ctgattcgtt cccagcctgg
    10141 cacttcatcc agcacgaatg ctaccggcaa ccggtccgat ttgtggaaaa ggcacacgtt
    10201 tttctggcgt tcgaccgatt ttgaactccg ctcaggatca aagacgtcgt tcaagcgcat
    10261 gaggtagggt tcgatattga cgttcatgct tttttctcac tgggtagaga aatgcgaaat
    10321 caccgcgtca acgtacgcgg aagcgaaccg ctcccgccat gcaggatctc tcatgttggc
    10381 agcgtcgttt tcgttcctga tgttcccgat ttccactaga attttggtgg gcgcggcatt
    10441 gtatttgagt atcgccggaa cccattcgcc accgccgctt cgccgtatga tgtacccgcg
    10501 tacaggtttg cgcccgtgaa cccctacgtt cgcgcttctg agacgttggc aaagcgcatc
    10561 agcaaacccc gaagacaacg cctgggaacg gagccgttcg ttgtacgagt aactcacggt
    10621 aggacgctcc gatacttcgt ggtagatcgc gtaccgcgtt cccgcgctgt accgtccctt
    10681 gctgtggcgg gcgctcggaa cgtacaccat acatccctgc atactggggt ggagcgcgtc
    10741 cgcgtgaaaa ctcgtgaaca caacccgctc gaggtccgta cccgtattga tcgcgcgccg
    10801 gacgtaggag ttcgccaggt accatcgaag attggctgat gtgacaacgt ccgcgttggg
    10861 gtatttcggc gtggtgagga ggtgctcctc ccgattgtcc ggtattgtag cccgctccga
    10921 aggcgtatgc cccgtccgtg cgtcctcgat ggttacgtac acggttgcct gtgttcgttg
    10981 ggcaaattgc ctcatcacac gacacattat gtcgtacacc acttcgtttt ccgtcagacc
    11041 tcgcggtccg atagcgcccg ggtcgtgccc cccgtgaccc gcatcaagaa cgatcgtaac
    11101 cccgcgtagc gcctcgccac gtgggcggac cggctctgca gcgcgtgcct ccgccttttc
    11161 gacgcggatt tcctcttcca cgcgggcaac cgccgtgtct tcctcacgct cggcaagccg
    11221 cgcggggtcg ccctcgggcc tccactcggg gctcaacaac ccgatgggaa tcttgataag
    11281 ggcgttaacc ggaagtttgc gtggatccct gaaaccggac ctgcgaagaa cgacctcgga
    11341 gaggtgcagg acctctgcta cttcgaccac ccccgtgaag cggatgacaa cgtcggagta
    11401 aagcgcctgg cctcgctgca gacggtaccc cgcataaccg cccagccggt cccccgaata
    11461 cacaaggggc ggatttctcg tcccccagta gacgtaccat tttgccgcat cctgtgcccc
    11521 gtcctctccc ttttccatca gtgcggtcca cgcagaaagc agtcgcttcg gaatcagaag
    11581 tcgctggccg aggttgaccg catcccgcct gctacgggag gtgagggcga gagggtaatc
    11641 cctggtttcc tcggaaatgt cctcctccgt cataagggcc cagcttccca gcagatttcg
    11701 cgggatgaca atgacttcac ccttcgccac atcatttgag cgtttccccg aagcgcgctt
    11761 aaggaggggc cagttttcgc cggttcccgt aaaccagagc gagacccgag aaaggctttc
    11821 tatcgggaga gtgacaatgt gccgtcgcga gtcttcccgg atcacgtcag agaaaaacag
    11881 atgtgtcaga acggcggctc gatatccgtc tttcatcgca tccgcgggaa ttacaatcgt
    11941 tgcacccctg tcaaactcac ggatacgacg cgggtctttc aggacagctt cggcaagacg
    12001 gcccgtttcg acaccttctt tgccgatcgt cacgaggatt tcgtggttcc gagtgatgga
    12061 gacttcgccg actgtcccgg ttcggcgtat ctgcccggcg ttttccgacg aaccagtgaa
    12121 aaaccgggcg attgccgcga cattctcgcg ctcgtcggtt accacatgtt cgaggccgtc
    12181 ttgcgaaagg ctgtcctcag gaaacaaacg cagagcgacg attgccttgt aaggatcact
    12241 caatg
//
LOCUS       DAOSOO010000083        12106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5235_length_12106_cov_4.897270, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000083 DAOSOO010000000
VERSION     DAOSOO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5235_length_12106_cov_4.897270"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="PLT86_07520"
     CDS             complement(<1..1661)
                     /locus_tag="PLT86_07520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT36497.1"
                     /translation="MRDLVVTPFRNETSAHPRHHHDQTGEPMDDSPKDHCGVIAVYNL
                     DKKSNRSVAPIVSSALLDLQHRGQLGAGMTSFDPGRTHLLKTHKDTGSVHEVFQLGHP
                     LEAKRLLEDYRGCAAIGHVRYATSGRDQAEYAQPFERQHSRRWKWFSLAFNGNLANYS
                     DLRDHLTKTRGYHMVYNVDTEVLVHHVAYALRGEKKPFLVTVFSELTCDLDGAYNIVF
                     INADGDLVAARSPLGIRPLCYGIIDGMFVAASESVALTNMGIEDFVDVEPGEMVWVDG
                     ETRTVHVDRFTQPRAHAHCFFEWVYFAHVTSVLEKHAVYDVRWRLGASLASREKLSMD
                     ADCVVVAVPDTAKPIGDAFGFTLGIPSLEGIVRNRYVGRTFIEGEDRAGKVRRKYTLV
                     TSVLRGRRVFLTEDSLVRATTLRGLVDRARTQGGAKEVHLRIGSPPVIAPCFYGIDMS
                     TLGELYAPKFLKPGYSDEELDRVCDEMARQLGADSLRYLPIGDLAACVGLPHDHLCTG
                     CVTGRYPTKAGERIFAKEQARQSSMPAGRSYEVTAVDHVAADEGH"
     gene            1856..3247
                     /gene="rimO"
                     /locus_tag="PLT86_07525"
     CDS             1856..3247
                     /gene="rimO"
                     /locus_tag="PLT86_07525"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT36498.1"
                     /translation="MKKIPFQLDATHATGNPKTRKGRTGRSIAMIPLGCAKAQVDAEQ
                     MLGALASAGYRIVHNPKRAEAVVINTCGFIQAAKEESLQTIFESLKLKKTHGVRTVAI
                     TGCLAERYAAELTKEIPEADIVLPWSQENELVHRLDASLGIHRGEEPQCGERILLNPG
                     HWAYLRISEGCNHMCAFCSIPAIRGRHVSVPLADLVAETERLAARGVREVNVVAQDTT
                     LWGIDLYGKPSLPLLLKSIAKVPGIRWVRLLYTHPAHITDELARAFSESEVLVPYLDM
                     PIQHINNVVLKRMNRIVGRAQIERIIHEFRTKIPHMVLRTTVIVGLPYETDRRFRELV
                     DFVEETKFDRLGCFIFSPEEGTEAARMRNRAPQSVAAERQAIIMEAQRAISRNMNKAR
                     VGKEYEVLTERRTGNSVIARSYAEAPEVDGVIRVRGSRLPAPGEWSRVRITKAFDYDL
                     LAAPVGAPSGMSG"
     gene            3364..4503
                     /gene="dusB"
                     /locus_tag="PLT86_07530"
     CDS             3364..4503
                     /gene="dusB"
                     /locus_tag="PLT86_07530"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT36499.1"
                     /translation="MNPGALDYQLDSAIVTGLAALPAVPPAASRVVRIGSVPIYGHAV
                     LAPMSGVCEQPFRWICRRAGASAVYTEFVSSDGLVRNNRHTERMLAFSEDERPVAAQL
                     FGSDPSVVFDAVQKIENMGVDIIDVNFGCPVRKVVKRDAGSALLKNPELLGRVLRAAV
                     DAARRVPVTAKIRSGWQSVNAREIATIVEANGAAAVAVHGRTQAMGYSGKADWGVIRA
                     VKETVTIPVIGNGDVFSPDAAVQMLNQTGCDLVMVARGAQGAPWLFAQINAAFAGELP
                     PNVEWAQRVEIAAMHLALLLRQRTELIAVRSFRKHLGFYTRGAPDSATFRREVFRCDS
                     ASEVLARMNEYAEFLAQFDGSVPKSRWGHTCNTQGPLTTSTEYDT"
     gene            4500..>5035
                     /locus_tag="PLT86_07535"
     CDS             4500..>5035
                     /locus_tag="PLT86_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HOT36500.1"
                     /translation="MTPDRIRTLLEQVRDGSLSTEKAMETLRAFPFEDLGFAQLDHHR
                     ALRKGFPEVIFCESKTVEQVVAIADRIVSHGADLLATRVTAAQGEALCAAIPQARYNL
                     TARTVALRQSNQPPPTRGEILVVCAGTSDLPVAEEAIETAEAMGHPVSLLADVGVAGI
                     HRLLSAQDRLFAASVIIVV"
     assembly_gap    5036..5135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5183..6037)
                     /locus_tag="PLT86_07540"
     CDS             complement(5183..6037)
                     /locus_tag="PLT86_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT36501.1"
                     /translation="MAYRLPDFEYKARSRLVDLLEELGTCTATEAARMVFNSTSIPAS
                     LAESLLEALTRSDQRFGRTPGGQWFLARKPVRSALRGATFVVLDVETTGTQPPAHRIT
                     ELGAVRVRESQIVNEFCSLINPEREIPLEITRLTGISNSMVADKPTALQLLPDFAEWL
                     GDDVIVAHNAPFDRGFIDAQWREVFGMATGNRWLCTVRIARWLYPEIHSRSLGPLCRH
                     LGIPIGRQHRAGDDARATAMVLLREIDDLSARGINDFDALDAVVAPLNAHPAKKRSRR
                     RMVPVEQT"
     gene            complement(6059..6664)
                     /locus_tag="PLT86_07545"
     CDS             complement(6059..6664)
                     /locus_tag="PLT86_07545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT36502.1"
                     /translation="MAPELGQHGSIPRETPDKTRAHRARLFTIANGISLLRVALLPWV
                     LFLISRPPGSHTVELIGLAVVIALTDFFDGFVARRTGQVSHYGRIIDPVADKICLVLA
                     SWWLTVYRGLPVWIPVLVVVRDAVILGGSFVLLRRHDVVLPSNQLGRVTTFVLTITLF
                     AYLIAWEAPQRLLVWLSGALVVATFVSYVRIGWIIFRRFDT"
     gene            complement(6664..8403)
                     /gene="recN"
                     /locus_tag="PLT86_07550"
     CDS             complement(6664..8403)
                     /gene="recN"
                     /locus_tag="PLT86_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT36503.1"
                     /translation="MLEELSVQDFALIERVSLTFSPRFNVITGETGAGKSILVGALGS
                     LLGMRTGAEMVRSGCEKATIEARFRLNRESAVRDLVLEFGGEIEDDTVVIRRLLGSDG
                     RTRVWIGGVSVSVKTLRDVASRLVDFHGQHDHQLLLSPAEHCAILDSFAGTQPLVASL
                     REKLSKLKQLRGQRAALLERQTQSAALRDSEEFERRELDEIDPAPDEYESLLAEQRVL
                     ENAERLGTLLASVCNLLSESDDSVTTRLGQARRWVQEASSIDERLAEEISGDYEVLEA
                     LAQEISARCAARVESLEADPGRLDYVSNRLAQLKRLIKRYGSLENAVTRRKELELRVD
                     ADAETAEAIDRLAEEIRCEHREMANLALELSRRRTEAARRLADEVSASLASLGMERAR
                     FEADMVPFSADEVDCLISDAVEYDGQARNVGDSGAEAVEFLIAPNSGEPLKPLARIAS
                     GGEISRVMLAIKSVLAEHDPVSSMVFDEIDAGISGRIGDAVGERMRALAEHRQIIAIT
                     HLPQIAARADHHIVVEKEEARGRTLTLARAAEGEERVLVLAELLGGGEARSTAIEHAR
                     AILNGSEDRGATR"
     gene            complement(8425..9294)
                     /locus_tag="PLT86_07555"
     CDS             complement(8425..9294)
                     /locus_tag="PLT86_07555"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT36504.1"
                     /translation="MAIGSVGVVANAEKPNTRDIIRRILKSGERLGFSVVVDAEVADA
                     LGCGSVGVAGEELSKCAELIVACGGDGTILRAARITMGAPRPILGVNLGRLGFLAEAS
                     PEKLEQALEEIDRGDFVTEPRMTLTATVMGTGETVHALNDITIAKSNQSRIISLEMRE
                     AGNWVNTYIGDGLILATPTGSTGYALAAGGPIVKPLAELIVAAPICPHSLTVRPIVFP
                     DDAVLDIRVAGAAESAILLTADGQPTVPLPSGEEVRVRRGAKDVQLVRLASSSYYDTL
                     RQKLHWGLDKNLR"
     gene            complement(9326..11236)
                     /gene="dxs"
                     /locus_tag="PLT86_07560"
     CDS             complement(9326..11236)
                     /gene="dxs"
                     /locus_tag="PLT86_07560"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT36505.1"
                     /translation="MPGLLETIDSPLDLRRLPEDQLPELAREIRSLILDVISRNSGHF
                     ASSLGVVELTLALHRVFNTPRDKIIWDVGHQAYVHKIITGRREQFHTIRKKGGLSGYP
                     RREESPYDVFGVGHASTSISAALGLATGRDLRGEDFHVVAVIGDGSMTGGMAWEAINN
                     AGASGRDMLIVLNDNRMSISPNVGALARYFNEIITHETYNKLKADIWDFLGKIPNMGE
                     KVRVGISQLEKAVKSLLVPGIVFEKLGIRYFGPVNGQDVHALIETLEQIKDLKGPRLL
                     HVYTQKGKGVPFAENDPYKWHAGGQFDPRNGEALKKSSPVSYTDVFGKTLTKLCDSHP
                     NVVGITAAMASGCGLKHLADKYPYRFFDVGIAEQHAVTFAGGLALEGTKPVVAIYSTF
                     LQRAYDQIVHDIALQRLPVAFVLDRGGLVGDDGATHNGVFDLSYLRHIPNMVIMAPKD
                     ELEMQRMVLTLVEYNEGPIAMRYPRGCGPGAAIVEEPEALEPIGIGTWEVLAPGTEMV
                     VLAIGSMVYPALEATQTLREEGISIEVVNARFAKPLDDGMMEELVRRHRVWVTVEENV
                     LAGGFGSGVMEALECRGLLSSVSLSRLGIHDRFSDHGTREEVLHDEGLDAVSLSEAFR
                     VIRARTLEMKVG"
     gene            complement(11264..11536)
                     /gene="xseB"
                     /locus_tag="PLT86_07565"
     CDS             complement(11264..11536)
                     /gene="xseB"
                     /locus_tag="PLT86_07565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT36506.1"
                     /translation="MTETEKPLNFEQAVKRLEEIVHALDDQTLPLDAALALFEEGILV
                     SRQCAAHLEAARARVEKLVEQAPGVFSLEAFDQETSAVDADDEDEE"
     gene            complement(11688..>12106)
                     /locus_tag="PLT86_07570"
     CDS             complement(11688..>12106)
                     /locus_tag="PLT86_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36507.1"
                     /translation="ARLHTNIVFRHCGTVRQSTIMELTAGIANPVDAGAGLAEGKIRA
                     LEEETDKADGCCPPLRSFVLPAGSSAACALQVARAVCRRAERAIVRLGTEGELPKNTL
                     PFINRLSDLLFAYARWANVRAGAAEELWEAPGKAAH"
BASE COUNT         2430 a         3665 c         3438 g         2473 t
ORIGIN      
        1 caatggcctt cgtcggcagc aacgtgatcc accgcagtca cttcataact gcgaccggcc
       61 ggcatgctgc tctggcgagc ctgctccttc gcgaaaattc gttcaccggc ctttgtcgga
      121 tacctgccgg taacgcatcc ggtgcacagg tgatcgtggg gaagaccaac gcacgccgcg
      181 agatcgccaa ttggaaggta gcgaagcgag tcggccccga gctggcgtgc catctcgtcg
      241 cacactctgt ccagctcttc atccgagtaa ccgggtttga ggaacttcgg ggcatacaac
      301 tcgccaaggg tggacatgtc aataccgtag aagcatggcg cgattaccgg tggcgaaccg
      361 attcggaggt gcacttcctt cgccccgccc tgtgttcgtg cccggtccac caggccgcgg
      421 agtgttgtcg cgcgcacaag gctgtcttcc gtaagaaaca ccctacggcc tcgaagcacg
      481 ctggtcacga gggtgtactt gcgtcttact ttgccggcgc ggtcttctcc ctcgatgaac
      541 gtgcggccga cataccggtt tcggacgatt ccttcgagcg acggaatgcc cagcgtgaaa
      601 ccgaaggcat caccgatggg cttcgccgtg tctggaacgg caaccactac gcaatcggcg
      661 tccatggaga gcttctcgcg tgatgccaga gacgcgccga gtcgccaacg aacgtcgtac
      721 accgcgtgct tctccagcac agacgtcacg tgcgcgaaat atacccattc aaagaaacag
      781 tgggcgtggg cgcgaggttg cgtgaaacgg tccacgtgaa ccgtccgggt ttccccgtca
      841 acccagacca tctcccccgg ctcgacatcc acaaaatcct ctattcccat gttcgtaagc
      901 gcgacacttt ctgacgcggc aacaaacatc ccgtctatga tgccatagca aagcggcctt
      961 atcccgagcg ggcttcgtgc agcaacgagg tctccgtctg cgttaataaa cacgatattg
     1021 tacgcgccgt cgagatcgca cgtcagttcc gagaacaccg taacgagaaa gggcttcttt
     1081 tcacctctca acgcgtacgc aacgtgatgg acgagcacct cggtgtcaac gttgtagacc
     1141 atgtggtagc cgcgcgtttt cgtgagatgg tcgcgcaagt cgctgtagtt cgcgagattg
     1201 ccattaaacg cgagcgagaa ccatttccag cgccggctgt gctgtcgctc gaagggttgc
     1261 gcatactccg cctgatcccg gccgcttgtt gcataccgca cgtggccgat cgcggcgcag
     1321 ccgcggtaat cctccaacag acgcttggcc tcaagcgggt gacccaactg gaacacctcg
     1381 tggacggagc cggtgtcctt gtgcgtcttc agcaggtggg tacgcccggg gtcaaatgaa
     1441 gtcattccgg ctccgagttg gccgcggtgt tggaggtcca gcaacgcact tgaaactatg
     1501 ggcgccaccg agcggttgct ctttttgtcc aggttgtaga ctgcaatgac accgcaatgg
     1561 tctttcgggg agtcgtccat cggttctccg gtctgatcgt ggtgatgccg aggatgtgcc
     1621 gaggtttcgt tacggaaagg cgtgacaacg agatcgcgca tcgtattttc cgcttcttat
     1681 ccgaagatcg agcacaggca aacgagggaa cccgggatac agaatatacg gacgaagccc
     1741 cctttgcccc tacggcgccg tggacatcgg ttttctgtta ctgggaagcg gtacgtgcgt
     1801 atcttgtacg aaccacacgt ttgagtctgc cgaagcataa tcacgagggc tgccgttgaa
     1861 aaagatcccg tttcagttag acgccacaca cgccaccggc aacccgaaga cacggaaggg
     1921 tcggaccggt cgttctatcg caatgatacc gctcgggtgc gcaaaggctc aggtcgatgc
     1981 agaacaaatg ctgggcgcgc tcgcctcagc cggttacagg atcgtgcaca atcccaaaag
     2041 agccgaagca gtggtcatca acacctgtgg gttcattcag gccgcgaaag aagagagtct
     2101 ccagacgatt ttcgagtccc tgaagctgaa aaagacacac ggtgttcgaa cggtggcaat
     2161 cacgggatgt ctggcagaac ggtatgcggc agaactcacc aaggagatac ccgaagctga
     2221 tatcgtgctc ccgtggtctc aggagaatga actggtgcac cggctggacg cttcgctggg
     2281 catccatcgg ggggaggagc cgcagtgtgg cgaacggatt cttctgaacc cggggcactg
     2341 ggcatatctg cgcatttctg agggttgcaa ccacatgtgc gcgttttgct ccatcccagc
     2401 gattcgtgga aggcacgtca gcgttcctct cgcagatctg gtcgccgaga cggaacgtct
     2461 ggcggcgcgt ggtgtccgag aagtgaatgt ggtggcgcag gataccaccc tctggggtat
     2521 cgatctgtat ggaaagcctt cccttcctct gcttctgaaa agcatagcga aggttccggg
     2581 aatacggtgg gtgcgcctgc tctacacaca tcccgctcat atcacagacg aactggcccg
     2641 cgcgttctcc gagtcagaag tattggtgcc gtatctcgac atgccgattc aacacatcaa
     2701 caacgttgtt ctgaaacgga tgaaccgcat cgtcggtcgc gcacagattg aacggatcat
     2761 acacgaattc cgcacaaaaa tcccgcacat ggttttgcgg actacggtga ttgtcggcct
     2821 tccctacgaa acggatcgac gtttccgaga actcgtcgat tttgtcgaag agacgaaatt
     2881 cgacaggctg ggttgcttca tcttttcgcc cgaagaagga actgaagcgg cccgaatgcg
     2941 aaaccgcgcg cctcaatccg tggcagcgga acggcaggcg attattatgg aagcacagcg
     3001 cgcgatctca cgcaacatga acaaggctcg ggtcggtaag gagtacgaag tcctcacaga
     3061 gcgccggaca ggcaattcag tgatcgcgcg cagctatgcc gaagcgccgg aagttgacgg
     3121 cgtcatacgt gtgcgcggct cacgtcttcc ggcgccgggc gagtggtcac gggtacgaat
     3181 taccaaggct ttcgactacg atctgctcgc cgcgcctgtg ggtgcgcctt ccggtatgag
     3241 cgggtgagcc tatcgccgtg gaaccgattt ccgttccaca tggttcgata cgatgttgga
     3301 ggttgcgtct ccggggcatt ttcctgctct gcgaggccac cgtcttgagc aggattcgac
     3361 gcgatgaatc cgggagcgct tgactatcag ctggattccg ccatcgtgac cggtttggcg
     3421 gcgctgccgg ctgttccccc ggcggcatca agggtcgtca ggataggttc cgttccgatc
     3481 tatgggcatg cggtacttgc tcccatgtca ggcgtgtgtg aacaaccctt tcgctggatt
     3541 tgccggcgcg cgggagcttc ggcagtctat accgagtttg tgagctccga cggtcttgtt
     3601 cgtaataatc gccatacgga acggatgctc gccttctcgg aagacgaacg gccggtggcc
     3661 gctcagttgt ttggctccga cccctccgtt gtatttgacg cagtgcagaa aattgagaac
     3721 atgggtgtcg acatcattga cgtgaacttc ggatgcccgg tgagaaaggt ggtgaaaagg
     3781 gacgcaggct cggcgctcct taagaacccg gagctcctcg gcagggttct ccgagctgcc
     3841 gtggatgcgg cccggagggt acccgttact gcaaagattc gttccggctg gcaatcggtg
     3901 aatgcgcggg aaatcgcaac gatcgtggaa gccaacggcg ccgcggctgt cgccgtgcac
     3961 ggccgcaccc aggccatggg gtacagcgga aaagcagact ggggtgtcat ccgcgcggtg
     4021 aaggaaaccg tcaccatccc cgtcatcggg aacggagatg tcttttcgcc cgatgcggcg
     4081 gtccagatgt tgaatcagac gggttgcgac ctcgtgatgg tggcccgtgg cgcacagggc
     4141 gctccctggc ttttcgcgca aatcaacgcc gcgttcgcgg gggagttacc accgaacgta
     4201 gaatgggcac agcgggtgga gatcgcagcc atgcacctcg ctctcctgct taggcagcgt
     4261 acggaactga tcgcggtaag gagttttcgc aagcacctcg gtttctacac ccggggagca
     4321 cccgattccg cgaccttccg tcgtgaagtg ttccggtgtg attccgcgag tgaagttctg
     4381 gctagaatga acgagtacgc ggaatttctc gctcagttcg acgggagcgt gccgaagtcg
     4441 cgatggggtc acacgtgtaa tacgcagggt cctttaacca cctccacgga gtatgataca
     4501 tgacaccgga ccgtatccga accctccttg aacaagtgag agacggttcc ttgagcaccg
     4561 aaaaagccat ggaaaccctc cgggctttcc cattcgaaga cctcggtttc gcgcagctgg
     4621 accaccacag agcgttgcga aagggattcc ccgaggtcat tttttgcgaa tccaagactg
     4681 tcgaacaggt tgtggctatc gcggaccgga ttgtctctca cggggcggat cttctggcta
     4741 ctcgtgtgac cgccgcgcaa ggggaggcat tgtgcgcggc aattcctcag gcgcggtaca
     4801 accttaccgc tcggacggtg gctcttcggc aatcgaacca gccacccccc acgcggggag
     4861 agattctggt tgtatgcgcc ggcaccagcg atctgcccgt cgcggaggag gcgatcgaaa
     4921 ccgcggaggc gatgggacac cccgtgtcgc tgctcgcgga cgtgggtgtc gcaggtattc
     4981 accggttgct cagcgcgcag gatcggctgt ttgccgccag cgtgatcatt gtcgtnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgca gtgacaccag tcctggtgac
     5161 gtacaagaac cgcaggaaat cgtcaggttt gctcaaccgg aaccatccgc cggcgggacc
     5221 tttttttcgc ggggtgcgca ttaaggggag ccactaccgc gtccagagca tcaaagtcat
     5281 tgattcctcg ggcagaaagg tcgtcaattt cccggagaag caccatcgcg gtcgcccggg
     5341 cgtcatctcc tgcgcggtgc tgtcgtccga tgggaatgcc gagatggcgg cacaacgggc
     5401 caaggctcct gctatggatt tccgggtaca accagcgggc aattcggacc gtacacagcc
     5461 accgattccc tgtcgccatc ccgaacacct cgcgccactg cgcgtcgatg aagccgcggt
     5521 caaatggcgc attgtgtgcc acgattacgt cgtctccaag ccactcggcg aagtcgggca
     5581 gtagttgcag tgcggtgggt ttgtcggcca ccatcgagtt tgatatcccg gtcagccggg
     5641 tgatttccaa tggaatctcc cgttcgggat tgatcaggct gcagaactcg ttcactatct
     5701 ggctttccct gacacgaacg gcaccgagtt cagtaattcg gtgagccggc ggttgggttc
     5761 cggttgtttc cacgtcaagc acaacgaagg tcgcccctcg caacgcactg cgaacaggct
     5821 tccgagcaag gaaccactgc ccgcccggag ttcgtccgaa gcgttgatcc gatcttgtga
     5881 gcgcttccag caacgactcg gcaagcgagg ccggaatgga ggttgagttg aacaccattc
     5941 gagccgcttc ggtcgccgtg cacgtgccga gttcctcaag aaggtcgacc agacgggagc
     6001 gcgccttgta ttcaaagtcc ggcagacggt aggccaagga agagcccctt tgtggcggtc
     6061 aagtgtcaaa gcgccggaaa atgatccacc ctattcggac gtacgacacg aatgtcgcca
     6121 ctacaagcgc gccgctcaac cagacaagaa gccgttgagg tgcctcccat gcgatgagat
     6181 aggcgaacag ggtaatcgtg agcacgaagg tcgtgacccg gcccaactgg tttgacggca
     6241 acacgacgtc gtgtcgccgc agcaacacga agctcccccc gagaatcacc gcgtctctca
     6301 ccaccaccag aacaggtatc cagacgggta accccctgta caccgtcagc caccacgagg
     6361 cgaggaccag gcagatcttg tcggcaaccg ggtctatgat gcgtccgtaa tgtgaaacct
     6421 gtccggtgcg ccgcgcgacg aagccgtcaa agaagtcggt cagagcaatc acgacggcaa
     6481 gcccgatcag ttccaccgtg tgggaaccgg gaggacgcga aattaggaat aacacccatg
     6541 gaagcaacgc gacgcgcagg agactgatgc cgttggcgat ggtaaatagg cgcgcacggt
     6601 gcgcccttgt tttgtcgggt gtctctcgag ggatcgaacc gtgttgcccg agttccggcg
     6661 ccattaccgc gtcgccccac gatcttccga cccgttcagg atcgcacgtg cgtgttcgat
     6721 ggcagtactc cgagcctccc ctccgcccag caactccgca agcaccagca cgcgctcctc
     6781 tccctccgct gcgcgcgcga gtgtcagcgt tcgcccacga gcttcttcct tttccaccac
     6841 gatgtggtgg tcggcgcgag cagcgatttg tggaaggtgc gtaatcgcga tgatctgccg
     6901 gtgttccgca agagcgcgca ttctttcccc gacggcatcg ccgattcgtc ccgatatccc
     6961 tgcgtcgatc tcatcgaaca ccatggagga aaccgggtca tgctcggcga gaacgctctt
     7021 gatagccaac atcacacgag aaatctcacc tccggaagcg atacgggcga gcggcttcag
     7081 cggttctccg gaattcggcg cgatcagaaa ctccaccgcc tcggcacccg aatcgcctac
     7141 gttccgggcc tgtccatcat actcaacggc gtccgaaatg agacagtcca cctcgtctgc
     7201 ggagaaagga accatgtccg cttcaaagcg cgcacgttcc attcccagtg acgcaagcga
     7261 cgcgctgacc tcgtcggcta ggcggcgtgc cgcctccgtc ctcctgcgtg atagctccag
     7321 ggccagattc gccatctcgc ggtgttcgca acggatttcc tcagccagcc gatcgatcgc
     7381 ttccgccgtt tccgcgtcgg cgtcgactct cagttcgagt tcttttcttc gcgtgaccgc
     7441 gttttccagg ctcccgtatc gtttgatcaa gcgtttcagt tgagcaagcc ggttggatac
     7501 gtaatcgagc ctcccggggt cagcttccag agactccacc cgcgctgcgc accgagcgga
     7561 gatttcctgc gcgagagctt ccagcacttc gtaatcgcca ctgatttcct ccgcgagcct
     7621 ttcgtctatg gaggacgcct cctgaaccca cctccgcgct tgccccaggc gggttgtcac
     7681 ggagtcgtcc gattcgctca gaaggttgca cacgctggca aggagtgttc cgaggcgttc
     7741 cgcattttca agtacgcgct gttcggccag aagggactcg tattcgtcgg gcgcggggtc
     7801 tatctcgtcc agttccctgc gttcgaactc ctctgaatcc ctcagcgcgg cactctgggt
     7861 ttgccgttca aggagagctg cccgttgccc acgcagttgt ttcagcttcg aaagcttctc
     7921 tcgcaacgag gccacaagcg gctgcgtacc cgcgaaggag tccagtatcg cgcagtgttc
     7981 agccggcgaa agaagaagct ggtgatcatg ttgtccgtga aaatccacga ggcgggaagc
     8041 cacgtcacgt aacgtcttta cggaaaccga aacaccccct atccacacgc gcgtccgccc
     8101 atcgctcccc agcaggcgac gaatgaccac cgtatcgtcc tcgatctccc ccccgaattc
     8161 gagcaccaga tcgcggacgg cactctccct gttcagccgg aaccgtgctt caatggttgc
     8221 cttctcgcat ccggagcgca ccatctccgc gcccgtacgc attccgagca gggaaccgag
     8281 cgctcccaca aggatcgact tgcccgcacc tgtttcaccg gtgatgacat tgaaacgggg
     8341 agagaaggtc agggatacac gctcaatcag cgcgaaatcc tgaaccgaca gttcttcaag
     8401 catcggcccg ctctggcctc gtggctaccg aaggttctta tcgagtcccc aatgcaactt
     8461 ctgccgcagc gtgtcgtagt acgacgacga ggccaaccga acaagctgaa cgtctttggc
     8521 ccctcttcgc acacgcactt cttctcccga gggcaggggt accgtgggct gcccgtcagc
     8581 ggtgagcaat atcgcgcttt cggcagcccc tgccacgcga atgtcgagta ccgcatcgtc
     8641 gggaaacaca atcggacgca cggtaagtga gtgggggcat attggtgccg cgacgatgag
     8701 ctccgccaga ggcttgacga tgggtcctcc tgcggcgagc gcgtaccccg tggaccccgt
     8761 gggtgtcgca agaatcaacc cgtcgccgat gtacgtgttc acccagttgc ccgcttcgcg
     8821 catctccagc gagatgatcc gggattgatt gctttttgcg atagtgatgt cgttcagagc
     8881 atggacggtt tcgccggtgc ccataactgt tgcggtgaga gtcattcgtg gttcggtaac
     8941 aaaatcgccg cggtcaatct cctcaagggc ctgttccagt ttctccggtg aagcttccgc
     9001 aaggaatccg aggcgcccca gattcactcc cagtatggga cgcggggcac ccattgtgat
     9061 ccgtgcagcc cgcaagatcg tcccgtctcc gccgcaggca acgatcaact cggcgcattt
     9121 tgacagctcc tcgcctgcca cgcccacgga tccgcacccg agggcatcgg cgacctctgc
     9181 gtcaacaact accgaaaacc cgagccgttc gccggattta aggatccgcc ggatgatatc
     9241 acgcgtgttc ggtttctccg cgttcgccac aactccgacg gatcctatcg ccatccccta
     9301 ccccgcattc gcgaaccggt acaaatcacc ccaccttcat ctccagcgtc ctcgccctga
     9361 tcacgcggaa tgcctctgag aggcttaccg catcgaggcc ttcgtcgtga agcacctctt
     9421 cgcgagtacc gtggtccgaa aaacgatcgt gaatgccaag tcgactcagt gaaaccgatg
     9481 aaagcagtcc gcggcattcc agagcttcca ttacgcccga tccgaaaccc ccggccaata
     9541 cgttttcttc aaccgtcacc cagactctgt gcctgcggac aagttcttcc atcatcccgt
     9601 catccagagg tttggcaaac cgcgcattga cgacttcaat cgaaataccc tcttctctga
     9661 gggtttgagt ggcctcgagc gcgggataca ccatgcttcc tattgcaagc acgaccatct
     9721 ctgtccccgg cgcgagcacc tcccacgtcc cgatcccgat cggttcaagt gcctctggtt
     9781 cctcaactat ggccgcaccc ggaccgcaac cgcggggata cctcatggct atgggcccct
     9841 cattgtattc gacgagcgtg agcaccatcc gctgcatttc cagctcgtct ttaggtgcca
     9901 ttatcaccat gttcgggatg tggcgaaggt agcttaagtc gaaaacgccg ttgtgcgtgg
     9961 ccccgtcatc ccctaccagc cccccgcggt caaggacgaa cgccaccggg aggcgttgca
    10021 gcgcgatgtc gtggacgatc tgatcataag cccgttgcag aaatgtgctg tagatggcga
    10081 caacgggttt tgtgccctcc agcgcgagcc cgcccgcgaa cgtcactgcg tgttgttccg
    10141 caatgccgac atcgaaaaaa cgatacgggt acttgtcagc aagatgcttg agcccgcacc
    10201 ccgaggccat cgccgcagtg atcccgacaa cgtttggatg tgaatcacaa agcttcgtca
    10261 acgtcttgcc aaacacgtcc gtgtacgaca caggtgagct tttcttgagc gcttctccat
    10321 ttctcggatc aaactggccg ccggcatgcc acttgtacgg atcgttctcc gcgaacggaa
    10381 cacccttccc cttctgcgtg tacacgtgca gcagacgcgg acctttcagg tccttgatct
    10441 gctccagagt ttcgatgagc gcgtggacgt cctgtccgtt gacagggccg aagtaccgaa
    10501 tcccgagctt ctcgaagaca atcccgggca caagcagcga tttcaccgcc ttttccagct
    10561 gacttatccc aacgcggact ttttcgccca tgttcggtat ttttccgagg aaatcccaaa
    10621 tgtctgcctt gagtttgttg taggtctcgt gcgtgatgat ttcgttgaaa taccgcgcca
    10681 gcgctccgac gttgggcgaa atagacatgc gattgtcgtt caagacgatg agcatgtccc
    10741 ggcctgaggc acccgcgttg ttgatcgctt cccatgccat gccgccggtc attgatccgt
    10801 caccaatgac ggccaccacg tggaaatcct cgccccggag gtctctgccg gtagccaggc
    10861 cgagcgccgc gctgatgctc gtggaggcat ggcctacccc gaatacatcg taggggcttt
    10921 cctctcgccg gggatagccg ctcaatcctc cctttttccg gatggtgtga aactgctcac
    10981 gccgaccggt gatgatcttg tggacgtacg cctggtggcc cacgtcccag atgattttgt
    11041 cgcggggagt gttgaacaca cggtgcagtg caagcgtaag ctccaccaca ccgaggcttg
    11101 atgcgaaatg accgctgttc cgcgaaatca cgtccagaat gagtgagcgg atctcgcgtg
    11161 ccagttcagg caattggtct tccggcaacc gcctgaggtc aaggggtgag tctatggttt
    11221 ccaacaagcc gggcatctgg tctccagaaa ttctcgcagt gttctattcc tcgtcttcgt
    11281 catccgcatc aacggcggac gtctcctggt cgaacgcttc cagactgaac acaccgggag
    11341 cctgttcgac gagcttttcc acccgcgctc tcgccgcttc gagatgtgcc gcgcactggc
    11401 gggaaaccag tatcccttcc tcgaaaagag ccagcgctgc gtcaagcggc agcgtctggt
    11461 cgtcaagcgc gtgaacgatt tcctcgaggc gctttacggc ctgttcgaag ttcaaaggtt
    11521 tttccgtttc cgtcacggca acacctcggg gcataacggc gggcaacccg gcaaaggccg
    11581 cccaggatgg gtgatttgtt aacgtaaatg tacaaccgca gctgtccaac ggcgaggaca
    11641 agtcgtccaa gtcgttgggc acttcgaaac ggcctccgag gaagcactca gtgtgccgct
    11701 ttgcccgggg cttcccacag ttcttcggcg gcaccggcgc gaacgtttgc ccaccgcgca
    11761 taggcaaaaa ggagatcaga aaggcgattt atgaacggaa gtgtgttttt tggaagttcc
    11821 ccttcagttc cgagcctcac aattgcacgc tctgctctcc tgcacacagc tcgagcgacc
    11881 tgcagggcac acgcggccga agacccggca ggaaggacga aggaacgcag aggtggacaa
    11941 caaccgtccg ctttgtccgt ttcctcttcc aatgcacgaa tcttcccttc cgccaatccc
    12001 gctcccgcgt ccaccgggtt ggcaattccc gcggtcaatt ccatgatggt tgactggcgg
    12061 acggttccgc agtgccgaaa gacgatgttc gtgtggaggc gtgcgg
//
LOCUS       DAOSOO010000084        11952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5305_length_11952_cov_6.289989, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000084 DAOSOO010000000
VERSION     DAOSOO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_11952_cov_6.289989"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..745)
                     /locus_tag="PLT86_07575"
     CDS             complement(110..745)
                     /locus_tag="PLT86_07575"
                     /inference="COORDINATES: protein motif:HMM:NF039405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6308 family protein"
                     /protein_id="HOT36508.1"
                     /translation="MNDSLTLSPFARKINGCAAKLRCFRENAGYAYLAYQPITPWDRL
                     VPEDLAVTLAFNSNAGWRAFASLVAHAYEVDLSKLPEKALHETTPDERERIIELISEI
                     VKWPGFATSLATKVLHKKRPHLIPVLDNRAIFESYLDHRWPERRTVGDTVKDATKVRH
                     ALDALVADLNAPENAGTWQALSAVDPGLTRIEALDMVWWMHFRDLEPVKAP"
     gene            1199..1879
                     /locus_tag="PLT86_07580"
     CDS             1199..1879
                     /locus_tag="PLT86_07580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT36509.1"
                     /translation="MRVLDYGCGAGEIVKLLRARNIEAFGCDVYYDAADYSNTMDPAL
                     FGTLLRKMEDGRIPFADSAFDFVINNQVMEHVENLDAVLAEISRVLKPGGFVLSLFPD
                     KRVWREGHSGIPFFHWFPKGSRFRVYYALVLRTLGLGYFKEGKPRKKWSEDYCIWLDK
                     WTYYRSRKEIRETFAKHLAELNHIEVYWLHKRLGARRVIAAWLPAVIQRYVVWKLAGL
                     VLEARKAF"
     gene            complement(1949..2704)
                     /locus_tag="PLT86_07585"
     CDS             complement(1949..2704)
                     /locus_tag="PLT86_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT36510.1"
                     /translation="MVRKIVLCAAVVATAFFVGCGGEEKECVPVTVEAQTVEIITEDS
                     VTLVGTWFGVPNSRASIVCLPALTHSRGTWASTAQQFAASGWSVLTVDLRDQGDSQMP
                     VGASPVWVGNDTLHALAYYMKDVKAAVAFVRSKAPGAKVVVLGAGIAGYCGLHEAVAD
                     PTVAAVALFSPPESSRLTTPAIMAEYGDRPLLIMTELPAEAIRTTPRTLDSWIGRGSV
                     IEIKEQVPRPPLRLENKAAAVDFLNRWIKAAVR"
     gene            complement(2833..5517)
                     /gene="aceE"
                     /locus_tag="PLT86_07590"
     CDS             complement(2833..5517)
                     /gene="aceE"
                     /locus_tag="PLT86_07590"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOT36511.1"
                     /translation="MSETTRSGEPAGMNLAEKREWLDSLYYVLRSGGPERVEGLLQML
                     QNEARVAGVDVPFSASTPHINTIPPEKQPPYPGSREIERRIKSLVRWNAMAMVVRANR
                     ASSSIGGHISTYASAATLYEVGFNHFFRGRGNGYDGDQIYFQGHASPGIYARAFLEGR
                     LTEEDLESFRQELRPGGGLSSYPHPWLMPEFWEFPTVSMGLGPIMSIYQARFNRYLED
                     RGLKKDTGGKVWAFLGDGETDEPESLGAITLAAREKLDNLIWVINCNLQRLDGPVRGN
                     GHIIEELEAAFRGAGWNVIKVVWGSEWDELIAADRDGILVRRMGEVVDGEYQKYSVEG
                     GAYIRKHFFGADPRLLKMVENYSDEHLQKMRLGGHDPEKVYAAFKAAVEHKGHPTVVL
                     ARTIKGYGLGEAGEGKNVAHQQKKMNEKELSEFRSRFGIPISDEDLATAPFYRPPDGS
                     REIEYMLERRRALGGFAPSRKPRNAPLPPPDEDLFAEFAKGTEDRGVSTTMVFVRLLT
                     KLLRDPEWGRLIVPIVPDEARTFGMEALFRQSGIYSHVGQLYDPVDRDNLLYYKEARD
                     GQILEEGITEAGSMASFIAAGTAYATHGVNTIPFFIFYSMFGLQRVGDLVWAAGDIRA
                     RGFLIGGTAGRTTLAGEGLQHQDGNSHLLAYPVPNLIAYDPAFAFELAVIIRDGMRRM
                     YHEQESVFYYVTVENENYPMPAMPAHVDPAAILKGMYKFRPASLPASEAKANLFGSGT
                     ILNEALKAQHLLEDVYNVPTDVWSITSYKELYRDGLGAERWNILHPFDPPKEPYVSSV
                     LKQETGAFVMASDYVKALPASIDRWFPTPPVCLGTDGFGRSDGRRALRRFFEVNAEHI
                     TLSALTELTRQGKFAPDDLKKAMRDLLVNPDKPNPATS"
     gene            5778..6815
                     /locus_tag="PLT86_07595"
     CDS             5778..6815
                     /locus_tag="PLT86_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36512.1"
                     /translation="MLTLNRLVFFLTFVSASVSSGATRYAGDFLAVGVSPRATGMGSA
                     VTAIVGGPEASYWNPAGLAAGMTGAPVSVAMEHAERFGGVVKHDAVSAAIPLSRSVLG
                     VFLFRGAVDGIIFADSTTLVDPSLPLSESNMPDPSKTRTFSNADYVIHVATGHTVLRT
                     LRVGAGVKLIRRTIGGTAAFGYGVDLGAQWEPRPQIAVGILVRDATTTRVNWDNSRTD
                     VVYPTVHTGAAFGFPFPRFGGRLVVAGNSTVGSESGGYGGFAPWRVLNARNVANLGAE
                     YEWRETIALRLGSQNVRGMLGPGSAEVTAGMGLRGHLPWFSGVRRVGVDLAWIRHTLR
                     DSFRLGATVEW"
     gene            6839..8047
                     /locus_tag="PLT86_07600"
     CDS             6839..8047
                     /locus_tag="PLT86_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36513.1"
                     /translation="MKHVVSLVSTAGLCGVVACSSISSPTVYVRDPSYDFRGKTVGVC
                     VILDPVEQEALSDQRVVNRATSSGQTEGYASEMTEQPLEEDTGLLTPPKTVTSLQTKL
                     KHWRYYDEDFTLAMVDEVSAELKKRGFKVRVIPVPEGQVELVELAQRAQSDTCQILAV
                     VTVTVARSWNIRHQQTLRSTQDLSSSMRWVSYTGGLVLCNIAMFDIATNKVVWQHSQR
                     VVPLSRTVPVIAELYDNEVASTRFSSRPGLYQGWMYRQAMPRAVRLMFETAESGFIPL
                     PRGSESVIADTAPAPFASGCRVFVRPDSSSRLWFPATVIRDSAGVVSVQWADGIWEAY
                     GRRVEYPRSDVRPRDEFPEIVWVRTRDRLEFTPYRFVRVDVRGLVHVTRAGDMSPSTF
                     LPGRIGVVLE"
     gene            complement(8129..10225)
                     /gene="fusA"
                     /locus_tag="PLT86_07605"
     CDS             complement(8129..10225)
                     /gene="fusA"
                     /locus_tag="PLT86_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT36514.1"
                     /translation="MNEHSANTIRNIAFVGHGGAGKTTFVEAVLYTLGVTTRMGSVTD
                     GNTVSDYNADEQLRKFSLNATVLQFEAGAIKMNVIDTPGYTDFIGEAVGALRAVDNVA
                     VFINAQTGVEVGTELIWGYAAEFHKPALIVINACSREHADFQQAVDQAKDRFGSSVVV
                     CQFPVNQGEHFNQIVDVVTGKLYTYEPGGKGVAKISELSGELASRAQQMREALMEAVA
                     ESDEALLETYLEAGELEEDGFIAGLKAAIKNRMVYPVLASDAHHNVGTDRILEWIAEY
                     LPSPADMGPVEATVAGSDGKVQIQCSEADPLCAYTFKTVSEAHVGELSFVRVYGGKIT
                     QNIEVANVSQNASERIGQSFFMLGKDRTDAGRINAGDIGALVKLRTTHTGDTLCDKSR
                     QVLLPGVEWPKPNISFAVVPRTKGDEDKVANGLHRLHAEDPTFYHEVDGELNQTLIYG
                     QGELHLDVMVKRLAERFGVVVDLEKPKIAYRETIRKKAEAQYRHKKQTGGRGQFGDVH
                     LRIEPLERGAGYEFVDAIVGGVIPGKFIPAVEKGVLEAMESGIIAGCKVVDVRSTVFF
                     GSYHPVDSSEIAFKLAALNAFKNAMREAGPVLLEPIYKIEVTVPEEFMGDVMGDLSGR
                     RGKILGMDAKGPFQVIRALVPLANLFRYSTDLRSMTGGRGLHTREFDHYEEVPPDIMV
                     KIQEEFQKQKEAEEKD"
     gene            10584..11822
                     /locus_tag="PLT86_07610"
     CDS             10584..11822
                     /locus_tag="PLT86_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT36515.1"
                     /translation="MKFTSYQKKTLKALVLWSVIAAAAYAIAPRVPRLLRLASGMNLL
                     ELIGLLALILAIGEALGLLTQVILVGKRKPPVEGAMVGRIYRLVAVLAVLLGIAYGFG
                     KLGTIGAFFAMFGGMLLGWSLQAPVSGIAAWVLVSLKRPFRPGDRIQFPNLGLVGDVH
                     DTGIMYTMLNQVGGTVGSEEAVGRYILVPNAVLFSQVVINYTIVQESPYMLDEVVVRI
                     TYNSNWDRAEEILLKTAEEVTGDVIAATGVKPYIRSDLYDYGVNLRLRYQTRVKDRAE
                     TAYLLNKNMFKELQKCPDVDLAMPYVYSNRAGAKLVEITTEKGAVKEIPIDEITPSAR
                     ELDPHDVEQVAQSIALQGLLQPIVVTRNNATGRYDIQAGDLRLAACKQLGWKTITALV
                     QDAKEPKPVVPPISPLNPKA"
BASE COUNT         2271 a         3607 c         3590 g         2484 t
ORIGIN      
        1 atgcgttgct cctcctttca cctcaactcc accggcgggc acgctccgta cgcggcaaat
       61 ttactgagga acgaggaggg aggcaagggg aacaagaaaa gagcgtgaac tagggggctt
      121 tcacaggctc gagatcgcgg aaatgcatcc accagaccat gtcgagcgcc tcgatgcgcg
      181 tcagcccagg atccactgcg ctcagtgctt gccacgttcc ggcattttcc ggcgcgttca
      241 ggtccgcgac aagggcgtca agcgcgtggc gcactttggt cgcgtccttg acggtgtccc
      301 caacggtgcg ccgttctggc catcgatggt cgagatacga ttcgaatatc gctcggttgt
      361 cgagaacggg aatcaggtgc gggcgtttct tgtgcaggac cttcgtcgcc aacgaggtcg
      421 cgaagccggg ccatttgacg atctctgaga tcagttcgat gatccgctcg cgttcgtcag
      481 gcgttgtctc gtgcaacgcc ttttccggca acttcgacaa gtcaacctcg taggcgtgtg
      541 caacgaggga ggcgaaggca cgccagcctg cgttggaatt gaatgcaagg gttacggcaa
      601 ggtcctcggg taccagcctg tcccacggtg ttatcggttg gtatgccaga taagcgtagc
      661 cagcattctc acgaaaacaa cgcaatttgg ccgcgcaacc gttgattttt cgggcgaaag
      721 gggatagggt caacgaatcg ttcattttct tggcattccc gcttcacttt ctgaaaactg
      781 cctcggccaa gcacggcctt cgcacgcttc cgcttgacga tagccaaata ccgttgcggc
      841 gtcaaacgcg ccgctcccgt tcgctccgcc ccgcgacgcg cgcctctgtg gattcccgcc
      901 ttcgcgggaa tgacggcttc ggggagcggg tccgcgtttg gtgccggggc aagcacgcgg
      961 aatgacggaa ccctggtgcg ggtctccaag gggggggctg cggacgccgg gggtgtgcgc
     1021 agcgttgtcg cgccggggcc tcgcagatca gaggagctga caacccggcg gtctggctgg
     1081 ctcccgcatt tccctctata ttccctctcg ttctgttccg tcgccgaaag ggtgtgcaat
     1141 tatggataac tacgagtttt gcgtccagta ggttctcgac aagggacgta cggacccgat
     1201 ccgggttctc gattatggat gcggcgccgg ggagatcgtg aagctgctcc gcgcacgaaa
     1261 catcgaggca ttcgggtgcg acgtgtatta cgacgccgcg gattattcga acacgatgga
     1321 tccggcgctg ttcggcacct tgttgaggaa gatggaagat gggaggatac cttttgccga
     1381 ttcggcgttt gacttcgtca tcaacaatca ggtgatggag cacgtggaga atctggacgc
     1441 cgtccttgca gaaatatctc gtgtgctgaa accgggcggg tttgtgctca gcctgttccc
     1501 tgacaaacgc gtctggcgcg aggggcattc cggtatcccg tttttccact ggtttccgaa
     1561 aggcagtcgt ttccgggtgt actacgcatt ggtcctgcga acgcttggcc tcggctactt
     1621 caaagaggga aaaccgcgca aaaaatggag cgaggactac tgcatctggc ttgacaaatg
     1681 gacatattac cggagccgga aggagatccg cgagacgttc gcgaaacatc tcgcggaatt
     1741 gaaccacatc gaggtgtact ggttgcacaa gcggctgggg gcacggaggg taatcgccgc
     1801 atggctgcct gccgtaattc agcgctacgt cgtctggaag cttgcagggc tcgtgctgga
     1861 agcgcggaag gcattctgaa cctgctgcca acgaagaagg ggagcgcggc gaaccgtgct
     1921 ccccttggtc atttcttgtc gcaggtggct atcgaactgc cgccttgatc cacctgttga
     1981 ggaagtccac cgcggcggcc ttgttttcca gacgcaacgg tgggcgtggt acctgctcct
     2041 ttatttcgat cacgcttccc cttccgatcc aggaatcgag cgtacgcggc gtggtgcgga
     2101 tcgcttccgc gggcaactcg gtcatgatca gcagggggcg gtcgccgtat tcggccatga
     2161 tggccggggt ggtgagccgg ctggattccg gaggagagaa cagcgcgacc gcggcgactg
     2221 tcgggtcggc cacagcttca tgcagtccgc agtacccggc tatacctgct cccagaacca
     2281 ccaccttcgc gccgggggct tttgaccgca cgaacgccac ggcggctttg acgtctttca
     2341 tgtaatacgc aagcgcgtgc agggtgtcgt ttccgaccca gacaggggac gcaccaacgg
     2401 gcatctggct atcgccctga tcccgcaggt ccacggtaag caccgaccag cccgatgcag
     2461 caaactgctg cgccgtgctg gcccatgttc cgcgagagtg tgtcaacgcg ggcaggcaga
     2521 cgatgcttgc gcgcgagttc ggaacaccga accacgttcc cacgagtgtc acactatcct
     2581 cggtaattat ctccaccgtc tgcgcttcga cagtgacggg cacgcattcc ttttcctcac
     2641 cgccacaacc cacaaaaaac gctgtcgcta cgaccgcggc gcacagaacg attttccgta
     2701 ccatcgcatc cacctccccg cgtgaaccgc cgttgcatac cctgtcccaa ggcttctgca
     2761 aggacgctag taacataggg tttgaattgc gaaaaaccaa accttggtac acaaaacccg
     2821 tcgcgaactc tcctacgaag tggccggatt cggtttgtcc gggttcacaa ggaggtcgcg
     2881 catcgccttt ttcaggtcat ccggtgcgaa tttgccctgc cgggtgagct ccgtcagcgc
     2941 cgagagcgtg atatgttccg cgttgacctc aaagaaacgt ctcaacgcgc gccggccgtc
     3001 gctcctgccg aatccgtcgg tgcccaggca gaccggcggc gtggggaacc agcgatcgat
     3061 cgaggcaggc agcgctttca cgtaatccga agccatcacg aacgcgcctg tttcctgttt
     3121 cagcaccgac gaaacgtagg gttccttcgg cgggtcgaac ggatgaagga tgttccaccg
     3181 ctccgcaccc aggccgtcgc ggtagagctc cttgtagctc gtgatgctcc acacatcggt
     3241 aggcacgttg tacacgtcct ccagcaggtg ctgggctttc agagcttcgt tcagaatcgt
     3301 ccccgaaccg aacagattcg ccttggcttc tgaggccggc agcgacgccg ggcggaactt
     3361 gtacatgcct ttcagaatcg cagcgggatc cacatgcgca ggcatggcag gcatcgggta
     3421 gttctcgttt tccaccgtca cgtagtagaa caccgactcc tgctcgtgat acatgcggcg
     3481 cataccgtcg cggatgatga cggcgagttc aaacgcgaat gccgggtcgt acgcgatgag
     3541 attgggcacg ggatacgcga ggagatggct gttgccgtcc tgatgctgga gaccttcgcc
     3601 tgccagtgtt gttcgacctg cggttcctcc gatcaagaag ccgcgggcgc ggatgtctcc
     3661 cgccgcccag acgaggtccc ccacgcgttg caggccgaac atggagtaga aaatgaagaa
     3721 cgggatggtg ttcacgccgt gcgtggcgta cgcagtgcct gccgcgatga acgaggccat
     3781 cgaacccgcc tccgtgattc cctcctcgag aatctgtccg tccctggctt ctttgtagta
     3841 aagcaggttg tccctgtcca ccggatcgta caactgcccc acgtgggagt agatgcctga
     3901 ctggcggaac agcgcttcca ttccgaaggt gcgcgcttcg tcgggtacaa tcggcacgat
     3961 gagtcggccc cattccgggt cgcgaaggag ctttgtcagc agccgtacga acaccatcgt
     4021 tgtcgagaca cctcgatctt cggtgccttt ggcaaactca gcgaaaaggt cctcgtctgg
     4081 tggcggaagc ggggcattcc gtggcttgcg tgagggcgcg aatcccccga gagctctgcg
     4141 gcgctcgagc atgtattcga tctcccggct gccgtccggg ggccggtaga atggcgcggt
     4201 cgccaggtcc tcgtccgaaa tcggaattcc gaaacggctg cggaactcag acagctcctt
     4261 ttcgttcatc tttttctgct ggtgcgctac gtttttccct tcaccggctt cgccgaggcc
     4321 gtaccccttg atcgtacggg ccagaacaac cgtcgggtgg cctttgtgct cgaccgcagc
     4381 cttgaatgcc gcgtagacct tctcaggatc atggcccccg aggcgcatct tctgcaggtg
     4441 ctcgtcggag tagttctcca ccatcttcag gaggcgggga tccgcgccga aaaagtgctt
     4501 gcggatgtac gcgccacctt cgacgctgta tttctggtac tcgccgtcca ccacctcgcc
     4561 catgcggcga acgagaattc cgtcgcgatc cgccgcgatg agttcgtccc actcgctgcc
     4621 ccagaccact ttgattacgt tccagcccgc gccccggaag gcggcttcca gttcctcgat
     4681 aatgtggccg tttccgcgca cagggccgtc aagccgctgg aggttgcagt tgatcaccca
     4741 gatgagattg tcgagttttt cccgcgccgc gagcgtgatg gcaccgagtg attccggttc
     4801 atccgtttca ccgtctccca gaaatgccca caccttgccg cctgtgtcct ttttcaggcc
     4861 acggtcttcc agatagcggt tgaatcgcgc ctgatagata gacatgatgg ggccgagacc
     4921 cattgacacg gtggggaatt cccagaactc aggcatcagc cacgggtggg gatacgaaga
     4981 cagaccgccg cccgggcgta actcctgacg gaagctttcc aggtcctctt cggtcaaccg
     5041 tccctcgaga aaggcgcgcg cgtagatacc gggcgaggcg tgcccctgga agtagatctg
     5101 atcgccgtcg tagccgttac cccgtccccg gaagaagtgg ttgaacccga cctcgtacag
     5161 cgtggccgcg gatgcgtacg tggagatgtg gccgccgatg ctgctgctcg cgcggtttgc
     5221 ccgaaccacc atcgccatgg cgttccagcg caccaggctc ttgatcctgc gctcaatttc
     5281 ccggcttccc gggtacggcg gctgcttttc cggcggaatc gtgttgatgt gcggtgtgga
     5341 cgcggaaaat ggcacgtcga cgcctgcgac gcgagcctca ttctgcagca tctgaagcag
     5401 gccttcaacg cgttcaggtc ctccgctgcg caatacgtaa tacagagaat ccagccattc
     5461 ccttttctcc gcgaggttca tcccagcggg ttctccggac cgggtcgttt cactcatcgt
     5521 tggcgttcct cagaggggaa aaagcgtcaa atccttctgc gaagggccgg caagatagaa
     5581 gcgtcgaact aatgtacgcc cctcgccaca cgaaggcaaa atcgatatga gaacgctttt
     5641 tcgcagattg aacgttccca ttgaagccat cgcggtgtac ttttgtctca tgggtcgcgc
     5701 attctggaac cggcgatggc ctgccgcgtt ccctgcagga cgcggcttcg gcacggttga
     5761 tctccccgga gcgaactttg ttaacactga accggctggt cttcttcctc acgttcgttt
     5821 ccgcgtcggt ctcctcggga gccacgcggt acgcagggga cttccttgcc gtgggggtca
     5881 gcccgcgcgc taccggaatg ggctccgcgg tgacggcgat agtgggcggc ccggaggcgt
     5941 cgtactggaa tccggcgggc ctcgcggcgg ggatgaccgg cgcacccgta tccgtcgcga
     6001 tggaacacgc ggaacggttc ggcggggttg tgaagcacga cgccgtgagt gctgcaattc
     6061 cgctttcgcg aagcgttctc ggggtgtttc ttttccgggg cgcggtggat ggaatcatct
     6121 tcgccgactc caccacgctg gtagacccaa gcctgccgtt gagcgaatcc aatatgccgg
     6181 atccgtcgaa aacgcggacg ttctccaacg ccgactacgt gatccacgtg gcaaccgggc
     6241 acacggtgct ccgcacgctc agggtgggag cgggagtgaa actgatccgc cggacgatcg
     6301 ggggaacggc cgcgttcggg tacggcgtag acctcggtgc tcagtgggag ccgcgcccgc
     6361 agatcgcggt gggaatactt gtgcgagacg ccacgaccac gcgcgtaaac tgggataaca
     6421 gccgcaccga tgttgtctac cccacggtgc ataccggcgc ggcattcggt ttcccgttcc
     6481 ctcgttttgg tgggcggctg gtggtcgccg gaaactcaac cgtcggatcg gaatcggggg
     6541 ggtacggagg gttcgctccc tggcgcgtgc tgaatgccag gaacgtggcg aacctcggag
     6601 ccgaatacga gtggcgggag acgattgcgc tgcggcttgg aagccagaac gtgcgcggga
     6661 tgctgggacc cggctcggcg gaagtaaccg ccggtatggg attgcgcggt cacctcccct
     6721 ggttctccgg tgtccggcgc gtgggagtcg atctggcgtg gatccgccac accctccgcg
     6781 attcgttccg tctcggcgca acggtggaat ggtagcaaac aggaaggggc tcacattcgt
     6841 gaaacacgtc gtttcgctcg tatcaacagc ggggttgtgt ggcgttgtgg cgtgcagctc
     6901 gatttcgtca cccacggtgt atgtgcgcga cccatcgtac gatttccgcg gaaaaaccgt
     6961 aggggtgtgc gtgattctcg acccggtgga gcaggaggcc ctcagcgacc agcgggtggt
     7021 caaccgggca acctcgtcgg ggcagaccga agggtatgcg agcgaaatga cggaacagcc
     7081 cctggaggaa gacaccgggc ttctgacgcc gccgaagacg gtcacgagcc tgcagacgaa
     7141 gttgaaacac tggcggtact acgacgaaga tttcaccctg gcgatggttg atgaggtcag
     7201 cgctgaactt aaaaaacgag ggttcaaggt tcgggtaatt ccggtccccg agggtcaggt
     7261 tgaactcgtt gaactcgccc aacgcgcgca gtccgatacg tgccagatac ttgcagtggt
     7321 aacggttact gtcgcgcgga gctggaatat caggcatcag cagacactga gaagcacgca
     7381 ggatctcagc tcttcgatga ggtgggtttc ttacaccggt ggacttgttc tctgcaacat
     7441 tgccatgttc gacatcgcca cgaacaaagt cgtgtggcag cattctcagc gagttgtgcc
     7501 gctctcccgc acagttccgg taatcgccga gctttacgac aacgaagtgg cttccacgcg
     7561 cttcagctcg cggcccggtc tgtatcaggg gtggatgtac cgccaggcga tgccgcgagc
     7621 cgtcaggctg atgttcgaaa cagcggaatc cggcttcatt ccgttgccgc gtggttccga
     7681 atcggtcatt gcagacaccg ccccggctcc attcgcctcc ggctgccggg tgttcgtcag
     7741 gcctgattcc tccagccgcc tgtggttccc ggcgaccgta attcgcgact cggccggcgt
     7801 ggtttccgtg caatgggcag acgggatatg ggaggcgtac ggccgacgag tcgaataccc
     7861 gcggtcagac gtgcgcccac gggatgagtt cccggagatc gtctgggtgc gaacgcgcga
     7921 cagactggaa ttcaccccgt accggttcgt ccgcgtggac gtgcgcggac tcgtgcacgt
     7981 cacccgggcg ggcgatatgt cgcccagcac cttcctgccg ggccggatcg gcgtcgtgct
     8041 ggagtaagag caccgtcccc caaaaaaggg aaaggctgcg cgagagcacc gcgcagcctt
     8101 gtttctgtct gaacgcgtgt cgaagcgatc agtccttctc ttcagcttcc ttctgcttct
     8161 ggaattcttc ctggattttc accatgatat ccggcggaac ttcctcgtag tgatcgaact
     8221 cacgggtgtg aagtccgcgt ccgccggtca tggaccgcag gtcggtgctg tagcggaaaa
     8281 gattcgcgag aggaaccagc gcccgaatga cctggaaggg ccccttcgca tccatcccga
     8341 gaatcttccc gcgcctgcca gaaagatcgc ccatcacatc gcccatgaac tcctcgggga
     8401 cagtcacctc aattttgtag atgggttcaa ggagaaccgg acccgcttcc cgcatggcgt
     8461 tcttgaatgc gttcagggcg gcaagtttga aggcgatttc ggaactatcc accggatggt
     8521 aggaaccaaa aaacaccgtt gacctcacgt ccacgacctt gcaccccgcg atgatccccg
     8581 attccatcgc ctctaggacg cccttttcca ctgcagggat gaactttccg ggaatgactc
     8641 caccgacgat ggcgtccacg aactcgtatc ccgcgcctcg ttccagcggt tctatccgaa
     8701 gatgaacgtc tccgaactgc ccgcgcccac cggtctgctt cttgtgacgg tactgcgctt
     8761 cggccttttt acgaatggtt tcgcggtacg caattttcgg tttctcgagg tctacgacga
     8821 ctccgaagcg ctctgcgagg cgcttcacca tcacatcgag gtgaagctcg ccctgtccgt
     8881 agatgagggt ctggttcaac tcaccatcga cctcgtggta gaaggtcggg tcttctgcgt
     8941 ggagccggtg caaaccgttt gccaccttgt cttcatcgcc cttcgtgcgg ggaacaacgg
     9001 cgaaactgat gttcggtttg ggccattcca ctcccggaag aagcacctgg cggctcttgt
     9061 cgcacagcgt gtccccggta tgcgtggtcc gcagcttcac aagcgcgcca atgtctcccg
     9121 cgttgatgcg gccggcgtcc gtccggtcct tccccagcat gaagaagctc tggccgattc
     9181 gctccgaagc gttctgcgat acgttcgcca cctcgatatt ctgcgtgatc ttgccgccgt
     9241 aaacccgcac gaaggaaagc tcccccacgt gtgcttcgga cacggttttg aaggtgtacg
     9301 cgcacagagg atcggcctct gagcattgga tctgcacttt gccgtccgag cctgccaccg
     9361 tggcttccac aggccccata tctgcgggcg agggaagata ttcggcaatc cattcgagga
     9421 ttcggtcggt gccaacgttg tgatgcgcat cggacgcaag cacgggatac accatgcggt
     9481 tcttgatcgc cgctttcaat ccggcgatga agccatcttc ctccagctcg cctgcttcga
     9541 ggtatgtctc gaggagcgct tcgtccgatt ccgccaccgc ttccatgagc gcttcccgca
     9601 tttgttgcgc ccgggaagca agttcgccgg aaagctcgct gattttcgcc acacccttgc
     9661 caccgggttc gtacgtatac agcttcccgg tcaccacatc cacaatctgg ttgaaatgct
     9721 cgccctggtt gacggggaac tggcacacaa caacgctgct gccgaatcgg tccttcgcct
     9781 gatcgaccgc ctgctggaaa tcagcatgtt cgcggctgca cgcgttgatc acgatgagtg
     9841 ccggtttgtg gaattcggcg gcataccccc agatgagttc cgtgccgact tcgacgcccg
     9901 tctgcgcatt gatgaacacc gcgacgttgt ccaccgcgcg caacgctcca accgcttccc
     9961 cgatgaaatc ggtgtatccg ggcgtgtcaa tcacgttcat cttgatcgcc ccggcctcga
    10021 actgcaacac cgttgcgttc agcgaaaact tcctgagctg ctcatcggcg ttgtagtcgc
    10081 ttaccgtgtt cccgtcggtt accgacccca tgcgggttgt caccccgagg gtatacagca
    10141 ccgcctccac gaaggtggtt ttcccggctc ctccgtggcc gacaaacgcg atgttccgta
    10201 tggtgtttgc gctgtgttcg ttcatgcgaa tgctcctgca gatttacggg aagatgaggc
    10261 gatgcaccgg taggcggttc gcaaacatga accaataaaa atacgagttt gccgtacaag
    10321 gtcaaggaag ggactggcga aaggcgattc catggggccg gccacggaaa accggaagcg
    10381 gacgatgcgg gcggatgctt cggttgccgc attactccgc cgatcccgtg caggagagcc
    10441 cggaacgcgg acaccgacag gacaaaatgc ggaggatagt tccccggggc acactcctcc
    10501 tctccgtcct tatttgcgcc tttcttggta tattacatga atctgaaacc agaccagaaa
    10561 acccgtggcg gaggtaagcc ggcgtgaagt tcaccagtta ccagaaaaag acactcaagg
    10621 cgcttgtgct gtggagtgtc atcgcagccg ccgcgtacgc catcgcaccc agggtgcctc
    10681 gattgttgcg attggcgtcc ggcatgaatc tgctggagtt gatcggcctc ctcgcgttga
    10741 ttcttgcgat cggcgaggcc cttggcttgc tcactcaggt cattctcgta ggaaaacgga
    10801 agccgccggt cgagggcgcg atggtgggcc gcatctaccg gcttgtggcg gtgctggcag
    10861 tgcttctcgg catcgcatac ggattcggca agctgggcac catcggtgcg ttcttcgcca
    10921 tgttcggagg tatgctcctc ggctggtcct tgcaggcacc ggtcagcggt atcgcggcat
    10981 gggtgctggt atcgctgaaa cgcccgttca ggcccggtga ccggatccag ttccccaatc
    11041 ttggacttgt gggagacgtt cacgataccg gcatcatgta cacgatgctg aaccaggtcg
    11101 gcggaacggt cggcagcgaa gaggcagtcg ggcggtacat ccttgttccc aatgccgttc
    11161 tgttcagcca ggtagtcatc aactacacca tcgtccagga aagcccctac atgctggacg
    11221 aagtggtcgt ccggatcacg tacaactcca actgggaccg ggcggaggag attctgctca
    11281 agaccgcgga agaagtcacg ggggacgtga tcgctgccac gggtgtgaaa ccttacatcc
    11341 gctccgacct gtacgattac ggtgtcaacc tgaggctgcg gtatcagacc cgtgtgaaag
    11401 atcgtgccga aaccgcgtat ctgctgaaca aaaacatgtt caaggaactc cagaagtgcc
    11461 ctgacgtgga cctcgccatg ccgtacgtct actccaaccg cgcaggcgcc aagctcgtcg
    11521 aaatcacaac ggaaaagggt gccgtgaagg aaatcccgat cgacgagatc accccgtcag
    11581 ctcgtgagct ggacccgcac gacgttgaac aggtcgcgca gagcatcgcg cttcagggtc
    11641 tcctgcaacc gatcgtcgtt acgcgaaaca atgcgacggg aaggtacgat atacaggccg
    11701 gcgatctccg cctcgccgcg tgcaaacagc ttggctggaa aacgatcacc gctctggtgc
    11761 aggacgccaa ggaaccgaaa ccggtcgtcc cgccgatttc tccactcaac ccgaaggcat
    11821 gatgaatcat ccgaggggtg gcgcgcgcca ccccttcccc ggttttcgca tcccgacagg
    11881 tacttcttca tgattcctca tcgaacactg gcggcactcg ctctcgccgc cttctttctc
    11941 ggaggctgtt cg
//
LOCUS       DAOSOO010000085        11863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5352_length_11863_cov_6.626948, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000085 DAOSOO010000000
VERSION     DAOSOO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_11863_cov_6.626948"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLT86_07615"
     CDS             <1..209
                     /locus_tag="PLT86_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36516.1"
                     /translation="ESPNRVVIARRSGAAAKPVVAQMAAFLDCIEQRKKPEISARQSV
                     RSLEVIWRLYEAEETGVVADLRGV"
     gene            complement(370..1731)
                     /locus_tag="PLT86_07620"
     CDS             complement(370..1731)
                     /locus_tag="PLT86_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT36517.1"
                     /translation="MSEDEELIETDLFDGVEKPDTTAPLADRIRPRTLAEFAGQRHII
                     GEGALLRRMVEQDRLVSLLFWGPPGTGKTTLAFVLARMLNARFVTMSAVTSGVKDVKA
                     VIERAAMEKRRGRRTILFIDEIHRFNKAQQDALLPHVERGTVIFIGATTENPSFDVNS
                     ALLSRCRVFVLKQLLPEDIEQIVRRALADRDRGLGGLELVMDDDAVDALAKLADGDAR
                     IALNALEAAANVVSAGDRHITKQVIIEALQQRTYDYDRAGEGHYNLISALHKSVRSSD
                     VNAALYWLARMLDAGEDPRYLARRMVRMAVEDIGLTDPRALSVALAAHETYHMLGSPE
                     GELALAEAVIYLATAPKSNATYVAFGKAMADAREYGSLPVPLHIRNAPTKLMKDLGYG
                     DGYQYDHDLPDRFSGQQCLPDELAGREYYEPTEQGYERTVAERMRWWAELREKLKRDK
                     GGE"
     gene            complement(1963..2157)
                     /locus_tag="PLT86_07625"
     CDS             complement(1963..2157)
                     /locus_tag="PLT86_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HOT36518.1"
                     /translation="MVSEGLTRESEITCPHRGFKKVETMPIDACQHFYKCEGCGVVLR
                     PKSGDCCVFCSYGSHPCPPV"
     gene            complement(2239..2994)
                     /locus_tag="PLT86_07630"
     CDS             complement(2239..2994)
                     /locus_tag="PLT86_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36519.1"
                     /translation="MNYQRHAWGWALAAACAVGLLNAPAHAIAGFGIHAGKDFVSISE
                     GSFDRAKFEAAGTRLGLTGVNWANWNSVTLTRKEISSPWLIGVHAYIDAIPYIDLEVS
                     VDGAISQYRVDYRSTLNPAVNESKDVYFGRVGAYGTVRRDIVKFPPVVPIAALYFGGG
                     LSYNFIAPVAGPDLIVNAYGTDNPTTRQPDIAKLVEREGTFGWHGLVGFRIKPPVVPI
                     ALRLEGKYTSTGISTYERPKSIFSAYLGTSFAF"
     gene            complement(3208..3585)
                     /locus_tag="PLT86_07635"
     CDS             complement(3208..3585)
                     /locus_tag="PLT86_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT36520.1"
                     /translation="MAKFQAVTSATINTIIGENSRVVGDFDIAGSIKVEGFLKGKLTT
                     SERLVIGLGGVVLADANVRDAIIGGTFTGTLYAETRVEMESTAKVRGDVRTKQLVIHE
                     GAEYEGNISRGEEPSAEAEGDTG"
     gene            complement(3620..4459)
                     /locus_tag="PLT86_07640"
     CDS             complement(3620..4459)
                     /locus_tag="PLT86_07640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT36521.1"
                     /translation="MGIRTAIQGLRSAMRVIAHAGRAVWLFFSERRPAPKPDHPLVVK
                     VVYPGGAATRDFSFPLRTVWKAKIAVAVVFVLFFVGLAAIGDAAYSRWRLYHLNAQLR
                     EAQTNEQLTLEMRRQLAEIWLINERLQRMLGRHESYRTRQTVVYRLPQGLPVSRWVGV
                     AFGVPNALPEDMRACYLCAPGSLVLATADGKVTDLSWDPRNGDVVTIDYGEGVTARYG
                     RDALFFVEKDELVRKAQPIGVIHAPDSLSTPLLFYQVTAEGEEVETVPYMVVGGTTGI
                     YTE"
     gene            4573..5775
                     /locus_tag="PLT86_07645"
     CDS             4573..5775
                     /locus_tag="PLT86_07645"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT36522.1"
                     /translation="MVFFLTAIPVAVPVYSLPSAGRFRFPGACRNFPSGEVTLPQPRI
                     VALRRAMRAERVDRFLVTCPEHVRWLSGFSGSSAWLVVGVREKVLLTDFRYRDQAAAQ
                     APDWRAVITQNGLVACVRELAAGWGDSRVGFESAHLTVKDHRALTSPFKDGDTPLSVQ
                     WVATEQIIERELFVKDEAEIAAIARSAAIADAAFAEVLPLVKPGVTERDLATELEYRV
                     RKLGADRMAFPPIVASGPNAALPHAQPSDRRIRNGDMVTFDIGAQRDGYASDMTRTVA
                     VGKASKKLQKVYGIVLEAQMRAVAAVRPGLSCVELDSVARSYIQEQGFGEAFGHSLGH
                     GVGLRVHDGPSISWRSQETLAEGMVITIEPGIYLTGWGGVRIEDLAVVTDKGCRVLCS
                     TPKELRIV"
     gene            5830..6387
                     /gene="efp"
                     /locus_tag="PLT86_07650"
     CDS             5830..6387
                     /gene="efp"
                     /locus_tag="PLT86_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT36523.1"
                     /translation="MATTQDFRNGFAFRMDGNIYFIVEFQHVKPGKGGAFVRTRLKNV
                     QTGAVIDRTFKSGEAVEEIRVENRDMQYIYKEGDHYVFMDTETYEQIHLPHEIVDDIA
                     DLLKENEVVTVIIGDDMPLLVELPNHVTLTIADTDPGVRGDTATGGTKPAKLETGAVV
                     QVPLFLGVGEKIRIDTRTRQYLSRA"
     assembly_gap    6403..6502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6503..6865
                     /gene="accB"
                     /locus_tag="PLT86_07655"
     CDS             <6503..6865
                     /gene="accB"
                     /locus_tag="PLT86_07655"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOT36524.1"
                     /translation="SQAFPHIVAPAPVVQIPDSPPAAPAKETQAAPAAAKAPDANLLE
                     IKAPMVGTFYRAPAPEAAPYIETGAHVAKGQIIGIIEAMKLMNEIPADVAGTVVEIAV
                     ENAEPVEFGQVLLRVKPD"
     gene            6871..8223
                     /gene="accC"
                     /locus_tag="PLT86_07660"
     CDS             6871..8223
                     /gene="accC"
                     /locus_tag="PLT86_07660"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOT36525.1"
                     /translation="MFSKILVANRGEVALRIVRACRELNIATVAVYSEADASSLHVRF
                     ADEAVCIGPPDARQSYLDPARIIAAAEVTGAEAIHPGYGFLAENARFADICAECGFVF
                     IGPTGDMIRMMGDKAVAKKTMADAGVPVVPGSDGPLKSAEQALALAKDHGYPVMLKAV
                     AGGGGKGIRIVTSPEGLVRAYDLARNEAETAFKLGDLYLEKFVDHARHVEIQILGDSE
                     GNVVHLGERDCTVQRRRQKLIEESPSPAVTRDLRDRMASAAVAGAASVGYRNAGTIEF
                     LLAPDQQFYFMEMNTRVQVEHPVTEMVTGTDIVKEQIRIAAGLPLSFRQEDIILQGHA
                     IECRINAEDVAHDFRPTPGRITEWHVPGGIGVRIDTHAYAHYAVPPYYDSMLAKLIVH
                     APTRAEAIVRMKRALGEFFVEGVPTTIPFHSRVMSDSVFQNGHYDMGYVEDFFTRTEI
                     "
     gene            8236..8619
                     /gene="gcvH"
                     /locus_tag="PLT86_07665"
     CDS             8236..8619
                     /gene="gcvH"
                     /locus_tag="PLT86_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT36526.1"
                     /translation="MDFPDDLAYTRDHEWLRLEGETGTAGITDYAQRELTDIVFVEFV
                     APGTVVRQGERFGTVEAIKAVSDLFSPVDGEIVEVNTGLSTHPEWINQDPYGKGWMVK
                     IRVSDPAQVRTLLSAADYRTLVASG"
     gene            8635..9978
                     /gene="gcvPA"
                     /locus_tag="PLT86_07670"
     CDS             8635..9978
                     /gene="gcvPA"
                     /locus_tag="PLT86_07670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT36527.1"
                     /translation="MADPHPFIPCTDSDLAEMLAAIGVRSFEDLVAPVPDAIRAASAL
                     NLPPRLSEHEALSLLRRRARENAPDAVCFLGAGMYDRIIPAAIDAVVQRSEFTTAYTP
                     YQPEVSQGTLQAIYEFQTMVCELFGMDVANASVYDGASALTEAILLSHAHTGRRKVVF
                     AGPVHPRYRSAFSSITVGIGLESVEAPFSDGGADAAAVAERVSGETACVVVQQPNFYG
                     VIEDIAALAAIAHREGALLVVSADPVALSLLEAPGKQGADVVVGEGQPLGIPMSFGGP
                     ALGLFAVRDELKRRLPGRLVGRTVDTRGQQAFVLTLQTREQHIRREKATSNICSNQNL
                     MAIAATMYLTLMGPAGLRTVAENSLQNAHYAADQIEARTVFRQVHPRPFFDEFVVKCP
                     VPAREVVDRLVPRGILAGVDMAQFGGDPAHLMIAVTEKRTKEEIDALVAALGEIG"
     assembly_gap    10111..10210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10515..>11863
                     /locus_tag="PLT86_07675"
     CDS             10515..>11863
                     /locus_tag="PLT86_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36528.1"
                     /translation="MNFRPRNPNRHLWDTWFYDHNGEYHLFYLSNRIADDGRWIPWDA
                     FSLATSRDLLHWEEKGEVFHKSENPADWDSELITTGDIIRWRGRWYMTYRATQDRVEK
                     NNIIVSDDLLNWQRISPSPVLVTSEPYEHDPAQTINNYVEMRDVTITELPDGTLEGVF
                     TARLNSGPHTGRGVLARAFSADMLHWRMGEPLFAPGRFNVIEVPSRFRHGGRHYLLFC
                     VNSHLESALENRNYPSMTWCTGYAVSDRCDGGFDYVPEQVLGIGDAYVGRLVNAGGET
                     LFFQHLCGRRPAFALPKSVSFGADGTIRLGYWRGVDSLIRGEGIRGFSTLQFFEDGGK
                     TVCGGNIAPETCTLENAAGFGDAYLPGAFSDFVLDVDIQFDRCVSAGVLLNADVPSRG
                     AVMATLRSDRGAFQVGRSRKVYNHLAPNGVRHEYLSDALKRGTVHLKVVSRSEGFDW"
BASE COUNT         2144 a         3687 c         3689 g         2143 t
ORIGIN      
        1 tggagagccc gaaccgggtg gtgatcgcgc ggcggtcggg tgcggcggcg aaaccggtgg
       61 tggctcagat ggcggcgttt ctcgattgca tcgaacagcg gaagaagcct gagatcagcg
      121 cccggcagtc ggtgcgcagc cttgaggtga tctggcgtct ctacgaggcg gaggagaccg
      181 gcgtagtggc ggatttgcgg ggggtgtgag aaaggcgcgt ccctccaaac tcacgcggac
      241 catcaaaccc tgctgccgcc ggtattcgtc caaagggacc tatacgacag atacacttgc
      301 gatgcgcgct cgcctgagcc gtctatcggc ctttggtgtg tttcgtgtcg ttcgtggtga
      361 gaacccgttt cactccccgc ctttgtccct cttcagcttc tcacgcagtt ccgcccacca
      421 gcgcatgcgt tcagcaacag ttctctcgta gccctgctcc gtcggttcgt agtattcccg
      481 ccccgccagt tcgtccggca ggcactgctg gccgctgaac cggtccggca ggtcgtggtc
      541 gtactggtag ccgtcgccgt agccgaggtc cttcatgagt tttgtcgggg cgttccggat
      601 gtggagcggt accggcagac tgccgtactc gcgggcatcg gccatggcct tgccgaacgc
      661 gacgtaggtg gcgttggatt tgggcgcggt cgcgaggtag atcaccgcct ccgcgagggc
      721 gagttcccct tccggtgaac cgagcatgtg gtaggtctcg tgcgcggcga gggcgacgct
      781 cagggcgcgc gggtccgtga ggccgatgtc ctccaccgcc atgcggacca tccgccgggc
      841 gaggtagcgc gggtcctccc ccgcgtcaag catgcgggcg agccagtaga gtgcggcgtt
      901 gacgtcggaa ctgcgcacgc ttttgtgcag ggcgctgatg aggttgtagt gcccttctcc
      961 cgcccggtcg tagtcgtacg tccgctgctg gagcgcctcg atgatgacct gcttcgtgat
     1021 gtgccggtcc cccgcagaaa ccacgttcgc cgccgcttcg agggcgttga gagcaatccg
     1081 cgcgtcgccg tcggccagtt tcgccagcgc atccaccgcg tcgtcgtcca tcacgagctc
     1141 aaggccgccc agcccccggt ctcgatcagc gagcgcgcga cgaacgatct gttcgatgtc
     1201 ttccggcaga agctgcttca gaacaaacac gcggcagcgg gagaggagag ccgagttcac
     1261 gtcgaacgac gggttttccg tggtagcgcc gatgaagatc accgtgccgc gttcgacatg
     1321 cgggaggagg gcgtcctgct gggctttgtt gaagcggtgg atctcgtcaa tgaagaggat
     1381 cgtccgccgc ccgcgtcgtt tttccatcgc ggcgcgctcg atgacggctt tcacatcttt
     1441 cactccgctc gttacggcgc tcatcgtaac gaaccgggcg ttgagcatgc gcgccagcac
     1501 gaacgcgaga gtggtcttgc cggtgccggg cgggccccag aagaggagag atacgaggcg
     1561 gtcctgctcc accatgcggc gcagaagcgc gccctccccg atgatatgcc gctggccggc
     1621 gaactctgcc agcgtgcgcg ggcggattct gtcggcgagc ggcgcggtcg tgtcgggctt
     1681 ctccactccg tcgaacagat ccgtttctat gagttcctcg tcctcactca tggccgactc
     1741 ctgaccgcaa gggaaaacgg acggcaccga ccgcgatacc gcccgtccgc aggaacctca
     1801 acgctcgcca aggggcgaac taccccctca agcagccgtc gaatgcgccc ttcgggcgtt
     1861 cgcaggcgcg gattccgctg gaagtatact tcccttccac tcggaaggca atcgggcctg
     1921 tcggcacggt tcacagggtc atgcgttgct cgccctttgc tgctacacag gtggacacgg
     1981 gtgtgagccg tacgaacaga agacgcagca atccccggat ttcgggcgaa gcaccacacc
     2041 gcacccctcg cacttgtaga aatgctggca ggcgtcgatc ggcatggttt ccaccttctt
     2101 gaaaccgcga tgcgggcacg taatctcgga ctctctcgtc aggccttcgc tcacgatcaa
     2161 cggccctccc gcacgcctgt aacaaaaacg ggcggtaccg atcgcgatac cgcccgtcca
     2221 gaggaatctc agcaaacgct agaaggcgaa cgacgtgccg agatacgcgc tgaatatgct
     2281 tttgggccgc tcatacgtgc tgattccggt ggatgtgtat ttcccttcca gccgcaacgc
     2341 gatgggaacc accggcggct tgatgcggaa cccgacaagc ccgtgccagc cgaaggttcc
     2401 ctcgcgctcg accagctttg cgatgtcggg ttgccgtgtg gtggggttgt cggtgccgta
     2461 cgcgttcacg atcaggtccg gtccggccac cggagcgatg aagttgtacg acagtccgcc
     2521 accgaagtaa agcgcggcaa tggggaccac cggcggaaac ttgacgatat cacgccggac
     2581 cgtgccgtac gcgccgacac ggccaaaata tacgtccttg ctctcattca ccgccgggtt
     2641 caacgtggag cgataatcca cccggtactg cgaaatggca ccatcaacag atacctcaag
     2701 atcgatgtag gggatcgcgt cgatgtacgc gtgcacgcca atcaaccacg ggctggagat
     2761 ctccttgcgg gtgagggtga cggagttcca gttggcccag ttcaccccgg tcaggccgag
     2821 gcgtgttccc gcagcctcaa actttgcccg atcgaagctc ccttccgaaa tggacacgaa
     2881 gtctttgccg gcatgaatac cgaaaccggc gattgcgtgt gcgggcgcgt tcaagaggcc
     2941 caccgcgcac gcggcggcaa gcgcccagcc ccatgcatga cgctgatagt tcattctggt
     3001 ctccttcttt caatcaggtt tccctgtgtg tgcgtatcct cgacaacggc cggacgcaac
     3061 agaaggttcc cggatgccgg cgtatccggg cggtaacagt gtctgtcggg aaaagataca
     3121 cgttttgcac ggttgtacgt aggatggcgc cttgttggca ttccagaact tcctgccccc
     3181 atcgcggcca cgggtgctgg cgaccaccta ccccgtatcc ccttcggcct ccgcggatgg
     3241 ttcctccccg cgcgagatgt tcccctcgta ttccgcccct tcgtggatga ccagttgttt
     3301 ggttctgaca tccccccgca ccttggcagt gctctccatc tccacgcggg tttccgcgta
     3361 cagcgtgccg gtgaaagtgc cgccgatgat ggcgtcgcgc acatttgcat cggccagcac
     3421 aacgccgccc aggccgatta ccagccgttc cgacgtggtg agttttcctt tcagaaatcc
     3481 ctcaaccttg atgctgccgg caatgtcaaa atcacccaca acgcgggagt tttccccgat
     3541 aatcgtgttg atcgtcgcgc tcgtcacagc ctgaaacttt gccatctgat gtgcctttcc
     3601 gaaatcgtga ccgccccggc tattccgtgt agataccggt tgtgcctccc accaccatgt
     3661 aaggcacggt ttctacctct tcaccttcag cagttacctg atagaacagc aagggagtgc
     3721 tcagggagtc gggggcgtgg attacgccta tgggctgcgc tttccgcaca agctcgtcct
     3781 tttccacgaa gaacagagcg tccctgccgt agcgcgcagt aacgccctcg ccgtagtcga
     3841 tggtgacgac gtccccgttt ctcgggtccc acgagaggtc ggtcaccttt ccgtcggcgg
     3901 ttgccagcac gagtgagccg ggcgcgcaca ggtaacaggc gcgcatatcc tccggcagcg
     3961 cgtttggcac accgaacgca acacccaccc accgcgagac agggaggccc tggggcaggc
     4021 ggtacaccac cgtctgacgc gtgcgatagc tctcgtggcg cccgagcatg cgctgcagac
     4081 gctcgttaat cagccaaatc tccgcgagtt gccgccgcat ttccagtgtc agttgctcgt
     4141 ttgtctgtgc ctcccgcagt tgcgcgttga ggtggtacag cctccaccgc gagtacgccg
     4201 cgtcaccaat cgccgcgaga ccgacgaaga acagcacaaa cacaaccgct acggcaattt
     4261 tcgccttcca gaccgtccgc aggggaaacg agaaatccct cgtggcggcg ccaccggggt
     4321 acaccacctt caccacaagg gggtggtcgg gtttcggagc gggtctccgt tcggaaaaaa
     4381 acagccagac cgcccgaccc gcgtgcgcaa taacccgcat tgcggaacgc aacccttgaa
     4441 tggccgtgcg gatacccatt cgtccctctc ttttcgcgac tgtatctgga acttggaata
     4501 tagcccgcgg tgttccaaac ggtcaacgtg acgcaggaca acaagcctga gaattgaccc
     4561 aaaccgctgg aaatggtatt ctttttgact gccattcctg tggcagttcc ggtctattca
     4621 ttgccatccg ccggccggtt caggttcccg ggtgcgtgcc gtaactttcc ctctggcgag
     4681 gtaacgttgc ctcaaccccg aattgtggcc cttcgtcgcg cgatgcgcgc ggaacgcgtg
     4741 gacagattcc tcgtgacgtg cccggagcat gtccgctggc tgtccggttt ctccgggtcg
     4801 agcgcgtggc ttgtggtagg ggtgcgcgaa aaggtgctcc tgacggactt ccgttaccgc
     4861 gatcaggcgg cggcacaggc gccggactgg cgcgccgtta tcacgcagaa cggtctggtt
     4921 gcctgcgtgc gcgagcttgc ggcggggtgg ggcgactccc gtgtcggctt cgaaagcgcc
     4981 cacctgacgg taaaagacca ccgcgcgctc acaagcccgt tcaaggatgg cgacacgccg
     5041 ctgagtgtgc agtgggtcgc aacagagcag attatcgagc gagagctttt cgttaaagac
     5101 gaagccgaaa ttgccgccat tgcccgctcc gccgccattg ccgacgccgc attcgcggag
     5161 gtgctgccgc tggtgaagcc cggcgtgacg gaacgcgacc tcgccacgga gctggaatac
     5221 cgcgtgagaa aactgggcgc cgaccgcatg gcgttcccgc caattgtcgc aagcgggccg
     5281 aacgccgcgc ttccccacgc gcagccttcc gaccggcgca tacggaatgg ggacatggtc
     5341 acgtttgata tcggtgcgca gcgggatggg tacgcgagcg acatgacccg gacggttgcc
     5401 gtggggaagg cctcaaagaa actgcagaag gtatacggca tcgtcctgga agctcagatg
     5461 cgggcagttg cggccgtcag gcccgggctt tcgtgcgtgg agctggacag cgtggcccgg
     5521 tcctatattc aggagcaggg gttcggcgaa gccttcgggc attccctcgg ccacggtgtg
     5581 ggcctgcggg tgcacgatgg gccgtccatt tcgtggcgca gccaggagac actggccgag
     5641 gggatggtga tcacgattga gccgggcatc tacctgaccg gctggggcgg tgttcgcatc
     5701 gaggatctgg ccgtggttac cgacaagggc tgccgcgtgc tctgctcgac gcccaaagaa
     5761 ttgcgtattg tttgattcac gtacaaccgc gcctgcgtga aagcgcagcg tcatcaggag
     5821 gaacactcgt tggcaacgac ccaggatttt cggaacgggt tcgcgttccg catggacggc
     5881 aacatctact tcattgtcga atttcagcac gtcaaacccg gcaagggtgg cgcattcgtg
     5941 cggacgcgcc tgaaaaacgt ccagacaggc gcggtgatcg accgtacctt caagtccggg
     6001 gaggcggtgg aagagatccg cgtggaaaac cgcgacatgc agtacatcta caaggagggc
     6061 gatcattacg tcttcatgga tacggagacg tatgagcaaa tccaccttcc gcacgaaatc
     6121 gtggatgaca tcgcggacct cctgaaggag aacgaagtcg tcacggtgat catcggagac
     6181 gacatgccgc ttctggtgga gctgccgaac catgtgacgc ttaccatcgc ggataccgac
     6241 ccgggcgtgc gcggcgatac cgcaaccggc ggcaccaaac cggcgaagct ggaaaccggg
     6301 gcggtggtgc aggtgccgtt attcctcggg gttggcgaaa aaatcagaat cgacacgaga
     6361 acacgccagt atctctcccg cgcctgatga agcgcgtctc ccnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nntcgcaggc attcccccac attgtcgctc ctgccccggt
     6541 tgtacagatt ccggactctc ccccggccgc ccctgcgaag gaaacacaag ccgctccggc
     6601 cgccgcgaaa gcgccggacg cgaacctcct cgaaatcaag gcgcccatgg tcggcacgtt
     6661 ctaccgcgcc ccggcccctg aagccgcgcc gtacatcgaa accggcgcgc acgttgccaa
     6721 agggcagatc atcggcatca tcgaggcaat gaagctgatg aatgagatac cggcggacgt
     6781 tgccggaacc gtggtcgaaa tcgccgtcga gaacgcggaa ccggtggaat tcggtcaggt
     6841 tctcctccgc gtgaagccgg actagcgcct atgttcagca aaattctggt tgccaatcgc
     6901 ggcgaagtgg cgctgcggat cgtccgcgcg tgcagagaac tcaatatcgc gacggtcgcc
     6961 gtctactccg aggcggacgc cagttccctc catgttcggt ttgccgatga agcagtctgc
     7021 atcggccccc ccgatgcccg gcagagttac ctcgatcccg cccggattat tgccgcggcg
     7081 gaggtgaccg gggccgaagc catccacccc ggatacggtt ttctggcaga aaatgcccga
     7141 tttgcggaca tctgcgcgga atgcggattc gtcttcatcg ggccgaccgg ggacatgatc
     7201 cgcatgatgg gcgacaaagc cgtggccaag aagaccatgg cggacgccgg ggtgccggtg
     7261 gtgccgggtt ccgacggccc gctgaaatcc gcggaacagg cgctcgccct tgcaaaggac
     7321 cacgggtacc cggtgatgct gaaagccgtc gcgggtggcg gcggaaaggg catccggatc
     7381 gtcacgtctc cggaaggtct tgtccgggcg tacgaccttg cccggaatga ggccgagact
     7441 gcgttcaaac tcggtgacct ctacctcgag aagttcgtgg accacgcccg ccacgtcgag
     7501 attcagatac tcggcgattc agagggcaac gtcgtacacc ttggtgagcg cgattgcacg
     7561 gtgcaacggc ggcgccagaa gctgattgag gaatccccct cgccggcggt tacccgcgat
     7621 ctgcgcgacc gaatggccag cgccgccgtg gcgggcgccg cgagcgtcgg gtacagaaac
     7681 gccggcacca tcgagttcct tctcgcgccc gaccagcagt tctatttcat ggagatgaac
     7741 acccgcgttc aggtggagca cccggttacc gagatggtga cgggaaccga tatcgtcaag
     7801 gagcagattc gcatcgcagc ggggctcccg ctcagtttcc ggcaggaaga tatcatcctc
     7861 caggggcacg cgatcgagtg ccgcatcaac gcggaagacg tcgcgcacga cttccgcccg
     7921 acgcccggcc gaatcaccga atggcatgtc ccgggcggaa tcggcgtccg tattgacacc
     7981 cacgcgtacg cgcattacgc cgtgccgccc tactacgatt ccatgctggc caagctcatc
     8041 gtccatgcgc cgacgcgggc ggaggcgatc gtacgcatga agcgggcgct cggagagttc
     8101 ttcgttgaag gcgtgccgac gacaatcccg ttccacagtc gcgtcatgag tgactcggtg
     8161 ttccagaacg ggcactacga tatggggtat gtggaagact tcttcacgcg aacagaaatc
     8221 tgaggaggac tgaccgtgga tttcccggat gatctggcgt acacccgcga tcatgaatgg
     8281 ttgcggttgg aaggagaaac cggcactgcc gggatcaccg attacgcgca acgggaactg
     8341 acggacatcg tgtttgttga attcgtcgcg ccgggtaccg ttgtcaggca gggcgaacgg
     8401 ttcggcaccg tcgaggcaat caaggcggtc agcgacctgt tcagcccggt ggacggagag
     8461 atcgtggagg tcaacaccgg gctgtcgacg caccccgagt ggatcaacca ggacccgtac
     8521 ggcaagggct ggatggtgaa aatcagggtg agcgatccgg cgcaagtccg caccctgctt
     8581 tccgccgccg actaccgcac gctcgtcgca agcggctgaa aaggcggttt gtgcatggca
     8641 gatccgcacc cgttcattcc ctgcaccgat tccgacctcg ccgaaatgct cgcggccatc
     8701 ggcgtccgct cgttcgaaga cctcgtcgcc cctgttccgg acgcgatccg tgcggcaagc
     8761 gcgcttaatc ttcctccacg gctctccgaa cacgaagcgc tcagccttct tcgtcggcgc
     8821 gcgcgggaaa atgccccgga cgccgtctgt ttcctcggcg ccggcatgta cgaccggatc
     8881 atcccggcgg cgattgacgc ggtagttcag cgctccgagt tcaccaccgc gtacacgccc
     8941 taccagccgg aagtgagcca gggcaccctg caggccatct acgagttcca gacgatggtg
     9001 tgcgagctgt tcggcatgga tgtggcgaac gcctcggtgt acgatggcgc gtcggcgctg
     9061 acggaggcga tactgctttc ccacgcgcat acaggccgcc gaaaggttgt cttcgccggg
     9121 ccggtgcacc cgcggtaccg ctctgccttc tcttctatca ccgtgggtat cgggttggaa
     9181 agcgttgagg cgccgttttc cgacggcggc gccgacgctg ccgcggtggc ggagcgggtg
     9241 agcggggaaa ccgcgtgcgt cgtcgtgcag cagccgaatt tctacggcgt catcgaggac
     9301 attgcggccc ttgcagcaat cgcgcatcgc gagggtgcgc tccttgtcgt atctgccgat
     9361 ccggtggcgc tgtcccttct tgaagcgccc ggaaaacagg gcgcggacgt cgtggtgggc
     9421 gaagggcagc ccctcggcat ccccatgagc ttcggcggac cggccctcgg cctgttcgcg
     9481 gtgcgggatg aactgaaacg ccgccttccg ggtcggctcg tcggccgcac ggtggatacc
     9541 cgcgggcaac aggcgttcgt gctgaccctc cagacccgcg agcagcacat ccggcgggaa
     9601 aaagccacca gcaacatctg ctcgaaccag aacctgatgg ccatcgcggc aacgatgtat
     9661 ctcaccctga tgggccccgc cggcctgcgc acggttgccg aaaactccct tcagaacgct
     9721 cactacgccg cggaccagat cgaagcgcgc acggtgttcc gccaggtgca cccccggccg
     9781 tttttcgatg aattcgtggt gaagtgcccc gttccggcgc gggaggttgt cgatcgcttg
     9841 gttccgcgcg gcatccttgc aggcgtggat atggcacagt tcggcggcga cccggcacac
     9901 ctcatgatcg ccgtcactga aaagcgcacg aaggaggaga tcgacgcgct ggtcgcggcg
     9961 ctgggggaga tcgggtaaga tctgccccgg tggccggcaa tcgaacatga ccccgtaccg
    10021 tgcaccccgc gacgaggatt aattcccaca ccgtcagccg ccgtaccttg cgcagaactg
    10081 cgggcgggcc gtatcaagtg gtgatgaaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn gcgaaccggg ctatgctatc ccgcgccgtt ggcgcctggc acccgccttt
    10261 gcggccgccg gaaggcctga agggccgaca tgactgagcc cggctgaacg ggccgggcgg
    10321 cctggcaagc cgcgggcagg cactcggacc gcgttctgga ttcccgcttt cacgtcgggc
    10381 tcggttgagc aacagggaat gacgatggaa cgcgcgggtc atcgagtgcg gcatcgccgc
    10441 gtatcgagat gacctgcgcg ttggccatct tgaccgattc gtaaccctgc acacataccc
    10501 cgcgaggagc caccatgaac ttccgtccgc gcaacccgaa ccgccacctg tgggacacct
    10561 ggttctacga ccacaacggc gagtaccacc tcttctacct ctccaaccgg atcgccgacg
    10621 acggcagatg gattccctgg gacgccttct ccctcgccac cagccgcgac ctgctccact
    10681 gggaggaaaa gggcgaggtg ttccacaagt cggaaaatcc cgcggactgg gatagcgagc
    10741 tcatcaccac gggcgatatc atccgctggc gtgggcgctg gtatatgacc taccgcgcca
    10801 cgcaggaccg cgtggagaag aacaacatca tcgtctcgga tgacctgctc aactggcagc
    10861 gaatcagccc gtcgccggtg ctggtaacga gcgagccgta tgagcacgac cccgcgcaga
    10921 cgatcaacaa ctacgtcgag atgcgggatg tgacgatcac cgagctaccc gacggcacgc
    10981 tggagggcgt gttcaccgcg cggctgaact cagggccgca tacggggcgc ggggtgctgg
    11041 cgcgggcgtt ttcggcggac atgctccact ggcgcatggg cgagccgctc ttcgcgccgg
    11101 gccggttcaa cgtcatcgag gtgcccagcc ggttccgcca cggcgggcgg cactacctgc
    11161 tgttctgcgt gaacagccac ctcgagtcgg cgctcgagaa ccgcaactac ccgtccatga
    11221 cctggtgcac ggggtacgcg gtgtccgacc ggtgtgatgg cgggtttgac tacgtgccgg
    11281 agcaggtgct cggtatcggc gatgcgtacg tgggcagact ggtgaacgcg ggcggggaaa
    11341 cgcttttctt ccagcacctg tgcggcagac ggccagcctt cgcgcttccg aagagcgtgt
    11401 cgttcggcgc cgacggcacc atccggctcg gatactggcg aggcgttgat tcgctcatcc
    11461 gtggcgaagg gattcggggc ttctcgacgc tccaattctt cgaagatggc ggcaaaacgg
    11521 tgtgcggcgg caacatcgcc ccggagacct gcacgctcga aaatgccgct ggcttcgggg
    11581 acgcctacct gccgggtgcc ttctccgact tcgtgttgga cgtggatatc cagttcgacc
    11641 ggtgcgtgtc ggcaggtgtg ctgctgaacg cggacgtgcc gtcgcgcggg gcagtcatgg
    11701 cgacgcttcg ctccgaccgg ggcgcgtttc aggtgggccg ctcgcggaag gtgtacaacc
    11761 acctcgcccc gaacggagtc cggcacgaat acctgagcga cgcattgaag cgggggaccg
    11821 tgcacctgaa ggtcgtgtcg cgctcggaag gcttcgactg gta
//
LOCUS       DAOSOO010000086        11803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5393_length_11803_cov_5.591164, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000086 DAOSOO010000000
VERSION     DAOSOO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_11803_cov_5.591164"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLT86_07680"
     CDS             <1..735
                     /locus_tag="PLT86_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36529.1"
                     /translation="VIAITTARIVAATGLLWVHDYFVPMHGMTVIVGSARVDPRTWTN
                     MGFVDFSTLSNRANIMPQLLDGMKIARQTSIRQGHYFLGVGLGLVTAVFVSFAMILWL
                     AYTYGGANLEPWHFFNGGDWLFNRTAGFQRYHVYTHWTAISLMGAGAVFMSVLLYLHR
                     TFLWWPIYPLGFLIGDTVASNEIWFPVFLGWVIKAAIIRFSGASAYNRLKNGALGLIL
                     GEFLSVGLWLVIDGFSGVTLHRVFPK"
     gene            824..1744
                     /locus_tag="PLT86_07685"
     CDS             824..1744
                     /locus_tag="PLT86_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT36530.1"
                     /translation="MGLSRRDFLKAGAMAAGGLVLPKWAYSAVSRAATSPDVVHVTGG
                     TVELAVRTSIDRLGGIGAFVRRGQKVLIVPNAGFPNPAEMATTTDPETIRAIATLCKE
                     AGASSITVSSYPVREPDLCFERSGIADLARMNGVNVVPLTSGSAYVPLRIPDGIDARE
                     VEVATVVRQSDVLIAVPVAKSHSSAGVSFAMKGMMGLIRSRGPFHARYDLHQAIVDLS
                     KGIKAQLVVVDARRALVTRGPGGPGRVETPNAIVAGRNQTTVDAYTVSLARWYNRAFT
                     ADQVRHLRLAGEQGLGLTDLSAMNVVRVTL"
     gene            1773..3398
                     /locus_tag="PLT86_07690"
     CDS             1773..3398
                     /locus_tag="PLT86_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT36531.1"
                     /translation="MNNLRRAVQLIAFAVFLAGTETYWLEYDPLISIASLVSREFSWG
                     LVLRGLLLASATLLIGRFFCGWLCPLGTFLDAAHRCGVRPSKDATRENGTRARWKFVF
                     LAFLIVAAAFGTTTFVLFDPLVLFSRTRELVLIPAAAWVGNGVVRAVRLVPGQESFAF
                     RAVENPPAYGFLTLTAAVFAGILILEVIERRFWCRNLCPLGAFLNLLSRTGLVRRSVE
                     SSCSRCARCERDCPTGAIPVNDFTKTRRGECIQCLRCQRACSKGTVRFVFGGPPQIRG
                     FEPRRRQVLLAAGAGVAAGFLGDASTKASTRPGAHLRPPGAIPEEEFLSRCTRCLICV
                     DACPTHGLQPSGLEIGWEGLWTPKLVPASGGCEEPCNACTQICPTLAIRRLSHEEKSF
                     AKIGTARIVKERCVAWEELKACLVCDEVCPYDAIEFLVVSDHRGTQNRPVVHADKCMG
                     CGLCEHACPVREPRAIVVDNYGEERIGSGSYITPAKQAARKVGDDRATDYFREFRAGA
                     GVPADSVRGAGSTPAAGADTIFEEPLPPGFVNP"
     gene            complement(3405..4406)
                     /locus_tag="PLT86_07695"
     CDS             complement(3405..4406)
                     /locus_tag="PLT86_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOT36532.1"
                     /translation="MAHAIAVLPQKDLRNALFSEKAWKELGTLGRVTYEPDADRPPDD
                     SRVRALLADATICITSWGSPNLGESAIENASALKLVAHAAGSVKPIVSPALWKRGVRV
                     TSAAAAIALGVAETTLGWIIIAAKRGILASQVTRAGGWLKDMAFPPGDLPGKVIGIVG
                     ASHVGRNVIRLLKPFPVTVMVYDPYLSDDDATALGVVKSELDDLMMRSDIVSLHAPKT
                     EETHHMINARNLALLRDGATLINTARGALIDEAAFVEHLRQGRTWAILDVTDPEPPPP
                     DHPFRSLPNVVLTPHFAGTVESGRSRIGDCVLEEIKRFIAGEPLHYEVHEEMLPRIG"
     gene            4573..5196
                     /locus_tag="PLT86_07700"
     CDS             4573..5196
                     /locus_tag="PLT86_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT36533.1"
                     /translation="MISLAGVTAVLVVIAALRSLEAASDQEIVSGVLGGNTDDFEGLV
                     VRYRRQVFRIALRVTRDRDNAADVAQEAFVRAFVALKQYDANRPFGAWISRITLNTAL
                     TLCSSRKKQGDERRTATLDNSNAADAFSTVARTEFETAVRAAIAGLPDGIRETFELRA
                     YADLSYDEIAHELGVPRGTVMSRLARARERLREVLEQQGFGRESEAV"
     gene            5201..6184
                     /locus_tag="PLT86_07705"
     CDS             5201..6184
                     /locus_tag="PLT86_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT36534.1"
                     /translation="MSAPGRGENICLNMRDLLSAYLDGETTPEETARVDAHLNTCEAC
                     RVVLARYRTIERAIAGEVPGADPAPQDSAVFTDAVMSRIREVADRPTLKAHILKWRTP
                     VVSALAIAATVVLALYLRPRTPEEPIHPVPTTTVPSPQERRETAQIASKPSERRPLTS
                     GGAPTPRTRAQIAEPPVVASAELGQAMGDIAPDETSIQDAKAKPARGGVSFVQDFYAT
                     RAAQAITGAAPAKSDSVVPDLRRKADAMRTELVSANETNSVDSLKLLLGDVLARIAER
                     TGEPLDVRAALTFWRQEYAVLAKQSGDSVVQSRVSRLEGILRSAQPKGSFE"
     gene            complement(6129..6914)
                     /locus_tag="PLT86_07710"
     CDS             complement(6129..6914)
                     /locus_tag="PLT86_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOT36535.1"
                     /translation="MSERFETEFSVRSYELDAALDAPPASFLRWFQEAAIRASAAYGY
                     DEERYEQLGTAWFMKESHLLIRSQPRTGELVRVATWVGDITRVNSHRQYLAFGSDGRM
                     LARGEANWVYVDRKTSRPRRLEREILDAFQQKSEYALEDRDWGAAVLSRTYPADPQFT
                     TTHVVRWTELDAAGHVNNAVYADWIRDDCAAFRPEARIVRLRLNYRQPALLGESLVRE
                     LVPLEETVWGYRIRRCESEETISLAVIQTTLSVVPNEECLRDG"
     gene            7373..8665
                     /gene="hflX"
                     /locus_tag="PLT86_07715"
     CDS             7373..8665
                     /gene="hflX"
                     /locus_tag="PLT86_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT36536.1"
                     /translation="MKEYYYTSEAFKPERAIVVGLQLGLQSAEEAQSSLEELEALADT
                     AGASVVARHFQHRAAPDPTFFVGKGKVGEIARDVEERQADLIIFDDDLSPAQARNVER
                     ECGIRVLDRSGLILDIFAKAAKTREARLQVECAQLEYLLPRLTRQWLHLERQAGTGGG
                     SSGAPIGLRGPGETQLETDRRLISARIAHLKRELEHIDRIRSTQRARRDRVYRCALVG
                     YTNAGKSTLMHALTGADVLIDDRLFATLDSTTRAFNLAPNKPVVLTDTVGFIRKLPHG
                     LIASFRSTLAEAREADLVLHVVDVSGPAAHEQIAAVNEVLSELDIHPSNTLLVMNKSD
                     ACVDPDDAARLAAGRPYVSVSALTGEGLDRLKEAIAANIEADMVLVEVRLPQSEGRLI
                     SEIHSRGEVLARTYEPDAVILHARVRRSDAEFLTRHSV"
     gene            8712..8906
                     /locus_tag="PLT86_07720"
     CDS             8712..8906
                     /locus_tag="PLT86_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36537.1"
                     /translation="MKLTREQLADLKGKSPEGIYQHIRQFVRQELGVVDKDELSEAVD
                     DAIRAGLLDEDDLRKLGDDR"
     gene            9163..10500
                     /locus_tag="PLT86_07725"
     CDS             9163..10500
                     /locus_tag="PLT86_07725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT36538.1"
                     /translation="MRGEPVPLQPTRPNVSFAHQLWPGDCLTLLLAGFVASVLTGNFV
                     PRTNLSVLGTLVAMAGIIAVLLTSRAYEIQFPMAGTTSTYHAMRVLLWTVPPGGALWF
                     VFEPRVETVFAVFCYGIIAIFGMTLNRVIVFPRVRAAAKSRVVVCGLNGDAVCVAQRF
                     ATDQDTMTPTIVGLLTPEAIENQPQRPRLGSWEDMQRLFSIGMFDEVVFSVPQSSPAS
                     LPLAATAVKHRIPFRFEPHERGSHFSPRDHSVDLTIEPPMGKVGRAIKRAVDLVGATL
                     LLVVTAPLYLAIAVMIKRDSQGPVLFAQKRVGLHGKLFTMYKFRTMAVGTSAYDFSPR
                     EGSDSRVTRAGRFLRKTSLDELPQLFNVIRGEMSLVGPRPEMPFIVGQYGPLEDARHK
                     VKPGMTGIWQTSRHRNDPIHENIHYDLFYVQNQSLILDLVILTRTAFLAFRGV"
     gene            10621..11568
                     /locus_tag="PLT86_07730"
     CDS             10621..11568
                     /locus_tag="PLT86_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT36539.1"
                     /translation="MHAPEPITMITFERWPEPWMEELRAAAPGSTIFRPQSREGIDWA
                     SLDALNGGINKDILAKAARLRWFHSPAAGVEGYLFPEMVESPVVLTNSKGVYSIFIAE
                     LAMGHLLALTTQVHANTVRPDRLTWLRDTLRGFELYGATAVVIGLGNIGSEVADRAKA
                     FRMRVIGVDPVRKAAAGVELVRPDRIDDVLPLADVIFITAPATTARKLFDRERLQSLK
                     KGAYLINSSRGPVVDTDALVEALRSGHLGGAGLDVTDPEPLPADHPLWTLPNVTITPH
                     IGGMTDAVDRRRFVILRENLWRFVRGVPLLNVVNKKRGF"
BASE COUNT         2291 a         3410 c         3621 g         2481 t
ORIGIN      
        1 gtgatcgcaa tcacgacggc acgtatcgtt gcggcaacgg ggctgctgtg ggtgcacgac
       61 tactttgttc cgatgcacgg catgacggtt atcgtgggtt cggcccgcgt agaccctcgg
      121 acgtggacga acatggggtt cgtggatttc tccacattga gcaaccgggc gaacatcatg
      181 ccccagcttc tcgacgggat gaagattgcg cggcagacga gtatcaggca gggacactac
      241 ttcctgggcg ttggccttgg cctcgtcacc gcagtttttg tctcgttcgc gatgattctc
      301 tggctggcgt acacgtatgg tggcgcgaat ctggagccat ggcatttctt caacggcgga
      361 gactggcttt tcaatcgaac ggcgggattt cagcgttacc acgtgtacac tcactggacg
      421 gcaatcagcc tgatgggcgc gggtgccgtg ttcatgagtg tgctgctgta tctccatcgc
      481 acgtttctct ggtggcccat ttacccgctg ggtttcctga tcggcgatac cgtggcgtcc
      541 aatgaaatct ggttcccggt ttttctggga tgggtcatta aggcggcgat tatccgtttc
      601 agcggtgcaa gcgcctacaa ccgattgaag aacggggccc ttggtcttat tctcggggaa
      661 tttctcagcg tagggctctg gcttgtgatt gacgggttct ccggagttac cttacaccgg
      721 gtatttccga aataggaccg gccagggctg caacgcccgc gactctacat cggcgctgtt
      781 cgccgtcgcg cgcaccggcg tggcaggaaa aaggagcatt cccatgggac tttcgcggcg
      841 cgatttcctc aaagcaggcg cgatggcagc cggcgggctg gtactgccga aatgggcgta
      901 ttcggcagtt tcgcgggcgg ccacttcacc cgatgtggtg cacgtaaccg gtggaaccgt
      961 cgaactggcg gtgcggactt ccatcgatcg gctgggggga atcggtgcct tcgtacggcg
     1021 cggccagaaa gtgctcatag tccccaacgc gggatttcca aaccccgcag agatggcaac
     1081 caccacagat cctgagacga tccgcgcaat cgctaccctg tgcaaggaag cgggtgcgtc
     1141 atccatcaca gtctccagct accccgttcg cgaaccggac ctttgtttcg aacgcagcgg
     1201 aatcgccgac ctcgcgcgca tgaacggcgt gaatgtagtt cccctcacct ccgggagcgc
     1261 atacgtgccg cttcgtattc ccgatgggat tgacgccagg gaagtggaag ttgccacggt
     1321 cgtgcgccag agtgacgtgc tgatcgcagt tcccgttgcg aaatcccatt cttccgccgg
     1381 cgtctcgttc gcgatgaagg ggatgatggg gctgattcgc tctcgcgggc cttttcacgc
     1441 ccgttatgac ctccatcagg cgatcgtgga cctgagcaag ggcatcaaag cacaactggt
     1501 cgtggttgac gcccgcaggg cgctggtgac ccgcgggccg ggagggccgg gccgcgtgga
     1561 gaccccgaac gccattgttg cggggcgcaa tcaaaccaca gttgatgcct acacggtgag
     1621 cctggcgcgg tggtacaacc gcgcgttcac cgccgatcag gtccggcatc ttcgattggc
     1681 gggggaacag ggcctagggc tgacggacct ttccgcaatg aacgtcgttc gagtgaccct
     1741 ctgatcgccg cgcctgctac ttgaggcacc cggtgaacaa cctgcgacgc gccgttcagt
     1801 tgattgcctt cgcggtattc ctcgcgggta cggaaaccta ctggctcgaa tatgaccctc
     1861 tcatttcaat cgcaagtctt gtttcgcgcg aattctcgtg ggggcttgtt cttcgagggc
     1921 tgcttctcgc cagcgcaaca cttctcatcg ggcgcttttt ctgtggctgg ttgtgtccgc
     1981 ttggcacatt tctggacgcg gcgcatcgct gcggtgtacg gccttcgaag gatgcgacgc
     2041 gcgaaaacgg tacgcgggcg cgatggaaat tcgtcttcct cgcctttttg atcgtcgcgg
     2101 cggcgttcgg tacaaccaca ttcgtccttt ttgaccccct tgtccttttc tctcgaacac
     2161 gggagctcgt cctcattcca gcggctgcct gggtgggtaa cggagtagtt cgagcggtca
     2221 gattggtacc ggggcaggaa tcgttcgcgt ttcgtgccgt cgaaaacccg ccggcgtacg
     2281 ggttcctcac gctgacagcc gccgtctttg cggggatcct gattcttgag gtaatcgaac
     2341 gtcggttctg gtgccgcaat ctgtgcccgt tgggcgcgtt tctgaatctc ctttcccgca
     2401 cgggcttggt acggcgctca gtggagagct cctgctcccg atgtgctcga tgcgagcgag
     2461 actgcccgac gggtgccatt cccgtgaacg atttcactaa aacccggcgt ggcgagtgca
     2521 tccagtgcct gaggtgtcaa cgtgcgtgca gcaagggtac cgtccggttt gtcttcgggg
     2581 ggccaccgca gatacgtggt tttgaacccc gcaggcgcca ggtgcttctg gcggcgggcg
     2641 caggcgtcgc cgccggattc ctcggcgatg cctccacgaa agccagcacg cggccgggtg
     2701 ctcaccttcg cccccccggc gcgattcccg aagaggaatt tctttcccgt tgcacccggt
     2761 gtctgatctg cgtggacgcc tgccccacgc acgggctcca gccaagtggc cttgagatcg
     2821 gatgggaagg gctgtggact ccgaagctgg tgcctgcgtc cggcgggtgc gaggagccgt
     2881 gcaacgcgtg cacacagata tgccccacac tggcgattcg aaggctttct cacgaggaga
     2941 agtcgttcgc gaagatcggc acggcgcgca ttgtgaagga gcggtgcgtc gcatgggaag
     3001 aactgaaagc ctgtcttgtc tgtgatgaag tctgtcctta tgacgccatt gaattcctgg
     3061 tggtttcgga ccaccgggga acgcagaatc gtccggtggt tcatgccgac aaatgcatgg
     3121 ggtgtggtct ttgtgaacac gcgtgtcccg ttcgtgaacc gcgagcgata gttgtcgata
     3181 actatggaga agagcggatt ggcagcgggt cgtacatcac accggcgaag caagccgcac
     3241 ggaaggtggg agacgacagg gcaacagact acttccgcga gtttcgggca ggcgcaggag
     3301 tccctgccga cagcgtccgc ggcgctggtt ccactccggc ggcgggcgca gacaccattt
     3361 ttgaggagcc gttgccgccg gggtttgtaa acccttgagg tcctctaccc gatccttgga
     3421 agcatctcct cgtgcacctc gtaatggagt ggctcgcctg caataaaccg tttgatttcc
     3481 tcgagcacac agtctccgat acgagaacgg ccactttcta ccgttcccgc gaaatgaggc
     3541 gtcagaacaa catttggcag agatcggaac gggtgatcgg ggggcggagg ttcgggatcg
     3601 gtcacgtcga ggatggccca ggttctgcct tgcctcaggt gctcgacgaa tgctgcttcg
     3661 tcaatcagcg cccctcgagc tgtgttgata agggttgcgc cgtcacgcaa aagcgccaga
     3721 ttacgcgcat tgatcatatg atgtgtttct tcggtttttg gagcgtgaag cgaaacgata
     3781 tccgagcgca tcatcagatc gtcgagttct gacttgacca cgccaagtgc cgtcgcgtcg
     3841 tcatcagaga ggtaaggatc atacaccatc actgtaacgg ggaacggctt gagcaaccga
     3901 atgacgttcc ggccgacatg actcgcgccg acgatgccaa taactttgcc gggaagatca
     3961 cccgggggga acgccatatc cttcagccat ccccccgccc tcgtaacctg gctggcgagg
     4021 atgccgcgtt tggccgcgat gattatccaa ccgagggtgg tctccgcaac accgagcgcg
     4081 atcgcggcgg cagcgctggt gacacgaacc ccacgtttcc agagagcggg cgagacgatt
     4141 ggctttactg atccggccgc atgagcaacc agcttcaggg cacttgcgtt ttcgatggcg
     4201 gactcgccaa gattcgggct tccccaggat gtgatacaga tggtggcatc cgcaagaaga
     4261 gcccgaacac gggaatcatc aggaggccgg tccgcgtccg gttcgtacgt tacgcgtccc
     4321 agtgttccca gttccttcca cgccttttca ctgaagagcg cgtttcgaag atccttctga
     4381 ggaagaacgg cgatagcgtg cgccatggaa gagcctccaa gggaaaaatc tcgtctattg
     4441 tccgacacca ctccgtgaag ctaattccgt atcttttccg acacaacgtc atttcttacg
     4501 gtcaatttct tttcagccgc agtgacggga atattccgcg cgcggcgaaa gtaatctgga
     4561 gtaacgaaac ccgtgatcag cctcgctggc gtgacggccg ttcttgtcgt gattgcagcc
     4621 ttgcgcagcc tggaagcggc ctcggatcag gagattgttt caggcgttct cggcggcaat
     4681 accgatgact ttgaaggatt ggttgtccgg tatcggcggc aggtgtttcg aatcgcgctg
     4741 cgcgtgacca gggaccgcga taatgcggcg gatgtcgctc aggaggcgtt cgtgcgggct
     4801 ttcgtggcat tgaagcaata cgacgcaaac cgccctttcg gcgcatggat ctcacgcata
     4861 acactcaata cggccttgac gctttgcagc agccgaaaga aacagggcga tgagcggaga
     4921 acagccaccc tggacaactc aaatgcggcg gacgcgtttt ccaccgttgc gaggaccgag
     4981 ttcgaaacag cggttcgagc ggcaatcgcg ggtctgccgg acggaatacg cgaaaccttt
     5041 gaactccggg cgtacgcgga cctcagttat gatgagattg cgcacgagtt gggcgttcca
     5101 cggggtacgg tgatgtcaag gctggcacgc gcaagagagc gtctccgcga agtactcgaa
     5161 caacaaggtt tcggcagaga gagcgaggcg gtctagaacc atgagtgccc ccgggagagg
     5221 cgagaatatc tgcctgaaca tgcgcgacct tttgagcgcg tacctcgacg gcgagaccac
     5281 tccggaggaa accgcacgag tagacgcgca cctcaacacg tgcgaggcat gccgggttgt
     5341 gctcgctcgc taccgcacca ttgagcgcgc gatcgccggg gaggttcccg gtgctgatcc
     5401 cgctccgcag gattcggcgg tgttcacgga cgctgtcatg tcgcggatac gcgaagtggc
     5461 agacaggccg acccttaagg ctcacatcct caaatggcgc actcccgtag tttcagcgct
     5521 cgcgatcgcg gccactgtgg tactggcgct ctacctccgc ccgcggaccc cagaggaacc
     5581 gattcatccg gtgcccacta caacagttcc ctcccctcag gaacggcggg agaccgcgca
     5641 gattgcatcc aaaccttccg agcggaggcc gctcacatca ggcggggcgc ccaccccccg
     5701 cacgcgggca cagattgcag aaccgcctgt ggtggcttcc gcggagcttg gccaggcgat
     5761 gggcgacatc gccccggatg agacatccat tcaggacgca aaagcgaaac cggcccgcgg
     5821 gggcgtgagt tttgttcagg atttctatgc aacccgtgcg gcacaggcga tcacgggcgc
     5881 cgcccccgcg aaaagcgact ctgttgtgcc ggatctgagg aggaaagcgg acgcgatgcg
     5941 aaccgagctc gtttcggcca acgagacgaa ttccgtggat tcgctcaaac tcctgctggg
     6001 cgatgttctc gcgcggatag cggaaagaac cggagaaccg cttgatgtac gcgccgcgct
     6061 cacgttctgg cgtcaggaat acgctgttct ggcgaagcag agcggcgatt ccgtagttca
     6121 gtctcgcgtc agccgtctcg aaggcattct tcgttcggca caaccgaaag ggtcgtttga
     6181 atgaccgcga gactgattgt ttcctcggac tcgcagcggc ggatgcggta tccccacaca
     6241 gtttcttcca ggggcacaag ttcccgcacc agcgattccc ccagaagcgc aggttgccgg
     6301 tagttcaggc ggaggcgtac gattcgtgcc tcgggacgaa acgccgcgca gtcatcccgt
     6361 atccagtctg cgtacacggc gttgttcacg tggccggccg cgtcaagttc cgtccaccgt
     6421 acaacgtggg tggtggtgaa ctgcggatcg gcagggtacg tacgggaaag aactgccgca
     6481 ccccaatcac gatcctcgag ggcatactcg cttttctgtt ggaatgcgtc aaggatttcc
     6541 cgctcgagtc ggcgcggacg gctggttttt ctatccacgt acacccagtt cgcttctccg
     6601 cgggccaaca ttctcccgtc agatccgaag gcgagatact ggcgatggga gttgacccgc
     6661 gttatgtcgc ctacccacgt ggctactcga acgagttctc ctgtacgggg ctgagaacgg
     6721 atcaacaggt gggattcttt catgaaccag gcagtgccca gttgttcgta ccgttcctcg
     6781 tcgtacccgt acgccgctga ggcacggatt gccgcttcct gaaaccaacg aaggaacgaa
     6841 gcgggcggag cgtccagtgc tgcgtccagt tcatagctcc ggacggaaaa ctccgtttcg
     6901 aatcgctcag acatgcgtgc agcctcgttt cattcaatac actcatagtg ctatacggct
     6961 gtaccgaagg tcgttcaaat ggtaccgaat gtcatacccc cgaatgggcc ctgatcaatg
     7021 gccggggtcg atcagcgggt ccttttgcga gaacgaagac actatcgccg ccgaacgcca
     7081 gggtacgcca catgctatcc ttcgtaatca atcgccggat ctgttgccgt ccctcggatg
     7141 cgcgcaggca ggttcgcgag cttatcttgt aatgggggat acgctttgta cgttcttgtt
     7201 tccgatatgg ccggcaggga tcggcaggga taccaagata cgcgagatgc ctttccgtgg
     7261 cccccgatcc gccgacattg ttgcctccga atttcgcgca aaaacacagg cggaacggcg
     7321 cggctcggga aagaaggatc tcgcttccgg tgacactcgt tcaggaggtt gtgtgaagga
     7381 gtactactac acgtctgaag cgttcaaacc agagcgtgcg atcgtcgttg gtctacaact
     7441 gggacttcag agcgcggaag aggcacagag ctctcttgag gaactcgaag cgctggccga
     7501 cacggcgggg gcttctgtag ttgcacgcca tttccaacac cgggcggcgc cggatccgac
     7561 gttcttcgtg ggaaagggca aggtggggga gatcgcgcgg gacgtggagg agcgccaagc
     7621 tgacctcatc atcttcgatg acgatctctc gccggcacag gcaagaaacg tggagcgtga
     7681 gtgcggaatt cgggttctgg acaggagcgg gctgattctc gacattttcg ccaaggcggc
     7741 gaagacgcga gaagcccgcc tgcaggtcga atgcgcgcaa ctggaatacc tgttgccccg
     7801 gctgacgcgt cagtggctgc accttgagcg ccaagccgga acggggggcg gttccagcgg
     7861 agctcccata gggcttcgcg gcccgggcga aacccagttg gaaaccgacc ggaggttgat
     7921 aagcgcgcgc atcgcgcatc tgaagcggga acttgaacat atcgacagaa tccggagcac
     7981 tcaacgcgcc cgaagggacc gagtgtaccg ttgcgcgctg gtcgggtaca cgaacgcagg
     8041 caagtcaact ctcatgcatg cactcacggg tgcggacgtg ttgattgatg accgtctttt
     8101 cgccacactg gattccacca cacgggcatt caaccttgcg cccaacaaac cggtggtgct
     8161 tacggatacg gtgggcttca tcaggaagct accgcacggg ttaatcgcgt cgttccggag
     8221 tacactggcg gaggccagag aagctgatct ggtgctccac gtggtggatg tgagcggacc
     8281 ggcggcacac gagcagatag cggccgtgaa cgaggttctt tccgaactgg atatccaccc
     8341 gtcaaatacg ctcctcgtca tgaacaaatc ggatgcctgc gttgaccctg atgacgcggc
     8401 gcggttggcc gcgggaaggc cgtacgtttc tgtcagtgct ctcacgggcg aaggattgga
     8461 ccggctcaag gaggcgatcg ctgccaacat cgaggcggac atggtacttg tggaggttcg
     8521 gcttccccag tccgaaggga gattgatttc cgagatccat tcccggggcg aagtcctggc
     8581 tcgcacgtac gaacctgacg cagtaatcct tcacgcccgt gtacgccgct ctgacgccga
     8641 gttcctgact cgtcactccg tttgaccgca acaccagaca tggcaacgcg ctttcagtgc
     8701 aggaggcctt gatgaaactc acgcgcgaac agcttgccga tttgaaaggg aaatcgcccg
     8761 aaggaatcta tcaacacatc cggcaatttg tccgtcagga actcggggtg gtggacaagg
     8821 acgaactttc agaggcggtg gatgatgcca tccgggcggg tcttctcgac gaggacgacc
     8881 tgaggaaact gggcgacgac cgctgacggt cccgctggat atcgcacaca cgcctgatca
     8941 ctctgcgatc tctcttcgtg tgttggaacc tatccgcttc acatgtcggt ttcttttgac
     9001 aaaattctga aaacgtgtat ttttatcgtc actgattgac tgatccatcc ggtttggttt
     9061 cggggaatac agcgggttgt ttctcggccg ctcacgcgaa ttagacaggt aacacaggtg
     9121 ccgggtattg ccttacgacc ttccaagggg attctgggga gaatgcgggg agagcctgtt
     9181 cccctgcaac ccacccgtcc gaacgtttcg ttcgcgcatc agctttggcc gggtgattgc
     9241 cttaccctcc tgctggccgg tttcgttgcc agtgtgctta ctggcaactt cgtaccccgt
     9301 acgaacttga gtgttctcgg cacgctcgta gcgatggcgg gtatcatcgc cgtcctgctc
     9361 acgtcccgcg cgtacgagat ccagtttccg atggcgggaa ccacgagtac gtaccacgcc
     9421 atgcgtgtgc tcctgtggac cgttccaccc ggcggggcgc tctggttcgt tttcgagcca
     9481 cgagtcgaga ctgtgttcgc agtcttctgt tacggaatta tcgcgatctt cggcatgaca
     9541 ttgaaccggg tgatcgtatt cccccgcgtg cgcgctgcgg cgaaaagcag ggtggtcgtc
     9601 tgcgggttga atggtgacgc cgtctgcgtc gctcaacgct tcgccacgga ccaggacacc
     9661 atgacgccca cgatcgtagg gctcctgact ccggaggcga tcgagaacca gccgcagcgg
     9721 cctcgtctgg gctcgtggga ggatatgcag cgcctcttct cgataggcat gtttgacgaa
     9781 gtcgtgttca gcgtaccgca gtcatcaccc gcgtcactgc cgctggccgc aacggcggtg
     9841 aagcaccgga ttccatttcg ctttgagcca cacgaaaggg gctcgcattt cagtccccgt
     9901 gatcattcag tggatttgac aatcgagccg cccatgggga aggttgggag agcaatcaag
     9961 cgcgcggtcg atcttgtggg tgcaacgttg ttgctggtgg ttaccgcgcc cctttatctc
    10021 gcgatcgctg tgatgataaa gcgggattct caagggcctg tcctgtttgc acagaagcgc
    10081 gtgggattgc atggcaagtt gttcaccatg tacaagttcc gaacgatggc cgtgggaacc
    10141 tcggcttatg atttcagtcc cagagaagga tccgactcga gggtgacccg cgcggggagg
    10201 tttctgcgga aaaccagcct ggacgaacta ccccagttgt ttaacgtcat caggggtgag
    10261 atgagccttg tcggtccgcg cccggagatg cctttcatcg tcgggcaata cggtcccctg
    10321 gaggatgcgc gtcacaaagt gaaacccggg atgacgggga tttggcagac cagcagacac
    10381 cggaacgatc cgattcacga gaatatccac tacgacctgt tttacgtgca aaaccagtcc
    10441 ctgattctcg acctcgtcat tcttacgagg acggctttcc tcgcctttcg gggtgtatga
    10501 gcctcggtcg ctttggcttg tcgctcacct cgcacccttg ccccttctgc gcttcgtgaa
    10561 cattttgaac gtactccgca tccggcaaat ctcactcgct ttcaaggacg ggcacttccg
    10621 atgcatgcgc cagaacccat cacgatgatc acattcgagc ggtggccaga accctggatg
    10681 gaagaactcc gcgccgccgc gccgggatcg acgatattca gacctcaatc gagagaaggg
    10741 attgactggg catctctcga cgccctgaac ggcggcatta acaaggacat cctggcgaaa
    10801 gcagccaggc tcaggtggtt ccattcaccg gctgcgggtg tggaggggta tctgttccct
    10861 gagatggtcg agagtcccgt ggtccttacg aactcaaaag gcgtgtacag cattttcatc
    10921 gccgaactcg ccatggggca tctcctcgct ctgacgactc aggtgcacgc caatacggtg
    10981 cggcctgacc gcctaacctg gctccgggac acactgcgag gattcgaatt gtacggggcc
    11041 acggcggtgg tgatagggct ggggaacatc gggtcggagg tcgcggaccg tgcgaaagcc
    11101 ttcaggatgc gcgtcatcgg agttgacccg gtcaggaaag ctgccgcagg ggtggagctt
    11161 gtccgcccgg accggatcga cgatgtgttg cccctcgcgg acgtgatctt catcacggct
    11221 ccggcaacaa ccgcgcgcaa gctgtttgat cgagagcggc tccagagcct gaagaaaggc
    11281 gcatacctga tcaattcctc ccgcgggccc gtggtggaca ctgatgcgct cgtggaggcc
    11341 cttcgcagcg gacatctcgg cggtgccggg cttgatgtga cggatccaga acccttgccc
    11401 gcggatcacc ctctctggac acttcccaac gtcacgatca ctccccacat cggtggaatg
    11461 accgacgccg tcgatcgaag gcggttcgtc attctccggg agaacctgtg gagattcgtt
    11521 cggggtgttc cgctccttaa cgtggtgaac aagaagcgcg gtttttgatt tctggcgatt
    11581 tctcaccgga agcggctctg aaggcttttc agcgccgctt ttccgttggc gacgcgggag
    11641 aacgcatgcg tatcgcgata ggtggaatct atcacgaatc caattccttc tcggcgaggt
    11701 tgacaacgct cgaggatttc cggtcgtcga gtctgctgac cggtggtgcc atcattgact
    11761 actggcgggg cacgaactca gaggtcgcag gatttctgga cgg
//
LOCUS       DAOSOO010000087        11763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5419_length_11763_cov_5.457977, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000087 DAOSOO010000000
VERSION     DAOSOO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5419_length_11763_cov_5.457977"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLT86_07735"
     CDS             <1..427
                     /locus_tag="PLT86_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT36540.1"
                     /translation="AGKRDRALVLFREFLAEASVDNPNRVIARGKLKMAGQDGGEPEP
                     RITRPASPPLLTARPNPFNPTTSITYRVTAQGNVSVQVYNALGQRVRELVNTTAQPGV
                     YTVVWDGKDSAARPVGSGVYLVRLTTPTAVVTERVTLAR"
     gene            complement(553..1704)
                     /locus_tag="PLT86_07740"
     CDS             complement(553..1704)
                     /locus_tag="PLT86_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOT36541.1"
                     /translation="MRITDVKPLVLGTSWRNIIFIKVETDEGLTGIGEATIQNREEGV
                     LGYLEGAKRRHVIGSDPFNVEDLWLRMYRNDFWRNGVIATTVMSAVEIACWDIIGKAT
                     GQPVYRLLGGQAHEKLKAYANGWYTVEREPQQFAARAKMVTAKGYKALKVDPFGPGTY
                     EMSRQEIMKSLELVAAIRDAVGPDVEIFIEGHGRFSPRTAIQIGKQLEEFNPGWYEEP
                     VPPENAQALKQVHEQVNIPIASGERWFTRYQFPEFLNSGAVDIVQADIIHAGGFLEMK
                     KIAAMADAQYRVMAPHNSNGPVCTAASIHFGFCTTNYKVQEIFDDFVEPWVKEAVPGV
                     PNVVDGYVELPTGPGLGISLNEDLIKEHPYKEGFFNLFANDWQKRQFAK"
     gene            1944..3920
                     /locus_tag="PLT86_07745"
     CDS             1944..3920
                     /locus_tag="PLT86_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT36542.1"
                     /translation="MSPEIPPASLLSRAQKRLFGPPRHIKDPSLLHKMSLIPLLAWVG
                     LGADGLSSSAYGPEEAFRALGEHAYLALFIGLATALTVFIISFAYSRIIEHFPHGGGG
                     YVVATQMLGKNAGVISGSALLVDYVLTVTVSIASCVDAVFSYLPAPVHAHKVLAACLL
                     LLMMVILNIRGVKESITVLAPIFLVFIATHALMLLDGVFTHTQGFAPMVADVKRGISR
                     DVSTIGWIGVAAVFLRAYSLGGGTYTGIEAVSNGLQILRDPKVKNGKRTMLYMALSLA
                     FTASLLFLCYLLVSVKPVEGRTLNAVLADTLFADWRLGGTIAFVTIFSEGALLLVAAQ
                     AGFVDGPRVMANMALDSWFPRRFAALSQRLTMQNGIVLMGVAAFFLLLYTHGSVSALI
                     VMYSINVFVTFSLSQLGMSRYFINERRTMARWVNKLSVHLVALVLCVTILAVIVFEKF
                     AEGGWLTVLITAAVITACYWIHAHYDKVKKGVRELDEMLMDLPLSGPVYTAAPKHNDQ
                     TAIQLVSGYNGFGVHTLLTIARTFPNLYKNFVFVSIATVDSGLFKESDTVEALESKAQ
                     ADMERYVELARKLGFAAEWRTDVATDVVEGAVKVCREVVREFPRSTVFAGQITFRLEK
                     GYHRLLHNETSYSIQRLLQWAGITTVILPIRIKN"
     gene            complement(3999..4973)
                     /locus_tag="PLT86_07750"
     CDS             complement(3999..4973)
                     /locus_tag="PLT86_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36543.1"
                     /translation="MRVHIESVRCVFNNGEHNAFTDLCQFRGQLYLTFRSCPDGHMVF
                     PTSRIRVMRSSDGVTWEQVCEFGVPERDVRDPHFLVFGTRLFVYTGTWLCEENRDINH
                     HLGYCMWSDDGEVWRGPRFLEGTYGHYIWRAAAFGGKAYLCGRRKRAFARTADHGDGT
                     RITESALMESDEGFVYKPVGFFQHEYGDETAFLFEPDGSLLAIARDGAAKNALVCRSQ
                     PPFTEWSRVKLARQIGGPMLAKWAGGYLIGGRKSEPEKPSRTALWWLVDDTLEEIAEL
                     PSGGDTSYPGFVALGDSDGLLSFYSSHEGSGTGDPPSAIYLADLRIVA"
     gene            complement(4987..5886)
                     /locus_tag="PLT86_07755"
     CDS             complement(4987..5886)
                     /locus_tag="PLT86_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36544.1"
                     /translation="MTGTTSTERAITRRIARDTLTPVEMNHGDVLEFQLVSGDVVRIA
                     LVSTSAHVVRTTLKHLKVEEDAAQTLYRFNCVLRINGREHALEREIPSQRSFYEPWEI
                     DGVRIWFDAVDAIFEFLTEEHGECRPKKHARFAFQDATLRICPDSLHPWCPLPEGGLK
                     IEQCYRGEDCWMGPYNGASAHGGLDINHPRGTPLWAPIDIHDHSYFNSLAMGHNNNRW
                     RGIHRWPDGSEWILQAHHMTELTVPEHRPLKKGEQFAWGAGVLSGAVDHSHFVFKIRE
                     DDDIIALDPWILFWQMYRDQIAP"
     gene            complement(6179..10780)
                     /locus_tag="PLT86_07760"
     CDS             complement(6179..10780)
                     /locus_tag="PLT86_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT36545.1"
                     /translation="MTGDLAKPGFRGTELGLADPEPLFPLRSRKPSASALPEQVGAWF
                     RYRISNELSPQQVADSLNRIPGVVAQPNYLYRMLGTAPNDPGWQNQWGAQRIRLDEVW
                     QQVQADAAKCPIIGIIDSGVDYRHPDLEANIWHNAIEQAGLPGVDDDGNGYVDDFVGW
                     DFTDSPGLSGLGDYLDRDNDPFDEMGHGTQVAGVAAAVVNNGVGIAGTAPNARIMCLR
                     AGAQLINGSGYLEEDDIAAAVVYAVENGADIINMSFGDVVSTPLMADIVAYATAARVV
                     LVAAAGNENTPSLLFPAAYDETIAVGATGENDARATFSSYGENLDLCAPGVSIYTTNT
                     WGAYRRNGGTSFSAPFVSGAAAILLSRAPHLSPTEVRSILCRGVVDIGPPGWDEQTGH
                     GRLDLPTLLSVAQDPVAQILTPGRTKEVRNAVRWAVHVRGTPPVAWVLSYGVGAVPRV
                     WTTCASGVLETAGDTLRGTYSTIDHPDTLFCLRLTVTDLLGRTGEDRTLYSLDRSPPV
                     FRSQPAVLPRWDGDTRRIFLQWETDDLTTGTVRIWRGAVGGSSPLEYSVSATDRAHFE
                     EIPRQDVGVGPFTVQVVARNASGMETESAFSTGTASAYTIPRSGWDEEDVRAGGILMP
                     AGTGMPPLPDIDGNGIPEAVIKLAHRAPYDTVSFFELPPGSPPVLRTESPVKMRPTAV
                     VDIDGNAKLDLIGFDYFDSAFRVRVVEIGQSSPWWERTSLVGPSVADVDGDGRAEIIA
                     VVDSNRALLHVYEAAGPGRIELKAILQSPVNGYEGQFGIWRAAGDFDGTGRNSIVAGT
                     VGGAVVLFPSVGDDAFGVPRVIEGQGDATRVCAIGDVNDDGRDDFAIVRHLENTKFTA
                     DVAAFRLEVRTGGTTPFFVREFHDPRTEGNGFSSGHMGGRRVVVLATPPHLYVLDPTG
                     PGVDGFLYYDETDTPSQPLIADIDGQGGAELLFPRGGALAVLRALASPRAPAAPRNVS
                     VRVRDSISVVVSWNAEPGLHYRIWTGVGRDAPLSLRTEFADPVPPVVLTFTPPVQSEI
                     RVAVQAVNPAIADSIGGVSDAIVVRPHTGPQALCAARVSPSGVDVRFDLPLSALETTE
                     RDFLLRAGAQEHRPSSLVWDRSGFRVLLLFGSDELPSQDVTLDYDVRDTSGAAGNGTI
                     PVVSATREERPRVDFAMAIGSRTIRIAFDREVVRASLVADSITILPRVAITGVEAVDQ
                     FGRFFDIHFGSALSPTQVYVLRFAGVHGVAGEVFSTGVVVGGESVTEVPPRIIAVLQK
                     SATELWLVFSAQIDSAKADKADVRITPRVSVEKTLPGEITTVLIVKVLPETPIGPWQE
                     SYAVAARVDLADGSNPLLESVFFPSGERPSTGRLRAVEVSSSTRLRLRFDLPIASSGQ
                     PGAAVRVEPGIQVLSFSVADSIVDVEVSDQTPLGSWGIAYVVHVDGLLAASGERLDEL
                     FVVQMAPPSGLDSVRVFPQPFRPTHHHSLVFGGLPIGATLQIYRLDGSLIRDFAATET
                     GGITWDGKNGSGTAVSSGVYFYVVSSPLGRKTGKIAVVR"
     gene            11217..>11763
                     /locus_tag="PLT86_07765"
     CDS             11217..>11763
                     /locus_tag="PLT86_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT36546.1"
                     /translation="MKWAANNRYIVAFATLTLLGFAIGVRAGTTGKISGTVKSSDGTP
                     LPGVAIEVVGLRLGAMTDADGRYVILAVPPGQHELRASMVGFQAAIMQNVRVSADRTT
                     TASFTLREETIEVAPLVVTAGRPSIETDVTSSQTIVDARRVSEIPVGKMLDALGYEPG
                     VSVARDNELSIRGGGPSEIRFQ"
BASE COUNT         2315 a         3652 c         3436 g         2360 t
ORIGIN      
        1 tgcaggcaag cgtgatcggg ctcttgttct gttccgggag tttctcgccg aagcatcggt
       61 ggataacccc aaccgtgtga ttgcgcgggg gaaactgaag atggcgggtc aagacggagg
      121 tgaaccggag cctcgaatca cgaggcccgc gagtcctccg ctcttgactg cccgcccgaa
      181 cccctttaac ccgacgacat ccatcacgta ccgggtcacg gcgcagggga acgtgtcggt
      241 tcaggtgtac aacgcgcttg gccagcgcgt gcgcgaactg gtcaacacaa ccgcgcagcc
      301 gggggtctac actgtcgtgt gggacggcaa agacagcgcg gcaaggcctg ttgggagcgg
      361 tgtgtatctc gttcgcctca ccacgccgac ggcggtggta acggagcggg tgacgctggc
      421 tcgttgacag gtgcgttttc aggtaggggc aattcatgaa ttgcccctac gggtcccgca
      481 tttataggcc cggcggcggt ttaagcatcg tgtggcaacg tggcagccca cgtgttcatc
      541 accggcccga ccctatttgg caaactgccg cttctgccag tcgttggcga acagattgaa
      601 gaacccttcc ttgtacggat gctccttgat caggtcctcg ttgaggctga tgccgagccc
      661 cggcccggtc ggcagttcga cgtatccatc caccacgttc ggcacccccg gcacggcctc
      721 tttcacccac ggctccacaa aatcgtcaaa aatctcctgc accttgtagt tcgtggtgca
      781 gaacccgaag tggatcgacg ccgccgtgca caccggcccg ttggagttgt ggggcgccat
      841 gacgcggtac tgtgcgtccg ccatggcagc gatcttcttc atctcgagga agccacccgc
      901 gtggatgatg tccgcctgca cgatatccac tgcgcccgag ttgaggaact cggggaactg
      961 gtagcgcgtg aaccagcgtt cgcccgacgc gatggggatg ttcacctgct cgtgcacctg
     1021 tttcagggcc tgcgcgttct ccggcggtac cggctcctca taccacccgg ggttgaattc
     1081 ctcaagctgc ttgccgatct ggatcgcggt gcgcggtgag aagcgcccgt gcccctcgat
     1141 gaagatttcc acgtcgggac cgacggcgtc acggatggcg gcgacaagct ccagcgattt
     1201 catgatctcc tgccggctca tttcgtaggt gccggggccg aatgggtcca ctttgagcgc
     1261 cttgtagccc ttcgccgtca ccattttggc gcgcgccgcg aactgctgag gctcccgttc
     1321 cacggtgtac cacccgtttg cgtacgcctt gagcttctcg tgcgcctgcc caccgagaag
     1381 ccggtagacg ggctgccccg tcgctttgcc gatgatgtcc cagcaggcga tctcgaccgc
     1441 gctcatcacc gtcgtggcaa tgacaccgtt gcgccagaaa tcgttgcggt acatgcgtag
     1501 ccagaggtct tcgacattga aggggtcgct gccaatgacg tgccgccgct tggcaccttc
     1561 gaggtagccc agcacgccct cttcgcgatt ctgaatggtg gcttcgccga tgccggtgag
     1621 cccctcgtcc gtttcgactt tgatgaaaat gatgttccgc cagctcgtgc ccagtacgag
     1681 cggcttgacg tcagtgatgc gcatgggtga gatctccccg gtttgtcgtt gatcagagat
     1741 gcatcaagaa tcatacgcgc cgtgctttcc tcgcacaagc cagccgctta cagccacggc
     1801 tttttcggtg gcatggatgt tgcgtccggg tattgctggt gtgccccgtc ctgaagcggc
     1861 tgacgttcag cgggacatgt tcactccggg gctgttttct tgcctcaggg ttcaacagga
     1921 aactctttca aaggcacccg tccatgtctc cggaaatccc tcccgcgagc cttctcagtc
     1981 gcgcacaaaa gagattattc ggacctccca ggcacatcaa agacccgtcg ctgttgcaca
     2041 agatgtcgct gattcccctc ctcgcgtggg tcgggcttgg ggcggacggt ctgtcgtcat
     2101 cggcatacgg gcccgaagaa gccttccgtg cactgggaga acacgcgtat ctggcgcttt
     2161 ttatcggtct ggctactgcg ttgaccgtgt tcatcatctc cttcgcgtat tcccgcatca
     2221 tcgagcactt tccgcacggt ggaggtggat atgtcgtcgc cactcagatg ctcggcaaaa
     2281 acgcgggagt gatttcggga agtgccctgc tcgtggacta cgtcctgacc gtgactgtgt
     2341 ccatcgcgtc ctgcgtggat gcggttttca gctatctgcc tgctccggtg catgctcaca
     2401 aagtgcttgc ggcgtgcctg ttgcttctga tgatggtgat cctgaacatc cgcggggtga
     2461 aagaatcgat caccgttcta gcgccgatct tcctggtgtt catcgccacc catgcgctga
     2521 tgttgttgga cggcgttttc acgcacacgc agggtttcgc ccccatggtg gcggacgtca
     2581 agcggggaat cagcagagac gtgtccacaa tcgggtggat cggcgtcgca gcagtattcc
     2641 tgcgggcata ttccctcggt ggaggcacct acaccgggat cgaagctgtg tcgaacggcc
     2701 tgcagattct gcgcgatccc aaagtgaaga acgggaagcg cacgatgctc tatatggccc
     2761 tttctctcgc attcactgcg agtctgcttt tcctctgtta cctccttgtc tccgtgaaac
     2821 cggtcgaagg tcgcacactc aatgcagttc ttgccgacac gcttttcgcc gactggagat
     2881 tgggaggcac aatcgccttc gtcacgattt tttcggaggg agcactcctg ctcgtggccg
     2941 cgcaggctgg ctttgtggac ggcccccggg taatggcaaa catggcgctc gattcgtggt
     3001 ttccgcgccg gttcgcggcg ctttcgcaac ggttgacgat gcagaacggg atcgtcttga
     3061 tgggagtcgc cgcattcttt ctgctcctct atacgcacgg aagcgtttcc gcgcttatcg
     3121 tgatgtactc catcaacgtg ttcgtgacgt tctccctttc tcaactcggc atgtcgcgct
     3181 atttcatcaa tgagcggcgc acgatggcga gatgggtcaa caaactcagc gtgcacctcg
     3241 tcgcgctggt tctttgcgtc acgattctcg ccgtgatcgt gtttgagaaa ttcgccgaag
     3301 gcggatggtt gacggtgctc atcactgccg cagtcatcac ggcatgttac tggattcatg
     3361 cacactacga caaggtgaag aaaggagtcc gagaactcga cgagatgctc atggacctcc
     3421 cgctctcggg gcctgtctac acggcggccc ccaaacacaa cgaccagacc gcgattcagc
     3481 tcgtatcggg gtacaatggc ttcggcgtgc acactctgct caccattgcc cgaacgttcc
     3541 cgaatcttta caagaacttc gtctttgtct cgattgcgac cgtggattcg ggtttgttca
     3601 aagagtctga taccgtcgag gccttggagt cgaaggcgca ggccgatatg gagcgctacg
     3661 ttgagctcgc gcggaagctc gggttcgcgg cagaatggcg aaccgacgtg gccactgatg
     3721 tcgtcgaggg cgcggtgaag gtgtgcaggg aggtcgtcag agagtttccg cgctcaaccg
     3781 tctttgcagg tcaaatcacc ttccggctgg agaaaggcta tcacaggtta ctgcacaacg
     3841 aaaccagcta ttcgatacag cgccttttgc agtgggctgg tatcaccacg gtgatcctgc
     3901 cgatccgtat caagaactga ccgggaccgg tcaacctcgt tctgctggca ggaggaaacg
     3961 ccacgggcgc gtggctccgg ccaccgtccg tgaataggtc acgcaacaat acgcagatcc
     4021 gcgaggtata ttgcgcttgg cgggtcaccg gtgccgcttc cctcatggct ggaatagaac
     4081 gacagcaagc cgtcggaatc gcccagcgcc acgaaaccgg gataggacgt gtcgcccccg
     4141 ctcggcaact cggcgatttc ttccagcgtg tcgtcaacga gccaccagag agcggtgcgc
     4201 gacggcttct ccggctcact tttcctgccg ccgatgagat acccacccgc ccatttcgcg
     4261 agcatcggcc ctccgatctg gcgcgccagt ttgacccgcg accattcagt gaagggcggc
     4321 tgggaacggc acacgagtgc atttttcgct gcgccgtcac gtgcgatcgc caggagagag
     4381 ccgtcgggct cgaacaggaa cgccgtctcg tcgccatatt cgtgttggaa gaacccaacc
     4441 ggtttgtaaa cgaatccctc gtcgctctcc atcagggccg actccgtgat ccgcgttccg
     4501 tcgccgtggt ccgcggtgcg ggcaaatgcg cgctttcttc gcccacacag gtatgccttg
     4561 ccgccaaatg cggcagcccg ccagatgtag tgcccgtagg tgccctcaag gaatcgcggc
     4621 cctcgccaca cctcgccgtc gtcggaccac atacagtacc cgaggtgatg gttaatgtcg
     4681 cggttctcct cgcagagcca tgtccccgtg tacacaaaca gacgtgtgcc gaagacgagg
     4741 aaatgcggat cgcggacgtc ccgctcggga actccgaact cgcacacctg ctcccacgtc
     4801 actccgtcgg agcttcgcat cacccggatg cgcgaggtgg gaaataccat gtgtccatcc
     4861 gggcaggaac ggaatgtgag gtaaagctgg cctcggaact ggcaaaggtc ggtaaatgcg
     4921 ttgtgttctc cgttgttgaa cacgcaacgg acggattcga tatgaacgcg cactggtttc
     4981 cctccttcac ggtgcgatct gatcgcggta catctgccag aaaagaatcc acggatcaag
     5041 ggcaatgata tcgtcgtcct cgcggatttt gaacacgaag tggctgtgat ccacggcacc
     5101 ggacaacacc ccggcgcccc aagcgaactg ttcgcctttc ttcaacggcc ggtgttcggg
     5161 gacagtcagc tcggtcatgt gatgcgcctg cagtatccat tcggaaccgt cgggccatcg
     5221 gtggatgccg cgccaccggt tgttgttatg ccccatggcg aggctgttga aataggagtg
     5281 gtcgtggata tcgatcggcg cccagagcgg agttccgcgc gggtggttga tgtcgaggcc
     5341 gccgtgcgcc gaggcgccgt tgtacgggcc catccagcag tcttccccac ggtagcactg
     5401 ctcgatcttg aggccgcctt cgggcagcgg gcaccagggg tgaagcgaat ccgggcagat
     5461 gcggagggta gcatcctgaa acgcgaaacg cgcgtgtttc ttcgggcggc actccccgtg
     5521 ctcctccgtg aggaactcga aaatggcgtc caccgcgtcg aaccagattc gcacgccgtc
     5581 gatctcccac ggttcataga aactgcgttg actgggaatc tcacgttcga gcgcgtgctc
     5641 ccgtccattg atccgcagga cacaattaaa gcggtacagc gtctgtgcgg cgtcctcctc
     5701 cactttcagg tgcttcaacg ttgtccggac tacgtgcgca ctggtggaga caagtgcaat
     5761 gcgcaccacg tctcctgaga caagctggaa ttcgagcaca tcgccgtggt tcatctctac
     5821 tggtgtgaga gtgtcccgcg cgattcgtct ggtgattgcc cgttcagtcg atgtcgtgcc
     5881 ggtcatcgcg cctcccaaaa atccatgtgt gcgcgcggta tcagggtaac cccgcgctgc
     5941 acggggataa aaggccagga acgcatgtgg tgctgctccc gcctgcaggg aaatagccct
     6001 tcgtcctcgg acagaacgcc atgcaacaac cctgtatgtt tgttctcagc aggacggcgt
     6061 aacccggtcg aacgtaagcc ccgtggagct ccgtcattcc cgcgcaggcg ggaatccata
     6121 acaatccacc ccttggtgaa ttccgcccgt cgatcttccg ccgcctccaa aacagaactc
     6181 accgcaccac ggcgattttt ccggtcttcc taccaagagg cgaggatacg acgtagaaat
     6241 agacacctga cgagaccgca gtgccgctcc cgttcttccc gtcccacgtg atgccaccgg
     6301 tctccgtcgc agcgaaatct cggatcaggc tgccgtccag acggtagatc tgcagggttg
     6361 ccccgatggg gaggccgccg aagacgagtg aatggtgatg cgtcggcctg aacggctgcg
     6421 ggaagactcg gacgctgtcc agaccgctcg gaggagccat ttggacgaca aacagttcgt
     6481 ccagccgttc gcccgaagcc gcaaggagtc catccacgtg caccacgtac gcgatgcccc
     6541 aggaccccag gggcgtctgg tcgcttacct cgacgtcaac gatggaatcc gcgacgctga
     6601 acgacagcac ctggatgcca ggctcgacgc gaaccgccgc gccgggttga cccgaactcg
     6661 cgatcggcag atcgaacctc agcctgaggc gcgtggagct tgaaacctcc accgcgcgaa
     6721 ggcgacccgt ggagggtcgt tcccctgacg ggaagaagac ggattccaga agcggatttg
     6781 acccgtccgc aaggtccact ctggcagcaa ccgcgtacga ttcctgccag ggaccgatgg
     6841 gagtttccgg aaggaccttg acgatcagaa cggttgtgat ctcgccggga agggttttct
     6901 ccacggacac gcgcggggtg atccgcacat ctgccttgtc cgctttcgcg gaatcgattt
     6961 gcgccgaaaa caccaaccag agttccgtgg cgcttttctg aagcaccgcg ataatccgcg
     7021 gagggacctc ggttacgctt tcccctccca ctaccacacc ggtggagaaa acctctccgg
     7081 caactccgtg aacccccgcg aaacgaagca cgtagacctg tgtcggcgaa agtgccgaac
     7141 cgaaatggat gtcgaagaag cgtccgaact ggtcaacagc ttcgactccg gttatcgcca
     7201 cacgaggcag gattgtgatg gagtcggcga cgagcgatgc gcgcaccacc tcgcgatcga
     7261 acgcgatcct gatggtgcgg gaaccgattg ccatcgcaaa gtcaactcgg ggcctttcct
     7321 ctcgagtcgc agacacaacc ggaatcgtac cgttccccgc agcccctgag gtatctctca
     7381 cgtcatagtc cagcgtgacg tcctgtgaag gcaactcatc cgaaccgaac aggagcagca
     7441 cccggaaccc ggaccgatcc cacacgagcg aactcggcct gtgctcctgt gcaccggcgc
     7501 gaagcaggaa atctcgttcc gttgtctcca aggcgcttag cggaagatca aaccgcacat
     7561 ctaccccact gggagaaacc ctcgccgcgc aaagtgcttg cggccctgtg tgtggtcgaa
     7621 ccacaatcgc atcgctcacg ccaccaatgc tgtcagcgat ggcgggattc accgcttgca
     7681 cggcgacacg gatttcggat tgcaccggcg gggtgaacgt cagcaccacc ggagggacag
     7741 ggtctgcgaa ctcagtccga agtgaaaggg gcgcatctcg gccaacacct gtccatatcc
     7801 ggtagtgcaa gcccggttct gcgttccacg agacgactac cgagatcgaa tcccggaccc
     7861 ggactgacac gttgcgcggt gccgccggcg cacggggcga ggcgagagcg cgaagaaccg
     7921 ccaacgcgcc cccccgcggg aaaaggagct cagcacctcc ctgcccatca atgtcggcga
     7981 tgagaggctg gctcggagtg tccgtttcgt cgtaatacag gaacccgtca actcccggac
     8041 ccgtgggatc aagaacgtac aggtggggcg gcgttgccag aacaacgacg cgcctgccac
     8101 ccatatgccc ggaagaaaac ccgttcccct cggtgcgcgg atcgtgaaat tcgcgaacga
     8161 aaaatggagt cgtaccgccc gtacgcactt ccagtcgaaa ggctgccaca tcagcggtga
     8221 acttggtgtt ttccaggtgg cgcacgatcg cgaagtcgtc tcgcccgtcg tcgttaacgt
     8281 caccgattgc acacacgcgc gtcgcgtcgc cttgcccctc aatcactcgc ggaaccccaa
     8341 aggcatcgtc tccgacgctc gggaacagca ccaccgcacc accgacagtt cccgcaacga
     8401 ttgagttcct gcctgtgccg tcgaaatcgc ctgccgccct ccagataccg aactgcccct
     8461 cgtaaccgtt caccgggctc tgaaggatcg ctttgagttc gattctaccc ggcccggcag
     8521 cttcgtagac gtgcagcagc gcgcgattcg aatccaccac cgcgatgatt tccgcgcgcc
     8581 cgtcgccatc tacatctgcg acacttggtc ccaccagcga cgtccgctcc caccagggtg
     8641 acgactgacc gatctccacc acccgcaccc ggaacgctga atcgaagtag tcgaatccta
     8701 tcaggtccaa cttcgcgttc ccatcgatat caactactgc tgtcgggcgc atcttgacgg
     8761 gactctccgt ccgaaggacg ggaggagatc ctggaggaag ctcgaagaac gacaccgtgt
     8821 catacggcgc tcggtgcgca agtttgatga cagcttcagg aatcccgttc ccatcaatgt
     8881 cgggcaaggg gggcattccg gtgccggcag gcatcaggat tccaccggca cgcacatcct
     8941 cctcgtccca gcccgaccgg ggtattgtgt acgcgcttgc cgtgcccgtc gagaacgcgc
     9001 tctccgtttc catcccggaa gcatttctcg cgaccacctg aactgtaaag ggccccacac
     9061 cgacgtcttg cctgggtatt tcttcgaaat gggcccggtc ggtcgcagat acggaatact
     9121 ccaggggcga agatccgcca accgctccgc gccagatgcg caccgtgccg gttgtcagat
     9181 cgtcagtctc ccattgaaga aagattctcc gggtatcgcc atcccatcgc gggagaaccg
     9241 ccggctgtga ccggaacacg ggaggcgaac gatccaatga gtaaagcgtg cgatcttcgc
     9301 ctgttcgccc cagcaggtca gtcaccgtca ggcgcaggca gaaaagtgta tcaggatgat
     9361 cgatcgtgga gtacgtaccg cggagagtat ctcctgccgt ttccagaacg ccggacgcac
     9421 acgttgtcca cacccgtgga accgcgccga caccatagct tagaacccac gcgaccggcg
     9481 gagttccccg gacatgtacc gcccagcgga cggcatttcg aacctctttc gttcgtccgg
     9541 gggtcagtat ctgcgccacc gggtcctgtg ccaccgacaa aagcgttggc aggtccagcc
     9601 ttccatggcc agtttgctca tcccaacccg gcggcccgat gtcaaccacg ccccggcaaa
     9661 gaatggaacg cacttccgtg ggtgaaaggt gtggcgcgcg gctgaggagg atcgccgccg
     9721 cgcccgagac gaatggcgcg gaaaaggacg tgccaccgtt tcggcggtac gctccccagg
     9781 tgttcgtggt gtagatgctg acgcctggcg cgcagagatc gagattctcg ccataactgg
     9841 aaaacgtggc ccgcgcatca ttttcgcccg tggcgcctac cgcgatggtt tcgtcgtatg
     9901 cagcgggaaa gaggagcgaa ggtgtgttct catttcccgc agccgcaacc aagacaaccc
     9961 gcgcagccgt tgcgtacgcc acgatgtccg ccatgagagg cgtagacaca acgtcgccaa
    10021 aactcatgtt gatgatatct gcgccgtttt ccaccgcgta gacgaccgcg gccgcgatgt
    10081 cgtcttcctc aagatatccg ctcccgttga tgagttgcgc tcccgctctg aggcacatga
    10141 ttctggcgtt gggggcagtt cctgcaatac ccaccccgtt gttgacgaca gccgccgcaa
    10201 ctcccgcaac ctgcgttccg tgtcccattt catcgaacgg gtcgttatca cggtccagat
    10261 aatctccaag gccggacaat ccgggagagt ccgtgaaatc ccatcctacg aaatcgtcga
    10321 cgtacccgtt tccgtcgtca tctacccccg gaagccccgc ctgttcaatt gcgttgtgcc
    10381 agatgttggc ttccagatca ggatgccggt agtccacccc gctgtcgatg atcccgatga
    10441 tcgggcattt tgcggcgtcg gcttgaacct gttgccacac ctcatccaga cgaatgcgtt
    10501 gagcacccca ctgattctgc caacccggat cattcggcgc tgttccgagc attcggtaga
    10561 gataattcgg ctgcgccact acgccgggta tccggttcaa tgaatcggcg acctgctgcg
    10621 gggagagttc gttcgaaatc cggtacctga accaggcgcc cacctgttcg gggagtgcac
    10681 tcgcggatgg tttccgggaa cgaagaggga aaagcggttc aggatcggca agcccgagtt
    10741 ccgtcccgcg gaaaccgggt ttggccaggt ctccggtcat ccgcacaaga accgtgtgcg
    10801 gcgaaaaccg gggtgtttcg aggcagaatg cagaccccgc cgacaacgaa agcgccgcga
    10861 acaccgcaag caataatcca gcaccacctc ggagtcttcc gcttgtcaca gagcgtgaga
    10921 cgcggctgcc aactcgagtc gacaattcgt acacctcatg ggcagttcaa taatggcgtg
    10981 tcaaacggat tctgcgggtg acggtcgatg agttgcatat tacctgattc gccggtagct
    11041 tcttttcatg taacgatccc atgacggtgc cttttgacga acaacctgcc ttcccggggc
    11101 tgtgtcatcc ggagtgggaa ccgcttcgat acccggcacg gcatgtagca aaaatactca
    11161 aatcggtgat agaggccaca gcgccgcgaa ctctcgttct cgccgaggag aacacgatga
    11221 agtgggctgc caacaatcgg tacattgttg cgttcgcaac cctgacgttg ctgggatttg
    11281 ccatcggtgt ccgggccggc accacaggca agatatcggg gacggtgaag agcagtgacg
    11341 ggaccccgct tccgggtgta gcaatcgagg tggtgggatt gcgtctcggc gcaatgaccg
    11401 atgcagacgg tcgatacgtg atcctggcag tccctcccgg gcaacacgaa ctccgcgctt
    11461 caatggttgg cttccaggca gcaataatgc agaacgtccg agtgagcgcc gatagaacga
    11521 caactgcgag tttcactctt cgcgaggaaa ctatcgaggt tgctcctctg gtcgtcactg
    11581 caggccggcc gagcatagaa acagatgtga cgtcctccca gacaattgtt gacgcacgga
    11641 gggtatcgga gatcccggta gggaagatgc tggatgcatt gggctatgag cccggtgtct
    11701 ccgtcgctcg ggacaacgaa ctctccatcc ggggcggagg cccctctgag atcaggtttc
    11761 agg
//
LOCUS       DAOSOO010000088        11731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5440_length_11731_cov_6.199897, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000088 DAOSOO010000000
VERSION     DAOSOO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_11731_cov_6.199897"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2871)
                     /gene="sprA"
                     /locus_tag="PLT86_07770"
     CDS             complement(<1..2871)
                     /gene="sprA"
                     /locus_tag="PLT86_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOT36547.1"
                     /translation="MPGKWCAASRFGALSVGLWLFSSFGTPSAQHLPPVAPAAFDSAG
                     TDTSSAVPDSGRVQLPRQRFSPYKPPQPGFFYTRKSQIDFSRGTVTTITSVGGVEIGE
                     RVVRPLDKAIAEDIAPRLAASLSQTRRQQGRPTTGGDTGPRIEMQYEIPNLPPVAKTI
                     IGEGPSTLHISGYGRITMSGRSQYRTGETVGSTYQQSKFPTLSMQQELSFKIDGTIGS
                     KIYVSVDQDTRRISELDNNVHIRYQGEEDEIVQEVEVGNTNLSLPGTQFVSGGVQHSG
                     LFGIKARAQFGDLDVTMIASQQKSSSQKRTFRGGAQASETKILDAEYIPRRFYFLDAS
                     YRIQYAAALEKLRNNPSMKDLDIGIVPGSGQRPWAREIVEVNLYRGVAATAGQVDVKD
                     GFGVPAMLTDSSGNDLTLSPYDPATLQAMAEGSGGRIRQGGWQLIPSTEYYLDPDLGY
                     VDFGTSLSDGAIIGVVIKFINQAGVIDSFPKCTDPSVLVTKMVKYAPSRPTDPTWTYE
                     WRHVYFVGARDLSPTDFRLEIVDAKVAGTPNTPANSAQTYLQLMQLDVASGTDLTGPA
                     DGLVDNNRVKLDLKTGLVYFPFLEPFQEAFVSLEGEGNPALYQETNLSGRDKYALVAT
                     YSKASTRIQLGFNIMENTEVVKLNGVRLTRNIDYTINYFTGELLFTQSVADQVSQPGA
                     DLSIDYEMNPIFKPDQESLVGLSGVYRLGERGNLGGIVMYNSERTSSTRVRVGEEPSR
                     MTVFATYANYEFRPSWITTAVDMLPFIESDEPSTLRLEGEIAQSLPNLNTRGTAYLDD
                     FEGAGNRTPVGIGRRGWALASPPVDLRLRPNELDRNRRGKFVWFNPYSRVKTIDVFTS
                     LSENELTQSERFMDVLNLWFRPKGTTDSERQASWGGIMRSFGTTGVDLQKTQFLEIWV
                     RAGANPFDPDRIGGVARPTGTPVLHIDLGDMSENSHLSTRTGI"
     gene            3284..4642
                     /locus_tag="PLT86_07775"
     CDS             3284..4642
                     /locus_tag="PLT86_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT36548.1"
                     /translation="MRTIWRAVLREHIGPFFFAFAVVFFILVLDLLIDVMDSILGKGI
                     AVGTVLQLFVLNFAWIIALAVPMAVLVSVLMAFARLANDNEITALMAVGISPASLVVP
                     SVVAGGIVAAGLMWFNNAVLPESNFRARVLLNALREKKPAISLKSREGQFITDFPGYV
                     LRVDRVILESPDAGDDASGSRLEGILIYQFDQAGGTRPTVVTAKSGTIEMWDGGATVR
                     LVLNDGEMSQVDSKNPESDIRTQFQRQTIIIADRQRGNPEQLRRAEGFRGDRELSSAA
                     MLERARMYDRQVEAVDARIAALVADTGMAADYRRQQIASEERVRQGYRQMANQYRVEI
                     HKKFSIPVACIVFVLIGAPLGMMARGTGRSVAIVASLVLFLLYWASLIAGEQLADRGY
                     LPPWLAMWGANIIVGLWGIALLFSVTWNVRMASLMELVPRAIEARRSRKASSSAAKGF
                     VD"
     gene            4762..5772
                     /locus_tag="PLT86_07780"
     CDS             4762..5772
                     /locus_tag="PLT86_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOT36549.1"
                     /translation="MPARRRSSFGFLWIAAVLAASGSSCGVLATQPYRSLAPVTPVQD
                     RDQAVQIIVAALTERGYSPALVNERVGTVSTDWRMTAGWMKIFFGYSGQDRVVVSLSD
                     STLILNGEYQMRKGTSLVGAMFHDRDDRRRDESDTDWFTTNPPASLRQEWEQLRLIIA
                     ERISQAAAARRATATGAASVVAAPPESAVAPPVLVRRQPAEQRVAVAVVDFKGVGVSR
                     DEVAILTDRLRAELVDTGIFMVIERERMEEILREQEFQISGACESDACVVEIGRLVGV
                     NRIVTGTIGKLGSTYTTTARLINVETGEIVARASDDCACEVEQLLGRMERIAGVLANG
                     AN"
     gene            5803..7068
                     /locus_tag="PLT86_07785"
     CDS             5803..7068
                     /locus_tag="PLT86_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36550.1"
                     /translation="MERKTVAVPLRGIVLPSLDRGAAERVAKWVGEWHSADYDPEEIS
                     STLANLPPEVPVDDLLEPFDIVAEEESAQALAELALALIHYVERGCLQAAEARDVFRM
                     ACLQLCNRGIPFEETAKGDDLAQWFHYIGVNEFGDTDDSGFSTDLTDLRLLSRYFLDP
                     GRLAEELFDKPHAEFPTNYHEGYIGIAGALGDWRAAPFLLRILSRCEDSPSVATTIAA
                     LGMLGRARKGWSAAYRDRVAQVLKGYAFSDAPEVATSAVLALEGLGGIHAEATLRELE
                     RVLPAGRDMLSAHVSHALIHLEKGDEALEKELARIASDPKEPQITRICAIERMAGSDE
                     SETICTLADLLDDPAVEPVRVDGYLTDDVVHVIREAAFMSIMDCRFRRLAEVLGEPVL
                     DRIEKFQTSYPPSWWIRARIGNREFEEDE"
     gene            7177..8913
                     /locus_tag="PLT86_07790"
     CDS             7177..8913
                     /locus_tag="PLT86_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT36551.1"
                     /translation="MIRRIAALATGACAVFCCAKVCPADAVQTDSVRVVVRGARAFPA
                     DRIRLWASGPDGLRRIGKEYSDAGFWSAILQDTLYVSRNGDAVREILVHEGDIASVTL
                     AIQRTRGDVTDSLSERLPGFRSTPDAVYRLLRREAEEGYPFAEATLVSAHEHTASRFV
                     VDTRLVSGPLVRVARVVPRGNTITKSHVIARELRRTPGSAYSQKDVERWVRRLNRSGM
                     FDAVGEPALAWSDSASGTADLLISVREGRPNRFEGVVGYQPGEGNSRGEFTGLADVTL
                     GNLLGTGRRLAVRWNRPQAEQTTLDLSYREPWVAGFPVDAEGKLAVEQRTAYALERLE
                     LAVGGELVPDVVVAASIGREIVRSDSVPLLGGPRYRSMTLRGEAEYDTRDVRENPTTG
                     MRYALNWRYDMRRNRVYDSDFLAYYGGSAWLRNERVSVVKVDLEHFLPVRRAIVAAVG
                     WHGGQISSTSGRTTFSAADELRLGGALTLRGYRQDQFAGDRIIWGNHELRYLLGGAAR
                     VFVFVDAGYVRTRRIDAQSGMTASAETYPVGYGGGLRARTTAGNLGIDFGWGRHDTFS
                     RGKVHARIETTF"
     gene            9092..10480
                     /locus_tag="PLT86_07795"
     CDS             9092..10480
                     /locus_tag="PLT86_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36552.1"
                     /translation="MPVPTNLPDNIRTVLNACPPLRNQRGSRELVYQYSIGSLSALND
                     EEAEWTIRELAARGIGVISMWQTGANQDAGIAEALRIGRIQKKLGLTVACDANALLYS
                     FYDKTAATAHVDEAGQPFFDESFSGPKMGCPFALEHRMPVILGRVAAYVEAYHQAGVP
                     IGIVTADWEIDGPHEWNDAWRSSRQCARCRENLPNIDDFSAFQARMRQMRSRLQRECY
                     ADPILARNPKALVTNYAVYPNDGWRYWYDYFEHPQPELPHKRDQNDLHRPWYNDFFET
                     HFTLAMPVVYTWYRIYDAYPEFTNPDYRWFYNMLLVGTNAGKSTPSGIPIATFVHWHT
                     TAPPADSPAVPQMSAEAYTELLWHLLLRGHDIFYSWTPMDELAEEIALVQRVYDGSLL
                     YNDWLLNGTPICFDVPPQQGPVISGVRVGNQVLLRRTDFGDVREPVTITVDGRRLVVP
                     VSPGRMQILDLE"
     gene            10485..>11731
                     /locus_tag="PLT86_07800"
     CDS             10485..>11731
                     /locus_tag="PLT86_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36553.1"
                     /translation="MNGVPIPDEPVRAALIGAGSRARSIYRPIMPSMSDWMRVVAVCD
                     PVREHADALAASYGVPAFYDIRELVRSRPMEAAVVVTPVESHHSISCFLSAHGIHNLV
                     ETSWCNTVTQGRQMIEAARRGKVLVRVAENFFRFPIDRIVGKLAAAEVVGPVHRVVSY
                     ADHTGYHNNSRWVAFFGAHPEWVQSVEHTMPTMAFHESPERFHDSETYRARFFQFPGN
                     GFVMDHLANVKGFLGRYPRPGYTEWQGERGTIVHFARRGWQGEGEIRLCTDSEFGRRG
                     ISDTTIPIVTECPDHRWTRTYADLRPVGGGVVEYVNPFRPEEIPQHASDWYGCAIMDH
                     LADFACAVRGVRPSEFDEQDALMSMMMEVGAKESALKEGARVRLPIQGEPESDGRIHA
                     ALRARFGVDPLDIEAMLAISYPKP"
BASE COUNT         2157 a         3492 c         3725 g         2357 t
ORIGIN      
        1 gattccggtt ctggtcgaca ggtgggaatt ctctgacata tccccgaggt cgatatgcag
       61 tacgggagtt ccggttggcc gcgcaacccc tccgatacgg tcagggtcaa acggatttgc
      121 acctgcgcgg acccatatct ccaggaattg cgttttctgc aggtccaccc ccgttgttcc
      181 gaaggaacgc ataattcctc cccagcttgc ctggcgctcg gaatccgtcg tccctttggg
      241 ccggaaccag agattgagga catccatgaa gcgttccgac tgggtgagct cgttttcgga
      301 gaggctggtg aacacgtcta tcgtcttgac ccggctgtac gggttgaacc atacgaactt
      361 gccgcggcgg tttcggtcaa gttcattggg gcgcagccgt aaatccaccg gcgggctggc
      421 aagcgcccac cccctgcgcc cgataccgac gggagtccgg ttgccggccc cttcgaagtc
      481 gtccagataa gccgtaccgc gcgtgttgag gttcgggagg ctctgcgcga tttcgccttc
      541 gaggcgcagg gtggaaggct cgtcactttc gatgaacggg agcatgtcca ccgccgttgt
      601 gatccacgac ggccggaatt cataattcgc gtacgtggca aacacggtca tgcggctcgg
      661 ttcctcaccg actcgcacgc gcgtggacga ggttcgctca ctgttgtaca tcacgattcc
      721 gccgaggttg ccccgttcgc caagccggta cactccgctc agacctacaa gggactcctg
      781 gtcgggtttg aaaatcggat tcatctcgta gtcgatgctc aggtccgcgc cgggctggct
      841 tacctgatcc gccacggatt gcgtgaacag gagttctccg gtgaagtagt tgatggtgta
      901 atctatgttc cgcgtgaggc gcacgccgtt cagtttcacc acctccgtgt tttccatgat
      961 gttgaacccg agctggatcc gtgtggaggc tttgctgtac gtcgcgacca gcgcgtattt
     1021 gtcacgaccc gacaggttgg tttcctgata aagcgcagga ttcccttcac cttcaagaga
     1081 aacgaacgcc tcctggaacg gctccaggaa agggaagtac acaagaccgg tcttcaggtc
     1141 gagtttcacc cggttgttgt ccaccagacc gtccgcgggt cctgtcaaat cggttcccga
     1201 cgccacgtcc agttgcatca gttggagata cgtttgcgcc gaattggcgg gagtgttggg
     1261 cgtgcctgcc acttttgcgt caacgatttc caaccggaaa tctgtcggac tcaagtcacg
     1321 cgcaccgacg aaatagacgt gccgccattc gtacgtccat gtcgggtcgg tgggccggct
     1381 cggggcgtat ttcaccatct tggtaaccag cacgctcggg tcggtgcatt tggggaacga
     1441 atcaatcacc cccgcctggt tgatgaattt gatcaccacg ccgatgatcg ccccgtctga
     1501 aaggctcgtt ccgaaatcga cgtacccgag gtcagggtcg aggtagtatt cggtgctcgg
     1561 gatgagctgc cacccgccct gccgaatgcg gcccccgctt ccttccgcca tcgcctgcag
     1621 ggttgcgggg tcgtagggtg aaagcgtcag atcgttgccg gaagaatccg tgagcatggc
     1681 cggaacgccg aatccgtctt tgacgtccac ctgtccggcc gtggccgcaa cgccccggta
     1741 caggttcact tcgacgatct cccgcgccca cggccgttgc cctgagccgg gaacgattcc
     1801 gatgtcgagg tctttcatcg acgggttgtt gcgaagcttt tccagcgcgg cagcgtactg
     1861 aattcggtag ctcgcatcaa ggaagtagaa ccggcgaggg atgtattccg cgtccagaat
     1921 ctttgtttcg gaagcctgtg cgcctccgcg gaaggtgcgt ttctgtgatg agctcttttg
     1981 ctggctggcg atcattgtca cgtcgagatc gccgaactga gccctcgctt tgatgccgaa
     2041 cagaccgctg tgctgaacgc cgccgctgac aaactgcgtt ccgggcaggg agaggttagt
     2101 gttgccgacc tcaacctcct ggacaatctc gtcctcttcc ccctggtagc ggatatggac
     2161 gttgttgtcc agctcgctga tccggcgcgt atcctgatcc acgctgacgt agattttgct
     2221 tccaatggtc ccgtcaattt tgaaactgag ttcctgttgc atggaaagcg tggggaactt
     2281 gctctgctgg taggtggaac ctacggtttc cccggtgcgg tactggctcc tgccgctcat
     2341 cgtgattcgg ccgtaccccg atatgtggag tgtgctgggg ccttcgccaa tgattgtttt
     2401 cgcaaccggc ggcaggttag gaatctcata ctgcatctcg atgcgcgggc cggtatcgcc
     2461 gccggtggtg gggcgcccct gctggcggcg tgtctggctg aggctggccg ccaaccgggg
     2521 agcgatgtcc tctgcgattg ccttgtccag cggcctgacg acccgttctc cgatttccac
     2581 gccacccacg gaggtgattg tggttaccgt gccgcgggag aaatcaatct gggatttgcg
     2641 ggtgtagaag aaccccggct ggggtggctt gtacggcgag aaacgttgcc gcgggagctg
     2701 cacgcggccg gagtcgggaa cagccgacga ggtatccgta cccgccgaat cgaaggccgc
     2761 gggggcgact ggcggcaggt gttgcgcgga gggcgtgccg aaactcgaaa agagccacaa
     2821 tcccacggaa agggccccaa atcgagacgc ggcacaccac tttcccggca cgtgaggaag
     2881 ctccctctca aaccaccaga agggcggggc tgccaaccga aaccacgaga ctaccgacgt
     2941 atgggtttcc ccgggacccg cgcaaccgga tggcgggcca cccggaggca cggaaacggc
     3001 acacacggcc ccggagtttg cgtgcacgac gtggcatcgg taccttccgc cataccacgg
     3061 agtggcgccg cacgttcacc agagggacgt gtcgcgtgtt tcgcacgagg aacgaacgca
     3121 gtgggcgata ttcacggcct ttgcgagaag aagacacccc cggtgacgcc ggggaacccg
     3181 tcgcccccga ccgggctgga gcgaacgtag agtataccca cgcggaaacc ggctttacaa
     3241 ccccggcgat ctggtggccg gtacgaattc aggatgcgcg catatgcgaa cgatctggag
     3301 agccgtcctg cgggagcaca ttggtccgtt ctttttcgcg ttcgcggtgg tgttcttcat
     3361 ccttgtcctc gatttgctga tcgacgtcat ggacagcatt ctgggcaagg ggatcgccgt
     3421 cggaacggtt ctgcagcttt ttgttctcaa cttcgcgtgg atcattgcgc tcgcggtgcc
     3481 gatggcggtt cttgtttcgg tgctcatggc gtttgcgcgg ttggccaacg acaacgagat
     3541 cacggcgttg atggccgtcg gaatcagccc ggcctcactt gtggtcccgt ccgtggtggc
     3601 cggcggtatc gtagccgccg gtctcatgtg gttcaacaat gcggttcttc cggagagcaa
     3661 tttccgggca cgggttctcc tgaatgcctt gagagagaag aagccggcga tcagcctcaa
     3721 aagccgggaa gggcagttca tcactgattt ccccgggtac gtcctgcgcg tagatcgggt
     3781 gatactggaa tcaccggatg cgggggacga cgcctcgggg tctcgtctgg aaggcattct
     3841 gatctatcaa tttgaccagg cgggcgggac ccggccgacc gttgtcacgg cgaagagcgg
     3901 aacgattgaa atgtgggacg gtggcgcgac ggtgcgcctc gtcctgaacg acggggaaat
     3961 gagtcaggtt gactcgaaaa acccggaaag tgacatacgc acgcagtttc aacggcagac
     4021 aatcattatt gccgaccgac agagggggaa ccctgagcag cttcggcggg cggaggggtt
     4081 ccgcggtgat cgcgagctct cctccgcggc catgttggag cgtgcacgca tgtacgatcg
     4141 ccaggtcgaa gcggtcgacg cacggatcgc cgcgctggtt gccgacacgg ggatggcggc
     4201 ggattaccgc aggcagcaga ttgcgtcaga agaaagggtg cgccagggct accggcagat
     4261 ggcaaatcag taccgcgtgg aaatccacaa aaagttctcg attcctgtcg cctgcatcgt
     4321 attcgtcctc atcggggctc cgctcggcat gatggcacgc gggaccggtc gctccgtggc
     4381 aatcgttgcc agtctcgtgt tgttccttct ctactgggca tctctcattg ccggtgaaca
     4441 acttgccgac agaggatacc tcccgccctg gctggcgatg tggggggcca acatcatcgt
     4501 cgggctgtgg gggatcgccc tgttgttcag cgtaacgtgg aacgtccgga tggcgagtct
     4561 catggagctt gtgccgcgtg cgattgaggc gcggagatcg cgaaaggcat cgtcgtcggc
     4621 agcgaagggc ttcgttgatt gacaaatcgc cccccgcggg gtacccttaa tacgtcacca
     4681 atgtgacacg cctcacagtc cgtagcagac agtccgtgcc ggacagtccg tctttaccat
     4741 tcggaacgag tttatccagc cttgcctgca cgtcgccgtt ccagcttcgg attcctctgg
     4801 atcgccgcgg tgcttgccgc gtccggatcg agctgcggcg ttctggccac ccagccgtac
     4861 cgttcgctgg ctcccgtcac gcctgttcaa gaccgggacc aggccgttca gattatcgtc
     4921 gccgccctga ctgagcgagg atacagcccc gccctcgtga acgaacgcgt gggtactgtt
     4981 tccactgatt ggcgcatgac ggcaggttgg atgaagatct tcttcggcta ttcggggcag
     5041 gatcgtgtgg tcgtgtcgct ttccgacagc acgctgattc tcaacggcga gtatcagatg
     5101 cgaaaaggca cctcgcttgt gggcgccatg ttccatgaca gggacgacag gcgccgggat
     5161 gagtcggaca ctgactggtt caccacgaac ccacccgcct cattgcgtca ggagtgggaa
     5221 caactgcggc tcatcatcgc ggagcggatc tcacaggcgg ctgcagcgcg ccgcgcgacg
     5281 gccaccggtg cggcttccgt ggttgcggcg ccccccgaat cggctgtggc gccgcccgtg
     5341 ttggttcgtc gtcagccggc agagcaacgg gtagcggtgg ccgtggtgga tttcaaaggg
     5401 gtaggggtat caagggacga agtggccatt cttaccgacc gtttgcgggc tgagctcgtg
     5461 gacactggta tatttatggt gatagaacgt gaacgcatgg aagagattct gcgcgaacag
     5521 gagttccaga tttccggtgc gtgtgaatct gacgcctgcg tggtggaaat cggtcgcctc
     5581 gtcggtgtca accgtatcgt tacgggaacg atcggcaaac tcggaagtac gtacaccacc
     5641 acggcacgcc tcatcaacgt ggaaaccggg gaaatcgtcg cccgggcgtc ggatgattgc
     5701 gcgtgcgaag tggagcaact gctcggacgg atggaacgga tcgctggcgt tcttgccaat
     5761 ggcgcaaact gatccacccc atttcccctg ccggagtcgc acatggagcg aaaaaccgta
     5821 gcggttcccc ttcgcgggat cgtcctgccg tctcttgacc ggggcgctgc cgaacgtgtg
     5881 gcgaaatggg tcggcgaatg gcacagtgcc gactatgatc ccgaggagat ttcgtccacc
     5941 ctcgcgaacc tgcctcccga ggttcccgtg gacgaccttc tggagccgtt cgacattgtt
     6001 gcggaggagg aaagcgcgca ggcgctggcg gaactggcgc tcgccctgat ccattacgtt
     6061 gagcgcgggt gcctccaggc ggccgaggcg cgtgatgtgt tccgtatggc gtgcctgcaa
     6121 ctgtgcaatc gcggcattcc gttcgaagaa accgccaaag gggacgacct cgcgcagtgg
     6181 tttcattata tcggcgtcaa tgagttcggc gataccgacg attcggggtt ctccaccgat
     6241 ctgactgacc tgcgactgct gtcgcgctat ttcctcgatc ccgggcggct ggcggaagaa
     6301 ctttttgaca aaccccatgc ggaattcccc acaaattatc atgaaggtta catcggcatc
     6361 gcaggcgcac tgggagactg gagagctgcg ccctttctcc ttcgcatcct gagccgttgt
     6421 gaagattccc ccagcgtcgc caccacgatt gctgccctgg ggatgctcgg ccgcgcccga
     6481 aaggggtggt cggcggcgta tcgcgataga gtcgcccagg tgctcaaagg gtacgccttt
     6541 tccgacgctc ctgaggtggc aacttccgcg gtgctggctc tggagggtct tggcggtatt
     6601 cacgcagaag caacgctgcg cgaactcgaa cgtgtgcttc ccgccgggcg cgacatgctt
     6661 tccgcgcacg tcagccacgc gctgatccac cttgaaaaag gggatgaggc gctggaaaaa
     6721 gaacttgcgc gcatcgcttc cgacccgaaa gaaccacaga tcacccgcat atgcgccatc
     6781 gaacgcatgg cgggaagcga cgaatcagaa accatctgta ccctcgctga cctgctggac
     6841 gaccccgcag tggaaccggt ccgcgtggac gggtatctca cggacgacgt cgtgcatgtg
     6901 attcgggaag cggccttcat gtccatcatg gattgccggt tccgtcgtct tgcggaagtg
     6961 ctcggagaac cggttctcga tcgtatagag aagttccaga cctcctatcc gccgtcgtgg
     7021 tggattcgag cccgaatcgg caaccgggag ttcgaggaag acgagtagtc ctccggttgg
     7081 ggttcgcccg atgtccccct ccctccattc ggcccggcgt gctcctgcgg gttccggagc
     7141 gtttcccaca tccggcagac cccggaaccg ctgcccatga tacgcagaat cgctgccctt
     7201 gcgaccggcg cgtgcgccgt gttctgctgc gccaaagtct gccccgccga tgccgtccag
     7261 actgactcgg ttcgcgtggt tgtccgcggc gcgagggcgt ttccggcgga tcgcatccgt
     7321 ctgtgggcgt ccggccctga cgggttgcgc cgcatcggga aggaatactc agatgcaggc
     7381 ttctggtccg ccattctgca ggacacgtta tacgtctccc gaaacgggga tgccgtgcgc
     7441 gaaatactgg ttcacgaggg cgatatcgcc agcgtcaccc ttgcaatcca gcgcacgcgg
     7501 ggggacgtga cggattcact ttcggagcgc ctgccgggtt ttcgatccac acccgacgca
     7561 gtgtaccggc tccttcgacg ggaagcagag gagggatacc cgtttgccga agcgacgctt
     7621 gtatcagcgc acgagcacac cgcttcgcga ttcgtggtgg atacgcgcct ggtgtccggc
     7681 cctctcgtgc gtgtcgcacg cgtggttccc cggggcaaca caatcacgaa aagtcacgtc
     7741 attgcgcgcg aactgcgccg gacgccggga agcgcgtatt cccagaaaga cgttgagcgc
     7801 tgggtacggc ggttgaatcg ttcggggatg ttcgatgccg taggggagcc tgccctggca
     7861 tggagcgact ccgcgtccgg aaccgctgat cttctcatat ccgttcggga aggccgcccg
     7921 aacaggtttg agggtgtcgt cggttaccag ccgggcgagg gcaactcgcg cggagagttc
     7981 acaggtcttg cggatgtgac gctgggcaac ctgctgggta cgggacgtcg gcttgcggtt
     8041 cgctggaatc gtcctcaggc agaacagacc acgctggacc tttcgtaccg ggagccgtgg
     8101 gtcgccggct ttcctgtgga tgcggaaggg aagcttgccg ttgagcagcg caccgcgtac
     8161 gcgctcgagc gtcttgaact ggcggttggc ggagaactcg tccccgacgt ggtggtggcg
     8221 gcaagcatcg gccgcgaaat cgtccgtagc gactcagtac cccttctcgg tggtccgcgg
     8281 taccggagca tgacgttgcg aggggaagcg gaatacgaca cgcgggacgt tcgggagaac
     8341 cccacgaccg gcatgcgata cgcgttgaac tggcggtacg atatgcggag gaatcgggtg
     8401 tacgactccg atttcctcgc gtactacggc ggatccgcct ggttacggaa cgagcgagtg
     8461 tccgtggtga aggttgacct tgagcatttc ctgccggtgc ggcgcgccat tgtcgccgcg
     8521 gttggctggc acggagggca gatttccagc accagcgggc ggaccacatt ctccgccgcg
     8581 gacgaactgc gcctgggagg tgctctgaca ttgcgcgggt accgacagga ccagttcgcc
     8641 ggtgatcgga tcatctgggg caatcacgag ttacggtacc ttcttggagg ggccgcgcgc
     8701 gtgttcgtgt tcgttgatgc cgggtacgtg cgcacacgcc ggatagacgc gcagagcggc
     8761 atgaccgcat cggcagagac ataccctgtc ggatacgggg gagggcttcg cgcccgcacc
     8821 accgcaggga atctcggaat tgatttcgga tgggggcggc acgacacatt ctcacgggga
     8881 aaagtgcacg cccggatcga gactacgttc tgaccgattc atgcgtcgat cagtttccgt
     8941 tgcgcatttc cgacgtcttc cagtatcatg gatacgtgtt cccgcatgtg gcgcagattg
     9001 cccgcgtgtt tctgaagggt cgataatctt gttccatctg acaggtgttt tgttggcgtt
     9061 cgcctccacc gcgttttcaa gggagttacc catgcctgtt cccacgaatc ttccggacaa
     9121 catccgcacc gtcctcaacg cctgccctcc gctgaggaac cagaggggct cgcgggagct
     9181 tgtgtatcag tattccatcg gcagcctgag cgcgttgaac gacgaagaag ccgaatggac
     9241 gatccgtgaa cttgcagcgc ggggaatcgg cgtcatcagc atgtggcaga ccggggcgaa
     9301 ccaggatgcc ggaatcgcag aagcgttgcg gatcgggagg atacagaaaa aactcgggct
     9361 tacggtggcc tgcgatgcaa acgctctgct gtacagcttt tacgacaaaa cagccgccac
     9421 cgcgcacgtg gacgaagcgg gacagccgtt cttcgatgaa tcgttcagcg ggccgaaaat
     9481 ggggtgtccc ttcgcactgg aacaccgaat gcccgtcata ctgggtcgcg ttgcggcgta
     9541 cgtggaggca taccatcaag ccggcgttcc catcgggatc gtgactgcag actgggagat
     9601 tgatggcccg cacgagtgga acgatgcgtg gaggtcttcc agacagtgcg cgcggtgccg
     9661 cgagaacctg ccgaacatag acgatttttc cgcgtttcag gcgcgcatgc gccagatgcg
     9721 cagcaggctc cagcgagagt gttacgcgga ccctatcctt gcgcgaaacc cgaaggcgct
     9781 tgtgacgaat tacgcggtgt acccaaacga cgggtggcgg tactggtacg actatttcga
     9841 gcatccacag ccggagcttc cccacaaaag agaccagaac gaccttcacc gtccgtggta
     9901 caacgatttc ttcgagacgc atttcactct tgcgatgccg gtcgtgtata cctggtaccg
     9961 catttatgac gcgtacccgg agttcaccaa tcccgattac cgctggttct acaatatgct
    10021 tctggtaggc acgaacgcag gcaaatccac cccgtcaggc attccgatcg ccacgttcgt
    10081 tcactggcat accacagctc cgccggcaga cagcccggct gtgccgcaga tgagcgcgga
    10141 agcgtatacg gaactgctct ggcacctcct gctgcgggga cacgacatct tctactcatg
    10201 gacgccgatg gacgagctgg cagaggaaat cgcccttgtc cagcgcgtgt acgacggctc
    10261 cctgctctac aatgactggc tgctcaacgg caccccgatc tgcttcgatg ttccaccgca
    10321 gcagggaccg gtcatttcgg gagttcgtgt ggggaaccag gtgctcctgc ggagaacgga
    10381 tttcggtgat gtgcgggaac ccgtgacgat caccgtggat ggccgcagac tcgtcgttcc
    10441 tgtctcgccg ggtcgcatgc agatcctcga cctcgagtag acgcgtgaac ggcgttccca
    10501 ttcccgacga accggtccgc gctgcgctga tcggcgcggg ctcgcgagca cgctcaattt
    10561 accgcccgat catgccgtcc atgagcgact ggatgcgcgt cgtggcggtg tgcgacccgg
    10621 tgcgtgagca cgcggatgcg ctcgccgcgt cgtacggtgt gccggcgttc tacgacatcc
    10681 gcgagctggt gcgttcacgc ccgatggaag cggcagtggt ggttactccg gtggagtcgc
    10741 accattccat ctcctgcttc ctttccgcgc acggcattca caacctcgtg gaaacaagct
    10801 ggtgtaacac cgtcacgcag gggcggcaga tgatcgaagc ggcgcgccgg ggcaaggtgc
    10861 tcgtgcgcgt ggccgagaac ttcttccgct tcccgataga ccgcatcgtc gggaagctcg
    10921 ccgccgcgga agtggtcggc ccggtgcacc gggtggtttc ctacgccgac cacaccggtt
    10981 accacaacaa ctcgcgctgg gtcgccttct tcggggccca tcccgagtgg gtgcaatcgg
    11041 tcgagcacac catgcccacc atggcgtttc acgaatcgcc ggagcggttc cacgacagcg
    11101 aaacctatcg ggcgcgtttt ttccagttcc cgggcaacgg cttcgtcatg gatcacctgg
    11161 ccaacgtgaa gggctttctc ggacgctatc cgcgccccgg ctacaccgaa tggcagggcg
    11221 agcgcggcac catcgtccat ttcgcgcggc ggggctggca gggagagggg gagatccggc
    11281 tctgcacgga ttccgagttt ggccggcgcg gcatcagcga caccacgatc ccgattgtca
    11341 ccgagtgccc ggaccaccga tggacacgca catacgcgga cctgagaccc gtcggcggcg
    11401 gcgtggtgga atacgttaac cccttccgcc cggaggagat accgcagcac gccagcgact
    11461 ggtacggctg cgccatcatg gaccatctcg ccgatttcgc ctgcgcggtg cggggagtcc
    11521 ggccaagcga attcgacgag caggacgcgc tcatgtccat gatgatggaa gtaggcgcga
    11581 aggaatcggc gctgaaagaa ggcgctcgcg tgcgcctgcc gattcagggg gaaccggaga
    11641 gcgacgggcg gattcatgcg gcgctgcgcg cacggttcgg cgttgatccg cttgacatcg
    11701 aggccatgct ggcgatcagc tacccgaagc c
//
LOCUS       DAOSOO010000089        11718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5448_length_11718_cov_5.578753, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000089 DAOSOO010000000
VERSION     DAOSOO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5448_length_11718_cov_5.578753"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLT86_07805"
     CDS             <1..1184
                     /locus_tag="PLT86_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT36554.1"
                     /translation="PFVMLQHVRRVIGDALATQRLFSVGEGLGFVTCRKGEGEYTVGV
                     FNNSLEARPFQIRSLAGMIEDIREIPLDQSEKGQPGYWPNGIQHDGGVSDEATIAGND
                     VRIFQVKLAKEAFRALPRVAPPERPRNRFLRLPRETGNVKEAILRMPTFFQHFDGVLL
                     DWNALHGRDVNVLSEEAEWIKRQKVRIAVDFSSGCNLFPDLQLLDTFLPAWARTVAVF
                     DDVFGKMEVLGARDALLELHIGPENEVSHERAHERFLVCVRELCERASARSITLHLRP
                     HPHKFCSTAASMRQFVKEVGATDLRVAVDFGHGAQSEKPEAPDFEGVSFVLVSAPLFD
                     LFGRPFDAHLPVAGSGIDLSALAGAPAEAVIAYDAEYSGWEDIYRDTKVIEEAAGRGG
                     M"
     gene            complement(1556..4000)
                     /locus_tag="PLT86_07810"
     CDS             complement(1556..4000)
                     /locus_tag="PLT86_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36555.1"
                     /translation="MRLSKRQGLVFVFATVVVGCLVRFAAAEDLPGVFTDRIYAWGAS
                     TKDSDAFLKTANSANLAWGESYVLRAFMSAYRLTGDTYWLRRVCTHADSLIAHARDFP
                     EGEYYDPVYADGYLGWGCKNYSDQYDEYMVHDGHVTTPVAAFVAEVYKDSTLWDEFGA
                     RAQTYFAFIADNIAAKWLAITPRTSSGTGETLRLWRGWSFLPHNQYAAFASLLLFLDD
                     AANTPRFAAVHPERVTTVYGQRAREMGTYFRSRLQLNPASNSYSWAYLGYGSGEDASH
                     ANLELELAWWLNERGVVFTDLDMERFARTLTTRMWNKSFNPPLVNKSTEGNDDWTGAE
                     GVWGWSLYSKFDPLLWRILERYYSALPTVRHHQVATLAHLQRFRDVFLNSGIILPEGV
                     IVIDDGDQVLRPGERAKIGISVRNVGVQRDSAKVWLSQYAPLVIAPGGDTTSVVVEPG
                     ASARATFDVQVPGDVATRQITIVGYADFLPATGSVTTGYPDVAFVEGLPGADGLAHNQ
                     FYSSLTTVAHRWVHSETDTFTTVALRNFDAIVWRAAWAVPAPQQRDSLAKFLDMGGNL
                     LITGGGVLPAWIGRSTEDSLFFARYFKVTGAIDVHDSLQGLTPSVRAKNLAALSAIGG
                     SKRNFRTSPPIHFDVLHTFAPEDQTIIFAATDAQLVPKMLPDSVGGVGIDSTYNALLF
                     GWDFDDVSSVIATKSMITYLLAWFKSPTSVESGGTMRPTPTSALRIVALPNPFNPATT
                     ITARGLAQGIAARIAVTNVLGQECWSSTNARPGPEWSVVWNGRNQRGESMGTGTYFVV
                     IEQGDARKATRIQLVR"
     gene            complement(4150..6597)
                     /locus_tag="PLT86_07815"
     CDS             complement(4150..6597)
                     /locus_tag="PLT86_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36556.1"
                     /translation="MGKWRGHICAAIGVMWLSLAVPTSTASDVPGDFMARMIAWDGKY
                     ADSYHRTRDSGTLAWGESYVLRAFMSAYHVTKDPYWLGRVCTHADSLIVYAWDHPDTG
                     YYDPAYADGFLGWGTAHYSDQYDEYMVHEGHVMTPVAELVAEVFKDSTLWEEFGGRAQ
                     TYMAFLTDHIAAKWLRLWPGTPPASFKWGLTVREWYGLDYAPHNQYAVFGAMLLFMDD
                     VVRTARYAQLKPGDPPTIYRQRAVEMGEYFKRHLLYLPAYDAYSWKYHNNSGAEDASH
                     ANLELEFAWWLNERDLVFTDLDMQRFARTLTSIMWNKSLTAPAVNMSTAGTGDWSGTD
                     YLWGWSLFGKFDPLLWRILERYYAAKTGDTSPYGMATVARLQRFRDTFLNHGILVPER
                     LSVIDDGDRVIRPGETARIGVTVRNIGNARDSIIVRISGLDPLVIASGTDSVIAAVDS
                     GAATLVEFVVRAPVDVTTKRVSITARVGSLSVTGQVTVGSPSTVFVEGTPPADDGITH
                     NAFYAALTPPVHRWTWAEGDTFSTAALFRFESVVWRAAWALPTPQQRDTLARFLDMGG
                     NLLITGGGVLPAWITRSPEDSLFFARYFKVTGAVDVHDSLAGLGVSVRPFDPMAFTAM
                     GTSRRYLRSSPVVHFDVLNSFGPPQKQIIFSATDAQFNPKVLPDSIGGVGIDSTYRAL
                     LFGWDLDDMSSNTSRTVVTLALTWLRSPTSVRAGEYPRPDAVREIELSVAPNPFNPHT
                     TITARGLKLGEPARISVVNVLGQVCWSARNPRPGTEWRVMWDGRDVQGEFVGTGTYFV
                     AIEQGDARKATRIQLVR"
     gene            complement(6590..7726)
                     /locus_tag="PLT86_07820"
     CDS             complement(6590..7726)
                     /locus_tag="PLT86_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36557.1"
                     /translation="MTRNALIEALKRLYVACLVDWDRNHADKLVTSDDSAELGWGEAY
                     VLRAFVGAYRFTGDVYWIRRLCAHAGAVLANAKDTPDGKPFDPVYADGFLGWGTYRYS
                     GQYDEYMVHDGHICTPIAEFAAMVFADGRLYEVFGEDATRFVRFIENHIAAKWLNVWD
                     RRRDGDEEFAWGETVRNWGGLDFIPHNQYAAFGALLVFLDDVVRDPRYSSVMGAAAQT
                     VYRQKAIEMGAYFKAHVESLPGTDAYAWKYHDTCGAEDTSHANLELEFAWWLHERGLV
                     FSDSDMDRFAGTFANVMWNGDAEDPRVNASTDGKGDWTGGKTTWGWALFSRRDPALWH
                     VLALTFLSESEAPTGSGLVAMGRLLEACTEAGASLPTRGGMQRG"
     gene            complement(7771..9747)
                     /locus_tag="PLT86_07825"
     CDS             complement(7771..9747)
                     /locus_tag="PLT86_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36558.1"
                     /translation="MRPPWTSSTPFTYEIGYGQDYVGNEQFLSDVRRGRPTLLHCGHD
                     VPLNSATGNTIQFTPRDMKYLDPEGIPARMSELRQYVDSLHEAGVKWAVPYLITMLII
                     GDPEKRTGFWNLYDRWEEYSALGIGEKPGPPESWFSREPRPFPGHPGMLACETTISHP
                     EVRRFLHACTDLVARCGYDGVFLDVNTLIGRTPHDDRWFGEYLSTRYSESELKRRFGF
                     ASLGDVRLGEEGAGLLWVETQRFRAWAFGQLFAALRDAGREHVPGFFVLPNNSPMCTI
                     EGFFSRRGVGHGMRYIRNGCDLLMFEEMQQAGRFGADRIVDNILQYKMAAAHGMRGVV
                     LLYNATDRAAINLANAEAAAGGGGAFVQCGFAEADTMRFWREWFERHSSVLEGMDSLH
                     TVGVLHSLDEMYWDNRAHLEALYRLRQSLSDHHVLFDILLPEHLEQGKTSSFRILVLP
                     EVRHLSNDQVAGMARFCRNGGKVILLGECGTFDETGARRDEKLCDVLDVSATSQVRSI
                     SDIVPTWGPELYELSEEDYNDFDLVARLPDRVASASEAEQVRRSALVPLLDTLLGEPC
                     TFLQDAPYSLRVSAFRSPDGRRTVVHLVNYDLPVLGKGMSGNPIPAGPARIRYGGQRA
                     HWFTPEGVEGDAKRGGDGSFVIPAVETYCMVVIE"
     gene            9881..10414
                     /locus_tag="PLT86_07830"
     CDS             9881..10414
                     /locus_tag="PLT86_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36559.1"
                     /translation="MTDTRTRVDELIEESYLISLPEDAIRKDVLQFLDSCERIAAQTR
                     DESKRQEMQRRLKMFRQLGLRLDSAAELSEEIDQWMAWSPHDMLAHRAARLKLLVLDA
                     LAAVDPSVPSRVGKERFPQSGARGASDTGPVSRSNILPYPRRRKVARKLAVWALRVAY
                     GILLLSAIAVFTYRFLR"
     gene            10427..11089
                     /locus_tag="PLT86_07835"
     CDS             10427..11089
                     /locus_tag="PLT86_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36560.1"
                     /translation="MPRFQVLRAIAGVLIGIAGLVSTPEAAVSSYYVCEQAAYPVNSM
                     GKAYGGSPALEAGLRFGSSSVDYRVSTAYTMLVSASDTLPAVVHHGSVAGDVIFPRAF
                     GAERLYGFAGIGFGTRRLPEGRIVYDERKVDAPWSPYGYYVVQDTVSGGFGARVFDLI
                     GRAGIGYVFHESPRWRGAVEVRYQAAFGVGNPPGITLDMRTDETIGAGVTIEWVRAYR
                     HR"
     gene            11103..>11718
                     /gene="carB"
                     /locus_tag="PLT86_07840"
     CDS             11103..>11718
                     /gene="carB"
                     /locus_tag="PLT86_07840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513329.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOT36561.1"
                     /translation="MPRRTDIHRIMLIGSGPIVIGQACEFDYSGTQACRALRTQGYEV
                     ILVNSNPATIMTDPDFANRTYIEPITADVCAKIIEREKPDALLPTVGGQTALNTAVEL
                     AESGVLEKHGVELIGAKLEAIHKAEDRKLFREAMRRIGVDLPRSGYAYSLAEARKIVE
                     TTGFPAIIRPSYTLGGTGGGIAYNTEEFGDVVSNGLELSPIHEVL"
BASE COUNT         2411 a         3604 c         3387 g         2316 t
ORIGIN      
        1 gcccgtttgt gatgctgcag catgtgcggc gagtgatcgg tgatgcgttg gcgacgcagc
       61 ggttgttttc ggtgggcgag gggctgggat ttgttacgtg ccgaaaagga gagggcgagt
      121 acacggtcgg cgtgttcaac aactccctcg aggcgcgacc gttccagata cgttccttag
      181 cgggcatgat agaggatatc cgtgagattc cgcttgatca atccgaaaaa ggacagccgg
      241 ggtactggcc caacgggatc cagcacgacg gaggcgtatc agacgaggca acgatcgccg
      301 gcaacgacgt gcgcatcttc caggtgaagc tggcgaagga agcttttcgc gcgcttccac
      361 gcgtcgcacc gcccgagcgg ccgcgaaaca ggttcctgcg gttaccacgc gagacgggca
      421 acgtcaagga agcgatcctc aggatgccaa ccttcttcca gcatttcgac ggcgtgctgc
      481 tggattggaa cgctctgcat ggccgggatg tgaacgtcct gagcgaagag gccgaatgga
      541 tcaagcgcca gaaggtgcgc atcgccgtgg atttcagttc cgggtgcaac ctgttccccg
      601 acctgcagtt gctcgatacg tttctgcctg cgtgggcgcg cacggtcgcg gtatttgacg
      661 atgtgttcgg aaaaatggag gttctcggcg cgcgtgatgc tctcctcgag ctgcacatcg
      721 gaccggaaaa cgaagtgagc catgagcggg cgcacgagcg gttcctcgtg tgcgtgcgcg
      781 agctctgcga acgcgcatcg gcccggtcaa tcacgctcca cctgcggccc cacccgcaca
      841 agttctgttc cacggctgcg tctatgcggc agtttgtgaa ggaagtgggg gcaacggacc
      901 tgcgcgttgc cgttgatttc gggcatgggg cgcaaagtga gaagcctgag gcgccggact
      961 tcgagggcgt gtcgttcgtc cttgtctccg cgccgttgtt cgacctgttc ggacggccgt
     1021 ttgacgccca cctgccggtt gcgggaagtg gcatcgatct ctccgcgctt gccggggcgc
     1081 ccgcggaggc cgtcatcgcc tacgatgcgg aatactctgg gtgggaagac atctaccggg
     1141 atacgaaggt gatcgaggag gcggcggggc ggggcgggat gtgacggatt cgtccgatag
     1201 gccctctttc gcccggcccg ttgagccggg ctatggtact gcgccccgtc ggggctcgcc
     1261 tgagtcttgt gacgaccgcc aaggcctgaa gggccgacat aactcggccc gcgggcaccg
     1321 ccccgggcga atcgtcattc acggccaggc aagaacgcct gccccaccga cccgcaaacg
     1381 ggtgggcagg ctgcctgaca aggcagcaag gcattcctgc ctgtccacgg tgcaaccgag
     1441 cctaatcccc actgcgttcg ctgtctcgtt catgtctctt cgagggccgc tcgcgcgctc
     1501 tgcgctccgc tttcgaacac cgcacggcgt tcccccctgc ctcccgtcgt accggctacc
     1561 gaaccagttg gattcgcgtt gcctttcttg catcaccctg ttcgattaca acgaagtacg
     1621 ttcccgtgcc catggactcc ccacgctggt ttcgaccatt ccagacgacg ctccactccg
     1681 ggccaggtcg tgcattcgtc gaactccaac actcctgccc gagcacgttg gtcacggcga
     1741 ttcgcgcagc gatcccctgc gcgaggccgc gagcggttat cgtggtagcc ggattgaacg
     1801 ggttcggcaa tgcaacgatt ctgagtgcac ttgtcggcgt cgggcgcatc gttccacccg
     1861 actccaccga cgtcggcgat ttgaaccatg cgaggaggta agtgatcatc gactttgtgg
     1921 caatcaccga ggacacatcg tcaaaatccc acccgaacag cagtgcgttg taggtggaat
     1981 caatgcccac tccgccgacg gaatcgggaa gcattttcgg aaccaactgg gcgtctgtcg
     2041 ccgcgaagat gatggtctgg tcttcgggcg caaatgtatg cagaacgtcg aaatggatgg
     2101 gtggcgaggt acggaaattt cttttggacc ctccgattgc gctgagcgcc gccaggtttt
     2161 tcgcacgaac gctcggggta agcccctgga ggctgtcgtg cacatcaatc gcgccggtca
     2221 ccttgaaata ccgggcgaag aacagcgaat cctccgttga ccgcccaatc catgcgggca
     2281 gaacaccgcc acctgttatg aggagattgc cacccatgtc gaggaatttt gccagcgaat
     2341 cccgctgctg aggagcaggc accgcccacg cggcgcgcca tacgatcgcg tcgaagttcc
     2401 gcagggcaac cgtcgtgaag gtgtcggttt ccgaatggac ccagcggtgg gcgacagtag
     2461 tgaggctgga atagaactgg ttgtgggcga gtccatcggc gccagggagc ccttccacaa
     2521 acgcgacgtc cgggtacccg gtggtgacgc tccccgtagc gggtaagaaa tccgcgtaac
     2581 ccacgatggt tatctgtctg gtcgcaacgt ccccgggaac ctgaacatca aatgtggctc
     2641 gggcagaggc ccccggctcg accacgacag aagtggtatc accccccgga gcaatgacga
     2701 ggggagcgta ttgcgataac cagaccttcg ccgaatcacg ctgcacgcct acattccgca
     2761 ctgagatacc gatcttcgcc ctttctcccg gtcggagtac ctggtcacca tcgtcgatca
     2821 caataacgcc ttctggcaaa atgatcccac tgttgaggaa cacgtcgcgg aaacgctgca
     2881 ggtgagccag tgttgccacc tgatggtgtc gcacggtagg gagcgcgctg tagtaccgtt
     2941 ccaggattcg ccagagcaac ggatcgaact tcgaatagag ggaccacccc cagacgcctt
     3001 ccgccccggt ccagtcgtcg ttgccttcgg tgctcttgtt caccagcgga ggattgaagc
     3061 ttttgttcca catcctcgtc gtcagcgttc gggcaaagcg ttccatgtcg agatcagtga
     3121 acacgacgcc gcgttcattc agccaccatg ccagttccag ctccagattc gcatgggacg
     3181 cgtcctcgcc cgaaccgtat ccgagatagg cccaggaata cgagttcgat gccgggttga
     3241 gctgcaggcg actccggaaa taggtcccca tttcgcgcgc ccgttgcccg tacaccgtcg
     3301 taactcgttc cgggtgcacc gcggcaaatc gcggcgtgtt agccgcgtcg tcgaggaaga
     3361 gaagcaaaga agcaaacgcc gcatactggt tgtggggcag gaacgaccag ccccgccaaa
     3421 gccgcagtgt ttcccctgtt cccgaggaag tcctcggcgt aatcgccagc catttggcgg
     3481 caatattgtc cgcaatgaac gcgaagtacg tctgcgcacg tgccccgaat tcgtcccaca
     3541 gagtggaatc cttgtatact tcggcgacaa acgcggcaac gggagtcgtc acgtgcccat
     3601 cgtgcaccat gtactcgtca tactgatcgg agtagttttt gcacccccac ccgagatacc
     3661 cgtccgcata caccgggtca taatactccc cttccggaaa atcccgcgca tgggcaatca
     3721 gcgagtccgc gtgtgtgcac acgcggcgaa gccagtaggt atctcccgtc aaacggtagg
     3781 cgctcatgaa cgcacgaagc acgtacgatt cgccccacgc gagattggcg gagtttgccg
     3841 ttttcaggaa cgcatccgaa tccttcgtgc tcgcgcccca ggcataaatc cggtccgtga
     3901 acactccagg aagatcctct gccgccgcaa accgaacgag acaccccaca acgaccgtcg
     3961 cgaaaacgaa caccaacccc tgcctttttg acaacctcat tccctcaccc ctcgcttttg
     4021 atcggccctc tcttgcggaa cggttccccg caacgaactg cttgtttgaa agcttccccc
     4081 ggaaatcacg cgtgcgttcg cgggaattca agggatgcac cacacacctc tggtccccaa
     4141 atctgccaac taccgaacca gttggattcg cgttgccttt cttgcatcac cctgttcgat
     4201 ggcaacgaag tacgttcccg tgcccacgaa ctccccctgc acgtcccgtc catcccacat
     4261 gacgcgccat tccgtaccgg gccggggatt tcgcgcgctc caacacacct gtcccagaac
     4321 gtttaccaca ctgatccgtg caggttcccc cagcttgagg ccgcgagcgg ttatggtcgt
     4381 atgcgggttg aacggattcg gtgccacgct gagttcaatt tcgcgaaccg cgtcgggtcg
     4441 tgggtattct ccggcccgga ccgaggttgg cgatctcagc cacgtgaggg cgagcgtcac
     4501 aacggttctg gacgtgttgg aggacatatc atccagatcc caaccgaaca acagagcccg
     4561 gtacgtactg tctatcccta cgccgccaat cgaatcgggc aggactttcg ggttgaactg
     4621 ggcatctgtc gcggagaaaa tgatctgctt ctgcggcgga ccaaaggagt taagaacgtc
     4681 gaaatgaacg acgggtgaac tccggaggta tctcctggac gtgcccattg cagtgaaggc
     4741 catgggatca aaggggcgaa cagaaacacc cagtccggcg aggctgtcgt gcacatcgac
     4801 tgcgccggtt accttgaaat agcgggcgaa aaagagggaa tcttccggcg atcgcgtaat
     4861 ccaggcgggc aagaccccgc cgcccgtgat caggagattg ccccccatat ccaggaaccg
     4921 tgcgagcgtg tcacgttgct gtggggtcgg gagcgcccac gcagcgcgcc agaccacaga
     4981 ttcaaatcgg aaaagcgccg ccgtcgagaa cgtgtcccct tccgcccatg tccagcggtg
     5041 caccgggggt gtcagcgcag catagaatgc attgtgcgta attccgtcgt ccgcgggtgg
     5101 tgtcccttcc acgaaaacgg tagatggtga accaacggtc acctgaccgg tgactgacag
     5161 tgaaccaacg cgggccgtaa tggacacccg tttcgtcgtg acgtcaacag gcgcacgaac
     5221 gacaaactcc accagcgtag cagcgcccga atccaccgct gcgatgaccg aatcggtccc
     5281 ggatgcgatg accagaggat caagacccga aatccgaaca atgattgagt cccgcgcgtt
     5341 cccgatattg cgaacggtga caccgattct ggccgtctct cccggacgaa tcacacgatc
     5401 gccatcgtca atgacactca atcgttcggg aacaagaatg ccgtggttca gaaatgtgtc
     5461 acgaaaccgc tgaagccttg caacggtcgc cataccgtag ggcgaggtat ctcctgtttt
     5521 tgcggcgtag taccgttcga gaatcctcca caggagagga tcgaacttgc cgaagaggga
     5581 ccaaccccag agataatccg tccccgacca gtcacctgtt cccgcggtgc tcatgttcac
     5641 ggcgggtgca gtgaggcttt tgttccacat gatggacgtc aaggtgcgcg cgaagcgctg
     5701 catgtcaagg tcggtgaaga ccaggtcacg ctcgttcagc caccacgcga attccagttc
     5761 gaggttcgcg tgcgacgcgt cttcggctcc gctgttgttg tggtatttcc acgagtaggc
     5821 gtcgtacgcg ggcaggtaga gaagatgccg tttgaagtat tcgcccatct ccactgcgcg
     5881 ttggcggtag atcgtgggcg ggtcacccgg tttcaactgc gcataccgtg cggttcgaac
     5941 aacgtcgtcc atgaacagca gcatcgcgcc gaacaccgcg tattgattgt ggggtgcgta
     6001 atcgagcccg taccactcgc gcactgtgag accccattta aagctcgctg gcggcgtgcc
     6061 tggccacagg cgcagccact tcgctgcaat gtggtcggtg agaaaggcca tatacgtctg
     6121 cgctcgcccg ccgaattcct cccacagtgt cgagtccttg aacacctccg cgaccagctc
     6181 agcgacgggt gtcatcacgt gcccttcgtg caccatgtat tcatcgtact ggtcagaata
     6241 atgagccgtt ccccagccaa gaaatccatc ggcgtaggcc gggtcgtagt atcccgtgtc
     6301 cggatgatcc catgcgtaga ctatcaacga atccgcgtgg gtacacactc gccccaacca
     6361 gtacgggtct ttcgtcacgt gatacgcact catgaatgcg cgaagaacgt acgattcccc
     6421 ccacgccaga gtcccggaat cccgagttct gtggtatgaa tcggcgtatt tcccgtccca
     6481 cgcgatcatg cgggccatga aatcgccggg cacgtccgat gctgtcgagg tcggcacagc
     6541 cagactcaac cacatcacac cgatcgctgc acagatatgt ccgcgccatt tacccacgct
     6601 gcattcctcc tctggttggc aggctggctc ctgcctcggt acaggcttcc aacaaacggc
     6661 ccattgccac aagcccgcta ccggtcggcg cttcagactc cgaaagaaac gttagggcga
     6721 gaacgtgcca cagagcgggg tcgcgccggg aaaagagtgc ccatccccat gtcgttttgc
     6781 ctcccgtcca atcacctttt ccatccgtcg acgcattcac gcggggatct tccgcatcgc
     6841 cgttccacat cacgttggca aacgtcccgg cgaaccggtc catgtcgctg tcggagaaca
     6901 caagtccgcg ctcatgcaac caccaggcaa actccagctc gaggttggcg tgggaagtgt
     6961 cttcggcgcc acaggtgtcg tggtatttcc acgcgtacgc atcggtcccc ggaagcgatt
     7021 cgacatgggc tttgaagtac gcgcccatct caatggcctt ctggcgatat acggtctgag
     7081 cggcggcacc catgaccgaa gagtaacggg gatccctgac cacgtcatcc agaaacacga
     7141 gtagagcgcc aaaggctgcg tactggttgt ggggaatgaa atccagtccg ccccagttcc
     7201 gcactgtctc tccccatgcg aactcctcgt caccgtccct tcgacgatcc cacacgttga
     7261 gccattttgc agcgatatgg ttctcaatga acctgacaaa ccgcgtcgca tcttccccga
     7321 acacctcgta cagacgccca tcagcgaaca ccatcgcggc aaattccgca attggagtac
     7381 agatgtgccc gtcgtgcacc atgtattcgt cgtactggcc agagtacctg tacgttcccc
     7441 acccaaggaa cccgtcagca tacaccgggt cgaacggttt gccgtccggc gtgtccttcg
     7501 cgtttgccag tacggctcct gcgtgcgcac acagccttcg aatccagtag acgtcgccgg
     7561 taaagcgata ggctccaacg aaagcccgca ggacgtacgc ttcgccccat cccagttccg
     7621 cagaatcgtc actggtgacc agtttgtcgg cgtggttcct gtcccaatca accagacaag
     7681 ccacgtagag tctcttgaga gcttcgataa gcgcattcct ggtcatttgt cccccgaatt
     7741 atcgcgctac gggcatgttc ggccatccac tcactcaatc accaccatgc agtaggtctc
     7801 aacagcgggt atgacaaaac tcccgtctcc gccgcgtttc gcatcgcctt ccacaccctc
     7861 gggagtgaac cagtgtgcgc gttgacctcc gtaacgtatt cgcgcgggac cggcaggaat
     7921 cgggttaccg gacatccctt tcccgagcac gggcaggtcg taattcacca gatgaactac
     7981 ggttcgcctc ccgtctggcg aacggaacgc cgaaacgcgg agactgtaag gcgcgtcctg
     8041 cagaaacgtg cagggttcac ccagaagtgt gtcgagaagt ggtaccagcg ctgaacgccg
     8101 cacctgttcg gcctccgaag cggaagcaac gcggtcgggc aggcgggcga cgaggtcgaa
     8161 gtcattgtag tcctcctcgc tgagttcgta cagctccggt ccccacgtcg gaacgatatc
     8221 tgatatcgaa cgcacctgcg aggttgcgga cacatcgagc acatcacaca gcttttcgtc
     8281 tcgtcgtgct cccgtttcat cgaacgttcc gcattcaccg agcaggatta ccttcccgcc
     8341 attccggcaa aacctcgcca tcccggccac ctggtcattg ctcagatgcc gcacctcggg
     8401 aagaacgaga atccggaacg acgaagtctt cccctgctcc agatgctccg gcagcaggat
     8461 gtcgaacagc acgtggtgat ccgacagaga ttgccgaaga cggtacagcg cctcgaggtg
     8521 ggcgcggtta tcccagtaca tctcgtccaa cgagtggagt acgccgaccg tgtgaagcga
     8581 atccattcct tccaacacac tggaatgccg ctcgaaccac tcgcgccaga acctcatggt
     8641 atcggcttcg gcaaacccgc actgaacaaa tgcgccacct ccaccggccg ccgcttcggc
     8701 attggcgagg ttgattgccg ccctgtccgt cgcattgtac aggagtacaa cacctctcat
     8761 cccgtgcgcg gctgccattt tgtattgcag gatattgtca acaatccggt cggcgccgaa
     8821 tctgcccgcc tgctgcatct cctcgaacat cagcagatcg catccgttcc ggatgtaccg
     8881 cataccgtgg ccaacgcccc tgcgcgagaa aaatccctcg atggtgcaca tcggcgaatt
     8941 gttcggaagc acgaagaacc caggcacgtg ctcgcgtccc gcatctcgga gcgctgcgaa
     9001 aagctggccg aacgcccacg ctcggaaccg ctgtgtttcc acccacaaca ggccggcacc
     9061 ctcttctccc agtctcacgt cgccaagtga tgcgaatccg aatcttcgtt tcaactcgct
     9121 ttcggagtac cgggtcgaca ggtattcacc aaaccagcga tcgtcgtgag gggttctccc
     9181 tataagggta ttgacgtcga gaaaaacacc gtcgtacccg catcgagcca caaggtcggt
     9241 gcacgcgtgc aggaaacgcc gcacctccgg gtgcgagata gttgtttcac acgccaacat
     9301 gcccgggtgg cccgggaaag gacgcggctc acgcgagaac caactctctg gtggtccggg
     9361 tttttccccg atgccgagag ccgagtattc ctcccaccgg tcataaaggt tccagaatcc
     9421 ggttcttttc tccgggtcac cgatgatgag catcgttatg aggtacggaa ccgcccactt
     9481 cacgcccgcc tcgtgtaggg aatccacgta ctgacgcaac tcggacatgc gggcaggaat
     9541 accctcaggg tccaggtact tcatgtcccg gggagtgaac tgaattgtgt tgcccgtagc
     9601 gctgttaagc ggaacgtcat gcccgcagtg cagcagagtc gggcgcccgc gcctcacatc
     9661 tgacagaaac tgctcgttgc cgacgtagtc ctgcccgtaa ccgatctcgt acgtgaacgg
     9721 ggtactgctt gtccatggtg gtctcatcgg cgtctccttt ccccttcgac taaggtagcg
     9781 cggcgacgat gctcatacaa agggcgtcag cttgaccgtt ttgccgcccg tggcgtacat
     9841 tcttttgtgc ccggtgtacc ggattgaaga ggcagcacca atgacggata cgcgcaccag
     9901 agtcgatgag ttgattgagg aatcgtacct gatttctctg cccgaggatg ccattcggaa
     9961 ggatgtcctt cagtttcttg actcgtgcga aaggatagcg gcacaaacgc gtgacgaatc
    10021 gaagcggcag gagatgcagc ggcgactgaa gatgttcaga caactgggcc ttcgtctcga
    10081 ttctgccgcg gaactgagtg aggagataga ccagtggatg gcctggtcgc ctcacgacat
    10141 gcttgcgcac cgggccgcaa ggttgaaact gctcgtgctc gatgcacttg cagcggtcga
    10201 cccgagtgtc ccatcacgcg tcgggaagga aagattccct cagagtggcg cacgtggcgc
    10261 gagcgacacg ggtcccgttt cccgttcgaa cattctcccg tatccaaggc gacgcaaggt
    10321 cgcgcgcaaa ctggcggtgt gggcattgcg ggtggcctac gggatccttc tcctctcggc
    10381 gatagccgtc ttcacgtaca gattccttcg ttaaggagtg ggtgcgatgc cgcgattcca
    10441 ggtactccgg gcgatcgccg gagtgctgat tggcattgca ggcctcgtga gcactcctga
    10501 agccgcagtg tcttcatact acgtgtgtga gcaggctgcg tatccggtga actccatggg
    10561 taaggcgtat ggcgggtccc ccgcattgga ggcgggtttg cgtttcggct cctcgtcagt
    10621 ggactaccgc gtgtcaacgg catacaccat gctggtgtct gccagcgata cgttgccggc
    10681 cgtcgtacat cacgggagtg tcgccggcga cgtgattttt ccccgcgcgt ttggcgccga
    10741 gcgattgtac gggttcgccg ggatcggctt cggcacccgc cgtctcccgg aagggcgcat
    10801 cgtgtacgac gagcgtaagg tggacgctcc ctggtcgcct tacggctatt acgtagtgca
    10861 ggataccgtt tcgggtggat tcggcgcacg cgtgttcgac cttatcgggc gtgccggaat
    10921 agggtatgtg ttccacgaat ctccgcgttg gcggggggca gtcgaggttc ggtaccaggc
    10981 ggcgttcggc gtcgggaatc cgcccggaat caccctcgac atgcgcacgg acgagacgat
    11041 aggtgcggga gtgacgatcg aatgggtcag agcgtaccgg caccgatgac aaacgaggaa
    11101 tcatgccgcg aagaacagac attcaccgaa taatgcttat cgggtccggc ccgatagtaa
    11161 tcggtcaggc gtgcgagttt gactactcgg gtactcaggc ctgcagggca ctgagaactc
    11221 aggggtatga ggtgatcctc gtcaacagca accctgcaac catcatgacg gacccggatt
    11281 tcgcaaaccg cacctatata gagcctatca ctgctgacgt gtgcgcgaaa atcatcgagc
    11341 gcgagaaacc ggatgcattg ctgcccaccg tcggcggcca gacggctctc aacacggcag
    11401 tggaactggc cgaaagcggc gtactggaaa agcatggtgt cgagttgata ggtgcgaagc
    11461 tggaagccat tcacaaagcg gaagaccgca agctgtttcg cgaggcgatg cgcagaatcg
    11521 gtgtcgacct gccgcggagt ggatacgcgt actccctcgc agaagcccgg aagatagtcg
    11581 aaactacggg tttcccggca ataatccgac catcctatac actcggagga actggaggag
    11641 ggatcgcgta caacacggag gaattcggcg acgttgtctc aaatggtctc gagctcagtc
    11701 ccattcacga ggtcctta
//
LOCUS       DAOSOO010000090        11606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5532_length_11606_cov_6.820968, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000090 DAOSOO010000000
VERSION     DAOSOO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5532_length_11606_cov_6.820968"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="fusA"
                     /locus_tag="PLT86_07845"
     CDS             <1..1501
                     /gene="fusA"
                     /locus_tag="PLT86_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT36562.1"
                     /translation="RDMVELANQYREKLLEAVADFDDVVMAKFLDGNHDDITEAEIRA
                     AVRKGTISLQIFPVLCGSAFKFKGVRRLLDAVVDYLPSPLDVPPVKGSALDDEEVTIT
                     RRASEDEPFSALAFKVMTDPYVGRLTYLRVYSGALTTGSYVYNSTTRRRERIGRLLQM
                     HSNKREEIDVVYAGDIAAAIGLKNTTTGDTLCDEQHPVVLERMVFPEPVIDVAIEPKT
                     KADQEQLGASLNKLAEEDPTFRLRQDTETGQTIISGMGELHLEIIVDRLRREFRVEAN
                     VGRPQVSYKEAFTRMVEAEGRYVRQTGGRGQYGHVWVRFEPGEPGTGFVFVDATVGGS
                     VPKEYIKPVEEGIRQAMESGPLAGYPVVDLKATLFDGSYHEVDSSEVAYKIAGSLAFR
                     EAGRKAEPVLLEPIMKVEVIVPGDYLGDVIGDLNSRRAQIHSITPRPDAQVVEANVPL
                     SEMFGYATALRSATQGRAIYTMEFSKFQPAPKSITDEIVTKMTGAKSAA"
     gene            1521..2711
                     /gene="tuf"
                     /locus_tag="PLT86_07850"
     CDS             1521..2711
                     /gene="tuf"
                     /locus_tag="PLT86_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT36563.1"
                     /translation="MGKAKFERTKPHVNVGTIGHVDHGKTTLTAAITQVLSLRGLAQA
                     KGYDEIDNAPEEKARGVTINVHHAEYETATRHYAHVDCPGHADFIKNMITGAAQMDGA
                     VLVVSAADGPMPQTREHILLARQVQVPYIVVFLNKTDMVDDPELLDLVELEVRELLTK
                     YEFPGDEIPVIRGSALIALENPTDPEKTQCITELMDALDSYIPLPERAVDLPFLMPVE
                     DVFSITGRGTVGTGRVERGRISTGNEVEIVGIRETRKTVVTGVEMFRKILDDAQAGDN
                     IGVLLRGIDKKDLERGMVLAKPGSITPHVKFEAEVYVLTKEEGGRHTPFFGGYRPQFF
                     FRTTDVTGSIALPEGTEMVMPGDHTVMTVELVTPIAMEQGLRFAIREGGRTVGAGSVT
                     KILQ"
     gene            2762..2914
                     /gene="rpmG"
                     /locus_tag="PLT86_07855"
     CDS             2762..2914
                     /gene="rpmG"
                     /locus_tag="PLT86_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT36564.1"
                     /translation="MPRDIITLACEDCKRRNYSGDKNKRKHSERVTYKKYCPWCKKHT
                     PHRETR"
     gene            2939..3016
                     /locus_tag="PLT86_07860"
     tRNA            2939..3016
                     /locus_tag="PLT86_07860"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2974..2976,aa:Trp,seq:cca)
     gene            3046..3231
                     /gene="secE"
                     /locus_tag="PLT86_07865"
     CDS             3046..3231
                     /gene="secE"
                     /locus_tag="PLT86_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT36565.1"
                     /translation="MFQKVATYYREVVMEMGKVSWPTRDQLKNSTIVVLIVTAIFAVF
                     IGAFDWILSQIVQWFLR"
     gene            3235..3819
                     /gene="nusG"
                     /locus_tag="PLT86_07870"
     CDS             3235..3819
                     /gene="nusG"
                     /locus_tag="PLT86_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT36566.1"
                     /translation="MTDTPQGEAPRPKRWYAVHTLSGHENKVLHYLKNSAEALALGDQ
                     IGEVVIPIEDVPEMREGKRVTTKRRFLPGYVLIEMELTSQSRYVVENTPGVTGFVTTG
                     GRSSKEPAPLRPSDVERILQQVERKSTAPETDSAYRPGDFVKVVDGPFSDFVGVVEAF
                     DTAKGKVTIAVSIFGRPTKVELDLLQIEPAPPKA"
     gene            3915..4352
                     /gene="rplK"
                     /locus_tag="PLT86_07875"
     CDS             3915..4352
                     /gene="rplK"
                     /locus_tag="PLT86_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT36567.1"
                     /translation="MAKKVIAQIKLQLPAGQATPAPPVGPALSQHRLNIMEFVKAFNE
                     RTRSQQGMIIPAVITAYADSSFTFILKTPPAVTLLKKAAGLEKGSPEPNAKKVGKVTW
                     DQVVEIAKTKLPDLNTTNLDSAASMIAGTARSMGLEVVGRPTK"
     gene            4364..5068
                     /gene="rplA"
                     /locus_tag="PLT86_07880"
     CDS             4364..5068
                     /gene="rplA"
                     /locus_tag="PLT86_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT36568.1"
                     /translation="MPKLAKKYQHDLEKVDRTKSYGLREAIEVIKQFEPRGFDQTVEG
                     VMKLNVDPRKADQLVRGSVVLPSGTGKTVRVLVFARGEAATAAEQAGADFVGADDLVA
                     KIQEGWTDFDVAIATPDLMGVVGRLGRVLGPRGLMPNPKTGTVTQDTAAAVQAAKGGK
                     IDFRVDKAGNVQAPLGRLSFSVDALYANAQVFFEAVMRARPTAVKGQYVHSANLCASM
                     TPSVPLDRNQFQSLVK"
     gene            5091..5681
                     /gene="rplJ"
                     /locus_tag="PLT86_07885"
     CDS             5091..5681
                     /gene="rplJ"
                     /locus_tag="PLT86_07885"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT36569.1"
                     /translation="MPTPKKNETIARLTDMFNSANGIFLTDSTGLKVELVTNLRKRCA
                     AENIGFMVVKNTLARRAAKAAGLPDLDKWFTGPTAVAYSKNNPAGPVKILRAFVREVR
                     EANGKLEIKKGVVDGQVIDDAQMQMLADLPTPEVVRARFLGLLQAPASQFVGILSAGP
                     ASLVRALDQRRQQLDEQAAGSTAGSTEAPTDAPAGQ"
     gene            5697..6080
                     /gene="rplL"
                     /locus_tag="PLT86_07890"
     CDS             5697..6080
                     /gene="rplL"
                     /locus_tag="PLT86_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT36570.1"
                     /translation="MADLQALKTQLSALTVLEIAQLTKELEAEWGVSAAAPVAVAAAP
                     AAGGAAAAAPVEEQTEFTPTLVDVGSQKIQVIKVVRELTGLGLKEAKALVDAGTAPVK
                     EGVSKEEANTIKEKLEAVGAKVEIK"
     gene            6250..10044
                     /gene="rpoB"
                     /locus_tag="PLT86_07895"
     CDS             6250..10044
                     /gene="rpoB"
                     /locus_tag="PLT86_07895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT36571.1"
                     /translation="MPERMIKRRSYAKLPTILDIPNLLELQVASFEDFLQANTPPEKR
                     KIKGLQEVFESVFPITDVHNLYSLEFVSYSIGRPKYDIDECRDRGATYAAPLRAVLRL
                     VTREKGDEKRVKDVIEQVVYLGDLPLMTSTGTFLINGAERVIVSQLHRSPGVFFDEVA
                     HPNGKRLFTARIIPYRGCWVEFTMDINDVMYVHIDRKRKQPVTLLLRALGYSSDLDVL
                     NLFYERITTKLSSKVKGRLLAADVVNAATGEVLADAFATLTDETIAMLEEHGVEEVEV
                     YSPNTEDEIGIIENTLRRDPCQDERGEEDALYRIYQLMRPGDPPNVETARTFVERLFF
                     SPKRYDLGDVGRYRMNQKLDLKIPQDTHVLTREDFISIIRYIIALRNGSPSAQTDDID
                     HLGNRRCRTVGELLANQFSIGLSRMARTIRERMSLRDNENLTPQELVNAKTVSTVIAS
                     FFGSSQLSQFMAQTNPLDELTHKRRLSALGPGGLTRERAGFEVRDVHHTHYGRVCPIE
                     TPEGQNIGLIMSLAAHARINQYGFIETPYRVVKDGVVTTEIRYLSSDEEDRYVIAQAN
                     APLKEDGSFANNLVKARRRGDFPTVSPLEVDYMDVSPIQLVSAAAALIPFLEHDDANR
                     ALMGSNMQRQAVPLLKAEAPLVGTGIERRIALDSGAVTLAKADGEVVYVDSSKIVVRE
                     KKPVHANPLALDEGHERKYSLMKFQRSNQDCCQNQRPLVRPGDKVKAGEVLADGPATD
                     RGDLALGCNVLCAFMPWHGYNFEDAIVVSERLIRNDVFTSVHIEEYELQVRDTKRGQE
                     ELTRDIPNVSDQALRDLDENGIVRVGARVKPGDILVGKVTPKGETELSPEERLLRAIF
                     GEKAGDVRDASLKAPAGMEGVVIDTKIFSRKERDEATRSRNKETIEQIRANMDERAAR
                     VRSALKQRSIAVCQGLTAGNWRSEADGTIVVHEGTVLTEEILSELDLTTLRPDTTWTT
                     DSAANGKIEEMVRYAHDMLAAIQEEFDRESEKVVRGDELPPGVIQLVKVYVAHKRKLS
                     VGDKMAGRHGNKGVVAKVVPVEDMPYLPDGTPVDLVLNPLGVPSRMNLGQILETHLGW
                     AAEKLGIHVSCPVFDGATVAEIKNALKEAGLPESGQTVLYDGMTGERFEREVTVGYIY
                     MMKLIHLVADKIHARSIGPYSLVTQQPLGGRAQFGGQRFGEMEVWALEAYGASHLLQE
                     MLTVKSDDVPGRSRIYEAIVKGENPPEPGIPESFNVLVKELQALCLDVVLQQDGQVAD
                     QS"
     gene            10135..>11606
                     /locus_tag="PLT86_07900"
     CDS             10135..>11606
                     /locus_tag="PLT86_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT36572.1"
                     /translation="MPQQRKPLDFDSIKIQLASPETIRSWSYGEVLKPETINYRTYKP
                     ERDGLFCERIFGPTRDWECHCGKYKRIRYKGVICDRCGVEVTQAKVRRERMGHIELAV
                     SVSHIWYFKSVPSRMSYLLAMTVRNLERVLYYESYVVLDPGNAPLEKGDLISEEQVID
                     LEERGFQFSAEMGAGAIKKMLADLNLDELSTELRETIRKETSAQRKAEALRRLSIVEA
                     FRQSNNRPEWMILDVIPVLPPELRPLVPLEGGRFATSDLNDLYRRVINRNNRLKKLIE
                     IKAPEVILRNEKRMLQEAVDALFDNGRRSRAVRGDANRALKSLGDLLKGKQGRFRQNL
                     LGKRVDYSGRSVIVVGPELKLYECGLPKIMALELFKPFIIHELERRGLVQTVKSAKKK
                     IEQGSTEVWDILEEVIKGHPVLLNRAPTLHRLGIQAFQPVLVEGKAIRIHPLVCKAFN
                     ADFDGDQMAVHVPLSFEAQIEASVLMLSANNLLLPSSGNPV"
BASE COUNT         2478 a         3014 c         3796 g         2318 t
ORIGIN      
        1 gcgggacatg gtggagctgg cgaatcagta ccgcgagaag ctcctcgagg cagtcgctga
       61 cttcgacgac gtcgtcatgg cgaagttcct cgatggaaac catgacgaca tcacagaggc
      121 tgagattcgc gccgcagtgc ggaaagggac gatctccctg cagatcttcc ccgtactctg
      181 cggttccgcg ttcaagttca agggtgtccg gcgtcttctt gacgcagttg tagattacct
      241 cccctctcct ctcgatgttc ctccggtaaa aggctccgct ctcgacgatg aagaagtgac
      301 gataacgcgt cgagcttccg aggacgagcc tttcagcgca ctcgcgttta aagtgatgac
      361 cgacccctac gtaggtcggc tgacgtacct tcgcgtgtac agcggggcgc tgacgaccgg
      421 ttcttacgtg tataactcga ccacccgcag gcgagaacgt atcgggcgtc ttctccagat
      481 gcattcgaac aagcgtgagg agatcgacgt cgtgtatgcg ggtgatattg ctgccgcgat
      541 cggtctgaag aacaccacga ccggcgacac gctgtgcgat gaacaacatc cggtggtgct
      601 cgagcgcatg gtgttccccg agccggtaat tgatgtcgcg attgagccga agacgaaagc
      661 ggatcaggag caactcggcg cctcgctgaa caagctggcg gaagaggatc cgaccttccg
      721 gctccgtcag gataccgaaa ccggtcagac gatcatcagc ggtatgggtg agctgcacct
      781 cgagatcatt gttgaccgtc tccgccggga gttccgggtc gaggccaacg taggccgtcc
      841 gcaggtgtcc tacaaagagg cgttcacgcg catggtcgaa gcggaaggcc ggtatgtgcg
      901 gcagacaggt ggccgggggc aatacggtca cgtgtgggtg cgctttgaac cgggcgaacc
      961 cggaaccggg tttgtcttcg tcgatgccac ggtgggcggt tcggtgccga aggagtacat
     1021 caagcctgtg gaagagggta tccgtcaggc gatggaaagt ggtccccttg ccggatatcc
     1081 ggtggtagac cttaaggcga cgctgtttga cggttcgtac catgaagtgg attcgagcga
     1141 ggttgcgtac aagatcgcgg gttcactggc gttccgtgag gcggggcgca aagctgagcc
     1201 ggtgcttctt gagcctatca tgaaggtcga agtgatcgtg ccgggtgatt atctcggcga
     1261 cgtgatcggt gacctgaact cacggcgtgc gcagatacac agcatcacgc cgcgtcccga
     1321 tgcacaggtt gttgaagcga acgtgccgtt gagcgagatg ttcgggtatg cgacggcgct
     1381 tcgatcagcg acgcagggtc gcgcgattta cacgatggag ttcagcaagt tccagcctgc
     1441 tccgaagtcg attacggatg aaattgtcac caaaatgacc ggcgcgaagt ccgcggcata
     1501 atacagaagg tggtgcaccg atgggtaagg cgaaatttga gcgaacgaag cctcacgtga
     1561 acgtggggac gatcggtcac gtggatcatg gtaagacgac gttgacggcg gcgatcactc
     1621 aggtgttgtc gttgcggggc cttgcgcagg cgaaggggta tgatgagatc gacaacgcgc
     1681 cggaggagaa ggcgcgtggt gtgacgatca acgtgcatca tgcggagtat gagacggcga
     1741 ctcgtcatta tgctcatgtg gactgccctg gccatgcgga ctttatcaag aacatgatta
     1801 cgggtgctgc gcagatggat ggcgcggtgc ttgtggtgtc tgcggcggat ggtccgatgc
     1861 ctcagacgcg tgagcacata cttcttgccc gtcaggttca ggttccgtat atcgtggtgt
     1921 ttttgaacaa gacggacatg gtggatgatc ctgagttgct ggacctggtg gagcttgagg
     1981 ttcgagagct gctgacgaag tatgagtttc ctggtgatga gattccggtg atacgaggtt
     2041 cggcgctgat cgcgctggag aacccgacgg atccggagaa gacgcagtgc atcacggagt
     2101 tgatggatgc gcttgacagt tatatcccgc ttcccgagcg ggcggtggat ctgccgttct
     2161 tgatgccggt ggaagatgtg ttttcgatta cgggtcgggg gacggtaggc accggtcgtg
     2221 tggagcgagg tcggatctcg acggggaacg aagttgagat cgtgggtatt cgtgagacgc
     2281 gtaagacggt agtaacgggc gtggagatgt tccggaagat tctggacgat gcgcaggcgg
     2341 gagacaacat aggcgtgttg cttcgcggta tcgacaagaa ggatctggaa cgtggtatgg
     2401 tgttggcgaa gcctggttcg ataactccgc acgtgaagtt tgaggcggag gtgtatgtgt
     2461 tgacgaagga ggagggaggt cgtcacacgc cgttttttgg cgggtatcgt cctcagtttt
     2521 tctttcggac gaccgatgtg acggggtcga ttgcgttgcc ggaaggcacg gagatggtga
     2581 tgcctggtga ccacacggtg atgacggtgg agcttgtgac gccgattgcg atggaacagg
     2641 gtcttcgttt tgcgattcgt gaaggcggca gaaccgtcgg cgcaggctcc gttacaaaaa
     2701 ttctgcagta agcagggacg cagcgaacga tacttctttt gattgaaagg tgcgcgggcc
     2761 tatgccacgc gatatcatca ctctggcatg tgaagactgc aaacgccgca actattcggg
     2821 cgacaagaac aagcggaagc attctgaacg tgtgacctac aagaaatact gcccatggtg
     2881 caagaagcac acgccgcacc gtgagacacg ataatcactc acactgcgag atacgggtag
     2941 gagtgtagct cagtctggct agagcatcgg actccaaatc cgacggtcgg gggttcgaat
     3001 ccctccactc ctgccattct ccgttaactg gccgggacag gcgacgtgtt tcagaaagtg
     3061 gcgacatatt accgggaagt agtcatggag atgggcaagg tatcgtggcc cacgcgtgac
     3121 caattgaaga actcgactat cgtcgtgctg atagtcacgg ccatctttgc cgtattcatc
     3181 ggtgcctttg actggatttt gagccagatc gttcaatggt ttttgcggta ggttatgact
     3241 gatacaccac agggtgaagc tccgcgcccg aagcgctggt acgcggtgca tacgctttcg
     3301 gggcatgaga acaaggtgct tcactatctg aagaactcgg cggaggctct cgcgctgggc
     3361 gaccagatag gggaagtcgt gatcccgatt gaggatgtgc cggagatgcg cgagggcaaa
     3421 cgcgttacga cgaagcgtcg tttcctgccc gggtatgtcc tcatcgagat ggagcttacc
     3481 agccaatcgc ggtacgtggt ggagaacacc ccaggggtta cgggttttgt tacgacgggt
     3541 ggtcgttcga gcaaggaacc cgcgcctctc cgtccgagcg atgtggagcg cattcttcag
     3601 caggttgagc ggaaaagcac ggcgccggag acggactctg cgtaccgtcc cggcgatttt
     3661 gtgaaagtcg tggatggccc gttcagcgat ttcgttggcg tggtggaagc gttcgacacg
     3721 gcgaagggca aagtgacgat tgcggtgagc atcttcggcc gcccgacgaa agtggagctg
     3781 gaccttctgc agatcgaacc agcacccccg aaagcatgat caggctgttt cccggtgttc
     3841 tccgggaggc ggcgcacttc aacataacga tagcctgacc tcggttgtga ggtggagggc
     3901 atcggaggta gcacgtggca aaaaaagtca tcgcgcagat caagttgcag ttgccggccg
     3961 gccaggctac tcctgcgccg ccggtggggc cggcgctttc gcagcatcgg ctcaacatca
     4021 tggagttcgt gaaagcgttc aacgaacgga cgcggtccca gcaggggatg atcattcctg
     4081 cagtcatcac cgcctacgcg gattccagtt tcacgttcat cctcaaaacg ccacccgcgg
     4141 tgactttgct gaaaaaggcc gccgggctgg aaaaaggttc acccgagccc aacgcgaaga
     4201 aggtgggcaa ggtcacgtgg gatcaggtgg tagaaatcgc gaagaccaag cttcctgatc
     4261 tcaacaccac aaaccttgat tccgccgcca gcatgattgc cggcactgcg cgcagcatgg
     4321 ggctggaagt tgttggaaga cccaccaagt agaggacata accatgccga aactggcgaa
     4381 gaaatatcag catgacctcg aaaaggtgga tcgcaccaag agttacgggt tgcgggaagc
     4441 aatcgaggta atcaagcagt ttgagccgcg cggtttcgac cagacggtcg aaggcgtgat
     4501 gaagttgaat gtcgatccgc ggaaggccga ccagctcgtt cgtggatcgg tcgttcttcc
     4561 cagtggaacg gggaaaacgg tgcgcgttct ggtgtttgca cgcggagagg ccgcgacggc
     4621 cgcggaacag gccggggcgg atttcgtggg tgcagatgac ctggttgcga agattcagga
     4681 ggggtggacg gatttcgatg tggcgattgc caccccggat ctgatgggtg tggtgggccg
     4741 tctggggcgc gtgcttggtc cccgtggcct tatgccgaac ccgaagacgg gcaccgtgac
     4801 acaggatacg gcagcagccg tgcaggccgc caaaggcggc aagatagatt tccgcgtgga
     4861 caaggcagga aacgtgcagg cgccgctggg acggttatcc ttcagcgtgg acgcgttgta
     4921 cgcgaacgcg caggtgttct ttgaggcagt gatgcgtgcg cggccgacgg cggtgaaggg
     4981 ccagtacgtt cactccgcca atctgtgtgc cagcatgacg cccagtgtgc cgctggacag
     5041 gaaccagttc cagtcgctcg tgaagtaatt cgttgaagaa ggttcaggcg atgccgaccc
     5101 cgaaaaagaa tgagacgata gcccgtttga ccgacatgtt caattcggcg aacgggatat
     5161 tcctgacgga ttccacgggt ttgaaggtgg aacttgtcac gaacctccgc aagcgttgcg
     5221 cggcggaaaa catcggcttc atggttgtga agaacacgct ggcgcggcgt gccgcgaagg
     5281 cagcgggttt gcccgacctt gacaagtggt tcaccggacc gactgccgtt gcgtattcga
     5341 agaacaaccc ggcaggtccg gtaaagattc tgagagcgtt tgttcgcgaa gttcgcgaag
     5401 caaacggcaa acttgagatc aaaaagggcg tggttgacgg tcaggtaatt gacgacgccc
     5461 agatgcagat gctggcagat cttccgacgc ctgaggtcgt tcgcgcgcga ttcctcgggc
     5521 ttctgcaagc tcccgccagt cagttcgttg gtatactgtc ggctggaccg gcttcgctgg
     5581 tgcgtgctct ggatcagcgc cgacaacaac ttgatgagca agcggccgga tcgacggccg
     5641 gttcaaccga agccccgacg gatgcacccg cagggcaata aggaggaaga gtctccatgg
     5701 cggatcttca agcgttgaaa acccagttga gcgcactgac ggttctcgaa attgcgcagt
     5761 tgacgaagga acttgaggct gagtggggtg tttcggctgc agcgcctgtt gctgttgccg
     5821 ctgcccctgc ggccggtggt gcggctgccg ctgcaccggt tgaagagcag acggaattca
     5881 ccccgacgtt ggtggatgta ggctcccaga agatccaggt gatcaaggtt gtgcgcgaac
     5941 tgaccggcct gggcttgaag gaagcgaagg cgctcgtgga tgccggcacc gctcccgtga
     6001 aggaaggtgt ctccaaggaa gaggcgaaca cgatcaaaga aaagctcgaa gcagtcggag
     6061 cgaaagtaga gatcaagtaa tctcccgtct cgagcttgtg caaaggcgac gcatcggctg
     6121 attctcggcc tcggcatgat gtgtcgtctt tgctgttctc atcagggcac ccgccacgaa
     6181 gggcaacgag ttctttgagg gaagggtgcc gcgactttgt tctttcgcca aaaggagcgg
     6241 attggggcca tgccagagcg gatgataaaa cgccggtcat atgccaagct ccccacaatt
     6301 ctcgatatcc ccaaccttct tgagttgcag gttgcctcgt ttgaggattt ccttcaggcc
     6361 aacactcctc cggagaagcg gaaaatcaag ggcttgcagg aggtgttcga gagcgtattc
     6421 ccgatcaccg atgttcacaa cctgtactcg cttgagtttg tcagctattc tatcggccgc
     6481 cccaaatacg acatcgacga atgccgtgac cggggcgcga cgtacgccgc accgctcagg
     6541 gcggttttgc ggcttgtcac gcgggaaaaa ggtgacgaga aacgggtcaa ggacgtcatt
     6601 gagcaggtag tttaccttgg cgatctgccg ctcatgacct ccacgggcac gttcctcatc
     6661 aacggtgctg agcgcgtaat cgtgagccag ttgcaccggt cgcccggcgt gttttttgat
     6721 gaggttgccc accccaacgg caagagactc tttaccgcgc ggatcatacc ctaccgtggt
     6781 tgctgggtcg agtttacgat ggacatcaat gatgtcatgt atgtccatat cgaccgcaaa
     6841 cggaagcagc cggtaacgtt gctgctgcgc gcgcttggat atagctcgga ccttgacgtg
     6901 ctcaatctgt tctatgaacg gatcacgacc aagctttcga gcaaggtgaa agggcggctc
     6961 ctggcggcgg acgtggtaaa tgccgcgacg ggagaggttc tggcggacgc gttcgccacc
     7021 cttaccgacg agacgatcgc gatgctcgag gaacacgggg tggaggaagt tgaagtctac
     7081 agtccgaaca ccgaggatga aatcggcatt atcgaaaaca cgttgcggcg tgacccctgc
     7141 caggacgaac gcggggaaga agacgccctt taccgcatct atcagttgat gcgtccgggc
     7201 gatccgccga acgtggaaac tgcgcgaacg ttcgtcgagc ggctcttttt cagtccgaaa
     7261 cggtacgatc tgggagatgt cggccggtac cggatgaatc agaagctcga tctcaagatt
     7321 cctcaggaca cgcatgtcct gacgcgtgag gacttcatct ccatcatccg gtatatcatc
     7381 gcgctgcgga acggatcacc aagcgcgcag accgatgata tcgaccatct cgggaatcgt
     7441 cggtgccgga cggtgggcga gctgcttgcg aaccagttct cgatcgggct ttcgcgcatg
     7501 gctcgcacca tccgcgagcg gatgagcctc cgggacaatg agaatctgac gccgcaggaa
     7561 ctggtcaacg cgaaaaccgt ttcgacggtg attgcgtcct ttttcggttc cagccagctc
     7621 agccagttca tggctcagac gaaccctctg gacgagctta cgcacaagcg gcggctgagt
     7681 gctctcgggc cgggtggtct tacgcgggag cgcgccgggt tcgaggtccg tgacgttcac
     7741 cacactcatt acggccgtgt gtgccctatc gagacgccgg aaggccagaa tataggtctc
     7801 atcatgtcgc tcgcggcgca tgcccggatc aaccagtacg gattcattga gacgccgtac
     7861 cgggtggtga aagacggtgt ggttacgacc gaaatccggt atctgtcttc ggatgaggaa
     7921 gaccggtacg tgatcgcgca ggcgaacgcg cccttgaagg aagacgggtc ctttgccaac
     7981 aacctggtga aggcgcgtcg gcgcggagat ttccccacgg tcagtcccct ggaagtggac
     8041 tacatggatg tgtcgccgat tcagcttgtc agcgcggcgg cggcgcttat tcccttcctc
     8101 gagcatgacg atgccaaccg tgcgctgatg ggttccaaca tgcagcggca ggccgttccg
     8161 cttctgaaag cggaggcgcc gcttgtcggg acgggaatcg agcggcgtat cgcactcgat
     8221 tccggcgcgg tgacgcttgc gaaagccgac ggcgaggtgg tgtacgtgga ctcgtcgaag
     8281 atcgtcgtgc gcgaaaagaa gccggttcac gccaatcctc tggcgctgga cgaaggtcac
     8341 gagcgcaaat acagcctcat gaagttccag cggtcgaacc aggattgctg ccagaaccag
     8401 cgtccacttg tgcggcccgg ggacaaggtg aaggcgggtg aggtgctcgc ggacggccct
     8461 gcgacggaca gaggtgacct ggcgctgggg tgcaacgttc tgtgcgcgtt catgccgtgg
     8521 cacggctaca attttgagga cgccatcgtg gtgagcgagc ggctcatcag aaacgacgtc
     8581 ttcacgtctg tgcacatcga ggaatacgag cttcaggtcc gagacacaaa acgtggccag
     8641 gaagagctca cgcgggatat tccgaacgtg agtgaccagg cgctgcgtga cctcgatgag
     8701 aacgggattg tccgggttgg cgcgagagtg aagcccggag acatcctggt cgggaaggtg
     8761 acgccgaaag gcgaaacgga actctcgccc gaggagcgcc ttctgcgggc gattttcggg
     8821 gagaaggccg gtgacgtgcg ggatgcgtcg ctgaaggccc cggccggcat ggaaggcgtc
     8881 gtcattgata cgaagatatt ctcccgcaaa gagcgcgatg aggccacgcg aagccgcaac
     8941 aaggaaacga tcgagcaaat acgcgcgaac atggatgagc gcgccgcacg agttcggagc
     9001 gctttgaagc agcggtccat tgcggtctgc caggggttga cggcaggcaa ctggcgctcc
     9061 gaggcagacg gaacgatcgt ggttcacgaa gggaccgtcc tgaccgagga gatcctttca
     9121 gaactcgatc tgacgactct tcgacctgat acgacgtgga cgactgactc ggcggccaac
     9181 ggcaagatcg aggaaatggt tcggtatgcg cacgacatgc ttgcggcgat tcaggaggaa
     9241 tttgatcgcg agagcgagaa ggtggtgcgc ggagacgagc ttccgccggg cgtgattcaa
     9301 cttgtcaagg tgtatgtcgc gcacaagcgc aagctctccg tcggggacaa aatggcggga
     9361 cggcacggca acaagggtgt tgttgcaaag gtggttccgg tagaggacat gccgtatctg
     9421 ccggatggca ccccggtgga tctggttctc aatcccctcg gcgtgccctc gcggatgaat
     9481 ctcgggcaga ttctcgagac ccatctcggc tgggctgcgg agaaactggg aattcacgtg
     9541 tcgtgcccgg tgtttgatgg cgcgaccgtt gcggaaatca agaatgcgct gaaagaagct
     9601 ggtttgccgg aatcggggca gacggttctg tacgacggta tgacgggcga gagattcgag
     9661 cgcgaggtga cggtaggtta catctacatg atgaagctga tccaccttgt tgcggacaag
     9721 atccacgctc gttccatcgg cccgtacagc ctcgtcacgc agcagccgct cggcggccgg
     9781 gcccagtttg gcggccagcg ttttggagaa atggaagtgt gggcgcttga agcatacggc
     9841 gcgtcccatc tgcttcagga gatgctgacg gtgaagtcgg acgacgtccc gggacggtcg
     9901 cggatatacg aagcgatcgt caagggcgag aatccgccgg agccgggcat cccggaatcg
     9961 ttcaacgtgc tcgtgaaaga gcttcaggca ctgtgccttg acgtcgtgct ccagcaagac
    10021 ggtcaagtgg ccgaccagag ctgaaacctc taccctaacc ctcgtaacga gaggagaacg
    10081 gccgtggtcg aacaaccgga cgttttggcg tcgcaactgc tgtcgcgcgc aggtttgccg
    10141 cagcagcgca agccactcga tttcgattcg ataaagatac aactcgcttc accggagacg
    10201 attcggagct ggtcgtacgg tgaggttctg aagcccgaaa cgatcaacta tcgcacgtac
    10261 aagcccgagc gcgatgggct tttctgcgag cggattttcg gccccacgcg tgactgggaa
    10321 tgccactgcg ggaagtacaa gcgcatccgg tacaagggtg tgatctgcga ccggtgcggc
    10381 gttgaggtta cgcaggccaa ggttcggcgt gagcgcatgg gacacattga gcttgccgtg
    10441 tctgtgtcac acatatggta cttcaagtcg gttccgtccc gtatgagcta tttgctcgcg
    10501 atgacggtcc ggaatctgga acgcgtgctg tactatgagt cttacgttgt cctggatccc
    10561 ggaaatgcgc ctctggagaa gggtgatctg atctcggaag agcaggtcat tgaccttgag
    10621 gaacgcggtt ttcagttctc ggcggagatg ggcgccggcg ccatcaagaa gatgctggcg
    10681 gatttgaacc tcgatgagct ctcgacggag cttcgggaga cgattcgcaa ggaaacgagc
    10741 gcccagcgga aggctgaggc cctccgccgc ctgagcatcg tggaggcatt ccgccagtcg
    10801 aacaaccggc ccgagtggat gattctggat gtgataccgg tgcttccgcc ggaattgcgt
    10861 ccgcttgttc cgcttgaagg aggccgtttt gcgacgagcg acctgaacga tctctatcgt
    10921 cgggtgatca accgtaacaa ccggttgaag aagctcatag aaatcaaggc accggaagtg
    10981 attctccgca acgaaaagcg gatgcttcag gaagccgttg atgcgctgtt tgacaacggg
    11041 cggcggagcc gcgcggtgcg tggcgacgcg aaccgggcac tgaagagcct gggcgacctg
    11101 ttgaaaggca agcaaggccg attccggcag aaccttctgg ggaagcgtgt tgattactcg
    11161 ggtcggtcag tcatcgtggt gggcccggaa ctgaagcttt atgagtgcgg gttgccgaaa
    11221 atcatggcgc tggagctttt caagccgttc atcatacacg agcttgaacg gcgcgggctg
    11281 gtgcagacgg tcaagagcgc gaagaagaag atcgaacagg gcagcactga agtgtgggac
    11341 atccttgagg aagtgatcaa gggacacccg gtgctcctga accgcgctcc tacgcttcac
    11401 cggctcggta ttcaggcgtt tcagcccgtg ctcgtggaag gcaaggccat ccggattcat
    11461 ccgctggtct gcaaagcgtt caatgccgac tttgacggcg accagatggc agtgcacgtg
    11521 ccgctctcct tcgaggcgca gatcgaagcc tcggtgctga tgctttcggc gaacaatctg
    11581 ctgctgccgt cgagcgggaa cccggt
//
LOCUS       DAOSOO010000091        11556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5564_length_11556_cov_5.429615, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000091 DAOSOO010000000
VERSION     DAOSOO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5564_length_11556_cov_5.429615"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLT86_07905"
     CDS             <1..668
                     /locus_tag="PLT86_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOT36573.1"
                     /translation="ISTQHKPGSMHILGYFIDVGEHRLQNTLAEMVRNRDRRNRGIVE
                     RLNNLGVQMTLEEVAEYAGGDVLGRPHFARALVEKGVVSTIQEAFDHYLDAGAAAAVP
                     KEKLPPELVIDMIHGAGGLAVLAHPYQLRLGDGEELEKRVRLLCDQGLDGIECFYPEH
                     SEPQTAIYLSLAARYSLVATGGTDFHGSSKNQTRLGLQPPLRASCVLQLAARRARNIC
                     PRS"
     gene            710..1237
                     /locus_tag="PLT86_07910"
     CDS             710..1237
                     /locus_tag="PLT86_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36574.1"
                     /translation="MAVPAVKIERHSSGAWRITCWPRDRVRKVAFALGGLVAGLLMAV
                     VSVSGLLSALAGEQNGAELFVLGWLGPLAVISALLIWLAVSLVFRAVQGFEVECDGTV
                     VSVVRFSPHGSRELMRCPITECELVSGGTDLAFRFARPGSDKKEVLDTHCRLSPEEHL
                     AIERLFLIREPSGNA"
     gene            1328..2170
                     /locus_tag="PLT86_07915"
     CDS             1328..2170
                     /locus_tag="PLT86_07915"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOT36575.1"
                     /translation="MQSAIALEGVSFSYGENEVLSAVTFALGSGEFVGIIGPNGSGKS
                     TLLRVMAGILRPSSGVVTMAGRPMQNLSRREIARSVAVVPQETTFVFPFTALEVVLMG
                     RSPHLGRFGIEGEDDLEVARQAMEATDTWFLRDRTMEEVSGGEKQRVIVARALAQKPE
                     ILLLDEPTNALDIRHQVDIYQLLQRLHDESGLTIVLISHDLNLSARACERLILLHRGR
                     IQCDGGPDEVITSALLSEVYETELEVHTDPATGAPIITTPIDRVVRKKSPPFRAGEKQ
                     RKIS"
     gene            2287..2625
                     /locus_tag="PLT86_07920"
     CDS             2287..2625
                     /locus_tag="PLT86_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36576.1"
                     /translation="MESEVIAEPVAADALAAMRKVEDEAEALVAHARAEAKELLERAR
                     REAEERRSREHALMQREMELRRQELLNGVLRQTEALKADAEAEKASYAAEVRRITPQL
                     VEEIVALVVG"
     gene            2675..3685
                     /locus_tag="PLT86_07925"
     CDS             2675..3685
                     /locus_tag="PLT86_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOT36577.1"
                     /translation="MASASAFLNARAHALLGMLLPRETLLRLADLPSAEAILADLSAR
                     EYFRASLPAEQLTFPQRLNLELDADYRSTLLVLPQESRPLVRALYRLREVAFLKAALG
                     CANRNVPPASRGAYLGGLHLFNIADEEAFCQAADVAEAVSKAPEPYRTPMENALHQVE
                     EQGSLFPIEIALDLLVLHELWLAGRALTGMDRARIFPMFGVWFDVFNVLSAARLRLNF
                     AVPSDEALGYLIHHGQFLRLSHRRALAGLEKFSDLSAALRDTPYSAKLNGAESLFAVE
                     QGMMRAFREVISASLAGYPFHLGGTVAYLLLKQIEITNLTRVYEGKRRDVKTAGWQDV
                     LV"
     gene            3700..5658
                     /locus_tag="PLT86_07930"
     CDS             3700..5658
                     /locus_tag="PLT86_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOT36578.1"
                     /translation="MFRPIPMARIGVIVRDDQSTQLTRLLAAEGVLQIESPSTQLGLD
                     TATARSSISRWEALERQVTELAQNLEIRLSSDKSPFEVVVDPKDDLEPCEKRVAEILS
                     QAEQIRGDLAAIATKRTKTLGQIEELLLLSPVNWTLEELRSTQQLAVHIGVLSEVNVR
                     RVEAILGALPHTTVRLPGPATGWDVVIVFSQKTTADQVERALSSANFKPLEIPEDLTG
                     KPGEAEEKLWKLLEELEEQRKTLTQQLRETTTAEMGELCEIWEKARAARTLLAAQQPF
                     GKTRALSYVTGWVPKESSQALTQRIRDTIGAAAYVEVRDPDPHDPAVPVRLKNPSVFR
                     PFEEVVTTYSLPSSKEIDPTVVVAITYLIMFGMMFGDVGQGLVLAGAGFWAAKKAPSV
                     KNLGLILGMCGISSVIFGFIYGSVFGVEMQLNPWINLPLVEHHTSVMASNLAVLGGIS
                     IGFGIIMLSLGIVFNIVNAVRAKEWGIAVFDQYGLTGLAFYWCALIWAVLTALGYAGL
                     GMTALAAGALVALVVLFVREPIIRATERKEHLVEGGVGFFVVQALIDTMDMVIRFLSN
                     TVSFIRVAAFGLTHAALFMAIHTIGFMISGSTHGAGYIITFIVGNIFVVVMEGLIVSI
                     QCLRLQYYEVFGKFFRGGGLPFAPLRAS"
     gene            5699..6049
                     /locus_tag="PLT86_07935"
     CDS             5699..6049
                     /locus_tag="PLT86_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOT36579.1"
                     /translation="MKTLKVVLGALLMLNLAVILTAAAVVLFPDLALAAEGAPAAAAG
                     SDRGLMMIGAALAVGLGCVGAGLAVAQVGAAALGAIGEKPDMLGKTLIFVGLAEGIAI
                     YGLIISIMIYASAG"
     gene            6075..6386
                     /locus_tag="PLT86_07940"
     CDS             6075..6386
                     /locus_tag="PLT86_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOT36580.1"
                     /translation="MKVYCIGSEEVVKGLALIGIEGTATTDAQEAERAVDAVLNQPEV
                     GLLVISERLAMPLKEKLTGWAFAHRQPLILEIPDEQGPVSKHSAVLSLVMEAVGLEQS
                     S"
     gene            6383..6997
                     /locus_tag="PLT86_07945"
     CDS             6383..6997
                     /locus_tag="PLT86_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOT36581.1"
                     /translation="MNNPIDSLREAVIRDAQAQAYEIMQDAIAAGQRLAAEMDARIAQ
                     DRAEALAKAEQKALQEQQRLLASAQGKIQKERLLEREKRIAEVIEAARSALAEYARQP
                     GVGRDLLLKLCVEGATALRSEKVLLVVRPDQRSWVDTPFLLAVQQQSGKRVELSDENL
                     TAIGGVIVTSSDGRERFDNTLDNRLARVIDDVRAMIWTRISHAR"
     gene            6987..8759
                     /locus_tag="PLT86_07950"
     CDS             6987..8759
                     /locus_tag="PLT86_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOT36582.1"
                     /translation="MHDEPVGKVSWVSGPVVRTMETRGVHMLDMVEVGDERLIGEIIG
                     LNGDQATLQVYEDTTGLKPGSPVYSTGYPLAAELGPGLVTSIYDGILRPLKALADATG
                     IFIGRGVRAPSLSREILWDFTPVVRNGQEVTPGMIIGTVPETERIEHRVMVPPNVSGI
                     VTSVASAGKYKVDQEICRVRTPQGDEVPLMMLQRWPVRQKRPVTRRLPADTPLVTGQR
                     VVDCVFPMVKGGAAAIPGGFGTGKTITQHQLAKWSDADLIVYIGCGERGNEMTEVLVE
                     FPKLIDPHSNRPLMERTILIANTSDMPVAAREASIYTGITLAEYYRDMGYHVALMADS
                     TSRWAEALRELSGRLEEMPAEEGFPAYLATRLAEFYERAGMVETLGGSTGSVSVIGAV
                     SPAGGDFSEPVTQHTSRFIGCFWALDKELASARYYPSINYINSHSAYTEFVADWWKKI
                     GGEKYNWEAMREETVDVLRRDDKLQQIVKLVGEEELPDEQKLILDTARILQNAFLQQS
                     AFDEVDTYSVPEKQLRMLSVILHYYHRGSDVVKRSIPIYKYQQLPVRNEIMRMKSRIP
                     NDKLEEFDTLERAIDEQIDALIAE"
     gene            8770..10155
                     /locus_tag="PLT86_07955"
     CDS             8770..10155
                     /locus_tag="PLT86_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOT36583.1"
                     /translation="MATEIGKEYVGVQRIVGPLVMIEGIDGVGYDEMVEIVSDDGKLK
                     HGRVLEVGTKAAVVQVFEHTTGLSIGATKMKFLARPVRIPVSEEMLGRTFNALAEPID
                     GGPPPIPKDLRPVSGAAINPTLRHHPSDFIQTGISAIDAMSTLVRGQKLPVFSASGLE
                     HDRVIAQIIRQAKIIGKQEPFAVVFGAMGLKHDIADFYRKSFEESGAGNNVATFLTLA
                     DDPSIERIITPRSALTLAEYLAFDVGMHVLVVLTDMTNYCEALREVATAKGEVPSRKG
                     YPAYLYSDLAALYERAGQVEGRTGSITQLPLLTMPNEDITHPIPDLTGYITEGQIVLS
                     KALQGAGIYPPINVLPSLSRLMNDGIGEGRTREDHRNVSSQLYAAYARVKRVEVLAAV
                     IGEEELSEIDKQYLTFGQHFEKEFIQQAPDEDRSIEETLNLGWKLLKYLPVSELTRVK
                     EEQIAKYLPKD"
     gene            10228..10854
                     /locus_tag="PLT86_07960"
     CDS             10228..10854
                     /locus_tag="PLT86_07960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOT36584.1"
                     /translation="MPRLNIPATKINLIRIKDELKLAVDGHELLEQKRQVLIMEVRKL
                     INSLRAERSEMENLLREAYAKLAQATWENGAEQVALAGLATSQAIDISLRERSVMGII
                     LPVVDATEAELRPSYSLSGTTSSLDEAARAFHGALQHIARLAELENAALRLATELMKT
                     QRRVNALKNFFIPQYHETVKFLQETLEERERDSLFALKRLKSKADLPA"
     gene            10983..>11556
                     /locus_tag="PLT86_07965"
     CDS             10983..>11556
                     /locus_tag="PLT86_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT36585.1"
                     /translation="MVSPLPVLEDKTLFARGDTSITPAPEEISRLVIYLVKPSRYDDD
                     GYLVRHWRGVLPSNTLSCLAGLTDDVVQRKVLGEHLKISTVLVDESVERMPRRKILAD
                     AGRKGTRAVVCLAGVQTNQFARATDIALEYASLGVRVMIGGFHVSGMTAMFPEGTPDI
                     ARLTDAGVTVVAGEVEDRWAALLSDVVNDRL"
BASE COUNT         2463 a         3080 c         3594 g         2419 t
ORIGIN      
        1 agatcagcac gcagcacaaa ccgggaagca tgcatattct cggctacttc atcgatgtcg
       61 gtgaacaccg acttcaaaat acactggcgg aaatggtacg caatcgcgac cgaagaaatc
      121 gtggcatcgt cgagcgtctc aacaacctag gggttcagat gacgctggag gaagttgctg
      181 aatatgcggg tggcgacgtt ctgggaaggc cccatttcgc gcgagcactg gttgagaagg
      241 gggttgtctc gacaattcag gaagctttcg accactatct ggatgccgga gctgcagccg
      301 cagttcccaa ggaaaaactt ccgcctgagc ttgtgattga catgattcac ggggcgggcg
      361 gtctggcggt gcttgctcat ccgtatcaac ttcggctggg tgacggcgag gaactcgaga
      421 agcgggtacg gttgctttgc gatcaagggc tcgacggaat cgagtgcttt tacccagagc
      481 attctgaacc acagaccgcg atttaccttt ctctggccgc ccgttacagt ctcgtggcaa
      541 cgggcgggac tgattttcat ggatcgagca aaaatcagac ccgcctcggg ttgcagccgc
      601 cccttcgggc ttcgtgcgtt ctgcagttgg ccgcgagacg cgcccggaac atttgtccgc
      661 gttcttgaag attcgacgag aacttctcct ggatcggaac gaaagacaga tggcagttcc
      721 agcagtgaaa attgagcggc attccagcgg cgcgtggcgc ataacctgct ggccacgcga
      781 cagggtgagg aaggtagcat ttgcactggg tggtttggtg gccggtttgt tgatggctgt
      841 tgtgagtgtt tccggcctgc tttccgcact tgcaggggag caaaacggcg cggaattgtt
      901 cgtactggga tggctcgggc ccctcgccgt gatttcggcg ttgctgatct ggcttgccgt
      961 ttcccttgtg ttccgcgcag tgcaagggtt tgaggttgag tgcgacggta cggtcgtatc
     1021 cgttgtgcgt ttttcgcccc acggcagcag ggaattgatg cgctgtccga taacagaatg
     1081 cgaactcgtg agcggcggca cggaccttgc ctttcggttt gccaggccgg gaagcgacaa
     1141 aaaggaagtg ctggataccc attgccgact cagccccgaa gagcatctcg caattgaacg
     1201 actttttctt attcgcgagc cttccggaaa tgcgtgaaaa gcgaactgga tcctcacgcg
     1261 tgctcaattg tccgtgcaca gcgtctccac atgtgtctga gcggccgcct cgatgaacga
     1321 atccaaaatg caatctgcga tcgccctgga gggtgtctcg ttctcatacg gcgagaacga
     1381 agtcctttcc gctgtcacat tcgccctggg gtccggcgaa tttgtgggaa tcatcggccc
     1441 gaatggctcg ggtaagtcca cgcttctccg cgtcatggcg ggtatcctgc gcccttcgtc
     1501 tggagtcgtg acgatggcgg gacgccccat gcagaacctc agccgccggg aaatcgcccg
     1561 cagtgtagcc gttgtgcctc aggagacgac ctttgtgttt ccatttaccg cactcgaagt
     1621 ggtgttgatg gggcgctcac cccatcttgg ccgtttcggg attgagggcg aggacgatct
     1681 ggaagtggcg cgacaggcca tggaggctac cgacacctgg ttccttcgag atcggacgat
     1741 ggaggaagtg tcggggggcg agaaacagcg tgtcatcgtc gcgcgtgccc tggctcagaa
     1801 gccggaaatc ctgctcctgg atgagcccac aaacgctctt gacatccggc atcaggttga
     1861 catttaccag ctcttgcagc gattgcacga tgagtccggt ctcacgatcg tgctgatttc
     1921 ccatgacctg aacctgtcgg cacgagcgtg tgagcgtctt atcctcctgc accgggggcg
     1981 catccagtgc gacgggggtc ccgacgaagt gatcacgagc gctcttctga gcgaggtcta
     2041 cgaaacggag ctcgaggtac acactgatcc tgctaccggt gctccaatca tcacgactcc
     2101 gattgaccgg gttgtgagga aaaaaagtcc gccgttccgc gcgggagaga agcaacgaaa
     2161 aatatcctga ggaggttacg ctttttgctt gcttcgagta cgtcatgctg ctattattgg
     2221 aaccatgtgg aacatgcaaa cgtgagggat ttccaacgcg gcgcgcttct gagcaagggg
     2281 tgagatatgg agtccgaagt tattgccgaa ccggttgcgg ctgatgctct tgctgcgatg
     2341 cggaaggtag aggacgaagc cgaagcgtta gttgcgcatg cacgggccga ggcaaaggag
     2401 cttctggagc gtgcgcgtcg cgaagcggaa gagagacgtt cccgggagca cgctctcatg
     2461 cagcgcgaga tggagctcag acggcaggag cttctgaacg gtgttcttcg tcagacggaa
     2521 gcattgaaag ccgacgctga agctgaaaaa gcaagctacg ccgctgaagt gcgccgcata
     2581 actccgcagc ttgtcgagga aatcgtcgct ctcgtcgttg ggtaacaccg cgtcagatgc
     2641 cggtgtgatt ccggtccttt tgggggaacg gggcatggcc tcagcctcgg cgtttttgaa
     2701 tgcacgcgcg catgcccttc tcggcatgtt gctgccgcgg gaaacccttc tgcgacttgc
     2761 tgatcttccc agcgcagaag cgattctggc agacctttcg gcgcgcgagt actttcgggc
     2821 ttcgctgccg gcggagcaac tgactttccc ccagcgtttg aacctcgaac tcgacgcgga
     2881 ttaccgatcc accctcctcg tgctgccgca ggaaagccgg cctttggtac gggcgctata
     2941 tcgtttgagg gaagtggctt tcctcaaagc cgcgcttggt tgcgcgaatc ggaacgtccc
     3001 gccagcctca cgcggcgcat acctcggggg gcttcatctt ttcaacattg cggacgaaga
     3061 agctttttgt caggcagctg atgttgcgga agctgtctcg aaggcgccgg agccgtaccg
     3121 aacaccgatg gaaaacgcac tccatcaggt cgaggagcaa gggtctctct tcccgatcga
     3181 aattgccctc gaccttctcg tgctccacga gctgtggctc gccggccggg cattgacggg
     3241 aatggaccgg gcgcggattt tcccgatgtt cggggtatgg tttgacgtgt tcaatgtgct
     3301 gtccgccgcg aggttgcggc tgaatttcgc ggtgccgagt gatgaagcac ttgggtatct
     3361 cattcaccac gggcagttct tgaggctttc acatcggcga gcgctggccg gtctcgagaa
     3421 attcagtgat ctttctgcgg cgttgcgtga tacgccgtat tccgcgaaac tcaatggcgc
     3481 ggaatcgctt tttgcagtgg aacaggggat gatgcgggcg tttcgcgagg tgatctcggc
     3541 aagtctggca ggatacccgt tccacctggg cggcacagtt gcatatttgc tgctcaaaca
     3601 gatcgagatc acgaatctga cacgcgtgta cgaaggcaag cggcgcgatg tgaagaccgc
     3661 cggctggcag gatgttctcg tgtaggaagg ggttccgtcg tgttccgacc gatcccgatg
     3721 gcacgaatcg gcgtgatcgt gcgagatgat caatccacgc agttgactcg cctgcttgcg
     3781 gccgaagggg tgctgcaaat cgaatctcct tcgacccaac tcgggctgga taccgcgact
     3841 gcgcgttcgt ctatatcccg ctgggaggcc ctggaacgcc aggtaacaga actggcgcaa
     3901 aacctggaaa tccgcctttc atccgacaaa tcaccgttcg aagtggtggt ggacccgaaa
     3961 gacgatctcg aaccctgcga gaaaagagtg gcggaaattc tttcgcaggc ggaacagata
     4021 cggggcgatc tggcagcaat tgcgacgaaa cgaacaaaga cgctcgggca gatcgaagag
     4081 cttctgcttc tttcaccggt caattggacg ctggaagaat tgaggtcaac ccagcaactg
     4141 gcagtccaca tcggtgttct cagcgaggtg aacgttcggc gcgtagaagc gatcctcgga
     4201 gctttgccgc acaccacggt ccgattgccg ggtccggcga cgggatggga cgtggtgatc
     4261 gtcttctccc agaaaactac ggcggaccag gtggaaaggg ctctttcaag cgcgaacttc
     4321 aagccgctgg aaattcccga ggatttgacg ggcaaaccgg gcgaagcgga agagaaactc
     4381 tggaagctgc tggaggaact tgaagagcaa cgaaaaacgc tgacgcaaca gctccgagaa
     4441 acgacgactg ctgagatggg ggagctgtgt gagatctggg agaaggcccg cgccgcgaga
     4501 acccttctgg cggcgcagca gccgttcgga aaaacgcgag ccctttcgta cgtcaccggt
     4561 tgggtgccga aagaatccag tcaggcactg acacaacgca tccgtgatac cattggcgct
     4621 gctgcatacg ttgaagttcg tgatccggac ccacatgacc ccgcggtacc ggtgaggctc
     4681 aaaaacccgt ccgtgttcag gccgtttgaa gaggtagtga caacctactc ccttccttcg
     4741 tcgaaagaaa tcgatccgac cgtagttgtg gcgattacct acctcatcat gttcggcatg
     4801 atgtttggag acgtgggaca agggttggta ctggcaggtg cggggttctg ggccgcgaaa
     4861 aaagcgccat cggtgaagaa tctcggtctc attcttggca tgtgcggcat ttcttcagtg
     4921 atcttcggtt tcatatacgg gagcgttttt ggtgtagaaa tgcagctgaa cccgtggatc
     4981 aatttgcccc ttgtggaaca ccacacttcg gtgatggcgt ctaatctcgc tgttctcggg
     5041 ggcatttcca tcggtttcgg aatcatcatg ctctctctgg gaattgtttt caacatcgtc
     5101 aacgccgtgc gggcgaagga atgggggatc gctgttttcg accagtacgg gttgaccggt
     5161 ctggcgtttt actggtgtgc tctcatctgg gccgttttga cggctctagg ctatgcgggt
     5221 ttgggtatga cggcactcgc agcgggggcg ctcgtggctc ttgtggtgct gttcgttcgc
     5281 gagccgatta tccgcgcaac ggagcggaaa gagcacctgg ttgaaggtgg agtcggcttt
     5341 tttgtcgtcc aggcgctaat tgacacaatg gacatggtga tccgcttcct cagcaatacc
     5401 gtctcgttca tccgtgtcgc ggcgttcggg ttgacccatg cggcgctttt catggcgatt
     5461 cacacgatag gtttcatgat ctcgggttct actcacggag ccggttatat catcacgttc
     5521 atcgtgggca atatcttcgt tgtcgttatg gaaggcctga ttgtttcgat tcagtgcctg
     5581 cgtttgcagt attacgaggt gttcgggaag tttttccggg gcgggggtct gccgttcgca
     5641 ccgcttcgcg ccagttgagg ttcgctcata tcacttcaat acgaggggag tgtggttcat
     5701 gaagactctg aaggtcgtgc tcggtgcact gctgatgctg aacctcgccg tgatactcac
     5761 ggcggccgcg gtggtgctgt tcccagatct cgcgcttgct gcggaaggcg ctcccgcagc
     5821 ggcagcaggg agtgatcgag gtctcatgat gatcggcgcg gctttggcgg ttggtctggg
     5881 gtgcgtgggt gcgggccttg cggtagcgca ggttggcgcg gccgctctgg gcgcgatcgg
     5941 cgaaaaaccg gacatgctcg gcaaaacgct gatcttcgtc ggtcttgccg aaggaatagc
     6001 gatttacggt ctgatcatct ctatcatgat ctacgcttcg gcaggttgat cggtcgacgc
     6061 tccgagaggg tgtgatgaaa gtctattgca ttggcagcga ggaagtggta aaagggctcg
     6121 cgctgatcgg cattgaaggc accgccacga ccgatgccca ggaagcggaa cgggcagtgg
     6181 acgcggtgct gaatcagccg gaggtcgggc tcctcgttat cagtgaacgg cttgccatgc
     6241 cccttaagga aaagctgacc gggtgggcat ttgcccatcg ccaacctctc attctcgaga
     6301 tacccgacga gcagggcccg gtatcaaaac actcggctgt tctttccctc gtcatggaag
     6361 cggtcggtct ggagcagtcg tcatgaacaa cccaattgac agcctccggg aagcagtgat
     6421 ccgtgatgct caggcgcagg catacgagat tatgcaggac gccatcgcag cgggtcagcg
     6481 tcttgccgct gagatggacg cccggatcgc acaggaccgt gcagaggccc ttgcgaaggc
     6541 ggagcaaaag gcattgcagg agcaacagcg tcttctggcg agcgcgcagg gaaaaatcca
     6601 gaaagaacgc ctgctcgaac gggagaaacg catcgccgag gttatcgagg cggcacgaag
     6661 tgcgctggcg gaatacgcca ggcaaccggg cgtcggccgg gacctgcttc tgaaactctg
     6721 tgtcgaaggc gcgacggcgt tgagaagcga gaaggttctt cttgttgttc gcccggacca
     6781 gcgtagctgg gtcgacacgc catttcttct ggccgtgcaa cagcaatcgg gaaaaagagt
     6841 ggagctttcg gacgagaatc tcacggcaat aggcggagtg atcgttacga gttcagatgg
     6901 tcgcgagagg ttcgataaca cactggacaa tcgtcttgcg cgcgtgattg acgacgtgcg
     6961 ggcgatgata tggacgagga taagccatgc acgatgagcc tgtcggcaaa gtgagctggg
     7021 tcagtggacc cgttgtccgc accatggaga cgcgcggcgt ccacatgttg gacatggtgg
     7081 aagtcggcga cgaacggctc atcggcgaga tcatcggcct gaatggtgat caagctacac
     7141 tccaggtgta cgaggacact accggcttga agccgggatc tccggtgtac agcaccggtt
     7201 accctcttgc tgcggagctg gggccggggc tggtaaccag catttacgac ggcattctgc
     7261 gcccgctgaa ggcactcgct gatgcaaccg ggatatttat cggccgcggt gttcgcgctc
     7321 cttcactttc gcgtgagatt ctgtgggatt tcactcccgt cgttcgaaac gggcaggagg
     7381 ttacccccgg catgatcatc ggaacggttc cggaaacgga acggatcgag caccgggtga
     7441 tggttcctcc gaacgtttcc gggatcgtga cgagcgtagc atcggcgggc aaatacaaag
     7501 tggatcagga gatttgccgc gtccgcacgc cacagggcga cgaagtgccg ttgatgatgc
     7561 tgcaacgatg gccggttcgc cagaagaggc cggtgacccg ccggctgccg gctgatacgc
     7621 cgctcgtgac gggacaacgc gtggttgact gcgtcttccc catggtcaaa ggtggcgccg
     7681 ccgcaattcc gggcggtttt ggaaccggaa agacgatcac gcagcaccag cttgcaaagt
     7741 ggtcggacgc cgacctcatc gtgtatatcg gatgtggcga acgtggcaac gagatgaccg
     7801 aggtgctggt cgaattcccg aagctaatcg atccgcacag caaccggccg ttgatggagc
     7861 ggacgatcct gatcgcgaac acctccgaca tgcccgtggc cgcacgtgag gcgagcattt
     7921 acaccgggat cacgcttgcg gagtattacc gggacatggg ctatcacgtt gcgctcatgg
     7981 ccgattcaac gtcccgatgg gctgaagcgc tgcgtgagct gtccgggcgg ctggaagaaa
     8041 tgcctgcaga ggaggggttc ccggcatacc tggcgacaag actggcggag ttctacgagc
     8101 gtgctggaat ggtcgagacg ctcgggggaa gcacgggatc tgtgagtgtg atcggtgctg
     8161 tgtcgcccgc gggtggagat ttcagcgaac cggtaaccca gcatacgtcc cgtttcatcg
     8221 gttgcttctg ggcgctggac aaggaactgg caagcgcgcg gtactatcca tcaatcaatt
     8281 acatcaacag ccacagtgcg tacacggaat ttgttgctga ctggtggaag aagatcggtg
     8341 gggagaagta caactgggag gcaatgcggg aagagacggt ggatgtcctg cgtcgcgatg
     8401 acaaattgca gcagatcgtg aaacttgtcg gtgaagaaga actgccggat gaacagaaac
     8461 tcatcctcga caccgcgcgg atacttcaaa atgcgttcct gcaacagagc gcgtttgacg
     8521 aggtggatac gtactccgtt ccggagaagc aacttcggat gctgagcgtc atcctgcatt
     8581 actaccatcg tggaagcgat gtcgtcaagc gaagcatccc gatctacaaa taccagcagc
     8641 ttccggtgcg gaacgaaata atgcgcatga aatcgcggat tccgaacgac aagctggagg
     8701 aattcgatac gctcgaacga gcgatagacg aacagattga cgcacttatc gccgaatgag
     8761 gccacaccca tggcgacaga aattggcaag gaatacgtcg gcgtacagcg catagttggc
     8821 cctctggtca tgatcgaggg tatcgacggc gtcgggtacg atgagatggt ggagattgtc
     8881 tcggacgacg gcaagttgaa acacgggcgc gtgctcgaag tcggcaccaa ggccgccgtc
     8941 gtacaggtat ttgaacacac gacgggcctc tccatcggcg cgacgaagat gaaattcctc
     9001 gcccgcccgg tccgaatacc ggtttccgag gaaatgctcg ggcgcacatt caacgcgctg
     9061 gcggagccga ttgacggcgg gccgccaccg attccgaaag atttgcgccc ggtttccggc
     9121 gcagcaatca atccgacgct ccgtcaccac ccgagcgact tcatacaaac cggtatctct
     9181 gccatcgacg caatgagcac gctcgtgcgt gggcagaagc tgcccgtatt ctcagcgtcg
     9241 ggactggaac acgatcgggt gattgcccag atcattcgtc aggcgaaaat catcggcaaa
     9301 caggagccgt ttgccgtggt gtttggcgcg atgggtctga agcacgacat tgcggatttc
     9361 taccgcaaga gctttgagga gagcggagcg ggaaacaacg tcgccacatt tctgacactc
     9421 gcggatgatc cctcaatcga gcggatcatt acgccccgat ccgcgctgac gcttgcggaa
     9481 tacctcgctt ttgacgtcgg tatgcacgtc ctggtggttc ttaccgacat gacgaattac
     9541 tgcgaagccc tgcgggaggt ggcaacggca aaaggggaag tgcccagtcg aaaagggtac
     9601 cccgcgtacc tgtacagcga tcttgccgca ttgtacgaac gtgcgggtca ggtcgaaggg
     9661 cggacgggtt ccatcactca gcttcctttg ctgacgatgc ctaacgagga tattacgcat
     9721 ccgatccccg acctcacggg gtacatcacg gaaggtcaga tagttctctc caaggcattg
     9781 cagggggcag ggatctatcc cccgatcaat gtgcttccgt cgctttcccg cctcatgaac
     9841 gacgggattg gcgaaggtcg cacccgggaa gaccaccgga acgtatcgag tcagctctat
     9901 gcggcgtatg cgcgagtaaa acgcgtcgaa gttcttgctg ccgtcatcgg tgaggaagag
     9961 ctgtcggaga tcgacaagca gtacctcact ttcgggcagc attttgaaaa ggagttcatt
    10021 caacaggcac cggatgagga tcgctccatc gaagagacac tgaacctcgg gtggaagtta
    10081 ctcaagtacc tccccgtttc ggaacttacc cgcgtgaagg aagaacagat cgcgaagtat
    10141 ctgccgaagg attgaagacg gtccttttga ctggaaatca aacacaccgc actggagacg
    10201 tgatcagccg cgtgaggagc tgttgaaatg ccgcgtttga acattccggc gacaaagatc
    10261 aacctcatcc gaatcaagga tgagttgaaa ctggccgtgg acggccacga gctgctggag
    10321 cagaagcggc aggtgctgat catggaagta aggaagctca taaatagtct gagagcagaa
    10381 cgcagcgaaa tggaaaacct cttgcgcgaa gcgtacgcga aactcgcaca ggcaacgtgg
    10441 gagaacggcg cggagcaagt cgcgttggca ggtctggcga cctctcaggc tattgatatc
    10501 agcctgcgag agcgcagcgt gatgggaata attctcccgg tggtagacgc aacggaggcg
    10561 gagcttcggc cctcgtacag cctttcggga acaacgagct cacttgatga ggctgcccgt
    10621 gcgtttcacg gagcgctgca acatatcgca cgacttgcgg aactggaaaa tgcggctctg
    10681 cggctggcca ctgaactgat gaaaacccag cgacgggtta atgcactcaa gaacttcttc
    10741 atcccccaat accatgaaac cgtgaaattc cttcaagaaa cgcttgaaga acgcgaacgc
    10801 gactcccttt tcgcgttgaa gcgcctcaag tcgaaagctg acctccctgc ctgaccggcg
    10861 caagggaact tggcgttcgg cccgcgattg ccatacattc aggcttgggt tgtagtacga
    10921 agctgcaacc tcacgtctga gtattcggca ccacaatcaa tcagaacagg tcgcacacat
    10981 tcatggtcag cccactcccg gttctcgaag acaaaacgct tttcgcgcgc ggcgatacat
    11041 cgatcacccc ggcacctgaa gagatctccc gcctcgtcat ttacctcgtc aaaccatcgc
    11101 gctacgacga cgacggatat cttgttcgcc attggcgcgg ggttcttccc agcaatacgc
    11161 tttcctgcct tgcggggttg actgacgatg tggtccaacg aaaagtgctg ggtgaacacc
    11221 tgaagattag cacggttctg gtcgatgagt cagtggaacg gatgccgcgg cggaaaatcc
    11281 tggcggacgc gggtcgaaag ggaacgcgtg cggttgtctg cctcgccggc gttcagacga
    11341 accagttcgc acgcgcgacc gacatcgcgc tggaatatgc atcgctgggt gtgcgcgtga
    11401 tgatcggtgg gtttcacgtc agcggcatga cggccatgtt tccggagggc acgccggaca
    11461 tcgcgcggtt gacggatgcg ggagtgacgg tcgttgccgg cgaggtggaa gatcgatggg
    11521 cggcccttct gtcagatgtg gtcaacgatc gcctgc
//
LOCUS       DAOSOO010000092        11521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5589_length_11521_cov_5.785191, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000092 DAOSOO010000000
VERSION     DAOSOO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_11521_cov_5.785191"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PLT86_07970"
     CDS             <1..1528
                     /locus_tag="PLT86_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36586.1"
                     /translation="LGCFGPDSGEFEDCGSPVAGTGNLYLRTVTTTPGGDIACTFGYE
                     RSSTQVYRVAKRRFEYLLPGNPDFTGSPLVSLRGHLFVSDGERGLMAFQGPELKPVDR
                     LPLPKCPGGKAWTGVARFSTDERVYLTVGQQLWLWLPETGRLALVHDVDLRGGNIFDV
                     AADGRLLGIRGQDYFVAQPGTRTIALRRIPAESKGRPFHFMAADLPGSPEPHRIWGGP
                     PFGQTVACYEIGSGKVINTGAVVDSGGEVYGGVALNGKIYTASYAGADFAVYDPAQPW
                     NQWEGINPRHLGSIKTHRVCRPTGRMYLAPDGNLYSGWQANYGEYGGALARLNPATEE
                     ITVWRNPLGDEPIISLAVDDTYAYMGTDHSANGLPRREGKGQFGVWSLKDERVVFQQR
                     METMEGVGHIGVISNPRIAMFPEGDALHVFNAEKMEFERDLAAAVPDSPGWCEDVLRS
                     FDGTWVLYARGNHLIKVHQDLSVEVLPPIEHAINHMVFGPDGNLYFSSGTTLYVLEGL
                     "
     gene            1623..3593
                     /locus_tag="PLT86_07975"
     CDS             1623..3593
                     /locus_tag="PLT86_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36587.1"
                     /translation="MAQSLSSPSESNESAAPGRANLVVTALADPVRAKQVLAGRAAVD
                     PVDGREKFWITNMNEQYGGELIAIDFEGDTAEVFRWPAGQGSWCVLPLPRERLAISTY
                     YDGKFLVFDMRRREFTRVVSFPGESYVWEMATGSDGRVYGGTYPGAKLGCFDPETGEF
                     EDCGSPVAGTENQYLRNVATAPGGNIICTFGSASQSIRVYRIAEKRFEFLVPDEPDFF
                     AKPIVSLRGHLFVQHPERGLLAFRGPRLEPVEQLPLPECPVGSGWKGIARYSTEERVH
                     LTAERQTWEWLPATASLTLVTDLTLRGCRVFDVAQDGRLLGIRGQDYFALPAGSPRLE
                     LRRIPAESAGRPFHFLTADPPDSPRPHRIWGGPSYGQTVASYEIETGKVWNSGAVIDS
                     SGEVYGAVSVGGKLYTASYSGADFAVYDPARPWDQWNNVNPRHIGSISDQHQCRPTGR
                     MRLGPDGNLYSGWQAAYGEYGGALARLNPRTEEITVWRNPLGDEPVISLAVGGRFAYL
                     GTDQTANGLPRREGKGQFGVWSLAEERVVFQQRMETMNAVRCIGAMRTKELAFFPERD
                     ELHVFDGESLQFVEKIPAPVPDSPGWCEDVLLHPSGYLLYARGPFLVRVAPDLRVEIL
                     GPLEKPVNHMTFGPDGLLYFSSSTTLYSLRGL"
     gene            complement(3576..4373)
                     /locus_tag="PLT86_07980"
     CDS             complement(3576..4373)
                     /locus_tag="PLT86_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36588.1"
                     /translation="MPHVVLVIPDRALRLLWEHAFSVAGWRTDPADTAIDALAAAFNA
                     EADAIVVALTDAISAEIGRETLAELEKDSELSAIPRFALAVDGPGADVPGVRVFPQPC
                     DPFWLICEADAALGRETPRGLGRRPFSESDTDVMVHAWWWTLAAGDLVQRVMRFDADG
                     ITGEALAVKVAELLGLRADVQIEIGHGDPPQGARNFRYYGEIGGVENGIVILNDSAAS
                     ETWWRVTLPREGVSRGVAEAIAAVARALRIILRRDAAAGGCISQTPE"
     gene            complement(4383..5900)
                     /locus_tag="PLT86_07985"
     CDS             complement(4383..5900)
                     /locus_tag="PLT86_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOT36589.1"
                     /translation="MVEFPVAECWVIEPTDPPHGFHDLVGYLINAGVDISFGPNDTLP
                     NDLPPSCDGIRVIVTCLEDLPRLRRRLRGFVGLDPDTDSLEGVAPHGTMYLHENGRTW
                     LAAYSEKGRFETKRIENPLIVAPDLTINSPAMRARMLARSDRDVHRTLLEGLMQSDRH
                     EWSDVSFGTLWAMIGAFEATGEERYREAAVRFADLQLARWDADGRKDGGPEISALAFL
                     RLSEVSGDPRYRKEILAAIPEVPEKSRYRPAFGAVDDSRKQVGTGLLLQAHSFVHPAT
                     RAVLESRAADGGTYSEQVGLELASVLAAARAGGWSHDATPVIAACLAAHRENCRDPET
                     GLYYHGLLGRRGAHKGFQGHGMLWTCFGLQNTLEQWPTDDPAYAEVLRMYGDACDAAA
                     TVQDPETGEFHHILDMPQSPFGRIYTPALAYVFLRGARLGLLSGEFRERGIRAWEAMK
                     CHIFQGGSVGGDAGTPQMKRFEAYLLQPVTYSYRSNRTRCFWQAHAANEVLRTIA"
     gene            complement(6053..6550)
                     /locus_tag="PLT86_07990"
     CDS             complement(6053..6550)
                     /locus_tag="PLT86_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT36590.1"
                     /translation="MDALTVIKTRRSVRAFADRQVPREVLEDIVDCGRLAATARNEQP
                     WEFIVVTDPAMRAKIADLAEYGKFIAQASACIVVVCKDGRYFLEDGSAASQNILLGAW
                     AHGVGSCWVAGDKKPYAVDVAKVLGVPDGYRVISLIALGYPQAPPLPKPKRALKEVLH
                     WEKWA"
     gene            complement(6516..7298)
                     /locus_tag="PLT86_07995"
     CDS             complement(6516..7298)
                     /locus_tag="PLT86_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOT36591.1"
                     /translation="MYKLIACEVARPEFDRVISMSRKSVSVEYLEQGHHNDVVRGRKA
                     IQERIDAAGEGEWEALLLGYALCNNMLVGLQARVKPLVIARAHDCITWFLGSKERYAS
                     TFEEAPGTYYYTAGWLKVVRSHDQAGQSASASGVLVEGEYARLAEQYGEDNAAYLWEM
                     SNAWIKNYRYGAYIAVPGIDHDGHRKRVQEICARNSWEYKEIEGDLGLLQRWVDGEWD
                     AKDFLIVKPGDTIAPTPDAQIISVGRKEGGQNGRIDRHQDAP"
     gene            complement(7313..8476)
                     /locus_tag="PLT86_08000"
     CDS             complement(7313..8476)
                     /locus_tag="PLT86_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36592.1"
                     /translation="MALMIDFDTHNAEVEAVWKAYRERKPTRVPMVFGINPRLTMFDH
                     PSNPRGITFEQYSSDPQLMMERQIEHQAWVRTNILQDAPMGLPKDGWSLWVDFQNYYE
                     AAWYGARIRYWPGEVPDTEPLLEDDASKWSLIEKGMPDPFTHGSAQRMWDYYEYFCRK
                     KEEGWTYLGLPIATVTWPAGGTDGPLTLCCNLRGASRFIPDMIEDPEYAHAMLNYITE
                     TSVARIKAYRERMGAELKPERGGLADDSVMLISTPMYREMILPYHRRIFEELAGKGPH
                     SIHLCGDATRHFKTIRDELNVWSFDTGYPVDFGWLRRELGPEVEIYGGPSVPFLRAAT
                     PDAVGAEVTRILGSGIMEGGRFVLREGNNLAPGVPMENLRAMWDAVHEFGTYA"
     gene            9200..>11521
                     /locus_tag="PLT86_08005"
     CDS             9200..>11521
                     /locus_tag="PLT86_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36593.1"
                     /translation="MSALTHLCMHFHCDPIFTCPQATHIEESVELAAQYVSMCREDPE
                     FRCILSETDYLHGYWNLYAEDREFLLRLMEEGRVETSGSYSEPNENSVGGEALVRNIL
                     YGQWFQVGYLGGRGDIYMPHDVFGHVRQLPQILRKAGFIATTWTKGSPKPGAPDPCFQ
                     WLAPDGSSILTRVTSYGGVDWGASDQIGGVLKAGDAYPGDAPIPNLLFRGRDFVSPPG
                     WISGKGSALRDHRVLMSQPSAFFEEVSARIKAGEFHPPLTAREHSQYHVGTALSRIEL
                     KIAMRIAEETLGDAETWSGIATLYGHPYPEAEIDHAWRQLLFSQHHDSITGTSNDQSY
                     LDLLTHLREALGEGISARRTATSHLAEEITTDGRTGIPVVVFNSLGHTRTDVVEVPLP
                     HAFTGTRVRALNDTGVQTPVAVVQRETGKSARFLAEHVPGLGYSTYWLSDGDAEDSPR
                     EEVIESDDAPLVLETPFARITVDPLRGGGIVSLIERKSGREIASGASPYPLNDFVLLK
                     EGPGVEPSWEFCTTGDRVFASSIRAHVRAERTALGWRVVIRQPFPECDHLVRTLELFD
                     HTPRIECSVILSGYAGSRLVGSDTNTRPWGGSNEGKNPEPVDRDMFGVLFPVGLRGLA
                     PVWSDRFGQRVIRRSRSFLDFRTHQGRIYSGCAMFSAEKWIATSPSVSLVGESGDGSR
                     VGIPLGMVRVIYPDDPVAREAADLIVVALAEQGVTATPYLDTENQNDDVLQTDQIVAI
                     GGPESNAWVATHREWQPIGNRFLTSVSLAEDASR"
BASE COUNT         2220 a         3621 c         3395 g         2285 t
ORIGIN      
        1 actggggtgt tttggccccg attcagggga atttgaagac tgcggatcgc ctgttgccgg
       61 aaccggcaat ctttacctgc ggactgtcac gacaacgccg gggggagaca ttgcgtgcac
      121 cttcggctac gaaagaagtt cgacccaggt gtaccgcgtg gcgaaaagac ggttcgaata
      181 cctgcttccc gggaatccgg acttcacggg aagcccgctt gtgagcctcc gcggccatct
      241 tttcgtgagc gacggagaac ggggactgat ggcgtttcaa ggtcccgaac tgaagccggt
      301 ggatcgcctg ccgctcccaa aatgccccgg cggaaaagcg tggaccggcg tcgcgcggtt
      361 ttcgacggac gaacgggtgt acctcacggt cgggcagcag ctctggctgt ggctccctga
      421 gacaggacgg ctcgcactcg tacatgacgt cgatctgcgg ggaggaaaca tcttcgacgt
      481 ggcagccgac gggcgcttgc tcggaatcag agggcaggat tacttcgtcg cccagccggg
      541 aacccgcacg atcgcattgc ggaggattcc cgcagagagc aaaggccggc cgtttcattt
      601 catggcggcc gacctgcccg gttcgccgga gccgcatcgg atatggggag gccccccctt
      661 cggccagact gtggcctgct acgaaatcgg gtccgggaag gtgatcaata cgggggctgt
      721 cgtggattcc ggaggtgagg tgtacggcgg ggttgctctc aacggcaaaa tctacacggc
      781 gtcgtacgcc ggggcagatt tcgcagtgta cgatccggca cagccctgga atcagtggga
      841 aggaatcaac ccgcgtcatc tggggtcgat caagacccat cgcgtgtgtc gtcccaccgg
      901 ccgaatgtat ctggcgccgg atggcaacct gtattccgga tggcaggcca attatggcga
      961 gtacgggggt gcgctcgccc gattgaatcc ggcaacggag gaaatcaccg tgtggcgaaa
     1021 cccgctggga gacgagccga tcatctcgct cgcggtggac gatacgtacg cctacatggg
     1081 aaccgaccac tctgcgaatg gtctcccgcg acgggaagga aaaggtcagt tcggggtgtg
     1141 gagtctcaag gatgagagag tcgtgttcca gcagcgcatg gagacgatgg aaggagtcgg
     1201 tcacataggg gtgatatcca atccgcgaat cgcgatgttc cccgagggag acgcattaca
     1261 cgtattcaat gcggaaaaaa tggaattcga acgcgacctc gcggcggcgg ttcccgattc
     1321 ccccggctgg tgtgaggacg ttctgaggtc gttcgatggc acgtgggtgc tctatgcacg
     1381 aggcaaccac ctcatcaagg ttcatcaaga tctttccgtg gaggttctgc cacccattga
     1441 acacgcaata aatcacatgg ttttcggccc ggacggaaat ctgtacttct ccagcggaac
     1501 aacgctgtac gtcctggaag ggctgtgagg tgataatgaa ccgccgggag tttcttcgat
     1561 ggtcgggagc agggatcggt tctctcctgc ttcccgcacg tctgcttgca aggaagcaca
     1621 gcatggcgca gagcctttcc tcaccgtccg agagcaatga atcggcagcg cccggccgcg
     1681 ccaacctggt cgtgacggct ctcgcagacc ccgtccgggc aaagcaggtg ctcgcgggcc
     1741 gcgcagcggt tgaccccgtg gatggtcgcg agaaattctg gatcaccaac atgaacgagc
     1801 agtatggcgg tgaactgatt gcgatcgact tcgaagggga caccgccgaa gtcttccggt
     1861 ggcctgccgg gcaaggatcg tggtgtgttc tcccgttgcc acgcgaacgg cttgcgataa
     1921 gcacgtacta tgacggtaaa ttcctcgtgt tcgacatgcg aaggcgggag ttcacgcgcg
     1981 ttgtgtcatt ccccggtgaa agctacgttt gggaaatggc cacaggatca gacggccgcg
     2041 tgtacggcgg aacctacccg ggcgcaaaac tgggatgttt tgacccggag acgggagagt
     2101 tcgaagactg cgggagcccg gttgccggaa cggagaacca gtatctgcgc aacgtcgcga
     2161 cggcgccggg aggaaatatt atctgcacgt tcggatcggc aagtcaatcc attcgcgttt
     2221 atcgaatcgc ggaaaagcgg tttgagttcc tcgttccgga cgagccggat ttcttcgcca
     2281 aaccgatcgt cagcctccgt ggccaccttt tcgtgcaaca ccccgagcgc ggccttctgg
     2341 cattcagggg tccacgattg gaacccgtgg aacagctccc gctccccgaa tgccccgtgg
     2401 gaagcgggtg gaaagggatt gcacggtatt ctacggaaga acgtgttcac cttaccgccg
     2461 aacggcagac gtgggaatgg cttccggcaa ccgcttctct gactctcgtg acggacctga
     2521 cactgcgcgg ttgccgggtt ttcgacgtag cacaggatgg tcgcctgctg ggcatccgcg
     2581 gtcaggatta cttcgcattg cccgccggaa gcccgcgttt ggagcttcgc cgaattccgg
     2641 ccgaaagcgc cgggagaccg tttcatttcc tcactgcgga cccacccgat tcgccccgtc
     2701 cccaccgcat ctggggcggc ccatcgtacg gacaaaccgt cgcctcgtat gagatcgaga
     2761 ccgggaaagt ctggaattcc ggggccgtga ttgactcaag cggagaagtg tacggcgcgg
     2821 tttctgtcgg tggaaaactg tacactgctt cgtattccgg cgcggacttc gccgtgtacg
     2881 atcccgcacg cccgtgggat caatggaaca acgtgaaccc gcggcatatc ggctccatct
     2941 cggaccagca tcagtgccgg ccgacaggac gaatgcggct cggccccgac gggaatcttt
     3001 actccggatg gcaggcggca tacggggaat atggcggggc tctggctcgt ttgaacccgc
     3061 gaaccgagga aatcaccgtc tggcggaatc ccctcggcga cgaacccgtc atctcgcttg
     3121 ccgtcggcgg ccgctttgcg tacctcggaa ccgatcagac cgcaaacggt ttgccgcggc
     3181 gagaagggaa agggcagttc ggcgtgtgga gccttgccga ggagcgcgtg gttttccagc
     3241 agagaatgga aacgatgaat gcggtccggt gcatcggcgc gatgcggaca aaggaactgg
     3301 cgttcttccc cgagagggac gaactccacg tgtttgacgg ggaatctctt cagttcgtgg
     3361 aaaaaatccc cgcgccggtg cctgactcgc cgggatggtg cgaggacgtg ttgctccatc
     3421 cctcagggta cctgctgtac gcgcgaggac cttttctcgt gcgggttgcc cctgatcttc
     3481 gcgtagaaat cctcggtccg ctggaaaaac cggtcaatca catgaccttc gggcctgacg
     3541 ggctcctgta tttttccagc agcaccacgt tgtactcact caggggtctg tgagatgcag
     3601 cccccggcgg ctgcatcccg ccggagaatg attcgcagcg cgcgcgcgac cgcggcaatc
     3661 gcttccgcca ctcccctgct cactccctcc cggggaagcg tcacccgcca ccaggtctcc
     3721 gaggcagcgg aatcgttcaa tattacgatc ccgttttcca ccccgcctat ttccccataa
     3781 taccggaagt ttcgcgcccc ctgcggcgga tcgccgtgcc cgatttcgat ctgaacgtcg
     3841 gcgcgaaggc ccaaaagctc cgcgaccttc accgcaagcg cctcacccgt tatgccgtcc
     3901 gcatcgaatc tcatcacccg ttgtacgagg tcacccgcgg cgagtgtcca ccaccaggcg
     3961 tgaaccatga cgtcggtatc gctttcgctg aacggacgcc tgccaagacc ccgtggcgtc
     4021 tcccgtccca atgccgcgtc agcctcgcag atgagccaga acgggtcaca gggttgtgga
     4081 aacacgcgca caccgggaac gtccgccccc gggccgtcga ccgccagagc aaatcgcgga
     4141 atggcgctca gctccgaatc cttctcgagt tccgccagcg tctcgcgccc gatttccgcc
     4201 gatattgcat ccgtcagtgc caccacgatc gcatcggcct cggcgttgaa cgctgcggcg
     4261 agtgcatcta tcgcggtatc ggccggatcg gtacgccacc ctgcaacgga aaacgcgtgt
     4321 tcccacagca agcggagtgc gcgatccgga atcaccagca ccacatgggg catggagcac
     4381 tcctacgcga tagttctcag gacctcgttg gccgcgtgcg cttgccagaa acaccgggtg
     4441 cggttgctcc ggtagctgta cgtcaccggc tgcaataggt aggcctcaaa ccgtttcatc
     4501 tgcggtgtcc cggcatcacc gccaacgcta cccccctgaa agatgtgaca tttcatggct
     4561 tcccacgcgc gaattccgcg ctcgcggaac tcgccagaga gcaatcccag ccgcgccccg
     4621 cgcagaaaca cgtatgccag cgcaggggtg tagattcggc cgaaaggcga ctgcggcatg
     4681 tcgaggatat ggtgaaactc accggtttcc ggatcctgaa cggtggcagc cgcgtcgcat
     4741 gcatccccgt acatacggag aacttccgcg tacgccgggt catcagtcgg ccactgctcc
     4801 agtgtgttct gcagcccgaa acaggtccac agcatcccgt gtccctggaa gcctttgtgt
     4861 gcgcccctcc ggccaagcag gccgtggtaa taaagcccgg tttccgggtc gcgacagttt
     4921 tcgcggtgag ccgccagaca cgccgcaata accggagtcg cgtcgtggct ccatcccccc
     4981 gcccttgcgg cggccagaac ggaagccaac tccagcccta cctgttcgct gtacgttccg
     5041 ccgtccgccg cgcgggattc gagaaccgca cgcgtcgcgg ggtgaacgaa actgtgcgcc
     5101 tgaagcagca ggccggtgcc gacctgtttc cgggaatcgt ccactgcgcc gaaggccggg
     5161 cgataacgcg acttctccgg tacttcaggg atagccgcga ggatttcctt gcgataccgt
     5221 gggtcgcccg atacctccga caatcgcagg aacgcgaggg ctgaaatctc gggcccgccg
     5281 tctttcctcc cgtcggcatc ccaccgcgcg agctgaagat ccgcgaaacg gacggcggct
     5341 tctcggtacc gttcctcccc cgtcgcttca aacgccccga tcatagccca gagcgttccg
     5401 aaactcacgt ccgaccattc gtgccggtcg gattgcatga ggccttcgag cagcgttcgg
     5461 tgaacgtcgc ggtcggagcg ggccagcatc ctcgcacgca tcgccgggga gtttatcgtg
     5521 aggtcggggg caacgatgag gggattctct atccgtttgg tttcgaaccg ccctttttcg
     5581 gagtaggctg ccagccacgt gcgaccgttt tcgtgcagat acatggttcc gtgcggggcg
     5641 acgccctcca gcgaatctgt gtccgggtcg agtccgacga atcctcgcag gcgtcgtcgc
     5701 aggcgtggca gatcctccag gcacgtgaca attacgcgga ttccgtcgca ggagggcggg
     5761 agatcgttcg gcaacgtgtc gttcgggccg aaggaaatat ccactccggc gttgatcaga
     5821 taaccgacga ggtcatgaaa cccgtgcgga ggatcggtag gttcgatgac ccagcattct
     5881 gcgaccggga actcaaccac ggagcccctc ctcacccgcg ttcgacaatg ttcggatacg
     5941 caacgtcgtc tgtcctttct ggaaccgagc ccctccgaag ttcgccggat gagccctggc
     6001 cacgcaggaa ggttgcttcc cgctcttttc cgggatcaca gattcggcgt gttcacgccc
     6061 atttttccca gtgcaaaacc tctttcagcg cgcgttttgg tttgggcaag ggcggcgcct
     6121 gagggtaccc aagcgcaata agcgaaatca ctcggtaccc atctggaaca cccagcacct
     6181 ttgcgacatc gacagcgtac ggtttcttgt cccccgccac ccagcaggaa ccgactccgt
     6241 gcgcccacgc gccaagcagg atgttctgag acgccgccga gccgtcctcg aggaaatacc
     6301 ggccgtcttt gcacacgacg acgatacacg ccgaggcttg tgcgatgaat ttcccgtact
     6361 ccgcaaggtc cgcgatcttc gcgcgcatgg cgggatctgt cacgacgatg aactcccagg
     6421 gttgctcgtt gcgcgccgtt gccgccagtc tgccgcaatc cacaatatct tccagtacct
     6481 cgcggggaac ctgcctgtcg gcgaacgcgc gcacgctacg gcgcgtcttg atgacggtca
     6541 atgcgtccat tctgacctcc ttcttttctg cccactgaaa tgatctgtgc atccggggtc
     6601 ggagcgatag tgtcacccgg tttgacgatc aggaaatcct tggcgtccca ctcgccgtcc
     6661 acccatcgct gcagaagccc gagatcccct tctatctcct tgtattccca ggaattgcgc
     6721 gcgcatatct cttgcactcg tttccggtgc ccgtcatggt ctatgcccgg gacggcgata
     6781 taggctccgt accggtagtt cttgatccat gcgtttgaca tttcccagag atacgcggcg
     6841 ttgtcttccc catattgttc cgcgagcctg gcgtactcac cttccaccag cacgcccgac
     6901 gccgaggcgg actggcccgc ctgatcgtga ctgcgtacca ctttcaacca gcccgccgtg
     6961 tagtaatatg tccccggagc ttcttcaaat gtgctcgcat accgctcttt cgacccgagg
     7021 aaccaggtaa tgcagtcgtg ggcgcgcgcg ataacaagcg gcttgaccct cgcctgcaac
     7081 cccaccagca tgttgttgca cagagcgtac cctagaagca aggcttccca ttccccttcg
     7141 cctgcggcgt ctatacgctc ctgaatggct ttcctgcctc gcactacgtc attgtgatgc
     7201 ccctgctcaa ggtattccac ggaaaccgac ttccgcgaca tcgagatgac gcggtcaaac
     7261 tccggtctgg caacctcgca ggcgataagc ttatacacac cccaatcctt cctcacgcgt
     7321 acgttccgaa ctcgtgcacc gcgtcccaca tcgcccggag gttctccatg ggtacccccg
     7381 gtgcaaggtt gttcccctcg cggagaacga accttccgcc ttccatgatc ccgctgccga
     7441 ggattcgtgt gacctcggcg ccgactgcgt ccggtgtcgc ggctcgcagg aacgggacgc
     7501 tgggaccgcc gtagatttcg acctccggcc cgagctccct tctcaaccag ccgaaatcca
     7561 cgggatagcc ggtgtcgaag ctccacacgt tgagttcgtc tcgaatggtt ttgaaatgcc
     7621 gcgtcgcatc gccacaaaga tggatactgt gcggtccttt tccagccagt tcctcgaata
     7681 tgcggcgatg atagggcagg atcatctccc ggtacatcgg cgtgctgatg agcatgaccg
     7741 aatcgtccgc gagacctcca cgttcaggct tcagttcggc gcccatgcgt tcgcggtacg
     7801 ctttgattcg agcaacgctc gtctctgtga tgtagttgag catggcgtgg gcgtactcgg
     7861 gatcctctat catgtcggga atgaacctgc tggccccccg caggttgcag cagagggtca
     7921 acggaccgtc ggtgccaccg gcaggccatg tcaccgtcgc gatgggcaac ccgaggtaag
     7981 tccacccctc ctcctttttg cggcagaagt actcgtaata atcccacatg cgctgcgcgc
     8041 tgccatgagt gaaggggtcg ggcataccct tttcgatcag cgaccatttc gacgcatcat
     8101 cttccagcaa gggctccgtg tcgggcactt cacccggcca gtaccgaatg cgcgccccgt
     8161 accacgcggc ttcatagtag ttctggaaat ccacccagag gctccagccg tccttcggca
     8221 gacccatcgg ggcgtcctgg aggatattcg tccgcaccca cgcctggtgc tcgatctggc
     8281 gctccatcat cagttgggga tcgctggagt attgctcaaa ggtgatccca cgggggttgg
     8341 acgggtggtc gaacatcgtc aggcgcgggt tgatgccgaa aaccatcggc acgcgcgtcg
     8401 gtttgcgctc gcggtatgcc ttccagactg cttccacttc cgcgttgtgc gtatcgaaat
     8461 cgatcatcaa agccatctgg aggtctcctg cgaattcgtg tgctgtacgt cacatctttg
     8521 gcgttgcgcg aaaggtatct tttgcttgga agactgaaca tcaaccctgg gacattgaat
     8581 atcagtcaat ggacacacca acggacaccc catgagcgca cccagaaact ggtacccggc
     8641 acgcgagagg attcttgact tcatcggcac aacgctcacg ttcctcggcg tattggtact
     8701 cgttctctat ctgaccggat ttttcggata gacaccccgc aaggtcagct tttcactagt
     8761 atacccctgc cgattccacg taccaacgcc gcagatcgtt gcgcccgcgc agcacggtcg
     8821 tcgagtggcg aacgcggcgg cgttcgttgt atcgagacgc ccccgcatct gccgagagac
     8881 gtgcaatgcc gcacagcgga gtgcgcggac taccgtttga gtgcgagtgg cggttgcgct
     8941 atgcagtcag gttgccccac cggacagcgc gccccaacac gcagggggcg tccagacacg
     9001 cagtagatgg tgtggcggac attcctgtcc gccacaacct gcaaaagctc gccacttctc
     9061 cctcggcctg cccgcatcac gcttggttcc ccggggtctc ggcgttttct ttagtacttg
     9121 aataatccat ctcagccatc ctcacacctg acaatcgcca cttcgctacc ccccgatcac
     9181 gccatcagaa aggcaataga tgtccgcgct tacccacctc tgcatgcatt tccactgcga
     9241 cccgatcttt acctgcccgc aggccacgca catcgaggaa tccgtcgagc ttgccgcgca
     9301 atacgtgagc atgtgccggg aagacccgga attccgttgc attctttccg aaaccgacta
     9361 tctgcacggc tactggaacc tttacgcgga agaccgggag ttcctgctcc gtttgatgga
     9421 agaaggacgt gtggagacgt cgggttcgta cagcgaacca aacgaaaaca gcgtcggcgg
     9481 cgaagccctc gtaagaaaca ttctttacgg ccagtggttt caggtcggct acctcggcgg
     9541 ccgcggcgat atctacatgc cgcacgacgt gttcgggcat gtgcgccagt tgccacagat
     9601 cttgcgcaag gccgggttca tcgccaccac atggacgaaa gggagcccca aaccgggcgc
     9661 acctgatccg tgcttccaat ggctggcgcc cgacggctcc tccatcctca cccgcgtcac
     9721 gtcgtacggc ggggtggact ggggcgcttc cgaccagatc ggtggtgtcc tgaaggccgg
     9781 agacgcctat ccgggggacg cgccgatacc gaatctgctt ttccgcgggc gcgatttcgt
     9841 ctccccgccc ggctggatca gcgggaaagg cagcgctctt cgggatcatc gtgtccttat
     9901 gagtcagcca tcagcgtttt ttgaagaagt ctccgcccgc atcaaggccg gggagttcca
     9961 tccccctttg accgcacgag agcacagcca gtaccacgtg ggaactgcac tcagccgtat
    10021 tgagctcaaa atcgcaatgc gcattgccga ggagaccctc ggagacgcgg agacctggtc
    10081 cgggatcgcc acgttgtacg gacatcctta ccccgaagca gaaatcgacc acgcgtggcg
    10141 gcagcttttg ttttcccagc atcacgactc catcacggga acatcgaacg accagtcgta
    10201 tctcgacctg ctcacgcacc ttcgtgaagc gctgggagag ggaatttctg cgcgcagaac
    10261 ggccacgtcg catctcgcgg aagagatcac cactgatggt cgcaccggca ttccagttgt
    10321 tgtgttcaac agcctcggtc acaccagaac cgacgtcgtg gaagtgccgc ttccccatgc
    10381 gttcaccggc acgcgtgtac gagcgttgaa cgacaccggg gttcagacgc cggtcgcagt
    10441 cgttcagcgg gaaacaggaa agagcgcccg gttccttgcg gaacacgttc caggactggg
    10501 ttattcgaca tactggcttt ccgacgggga tgcagaagac tccccccggg aggaagtcat
    10561 cgagtccgat gacgcgcctt tggttctgga aacccccttc gcccgcatca cggtggaccc
    10621 gctccgcggg ggtggcatcg tcagcctcat cgagcgcaag agcggtcggg aaatcgcctc
    10681 cggagcatcg ccgtatcccc tcaacgattt cgtgcttctc aaagagggcc caggggttga
    10741 accctcatgg gagttttgca ccacaggaga ccgggtgttc gcatcctcca tcagggcaca
    10801 cgtgcgcgcg gaaagaaccg cgctcggatg gcgcgtggta atacgtcagc ccttccccga
    10861 atgcgatcat cttgttcgga ctctggagct ttttgaccac accccacgga ttgagtgttc
    10921 tgtcatcctc agcggttacg cgggaagccg gctggtgggc tccgacacga acacccgccc
    10981 gtggggagga tccaacgaag gcaaaaaccc ggagccggtc gaccgggaca tgttcggagt
    11041 tctgtttcct gtggggctca gagggttggc tccagtctgg tcagaccgat tcggccagcg
    11101 ggttatccgc cgatcgcgtt catttctgga tttcagaacg catcaggggc gcatttactc
    11161 cggatgcgcc atgttcagcg cggagaaatg gattgccacc tccccgagcg tctccctcgt
    11221 tggagagtcc ggggatggtt cccgcgtcgg aattccgctt gggatggtcc gggtaatata
    11281 tccggacgat cccgttgcgc gtgaagcagc cgacctcatc gtcgtcgccc ttgccgaaca
    11341 gggagtgacc gcgacgccgt acctcgacac ggaaaaccag aacgacgacg tcctccagac
    11401 cgatcagatc gtggcgatag gtggtcccga gagcaacgcc tgggtcgcca cccaccgcga
    11461 gtggcagccg attgggaacc ggtttcttac cagcgtgagt ctcgcggaag acgcttctcg
    11521 g
//
LOCUS       DAOSOO010000093        11333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5720_length_11333_cov_6.572087, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000093 DAOSOO010000000
VERSION     DAOSOO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5720_length_11333_cov_6.572087"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLT86_08010"
     CDS             complement(<1..595)
                     /locus_tag="PLT86_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT36594.1"
                     /translation="MTIHERIEWRDIWVMGADDDTTPRVLLVGDSITRSYYDHVRDAL
                     NGRFLCARLTTSTSVCDAAMEKELALLLDDYRFAVIHFNNGLHGFGYGEAEYAAGLAR
                     VLDFILKQSPQSRLIWASSTLFLRTDGSGAPDPRTARVRERNRLAAELAAARGVPVND
                     LFSRVIDRPELFSGDGVHLNEAGQRILGAQVVEAILAA"
     gene            complement(1160..2050)
                     /locus_tag="PLT86_08015"
     CDS             complement(1160..2050)
                     /locus_tag="PLT86_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT36595.1"
                     /translation="MLRKHSETIVNKVYPVPETPTYLPGSVIEIVNPDIDRGHIKHAV
                     FDFDGTVSVLREGWPDIMLPVMVDAIRGDHPATPEIVERCKKFIDETTGIQTFFQMEG
                     LVHMVEEFGLVPKDKILDAWGYKAIYNEALMKPVRERIAALERGEKTLEDVTMFGSRE
                     FLKALYDRGVRIYLASGTDQEDVRNEAHACQIDQWATGGIWGAIRNVDDYSKDKVMKD
                     IISQNNLHGAELMVVGDGPVEIRNAKDNGAIAVGVASDEVARRGLNAHKRQRLIKAGA
                     DIVIPDFLEWKKLLKYLFNE"
     assembly_gap    2218..2317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2318..4269)
                     /locus_tag="PLT86_08020"
     CDS             complement(<2318..4269)
                     /locus_tag="PLT86_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36596.1"
                     /translation="MKRAEPEMAVLVRLDLSGIPFGPVLRPMEPVPEAERGLFSTWET
                     PLSPWGGFDVGAGLACVDDAGRPALECAEGERHERAIVARKVDLRDGTIVAEVKPLAA
                     NALPNDDSPVQTEALAGIVFRVQTSRAYYLFGIQGKRRVVLLRRNDDEFAVLAEQAIA
                     APQDYVTLEVRLDGDSIRCTSPELGVDMHVCDTVYHAGKVGFRSANSSRLRRFEVSQT
                     PGQRRRDESRHARFEREVAERGAGIPDATLVRTYGTADLGGSPVFRDFSEPGRFDILV
                     EGKQLRALTPEGGLIWEVPERIVRCVFSRDFGPAGRLIYGFAGKREERRAKDIAGGES
                     VLTVENEMVVIEGRTGKIVARAELPPNVPTQRFFDWSPRSASLANSDGTDIVLREWRD
                     DHGGGGIRLWAYDRGLNLLWEHVQTGAHYGHHWALDFFDVDGDDREEMLAGGYLYRGD
                     GTILWTHDRADEMQQIRGAGHYDAVVMGNLTGEAEDDPIAFLCAGSAGVYVVDALTGA
                     TRVVHRIGHAQGRSIGKLRPDLPGTEVLAVTRWGNYGILTLFSGRGERLWTIQPDYVG
                     QGATPVKWGKAGHRLIWTNTSRDAQLFFDGYGRLVKQLPELSRVWGDRMNRDVGRGGS
                     PIRIGTDPTDLLTLTVGGVVHVFGPA"
     gene            complement(4294..5298)
                     /locus_tag="PLT86_08025"
     CDS             complement(4294..5298)
                     /locus_tag="PLT86_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT36597.1"
                     /translation="MPTRIAILGAIVYDEIITHDGQHIESFGGIVYNVAALSSFIEDG
                     VVLPVSNVGFDRYDQVLALMAGMDHVDTSQLLRTDTPCTHAKLIYHGSNYREEIVRHM
                     MPPFSAPRIISAVNGDASTPAADGILINFVNGTELDLPTLQALRKQTKAPLYLDMHNI
                     MVRFDADGKKQYLDFVEWPEWVEQFDFVQMNEFECEKVLHTSPSSPKEFLHAAQTVLA
                     AGAAAVVITMGPAGVALAHRREGIEYGCIIPAVTVKNFVDATGCGDAFSTGTFWHYLR
                     TGNPLKAAMAGTLVGALNCQVRGVGKLKRARNADQRIAEVSPQLADRVEHGWLGDRLN
                     "
     gene            complement(5652..6668)
                     /locus_tag="PLT86_08030"
     CDS             complement(5652..6668)
                     /locus_tag="PLT86_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36598.1"
                     /translation="MKQLQLCLLGGLTMLLAGVADSGNVPVTDDYASPVLDWWGGFRR
                     YSGPRQFESAIQRVEENQKFVPTGELRARKSFRNYVVKIYGHGYGIYDDGGSVEITRN
                     GTRVFAGHSGRIEIACVDGGEGDTCTTTLIGRDVTGDGIPDLVLTGYSGGMHCCHSLD
                     FFELGSQFRHIQSVYSDDAHFSRFVNLDADPALEIEVDDASYAYGRVWTGPIFNPIIL
                     KFQNGQYRMAGNLMRKPPMTRRELETLADSARAYSYLEDGLRHDMLSLIYTGNARQAW
                     ELCALTWPPDVAGRQAFLTTFVTNLPKGRYWRELLEMNGREAMREAIGWLPGEEDIPP
                     SLRE"
     gene            complement(7037..8275)
                     /locus_tag="PLT86_08035"
     CDS             complement(7037..8275)
                     /locus_tag="PLT86_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36599.1"
                     /translation="MGISLGLVGLGSFGSAFAPLFKSHQAVDRIAFCDLNPASLAKFV
                     NDPFYRDKFDPKDTFASLDDILAADLDALAIITQPQLHAPQCVAAMEAGKHVYSAVPV
                     ISLPDFDEILEWCDRIIRTCEKTGMRYMLGETTVYRPKTMFCRRMADRGEFGTFVYTE
                     GEYIHDVDAQCNLRDVAASRAANPSAVPRGTVLTTRSGTEPKGSPMSYPTHSVSGPVH
                     VMRAHAVKASAIGFRNRTGDPYFARQEFSNVVAFYRMSNGATCRICEFREAPGYFGDD
                     AETFRIAGTAGTFSNGMWWRNGRTQAGRVPPPTCQTPTDEEMRDPLPSEVVNAFKRVE
                     YRGTPESELAHLDFTPTGHGGSHPYLVHEFVTAVAENRQPVINAWEAARYMAMGAMAH
                     KSALRDGEWLEVPDWGDAPG"
     gene            complement(8337..9686)
                     /locus_tag="PLT86_08040"
     CDS             complement(8337..9686)
                     /locus_tag="PLT86_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOT36600.1"
                     /translation="MPERKAVTQLARKKRVARRDVLDGEMQEAIRRFIPSEAFGLLGK
                     SCVTDVRPGDHVEKTMTILFSDIRDFTPLSERMSPAENFDFINAYFRRMESVFVRHNG
                     IIDKYMGDAVMALFPTSPDDAVHCALEMLQRLNGFNNALKRKRGQAPIRVGIGLNTGL
                     TMLGVVGGRSHLESTVISDAVNLAARIETLTKSYGTPLLISEHTYYGARNIGHHDTRF
                     VDRVKVKGKEQPQSVYEVFDADPPLLREAKRRTKGLFEEALARYHMKEVPAATELLAQ
                     CLAEALDDTAAQVYRARCERFMETGVHESSGEFDLMIEWNPAVAVGCEEIDEQHRALF
                     DFVKGLVQAIQKTRNLSEIRTAISFLDNYVAEHFTAEEKLMAEYDYPFLPMQVAEHQR
                     FVDRFGKFKEEIRKNFRTRRNFLLFHAQILLIDWVVNHTSKLDRHFGRYLNRRDAER"
     gene            complement(9887..11173)
                     /locus_tag="PLT86_08045"
     CDS             complement(9887..11173)
                     /locus_tag="PLT86_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36601.1"
                     /translation="MRDLPLTSPVPISDRFETYQAFSPAVPVWCITPPPLDRCIHRFY
                     DTSPVSPSGRYVALTRLPTEDRPPYEGEPAEIVVVDLLTGEWRVVAPTFGYDTQLGAQ
                     AQWGRSDRELYFNDMDVEHWRPFAFRLDPLSGERTELDGTVYMVSPDGAKLASPCLLR
                     TGLTQAGYGVVVPHVALPLNARADEHDGLFITHADTGKRELLVSFAEIFDALPEVRRP
                     LEGESGALYGFHVKWNPQGTRLMFVIRWRRAEGYGRKESSFRRNHVVTMNADGSDIRL
                     AMPAELWARGGHHPNWCPDGEHIIQNLNLFGTGLRFASYRHDGTDLRAFHTEIRGGGH
                     PTLHPDNRHILTDAYTREYAEFGDGTTPLRWIDIESGQETMLVRMRTVPLVEGPMNSL
                     RVDPHPAWDGSFRRIVFNACPDGRRKIFVADLTCSV"
BASE COUNT         2066 a         3600 c         3365 g         2202 t
ORIGIN      
        1 gcgccgccag gatcgcctcc accacctgcg caccgagaat gcgttgtccc gcttcgttga
       61 gatgcacgcc gtcgccggag aatagttcgg ggcggtcgat gacgcgggag aacaggtcgt
      121 tcaccggcac tccgcgtgcg gcggcgagtt ccgcggcgag ccggttccgt tcgcgaaccc
      181 gtgccgttct ggggtcgggg gcccccgatc catccgtgcg caggaaaagc gtggaggaag
      241 cccagatgag cctgctttgt gggctctgtt tcaggatgaa atccaggacg cgggccagcc
      301 cggccgcata ctccgcttca ccgtagccga acccgtgcag gccgttgttg aagtggatca
      361 cggcgaaccg gtagtcgtcc agcaggagtg cgagctcctt ctccattgcc gcgtcgcaga
      421 cgctggtgga ggtggtcaac cgagcacaga ggaacctgcc gttgagcgcg tcgcgcacgt
      481 ggtcgtagta ggagcgggtg atggaatcgc ccacaaggag cacgcggggc gtggtgtcgt
      541 catcggcgcc cataacccag atatcacgcc attcgatgcg ttcgtggatt gtcatggtgt
      601 gcgcttctcc tttcccgata ccgagtgcgg tccggcgcgt gctgctctga cccggcagtg
      661 tggcacaggc gcgtcaatcc agacgataga tagaacaacg cgcggaacac aaccggtcat
      721 cgagtggcgt ccgcgtgcgg acggtgtatc gagatgaccg ggccggtcgg acaggtgcga
      781 tgcgcacggc aggattcgaa agaagaagaa cgcgtgtctc gcccggccct gcgagccagg
      841 ctgtgtggtg tcgcccctgg caacgaaccg cgtgccggtg cgtctcgata cagcgaactg
      901 gcgcttcggc gggcttcctg cgcgcccttc gacaggctca gggaccgcgt gggcctcggc
      961 gcagcgagag gaggctcagc gaccagttcg ccactcgacg accggcagag gccgcaggtt
     1021 tgaagggcgg agcgcttgac cggaaggacc cgacgaagcg tgtccctccc tccgcaccga
     1081 aaaaaaaaac ggggcaggct atcgagcccg ccctgggcgg accagcctgc cccatcaccc
     1141 ggtcaggaat tcgagcgatc tactcgttga acaggtattt gagcagcttc ttccactcga
     1201 ggaaatcggg aatgacgatg tcggcgcctg ccttgatgag ccgctgacgt ttgtgcgcgt
     1261 tcaggccgcg ccgtgctacc tcatcagacg ccacgcccac cgcgatcgcg ccgttgtcct
     1321 tcgcgttgcg aatctccacg ggaccatccc ccaccaccat cagttccgca ccgtgcaggt
     1381 tgttctggga gataatgtcc ttcatcactt tgtctttgga gtagtcatcc acgttcctga
     1441 tagcgcccca gatgccaccc gtggcccact gatcaatctg gcacgcgtga gcttcgttgc
     1501 gcacgtcctc ctgatcagta ccactggcga ggtagatacg cacgccgcga tcgtacagcg
     1561 ccttgaggaa ctcgcgcgat ccgaacatcg tcacgtcttc cagcgtcttc tcgccgcgtt
     1621 ccagcgccgc gatgcgctcg cgcacgggtt tcatgagggc ctcgttgtaa atagccttgt
     1681 agccccacgc gtcgaggatt ttgtccttgg gcacgaggcc gaactcctcg accatgtgca
     1741 cgagcccttc catctggaag aaggtctgga taccggtagt ctcgtcaatg aacttcttgc
     1801 accgctcgac gatttcgggt gtggcggggt ggtcgccccg gatcgcatcc accatgacgg
     1861 gcagcatgat atccggccag ccctcgcgca gcacgctcac cgtgccgtcg aaatcgaaca
     1921 cggcgtgttt gatgtggccg cggtcgatat ccgggttcac gatctcgatc accgaaccgg
     1981 ggagatacgt cggcgtttca gggaccggat acaccttgtt gacgatcgtc tcggaatgtt
     2041 tgcgcagcat gaacagctcc atgaggaaaa gggtttctcg ccagcagggg ccgcgaagag
     2101 gactctggcc gttattgcgg ctatcgtgaa aaccacggca cgcgcggttg gaacacacta
     2161 ctcaaatttc cggaattccc ctctccggac aaaccttccg gcgttctgcg tcagccannn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gcaggaccga acacatgaac
     2341 gacccctccc accgtgaggg tgagcaggtc ggtcgggtca gtcccgatgc ggatagggct
     2401 gccaccccgt ccgacgtcac ggttcatgcg gtcgccccac acgcgactca gctccgggag
     2461 ctgtttcacc aggcggccat agccatcgaa aaagagctgc gcgtcgcgcg aagtgttcgt
     2521 ccagatgagc cggtggcccg ctttgcccca ctttacgggc gtcgcgccct gcccgacgta
     2581 atccggctga atagtccaca agcgttcacc acgcccgctg aagagcgtga gaatcccgta
     2641 gttaccccat ctcgtaaccg ccagcacttc ggtgcccggc aggtcgggcc gcaacttgcc
     2701 gatactccgt ccctgcgcat gcccgattcg gtgcaccacc cgggtggcgc cggtaagggc
     2761 atccaccacg tacacaccgg cgctgcctgc gcacaggaac gcgatcgggt cgtcctccgc
     2821 ttcgccggtg aggttgccca tcacgacggc gtcgtagtgg ccggcgccac ggatctgttg
     2881 catttcgtcc gcgcggtcat gcgtccacag aatggtgccg tcgccgcggt atagatagcc
     2941 gccggccagc atctcttcgc ggtcgtctcc gtccacgtcg aagaaatcca gcgcccagtg
     3001 atgaccgtag tgtgcaccgg tctggacgtg ctcccagagc aggttcaggc cccggtcgta
     3061 cgcccagagc cggattccgc cgccaccgtg gtcatcccgc cactcacgca gcacaatatc
     3121 agtgccgtcg ctgttcgcaa gcgaggcact tcgcggtgac cagtcaaaaa agcgctgcgt
     3181 ggggacgttg ggcggcagtt cagcgcgggc aacgatcttt cccgttcgcc cttcaatcac
     3241 caccatttcg ttttcgaccg tgagcacgct ctccccgccg gcgatgtcct tcgcgcggcg
     3301 ttcttcccgt ttgcccgcga agccatagat gagccgcccc gcagggccaa aatcccgcga
     3361 gaagacgcac cgaacgatcc tttccggaac ttcccagatg aggccacctt caggcgtgag
     3421 cgcgcggagc tgcttgcctt ccacaaggat gtcgaagcgg cccggttcgc tgaagtccct
     3481 gaacaccgga cttccgccga gatcggcagt gccatacgtg cgcacaaggg ttgcgtcagg
     3541 aatgccggcg ccccgttcgg caacttctct ttcgaaacgc gcgtgccgtg attcatcgcg
     3601 gcggcgctgc ccgggggtct gagatacttc gaaccgccgc aggcgtgagg agttggccga
     3661 tcggaaaccc acctttcccg cgtgatacac ggtgtcgcac acgtgcatgt ccacgccgag
     3721 ttctggtgac gtgcatcgga tggagtcacc gtcgaggcgg acttccagcg tcacgtagtc
     3781 ctgcggggcg gcgatggcct gttccgcaag cacggcaaac tcgtcatcgt tccgtcggag
     3841 cagcaccacg cggcgtttcc cctggatgcc gaacaggtag tacgcgcgcg aggtctgcac
     3901 gcggaacacg atgccggcca gcgcttcggt ctgaaccggg ctgtcatcgt tcgggagagc
     3961 gttcgcagcg agtggtttca cctcggcaac gatggtgccg tcgcgcaggt cgactttccg
     4021 cgcaacgatc gcgcgctcat ggcgttcacc ttcggcacac tcgagcgccg gtcttccggc
     4081 gtcgtccacg caggcaagcc cggcaccgac gtcaaacccg ccccacggcg aaagcggcgt
     4141 ttcccatgtg gagaacagcc cgcgctccgc ttccggaacc ggttccatcg gccggaggac
     4201 ggggccgaac gggattccgg aaagatcgag ccggacaagg acagccattt cgggttctgc
     4261 gcgtttcatt cgctccccca gcacgttaat ggttcagttg agccggtcgc cgagccaccc
     4321 gtgctcgacg cggtcggcaa gttggggtga tacctcagcg atgcgctgat cggcgttgcg
     4381 tgcgcgtttc agcttgccga cgccgcgcac ttggcagttc agggcgccca ccagagtgcc
     4441 tgccatggcg gctttgagcg gattgcccgt gcggagataa tgccagaacg tgccggtgct
     4501 gaacgcgtcc ccgcacccgg tggcatccac gaagttcttc acggtgaccg ccgggataat
     4561 gcagccgtac tcgatcccct cacgacggtg tgccagcgcc acgcccgccg ggcccatggt
     4621 gatgacgacg gccgccgcac cggcggccag caccgtctgc gcggcgtgga ggaattcctt
     4681 gggcgatgag ggcgaggtgt gcaggacttt ctcgcattcg aattcgttca tctgcacgaa
     4741 atcgaattgc tcgacccatt ccggccactc gacgaaatcg agatactgct tcttcccgtc
     4801 ggcgtcgaac cgcaccatga tgttgtgcat gtcgaggtag agcggcgcct tcgtctgctt
     4861 ccggagagcc tgcaacgtcg gaagatccag ctccgtgccg ttcacaaagt tgatcaggat
     4921 tccgtcagcg gcgggagtgg atgcgtctcc attcactgcc gagatgatgc gcggggcgct
     4981 gaaaggcggc atcatgtggc ggacgatttc ctcgcggtag ttggacccgt ggtagatcag
     5041 ttttgcgtgg gtgcacggcg tgtcggtacg gaggagctgg ctcgtatcca catggtccat
     5101 gccggccatg agggcgagca cctgatcgta ccggtcgaac ccgacgttgg agacgggcag
     5161 gacgacgccg tcctcgatga aggacgacag cgcggctacg ttatacacaa tgccgccgaa
     5221 cgactcgatg tgctggccgt cgtgcgtgat gatttcgtcg tagacaatgg cgcccagaat
     5281 ggcgattcgc gttggcaaga gagacccttt tccatgtgga agcagtcagc gaagggagac
     5341 aaaaagatac gggagagggg ccagataggc aaagattggg gggaggatgc ggacgccact
     5401 cagtctgcag gaaggtaggg cggcattggt ttcgtgcgca accgcgcatg cagggcgccg
     5461 caacaccaca tcccacagga ggcccaacga gatcgcggtg gtctggggac gaaccgccct
     5521 aaccggttca tcatctgatg cccctggagg gacccgcttc gtcgggtcca caccatttcc
     5581 cctctccggc aggcttcctc ggggttgcgc cagcccggac cacccgcgcg agttggacag
     5641 tgcggtcttc actattctcg tagtgaagga gggatgtctt cttctcccgg caaccagccg
     5701 atcgcttcgc gcatagcctc cctgccgttc atttccagga gctcgcgcca gtagcgccct
     5761 ttgggcagat tggtaacgaa ggtcgttaag aacgcctgtc gccccgccac gtcgggtggc
     5821 catgtgagag cgcaaagctc ccaggcttgc ctcgcattgc cggtgtatat caggctcaac
     5881 atatcgtgcc tcaaaccgtc ctccaaatag gagtacgctc ttgctgagtc ggcaagtgtt
     5941 tccaattccc tccgggtcat gggaggcttc cgcatcaaat tccccgccat gcggtattgc
     6001 ccgttctgga acttcaagat gatgggattg aagatgggcc cggtccatac acgcccgtag
     6061 gcgtaactgg cgtcgtccac ttcaatctcc agcgcggggt cggcatcgag gttcacgaac
     6121 cggctgaaat gcgcgtcatc ggaatagaca gattggatgt gccgaaactg cgaaccaagc
     6181 tcgaaaaagt caagcgagtg gcagcaatgc atgcctcctg aataaccggt gagaacgagg
     6241 tccggtattc catcccccgt aacgtccctg ccgatgagtg tagttgtgca ggtgtctcct
     6301 tcgcctccat caacacatgc gatctcgatg cggccgctat ggccggcgaa aacccgtgtt
     6361 ccgttgcgcg tgatctcaac agatcccccg tcgtcgtata tgccgtaccc atgtccgtat
     6421 atcttcacca cgtagttgcg gaaggacttc cgtgctctca gttcccccgt tggaacgaat
     6481 ttctgattct cttccaccct ctgtatcgcg gattcgaatt gacgtggtcc cgaatacctc
     6541 cggaaccctc cccaccagtc gaggactgga gaggcgtaat catcggtcac gggcacattt
     6601 cccgaatccg cgacgcccgc cagaagcata gtcaggcccc ccaacaagca caattgaagc
     6661 tgcttcatgg ttctcctctc ccagtattcg caaatcgtca agccaccttg gacgcaggtc
     6721 gccctacctg gctcatcacc tggtgcctct ggaggggccc gctcttcttt gcatcgagcc
     6781 gtgccgtgca gggcggaggt cacgacgaag cgtgacccac cagcccggcg gaccggagtg
     6841 tttttgcgaa tggacgtgcg gcggcctgtc tcgctacaag ggttcagtcc cctcgttcag
     6901 agtcgcggga cacgcctgat acacgaatat ccgatcgcgg tggcttccgg tcgaaccttg
     6961 aaaaaggtcc atcgttattg aagattcggc tccaaacttg aataccaggt tctgcagccg
     7021 cagccgagtt accccctcac ccgggcgcgt cgccccagtc gggaacctcg agccactcgc
     7081 catcccgcag cgcggatttg tgcgccatcg cgcccatggc catgtagcgt gcggcctccc
     7141 acgcgttgat gacgggttgc cggttctcgg ccacggcggt gacgaactcg tgcacaaggt
     7201 acggatggct gccaccgtgg ccggtgggcg tgaagtccag atgcgccagt tcgctctccg
     7261 gcgtgccgcg atactccacc cgcttgaacg cgttcaccac ttcggagggc agcgggtccc
     7321 gcatctcctc atcggtcggg gtctggcagg tgggcggagg cacgcgtccc gcctgcgttc
     7381 gcccgttgcg ccaccacatg ccgttgctga aggtgcctgc cgtgccggcg atgcggaacg
     7441 tttcggcatc gtccccgaaa tatcccgggg cttcacgaaa ctcgcagatc cggcacgtgg
     7501 cgccattgct catccggtag aatgcgacaa cgttggagaa ctcctgccgc gcgaaatacg
     7561 ggtcacccgt gcggttgcgg aaaccgatcg cgcttgcctt cacggcgtgc gcgcgcatca
     7621 cgtgcaccgg tccgcttacc gaatgcgtgg ggtacgacat ggggcttccc ttcggttccg
     7681 ttccgcttcg tgtcgtgagc actgtgcccc gcggtaccgc gctcgggttt gccgcccggc
     7741 ttgccgccac gtcgcgcagg ttgcattgcg cgtcgacatc atggatgtat tcaccctccg
     7801 tgtacacgaa cgttccgaac tcgccccggt cagccatgcg gcggcagaac atcgtcttcg
     7861 ggcggtagac cgtggtttcg cccagcatgt accgcatacc cgttttctcg catgtgcgga
     7921 tgatccggtc gcaccactcg agaatctcgt cgaaatcggg caatgagatc accggaaccg
     7981 cgctgtaaac gtgtttgccc gcttccatcg cggcgacgca ctgcggcgcg tggagctgcg
     8041 gctgcgtgat gatggccagc gcgtcaaggt cggcagccag gatgtcgtcc agcgatgcaa
     8101 acgtatcctt cgggtcgaac ttgtcacggt agaacgggtc gttgacgaat ttcgcgaggg
     8161 aggccgggtt cagatcgcag aacgcaattc tgtccaccgc ctgatggctc ttgaaaagcg
     8221 gcgcgaacgc actgccaaac gaaccaagac ccacgagccc gagactgatc cccacgcgtt
     8281 cctccttctg cgttccgtgt tgcctgctga atgaccctgc gaattgcgcg cgagaactac
     8341 cgttcggcgt cccgccgatt cagataccta ccgaaatgcc ggtcgagctt cgaggtgtgg
     8401 ttcaccaccc agtcaatcag aaggatctgc gcatggaaaa ggaggaaatt cctgcgcgtg
     8461 cggaagttct tgcgaatctc ctccttgaat ttcccgaacc tgtccacaaa gcgctgatgt
     8521 tcggcaacct gcatcggcag gaacgggtag tcatactcgg ccatcagctt ttcttccgcc
     8581 gtgaaatgct cggccacgta attgtcgagg aacgagatcg cggtccggat ttcggaaagg
     8641 ttgcgcgtct tctggattgc ctgcaccagc cccttcacaa aatcgaacag ggcccggtgc
     8701 tgctcatcta tttcctcgca gccaacggca actgccgggt tccactcgat catcaggtca
     8761 aattcgccgc tgctttcgtg aacccccgtt tccatgaagc gttcgcaccg cgcgcggtag
     8821 acctgtgcgg cggtatcgtc gagtgcttct gccagacact gggcaagcag ctccgtggcg
     8881 gcgggaacct ctttcatgtg ataccgcgcc aacgcctcct caaacagccc cttggttctc
     8941 cgtttcgcct cccgcaggag gggcggatcg gcatcaaata cctcatacac agactgcggc
     9001 tgttccttcc ctttcacctt cacgcggtcg acaaaccgcg tgtcgtggtg gccgatattt
     9061 cgcgctccgt agtacgtgtg ctcgctgatc aacaacggtg tcccgtaact tttcgtgagc
     9121 gtctcgatgc gcgcggcgag gttgacggca tcgctgataa cggtgctttc gagatggctg
     9181 cgtccgccaa ccactccgag catcgtaagc ccggtgttga gcccgatgcc gacccgtatc
     9241 ggagcctgcc ctctctttct cttcagggca ttgttgaacc cgttgagcct ctgcagcatc
     9301 tcgagggcgc aatggaccgc gtcatccgga ctggtgggaa acagcgccat gacggcatcg
     9361 cccatgtact tgtcaatgat gccgttgtgc cgaacgaaca ccgattccat acgccggaaa
     9421 tatgcgttga tgaagtcgaa gttctcggcc gggctcattc gctcggaaag cggggtgaag
     9481 tccctgatat ccgagaagag aatggtcatc gtcttttcaa catggtctcc gggacgaaca
     9541 tcggtaacgc agcttttgcc cagcaacccg aaggcctcgg agggaatgaa acggcggata
     9601 gcttcctgca tctcgccgtc cagaacatcc cgccgtgcga cgcgtttttt tcgcgccagt
     9661 tgtgtaaccg ctttccgttc aggcatgctc gatccctccc tcgttgtctg cgccacaact
     9721 caccactctg caaacacccg ccagatggcg caaaaaagag tcccggaaca ccggtcccgg
     9781 cacgttgccg gcaagcaacg ctgctgcggc gcattcatcg cgcaaaatat acgcgaattc
     9841 cagaactgga caagcggaac agactgacgt ggtcagaatt cgggcgttac acgctgcatg
     9901 tcaggtctgc cacgaatatc ttccgccgcc catccggaca cgcgttgaac acaatccggc
     9961 gaaaggaccc gtcccatgcc gggtgcgggt ccacccgcag ggaattcatc ggcccttcaa
    10021 ccagcggcac cgtgcgcatg cgcacaagca tcgtctcctg cccgctttca atatcaatcc
    10081 accgcagcgg cgtggtgccg tccccgaatt ccgcgtactc ccgcgtgtac gcatcggtga
    10141 gaatgtgccg gttgtccgga tgcagcgtgg gatggccgcc gccacggatt tccgtgtgaa
    10201 acgcacggag gtcggtgccg tcatggcggt acgaggcgaa ccgcagcccg gttccgaaca
    10261 ggttgaggtt ctggatgatg tgctccccgt cgggacacca gttcgggtgg tggccgcccc
    10321 gcgcccatag ttccgcaggc atggcgagtc gaatgtcact cccatcggcg ttcatggtga
    10381 ccacgtggtt tctcctgaat gagctctcct tgcgcccgta cccttccgca cgccgccagc
    10441 ggatgacgaa catcagccgc gtgccctgcg gattccattt gacatgaaaa ccgtacaggg
    10501 cgccggattc cccttcgaga ggccgcctca cctcaggaag cgcatcgaaa atctccgcga
    10561 aggaaaccag cagttcgcgt tttccggtgt ccgcatgggt gatgaacagt ccgtcgtgtt
    10621 cgtccgcacg ggcgttcaac ggcaacgcga catgcggtac gactacgccg tatcccgcct
    10681 gggtgaggcc ggtgcggaga aggcacgggc tcgccagctt cgcaccgtcg ggcgagacca
    10741 tgtagaccgt gccgtcgagc tccgtccttt cgcccgagag tggatccagc cggaaggcga
    10801 acggccgcca gtgctccacg tccatgtcgt tgaaatacag ctcccggtcg ctgcgccccc
    10861 actgtgcctg cgccccaagt tgcgtgtcgt atccgaacgt gggcgcgacc accctccact
    10921 ccccggtgag cagatctacc accacgattt ccgccggttc cccctcgtac ggcggcctgt
    10981 cctccgtcgg caggcgggta agcgctacat acctgccgga cgggctgacc gggctcgtgt
    11041 catagaaccg gtgaatgcac cggtcgagag gaggcggtgt gatgcaccag accggtaccg
    11101 cagggctgaa tgcctggtac gtttcgaaac ggtcggatat cggcaccggt gaagtgagtg
    11161 gcaggtctct catggtgact cccttcgctt cacgcgttcc gagcgctctc acgcgtccac
    11221 ctcggccaat ccagggatcg cgcctttcca gatatcgacc ggaacatcgt ttgcctgcga
    11281 tggccccggc gtggaccgcc cggcggatac ctgctcttcc agtgccctca caa
//
LOCUS       DAOSOO010000094        11262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5749_length_11262_cov_5.470242, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000094 DAOSOO010000000
VERSION     DAOSOO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_11262_cov_5.470242"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLT86_08050"
     CDS             <1..576
                     /locus_tag="PLT86_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36602.1"
                     /translation="RTLLLGSKVPNVPNEQSGGQPVYYVKDADRPPVMEVPGGLGYNL
                     KRPMEFYWDRHLAVFERRDINRVELVTPERTVAMSADTLANWRFESPLSGGVKRWQVN
                     GIVANLDMARVKQFVERPVAATGLDRPRLSVRLYHDNELKASVDFGAPHGGLVFAKGS
                     NNNSPVLVDQQTMALFTLSVDDLKELPIPPQ"
     gene            711..1697
                     /locus_tag="PLT86_08055"
     CDS             711..1697
                     /locus_tag="PLT86_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36603.1"
                     /translation="MSWVHRVSLAALVGIFFCAGTCPGFVEVAARVDTGAVLIGDVIT
                     YEILVTYDDTILVEKLPHGVNLTAFHIRDYRAIPKTKLANDLWQEGGQYRISVYRPGL
                     YVIPPVPVRYHGRSGQPGELITQPITVEVKSMGVTEEDTLRGIKGPKSVPVRFPIWIW
                     GMPAVMILAGVAVLFAVKRRGRKSTMAVEPPATVVLDEVAEFDRIPAADLVRSGDLKL
                     LYTLVSDAMRRYLARRYGVNAMELTLHELALSLESVGADANDVGDMISFLERCDLVKF
                     ARFIPAHDEVETLIERAKNLVRQTQVSTVAAVSETSPDNAADDRDGEGSTAV"
     gene            1694..2704
                     /locus_tag="PLT86_08060"
     CDS             1694..2704
                     /locus_tag="PLT86_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT36604.1"
                     /translation="MTFSLASPWWLLLLIVLPLQVWWHTRGTHFTNAVLRLPSATSLR
                     QFRPSTRVRLRHVVLVLQILGELILIVALARPRSGKVEQRVISEGVDIVLALDVSGSM
                     QAFDMGNRERIDVAKEVTSQFIDSRASDRLGMVVFAGQSYTQCPLTVDYGILKNLLAQ
                     VSIRMNETIPDGTAIGMALATATNRLRKSTAPSKVIVLLTDGSNNAGIIDPLTAAEAA
                     AQLGIRVYAIAVGVTGRAPIRVRDPLFGERIEFMEDSINEDVLREIAAITGGRFFRAT
                     SQTALQEIYREIGMMEKTEVATLQIPRFAELTFVFHLIPLGLGLLILGSFLRFTVFRT
                     IP"
     gene            2724..3761
                     /locus_tag="PLT86_08065"
     CDS             2724..3761
                     /locus_tag="PLT86_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT36605.1"
                     /translation="MDILISLVTQLWRDPEWLWGLLLVPIVPLAYFVLEWRARSLITR
                     LGNLDVLTRSAIEVSTRLRMVRLCLFSGAIAFGFAAAARPQWGSSTEIVHSTGLDIVL
                     LLDTSLSMLAEDLKPNRLESAKLEVATFLDKLKSDRVALVPFAGTAFIQCPLTLDYSA
                     LRLFLSDITPYTVNEEGTQIGTAIRTGLKAFGEGDDRSRVMVLITDGEDQGSNPVEAA
                     QMAADEGVRIYAIGIGSPEGELIPDTGIDGRPDFLRDDQGSVVKTKLDEEALREIAIE
                     TGGEYYRASPGGRELEAILESISDMERTRQGEFQTVARIDRYQTPLACSVLLFLVIPF
                     VPERAGTRRRS"
     gene            3758..4426
                     /locus_tag="PLT86_08070"
     CDS             3758..4426
                     /locus_tag="PLT86_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36606.1"
                     /translation="MTKVSAYVLVLAVWLFGCELSDVLVQRSVARKVQEGNALYLRGE
                     LNEALIRYRDAETDAPEEGRIKFNIGNVFYRQAQFGEAEETYSDAARRGDALSRADAW
                     YNLGNACFKQGRFDKAVESYQKALEFNPSDRDAKFNLELVQKMLNRAASEAASTPAGY
                     RRPSNWARDRMKEAEALAQRGKYGEAQRVISRTFQAEPSCVGEFGEFAERVNALRTIF
                     GEGR"
     gene            4423..4947
                     /locus_tag="PLT86_08075"
     CDS             4423..4947
                     /locus_tag="PLT86_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36607.1"
                     /translation="MNGVLFFAVALALGGGTYATGQTVSGLPPELLVARAAQAVATIA
                     FDRSLEEQALRDLAAALRQDPRNRHAKALVDLIQKRRQEREKQEQQRGQQQLRNQRPE
                     PQPQVAQQQPGTKQKVSSNAPDRTETNRRAPREEGNRLTREQAQQLLDALEQQDRRPT
                     QRLTVRNPRQGPRW"
     gene            4977..6758
                     /locus_tag="PLT86_08080"
     CDS             4977..6758
                     /locus_tag="PLT86_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOT36608.1"
                     /translation="MCNKILTGVTTLLLLASMGRAEVQVFARVDRNQAQVGEQIILTV
                     TVVSQKPLSVQVPPTLRISGVSCSQVRGPATSEEMSISGGRLLRRSTAEYAYFLTAER
                     AGEIEIPAIPVAVEGTQFLTQPIRLTVGKSPVLEAGGPVQVRVNVSRTTVYQGEQIIV
                     EYLLYEEAVASSRIQNRQLVRDPSFLNFWAETLEDIRRQQIPTRRETVNGVAYNVLPI
                     LRYVVFPTRAGTLTIPSITVSLVAALPTGARTIWGEPVLQPRQISVSSPERTIESRAL
                     PANPPAGFSGAVGSFSIVSEVDKNEVAEGDPITWKVIIEGDGNVKSVGDLRIPDLTDF
                     RAYDPKTTASFTLAQGAVRGKLVYERVVMPLHAGISQIPGTTFTFFDPRTRQYRTIAV
                     EAKKITVTANRAAVARTPGGGQMLVKEVGSDIRYIQPDVNSLDDESWRLTDSPFYWVS
                     YGLPVAWVLLAFGVRARRIRMGANPARMRALGAAGEARRRLKAARLAFEKNDVHGGIQ
                     AVANALTELVAAAANTTAAGLTSSRISEVLSEKEVPEDLRKEVLATLAQCDEIRYAPT
                     SASVDDAKAIYEKGLDLVRQLLRYLVP"
     gene            6755..7525
                     /locus_tag="PLT86_08085"
     CDS             6755..7525
                     /locus_tag="PLT86_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36609.1"
                     /translation="MKRTVASVFLLVLILAVARSSGASNPGPARLFNEGNAAYRQGRY
                     EDAIAAYNKVLAGGVRSSAVYYNLGNSYYRTGKLGFAILNYERSLRLDPANDDTRHNL
                     RFVSSLVQDRVLVSSFELKFQEKLSQFLGYTNPNLVAVIVLLAVFVSGGIGGLWLLGR
                     FRTRIAMVIFVGCCVLAALGASVMAGQKWAADEGRTGIVLADQTNARFEPSETSRVVF
                     VVHEGTKVWLIRRDGGWAFVRLANGLRGWVQQGAFEMI"
     gene            7537..8424
                     /locus_tag="PLT86_08090"
     CDS             7537..8424
                     /locus_tag="PLT86_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT36610.1"
                     /translation="MASRLFAWGAALVVLAGQVWAQPSYLVPLLNEIERTNPASFYTV
                     TFRLYERVGAGVYSPEDLRFLSSALLRRAEASPELLPVVLPLLGQMNVPETVPPIMTH
                     ARSSDQAVRTAAYQALGWMGDERALPLLTARNRQERPAASAEERFMIEYATRSIELKA
                     RLRRMSSGDQFGLVRTTLLTEPNWLVRGDIARMLSKRAGADVWAVLFDSYERWATTPQ
                     YVQMIGEVLGQRYRFDPTGYIAAVRRRPPETRLYALERISDFPHISDMGAIMDISETD
                     SDPIVRERATILLGKLLNR"
     gene            complement(8421..10169)
                     /locus_tag="PLT86_08095"
     CDS             complement(8421..10169)
                     /locus_tag="PLT86_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT36611.1"
                     /translation="MSSCPRPEYPRPDLVRAKWQNLNGRWQFCLDPGLSGVSRGLANP
                     GTNLPQEIVVPFCPESRLSGIANTDFMGGVWYRRTFTIPEDWSGRRIILHFGAVDYLA
                     HVWVNGKLLGSHRGGYSPFSFDMTDALGAGTNVLTVFAEDDTRSPLQPSGKQSIRYES
                     HGCHYTRTTGIWQTVWMEPVPDAHVTGLRFVPDLHQAKVLVEIHASKAAEGARAEITL
                     KAGGENVGSGEGHFIGGVAFVVVPVGSVRLWSPEDPFLYDVSVRMRAVDHAEDAVKSY
                     MGLRTLGTRGRALLLNGQARFQRLVLDQGFYPDGIYTAPTDEDLKRDIEISQAAGFDG
                     ARLHMRVFEPRFLYWADKMGYLAWGEYPNWGLDLSNPASLIRVQEEWLSILRRDVNHP
                     SIVGWCPFNETHQQPPRDPDILRLVYRTTKLFDPARPCIDTSGYVHTECTDVYDSHCY
                     EQDVAKFAANFAGMISGQEVWRNFPKEDAEWAGQPYFVSEYGGIWWNPGKEDGKSWGY
                     GDRPKTEEEFLTRYRGLTEALLNHPMMCGFCYTQLYDVEQEVNGLYTYQREPKFDPAI
                     FREINRQKAAVEKTES"
     gene            complement(10192..>11262)
                     /locus_tag="PLT86_08100"
     CDS             complement(10192..>11262)
                     /locus_tag="PLT86_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT36612.1"
                     /translation="HGVGHLIAHNLIHDLPHCAILFWGNEITIENNEIYSVCLETGDA
                     GAIYTGRDYTFRGNLIRRNFIHHTGGVGMGSMAVYMDDCVSGTTICENIFWDVTRAVF
                     LGGGRDFVVRNNVFVDCHPAIELDSRGTSDHPVWRRMVMGYMKEQYEKMRPSEPPYRL
                     RYPELAAIQPYFSGTTGVPAEGNVITHNVCLGEWVRIDESAAPLVEIHDNFVDVEPPF
                     CDPAYGVFAIEPNSPLLHAGFDPIPVEEIGLVRNEVRTSIPPRVGTKLEHVNREDGDG
                     VRVSAKNLGDTPAEGSLRFRVHRAVEPELCDFPEWKFTLLPGETASSEFSLEGINRPV
                     TIEAHSQVPGVRPSRLTISLET"
BASE COUNT         2371 a         3087 c         3422 g         2382 t
ORIGIN      
        1 cggacactgc ttctcggttc gaaggttcca aatgttccga acgagcaatc cggggggcaa
       61 ccggtgtact acgtcaagga tgcggaccgg ccgcccgtga tggaagttcc gggagggctt
      121 gggtacaacc tgaaacggcc gatggagttc tactgggatc ggcacctcgc agttttcgag
      181 agacgtgaca tcaaccgggt cgaactcgtg actcctgagc ggacggtggc catgtctgct
      241 gatacacttg ccaactggcg gttcgagagc ccgctgtccg gcggagtgaa gcggtggcag
      301 gtgaatggca tcgtggcaaa tctcgacatg gcacgtgtga aacaattcgt tgaaaggccg
      361 gttgccgcca cggggctgga taggcctcgc ctcagtgtcc gcctgtacca cgacaacgag
      421 ttgaaagcgt cagtggattt cggagcaccg cacggtggac ttgtttttgc caaggggagc
      481 aacaacaaca gcccggttct ggtcgatcaa cagacgatgg ctcttttcac gctgagtgtc
      541 gacgatctga aggaactgcc gattccgcct caatgatgtt ctggcaagcg gggactggca
      601 tcagaaggac ggtgccacgg cagacagcag cagtatgcaa cagggggtgc gatctatcgc
      661 gccctctttt gctgaaaaag gtttgcactt actctggcag ggtcatgccg atgtcatggg
      721 ttcaccgggt gagtctggct gcacttgttg ggatattttt ctgcgccggt acctgcccgg
      781 gtttcgtcga agttgctgct cgtgtggata ccggtgccgt cctgatcggg gatgtcatca
      841 cgtacgagat tctggtaacc tacgacgata ccattctggt agagaagctt ccccacgggg
      901 tcaaccttac cgccttccac atcagggatt accgggccat tccaaaaacg aagcttgcca
      961 acgacctgtg gcaagagggt gggcagtacc gcatttcggt gtaccgtccc ggcctttacg
     1021 taattccgcc cgttcccgtg cgataccatg gcaggtccgg gcaaccgggg gagctgatca
     1081 ctcaaccgat cacggtcgaa gtgaaaagca tgggcgtgac cgaggaggat acgttacgtg
     1141 gcatcaaagg gccgaagtcc gttccggttc ggttcccgat ctggatctgg ggtatgcccg
     1201 cggtgatgat tctggcggga gtcgcggtgc tttttgcggt gaagcgtcga ggccgaaaga
     1261 gcaccatggc ggtagaaccc cccgcaaccg tcgttcttga tgaggtcgcg gaattcgatc
     1321 ggattcccgc tgcggatctg gttcggtcag gagatctcaa gctcctctac acgcttgtgt
     1381 ccgatgcaat gcggcggtac ctcgcgagaa gatacggcgt caacgcgatg gagctcacgc
     1441 tccacgaact cgctctgtct ctggaatccg tcggtgcgga cgcgaatgat gttggggaca
     1501 tgatttcgtt cctggagcgg tgtgacctcg tgaaattcgc gcgatttatt cccgcacacg
     1561 acgaggttga aacgcttatc gaacgcgcga agaacctggt tcggcagacg caggtctcaa
     1621 ccgtcgcggc agtgtcggaa acgagtccgg acaacgccgc tgacgataga gacggtgagg
     1681 ggagtacggc tgtatgacct tcagcctcgc cagtccgtgg tggcttctcc ttctcatagt
     1741 cctgccgtta caggtgtggt ggcacacgcg tggcacgcac ttcacgaacg cggtgctgcg
     1801 gcttccttcg gcgacgtcac tcaggcagtt caggccgtca actcgagtca gattgcggca
     1861 cgttgtgctg gtactgcaga ttttgggaga actcatactg attgtggccc ttgcgcgtcc
     1921 ccgatccgga aaagtcgagc agcgtgtcat ttctgagggc gttgacatcg ttctcgcgct
     1981 ggatgtcagt gggagcatgc aagcctttga catgggtaac cgggagcgga tagacgttgc
     2041 caaggaagtg acttctcagt tcatcgattc acgcgcctcc gaccgacttg gaatggtggt
     2101 atttgcgggg cagagttaca cccagtgccc attgacggtg gattacggta tcctgaagaa
     2161 cctcctggcg caggtgagca tccgcatgaa tgagaccatt cccgatggta cggcgatcgg
     2221 gatggcattg gctacggcaa cgaatcgact cagaaagagc actgctccga gcaaggtgat
     2281 cgtcctgctc accgatggga gcaacaacgc ggggatcata gaccctctga ccgctgcgga
     2341 agcggctgca caactgggaa tcagagtgta cgccatcgcg gttggcgtca ccgggcgcgc
     2401 gccgattcgc gttcgcgatc ctcttttcgg ggaacgtatc gaattcatgg aggacagcat
     2461 caacgaggac gttctgcggg agattgccgc tatcacgggc gggcgctttt tccgtgcgac
     2521 gtctcagacg gctttgcagg agatctaccg ggaaatcggc atgatggaga agacggaggt
     2581 ggcaacgctc cagattcctc gttttgcgga gttgaccttt gtctttcatc tgataccctt
     2641 ggggttggga ttgctgatcc tcggctcctt cctgcgcttc actgtgttca ggacgatccc
     2701 atgaagtggt ggagtacgga actgtggaca tcctgataag ccttgttact caactctggc
     2761 gggatccgga atggctttgg gggctgcttc tcgttcccat agttcccctt gcgtatttcg
     2821 tgctggaatg gcgtgcgcgt tcactgatta cccgtctcgg taacctcgac gttctcacac
     2881 gatcggcaat cgaggtcagc acccgtctga ggatggttcg cctgtgtctg ttcagcgggg
     2941 caattgcttt cggctttgcc gcagcggcca ggccacagtg gggatcatca accgaaatcg
     3001 ttcacagcac cgggctggat attgtgcttc tgctcgacac atcgctttca atgctcgcgg
     3061 aggatttgaa acccaaccgg ctggaatcgg cgaaactcga agtggcgacg tttctggaca
     3121 agctcaaatc ggaccgcgtg gccctcgttc cgtttgcggg tacggctttc atccagtgtc
     3181 cgctgacgtt ggactactcc gctttgcgac tttttctcag tgacattacc ccgtacacgg
     3241 tgaacgaaga ggggacacag attggaaccg cgattcgcac cgggttgaaa gcattcggcg
     3301 aaggtgatga cagaagtcgc gtgatggtgc tgatcacgga cggcgaagat cagggaagca
     3361 atcctgtgga agcggcccag atggctgctg atgagggcgt gcggatttac gcgatcggta
     3421 tcggatctcc ggaaggtgaa ctcattccgg atacaggcat agacggccgt ccagatttcc
     3481 tgcgtgatga tcagggaagc gtggtgaaaa cgaaattgga cgaagaagca ctgcgggaaa
     3541 tcgcgatcga gacgggcggc gagtattacc gcgcgagccc gggtgggagg gaactggaag
     3601 ccatactgga aagcataagt gatatggaaa gaacccggca gggtgaattc cagacggttg
     3661 cccgtatcga ccggtaccag actccgctgg catgctctgt tctgttgttc cttgtcattc
     3721 cgtttgttcc agaacgggcg ggaacacgga ggcgatcatg acaaaggtga gcgcgtacgt
     3781 tcttgtactg gcggtctggc tgttcggttg tgaattgagt gacgtgctgg tacagcgttc
     3841 agtcgcccgc aaggtgcagg aaggaaacgc actttatctc cggggagaac tgaacgaagc
     3901 gctgatcagg taccgcgatg cggaaaccga tgcgccggaa gaagggcgta tcaagttcaa
     3961 catcgggaat gtgttctatc gtcaagccca gttcggtgaa gcggaagaga catattcgga
     4021 tgccgcgaga cggggcgacg cattgtcgcg ggcggacgcc tggtacaacc tcggcaacgc
     4081 gtgcttcaaa caggggcgtt ttgacaaggc ggtggaaagt tatcagaagg ctctggagtt
     4141 caacccgtct gacagagacg cgaagttcaa tctcgaactg gttcagaaaa tgctgaaccg
     4201 tgcggcaagc gaagcggcga gtacgccggc cggctaccga cgcccgagca actgggcgag
     4261 ggatcggatg aaagaagcgg aggcgcttgc acagcgaggt aaatatggcg aggctcagcg
     4321 agtgatttca cggacgttcc aggcggaacc ctcatgtgtc ggcgagttcg gggaattcgc
     4381 cgaacgggtg aatgcgctga ggacgatatt cggggaaggc cgatgaacgg cgttttgttc
     4441 tttgcagttg ctctagccct cgggggcggc acttacgcaa caggccagac ggtttctggt
     4501 ctgcctcccg agcttctggt agcaagagcc gcgcaggcgg ttgccaccat tgcgtttgat
     4561 cgctcccttg aggaacaggc gcttcgcgac cttgcagcgg cgcttcggca ggaccctcgc
     4621 aaccgccacg caaaagctct tgtcgatctc atacagaagc gtcgacagga aagggagaaa
     4681 caggagcagc agagagggca acagcagttg cgcaaccaac ggcccgaacc ccagccacag
     4741 gtcgcgcagc aacagcccgg gacgaaacag aaagtgagca gcaatgcgcc cgaccgcacg
     4801 gagactaatc gtcgggcgcc gcgcgaagag ggcaacaggt tgaccagaga acaggcgcaa
     4861 cagctcctcg acgcgcttga gcagcaggat agacgaccaa cgcagagact cacggttcgt
     4921 aacccccggc agggaccacg atggtgaaag tcgtgggttg tgtggtgcga atgcgtatgt
     4981 gcaataagat cctcaccggt gtgacgacac ttttgctcct ggcgtcgatg ggccgcgcgg
     5041 aggtgcaggt gtttgcgcgg gtagaccgga accaggcaca ggtaggcgag cagatcattc
     5101 tcaccgtcac ggtcgtgagt cagaaaccgc tcagcgttca ggtaccccca acactgcgga
     5161 tatcaggtgt cagctgttct caggtgcgcg gcccggcaac ttcagaggaa atgtctattt
     5221 ctggagggcg tcttctgagg cgttcgaccg ccgaatatgc ctattttcta accgcggagc
     5281 gcgcaggcga aatcgagatt ccggcgattc cagtagcagt tgaggggacg cagtttctga
     5341 cccagcccat acgtctgacc gtgggaaaat caccggtact agaagccgga ggaccagttc
     5401 aggtgcgggt gaacgtgagc cgcacaacgg tgtaccaagg tgagcaaatc atcgtggaat
     5461 atctgttgta cgaagaagca gtggcgagca gcagaatcca gaaccgccaa cttgtgcgcg
     5521 acccttcatt cctgaacttc tgggccgaaa cgctggagga tatccgccgt cagcagattc
     5581 ccacccggag ggaaacggtc aacggggttg cgtacaatgt gttgccgatc ctgcgttacg
     5641 ttgtgtttcc cacgcgggcg ggcacactga cgatcccatc aatcaccgtg tcgcttgtgg
     5701 cagccctgcc gaccggagcc aggaccatct ggggcgaacc ggttctgcaa ccgagacaga
     5761 tcagcgtgag ctcgcctgag cgcacgattg aatcacgagc gcttcccgca aaccctcccg
     5821 ccgggttcag cggggccgtc ggatcctttt cgattgtgag cgaggtggac aagaacgaag
     5881 tcgctgaggg agaccccata acctggaagg tgatcataga aggcgacgga aacgtgaaaa
     5941 gcgtgggcga tctgcgcatt ccagatctga ctgattttcg cgcatatgat ccgaaaacta
     6001 ctgcttcttt caccctcgca cagggcgcgg tgcgcggcaa gttggtttat gaacgcgtcg
     6061 tgatgccact tcatgccggt atcagccaga ttccaggcac tacctttacg tttttcgatc
     6121 ccagaacgag gcagtataga accatcgcgg tggaagcaaa gaagatcact gttacggcga
     6181 acagggcagc ggttgcgcgt acgcctgggg gcggacagat gctggtgaag gaagtcggct
     6241 cggatatccg ctacatccaa ccggatgtca attcgttgga cgatgagtca tggcgtctta
     6301 ccgattcccc gttctactgg gtgtcgtacg gccttcctgt ggcgtgggtt cttcttgcat
     6361 ttggcgtacg ggcgcgcaga attcgtatgg gcgcgaaccc ggcacggatg cgtgcgcttg
     6421 gcgccgcggg agaggctcgt cgacgtctga aagctgcacg cttggcgttc gagaaaaacg
     6481 acgttcacgg ggggattcaa gccgttgcga acgcgttgac cgaactggtt gctgcggcgg
     6541 ccaacaccac tgcggcgggc ctcacttcgt cgcgcatatc cgaggtcctc agcgaaaaag
     6601 aagtgccgga agacctcagg aaagaagtct tggcaacgct tgcgcagtgt gatgaaatcc
     6661 gctacgcccc gacttccgcg tcggtcgacg acgcgaaagc gatctacgag aaggggctcg
     6721 accttgtccg ccagttgctg agatacctcg tgccatgaaa aggactgtcg cctccgtttt
     6781 cctactggtt ctgatcctgg ccgtggcgcg ctcatcgggc gcttcaaatc cggggccggc
     6841 ccgtctgttc aacgagggga acgctgcgta ccggcagggg aggtatgagg acgcgatcgc
     6901 cgcgtacaac aaggtgctgg ccggaggagt gcgttcaagc gcagtctatt acaaccttgg
     6961 gaattcttat taccggacgg gcaaactggg gtttgccatt ttgaattatg aaagaagtct
     7021 ccgcctcgac cccgccaatg atgatacccg tcacaacctc agatttgtgt cctcgctcgt
     7081 tcaggaccgt gttctggttt ccagcttcga attgaaattc caggagaagt tgtcgcagtt
     7141 cctcgggtac acaaacccca atttggtggc ggttatcgta cttctggcgg tgttcgtctc
     7201 tggcgggatt ggcgggcttt ggcttcttgg acggttccgc acgcgcatcg caatggttat
     7261 tttcgttggg tgctgcgtgt tggctgcttt gggcgcaagc gtcatggctg gccaaaaatg
     7321 ggcggccgac gaagggcgca ccgggatagt gcttgccgac cagacaaacg cgcgttttga
     7381 accgagcgaa acttcgcggg tggtgttcgt cgttcacgaa ggtaccaaag tctggctcat
     7441 acgacgcgac ggtgggtggg cattcgttcg acttgccaac ggtctgcgcg ggtgggttca
     7501 acagggtgcg ttcgaaatga tatgaaggag tggtgtatgg cttcgcgcct gttcgcatgg
     7561 ggcgcagcgt tggtggttct tgccggccag gtgtgggctc agccttcgta cctggttccg
     7621 ctcctcaacg aaattgaacg aaccaacccg gccagcttct acactgtcac cttcaggctc
     7681 tatgagcgag tcggggcggg tgtttattcg cctgaagacc tgcgtttcct cagcagcgcc
     7741 ttactccgcc gcgcggaggc ctcacctgaa ctgctgcctg tagtcctccc gcttctggga
     7801 cagatgaatg tcccggaaac tgtgccgcca atcatgaccc acgcgcgatc atctgatcag
     7861 gcggtaagga cggccgccta tcaggccctg ggatggatgg gcgacgaaag ggctctccct
     7921 ctcttgacgg cccgtaatcg gcaggaaagg cctgcggcca gcgcggaaga gcggttcatg
     7981 atcgaatacg cgacacgatc gatcgagctt aaagcccgat tgcggcgcat gtcgtccgga
     8041 gaccaatttg ggttggtccg cactaccctg ctcacggaac cgaactggct ggtgcggggt
     8101 gacatagcga ggatgctgtc aaaaagagca ggtgcggatg tctgggcggt cttgttcgac
     8161 tcgtatgaac gttgggcaac tactccgcaa tacgtgcaaa tgattggcga agtgcttggc
     8221 cagcggtaca ggttcgaccc gacgggttac atcgctgcgg tgagacgacg tcctccggag
     8281 acccgcctct acgcgttgga acgcatttcc gacttccctc acatcagcga catgggcgcc
     8341 ataatggaca tctcggaaac cgattcggat ccgatagtgc gcgagcgcgc cacaatcctg
     8401 cttggaaaac tgctgaaccg ttaggattca gttttttcca ccgcagcctt ctgccgattt
     8461 atctccctga atatcgcggg atcgaacttc ggttcgcgct ggtaggtgta gagcccgttg
     8521 acctcctgct ccacatcgta cagctgggtg taacaaaaac cacacatcat cgggtgattc
     8581 agcagagctt ccgtaagacc cctgtaacgt gtgaggaact cttcttccgt ttttggccgg
     8641 tcaccgtatc cccacgactt gccgtcctct tttccggggt tccaccagat cccgccgtac
     8701 tcgctcacga agtacggctg gcccgcccat tcagcatcct ctttggggaa gttgcgccat
     8761 acttcctgcc ctgaaatcat tcccgcgaaa ttggccgcaa acttcgcaac gtcctgttca
     8821 taacaatgtg aatcgtatac atctgtgcat tccgtgtgaa cgtaaccgga agtatcgatg
     8881 cacggcctag cggggtcaaa aagcttggtg gttctgtaca ccagccgcag tatgtccgga
     8941 tccctgggtg gttgctggtg cgtttcgttg aacggacacc acccgacgat agaagggtga
     9001 ttcacgtcgc ggcgaaggat cgaaagccac tcctcctgta cgcgtatcag ggacgccggg
     9061 ttcgaaagat caagacccca gttggggtac tcgccccatg cgaggtatcc cattttgtct
     9121 gcccagtaaa ggaatcgtgg ctcgaagact cgcatatgaa gacgggcgcc atcaaatccg
     9181 gcggcctgcg atatctcgat gtcgcgcttg aggtcctcgt ccgtcggcgc ggtgtagatg
     9241 ccgtccgggt agaagccctg gtccagaacc agccgctgaa atcgagcttg cccgttgagc
     9301 aacagcgccc ttccgcgtgt gccgagagtg cgcaagccca tgtagctttt cacggcgtct
     9361 tccgcgtggt ccaccgcgcg catacggacg gacacgtcgt agaggaaggg gtcctccgga
     9421 gaccacaacc tgacgctgcc cacagggacc acgacgaacg ccacacctcc gataaagtgg
     9481 ccttcaccgg agccaacgtt ctcgccaccg gccttcagag ttatttccgc gcgcgctccc
     9541 tctgctgctt tagaagcgtg gatttctacc agcactttcg cctgatgaag gtctgggaca
     9601 aaacggagtc ccgtcacgtg cgcgtccgga accggctcca tccacacggt ttgccagatc
     9661 cccgtggtgc gcgtgtaatg gcacccatgc gactcatacc gaattgactg tttgcccgaa
     9721 ggctgaaggg gcgaacgcgt gtcgtcctcc gcgaacacgg tgagtacgtt cgttccggca
     9781 ccaagcgcgt cagtcatgtc aaacgagaaa ggcgagtacc cgccccggtg cgaacctaga
     9841 agcttcccgt tgacccacac atgcgccagg taatccactg cgccgaaatg caggatgatc
     9901 cttctgccgc tccagtcttc cgggatcgtg aacgtgcgac ggtaccacac gccacccatg
     9961 aaatcagtat tagcgattcc cgagagtctg ctttcagggc agaacggtac aacgatctcc
    10021 tgggggagat tggtgccggg gtttgccaga ccgcgcgaaa cgccactgag tccgggatca
    10081 agacagaact gccacctgcc gttcaagttt tgccactttg cgcggaccag gtccgggcga
    10141 ggatactcgg ggcggggaca agaagacatc ggttactcct gagaaattga gtcaagtctc
    10201 cagtgaaatc gtcaaacgtg aaggtcgaac gccaggcact tgtgaatgcg cctcgatcgt
    10261 gactgggcga ttgataccct cgagggaaaa ctcggaagat gctgtttcgc cgggcagaag
    10321 agtgaatttc cattccggaa aatcgcatag ctcaggttcc acggcacggt gaacccgaaa
    10381 acgaagtgat ccctctgcgg gggtgtctcc caggttcttc gccgaaaccc tgacgccatc
    10441 tccgtcttcg cgattaacat gttccagctt cgttccgact cggggcggaa tcgaagtacg
    10501 aacttcgtta cgaacgagac cgatttcctc caccggtata gggtcaaacc ccgcgtgaag
    10561 cagaggcgag ttcggctcaa tcgcgaacac tccgtacgcc gggtcgcaaa acgggggctc
    10621 cacgtcgacg aaattgtcgt gaatctcaac gagcggtgcg gcgctttcgt ctattcgtac
    10681 ccattcaccc agacacacgt tgtgcgtgat gacgttccct tccgcgggaa caccggtggt
    10741 tcccgagaaa tacggttgga tcgcggcgag ttcgggatat cgaaggcggt agggaggctc
    10801 cgaaggtcgc attttctcgt attgctcctt catgtatccc atgaccatgc ggcgccatac
    10861 cgggtgatcg cttgtccccc tgctgtcaag ttcgattgca gggtggcaat ccacaaatac
    10921 gttgttgcgt acgacgaaat ctcgtccacc gccgagaaaa acggcccggg taacgtccca
    10981 gaagatgttt tcgcaaattg tagtgccgct tacgcaatcg tccatgtaca cggccattga
    11041 acccatgccc acaccaccgg tgtggtggat gaaattgcgc cggatcaaat ttccacggaa
    11101 cgtgtaatcc cttccggtgt agatggcgcc cgcatccccc gtttcgaggc acacggaata
    11161 gatctcgttg ttttctatgg taatctcatt tccccagaaa aggatcgcgc agtggggcag
    11221 gtcatgaatc agattgtgtg cgatcaggtg cccgacaccg tg
//
LOCUS       DAOSOO010000095        11182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5802_length_11182_cov_6.230880, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000095 DAOSOO010000000
VERSION     DAOSOO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_11182_cov_6.230880"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1151
                     /locus_tag="PLT86_08105"
     CDS             219..1151
                     /locus_tag="PLT86_08105"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT36613.1"
                     /translation="MKNFKPAYGIVPPLITPFNEDLSIDWYAYDRLIDWHIERNVTGL
                     FVVCGSSEYWKLTEDEAIQMAKAAVKRANGRIHILAGSTNYPDDDLTKNINMTKRMAD
                     TGVDGCFITTPRTIPPQDDLMLDYHMKIHDAVNYPLYAYEMPGGTNYKFSPEAFAALG
                     ATERFIGIKDTTCDLAKVAKKIAAAKGTIKIMQAHTPTLLDSCKLGATGGINTSSNVC
                     PSLFQKLFDLFDAGDLETAERLQQRIIQIDEMMSDGYVKSAKVAVSMMGVPMKHFTRN
                     PSKDFTPERMEELKKMCELIEESEKEFGIVHRSI"
     gene            complement(1226..3220)
                     /locus_tag="PLT86_08110"
     CDS             complement(1226..3220)
                     /locus_tag="PLT86_08110"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT36614.1"
                     /translation="MEQKDKDAHKRVEEWTARAFDAAANTPPFSFVYGGERVLLSENR
                     WEPELLTATTAGHAREFSCVFRDRQTGLVCTCEFNQFLDFPAVEWLLHFENAGTNATP
                     IISDILPLDTLFAGAGASTIRHANGSLCRSDDFAPHETALKPGETFRIGTTGGRSSNG
                     ALPFFSLHTDKERVIGGIGWTGNWRCTFAANETGGVRVSAGMQKTHLSLLPGEKIRSP
                     RILLLFWNGEPIDGQNALRRFLLAHHTPLAAGNPPEVPLSFVGWGENRSERQIAKARW
                     FALQEIPLDNYWIDAGWHGDAEFCDKATVFNTNWGAHVGNWWPNRSNYPEGMAPIGKV
                     TRELGMKFTLWFEPERVFKGTFLTRHHPEWLSGPIGDNWLFDLGNREARAGLVDIISS
                     VITEGGITVYRQDFNMDPEPFWNSMDTPERVGMAEIRHIEGLYAFWDELMDRHPGLLI
                     DNCSSGGRRIDLETISRSIPLWRSDYQCYPDFDPIGMQGQTHGLSLWVPLSAGCCDRP
                     DTYAFRSTLIPGATLTTTVARPDEPEGYRTPWDAFPVEWLQTMAKQYQELKPYALGDF
                     YPLISFSLADDVWAVWQFHRADLESGVILAFRRQNSPYPEMRVPLKGVLPNARYRIQS
                     YDGPGARIWRSGDLTAEGFSIIIGERPGSRLFRYELAEKA"
     gene            3407..3817
                     /locus_tag="PLT86_08115"
     CDS             3407..3817
                     /locus_tag="PLT86_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT36615.1"
                     /translation="MVTGIGHAAYGVSDLNASLRFYCGQLGLRKAFELHRDDGTLWIV
                     YLYAGNRSFIELFPESEVPPQEGGSYRHLCLIVDDMAATLRAMKIAGLEPLNPPSVGK
                     DGNTQAWVRDPDGNPIELMQIAPDSDQARAITSG"
     gene            3932..5410
                     /locus_tag="PLT86_08120"
     CDS             3932..5410
                     /locus_tag="PLT86_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT36616.1"
                     /translation="MVVRLAVAALIGLWNLSAAHAAERLRVVVHELDANGVSPQVARV
                     VSEQLRSKLIETRRFVVPEREKMEAVVAEQSVSLSLLACSSQECAIELGRILQANKMV
                     VGSVSLLNQTYSINTRFLDLETGAAEFSAEEKCQTADDLFLAAERLAARIAASVPQRG
                     TVTAVSGENIIIDLGALDGVSTGMQFRVLRRVERVQGYWEEEQVALAVVTSVQPTWSR
                     IEPQRAGLNFMRPVSIQQNDIVVGVQSITVDELPRYAFLSVYSRPVGAEVYVDNLFQG
                     RAPESGLEVRLTPGKHTVRVSAPAHRTEERSIELRPDERRSFNATLQPRLRRIPFRMQ
                     VLTGGYVQDRPANAAFRSRLENERMNGARVGIGRVYSVFMSEAGGSWTHANMPADRSF
                     GLNEVHNLSLHAQGGISPRLGWVLPYVALGYEFSRFYFNDQNVFEGQQSLGDAGKVGN
                     SGWYWGAGAFLGHWFHVGLRQTIGRPETDFRSLTIGVNLAGF"
     gene            5410..6585
                     /locus_tag="PLT86_08125"
     CDS             5410..6585
                     /locus_tag="PLT86_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOT36617.1"
                     /translation="MGLYRWTSGVGAVVAMALVACGCSDDFRGTLLAPLMFSEAANDS
                     SGLYREPNDTPEQAILIRTDGTRTRQTIHLPSDVDYFRFLAAAGAQYLVNVETETGGS
                     LQLEITDERGVVQPVTVVNAPGANRNTRSWTTAESGYYFVRVSAPVAANSVVGTYSVW
                     IVVGEDGFEPDNTISQAKEIAINGTWQQHTLPGMADKDYMSFSAWANFHYEFLFDGAS
                     GEHVSFAVVDGSGNSVSSRGAPSLVRFDAPSDGTYFVEVIPVDLGALGTYRVSVFSTD
                     PWEPDSTLALATPISTTGEPQRHSFSRVSDQDYLKFQAAAGVTYIIRTDSLGYGVDTQ
                     IEIDSASGTALVSDDNSGGGNASMLVWTAPVTGAFFVRVRSTTGGFPGKYVVSIKSN"
     gene            6691..7326
                     /locus_tag="PLT86_08130"
     CDS             6691..7326
                     /locus_tag="PLT86_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36618.1"
                     /translation="MRRSIVFPLVAAGAMFVLSACGSKPPEVGQQFIIERVPDERPRW
                     ITRPPADRDDKLFFVGLKTHASSLENGNTDARQNAIQKIVEYVGGTGMVDYTKARVES
                     GLTDEGQAGNYVEDGFRFLAESIAQGMREDENYYERVKEWQPTGWRYFYNYHVLVSIP
                     KNSLADAARQAFERQAAAARARNDARAEAFANKLREQLTPGMTPQSTVGGQ"
     gene            7412..8950
                     /locus_tag="PLT86_08135"
     CDS             7412..8950
                     /locus_tag="PLT86_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36619.1"
                     /translation="MKRHLPFPCVRLLVGVSAVLLVASCGPKTVNTMPRGERRLVEAS
                     ADRVPDWITREPASDAEYHYFRGIRTDAPSLEAGETDARQNALASIVQFLGLRVTVDY
                     QRLRTEEQTRIADAIRSVGGADLFGTRLSEMFYRRWRVIDGGEVHDKYDVYLIVRFPK
                     ESVDRIKRNQQERLQAISRLVGNPAGEPSDPSGFYAQISSISQALRALTDLNQSVLIT
                     TETEGQAQQLKALATTKLLRLVGSARLSLTPPAARIATGRHNPPLVFSCLATNDRGVP
                     IPNLPLRLSVGDSSQVVWSNASGVAEVIRSEIHSTNGRFLFSAQLDLPVDVRSLPDIA
                     RTVPSVSSSLEVVPRTETIRLLVVVDEWHNRRSPSRRTAEERLIAALRERGFTVVSAA
                     EFPRNEIGNPWSNQSDAFDLARKSHSDFLVWGSVETGEATLVPYSTSLYATSATAQIR
                     AIDAVSQNVMATVVLPDGVMRDTRGFGNTPERAAEEAVTLARRDFPNGYAYLAEQLER
                     ALTQ"
     gene            complement(9013..9714)
                     /locus_tag="PLT86_08140"
     CDS             complement(9013..9714)
                     /locus_tag="PLT86_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT36620.1"
                     /translation="MRLLSSLFRWFCGWIVFVVGAPWILLCGLAATPERFFRVLQVWS
                     RALLRVLSIRVVLHGEFPARETGAFVAVAPHVNLFDPFVMASILPRPIAGVELDKHFR
                     WPLYGRIIARVGNIPLSHTTPQRTRESLRLVEERLKKGQPLVVFPEGHRTRTGRRGEF
                     GSWIFRVAARTGTPVVPIAFRGAWERNHVGSLLITPGTWEVEILPPIIPAGGDRAAAE
                     ALKKAVEEAIDRKTQ"
     gene            complement(9756..10670)
                     /locus_tag="PLT86_08145"
     CDS             complement(9756..10670)
                     /locus_tag="PLT86_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOT36621.1"
                     /translation="MADEEKRWLAIVNPTAAGGKCGERIGDAVDELRWRGLAVTTRFT
                     TKPGDASTLAAEGIAEGYTRIMAVGGDGTVNEVVNGIAKTRADGTITLATLPLGTSNS
                     FLRDFNQQKLPNAIARITAGTAPPCDLVRCRILVDGVPTERWFINNVIVGFGANVGDT
                     MNRRLKFMGKHGYSVGVFIEVARLKTPAMRITVDGVVRSGPVTMVNIGNSQFTGGVMH
                     ISPGALVDDGLFDVVTIGKLSRTGLLLAFPRIFNGTILSHPKITHARGKRLTLETDNP
                     LPVLFDGDISGTTPLEAELVPGAILVIR"
BASE COUNT         2315 a         3207 c         3364 g         2296 t
ORIGIN      
        1 cttggaatcg agatctggga cgaatcgcgt tttctggcgg aactggggga atgacgcccc
       61 ttgttcgaac aactgtgggg cccttggttg acccattctt gaatgcgctt catctttcat
      121 acatggaaat cacctgacgg ccgtgcggca ccggcgggtg gatgcatttc cctgaacagt
      181 attctggttc cttgcccctg ccgcgggagg atttcgcagt gaagaatttc aaaccggcgt
      241 acgggattgt tccgcctctt attacgccgt tcaacgagga cttgtcgatt gactggtacg
      301 cctacgacag actgattgac tggcacattg agcggaatgt caccggtctt tttgtcgtgt
      361 gcgggtcgag cgaatactgg aagctgaccg aggacgaagc cattcagatg gcgaaggccg
      421 cggtgaaacg agcgaacgga aggattcata tcctggccgg gagtaccaac tacccggacg
      481 acgacctcac caagaacatc aacatgacga agcggatggc agacacgggc gtggacggct
      541 gtttcattac tacaccccgc acgatcccgc cccaggacga cttgatgttg gattatcaca
      601 tgaagatcca cgatgccgtc aattatccgc tgtacgcgta tgagatgccg ggcgggacca
      661 attacaagtt ctctccggaa gcgttcgcgg cgctcggagc gaccgaaaga ttcataggga
      721 tcaaggacac cacgtgcgac ctcgcgaaag ttgcgaagaa gattgccgcg gcgaagggga
      781 cgatcaagat catgcaggca cacaccccga cgttgctgga ttcctgcaaa ctcggcgcga
      841 ccgggggcat caacacctca tcaaatgtgt gccccagtct tttccagaag ctttttgatc
      901 tgttcgatgc gggggacctc gaaacggcag aacgcctcca gcagcggatc atccagatag
      961 acgaaatgat gtccgatggg tacgtgaaat cggccaaggt cgcggtcagt atgatgggcg
     1021 ttcccatgaa gcatttcacc cgcaatcctt ccaaggactt cacaccggag cgcatggaag
     1081 agctgaaaaa gatgtgcgaa ctgatcgagg aaagtgagaa ggagtttggg atcgttcacc
     1141 gatcgatctg aaaacgcgtt cgatcgggac caatcagaag cgggagactc gttgaacgag
     1201 tctcccgctt ctttttttgc agtcatcacg ccttttcggc gagctcgtaa cgaaacaggc
     1261 gggagccagg tctttcgccg ataatgatgg agaatccctc ggcggtgaga tcgccgcttc
     1321 tccagattcg tgcgcccggc ccgtcgtacg actggatacg ataccgggcg ttcgggagga
     1381 ctcctttcag gggaacacgc atttccggat acgggctgtt ctggcgacgg aatgcaagga
     1441 tgacgcctga ttcgaggtcc gcacgatgga actgccatac cgcccacaca tcgtccgcga
     1501 ggctgaaaga gatgagcggg taaaaatctc cgagcgcgta gggcttcagc tcctgatact
     1561 gtttcgccat ggtttgaagc cactctacgg gaaacgcatc ccagggtgtc cgataccctt
     1621 caggttcatc ggggcgggcg acggtagtag tgagtgtggc ccccggaatg agcgtgctcc
     1681 ggaatgcata cgtgtcaggc ctgtcgcaac accccgcact caggggaacc cacagagaaa
     1741 ggccgtgtgt ctgcccctgc atcccgatcg gatcaaaatc gggatagcac tgataatcgc
     1801 ttctccagag cgggatactc cgggagattg tttcgaggtc gatgcgtctc ccgccgctgg
     1861 agcaattgtc gatcagcaac cctggatggc ggtccatgag ctcgtcccag aatgcgtaca
     1921 agccctcaat atgccggatt tcagccattc cgactcgttc aggcgtatcc atggaattcc
     1981 agaacggttc gggatccatg ttgaaatcct gccggtagac ggttatcccg ccttcggtaa
     2041 tcacggatga gatgatgtcg accagtcctg cgcgcgcttc gcggtttccc agatcaaaca
     2101 gccagttgtc tccaatgggt ccggaaagcc actcagggtg gtgccgggta agaaaggtcc
     2161 ccttgaacac gcgctccggc tcaaaccaaa gtgtgaattt cattccgagt tcgcgggtta
     2221 ccttcccgat cggtgccatc ccctcggggt aattgctcct gttcggccac cagtttccga
     2281 cgtgcgcgcc ccagttggtg ttgaacacgg tggccttgtc gcagaattct gcatcgccgt
     2341 gccatcccgc gtcgatccag tagttgtcga gagggatttc ctgtaacgca aaccagcgcg
     2401 cctttgcgat ttgccgctcg ctgcggtttt cgccccaccc cacaaacgag agaggcactt
     2461 ccggcgggtt tccagccgcg agcggcgtat ggtgggcgag aaggaagcgt ctgagagcat
     2521 tctgcccgtc aatgggttct ccattccaga agagcaacaa aatgcggggt gatcgaatct
     2581 tctcgccggg cagaagcgac agatgggtct tctgcattcc cgccgacacg cgcactccgc
     2641 cggtttcgtt cgcggcgaac gtgcaccgcc agtttcctgt ccacccgatg ccgccgatca
     2701 cccgctcctt gtctgtgtgc aggctgaaga acggcaaagc cccgttggag gaacgccctc
     2761 ccgtcgtgcc aatgcggaat gtctctccgg gtttcagagc cgtttcgtgc ggggcgaaat
     2821 cgtcactgcg gcagaggctt ccattggcat ggcgaatcgt tgatgctccc gcgccggcaa
     2881 acagagtgtc caggggaagt atgtcgctga tgattggagt ggcgttggtg cctgcgtttt
     2941 caaagtggag gagccattcg accgcaggga aatcgaggaa ctggttgaat tcgcacgtgc
     3001 acaccagtcc tgtttgacgg tctcggaaaa cgcatgagaa ttcccgagca tgcccggcag
     3061 tagttgcggt cagcagctcg ggttcccagc ggttttccga aagcagaacg cgttcgccgc
     3121 cgtacacaaa agaaaacgga ggggtgttcg cagcggcgtc aaaggcccgt gcggtccatt
     3181 cttcaacgcg tttatgagcg tctttgtctt tctgttccat ggtcttcgtc cttcggaaat
     3241 caacgtgccg ttccgtcgcc cggtgaagag aatgttactc tacccggcgg ttcgaaccca
     3301 cgccctcccc cgttccgttt ttggcgtcca gggggtaggt tcaatggggc tttgtcgcga
     3361 ttcatttttt ccttcgcgaa cttatccacg gaaaggagtc aactcaatgg ttaccggaat
     3421 cggtcacgct gcatacgggg tcagcgacct gaacgcgagt ctgcgttttt actgcgggca
     3481 actcggtctg aggaaggcct tcgaactgca tcgtgatgat ggcactctgt ggattgtata
     3541 cctctacgcc ggtaatcggt cgttcatcga acttttcccg gaatcggagg tgcctccgca
     3601 ggaagggggg agctaccgac acctttgttt gatcgtagac gacatggcgg caacactgcg
     3661 tgcgatgaaa atcgccggtc ttgagcctct gaacccgcca tccgtgggaa aggatgggaa
     3721 tacgcaggca tgggtgcgcg atcccgacgg caaccccatc gagctcatgc agatcgcacc
     3781 tgattcggac caggcccgtg cgataacgtc ggggtaatgt gagctctttt ggcacggcat
     3841 ctgcaggggg ctgtcatcag tcggaactcg gcgtgcgccc gatgacgagt tcatcgggac
     3901 acagaacgaa acctggagat caggattggt gatggttgtc cgtttggccg tggcggccct
     3961 cattggcctg tggaaccttt ccgccgcgca cgcggctgag cgactgcgcg tcgtggtcca
     4021 tgaactggac gcgaacggcg tatctccgca ggtagcgcgc gtcgtatccg aacagcttcg
     4081 atccaaactg attgagacac gacggtttgt tgttcctgag cgcgaaaaga tggaggctgt
     4141 cgttgccgag caatcagtct ctctgagcct cctcgcctgt tccagccaag agtgcgcgat
     4201 tgagctgggg cgcatccttc aggcgaacaa aatggttgtc gggtcggtct ccctccttaa
     4261 ccagacgtac agcatcaaca cgcgattcct agacctggaa acgggtgcgg cggaattttc
     4321 cgccgaggaa aaatgccaga ccgcggacga tctgtttctg gcagcggaac ggctggccgc
     4381 gcgaattgcc gcttccgttc cgcaacgagg taccgttacg gcagtgagcg gggagaatat
     4441 cattattgat ttgggcgccc tcgacggagt atcaacaggg atgcaattcc gcgtcctgcg
     4501 gagggtggag cgggtacagg gttattggga agaggaacag gtggccctgg ccgtggtaac
     4561 atccgttcaa cccacgtggt cgcggataga gccgcagcgc gccggattga atttcatgcg
     4621 tccggtttcc atccagcaga acgacatcgt tgtcggcgtt caatcgatca ctgtcgacga
     4681 attgccgcga tacgcgttcc tgtcggtgta ctcacgcccg gtgggggccg aggtgtatgt
     4741 ggacaatctt ttccaggggc gtgcgccgga atcgggactc gaagttcggc tgacgccggg
     4801 gaaacacacc gttcgtgtga gcgcaccggc ccacaggacc gaggaacgct ccatagaact
     4861 gcgccccgac gagcgcagat ccttcaacgc aacgctccag ccgcgtctga ggcgcatccc
     4921 gttcagaatg caggtcctca ccggcgggta cgtgcaggac cgaccggcga acgccgcgtt
     4981 cagaagcaga ctggaaaacg agcgtatgaa cggcgcacgg gtgggtatcg gcagggtata
     5041 ctcggtattc atgagtgagg ccggcggaag ctggacccac gcgaatatgc ctgctgaccg
     5101 cagcttcggt ctcaacgaag tccacaacct ttcgctgcat gctcagggcg gaatctcgcc
     5161 ccggctgggc tgggtcctcc cgtatgtcgc gttagggtat gagttttccc ggttctattt
     5221 caacgaccag aacgtgttcg aggggcaaca aagtcttggt gatgccggaa aggtgggaaa
     5281 cagcggctgg tactggggcg caggagcatt cctgggacac tggttccatg tggggctccg
     5341 ccagaccatt ggccggcccg aaacagattt ccggtcgttg accattggcg ttaacctggc
     5401 gggtttctga tggggctgta ccgctggaca tcgggcgtcg gtgctgtcgt cgcgatggct
     5461 cttgtcgcat gtggctgctc tgacgacttc cggggcactc tgcttgcgcc actgatgttt
     5521 tccgaggcgg ccaacgactc ctcgggcctg tatcgcgaac caaacgacac cccggaacag
     5581 gcgatactga tcaggacgga cggaaccaga actcggcaga caatccatct tcccagtgac
     5641 gtggactact tccggttcct cgccgctgcc ggggctcagt acctggtgaa cgtggagacg
     5701 gaaaccgggg gctcacttca gttggagatc acggacgaac gcggagttgt gcaaccggtg
     5761 acagtggtga atgcacccgg cgcgaacagg aacaccagaa gctggacaac tgcggagtca
     5821 gggtactatt tcgtacgggt gagcgcgccc gtcgccgcga actcggttgt cggaacgtac
     5881 agcgtttgga tagtcgtcgg ggaggacggt tttgagccgg acaacacgat ctcgcaggcg
     5941 aaggaaatcg cgataaacgg cacctggcag caacacaccc tcccggggat ggcggacaag
     6001 gactacatga gcttctcggc ctgggcgaac tttcattacg aattcctgtt cgacggggct
     6061 tccggcgagc atgtgtcgtt cgctgtggtg gatggttcgg gaaattcggt ctcgtcgcgt
     6121 ggtgcgcctt ctctggttcg gtttgatgca ccgtcggacg ggacgtattt cgtggaagtc
     6181 attccggtag atctcggtgc cctgggtaca taccgggttt ccgtcttctc cacggatccg
     6241 tgggaaccgg acagcacgct cgcccttgcg acaccgatct cgactacagg ggaaccgcaa
     6301 cggcatagct tttcccgtgt atccgatcag gattacctga aatttcaagc cgccgccgga
     6361 gttacctaca ttatcagaac ggatagtctc ggttacggtg tcgacaccca gattgaaata
     6421 gacagcgcat ccggcacggc gttagtttct gacgacaaca gcgggggagg aaacgcgtcg
     6481 atgctggtgt ggacggcacc cgttaccggg gcttttttcg ttcgcgttcg gagcaccacc
     6541 gggggattcc ctggcaaata cgtggtgtcg ataaagtcga actgatatgg cacgcacgtt
     6601 gcaatctggt taacgtgaat gttctgtcaa aaagcggttt tcatccagag catgtccgag
     6661 taccacaata ccgaaccagg aggttccgcc atgcggcgat caatcgtgtt tcctctcgtt
     6721 gcagccggcg cgatgttcgt gctgagcgcg tgtggctcga aaccaccgga ggtcgggcag
     6781 cagtttatca tcgaacgcgt tcccgatgaa cgtccgcgct ggataacgcg gcctccggcg
     6841 gatcgtgatg acaagctctt cttcgtcggc ctcaaaaccc acgcatcaag cctggaaaac
     6901 ggcaacaccg acgcccggca gaacgccatt cagaaaatcg tggaatacgt gggtggtacc
     6961 gggatggtgg actacaccaa ggctcgcgtt gagtccgggc ttaccgacga agggcaagcc
     7021 ggcaactacg tggaagacgg cttccgcttc cttgccgaat ccattgccca aggcatgcgc
     7081 gaagacgaga attactacga acgagtgaag gaatggcagc cgaccggctg gcggtacttc
     7141 tacaactatc acgtgctcgt cagcattcca aagaactcgc tggccgacgc cgcgcgccag
     7201 gcgtttgaac ggcaggcggc ggcagcgcgc gctcggaatg acgctcgcgc ggaagcgttt
     7261 gccaacaagc tccgggaaca actcactccc gggatgaccc cgcagtccac ggtcggtggt
     7321 cagtaaattc agctcaaacg gcgtgcgaaa gcatacgggg aagccgggtt caaccggttt
     7381 cccccttttc caaggctggc gattatttgc cgtgaaacgc cacttgccct ttccctgtgt
     7441 ccgacttctc gtcggtgtga gcgcggttct tctcgtcgcc tcgtgcggcc ccaaaaccgt
     7501 gaacacgatg ccgcggggag agcgccgcct tgttgaagca tcggcggacc gagtccccga
     7561 ctggattacg cgggaaccgg ccagcgacgc cgaataccat tatttccggg gcatccggac
     7621 agacgctccc agtctcgaag caggtgaaac ggatgcccgc cagaacgcgc tggccagcat
     7681 cgttcaattc ctcggcctcc gggtgaccgt tgattaccag cggttgcgaa ctgaagagca
     7741 gacgcgcatt gcagacgcga ttcggtccgt tggcggtgcg gatctgttcg ggacgcggct
     7801 ctcggaaatg ttctaccggc gctggcgggt tattgacggc ggggaagtgc acgacaagta
     7861 cgacgtgtat ctgattgtgc gtttcccgaa agaatctgtc gaccgaatca aaagaaacca
     7921 acaggagcga cttcaggcga tttcgcgtct cgtgggaaac cctgcaggtg aaccgtctga
     7981 cccgtcaggt ttctacgcgc agatttcgtc gatttcgcag gctctccgcg cactgacgga
     8041 cctcaatcag tcggtgctca ttacgacgga aacggaggga caggcccagc agctcaaagc
     8101 gctggcgacg acgaaactcc tccggctcgt cggctccgcg aggctttctc tgacaccgcc
     8161 cgccgcacgc atcgcaacgg gcaggcataa cccgcctctg gtgttctcgt gtctggcaac
     8221 aaacgaccgg ggcgtgccga taccaaacct tcccctcagg ctctcggtcg gagattcgtc
     8281 tcaggtcgta tggtccaacg cgtcgggcgt cgccgaagtc atccgttcag agatccattc
     8341 tacgaatgga cggttccttt tctcggcaca actggacctt ccggttgacg ttcgatcgct
     8401 tcccgacatc gcgcgcactg ttccttcggt atcgtcttcc ctcgaggtag ttccgcgtac
     8461 cgaaacgata aggttgctcg ttgtggtgga cgagtggcac aatcgccggt caccgtcccg
     8521 gcggacggcc gaggagaggc tcatcgccgc gttgcgggag cgcggcttca cggtggtcag
     8581 cgcggccgag ttcccgagaa acgaaatcgg gaacccctgg agcaatcaga gcgacgcgtt
     8641 tgaccttgcc cggaagagcc attctgattt tctcgtctgg gggtcggtgg aaacgggcga
     8701 ggcaacgctc gtgccctatt ccaccagcct gtacgcaaca tccgcgactg cgcagatcag
     8761 ggcgattgat gcggtgtcgc agaacgtcat ggctacggtg gttctgccgg atggagtgat
     8821 gcgggatacc agaggtttcg gcaatacgcc cgagcgagcg gcggaggagg ccgtcacact
     8881 tgcgcggcgc gattttccca acgggtacgc ctacctcgcg gaacaattgg aaagggcgct
     8941 gacccaatag attgcgtcgg aaattaccgg acgattcccc gtgtggttcc tctccctcca
     9001 tccgtgaatc cgtcactgcg tttttctgtc gatcgcctcc tcaacagcct ttttgagagc
     9061 ttccgcggct gctctgtcgc cacctgctgg aatgatgggc ggaaggatct ctacttccca
     9121 cgttccgggg gtgatgagaa gcgaaccgac atggttccgc tcccatgccc ctcgaaaggc
     9181 gatcggaacc accggagtcc ccgttcgcgc ggcaacgcga aaaatccagg atccgaattc
     9241 accacgccgg cccgtgcgcg ttcgatgacc ttccgggaag acgaccagtg gttggccctt
     9301 tttgagacgc tcttccacca gccggaggga ctcccgtgtt ctttgcgggg tcgtatgact
     9361 caaagggata ttgcccaccc gagcgatgat tctgccgtaa agaggccacc ggaaatgttt
     9421 gtcgagctcc acccccgcaa tcgggcgagg cagaatcgaa gccatgacga aagggtcgaa
     9481 aaggttcacg tggggcgcca ctgcgacgaa tgcgccggtc tcacgcgcag ggaattcccc
     9541 gtgcaggact acgcggatgc tgagcactcg caatagagcc ctgctccaca cctgcagcac
     9601 ccgaaagaag cgttcgggag tggctgcaag accgcagagc aaaatccacg gcgcacccac
     9661 gacgaagacg atccacccgc agaaccaacg aaaaagagag gaaaggagtc tcatgtctga
     9721 atcctggagc aattcagctc ccgttcccat cggattcagc ggataaccag aatcgccccg
     9781 ggaacaagct cggcttccag cggcgttgtg ccggagatgt ctccgtcgaa aagcacgggc
     9841 aggggattgt cagtctcgag agtaagcctc ttgcctcttg catgggtgat tttggggtgt
     9901 gaaaggatgg tgccattgaa tatccgtgga aacgccaaca ggaggccggt gcgcgagagt
     9961 ttcccgatgg tgacgacatc aaaaaggccg tcgtccacca gtgctccagg cgaaatatgc
    10021 atcacgccgc ccgtgaactg cgaattcccg atgttcacca tggtgacagg gcccgaacgc
    10081 acaactccgt ccacggttat cctcattgcc ggcgtcttca ggcgcgcgac ctcgatgaat
    10141 acccctacgg agtacccgtg tttgcccatg aatttcaggc ggcggttcat cgtatcgccc
    10201 acgttggccc caaaacccac aatcacattg ttgatgaacc atcgttctgt tggaacaccg
    10261 tccacaagga tgcgacaccg gacaagatca cacggaggcg cagtgcctgc ggtaattcgc
    10321 gcgattgcgt tcggaagctt ctgctgattg aaatcgcgga ggaaggagtt cgatgtaccc
    10381 aggggaagcg tcgcgagggt tatggttccg tccgcacgcg tcttcgcgat accgttgacg
    10441 acctcgttca cggtgccatc accgccgacc gccatgatac gcgtgtaccc ttcagcgatt
    10501 ccctcggctg ccagcgtgct cgcgtcgccg ggtttcgtcg tgaagcgtgt ggtcactgcc
    10561 aggccacgcc acctgagttc gtcaaccgcg tcccctatcc gttctccgca cttgcctccc
    10621 gccgcggtag ggttgacgat tgcgagccag cgtttttctt catccgccat atgcgcttcc
    10681 atgcatgggt acgaccgttg tcgccacggt ctgacgggtc tgcgcgacag acctggcact
    10741 caagtcccac gaacgcgtgg ctttcaaggt tgttcaagag tacaaaacgg agctagattt
    10801 accaaacccg cgccaaaggc agttgcttcg ggacagttgg cgagacgccg ttgcatttcg
    10861 aggaagactc gtgctcgcgt cagaacgtgc gaccggtagt gaattgacgc ttcgtatctt
    10921 ggtggcaacg ggcgcctgaa acggacgtgc ctgaagcgga cttccaactc gttgccggtc
    10981 tcgcggcaga caacgcgaat gcggtgggtc cggaatgcat gagacccact caggcgcggg
    11041 aagatcgccc ggccgccatt ccaaagacgg aggccacccg gctccgattc cacattcaga
    11101 acggcgacct gttcggctgc aaggcttgcg aagagtgacc cgtggaggac gacgcgttgc
    11161 ggaagcgagt gctgatggta at
//
LOCUS       DAOSOO010000096        11181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5803_length_11181_cov_5.059500, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000096 DAOSOO010000000
VERSION     DAOSOO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5803_length_11181_cov_5.059500"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3784)
                     /locus_tag="PLT86_08150"
     CDS             complement(<1..3784)
                     /locus_tag="PLT86_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36622.1"
                     /translation="MWQILATLVFGVGLPVCSTAQPAATTSSIGGAEVPSARQRGLKL
                     VLGNAGFNRLTVEQRENLLSGRTMCATSYLAKSEQPYRTARPSRVASYADLPTVGDHL
                     QNKKYRLVLIFIATAGHDSARPVLIRNSFLPGRVPATTSSTPNWMQNDTLSYDTYVKD
                     AIEYFFDKNSYGALDVDVVIPYPPNSSSPIWVSPDTAGYGVVDSVLKRIETSYPDSFF
                     YDVSPPSPYDYQRVGYLFTDTFATGWCSSDSNELWASEAIRDSIWDGATFAGAAWAGT
                     GNPLWPDVHSETFAWQIIHEMLHHITYANASHPSFADRGVREFGTRTWGFDIMDHGGK
                     ACATNGHYGAHALAPIDKYLLGFIEGNWLRTITTNTTDVRLSPNHVSPPSGGRLLARI
                     PLSSGTSGTESFVVSNHQGIGIDEVYNPGMGDSLNNGLHIWHIGDGFELGFQSNRADV
                     EVAWTIALGDTFCHPEGQSHNPSSWAVSQYLPTEYSDNFDWIDGVLYDSPLYPSMGGK
                     GLYILNQAYGGWSAGGPGIGNSTAFCNHNSDSGTLRWEHLYDRNIITPTINDFFRPGN
                     AFTPYSRPNTDKYHRYAVTSYADSMVCTQGEPQEDRPRDGNIMLPLGGAPFVSSQLRD
                     VNDGLTRVGIVNIRQETNGDMLFDVYFNFWEGEFNDARNFGYLKRYDDGTECESGSVC
                     WRNVWESMTLAGVAHDTVVVGPNGFLINTGRKITVKTGTTMKVLGPITVEGTLAVESG
                     VIFDTIKGDIVIASGGRLELGSNVTLPFDLDHCKDLHVDSAGTLIGSGPTSVIEGPIN
                     VRGRSATVQNVKVLFADPYGTGQSIVTVSNGWPTDTTTLIKDCILSGAPNTENHYGIG
                     CFGSSPRIRGVAVDTVYAGIYAYNGSDPVIYRSAVYDDPDCEGTVTNNLGASCYYGVQ
                     AYLNSEYQGGYYNPPNTRFGGNNFRRANSATGAHVYASNTGQQKLDYNYWNGGPDVLV
                     YNGAYATTYPQCGSPFPRPGRSGGDERQPVFAAIDSAVAVLADHDTTGALDNLRALAG
                     RQTEAMDALFVLDRITELCSPERVVSLTEGLRTPESRPAARQAAMWVMGRARHAQGEL
                     LGAAQWYAQVRELDADSLLATESKFAEAELLFDLAGQRDRALVLFREFLAEASVDNPN
                     RVIARGKLKMAGQDGDEPEPRITRPASPPLLTARPNPFNPTTSITYRVTAQGNVSVQV
                     YNALGQRVRELVNTAAPAGTYTVVWDGKDSAARPVGSGVYLIRLTTPAAVVTERVTL"
     gene            complement(3807..4895)
                     /locus_tag="PLT86_08155"
     CDS             complement(3807..4895)
                     /locus_tag="PLT86_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36623.1"
                     /translation="MKKSILCLAADGLAVLCLFAHGATAKTDSLTTGQRIQRVFPFQV
                     GQQYEFQSGVTFHRPTGQALPPYTIAQITITDTVIDGKTYLHIPYWSSFGTEYYRLDD
                     SLRIRNYNPDSSKEEVFLNLGYGGTLMFPGMGFECLGTCRMSGYSRSGSFTGIDKPFH
                     LAYWVYYDNPSYTKLTVAYQAWTTASRTTTETGITTDADSALAYGFDLCCESTSVTNI
                     FGVGGDGSVQHRQGVTDRQRRLAVFRPVSNEPWLPLANAMGDYADVRTENNRPSQFSV
                     SAYPNPFNTNTLVRYSVPTTGHVTLTIYTIEGRLVRTLVDAVIVAGTHEAQWDGRGND
                     GKEAGGGVYVVRLVTPTNAVVERISLIR"
     gene            complement(5254..6282)
                     /locus_tag="PLT86_08160"
     CDS             complement(5254..6282)
                     /locus_tag="PLT86_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT36624.1"
                     /translation="MKWIDFPNDAFVVNGLPWFGETKPHLWRLPERMKGAVRPPVWDL
                     ATNPSGGRIRFRTNTANLSLKANFTTATTYCNISIIGQMGYDIYSDGLYWRSFFPHNA
                     SAPQVGVVEEAVFQDHPREWRDVCIYTPIYAPGEVLAFAFDDDAEFLPPAPFAVEKPV
                     VFYGSSITQGGCASRPGMSYQAILGRALNIDFVNLGFSGNGMGEPELAEAMAEIDASC
                     YVVDFAQNVGDPVKLREVYGPFLSILRAERPDTPIVCNTPIYASKALWWPEGQRNLEG
                     QRQVIRDAVAERKAMGDTRVWLTEGTDFLGPSLSDAFVDGVHPSDLGFQAIADGFRPV
                     IARILGLA"
     gene            6428..8833
                     /locus_tag="PLT86_08165"
     CDS             6428..8833
                     /locus_tag="PLT86_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36625.1"
                     /translation="MSVGVERRGDTVVVRAGAYEVVISEAGVVIQHEATQGLTPIARS
                     VRIATAEDNEIPALAGDIVRIWYPDSRCVALEWVYQKGAHTVTTEMRVGCGDSLVSLH
                     ARDAINGEAEVRRFSAMWEFLAGPLNGGRPDFVWTPCLCPEPGQIVGEHVFRSPAAIV
                     QHGEVSFALVPDLSRISAHQPLRSFLDLHCSNSEQGLTCLSSGVCVQKVEGHVFFTTE
                     GVAPVVLRDTSIEFGCDIFVDALASRMRGHQAVSRMYWKNHGREWLRDIRPQVLPFRG
                     YYEYGYDFADKHLWEETRANEVRVGAMTISREYPHDIWFQGWFNQLRSAYGLFRWARE
                     TGNKERMGRALATRELLFQTPQKNGLFPAIAVLAPADGRPVEWVSSSLQGGGPGVYHL
                     LDSSWTAYWLLQWQLDCEPDVRTLPFCTAYADELVRLQRSDGSFPDYVDVDTHSCVTH
                     YDPTERAARSSSGYVRAMVSRWGTKRLPASAETAIHAMFLAELSRIVVDGEQYFRSAQ
                     DAARYVERNVLPFHKWWDFETFFSCSPKPLGFFDSRSQQYPQNTLSLFWASEAFRILY
                     EITGTAAIGEQSLALTDYLCLFQQVWSPPFLSFYGFGGFGVMNTDGEWNDARQALFAE
                     GLARQYLQSGRQEYLERAIAATRAGFACAYIPENADICPRIFARKPTGYADENYAHGG
                     YDAPAGPSGFDWGIGSALTTAIRMLDWFGDLWVDVYGGWALGIDALTVTSCRREGDVW
                     HLTVDAPLRHTDTITVKGNPGEGEPVCLSLNGQQPVVLTDEALRKGTTVRLYPPGQDP
                     CTR"
     gene            complement(8875..8948)
                     /locus_tag="PLT86_08170"
     tRNA            complement(8875..8948)
                     /locus_tag="PLT86_08170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8912..8914),aa:Met,seq:cat)
     gene            complement(9439..10659)
                     /locus_tag="PLT86_08175"
     CDS             complement(9439..10659)
                     /locus_tag="PLT86_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36626.1"
                     /translation="MRILLSCVGGRDPHAEADMSEGAIVSLTRHLLAIGQQPERVSLF
                     VTTGANSLETKADETAFWLSDSVGIAAEAISICRLTECNPAIADEVFDAMRAALTAET
                     ASFPADAEIHVGVSSGTPQMAQAWSVLAMLGLLGNATLWQVMDPKQPVADGKRVRQIS
                     LHGFRRPSILDEVQRAADLFQFSRAARSLRLFCSSAPQTQSVEAMRALARVLEGWDAC
                     DRWDFAGGKERLEGALKSLNSHPACEPVAQLVKRQLDPFPHNNPRNARMFTFFSAERR
                     IARNDPIAALLLAVETLENALDDYLRRSDLLLKDLLIVAHEGSDKRWLAVEDAAGGWD
                     ELGGLRHARNDVAHDMKPPKGEMVAKARVVARTALIALCGHSEAEIDGHPFSPGLQKN
                     AVLNTIDRLRREPA"
BASE COUNT         2273 a         3245 c         3301 g         2362 t
ORIGIN      
        1 acagcgtgac cctttcggtc acaaccgccg ccggtgtcgt caggcggatg agatatactc
       61 cgctcccaac cggccttgcc gcgctgtcct taccgtccca cacgacggtg tacgttcccg
      121 ccggcgcggc ggtgttgacc agttcgcgca cgcgttggcc aagcgcgttg tacacctgca
      181 ccgacacgtt tccttgcgcc gtcacccggt acgtgataga tgtcgtcggg ttgaacgggt
      241 tgggacgggc agtcaagagc ggaggactcg cgggcctcgt gatccggggc tccggttcat
      301 ccccgtcttg acccgccatt ttcagtttcc cccgcgcgat cacacggttg gggttgtcca
      361 ccgatgcttc ggcgagaaac tcgcggaaca gaacaagagc ccgatcacgc tggcctgcaa
      421 gatcgaagag cagctccgcc tccgcgaact tggattccgt cgccaacaac gaatcggcgt
      481 caagttccct cacctgcgcg taccactgcg cggctccaag tagttcgcct tgcgcgtggc
      541 gagcccgccc cataacccac atcgccgcct gtcgcgccgc ggggcgcgat tccggcgtgc
      601 gcagaccttc cgtcagtgaa accacacgct ccggagagca gagttcagtg attcgatcca
      661 gcacgaatag ggcgtccatc gcctcggttt gcctgcccgc cagggcacgc aggttgtcca
      721 aggccccggt cgtatcgtgg tcggcaagca ccgccacagc ggagtctatt gctgcaaata
      781 ccggctgtcg ttcatcccct cccgagcgcc ctggacgggg aaacggggaa ccgcactgag
      841 ggtatgttgt ggcgtacgcc ccattgtaca cgagcacatc ggggccacca ttccagtaat
      901 tgtaatccag tttttgttgc cccgtgttac tggcatacac gtgggcgccg gttgcggagt
      961 tcgcacgcct gaagttgttt cctccgaatc gtgtattggg aggattgtaa taaccgcctt
     1021 ggtactctga attgagatag gcttgcaccc catagtaaca ggaagcgcca aggttgttgg
     1081 tcacggttcc ctcgcagtcg ggatcgtcgt agacagcgga ccggtagatc acgggatcgg
     1141 aaccgttgta cgcgtagata ccagcgtaaa ccgtatctac ggccacgcca cggattcgtg
     1201 ggcttgaacc gaaacacccg atgccgtagt ggttttccgt gttcggcgcg ccgctcagta
     1261 tacagtcttt gataagggta gtggtgtcgg taggccaacc gttactcacg gtgactatgc
     1321 tttgcccggt accgtagggg tcagcgaaaa gaaccttgac gttctgcacc gtggccgaac
     1381 gaccgcggac attgatcggg ccttcgatga cgcttgtggg acctgaccca atcagtgtgc
     1441 ctgctgaatc cacatgaagg tctttgcagt gatccaaatc aaatgggagg gtgacattcg
     1501 atcccaactc aagccgcccg ccgctggcga tgacaatgtc gcctttgatt gtgtcaaaaa
     1561 taacacctga ttcaaccgcc aacgtacctt cgaccgtgat gggcccaagt accttcatag
     1621 tcgttcccgt tttcacggtg atcttccgac cggtattgat caagaaccca ttcgggccta
     1681 cgacaacggt gtcgtgcgcg acacctgcca aggtcatgct ctcccagaca ttgcgccaac
     1741 acaccgagcc gctttcacat tccgtgccgt cgtcgtaccg cttcaggtag ccgaaatttc
     1801 tggcatcgtt gaactcgcct tcccagaagt tgaagtacac atcgaacaac atgtccccat
     1861 tcgtttcctg gcggatattg acgataccaa cccttgtcaa accgtcgttg acgtcccgca
     1921 gttgactgga aacaaacgga gcgccgccta acgggagcat gatatttccg tctctcgggc
     1981 ggtcctcctg cggttcgcct tgcgtgcata ccatggagtc cgcatacgat gtcacggcgt
     2041 atcggtggta tttgtccgtg ttcgggcgcg aatacggcgt gaacgcatta ccggggcgga
     2101 agaaatcgtt gattgtcggt gtgatgatgt tccggtcgta gagatgctcc caacgcagcg
     2161 tgccgctgtc ggagttgtgg ttgcaaaacg ccgtgctgtt ccctattccc ggccctcccg
     2221 cgctccatcc gccgtacgcc tgattgagga tatacaaccc ttttccgccc atggaagggt
     2281 aaagggggga atcatataac acgccatcta tccaatcaaa attatcggag tactcagtgg
     2341 gtaagtactg agagaccgcc cacgaggagg ggttatgtga ttgaccttca ggatgacaaa
     2401 acgtgtcgcc gagggcgatc gtccaggcaa cttccacatc agctcggttg ctttgaaagc
     2461 cgagttcaaa gccgtccccg atatgccaaa tgtgtaatcc attgttgaga gaatctccca
     2521 tccctgggtt gtacacttcg tctatgccaa tcccctgatg gtttgaaacc acgaaagatt
     2581 ccgtgccgga cgtgccagag ctgagcggga tacgcgccaa aagtctgccc cccgacggag
     2641 gactgacgtg gttggggctc aggcgaacgt cggttgtgtt agttgttatc gtcctgagcc
     2701 aattcccctc gatgaaccca agaaggtatt tgtctatcgg agcgagcgca tgtgccccgt
     2761 agtgcccgtt cgtcgcgcac gctttcccgc catggtccat gatgtcgaat ccccacgttc
     2821 gcgttccgaa ttcccggacc cctctatcgg cgaacgaagg atgtgaggcg ttcgcatagg
     2881 tgatatgatg gagcatttca tggatgatct gccaggcgaa ggtttcagaa tgcacatcgg
     2941 gccacaacgg attacctgtt cccgcccacg cggccccagc gaatgttgcg ccatcccaaa
     3001 tggaatcgcg aatcgcttcg cttgcccaga gttcgttact atccgaagaa caccaccctg
     3061 tagcgaaagt atcggtgaat aggtagccaa cccgttggta atcatacggc gacggggggg
     3121 atacatcgta gaaaaatgaa tctggatacg aagtttctat gcgtttgaga acgctgtcga
     3181 ctacgccata gcctgcggta tcaggcgata cccagatggg cgaagatgag tttggtggat
     3241 acggtatcac aacatcaaca tcgagagcac cataggagtt cttgtcgaag aagtactcga
     3301 tcgcatcttt gacatatgtg tcataactaa gtgtgtcgtt ttgcatccag ttcggggttg
     3361 aagatgtcgt tgctggaacg cgccccggaa gaaaactgtt gcggatcaaa acaggccgtg
     3421 cggaatcgtg tccagccgta gcaatgaaga tcagaaccaa gcggtatttt ttgttctgta
     3481 gatgatcgcc aacggtagga aggtcagcat agctggcaac gcgagaaggc cgcgctgtgc
     3541 gatagggttg ttcacttttc gcgaggtaac tcgtggcgca catcgtgcgc ccgctgagaa
     3601 ggttttcgcg ttgttcgacc gtgaggcggt tgaaccctgc gttaccgagc accagtttca
     3661 ggccacgctg gcgtgcggag ggtacttcag cccctcctat cgaagaggtc gtcgctgcgg
     3721 gctgcgcggt ggagcagacc ggaagaccaa caccgaacac gagagttgca aggatttgcc
     3781 acatgacact ctcccccttt ttctgttcat ctgatcaggg aaatcctttc gacaacggca
     3841 ttcgttggcg tcaccaaacg aacaacatag actccgcctc ctgcttcttt tccgtcattt
     3901 ccacggccgt cccattgggc ttcatgcgtt cccgcaacga tcacagcgtc cacaagcgtc
     3961 ctcaccaaac gaccttcgat cgtgtagatg gttagtgtta cgtggcctgt cgtcggcacg
     4021 gagtagcgaa caagcgtgtt tgtgttgaac gggttgggat aggcggatac actgaactgg
     4081 gaaggccgat tgttctcggt gcggacgtct gcataatcgc ccattgcgtt cgctaacgga
     4141 agccatggct cattgctcac gggtcggaat accgccaacc ggcgctgtcg gtcagtgact
     4201 ccctgccggt gttgaaccga accatcgccg ccgacaccga aaatgttcgt aaccgaagtg
     4261 ctctcgcagc agagatcgaa cccgtacgcc agggcggagt ccgcatccgt tgtgattccc
     4321 gtctccgtag ttgttcgtga cgctgttgtc catgcctggt aggcgacggt gagcttggta
     4381 taactggggt tatcgtagta aacccaatag gcaagatgaa aaggtttgtc tatccccgtg
     4441 aagcttccgc tccgcgagta gccgctcatt cggcacgtcc cgaggcactc aaaccccatg
     4501 cccggaaaca tgagtgttcc cccgtacccg aggttgagaa atacctcttc cttgctgctg
     4561 tcggggttgt agttccttat ccgcagtgag tcatcgagcc gataatactc agtgccaaag
     4621 ctcgaccaat aggggatgtg gagataggtc ttgccgtcaa taaccgtgtc cgtgatcgtg
     4681 atctgggcta tggtgtaggg aggaagggcc tggccggtgg gacgatggaa cgtgacacca
     4741 gactggaact cgtattgttg gccaacctgg aacggaaaca cgcgttgaat ccgctgtccc
     4801 gttgtgaggc tatccgtttt tgcggttgca ccgtgcgcaa acagacaaag aactgcaagc
     4861 ccgtccgccg cgagacacag gatactcttt ttcatggttg cttctcctct gaagtcaaaa
     4921 ccccgcgcgg tcgccaccac gaacaacgta caaaaacccc ttcaggggcg cgaatctatt
     4981 tcaaggttgt ttatggaggt cgtgtaggct ggcagcgtgc tcgtgggcgt ttcaggcgtg
     5041 ttcgttggcg cggcccgtgc gcgccgctcg cggtaccccc ccccccggag gtggaagaga
     5101 tgaaacgaag aaaggcatgg ttcgcctcct ctcttcagcg ggtgccatgc aaggtgcggt
     5161 gctacctcct attatacacc aactcagtac gaaaagaaag gttccctgtc aagccccgga
     5221 caggcaggaa tgcctgtccc acccgggaag ccctcacgcc aggcccagaa tcctcgcaat
     5281 caccggtcgg aacccgtccg ctattgcctg aaagcccaga tcgctcgggt gcacgccgtc
     5341 cacgaacgca tcggagagcg atggaccgag gaaatccgtg ccttccgtga gccagacacg
     5401 cgtatcgccc attgctttcc tctcggcgac ggcgtcccgg ataacctgcc gctggccttc
     5461 gaggttgcgc tgcccctcgg gccaccagag ggctttcgat gcgtagatcg gcgtgttgca
     5521 cacgatgggc gtatccggcc tttccgcccg gaggatgctc aggaaagggc cgtacacttc
     5581 gcgcagcttc acagggtcgc ctacgttctg cgcaaagtcc accacataac acgaggcgtc
     5641 aatctcggcc atcgcctcgg ccagttccgg ctcgcccatc ccgttcccgc taaagccgag
     5701 gttcacgaaa tcaatgttca gcgcgcggcc caggatcgcc tgatagctca tgccgggccg
     5761 acttgcgcag ccgccctgcg tgatggatga accgtaaaac accaccggct tctccaccgc
     5821 gaacggggcg ggcggcagga attccgcgtc gtcgtcgaag gcaaacgcga gaacctcgcc
     5881 gggtgcgtaa atcggcgtgt agatgcagac gtcgcgccac tcgcgcgggt gatcctggaa
     5941 aaccgcctcc tccaccacac cgacctgcgg agcggaggca ttgtgaggga agaaggagcg
     6001 ccagtaaagg ccgtccgagt agatgtcata ccccatctgc ccgatgatcg agatgttgca
     6061 gtacgtggtg gcggtggtga aattcgcctt gagggaaaga tttgcggtgt ttgtgcggaa
     6121 acggatgcgt ccgccactcg ggtttgtggc aagatcccag acgggtggcc tgaccgcacc
     6181 cttcatgcgt tccggcagcc gccacaggtg gggcttcgtc tcgccaaacc agggcagccc
     6241 gttgaccacg aaggcatcat tcgggaaatc gatccatttc atgagaacct ccccacgtgg
     6301 caaaatggtt gaacaattca acaaatgaaa ccatcggggg gtgtgctgac gcaaccgggt
     6361 ttctgttccc gggtgtccgc ggtacttttc tggtggacag gaacgatccg gacggaagga
     6421 gtggggcatg tcggtaggcg tggaacggag aggcgacacc gtcgtcgtga gagcgggggc
     6481 atatgaggta gtcatttccg aggccggtgt ggttatccag cacgaagcca cgcaggggct
     6541 cactccgatc gcccgttcgg tacgcattgc cacagcggag gacaatgaga ttccagcgct
     6601 ggcaggcgac attgtgcgca tctggtatcc cgattcccgg tgcgtcgctc ttgaatgggt
     6661 gtaccaaaag ggggctcaca ctgtcactac cgagatgcgc gtcgggtgcg gagattcact
     6721 tgtaagtctt cacgcccggg atgcgataaa cggtgaagcg gaagtacgac gcttcagtgc
     6781 catgtgggag tttctggcgg ggccactcaa cggcggccga ccggatttcg tctggacgcc
     6841 ctgtctctgc cctgaaccgg ggcagattgt tggagaacac gtgttcagaa gcccggcggc
     6901 gatcgtacag cacggggagg tgtcgttcgc gttagtgccc gacctttcac ggatctccgc
     6961 gcaccaaccg ttgcggtcat ttctcgacct tcattgctcg aatagcgagc aggggctgac
     7021 gtgtttgtcc agcggcgtgt gtgtacagaa ggtcgaaggg catgtctttt tcacgacaga
     7081 aggcgtcgct cccgtggtgt tgcgggatac aagcatcgag tttggttgcg acatctttgt
     7141 ggatgctctg gcttccagaa tgcgcgggca tcaggccgtg agccgcatgt actggaagaa
     7201 ccacggacgg gaatggttgc gcgacatccg tccccaggtg ctccctttcc gcggctacta
     7261 tgagtatggg tacgactttg ccgacaagca cctgtgggag gaaacgaggg cgaacgaggt
     7321 ccgcgtcggc gccatgacga taagccggga atacccgcac gatatctggt ttcagggatg
     7381 gttcaaccag ctccgctcgg cgtacgggtt gttccgctgg gcacgagaaa cgggcaacaa
     7441 ggagagaatg gggagggcgc tcgcgacgag ggagcttctg ttccaaacac cgcagaaaaa
     7501 cgggcttttc cctgcaattg cggttctcgc gccagcggac ggacgcccgg ttgaatgggt
     7561 atcgagcagc cttcaaggtg gcggccccgg agtttaccat ctgcttgaca gctcgtggac
     7621 ggcgtactgg ttactgcagt ggcaacttga ctgcgaaccc gacgtgcgaa cactcccttt
     7681 ctgcacggcg tacgcggacg agcttgttcg tcttcagcgg agcgacggga gttttcccga
     7741 ctatgttgac gttgatacgc attcgtgtgt cacacattac gacccgaccg aacgcgcggc
     7801 gaggtccagt tccggttacg tacgtgcaat ggtgagcaga tggggcacga agcggctgcc
     7861 ggcgagtgcg gaaacagcga tccatgcgat gttcctcgcg gaactgagcc gaattgtggt
     7921 ggacggagaa cagtatttcc gttctgcgca ggacgccgct cgttacgtgg agcggaacgt
     7981 cttgccgttc cacaagtggt gggatttcga gacgtttttt tcatgttcac ccaaaccttt
     8041 gggttttttc gactcgcgaa gccagcagta tccgcagaac acgctgagcc ttttctgggc
     8101 ttctgaggcg ttccgaatcc tgtacgaaat cacgggaacc gcggcgatcg gcgagcaatc
     8161 cctggcgctt accgattatt tgtgcctgtt ccagcaggtg tggtctcctc cgttcctcag
     8221 tttctatgga ttcggcggtt tcggagtgat gaatactgat ggcgagtgga acgatgcgcg
     8281 acaggcgttg ttcgctgagg gccttgcgag gcaatatctc cagagcggaa ggcaggagta
     8341 cctcgagcga gccattgccg caaccagggc gggatttgcg tgtgcctaca tccctgagaa
     8401 tgcggatatt tgtccgagga ttttcgcccg gaagccaacc gggtatgcgg acgagaacta
     8461 cgcgcacggt ggttacgacg ctcctgcggg acccagcggg ttcgactggg gcatcggcag
     8521 tgcgcttacg accgcgattc ggatgctgga ctggttcgga gatttgtggg tggacgtgta
     8581 cgggggctgg gcgctgggca tcgacgcgct caccgttacg tcatgccggc gggaaggtga
     8641 tgtgtggcac ctgaccgtag atgcgccgtt gagacacacc gacactatca cggtgaaagg
     8701 taaccccggc gaaggagaac cagtttgcct ctcactgaac ggacagcaac ccgttgtgct
     8761 tacggacgaa gcgctgagga agggaacgac cgtgcgcctg taccctccgg gacaggatcc
     8821 gtgcactaga tgaaaaacgc ccccgcttca aatgaagcgg gagcgtgaaa tgagtagcgg
     8881 cgcagggact tgaaccccgg acacgcggat tatgattccg ctcaaaccca ccaactgcaa
     8941 caaccgacag taacccaaaa cacgtatgta tcgggcttaa gcggaatgct gttattgtga
     9001 caatttgtca tttgccgttg ttttttgctt tgaagtgtgc catcagtgtg ccatggcaca
     9061 atgcgtaagt gttggcgatc tgtggtctga ggggaggtgc attcacttct gggaatgtac
     9121 ggaacttcgg agaaaaaacc gttttggcac cgatctccga agtacccggt aatctacctc
     9181 gcggaaaccg acctaccccg cgcatctttg cccattacaa acggctccgg tgtacgcggt
     9241 gcgtccgttt ttccccgcaa gattgccatc agccgttcgt aacgttcctt catctctcct
     9301 ccggcgaact tgacccggca agcatccaac tcctcgagcg ccggtcgttg ttcccttgtc
     9361 agcccacaca tctggcaatg attctcttcg gaagcgcccg gcgaaaggtt cgcgctggct
     9421 cacccaggtt cgctcggctc acgcgggttc gcggcgcaga cggtcgatgg tgttgagaac
     9481 ggcgtttttc tgaagaccgg ggctgaacgg atgcccgtcg atctcagctt cggaatgccc
     9541 acacagcgcg atgagtgcag tgcgggcaac aacacgggct ttggcgacca tttccccttt
     9601 tggcggcttc atgtcgtgcg ccacgtcgtt ccgcgcgtgc cgcaaaccgc ccaactcatc
     9661 ccacccgcct gccgcatctt ccacagcaag ccaacgcttg tcggaaccct cgtgcgccac
     9721 gatcaacagg tccttcaaca acaggtcact ccgacggagg taatcgtcaa gggcattctc
     9781 cagcgtctcg acggcaagga gcaacgcggc gatcgggtcg tttcgcgcga tccgtctctc
     9841 cgctgaaaag aaggtgaaca tgcgcgcgtt tcgaggattg ttgtgcggga acgggtccag
     9901 ctgtcgcttc acaagctgcg cgactggttc gcacgccggg tggctattga gggacttcag
     9961 agccccttcc agccgctctt tgcctcccgc aaaatcccat ctgtcacacg cgtcccaccc
    10021 ttcgaggaca cgggcaagcg cgcgcatggc ctcaactgac tgggtctgag gagcagaact
    10081 gcagaagagc cgcagcgatc gcgccgcgcg tgagaactgg aacaggtccg ccgctcgttg
    10141 cacttcatcc aggatcgacg gccgccggaa gccgtgcagg ctgatctggc ggacgcgttt
    10201 cccatccgcc accggttgtt tggggtccat gacttgccat agtgtggcgt tgccgaggag
    10261 cccaagcatc gcaagcaccg accatgcctg cgccatctgc ggtgtcccgg acgacacccc
    10321 gacgtgaatc tcagcatccg cgggaaacga cgccgtttcc gcggtcagag cggcccgcat
    10381 cgcgtcgaaa acctcgtcgg cgatggcggg attgcattcc gtgaggcggc agatggagat
    10441 cgcttcagcc gcaatcccga ctgaatcgga aagccagaag gccgtctcat ccgcctttgt
    10501 ctcaaggctg tttgcgccag tcgtgacgaa caggctcacg cgttccggtt gttggccgat
    10561 ggccagcagg tgccgcgtga gagagactat ggcgccctcg ctcatgtcgg cctcggcgtg
    10621 cggatcgcgg ccccctacgc acgaaagcag aattctcatg gcaagccctc gtcgacggtc
    10681 cgttgccttt tgggaacggt attggccaat tcttcatccg gcccagaatt tacatccggg
    10741 aatgccttcc ggcaactcca attgcagaca cgactcggtt tcgagattgg aacgcggcgt
    10801 cattgctcgc catccgcctg gatgggttca gagaggatgg attcccgcct tcgcgggaat
    10861 gactgagcaa ccggcgccga ggcgtctgga gattcgtcag cggaccgcca tcggtgccgg
    10921 tcaccatggg gctgcgtcct ctgacgaagg caagcgggcc gacacactcg tgcgctggtg
    10981 cgcaggcggt cggcccctgc agaaaacggt gtgttccgat ccggtctacg tacccaccgg
    11041 gacatagtgc cgccaagcaa ttccaaacga ccccgccagg tttcgcgggg cgaactcaac
    11101 tcaggccacc tgacggtggc ttctgtaacc tcgcggtggc ccggatcgct tcacgctccc
    11161 cccaaaaagc taccccgtcg c
//
LOCUS       DAOSOO010000097        11087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5866_length_11087_cov_6.319616, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000097 DAOSOO010000000
VERSION     DAOSOO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_11087_cov_6.319616"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..819)
                     /locus_tag="PLT86_08180"
     CDS             complement(136..819)
                     /locus_tag="PLT86_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOT36627.1"
                     /translation="MPADLPERAAFKDRTLLFFSVFAAVLYAVGIAKYLHSPNDPLLS
                     ATTPWFLLVTGLPAFIISIPSERGVLYLAWGVVSCIFTLAVEIAGVKTGALFGSYEYG
                     KTLGPHILGVPPVIGFNWVIIVLGFSGVCSRLPCGAACKAFLSAASATGFDWVMEPVA
                     IALGYWKWEQNCIPLQNYAAWFFVSLVMSAAYVHLRVTWRNPAPFIIVFAQLVFFAVL
                     RVSLGLSAC"
     gene            complement(821..1870)
                     /locus_tag="PLT86_08185"
     CDS             complement(821..1870)
                     /locus_tag="PLT86_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT36628.1"
                     /translation="MNAGYFGSRSEEIKAFLRSHLEACVEEFSFLNRWGKDLPARIGA
                     FARGGKFIRGGLVFLGHELAGGRPGETTLRIAAAIELLHSAFLIHDDIMDQDRMRRGM
                     PSVFWQFREEAHAKGIEESEHYGTSMGICAGDCALFIALRLISSADIPDGVKDLVCRE
                     SYAIGLAQMADIDYGTFSRIPSEKEIIDLYLWKTARYSFSLPLSAGAMAGNADEELVS
                     ALWRLGESMGIMFQMRDDELGIFGSENAIGKPVGSDIRLAKKTLLIAIALKRLDPVSR
                     SELTGILGRRDATADEILRARRLIETSGARSELISRMNALSSETGTMIDALVAPEDSK
                     ERLRSLVDFIRTRDR"
     gene            complement(1867..2733)
                     /locus_tag="PLT86_08190"
     CDS             complement(1867..2733)
                     /locus_tag="PLT86_08190"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOT36629.1"
                     /translation="MKRTQSAFEEIFRRGSRTFFNSSLFFPEKVRRDVVILYAFVRTA
                     DDFVDSIPQDKEGFHAFRARYELARRTGRDSGDPIVDSFIELSRRRKFDEAWAEAFLN
                     SMEADLTTKRYETPDEVLVYIHGSAEVIGFFMASIMELPSTAMEAAAFLGRAMQYINF
                     IRDIAEDNELGRTYLPLAGSGLEDLTEDTARSKPEVFARFIRSQIDLYREWQAHAEAA
                     FGWIPWRSRVAVMTAGEMYKWAATKIYRNPFIVYEKKVKPSRQRIFFSLLRNASRAHR
                     CRRPEAPDQEPA"
     gene            2784..3878
                     /locus_tag="PLT86_08195"
     CDS             2784..3878
                     /locus_tag="PLT86_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36630.1"
                     /translation="MMVLIACIVIAFTALQFLVALANLIFESDLPEEGEKEGPLVSVL
                     IPARNEEKNIGNIIEDLLGQDYQHIEIVVFNDQSSDRTEEVVRTYAVRDERIRLINST
                     GLPAGWTGKNYACHSLALNAKGEYFLFLDADVRISGNVIGRCLALVRKKGLDLVSVFP
                     KQEMQTPGEKITVPIINYVLVTLLPLVLVEKSCHSSLAAASGAFMFFTAGAYREVLPH
                     EKVKRAVVEDVSIARLFKKADKRVACLLGDDDYSCRMYRGFRHAVGGFSKFITTFFAD
                     SFVLSVLFFLVTTFGFLPVLWAMPSWVFAGYLVVYVLTRVIVFFLSHQNILSSVLLSI
                     PVQLSLGLINCMAYLSRFSGKLEWKGRQIA"
     gene            3936..5414
                     /gene="crtI"
                     /locus_tag="PLT86_08200"
     CDS             3936..5414
                     /gene="crtI"
                     /locus_tag="PLT86_08200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT36631.1"
                     /translation="MKKKVLVVGAGLGGLATALRLAKKGYEVEIVEKNSNAGGRLNQI
                     KKDGFTFDTGPSFFAMSYEFEDFARECGIALPFEHYALDPLYSVNFRKDRVTYHLHRD
                     IGKLASQFKHVEPDFENRMRRYLDKCGQVYRDTNFVIKTNYDSLPDYLSALAKANPFL
                     LPLLWRSFWKQVSRYFDSNDAREIVSLVSFFLGKTPFDTSAVYTLLSYTEFVHDGYYN
                     VVGGMYRIAEGVVRELEKENVRIHYGVEIVDFVAKDRTVEYLLDGEGRKWTADILVVN
                     ADAAVFRGRVFRRRKYSPEMLDRMDWTMGTLTIYLGIKCKLPQLNHHNYYLGDDFTEY
                     AHRVFNDPSAAEKPYYYVNVLSRTNPDCAPEGCESLFFVCPVPDLRYKSNWDDRDAIV
                     DGIIDDFSNRTGRNIRNEIVSRTVYTPIEWRDRFNLHRGSALGLAHNMMQTGGFRPKN
                     FDEEFGNVFYVGASTVPGGGLPMVVIGSKLVTERVERFCGNP"
     gene            complement(5503..5742)
                     /locus_tag="PLT86_08205"
     CDS             complement(5503..5742)
                     /locus_tag="PLT86_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOT36632.1"
                     /translation="MADSVVFDRKPAVKELEPGSYAWCRCGRSKSQPFCDGSHKGTGI
                     QPVVFKLEEKKKAALCLCKQTKTPPFCDGTHRTVT"
     gene            complement(5818..6726)
                     /locus_tag="PLT86_08210"
     CDS             complement(5818..6726)
                     /locus_tag="PLT86_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825803.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT36633.1"
                     /translation="MERYRSFSRVLKDQFGRPVRKISLDAGFSCPHRKGSEGGCTYCD
                     AWGSGTGASRQGRSISDQIAAAISREAAARKPAGAFLAYFQAHTNTFAPVPTLRSRFE
                     EALTDPRVVGLSIGTRPDCLPEPVLDLLDELNRRTCLWVELGLQSAHDETLRRVNRGH
                     TVAQFADAVERCHARGIRVCAHVIFGLPGETREMMHDTARFLASLGIDGVKFHSLYLV
                     PATQMEREYRRVPFPLLSQQEYAEIVADALERLPPEAVIQRLTGDPPRTIPAEPPWTR
                     DKVGTLALIRRELARRDSFQGALYRG"
     gene            6768..8378
                     /locus_tag="PLT86_08215"
     CDS             6768..8378
                     /locus_tag="PLT86_08215"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOT36634.1"
                     /translation="MQKYTLETCRKRTSGVGARTTVMALKGLRMAELRSQWGSKVGFV
                     LAASGSAIGLGNIVFFSANAYKYGGGAFYIPYIVAFCILGIPIMLMELALGAHTGKAF
                     PQSLYRIGKRPAEFIGWWGVLNATVICMYYVAILAWVLGMFIGSMKGLWSSEPVTSGA
                     FGSLALPNPFGYFFGMLGSWDTLWYVAIVWALNLLIVLRGARSIESATRFLVPLMWLC
                     MIALIVRGVSLPNGEQGAYLLFSPNFTVMADPEVWKGAFSQMFFTLSLGFGIMTAYAS
                     YLPKKSDQVHNAATICFLNCSFEFIAGLAVFSLLFAFSVVPKASTLSMMFFVVPQGIA
                     KLPHFVVGFGMLFFFLLFIAGLTSSISLVEAFVASILDKFRWRRKKAVLVVALCSMLG
                     SMCFALPHVVDPTLASNGTLGLTLLDLFDHWSFSYGLLIVGFFECLIIGWVFGARKLR
                     EIINRDSTLGIGWWWDALIKVVNPVIIAVVIGWGVWTEVRDGLYGASFTIQGIPWLSW
                     FAFGAWFLFTTGLALVFTFGRKYREEQG"
     gene            8375..8875
                     /locus_tag="PLT86_08220"
     CDS             8375..8875
                     /locus_tag="PLT86_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36635.1"
                     /translation="MNRLVFWVPAFAFISVSTARGIVLSVEPASPVRGEVAAIRVFGD
                     ATADTSLAAREAARTGWKVYVEYFPNSKVVRDDTLGVTGPAGEVFWEPAFAGIVAVRA
                     IRKKEGGSPEQLATNVSVRFPSVPAGGVLVFLTAGFVLLGGAGWAFVHLLEHKPPGEG
                     RSEPEA"
     gene            complement(9142..10267)
                     /gene="prfB"
                     /locus_tag="PLT86_08225"
     CDS             complement(join(9142..10209,10211..10267))
                     /gene="prfB"
                     /locus_tag="PLT86_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT36636.1"
                     /translation="MPLVNTDRLTELRERLDSLGGFFDLARKREQISILEQQMAAPGF
                     WDDGQAAQKTISAANELKEWIQDWESLDRRLNDLAELAELAELEEDDAVAAEVERDTE
                     TLANDITALEFRHMMSDPADKKNAIVTIHPGAGGTESTDWAQMLMRMYTRWAERKGFQ
                     VEIMDLLENEDAGIKSVTLEVTGPYAYGYIKAESGVHRLVRISPFDSQKRRHTSFASV
                     FVYPELDDEIKLDVKESDIRVDTYRSSGAGGQHVNKTSSAVRMTHLPTGIVAQCQNER
                     SQHKNRANCWKILMGRLYEHYKALEDEKLQAVEDTKKPIEWGSQIRSYVFQPYQLVKD
                     HRTGIETSNVDAVMDGDIDLFINGYLTRKGDLTPDDSLAQ"
     gene            10609..11087
                     /gene="carB"
                     /locus_tag="PLT86_08230"
                     /pseudo
     CDS             10609..11087
                     /gene="carB"
                     /locus_tag="PLT86_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985177.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
BASE COUNT         2246 a         3155 c         3218 g         2468 t
ORIGIN      
        1 ttccgcacac tcatactacg tgaaccgtcc gggttcctcc cgtttccccg aaggagaacc
       61 cacctcactg ttttgaccgt gaaacaacgt ctcttccgcc cgcgccccca ggtaatcgag
      121 ggagacgaat ctccctcagc acgcactcaa ccccagtgac actctcagaa ctgcaaaaaa
      181 aacaagctgc gcgaacacga tgatgaaggg agcaggattc ctccaggtaa ccctgaggtg
      241 cacatatgcc gcggacatga cgagtgacac gaagaaccat gctgcgtagt tctggagagg
      301 gatgcagttc tgctcccatt tccagtagcc caacgcgatg gccaccggtt ccatcaccca
      361 gtcaaacccc gtggctgacg ccgctgaaag aaacgctttg caggctgctc cgcagggaag
      421 gcgcgaacac acgccgctga aacccagaac gataatcacc cagttgaacc ctatgacggg
      481 cggcacgccc agaatatgcg ggccgagggt cttcccgtat tcgtacgagc caaaaagagc
      541 tccggtcttg acgcccgcaa tctcaacggc aagcgtgaaa atgcaggaaa cgacgcccca
      601 tgcaaggtag agaactcctc tttctgacgg gattgagatg atgaacgcgg gcaggccggt
      661 caccagcaga aaccagggcg ttgtggcgga taagagagga tcgttcggtg agtggagata
      721 tttcgcgatg ccaacggcgt acagaacagc tgcgaaaaca gagaagaaca ggagcgtgcg
      781 atccttgaaa gccgcgcgtt cagggagatc ggcaggcatt tcaacggtcc cttgttcgta
      841 tgaaatcaac gaggcttctg agacgttcct ttgagtcttc cggagccacg agagcgtcta
      901 tcatcgttcc ggtttccgag gagagcgcgt tcattctgga aatcaactca gatcgggcgc
      961 cgcttgtttc tataagccgg cgggcgcgca ggatctcatc cgcggtggcg tcgcgtcgtc
     1021 cgagaatgcc ggtcagttct gagcgtgaga cagggtcaag ccgcttgagg gcgatcgcga
     1081 tgagaagggt cttttttgcc agcctgatgt cagaacctac gggcttcccg atcgcattct
     1141 ccgagccgaa gatgccaagc tcatcatccc tcatctgaaa catgattccc attgattcgc
     1201 caaggcgcca gagcgctgag acgagttcct cgtccgcatt tcctgccatt gctccggccg
     1261 aaagtggcaa agagaaggaa taccgcgctg ttttccacag gtagaggtca attatctcct
     1321 tttcggaggg gatgcgggag aaggtcccgt aatcgatatc cgccatctgc gcaagaccga
     1381 tcgcgtaaga ttcgcggcat acgaggtcct tgacgccgtc cgggatatcc gccgatgaga
     1441 tcaaccgcag cgcgatgaac agggcgcagt cgccggcgca gatacccatg gaggtgccgt
     1501 aatgctctga ttcttcaatg ccctttgcgt gggcctcttc gcggaactgc cagaacaccg
     1561 acggcatgcc tctcctcatg cggtcctggt ccatgatgtc gtcgtgaatc aggaatgccg
     1621 aatgcagcag ttcaattgcg gcggcgattc tgagcgttgt ttccccgggc cggcccccgg
     1681 ccagttcatg gcccagaaag acaagacctc cccttatgaa cttcccgccg cgtgcaaacg
     1741 ccccaatgcg cgcgggcaga tcctttcccc accgattcag gaaggagaat tcctcgacac
     1801 aggcctcaag gtgcgatcgg agaaacgctt tgatctcttc agaacgggat ccgaaatatc
     1861 ctgcgttcat gcgggttcct gatctggagc ttcgggtctt ctgcagcgat gagctcttga
     1921 cgcgttccgc aacagcgaaa aaaagatcct ctgtcgggaa ggtttgactt tcttttcgta
     1981 cacaatgaag gggttgcggt agatctttgt tgccgcccac ttgtacatct cgccggccgt
     2041 cataacggct actcgagacc gccacgggat ccagccgaac gcggcttcgg cgtgtgcctg
     2101 ccattctcga tacaggtcta tctggctgcg gatgaagcga gcaaacacct cgggcttcga
     2161 tcgggcggta tcttccgtca ggtcttcgag gcccgacccg gcaaggggca gatatgtcct
     2221 gccaagttca ttgtcttccg cgatatcgcg gatgaagttt atgtactgca tggccctgcc
     2281 gaggaaggct gcggcttcca tcgcggtcga ggggagttcc atgatggatg ccatgaaaaa
     2341 cccgataacc tcggcggagc catgaatgta gacaagcacc tcgtcgggag tttcgtacct
     2401 ctttgtggtg agatccgcct ccatggagtt caggaaggct tcggcccacg cctcgtcaaa
     2461 cttccttctc cgggaaagct cgatgaacga gtctactatc gggtccccgc tgtctctgcc
     2521 agttctcctg gcgagttcgt agcgcgcgcg gaacgcgtga aatccttctt tgtcctgggg
     2581 gattgaatca acgaagtcgt ccgctgttcg cacgaaggca tacagaatga ctacgtccct
     2641 ccggactttc tccggaaaga agagggaaga gttgaagaag gttctggacc ctcttctgaa
     2701 gatttcctca aatgccgatt gcgtacgttt catagcgact tcaacgtgcc gaaccgcagg
     2761 tcttccgagg aactgagaac ggaatgatgg tcctgatagc ctgtatcgtg atagccttca
     2821 ccgcgctcca gtttctcgtg gcgctggcta atctcatttt cgaatctgat cttcctgaag
     2881 agggggaaaa agaaggtccc cttgtgtccg tccttatccc tgcaagaaat gaagaaaaga
     2941 acataggcaa tattatcgaa gacctcctcg gtcaggatta ccaacacatc gagatagtgg
     3001 ttttcaacga tcagtcttct gacagaacgg aagaggttgt cagaacatac gcagttcgtg
     3061 atgaaagaat aaggctgatc aattccacgg ggcttcctgc gggatggacc gggaagaact
     3121 acgcatgcca ctctctcgcg ctgaacgcga agggggaata ctttctgttt cttgacgcgg
     3181 atgtgaggat atccggcaac gtgataggca ggtgtcttgc tcttgtgcgg aagaaagggc
     3241 tggacctcgt ctcggtgttt cccaaacagg aaatgcagac gccgggcgaa aaaatcacgg
     3301 tgccgataat caactatgtg ctggttactc tcctgccgct cgtactggtc gaaaaaagct
     3361 gccacagctc gcttgcggcg gcaagcggcg cgttcatgtt tttcacggca ggggcttacc
     3421 gggaggtact ccctcacgaa aaggtcaaac gcgcggttgt agaggacgta agcatagccc
     3481 gcctgttcaa aaaggcggac aagcgcgtag cctgcctgct cggcgacgat gactattcgt
     3541 gcaggatgta ccgtggtttc cgccatgcgg taggcggttt ctcaaaattc attaccacgt
     3601 ttttcgcaga ttcgttcgtt ctctccgtgt tgttcttcct cgttacgacg ttcggttttc
     3661 tccccgtcct gtgggcaatg ccgtcctggg tcttcgcagg gtatctggtt gtgtatgttc
     3721 tgacgagggt cattgtgttc ttcctcagcc atcagaacat cctcagcagc gtgctgttga
     3781 gcattccggt gcagctctct cttggcttga taaactgcat ggcgtatctc agcagatttt
     3841 ccggaaaact cgaatggaaa ggacggcaga tagcctgaga tagacagccg ttcgcggtgg
     3901 ttctgcgttc gtgattaata gatgagaggc gggacgtgaa aaagaaggtt ctggtggtcg
     3961 gagcggggct cggaggcctg gcaacggcgc ttcggctcgc aaaaaaaggc tacgaggttg
     4021 agatcgtcga aaagaacagc aatgccggcg gcaggctgaa ccagatcaag aaggatgggt
     4081 tcacgttcga taccggtcca agttttttcg cgatgtccta cgagtttgag gatttcgcga
     4141 gagaatgcgg catcgcactt ccctttgagc actacgcgct tgacccgctg tactcggtca
     4201 atttcagaaa ggaccgcgtt acctatcatc ttcaccggga tatcggcaaa ttggcgtccc
     4261 agttcaaaca tgtggaaccg gatttcgaga acaggatgag gaggtatctc gacaaatgcg
     4321 ggcaggtcta ccgggacacg aacttcgtga tcaagacaaa ttacgattct ctgcctgact
     4381 acctctccgc acttgcaaaa gccaatcctt ttctgctgcc cctgctctgg aggagtttct
     4441 ggaaacaggt gtcccggtac ttcgactcaa acgacgcgcg agagattgta tcgctcgtgt
     4501 ctttcttcct tggcaaaacg ccgttcgaca cctccgccgt gtacacgctt ctttcttaca
     4561 ccgagtttgt gcatgacggg tactacaacg tggtcggcgg gatgtacaga attgcggaag
     4621 gcgtcgttcg cgagctcgaa aaagaaaacg tcagaataca ttacggcgtt gagatcgtgg
     4681 actttgttgc gaaggacagg acggttgagt atctgctgga cggagaaggc aggaaatgga
     4741 cggcggatat tctcgtcgta aatgccgatg ctgcggtatt tcgaggccgc gtgttcagaa
     4801 gaaggaagta ctccccggag atgcttgatc gcatggactg gaccatgggg acgctgacga
     4861 tctatctggg catcaaatgc aaattgccgc aactgaacca ccacaactac tacctcggtg
     4921 acgatttcac cgagtacgca cacagggtgt tcaacgatcc gagtgcggcg gaaaaaccct
     4981 attactatgt gaacgtgcta tccagaacga atcccgactg cgctcccgag ggctgtgaaa
     5041 gcctgttctt cgtctgcccc gtgccggatc tgaggtacaa gagcaactgg gacgacaggg
     5101 acgcaattgt ggacgggatc atcgacgatt tttcaaacag gacagggaga aacatcagga
     5161 acgagatcgt atccaggact gtctacacgc cgatcgaatg gcgcgacagg ttcaatctcc
     5221 acagagggag cgcgctgggt cttgcgcaca acatgatgca aacaggggga ttcaggccca
     5281 agaacttcga tgaagagttc gggaacgtct tctacgtggg agcgtcaaca gtcccgggtg
     5341 gcggacttcc aatggtggta atcggctcga aactcgtaac cgagagagtt gagcgttttt
     5401 gcggaaaccc ttagggcgac tgataccgcg gtactccaaa aactacttga gggacgcggg
     5461 gcaggccgcg tccctttttt gtactcattc gggaaagttg cgctacgtta ccgttctgtg
     5521 cgtaccatcg cagaacgggg gcgtttttgt ctgcttgcac aggcagagag ctgccttctt
     5581 cttctcttcc agcttgaaaa ccaccggctg gatccctgtt cccttgtgcg atccatcgca
     5641 gaacggctgg cttttcgacc gaccgcacct gcaccacgcg tacgagccgg gttccagttc
     5701 cttcaccgcg ggttttctgt caaacaccac tgagtcagcc atgccggttc ctccgaaaac
     5761 acgtgatacg cctggataat ggaatcgtgg aaggtgagtc actgaaaata tagggtctta
     5821 cccccggtaa agcgccccct gaaaactgtc ccgtctcgcc agttctctgc ggatcagggc
     5881 aagcgttccg actttgtccc gcgtccacgg tggctccgcc ggaatggtgc ggggaggatc
     5941 cccggtgagc ctctggataa cggcctccgg cggcagccgc tcaagggcat ctgcaacgat
     6001 ctccgcgtac tcctgttgcg acagaagcgg gaacggaacc ctccggtact ccctctccat
     6061 ctgcgttgcc gggacgaggt agagcgaatg gaatttcacc ccgtcaatac ccagggacgc
     6121 gagaaaccgg gcggtatcgt gcatcatttc ccgcgtttcc cccggcaacc cgaaaatcac
     6181 atgagcgcac acgcgaatgc cgcgcgcatg gcatcgttcc acggcgtcgg caaactgggc
     6241 caccgtgtga ccgcggttga ccctccgcaa cgtttcgtcg tgcgcggact gaagcccgag
     6301 ctccacccag aggcaggtgc ggcgattcag ttcgtccaga agatccagaa ccggctccgg
     6361 caggcaatcg gggcgcgttc cgattgacaa cccgaccacg cgcgggtcgg tcagcgcttc
     6421 ttcgaaccgg ctgcgcaggg ttggaactgg cgcgaaggta ttggtgtgcg cctggaaata
     6481 tgcgagaaac gcgcctgcag gcttccgcgc cgcggcctcg cgcgaaatcg cggcggcaat
     6541 ctggtcggag atggagcgac cctgtctgga tgcgccggtg ccggaacccc acgcatcgca
     6601 gtaggtgcat cctccctcgc ttccctttcg atgcgggcag gaaaaacccg cgtcgaggga
     6661 gatcttccgc accgggcgtc cgaactgatc cttcagcacg cgcgaaaagc tgcggtatcg
     6721 ttccatcgtt cctgcaacgg aaaagggcaa ggattgggac gaaaatcgtg caaaagtata
     6781 ctcttgaaac gtgccggaaa agaacgtccg gtgtcggcgc acgcaccacc gtcatggctc
     6841 tgaaaggtct ccgtatggcg gaactacgtt cacagtgggg ttccaaggtc gggtttgttc
     6901 tcgcggcatc tggttcggcc ataggcctcg ggaacatcgt cttcttcagc gccaatgcgt
     6961 acaagtacgg cgggggagcc ttttacatcc cgtacatcgt ggcgttctgc atcctcggca
     7021 ttcccatcat gctcatggag cttgctctgg gagcgcatac cggcaaggcg tttcctcagt
     7081 ccctctacag gatagggaag cgcccggcgg agttcatcgg atggtggggc gttttgaatg
     7141 ctacggtcat ctgcatgtac tacgtcgcca tcctggcctg ggttctcgga atgttcatcg
     7201 gcagcatgaa agggttgtgg agttcggagc ccgtcacgag tggcgccttc gggtctctcg
     7261 ccctccccaa tccgttcggg tatttcttcg ggatgctcgg ttcgtgggat acgctgtggt
     7321 acgtggcgat tgtgtgggcc ctcaatctcc tgatcgttct gcgcggtgcg cggagcattg
     7381 aatccgccac ccgttttctc gtgccgctca tgtggctgtg catgatcgcc ctgatcgtcc
     7441 gcggggtgag cctgccgaac ggagagcagg gcgcatacct gctgttcagt ccgaacttca
     7501 ccgtcatggc cgacccggag gtgtggaaag gggccttcag ccagatgttc ttcaccctgt
     7561 cgctcgggtt cggcatcatg acggcgtatg cgagctatct gccgaaaaag agcgatcagg
     7621 ttcacaacgc ggcaaccatc tgctttctca actgctcatt tgagttcatt gcagggctgg
     7681 ccgtgttctc ccttcttttc gcgttctctg tggtgccgaa agccagcact ctcagcatga
     7741 tgtttttcgt ggtgccccag ggtatcgcga aattgccgca ttttgtcgtg ggtttcggga
     7801 tgctgttttt cttcctgctg ttcatcgccg gcctcacctc cagtatttcc ctcgtggagg
     7861 cctttgtcgc gtccattctc gacaaattcc ggtggaggag aaaaaaagcc gtactggtcg
     7921 ttgcgctgtg cagtatgctg ggtagcatgt gcttcgctct tcctcacgtg gtagatccca
     7981 cccttgccag caacggcacc ctcggcctca cgctcctcga tctgttcgac cactggtcgt
     8041 tcagttacgg cctcttgatt gtcgggtttt tcgagtgtct catcatcggg tgggtgttcg
     8101 gtgcacgcaa actgcgcgaa atcatcaacc gcgacagtac gctcggcata gggtggtggt
     8161 gggatgcgtt gatcaaagtg gtcaatcccg tcattatcgc ggtggtgatc ggatggggcg
     8221 tctggacgga ggtgcgggac gggctctacg gcgcttcatt cacgatccag ggaataccct
     8281 ggctttcgtg gttcgcgttc ggggcgtggt tcctcttcac cacgggtctc gccctcgtgt
     8341 tcaccttcgg gcggaagtac cgggaggaac agggatgaac cgactggtat tctgggttcc
     8401 cgcattcgcg ttcatcagcg tttcaacggc gcgcggcatt gtgttgagcg tcgaaccggc
     8461 gtctccggta cggggcgaag tcgccgcaat ccgggttttc ggtgatgcca cggccgatac
     8521 ctcactggcc gcccgggaag ccgcccgtac cggctggaag gtttacgttg agtacttccc
     8581 caacagcaag gtggtgcggg acgacacgct cggcgtcacc ggtccggcgg gagaagtgtt
     8641 ctgggaaccc gcattcgcag ggattgtcgc ggtgcgggcg ataagaaaga aggagggtgg
     8701 ctcccccgaa caactggcca ccaacgtaag cgtgaggttc ccctccgttc cggcaggtgg
     8761 agttcttgtg tttctcaccg ccggattcgt cctccttggc ggtgccggct gggcgtttgt
     8821 tcaccttctg gaacacaaac cgccggggga ggggcggtcg gaacccgaag cgtaagcccc
     8881 ctgccgcccg gcaagcccgc gccgggcacc gcgccgtcat tcccgcgaag gcggggatcc
     8941 agccggtgcg cctttttttc cttccgccac aaaaggttcc ggaatccgtt tccctcgcgg
     9001 ccggggcgcc gccgaattgc ttacttaatg cccgcggagt agtcccggat tcccgcctcc
     9061 gcgggaatga ctatcgtggt gcctctccga ctgcgcgccc gtcgaatcag atccatgcgc
     9121 cccggtgcca gacctcgcct gctactgcgc cagcgagtca tccggcgtca gatccccttt
     9181 gcgagtcagg tatccgttga tgaacagatc aatgtctccg tccatgacgg cgtccacgtt
     9241 ggatgtctcg atgcccgtgc ggtggtcctt gaccaactgg tacggctgaa acacgtacga
     9301 ccgtatctgg ctgccccatt cgatgggctt tttggtgtct tcgaccgcct ggagtttctc
     9361 gtcttccagc gccttgtagt gctcgtacag gcgacccatg aggattttcc agcagttcgc
     9421 gcggttcttg tgctgcgagc gctcattctg gcactgcgcg acgataccgg tggggaggtg
     9481 ggtcattcgt accgcggagg aggtcttgtt cacatgctgc ccccccgcgc cggacgagcg
     9541 ataggtatcc acccgaatat cggattcctt cacgtccagc ttgatttcgt cgtcgagttc
     9601 cgggtacacg aacacactgg cgaatgaggt gtgccggcgc ttctgggaat caaaaggaga
     9661 gatgcggacg agccggtgca cgccgctttc ggccttgata tacccgtagg cgtacggccc
     9721 ggtcacctcc agcgtcacgc tttttatgcc ggcgtcctcg ttttccagca ggtccatgat
     9781 ctccacctga aagcccttgc gctccgccca ccgggtgtac atccgcataa gcatctgcgc
     9841 ccagtcggtg gattcggttc cgccggcgcc ggggtggatc gtcacaatgg cgtttttctt
     9901 gtcggcgggg tcggacatca tgtggcggaa ttcaagcgcg gtgatgtcat tcgccagcgt
     9961 ttcggtatca cgttcgacct ccgccgccac tgcgtcatcc tcttccagct ccgccagttc
    10021 ggcaagttct gcaaggtcgt tgagtcggcg atcgagtgac tcccagtcct gaatccattc
    10081 tttgagttcg tttgcggcgg agatggtttt ttgtgcggcc tggccgtcgt cccagaatcc
    10141 cggtgcggcc atctgctgtt cgaggatgct tatctgttcc cgtttgcggg cgaggtcaaa
    10201 gaaacctccc gaggctgtcg aggcgttcgc gaagttcggt aagccgatca gtgtttacca
    10261 gtggcatgtg tcaatcctcg tggtgaatca agaggtgaat cgtccgcgtc ccggggttct
    10321 cgctcgttcc aaaccctttt ccacggaaac ccgcggcacg tacagaaggt acgtcgcgcg
    10381 ggcctgcaga gcacgtccgg aaactgctcg ttgacgcttc atgcctcctg aggtaccttt
    10441 agatgccctg gtttttcgcc ctttgcgggc ctgtgctgcg ttctcgcccg cgctctcgct
    10501 ttctttcagg ttgccaccat gccggttcca gattcccccg tagagcggct caccgcacgc
    10561 atcccacgcg cgacgcttct cagggccaag cagatggggt tcagcgacat acagcttgcg
    10621 cacctttgga acgtcgatcc tgcggacgtg cgcgagcttc ggaagaagct cgacgtcctc
    10681 ccacgtttaa aacggtggac acctgcgcgg cggagttcga ggcccgaacc ccgtaccact
    10741 acagcacgta cgaaaccgag tgtgaagtag tccgttctcc gaaaaagaag atcatgattc
    10801 tgggtggcgg accgaatcga ataggccagg gcatcgagtt cgattactgc tgctgccacg
    10861 cggcgtttgc gctgcgggaa gacggtttcg aaaccatcat ggtcaactcg aacccggaaa
    10921 ccgtttcgac cgactacgat acctccgaca agctctactt cgagccgctc accgttgagg
    10981 acgtgctgaa catccatgac agggaacaac ccgacggcgt aatagttcag ttcggcgggc
    11041 agacgccgct caaactcgcc gttcctctgg agaaggcggg agtaaaa
//
LOCUS       DAOSOO010000098        11002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_5928_length_11002_cov_5.788527, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000098 DAOSOO010000000
VERSION     DAOSOO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5928_length_11002_cov_5.788527"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..2379)
                     /locus_tag="PLT86_08235"
     CDS             complement(73..2379)
                     /locus_tag="PLT86_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36637.1"
                     /translation="MRPRILALFLAGVIAASAIPVRAQVLPPDSSVAVRAFPDIVAAL
                     EARLLALRDTASARGDSMLLRQVDLLSSELITAQAMFASGDILGAQVRISEIAAKVRV
                     LWSSLAPLPPPVPIPGDSVVILPPYPGPPLPPIEPVPVDIRELVQKAERLIVFLRGLA
                     TQQGDTTALAIIDRSEAIFRNLLASGTLANDEPTRAMLWGIISTLERLLGQLQPLPPP
                     FPPDSSIVIVPPDPGLPYPPPDSVIIMPPFILPDSAAIAGRLDRIQAFLDSAVAVVRS
                     EPNLPASEMIPRAYAVIDSARDYLSEGAKQRAFDLTTRAGILAGRIREALAVRARVET
                     GVDLVRQHADSLALFLSLNPNARASEALAPVPAMLDSVRLLLDEGRVFRADAVLQRAR
                     ERLRLAHEMIALEAKLRRMCGELTQRIERVGPIVEASGDTVALALFERAKTAADSAGK
                     ALEEGAFRRAEALIGASNMALDRALFVATAPDRIAGEIAALRTRIARLLSSLPATAVR
                     ERAIVTRASALVDSAQVALNARLYPKAEQMLVKVRYALDTLLRTPIIDPVIPPVTTLP
                     LDSLVALARQWAAQAVVLNDSARSDSVRVILERVVVLLDQVSAALAEGNREEAVELAN
                     NALILAASALRTARADLVDDWSADPVVKDLIAAGEEAERAVQAPTEVDKGAATLQYTL
                     KNTPNPFNPSTTVRYSLGATGHVNLAVYNMLGQEMRVLVNGVRSAGSHEVTWDGRDAN
                     GIPLASGIYFARLQTESTTLVIRMVLSR"
     gene            2716..3447
                     /locus_tag="PLT86_08240"
     CDS             2716..3447
                     /locus_tag="PLT86_08240"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36638.1"
                     /translation="MTGSMRSMCAVLAGAVLGAIVPVAGASPVSITADVSGVSHYFWR
                     GYDLLGGAKLPVQPALTATAENGLSAGIWGSAALLDRSTTDAADELDFTVSYSRPVVP
                     GIEASAGGTYYYLPNNTIASTAELFASAAFVEIPFEPTLTAYYDTKWLDDSSAEADGW
                     YFLAAASHSVPVGRFSLDLSAGLGFGSNSTFTGPQDLTLRAATTIPVGRVKLTPFLSP
                     ILVFEDAVNSDTFGICGGVSVSYAF"
     gene            3982..5529
                     /locus_tag="PLT86_08245"
     CDS             3982..5529
                     /locus_tag="PLT86_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOT36639.1"
                     /translation="MEYYEEANARVALGLRRPEPGDWYHGYPEYHVERDDDKCIRCLV
                     CVRQCPYDCTFYDPHRHGVYNVHENCVNCRRCEFMCPTSAIRITKWGAGFRDNDNWKQ
                     KTIRTVYKQADSGAVSLTGLGNDREYVSYFDRMVIDASQVTNPSIDPLREPMELRAYI
                     GKKPERHTNPPEVYPNLKAELPVLFAPMSFGAIAFNFQRGLAMAAKEVGTYFNTGEGG
                     LHPDLRQYGDHAIVQIASGRFGVSLDYLSNAAACEIKIGQGAKPGIGGHLPGEKVTWD
                     VSRTRMIPEGSDALSPAPQHDIYSIEDLRLLIYGLKEATEYTRPVSVKIAAVHNVAAI
                     ASGIVRAGADIVVVDGFRGGTGAAPTVIRDHVGIPVEVALAQVDQRLREEGIRHQASL
                     IAAGGIRSSADVIKGLALGADAFYIGTAAMVAVGCTACQQCHRGRCSWGITTNRPDLA
                     PRLQPEVAAERLTNLLHGWAHEIKEMLGMMGLNSIESLRGNRLKLRGVGMTQVELDTL
                     GIQQAGL"
     gene            5532..6068
                     /locus_tag="PLT86_08250"
     CDS             5532..6068
                     /locus_tag="PLT86_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT36640.1"
                     /translation="MKRVYPREERCINCHLCEVACIVEHSETKLPAAAYHVEGLRFNR
                     EDSRTICDPAEAGTAGVPKPLNRTLVQTAGYLSLSTNCRHCEEPDCVLACKNGSLYVA
                     EDGRVLVQEDKCVGCWMCVMACRYGAISRNPARSNVPAVAFNGINHHCDLCPEREVPA
                     CVWVCPTNALVFEDRDDQ"
     gene            6065..7321
                     /locus_tag="PLT86_08255"
     CDS             6065..7321
                     /locus_tag="PLT86_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36641.1"
                     /translation="MNHVIIGNSFAALFAIEAIRRTDQDAEITVISREPQHVYSRAMI
                     HEMLAGMVREPMVYLRDWDFYKRMRVRTMLGRTVTKINPGLQTVSTEEDETPYDRLLI
                     ATGGKPFVPPLNGLQDITYHSFITLEDAKKLKAACENRRNAVVLGAGLIGLQCAEALR
                     HMGLNVAVVEMADTVLPMALDTESSQTIAKELADEGIAVHTGDTIDLIISNNGHPTEC
                     KLRSGGTIPCDLLVIAVGVRPNIDLAKDAGITTDRGIVVNEHMETSAEHIYAAGDCAQ
                     GPEIVTGITMPIPVIPVASTQGMIAGYNMAGKRRRYGGALALNAMQFGGVQVISYGFV
                     KDERDGEVLKYVDPPRKIYRKVIIKNGRITGAIFVKDVDRAGIFRHLMEKRIDVTPFK
                     EHLLSPHFGVAHLPAEIRADMFTRPQ"
     gene            7359..8510
                     /locus_tag="PLT86_08260"
     CDS             7359..8510
                     /locus_tag="PLT86_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HOT36642.1"
                     /translation="MPSMRGNRHFVRRPHQEKDISACGIIGFINRDGTRVSGDAIIRS
                     IANMHDRGNGLGGGFAAYGIYPEHADAYALHIMYDDERAIADTEALIRNTLETEHAET
                     IPVHPNGTVTEYPEFRRYFCHPPDVDERDADEYMVRLVMRINAKIEGAYVVSSGRNMG
                     AFKGVGFPEDLAEFFCLQEYEAYLWTAHNRFPTNTPGWWGGAHPFTLLDWSIVHNGEI
                     SSYGINRRYLESFGYACTLRTDTEVVAYIFDLLVRRHGLSFEQAATVMAPPFWQDIDR
                     MPPDEAAEATALRQTYASAALNGPFAFLFGYNGGLIGLNDRVKLRPLVVAERGDTVYM
                     ASEESAIRVVSPDLDTLFYPVAGQPVIARLYSETAEKTSVAHGGTVHGD"
     gene            8500..9279
                     /locus_tag="PLT86_08265"
     CDS             8500..9279
                     /locus_tag="PLT86_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36643.1"
                     /translation="MATEPTLAPSVTEPAVISAAGLSYRHLNEQVRSAIRSGATEITL
                     NDVMGHRYIGTGIHAPGVTIHVNGVPGEDLGAFLDGPHIIVHNNAQDGVGNTMNEGRI
                     EIHGQAGDVVGYGMRGGRILIRGDVGYRVGIHMKGYRDKQPLIVTGGCAGNFYGEYMA
                     GGCQVLLGIGQSEKKPVVGQYCASGMHGGVMYIRGELDDWKLGKEVQQIPLDRHDREF
                     LKPVLEDFCQTFAVKEKLLEFDRFVRIAPVSARPYGRLYVY"
     gene            9289..9972
                     /locus_tag="PLT86_08270"
     CDS             9289..9972
                     /locus_tag="PLT86_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT36644.1"
                     /translation="MNFTNSLKALDKLIGSTLRALRESQKLYDAEGIQNEELEKTLKK
                     TGAELRELRKSFSAILAAHAGTFEMVRYLNEGLRMEYQTILDYERYVNVVEDATLATR
                     LRDFGAEERRHAHALSLKITELGGEPKFTVAHERRPDLTAFELLQQHLATEREAVKYY
                     DMGLEKFDDPGFRWLIGKVKVDEEEHLKRLEELIEQYRDTAVLVQESKNFKWIDPYMG
                     KPGDRAWIE"
     gene            10185..10673
                     /locus_tag="PLT86_08275"
     CDS             10185..10673
                     /locus_tag="PLT86_08275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT36645.1"
                     /translation="MPKSYSAPPSMSIDPAVCYHAKIRTSRGDIRIELFAKDAPVTVN
                     NFVFLARDGFYNGLTFHRVITGFVIQGGCPNGDGRGGPGYRFADEVDKNPHRHTAGAL
                     SMANAGPGTNGSQFFITHTPQPHLDGKHTVFGRVIEGMDTVLSVRQGDEIQRVEIVEQ
                     PA"
     gene            10765..>11002
                     /locus_tag="PLT86_08280"
     CDS             10765..>11002
                     /locus_tag="PLT86_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36646.1"
                     /translation="MSAVVSLPGRKTSAISHAAALLLCALAGVPCGCSSNPASPQNKR
                     VRVPTALFHADTSLSVEDAPWSLAIADFSGDSVPD"
BASE COUNT         2246 a         3314 c         3452 g         1990 t
ORIGIN      
        1 aaaacccgcc tgtgcaaaca tacgcacaca ggcggggtag gaaaaaccgt tccgagcgca
       61 acgcgggcgt ggttacctgc tcagcaccat ccggataacc agcgttgtgc tctccgtctg
      121 caggcgggcg aagtagatac cgctggcgag gggaatgccg tttgcatcgc gtccatccca
      181 ggtgacctca tggctgccgg cgctgcggac gccgttcacc agcacacgca tctcctgccc
      241 cagcatgttg tagaccgcca ggttcacgtg cccggtcgcg ccaagagaat accgcaccgt
      301 ggtcgaggga ttgaacgggt tcggtgtgtt tttgagcgta tactggagcg ttgcggcgcc
      361 tttgtcgacc tctgtcggcg cctgcacggc acgttctgct tcctcaccgg cggcaatgag
      421 gtccttcacg acggggtccg cgctccagtc atccaccaga tccgcccgcg ccgtgcgcaa
      481 cgcggaagct gccagtatca acgcgttatt cgccagctcg accgcctcct cgcggttgcc
      541 ctccgccagc gcggccgaca cctgatcgag gagaaccacc acccgttcga gaatcacacg
      601 cacggagtca cttctggccg aatcgttaag gacaaccgcc tgcgccgccc attgccgggc
      661 aagcgccacc agactatcca gcggcaacgt ggttaccggc ggaatcaccg gatcgatgat
      721 gggcgtgcga agcagcgtat ccagcgcgta tcgaaccttc acgagcatct gttccgcttt
      781 cgggtacagg cgggcattga gcgccacttg tgcggagtcc acaagcgccg acgcccgcgt
      841 gacgatagcc cgttcgcgaa ccgcggtcgc gggcaacgat gacagcagcc gcgcgatacg
      901 ggtcctgagc gcggcgattt cccccgctat tcggtccggc gcggtggcga caaagagcgc
      961 gcggtcaagc gccatgttcg aagctccgat caacgcctcc gcccggcgga acgctccctc
     1021 ttccagagcc ttccctgcgc tgtctgcggc ggttttcgcc cgttcaaaga gcgccagagc
     1081 caccgtgtcg ccgctggctt ccacaatcgg accgacgcgt tcaatgcgtt gcgtaagctc
     1141 cccacacatg cgccggagct tcgcctcgag cgcgatcatc tcatgcgcca gccgcaggcg
     1201 ttcccgcgcc cgttgtagca ccgcgtccgc ccggaacacg cgcccctcat cgagaagcag
     1261 gcgcaccgaa tccagcatcg ccggcacggg cgccagcgcc tccgacgcgc gtgcgttggg
     1321 attcagcgaa aggaacagcg ccagcgaatc agcatgttgc cgaaccagat ccaccccggt
     1381 ttcgacacgc gcccgcacgg caagtgcctc gcggatgcgt cccgccagga tacccgcccg
     1441 cgtggtcagg tcgaatgccc gctgttttgc cccctcggag aggtaatccc gtgcgctgtc
     1501 gatcaccgcg tacgcccgcg gaatcatctc cgaagcgggc agattgggct ctgaccggac
     1561 gaccgccacg gcgctgtcga gaaacgcctg gatacggtcc agccgcccgg cgattgccgc
     1621 ggaatccggc aggatgaagg ggggcatgat gatgacgcta tccggcggcg gatacgggag
     1681 ccccgggtcc ggcggcacga tgactatcga actgtcaggc gggaacggcg gggggagggg
     1741 ctggagttgg cccagaagtc gttccagcgt gctgatgatg ccccaaagca tcgcgcgggt
     1801 gggctcatcg tttgcaagag tcccactcgc gaggagattc cggaaaatgg cttctgaacg
     1861 atcaataatc gccaacgcag tggtatcgcc ctgctgcgtt gcgaggccgc gcaggaacac
     1921 gatgagccgt tctgccttct gcaccagttc gcggatgtcc acaggaactg gttcgatggg
     1981 cggaagcggc ggccctggat acgggggaag aatcacgacg ctgtcgccgg gaatcggcac
     2041 ggggggaggg agcggggcaa gtgaactcca cagcacgcgc accttcgcgg cgatttcgga
     2101 gatccgcacc tgtgcgccga ggatatcccc ggatgcgaac atggcctgcg cggtaatcag
     2161 ttcggaagaa agaaggtcaa cctgccggag aagcatgctg tcgccccggg cgctggccgt
     2221 atcccgcaac gcgagaagcc gcgcctcaag ggctgcaacg atatcgggga acgcacgtac
     2281 agcaacggag gagtccggcg gcagaacctg agcccgtacg gggattgcgg aagccgcaat
     2341 cacacccgca aggaaaagcg caaggattcg aggacgcatc ggaagcctcc gcctgcaggg
     2401 cactccagca tgcttgcccg cgtacgcggg cgcatcatgc gtagaccatt actcatgaca
     2461 atgtactttg ctttaaagat aaaagcaatt tgcgttccct cgcgccgaaa cgccgccaga
     2521 aaccagcgcg ttcgagacca ttccggcggg gtgtacggta ccgtacaccc gttggtccat
     2581 ccacgtaccg acgggaacac gtggtccgca gtttttacgg cgatttaacg ttgacgaaac
     2641 tgacctgcaa cgtgcgatgc cgataattag atgagcgtgc gacgcaaaaa caccagacag
     2701 acagcggaga gaaggatgac aggcagtatg aggtccatgt gcgcggttct tgccggcgcg
     2761 gtgctgggtg ccattgtacc ggtggcgggc gcttccccgg taagcatcac tgcagacgtg
     2821 agcggagtaa gccactactt ctggcgcgga tacgatctcc ttgggggcgc gaagcttccc
     2881 gttcaaccgg ccctgacagc aaccgcggag aacgggctat ccgcgggcat ctggggcagt
     2941 gcggcactcc ttgaccgcag caccacggac gccgcagatg aactggactt cacggtgagt
     3001 tactcccggc ccgtggttcc cggcatcgag gcctcggccg gtgggacata ttactatctg
     3061 ccgaacaaca ccattgcctc aaccgccgag ctgttcgcca gcgcagcgtt cgtggagata
     3121 cctttcgagc caaccctgac cgcgtactac gacacgaaat ggctggacga ttccagcgcg
     3181 gaggcggacg ggtggtattt tctggccgcg gcctcccact ccgttccggt gggcagattt
     3241 tccctggacc tcagcgcggg gctcgggttt gggtctaata gcactttcac cggtccgcag
     3301 gacctcaccc tgcgcgccgc gactaccatc cccgtgggcc gtgtgaagct cacgccgttc
     3361 ctgtcaccga ttctggtttt cgaagacgcg gtaaacagcg atactttcgg aatctgcggt
     3421 ggagtaagcg taagctacgc cttctgatga cgactcgcac gcggttttga tttgcgtccc
     3481 gtacgcccgc tggtgcatgt atccacacga cgggcgtccg agatgctgta caccggtgat
     3541 gatcgaaggg cccgcttcgt cgggcctttt gatacgaatg gaacaacggc gtgatcaggt
     3601 tgtgcacagc ggcatttcga cgggccttct gctccgttcg gtggcctccg tgggtatggc
     3661 ggacacgcgg gcggtcccct tccggaaagg gagtagcgca cgaccactgt gccaagtcga
     3721 gaacgcggag gcagcccgcg gcgggccgta cgtgtgtacg cgaatagata gggagattct
     3781 tcaatccgtt cagaatgacc gcttcaccgc gaaacgcccc gatcgggggc cattctgaac
     3841 ggcgagtgcg ggcggggttc cgcaccccgt cagttccgtt tttgtggaat tcgcaccgcg
     3901 caccggcacg cgctcgcaga acgaccggtt ttctgtaaga gatcacctgt tcgggggtca
     3961 ccccgaatca gcggagggca catggaatac tacgaagaag ccaatgcccg cgtggcgctt
     4021 ggtttgcgcc gtcctgaacc gggcgactgg taccacgggt accccgaata ccacgttgaa
     4081 cgcgacgacg acaagtgcat ccgttgcctt gtctgcgtgc gtcagtgtcc atacgactgc
     4141 acgttctacg atccgcatcg ccacggggtg tacaacgttc atgagaactg cgtaaactgc
     4201 cgccggtgcg agttcatgtg cccgacaagc gccatccgga taaccaaatg gggcgccggt
     4261 tttcgtgata acgacaactg gaagcagaag accatccgca cggtgtacaa acaggcggat
     4321 tccggcgcgg taagcctgac ggggctcgga aacgaccgtg agtacgtctc ctatttcgac
     4381 cgcatggtga ttgatgcgtc gcaggttaca aaccccagca ttgatccgct tcgcgagccg
     4441 atggagttgc gcgcgtacat cggaaagaag ccggagcgac acaccaatcc gccggaagtg
     4501 tacccgaacc tgaaggcgga attgccggtt cttttcgccc cgatgtcctt cggagccatt
     4561 gcgttcaact tccagcgtgg ccttgccatg gcggcgaagg aagtaggcac gtatttcaac
     4621 accggagagg gcggcctgca ccccgacctc cgccagtacg gtgaccatgc gatcgtgcag
     4681 atcgcctcgg gccggttcgg agtcagcctc gattatctca gcaacgccgc cgcctgtgag
     4741 atcaagatcg ggcagggagc caagccgggc atcggagggc atctgccggg agaaaaggtg
     4801 acgtgggatg tctcgcgcac gcgcatgatc ccggaaggca gcgatgctct cagccccgcg
     4861 ccgcagcacg acatctactc gattgaagat ctgcgccttc tcatttacgg gctcaaagag
     4921 gccacggaat acacccggcc cgtgagtgtc aagatcgcgg ccgttcacaa cgtcgccgcc
     4981 atcgcgagcg gcatcgtccg cgccggtgct gacatagtgg tggttgacgg ttttcggggc
     5041 ggaaccggag cagctcctac cgtgatccgg gaccacgtcg gcatcccggt tgaggtggcg
     5101 ctggcgcagg ttgaccagcg gcttcgtgag gaaggtattc gccaccaggc ttcgctgatc
     5161 gcggcgggag gaattcggag ctccgcggat gtgatcaaag gcctcgcgct gggtgcggat
     5221 gcgttctaca tcggcaccgc ggcgatggtg gcggtggggt gcaccgcgtg ccagcaatgc
     5281 caccgcgggc gctgctcgtg gggcattacc accaaccgtc cggacctggc tccccgactg
     5341 cagccggagg tagcggcgga gcgattgacg aaccttctcc acggctgggc gcacgagatc
     5401 aaggagatgc tcgggatgat ggggttgaac tccattgaga gcctccgcgg aaatcgcctc
     5461 aaactccggg gcgtcggcat gactcaggtc gaactggaca ctcttggcat ccaacaggca
     5521 gggctctgac gatgaagcgc gtgtacccca gagaagaacg gtgcatcaac tgccatctct
     5581 gcgaagttgc gtgcatcgta gagcattccg agacgaagct ccctgccgcg gcgtaccacg
     5641 tggagggact ccgcttcaat cgcgaggaca gccggacgat ctgcgatccg gcggaagcag
     5701 ggactgcggg tgttcccaag cctctcaacc ggactctggt gcagacagcc gggtacctct
     5761 cgctcagcac caattgccgg cactgcgagg agcccgattg cgtgctcgca tgcaagaacg
     5821 gctccctgta cgtggccgag gatgggcgcg tgctggtgca agaggacaag tgcgtgggtt
     5881 gctggatgtg cgtaatggcg tgccggtacg gcgcaatctc acgcaatccc gcccgctcga
     5941 atgtgcccgc cgttgcgttc aacggcatca accatcactg cgacctttgc cctgaacggg
     6001 aagtccccgc ctgtgtctgg gtgtgcccga caaacgccct cgtttttgaa gacagagacg
     6061 accaatgaac cacgtaatca tcgggaattc gtttgcagct cttttcgcta tcgaggcgat
     6121 caggcgaact gaccaggacg cggagatcac cgtcatcagc cgggaaccgc agcacgtgta
     6181 cagccgggcg atgatccacg aaatgctggc cgggatggtg cgggaaccga tggtgtacct
     6241 gcgcgactgg gatttttaca agcggatgcg cgtaaggacg atgctcgggc ggaccgtaac
     6301 caaaatcaat ccggggttgc agacggtatc taccgaggaa gacgagacgc catacgaccg
     6361 gctgctcatt gccacaggtg ggaaaccgtt tgtgccgccc ctgaatggcc tgcaggacat
     6421 tacgtatcac tcgttcatca ctctcgagga cgccaaaaag ctgaaagccg cgtgtgagaa
     6481 ccggcgcaac gcggtcgttc tgggtgccgg gttgatcggt ctccagtgcg cggaggcgtt
     6541 acggcacatg ggacttaacg tggctgtggt cgaaatggca gataccgtgc tgccgatggc
     6601 gctggacacg gaatccagcc agaccatcgc gaaagagctc gccgacgagg gtatcgccgt
     6661 ccatacgggt gacacgatag acctcattat cagcaacaac ggccacccta cggagtgcaa
     6721 actgcgcagc gggggaacga ttccctgcga tctgctcgta atcgcggtcg gcgtgcgccc
     6781 caatatcgat cttgcaaagg acgccggaat cacaaccgac cgcgggatcg tggtcaacga
     6841 gcacatggaa acgagcgcgg agcacatcta cgccgcgggg gattgcgctc agggaccgga
     6901 gatcgttacc ggcataacga tgccgattcc cgtcattccg gtcgcttcca cgcaggggat
     6961 gattgcgggg tacaacatgg cggggaagcg ccggcggtac ggcggcgcgt tggccctgaa
     7021 tgccatgcag ttcggcggcg ttcaggtgat cagctacggg ttcgtgaaag atgaacgcga
     7081 cggcgaggtg ctcaagtacg tcgacccacc ccggaagatc taccgaaagg tgatcatcaa
     7141 aaacgggcgc attaccggag cgatttttgt caaggacgtt gaccgggccg gcatttttcg
     7201 ccacctgatg gaaaaacgca ttgacgtaac accgttcaag gagcatttgt tgtcgccaca
     7261 tttcggcgta gcgcacctgc ccgccgaaat ccgtgcggac atgttcaccc gcccgcaata
     7321 agaaccagtt gcctgcggat cgaaggagaa cacggggcat gccatcaatg agaggaaacc
     7381 gccacttcgt ccggcggccg catcaggaga aggatatctc cgcatgcggc atcatcggct
     7441 tcatcaatcg cgacggaacg agagtgtccg gtgacgcgat aatccgctcc atcgccaaca
     7501 tgcacgatcg tggcaacggt ttgggcggcg ggtttgcggc gtacgggatc tatcccgaac
     7561 acgccgacgc atatgcgttg cacatcatgt acgacgacga acgcgcgatt gcggacacgg
     7621 aagcgttgat ccggaatacc ttggaaaccg agcatgcgga aacgataccg gtccacccca
     7681 acggcaccgt gacggagtac cctgagttcc gccgatattt ctgccaccct ccggacgtgg
     7741 acgaacgtga tgcggatgag tacatggtgc gcctcgtcat gcggatcaac gcgaaaatcg
     7801 agggcgcgta tgtcgtttcc agcggccgga atatgggggc attcaagggg gtggggttcc
     7861 ccgaggacct ggcggagttc ttctgcctgc aggagtacga agcctacctg tggacggcac
     7921 acaatcgttt tccgacgaac actccgggct ggtggggcgg cgcgcacccg ttcactctgc
     7981 tcgactggtc catcgtacac aacggcgaga tatccagcta cggtatcaac cggcgctatc
     8041 ttgaaagctt cggttatgcc tgcaccctga ggacggatac cgaagtggtg gcgtacatat
     8101 tcgatctcct tgtccgccgg cacggacttt cgttcgaaca ggcggccacg gtgatggcac
     8161 caccgttctg gcaggacatc gaccggatgc cgccggacga agccgcggag gcgacggcgc
     8221 tccgccagac gtacgcctcg gcagcgttga acggcccgtt cgcgtttttg ttcgggtaca
     8281 acggcggcct catcggcctg aacgaccggg tcaaactgcg gccgctggtg gtggccgaac
     8341 ggggagatac cgtctacatg gcgagtgagg agtcggcgat tcgcgtggtg agtccggatc
     8401 tcgacaccct cttttacccc gtcgccggcc agcctgtcat cgcgcggctc tattcggaga
     8461 cggcagaaaa gacatctgtt gcgcacggag gcaccgttca tggcgactga acccacgttg
     8521 gcaccgtccg taaccgaacc ggcggtgatc tccgcagcag gtctttccta ccggcacctt
     8581 aatgaacagg tgcggtcggc gatccgttcg ggcgcgacag aaatcactct caatgacgtg
     8641 atgggtcacc ggtacatcgg caccggcatc catgccccgg gggttactat ccacgtgaac
     8701 ggcgttccgg gcgaagacct tggcgcgttt ctcgacggcc cccacatcat cgtgcacaac
     8761 aacgcacagg atggcgtcgg caatacgatg aacgaaggcc gtattgagat acacggtcag
     8821 gccggcgacg tcgtggggta tggcatgcgg ggcgggcgca ttctcatccg gggcgatgtc
     8881 ggctatcgcg tcggaattca catgaagggt taccgcgata aacagccgct cattgtaacg
     8941 ggtgggtgcg cagggaactt ctacggcgag tacatggcgg gcggttgcca ggtgcttctc
     9001 ggcatcgggc agtcggaaaa aaagccggtt gtcgggcagt actgcgcttc gggtatgcac
     9061 ggcggggtga tgtatattag aggggagctc gacgattgga agctggggaa agaagtgcag
     9121 cagattccgc tggacagaca cgatcgcgag tttctgaagc ccgttcttga ggatttctgc
     9181 caaacattcg cagtcaagga aaaactgctg gaattcgacc ggttcgtcag aatcgcaccg
     9241 gtgtcggcac gtccgtacgg acgcctgtac gtctattgag gagctaccat gaacttcacc
     9301 aactccctga aagcgctgga caaattgatt ggttcgaccc ttcgagcgct cagagagagc
     9361 cagaaactct acgacgcaga gggcatccag aacgaagaac tggagaaaac cctcaagaaa
     9421 accggcgccg agttgcggga gctgcgaaag tcattttccg cgattctggc agcccacgcc
     9481 ggcacgtttg agatggttcg ttacctgaac gaaggtcttc ggatggagta ccagaccatt
     9541 ctggattacg agcggtacgt gaacgtggtg gaagatgcca cgctggcaac ccgtctcaga
     9601 gatttcggtg ctgaggaacg ccgccacgcc cacgcgcttt ccctgaaaat caccgagctg
     9661 ggcggcgaac cgaagtttac cgtcgcccat gagcgacgcc cagatctgac ggcatttgaa
     9721 ctcttgcaac aacaccttgc caccgaacgg gaggccgtga agtactacga catggggctg
     9781 gagaaattcg acgaccccgg attccgctgg ctcatcggca aagtgaaggt tgatgaggag
     9841 gagcacctga aaaggctgga ggagttgata gaacaatacc gcgatacggc ggtactcgtg
     9901 caggaatcga agaacttcaa gtggattgac ccctacatgg gcaaaccggg cgaccgcgcg
     9961 tggattgagt aaccacaggc agggggctcg cccggcctgt gggccacata cctctttacg
    10021 cccgccgtgg tcgtggagat ctcgccttcg gggcgcccat ccgcaccgcc gccgcgcggc
    10081 gcctgtcttc ctacccgcgt cactcggttg gtatctcccc acgcgcagcc gtatttttcc
    10141 gaaagaccct ctcaccagtt tgtcatcccc tcgaaggagc aatgatgccc aagagctact
    10201 ccgctccgcc gtcaatgagt atcgatccgg cggtgtgcta ccacgcaaaa atccgcacgt
    10261 cgcgcggcga catccgcatc gagctgttcg ccaaagacgc gccggtcacg gtcaataatt
    10321 tcgtgtttct tgccagggac gggttctaca acggtctgac gtttcaccgt gtgatcacgg
    10381 gctttgtcat tcagggagga tgccccaacg gcgatggccg cgggggcccg gggtatcgct
    10441 tcgccgacga ggtggacaaa aacccccacc ggcacaccgc cggagccctt tccatggcaa
    10501 atgcgggacc gggcaccaat ggaagccagt tcttcatcac ccataccccg cagccgcatc
    10561 tggacggaaa gcacacggtg ttcgggcggg tgattgaagg catggacaca gtgctttcgg
    10621 tccgccaggg cgatgaaatc cagcgtgtgg agattgtcga acagcctgcg tgaccaggcg
    10681 gcctgtgtta ccaggctgcc tgtcttgcca agcggcctgt gtaaccaggc cgcccgaatc
    10741 acggggatac gcgatgggaa gaggatgagc gcagtggtat ctctgccggg caggaagacc
    10801 agcgcgatca gccatgcagc ggcgttgttg ttgtgcgcgc tggccggggt gccttgtggt
    10861 tgctcgtcga atcccgcgtc gccgcagaac aaacgggtgc gcgttccaac tgcgctgttt
    10921 cacgccgata ccagtctctc ggtggaggac gcgccgtggt cccttgcgat agcagatttc
    10981 agcggggaca gcgtcccgga cg
//
LOCUS       DAOSOO010000099        10720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6142_length_10720_cov_5.982466, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000099 DAOSOO010000000
VERSION     DAOSOO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_10720_cov_5.982466"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLT86_08285"
     CDS             complement(<1..480)
                     /locus_tag="PLT86_08285"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT36647.1"
                     /translation="MNTSSNLRGVLPVFQTPFREDYSIDFDVLRHEIEWLYECGADGT
                     VMAMVSETLRLSTEERRELAAAACKFGCQRGAVVISVGAESTFVAESLARHAVSVGAT
                     AVMAIPPVSVAVGEDELLRYYEAILRGIRIPLIVQDASGYVGKPMSIEIQARLLNEFG
                     "
     gene            complement(506..1591)
                     /locus_tag="PLT86_08290"
     CDS             complement(506..1591)
                     /locus_tag="PLT86_08290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT36648.1"
                     /translation="MENLPTPALVVDGAVLRANIERLAAYARGHRLRVRPHTKTHKSV
                     FVASLQVGAGASGLTAAKAGEAEIMATVCEDILVAFPPVDPERCARIAALATHRTVRV
                     ALDSITAAQRMAQAAAAAQSRVGVLVDVDVGMGRTGVQTPEEALRLAEVIDRDPSLHL
                     DGIMYYPGHVWSPPEEQHEALQKVATKLEEVIRFWTKGGLKADIVSGGSTPTAYQSHL
                     VPQTTEIRPGTYVFNDMNTVHGGFCTIEDCAARMICTVVSTAIPGQFVIDGGTKTFTS
                     DRCIPRPESGHGYIIEYPEARIFALSEEHGQVDARACSRVPKVGERVTVIPNHICPCV
                     NLQDSFWIAEPGNPLRRITVDARGKLS"
     gene            1746..4001
                     /locus_tag="PLT86_08295"
     CDS             1746..4001
                     /locus_tag="PLT86_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOT36649.1"
                     /translation="MSCPNSRHDLLYELPSLEWKTGAPLGNGHLGVMAWGDGSPLCLT
                     LDRDGLWDLRATPPPDDADYNWGTIRRLAKSGSWDELAAIMERNHYYHAPTPAKLPVG
                     RMELWVPGSGVKRGFSARLSLRNATLTAQIGTVRLEVFVAATAPVIAVRLSGGADGIR
                     VRWRSLSDLNAGQAQELGLERHRIVSISGASAAVQRLPDGTTSVVAWKQIRTGSGITL
                     LCTIATSRDGPNPEQIAARILNGVAHSLASMRRQHVNWWRRFWSKSEITIPEKRLETL
                     WFYGLYKLASSSRAGHLPANLQGLWVTDGVLPPWVGEYAANMNVQETYWPAYATNHLE
                     LGTPLYDWVEQIAEGGKERTRRFFGWDGLRLESALAADGTPVPSWGTVQFWPGAATWL
                     AHHFWLHWRYSRDLEFLRRRAYPLFRACMDFWARFLEEDEDGFLHVPLSYSPELGGNA
                     PSAWGRDTVIDLSLIRNLCAWLMESSSVLNLDAGERRAWQQIAARLVPYPKDESGALM
                     VMEGKPLPCSHRHHSHLMPLFPLGDVHVEGTDDDRRCIHASITDLERKGMGEWTGWSF
                     PWASLIASRIGRGEMAANMLRLYLDSFVLPNGLHVNGDWRKNGICIFHYEPFTMEAEC
                     AATAALTEMLVQSWGGKIRLFPAVPASWPDAAFSGLLAEGNVSVSAVRRAGTVEQATF
                     QCPVATEISVVGLDSTAQWTGVRSAIWNHGLWSVALRPATPATARQIARKLPNAFARE
                     PQPRNVFGLRG"
     gene            4069..6339
                     /locus_tag="PLT86_08300"
     CDS             4069..6339
                     /locus_tag="PLT86_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOT36650.1"
                     /translation="MANAGASQPLPRHDLLYERASDEWNTGAPLGNGQLGVMVWGDGN
                     PLRLTLDRDDLWDLRVPSRPDDPSFTWQNLKKLAQEEDWETLNATVERNLNPPAPTPT
                     KLPLGRLEIEFRGTSSNGNGFRGRLSLKKAEFSATFGGNRVKVFVCAHAPVVVVECYG
                     ESASPLVFWRSFRDLNPGQAASLGIQPCDSLQIYDITAAVQRFPDGTTAVVAWHCAPS
                     AGGFVVRCTVATSADGDDPVSVAADRLRAATSTYSLKRRHREWWSDFWSRSSITIPEK
                     RLETLWYYGLYKLASSSRPGNLPANLQGLWVTDGVLPPWRGDYHANMNIQETYWPVYA
                     TNHLEIGLPLLQWIESITEPARQRTRSFFGWDGVRIETALAPNGVPVPGWGTVQYWPG
                     AATWLAHHLWLHWRYSGDRNFLREKAFPFFQLCFAFWEGYLEKGTDGYLHVPLSHSPE
                     WNGNLPEAWGRDPVIDLSLIRNLCLWLDESARELRVSGEMQTRWKVVAESLAPYPRDP
                     AGGLMLMEGVPLSESHRHPSHLMPVFPMHDMSAEGTEEDRKCIEVSLAQLERKGMGEW
                     TGWSFPYVSLIASRVGRACMAEYMLKLYIESFVLPNGCHANGDWRGNGICQYHYSPFT
                     MEAECAATSAVTEMMLQSWGGTIRLFPAVPETWLDASFHGFLAEGGIEVSAVREFGET
                     TEVTLRSRDRAEARVVGLKGNAQWRGAASARWETGTWVVTLDAGVPACGRCHRRKTGK
                     RRATESNTRNIFGLRI"
     gene            6371..7465
                     /locus_tag="PLT86_08305"
     CDS             6371..7465
                     /locus_tag="PLT86_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013129380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT36651.1"
                     /translation="MVSVQLEDVTKTFGSARAVDRVTLNIARGELFFLLGPSGCGKTT
                     LLRMIAGFYEVDEGRILFDGRDMTGVPPHKRNTGMVFQNYALWPHLSVAENVAYGLEV
                     RKVPVTEKQDRVMRALEMVRMDSLAGRSPNQLSGGQQQRVALARALVIEPEAVLLDEP
                     LSNLDAKLRLEMREEIRRIHDELGLTMIYVTHDQKEALSMADRLAVMNRGRIEQTGAP
                     RQVYVTPENVFVAGFIGETNLFPGTLLPDGSSGAELQVDTPYGVLRVARKSSTAEPGK
                     VSVSIRPEAVSVRGAEEALPGGILRGVVQSIIYLGDTEQYVVRLADGKLVRSVEANPA
                     RPRARTGEEVHLTFRSEDAIALPPDTSNGG"
     gene            7467..8882
                     /locus_tag="PLT86_08310"
     CDS             7467..8882
                     /locus_tag="PLT86_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT36652.1"
                     /translation="MSDTGKVTYIMRFGGTRARRFWSTTLTWVLFCVPAAVVAGCSAA
                     GRADDELVILSPHWEGIQQEFERGFAEYWKAKTGRSVDITWLDQGGTSQILRFISSEY
                     KNSPEGIGIDLMFGGGVDPYLKLVKDGVTHPFTLPDSVLKRIPAHFAGMPMYDSTGRW
                     YGATMSGFGIVFNKAVARRLGRFAPHTWKEMGDPAWRGWIGAGDPRLSGSIHMAYELM
                     LQAYGWEEGWKTITAIAANTRSFARGAAEIPLSVARGDAVAGMCIDFYAWSEVTHVGS
                     SELSFVYPVNLTAVNPDAICILRGAPHLNLAKEFVTFVMSEPGQRLWSFKKGAPGGPL
                     KNQLNRFTVMPDLYERYPEHIAVDENPFSWRITFQYDSGLGSRRWSVVNDLIGAQLID
                     SHRELEQAWEALRQRGMPEEGLSLLASQPVSPEEALALAEQWKDPAIRNVYLSQWTAF
                     ARDKYRAVIRGEYGGNRQYTQ"
     gene            8892..10580
                     /locus_tag="PLT86_08315"
     CDS             8892..10580
                     /locus_tag="PLT86_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT36653.1"
                     /translation="MGSKTLTPASAVFLVLLFGVFAVFLVYPVGYVLESAFYREGVFT
                     LEFFRLMFTNPVHREAIINSTMLAITVAAVTTVIALPISFVMVRRDFPGKPLLNGLLL
                     VPMVMPPFVGAVGMQQLLAKFGSVNLLLIRIGLMSPRDPFDWLGTGFWAVVLLSVLHL
                     YPIMYLNVAAALANVDPSLEEAGRNMGASGFRLFRSVTFPLMLPGYFAGAIIVFIWAF
                     TDLGTPLIFNYSRVVPVRIFEAVSDIHENPMGFALVVLVLAMTVVFFAISKKVLGSSN
                     YQMMARGHVQGSSSPLPRRWVPVVWVSIAAVIGCALVPHASVVLSAIADTWFMTILPS
                     SYTTVYIGRVFTHPLTLSSIRNSLFLSSLSTLVDVALGVTIAYFLARKRFIGRDVLDG
                     LAMLPLALPGLVLAFGYVAAFGGSFLDIKRNPMPLLIIAYAIRRLPYMVRAAYAGLQQ
                     TSETLEEASLNLGAGPLRTLRRITLPLVFANIVAGSVLAFSFAMLEVSDSLILAAKEE
                     FYPITKAIYAMSLRVADGPYVASAMGLLGMVLLATSLIVAGRFLGKRLGELFRA"
BASE COUNT         2097 a         3035 c         3283 g         2305 t
ORIGIN      
        1 tccgaattcg ttgaggaggc gagcctgaat ctcgattgac attggcttgc ccacgtaacc
       61 gctggcgtcc tgaacgatca gaggaatccg tatcccgcgg agtatggctt cgtaataccg
      121 taacagctca tcctcaccca ccgcgacaga tacgggagga atcgccataa ccgcagtggc
      181 tcccacactt accgcgtgtc gggcaagact ctctgcaaca aatgtggatt cagccccaac
      241 gctgataacc accgctccgc gctggcaccc gaatttgcag gcagccgccg caagctcacg
      301 ccgctcttct gtggaaaggc ggagtgtttc ggagaccatc gccatgacgg tgccgtcggc
      361 accgcattcg taaagccact ctatctcatg gcgcaatacg tcgaaatcaa tcgagtaatc
      421 ttctcgaaac ggcgtctgga atacgggaag cacgccgcgc agattgctgg aggtgttcac
      481 ggttgccttt cgtcgacaaa aaggttcagg atagctttcc tctggcatca acggtaattc
      541 tgcggagtgg gttcccgggt tccgcgatcc agaaagaatc ctgcaaattc acgcaggggc
      601 agatgtgatt cggaatgacg gtcacgcgct caccaacctt tggcacgcga gaacacgcgc
      661 gtgcatctac ctgcccgtgc tcctcgctca gggcaaagat gcgtgcttcc ggatactcga
      721 ttatgtaccc gtgcccgctt tcgggtctcg gaatgcaccg gtcactcgtg aacgtttttg
      781 ttccgccgtc aataacaaat tgcccaggaa tcgccgtgct gaccactgtg cagatcattc
      841 gcgcagcgca gtcctcgatc gtacagaatc caccgtgaac cgtattcata tcgttgaaga
      901 cgtacgttcc cggacgaatc tcggtggtct gcgggaccag atgggactgg tacgcggtag
      961 gcgtggagcc cccagacacg atgtcagcct tcaatccgcc ctttgtccag aacctgatga
     1021 cctcttccag tttcgtcgcg actttctgca gcgcctcatg ctgttcctca ggagggctcc
     1081 acacgtgccc ggggtagtac atgatcccgt ccagatggag cgagggatcg cggtcgatca
     1141 cttcagcgag acggagagcc tcttccggtg tctggacacc cgtcctgccc atgccaacgt
     1201 ccacatccac gagtactccc actcgcgatt gcgcggcggc agcagcctgt gccattcgct
     1261 gggccgcggt gatcgagtcc aacgcaacgc gaacggttct gtgagtggcc agtgcggcga
     1321 ttcgggcgca tcgctccggg tccaccggcg ggaacgctac gagtatatcc tcgcagaccg
     1381 ttgccatgat ttccgcctcg cccgctttgg cggcggtcaa gccacttgcg cccgcgccta
     1441 cctggagcga tgcgacgaaa actgatttgt gtgtcttcgt gtgagggcgg actcggaggc
     1501 ggtgtccacg cgcgtaagca gcaagacgtt cgatattcgc gcgcagaacc gcgccgtcaa
     1561 ccacgagtgc gggagtaggc aggttttcca tgggtatgaa taaccgagcg tgagggcgtg
     1621 cgagtcaatc cgtatcggcg caacggtatt cccgttgggc atgggtgggc tttgccgtac
     1681 gttaagttaa cgtctgacca attcgagaaa acagcccatc agcctttcga gatagagccc
     1741 ttccgatgtc ctgtccgaat tcgcgccacg acctgctcta cgaactcccg tcattggagt
     1801 ggaagacggg cgctcctctg gggaatggcc atctgggtgt catggcgtgg ggtgatgggt
     1861 ctcctctgtg tctgactctt gatcgtgacg gtctctggga tctccgggcg acgccgccac
     1921 ccgacgatgc ggactataac tggggtacca tcaggcgtct cgcgaagtcc ggctcatggg
     1981 acgagttggc ggcgatcatg gaacggaatc attactacca cgcgcctacc cctgccaagc
     2041 ttccggtcgg gaggatggag ttgtgggtgc cgggcagcgg cgtaaaacgc ggtttttcgg
     2101 cccggttatc tctcaggaat gccacgctca ccgcgcagat cggcaccgtt cgcctggaag
     2161 tgtttgttgc ggcaaccgcg cctgtcatcg ccgtgcggct cagcggggga gccgacggta
     2221 tccgtgttcg ctggcgatca ctcagcgatc tgaacgccgg gcaagcgcag gagcttggcc
     2281 tcgagcgaca caggattgtc tccatctcgg gcgcaagtgc cgcagtccaa aggttacctg
     2341 acggcacaac ctcagtggtt gcctggaaac agattcgcac cggttctggc attacgttgt
     2401 tgtgcacgat cgcgacatcg cgtgatggtc cgaaccctga acaaatcgcg gcgagaatac
     2461 tcaacggcgt tgcccattcg ctcgccagta tgcgcaggca gcatgtgaac tggtggcggc
     2521 ggttctggag caaatcggag atcaccattc ccgagaaacg gctggaaacg ctctggttct
     2581 acggcttgta caagcttgca tcctcctcgc gagcggggca tcttccggcg aacctgcagg
     2641 gcctgtgggt gaccgacggc gttctgccac cgtgggtcgg tgaatacgcc gcaaacatga
     2701 acgtgcagga aacctactgg ccggcatatg caacgaacca ccttgagctg ggcacgcctc
     2761 tttacgactg ggtggaacaa atagcggagg gagggaagga gcgcaccagg aggttcttcg
     2821 gctgggacgg ccttcgtctg gagagcgcac tggcagcaga cggtacgccg gtgccatcgt
     2881 gggggaccgt ccagttctgg cccggagcgg ccacctggct ggcacaccat ttctggctcc
     2941 actggcgtta ttcacgggat ctggagttcc tccggcggcg cgcgtatccc ctgttccggg
     3001 catgcatgga tttctgggcg cgattcctgg aagaggatga ggacggcttc ctgcacgtgc
     3061 cgctttccta ctccccggag ttgggaggga acgcgccttc cgcgtggggc agggacacgg
     3121 tgatagacct ttctctcatc aggaatctgt gtgcgtggct tatggagagc tcctctgtgc
     3181 tgaatctcga cgccggggag aggcgtgcct ggcagcaaat cgccgcccgg ctggtgccgt
     3241 atccaaagga tgaatccggc gccctgatgg ttatggaggg gaagccgctc ccttgcagcc
     3301 accggcacca ttcgcatctc atgcctttgt tcccgctggg cgatgtgcac gttgagggta
     3361 ccgatgacga ccgccggtgt atccacgcat cgataacgga tctggaacga aaagggatgg
     3421 gcgaatggac cggctggtct tttccgtggg catccttgat cgcaagccgc attggcaggg
     3481 gcgaaatggc tgccaacatg ctccgcctat atctcgattc ctttgtgctg ccgaatggcc
     3541 tccacgtgaa cggtgactgg cgcaagaacg gaatctgtat cttccactat gagccattca
     3601 cgatggaagc ggaatgcgcc gcaacggcgg ctctcacgga gatgcttgtg caatcgtggg
     3661 gcggcaagat tcggctgttt cccgcggtgc ccgcgtcgtg gcccgatgct gcattctccg
     3721 ggctcctcgc cgagggcaac gtgtctgtca gtgcggtacg cagggcaggc actgtcgaac
     3781 aggcgacgtt ccagtgtccg gttgccacgg aaatcagtgt cgtcgggttg gattcaacgg
     3841 cgcagtggac tggcgtgaga agcgcgatct ggaaccacgg actctggtcg gtcgctctgc
     3901 gacctgcgac tcccgcgacc gcacggcaga ttgcgcggaa attgccaaac gcctttgcca
     3961 gagaacctca gccgcgaaat gtcttcggcc tgcggggtta ggagtggcgc gcggccgtct
     4021 gttcaaggag tgaaggcttc tcacatacat acaacttgcg gggatttcat ggcaaacgcg
     4081 ggtgcttctc aacctttacc cagacatgat ctgctctacg aacgagcttc agacgagtgg
     4141 aataccggtg ctcctctcgg taacggccaa ctcggtgtaa tggtctgggg ggatggaaat
     4201 cctctccgcc tcactctcga tcgcgacgat ctttgggatc tgcgcgtgcc ttccaggcct
     4261 gacgatccgt cgttcacgtg gcagaacctg aagaagctcg cgcaggaaga ggattgggaa
     4321 actctgaatg cgacggtgga aaggaacctg aatcctccgg ctccaactcc gaccaagttg
     4381 cctctgggaa ggctcgaaat tgagtttcgg ggaaccagtt cgaatggcaa cggtttccgt
     4441 ggcaggctct ctctgaaaaa ggcagagttc agtgcgacct tcggtgggaa cagagtgaag
     4501 gtttttgtgt gtgcccacgc gccggtggtg gtggtcgaat gttacggtga gtcggcatcc
     4561 cctcttgtgt tctggcggtc gttccgggat ctcaatccgg gccaggcggc ctcccttgga
     4621 atccagccct gcgactcgct tcagatatat gatattactg ccgccgtaca acgtttcccg
     4681 gacggcacaa cggcagtcgt tgcatggcat tgtgcgccat ccgcaggagg atttgtagta
     4741 cggtgcaccg ttgcaaccag tgctgacggc gatgatcccg tctctgtcgc tgcagacagg
     4801 ctgcgcgcgg ctacaagtac gtattctctc aagcgtcgcc accgggagtg gtggagtgat
     4861 ttctggtctc gttccagcat tacgattccg gaaaagaggc tcgaaacgct ctggtattac
     4921 gggctttaca aactcgcgtc atcatcccgc ccgggaaacc tgccggccaa tctccaaggt
     4981 ctctgggtaa ccgacggagt cctgccgccc tggcgcggcg attaccacgc caacatgaac
     5041 attcaggaaa cgtactggcc ggtgtatgcc acaaatcacc tggaaatagg cctccctttg
     5101 ctccagtgga ttgaaagtat caccgaacca gcgcgccaaa gaacacgaag tttcttcggg
     5161 tgggatggcg ttcggataga aacggccctc gcacccaacg gggtgccggt tccgggctgg
     5221 ggaactgtcc agtactggcc gggagcagca acatggcttg cacaccacct gtggcttcac
     5281 tggcgatact cgggagaccg gaacttcctt cgtgaaaagg cgttcccctt tttccagttg
     5341 tgcttcgcat tctgggaagg gtatctggag aaggggaccg acgggtatct gcatgttccg
     5401 ctgtctcact cgccggagtg gaacggcaac ctgccggagg catggggtcg agatccggtg
     5461 atcgatcttt ccctcatccg caatctctgt ctctggttgg atgagagcgc gcgcgaactg
     5521 cgcgtgagtg gcgagatgca aacccgctgg aaggttgtcg cggagtccct ggcaccgtat
     5581 ccacgcgatc cggcaggcgg tttgatgctg atggagggcg tgccgctctc cgagagccac
     5641 cgtcaccctt cgcatctaat gcctgtgttt cccatgcacg atatgtcagc ggaaggtacc
     5701 gaggaagaca ggaaatgtat cgaggtttca ctggcacaac tggagcgaaa gggcatgggg
     5761 gaatggaccg gctggtcatt cccctacgtc tccctcattg cgagccgggt cgggcgagcc
     5821 tgcatggcgg agtacatgct gaagctgtac atcgaatcgt tcgtattgcc gaacgggtgc
     5881 cacgcaaacg gtgattggcg cggaaacgga atctgtcagt atcactactc accgttcacc
     5941 atggaagcgg aatgcgccgc aacatcggcg gtcaccgaaa tgatgcttca atcatggggc
     6001 gggacgatca gattgtttcc agcggtcccc gaaacctggc ttgacgcgtc atttcatggg
     6061 tttctcgcag agggtggaat cgaagtaagt gcggttcggg agttcgggga gactacggag
     6121 gtaacacttc gttcacggga tcgcgcggag gcgagggttg tgggtctgaa gggaaatgcc
     6181 caatggcggg gagctgcctc agcacgctgg gaaaccggaa cgtgggttgt tacgctggat
     6241 gcgggcgttc ctgcgtgcgg gcggtgccac cgccggaaaa cgggcaagcg aagggcgacc
     6301 gaatcgaata cgcggaacat atttggcctt cgaatctgag aatgacacgg ttttcacgcg
     6361 gagtcaaagg atggtaagtg ttcaacttga agatgtgacg aaaactttcg ggagcgcccg
     6421 cgccgtcgat cgggtgaccc tgaatattgc ccgcggcgag ttgttttttc tccttggacc
     6481 ttcgggatgc gggaaaacga cgcttctacg gatgatcgca ggtttctacg aagtggacga
     6541 ggggcgcatt ctgttcgacg gtcgagacat gacaggcgtg cctcctcaca agcggaacac
     6601 gggtatggtg ttccagaatt acgcgctgtg gccacatctc tcggtggcgg agaacgtggc
     6661 gtacggactc gaggttcgga aggtacccgt aacggagaaa caagatcggg tcatgagggc
     6721 gctggaaatg gtgaggatgg attccctcgc tggccgctcg ccgaatcaat tgtccggagg
     6781 gcaacagcag cgcgtggctc tcgcgagggc cctggtaatt gaacccgaag cagtgcttct
     6841 ggacgaaccg ctctccaatc tggatgccaa actcaggctc gaaatgcgcg aggagattcg
     6901 cagaatacac gacgagttgg gcttgactat gatttatgtc acgcacgacc agaaagaagc
     6961 gttgtccatg gctgatcgac tcgccgtgat gaatcgcgga cgaatcgagc aaacgggagc
     7021 gccacgtcag gtgtacgtga cacctgaaaa cgtctttgtc gcgggcttca tcggggaaac
     7081 caatcttttc cccggcacgc tcttgcccga cgggtcctca ggtgcagaac tgcaagtgga
     7141 cacgccttac ggcgtccttc gggtggcgag aaagagcagt actgcggaac cgggcaaagt
     7201 gtcagtatcg atccgtccgg aggcggtaag tgtccgaggg gcggaggaag ccttgccagg
     7261 gggaattctg cgcggcgttg tccaatccat catatacctt ggcgacacag agcagtacgt
     7321 cgtgcgcttg gccgatggca aactcgttcg cagcgtggag gctaatcccg cgcggccgcg
     7381 cgcccgcacc ggtgaggagg ttcacctgac gttccgttcc gaggacgcga ttgcgttgcc
     7441 gccggacacg agcaatggag gctgaggtgt cggacacagg caaggtcacg tacatcatgc
     7501 ggttcggcgg aacgcgtgcc cgcaggttct ggagcacgac gctgacgtgg gtgctcttct
     7561 gcgtgccagc cgcagtagtg gccgggtgtt cggcggcggg acgggcggac gacgaacttg
     7621 tgattctttc tcctcattgg gaaggtatcc agcaggaatt tgaacgagga ttcgcagagt
     7681 actggaaagc gaaaaccggt cgatccgtgg acattacatg gcttgatcaa gggggaacat
     7741 cgcagattct tcggttcatc agttccgaat acaagaactc gccggaagga atcggtatcg
     7801 atctcatgtt tgggggcggc gtcgacccgt acctcaaact tgtcaaggac ggcgtaacac
     7861 atccattcac gcttcccgat tcagtgctga aaagaattcc agcccatttt gccggcatgc
     7921 cgatgtacga ctccactggc aggtggtacg gcgcgaccat gtccgggttc ggaattgtgt
     7981 tcaacaaagc tgttgccaga cgactcggaa ggttcgcgcc tcacacgtgg aaggaaatgg
     8041 gcgatccggc atggcgcggc tggattggag cgggtgatcc caggctctcg ggaagcatcc
     8101 acatggcgta cgaactgatg ctccaggctt acgggtggga ggaaggctgg aagaccatca
     8161 ccgcgatagc cgccaacacc cgatcgttcg cgcgcggcgc cgctgaaata cctctctccg
     8221 tggcgcgcgg cgacgctgtc gccgggatgt gcatcgattt ctacgcgtgg tccgaagtta
     8281 cacacgttgg ttcgagtgag ttgagtttcg tctatccggt caaccttacg gcggtcaacc
     8341 ccgatgccat ctgcattctc cgcggtgcac cacatctgaa tctggcgaag gagttcgtga
     8401 cattcgtgat gagcgaacct ggccaacgcc tgtggtcgtt caaaaaaggc gcgccgggcg
     8461 gtcctttgaa aaaccagctc aatcgcttca ccgtaatgcc ggacctgtat gagcgctatc
     8521 ccgaacacat cgccgtggac gagaaccctt tctcgtggcg aataaccttt cagtacgact
     8581 ctggcctcgg ttcgcgacga tggtcagtgg tgaatgacct tatcggcgcg cagctgatcg
     8641 actctcaccg tgagctggaa caggcgtggg aagcgctccg ccagcgcggt atgcccgagg
     8701 aagggctgtc tcttcttgcc tcgcaaccag tgtcgccgga agaggcgctt gcgttggcgg
     8761 aacaatggaa agaccccgcc attcgaaatg tgtatctctc gcaatggacg gcatttgcgc
     8821 gggataaata ccgggcggtg atccgcgggg aatacggcgg caacagacag tatacacagt
     8881 aggatctcga catgggcagt aaaaccttga cccccgcatc tgcagtcttc ctggtgctcc
     8941 ttttcggcgt gttcgccgtg tttctcgtat accccgtggg atacgttctg gagagtgcct
     9001 tctaccgtga gggtgtgttc acgcttgagt tcttcaggct gatgttcacc aacccggttc
     9061 accgggaggc catcatcaac agcacgatgc tggccataac cgttgccgcc gtgacaaccg
     9121 tcatcgcgct gccgatttca ttcgtgatgg tgagaaggga tttccccggg aagccgctgc
     9181 tgaacggcct tttgcttgtt cccatggtga tgccgccgtt cgtcggcgca gtcggcatgc
     9241 agcagcttct tgccaaattc ggttccgtca acctgctctt gatcagaatc ggccttatgt
     9301 ctccgcgaga cccgttcgac tggctcggta cgggtttctg ggcggtagtg ttgctgagcg
     9361 ttctgcacct ctatccgatc atgtacctta acgtggcggc ggctctcgcc aacgtcgatc
     9421 cgagtctcga agaagcgggc agaaacatgg gggcttcggg tttcaggctg tttcgttccg
     9481 ttaccttccc tcttatgctg ccgggttact tcgccggcgc gatcatcgtg ttcatctggg
     9541 cgttcacgga cctcggtact cccctcattt tcaactactc ccgcgtagta ccggtaagaa
     9601 tcttcgaggc agtgagcgac atccacgaaa acccgatggg cttcgcgctc gttgttctcg
     9661 ttctcgcgat gactgtcgta ttcttcgcga taagcaagaa ggtgcttgga tcgagcaatt
     9721 accagatgat ggctcggggc cacgtccagg ggtcgtcttc cccgcttccc cggcgatggg
     9781 tgcctgtggt atgggtctcg atcgcagccg tcatcggatg tgcccttgtt ccccacgcaa
     9841 gcgtggttct ttccgccata gccgatacgt ggttcatgac cattcttcct tccagttaca
     9901 ccaccgtgta catcgggcgc gtattcaccc atccccttac tctgtcgagt attaggaaca
     9961 gcctgtttct cagttcgctc agcactctgg tggatgtggc cctcggcgtg acgattgcct
    10021 atttcctcgc gaggaagcgc ttcatcggcc gggacgtttt ggacgggctg gccatgttgc
    10081 cactcgcgtt gcccgggctg gtgctcgcct tcggctatgt cgctgcgttt ggtggctcgt
    10141 ttctggacat caaacgaaac cccatgcctc ttctcatcat cgcgtacgca attcggcgac
    10201 ttccttacat ggtgcgggct gcgtacgccg gacttcagca aacgagcgaa actctggaag
    10261 aggcgtcgct gaacctcggt gcgggaccgc tccgaacgtt gagacgaatc acgctcccgc
    10321 tcgtgttcgc aaacatcgtt gccgggtcgg tgctcgcctt ctcgttcgcc atgctggagg
    10381 tctcagacag cctgattctc gcggcgaaag aggagttcta tccgattaca aaggccatct
    10441 acgcgatgag tcttcgtgtt gcggatgggc catacgttgc cagcgcgatg ggattgctcg
    10501 gaatggtgct cctcgccacg agtctgattg tcgccggaag attcctcggt aaacgccttg
    10561 gcgaactctt cagggcctga tcttgcggcg ccgggagtgg aagccgtaag caagcctctg
    10621 tctgccgttg gcatcggggt gtggtccgtt tccttccgcg gaacatgtcc acggggaata
    10681 cggcaggcac cggcagccct cgtgcgagaa aactcgccgc
//
LOCUS       DAOSOO010000100        10676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6185_length_10676_cov_5.628001, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000100 DAOSOO010000000
VERSION     DAOSOO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_10676_cov_5.628001"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1105)
                     /locus_tag="PLT86_08320"
     CDS             complement(53..1105)
                     /locus_tag="PLT86_08320"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT36654.1"
                     /translation="MKHVVVYHEVEKFAAWPANNGLWSWEGKEALVGFTVGGYEEKKG
                     HNINEPYHSFLAKSTDQGETWRAYEPENFVGSDAPSVPLEAPLDFTEPGLALRFAGVG
                     YHGGKRPEGAVFVSRDRGDTWQGPFTLGGIHRHPELHGGEITARTDYVVLGPHDLIVM
                     MSARVGDSKTNRTFCARTTDGGLTFRFMSWVVSPSESARSIMPSTVRCASGKLVTAVR
                     RIAAESNSCWIDAFASVDTGRWWTKIAQVNNPVGWSGNPPSLVALRDGRLCCVYGDRD
                     RACIVAKYSPDEGRTWQGETILRDDYHRDSFDDPDLGYPRAFQRADGLLTVAYYWATK
                     ERPHHHIAGTIWDPRV"
     gene            complement(1115..2239)
                     /locus_tag="PLT86_08325"
     CDS             complement(1115..2239)
                     /locus_tag="PLT86_08325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT36655.1"
                     /translation="MKHVIVYEHPDAFVGWPANNGLWSWDGKEVLVGFTVGGFEEKPG
                     HNITEPYRSMLARSTNQGDTWKAFQPANFVRPGITATALTEPLDFTAPGFAMRVVGIG
                     YHGSDEPRGMFFASNDRGATWQGPYSFGDLMQHEELAGLSFTSRTDYVVEGPREMLVL
                     MSARKEGGGMVDRVFCARTTDGGLTFRFVSWMVPPSDPYRAVMPATVSFAASRGSATV
                     SFAASRGSATVSFASGRLVAAVRRREIGADRSCWIDAYASTDGGATWQWQVKIGDTGG
                     QNGNPPALTKLRDGRLCCVFGNRDQAVMLAKFSEDEGRTWRQETVLRDDFQHDKFNDA
                     DMGYPRVFQRADGKLMAMYYWATKDHFHHHIAGTIWDPED"
     gene            complement(2272..3165)
                     /locus_tag="PLT86_08330"
     CDS             complement(2272..3165)
                     /locus_tag="PLT86_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36656.1"
                     /translation="MSLPPRSEKSPRRWDLVTPQAAFSIRDTAEPFVFGDAMWLSNGY
                     YYGGVLSRDLWRSTDGAEWTMVSDATPYDGYSEMVVHDGRVWAVKGSVWTSADGVTWN
                     QVLDKTPFGVRGYGEVVVLNGCMWQLGSGNDVWCSRDGEKWECVCVDAPFGPRYGSAV
                     AVHDGALWLMGGATQEKGDPPEKHYPDFVTHNDVWRSVDGADWECVTAHAPWSERQWV
                     VAEVYAGRLWTIGGFSNRESCNLGDVWYTENGTTWHEYVPKPGFSPRHEATPYVFDGS
                     LWVVAGNSWPLMNDVWRLTLG"
     gene            complement(3162..3494)
                     /locus_tag="PLT86_08335"
     CDS             complement(3162..3494)
                     /locus_tag="PLT86_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36657.1"
                     /translation="MVVGFGAFSIGVMGVSELQGAEASLACVIAICGAFVSIGYRTRW
                     AAAVLLLIWLAYSFFDRELWPFGIIWLAVGIVLLAGAPSFSVDARLAARREGRTVPDP
                     SDISGTLE"
     gene            complement(3675..5024)
                     /locus_tag="PLT86_08340"
     CDS             complement(3675..5024)
                     /locus_tag="PLT86_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36658.1"
                     /translation="MTLEESRNLPTAREVDVLIVGGGPAGVGAGLAAARMGADTLVVE
                     QFNCLGGVATAGGHGHISKYDEEGTGRRIVGGIADEIASRVVSGGFGVRNSHGIMFEV
                     EGMKLLLEAMAEETGVGLLYHTFFCDTVVDDGAAIGAVIQNKDGRQVIRARRIVDCTG
                     DGDVAFRAGCPYEVGRPSDGKCQPVTLMFTIGGVDWPTVEKFRVDYQLKEVWERAQRE
                     GNMRPFQKTIMGWWWTPTRPDQVGVNFTHVTHIDSTKAADLTRATIEARKQAYETIEV
                     YRKYVPGMEHCYMISTPNTVGIRESRRIMGDYVLTEDDVKGQREFSDAICYGSFFVDI
                     HCIDGPGMDSTSWRPSRGFKYQIPYRILVPRGAENLLTAGRCVSCTHLALGSIRVMVP
                     CIAMGEAAGTASALSLRDGVAPRAVDVTALQAQLRQQGAILYEEDIVTVERNSQMAR"
     gene            complement(5054..6586)
                     /locus_tag="PLT86_08345"
     CDS             complement(5054..6586)
                     /locus_tag="PLT86_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36659.1"
                     /translation="MGNEGVKGFDHVGFYLHASWVFNYPLAVRTWAPADYDAMFRLLG
                     GLGMDRVMIWPMSEIAPPPLSESDAQHLRGFRDIIATARQRGLECWLTFCPNLSTREE
                     IRGTPVKDRVFYPFMRTFRLDDEHQFHEYIAHMRSLLACMNNADGYVFIDGDPGGYPG
                     AHPRDYFRMLGAVRAILDDVAATRPKLIPWIWCGWGADWAGEGAWKPDLRKLVRPFLE
                     ELKRNPPAEPWELLPGRSIRDGWGNGRINFELVEEAGLVERSTLMTYEIIEFEPTPPA
                     FVIQFDDIRRVVGEELRYAGAAKGIFGNAQQPVSALHNLIYFARCAKDPSWLTKSDDE
                     VLHAIAQLLGGDAAVLVPAWKSATARLEELPADLSERVRGSRLKSDLAACIPGGPETY
                     LGLLGDFVEARRTVLSNTAVLPRTRAEAIVRLRGAVAGIIAWWLRHRYVFSGEGGPHF
                     DWNHAHHASFIAPLHTWIRDGGVTADTELQKALADEITGMTGMPRETAESLIARLFAG
                     VR"
     gene            complement(6628..7758)
                     /locus_tag="PLT86_08350"
     CDS             complement(6628..7758)
                     /locus_tag="PLT86_08350"
                     /inference="COORDINATES: protein motif:HMM:NF037031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HOT36660.1"
                     /translation="MSVTVDCDFPGGNIVVDAMDGDTVRLHQDLRDTTTDWFYWYFRV
                     RGAAGRTLTFQFTRSRALGARGAAMSPDGGRNWRWLGAVPVNDNRFSYTFSADADEVR
                     FSFAMPYLEADWRAFAARMARNPGFSTGVLCRTRKGREAEYAVVDATGGKPAHRVAIT
                     CRHHCCEMMVNYTLERLIQWVLESDEDTAEWLRANAAFFIVPFVDKDGSEDGDQGKNR
                     FPRDHGRDYDGRSVHVETAAIRAELPAWADGKLHLALDLHCPWIAGPHNEVIYLVGTE
                     NPRMEAEQRRFSAILESENKGMLPFRASDFLPFGTDWNSSANYTGGKGFTRWAAELPG
                     VRLATAIEIPYANVAGAEVNAESARSLGPDVGAAIARYLQGA"
     gene            complement(8381..9166)
                     /locus_tag="PLT86_08355"
     CDS             complement(8381..9166)
                     /locus_tag="PLT86_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36661.1"
                     /translation="MADDYATQAKGIRILPGMWRPHYPWEQIAWVSPPWPSQDYVWLD
                     FPEAIFTDSGLLFLSHINPPFPVVFPNPPKVPWTQTDTGVSFERVLPNGIAFGGSVER
                     WDGVGARLNLFIRNGSDAPLTRIKLQTCAYLRGIKEFAEFTQANKLVHVPGRGWMPFN
                     EAYATGVETGHYSLGWRSGPKTADLPVMATVSGETGRVAAMTWFESTNALVGNPGHPC
                     MHADPVFPDLAPGARADIRGALMFFDGPVDAFGEWFVREGMKG"
     gene            complement(9185..10507)
                     /locus_tag="PLT86_08360"
     CDS             complement(9185..10507)
                     /locus_tag="PLT86_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT36662.1"
                     /translation="MTQNPLNLVVVICHDLGQHLGCYGVPGIRSPHIDAFADFGVRFA
                     KSFCTAPQCSPSRAALWTGRFPHANGVVGLTHAGFANDLNPDEVHLAQMLRAAGYDTH
                     LFGLQHEARLPERCGHEHIHGTGTCAKVADDFITFMRSRDDAGRPFFAQIGFAEPHRP
                     FPHDDVDALPPDSVSVPPYMPNIPEVREDLSEMEASIASADRAFGRIVEAVRTSPAAD
                     NTILIFTTDHGIPFVHAKMTLYDPGIEVALIMSVPGAAKGIVRNEMISNVDVMPTLLD
                     LLGMPIPTNLHGRSFSALFSDVEYTPNDAVFAEKTYHTYYDPMRCIRTDRWKLIANFE
                     NAPWQETSPDYFNNAKSYVEVSKALNVPYSVQYHVPYELFDLEADPYEQYNLADSPDH
                     RAIRDALIVRLRNWMRDTGDPLLDGPMAQAAYRIRMADFTQAGNQPDG"
BASE COUNT         1909 a         3597 c         3245 g         1925 t
ORIGIN      
        1 atccgctgaa tccgctcgct ggcgtacggc cttcgcagca tcgcggagta cctcagaccc
       61 gcggatccca gatcgtgccg gcgatgtggt ggtgcgggcg ctccttcgtt gcccagtagt
      121 aagccactgt aagcagcccg tccgcccgct ggaacgctct cggatagccg agatcgggat
      181 cgtcgaagct gtcccggtgg tagtcgtcgc gcaggatggt ttcgccctgc catgtccgcc
      241 cttcgtccgg gctgtacttc gccacgatgc atgcccggtc acgatctcca tacacgcagc
      301 agagccgacc atcacgcaac gcaaccagcg acggcgggtt gccgctccac ccgacggggt
      361 tgttgacctg cgcgatcttc gtccaccacc ggcccgtgtc caccgaggca aatgcgtcga
      421 tccagcagga gttggactct gcggcgatcc ggcgcaccgc ggtcaccagc ttgccggacg
      481 cgcaccgcac ggtggacggc atgatcgagc gggcggattc cgagggtgat accacccagg
      541 acatgaaccg gaaggtgagc ccgccgtcgg tcgtccgggc gcagaacgtc ctgttcgttt
      601 tcgagtcgcc aaccctcgcc gacatcatga cgatgagatc gtgcggcccc agcaccacgt
      661 aatccgtgcg cgcggtgatc tcgccaccgt ggagttcggg gtgccgatgg atgccgccca
      721 gcgtgaacgg accctgccac gtgtcgccgc ggtcccgtga gacaaacacc gctccttccg
      781 gccgtttgcc cccgtggtat ccgacaccgg caaaccggag cgccaagccg ggttcggtga
      841 aatccaacgg cgcttcaagc ggcaccgatg gcgcatccga acccacgaag ttctcgggtt
      901 cgtacgcgcg ccaagtttcg ccctgatcgg tgctctttgc gaggaacgaa tggtacggct
      961 cgttgatatt gtgccccttt ttctcttcgt agccgcccac ggtgaaaccg accagcgctt
     1021 ccttaccttc ccacgaccag agcccgttgt tcgccggcca cgcggcgaat ttctcgactt
     1081 catgatacac aaccacgtgc ttcatttctc gctcctaatc ctccgggtcc cagatggtgc
     1141 cggctatgtg gtgatggaag tgatccttcg tcgcccagta gtacatcgcc atgagcttcc
     1201 cgtcggcacg ctggaacacg cgcggatagc ccatatcggc gtcgttgaac ttgtcgtgtt
     1261 ggaaatcgtc ccgcaacacg gtttcctgcc gccaggtgcg accctcgtcc tcgctgaatt
     1321 tcgccagcat gaccgcctga tcgcggttgc cgaacacgca gcagaggcgc ccatcgcgca
     1381 gcttcgtgag ggcaggcggg ttgccgtttt gcccgccggt gtccccgatc ttgacctgcc
     1441 actgccacgt ggcgccgccg tctgtggagg catacgcgtc aatccagcac gaacgatccg
     1501 cgccgatctc ccgccggcgc accgcggcga ccaaacgacc ggacgcgaag ctgacagtcg
     1561 ccgacccgcg actggcggcg aagctgacag tcgccgaccc gcgactggcg gcgaagctga
     1621 cagtcgcagg catcaccgct cggtacggat cagacggcgg caccatccac gagacgaacc
     1681 ggaacgtgag cccgccgtcg gtcgtccggg cgcagaagac gcggtccacc ataccgccgc
     1741 cctctttccg cgccgacatc aggacgagca tctcccgcgg cccctccacg acgtaatcgg
     1801 tccgggaggt aaagctgagg ccggccagtt cctcatgctg catgaggtcg ccgaatgagt
     1861 acggcccctg ccacgtggcg ccccggtcgt tcgaggcgaa gaacatccct ctcggttcat
     1921 cgctgccgtg gtagccgatc cccacgaccc gcatggcgaa cccgggcgcc gtgaaatcga
     1981 ggggctcagt gagcgcagtc gccgtgatac ccggtctcac gaagttcgcc ggctggaacg
     2041 ccttccatgt atcgccctga ttggtgctgc gcgccagcat cgagcggtag ggctcggtga
     2101 tgttgtgccc gggcttctct tcgaaaccgc ccaccgtgaa gccgacaagc acctctttgc
     2161 catcccacga ccagagtccg ttgttcgccg gccatcccac gaaggcatcc gggtgctcgt
     2221 atacgatgac gtgtttcata ccattttccc ccttgaagcg tttcaggaag ttcatcccag
     2281 cgtcagcctc cagacatcat tcatcaacgg ccacgagttg cccgccacca cccagaggct
     2341 gccgtcgaac acgtacggcg ttgcctcatg gcgcggcgag aaaccgggtt tcgggacgta
     2401 ttcgtgccac gtggtcccgt tttcagtata ccacacgtcg ccaaggttgc agctttcgcg
     2461 gttgctgaat ccgccaatgg tccagagcct gcccgcatac acctcggcaa ccacccactg
     2521 gcgttcgctc cacggcgcgt gtgccgtcac gcattcccag tctgccccgt caaccgagcg
     2581 ccacacatcg ttgtgcgtca cgaaatcggg atagtgcttt tccggcggat cacccttctc
     2641 ctgcgtagcc ccacccatga gccagagcgc gccgtcatgc acggccaccg ccgagccata
     2701 ccgcgggcca aacggcgcat ccacacagac gcactcccat ttctcgccat cccgcgagca
     2761 ccagacatcg ttgccgctgc ccagttgcca catgcagccg ttcagcacga cgacctcgcc
     2821 gtagccccgc actccgaacg gtgtcttgtc cagcacctga ttccacgtca ccccgtcggc
     2881 ggaggtccac acgctaccct tcactgccca gacacggcca tcgtgcacga ccatctcgct
     2941 gtagccgtcg tacggcgtgg catcggatac cattgtccat tcggccccgt cggtggagcg
     3001 ccacaggtcc cgcgagagca cgccgccgta gtagtagccg ttgctgagcc acatggcatc
     3061 gccaaacacg aacggttccg ccgtatcgcg gatgctgaac gccgcctgcg gggttacgag
     3121 gtcccaccgg cggggggatt tctccgatcg tggaggcagg ctcattccag tgtccccgaa
     3181 atatccgacg ggtcgggcac cgttcttccc tcccgtctcg ccgccagtct ggcatctacg
     3241 gaaaaacttg gcgcaccggc gagaagcaca atgcccactg caagccatat tattccaaac
     3301 ggccagagtt cgcggtcaaa gaaagaatac gccaaccaga tgaggagcag aaccgccgca
     3361 gcccatcgcg ttcgatagcc tatcgagaca aacgctccgc agatcgcgat cacgcacgcg
     3421 agcgacgctt ctgcgccctg taattcagaa acgcccataa caccgatcga gaacgcccca
     3481 aaacccacaa ccattcggaa aaccgcaaga aaccaaagcg cgcgctcata agcgtatgtc
     3541 gatagtatcc ggtgtattgg aaatccgaca tccggcgttg cccgatcccg gtcgcggtat
     3601 ttcgagatca gccggcgcaa cggggtattg gtatccacca tgaacctatc ccgcttctgg
     3661 tttgcggtgc agagttaacg cgccatttgc gagttcctct ccaccgtcac gatgtcttcc
     3721 tcgtaaagaa tcgccccctg ctgccgcagt tgcgcctgca acgccgtcac atcaacggcc
     3781 cgcggcgcaa cgccatcccg cagcgaaagc gcgcttgccg tgccggccgc ctctcccatg
     3841 gcgatgcacg gcaccatgac gcggatcgaa cccagcgcga ggtgggtgca gctcacgcac
     3901 cgtccggcag tcagcaggtt ctccgcaccg cgcgggacga ggatgcggta gggaatctgg
     3961 tatttgaagc cacgtgaggg acgccagctt gtggagtcca tgccggggcc atcaatgcag
     4021 tgaatatcca cgaagaacga gccgtaacag attgcgtcgg agaactcgcg ctgacccttc
     4081 acgtcgtcct cggtcagcac gtaatccccc atgatgcggc gcgattcccg aattccgaca
     4141 gtgttcggcg ttgagatcat gtagcagtgt tccataccgg ggacgtactt ccggtagact
     4201 tcgatcgtct cgtacgcctg ttttcgcgct tcaatggtcg ccctggtcag gtcggcggct
     4261 ttcgtcgagt caatgtgcgt cacatgggtg aaattgaccc ccacctgatc gggccgggtc
     4321 ggcgtccacc accaccccat gatggttttc tggaacggcc gcatattgcc ttcccgctgg
     4381 gcgcgctccc acacctcctt caactggtaa tccacgcgga acttctccac ggtcggccaa
     4441 tccacgccac cgatagtgaa cataagggtc accggctggc atttcccgtc tgacggcctg
     4501 cccacttcgt acgggcaccc cgcccggaac gcgacatcgc cgtcgcccgt gcagtccaca
     4561 atccgccgag cgcggataac ctgccgcccg tccttgttct ggataacggc accgatcgca
     4621 gcgccgtcgt ctacgaccgt atcgcagaag aaggtgtggt agagcaggcc gacgcccgtt
     4681 tcctcggcca tggcctccag cagcagcttc atcccctcca cctcgaacat gattccgtgg
     4741 ctgttacgca cgccgaagcc gcccgacacg acacgggagg caatctcgtc ggcaattccc
     4801 ccgacaatcc gcctgccggt gccctcttca tcgtacttcg agatgtgccc gtgcccgccc
     4861 gccgtggcga cgccgccaag gcagttgaac tgctcgacga cgagggtatc ggcccccatc
     4921 cgcgccgcgg ccagccccgc ccccactcct gcgggacctc cccccacgat cagcacgtcc
     4981 acctcgcggg cggtcggcag gtttcggctt tcttccagtg tcatgtgtgt ctcccgtgaa
     5041 gatgcgtccg cccctaccgt actccggcga aaaggcgcgc aataagcgat tctgcggtct
     5101 cccgtggcat ccctgtcata ccggtgatct catccgcgag ggccttctgc aactccgtgt
     5161 ccgccgtaac cccgccatct cgaatccacg tatggagcgg ggcgatgaac gacgcatgat
     5221 gcgcgtgatt ccagtcgaaa tgcggcccgc cctcgcccga aaacacgtac cgatgccgca
     5281 gccaccacgc gatgatgccg gccaccgcgc ctcgcaatcg gacgatagct tccgcgcgtg
     5341 ttcgtggcag cactgcggtg ttgctcagca ccgtgcgcct cgcctcaaca aaatctccga
     5401 gcagaccgag ataggtctcc ggcccgccgg ggatgcacgc ggccaagtcg gatttcagcc
     5461 gcgatccgcg aaccctctcc gacagatcgg cgggcagctc ttccagtcgc gccgtggcgg
     5521 acttccatgc gggcaccagc acggccgcat cgccgccgag aagctgcgca atggcgtgaa
     5581 ggacctcatc gtccgatttc gtcagccatg acgggtcctt cgcgcaccgg gcgaaataga
     5641 tgaggttgtg cagcgcggaa accggctgct gcgcatttcc gaagatgcct ttcgcggcgc
     5701 cggcgtagcg caattcctcg cccacaaccc gccggatgtc atcgaactga atcacgaatg
     5761 cgggcggtgt cggctcgaac tcgatgatct cgtacgtcat cagcgtcgag cgctccacaa
     5821 gccccgcctc ctccaccagt tcgaagttga ttcgcccgtt gccccagcca tcgcggatgc
     5881 tgcgccccgg cagcagctcc cacggttcgg cgggcgggtt ccgcttaagc tcttcaagga
     5941 agggccgcac gagcttacgg agatcgggtt tccacgcgcc ctctcctgcc cagtccgcac
     6001 cccatccgca ccagatccag gggatgagct tcggacgtgt cgccgccaca tcgtcgagga
     6061 tcgcgcgaac agccccgagc atgcggaagt aatcgcgtgg gtgagcgccc gggtagccgc
     6121 cggggtcgcc gtcgatgaac acgtagccgt cagcgttgtt catgcaggca agcaggctgc
     6181 gcatgtgggc gatgtactcg tgaaactggt gctcgtcgtc gaggcggaac gtccgcatga
     6241 acgggtagaa gacgcgatct ttgaccggcg tgccacggat ctcctcccgt gtgctcaggt
     6301 tcgggcagaa ggtgagccag cactccagcc cccgctgtct cgctgtggct atgatgtcac
     6361 gaaagccgcg gaggtgctgt gcgtcgctct cagaaagcgg cggcggggca atttccgaca
     6421 tcggccagat catcacccgg tccatgccga gcccgccgag gagccggaac atggcgtcgt
     6481 aatccgccgg tgcccacgtg cgcaccgcca gcgggtagtt gaacacccag ctcgcgtgga
     6541 ggtaaaaccc cacatggtcg aagcccttca caccctcgtt gcccatgcct ctgtccctct
     6601 cccatccgcg tggcgaacat ttgccgttca tgcgccctgc aggtacctcg cgattgccgc
     6661 gccgacgtcc ggccccaatg accgcgccga ctccgcgttc acctccgcac cggcaacgtt
     6721 tgcgtagggt atctcgatgg cggtggcaag ccgcacgcct ggcagttccg ccgcccagcg
     6781 ggtgaacccc ttcccgccgg tgtagttcgc tgaggaattc cagtcggtcc cgaacggcag
     6841 gaaatcggag gcccggaagg gcagcatccc tttgttctcc gattcgagga tcgcggaaaa
     6901 gcgccgctgt tcggcttcca tcctggggtt ttcggtgccg acaaggtaga tcacctcgtt
     6961 gtgcggaccg gcgatccagg ggcagtgcag gtccagcgca agatggagct tcccgtccgc
     7021 ccaggcggga agttccgccc ggatggcggc ggtttccaca tgcacgctcc ttccgtcgta
     7081 gtcgcggcca tggtcgcggg gaaagcggtt tttgccctga tcgccgtctt ccgagccgtc
     7141 cttgtccacg aacggcacga tgaagaatgc cgcgttcgcg cgcagccatt cagccgtgtc
     7201 ctcgtcgctt tccagcaccc actggatgag ccgctccagt gtgtaattca ccatcatctc
     7261 gcaacagtgg tggcggcagg tgatggcaac gcggtgcgcc ggtttcccgc ccgtcgcgtc
     7321 aaccactgcg tactcggcct cgcgcccttt ccgcgttctg cacagcacgc cggtgctgaa
     7381 cccggggttc cgcgccattc gcgccgcaaa cgccctccag tcagcctcga gatacggcat
     7441 ggcgaagctg aaccgcacct cgtccgcgtc cgccgaaaac gtgtacgaga agcggttgtc
     7501 attcaccggc accgcgccga gccagcgcca gttcctgccc ccgtccggcg acatggccgc
     7561 cccccgcgcg cccagtgccc ggctcctggt gaactggaag gtgagcgtcc gcccggcagc
     7621 gccgcgcacc cggaaatacc agtagaacca gtcggtggtg gtgtcgcgca ggtcctgatg
     7681 cagccgcacg gtatcgccat ccatcgcgtc cacgacgatg ttcccgccgg ggaaatcgca
     7741 atctacagtc acgctcatcg ggtatctccc cccccatacg gcgtccgtgt gaaaatgaac
     7801 cacaaaggcg gagtcggcac aaaggggatg gcgcccggcc gcccgttccc gcagcatcct
     7861 gtattccgta cccagatgag gcaacgcggc gtcttcgtgt caagaggggg aagcagtttt
     7921 ttttgggggg agaggggaat cgcgactgct acgggccgcc atgcgccgct tcttgcgcgg
     7981 gtctccgagt tcggcggaca ggaatgtgcg acccaccgcc attttgccct gcgccccaac
     8041 ggtggggcag gcattcgtgc ctgcccacga tcacgctgtc cctgccaagg ggggcatact
     8101 ggcctggcca cgggcgggcg cgaagcgacg gaccggttgt atcgaggagc cccgcgcatt
     8161 tttgacgggg ttttccatgc cgccaggacg cgacggagcg cgtccctcca tcagactagc
     8221 gtgcaagaat gacggcggtg ttggtgtatt ggtcgtctcg atacagcgaa ccggtcgctg
     8281 agcgtgctgc cccgccgggc agcctgtcga agcgccggtt cgccactcga cgaccgcgcg
     8341 aggccggggc aaggcagtgc gagggcacca ccgcgcctcc ctaccccttc atcccctcgc
     8401 ggacgaacca ctcgccaaag gcatctacgg gaccgtcgaa gaacatgagc gcgccccgga
     8461 tatccgcccg ggcgccaggc gcgaggtcgg gaaagaccgg gtcggcgtgc atgcacgggt
     8521 ggccggggtt accgaccagc gcgttggtgc tctcgaacca cgtcatggcg gcgacgcggc
     8581 cggtttcgcc cgaaacggtc gccatcaccg ggagatcggc agttttcggg ccactgcgcc
     8641 atccgagcga gtagtgaccc gtctcgacgc cggttgcgta cgcctcattg aacggcatcc
     8701 agcctctgcc gggcacatgg acgagcttgt tcgcctgcgt gaactcggcg aactccttga
     8761 tgccgcgcag gtaggcgcat gtctgcagct tgatccgggt cagcggcgcg tccgagccgt
     8821 ttcggatgaa gaggttgagc cgcgcgccga cgccgtccca tcgctccacg gagccaccga
     8881 acgcgatgcc gttggggagg acacgctcga aggaaacccc ggtgtcggtc tgtgtccacg
     8941 gcaccttcgg cgggttcggg aagacgacgg ggaatggcgg gttgatgtgg ctcaggaaca
     9001 ggagcccgga atcggtgaag atcgcctcgg ggaagtccag ccagacgtaa tcctggctcg
     9061 gccacggcgg gcttacccac gcgatttgct cccacgggta gtgtggccgc cacataccgg
     9121 ggaggatacg gatgcccttg gcctgcgttg cgtagtcatc tgccatggaa gaaccctttc
     9181 ccggttaacc gtctggttga ttcccggcct gtgtgaagtc ggccatgcga atgcggtacg
     9241 ccgcctgggc catggggccg tcaaggagcg gatcgccggt gtcgcgcatc cagttgcgca
     9301 ggcgtacgat gagcgcgtcg cgtatcgccc ggtgatcggg gctgtcggcg aggttatact
     9361 gttcatacgg gtccgcttca agatcgaaca actcgtaagg aacatgatac tgcacggaat
     9421 acgggacgtt gagcgccttc gagacctcca cgtagctctt cgcgttgttg aagtaatcgg
     9481 gcgaggtctc ctgccagggc gcgttctcga aattggcgat gagtttccag cggtcggtgc
     9541 gaatgcatct catcggatcg taatacgtgt gatacgtttt ctcggcgaac acggcgtcgt
     9601 tgggcgtgta ctcgacgtct gagaacagcg cggaaaaact cctcccgtgg agattcgtcg
     9661 gaatcggcat gccgagaagg tcgagcagcg tgggcattac gtccacgttg gagatcatct
     9721 cgttgcgcac gatgcccttc gccgcgccgg ggaccgacat gatcagcgcc acctcgatgc
     9781 cggggtcgta gagggtcatc ttcgcgtgga cgaacgggat gccgtggtcg gtggtgaaga
     9841 tgaggatcgt gttgtccgcg gcgggggagg tccggaccgc ctcaacgatc ctcccgaacg
     9901 cccggtccgc cgaggcgatg gaggcttcca tctcggacag gtcctcgcgc acctccggga
     9961 tattcggcat gtaaggcggc acactgacgg aatccggcgg cagggcgtcc acgtcgtcgt
    10021 gcgggaatgg ccggtgcggc tccgcgaagc cgatctgcgc aaaaaacggc ctgccggcgt
    10081 cgtcgcgcga ccgcatgaag gtgatgaaat cgtccgccac cttggcgcag gtgccggtgc
    10141 cgtggatgtg ctcgtgccca caccgctcag gcagccgcgc ctcgtgctgc aggccgaaca
    10201 ggtgggtatc ataccccgcc gcacggagca tctgcgcgag gtgcacttcg tcggggttca
    10261 ggtcgttggc gaacccggcg tgcgtaaggc cgaccacacc gttcgcatgg gggaagcgcc
    10321 ccgtccagag cgcggcgcgg gacgggctgc actgcggcgc ggtgcagaag cttttcgcaa
    10381 accggacacc gaaatctgcg aacgcgtcaa tgtggggcga gcggatgccc ggcacgccgt
    10441 agcagccgag atgctggccg aggtcgtggc agatgacgac gacgaggttg agggggtttt
    10501 gggtcacgga tctgacctca cgagtaggag agcaccacct tcgccgactc acccgacgcg
    10561 aactgtttgt atgctgcggc ggccttcttg aacggaaacc ggtgcgagat gagctttgtc
    10621 acgtcgaggc cgttgcggta gagctcgagc atggcaccga actcggagaa gtgata
//
LOCUS       DAOSOO010000101        10617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6237_length_10617_cov_5.327021, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000101 DAOSOO010000000
VERSION     DAOSOO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_10617_cov_5.327021"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLT86_08365"
     CDS             <1..863
                     /locus_tag="PLT86_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36663.1"
                     /translation="IGVLYGALVLALVAGGCNIFGSSEKEPGSEAARYQQELADFLAL
                     NSTSTISSDVLLALAKESRQAAGIGILQISRELSQSSTSSSTSFPFDSLSAAERAEKL
                     ANLQAMDQALALLLYANMAQAGTGPKVAVDPLSDTYLQQKARNVNLSGTFDVCSVYPD
                     FLVVRILRLMIEFRTKLDAIGTDFRVVGVTGGYKVQYTGSGTVPTAKAIAVNNAIDWL
                     TNPTNGVLSVQINTILDPQSAFGCESPLSDIIDLNKVRSYINTVVVASLKRSKFPVAK
                     PAAEDVVVGE"
     gene            884..1969
                     /locus_tag="PLT86_08370"
     CDS             884..1969
                     /locus_tag="PLT86_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36664.1"
                     /translation="MKRALTFASLIAASVAVVPSVSSASVPHSFRHEVFALGMGGAYT
                     AGAWRGSALFYNPASVAAKRFHMNVPVRFEFGGVGGLTQMKDVVNFFKDNKSDLERID
                     QLPSWRVAELDREAEKLDGKGGTIRAFPAIRLGWRGVGIQSYAVFDGTPQMNSGVFQP
                     RLDLNAYSDIGVIAAYGRPVNLGMLWYAGASVKYFNRWQTIRSFSLEDAVNTPGLGDV
                     VDLKNRQTGIGVDLGALVPVTRKVTLGVVAQDAVTMGDVNPDMSLNLGVHYRVLPRLN
                     LVADYRDLLNASDVSVPMHVHMGGELDLTLLRLRGGFYQGYPTAGVGVNLWLIKLDAV
                     YYGRERGSKLGYEPEDNVAVELQIGLD"
     gene            complement(2038..2868)
                     /gene="rfbD"
                     /locus_tag="PLT86_08375"
     CDS             complement(2038..2868)
                     /gene="rfbD"
                     /locus_tag="PLT86_08375"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT36665.1"
                     /translation="MIVGSGGMLARELARQWSSRHELIALTRQELDVSALENVRGAVR
                     AHAPDVVVNCAAYNRVDDAETHVEDAYRVNALGPRNLAIAAEEQNAALVHFSTDYVFD
                     GKKTTPYLEYDAVNPLGVYARSKVAGETAVRDHCTRFFLVRVAWLVGHGGRNFVEAML
                     HAAKEKGAVSVVTDQVGTPTFCADVARALHTLLETRAFGLYHITGNGSCSWFEFAEEI
                     FRQADLNVSVTPTTMSAYARPAPRPASTILRNLMLELTVGDTMPPWQESLRQYLSTRA
                     "
     gene            complement(2903..3796)
                     /locus_tag="PLT86_08380"
     CDS             complement(2903..3796)
                     /locus_tag="PLT86_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36666.1"
                     /translation="MKFLPAFRRFLVVVLLLSPATGYSWGYKAHQMITAAAIDILPAD
                     MQVVLAPYRAALVAQSHAPDVWRHDPDERPRHYVDLELGDRSGYPFPNFPRDYATALR
                     RLGPDSLKKMGALPWHVDAYFRRTVSSLADPNDSTLVFLSALGHYVADAYMPMHTTVN
                     YDGQLSGNRGVHLRFEWWMIDQNATAIWMKPGLPARITDPLENTWQIVMASYTDVDTI
                     LRADTAVRQSFRPPIPGPRGERSADPPYDQELWNRIGWLAQFRMNLAASAVAGFWYEA
                     WLRAGKPSLQGLSALPPPPMD"
     gene            complement(3793..4266)
                     /locus_tag="PLT86_08385"
     CDS             complement(3793..4266)
                     /locus_tag="PLT86_08385"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOT36667.1"
                     /translation="MTLISNSSNFAARLLDARYVWVSRIVLAVVFLVYGASKIPNLDD
                     FAHSIANYQLLPETLVNALAITLPWIEVIAALALLFRRWAPGAVVAVGGMTLVFTVAI
                     ISALLRGLDINCGCLDTGTTADARNNLLQHLVFDLFLLALAAHVWWWARRNEATR"
     gene            complement(4263..4781)
                     /locus_tag="PLT86_08390"
     CDS             complement(4263..4781)
                     /locus_tag="PLT86_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT36668.1"
                     /translation="MVKKAVIQACLICAGASLIGVGVNLVAPWGIPFVARGRNLPQLP
                     DSALVKGTDPAPSDGHELVSITLQQAKTLFDRDQAVFVDARPEFEYAEGHIPGAINIP
                     FDEVEYYGSAIASLDKHKPLVVYCAGESCDLSIHLGELLAKKGFNTVRVFFGGSVAWQ
                     RAGFPFDRGTGG"
     gene            4946..5641
                     /locus_tag="PLT86_08395"
     CDS             4946..5641
                     /locus_tag="PLT86_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOT36669.1"
                     /translation="MNAQSVLTIILTAGVFLMSGCREMDAELKQHVVAHWSFAVPVTE
                     KLADLAGKHDALVEGVRASEGGGAWYFPGDTAALRVAHTPELDLVNGFTMEATIRLER
                     LGNPRVDIGVQGILEKHIGKGGWLLIVLRDGRVGYWLETEQGRTEGASTIALTPGTWH
                     HVAMTWDKQKIRLYVDGKPAGEQSFAGPLGRCPQDLYLGNDDTKNWSFCGDIADIRIL
                     DIAVEPPAFHRAR"
     gene            5836..7263
                     /locus_tag="PLT86_08400"
     CDS             5836..7263
                     /locus_tag="PLT86_08400"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT36670.1"
                     /translation="MPTEKLDPSLLSDVLRAALRRGADFADIYAEQAAYASVESDDRK
                     INSSFVQDGGVGIRVVKNNRTYYGISASEEPADLMALARQVADAAEAEAARETSVILI
                     EGSTNWPSSFSVPPDMLDVQQKLAIVRSGEETAWSGEKRVRQVSIGYRDSVRRIQLAS
                     SVTGTVLEKELGLTEFSARVFVGDGNQLFVGTAGRSIFGGPEHFQGIDSFESITLRAR
                     RNGITQLEADDAPRGMMPVVFAPGDNGVLFHEACGHGMEADLVERGSAFAGKQGELVA
                     SELVTLYDDGTIPCFPGSFPFDDEGIASQRTTLIERGVLRNYLQSLVTARKSACEPTG
                     NGRRQNYRHPPIPRMRNTYIGAGSMNPEDIIAQTDAGLYAEVHGSGGQVDVITGRFTT
                     SVQVAWLIEKGKRTRPVRGATLSGTGIDVLKNIDMVGNDLVIAQMSGRCGKGQSVPVG
                     VGMPTVRVRSLLVGGKGEVWKGATP"
     gene            7260..8621
                     /locus_tag="PLT86_08405"
     CDS             7260..8621
                     /locus_tag="PLT86_08405"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT36671.1"
                     /translation="MNDMEFARRTLHYAMQRGVQAAEVAVVRTRKTEISVVNMTPETV
                     NAQEEIGFGVRVLKDARMGFAASNRLDSRSVQDLLDSLVSIVGEHTPDEANTLPEWSG
                     GKPWEDRLSTFDEVILRTPLEEKVRRALTLEQCARDFDTRIKGASTVIYGDVAEEEAL
                     VNSRGVEGVSRSTLAYAVIEAIAGDKDSIETGWHVGASSTYDGLDIEATAATAARRAL
                     RMVGSRPAQSAELPLVFPPEVGSDILAYISRMLDADAVQKGKSLFRGREDSQVASSLV
                     SVVDDGRLEGGLATRFADAEGVPTARTELVRDGVLKGFLYDSYTAKKGGTVSTGNAQR
                     GSYAARPHTGTTNFMLLPGSRATDTLLHDVSIGLHVAEMQGLHAAINPVSGDFSIPCK
                     GLMIRGGELAEPVTGVAISGNLFDLLRDVDGVGDDFTWVFSGAIVGTPTFRVRNVKVG
                     SAG"
     gene            8660..8995
                     /locus_tag="PLT86_08410"
     CDS             8660..8995
                     /locus_tag="PLT86_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36672.1"
                     /translation="MTNVLWLVIFWAMQVVAALSFKQGSLPDASRTLWFIIGNVFGAS
                     SIWFMMELYKTMNVNVAMGLAVGGAFLLSQLATALIFESNLSFVQYIGLIAITGGMYA
                     VSAGATTHP"
     gene            9013..10023
                     /locus_tag="PLT86_08415"
     CDS             9013..10023
                     /locus_tag="PLT86_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36673.1"
                     /translation="MTERERFFRELDLDGDGTVNSDVGITYPVWNEYKERLDWMERFS
                     RYVHVGTSRTPSRPAYSKWTDPWGCEWLYPLESLDGLVMRHPVAEWDALTSYKGPNSA
                     EFTDWSEVEKKFKAAKARGEVARGGTDHGFVFLRLTYLRGFNAFMEDVGSRDERLFDL
                     IGVVEDYWFDVAKRYVEAGADLVSFGDDLGLQNRLPISPNAWRTFIKPSYMRIFQHLR
                     SNGVRVHLHTDGYIVDIIPDLIECGVSILNPQELVNGLETLRELAWGRVFLDLDIDRQ
                     SITPFGTPEECERHIGRCVKTLGSPRGGLHLIWGVYPHTPYENIEAVVRAMDSYSEYW
                     KR"
     gene            10043..>10617
                     /locus_tag="PLT86_08420"
     CDS             10043..>10617
                     /locus_tag="PLT86_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT36674.1"
                     /translation="MSAANTVFQLDGRVAIVTGGGRGLGEVFCRTFADAGATVVVADV
                     VLANAERVASDFVSAGHRAVPVHLDVRSTASVNAMVERTIAEFGQVDVLMNNAGIVIN
                     TPAEEMTDAQWEDVLAVDLTGVFKCCRAVAPHMIARKRGSIINIGSMSGVVSNHPQPQ
                     CSYNAAKAGVIMLSKSLAGEWAQYGIRVNTIS"
BASE COUNT         2213 a         2864 c         3366 g         2174 t
ORIGIN      
        1 gaataggagt gttgtatggg gcgctggttc ttgcgctggt ggccggagga tgtaacatct
       61 tcggctcatc cgagaaagag ccgggctccg aagctgcgcg gtatcagcag gaactggcgg
      121 atttcctcgc gttgaacagc accagcacga tatcatccga tgtgctgctt gcgctcgcca
      181 aagaatcgcg ccaggccgcg ggaattggta tcctgcagat ttcgcgcgaa ttgagccaaa
      241 gcagtaccag ttcgagcacc agtttcccgt ttgacagcct ttccgcggcg gaacgcgcgg
      301 aaaagctggc caacctgcag gcaatggacc aggctcttgc gctgctgctg tacgcgaaca
      361 tggcgcaggc agggacgggt ccgaaagttg ccgtagaccc gctttcagat acctatctgc
      421 agcagaaagc gcgaaacgtg aacttgagcg gaacgtttga cgtgtgcagc gtgtatcccg
      481 atttccttgt ggtgcggatc ctgcgtctta tgattgaatt ccggaccaag ctggatgcaa
      541 tcgggacaga cttccgcgtg gtgggtgtta cgggtggcta caaggtgcag tacaccggat
      601 caggtacggt tcccaccgcc aaagcgattg ctgtgaacaa tgcaattgac tggttgacca
      661 acccgacgaa cggcgtgttg tcagtgcaga tcaacacgat acttgatccg caatcagcct
      721 tcgggtgcga gtcgccactg agcgacatca tagacctgaa caaggtccgg tcgtacatca
      781 acacggtggt ggttgcgagc ctgaagcggt cgaaattccc ggtagcgaag cccgccgccg
      841 aagacgtcgt ggttggcgag tagacacgta aggagaacca gcaatgaagc gcgcattgac
      901 tttcgcatct ttgattgcgg cctctgttgc ggtggtgcct tcggtgtcct ctgccagtgt
      961 accgcacagt tttcgtcacg aggtgtttgc gctcgggatg ggtggagcct acacggctgg
     1021 agcgtggcgc gggtcggcgc tgttctataa cccggcgtcc gttgccgcga agcggttcca
     1081 catgaacgtt ccggtgcggt ttgaattcgg aggcgtcggc ggcctgaccc agatgaagga
     1141 cgtcgtgaac tttttcaagg acaacaagtc ggacctcgaa cggattgacc agttgccttc
     1201 ctggcgagtc gccgaacttg accgggaagc ggagaagctg gatggcaagg gcggcacgat
     1261 acgggcattc cccgcgatcc gcctcggctg gagaggtgtg gggattcagt catacgccgt
     1321 ttttgatggc accccgcaga tgaactccgg cgtgttccag ccacggctgg atctgaacgc
     1381 gtacagcgac ataggggtta tcgccgcgta cggtaggccg gtgaaccttg ggatgctctg
     1441 gtacgccggc gccagcgtga agtacttcaa ccggtggcag acgattcgga gcttctcgct
     1501 ggaggacgcg gtcaatacgc cgggtcttgg tgacgtcgtc gacctcaaga accggcagac
     1561 cggtatcggc gtggaccttg gtgcactggt tccggtcacc cgcaaagtga ctctcggagt
     1621 ggttgcgcag gatgcggtca caatgggtga tgtcaaccca gacatgtcac tgaacctggg
     1681 cgttcattac cgggttctgc cacggctgaa cctcgtggct gattaccgtg accttctgaa
     1741 tgcgtcggac gtctcggtcc ccatgcatgt tcacatgggt ggggagcttg atctgaccct
     1801 gttgaggctt cggggtggtt tctatcaggg gtacccgacc gcgggtgtgg gtgtcaacct
     1861 ctggctgatc aagctcgatg ccgtgtacta cggccgcgaa cgagggtcca aacttgggta
     1921 cgaacctgag gacaacgtgg ccgtcgaact tcagataggt cttgattgac cctgatttgt
     1981 cctgaccgaa gatgaagtgt cccccgcggt atatcgcggg ggacactttt tttcgaatca
     2041 ggcgcgcgtt gacaggtatt gcctgaggct ttcctgccag ggaggcatag tgtcgccgac
     2101 cgtcagctcg agcatcaggt tgcgaaggat tgtcgaggca ggccggggcg ccggcctggc
     2161 gtacgcagac atcgttgtcg gggtgacgga aacattcagg tctgcctgcc ggaatatttc
     2221 ctccgcgaat tcgaaccacg agcacgaacc gttgccggta atgtgataga gcccgaatgc
     2281 gcgcgtttcc aggagagtat gaagcgctcg ggcgacgtcc gcacagaacg tgggtgttcc
     2341 gacctggtca gtgacaacag ataccgcccc tttttccttt gccgcgtgga gcatggcttc
     2401 cacgaaattc cttcctccgt ggccaacaag ccacgcgacc cgaacgagaa aaaaacgcgt
     2461 acagtggtct cgtaccgcag tttcacccgc aaccttgctc cttgcgtata ccccaagggg
     2521 gttgaccgcg tcatactcca gatagggtgt tgtcttcttg ccgtcgaaga cgtagtcggt
     2581 ggaaaaatga accagcgcgg cattctgctc ctcagccgcg atggcgagat tccttgggcc
     2641 cagcgcgttc acacggtagg cgtcttccac gtgggtttcg gcatcgtcaa cgcgattgta
     2701 cgccgcgcag ttgactacca cgtcaggagc gtgcgccctc actgcgccgc gcacgttctc
     2761 gagcgcggac acatccagtt cctgcctcgt gagagcgatg agttcatgtc gcgatgacca
     2821 ctgccgggca agctcgcgag cgagcatccc gccggagccg actatcatga ttttcacggc
     2881 actactcccg cgcagcgtac gctcaatcca tgggcggtgg cggcaatgcg gaaagcccct
     2941 gtaacgaagg ctttcctgca cggagccacg cctcatacca gaaacctgcg actgccgagg
     3001 cggcaaggtt cattcggaac tgcgccaacc agccgatgcg gttccacagc tcctgatcat
     3061 acggcggatc tgcggatcgt tccccgcggg gtcccggaat aggcggccga aacgactgcc
     3121 gcaccgcggt gtcggcgcgg aggatggtat caacgtctgt gtaagacgcc atcacgatct
     3181 gccatgtatt ctcgagtgga tcggtgattc gtgcgggcag gcccggtttc atccatatcg
     3241 ccgtggcgtt ctggtcgatc atccaccatt cgaagcgcaa atgcacccca cggtttccgg
     3301 acaactgccc gtcgtagttc accgtcgtgt gcatgggcat gtacgcatcg gcgacataat
     3361 gcccgagggc cgacaggaac acgagcgtgc tgtcgttcgg gtcggcgagg gaactgacgg
     3421 tacggcggaa atatgcgtca acgtgccagg gaagagcgcc cattttcttc agactgtccg
     3481 gtcccagtct ccgtaaagca gtggcatagt cgcgagggaa gttgggaaag gggtatcccg
     3541 aacggtcgcc gagttccaga tccacgtagt gacgtggtcg ctcatccgga tcgtggcgcc
     3601 acacgtccgg tgcatgagat tgtgcgacaa gcgccgcacg atacggtgcc agtacgactt
     3661 gcatatcggc aggcagaatg tctatcgcgg cggcggtgat catctgatgg gctttgtatc
     3721 cccacgaata gccagttgcc ggggacagca agagcacgac gacaagaaat ctgcgaaatg
     3781 ccgggagaaa cttcatcggg ttgcctcgtt tctgcgggcc caccaccaga catgtgcggc
     3841 caatgcgagg aggaaaaggt cgaacacgag gtgttgcaac agattgttgc gggcatccgc
     3901 ggttgtgccg gtgtcaaggc acccacaatt gatatccagg cctcgcagaa gagcgcttat
     3961 gatcgcaacc gtgaaaacca gagtcatgcc tccaactgcc accacagcgc cgggtgccca
     4021 ccggcggaac aaaagcgcaa gcgccgcaat gacctctatc cacggcagag tgatagcgag
     4081 cgcgttgacc agggtttccg gaagcaactg gtagttcgcg atgctgtggg caaaatcgtc
     4141 gaggttcggt atcttgctcg cgccgtacac gagaaagact accgcaagga caattctgga
     4201 cacccaaacg tatctggcgt ccagcaaccg cgccgcaaag ttcgatgagt tggaaatcag
     4261 agtcatcctc cagtcccgcg atcaaatgga aaaccggcgc gctgccacgc gaccgaaccg
     4321 ccgaaaaaga cgcgcaccgt gttgaatccc tttttcgcca gcaattcgcc cagatgaata
     4381 ctcagatcgc acgattcccc cgcgcaatag acaacgagag gtttgtgttt gtcaaggctc
     4441 gcgattgccg agccgtagta ttcgacttcg tcgaagggaa tgttgatagc gccggggatg
     4501 tgtccttctg cgtattcaaa ctcaggcctg gcatcaacga agacggcctg atcacggtcg
     4561 aaaagtgttt ttgcctgctg gagggttatt gaaacgagct cgtgaccatc ggaaggcgcc
     4621 ggatcagtcc ctttgaccag cgccgagtcg ggcagttgag gaaggttcct cccccgcgcc
     4681 acaaacggga ttccccatgg tgcaacgagg ttcactccca ctccgatgag cgacgctccg
     4741 gcgcaaatca ggcatgcctg gatcacagcc ttcttgacca ctctgattct cccgctgcac
     4801 tattgaaatt gtatattcac cgcattatca tggtagccgc tccggtgcgg tgtgtcaatc
     4861 aaccgcacgc agttcgttgc cttggcgccg tcacggtacg gcatgtatca tcaaattccg
     4921 tcaaacgcgt cgggaggaag ggcggatgaa cgcgcaatcg gtactgacaa tcattttgac
     4981 agcaggagtg tttcttatgt ccgggtgcag ggaaatggat gccgaattga aacaacatgt
     5041 agtggcgcac tggtcgttcg cggtgccggt caccgagaaa cttgccgatc tcgcgggaaa
     5101 acacgacgca ctggtggaag gtgttcgtgc gagcgaaggg ggtggtgcct ggtattttcc
     5161 gggggacacg gccgcgctca gggtggcgca cacgccggag ctggaccttg tgaacgggtt
     5221 taccatggaa gccaccatca ggctggagcg cctcggaaat ccccgggtcg acatcggtgt
     5281 tcaagggata cttgagaaac acataggcaa aggcgggtgg ttgcttatcg tactgcggga
     5341 tggccgcgtc ggttactggc tggaaacaga acagggtcgg acagaaggcg ccagtacgat
     5401 agctcttacg ccgggcacct ggcaccacgt tgcgatgaca tgggacaaac agaaaatccg
     5461 gttgtacgtt gatggaaaac ctgccggaga acagtcgttc gcggggccac tcgggcgatg
     5521 tccgcaggat ctctacctcg ggaatgatga cacgaaaaac tggagcttct gtggcgacat
     5581 cgcggatatc cgcatccttg atatcgccgt cgaaccaccg gcgttccaca gagcccgttg
     5641 aatgccgagt tcgaagacgg aacccgtgcc cggatggtgt tctcggtcca gccggggtta
     5701 ccgggtggct gcaccgaacg cgacaacctt cacaatcatc gacaccgggc gcgcctattt
     5761 tcgctacccg tgcccgttcg gaataagaga gcactgacgc gtcccgttcc accagttttc
     5821 gtcaagaacc cgccgatgcc taccgaaaaa cttgacccca gtctcctgag tgacgtgttg
     5881 cgcgcggcgt tacgccgggg tgccgatttc gctgacatat atgcagaaca ggccgcatac
     5941 gcgagtgtcg aaagcgacga caggaagatc aactcctcct ttgtgcagga cggaggtgtg
     6001 ggcattcgcg tggtgaaaaa caatcgcacc tattacggga tctccgcttc tgaagaacct
     6061 gccgacctga tggcactggc gcgccaggtg gcagacgccg cggaggcgga ggcagcgcgc
     6121 gaaacgtccg tcatccttat tgagggctca accaactggc catcgtcctt ctctgttcct
     6181 ccggatatgc tcgatgtgca gcagaaactg gcgattgttc gttcaggcga ggaaacggca
     6241 tggagtggag agaagcgagt caggcaagtc tccatcggct accgcgactc ggtgcgccgc
     6301 attcaactcg cgtcctcggt gacaggcacc gtgctcgaaa aggaactggg acttaccgag
     6361 ttctccgcac gggtcttcgt cggggacgga aaccagcttt tcgtgggaac tgccgggcgg
     6421 tcgattttcg gtggccctga gcatttccag ggtattgaca gtttcgaatc aatcacgttg
     6481 cgggcccgcc ggaacgggat cacccagctc gaagccgacg acgctcccag aggtatgatg
     6541 ccggttgtgt ttgcaccggg ggacaacgga gtgctgtttc acgaggcgtg cggacacggg
     6601 atggaagctg accttgtgga gcgaggatcg gcattcgcag gaaaacaagg tgaactggtc
     6661 gcgagtgagc ttgtcacgct gtacgacgac ggaaccatcc cctgctttcc gggcagcttc
     6721 ccgttcgacg acgagggaat tgcctcgcag agaacaacac tgattgagcg cggcgtgctc
     6781 aggaattacc tccagtcgct tgtcacagcg cgcaaatccg cgtgcgaacc gacgggaaac
     6841 gggagacgcc agaactaccg tcatcctcca attccccgga tgcgaaacac gtatatcggc
     6901 gcaggctcca tgaatccgga ggacataatt gcgcaaacgg acgcggggct ttacgcggag
     6961 gttcatggtt ctggcgggca ggttgacgtg attaccggca ggttcaccac gagcgtgcag
     7021 gtggcgtggc tcatagagaa agggaagcgg acgcggcccg tacgcggagc taccctgagc
     7081 ggtaccggga tcgacgttct gaagaacatc gacatggtgg ggaatgatct tgtcatcgcg
     7141 cagatgtcgg gcaggtgtgg aaaggggcag agcgtgcccg tgggcgtcgg tatgccgacg
     7201 gtgagggtca gatcgctgct cgttggtggc aaaggagagg tctggaaggg agccacgcca
     7261 tgaacgacat ggagtttgcc cgccgaacac tgcactatgc catgcagcga ggggttcaag
     7321 ctgcagaagt ggccgttgtg cgaacccgga aaacggaaat cagtgttgtc aacatgacac
     7381 cggagacagt gaatgcccag gaggagatcg gattcggtgt cagggttctg aaagacgcga
     7441 ggatggggtt cgccgcgagc aatcggctgg attcgcgttc cgtccaggac ctgttggatt
     7501 cgctcgtgtc catcgtgggt gaacatacac ccgacgaagc aaacacgctg ccggaatggt
     7561 ccggcgggaa accctgggaa gacaggcttt caaccttcga cgaggtgatc cttcgtaccc
     7621 cacttgagga aaaggtgcgc agagcgctta ccctcgagca atgcgcgcgc gatttcgaca
     7681 cgcggatcaa aggcgcgagc acggtgattt atggtgacgt ggcggaggag gaagcactcg
     7741 tcaactcacg cggtgtggag ggagtgtccc ggtctaccct cgcgtatgcg gtgatcgagg
     7801 caattgccgg cgacaaggat tccatagaga caggctggca cgtaggggca agctccacgt
     7861 acgacggtct ggacatcgag gcgaccgccg cgacggctgc gcgtcgtgcg ttgcgcatgg
     7921 tggggagcag acccgctcag tccgccgaac tcccgctcgt gtttccgccg gaagtcggca
     7981 gtgatattct cgcgtacata tcgaggatgc tggatgcgga cgcagtgcag aaggggaaat
     8041 cgttgttccg ggggcgggaa gattcacagg tggcctcttc cctcgtttcc gttgtggacg
     8101 acgggcgtct cgagggtggc cttgcgaccc gttttgcgga cgcagagggt gtgccgactg
     8161 cgcggacgga attggtgaga gacggagttt tgaaaggctt tctgtacgat tcttacaccg
     8221 caaaaaaagg cggcacggtt tccacgggaa acgcccagag gggaagctat gccgcgcgtc
     8281 ctcataccgg gacgaccaat ttcatgttgt tgcccgggag tcgcgcgacg gacacattgc
     8341 tgcacgacgt gtcgatcggt ctgcatgtag cggaaatgca ggggctgcat gcggcgatca
     8401 acccggtatc cggcgatttt tccattccct gcaaggggct catgatccgt ggcggagagc
     8461 tcgccgagcc cgtgacgggt gtggcaatca gtggtaatct gttcgatttg ttgcgcgacg
     8521 ttgacggagt tggcgacgac ttcacgtggg ttttctccgg tgcgatcgtc ggaacaccaa
     8581 cgtttcgcgt gcggaacgtg aaagtcggca gtgcaggttg atcggtggcg cggaacgtac
     8641 ctggtggatc gggatgacaa tgacgaacgt actctggttg gtgattttct gggcgatgca
     8701 ggtggttgcg gcgctttctt tcaagcaggg tagcctgccg gatgcgtcgc ggacactctg
     8761 gttcattatc ggcaacgtgt ttggcgcatc aagcatctgg ttcatgatgg agctgtacaa
     8821 gacgatgaac gtgaatgttg cgatggggct ggcagtcggg ggcgcgtttc tgctgtcgca
     8881 actcgctacg gcgctgatct tcgagtctaa cctttctttc gtgcagtaca tcggcttgat
     8941 agctataacc ggtgggatgt acgctgtgtc tgcgggtgca acaacacacc cttgaccggg
     9001 agcggaaaag agatgacgga acgggaaaga ttcttccgcg agctggacct tgacggggat
     9061 gggacagtca actccgacgt tggcatcacc tatccggtgt ggaacgagta caaagagcgc
     9121 ctcgactgga tggaacgctt ctcccgttac gttcacgtag gaacctcgcg cacgccaagc
     9181 aggcccgcgt acagcaaatg gacggatccg tggggttgcg agtggctgta tccgctggag
     9241 tcactggacg ggcttgtgat gcgacaccct gtcgccgaat gggacgcgct gacgtcgtac
     9301 aaggggccaa attccgcgga gttcacggat tggagtgagg ttgaaaaaaa gttcaaggct
     9361 gcgaaggcca ggggtgaggt ggcacgtggc ggcactgacc acggcttcgt gtttctccgc
     9421 ctgacatatc ttcgcggatt caatgcgttc atggaggatg tcggatcgcg ggacgagcgg
     9481 cttttcgacc tcataggcgt cgtcgaggat tactggttcg acgttgcgaa gcggtacgtt
     9541 gaggccggag cggatcttgt ctcgtttggc gatgacctgg ggctccagaa caggcttccg
     9601 atttctccga atgcgtggcg aacattcatc aaaccttcct acatgcggat attccagcat
     9661 ctgcgctcga atggcgtgcg tgttcatctt cacactgatg gatacatcgt ggatatcatc
     9721 cccgatctga tcgagtgcgg cgtttcgatt ctgaaccctc aggagcttgt gaacggcctt
     9781 gagacgctcc gcgagcttgc gtggggcaga gtattcctcg accttgatat cgaccggcag
     9841 agcattacgc cattcggcac gccggaagaa tgcgaacggc acatcggacg gtgtgtcaag
     9901 actcttgggt cgcctcgcgg tggcctgcat ctgatctggg gcgtgtaccc tcacacaccg
     9961 tacgagaata tcgaagctgt tgtacgggcg atggactcgt attcggaata ctggaaacgg
    10021 tgaatcgggg aggaacggga caatgtctgc agcgaacaca gtgtttcagc ttgatggccg
    10081 cgtggcgatt gtcaccggag gcgggcgcgg acttggagaa gtgttctgcc ggacgtttgc
    10141 ggacgcagga gccacagttg ttgttgcgga cgttgtgctg gccaatgcgg aacgagttgc
    10201 gagcgacttt gtgagcgccg ggcatcgagc tgttccggtc catctggacg ttcgttccac
    10261 ggcatctgtg aacgcgatgg tcgaacgtac gattgccgaa ttcgggcagg tggacgttct
    10321 catgaacaac gccggaatcg tcatcaacac gcccgcggag gagatgacag acgcgcaatg
    10381 ggaagacgtg cttgccgtgg atctgacggg tgtgttcaag tgctgccgtg ccgtggcgcc
    10441 gcacatgatt gcgcggaaac ggggttccat tatcaacatc gggtccatgt ccggtgtggt
    10501 gtcgaatcat ccgcagccgc agtgctcgta caacgccgcc aaagccggtg tcatcatgtt
    10561 gtcgaagtca ttggcggggg aatgggcgca gtacggtatt cgtgtcaaca cgatctc
//
LOCUS       DAOSOO010000102        10448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6372_length_10448_cov_5.547484, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000102 DAOSOO010000000
VERSION     DAOSOO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6372_length_10448_cov_5.547484"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLT86_08425"
     CDS             <1..721
                     /locus_tag="PLT86_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT36675.1"
                     /translation="GGGGKAAVVGLRERPEVAKAIDHYNYAYGVSFVRAGFITFCPDA
                     RGFGERQEAAVKGNVLASSCSVLNNMAYPLGQTVTGMWAWDLTRLVDYIETRSDCRRD
                     RIGCAGLSGGGLQTLWATAFDERITCAVISGYLYGYKDSLLDLHQNCSCNYVPHLYEY
                     VDMGDIAATIAPRPILIETGNRDALNGARGVANVTSQVDIIRRSYGVLGVQDRVYHDV
                     FEGEHRWNGVHALPWLEKWLK"
     gene            complement(815..1072)
                     /gene="rpsT"
                     /locus_tag="PLT86_08430"
     CDS             complement(815..1072)
                     /gene="rpsT"
                     /locus_tag="PLT86_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT36676.1"
                     /translation="MANHKSAIKRIRTNREQQARNRQWMSRLRTVVKAVRKASTPEEA
                     KAAFEKAVPIIDSTARKGVIHKNAAARHKARLSNVLKALSA"
     gene            complement(1184..2689)
                     /locus_tag="PLT86_08435"
     CDS             complement(1184..2689)
                     /locus_tag="PLT86_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT36677.1"
                     /translation="MSLLGIDVGTTGTKAVAFNHGGRPIASAYREYPLHSRQKGWMEV
                     DPHEVAAAIKDVISLVAAKTRHDPVSALSVSAMGEAAVPVARDGRFLAPVQVSLDERT
                     ASYADWWRGRISNEDVFRITGHTIYSIFTLPRTQWIKEHWRSVYDQTWKFLCFQEFVF
                     YLLGTEPVTDYSQAARTMGFDVRAKQWSERLFEIAELDPGKFPVAVQSGTLVGKVADT
                     VANELGLPRGVKCVTGGHDQPCNALGAGILTGGSAVYGTGTVDCITPAFDHFVDSTDF
                     IQNNLACYPHVVPDKYCSVAYNSTGGNLLKWYRDTFADTEKRLAAEKGIDPYEVILSD
                     LPDGPSPVMVLPHFTVTGTPWFDTNSRGALLGLKLTTTKGEIVKALIEGTTFEMKLNL
                     EALRRSDVAVERIRSTGGGAKSRLWNQLKADTLGVPVATLQTSEGGSLGTAMLAGVAT
                     GVYGSLAEAASALIHEHEVFEPRPEVSARYSERFAIYRQLYPTLREINHRL"
     gene            complement(2713..5061)
                     /locus_tag="PLT86_08440"
     CDS             complement(2713..5061)
                     /locus_tag="PLT86_08440"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36678.1"
                     /translation="MSTRPTIRVVEPSNHHREKWPVTVSVPFPRSTVTSIGQVHLVDT
                     RDSEIPAQRRIMATWPDGSVKWMLLDFPVELEPRECRDISVEISDYEDGKEPEEGIRI
                     EWAGEELHVDTGVLRCRLAATQGGLLSEVIAHGITFSESPGTVRIAQPGGRMLDLSNG
                     PATLTIEEPGVQRAVIAMRGKHFDLEGNAALDYVLRMVVYANQAIVRWYYTIVNREPA
                     DAVPVSKIQMIQPFSLDTATCWAFVGTDRTRYSMPEGWVSMATDGLETRAHDGNRQSG
                     NLNTLRAEVQMEPFIVLGDATKMITVLPRWCHFLFPKAVHYYGRALRYDIWPDTAGVW
                     NFRRGMAKTHELVLAFSHPAEDYESAMPAVAPVLRPVIATVDPHYIEETNAIPFFFAA
                     RGEAYPQLETHFARGFRSLARAYGMLHYGDANGVGYSAQGRGRHLKGKDAVEEVIWVN
                     NEYDMPYMAMLQFLRTGDRNVWLQTAEPHVWHMMDVDTVHSDPAHPINEGGQSIHLAN
                     HIGPPGYGVDPSHEWVEGLITYHLLTGLEHPREHALALGEHLLRWTEAHRADLNSDWM
                     ASRVTGWALIALTALFEFTRDERYRTCALAHGKGLHERVSGTTGHLTESVSYGFPYRA
                     GFMTNIAVIGLKRLWDISGDDLWKDLALKLIEDQWEHLSLPTGLLVYKELPENRYPSG
                     SATELESMAYAWQWTRDSVYLKRGIQLLRLFGPWRRDSASQGTFFTELPDGGLSQEIR
                     LFPRDSHSFSTFRYELPFLRVLHDTGILKQFEPPEIDLSGVM"
     gene            complement(5088..6785)
                     /gene="nadB"
                     /locus_tag="PLT86_08445"
     CDS             complement(5088..6785)
                     /gene="nadB"
                     /locus_tag="PLT86_08445"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOT36679.1"
                     /translation="MDVQTDFLVIGTGIAGLSFALQAAQLGSVAVVTKRKKDDTNTNR
                     AQGGIAAVLDQVDDFALHVNDTLVAGAGLCHEDVVAHIVREGPRIVRQLVDWGVQFTL
                     TSQGTLALGREGGHSHNRIVHAHDLTGAEIERALLAAIQQCPDVTIFENHVAVDLITE
                     HHLPGRRPARHVTCWGAYVFSAEENRVSRFLARRTVLATGGTGQAWRHTTNPDIATGD
                     GIAMAYRAGARVGNLEFMQFHPTTLYHPHERSFLISEAVRGHGAVLINDRGERFMDSV
                     HPMKSLAPRDIVARAIDREMKTTGKPCVYLDITHVDPDSVKDRFPNIYNKCLSLGIDM
                     TRQPIPVVPAAHYMCGGVVVDITSRTDILNLYACGEVALSGLHGANRLASNSLLEAVV
                     MANGAIEDIKQSGLARAPLPEIPAWDDSDVFDVEEWILLRHDIEEIRDLMWDYVGIVR
                     SDVRLRRAHHRLELIWKEVEEFYRRAKVNAELIELRNMACVAALVVRCAMERKESRGL
                     HFNTDHPMSDERFHHDTLLRARPIRRRKSMPPPHSINGKISEYHEIPGVSTPLRGEF"
     gene            7407..7955
                     /locus_tag="PLT86_08450"
     CDS             7407..7955
                     /locus_tag="PLT86_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36680.1"
                     /translation="MVRRTALLFGVGIMAAGMILAGCSKPPTEEITATQAVIDSVKAL
                     PEVSAYAADQIAAVEATMADVQAKVAEKKYDEARQALQGLADQARAIVATAAANKEAM
                     KGTSEQAIADANSALAAADQQIIKLKRNATPEMKQKVADGKAAAAAAAEAFAAGDYAT
                     AAAKAAEAKAAADAIVNPPAAQ"
     gene            8028..9719
                     /locus_tag="PLT86_08455"
     CDS             8028..9719
                     /locus_tag="PLT86_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT36681.1"
                     /translation="MRQRSSWSPILLVATGAVGFVLFGYVVYPSLAVLWQSLGAADGW
                     TLSHYGRFFSFTSSANLLATWNSVVVSCLSVILAALIGVPLAFLLSTFEFRGRKACSA
                     LVSMPIVLPPLVGVLAFVFLLGESGLVPRGLSNLTGSNSASWALNGIAGVVAVHGYSF
                     SVFFFTFVSSALENRDTSLMEAAASLGAGKWLRLRRVTLPLLMPALVGAAIIVFMTSM
                     ASFSAPLLFAGELRVLSVQLYSTRLSGDLRLAAVQSVVLSGVSLAFLLALRYWNNRRR
                     VTMATKGTRGRRRSLPKGLTRWLIPSAAFAVSVIMVLPHLTLVLLSFVKQGTWTFRSD
                     TWFPTEFTSENYRMALSLARPLANSIQMAFFATLGNLVIGVFIGWLLAAKRIRFGNAV
                     DALVMLPWALPGTVVAVNLLTAFSTGNVFSFGQVLAGSVWLLPLAYFVRNLPVVARSA
                     QSAFAQIDPSLEEAARSLGAGWWLRFRRVTLPLIAPGVLGGAMLAFVTALGEFVASVL
                     LWVPANQPISMAIFGEYREYRLEVAAAYGVLLVAFIGLVFFVSRVAFGSKAASEV"
     gene            9852..>10448
                     /locus_tag="PLT86_08460"
     CDS             9852..>10448
                     /locus_tag="PLT86_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36682.1"
                     /translation="MHARTMVAFLLLHCGVCAASDSFVIARNGRAIAKIVTAPEIDRP
                     FDKGVNNQAVRDLIRVIGVMSGASLPAVQDTLAFNDGPQIHVGQTEFVKRAGLIEPGL
                     PANAYRVVLTYEGEQARLVLAGPTALGTSNAVYDLLTRELGVIWGMENQLFEDIPRRQ
                     TVEVGPVDRTVRPPFPFFFLSGPTAAWQARHRLSSGGLE"
BASE COUNT         1969 a         2993 c         3171 g         2315 t
ORIGIN      
        1 cgggggaggg ggcaaagccg cagtagtggg actgcgcgaa cgcccggagg tcgccaaggc
       61 gatcgaccac tacaactacg cttacggcgt gagctttgtg cgcgccggtt tcatcacgtt
      121 ctgccctgac gcgcgcgggt tcggcgagcg gcaggaggcc gcggtgaaag gaaacgtcct
      181 cgcgtcgtcg tgcagtgtgc tgaacaacat ggcgtacccg ctgggccaga cggtgacggg
      241 gatgtgggca tgggacctca cgcgccttgt ggactacatc gagacgcgtt cggattgcag
      301 gcgagaccgt atcgggtgcg cggggctctc aggtggcgga ttgcagacgc tctgggcgac
      361 ggcctttgac gagcgcatca cctgcgcggt gatcagcggg tacctctacg gatacaagga
      421 ctctctgctg gaccttcacc agaactgctc ctgcaactac gtgccgcacc tgtacgagta
      481 tgtggacatg ggcgacatcg ccgcgacaat tgccccccgg ccgattctca tagaaaccgg
      541 aaacagggac gccctcaacg gagcgcgggg tgttgcgaat gtgacgtcgc aggtggacat
      601 catccggagg tcctacggag tacttggcgt gcaggatcgg gtgtaccacg atgtgtttga
      661 aggtgagcac cggtggaacg gggtgcacgc gttgccgtgg ctcgaaaaat ggctgaaata
      721 gggaggggtt tcgtcgtccg atatgcggcg aaaatgaaag aagccgcgtc gtccgagacg
      781 cggcttcttc gtttccgata tgtggaaaac tcgtttatgc cgaaagtgct ttcaacacgt
      841 tgctgagacg agccttgtgc cgggcggccg cattcttgtg aatgacgccc ttgcgcgcag
      901 tgctgtcaat gattggcact gctttttcaa aggctgcttt cgcctcctcc ggcgtggagg
      961 ctttgcggac agcctttacc acggtgcgaa gcctcgacat ccactgacgg tttcttgcct
     1021 gttgttcgcg attcgtgcgg attcttttga ttgcgctttt gtggttggcc acgccgggct
     1081 ccttatgctt gtctgctcac ctcgtgattc ttcaccaaga atgacgaaca tataaaaata
     1141 gggaacggca tggtcgctgt caatctcgac ctcaacaaac ccgctacaat cggtgattga
     1201 tctctctgag agtcgggtag agctggcggt aaatcgcgaa gcgctcactg taccgcgcgg
     1261 agacttccgg acgaggttcg aatacctcgt gttcatgaat cagcgcggat gctgcttccg
     1321 caagtgatcc gtacacgccc gttgcaacgc cggcaagcat cgccgtgccg agggagcctc
     1381 cttcggacgt ttgcagtgta gctacgggaa cgccgagcgt atctgccttg agctggttcc
     1441 acagacggga ctttgcccct ccgccggtag accgaatcct ttccaccgcg acatctgacc
     1501 ttcgcaaagc ttccagattc agcttcattt cgaacgtcgt gccctcgatg agggctttaa
     1561 ctatctcccc ttttgttgtg gtcagtttga ggccaagcag ggcgccgcgg gaattcgtgt
     1621 cgaaccatgg tgttcctgtt acggtgaaat gaggaagcac cattactggc gaaggcccgt
     1681 ccggaagatc gctgaggatg acctcatagg gatcaattcc tttttccgcg gcaaggcgct
     1741 tctccgtgtc ggcgaatgtg tcccggtacc atttcaacag attgccgccg gtgctgttgt
     1801 aggccacgga gcagtatttg tccgggacga cgtgcgggta acaggcaagg ttgttctgga
     1861 taaaatcggt gctgtccacg aaatgatcga acgccggtgt gatgcaatcc acggtccctg
     1921 ttccgtacac cgcgcttcct ccggtaagaa ttcccgcgcc cagcgcattg cagggttgat
     1981 catggccgcc cgtcacgcat tttactccgc gcggcaaccc cagttcattg gccacggtgt
     2041 ccgcgacctt tcccaccaga gtacccgact gaaccgcgac cggaaactta ccgggatcga
     2101 gttcggcgat ttcgaacaac cgttccgacc attgctttgc gcgaacgtcg aatcccatgg
     2161 tccgagccgc ctgggagtaa tcggtgaccg gttccgtgcc caggagataa aagacgaact
     2221 cctggaagca gaggaatttc caggtctggt cgtagactga ccgccaatgc tctttgatcc
     2281 attgcgtgcg gggcagcgtg aaaatggagt agatagtgtg gccggttatg cggaacacgt
     2341 cttcattgga aatccggccc cgccaccagt cggcgtacga ggccgttcgt tcgtccaggc
     2401 tgacctgaac cggcgcgagg aatcttccat cccgtgcgac aggcacggcg gcctccccca
     2461 tggccgacac ggaaagtgcg ctgacaggat catgccgggt ttttgccgcc acgagggaga
     2521 tcacgtcttt gatagccgcg gcgacctcgt gggggtcgac ttccatccag cctttctgac
     2581 gagagtggag cgggtactcc ctgtatgcgg aggctatcgg tctgccaccg tggttgaatg
     2641 ctactgcttt cgttcctgtg gtgccgacgt cgattcccaa aaggctcatg tccgcctcct
     2701 tgccccgcag tgtcacatta ctccggagag gtctatctcc ggcggttcga actgcttgag
     2761 gattccggtg tcgtggagaa cgcgcaggaa aggaagttca taccggaatg tgctgaacga
     2821 gtggctatcc cgcgggaaca gccgtatctc ctgagacagg ccaccatccg gcaattcggt
     2881 gaagaacgtg ccctgggaag cagaatcgcg cctccacggc ccgaatagcc tcaggagctg
     2941 gataccccgc ttcaaataca ctgagtccct tgtccattgc cacgcgtatg ccatggactc
     3001 aagctccgtt gcgcttccgg aagggtaacg attctccggc agttccttgt acaccaaaag
     3061 ccccgtggga agactcagat gctcccactg atcctcaatg agcttcagtg cgagatcctt
     3121 ccacaggtcg tccccggaga tgtcccagag acgtttcagc ccgatgacgg cgatgttcgt
     3181 catgaaacct gcacggtacg ggaacccgta agagaccgat tccgtcaagt ggcccgtggt
     3241 accgctcacc cgttcgtgga gccctttccc gtgggcgagt gcgcaggtgc ggtagcgttc
     3301 gtcgcgtgtg aattcaaaga gcgccgtcag ggctatcagg gcccagcccg taacccgcga
     3361 ggccatccag tcggaattca aatccgcgcg gtgggcttcc gtccaacgga gaagatgctc
     3421 tcccagagcg agagcgtgtt cccgcgggtg ttcgagcccc gtgaggagat ggtatgtgat
     3481 gagtccttcc acccattcat ggcttggatc aacaccataa ccgggcggcc cgatatggtt
     3541 tgcgagatgg atggattggc cgccttcgtt gatcgggtgc gccggatccg agtggacggt
     3601 atccacgtcc atcatgtgcc atacatgtgg ttccgcggtc tgaagccaca cgtttctgtc
     3661 gccggttctc aggaactgca acattgccat gtacggcatg tcgtactcgt tgttgaccca
     3721 gatcacctct tccacggcat ctttgccttt cagatgcctt cccctgccct gcgcgctgta
     3781 ccccactccg ttcgcatcgc catagtgaag cattccgtag gcgcgcgcca gactgcggaa
     3841 cccgcgggca aaatgcgttt caagctgcgg ataggcttct ccccgcgcgg caaagaagaa
     3901 cggtattgcg ttggtttcct cgatgtagtg cgggtccaca gtagcaatca ccgggcggag
     3961 cacgggtgcg accgcaggca tcgcggactc gtaatcctcc gccgggtggg aaaacgcgag
     4021 gaccaactcg tgtgtcttcg ccattccacg gcggaaattc cacacgcctg cagtatccgg
     4081 ccagatgtca taccgcaacg cgcgaccgta ataatggacg gctttcggga agagaaaatg
     4141 gcaccaccgg ggtagtacgg tgatcatctt cgtcgcgtca ccgaggacga tgaaaggctc
     4201 catctgaacc tcggcccgaa gcgtgttcag gtttccggac tgacgattcc cgtcgtgcgc
     4261 ccgggtctcc agcccgtcgg ttgccatgct gacccatcct tcgggcatgg agtagcgcgt
     4321 gcgatccgtg ccgacgaatg cccagcaggt tgcggtatcc agggaaaaag gctggatcat
     4381 ctggattttg ctcaccggaa cggcatcagc aggttccctg ttcacgatgg tgtaatacca
     4441 tcggacgatg gcctgattgg catacacgac catcctgagg acgtaatcga gcgcggcatt
     4501 cccttccaga tcgaaatgtt tcccgcgcat ggcgatgaca gcgcgttgaa cgccgggctc
     4561 ttcaatcgtg agagttgccg gtccgttcga aaggtccagc atacgcccac cgggctgggc
     4621 aatgcgtacg gttccggggc tttcgctgaa agtgattccg tgagcgatca cctcgctcag
     4681 cagtccgccc tgggtcgccg cgagcctgca acgcagaacg cccgtatcca catgcagttc
     4741 ctcgcccgcc cactcgatgc ggatgccttc ttccggctcc ttcccatcct cgtaatcgct
     4801 gatttccacg gagatgtcgc ggcattcccg cggctcgagt tccaccggga aatccagcaa
     4861 catccacttc accgagccat ccggccatgt tgccatgatc cgacgctgcg ccggtatctc
     4921 cgaatcgcgc gtatccacca gatgaacctg cccgatggag gttaccgtac ttcgcgggaa
     4981 cggcacgctt accgtgaccg gccatttctc ccggtggtgg tttgatggct cgacgacacg
     5041 aattgttggg cgggtcgaca tacgttcagg ctccccaagg ttctggctca gaattccccg
     5101 cgcaaagggg tgctgactcc cgggatctca tggtactcag agattttgcc gttgatgctg
     5161 tgcggagggg gcatggactt ccgcctcctg attggtcggg cgcgcaacag cgtatcatgg
     5221 tggaatcttt cgtccgacat ggggtggtcg gtgttgaagt gcagcccccg actttccttc
     5281 cgttccatgg cgcatcgaac aacaagcgcc gccacacacg ccatgtttcg aagctcgatg
     5341 agctccgcgt ttacctttgc ccgccggtag aactcctcaa cttccttcca gatcagttcc
     5401 agacggtggt gagcgcggcg aagtcggacg tcggaacgca caatgccgac gtaatcccac
     5461 atcaggtctc ggatctcttc gatatcatgc cgaagaagga tccactcctc cacgtcgaac
     5521 acgtcactgt catcccaggc gggaatctcc ggaagaggag cgcgggcaag tccactctgc
     5581 ttgatgtcct caattgcgcc gttggccatg actaccgcct cgaggaggct gttcgaggca
     5641 aggcgatttg cgccgtgcag gcctgaaaga gcgacctccc cgcaggcata gaggttgaga
     5701 atgtccgtcc gcgaggtgat atcgacgaca acaccaccgc acatgtaatg tgcggcaggg
     5761 acaaccggaa tgggctgccg ggtcatgtcg atgcctagtg acaggcattt attataaatg
     5821 tttgggaacc ggtctttgac actgtccggg tctacgtgcg taatgtccag atacacgcat
     5881 ggtttgccgg ttgttttcat ttccctgtcg atcgcgcggg cgacaatatc ccgtggcgcg
     5941 aggcttttca tcgggtggac agagtccatg aaccgctcgc cgcggtcgtt gatgaggact
     6001 gctccgtgtc ctcgaacggc ttcgctgatt agaaaagagc gttcgtgtgg atggtacaga
     6061 gtggttgggt ggaattgcat gaattcgagg tttcccactc gcgctcccgc gcggtaggcc
     6121 attgcgattc cgtcaccggt tgcaatatcg gggtttgtcg tgtgacgcca cgcctgtccc
     6181 gtgcctcccg ttgcgagaac agttctgcga gccagaaagc gagagactcg gttttcttcc
     6241 gcgctgaaca cgtatgcacc ccagcaggtt acgtgacgcg cgggacgccg tcctggcaga
     6301 tggtgttccg tgataaggtc gacggcgacg tggttttcga agatcgtcac gtccgggcat
     6361 tgttggattg ccgcgagcag cgcgcgctcg atctcggcgc cggtaaggtc gtgtgcgtgg
     6421 actatacgat tatgggaatg tcctccttct ctccccagcg cgagcgtgcc ctgtgaagtc
     6481 agggtgaact gaactcccca gtcaacaagc tggcgcacga tgcgcggccc ttcacgaacg
     6541 atgtgcgcaa cgacgtcctc gtggcacaaa cccgcgcccg cgacgagtgt gtcgttgacg
     6601 tgcaacgcga aatcgtctac ttggtccagc accgcggcga taccaccctg tgcgcggttt
     6661 gtgtttgtat cgtctttttt tcgctttgtt acgacggcga cagatccgag ctgcgcagcc
     6721 tgaagcgcga aggaaaggcc tgcgattccg gttccgatga ccagaaaatc ggtctgaaca
     6781 tccatgtggc ttctcgttgg ttcgcgccgc gaggactcgg ggcgcgcgta cgacgtgcgg
     6841 caggagttaa cgttaatgta ccggaggggg caaaacagga acaaggcgaa ggcgggtgac
     6901 cggtgcgaaa ggggaacgga aaagggtatc ctgtgcgggc cgacgaagtt caaactgtgt
     6961 ttgatgaccg accctgcaaa aagttcctgc ccggttctaa gcatggttgg cagaacaaca
     7021 gtctgctaag atattgataa ggataatctt atgggcatgt gtccgtgcaa tagcaatagt
     7081 tgtgccagat gccccaaaca aaaaaatatt ccttgtggac gcctgtgaag tgttcccagg
     7141 acgtgatgga ttttactata ttcattaacg gtaatcccga gggcagtttc ggagccgttc
     7201 gcgcatgagg ggaaacgcgg cgtgaaggcg ctctgcccgc ggaggttcga gctgtagtca
     7261 agtgctggtc aggtggtgag agctataccc gtgaggggtg tcgtgccggc tcctcgcgac
     7321 tagctgtatc ctgtcgctga cgcacaggcc accacagcat tacagcggca caacagtccc
     7381 gatattttcg ggaggaggtt gtatcaatgg ttcgtcgcac tgcattgctc tttggcgtcg
     7441 gcatcatggc cgccggaatg attcttgccg gttgcagcaa gccgcccaca gaggagatca
     7501 ccgctactca ggctgttatc gattcagtga aggcgttgcc ggaagtgagc gcgtacgctg
     7561 cagatcagat cgctgcagtg gaagcgacga tggctgacgt tcaggcgaag gttgccgaga
     7621 agaagtacga tgaagcgcgt caggcgctcc agggcttggc agaccaggct cgcgcgatcg
     7681 tcgcgactgc cgccgcgaac aaggaagcca tgaagggcac gtctgagcag gcaatcgctg
     7741 atgcgaactc ggccctggct gctgcggatc agcagatcat caagctgaag aggaatgcaa
     7801 cccccgagat gaagcagaag gttgccgatg gcaaagccgc tgccgctgcc gctgctgaag
     7861 cgtttgctgc tggtgattac gccaccgccg ccgcgaaggc cgccgaggcg aaggctgctg
     7921 cggacgcaat cgtcaatccg ccggctgctc agtaatccac gttacgaaca gacgttacgc
     7981 gaggtcgcgc atcaatgtgc gcgacctcgt ttcgtgtacg gtgtttcatg cggcaaagat
     8041 cctcgtggtc cccgattctg ctcgtcgcga cgggcgcggt cgggtttgtc ctgttcgggt
     8101 acgttgtgta cccatctctt gcggttctgt ggcaaagcct cggcgcggcg gacgggtgga
     8161 cgctttcgca ttacggcagg ttcttttcgt tcacctcttc cgcgaatctc ctcgcgacat
     8221 ggaacagcgt cgtcgtttcc tgcctttccg tcattctggc tgcactcatt ggcgtgcctt
     8281 tggcctttct cctgagcacg tttgagttcc ggggcaggaa ggcctgcagc gcactcgtct
     8341 caatgccgat tgtcttgcct ccacttgtgg gagtacttgc ctttgtcttt ttgctgggcg
     8401 agagcgggtt ggttccgcgc gggctttcga acctgacggg atccaactcg gcatcgtggg
     8461 cgctgaacgg aatcgccggg gtggttgcag tccacgggta cagcttctca gtcttctttt
     8521 tcacgtttgt aagttccgcg ctggagaacc gtgacaccag tctcatggag gcggcagcca
     8581 gtctcggagc cggaaagtgg ctcaggctcc gtcgcgtgac gttgccactt ctgatgcctg
     8641 cgctggtcgg cgccgcgatc attgtgttca tgacaagcat ggcgtcattc agcgctccgt
     8701 tgttgttcgc cggtgaactt cgtgtgttga gtgtccagct ctactccacc cgcctttcgg
     8761 gtgatctcag gcttgctgcg gtccagtctg tcgtactttc cggggtatcc cttgcgtttc
     8821 tccttgcgct gcggtactgg aacaaccgcc gtcgcgtgac catggcaacc aaaggaacac
     8881 gaggcaggcg gagaagcctg ccaaaaggtt tgaccagatg gttgatcccg agcgctgcgt
     8941 tcgcagtttc ggtgatcatg gtgttgcctc atctcacgtt ggtactgctg tctttcgtca
     9001 aacaaggaac gtggaccttc cggtccgaca cgtggtttcc cacggagttc acatccgaga
     9061 attaccggat ggcgctttct ctggcgcgcc cgcttgcaaa cagcattcaa atggcgttct
     9121 tcgcgacact cggcaatctg gtgatcggtg tgttcatagg gtggttgctg gcagcgaagc
     9181 gtatccggtt cgggaacgcg gtggacgcgc tggtcatgtt gccgtgggcg cttccgggga
     9241 cggtggtagc tgtcaatctt cttaccgcgt tcagcaccgg aaacgtcttc agcttcgggc
     9301 aggttctcgc gggttcggtc tggttactgc ccctcgccta tttcgttcga aaccttcccg
     9361 tggttgcccg ctccgcgcaa agcgcgttcg cccagatcga cccctcactt gaggaagccg
     9421 cgcgaagcct cggcgccgga tggtggttgc gtttccgtcg cgtcacgctt cccctcattg
     9481 ctccgggcgt tctcgggggg gcgatgcttg cgttcgtaac tgccctcggg gaattcgttg
     9541 cgtccgtgtt gctctgggtt ccggcaaacc agccgatatc catggcgatt ttcggggaat
     9601 accgcgagta ccgactggaa gtcgccgcgg cgtacggggt gttgctcgtg gcgttcatcg
     9661 ggctggtgtt tttcgtctcc cgggttgcgt tcggatcgaa ggcagcgtca gaagtgtgag
     9721 caatggaccg gagtcaacta gacataatta gcattatcac gcgtgcttct ctcattgcgg
     9781 ttgtccccgg atgcccctca agtgatcttt gtggagattg ccaaccggct ggtcggaggt
     9841 gaggtgacgt gatgcatgcg cgaaccatgg tggcatttct gctccttcac tgcggggttt
     9901 gcgcagcatc cgacagtttc gtcattgctc ggaacgggcg tgccattgcg aaaatcgtga
     9961 ccgcgccgga aatcgatcgc ccgtttgaca aaggcgtgaa caatcaggcg gtgcgggacc
    10021 tgatacgcgt gatcggagta atgtctggcg cgtcattgcc ggcggttcag gacacgctgg
    10081 ccttcaatga cggtccgcag atacatgtcg ggcagacgga attcgtcaaa cgagcggggc
    10141 tcatcgaacc ggggctgcca gcgaacgcgt atcgcgtggt gctgacgtat gagggagaac
    10201 aggcccggtt ggtgctcgcg ggaccgactg cccttggcac gtcaaacgcc gtgtacgatc
    10261 tactgacccg tgagctgggc gtaatctggg gcatggagaa ccagcttttt gaggacatcc
    10321 cgcggcgcca gacagtcgaa gtgggccctg tagaccgcac cgtccgaccc cctttcccct
    10381 tcttctttct gagcggccct accgcggcgt ggcaggcgcg gcaccggctt tcatccggcg
    10441 gcctggaa
//
LOCUS       DAOSOO010000103        10317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6478_length_10317_cov_5.292536, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000103 DAOSOO010000000
VERSION     DAOSOO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6478_length_10317_cov_5.292536"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1850)
                     /locus_tag="PLT86_08465"
     CDS             complement(141..1850)
                     /locus_tag="PLT86_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT36683.1"
                     /translation="MSAENTPVPQHGTLYYGDNLEVLRQHIKDETVDLVYLDPPFNSN
                     ATYNVLFAEKDGTEAAAQIKAFDDTWRWDTAAAQALDDAMSQGGKLAATMQAFAGMLG
                     QSNMMAYLAMMAPRLAELKRVLKPTGSIYLHCDPTASHYLKLLMDAIFGAQNYRNEIV
                     WKRADAHNDARRQYPKITDRILFYSMTEKCTFGAPFAEFPEKTLRNWYLYLELPDGAV
                     RRMTPEERETQLIPNGARRFNPGDLRSPHPRPNLTYDYKGFKPHANGWAVSREKMEEL
                     ERKGLLVFPKSPDGRIMRKRYLDEQPGVRAGDCWTDISQLRASMAELLGYPTQKPVAL
                     LERIIQASSNEGDVVLDPFCGCGTAVVAAQKLKRRWIGIDITHLAVTLIKTRLRDTFG
                     DAVKFDVVGEPITVYDAEELAKTDPYQFQWWALGLVGARPVEKKKGADQGIDGRLFFR
                     TDTSSKFSQIIFSVKAGHVMAPHVRDLRGVIEKEKAEIGVLISFERPTQPMRAEAASA
                     GFYESPWGKHPRIQLLTVEELLAGKKVDYPQTRFANVTFKTAPAATQADRHEQAGLGL
                     EEE"
     gene            2524..2913
                     /locus_tag="PLT86_08470"
     CDS             2524..2913
                     /locus_tag="PLT86_08470"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT36684.1"
                     /translation="MVSKPEIQALADRIGKAFQPERVILFGSHAYGVPGPHSDVDLLV
                     LLPFEGRNLSKSLEILNAVNPPFSVDLLARRPDDTRRRYEQGDPLIREALDRGEVLYE
                     RSRQGMGGKSRGRFPHRKPRVPRAAKP"
     gene            2825..3175
                     /locus_tag="PLT86_08475"
     CDS             2825..3175
                     /locus_tag="PLT86_08475"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT36685.1"
                     /translation="MNEVVREWVAKAEGDFRTASRECRARQNPNFDAVCFHAQQCVEK
                     LMKAALISAGLVPPKTHDLEQLDRLLKQTHTRWDVDLTDLRYLARASVAFRYPGESAQ
                     TRRKLKKPSPSARA"
     gene            complement(3210..4310)
                     /locus_tag="PLT86_08480"
     CDS             complement(3210..4310)
                     /locus_tag="PLT86_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOT36686.1"
                     /translation="MSDFASFNIRDFGAIGDGRHDDTAAIQKALDEAAATRASVFVPD
                     GVFLSSTIKIPPHVGLIGNPTWSFRHDGGSIIRLGDPSAKCLVDLTGAIGATLNGLCL
                     DGASLAAAGSDGGIHGIFVDKKDSGDQEDTPRIERCRVNHFTGDGVRLTHIWCFSIRS
                     CMFSHNKGDGLSVRGWDGFIMDNWFSGNGGAGYAAREDNASMTMTGNRIEWNAGGGIV
                     IHGGSHYNITGNYIDRSGGAGITLMPRGGRPAHVITITGNVIFRSGAPMWRKLEDLES
                     THLRFEGVRGLVCTGNAMRVWRNDGDSGGEFSPNYGIVLKGLVNSVIKDNTLDYGAMT
                     ELLVDLGGHDENSVIRDNPGCLFVDNHWKDWD"
     gene            4495..6279
                     /gene="dnaG"
                     /locus_tag="PLT86_08485"
     CDS             4495..6279
                     /gene="dnaG"
                     /locus_tag="PLT86_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT36687.1"
                     /translation="MPDDDLKERIRAAVDIVTVIGERIPLKKAGRNFVARCPFHQEKT
                     PSFNVNPERQIYHCFGCGAGGDVFSFLMEHDKVSFPEALRTLAAKAGIALPDRQSARA
                     RTNEQARDIYYRANALAREYFIAALNDPAKGEAARRYLERRGLTKDTIATFGLGFAPD
                     AWDGFMSYARPKGLSYEVLANAGLLVQNEQGRFYDRFRGRVMFPVVNDSGRTVAFGAR
                     TLDPAGEPKYLNSSESPVYQKGRILYGLYHARDAIRQENSVLIVEGYMDVLQLMQAGI
                     RNVVATCGTALTREHGAILRRYAERIILLFDGDEAGIRAASRAGDVLLEAGIEGHVAL
                     LPGGEDPDTLVAAEGPEALRSIAERGMPYLQFRWETLARQNNLATISGRNRAISDMLD
                     VIARVDDEMLRGMMANQIAQWGVTDETLVQRAIARRRRQTVRREQEAAPPQTKRWAPP
                     HREKQLVAWMMDRPGVCQQVAEMGVERFSDPLLQEIAASLIRLKDLGELPSAAALLDE
                     KREDSRWAGAVAELAQMHCDEREIGRAVTQLVASLQLPHLKRQIDTLRARLRVEKGIR
                     ETIEITTEIQRLGREMEILNDLLHSGEN"
     gene            6400..6789
                     /locus_tag="PLT86_08490"
     CDS             6400..6789
                     /locus_tag="PLT86_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT36688.1"
                     /translation="MKKQTQHTVSPVRKGLAKEQVMSAMNQDREGDLLTGEQVFAVLS
                     RHKNALAERYGVNSLGVFGSMARGDSGPSSDVDIVFTTDSPNLFRSVRMKLELERLLR
                     RPVDIVRLRDNMNPRLKARILREARYV"
     gene            6782..6958
                     /locus_tag="PLT86_08495"
     CDS             6782..6958
                     /locus_tag="PLT86_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36689.1"
                     /translation="MSDSDVLKARLEAVLEALQRIPRRFAAIRAPEDFEKTEAGRDNL
                     DSICMVLVASGEAS"
     gene            7159..7392
                     /locus_tag="PLT86_08500"
     CDS             7159..7392
                     /locus_tag="PLT86_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT36690.1"
                     /translation="MRTVQLTLDENLVRDVDRLVKQRNTTRSAFTRDALQEAIKAART
                     REMEEKHRAGYRKLPVRKGEFSVWEGEQEWGDE"
     gene            7389..7700
                     /locus_tag="PLT86_08505"
     CDS             7389..7700
                     /locus_tag="PLT86_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOT36691.1"
                     /translation="MKRGEVRWYKFPQPNKKRPVLILTRNSVIDLLDEVTVAPLTTTI
                     RDIPSEVVLTPVDGVPTECAVNCDHLQTVSKAKIGALITSVSDIKMEEVSEAVAFALA
                     L"
     gene            complement(8212..8904)
                     /locus_tag="PLT86_08510"
     CDS             complement(8212..8904)
                     /locus_tag="PLT86_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36692.1"
                     /translation="MRYALVALCALAASPVFAQTQGDSLVAKFYSSVSVRNPAVTQLI
                     VPRQDGTKDTTGVPFDYEVAAGVSGNYGTIYFVMDYQYERQNAQHFVNQEYSLRQKKT
                     SYEVGMRLRDAEEGNLYILSGYVEGKLFGWVGVGVTRQHLRAWLNDGATLARFSLARN
                     NFHALKMDLVVNAEYETNPTQSRIFFSSAITNFKFGRFAVVPFYRHERVKPPGKPAKS
                     SYNGRVRLTIDI"
     gene            9246..9497
                     /locus_tag="PLT86_08515"
     CDS             9246..9497
                     /locus_tag="PLT86_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT36693.1"
                     /translation="MAGLFSRLSLWFRQWFTTSEKRPEVGRNDLCWCGSGKKYKHCHY
                     EIDLRRGTLSRNTTPGAQREMMERVSKRMEKARARAKKR"
     gene            complement(10077..>10317)
                     /locus_tag="PLT86_08520"
     CDS             complement(10077..>10317)
                     /locus_tag="PLT86_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36694.1"
                     /translation="GGSPLDDRWGSSGWTSADSQNAAWTRQSGSLQTGGCVWGRRAAV
                     SEWWTRSGRCIEEPGAQVARVWIPASASRSTERAE"
BASE COUNT         1910 a         3371 c         3176 g         1860 t
ORIGIN      
        1 cgtcgcttcg cttccgttcc tctgtcggcg cgccactcga cgaccgtaca gggtggcgcg
       61 caacgaccgg tcatcgagtg gcctgcgcgc gcgcgcaggt tatatcgaga tgaccggcaa
      121 gagccgcgcg gggaacagcc ctactcctcc tccagcccca gcccggcctg ctcgtgccgg
      181 tcggcctggg tggcggcggg ggcggttttg aacgtcacat tggcgaaccg cgtctgcggg
      241 taatccacct tcttgcccgc aagcaactcc tccaccgtga gcaactggat acgggggtgc
      301 ttgccccagg gtgactcata gaaccccgcc gaagccgcct ccgcgcgcat cggctgcgtc
      361 gggcgctcga agctgatcag caccccgatc tccgccttct ccttctcgat gactccgcgc
      421 aggtcgcgca cgtgcggcgc cattacatgc cctgccttca cgctgaagat gatctggctg
      481 aatttgctcg acgtgtcagt gcggaagaag agtcgcccgt cgatgccctg atccgcgccc
      541 ttcttcttct ccaccggccg cgcgcccaca agccccagcg cccaccactg gaactggtac
      601 ggatcggttt tcgccagctc ctccgcgtca tacaccgtga tgggctcgcc cacgacgtcg
      661 aacttcaccg cgtccccaaa cgtgtcgcgc aggcgggttt tgatcaacgt gacggcaagg
      721 tgcgtgatgt cgattccgat ccagcgccgt ttcagtttct gcgcggccac cactgccgtg
      781 ccgcacccgc agaacgggtc cagcaccacg tccccttcgt tgctggaggc ctgaatgatc
      841 cgctccagca gggcaaccgg cttctgcgtc gggtaaccga ggagttctgc catggacgct
      901 ctgagttggc tgatgtcggt ccagcaatcc cccgccctga caccaggctg ttcatccaga
      961 taccgtttgc gcatgatccg accgtcaggg gatttcggga aaacgagaag gccttttcgc
     1021 tcaagctctt ccatcttctc gcgcgaaact gcccagccat ttgcgtgcgg cttgaaacct
     1081 ttgtaatcgt aagtcaaatt gggccttgga tgtgggctgc gcaggtcgcc agggttgaac
     1141 ctccttgcgc catttgggat aagctgcgtc tcgcgctcct ctggggtcat tctccgtacg
     1201 gctccatctg ggagctccag gtacaggtac cagttcctta acgtcttctc ggggaactcc
     1261 gcaaaagggg caccgaatgt gcacttttcg gtcatggagt agaaaagaat ccggtcggtg
     1321 attttcgggt attgccttcg cgcgtcgtta tgcgcatcag cgcgtttcca cacgatctca
     1381 ttccggtagt tttgcgcgcc gaaaatggcg tccatgagca gcttgaggta gtggctggcg
     1441 gtggggtcgc agtggaggta gatggagccg gtgggtttga gcacgcgttt gagttcggcg
     1501 aggcgcggcg ccatcatggc gaggtaggcc atcatgttgc tctgccccag cattccggcg
     1561 aaggcctgca tggtggcggc caatttcccg ccctgcgaca tggcgtcgtc cagcgcctgt
     1621 gcggcggcgg tgtcccatcg ccacgtatcg tcgaaggcct tgatctgcgc ggcggcctcg
     1681 gtgccgtcct tttccgcgaa caggacgttg tacgtggcgt tggaattgaa tggcggatcg
     1741 aggtagacga ggtctacggt ttcgtccttg atgtgctggc gcagtacttc gaggttatcg
     1801 ccgtagtaga gggttccgtg ttgcggaacc ggggtgttct ccgcagacat tgcgtgcatc
     1861 cccctgtgcg tgttcccgct gttacgcagc aaacgggcgg tcgttgagcg tgctgccagg
     1921 ccgggcagcc tgtcgaaacg ccgcgccgga cacttcgaca ggctcagtga ccggtgcgtc
     1981 gagcttggag cccacttcat cgcgtccgtt tggatacgca aggaacaggt cacgacgaag
     2041 cgcagcgtcc gacagcggac gatgaccctc cagcccttgc agcgtatgca gattaatata
     2101 attcgggggc gcgacggctc gaaggcgggc gggggcttga ggcgggtcgt ctcgatacaa
     2161 cgcgccggga acgttgcttc gcttccgttc cttcccggcg cgccactcgg cgaccgtctg
     2221 tcggcgccgg attaccgtgt tggcgggcgc attacagcca tctcgcgcgg gtctccgagt
     2281 gacgcctgcg tggtccctga gcctgtcgaa gggcagcagg cgttgtatcg aggagcccgc
     2341 gcggcgggga agaaccccgc gcccccctaa tcccagtcct tccagtggtt atccacgaac
     2401 aggcagccgg ggtgctgtgc gtactgctca tccgtctgcc cagaaggatt gaccacgctc
     2461 gtttcaacgg ttaccatgaa cgtgatgctc tccgttcgag tatgcttggc aagggaacgc
     2521 ggaatggtct cgaaaccgga aatacaggcg ttggcagaca ggattgggaa agcgttccaa
     2581 ccggagcgcg tcatcctgtt cggttcgcac gcgtatggcg tgccgggacc gcattctgac
     2641 gtggatctgc tggttctctt gccatttgag ggccgcaatt tgagcaagtc ccttgaaatt
     2701 ctgaatgccg tgaatccgcc gttcagcgtt gacctgctcg caaggcggcc ggacgacacc
     2761 cggcgccggt acgaacaggg tgacccgctg attcgcgagg cgttggatcg aggcgaggta
     2821 ttgtatgaac gaagtcgtca gggaatgggt ggcaaaagcc gagggcgatt tccgcaccgc
     2881 aagccgcgag tgccgcgcgc ggcaaaaccc taacttcgat gcagtttgtt tccacgcgca
     2941 gcaatgcgtc gagaagttaa tgaaagcggc tctgatttcg gctgggttgg tgccaccaaa
     3001 gacccacgat ctcgaacaac tcgatcgcct tctgaaacag acacacacca gatgggacgt
     3061 cgatctgacc gatctgcgtt atctggcaag ggcttcagtg gcgtttcgtt accccggtga
     3121 atcggcacag acaagaagga agctcaagaa gccctcgcca tctgctcgcg cctgagagac
     3181 cagttgaggc tgcttctggt cccctccccc taatcccaat ccttccagtg gttatccacg
     3241 aacaggcagc cggggttgtc cctgatcaca ctgttttcgt catggccgcc caggtccacg
     3301 agcagctcgg tcatcgcgcc gtagtcgagc gtgttgtcct tgatcacgct gttcacaagc
     3361 cccttgagca cgatgccgta attcggcgaa aactccccgc cggagtcgcc gtcgttgcgc
     3421 cagacccgca tggcgttgcc cgtgcagacg agcccgcgca cgccctcgaa gcgcagatgc
     3481 gtgctctcca gatcctccag cttccgccac atgggcgcgc cgctgcggaa gatcacgttg
     3541 ccggtgatgg tgatgacgtg cgccgggcgg cccccgcgcg gcatcagggt aatgccggcc
     3601 ccgccggacc ggtcaatgta gttccccgtg atgttgtagt ggctgccgcc gtggatcacg
     3661 atcccgccgc ccgcgttcca ctcgatgcgg ttgccggtca tggtcatgga ggcgttgtcc
     3721 tcgcgcgccg cataccccgc cccgccgttg ccggaaaacc agttatccat gatgaagccg
     3781 tcccacccgc gcacggagag cccatccccc ttattgtggc tgaacatgca actgcggatg
     3841 ctgaaacacc agatatgggt gagccgcacg ccgtcgccgg tgaagtggtt cacgcggcag
     3901 cgctcgatgc gcggggtatc ctcctggtcg ccggagtcct tcttgtccac gaagatgccg
     3961 tgaatgccgc cgtcagagcc ggcggcggcg aggctcgcgc cgtcgaggca gagcccgttc
     4021 agcgtggcgc cgattgcgcc ggtgaggtcc accaggcatt tcgccgatgg atcgccgagc
     4081 cggatgatgg agccgccatc gtgccggaag ctccacgtcg ggttgccgat gagccccacg
     4141 tgcggcggta tcttgatggt ggaagagaga aacacgccgt cgggaacgaa cacgctggcg
     4201 cgcgtcgcgg cggcttcgtc cagcgccttc tggatggccg cggtgtcgtc gtgccggccg
     4261 tcgccgatgg cgccaaaatc ccggatgttg aacgatgcga agtcggacat gggagtgctc
     4321 cgttgcgggt ggatgggtca ggtgtgcgcg ttctgaaaag tggacacaaa accgctgtgt
     4381 ggcaacaggt tggccatggt cgtgggcttg cacccggcgc ggtggggagc ctgtccggcg
     4441 gcgttatatt tctgtgtgag gatcttctga tcacccacca acccgcagga cagcatgcct
     4501 gacgacgatc tcaaagagcg gattcgcgcc gccgtggaca tcgtgacggt gatcggcgaa
     4561 cggattcccc tgaaaaaggc gggccgaaat ttcgttgctc gttgcccgtt ccatcaggaa
     4621 aagacgccgt cgttcaacgt gaacccggag cgccagatct accactgctt tggctgcgga
     4681 gccggtgggg acgtgttcag cttcctcatg gagcacgaca aggtcagctt cccggaagcc
     4741 ctgcgcaccc tcgccgcgaa ggccggaatc gcgctccccg acaggcagag cgcccgtgcg
     4801 cgcacgaatg aacaggcgcg ggacatctac taccgggcaa atgccctcgc gcgggagtat
     4861 ttcatcgccg cgctgaacga ccccgccaaa ggcgaagccg cccgccggta tcttgaacgg
     4921 cgtgggctca cgaaggatac catcgccacg ttcggccttg gctttgcccc cgatgcgtgg
     4981 gatggtttta tgtcctacgc ccggccaaag gggctgtcct acgaggttct cgccaatgcc
     5041 gggcttcttg ttcagaacga acagggccgc ttctacgacc ggttccgcgg gcgcgtcatg
     5101 tttcccgtcg tcaacgacag cggccgcacg gtggccttcg gcgcgcgcac gctggacccg
     5161 gccggcgagc ccaaatacct gaactcctcg gaaagccccg tctaccagaa aggccgcatc
     5221 ctctacggcc tgtaccacgc gcgggacgcc atccggcagg agaactccgt cctcatcgtc
     5281 gaagggtata tggacgtcct ccagctcatg caggcgggca tcaggaatgt ggttgccacg
     5341 tgcggaacgg cgctcacgcg ggagcacggc gccatcctgc gccggtacgc ggagcggatc
     5401 attctcctgt tcgacggtga cgaagccggc atccgggccg cctcacgcgc gggtgacgtg
     5461 ctgctggaag ccggcatcga agggcacgtg gcgcttctgc ccggcgggga ggatccggac
     5521 accctcgttg ccgccgaagg gccggaggcg ctgcgctcca tcgccgaacg cggcatgccg
     5581 tacctccagt tccggtggga aaccctcgcc cggcagaaca atctcgccac catctccggg
     5641 cggaaccgcg ccatcagcga catgctggac gtgatcgccc gcgtggacga cgagatgctg
     5701 cgcggcatga tggccaacca gatagctcag tggggcgtaa cggacgaaac actggttcaa
     5761 cgcgccatcg cccggcgccg caggcaaacc gtccgccgcg aacaggaagc ggctcccccg
     5821 cagacgaagc gctgggcgcc accccatcgc gaaaagcagc ttgtcgcgtg gatgatggac
     5881 cggccggggg tctgccagca ggtggcggaa atgggcgtgg agcgcttcag cgacccgctg
     5941 ctgcaggaaa tcgccgcgtc gctcatccgc ctgaaggatc tcggcgaact gccgtcagcc
     6001 gcggcgctcc ttgacgaaaa acgcgaggat tcccggtggg ccggcgcggt tgccgaactt
     6061 gcccagatgc actgcgacga gcgggaaatc ggaagggccg taacccagct tgtcgcgtcg
     6121 ctccagttgc ctcacctgaa acggcagata gacaccctgc gggcacgtct gcgggtcgag
     6181 aagggcatcc gggaaaccat cgaaatcacc acggagattc agcgcctcgg tcgggagatg
     6241 gaaattctca acgacctcct ccacagcggg gagaactgag ccgcaccccg caccgccagc
     6301 gccagagaca ttgacactgc ggcttctgtg cgtacgttta cagctaccgg taccttctca
     6361 ggaaactgtg tcggaacacg caggcgcggg accgagagca tgaagaaaca aacacaacac
     6421 accgtcagcc ctgtccgtaa ggggcttgcg aaagagcagg tgatgagtgc catgaaccag
     6481 gatcgtgaag gcgacctgct caccggcgaa caggtgtttg cggtgctcag ccgccacaag
     6541 aacgcgctgg cagagcgata cggagtcaac tcgctgggtg tgttcggttc gatggcgcgc
     6601 ggagactccg gcccctccag cgatgtagac atcgtgttca cgaccgattc gccgaacctg
     6661 tttcgcagcg tccgcatgaa actggagctg gagcggctgc tccggaggcc ggtggacatc
     6721 gtgcggctac gcgacaacat gaacccccga ctgaaggctc gcatactccg cgaggcccgg
     6781 tatgtctgac tctgacgtgc tgaaagcgcg cctcgaggcc gtcctcgaag cactgcaacg
     6841 gattccccgc cggttcgcgg ccatccgggc accggaggat ttcgaaaaga cggaggcagg
     6901 acgggacaat ctggacagca tttgcatggt cctcgtcgcc tcgggagagg catcctgagc
     6961 gccgtccgcc gttgtggacg gttttcacat ccgacaagcc ggacgcatca aggaaacccc
     7021 cgctgccatc actcccgttg gctacgcccg gccattgcca accggaagca acccgcataa
     7081 gtcccgcaac acatattgac agcctggcta tcatgtgtat gtttatgact ataatcgcca
     7141 acacgcagga gtcaggctat gagaactgtt caactcactc tggatgaaaa cctcgtacgc
     7201 gacgtggatc gacttgtgaa gcagcgcaac accacacgat ccgcgttcac gcgggacgct
     7261 ctgcaggaag caatcaaggc agcacgcacc cgcgagatgg aagagaaaca ccgggccggg
     7321 taccggaaac tgccggtcag gaaaggcgaa ttctcggtct gggaagggga acaggaatgg
     7381 ggggatgaat gaaacgaggt gaggtgcgct ggtacaaatt cccccaaccc aacaaaaagc
     7441 gaccggtact catcctgacg cgcaattcgg taatcgacct tctggatgaa gtgaccgttg
     7501 cacccctcac caccaccatt cgcgacattc caagcgaggt ggttctgacg ccggttgacg
     7561 gcgtccccac ggagtgcgcc gtcaactgcg accatctcca gacggtgtca aaggcgaaaa
     7621 tcggcgcttt gatcacctcc gtgagcgaca tcaaaatgga ggaagtgagc gaggctgtcg
     7681 cgtttgcgct tgcgctctga ccgtccctct ccgcctgcct ctgagtggcg ggacggccag
     7741 aggaacgggg gcggtggtcg cgcggccaac cggcgttgct cagccccgga tcgtcatcct
     7801 gggcgccgtc cgccgttgtg gacggttttc acatccgcca gcgcaaacgc atcaaggata
     7861 cccccactgc caacgctgcc caccgcagtc tatcacagcc gggcgacgcg taccgaggcc
     7921 cctcgtgtcc cccatgccga atcgcccatg ccgccgtcat tcctgctcct cttttgccgt
     7981 cattcccacg gaggcgggaa tccacgccat gccgcttcgg gcgaaaaggg agcctcgtac
     8041 gcacgcctcc tcacggaatc tggaatcgcg gcaccatgta cccgtgtttt ttctggagtt
     8101 ccgcctccgc gggaaggacg ggttactctg cggcaggtaa cgccaaggtg cacggcagag
     8161 cagaatctcc cgcagcgcac gccgcccgac ggactgactc gcgcgcaccg ctcaaatgtc
     8221 gatcgtcagc ctcacacgcc cgttgtagga gctcttcgcc ggcttccccg gcggcttcac
     8281 gcgttcgtgc cggtagaacg gcaccacggc gaaccggccg aatttgaagt tcgtaatggc
     8341 actcgagaag aaaatgcggc tctgcgtggg attcgtctcg tattccgcgt tgaccaccag
     8401 atccatcttc agtgcgtgga agttgttccg cgccagcgag aaccgcgcaa gcgtcgcccc
     8461 gtcgttcaac cacgcccgca gatgctgccg cgtcaccccc acgccgaccc agccgaacag
     8521 cttgccctcc acgtagccgc tgaggatgta gaggttcccc tcctccgcgt cgcgcagacg
     8581 catgcccacc tcgtacgacg tcttcttctg acggaggctg tactcctggt tgacgaaatg
     8641 ctgcgcgttc tgccgctcgt actggtaatc catcacgaag tagatggtgc cgtagttccc
     8701 cgaaacacct gccgccacct cgtaatcaaa cggcacgccg gtggtgtcct tcgtgccgtc
     8761 ctgccgcggc acgatcagtt gcgtgacggc gggattgcgc acggacaccg acgaatagaa
     8821 cttggcgaca aggctatccc cctgcgtctg cgcgaaaacc ggcgacgctg caagcgcaca
     8881 cagggcaacc agggcatatc tcatggcagg ctccagaagg gaatcacgcg gtctggcgcg
     8941 agggtgaaac cggatctacc gccgtaccgc gttgtccgca cccacagggt ccctcgccga
     9001 gtgctccgaa acagccactg acgcaggcgc ggaacgcggt gaagaagtcg aaaatgccgg
     9061 cacggcaagt caaggaaagt gtaggcaatc ggcgcgcccg tgtcaatcgc ggttccgggt
     9121 tgccgcggcc gggttgcccg ccgcgtggag gcaccggggc accgcgggcc tacccgctga
     9181 cggagggcgg tcgtacattt ctttcttgat cccccaccat ctcgaacacg aaagaatgca
     9241 ctgccatggc cggtttgttc tcccgcttgt cgctctggtt ccgccagtgg ttcaccacct
     9301 ccgaaaaacg cccggaagtc ggccgcaacg acctctgctg gtgcggaagc gggaaaaagt
     9361 acaaacactg ccattatgaa atcgacctcc ggcgcggcac gctttcccgc aacaccaccc
     9421 ccggcgccca gcgcgaaatg atggaacgcg tcagcaagcg catggaaaag gcccgcgccc
     9481 gcgccaaaaa acggtaatcc cgaacagcac cctcccgtct ggagggaccc gctccgtcgg
     9541 gtccgcgcag gcacaccacc gcccggtcct gcgaaccggg ctcctcgata cggcgtccgc
     9601 ttcgcgtccg ccactcggag accgccgcgc gcctgcccca cacgcaccct cccgtctgga
     9661 gggacccgct ccgtcgggtc cgcgccgcgt tctaaccgtc ggctgatgtc caaccactcg
     9721 acccccaccg gtcatcgagt ggggaaccgc cggggagggg acgcaacgcg acgttttccg
     9781 ccggttcgtc gtatcgagat gaccgaccat catctcaccg ccccgccctt acgccagccc
     9841 ggtccactct gtccatcccg tccacgaaga cctgaccggc cgctctggag ggacccgctc
     9901 cgtcgggtcc acccctcgtt ctaactgccg ggtccgcgca ggcaccccct tgcccggtcc
     9961 tgcgaaccgg gctccgctat cgcgcgccgt tggcgcttca aggatgccgg ccggcggcac
    10021 gcagccgtct ggcggcaggc accgcaatct gcaggagaac ggaccctcaa aagccgtcat
    10081 tccgcgcgct cggtcgagcg cgaagcggag gcgggaatcc agacccgggc aacctgcgcg
    10141 ccgggctcct cgatacagcg tccgctgcgc gtccaccact cggagaccgc cgcgcgcctg
    10201 ccccacacgc accctcccgt ctggagggac ccgctctgtc gggtccatgc cgcgttttga
    10261 ctgtcggctg atgtccaacc actcgacccc caccggtcat cgagtgggga accgccg
//
LOCUS       DAOSOO010000104        10229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6548_length_10229_cov_5.002359, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000104 DAOSOO010000000
VERSION     DAOSOO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_10229_cov_5.002359"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..766)
                     /locus_tag="PLT86_08525"
     CDS             complement(50..766)
                     /locus_tag="PLT86_08525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT36695.1"
                     /translation="MIPFRDSNPSGTPPVVTVGIILANLAAFAYELSLGPLLEPFIGH
                     YGVIPALVTGAEGYVAGGPTDLVRRFVGAMFLHGGWLHLVSNMWYLWLFGDNIEDRLG
                     HLGFLGFYIGCGVAATSFHVAMDPVSPVPMIGASGAIAGVLGAYLVCFPRARIATFLP
                     VLFMPFVFEFPASLVLGSWFLIQLFSGTAAAAVPAEAGGGVAWWAHVGGFVVGMVSIR
                     LLPCRSCRRQRVYAVRFDRA"
     gene            911..2416
                     /locus_tag="PLT86_08530"
     CDS             911..2416
                     /locus_tag="PLT86_08530"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOT36696.1"
                     /translation="MHDQAPPGTVERGKRPPYLFLGIVPSTSRKGSLVMSDFSREHVV
                     ASVLSTHSEVLQRGKPVVISRAPARLDVMGGIADYSGAMVLEGTLAEATICGVQLRDD
                     QTVRIVSSANDPHGGAERDVTVPLNVLFPARTGSPYARVSSEFPKSSPRHWASYGLGA
                     YTVLMAEHVVGSFPCGATIVVHSNVPLGAGVSSSAALEVAVMKALSHAYGFPMAGIPL
                     ASLCQKVENYVVGAPCGIMDQVTSVLGREGEFLCLKCQPHDVQGFVRLPKGVRVVGMN
                     SDVKHSVGGERYTSARVGAFMGQRIIQEVGLREAGVDPTGGYLANISKADFRKKWARH
                     LPQKMTGVKFLELYQRTNDPVAPVAPQTEYMVRSRAAHPVYENDRVQRFISLMKTATD
                     SGEKAPLIAAGKLMYASHWSYGQRCGLGSRETNTLVSLVRQRGVSEGFFGAKITGGGS
                     GGTVAILCRRDTDGALESIAERYHEISGRSPQLFLRSGPGADQWGVIQVSF"
     gene            2475..2996
                     /gene="rsmD"
                     /locus_tag="PLT86_08535"
     CDS             2475..2996
                     /gene="rsmD"
                     /locus_tag="PLT86_08535"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT36697.1"
                     /translation="MKSPDTRPMTDRVKTSLFDSLNPYLLDALVLDLFAGVGGLGIEA
                     LSRGAAHCTFVERDHLCVEALQTNLRKTRLSDRATVYERDVETALTLMRSEDSPVNIV
                     LFDPPFSLGKPPARTALEALVERLADGFIERGGLLVYHHDNETEGGFKTRRFGVINTR
                     NYGRNRVTILKAL"
     gene            complement(2974..3969)
                     /locus_tag="PLT86_08540"
     CDS             complement(2974..3969)
                     /locus_tag="PLT86_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT36698.1"
                     /translation="MKEAILEKLRCPRCREQISVWNVTEEDGGEIVEGTLACHACGGT
                     FPVHSTIPILLIPGRRAVDEFAATLGREAQKVGFSKAVQSLADGTVEYPATRAFEPAV
                     TAAEVREGRYRASESFWETFSRNRLVQQQQNAIAAHLDAMEEMWLRAEVNFADTVLDV
                     GTGWGGALVPLVERAPQDAMICGFDTAFLNLRVAQGQIERAQYDNVVFVVGDCLNPPF
                     REQLFDAVISWFGIGRIPGFSDYLQGILRLLGPGRAFSCAWTPLVNDLDGLASQADLL
                     GMAERLDIPTRPDETAGFMEAIGFEEVELTTVGPIFILSGRAPVSEGNLRGLSGS"
     gene            complement(4042..4542)
                     /locus_tag="PLT86_08545"
     CDS             complement(4042..4542)
                     /locus_tag="PLT86_08545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT36699.1"
                     /translation="MIKTTLGEIVFRLNDQDAPRHTANFRKLARQGFYNGTTFHRVIP
                     GFMIQGGDPNSKDNDRENDGTGGPGYTIPAEIKALHLRGAVSAARQADQVNPERRSSG
                     SQFFICVAPQTFLDGAYSVFGNVVRGMEVVDKIVSVPRDARDNPLTPVVIRSVTIRTL
                     RAGEQP"
     gene            complement(4976..5569)
                     /locus_tag="PLT86_08550"
     CDS             complement(4976..5569)
                     /locus_tag="PLT86_08550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT36700.1"
                     /translation="MRLRTSFLATLPLVVVSVILTSCGSKSREVAVISTSAGDIVIGF
                     FDKDAPRHVENFKKLAREGFYEGTTFHRVIPGFMIQGGDPNSKDADRANDGMGGPSYE
                     IPAEINMPHKRGTLAAARMGDEVNPERRSSGSQFFICVADAPFLDGQYSAFGEVLSGM
                     DVVDKIVAAPRDDRDNPITPITINKVTLRPAKSGETR"
     gene            complement(5553..5774)
                     /locus_tag="PLT86_08555"
     CDS             complement(5553..5774)
                     /locus_tag="PLT86_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT36701.1"
                     /translation="MPIFEYHCDSCGADFERLIYDRNESVACDCGNKNVKKKLSVFAA
                     QVNAGASSSKAAACDSCCMAGGGSCALKN"
     gene            complement(5830..6162)
                     /locus_tag="PLT86_08560"
     CDS             complement(5830..6162)
                     /locus_tag="PLT86_08560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOT36702.1"
                     /translation="MIPAANFTRRVYDVVRRIPSGMVASYGLVALALDRPRDARQVGR
                     ALFVCREADVPCHRVVNRNGVLTGAWAFETPTEQREKLLSEGVAFDSQGRVDMALHCV
                     SVDELTDP"
     gene            complement(6159..7760)
                     /locus_tag="PLT86_08565"
     CDS             complement(6159..7760)
                     /locus_tag="PLT86_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36703.1"
                     /translation="MAQIGEAENGDLKVKDDCLIGHWKLELDPADASGNNHHGNNTGA
                     RFDGSACWFDGVKGHIEIPSLSSSCVGSGDFTISTWVWTAKGQQDITGDIASSFDPHL
                     RKGFNLSIDDRPGLTSSQSNMRNLFFGIDDGLSEPSWRDCGRPGNAILVFAMCVWQGS
                     LYVGTFEGGANERGHVYRYAGDSAWIDCGSPDGANSVASLAVHQGCLYAGTAHYHASG
                     SSLPDSPNTFPGGKVYRYEGGQRWVDCGRLGDACEAFALSVFQESLYAIPLYSQGVFR
                     YDGESHWEEIGRPGNCRAFSLGVFNGSLFTAGNERAGVWRFDGSQWHDCGLRKDVDQV
                     YSFAVCQGALYAGTWPKAVVYRYEGGKDWADCGRLADELEVMAMAVFNGKLYAGSLPL
                     AQVYRSDGDREWTAVGRLDWTPDVRYRRVWSMAAYEGKLFAGVLPSGHVHSMEAGACV
                     THDSELSAGWNHVAAVKEGNTLSLWVNGVKVSQRHAPRPFDITNDVPLRIGFGQQDVF
                     NGAIRDFRLYGRALNAREIANVAAR"
     gene            complement(7718..8530)
                     /locus_tag="PLT86_08570"
     CDS             complement(7718..8530)
                     /locus_tag="PLT86_08570"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36704.1"
                     /translation="MSSAESLIDIHVHLLPGVDDGPRDIDSSVALIEQGLEDGVGCWV
                     TTPHVLGPFDDRVDRLHQSVFQDLVAEVARRRLPAELHLASEIMFGAAADDIKNHRTG
                     TFGGNGRYFLLEYPMMQFPTAAEEALFAAQLAGLRPIVAHPERNADLARRPDLRARLA
                     HRGILFQINARSLSREARPEDRSAAEHMIRNGLAHFVASDGHDPVQRPAFLRFAYERV
                     AELTSEATARRLFAENPRAAIEGRAIQAIREEDTLPMRWWHRLVRRKTEISR"
     gene            complement(8546..9298)
                     /locus_tag="PLT86_08575"
     CDS             complement(8546..9298)
                     /locus_tag="PLT86_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOT36705.1"
                     /translation="MNAFDRIMATRKTRGAGFWVLIDPDRTAPVEAAARAADAERAGA
                     DVILVGSSLLISDTFGDAVREIRGAVKLPVIIFPGSTAQISADADAILFLSLVSGRNP
                     DLLIGQHVRAAPAIKAVGLEPIPTAYMLVESGTLTTIEYISDTKPIPRQKPDIAMAHA
                     LAAEYMGMRLVYLDGGSGAKEPVPTEMIRACYEYITLPLIVGGGIRSGEAAQAAVQAG
                     ASFVVVGNALEKTSGRSLLTELADAVHSAAPA"
     gene            complement(9441..>10229)
                     /locus_tag="PLT86_08580"
     CDS             complement(9441..>10229)
                     /locus_tag="PLT86_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36706.1"
                     /translation="IRSGSVSGVRVGFDGGAHYILRSEDGSSVVAVDDDISFQGVAGF
                     VEVDANDVSRMTLIGEGVLTCGGRGITRGAGRWRGSIVAVCPAERAVTVQVSESTLSP
                     GKGDYLYVRRRTGERIDSFAYRITKVLEANGKLVRVWLNWSPVIGDGVVKEHTETGFR
                     IQGQMPVSAARAYYRGAHLVNHDRTLCTRLKNIRGGWSTQEPVAHLERVDSSTLRAFP
                     PGSAFTVEEIGVGDVVEVLGWVQCERQPDTTWRVESNGPVVTRF"
BASE COUNT         2151 a         3186 c         2856 g         2036 t
ORIGIN      
        1 gggagatcgt cgattgtgca gggggaatcc gtgagcggaa cacgcggatt caggcgcgat
       61 cgaaccgcac cgcgtagacg cgctggcgtc ggcaggatcg gcacggaaga agtcgaatgg
      121 acaccattcc gaccacgaag ccgcccacgt gcgcccacca ggcaactccg cctccggcct
      181 ctgcaggcac tgcggccgcc gctgttccgc tgaaaagctg gatgagaaac caggagccca
      241 ataccagcga agcgggaaat tcgaacacga acggcatgaa cagtactgga agaaacgttg
      301 caatccgcgc acggggaaag catacgaggt acgccccaag aacaccggca atcgcgccgc
      361 tggcgcctat catcgggacg ggtgaaaccg gatccatggc gacatggaac gaagtagccg
      421 caacgccgca cccgatgtag aaacccagaa acccgagatg tcccagtcga tcctcgatat
      481 tgtccccaaa aagccacagg taccacatgt tgctcaccag atgcaaccag cctccgtgca
      541 gaaacatcgc gcccacaaat cgcctcacga ggtctgtggg gccacccgct acgtatcctt
      601 cagcgccggt cacaagagcc ggtattacgc cgtagtgacc gataaatggc tcaagcagtg
      661 gacccaagct gagttcgtac gcaaaggccg ccagattggc gaggataatg cctaccgtaa
      721 ccacaggggg cgttcccgag gggttgctgt cacggaacgg tatcatgtcg cgctgctcga
      781 ttctgtccgt ctgaagatgg gtggaaccac agggaaagat gttcccgcgt aagaccctgc
      841 agggccccga atgcgtccaa ttcaatatag gcacgcgtca gttcaggcag aacctcaaca
      901 ggtaccgcac gtgcatgatc aggcgccgcc gggaacggtt gagcggggca agcggccccc
      961 gtatcttttt ctcggcattg ttccgtcaac gagcagaaag ggatcgctgg tcatgtcgga
     1021 tttcagcaga gaacacgtcg tcgcatcggt gctttccacg cacagcgagg tgcttcaacg
     1081 gggaaagcct gtcgtgataa gcagggcacc ggcccgcctg gacgtcatgg gcggtatcgc
     1141 ggattattcc ggtgcgatgg tgcttgaagg gacactggca gaagcgacga tttgcggggt
     1201 ccagttgcga gacgatcaga cagttcggat tgtgtcgtcc gcaaacgacc cccacggtgg
     1261 ggcggaacgc gacgtcaccg tgccgctgaa cgttctgttc cccgcgcgca ccggctcccc
     1321 atatgcgcgt gtgagtagcg agttccccaa gtcctccccg cgtcactggg cgtcgtacgg
     1381 actgggcgcg tataccgtgt taatggcaga gcacgttgtg ggttcgttcc cctgtggcgc
     1441 aaccatcgtg gtccactcga acgttcctct gggcgcggga gtgagctcat ccgcggctct
     1501 ggaggtggcg gtaatgaaag cgctgtcgca cgcgtacggc tttccgatgg caggtattcc
     1561 tcttgcgtca ctgtgccaga aagtggagaa ctacgtcgtg ggggcaccgt gcgggatcat
     1621 ggaccaggtc acatctgtcc tcggccgtga gggcgagttc ctttgcctga aatgccagcc
     1681 ccacgacgtg cagggctttg tcagattgcc aaaaggcgtt cgggtcgtcg gaatgaactc
     1741 cgacgtgaag cacagtgttg gaggagaacg ctacacaagt gcgcgggtcg gcgcctttat
     1801 gggccagcga ataattcagg aggtcgggct gcgcgaggcc ggggttgatc ccaccggcgg
     1861 ctacctcgcg aacatcagca aggccgattt ccgcaaaaaa tgggcgcgcc atcttcctca
     1921 aaaaatgacc ggagtcaagt tcctcgagtt gtaccaacgc acgaacgacc cggtggcgcc
     1981 cgtggctccg caaaccgaat acatggtgag gagcagagcg gcacatcctg tgtacgaaaa
     2041 cgatagagtg cagcgtttca tttctttgat gaagactgcg acggattcgg gtgaaaaggc
     2101 tccattgatc gctgcgggaa agcttatgta cgcgtcccat tggagctacg gccagcgttg
     2161 tggattgggg agcagggaaa cgaacacact cgtgagtctc gtgcgccaga ggggcgtttc
     2221 cgagggattt ttcggcgcga agatcaccgg tggggggtct ggcggcacgg tcgcaatcct
     2281 gtgccggcgc gatacagacg gcgctttgga gagtatcgca gaacgatacc acgagatttc
     2341 agggcgttcc cctcagctat tccttcgttc cggccctgga gcagatcaat ggggagtgat
     2401 ccaggtctct ttctgaaaaa ggtgcaatcg tgcgcgtcgt tgcgggaaca gcaaggagca
     2461 tacctctttt ctgggtgaag tcaccagaca cccgacccat gactgatcgg gtcaagactt
     2521 cgttgtttga ttcgctgaac ccgtacctgc tggatgctct ggttcttgac ctttttgccg
     2581 gtgtcggcgg cctcgggatc gaggccctca gccgcggagc agcacactgt acgtttgtcg
     2641 agcgggacca tctctgcgtg gaggcacttc aaaccaatct gcgcaaaaca cggctttctg
     2701 accgcgctac ggtgtacgaa cgggacgtcg aaacagcgct cacgctgatg cgatccgagg
     2761 actctcccgt taacattgtc ctcttcgatc ccccgttttc gttggggaaa cctccggcga
     2821 ggaccgcact ggaagcgctg gtggaaaggt tggcggacgg attcatcgaa cggggaggtc
     2881 ttctggtata ccaccacgac aacgaaaccg agggagggtt caagacccgt cgctttggcg
     2941 tcattaacac ccgaaattac ggtcgaaatc gcgttacgat cctgaaagcc ctctaaggtt
     3001 tccctcggaa acgggggccc tccccgaaag aatgaagatg ggcccgacgg tggtaagctc
     3061 cacctcctcg aaaccaatcg cctccatgaa gccggctgtt tcgtcgggcc gcgtcggaat
     3121 gtccagccgt tccgccatgc cgagaaggtc cgcctgactg gcaagaccat ccagatcgtt
     3181 gacgagtggc gtccaggcgc acgagaaagc ccggcccggc cccagcaacc tcaggattcc
     3241 ttgcagataa tcggaaaatc ccggaatacg gccgattcca aaccacgata tcactgcgtc
     3301 aaacaattgt tcccggaacg gcggattcag acagtcgcca accacgaaca caacattgtc
     3361 gtattgtgct cgctcgatct gtccctgggc aacccggaga ttgaggaatg ccgtatcgaa
     3421 gccgcaaatc atcgcgtcct gcggcgcgcg ttccacgagc ggcacaagtg ccccgcccca
     3481 gccagtgccc acatcgagca ccgtgtctgc gaaattgact tccgcccgca accacatctc
     3541 ttccatcgca tccaaatgcg ctgctatggc attctgttgc tgctggacaa gacgattccg
     3601 ggaaaaggtc tcccagaatg attcgctcgc gcgatagcgc ccctcgcgca cttcggcggc
     3661 cgtcaccgct ggttcgaacg cacgtgtcgc cggatactcg acggtgccat ccgcaagcga
     3721 ctgaactgcc ttcgaaaagc ccaccttctg cgcctctcgc ccaagtgtcg ccgcaaattc
     3781 atccacggcg cgccggcccg gaatgagcag tataggtatg gttgaatgga cgggaaaagt
     3841 tccgccgcag gcgtggcacg cgagcgttcc ttcgacgatt tcgccgccat cctcttccgt
     3901 cacattccat acagatatct gctcgcggca ccgcgggcac cgaagcttct ccagaatcgc
     3961 ttccttcatt tgcactccga acaagacgca aaaggggggc gtgagtgccc cccggaggtt
     4021 ggatgtctag gtttggcgca ctcagggttg ctcgccggct cgtaacgtac ggatggtcac
     4081 tgagcgtatc acgacgggtg tcagcggatt atcacgagca tcgcgcggga cagacactat
     4141 cttgtccacg acctccatcc cgcgtacgac atttccgaac accgagtacg caccgtcaag
     4201 gaacgtttga ggggcaacac agatgaagaa ctgactcccg cttgaccggc gctcgggatt
     4261 cacctgatca gcctgtcgag ccgccgacac cgccccgcgc aggtgaagtg ccttgatctc
     4321 tgcgggaatc gtgtaaccgg gacctcccgt tccgtcgttt tcgcggtcgt tatccttgct
     4381 gttgggatct ccgccctgaa tcatgaatcc agggattacg cgatggaacg tggtgccatt
     4441 gtagaacccc tgtcgagcaa gtttccggaa attcgcagtg tgccggggag cgtcctggtc
     4501 gttgagccgg aacacaattt cacctagggt cgttttgatc accgcgacgt cgtacgtggc
     4561 ttgttcttgt gctgtcgcag tttgagcagc cgcagttgcc gtttcgacca cgtcactcag
     4621 tgcttcctgg tttccagccc gtgccttgac ctggaagaaa taggtcgttc ccggttccag
     4681 cccttcaagc ggagttctgt ggagtgtcgt gattgcaggg acagggatcg tgcgcgaaag
     4741 ggtctcccgg gtggttccca cagcgatctc gccgtctgtg ggaatggacg tggaccagaa
     4801 aacgacgatc ctgtccggtc ctaccgactc tatggtgata ttctcgattc tgggcgctga
     4861 ggcttctttt tcgccggatt ttccacagga catgagactg agaagaggcc acaacaggat
     4921 cagcaggctg attctcgagc gcatggcaga acgtactccc cgcttgagtg ttttctcaac
     4981 gcgtttcccc ggattttgcc ggacgcaatg ttactttgtt gattgtaatc ggtgtgatgg
     5041 ggttgtctcg gtcatctcgt ggcgcagcga cgatcttgtc tacgacatcc ataccgctca
     5101 gaacctctcc gaaagccgag tactgcccgt ccaggaatgg tgcatccgcc acgcagatga
     5161 agaactgact cccgcttgat ctccgttcag ggttgacttc atcgcccatt ctcgctgcgg
     5221 caagagttcc ccgtttgtgc ggcatattta tctccgccgg aatctcatac gaaggcccac
     5281 ccatgccgtc attcgccctg tcggcatctt tgctgttcgg gtccccgccc tgaatcatga
     5341 aaccggggat cacgcgatga aacgtagtcc cttcatagaa gccttctcgc gccaacttct
     5401 tgaagttctc cacgtgtctg ggggcatcct tgtcgaaaaa cccaatcact atgtcgccgg
     5461 cagaagttga aatcaccgcc acttcccgcg atttcgaccc gcaggaggtg agaatgacag
     5521 acacgacgac aagaggaaga gtggcaagga agctagttct taagcgcaca cgaacctcct
     5581 cccgccatac agcatgaatc gcacgccgcc gccttggaac tggaagcacc cgcgttgact
     5641 tgagcggcaa aaacgctcag cttcttcttc acgttcttgt tcccgcaatc acacgcaacc
     5701 gactcattcc ggtcatagat caaacgctca aaatctgctc cacacgaatc gcaatggtat
     5761 tcaaagatcg gcatgtttct gcctcctgtt attgaaaaac ccctacattc ctataccaag
     5821 ataacaccgt cacgggtcgg tcagttcatc tacgctgacg caatggagcg ccatatcgac
     5881 acgtccctgg gaatcaaagg caacaccctc tgaaagcagc ttctcccttt gttccgtggg
     5941 cgtttcgaag gcccacgctc cggtgagaac cccgttccga ttcaccaccc tgtggcacgg
     6001 aacatccgcc tccctgcaca cgaacagggc gcgaccaacc tgccgcgcgt cgcgcgggcg
     6061 gtccagcgcc agggcaacca gtccgtacga tgccaccatg ccgctcggaa tcctacgcac
     6121 gacgtcgtac acgcgccggg tgaagtttgc ggcgggaatc atcgggctgc aacattcgct
     6181 atttcgcgag cgtttagtgc acgcccgtag agcctgaaat cccgtatggc gccgttgaac
     6241 acgtcctgct ggccaaatcc gatgcgcaag ggaacatcat tcgtgatgtc aaatggtctt
     6301 ggagcgtggc gctgggacac cttcaccccg ttcacccaga ggctcagtgt gtttccctct
     6361 ttcaccgcag cgacgtggtt ccaaccagca ctcagttccg aatcgtgggt aacgcatgca
     6421 cccgcttcca tcgaatgcac atggccactc ggaagcactc ccgcgaagag cttcccctcg
     6481 tatgcggcca tcgaccacac ccgcctgtac cgaacgtcgg gagtccaatc gagccttccg
     6541 acggctgtcc attctcggtc gccgtcagat cggtacacct gtgccagcgg caggcttccc
     6601 gcgtacagct tgccgttgaa cacagccatt gccatcactt ccagttcgtc cgcaagcctt
     6661 ccgcagtccg cccagtcctt gccgccttcg tacctgtaca caacggcttt gggccatgtc
     6721 cccgcataaa gggccccttg gcaaacggca aaagaataca cctggtctac gtctttccgg
     6781 aggccgcaat cgtgccattg cgatccatca aaccgccaga cgcccgctcg ttcgttgccg
     6841 gccgtaaaga gggaaccatt gaaaactccc agggaaaatg cacggcaatt ccccgggcgc
     6901 ccgatctcct cccagtgcga ttcaccgtcg taccggaaga cgccctgcga atagagcgga
     6961 atcgcgtaca ggctttcctg aaacacactc aacgcaaatg cctcgcaggc gtcgccaagg
     7021 cgaccacaat ccacccagcg ttgcccgccc tcgtagcggt acacctttcc cccggggaag
     7081 gtgttcggcg aatctggtag cgacgatccg gatgcgtgat aatgcgctgt tcccgcgtaa
     7141 aggcacccct gatgaacggc cagtgaggcg acgctgttcg cgccgtccgg gctcccgcaa
     7201 tcaatccagg cagaatcgcc ggcgtaacgg tacacgtggc cccgctcgtt agcccctcct
     7261 tcgaatgtcc ccacatacag gcttccctgc cagacgcaca tcgcgaacac aaggatggcg
     7321 ttccccggtc gtccgcaatc gcgccacgaa ggctcggata gtccgtcatc gattccgaag
     7381 aagagattcc gcatgttgga ttgggaagac gtgaggccgg gtcggtcatc gatactgagg
     7441 ttgaatcctt tcctgagatg gggatcaaac gaggaggcga tatcgccggt aatatcctgc
     7501 tgtcctttcg ccgtccacac ccaggtcgaa atcgtgaagt ccccgcttcc aacacaggag
     7561 gacgacaacg acgggatctc aatgtgaccc ttcactccgt caaaccagca ggctgacccg
     7621 tcgaagcgag cgccggtgtt gttcccgtgg tggttgttac cactcgcatc ggccgggtcc
     7681 agttccagtt tccagtgccc gatcaggcag tcatccttca ccttgagatc tccgttttcc
     7741 gcctcaccaa tctgtgccac cagcgcatcg ggagagtgtc ctcctcccgg attgcctgaa
     7801 tggctctacc ctctatcgct gcccgcggat tctctgcgaa aagcctccgg gcggtagctt
     7861 cgctcgtaag ttccgcgaca cgctcgtacg caaagcgcag aaacgcgggc cgctgcaccg
     7921 gatcgtgccc atccgacgcg acaaaatgcg ccagaccatt acgaatcatg tgctccgcgg
     7981 cgcttcgatc ctctggccgg gcttctcgtg aaaggcttct tgcattgatc tggaacagaa
     8041 tcccgcggtg agcgagccgc gcccgaagat caggccgccg ggcaagatcg gcgttgcgct
     8101 caggatgcgc gacaatcggg cgcaagccgg caagctgcgc ggcgaaaagc gcttcttcag
     8161 cagcggtggg gaactgcatc atcggatatt ccagcaggaa ataccgcccg tttccaccga
     8221 aggtgccagt cctgtggttc ttgatgtcgt cggccgcggc gccaaacatg atctcggacg
     8281 ccagatgaag ctcggcaggc aatcttcggc gggcaacttc ggcgacaaga tcctggaaaa
     8341 ccgattgatg cagcctgtca acgcggtcgt caaaggggcc cagaacgtgc ggagttgtga
     8401 cccagcatcc gaccccgtct tccagaccct gttcaatcag agccaccgag gagtcaatgt
     8461 ctcgcggccc gtcgtctaca cccgggagga ggtggacatg gatatctatc agactttcag
     8521 cggaggacat cagaagtgcc gattttcacg cgggagcggc cgaatggaca gcgtcagcga
     8581 gttccgtcag gagggaccgg ccacttgttt tttcgagagc gttgcccaca accacgaagg
     8641 atgcgcccgc ttgtacggct gcctgcgcgg cttcgccgga acgaattcca cctcccacga
     8701 tcaacggcaa cgtgatgtat tcgtaacacg ccctgatcat ctccgtcggg accggctcct
     8761 tcgctccgct gcccccgtcc agatacacca gtctcatccc catgtattcc gccgcaagag
     8821 catgtgccat tgcgatgtcc ggtttctgcc gcggaatcgg cttcgtgtca cttatgtatt
     8881 cgatcgtggt caacgtgccc gattcgacca gcatgtacgc cgtgggtatg ggctcaagac
     8941 caaccgcctt gatcgcgggg gctgcccgca cgtgttgacc tatcagaagg tcaggattgc
     9001 gcccgctgac gagcgaaagg aacaatattg cgtccgcgtc cgctgaaatc tgggccgtac
     9061 tcccgggaaa aatgatcacc ggcaacttga ccgcgccgcg gatctctcgc accgcgtcac
     9121 cgaatgtgtc cgaaatcagc aggctgctcc ccacgagtat gacatccgca cccgcccgtt
     9181 ccgcgtcggc tgcgcgcgca gccgcttcga caggtgcggt gcggtccggg tcaataagaa
     9241 cccagaaacc ggcaccgcgg gtcttccgcg tcgccattat ccgatcaaaa gcattcacca
     9301 gcatgttccc aggaagaatc agcgtacacg tgcaaacgcc cctcaggagt cgttatcctt
     9361 ccgcgtaagt cttcgtcaaa tatacaaaag ctccggttgt ctggtaacgt ggtcgaggaa
     9421 acctttccga agaacggcca ctagaatcgt gtgaccaccg gtccattgct ttccacgcgc
     9481 cacgtggtat cgggttggcg ctcacattgc acccaaccca gcacttccac cacgtctccc
     9541 accccgatct cttccacggt aaaggccgat ccaggcggaa atgcacgtag tgtggacgaa
     9601 tcaactcttt ccagatgcgc gaccggttcc tgagtcgacc acccaccgcg gatgttcttc
     9661 aaccgcgtac acagggtgcg atcgtggttg acaagatggg ccccccggta atacgcccgc
     9721 gccgcggaaa caggcatctg cccctgaata cgaaagcccg tttccgtgtg ctccttcacg
     9781 actccgtccc cgatgacggg cgaccagttc agccacactc tgacaagctt gccgttcgct
     9841 tcgagcactt ttgttattcg gtatgcgaat gagtcgattc tttcgcccgt tcgccttcgc
     9901 acgtagagat agtccccctt gccgggggac aacgtgcttt cggacacctg gaccgttacc
     9961 gcgcgctcgg cggggcagac agcaacgatc gatccgcgcc accggcctgc ccccctggtg
    10021 atgccgcggc ccccgcaggt caaaaccccc tcgccgatca acgtcattcg ggacacgtcg
    10081 ttggcgtcca cctcgacgaa tcccgcaacg ccctgaaaag agatatcgtc gtccaccgcg
    10141 accacagagg aaccgtcttc cgaacgaagt atgtagtgcg cgccaccgtc aaacccgacc
    10201 cgtacgccgc tcacggaccc cgaacggat
//
LOCUS       DAOSOO010000105        10199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6575_length_10199_cov_5.207906, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000105 DAOSOO010000000
VERSION     DAOSOO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_10199_cov_5.207906"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..443)
                     /locus_tag="PLT86_08585"
     CDS             complement(30..443)
                     /locus_tag="PLT86_08585"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT36707.1"
                     /translation="MRITGYYSFESAHRLHDAGCSNEENAALYGPCQTIHGHTYRLEV
                     TLEGSKLEHGMFLNFERVDEIVRDHVIKRLDHHMIDDEPYFRSHPSTAEELARWIWGQ
                     IEPHFARLAGCRLAEVKVFEGEHFSACVDRSDFEH"
     gene            complement(509..1489)
                     /locus_tag="PLT86_08590"
     CDS             complement(509..1489)
                     /locus_tag="PLT86_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36708.1"
                     /translation="MDLTSRERFVATFAGTSRCSPPLWGDGIRDDVVEDWASQGVCPD
                     ADWERTLGIERFEVVQPDLRHVDFSFTPVTNPEPVQTCPADDIRRYPRDWDQQVVSFS
                     TRDFAVGLRVSRGLFLTFGVADWNSLAPLLIALHDEPETMSHRLREAAEFAMQVLQRA
                     YDSVKFDFVVFSEPIASNCAPVISPEMLRGVCGAAYKVLLDQARKAGVSHVVFQTYGN
                     TASLIPAFRDLGASIFWSGESGVAGVSYPRIRNRYGGALGLIGGIDSAILSLNENEME
                     RRLELDVVPLLEQGRYLPLLDGRVRRGVSYQSYAKYRHLLKDVVARACGP"
     gene            complement(1480..2076)
                     /locus_tag="PLT86_08595"
     CDS             complement(1480..2076)
                     /locus_tag="PLT86_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36709.1"
                     /translation="MVDETKASAGGAVVKTLDDLPGVGPTKAKWLKAAGIKDLQAARD
                     VPLEKLMHVRGIGRLLAVRIKEVLTGQEIGSDATASTDAQASDLTEGQMQWQETVTEH
                     QGSIMVLVSDLLAQPQKYGLKPKFMKDLEKLGAVVDALPVSTPPIDGTRRGKIVKHLK
                     AIQVLLESAVKMDEDSDKHRKVVKEKLRDRRKKLERWT"
     gene            complement(2099..2824)
                     /locus_tag="PLT86_08600"
     CDS             complement(2099..2824)
                     /locus_tag="PLT86_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOT36710.1"
                     /translation="MAKRRFMGTGLPYVDLSEVNGHLLVVEGSDGVGRTTQLSLLRTW
                     LEVQGYGVIETGWTRSALVRESIDLAKAGNALNLLTFNLLYATDFADRLEHEIIPALR
                     SGFVVLADRYVYTAFARALARGTDPEWIRRLYGIAIEPDLVVYLRVDVNTLIHRVLLA
                     DSLDHWEAGLDQYPQLDPYESFIKYQSDVIREFDKMSDEYGFTVIDARKKVDEIQREL
                     RAAISATLSQSAGADVVAPTVDE"
     gene            complement(2840..3595)
                     /gene="tmk"
                     /locus_tag="PLT86_08605"
     CDS             complement(2840..3595)
                     /gene="tmk"
                     /locus_tag="PLT86_08605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT36711.1"
                     /translation="MARLHEKHTFPGRLIIVEGIDGSGKTTQASLLRQWLVSLGVPVF
                     WTEWNSSALVKSSTKKAKKDNTLTPTTFSLLHVTDFADRLSYKIIPPLQAGMVVIADR
                     YAFTAFARDTARGVHPEWVRSLYDFAIKPDLAVYFKVPIRVSLRRLLSARMKLKFYEA
                     GMDTGLSKDPVECFELFQSRVLDEYDRMSEEFGFHVIDAVQPIEAQQKQFRALVSREL
                     QSYLAPYLAAKEQKTGNESKDSTVRRAGTQNDG"
     gene            complement(3579..4241)
                     /locus_tag="PLT86_08610"
     CDS             complement(3579..4241)
                     /locus_tag="PLT86_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT36712.1"
                     /translation="MGEKGLESASIPRSARKAAVIELARSWDFEEAHAVQVTKLALEL
                     YGGLPPPKKNREWEREWLEYASLLHDIGYINGVRRHHKVSEAMICRADLIGFRRSEKQ
                     IIAAVARAHRKRFPDADDPVFDGLGDDDRESVRRLAGVLRIADGFDRTHRGLITSAQM
                     RVSRNDCTISCKAVGGVDEELRAAGRKSDVLQELLGVPVKFIVVSSGGFPEREGENGT
                     TP"
     gene            complement(4234..5739)
                     /locus_tag="PLT86_08615"
     CDS             complement(4234..5739)
                     /locus_tag="PLT86_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOT36713.1"
                     /translation="MHRASLIALRARHHDDTSHSDHVARLATTIFLGTLPAHKVSPLY
                     LPALQAGALLHNVGFFEDPENHHLRGRDIVANEGLRGFTPLNLKPHELPTDHTHMVAC
                     IVAFHRKQVVPEAEPLWMELPPDLQETTLRLAAIVRIADGLDSNMEQTTRVIAVKGRR
                     RPVVGVAGNQDTLDHDIARAEKKADLWESCIGAPPQIVAARRRSPWRPPVRRKDSVGE
                     SACIILRGYLTQVTAAIPGIGSILSVKPRHDMRIALRRIRAGLRLYRFIWEETAYNEL
                     SRELVWFGGLLGNVRDLDITILWLDKMMTACPDDVKKGLLRLRENAARRRREKLRRLL
                     VGLRSARFSALLIRLDDWVARGTGAVHILPGAEAVLGDEIRKVLARRARGILRYVGTV
                     KESSSERQHALRRECRRMRYAVDAWYRVLGKDRSDVLRALVNVQDALGDVHDADVRLS
                     VWRESERNVAVKWLRKRCQLERMKAWKAFKNTWPELQKKLDERVIESLANG"
     gene            complement(5743..7095)
                     /locus_tag="PLT86_08620"
     CDS             complement(5743..7095)
                     /locus_tag="PLT86_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT36714.1"
                     /translation="MAAEEKRPVLGIDLGGTKVLFGVVDKNGRVLTRLKVKSATTTSE
                     EIVTQLVVGIEEIRRKSDKEIAAVGIGVPGTVDTGSGHVVATANLPLNDVPLGKRLTE
                     LCGLPVVVGNDVTLCTLGEAWYGAGAEVDDLFGIFIGTGIGGGIIIDGKLYAGAHGGS
                     GEVGHIRVAQEGPVCGCGGTGCLESFASRTAIERDIRKAVEAGTATVLTDAVRSGERL
                     RSGMLRSALQEGDKLVSGILAKAAELIGLGIANVVNVLDPEVVVVGGGLIEACGDFMM
                     PIIVDSAKPRMMKVYGEPASIVRSYLGDDAGIVGAAVIAKEALKARRPAGATAYVPQV
                     EWLGAGEVQINGKRYTQDVLIRADGKVRKRPKKPAKRSSSGGNVLSIEEANAAIKGNA
                     RTLLVGTSTEHGLSVPEDVRSWLTKHDIRLLVAPGPQAVIEFKKLRGPRALLLHLRNQ
                     "
     gene            complement(7137..8216)
                     /gene="ychF"
                     /locus_tag="PLT86_08625"
     CDS             complement(7137..8216)
                     /gene="ychF"
                     /locus_tag="PLT86_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT36715.1"
                     /translation="MELGITGLPLTGKTTLFNALTGEQRATGGFSSDKPSHLAVVKVP
                     DERLDVLTAMFRPKKTVPADVKYVDVAGIVKGSAKDSRSGVLNALRTVDALIHVVRAF
                     ESDSVPAPEGGINPVRDVGDLDLEMVLADLEVAERRLDRLEKELKAGRKEGEREWTLL
                     RSCRDALESEMPLRELNLTDEEEKLLRGFQFLTRKPIVVVVNVGEDQLGNESYRSTEL
                     ASLATRAKTELIVLCAEAEMQISRLDAADQPAFLEALGISEPAVRRLVKVSYRLLDLI
                     SFFTVGPDEVRAWTVRSGALAPEAAGVIHTDLERGFIRAEVITYDDLVDCGSLAHARE
                     KGLLRLEGKAYEVRDGDILNIRFSV"
     gene            complement(8457..9206)
                     /gene="surE"
                     /locus_tag="PLT86_08630"
     CDS             complement(8457..9206)
                     /gene="surE"
                     /locus_tag="PLT86_08630"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT36716.1"
                     /translation="MRVLLTNDDGYFAAGISVLRRVLSRNPDLDVVVVAPDREQSGSS
                     HALTLHRPLRFDEVEPGVYKVDGTPTDCVLLATRGGLPEPPEAVVSGINAGANMGDDV
                     TYSGTVAAAMEGTLLGLPSLAVSLVGGTHFETAAEVAAKLLHEACTKGLPPETLLNVN
                     VPDCSMDRLKGVRVTRQSKRTYHDEVIAKRDPRGRAYFWIAGDTPTWVASDDSDYAAV
                     MSGFVSVTPLHLDLTHHAAMEALADWNLRLP"
     gene            complement(9206..>10199)
                     /gene="murB"
                     /locus_tag="PLT86_08635"
     CDS             complement(9206..>10199)
                     /gene="murB"
                     /locus_tag="PLT86_08635"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT36717.1"
                     /translation="KALLDDVCVALNREGVAAERNIPLAAYTTFRIGGVADILATATT
                     EAQIAAAVRVARKFDTGLRCIGGGSNLLVSDDGVDGIVLVNAIRRLSFEGGSATVGAG
                     YEWDSLVEASVSRGLGGIAGMSGIPGTVGGAVCGNAGAYGESVGDRVISVSVVDREGQ
                     LREIEGAGLGFCYRGSALKMSGEPIVRVRLALRPEDRRALSELRQSVLASRAEKHPPK
                     TALTAGSFFKNIDDRGERARLAIALDLATHERRLAAGLLLDRAGARGLRVGDAEVYSK
                     HANIIVNRGHATAAEVNRLAVLMKEKVRHAFGVRLEREVVWLGRGNPDGDPTGE"
BASE COUNT         2073 a         3320 c         2644 g         2162 t
ORIGIN      
        1 tcgtaacgat tcgtcggggc tcgggtcgct caatgctcaa aatccgatct atcaacacat
       61 gcgctgaagt gttccccttc gaacaccttc acctccgcga gcctgcaacc ggcgaggcgt
      121 gcgaagtgcg gttcaatctg cccccagatc cacctcgcaa gttcctcagc cgtactcgga
      181 tggctccgaa agtaaggttc gtcatcaatc atgtgatggt ccaaacgctt aatcacatga
      241 tccctcacaa tttcgtcaac tctttcgaag ttgagaaaca tgccgtgttc cagcttcgac
      301 ccttcaagag tcacttcgag gcggtacgta tgaccgtgga ttgtctggca ggggccgtag
      361 agcgcagcgt tttcctcatt ggaacaaccg gcgtcgtgca gacgatgtgc agactcgaac
      421 gaatagtacc ctgtgatacg catctctcgg tatcctgatg aaacacggga taacccgtcg
      481 tcagccgttt gggtagcccg tcataaaatc acggcccaca cgccctggca actacatcct
      541 tgagcagatg ccggtatttt gcgtaagact gataggagac gccgcgccgg acacggccat
      601 cgagcagagg aagatacctt ccctgctcaa gaagcggcac cacatcgagc tcaagccggc
      661 gctccatctc gttctcgttg agcgacaaaa tggcagagtc aatacccccg atcaacccga
      721 gcgcgccccc gtaccggttt cgtatacgcg gataggacac cccggcgacc ccgctttcac
      781 ccgaccagaa aatcgaagcg ccaaggtctc tgaacgcagg tatcagagaa gcagtgttcc
      841 cgtatgtctg gaacacaacg tgtgagacgc cggccttccg cgcctgatca agcaacactt
      901 tgtacgccgc cccgcatact ccgcgcagca tttcggggga aatgacgggc gcgcaattgg
      961 aggcgatcgg ttcggaaaac accacgaaat cgaacttcac agagtcgtac gcacgctgga
     1021 gcacctgcat cgcgaattcg gctgcttcgc gcaaccggtg ggacatcgtt tcaggttcgt
     1081 catgcagggc gatcaggagt ggcgcgagag aattccagtc cgcgacaccg aaggtcagaa
     1141 acaatccccg cgatacacgc aaaccaaccg cgaaatcacg ggtggaaaaa gagacaacct
     1201 gctggtccca gtcgcggggg tacctccgga tatcgtcggc gggacaggtt tgcacaggct
     1261 cgggattggt taccggtgtg aacgagaaat cgacgtgccg caagtcgggc tgaacaacct
     1321 caaatcgttc gatccccagt gtgcgttccc aatccgcgtc aggacacaca ccttgcgatg
     1381 cccagtcttc gaccacgtca tcccggatgc cgtcccccca aagcgggggc gagcaccggc
     1441 tcgttccggc aaacgttgca acgaaacgct ctctgctggt caggtccacc gctcgagctt
     1501 ttttctgcgg tctcggagct tctccttcac cacttttctg tgcttgtcgc tatcttcgtc
     1561 catcttcacc gcactctcga gaagaacttg aattgccttc agatgcttga caatcttccc
     1621 tcttctcgtt ccgtcgattg gcggtgttga gaccgggagc gcgtccacaa cagccccgag
     1681 cttttcgaga tccttcatga actttggctt aagcccgtat ttctgcggtt gcgcgagcag
     1741 gtcgctgaca agcaccataa tggaaccctg gtgttccgtc acggtctcct gccactgcat
     1801 ctgcccttcc gtcaggtccg aggcctgcgc gtccgtgctg gcagttgcgt cagacccgat
     1861 ttcctgcccg gtcagaacct ctttgatccg cacggcgagc aatcgtccga taccgcgaac
     1921 gtgcatcagc ttctccagcg gaacgtcccg cgccgcttga aggtccttga tacccgcggc
     1981 tttcagccat ttcgcctttg tcgggccaac cccggggagg tcgtcgagag tcttcaccac
     2041 cgccccacca gcagatgcct tcgtttcgtc caccatgtcc ccctccgctt gtcaaacttt
     2101 attcgtcgac agtcggcgca acaacatccg ccccggccga ctggctgagc gttgcagaaa
     2161 tggcagcgcg cagttcacgc tggatttcgt ccaccttctt ccgcgcgtct ataacggtga
     2221 acccgtactc gtctgacatc ttgtcgaatt ctcggataac gtccgactgg tatttgatga
     2281 aactctcata cggatcaagc tggggatact gatcgaggcc tgcttcccag tggtcgaggc
     2341 tgtctgccag caacacgcga tgaatgagcg tgttgacgtc cacacgcagg tataccacga
     2401 gatccggctc gatggctatg ccgtacaggc gccgaatcca ttccgggtca gtgccccggg
     2461 ccagcgcgcg ggcaaacgcg gtgtacacgt aacggtccgc cagaacgacg aaaccactcc
     2521 gcaaagcagg gatgatttcg tgctccaacc tgtcagcaaa atccgtcgca tacagcaaat
     2581 tgaacgtgag caggttcagc gcgtttcccg ctttcgcaag atctatgctt tccctgacca
     2641 gtgccgagcg agtccaaccg gtctcgatca ctccgtaccc ttgaacctcc agccacgtgc
     2701 gcaggaggga gagttgtgtc gtccggccca cgccgtccga gccttcaacc acgagcaggt
     2761 gcccgttgac ttctgaaagg tcaacgtatg gcagtcccgt tcccatgaac cgtcgttttg
     2821 ccattgcgtc cttccttttt cacccgtcgt tctgggtacc ggctctgcga accgtcgagt
     2881 cctttgattc gttcccggtt ttctgttcct tcgccgccag atacggagcg agatacgact
     2941 gaagctcccg cgagacaagc gccctgaact gcttctgctg agcttcgatc ggttgaacgg
     3001 catcaatcac atggaaaccg aattcttccg acatgcgatc atattcgtcg agaacacggc
     3061 tctggaacag ctcgaagcac tcgaccggat ccttgctcaa ccccgtgtcc atgcctgctt
     3121 catagaattt gagcttcatg cgcgcgctca gaaggcgtct gagggaaacg cggatgggca
     3181 ctttgaaata caccgcaaga tcaggcttta tcgcgaaatc gtacagagat cggacccatt
     3241 cgggatgaac tccccgcgca gtatcccgcg caaaggccgt gaacgcgtac cgatcggcaa
     3301 tcacaaccat ccctgcctgg agcgggggta tgatcttgta agaaagccgg tcggcaaaat
     3361 cggtaacgtg aagcaggctg aacgtagtag gggtaagcgt attgtccttc ttcgccttct
     3421 ttgtggagct tttaacaaga gctgacgaat tccattccgt ccagaacacc ggcacgccaa
     3481 gggataccag ccattgccgg agcagcgacg cttgcgtcgt ctttcccgaa ccatcgatac
     3541 cttcgacgat gatcaagcgt ccgggaaacg tgtgcttttc atggagtcgt gccattttcg
     3601 ccttctcttt cgggaaaacc gccggaggag accacgatga atttcacagg cacaccgaga
     3661 agctcctgga gaacgtcgct ttttctccct gcggcccgta attcctcatc cactcccccg
     3721 accgccttgc acgaaatagt gcagtcgttc cgcgataccc gcatctgggc ggacgtgata
     3781 agtccgcgat gtgttctgtc aaacccgtcc gcgattctga gcacaccggc gagacgacga
     3841 acggactccc gatcgtcgtc gccgagacca tcaaataccg gatcgtccgc atccgggaaa
     3901 cgctttcggt gcgcacgcgc cactgccgca atgatctgtt tttcgctccg tcggaatccg
     3961 atcagatcgg ccctgcagat catcgcctcc gaaactttgt ggtgcctgcg aacgccatta
     4021 atataaccta tatcgtgtaa tagagacgcg tactcaagcc attcacgctc ccattcccgg
     4081 tttttttttg gcggcggcag acctccgtac aactcgaggg cgagtttcgt cacctgcacc
     4141 gcatgcgctt cctcaaagtc ccacgatctg gccagctcga ttacggccgc tttcctggcg
     4201 ctccgaggaa tagatgccga ttccaagccc ttttcaccca ttcgcaaggg actcaataac
     4261 ccgctcatcg agtttcttct ggagctccgg ccacgtgttt ttgaaagcct tccatgcttt
     4321 catccgttcc agttggcacc tcttccggag ccatttcacc gcaacgttcc tctccgattc
     4381 ccgccagaca ctgaggcgca catccgcgtc atgcacgtcc cccaacgcgt cctgcacgtt
     4441 caccagcgcg cggagcacgt cgctccggtc cttgcccaga accctgtacc acgcgtccac
     4501 tgcataccgc atacgcctgc actcccgccg cagcgcgtgc tgtcgttccg acgacgactc
     4561 cttcacggtg cccacgtagc gcaatatccc ccgtgcgcgt cgcgccagca ctttgcggat
     4621 ttcgtctccc agaactgcct ccgcaccagg aagaatgtgc actgcgccgg ttccccgggc
     4681 cacccagtcg tccagacgaa tcaaaagggc actgaaacga gcgcttctga gaccaaccag
     4741 aagccttctg agcttttccc gccttcttcg cgcggcattt tcgcgcaacc tgagcaaccc
     4801 ttttttcacg tcatccgggc atgccgtcat catcttgtca agccaaagaa ttgtgatatc
     4861 caggtcgcgg acatttccaa gcaatccgcc gaaccacacc agttctcgtg aaagctcgtt
     4921 gtacgcggtt tcctcccaga tgaatcggta aagccgcaaa ccggcgcgga ttcgccggag
     4981 cgcaattcgc atgtcatgcc gcggcttcac cgacaggatt gaccctatgc ccggtatcgc
     5041 ggccgttacc tgggtgaggt atccccgcag tatgatacac gccgactccc ccactgagtc
     5101 cttccgccgg acggggggac gccaggggct gcggcggcgg gcagcaacaa tttgcggtgg
     5161 cgccccgatg cacgattccc acagatcggc tttcttttcc gcacgcgcaa tgtcgtgatc
     5221 aagcgtgtcc tgattacctg caacaccaac tactggccgt cttcggccct ttactgcgat
     5281 cacacgggtg gtctgctcca tgttggaatc caacccgtcg gcgatccgca cgatcgcagc
     5341 cagtctcagt gttgtctcct gcagatccgg gggcaattcc atccagaggg gttctgcctc
     5401 gggaacaacc tgcttccgat gaaatgcaac gatgcaggcg accatatgtg tgtggtcagt
     5461 cggcagttcg tggggtttga gattcagggg agtgaagccg cgaagtcctt cgttcgcaac
     5521 gatatcgcgc ccgcgcagat gatggttttc aggatcctcg aagaagccca cattgtgaag
     5581 cagcgctccc gcctgaaggg caggaaggta gagaggactc accttgtgcg ctggcagagt
     5641 accgagaaaa atcgtcgttg caaggcgcgc cacgtggtca ctgtgcgacg tgtcgtcgtg
     5701 gtgccttgcg cgaagggcaa taagagaagc tcggtgcatt cgctactgat tccgcagatg
     5761 taacagcagc gctctcggcc cccgcagttt tttgaactca atcaccgcct gtggcccggg
     5821 agcaacgagc agcctgatgt catgctttgt aagccacgac cgcacatctt ccggcacaga
     5881 gagcccgtgc tcggtgctcg ttcccaccaa cagagtgcgc gcgttgcctt tgatcgcggc
     5941 gtttgcctcc tcgatactga gcacgttccc gccggacgaa gagcgttttg caggtttttt
     6001 cggccttttc ctcaccttgc cgtctgcgcg tatcagcacg tcctgtgtgt agcgtttgcc
     6061 gttgatctgc acttcacccg cgccaagcca ctcgacctgg ggaacgtacg cggttgctcc
     6121 tgcggggcgc cgcgctttca gcgcctcttt ggctatcact gccgcgccaa cgataccggc
     6181 atcatctcca aggtaagaac gcacgatgga tgccggctca ccgtacactt tcatcatccg
     6241 gggtttcgcc gagtccacga ttatcggcat catgaaatcc ccgcacgcct cgatgaggcc
     6301 cccaccaacc acaacgacct caggatccag cacattcacc acattcgcga ttccgaggcc
     6361 gatgagttcc gcggccttcg cgaggatgcc agagaccagc ttgtcccctt cctgcagcgc
     6421 cgatctcagc atgccgctcc gcagtcgctc ccccgacctt actgcgtctg taagaaccgt
     6481 cgccgtgccg gcctccaccg cctttctgat gtcccgttct atcgcagtcc ggctggcaaa
     6541 cgattcgaga caacccgttc cgccgcagcc acaaaccggg ccttcctgtg cgacgcggat
     6601 gtgtccgact tcccccgatc cgccgtgggc accggcgtac agctttccat cgatgatgat
     6661 tcctccgcca atccccgttc cgatgaatat tccgaacaaa tcgtccacct cggcgcccgc
     6721 cccgtaccag gcttcgccga gcgtgcagag cgtcacgtca ttgccaacca ccaccggaag
     6781 gccgcacagt tccgtcagac gcttacccag cggcacgtca ttcagcggaa ggtttgccgt
     6841 ggcaaccaca tggccactcc ccgtgtccac tgttccggga acccctatcc cgactgccgc
     6901 aatctccttg tcactcttgc ggcggatttc ttcgataccg acaaccagtt gcgttacgat
     6961 ctcctcagat gtcgtggttg cggatttcac tttcaaacgt gtgagaacac gtccgttctt
     7021 gtccaccact ccgaacagga ctttcgttcc gccgaggtct atgccgagaa ccggacgctt
     7081 ttcttccgcc gccatcgcag gttcctccct gagaaacaca cgcctctcag caaaagtcag
     7141 acgctgaaac gaatgttgag gatatcgccg tccctgacct cgtatgcctt cccctcaagg
     7201 cggagcaaac ctttctcgcg agcatgtgcc agtgatccgc aatccacgag atcgtcgtac
     7261 gtaataacct ccgcccggat gaatccccgt tcaagatccg tgtggatcac acccgccgct
     7321 tcgggtgcca gagcccctga ccttactgtc catgcacgga cttcgtcggg tccaacggtg
     7381 aaaaagctta tgagatcgag aagtcggtac gacactttca ccagcctcct caccgccggt
     7441 tcagaaatgc ccagcgcctc caggaaagcc ggttgatcag ccgcatccag tcggctgatc
     7501 tgcatctccg cttccgcgca gagcacaatc aattccgttt tcgcgcgcgt ggcgagggaa
     7561 gcgagttccg tcgaacggta gctctcgttt cccagctggt cttcgcccac attcacgacc
     7621 accacaatgg gtttcctggt gagaaattga aaccctcgca gcagcttttc ctcttcatcc
     7681 gtaagattca gttcgcgaag cggcatctcc gattcgagcg catctcggca ggaacgaagc
     7741 aatgtccact cgcgctcccc ttctttcctt cccgccttca gctctttctc caggcggtca
     7801 agcctgcgtt cggcaacctc cagatctgcc agcaccattt cgagatccag gtcgcccacg
     7861 tctcgaaccg gattgatccc gccttccggg gctggtacgg agtcagattc gaaagcccga
     7921 acgacatgta tcagtgcatc aactgttcgc agagcgttca gcacgccact tctggaatct
     7981 ttcgcagaac ctttgacaat ccccgcaacg tcaacatact tcacgtccgc gggtaccgtc
     8041 tttttcggac gaaacatggc ggtaagaacg tcaagccgtt catccgggac cttcacgacc
     8101 gcaaggtgcg agggtttgtc ggacgagaac cctcccgttg cacgctgttc gcccgtaagc
     8161 gcattgaaaa gcgttgtttt ccctgtcaag ggcaatccgg taataccgag ttccatctcg
     8221 ccttctcttt cggtaacgtt ccccggccgg aaaagagcat cctcacaact cggtcggggc
     8281 agatttctac agaatctacc ggactcggaa cgggtttcca acagaacttg gtatgcgggg
     8341 cacgttggcg gaggtattcc cgctcaggcc tgtcggtcag acggggtccc gttcgcggta
     8401 tcctggaact cgagcggaat ccgccttgct gtctggaggt cgaaacggcc cggaggtcag
     8461 ggaagcctaa ggttccagtc ggcgagggct tccatcgccg cgtggtgcgt caaatccaga
     8521 tggagcggcg tcaccgatac aaaacccgac attacggcgg cgtaatcgct gtcgtcgctt
     8581 gcaacccagg tgggagtgtc ccctgcgatc cagaaataag cccggccccg tgggtcccgt
     8641 ttcgcgatca cttcatcatg gtaggttctt ttcgattgcc gcgtcacccg cacgcccttg
     8701 aggcggtcca tggagcagtc gggcacattg acattcagca acgtttcagg aggaaggcct
     8761 ttcgtgcacg cctcgtgcag cagtttggct gcaacctccg ctgccgtttc gaaatgcgtt
     8821 ccgccaacga ggctcacggc cagagaaggt aagccgagaa gtgtcccctc catcgccgct
     8881 gcgaccgttc cagaatacgt tacatcgtcc cccatattgg caccggcgtt gataccggat
     8941 acgacagctt cgggtggctc tggaagtccg ccgcgggtgg ccagaagtac gcaatcagtg
     9001 ggggttccgt caactttgta cacgcccggt tcaacctcgt cgaacctcaa aggacgatgc
     9061 aaagtgagtg catggcttga tccgctttgc tcacggtcgg gcgccacaac cacaacatcc
     9121 agatcagggt tgcgcgagag tacgcgacgc agcacactga tccccgccgc aaaatacccg
     9181 tcatcattcg tcaacagaac ccgcatcatt ctcccgtcgg gtcgccgtca ggatttccac
     9241 gacccagcca gacgacctct cgttcgagcc gcacaccgaa cgcatgccgg accttctcct
     9301 tcatgagtac cgccaaccgg ttcacttcgg cggcggtcgc atgcccgcga ttcactatga
     9361 tgttcgcatg tttcgaatac acttcggcat ccccgacgcg aagtccgcgg gcacccgccc
     9421 gatcgagaag aagacccgcc gccagtcttc gttcgtgtgt ggccaggtcc agtgcaatcg
     9481 caaggcgcgc tcgctcgccc cggtcgtcaa tgtttttgaa gaaggaaccc gccgtcagtg
     9541 cggtttttgg aggatgtttc tccgcgcggg aagcgagaac cgactgccgc agctcggaaa
     9601 gggcccgacg gtcctccggc cggagcgcca gccggacacg aacgataggt tctccgctca
     9661 ttttgagcgc gctccctcgg taacagaatc caaggcccgc tccttcgatt tcgcgaagtt
     9721 gcccctcccg atctaccacg ctgacagaaa taactctgtc ccccaccgat tcaccgtagg
     9781 cacccgcatt tccgcagacc gctccgccga ccgtacccgg tattccagac attccggcaa
     9841 tgccgcccaa cccgcgagaa acggatgcct ccacgaggga atcccattcg tacccggcgc
     9901 caacagtcgc agacccacct tcaaacgaca gtctgcggat ggcgttcacc agcacaatac
     9961 cgtccacacc gtcatcggaa accaggaggt tactcccccc tcctatgcac cggagccctg
    10021 tatcgaactt tcgcgcgacg cgaacggccg cggcaatctg ggcttcggtg gtcgccgtcg
    10081 ccagaatgtc cgcgacacca ccgatcctga acgtggtgta cgccgccagc gggatgtttc
    10141 gctccgccgc aacaccctcg cggttgagtg cgacgcagac atcgtcaagg agagccttc
//
LOCUS       DAOSOO010000106        10129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6636_length_10129_cov_5.630336, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000106 DAOSOO010000000
VERSION     DAOSOO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_10129_cov_5.630336"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PLT86_08640"
     CDS             complement(<1..1418)
                     /locus_tag="PLT86_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT36718.1"
                     /translation="MTPRAAIILSATLGFLTLAPSPYAATATIRVTVSPGRTDWPAAP
                     VRVPVDSLLRARGWTAPVSSSRVVVREAGRNGAVVPHRAAWGYQGVDEGTILFAKTGT
                     GTKTYEITVSDADPADAIRRLPVVGLGEPLTYGHTGVTGPMHGSFCAHAEPVDWDGDG
                     DTDLLVEEGQGGGATWTMHGMYYFENIGTPRAPLLAPARWVPVNRDPPCVVDWNNDGL
                     LDIVAGKVLHLNRGPRGAFNFAAPESLTATPGVEAMADWDGDGLFDIFCTSSRNGEEP
                     SRSVWDKKTGFIPHTPEGVWRGFTPVSSIHVYRNTGRPGAPRFEAPPELVTANSVPIE
                     LQFGIHIDLDDVDNDGDLDLIAGNNFDVYFFENVGTRTAPRLAQGFPLPLDQREILIW
                     PRFADINGDGKRDIVMAIEYGDVRWFENLGRRDGAVAFGPMRRFSLREPFIDDGCDGA
                     FDLADVNRDGKPDIIMGNANGET"
     gene            complement(1776..3542)
                     /locus_tag="PLT86_08645"
     CDS             complement(1776..3542)
                     /locus_tag="PLT86_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT36719.1"
                     /translation="MRRFLSLFCALVFATSAFATSVQSYNRPSWLHLPDNVPMVERQW
                     TNNSNKFTFVILGDRWSGGDGEENQATFDLAVREINMLRPDFVITIGDMIPGHMRGRE
                     QWHQEWADYFRPASRLGVPLLFTVGNHDISNLEMYEWWKQDRGRTYYSFDYKGAHFLV
                     INTEEERVDGRGPVWEAQMQFIERDLAENVAAPHTFIFMHKPMWIDNRYEADWARIER
                     ALGGRPRSVIAGHWHRVQTDWRNGNLYAVFGGTGCGGRNPVLELGAFQHYTHVTVDGD
                     SASFALIQPGKGIVSVDVPSRAFQDGLAGMVRTLSGGLQVARDGAATLSARVSIANPL
                     PDSTSVTVRAVLPPGTAWTKASGVDSFTVSLAPGQRAERVFTFAGTTTQLSPVPVFVS
                     NVDYRGVAAAPRRPVPVRLYPDSLMRAIREWMVIGAFELGPFNTRLLPDNPQEALPGL
                     FARREPRNFFDARRPVVENGTEFRWRKVTADATGYMNFNAYLGTKDQALAYAMTKVYS
                     PVAQEVYAEFAADNYAQVVLNGEIVGQKFANPGSPGITPLRLKKGWNTLVLKLINNSG
                     DWYLEFALADPRQNLRFSVDGR"
     gene            complement(3844..4719)
                     /locus_tag="PLT86_08650"
     CDS             complement(3844..4719)
                     /locus_tag="PLT86_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36720.1"
                     /translation="MQGIRKLGKVQDNLVEVSPFVFRGELLLFESVRPDTPDNTRGGR
                     HYLRIRRLPEGRRDVTGPEEFSACPVLTEFGEEYTFGVPFVWNDEIFIYASLGKKPTV
                     DDIHVFHSRTMDNWQHSIAVRGADEQVFNSSVCRAGDRFIMAYESNFSAEWVPFTNRF
                     AESKDLVHWTPISPDRAVHGSDRYCACPTLRHIDGTFVIVCLEMPHRGAWWFEEFAAT
                     STDLLNWTMSPANPILAPPDDRSENINNSDIDFCGFGDKTIIYYSWGSQRGDEHLAHA
                     VYDGPEEAFLRAMIG"
     gene            complement(4751..7054)
                     /locus_tag="PLT86_08655"
     CDS             complement(4751..7054)
                     /locus_tag="PLT86_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36721.1"
                     /translation="MITANPEPQRSLKTETLECDLAVVGGGLAGCCAALTAARAGLKV
                     VLMQDRPVLGGNASSEVRLWGLGATCHMGSNNRWARESGVIDEILVENHFRNPEGNAV
                     IFDTVLLEWVLREPNITLLLNTAAFEVAKSDDSTIARVKGFCAQNSVLYDVGAKLFCD
                     ASGDGILGFLSGAAFRMGAEAKEEFNEPLAPSIEYGELLGHSIYFMSKDVGVPVKFVA
                     PSYALKDIKKIPRFKNITAEAGSHKCHLWWFEYGGRLDTVHDTEKIKWELWKVAYGAW
                     DYIKNSGKFPEAANLTLEWMGCIPGKRESRRFEGDYMLTQGDIIHQHHHEDAVSYGGW
                     SIDLHPADGVYSPLPGCNQWHARGVYEIPYRTMYSRNIANLFLAGRLVSSSHVAFGST
                     RVMLTCAESAQAVGMAAALCVRDKLLPKDLAEPKRMKELQTRLLQAGQYIPAVPHNDV
                     DDLAQSATITATSSLKLSRLDPDGTTKQLTKGQGLLLPLLPGPAPKVTLFADAARPTK
                     VRVELRTSDRPENHTPDVVLGTQEISLPAGENQPLTLAFGATITEPRYALYCVRANEA
                     VSLRCSGERVTGVMAVAHRFTHQHDAALDVETYEVWAPERDPLRRTFACQIDPPLAAF
                     SPQCVANGFARPTCRPNAWVADFSDDDPVLAIAWGIPQTISHIQITFDTDWEHPLESV
                     LWPHHSGLIPSCVKSFRIFGDMGALLYEEKNNHQTRVMVALPKPVRASRITIECVANW
                     AEEMGIKVPPAAIYDVQVYGSDPGRIV"
     gene            7488..>10129
                     /locus_tag="PLT86_08660"
     CDS             7488..>10129
                     /locus_tag="PLT86_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36722.1"
                     /translation="MRATVFAVLFMVANGSTAMDVLVRPPFDAASPALVYASSRVQAA
                     AERAGFARGAPRVTVEMAATGALRPESFTMAPSATDSGRPEDRRTVTITAADARGAAW
                     GLLWLADRITVEGRDAFTRRWERTPAFRRRFLLDGASFRTPPGSPPEATLELDLRFTC
                     NTHAHSFNTSGAAGNVYLDEIDPRLVDDVAAHHREVDANRDAMRQELALLRSYGIDSY
                     RDCSPFSDLGFMANRMIELYGDEVSEDGVTLSPALDRPWEIWAAMQEAYLAAFPDIAG
                     IRSSMGDFSEQYQIWRAHGPRSAAIGPAGGMRRFLEYARDAVVRKHGRELIVSAWGNP
                     PEEYPLNQPAALRRIFADVSPEDPISLLSNECEHDFYLNSPFNDNFAAIDIRRGIMFQ
                     VQREYQGQGDLPVYVGRRIREHLGRAAGYGATDMAAARLWWSKNLYNEGVCWTWWNMY
                     AWQRATWEPNGDPWEWARDYSTIAFGRQGARELADALMLTEELAARLFTIPGFTGGKR
                     DAYTLTHRNVITDGRHYWRRTPDPQGEGFREHHLGGRLTELLVSVDATERLADTLFAK
                     SRVARAAMGDSEKARAVMSSFEHLHTLAPVLTHYQRAILLWHGKDAPQLTPAMREEAR
                     GRCVKECYATLAAFGEYRARYDMYRDGGMVPLVRSWLRALGEPLPPEIEVPPQFPIAR
                     SARLLPVDGTLRGWPAPAIVMDTSNAEVFGNAGAFRAEIRLAWDADALKVAVDAPGGW
                     PAEGIRAGRLYYSDSVSLSFDTDNDGREDAIYFLLIDGASGEPRLHTLMRRMVFYSSN
                     YEERGAEPPPDPGASVKLVKTDSGRRWVAAIPISMLDGYRPDSGVPLGVRVQVNVNSA
                     PGLGAHRVAWPRNPAWRDAPPEWMG"
BASE COUNT         1775 a         3003 c         3480 g         1871 t
ORIGIN      
        1 gtctcgccgt tggcgttgcc catgatgatg tcgggcttgc cgtcgcggtt cacgtcggcg
       61 aggtcgaacg ccccgtcgca cccgtcgtca atgaacggtt cccgaagcga aaaccggcgc
      121 atggggccga aggcgaccgc gccatcgcgc cggccgaggt tttcgaacca gcgcacgtca
      181 ccgtactcga tcgccatgac gatatcacgt tttccgtcgc catttatatc ggcaaaacgc
      241 ggccagatga ggatttcgcg ctggtcgagg gggagcggga agccctgcgc aagcctcggc
      301 gcggtgcggg tgcccacatt ctcgaagaag tagacgtcga agttgttgcc ggcgatgaga
      361 tcgagatcgc cgtcgttatc cacgtcatcg aggtcgatgt ggatgccgaa ctggagttct
      421 atcggcacac tgttggccgt gaccagttcc ggtggtgcct cgaaccgggg tgcgccgggg
      481 cggccggtgt tgcggtagac atgaatcgaa cttaccggcg tgaacccgcg ccagacccct
      541 tcgggtgtgt gggggatgaa cccggttttc ttgtcccaga ccgaccgact cggttcctcc
      601 ccgttgcggg aagaggtgca aaaaatgtcg aacagtccgt cgccatccca gtccgccatc
      661 gcttccacgc cgggggtcgc ggtgagggac tccggcgccg cgaagttgaa tgcgccgcgc
      721 ggaccacggt tcaggtggag gaccttcccg gcgacaatgt cgagcaggcc gtcgttgttc
      781 cagtcaacca cgcacggtgg gtcgcgattc acgggcaccc atcttgccgg agcgagaagt
      841 ggtgcccgcg gggtgccgat gttctcgaag tagtacatgc cgtgcatggt ccacgtggcg
      901 ccaccgccct gaccctcttc gacgaggaga tcggtgtcgc cgtcaccgtc ccagtcgacg
      961 ggttccgcgt gagcacagaa gctgccgtgc atgggtcccg tgacaccggt gtggccgtag
     1021 gtcagcggtt cacccagtcc caccacgggg aggcggcgga tggcgtccgc ggggtcagcg
     1081 tcggagacgg tgatctcgta cgttttcgtc cccgtgccgg ttttggcgaa gaggatggtg
     1141 ccttcgtcca cgccctggta tccccaggcg gcgcggtgcg ggaccacagc gccattccgg
     1201 cccgcctcgc gcacaacgac gcggctcgag gacacgggcg ccgtccagcc gcgcgcgcgc
     1261 agcaggctat ccaccggcac gcgcaccggt gcggcgggcc agtcggtgcg gccgggagat
     1321 acggttaccc ggattgtcgc ggtggcggcg tagggggatg gcgcgagcgt gaggaatccg
     1381 agtgtggccg acagaatgat ggcagcgcgg ggtgtcatgg gtatgccctc gttgtcacgg
     1441 gtcaatgcgt cggaagaaag gtagggggat ggaggcgatg cggcaacgcg gtgaacaacg
     1501 cacaggtgca tggtggcaaa agcccgaccc gggcaggcag gaatgcctgc cccaccagca
     1561 ggaacgcccg ccccaccaaa agaatccgga gtcaactgtg gagcaggcgt gtcattcccg
     1621 cgcaggcggg aatccagaac aggcgccccg gcatcgggca gtcaggcagc gcaatgcagc
     1681 cgcaaatgtc gtacaggacc tgtggaacgg cgtggcggcg cggtggttga gtgtgcgtgc
     1741 cgcggcctca accaccgggt gccacagtgc cctttctacc tcccgtccac cgagaaacga
     1801 agattctgcc ggggatcggc cagggcgaat tccaggtacc agtcgccgct gttgttaatc
     1861 agcttgagca cgagtgtgtt ccagcccttt ttcaagcgca agggcgtgat gccgggcgaa
     1921 ccggggttgg cgaatttctg cccgacaatc tcaccgttca ggacgacctg cgcgtaattg
     1981 tcggcggcga actccgcgta cacctcctgc gccacggggc tgtagacctt cgtcatggcg
     2041 tatgcgaggg cctgatcctt cgttccgagg tatgcgttga agttcatgta cccggttgcg
     2101 tcggcagtaa ccttccgcca gcggaattcg gtgccgtttt ctaccacggg ccggcgcgca
     2161 tcgaagaagt tgcgcggttc gcgccgcgcg aagagaccgg gcagggcttc ctgcgggtta
     2221 tccggcagga ggcgggtgtt gaacgggccc agctcgaacg cgccgatgac catccactcg
     2281 cgaatggcgc gcatcaggga gtcgggatac agcctcacgg gaaccggcct gcgaggcgcc
     2341 gccgcaacgc cacggtaatc cacgtttgag acaaacacgg gaaccgggga gagctgcgtc
     2401 gtggtgcctg caaaggtgaa caccctttcc gcgcgctgcc ccggcgcgag gcttacggtg
     2461 aaggagtcca cgccgcttgc ttttgtccat gccgtgccgg ggggaagcac ggcacgcacg
     2521 gtgacggagg tggaatccgg gagcgggttg gcaatggaga ccctggcgct cagtgttgcc
     2581 gcgccgtctc gtgcaacctg caatcctccg gacagcgtac gcaccatgcc ggcgaggccg
     2641 tcctgaaatg cacgcgacgg gacgtcaacc gacacgatgc cttttcccgg ctggatgagc
     2701 gcgaaggaag cggagtcgcc atccaccgtc acgtgggtgt agtgctggaa agcgccgagc
     2761 tcaagcactg ggtttcgccc gccgcaaccc gttccgccga acacggcgta gaggttaccg
     2821 ttgcgccagt cggtctgcac gcggtgccag tggccggcga tgacggaacg cgggcggccg
     2881 ccaagcgcgc gttcgattcg cgcccagtcg gcctcgtagc ggttgtcgat ccacatgggc
     2941 ttgtgcatga agatgaacgt gtggggcgcg gcgacgtttt cggcgaggtc gcgctcgatg
     3001 aattgcatct gggcttccca cacgggtccg cggccgtcca cgcgctcttc ttccgtgttg
     3061 atcaccagga aatgggcacc cttgtagtcg aaggagtagt aggtgcggcc acgatcctgt
     3121 ttccaccact cgtacatctc gaggttggag atatcgtggt tacccacggt gaaaaggagc
     3181 ggcacgccga gcctgcttgc cggacggaag taatcggccc attcctgatg ccattgctcg
     3241 cggccgcgca tgtgcccggg aatcatgtcg ccgatggtga tgacgaagtc gggccggagc
     3301 atgttgattt cgcgcacggc aagatcgaag gtggcctgat tctcctcgcc gtcgccgccg
     3361 ctccatcggt cgcccaggat gacgaacgtg aatttgttgc tgttgttcgt ccactggcgc
     3421 tccaccatgg ggacgttgtc cggcaggtga agccacgacg ggcggttgta cgattgaacc
     3481 gacgtggcga acgctgatgt tgcgaacacc agcgcacaga aaagggaaag gaaacgtcgc
     3541 atgaatgggt cctccgtgat cagtaaacag gtttcaggtg atgacgaagc tcgaacaggt
     3601 atcgcgcggg gaagatgccc gcgtaatgtg tgccccttca atcgcaggag attcttcgcc
     3661 tgtcgtccgc actcgcggac tccgctcaga atgacacttg cgtgtgtgtg tgtgtcaggt
     3721 gagaaacgtc atcccggcgc cagacaccgc cattctgaac gcgacaaagc gtcattctga
     3781 gcgaagcgaa gaatctccgt tgtgcacagt cagggatccc acatcccttt ggccagccgt
     3841 tgctcagccg atcatggcgc ggaggaacgc ctcctccggg ccgtcgtaga ccgcgtgggc
     3901 gaggtgctcg tcgccgcgtt ggctgcccca ggagtagtag atgattgtct tgtcgccaaa
     3961 accgcagaag tcgatatcgg agttgttgat gttttccgaa cggtcgtccg gtggcgcaag
     4021 gatcgggttg gccgggctca tggtccagtt caggaggtct gttgacgttg cggcgaactc
     4081 ctcgaaccac catgcgcccc ggtgcggcat ttcgaggcag acgatgacga acgtgccgtc
     4141 aatatggcgc agggtggggc aggcgcagta tcggtcagaa ccgtgaaccg ctcggtcggg
     4201 cgaaatcggt gtccagtgca cgagatcctt cgactcggcg aacctgttcg tgaagggcac
     4261 ccattccgcg ctgaagttgg attcgtacgc catgatgaac cggtcgcccg cccggcagac
     4321 gctgctgttg aagacctgct cgtccgcgcc gcgcactgcg atggaatgct gccagttgtc
     4381 catggtgcgg gaatggaaga cgtggatgtc gtccacggtg ggttttttgc cgagggatgc
     4441 gtagatgaaa atttcgtcgt tccagacgaa cggcacgccg aaggtgtact cctcgccgaa
     4501 ttcggtcagc accgggcacg cggagaattc ttccggcccg gttacatcgc gccggccctc
     4561 cggaaggcgg cggatacgca gatagtggcg cccgccgcgc gtattatcgg gtgtatcagg
     4621 gcggaccgat tcgaacagga ggagttcccc tctgaagaca aacggggaga cctcaacgag
     4681 gttatcctgc actttcccga gtttgcgtat tccctgcatg agagcctctt ttctgccagg
     4741 ggccagacga tcagacgatc ctgcccgggt cgcttccgta cacctgcacg tcgtaaatgg
     4801 cggcgggcgg caccttgatc cccatctcct cggcccagtt tgccacgcac tcgatggtga
     4861 tgcgcgatgc acgcacgggt ttcgggagcg caaccatcac gcgggtctgg tggttgtttt
     4921 tctcctcgta gagcaaggcg cccatatcgc cgaaaatccg gaagctcttc acgcaggagg
     4981 gaatcaatcc gctgtgatgg ggccacagga cggattccag cgggtgctcc cagtcggtgt
     5041 cgaacgtgat ctggatgtgg gaaatcgtct gcggtatgcc ccatgcgatg gcgaggaccg
     5101 ggtcgtcatc cgagaagtcg gcaacccatg cgttgggccg acaggtgggg cgggcgaagc
     5161 cgttggccac gcactgcggc gagaaggccg cgagcggcgg atctatctga caggcaaacg
     5221 tccggcggag cgggtcgcgt tccggcgccc acacttcgta ggtctcgaca tcgagcgcgg
     5281 cgtcgtgctg atgggtaaag cggtgagcca ccgccatcac gccggttacc cgctcgcccg
     5341 agcagcgcag gctcactgcc tcattggcgc ggacgcagta cagcgcgtag cgcggttcgg
     5401 tgatggtggc gccgaaggcc agcgtgagcg gctggttctc gccggcggga agagatatct
     5461 cctgcgtgcc cagaacgacg tccggcgtgt ggttctccgg acggtcgctt gtacggagtt
     5521 cgacgcgaac tttggtcggt ctggcggcgt cggcgaacag ggttaccttg ggcgccggcc
     5581 ccggcaggag cggcagcagc agcccctgtc ctttggtcaa ttgtttcgtg gtgccgtcag
     5641 ggtcgagccg ggagagcttc aggctgctgg ttgcggtgat ggtggccgac tgggcgaggt
     5701 cgtccacgtc attgtgcggc acggcgggaa tgtactgccc cgcctgcaga aggcgtgtct
     5761 gcagttcctt catgcgcttc ggttcggcga ggtctttggg gagcagtttg tcccgcacgc
     5821 acagcgccgc ggccatcccg accgcttgcg cggattcggc gcatgtcagc atcacgcggg
     5881 tggacccaaa cgcgacgtgg gaggagctga ccagccttcc cgcgaggaac aggttggcga
     5941 tgttccggct gtacatcgtc cggtaaggga tttcgtacac gccccgcgca tgccactggt
     6001 tgcaaccggg caggggagag tagacgccgt ccgcagggtg aagatcgatg gaccatccgc
     6061 cgtaggaaac tgcatcctca tggtggtgct ggtggatgat gtcgccctga gtgagcatgt
     6121 aatccccttc aaagcgccgg ctttcgcgct tgccgggaat acagcccatc cattccagcg
     6181 tcaggttggc cgcttcgggg aacttgccgg aattcttgat gtaatcccac gcaccgtagg
     6241 cgaccttcca gagttcccac ttgatctttt cggtgtcgtg caccgtatcg agccgtccgc
     6301 cgtattcaaa ccaccagaga tggcatttgt gggagcccgc ctcggcggtg atgtttttga
     6361 accgggggat tttcttgatg tctttcaggg cgtaactcgg ggcaacgaac ttgaccggca
     6421 cgccgacgtc cttgctcatg aaatagatcg agtggccgag gagttcgccg tattcgattg
     6481 agggggcgag cggctcgttg aactcctcct tcgcctcggc gcccatgcgg aaggccgcgc
     6541 cggaaaggaa gccgaggatg ccgtcgcctg atgcgtcgca gaagagcttc gcgccgacgt
     6601 cgtacaggac ggagttctga gcgcagaagc cttttacgcg ggcgatggtg ctgtcgtcgg
     6661 atttggcgac ctcgaacgcg gcggtgttga gcagaagggt gatgttcggc tcccgaagca
     6721 cccattccag aagcacggtg tcgaagataa cggcgttgcc ttcggggttg cggaagtggt
     6781 tttcgacgag gatttcgtca attaccccac tttcacgcgc ccagcggttg ttgctgccca
     6841 tgtggcaggt ggcgccgagc ccccagaggc gaacttcgct tgacgcgttg ccgccgagca
     6901 ccggccggtc ctgcatcaga acgaccttga gaccggcccg ggctgcagtc agcgcggcgc
     6961 aacagcccgc aaggccgcca cccacgacgg cgaggtcgca ctcaagcgtc tcggttttca
     7021 ggctgcgctg cggttccgga tttgcggtga tcatcgcctg atctccctca cactcgcgat
     7081 acaacggctg ccccccgcca gatcttactc atactgacaa gtgtacgccg aacggacgcg
     7141 agacggaacg ggatggtgcg cgccgaggcc aacgggtttc tgtgtggtgc ggggaaggtt
     7201 gacggaggga tggtaccggt gtggcggggc gtgtggcggc aggggcgacc ggtccgtgcg
     7261 gtgctgagcg cgcgggactg gccggcccgt accgaaaatg aacttttttg cagcttgctc
     7321 ttgacacgag cacgccgttt tgccacagcc atactggagg caggtgttcg gatggctcgc
     7381 aagggtttga ccgaagccgg gcagccggta tgcttacggc tgatgtgttg cgtcgcgcgt
     7441 ggttcccggc ggggaacgcg ctgccccgtc accttccgga gaacttcatg cgcgccacag
     7501 tgttcgctgt tttgttcatg gttgccaacg ggagtactgc catggatgtt ctggttcgtc
     7561 caccgttcga cgcggcgtcg ccggcgcttg tgtacgcctc gtcgcgcgtt caggccgcgg
     7621 cggaaagagc aggatttgcg cgtggcgccc cgcgggtgac ggttgaaatg gccgctacag
     7681 gcgccctgcg tccggaatcc ttcaccatgg cgccatcagc gaccgattcc ggacgcccgg
     7741 aagaccggcg gacggtgaca atcaccgcgg cagatgcccg gggcgcggcg tgggggcttc
     7801 tgtggctggc agaccgaatc accgtggagg ggagagatgc cttcacacgg cggtgggaac
     7861 gcacacccgc attccggcga cggtttctgc ttgacggggc atcgttccgc acgccaccgg
     7921 ggagcccgcc cgaggcgacg ctggagctgg atctccgctt cacctgcaac acccatgcgc
     7981 actcgttcaa cacgtccggc gcggcgggga atgtctacct cgatgagatt gacccgcggc
     8041 tggtggacga cgtggcggcg caccacaggg aagttgacgc caaccgggat gcgatgcgac
     8101 aggaactggc gctcctgcgt tcctacggga tagactccta ccgcgactgc agcccgtttt
     8161 cggacctcgg cttcatggcc aaccggatga ttgagctgta cggcgatgag gtctcggagg
     8221 acggcgtcac actgtcgccc gcgcttgaca ggccgtggga gatatgggcg gcaatgcagg
     8281 aggcgtatct cgcggcgttt ccggatatcg ccggtatccg gtcgtccatg ggcgatttca
     8341 gcgagcagta ccagatctgg cgggcacatg ggccccggtc ggcggcaatc ggcccggcgg
     8401 ggggcatgcg gaggtttttg gagtatgccc gggatgcggt ggtccggaag catgggcgcg
     8461 aactgatcgt ttcggcgtgg ggcaacccgc cggaggaata cccgttgaac cagccggcgg
     8521 cgttgaggag gattttcgcg gacgtatctc ccgaggaccc gatttcgctg ctttcgaacg
     8581 agtgcgagca cgacttctat ctgaattccc cgttcaacga caacttcgcc gcgatcgaca
     8641 tccgacgcgg gatcatgttt caggtgcagc gcgaatatca ggggcaaggt gacctgccgg
     8701 tgtacgtggg gcgacgcatc cgtgaacacc tcggcagggc ggctggctac ggcgcgaccg
     8761 acatggcggc ggcgcgactc tggtggagca agaacctcta caatgagggc gtgtgctgga
     8821 catggtggaa catgtacgcc tggcaacggg caacatggga gccaaatggc gacccgtggg
     8881 agtgggcgcg ggattactcc accatcgcgt tcgggaggca gggcgcgcgc gaacttgcgg
     8941 atgcgctgat gcttaccgaa gaactggcgg cgcggttgtt cacgattccc ggattcaccg
     9001 gcggcaaacg agatgcctac acgctgaccc accggaacgt catcacggac gggcggcact
     9061 actggcgccg gacgcccgat ccgcagggag aggggttccg cgagcatcat ctgggtggac
     9121 gtttgacgga acttcttgta tcggttgacg cgacggagcg gctggcggat acgctgttcg
     9181 ccaaatcgcg cgttgccagg gcggccatgg gggatagcga aaaggcacgg gcagtgatga
     9241 gcagtttcga acacctgcac acgctggccc cggttctcac ccactaccag cgggcgattc
     9301 tgctgtggca cggaaaagac gcgccgcagt tgacgccggc gatgcgggaa gaagcccggg
     9361 gccgctgcgt gaaggaatgt tacgccacgc tggccgcatt cggcgaatac cgcgcgcggt
     9421 acgacatgta ccgggatggc ggcatggtgc cgctggtgcg gtcatggttg cgcgcgctgg
     9481 gggagccgct gccgccggag attgaggtgc cgccgcagtt cccaatcgcc cgctctgcgc
     9541 gcctgcttcc cgtggacgga accctgcgcg gctggccggc gccggcgatc gtgatggata
     9601 cgagcaacgc cgaagtgttc ggcaacgcgg gagctttccg ggctgaaatc cgcctggcgt
     9661 gggatgccga tgcgctcaag gttgccgtgg atgcgccggg cggctggccg gcggagggaa
     9721 tacgcgcggg caggctctac tacagcgact cggtatcgct cagcttcgac acggacaacg
     9781 atggccggga ggacgcgatt tacttcctgc tcatcgacgg cgcgtcgggc gagccgcgcc
     9841 tccacacgct catgcggcgg atggtgttct actcgtcgaa ctatgaggaa cgcggggccg
     9901 aaccgccgcc cgaccccgga gcgagcgtga agctggtgaa aacggactcc ggcaggaggt
     9961 gggtggccgc cattccgatt tcgatgctgg atggctaccg cccggactcg ggggtgccgc
    10021 ttggcgtgcg ggtgcaggtc aatgtcaatt cggcaccggg gttgggcgca caccgggttg
    10081 cctggccgcg gaaccctgcg tggcgggatg caccgccgga gtggatggg
//
LOCUS       DAOSOO010000107        10063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6689_length_10063_cov_6.368905, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000107 DAOSOO010000000
VERSION     DAOSOO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6689_length_10063_cov_6.368905"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLT86_08665"
     CDS             <1..377
                     /locus_tag="PLT86_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36723.1"
                     /translation="ELRQRGQYAQAIEAFSEAIRLRPDFADAYNGRGVARNHEGGRVR
                     YMSDDPPRRREEAERLWRDAVADFREAIRLNPRESGYWVNLMISFQLLNDTQEAQQAR
                     EQALQIDEELRPVLEDIFRTGQ"
     gene            584..1114
                     /locus_tag="PLT86_08670"
     CDS             584..1114
                     /locus_tag="PLT86_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36724.1"
                     /translation="MRRTWCVAGMIAALGVCALSTGVHAANPDTSRSAKSAAMLMRLA
                     RAQQLSARPRTFQTQQGVAVAARRHADAMEQQKRTLAILNNQYLRGTAATSPSESDVR
                     QTIGVLEQQLNAPTPPENAYEIRYYLAACYESLDNIPKAKELLQTIVAQYGSSTDDVV
                     KGFVEQARADLARLGS"
     assembly_gap    1134..1233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1234..4572
                     /locus_tag="PLT86_08675"
     CDS             <1234..4572
                     /locus_tag="PLT86_08675"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOT36725.1"
                     /translation="HIHLWLALAAGLFFVLLSLTDFYELIELKTYDYRLILRNKIFGP
                     PHQLPNLATIDVDDYSVDKEGRFEDWDRTYHARIIDIASALGARAIGVDFLMPEPSTP
                     MIRENQVAESDVHSREAVLALFRNPDVVLSDACRKWNNVYFAQYLTEAETQDYDRSLR
                     ENPPRTEVEEHRFQLVQRFTIPITQDFQKEFVVGSQLWAPVDTFLATARGAGQVQPIP
                     DMDGIVRRNRAFYVYDGRIFPSLSIVMAADYLGVPLSSFKFEPGRVTLPNAHIPGEPA
                     PRDIVIPLGARGTILVNWAGDYRSTYRHFPYASVKTFWEVHQREQLAGLVKRDLARDP
                     ALLDGLMGGQIDLSEFTLSRMMELSRVPSVDVKMGLQDVAVALKMSGAVSRRAPVTEA
                     LRDAFGKTTGIPDIGRWRALYEELLLNGRVADLFAGRPDLTLADAARDLNMPAARLES
                     TYTKIRTLVSPLTGEIAPGNYPLWFFAQTRDGAPVAVNDSVNSVLASAMKEFLRRHGV
                     ISDAVVTGIVTPTPETGADLTNLAVRVVAFAAGYPEQDYVAVTGSIVQAALLEPLVAE
                     GTSFDDIVQQLFQVSPSDLPAEVIQPYRDTYNTLWRNNNLAAVLKADTSLSVEAAADT
                     LTVLLRKSAGNDSVAVSAVDIVAEHAILLKHLKEQGDIPPEAHPLVFNTVVVDGRPVF
                     VSDFEGAVLFYGITSTGGHDRNPTPFEPRYPMVGMHLNLFNQVVTGGFLYQAPKWLDW
                     AVILAVALLMGLLVPKMSPVNGGILAFAMVVVFLTSTVVLFAKGRIWIDTVGPFGAIT
                     FGYLVITVRNYIVGEKEKKFIKGAFSAYLAPEVVEIIANDPEGLKLGGSPEHITAFFS
                     DVKGFSTISEALSPTELVNLLNEYLTEMCDIAFKYGATVDKFIGDAIVAFFGAPIKYE
                     DHAVRACLCACEMQSRLAVLREKWKAEGKHEIYCRIGLNTGNAVIGNMGGRQRFNYTM
                     MGDTVNLAARLESGAKQFGVYTMITDVTYNAARDVIEARELDRIVVIGKKEPVTIYEV
                     LSKKGELDPKKKQIADFYAEGLAYYREQNWDAAVAKWRAAMAVDPDHPDATSGIFIKR
                     VDAIRAGKVEIPKDWNGVWEMTEK"
     gene            4648..5388
                     /locus_tag="PLT86_08680"
     CDS             4648..5388
                     /locus_tag="PLT86_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT36726.1"
                     /translation="MGFPSPVNLSVSLLVVMSSTAFASPFVGSPREYALGIGALGTAA
                     LLEQWPPNRSHPLIGGEQKPYIERERVPDWALAGAHLVTIAAIRFSDSPDRVRYAHGY
                     GMAVALTQATTSFTKGIVGRKRPNEDAAVALGKSSKSRSFFSGHASTGFCLATYSSAY
                     AWRASPKLEVRAGVPLATYGAATYAAWSRVAEHRHYPADVIAGAAAGTAIGALVFRWY
                     DGMEAGGRAGASASVFPLPRGAAVSVAF"
     gene            complement(5473..8151)
                     /locus_tag="PLT86_08685"
     CDS             complement(5473..8151)
                     /locus_tag="PLT86_08685"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOT36727.1"
                     /translation="MFHSFLRAAALSAAFLATSAHGGTVVLNEVMASNSATITDAAGT
                     YADWIEIYNDSTDAVQLGGYGLTDTPSNPFQWVFPAVSIAPGEFLLVWASGKDSTDPV
                     GALHTNFKISSDGETIVLTRPDGTLQDSLPRAAMGKDVSWGRTPDGGADLVFFAAATP
                     GRSNAGGGQAVLEPPAFSHSGGFYPAPFDLSISSSDAQATIRYTVDGSEPTETSPVYS
                     GALRIEDRTGPASGILLIRTTNPQNVWHAPTGNVFRGVPVRARAFKTGCLPSKTVTGT
                     YFVTPKGRARYPFPVLSLVTDSLNLFDDTIGIYVPGSKYAQTGLKEDANFYQSGGDWE
                     RPVHVELMEEDGTLAFSQGAGMRIHGNYTRDYFQKPFRLYAKSSYGKDWFETRLFPES
                     DITRFKRILVRTGASDIYTALIRDGVMQSLVSHLSFDTQKYRPVVVFFNGDYWGIQFF
                     RDRYDEYYLQTHHGADPSAVDLIEIDYSLPGGPYDADPGDAVAFTDMLSYTKNHSATD
                     PQVYEHFKTLVDVENFAEYCAANIFVANTDWPHKNVRVWRARVPYSPSAGQLDGRWRW
                     LAYDFDRGFGLRESSQADQNTLIWATIGNPNQESTWLLRWLLLNPDFRTMFINTLADQ
                     LNSTFTEPRMIGMIDSLAAQIAPGVPEHIARWGEPKYLYYWTAYVQTLRNFATARRPH
                     MIQHILGYFRTYTADTAAVTINTDGPAQNCGTVRINSITIEDGFPGVSHLVYPWRGVY
                     FRDVPVRLVATPKPGYVFAGWETAAGIISLSDTLTLSLTTDTTVTAKFTNPADVAAPP
                     SSRAFYLDQNTPNPFNPSTTLRFGLPDAGYVTLAVYDVNGRQVRNLVGRTFLSGHHEA
                     VWDGCDNNGRAVASGVYLVRLTVREGVLTRRVTLLR"
     gene            8386..9426
                     /locus_tag="PLT86_08690"
     CDS             8386..9426
                     /locus_tag="PLT86_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36728.1"
                     /translation="MATDRELFSPLPREEVIKAVERRKPARIPLVMAKWWGEGLGDQY
                     GDRLREFDRIPDDVGMLWWGPLDVGKMGLSWQAKSGAAHDSHCVIDDWNHLDEFIEKM
                     PDPERDDAFSGLLEHAERMRKDDRYILFGWWGLFFERPWGLRGMENLMADYYEAPEHV
                     HRLHSALADLYIGYIRRAAREFQPDGFWTSDDLGHQTQSMMRREQFDALLKPYYDRVG
                     KACKEAGMHWWLHSCGNNTVLLPSLIDAGVDVFHPVQKHTMDEIAVAREFGGKITFLA
                     GIDVQHTLQEKTPEGVREEVRFLIDTFDQPDGGMCIASGNGIVSGTPFENIEAFLDES
                     VRYGAAHRRRFA"
     gene            complement(9547..9717)
                     /locus_tag="PLT86_08695"
     CDS             complement(9547..9717)
                     /locus_tag="PLT86_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36729.1"
                     /translation="MKSSKPECKNYIAEDLCAASANHPEEHCHDDVKGDCGRFVPVQT
                     LETPSTEKKKTA"
BASE COUNT         1930 a         2833 c         3210 g         1990 t
ORIGIN      
        1 cagagcttcg ccaacgaggg cagtacgcgc aggccatcga agccttcagc gaagcgattc
       61 gcctgcggcc ggattttgcg gatgcgtaca acgggcgcgg cgtagcgcgc aaccatgagg
      121 gcgggcgtgt cagatacatg tcggatgacc cgccccggcg gcgcgaggag gcagagcgtc
      181 tgtggcgcga cgcggtggcg gatttccgtg aggcaatccg gctcaatcct cgtgaatctg
      241 gctactgggt gaacctgatg atttcgttcc agctcttgaa cgatacgcag gaagcacagc
      301 aggcacggga gcaggcactg cagattgacg aagaactccg gccggtactg gaggatattt
      361 tccggaccgg gcaatgacga ggctgtactg ctgccatgaa atgccgctcc acagggcgcg
      421 gaacaggcat cacttgcgga aaaatcctgg cgccgtatat tttctattgt gctcaacggt
      481 acacttttgt gaaggaacgc gtacacttgt cttacgggta gcgcactttt gcaaagagca
      541 ctttgatttg aacatttttc atttttttca ggaggcgtgt acgatgagac gcacgtggtg
      601 tgttgcaggg atgatagcgg ctctgggagt gtgtgctctc agcacggggg tgcatgccgc
      661 caaccctgat acctcccgaa gcgcgaagag tgcagcgatg ttgatgcgtc tggcgcgagc
      721 gcagcaactg tcggctcgtc ccaggacgtt ccagacgcag cagggagtcg cagttgccgc
      781 gcgtcgccac gccgatgcca tggagcaaca gaagcgcacg ctggcgattc tgaacaatca
      841 gtatctccgc ggcaccgccg caaccagccc ttcggagagt gacgtccgac agacgatcgg
      901 cgttctggag cagcagttga acgcaccgac gccgccggag aacgcgtacg aaattcgtta
      961 ctaccttgcg gcgtgttacg agtcgcttga taacattccc aaggcgaaag agctgcttca
     1021 gacaatagtg gctcagtacg gctccagcac ggacgatgtg gtaaagggct tcgtcgagca
     1081 ggcacgcgcg gaccttgcgc gactgggaag ctgacagccg gtacggttga aaannnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacatcc atctctggct tgcgcttgcc
     1261 gcgggtctgt tcttcgtgct gctctcgttg accgatttct acgaactgat cgaactgaag
     1321 acgtacgatt accgactgat tctccggaac aagattttcg gcccgccgca ccagctcccc
     1381 aatcttgcca cgattgacgt agatgactac tccgtggaca aggaagggcg ttttgaggac
     1441 tgggaccgca cctatcacgc gcggataatt gacatcgcat cggcgctggg tgcgcgggct
     1501 atcggcgttg atttcctcat gccggaaccc agcacaccca tgatccggga gaaccaggtt
     1561 gccgagagcg atgttcactc gcgggaagca gtgcttgcac ttttccgcaa cccggacgtc
     1621 gtgctgtctg acgcctgccg caagtggaac aacgtctact tcgcgcagta tctgaccgag
     1681 gcggagacgc aggattacga ccgctcgctt cgggagaatc cgccgcgcac ggaggtggaa
     1741 gagcaccgtt tccagctcgt gcaacggttc acgattccga tcacgcagga tttccagaag
     1801 gagttcgtgg tcgggtctca gctctgggcg ccggtggata cgttcctggc aacggcccga
     1861 ggcgcgggtc aggttcagcc gattccggat atggacggta ttgtgcgccg gaaccgggcg
     1921 ttctacgtgt acgatggccg gattttccct tccctttcga tcgtgatggc cgcggactac
     1981 ctcggcgttc ccctgagctc attcaagttt gagccgggac gtgtcaccct gccgaacgct
     2041 catattccgg gcgagccggc gccccgcgac attgtcattc cgcttggtgc gcgggggaca
     2101 attctcgtga actgggcggg ggactaccgg agcacctacc ggcatttccc gtatgccagt
     2161 gtcaagacct tctgggaagt tcaccagcgc gagcaactgg ccggtcttgt gaaacgcgac
     2221 cttgcgcgcg atcccgcgct ccttgatggc ctcatgggcg gccagattga cctttcggag
     2281 ttcacgctca gccgaatgat ggagctttcg cgcgttccgt cggtggacgt caaaatgggc
     2341 ctccaggatg tagccgtggc cctgaagatg agcggcgcgg tttcccgcag ggcaccggtg
     2401 accgaggcgc tgcgagatgc gttcggcaaa acgaccggca tacccgacat cgggcgttgg
     2461 cgtgccctgt acgaggaact cctgctcaac ggacgggtgg cggacctgtt cgccgggcgt
     2521 cccgacctaa cgcttgctga tgccgcgcgg gatctcaaca tgccggctgc gcggctggag
     2581 agcacgtaca cgaagattcg tacgctcgtt tctccactca ccggggagat cgcgccgggc
     2641 aattacccgc tctggttctt cgcgcagacg cgggacggtg ctcccgtcgc agtcaatgac
     2701 agcgtgaaca gcgtgctggc gagcgccatg aaggaattcc ttcggaggca cggtgtcata
     2761 agcgatgcgg tggtgacggg gatcgtgacg ccgactcccg aaaccggcgc tgatcttacc
     2821 aacctcgcgg tccgtgtggt cgcgtttgcg gcgggatacc cggagcagga ttacgtcgcc
     2881 gtgacaggaa gtatcgttca ggccgcgctg ctcgaacccc tggtggccga gggcacgtcg
     2941 ttcgacgaca tcgtgcaaca gcttttccag gtgtctccct cagatttgcc ggccgaagtg
     3001 atacagccgt accgcgatac ctacaacacg ctctggagga acaacaatct tgcagccgtc
     3061 ctgaaggcgg acacgagcct ttccgtggag gccgcggccg acacgctgac cgttctgctc
     3121 cgaaagagcg cgggaaatga ctcggtcgca gtttcggctg ttgacatcgt ggccgagcat
     3181 gcgatcctgc tgaagcatct gaaggaacaa ggggacattc cgccggaggc gcacccgctc
     3241 gtgttcaaca cggttgtggt ggacggcaga ccagtgttcg tatccgactt tgaaggggcc
     3301 gtgttgttct acggcatcac ctccaccggc gggcatgaca gaaaccccac cccctttgag
     3361 ccgcgctacc cgatggtggg aatgcatctg aacctgttca accaggtcgt gaccggcggg
     3421 ttcctgtatc aggctccgaa atggctggac tgggcggtaa ttctggcagt tgcgcttctc
     3481 atggggcttc tcgtgcccaa aatgtccccc gtgaatggcg gaatcctcgc atttgccatg
     3541 gtggtggtgt tcctgacgag cacggtggtg ctgttcgcca aggggcgaat ctggattgac
     3601 acggtggggc cgtttggcgc gatcacgttc gggtatctcg tgatcaccgt ccgcaactat
     3661 atcgtgggag agaaagagaa gaagttcatc aaaggggcct tctcggcata tcttgcgccg
     3721 gaagtggtgg aaatcatcgc caatgacccg gaagggttga agctcggggg atcaccggaa
     3781 catattacgg cgtttttcag cgacgtaaag ggattctcca cgatttcgga agcgctcagc
     3841 ccgaccgagc tggtgaatct gcttaacgag tacctgaccg aaatgtgcga tatcgcgttc
     3901 aagtatggcg cgacggtgga caaattcatc ggcgacgcga tcgtcgcgtt tttcggggcg
     3961 cccataaagt atgaagacca cgcggtccgg gcgtgcctct gcgcgtgcga gatgcagtcg
     4021 cggctggcgg tgcttcgtga gaaatggaag gcggagggca agcacgagat ctactgccgc
     4081 atcgggctta acaccggaaa cgcggttatc ggcaacatgg gcggccgtca gcggttcaac
     4141 tacacgatga tgggcgacac ggtgaacctt gccgcgcggt tggaaagcgg cgcgaagcag
     4201 ttcggcgtct acacgatgat caccgatgtt acctacaacg cggctcgcga cgtgatcgaa
     4261 gcccgtgagc ttgataggat cgtcgtgatt ggcaaaaaag agccggtgac catctacgag
     4321 gttctgtcca agaagggcga actcgacccc aaaaagaaac agatcgcgga cttctatgca
     4381 gaaggccttg cgtactaccg cgaacagaac tgggatgccg cggtggcaaa gtggcgtgcc
     4441 gccatggcgg ttgaccccga ccatccggac gcaacctccg gcatcttcat caagcgcgtc
     4501 gatgcgatac gcgcggggaa ggtggagatc ccgaaggatt ggaacggcgt ctgggaaatg
     4561 acggagaagt aaatcgcagg agcgttgcac atcaccccct ccgactcgta ctgcggcgga
     4621 gggggtgtgt ttttccggga cggccccatg ggattccctt cacccgtgaa cctcagcgtt
     4681 tcgctgctgg tggttatgtc atccacggcc tttgcttctc ctttcgtcgg ttccccccgc
     4741 gaatacgcac tcggaatagg cgcgctgggc acggccgccc tgctggaaca gtggccgccg
     4801 aatcgttccc atccgcttat cggcggagaa cagaagcctt atatcgaacg ggaacgcgtg
     4861 cccgactggg cgctggcagg agcgcatctc gtcacgatcg cggcaatccg cttttcggac
     4921 agccctgaca gggtacggta tgcccatggc tacgggatgg cagttgcgtt gacgcaggca
     4981 accacgtcgt tcacgaaggg gatcgtcgga cgaaagcggc ccaacgagga tgcggcggtt
     5041 gctctgggaa agagcagcaa atcgcggagt ttcttcagcg gacacgcgag tacgggattt
     5101 tgcctggcca cgtattcctc cgcgtacgca tggcgtgcct cgccgaagct ggaggtcaga
     5161 gcaggtgttc ccctcgcaac gtacggcgcg gcgacgtatg cggcgtggag ccgcgtggcc
     5221 gaacaccggc actatcccgc tgatgtaatt gccggggcgg ccgccggcac ggccatcggc
     5281 gcacttgtat tccgctggta cgatgggatg gaggcgggcg gccgggcggg cgcctccgcc
     5341 agcgtgtttc ctctgccgcg gggcgccgcg gtgtcggtag cattctgagg cggcaacaag
     5401 ccgcctgcgg aggaacgcct gcgttctccg ggaacctctc ttcgcttact ctttccccgt
     5461 ttgaacttca cttcagcgca acagggtcac ccttctcgtc aacacgccct cgcgcacggt
     5521 cagccgcacc agatacacgc cgctggcgac ggccctgccg ttgttgtcgc aaccgtccca
     5581 aactgcttca tggtggccag acaggaatgt ccggcccacc agatttcgca cctgtctgcc
     5641 gttgacatca tacactgcca gcgttacata cccggcgtcg ggcagaccga agcgcagcgt
     5701 ggtggacggg ttgaaagggt tgggcgtgtt ctgatcgagg taaaacgcac gcgaggacgg
     5761 cggcgcggcg acgtcggcgg gattcgtgaa tttcgccgtc actgtcgtgt ccgtggtcag
     5821 ggagagcgtc agggtgtcgc tgagggagat gatccccgcg gcggtctccc acccggcgaa
     5881 tacgtacccg ggtttgggcg ttgccacgag gcgtaccggg acatcacgga agtagacgcc
     5941 gcgccatggg tacaccagat gcgaaacccc ggggaacccg tcctcgatcg tgatgctgtt
     6001 gatccgaacc gtcccgcagt tctgtgccgg accgtctgtg ttgatggtta cggccgcggt
     6061 gtcagccgtg taggttctga agtagcccag gatgtgctgg atcatgtgcg gccggcgtgc
     6121 ggtcgcgaaa ttgcggagcg tctgaacgta tgcggtccag tagtacaggt attttggctc
     6181 gccccaccgt gcgatgtgct ccggcactcc gggtgcgatc tgcgctgcga gagaatcgat
     6241 catgccgatc atccgcggtt cagtgaacgt ggagttcagt tggtcggcaa gcgtgttgat
     6301 gaacattgtg cggaaatcgg ggttgagcag gagccaccgg aggagccagg tggattcctg
     6361 attcgggttg ccgatggtcg cccagatcag ggtgttctgg tctgcctgtg agctttcccg
     6421 gagcccgaac ccgcggtcga agtcgtacgc cagccacctc cagcgcccgt ccaactggcc
     6481 ggcgctcggg gaatagggga cgcgggcccg ccagacgcgc acgttcttgt gtggccagtc
     6541 cgtattagcg acaaagatgt tcgcggcgca gtactcggcg aagttttcca catccacgag
     6601 cgttttgaag tgctcatata cctgcgggtc tgtggcggaa tggtttttgg tgtaggagag
     6661 catatccgtg aatgccaccg catcaccggg gtcggcatca tacgggccgc cggggaggct
     6721 gtagtcgatt tcgatcagat ccacggcgga aggatcggcg ccgtgatgtg tctgaaggta
     6781 gtattcgtcg taccggtccc gaaagaactg aataccccag tagtcgccgt tgaagaacac
     6841 cactacaggc cggtacttct gcgtgtcgaa gctcaggtgg ctcacgaggc tctgcatcac
     6901 cccgtcgcgt atgagtgcgg tgtagatatc gcttgcgccg gtgcgcacga gaatgcgctt
     6961 gaagcgggtg atgtcgcttt cggggaacaa ccgcgtttca aaccagtcct tgccgtacga
     7021 tgatttcgca tagaggcgga acggcttctg gaaataatcg cgggtgtagt ttccatggat
     7081 gcgcattccc gcgccctgcg agaaggcgag tgttccgtct tcttccatga gctccacgtg
     7141 caccgggcgt tcccagtcgc ccccgctctg gtagaagttg gcgtcttcct tcaacccggt
     7201 ctgtgcgtac ttgctgcctg gcacgtagat accgatggtg tcgtcaaaaa ggttcaagga
     7261 atccgttacc agcgacagca cgggaaaggg ataacgcgcc cttcccttcg gggtgacgaa
     7321 ataggttccg gttaccgttt tgcttggcag acagcccgtt ttgaatgccc gtgcacggac
     7381 aggcacgccg cggaacacgt tgccggtcgg ggcatgccag acgttctgcg ggttggtcgt
     7441 gcggatgaga aggattcccg atgccggacc ggtgcggtct tcgattcgta acgcaccgga
     7501 atacacgggg gacgtctctg tcggctccga cccgtccacc gtgtaccgaa ttgtcgcctg
     7561 tgcgtcgctg gacgagatgg agagatcaaa cggagccggg tagaaccctc ccgagtgaga
     7621 aaacgcgggt ggctcgagaa cagcctgccc gccgccggca ttgctccggc cgggggttgc
     7681 cgcggcaaaa aacaccagat ccgcgccgcc atccggggta cggccccagg agacgtcctt
     7741 gcccatggct gcgcgcggca ggctatcctg cagcgtgcca tccggtcgcg taagaacgat
     7801 cgtttcgccg tcggagctga ttttgaaatt tgtgtggagc gcgccaaccg gatccgtcga
     7861 atctttgccg gacgcccaga caagcaggaa ctcccccggc gcaatgctga ccgcggggaa
     7921 tacccactgg aaggggttcg aaggggtatc tgtgagcccg tatccgccca gttggacggc
     7981 atcggtgctg tcgttgtaga tctcgatcca atcggcgtag gtgcctgcgg catcggtgat
     8041 ggttgcgctg ttcgaggcca tcacctcgtt gagaacaacg gttcctccgt gtgcgctggt
     8101 cgcgagaaat gcggccgata gagctgccgc ccgtaagaaa ctatgaaaca tcccatttct
     8161 cccatccccc gatgtactac ggttcttact gataaggtac ggcattcggc ccaaaaccgg
     8221 aggggtaacc gtgcattcct caggggttga aggagttgac cggctcgccc ccgggatacc
     8281 gccgccgccc accggtcggt tggtcaggcg ggtcgaaggg cgcatattct ggtggtgcac
     8341 aagggcaccc ggttgcggaa cgaaatccaa cgaaggaaca gacgcatggc caccgatcgc
     8401 gagctgtttt cgcccctccc gcgcgaagaa gtgatcaaag ctgtggagcg cagaaagccg
     8461 gcacggattc cgcttgtgat ggcgaagtgg tggggagagg gtctgggaga ccaatacggc
     8521 gaccgcctgc gggaattcga ccgcatcccg gacgacgtgg gaatgctctg gtggggaccg
     8581 ctggacgtgg gcaaaatggg gctctcgtgg caggcaaaga gtggcgccgc gcacgacagc
     8641 cattgtgtga tagacgactg gaaccatctt gacgaattca tcgaaaaaat gccggatccg
     8701 gagcgtgacg acgccttttc cggacttctg gaacatgcgg agcggatgcg gaaggacgac
     8761 cggtacatcc tcttcggctg gtgggggttg ttctttgaac gcccgtgggg tttgcgcggc
     8821 atggagaacc tcatggcgga ctactacgaa gcgccggagc acgtgcaccg tctgcacagc
     8881 gctcttgcgg acctctacat cggctacatc cggcgggccg cacgggagtt tcaaccggac
     8941 gggttctgga cgagcgacga tctgggccac cagacgcagt ccatgatgcg gcgggagcag
     9001 ttcgacgcgt tgctgaaacc gtactacgat cgcgtgggga aggcctgcaa agaagccggt
     9061 atgcactggt ggctccatag ttgcggcaac aacaccgttc tgctgccatc tctgatcgac
     9121 gcgggagttg acgtgtttca ccccgtgcag aagcacacga tggacgaaat tgccgttgcg
     9181 cgggagttcg gcgggaagat tacgttcctg gcgggaattg acgtacagca cacgctgcag
     9241 gaaaagacgc cggaaggggt gcgcgaagag gtccggttcc tcattgatac gttcgatcag
     9301 ccggatggcg ggatgtgtat cgcgtcaggg aacggcattg tcagcggcac gccttttgag
     9361 aacatcgagg catttctgga tgagtcggtg cggtacggag cagcccatcg acgcaggttc
     9421 gcctgagccg ggtcaggcgc tgttcaggga aggcgctgcc ggtttgacac gatcttcaag
     9481 gagaaatagc aaaaacagga aggaaatgac cgaaggtctt gacggcgtgt cggcgggaac
     9541 tccgtgctac gccgtcttct ttttttctgt cgacggcgtt tccagcgtct gcaccggaac
     9601 gaatcgcccg cagtcgccct tcacgtcgtc gtggcagtgt tcttccgggt ggttggcgga
     9661 cgccgcgcac agatcctcgg caatgtagtt cttgcactcg ggtttcgagc ttttcatcgc
     9721 gtttcctccg cgtgttccgg cgcacgtgtt ttccatgtac caaagatagc ggagcggatg
     9781 ccggttgtca aagaacaggc gcgccgaacc cggcgccgtc ctctcacccc acaaagggtc
     9841 ggttgacaca ggactgagtt cccgcaacat ttctggcaac ccggacacaa ccggtcgcat
     9901 cattcattcc atttcggggg aggtgtgtga tggacaggac gaaacggctc gtgattgcga
     9961 cggtgatggg gttcgtatgc gggttcgtgt gccttggtct tgcggcaagc gggccagggg
    10021 acgtttcgat cccggttgca ctgacgctca tcaccgggcg ggc
//
LOCUS       DAOSOO010000108        10023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6723_length_10023_cov_5.466794, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000108 DAOSOO010000000
VERSION     DAOSOO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6723_length_10023_cov_5.466794"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="PLT86_08700"
     CDS             complement(<1..1892)
                     /locus_tag="PLT86_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOT36730.1"
                     /translation="MPSESESGILIRPTQQALRQYYAELASLAGHGATNETTIRVAFQ
                     HLLEKTCRERKWTLLTEHHMKVNGREIVLDGALVDEYNQQRGAWEAKDAKDDLGREIA
                     AKRADGYPLKNTIFENSRRAVLYQDGREVMSTDITKPENLANLLTQFYRYSEPHIEEF
                     HRAMEEFKERVPDLARGLLEIIQKAHTDNPAFIAAFDDFFRLCRTSLNPNMSEATVDE
                     MLVQHLLTERIMSEVFDYSDFRQKNAVAAEVEKVIAALAGKSFSRADFLKSLDRFYVA
                     IENAARTVSDFTEKQHFINTVYERFFQGYSVKIADTHGIVYTPQPIVDFMCASVEQVL
                     KDEFGLSLGSPGVQILDPCTGTGSFIVNLLGRIPPTDLKRVYREQLFANEIMLLPYYI
                     ASLNIEHRFWELSGSYEPFEGLCFVDTLELAEPKQTSLFVEKNTERVTREKGAPITVI
                     IGNPPYNVGQENENDANKNRKYPVVDRRIRDTYAKDSTATNRNALSDMYVKFFRWATD
                     RLEGRDGVVCFVSNNSFVDLIAFDGMRKHLLKDFTHIWHIDLHGNVRQNPKLSGTTHN
                     VFGIQVGVGITIAVRNAGADLRVRPQGAPKVGMDAVTCADTGAHTGAPLRYHRVPEDW
                     RKEEKWA"
     gene            complement(2379..3599)
                     /locus_tag="PLT86_08705"
     CDS             complement(2379..3599)
                     /locus_tag="PLT86_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT36731.1"
                     /translation="MLKNPMTDRQRRLKWFRDARFGMFIHWGLYSMLGRHEWVMNRER
                     IPLKEYEKLADKWQPKPNCMRDWAKLAKKAGMRYMVMTTKHHEGFCLFNTKHTDYNAV
                     KRSPGRDLVAEYVDACRAEGLKVGFYYSLMDWHHPDGVKCMKNDASWVRFTEFTHGVV
                     RELCTNYGKVDIMWFDGGWPLSAEGWRSQELINMILDLQPDCIINNRTGLAGDFGTPE
                     NRIVPDKERMWEACMTFNHSWGYTPIDTDYKSAWEVLSMLRQCAAGGGNLLLNIGPKP
                     DGSLYHPCDTELERVGRWMKKYGKSIYEATDPIPQDWMITGAFTRKGDTLYYHCNRWP
                     GEELAIGGLRCKILSAKIMGGAVLKFTQKADRLVIKGLPEKAPDVLSTPIELKFEGEA
                     RHELGPGHKLLSEV"
     gene            complement(3623..4639)
                     /locus_tag="PLT86_08710"
     CDS             complement(3623..4639)
                     /locus_tag="PLT86_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36732.1"
                     /translation="MTPKEIVWRAVHRERPPRLPVSAGSLGVEDRYGVPIHSLFQEED
                     GNRRVDEWGCVWEHTDVPGMGQVKVHPLEDVSKLGAYRFPDYTDDRRYTDVQAALEQA
                     NREEKYVIAGIFLVLFERMHMLHGFENTLVDLYQDRPAMEALADGIVDTHVTLVREMA
                     RRFPGKIDGWTMTDDWGTQQSAFVSFDLWMDFFFPRYSRIFDAMHAAGCDVWVHSCGK
                     VNEIIEGYIRAGADIVNLCQPRALGIEEIGRRYRGRISFESVADIQMTLPTGDRGLIA
                     ADIEALMTHWASPEGGFYFSDYGQGAAIGVNDESIKEFEYDEFSRWSERLYGEPLPPR
                     RNTH"
     gene            complement(4668..6026)
                     /locus_tag="PLT86_08715"
     CDS             complement(4668..6026)
                     /locus_tag="PLT86_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36733.1"
                     /translation="MFTPTPPIFTPPRMYVLKEVWQNPQAARRAERLAASWPGIDVRT
                     FTYDTLPDIVVEEGWDHGAKMGTMAQVPPPIPILGLFRFDRDAVAADARRMSEAYKGT
                     GGFPFNLAAGDGAFVFFCSSTRNYPVKTLKDIKPCPEHVCRPQWRIHQGRGCPHQCAY
                     CSLGGYLITHVNTEDYIERLADLLAQNPWQKTWLYDDVMDVLTLEPELDTLAPLMRFF
                     ERTGDRYLILHTKSDRVHGLIEANAPHNTIIAWSLSGPTQSRRIERVAGTTESRVEAA
                     RQCQDAGMTVRYKFKPIVPIKNWREEAEYTVDLALRRTKPDNLSMTTLMWMDLAELTR
                     CIPEDLLDAEFLQAARNAHEEMKDSRVGPYPHAVREEIYRHYLKAIRNRHPDIPVTIS
                     TESLEMWKSLGTDLGFTPATYVCGCGAGATPNLLKLQTNPWQDARAARTWDGAPAMPE
                     GE"
     gene            complement(6220..7587)
                     /locus_tag="PLT86_08720"
     CDS             complement(6220..7587)
                     /locus_tag="PLT86_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT36734.1"
                     /translation="MTCPNILFVFSDQQRWDTLGCYGQPLNTTPNLDQMAREGVRFEN
                     AFTCQPVCGPARSCLQTGLYATQNRCPTNHCMLPLDSTTIAHHLSGAGYEVGYIGKWH
                     LSSFGPRGGPDDFRERPVPPERRGGYKDYWLASDTLEYTSHSYDGHMFDADGNRRDFP
                     EGRYRVDAETDWVIEYLESRDGKRPFFLFASYIEPHHQNDHGHYEGPHGSKERFKDFV
                     VPGDLVGTEGDWREEYPDYLGCIASLDENVGRIRAALARLGLAENTLIIYTSDHGSHF
                     RTRNSEYKRSPHDGCIRVPLIMCGPGFRGGTMPTELVSLIDLPPTILAAAGVTPPEIM
                     RGTPVQRVVAGDVADWQDDVFLQISESQCGRAIRTKKWKYSVVAPGKTGHDPSSDLYV
                     EEYLYDLEADPHERTNLVDDSSLADVRAEMAARLKRRMMDAGEEEPAILSRKEYDGRT
                     PTQAG"
     gene            complement(7859..9697)
                     /locus_tag="PLT86_08725"
     CDS             complement(7859..9697)
                     /locus_tag="PLT86_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36735.1"
                     /translation="MSLHIPPEATEGAYGRITLDAKKYRPLDTVTATIHGRAQGDHAC
                     TVRVCDPEQRPYLEARVVLSENRGSVSFAAAGKLGAHYVYLIWEGETRHSRFVNFQLE
                     AETCIESGDADFDVLYAFTRDAMRQGRREYDTPDGKLVGYISADTWHLDGIWLRDWIY
                     GLPAYRWWERDMQCGLDRFLDRQEASGMLHDGIERDGRTWRVGLESDVEYIMVLAVWQ
                     TWQATGDDEWMARALPKLERALKYIRNDPLHWDEGTRLVKRQHSCDTWDFDIDGASDK
                     GNQRHVIATCDQSGYYLAFRAMAAMYRYLGLEFEAAVWDGEADEYRERAHTLLWDGTR
                     YLHHHHIDEIDHADFDERDQLAMGNTWAMTRGMAQAGQARSIIEDYRRRHAETGDAYP
                     WWSLQPGYPDYLGYFKAPFLRQGGYANGGLMPWVGGELCRAAFLFGRERYGVDLLRAY
                     ADHLRRTGGAHVWYWPNGETGFRTTNEVPYASWGMAEWLNAAVEGFAGIHDDEALFRQ
                     VTLSPRWAAAGVGEVRVCARYAASKGYVAYRFRADDTKREIRFTCTGSGKAAYVRVLL
                     PEGREASSVRCGGQEMEFAIETEDVSRYVCFAMPLDAQREAVIACE"
     gene            complement(9836..>10023)
                     /locus_tag="PLT86_08730"
     CDS             complement(9836..>10023)
                     /locus_tag="PLT86_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36736.1"
                     /translation="KSGLRVHPVHPHERTDPAGGLSEVSPFSSHMPGTNHCKSALRRL
                     PQLSPQPLTVDFLGTTG"
BASE COUNT         1669 a         3219 c         3073 g         2062 t
ORIGIN      
        1 gcccatttct cttccttgcg ccaatcctcc ggcacacggt ggtaacgtag gggcgcacct
       61 gtgtgtgcgc ccgtgtcggc gcaggtgacg gcatccattc ctaccttcgg cgctccctgc
      121 gggcggacac gcaggtccgc ccctgcgttg cggacggcga tggtgatgcc gacgccgacc
      181 tgaatgccga acacattgtg cgtcgtgccg ctcaatttgg ggttttgccg cacattgccg
      241 tgcaaatcga tgtgccagat gtgggtgaaa tctttgagca ggtgcttccg catcccgtcg
      301 aaggcgatca gatcgacgaa gctgttattc gacacgaaac acaccacgcc gtcccgaccc
      361 tcaagccggt ccgtcgccca ccggaagaac ttcacgtaca tgtcggaaag ggcgttccgg
      421 ttggtggctg tggagtcttt cgcgtatgtg tcgcggattc ttcggtccac caccgggtat
      481 ttgcggttct tgttcgcgtc gttctcattc tcctgcccta cgttgtacgg cgggttgccg
      541 atgatgacgg tgataggggc tcctttttcg cgcgtcaccc gttcggtgtt cttttccacg
      601 aaaagacttg tctgcttcgg ctcggcgagc tccagcgtat ccacgaagca caggccctcg
      661 aacggctcat agctgccgct cagctcccag aaccggtgct cgatgttgag cgaggcgatg
      721 tagtagggca gcaacatgat ctcgttggcg aagagctgct cgcggtagac gcgcttgaga
      781 tcggtgggcg ggatgcgccc gagcaggttg acgatgaagc tgccggtacc ggtgcacggg
      841 tcgaggatct gcacgccggg gctgccgagg gagaggccga actcgtcttt cagcacctgt
      901 tccacgcttg cgcacatgaa atccacaatg ggctgcggcg tgtaaacgat gccgtgcgta
      961 tcggcgattt tgaccgaata cccctggaag aaccgttcgt acaccgtgtt gatgaagtgc
     1021 tgtttctcgg tgaagtcgct caccgtgcgc gcggcgttct cgatggcgac gtagaaccgg
     1081 tcgagggact tcaggaagtc ggcccggctg aagcttttgc ccgcaagcgc ggcgatcacc
     1141 ttctccacct cggcggccac cgcgtttttc tgccggaaat cggagtaatc aaacacctcg
     1201 ctcatgatac gttcggtgag caggtgttgc accagcattt catccacggt ggcctcgctc
     1261 atgttcgggt tgagcgaagt ccggcacagg cggaagaaat cgtcgaacgc ggcgatgaag
     1321 gcagggttat cggtgtgcgc cttctggatg atctcgagga gccctctggc gaggtcgggc
     1381 acgcgctcct tgaactcctc catggcccgg tggaattcct cgatgtgcgg ctcggagtag
     1441 cggtagaact gggtgagcag gttggcgagg ttttccggct tcgtgatgtc ggtgctcatc
     1501 acttccctgc cgtcctgata cagcactgca cgccgggagt tctcgaagat ggtgttcttc
     1561 agggggtagc cgtccgcccg tttcgccgcg atttccctgc cgaggtcgtc ctttgcgtct
     1621 ttcgcctccc acgcgccgcg ctgctggttg tattcgtcca ccagtgcgcc gtcaaggacg
     1681 atctcccgac cgttcacctt catgtggtgt tcggtcagga gcgtccactt gcgttcccgg
     1741 caggttttct ccagcaggtg ctgaaacgcc acgcggatgg tggtttcgtt ggttgcgccg
     1801 tgtccggcga ggcttgcgag ttcggcgtag tactggcgga gcgcctgctg cgtgggcctg
     1861 atcagaattc ccgattcaga ttcggacggc atggccggat tcatcctcca cggtgaatac
     1921 gggtggtagt aaggagaata tacgggggtt ggggggctcc ctcacccctc ctgcgcgatg
     1981 cgcaggcaac ccctctcccg aagggcgagg ggagaaccag cgctggtatc agttccctca
     2041 cctctgctcc ccctcacccc ccgacccctc tcccgaaggg cgaggggaga acaggtgcac
     2101 cccctcttcc accctcgggg gaagggaagt cgcgcatcgc gcggcaggga tcagggggaa
     2161 aggtccccac cgccaacgac gcgttgacga ggtacaaggc ggcgccaatc tacccgcggg
     2221 atgaggaagc aagagtggca tcagcctccc tcacctctgc tccccctcac ccccaacccc
     2281 tctcccgaag ggcgagggga gaacaggtgc accccctctt ccaccctcgg gggaagaggg
     2341 ggttgttcgc cctgcgaacg gggggtgatg agggaacgct acacctcgct cagcaacttg
     2401 tgccccggac caagttcgtg ccgcgcctcg ccttcgaatt tcagctcgat gggcgtggaa
     2461 agcacatccg gcgccttctc gggaaggccc ttgatcacca ggcgatcggc tttctgcgtg
     2521 aacttgagca ccgcgccgcc catgatcttc gccgacagga ttttgcaccg caggccgccg
     2581 atggcaagtt cttctcccgg ccaccggttg cagtggtagt acagcgtatc gcccttgcgc
     2641 gtgaacgcgc cggtgatcat ccagtcctgc gggatcggat cggtggcctc gtagatgctc
     2701 ttaccgtact tcttcatcca ccggccgacg cgctccagct cggtatcgca cgggtggtaa
     2761 aggctgccgt cgggcttcgg gccgatgttg agaagtagat tgccgccgcc cgccgcgcat
     2821 tgccgcagca tggagaggac ctcccacgcg gacttgtagt ctgtatcgat cggcgtgtag
     2881 ccccagctat ggttgaacgt catgcaggct tcccacatgc gttccttgtc cggcacgatg
     2941 cggttttccg gcgtgccgaa atcccccgcg aggccggtgc ggttattgat gatgcaatcc
     3001 ggctgcaagt cgagaatcat gttgatcagt tcctgcgagc gccacccctc ggcggaaagc
     3061 ggccacccgc cgtcgaacca catgatgtcg accttgccgt agttggtgca gagttcgcgc
     3121 accacgccgt gggtgaactc ggtaaagcgc acccaggagg cgtcgttctt catgcatttc
     3181 acgccgtccg ggtggtgcca gtccataagt gagtagtaga acccgacctt caggccttcg
     3241 gcgcggcacg cgtccacgta ctcagcgaca agatcccgcc ccggcgagcg ttttaccgcg
     3301 ttgtagtcgg tgtgtttcgt gttgaacagg cagaagccct catggtgctt ggtcgtcatc
     3361 accatgtagc gcattccggc ctttttcgcc agcttcgccc agtcgcgcat gcaattgggc
     3421 ttcggctgcc acttgtccgc gagtttctcg tattccttga gcgggatgcg ttcgcggttc
     3481 atcacccact cgtgccggcc cagcatggag tagagtcccc agtggatgaa catgccgaac
     3541 cgggcgtcgc ggaaccactt gagccttcgc tggcggtctg tcatcgggtt tttcagcatg
     3601 gcgtgaccct cctgtggatg aatcagtgtg tatttctgcg tggcgggagc ggttcgccgt
     3661 agaggcgctc cgaccaccgg ctgaactcgt cgtattcgaa ctccttgatg gattcgtcgt
     3721 tgacgcctat ggcggccccc tgcccgtaat cgctgaagta gaagccgcct tcgggggatg
     3781 cccagtgcgt catgagggct tcaatgtcgg ccgcgatcag ccctctgtcg ccggtgggca
     3841 gcgtcatctg aatatcggcg acgctttcga acgaaatgcg tccgcggtag cgtctgccga
     3901 tctcctcgat tcccagcgct ctcggctggc agaggttcac gatatccgcc cccgcccgga
     3961 tgtagccctc gatgatctcg ttcaccttgc cgcacgaatg aacccagaca tcgcacccgg
     4021 cggcgtgcat ggcgtcgaag atgcgcgagt accgggggaa gaagaaatcc atccagaggt
     4081 cgaagctgac aaacgcgctt tgctgggtgc cccagtcgtc cgtcatggtc cagccgtcga
     4141 tctttccggg gaagcgccgc gccatttcgc ggacgagcgt tacatgcgtg tccacgatgc
     4201 cgtcggccag cgcctccatg gcggggcggt cctggtaaag gtcaacaagc gtgttttcga
     4261 agccgtggag catgtgcatg cgctcaaaca gcacgagaaa gatgccggcg atgacgtact
     4321 tctcttcccg gtttgcctgc tcaagcgcgg cctggacgtc ggtgtaccgg cggtcgtcgg
     4381 tgtagtccgg gaaacggtac gcaccgagtt tcgagacgtc ctcgagcggg tggaccttca
     4441 cctgacccat ccccggcacg tcggtgtgct cccagacgca tccccattcg tctacccgcc
     4501 tgttcccgtc ttcctcctga aaaaggctgt ggatggggac gccgtaccgg tcttccaccc
     4561 ccagtgaccc cgccgaaacg ggcagccggg gcggcctctc cctgtgcacc gccctccaga
     4621 caatctcttt tggcgtcatc gacatctccg gggttcttgg gcccgtgtta ctccccctcc
     4681 ggcatggccg gtgcgccatc ccatgtgcgc gctgcccgtg catcctgcca ggggtttgtc
     4741 tggagtttga gcaggttcgg ggtcgcgccg gcgccgcagc cgcagacata cgtcgccggg
     4801 gtgaagccga ggtccgtgcc gaggctcttc cacatttcga ggctctccgt cgagatcgtc
     4861 accggaatat ccgggtgacg attccggatg gccttgaggt agtgccggta gatttcctcg
     4921 cgcaccgcat ggggatacgg accgacgcgc gaatctttca tctcctcgtg cgcgttgcgc
     4981 gccgcctgca ggaactccgc gtcgagcaga tcttccggga tgcaccgggt cagctccgct
     5041 aagtccatcc acatgagcgt cgtcatgctg aggttgtcgg gtttggtcct ccgcagggca
     5101 aggtccacgg tgtattccgc ctcttcgcgc cagtttttga tcggaacgat gggcttgaac
     5161 ttgtagcgga ctgtcatccc ggcgtcctga cactgccgtg ccgcctctac gcggctttcc
     5221 gtagtgccgg cgacccgttc gatccggcgc gactgcgtcg ggccgctcag gctccacgcg
     5281 atgatggtgt tgtgcggggc attcgcttca atgaggccgt gcacgcggtc gcttttggtg
     5341 tggaggatga ggtagcggtc accggtgcgt tcgaaaaacc gcatgagcgg cgccagcgtg
     5401 tcgagttccg gctccagcgt cagcacgtcc atcacgtcgt cgtagagcca ggtcttctgc
     5461 cacgggttct gcgcgagaag gtcggccagc cgttcgatgt agtcctctgt gttcacgtgg
     5521 gtgatgaggt atccgccaag ggagcagtac gcgcactgat gcgggcatcc gcggccttgg
     5581 tggatgcgcc actgcggccg gcagacgtgt tcgggacagg gtttgatgtc cttcagcgtc
     5641 ttgaccgggt agttccgggt gctggagcag aagaacacga acgcaccatc gcctgccgca
     5701 aggttgaagg ggaagccacc tgtgcccttg taagcttcgc tcatacgtct cgcgtccgcc
     5761 gccacggcgt cccggtcgaa acggaacaac ccgaggatgg ggatcggcgg tggaacctgc
     5821 gccatagttc ccatcttcgc gccgtgatcc cacccctcct cgacaacgat gtcgggcagt
     5881 gtgtcatacg tgaaggtgcg gacatcaatt cccggccagg aagcggccag ccgctccgcc
     5941 cgccgcgcgg cctgagggtt ctgccacacc tctttgagca cgtacatgcg cggcggggtg
     6001 aagattgggg gggtgggagt gaacaccggc gtttcccgaa cgggctaagt gcccatggtg
     6061 aaagtcagga ccctcaacgg cggaccgtca ttcccgcgaa ggcggcaatc cacatgccgt
     6121 cgctcactga ggaaccgaac tcagacagag caacctgaat gcggcaaaca ggacgtatgt
     6181 tacccatggg aatgtaccca cctcacagcc ccagtcaact caaccggcct gtgtgggggt
     6241 tcgcccgtca tactccttcc ggctgagaat tgccggttcc tcctctcccg cgtccatcat
     6301 gcgccgcttg agccgtgcgg ccatctccgc gcgcacgtcc gccagtgagg agtcatccac
     6361 cagattcgtg cgctcgtgcg ggtcggcttc gaggtcgtag agatactcct ccacatacag
     6421 gtcgctggac ggatcgtgcc cggttttccc cggcgccacg acgctgtatt tccacttctt
     6481 cgtcctgatg gcccgcccgc actggctctc gctgatctgg aggaacacgt cgtcctgcca
     6541 gtccgccaca tcgcccgcaa cgacccgttg caccggtgtt ccccgcatga tctccggcgg
     6601 cgtcaccccg gcggcggcga gaatggtggg cgggaggtcg ataaggctga caagttccgt
     6661 cggcatggtg ccgccacgga accccgggcc gcacatgatc agcggcactc gtatgcaccc
     6721 gtcgtgcggg gaacgcttgt actccgagtt ccgcgtgcgg aagtgcgagc cgtggtcgct
     6781 ggtgtagatg atgagggtgt tttcggcaag ccccagccgt gccagcgccg cgcggatccg
     6841 cccgacgttt tcgtcaaggc tcgcaatgca cccgaggtag tccgggtatt cctcgcgcca
     6901 gtcgccctcc gtgccgacaa gatctcccgg cacgacgaag tccttgaacc gctccttcga
     6961 accgtgcggc ccttcgtagt gcccgtggtc gttctggtgg tgtggctcga tgtaggacgc
     7021 gaacaggaaa aacggtcgct tcccatcgcg cgattcgagg tattcaatca cccagtccgt
     7081 ctcggcgtcc acccgatacc ggccttcggg gaaatcgcgc cggttgccgt cagcgtcgaa
     7141 catgtgcccg tcgtagctgt gggaggtgta ctccagcgtg tccgatgcga gccagtagtc
     7201 cttgtagcca cccctccgct cgggaggcac cggacgctcg cggaagtcgt ccggtccacc
     7261 acgcgggcca aacgacgaca ggtgccattt gccgatgtag ccgacctcat atcccgcgcc
     7321 ggagagatgg tgcgcgatcg tcgtggaatc gagcggcaac atgcagtggt tggtggggca
     7381 tctgttctgc gtggcgtaaa gcccggtctg caggcacgaa cgcgccggtc cgcacaccgg
     7441 ctggcaggtg aacgcgttct cgaatcgaac gccctctctt gccatctggt cgagattcgg
     7501 cgtggtgttc agcggctgcc cgtagcagcc aagggtatcc caccgctgct ggtcgctgaa
     7561 cacgaacagg atgtttggac aggtcatatc ggtccccttt gtcctatggc ttggctgttc
     7621 gtcagatgcc ctcgcttact cggcacacct catcgagcgc ctgcgaaagg aatcctcgct
     7681 gttcgtccgc ccggcggaca acgctcagaa tgacggcccg acaccagacg ctctggttcc
     7741 cagcttgttc gccatcccgt tgcctgagat gcgtcattct gagcggagcg aagaatctcc
     7801 ttcccgattt ctccttcttt tctttttcgt gtagttcgtg tgtttcgtgg ttcccaactc
     7861 actcacacgc gatcacagcc tcccgctgcg catccagcgg cattgcgaag cacacgtacc
     7921 gcgagacgtc ctcagtctcg atggcgaact ccatttcctg cccgccacac cgcactgacg
     7981 aggcttcccg tccttccggc aggagcaccc gcacgtaggc ggccttgccc gatcctgtgc
     8041 acgtgaagcg gatttccctc ttcgtgtcgt ccgcccggaa ccggtaggcg acatacccct
     8101 tggaggcggc gtagcgtgcg cacacacgga cctcgcccac accggcggct gcccagcggg
     8161 gcgacagggt cacctgccgg aacagcgcct cgtcgtcgtg tattcccgca aaaccttcca
     8221 cggcggcgtt gagccactcc gccatgcccc acgaggcgta cggcacttcg ttcgtcgtgc
     8281 ggaaaccggt ttcgccattc ggccagtacc agacgtgcgc accgcccgtg cgacgcaggt
     8341 gatcggcgta cgcccggagc aaatccacgc cgtaccgctc cctgccaaac aggaacgccg
     8401 cacggcacag ttcgccgcca acccacggca tcaagccgcc gttggcgtat cccccctgcc
     8461 gcagaaacgg ggccttgaag tagccgaggt aatcaggata acccggctga aggctccacc
     8521 aggggtacgc atcgccggtt tccgcgtgcc ggcgccggta gtcctcaatg atgctgcgcg
     8581 cctgcccggc ctgcgccatg ccgcgtgtca tcgcccatgt gttgcccatg gcaagctgat
     8641 cgcgttcgtc gaaatccgcg tggtcaatct cgtcgatgtg gtggtgatga aggtacctgg
     8701 tgccgtccca gagcagcgtg tgcgcgcgtt cgcggtactc gtccgcctcg ccgtcccaca
     8761 ctgcggcttc gaattccagc ccgaggtacc ggtacatggc ggccatagcc cggaaggcga
     8821 ggtagtatcc actctgatcg cacgtggcga tcacgtggcg ctggttgcct ttgtcggacg
     8881 cgccgtcaat gtcgaaatcc cacgtgtcgc aactgtgctg gcgcttcaca aggcgtgtgc
     8941 cttcgtccca gtggagcggg tcgttgcgga tgtacttcag cgcgcgctcc agcttcggaa
     9001 gcgcccgtgc catccattca tcgtctcccg tcgcctgcca cgtctgccat accgccagca
     9061 ccatgatgta ttccacgtct gattcaagcc ccacgcgcca ggtccgcccg tcgcgctcaa
     9121 tgccgtcgtg cagcatgcca cttgcttcct ggcggtcgag gaagcggtcg agtccgcact
     9181 gcatgtcgcg ctcccaccag cgatacgcgg gcaggccata gatccagtca cgcaaccaga
     9241 tgccatccag atgccatgtg tcggcggaga tataccccac cagtttcccg tccggcgtgt
     9301 cgtattcccg gcggccctga cgcatggcgt cccttgtgaa ggcatacagc acgtcgaagt
     9361 ccgcgtcgcc ggattcgatg cacgtctcgg cctcaagctg gaaattcacg aagcggctgt
     9421 gccgggtttc tccctcccag atgagataga cgtagtgcgc gcccagcttc cccgcggcgg
     9481 cgaacgacac actgccgcgg ttttcactca acaccacccg tgcttccaga tacggcctct
     9541 gttccggatc acacacgcgg acggtgcacg cgtggtcgcc ttgtgcgcgg ccgtgtatcg
     9601 tcgccgtaac cgtatccagc gggcggtact tcttcgcgtc cagcgtgatg cggccatacg
     9661 caccttctgt cgcttcgggg gggatatgaa ggctcatgag ttctcccaca caggcagatt
     9721 cggacacacg tcgtcgccgg gcaacggaga cgggtcaggg cacagtacgc cgaccggttc
     9781 gtttctggga tagtacgcac cgccgccctt gctcccaaag cgcctgcccg cctgctcatc
     9841 ccgttgtacc gaggaaatcc accgtgagtg gttgtggaga aagctgcggc agacgtctga
     9901 gcgcgctttt gcagtggttc gtacccggca tgtggcttga aaacgggctc acctcggaaa
     9961 ggccgcccgc agggtcggtc cgttcatggg ggtggacggg gtggacacgg agaccgctct
    10021 tct
//
LOCUS       DAOSOO010000109         9832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6882_length_9832_cov_6.734377, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000109 DAOSOO010000000
VERSION     DAOSOO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6882_length_9832_cov_6.734377"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..288)
                     /locus_tag="PLT86_08735"
     CDS             complement(1..288)
                     /locus_tag="PLT86_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOT36737.1"
                     /translation="MSDTTTHDSGMAASATNGAVRPGMIVPAIIEGIAPDGRGVASAS
                     GRQLLVRGALPGDEVDARVFRRRGGRLEATCVSVRREGYPRREPRCAHTGQ"
     gene            complement(377..862)
                     /gene="rplQ"
                     /locus_tag="PLT86_08740"
     CDS             complement(377..862)
                     /gene="rplQ"
                     /locus_tag="PLT86_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT36738.1"
                     /translation="MRHGNKGRRLGVTTPHRRSMMANLAVSLIQHGGINTTEARAKEL
                     RPFVEELVTLAKRGDLHARRLVTARLRDPGATKKLFDEWAPKFVSHPTKAGDGWNGGY
                     VRILRLGLRKGDSASISLVQFVTAESAESSASRKPAQEKKSEPAKSSSEKDNAASEET
                     S"
     gene            complement(865..1857)
                     /locus_tag="PLT86_08745"
     CDS             complement(865..1857)
                     /locus_tag="PLT86_08745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT36739.1"
                     /translation="MPDAVEVDQASTQTYAKFVLQPLERGFGTTLGSSLRRTLLSSME
                     GAAILAVRIEGVQHEFTAIPGVVEDVPELVLNLKQVRLRLHTDEKKTIHVRKKGPGEL
                     VAGDLAVDSAVEIVNPKLVLATLSEGAEIAMEVDLGKGRGFLLAEEMESEDIPIGTIP
                     IDAVFSPITKVDFEVGNARIGQKTDYDKLTLEVWTDGTTTPRSAVAFAVRLLREHLQL
                     FMGESEREETGARGVLAAAPTSVLDTRVEDMDLSMRSINCLRAAGITTVQELVLKSEN
                     DMLKYRNFGRKSLIELTEKLESLGLRFGMSPEELAEVRAQLSGGSAPEEKSEEA"
     gene            complement(2023..2652)
                     /gene="rpsD"
                     /locus_tag="PLT86_08750"
     CDS             complement(2023..2652)
                     /gene="rpsD"
                     /locus_tag="PLT86_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT36740.1"
                     /translation="MARYDGPVCRLCRREGTKLFLKGERCNSEKCSLDRRNYAPGQHG
                     QGTQRKVMEYGRQLREKQKARRTYGVLERQFRRYYEMAVRIPGVTGENLLRLLEQRLD
                     NVVYRLGFAPSRAAARQLVRHRHIEVNGRPVSIPSFLVRPGMTVSVRQGSKEMPSIHE
                     SLRQLGKSREMSWLVVDKVALSGQMVQLPSREDIQIPLEERLIVELYSK"
     gene            complement(2678..3076)
                     /gene="rpsK"
                     /locus_tag="PLT86_08755"
     CDS             complement(2678..3076)
                     /gene="rpsK"
                     /locus_tag="PLT86_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT36741.1"
                     /translation="MASPTGRARKGRKKDRKVEATGVAHVKATFNNTQVTLTDKDGNL
                     ISWSSAGKAGFKGSRKSTPFAAQSAASQAAREAMDLGLRKVEVLVKGPGVGREAAVRA
                     LQAAGLEISAIRDVTPIPHNGCRPPKRRRV"
     gene            complement(3135..3521)
                     /gene="rpsM"
                     /locus_tag="PLT86_08760"
     CDS             complement(3135..3521)
                     /gene="rpsM"
                     /locus_tag="PLT86_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT36742.1"
                     /translation="MARIAGVDLPREKRVDIALTYIYGIGPASSAKILQETGVDPSRR
                     MNQLTEDEIARLRSVIENQFRVEGALRSEIEMNIRRLQEIGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRAVAGARKAGKKTK"
     gene            complement(3570..3686)
                     /gene="rpmJ"
                     /locus_tag="PLT86_08765"
     CDS             complement(3570..3686)
                     /gene="rpmJ"
                     /locus_tag="PLT86_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT36743.1"
                     /translation="MKVRSSVKKLCANCKIIRRRGVVRVICKSSPTHKQRQG"
     gene            complement(3711..3929)
                     /gene="infA"
                     /locus_tag="PLT86_08770"
     CDS             complement(3711..3929)
                     /gene="infA"
                     /locus_tag="PLT86_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT36744.1"
                     /translation="MPKEDSIEVEGTVTKALPNASFKVRLENGHEVSAHISGKMRVNF
                     ITIKPGDKVKLELSPYDLTRGRITYRHR"
     gene            complement(3945..4595)
                     /locus_tag="PLT86_08775"
     CDS             complement(3945..4595)
                     /locus_tag="PLT86_08775"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT36745.1"
                     /translation="MRLILLGGPGSGKGTQGAVLAQKFAIPTISTGDLFRKNIADNTP
                     LGQKVKQYTDQGALVPDAVVLDMVFDRLVQADCSAGYLLDGFPRTIPQAEALTKWLSE
                     KNAAIDHVVLIDVDDEVIVDRLSSRRTCESCGAIVSVKFSPPKKEGVCDLCGGKLVQR
                     ADDNPGTVRDRLQVYHKNTEPLIAYYRQQGLLRAVDGSASPELVTKAILAAVGAKS"
     gene            complement(4625..5965)
                     /gene="secY"
                     /locus_tag="PLT86_08780"
     CDS             complement(4625..5965)
                     /gene="secY"
                     /locus_tag="PLT86_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT36746.1"
                     /translation="MLERFRNIFNIPELRNRILFTFGVLIVYRIGGAIPTPGVNSAAL
                     AQAFSARARDLFAVYDLFAGGAFKQATIFALGIMPYISASIIFQLLGSVWAPIQQLQK
                     EGQEGQKKINQYTRYLTVALAAVQSLGVSQFLIQNGAALDPGIGFRLMVMLTMTTGTV
                     FVMWLGEQISERGIGNGISLIIFIGIVARFPHAVIGQLRVMLDPDSGGVIATMALFAL
                     MFVITAAVVLITQGTRRIPVRYASQRVVGGRVYGGQSTHLPLKVNAAGVIPIIFAQSI
                     VLVPTTVLQFAGTATPESETLQMVFGWFQPTSLIYWTFYSLLIIFFSYFYTAIIFNPV
                     DVADNLKRNNGFIPGVRPGKKTAEYIDRVLNRITLPGSIYLALIAALPFLIISIFQSF
                     RVSVVPDFHDFFGGTGLLIVVGVALDTLDKVESHLLVRHYDGFMKGSRLRSRRR"
     gene            complement(5958..6410)
                     /gene="rplO"
                     /locus_tag="PLT86_08785"
     CDS             complement(5958..6410)
                     /gene="rplO"
                     /locus_tag="PLT86_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT36747.1"
                     /translation="MKLGDLVPAEGARKNRKRVGRGNGSGHGTFSGRGCKGTKARSGT
                     GRMRVGFEGGQMPIQRALPARGFRNPAKKSFQLVNVGDIAARTLGDRVDAAALHAAGL
                     IRSAVEPVKVLGDGELNRAVTVVATMFSRTAREKIAAAGGTAEESTGA"
     gene            complement(6424..6603)
                     /gene="rpmD"
                     /locus_tag="PLT86_08790"
     CDS             complement(6424..6603)
                     /gene="rpmD"
                     /locus_tag="PLT86_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018663943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT36748.1"
                     /translation="MKKITVTLYRSPIGRVAYQKRVVKGLGLHKVGQTVEHEDKPDIR
                     GMVAKVPHLVRINEA"
     gene            complement(6600..7199)
                     /gene="rpsE"
                     /locus_tag="PLT86_08795"
     CDS             complement(6600..7199)
                     /gene="rpsE"
                     /locus_tag="PLT86_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT36749.1"
                     /translation="MARDGGYSRGGNVEESEFVETVVKVKPVSKVTKGGRDRRFNALV
                     VVGDKKGRVGVGMGKAREVSEAIRKGTQIAQRSIQRVSVGVTIPHEVWGRCGAGLVFL
                     RPATPGTGLIAGSAARAVLEAAGVENVLSKSVGSANALNVARATLDALAKLRTFTQVR
                     SERRLIPPETVAAETVDIAPAENAENEAPVETPNEGAEA"
     gene            complement(7221..7568)
                     /gene="rplR"
                     /locus_tag="PLT86_08800"
     CDS             complement(7221..7568)
                     /gene="rplR"
                     /locus_tag="PLT86_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT36750.1"
                     /translation="MSERRIRRVKRWRLAKPIRGTAERPRLVVSRGLRTIQGHFVNDE
                     IGKVICGASTASKKLAIAGRGCEAAKSLGKELARVAREHGITRTVFDRNGYLYHGRVK
                     ALAEGAREGGLEF"
     gene            complement(7610..8179)
                     /locus_tag="PLT86_08805"
     CDS             complement(7610..8179)
                     /locus_tag="PLT86_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT36751.1"
                     /translation="MSRIGRRAIPLAKNVAVAISGDTLTAKGPLGEVSVKIPAGVNVK
                     IDSDQVTVEPEIGASPGAWGLARTIIANAVEGVSKEFERRLFVVGVGYRAEADGSKLT
                     LSVGHSHPVTIQADRGVTFAVEPAITLPGKKDNYPAIPVVVKGVNKEAVGDYAAKVRA
                     VKPPEPYLWKGIRYQNERMRTDKAGKTAK"
     gene            complement(8190..8588)
                     /gene="rpsH"
                     /locus_tag="PLT86_08810"
     CDS             complement(8190..8588)
                     /gene="rpsH"
                     /locus_tag="PLT86_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT36752.1"
                     /translation="MSMTDPIADFLTRIRNACKAGHRKVDIPASTLKREVARILLEQG
                     YVEDVYVVDDGKQGMLRIYLRYLRDGKPVIRGLARRSKPGLRRYNGAQTMDRVLNGLG
                     IAIVSTSKGVMTDAECRRIGVGGEVLCHVW"
     gene            complement(8624..8809)
                     /locus_tag="PLT86_08815"
     CDS             complement(8624..8809)
                     /locus_tag="PLT86_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOT36753.1"
                     /translation="MAKKSWMIKSARKPKFKVRAYNRCHRCGRSRGFLRKYGVCRICF
                     RELALRGDIPGVVKASW"
     gene            complement(8838..9386)
                     /gene="rplE"
                     /locus_tag="PLT86_08820"
     CDS             complement(8838..9386)
                     /gene="rplE"
                     /locus_tag="PLT86_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT36754.1"
                     /translation="MPPRLRERYKAEIVPQLTKRFSYKNPMQVPRVLKVVVNMGVGEA
                     IQNPKAVDSAVADLQQIAGQKPSVRRARKSVSNFKLRAGVPIGCAVTLRGVRMWEFLD
                     RLLTFAVPRIRDFRGIPRRGFDGRGNFTFGVKEQLIFPEIDYDTVDQIRGMDISIVTT
                     ARTDEEAFELLSGLGMPFARQA"
     gene            complement(9586..>9832)
                     /gene="rplX"
                     /locus_tag="PLT86_08825"
     CDS             complement(9586..>9832)
                     /gene="rplX"
                     /locus_tag="PLT86_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT36755.1"
                     /translation="RAKGKVIVEGVNIIRRHTKPSRENPQGGIVEKEAPISVSKVLLV
                     SPRANRGVRVRHQRLEDGTRVRICTKTGDMIPPPSKS"
BASE COUNT         1932 a         3347 c         2490 g         2063 t
ORIGIN      
        1 tcactgcccg gtgtgtgcgc accgtggttc ccgccgcggg tatccttccc ggcggaccga
       61 cacgcacgtc gcctccagac gtcctccgcg cctgcggaac acccgtgcat ccacctcatc
      121 gcccggcaaa gccccgcgaa caagcaactg ccgccccgat gcggacgcca cgccacggcc
      181 atcgggcgca atcccctcga taatcgcagg aacaatcatt ccggggcgca ctgcgccgtt
      241 cgtcgcggaa gccgccatgc cgctatcgtg tgtcgtagta tcgctcacag cctgtccgca
      301 tcaaaagaaa cgggacgcat cagcgtggtg ccgacgacgt cccgcgttct caatacccga
      361 tgtttgatgt gcggcgctag gacgtttcct cgctcgccgc attgtccttt tcagagctgc
      421 tcttcgcagg ctctgatttc ttctcctgtg ccggtttccg gctcgccgaa gactcagcag
      481 attccgcagt gacgaactgc accagcgaga tggacgcaga gtcacccttc cggaggccaa
      541 gcctcagaat gcggacatac ccgccgttcc atccgtcacc cgctttcgtg gggtgagaga
      601 cgaacttcgg agcccactcg tcgaaaagct tcttcgtcgc tcccgggtcg cgcagacgcg
      661 ccgtaaccag gcgccgcgca tggagatcac cccgtttggc gagcgttacg agctcttcca
      721 cgaaaggacg cagttccttc gcacgtgcct ctgtggtatt gatgcccccg tgctgaatga
      781 gggatactgc cagattcgcc atcatggatc ggcgatgcgg cgtggttacg cccaggcggc
      841 gacccttgtt cccatgtctc atgattacgc ttcctcgctc ttctcttcag gcgccgaacc
      901 gccagaaagt tgggcgcgca cctcggcaag ctcttcagga gacatgccaa atcgaagccc
      961 caggctctcg agcttctcgg taagttcgat caacgacttc cgcccgaaat tgcggtattt
     1021 caacatatcg ttttctgatt tcaggacgag ttcctgaacc gtggtgatgc cggcggcgcg
     1081 caggcagttg atagaccgca tggaaagatc catgtcctcc acgcgggtat caagaacgct
     1141 ggtgggcgct gcggccagca caccccgtgc tcctgtttcc tcacgctccg attcacccat
     1201 gaaaagctgg aggtgttccc tcaaaagccg gacggcaaac gcaaccgcgc tccgcggggt
     1261 agtcgtgccg tccgtccaga cttccagcgt caacttgtcg taatcggttt tctgcccgat
     1321 acgcgcattt cctacctcga aatccacctt ggtaatcggc gagaacaccg catcaatcgg
     1381 tattgtcccg atcggaatgt cttccgattc catttcctcc gccagaagga accctctgcc
     1441 cttcccgagg tccacttcca tggcgatttc tgcgccttcc gacagcgtgg ccagaaccag
     1501 cttgggattc acgatctcaa ctgcactgtc caccgcaagg tcgccggcaa ccagttcccc
     1561 ggggcccttt tttcgcacat ggatggtctt cttctcatcc gtgtgcaggc gaagacgtac
     1621 ctgcttcaga ttcagaacga gttccgggac atcctcgacc acacccggaa tcgccgtaaa
     1681 ttcatgttgg acaccctcaa tgcgcaccgc cagaatcgcg gccccttcca tcgaggaaag
     1741 caacgtccgg cgaagactcg agccgagcgt ggtgccgaag ccgcgctcca gaggttggag
     1801 cacaaacttc gcatacgtct gcgtagaggc ctgatcgacc tccaccgcat cgggcatgac
     1861 gaatgaaggc gtttctgctg tgtcagtttt catcatggtg cctacaaccc cctcctcaga
     1921 aacgaacgac cacccgcgta ttcccgcaga acacccggta tctgccgctc gtggaacatt
     1981 tcagagcctg cgcgggcatt tcccacttca ggcacgaggg gcttacttcg agtacaactc
     2041 gacaatgagg cgctcttcca acggaatctg tatgtcctcg cgggaaggaa gctgaaccat
     2101 ctgtcccgaa agcgccacct tatccaccac cagccacgac atctcgcgcg atttcccgag
     2161 ttgcctcaac gactcgtgaa tgctgggcat ctctttgctc ccctgccgca cggaaaccgt
     2221 cattcccgga cgcacgagga acgagggaat cgaaaccggc cgcccgttca cctcgatatg
     2281 ccggtggcgc accaactgac gcgccgccgc gcgggacggg gcaaacccaa gccggtacac
     2341 aacgttgtcc agccgctgtt caagcagacg cagcaggttt tcacccgtaa cgccgggtat
     2401 tcgaacggcc atctcgtagt accggcggaa ctgacgctcg agaacgccgt acgtccgacg
     2461 cgctttctgc ttctctcgaa gctgccggcc gtattccatc accttgcgct gcgtcccctg
     2521 gccatgctgg cccggagcat aattccgccg gtcgagggag catttctccg aattacaccg
     2581 ctcgcccttc aggaacagct tcgttccctc gcggcggcaa agcctgcaga ccggaccatc
     2641 atatcgtgcc attcacgtct cctgaattct caacaaccta cacgcgccga cgtttcggcg
     2701 ggcggcaacc attgtgcgga atcggggtaa catcacgaat agcgctgatc tccagaccag
     2761 ccgcctgaag cgcgcgtacc gccgcctcac gtcccacgcc gggtcccttg acaagaactt
     2821 ccactttccg caatcccaga tccatcgctt cgcgggcggc ctgtgatgcc gccgattgcg
     2881 ctgcaaatgg cgtgctcttc cgcgatccct tgaaaccggc tttgccagcc gaagaccagg
     2941 aaatcagatt gccatccttg tcggtcagtg ttacctgcgt gttgttgaac gtcgccttca
     3001 cgtgcgctac gccagtcgct tcgactttgc ggtccttttt ccgtcccttc cgggcacgac
     3061 cagtcggaga tgccattcag taatcctctc aaaaccttcc cgatcaacgc cgctcagacg
     3121 gcggcggacg ccagttactt cgtcttcttc ccggccttgc gagctccggc caccgcccgt
     3181 cgcggcccct tgcgggtacg ggcatttgtg tgcgtgcgct ggcctctcac cggcaacccg
     3241 cgccggtgcc gaagacctcg atagcacccg atctcctgca acctgcggat gttcatctcg
     3301 atctcactcc gcagggcgcc ctcaactcgg aactggttct cgatcaccga gcgaaggcgg
     3361 gcgatctcgt cttcggtcaa ctgattcatc ctgcggctcg gatccacgcc cgtttcctga
     3421 aggatctttg ccgatgaggc cggacctatc ccgtaaatgt aggtaagggc gatatccacc
     3481 cgtttctcac gcggcaggtc aactccagct atacgcgcca ttgagatact ccctttgcac
     3541 tcacgttaaa tgatgcacga caactctgat caaccctgac gctgtttgtg cgtcggactc
     3601 gatttgcaga tcacccgcac aacaccccgg cgccggatga tcttgcagtt ggcgcaaagc
     3661 tttttgaccg aagaacgcac tttcattcga actcactcca taaaccacgt ctagcgatgc
     3721 cggtacgtga tgcgcccgcg cgtcagatcg tacggcgaaa gctcgagctt caccttgtcg
     3781 cccggtttga tggtgatgaa attcaccctc atcttgccgg atatgtgtgc cgagacctcg
     3841 tgcccgtttt ccaaacgcac cttgaacgaa gcgtttggca gtgccttcgt tacggttcct
     3901 tcgacctcaa ttgagtcttc ttttggcaca gacaccactc tcagctagga tttcgcacca
     3961 acggcggcaa gaatcgcctt cgtcaccagt tccggtgacg ccgaaccatc taccgcacgc
     4021 aacaacccct gctgacggta gtacgcgatt agcggctcgg tgttcttgtg gtacacctga
     4081 agtcgatcgc gaaccgtccc ggggttgtca tccgcgcgct ggacaagttt cccgccgcac
     4141 agatcacaca cgccttcctt cttaggaggc gagaacttca cgctgacgat cgctccgcac
     4201 gactcacacg tccgccgcga cgagagccga tccactatca cctcgtcgtc cacgtcgatc
     4261 agtaccacgt ggtcaatcgc cgcgtttttc tcagaaagcc acttcgtgag cgcttcggcc
     4321 tgcgggatcg tacgcggaaa cccgtcaagc agataccccg ccgagcaatc cgcctgaaca
     4381 agccgatcga acaccatgtc cagcacaacc gcgtccggaa caagcgcgcc ctgatccgtg
     4441 tactgcttca ccttctgccc gagcggcgtg ttgtccgcga tgttcttccg gaaaagatca
     4501 ccggtcgaaa tggtcgggat cgcgaacttc tgcgccagta ccgcaccctg agtccctttc
     4561 ccggaacccg ggccaccgag taggatcaat cgcatgagct tcccccttcc tgtcttcggg
     4621 aacatcagcg ccgccggctg cggagcctgc tgcccttcat gaagccgtca taatgacgga
     4681 cgagaagatg cgattccacc ttgtccagcg tatcgagcgc cacacccacc acgatcaaca
     4741 gccctgttcc accgaagaaa tcgtggaaat ccggcaccac cgacactcgg aaactctgga
     4801 atatggagat gatcagaaac ggaagcgccg caatcagggc cagatagatc gaaccaggca
     4861 acgtgatcct gttcagaacg cggtcgatgt attcggccgt ctttttgccg ggacggaccc
     4921 ccggaatgaa gccgttgttc cgcttcaaat tgtctgcaac atccaccgga ttgaaaatga
     4981 tcgccgtgta gaagtaagaa aagaagataa tcagcagcga gtaaaacgtc cagtaaatca
     5041 gcgacgtagg ctgaaaccag ccgaaaacca tctggagcgt ttcgctttcc ggtgttgcag
     5101 ttccggcaaa ctggagaacc gtcgtgggaa caagcacgat tgactgcgcg aaaatgatcg
     5161 ggatgacacc cgccgcgttc accttcaacg gcagatgagt gctctgaccg ccgtacaccc
     5221 tgccgccaac aaccctctgg ctcgcatacc gcaccggaat ccgccgcgtc ccctgcgtga
     5281 tcagcactac tgctgcggtt atcacgaaca tcagggcgaa aagagccata gtagcgatca
     5341 ccccgccgct gtccgggtcc agcatcacac gcaactgacc gatcaccgcg tgggggaagc
     5401 gcgccactat accgatgaaa atgatcaaac tgataccatt tccgatgccg cgctcgctga
     5461 tctgctcgcc cagccacatg acgaacaccg tccccgtggt catcgtcagc atgaccatca
     5521 gccggaaccc gatacccgga tcaagcgcgg ccccgttctg aatcaggaac tggctcaccc
     5581 cgaggctctg caccgcagca agcgccaccg tcagataacg ggtgtactgg ttgatcttct
     5641 tctgcccctc ctgcccctcc ttctggagct gctgaatcgg tgcccacacc gaacccagca
     5701 actggaagat gattgaggcg gagatgtagg gcatgatacc cagagcgaaa atcgtcgcct
     5761 gtttgaacgc gccgccggcg aaaaggtcgt acaccgcaaa aagatcccgt gcgcgagcgc
     5821 tgaacgcctg cgcaagtgcg gccgaattca cgccgggcgt cggaatagca ccaccgattc
     5881 ggtacacaat caacacgccg aacgtgaaca gtatcctgtt acgcagttcg gggatgttga
     5941 agatgtttcg gaagcgttca agcacctgtc gattcctcag cagtgccgcc cgcggcggca
     6001 atcttctctc gcgccgtgcg gctgaacatc gtggccacca cggtaaccgc cctgttcaat
     6061 tcgccatcgc ccagaacctt caccggctcc actgccgagc gaatcaaacc cgctgcatga
     6121 agcgccgcgg catccacgcg gtccccgagt gtccgcgcgg caatatcgcc cacgttcaca
     6181 agctgaaacg atttttttgc agggttgcgg aaaccccggg ccggcaatgc cctctgaatc
     6241 ggcatctgac caccctcaaa cccgacacgc atacggcccg tgcccgagcg cgctttcgtg
     6301 cccttgcagc cgcgcccgct gaaagttccg tgcccgctcc cgtttccccg gccaacacgc
     6361 ttacggttct tccgcgcgcc ttctgcgggg acaaggtcgc ccaatttcac ctgttcggcc
     6421 atgctacgcc tcatttatgc gaacgagatg cggcaccttc gccaccatcc ctcggatatc
     6481 cggtttgtcc tcgtgctcca cggtctgccc gactttgtgc agacccagcc ccttcaccac
     6541 gcgcttctgg tacgccacgc gtccaatcgg gctccggtaa agagtcaccg tgatcttctt
     6601 cacgcttccg ctccttcgtt cggcgtctct actggcgcct cattctcagc gttctccgca
     6661 ggcgcgatat ccactgtctc ggcagcaacg gtctccggcg gtataagccg acgttcactc
     6721 cgcacctgcg tgaatgtccg cagcttcgca agtgcgtcca gcgtcgcacg ggctacattc
     6781 aaagcatttg ccgagccaac cgacttgctc agaacattct caactcccgc cgcttccagc
     6841 acggcacggg cagcgctgcc agcgataagg ccggttcccg gtgttgccgg acgaaggaac
     6901 accagacccg cgccgcaccg tccccacacc tcgtgcggaa tcgtcacgcc aaccgaaacc
     6961 cgttgaatgg atcgttgcgc aatctgagtt cccttgcgga tcgcttcgga gacctcgcgc
     7021 gctttgccca tgccaacgcc aacccggccc tttttgtcgc caacaaccac cagtgcgttg
     7081 aaacgccggt cgcgtccgcc tttggtaacc ttcgaaacgg gctttacctt gacaacggtt
     7141 tcgacgaact ccgattcctc tacgttgccg cctctgctgt acccaccgtc tctcgccatg
     7201 cctgttcctt tttgaattcg ctagaactcg agtccgcctt ctcgcgcgcc ttctgccaac
     7261 gctttgacac gtccgtgata caggtacccg ttgcggtcaa acaccgtgcg cgtaatccca
     7321 tgttcacgtg ccacgcgcgc cagttctttc ccgaggcttt ttgcagcctc gcaaccgcga
     7381 ccggcgatcg cgagcttctt tgaggcggtg gacgccccgc agatcacctt cccgatttcg
     7441 tcattgacga aatggccctg gatcgtccgc agcccacgac tgacgacaag gcgcggcctt
     7501 tcggcggtgc cgcgaatcgg cttcgcaaga cgccagcgct ttacccgacg gattcgtctc
     7561 tcgctcatga tggtaaattc cccaacggcc ccgaacaggc cccagtatat tacttcgccg
     7621 tcttcccggc cttgtcagtc ctcatacgct cgttctggta ccgaatcccc ttccagagat
     7681 acggctccgg cggtttcacc gcccggactt tcgccgcgta atcgcccact gcttctttgt
     7741 tgacgccctt cacaacaacc ggaatcgccg ggtagttatc ctttttgccc ggcaatgtaa
     7801 tcgcgggttc cacggcaaac gtcaccccgc ggtccgcctg aatcgtgacg ggatgtgaat
     7861 gccccacgct cagcgtcagc ttcgaaccat cagcctcagc gcggtaaccg accccgacca
     7921 cgaacaaacg ccgttcaaat tccttcgaaa caccttccac cgcgttcgca atgatcgttc
     7981 gggcaaggcc ccatgcaccc gggcttgcac cgatttccgg ttcaaccgtc acctggtcgc
     8041 tgtcgatttt cacattcacg cccgcaggta tcttcacaga cacctcgccc agagggcctt
     8101 tggcggtcaa tgtatccccg gaaatcgcaa ccgcgacatt cttcgcgagc ggaatagctc
     8161 ttcgtcctat gcgagacaca atccttctcc taccatacgt ggcaaagaac ttcgccacca
     8221 actccgatgc gacggcattc agcgtctgtc atcacacctt tcgacgtgct cacaatcgcg
     8281 attcccagac cgttcagaac acgatccatg gtctgcgcac cgttgtaacg gcgcaaacca
     8341 ggtttcgaac gccgagccaa cccgcggatg accggtttgc catctctcag gtaccgcagg
     8401 taaatgcgca gcatgccctg tttcccgtca tcaactacgt agacatcctc gacgtagccc
     8461 tgttcgagca gaatgcgcgc tacctctcgc ttcagagtcg acgccgggat atctaccttc
     8521 cggtgtcccg ctttgcacgc attcctgatt cgcgtcaaga agtccgcgat cggatcggtc
     8581 attgacatag ttctgttcct ctaattctga accgcacgta cggctaccaa ctggccttga
     8641 ccacgccggg gatatccccg cggagcgcga gctcgcggaa gcagatccgg cacacgccgt
     8701 atttccgcag aaatccgcgc gatcttccgc aacggtggca acggttatat gcccggacct
     8761 tgaatttcgg tttacgcgcc gatttgatca tccagctctt tttcgccacg agaaccctcg
     8821 ctttccgcgt tcgcccgtca ggcctggcgc gcgaacggca tgcccaaacc actcagcagt
     8881 tcgaacgctt cttcgtcagt gcgcgcggta gtgacaatcg aaatatccat gccccgaatc
     8941 tggtcaaccg tgtcgtaatc tatttccggg aagatcagct gctctttcac gccgaacgtg
     9001 aaattgcccc ggccatcgaa cccacgccgc ggaatacccc tgaaatccct gatacgggga
     9061 acggcaaacg tcaaaagccg gtccaggaat tcccacatgc gcacgccccg aagcgtaact
     9121 gcacacccga tcgggacacc cgcgcgcagt ttgaaattcg acaccgactt ccgcgcacgc
     9181 cgcacgctcg gtttctgacc ggcgatctgc tggaggtccg caacggcgct gtccacggct
     9241 ttcgggttct gaatagcctc gcccacaccc atgttgacga ctactttcag cacccgcgga
     9301 acctgcatcg ggttcttgta ggagaaccgc ttggtaagct gcggcacgat ctccgccttg
     9361 taccgttccc gcaatcgcgg cggcaccgcc tgagccttgg gttcggcggc cttcggttcc
     9421 tgcgacttcg cacccgcggc ttttttccct ttgggagccg cttccgcggc aacctgctct
     9481 ttgggcgcct tcttcttcgt cgcgcccttt gcgtcggctc ccgcttcggc tttggccgct
     9541 tttttctttg tttcctcttt tgccatcagt ttccgctccc caccttcatg acttggacgg
     9601 cggaggtatc atgtcgccgg tctttgtgca aatacgcacc cttgtgccgt cttccaggcg
     9661 ctgatggcga actcgcacgc cgcgattcgc tcgcgggctg acaagcaata ccttcgacac
     9721 cgaaatcggg gcctcttttt ccacgatgcc accctgcggg ttttcccgcg aaggcttcgt
     9781 gtgccgacga atgatgttca caccctcgac aatcactttt cccttggctc ga
//
LOCUS       DAOSOO010000110         9832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6883_length_9832_cov_6.612151, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000110 DAOSOO010000000
VERSION     DAOSOO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_9832_cov_6.612151"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLT86_08830"
     CDS             complement(<1..302)
                     /locus_tag="PLT86_08830"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOT36756.1"
                     /translation="MSVCGTRRTGDQQGVHLIRLALFLCILAARTAAAPFGPGELLVF
                     DLKWQFVRGGVATMSIHPVPGDTSLWQIRTRARSVGVLDVFYTVRDTLSSTIRRSTL"
     gene            complement(299..775)
                     /locus_tag="PLT86_08835"
     CDS             complement(299..775)
                     /locus_tag="PLT86_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOT36757.1"
                     /translation="MERVLFICTGNSCRSPMAAGIFNRLAKQRKIRDIVAESAGVFAM
                     EGLRASASAITVAHRHGINLTRHRTRPYTPEIGEQASLILTMTAEQWYCVTESTPREK
                     TFLLTNFATVDNPGIDIVDPYGGSLETYEEVFAELEREILRAFDAIVEWVEAHPTG"
     gene            complement(798..2804)
                     /gene="mutL"
                     /locus_tag="PLT86_08840"
     CDS             complement(798..2804)
                     /gene="mutL"
                     /locus_tag="PLT86_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT36758.1"
                     /translation="MASPTYQTSDSEPPPRRIRVLPSQLANLIAAGEVVERPSAVLKE
                     LLENALDAGARRVSVELVAGGRRLVQVSDDGHGMNPDDAVLCFERHATSKIAAAGDLN
                     AIHTYGFRGEALPSIAAVSRTTLQTCADDSGLGTEVIIEGGQLRAVREVGRTRGTTVT
                     VRDLFFNTPARRKFLRTDATELRHILREMTGLALTKPDLGFTLSHGGRVLLECNPVST
                     WQERVRTVFGREMLLRSIPVDKSDGTGRLYGLLGRPGDSGGGFTEQFLLVNGRSVQSR
                     VFRKAVLDGFEATTESGRQPFFVLFLELDPATVDVNVHPQKREVRFRDDRAVYDLIFS
                     AVRSAFSDTASRERGASPEPLVVPAPAEEEGPPHNADDHVSGNEAHPTKPAVSGIPSF
                     WNAPPDRPYVPATAKGADRFHRLSDGEATYLPGTGSARATFSALPDIKPSPKARAEDA
                     KAAQVALDLVSKGPGSFSASEGLGAIAASELEKTVWQFQRKYIFVNTRDGLWIIDQHV
                     AHERILYEEAMAGFAGDVPASQQLLFPTVIELTPEQDAVFDEVEEIFRSLGFGIRRLS
                     DRKIEADAYPGALHNWEDGNVIRRMIDEMRELGFKRTGLKEQIAIAYSCHAAVKSGEH
                     LPPATMRWLIERLFATSMPYVCPHGRPIIVKIETAELDQRFGRV"
     gene            complement(2810..3187)
                     /locus_tag="PLT86_08845"
     CDS             complement(2810..3187)
                     /locus_tag="PLT86_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36759.1"
                     /translation="MPGSVVVVDRDDEMRDLFHAVLTRAGHSVRTYSRLEELSAAPEA
                     PPDVLMVDATCAPNGDLSSLVNQISAQYSSSEVVYLVNTSESALKRQLERGRIRHFVR
                     PFQIEDLRELVNTLLERRSPARR"
     gene            complement(3228..3383)
                     /locus_tag="PLT86_08850"
     CDS             complement(3228..3383)
                     /locus_tag="PLT86_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36760.1"
                     /translation="MVGEKLLAAGVITQSQLERALMEAKKNGERVGDTVVRLGFATRD
                     QVEAALK"
     gene            complement(3473..6142)
                     /gene="mutS"
                     /locus_tag="PLT86_08855"
     CDS             complement(3473..6142)
                     /gene="mutS"
                     /locus_tag="PLT86_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT36761.1"
                     /translation="MTTSETRLTPMLEQYMAVKRKHQDKLILFRMGDFYETFFDDAVE
                     VSKILGITLTSRNHGKEGDVPLAGIPHHALGSYSDKLTRAGRSFVIVEQLEDPRKAKG
                     IVKRGVTEVVTPGTTLSETLLEEKRNNFLIGISVNGGVAGIAEADLSTGEFRVTEVSA
                     NELFAEVRRIGPAEVLAARSWITEHRQAFSRQVPGPVLTPMEDWAFGIAVARERLLEH
                     FKVTSLKGFGAEELTAGTAAAGAVLAYLQENQLTSLEHIRRLNVIQRAHHLILDETTQ
                     RNLELFASLSASNPRATLIGAIDQTLTPMGGRLLRQWMMRPLKDIPQINARLDGVGWF
                     VEKSMERGQVRDMLARVRDIERIMAKVCCGRAAPRELAALAASLEAAEPLSATLGDAE
                     ADLVSHAAGEMGDTSEVSALIRHTLEDDPPATLSDGGVIRRGVSADLDELREIGHGGK
                     EWLLKLQATERERTGIPSLKVAFNRAFGYYIEISNAHREKAPPDYIRKQTLVNAERYI
                     TPELKEWESRVIGAEERAISLEYELFTRLRDSVAARADAVLQQARAIALVDVLAALAE
                     TAARNNYHRPAVALDDVISIENGRHPVVEMLLPKGSFVPNDTYLANPTDQIMIITGPN
                     MAGKSTYLRQTGHIVILAHMGSWVPADKAHIGVVDRLFTRVGAHDNLAGGESTFLVEM
                     HETANILNNATPRSLLLFDEVGRGTSTFDGLSLAWAIVEYLHGTPHKAAKTMFATHYH
                     ELVELEDILPRVKNYNVTVREWQDEVIFLRKIVRGGCDHSYGIHVAQMAGLPEKVIQR
                     AREVLASLEEKDDSLHRELGAPGRSEAEGGSAGGDADAEQIGLFAPKPPPEHPIVEEI
                     RKLDLMNMTPLEVMRAVDTWQRRLDGERDRRSG"
     gene            6381..7559
                     /locus_tag="PLT86_08860"
     CDS             6381..7559
                     /locus_tag="PLT86_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT36762.1"
                     /translation="MKKRMIADRVYWMGAIDWDRRLFDSLIPLPDGTSYNAYLVEGSE
                     KTALLDAVDPAMADELMRQLDGVARVDYLISHHAEQDHSGTLPLLAKRFPEAKILASP
                     KARGMLLDLLDLPETAITTVTDGETLSLGDKTLKFIHTPWVHWPETMVTYLEEDKILF
                     SCDLFGSHLATGDLFAKDQAQVYEAAKRYYAEIMMPFRTIIQKNLEKIGGLDAQVIAP
                     SHGPVYDKPEWIVDAYRQWVSGAPGNVVVIPYVSMHASVERMVKHLAASLADRGVRVE
                     LFNLAVTDVGKLAMALVDAGTIVVGTSTVLNGPHPLALYAAYLANALKPKARFISVVG
                     SYGWGGKTVDMLAETLAGLKAEVLEPVMVKGAPSADDLAAVDALAEAIAKKHAENGFA
                     "
     gene            7584..9644
                     /locus_tag="PLT86_08865"
     CDS             7584..9644
                     /locus_tag="PLT86_08865"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOT36763.1"
                     /translation="MRISRACAVYIPASILFFAASPEAESIPRDANGAPVIPAVFASA
                     PPHLDGILDDPVWTEAVRLDDFYVVDYDRPPSEPTTVYLACDSANLYVAARMGDRFPG
                     QIRMAETKRDGQYTNDDHFAIHFDVQHLHNTSYLFIVTPRGTQATTIPDGSPSKVEWQ
                     GDWTAAARVDSAGWSVEMVIPMVIFGRPAGQHTIGVAAHRWLPRLQEEMTWPNMGRVR
                     DDTKTGNWEGIVWPAERRRPALMPYIVAERVGGDLSAYAGADMKYTTGSGLTILATAY
                     PDYRNIESGVLGLDFSYNEQARADNRPFVTEGAAYYPESWIFYSQRIGEMYGGGKLFG
                     QLGRSRVGLLTAYDRNRVVHTAGKWTWQKSDAWNFDQHMVWRHGPADAPHPAGTPMAS
                     DHLMYVSIARFTRQVGDREEHYSTQGGFTKTASDTANGYDFEFHWNRHNGNGTFGYAL
                     GGRILSSGFIPVEGLLNPADAGQRDITASLEWEKRRERRWFTEVGYWAQLKYSERLNG
                     DLYHKDATLEGWADVTPDVAVALNGKAVDRPPYVDKTLYAWMGWNRRSFGHEGGIEAT
                     AGKVKGTDYRFVSAYQNTRIGGQTSLSLSAQGVRHVYPVGHRDRPLGGTDDRYQVIGT
                     AQFDITPEHAVSGRLIESTQGLNGYATYQQVLRRGYDLFIIIGDPSADTWTRRVALKA
                     VVVL"
BASE COUNT         1799 a         3102 c         3070 g         1861 t
ORIGIN      
        1 agcgttgatc gccggatcgt tgaactcagg gtgtcgcgga cggtgtagaa cacatccagc
       61 acccccacag aacgtgcgcg ggtgcggatc tgccacaggc tcgtgtcgcc cggaacgggg
      121 tggatgctca tggtggccac cccgcccctg acgaattgcc atttcaggtc gaataccagg
      181 agttcgccgg ggccgaacgg agccgccgcc gtgcgggcgg cgaggatgca gaggaacaac
      241 gcgagcctga tgaggtggac tccttgttgg tcaccggtcc gacgcgttcc gcatacgctc
      301 atcccgtcgg gtgggcctcc acccattcta cgatggcgtc gaacgcgcgg aggatctcgc
      361 gctccagttc cgcgaacact tcttcgtacg tctccagtga cccgccgtag gggtctacga
      421 tgtcaatacc ggggttgtcg acagtggcga aattggtgag gagaaacgtc ttctcgcgcg
      481 gcgtggattc tgtcacacag taccactgtt cggccgtcat ggtaaggatc agggatgcct
      541 gttcaccgat ttccggagtg tacggacggg tacggtgccg ggtgaggttg atcccgtgcc
      601 ggtgagccac cgtaatcgcc gaagcggacg ctcgaaggcc ctccatcgca aacaccccgg
      661 cggactcggc cacaatgtcg cgaattttcc tctgtttcgc gagacggttg aaaatcccgg
      721 ccgccatggg gctccggcag gagttacccg tgcaaatgaa cagcacccgt tccatgcgtt
      781 tcctgttgct cccgtgccta cacccgtccg aaccgctgat ccagttcggc cgtctcgatt
      841 ttcacgatga tcggccgccc gtgcgggcag acgtaaggca tcgaggttgc aaacaaccgt
      901 tcgatgagcc agcgcatcgt tgcgggcggg agatgctctc ccgatttcac cgcggcatga
      961 cacgaatacg cgattgcgat ctgttctttc aaccccgtgc gtttgaagcc gagttccctc
     1021 atctcgtcga tcatgcgccg gatgacattg ccgtcttccc agttgtggag cgctccgggg
     1081 taggcgtctg cttcgatttt ccggtccgaa aggcggcgaa tgccgaagcc cagagacctg
     1141 aatatctcct ccacctcatc gaacacggcg tcctgttcgg gagtgagttc aatcaccgtg
     1201 gggaaaagca actgctgcga cgccggtaca tctccggcaa accccgccat tgcttcttcg
     1261 tacaggatcc gctcgtgcgc cacgtgctga tcaatgatcc agaggccgtc ccgcgtgttg
     1321 acgaagatgt attttcgttg gaactgccag accgtttttt cgagttctga agccgcaatc
     1381 gctcccagtc cttccgaggc gctgaacgat ccggggcctt tgcttacgag gtccagggct
     1441 acctgggccg ccttcgcatc ctccgcgcgt gctttcgggc tcggcttgat gtcgggaagc
     1501 gcggagaacg tggcccgtgc ggaaccggtg ccgggcaggt aggtcgcctc gccatccgac
     1561 aggcgatgaa accggtcggc accttttgcc gtcgcgggaa catacgggcg gtccggtggt
     1621 gcgttccaga aactcgggat gccgctcacg gcgggtttcg taggatgtgc ttcgttgccg
     1681 gaaacgtggt cgtccgcatt atgaggcggg ccttcttcct ccgcaggagc aggcaccacg
     1741 agcggttcgg gcgacgcgcc gcgttcacgg gacgcggtat ccgagaaggc gctgcgcacc
     1801 gccgagaaga tgaggtcgta caccgcgcgg tcgtcccgaa accgtacttc gcgcttctgc
     1861 gggtgcacgt tgacatccac cgtggcaggg tccagttcca gaaacagcac gaaaaatggc
     1921 tgtctgccgc tttctgtggt tgcttcgaaa ccgtcgagca ccgctttgcg aaacacgcgc
     1981 gactgaaccg aacggccgtt cacgagaagg aactgctcgg tgaacccgcc tcccgaatca
     2041 cccgggcgcc ccagcagacc gtacaatcgg ccggtgccgt ccgatttgtc caccgggata
     2101 ctccgcagca gcatctcgcg gccaaacacc gtccgcacac gctcctgcca ggtggaaaca
     2161 gggttgcact ccagcagcac gcgcccgccg tgcgaaaggg tgaacccgag atcgggtttc
     2221 gtcagcgcga gaccggtcat ttctcgcaga atatggcgta gttcggtagc gtccgtgcga
     2281 agaaacttgc gccgcgcggg cgtattgaag aaaaggtccc gcacggtgac ggtggtgccc
     2341 cgcgtgcggc cgacttccct caccgcgcgc aactggcccc cttcgataat cacttcggta
     2401 ccgaggccgc tgtcgtcagc gcatgtctgg agcgtggtac ggcttactgc ggcaatgctg
     2461 ggaagcgcct ctccgcggaa cccgtaggtg tggatcgcat tcaggtcccc ggccgcggcg
     2521 atcttcgacg tcgcatggcg ctcgaagcac agtacggcgt cgtcggggtt catgccgtgg
     2581 ccgtcgtcgc tcacctgaac cagccgacgc ccgccggcca ccagctccac acttacccgc
     2641 cgggcgcctg cgtccagcgc gttctcgagc agttccttga gcactgcgga cggacgctcc
     2701 accacctcgc cggcggcaat cagattggca agttgactcg gcaggacgcg gatgcgtcgt
     2761 ggaggaggtt cggaatcaga cgtctgatac gtcggcgacg ccatattcct cacctccgcg
     2821 cgggggatct gcgttcaagc agcgtgttga caagctccct gaggtcctcg atctgaaacg
     2881 ggcggacgaa atgacgtatc cttccccgtt cgagttgacg tttgagggca ctctctgaag
     2941 tattgacaag gtaaacgact tcactggagg agtattgcgc agaaatctgg ttcacgagcg
     3001 acgacaggtc gccgttcggg gcgcatgtgg cgtccaccat gagcacgtcg ggcggcgcct
     3061 ccggcgcggc ggaaagctcc tccagacggg aatacgtgcg aaccgaatgg cctgcccggg
     3121 tcaacaccgc gtggaaaagg tcgcgcattt cgtcatccct gtccaccacc accactgaac
     3181 cgggcatcga tgtgtctccg tcggaaatga gcgccgacgc cggtcgatta tttgagggca
     3241 gcttccacct gatcgcgcgt ggcaaatccg aggcggacta ccgtgtcgcc cacccgttcc
     3301 ccgtttttct tggcctccat gagcgcccgc tccagttgcg actgcgtaat cacgccggca
     3361 gccagcagct tctctcctac catcgtctct cccctccacc cagtggtacg cgtggccctg
     3421 caatccagac tccgcaaaag tgaacgaatg tagctcattt tggccgggca agtcaacccg
     3481 aacggcgatc tcgctccccg tcaagccggc gctgccacgt gtccaccgcc cgcatgacct
     3541 caagcggcgt catgttcatc aggtcgagct tacggatctc ctccacgatg gggtgctcag
     3601 gcggcggttt gggggcgaac agcccgatct gctccgcgtc ggcatccccg cccgctgagc
     3661 cgccctcggc ttccgatcgg ccgggcgcac ccagctcccg gtggagggag tcgtctttct
     3721 cctcaaggga tgcgagcacc tcccgcgcgc gctgaatgac cttctccggc aatcccgcca
     3781 tctgagcgac gtgaatgccg tagctgtggt cgcacccgcc ccgcacgatt ttgcgcagga
     3841 agatcacctc atcctgccat tcgcgcaccg tcacgttgta attcttcacg cggggcagga
     3901 tgtcctcgag ttccaccagt tcgtgatagt gcgtggcgaa catcgttttc gcggctttgt
     3961 gcggagtgcc gtgaaggtac tcgacgatcg cccatgccag agacagcccg tcgaacgtgc
     4021 ttgtaccccg gcccacctcg tcgaaaagaa gaaggctgcg cggggtcgcg ttgttgagaa
     4081 tgttcgcggt ctcgtgcatt tccacaagaa acgtgctttc cccgccggca aggttatcgt
     4141 gggcgccaac ccgggtgaac agccggtcca ccacgccgat gtgcgcctta tcggcgggaa
     4201 cccacgagcc catgtgcgcc agaatgacaa tatgccccgt ctgccgcagg taggtggact
     4261 tccccgccat gttgggcccg gtgataatca tgatctggtc ggtaggattg gcaaggtagg
     4321 tgtcgttggg tacaaacgac cccttcggca gcagcatttc caccaccggg tgccggccgt
     4381 tttcgatgga gattacgtcg tccagcgcca ctgcaggccg atggtagttg ttccgcgccg
     4441 ccgtttcggc gagggctgcc agcacatcca ccagcgcgat ggccctcgcc tgctggagca
     4501 cggcgtccgc gcgggctgca acggaatccc tgaggcgggt gaacagctcg tattccagac
     4561 tgatcgcgcg ctcctccgca ccaataacgc gggactccca ctccttgagc tccggcgtga
     4621 tgtaccgctc cgcattcacg agcgtctgct tgcggatgta gtccggcggg gccttctcgc
     4681 ggtgagcgtt gctgatttcg atgtagtacc cgaacgccct gttgaaggca accttcagcg
     4741 acgggatgcc ggttcgttct cgttccgtgg cctgcagttt gagcagccac tccttccccc
     4801 cgtgtccgat ctcgcgcaat tcatccaggt cggcgctcac ccctcgccgt atcacgcccc
     4861 cgtccgaaag cgttgccggg ggatcgtcct ccagcgtgtg ccgtatgagc gcgctgacct
     4921 cagaggtatc gcccatctcg ccggcggcat ggcttacgag gtctgcttcc gcgtccccca
     4981 acgtggcgga aagcggctcc gccgcctcca gactggctgc cagcgcggca agctcgcgcg
     5041 gtgcggcgcg cccgcagcat accttcgcca tgattcgttc gatatcgcgc accctggcaa
     5101 gcatgtcccg cacctgaccg cgctccatgc tcttctccac gaaccatcct acgccgtcca
     5161 gccgggcgtt gatctgcgga atgtccttca gtggccgcat catccactgg cgaagcagcc
     5221 gcccgcccat cggggtgagc gtctggtcta tggcgccgat cagcgtcgcc ctcgggttgg
     5281 aggcggagag cgacgcaaaa agctccaggt tccgctgggt ggtctcgtcc agaatcagat
     5341 ggtgcgcgcg ctggatgaca ttcagccggc ggatgtgttc aaggctggtg agctggtttt
     5401 cctgcaggta tgcaagcacc gcccccgccg ccgccgtacc ggctgtcagc tcctccgcgc
     5461 cgaagccctt gagcgacgtg accttgaaat gctccagcag ccgctcccgc gcaaccgcga
     5521 tgccgaatgc ccagtcctcc atcggcgtca gaaccggacc gggtacctgc cgcgaaaatg
     5581 cctgccggtg ttccgtaatc cacgagcgcg ccgccagaac ctccgccggc ccgatacggc
     5641 gcacttccgc gaacaactcg ttcgcggaca cctccgtgac gcggaactcg cccgtggaaa
     5701 gatcggcctc cgcgattccg gcgaccccgc cgttcaccga aataccgatc aggaagttgt
     5761 tccgcttttc ctccagcagc gtctcggaga gcgtcgtgcc gggggtgacg acctccgtca
     5821 ccccgcgctt gacgataccc ttcgcttttc gcgggtcctc gagctgctcg acgatgacga
     5881 aactgcgccc ggcgcgggtg agtttgtcgc tgtagcttcc cagcgcgtgg tgggggattc
     5941 cggccagcgg aacatccccc tccttgccgt ggttgcgcga cgtgagggta atcccgagaa
     6001 tcttcgagac ttcgaccgcg tcgtcaaaga acgtttcgta gaaatcgccc atgcggaaca
     6061 ggataagctt atcctggtgc ttccgcttca ccgccatgta ctgctcaagc atcggtgtca
     6121 ggcgggtttc ggacgttgtc atgtgcggtc gggtattccg tgaaggaacc gtgggagtga
     6181 acaggtacac actttggaaa gtatcgctag ggaagtggcg cagacgcaat gtcgagaagc
     6241 agggggcagg gctgtccgcc cgcgcgcccg ccaccggggt gtagctccac ggttcggtgg
     6301 ttgcgtacgc gcggcggggg gtcggaaatt tagagcggga agtgagtatt gctgttctcg
     6361 aataacagga gaccgagtac gtgaaaaaaa ggatgattgc cgatcgcgtg tactggatgg
     6421 gtgccattga ctgggaccgg cgtctgttcg attcgctgat accgctgccg gacgggacgt
     6481 cgtacaacgc gtacctcgtg gaaggcagtg aaaagacggc cctgctggat gcggttgacc
     6541 ccgccatggc cgacgaactg atgcggcagc tcgatggcgt tgcacgggtg gattacctca
     6601 tcagccacca cgcggagcag gatcattcgg gcaccctgcc ccttctggca aagcggttcc
     6661 ccgaggcgaa aattctcgcg tcgccgaaag ccaggggtat gctgctggat cttctggacc
     6721 ttccggaaac cgccatcacg accgtcaccg atggggagac tctctccctc ggggacaaga
     6781 cgctcaagtt catccacacg ccgtgggtgc actggccgga aacgatggtg acgtacctcg
     6841 aggaggacaa aatccttttc agttgcgacc tgtttggctc gcatctggca accggcgatc
     6901 ttttcgcaaa agaccaggcg caggtatacg aggccgcgaa gcggtactat gcggaaatca
     6961 tgatgccgtt ccgcacgatc atccagaaga acctcgaaaa gatcgggggg ctggatgcgc
     7021 aggtcatcgc cccaagccac gggccggtgt acgacaagcc cgaatggatc gttgacgcct
     7081 accggcaatg ggtttccggc gctcccggca acgtggtggt gatcccgtac gtatccatgc
     7141 acgccagtgt tgagcgcatg gtgaaacatc ttgccgcatc actggcggat cgtggcgtgc
     7201 gcgtggaact tttcaacctc gcggtgacgg acgtcggcaa acttgccatg gcgctcgtgg
     7261 atgccggaac aatcgtggta ggcacctcca ccgtgctgaa cggcccgcac ccgctggccc
     7321 tgtatgccgc ctatcttgcg aacgccctca agccgaaggc gcgattcatc tccgtggtgg
     7381 gttcgtacgg atggggcggc aaaaccgtgg acatgctggc ggagacgctg gcgggtttga
     7441 aggccgaggt cctggagccg gtgatggtga aaggcgcacc atcggcggac gatcttgcag
     7501 cagtggacgc cctggcagaa gcgattgcga agaaacacgc ggagaacggg ttcgcgtaga
     7561 ttctccgcgt tcacaggggt tttatgcgca tcagcagggc gtgtgcggta tacatcccgg
     7621 cgtctatcct tttctttgcg gctagcccgg aggcggagtc catcccgcgg gatgccaacg
     7681 gcgccccggt tatcccggca gttttcgcct cagcgccacc ccaccttgac ggcatcctcg
     7741 acgaccccgt ctggacggaa gccgtccgcc tcgatgattt ctacgtggtg gattacgacc
     7801 gtccccccag cgaacccacc accgtgtacc tcgcatgcga ctccgccaat ctctatgtgg
     7861 ccgcccggat gggcgacagg ttccccgggc agatccgcat ggcggagacc aaacgggacg
     7921 gccagtacac caacgacgac catttcgcca ttcacttcga tgttcagcac ctccacaaca
     7981 ccagttacct gttcatcgtc acgccgcgcg gcactcaagc gacaactatc cccgacggca
     8041 gccccagcaa ggtggaatgg cagggcgact ggactgcggc ggcgagggta gacagcgccg
     8101 ggtggtcagt ggaaatggtg atcccgatgg tgattttcgg ccgaccggcc ggccagcaca
     8161 ccatcggggt agcagcccac cggtggctgc cgcgtctgca ggaggaaatg acctggccga
     8221 acatgggtcg cgtccgcgat gacacgaaaa cgggcaactg ggaagggatc gtgtggcccg
     8281 ccgaacgccg gaggcccgcg ctcatgccgt acatcgtggc ggagcgcgtc ggtggggacc
     8341 tgagcgcgta cgccggcgcg gacatgaagt acaccaccgg ctccggtctg accattctcg
     8401 ccacggcgta ccctgattac cgcaacatcg aatctggcgt actgggtctc gatttctcct
     8461 acaacgagca ggcgcgcgcc gacaatcgcc cgtttgtcac cgagggagcg gcctattacc
     8521 cggagagctg gatattttac agccagcgca tcggggagat gtacggcggc gggaaactgt
     8581 tcggacagct tggcaggagc cgcgtcggcc tcctgacggc ctatgaccgg aaccgtgtgg
     8641 tacatactgc cggaaaatgg acctggcaga aatcggatgc ctggaatttc gatcagcaca
     8701 tggtctggcg gcacggcccc gcggatgccc ctcatcccgc cggcacaccc atggcgagcg
     8761 atcatctcat gtacgtatcc atcgcccggt tcacgcggca ggtgggagat cgggaggagc
     8821 actacagcac gcagggggga ttcacgaaaa ccgccagcga taccgcgaac gggtatgact
     8881 tcgagttcca ctggaaccgc cacaacggca atggaacctt cgggtatgcg cttggcgggc
     8941 gaattctgag cagcgggttc atcccggtgg aggggcttct gaacccggcg gatgcgggtc
     9001 agcgggatat cactgcgtct ctggaatggg aaaaaaggcg tgagcgacgg tggttcaccg
     9061 aggtgggata ctgggcgcag ctcaagtatt cggaacggct caacggtgat ctctaccaca
     9121 aggacgcgac gctggaaggc tgggctgacg ttacgccgga cgtcgcggtg gcgttgaacg
     9181 gaaaggcggt tgatcgcccc ccgtacgtgg acaagacgct gtacgcgtgg atgggctgga
     9241 atcggcgttc attcggccat gagggcggca tcgaagcgac cgccggcaag gtgaagggca
     9301 ccgattaccg gttcgtttcc gcctaccaga acacgcgaat cggcggccag acctccctca
     9361 gcctgagcgc gcagggcgtg cgccacgtat acccggtggg acacagggat cggccgctgg
     9421 gcggcacgga cgaccggtac caggtgatcg gcacggccca gtttgacatt actccggaac
     9481 acgcggtatc ggggaggctc atcgagagca cgcagggact gaacgggtat gcgacgtacc
     9541 agcaggtgct tcgccggggc tacgacctgt tcatcatcat cggcgatccc agcgctgata
     9601 cgtggacgcg ccgcgtggca ctcaaggcgg tggtggtgct gtgagtgcgc aaactcgggc
     9661 tcatttgtga gtgcggcgcc cgccgcactc accgcctgtg acgaaggtac tgggcgttct
     9721 ggattcccgc tttcgcggga atgacggggt gttcccctag gctattccct cgcctgcagc
     9781 tctctgaaaa tgagcacggg cagcggccgt cgggctcctc gatacagctc gc
//
LOCUS       DAOSOO010000111         9811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_6901_length_9811_cov_5.200185, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000111 DAOSOO010000000
VERSION     DAOSOO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_9811_cov_5.200185"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLT86_08870"
     CDS             complement(<1..272)
                     /locus_tag="PLT86_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT36764.1"
                     /translation="MPSYDTGNSALERARVAAEWLRERGVVEPAGLIVLGSGWSGLAE
                     MAEPIGAWDYSGIPGLPETTTPGHPGRLMYAVWHGVACVVLVGRWHV"
     gene            complement(283..1356)
                     /locus_tag="PLT86_08875"
     CDS             complement(283..1356)
                     /locus_tag="PLT86_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOT36765.1"
                     /translation="MFGTTMDAIAKRKQFRAKRCSSYDRSGRNADWVLVKARSTHVMA
                     DIKGAGVISHLWFTIAGHGRDNDYLRKMVFRAYWDGETQPSIETPVGDFFCVGHGAVV
                     SFQNAAFNMSANEDGYGKNAAMNCWLPMPFRTNARLEITNDAEVDIGMYYYVDYQEHD
                     RLDDDLLYFHAQWRRNNPCDGWKGPGSVWGSHEHGVRHNGPDGVNLSGEKNYVILEAE
                     GQGHYVGCNISIHNLYKGWWGEGDDMIFVDGEVWPPSLHGTGSEDYLCHAWGMQKNAF
                     LYNGLSYSEVEKEFNDRGKVTVYRLHLQDPVPFTRSLKVTIEHGHANDRSDDYSSVAY
                     WYQTEPHKPYPEMPGVVARLPNP"
     gene            complement(1426..2661)
                     /locus_tag="PLT86_08880"
     CDS             complement(1426..2661)
                     /locus_tag="PLT86_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36766.1"
                     /translation="MGLTTSDKQYLRGLAQRVAEIAADPYYAERREMWYDFNDLNPRR
                     PMVLIFPEGAWSELLPDSTMKLEDPFWRSVEWWLRYTIYRGERLRDDNVIDPVFELSP
                     SRGAAGWGLETRMKPTTTERGARAYDPVMKTPEDFAKMRRPHLVVNHEVNKRNYSRLY
                     DAFGDILHIRWKRSPGINTSLVNFVCYLRGLEQVMVDMVDRPDWLHEVFSFLLENTRV
                     LLDELEASGQLELMDGADYAGSGGTCYTRQLPASDYAGKARLKDTWGFAESQEYALVS
                     PRMYYEFGIKYQAPLLERFGLNCYGCCESLTDKLDLVIQHIPKLRRISISPWTDVRVA
                     AEKLQNKYVFSWKPNPVCVSNPYDPQTIRASIRETLEITRGCVVEMILKDTHTCNNVP
                     GRMEEWVRIALEEAERAAG"
     gene            2944..3669
                     /locus_tag="PLT86_08885"
     CDS             2944..3669
                     /locus_tag="PLT86_08885"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOT36767.1"
                     /translation="MRTPSRGEETPLPIRRETPTHWIYLHGFGSDPMSPKAQFFNQQF
                     RAAGLPIVVPDLNVPSFEQLTITACLAEVERVISGFPSEAKVGIVGSSLGGLIALFAA
                     DRCKNVSYVMLLAPALGLFRVNFVGLGKPGVRAWEKNGFLEVFHSPSNRNRRLSADFV
                     ADARQYDERNLRLSIPVTIVHGQRDDIVDPHLSITYARAHHNVKLHLVDDDHSLLASI
                     TQIWEWLRMDTRPRPNRDNASAH"
     gene            complement(3673..4401)
                     /gene="rph"
                     /locus_tag="PLT86_08890"
     CDS             complement(3673..4401)
                     /gene="rph"
                     /locus_tag="PLT86_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT36768.1"
                     /translation="MPNRPDGRQPDEMRPVKITRGVSPYAEGSVLIEIGGTHVMCTAT
                     VEESVPAFLRGQGNGWVTAEYGMLPRSSSIRIPRESATGRIKGRTHEIQRLIGRSLRA
                     VIDLACLGERQIILDCDVLQADGGTRTASITGSWIAMADAVRWMSEKNMIRKDPLLDQ
                     VAAVSVGISDGLPVLDLCYEEDSRADVDLNLVMTGTGCFVELQGTAESVPFTDDQLAQ
                     MITLGKKGIAELLAIQKRVCEDSL"
     gene            complement(4469..6172)
                     /locus_tag="PLT86_08895"
     CDS             complement(4469..6172)
                     /locus_tag="PLT86_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOT36769.1"
                     /translation="MSRKLFCFSCWVGNRLAISARVYLRIVPCLALWCAACARVGSPG
                     GGPEDTIPPRVVRVAPEDSATRVSLDMNPRIEFSERMNKASADQAIFITPYPVRYPRF
                     EWSRGDRVLTIRFEGGLEPQKTYVITIGTGVTDLHGVRLASAQAFAFSTGDSIHRGRV
                     QGVVYDGRFVPTSGLTIGLFPLQDNPSPDPAREYAAYVTQSGNAGRFSIGYLPDGTYR
                     LFAWRDANSDGLIDAEEPVGLASTGVVVKDGAHVRIPSVCMGLLDRTGPRLERIRALD
                     ARHVEMLFGEPVDSLRMEVVEPASGATPIECSVLKTPRASNRVVVYCSGLKPGAAYGA
                     HVRAWDRAGNPAEWAADTTSFVASGQAVTMRLRAESVEDNRAISPDSPAGFSVWTNDL
                     AVPATGFGRAFQDDGTIVEGAWHQRHANHFMFAANTLMGSRERNWVVPLADLRDDRGN
                     PCEDSVRVTLYPAREDSMGEVSGVVSDSIPGRNLRVLLRKTEEGPIFPAVVSDSLSWH
                     ANGLPAGDYEVFAWYDEDGNGRWSPGNIIPFAPAERWAVFGTVAIRPRWTTAGVVVHF
                     E"
     gene            complement(6117..7046)
                     /gene="argF"
                     /locus_tag="PLT86_08900"
     CDS             complement(6117..7046)
                     /gene="argF"
                     /locus_tag="PLT86_08900"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT36770.1"
                     /translation="MKPVRHLVSMSDFSEKEINGLIDLARKLRVEWRSGIPRHTLASK
                     TLAMVFEKPSLRTRVTFESGMTQLGGHAIYLAPGDIQIGKREPVADVARNLSRWVQGI
                     MARTYSHDTVAGLAEHAAVPVINALSDLEHPCQALALAMAIRDRRELRGTHVVFVGDG
                     NNVAHSIMLLCAITGMRFTISCPAGYEPSEVIREKCRELRGTCAIVDEPETAVRDADV
                     IYTDVWASMGQEAETETRRAAFSRYQVNGALLRSAPEHAVVSHCLPAHRGEEITSEVL
                     DSARCIAFDEAENRLHVQKAVLLFLLGGESVGD"
     gene            complement(7056..8417)
                     /gene="hslU"
                     /locus_tag="PLT86_08905"
     CDS             complement(7056..8417)
                     /gene="hslU"
                     /locus_tag="PLT86_08905"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOT36771.1"
                     /translation="MPVPAVAGAFTPAQVVAYLDRFIVGQQKAKRAVAIALRNRWRRQ
                     RVEGNLRNEITPNNILMIGPTGVGKTEIARRLAALAGAPFVKVEASKFTEVGYVGRDV
                     ESMVRDLAEAAIGLVRSERLESVREPARRAAQDRLLDVLLPPGSGDEQPTAETDDGAR
                     ERVRRRLRAKLEAGEFDERIVELDLPQNRMPLLEVFTPQGGMEEMGMNLGSVFGNMFP
                     GRTKRRKVKVREALRILEQEEAGKLIDSEDVTNEAIRRAEELGIIFIDELDKVAGREG
                     KGGPDVSREGVQRDLLPVVEGTTVSTKHGAVRTDHVLFIAAGAFHVAKPGDLIPELQG
                     RFPIRVELDSLGADDFVRILTEPQNALVKQYTALLETEGVRVEFVPEAIRKIAEVANE
                     VNSRAENIGARRLHTVMTVLLDDILFAVPDEQIREVVITPERVETRLRSILQDDNLSR
                     YVL"
     gene            8622..9446
                     /locus_tag="PLT86_08910"
     CDS             8622..9446
                     /locus_tag="PLT86_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOT36772.1"
                     /translation="MAIRLVAIDIDRTLLNDRRELSPANESALRDLVERGISVALCSG
                     RDLPSTQAISAPLGLPLWLVVQNGSLVIDPSGNAAYLCSMEKPTALAVLDILERHGLA
                     PVVYEVYPRAHCLWWQTGARAAPGMIEFRTQHGAQVSHVEDIRSVLTPEVSHLEVFDD
                     ADRVVRAGQDFATLDEVVAITNMSSSVVGSALMGLYPRGTAKEEALARVCDVLRISRT
                     EVLAIGDNLNDVGMVRWAGIGVMMANGPEEARLEADWVAPSNNENGVAVAVERFTK"
     gene            complement(9436..>9811)
                     /locus_tag="PLT86_08915"
     CDS             complement(9436..>9811)
                     /locus_tag="PLT86_08915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT36773.1"
                     /translation="LAHRVEAGADMFLMPSKYEPCGLTQMYSMRYGTVPIVHATGGLA
                     DTVTNYDGRTKRGNGFSFSRYSVDALLTAVRRAMKFFNDRERWESIMRAGMRQDFSWS
                     AAAEQYRVLYDELLNRADHTTS"
BASE COUNT         1989 a         3099 c         2635 g         2088 t
ORIGIN      
        1 acatgccagc ggccaaccag caccacgcag gcaacgccat gccacaccgc gtacatcagc
       61 cgccccgggt gccccggagt tgtggtctcc ggaagccccg gtattcccga gtaatcccac
      121 gccccgatcg gttcggccat ctccgcgagg ccagaccacc cgctgcccag aacgatcaaa
      181 cccgcaggtt cgacaacgcc cctttcacgc aaccactcag ccgcaactcg cgctctctcg
      241 agcgccgagt ttccagtgtc gtaggagggc atccccgtct tttcagggat ttggcagcct
      301 ggcgacaaca cctggcatct cggggtaagg cttgtgcggt tcggtctgat accagtacgc
      361 aacggaagag taatcgtccg accggtcatt cgcgtgcccg tgttcgatcg tcaccttcag
      421 cgacctggtg aaaggaacag ggtcctgaag gtgcagacgg tacacggtaa ctttgccccg
      481 atcgttgaac tccttctcca cttcgctgta cgaaaggccg ttgtacagga acgcgttctt
      541 ctgcatgccc cacgcgtgac acaggtaatc ctcacttccg gtaccgtgca gactgggtgg
      601 ccacacttcc ccatcaacaa aaatcatgtc atcaccctcg ccccaccagc ccttgtaaag
      661 attgtggatc gagatgttgc agcccacata atgcccctgc ccctctgctt cgagaatgac
      721 gtagttcttc tctccggaaa ggttgactcc gtccggcccg ttgtgtctca cgccgtgctc
      781 atggctgccc cagactgatc ccggcccttt ccacccgtca cacggattgt tgcgccgcca
      841 ctgcgcatga aaatagagca gatcgtcgtc aagccgatca tgctcttgat agtcaacgta
      901 gtagtacatg ccgatgtcaa cttccgcgtc atttgtgatt tcgagtcgcg cattcgtccg
      961 gaaaggcatg ggaagccagc agttcatcgc cgcgtttttg ccgtaaccgt cctcatttgc
     1021 agacatgttg aatgccgcgt tctgaaacga aactactgct ccgtgcccga cacagaagaa
     1081 atcgccaacc ggggtttcga tcgagggttg ggtttcgccg tcccagtacg cacggaacac
     1141 catttttctc agataatcgt tatcccgccc gtgtcccgca attgtgaacc agagatgact
     1201 gattaccccg gctcccttga tatccgccat gacgtgcgtg cttcgagcct tgaccagcac
     1261 ccagtcagca ttccggcccg agcggtcata gctcgagcag cgcttcgctc gaaactgctt
     1321 tcttttcgca atcgcatcca tcgtcgttcc gaacacaaat gcctcccgtt tcccggcgca
     1381 tttcgcgcct cgacaacgca gggcgatcac cgcccctgcg ttcgctcacc cggccgcccg
     1441 ctctgcttct tccagggcaa tgcggaccca ttcctccatc cggcccggca cgttattgca
     1501 tgtgtgagtg tctttcagaa tcatctcaac cacacaaccg cgcgtgattt ccagcgtttc
     1561 gcgaatgctc gcccgaatag tttgcggatc gtagggattg gatacacaga ccggattcgg
     1621 tttccaggaa aacacatact tgttctggag tttttcggct gcaaccctca catccgtcca
     1681 cggcgagatg gagattcttc ggagtttcgg aatgtgctga atgacaagat ccagtttgtc
     1741 ggtcaaggat tcgcagcatc cgtagcaatt aaggccaaag cgttcaagga ggggcgcctg
     1801 gtacttgatt ccgaactcgt agtacatgcg gggagaaacc agcgcatact cctgcgattc
     1861 ggcgaacccc cacgtgtcct tgaggcgcgc ttttcctgcg taatcgctcg ctgggagctg
     1921 cctcgtgtag catgtccctc cggaacccgc gtaatcggca ccgtccataa gttccaactg
     1981 gccggacgcc tcgagttcgt ccagaagaac gcgggtattc tcaaggagaa atgaaaatac
     2041 ctcgtgcaac cagtcgggac gatccaccat gtcgaccatc acctgctcca gtccgcgcag
     2101 gtagcacacg aagttgacga ggctcgtgtt aatccccggc gaccgtttcc aacggatgtg
     2161 caagatatct ccaaacgcat cgtacaatcg actgtagttg cgcttgttca cctcgtgatt
     2221 caccacaaga tgcgggcgcc gcatcttggc aaagtcctcc ggagtcttca tcaccgggtc
     2281 ataggctcgc gctccgcgct cggtggttgt gggcttcatt cgagtttcca ggccccatcc
     2341 ggcggcgccc cgggaagggg aaagctcaaa cacggggtct atcacgttgt cgtcgcgcag
     2401 ccgctccccg cggtaaatgg tgtaccgtaa ccaccattca acagagcgcc agaagggatc
     2461 ctccaacttc atcgtggagt cgggcaacag ttccgaccac gccccctcag ggaaaatcag
     2521 caccatcgga cgcctcgggt tcagatcgtt gaaatcgtac cacatttccc gacgctcggc
     2581 gtagtacggg tctgcggcga tttccgccac acgttgcgcc agtccgcgta ggtactgctt
     2641 gtctgaggtt gtcaaaccca ttagaagcct cctgagtaaa cgcggtatta atgtacgcgc
     2701 cccgggccag gcgacccacg ccccgcgttg cccggcatca cattccgttt cgctcgtccg
     2761 gcaggctcaa cgtatctttc gatagagcag aagcaagatg gaggaactga acacatgcag
     2821 ggtccccgac agattccgcc cggccgccgc gggttccgta ccaggcagct ggacgggtta
     2881 tcccgtgtgc gcccagaagc cggcgaagtg ccccctttgc cgcgtgcaca gagggtttct
     2941 cgcgtgagaa cgccgtccag gggcgaggag acgccacttc ccatacgtcg tgaaacgcca
     3001 actcactgga tctatcttca cgggtttggt tcagacccga tgtcgccaaa agcgcagttt
     3061 ttcaatcagc agttccgcgc cgccggtctg cccatcgtgg tgcccgacct caatgttcct
     3121 tcgtttgaac agctcaccat cacggcatgc ctcgccgagg ttgagcgtgt catttccgga
     3181 ttcccgagcg aagcaaaagt cggaatcgtc ggttccagcc tgggagggct catcgctctg
     3241 tttgcagcag accggtgcaa aaacgtctcc tacgttatgc ttctcgctcc cgcgctgggg
     3301 ctttttcgcg tcaattttgt cgggcttggg aaaccgggtg tgcgtgcgtg ggagaaaaac
     3361 ggttttctgg aagtcttcca ctctccctcc aacaggaatc gccggctctc cgcggacttt
     3421 gtcgcggatg ctcgccagta cgacgagcgt aatctccggc tatccatccc tgtaacaatt
     3481 gtccacgggc aacgagatga tattgtggat ccgcacctca gcattacgta tgcgcgcgcg
     3541 catcacaacg tgaagctgca cctcgttgac gacgatcaca gtcttcttgc aagcatcaca
     3601 cagatctggg aatggttgcg gatggatact cgtccacggc caaaccgcga caacgcttcg
     3661 gcgcactgat tctcagaggg agtcttcgca cacacgtttc tgaatcgcga ggagttctgc
     3721 gatacccttt ttccccagcg tgatcatctg cgcaagctga tcatctgtga acgggactga
     3781 ttcggcggtt ccctggagtt ccacgaagca cccggtgcca gtcatcacaa ggttcaggtc
     3841 cacgtcggcg cgagagtcct cttcataaca caggtccagc accggtaacc catctgatat
     3901 cccgacggag acagcagcca cctgatccag cagcgggtcc ttccgaatca tgtttttctc
     3961 gctcatccag cggacagcat ccgccattgc gatccacgaa cccgttatcg aagcggtccg
     4021 ggtcccgccg tcagcctgaa gcacgtcgca gtccagaatg atctgccgtt ccccgaggca
     4081 ggcaaggtcg atcactgcac gaagggaacg tcctatcaat cgctgtatct cgtgcgttcg
     4141 tccttttatg cgcccggttg cgctttctct ggggatgcga atgctgctgg aacgcggaag
     4201 catgccgtat tctgcggtca cccagccgtt cccctgaccg cgcaggaatg ctggtacaga
     4261 ttcctccacg gtggcagtgc acatcacgtg cgtgcctccg atttctatga ggaccgatcc
     4321 ctcagcatag ggagacacac ctctcgtgat tttgacgggt cgcatttcat cgggttgtct
     4381 tccgtcgggg cggttcggca ttgcatacct ccagtgactg agcaggacac gaaatcgtcc
     4441 cgtgacttcg aacggtgaga gacaggcgtc actcgaagtg aaccacgact cccgccgtgg
     4501 tccaccgtgg acggatggca acagtgccga aaaccgccca acgctcggcc ggggcaaacg
     4561 ggatgatgtt cccgggcgac cacctcccgt ttccgtcctc gtcgtaccag gcaaacacct
     4621 cgtaatcacc cgcaggcaac ccattcgcgt gccatgacag agaatcggag accacggcgg
     4681 ggaaaattgg cccttcctcc gtttttcgta acaacacgcg aagattcctg ccgggaatag
     4741 agtcgcttac tacgccggaa acctcgccca ttgagtcctc gcgagcgggg tacagcgtca
     4801 cacggactga gtcctcgcac ggatttccgc gatcgtctct cagatctgcc aacggaacca
     4861 cccagttcct ctctctagag cccatcagcg tgtttgctgc gaacatgaag tggttggcgt
     4921 gtctctgatg ccacgctcct tcgacaatcg tgccatcgtc ctgaaaagcg cgcccgaatc
     4981 cagtcgccgg cacggcaagg tcgtttgtcc acacggaaaa accggcggga gagtcgggag
     5041 aaatggcacg gttatcctcg acggattccg cgcgcagcct catcgtcaca gcctggccgg
     5101 acgcgacgaa agacgttgta tcggcagccc attccgccgg gttccccgct cggtcccatg
     5161 cacgcacgtg agcaccgtac gccgcccccg gtttcaaccc gctgcagtaa actacgactc
     5221 tgtttgacgc acgtggcgtc ttcagtactg agcactcgat cggtgtggca ccggacgcag
     5281 gctcaaccac ctccatacgc aaggaatcca ccggctcacc gaaaagcatc tccacgtgtc
     5341 tggcgtcgag cgcgcgaatt cgttccagcc gcggccctgt gcggtccaaa agccccatgc
     5401 agaccgaagg aatccttacg tgggcgccat ccttgaccac cacgccagtc gacgcgagcc
     5461 ccaccggttc ctctgcatcg atcagaccgt cactattcgc atcgcgccag gcaaacaggc
     5521 ggtacgtacc atccggcaaa taaccgattg agaagcgacc ggcgtttccc gactgcgtca
     5581 cgtaggcagc gtattcgcgc gccggatccg ggctggggtt atcttgcagt ggaaaaaggc
     5641 cgatggtaag ccctgaagtc ggcacgaagc gaccgtcata caccactccc tgcacgcgtc
     5701 cccggtgaat gctgtcgccc gtggaaaacg cgaaggcctg cgcggaagca agcctgacgc
     5761 cgtgcaggtc tgtgactcct gttccgatgg ttatcacata cgttttctgt ggttccagac
     5821 caccctcgaa tcgaatcgtg agaacccggt cgccccgtga ccattcgaaa cgcggatacc
     5881 gaacgggata gggtgtgata aagatggcct gatcggcaga agctttgttc attcgctccg
     5941 agaattcgat ccggggattc atgtccaaag agacacgcgt ggcgctgtct tccggtgcca
     6001 ccctcacaac acgtggcggg attgtatcct ccggtccccc tcccggagaa cccacgcggg
     6061 cgcacgccgc acaccacaac gcaagacagg gaacgattcg gaggtatacc cgggcactaa
     6121 tcgccaaccg attccccacc caacaggaaa agcaaaacag ctttctggac atggagtcgg
     6181 ttctccgcct cgtcgaacgc gatacagcgg gcagaatcca gcacctcgct cgtgatctct
     6241 tcaccccggt gggcgggcag gcaatgcgat accaccgcgt gttcaggcgc actcctcaac
     6301 agggctccgt tgacctgata ccgggagaac gctgcgcgcc tcgtctccgt ctccgcctcc
     6361 tgtcccatac tggcccacac gtctgtgtag atcacgtcgg cgtcgcgcac ggcggtttca
     6421 ggttcatcta caatcgcgca cgtgccgcgc agttcgcggc acttttcccg aataacctcg
     6481 gatggttcat accccgccgg gcaactgatc gtgaaacgca tccctgtaat ggcacataac
     6541 aacatgatcg aatgcgctac attgtttccg tcaccgacaa agacaacgtg cgttccgcga
     6601 agctctctcc tgtctcgtat cgccatggca agcgcaagtg cctgacaggg gtgctcgagg
     6661 tcgctgaggg cgttgatcac gggaaccgcc gcatgttccg ccagtcccgc gaccgtgtca
     6721 tgcgagtacg ttctcgccat gatgccctgc acccagcggg aaaggtttcg cgcgacatcg
     6781 gcgaccggtt cgcgttttcc gatctggatg tcacccggcg cgaggtaaat cgcgtggcca
     6841 ccgagttgcg tcattccact ttcaaacgtc accctggttc gcagagatgg cttctcaaaa
     6901 accatcgcca acgttttgga ggcgagcgtg tgtcgtggaa tcccagatcg ccattccacc
     6961 cgcagcttcc gggccagatc gatcagtccg ttgatctctt tttcggagaa gtcgctcatc
     7021 gagacaagat gccgtactgg tttcatgatc attccctata gcacatagcg gctgaggtta
     7081 tcgtcctgca gaatggaacg gagtcttgtt tccacccgtt ccggggtgat gactacctcc
     7141 cgaatctgct cgtccggaac cgcaaacaga atgtcgtcaa gaagaaccgt catcacggtg
     7201 tggaggcgcc gggcacctat gttttccgct cgtgagttca cttcgttcgc tacttccgcg
     7261 attttccgga tcgcttcagg cacaaactcg accctcacgc cttccgtctc cagaagagca
     7321 gtgtactgct tcacgagagc attctgcggt tccgtgagga ttcggacgaa atcgtcggca
     7381 ccaagactat ccagttcgac acggatcgga aagcgcccct ggagctccgg tatcagatcg
     7441 cccggcttcg ccacatggaa cgcaccggca gcgatgaaaa gaacgtggtc cgtccgcacg
     7501 gcgccatgtt tcgtactgac ggtggtgcct tccacgacgg ggaggagatc gcgctgtacc
     7561 ccctcacggg aaacatctgg accccccttg ccctcacggc ccgcaacttt gtcgagttcg
     7621 tcgatgaata ttatcccgag ttcttccgcg cgacgaatgg cctcgtttgt tacgtcctcg
     7681 ctgtcaataa gcttcccggc ttcttcctgc tccagtattc tgagcgcttc cctcactttc
     7741 actttccgcc gcttcgttcg ccccggaaac atgttgccga acacactccc caggttcatc
     7801 cccatctctt ccataccgcc ctgcggggta aaaacctcaa ggagcggcat tctgttctgc
     7861 ggcaaatcca gttccactat ccgctcgtcg aactcacccg cttcaagttt ggctcggagc
     7921 cgccgtcgaa cccgttcccg cgctccgtcg tcagtttcgg cggtgggttg ttcatcccct
     7981 gaccccggcg gaagcaaaac atccagcagg cggtcttgtg cggctctgcg ggctggttct
     8041 cgaaccgact ccagtcgttc cgaacgcacg aggccaattg cagcctcggc aaggtcgcgg
     8101 accatggatt caacatcccg cccgacatac ccgacctcgg tgaatttaga ggcctcgact
     8161 tttacgaacg gtgcaccggc aagcgccgcg agacgcctcg cgatttccgt tttgccaacc
     8221 cctgtggggc caatcatcag aatgttgttc ggcgttattt cgttgcgaag attcccttcc
     8281 acgcgttgac gtcgccatct gtttcgtaac gcgatcgcca ccgccctttt tgctttttgc
     8341 tgccccacaa tgaatcgatc gagatacgca acaacctgcg ccggtgtgaa cgctccagca
     8401 actgcaggaa caggcatatc agcaatcgcg tctcccgcga attccaacgc aaatcccctc
     8461 tcttccgagc acactccaat cgcgacccga gtacagcacg acaagaagat acctaaccgc
     8521 ggtacgccgg gaaacgcccg tgggttgaca cgctactgca tgtaccgctc attcagagtc
     8581 cgtttgagca acttgacgcg atccaattcc caggtgaatg catggctatc agacttgttg
     8641 cgatagatat cgaccgtact cttctgaacg accggagaga gctttcaccc gcgaacgaga
     8701 gtgccctccg ggatctcgtg gagcgcggga tttccgtggc gttgtgcagc ggaagggacc
     8761 tcccctcaac gcaagccata tctgctccac tcggtttgcc gctgtggttg gtcgttcaaa
     8821 acgggtcgct cgtaatcgat ccgtcgggga acgcggccta tctttgttcc atggaaaagc
     8881 cgacggcgct cgccgtgctt gatatcctcg aacgccacgg gcttgcaccg gtggtgtacg
     8941 aggtgtaccc gcgtgcgcat tgtctgtggt ggcagaccgg ggctcgagcg gctccgggga
     9001 tgattgaatt tcgcacgcaa cacggcgccc aggtatcgca tgttgaagac attcgttcag
     9061 ttcttacgcc ggaggtttct cacctcgaag tcttcgatga cgcagaccgt gttgtgcgtg
     9121 cgggtcagga tttcgccacg ttggatgaag tggtggcgat cacgaacatg agctcttcgg
     9181 ttgtcggtag cgctctgatg ggattgtacc cccgtggaac tgccaaagag gaagctttgg
     9241 cgcgcgtctg cgatgttctt cggatttcgc gaactgaggt cctcgcaatc ggtgacaatc
     9301 tcaacgatgt tggcatggtc cggtgggcgg ggattggcgt gatgatggca aacggccccg
     9361 aagaagcgcg cctcgaggcc gattgggtag ccccctcaaa taacgaaaat ggggttgcgg
     9421 ttgccgtgga acggttcacg aagtagtatg atccgcgcgg ttcaacagct cgtcatatag
     9481 cacgcggtat tgctcagcgg ccgcagacca ggaaaaatcc tggcgcatgc ccgcccgcat
     9541 gatcgactcc cacctctcgc ggtcgttgaa aaacttcatg gcgcggcgca cagcggtcag
     9601 gagcgcgtcc accgagtaac gcgagaaaga aaagccgttc cctcgctttg ttcgtccatc
     9661 atagttcgta accgtatctg caaggccgcc ggtggcgtgc acgatcggta ccgtgccgta
     9721 gcgcatgctg tacatctggg tgagcccgca cggttcgtat ttcgagggca tcaaaaacat
     9781 atcggcaccc gcctcgaccc gatgtgcgag c
//
LOCUS       DAOSOO010000112         9618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7070_length_9618_cov_5.547318, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000112 DAOSOO010000000
VERSION     DAOSOO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7070_length_9618_cov_5.547318"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLT86_08920"
     CDS             <1..622
                     /locus_tag="PLT86_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36774.1"
                     /translation="QGNPDAELEKRLVEIRTEIRRAESQAEAKELGVRESAFLELKGI
                     YYDHTISADDRNSAREHVLRLRDRHPTYSLLFLVAHRDDMHPVHKLSTQLIVDIAEQD
                     LRWASTAIWQYESPWITFSPHQVEYVVPFDKNAMETKVRAMSMHRSQEYRTRYSDVAR
                     YSAQMRAETFPELLGGFGQGQSGYQWDYIEAFQELRPVRVLHMSGD"
     gene            638..835
                     /locus_tag="PLT86_08925"
     CDS             638..835
                     /locus_tag="PLT86_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36775.1"
                     /translation="MALQKAREFTYRPPARRRAQIAGAEREKIRIPRAGNGKTGNASL
                     IRVVLMLGAVIALAIILSSFR"
     gene            965..2635
                     /locus_tag="PLT86_08930"
     CDS             965..2635
                     /locus_tag="PLT86_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT36776.1"
                     /translation="MLRRTTERYPAHVAMRMFRNGRYEELTYTDFSDRVDLLAAGLMR
                     LGIKPGAHVSMLGENRPEWGMAYLAVHRAGAVCVPLDSLQKVPEFRHNITDAEVEAVI
                     VSERFLDDILEVREKSGMPRLIICMDKTDREDVLVMSDVMKGELPSTWPEVKLTDLAA
                     IIYTSGTTGQSKGVMLTQRNIVEDAACTYQVLEFGSNDTFLSVLPLHHTFECTGGFLL
                     PLYAGASITYARSLKSRDIIDDIKNTGVTFMLGVPLLFEKMMEGIQRKLAQAPASKRI
                     LLAGLFSVEKLGRKFGLRLGYKLFKSLREKAGLATVRIFCSGGAALPPHVSIWYQSLG
                     FPLYQGYGLTETSPVSHVNLDPDYPASVGFPISCVETRIDRPGPDGHGEICLKGPTIM
                     AGYFKNEEATRAAIDPDGWFHTGDIGIEDADGRLYISGRQKNVIVTASGKNVYPEEVE
                     FHINQSPWVLESLVIGKPIANTTSEEVYAMVVPNKEYFDEQAQLRGQAFTEEEIEAKV
                     REEVEAAIANIADYKRPKRFEIRFEEFEKTSTKKIKRFLYKQHVISLS"
     gene            2660..3769
                     /gene="dgoD"
                     /locus_tag="PLT86_08935"
     CDS             2660..3769
                     /gene="dgoD"
                     /locus_tag="PLT86_08935"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HOT36777.1"
                     /translation="MKIVDVEPVPLAPRWLFVKVTTDEGIVGWGESLGDRALTIAAAI
                     TELKRYLVGKDPFQIEHHWQAMYRGAFWRGGPILNAAISGVDTALWDILGKVLGAPVW
                     QLMGGKCRDSVRFYRGIGGPSPEALAENALKAVEAGFSAVKWCPVPATPALAGVKGAK
                     AAEREVEAVRKAVGDGVDILLDFHGRLTPAMAIVFAKAVEPYNPMFIEEPVLPEHSEQ
                     IPRIAETTGVTIATGERIFTKFGFRPILQAGGVGLIQPDLSICGGLTEGKKIAAMADA
                     YAVGIGPHNPYGPVLTAASLQLDVCSPNFVIQEFVSLGEGLFTQPFEQKNGCVGVPYG
                     PGLGIEVDEEAVRAHEYVAHDVPILYNEDGSIADW"
     gene            3963..4976
                     /locus_tag="PLT86_08940"
     CDS             3963..4976
                     /locus_tag="PLT86_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT36778.1"
                     /translation="MRRRVFLWIGAASSILFLYIALRNIAFDQFLSAFKSVDVLPVSG
                     AFLILLLVYVVRTLRWRMIIESTERISNWRTFSTLMIGFFANNVLPARAGELIRAVIL
                     RRQIGVTRSFALGTIVVERVMDVAALVGLLLLAIYRVPPDRLPAITSEVRGFALGTLA
                     LVIAGMCVLLWGKDTVVALLESLFSKIMASHRAGILAGKFEQLAVGLEVMRDPSRLVF
                     VAFLSFLSWVGMAAVFYFVFVGFHLDVTPAVAAFTVSLVNLGMAVPSSPGYVGTYEFF
                     MVVSLGAFGIGKATALAYGLVTRLLWYVFEVVVGFACLWKSELSIRELIHGEESAIPG
                     DSG"
     gene            5139..8597
                     /gene="mfd"
                     /locus_tag="PLT86_08945"
     CDS             5139..8597
                     /gene="mfd"
                     /locus_tag="PLT86_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT36779.1"
                     /translation="MTLDQIRVRIAASDPAQALLRAISENRPEIPFHGASGSLPAFLL
                     AHLRKTSNRQILAVTPTTETAEELRDDLESILGEEAVRYFPDWELIAFEEKSPHVEVT
                     ALRLEALQSLLVGEPVVIVAPVVALLRPTLEPEALAAASRVIRVNELLDMDELAKWLR
                     SLSFEPSNQVQEMGQYARRGGIIDIFTFGSENPYRVELFDDEVESIREFDPATQRSVR
                     EVNEMEILPRREILAGSEWWGNAAERVKRAEDAYGVSLDYVRNKLEFGIHFEGIEFWC
                     PVLTGTKPSVLDHLDSRALVFLSDPESITEQAEKFARETARLVSRRIERGEPTLPPDA
                     VFHSWQNLRETIAPRCLVSNHSLSPPGAVASFGAQSARRYDSSLEAVRADVDTFAERG
                     FSVVLFAENESQARRLEDLLGSSMDRIAVEIGKLHGGFVYPSAKLFVVNDHELFSRYQ
                     GRHRFRKFRGATPVTDVFALGPGDLVVHEEHGIGVYQGLERITVDGVSRDCLRILYRD
                     QDRLFVPADQINRVQRYSQDDSARPVLSKLGGSDWDRVKERTRKGVLKLAQELIELYA
                     IRQSRPGFAFSPDSTLMEEMEAAFIYEETRDQLRAISQIKGFMESPVPMDLLLCGDVG
                     YGKTEVAIRAAFKAACDGKQVAVLVPTTILAQQHFRTFSERLAGFPVKVDVLSRFRSK
                     HETEETLYQLKRGAIDIVIGTHRLLSDDVEFRDLGLLVIDEEHRFGVRHKERLKHMRQ
                     TVDVMSMTATPIPRTLYLSLMGARDMAVINTPPRDRLPVHTEVIPFDEDRISEAILRE
                     VARSGQVFFVHNRVQTIEERAEFLRELLPELQFRVAHGQMSEHQLEKVMLDFLEHRFD
                     CLVTTMIIQSGLDMPNVNTILVDRSDQFGLAELYQLRGRVGRSNHKAFAYLMVPKDRK
                     LPPESRRRLRAIEEFSDLGSGFKVAMRDLEIRGAGNLLGNEQSGFIAAIGFEMYVKLL
                     DDAVKELKGEIPERTEDCEVDVHVSAFLPDTYVGDGEQKMTLYRRLAESQTLAEVNEF
                     TDELRDRFGRPPEPASALIDLMKVKVAGRILGARRIAVDKHGKIRIVLGDGRKMTSTQ
                     IGDLVRRCPEPVEFKSNGDLVMEIQSGEPGGPAQAARVAAILESLIEDDASEAKKPVR
                     LVWGER"
     gene            8603..9490
                     /locus_tag="PLT86_08950"
     CDS             8603..9490
                     /locus_tag="PLT86_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT36780.1"
                     /translation="MTARLVPGAWLIAAGILAGCGWFQSQDGEPVAVVGDVKLTQGDL
                     DRMLANDAFEEKSVEARRRAVTRWVNRELLYQEALRRECDRDPIVRFQLETARRDILV
                     NALIDRSQRDSPVTEEQITRFYEENRNIFKRTEAMIHVRQIVLEDPKEARRVASEVQR
                     RPETFAEVARARSSDPSSADGGDVGFVTARTAYSPEVWQVLQRLSDNEISRPIPIDAG
                     WIILQAVEHRPAGSIKSLDEVRSEVVNRLRAAERANLMAALAESLKLKEDVEVFADRI
                     VPAAGALRSPGSRTPVGAE"
BASE COUNT         2025 a         2529 c         3052 g         2012 t
ORIGIN      
        1 ccaagggaat cccgacgcgg aattagaaaa gaggctggtg gaaatccgga ccgagattcg
       61 tcgggcggag agccaggccg aagccaagga actcggcgtc agggaaagcg cattccttga
      121 gctgaaaggg atttactacg atcacactat ttctgcggat gatcggaatt ccgcccggga
      181 gcacgtgttg cgtcttcggg accgacatcc cacctactcg ctgcttttcc tggtcgcgca
      241 ccgggacgac atgcatccgg ttcacaagct ctccacacag ctgatcgttg atatcgcgga
      301 acaggacctg cgttgggctt ccaccgccat atggcagtac gaaagcccct ggataacgtt
      361 ttctccgcat caggttgagt acgtagtgcc gtttgacaaa aacgcgatgg aaaccaaggt
      421 acgggcgatg tcgatgcatc gatcgcagga gtatcgaacg cgctactcgg acgttgcgcg
      481 atacagcgct cagatgaggg cggaaacgtt cccggagctg ctgggcggat ttggccaggg
      541 tcaatcaggg taccaatggg actacattga agcgtttcag gagcttcggc cggtaagggt
      601 gctccacatg tcgggcgact gagaaggcgc aggcgaaatg gctttgcaga aagcgcggga
      661 gttcacctac cgtcccccgg cgcgcagacg cgcccagatc gcgggtgcgg agcgagagaa
      721 gatcaggata ccacgggcag ggaatggcaa aacgggcaac gcatcgctga tccgcgtggt
      781 cttgatgctc ggcgccgtta tcgcgttggc gatcatcctt tcatccttcc gttaggcgca
      841 gggtcgcccc aaggcgccct gcgaagtcgg tgaaggctgc tttcctcagg aggtgaaatc
      901 tatggctggc tccggacgaa aaatcgctgt gcgcgaccac ctaggcgtct gtaccacagt
      961 ggagatgctg cgccgaacta cagaaagata tcccgcacac gtggcgatga ggatgtttcg
     1021 taacggaagg tacgaggaac tgacgtacac cgatttcagc gaccgtgtgg accttcttgc
     1081 cgcagggctc atgcgcctgg ggatcaagcc cggagcacac gtgagcatgc tgggtgaaaa
     1141 tcgtccggaa tggggaatgg cgtacctcgc ggtgcaccgc gccggtgcgg tctgtgtgcc
     1201 tctggacagc ctccagaagg ttccggaatt ccgccacaac atcaccgatg cggaggtcga
     1261 ggcagtcatt gtttccgaac gcttcctgga cgacatcctg gaagtgcgtg agaagtctgg
     1321 gatgccccgc ctgatcatct gcatggacaa aaccgaccgg gaagacgtcc tcgtgatgag
     1381 cgacgtgatg aagggcgaac tgccgagtac gtggcccgaa gtgaaactca ccgatcttgc
     1441 ggccatcatc tatacttcgg ggacaacggg gcagtcaaag ggcgtgatgc tgacccagcg
     1501 caacatcgtg gaggatgccg catgcaccta ccaggtgctc gaattcggct cgaatgatac
     1561 gtttctttca gtgcttcctc tgcaccacac atttgagtgt accggagggt tcctgctccc
     1621 gctctatgcg ggcgcttcca tcacctatgc gaggagcctg aaatcccggg atatcattga
     1681 cgacatcaag aatacgggcg tcacgttcat gctgggagtt ccgctgctct tcgagaagat
     1741 gatggaaggg atccagcgga agcttgcgca ggcgccggct tcgaaaagga ttctgctcgc
     1801 gggtctgttc agtgtggaaa aacttggccg taagttcggc ctccggttgg ggtacaagct
     1861 cttcaaaagc ctccgcgaaa aggcagggct cgccacggtg agaatattct gctcgggcgg
     1921 cgctgcgttg cctccacacg tgtcaatctg gtatcagagc ctcggttttc cgttgtacca
     1981 ggggtacggg cttacggaga cgtcaccggt atctcacgtg aacctcgatc ccgattatcc
     2041 tgcgagcgtt ggttttccga tttcctgcgt ggagacgagg attgaccggc cggggccgga
     2101 cgggcacggg gaaatatgcc tcaaagggcc gacgatcatg gcgggttatt tcaaaaacga
     2161 agaggccacc cgtgcggcga tagacccgga cggatggttc cacacgggcg atatcggtat
     2221 tgaggatgct gatggacggc tttatatcag cggcaggcag aagaacgtga ttgtcactgc
     2281 gagtgggaag aatgtgtatc ccgaagaggt tgagttccac atcaaccaga gtccgtgggt
     2341 gctcgaatcg cttgtgatag ggaaaccgat cgccaatacc accagcgaag aggtgtatgc
     2401 gatggtggta ccgaacaagg agtacttcga cgagcaggcg cagctccgcg ggcaggcttt
     2461 cactgaagaa gagatcgaag ccaaagtccg ggaagaagtg gaagccgcca ttgccaatat
     2521 cgccgactac aagcgaccga aaagattcga aatccgtttc gaggagttcg aaaagacttc
     2581 cacgaagaaa atcaagcggt tcctgtacaa acagcacgtg atttcgcttt catgagatcc
     2641 tccccgggag ggtgagcgaa tgaagatagt cgacgtcgaa ccggtgcccc ttgcgccacg
     2701 ctggttgttt gtcaaagtaa ccacggacga agggatcgtc gggtggggtg aatcgcttgg
     2761 cgatcgagcg cttacgatcg ccgctgctat caccgagttg aaacggtatc tcgtgggcaa
     2821 agaccctttc cagattgagc accactggca ggcaatgtac cgaggtgcct tctggcgcgg
     2881 tggccctatc ctcaatgccg cgatcagtgg agtggatacc gcactctggg acattcttgg
     2941 caaagtgctg ggcgctccgg tctggcaact catgggtggg aagtgtcggg attcagtgcg
     3001 gttttatcgc ggaataggcg ggccttcacc ggaagcactg gcggagaatg cactcaaggc
     3061 ggttgaggcc ggtttctcgg cagtgaaatg gtgtccggtt cccgccacgc ccgcgcttgc
     3121 gggcgtcaaa ggggccaaag ccgccgagag ggaggttgaa gcagttcgga aggcagtagg
     3181 ggacggtgtt gacattctac tggatttcca cggccgactc acccctgcga tggcgattgt
     3241 gttcgcgaaa gcagttgagc cctacaatcc catgttcatc gaggaaccag tgcttcccga
     3301 gcactccgaa cagataccga ggattgcaga aaccacgggt gttacgattg ctaccggtga
     3361 gcgcattttc acgaagttcg ggttccgtcc catccttcag gctggtggag tgggtcttat
     3421 ccaacccgac ctgagcattt gtggcggtct gacggagggc aagaagattg cggccatggc
     3481 agatgcgtac gccgtcggca taggcccgca caacccgtac ggtcccgttc tgaccgcggc
     3541 ttccctgcaa ctggacgtct gctcgcccaa tttcgtcatt caggagtttg tcagccttgg
     3601 tgaagggctg ttcacgcaac cgtttgagca gaagaatggc tgcgtcgggg ttccttacgg
     3661 gcctggactg ggcatcgagg tggacgagga ggccgtgcgg gcgcacgagt atgtggccca
     3721 tgatgtgccg attctttaca acgaggatgg cagcatcgcc gactggtgac ggggcactgc
     3781 ctgaccgacg tgaaaaaccg atctcgctgc cgtaccccgg cagccgatga ttcgtgccgc
     3841 gcctgtgaac aacaacgtgt tgttggacgg gcgcggtttc cttcatgatt cctgttgaag
     3901 gcggcaggac cttcgcccgt cagctttgcc tgccagaacg ccgccatcga agcggaggtt
     3961 gagtgcgcag aagggtcttc ctgtggatcg gagcggcgtc cagcattttg tttttgtaca
     4021 tcgctctgag aaacatcgcg ttcgaccagt tcctcagcgc gttcaagagc gtggacgtat
     4081 tgccggtttc tggcgcgttt ttgatcctgc tccttgtgta cgtagtgcgc acgttgcgat
     4141 ggaggatgat cattgaatcc accgaaagaa tcagcaactg gcgtacgttc agcactctga
     4201 tgatcggttt tttcgcgaac aacgtccttc ccgcacgagc cggggagttg atccgcgcag
     4261 tcattttgcg gcgtcagatc ggtgtgacga gaagcttcgc cctcggcacg atcgtggtgg
     4321 aacgggtgat ggacgttgcg gcccttgtgg gattgctttt gctcgccatc taccgggttc
     4381 ctccagatcg cctgcccgcg atcacttcgg aagtgcgagg gttcgcgctg ggaacgctgg
     4441 cgctcgtcat cgcggggatg tgcgttcttc tgtgggggaa ggacacggtg gttgcgttgc
     4501 tcgagagctt gttctcgaag atcatggcgt cccacagggc agggatactg gcgggcaaat
     4561 tcgagcaact ggccgttgga ctggaagtga tgcgcgaccc tagtcgtctg gtgttcgtcg
     4621 cgtttctgtc gttcctaagc tgggttggga tggctgcggt tttctacttc gtgtttgtcg
     4681 gattccatct tgacgtgaca ccggcagtgg cagcgttcac tgtctcgttg gtaaaccttg
     4741 gaatggccgt gccttcgtct ccggggtacg ttggtacgta cgagtttttc atggtggtgt
     4801 cgcttggtgc gttcgggatc ggaaaggcga cagcactggc gtacggcctg gtgactcggt
     4861 tgctatggta tgtgttcgag gtcgtagtgg gttttgcgtg tctttggaaa tcagaactgt
     4921 cgattcggga gcttattcac ggtgaagagt ccgcgattcc cggcgattca ggttgaaaag
     4981 aagctgcgcc gcttggactt ctgagtcgga taagtttcct tatgatacgt gatccccggg
     5041 ttttttcggg ggcactttcg cgatcgagaa gccgatgatc atggacgatc accggcgttt
     5101 tgctgtttat gtccgcccgc ttgaaccgaa ggaccgcttt gacgcttgat cagatacgag
     5161 ttcgcatcgc tgcaagcgat ccagcccagg cgctgctccg agccatttcc gagaaccgcc
     5221 ctgaaatccc ttttcacggc gcatctggtt cgcttcccgc gtttttgctc gcgcacctca
     5281 gaaagacgag taatcgccag atccttgcgg taacgccgac aaccgaaacc gcggaggaat
     5341 tgcgagacga cctcgagtcg attctgggcg aagaagcggt acggtatttc cctgactggg
     5401 aactcatcgc attcgaggaa aagtctcccc atgtcgaagt aaccgcgctc cgactcgaag
     5461 cgttgcagtc gcttctcgtc ggcgaaccgg tggtcatagt agccccggtc gtggccctct
     5521 tgcgtccgac actggaacct gaagccctcg ccgccgcgtc tcgcgtcatt cgagtgaatg
     5581 agctgcttga catggacgaa ctcgcgaagt ggttgcggag cctcagtttt gaaccttcga
     5641 accaggttca ggaaatgggt caatatgcac gccgcggggg catcatcgac atcttcacct
     5701 tcggctctga aaacccgtac cgtgtcgagc ttttcgatga cgaagtggaa tccatccggg
     5761 aattcgaccc ggcgacccag cgttccgtgc gcgaagtcaa cgaaatggag atcctgccga
     5821 ggcgggagat ccttgcggga agcgaatggt ggggaaacgc cgccgaacgt gtgaagcgag
     5881 cggaagatgc ctacggtgtg agtctggatt acgtcagaaa caagcttgag ttcgggattc
     5941 attttgaagg gattgagttc tggtgccctg tattgacggg cacaaaaccg agcgtgctgg
     6001 accatctcga ttcccgtgcg ctggtgttcc tgtcggaccc tgaaagcatt acggaacagg
     6061 ctgaaaaatt cgccagggaa actgcgaggc tggtcagtcg gaggattgag cggggggaac
     6121 caaccctgcc tcccgacgca gtgtttcaca gttggcagaa tctgcgggaa acgatagcgc
     6181 cccgatgtct ggtatccaac cattcacttt cgcctccggg ggcagtggcc agtttcggtg
     6241 cgcaaagtgc acgacggtat gactccagtc tcgaagcagt gcgcgccgac gtcgatacct
     6301 tcgcggagcg cgggttttcg gtcgtcttgt ttgcggagaa cgagagtcag gcacggagac
     6361 tggaggacct gctgggttct tcgatggatc gcattgccgt ggagatcggc aaactccacg
     6421 gcgggttcgt gtatccatcc gccaagctgt tcgtggtgaa cgatcacgag ttgttcagca
     6481 ggtatcaggg acggcacaga ttcaggaagt ttcgcggcgc aacacccgta acggacgtgt
     6541 tcgcgctcgg tccgggcgac ctggtggtgc acgaagaaca cgggataggc gtgtaccagg
     6601 ggctcgaacg gatcacggtg gacggcgttt cccgggattg cctgcggatt ctttaccgcg
     6661 accaggaccg ccttttcgtc ccggcggacc agatcaaccg tgtccagcgg tattcgcaag
     6721 acgacagtgc gcgcccggtc ctttccaaac tgggcggttc tgactgggac cgggtcaagg
     6781 agagaacccg caagggtgtt ttgaaactgg cgcaggagct cattgagctg tacgcaattc
     6841 gccagtcccg cccggggttt gcgttcagcc ccgattccac gctcatggag gagatggagg
     6901 ccgcattcat ctacgaggag acccgcgatc agctacgcgc gatctcgcag atcaaaggat
     6961 tcatggaaag ccccgtgccg atggatctcc tcctttgtgg ggacgtcggg tacggcaaaa
     7021 ccgaggtagc cataagggcg gctttcaaag cggcgtgcga cggcaaacag gtcgccgttc
     7081 ttgtaccgac caccatcctc gcgcagcaac actttcgaac attctcggag cgcctcgccg
     7141 ggttcccggt caaagtcgat gtgctgagcc gttttcgttc caaacacgaa acggaagaga
     7201 ctctgtacca gttgaaacgg ggtgcaattg acatcgtgat cggtacgcac cgcctcctct
     7261 ctgatgacgt tgagtttcgt gatctcggtc tgctggtgat cgacgaggaa caccgcttcg
     7321 gcgtacgcca taaggaacgt ctgaaacaca tgaggcagac tgtagacgtc atgagcatga
     7381 cggccacgcc gattcctcgg actctctacc tgtcgctgat gggtgcgcgg gacatggcgg
     7441 taatcaacac gccaccgaga gacaggttgc ctgttcacac cgaggtgatt ccgttcgacg
     7501 aggatcggat atcggaggca atactcagag aagtcgcgcg ctccggacag gtttttttcg
     7561 tccacaaccg ggtgcagacg atcgaggaac gtgccgagtt tctacgagag ctgctgcctg
     7621 aattgcagtt ccgcgttgcc cacgggcaaa tgagcgagca ccaactggaa aaggtgatgc
     7681 tggacttcct ggagcaccgc ttcgattgcc tcgtcacgac catgatcatc cagtcaggat
     7741 tggatatgcc gaacgtcaat acgatcttgg ttgatcggtc agatcagttc ggtctcgccg
     7801 agttgtacca gttgcgagga cgcgtcggga ggagcaacca caaagccttc gcatacctga
     7861 tggttcccaa agatcgcaag ttgccgcccg aatcacgacg aaggctgcgc gcgatcgagg
     7921 agttcagcga cctgggcagc gggttcaaag tcgcgatgcg cgatttggag atccggggtg
     7981 cgggcaatct gctggggaac gaacaaagcg ggttcatagc agcgatcggt ttcgaaatgt
     8041 acgtgaagct gctggacgac gcggtgaagg agctgaaggg cgagattccg gaacgaacgg
     8101 aagattgcga agtagacgtg catgtcagcg cgtttctgcc cgacacgtac gtgggtgatg
     8161 gagagcagaa gatgaccctg taccggcgcc ttgcagaatc gcagactctc gcggaagtaa
     8221 acgagtttac cgatgaactg cgcgaccgtt tcggccgtcc tccggaacct gcaagcgccc
     8281 tgattgatct aatgaaggtc aaagttgcag gaaggatcct cggcgcacgc aggattgccg
     8341 tggataagca cgggaagatt cggattgtcc tcggagacgg caggaagatg acatcaacgc
     8401 aaatcgggga tttggtgcgc cgatgtcccg aaccggttga gttcaagtcc aacggtgatc
     8461 tggtcatgga gatacagtcg ggggagccgg gcggaccggc gcaagccgcg cgagttgccg
     8521 cgattctgga atccttgatc gaagacgacg cttccgaggc aaagaagccc gtgcgacttg
     8581 tctgggggga aagatgaggc ggatgacagc ccgtctggtg ccgggtgcgt ggctgatcgc
     8641 ggcgggaatc cttgcggggt gcggctggtt ccagtcgcaa gatggagaac ctgttgccgt
     8701 ggtgggggac gtgaagctca cacagggtga tttggacagg atgcttgcca atgacgcgtt
     8761 cgaggagaaa tcggtcgaag cacgaaggcg agccgttacg cgatgggtaa atcgcgagct
     8821 tctgtaccag gaagcgttgc ggagggaatg cgacagggac ccgattgtca ggttccagct
     8881 cgagacggca cggcgtgaca tcctggtgaa cgccctcatc gaccgttcgc aacgcgattc
     8941 cccggtcacc gaggaacaga tcacgaggtt ttacgaggaa aacaggaata tcttcaagcg
     9001 gactgaagcg atgatccatg tccggcagat cgtgttggaa gacccgaagg aggcgcgacg
     9061 cgtggcctcg gaagtccaac gcaggcccga gacgttcgca gaggttgccc gcgcgcgttc
     9121 cagcgatccg tcaagcgcgg acggaggcga cgtcggattt gtgacggctc gaaccgcata
     9181 cagccctgag gtgtggcagg tattgcagcg gctttccgac aacgagataa gccgcccgat
     9241 acctatagac gccgggtgga taattctgca ggcagtcgag caccggccgg caggcagcat
     9301 caaatcgctt gacgaagtcc ggtcagaggt ggtgaaccgc ttgcgggctg cagaacgagc
     9361 gaatctgatg gcagctctcg cggagagcct gaaactgaaa gaggatgtgg aggtgttcgc
     9421 agacaggatt gttccggcgg ccggcgcgtt gcgcagcccg ggttcgcgga ctcctgtcgg
     9481 agcggaatga acgagtcgtc acgtgttgag tgcttcggga atgcaatgat ccgaatcttc
     9541 cttgtggaaa ggttactctg tgccgaaaaa caggcctttg tgttgtgcgt tgctgctgat
     9601 cgtggcaagt ctgctgcc
//
LOCUS       DAOSOO010000113         9544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7130_length_9544_cov_5.819897, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000113 DAOSOO010000000
VERSION     DAOSOO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7130_length_9544_cov_5.819897"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLT86_08955"
     CDS             <1..355
                     /locus_tag="PLT86_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36781.1"
                     /translation="LRECAECRAEFELESRLVRTGSRAPVYDPPGDFAARVVERWQAG
                     AVPAMLSPAEIVQKAFADGLKAAYAPLGAAYMPVIRETWAALAGAHEAIREASGVVRG
                     VLKEVLWGRFVLKTT"
     gene            371..775
                     /locus_tag="PLT86_08960"
     CDS             371..775
                     /locus_tag="PLT86_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36782.1"
                     /translation="MFEALAAMSTGKVVILGITAVLVAGILFVLVVATAAILGPGRRA
                     VEGAMKAVPNLSGEEAPRRVPTSLKILGIGVMFLMVGLGVVIAAAIPSLTGWGMFGVI
                     LLAAGIGLLLFYLVVAKFEKMTPPPRSAEEAD"
     gene            783..920
                     /locus_tag="PLT86_08965"
     CDS             783..920
                     /locus_tag="PLT86_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36783.1"
                     /translation="MAQRALGTTAISLDGGGMVKELGRVCRITAGSRSVPARGERTRE
                     E"
     gene            989..1384
                     /locus_tag="PLT86_08970"
     CDS             989..1384
                     /locus_tag="PLT86_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36784.1"
                     /translation="MPYIDSIMGTNRGRLAVLFAGCMLVSLFIAAIALLAAPVAKRVF
                     AGVMCIIGKITKQEMPRDIQLSGRIVGTAALFLSAVACTIIIRLATVPRDGWHTIGVF
                     LLSVGSALIVSNSVITRLEKNESSTKGGN"
     gene            1386..1715
                     /locus_tag="PLT86_08975"
     CDS             1386..1715
                     /locus_tag="PLT86_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT36785.1"
                     /translation="MSDNRAVDYRFSTGLKRAVYEVAPEVALFLYGSAARGTREPDSD
                     YDVLVLSEHALSDEEEDAIRNAVYDFELENEIVVSLVFFARGEWDSPLMSATPFHANV
                     TREGIQL"
     gene            1847..2119
                     /locus_tag="PLT86_08980"
                     /pseudo
     CDS             1847..2119
                     /locus_tag="PLT86_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            2131..2544
                     /locus_tag="PLT86_08985"
     CDS             2131..2544
                     /locus_tag="PLT86_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36786.1"
                     /translation="MQDSLQIIMTSLEAMDTWKILVIGVLFLWAVKIVLGIFATGFTY
                     FALPWRRFIKGGSSGLTVDLPGNPAERMPVSVRILGGAITLLFVGVAILLCAALPLAS
                     PWVAVGIILAALGAGLLVFYGLVQREEKRAAKENR"
     gene            2633..2848
                     /locus_tag="PLT86_08990"
     CDS             2633..2848
                     /locus_tag="PLT86_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT36787.1"
                     /translation="MKKLYRSTTDRKIAGICGGLAEMYDVDPLAVRLIAIALLLVTGL
                     FPLTVVYLLAWWLVPRRESSFPQAPQA"
     gene            3074..4219
                     /gene="tgt"
                     /locus_tag="PLT86_08995"
     CDS             3074..4219
                     /gene="tgt"
                     /locus_tag="PLT86_08995"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT36788.1"
                     /translation="MHTLSFELSATDKNARAGIVTTRRGAFPTPMFMPVGTLGSVKTL
                     SQDDLRAAGAQIILGNTYHLLVRSDPSRIHRMGGLHGFMNWDHSILTDSGGFQVFSLG
                     DLNRVTEEGVVFRSHFDGSMYTLTPERSVEIQHQLGADIIMAFDECAPYPAPLAQVIS
                     AADRSFRWAVRSLAAHRKLSEGYRDTAPPALFGIAQGGTSLWLRREQARLLSGLDFPG
                     YAIGGLSVGEPKELMWAVLEEIVPLLPADKPRYLMGVGYPEDLVEAVARGVDMFDCVI
                     PTRNGRKGTVFTNTGRLILKSARFAEDTAPIEEDCPCEACRQYSRAYIRHLIRCDEIL
                     GMRLCTLHNVTFYLRLVRRMREEVVAGTFDQWRREFLTKYRLEEGNQ"
     gene            4266..4628
                     /gene="yajC"
                     /locus_tag="PLT86_09000"
     CDS             4266..4628
                     /gene="yajC"
                     /locus_tag="PLT86_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT36789.1"
                     /translation="MRVARLTIAGLMLTGGNAFAMGAQPSGGQAGSPWGMFVPLLLMF
                     VVLYFFMIRPQQKRQKEKEKMLEALKKGDRVLTLGGIYGDIQQVKDSVVVLRIAENVK
                     VEVQRSAISSVVESAASE"
     gene            4659..5177
                     /gene="def"
                     /locus_tag="PLT86_09005"
     CDS             4659..5177
                     /gene="def"
                     /locus_tag="PLT86_09005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT36790.1"
                     /translation="MALLPIRIYGDPVLRRVAEPVREFTPELRALAYDMIETMYAEPG
                     VGLAAPQVGVSIRMIVIDPTAGDQKGKAFALINPVVTRSGEHVVIEEGCLSVPDIRAD
                     VVRPERVSVDYQTIDGEPAHLDADDIVARIIQHEYDHLDGILFVDRISPVRRQLLRKQ
                     LKALAKSQGKRS"
     gene            5264..6208
                     /gene="fmt"
                     /locus_tag="PLT86_09010"
     CDS             5264..6208
                     /gene="fmt"
                     /locus_tag="PLT86_09010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT36791.1"
                     /translation="MKPLRIVFMGTPEFSVPSLEKLASGGHHVVAVVTKPDKPAGRGR
                     AIQPPPVKRAAERLGLPVIQPPSLRDTDTHSRLEALQPDLFVVVAFRVLPEELLRIPR
                     LGAINLHASLLPKYRGAAPIQRALINGERETGVTVFLLEPTVDTGRILRQRPIAIGDD
                     ETCGELAARLSVTGAEELAAAVDDLARGAAVMIPQNDAEATTAPKLTKEDGRIRWNER
                     AEAIRNRVRALNPSPGAYALWKGQPFGILRAAVEEAPAPGDEAPGTILQADDRHGVRV
                     ATGDGILRLDIVQPAGRRPMEGAAWVRGAHPQKGERFE"
     gene            6355..7452
                     /locus_tag="PLT86_09015"
     CDS             6355..7452
                     /locus_tag="PLT86_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36792.1"
                     /translation="MTPRQRVHAALNRLPADRIPIFMWYHPVTARRLAAELDIPVSEL
                     STVMGDDVRQTWVGNNHAMEGIVHERDGETHRDDWGIEWVKSGHFNQIRSSPLEHASR
                     EEALAYQYPYNCVDSLMANMVPLLRYSDRYFIGCDVSPCLFEMICRLRGMEQATLDLA
                     EDPDLARTMLAEAGAFSRHLAEEACRRFRLDWLWTGDDVGGQLAMIMSPSLWRELVKP
                     RLKEIFAAGKTGGIPVAYHSCGAVRDIIPDLIEIGLDVLNPIQCNCPGMDPASLKREF
                     GRKLTFMGGVDTQGLLPQATADEVFRVTRKLIEIMTEDGGGYILAASHTIPPETPSEN
                     IFAMYDAAGLSREAIADAAADARKRGAGLRS"
     gene            7602..8420
                     /locus_tag="PLT86_09020"
     CDS             7602..8420
                     /locus_tag="PLT86_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36793.1"
                     /translation="MELLRRALLPCVCGMICLAAAGCGRDKFGNALDKVLEQDTVTTI
                     GVIGVFGSPGNHHYVERDAALAAYLAKIADGKYTIITIDTAVANKVLSVVVQPTSNFA
                     DPKVVAKIGKAFGFKIAVIGEVMPADPEKLAVRAIHSLDEEEIAAGEIPFEAPSTALP
                     ANVRRQTPPPVALNTDIKRFFIGHINAEEVAVTLDVKNVLPSSSTEAEFLYTMNAPDS
                     RQDGKGKLYLMENIVELEGLGVGIIYVNADGKIVLESADNNLNSSWLFEEVANR"
     gene            8417..9358
                     /locus_tag="PLT86_09025"
     CDS             8417..9358
                     /locus_tag="PLT86_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36794.1"
                     /translation="MNPRILFFMLLFAFVVGCGGGGDEEADLESSDPAKRIAALQRQV
                     ERLKRNDRMKDEYIRTVTRNINEIESRLDSVTRGQVSVTRVMSSNPELRMSSSQTQNM
                     LDDINTLQTAMKRNKAQIQSLRQQVSSSRFKIAELEQLVTTLTNRVAEKDSQIAVLVS
                     RVSELEGTVSRQQATITQQTRTLEEQSRLSAEQQQTIERQQRQLGTGYYTYGTLDGLK
                     RNGLVTERGGFLGIGKETVIAPGYNDDPSKFTEFDIQNENRLVIPHTRSQSIQKILPD
                     RASTSYQLERAATGTTIRIINPGTFWRDKFLVIVLGG"
BASE COUNT         1951 a         2722 c         3010 g         1861 t
ORIGIN      
        1 cctccgggaa tgcgccgaat gccgggcgga attcgagctt gaatcgcgcc tcgtcaggac
       61 cggctcccgt gctccggtgt acgacccacc gggtgacttt gcagcccgtg tggtggagcg
      121 atggcaggcg ggagccgtgc cggcgatgct gagcccggcg gagatagtgc agaaagcgtt
      181 tgcagatggt ctcaaggctg cgtatgcccc tctgggtgcc gcgtacatgc ccgtgatccg
      241 tgaaacatgg gcagcgcttg caggagcgca cgaggcgatt cgggaagcct caggagtcgt
      301 acggggggtg ctgaaagagg ttctatgggg tcggttcgtg ctgaaaacga cgtagcgaaa
      361 ggattgaacc atgttcgaag cgctggcggc aatgagcacg gggaaagtgg tgattctcgg
      421 tattactgcc gttctggtgg ccgggatttt gttcgttctc gtggtcgcaa ccgcggccat
      481 cctcgggccc ggcagacgcg ccgtggaggg cgcgatgaaa gccgttccca acctcagcgg
      541 ggaggaggct ccgcgcaggg taccaacatc cctgaaaatc ctgggaatcg gtgtcatgtt
      601 cctcatggtg gggcttgggg tagtcatcgc cgcggcgata cccagtctca ccggatgggg
      661 gatgttcggc gtcatcctgc ttgccgccgg catagggttg ttgctgttct atcttgtcgt
      721 cgcgaagttt gagaaaatga ctccgccacc acgcagtgct gaagaagcag actgaggagc
      781 agatggcgca acgtgcgctg ggcactaccg caatctccct ggacggtggc ggaatggtga
      841 aagaactggg acgtgtctgc cgtatcactg ccggatcacg ttctgtgccg gcgcgcggcg
      901 agcgcacccg cgaggaatag ctcgagatgg cggtgaggca cgcaaaggaa actggcgaga
      961 acatggtttt tgtggagaga aacgggtgat gccctatatt gacagcatca tggggacgaa
     1021 cagaggcaga ctcgccgtgc tgtttgcagg ttgcatgctc gtaagcctgt tcatcgccgc
     1081 gatcgccctt ttggcggctc ccgtggcaaa acgcgttttc gccggcgtca tgtgtatcat
     1141 cggaaaaatc acgaagcagg aaatgccacg ggacatccag ctttcagggc gtatcgttgg
     1201 aacggccgcc ctttttctca gcgccgtggc gtgcacgatc atcatacgct tagcgaccgt
     1261 acctcgcgat ggatggcaca cgattggcgt tttcctgcta agcgtgggat ctgcgctcat
     1321 cgtctccaat tccgtcatca ccagactgga aaagaacgaa tcctccacga aaggaggaaa
     1381 ctgaggtgtc agacaaccgc gcggtagatt acaggttctc tacgggcctc aagcgcgcag
     1441 tttacgaagt ggctccggaa gtggcgctgt tcctttacgg tagcgccgcc cgcgggaccc
     1501 gcgaacccga ctctgattac gacgtgctgg ttctgtcgga gcacgcgctc tccgacgaag
     1561 aggaggacgc gatccggaac gcggtgtatg acttcgagct ggagaacgag atcgtggttt
     1621 ccctcgtctt ttttgcgcgc ggcgagtggg attctcctct gatgtcggcc acgccgttcc
     1681 atgccaacgt cacgcgggaa gggattcagc tatgaccccg gagcaggtca cgctcgtccg
     1741 ataccgcctc gaacgcgcga aagaggccct tggcgaagcc gagttgttac tggacaacga
     1801 ccacttgaat gcggcggtta accggctcta atacgcttgt ttctatgctg tgagtggcct
     1861 ccttctgact gagggccagt catctgcaaa acacagcggc gttcgggctc tgtttgacgc
     1921 aaactgggtt aagagcgggc gcgtcccaaa ggatgcgggt aggttctacc accggctttt
     1981 cgatttgcgc cagaaagggg attacgccga tctgacgaca ttcccaccgg acgacgttcg
     2041 ggaatggcag aagtcggccg catacttcgt ggaaactatc agtgcagtcg ttgaaagtcg
     2101 aatacgcagc aacgaatagg agttcgtacc gtgcaggatt cccttcaaat catcatgacc
     2161 tccctcgaag ccatggacac atggaagatt ctcgtcatcg gagtactgtt tctctgggca
     2221 gtgaagatcg tactcgggat attcgcgacg ggattcacgt atttcgcgct gccgtggcgc
     2281 agattcatca aagggggtag cagcggcctc acggtggacc tcccgggaaa ccctgctgaa
     2341 cgaatgccgg tttccgtccg tattctggga ggcgcgataa ccctgctctt cgtcggggtt
     2401 gcaatcctcc tgtgcgctgc cttgccgctg gcatcgccgt gggtcgccgt cggaatcatc
     2461 ctcgcggcgc tgggagcagg tctgctggtg ttctacgggc tggtgcaaag ggaagaaaag
     2521 cgcgcggcga aagaaaaccg ttaggaaatc gcggggccgg aaggccgacc acgccgtttc
     2581 cgcgggaata cagcgcgcgc atctccacgc acatgcatga ggaacaaaca ggatgaaaaa
     2641 gctgtaccgt tctaccaccg acaggaaaat cgccggaatc tgcggcggat tggcagagat
     2701 gtacgacgtg gacccgttgg ccgtcagact gattgcgata gcactgctcc tcgtcaccgg
     2761 cctcttcccg ctcacagtgg tgtacctcct tgcgtggtgg cttgtgcccc ggcgcgaatc
     2821 ctcgttcccg caggcgccac aggcctgagc gccgctgtca aagtcacgcg ttcggtttct
     2881 gtcgtgagca gggcccctca ggcggtggtt tcgtgccgga aaccgggcgt cggtcctgcg
     2941 atgggcaatc ccgcttcggc cggcttcttc gacgtccgcg tgtgccggca cacctcgcga
     3001 atcgtatatt tcccactgct tctgtccaga ttgctgcgac tctgtcgtat ccgccccgat
     3061 tccggcgtta cccgtgcaca cactttcctt cgaactctct gccaccgaca aaaatgcccg
     3121 cgcgggcatc gttacgacac ggcgcggcgc gttcccgacg ccgatgttca tgccggtcgg
     3181 cacgctggga agtgtcaaga cgctctcgca ggatgatctc cgtgccgccg gagctcagat
     3241 catcctcgga aacacatacc acttgctggt gcgctccgat ccttcccgta tccatcgcat
     3301 gggaggcctg cacggtttca tgaactggga ccacagcatc cttaccgaca gcggcggatt
     3361 ccaggtgttc agtcttggtg acctgaaccg ggtaacagaa gagggcgtcg tgtttcggag
     3421 ccactttgac ggctcaatgt ataccctgac accggaacgc tcggtggaga tccagcacca
     3481 gcttggtgcc gacatcatca tggcgtttga cgaatgcgcc ccgtacccgg ctccccttgc
     3541 gcaggtcatc tctgccgccg atcgctcgtt ccgatgggca gtccgttcgc tggcggcgca
     3601 ccggaagctc agcgaggggt acagggacac agcaccgccg gcattgttcg ggatcgcgca
     3661 gggagggact tcgctctggt tgagacggga gcaggcccgc cttctctccg ggcttgattt
     3721 ccccggctat gccatcgggg gtctgtcagt cggtgaacca aaggaactca tgtgggcggt
     3781 gctggaggag atagtaccgc tgctgcccgc cgacaaaccg cggtacctga tgggcgtcgg
     3841 atacccggag gaccttgtgg aagccgttgc ccgcggcgtg gacatgttcg actgcgtgat
     3901 tccgacgcgg aacgggagga aagggaccgt gttcacgaac acagggcggc tcattctaaa
     3961 atccgcgcgg ttcgcggagg acactgcccc gatagaggag gactgtccct gcgaggcctg
     4021 ccggcaatac tcgcgcgcct acatccgaca tctcattcgg tgtgacgaaa tcctcggaat
     4081 gaggctctgt accctccaca atgttacatt ttatctccgc ctggttcgcc ggatgagaga
     4141 agaggtcgtg gcgggaacgt ttgatcagtg gcgacgggaa ttccttacca aataccggct
     4201 cgaagagggg aaccagtagc gttgttgata ctcggaaaga tgccgcacaa ccaaggagac
     4261 actgcatgcg tgttgctcga ttgaccatcg caggattgat gctgaccgga ggaaacgcat
     4321 ttgccatggg tgcccagcct tccggagggc aggccggcag cccctggggt atgtttgttc
     4381 ccctgctgct catgttcgtg gttctgtact tcttcatgat ccgcccgcag cagaagcgcc
     4441 agaaggaaaa agaaaagatg ctcgaagcgc tgaagaaggg cgatcgcgta cttactcttg
     4501 gcggcatcta cggggatatc cagcaggtga aggacagtgt cgttgtgctc cggatcgccg
     4561 aaaacgtgaa ggtggaggtc cagcgctccg ccatttcctc tgtcgtggaa tccgccgcct
     4621 ccgagtagaa tcgcatcagc gttcaggaca tttccagcat ggccttactc ccgattcgca
     4681 tctacggtga tcctgtcctc cgccgcgtcg cggaaccggt acgcgaattc acgccggaac
     4741 tgcgggcgct ggcatacgac atgatcgaaa ccatgtatgc ggaaccgggg gtagggctgg
     4801 cggcgccgca ggtgggggtt tcgattcgca tgatcgtgat cgaccccacc gcgggagatc
     4861 agaaaggcaa agcttttgcg ctgatcaatc ccgtggttac gcggtcggga gagcacgttg
     4921 tcatcgagga agggtgcctg agcgtgcctg acatcagggc tgacgtggtg cgccccgagc
     4981 gggtttccgt ggactatcag acgattgacg gcgaaccggc gcacctggat gcggacgaca
     5041 tagtggcgcg gatcatacag cacgagtacg accatctcga tggcatcctg tttgtggatc
     5101 gaatcagccc cgttcggcgg caactcctgc gcaaacagct caaggcgctg gcgaagtcgc
     5161 agggaaagcg atcgtgagaa gcagaacggt tcgttcaggc gctggtgagc gggggacggg
     5221 ctgagatgtg cccgccccct cgctgcttcc cgggtgtgtt gtcgtgaaac cccttcgcat
     5281 cgtgttcatg ggaacacccg aattcagcgt gccgtcgctg gagaaactgg cgtcgggagg
     5341 gcaccacgtt gttgccgtgg tgacgaaacc ggacaaaccg gcgggccgcg gtcgggcaat
     5401 ccagcctcct cctgtcaaac gggcggcgga acggctgggg cttcctgtta ttcaaccgcc
     5461 gtcgctcaga gacacggaca cacactcccg ccttgaagca ctccagccgg acctttttgt
     5521 tgtggtcgca ttccgcgttc tgccggaaga actgctgcga atcccgcgcc tcggggccat
     5581 caacctccac gcgtccctgc ttccgaaata ccggggtgcc gctccgatcc aacgggcgct
     5641 catcaacggt gaacgcgaga ccggtgtaac ggtgttcctg cttgagccga cggtagacac
     5701 ggggcggata cttcgccagc gaccgatcgc catcggtgat gacgaaacct gtggcgagct
     5761 cgcggcgcgg ctgagcgtga caggggcaga agaactcgcg gccgcagtgg acgaccttgc
     5821 gcgcggcgcc gccgtaatga ttccccagaa tgatgccgag gcaaccaccg cgccgaaact
     5881 cacgaaggag gacggcagga tccggtggaa tgaacgcgcg gaagcgatcc gcaaccgggt
     5941 acgtgcgctg aatccttccc ccggcgcgta cgccttgtgg aaggggcagc cgttcggcat
     6001 tcttcgagct gctgtggagg aagctcctgc ccccggtgac gaagcgcccg gcacgatcct
     6061 tcaggcggac gacaggcacg gggtgcgcgt tgcaacaggg gatgggattc tccggctgga
     6121 tatcgtgcag cccgcaggca ggcgccccat ggaaggcgcg gcctgggttc gcggtgctca
     6181 cccgcaaaaa ggggagcgtt tcgaataagg aggggatatg gcgaggggta ccaaggcgga
     6241 acgctggatt cttgccctga cgctcggcgt tgccgttttt ctcttcgttg gcgcgctctt
     6301 gatggtgatt cttgttccgc cggcatagcc gggagaagga cctggacgtt tgagatgaca
     6361 cccagacaga gggttcacgc ggcgctgaac cgccttcctg cggaccgcat tcccattttc
     6421 atgtggtatc atcccgtcac cgcccgccgc ctcgcggcag agcttgacat accggtgagc
     6481 gagctttcca cggtgatggg ggatgatgtg cgccagacgt gggtgggcaa taaccacgcg
     6541 atggagggga ttgtccacga acgtgacggg gaaacgcatc gagatgactg gggcatcgaa
     6601 tgggtgaaat cggggcactt caaccagata cgcagttccc cgctggaaca cgcatcacgc
     6661 gaagaggctc ttgcgtacca atatccttac aactgcgtgg attcgttgat ggcgaacatg
     6721 gtgcccctgc tgcggtacag cgaccggtat ttcatcggtt gtgacgtgtc gccctgcctg
     6781 tttgagatga tctgccgcct gcgagggatg gagcaggcaa ctctcgacct tgcggaagat
     6841 cccgacctcg cacgcacgat gcttgccgaa gccggtgcgt tttcccgcca tcttgcggag
     6901 gaagcatgcc gtcgttttcg gctggactgg ctctggaccg gcgacgacgt gggcggacag
     6961 ctcgcgatga tcatgagtcc gtctctctgg cgtgagctgg tgaaaccgcg cctgaaagag
     7021 atattcgccg ccggcaagac gggaggcatc ccggtggcct accactcgtg cggagcagta
     7081 cgggatatca tacctgatct gatcgaaata ggtctcgatg tactgaaccc catacaatgc
     7141 aactgccccg ggatggaccc cgcttcgctg aaaagggagt ttggccggaa gctcaccttc
     7201 atgggggggg tggatacgca ggggctgctc ccacaggcaa ccgccgatga ggtgttccgg
     7261 gtaacgcgga aacttatcga gatcatgacg gaggacggcg gaggatacat ccttgctgcg
     7321 tcccacacca tccctcccga aactccgagc gagaacatct ttgcgatgta cgacgccgcg
     7381 ggactcagca gagaggccat cgcggacgcc gctgcagacg cccggaaacg aggggcgggc
     7441 ttacgttcct gagagccaat gttgggtatc ggttcgtttt gtagtaccgt gtacgtgtga
     7501 aatccagttg cgggcgcgag gggagtcgcg ccacgatatg caacgacgcg tggagagctt
     7561 gcacacgggt gcctcttcca gaaaggactt ggtgagacgg catggaactt ttgagacgtg
     7621 cgctgttgcc ctgtgtttgc gggatgatct gccttgccgc ggcggggtgc ggccgggaca
     7681 agttcggcaa cgcgctggac aaggtgcttg agcaggacac cgttactacc atcggtgtca
     7741 ttggcgtgtt cggaagcccc ggcaaccacc actacgttga acgagacgct gccctggcag
     7801 cgtacctcgc gaaaatcgca gacggtaagt acaccatcat cacgattgac accgcagttg
     7861 ccaacaaggt gttgagtgtg gtggttcagc cgacgagcaa ctttgcggat cccaaagtgg
     7921 tcgcgaaaat cgggaaagca tttgggttca aaatcgcggt gatcggggag gtgatgccgg
     7981 cagaccccga aaagctggcg gtgcgggcga ttcattcgct ggatgaggag gaaattgctg
     8041 ccggcgaaat accgttcgag gcaccttcca cggcactgcc ggccaatgtg cgcaggcaga
     8101 ctccgccgcc ggtggcgctg aacaccgata tcaaacggtt cttcatcggg catatcaatg
     8161 cggaggaggt cgccgtcacg ctggacgtaa aaaacgtgct gccttcttcc agcaccgagg
     8221 cggagttcct ctacaccatg aatgcgcctg acagccgcca ggacggcaag gggaaactct
     8281 atctgatgga aaacatcgtg gagctggaag gcctcggcgt cggcataatc tacgtcaatg
     8341 ccgacgggaa aatcgtcctg gaatccgcgg acaacaacct gaacagctcg tggctgtttg
     8401 aagaggtggc caaccgatga atcctcgcat cctgttcttc atgctcctgt ttgcgttcgt
     8461 cgtcggatgt ggcggcggag gcgacgaaga ggccgatctt gaatcgtcgg atcccgcgaa
     8521 aagaatcgcc gcgcttcagc ggcaggtaga gcggctgaag cgaaacgacc ggatgaaaga
     8581 cgaatacatc cgcaccgtca cgcggaacat caacgagatt gaatcccgcc tcgattcggt
     8641 tacgcgcggg caggtctccg ttacccgcgt gatgagctcg aatccagaat tgcggatgag
     8701 cagttctcag acgcagaaca tgctcgacga cataaacacg cttcagacgg cgatgaagcg
     8761 gaacaaggct cagattcaaa gcctccgcca gcaggtgagc tcgtcgcggt tcaaaatcgc
     8821 ggagctggaa caactggtaa ccactctgac caaccgggtt gcggagaagg actcgcaaat
     8881 tgccgtgctg gtcagtcgcg tatcagaact ggaaggcacg gtttcgcgac agcaggccac
     8941 gattacccag cagacgcgaa cgctggaaga acaatcccgc ctcagtgccg aacagcaaca
     9001 gacaatcgaa cggcagcagc ggcaactcgg tacgggctat tatacctatg gcacgctgga
     9061 cggtctgaaa agaaacggac ttgttacgga gcgcggaggt ttcctgggca tcggcaaaga
     9121 gaccgtcatc gctccggggt acaacgacga tccgtccaag tttaccgagt ttgacatcca
     9181 gaatgagaac cgtctggtga ttccgcacac gcgctcgcag agcatacaga agattctgcc
     9241 ggaccgggcc agtacatcgt accaacttga acgcgctgcc accggaacga caatccgcat
     9301 catcaacccg gggacgttct ggcgggacaa attcctcgtc atcgtgcttg gcggataact
     9361 cacacgcgct aacgacgggg gcggcgcttc ttggaagagg atggaccgcc cccgtttctt
     9421 tgtgcgcccg aatgtctcac cccgcgaaga ggggcaccat gcataaacct cgccctgtcg
     9481 tattctatta cggacgggaa tcccacgaac cctggcggga tgaactccgc gaagccgcgc
     9541 ccgg
//
LOCUS       DAOSOO010000114         9532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7144_length_9532_cov_6.201752, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000114 DAOSOO010000000
VERSION     DAOSOO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_9532_cov_6.201752"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLT86_09030"
     CDS             complement(<1..488)
                     /locus_tag="PLT86_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOT36795.1"
                     /translation="MTAKTESPIRVQDGTLVVPDVPIIPYVEGDGTGPDIWRAAVRVF
                     DAAVEKAYGGTRRIAWREVLAGEKAFISSGEWLPQATVDDFTHYLVGIKGPLTTPVGG
                     GIRSLNVALRQLLDLYVCLRPVKWFEGVPSPVKHPELVDMVIFRENTEDVYAGLELKE
                     GST"
     gene            843..2972
                     /locus_tag="PLT86_09035"
     CDS             843..2972
                     /locus_tag="PLT86_09035"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT36796.1"
                     /translation="MSFPMPLPSDPDLIHAWTFSRLSAALDSGDAARSSLAASTWRDE
                     RRRRREQIGRVLVPDWEVPDPCVTITGSISRSDKRTGARWKILKLRFQSLQDYWVTAL
                     LYVPETGRGPFPGMIVASGHLLEAKAGAEYHGISEELARQGMVVLAFDPVAQGERVQA
                     WDYVDNKFLVGWGTTEHDYDGQRALLCGWPLAQAFVWDGQRALDVLLSRPEVDPSRVG
                     VCGISGGGTQTTWLLAADDRFTAASPACFITGWREQFAARVGADPEQFPFPANGWGWD
                     QADVLLSFAPKPLLIVAATRDFFPIEGTRSTYQKLKDAYTLIGAAGNVAMFEGDFDHA
                     YHPPLREATVRWFCSVFGISYDGMGAVEAPCNPDDLRVTPTGQLLTSGFPRTLRHWIA
                     QNGSDRSRSRVRGSALYHPDVWQARRRALLSELLGIPSKAPQPSAIPLETNSWGGLTV
                     RRWLLTSEPGVEIPCALVLPPNEPCGLLCFLHEKGAATGWQLAGGPLSEAVDEGWAVL
                     SVDPRGVGAGVCRTQGNFYPDYHGRFGVESHITWTWTMLGRPFFAQRVFDVLQALRWA
                     RNMPDLAGRKSALVGVGSGALWALFASALDGMVEKTLAHAPLFAFGSLLDAHEHSWHV
                     SSLPPGMLTWGDVQHVAALISPRELTLLHPVDAARLPVSRLALSRAFRDARSVFNAQG
                     ASSRLTLVPAEHCGRCERPRYVDWLRD"
     gene            complement(2991..3692)
                     /gene="radC"
                     /locus_tag="PLT86_09040"
     CDS             complement(2991..3692)
                     /gene="radC"
                     /locus_tag="PLT86_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT36797.1"
                     /translation="MPRNNQSATKGGAGPDGRHTPEIDPPMKQLERLGPSGLSDGDLL
                     ALVLDSDGDTIKAAHTIAEEYPLERLVVLPWEEISRVPGITPQSAARLAAAVELAKRG
                     LERGLGGLPKIARPSDVMKVAPELREAKQEHFVCLYLNARTQLIHKTTISVGTLSGTL
                     VHPREVFAPALEHSASAVIVVHNHPSGDPAPSRDDLALTHRLVEAGLIMGIEVIDHII
                     VGRDTWVSLKQTGEL"
     gene            complement(3694..4086)
                     /locus_tag="PLT86_09045"
     CDS             complement(3694..4086)
                     /locus_tag="PLT86_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36798.1"
                     /translation="MVTGRIVRIVDEYTLVADIGLEHGVSVGDEYVVVQPVEPVVDPE
                     TRAELGIWEMVKARLVVVHAQERLSTLMPLPERPAHATVLSERMAWNSQGAPIGSRDV
                     ALPLDRSQMAGRRRTDLIRVGDTVKSVH"
     gene            complement(4123..4896)
                     /locus_tag="PLT86_09050"
     CDS             complement(4123..4896)
                     /locus_tag="PLT86_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT36799.1"
                     /translation="MARARAAAGALAVAAIPGSAPFDAPTVDGFRAAHFTASNGVEMP
                     YRLFVPDSARDGQSYPLILYLHGRAGLGTDNLKQISGGNTYGTRLWTRPDIQSTQPVC
                     VVAPQASGDARWDDRDSGELAPSAEAALELVNALTKQLSLDTNRVYLIGQSRGGFGVW
                     DILSKRPSLFAAAIPVSGGGSPFRARCAVEVPVWAFHGELDPTVPVERTREMVAALRA
                     AGNPVRYTEYAGAGHGIWESVFAEPELPGWLLSQKRRPR"
     gene            complement(4922..6154)
                     /locus_tag="PLT86_09055"
     CDS             complement(4922..6154)
                     /locus_tag="PLT86_09055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT36800.1"
                     /translation="MSELALLGGTPVRTKPFPAWPIWDEKDVEAITRALQSGKWGIAP
                     PDQSEVARFEKEYAAYHGAKHGICVHSGTTAIRIALQALGAGYGDEIIAPAYTFIGTV
                     SPIIDVGAVPVFVDIDPKTYTIDPAAVEAAITPRTVGIVPVHFGGCPCDMDALLAIAK
                     KHGLWIMEDAAQAWGAQWNGTGVGHIGAAGIFSFQSSKNVNAGEGGIVLTDDDDLSER
                     IRSFSNCGRSSQGVWYGHYRIAGNYRMSQILAALLSVQFSRYPEQHRTRETNAAYLAI
                     KLAEIPGFTVLERKPQVTAHAQHLFIWRYDQEAFGGLPRAKFIEAMHKEGIPISPGYS
                     IPLYKQPVFLEKNFDPKHAAQRVDFGSIQCVATEKACAEEALWLTQPILLGETADMDD
                     IVAAALKVRDNAHLLLER"
     gene            6360..7235
                     /locus_tag="PLT86_09060"
     CDS             6360..7235
                     /locus_tag="PLT86_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT36801.1"
                     /translation="MTGFGRGQSSVGGYCVTVEIRSVNSKFIEVSCRIPHALSSLEQH
                     VIEVVQERLGRGKVSVNVSLDNSAGNGTSTVREVIPNLPLAEAYKKAVETVRDHLGMS
                     ADVDLAALVRMDGVLAVRDTQLESETAAALLDGALDAAIEELQTMRCKEGEALAKDFL
                     FRIDRLRELVSLVEARAPERVEEAKRKLTERISALLGSNSVDPQRIAMEVAILSDRAD
                     ITEECVRFRSHLDQFVNLMREDASGRKLNFLLQEMGREVNTTGSKSNDSTIAHLVVEM
                     KEEIERLREQVQNIE"
     gene            7251..7859
                     /gene="gmk"
                     /locus_tag="PLT86_09065"
     CDS             7251..7859
                     /gene="gmk"
                     /locus_tag="PLT86_09065"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT36802.1"
                     /translation="MQSRGFALVISAPSGTGKSTITKAVLEAESRCVQSVSATTRKPR
                     AGEVDGKDYFFVSLDEFERKKRDGELIEWAEFAGNFYGTPRAFIEKAKAHGDIAVLDI
                     DIQGAAQIRRTMPDAVTIFVAPPSLAALEQRLRARKTETEEAIAKRLARVPSELAAAK
                     YYDYLVWNDRVENAVQAILEVIRVESRRISRMDLEGDWLPKM"
     gene            7916..8149
                     /gene="rpoZ"
                     /locus_tag="PLT86_09070"
     CDS             7916..8149
                     /gene="rpoZ"
                     /locus_tag="PLT86_09070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT36803.1"
                     /translation="MLLVDQLTRHGHSVYSIVIAAAKRARKINDWRIQRAKILGEETF
                     GPKPTVQALEEIANGTVKVVREDAGRQQQAISA"
     gene            complement(8222..9496)
                     /gene="aroA"
                     /locus_tag="PLT86_09075"
     CDS             complement(8222..9496)
                     /gene="aroA"
                     /locus_tag="PLT86_09075"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT36804.1"
                     /translation="MKTHARPITPVSSLHATIRPPGSKSLTNRALLCAALAYGESEIS
                     NALVSDDSRYMISALRKLGVDLSVSADGTMIRVRGCSGRFPAKKADLFVGNAGTAMRF
                     LTAALTLGTGVYTVDGDERMRQRPILDLVYGLRALGGDVLACNEPFPPVLIRADGLPG
                     GSCEIDGTTSSQFISAVLMAAPYARRDTVVTVKNGLVSAPYVGMTRHVMRAFGADVEE
                     TEPHVYHVAANSRYFGTTFAVEPDASAASYFFAAAAITGGTIRVTGIGKNSVQGDRAF
                     VRLLEAMGCVVREGADWTEVTGKPLHGISADMADISDTAMTLAVVALFADSPTSIRGI
                     GNVRVKESDRIAAIATEARKLGAQVEEFEDGLRITPAPLRGAMIETYNDHRIAMSFAI
                     AGLRQPGVVISNPECVNKTYPGFFEELTKLGG"
BASE COUNT         1867 a         2841 c         2862 g         1962 t
ORIGIN      
        1 gtggagcctt ctttcagttc caatcctgcg tagacatctt cagtattctc gcggaatatc
       61 accatatcca cgagttccgg atgctttacc gggctcggta ctccttcaaa ccacttcacc
      121 ggccgcaggc acacgtacag gtccagcagt tgccggaggg cgacgttaag gctgcgaatg
      181 ccgcctccga ctggcgttgt caacgggccc ttgatgccca cgaggtaatg cgtgaagtca
      241 tccacggttg cctgtgggag ccattcgccg gaactgatga atgccttttc tccggcaagc
      301 acttccctcc acgcgatacg ccgtgttccc ccgtacgcct tttccacagc ggcatcaaaa
      361 actcggactg ccgcccgcca gatatcaggc ccggtgccgt ccccttccac gtagggaata
      421 attggcacgt ccgggaccac aagcgttccg tcctggactc gtatcggtga ttcggttttt
      481 gcggtcatca tgttcccttt gttgaagaaa tcagtaatac aaacggcgtg ccacaccgtg
      541 tgatgcacgc aaatcactta taaacaatta tttaaattcg tatgcattga aacatgagca
      601 aaattcccca gcaatcaaga ccggcattgg gtaatattac ccaaatggag atgtgatgta
      661 aattacttga acttgcgaat ctcagaggcg ctcgttgctc tccgaagcag tcattctaac
      721 ggggttacga aatgtacgga gacgggttgc ccgtgtctcc ccctccccgt tgcgctggaa
      781 accgctgctg gtgaacttct ccacgtcctg ccagaaacca cccgtgtgcg gaggatgcgg
      841 ccatgtcatt tcccatgcct ctcccttctg accctgatct gatccatgcg tggactttct
      901 cccggctttc ggctgcactg gatagcggcg acgcggcgcg gagctctctt gccgcatcga
      961 cctggcgcga tgaacgcagg cgacggaggg agcagatcgg ccgcgtgctg gtgccggact
     1021 gggaagtgcc cgacccgtgc gtcaccatca ccgggtccat ctcgcgctcg gacaaacgaa
     1081 ccggtgcgcg gtggaagatt ctcaaactgc gcttccagtc gttgcaggat tactgggtca
     1141 ccgccttgct gtacgtgccg gaaacagggc gcggcccgtt cccgggaatg atcgtcgcga
     1201 gcggccacct tctcgaagcc aaagcggggg ctgagtacca cggcatctcc gaggaactcg
     1261 cccgccaggg gatggtggta cttgcgtttg acccggtcgc acagggcgaa cgggtgcagg
     1321 cgtgggacta cgtggacaac aagtttcttg taggctgggg aaccaccgag cacgattacg
     1381 acggccagcg cgcgcttctc tgcgggtggc cgcttgctca ggcgttcgtc tgggatgggc
     1441 aacgggctct cgacgttctg ttgagccggc cggaagtgga cccgtcacgc gtcggagtat
     1501 gcgggatatc aggcggaggc actcagacga cgtggcttct ggcggccgac gatcggttca
     1561 ctgcggcctc gcccgcgtgt ttcatcaccg ggtggcgtga gcaattcgcc gcgcgggtgg
     1621 gcgctgatcc cgagcagttt ccttttcctg cgaacggctg gggatgggat caagcggacg
     1681 ttctgctcag cttcgcgccc aaaccgctgc tcatcgttgc agcgacgcgg gatttctttc
     1741 cgatcgaagg cacgcgatca acgtatcaga aactgaaaga cgcgtacacc ctgattggcg
     1801 cggcgggcaa cgtagcgatg ttcgaagggg atttcgacca cgcgtaccat ccccctctcc
     1861 gcgaggctac ggtgcgttgg ttctgcagcg tgttcggaat ctcgtacgac ggtatgggcg
     1921 cggtcgaggc gccgtgcaat cccgacgacc tgcgcgtgac gccgaccggc cagcttctca
     1981 ccagtggctt cccacgcaca ttacggcact ggatcgcaca gaacgggtcc gatcgctcga
     2041 ggtctcgggt gagaggttct gcgctgtacc acccggacgt ctggcaggct cgtcgccgag
     2101 cgcttttgtc cgaacttctg ggcattccgt ccaaagcccc gcaaccgtcc gccatacctc
     2161 tggaaacgaa ctcctgggga ggacttaccg tacgccggtg gctgctcacg tcggaaccgg
     2221 gggtcgagat tccctgcgcg cttgttctac cgccgaacga gccgtgtggc ctgctgtgct
     2281 ttctccacga aaagggcgcc gccaccgggt ggcaacttgc gggagggcca ctttcagagg
     2341 ccgtagacga gggctgggcc gtgctgtcag ttgatcccag aggagttggt gccggagtct
     2401 gtcgcacgca gggaaatttc taccccgact atcacggccg tttcggggtc gagtcccata
     2461 tcacatggac gtggaccatg ctgggcaggc cttttttcgc acagcgcgtg ttcgatgtcc
     2521 tccaggcctt gaggtgggcg cggaatatgc cggatctggc gggcaggaag agcgcgttgg
     2581 taggagttgg ctctggtgcg ttgtgggccc ttttcgcctc ggccctcgac gggatggttg
     2641 aaaagacgct cgcgcatgcg ccgcttttcg cattcgggtc tcttctggac gcgcacgagc
     2701 attcatggca cgtcagcagc ctgccgccgg gcatgctcac gtggggcgat gtgcagcatg
     2761 tcgctgccct gatctcgcca cgcgaattga cgcttctaca cccggtcgat gccgccaggt
     2821 tgccggtgtc ccgccttgcc ctgtcgagag cattccgcga tgctcgctcg gtgttcaacg
     2881 cgcagggagc ttcatcgcgg ctcacccttg ttccggcgga acactgcgga aggtgtgagc
     2941 ggccgcgcta cgtggactgg ctccgcgatt gaaggctccc ggtacccggt tcacagctcg
     3001 cccgtctgct tgaggctgac ccaggtatca cggccgacga ttatgtggtc aattacctca
     3061 atgcccatga taaggcccgc ctcgacaagg cggtgggtca gcgcaaggtc gtcacgggag
     3121 ggcgccggat cgccggacgg gtgattatgg accacaatca ccgcggaggc ggaatgctcc
     3181 agagcgggcg cgaacacctc ccgcggatgc acgagcgtgc cgctcaatgt gccgacagaa
     3241 atagtggtct tgtgaatcag ttgggttctc gcgttcaggt aaagacagac gaagtgctcc
     3301 tgctttgcct cacggagctc cggggcaacc ttcatcacgt cagaaggccg ggcgattttg
     3361 ggtagtcctc caagcccccg ttccagaccg cgtttcgcaa gctcgaccgc cgctgccaga
     3421 cgcgccgcag actgcggcgt aattccggga acgcgagata tctcctccca gggcagcacc
     3481 acgagccttt ccagcgggta ctcctcggca atcgtatggg cggctttgat cgtgtctccg
     3541 tctgaatcca gaacgagcgc gagcaaatct ccgtcgctca accccgacgg cccgaggcgt
     3601 tccaattgct tcatcggggg atcgatctcc ggggtatgcc ggccgtcggg cccggctccg
     3661 cccttcgtgg cactctggtt gttccgcggc atgtcagtgt accgatttta cggtatcgcc
     3721 aacacgaatg aggtccgtgc gccttctgcc ggccatctgt gaacggtcca gcggcaacgc
     3781 tacgtcacga gaccctattg gcgcgccctg cgagttccac gccatccgct cagacagaac
     3841 agtggcatgc gcaggccgct ccggcagcgg catcaaggtg ctcagcctct cctgtgcgtg
     3901 aactacgaca agccgcgctt tcaccatctc ccagataccg agttctgccc gggtctccgg
     3961 gtcaactaca ggctccacgg gttgaaccac gacgtactca tccccgacgg agactccgtg
     4021 ctccaaccct atgtcggcca caagggtgta ctcgtcaacg atccgaacga ttcggccggt
     4081 caccatgtca ctgactcctt gcgtagcaat tgggttcgcg cctcaacgcg ggcggcgttt
     4141 ttgcgagaga agccacccgg gcagctccgg ttcggcaaac acgctctccc agatgccgtg
     4201 cccggctcct gcatactccg tataccgaac cggatttcca gccgctcgca gcgcggccac
     4261 catttctcgc gtgcgctcga caggtacggt cggatcgagt tcaccgtgga atgcccacac
     4321 ggggacttcg acggcgcatc tggcccgaaa aggcgatccc ccgccagaaa ctggaatcgc
     4381 cgcggcaaac agagatggcc ttttgctgag gatgtcccag actccgaacc ctccgcggct
     4441 ctggccgatc agatacacgc ggttcgtgtc cagcgaaagc tgtttcgtca gcgcgttgac
     4501 gagctcgaga gctgcctctg cactcggtgc aagctcgcct gagtcccggt cgtcccagcg
     4561 agcgtcaccg gaggcctgtg gggcaacaac acataccggc tgcgtactct gaatatcggg
     4621 gcgtgtccac aggcgcgtac cgtacgtgtt tcccccgctg atctgcttga ggttgtcggt
     4681 gcccagtccg gcccgtccgt gcaggtagag gatgagcggg tacgattgcc cgtcgcgtgc
     4741 cgaatcgggg acaaaaagac gatagggcat ctcaacgccg ttcgatgccg tgaagtgcgc
     4801 ggcacggaaa ccatcaaccg tgggtgcgtc aaagggtgcc gaccccggaa ttgcagcgac
     4861 tgcaagggct ccagccgcgg cacgcgcacg cgccatcgtt gcctccattg acaaggcagc
     4921 tctaacgctc cagcagcaga tgcgcattgt ccctgacttt cagagccgcc gcgacaatgt
     4981 catccatatc ggcggtttcg ccgaggagga tgggctgcgt caaccagagc gcctcctcag
     5041 cgcaggcttt ttccgtggca acgcactgga ttgatccgaa gtccacgcgt tgcgcggcgt
     5101 gtttcgggtc gaaattcttc tccaggaata caggctgttt gtagagggga atggaataac
     5161 cgggcgagat gggaatgcct tccttgtgca tcgcctcaat aaacttcgct cgaggcaatc
     5221 ctccgaacgc ctcctggtca tatcgccaga tgaacaggtg ctgtgcgtgc gccgtcacct
     5281 gcggtttgcg ttcgagaacc gtgaaaccgg gtatttccgc gagctttatt gcaagatacg
     5341 cggcgttcgt ttcgcgtgtg cgatgctgct cggggtaacg cgaaaactga acggagagca
     5401 aagccgcgag tatctgcgac atgcggtaat tgcccgctat ccggtaatgc ccgtaccaga
     5461 cgccctggct cgaacgtccg caattactga acgatcgtat ccgttcagac aaatcgtcat
     5521 cgtctgtgag aacgattccg ccctctcccg cattcacatt tttcgacgat tggaagctga
     5581 aaatacccgc agcaccgatg tggccgactc ccgtgccatt ccactgcgcg ccccacgcct
     5641 gtgcggcgtc ttccatgatc cagagcccat gtttttttgc gatcgcgagg agcgcatcca
     5701 tgtcacacgg gcacccgccg aaatggaccg gtacgatccc gaccgtgcgg ggcgtgatcg
     5761 cggcttctac cgcagccggg tcaatcgtgt atgtcttcgg atcgatatcc acgaacacag
     5821 gtaccgcccc tacgtcaatg atcgggctga ccgttccgat gaaggtatac gcgggcgcta
     5881 tgatctcgtc cccgtaacca gcgcccagtg cctgaagggc aatccgaatt gccgtagttc
     5941 cgctgtgcac acagattccg tgctttgcac cgtggtacgc cgcgtactct ttttcaaatc
     6001 gcgcaacttc gctctggtcg ggcggcgcga taccccattt cccgctttgg agcgcccggg
     6061 tgatcgcctc gacgtccttc tcatcccaga ttggccaggc aggaaacggc ttggtgcgaa
     6121 ccggtgttcc accgagaaga gcaagttccg acatcgcgtt gtccccattg tgaattgtgg
     6181 atcatgcaaa acaagactcg ggcaaaggat accgatcctg tgggaaccgg tcaacgggca
     6241 ggacggccgg gaagcaggaa cactcgacaa agtcagagaa attcagtaca ttctaatgct
     6301 ttctctattg cgaaaaactc gaagggtgat tccacggtgg cggttttgtg atacgcagca
     6361 tgacgggttt cggacgaggg cagtcctcgg tgggcgggta ttgcgtcacc gtggagattc
     6421 gctccgtgaa cagcaagttc atcgaagtct cgtgcaggat cccccacgcc ctttcctccc
     6481 tcgaacaaca tgtgatcgaa gttgttcagg agcggctggg aagaggcaaa gtgtcagtca
     6541 acgtttctct cgacaactcc gccggcaacg gcacttccac ggttcgcgag gtaattccaa
     6601 acctgccact ggccgaagcg tacaaaaagg cagtggagac ggtacgggat catctgggta
     6661 tgtctgctga cgtcgacctc gcggcgctcg ttcgtatgga cggcgttctc gccgtccgcg
     6721 acactcagct cgaaagcgaa acggcggctg ctcttcttga tggcgcgctg gatgccgcga
     6781 tcgaggagtt gcagaccatg cggtgcaagg aaggagaagc ccttgcaaag gacttccttt
     6841 tccgcatcga caggctccgc gaactcgtct ctctggttga ggcacgtgca ccggaacgtg
     6901 tagaggaagc gaaacgtaaa ctgacagagc gaatatctgc gcttctcggc tcaaattcgg
     6961 tagatccgca acgcattgcg atggaagtcg cgattctctc tgaccgcgcc gacatcaccg
     7021 aggagtgcgt ccgcttccgg tcgcacctcg accagtttgt caacctgatg cgggaagacg
     7081 cctcggggcg caagcttaat tttcttctgc aggagatggg gcgggaagtc aacactaccg
     7141 ggtccaagtc caatgattcg acgattgcgc acctggtggt ggagatgaaa gaggagatcg
     7201 agcgcctccg ggaacaggta cagaacatcg aatgaacagg gggttgtccc atgcagtcgc
     7261 gcggatttgc tctggtgatt tccgcaccct cgggcacggg gaaatcaacg ataacaaagg
     7321 ccgttctgga ggcggaaagc cgttgcgtac agtctgtatc agccacaacg cgaaagccgc
     7381 gcgccggcga ggttgacggc aaggactatt tcttcgtgtc gctggatgaa ttcgagcgca
     7441 agaagcgtga cggcgaactg atagaatggg cagaattcgc ggggaatttc tacggaacgc
     7501 cccgggcttt catcgaaaag gcgaaggccc acggtgacat cgcggttctc gacattgaca
     7561 ttcagggagc ggctcagatt cggcgaacga tgcctgatgc cgtcacgatt ttcgtggcgc
     7621 cgccctctct cgcggcgctc gaacagcgac ttcgcgcgcg gaaaacagaa acggaagagg
     7681 cgattgccaa acgtctggcg cgtgtccctt ctgaacttgc cgccgcgaag tactacgatt
     7741 acctggtgtg gaacgaccgg gttgagaacg cggttcaggc aattctggag gtgatcagag
     7801 tggaatcccg tcggatttcg cgcatggatc tggaaggtga ttggttgccg aagatgtagt
     7861 aatgcggtga aatgataaat cggtctttgt gaacgaggaa agacggagac gtgaaatgct
     7921 gctggtagat caacttacga gacatggtca ttcagtgtac agcatcgtga ttgctgcggc
     7981 gaaaagagca cgcaaaataa acgattggcg cattcagcgt gcaaagatcc tcggcgaaga
     8041 gacgttcggc ccgaaaccaa ccgtgcaggc cctggaagaa atcgccaatg ggacggtcaa
     8101 ggtggtcagg gaagacgccg gaaggcagca acaggccatc tctgcgtagg tttcttgcgc
     8161 gggcctgatt cctggcaacc ggcagtgcgc aggcgggatt tgtcgcggtc agtgctcgtg
     8221 gtcagccgcc gagtttcgtg agttcctcga agaaacccgg ataggtcttg ttcacgcatt
     8281 ccggattgct gatcacaact ccgggttgac gcaaccctgc gatggcgaaa ctcatcgcta
     8341 tgcggtggtc gttgtacgtt tctatcatcg caccgcgtag cggtgcggga gtgatgcgca
     8401 aaccatcctc gaactcctcc acctgtgccc cgagtttcct tgcttctgtc gcgatggcgg
     8461 cgatgcggtc cgattccttc actctgacat tgccgattcc gcgaatactg gtgggagaat
     8521 ccgcaaagag cgccacgacg gcaagggtca tggcggtatc tgagatgtcc gccatatccg
     8581 ctgatattcc gtgcaggggt tttcctgtaa cctcggtcca gtcggccccc tcgcggacaa
     8641 cacatcccat tgcctcgagc agacgaacaa acgcgcgatc accctgtacg ctgtttttcc
     8701 cgatgcccgt gacgcggatg gtacccccgg ttattgcggc ggcggcaaaa aaatacgacg
     8761 ctgccgacgc atccggttcc acggcgaacg tggtaccgaa gtagcgagaa ttcgcggcaa
     8821 cgtggtagac atgaggctcg gtttcttcga cgtccgcacc gaacgctcgc atgacatggc
     8881 gtgtcatacc gacgtacggc gcggaaacaa ggccgttttt cacggttacc acggtgtcgc
     8941 gtctggcgta cggtgctgcc atcagaacgg cactgataaa ctggctgctg gttgtgccat
     9001 cgatctcaca actgccgccg gggagtccgt cagcgcgtat gagaacgggc gggaatggct
     9061 cgttgcaggc cagcacatcg ccaccgaggg cgcgcaggcc gtagacgagg tcgagaatgg
     9121 gacgttgtct catccgctcg tcgccgtcaa cggtatacac ccccgtgccg agggtcagtg
     9181 ccgccgtgag gaacctcatt gcggttccgg cattcccgac gaacaggtcc gctttctttg
     9241 ccgggaatct gcccgaacac ccgcgcacgc gaatcatcgt tccgtccgcg ctcacgctga
     9301 ggtccacgcc aagtttccgc aatgcgctga tcatgtatct gctgtcgtct gacacgagcg
     9361 cattggaaat ctctgattcg ccgtacgcaa gcgcggcgca caaaagcgcg cgatttgtga
     9421 ggcttttcga gccgggaggg cgaatcgtcg cgtggagcga gcttaccggt gtgatggggc
     9481 gcgcgtgcgt tttcatttct gctctgccct gcgtgttgtg cctccgctga ac
//
LOCUS       DAOSOO010000115         9504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7169_length_9504_cov_5.133030, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000115 DAOSOO010000000
VERSION     DAOSOO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7169_length_9504_cov_5.133030"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLT86_09080"
     CDS             <1..721
                     /locus_tag="PLT86_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36805.1"
                     /translation="AAPADTVAIVFGGKARLSGLWVDDRYVAEYVARHSPRVIGFLSV
                     DPSQPGWETEMREGHEVLGLRGIKLLPMYAGFRPDDRALDGLWSYAEKNRLPVILHSG
                     TTFISQAPLECTLPRWVDNVAIRFPDVRIVLGHLGHPFENEAVAVIRKHPNVYADVSA
                     LFYRPYQLYNSLMLVQEYGVWSKVLFGSDYPFSTVNESVSGLRSLNEMLTGTALPRLN
                     RAEIEAMIHRDSLSLLQLNRP"
     gene            718..1914
                     /locus_tag="PLT86_09085"
     CDS             718..1914
                     /locus_tag="PLT86_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT36806.1"
                     /translation="MNRFALTGARVWTGDGEAFDGHVIVSNGIIERVAQGACGEALPR
                     VDLSGYSLSPGMIDLMVMGGFDRSILRDDPLDIAKEYLRLGVTSCQFCIGTLPWDSMV
                     KVATNISRALSCREYDSARVLGVYCEGPFQQPHLTGASLRDFALEPSPETVERLLADM
                     GNAITMVNVAPGLPGDAEAVTRLVASGKIVSMAHSDAPAERVFRCVEAGTTVLGHAWD
                     NNSGRIGDSGVQQPTIEHVAMTDERIRYLHVIADGVHVHPVMMRLLLRARGSGALCLV
                     TDAVTRAGMPDGPYVWDDGRRFFKSGGVGRTDTGWLCGSATLLPDMFRNYVNFTGLPP
                     HEAIRAVTGNPAASLGMSDEIGHIAAGHIADLVAWDSHLRVRRVWRQGVEISNVSSYG
                     EVEISP"
     gene            1911..2735
                     /locus_tag="PLT86_09090"
     CDS             1911..2735
                     /locus_tag="PLT86_09090"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT36807.1"
                     /translation="MTDRFSLDGKIAVVTGGSRGIGKGIAVGFAEHGADVAIVYRSAQ
                     KEAEAVAERIRGLGRRCWVFRRDLGEVETLSDFVDDIWRTTGRVDVLVNNAGMAYLER
                     FNEITAAHWRRLMAVNLDAVFFMAQRTAEHMIRDGIKGRIINLSSKNGLVAEAGLAHY
                     NASKGGVELVTKSLAIELGEHGITVNCIAPGIIETEIAGEFDIDPAFFSYYKEHIPLE
                     HRFGAVEECVGAAVFLASSAGSYITGQSLVIDGGVLCEQVPRMQFMSTFKNTLNGG"
     assembly_gap    2952..2961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2962..4337)
                     /gene="guaB"
                     /locus_tag="PLT86_09095"
     CDS             complement(<2962..4337)
                     /gene="guaB"
                     /locus_tag="PLT86_09095"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT36808.1"
                     /translation="MRDAIPEGLTFDDVLLVPGESDVAPADVVTTAPFTRQVPVNIPL
                     VSAAMDTVTEARLAIALAREGGIGVIHRNAPVEYQASEVEKVKRSESGMIVDPVTLGP
                     DASIREALTLMSELHISGVPVVDPHGKLVGILTNRDLRFERKLDQPIRNVMTSENLVT
                     APVGVSMEEARATLHRHRIEKLLVVDSEGYLRGLITVKDIEKKIQYPNACTDSLGRLR
                     VAAAIGTGADWEARATALVEKEVDVVVIDTARGHSRRVCSTVQAFKKRFPNVPVVAGN
                     VATREGTKALIDAGVDGVKVGVGPGSICTTRVVSGVGVPQITAVQECAECAQQYGIPI
                     IADGGVKHSGDITKGLAAGASTIMIGSLFAGTDESPGETIIYQGRTFKVYRGMGSLGA
                     MVQGSSERYLQNDVEPEKLVPEGIEGRVPYKGSLSAYVYQLVGGLRSGMGYVGAMTIS
                     ELHEKARF"
     gene            4707..5528
                     /locus_tag="PLT86_09100"
     CDS             4707..5528
                     /locus_tag="PLT86_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT36809.1"
                     /translation="MHTIMDKRLLAPNVYRLEVGAQLIAERRKPGQFIILRVNEESER
                     IPLTIVESDPVAGTIVLVFQVMGEGTRLLASLEMGDEIRDVVGPLGHPTEISHYGTVV
                     CVAGGIGVAPMFPIAKGMADMANTVISIVGARTRDLLILQEEIQSVSSEVLITTDDGT
                     FGRHGFVTDALKERLESGFTPDAVVAIGPLPMMRAVAETTRPYGIKTIVSLNPIMVDG
                     TGMCGGCRVSVGGQMKFACVDGPEFDGHQVNYAELANRQRMYRDKEACKIRSLIP"
     gene            5660..7021
                     /gene="gltA"
                     /locus_tag="PLT86_09105"
                     /pseudo
     CDS             5660..7021
                     /gene="gltA"
                     /locus_tag="PLT86_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
     gene            complement(8240..8599)
                     /locus_tag="PLT86_09110"
     CDS             complement(8240..8599)
                     /locus_tag="PLT86_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36810.1"
                     /translation="MRRSLWIMLAAGAILGSGVFGAINAVQSTRLRIVNGLGNWTIRY
                     VYISPTSSDSWGPDRLGSSEVLRPGESQTWTLDPGSYDLRAKDEDEDTYTRRRVCIPE
                     RMTTEWTVELSDRDTNR"
     gene            complement(8726..>9504)
                     /locus_tag="PLT86_09115"
     CDS             complement(8726..>9504)
                     /locus_tag="PLT86_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36811.1"
                     /translation="LFDFLCEGQVTLSELARFEVVILPHVEYLPLSGRRAVAEYVARG
                     GRVLVTGNTGEFDEHGRPVKMTDLIGRLRSLVWRRGADGTESVDYASTGRIRWLNDLR
                     KWIPGRIWDVHDIEDAMRDEYAEKVLPALRAKAADTPEHDPRLLGMLNDLAGRDLSTT
                     GRGTPPTLRANAWAGTRADKSIVLHLVNYNIPGPGLPPGEVVPCTEVEVRLRVDRHRV
                     KSVCAANPWESPKELPFHQSGNKVRFTVPRIQIYETIHIQ"
BASE COUNT         1966 a         2694 c         2867 g         1967 t
ORIGIN      
        1 tgccgcaccc gcggatactg tggccatagt tttcggtggg aaagccagac tttccgggct
       61 ctgggtggac gaccgctacg tggcggagta cgtcgcccgc cattccccac gggtgatcgg
      121 gttcctttcc gttgacccga gccagccggg ttgggaaacg gagatgagag agggtcacga
      181 agtgctcggc ctgcggggca tcaaactcct gcccatgtat gcgggatttc gtccggacga
      241 ccgggcgctg gacgggctgt ggagttatgc cgagaagaac cgacttcccg tgattctgca
      301 cagcggaacg accttcatct cacaggcgcc cctcgagtgt acattgccac ggtgggtgga
      361 caacgtggcc atccgtttcc cggacgtcag gatcgttctg ggtcatctgg gacatccctt
      421 tgagaacgaa gccgttgcag tcatccgtaa gcaccccaat gtgtacgcgg atgtcagtgc
      481 gctgttttat cgcccttacc agctctacaa cagccttatg ctcgtccagg agtacggcgt
      541 ctggagcaaa gtgctcttcg gaagcgacta cccattctcc acggtgaacg agagtgtttc
      601 cgggttgcgc tctttgaatg aaatgctcac cggcacggca cttccccggt tgaacagggc
      661 agaaatcgag gcgatgatcc accgtgacag cctttctctc ctccaactga accgcccatg
      721 aatagattcg cactgactgg cgcccgcgtc tggaccgggg atggtgaagc ctttgacggg
      781 cacgtaattg tttcgaacgg catcattgaa cgcgttgcgc agggggcgtg cggtgaagcg
      841 cttcccagag tcgatttgtc cgggtactca ctttcgccgg gaatgatcga cctcatggtg
      901 atgggtggtt tcgaccggag catacttcgg gacgatcctc tcgacatcgc gaaggaatat
      961 ctgcgcctcg gggtgacgtc ctgccagttt tgcatcggca cgctcccgtg ggattcgatg
     1021 gtcaaagtcg cgacgaacat ttcgcgagca ttgtcctgcc gcgaatacga ttctgcgcga
     1081 gtactcggag tgtactgcga gggacctttc caacaacccc acctgacggg cgcaagtctg
     1141 cgtgattttg ccctcgaacc ctctccggag actgtcgagc gcttgctggc tgacatgggg
     1201 aacgccatca caatggtgaa cgttgccccc gggctgccgg gcgatgctga ggccgtgacg
     1261 cgtctggttg catcagggaa aatcgtttca atggcgcatt cggatgcccc ggccgagcgg
     1321 gtttttcggt gcgttgaagc cggcacgacc gtgctcggcc atgcatggga caacaactcc
     1381 ggccgcatcg gcgattcggg cgtgcaacag ccaaccatcg agcacgttgc gatgacggat
     1441 gagcgcatcc ggtacctgca cgtcatcgcg gacggagtcc atgtgcaccc ggttatgatg
     1501 cgccttcttc tacgcgcccg tgggtcgggt gcgctgtgtc tggttaccga tgccgttacc
     1561 cgggccggca tgcctgatgg cccctatgtc tgggatgacg gacgccgttt cttcaagtcc
     1621 ggcggcgtgg gccgaactga caccgggtgg ctttgcggca gcgccactct gttgcccgac
     1681 atgttccgga actacgtcaa cttcaccggg cttccgccac acgaggcgat ccgggcggtg
     1741 acgggcaatc cggcggcatc gcttgggatg agtgatgaga ttggacacat cgccgcgggc
     1801 cacattgcag atctcgtagc atgggacagt catttgcgcg tacggcgggt ctggcggcaa
     1861 ggtgttgaga tatcgaacgt atccagttac ggagaggtgg aaatcagccc atgaccgatc
     1921 gtttttcgct ggatgggaaa atcgctgtgg tgaccggcgg cagccgggga atagggaaag
     1981 gtatcgctgt cggatttgcc gaacatggcg ccgacgtcgc gatagtgtac cggtccgcac
     2041 agaaggaggc ggaggcagtc gcggaacgaa tccgggggct ggggcggcga tgctgggtat
     2101 ttcgtcgcga cctcggtgag gtcgaaacgt tgtcagattt cgtggatgat atatggcgta
     2161 caacgggacg cgttgatgtg ctggtaaaca acgcaggaat ggcatacctc gagaggttca
     2221 acgaaatcac tgcagcgcat tggcgtcgcc tgatggctgt caatctggac gccgtgtttt
     2281 tcatggcgca acgcaccgcg gagcacatga ttcgcgacgg catcaaaggc cgcatcatca
     2341 acctgtcatc gaaaaacgga ctggtcgccg aggcaggact ggcacattat aacgcgtcca
     2401 agggtggcgt ggaactggtg accaaatcgc tggccataga gctgggagaa cacggtatca
     2461 ccgtgaattg catcgccccg ggaatcatcg aaacagagat tgcgggcgaa ttcgacatcg
     2521 accccgcgtt cttctcgtac tacaaggaac acatcccgct cgaacaccgg tttggtgccg
     2581 tagaggagtg cgtcggcgcg gcagtgtttc ttgcatcttc tgccgggtcc tacattaccg
     2641 gtcagagtct ggtgatagat gggggcgtgc tgtgtgaaca ggtcccacgg atgcagttca
     2701 tgtccacgtt caagaacacg ttgaacggtg gatgacggaa tcgccggtcg gcaggcaggc
     2761 ccgcgaacac gcaacatgcg agcgctgaac gcagttcccg ccgggaagga tgcggtggca
     2821 gcgatttccc ggaaagggat cacagaaatg gcgtaagagg ctgagtcaac agcctccgat
     2881 ctcaatctgc ccgagcagga tgtttagtgc gtcactccac ccggtaattc ggtgcttcct
     2941 ttgttatcgt cnnnnnnnnn nataaagcgc gccttttcgt gcaactcgga aatcgtcatt
     3001 gcccctacgt atcccatacc ggatcgaaga cccccgacaa gttggtacac gtaggcactc
     3061 aacgagcctt tgtaaggcac tctgccctcg ataccctcag ggacgagctt ttccggctcg
     3121 acatcgttct ggaggtacct ttcgcttgat ccctgaacca tagcgccgag tgagcccatc
     3181 cctctgtaaa ccttgaacgt gcgcccctgg taaatgatcg tttccccggg gctttcgtcc
     3241 gttccggcaa aaagagaccc gatcattatc gtgcttgcac cggcggcgag gcctttggta
     3301 atgtctccgg aatgcttgac tcctccatcc gcgataatcg ggatgccgta ttgctgcgca
     3361 cactccgcgc attcctgtac cgcagtgatc tggggtactc cgacgccgga aacgactcgc
     3421 gtcgtgcaga ttgaccctgg ccccacacca actttgacgc cgtccacccc ggcatcaatg
     3481 agagctttcg tgccttctct ggtcgcgaca tttcccgcca ccacaggcac attcgggaac
     3541 cgcttcttga acgcctgaac ggtggaacac acgcgcctgg aatggccacg cgcggtgtca
     3601 atcaccacca catccacctc tttttccacc agcgccgtgg cccgcgcctc ccagtccgcc
     3661 cccgtaccta ttgccgccgc aacacgcagt ctgccgagac tatcggtgca cgcgttcggg
     3721 tactggatct tcttctcgat atccttgacg gtgatgagac cccggaggta cccctcgctg
     3781 tcgacaacaa gaagtttttc tatgcgatgg cggtgcaggg tagctcgggc ctcctccatg
     3841 gatactccga ccggagccgt gaccagattc tcgctcgtca tcacgttcct gattggctga
     3901 tcgagtttcc tttcgaaccg caggtcccga ttcgtgagta tccctacgag cttgccgtgg
     3961 gggtcaacca cgggaacacc cgatatgtgc agttcggaca tcagagtcaa tgcttcccga
     4021 atggaagcgt cgggtcccag ggtgacggga tcgacgatca ttccgctttc cgagcgcttc
     4081 accttttcga cttcggaggc ctggtattcg acgggagcat tgcggtggat gacgcctatt
     4141 cctccctccc gggcaagcgc gattgcgagg cgagcttccg tgacagtatc catcgcggcg
     4201 ctgacaaggg gaatgttcac gggaacctgg cgcgtgaacg gcgcggtcgt cacaacatct
     4261 gctggagcaa cgtcagattc accgggaacg agcaggacat cgtcgaatgt caggccttct
     4321 gggatcgcat cgcgcatggt tccatttctc cgtgcggcgt tggggcgtcg catcccgggg
     4381 tggggtgttc tgggtgaatt ggtaaaatat gccgccattt cgtttgcgaa gtcaagacat
     4441 cgaaacaccg gcgtctgaga cgggccatca cgaaaactcc aagattcaca aacaggcgcg
     4501 cgttggaaga caggtgtgtc acgcggtata ttgaaaaacg ttcaatggac cacacacgca
     4561 acggctgcag gaacctgctc actgaaatac ctcgcgccgc cccaccgaat tgatcggatt
     4621 tccgcccggt gagattgtac gttgtcctcg gacaccgttc gtgaaccggg agcagtgacg
     4681 gcatactgcg tctgggaggt gaactcatgc acaccatcat ggacaagcgt ttgcttgcgc
     4741 ccaatgtgta caggctcgaa gtcggcgcac agttgatcgc cgaaagaagg aaaccggggc
     4801 agtttatcat tcttcgggtg aacgaggagt cggagcgcat cccgcttaca atcgtggaaa
     4861 gcgaccctgt ggcgggaaca atcgttctcg tgttccaggt gatgggcgag ggtacgcggt
     4921 tgctggcttc tctcgaaatg ggtgatgaga ttcgggatgt tgtcggacct ttgggacatc
     4981 ccacggaaat cagccactat ggcacggtgg tctgcgtcgc cggaggaatc ggtgtggccc
     5041 cgatgtttcc gattgccaaa gggatggccg acatggcgaa tacagtgatc agtatcgtag
     5101 gggcgagaac gcgtgacctt ttgatacttc aggaggaaat tcaatcggtc tcctcggaag
     5161 tgttgattac taccgacgac gggaccttcg gccggcatgg gtttgtcact gatgcattga
     5221 aggaacgcct cgagtcaggt ttcacaccgg acgctgtcgt ggcgataggc cctcttccga
     5281 tgatgcgggc agtagcggag acgactcgcc cttacggcat caagaccatc gtcagcctga
     5341 acccgatcat ggttgacggc accggaatgt gtggcggttg ccgcgtttct gtgggcgggc
     5401 agatgaagtt tgcctgtgta gacggaccgg aattcgacgg gcatcaggtg aactacgcgg
     5461 aactggcgaa tcgtcaacgg atgtaccgcg acaaagaggc gtgcaagatt cgaagtctga
     5521 taccctgatc tcccaacccc acgtgtgcaa cactgacgag gaactgctcg gaaaaggctg
     5581 ccaaatgaac ccttcagaaa atgctgttga aaggaaccct gagcgacgca aacctcaacc
     5641 tgcgcggccg cgacaggtaa tgcctgaacg tccgccgttg gagaggaacc gggactttgc
     5701 cgaggtcgct accgggttta ccgccgaaat ggcaatcgcg gaatcagaac gatgcctcca
     5761 gtgcaaaaaa ccgctctgcg ttcaggggtg cccggtaggc attgacatcc cggcattcct
     5821 ccgtttcatc gctgacgggg atttcgcggg ttcgataaac aggctgaagg aggacacgaa
     5881 tctccccgcg atctgcgggc gagtgtgccc tcaggagact cagtgcgaag aaaagtgcat
     5941 cgttggcaaa cggaaccaac cgatcgcaat aggtgcgctg gaacggttcg tcgctgattg
     6001 ggaaagggag aacggcgccg tcaaaacacc cgtagttggt gcaccgacag gaaaaagggt
     6061 cgcggtggta ggttcgggac ctgctgggtt gacgacggca ggcgaactcg cacgtcgggg
     6121 acacgccgtg actgtttttg aagcgttgca cgagccgggt ggagtgctcg tctacggcat
     6181 cccggagttc cgtctcccga aagaaatcgt ccggcgcgag gtggacgggt tgcgggcaat
     6241 gggagtggag atccggctca acaccatcgt cggcagatcc cttaccattg acgacatgtt
     6301 cgcgcagggg ttcgatgcgg ttttcgtggg ctccggcgca gggctgccac atttcctcgg
     6361 aatccccggt gagaacctca acggcgtata cagcgccaac gagttcctca cccgcatgaa
     6421 cctgatgaaa gcgttccggt tcccggacta ccacaccccg gttcacgtcg gcaaaaagat
     6481 cgcggtattc ggtggtggca atactgcgat ggatgccgcc agaaccgcac tcagaatggg
     6541 tcccgaatcc gtgaaactca tctaccgccg ttcagaagcg gaaatgcctg cgcgtgcgga
     6601 agagattcac cacggacgag acgagggagt ggagtttgtt acgctgactg cgccggtgga
     6661 attccttgac gacgggaagg ggtgggttgc cggtgtcaaa atcatcaaga tggaactggg
     6721 cgaacccgac gcgagcggga gacggcgtcc ggttccgatt gaaggaagtg aagagataat
     6781 cgagatagac accgccgtga tcgccatagg taacagcccc aatcaactga ttccgcagac
     6841 aacaaaaggg ctcgagacca cacggcacgg aacattaatt gcgaatgaag ccaccggcga
     6901 gacgacgaaa accggagtat tcgcaggcgg tgatgtcgtg acgggtgccg ctaccgtgat
     6961 tcaggccatg ggcgcgggaa aggccgcggc ggccgccata gacgatttcc tgatgaaacg
     7021 cgcgaacgcg gaagaactca ggcagtaccg cgagaagtta tcggggttct tccttgtaga
     7081 ttcacctcga cgaacgccgg aagaacgtct tgcgctcctg cgctctctcg gcgtgcccga
     7141 accggaatct tttctcgcca agcggttgcc gcaatgggat gcacgcgtag acgaactgct
     7201 tgatccttcg tcacttgaca tgatgccggt ccacgtttcg catgcacagg cgcggtacgt
     7261 gcgaggtgcc atccaaagcc ttccggaaga cgtgaaggcg cgcatttttt ccgcaaaact
     7321 gggcccgaca ggagttggcg aggctattag gtcgctttac ctgcgcgaaa aaggcatttc
     7381 gctaccggag gatttcgcag ttgcttcggt aactctgctc gacgatctgc gtcagtccgt
     7441 tcgggtggtg gttcaggacg gagggaggag tctcaccgtt gacgtcgtgc gcatgaatgc
     7501 ggacgcagag attgtgtatt ccgcagttgc ggaggaactc ggaatcgctc actgccgggc
     7561 cgagatcgtt cccacgccca aaggtgacaa agctgcggtt tcagtggtac cgagtggtgt
     7621 gaggctttcg gatgagggcg cgctaagcga cgagtatttt cgcgagcagt ggcctttcct
     7681 ggtagataag gttgcggaac aggaggcact tgccgatttc cttggaacgg aaaccggtcc
     7741 cgattcctac acaatcacga ctgaaccggt ttcagtcgtc gcagacaacc ccctcgaaat
     7801 gttccactac ctgccagttt ccaacagagc gaatgagccg gtgtttaagt tcctgtttgc
     7861 gaaggcgttg cctgacgcgc cggtggcaag ggcaaaaacc gcgcgcgact tcgtcgtgcg
     7921 gtacgagacc gcgtacggcg ctgcagccgc tcgtatcaaa gagcactgga acggcataga
     7981 agctgcactc gcaacgagaa aggatgccgt ggaaagctgc tccggcgtgg agctgaaaga
     8041 ggcgctgggg attttgaaag acacgttcag atggaacccg gtttcgcggc tcacgaggat
     8101 gtacgagacg tgcatccccg tgcagtggag tgctatcggg aaagacgtct gggtgtggag
     8161 gcgacagaaa caagcctgag tacggtaccc ggcgagggca tctcgaaaag gatgccctcg
     8221 cattatttcg tatcaggtgt taccgattgg tgtctctgtc ggaaagttcc acggtccatt
     8281 cggttgtcat gcgttccggg atgcacaccc gtctgcgtgt gtacgtgtct tcgtcctcgt
     8341 cttttgcccg cagatcatag ctccccgggt ccagtgtcca ggtttgtgat tcgcccggac
     8401 gcagcacctc gctgcttccc aaacggtccg gtccccaact gtcgctggac gtgggcgaga
     8461 tgtacacgta tcggatagtc cagtttccga gcccgttcac gattcggagc cgtgtggact
     8521 ggacggcgtt gatcgccccg aacactccag acccgagaat cgcccctgca gcgagcatta
     8581 tccacagcga acgccgcatg ctagcctccc tttccctggt gtggcccccc tcgtcaacac
     8641 aactgcagcg ccggggtcag ggtgtgcagt cgatgcaaaa cctgcatgga actcagacag
     8701 gccttcgtgc aaaatataca cttgttcact gaatgtgaat agtctcgtat atctgtatgc
     8761 gaggcacggt gaatcgtact ttgttgccac tctgatggaa cggcagctct ttcggggact
     8821 cccacggatt tgccgcgcac acggacttca cgcggtgcct gtcaacgcgc aatcgcactt
     8881 ccacctccgt acacgggaca acttcacccg gtgggaggcc ggggccgggg atgttataat
     8941 tgaccaggtg caacacgatt gacttgtcgg ctcgtgttcc cgcccacgcg ttcgcgcgca
     9001 gcgttggggg tgtcccccgc cccgttgttg acaaatctcg tcccgccaga tcgttcagca
     9061 tgcccagtaa cctcgggtcg tgctccggcg tgtccgcggc cttcgcacgg agcgccggaa
     9121 gaaccttttc cgcgtattcg tcgcgcatcg catcttcgat gtcgtgaacg tcccagatgc
     9181 gtccgggtat ccacttcctc aggtcgttca accaacgaat tcttcccgtg gacgcatagt
     9241 ccacagactc ggtaccgtcg gcaccgcgac gccatacaag cgacctcagg cggccgatca
     9301 ggtccgtcat tttcacagga cggccatgtt catcgaactc acccgtattt cccgtgacga
     9361 gcacccgccc gccccgcgcg acgtattctg caaccgccct cctgccagag agcggcaggt
     9421 actccacgtg cggcagaatt acgacttcga agcgcgcaag ttctgaaagc gtcacctggc
     9481 cttcgcacag gaaatcgaac agga
//
LOCUS       DAOSOO010000116         9488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7180_length_9488_cov_6.012721, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000116 DAOSOO010000000
VERSION     DAOSOO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_9488_cov_6.012721"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2444)
                     /gene="infB"
                     /locus_tag="PLT86_09120"
     CDS             complement(<1..2444)
                     /gene="infB"
                     /locus_tag="PLT86_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT36812.1"
                     /translation="MRKHKRVYQVAKELHVSSQVLMSCCASLGIEVKTHMDPLEDEDL
                     DRLTKVFEKYPDGIIPAEPASDAPPEGAEAVSVVAAEPEPVAPATPPAAPMPPPSRVF
                     MPPVDTAPIRPQLSTPAPAKPVEPAQEKPEPEKPAVQKERPALRPLSRPGVPDQQRPQ
                     RSFPPPAGSSALPPLRRPGASEAPRRPEAPARPERPALKPLRPRRSEGAEDISRADRL
                     RKMELENEDPEFAEWAKAKTIPLPGSGTIEPVADTGDVADSGLGDAAGVRRRRGKRRK
                     KPTASQSEIQASVARTLSQMGRGRVKRTYERHSQTSQTEEADDSNVLRVSEFVSVSEL
                     ADLMGVKATEVIATCLKLGLMVSVNQRLEMDTIQIIADEFGFEVQQMQELEIEEPEEE
                     EDSPDSLVPRPPVVTVMGHVDHGKTSLLDYIRRTNVIAGESGGITQHIGAYEVETPQG
                     RVTFLDTPGHEAFTAMRSRGAQVTDIVVLVVAADDNVMPQTIEAINHARAAQVPIIVA
                     INKIDLPTANPARIKQQLTQYGVILEDFGGQVQSVEISAKFGQGVDTLLERLALETEL
                     MELKANPNRPARGVVIEARLDKGRGPVATVLVQNGTLRVGDAFLAGMQAGRVRALLDE
                     RGNPIKDAGPSRPVQVLGFDDVPMAGDKFAVYESEREAREIAQYRQQIKREQDYSRIK
                     KVSLTDLKRRISEGELRLLPVIVKGDVDGSVEALSDELARLSNAEVAIQVIHRGVGAI
                     SESDVLLASASNAIIIGFHVRPDARARELAAQEQVEIRLYQVIYEAIEEVKASLAGLL
                     DAKITEQVTGTAEIRQV"
     gene            complement(2503..3900)
                     /gene="nusA"
                     /locus_tag="PLT86_09125"
     CDS             complement(2503..3900)
                     /gene="nusA"
                     /locus_tag="PLT86_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT36813.1"
                     /translation="MNRDDVLEALRQVAREKNVTMDLVIDTLEEGLLSAAKKKFGLSD
                     NIDVEVDQNTGEIVIWAHKAVVEKVEDPAIEISLADAKKIDPEAAIGEGVAQPIPFTA
                     FGRMAIQSAKQMLIQKVREAERQRVYETFREAISDIRIGTAQQVLRGDVIVDLGRAEG
                     ILPVSEQIPRDRYRQKDTVRAYVKDVLKDTKGPQVILSRTHPGFLERLFQMEVPEVYD
                     GTVEVIAVARDPGSRAKVAVASRDDRVDPVGACIGQKGMRIQQVVRELNNERVDIIEW
                     SPDPVVFFQRSLAPAEIQRVEVDQERQTMIAIVDEDQLSIAIGRGGQNARLAAQLTGW
                     RVEIMTEEQYAKKKERDEFLRVPVSELPGSTPAVAKALTEAGIGSALELSERSAVEIA
                     DIAGISAERAAQLLDEATDLLEAKRQEYAERLQIPLEETGVDLATFVAEPSYGLAATD
                     EPGIAETSPEAPGLT"
     gene            complement(3897..4427)
                     /locus_tag="PLT86_09130"
     CDS             complement(3897..4427)
                     /locus_tag="PLT86_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36814.1"
                     /translation="MQQEERDSVQNVATAIAGPMGVEVLEIKWLGSGRTRSVRLVVDQ
                     EGGVGADTCARLSREFDLEWEARTATPRDFALEITSPGPDRPLRTVKDFSRVVNRWVQ
                     IERQSDAGTDTLTGKVVSCSEDALVLSESGIFPDAGDLSGCVAVPFAEIRNAKILFVI
                     GNTTPRGKMARRTTRR"
     gene            complement(4529..5053)
                     /locus_tag="PLT86_09135"
     CDS             complement(4529..5053)
                     /locus_tag="PLT86_09135"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOT36815.1"
                     /translation="MTDLPGLAHARAITGRACAILVALAASGCGVYSVEGGLPSHVRT
                     VGVEFFENSTTEAGVEERLSRAISDQLVTRSQVRYAPFSGADAIVRGSVTRVSEEPLT
                     FSGTQVSRYRVTVLVDASVWDRARRRNLWEREGIQGQGQYDATAGGAGRDAAYASAIQ
                     EAARQVVDGFLSGW"
     gene            complement(5050..6075)
                     /gene="mtnA"
                     /locus_tag="PLT86_09140"
     CDS             complement(5050..6075)
                     /gene="mtnA"
                     /locus_tag="PLT86_09140"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT36816.1"
                     /translation="MKIKTLEWVGDGLRLLDQTLLPNDERYIVCTDVEVLAHAIETLQ
                     VRGAPAIGVAAAYGVVIAAMNAGPSHRREAVAAAIARLRRTRPTAVNLFWALDRMRSV
                     ADVLADGDLVTGLLKEAQAIEVDDTRRCEAMGRHGAALLPDEATVFTHCNAGGLATSG
                     YGTALGVFYAATEMGKKLRVFADETRPLLQGARLTAWELSRAGIDVTVICDNMAAAVM
                     RQTKVDGVFVGADRIAANGDAANKIGTYGLAIIARHHNVPFYVVAPMSTVDISIPDGS
                     GIPIELRNENEVRRGFGPNTVPESAQVFNPAFDVTPADLLSGIVTEFGIARPPYSATL
                     KTWLEAV"
     gene            complement(6072..7010)
                     /locus_tag="PLT86_09145"
     CDS             complement(6072..7010)
                     /locus_tag="PLT86_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT36817.1"
                     /translation="MPDSLLIVGSVAYDNVETPGGRVENALGGSALFGAAAASLFAPV
                     HLVGIVGTDFDRAGIAFLEERGVDLAGLETAEGKTFRWTGVYEEDMNNRRTLATELNV
                     FADFAPRIPEGYRGDKVVFLGNIAPRLQLHVLDQMAGPDWVALDTMDMWIRDAADDLA
                     RVLQRVHMVVVNDSEARALTGKTNLTVAARSILDMGPRAVVVKKGEHGVLLAAEDEFS
                     VLPALPLKTVVDPTGAGDSFAGGMLGYIAATGDFTFSGLRRALAYGTAAASLAVEDYS
                     FNRLRNATREVLDERYRWLRSISFLPDLHGQERHNA"
     gene            complement(7129..8301)
                     /locus_tag="PLT86_09150"
     CDS             complement(7129..8301)
                     /locus_tag="PLT86_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36818.1"
                     /translation="MSEGQKCLDLGIVGLGECGGNLAVEFTRLGYKAVAVNTSHTDLR
                     ALSLDPRKRIYIGYEGRDGAGQDMALGQRSLEASSDIIVEAVGTQLEGCRHLLLCAGL
                     GGGTGSNIAVLANILARLDIPISALASLPKDHESSIAKINAVNAINLFRNSDVASIIL
                     VDNEKILRQYRSESLTAFYSSANQAAVERLHEMNTLSTNELYMPIRGFDGEDFRRVFA
                     SRGVLIYGAADLTDDDLLIRDRLANGLRSIWDSSGLLASGFDYKDATMAGIILVAPQD
                     LLDRAPADIFESLIRQIRELTDTSGIYAGLFQGPPDQMPRLYTMLGGLPFPNRLRTVL
                     GQAKEEGPALGVNVARPIEELDLGELSGLDLFSAGYIGPVPAGVGGQPSPTERRPR"
     gene            complement(8316..8774)
                     /gene="rsfS"
                     /locus_tag="PLT86_09155"
     CDS             complement(8316..8774)
                     /gene="rsfS"
                     /locus_tag="PLT86_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT36819.1"
                     /translation="MIQKKARSRRKPARDAAPVPETVGVLVEAVRSKKASNVVVMDLR
                     GLTDVADFFVLCTADVDVHARAILDAVREALVPFGRNPWHVEGEESLNWVLVDFVDTV
                     VHIFREDARQFYGLEEMWADAPRVPFPDVPPRVGETLHAGSLPPADNPRS"
     gene            complement(8818..9321)
                     /locus_tag="PLT86_09160"
     CDS             complement(8818..9321)
                     /locus_tag="PLT86_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36820.1"
                     /translation="MKTVAILVAGVLALAAQTATASGPFVTNTGERGPTIAGAPTADL
                     VSLMRRQGLFDSSKLTTWRSYSFGIASSGGTTTSAGLLLQHIQYQIATPLTLRMDVGL
                     LHNPLGVAGFRNGPQQASIVMPALDLVYRPTENMVLSVHYSQMPSNRYGYGYGYNAWW
                     NDPSTGW"
BASE COUNT         1830 a         3085 c         2659 g         1914 t
ORIGIN      
        1 acctgcctga tctctgcggt cccggtaacc tgctcggtga tcttggcatc cagaaggccc
       61 gcgagggagg ctttgacctc ttcaattgcc tcgtagatga cctgataaag acgtatttcc
      121 acctgctcct gagcggcaag ttctctggcg cgcgcatccg ggcgaacgtg gaacccgatg
      181 atgatcgcgt tcgaggcgga agcaagcagc acgtcgcttt cactgatcgc gccgacgccg
      241 cggtggatta cctgaatcgc cacttcagcg ttggacagcc gtgccagttc gtcactcagc
      301 gcttccacgg aaccgtccac gtcgcctttg acgatgaccg gcaggagccg cagctcgcct
      361 tcgctgatcc gtcgcttgag gtcagtgagt gagactttct tgatccggct gtagtcctgc
      421 tcccgcttga tctgctgccg gtactgtgcg atttcacgag cttcgcgttc gctctcgtag
      481 accgcaaact tgtctcccgc catgggaacg tcatcaaagc ccagaacctg caccggcctg
      541 ctgggacccg catccttgat cgggtttccg cgctcgtcga gcagtgcgcg cacccggcct
      601 gcctgcattc ctgcaaggaa ggcatctcct acgcgcaacg tcccattctg aacgagtacg
      661 gtggcaacag gcccacgtcc cttgtcaagc cgcgcttcga tcacaacacc gcgggcaggc
      721 ctgttgggat tcgctttcaa ttccatgagt tcggtttcga gcgcgagacg ttccagcagt
      781 gtatcaaccc cttgcccgaa ctttgcggaa atctccaccg actggacctg cccaccgaag
      841 tcctccagaa tgacaccgta ctgcgtgagc tgctgtttga tgcgcgccgg gttggccgtc
      901 ggaaggtcga tcttgttgat ggctacaatg atgggaacct gcgccgcacg tgcgtggttg
      961 attgcctcga ttgtctgagg catcacattg tcatccgcgg cgacaacaag aaccacgata
     1021 tcagtcacct gtgcaccgcg tgagcgcatg gcggtgaacg cttcatgacc cggtgtgtca
     1081 aggaatgtca ctcgtccttg gggcgtttcg acttcgtacg cgccgatatg ctgggtgatc
     1141 cccccggact cgccggcgat gacgttcgtc cggcggatgt agtccagaag cgaagtcttt
     1201 ccgtggtcca catggcccat aaccgtgacg actggtggcc gtggaacaag ggaatccggg
     1261 ctgtcttctt cctcttccgg ttcctcgatc tcaagttcct gcatctgctg gacttcaaat
     1321 ccaaactcgt ccgcgataat ctggatggtg tccatttcca gacgctggtt gaccgacacc
     1381 atgagcccga gcttgaggca cgtcgcgatg acttccgtgg ccttcacacc catgaggtct
     1441 gcaagctcgc tcaccgaaac gaattcgctc acccggagca cattcgaatc gtcagcctct
     1501 tcggtctggc tcgtctgcga gtgacgctcg tacgtccgct tcactcgacc gcgtcccatc
     1561 tgcgaaagcg tgcgggctac gcttgcctgt atttctgatt gagaggcagt cggcttcttt
     1621 cttcgtttcc cgcgtctgcg ccgcacacct gccgcgtcgc ccaggccgga atcggcgaca
     1681 tcgcctgtat ccgcaaccgg ctcgatcgtg ccgctacccg ggagtggaat cgtcttggct
     1741 ttcgcccatt cagcgaattc cggatcctcg ttttccagtt ccattttgcg gaggcgatcg
     1801 gcacgcgaga tatcctccgc cccttccgat ctgcggggtc ggagcggctt cagcgcaggg
     1861 cgctccggcc gcgcgggtgc ttccgggcgc ctcggcgctt cagaagcgcc cggcctccga
     1921 agaggcggga gagccgatga acctgccggc ggcggaaacg atcgttgcgg acgctgctga
     1981 tcgggaacgc caggacggga caacggcctc aacgcaggac gttctttctg gacggccggt
     2041 ttttctggct ccggtttttc ctgcgcaggc tcaacgggtt ttgccggggc cggtgtgctc
     2101 aactgcggtc ggatcggagc cgtatcgacg ggaggcatga acacgcgcga aggcggcggc
     2161 atcggagcgg cgggaggagt tgccggcgca acgggttccg gctcggccgc aacgaccgaa
     2221 acggcttctg ccccctcagg tggagcgtcg ctggcgggtt ccgcagggat aatgccgtcg
     2281 ggatactttt caaatacctt ggtcagacga tcgagatcct cgtcctccaa cgggtccatg
     2341 tgcgttttca cctctatacc gagcgaggcg caacagctca tcagaacctg tgaactgacg
     2401 tggagctctt tcgctacctg ataaactcgt ttgtgctttc gcaacgcgta cgttcctccc
     2461 gaaattcggc atccttacac aggaagggca acattagtcc tactacgtca gacccggggc
     2521 ctccgggctt gtctccgcga ttcccggctc atccgttgct gccaggccgt aggagggctc
     2581 cgcgacgaac gttgccagat ccacgcccgt ttcttccagc ggaatctgaa gccgttccgc
     2641 atactcctgc cgtttggctt ccagcaagtc ggttgcttcg tccagcaact gggccgcccg
     2701 ttccgccgaa attccggcaa tatccgcgat ctccaccgcg gaacgttccg aaagctccag
     2761 agcggagcct ataccggctt cggtgagagc cttggcaact gccggtgtcg aaccgggaag
     2821 ctccgagacg ggaacacgaa ggaattcgtc gcgctccttc ttcttcgcgt actgctcttc
     2881 cgtcatgatc tcgacgcgcc aaccggtgag ttgcgccgcg agacgcgcgt tttggccgcc
     2941 gcgaccgatc gcgatggaga gctgatcctc atcaacgatc gcgatcatcg tctggcgctc
     3001 ctggtcaacc tcgacgcgct gaatctccgc cggtgccaga gagcgctgga aaaatacaac
     3061 aggatccgga gaccattcga taatgtcaac acgctcgttg ttcagttcgc ggaccacctg
     3121 ctggatccgc atgcctttct ggcctatgca ggcgcccacc ggatccacac ggtcatcgcg
     3181 gctggcaact gccaccttgg cgcggcttcc gggatcgcga gcaaccgcga tcacttcgac
     3241 agtgccgtcg tagacttcgg ggacttccat ctggaacaac cgttcgagga agccgggatg
     3301 ggtgcgggaa aggatcacct ggggtccctt ggtgtccttg agaacgtcct tcacgtaggc
     3361 acggaccgtg tccttctggc ggtagcgatc gcggggtatc tgctcggata cgggcagaat
     3421 tccttccgcg cggcccagat ctacgatcac gtcgccacga agcacctgct gtgcagttcc
     3481 tatccggata tcactgatag cttcgcggaa cgtctcgtac acccgttgtc gttctgcttc
     3541 gcgcactttc tggatgagca tctgcttcgc ggactggata gccatcctcc cgaacgcggt
     3601 gaagggaata ggctgtgcca ctccttcgcc aatcgcggct tccggatcta tcttcttcgc
     3661 gtcagccagg gaaatctcga tggctggatc ctcgaccttt tccaccaccg ccttgtgcgc
     3721 ccagatgact atttcacccg tattctgatc cacttccacg tcgatattgt cggaaagacc
     3781 aaatttcttc ttcgccgccg aaagcagccc ctcctccaac gtatcgatga cgagatccat
     3841 cgtaacgttc ttttcgcggg ctacctggcg tagtgcttcg agcacatcgt cacgattcat
     3901 ctgcgtgttg ttctccgtgc cattttgcct ctcggagtcg tgtttccaat gacaaaaaga
     3961 atttttgcgt tgcgtatttc cgcgaacgga accgcaacgc agcctgaaag gtcccccgca
     4021 tcgggaaaga tcccgctttc ggacaggacc agcgcatctt cagaacagga caccactttc
     4081 cctgtcagcg tatcggttcc cgcatcggac tggcgttcga tctgaaccca cctgttgacg
     4141 acccgcgaga agtccttcac cgtacgaagg ggacgatcgg gacccgggga agtgatttcg
     4201 agggcaaaat cccgcggcgt ggccgtccgc gcttcccact cgaggtcaaa ctcgcggctc
     4261 aggcgggcgc aggtgtctgc ccctactccg ccttcctgat ccacaaccag tctgaccgac
     4321 cgtgtccttc cggaaccgag ccacttgatt tccagaactt ccacacccat cgggccggca
     4381 atcgcggtcg caacgttctg gacggaatct ctttcctctt gctgcacaaa gcgccccaat
     4441 atgaaaagag tgagcgcgcg cccactcata aatcatatga aggtacgcat tttcgcggtc
     4501 gaacgcaacg acgcgtgcgt agggagtcct accagccgct gagaaacccg tcgaccacct
     4561 ggcgggccgc ttcctgaatc gccgaggcat aggccgcatc tctccccgca ccccccgccg
     4621 tcgcgtcgta ctgaccctgc ccctggattc cttcccgttc ccacagattt ctgcgccggg
     4681 cgcgatccca gaccgaagcg tccaccagaa ccgtcacgcg gtaacggctg acctgcgtcc
     4741 cggaaaaggt gagcggctcc tcgcttacgc gcgtaacact tccccgcacg attgcgtcag
     4801 caccggaaaa cggagcgtaa cgaacctgcg acctggtaac cagttgatcc gagatggctc
     4861 gcgaaaggcg ctcctctacg cccgcttccg tcgtcgagtt ttcgaaaaac tcgacgccaa
     4921 ccgttcttac gtgcgagggc aatccgccct ccacggagta cacaccacat cccgaggcgg
     4981 ccagcgccac caatatggcg catgctctgc ctgtgattgc acgcgcgtgc gcaagtccgg
     5041 gcaaatccgt cacacagcct ccaaccatgt tttgagcgtt gcggagtagg gaggacgggc
     5101 aataccgaac tccgtgacaa tcccggagag cagatcggca ggagtaacgt cgaacgcagg
     5161 gttgaacacc tgggccgatt caggaacggt attcggtccg aacccgcgtc tgacctcgtt
     5221 ctcattccgc aactcgatgg gaatcccgga accatccggt atggaaatgt caacggtaga
     5281 catgggggcc accacgtaga acggcacgtt gtggtggcgc gcaatgatcg cgaggccgta
     5341 cgtgccgatc ttgttcgctg cgtccccgtt cgcggcgatg cgatctgctc ccacgaacac
     5401 gccgtccacc ttcgtctgcc gcatcactgc cgcggccatg ttgtcgcaga tcaccgtcac
     5461 gtcgatccca gctcttgaaa gctcccacgc cgtcaggcga gcaccctgca gaagaggcct
     5521 cgtttcgtct gcgaataccc ggagtttctt ccccatttca gtcgccgcat agaacactcc
     5581 gagcgcggtg ccataccctg acgtagccaa tccccccgca ttgcaatgag tgaacacggt
     5641 tgcctcgtcc ggcagcagcg cggcgccatg gcggcccatc gcctcacatc tgcgagtgtc
     5701 gtcaacctcg atggcctgcg cctccttgag caaccctgtt acaaggtccc cgtccgctaa
     5761 tacgtcggcg acggacctca ttcgatccag agcccagaaa aggttcactg ccgtcggacg
     5821 cgttcgccgc agacgagcga tggccgcggc cactgcctcg cggcggtgcg aagggcctgc
     5881 gttcatcgca gcgatcacaa ccccgtacgc cgctgccaca cctatggccg gagcaccacg
     5941 gacctggagc gtttcgatgg cgtgggccag aacttcgacg tcggtacaga cgatgtatcg
     6001 ttcgtcgttc gggaggagcg tctggtcaag cagcctgagt ccgtcgccta cccattcaag
     6061 tgtcttgatc ttcatgcgtt gtggcgctcc tgaccgtgca gatcgggaag aaacgaaata
     6121 gaccgaagcc agcggtaccg ttcgtcaagc acttcgcgtg tggcatttct gagccggttg
     6181 aaggagtaat cttccacggc cagactcgcg gcagccgttc cgtaggcaag cgcccggcgc
     6241 aaaccggaga aggtgaaatc gcctgtcgcg gcgatgtacc cgagcattcc ccccgcgaag
     6301 gaatcgccgg ctcccgtggg gtcaaccacc gttttcagtg gaagcgcggg gaggacggag
     6361 aactcgtctt ccgccgcaag aagcaccccg tgctcgcctt ttttcactac taccgcacgg
     6421 ggacccatgt ccagaatcga acgggcggca accgtgagat tcgtcttccc cgtaagggcc
     6481 ctggcttccg agtcgttgac gactaccatg tggacgcgct gcagcacacg ggcaaggtcg
     6541 tctgccgcat cacggatcca catatccatc gtgtccagcg ccacccagtc cggaccggcc
     6601 atctggtcaa gcacgtgcaa ctgaagccgc ggcgcgatgt ttccgagaaa cacgactttg
     6661 tcgccgcggt acccttcagg aatgcgtggt gcgaaatccg caaagacatt gagttccgtc
     6721 gcgagcgtcc ggcggttgtt catgtcctct tcgtacacgc ccgtccaccg gaatgtcttg
     6781 ccctcagcag tttccaaacc tgccaggtcc acgccccttt cctcaaggaa cgcgatcccg
     6841 gcacggtcga aatcagtccc cacaatccct acgagatgaa ccggggcgaa cagcgacgcc
     6901 gcggcagcac caaaaagggc ggaaccgcca agcgcgtttt ccactctgcc accgggcgtt
     6961 tccacgttat catacgcgac tgaacccacg atgagaagcg aatcaggcat agtcgttttc
     7021 cttgacgttc aaacgtacac gcagaagccg caggagctgc ccggacagac ctgcggcttc
     7081 caggaacaca ccaaatccgg ctgtctgcgg cgaggtccgc gaagcaaact accggggacg
     7141 cctctcggtg ggcgatggtt gtccgccgac gcccgccgga accggcccga tgtaccccgc
     7201 ggagaacaga tcgagcccgg aaagctcccc gagatcaagc tcctcgatcg ggcgcgcaac
     7261 gttgacgccc agcgcaggcc cttcttcctt cgcctgcccg agaaccgtgc gcaaccggtt
     7321 ggggaacgga aggcctccaa gcatggtgta caggcggggc atctggtcgg gaggcccctg
     7381 gaaaagaccg gcgtaaatac cacttgtatc ggtgagttca cgaatctgac ggatcaggct
     7441 ctcgaagatg tccgccggcg cacgatcgag aagatcctgc ggcgccacaa ggatgatacc
     7501 cgccattgtc gcgtccttgt agtcgaaacc ggaggccaga agtcctgaag aatcccagat
     7561 gcttctcagg ccgttggcaa ggcgatctct gatcagcagg tcgtcgtccg tgagatccgc
     7621 ggccccgtag atgagcacgc cgcgcgaggc aaacacgcgc cggaaatcct ccccgtcgaa
     7681 accgcgaatc ggcatgtaca actcgttggt gctgagcgtg ttcatttcgt gcaggcgttc
     7741 caccgccgcc tgattcgcgg aggagtaaaa cgcggtcagg ctttcacttc ggtactgacg
     7801 caggatcttt tcgttgtcca cgagaattat gctggcgacg tccgaattgc ggaaaagatt
     7861 gatggcattg accgcgttga tcttcgcgat actgctctcg tggtctttcg gaagagaagc
     7921 cagcgcggaa atcgggatgt ccagccgcgc caggatattc gcaagcacgg cgatattgct
     7981 gcccgtgcct ccgccaaggc ccgcgcacag aagcaggtgt ctgcaccctt caagctgggt
     8041 acccaccgct tccacaatga tatcgctgct cgcctcgagg ctccgctgac ccagcgccat
     8101 gtcctgaccg gcgccgtcgc gcccttcgta cccgatgtag atgcgttttc tggggtccag
     8161 cgacagcgcg cgaaggtccg tatgtgaagt gttcaccgca acggctttgt acccgagcct
     8221 cgtgaactca acagccaggt tgccgccaca ttcgcccagt ccgacgatgc caaggtccaa
     8281 acacttctgc ccttctgaca tctactcctc ctctttcacg atcgcgggtt atccgcaggc
     8341 gggaggctcc ccgcgtgcag ggtttccccg acgcggggcg gaacgtccgg gaagggaacg
     8401 cgcggcgcat ccgcccacat ttcctccaga ccgtagaatt gacgtgcgtc ctctcgaaaa
     8461 atatgcacca ccgtgtccac gaaatccacg agcacccagt tgagggattc ttccccctca
     8521 acgtgccacg gatttcggcc aaacggcacc agagcctctc gcaccgcatc aaggatggcg
     8581 cgggcatgga catcgacgtc cgcggtgcac aacacgaaaa aatccgcgac gtcggtaagc
     8641 ccccgcagat ccatgactac aacattcgac gccttcttgc ttctgaccgc ctccacaaga
     8701 accccgaccg tttcgggaac gggggcggca tcgcgtgccg gtttccttcg tgaacgcgcc
     8761 tttttctgta tcattcaact tccggttgag gtccactcgc gcccgttcag gacggcatca
     8821 ccatcccgtg gacggatcat tccaccacgc gttgtaccca tagccgtacc cgtaccggtt
     8881 ggacggcatc tgcgagtaat gaacggacag caccatgttt tccgtcggcc ggtataccag
     8941 atcgagcgcc ggcatgacga tgctggcctg ttgcggaccg ttgcggaatc ccgccacacc
     9001 cagcggattg tgcaacaacc ccacatccat tcgcaaggtc agcggggtgg caatctggta
     9061 ctggatatgc tgaagcagaa gccctgcgga tgtcgtggtt cccccgctgc tggcgatgcc
     9121 gaacgagtag gaacgccacg tcgtcaactt gctggaatcg aacaacccct gacgtctcat
     9181 caggctgacg agatccgcgg tgggcgcgcc ggctatcgtg ggcccccgct cgccggtatt
     9241 ggtgacaaac ggccctgacg ccgtggctgt ctgcgccgcc agagcaagaa ctcccgccac
     9301 aagaatcgct acagttttca tcgctcgtat ccttctctcc ggggtgtggc acccatgccc
     9361 ttcaacccct tcctgtgaac gcgcggcaaa cccagcctgt tccgcgcata caacaggtca
     9421 tcaaagaata acaatctccg cggcaaactc aaatacgctg cgtgcacccg cggaggtcgt
     9481 aacgacgc
//
LOCUS       DAOSOO010000117         9402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7267_length_9402_cov_5.242431, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000117 DAOSOO010000000
VERSION     DAOSOO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_9402_cov_5.242431"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PLT86_09165"
     CDS             <1..1089
                     /locus_tag="PLT86_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT36821.1"
                     /translation="RLHGEPIRMITFPLPEYNGSDAAWGQVALSLHDLDRARKKNQLA
                     LAIILPAAIGISAVAGWWLARGALRPIDGVIRTAQRIRAANLNERIAPREVDDELGRL
                     ISTLNELLERLELNFRQVSRFSADVSHELRTPLTIIQGEAEVALREGASREEMQLALE
                     VALDEAQRMSKLVKNLLMLARLETGQERPHFVETPLAPIIEDVSEEGLALARAKGVTF
                     SAHTVENASVWGDAVLLHQLAFNLVDNAVKYTPAGGTVELSLSRNGSSVRLSVKDTGV
                     GIDEAELGRIFDRFYRGDSARNHTEGGSGLGLALVAQIAEAHKGTIQVTSKPGEGSVF
                     TVTFPLASQAGKARPDMAEVSMIEGIRS"
     gene            1086..2516
                     /locus_tag="PLT86_09170"
     CDS             1086..2516
                     /locus_tag="PLT86_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT36822.1"
                     /translation="MIETAVILAAGNGSRLRGVSAGLPKPLVPLAGVPIIVRVMRAAQ
                     EAGIRKFVVVTGYQAEKLRAAIENQPAITASITWVHNPEYKTRSNGTSALAVAGAVDG
                     PFVLLMADHIFEVATLRRLLRTPIGPDEVILAIDRKIDRVFDLDDATKVVDDNGKLRA
                     IGKQLPDFNAIDTGMFLCTPALFDALRAVQDESDGSLSDAIRRIAAENRMRTFDIGDA
                     LWQDVDTPEMRREAERRLGDALRKPTDGPVSRLINRRISIPLSLFLTRTRVTPNQISV
                     FNLLLAACGGLLFASTSYPILALGGILLQVGSILDGCDGEVARLKFQQSKNGQWMDTV
                     TDNISYVALLVGLAIGQYRREPTSMTLWLGFLAIAAILTGLFIVYQRIHAMGKGSLLD
                     FKIPDRSRVDPTTARIFWIYEQLLPLIRRDIFAFGCCLLALANLPSVIFSLWVLGSVG
                     FMFGVIHITSPHVQRQLAPVPAVAKD"
     gene            2526..3698
                     /locus_tag="PLT86_09175"
     CDS             2526..3698
                     /locus_tag="PLT86_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT36823.1"
                     /translation="MRVCLDARCLRKTVTGLGRYAEQLIRGLAAIDRTTDYVVILAKD
                     YECQPVEQASFQIVRFGGRPDTLGNVLGGARKIAGLDADIFHSLHHFLPFGVRGRTVL
                     TLHDFIWLDHPDLAYPNRWKGRVIQAYASLALNYALRRANHIIAISEHTARRARERMA
                     PRIPEISVIPHGVDERFHRAAKRHASGFATTEAPFFLVVGNSKPNKNVARIVGAMALL
                     VQKVPEVRLRIVGRGEGYRELRSQVDSLGLTQKVDFYGNVPDEELLDALQRAVALVFP
                     SLVEGFGMPVLEAQAVGCPVITSDRGVLPETAGDAALFVDPYDAWDIARAMETLYRDL
                     PLRESLVRKGRERATRFTWEETAKKTRHVYDLVMGQSLPEFHAVRQVLAPAAGVQA"
     gene            3699..5222
                     /locus_tag="PLT86_09180"
     CDS             3699..5222
                     /locus_tag="PLT86_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT36824.1"
                     /translation="MSPAARTHRSDAALLARGAIVNLLGNIGRLSRAFSLMLIAWLFG
                     AEALGLYVLAWATTEFLRRFVSYGIEPTIVYKLGPLLVSHDDTRIAQTIASSLVLVGV
                     TSLVGSLALYFGAPFIANTVLREPRLVEVLPVFVFAMPAFAVTSVFLAATRAKKIMRF
                     EIIARSFVEPFVLLLGTLVAWAAKTGVTGLVVAQTSAFWISAFFCAYFYGTQFPLRAL
                     FAALRRPSHLAGVFHFATPVAAKDVIAQGVARIDLFLVGHLLDAKAVGVYGIVLELVV
                     FSKHARQALEPIMAPLVAEQHHTADRERLHRTYANATRWALLVNLGLFGVAQIAGDSV
                     LSIYGKDFKAGGTALAILFFGQIVSGTFGLSETVLLMVGRPALMLGDMALLLAMIGVF
                     DYAAIQVWGLTGAALGTAGATMLVVLLQVRQARKKAGVHPWSRALLKPVGACALGLAV
                     AHLLPLGELDGITSDVVKTATFSAIYSLLLVWLGMEAEEKHLGAWVKAKLHSLLNEG"
     gene            5454..5840
                     /locus_tag="PLT86_09185"
     CDS             5454..5840
                     /locus_tag="PLT86_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36825.1"
                     /translation="MRRLVWVLALALSAMVAACGGGQQEIGTTARLGGSNTRIRIVNG
                     LGSYTIRYVYISPASDDSWGSDRLGASQVLRPGQSETWDIDPGTWDLRVKDEDGDTYT
                     KRGIRLSSGKTYVWTVRLSDLDRRRR"
     gene            complement(5837..6469)
                     /locus_tag="PLT86_09190"
     CDS             complement(5837..6469)
                     /locus_tag="PLT86_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36826.1"
                     /translation="MSFGVCRPFAINELALGYAGGGYSSNRWGVSGAVDSWGWEVYRE
                     SRTTLAIGRKMGKRWSLGTSIRTKWVQIEHFRSSGVADLTLDVCANPVAGLRVYGQVT
                     GIARNNWGEALLNTPRSVLCAADWKVGLVDLRGAVTRETSNVHTTDVSASFRFYPWLS
                     FTVARASGPAAYLFGVEIGRSHAAGYSLSLHPVLPATQTMRFREIVRRRG"
     gene            complement(6664..7080)
                     /locus_tag="PLT86_09195"
     CDS             complement(6664..7080)
                     /locus_tag="PLT86_09195"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOT36827.1"
                     /translation="MRPDSLSNSVQRTPAGAFKRSLLFPGWGQRYNGKPWKGAVYTGI
                     VAASAAMAAKNWDTALGTTTAGSLFWSSSAWTRGRNNWLILGAFAYALAGVDAYVDAH
                     LNTFDVGPITAQPVVTRDRRSVLVAFRVPLSFLERQ"
     gene            complement(7155..8114)
                     /locus_tag="PLT86_09200"
     CDS             complement(7155..8114)
                     /locus_tag="PLT86_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT36828.1"
                     /translation="MSVFTIGIDLGGTNVRAGVVTEDGQVISSCGHPTEVSKGQEHSI
                     RQIVRTAEEAVERAGISRQDISAVGIGSPGPLDPFNGLVLSPVNFPTWGTVPLAQILS
                     DAFHGVPTALDNDANVVAYGENWLGAGRDTANFLCITLGTGVGGGVVCEGKLLHGSDG
                     NAAEIGHISIDYNGPRCNCGGIGCLELYCSATAIARRTRAAMEAENPKTLLTGNEVTA
                     QTIADAANAGDEFARRMYDETGFFLGMGIVSAVALFNSEIVAVGGGMARAGDLIFEPA
                     RRTFHEFGFPSLRGRLRIEPMRLGDNAAILGAARLAMDGGIRR"
     gene            complement(8132..9172)
                     /locus_tag="PLT86_09205"
     CDS             complement(8132..9172)
                     /locus_tag="PLT86_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36829.1"
                     /translation="METLRVGIVGTGSIGMVHLDTYAKIPGYKVTAVCDVRETALAQA
                     RAKSGAAGFADYHDLVTQNDVDVVAVCTPNYLHAPVTIAALNAGKHVFVEKPMAYDVK
                     AAKEMIAARDKSGKLVQIGVCQRFRGDAQVLKSHIENGELGRIYFAKCGYLRRSGIPG
                     MGSWFTTRSQSGGGPILDLGVHALDLTMYLMDNFKPVGVMACKYAEFGPRGLGAGGWG
                     TAVPGGPFDVEDLAAALIKFENGATIFLEVSWAAHVGSGSFYSTLMGDKAGANLDPAT
                     IYTDEMGHPVDKKLTVPSVDAYHAEHGHFHDCIVNNKKPMPSVEQGLAVQAVLDGIAE
                     SADKGTSVTISL"
BASE COUNT         1816 a         2773 c         2821 g         1992 t
ORIGIN      
        1 cgtctgcacg gggaacccat ccgcatgatc acgtttccgt tgcccgaata caatggaagc
       61 gacgccgcgt ggggacaggt tgcactttcg cttcacgacc tcgaccgcgc cagaaaaaag
      121 aaccaactgg cgcttgcgat tatcctgccc gccgcgattg gcatttcggc tgtggcgggt
      181 tggtggctcg ccagaggcgc acttcggcct atcgacggcg tgatcaggac tgcccagcgg
      241 atacgtgccg cgaacctgaa tgagcgaatc gcgccgaggg aggtagatga cgagctggga
      301 cggctgatca gcacgctgaa tgaactgctg gagcgtctcg agctgaactt ccggcaggta
      361 agcagattca gcgccgacgt atcgcacgaa ctgcggacgc ctttgacgat cattcagggc
      421 gaggctgagg ttgcattgcg cgagggcgcc agcagggaag agatgcaact ggcacttgaa
      481 gtggcgctgg acgaggcaca gcgcatgtcg aagctggtga agaatctcct gatgcttgcg
      541 cgccttgaaa cgggtcagga gcgacctcac ttcgtggaga cgccccttgc gccgattatc
      601 gaggacgtga gcgaagaagg acttgctctc gcccgcgcga aaggcgtcac gttttcggca
      661 cataccgttg aaaacgcctc cgtctggggt gatgccgttt tgctgcatca actggcgttc
      721 aaccttgtgg acaatgcggt caagtacaca cccgcaggcg ggacggtgga actcagcctc
      781 tccagaaacg ggtcaagcgt acgtctttcc gtgaaggaca ccggcgttgg aatcgacgaa
      841 gccgaactgg gtagaatctt cgaccgtttt taccgtgggg acagcgcacg caaccatact
      901 gagggcggaa gcggcctcgg gctggcgctc gtggcacaga tagcggaagc gcacaaaggc
      961 accattcagg ttaccagcaa accgggcgag ggcagcgtct ttaccgtgac gttcccgctc
     1021 gcgtcacaag ctggaaaagc gcgaccggat atggcagaag tctcgatgat tgaagggatt
     1081 cgttcatgat tgaaacagcc gtgatactcg cggcaggtaa cggaagtcgt cttcggggtg
     1141 tctccgcggg cctcccgaaa ccgctggttc cgctggccgg tgtgccgatc atcgtcaggg
     1201 taatgcgcgc cgcacaggag gccggtatac ggaaattcgt ggttgtgacg ggttaccagg
     1261 cggaaaaact ccgggctgcc attgagaatc agcccgccat tacggcgagc atcacgtggg
     1321 tccataaccc cgagtacaag acgcgctcca acggcacgtc cgcgcttgcc gttgcaggcg
     1381 ccgtggacgg cccctttgtg ttgctcatgg ccgaccacat tttcgaagtg gccacgcttc
     1441 gccgtctgtt gaggacgcca atcggcccgg acgaggtgat tctggccatt gaccggaaga
     1501 ttgaccgtgt ctttgacctc gatgacgcga cgaaggtggt agacgacaac gggaagctgc
     1561 gcgccatcgg caaacagctt ccagacttca atgccatcga caccgggatg tttctgtgca
     1621 ctcccgcgct ctttgacgct cttcgcgccg tgcaggacga gagtgacggc agtctttccg
     1681 acgcgatacg tcgcattgcc gcagaaaacc ggatgcgcac gtttgatatc ggcgatgcgt
     1741 tgtggcagga tgtggacacc cccgaaatgc ggcgggaagc agagcgccgc ctgggagatg
     1801 ccctgcggaa accgacagac ggccccgtga gcagattgat caacaggagg atctccattc
     1861 cgttgagtct gttccttacg aggacgcggg ttactccaaa ccagatctcc gtgttcaacc
     1921 ttctgcttgc cgcgtgtggc ggtttgctgt tcgccagcac gtcgtacccg attctggccc
     1981 tcggaggaat tctcctgcag gtcggttcga tcctcgacgg gtgcgatggc gaggtagcgc
     2041 ggctgaaatt ccagcagtcg aaaaacggtc aatggatgga cacggttacg gacaacattt
     2101 cgtatgttgc tctcctcgtg gggcttgcga tcggtcagta ccgtcgggag cctacgtcca
     2161 tgaccctgtg gctcggtttt ctggccatcg ccgccattct gacgggactc ttcatcgttt
     2221 accagcgaat ccacgcgatg ggcaaaggca gtcttcttga tttcaagatt cctgaccgta
     2281 gccgtgtgga cccgacgaca gcgcggatat tctggattta cgagcaactg cttcctctta
     2341 tccgccgcga catttttgca ttcggctgct gtcttctggc gctggcgaat ctgccttccg
     2401 tgattttttc gctgtgggtt ctggggtcgg taggcttcat gttcggggtt attcacatca
     2461 ccagcccaca cgttcagcgt cagcttgcgc ctgtcccggc agttgcaaag gactgaaagg
     2521 tttgcatgcg cgtctgcctc gatgcccgtt gtcttcgcaa aaccgtgaca ggcctggggc
     2581 gttacgcgga gcaactgatt cgtggtctcg ccgcaataga tcgcaccacg gattacgtgg
     2641 tcatactggc aaaggactac gagtgtcagc cggtcgaaca ggcgagtttc cagatagtgc
     2701 gcttcggcgg gcgcccggac accctcggta acgttctcgg gggcgcacgg aagatcgcgg
     2761 ggctggacgc ggacattttt cactctctgc atcattttct ccccttcggt gttcgtggac
     2821 gcaccgtcct cacgcttcat gacttcatct ggctcgatca ccctgatctc gcctacccga
     2881 accgctggaa ggggcgggtg atccaggcgt acgccagcct tgccctcaat tacgcgctgc
     2941 ggcgagcgaa tcacatcatc gcaatttcgg agcacactgc ccggagggcg cgggagcgta
     3001 tggccccgcg aattccggag atatcagtga tccctcacgg ggtggacgaa cggtttcacc
     3061 gggcagcaaa aaggcatgct tccggctttg ccaccacgga agctcccttt ttcctggttg
     3121 tcgggaacag caaaccgaac aagaacgtgg cccgcatagt cggtgcgatg gcgttgctcg
     3181 ttcagaaggt tccggaagtc aggctccgca ttgtcggcag gggtgaaggg taccgcgagt
     3241 tgcgcagcca ggttgattcc ctcggcctca ctcagaaggt ggacttctac ggaaacgtgc
     3301 cggacgagga gctgcttgat gccctccaga gagctgttgc gctggttttt ccgtcgctcg
     3361 ttgaaggttt tggcatgccc gttctggaag ctcaggcagt cggctgcccg gtaatcacca
     3421 gcgaccgcgg agtcctgccg gaaacggcgg gagacgcagc cctgttcgta gacccgtatg
     3481 acgcctggga tatcgcccgc gcgatggaga ctctttaccg ggacctccct ctgcgggagt
     3541 ctcttgtacg gaaaggtcgg gaacgcgcca cccgcttcac atgggaagag accgcgaaaa
     3601 agacacgaca cgtatacgac cttgtgatgg gccaatctct tcccgaattc cacgcggtac
     3661 ggcaggttct tgcgcctgcc gccggtgttc aggcgtaaat gtctcccgca gcacgcaccc
     3721 acaggagtga cgccgcgctt ctggcacgcg gcgcaattgt aaacctcctc ggcaacatcg
     3781 gccgtctttc ccgcgcattc tccctcatgc tcatcgcctg gcttttcggc gcggaagcac
     3841 tggggttgta cgtcctcgcc tgggctacga cggaatttct acgccggttt gtcagctatg
     3901 gcattgaacc gacgatcgtg tacaagctgg gtcccttgct cgtatcgcac gacgacacac
     3961 gaattgctca aacgatcgca tcgagcctgg tgctggtcgg cgtaacctcg ctggttggca
     4021 gtctggcgtt gtattttgga gcgcctttca tcgcgaatac ggtcctgcgc gagccacggc
     4081 tggttgaagt cctgcctgtt ttcgtcttcg ccatgcctgc gtttgcggtc acttccgttt
     4141 tcctcgctgc gacccgcgcg aagaagataa tgcggttcga aatcatcgcg agatcgttcg
     4201 ttgagccatt tgttctcctg ctgggcacgc tggtggcatg ggctgccaaa acgggtgtga
     4261 cagggttggt cgtagctcaa acctcggcgt tctggatttc ggcgtttttc tgcgcgtatt
     4321 tctacggaac tcagttcccg ctgcgcgcgc tgtttgcggc gcttcgccgg ccttcgcatc
     4381 tcgcgggggt gttccacttt gccactccag ttgcggcaaa agacgtgatc gcgcagggcg
     4441 tcgcaagaat tgatctgttt cttgtcggcc atcttctcga tgcgaaggca gtgggtgtgt
     4501 acggcatagt tctcgagctc gtggtgtttt cgaaacatgc ccgacaggcg cttgagccta
     4561 tcatggcacc cctggttgcc gaacagcatc acaccgcgga ccgtgaacga ttgcaccgaa
     4621 cctacgcgaa tgccacacgg tgggcattgc tcgttaatct cggacttttc ggagttgcgc
     4681 agatcgcagg agactcggtg ctctcgatct acggaaagga cttcaaagcc ggcggcaccg
     4741 cactggcaat cctctttttc gggcagatcg taagcgggac gtttggcctt tcggaaacag
     4801 tgctccttat ggtgggacgc cctgctctga tgctgggcga catggcgctt cttctcgcga
     4861 tgatcggagt tttcgactac gcggccattc aggtttgggg tttgacgggt gccgccctcg
     4921 gcaccgccgg cgcaaccatg ttggttgttc ttctgcaagt gcgacaggca cggaagaagg
     4981 cgggtgttca cccctggagc agggcactgc tcaagcccgt gggcgcgtgc gcacttggcc
     5041 tcgcagttgc acatctcctc cctctggggg aacttgatgg gatcacgtcg gacgttgtga
     5101 agacagctac tttttccgct atctacagtc tcctgcttgt ctggttgggc atggaagcgg
     5161 aagagaaaca tctgggagcg tgggtaaaag cgaaactcca ttccctgctc aacgaagggt
     5221 gacgcagggc aatcgccgcg atgagcactg aaggttgtgc gcggcgaaaa aaaaatacaa
     5281 cgccacaggc cggaaccact atccaatcgc cggtgttgcc agtatctttt ttgtgagagc
     5341 gattgcacct gcgcccggaa tttcactgcc acgctgtgga acctcggtgt tgcccgcggc
     5401 gccaagttcg taccaatgcc gatgagatcg gcgcacagag gagagggtcc ggaatgcgac
     5461 gtctggtgtg ggtgttggcg ttggcactgt ctgcgatggt agcggcctgc gggggcggtc
     5521 agcaggaaat cggcacaacc gcccgtctcg gtggcagcaa cacgcggatt cgaatagtca
     5581 acggtctggg aagttacacg atcaggtacg tgtatatttc gccggcctca gacgacagct
     5641 ggggaagcga ccgactcggc gcaagccagg ttctccgtcc cggccagtcc gaaacgtggg
     5701 atattgaccc ggggacgtgg gatctgcgtg taaaggacga agacggcgac acgtacacga
     5761 agaggggaat ccgcctgagc agcggcaaaa cgtacgtgtg gacggtccgc ctgtcagacc
     5821 tcgacagacg acggcgttaa ccccgcctgc gcacgatttc cctgaatcgc atcgtctgcg
     5881 ttgccggtag aaccggatgc agagacaggg aatacccggc agcgtgcgaa cgtccgatct
     5941 cgacgccgaa caggtacgcg gcgggaccgg acgctcgcgc gacggtgaac gacagccacg
     6001 ggtagaagcg aaatgatgcg ctgacatcgg tggtatgaac gttactggtt tccctcgtga
     6061 cggcacctct gaggtcaacg agccctactt tccagtctgc tgcgcacaga actgaacgcg
     6121 gcgtgttcag gagcgcttcg ccccagttgt tgcgggcaat acccgttacc tgaccataca
     6181 cgcgcaggcc cgcgaccggg ttggcacaca catcaagagt gaggtctgcc acgccggacg
     6241 accggaaatg ttctatttga acccatttcg tacggattga agtgccaaga gaccaccttt
     6301 tgcccatctt tctgccaatc gccagcgttg ttcgcgattc tctgtagacc tcccagcccc
     6361 agctatctac tgcccctgat acgccccaac ggtttgagga gtaaccaccg cccgcgtatc
     6421 ccagagccaa ttcgttaatc gcaaaagggc ggcatacacc aaacgacacc atcgcttcct
     6481 ggctgtttcc tggtaacgcg gcgggcctgt tccagggaga gagaccgtct gcgaagcgca
     6541 aaggcaaagc ttcaccaagg aaggtatttt cggggacgaa tgcggcgctc gccggcgagg
     6601 ggagagagaa caccgccgtc aggaatgcaa cctgcacgat tcggaggttg ccggtcgcgg
     6661 atttcactgt cgctcaagaa aggagagtgg aacgcggaac gcgacgagga cgctccttcg
     6721 gtcccgggtt accacgggtt gagcggttat cgggccgacg tcgaacgtgt tgagatgcgc
     6781 gtcaacgtat gcgtcgacgc ccgccagcgc gtaggcaaac gcgccgagga tcagccagtt
     6841 gttccgcccc cgggtccacg cggaactgct ccagaacaga cttcccgcgg tagtggttcc
     6901 gagtgcggtg tcccagtttt tcgcggccat agcggcgctt gctgccacga tgcctgtgta
     6961 caccgcccct ttccacggtt tcccgttgta ccgttgtccc cagccaggga agaggagcga
     7021 gcgtttgaat gctcccgcag gcgttcgttg gaccgagttg ctcaggctgt cgggacgcac
     7081 ttcctgcgaa aaagaacgcg gcacgtacgc gaacgagagc gcaagtgccg cgtaccagac
     7141 tatcgcccgt cttttcagcg gcgaatccct ccatccatag ccagccgcgc cgcaccgaga
     7201 atggcagcgt tatccccgag gcgcatcggc tcgatgcgca gccgccccct cagcgacggg
     7261 aatccgaact cgtggaaggt ccgacgcgcc ggttcgaaaa tcaggtcccc cgcacgggcc
     7321 atgcccccgc cgaccgcgac gatttcgctg ttgaaaaggg cgaccgcact tacgatgccc
     7381 attccgagga agaatccggt ttcatcgtac attcgtctgg cgaattcgtc gcccgcgttg
     7441 gcggcgtccg cgatggtctg cgcggtcact tcgtttcccg tgagcaacgt tttcgggttt
     7501 tctgcctcca tcgcggcgcg agttcgtcgc gcgatggcgg tggcgctgca gtacagctcc
     7561 agacatccga ttccgccgca gttacaccgg ggaccgttgt agtcgatact gatatgtcca
     7621 atttccgcgg cgttaccgtc cgacccgtga agcaacttcc cttcgcacac gactcccccg
     7681 ccgacacccg ttcccaacgt gatgcagagg aaattggccg tgtcgcgtcc tgcccccagc
     7741 cagttctcac cgtacgccac cacgtttgcg tcgttgtcga gcgcggttgg aacgccgtga
     7801 aatgcgtcgc tcagtatctg cgcgagcggg accgtgcccc acgtcgggaa attgacaggc
     7861 gaaagcacca gcccgttgaa tggatccaac ggccccggtg atcctatccc gacggcggag
     7921 atatcctgcc tggatatccc tgcgcgttct actgcctctt ccgccgtgcg tacgatctgg
     7981 cgtatggagt gttcctgacc cttgctgacc tctgtcgggt ggccgcaact cgagataacc
     8041 tgtccgtctt ccgtcaccac tccggcgcgg acattcgtgc caccgagatc tataccgata
     8101 gtgaacactg acatggaact cccggtggcg gttagagcga aatggtaact gacgtgcctt
     8161 tgtctgcaga ttcagcgata ccgtcgagca cggcctgaac agccaatccc tgttcgacgc
     8221 tcggcatcgg ctttttgttg ttcacgatac aatcgtggaa gtgaccgtgc tccgcgtggt
     8281 aggcgtccac ggatggaacg gtgagctttt tgtccacggg atgacccatt tcgtcggtat
     8341 agattgttgc cgggtcgaga tttgctccgg ctttgtcacc catgagggtt gaatagaagc
     8401 ttcccgaacc gacgtgtgca gcccagctta cttcgaggaa gatcgtcgcc ccgttctcga
     8461 atttgatgag cgcggctgcg aggtcttcga catcgaacgg gccacccggg actgccgtgc
     8521 cccagccacc ggcacccaga cctctcgggc cgaactcagc atatttgcac gccatgacgc
     8581 cgaccggctt gaagttatcc atgagataca tcgtcaggtc aagtgcatga acgccgaggt
     8641 cgagaatggg tccgccgccg ctctggcttc gagtagtgaa ccagcttccc atgccgggga
     8701 tgccgctgcg ccggagataa ccgcatttgg cgaagtaaat gcgtccgagt tcgccgtttt
     8761 caatgtgcga tttgagaacc tgggcgtcgc cgcggaagcg ttgacagacg ccgatctgga
     8821 ccaactttcc ggatttatcc cgcgcggcga tcatctcctt cgcggctttg acatcgtatg
     8881 ccatcggttt ctcgacaaac acgtgtttgc cggcattgag ggcggcaatt gtcaccggtg
     8941 cgtggaggta gttgggcgta cacacggcga cgacgtctac gtcattctgc gttaccaggt
     9001 catggtaatc cgcaaaaccg gcggcgccag atttggccct ggcttgggcg agtgcggtct
     9061 cgcggacatc acacaccgcc gtaactttgt accccgggat ctttgcgtac gtgtccagat
     9121 ggaccatccc gattgagccg gtccccacaa ttcccacgcg taacgtttcc atcttcgttc
     9181 tcccctgtgt cgacagttat gacggttatt cacatgttct cagaacaaca aatcagttgt
     9241 aaagctacgg acggagccag tttgtcgcaa ttgcctttgc cggattcgag ccggtcacgg
     9301 acgcttccag ctcgcgattt tctccccgag tacctgaacc agacccgcga ccgggtatcg
     9361 ctcctgaagc atcgtgtcgc gatcacgcaa cgtgacggta tc
//
LOCUS       DAOSOO010000118         9352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7305_length_9352_cov_5.831881, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000118 DAOSOO010000000
VERSION     DAOSOO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_9352_cov_5.831881"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLT86_09210"
     CDS             complement(<1..1397)
                     /locus_tag="PLT86_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HOT36830.1"
                     /translation="MKALRGPTTWWFLAVLVTLVPFGTSWAAGTPAGTVISNFATLSY
                     KDANGNALPNINSNTVTTVVAQVAGVDVSPATSAKSVTENSWVSYTATITNMGNGTDT
                     FALTTSALSSGWTAVIYGDPNGNGILDAGEMVPGNIVSTIASLPADSSKKVFVVVNVP
                     TGALDGSSQAVTLTATSQFNTGVSDAGVFTSTVSTAVLSMVKTASPSDPKPGDVVTYS
                     IRGNNTGTSTAYGIVFSDVVPSGMTYVPGSIRIFAGSGGTYATATPQTDATGDDSADF
                     GITAANTVTINWGDSPAGQEGWLFFQATVNAGVAAGTSISNVASVLYSSTSGGPLLPA
                     VSSTPGDITVAAKPYVTLSNTSLALTGQPGDTLLYRFSVTNGGNSNDVFDFTTSSTQG
                     FTNQIWLDANNNGIAGDDGDVLLTDTDGDGKVDTGSIPAGTVAHIIVAVVVTPGTVDM
                     TVDATTVTIASSVDPT"
     gene            complement(1487..2056)
                     /locus_tag="PLT86_09215"
     CDS             complement(1487..2056)
                     /locus_tag="PLT86_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36831.1"
                     /translation="MGSTMRTRRSGTVLVLLIAVCAALAVSEPATGQQPAPNGVSITI
                     TDELVLPAGDARIGKAVRDSAGNLISKPGDIIRYTLTAVNNGTEPAYGVEIVDPIPRG
                     TEYVIGSAAGVGMTITYSIDGGRFFQPPPIMYDFQNPDGTIEKRHVPADRYTHIKWLV
                     NEPIRPRSKAVATVRVRVSLPGAAEGEGQ"
     gene            complement(2071..4077)
                     /locus_tag="PLT86_09220"
     CDS             complement(2071..4077)
                     /locus_tag="PLT86_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36832.1"
                     /translation="MPSSEILFPRSVRVPSTRRRFPLFPALVAVLLVAASAVPAALQN
                     YRDPTGYTYPTTWPSVLATSAYTRLGVPEADKTGQTDGSKGASPQGANDFTSGPLYDQ
                     PSFYYYGNGTILYFRFRVGGPPTALTGSGQPFTSATWNVLMDIDGDGWKEFVVFLDGT
                     SSGNQPDDIVIIYENVNSQKFDMNQVGVWRQDCAGPSDGVDGASGSSSTWDTDPDAYV
                     WDFGRTRVVQIDRTKPVGSQKSEYWIDVQVPLEAFDGRANGGPLLTGSSFFSISATTS
                     ASNSDPTQKDLLYSGDFALADVPLPGGDIYNGYGGRIDAPIIAELTQTGCPSPDTLKA
                     RVSDVLTVVGGLTTDTIDSVWFEYYYDYNGDWGENDPGQTWTRIGAGTRTGIGQFRYV
                     WDVSQMSAGNFLIRAVARDKQGNTTYSTSNPAGTAYLIAKFTNTCSAIAVLNTSTKSV
                     SDLNGGLTLPADTLLYTIVIQNTGGGTATSVVLKDTVSTYLNYVPNSATPTPAATNPA
                     LQWNVGTLAAGASATFTFRGVVKSPIANQTEILNKGWITYNTGAMTGLSKLVTATITV
                     TSQPIATCSKTVDKATAAPGDTLVYTVTYGNNGTDAATFLIISDTVPFETDYVPNSVV
                     LNGVPKTDAADADQVTVSGNNVSVTVGTLPVGGSGTFSFRVKVK"
     gene            4721..5563
                     /locus_tag="PLT86_09225"
     CDS             4721..5563
                     /locus_tag="PLT86_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT36833.1"
                     /translation="MTTRKTLNHVVVAVKQVPDTTQVKVDPATGTLIREGVPFIMNPF
                     DTHAVEEALRLKDRFGCLVTAVTMGPPNAAATLRKCLALGVDDVVLVSDRVFGGADTL
                     ATSYVLSEVIRRVEKEQGRVDLVICGKQTIDGDTAQVGPGIAVRLNYCLLTLVDRILD
                     ISGAERTITVRRKLEGRREVVRALMPACLTLVREVNEPRYPKVPNRLMAETAEIPVWD
                     NQFLQLSLSRVGLKGSPTHVKKIFAPERARGEILGDAENDPEGAVALLIRRLIEKDLI
                     SVGR"
     gene            5631..6968
                     /locus_tag="PLT86_09230"
     CDS             5631..6968
                     /locus_tag="PLT86_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT36834.1"
                     /translation="MGEPKRPKRPRGRARLLEGKCIACGALCQAECPADAIEMNDRGE
                     PIIILENCIGCKKCVKVCPASALEMFFTPEEEAVLAELARLGFAKEAPEKEGPVPKVD
                     QSYRGVWVFVEQQNGRAAAVSWELLGAGAKLAADLEVDLCAYVLGHEIGHLADEAFAY
                     GAKKVYLVDDPILKNYRTEPYLKASSDLIRKYKPEVVLMGATGLGRDLAGAVATDLHT
                     GLTADCTGLAIDKAGRLLEQTRPAFGGNIMATILTERHRPQMATVRPHVMPKPEPVSG
                     ARGAVVVEPLDMSEDDIRVKVLEVREDEGAANRVDIAGAEVLVSGGRGMQGPENYRLL
                     QELADVLGGTVASSRAAVDAGWMPYERQVGQTGKTVRPKIYIACGISGAIQHLVGMQD
                     ADLIIAINRDKDAPIFEVAHFGLVGDLFQIVPLLTKRFREMAGKPSDSGNEKG"
     gene            6974..8974
                     /locus_tag="PLT86_09235"
     CDS             6974..8974
                     /locus_tag="PLT86_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT36835.1"
                     /translation="MSQTENVIFAVVLAIAVAAFLQSAWRLVSFIRLGKPDGRLSGEL
                     VRRFWTMLVYAFWQRRVRKEPYGWNHFFLFWGFIILFLANGEFVIAGLFPSLTFELLG
                     ERIYGVLLFLFDVVSMIVLVNVAIAFFRRLVLKPAHIEYNSRDGYLILSLIGALMLAF
                     FCLYGSEFASGTMSVAGVMPFTETIFAPLMGSLFGSAAPIAARVFWWLHGLIVLSFLN
                     YLPYSKHMHILTAIPNCYARSFEYPATVPREEFKPGAQFGVSVVDEFSWKDLLDFTAC
                     TECGRCNQNCPATSTGKPLNPRLLVHDGKVNLLTNGPKLLQGNRSANLLPLIGTTDEH
                     EGTVGEDALWACTTCGACMTNCPVFIEHVPKIIKMRRHLVENEARFPHELSVFFESVE
                     QRSNPWGITPGDRAKWARDLSVPLMSDAKCDTLFFVGCAGAFDARNKKVATAVVRALK
                     AAGVEFAILGTEEKCCGDSVRRLGNEYLFERMAKANVELFKKYDVRRIITYCPHCYTT
                     LKHDYRQFGMETQVVHHSEFLHELLETGRLKLDGNKPAVDGKVLYHDSCYLGRYNGLY
                     EEPREVVQRVTGRAPVEMDRRLEKSFCCGAGGGRMWMEEEPEHRINANRVREALGKDA
                     DVIAVACPYCMTMFDDGIKDERAEGRVKVMDIAELVAARLPG"
     gene            complement(9115..>9352)
                     /locus_tag="PLT86_09240"
     CDS             complement(9115..>9352)
                     /locus_tag="PLT86_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36836.1"
                     /translation="LGGSRVTCAPRTSSGKGASRGVWLGSLSQAWQGYKVEEAVNEAL
                     VLFRPVLVLFCHVSDYSRPDPTIALIERRRMNSA"
BASE COUNT         1791 a         2699 c         3027 g         1835 t
ORIGIN      
        1 gtcggatcaa cactcgatgc aatcgttacg gtggttgcat cgaccgtcat gtccaccgta
       61 ccaggggtca ccaccaccgc cacgatgatg tgggcaacgg tgccggccgg gatgcttccc
      121 gtatccacct tgccgtcgcc gtcggtgtcc gtcaacagca cgtcaccgtc gtcgccggcg
      181 attccgttgt tgttggcatc cagccagatc tggttggtga agccctgcgt ggacgacgtg
      241 gtgaagtcga atacatcatt cgagttgccg ccgttcgtca cgctgaagcg gtacaacaac
      301 gtgtcgcccg gttggccggt gagcgccagc gaggtgttgg aaagcgtgac gtacggcttc
      361 gccgccacgg tgatgtcacc cggcgtactg ctcactgccg gaagtagagg gccacccgat
      421 gtggagctgt acaacaccga ggcaacgttc gatatgctgg ttcccgccgc aactcccgcg
      481 ttcacggtcg cctggaagaa aagccagcct tcttgaccgg ccggggagtc accccagttg
      541 atggttaccg tgtttgccgc ggtgattccg aaatcagccg agtcatcccc ggtggcatcc
      601 gtctgcggcg tagccgtcgc atatgttccg ccggagccag cgaaaatgcg aatagaaccc
      661 ggaacgtacg tcatgccgga cggcaccacg tcggaaaaca cgatgccgta cgcggtgctg
      721 gtgcccgtgt tattgccacg gatggaatag gttacgacgt cacccggctt cgggtcggag
      781 ggagaagccg tcttcaccat gctgagtact gcggtcgaca ccgtggaggt aaagactccc
      841 gcgtccgaca cacccgtgtt gaactgggag gtggccgtca gtgtcaccgc ctgggacgag
      901 ccgtcaagcg cgcctgtcgg tacattcacg acgacgaaca cctttttcga gctgtccgcc
      961 ggaaggctcg cgatggtgct gacgatgttc cccggcacca tttcgcccgc gtcgagtata
     1021 ccgttgccgt tcgggtcgcc gtagatcacg gcggtccagc cggacgacag ggctgacgtg
     1081 gtgagagcga acgtgtcggt gccgttcccc atgttggtga tcgtcgcggt gtacgagacc
     1141 cacgagttct cggtgaccga cttcgccgaa gttgccggag acacatcaac gccggccacc
     1201 tgcgccacca cggtggtgac ggtgttcgag ttgatgttcg gcaacgcgtt gccattcgcg
     1261 tccttgtagc tcaaggtcgc gaagttgctg atgacggttc cggcaggcgt accggcggcc
     1321 caggacgttc cgaaaggaac cagggtcacg agtaccgcca ggaaccacca tgttgttgga
     1381 cccctgagtg ccttcatggc gctcctcctt tcagagagtg gttccggggt ggtgtgtgaa
     1441 cttgtgcgcg tgttgtgtga acgaaacaga tgtgtcagcg gtgtcttcat tgtccttccc
     1501 cttccgctgc gccgggtaag gacactctca cccgaacggt ggctactgct ttggaacgcg
     1561 gccggatcgg ttcattgacc agccatttga tgtgcgtgta ccggtcggcc ggaacatgcc
     1621 gtttctcaat tgtcccgtcc gggttctgga agtcgtacat gatgggcggc ggttggaaaa
     1681 acctgcctcc atctatgctg taggtgatcg tcatccctac gcctgcggcg cttccgatga
     1741 cgtactccgt gccgcgcgga atgggatcga cgatttcgac gccgtacgcg ggttccgtgc
     1801 cgttattcac ggcggtaagc gtgtaccgga tgatgtcgcc cggtttcgag atgaggttgc
     1861 ccgcgctatc cctcaccgcc ttgccgatcc gagcatcacc ggcgggaaga acgagctcgt
     1921 cagtgatggt gatggagact ccgttcggcg cgggctgctg accggttgca ggttcgctga
     1981 ccgccagtgc agcacacacg gcgatcagga ggaccagaac ggttccgctt cgtctggtgc
     2041 gcatggtgct acccataaca gactcctttc ctacttcact ttcacgcgga acgaaaacgt
     2101 cccgctcccg cccacaggga gggttcccac tgttacgctc acgttgttcc cggagacggt
     2161 gacctgatcc gcgtcggccg cgtcggtttt cggcacgcca ttcagaacga cgctgttggg
     2221 gacgtagtcc gtttcgaacg gcaccgtatc cgagatgatg aggaaggtcg ccgcgtcggt
     2281 gccgttgttg ccgtacgtga cggtgtaaac gagcgtatct cccggcgcgg cagtggcctt
     2341 gtccaccgtt tttgagcagg ttgctatggg ctgggatgtt accgtgatcg tcgcggtcac
     2401 aagcttcgaa agccccgtca tagcgcccgt gttatatgtt atccagcctt tgttcagaat
     2461 ctccgtctgg ttcgcgatcg ggctcttgac gaccccgcgg aacgtgaacg tcgcggaggc
     2521 ccccgctgcc agcgtcccca cattccactg aagtgccgga ttcgtcgccg ccggcgtcgg
     2581 cgtggcggaa ttgggaacgt agttgaggta cgtggagacc gtgtccttca gcacgacact
     2641 ggtggcggtc ccgccgccgg tgttctgaat cacgatcgta tacagcagtg tgtcagcggg
     2701 cagcgtaagc ccgccgttga ggtcggaaac cgacttcgtt gacgtgttga ggacggcgat
     2761 cgcgctgcac gtattggtga acttggcaat gaggtacgcc gtgccggccg ggttgcttgt
     2821 cgaatacgtg gtgttgccct gtttgtcacg cgcgaccgcg cggatcagga aattgccggc
     2881 ggacatctgc gacacatccc agacataccg gaattgcccg atccccgtgc gggtccccgc
     2941 gccgatacgg gtccacgtct ggccggggtc gttctcaccc cagtcgccgt tgtaatcgta
     3001 gtaatactcg aaccatacgg agtctattgt gtccgtcgtc agcccgccga ccacggtcag
     3061 gacgtccgac accctcgctt tgagcgtatc aggggaaggg cagcccgtct gggtaagctc
     3121 ggcgatgatc ggcgcatcga ttcggccgcc gtacccgttg tagatgtcgc cgcccggcag
     3181 cggaacatcc gcgagagcga agtccccgct gtaaagcagg tccttctggg tgggatcgga
     3241 gttgctcgcg ctcgtggtgg ctgaaatgga gaagaagctt gaaccggtca gcaacgggcc
     3301 tccattggcc ctgccgtcga aggcctcaag cggtacctgc acgtcaatcc agtactcact
     3361 tttctgactg ccgaccggtt tcgttctatc aatctgcacg acccgcgttc tgccgaaatc
     3421 ccagacgtac gcgtcgggat cggtatccca cgttgagctg cttcccgagg cgccgtccac
     3481 tccatcgctc ggtcccgcgc aatcctgtcg ccagacgccg acctggttca tgtcgaactt
     3541 ctggctgttc acgttctcgt aaatgatgac gatgtcgtcc ggttggtttc cggagctggt
     3601 gccgtcgagg aaaacgacga actccttcca cccgtcgccg tcgatgtcca tcagcacgtt
     3661 ccacgtggcg gaggtgaacg gctgcccgct tcctgtcagg gctgtgggtg gcccgccgac
     3721 gcgaaagcgg aagtaaagga tggtgccgtt cccgtagtag taaaacgagg gctggtcgta
     3781 cagcggaccg gaagtgaaat cgttggcgcc ttgcggcgaa gcacctttgg aaccgtcggt
     3841 ttgaccggtc ttgtccgctt ccggcacgcc gaggcgcgta tacgcgctcg tggccaacac
     3901 gctgggccag gttgtggggt acgtgtaccc cgtggggtcg cggtagttct ggagggccgc
     3961 cggtacggcg gaggcagcga cgaggaggac ggcgacaagt gccgggaaaa gcggaaatcg
     4021 cctacgcgtc gagggaacgc gtacggaacg cggaaacagt atctcagacg acggcaatgc
     4081 ctacggccct ccggacattg tctcgcacac gcgttgcaca gacgccactg ctgtggcagc
     4141 gcccgccacg ggagcaggcc tctccggccc ttgtcggagc cggcgaatcg aaagcgttca
     4201 tacctaaatg taccaaaagg tcaacccctc tagtgcaaga gctccgtact tcgttcgcca
     4261 cgcaagacat tgtgaacgcc ggaaaaccaa gcaaattaag taccggaaga acggcactgc
     4321 cttcttgccg ttccagacgg gatttgtggc acgcagccgt ttcgtgaaaa cccccgaagt
     4381 gtctcaaaat gagaaaagtg ttaagaacgt cacacttccg tgagacagaa aattcctcgc
     4441 ggcgccaaac cccgcgggaa acaccggaac ccgttcagtg caaccgggtg cggcaaaaag
     4501 cgcaggaggg ttcgcgtcgt ttccggcttt tgattacctt tacttttctt tgaaccatcc
     4561 agaaccgctc cgtggttccc cgaccgcgct ataccgcggg attcaaccgg cgtcgggtac
     4621 ggtctggctg acgcaaaagg atgacctctt ggttcctcct ggaaggcagt atcatttctc
     4681 ttacctgccg tgcgcaaaac cgtcgtgagg attatgccgt atgacaacac ggaaaacgct
     4741 gaatcacgtg gtggtggcgg taaaacaggt gcccgacacg acgcaggtga aggtggaccc
     4801 ggcaacgggt acgctgatcc gcgaaggcgt gcccttcatc atgaacccgt tcgacaccca
     4861 cgcggtggag gaggcgcttc gcctgaaaga ccgcttcggc tgcctcgtga ccgcggtgac
     4921 aatgggtcca cccaacgcgg cggccaccct gcggaaatgc ctcgcgctgg gcgtggatga
     4981 tgtggtgctg gtgtctgacc gcgtgtttgg aggcgccgat acgctggcga cgagctacgt
     5041 gctgtcggaa gtgatccgcc gggtggagaa ggaacaggga cgcgtggatc tcgtcatctg
     5101 cggcaaacag acgattgacg gcgataccgc gcaggtgggc cccggcatcg ccgtccgcct
     5161 gaactactgc ctgcttacgc tggtggacag gattctggac atcagcggcg cggagcgaac
     5221 catcacggtg cgcaggaaac ttgaaggccg ccgggaggtg gtgcgcgctc tgatgcccgc
     5281 ctgtttgacg ctggtgcgcg aggtcaacga gccccgctac ccgaaagtgc cgaaccgcct
     5341 gatggcggaa acggccgaga ttcccgtgtg ggataaccag ttcctccagc tcagcctctc
     5401 gcgggtgggg ttgaaaggct cgccgacgca cgtgaagaag atttttgcgc cggagcgcgc
     5461 ccgtggtgaa atactggggg atgcggaaaa cgatccggaa ggcgcggtcg ccctcctgat
     5521 aagacgactg attgaaaagg acctgatttc cgtcgggcga taggccgcag gatgttgctg
     5581 ccggccaacc cgtaacaagg agagattccg cggtgcccga gaaaagcagt ctgggtgaac
     5641 ccaaacgtcc gaaacggccc agaggacgcg cgcgtcttct ggagggcaaa tgcatcgcgt
     5701 gcggcgcgct ctgccaggcg gaatgcccgg ccgacgcaat cgagatgaac gaccggggag
     5761 aacccatcat catcctcgag aactgcatcg gctgcaaaaa atgcgtgaaa gtgtgcccgg
     5821 cttccgccct ggagatgttc ttcacgccgg aggaagaagc ggttcttgcc gagcttgcgc
     5881 ggctcggttt cgcgaaagaa gcgcccgaga aggaggggcc ggttcccaaa gtggaccaga
     5941 gctaccgtgg cgtgtgggtg ttcgtggagc agcagaacgg acgcgcggcg gcggtaagct
     6001 gggaactgct cggcgccggc gccaaactcg ccgccgacct cgaggtggac ctctgcgcgt
     6061 atgtgctggg gcatgaaatc ggccatctcg ccgacgaggc gttcgcatac ggcgcgaaga
     6121 aggtctacct cgttgacgat ccgattctga agaactaccg tacggaaccc tacctcaaag
     6181 cgtcgtccga tctcatacgc aaatacaagc cggaagtggt actgatgggc gccacagggc
     6241 tcggaaggga cctcgccgga gcagtcgcca ccgatctgca caccggcctg acggcggact
     6301 gcaccgggct cgccatcgac aaggctggca ggcttctgga acagacgcgg cctgcgttcg
     6361 gcggcaacat catggccacc attctgacgg agcggcaccg cccccagatg gcaaccgttc
     6421 gcccgcacgt catgccaaaa ccggaaccgg tgagcggcgc gcgcggcgca gtcgtcgtgg
     6481 agcctctgga catgtccgag gacgacattc gcgtcaaagt gctcgaagtg cgggaagatg
     6541 agggcgccgc caatcgtgtt gacatcgcgg gcgcggaggt tctggtctcc ggcggccgcg
     6601 gaatgcaggg gccggagaat taccgcctgc ttcaggaact ggcggacgtt ctgggcggaa
     6661 cggtggcctc ctcccgtgcc gccgtcgacg cgggctggat gccctacgag cgacaggtcg
     6721 ggcaaacggg aaaaacggtg cggccgaaga tatacatcgc gtgcggcatc tccggcgcga
     6781 tccagcacct ggtgggcatg caggatgctg acctgatcat cgcgatcaac cgggataagg
     6841 acgccccgat tttcgaagtg gcgcacttcg gcctggtcgg tgatctcttc cagatcgtgc
     6901 ctcttcttac gaagaggttc cgcgaaatgg caggcaaacc gtccgactcc ggcaacgaga
     6961 aaggctgacg agcatgtctc agacagagaa tgtgatcttt gcggttgttc tggcgatagc
     7021 ggtagccgca tttctgcagt cggcctggcg gctggtgtcg ttcatccggc tggggaaacc
     7081 ggacggcagg ttatcgggag aactggtccg gcgtttctgg accatgctgg tgtatgcgtt
     7141 ctggcaacgg cgggtgagga aggaaccgta cggctggaac cactttttcc tgttctgggg
     7201 gttcatcatc ctcttcctgg caaacgggga gttcgtgatt gccgggcttt tcccgtcact
     7261 aacctttgaa ctgctcgggg aacgtatcta cggggtgctc cttttcctgt ttgacgtcgt
     7321 cagcatgatt gtgctggtca acgttgccat cgcgttcttc cggcggctgg tgctgaaacc
     7381 ggcgcacatc gagtataaca gccgggacgg gtatctcatt ctgagcctca tcggggcgct
     7441 gatgctggcg tttttctgcc tgtatggctc ggaattcgcg tccgggacga tgagtgtggc
     7501 gggcgtcatg ccgttcacgg agacgatctt cgcgccgctg atgggcagcc tgttcggcag
     7561 tgcggcgccg attgccgcgc gcgtattctg gtggctgcac gggctgatcg ttctgagctt
     7621 tttgaactac ctgccgtaca gcaagcacat gcacatcctc accgcgatcc cgaactgcta
     7681 cgcgcggtcg tttgaatatc cggcgacggt gccgcgcgag gagttcaaac cgggcgcgca
     7741 gtttggcgtg tcggtggtgg acgagttctc gtggaaagac ctgcttgact tcaccgcatg
     7801 caccgaatgc ggacgctgca accagaactg cccggcgacc agcaccggga agcctctcaa
     7861 cccgcgcctt ctggtgcacg acgggaaggt gaatctgctg acaaacgggc cgaagcttct
     7921 gcaggggaac cgctcggcga atctgctgcc cttgatcggc acaacagacg aacacgaggg
     7981 gactgtgggc gaggacgctc tgtgggcgtg caccacgtgc ggcgcctgta tgacgaactg
     8041 cccggtgttc atcgagcatg ttcccaagat catcaagatg cgccggcatc tggtggagaa
     8101 tgaggcccgc ttcccgcacg aattgagtgt ctttttcgag tccgtcgagc agcgatcgaa
     8161 tccttggggc atcacgccgg gagaccgcgc gaaatgggcg cgtgatctgt cggtgccgct
     8221 gatgtcggac gcaaaatgcg acacgctgtt tttcgtggga tgcgccgggg cgttcgacgc
     8281 gcggaacaag aaggtggcga ctgcggtggt gcgcgcgctt aaggcggcag gcgttgagtt
     8341 tgcgatcctc gggacggagg aaaaatgctg cggggattcc gtccgccggc ttggcaacga
     8401 gtatctgttt gaacggatgg cgaaggcgaa cgtcgagctg ttcaagaaat acgacgtaag
     8461 aagaatcata acctattgcc cgcattgcta cactacactg aagcacgact atcggcagtt
     8521 cgggatggag acgcaggttg tgcaccactc cgaattcctg catgaactgc ttgagacggg
     8581 gcgcctgaag ctggacggga acaaaccggc cgtggacggg aaggttctgt accacgattc
     8641 gtgttacctc ggacggtaca acgggttgta cgaggagccg agggaagtgg tgcagcgggt
     8701 gacgggacgg gcgccggtgg agatggaccg ccggctagaa aagagcttct gctgcggcgc
     8761 gggcggggga aggatgtgga tggaagagga gccggaacac cgcatcaacg cgaaccgggt
     8821 gcgggaagcg ctggggaaag acgcggatgt gattgccgtg gcgtgcccct attgcatgac
     8881 gatgttcgac gacggcatca aagatgagcg cgcggagggg cgcgtgaagg tgatggatat
     8941 tgcggagctg gtggcggcgc ggctgccggg gtgatgaggg gggcgtgcgg tgcctctccc
     9001 cgatgagtta cgagcttttc gtgcgtctgc cacgactgca gcgtcttttc ctttggagtt
     9061 cctgagtttg atcgcggcga gcggcagctt ctgtgactga ttcgatgcag ctcgtcaggc
     9121 ggagttcatt cgccggcgct caatcagggc aatcgtggga tcgggacggg aataatcaga
     9181 aacatgacag aacaagacaa gaacaggacg gaataagaca agtgcttcat ttacagcctc
     9241 ttccaccttg tacccctgcc atgcctggga caaactgcca agccagacgc cccgcgacgc
     9301 gcccttgccg gaagaggtac gtggtgcaca ggttacccga gaaccgccga gc
//
LOCUS       DAOSOO010000119         9280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7376_length_9280_cov_5.376260, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000119 DAOSOO010000000
VERSION     DAOSOO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7376_length_9280_cov_5.376260"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLT86_09245"
     CDS             <1..455
                     /locus_tag="PLT86_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT36837.1"
                     /translation="KLIGFGFRAIVLHAGHYPWQGVLDKVLSPVRDEHPEVLFIWGTE
                     CTIGGEGLRDVPGDHAARWETSYGLALLPDLVDTGALREGRGLEAWPNATPPPEERRY
                     RGVVFDTQHPLFAQLGEDARKASAEEAEFYLSRIAEAITQRVRNHLER"
     gene            480..3377
                     /locus_tag="PLT86_09250"
     CDS             480..3377
                     /locus_tag="PLT86_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36838.1"
                     /translation="MRPPEDVYQGELVRYPGPWGFNIPRSHIILVSDEQLEQLQDPDA
                     LVDLSLSPTKDLRSLRQVCEQAAARGVRTLMFAFDHFWNQYREGQGNKPRELLPDTDE
                     YISRIAKVSHFVQQYGIGLELSLLSPLELGKGFRNATGETGKWMHVREGIRDPSTGAF
                     FVQLWRQKRWGNNKGAFDVELENVRAFAFRERQIGGTHLFSVVRDEIREITDCVEMDP
                     GCSGMYSAGDYLAERVAVRGNGMADVGDLDRVAVVLTYKTPEMDYFSPSATAFLQDLL
                     ERYRKAGVKLNALYADEMHIQQDWGYFSHHDHGQLALRYVSPHLERRFSELFGREYAD
                     FAPWMVYFCSGQHDFLPTTGAASFCQHVLGSTQDEISATFLLRQRYYHVLEGTVVDLM
                     TDAKHYAEQLAGHTLPARAHVTWAESPTIDDSEPSPLPHYTQLYEYTQSFRWSNTVHQ
                     AASACQDYFRWNDFLTGGGNDHAEGGYADRNYFALALACSTGNLNEVPYAYAAHWGMP
                     KEIAQRRSALVDVFGACASPTFQAVEDSQHRTSNVLMLYPIDLVSVDERFGSWMVQYG
                     YADYITAEKLCELGRVTAAGEIAVKDRRYRTLVALYEPFPSEQLMFLMERLVNQGGRV
                     VWSGTPPFLHAEGTPVRQAWERLFGVTCEHEGAPTGVPVPGRTARFCGPLDGVPAQTI
                     LTNTVVDHIYPFVVSGGSEVSAKVGKWVVGAVRSAGTRGGKAVALGFRPRDDQSQSLG
                     DEVRTWFEILVKLGSYPPSGLFGDVNDNPEYLSRTKPWLAQAFPNGAIAVAPHFRTYE
                     EGWHGGFHRDDEADRKWLNDHPLPSNEICLEQELIHGVRLTYRGEGAVALRLNGDGDL
                     IAFAGSACSGVTVNDRSFVFSDHPVRHLGWSPVPPEQRVPGGAVFRVWIDSDRDVSVP
                     VPAGLPPLRWWCQGPKPGCRGVPVTASKTDSAVHFGVHEGICRTWIFGVPE"
     gene            3487..6090
                     /gene="glgP"
                     /locus_tag="PLT86_09255"
     CDS             3487..6090
                     /gene="glgP"
                     /locus_tag="PLT86_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOT36839.1"
                     /translation="MKPVGSFTIVPSLPERIARLRELALNLWWCWEHEAIDLLRRLDY
                     DLWDSPDVYHNPVKMLNIVPQKRLQELSEDEGFLAQYDRVVKWMDAYMEADVKGATWF
                     RRTYPDAAKSCYAYFSAEFGLNECLPIYSGGLGLLAGDHLKSASDLGIPLVGVGLLYQ
                     QGYFQQYLNNDGWQQEGYPINDFYSMPLQKAVRNDGTQAFVEVSLPGRAVRAAIWKVQ
                     VGRVPVYLLDTNIRENSREDRDITDQLYGGNQEMRIKQEILLGVGGKRALAELGIRPT
                     VFHMNEGHSAFLAVERLREMVQEEKKPFAEALEITEASTVFTTHTPVPAGNDRFAPEL
                     IDRYLYGYYAALGLSREQFLALGQENPGNVHSEFCMTALAIRLAAHTNGVSRLHGRVA
                     RGMWKHLWPGLPVEEVPIASITNGVHARSWISNDMGWLFNRYLGTRWMERPADQTIWQ
                     AISRIPDEELWRTHERRRERLVAFARRRLRSQLERRGATRREIQSAGEVLNPDVLTVG
                     FARRFATYKRADLLLRDPDRLIALLTNKERPVQIIFAGKAHPADSPGKELIRKIIHFI
                     RLNDELRRHVAFIEDYDINVARYLVQGVDVWLNTPRRPLEASGTSGMKAAANGVLNVS
                     ILDGWWDEAVRDNPGVDVGWAIGNGEEYRESESEYQDEIESRALFNLLEKEVVPLFYD
                     RSSDGVPRGWTERMKASMRTLCPVFNTNRMVCEYTGRFYRDAENRWRDITADECKKAR
                     DLAAWKTRVFREWHNVRVTRIESDRVNELPVGKMLHVLATVSLGNLLPADVSVQVYHG
                     RLDSHGEIVEGTATNMSFVSQGSDAVFEGDVPAIGSGQHGFAIRVVPFHPDLSNPFAT
                     GLITWSPESLG"
     gene            6116..7339
                     /gene="trpB"
                     /locus_tag="PLT86_09260"
     CDS             6116..7339
                     /gene="trpB"
                     /locus_tag="PLT86_09260"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT36840.1"
                     /translation="MTNVPASTYSVPDNTGHFGIYGGRFASETLMPLLSDLAREYEAA
                     KADAGFQEEFSGLLRNYVGRPTPISFAKRLSDHIGGARIFLKREDLAHTGAHKINNTI
                     GQVLLARRMNKRRIIAETGAGQHGVATATVSALFGLECEVYMGAEDMRRQRLNVFRMR
                     LLGAKVTAVHSGAKTLKDAINEAMRDWIARVDDTYYIFGSVLGPHPYPMIVRDFQSVI
                     GKEARAQMQEEIGRLPDVCLACVGGGSNAMGLFHAFLGDDNVRLIGVEAGGRGTRLGE
                     HAARFRGGRLGVLQGSKSYVLQNEDGQITPTHSISAGLDYASVGPEHSYLRDLGRTEY
                     TVASDSQALEAFHLLSRLEGIMPALESAHAVAEAISLAKTMAKDQVILVNLSGRGDKD
                     VHTVAETMGIDIGAI"
     gene            complement(7343..>9280)
                     /locus_tag="PLT86_09265"
     CDS             complement(7343..>9280)
                     /locus_tag="PLT86_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36841.1"
                     /translation="NLYLATLPTDSTEEVSYERLTNVLGGAFRPAVAPDGRWLAYVGY
                     HANNFSLYILPLAADGQAALRPPFAGDAVPRSYQHIAKSPPASQLFHVGQTTGMRTLV
                     GWVPVLRFGPSVIGDRFSVNHVGLGLLAGIEDQMGARYYFGSGEISKNIGKREPASTG
                     VFLYGDQGLRPLLTTESGLSPRLYGYGARQEICAVTERARTTRASAPGAVLGANNETI
                     PLNPLSRVELSDSVVGWDNYRYLYGGLGIRAGVGSRQTAAFELGWQNYYVDERLDRRI
                     DNYSVFLDNSSPPQDVTALFPWGNYGYPNPGTPLIWDQRPQFSVAYFRDRTASFRWAY
                     AKITPTVDDLVNPTAGRVITASYSFHRVSVMDSLAGTNYDANYNQIDPTRPLYSQVDR
                     HFSANELVLSYEEYIRLPGVLNRTTLALQGVAAYFDRALKYYDPNDEGSNFLEGWAYW
                     PLRYRLGGGGTLRGYPYFSHEGSKMAFFRASYVFPLLQHHRKQFLSFFHDRTYGALFV
                     ETGSTWNWTRLKDAWIARDDFLWDLGAELRISGLMFHYMPVVSYVAVARRMKDVPYPF
                     VDYERTAEGAILFRQPDRYRVYIGAALALGGGGGAAPHAEPRFTRTPQVLMLSNPARQ
                     PANGAWESPGTTAEKLPLLFR"
BASE COUNT         1938 a         2566 c         2874 g         1902 t
ORIGIN      
        1 gaaagctgat cggcttcggt ttccgggcga tcgttctcca tgcgggacat tatccctggc
       61 agggagttct ggacaaggtg ttgtccccgg tccgtgacga gcatcctgag gtgcttttca
      121 tctggggaac cgaatgcacc ataggaggcg aggggctgcg cgacgttccg ggtgaccatg
      181 ccgcacggtg ggagacgtcc tacgggctcg ctcttctccc ggaccttgtg gacacagggg
      241 cgctgagaga aggccgcggg cttgaggcgt ggccgaatgc gacaccgcct ccggaggaaa
      301 gacggtacag aggggtggtt ttcgacacgc agcatccgct tttcgctcaa ttgggagaag
      361 acgctcgcaa ggcttccgcg gaagaggctg aattctatct ttcgcgaata gccgaggcga
      421 tcacgcaaag ggttcgaaac catctggaac gttagattcc gattttcggg gaggtgctcg
      481 tgaggccgcc ggaagacgtg tatcagggcg aattggtccg gtaccccggg ccgtggggat
      541 tcaacatccc tcgctcgcac ataattctgg taagtgatga gcagttggaa caacttcagg
      601 atcctgatgc gctggtagat ctgagcctga gtccgacgaa ggatctgcgc tcgcttcgtc
      661 aggtgtgtga gcaagccgcg gcgaggggag tgcgaacgtt aatgttcgcg tttgaccatt
      721 tctggaacca gtaccgggaa ggtcagggga acaaaccgcg cgaattgctc ccggacacag
      781 acgagtacat ctcccggatc gcgaaagtga gccatttcgt ccagcagtac ggaatcggcc
      841 tcgaactgag cctgctgagt cccttggaac tgggaaaagg gtttcggaat gcaaccggcg
      901 aaaccgggaa gtggatgcat gtccgcgagg ggattcgcga tccttccacg ggtgcctttt
      961 tcgttcaact gtggcgtcag aaacggtggg gtaacaacaa gggcgcgttc gacgtggagt
     1021 tggaaaacgt ccgcgccttt gcgtttcggg agagacagat cgggggcacg catctgttct
     1081 ccgttgttcg cgacgaaatt cgagaaataa ctgattgtgt ggagatggat cccggctgca
     1141 gcggtatgta ctcagcgggt gattacctcg cagaacgtgt ggccgtacgc ggaaacggga
     1201 tggcggatgt gggagatctc gaccgcgtgg cggtagttct cacctacaaa acacccgaga
     1261 tggattactt cagcccgtcc gctacggcgt ttctgcaaga tctcctcgaa cggtaccgga
     1321 aggcgggcgt caaactgaac gctctttatg cggacgaaat gcatattcag caggactggg
     1381 gatacttcag ccaccacgac cacggtcagt tggctctgcg atacgtgagc ccgcacctgg
     1441 agcgtcgttt ctccgagctg ttcggccgcg agtatgccga tttcgccccg tggatggtgt
     1501 acttctgttc cggtcagcac gatttccttc ccaccaccgg tgcggcatca ttctgtcagc
     1561 acgttctcgg gagtacgcag gacgagattt ccgccacgtt tctgctccgg cagcgttact
     1621 atcacgtgct ggagggaacg gttgtagatc tcatgactga cgcgaagcat tacgcggaac
     1681 aactggccgg ccatacgctc ccggcacgcg cgcatgtaac ctgggcggag agccccacga
     1741 ttgacgactc ggaaccgagc ccgctgccgc actacacgca gttgtatgaa tacacccagt
     1801 cgttccggtg gtcgaacact gttcatcaag ctgcgtcggc atgccaggac tatttccgat
     1861 ggaatgactt ccttaccggt ggaggcaacg atcacgccga aggtggctac gcagaccgca
     1921 actactttgc gcttgcactg gcgtgctcga ctggaaacct gaatgaggtg ccatatgcgt
     1981 acgcggcgca ctggggtatg ccaaaagaaa tcgcccagcg ccgttccgcg cttgtagacg
     2041 tgttcggtgc gtgcgcatcg cccacctttc aggcagttga agactctcag caccggacaa
     2101 gcaacgtcct gatgctctac cccatagatc tcgtgtcggt ggatgaacgg ttcggaagct
     2161 ggatggtgca atatgggtac gcggattata tcactgcgga aaagctctgc gaactgggac
     2221 gagtgacggc tgcaggggag attgccgtca aagatcggcg ctacaggacg ctcgtggctc
     2281 tctacgagcc atttccttcc gagcagttga tgttccttat ggagcgcctt gtcaaccaag
     2341 gtgggcgtgt agtctggtcc ggcacgccgc cgttcctcca tgctgaggga actccggtcc
     2401 ggcaggcatg ggaacgtctg tttggagtta cgtgcgagca cgaaggcgcc ccgacgggtg
     2461 tgccggttcc cggcagaacc gcgagattct gcggcccgct tgacggcgtt cccgcgcaga
     2521 ccatcctcac caacaccgtg gttgatcaca tctatccgtt tgtcgtcagc ggaggatcgg
     2581 aggtgtctgc caaagtcggg aaatgggtgg taggggccgt tcgttctgca ggaacgcgcg
     2641 ggggcaaagc ggtggctctg gggttccgtc cgcgggacga tcaatcccag agcctcgggg
     2701 atgaggttcg gacgtggttc gagatccttg tgaagcttgg gtcctatccc ccgagcgggt
     2761 tgttcggaga cgtcaacgat aatcccgagt acctttctcg aacgaagccg tggttagcgc
     2821 aggcctttcc caacggagcg atcgcggtgg caccccattt ccggacttac gaagagggat
     2881 ggcacggtgg gtttcaccgt gatgatgagg ccgacaggaa atggctgaac gatcatccgt
     2941 tgccatcgaa cgagatctgt ctggaacagg aactgataca cggcgtgcgg ctgacgtacc
     3001 gaggcgaagg cgcggtggct ctccggctca acggtgacgg tgatctcata gcttttgccg
     3061 gctccgcatg ctccggggta accgtcaacg atcgttcctt tgttttctcc gatcaccctg
     3121 ttcgtcatct gggttggtcg cctgttccgc ccgaacagcg ggtacccgga ggagcggttt
     3181 tccgggtgtg gattgattct gaccgcgatg tctccgtacc cgttccagca ggacttccgc
     3241 ctttgaggtg gtggtgtcag gggccgaaac ctggatgcag gggtgttccg gtgactgcgt
     3301 caaagacaga tagcgcggtc cattttggag tgcatgaggg aatctgccga acgtggatct
     3361 tcggcgtccc tgaataacgc gcgaagatga ccgcttaggc cgctgatcga ggtccgccgc
     3421 acatgctctc tggaaggctc tggttcgtgt tgtatcgcgc aaaacgcaca tctggaaggt
     3481 agttccatga aacctgtcgg tagcttcacg atagtgccct cacttcccga gcgcattgcg
     3541 cggttgcgtg aacttgcgct caacctctgg tggtgctggg aacacgaagc catagacctc
     3601 ctgaggcgtc ttgattacga cttgtgggat tcgccggacg tctatcataa tcctgtgaaa
     3661 atgctgaata tcgtgccgca gaaaaggctt caggagcttt cggaagacga ggggtttctt
     3721 gcgcagtatg accgcgtggt caagtggatg gacgcctaca tggaagcgga tgtgaaaggt
     3781 gcgacgtggt ttcggcgaac ctatcccgac gcggccaagt cctgttacgc ctatttttcc
     3841 gcggaattcg gactgaacga gtgtctgccc atctactccg gcggcctggg cctcctggcg
     3901 ggtgaccatc tgaaatcggc aagcgacctc ggcattcccc tggtcggtgt ggggctgctt
     3961 taccagcagg ggtatttcca gcaatacctg aacaacgacg ggtggcaaca agagggctac
     4021 ccgatcaacg acttttactc gatgccgctg cagaaagccg tcagaaatga cggaactcaa
     4081 gctttcgtgg aggtttcact cccgggcagg gcggtgagag cggcaatatg gaaagtccag
     4141 gtcggacgtg tccccgtcta ccttctggat acgaacatca gggaaaacag ccgggaggac
     4201 agagacatca cggatcagct ctacggcgga aatcaggaaa tgcgcatcaa acaggaaatt
     4261 ttgcttggcg tgggagggaa gcgcgcgctt gcagaacttg gcatccgccc caccgtgttc
     4321 cacatgaacg aggggcactc ggcgtttctc gcggtggagc gtctgcggga gatggttcag
     4381 gaggagaaaa agccgttcgc ggaagcgctg gaaatcaccg aagcgagtac cgtgttcacc
     4441 actcacacgc cggttcccgc cggcaatgat cgattcgctc ccgaactgat tgaccgatac
     4501 ctgtatggtt attacgcggc gctggggctt tcccgcgagc agttcctcgc attggggcag
     4561 gagaaccccg ggaatgtgca cagtgagttt tgcatgaccg cgctggcgat ccgcctcgcg
     4621 gcccacacga atggcgtcag caggttgcac gggcgcgtcg cgcggggaat gtggaaacac
     4681 ctctggcctg gcctgcccgt ggaggaggta cccatcgcct ccattaccaa tggcgtgcac
     4741 gcgcggtcgt ggatctctaa cgatatggga tggttgttca accgttacct cggtacccgt
     4801 tggatggagc gaccggcgga tcagacgatc tggcaggcga tttcccgcat cccggacgaa
     4861 gaactctggc gtacccacga acggaggcgt gagcgtctcg tcgcatttgc gagacggcgc
     4921 cttcggagcc agttggagcg acggggcgcg acccggcgcg aaattcagtc tgcaggcgag
     4981 gtacttaacc ctgatgtact gaccgtaggc ttcgcacgtc gctttgccac ttacaagcga
     5041 gcagatctcc ttttgaggga tccagatcgc ctgatagcgc tcctgacgaa caaggaacgc
     5101 cccgtgcaga tcattttcgc gggaaaggcc caccccgctg attctccggg gaaggagttg
     5161 atacggaaaa tcatccattt catcaggctc aatgacgagt tgcggcgtca cgttgccttc
     5221 atcgaagact atgacatcaa cgttgcccgt tatctcgttc aaggtgtgga cgtctggctg
     5281 aacacgccgc ggcgcccgct ggaagccagc ggaaccagtg gcatgaaggc tgcggctaac
     5341 ggtgtgctga atgtaagtat tctcgatgga tggtgggacg aagcagtcag ggataatccg
     5401 ggcgtggacg tcgggtgggc aataggaaac ggagaggaat acagggaaag cgaatcggag
     5461 tatcaggacg agatagaatc gcgcgcgttg ttcaatctgc tggaaaaaga agttgttccg
     5521 cttttctacg accgctcgtc agacggggtg ccgcgcggat ggacggagcg catgaaggcg
     5581 agcatgcgca cactctgtcc tgttttcaac accaatcgca tggtatgcga gtacacgggc
     5641 cggttctacc gggatgccga aaaccggtgg cgtgacatta ctgccgacga atgcaaaaag
     5701 gcacgtgatc tggctgcatg gaaaacccgg gtattccgcg aatggcacaa cgtacgtgtg
     5761 acgcggatcg aaagcgatcg tgtaaacgag ctgcctgtgg gcaagatgtt gcacgttctg
     5821 gcgacggtca gcctcggaaa tcttcttcct gcagatgttt cggtgcaggt gtaccacggt
     5881 agacttgact cccacggcga aattgtcgag ggcaccgcca caaacatgtc gttcgtatcc
     5941 cagggcagcg acgcggtttt cgaaggggac gttccggcta tcgggtcggg gcaacatgga
     6001 tttgcgattc gcgtggtgcc gttccatccc gacctctcca acccgttcgc caccggactc
     6061 atcacgtggt cgccggaatc gcttggctga acccatccgc aggagcttct ctcacgtgac
     6121 caatgtacca gcttcgacct acagcgtgcc ggacaacacg ggccattttg gcatctacgg
     6181 agggcgcttt gcgagcgaaa ccttaatgcc cttgctttcc gacctcgcgc gggaatacga
     6241 agcagcgaaa gcggatgccg ggttccagga ggagttttcg ggactgctcc gaaactacgt
     6301 ggggcgcccc actccaattt cgtttgccaa aaggctctcg gaccatattg gaggagcgcg
     6361 gatattcctc aaacgcgagg acctggcgca tacgggtgcg cacaaaatca acaacacgat
     6421 cggccaggtg cttctggcgc gccgcatgaa caagcggcgg ataatcgcgg aaacgggcgc
     6481 cggacagcac ggtgttgcaa cggcaaccgt cagtgcgctg ttcgggcttg aatgcgaagt
     6541 atacatgggt gccgaagaca tgcgccggca aaggctcaac gtctttcgta tgcgtttgct
     6601 cggggcgaaa gtgacagcgg tacattcagg cgcaaaaacc ctgaaagatg cgattaacga
     6661 ggcgatgcga gactggatcg cccgtgtcga tgacacgtac tacatttttg gctccgtgct
     6721 ggggccgcac ccgtatccga tgatagttcg tgatttccaa tccgtcatcg gaaaggaagc
     6781 gcgggcgcag atgcaggagg agatcggtcg cctcccggac gtctgcttgg cgtgcgtagg
     6841 gggcggttcg aacgcgatgg gcctgtttca cgcgttcctg ggcgacgaca acgtgcgtct
     6901 gattggtgtg gaagcgggag gcaggggaac ccggctcggg gaacatgcgg cgagattcag
     6961 aggcgggcga cttggtgtgc tgcaagggtc gaagagttat gtccttcaga atgaagacgg
     7021 gcagattact cccacgcact ccatttccgc cggcctcgac tatgccagcg tcggtcccga
     7081 gcactcctat cttcgtgacc tcggccgcac ggaatatacc gtggcgagtg attctcaagc
     7141 gctggaagca ttccacctgt taagccgtct tgagggtatc atgccggcgc tggaaagtgc
     7201 tcacgcagta gcggaggcaa tttcgctcgc gaaaaccatg gcgaaggacc aggtgattct
     7261 cgtgaaccta agcggccgcg gggataagga cgtacatacg gtcgcggaga ccatgggcat
     7321 cgatatcggg gcgatatgag ggtcagcgga aaagaagggg aagtttttcc gcagtcgtgc
     7381 cggggctttc ccacgctcca ttggccggtt ggcgcgccgg gttggagagc atgagaacct
     7441 gcggcgtgcg tgtgaaacgt ggttccgcgt ggggggccgc tcccccgccg ccgcccagcg
     7501 caagcgccgc accgatatag acgcggtaac gatcaggttg ccggaaaaga atcgctcctt
     7561 ccgccgttcg ctcataatcc acgaacgggt aaggaacgtc cttcatgcgc cgcgctacag
     7621 ccacatagct caccacaggc atgtagtgga acatcaggcc cgaaattctc agttctgcgc
     7681 ccaggtccca gaggaagtcg tcccgcgcga tccacgcatc cttcagcctc gtccagttcc
     7741 atgtgcttcc tgtttccacg aacaacgccc cgtacgttct gtcgtggaaa aatgacagaa
     7801 actgcttgcg atggtgctgc aatagcggga agacataact cgcgcggaag aaggccattt
     7861 tgcttccttc gtgcgagaaa tagggatatc cgcggagcgt gccgccgcca cccagacggt
     7921 accgcaatgg ccagtacgcc catccctcca ggaaattgga cccttcgtcg ttagggtcat
     7981 agtacttgag cgcacggtcg aaataagcgg cgactccctg taacgcgagg gtcgtgcggt
     8041 tcagaactcc gggcaaccgt atgtattcct cgtagctcaa caccagctcg ttcgcggaaa
     8101 aatgccggtc aacctgcgaa tacagcggcc ttgtcggatc gatttgattg tagttggcgt
     8161 cgtagttggt ccccgccagc gaatccatca cgcttacgcg gtggaacgag tacgatgcag
     8221 tgataacccg cccggccgtg ggatttacca ggtcatctac ggtcggggtg atcttcgcgt
     8281 aagcccagcg gaaagaagca gttctgtctc ggaaatacgc aacagaaaat tgcgggcgct
     8341 ggtcccagat caggggagtg ccggggtttg gatacccgta gtttccccac ggaaacagtg
     8401 cggttacgtc ctggggcgga ctgctattgt cgagaaagac gctgtaattg tcgatcctgc
     8461 gatccagtcg ctcgtccacg tagtaattct gccaccccag ttcaaacgcc gcggtctggc
     8521 ggctcccgac acccgcgcga atccccagtc ctccgtagag ataccggtaa ttatcccacc
     8581 cgaccacgga atcgctcaat tccactcgag aaagcgggtt caacgggatg gtttcgttgt
     8641 tggctccaag aacagcaccg ggagccgacg cgcgggtggt cctggcccgc tctgtcaccg
     8701 cgcagatttc ctgacgtgcg ccgtacccgt agagacgcgg cgaaaggcca gattctgttg
     8761 tcagcagagg acgcaatccc tggtcgccgt acagaaacac cccggtgctt gccggctcgc
     8821 gcttcccgat gtttttcgaa atctccccgc tgccgaaata ataccgcgca cccatctgat
     8881 cttcgattcc agcgaggagc ccaagtccta catggttcac ggaaaaccga tcgccgatga
     8941 cgcttggccc gaagcgaagc accgggaccc agcccaccag cgtccgcatt cccgttgtct
     9001 gaccaacgtg gaaaagctgc gaagcgggag gagatttcgc gatatgctgg taggaacgcg
     9061 ggaccgcgtc acctgcaaat ggcggcctta acgcggcttg tccatctgcc gccaatggca
     9121 ggatgtaaag gctgaagttg ttcgcgtggt aacccacgta cgccagccat ctgccgtccg
     9181 gagcaaccgc cggccgaaac gcacctccga gaacgttcgt caaccgttcg tatgatactt
     9241 cctctgtgga atctgtcggc aacgttgcca ggtagaggtt
//
LOCUS       DAOSOO010000120         9256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7404_length_9256_cov_5.410934, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000120 DAOSOO010000000
VERSION     DAOSOO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7404_length_9256_cov_5.410934"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLT86_09270"
     CDS             complement(<1..1207)
                     /locus_tag="PLT86_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT36842.1"
                     /translation="MSGTPSEFFVAPHGNDANEGSACAPFATLERARDQIRALAASAG
                     LPEGGVTVWIRGGVYQRQKAFELTEEDSGKESSPIAYRAWPGESARIIGGVMIPHFSR
                     VDDASVRKRLQPSVRDRVLSADLSEYGVTDCGTLTSRGFARPVLPAHAELFIEGEPMT
                     LAQWPQSGEFLKIAGFDKPLTDEWGTTTGDLTGGFTYQGDRPLAWEPDDDIWVHGYWS
                     YDWANSYERVRHIDPHTRTVTTHPPHGNYSYRVGQRFYFLNVLEELDAPGEYYVDRRK
                     GRLYLLPPNDQEVPSDAILSVLEAPLVAMQIVSHVSFDGLTLECSRGDGLVATDCERV
                     SVRSCTIKNLGNRGIVIRGGRNVAVAGCTVFNNGDGGLDVEGGDRQTLEPADHVVVNN
                     HIHHIARWSR"
     gene            complement(1475..1906)
                     /locus_tag="PLT86_09275"
     CDS             complement(1475..1906)
                     /locus_tag="PLT86_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36843.1"
                     /translation="MAGSEILKRHWRDFDNLRRAFRNHEIVIGLDRRFVLFWVLLSGA
                     ECRFAARATGWMLVVSILVAVAGASLAAWNSLYILAALCALCAVFLWRFLVEFAVKAA
                     RKAVLNDERLFRKWFSELRLSVFVKSTGEYVWNDAAQRARE"
     gene            complement(1893..2765)
                     /locus_tag="PLT86_09280"
     CDS             complement(1893..2765)
                     /locus_tag="PLT86_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36844.1"
                     /translation="MSPPAPIFSDRITAIAAELANLGKRPISAATFQEAKRLHQQLDA
                     LLREFVPCDRCGNFFPNIAQLTLDGRSANMCASCAITALQRGTIDTPVTEQARITHDE
                     PKNSPEKSTPVLQDAASVAPEMVSPARPPVREKTSQTETPRRALPVRELRARVEDEDE
                     PEVRLERSDEPQDGEDEEEKDDSPAVVSARPRVSRAPRLVARGAPNGADQMKASHGEV
                     ARYLNCPTRDVRQIAKLIEEISPPMDIPKTTRYVLAELRNARSKIPPDTVSKVVHFLK
                     DGFPAGGDGAHGGQ"
     gene            complement(3040..3972)
                     /locus_tag="PLT86_09285"
     CDS             complement(3040..3972)
                     /locus_tag="PLT86_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOT36845.1"
                     /translation="MRTTTVAKTNRTARDRTTANPDSVRSDLGTALENLCNTYDESAL
                     DPDPLVVVREFSDPRDQEIAAFFSAMFAYGNARIIVRNLRDLFRRMPGGPYAFVTASH
                     HSDDARCLTGWRHRLHTGEDVAIMSSILAQVLRKHGSLERAFCRGLPKDAPDVGVALH
                     AFVSFLREQGGRRIEQRRFFRHLLPSPADGSACKRMNLFLRWVVRRASPDLGLWRTVL
                     PSLLVLPLDTHVARICRQIGLTRRSTIDWKMALEVTRKLRRFDPADPIRYDYALSRLG
                     ILGDCPLKMDISNCSRCSLQTYCVTFSERGSSQS"
     gene            complement(4030..6894)
                     /locus_tag="PLT86_09290"
     CDS             complement(4030..6894)
                     /locus_tag="PLT86_09290"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOT36846.1"
                     /translation="MAIVEQKTRDFSGCARHVASDPLTRAQYSTAACMYQITPSTVVF
                     PESEAEVVAVVRRAAETGIPVTARGGGSSLVGQALGRGVILDFSRFMRAVLELDENRR
                     RVRVQPGITLGVLNRHLAARGLMFPPDPSSGDYATIGGMIACNSSGARSVKYGSTIDY
                     VAAVRCVLADGSVVEFRGSEAVPISPQTSLLTRLVDEIPAILRQHDSHIREMFPKCAK
                     NSSGYQLAKALRPDGVDLASLIVGSEGTLGIVTEAELEIVPKPLASSMVLVNFASVEA
                     AGEAVHTILPLSPSAVEIMDQTALDMIRAHRPELTAFLPTDAVAQLFVEFDGDNSETT
                     ESACRMAVERSHDLATGHVFAFDAATHSALMNVRKATLPILYTLPGKKRPVAFVEDVI
                     VPPESLGTFIGDLKAIFQGFDVEAAIYGHAGQGNLHVRPLLDLTDARDIAKLEPLASA
                     VFGAVCRFGGSMSGEHGDGRARAPFLRQMYGELSDVFVDVKELFDPSNILNPDVKVTR
                     APHALDSDLRLGSGHTLQPLGTPLPVAPDEITDIISRCHGCGTCRIPEPPVTMCPVYK
                     ALGDEAATPRAKANLLRLFASGELPESFRKTKDWKRILDTCIGCRMCSFECPSGVDIG
                     RIMLIAKGQYCEHKGRSIAEIAMGASDFLSSAGRFFSPLSNVVARQPLFGAALDLFSG
                     IDHRRRLPRFAGKTFSSWHHTHGKNEQSAGEEAVVYFPDQWAQYHDPDVGKALHAVLN
                     RLGIRMIVPETLPCGIAAAANGGLTRVRRLASHNVDALRPYAEAGLRIIATEPTATLF
                     LKDEYRDILGSSAAELVAGCMQDASQFIEQRLRTGTDLPLRALDFSVAYHAPCHLKAM
                     RVGLPAVDILRRIPSLRVQILDEGCCGMAGTYGMKRAHFETSMAIGKRLLDHLADPSI
                     NYGVTDCSTCKLQMEFGTRKTTLHPLKLLACSMGAEVPLR"
     gene            7110..8369
                     /locus_tag="PLT86_09295"
     CDS             7110..8369
                     /locus_tag="PLT86_09295"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT36847.1"
                     /translation="MNSGILAVILGGGQGARLYPLTKVRSKPAVPIGGKYRLIDIPIS
                     NCLHSGIDTVYVLTQFNSVSLHRHIHRTYRFDVFGRGFVEILAAEQTMENTGWFQGTA
                     DAVRRSLRYIDLPDYKHILILSGDHLYRMDYREFVATHLRSNNDVTVSVKPVGLEETS
                     GFGIVRMDESREIIEFLEKPTEPADLERLRLPDAPPGRNFLASMGIYVFRREVLVDLL
                     NRGTEEDFGRQVIPRAIAETRVGAHLFDGYWQDIGTIGSFHRANIDLAAELPPFNFYE
                     PGAPIYTHPRFLPSSKINNAHVQQSMLSEGSIVMEADIQQSIIGIRSIIQRHAVIRKS
                     VIMGADFYETDRDRAHNRSIGRPDVGVGEKCLIENAIVDKNARIGDGVQIINAAKINN
                     ARHADYVIADGIVVIPKDAVIPPGTVI"
     gene            8366..>9256
                     /locus_tag="PLT86_09300"
     CDS             8366..>9256
                     /locus_tag="PLT86_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT36848.1"
                     /translation="MKPPIDRAWCGMTGLPLVLENVSKSFGPTRAVDNLSLALRAGSV
                     CGLLGPNGSGKTTTIRMIMGILLPDSGDLRLWGAPAEGAVRKHVGYLPEERGLYATMT
                     VFDHLIFFAELHDLSRRDAKDAVAEWSERFATNGESSPKSWWGRRVQELSKGQQQTVQ
                     LIGTLLHRPGLVILDEPFSGLDPVHTGHLRTIIRYLKESGVAVLLSTHRMEQAERLCD
                     DICLIHHGKAVLSGALREVKSASTSRLVELRMDRVPDFLEPDDLIAAVEIVSDGARIR
                     LRQGADAQMVLDRAIRRGRVL"
BASE COUNT         1884 a         2720 c         2749 g         1903 t
ORIGIN      
        1 aacgggacca gcgtgcgata tggtggatgt ggttgttgac caccacgtgg tctgccggtt
       61 caagagtctg ccgatcgcct ccttcgacgt caaggccgcc gtcgccgtta ttgaacaccg
      121 tgcatccggc taccgccacg ttccttccac cccgtatcac aatcccccgg tttccgaggt
      181 tcttgatggt gcatgatctt actgatacgc gctcacaatc cgtggcgacc aagccgtctc
      241 cccgcgagca ttcaagcgtg agaccgtcga acgagacgtg agagacaatc tgcatcgcca
      301 caagaggagc ttcgagaaca gaaagaatcg catcgcttgg tacctcttgg tcgtttgggg
      361 gaagcagata gagtctccct ttccgccggt caacgtaata ctcacccgga gcgtccagtt
      421 cttctaaaac attcaggaaa tagaacctct gtccaacccg gtagctgtag tttccgtgcg
      481 gcgggtgtgt ggtgacggtt ctggtatgcg ggtcgatgtg gcgcacacgc tcgtagctgt
      541 ttgcccaatc gtacgaccag tagccgtgga cccagatgtc gtcatccggt tcccacgcca
      601 acggacgatc tccttggtac gtgaacccac cggtcaggtc cccagttgtg gttccccatt
      661 catctgtcag cggtttgtcg aaccccgcga ttttcaggaa ttcccccgat tggggccatt
      721 gagcgagcgt catgggctcg ccttcgatga ataactcggc gtgggcaggc aacacgggcc
      781 gggcaaatcc acgcgaagtc agcgtgccac agtcggtaac accgtattcg ctcagatccg
      841 ccgacagaac gcgatctcgc acggacggtt gcagacgttt gcgcacggat gcatcatcca
      901 cacgcgaaaa gtggggaatc atcaccccgc caatgatgcg ggcactctct ccgggccagg
      961 cgcggtaggc gatcggggat gattctttcc ccgagtcttc ttctgtgagt tcgaaagcct
     1021 tctgccgctg atacacgccg ccgcgaatcc acaccgttac accgccttcc ggcaatcccg
     1081 cggatgcggc caaagccctt atctggtccc gggcgcgctc cagtgtggca aagggtgcgc
     1141 aagccgatcc ctcattggcg tcgttgccgt gcggggcgac gaaaaactcg gacggtgtac
     1201 ctgacatctg agttcctttg gtatcagttt gcctgtttcg cgtgtctgtg tgcgaggcac
     1261 gcaaggatat cttggtcgct ggattgcggc tcggcaactc ctgcgccgca gaaacaggac
     1321 gatgctgaca agaggaagtg tgccctgaaa cgggtctctg cgatcttcga actgctatag
     1381 ttgcgcccat gcgcgcgccc gccaaaccga cggccgccac tctcttcccc ccgtttctcc
     1441 gactttcccc tgtctcttca ggcggtgggg gcagctactc ccgcgcacgt tgtgcggcat
     1501 cgttccatac gtattcgccg gtagatttca cgaaaacgct cagccgcaac tcggaaaacc
     1561 atttccgaaa cagacgttcg tcgttcagaa cggcctttcg ggcggctttc acggcaaact
     1621 ccaccaggaa acgccaaaga aacaccgcgc aaagagcaca gagggccgcg agaatgtaca
     1681 gggaattcca agccgccaga ctggctcccg cgacggcgac aagaatcgac acaaccagca
     1741 tccaacccgt cgcgcgcgcg gcgaaccgac attctgcacc cgaaagaagg acccagaaca
     1801 aaacaaacct ccggtccagc ccgattacga tctcatggtt gcggaaagcc cttcggaggt
     1861 tgtcgaaatc acgccagtgg cgcttgagta tctcactgcc cgccatgtgc tccgtcgccc
     1921 ccggccggaa acccatcttt caggaaatgg accactttgg aaacggtgtc gggagggatc
     1981 ttcgagcgcg cattgcggag ctcggctagt acataccgcg tggttttcgg gatgtccatc
     2041 ggaggcgaga tttcttcaat cagcttggcg atctggcgca cgtcgcgagt tgggcagttc
     2101 aggtatcgcg caacttcccc gtgtgacgct ttcatctggt ccgcgccgtt cggagcgccc
     2161 cgggccacca gacgcggggc gcgcgaaact cgagggcgcg cggagacaac ggcgggggag
     2221 tcgtccttct cttcttcgtc ctcaccatct tgcggttcgt cggaacgttc cagccgcacc
     2281 tcaggctcat cttcatcttc aacacgagcg cgcagctcgc gcacaggcaa cgcgcgacgt
     2341 ggagtctcgg tctgacttgt tttctcgcga accggcggcc gcgcaggaga aaccatttct
     2401 ggcgctactg aagccgcatc ctgcagaacg ggtgtgcttt tctcagggct attcttcggc
     2461 tcatcgtgcg tgattctcgc ttgttcggtc accggcgtgt cgatcgtccc acgctggagt
     2521 gcggtgatcg cacaggatgc acacatgttc gccgagcggc cgtccaacgt aagttgtgca
     2581 atattgggaa aaaagttgcc gcaccgatcg cacggcacaa attcccgaag cagggcgtca
     2641 agttgctggt gcagccgctt tgcttcctga aaagtcgcgg cggaaatggg gcgtttcccc
     2701 agatttgcca gttcggcggc tatagcggtg atgcgatcgc tgaaaatggg cgcgggaggg
     2761 gacattcagc tctccggaaa ccgttacgag catgggcacg atgggtgtgt acgtcggaag
     2821 atagcagggg agccgaaccg cggcaagccg ggactcactc cgtactgccg gttgcagcgg
     2881 caaatggtac agtccttcgg gccacatgct tcagcgagtg ccacgggcga acagaactcg
     2941 tgaatcccac agccggggat cctgaacaac tggatacaca cccacgaaaa tgcccgatta
     3001 ccattgtcct cgaacgccgg gtccatgtcc tgaagcggtt cagctttgag acgaacctcg
     3061 ttcgctgaac gtaacgcagt aggtctgcag ggaacagcgc gagcagttgc tgatgtccat
     3121 cttcaacggg caatcgccaa gaatcccgag gcgactcaga gcgtaatcat accggatcgg
     3181 gtccgccggg tcgaagcgcc gaagctttcg cgtaacctcc aaagccatct tccagtcgat
     3241 cgtggatcgg cgggtgaggc ctatctgcct gcagatccgg gcaacgtgtg tgtcgagggg
     3301 caacacgagc aacgacggta aaacggttct ccagagaccc aggtcgggag atgcgcgccg
     3361 gactacccac ctcaaaaaga ggttcatgcg tttgcacgcg gatccgtccg cgggtgacgg
     3421 cagcagatgc ctgaagaagc gtcgttgctc gatccttcgc cctccctgtt cccgcagaaa
     3481 cgacacaaac gcgtgcagcg caacaccgac atccggtgcg tctttcggta atccgcggca
     3541 gaacgcgcgt tccagcgagc catgtttcct caggacttgc gcgaggatcg aggacattat
     3601 ggccacgtcc tctcctgtgt ggagtcggtg tcgccatccg gtaagacatc tcgcgtcgtc
     3661 cgagtggtgt gaggcggtaa cgaacgcata gggtccgccg ggcattcggc ggaaaagatc
     3721 acgcagattc cgcacgatga tcctggcgtt gccgtatgcg aacatcgcgg agaagaacgc
     3781 tgcaatctcc tgatcccgcg ggtcggagaa ttcgcggact acgacgagcg ggtccggatc
     3841 cagtgccgat tcgtcgtaag tgttgcacag gttctccaag gcggtaccga ggtccgatcg
     3901 cactgaatcc ggatttgcgg tcgtgcggtc acgtgccgtg cggttggtct tggcgaccgt
     3961 cgttgtcctc acggcgtgtg ccgcggcggc aggccggtgt acatcgttgc gacggcgcaa
     4021 ggggcgttct cacctgagag ggacttcggc acccatggaa cacgcgagga gcttcagggg
     4081 gtgcaacgtc gttttcctcg ttccgaactc catctggagt ttgcatgtgc tgcagtctgt
     4141 cactccgtag ttgatcgagg gatcagccag atggtcaagc agcctcttgc cgattgccat
     4201 tgaggtttcg aaatgcgcgc gtttcatgcc gtatgttccg gccataccgc aacagccttc
     4261 atcgagaatc tgtacccgga gcgacgggat gcgcctgagg atgtctactg ccggcagccc
     4321 aacccgcatc gccttcaggt gacagggtgc atggtatgcg acactgaaat ccagtgcccg
     4381 tagaggaagg tcagtgccag tacgcagtct ttgttcgatg aattgagagg cgtcctgcat
     4441 acagccggcg accaactcgg cagcgctgga gccgagaatg tcacggtact cgtccttgag
     4501 aaagagcgtg gccgtaggtt ccgtggcaat gatgcgcagg cccgcctcgg catacgggcg
     4561 caatgcgtca acattgtgcg acgcgagtcg gcgtaccctc gtgagcccgc cgttggcggc
     4621 ggcagcaatt ccgcacggga gagtctccgg cacgatcata cggattccga gcctgttcaa
     4681 caccgcgtgc aacgcttttc ctacatcggg gtcatggtat tgcgcccact ggtccgggaa
     4741 gtacacaacg gcctcttccc ccgcagattg ctcattcttt ccgtgtgtgt ggtgccacga
     4801 cgagaaggtc tttccagcga accggggcag tctccgccgg tggtcaatcc ctgagaacag
     4861 gtccagtgcg gctccgaaca acggttgtcg cgcaaccacg ttggaaaggg gtgagaaaaa
     4921 cctgcctgcg ctactgagaa agtcggatgc tcccattgcg atttcagcga tggaacggcc
     4981 tttgtgttcg cagtactggc cctttgcaat caacatgatg cgcccgatgt ctacgccgga
     5041 agggcattcg aacgagcaca tgcggcagcc gatacacgtg tccagtatcc gtttccagtc
     5101 tttggtcttg cgaaaggatt ccggcaactc gccacttgca aaaagacgga gcaggttcgc
     5161 tttcgcgcgc ggagtggcgg cttcgtctcc gagtgcctta tacaccggac acatggtgac
     5221 aggcggttcg ggtatgcggc acgttccgca tccgtggcag cgcgatatta tgtccgtgat
     5281 ctcatcaggt gctaccggga gaggcgtgcc cagaggttga agagtgtgcc cgctgccgag
     5341 ccgcaggtcg ctgtccaacg cgtggggcgc acgggtcact tttacgtccg ggttcaggat
     5401 gttcgacggg tcgaacaact ctttgacgtc cacaaacacg tcgctgagtt cgccgtacat
     5461 ttggcgaaga aaaggagccc gcgctctccc gtcgccgtgt tccccgctca tggagccgcc
     5521 aaaccgacac accgccccga atacggcact cgcaagcggt tccaatttgg cgatatcacg
     5581 cgcatcggtg aggtcgagca gcgggcggac atgcagattg ccctgacctg catgtccata
     5641 tatcgccgct tcgacatcga agccctggaa aatggctttc aaatcgccga tgaatgttcc
     5701 cagtgactcc ggcggcacaa ttacatcctc tacgaatgcc acgggccgct tttttccggg
     5761 caaggtgtaa aggataggca gggttgcttt ccgcacgttc atgagtgcac tgtgagtcgc
     5821 tgcgtcgaac gcgaacacat gtcccgtggc gagatcgtga gaccgttcca cagccattct
     5881 gcacgcggat tccgtggtct ccgaattgtc gccgtcgaac tccacgaaca actgtgcaac
     5941 tgcatcagtc ggcagaaacg ctgtaagttc gggacgatgg gcgcgtatca tgtccagcgc
     6001 ggtctggtcc atgatttcca ccgcactggg gctcagcgga aggatcgtat gtacagcctc
     6061 cccggcagcc tccacgctgg cgaaattgac cagcaccatt gaagatgcaa gcggcttagg
     6121 cacgatctcg agttcggcct ccgtgacgat gccgagtgtg ccctcagaac caactatgag
     6181 gctcgcgagg tctacgccgt caggtctcag tgctttggcc aactggtacc cgctggagtt
     6241 cttcgcgcac ttcggaaaca tctcacggat gtgggaatcg tgttgccgga ggatcgccgg
     6301 aatctcgtca accaaacggg tcagcagaga tgtctgcggc gatatcggca cggcttctga
     6361 accgcggaac tccacaactg acccgtcggc gagaacacaa cgcaccgcgg cgacgtaatc
     6421 gatcgtggac ccatacttga cggaacgcgc gccgctcgaa ttgcatgcaa tcatgccccc
     6481 aatcgttgcg tagtccccgc ttgaggggtc tggtgggaac atcaatccgc gagccgcgag
     6541 atgcctgttc agcactccaa gtgtgatacc gggctggacc ctcacgcggc gccggttctc
     6601 gtccagttcc agaaccgcgc gcatgaagcg actgaaatcc agaatgaccc ctcgaccaag
     6661 cgcctgtccg acaagtgaag acccgccacc gcgggcggtg acagggatgc ctgtctcggc
     6721 tgcgcgacgc acgacggcca ccacttccgc ttcggattcc gggaacacga cggtggacgg
     6781 ggtgatctgg tacatacagg cagcggtgct gtactgggcc cgtgtcagcg ggtccgaagc
     6841 cacgtggcgg gcacaccctg agaaatcgcg ggttttctgt tcaacaatag ccatcgggtg
     6901 ctttcagaaa gaggtttctc gcggtttttg tgccgaggga tccgggagga gcccttttca
     6961 aaggtacgga gcgcgggcca cctggtaaat cgccggatgt gcgggcaatt ccgtcccgta
     7021 cgtttgccgc cgacgacagc gaggggtatt ttctggtggg gcgcacggcg gccgaacatt
     7081 cgtcaacagg cagtttgagg gggatccgaa tgaacagcgg cattctggca gtaatcctcg
     7141 gaggcggaca gggcgctcgt ctttaccctc tcacgaaagt gcgttcgaaa cctgccgttc
     7201 ctatcggcgg caaataccga ttgatcgata tacccatctc caattgcctg cacagtggga
     7261 ttgacacggt ctacgtcctc acgcaattca acagcgtttc cctgcacaga cacatccacc
     7321 gcacctaccg gttcgatgta ttcggaaggg ggttcgtgga aatcctcgcg gctgagcaga
     7381 cgatggagaa cacgggctgg ttccagggta ccgcagatgc ggtgcgacga agccttcgct
     7441 acatcgattt gcccgactac aaacacatcc tcattctttc cggcgaccat ttgtatcgca
     7501 tggattaccg ggagtttgtt gccacgcacc tccggagcaa caacgacgtg acggtttccg
     7561 tcaaacctgt cggtctcgag gagacgtccg gtttcggaat cgttcggatg gacgaaagcc
     7621 gggaaatcat cgagtttctg gagaagccga cggaaccggc agatcttgaa cgattgcgcc
     7681 ttcctgatgc acctcccggg aggaatttcc tggcgtcgat gggaatctac gtgttccgtc
     7741 gggaggttct ggtggatctc ctgaaccgcg gaactgagga ggatttcggc cgacaggtga
     7801 tccctcgcgc aatcgccgag acgcgtgttg gcgcgcacct tttcgacggg tactggcagg
     7861 atatcggcac gatcgggtcg tttcatcgag ccaacattga ccttgcggcg gagcttccac
     7921 ccttcaattt ctacgagccg ggggcgccca tttacactca tccgcgattc cttcccagct
     7981 ccaagatcaa caatgcgcac gtccaacaga gcatgttgag cgaaggttcg atagtgatgg
     8041 aagcggacat tcagcagagc attatcggta ttcggagtat catccagagg cacgcggtca
     8101 tacggaaatc agtcatcatg ggggccgatt tctacgaaac tgacagggac cgcgcccaca
     8161 atcggtctat cgggcgtccg gacgttggcg ttggcgagaa atgtcttatc gaaaacgcca
     8221 ttgtggacaa gaatgcacgc atcggggacg gagtgcagat catcaatgcg gcgaagatca
     8281 acaacgcgcg ccacgccgat tacgttattg ccgacggtat cgtcgtgatt cctaaggacg
     8341 cggtgattcc accgggaacc gtgatttgaa gccaccgatt gacagagcgt ggtgtgggat
     8401 gactggcctg cctctcgtct tggaaaacgt atcgaagtct tttggaccaa cccgcgccgt
     8461 tgacaatctt tcgcttgctc ttcgggctgg atctgtgtgc ggattacttg gtccgaacgg
     8521 gtcagggaaa accaccacca ttcggatgat catgggtatc ctgttgcccg attcagggga
     8581 tttgcgactc tggggagctc ccgccgaggg cgcggttcgg aaacacgtgg gatacctgcc
     8641 ggaggagcgc ggcctctacg caaccatgac tgttttcgac catctgattt tctttgccga
     8701 attgcacgac ctatcgcgcc gagacgcaaa agacgctgtc gcagaatggt ccgaaaggtt
     8761 tgcgacgaat ggtgagtcgt cgccgaagtc gtggtggggg cgccgcgtac aggagctatc
     8821 caaagggcag cagcagactg tccaactcat cggaacgctt ctccatcgcc ccgggcttgt
     8881 gatccttgat gagcccttta gtgggctcga ccccgtgcac acgggccatc tgcggacgat
     8941 catacgctac ctcaaggagt ctggcgtcgc ggttctcctt tccactcacc gaatggaaca
     9001 agcagagcgg ctttgcgacg acatatgcct catccaccat gggaaggcgg tgctgtcggg
     9061 cgcactgcgt gaggtaaaat ccgcctccac gagccggttg gtggaactgc gtatggaccg
     9121 tgttcccgac tttctggaac ccgatgatct gatcgctgcc gtcgaaatag tctccgatgg
     9181 cgcgcggatc cgtcttcggc agggtgcgga cgcgcagatg gtgcttgacc gcgcgattcg
     9241 gcgagggcgc gtgctg
//
LOCUS       DAOSOO010000121         9165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7516_length_9165_cov_5.929308, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000121 DAOSOO010000000
VERSION     DAOSOO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7516_length_9165_cov_5.929308"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLT86_09305"
     CDS             <1..894
                     /locus_tag="PLT86_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOT36849.1"
                     /translation="VFNRTVSANLGMSYSISNVLAEAGLENIARWIPFDIDEGDLRNR
                     IKNKMIRPTTIPQLLDELKLEQAIAREALRLAFIQHKQLAVGLKGIAKERTLSDVFSQ
                     ESDGSLINMMELDLLVGSGGVLSHAPRRQQSMQMMIDAFQPEGVTRLAVDSIFMMPHL
                     GVLSTVNETAATEVFEKDCMIYLGTCVAPRGKGKKGNQVLSFEMEFQDGRRESGTMNV
                     GDLRHFPLGLNETAKLRATPAKGWDLGHGSGKPVEAVIHGGVVGIVLDTRGRPIQFAA
                     DRPAQIADTVRWNKATDQFPV"
     gene            912..2033
                     /locus_tag="PLT86_09310"
     CDS             912..2033
                     /locus_tag="PLT86_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36850.1"
                     /translation="MAHSYTPGLRVTPFATLQRRRILPLKGEVTVRVGDLVSRDTVVA
                     RTELPGDVATVNVVNRLGIEAHQIEQYMVKKVGDSVATDELIAETRPFFKFLKSTVNS
                     PIDGTVESVSTVTGQVILRRPPKPVAVTAYVDGRVIEEIPNEGVVVETQGAFIQGIFG
                     IGGEAWGKIRRVVESHDSVATPSSISADMEGCVLICGSIVSADTIKRAIAVKAAGVIA
                     GGIHAGDLKDLLGYDLGVAITGTERIGTTIIVTEGFGAIAMARRTFDILSSCEGMVAS
                     INGTTQIRAGVQRPEIIVSRPPSPSDTEVAASSAGLQIGDPVRVIREPYFGRLGSVSA
                     LPSELQTVESETRVRVLEVALEDGEKVIVPRANVESIAL"
     gene            2109..3407
                     /locus_tag="PLT86_09315"
     CDS             2109..3407
                     /locus_tag="PLT86_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36851.1"
                     /translation="MQRLTQFACIAALFWTGTALASDAVETATGSKTPLPDSVRALPP
                     PLPTQIRAADVPSDNGKAVVVTWLPDASTPSLVRYQVGVATNPSGPYYFGGEAPAAGP
                     IVKDDPKIFGWSPANPDHQFLVVSSYMVPPATPDGNPERVELRPDQTYYLRFAVVGDS
                     ASAELAAPMSVTPRQNWIKLHKLNNLIFGLFFGVVILSFIEFARRNPNMFIRKIAGLD
                     AVEEALGRATEMGKPVYFVHGLNSMGEVSAIASVNILGRVARRVAQFDTTLKVMNNDP
                     IVLAVSQEVVREACIDVGRPDAYVPDNVMFVADRQFSYAAAVDGMMMRERPATVMLMG
                     YFYAEALLFAETGNVVGAIQIAATDSYTQIPFFITTCDYTLIGEELYAASAYLAREPK
                     LLGSLRGQDVGKAVLIAILTIGTALELAGIHWLTHLVAPL"
     gene            3441..4094
                     /locus_tag="PLT86_09320"
     CDS             3441..4094
                     /locus_tag="PLT86_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36852.1"
                     /translation="MIFFKRTLPLVIAFVMGIVAISIYYIPHQAAVDLNDLLSKWDRI
                     IAAFAAFLGVYSLLYLHAGRLKRRQAGWGYSVFVYLGFVVMLTAAIYNNGAGPLVALR
                     KGGAYDWLYTNVFVAADASMFAILSFFIASAAYRTFRARTTEATILLVAAVIVMLGRV
                     PIGALLWESMPELQQWLMNVPNMAAKRGILLGVYLGAVATALRIIFGIERSYLGGDE"
     gene            4091..4951
                     /locus_tag="PLT86_09325"
     CDS             4091..4951
                     /locus_tag="PLT86_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36853.1"
                     /translation="MIQKLLNIDRRWIFLFIFVGVAAAMLFPLPLPIRPSSDVKNVYN
                     TIESIKGKPNATVLLSFDYGPGSEPELQPTAISIIRQCFRNNIKVVGMALWPDAVGLA
                     QAAFDSVSRAEGKEYGKDWTFLGYKTGSVTLILNMGEDFHGAFNVDYRGNKTSEMPVT
                     QHIAKLGSFDYVVTLAAGKTIDGVWVTYAVDRYRIKLGGATTAVTSPDMFPYLQSGQL
                     NGLVSGLAGAAEYETLVRTQGSATRGMQPQSAAHLIIIISIIIGNTMYFVQRRRDRRQ
                     SVSGNEGTRS"
     gene            4948..5826
                     /locus_tag="PLT86_09330"
     CDS             4948..5826
                     /locus_tag="PLT86_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36854.1"
                     /translation="MMHNPGSRTATFVFAGIFLALSIWQLAAGHIALTVDGLGVLTGA
                     GLTLALFSFLYDDNPLFKIAEHLYVGVAAAYGLALVWFQVILADLVNPIFFSTATIRQ
                     LWVLIVPSVLGILLIMRIVPKFSWLSRISFSYVVGFGAGISIPAYIASQLLEQVHPTL
                     APALGWDGGDKFILIAAIVLIAVAYLVSRRGGGDLGMNSWFGPTLLSVVFLICYFQGV
                     GAASLVLVGVAAVLFYFFFSVEHTGAVGAISRVGIWFLMIAFGASFGFTVMARISLLI
                     GRMQFLLGDWLHLLKI"
     gene            5853..6416
                     /locus_tag="PLT86_09335"
     CDS             5853..6416
                     /locus_tag="PLT86_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT36855.1"
                     /translation="MECLRIGTRVCECLDGDITEQDTDAIVNAANNHLWMGAGVAGAI
                     KRKGGQGVEEEAVRLGPVAVGDAVVTSAGLLRAGHVIHAACMGQDLRTDREAIRRATL
                     QSLRRAEELKLRSISFPALGTGVGGFPTAECARIMLTEAAEFLKTAATMELIRFVLFG
                     KESAAAFDTELRNIRERLASSGSPPSC"
     gene            6599..9001
                     /locus_tag="PLT86_09340"
     CDS             6599..9001
                     /locus_tag="PLT86_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT36856.1"
                     /translation="MAWIFRCSEPVADLVRLSRSIELPVDQHQSLVSKGDLVFLWEGG
                     TNGTLTAVGILQSAPYRFDRNSRRQRARVGVSIVLAQPLTAEECRQDQILNALAVLER
                     SDQTTLGLNRVTKDRLLSVLAAREPALAEKLNLPPNAIRPAHEPIPGPSNRDTGAENE
                     KDGTSPVREEFDLEAVHNQVREWLSDPLVPVLLRLVRSNRERLRTLLRPEVLADIEYE
                     AFIAEFGSPGRIQWAGEDVTFMEISERTGTANTEYLDGLLADGTVSTRGNLVWTQFDR
                     DSAKRAEQLDPNSRSLRLRAALAHLLAEGRSLGVRFDRMRRSFDELWPEMATGILSVI
                     EPDEYVPFDRPALAGLQVLGISRRWRNTTDDFTVYCDLVKRVCIAGNLPDLASTHVFL
                     TYLGNRSSGADSAEPADAVRQTHSEDSAARADRRSQAPAPEDAPAHLPTEGQAVFSLY
                     QSVRRRGVVTTLAQVISLYLSVKTVPFVCFEGVPGSGKSHLIRSFAREIGGRLYSCPL
                     TNESVGGSSDAPIRFRDLLGIVDILGRRFYPDVMYRALSHAHQRSSDLVFVFIDTPEG
                     WQEDGWLAEYTRLLDLRAEHDIEGRAVSVLPSGGLLEDSDYPAEFPFPGNVVLVLSVA
                     GHGACGEIASERANVFTLRAPDLSLEALVAGPVPEGASPTTLAAFLADHHRIQPAATP
                     DYAALEVWNREIIHMNSFLEPLGIPVGYRLRHEILRFLAYSEELSAQLPYGVKYPLET
                     AFDHQICQRILPRVLTLLEKDEQVGDVVGELLSYAQGSRGPTPRFPHAAARLQAVQRR
                     ME"
     gene            9010..>9165
                     /locus_tag="PLT86_09345"
     CDS             9010..>9165
                     /locus_tag="PLT86_09345"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT36857.1"
                     /translation="MSDSATIATEKHSSASLKDLKAHGTKITMLTAYDFPTAQFVEAA
                     GVDVALVG"
BASE COUNT         1867 a         2604 c         2721 g         1973 t
ORIGIN      
        1 gtgttcaacc gcacggtgag cgcgaatctc ggtatgtcgt acagcatttc caatgttctg
       61 gccgaggccg gactcgagaa catcgcgcgt tggataccgt ttgatataga tgaaggtgac
      121 ttgcgtaaca gaatcaaaaa caagatgata cgtccaacga ccatccctca gctccttgat
      181 gaactgaagc tggaacaggc aattgcgcgc gaagccctgc gactggcatt catacagcac
      241 aagcagcttg ccgtgggttt gaaaggcatc gccaaagaac ggactctttc tgacgtgttc
      301 tcgcaggaga gtgacggcag cctgatcaac atgatggagc tggacctgct ggttggctcc
      361 ggtggcgtcc tcagccatgc cccgaggcgg cagcaatcca tgcagatgat gattgacgcg
      421 ttccagccgg aaggtgtcac acgccttgcg gtggacagca tcttcatgat gccccatctg
      481 ggcgtgctgt cgaccgtcaa cgaaactgcc gccactgagg tgtttgaaaa ggattgcatg
      541 atatacctcg gcacctgtgt ggcacctcga ggaaagggga aaaagggtaa ccaggttctt
      601 tcattcgaaa tggagttcca ggacggacgc cgggaaagcg gcacaatgaa tgtcggcgac
      661 ttaagacatt tcccactcgg tttgaacgaa acagcgaagc tccgggcaac acccgccaaa
      721 ggctgggacc tcggacacgg atcaggcaag ccagtggaag ccgtaatcca cgggggagtc
      781 gtcggaatcg tgctcgacac gcgcggcaga cccatccagt tcgccgctga tcgcccggcg
      841 cagatcgctg acaccgtccg ttggaacaaa gcaaccgatc agtttcccgt ctaacccctg
      901 taagggtatc tatggcacac tcctacactc ctggcttacg cgtaacaccg ttcgcgacgc
      961 ttcaacgtcg acgtatactc cccctgaagg gggaagtcac ggttcgagtt ggcgacctcg
     1021 tttccaggga taccgtggtc gcgagaactg agcttccggg cgatgtggct acggtgaacg
     1081 tggtgaaccg cctcgggata gaagcccacc agattgagca atacatggtg aagaaggtgg
     1141 gcgattcagt cgcgacggac gaacttatcg cggagacgcg cccgtttttc aagtttctga
     1201 agtccacggt gaactcgccc atagacggca cagttgagag tgtttccacg gtcaccggcc
     1261 aggttattct gcgccggcca ccaaaaccgg tcgccgtaac tgcgtacgtg gatggcaggg
     1321 taattgagga aatcccgaat gaaggcgtag tggtcgaaac gcagggcgcc ttcatccagg
     1381 ggattttcgg gatcggcggc gaagcctggg gcaagattcg gcgtgtggtg gaatcccacg
     1441 attctgttgc gacaccttcc tcaatttccg ccgatatgga aggctgcgtg cttatctgtg
     1501 gatcaatcgt ctccgcagac acgatcaagc gggcaatcgc ggtgaaggcc gccggagtga
     1561 tcgcgggcgg gatccacgca ggcgacctga aggatctgct tggatatgat ctgggggtgg
     1621 caatcacagg aacggaacgg attggaacca cgatcatagt caccgaaggc ttcggggcga
     1681 ttgcaatggc gcggcgaact ttcgatatct tgagttcatg tgaaggcatg gtcgcctcca
     1741 tcaacggcac cacacagatc cgtgccggcg ttcagcggcc tgagattatc gtttcgcgtc
     1801 cgccgtcacc atctgacacg gaggttgcag caagcagcgc gggtctccaa atcggcgacc
     1861 cggtgcgcgt gattcgcgaa ccgtatttcg ggcggctggg ttcggtaagc gctctcccct
     1921 ccgagctcca gacggtggaa tccgaaaccc gtgttcgcgt gcttgaggtg gccctcgagg
     1981 acggagagaa agtcatcgtg ccgagagcca acgtggaatc gatcgcactc tagtgcattg
     2041 ttctggggtg agtccagtga taacacccgc gccgtccgcg tcgtccggag gtgccaggag
     2101 gacaaccagt gcagagattg acgcagttcg cgtgtattgc agcgcttttc tggaccggga
     2161 cagcgctggc gtctgacgcg gtggagaccg cgaccggcag caaaacgccg cttcctgatt
     2221 cagtccgtgc actacccccg ccccttccta cccagatccg agccgcagac gtacccagcg
     2281 acaacgggaa ggcggtggtg gtaacctggt tgccggacgc ttcgactcca agcctggttc
     2341 ggtaccaggt tggcgtggct acgaacccgt caggaccgta ctacttcggc ggcgaagcac
     2401 ctgccgcggg gcctattgtg aaggatgacc caaaaatctt cggttggtct ccggcgaatc
     2461 ctgaccacca gtttctcgtc gtttcatcct acatggtgcc gccggcaacg ccagatggca
     2521 acccggagcg cgtggaacta cggccggatc agacgtacta cctgcgcttc gcggtggtcg
     2581 gcgattccgc gagcgccgag ttggccgctc cgatgtccgt gacaccgagg cagaactgga
     2641 tcaagctcca caaattgaac aacctgatat tcggcctctt tttcggcgtg gtcatcctct
     2701 ctttcatcga attcgcgcgg cgcaatccga acatgtttat acggaaaatc gccgggcttg
     2761 acgcggtgga agaggcgctg ggacgcgcca ccgaaatggg gaaacccgtg tatttcgttc
     2821 acgggctgaa ctccatggga gaagtgtccg caatcgcttc cgtgaatatt ctcggccgcg
     2881 tggcacgaag agtagcgcaa ttcgatacga cactgaaggt tatgaacaac gacccgatcg
     2941 tgctggcggt gagccaggaa gtggtgaggg aagcgtgtat tgacgtaggg cgcccggatg
     3001 cctacgtccc ggacaacgtc atgttcgttg cggatcgcca gttcagctac gcagccgcag
     3061 tggacggaat gatgatgcga gagcgcccgg ccaccgtgat gctgatggga tacttttacg
     3121 cggaggcgct tctcttcgcg gaaacgggga acgtagttgg cgcgatccaa attgccgcca
     3181 ccgattcgta tacgcagatt ccctttttca tcacgacatg cgactatacg ctgatcggtg
     3241 aagagctgta cgccgccagc gcgtatctcg cgcgggaacc caaactcctc ggaagccttc
     3301 gaggacagga tgtcggaaag gctgtcctga tagccattct gacgatcgga accgcactgg
     3361 aactggcggg catccactgg ttgacacatc tggtagctcc tctctgaatt tgcggacgcg
     3421 agtatggagg ctgagaagga atgattttct tcaaacgcac gttgccactt gtcatcgcgt
     3481 tcgtgatggg tatcgtggcc atatcgattt actacatccc ccatcaggcc gcggtagacc
     3541 tgaacgacct tttgtccaag tgggaccgga ttattgcggc ctttgccgca ttccttggcg
     3601 tgtacagcct gttgtacctt cacgcaggac gcttgaaacg caggcaggcc ggctggggat
     3661 acagcgtttt tgtgtacctc ggtttcgtgg tcatgctgac ggccgcgatt tacaacaatg
     3721 gcgcgggccc gctggtcgcg ttgcgcaaag gtggtgcgta cgattggtta tacacgaacg
     3781 ttttcgtggc cgccgatgca tccatgttcg cgattctttc cttcttcatc gcatccgcag
     3841 cctaccgaac cttccgagcc cgaacgactg aggcgacgat cctgcttgtg gcggccgtca
     3901 tcgtcatgct gggacgcgtg ccgataggcg cgcttctgtg ggaatcgatg ccggaactgc
     3961 agcagtggtt gatgaatgtt ccgaatatgg cggcgaagcg cgggatcctg ctgggcgtct
     4021 acctgggtgc agtggcaacc gccctgcgca tcatcttcgg tatcgaacgt tcctatctcg
     4081 gaggtgatga atgatccaga aactcctgaa catcgaccgt cgctggatct tcctctttat
     4141 cttcgtcggg gtagccgcag cgatgctttt tccgttgccg ctgccaatcc ggccttcctc
     4201 cgacgtgaag aacgtgtata acacgatcga gtccatcaaa ggaaaaccga acgcaacggt
     4261 gcttctttca tttgattacg gcccaggaag tgaacccgaa cttcagccca ccgcgataag
     4321 catcatccgc cagtgttttc gaaacaacat caaggtcgtc gggatggctc tctggcccga
     4381 cgcggtggga ttggcccaag cggcgttcga ttccgtatcg cgggcagaag gaaaagaata
     4441 cgggaaggat tggacctttc tcggatacaa gaccggttcc gtgacgctga tcctgaacat
     4501 gggagaagac ttccacgggg ccttcaatgt ggattaccgc ggaaacaaaa cgtcggaaat
     4561 gcctgtcacc cagcacattg ccaagctggg gagctttgat tacgtggtca ctctcgccgc
     4621 gggaaaaacc atagacggcg tctgggtcac ctacgccgtt gaccgctacc gcatcaaact
     4681 cggcggcgcg acaacggcag tcacgtcgcc agacatgttt ccttacctcc agtccggcca
     4741 gttgaacggg ctggtatcag ggttggccgg tgcggcggaa tacgagacgc tggtcaggac
     4801 gcagggatcg gcaacacggg gtatgcaacc tcaatctgcc gcgcatctta tcatcatcat
     4861 ctccatcatt atcggcaaca cgatgtattt cgttcaacgg agacgagacc gcagacagag
     4921 tgtttccggc aacgagggga cacgatcatg atgcacaatc caggaagccg gaccgcaacg
     4981 tttgtgtttg caggcatttt cctcgcactg agcatctggc aacttgccgc gggacatatc
     5041 gcgttgacag tggacgggct gggagttctc actggcgccg gactcactct cgcgctgttc
     5101 agtttccttt acgatgacaa cccgcttttt aagattgccg agcatctgta cgtgggtgtg
     5161 gcggcagcat acggacttgc gctggtgtgg tttcaggtga tcctcgcaga cctcgtgaat
     5221 ccgattttct tctcgacggc cacgattcgc cagttgtggg ttctcatcgt tccatcggtt
     5281 ctcggcattc ttctgatcat gcgaatcgta cccaaattca gctggttgag caggatatcg
     5341 ttttcctacg tggttggatt cggggccggt ataagtattc ccgcgtatat cgcctcccag
     5401 cttctggaac aggtacatcc aacgttggcg cctgcgctcg ggtgggacgg aggagacaag
     5461 ttcatcctta tcgcggcgat agttcttatc gccgtggcat atctggtgtc gcggcgtgga
     5521 gggggcgatc tcggaatgaa cagctggttc ggtccgacgc tcctttcggt cgttttcctc
     5581 atctgttact tccaaggcgt cggggcggcc tcacttgttc tggtcggtgt agccgcggtc
     5641 ctgttctatt tcttcttttc cgtggagcac accggtgcgg tcggagccat ttcgcgtgtc
     5701 ggaatctggt tcctcatgat cgccttcgga gcatcgtttg gtttcaccgt gatggcgaga
     5761 atctctctcc tcatcggcag aatgcagttc cttctgggcg attggctcca ccttctcaaa
     5821 atctgatttc gtcaaaccgg ggatagcggc gcgtggagtg tctgagaatc ggtacccgag
     5881 tatgcgaatg cctggatggc gatatcacag agcaggacac tgacgcgatc gttaatgcgg
     5941 ccaacaacca cctatggatg ggtgcagggg tcgccggcgc aatcaagcgc aagggcgggc
     6001 agggcgttga ggaagaagcc gtccgcctcg ggcccgttgc ggtgggagac gctgtggtca
     6061 cctcagcagg acttctccgc gcagggcacg ttattcacgc tgcctgtatg ggccaggacc
     6121 tgagaaccga ccgggaggca attcgtcgag ccacccttca gagcttgcgc cgggcggagg
     6181 agctgaagct tcgcagcatt tcctttcccg cgctcggaac cggagtcggg ggattcccga
     6241 cggcggaatg cgcgcgaata atgcttacgg aggcagcaga attcctgaaa acggccgcga
     6301 caatggaact catccgattt gtgctgttcg gaaaagaatc agccgcggca tttgacacgg
     6361 aattgcggaa cattcgagaa cggctcgctt cttccggttc tccgccctcc tgctgaccgc
     6421 gggcctgcct gcgacaccgc tgcttcagac tccgcacgcg atcgtatgct tcccttcttc
     6481 ttgcctgtca atccccgggg tggtacgttt atgatgattt ccccgtaatc cccgaaggta
     6541 ccgcggactt cgcgcgttca tgcgaagaac gtgcttctca tcgtcttcaa ggtgtgccat
     6601 ggcatggatt ttccggtgct cagaaccggt tgctgatctc gtccgcttgt ctcgttcgat
     6661 cgaacttccc gtagaccagc accagtctct tgtcagcaaa ggcgatctcg tgttcctgtg
     6721 ggagggtggg accaacggga cactgacagc cgtgggaatc ctccaatctg cgccctaccg
     6781 cttcgacaga aactcccgca ggcagagagc gcgcgtcgga gtctccattg ttctggcgca
     6841 gccgcttacg gcggaggaat gccggcaaga ccagattctg aacgctcttg ccgtattgga
     6901 aaggtccgac cagacgacct tgggtttgaa tcgagttacc aaggaccgtt tgctttccgt
     6961 gctggccgca cgtgagcccg cgcttgcaga aaagctcaat ctcccaccaa atgcgatcag
     7021 gccggcacac gagccaatcc ctgggccaag taatcgggac accggcgcgg aaaacgaaaa
     7081 ggacggcacg tcgcctgtcc gcgaggagtt tgacctggaa gcagttcata atcaggtacg
     7141 agaatggctt tcggatcccc tggtacccgt tctattgcgg ttggttcggt caaacagaga
     7201 gcgactaagg acactgctgc ggccggaagt gctggcggac atcgaatatg aagcgttcat
     7261 cgccgaattt gggtcgccgg gcagaatcca atgggctgga gaagacgtca ccttcatgga
     7321 gatttccgaa aggacgggca ccgcgaacac ggaatacctg gacggcctgc tcgccgacgg
     7381 aacggtctcg acgaggggta acctcgtctg gacgcaattc gatcgcgatt ccgccaagag
     7441 agcggaacaa cttgacccaa actcgcgctc attgcggctg cgtgctgcac tggcgcacct
     7501 gctggcagaa gggcggtcac tgggcgtcag gttcgatcga atgcgccgat ccttcgacga
     7561 gttgtggccg gaaatggcca ccggcattct ttccgttatt gaaccggacg agtatgtccc
     7621 gtttgaccga cctgcgctcg cggggcttca ggtgctgggc atttcccgtc gatggaggaa
     7681 taccaccgac gacttcaccg tgtactgcga tcttgtcaaa cgggtttgca ttgcggggaa
     7741 tttgcccgat ctcgcatcca ctcacgtgtt tctgacctat cttggaaatc gcagcagcgg
     7801 cgctgattcg gctgagcctg ccgatgcggt gcgccagacg cattccgaag acagcgcggc
     7861 gcgcgcagac cgccggtcgc aggctcctgc tccggaggac gcgccagcac atctgccgac
     7921 tgaaggacag gcggttttct ccctgtacca gagcgtccgc cgtcgcggag tagtcacgac
     7981 tctggcacag gtgatttccc tgtatctttc ggtaaaaaca gtccctttcg tgtgttttga
     8041 aggagttccc gggagcggga agagtcacct gattcgctcc ttcgcgcgcg aaatcggcgg
     8101 acggctttac tcctgcccgt tgacaaacga gagcgtcggc ggatcctcgg atgcgcccat
     8161 ccgcttccgc gaccttctcg gaattgtcga cattctcggc cgccgctttt atcccgatgt
     8221 catgtaccgc gcgctgtcgc acgcccacca acgctcctcg gacctcgttt tcgtgtttat
     8281 cgacaccccc gaagggtggc aggaagacgg gtggttggct gaatacacaa gacttctgga
     8341 cctcagggca gaacacgata tcgaggggcg tgctgtttcg gttctccctt ctggaggcct
     8401 tctggaagat agtgattacc ctgcggagtt tcccttcccg gggaacgtgg ttctggtttt
     8461 gtcggtcgca gggcacgggg cgtgcggaga aatcgcttct gaacgcgcga atgttttcac
     8521 gctgcgcgcg ccggaccttt cgcttgaggc tctcgttgcc gggcccgtgc ctgaaggcgc
     8581 ctcaccgaca acgctcgcag cctttctcgc cgaccaccat cgaatccagc cagctgcgac
     8641 tcctgactac gccgcgctgg aagtatggaa ccgggaaatc atccacatga actcatttct
     8701 ggagccgctg gggataccgg tgggataccg gttgcgtcac gaaatactgc gattccttgc
     8761 ctactccgaa gagctgagcg cccaattgcc ctacggcgtc aaatacccgc tcgagacagc
     8821 gttcgatcac cagatatgcc agcgaattct tccgcgcgtt ctcacactcc tcgaaaagga
     8881 cgagcaggtc ggggatgttg tcggcgaact gctttcgtac gcgcagggat cacgagggcc
     8941 taccccccgg ttccctcacg ccgctgcgcg cttgcaggcg gtgcaacgca ggatggagtg
     9001 aatgtcgcca tgtcagacag cgccacaatt gccacagaga agcactcaag cgcttctctg
     9061 aaggatttga aagcgcacgg aacgaagatc accatgctga ccgcgtacga ttttcccact
     9121 gcacagttcg tcgaggctgc aggcgtggat gttgcgctgg tgggg
//
LOCUS       DAOSOO010000122         9148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7535_length_9148_cov_5.797976, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000122 DAOSOO010000000
VERSION     DAOSOO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7535_length_9148_cov_5.797976"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1426
                     /locus_tag="PLT86_09350"
     CDS             278..1426
                     /locus_tag="PLT86_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT36858.1"
                     /translation="MAVVDIRAQAKRRTPEELIRHLQTFSLELNFAAGVWFFAPGGGR
                     FHDRYTPNLSIPERLKLAGELAPYGLKGVEAHYPTEVNEGNWHHYQQLEKDTGIKLVG
                     FGPALFYDKDFEWGALSSPYPEVQEKAIDIFARALKFAKDTGTPHVIHWPGIDGWENN
                     FGIDFIAMRDRFARNLAKAMDRVPGVRVCMEPKPYEPRGRIIYGSTYEGLLLAQKVES
                     LLEHPENKAILAEGHALVAMNPEVGHMLMGFEDLAYSFSLIIEYGKLAHTHWNSQPLG
                     NYDQDMNVGVLSPEQTEAALYVLKMHGYKGYFSPDLNPERMSPLQALKNCFDALRAAN
                     ERINNLDHEKVIMSTTFPRKNRGWLEAYLIRQRAGDVAGLSEMPEFEK"
     gene            complement(1598..2590)
                     /locus_tag="PLT86_09355"
     CDS             complement(1598..2590)
                     /locus_tag="PLT86_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT36859.1"
                     /translation="MARTFTAVVFPEANRAELREFPLRAPRKGEVLARVVVSGVSIGT
                     EYLVLSGQFPNQKFPCLVGYQSVGVVEEVGEGISHLRPGDRISIGSSEIPEGYHEGAG
                     AAHFSHAVIPAEPGNDRAIIVPSHVSDDAASYFVLVSVAVEGIRLANVQRNDLVAVVG
                     QGVLGQLVAQICRHNGATVFTSDLDPARVGISARYSAHRAFALDVPAFDEELRKVQKK
                     GADVVFETTGNTKVFDQALTLARDYGTFVAQGHYPGNLTFRFVHAHWRHLRMMFPCGK
                     GKTWESVETMARGIVNVDSLITHRVSPQDAPDLYAKIAARSPEILGATIRWAER"
     gene            complement(2604..3620)
                     /locus_tag="PLT86_09360"
     CDS             complement(2604..3620)
                     /locus_tag="PLT86_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT36860.1"
                     /translation="MNAVLIERAHELGFREVPEAPMGDYHARTRLVAGSICNSTDAKL
                     LHGTFPGCTAFPAVLGHEAVGEVTAVGAKVRNYRVGDLVIRPRVCFPPEFNIREYFGS
                     FADFAYVTDQWAMDDANPPTGARAFGHPQQVLPAGTDPMLGVLSITLKETLSWIRRFA
                     VGKGHRVLVFGTGPVGTAFVLFSRMAGAKQVILAGRTESSIRRAAELCHPDTSVNVTD
                     PHFVARVHEITNGEGVDRAIEGVGDTSVIDLAVPCLSPEGQIGLYGVAPSTQAKALHA
                     NHPRVRAISPDESEVHEEFFSLVREGRIDAFAFLSHRLPIRDIGRGFSLLANREAFKV
                     GLDW"
     gene            complement(3750..4256)
                     /locus_tag="PLT86_09365"
     CDS             complement(3750..4256)
                     /locus_tag="PLT86_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOT36861.1"
                     /translation="MQVQKKLFHCGDVLLATMDKEIEEVLAAVHAVEWRDSFEYTHGG
                     TVYRHQAAYNLALAAEFGTMGWDPQPVLRTDPKLIGDFRKGLVFVEVQFGNAAALYRD
                     FYKFRYGLANGLLSLAVLVVPSDPVRFFPTRRGSVRNMADFGLADRCLSVLPVNVPTL
                     LLGLLPAA"
     gene            complement(4286..4465)
                     /locus_tag="PLT86_09370"
     CDS             complement(4286..4465)
                     /locus_tag="PLT86_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36862.1"
                     /translation="MKTKRKRFDCVAMKRRAAERIYRETAGLTREQEIEYWRRSIDVL
                     KTRKVVTPETPRATC"
     gene            complement(4666..4836)
                     /locus_tag="PLT86_09375"
     CDS             complement(4666..4836)
                     /locus_tag="PLT86_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36863.1"
                     /translation="MFFEHMHRRAFVMVVSDVVVRELAGAPPRVRALGEKMLPLAEVV
                     PVTQEALALRDA"
     gene            5245..5577
                     /locus_tag="PLT86_09380"
     CDS             5245..5577
                     /locus_tag="PLT86_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36864.1"
                     /translation="MRRTATRLPWMVVHFAFHEHPAGVVCSYRTRYSVMSLFLLLGVS
                     VVFLAFPLQVAGTSVFGWHLSFAWWEYVVAVLSPLVWLWLVQDGRNDARRVITEFQRR
                     LGENFAAG"
     gene            complement(5628..7070)
                     /locus_tag="PLT86_09385"
     CDS             complement(5628..7070)
                     /locus_tag="PLT86_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36865.1"
                     /translation="MPCGLIRVFALLCMLPAWSAAQVYSFEEDAPPDSSVAPLDSAAV
                     ERTWNAVLSGARQHSPGLVRPYVAERYRNTRLVTSLVALGSVQRFRVLRMDVYPAFAR
                     LNLEVTENFRRSYHSFYFVRESGKVVLTYRWHAYSQHWVERRSPHFTFVYNPYKDSIG
                     RGVAVPTNMAATLLERHRAAMANLLNPASTERIYIYLVNTKAEMMSLFGSTADEAFIE
                     PETGAIVSDFPHGVFQLVTTRLLFQVSGDSLGKLNSTGNDRLCRLFINGVSTFGDGNG
                     GYVRGKSAVFMVRTLISQGRYIPLRDALSSPDNPLVVAEAGAFVRFLVSEYGASKFRS
                     AARSIHRDTDFIPVLERVYGVRFPALERDFRVWVVRQNEEVLEPSELVEFRILTHLWD
                     VQRVGGTRVYVDRGMSFPPISAIGRVMTLFQNRVATQPATIPYIYLAASENQLAEFYI
                     QGSAYVSEHVMIAASTEALERALRSRRERR"
     gene            7266..8303
                     /locus_tag="PLT86_09390"
     CDS             7266..8303
                     /locus_tag="PLT86_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36866.1"
                     /translation="MTPREIVQMSLAFAYPPRIPFGMGSAGFPSDFRGVHHDPAPNRV
                     QQPWTQRDGYWDMIDMWGNTWRRLESITKGEVYKGVIQDSWDLLDTYEFPILDRAELF
                     EKAAAEVKQYHAEGYYVLGGMSWTFDTARYMRRMEVFLRDCAEEPDRVSYLLNRIADV
                     YERVIERYADIGVDGISTGEDWGTQDRLLVSPPMFRRLFKPVFARLCGVAKSRGLSIH
                     FHSCGFVRDVIGDWIEVGIDSFKFDQPELHGIDWLAEHYGGKMHIWSPVDIQTTLQTR
                     DHARIEAAAREYIEKLALPFGGGVIAGYYGSNEAIGLTPEYQETASRAFMKYGDPALW
                     TGRPASRTEKA"
     gene            8309..8758
                     /gene="dtd"
                     /locus_tag="PLT86_09395"
     CDS             8309..8758
                     /gene="dtd"
                     /locus_tag="PLT86_09395"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT36867.1"
                     /translation="MRAVVQRVSSAHVAVDGQTVGAIGPGLVVLLGVAEGDSDADVAY
                     MAEKIANLRIFEDAAGKMNRSLLETGGALLAVSQFTLLGDCRKGRRPSFIEAARPEEG
                     KRLYDAFCDAVRAFGVRVETGRFQAMMQVALVNDGPVTILVDSRKVF"
     gene            complement(8940..>9148)
                     /locus_tag="PLT86_09400"
     CDS             complement(8940..>9148)
                     /locus_tag="PLT86_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36868.1"
                     /translation="EAGHVTLAVYDINGRLVRTLVGQAFLPGHHEVVWDGYDAMGRAV
                     ASGVYVYSLTAAQGVVTRRMVLVR"
BASE COUNT         1863 a         2853 c         2706 g         1726 t
ORIGIN      
        1 acgcagtaag gccatcctcc gtcgtctcga tacagcctcc gtttgcgcgg aggccactcg
       61 acgaccgcac agcacgcgca acctcctgga aacgccgtca ttcccgcaaa gccgggaatc
      121 cagaccgcgc gaagtgcaac cggtcgtcgt gtgagaaaga gacgcgccaa tcgggggatg
      181 accgccttcc ctttccccgc accggctgcg tacggatatt cttacgacgg gatgtgagtc
      241 tgcgttcaca ccaaagtcgt caatgaaagg gattcccatg gctgttgtcg atatccgggc
      301 gcaggcgaag cgccgcacgc cggaggaatt gatccggcac ctgcagacgt tctcgctcga
      361 gctcaacttt gccgccggtg tctggttctt cgcgccgggc ggcggccgat tccacgaccg
      421 gtacacgccg aatctcagca taccggagcg gctcaaactg gcaggagaac tcgccccata
      481 cggcctcaag ggcgtcgagg cgcactaccc gaccgaagtg aacgagggca actggcacca
      541 ctaccagcaa ctggaaaagg ataccggcat caagctcgtc gggttcggcc ccgcactctt
      601 ctacgacaaa gacttcgagt ggggagcgct ttcgtcgccg tatcccgagg tgcaggaaaa
      661 ggcgattgac atcttcgccc gggcgctcaa gttcgcgaaa gacaccggca cgccgcacgt
      721 catccactgg cccggcattg acggttggga gaacaacttc ggcatcgact ttatcgccat
      781 gcgcgaccgg ttcgcccgca acctcgcgaa agcgatggac cgcgtgcccg gtgtgcgcgt
      841 ctgcatggaa cctaagccct atgagccacg cggacggata atctacgggt ccacgtatga
      901 aggccttctg ctcgcacaaa aggtggagtc cctgctcgag catccggaaa acaaggcgat
      961 tctggccgaa gggcacgcac ttgtggcgat gaacccggaa gtagggcaca tgctcatggg
     1021 cttcgaggac ctcgcctaca gcttcagcct gatcatcgaa tatggcaagc tggcgcacac
     1081 gcactggaac agccagccac tgggcaacta cgatcaggac atgaacgtcg gcgtgctttc
     1141 ccccgagcag accgaagcgg cgctgtacgt actcaagatg cacgggtaca agggctactt
     1201 cagcccggac ctcaaccccg agcgcatgtc cccgttgcag gcactgaaaa actgcttcga
     1261 cgccctgcgc gcggcaaacg agcggatcaa caacctcgac cacgagaagg tgatcatgtc
     1321 caccaccttc ccgcgcaaga accgcggatg gctggaagcc tacctcatcc gccagcgcgc
     1381 gggcgatgtg gccggattgt cagagatgcc ggagttcgag aagtagcagt ttgagcggaa
     1441 tgagcacaga aatagcgggc gcggcgccgt cccctttttc catggaaact cggtgcctga
     1501 ccgacgacag gatggaggga cacgctccgt cgtgtccctt tgttttcccc cgcccgggac
     1561 gatgccccgg gctgtgcaaa tggcggccct tgaagcctta cctctccgcc cagcggattg
     1621 tcgcgccgag aatctccggc gaccgcgcgg cgattttcgc gtacaggtcc ggcgcatcct
     1681 gagggctgac gcggtgcgtg atgagcgaat ccacattgac gatgcctcgc gccatggttt
     1741 ccacgctctc ccacgtcttg cctttcccgc acgggaacat catgcggaga tgccgccagt
     1801 gcgcatgaac aaaccggaat gtgaggttcc ccgggtaatg cccctgcgcc acgaacgtgc
     1861 cgtaatcgcg cgcgagtgtg agcgcctgat cgaacacctt cgtgttgccc gtggtttcga
     1921 acaccacgtc cgcgcctttt ttctgcacct tgcgaagttc ttcgtcaaac gccggaacgt
     1981 cgagggcaaa tgccctgtgg gcgctgtaac gggcggaaat ccccacgcgc gccgggtcaa
     2041 gatcgctcgt gaacaccgtg gcgccgttgt ggcgacagat ctgcgctaca agctgcccaa
     2101 gcacaccctg gccaaccacg gcgacaagat cgttgcgctg cacgtttgcc agccggatgc
     2161 cctcgaccgc cacgctcacc agaacgaaat acgaggccgc gtcgtccgac acgtgcgacg
     2221 ggacgatgat cgcccggtcg ttgcccggtt ccgccggaat caccgcgtgg gagaaatgcg
     2281 ccgccccggc cccctcgtgg tagccttccg gtatttctga cgacccgatg ctgatccggt
     2341 cccccggccg aagatgagag atgccttcgc cgacttcctc gaccaccccc acactctggt
     2401 agccgacaag gcacgggaac ttctggttcg gaaactgccc cgaaagcacg aggtattccg
     2461 tgccgatact taccccggac accacgaccc gagccagaac ttcgcctttt cgcggcgcgc
     2521 gaagaggaaa ttcgcgcagt tccgcccgat tcgcctcggg aaatacgacc gccgtgaatg
     2581 ttcgcgccat gtggttaccc ctttcaccag tccagtccaa ctttgaaggc ttccctgttc
     2641 gcaagaaggc tgaatccccg gccgatatcc cggatgggca atcggtggga aaggaacgcg
     2701 aaggcgtcaa tgcgaccttc ccgcacaagc gagaagaact cctcgtgcac ctcagattcg
     2761 tccgggctga tggctcgaac gcggggatga tttgcgtgga gagcctttgc ctgcgtgctg
     2821 ggtgcgacgc catacaatcc gatctggcct tcgggcgaca ggcacggaac cgccagatct
     2881 atcacactcg tgtcgccaac cccttcaatc gctcgatcaa caccctcgcc gttggtgatc
     2941 tcgtgaaccc gcgcgacgaa atgcgggtcg gtcacgttca cggaggtatc cgggtgacac
     3001 agctctgccg cacgccggat cgaggactcc gtgcggccgg caaggatcac ctgctttgcg
     3061 cccgccatgc gtgaaaacag cacgaaagcg gttcccaccg gccctgttcc gaacacgagc
     3121 acgcggtggc ctttgcccac ggcgaaccgt cgaatccagc tcagtgtctc cttcagtgtg
     3181 atggagagaa ccccgagcat ggggtcggtg cccgccggaa gcacctgctg cgggtggccg
     3241 aacgcacgag caccggtggg cggattggcg tcgtccattg cccactggtc ggtcacgtac
     3301 gcgaaatcgg caaacgagcc gaaatactcc ctgatgttga actcgggcgg gaagcatacc
     3361 cgcggccgaa tgaccagatc tccgacgcga tagttgcgca ccttcgcgcc aactgccgtc
     3421 acttcgccga ccgcttcatg gcccagcacc gccggaaatg cggtgcatcc gggaaaggtg
     3481 ccgtgcagga gcttcgcgtc cgtgctgttg cagatactgc ccgcaacgag ccgcgtccgg
     3541 gcgtggtagt ctcccatggg agcctcgggc acctcacgaa accccagttc gtgcgcccgt
     3601 tcgatcagga ctgcgttcat ctgcgtaact ccttttccgc ggtaacccct ccggttggag
     3661 cgtccggtac catagggtgt aatggtaccc tgtcagggag caaggagggt cagagaagag
     3721 cggggaatct ggctccggga agggttctgt tacgctgccg gcaggagccc gagtagcaat
     3781 gttggcacgt tcacgggcaa cacggaaaga caacggtccg caagaccaaa gtccgccatg
     3841 ttccgaacgc ttccccgtct ggtcgggaaa aaccggactg ggtcagacgg tacaaccagc
     3901 acggcaaggg acaacagacc gtttgccaac ccgtatcgga acttgtaaaa atcccggtaa
     3961 agtgccgccg cgttaccgaa ttgaacctcg acgaacacca gccctttgcg gaaatcaccg
     4021 atgagtttcg ggtccgtacg gagcacaggc tgcggatccc agcccatcgt tccgaattcc
     4081 gctgccagcg ccagattgta cgctgcctgg tgtcggtaca ctgtgcctcc gtgtgtgtat
     4141 tcgaaggaat ccctccactc cactgcgtgt accgcggcga gcacctcctc aatctccttg
     4201 tccatggttg caaggaggac gtctccgcag tgaaatagct ttttctgaac ctgcatcggt
     4261 ccacgattcc gcagaagcaa ggagtttagc aggtggctcg gggagtttcc ggggtgacca
     4321 cttttcgggt tttgagcacg tcgatactcc tccgccagta ctcaatctcc tgttcccgtg
     4381 tcagcccggc tgtctcccga tagatgcgct ccgcggccct gcgtttcatt gcaacgcagt
     4441 caaagcgttt ccgtttcgtc ttcattgcca acgatctcca gaggagaatg aatcatgatc
     4501 cccgggaacc gttcaaagcg ttcactgcat tgaaccgggg aattctcctg tagttcagaa
     4561 tagtttgaaa ttccagctcg cgatcatccc acaaccgctc actgtggcca gtgcgatatg
     4621 aagagcgtcg ttggatgcgg gaggcgggag aacacccgcc gacagttatg cgtcccgcaa
     4681 ggccaatgct tcctgcgtga cggggaccac ttctgccaga ggaagcattt tctcccccag
     4741 cgctcgcact ctgggcggtg caccggccag ttcgcgcacg acaacgtcag acacaaccat
     4801 cacaaacgcc cgcctgtgca tgtgttcgaa aaacacgctg ctccgttctc gaaactcgtc
     4861 atcgaacacg gcccccgaaa acggaggttt cgacgtacac gcgcaacagt gtcgttcgca
     4921 tcggattccc ccgacagcaa cattgtgagg ctaaagtgaa aatacgaatt ccgttgtgga
     4981 ttcgtacatt catggtgatg tgtgtgttcg tgtcgtatct gtcgcggcaa aacctgtggt
     5041 ggaacggcac ttgaatggca aaggggccgc ctcatgtcct catggtttcc ccggggagag
     5101 ttccccgtac ccgataaatc ggttcagaaa gcccttgcac acgcagaaag ggcaatcaat
     5161 gggattgcga agggcacgga gggtgttccg tgtgcatggg gtcgcgttca caacggcggc
     5221 aatgcaggtt tcagtgtgca cgccatgagg aggaccgcca cccgcctgcc gtggatggtt
     5281 gttcatttcg cgttccacga gcatccggcg ggagtggtct gctcgtatcg gacacgatac
     5341 tccgtgatgt cgctctttct tttgctcggt gtctctgtgg tgttcctcgc cttccccctg
     5401 caggttgccg gaacctccgt gttcgggtgg cacctgtcgt ttgcgtggtg ggagtacgtg
     5461 gttgctgtcc tctcgcccct cgtgtggctg tggctggttc aggacggacg taacgacgca
     5521 cgcagggtca tcactgagtt ccagcggcgc ctgggagaga acttcgccgc gggttgaggg
     5581 cgcagtttcg agtcagccgc gcggaccgga gtgcggggag agctcgttca gcgccgttct
     5641 ctcctggagc gcagggcgcg ttccagtgct tccgtcgacg ccgcaatcat tacgtgttcc
     5701 gacacgtacg ccgatccctg aatataaaac tccgcaagct ggttttcgga cgcggcaaga
     5761 tagatgtagg gaatcgtggc aggctgggtc gccacgcgat tctggaacaa cgtcatcacc
     5821 cgtccgattg cggatatcgg cgggaacgac atcccgcgat ccacatacac acgcgtcccg
     5881 ccgacacgct ggacatccca caggtgggtg agaatgcgga actccaccaa ttcggacggc
     5941 tccagcacct cctcgttctg gcggacgacc cacaccctga aatcacgttc cagagcggga
     6001 aatctcacgc catatacccg ttccagcacg ggtatgaaat ccgtgtcgcg gtgaatggaa
     6061 cgcgcggcag accggaactt cgaagcgcca tactcgctga caagaaaccg gacgaatgcg
     6121 cccgcctctg ctaccacaag gggattatcg gggctggaga gcgcgtcgcg aaggggaatg
     6181 tagcgcccct gcgaaatcaa tgtgcgaacc atgaaaacgg ccgatttccc ccttacatat
     6241 ccgccgttcc cgtctccgaa cgtcgaaaca ccgttgataa aaaggcggca taaccgatcg
     6301 tttcccgtac tattcagctt accgagcgaa tcccctgaca cctgaaacag gagccgcgtt
     6361 gttacgagct gaaaaacccc gtgggggaaa tccgacacga tggcgccggt ttccggctcg
     6421 atgaacgctt cgtcggccgt agagccgaaa aggctcatca tctccgcctt ggtgttcacc
     6481 aggtagatgt atatccgctc cgtggaggcc gggttcagga ggttcgccat tgcggcccgg
     6541 tgccgttcaa gcagggttgc cgccatgttg gtcggaacgg ctacccctct tccgatggaa
     6601 tccttgtacg ggttgtagac aaacgtgaaa tgaggagacc gccgttccac ccagtgctgg
     6661 gagtacgcgt gccagcggta ggtaagaact accttaccac tctctctgac gaagtagaac
     6721 gagtgatacg aacgccggaa attctccgtg acttcaaggt tcaaccgcgc gaatgcggga
     6781 tacacgtcca tccgcagtac gcggaaccgc tgcacggagc caagcgcaac cagtgaggtg
     6841 acaagccgcg tgtttctgta gcgctccgca acatacgggc ggacaagccc cgggctgtgt
     6901 tgccgggcac cggacaacac cgcgttccag gtgcgttcca cggcggcgga atcaagaggc
     6961 gcaacggagg aatcgggggg agcgtcttcc tcgaacgagt atacctgcgc cgcgctccag
     7021 gccggaagca tacacagaag ggcaaatacc cggatcaggc cacacggcat cggatctatc
     7081 tgctcgcacg aagtacggta cgattggagt tcgcaacgcc cggttgcgcg tactcaaaaa
     7141 tatacacacc cccccgttcc accgcgaggt ggcaccatgc cgtccggttc cgattttttc
     7201 cgacgaacgg tacattcaga ccacgcatac tctctcgcac cgttgccttc gcaaggagcc
     7261 ccggcatgac accgcgtgaa atcgttcaga tgagcctcgc gttcgcatat cctccgcgca
     7321 tcccgtttgg aatgggcagc gccggttttc cgagtgactt cagaggcgtg caccacgatc
     7381 ctgcacccaa tcgcgttcag cagccgtgga cccagcgcga cgggtactgg gacatgatcg
     7441 acatgtgggg aaacacgtgg cgccgtctcg aaagcatcac caaaggcgag gtgtacaagg
     7501 gcgtcattca ggatagctgg gaccttcttg acacgtacga gttcccgatt ctcgacaggg
     7561 ccgaactttt cgaaaaggcc gccgccgaag tcaagcaata ccatgcggaa gggtattacg
     7621 ttctcggtgg tatgagctgg accttcgaca ccgcgcgata catgcggcgt atggaggtgt
     7681 tccttcgcga ctgcgcggaa gagcctgacc gtgtgtccta ccttctgaac cgtatcgccg
     7741 acgtgtacga acgggtgatc gagcggtacg ccgacattgg cgtggatggc atctctaccg
     7801 gcgaagactg gggcacgcag gatcgccttc ttgtcagccc gcccatgttc cgccggctgt
     7861 tcaagcccgt attcgcccgc ctgtgcggag tggcgaaaag ccgcggtctg agcatccatt
     7921 tccactcatg cggtttcgtg cgcgacgtaa taggcgactg gatcgaggtc gggattgatt
     7981 ccttcaagtt cgatcagccg gagcttcacg gtattgactg gctggccgag cactacggcg
     8041 ggaaaatgca catctggtcg cccgtggaca ttcagacgac cctccagacc cgcgaccacg
     8101 cccgcattga agcggcggcg cgggaataca tcgagaaact ggcgctaccc ttcgggggag
     8161 gcgtcatcgc cgggtactac ggctccaacg aagcaatcgg gctcacgccg gagtatcagg
     8221 agacggcttc cagggcgttc atgaaatacg gcgatcccgc gttgtggacg gggcgcccgg
     8281 ccagcagaac ggaaaaggcg tagttcccat gcgcgcggtc gttcagcggg tcagttcggc
     8341 acacgttgcc gtggatggtc agaccgtcgg cgccatcggg cccggacttg tcgtgctttt
     8401 gggcgtggcc gagggcgact ccgacgcgga tgtggcctac atggccgaga aaatcgcaaa
     8461 cctgcggatt tttgaggacg ccgccggcaa aatgaaccgg tcgctgctcg aaaccggagg
     8521 ggcgctgttg gcggtgtcgc agttcacgct cctgggggat tgccgcaaag gtcggcgccc
     8581 gtccttcatc gaagccgcgc ggcccgagga gggtaagcga ctgtacgacg cattttgcga
     8641 cgccgtgcgg gcgttcgggg ttcgcgtgga aaccggccgc tttcaggcca tgatgcaggt
     8701 ggctcttgtg aacgatggcc ccgtgacgat tctcgtggac agcaggaagg ttttttgacg
     8761 cggcaacgcg cccctgtctg aatatgcacc ggaccgcggg ggaacaaagt agagcgcccc
     8821 cttgaaacgt aggggcggat ctctgtgtcc gcccccgccg gcacacacgt tatgatcacg
     8881 ggtggacaca cgggccgaca cgggggtcgg cccctgcatc ttggcgcccg taccctcact
     8941 tacctcacca gcaccatcct cctcgtcacc actccctgcg ccgccgtgag gctgtataca
     9001 tacaccccgc tggcaaccgc gcgacccata gcgtcatatc cgtcccatac aacctcgtgg
     9061 tggccaggca ggaatgcctg gcccaccaac gtccgcacga ggcggccgtt gatgtcatac
     9121 accgccagcg tcacgtgccc cgcctccg
//
LOCUS       DAOSOO010000123         9082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7615_length_9082_cov_7.096378, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000123 DAOSOO010000000
VERSION     DAOSOO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7615_length_9082_cov_7.096378"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /gene="ribH"
                     /locus_tag="PLT86_09405"
     CDS             <1..314
                     /gene="ribH"
                     /locus_tag="PLT86_09405"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT36869.1"
                     /translation="GSFEIGVTARRLAKTNAYNAIICLGAVIRGSTPHFDYVANEAAK
                     AIAQVGLETGVPTIFGVLTTDSVEQAIERAGTKMGNKGADAALAAIEMASLFACLPTS
                     E"
     gene            293..742
                     /gene="nusB"
                     /locus_tag="PLT86_09410"
     CDS             293..742
                     /gene="nusB"
                     /locus_tag="PLT86_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT36870.1"
                     /translation="MSADLGVKRESRHAARKIAVQTLYWLQSQPWDNVSDIVQNLGDE
                     EHVAEQSRERALLLCRHAEEKRPEFERLAASACENWEIERVGRVEQVIIRLALAEWGL
                     KDVPPRAVLSEAVNLAREFCGDRAAGFVNGVLDRIARETGVLAPPDK"
     gene            1025..4036
                     /locus_tag="PLT86_09415"
     CDS             1025..4036
                     /locus_tag="PLT86_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36871.1"
                     /translation="MPRLPASISRLVFVIIALSLAGPSLFAQTPVSRREADDYTFARR
                     LYDQGDYDLAERQLRDFLTRYPASPRRADVRFMLADCDFNRGRYATALEQFERFRIEF
                     GASPHDDDALFRIGQCLFRLARYQQAADAFSRLLLAYPSSNLRYEAEMFLGEARFELG
                     QLEAAARSYEQAAQGTGKVVAHALYSLGWVRQKQGNYEEAISAYQRMLQNDPSSALAS
                     QARFKLGECLYQQKRYQEAVSALSQALPRLSDPQLRANANYLLGESYRALGQYARART
                     YYQTITSEYRTTSLAPHSQYAIGLTHVEERNLQEAQRAFAEVTAKFPESAIVPNAEFR
                     RAIVLKDLGNRSEAERIFSLVATRYPSSDVADDALYELGVLAYNRMAYEEAERSFRRI
                     TYDYPGSDLRPQATYMMGEIALARGTSDSAEVYYSRFIGSYPTHSLLPNAFFQRGSTR
                     FKQGRYQLAIDDLTQVVRRWTSHALVPDAYFLVAESRYALGDYAGAAANYQLVIDRFA
                     QSPLVAQAYYGLGWCAFKTRDWAKAARAFERAKTLATDPRIKSDAAVRIGDSYYNQAN
                     FTRALAAYREASEINNSDPRAVYYAAQSLFQTGRLREAISLYEQVTRRFPESDLADDA
                     WYMIGWSRLRMDDYEGAISAYRHLVLHYPSSEKAPEAQLGLGDAYYNSGRFSDAIAAY
                     DDVLRRWPNSEQVQGAVTGLWDSYLRQGKIDEAIERTERFIQANPRKPASASLLMKRA
                     DWFYSAGRYEDAVQAYRRIADEFEGVSLPTSPVFWEASALAQLGRRDQAIARFRVVSL
                     EPDNPLAPDALERIGDLQREAGALQAAVSSYQEYERRFPNSSKRLAVLAKLGDAQRDL
                     GNEAAAEQAYRTAAQAGPASAAGAQASLSLAELLLKRGGYDQARRLADSVLVSRSDES
                     AARAQFILGEIAFAQRKFEDARLAYMRVKLIYSSFPDWVARAQIRIADCYREMGNRAA
                     ARDSYREVLNTHPSDSLGIRARRQLDLLGGR"
     gene            4039..5721
                     /locus_tag="PLT86_09420"
     CDS             4039..5721
                     /locus_tag="PLT86_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36872.1"
                     /translation="MARGYARSAGAFASILVVLAGLPNLATGQAPETPVLDIPDSLVI
                     QGRVQHELLLAGTKSNTLYDRAFPVEREDVPEQLASVNTLPAISSKETFPLFIRHGSV
                     HATALRAAAQHFGNGSLWVGRWQDWKGFLLSSDLELGGGRGHTDNASWWRTALGTNTR
                     YMPSWRTACDASLDIESGRTAIWDGVLSSHRNYSRGGLGLALNRGLSPDQEVSLFVEG
                     DARRVSGPEATANEKTLASVISWQNTTGRFWIKGNVQADFTSTARPAGTDGTGSFYAV
                     SAEAWTRPEENFGGAVGVTIYTVNYLSGDSLRTIRPSIAAWGRVRNLVKFTGRLSSGV
                     QRFGIWEAYQANQMLNLATPLRSPFTSVDIDLNVETSVHRDNVLTFGFRQLIVDDYAV
                     WVRKDSTVTWRRRFPTEPHTVNQFGVDYGYAGTDQASLTSFYARYQYAWREGSVDALG
                     LIRAHSLVQQPVPYVPDWEFHLGARFPFRRFTVAPSLHTIGTRHYIEPKTLDQIAEMD
                     PYVLVDLEVSTALNRHWQLALLGSNLLNSRYQRWNGFNEPGIYGQIGIRRTW"
     gene            5788..6393
                     /locus_tag="PLT86_09425"
     CDS             5788..6393
                     /locus_tag="PLT86_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT36873.1"
                     /translation="MTLLSLAIQGGWLMIPIALCSIIGVWVYVERYLTYRRVRLDGIS
                     LLEQIRPAVTSRQTAEAERRLAMNPAPVGQVIAAGLRSLAGGRDAVREAMENAGNRQI
                     HLLEKRLSILATVAGVAPMLGFLGTVTGMIIAFQQIENLGGNVNATVLAGGIWQAMIT
                     TAFGLIVGIPALIGYNHLSGIVQEFAADIEHAGAEFLTLVN"
     gene            6410..6808
                     /locus_tag="PLT86_09430"
     CDS             6410..6808
                     /locus_tag="PLT86_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT36874.1"
                     /translation="MKLRKPQGAITTFAAQSLTDIVFLLLIFFLLTSTFVLQTGVKVQ
                     LPQTTADEPVSEKMVVISLTKDGSVYLNENLVSRPELVGRLRQLLVSKEQIVILRADK
                     SLSLDLVVGVMDIAKSSGASRFLIATQTEE"
     gene            6814..7587
                     /locus_tag="PLT86_09435"
     CDS             6814..7587
                     /locus_tag="PLT86_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36875.1"
                     /translation="MRASWTETPQARNRTAAIVSFGLHGLLAAAAAFFAITAATPPPE
                     FVELNVGRLSQAELNRLIQEGERASAAASPEERAQTPQQRLPDIEMPTISPTEAERRL
                     MPTQVALKENKVFVAPPRPTAIAAPPIPVQSIDRKVRYEGGQISLGPRPGEGIVSEHV
                     GSDIHPVFQIEGDLIGRRFYEAAITETPNVPAQTRIQFDLVVSPSGAVISALVVRREN
                     SDLEAFAANYLRRCRFDPLPAGMPQENQTGRITITFSAG"
     gene            7609..8130
                     /locus_tag="PLT86_09440"
     CDS             7609..8130
                     /locus_tag="PLT86_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT36876.1"
                     /translation="MAHAVWANPKSLFRPRTPLAKALGDLPLFSRLSRAEIEKVEAIA
                     HIRTYSAGECIFEQGDPSVAMFVIMAGEVRIVIRTEESEEDREVARLGTGEMFGETAL
                     LDGAPRTAAAIAATPAEIAAIARPDWIDLVERQPALGVRMLLPLARVMAARVRALNLQ
                     VPTSQQQNWEQYV"
     gene            8127..>9082
                     /locus_tag="PLT86_09445"
     CDS             8127..>9082
                     /locus_tag="PLT86_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT36877.1"
                     /translation="MNTGSSAAKFGWIRLTALGVGCAIGLYVIRDVLPPIVLAAIMAY
                     ILNPIVDMGERRSVPRWVTIILIYLLITAAAVVGVLFLLPLLGQQFEEIRSQIGPFWS
                     QMQVYGYRFLDRIERWWPPLARVILAEVTSDALTTMPHQWLASALENAPAFISGAFSN
                     VVGIITYFITVPFIAFFLVRDGRAFKRAFIELVPNRYFETVVSVLDRIDHSVGLYIRG
                     ILLEALLLGTLATIGLLLIGLKGAILIGMLTGFLNLIPYVGAIVGFVTGVFVALATGG
                     NVPGVVIVFAFSQFVDNWFIQPFVLSRSVNLHPLLIFLAVVFG"
BASE COUNT         1733 a         2760 c         2776 g         1813 t
ORIGIN      
        1 ccggttcatt cgaaatcggt gtcacggcgc gacgtttggc gaaaacgaac gcgtataacg
       61 ccataatctg tctcggagcc gtcatccggg gctccacgcc gcatttcgac tacgttgcca
      121 acgaggcggc aaaagcgatc gcgcaggtgg gtctggaaac cggcgttccg acgatctttg
      181 gcgttctcac taccgactcg gtcgagcagg cgattgagcg cgcaggcacg aaaatgggga
      241 ataagggtgc cgatgcggcc ctcgcagcaa tcgaaatggc aagcctgttc gcatgtctgc
      301 cgacctcgga gtaaagcggg aaagccgcca cgcggcacga aagatcgcgg tgcaaaccct
      361 gtactggctc cagagccagc cgtgggacaa cgtcagcgac atcgtccaga acctcggtga
      421 cgaggaacac gtcgcggagc aatcacgcga acgggcgctc ctgctgtgcc gtcacgcgga
      481 ggagaagcgc cccgaatttg aacgcctggc ggcttccgcc tgcgaaaact gggaaataga
      541 acgcgtcgga cgggtggaac aggtaatcat ccgactcgcg ctggcggaat ggggtctgaa
      601 agacgttccc cccagagccg tcctgagcga ggccgtcaat ctggcacggg aattctgcgg
      661 agacagggcc gcaggatttg tgaacggtgt gctcgatcgc atcgcccggg agacaggcgt
      721 gcttgccccg cccgacaaat gacccattac cggacgcgat gatcggtaca cggcgtggaa
      781 caaagacgtg caacctgcgt atgcagattg cacgttcttg ttgtcgggcc gtcccggccg
      841 ttcgggatgg aagccgcatg cgcgcaattt ccccggtgga gtttcgacgc gagccctgtc
      901 tggattcgcc acggagacgt tgcactcaat tgccctaagt tggctcgaag tttgcacctg
      961 acgtatcatt gttgaataca gcagtcctta tccgcgatca ggacacggag cgaaccgacc
     1021 gcaaatgcct cgccttcctg cctcgatttc ccgtctggtg ttcgtcatca tcgctctgtc
     1081 gcttgccggg ccttcgttgt tcgcgcagac gccggtcagc aggagagaag ccgacgatta
     1141 caccttcgcg cgccgcctgt atgaccaggg cgattacgac ctcgctgaac ggcagttgcg
     1201 cgactttctg acgcgatacc ccgccagtcc gcgccgggca gatgtccggt tcatgctcgc
     1261 ggattgcgac ttcaatcgcg gacgatatgc cactgcgttg gagcagtttg aacgcttccg
     1321 catcgagttc ggcgcatctc cccacgacga cgatgcgctg ttcaggattg gccagtgtct
     1381 tttccgtctc gcgcggtacc agcaggctgc cgacgccttc agccggttgt tgctggcata
     1441 cccgtcatcc aatctccgct acgaagcaga gatgttcctg ggcgaagcgc gctttgaact
     1501 cgggcagctt gaggcggcgg cgcggagtta cgaacaggcg gcgcaaggca ccgggaaagt
     1561 ggttgctcac gcgctgtaca gcctcgggtg ggtgcgccag aaacagggaa actacgaaga
     1621 agcaatttcc gcataccagc gaatgcttca aaacgatccc tcaagcgcgc tggcctcgca
     1681 ggccaggttc aaactcggcg agtgcctgta ccaacagaaa cggtatcagg aggcagtcag
     1741 cgccctctcg caggcgcttc caaggctctc agatccccag cttcgcgcca acgccaacta
     1801 cctcctcggc gaaagctacc gtgcgctggg ccagtatgcc cgtgcgagaa cctattacca
     1861 gaccattacg tcagaatacc gcaccacctc cctcgcgccg cactcccagt atgccatcgg
     1921 cctcacgcac gtcgaggaac gaaaccttca ggaggcgcag cgagcgttcg cggaagttac
     1981 tgcgaagttc cccgaatcgg cgatcgttcc caacgcggag tttcgcagag cgatcgtgtt
     2041 gaaggacctg ggcaatcgct ccgaggcaga acggattttc tccctcgtgg cgacgcgtta
     2101 tccgtcttct gacgtcgcgg acgacgctct ctacgaactc ggtgtgcttg cgtacaaccg
     2161 aatggcatac gaggaagccg aacggtcgtt ccgcaggatc acgtacgact accccggcag
     2221 cgaccttcgt ccgcaggcga cgtacatgat gggagaaata gcgctggctc gaggcacctc
     2281 cgacagcgcg gaagtgtatt actcccggtt catcgggtcg tacccgaccc attccctgct
     2341 cccgaacgcc tttttccagc gcggctcgac ccgcttcaaa cagggtcggt atcagcttgc
     2401 cattgatgac ctcactcagg tggtgcgccg gtggacctcc catgcgctgg ttcccgatgc
     2461 gtatttcctc gtcgccgaat cccggtacgc gctcggggat tacgccggcg ccgcggcaaa
     2521 ttaccagctc gtgatcgacc gctttgccca aagccccctt gtcgcgcagg catactacgg
     2581 tcttggctgg tgcgcgttca aaacccgcga ctgggcgaaa gccgcgcgcg cgttcgagag
     2641 ggcgaaaaca ctcgccacag accctcgaat caaatccgac gccgcggtgc ggatcggcga
     2701 ctcctactac aatcaggcga acttcacccg cgctcttgcg gcctatcgtg aggcgagcga
     2761 gatcaataat tcggacccca gagccgtcta ttacgccgcc cagagccttt tccagaccgg
     2821 acgtctccgc gaggccattt ctctgtacga gcaggtgacc cggagattcc cggaatccga
     2881 tcttgccgac gatgcctggt acatgatcgg atggtcacgg ctgcggatgg atgactatga
     2941 gggtgccatc agcgcctatc gccacctcgt gctgcactac ccgtcgagcg agaaggcccc
     3001 ggaggcgcaa ctgggcctcg gcgacgccta ctacaacagc gggcgattca gcgacgcgat
     3061 cgccgcttac gacgatgtgt tgcggcggtg gccgaactct gaacaggtgc agggcgccgt
     3121 cacggggctg tgggattcct acctgcgcca ggggaagatc gacgaggcaa tcgagcgcac
     3181 ggagcggttc atccaggcca atccccggaa acccgcgtct gccagccttc tcatgaagcg
     3241 cgccgactgg ttctactccg cgggcaggta tgaagacgcg gtacaggcct atcgtagaat
     3301 cgccgatgag ttcgaaggcg tgtcattgcc aacctcgccg gtcttctggg aagccagtgc
     3361 gctggcccag ttgggaagac gtgatcaggc gatcgctcgc ttccgggtgg tctcgctgga
     3421 gcctgacaat cctctcgctc cggatgctct ggaacggatc ggcgacctgc agcgcgaggc
     3481 tggtgcgcta caggcggcag tgtcgagcta tcaggagtac gagcgccgct tccccaacag
     3541 ctctaagcgg ctggccgtgc tggccaaact cggcgatgcc cagagagatc tcggcaatga
     3601 ggccgccgcg gagcaggcat accgcactgc agcgcaggcc ggacctgcct ccgccgccgg
     3661 ggcgcaggct tccctctcgc tggcagagct gttgctgaag cgcggagggt acgatcaggc
     3721 acgccgactc gccgattccg tcctcgtgtc ccggtcagac gagagtgcgg cgcgggcgca
     3781 gttcattctc ggcgagatcg catttgccca gcgcaaattt gaggacgcgc gcctcgcgta
     3841 catgcgcgtc aaactcattt actccagctt tcccgactgg gtggcccggg cacagatccg
     3901 catcgccgac tgctaccgcg agatggggaa ccgggcggcg gcacgcgatt cgtaccgcga
     3961 ggtgctgaac actcatcctt cggatagtct cggcatccgc gcacggcgcc agctcgatct
     4021 gctcggagga cgctgaacat ggcgcggggg tatgcgcgga gtgcgggagc gttcgcgtca
     4081 attttggttg tcctcgcggg cctgccgaat ctggcaaccg gacaggcgcc ggagacgccc
     4141 gtgctggata tccctgattc cctggttatt cagggacgtg tccagcacga gctcctcctc
     4201 gcgggaacga aaagcaacac gctgtatgac cgcgctttcc cggtggaacg ggaggatgtt
     4261 ccggaacaac tcgcgtcggt aaacacgctg cccgcgattt catccaagga gaccttccct
     4321 ctcttcatac gccacggctc cgtgcacgcg acggcgctca gagcagcggc acagcatttc
     4381 gggaacggtt ccctgtgggt gggccgctgg caggattgga aaggtttcct tctttccagt
     4441 gatctggagc ttggcggcgg acgcggccac actgacaacg cctcctggtg gcgcaccgct
     4501 ttgggcacga acacccgtta tatgccctcc tggcgcaccg cctgcgatgc ctccctcgac
     4561 attgagtccg gccgaaccgc aatctgggac ggcgtgttgt cctctcaccg gaattactct
     4621 cggggcgggc tggggctcgc actcaaccgc ggcctctccc ccgatcagga ggtaagcctc
     4681 ttcgtagagg gcgatgcgcg ccgcgtcagc ggtcctgaag cgacagcgaa tgaaaaaacg
     4741 ttggcctccg tgatttcgtg gcagaacacc accggccgct tctggataaa agggaacgtt
     4801 caggcggact tcacaagcac cgcgcgcccg gcaggcacgg acggcaccgg ttcattctac
     4861 gcggtaagtg cggaggcgtg gactcgcccc gaagagaact tcggcggtgc ggtcggcgtc
     4921 accatctaca ccgtcaacta cctgtccggc gattccctgc ggacgatccg cccgtcgatt
     4981 gctgcgtggg gccgggtacg aaatctcgta aaattcaccg ggcgtctttc ttccggcgtg
     5041 caacggtttg gaatctggga agcctatcag gccaatcaga tgctgaacct cgccacgccc
     5101 ctgcggtcgc ccttcacaag cgtggatatc gatctcaatg tagaaacatc cgtccaccgg
     5161 gataacgttc ttacgttcgg gttcaggcag ttgattgtcg acgattacgc ggtctgggtc
     5221 cggaaggaca gtaccgtcac gtggcggcgg cggttcccga cggaacccca caccgtgaac
     5281 cagttcggtg tggactatgg ctacgcaggc accgatcagg cgtcgctcac gtcgttctat
     5341 gcgcggtatc agtacgcctg gcgcgaagga tccgtggatg cgctcggact gattcgggcg
     5401 cactctctcg tgcagcagcc tgttccgtat gttccggact gggagtttca cctcggggcg
     5461 cgtttcccgt tccgacgttt caccgtcgcg ccctcgctgc acaccattgg aacccgacat
     5521 tacattgaac cgaaaacgtt ggaccagatt gcggaaatgg acccctacgt ccttgttgat
     5581 ctcgaagtat ctaccgcgct gaaccgccac tggcagctgg ccctgctcgg atcgaacctt
     5641 ctcaacagca gataccagcg ctggaatggg ttcaatgaac cgggtattta cggccagatt
     5701 ggcattcgac gtacatggta gaatgtgcgg agtggatttt gtaacgagtt ccttaacgga
     5761 gccggtttgt aaccaaggag tacggagatg acgttgttgt cgctggcgat tcagggcgga
     5821 tggttgatga tcccgattgc gctctgttca attatcggtg tctgggtgta cgtggaacgc
     5881 tacctgacgt accggcgggt tcgactggac ggcatctcgc tcctggagca aattcgaccg
     5941 gcggtcactt cgcggcagac agcggaggcg gaacgccgtc tcgcgatgaa tcccgcgcct
     6001 gtcggtcagg tgattgcggc cggactgcgg agcctggcag gtgggcggga tgccgtccgg
     6061 gaggcgatgg agaacgccgg caacaggcag atccatctcc tggagaaacg cctttccata
     6121 cttgccacgg tcgccggagt tgcacccatg ctgggctttc tcggcacggt aaccggtatg
     6181 attatcgcct tccagcagat cgaaaacctt ggcggaaacg tcaacgcgac ggttctggcg
     6241 ggaggtatct ggcaggcaat gattaccacc gcattcggcc ttatcgtcgg cattcccgcg
     6301 cttatcggat acaaccacct gagtggaatc gtacaggagt ttgccgctga catcgagcat
     6361 gccggtgccg agtttcttac cctggtgaac tgacgggagc gctgcgatta tgaaacttcg
     6421 caaaccacag ggcgcgatca ccacgttcgc cgcgcagtct ctgacggata tcgtgtttct
     6481 cctgctcatt ttcttcctgc ttacctccac gttcgttctc cagaccggcg tgaaggtgca
     6541 attgcctcag acgaccgcgg acgaacccgt gtcggagaag atggtcgtga tttctctcac
     6601 gaaggacggc tccgtgtacc tgaacgagaa cctcgtcagc cgcccggaat tggtgggaag
     6661 gcttcggcag ttgctggtga gcaaagaaca aatcgtgatc cttcgtgcgg acaaaagcct
     6721 ctcactggat cttgtggtgg gagttatgga tatcgcgaaa tcgtcgggag cgtcgcgctt
     6781 cctgatcgca acgcagaccg aggagtaggt cggatgcgcg catcgtggac agaaacgccg
     6841 caggcgagaa accgtaccgc ggcgatagtg tccttcggct tgcacgggtt gttggctgct
     6901 gcggccgcat ttttcgcgat taccgcggca actccgccgc ccgagttcgt cgagttgaac
     6961 gtggggcggt tgtctcaggc ggagctcaac agactgattc aggagggcga gcgcgcgtcc
     7021 gctgctgcct cgccggagga acgggcacag acgccgcagc aacgtctccc tgatatcgaa
     7081 atgcccacga tcagcccgac ggaggcggag cgtcgcctta tgccgacgca ggtcgcgctg
     7141 aaagagaaca aggtgttcgt ggcgccgccg cgcccgactg cgatcgccgc ccctccaatc
     7201 ccggtgcaga gcatcgatcg gaaagttcgc tacgagggcg gacagatcag tctggggccc
     7261 aggccgggtg agggaattgt gtccgaacac gtgggaagcg acattcaccc tgtgttccag
     7321 attgagggag acctgatagg aaggcggttc tacgaggccg cgatcacgga aacccccaat
     7381 gtgccggcgc agacccgtat ccagttcgac ctggtcgttt cgccgtccgg ggcagtaatc
     7441 agcgcgctcg tcgttcgacg cgaaaactcc gatctggaag cattcgctgc caattacctg
     7501 cgccgctgcc ggttcgatcc gttgcccgcg gggatgccgc aggaaaacca gaccggccga
     7561 atcaccatca cgttctctgc aggttgaatc tgcctgcagg aggaatacat ggcgcatgcc
     7621 gtgtgggcaa atccaaaatc gctcttccga ccgcgcacgc cactcgcgaa ggccctgggt
     7681 gaccttcccc tgttctcccg cctttcccgc gcggaaatcg aaaaggtgga ggccatcgca
     7741 cacatccgta cctacagcgc gggcgagtgc attttcgaac agggcgaccc gagcgtggcc
     7801 atgttcgtca taatggcggg tgaagtgcgt atcgtcatcc ggactgagga atccgaggaa
     7861 gaccgggagg ttgcgcgtct cggcacggga gaaatgttcg gggaaaccgc gctgctcgac
     7921 ggtgcgccgc ggactgcggc cgccatcgcg gccacaccgg cggaaattgc cgcaattgca
     7981 cggccggact ggatcgacct cgtcgaacgg cagccggctc ttggtgttcg catgttgctt
     8041 ccgcttgcgc gggttatggc ggcaagggtt cgcgcgctga acctgcaggt gccgacctcg
     8101 caacaacaga actgggagca atacgtatga acaccggcag ctcagcggcg aaattcgggt
     8161 ggatccggct gacggctctc ggtgtggggt gcgcgatcgg gttgtacgtg atccgcgatg
     8221 tgttgccgcc gatcgttctg gcagccatca tggcgtacat cctcaacccg atcgtggata
     8281 tgggcgagcg caggtctgtg ccccggtggg tcacaatcat cctgatctat ctcctgatca
     8341 ccgctgcggc agtggttggc gtcctgttcc tgttgccgtt gcttggccag cagtttgagg
     8401 agattcgctc tcagatcggt cctttctggt cgcaaatgca ggtatatggc taccgtttcc
     8461 tcgaccggat cgagaggtgg tggcccccgc tggcgcgcgt gattctggcc gaagtcacca
     8521 gcgatgcact gacgacgatg ccacaccagt ggctggcaag cgcactggaa aacgcccccg
     8581 cgttcatttc cggagcattc tcaaacgttg tcggcattat cacttatttc attacggtgc
     8641 ctttcattgc cttttttctt gtgcgggatg gccgcgcatt caaacgggcg ttcatcgagc
     8701 tcgttcccaa ccggtacttc gaaaccgtcg tcagcgtgct tgacagaatt gaccactcag
     8761 tcgggcttta catccgcggt attcttctcg aggcacttct cctcggtaca ctggcgacca
     8821 tcggactgct cctcataggc ctcaagggag cgattctgat cgggatgctg accgggtttt
     8881 tgaacctcat tccctacgtg ggcgcgatcg tcggtttcgt cacgggggta tttgtagcgc
     8941 tcgcaacggg tggtaacgtt cccggggtgg tgatcgtgtt cgcattcagc cagtttgtgg
     9001 ataactggtt cattcagcct tttgtgttgt cacgctcggt aaacctgcac cctctcctca
     9061 tctttctcgc ggtggtattt gg
//
LOCUS       DAOSOO010000124         9062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7634_length_9062_cov_4.610858, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000124 DAOSOO010000000
VERSION     DAOSOO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_9062_cov_4.610858"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="gltX"
                     /locus_tag="PLT86_09450"
     CDS             <1..1377
                     /gene="gltX"
                     /locus_tag="PLT86_09450"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT36878.1"
                     /translation="FILRIEDTDQTRVVEGAIEAIFAGLRWLGLEWDEGPDVGGPVGP
                     YVQSCRLDIYRQHVDNLIASGHAYRCFCSEERLAELREAQRLAKKSPGYDRRCRNLSA
                     EEIRTALDSHTPFVVRMKIPLTGETRFEDRIRGTVQYDNKVLDDFVLFKSDGYPTYHL
                     ANVVDDHLMGITHVIRGEEWIPSTPKHVLLYDAFRWADSLPEFVHVPSILSPDGGKLS
                     KRKGAAAVTDFAEMGYLPEAVRNFIALLGWSPGGDREKMTLDEMIEAFALDRVNPKGA
                     IFDESKLEWLNGEYMKDMAAVDLLPRVAPLWIEKGLIAENDLENDREYLCHVIELFKG
                     RTRRTHDFVDLAGFCLRAPTSYDEKARAKHWSDPAAADRLAAAADAFANLEPFDARTT
                     EEVVRLLSEKEGVSASRYIHPIRLAVSGMSFGPGLFEMLEVLGKGRVVERLRTAVHVI
                     RSDRVGTD"
     gene            1463..1811
                     /gene="ssrA"
                     /locus_tag="PLT86_09455"
     tmRNA           1463..1811
                     /gene="ssrA"
                     /locus_tag="PLT86_09455"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1977..2819
                     /locus_tag="PLT86_09460"
     CDS             1977..2819
                     /locus_tag="PLT86_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT36879.1"
                     /translation="MTAKIASIWEMHYQWILDFGYKVLLTVVVLAASYLAAKAIRRSI
                     TRANERFERLDATLVPVLTALATYAVYALGTIIVLDILGVNTASIITVLGATALAIGL
                     ALKDTLGNLAAGIMLLILRPFRAGDLIKCGTIEGTVEEAHLFTTKLQTIDGLFVEAPN
                     SELWNNHITNFTRNGKRRMDIVVGISYSDPIDKGLEVLAQLVREEPRFLNEPPPQSMV
                     KALSDSCVEIQLRAWTSIDDYWNTYFDQNRRVKLAIEAAGLTIPFPQRDVHVVSGNMP
                     GPSQ"
     gene            2926..5046
                     /locus_tag="PLT86_09465"
     CDS             2926..5046
                     /locus_tag="PLT86_09465"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOT36880.1"
                     /translation="MVLDNGVLHLEFDDATGLLSHLIDVQGRVVHLAPPDEPLGIWKL
                     VFRAGTDEQSLVWPPWKGEVEMSCVRESDGTQVASIEWRDMWFRHEPKAVDVAVTVRL
                     PENSGTSEWRIRVSNRSARYGLWEVRFPWFNRWPAPKEYNVAIPWHNWGHRYQKVAER
                     HTGEYSSYTWSMQFLTFESGQRGLTIAALDPEQHYKSYLVDPGNETAFVLRVPDGSKP
                     GVGLDDTYATSISVHGDGWFGGCKLYREWALGQKWCAKGPISRRKDVPKTLKDIGIWL
                     LSSIPEKNSGVTPAEWADGVIAAQRYFDVPVGIQIYQWHEIKFDNDYPEYFPPKPGVP
                     EAVRKLVDAGIVAMPYINARLWDMRAKSYPEAIPSTAKDPTGTPYLEIYGPESGLLTP
                     MCTQTKLWQDTVFNLCRRIYEEIGANAIYLDQVAAMPAAPCYDPTHGHPLGGGHHWHD
                     GYRVILNRVKDYIASTGKDLFLTTENPGDAHIDGCDAFLIWNPRQDTEVPMMTSVYSG
                     YTLYYASNHTTGEGMQSFVMAQARDFLWGSQLGWMPPEIPEPFRGYLKKLGKLRVQTR
                     KFLTYGELVGMPRNTAPDEIRGSSDEMMRKREESIAKVSANWPFFGTPKIATLPAVMS
                     ALWRAEDGHLGLFIMNLSEVKQSYCCAFDPPAYGLTSERLRYTRIQENGPGDSFEVQG
                     GVQVRSEDLEPFGGVVYDIGPAGT"
     gene            5378..6559
                     /locus_tag="PLT86_09470"
     CDS             5378..6559
                     /locus_tag="PLT86_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HOT36881.1"
                     /translation="MPKGAVYPPEMSKYVDPVSGNEVVQLTSTRQWNVHPTYHVNGFL
                     DDQRIVFAAEAEGATNIYACSLVAGEITQLTAGRGTSYLLAYLAGERGDGRGTDAFHI
                     VAGYSSHAVYFVEGDELRTVNADTLEEESLYRIGPEWVCGVLELSGDEQTVLLPLLPR
                     ECFNTRCDLAEFLRRTDRLALPSRLVGVSVGDARSQVLWEDAGRFIGHAAFSPVSDKL
                     VLCERATDPDRRDEPLLWLLKLEDEEAIPFPTRNPKTGHSVWLPDGTGVVTHGMVIDG
                     GREHGAEYIQILNLDTSARWIGYHGPPRYYGHCHATPDGQTIIVDGVFTPDQITAVTP
                     HGDGYFTEPICVHGTDSSGGQRAHAHPHVSPDCEWLVFGSLRGGKKDLFAVRVKGLAK
                     G"
     gene            complement(6549..7778)
                     /locus_tag="PLT86_09475"
     CDS             complement(6549..7778)
                     /locus_tag="PLT86_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT36882.1"
                     /translation="MGDVEGLDRTSWWQNARFGMFIHFGLPTVLERGIWAQYWEHIPR
                     DEYAALKRSFNPESCDAVEWVRLARDSGMKYMVLTVRNHDGFCLFDTATTDFNSVASP
                     FGRDLTREFADACRAAGMRTGFYFSLMNWRHPGCLQRDQKQYRPPAFYERLVEETHEQ
                     VRELTSNYGKVDVLWFDCMRPDDPAIWRSEEMIAMVRRNQPSVLVNDRAGVPGDFGTP
                     ENEATPQSRPWEACFTMTDTWGHAPGDRNWKTSRELVALLMSCAARSGNLLLNLPPGA
                     DGRFPREGVDRLREIGKWLRTNGEAIYGTQSAPIGHALNVIGPGQGWATMRGSTIYMA
                     AMRWQGPTFRFAWLKNRVVSARVLGADCEVRVEQKGDRVWLHGLPEHPPDPLLPVLAI
                     TVEGTPERIEPGEFLIL"
     gene            complement(7771..>9062)
                     /locus_tag="PLT86_09480"
     CDS             complement(7771..>9062)
                     /locus_tag="PLT86_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOT36883.1"
                     /translation="FHLAAGALVKPSREKRDYPPVPNLPDSNYRAELLDNALNSRYAI
                     LYALNAVNVTVCGRGCIDGDDRAFWTKKNTGTYDEWNMIAQWFYYAPNAFRPVTLMFE
                     RCKNIVVRDVELVRASCYSGWFAGCTRLQFENVTVRNDHAGPNTDGFHFSSCRNVTIT
                     GCDFTCGDDCIAIDPNYGGIAENYTVSGCRFDTSVNAFRIYTGLDPGLPHEMPRGEVR
                     NVVASTCIVANASGVFNVTAENGEIHHLMFSDFCIQMAYRGSAFFFIALERGRINRVT
                     LSRMLIGTDGVGTIVSREKGAVRGIVLDGLDYVVSPRTKLYGNGAPPVLDSWGWHSFA
                     PYNLYVRAAGDVMIRDTKIVWTEGDLADLGKIPDGSPDWPAIECRDVNGLVLDNVICS
                     AHGENRPDVLLVNVKSPEVASCRRVDGERATVEERHG"
BASE COUNT         1956 a         2596 c         2650 g         1860 t
ORIGIN      
        1 ttcatcctcc gaatcgagga cacggaccag acgcgagtgg ttgagggcgc aattgaagcc
       61 attttcgccg gccttcgctg gctgggattg gagtgggacg aggggccgga tgtcggaggg
      121 ccggttgggc cctacgttca gtcgtgcagg cttgatatat accggcaaca cgtcgacaac
      181 cttatcgcga gcgggcatgc gtatcgttgt ttttgttcag aagagcgtct cgcggaatta
      241 cgtgaggccc agcgactcgc aaaaaagtcg cccggttatg acagaaggtg ccgcaacctt
      301 tcggcagaag agatccgaac tgccctcgat tcacacacac ccttcgtggt gcggatgaaa
      361 atcccgttga ccggcgagac acgttttgaa gacaggatcc gcggcacagt acagtacgac
      421 aacaaggtgc tggacgattt cgtcctgttc aagagtgatg ggtacccgac ataccacctc
      481 gcgaacgtag tggacgatca tctcatgggt attactcacg taattcgagg cgaagagtgg
      541 atccccagta cgcccaaaca cgtgctcctt tacgacgcgt tccggtgggc tgactcgcta
      601 ccggaattcg ttcacgtacc ctccattctg tcccctgacg gggggaagct ttcgaaacgg
      661 aagggcgctg cagcggtaac ggatttcgcg gagatgggtt acctgcctga agccgtccgg
      721 aacttcatcg cgttgctggg gtggtcgccg ggcggagacc gcgaaaaaat gacccttgat
      781 gagatgatcg aggcgtttgc gctggatcgc gtgaacccca aaggcgcaat cttcgacgaa
      841 tcgaagcttg agtggttgaa cggcgagtat atgaaagaca tggcggccgt ggacctgcta
      901 ccacgcgttg cgcctttgtg gatagagaaa ggtctgattg cagaaaacga ccttgaaaac
      961 gaccgcgagt acctctgcca tgtgatcgaa ctcttcaaag gccggacgcg ccgcacccac
     1021 gattttgtgg atctcgccgg tttctgcctt cgtgccccca cgtcgtacga tgagaaggcg
     1081 cgggcgaaac actggagtga tcctgcagca gccgatcgtc tggctgctgc cgcggacgca
     1141 ttcgcgaacc tggaaccctt cgacgctcgg accaccgaag aagtcgttcg gcttctgagc
     1201 gaaaaggagg gtgtgagcgc atccagatac atccacccga tacggctcgc cgtaagcggc
     1261 atgtcgttcg gcccgggcct ttttgagatg ctcgaagtgc tcggaaaagg acgcgtggtc
     1321 gaaaggttgc gaacggctgt gcacgttatc cgttccgacc gggtgggaac tgactgattc
     1381 gctgggcgtt attcctattg gaaacaggtg ttgccaattc gggctcgata caagcccgat
     1441 cggaactcac aaagaactga aagggggcga aaaggcttcg acgggagtag tggggctctg
     1501 gtcgcgcgcc gaggtgcact gcctcgctaa acaggtgcaa aactacgata actgacaact
     1561 acagttacgc aatggctgcc taagaggtag cccgtccccc tgcatactgc tcgcgcagtg
     1621 caggaagggg cgccgaacgc gcgagctggt tggtggggtg gcccgttccc gccaacgaga
     1681 ttcagggctg gcctgccgat atgctgagtc tgtccgggaa tcggcgggcg agacgtgatg
     1741 acggactacg cgcgtagtga tcggggtttg gctgctttcg gacgtgggtt cgactcccac
     1801 cgcctccacc atttcccctt tgcaactgcc ctggctgcgt ttgaccgcag gcggggcagt
     1861 tgcgcttttt cctcccccgt cacgttgcga ttcctttccg gttggaccta cattgacaca
     1921 tcgtgtaggc gaacagcagg atgtgccatc ggggaaaaca ttccagggga tcacaaatga
     1981 cggcaaaaat cgcctcgatt tgggaaatgc attatcaatg gattctcgac ttcgggtaca
     2041 aagtgttgct tacggtggtg gtgctggccg cgagctatct cgcagcaaag gccattcgga
     2101 ggtcaataac ccgggcgaac gaacgattcg aacgactgga cgcaaccctt gttcccgttc
     2161 tcactgcgct ggcgacctac gcagtgtatg cccttggaac aatcattgtc ctcgacattc
     2221 tgggcgtaaa cacggcgagc ataatcacgg tgctcggcgc caccgcgttg gcaatcggcc
     2281 tcgctctcaa ggacacgctc ggcaatctcg ccgccggcat catgttgctc attctgcgtc
     2341 catttcgagc cggggatttg atcaaatgcg gtactatcga aggcacggtc gaggaagcgc
     2401 atcttttcac cacaaagctt cagacaatcg acggcctgtt cgtggaagcc cccaattccg
     2461 agctgtggaa caaccatatc accaacttca cgcgcaacgg caaacgccgc atggacattg
     2521 tggtgggcat atcgtattcg gatcccatag acaagggtct ggaagtactg gcgcagctcg
     2581 tccgcgagga accgcggttc ctgaacgagc cgcctccaca gtccatggtg aaggctcttt
     2641 ctgacagttg tgtggaaatt cagttgcgag cttggacgag tattgacgac tactggaaca
     2701 cgtacttcga ccagaaccgg cgggtaaaac tggccatcga ggccgcaggg ctgaccatcc
     2761 cattcccgca acgagacgtt catgttgttt cggggaatat gccggggccg tcgcagtaga
     2821 attttcgaac agaggtagca ttctgtcgcc gacgtggcgg catttcgttc cttttcgtcc
     2881 gccggtgtgg agacaacacc cggcgggccg gctcgggagg gcacaatggt cctggataat
     2941 ggcgttctgc acctggaatt cgacgacgca accggtctcc tttcgcacct gatcgatgtt
     3001 cagggccgcg ttgttcatct ggcaccgccc gacgagccgc tgggaatctg gaagctcgtc
     3061 ttccgcgcgg gaaccgacga gcaatcactc gtgtggcctc cgtggaaagg tgaggtagag
     3121 atgagttgcg tccgagaatc cgacggaact caggtcgcat ccatcgaatg gcgtgacatg
     3181 tggttccgac acgagccgaa ggcggtggac gttgccgtga cagtacggct tccggaaaac
     3241 tcgggtactt ccgaatggcg gatacgcgtt tccaaccgga gcgctcggta cggcctttgg
     3301 gaggttcgct tcccctggtt caatcggtgg ccggctccga aggaatacaa cgtggcgatt
     3361 ccttggcaca actggggaca ccggtaccag aaggtcgcgg aaaggcacac gggcgaatac
     3421 tcgagctaca cctggtcgat gcagtttctc acgtttgaat cggggcaacg ggggctgaca
     3481 attgcggcct tggaccccga gcagcattac aaatcgtacc tggtggaccc ggggaacgaa
     3541 accgcgttcg ttcttcgcgt gccggacggt tccaagcctg gcgtcgggct ggacgacacc
     3601 tacgccacct cgatcagcgt gcacggcgac ggctggtttg gcggctgcaa gttgtaccgg
     3661 gaatgggcgc ttggacagaa gtggtgcgca aaagggccga tttcgaggcg aaaggatgtt
     3721 ccgaaaacgt tgaaagacat tggaatctgg cttctttcca gcattcccga gaagaacagc
     3781 ggagtcaccc ctgcggaatg ggcggacggg gttattgccg cgcaacgata tttcgacgta
     3841 cccgtaggga ttcagattta ccagtggcac gagatcaagt tcgacaacga ctacccggag
     3901 tatttccccc cgaaacccgg tgttccggaa gccgtgcgga aactcgttga tgccggaatc
     3961 gtcgccatgc cctacatcaa cgcacgattg tgggacatgc gcgccaagtc ttaccccgaa
     4021 gctatccctt ccaccgcgaa ggacccgacc gggactcctt acctcgagat ttacggcccg
     4081 gaaagtggtt tgcttacacc catgtgcaca cagacgaagc tctggcagga cactgtgttc
     4141 aatctctgca ggagaatcta cgaagagatc ggcgcaaacg cgatttatct cgaccaagtc
     4201 gccgcgatgc cagcagcacc ttgctatgac cccacgcacg gtcacccgtt gggcggcggc
     4261 caccactggc acgacggtta ccgcgtgatt ttgaacagag tgaaagacta cattgcctcc
     4321 acggggaaag accttttcct gacaacggag aacccgggcg acgcgcacat agatgggtgc
     4381 gacgcatttc tcatctggaa tccgcgtcag gacacggaag tgccgatgat gacctctgtc
     4441 tattccgggt atacgttata ctacgcaagc aaccacacca ccggcgaagg catgcagtca
     4501 ttcgtgatgg cgcaggcacg tgacttcctc tggggatcac agcttggatg gatgcctcct
     4561 gaaatcccgg agccattccg cggatacctg aaaaaacttg ggaaactgcg tgttcagacg
     4621 cgcaaattcc tgacgtacgg ggaactggtg ggaatgccac gaaacacagc cccggacgag
     4681 atacgcggca gctccgacga aatgatgcga aaacgagagg aatccattgc gaaggtcagt
     4741 gccaattggc cgttcttcgg aacgccgaaa attgccacgc tccctgcggt gatgtctgcg
     4801 ctatggcgcg cggaggacgg acacctgggt ctgttcatca tgaatctttc agaggtgaag
     4861 cagtcgtact gctgcgcgtt tgatccgccg gcgtatggtc tcacatcgga gcggttgcga
     4921 tacacgagga ttcaggaaaa cgggcctggg gattcgtttg aggtacaggg gggcgttcag
     4981 gtgcgatcgg aagacctcga gccattcgga ggcgttgtgt acgacatagg ccctgcgggc
     5041 acctgacaag cgggctccag gaaatgacat ggtgcaagga aaatccgtaa cggttccgcg
     5101 ccgcactgct ttcccggcga ggttgtcaca acctcgccgg gaggcatgca agcttcagag
     5161 aagtagcgat tgctcttgat cgcatccgag cggcgcacat gcccaccgac ggtgcaagtt
     5221 tgagggtgtt tgcggcgggc ataagacacc gacccgcacg cgacgaaatc tgctcaacgc
     5281 gaattgacat aattggtgtt atcggtctgt caaccgtgga caatgtggct cacgagaaga
     5341 tctgagcacg gctgcaatcg cctggagatg gaacgacgtg ccaaaggggg ctgtgtaccc
     5401 gcccgagatg tcgaagtacg ttgacccggt gtcgggcaat gaggttgttc aactcacgtc
     5461 gacgcgtcag tggaacgtac accccaccta ccacgtgaat gggttcctgg acgaccagcg
     5521 aattgtgttt gccgcggaag ccgaaggtgc gaccaacatc tacgcatgtt cacttgtggc
     5581 aggcgagatt acccagttga ccgcgggcag aggcacatct tatctcctgg cgtatctcgc
     5641 gggcgaacgc ggagacggcc ggggaacgga tgcatttcat atcgttgcag gttactcgag
     5701 ccatgccgtg tactttgtcg aaggagacga actgcgcacg gtgaacgctg acaccctgga
     5761 ggaggaatct ctttatcgaa tcggtccgga atgggtatgt ggagtactgg aactgagcgg
     5821 ggacgaacag accgtacttt tgccgcttct gccccgcgag tgtttcaaca cgcgctgcga
     5881 tctcgccgaa ttccttcgca gaactgatcg tctcgcgttg cccagccgcc tcgtaggtgt
     5941 gtcggtgggg gacgcgagat cgcaagtctt gtgggaagac gcgggacgct tcattgggca
     6001 cgcggcgttc tcaccggtct ccgacaagtt ggttctgtgt gagcgagcaa cggacccgga
     6061 tcgacgtgac gaaccattgc tgtggctgct gaaacttgag gacgaagagg caataccgtt
     6121 tccaaccagg aacccgaaga ccgggcacag cgtgtggctt cccgacggca caggtgttgt
     6181 tactcacggc atggtgatcg acggaggtcg cgagcacggg gccgagtaca ttcagattct
     6241 gaacctggat acgagcgccc gctggattgg ctaccacggc ccgccgcgtt actacggcca
     6301 ctgccatgct acaccggacg gccagaccat cattgtagat ggagtgttca cgccggacca
     6361 gataaccgcg gtcacgcccc acggcgacgg gtatttcacc gaaccaatct gcgttcacgg
     6421 aaccgattca tccggggggc aacgtgcgca cgcgcatccg catgtatcac cggactgtga
     6481 gtggctggtt ttcggttcgc ttcgcggtgg caagaaggat ttgtttgccg tccgcgtcaa
     6541 gggactggct aaaggatgag gaactcaccc ggttcaatcc gctccggcgt gccttccacc
     6601 gtgattgcga gcacggggag gagcggatca ggcggatgtt ccggcaaccc gtgcaaccag
     6661 acgcgatctc ctttctgttc cacgcgcacc tcgcaatcag cgcccagcac ccttgccgac
     6721 accacgcggt ttttcagcca tgcaaatctg aacgttggtc cctgccatcg cattgcggcc
     6781 atgtagattg tggaaccgcg catcgtcgcc catccctgcc ccggtccgat aacattcagc
     6841 gcgtggccga taggcgcact ttgcgtcccg tagatcgctt ctccattcgt tcgcaaccac
     6901 tttccgatct cccgcaatcg atccacgcct tctcgaggaa atcggccatc tgctccgggt
     6961 ggaaggttga gaagaaggtt ccccgaccgc gccgcgcagg acatgagaag cgcaaccagc
     7021 tctcgtgaag ttttccagtt ccggtcaccg ggagcgtgcc cccatgtatc ggtcattgtg
     7081 aaacacgctt cccagggcct ggattggggg gtagcctcat tctccggtgt tccaaagtcg
     7141 cccggtaccc cggcccgatc attcaccagg acggagggct gattccgacg gaccatcgct
     7201 atcatttctt ccgaccgcca aatcgcggga tcatccggtc tcatgcaatc gaaccagagg
     7261 acgtctacct tgccgtaatt actcgtcaat tcgcggacct gttcatgagt ttcctccacg
     7321 agacgttcgt aaaacgcggg tggccggtac tgcttctgat cccgctggag acagccagga
     7381 tggcgccagt tcataagcga aaagtagaag ccggtccgca ttcctgccgc gcggcacgcg
     7441 tcggcgaact cacgcgtcag gtctctaccg aaaggggatg cgacactgtt gaaatctgtc
     7501 gtcgccgtgt cgaaaagaca gaaaccatcg tggttgcgaa ccgtaagaac catgtatttc
     7561 atgcccgaat ctcgggcgag acgcacccat tctacagcgt cacacgactc cgggttgaac
     7621 gaccttttga gagccgcgta ctcatcccga ggaatgtgtt cccagtactg ggcccagatg
     7681 cctcgttcta agacggttgg caaaccgaaa tggatgaaca tcccgaagcg agcgttttgc
     7741 caccacgacg ttcgatccag cccctcgacg tcacccatgc ctttcctcca ccgttgcacg
     7801 ctcaccatcc acccgccgac aggatgcgac ttcagggctc ttcacattca caagcaggac
     7861 atcggggcga ttctccccat gggcggaaca gatgacgtta tccagcacga gaccgttcac
     7921 gtcacggcat tctatcgcgg gccagtcggg gctgccgtcc ggaattttgc ccagatccgc
     7981 gagatctcct tccgtccaga ctatcttcgt atccctgatc attacatcgc ctgccgcgcg
     8041 cacatacaag ttgtaaggcg caaaactgtg ccagccccag ctgtcgagca ctggaggtgc
     8101 tccgttgccg tacagcttcg ttctgggtga gacgacatag tccagtccgt cgagtacaat
     8161 tccccgcacc gcgccttttt ctcgtgagac gatcgtcccg acaccatccg tgccgatcag
     8221 catccgggag agcgtgacgc ggttgatgcg cccccgttcc aaggcgatga agaagaatgc
     8281 cgatccgcgg tacgccatct gaatgcagaa atccgagaac atgagatgat ggatctcccc
     8341 attctccgcc gtaacgttga aaacgcctga cgcgttggcg acaatgcacg tgctcgcgac
     8401 gacattccgt acctctcccc tgggcatttc atgtggcaac ccaggatcca gtccggtgta
     8461 gattcgaaac gcgttcacac tcgtatcgaa tcggcacccg gaaactgtgt agttctccgc
     8521 gataccacca taattcgggt cgatggcgat gcaatcgtcc ccgcaggtga agtcgcaccc
     8581 ggtgatcgtg acattgcggc acgaactgaa gtggaatccg tcggtatttg gacccgcgtg
     8641 gtcgttcctc acggtcacat tctcaaactg gagtcgcgtg caccccgcaa accagcccga
     8701 ataacaggat gccctgacca actccacatc gcgaaccaca atgttcttgc atcgctcaaa
     8761 catcagcgta acaggccgga atgcgttggg agcgtaatag aaccactgcg cgatcatgtt
     8821 ccattcgtcg tacgtgcctg tgtttttttt cgtccagaat gcgcggtcat caccgtcaat
     8881 acagcccctt ccgcagacag ttacattcac ggcgttcaga gcatacagaa tagcatagcg
     8941 cgagttgaga gcgttgtcga gcagttccgc ccggtaattc gaatccggaa ggttgggaac
     9001 tggcggataa tcccttttct cccgcgaggg cttgaccaag gctcccgctg ccaggtggaa
     9061 gg
//
LOCUS       DAOSOO010000125         9036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7665_length_9036_cov_5.686561, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000125 DAOSOO010000000
VERSION     DAOSOO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7665_length_9036_cov_5.686561"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLT86_09485"
     CDS             <1..311
                     /locus_tag="PLT86_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36884.1"
                     /translation="RMNVNTPEDLLVGNARLLEHNGTTIPLTTGCIVSPPVHIPASVQ
                     IGKGALIGPFVSLGEGCVIGKDVRIADAVVLSGAVIPDGAEIDRAIVDSNGDVYRVEG
                     "
     gene            complement(315..1307)
                     /locus_tag="PLT86_09490"
     CDS             complement(315..1307)
                     /locus_tag="PLT86_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT36885.1"
                     /translation="MSSTSLSETIGVAREDRAEKHEIRPRWTRYARTPLALAADLLFL
                     TRPVLLGPVWVVYGAGAVLVGSSPGIDLLFVSFVVAGVYIHNQLTDIPNDRANRKLFL
                     LSDGHVSHRAALTLTAMLWSAGILWSATQGIRSVLFALAIVLGLLYNGLRQPGWKSRP
                     LLGLVANALAHGVITFVAGFTAAGGTIFEGIVRSIPYAFAVAAVYLATTIVDENGDRL
                     TNKRTFCVVYGRAACTRWIAGLVCAAAVTAAANGDGWMAAAAIVSLVPAFLLLRAPAV
                     GNAQRAAVIAVSVLALAVAVRWTTLFVLAAATFAATRYYYRLRFGVVYPTLGSG"
     gene            complement(1324..2934)
                     /locus_tag="PLT86_09495"
     CDS             complement(1324..2934)
                     /locus_tag="PLT86_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOT36886.1"
                     /translation="MPNCRFNDQVLPIVVGKTLFEHADTNPAIRVPSSCNRVGTCHEC
                     IVQVHEGIEALTPRTDSEQFLSGNYRLACQAKVIRDDMEINVSTLKRKPKILTASEVL
                     DVPLDPLTRRSGDRVVRGNNDIDAFDGEVLGLAIDVGTTTVVAQLVDLTNGKRKLTAA
                     FENPQIFGGSNVLHRINYANNDPDHELQRVLIGHLNGELRRMGKRRHIYEAVVTGNPT
                     MRDLFFGLDVHSIGVKPFKSITEHEMLRGERTGTWLNASPRDLGLFMNGRGLVYGAPL
                     IACHVGADTAAGVLATRLFERERPSMLVDMGTNTEVVVGNKHRLIAASCPAGPAFEGS
                     GLRCGMPGLEGAVESFRLEDGNPVYSVIGDVTPRGICGSGVVDILAELTRNGIVDTVG
                     RFVDGRTEFVIDREQNIAVDRQDLSQLAQAKAANYAGQQILLRTFGIDWDDLEFLFFS
                     GGFANYLNVPNAQAIGLIPPIDPAKVMKVGNTALEGAAQMLINKGLRERIEQVVLTIE
                     HIELEREPDFFDMYVEGCLLEPMGRLKG"
     gene            3048..3947
                     /locus_tag="PLT86_09500"
     CDS             3048..3947
                     /locus_tag="PLT86_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT36887.1"
                     /translation="MKSKIGHAAESGHPRRFPLPVAATCVYVVLNIYRVLVSTVSIGH
                     TSMEQLLSPLRSINVVLVRPESPGNVGAVARVIRNFGLGELRIVGSDIDTDGPAARLA
                     HRSLGVLKDARFYEDVDSAVADAEWVIGTSGRLRSGRPMPATARSAASRIVAQCSHGA
                     GAILFGPESDGLSLRDLAHCDELIRVPQRRDAPALNLAQAVAVVGAELFQAALVPGTT
                     PVPPSVTRETRKRFVRRLKALSTHCGLSVRNRPEETLEELEGIFVRRRFEPHEMAVLE
                     LWLSQLEWFTGMDQGSNGRGTAE"
     gene            3953..8227
                     /locus_tag="PLT86_09505"
     CDS             3953..8227
                     /locus_tag="PLT86_09505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT36888.1"
                     /translation="MRRRSSFLLASLVFTGAAACFASQWHEISLPASYERLHCMAMLT
                     PESGWAGGQGGLILRCRGGQWRKVSAPTRSDVIAMAFDDEENGWVAGFDAATQKSLLF
                     RYQRNRWTPIPVPVGLIVTAIASDGRGTLFVFGQGGRAFRLRQRVWVETNTPGYRTML
                     SGVSLPDGSVWAVGEFGSAWRWNGSSWQGTQTPVRSHLHAVTATKDGKVWAVGDEGVI
                     LLHEGNAWRRIASPSSKSLYAVSARSVSEAWFGGERVLLHWNGSEVETAPVDVRGVVR
                     GLDVFESRTGWAVGLGTVLRLDPHGARRSGEQATRFGFYRQELLPNALGIFGAAFGDC
                     NGDGLDDLFLVSMRDANHLLVNRGAGSFGDETGVRGLMGTVVTIPVVKEMAQFGASWG
                     DYDNDGRLDLAVAGWYGSTYLYRQNSARRFDDVTDRLGLREGPFSANSVTFADVNRDG
                     LLDLFVCNEHGSNVLWLNNGRGGWTDGTRRWGLTSRGGSKDAVFGDLDNDGDVDLFVC
                     NGSGRNLVYRNDGTRFVDVSTTCAAAGERAEHNGATLADLDNDGDLDIVTTTSDGQNR
                     IWRNNGDWRFEDASGIVGFASGPFSYGSAAGDFDNDGFLDLLIAGADGVTFFRNAGDM
                     RFIPVAVEGLTDVKDARAVAVADVDADGDLDVFVASKADFTDGFSDFERRRSACFFNR
                     LDLKTSISVRCEGVSSNRMAVGARVRLYRDTGALSHAGELVGMREIMASNGCRSQQSA
                     AAHFGVDPASRYVVRVTFPSGREVEQRNVRAGQRLVVAEMTGPAAVARRVGQTAWRSI
                     WSNTARRHVFPLVVVIPLLVWAFRNASQRLLWGSLVTNMFTVALSVAYALLAIATVSS
                     SWWVGVLVPAVTVSLIAFLVVGLSYWSGPRAADKVAFRRRLAALEARADVTGVLSESL
                     DTAELAKHALEEFSDLFPVTLSVLVLCKHSSDTIEIVLPAEPPIPGLAPGLPLPEAIA
                     ETSSAGPIIWEKDFGVPPARSGLVPLCVVLSARGARVGVILSFVARRQLGEAMANEAA
                     MRSYAAFVGMSLFNAQLQQHARMRDEAYRVWLKGQGLGIPADRVPSASAAHRELTRRL
                     AVIKKNPPAELGTFESLTGDSVAIQEVRKRLQQVAGTDTSVLLLGPSGTGKELAARAI
                     HGQSKRKSGPFVPVNCGAIPEGLLESELFGHTKGAFTGAYAAKSGVFQRAHHGTIFLD
                     EIAEMNPSAQIRLLRVLQERSVTPVGGETAVPVDVRVIAATHQDLGVAVAEGRFREDL
                     FYRLNVFPIHLPTLAERMEDIPALVAVLLYKISLKTNLPLTGMAEDAVERILAHPWQG
                     NVRELENCLERALLLAGGEIIRSVHLQFDTAGPGTTLIARNSTEAPVGRKLADVEQEL
                     IEETLRECDFNVSEAARRLGISRDILRYRIKKLNISREKIRRDTAQ"
     gene            8257..8427
                     /locus_tag="PLT86_09510"
     CDS             8257..8427
                     /locus_tag="PLT86_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36889.1"
                     /translation="MNGQILDQSCCLWISRGVISYGTGFPKAARSVTAGVSSVEYLYE
                     ETIADLMTTVAH"
     gene            8684..>9036
                     /locus_tag="PLT86_09515"
     CDS             8684..>9036
                     /locus_tag="PLT86_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36890.1"
                     /translation="MPRGGADLIPGEQSGAIAFGSHLSLDRPGAGERAAVVDRFRGLV
                     YRIYRDFGRDLPWRRTCDPYEILVSEVMLQQTQVGRVAQKYPEFLRAFPGFHDLYEAP
                     LSDVLKVWQGMGYTR"
BASE COUNT         1787 a         2489 c         2883 g         1877 t
ORIGIN      
        1 ggcggatgaa tgtgaacacg cctgaagatc tgctcgttgg caatgcgcgc cttctggagc
       61 acaacggaac gacaatcccg ttgacaaccg gctgtatcgt ctcccctccc gtgcacatac
      121 ccgcgagcgt ccagatcggg aagggcgctt tgatcgggcc gttcgtgagt ctgggtgagg
      181 ggtgcgtcat cgggaaagac gtcaggatag cggatgccgt cgtgctcagt ggcgcggtca
      241 taccggacgg agcggagatc gacagagcaa ttgtagattc caacggcgat gtgtaccgcg
      301 tggaaggctg aaactcagcc gctgcccaat gtcgggtaca ccacaccgaa gcggaggcgg
      361 taatagtatc ttgtggcggc gaatgttgct gccgccagga cgaacagagt tgtccagcgc
      421 acggcaacgg ccagggcgag aacgctcacg gcaataacgg cagcgcgctg cgcgttcccg
      481 acagcaggtg cccgcaagag gaggaacgcg ggaacaagtg acacaatggc agccgcagcc
      541 atccacccat ccccgttcgc tgccgcggtc actgcggccg cacagaccaa tccagcgatc
      601 caccgcgtgc acgcagcccg gccgtagacg acgcaaaatg tgcgtttgtt cgtcaatctg
      661 tcgccgtttt cgtcgacgat agtagtcgca aggtataccg cagcaacggc gaaggcatag
      721 ggtattgaac gcacgatgcc ttcgaaaatg gttccccctg cggcagtgaa gccggcgacg
      781 aacgtaatca ctccgtgtgc gagagcgttc gccacgagcc ccagcaacgg acgcgatttc
      841 caccccggct gccgcaatcc gttgtacaaa agccccagta caatcgccaa agcgaacaga
      901 acagacctga tcccctgcgt cgccgaccac aggattccgg ccgaccatag catcgcagta
      961 agtgtcaaag cagccctgtg gctcacgtgc ccgtcggaga gaagaaacag ctttctgttt
     1021 gctctgtcgt ttggaatgtc ggtgagctgg ttatggatgt acaccccggc gactacgaag
     1081 gatacgaaga ggagatcaat cccgggggaa cttcccacca gaacggcccc ggcgccgtac
     1141 actacccaca caggccccag caggacaggt cgtgtgagaa aaaggaggtc tgccgcaagc
     1201 gccagcggag tccgcgcata ccgggtccaa cgcgggcgga tttcgtgctt ttctgcgcga
     1261 tcttcccgcg cgaccccaat cgtttcggac agactcgtgg agctcatttc gggtttttcg
     1321 atgtcagccc ttcaatcggc ccataggttc cagcagacaa ccctcgacat acatgtcgaa
     1381 aaagtcgggt tcacgttcca actcaatgtg ctcgatggtc aagacgacct gttcgatccg
     1441 ttcgcgcagg cccttgttga tcagcatctg agcggcaccc tccagtgccg tattgcccac
     1501 cttcatgacc tttgccggat cgatcggcgg gatgaggcca atagcctgag cgttgggaac
     1561 gttcagatag ttggcgaagc ccccagagaa aaacaggaac tcaagatcat cccaatcgat
     1621 gccgaacgtt ctgaggagta tctgctgccc ggcatagttg gctgctttcg cctgtgcaag
     1681 ctgactcagg tcctgtcgat ccactgcgat gttctgctct cggtcaataa caaactccgt
     1741 tcggccgtcg acgaatctgc cgacggtgtc aacgatgccg ttgcgtgtga gctctgcgag
     1801 aatgtccacg acgccggagc cgcagattcc acgaggggtg acgtcgccga taacggaata
     1861 gacgggattt ccatcctcca ggcggaacga ttccaccgcg ccttcgagcc ccggcattcc
     1921 gcaacggagc ccgctccctt cgaacgcggg tcctgccggg cacgaagcgg caatgagccg
     1981 atgtttgttt cctaccacca cttcggtatt ggtgcccatg tcgacgagca tggaagggcg
     2041 ctctcgttcg aacaggcggg tagcgaggac tcccgccgca gtgtcagccc cgacatggca
     2101 cgcgataagg ggagctccgt acacgaggcc ccggccgttc atgaagaggc caagatcgcg
     2161 tggcgacgcg ttcagccacg tgcccgtgcg ctcaccccga agcatctcat gctcggtgat
     2221 ggatttgaac ggtttcacgc ctatgctgtg gacatccagt ccgaagaata gatcccgcat
     2281 ggtggggttg cctgtcacca ccgcctcata gatatggcgc cgcttgccca tgcgtcgcag
     2341 ctcgccgttc aggtgcccta tcaaaacgcg ctggagttcg tggtcagggt cgttgttggc
     2401 gtagttgatc cggtggagta cgttacttcc tccgaaaatc tgggggtttt cgaatgccgc
     2461 ggtgagcttt cgtttcccgt tggtgaggtc gacaagttgg gcgacaacgg tcgtcgttcc
     2521 cacgtcgatg gcgagtccca gcacttcgcc atcgaatgca tcgatgtcgt tgttcccgcg
     2581 aacaacgcgg tcgcccgaac ggcgggtaag cgggtccagg ggaacgtcga gcacttcgct
     2641 tgcggtcagg attttgggct ttcgcttcag cgtgctgacg ttgatctcca tgtcatcacg
     2701 gatcaccttc gcctgacacg caagtcggta atttccggaa aggaactgct ccgaatcggt
     2761 tctcggggtg agcgcctcga ttccctcgtg cacctggact atgcactcat ggcacgtgcc
     2821 tacgcggttg caggagctgg ggactcgaat cgcggggttc gtgtctgcgt gttcgaaaag
     2881 tgttttgcca acaacaatgg gaagaacctg gtcattgaaa cggcagttgg gcactggtgg
     2941 actcctgagc gcgggacact caacagcacg gctgttgcgc agggtgtgtt tgatgcgggg
     3001 acatttccgt ttccgaggtg ccgacgcctg gcactgcggt gaatacgatg aaatcaaaga
     3061 tagggcacgc cgccgaatcc ggccaccccc gccgctttcc tttgcccgtt gcggcaacct
     3121 gtgtgtacgt tgtattgaac atttaccggg ttctcgtcag cacggtttcg attgggcata
     3181 cgagcatgga acagcttctc tccccgcttc gatctattaa cgtggtgctc gtgcgaccag
     3241 agtcgcccgg taacgtcggg gcagtcgcgc gagtgatacg caatttcggt ctcggagagt
     3301 tgcgtatcgt tggttctgac atagacacgg acgggccagc cgccagactt gcccaccggt
     3361 cgcttggcgt actgaaggac gcgcgtttct acgaggatgt ggactccgcg gttgctgacg
     3421 ccgagtgggt catcggcacc agcgggaggt tgcgttccgg aaggccaatg ccagcgacgg
     3481 ctcgctcggc agcctcgcgt atcgtggcgc aatgttcaca cggcgcgggt gcgatccttt
     3541 tcgggccgga atcggacggg ctttcattac gtgacctcgc ccattgcgat gaactcatac
     3601 gcgttcccca gcgcagggat gctccggcac tgaatctcgc gcaggcggtt gcggttgtcg
     3661 gtgcggagct ctttcaggcc gcactggtgc ccggtaccac ccctgtgccg ccttcagtga
     3721 cgagagagac gcgcaaacga tttgtgcggc ggctcaaagc gctgagtact cactgcggcc
     3781 tctccgtcag aaaccgcccg gaggagactc tggaagagct cgaagggata tttgtcaggc
     3841 ggcgctttga gccacacgaa atggcggttc tggagctatg gttgtcgcag cttgagtggt
     3901 tcaccggaat ggatcagggg tccaacggca gaggtacagc ggagtgacca caatgaggcg
     3961 cagatcctcc ttccttctgg catcgctcgt tttcaccggt gcggccgcgt gcttcgcatc
     4021 gcagtggcac gagatttccc tgcccgcgag ttacgagcgt ctgcattgca tggcgatgct
     4081 gacgccggag tctggctggg cgggcggaca gggcggtctg atacttcggt gtaggggagg
     4141 gcagtggagg aaagtctcgg cgccgacacg aagcgacgtc atcgcaatgg cgttcgacga
     4201 cgaagagaac gggtgggtgg ccggttttga tgccgcaact cagaagtctc ttttattccg
     4261 ttaccagaga aatcgctgga cgcccatccc ggttcccgtg gggctgatcg tgactgcgat
     4321 tgccagtgat ggccgtggaa cacttttcgt gttcgggcag ggcggacgcg cgtttcgact
     4381 ccggcaacgc gtgtgggtgg aaaccaacac gcccgggtac cgaaccatgc tgtccggcgt
     4441 cagcctcccg gacgggagtg tctgggctgt cggcgaattc ggttccgcat ggaggtggaa
     4501 cggatcaagc tggcagggga ctcagactcc tgtgcgctca cacctgcatg cggtaacggc
     4561 cacgaaggac gggaaggtct gggcggtcgg cgacgaaggc gtgattctgt tacatgaagg
     4621 aaatgcctgg cggcggatag cgtctccttc ctcaaaaagc ctctacgcag tgtccgcccg
     4681 gagtgtttcc gaggcgtggt tcggaggtga aagagttctg ctgcactgga acggttcaga
     4741 agtggagaca gcccccgttg atgtgcgcgg agtcgttcgc gggttagacg tatttgaatc
     4801 gagaacaggc tgggcggtgg gactcggcac cgttcttcga ctcgatccgc atggtgcgag
     4861 gaggtccgga gagcaggcaa ctcgttttgg cttctacaga caggaactgc tgccgaacgc
     4921 gctcggaatc tttggagccg cattcggcga ctgcaacggc gacggccttg acgatctgtt
     4981 cctcgtgtcg atgcgtgacg cgaaccattt gctcgtgaat cggggcgctg gcagttttgg
     5041 cgacgagacg ggcgttcgtg gtctcatggg cacagtcgtg acgattccgg tagtgaagga
     5101 aatggcgcag ttcggtgcat cctgggggga ttacgataac gatggccgcc tggaccttgc
     5161 agttgctggc tggtacggtt ccacgtatct ctaccggcag aattccgcgc gccgctttga
     5221 tgacgtgacc gaccggctgg ggctgcggga aggtcccttt tcggcgaaca gcgtcacatt
     5281 cgccgacgtt aaccgagacg gtctgcttga cctttttgtg tgtaacgagc acggaagcaa
     5341 cgttctctgg ctcaataacg gaaggggagg atggaccgac gggactcgcc gctggggatt
     5401 gacctcccgc ggggggagca aggatgccgt gttcggtgat ctggataacg atggggatgt
     5461 cgatcttttc gtctgcaacg ggagcggcag gaatctggtg taccgaaacg acggcacacg
     5521 gtttgtggac gtatcaacca cctgtgccgc tgccggcgaa agagcggagc acaacggagc
     5581 gacgctggcg gacctcgaca acgacggcga tctggatatc gtgacgacca cttcggacgg
     5641 tcagaaccgg atctggcgga ataatggcga ctggaggttt gaagacgctt caggcatcgt
     5701 gggatttgca tccggaccct tcagctacgg gagcgccgca ggtgatttcg acaacgacgg
     5761 gtttcttgat cttctcatag ccggggccga cggggtgacg ttttttcgaa acgcggggga
     5821 tatgcgtttc attcccgttg cagttgaggg tttgaccgat gtcaaggacg cgcgtgcggt
     5881 agcggtggcg gacgttgacg ccgatgggga cctcgacgtt ttcgtagcga gcaaagccga
     5941 ttttaccgat gggttttcgg acttcgagcg gagacggtct gcatgcttct tcaacaggct
     6001 tgacctgaag acctcgatca gcgtgaggtg cgaaggggtc tccagtaaca gaatggccgt
     6061 cggggcgcgc gtgaggcttt accgcgacac gggggccctg tcacatgccg gcgagttggt
     6121 gggcatgcgc gaaatcatgg cttccaacgg gtgtcggagt cagcagagcg cggcagctca
     6181 cttcggtgtg gaccctgcca gccggtacgt agtccgcgtc acattccctt ccggtaggga
     6241 ggtggaacag cgcaatgtgc gggctggaca acgcctggtc gtcgcggaaa tgacgggacc
     6301 tgcggcggta gctcgccgtg tcgggcagac agcatggaga agtatctgga gcaacaccgc
     6361 gcggaggcat gtgtttccgc tggtggtggt gataccgttg ctggtctggg cgttcagaaa
     6421 cgcaagtcag cgtttgttgt gggggtcgct ggttacgaac atgttcacgg tagccctttc
     6481 agtggcgtac gccctgcttg cgattgctac ggtttccagt tcgtggtggg tcggtgttct
     6541 cgttccggca gtcactgtgt cgctgatagc ttttctggtg gtcgggctgt cgtactggtc
     6601 agggccgcgc gcggcggaca aggtggcgtt tcgacgtcgt cttgcagcac tcgaagcgcg
     6661 tgcggatgtg acgggggtgc tgtcggaatc gctcgatact gctgaacttg ccaaacatgc
     6721 gcttgaagag ttctccgacc tcttcccggt aaccttgtcg gtgcttgtgc tgtgcaagca
     6781 cagttcggac accattgaga tcgtgctgcc ggcggagccg ccgattcccg gcctcgcccc
     6841 gggtctccct ttgcccgagg caatcgcgga gacgtcttct gctggcccga tcatctggga
     6901 gaaagacttc ggcgtaccgc ctgctcggtc cgggttggtt ccattgtgcg tggtactgag
     6961 cgctcgtggc gcccgagtcg gcgtaatact gagttttgtt gcacgccgac aactgggcga
     7021 agcgatggca aacgaagcag ccatgcgatc atacgccgct tttgtcggca tgtcgctttt
     7081 caatgcgcaa ttgcagcagc acgcaaggat gcgagatgaa gcgtaccgag tgtggctgaa
     7141 aggacaggga ctggggattc ccgcggacag ggtaccgtct gcttccgcag cgcaccgcga
     7201 gctgacccgg cgccttgccg tcatcaagaa gaacccaccg gccgaattgg gcacgtttga
     7261 gagtctcacc ggggattccg ttgcgattca ggaggtacga aaacgtcttc aacaggtcgc
     7321 gggcacggat accagtgttc ttctgctggg gccttccggc acgggaaagg aactggcggc
     7381 acgagcgatt cacggccaaa gcaagcggaa aagcgggcct ttcgtgcctg tgaactgtgg
     7441 tgcaataccg gaaggccttc tcgaaagcga actgttcggt cacaccaaag gagcattcac
     7501 cggcgcgtac gctgccaaat ccggggtgtt ccagcgggcg caccacggta ccattttcct
     7561 tgacgaaatc gcagaaatga acccatctgc tcaaatccgg ctgctaaggg ttctgcagga
     7621 gcgcagcgtt accccggttg gaggcgagac tgcagttcca gtggatgtgc gggtgattgc
     7681 cgcaacgcac caggatcttg gggttgccgt ggcggaagga cgtttcaggg aagacctttt
     7741 ctaccgactc aacgtgtttc cgattcatct ccctactctg gcggagagga tggaagacat
     7801 cccggccctt gtggcagtac ttctctacaa aatctcactg aaaacgaacc tgcccctgac
     7861 aggaatggcg gaggacgccg tcgaacgtat tctggctcat ccctggcagg ggaatgtgcg
     7921 tgaacttgaa aactgcctgg aaagggcgct actgcttgcg ggaggggaaa tcattcgatc
     7981 ggtgcatctg cagttcgaca cagcaggccc cgggacaacc ttgattgcga ggaattcgac
     8041 cgaagccccg gtcggaagga agcttgccga tgtggagcag gaactcatcg aagaaaccct
     8101 cagggagtgc gacttcaacg tgtccgaagc tgctcggagg ttggggatat ccagggacat
     8161 tctgcgttac cggatcaaga aactgaacat cagtcgggag aaaattcgcc gcgacacagc
     8221 gcaatagtat tgcacctgag gattccaatc aaagagatga acgggcaaat cctcgaccaa
     8281 tcgtgctgtc tctggatctc ccgtggcgtg atttcctacg gaactggatt tccgaaggca
     8341 gcccggagtg tgacggcggg cgtttcgagc gtcgaatact tgtacgaaga gactattgca
     8401 gacctcatga cgacggtggc tcattgattg cacgaaatga tgatccccac cccggggaaa
     8461 cgcaactgtg cgcgtacgtg gagggctctc tgccgaaaga cgtggcgctc ggtgtgttga
     8521 gtcatgcggt aaggtgccgg tattgtttcg tgaggctcgt tcgagtgttt gatgcgttgt
     8581 cagtcgcaga cgggctcgcg cgacactaca agcgttgcag acgcgaacac aatcaagagc
     8641 gtacatgcgc tctgattgac tttgaagagg tatgtgcagt cgtatgcccc gaggaggtgc
     8701 cgatctgata ccgggggaac agagcggcgc tatcgcattt ggcagccatc tttcgttgga
     8761 tcgaccgggt gctggagaga gggctgcggt tgtcgacagg tttcgtgggc tggtataccg
     8821 aatttaccgc gatttcggac gcgatttgcc ctggcggcgc acctgtgacc cgtatgagat
     8881 tctcgtctct gaagtaatgc tgcagcagac tcaggtgggc cgtgtggccc agaaataccc
     8941 cgagttcctc cgcgcattcc cgggcttcca cgatctctac gaagcgccac tcagcgatgt
     9001 gctcaaagta tggcagggga tgggatacac tcgcag
//
LOCUS       DAOSOO010000126         9025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7678_length_9025_cov_5.150613, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000126 DAOSOO010000000
VERSION     DAOSOO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7678_length_9025_cov_5.150613"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PLT86_09520"
     CDS             <1..1547
                     /locus_tag="PLT86_09520"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HOT36891.1"
                     /translation="NLNGRRRERRKTPADHAGRWVPALKGVCVDPTGFLCVAPSCVGV
                     GESFPLKVKVLGPVRPIACSGNWNDPKPALRGPFNLNVDRKIQYHDNCLPEWRGALEV
                     DGEGRLTGNRDLVFDGVHQGTFPGDSRPIRVFDGFSFAEPGIHFIRLVDEKSGVEGWS
                     NPVNVTPEPPRSRIYWGDPHWQTFFSDGIRCPEELYAFARDEGFLDFGAITDHMEGVT
                     DRQWDYFQAVTNDYNRPGRFVTLIGQEWTNHNPAAGAPGHRNIYYRQSAGPVLRSNAS
                     DCDTLEKLWKKLDSISRVDSIAIPHHSANVIMGVDWEQGWNPKYEKAVEIHSVWGNSE
                     RSREDGNPMAIQHLNGEIRGRHVVDALRKGFRLGFVGGGDIHDGRPGDALHQESYPPF
                     PTRFWPEGFTAVTVPVLTREAVFDAMRDHATYATTQSRIYLDVRAKPTRNGLTVAVTA
                     ASEEGVVEASIVANGCDVAALRSDDDPRVLRDRKTTVNVDPGGFCYVRIITDRGNMAW
                     SSPVWV"
     gene            1725..3944
                     /locus_tag="PLT86_09525"
     CDS             1725..3944
                     /locus_tag="PLT86_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36892.1"
                     /translation="MAARHQFLLFAALVFLFTPLRRATGVSGTAVSGEIHTTTWTKAG
                     SPYRVTNTIRVPQGQLLTIEPGVDVLFDDDVPFVVEGRIHAVGMQTDSIRFLPGTTEW
                     GGLRISGGDTSTLAYVRISGGNANGNPDSLWNRGGGLFVNVARVGVSHSVISGNSAAM
                     RGGGLYITDPASHVTLTNCCITDNATSPGGSNHGGGAYIYSSTVLLKHTLIAGNSAGG
                     EGGGLYVFGQSAVNVTQCTLVDNNAGEVRTGAIGCGNGAEITLNSSIVWNSGGIPAPD
                     TCHVTYTAIEAGYPGEGNTTADPLFVNAAAGDYRLGSGSPCINTADPALPADPDGTRA
                     DMGAYAYVNPSSELILPQVIAHAGTTVPFPITARVADAFGADMAFLLDAGLVESIRVI
                     STAWGETDTTSLAVSGDTVRVSISSAEKRTIVNDVLAELSITLRMDAAVCTVPVTWVP
                     ELTDVNEEPATLRDGSLEIRALLYGDVTGDGSLTGLDAAEMLKYRVRLRPTINLTLAD
                     VTGNGDVSHLDAALVLYKILHPEYVFPVLGGFVPRPSRGEPRELFWQRDGRSWALMVD
                     DGAGIRGGELAIRSSGLRTVTAPEGLVFANLTDGLLAIAFARGNVSGTLLLRIETADA
                     TAPEIVTASLDEGAIPVVWRGAGAALLLAPNSPNPFNPLTTITFSLPESGNTRLAIYS
                     ADGRLVRELMNGASEAGVHRVTWDGRDTEGRDAASGIYLCRLTCRSETLVRRMVLVR"
     gene            3934..4704
                     /locus_tag="PLT86_09530"
     CDS             3934..4704
                     /locus_tag="PLT86_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36893.1"
                     /translation="MFAETQAVRAGGSRLRAVVLATFILTGTAPAALVDPPRLVVPFS
                     VSGDSVTVVPVLITSSHSFGADLAFLIDTTVARPDRDFIRAHAFESEPSSLCLWNAVG
                     DTVFVSVSSPHPRDLSDVPLVELAFRGKRDVPSGSATALTLLPYPFSTFNEHGLSVAA
                     SDKSTTPHFSLSAAPNPFNSATAIRTSVPCTVVARLTVYATDGRAVRTLTPTAAAGMH
                     TFVWDGRDEAGRRVASGVYLCRLEASHQTSVIRVTVIR"
     gene            complement(4777..6414)
                     /gene="groL"
                     /locus_tag="PLT86_09535"
     CDS             complement(4777..6414)
                     /gene="groL"
                     /locus_tag="PLT86_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT36894.1"
                     /translation="MAAKLISYNVEARESILKGVNVLANAVRVTLGPKGRNVVIDKKW
                     GSPTVTKDGVTVAKEIELPDKFENMGAQMVKEVASKTSDAAGDGTTTATVLAQAIYTQ
                     GLKNVAAGANPMELKRGIAEAVKVLVAELKAMSVPVAGHDQIAQVAAISANNEMEIGT
                     LIADAMEKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPHFVTDPDAMEAVLEDA
                     LVLIHDKKISSINDLLPLLEKVGQAGKELLVIAEDVEGEALATLVVNKLRGVLKIAAV
                     KAPGFGDRRKAMLEDIAVLTGGKVISEEAGLKLENTRIEDLGRAKRIVVDKDNTTIVE
                     GAGDHAAIQGRVKQIRKQIEDTTSDYDREKLQERLAKLAGGVAVVHVGAATETALKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRAMKALDSLKLEGDLKVGVDIVRRAIEEPC
                     RWIVQNAGREGTVVVQNILANKDKNYGFNAQTEEYGDLVKQGVVDPTKVVRTALENAA
                     SVSALLLTTEAMVAEKPEPKKPAAPAAGGHHHPHGGF"
     gene            complement(6442..6732)
                     /locus_tag="PLT86_09540"
     CDS             complement(6442..6732)
                     /locus_tag="PLT86_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT36895.1"
                     /translation="MTIKPLGDRVLVKPVEETEQVRGGIIIPDTAKEKPQQGVVEAVG
                     PGKRGEDGKILPMNVKAGDKVLYGKYAGSEVKIADVKYLIMGESDILGIIEN"
     gene            7027..7959
                     /locus_tag="PLT86_09545"
     CDS             7027..7959
                     /locus_tag="PLT86_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT36896.1"
                     /translation="MRVVVTGGAGFIGSHLCDALIRENHSVVCLDNLITGNLSNVEHL
                     FGHDRFRFIKHDVTEYIHIPGEVDFVFHFASPASPVDFERIPIQILKVGALGSHKTLG
                     LARAKGAGFLLASTSETYGDPLVHPQPETYWGNVNCVGIRGVYDEAKRFAEAMTMAYH
                     RYHHINTHIVRIFNTYGPRMRPDDGRAIPAFISQALKNEPITVFGEGRQTRSFQYIDD
                     LIRGILLLMESDEHLPVNIGNPNEYTILQLAEAIIELTGSKSEIVHRPLPPDDPKVRQ
                     PDIRKARERLGWEPVVPLREGLTKTIEWFKTHGA"
     gene            7961..8893
                     /locus_tag="PLT86_09550"
     CDS             7961..8893
                     /locus_tag="PLT86_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOT36897.1"
                     /translation="MVKANRPASTAFAALLACLCFIGLSVAAEPPGGERIIKAGDVLQ
                     ISVIGYAEFSKTVVVSPEGRIRYPVVSDRNIIGWTFSELENQITQQLATMLRAAPYVF
                     VDYAQTYTIRINILGQVRKPGVTEVPNGIDLQGALWIAGGPESSADLGDIQVQRRGLD
                     GTEEISVDLERFLFEGRLQDIVQMKDGDLVIVRGAPDADKVKVFGEVNKSGSYVVPFG
                     ATVLDMIYLAGGPTTAGTLSDVRWIRREGGRVVEQKLDFASLLRAGRTDEIPRVGRGD
                     VIIVQKRIVTLAAFVTALSLTIQVLTLYFLVQKL"
BASE COUNT         1666 a         2716 c         2724 g         1919 t
ORIGIN      
        1 gaaacctcaa cggtcgcaga cgtgaacgcc gcaaaactcc ggcagaccat gcgggacggt
       61 gggtccctgc tctaaagggg gtgtgtgtgg atccgacagg ttttttgtgc gttgcgcctt
      121 cctgtgtcgg ggtcggcgaa tcatttcccc tcaaagtcaa ggttcttgga cctgttcgcc
      181 ccatcgcctg ctcaggcaac tggaacgatc cgaagcccgc gttgcgtggg ccgttcaatc
      241 ttaacgtcga tcgcaagatt cagtatcacg acaattgcct ccccgagtgg cggggtgcgc
      301 tggaagtgga cggggaaggc aggctgacgg ggaaccgtga tctcgtgttc gacggtgtcc
      361 accagggaac atttcccggc gattcgcggc ccatcagggt tttcgatggg ttttccttcg
      421 cggagccggg catccatttc atacggcttg tggacgaaaa atctggcgtc gagggctggt
      481 cgaaccctgt caacgtcacc ccggaaccgc cccgttcgcg catctactgg ggcgatcccc
      541 actggcagac gttcttcagc gatggcatcc gctgcccgga agagctgtac gcattcgccc
      601 gggacgaagg gttcctcgat ttcggcgcca tcacggacca catggagggg gttaccgacc
      661 gccagtggga ttacttccag gccgtaacga atgattacaa ccggccgggg cggtttgtca
      721 cgctgatagg ccaggagtgg acgaaccaca atcctgcggc aggagcgccg gggcacagga
      781 atatctacta ccgccagagt gccggaccgg tcctgcggag caacgcttca gattgtgaca
      841 cgctcgaaaa gctgtggaaa aagctggatt ccatttcgag ggttgattcc atcgcgattc
      901 cccaccacag cgcgaacgtt atcatgggtg tcgactggga acagggttgg aacccgaagt
      961 atgaaaaagc ggtcgagatt cactccgtgt ggggcaacag tgagcgttcc cgcgaggacg
     1021 gcaaccccat ggccatccag caccttaacg gcgaaataag gggccggcat gtggttgacg
     1081 cgttgcggaa aggatttcgc ctcggtttcg tgggcggggg cgatatccac gacggacgcc
     1141 ccggagatgc gttgcaccag gaaagttatc ctccgtttcc gacgcggttc tggccggaag
     1201 ggttcacagc cgtcaccgtg ccggttctga cacgggaagc ggttttcgac gcgatgcggg
     1261 atcacgccac gtacgcaacg acccagagca ggatatacct cgatgtgaga gcgaagccga
     1321 ccaggaacgg cctgaccgtt gcggtgactg cggcatcaga ggaaggcgtc gtggaagcct
     1381 ccatcgtcgc gaacgggtgc gatgttgccg cgctgcgatc ggacgacgac ccgcgagtgc
     1441 tgcgggaccg gaaaacgacc gtgaatgtcg atccgggtgg tttctgttat gtcaggatca
     1501 tcaccgatcg gggcaacatg gcgtggtcca gccccgtctg ggtgtgagta cgaacgctca
     1561 ggcaccgcgc gttgcccggt cctgcggttg ccctgctctg atacggacgg gtgggtgact
     1621 acattcttgc cattcactgt gcatcattct tttgcatgcg taaggatctc gtcgggctcg
     1681 cgtgcagcgc ctctgcacgc gtgaggagga tgaggaggag atgtatggcc gcgagacatc
     1741 aatttcttct ttttgccgcg ctggtattcc tgtttacgcc tttgcgtcgg gcaaccggtg
     1801 tttccggaac cgcggtttcc ggtgaaattc acaccaccac ctggacgaag gcgggaagcc
     1861 cgtaccgcgt cacgaacaca attcgcgtcc ctcagggcca gcttctcacc atagaaccgg
     1921 gcgtggacgt gctgttcgac gatgacgttc cgttcgttgt tgaagggcgc attcacgcgg
     1981 tggggatgca gacggacagc atccgtttcc tccccggcac gacggaatgg ggtggcttgc
     2041 gcatctccgg cggcgatacc agcaccctgg cgtatgtgcg tatcagcggg ggaaacgcga
     2101 atggcaaccc cgattcgctc tggaaccgcg gagggggact tttcgtaaac gtggccagag
     2161 tgggtgtttc gcactcggta atttccggca actctgcggc gatgcgcggt ggaggcctgt
     2221 acatcactga tcctgcaagt cacgtcacac ttaccaattg ctgcataaca gacaacgcaa
     2281 catctcccgg cggcagcaac cacggcggcg gcgcctacat ctacagcagc actgtgctgc
     2341 tgaagcacac gctgattgcg gggaacagcg ctggaggcga aggcggaggg ttgtacgtat
     2401 ttggccagtc cgcggtgaac gtcacgcaat gtactctggt ggacaacaac gcgggggaag
     2461 ttcgtaccgg ggccataggt tgcgggaatg gagcggagat cacgctgaac agcagcatcg
     2521 tgtggaattc gggcggcatt ccggcgccgg acacgtgtca cgtcacgtac actgcaatcg
     2581 aagcggggta cccgggtgaa ggcaatacta ccgccgatcc gcttttcgtc aacgccgctg
     2641 cgggtgatta ccgcctcggt tcagggtcgc cgtgcatcaa cacggccgat cccgccctcc
     2701 cagccgaccc cgacggaact cgcgcggata tgggtgccta tgcgtacgtg aatccgtcgt
     2761 cggaactcat tttaccacag gttatcgcac acgccggaac aaccgtgcct ttccccatca
     2821 cggcacgtgt cgcggatgcc tttggcgccg atatggcttt cctgctggat gcaggccttg
     2881 tggagtccat ccgcgtgatc agcaccgcgt ggggagaaac cgatacgaca tcactggcgg
     2941 tatccggtga caccgtgcgc gtgtccatct catctgccga aaagcgcaca attgtgaatg
     3001 acgtcctcgc ggaactcagc atcactctcc ggatggatgc cgccgtctgc acggtgccgg
     3061 ttacgtgggt gccggagctg acagacgtga acgaggaacc ggcaacgctg cgggacggtt
     3121 cgctggagat tcgtgctctc ctgtatggag atgtcaccgg tgacggcagt ctcaccggcc
     3181 tcgacgccgc ggagatgctg aagtaccgtg tgcggttgcg tccgacgatc aatctcacgc
     3241 tggcggacgt caccgggaac ggcgatgtgt cgcacctcga tgcggcgctt gtgctgtaca
     3301 agatcctcca tcctgagtat gtctttccgg tgctgggcgg gttcgttccc aggccatcgc
     3361 gcggcgagcc acgggagctt ttctggcagc gggacggccg ttcgtgggca ctcatggttg
     3421 acgacggtgc gggaattcgc ggcggcgaac tcgcaattcg ttccagcggc ttgcgaaccg
     3481 tcaccgcacc agaggggctc gtgttcgcta acctgaccga cgggttgctc gcaattgcgt
     3541 tcgcccgcgg aaacgtctcg ggaaccctgc tcctgcgtat cgaaaccgcg gatgcaaccg
     3601 cgccggagat cgtcaccgcg tcgcttgacg aaggcgcgat cccggttgtg tggcggggcg
     3661 caggggcggc cctgttactc gcgccgaatt ctccgaatcc gttcaacccg ttaaccacta
     3721 tcacgttttc gcttccggag tccgggaaca cccgtctcgc gatttactcc gcagacgggc
     3781 gcctcgtccg cgagctgatg aacggggctt ccgaagcggg tgtgcaccgc gttacgtggg
     3841 atgggcgcga tacggaggga cgtgacgcgg caagcgggat ctacctgtgc cgtcttacct
     3901 gccgtagcga aacgctcgtt cggagaatgg tccttgttcg ctgaaacgca ggccgttcgt
     3961 gccgggggat ctcgtctgcg cgccgttgtg ctggcgacat tcatcctgac aggaaccgcg
     4021 ccggcagctc tcgttgaccc gccccggctt gtcgtcccgt tctccgtctc tggcgattcc
     4081 gtgaccgtgg tacccgtgct gattacgtca tcacattctt tcggtgcgga cctcgccttc
     4141 ctgatcgaca cgacggtggc ccggccggac agggacttca tccgcgctca cgcgttcgag
     4201 tccgaaccct cttcgctctg tctctggaac gcggttggcg acacggtgtt cgtgtcggtt
     4261 tcgtctcctc atccccgcga tctatccgat gtgcctctgg tggaactcgc ttttcggggg
     4321 aagcgcgatg ttccctccgg gagcgccacg gctctcactc tcctgccgta ccccttcagc
     4381 acgttcaacg agcacggcct ttcggtagcg gcgtctgaca aaagcaccac gccccatttc
     4441 tcgttgtccg cggcaccaaa cccgttcaac tcggcaacag ccattcgcac ctcagttccg
     4501 tgcaccgtgg tggcgcgtct cacggtgtac gcgacagacg gccgcgcagt gcgcacgctg
     4561 acgccgaccg ctgcagcagg aatgcatacc ttcgtctggg acgggcgaga cgaggccggg
     4621 cgtcgggtcg caagcggcgt gtacctgtgc cggttggaag cgtcacatca gacctcggtg
     4681 atccgtgtga ccgtgattcg ttagaaccct cggtgggaaa aaagaacggg gcggcgaata
     4741 agcctcgccg ccccgtgtac gctgtggcct gattgctcag aacccgccgt ggggatgatg
     4801 atgtccgccc gcagcaggag ccgccggttt cttcggctcc ggtttttccg caaccatcgc
     4861 ctcggtggtg agaagcagcg ccgaaacact cgccgcattc tccagcgcgg tgcggacgac
     4921 tttcgtcggg tcgacaacgc cctgcttgac gaggtcgccg tattcttccg tctgcgcatt
     4981 gaacccgtag ttcttgtcct tgttcgcaag gatgttctgg accacgacgg taccttcgcg
     5041 gcccgcattc tgaacgatcc agcggcaggg ctcttcgatt gcgcggcgca cgatatctac
     5101 cccgactttc agatcgcctt caagcttcag cgaatcgagc gctttcatcg cccgcagaag
     5161 cgcaacgcct ccgcccggaa cgataccctc ttccaccgcg gcgcgtgtag cgtggagcgc
     5221 atcttccacg cgggctttct tttccttcag tgcagtttcg gttgccgcgc cgacatgcac
     5281 gacggccacg ccgcccgcca gcttcgcaag gcgctcctgc agcttctcgc ggtcgtagtc
     5341 actcgtcgtg tcttcaatct gcttgcggat ctgtttgaca cgaccttgaa tggccgcatg
     5401 gtcgccggcg ccttcaacga tggtggtgtt gtctttgtcg acaacaatgc gtttcgcgcg
     5461 gccaaggtcc tcgatccgcg tgttctccag cttgagcccg gcttcttcgg agatcacttt
     5521 gccaccggtc agcaccgcga tgtcctcaag catcgccttg cggcggtcgc cgaatcccgg
     5581 cgccttcacc gccgcgatct tcaaaacgcc acgcagcttg ttgacaacca gcgtcgccag
     5641 cgcttcgcct tccacgtcct cggcgatcac gagaagctct ttgcctgcct gcccgacttt
     5701 ctccagcaag gggagcaggt cgttgatgga ggagatcttc ttatcgtgga tcaggaccag
     5761 ggcatcctca aggaccgcct ccatggcgtc aggatcagtt acgaaatgcg ggctgatgta
     5821 tccgcggtca aactgcattc cttccaccac gtcgagggtc gtttcgatgc tcttcgcttc
     5881 ctccaccgtg atgacaccgt ctttcccgac cttctccatc gcgtcggcga tcagcgtgcc
     5941 gatctccatt tcattatttg cgctgattgc cgccacctgg gcaatctgat cgtggcccgc
     6001 caccggcacg ctcatagcct tcagctcggc gaccagaacc ttgaccgcct ccgcgatacc
     6061 gcgcttgagc tccatcgggt tggcgccggc tgcaacgttt ttgagcccct gggtgtagat
     6121 agcctgggca agcacggttg ccgtcgttgt accgtcaccg gcggcgtccg aagtctttga
     6181 ggcgacttcc ttcaccatct gcgcgcccat gttctcgaac ttgtccggaa gctcgatctc
     6241 cttcgctacc gtcaccccat ctttcgtaac ggtcggcgat ccccactttt tgtcaatgac
     6301 gacgttccgg cctttgggcc cgagagtcac cctgacggcg ttggcgagaa cgttcacgcc
     6361 cttcaggatg ctctcacggg cctccacgtt ataggaaatc agtttggctg ccatgcgttt
     6421 atgcctccat aattacgggt ttcagttctc aataatgcca aggatgtcgc tctcgcccat
     6481 gatcaggtat ttcacatcgg cgattttcac ctcactcccg gcgtatttgc cgtaaagaac
     6541 cttgtccccc gctttcacgt tcatcggaag gattttgccg tcttccccgc gtttccccgg
     6601 gccgacagct tccactacgc cctgctgggg cttctctttt gccgtgtcag ggataatgat
     6661 acccccgcgc acctgttccg tttcttcgac gggcttgaca agcaccctgt cgccaagtgg
     6721 tttgatagtc atggccgctt atcccctttt tcgtgataca ggaaccagct tcgcggtgtg
     6781 acccgcagac gacaacaccc accccggagg atggaacgaa tgtcctgatt gattagcact
     6841 catctctggt gagtgctaat acaagatagg ttccgcgacg gcactttgtc aagttagaaa
     6901 tcctgtcctg caaaaaccgg cacgtcaggc cgtcagcacg tactccgctt gaccatctcc
     6961 cggccgcgtg gtagcatttg tgtgtgagcc atgcagcagt tgccccgcca cggtgaggag
     7021 gagcgcatga gagttgttgt taccggggga gcgggtttca tcggatcgca cctctgtgat
     7081 gcgttgatcc gggaaaacca ctccgtggtc tgtctggata acctcattac gggtaatctc
     7141 tccaacgtcg aacacctgtt cgggcacgat cgcttccgct tcatcaagca cgacgtgacc
     7201 gaatacatcc atatccccgg cgaagtcgat ttcgtattcc attttgcgag ccccgccagc
     7261 ccggtggatt tcgaacgcat ccccatccag atcctcaagg tcggcgccct cgggtcgcac
     7321 aaaacgctgg ggctcgcgcg ggcaaaaggc gccgggttcc tcctggcttc aacgtcggag
     7381 acgtacggtg acccccttgt gcatccacaa ccagagacgt actggggaaa cgtaaactgc
     7441 gtgggaatcc gcggcgtgta cgacgaagca aagcgcttcg cggaagcgat gaccatggcg
     7501 taccaccggt atcaccacat caacactcat attgtgcgta tctttaatac ctacgggccg
     7561 aggatgcgac cggatgatgg aagggcgata cccgcgttca tttcacaggc gctgaagaac
     7621 gagccgatca ccgtgtttgg cgaaggcagg cagacacgga gctttcagta cattgatgac
     7681 ctgatccgtg gtattctcct gctgatggaa tccgacgagc acctgccggt gaatatcggg
     7741 aacccgaacg agtatacgat cttgcagctc gcggaggcga tcatcgaatt gaccggcagc
     7801 aaaagtgaaa tcgtgcaccg cccgcttccg ccggatgacc ccaaagtgcg ccagcccgac
     7861 atccggaagg cgcgcgaacg cctggggtgg gagccggttg ttcctctccg cgaggggttg
     7921 accaaaacca tcgaatggtt caaaacgcat ggggcatgac atggtgaagg cgaaccggcc
     7981 tgcttcaaca gcgtttgcgg cgcttctggc ctgcctttgt ttcatcgggt tgtcggttgc
     8041 tgcggaaccg cccggcggtg agcgcatcat caaggcgggc gatgtgctcc agatctccgt
     8101 gatcgggtac gcggaattca gcaagactgt ggtcgtgtcg cccgagggaa gaatccgcta
     8161 cccggttgtt tcggaccgaa acatcattgg ctggacgttt tctgaactcg aaaaccagat
     8221 cactcagcaa ctcgccacca tgcttcgcgc agcgccgtac gtattcgtgg actacgcgca
     8281 gacgtacacg atcaggataa acatccttgg ccaggtgcga aaacctggtg ttaccgaggt
     8341 cccgaacggc attgatctgc agggggccct gtggattgcg ggcggtcccg aatcgagcgc
     8401 ggatctgggc gatatccagg tgcagagacg gggcctggac ggtacagaag aaatctccgt
     8461 ggatctcgaa agattcctgt ttgagggccg gctgcaggat attgtgcaga tgaaggacgg
     8521 ggatctcgtc attgtccgcg gtgccccgga cgccgacaag gtgaaggtgt tcggcgaagt
     8581 gaacaagagc ggttcctacg tggttccctt cggggcgacg gttctcgaca tgatctacct
     8641 tgcaggtggc ccaacaacgg cgggcaccct gtcggacgtg cggtggatca ggcgagaagg
     8701 gggccgcgtg gtcgagcaga aactggactt tgcgtcgctg ctaagggccg gcaggacgga
     8761 tgagattccg cgcgtcgggc gtggcgacgt gattattgtg caaaaaagga tcgtgacact
     8821 cgccgcgttc gtcaccgcgt tgagcctgac gattcaggtc ctcacgctgt atttcctggt
     8881 tcagaagctt tgaagtggca tttcgcgggt tcgatcgttg tttttggcgg ctgtgttctc
     8941 gcgatcttgg cggtttgtgg cgaaagccgg gcggaaacag gcctgaccgg atcaacggcg
     9001 cttctttacg tcccggtggc ggatg
//
LOCUS       DAOSOO010000127         9015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7688_length_9015_cov_6.947656, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000127 DAOSOO010000000
VERSION     DAOSOO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7688_length_9015_cov_6.947656"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..677)
                     /locus_tag="PLT86_09555"
     CDS             complement(57..677)
                     /locus_tag="PLT86_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT36898.1"
                     /translation="MEIDVDKPVRRNTRLAGFDYTQAGAYLITVVTRRRVCVFGDVVD
                     GEMRVNPAGEMVDAVWRELPAHYPGVKIDWHVVMPNHFHGIVVLTDANERAVGAGPRA
                     RPQPNADENGRPQGVAPTMGLSDVLQRFKSLTTNEYIRRVASDGWPPFSRHLWQRSFH
                     DHIIRDDDDLARVREYIALNPAQWADDEENLANAREHERRMGGLMP"
     gene            complement(808..2451)
                     /locus_tag="PLT86_09560"
     CDS             complement(808..2451)
                     /locus_tag="PLT86_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT36899.1"
                     /translation="MRAVIAALLFLVVSHAVAQTDTRVALGVLDLDSGTIPEAELRVL
                     SDRLRVELFNTGQFRIIERERIDAVLREQGFQLSGCTATSCVVEIGELVGVRKMVAGS
                     VGKVGDVYSLTLRIIDVESGAIERTAVQDCRCSLQQLLTSTIALAAAELAGVAPTVTQ
                     TLERGEGYGVLYVNSIPPGAVIYLNGTLRRERTPATIDRLPAGEHIVRAVLDSLVGEQ
                     RVRVTRDAVTKTTLSLQPGTGSLFITSEPPEASVTLDGRPAGTTPLLLPQVTVGTRTV
                     VFSHADFFPDSQRVTVRFNERTPVSARLVPCGYLDIQVTPATARVYLDSRELGRDQMR
                     LRLPLAQYRLTIVAAGYDTVRRAVTIGHGQTVVVRETLTSVFGGVRITSNPEGASVSC
                     PAAGIRGFTPFESDRLLPGSYRVRVEKAGRLPGEVNVAVIKGRTVSIHVNLAEDPDIV
                     RMRNERIKVRARWTTLVVGLAAAGFGAKWGFDANHAYGDRETAYTNYLSATTTATATQ
                     WRTAYENADRRGDRAAKKRNVSLGAAGALLGVSVVLTVR"
     gene            2638..4731
                     /locus_tag="PLT86_09565"
     CDS             2638..4731
                     /locus_tag="PLT86_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOT36900.1"
                     /translation="MTFLNPMFLFALAAAGIPVAIHFLGKRRPRRVAFSAMVFLEEVR
                     RQQFRRLKFRNALLLLLRTATIVCVVLAFARPTIKTNAGAFGHEGASAIVVVLDDGPG
                     MGYESSYGRRIDWARRQLSDMLSVVRPGDWAVLIRTSRPDLVLPFTRATLENLKSVSD
                     WSGDGVAALQHAADILGRSDAANREVYVVSDLAGPPWQSLRPVQWPDRTAGYLLAPAD
                     EPATNLAVDSVAAGNQFVRAGRPFSVRAIVRNTGTVDVNDASADLWFDNRRMQRQVVS
                     VPAGGVAAVTFSAVVPAPGWLTGRIELSDDPLIADNTRHFAVFVPAETRLAIIGDESP
                     SRAFLSALFTAADSTNPYSVAVGTERTIPRDSVEQADVIIVNERVPTGGNVPGWISEA
                     THRASAQSTARDGAGILFLTGPGTDLTAANQSLLPLFTGDRFAGLSTLSAGTGGYYSL
                     DSRSVSDPVLTSLLSSSARASQPRFFTFARLTGGTAHRVVSFTTGDPWLVRGEKEPAR
                     GMVLTTGLSPEWGDLAYRGVVVPLMHHLVHSLARTSPVAGEYPAGTSVRRPLVTAGET
                     IMLTAPDGLQRRVVPAGDEIPSLDLGVLTPRGCWSISADGNVTDMIAVNLHRDESELQ
                     RLSAGAFAGAVGLTSLTRLGTRPADDIVSARIGTELAVPFFLAAMACIAAELLLMRGI
                     AMGKRDAEAAAPPSV"
     gene            4855..5595
                     /locus_tag="PLT86_09570"
     CDS             4855..5595
                     /locus_tag="PLT86_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT36901.1"
                     /translation="MKARGNLPRHIAVIMDGNGRWAKQNGFSRITGHRHGVESVRELV
                     RASAELGIEVLSLYVFSTENWNRPRHEVIGLMRLLRQTILRETDELNQNNVRLRASGR
                     IDDLPPAAAKALREAITTTGSNTGLILNLCINYSGKAELVDAIREIVRSGIPAEAITP
                     ETVAGYLYTADLPEPDLLIRTSGEMRLSNFLLWQTAYTELWFTPVLWPDFRRPHLYEA
                     ISAFQQRQRRFGKTQDQLFVEPREIGPE"
     gene            5592..6437
                     /locus_tag="PLT86_09575"
     CDS             5592..6437
                     /locus_tag="PLT86_09575"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT36902.1"
                     /translation="MSLDRRNLALRILVAIIGIPLILFLLLTETWGVVALAAVLSYLG
                     AIEWADMTGLRKTPVLYGLAVVGTIALVVVGYIQRANLWSATVAAITVLAFVGTLVSR
                     WKEHGVVRPLGATIAGVVYVGFFALMIPIRRLQDGDALLVSLLFMIWLCDTAAYFGGS
                     LLGRHPLAPSISPKKSFEGAIFGFFGSIAGGVVGWLLFRPTTFIMAEFAFLGAAIGIV
                     GQVGDLAESAVKREIGIKDSSRLLPGHGGVLDRFDSLLFALPAVWFWLQTRAMLWGLV
                     VTPSF"
     gene            6438..7613
                     /locus_tag="PLT86_09580"
     CDS             6438..7613
                     /locus_tag="PLT86_09580"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT36903.1"
                     /translation="MKRRLSILGSTGSIGRAALAIAARQPERFEVVALAAGNQWQLLV
                     EQIRDFRPALVSVSAESSAEEIRRALGDDCPSILSGSEGLEAVATFEPADIVVSSLTG
                     GVGARPTYAAVRAGKDVALANKEAIVVAGEIIAREAASRGTRLLPVDSEISAIWQCLQ
                     AGRKQDVRRILLTASGGPFRSMPAEAFATITPERALAHPTWVMGQKVTIDSATLMNKG
                     FEILEVRWLFDIPLDKIEVLIHHQSVIHSLVEYEDGSVIAQLGIPDMTLPIQYALTFP
                     ERSDTGRKPLSLADVGQLTFGRPDVARFPALALAREASRIGGTMPAVLSGADEVAVRA
                     FLSGRLPFAGIPRLVEDTMARHTAIPHPTLGDALSAESWAQETASSLLPGASKKGRS"
     gene            7610..8959
                     /gene="rseP"
                     /locus_tag="PLT86_09585"
     CDS             7610..8959
                     /gene="rseP"
                     /locus_tag="PLT86_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT36904.1"
                     /translation="MSIQGTIAFLAALSVLVFVHELGHFLVAKWAGVRVETFSLFFGR
                     PLFSVRRAKGKGWQFRSFFWKWGLPDDAFEGDETEYAIRWIPFGGYVKMSGQSDFGDA
                     EDSGAPWEFTSKPVGARAAVIFAGPLMNFILAVIVFTWLNMTYGVVGRIGITPAPCMW
                     VGSVQAGSRADSLGVPVGARWISLNDSPLTSWKNVAQLLEKSARPVIALQTPAGDTLR
                     IPMQKNLADLHEFGVEWENGAVIGGVVPIEPANEAGMREGDTVRAVNGQPVASWAEMS
                     RTIRRNAGRETVIEVKRDTITTVLTVTPSVDIGVDIPEGGVRLSFVPAVGTAIVQTWV
                     VTVKIVKFIERLVTRAISPSYIAGPLGIFQMTGIAAEQGMGTYLMLVGTLSANLGFIN
                     LLPLAVLDGGLLVFLAFEAITKRRPSAKQQGYIQRIGMALLITMMILVTIMDIQRIF"
BASE COUNT         1562 a         3035 c         2739 g         1679 t
ORIGIN      
        1 ctgtggtcgc ccgttttcgt ctgcgttggg ttgcgggcgg gcacgggggc ccgccactac
       61 ggcatcaatc cacccattcg ccgttcatgt tcccttgcgt tggcgaggtt ttcctcgtcg
      121 tcggcccact gcgccgggtt caacgcgatg tattcccgca cccgggcgag gtcgtcgtca
      181 tcccggatga tgtggtcgtg aaatgaacgt tgccagagat gccgggaaaa cggcggccat
      241 ccgtcagacg ccacgcggcg tatgtattcg ttggtggtga gagatttgaa cctttgaagg
      301 acatccgaca accccatcgt aggggcgacc ccctgtggtc gcccgttttc gtctgcgttg
      361 ggttgcgggc gggcacgggg gcccgcccct accgcacgtt cgttcgcatc cgtcaacaca
      421 acaatcccgt gaaaatgatt cggcatgacc acgtgccaat caatcttcac acccggataa
      481 tgcgccggca attcccgcca taccgcatcc accatctcgc ccgccggatt cacccgcatc
      541 tccccgtcca ccacatcgcc gaacacgcac acgcggcgcc gcgtgacgac cgtgatcaaa
      601 tacgccccgg cctgtgtgta atcgaacccg gccaaccggg tattccggcg gaccggtttg
      661 tcgacgtcaa tctccatcga aatcctcgcc ccacaccgcc aacgctgcct ttcccctgcc
      721 gtcggaaggg caacactcct ttgttcccct cgcccaccgg gagagggtgc tgcgcgtggc
      781 gcagcttcgg gggtgaggga ccatccctca ccgcaccgtc aacacgacgg atacgcccaa
      841 caacgcccct gccgctccaa gagagacgtt gcgcttcttc gccgcgcggt cgccccgccg
      901 gtccgcgttc tcgtacgctg ttcgccactg cgttgcggtg gcagtggtgg ttgcgctcag
      961 gtagttcgta taggctgttt cgcggtcacc gtacgcgtgg ttcgcgtcaa acccccattt
     1021 cgcgccaaag ccggcggctg cgaggccgac gacaagcgtt gtccatcgcg cccgcacttt
     1081 gatccgctcg ttccgcatgc gcacgatatc aggatcttcc gccagattca catggatgga
     1141 taccgttctc cctttgatca ccgcgacgtt cacctcaccc ggcaaccgcc ccgctttctc
     1201 cacacggacg cggtacgaac cgggcagtaa tcggtcgctt tcaaacggtg tgaagccgcg
     1261 aatccccgcc gcagggcacg aaaccgaagc gccctccgga ttcgaggtga ttcgaacccc
     1321 gccgaacacg ctcgtgagcg tctcccgcac caccacggtc tgcccgtggc cgatggtgac
     1381 cgcccggcgc acggtgtcgt agcccgcggc aacaatggtg aggcggtact gggcgagggg
     1441 cagccgaagg cgcatctggt cccgccccag ctcgcgcgag tcgaggtata cccgcgccgt
     1501 ggcgggggtg acctggatat ccagataacc gcacgggaca agccttgccg aaaccggggt
     1561 gcgctcgttg aaacgaacgg tgacgcgctg cgaatcgggg aagaaatcgg cgtgcgaaaa
     1621 gaccacggtg cgcgtgccga cggtcacctg cggaagcagc aatggcgttg ttcctgcagg
     1681 gcgcccgtcc agcgttacgg aggcttccgg cggctcgctt gtgatgaaca ggctgcctgt
     1741 gccgggctgg agcgaaagcg tggttttcgt aaccgcatcc cgcgtcaccc gcacccgctg
     1801 ttcgcccaca aggctgtcca gcactgcccg cacgatgtgt tcaccggcgg gcagacggtc
     1861 aatggtggcc ggcgtcctct cgcgtcgcag cgtgccgttg aggtagatca ccgcgccggg
     1921 cgggatggaa ttcacgtaca gaacgccgta tccctccccc cgctcgagtg tctgcgtgac
     1981 ggtcggcgcc acacccgcaa gctccgccgc ggcaagcgcg atggtggagg tgagaagctg
     2041 ctgaagcgag cagcggcagt cctgcacagc ggtgcgctcg attgcgccgg attcgacgtc
     2101 aatgatgcgg agcgtgagcg agtacacgtc gcccaccttg ccgacagaac ccgccaccat
     2161 ctttctcacg ccgaccagtt cgccgatttc caccacgcag gacgtggccg tgcacccgga
     2221 aagctggaaa ccctgctccc gcagcaccgc gtcaatgcgt tcacgttcga taatgcggaa
     2281 ctggccggta ttgaacagct ccacgcggag gcggtcggaa agcacacgga gttccgcttc
     2341 cggaatcgtg ccgctgtcca gatcgagaac gccgagggct acccgcgtgt cggtctgcgc
     2401 caccgcatgt gagacaacca gaaacaggag agcagcgatc accgcgcgca tgaactcctc
     2461 ctcgtcaagc gcctttgccg tatcgcgccg cacctcttgt gcatgtgaac ggtacggaaa
     2521 tgcagcggag cagtcaaacg caatcgactc cgtgcgcggc tggcttctca cacccgtcgg
     2581 tgtccccacc acgcctcaag tatcggcgtt gacgcacgca gttcgtacat tcccaacatg
     2641 acgtttctca acccgatgtt cctgttcgcc ctcgctgccg ccggcatccc ggtggcaatc
     2701 catttcctcg ggaaacgccg gccccgccgc gtggcgttca gcgccatggt gttcctcgag
     2761 gaagtgcgcc ggcagcagtt ccggcggctc aagttccgca acgcgctgct ccttctgctc
     2821 cgcaccgcca ccatcgtgtg tgtcgtgctg gcgttcgcgc gcccgacgat caaaactaac
     2881 gccggcgcgt ttggccacga aggcgcaagc gccattgtgg tggtgctgga tgacggcccc
     2941 ggcatggggt acgaaagttc atacggcagg aggattgact gggcacgccg ccagctcagc
     3001 gatatgctct ccgtcgtgcg ccccggcgac tgggcagtgc ttatccgcac ctcccggccc
     3061 gacctcgttc ttcctttcac ccgcgcgacg ctggagaacc tgaaatccgt gtccgattgg
     3121 tccggtgacg gcgtggctgc cctccagcac gccgcggata tcctcggacg aagcgacgcg
     3181 gccaatcgcg aggtgtacgt cgtcagcgac ctcgccggcc caccgtggca gtcgctccgc
     3241 cctgtgcagt ggcctgaccg caccgcaggg tacctgctcg cgccggcgga tgaacctgct
     3301 acgaacctcg cggtggattc ggtggcggcg ggaaaccagt tcgtccgcgc gggccggccc
     3361 ttctccgtgc gcgccattgt caggaatacc ggaacggtag acgtcaacga tgcgagcgcc
     3421 gatctctggt tcgataaccg ccgcatgcag cggcaggtcg tctcggttcc cgccgggggt
     3481 gttgccgccg tcacattctc cgccgtcgtg ccggcgccgg gatggctgac cggcagaatc
     3541 gagctttccg acgacccgct catcgccgac aacacccgcc atttcgcggt attcgtgccg
     3601 gcggaaaccc gcctcgccat catcggtgat gaatcgccct cacgcgcatt cctctccgcc
     3661 cttttcaccg ccgccgattc gacaaacccg tactccgtag ccgtcggcac cgagcgcaca
     3721 attccccgcg attccgtcga gcaggcggat gtaatcatcg tgaacgaacg cgtgcccacc
     3781 ggcgggaatg tgcccggatg gatttccgaa gccacgcacc gcgcgtcggc gcagtcaacc
     3841 gcccgcgacg gcgccggtat cctcttcctc acggggcccg gaaccgacct caccgccgcg
     3901 aatcagtcat tgctgcccct gttcacgggc gaccggttcg cagggctttc caccctttcc
     3961 gcgggcaccg gcgggtacta ctcgctggat tcccgcagcg tatccgaccc cgtcctgacc
     4021 agcctgctga gctcctccgc cagagcgagt cagccgcggt ttttcacctt cgcccgtctg
     4081 accggcggca cggcccaccg cgtggtatcg ttcaccaccg gcgacccgtg gctggtgcgc
     4141 ggcgaaaagg agcccgcccg gggcatggtt ctcaccaccg gcctttctcc ggagtgggga
     4201 gatctggcgt accgcggcgt ggtggttccc ctgatgcacc acctcgtgca ctcgctggcg
     4261 cgcacgtccc cggttgcggg ggaatacccg gcgggaacct ccgtgaggcg tccgcttgtc
     4321 accgccgggg aaacaataat gctcacggct ccggatggcc tccagagacg ggtggtgcct
     4381 gccggcgacg agattccctc cctcgatctc ggcgttctga ctccccgcgg gtgttggagc
     4441 atctccgctg acgggaacgt aaccgacatg atcgcggtca acctgcaccg cgatgaatcc
     4501 gaactccagc ggctttccgc gggagcgttc gccggcgctg tcggactcac atcgctgacc
     4561 agactgggta ccagaccggc ggacgacatc gtctccgcgc gcatcggaac ggagctggcg
     4621 gtgccgtttt tccttgccgc catggcatgc atcgcggcgg agctcctgct catgcgcggc
     4681 atcgcaatgg gaaaacgtga cgccgaagcc gcagcgcctc catcagtttg aatgcgcccg
     4741 ccaaaaactg atattatgta gttaaccctg ttctgcaggt tggatctcag ttgcgtgtcc
     4801 cgcctttatg aggagcgcga tctcacgttg acagacgccg aacagcaggc cgccctgaaa
     4861 gcgcgcggaa accttccccg gcacattgcc gtcatcatgg acggcaacgg gcgatgggcg
     4921 aaacagaatg gcttctcccg gatcaccggc caccgccatg gagtggaatc cgtgcgcgag
     4981 ctggtgcgcg cctccgcgga gcttggcatc gaagtgctgt cgctctacgt gttctccaca
     5041 gaaaactgga accgcccccg gcacgaagtc atcgggctga tgcgccttct caggcagacc
     5101 atcctgcggg aaacggacga gctgaatcag aacaacgtcc gcctgcgtgc ctccggccgg
     5161 attgatgacc tcccgcccgc cgcggcgaag gccctgcggg aggcaatcac gacaaccgga
     5221 agcaacacgg gactcattct gaacctgtgc atcaactact ccggcaaggc tgagctcgtg
     5281 gacgccataa gggagatcgt ccgaagcggg attcctgccg aggcgatcac tccggaaacg
     5341 gtcgccgggt acctctacac ggctgatctg ccggagccgg acctgttgat tcgcaccagc
     5401 ggcgaaatgc gcctcagcaa cttcctcctc tggcagaccg cgtataccga gctctggttc
     5461 acgccggtgc tctggccgga tttccgccgg cctcatttgt acgaagccat cagcgcgttc
     5521 cagcaacggc agcggcggtt tggtaaaaca caggaccaac ttttcgtgga accccgggag
     5581 atcggccccg aatgagcctt gacagaagga atctcgcgct gcgcatcctc gtggcaatca
     5641 tcggtattcc gctcattctc tttctgcttc ttacggaaac ctggggcgtg gtggcgctcg
     5701 ccgcggtcct cagttacctc ggcgcgatcg aatgggccga catgaccggg ctgcggaaga
     5761 cccctgtgct gtacgggctc gcggttgtcg gcacaatcgc cctcgtggtg gtggggtata
     5821 tccagagggc gaacctctgg agcgcaaccg tcgccgccat cacggttctg gcgttcgtgg
     5881 gaacgcttgt gagccgctgg aaggaacacg gtgtagtccg tccgctcggc gccacaatcg
     5941 caggggtggt gtacgtcggt ttctttgccc tgatgatacc gatccgccgt ctgcaggatg
     6001 gcgacgcact tctcgtctcc ctgctcttca tgatctggct gtgcgatacg gcggcatact
     6061 tcggcgggtc gttgctgggc cggcaccctc tcgcgccctc aatcagcccg aagaagtctt
     6121 ttgagggagc catattcggc ttcttcggct ccatcgccgg cggagtcgtg ggttggctgc
     6181 tgttccggcc gaccaccttc atcatggcgg agttcgcgtt cctgggcgcc gcaatcggaa
     6241 tcgtcggaca ggtgggagac ctcgcggagt cagccgtcaa gcgggaaatt ggcatcaagg
     6301 attcctcacg cctcctgccc ggccacggag gcgttctcga caggttcgac agcctcctgt
     6361 tcgcgttgcc cgccgtctgg ttctggctgc agacccgggc catgctctgg ggcctcgtag
     6421 tcaccccttc tttctgaatg aaaagacgcc tctccatact cggttccacg gggtccattg
     6481 gccgggcggc gctggcaatc gcggcgcggc aaccggagcg ttttgaggta gtcgcgctgg
     6541 ccgccgggaa tcagtggcaa ctgctcgtgg agcaaatccg cgatttccgg ccggcactgg
     6601 tctccgtatc cgcggaatcg tcagcggaag agattcgccg cgcactgggg gacgactgcc
     6661 cctcaatact ctccggcagc gaaggccttg aagcggttgc cacgttcgaa cccgccgaca
     6721 tcgtcgtcag cagcctcacc ggcggcgttg gcgcgcgccc cacctatgcc gccgtccgcg
     6781 cgggaaagga cgtcgccctg gccaacaaag aggccatcgt ggtggccggg gaaatcatcg
     6841 cccgggaagc cgcctcccgc ggcacccgcc tccttcccgt tgacagtgag ataagtgcca
     6901 tctggcagtg ccttcaggcg ggccggaaac aggatgtccg gcgcatcctc ctcactgcgt
     6961 ccggcggccc cttccggtca atgccggcgg aggcattcgc caccattact ccggaacgtg
     7021 cgcttgcgca ccccacctgg gtaatggggc agaaagtgac catcgactcg gcaacgctga
     7081 tgaacaaggg cttcgagatc ctcgaagttc gctggctgtt cgacatcccg ctggacaaaa
     7141 tcgaggtcct gatccatcac cagtccgtca tccattcgct ggtggagtac gaggacgggt
     7201 cggttatcgc gcaactcggc attcccgata tgacgctgcc gattcagtac gcgcttacct
     7261 tcccggagcg ctcggatacg ggaaggaaac cactcagcct tgcggacgtc gggcagttga
     7321 cgtttggcag acccgacgtc gcccgcttcc ctgcgctcgc actcgcccgc gaagcgtcgc
     7381 ggatcggcgg cacgatgccg gcggtactca gcggggccga tgaggtggcc gtgcgcgcct
     7441 tcctttccgg acgcttaccc ttcgccggca tcccgcgcct ggtcgaagac accatggcac
     7501 gccacaccgc cattccgcac cccacgctgg gcgatgccct ttccgcggaa tcgtgggcac
     7561 aggaaaccgc gtcttccctg cttcccggtg cctcaaagaa aggccgctca tgagcattca
     7621 gggcactatc gccttcctcg ccgcgctcag cgtgctcgtg tttgtccacg aactgggtca
     7681 cttcctcgtg gccaaatggg cgggcgttcg cgtcgaaact ttctccctgt tcttcggcag
     7741 accgctcttt tcggttcgca gggcgaaagg aaaaggctgg cagttccgct ctttcttctg
     7801 gaaatgggga ttaccggacg acgcgttcga aggtgacgaa acggaatacg cgatccgctg
     7861 gattcccttc ggcggctacg tgaagatgag cggacagagc gatttcggcg acgccgagga
     7921 ttccggggcg ccctgggagt tcacctccaa acccgtggga gcccgtgccg cggtgatctt
     7981 cgccggcccc ctcatgaact tcatcctcgc cgtcatcgtg ttcacctggc tcaacatgac
     8041 ctacggcgtc gtcgggcgca tcggcataac gcctgccccc tgcatgtggg ttggctccgt
     8101 tcaagccggt tcacgcgccg acagcctcgg cgttcccgtc ggagcgcgct ggatctcgct
     8161 gaacgattcg ccgctcacga gctggaaaaa cgtggcgcaa ctcctggaaa agagcgcgcg
     8221 tcccgtaatc gcgcttcaaa cgcccgccgg cgatacgctc cgcatcccca tgcagaagaa
     8281 cctggccgac ctccacgagt tcggtgttga atgggaaaac ggtgcggtaa tcggcggcgt
     8341 cgtgccgatc gaaccggcaa acgaggccgg catgcgcgaa ggcgacaccg tccgcgcggt
     8401 gaatggccag ccggtggcat cgtgggccga aatgagccgg accatccgcc gcaacgcggg
     8461 aagagagacc gtcatcgagg tgaagcggga taccatcacc acggtgctta ccgtcactcc
     8521 gtccgttgat atcggcgtgg acattcccga aggaggcgtc aggctctcgt ttgtgcccgc
     8581 ggtcggaacc gccatcgttc agacgtgggt agtcaccgtc aaaatcgtca aattcatcga
     8641 acggctggtg acgcgggcca tttccccgag ctacattgcc gggccgctgg gcattttcca
     8701 gatgaccggc atcgcggcgg aacagggaat gggcacctac ctgatgctgg tgggaaccct
     8761 cagcgccaac ctcgggttca tcaacctgct cccccttgcc gtgctggatg gcggcctcct
     8821 cgtgtttctt gcgttcgagg caatcacgaa gcggcggcca agcgcgaaac agcagggcta
     8881 catccagcgc atcggcatgg cgctgctgat caccatgatg atcctcgtca ccatcatgga
     8941 catccagcgc atcttctgag aagtcggcct gtagccgact tcttttgggg taggcctgcc
     9001 ttgtgggaaa ggcag
//
LOCUS       DAOSOO010000128         8983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7728_length_8983_cov_5.250896, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000128 DAOSOO010000000
VERSION     DAOSOO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7728_length_8983_cov_5.250896"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1005
                     /locus_tag="PLT86_09590"
     CDS             373..1005
                     /locus_tag="PLT86_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT36905.1"
                     /translation="MNDTEMSGVGDGTRAPADTGEKDRKTEIREKARSVFLRYGYRKA
                     TLDDIGKACGLSKTAIYHYFPDKEAVFAEVVRAESGVLLSKMRAAAASETDPKARLAE
                     VIRTRFRAMSEILNEVVGDARHEVREFLPKTAHAREQFFHEEGKLIEEILEDGGRQGV
                     FRVLRTRNGPLLLIAALQGVENGLAGLSDSLSIEEAIDEMLEILFDGLCR"
     gene            1097..2173
                     /locus_tag="PLT86_09595"
     CDS             1097..2173
                     /locus_tag="PLT86_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT36906.1"
                     /translation="MVWTTPLKRILLGIVASLPALCLSPLFAACGNKVQKTTSVEPGV
                     FVKPVIRVGNGRELHLSGTLAAERSIALSFATLGTVEQVMVEEGQEVKKGDVLAKLVS
                     RSYEDALAMAEANLNQAEDAYRRLEPMHRNGTLADVKMVEVETARQQARLAASIARKN
                     LDDTSLRSPVNGVIAHRNVEPGASASPVLPAFTLVQAKTLLATAPVPETQVSKIRRGM
                     SARVVIPALQKTVEGSVREISVVANPLTRTYDLKVAVANPTGELRLGMIADVYIKVDG
                     EVENLVIPPEAIRVDEVGNPYVYVVAADRRLQRRPVRVGGFVGEGTMVTAGVRAGESL
                     VVSGTPMLADGMTVRVLAPHANGE"
     gene            2177..6610
                     /locus_tag="PLT86_09600"
     CDS             2177..6610
                     /locus_tag="PLT86_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT36907.1"
                     /translation="MDPTSLTERAMRQWRITVTVVAVCVVAGVYSFFTMQRQEFPAFT
                     IRQGLVIGVMPGATSEEVEDQLARPVEDFLFSFNEVNKAKTYSVSKDGQLVVFVEVKE
                     DLDGIQTAAFWAKLRHGLNELKAQKLPGGVLALVGNNEFGDTSALLFTLVAEGKSPRD
                     LREYMKVLESHLRRIEATAKLRVIGEQNEVISINISRDRVARYGIRPATLWASLQGLG
                     ALPAYARLDGDELERPIYVRKVLRSENELAETILLSLPTGQHVRLKDVAEIKREYGHD
                     DAYVRFNGKTALVLSIEMQPGNDITRFGDEVDRALEETLHELPPGVTISRVVDQPKVV
                     RTSVNHFLRDFGLAIASVILVTMLLLPVRVAAVAAITIPVSIAITLAILNALGVELQT
                     VSLAGLIVVLGMIVDNAIVVIDDHVDKLDRGMDAWSAAWMSAHDLAVPVFTATIAIIL
                     SYVPMPRFLTGVAAEFLGSLPVTIAVALSVSLLIALLLLPIMNSQFVRRGLRKRSGQR
                     SMLERIQCVFDRALDAVFRHPWLTVAGGIGSVLIAVVIALRIPQQMFPKVDRDQFAVE
                     VYLPTGRSLKQTDAVMRRLEAELLADKRVVNVTSFVGQSAPRFHTLYAPYIPRRNFGQ
                     LLVNTIDADATLDVLRESEERLRGAFPECWVRWKQLEFQVGFPVEVRLSGNDAEALKT
                     TGAKIESYARGIAEVTWVHNDWEEALQSVDVMPDADACARLGVPPAMLQASLALGTQA
                     YRVGTIWEGDYPVRVLVRDDSGERGSVEGLRQQYVSSILGATVPLEQIASLRPAWHEG
                     AMVRRNGVRTLTVFIDIKKDALASEMQEKIEKYVSGLDLPGIRVSYGGEKELSTQIFS
                     PMTISMLVSIGLIFLVVLCQFQRFLTTLVVMLSMPLTLMGAFLGLVIMGYPFGFTAFV
                     GIIGLMGIAVRNGIILVTYAEELRVKQGLSAMEAARAAGKRRMRPIYLTSTAAAVGVV
                     PMILSRSTLWGPLGTVTFFGLLTAMVLTLFVLPVTYALVVGREDRRHEPSPKAPVVTG
                     LLLGLALAVSPASADETPLTLQECKELALQNNAQIRESNLEIEAARETRRAAYTKYFP
                     QISVSATGAVAVDPLARIHTSGGNLPVYNGNPATIPTATQFAYFPSGEMAIAGRGFVA
                     SAAVIQPLFAGGRIVSGNRLADLGVHVAKDRAEISKRDVLAQTEEKYWRLVALREKLR
                     TLDAYEALLEALDRQVTDAVQAGLLTRNDQLKVRLKRSEASVDRQRLENGVALATRDL
                     RRHIGLPEGGALRLVDTLACPDDPSILAQERAGALDRRPEIRLLSRAIRAEQLQNSLT
                     RGGMLPTVSVGAIGLRQDIRGMISQTNGLLMGMVNVPISGLWEGRHTTASQHRRVEIA
                     ENRLAETRELLDLQIENAWTDLTAAWRSNEVANEAVDQADVNLAEVSDRYRNGLVTFS
                     DLLEAQVLRQKATDSRIDARADYWLKRAAYLRAVAAADGSE"
     gene            complement(6675..>8983)
                     /locus_tag="PLT86_09605"
     CDS             complement(6675..>8983)
                     /locus_tag="PLT86_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36908.1"
                     /translation="TITPPLRPYFRDQTFDMKLTLTPTEKLKIRLSYMRGSSASSALS
                     SLGNNAELSETRAVGELVEPTALRDASMLYVTLSGGKGSSNNKLSLSNSCFLDGTFGQ
                     WGVAVTYTFNPATYMTLSLARSSSQTDMTRDLPRVNVNASYDPANLASGYKPPSSFGY
                     RGWLGMFYLWSDTGGTAAADLPSSLQDALTPGRLILNDPFSTPNYSTAVLGQGRYVTR
                     TFFVDTPTPAVVVSPQGYIQDGYADLSGTYYLGTGAEAVLNATADQTIVKGDITHAWR
                     AHTFKTGFEFITSELGFHAERALGLSVPGRNQNHVDYGGKYPTPHPSIFGAFAQDKYE
                     SQGLIANAGVRIERFDANYPVYVQNIFTDIALIPYGAKAYYDSIAIARGWNVSRWGGV
                     PTYYPLRDTLRKYGLGDPPEPWDIYEALPHGPNKVFWRIEPRFGVSHPVSDRTKFFFN
                     YGLFYSMQKPAVMFSFQTDERLGQVGKIQEIRYPNLRPARTTMYEVGIEHVLPHQIVW
                     TTRGYAKYNVDQVSMLYCTVYQGNRMFRNANYEEVRGLEIKVARNSGRFLNGWFTYET
                     YSTRAGEVGLYSINPNMAQSSYFTAYARSNEPLANIRGFVRLGTPRDWGSWKGGWGIG
                     VMETYREGSEVIYRPDPTIPLRELPAENYMKTVDYWYTNLKLDKTIDLQGGRTLSAYM
                     DVTNLLNTKYMSSGGMASYNDYLNYVFNRRQAGEKNLRVYDPSTEEALTRPYKDAGGN
                     WKAPISPRTEWLLYPTTRVVRFGLRFDF"
BASE COUNT         1813 a         2508 c         2769 g         1893 t
ORIGIN      
        1 aggctcgctt tcggcggacg gatggtcctt cctgatccag cccggttgca ggaaatagcc
       61 gagtggcagc gggagccctt gccggcaggc caggctgttc gcagccttgc ctcatgctgg
      121 aacgcggctg atttccttcc aacaaataca acgcggctct catggatttg gtcagtgcgc
      181 tcagtttcct gacaccacga acacgttgat gacgggtagc ttctgctttc gcggccgctc
      241 gaatgcatcc acgcatctgt tcccgggaga ttctggttac ttgttgcgtg tttgactgtt
      301 tagctatatt attcaaatgg tcaattggtc agcgggagct gcttgaagtc accgttgccg
      361 ggaggcaatc caatgaatga cacagaaatg agcggcgtgg gtgacggcac acgtgcgcct
      421 gcggacacgg gtgaaaagga caggaaaacg gaaatccgcg agaaggctcg ctctgtcttc
      481 ctccggtatg gctaccgcaa agccacgctc gatgacatcg ggaaggcctg cggcctgagc
      541 aaaactgcga tatatcacta tttcccagac aaagaagcgg tatttgccga ggtagttcgc
      601 gcggaaagtg gggttctgct ttcgaaaatg cgagccgccg ccgcatcaga aaccgacccg
      661 aaggcccgcc tcgcggaggt cattcgcacc cggttcaggg cgatgagtga aatactgaac
      721 gaagtcgtcg gcgatgcacg tcacgaggtt cgcgaatttc ttccgaaaac tgcccatgcg
      781 cgcgagcagt ttttccacga agagggaaag cttatcgaag aaatcctgga ggacggtggt
      841 cggcagggag ttttccgggt actccgaact cgcaatgggc cgctccttct catcgcggcg
      901 cttcaaggcg ttgaaaacgg tcttgccgga cttagcgact ccttgtcgat cgaagaagct
      961 attgacgaga tgctggagat tcttttcgac ggtctctgcc ggtgagccgg gcggcgaaca
     1021 ggcttcagaa gccccttttt ttacgaagat tcgaacgttt catcggtttg ttccaatagt
     1081 gagaaggagt ctaacgatgg tatggacaac tcccctgaag cgcattttgc ttgggattgt
     1141 cgcgagcctc cccgctctct gccttagtcc cctctttgca gcgtgcggca acaaggttca
     1201 aaaaaccact tccgtcgaac ccggcgtatt cgtcaagcca gtgatacgcg tcggaaacgg
     1261 gcgcgagctt catttgagcg gtacgctggc ggctgaacga tccatcgccc tcagcttcgc
     1321 cacccttgga accgtcgagc aggtgatggt ggaggaaggt caggaggtga aaaaaggcga
     1381 tgtgctcgcc aagcttgtgt cgcggagcta cgaggacgcg ctcgcgatgg cagaggcgaa
     1441 cctgaaccag gcggaggatg cataccgccg cctcgagccc atgcaccgca acggaactct
     1501 ggctgacgtg aaaatggtcg aggtcgaaac agcccgtcag caggcgcggc tggcggcgtc
     1561 catcgcgcgg aagaatctcg atgatacgag tcttcgctcg ccggtgaacg gcgtgatagc
     1621 ccaccgcaac gtcgaacccg gtgccagtgc ctcacctgtg cttcccgctt tcacgctggt
     1681 tcaggcgaaa acccttctgg caactgctcc ggtgccagag acgcaggtat cgaagatccg
     1741 gcgaggcatg agtgcacgtg tggtgattcc ggccctgcag aaaaccgtcg agggaagtgt
     1801 gcgggagatt tccgtcgtgg cgaacccgct taccagaacg tatgacctga aggtggctgt
     1861 agcgaaccct accggagagc ttcgcctcgg catgatcgcg gacgtgtaca tcaaggtcga
     1921 cggggaagtg gagaacctcg ttatcccacc cgaagcaatc cgagttgatg aggtcggcaa
     1981 cccctatgtg tacgttgtcg ccgcagaccg gaggctccaa cgccgcccgg tgagggtagg
     2041 agggtttgtc ggggagggaa cgatggtgac ggcgggcgtt cgcgccggtg aatcgctcgt
     2101 ggtatcgggc acgcccatgc tggccgacgg aatgacggtg cgggttctcg cgcctcacgc
     2161 gaacggggaa tagtccatgg atccaacgag tctgaccgaa agagccatgc gccagtggcg
     2221 aatcacagtg acggtggtcg cggtctgcgt ggtggcggga gtctattcct ttttcacgat
     2281 gcagcggcag gagtttccgg cctttaccat ccgccaggga ctggtcatcg gcgttatgcc
     2341 gggtgccacc tccgaagagg tcgaggacca acttgcccgg ccggtggagg attttctgtt
     2401 cagcttcaac gaggtcaaca aggcgaagac gtactcggtc tccaaagacg gccaacttgt
     2461 agtgttcgtt gaggtgaaag aggatcttga cgggatccaa acggccgcct tctgggcaaa
     2521 actccggcac gggctcaacg aactgaaggc ccagaaactt ccgggtggag tgctcgctct
     2581 tgtcggcaac aacgagttcg gggacaccag cgccctgctt ttcactctgg tggcggaagg
     2641 gaaaagcccg cgcgatttgc gcgaatacat gaaagtgctc gaatcccatc tgcgccggat
     2701 cgaggcaact gccaaactgc gtgtaatcgg tgagcagaac gaagtcatca gcatcaacat
     2761 ctcccgggac cgtgttgcgc gttacggtat tcggccagct actctgtggg cctctctaca
     2821 gggtttgggc gcgcttccag cctacgcccg actggacggg gatgaactgg aacgtcccat
     2881 ctacgtgagg aaggtgctgc gctccgagaa tgagcttgca gaaactatcc tcctgtccct
     2941 cccgacaggc cagcacgtgc gtttgaagga tgtggcggag atcaaacggg aatatggcca
     3001 cgacgacgcg tacgtccgct tcaacggcaa aactgcgctt gtgctgtcca tcgaaatgca
     3061 gccgggcaac gacataaccc ggttcggcga cgaggtggat cgtgcgctcg aagagacatt
     3121 gcacgagtta ccaccgggag tgacgatctc gcgcgtcgtc gaccagccca aagtggtgcg
     3181 cacatcggtc aaccacttcc tccgcgattt tggactggcc attgcctcgg tgatactcgt
     3241 gacaatgttg cttctgcccg tacgtgtggc tgcagtcgcc gcgattacta tccccgtgtc
     3301 catcgcgatc actcttgcca tcctgaacgc gctgggagtt gagcttcaaa ccgtctcgct
     3361 cgccgggctt attgtggtgc tgggaatgat cgtagataac gccattgtgg ttattgatga
     3421 ccacgtggac aagcttgatc gcggcatgga tgcctggtca gctgcctgga tgagcgcgca
     3481 tgatctcgcg gtgccggttt tcacagcaac gatcgcgatt attctgtctt acgttcccat
     3541 gccgcggttc ctgaccgggg ttgctgcgga attcctcgga agcttgcccg tgacgatcgc
     3601 agtggcactt tccgtcagcc tgctgatcgc actcctgctc cttccaatca tgaactctca
     3661 gttcgttcgc agggggttac gcaaacggag cggccagcga tccatgctcg agcggatcca
     3721 atgcgtgttc gacagggctc tggatgcggt attccgtcat ccatggctca ccgtagcggg
     3781 cgggattggc tcggttctca tcgcggtcgt gatcgcgctt cgcattccgc agcagatgtt
     3841 ccccaaggtg gaccgtgatc agtttgccgt agaggtgtat ttgcccactg gccgcagtct
     3901 gaagcaaacg gacgcagtca tgcggcggct cgaggccgaa ctccttgcgg ataagcgggt
     3961 ggtcaacgtt acctcattcg tcgggcaaag cgcgcctcgt ttccacacgc tgtatgcgcc
     4021 ctacataccg aggcggaatt tcgggcaact cctcgtcaac accattgacg ccgatgcgac
     4081 gctggacgtt ctccgcgaaa gtgaagaacg gctgcggggg gccttcccgg agtgctgggt
     4141 gcgctggaag caactcgaat tccaggtcgg ttttcccgtg gaagtccgtc tatcgggcaa
     4201 cgacgcggaa gcgctcaaaa ccaccggggc gaagatagag tcgtacgcac gcggaattgc
     4261 cgaagtaacc tgggtgcaca acgactggga agaagcgctg caatctgttg acgttatgcc
     4321 ggacgcggac gcgtgcgccc gtctgggcgt accccccgcc atgctccagg cgtcgctcgc
     4381 tctgggcaca caggcgtacc gggtgggaac gatctgggag ggtgattacc ccgtgcgtgt
     4441 tcttgtgcgg gacgactctg gagaacgcgg aagcgttgag gggctgcgcc agcaatacgt
     4501 ttcttcgatt ctcggcgcga ccgtgccact ggagcaaatc gcttcgttga gaccggcgtg
     4561 gcatgagggc gccatggtcc gtcggaatgg tgttcgcacc ctcacggtct tcatcgacat
     4621 aaaaaaggac gcgctggctt cggaaatgca agagaaaatt gaaaaatacg tctctgggct
     4681 cgacctgcca ggcatccgtg tgagttacgg tggtgagaaa gagctttcca cgcagatctt
     4741 ctccccgatg acgatttcga tgttggtaag cattggactc attttcctcg tggtgctgtg
     4801 ccagttccaa cgcttcctca ccacccttgt cgtgatgctt tcgatgccgc tgaccctcat
     4861 gggtgctttt cttggcctgg tgatcatggg gtatcccttc gggttcactg ccttcgttgg
     4921 gataattgga ctgatgggca tcgccgtccg caacgggatt atcctggtta cctatgcaga
     4981 agaactccgc gtgaagcaag gactgagcgc gatggaggcc gcacgagccg ccggcaagcg
     5041 gcgcatgcgc ccgatctacc ttacatcgac tgccgcggcg gtcggagtgg tgccgatgat
     5101 tctcagccgg tccacgttgt ggggccctct cggaactgtg acattcttcg gattgcttac
     5161 tgcgatggtg ctgacattat tcgttttgcc ggtgacgtac gcgcttgtgg tcggcaggga
     5221 agaccgccgg cacgagcctt cgccaaaggc tcctgtggtg acgggattgc ttcttgggct
     5281 tgctctggca gtctccccgg cgagcgctga cgaaacaccg ctcacgcttc aggaatgcaa
     5341 ggaactcgcc ctccagaaca acgcccagat cagggaatcg aacctcgaaa tagaagcggc
     5401 ccgcgaaact cggcgagcag cctacacgaa atacttccct cagatatcgg tatccgccac
     5461 cggggcggtc gcggtggatc cgctggcaag gatacatacg agcgggggca acctccctgt
     5521 gtacaatggc aatcctgcaa ccatccccac ggccactcag ttcgcatact ttccgagcgg
     5581 ggaaatggcg atcgcgggcc gcggtttcgt ggcttcggcg gcagtaattc aaccgctgtt
     5641 tgcaggcggg cggatcgtga gcggaaaccg cctcgctgac ctcggggtcc acgttgcgaa
     5701 agacagggcg gaaatctcca agcgcgatgt actggcgcag acggaggaga agtactggcg
     5761 gctcgttgcc cttcgggaaa agcttcggac gctcgacgcg tacgaggcac tgctcgaggc
     5821 gctggacagg caggtcactg atgccgtgca ggcggggctc ttgactcgca atgatcagct
     5881 caaagtccga ctcaagcgtt ctgaagcgag cgtcgatcgt caacgactcg aaaacggtgt
     5941 ggcgttggcg acgcgggatc tgcgccgcca tatcgggttg ccggagggcg gtgcgcttcg
     6001 ccttgtcgac actctggcgt gtcctgacga cccatctatt cttgcgcagg agcgagccgg
     6061 agcactcgat cgacgtcccg aaattcgtct gctctcccgc gccattcgcg ctgagcagct
     6121 tcagaactcc ctcacgcgtg gggggatgct acccacggtt tccgtcggtg caatagggtt
     6181 gcggcaggat atccgcggaa tgatcagcca gaccaacggt ctgctgatgg gaatggtaaa
     6241 cgttccaata tccggtttgt gggaggggag gcacacaacg gccagccaac acaggcgggt
     6301 agagattgcc gaaaatcggc ttgctgagac gcgcgagttg ctggacttgc aaatcgagaa
     6361 cgcgtggacg gatctgacgg cggcctggcg ctccaatgag gtggcgaacg aggccgtaga
     6421 tcaggcggac gtgaacctgg ccgaggtgtc ggatcggtac cggaacgggc ttgtcacgtt
     6481 ctccgacctt ctggaagcgc aggtgctccg ccagaaagcc acggacagcc gcatcgacgc
     6541 acgggcagac tactggttga aacgcgccgc ttaccttcga gccgtcgccg ccgccgacgg
     6601 atcagagtga gtatgaaaaa ggcctggccg aaaagtactc ggccaggcct tttccttgtg
     6661 tcttgtttgc tcgcttagaa gtcaaatctc agtccgaacc gcacgactcg tgttgtcggg
     6721 tagagcagcc attccgtgcg cgggctgatg ggtgccttcc agttgccccc ggcatctttg
     6781 tagggtcttg taagggcctc ttccgtcgag ggatcataca cccgcaggtt cttctccccc
     6841 gcctgccggc ggttaaacac gtagttcagg tagtcgttgt aactcgccat tccccctgaa
     6901 ctcatgtatt tcgtgttcaa aaggttcgtc acatccatgt acgcggagag tgtcctgccg
     6961 ccttgaaggt cgatggtttt gtccaacttg agattggtat accagtaatc aacggtcttc
     7021 atgtagttct cggccggcag ttcacgaagc gggatcgtcg ggtcaggacg atagataacc
     7081 tcggatcctt cgcggtaggt ttccatcacg ccgatacccc atccgccttt ccatgagccc
     7141 caatcgcgtg gggtgcccag acgaacgaaa ccgcgtatgt tcgccagcgg ttcgttggag
     7201 cgcgcatacg cggtgaaata actcgactgt gccatgtttg gattgatgct gtacagacct
     7261 acttcgccgg cccgcgtcga gtaggtttcg tacgtgaacc agccgttcag aaacctccct
     7321 gagtttcgtg caaccttgat ctccagacca cgcacctcct cgtaatttgc gttgcggaac
     7381 atgcggttgc cctggtaaac ggtgcagtaa agcatagaga cctggtccac gttgtacttg
     7441 gcgtagccgc gggtcgtcca gacgatctgg tggggcagga catgctcgat cccaacctcg
     7501 tacatcgtgg ttcgtgcggg gcgaaggttc ggatatcgga tctcctgtat cttgccaact
     7561 tgcccgaggc gttcatccgt ctggaagctg aacatcactg cgggcttctg catcgaatag
     7621 aagaggccgt agttgaagaa gaatttcgta cggtcgctca ccgggtgtga tacaccgaac
     7681 cgcggttcga tgcgccagaa caccttgttg ggcccgtggg gaagggcctc gtagatatcc
     7741 cacggttcgg gtggatcgcc aaggccatat ttgcgcagag tatcacgcaa cgggtagtac
     7801 gtcggaactc cgccccaccg gctcacgttc caaccgcgtg cgatggcgat gctgtcgtag
     7861 tacgccttgg ctccgtacgg aatgagggcg atatccgtga agatattctg gacatatacg
     7921 gggtagttgg catcgaagcg ctcgattcgt accccggcgt tggcgatgag cccctgggac
     7981 tcgtacttgt cctgcgcaaa agcgccgaag atgctggggt gaggcgtggg gtactttccg
     8041 ccatagtcca catggttttg atttcggccg ggaacggaga gaccgagcgc ccgctccgcg
     8101 tggaagccaa gttcacttgt gatgaactca aaaccggtct tgaatgtgtg agcgcgccac
     8161 gcgtgggtaa tatcgccttt cacgatcgtt tggtccgcag tcgcgttgag cactgcttcc
     8221 gcacctgtcc cgagatagta tgtgccgctc aggtcggcgt atccgtcctg aatgtatccc
     8281 tgcggagaca ctaccacggc aggagtcggt gtatcgacga agaaggttcg tgtcacgtaa
     8341 cgcccttgcc ccagcactgc ggtggaatag ttgggcgtgg aaaacgggtc gttcaaaatg
     8401 agccggcccg gggtcagcgc gtcttgaagg ctgctgggca gatccgcggc ggcagtgccg
     8461 ccggtatcgg accagaggta gaacattcca agccagcccc ggtacccgaa cgaacttggc
     8521 ggtttgtacc cggaggccag gttcgcgggg tcgtacgacg cattcacgtt gacacgggga
     8581 aggtcccggg tcatgtcggt ctgcgaactg gaacgagcga gggagagagt catatacgtg
     8641 gccggattga acgtgtaggt gacagcaact ccccattgac cgaacgttcc gtcgaggaaa
     8701 caggaattcg aaagactgag tttattgtta ctcgagccct tgcctcccga aagggtcaca
     8761 tataacatgc tggcatcgcg cagcgccgtc ggttccacaa gctcgccaac agctcttgtt
     8821 tcggagagct ccgcattgtt gccgagggaa gacaatgccg acgaagcgct cgaccctcgc
     8881 atgtaggaga gtcgtatctt gagcttctcc gtgggggtga gggtgagctt catgtcgaag
     8941 gtctgatcgc ggaaataggg acgcaatggc ggggtaatag tca
//
LOCUS       DAOSOO010000129         8822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7911_length_8822_cov_6.043801, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000129 DAOSOO010000000
VERSION     DAOSOO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7911_length_8822_cov_6.043801"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1011)
                     /locus_tag="PLT86_09610"
     CDS             complement(76..1011)
                     /locus_tag="PLT86_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT36909.1"
                     /translation="MQSPDRRELREDVTDAIWEPVFDEAAPTFDADTPKRLKRDRPKW
                     WLHGLLFGLTAWTCYRAAGWYYSIGLMAILLAHEMGHYIMSLRWRVRATPPFFLPFPD
                     IPGVIESPLGTLGAVISMKSPVPSKRALLDIGATGPISGMVVSLVVVVVGLHFSTIEV
                     VPDRLPAGTGLYVFGDSLLFSALRYAILGPVPAGYDVFLHPLARAGWVGFFVTALNLL
                     PIGQLDGGHVLYALHQKHYRLVVRLVTAALIALILVSPVWILFALMAGIFGQTHPPPL
                     DSYTPVDRKRRILGYLCAAILVACFMPDPIDLKVF"
     gene            complement(1038..2327)
                     /locus_tag="PLT86_09615"
     CDS             complement(1038..2327)
                     /locus_tag="PLT86_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36910.1"
                     /translation="MKIVLIGAGSASFGRGQVVDVLSAAELQGKHVELCLVDVRQAAL
                     DTMTRFAERVKAHIGSDVIVTATGNRRAALSGADYVMLAVARERAKLWEQDFRVPLAY
                     GFRHCLGENGGPGAVFHALRSFELIIPICHDMEELCPNAWLFNFTNPESRVLHAMKHL
                     TNVKGAGFCHGVFTVLEYLTRILNRPLDDLEVVSAGINHFYCVLRLCDRNTGADLLPH
                     VIQRAASDRNAPPLFRKIAEVFGVITFPSDDHIGEYLSFGTEFSGVKWPYGWESKPVP
                     PVPPTGGPPALEDYASGKVPLDAEILRTSGELTVPAICDMELDRKSSRPAVNVLNTEA
                     YVENLPRTAVVEVPATIGREGITPFHVGAIPEPFASYMRAQTTIVELVTEAYRTKSRR
                     LLLQALLLDPVVNSVSAAERMLDDMLSLQRDYLPRFD"
     gene            complement(2324..3475)
                     /locus_tag="PLT86_09620"
     CDS             complement(2324..3475)
                     /locus_tag="PLT86_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HOT36911.1"
                     /translation="MPVGSVFQSELREFTDALSGTRIHQLTNYRGHSHHLYFTNPGWY
                     DNGRKLLISSDRMNRTNLFGIDLETGLLTQLTDYDPLPPPHEVEFLRCCVNPVRDEAY
                     YFYGRDVVALDLITGAQRTIWSLAAGFVPSQINCTADGARVVLGIHEDLSARMRVDYL
                     RGYVGFRETWAAKPLSRIIAIATDGSRSDTVWEERYWIGHTNASPTQSNLITFCHEGP
                     WELVDCRIWGLDLESGCVWKIRPRRVEGEAVGHEHWLANGVHVAYHGSLPDGNKCMGV
                     IRFDNQNCVEYAFPYETGHVHSNDFRLVVGDAGDVVRIWRLEDQGFRPPRVLCRHRCS
                     SHIQQLHVHPRFNRAGTQVLFTSDMSGYGNVYLADVPDVGKLPDVEVLR"
     gene            3650..4720
                     /gene="hydE"
                     /locus_tag="PLT86_09625"
     CDS             3650..4720
                     /gene="hydE"
                     /locus_tag="PLT86_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOT36912.1"
                     /translation="MSGVPRLTRGDILRWLKESDRVRLAQLWEWADAVRHEFVGDEVH
                     LRGLIEFSNYCSRQCAYCGLNARNADVVRYRMSADEILACAHQAVGFGYGTVVLQSGE
                     DYGVTREWLGEVVRRIKAETPLAVTLSVGERTEAELAYWKDCGADRYLLRFETSNRAL
                     YDRIHPPRAGIHSDRPALLRYLRSIGYEIGSGVMIGIPGQTYEDLANDIELFRELDLD
                     MIGVGPFLPHEKTPLGKEEEQLWAPLGEQVPNSELMTYKVVALTRLVCPYANIPSTTA
                     LATVNTKNGRELGLKRGANIVMPNVTPPQYREKYEIYPAKACIRETADMCHQCMKHRI
                     ESIGRQIGKGRGDSPNRTTRRD"
     gene            complement(4739..4816)
                     /locus_tag="PLT86_09630"
     tRNA            complement(4739..4816)
                     /locus_tag="PLT86_09630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4779..4781),aa:Pro,seq:ggg)
     gene            complement(4843..4920)
                     /locus_tag="PLT86_09635"
     tRNA            complement(4843..4920)
                     /locus_tag="PLT86_09635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4883..4885),aa:Arg,seq:ccg)
     gene            complement(5055..5363)
                     /locus_tag="PLT86_09640"
     CDS             complement(5055..5363)
                     /locus_tag="PLT86_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT36913.1"
                     /translation="MSDQSNLTKAELAQTIADQVGLTATATQAVIDGFLAAVVLALEE
                     HRSVQLRGFGTFTIIKRAPRVGRNPRTKERVPVPERAAPVFRPCRELRQAVASHKKEG
                     "
     gene            complement(5373..6257)
                     /gene="sppA"
                     /locus_tag="PLT86_09645"
     CDS             complement(5373..6257)
                     /gene="sppA"
                     /locus_tag="PLT86_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT36914.1"
                     /translation="MPALTGKSRRDQIIDGIVVGLIALFVLMILAVAAGSFSARKLVP
                     GERIGLVELTGAITDPEPVNRQLRHFRDVEKVPAILLRIDSPGGYVGASQEIYRQVEL
                     IRQRGIKVIASMGNVAASGAYYVAIAADTIMANPGTITGSIGVIAEFPQADTLLRKVG
                     LAFNIVKSGRYKDTGSPTRPMRGDEQELLQSVVDDTYLQFREAVGKARNIPQARLDSL
                     TQGQVFTGRMAKAFGLVDTLGNYDDALRLAKRMAGLSDGAKVIRAPSAEKSLFQRLLG
                     NVNDLWHASSWSVSYRLP"
     gene            7298..>8822
                     /locus_tag="PLT86_09650"
     CDS             7298..>8822
                     /locus_tag="PLT86_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36915.1"
                     /translation="MLRSRVVLFLLLTLATSGLVSAARATDVSGTITTTKWTKANSPY
                     RVVGAIAVPPGNTLTIEPGVDVLFDSTAQILVSGRLVAIGTETDSIRFLMGTTDWRGI
                     RISGGDSSTLAYVRISGSISTVFSGPNGAGIGITGANTRLGISHSVISGNQNGMSGGG
                     IAVGDSARLTMRRCAIRDNYCYYAGGGIYNKGDAALSECLISGNVPSGLFYGGGIYNT
                     GTLAAKGCTITANRAGNGGAGIYNESGGTVEIDSSVISSNRAGIAGGGFGNYGTMRVT
                     RCLVINNLANGDWPEGAGGRNGGSLYAANCTFYGNSGGSGNGITHSGGTTELVNTIIW
                     GNAAPQLKGFDQPGVTVTYSNIEQDAGLFPGTGNINADPQFTDAANQDLRLWPGSPCI
                     DTGDPTSPLDPDGSRADMGARPFDPTLTVESPQMPPRFLVMQNAPNPFNPSTTIRFTL
                     PEAGYVTLAVYDINGRVVRNLAEGPLPAGVHDVVWDGYDAMGRAVASGVYVYRLTAPQ
                     "
BASE COUNT         1819 a         2680 c         2648 g         1675 t
ORIGIN      
        1 tctcggcggg cgctcatttg gctggccgcc cggctgaacg gccgaccccg ggtgctgccg
       61 tgccattcca gcctgtcaga agactttgag gtcgataggg tcgggcatga aacacgctac
      121 aaggatggca gcgcacagat aacccagaat gcgcctcttc ctgtcaacag gtgtgtacga
      181 atcaagcgga ggtggatgcg tctgcccgaa gattcccgcc atcagggcaa aaagaatcca
      241 gacgggcgac acaaggatga gggcgatcaa ggcggcggtg acgaggcgca cgaccaggcg
      301 atagtgtttc tggtggagcg cgtagagtac gtgcccgccg tctagctgcc cgataggaag
      361 aaggttcagc gcggtaacga aaaagcccac ccacccggcg cgggcaaggg gatggaggaa
      421 cacgtcgtac cccgcgggaa ccggcccgag tatcgcgtac ctcagtgcgg aaaaaagcag
      481 cgaatcgcca aacacgtaca agccggtacc tgccggaagc cggtcaggaa caacctcaat
      541 cgttgaaaaa tggagcccga ccaccaccac tacgagtgac accaccattc cggagattgg
      601 accggttgcg ccgatatcca gaagggcacg cttgctcggg acggggcttt tcatggaaat
      661 cacggcgcca agcgttccga gcggggattc aattacgccc ggaatgtccg ggaatgggag
      721 aaaaaaggga ggagtcgccc gcacgcgcca cctgagtgac atgatgtagt gccccatctc
      781 atgcgcgagg aggattgcca tcaatccgat gctgtaatac catccggccg cgcgatagca
      841 tgtccatgcg gtaagaccaa atagaaggcc atgcagccac cacttaggcc gatcccgttt
      901 gagacgcttc ggtgtgtctg catcaaacgt gggagccgcc tcatcgaaaa caggctccca
      961 gatggcgtcg gtgacgtctt cccgcagttc gcgccgatcg ggcgattgca taacaacgcg
     1021 cccttcttcc ccgttgacta gtcaaaacgc gggaggtagt cccgctgcaa ggacagcatg
     1081 tcatccagca ttcgttccgc cgcggacaca ctgttcacca ccgggtcgag caacagcgcc
     1141 tgaagcaaca gcctgcggga tttcgtccgg tacgcttcgg tcaccagttc cacgatcgtt
     1201 gtctgggccc gcatatagct ggcgaacggt tccggaatgg ctccgacgtg aaacggcgtg
     1261 ataccttcac gaccgatggt tgccggcact tcgacaaccg ctgttcgcgg caggttctcc
     1321 acgtaagctt ctgtgttcaa cacattgacc gccggtcggg aggattttct gtcgagttcc
     1381 atgtcgcaga ttgcaggaac ggtaagttcc cctgatgttc gaagaatctc cgcatcaagg
     1441 ggcactttgc ccgatgcgta gtcctctagc gccggtggcc ccccggtcgg tggcacggga
     1501 ggaactggtt ttgattccca cccgtagggc catttgacac cgctgaactc ggtgccgaag
     1561 gacagatact cgccgatatg gtcatcggac gggaaggtga tcaccccgaa cacttccgcg
     1621 attttgcgga acaacggcgg cgcattccga tccgatgcgg cccgttggat cacatggggc
     1681 agcagatcag caccggtgtt tctgtcacaa agtctcagaa cgcagtagaa gtggttgatt
     1741 ccggctgata ctacttccag atcatcgagc gggcgattga gtatccgggt caggtactca
     1801 aggacggtga acactccgtg gcagaacccg gcgcctttta cgttggtcag atgtttcatt
     1861 gcatgcagga cacgggattc cgggttggtg aaattgaaca gccacgcgtt cggacaaagc
     1921 tcttccatgt cgtgacagat cgggatgatc aattcaaagc tccgcaacgc gtggaacacc
     1981 gcgcccggtc ctccgttctc gcccagacag tgtcggaagc cataggccag cggaacgcgg
     2041 aagtcctgtt cccagagctt tgcacgctcc cgggcaacgg caagcatcac atagtcggct
     2101 ccggacaggg ctgcgcggcg attaccagtg gccgtaacga tcacgtcgct cccgatgtgc
     2161 gctttcacac gctccgcgaa gcgcgtcatt gtgtcgaggg ctgcctgacg cacgtctacg
     2221 agacagagct caacgtgttt tccctgcaat tccgcggcag acagcacgtc aaccacctga
     2281 ccgcgaccga acgaggcgct tcctgcaccg atgagaacga ttttcaacgg agaacctcca
     2341 catcggggag tttgccgaca tctggcacgt cagccaggta cacgtttccg taacccgaca
     2401 tgtcgcttgt gaaaagcacc tgtgtgcccg cgcggttgaa cctcgggtgc acgtggagct
     2461 gctggatgtg gctgctgcaa cggtggcggc atagaacccg cggcggcctg aaaccctggt
     2521 cctcgagtcg ccagatgcga accacgtcgc cggcatctcc gacgaccaac cggaagtcgt
     2581 tggagtgaac gtggcctgtc tcgtacggga aggcgtactc gacgcagttc tgattatcga
     2641 atcgaatcac gcccatacac ttgttgccgt cggggagaga accgtggtaa gcaacatgca
     2701 cgccgttggc aagccagtgt tcgtgcccca ctgcttcgcc ttccaccctg cgggggcgta
     2761 tcttccacac gcagcccgat tcgaggtcca agccccatat ccggcagtct acaagctccc
     2821 aggggccttc gtggcagaac gtgatgaggt tggactgggt gggcgatgcg ttggtatgcc
     2881 cgatccagta gcgttcctcc cacacggtgt cagatcggga accgtcggtt gcgatggcga
     2941 tgattctgct caacggtttg gccgcccagg tctctctgaa gccgacgtag ccccgaaggt
     3001 aatcgacccg cattcgagcg gaaagatcct catggattcc cagtacgact cgtgctccgt
     3061 ccgcagtgca gtttatctgt gacggaacaa agcccgccgc aagtgaccag attgtccgtt
     3121 gtgcacccgt gatgaggtcg agggctacca cgtcccggcc gtagaaatag taggcttcgt
     3181 ctcggacggg gttcacgcag caacggagga actcaacctc atggggtggc ggcaaagggt
     3241 cgtagtcggt cagttgtgtg agaagaccgg tttccaggtc tatgccgaaa aggttggtcc
     3301 gattcatgcg gtcggagctg atgaggagct tccttccgtt gtcgtaccat ccgggattcg
     3361 tgaagtagag gtggtgactg tgaccgcggt agtttgtgag ctggtgaatg cgcgtgccgg
     3421 acagagcatc agtgaattcg cgcaattcag actggaatac cgaacctaca ggcattcttc
     3481 tccttttcta tcctggggaa acactctggt cgaaagaggt gcaatcaaaa cataacgggc
     3541 gccctgaaac ggacaaatgc ccgcggaaag ggctgcgggg aaccaaaact gcaagaagga
     3601 tgtattgttc gtgtgagaag cagggagaca tgatgaacag gagaggacca tgagtggagt
     3661 gccgaggttg actcggggcg atattcttcg ctggctgaaa gaaagcgatc gtgtgcggct
     3721 tgcgcagctc tgggaatggg ccgatgcggt gcggcatgag tttgtcggag atgaggttca
     3781 cttgcgagga ttgatcgagt tttccaacta ctgctcccgc cagtgcgcat attgcgggct
     3841 gaacgcgcgg aacgccgacg tcgtccgata ccgcatgagt gcggatgaaa tccttgcgtg
     3901 tgcccaccag gcagtcgggt tcgggtacgg aaccgtggtt ctccagtcag gcgaagatta
     3961 cggagttacc agggaatggc tgggagaagt agtgcgtcgg ataaaagcgg agacgccgct
     4021 ggccgtaact ctgagtgtag gggagcgaac ggaagcagaa ctggcatatt ggaaagattg
     4081 cggcgcggac cgctatctcc tgcgatttga aacgtccaac cgtgcgttgt acgaccgtat
     4141 ccatcctccg cgcgcgggca tccattccga ccgtcccgcc ttgctccggt acctgcggag
     4201 catcggctac gaaatcggga gcggcgtgat gataggtatt cccggccaga catatgagga
     4261 ccttgccaac gacatcgagc tgttccggga actggacctg gacatgattg gcgtcgggcc
     4321 gtttctgccc cacgagaaaa ctccgctcgg caaggaggaa gagcaactct gggcgcctct
     4381 tggagagcag gttcccaaca gcgagttgat gacgtacaag gtggttgccc ttacccggct
     4441 ggtgtgtccg tatgcgaaca ttccgagcac taccgcgttg gcgacggtga acacgaagaa
     4501 cgggcgggaa ctcggtctca aacgaggggc caacatcgtg atgccgaatg tcacgccgcc
     4561 gcaataccga gagaaatacg agatctaccc ggccaaagcc tgcatccgcg aaacggcaga
     4621 catgtgtcac cagtgcatga aacaccggat cgaatcgata ggcaggcaga tcggcaaagg
     4681 gcggggcgat tcacccaaca ggacaacccg cagggattga attccaaagg ggggaaaatg
     4741 gtcggggcgc ccggattcga accggggacc accagacccc cagtcaggtg cgctaaccgg
     4801 actgcgctac gccccgaaca tcaaaccagt gaacaagggg gatggtgagc taggcaggat
     4861 tcgaacctgc gacctttggt tccggagacc aacgctctat ccaggctgag ctactagccc
     4921 tcccgtctca ctaccgcaac ttcttcttat gacggttctt ccgaagccgt tttttccgct
     4981 tgtgcgtggc gatcttatgc cgctttcgtt tctttccgca aggcaatggc atctctcctg
     5041 agaacgtggt gtgttcagcc ttccttcttg tggcttgcta ctgcctgccg taattcccgg
     5101 cagggccgga aaaccggtgc tgctcgttcg ggaacgggaa cccgttcctt cgttcgcggg
     5161 tttctcccga ctcggggcgc ccgcttgata atggtaaatg taccgaatcc gcgcaactga
     5221 acgctgcggt gctcctccag cgccagcacg accgccgcaa gaaacccatc gatgacagcc
     5281 tgggtcgcgg ttgccgtcag ccccacctga tccgcgatgg tctgcgccag ttcagccttc
     5341 gtaaggttgc tctgatcgct catatacgcc tcctatggca aacgatagga gaccgaccat
     5401 gaggacgcat gccacaggtc gttcacgttt ccaagcagcc tttgaaacag gctcttctct
     5461 gctgacggtg ccctgataac cttcgctccg tcgctcaggc ctgccatccg tttcgcaagg
     5521 cgcagggcgt cgtcgtaatt ccccagcgtg tctaccaggc cgaacgcctt cgccatgcgg
     5581 cccgtgaaaa cctgtccctg cgtcagcgag tccaaccgcg cctgcgggat gtttctcgct
     5641 tttcccaccg cttcccggaa ctgcagatac gtatcgtcaa ccacggactg gagcaattcc
     5701 tgttcatcgc cccgcatcgg gcgggtgggc gaacctgtat ccttgtagcg gccgctcttg
     5761 acgatgttga acgccagccc caccttgcgc agaagagtat ccgcctgcgg aaactctgcg
     5821 atcacgccga tggacccggt gatggtgccg gggttcgcca tgatggtatc cgcggcgatg
     5881 gcgacgtaat atgccccgct tgccgccacg ttgcccatcg aggcgattac tttgatcccg
     5941 cgctgccgga tgagctcgac ctgccggtat atttcctgcg acgccccgac atacccgcca
     6001 gggctgtcaa tgcgcaacag aatcgccgga accttctcga catcgcgaaa atgccgcaac
     6061 tgccgattga ccggctcagg atccgtgatg gcgcctgtta actcgacaag gccaatccgt
     6121 tcgccgggca ccagtttccg ggcgctgaag ctgccggcgg ccacggcgag aatcatcagc
     6181 acgaatagcg cgatgaggcc caccacgatg ccgtcaatga tctgatcacg acgacttttt
     6241 ccggtcaatg cgggcatcag gaagcctcac gggtgttcgg caaatgagat ttacaaatat
     6301 acccccgcat ggggcaatcg tcaacgcaag cccgtagccc ggaagcccga tcatcgagtg
     6361 gcgcggcgga gcaacgcgac gagctgtatc gagatgacca taccacctcc ctcatcacct
     6421 cgcattcgct ggcgcgaacg tttcccttct tcccccaatg gtggacgagg ggaccagcga
     6481 cgtgccggcc agataacctt acgcgcaaca caaccgtgtg gaggggcacg ctccgtcgtg
     6541 cccgtattct ctcccgcgca ccaattcgtc gacgggcgcg cgcgaaatcc ctccccacac
     6601 actagctaag gcaaaacatc gtcttcgtgt caagcctttt cgtgtagaag ctgcaaattt
     6661 gcaccaggat tttttttcga aggttaagcc gaggacggtc tccgagtggc gggaggacga
     6721 agtccgagcg gcgtatcgag gagcccggcc cggcggttca ccttccgtcg gctcgtatgt
     6781 aacgagcctg cgcgtctcga tacgtctccc tcgcatgtgc tcgtgagcca ctcgacgacc
     6841 gtccggcggg cgcccgaaca gcggtcatcg agtggcgtcc gccggacggt cggcggacgg
     6901 tgtatcgaga tgaccgcaaa agccgcgccc ggccacccgg tctggattcc cgcctccgcg
     6961 ggaatgacgg attgtgggcg tgttcctgac cctctggcaa agaaaaagtc aatgactttc
     7021 cgttcgtcat ttccgacagc gcaggtgtat gctgatcatc ccgaagaagc gaacgtcatt
     7081 cccgcgcagg cgggaatcca gcccagtgcg aatcgccctg cgaagctgtt ccccgccctc
     7141 gccacttgcc cccccccgga ataactacat tacccggcga tcaacacacc gcacatcgtt
     7201 gacaaccggt gctgaccggg ctacattcaa cgcattgtga tgtgcgctca aggcatcggc
     7261 tggcagggcg cagacgccct gctgagggag gtgcgccatg ctgcggtctc gcgttgttct
     7321 tttcctcctg ctcaccctcg ccacaagcgg ccttgtcagc gccgcccggg ccaccgatgt
     7381 gtccggcacc atcaccacga cgaagtggac gaaggccaac agcccgtacc gcgtggtggg
     7441 cgccattgcc gttccacccg gcaacaccct caccattgaa cccggtgtcg acgtgctgtt
     7501 cgactccacc gcgcagattc tcgtaagcgg ccgcctcgtt gccatcggca ccgaaacgga
     7561 ctccatccgg ttcctgatgg gcacaactga ctggcggggg atccgcatct ccggcggcga
     7621 ttcgagcacg cttgcgtatg ttcgaatcag cggctccatt tccacagtgt tcagcggccc
     7681 aaacggcgcg gggatcggga tcacgggggc caacacgcgt ctcggtatct ctcattccgt
     7741 catctcgggc aaccagaacg ggatgtccgg cggcgggatt gcggtggggg acagcgcccg
     7801 gctcaccatg cggcgctgcg cgatccggga caactactgc tactacgccg ggggaggaat
     7861 ctacaacaag ggcgacgcag ctctctcaga atgcctcatc agcggcaacg tgccctccgg
     7921 ccttttctac ggcggcggca tctacaacac cggtacgctg gcggccaaag ggtgcaccat
     7981 taccgcaaac cgcgccggca acgggggagc cggtatctat aacgagtccg gcggtacggt
     8041 ggagatcgat agcagcgtca tttcctccaa tcgagccggg atcgccgggg gtggtttcgg
     8101 aaactacggc accatgcggg tgacgcggtg cctcgtgatc aacaacctcg cgaacgggga
     8161 ctggcccgaa ggggcgggcg ggcggaacgg cggctccctc tacgcggcca actgcacctt
     8221 ctacggtaac agcggcggca gcggaaatgg gatcacgcat tccggcggca ccacggagct
     8281 ggtgaacacg atcatctggg ggaacgcggc cccgcaactc aaggggttcg accagcccgg
     8341 cgtcaccgtg acctactcca acatcgagca ggatgcgggg ttgttccccg gcacgggcaa
     8401 catcaacgcg gacccccagt tcaccgatgc ggcgaaccag gacctgcgcc tatggcccgg
     8461 ctccccgtgc atcgacacgg gcgacccgac gtcgccgctt gacccggacg gctcccgcgc
     8521 ggatatgggc gcgcggcctt ttgatccgac gctgactgtc gagagccccc aaatgccgcc
     8581 ccgtttcctc gtcatgcaga acgcgcccaa tcccttcaac ccgagcacca ccattcgctt
     8641 caccctgccg gaggcggggt acgtgacgct ggcggtgtat gacatcaacg gccgcgtcgt
     8701 gcgcaatctg gcggaagggc ctttgccggc cggcgtgcat gacgtcgtat gggacggata
     8761 tgacgctatg ggtcgcgcgg ttgccagcgg ggtgtatgtc taccggctca ccgcgccgca
     8821 gg
//
LOCUS       DAOSOO010000130         8815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7921_length_8815_cov_5.544635, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000130 DAOSOO010000000
VERSION     DAOSOO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7921_length_8815_cov_5.544635"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLT86_09655"
     CDS             complement(<1..1128)
                     /locus_tag="PLT86_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT36916.1"
                     /translation="MAKRRNLEQVFEALEDGIVAVTKDGQIFHTNTAAVRLLGGNLRS
                     HTGENIDEILSERCGREVRLLQRAIGSGKPSSPERLQLRAGENGFFSVIARVQLLSDE
                     TGLLGAVVLLRDETEISRLRANLEGARGLENIVGASEPMQQVFQLVREVARTDATVLI
                     QGETGTGKELVARAIHQLSNRADKPLVTVNCAALPEGLLESELFGHVKGAFTGALSDR
                     LGRFEAADGGTLFLDEVAEIPPMIQVKLLRVLQERVFERVGENKPRRTDARLISATNK
                     NLSDMVSEGRFREDLFYRLNVFPIHVPSLRERVDDIPLLVHRLLSRIADRHGTAVPGI
                     SDDVLRLFRSYRWPGNVRELQAALEYAMIRSQGATIDVHHLP"
     gene            complement(1208..4375)
                     /locus_tag="PLT86_09660"
     CDS             complement(1208..4375)
                     /locus_tag="PLT86_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36917.1"
                     /translation="MHFFAEGTPTDMAPFAERMAWTPRFDSGLQWCEARDIERVVVHW
                     RDDVPAATDVRLEYWKGQWPEKRVPKGQVTGGGFPGWMAEDDWFNGRWQVADTVLAAA
                     DKQWIFTFNPLNTREFAEMRDFAARFRHTLRIRLVSDKILPAIISWEAYTDSTWFRRD
                     IVVERETRGQSAEKESTWLVEVCNGHLASQKPSPKLADAAGEGCAKLLRQHKAGSELV
                     RFGVWTTRNEDPTTRDHTVVTVRTGEATFSFAANDLDQGPIHLEDFGVLVSETRHKAT
                     LRQFRRTRPKSRTYYDRVFEMPEQTLARAWQDMPAKRPFYFVLGVEGRRQRFGVEPNG
                     DVFRSHERQWFEKMPGRETERVKEEPNAVRWSFGLPAEPPTQRVPLRGYLPVMTTSWE
                     TDGLRYEQTAFATLLDRSLDDTPIRGDDPVVAIVLVKATNTSNVEKDGTLTLRVAAGD
                     AQERLQLARHGYVRNQHGKLRCVITHRKGRLSRTTEGVHYSLRLRPSECTTIAFKLPF
                     FGLNEAELGLLEVIQPQSALQEVTDYWERRIAAGTRIVTPEPLINDFYRAHITHLLIN
                     HERHPASPVSGGHELDIARVGSSRYGAYTNESVMQLSDLDRRGYSQEAANGYELFLHF
                     QGTVPLPGDFTSHRGVLYGAGGSEHGNYNQNHGWALWGLAEHYFLTRDRDWLHRVAGK
                     IVEACDWITEQRERTKRLDAEGRPVIEYGFLPAGVLEDIQDYWYWLSTNAYTWWGMDR
                     AAQALSDIHHPESDRLLREATLFKNDLVRGFTASLQRSAVVRLQDGTAVPHFPSHQHL
                     RGRSYGWIRETLEGAIHLLRCKLIPPESRMGTWVMKDYEDNLYLSEQFGYSGASLPDR
                     ERYWFSRGGFSQQPNLLCSPLPYLFRNEIKHYLRAYFNAFAAGFHADTRMITEHPLPH
                     LGDWAGDHFKTSDEAQSAYWLRLMFVYEEAETLYFGYALPRYWLRPGNRISIQRARTY
                     FGETSVSYEVSDSGRQIVARIAPPTSTPPSEFRIRFRHAEGKPIRSVQVNGASWEDFD
                     HEFVRVPATASPLEVVASFGGRGKR"
     gene            complement(4403..4876)
                     /locus_tag="PLT86_09665"
     CDS             complement(4403..4876)
                     /locus_tag="PLT86_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT36918.1"
                     /translation="MDTVWGFLSSLGEPAARLTLAVLASSVIGWEREIGGHPAGLRTN
                     IMVALGAAIFTLLSQDIVAKGTGDPTRVIAGVAQGIGFLGAGTIFLDANRIKGLTSAA
                     TIWVMGGLGIAWGAGSYPTAILGTLAAFVVLRTIKRLELVTAEKLRNRVDRRDTL"
     gene            complement(4984..5892)
                     /gene="ribF"
                     /locus_tag="PLT86_09670"
     CDS             complement(4984..5892)
                     /gene="ribF"
                     /locus_tag="PLT86_09670"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOT36919.1"
                     /translation="MASTAITVGSYDGVHSGHRAMIAQLCASARERDLQSILVTFEPH
                     HRIYFHRETKPFLLTTTSEKRVLLQETCLDGVIVLPFSAELAALSARDFIKGILVERL
                     GARLLLVGHDQAFGKDQCTGRDAITACAAPLGVEVLSPAPVSSDGRVVSSTRIRGLIR
                     TGSIAPANHLLGAPYLVSGRVVHGRERGRSLGYPTANLSIEDPFKLIPGEGIYAVFAS
                     CGGSKFAGMGYIGTRSTFGETDPVVEIALLDTNVDLYGQELQVRFVERVRADHRFPNA
                     DALLAQIRRDEQAIRSILAAHRDSVA"
     gene            6176..7144
                     /locus_tag="PLT86_09675"
     CDS             6176..7144
                     /locus_tag="PLT86_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT36920.1"
                     /translation="MSASRNSERGKTIVVIGFPARDTKHMPDGSKVDSLGGVLFTAIP
                     LAVLSRRRGHRVVPVSSFGVDCAAEGEDALRAAGCELEHVRVVQAHTQHSFITFTSDN
                     ERWERVTGRLPALGKEDLHCVREADFVVVNFITGAEMEYDTFRWLAEEYQGPIIMDYH
                     TLALGTNPDGRREPRRRTDWAAWFQHGTVVQMNEAEAGALAGHSLEDARACAAFCRDL
                     LSLGPDAVVITRAEKGAVGAERVVRHGREAWATYEVPAPNVPHVVDTVGCGDVFLAGL
                     AVGWIQWHALETAMRFAAGVAALHVEYEGLTGAERLESVWEAFEGP"
     gene            7144..8145
                     /locus_tag="PLT86_09680"
     CDS             7144..8145
                     /locus_tag="PLT86_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOT36921.1"
                     /translation="MVRWWRTNMDRTGLGAGQIAGLFLCAMIFIPEGAPASLPAQRRA
                     AGNWEVTRFGNDLVIEHKPHMRSSAERAAAIVQMARRRFYALADTSFPFEIRVLLASS
                     ATEFARLTRGIVPDWGAAAAVPDEGLMIISGLSEEKPMSEAIYHEVAHVALHALSRGP
                     VPRWFDEGIAMRLSDEWSIAYSVRLARSALANRLLPLHSVERVLSFEREQAALAYAVS
                     FAAVRWFEKRHGDRALALLLRSLASHSFDEAFVRATGSDSESFEREWLRSARNSYALV
                     GLADDMWIWSILIPGLFFLALGVRWWRNRRTLARWKREDRDSSGSDEPLDEHVSETY"
     gene            8120..>8815
                     /locus_tag="PLT86_09685"
     CDS             8120..>8815
                     /locus_tag="PLT86_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36922.1"
                     /translation="MSMSAKHIESLVARYLVTRIKTAPPEDVGKWAKRISNTGWHLFS
                     RQRRVACANVERAFPELDEKAAIGMARASFRHAAVALVDAVRMPERLPDFVWTCPDVL
                     GPILEKKLPAIVISGHIGNWEVAGAAIATRGGRLHLIVATPTSPGVAGLVHRLRTDWG
                     VIPHAREKSLLPVARALRRGELVGTAVDQWPARRSVRVTFLNQPTPFASGLFDYAIRT
                     GVPVVAIWAIRTNQ"
BASE COUNT         1779 a         2604 c         2591 g         1841 t
ORIGIN      
        1 tgggagatga tgcacgtcaa tcgttgcgcc ttggctacgt atcatcgcgt actccagcgc
       61 ggcctgtaac tcccggacgt tgcccggcca tcggtaggaa cgaaagagcc ggagtacgtc
      121 gtcactgata ccgggcacag cagttccgtg tctgtcagcg attcttgaaa ggagcctgtg
      181 tacgagcagg ggtatatcat caaccctttc cctgagcgac ggtacgtgga tcggaaagac
      241 gttgagccgg taaaagagat cttcccggaa tcggccttcc gagaccatgt cgctcaggtt
      301 cttgttcgtt gctgaaatca gtcgtgcatc cgtccgacgt ggcttgttct cgcccacccg
      361 ctcgaacacg cgctcctgga ggactctgag cagcttcacc tgtatcatcg ggggaatttc
      421 tgcaacctcg tcgaggaaca gcgtgccgcc gtctgctgct tcgaatcttc ccagacggtc
      481 gctcaacgcg ccggtgaacg cgcctttgac atggccgaac agctcgcttt ccagcaaccc
      541 ttctggcaat gccgcgcagt tgacagttac cagcggcttg tccgcgcgat tgctcagttg
      601 atggatagcc ctcgccacga gctctttacc ggtacccgtc tcgccctgaa tgagcacggt
      661 ggcgtcggta cgggctacct cacggacaag ctggaacacc tgctgcatcg gttcgcttgc
      721 gccgacaatg ttttcaagac cccgcgcgcc ctcgagattt gccctgagac gcgagatctc
      781 cgtttcgtcg cgcaataaga ctactgcacc gagaagtccc gtctcgtcgg aaagcagttg
      841 cacgcgtgcg attaccgaga agaaaccgtt ctctccagcc ctgagttgca atcgttccgg
      901 cgaagatggc ttcccggaac cgattgctcg ctgcaacagc cggacctctc tcccgcagcg
      961 ttccgaaaga atctcgtcaa tgttctcacc ggtgtgcgat ctcaaattgc cgccaagcag
     1021 cctgaccgcg gcggtattcg tgtgaaaaat ctggccatcc ttcgtaactg ccacgatgcc
     1081 gtcttcgaga gcctcgaaaa cctgttccag gttccggcgc tttgccatgc acgtttcgag
     1141 ggcattaccg atcggcaaca cgttgttcgg ggtctgcaaa ccagatctcc aacgatgagt
     1201 tctgtgttca acgcttcccg cggccgccaa acgacgcaac aacctccaga ggggacgccg
     1261 tggcgggcac acgcacaaac tcgtggtcaa aatcctccca cgacgcgcca ttcacctgaa
     1321 cagaacgtat gggcttccct tcagcgtggc ggaaacggat gcggaattcc gacggcggcg
     1381 tgctcgttgg aggcgctatt cttgccacga tttgcctccc actgtcgctg acttcgtatg
     1441 atacactcgt ttctccgaag taggtacggg cgcgttggat cgaaatgcga ttaccgggtc
     1501 tgagccagta acgcggcagc gcgtagccga aatacagggt ttccgcctct tcgtaaacaa
     1561 acatgagacg aagccagtac gcgctttgag cctcgtcaga ggttttgaaa tggtcgcctg
     1621 cccaatcacc gagatggggc aacggatgct cggtaatcat tctcgtgtcg gcgtggaatc
     1681 ccgccgcgaa tgcattgaag tacgccctca ggtaatgttt gatctcattt cgaaagagat
     1741 agggcagcgg gctgcacagc agattgggct gctggctgaa tccaccgcgg ctgaaccagt
     1801 accgttcacg atcaggcagg cttgcgccgc tgtaaccaaa ctgctccgaa aggtaaagat
     1861 tgtcctcgta atccttcatc acccacgtgc ccatccgaga ctcgggcgga atcagtttgc
     1921 agcgcagcag atggattgcg ccctcgagag tctccctgat ccagccgtac gagcgaccac
     1981 gcagatgctg atgcgaaggg aaatggggaa ccgcagttcc atcctgcaac cggactaccg
     2041 cggatcgctg cagtgaagcg gtgaaacctc tgacgaggtc atttttgaaa agcgttgctt
     2101 cgcgaagaag tcgatcactc tcgggatggt gtatgtcgct cagggcctgt gccgccctgt
     2161 ccatgcccca ccacgtgtag gcgttggtgg ataaccagta ccagtaatcc tggatgtcct
     2221 caagaacgcc tgcaggcaga aaaccgtact caatgacggg tctgccttcg gcatccaacc
     2281 gctttgttcg ttcgcgctgt tcggtaatcc agtcgcatgc ttccactatc ttgcccgcca
     2341 cacggtggag ccagtcgcga tctcgtgtca ggaagtagtg ctccgcaagt ccccaaagag
     2401 cccatccatg attctggttg tagttcccgt gttcgctgcc tcccgccccg tacaacacgc
     2461 cgcgatgcga cgtgaaatcg cccggaagag gaacggttcc ctgaaaatgg aggaatagct
     2521 cgtacccatt cgcagcttcc tgagagtaac cgcgtcggtc aagatcagat agctgcatga
     2581 ccgactcgtt cgtgtacgcc ccgtaccggg aggagccgac acgcgcaata tcgagttcgt
     2641 gtcccccgga aacgggcgac gcggggtgac gctcgtgatt gatcagaagg tgtgtgatgt
     2701 gtgcccggta gaagtcattg ataaggggct caggcgttac aatccgggtt ccagcagcga
     2761 tcctccgttc ccagtagtcg gtaacctcct gcaacgcgct ctgcggctgg atcacctcca
     2821 acaaaccgag ttccgcctca ttcagtccga aaaaggggag cttgaacgcg atggtagtgc
     2881 attccgaagg ccgcagacgc aacgaatagt ggaccccttc cgtggttctg gaaagcctgc
     2941 ctttccggtg cgtgatcaca caccggagtt tcccgtgttg attcctcaca tagccgtggc
     3001 gtgcaagctg cagtcgttcc tgcgcgtcgc ccgctgcgac ccggagagtg agggttccgt
     3061 ccttttccac gttcgaggtg ttggtagcct tcacgaggac gatcgcaaca accgggtcat
     3121 ctccgcgtat cggtgtgtcg tccagtgaac gatcgaggag agtggcgaac gccgtctgtt
     3181 cgtaccgcag cccatcagtt tcccatgaag tcgtcatcac cgggaggtaa ccgcgtaacg
     3241 gcacgcgctg tgtaggcggt tctgcaggga gaccaaagga ccacctcacg gcgttcggtt
     3301 cctctttgac ccgctccgtt tcacgccccg gcattttctc aaaccactgc ctctcgtgcg
     3361 aacggaacac atctccattc ggctccacgc cgaaacgctg cctgcgcccc tcaacaccca
     3421 acacgaagta gaagggtctt ttcgccggca tatcctgcca cgcgcgcgca agtgtctgtt
     3481 cgggcatctc gaacacgcgg tcgtaatatg tcctgctttt tggccgcgta cgccggaatt
     3541 gccgcaacgt cgctttgtgc cgcgtttcag acaccagaac gccaaaatcc tcgagatgaa
     3601 tcggcccctg gtcaagatcg ttggccgcaa acgaaaatgt tgcttcgccc gtgcggacgg
     3661 tcactaccgt gtggtcgcgg gtcgtggggt cttcgtttct ggtggtccat acaccaaatc
     3721 ggaccagttc agaaccagct ttgtgttgtc tcagaagttt ggcacagccc tcgccggctg
     3781 cgtcagcaag tttgggcgaa ggcttctggc tggcgaggtg tccgttacaa acctcgacaa
     3841 gccatgtgct ctctttttcc gctgattgcc cgcgcgtttc acgttctacg acgatgtccc
     3901 tgcgaaacca cgttgaatcg gtgtacgcct cccaggaaat gatcgcaggt agaattttgt
     3961 cggacacgag acgaatacgc agtgtgtgcc gaaatcgagc cgcaaaatcc ctcatttccg
     4021 caaattcgcg cgtattcagc gggttgaacg tgaaaatcca ttgcttgtcg gcagcggcga
     4081 gaaccgtatc ggcaacctgc caccggccgt tgaaccagtc gtcctcggcc atccatccgg
     4141 ggaatccgcc tccggtcacc tgccccttcg gcacgcgttt ctccggccat tggcccttcc
     4201 agtactccag tcggacgtcc gttgcggcgg gaacatcatc ccgccagtgc acgacaacgc
     4261 gttcgatgtc ccgcgcttcg caccattgaa gcccgctgtc gaatcttggc gtccacgcca
     4321 tccgttcggc gaacggggcc atatcggtcg gggtaccctc ggcgaaaaag tgcacgggat
     4381 tgtcctcctt gcgcgatatc tgctacagtg tgtcccttcg gtccacccgg ttcctaagct
     4441 tttccgcggt taccagttcc agccgtttga tggttctcag cactacgaac gcggccagtg
     4501 ttccgagaat cgcggtagga taagaccctg ctccccatgc gatgccgagt ccacccatca
     4561 cccagatggt ggctgctgag gtgagaccct tgattcggtt cgcatcgaga aaaatcgttc
     4621 ccgcaccaag gaaaccgatt ccctgcgcta ctccggcgat cactcgtgtg ggatcacccg
     4681 tccctttcgc gacaatatcc tgcgatagga gggtgaagat ggctgcgcca agggcaacca
     4741 tgatgttcgt tctcaatccc gccggatgcc cgccgatttc gcgttcccat cctatgacac
     4801 tgctcgcgag caccgcgagt gtcaatcgcg ccgcgggctc acccagcgat gataagaaac
     4861 cccagacagt atccacgacg tcaaaccttc catggattta acgtttgact cctcaaagag
     4921 tcaaatggaa gttcccaaaa tacgagtatg gacagcagcg ccagtccaca gcgcacattg
     4981 agttcacgct accgagtcgc ggtgtgccgc gagaatgctc cggatcgctt gctcgtcgcg
     5041 cctgatctga gccagaagtg catccgcgtt cgggaaacgg tgatccgccc ggacgcgttc
     5101 cacgaaacga acctgcaact cctgcccgta aagatcaaca ttcgtgtcca gaagcgctat
     5161 ctcgacgacg ggatccgttt ccccgaacgt cgaccgcgta cctatgtacc ccattcctgc
     5221 gaatttcgag ccgccgcagg acgcgaaaac ggcgtagatc ccctcgccgg gaatcaactt
     5281 gaatggatcc tcgatcgaaa gattggccgt gggatagccc agggaacggc cgcgttcccg
     5341 tccgtggacc acgcgcccgg agaccagata cggcgcgccg agaaggtggt tcgcgggagc
     5401 gatgctcccg gtgcgtatca agcctctgat tcgagtgctg gataccacgc gcccatctga
     5461 cgaaacggga gcaggcgaaa gaacctccac gccaagagga gctgcgcatg ccgtgattgc
     5521 gtcccgaccg gtgcactggt ctttcccgaa ggcttggtca tgccccacga gcaacagccg
     5581 tgcgccaagt ctttccacaa ggatgccttt gatgaaatct cgcgccgaaa gtgccgcaag
     5641 ctcggccgaa aaggggagga caatcacgcc gtccaggcac gtttcctgca aaaggacgcg
     5701 cttttcggag gttgtcgtca gaagaaaagg cttcgtttcc cggtggaagt aaatccggtg
     5761 gtgcggttcg aacgttacca gaatcgattg aaggtcgcgc tcgcgggcag atgcgcacaa
     5821 ctgcgcgatc atagcccgat gaccagagtg tacaccgtcg taggacccga cggtgatcgc
     5881 cgtgctggcc atatggggaa gatccacgga ggactgaatg caagaaaaac gcatgacaca
     5941 aaaatactcc aagcagccgc ggcgggacaa tgatccggct cgccggctgc gcgccagacg
     6001 acggtcggcg ttaccttatc attcagccgc tctccgcttt ggcggaaccg aggcgcgtgg
     6061 aatccggtgc gttcccgatg cagtcggccc gaaaagtagg tctgccagtt tgcgcagatc
     6121 atttgccaaa aaccgtgcga gtgtcgtggt gtgtggcact accgaggtag aacgaatgtc
     6181 tgcatcccgc aacagcgagc gcgggaaaac aatcgttgtg atcggttttc ctgcgcgcga
     6241 cacgaaacac atgccggacg ggagcaaagt ggattcgctc ggcggggtgc ttttcactgc
     6301 gattcccttg gcggttctct ctcggagacg cggccatcgc gttgttcccg tgtccagctt
     6361 cggggtcgac tgcgcagcag aaggagaaga tgcgctccga gcagccggtt gcgaactgga
     6421 gcatgtccgt gtggtgcagg cgcacaccca gcactccttc attacgttca ccagtgataa
     6481 tgagcgctgg gaacgggtaa caggaagact cccggcgctg ggaaaagaag acctccattg
     6541 tgttcgcgaa gcggactttg tggtcgtgaa tttcatcacc ggggcggaaa tggagtacga
     6601 cacattccgt tggctcgccg aggaatacca gggcccgatc atcatggatt accacacact
     6661 tgcgctgggt accaatccgg atggccgcag ggagcctcgc agacggaccg actgggccgc
     6721 gtggtttcag cacggaacgg tagttcagat gaatgaggct gaagcgggag cacttgccgg
     6781 gcactccctg gaagacgcca gagcgtgtgc agcattctgc cgcgaccttc tgtccttggg
     6841 gccggacgcg gtagtgatta ctcgtgcgga aaagggtgcg gtgggcgctg aacgcgtggt
     6901 gagacacggg cgagaggctt gggcgacgta cgaagtaccc gcgccgaatg tcccacacgt
     6961 agtcgacacc gtcgggtgtg gtgatgtctt tcttgccgga ctggcggtgg gatggattca
     7021 gtggcacgcg ctggaaacgg cgatgaggtt cgcggcaggc gtggccgcac ttcacgtcga
     7081 gtacgaaggg ttgaccggag cggaacgact ggaatccgtc tgggaagcct ttgaggggcc
     7141 ctgatggttc gatggtggcg gacaaacatg gaccgaaccg gcctgggagc cggccagatt
     7201 gccggcctct tcctgtgcgc aatgatcttc atcccagagg gcgcacccgc ttcacttccg
     7261 gcccagaggc gtgcggcggg aaactgggag gtcacccggt tcggaaacga cctcgtcatt
     7321 gagcataaac ctcacatgcg ctcatctgca gagcgtgcgg cggcaatcgt ccagatggcg
     7381 agaagacgtt tctacgcact tgcggacact tctttcccgt ttgaaatccg cgtgcttctc
     7441 gcatcctccg ccacggaatt tgccaggctg acgcgcggga tcgttcccga ctggggtgct
     7501 gccgcggcgg ttccggacga agggctgatg atcatttccg gtctgtctga agaaaaaccc
     7561 atgtcggaag cgatctatca tgaagtggcg catgttgcgt tgcacgctct ttcccggggc
     7621 cccgttccgc ggtggttcga cgaagggatc gccatgcggc tctccgacga atggagcatc
     7681 gcatacagcg tccgtctggc aaggtcggct ctggcgaacc gattgttgcc gttgcattcc
     7741 gtagaacgtg tgctttcctt cgaacgggag caggccgcgc ttgcctacgc cgtaagtttc
     7801 gcggcggttc ggtggtttga gaaacgccac ggcgacaggg cgctggcgtt gctgcttcgt
     7861 tctctggcct cgcactcttt tgatgaggcc tttgtaagag ccacgggttc agacagcgag
     7921 tcgtttgaac gcgaatggtt acggtccgcg cggaattcgt acgcacttgt cggattggcg
     7981 gatgacatgt ggatttggtc gattcttatc cctgggctct tcttcctggc ccttggagtt
     8041 cgatggtggc gcaatcggcg aacgctggcg cgctggaaaa gggaagaccg ggatagttcg
     8101 ggttccgacg agcctctcga tgagcatgtc agcgaaacat attgaaagcc ttgttgcgag
     8161 gtatctcgtg acgcggatca aaacggcgcc gccggaagac gtcgggaaat gggcgaaaag
     8221 gatatcgaat accggatggc acctgttctc gcggcaacgt cgagtggcat gtgccaatgt
     8281 ggagcgggca tttccggagc tcgacgaaaa agccgccatt ggaatggccc gtgcgtcgtt
     8341 tcggcacgcc gcggtggcct tggttgacgc ggtacggatg ccggaacggc tgccggattt
     8401 tgtctggact tgtcctgacg ttctgggacc catcctcgaa aagaaacttc ccgcgattgt
     8461 gatttccggc catatcggga actgggaggt ggccggggca gcgatcgcaa caaggggagg
     8521 caggctccac cttatcgtcg cgacaccgac cagccccggt gttgccggcc tggtgcatcg
     8581 tctccggaca gactggggcg tcataccgca cgcgcgggaa aagtcgcttc ttccggtcgc
     8641 tcgtgccctc aggcgaggtg aactcgtcgg aactgcggtc gatcaatggc ccgcccggcg
     8701 ttcggttcgc gtaacattcc tgaaccaacc aaccccgttt gcgtccgggt tgttcgatta
     8761 cgccattcgc acgggggtgc cggtggtcgc catttgggcg atacggacga atcag
//
LOCUS       DAOSOO010000131         8775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7972_length_8775_cov_4.351147, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000131 DAOSOO010000000
VERSION     DAOSOO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7972_length_8775_cov_4.351147"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLT86_09690"
     CDS             <1..249
                     /locus_tag="PLT86_09690"
                     /inference="COORDINATES: protein motif:HMM:NF018829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HOT36923.1"
                     /translation="MWNLEQLRSLGIEPTRCRTIVVKAAIAHRAAYSPIAGRMVELDT
                     PGITALDIRRFTYRHQRRPVFPLDDDFHPLIQDQTTGA"
     gene            252..1088
                     /locus_tag="PLT86_09695"
     CDS             252..1088
                     /locus_tag="PLT86_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT36924.1"
                     /translation="MRLGAPVFDEAGTPEAWAAAHGIAGYRAAYCPVDGTASDDVVNA
                     YAEAAKKFDIVIAETGAWSNTISPDTETRDAAIRKCQDQLALADRIGARCCVNIAGSR
                     GTQWDGPHPDNLTRHTFDLIVQTVREIIDAVKPVRSYYTLETMPWVFPDSPESYSELI
                     RAVDRTRFGVHLDPTNLVCSPQRYARTGDLIRECFEMLGPHIRSCHAKDIVLSEKLTV
                     HLDETRPGLGNLDYRVFLRELSRLDPDTPLMIEHLPTRQEYEAAALHIRTVAREIGVE
                     GL"
     gene            1085..2056
                     /locus_tag="PLT86_09700"
     CDS             1085..2056
                     /locus_tag="PLT86_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HOT36925.1"
                     /translation="MKTAQVVFTGCCRAELVESPARTEALEAHEVIGTTLATIISAGT
                     ELAVYQGLAGGTYPAHPGYAAVFRIDAVGSEVKGFRPGDVAFCMGPHRSFQRTKEEFV
                     VRVPSEVPPEQAVFARMMAVSMSTLTTTAARPPAAVLVTGLGLVGHLAAQIFASCGYD
                     VTAVDPLEVRRTIASDSGIARVFASVPPEIAGSVSLAVECSGNERAVLDACGVVRKGG
                     EVVLVGVPWKKRTDLAAFDLLHAVFHRYVVLRSGWEWEVPLLPTDFRQNSIYGNIAAA
                     MRWISEGRVRVEHLFRVESPRRATEAFSALASGSCDRLGIVFDWTNL"
     gene            2263..2667
                     /locus_tag="PLT86_09705"
     CDS             2263..2667
                     /locus_tag="PLT86_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOT36926.1"
                     /translation="MANLVEVEGIGEAYAKKLAAAGIKTTDALLAKGATPEGRKEIAA
                     KSGISAKMILEWVNHVDLFRIKGVGQEYADLLEAAGVDTVPELAQRKPGNLVEKMAEV
                     NKKKKLVRQLPVETQVARWIAQAKELPRKIKY"
     gene            2843..4120
                     /locus_tag="PLT86_09710"
     CDS             2843..4120
                     /locus_tag="PLT86_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT36927.1"
                     /translation="MGNCYDVIVAGGGTAGTAAAIAASRRNHKVLLIEEQNCLGGTST
                     SGGVGEWFASLQGMGNIFDAVVAGLEKFGARFAKPRHFNPEVLKSAWQLLAEEAGVQI
                     LFHASVCGAERSGRAVSRIEVASCSRLFSAEAKYFIDATGEGDLSALAGAEYMQGEPG
                     TRKVLHMSLVAWMYDTGTLQTPYLPASLEPVRSREELPGLGAGVLVDRNRVYLNATKV
                     LDRDPTDPWSLSDAEREARHQLLRVVHYLQTHEYPTFALASSGARIGIREGRRVVGDC
                     VLSRADILDRREPLDFEDGVVVATSQIDFHSLKKAGNSGWRQPVLPYSIPLRCMLAKG
                     FPNLAVAGKCISVDQVVHSSCRMTPTCCAMGQAAGTAAALAIETGVADLRDVPISSLR
                     QTLNADGMELNPARHTAFAPHDTRLDADDHVGI"
     gene            complement(4105..6957)
                     /locus_tag="PLT86_09715"
     CDS             complement(4105..6957)
                     /locus_tag="PLT86_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT36928.1"
                     /translation="MVYSLDGEWLIACDPRNEGRGQDWWAAVRPEACLTSVPWIIQGV
                     FPGYHGVAWYWRQFSVHRNPYPSGRYLLRFWAVDYLAEVWVNGQRVGAHEGAETPFVL
                     DITEAVVPDGKNLVAVRVLNPTNEPIDGITLNETPHRNKTVPIFAGASFNHGGIMDSV
                     ELIVAPAVRVADLVAHPDPVTGIVNVRVLLVNALAKAASCALRVSIAPSPSGETLETF
                     RGERMVDAGETWVSVQLKIDRPHLWQLNDPFLYHVTAEVSCATCSAASEYSVRCGFRD
                     FRFENGYFRLNGTRVFLRGSHTGNHAPIGQQLPPDPDMFRRDLLYVKSMGFNMIRFIA
                     GVATRYQLNLCDEIGLLVYEESYAGWLLGDSPRMKRLFEQSVCEMIRRDINHPSIVIW
                     GLLNETNDGPVFQHARNSLPAIRAIDPDRVILLNSGRWDGQQSIGSLANPGSTEWECC
                     LGGEAPGAAVTASTWGGYFERAGDAHAYPRVPHTAETISFLRRLGQDTGPVILTEYGI
                     GSAVDLWRVVRLFEQWRAEKCEDAVFYRDLLEQFLTDWRRWRLEEMFPRPSDYFAACV
                     RKMAGQRLLGLNAIRSNPHIIGHSMTGTVDQGLTGEGLFTTFRELKPGTADAVADGLA
                     PLRWCLFAEPVHAYEGAPVRLEAVLADEDVLHAGAYPACFKVVDPSGTTVFESHSSYR
                     VHDNGSERPLAAPVFADEVRISGGPGAYRFLAEFESGAAAAGGEAVFYVSRKPDTPLR
                     GVTVAVWGDDSDLENWLAAHGAEVVVPDRDTADQRCPIVLSKTPVHQGAPGGFDDLTR
                     RLQRGSTVICLDPRVFADGDDQTAFLPLDRKGRFHSIVGWLYLKDEWAKRHPIFDGMP
                     AGGLLDYDFYREIIPDLLYSPGEESYEAVAGAIRASQGYSSGLMISVHRVGAGRLIIN
                     TFRIRENIGTHPAADMLLMNMLRVASRSDGDNHMPT"
     gene            7116..>8775
                     /locus_tag="PLT86_09720"
     CDS             7116..>8775
                     /locus_tag="PLT86_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36929.1"
                     /translation="MNTAWRTCGRHGEMTGACLAGTVRNRLFLDFPAHPRQIRQTTLA
                     LAYGRNTANGTPSPPTMPPGVTHDASSVPFPVIRWPRVLIWGLSVVVLMNVWMAYCEY
                     VVRASRLNGSHFPVALVAAMAALVLAVWPLLKAIGGSNWGINTPERHLILAMGFVGAA
                     IPANGLTGFMMGIIATPYYYATPENNWASFHPFIPGWIVPPDKHHAMRWFFNGLPPGE
                     PIPWRVWWVPLIWWFSLAAAIMGVCLALSIILRKQWVEYERLSYPLVEASKCLLAGND
                     DPRGRRPVWQSRLFWVGTTIAAFILVYNTFAWFDPRWPMIDIAGRGIVLFRGVPDFKT
                     RINFLTLGLSYFAPQQVLGSLIVGCLVTTAEAGAFARVGFSLDPAGDSWTNDNVAIGW
                     QSFGAMMVFVLWGLWQARRHFVAVWRSAWAGTDDGTEMLSYRAALIVLTLSLFYMTMW
                     LHEAGMDVSFAITFVFFTVIGYLALGRMVAEAGYVYQRVPISPQSATVYTLGVESFHP
                     ATMAGFAFSYALIANGRGLFMPAIVLATRLADSSGKTARKALGAI"
BASE COUNT         1804 a         2534 c         2677 g         1760 t
ORIGIN      
        1 atgtggaatc tggagcagct tcgttctctg ggcatcgagc caacccggtg ccgtacaata
       61 gtcgtgaaag ccgcgatcgc ccatcgggct gcctattcgc ccatcgccgg acgtatggtg
      121 gaactcgata cacccggaat caccgcgctg gatatccggc gtttcacgta ccgccaccaa
      181 cgaaggccgg tgtttccgct ggacgacgac tttcaccctt tgatccagga ccagacaaca
      241 ggagcctgag aatgcgactt ggtgccccgg ttttcgacga agcaggaacg ccagaagcat
      301 gggctgcagc acacggcata gcggggtacc gcgcggcgta ctgcccggtg gacggcacgg
      361 cttcggatga cgtcgtcaac gcgtacgcgg aagcggcaaa gaaattcgac attgtgatcg
      421 ctgaaacggg tgcgtggtcc aacacaataa gccctgatac ggagacgcga gatgccgcaa
      481 tccgaaagtg ccaggatcag ctggcactgg cagaccgaat cggcgcgcgc tgctgcgtga
      541 acatcgccgg atcacgggga acgcagtggg atgggcctca tcccgacaac ctcacgagac
      601 acacgttcga cctcattgtg cagaccgtca gagagatcat cgacgcagtg aaacccgtgc
      661 gtagttacta cacgctcgaa accatgccgt gggtgttccc cgactccccc gagtcgtatt
      721 ccgaactcat ccgcgcggtg gatcggacaa ggttcggtgt gcatctcgac ccgaccaacc
      781 tcgtgtgcag cccacagcgg tacgcacgca ccggtgacct gattcgtgaa tgcttcgaaa
      841 tgcttgggcc gcatatcaga agctgccacg cgaaggatat cgtcctgagc gagaaactca
      901 cggtccatct ggatgaaacg aggccgggac tgggaaatct ggattaccgg gtatttctcc
      961 gtgagctttc caggctcgat cctgacacac cacttatgat cgaacacctg ccgacgcgac
     1021 aggaatacga agctgccgca cttcacatca ggacggtcgc gcgggaaatc ggagtggaag
     1081 ggctatgaaa accgcgcaag tcgtgtttac cgggtgctgt cgggcggaac tggtggagtc
     1141 tcccgccaga acagaagctc tggaggcgca cgaggtcatt ggaacaacgc ttgccaccat
     1201 catcagcgcc ggaacagagc ttgcggtata tcaggggctc gcgggaggga cctatccggc
     1261 tcaccctggg tacgccgcgg tgttccgcat cgacgctgtc ggttctgagg tgaaaggttt
     1321 tcgtccgggc gacgttgcct tctgtatggg gccacaccgt tcatttcagc gaaccaaaga
     1381 ggaattcgtc gtacgtgtgc catctgaggt gccccctgaa caggctgtgt tcgcccggat
     1441 gatggcagtg tcaatgagca cgttgacgac aactgccgca cgaccgcccg cagcggttct
     1501 ggtgacgggt cttggattag tcggtcacct tgccgcgcag attttcgctt cctgcggata
     1561 cgatgtaacg gcggtggacc cgctggaagt gcgtcgaacg attgcctctg actcaggaat
     1621 tgcccgcgtg ttcgcgagtg ttccacccga aattgcaggt tcggtcagtc tggcggtgga
     1681 gtgctcgggc aacgaacgcg cggttcttga cgcctgcggt gtggttcgca agggcggtga
     1741 ggtggtgctg gtcggcgtgc cttggaaaaa gcggactgat ctcgccgcgt ttgaccttct
     1801 tcatgcggtg ttccaccggt acgtcgtctt gcggagcggt tgggagtggg aggttccgct
     1861 cctgcccacc gatttcaggc aaaacagcat ctacggaaac atcgctgccg ccatgcgctg
     1921 gatttccgaa ggaagggtcc gcgtggaaca cctctttcgt gtggagtctc cccggcgggc
     1981 aactgaagcg ttctccgcgc tggcatccgg gtcgtgcgac cgtctgggaa tcgtatttga
     2041 ctggaccaac ttgtgaacga acgcaggggg cgcagtcaac cgcgcgaaca cgcctgcaca
     2101 tctccgaaga acgtagttga ctttgtccag aagcgctcgt acactactgc gacggaaggc
     2161 gtttgcttcc gtccgggagc aaatttcacc tcgcgtcgtg ttccacggaa cctgtgtttg
     2221 tgtggaacga gacaattccg cggctgagga aaggagtcag aaatggcgaa cctggtcgag
     2281 gttgaaggaa ttggtgaagc ctacgccaag aagcttgcgg cggcaggaat caaaaccaca
     2341 gacgcgttgt tggcgaaggg tgcgacgccg gaggggcgca aggagattgc ggcgaaatcc
     2401 ggaatcagcg cgaaaatgat cctggaatgg gtgaaccacg tggacctttt caggatcaag
     2461 ggcgtcgggc aggagtacgc ggacctgctg gaggccgcgg gggttgatac ggttccggaa
     2521 ctcgcacaac gcaaaccggg aaaccttgtc gaaaagatgg cggaggtcaa caaaaagaaa
     2581 aagcttgttc gacaactccc ggtcgaaaca caggtggctc gttggatagc tcaagccaaa
     2641 gaattaccga gaaagatcaa gtactgacct gcgaccgccc gtgtgcacct gcgggtggtc
     2701 ggaaggtgct ttctgctggc ggaatgtcga aaggtcagcc gcggagggtc tgtcgagcct
     2761 ttttggggct tgacgggccc tttctgtctt gatcggcgtt cgagctggag ttccacgggt
     2821 gcattgtgat ggtagggaga acatggggaa ctgttacgac gtcatcgtcg ccgggggagg
     2881 gacggccggg accgcagctg caatagccgc cagccgtaga aaccacaaag tattgctgat
     2941 tgaagaacag aactgcctcg gcggaacgtc cacttcgggc ggagtcggcg aatggtttgc
     3001 ctcactgcaa gggatgggca acatattcga cgctgtcgtc gccggtctgg agaagttcgg
     3061 cgccaggttc gcgaaacccc gccatttcaa tcccgaagtg ctcaaaagtg cctggcagct
     3121 tctggcggaa gaggccgggg tacaaatcct cttccatgcg agcgtatgcg gtgcagagcg
     3181 ctctggacgt gcggtctcgc gcattgaggt tgccagttgt tctcgattgt tttcggcgga
     3241 ggcgaagtac tttatcgatg caacgggcga aggagatctg agcgcgctgg ccggcgcgga
     3301 gtacatgcag ggcgagccag gcacccgaaa agtgcttcac atgagcctgg tggcgtggat
     3361 gtacgatacc ggcaccctcc agacgcccta tctccccgca tcgctcgaac cagttcgaag
     3421 cagggaggaa ttgccggggt tgggcgcggg agtactggtg gaccggaaca gggtgtattt
     3481 gaacgccacg aaagtgcttg atcgtgaccc cacagatccc tggagcctga gcgacgcaga
     3541 acgggaagcg cggcaccagt tgcttcgtgt cgttcactac cttcagacgc atgaataccc
     3601 cacgtttgcg ctcgcatcca gcggggcgcg catcggtatc cgggagggcc gaagggtcgt
     3661 tggcgattgc gtcctttccc gggccgacat cctggacagg cgggaacctc tcgacttcga
     3721 ggacggcgtg gtggttgcaa caagtcagat cgactttcac agcctgaaga aagccggcaa
     3781 ctccgggtgg cgccagccgg ttctccccta tagcatccct ttgcggtgca tgcttgcaaa
     3841 gggatttcca aatctcgctg tggcgggaaa gtgtatcagt gtcgaccagg tagtacacag
     3901 cagttgcagg atgacaccga cctgttgcgc aatgggacag gccgctggca cagccgccgc
     3961 ccttgcgata gaaacaggag ttgcagacct gcgggatgtg ccgatttcga gtcttcgcca
     4021 gacgctgaac gcggatggca tggagctgaa cccggcacgc cacactgctt tcgcgccgca
     4081 cgatacccgc cttgatgccg atgatcacgt cggcatatga ttgtccccgt ccgagcggga
     4141 tgcaacgcgc aacatattca tgagcaacat gtccgccgcg ggatgagtgc cgatgttctc
     4201 cctgatgcgg aaggtgttga tgatcaacct tcccgcaccc acgcgatgaa cggatatcat
     4261 caaccctgag gaatacccct gcgatgcacg aattgctccc gccaccgcct cgtatgattc
     4321 ctcgccaggc gagtaaagaa ggtccggaat gatctcccgg tagaaatcgt aatccagaag
     4381 gccacccgcc ggcatcccgt cgaagatcgg gtgtcgtttt gcccattcat ctttgagata
     4441 cagccagccg acaattgaat ggaacctgcc tttcctgtcc agcggaagga acgccgtctg
     4501 gtcgtcgccg tcagcaaaca cgcgcggatc gaggcatatg actgtcgagc ctcgctgcag
     4561 acgacgggtg agatcgtcga atcctcccgg tgcgccctga tggaccgggg ttttggagag
     4621 gacaatcggg catcgttgat ccgcggtatc gcgatccgga acaacgacct ccgccccgtg
     4681 tgccgcaagc cagttttcca ggtctgagtc gtcaccccac accgcaaccg tcacgccgcg
     4741 caacggcgtg tcgggttttc gcgacacgta gaaaactgcc tctccacctg cggcggccgc
     4801 acctgattcg aactccgcaa gaaagcgata cgcacccggc cctccgctaa ttcttacttc
     4861 gtccgcgaac accggcgcgg caagcgggcg ctctgagcca ttgtcgtgga cgcggtacga
     4921 ggaatgtgac tcgaaaaccg tggtacccga cggatccacc accttgaagc atgccggata
     4981 tgcgccggca tgaagcacat cctcgtccgc aagcactgcc tccagacgaa caggcgcgcc
     5041 ctcgtatgcg tgaacgggtt ctgcgaagag gcaccaacgc agcggcgcaa ggccatcggc
     5101 tacggcgtca gccgttcccg gtttgagttc tctgaacgtg gtgaacagtc cttcgccagt
     5161 gagcccctga tccacggtac ctgtcatgct gtgcccgatg atgtgcgggt tggatcggat
     5221 tgcgttgagc cccagcagtc gttgtccggc cattttgcgc acacacgcgg cgaagtaatc
     5281 cgatgggcga gggaacatct cctcgagccg ccaacgcctc caatcggtga ggaactgctc
     5341 gagaagatcg cggtagaaca cagcatcttc gcatttttcg gcgcgccact gctcgaaaag
     5401 ccggacgacg cgccagaggt cgaccgcgct gccgatgccg tattccgtaa ggataacggg
     5461 ccctgtatcc tgcccgagtc gccgtaagaa ggaaatggtt tccgcagtgt gaggcacccg
     5521 cgggtacgca tgagcgtcgc ccgcacgctc gaaatagcca ccccaggtgg aagccgtgac
     5581 tgcggcgcca ggcgcctccc cgccgagaca gcattcccac tccgtggacc cggggttcgc
     5641 gagtgacccg attgactgct gcccatccca cctgccgctg ttcagaagga tgacacgatc
     5701 cgggtcaatc gcccggatgg caggtaagga atttctggcg tgctgaaaca ccgggccgtc
     5761 attggtctca ttcaggagtc cccagataac gatgctggga tggttgatgt cgcgccggat
     5821 catctcgcat acggattgct caaacagacg cttcatgcgc ggggaatccc cgagcaacca
     5881 tcccgcgtat gactcttcgt acacgagcaa ccctatctcg tcgcacaggt tcagctggta
     5941 acgggtagcc actcctgcga tgaaccggat catgttgaat cccatggact tgacatacag
     6001 caggtcccgc cggaacatgt cggggtccgg aggcagttgc tgccctatcg gtgcatggtt
     6061 gcctgtgtgc gagccgcgga ggaaaacccg tgtcccgttc aaacggaaat acccattctc
     6121 aaaacggaag tcacgaaatc cgcagcgaac ggaatactcg cttgcggcgg aacacgtcgc
     6181 gcaggagacc tccgccgtaa catgatagag gaaggggtcg ttcagttgcc agagatgcgg
     6241 acggtcaatc ttcagttgga cactcaccca tgtctcgccg gcatctacca tcctttcgcc
     6301 gcggaacgtc tccagagtct ctccggacgg ggatggagcg atactcacgc gaagggcgca
     6361 agaagcggcc tttgccaagg cattcaccag aagaacccgc acattgacga taccggtaac
     6421 ggggtctgga tgggcaacaa ggtccgcgac gcggactgca ggggcgacga tgagctccac
     6481 cgagtccatt atgccgccgt ggttgaatga tgcgccggca aagatgggaa cggttttgtt
     6541 ccgatgggga gtttcattca gggtaatccc gtcaattggc tcgttcgtcg ggttcagcac
     6601 acgcacggca acaaggttct tcccgtccgg aacgactgct tcagtgatat ccagtacgaa
     6661 cggggtttct gctccttcgt gagcaccaac cctttgaccg ttcacccaga cttcagcaag
     6721 atagtcgacc gcccagaacc ggagcagata tcttccggag gggtagggat ttcggtggac
     6781 cgagaactgc cgccagtacc atgcgacccc gtggtatccg gggaagacgc cctggatgat
     6841 ccagggaacg ctggtgaggc aagcctcggg ccgaactgcg gcccaccagt cttgaccccg
     6901 cccttcgtta cgggggtcac acgcgatcaa ccattcaccg tcaaggctgt ataccacggc
     6961 aagtcctttc ccgaaaggcg atcaggtgcc agatccatcc ggctgttaag aaccgagcaa
     7021 aacagcaggc ggaaacgtga agcccgtacc cgggcactgg aatgctgatt gtaccaggtg
     7081 ctacggaacg tgcgccgggg gcaaggggcg actgaatgaa taccgcatgg cgcacgtgtg
     7141 gtcggcacgg cgaaatgacc ggcgcctgcc ttgccggaac cgtgcgcaac cggttattct
     7201 tggatttccc ggcacatccg cgtcaaatcc gtcaaaccac tcttgccctc gcatacggga
     7261 gaaataccgc gaacggaacc ccttctccac cgaccatgcc accaggcgtg acccacgacg
     7321 cgtcttccgt gcctttcccg gtgattcgct ggccgcgagt tctgatctgg gggctttcgg
     7381 tcgtggtgtt gatgaatgtc tggatggcct attgcgaata cgttgttcgc gcgagccggc
     7441 tgaacggcag ccattttcca gtggcgctgg ttgctgcgat ggccgccctg gtgctggccg
     7501 tatggccgct gttgaaagcg atcggcggaa gcaactgggg cataaacact cctgaacgac
     7561 acctgattct tgccatgggc tttgtcggtg cggcgattcc ggccaacggg cttaccgggt
     7621 tcatgatggg catcatcgcc actccgtatt actacgcaac acctgaaaac aactgggcaa
     7681 gcttccatcc cttcatcccg ggttggatcg tccctcctga caaacaccac gcgatgcgat
     7741 ggtttttcaa cggccttccc cccggagaac cgatcccgtg gcgtgtgtgg tgggttccgc
     7801 tgatctggtg gttttcgctc gcggccgcca tcatgggggt atgcctcgcg ttgtccatca
     7861 ttcttcgcaa acagtgggtt gagtacgaac gtctttcgta cccgcttgtc gaagcaagta
     7921 agtgccttct tgcggggaac gatgaccctc gtggacgcag acctgtgtgg caatcgcggt
     7981 tgttctgggt cgggacgacg attgcggcgt tcattctggt ctacaacact tttgcatggt
     8041 ttgacccgcg ctggcccatg attgatatcg ccggccgcgg cattgtgttg tttcgcggag
     8101 tgccggactt caaaacacgt atcaatttcc ttacactggg tctttcctac ttcgcacccc
     8161 aacaggtgct cggcagcctg atcgtcggtt gcctcgtgac aaccgcggaa gcaggcgcgt
     8221 ttgcccgcgt gggtttcagc ctggaccccg ccggagacag ctggaccaac gataacgtcg
     8281 cgatcggatg gcagagtttc ggggcgatga tggtgttcgt cctttggggc ctctggcagg
     8341 cgcgaaggca ttttgtggcg gtttggcgct ccgcgtgggc gggtaccgac gatggaacgg
     8401 agatgttgag ttaccgggct gcccttatcg tgctcacgct ctcccttttc tacatgacca
     8461 tgtggcttca cgaagccggc atggacgtga gcttcgccat cacattcgtc tttttcacgg
     8521 tcatcggtta ccttgcgctc ggacgaatgg tcgccgaagc gggctacgtg tatcaacgag
     8581 tgcccataag tccccagtcc gcaacagtat acacgctggg ggtggaatca tttcacccgg
     8641 caaccatggc gggttttgcg ttctcgtatg cgcttattgc gaacggacga gggttgttca
     8701 tgccggcaat cgtgctggcc acacgtcttg ctgattcttc gggcaaaacc gctcggaagg
     8761 ctctgggagc aattg
//
LOCUS       DAOSOO010000132         8773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_7975_length_8773_cov_4.799839, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000132 DAOSOO010000000
VERSION     DAOSOO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_8773_cov_4.799839"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLT86_09725"
     CDS             <1..818
                     /locus_tag="PLT86_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT36930.1"
                     /translation="ELVAISAAGISVYWVALPLLRLGLLLSLAALLLTELVVPGANDE
                     RSRILQLAPQGILSGHAFHVCRQDRSGLILYAERYNIAAKRAQRVAIVQLRGGVPVRR
                     IDASEMVWDDLGWQLQSALDRSFLGDSMVFASREAMRLPLLTIQPEDLARVDKLPEQM
                     SFLELRDYIRRTRLVGGDASRWLVDLHLKFAFPFANLMMVWIGFPLAARSWRGGRAAY
                     VGLTFLVGFLYFVVVRSGQALGRSGDLHPVVAAWGANALFFVIGAALFRWSRK"
     gene            complement(820..2730)
                     /locus_tag="PLT86_09730"
     CDS             complement(820..2730)
                     /locus_tag="PLT86_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36931.1"
                     /translation="MPEYDSVCRITYERYAVEIRPNDFTLALRDLALGAGWISGKPFI
                     RLRIPRPTGKPPVYWGFPEPYELVEWDSSCLSAEAEGRRLGVALEGLQTCYGRVGVEC
                     AIIIERDRVRFEVERVTGLPAGAEIIVDFPYRLGFVQAPGKEPVPGGGLILPRGAGIM
                     VDFSQREGAFPLENLIYSGGQNGYSMPLFGICDGVATLGTIVKTPFDCYLVGEINSGD
                     PPAYAVSPGFLFEAGRLDYSRRADYRAFEGGYGELASWYRSVLQAEKRYRTLEEKAEG
                     HPLVHTLPGSVLAEMALDFNGTGGGRTPEELADTAKRMGFPGLVSYATNIWQRPFFGT
                     NAVPPNRGTEEDLARGAQYARSVDKAYYLSLYENLIDMWPMTPGYDLAIMAKLRDGSP
                     RPNWYAEHLKMRSSTVCSVCRLETSRRDHPHLKELIGEGSIYIDVEGAMELNECFDSL
                     HPLTREQDAGMRRELLSHVRDLFGTVATESMPMDFLADIVDVGAYFPVYQFIGYGCSD
                     KPLLIPPAIPIPLFVMVYHGSVLNMTPKSTDFYTCDPLYVPLWGLMPDETDEFSLRIS
                     KTMRETSYAALVEHRFLAPPRVDTEKGFHSADVQFSRFSDGTCVLANFSGEPYSWMGK
                     EAQPGDYFLWRE"
     gene            2886..4160
                     /locus_tag="PLT86_09735"
     CDS             2886..4160
                     /locus_tag="PLT86_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT36932.1"
                     /translation="MPQMPEKTPGKTDWFIHDRFGMFIHWGLYALPARHEWVKSREMI
                     PDEKYDVYFRHFNPDLYDPTVWAKAARNAGMKYFVVTTKHHEGFCLWDSDLTDYKATN
                     TPYGKDLLKPMVEAFRAEGLKVGFYHSLIDWHHPEFPVDCTHPMRNDKEFRERAKDRD
                     ITKYAEYLHGQTRELLTRFGKIDIMWFDFSYPTQPDGGKGRNDWQSEKLYELVRKLMP
                     DVVLDDRLDLEKGYDIKTPEQWQPQEWVKVDGKPVVWEACQTLNGSWGYDRDNLDWKS
                     PALLLWMLVDSVSKGGNLLLNVGPTGRGEFDERALATLQAIGKWTRVNGRSIYGCTAS
                     DYTPPPDCRYTQNGNRLYLHVMAWPLRHLHLKGIADRIEYAQFLHDASEIKIFQNTFD
                     HSIETDNTVVLEIPIQKPNVEIPVIEIFLKDR"
     gene            complement(4268..5431)
                     /locus_tag="PLT86_09740"
     CDS             complement(4268..5431)
                     /locus_tag="PLT86_09740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT36933.1"
                     /translation="MKMRDSYDIVVVGGGPAGCEAALFAAQNGASVLVVEKDPDIGIP
                     VRCAEGISVRALREFYEPDPLFCRQHVNEYQLVAPNGTRVEVSGIGEGVILDRKVFDR
                     MVAEDAARAGAHIMVNASAVGASREGDGVVVHLEGLPDVRCRVMIGADGVESRAGRWL
                     GLRTACRPHDMETAAQFVLGGIDIVPTRFEMHFGTQLAPGGYLWVFPKGKGVANVGLG
                     ISGSHAGAATPLELLERAVQRLFSGASVLGRVLGGIPCSGGLKTIVGDNVMLVGDAAH
                     QANPLTGGGIATGLKAGRIAGRVAAEAIRSGDCSRRALQLYEDEWNDRLGNLHRRFYR
                     IKEAVFNIPDETLNDLADVVNKVPLEERTMNKILARSLAKKPRILLDLARVMF"
     gene            complement(5428..5643)
                     /locus_tag="PLT86_09745"
     CDS             complement(5428..5643)
                     /locus_tag="PLT86_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT36934.1"
                     /translation="MIRFARTTCDFCGVCVGVCPVDAISLFRAELLIDHAKCISCNFC
                     VDVCPSRSLHGEEEKDTRAENRPGVSP"
     gene            complement(5722..6315)
                     /locus_tag="PLT86_09750"
     CDS             complement(5722..6315)
                     /locus_tag="PLT86_09750"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOT36935.1"
                     /translation="MTVFVLLCVSSGVLVGFFGGGVIPGVDTAIVVLLVVLVFGVGLD
                     LGVRGDALSRVRSLGIRAIVIPVISCVGSLTGVALVASAFWGHPLKDAFALGSAFGWY
                     SLSGPMLTHLGGPELGALGFLSDVLRELLAFVLIPVVAGRIGSAEAVALGGATSMDTT
                     LPLIARTTNGEAAPLAFAHGMVVGLIAPVLIPFWFRF"
     gene            complement(6318..6611)
                     /locus_tag="PLT86_09755"
     CDS             complement(6318..6611)
                     /locus_tag="PLT86_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36936.1"
                     /translation="MQLILVLCMATGLGWFIGSRPVLGARLARSGHTVMLVAIALLLF
                     AMGYSLGSRPDIIAQAGRLGIEAVSLALAGASGAVVSVWLSRRWRNARNTRQE"
     gene            complement(6611..7420)
                     /locus_tag="PLT86_09760"
     CDS             complement(6611..7420)
                     /locus_tag="PLT86_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36937.1"
                     /translation="MIPTTRIGALEVSRLAIGGNPFSGISHQSRERDLAMRDFFTVAR
                     IKETLAKCEEHGINVLFGRADNHIMRTLVEYWNEGGRIRWFAQTTPERSSLHDVIRQA
                     AGNGASAIYIHGGTAKDFRDTGNWAGLAEAVDLIRSLGLPAGCATHQPDFHAERRAHG
                     VDVDFCLQSLYSLEGRRGKIDQHDEHEQFDDGDRLPALKAIAANPEPTFAYKVLAAGR
                     KDVGRSLTEVVTCLKEQDGILMGMCPDTNPGMVAENASLVSSLLRSLPESV"
     gene            complement(7417..8202)
                     /locus_tag="PLT86_09765"
     CDS             complement(7417..8202)
                     /locus_tag="PLT86_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HOT36938.1"
                     /translation="MRPIAEIVDAISRRTLTLDGTDHPVQIDENDISRVYLPLARLVR
                     ERSACVATRRYIVGISGPPGAGKSVSATILAACLEELGAGVVVIPLDGFHFPNEYLRT
                     HRGPDHAGEERSLADLKGWYPTFNVKRALDALEAVRAGQQVRLPVYSRVLHDPVEGGL
                     SIEPSHHVVLVEGNYLFFREGLWNRVYELLDLTAFVTAPREVLIAQLRERHMRGGRSA
                     REAEEKIRKVDEPNMDAILGTRNRAEFVIEGVRGSLAMERKGV"
     gene            complement(8202..>8773)
                     /locus_tag="PLT86_09770"
     CDS             complement(8202..>8773)
                     /locus_tag="PLT86_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT36939.1"
                     /translation="LQPEELDRGAVESSSMLYLDAREPFASVVAAGWAHAAGRKVFID
                     ADNVTEGIADVLPLCDTIIASQNFALKYFGDSDPERAARKLHERFRVVCGVTAGSAGS
                     YLATPEESFRQPAFEVDVVDTTGAGDVYHGAFAVGLLRRWPLRDTACFASAVAAMKCR
                     ELGGRSGIPRFAEACAFLNERGMHGAWEN"
BASE COUNT         1896 a         2782 c         2392 g         1703 t
ORIGIN      
        1 atgagttggt cgccataagt gctgccggaa tcagcgtata ctgggttgcc ctgccgttgc
       61 tgaggctcgg attgcttctg tcactcgcgg cgctgttgct caccgagctt gtcgtccccg
      121 gcgcaaatga cgaacgcagc agaattctac agctcgcacc gcagggtatt ctgtctggac
      181 atgccttcca cgtgtgcagg caggacagaa gcggcctgat tctctacgca gaacggtaca
      241 acatcgcggc gaaacgggcg caacgtgtcg caatcgtgca gcttcgaggc ggggtccccg
      301 tacgccggat cgacgcgtcg gagatggtat gggatgacct gggatggcaa cttcagagcg
      361 cgctggacag gtcctttctg ggggacagca tggtgttcgc ctcgcgggag gcgatgaggt
      421 tgccgttgct cacaatccaa ccggaggacc tggcgcgcgt tgacaagcta ccggaacaga
      481 tgtccttcct tgagcttcgc gattacatcc ggcgcaccag gttggtcggc ggcgatgctt
      541 ccagatggct cgtggacctt catctgaaat tcgcgttccc gttcgcgaac cttatgatgg
      601 tctggatcgg cttccctctc gcagcccgct cgtggcgagg tggacgcgca gcgtatgtcg
      661 gcctcacgtt cctcgtcggg ttcctctact tcgtcgtagt tcgctccggg caggcgctcg
      721 gacgatctgg agatctccac cccgttgttg cggcatgggg agcaaatgcg ctcttctttg
      781 tgatcggcgc cgcgcttttc cgctggtcga ggaagtagct cactccctcc agaggaagta
      841 gtcccccggc tgcgcttcct tccccatcca cgagtatggc tcgccggaga aattcgcgag
      901 cacgcacgtt ccatcagaaa accttgagaa ctgaacatcc gccgaatgga agcccttttc
      961 tgtgtccacg cgaggtggag ccaggaagcg gtgctcaacg agcgcagcgt agcttgtctc
     1021 ccgcatcgtc ttgcttatcc gtagggagaa ctcgtcggtt tcgtccggca tcaaacccca
     1081 gagaggaacg tagagcggat cgcaggtgta gaaatccgtg gacttcggtg tcatgttcag
     1141 cacggaacca tggtacacca tgacaaacaa tgggatagga atggcaggag ggatcaataa
     1201 cggtttgtca ctgcacccgt agccgatgaa ctggtacacg ggaaaatacg ccccgacgtc
     1261 aacgatatcc gcgaggaaat ccatcggcat ggattccgtc gcaaccgtgc cgaacagatc
     1321 ccggacatgc gaaagcagct ctcgacgcat gcctgcatct tgttctcgcg taagaggatg
     1381 caacgaatcg aagcactcgt tcaactccat cgcgccctcg acgtcaatgt agatcgaacc
     1441 ttcgccgatg agttccttca ggtgcggatg atcacggcgt gaagtctcca gccggcacac
     1501 cgagcagaca gtactcgacc gcatcttcaa atgctccgca taccagttcg ggcgtggcga
     1561 cccgtcacgc aacttggcca tgatcgcaag gtcgtacccc ggggtcatag gccacatatc
     1621 gatgaggttt tcgtacaagc ttaagtaata cgccttgtca accgaccgtg cgtactgcgc
     1681 gcctcgcgcg aggtcctcct cagtcccccg attcggcggc actgcgttcg ttccgaagaa
     1741 cggccgctgc cagatgttgg ttgcgtacga caccagaccc gggaagccca tccgttttgc
     1801 ggtgtcggca agttcttctg gcgttcgccc gccacctgtt ccgttgaaat caagggccat
     1861 ttccgcaagc acagagcccg gcaaggtgtg gaccagggga tgaccctcgg ctttctcctc
     1921 cagcgtcctg taacgcttct cagcctgcaa aaccgaccga taccacgatg caagctcgcc
     1981 gtagcccccc tcaaaggcac ggtagtctgc ccgccgtgag tagtccaggc gccccgcttc
     2041 gaacagaaaa cccggcgaaa ccgcatatgc cgggggatct cccgagttga tctcgccgac
     2101 caggtagcaa tcgaacggtg ttttgacaat tgttccaagc gtggcaacgc cgtcgcatat
     2161 gccaaacaac ggcatcgagt agccgttctg gccgccgctg tagatcaggt tttccagcgg
     2221 gaaagcacct tctcgctgtg agaaatccac cattatccct gcgccccgcg gcaatatgag
     2281 tcccccgccg ggaaccggtt cctttccggg agcctgaacg aagccgaggc ggtacggaaa
     2341 atcgacgatg atctcggcac cggccggcag gccggtcacc ctctccacct cgaacctcac
     2401 gcggtcgcgc tcgatgatga tcgcgcactc cactccaaca cgcccgtaac acgtctggag
     2461 gccctccagc gccactccaa gccgcctgcc ctcggcctct gccgaaaggc acgatgaatc
     2521 ccattcgacc aattcatatg gttctggaaa gccccagtaa acgggtggtt tgccggtggg
     2581 gcgaggtatg cgaagacgga tgaaaggctt cccggaaatc cacccggcac ccagagcgag
     2641 atcccgcaat gccagcgtga aatcattcgg acgaatctct accgcgtacc gctcgtacgt
     2701 tatccggcaa accgaatcgt attccggcat ggtcggctcc cttcctgttc tggttcgaca
     2761 tggtacccga agaataggcg gccaaagcag gttcggcaac acctccccgt tgacgccgtc
     2821 agcgcacgtt cacatgtttt cgacgtcact tcgtagtgcg tcctctactt caaggagatc
     2881 acatcatgcc gcagatgccg gaaaaaacac ctggaaaaac cgactggttc atccatgaca
     2941 ggtttggaat gttcattcac tggggactgt atgccctgcc agcgcggcac gagtgggtga
     3001 aatcgcggga gatgatacct gacgagaaat acgacgtgta cttccgccat tttaaccctg
     3061 acctttatga cccgaccgta tgggcgaagg cggcgagaaa tgccggcatg aagtacttcg
     3121 tcgtcaccac caagcaccac gaaggtttct gcctgtggga ttccgacctg acagattaca
     3181 aagcaacaaa cactccctac gggaaagacc ttttgaagcc catggtggag gcgtttcgcg
     3241 cggaggggct gaaagttggt ttctaccatt cgctgataga ctggcaccac ccggagtttc
     3301 ccgtcgactg cacgcatccc atgaggaacg acaaagagtt ccgcgaacgg gcaaaagacc
     3361 gtgacatcac caagtatgcg gaatacctcc acggccagac ccgggagctg ctgacccgtt
     3421 tcggaaagat cgacatcatg tggttcgatt tctcataccc cacccagccg gacgggggaa
     3481 agggacggaa cgactggcaa tccgagaagc tctacgaact tgttcggaag ctgatgcccg
     3541 acgtcgttct tgacgaccgg ctcgatctcg agaaagggta tgacatcaaa acgccggagc
     3601 agtggcaacc gcaggagtgg gtgaaggttg atggaaaacc cgtggtgtgg gaggcctgtc
     3661 agactctgaa tggaagctgg ggatatgacc gggacaacct cgactggaaa tcgccggcgc
     3721 ttctgttgtg gatgcttgtt gacagcgtgt ccaaaggagg aaacctgctc ctcaacgtgg
     3781 gtcccacggg caggggtgag tttgacgaac gcgcgcttgc cactctgcag gcaatcggga
     3841 agtggacgcg ggtaaatggt cgatcaatat acggttgcac cgcaagcgat tacacgccac
     3901 cgccagattg ccgctacact cagaatggca accggcttta tctccacgtg atggcgtggc
     3961 ctctgcggca tctgcatctc aagggaattg ctgacagaat cgagtacgcg cagttcctgc
     4021 acgatgcgag cgagatcaag attttccaga acacgttcga ccactccatc gaaacggaca
     4081 acacggtagt tctggaaatc cccatccaga aaccgaacgt ggaaatcccg gtaatagaaa
     4141 tcttcctcaa agaccgataa tggcgtgaag cactctggcg aaggggacgc agcttgcgag
     4201 ttgcgtcccc tttttttcgg gggaatttac ggcaaggagt gtgcccgtcc cgaaatccgg
     4261 tcgaggttca gaacattacc cgggccagat cgaggaggat tcggggcttt ttcgcgagcg
     4321 atcgcgcgag gattttgttc atggtccgct cttccagcgg aactttgttc accacgtcag
     4381 ccagatcgtt cagggtttcg tcgggaatgt tgaatactgc ctccttgatt cggtaaaacc
     4441 tcctgtgcag gttcccgagc cggtcgttcc actcatcctc gtacaattgc agcgcccgtc
     4501 gcgagcagtc acccgaccga atcgcttccg cagccaccct tcccgcaatc cttcccgctt
     4561 tgaggcccgt cgcgatgccg ccaccggtga gcggatttgc ctggtgggct gcatctccta
     4621 ccagcatcac gttgtcgcct acaatcgttt tcaagccacc cgagcacggg atcccgccca
     4681 gcacgcgccc gagaactgaa gcaccggaga aaagcctctg aaccgcgcgc tccagcaact
     4741 ccaacggagt ggcggcccca gcgtgagagc cgctgattcc cagaccgaca ttcgcgacgc
     4801 ctttcccttt cgggaatacc cagaggtaac cgcccggggc gagttgtgtt ccgaaatgca
     4861 tctcgaatcg cgtcggaacg atatcaatcc cacccagcac aaactgggca gccgtttcca
     4921 tgtcatgggg acgacacgca gtccgcaggc caagccaacg gcctgcccgg ctttccacac
     4981 catcagcgcc tatcatcacc cggcagcgca catccggcaa accctccaga tgaacgacaa
     5041 ctccgtcacc ctccctggaa gccccgacgg cgctcgcatt caccatgatg tgggcacccg
     5101 ccctcgccgc gtcctccgcc accatccggt caaatacctt ccggtcgagg atcacgcctt
     5161 ctccaatccc gctcacttct acccgtgttc cgttcggggc aaccagctgg tattcattca
     5221 catgttgtcg gcagaacaga gggtccggtt cgtagaattc gcgaagtgcc ctgacgctga
     5281 tcccttccgc gcaccggacg ggaattccga tgtccggatc cttctccacc acaagcaccg
     5341 acgcaccgtt ctgcgccgca aacagtgcgg cctcgcaccc cgcgggaccg ccacccacca
     5401 caacgatgtc gtatgagtcg cgcatcttca tggcgagact cccggccggt tctcagcccg
     5461 tgtatccttt tcctcctcac cgtgcagtga acgggaagga cacacgtcga cgcagaagtt
     5521 gcaggaaatg cacttcgcgt ggtcgatcaa caactccgct ctgaaaaggg agatggcgtc
     5581 aacggggcat acgcccacgc agacgccgca gaaatcacag gtagttcgtg cgaaacgtat
     5641 catgcctgtt caccgatggg tcacggtggg actcatcaca cgggttcgga aaagtaatcc
     5701 ttcgctgctc gcggtggcaa gttagaatcg gaaccagaaa gggatgagca ccggcgcaat
     5761 caggcctacc accatgccat gggcgaaggc gagaggtgcg gcttcgccgt tcgtcgttcg
     5821 ggcaatcagg ggtaacgtcg tgtccatcga tgtcgcgcct ccgagcgcca ctgcctccgc
     5881 agagccgatt cggcccgcca caacgggaat caaaacaaac gcgaggagtt ctctcagcac
     5941 atcggaaagg aacccgagag ccccgagttc cggcccaccc aaatgagtca gcatcgggcc
     6001 cgaaagactg taccatccaa acgcgcttcc aagtgcaaat gcgtctttga gagggtgtcc
     6061 ccaaaaggcc gacgccacca gcgcaacacc cgtcaagctc ccgacacacg aaatgaccgg
     6121 gatcacaatc gctcgaatcc cgagcgagcg caccctcgaa agtgcgtcgc cacgcacccc
     6181 gaggtcaagc ccgacgccga aaacaagcac gacgaggagc acaacgattg ccgtgtcgac
     6241 gccgggaatt acacctcccc cgaaaaagcc aaccagcaca ccggagctca cgcacaatag
     6301 cacgaaaact gtcacattca ctcctgccgg gtattccgcg cgtttctcca ccggcgcgac
     6361 agccacactg aaacgaccgc tccggaagcc cccgcaaggg ccaggctcac agcctcaata
     6421 cccagccttc cggcctgtgc aatgatgtcc ggacgcgaac cgagcgaata acccatcgca
     6481 aacagcagca acgcaatcgc gaccagcatg accgtgtgac ccgaccgcgc cagccgcgcg
     6541 cccagaacag gacgacttcc gatgaaccac cctaggccgg ttgccataca cagaacgagg
     6601 atcagttgca tcagacgctt tctggcaagg agcgaagcag gctgctcaca agtgaagcat
     6661 tctccgccac cataccgggg ttcgtgtcag ggcacatccc catgagaatt ccgtcctgtt
     6721 ctttcaggca ggtaacgact tccgtcaatg aacgcccgac gtctttccgt cccgcggcaa
     6781 gcactttgta cgcgaatgtc ggttcagggt tggcagcaat agccttcagc gcgggaaggc
     6841 gatccccatc atcaaactgc tcatgctcgt cgtgctgatc gatcttcccg cgtcggccct
     6901 cgaggctgta aagcgactga agacagaagt ccacatccac cccgtgtgct cgcctctcag
     6961 cgtggaaatc gggctggtgc gttgcgcagc cggcgggcag tccgagagag cgaatgaggt
     7021 caacggcctc ggcgaggccc gcccagttgc cggtatcgcg gaaatccttc gcggtgccgc
     7081 cgtggatgta gatcgccgat gctccgttgc ccgcggcctg ccggatgaca tcgtgcaggg
     7141 aggatcgttc gggagtagtc tgcgcaaacc agcggattcg ccctccttcg ttccagtatt
     7201 ctacgagggt gcgcatgatg tggttgtcag cacgtccgaa aagaacgttt atcccgtgtt
     7261 cttcgcattt tgcgagcgtt tccttgatgc gggccacggt gaagaagtcc cgcatcgcaa
     7321 ggtctctttc ccgactttga tgcgaaattc cgctgaacgg attccctccg atggcaagcc
     7381 gactgacttc cagcgcgccg attcgcgtcg ttggtatcat actcccttcc tttccatcgc
     7441 gagggaaccc cgcacgccct cgatcacaaa ttcggcgcgg ttcctggttc cgagaatggc
     7501 atccatgttg ggctcatcaa ccttcctgat cttttcctcc gcctcccgtg ccgagcgccc
     7561 gccgcgcatg tggcgttcgc gaagctgcgc gatgagaacc tctcttggcg ccgtcacaaa
     7621 cgcagtaagg tccagaagtt cgtacacccg attccacagg ccttctcgga agaaaaggta
     7681 attcccttct accagcacca cgtggtgcga tggctcaatg gaaagccctc cttccacggg
     7741 gtcgtggagg acacggctgt ataccggcag acgcacctgt tgacccgccc gaacagcctc
     7801 cagtgcatcc agcgcgcgct tcacgttgaa cgtcgggtac cagcccttca aatcggcaag
     7861 cgatcgctcc tctcctgcgt ggtccggacc tcgatgggtt ctgaggtatt cattcgggaa
     7921 atggaatccg tccaaaggaa tgacaaccac gcccgcgccc aattcttcca gacacgcggc
     7981 cagaatggtt gcactgacgc tctttccggc gccggggggt cccgaaatgc caactatata
     8041 gcgccttgtc gcgacgcacg cggatctttc acgtaccagc cgtgcaagcg gcaaatacac
     8101 acgggaaatg tcattttcgt caatctgcac cggatgatcg gtgccgtcca gagtcagtgt
     8161 acgtctcgag atcgcatcca cgatctcagc gatcggccgc atcagttctc ccacgctccg
     8221 tgcattcccc gctcgttcag gaatgcacac gcttccgcaa aacgcggaat tcctgacctt
     8281 ccccccagtt cccggcattt catcgccgcc accgccgacg cgaagcacgc agtgtcgcgc
     8341 agcggccatc tccgcaacaa acccaccgcg aatgctccgt ggtagacgtc acctgctccc
     8401 gtagtgtcaa cgacatcgac ttcgaacgcg ggctgccgga atgattcctc cggtgttgcc
     8461 agatacgacc cggcggatcc ggccgtaact ccgcacacca ccctgaagcg ctcatgcaac
     8521 ttccgggcgg ctcgctctgg atctgaatcg ccgaagtatt tcagcgcgaa gttctgtgac
     8581 gcaatgatcg tgtcgcacag cggcagtaca tcagcgattc cctcggtaac attgtccgcg
     8641 tcaatgaaca cttttcgccc cgccgcgtgc gcccaacccg ccgcgacaac tgacgcaaat
     8701 ggttctcgcg catccagata caacatcgag ctcgattcca cagcaccccg atcaagctcc
     8761 tccggttgca gaa
//
LOCUS       DAOSOO010000133         8751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8003_length_8751_cov_5.325667, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000133 DAOSOO010000000
VERSION     DAOSOO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_8751_cov_5.325667"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLT86_09775"
     CDS             complement(<1..930)
                     /locus_tag="PLT86_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT36940.1"
                     /translation="MSCSVVNLTKALVAIPSESSRSNLPVMQQLCTFLYSQGFRVTRY
                     PQNGPSTGPEKTNLIAERGNGTRRLVFSGHLDTVPVGERGQWQHDPYGAEGIIGDRLY
                     GRGTVDMKGQVAAMACACASVPEEVLRNLTLTLAITGDEEIGHVGIKDLAAKHVFQRA
                     VGCVVGEPTGLRVVSAHKGGMTVRITVRGLSCHSSKPHLGVNAIEQAVRLLNLLGERL
                     APWCAQRHPAFGEEPPTFTVVGVSGGVADNVVPDSCTVNISARALETGHFETFRAALE
                     GIISELVDNDTRKGVPPERRFSADMDLLKWAPPM"
     gene            complement(950..1651)
                     /locus_tag="PLT86_09780"
     CDS             complement(950..1651)
                     /locus_tag="PLT86_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36941.1"
                     /translation="MQNPPPQEHRAASLSARLLLRDTVRAYLRFLPVSAAVVALRLVP
                     FYVLFWFLPPGTDAVHAVMETAVAVPCVAALVRIVWNGSDAARPRFADSLLGASPVAT
                     LKLLVTNLFLLTVALMVAQISPEWFLGFVIVWTWAALLTDQVVLIEGKMMITAIARSY
                     GIVRRAWKLSLLGLLAVLLPEALNLTITEFVPEALKRELLTRGVTVLFLPFSTTVLTL
                     LYERTESLAAPPFDG"
     gene            complement(1738..2307)
                     /locus_tag="PLT86_09785"
     CDS             complement(1738..2307)
                     /locus_tag="PLT86_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT36942.1"
                     /translation="MRNGLLSAFLVVGLACDAMAQGGYIPVIYAANKEYGLSGSYLKQ
                     KDAADNGTSDLTLMGHWGIMMPLGLQPELEAGWRKSTTDVPDFQARDYTLAGNLLWNL
                     SPFNSLSFFGLAGYGYIGAKTETKVGTVTTTLTDNRWFSQFGGGVKWLLVPNAGIRLD
                     YRYQNGATLNAAGNKQSRHVLLLGVSVFQ"
     gene            complement(2340..3830)
                     /locus_tag="PLT86_09790"
     CDS             complement(2340..3830)
                     /locus_tag="PLT86_09790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT36943.1"
                     /translation="MAAKNTVLVAHHDSATRDLFREALDKEVFDTIITDKGTEARELF
                     ETLGDRLDALVLDSQLPDGDPYELLAEFRKGTSDAEIVVVVNEVNEDAFSRALGAGAF
                     DFLVQPFSKLAAQQMLRNAVERRRCRRLTADLVSTKRSLEVELTVKSFQIDQVRELVN
                     FSRLVNEHLRLDAILDITFERLPELVNAETFSLFLYEPSRNEFHLAITNRPNLDPREP
                     IVVKEENSPIMKRAIETHQVLFIDDVLKSGLLPDEQARTAKSRHYKKKHSVCLPLKVE
                     DRPIGVLNLNDFRAEEQIQDYINVAVLAAAFLAPVISNARLYEQVEETANRDGLTQLY
                     NRAFFDKNLGENFRRAARYGRPLSLLFLDIDHFKQLNDTYGHQAGDMVLRRVAARLLD
                     NSRKGIDVAARYGGEEMVLIGPETPADGIKIVAERIRQDIEAMRVKFRNESGEKTLSV
                     TVSIGTATYLPGVSDYPSERALTEAADSAMYQAKHSGRNRCSHHNG"
     gene            4070..5371
                     /locus_tag="PLT86_09795"
     CDS             4070..5371
                     /locus_tag="PLT86_09795"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT36944.1"
                     /translation="MAGSVQVGLVGLGTIGVGVARLLLEKRSKVFAGDCGPITLKRIA
                     DKDLSDRGIDLPSGILTEEVRAVLEDPEIGIIVELVGGIEPARTFVLTAIEHGKHVVT
                     ANKALLSAQGEEIFRAAQKRGVDVLFEAAVGGSIPIIRALRESLGTSAIRSIYGILNG
                     TTNYILTRMTDDGASYDEMLKAAQQLGFAERNPVADVSGKDSLQKLVILARLAFGASL
                     SPEEILCEGIERITPMDISFAQELGYRIKLLAIAKEREGRIEARVHPAMIPLSGLMAN
                     VKFEYNAIEVVGEEFGTQVFYGKGAGQRPTATVVVSDVIDLALRIQSGAPTCRVAPLL
                     ASASSVRLVTAEDLFLRHYVRLEVFDRAGVLADIARLFAEENISIESVIQKGRAASDT
                     VPLVIMTHEASEASIERALRRLSTLNVVRGPIQRIRVEELE"
     gene            5374..6495
                     /gene="thrC"
                     /locus_tag="PLT86_09800"
     CDS             5374..6495
                     /gene="thrC"
                     /locus_tag="PLT86_09800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT36945.1"
                     /translation="MEPDVQNPHSAAWPGIIQRYRPHLPVTAGTPVITLLEGNTPLIP
                     AVALPRLFGADIELYFKFEGMNPTGSFKDRGMTLAISKAAEDGAEIVVCASTGNTSAS
                     AAAYASRAGMISTVLIPRGNIAQGKLAQAMMHGAVVIQIDGNFDDCLRLVRALAEKRP
                     VAIVNSINPYRIEGQKTGSFEICEQLGGRPPDYHVLPVGNAGNITAYWKGYREFFDNG
                     FIDRLPKMIGFQAAGSAPIVRGSRIEKPETIATAIKIGDPASWKSAVAARDESGGLID
                     EVTDEEITRAQKLLARYEGVFSEPASAASVAGVAKLSRKGFFPKGARVVCILTGHGLK
                     DPERAIAIAEAPVRSSANIDAVLDLLDRAVEARRRHSNP"
     gene            6514..7746
                     /locus_tag="PLT86_09805"
     CDS             6514..7746
                     /locus_tag="PLT86_09805"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT36946.1"
                     /translation="MGLIVMKFGGTSVATPELITRVARRVLATKKEGNDVVVVVSAMG
                     DATDELESMAAKISVEPAEREMAILMITGERVSAALLAIALNELGQPARAFTGWQAGI
                     VTDSASPLRAKIRRIEATRIREALREGSVAVVTGYQGVTEDGDETALGRGGSDLSAVA
                     LACALKADACEIYTDVDGVYTTNPRLVKDARKIRAISYEEMLELASMGAQVMQARSIE
                     YAKKHGTVIHVRSSFSEAPGTVIREADTAMEAVVVRGIAYDMTEAKITVRGVTDRPGV
                     AATLFNRMAQAGINVDMIVQNVSEDGITDLSFTVTRPDLKKAMDACLLIQRDIGAREI
                     VADEEIAKISLVGIGMRSHTGVAAKMFDTLACHGINIEMISTSEIKISCIVREAQAET
                     AVKALHTTFALDREGDEY"
     gene            7772..7843
                     /locus_tag="PLT86_09810"
     tRNA            7772..7843
                     /locus_tag="PLT86_09810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7804..7806,aa:Lys,seq:ctt)
     gene            8017..>8751
                     /locus_tag="PLT86_09815"
     CDS             8017..>8751
                     /locus_tag="PLT86_09815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT36947.1"
                     /translation="METRTLGRTGTQLSIIGFGGIVVAGKTQAEADRLVSFALDSGVT
                     YFDVAPTYGDSEERLGPALQGKREGIFLACKTTERTREGAQRELERSLRRLRTDHFDV
                     YQLHGVSSMQEVETIFAPGGAIETIERAKTEGKTRLAGFSAHSEEAALALLNRYQFDT
                     VMFPVNWVCWSNAGLGARLVHTAKERGMGILAIKAMARTKWPENGRRDEYSACWYQPE
                     DDPATASLALRFAWSEGVTACIPPGDA"
BASE COUNT         1898 a         2429 c         2552 g         1872 t
ORIGIN      
        1 catcggcggg gcccacttca gcaggtccat gtccgctgag aaccgccgtt ccggaggaac
       61 tccctttcgt gtgtcattgt caacaagctc gctgatgatc ccttccagtg ccgctcggaa
      121 tgtttcaaag tgccccgttt cgagtgctcg ggcgctgata ttgacagtgc atgaatcagg
      181 tacgacgtta tccgcaacgc cgcccgagac gcccacgacc gtgaaggtgg gcggttcttc
      241 tccaaaagcc gggtgacgtt gcgcgcacca gggtgcgagg cgttctccga gaagattcag
      301 taaccggaca gcctgttcta tggcgttgac accgaggtgc ggtttgcttg aatggcaact
      361 aagacctcga acggtaattc ggacagtcat cccgcccttg tgcgcactca ccacgcgcag
      421 accggtaggc tcacccacca cgcaccccac ggcgcgctgg aaaacatgct tggcagcgag
      481 gtccttgatc cctacgtgac cgatttcctc gtcccctgtt attgccagcg tcaaggtcag
      541 attcctcaac acctcttccg ggacagatgc acacgcgcac gccatcgccg ccacctgccc
      601 cttcatgtca accgttccgc gaccgtagag acggtcacca attatccctt ctgcaccgta
      661 aggatcatgc tgccactgcc cgcgctctcc aacggggacc gtatcgaggt gaccggaaaa
      721 cacaaggcga cgagttccgt ttcctcgttc tgcaatcagg ttggtttttt ctggtccggt
      781 tgacggaccg ttctgcgggt atctggtgac gcggaagcct tgcgaataca ggaacgtaca
      841 gagttgctgc atgacgggga ggttggatcg cgagctttcg ctcgggatcg cgaccagtgc
      901 cttcgtcagg ttcacgacgg aacagctcaa gtccttacta tccttttcgt caaccgtcaa
      961 acggaggtgc cgccagcgac tccgtgcgtt cgtacaagag agtcaacact gtcgtcgaaa
     1021 acgggagaaa aaggacggta actccgcgag ttagcagctc ccttttcagc gcctccggga
     1081 cgaactccgt gattgtcaga ttaagcgctt cgggcagcag aaccgccaga agccccagaa
     1141 gagacagttt ccacgcacgt cgcacaatgc cgtacgaacg tgcgattgcc gtgatcatca
     1201 ttttcccttc gataagcacg acctggtccg tcagaagtgc cgcccatgtc cacacgatga
     1261 cgaatccaag gaaccactcg gggctgatct gcgccaccat aagtgcgacg gtgagcagaa
     1321 aaaggttcgt cacaagcaac ttcagagttg cgactggaga cgccccgaga agggaatcgg
     1381 cgaaacgagg acgtgctgca tcgcttccgt tccacacaat cctgacaagg gccgcgacgc
     1441 agggaaccgc gactgccgtt tccattaccg cgtgtaccgc gtcggtgccc ggaggaagaa
     1501 accagaacag aacgtaaaaa gggacaaggc gcagcgccac aactgctgca gagacgggca
     1561 gaaaacggag gtatgcgcgg actgtatctc gaagaagcag tcgagctgaa agtgaagccg
     1621 cgcggtgctc ttgcggcggg ggattttgca tttcgttcta ctcgatcaca gggaacagac
     1681 gcgcacgttg ctccactacg aggcaacttt ctattgccgg atgggcaaac cagccgttta
     1741 ctggaaaacg gaaacgccga gaaggagtac gtggcggctc tgcttattcc ccgcggcatt
     1801 cagtgtggca ccgttctggt agcggtaatc caaccgaatc ccggcgttcg gcacgagaag
     1861 ccacttgact cctccgccaa actgcgaaaa ccaccggtta tccgtaagcg tcgtggtaac
     1921 cgtcccaact ttggtctccg tcttcgcgcc tatgtacccg tagccagcca gaccgaagaa
     1981 agaaagggaa ttgaatggac tcagattcca caggaggttt cccgccagtg tataatcccg
     2041 tgcctgaaaa tccggaacgt ctgttgtgga tttgcgccac cccgcttcca gttccggttg
     2101 caggccgaga ggcatcatga ttccccagtg gcccatcagt gtcaggtcgg atgttccgtt
     2161 atccgcggcg tctttctgtt tcaggtagct gccgctcaat ccatactctt tgttcgccgc
     2221 gtaaatgaca gggatgtacc cgccctgagc catggcgtca caggccaacc cgacgacgag
     2281 aaaggcactc agcaatccgt tcctcattcg acacgctcct ttgtccatcg cgggacgtat
     2341 tacccattat ggtgactgca gcggttacgg cctgaatgct tcgcctggta catagcgctg
     2401 tccgccgctt ccgtaagagc acgttcgctc gggtaatcgg acacacccgg aagataggtc
     2461 gccgtcccga tacttacggt gacggacagc gtcttttctc cggattcgtt gcggaatttc
     2521 acccgcatcg cctctatgtc ctgcctgata cgctcggcca cgatcttgat gccatccgcc
     2581 ggcgtttcag ggccaatcag aaccatttcc tctccaccat aacgagcagc aacgtcaatc
     2641 cctttgcgcg aattgtctaa aaggcgggcc gcaacgcggc gcaggaccat gtccccggcc
     2701 tgatggccgt acgtgtcgtt aagctgcttg aaatggtcta tgtccagaaa caacaacgaa
     2761 agaggtcgtc cgtaacgagc tgcacgcctg aagttttcgc cgagattttt gtcgaagaaa
     2821 gcccgattgt acaactgcgt caatccgtcg cgattcgccg tttcctcgac ctgttcataa
     2881 agccgggcgt tcgaaatcac cggtgcaaga aacgccgctg ccagaaccgc aacattaatg
     2941 taatcctgaa tctgctcctc ggcacggaag tcgttaagat tcagcacacc gatcgggcgg
     3001 tcttcgactt tgagcggcag gcagactgag tgctttttct tgtaatggcg gcttttcgct
     3061 gttcttgcct gttcgtcggg caacagacca ctcttcagga catcgtcaat gaacagcacc
     3121 tggtgggtct caattgcgcg tttcatgatc gggctgtttt cttctttgac gactataggt
     3181 tcgcgcgggt cgagattggg gcggttggta atcgcgagat ggaattcgtt tcgcgagggt
     3241 tcgtacagga aaaggctgaa tgtttcggcg ttcacaagtt cgggaagtcg ttcaaatgta
     3301 atgtcgagaa tcgcgtccag ccgcaaatgc tcgttgacga gcctgctgaa gttcaccagt
     3361 tcacgcacct ggtcgatttg gaacgacttt acggtgagtt ccacctcaag gctgcgtttc
     3421 gtacttacaa gatcggcggt gagtctcctg catctccggc gttccactgc attccgcaac
     3481 atctgttgcg ctgccagttt gctgaacggt tgcaccagaa aatcaaacgc tcctgcgccg
     3541 agcgctctgg aaaaggcgtc ttcgttaact tcgttgacga caacgacgat ttcggcgtca
     3601 gaggtacctt ttcgaaactc cgccagcaac tcgtacgggt caccatctgg caactgggaa
     3661 tccagaacaa gcgcatccaa acggtcgccg agcgtttcga aaagctccct tgcctcggtg
     3721 cctttgtcag tgatgatagt gtcgaaaacc tccttgtcga gcgcttcccg gaacaaatcc
     3781 cgcgttgcgc tgtcatggtg agctaccagc accgtgtttt tggcggccat gcggtcggtc
     3841 tcgttctgtt gcgtcgtgcc cgggagcaaa aaggctctat tgggaattaa gtttcgctcg
     3901 ttgatgatac gcgtaagtaa ttgaaccggt caagagtatc tcaattgcta cgggctgcga
     3961 tcctgcgggt ttgccccgcc ttgaccctcc gcggagcgga gtggtacgtt ttagaacgtt
     4021 tttcacgttc tttttgcgct tccgtggtct atttctagaa ggcacctcga tggcgggtag
     4081 tgttcaagtc ggtttggtag gtcttggaac cattggagtc ggcgttgcgc gtctcctgct
     4141 tgaaaaacgg agcaaggtct tcgccgggga ttgcgggccc atcacactga agcggattgc
     4201 tgacaaggac ctttcagatc ggggtatcga tcttccttcc ggcattctga cggaggaagt
     4261 aagggccgtg ctggaagacc ccgaaatcgg cattattgtc gaactggtgg gaggaattga
     4321 accagcgcgg acctttgtgc ttacggcaat cgaacatggc aaacatgttg tgacagcaaa
     4381 caaggccctt ctctctgcac agggagagga gatattccgc gcagcgcaga agcggggcgt
     4441 ggatgttttg tttgaagcgg cagttggcgg cagcatcccg attatccggg ctctcaggga
     4501 aagccttggt acgagcgcca tccgctcaat ttacggcatc ctcaacggca ccacgaatta
     4561 tatcctcacg aggatgacag acgacggggc atcgtacgat gaaatgctga aagccgctca
     4621 gcagctgggt ttcgccgagc gcaatcctgt ggccgatgtg tccggaaagg attccctgca
     4681 gaagcttgtc atccttgcgc ggctcgcttt cggcgcttct ctttcgcccg aggaaatcct
     4741 ttgcgaaggt atcgaacgca ttacacccat ggatatctcc ttcgcgcagg aacttgggta
     4801 ccgaatcaaa ctgctcgcca tcgctaaaga gcgtgagggc cggatcgagg cgcgcgttca
     4861 tcctgccatg atcccccttt ccgggctgat ggcgaacgtc aagttcgagt acaacgcgat
     4921 agaggtggtc ggcgaggaat tcggaaccca ggtcttctac gggaaaggag ccgggcaaag
     4981 gccgacagcg acggttgttg tttcggacgt cattgatctg gctttgcgaa tccagtcagg
     5041 tgctccgacg tgtcgcgtcg caccccttct cgcgtctgcc tcgagcgtcc gactcgtaac
     5101 cgcggaagat ctctttttga ggcattatgt ccggctggag gttttcgatc gggcgggcgt
     5161 acttgccgac attgcgcgat tgttcgcgga agagaacatc agcatcgaat cggtgatcca
     5221 gaagggacgt gcagcttcgg acaccgtgcc tctggtaatc atgacacacg aagccagtga
     5281 ggcttccatt gaaagggcgc tgcgacggct ttcgacgctg aatgtcgtcc gcgggcctat
     5341 tcagcgaatt cgcgtggaag aactggagta acattggaac ctgacgttca aaatccacat
     5401 tcggccgcgt ggcccggcat catccagaga tacaggccac atctgccggt caccgcagga
     5461 acgcccgtca tcacgctgct tgaagggaac acgccgctga tccctgcagt cgccttaccg
     5521 cgactgtttg gcgctgacat cgaactctac ttcaagttcg agggaatgaa tcctaccggt
     5581 tccttcaaag accgggggat gacccttgca atctcgaaag cagcggagga cggtgcggaa
     5641 atcgtggtct gcgcttcgac tggcaacaca agcgcatccg cggcggcgta cgcctcgcgg
     5701 gccggaatga tctccacggt gctcataccg cgtgggaata tcgctcaggg caaactcgct
     5761 caggcaatga tgcacggcgc ggtcgtcatt cagattgacg gcaatttcga cgattgcctt
     5821 cggttggtaa gagccctcgc ggaaaagcgc cccgttgcga tcgtgaactc gatcaacccg
     5881 taccgaatag aagggcagaa aaccggttct ttcgaaatct gtgagcagct cggggggcgg
     5941 cctcctgact accacgttct gcctgtcggg aatgcgggta atatcaccgc gtactggaag
     6001 gggtaccggg agtttttcga caacggattc atcgatcgac tcccgaagat gatcgggttt
     6061 caggcggcgg ggtccgcgcc catcgtccgt ggatcgagaa tcgaaaaacc tgaaactatc
     6121 gctacggcaa tcaaaatcgg tgaccctgca agctggaaga gtgccgttgc ggcgcgcgat
     6181 gagtcgggcg gactgataga cgaagtgacc gacgaagaaa tcacgcgtgc gcagaaattg
     6241 ctggcgagat acgagggggt tttctcggaa ccggcatccg ccgcgagcgt ggcgggcgtt
     6301 gccaagttgt cgcggaaagg gttttttccg aaaggcgcgc gtgttgtgtg tatcctgaca
     6361 gggcatggcc tgaaggaccc tgaacgggcg attgcaatag ccgaggcacc ggtacggtca
     6421 tctgcgaaca tagatgctgt gttggacctt cttgaccgcg ccgtggaggc gcggagacgt
     6481 cactccaacc cataacaaag acggaaagct gctatgggat tgatcgtaat gaagttcggc
     6541 ggaacgtcgg tagcgacgcc ggaattgatc actcgcgtcg cacgccgggt cctcgcgacg
     6601 aagaaagagg gaaacgacgt ggtggtggtc gtctccgcga tgggggatgc gaccgatgag
     6661 ctcgagagca tggccgcgaa aatatccgtg gagccggcag agcgggaaat ggccatcctc
     6721 atgatcacag gggaacgggt ttccgccgcc ctgctggcga tcgcgctcaa cgaactcggg
     6781 caaccggcgc gcgccttcac gggctggcag gcgggaatcg tgacagattc ggcgtcaccc
     6841 ctgcgcgcga aaattcgtcg catagaggct actcgtatcc gcgaggccct gcgggaagga
     6901 tccgtcgccg tcgttacggg gtatcagggt gtgacggagg atggcgacga gaccgccctc
     6961 ggcaggggcg gaagtgacct ctccgccgtc gcgctggcat gcgcgctcaa agcggatgcc
     7021 tgcgaaatct acaccgatgt agacggcgtg tatactacga atccgcggct cgtgaaagac
     7081 gccaggaaaa tccgcgccat ctcgtacgaa gaaatgctcg aacttgccag catgggcgcc
     7141 caggtcatgc aggcaaggtc gattgaatat gcaaaaaagc acgggactgt aatccacgtt
     7201 cgttcatcat tcagcgaagc gccgggaacg gttattcggg aggcagatac cgctatggaa
     7261 gccgtagttg ttcgaggcat cgcgtacgac atgacggaag cgaaaatcac cgtccgaggt
     7321 gtcactgacc gtccgggcgt cgccgcaacg cttttcaatc gcatggcaca ggccgggatc
     7381 aatgtggaca tgatcgtcca gaacgtgtcc gaggacggca tcacggatct ctcgttcacc
     7441 gtgacgcgtc ctgatctgaa aaaggcgatg gatgcgtgtc tgttgatcca acgcgatatc
     7501 ggggccagag aaatcgttgc ggacgaggag atagcaaaga tttctctcgt tggtataggt
     7561 atgcgctcgc acactggtgt cgcggcgaag atgttcgaca cactcgcctg ccatggaatc
     7621 aatatcgaga tgatcagtac ttccgagatc aagatatcgt gtatagtccg tgaggcgcag
     7681 gcagaaactg ccgtgaaagc gctacacacg acgtttgctc ttgaccggga aggcgacgaa
     7741 tactgatatt taagctttgc agtttgcttt tgcctccata gctcaatggt agagcagcgg
     7801 actcttaatc cgttggttga aggttcaagt ccttctgggg gtactccttg aacggcacag
     7861 gtgtttcggt ctgcctgtgc cgtttttcgt ttgtctcccg tccttgtgtt ctcaacgcct
     7921 tattgcgcac gtttcgtggc gtgaccatat tccatgacga ccatccatca cgagaaatga
     7981 cgaggagttg cagcgcgggt ctggaggccg gttcaaatgg aaacgagaac ccttggtaga
     8041 actggaacgc agctttcaat catcggattc gggggaatag ttgtcgccgg caagacacag
     8101 gctgaagcgg acagattggt cagcttcgcg ttggattcgg gcgtaacgta tttcgacgtc
     8161 gccccgacct acggggattc agaagaacgc ctcggacccg ccttgcaggg caaacgcgaa
     8221 ggcatcttcc tcgcctgcaa aacgacagaa cgcacccgag aaggagctca acgcgaactc
     8281 gaacgttcgc ttcggcgcct gcgcacagac catttcgacg tgtaccagtt gcatggggtg
     8341 tcaagtatgc aggaggtgga gaccattttt gcccccggtg gagcgatcga gactattgag
     8401 cgtgcgaaga cggaaggcaa aacccgcttg gctggttttt ccgcgcactc cgaagaagcc
     8461 gcacttgcgc ttttgaaccg gtaccagttt gacacggtga tgttccccgt aaactgggtc
     8521 tgctggtcga acgcgggttt aggcgctcgc cttgttcaca cggcgaaaga acgcgggatg
     8581 gggattctcg cgatcaaagc gatggctcgc acaaaatggc cggagaacgg gcgccgagac
     8641 gagtactccg cgtgctggta tcagccggaa gacgacccgg cgacggcgag cctcgcgctg
     8701 cgtttcgcct ggtctgaggg cgtcaccgcg tgcattccgc cgggcgatgc c
//
LOCUS       DAOSOO010000134         8747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8008_length_8747_cov_6.254372, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000134 DAOSOO010000000
VERSION     DAOSOO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8008_length_8747_cov_6.254372"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLT86_09820"
     CDS             <1..392
                     /locus_tag="PLT86_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT36948.1"
                     /translation="VFRTNYATVGISNCFDGEFPESIRRMRLVGAEILLWCNAACGDS
                     HFGTSHRIHHSQAHAQANNMWVVCCNCVAENASGTSLIASPHAEPLVILPPDEEALAI
                     ATIDLAVVTSWEPWKSRVDGSGYKEKP"
     gene            389..2107
                     /locus_tag="PLT86_09825"
     CDS             389..2107
                     /locus_tag="PLT86_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOT36949.1"
                     /translation="MNQPNVILVITDDQGYGDLGCTGNPWIQTPNIDRFYGESLRLRD
                     FHVSPLCTPTRGAIMTGHHPVRNGAWATCWGRSILRRDEVTMAEVFAASGYRTGMFGK
                     WHLGDNHPYRPMDRGFQHVVAHKGGGVGQTPDFWGNNYFDDTYFHNGEPVPHEGYCTD
                     VWFAEATKFIEANADSPFFAYIATNAPHGPFLVAEKYRRMYEGNPEIPNAAFCGMVTN
                     IDENFGRLEARLRELGIADNTILIFMTDNGSDGGLVIGEGERIVRGYNAGMRGKKGSY
                     YDGGHRVPFFVRWPNGGIGGGKDINEMAFHVDLLPTFISLCGLKAPEGVKFDGISLAG
                     LIHRRETALPDRTQFLQYRQDTVPPEKWTNAVATKQWRLVRGVELYDIKADPGQENDV
                     AARYPEVVARLREAHERWWEEISPRLDEYSPISLGNDAENPTRLDAMDVMGDMAFSQN
                     YIVLAKKSTGKWTVDVERAGTYRFSLRRWPKELGLPIGAAVSPEEARSLTFSDGSGRC
                     TTIRPARARLRLFDRESVLPVDPDAPEVTFELDLAQTGVTVLEAWFVDADGEERGAYY
                     VYVERV"
     gene            2616..3686
                     /locus_tag="PLT86_09830"
     CDS             2616..3686
                     /locus_tag="PLT86_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT36950.1"
                     /translation="MTNRERFNRVMRYEPVDRLPLMRFEPHQVYAVERWRAEGLPAEA
                     DPDHYFRICRIVDAPVDLFPIPRIEQAILSETDADYVQVDRFGNVLRRKKDSPLMYCG
                     NIAHPVKNIADWRAYSRHYDPYAPGRIPEDLDAIAAELNACDAPVGICVFPWFFRLGF
                     YALGMEGFLAAFYEQPALIHEMFGFWSDLVLHTIRPVLKKVKVDVLYLTEDLAYKNAP
                     HVSPRMYEQFWLPYQNPIIEECRRHGVELVCLWTAGNIVPLLPLAMRNGINCTWPLER
                     VAGMDPAMLRKRFGRELLLAGGISKEALVAGPEAIDREIESLMPVIRDGGFIPALDDM
                     PPAEVPLAHFAHMIDALRRIEA"
     gene            3710..4618
                     /locus_tag="PLT86_09835"
     CDS             3710..4618
                     /locus_tag="PLT86_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT36951.1"
                     /translation="MVTSQEGRDYYQKLCETPSFGANGFDFDGLRRGMATRREPTIPD
                     VQCIRMEINRIPCEWVMAKDADPAVRLLYLHGGGFISGSGAFYLPLAAHISAAAKCMV
                     LLPDYRLAPEHPFPAALEDCLSIYQALMEIEPEDEDSRTATFIAGDSAGGGLTLSTLL
                     ALRDRGMAMPDGGIALSPFTDLTLSGDSLRSEAELDPIMHPKCLPDFVSRYIGGGDVR
                     DPLASPVFGDYTGIPPLLIQVGEHEIIRDDSVRTAEKARAAGVDVTLEVWPGMFHVFQ
                     SHDPLLPEGREAIEHIADFTRTRRGA"
     gene            4880..5725
                     /locus_tag="PLT86_09840"
     CDS             4880..5725
                     /locus_tag="PLT86_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36952.1"
                     /translation="MATMVIGYLVEWTKDDVVTAQFLTQMRRIHEDARAPCTLYCVAD
                     VIARHREQLRDTGADPLFDVQIATKRPLKTVCQHAEGKTIVWPGASLEEIETEVAEAV
                     GAFVECFGSRPTGISGPLGAYRGLQDRPDVLAVLDRHGIRFSRTYARDQNDWQPVEFS
                     VEPFWYTYQGFSHIAEFPSQGWQEPIIRKIYGWDDAHGYLDYVKADVDDAVRRENLVW
                     SYWACDWSAIRGATDMAHIEGLIRYAQENGLELSTQKAAYERMVENETRAGNGDGSHE
                     ETPEP"
     gene            5779..7176
                     /locus_tag="PLT86_09845"
     CDS             5779..7176
                     /locus_tag="PLT86_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36953.1"
                     /translation="MGTQGLPRVTEIHHDPQLFVDDFLVDNRWADDHRAESVRRVFHQ
                     PVKHAANPVIAGDGGYVNVAHDDEAGLFRMWYQAYWDISYEPRKYTYAVAYAESPDGI
                     HWKLPRIRRHSFKNTLDNNIVFAGPFGDHASCQFIVDAPEEDRRGYRYVMLHGTRSAE
                     GHGMHLIGSHDGIEWDPASSSLIFPGYRPDTHSSILWDPRLRTYVCFTRATNLYDDTN
                     GLRRRVARLESPNLWDTWPIYPENVLIPDELDERSGHPLFYGMPTQYYAGIYWGFLWP
                     YAYGGDIHTELAFSRDGWNFQRLPERPRLVDRGPEGSWDAGMVFASTWLEVGDEWWLY
                     YSGTDGGHNSRENVPGIGLARVRKEGFVSLRSPAGGGTVVTKLMRWPGGRLLVNADAS
                     KSEFTVLATGYDRKPIDGFDPAPSLPVTGDRVRHEVVWAHGDIRALEGKPIRLEFLMR
                     GEVDLFGFQAVPEEA"
     gene            7179..8120
                     /locus_tag="PLT86_09850"
     CDS             7179..8120
                     /locus_tag="PLT86_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36954.1"
                     /translation="MGIAFSQVSDSAPWKPRYGLGVVVFAGRLWVLGGTASAANGTQF
                     NDVWSSEDGSEWRQELAAAPWSPRWNHATFAFGGKLWVIGGLASVDPLQNLDDIWSSP
                     DGRNWTLEVPDAPWAARHVWAWTIHRDRMFLIGGATDGSHTYRDVLSSEDGRRWRREA
                     VRDPWFCERKYHAAASCAGRLYVVAGVINDDSQPYGGRYLDDVWSSEDGAAWRCDASP
                     APWSGRAAPVLLACRDRLWLSGGELQPRLYGNDLWWTEDGVHWQREADEPPWPGRTIC
                     GSAVFRDKVWIMGGHHRDWPERVGHSLNDVWTCEVVP"
     gene            8368..>8747
                     /locus_tag="PLT86_09855"
     CDS             8368..>8747
                     /locus_tag="PLT86_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36955.1"
                     /translation="MDLEQHLEEIRAGLKAKQFTNEASVSQGIILRLLMALGWPTWDT
                     RIVAPEYPVKGGRVDYALCDPPEKPIAFVEVKGVGQSEGAERQLFEYAFHAGVPVVIL
                     SDGQLWHFFLPAEQGNYDERRVYKL"
BASE COUNT         1636 a         2571 c         2991 g         1549 t
ORIGIN      
        1 tcgtgtttcg cacgaactac gccacggtcg gcatctctaa ctgcttcgac ggcgagttcc
       61 cggagtccat ccgccggatg aggcttgtgg gtgcagaaat tctgctctgg tgcaatgcag
      121 cgtgcggaga ttcgcatttc ggcacaagcc accgcattca ccacagccag gcgcacgcgc
      181 aggccaacaa catgtgggtg gtgtgctgca actgcgtggc ggagaacgcc tcggggacaa
      241 gcctgatcgc gtcaccgcac gccgagccgc tggtgatcct gccgcctgac gaagaggcgc
      301 tcgccatcgc gacgatcgac cttgccgtgg tcacgtcgtg ggagccgtgg aaatcccgcg
      361 tggatgggag tggatacaag gagaaaccat gaaccaaccg aacgtgatac tcgtcatcac
      421 cgatgatcag ggctacggcg accttggctg cacgggcaac ccgtggattc agacgccgaa
      481 catcgaccgg ttctacgggg agtcgctccg cctgcgggat ttccacgtgt cgccgctctg
      541 cacgccgacg cgcggagcca tcatgacggg gcaccacccg gtgcgcaacg gggcgtgggc
      601 cacctgctgg gggcgttcga ttctgcggcg ggacgaggtc accatggcgg aggtgttcgc
      661 cgcgtcgggg taccgcaccg gcatgttcgg gaagtggcat ctcggcgaca accaccccta
      721 tcgccccatg gatcgcggat tccagcatgt cgtggcgcac aaaggcggcg gtgtggggca
      781 gactcccgat ttctggggca acaattactt cgatgacacg tatttccaca acggcgagcc
      841 cgtgccgcac gagggctact gcaccgacgt ctggtttgcc gaagcgacga agttcatcga
      901 ggcgaacgcc gatagcccgt ttttcgcgta catcgccacg aacgcgccac acgggccgtt
      961 cctcgtggcg gagaagtacc gccggatgta cgaggggaac ccggaaatcc cgaatgcggc
     1021 attttgcggg atggtgacga acatcgacga gaacttcggc aggctggagg cgaggctccg
     1081 ggaactgggc atagcggaca acacgattct gatcttcatg acggataacg gcagcgacgg
     1141 cgggctggtc atcggcgagg gcgaacgtat agtcaggggc tacaacgcag gcatgcgcgg
     1201 gaagaagggc tcgtattacg acggcgggca ccgcgtgccg tttttcgtgc gctggccgaa
     1261 cggcggcatc ggcgggggca aagacatcaa cgaaatggcg ttccatgtgg acctgctgcc
     1321 gacgttcatc agcctgtgcg gcctgaaagc tcccgagggc gtgaagttcg acgggatcag
     1381 ccttgccggc ctcatccacc gcagggagac cgcgctgccc gaccgcacgc agttcctgca
     1441 gtaccggcag gataccgtgc cacccgaaaa gtggacgaat gccgtggcga cgaagcagtg
     1501 gaggcttgtc cggggagtgg agctgtacga catcaaggcc gacccggggc aggagaacga
     1561 cgttgccgcg cggtaccccg aagtggtggc ccggctgcgc gaggcgcacg agcggtggtg
     1621 ggaggagatc agcccgcgcc tcgacgagta ctccccaatc agcctcggga acgacgccga
     1681 aaaccccact cggctcgacg ccatggacgt aatgggcgac atggcgttca gccagaacta
     1741 catcgtcctg gcgaagaaga gcaccgggaa gtggacggtg gacgtcgagc gggcaggcac
     1801 gtaccgtttc tctctccggc gctggccgaa ggagctcggg ctgccgatcg gggcggccgt
     1861 gtcgcccgaa gaggcgcgca gtctcacgtt ctcggatgga tcgggacgct gcacgacgat
     1921 tcgccctgcc cgcgcgcggc tgcggctgtt cgaccgggag tcagtgctgc ccgtggaccc
     1981 ggacgcgccg gaggtgacgt ttgaactcga ccttgcgcag accggggtga ctgtgctgga
     2041 ggcgtggttc gtggatgccg acggcgaaga gcgcggggcg tattacgtgt acgtggagag
     2101 ggtgtgaggg cggtcgtctc gatacaacga accggacagg aacgttgctt cgttccgttc
     2161 cttaccggtt cgccactcga cgaccggacg tttccgcgca acggcggaga gtgtagtggt
     2221 ctccgagtga cgcgccgtgc gccgggcgca cgtaagcgtt gtatcgagga gaccgttgac
     2281 gaggattctg gatgcctgcc ttcgcttcgt gctctgcgga gcacgcggaa atgacagctc
     2341 ctgttcgagg cggttgcttg gaaaacgacg agggacggcg accctgctca ggagcaacct
     2401 gacctttttc ggccaattgc cggcattccc gcgcaggcgg gaatccagcg gctcgggtcg
     2461 tctcgataca acctgcgctg gcgcgcaggc cactcgacga ccgttcttcg ttcgcgcaac
     2521 agcgagagga gcaactgcac ggtctccgag tgacgcggcg tgcgcagcgc gcacgtgagc
     2581 atcgcgtcga gaagaccatt gagggaaagc gccccatgac caatcgcgaa cgattcaacc
     2641 gcgtgatgcg gtacgaaccg gtagaccggc tgcccctgat gcggttcgag ccgcatcagg
     2701 tgtacgccgt ggagcgttgg cgcgccgaag ggttgccggc ggaggctgat ccggaccact
     2761 atttccgcat ctgccgcatc gttgacgccc cggtggacct gttcccgatc ccgcgtatcg
     2821 agcaggcgat cctgtctgaa accgacgccg attacgtgca ggtggaccgt ttcgggaacg
     2881 tgctccgccg gaaaaaggac tcgccgctga tgtactgcgg caacatcgcg cacccggtga
     2941 agaacatcgc ggattggagg gcatattccc ggcactacga cccgtacgcg cccggcagaa
     3001 tcccggaaga cctcgacgcc atcgcggcgg aactgaacgc atgcgacgcg ccggtgggaa
     3061 tttgcgtgtt tccctggttc ttccgcctcg gattctacgc gctggggatg gaaggctttc
     3121 tggcggcgtt ttacgagcag ccggcgctta ttcacgagat gttcgggttc tggagcgatc
     3181 tggtgctgca caccatccgg ccggtgctga aaaaggtgaa ggtggatgtt ctctacctca
     3241 cggaagacct cgcgtacaag aacgcgccac acgtgtcacc ccggatgtat gaacagttct
     3301 ggctgccgta ccagaacccg atcatcgagg aatgccgccg gcacggggtg gagcttgtat
     3361 gcctctggac ggcggggaac attgtccccc tgctgccgct ggcgatgcgg aacggcatca
     3421 actgcacgtg gccgctggag cgggtggccg gtatggaccc ggcgatgctg aggaagcggt
     3481 tcgggcggga gcttctcctt gcgggcggca tctcgaagga ggcgctggtc gccgggccgg
     3541 aggccattga tcgcgaaatc gagagcctga tgccggtgat acgcgacggg ggcttcatcc
     3601 cggcgctgga cgacatgccg cctgcggagg tcccgctggc tcattttgcg cacatgatag
     3661 atgcgctgcg gcggatagag gcttgaaagg tggggacgag ggtgtgcgaa tggtgacgtc
     3721 acaggagggg cgcgactact accagaagct gtgcgagacg ccatcgttcg gcgcgaacgg
     3781 atttgatttc gacggactgc gcaggggaat ggcgacgcgg cgcgagccga cgattccgga
     3841 tgtccagtgt atccgtatgg aaatcaaccg cataccgtgc gagtgggtca tggcgaagga
     3901 cgcggatccc gcggtgcggc tgctgtacct ccacggtggc ggattcattt cgggatccgg
     3961 ggcgttctat ctcccgctgg cagcgcatat ctctgcggcg gcaaagtgca tggtgctgct
     4021 gccggactac cgcctcgccc ccgagcaccc cttccccgcg gcgctggagg actgcctcag
     4081 catctaccag gcgctgatgg agatagagcc cgaggatgag gactcgagga cagcgacatt
     4141 catcgcggga gattccgcgg gcggcggcct gacgttgtcc accctgctgg cgcttcgcga
     4201 ccggggcatg gcgatgcccg acggcgggat tgcgctgtcg cccttcaccg acctgacgct
     4261 gagcggcgat tcgcttcgtt cggaggctga actggacccc atcatgcatc cgaagtgcct
     4321 gccggatttc gtgagccggt atatcggcgg gggagacgtg cgcgatccac tcgcgtcgcc
     4381 cgtgttcggc gactacaccg gaatcccgcc gctcctgatc caggtcgggg agcacgagat
     4441 catccgggat gacagcgtgc ggacggctga aaaggcgcgc gctgccggcg tggatgtgac
     4501 gcttgaagtc tggcccggca tgttccacgt gtttcagtcg cacgatccgt tgctgccgga
     4561 aggccgggag gcgatcgagc atattgccga tttcacgagg acgcggaggg gtgcgtgacg
     4621 acgggaacac gcccggggcg catggtcttc gagtggcgcg gggaacgcct cgcgttccac
     4681 gcgttgtatc gaggagacct gcaaggtggg cctcgattcc cgccttcgct tcgtgctcca
     4741 tcgggcacgc ggaacgacgg cctctttttg tctggaagga aacgtcattc tgagtgaagc
     4801 gagcacgagc gagcgaagtg aagaatcttc tgtttgtcac aaccgcaatg gcggaattcc
     4861 aggttaagga cacctatcaa tggcaaccat ggtgatcggg tatctggtcg agtggacgaa
     4921 ggatgacgtc gtcaccgcgc agtttctcac gcaaatgcgc cgcatacacg aggacgccag
     4981 ggcgccctgc acgctctact gcgtcgccga cgtcatcgcg cggcacaggg agcaactgcg
     5041 ggacaccggc gcagatccgc tgtttgacgt gcagatcgcc acgaagcggc cgctgaaaac
     5101 cgtctgccag cacgcggagg ggaaaaccat cgtctggccg ggggcgtcgc tggaggagat
     5161 cgagacggag gtcgcagaag ccgtcggtgc gttcgttgag tgcttcggca gccgtcccac
     5221 cggcatcagc ggtccgctcg gcgcatatcg tggtctgcag gatcgcccgg atgtgctggc
     5281 ggtgctcgac cggcacggca tccggttcag ccgaacgtat gcccgcgacc agaacgactg
     5341 gcagccggtg gagttctccg tggaaccgtt ctggtacacg taccagggct tctcgcacat
     5401 cgcggaattc cccagccagg gctggcagga gccgattatc cggaagatct acgggtggga
     5461 cgacgcacac ggctacctcg actacgtgaa ggcggatgtg gacgacgctg tccgccgtga
     5521 gaatctcgtc tggtcgtact gggcgtgcga ctggtccgcg atacgcgggg cgaccgacat
     5581 ggcgcacatt gaggggctga tccgctacgc acaggagaac gggctggagt tgagcacgca
     5641 gaaggcagcg tatgagcgga tggtggaaaa cgagacacgg gcaggaaacg gcgacggttc
     5701 ccacgaggaa acgccggagc cgtgagccgt gccgagacgc cctcaggccc gatacggccc
     5761 acaggaaagg cggcagatat gggaacgcag ggacttcccc gtgtgacgga gatacaccac
     5821 gatccccagc tctttgtgga tgatttcctt gtggacaacc ggtgggcgga cgaccaccgg
     5881 gcggagtctg tgcggcgggt gttccaccag cccgtgaagc acgcggcgaa tccagtcatc
     5941 gccggggacg gcgggtatgt caatgtcgcg cacgatgacg aagccggcct gttccgcatg
     6001 tggtaccagg cgtactggga tatcagctac gaaccgcgga agtacacgta tgccgtcgcc
     6061 tacgccgaat cgccggacgg catccactgg aagctcccgc gcatccggcg gcactccttc
     6121 aagaataccc tggacaacaa catcgtgttc gcgggtccct ttggcgacca cgcatcctgt
     6181 cagttcattg ttgacgcacc ggaggaagat cgccgagggt accgttacgt gatgctccac
     6241 ggcacgcgca gcgcggaagg ccacggcatg cacctcatcg gctcccacga cggcatcgag
     6301 tgggacccgg ccagcagctc gctcattttc ccgggctacc ggcccgatac gcactcgagc
     6361 atcctctggg acccccgcct gcgtacctac gtctgcttca cgcgcgccac gaacctctac
     6421 gatgacacga acgggttgcg gaggagagtg gcgaggctgg aaagtcccaa cctgtgggat
     6481 acgtggccga tctatcctga aaacgtcctg atccccgatg aactcgatga acgctcaggg
     6541 caccctctgt tctatggcat gccgacgcag tactacgccg gcatctactg gggcttcctc
     6601 tggccgtacg cgtacggcgg ggacatccac accgaactcg cgttcagccg cgacgggtgg
     6661 aacttccagc gactgcccga gcgccctcgt cttgtggatc gtggcccgga gggatcatgg
     6721 gatgccggca tggtgttcgc ctccacgtgg ctcgaggtgg gcgacgagtg gtggctgtat
     6781 tactccggga cagacggagg gcacaactcg cgggagaacg ttcccggcat cggcctcgcc
     6841 cgggttcgca aggagggatt tgtttctctg cgcagcccag ccggcggcgg tacggtggtg
     6901 acgaaactga tgcgctggcc gggcggcagg cttcttgtga atgccgatgc ctcaaaaagc
     6961 gagttcactg tgctggccac cgggtacgat cgaaaaccaa tcgacggatt cgacccggcg
     7021 ccgagccttc cggtcaccgg tgatcgtgtt cgccacgagg ttgtgtgggc tcacggtgac
     7081 atccgtgctc tcgaggggaa accgataagg cttgagtttt tgatgagggg agaggtcgac
     7141 ctgttcgggt tccaggcggt accagaggag gcctgagcgt gggaattgcc ttcagtcagg
     7201 tgtcggactc ggccccgtgg aagcctcggt acggcctcgg cgtggttgtg ttcgccggcc
     7261 ggttgtgggt gctcggcgga accgcttcgg ctgccaacgg gacgcagttc aacgatgtct
     7321 ggtcgtccga ggacggttcc gaatggcggc aggagcttgc ggctgcgccg tggagccccc
     7381 gatggaacca cgccacattc gccttcggcg ggaagctctg ggtgatcggc ggcctggcgt
     7441 cggtggatcc cctgcagaac ctggacgaca tctggtcgag cccggacggc aggaactgga
     7501 cgcttgaagt gccggatgcc ccgtgggcgg cccgtcatgt gtgggcatgg acgatccacc
     7561 gggaccggat gttcctcatc ggcggcgcga cggacgggtc gcacacgtac cgggatgtgc
     7621 tgtcctcgga agacggcagg cgttggcgcc gggaagcggt gcgggaccca tggttctgcg
     7681 agcgaaagta ccacgccgcc gcatcgtgtg ctggcaggct gtacgtggtt gcgggtgtca
     7741 taaatgacga ctcgcagccg tatggcgggc ggtaccttga cgacgtctgg tcatcggagg
     7801 acggcgccgc gtggaggtgt gacgccagcc ctgcgccttg gtcgggccgc gccgcaccgg
     7861 tgctgcttgc ctgccgggac aggctgtggt tatccggggg cgagctgcaa ccgaggttgt
     7921 acgggaatga tctctggtgg acggaggacg gcgttcactg gcagcgggag gcggacgaac
     7981 cgccgtggcc ggggcggacg atctgcggca gcgcggtgtt ccgtgacaaa gtgtggatca
     8041 tgggcggcca ccaccgcgat tggccggaac gggtgggcca cagcctgaac gacgtgtgga
     8101 cgtgtgaggt ggtcccgtag cggcgtgttt ggagtgcgca ggcaagccgg cgctttgaaa
     8161 gcggcggcag gccgccgcac tccaaaagaa cgcatcagat cacccaagtg aaaccctgtt
     8221 ggcaacctcg gacgtgtttg acgtacccgc accgagcctt tcctcgaagt agctttggac
     8281 cgacttgacc gagaccggca ttcctgttat caactagctc aaatcacggc acacaatcac
     8341 acgcgatttc gaatgagggc gaagcgcatg gatctggaac aacatctcga ggaaatccgc
     8401 gcaggcctga aagcgaagca gttcacgaac gaagcgtctg tttcacaagg catcatccta
     8461 cgccttctaa tggcacttgg gtggcctact tgggacacga gaatcgttgc tccggaatac
     8521 ccggtgaagg gcggccgggt ggactacgcg ttgtgtgacc cgcctgagaa gccgatcgct
     8581 ttcgtagaag tgaagggtgt tggtcaaagt gaaggcgccg aaaggcaact tttcgagtat
     8641 gcgttccacg ccggtgttcc tgtggtaata ctttccgacg gtcaactttg gcattttttc
     8701 cttccggcag aacaggggaa ctacgacgaa aggagagttt ataagct
//
LOCUS       DAOSOO010000135         8738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8019_length_8738_cov_4.962571, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000135 DAOSOO010000000
VERSION     DAOSOO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_8738_cov_4.962571"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="PLT86_09860"
     CDS             <1..1259
                     /locus_tag="PLT86_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOT36956.1"
                     /translation="CVEQTFRQPQIDDPAPIARAATLSLAAGAVKPGDRIAVPVGSRG
                     ISHIAGIVKAVVHALRDLCAQPFIIPAMGSHGGGTAEGQARVLETLGITEQTTGAPVV
                     SSMEVMPLGSTPEGIPVVIDANAARTDHVLLINRVKPHTEFGGRYQSGLAKMLLIGLG
                     KHQGAIRYHQAAVEMPFETLVFSAAQVVLERLPVLGGLAVLENAHGQVAEVVAVQADN
                     LLRDEPALLARATEYMATIPFDNIDLLIVDELGKDISGSGMDTNVIRRKHWADTRIGE
                     IVERPAPRRVFVRDLTEATEGNAAGIGLADFALTRVVNKVEWRKTRMNAITATRPRGA
                     MVPVLCENDAEAIEASLASAGVGDTVAARIIRIRNTACLNHMHVSDTLLPHAHAEASL
                     TYGEEPFALAFDEDGMLVPDKLPRGS"
     gene            1408..2331
                     /locus_tag="PLT86_09865"
     CDS             1408..2331
                     /locus_tag="PLT86_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT36957.1"
                     /translation="MRVLVTGGAGFIGSHIVDGLVERGHSVSVVDNLSSGRRENVNPA
                     ARLHVVDVTSNELGRVMEEERPEVVFHEAAQMDVRKSVADPRFDATVNILGGLNLYEE
                     CVRLKVRKVVFASTGGAIYGEQETYPAPETHPQQPLSPYGIAKLANEKYLYFYKSTYG
                     LEHVILRYANVYGPRQSPHGEAGVVAIFTSRLLSGQVPTINGTGEQTRDYVYVGDVAA
                     ANMAALDYGSSDVFNVGTGIETDVNELYALLRDAVGTSVEPIHGPGKPGEQMRSVVDW
                     QKAHRLLGWSPATPLPEGLKRTVEWFRQQRV"
     gene            2339..4102
                     /locus_tag="PLT86_09870"
     CDS             2339..4102
                     /locus_tag="PLT86_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOT36958.1"
                     /translation="MHGAIARTPQNRLFGVDTRGMRANALFLVVALAFSWGLGAARES
                     NTTALAPIFVTPRDPVYEFLDRAETKGLLSNAELSMKPWTRLSIARALLEINRADSSV
                     NRLTAVDKALLRFFAGEYRYEIRAISDGDPFKDLPSDPSQVFSDRVERWSRPSFEQDD
                     IDARVGIAVAYTARRDTGTLYRRHAEINGRLRKGSHWGFQAAFRDIAEWGKFSAPPPP
                     FDRTPGFAHVDGDTGRTYYYDDPEALISYCSGSVTASFGVFPLTIGRGRFGNVVLSDK
                     APAFPTVRLSFRPFDWLSLTYLHGSLQSGVPDTTSAWHGAAPSTAAFMSKYYVAHRIA
                     YTGIPGVEVGAGESLVYGGRGVAARYLIPVIPFRAAQHSEGDLDNLQMWGDLSVSRLP
                     WTKLYGSLFIDELSLNQLFQKDNIHNWWAWQYGAHVADFWGLIPDCDLFVEYTRANPW
                     VYRHRYPWNTYDTWSVRGNQPVTAYPLGFWQGHNGDYLRGEINWRPLRWLRVGAWVSQ
                     ARRGGDGSEIDQYNPPAEEFLFGPRTRTREIHVTGSVEWLKDLVLQCDVGRASRHADT
                     NAGRTDKSWTSISAGIAYGVW"
     gene            4142..5191
                     /locus_tag="PLT86_09875"
     CDS             4142..5191
                     /locus_tag="PLT86_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT36959.1"
                     /translation="MNIREPVHFLRKSLRWSKLWNVAQALSSYGLSRIVRRPIIWGMP
                     PVLMVEPTNVCNLHCPLCPSGNGTLRRKRGYMDFDLFRRAIDEISGKSLMVLLWNQGE
                     SFLHRDFLRMVRYASEHGLYTLASTNGHYLNSPEEIVRSGLDSLIISLDGATPETYTR
                     YRVGGDFDRVIHGIRQLAATKRRLRSKLPIIHVQFILFRHNLHELEKIRELAASLGAD
                     KVTYKTAQVYDGKEAEDFLPREPRFRRYQSVNGEVVSKAAARGSIPNRCRTLWVQPVL
                     NWDGTVTPCCFDKHGDFAVGYLSEETTLRRIWHGELMNAFRRRVMTDRGALAMCRNCT
                     EGIKTNYREEDIRIV"
     gene            complement(5256..6335)
                     /locus_tag="PLT86_09880"
     CDS             complement(5256..6335)
                     /locus_tag="PLT86_09880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT36960.1"
                     /translation="MNRGFCLRGAVCAALFAASSGVAQTTQYIKDGVYDPATPERRGQ
                     AGATFLQVGASARAEGMGGAFAGVRGDPAIAFYNPAGVAEVTGVAFSADHTKWVADMS
                     LNGLVVAARARFVSLGVSFLSMDYGSFDGTRIADNAAGYESIGAVTPTAWALGGIAAV
                     KFTDRFNAGIQTKWLVQDIGESEVYTFRNPPRYTGEMTKNRVSTWAIDLGTQYYTGLR
                     NLSINMTLQNFGGTQKYVETTFSLPLTYRVGVSAELMEFVTGDATGAHKVFLSMEGVD
                     RRNVSLDGAIGAEYTGDMSDFVPGLSVSVRAGRRPARNQDGWMSFGGGVCFPVTGVKM
                     GLDYSFNDYGPHLSVHRVGMRASLR"
     gene            complement(6376..6774)
                     /locus_tag="PLT86_09885"
     CDS             complement(6376..6774)
                     /locus_tag="PLT86_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36961.1"
                     /translation="MALFSRRWALPTAILVAFLCVFASDAISQTTTLDSVKVVPNPYN
                     VSGRTYASRSNIFGYERLRFANLPLPCTVKIYSSDANLVKTLQFNAGDLLPAWDGRNS
                     DNQYIVSDVYFYVVEHATLGTKIGKFVVIR"
     gene            complement(6836..>8738)
                     /locus_tag="PLT86_09890"
     CDS             complement(6836..>8738)
                     /locus_tag="PLT86_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36962.1"
                     /translation="ATGDTLIDFIFFPGSAGLDQFKYPASGGKGPRPDVIVDPNLVTE
                     LKINTVWRYIMGVESIRDVYGYGQTSPHQDYVFTDYKLVNNGISGRTANDHPPSDGAT
                     IQNQVINKMLWWHTRHLKDEMASVANQQCGDQEMMYVEPFGAGNHSIMMAWDVDLDDP
                     TSPGPDWGDPIETAFFEGHMAGNAYIVNGPVFASAGPGANYGVDDPAQPAFRTVWQEY
                     GFDFGKTYSPADPAQAREMIADGSLQMDLNVGFRDDPRFVNYVGGANIASAIWGYGKL
                     AGPLNLANVRSQGYDMPWLDSLRFVEVMGAGGIDVEEARRIGNAWIANKAANPGNPAS
                     WMSAADIALVQTGRDTAMKACALSYWNFHGHMPANVTAADLAKWGLTGFVTSKPAQYG
                     PFDAPDAPRPPGFLSVRPLNRVGIEVRWGKEAETTPDHDTKVMDFAGYRIYRQTGTRM
                     SPMVLIKQGPASDFAEVAADGAFPAGRVFHDVNVTPGVDYWYAVVAYDDGTQNWELPG
                     YSMESTRWWTWSGYMNKGATAPEYVGVDASAAPGKFMLAQNTPNPFNPTTTIKFSLAS
                     SAQTKLVVYSLTGQVVRTLINGQTAAGVHSVVWDGNDNMGRPVASGVYLYRLTSGTNE
                     MVRRMVLVR"
BASE COUNT         1825 a         2680 c         2517 g         1716 t
ORIGIN      
        1 ggtgcgttga acagacgttc cgccagccac agatcgacga tccagcgccc atcgccagag
       61 cagccacact gtcacttgct gccggcgccg tgaaaccggg tgacagaatc gcggttccgg
      121 tgggcagcag gggcatttcg catatcgcag ggatcgtgaa ggcggtagtc cacgcgcttc
      181 gcgacttgtg cgcgcagccc ttcatcatac ccgcgatggg cagccacggc ggggggaccg
      241 cggaaggcca agcgcgggtc ctggaaacgc ttggaatcac cgagcagaca acaggggcac
      301 ccgtggtatc ctccatggag gtcatgccgc tgggttccac gcccgaaggt atcccggtcg
      361 tcatcgacgc gaacgcggca cggacagacc acgttctgct catcaacagg gtcaagcccc
      421 acacggaatt cggaggtcgg taccagagcg gccttgcaaa gatgctcctc ataggtcttg
      481 gaaagcacca gggagccatc cggtaccatc aagccgccgt ggagatgccg tttgaaacct
      541 tagtcttttc agcagctcaa gtcgtgctcg agcgcttgcc tgttctcgga ggtcttgccg
      601 tgcttgaaaa cgcacacggg caggtcgctg aggttgtggc ggtacaggct gacaatcttt
      661 tgcgcgatga gccagccctt ctggccagag cgacggagta catggcaacg atcccgtttg
      721 acaacatcga tttgctgatt gtggatgagc tgggaaagga tatcagcggg tccgggatgg
      781 acaccaacgt cattcggcgc aaacactggg cggatacccg catcggggag atagtagagc
      841 ggccagcgcc ccgtcgtgtg ttcgttcgtg atctgacgga ggcaaccgag gggaacgccg
      901 cgggcatcgg ccttgcagac ttcgcactta cgcgcgtcgt caacaaagtg gagtggcgca
      961 aaacgcgcat gaacgccatt acggcaacgc gcccgcgtgg agcaatggtg cctgtcctgt
     1021 gtgaaaacga cgcggaagca atcgaagcct ccctggcctc cgccggagtt ggagacactg
     1081 ttgccgcgcg tatcatccga atacggaaca ccgcgtgcct gaaccacatg catgtcagcg
     1141 acactctcct gccacatgct catgccgaag cgtctctcac gtatggggaa gagccattcg
     1201 cgcttgcgtt cgatgaggac ggcatgttgg tgccggacaa acttccacga gggtcttgat
     1261 ccaattccga cttccgcgcg cagaaaagtg ctgcagagac catccagcca atgcatgcaa
     1321 tcggacaatt cgttactttc gaaaatgcaa gaggataccc gcccgggtca tttggcattt
     1381 caccgtattc attctgtgga gaacatcatg cgcgtactgg taaccggtgg ggccggtttc
     1441 atcggttctc acatcgtgga cggcctcgtc gaacggggac actcggtcag cgtcgtggat
     1501 aacctttcgt cagggagaag ggagaacgtc aatccggctg cacgacttca cgtcgtcgat
     1561 gtgacatcga acgaactggg gagggttatg gaggaggagc gccccgaagt ggtgttccac
     1621 gaggcagccc agatggacgt tcgcaagtcc gtggccgatc cgcgtttcga tgccacggtg
     1681 aacatcctcg ggggcctcaa tctgtacgag gaatgcgtcc gattgaaggt tcgaaaggtg
     1741 gtgtttgctt ctacaggcgg cgcaatttac ggcgaacagg agacgtaccc cgcgcccgag
     1801 acacacccgc aacaaccgct ttcgccgtac ggaatagcca aactcgccaa tgagaaatac
     1861 ctgtactttt acaaaagcac gtacgggctg gaacacgtca tcctcagata cgcgaacgtg
     1921 tacggaccgc gacagagccc ccatggcgag gccggggtcg tcgcgatatt cacgtcgcgt
     1981 cttctttcgg ggcaggttcc gacaatcaac ggaactggag aacagacgcg agattacgtg
     2041 tacgtgggag acgttgccgc tgcgaacatg gctgcactcg attacggcag cagcgacgtg
     2101 ttcaacgtcg gaacgggcat cgaaaccgat gtgaatgagc tctacgcgct cctccgcgac
     2161 gctgttggca ccagcgtgga gccgatccat ggtccgggca aaccgggtga gcagatgcgg
     2221 agcgtggtgg actggcagaa agcgcaccgt ctcctcggat ggtcgccggc gactcctctc
     2281 ccagaaggtt tgaaacgaac cgttgaatgg tttcgccagc aacgcgtata ggtgttgaat
     2341 gcacggcgca attgcccgaa cccctcagaa ccggttgttc ggtgttgaca cacgcggaat
     2401 gcgagcgaac gcgctgtttc tcgtggtggc acttgcgttt tcatggggac ttggggccgc
     2461 acgcgagagt aacacgacgg ccctggcacc catcttcgtc acgccgcgtg accccgtgta
     2521 cgaattcctt gaccgcgcgg agacaaaggg cctcctgagt aacgctgagc tttcgatgaa
     2581 gccctggacc cgcctttcaa tcgcacgggc gctccttgaa atcaaccgcg ccgattcttc
     2641 tgtgaatcgt ctcactgcag tggacaaggc gctcctgcgc tttttcgcag gcgagtaccg
     2701 gtacgagatc cgcgccattt cggacggcga tccgttcaag gacctgccgt cggacccttc
     2761 tcaggttttc tccgaccgtg tggagcgctg gtcacggcca tcatttgaac aagacgacat
     2821 cgacgcccgc gtcgggattg ccgtcgcgta caccgcccgg agggacaccg gtacgttgta
     2881 ccggcgacac gcggagatca acgggcgtct gcggaagggg tctcactggg gtttccaggc
     2941 tgcattccgc gatatcgccg aatggggaaa attcagcgca ccgcctcctc cgttcgatcg
     3001 aacgccggga tttgcgcatg ttgacggcga tacaggccgc acatactact atgacgatcc
     3061 tgaagctctc atttcctatt gctccggctc tgtcaccgcg tcgttcggag tattcccgct
     3121 cacgatcggg cggggtcgct tcggcaacgt cgttctgtcc gacaaagcac ccgcgttccc
     3181 aaccgtgcgc ctgtcgttcc ggccctttga ctggctttcg ctgacgtacc ttcacggatc
     3241 gttgcagagc ggcgtaccgg ataccaccag cgcgtggcac ggcgccgccc cttccactgc
     3301 tgcgttcatg agcaaatact acgtcgccca cagaatcgcg tacaccggta tccccggcgt
     3361 cgaggtcggc gcgggcgaat ccttggtgta cgggggccgc ggggttgccg cccgatatct
     3421 tatccctgtg ataccttttc gcgcagctca acacagcgaa ggcgacctcg acaatctgca
     3481 gatgtgggga gatctgagcg tgtcgcggtt gccctggacg aagctgtacg ggtccctgtt
     3541 catagacgag ttgagcctca accagctctt ccaaaaagac aatattcaca attggtgggc
     3601 ctggcagtac ggagcgcatg tggcagattt ctggggactc atccccgact gcgacctttt
     3661 cgtcgagtac accagagcaa acccctgggt ataccggcat cgctacccct ggaacacgta
     3721 tgacacgtgg tccgtgcggg gaaaccagcc ggtgacggcg tatccacttg gtttctggca
     3781 gggccacaac ggggattacc tccggggaga gatcaactgg cgcccgctgc gctggttgcg
     3841 tgtcggcgcg tgggtgtctc aggcgcgacg aggtggtgac gggagcgaaa ttgatcagta
     3901 caacccgccg gcggaagagt tcctttttgg tcctcgcaca cgaacgcgtg agatccatgt
     3961 taccggtagc gtggaatggc tcaaggatct tgttctgcag tgcgacgtgg gcagggcatc
     4021 tcgccacgcg gataccaacg cagggcgcac cgacaagagc tggacgagca tctccgcggg
     4081 cattgcatac ggcgtatggt gacgtgacaa aacccctcac acccggattc ctgcaaatcc
     4141 tatgaacatt cgcgaacccg ttcatttcct tcgcaagtcg ttgcgctgga gcaagctttg
     4201 gaatgtcgcg caggcacttt caagctacgg gttatcgcga attgtcaggc gtccgatcat
     4261 ctggggcatg cctccggttc tgatggtaga gcccacaaac gtgtgcaatc tccactgtcc
     4321 cctttgtccc agcggcaacg gaacgctgcg cagaaaacgc ggctacatgg actttgacct
     4381 tttccgccgc gccatagacg aaatatccgg caaaagcctt atggtgctcc tgtggaacca
     4441 gggagagtcg ttcctgcacc gcgactttct ccgaatggta cggtacgcct cggagcacgg
     4501 cctgtacaca cttgccagca caaacggcca ttacctcaac agccctgagg aaattgttcg
     4561 gtccggcctg gacagtctca tcatcagcct cgacggagcg acgcccgaaa cgtacaccag
     4621 ataccgggtc ggcggcgatt tcgaccgggt catccacggc attcggcaac ttgccgccac
     4681 aaaacggcgc ctacgatcga aattgcccat tatccacgtc caattcatcc tttttcgaca
     4741 taatcttcac gagctagaaa agatacgcga actggccgcg tctctcggcg cggacaaagt
     4801 gacgtacaaa actgctcagg tctacgacgg gaaagaggcc gaggacttct tgccgcggga
     4861 gccgcggttc cgacgctacc agtcggtcaa tggagaggtc gtgagcaaag cagccgcaag
     4921 aggaagcatc ccgaaccggt gcaggactct gtgggtgcaa ccggtcctga actgggacgg
     4981 aaccgttacc ccctgttgtt tcgacaaaca cggagacttc gcggttggat acctttcaga
     5041 ggaaacaaca ctgcgccgca tctggcacgg agaactcatg aacgcgtttc gaaggcgcgt
     5101 tatgaccgac aggggagcat tggctatgtg caggaactgc acggagggaa taaaaacaaa
     5161 ttatcgcgaa gaggacatcc gcatcgtctg acggaaggcc ggatcactca cctctccggc
     5221 ctgcacacta cccgcacttc cttcgccaga tcggttcaac ggagcgaagc ccgcatccca
     5281 acgcggtgga ccgaaagatg cggcccgtaa tcgttgaacg aatagtccag ccccattttg
     5341 actccggtca cgggaaagca caccccgccg ccgaagctca tccaaccgtc ctgattccgc
     5401 gcaggccttc ttcccgccct cactgatacc gaaaggcccg gaacaaagtc ggacatgtcg
     5461 ccggtgtact ccgcgccgat cgcaccgtca aggctcacat ttcgacgatc gacgccctcc
     5521 atcgacagga acaccttgtg cgcgcccgtc gcgtccccgg tgacgaactc catgagttcc
     5581 gcagacacgc ccacgcggta cgtgagcgga aggctgaacg ttgtttctac gtacttctgc
     5641 gtcccgccga aattctgcag tgtcatgttg atactgaggt tgcggaggcc ggtgtaatac
     5701 tgggtgccga ggtcgatcgc ccaggtgctc acccggttct tggtcatttc gccggtatac
     5761 cgcggcgggt tgcggaatgt gtagacctcg ctctcgccga tgtcctgcac cagccacttc
     5821 gtctgaattc cggcattgaa ccggtcggtg aatttcaccg ccgcaatacc gcccagcgcc
     5881 catgcggtcg gtgtcaccgc gccgatggat tcgtacccgg cggcgttgtc tgcgatgcgc
     5941 gtgccgtcaa aagacccata atccatgctc aggaacgaga cgccaagtga gacaaaccgt
     6001 gcacgcgccg ccaccaccag gccattcagt gacatatcgg caacccactt cgtgtggtcc
     6061 gccgaaaacg ccacccccgt tacctccgcc acgccggcag gattgtagaa tgcaatcgcg
     6121 ggatcccccc gaacacccgc aaaggctccc cccatgcctt ccgcgcgtgc gcttgccccc
     6181 acctggagga acgtcgctcc tgcctgtccc cggcgctccg gtgtcgccgg atcatataca
     6241 ccatctttga tgtattgagt ggtttgagcc acaccggacg aagccgcaaa cagggccgca
     6301 cacacggcgc cacgcaggca aaatcctctg ttcatggtat ctcccgagat cgacatcctg
     6361 agcaactgcc gcacgtcaac gaattacgac gaacttgccg attttcgtcc ccaaggttgc
     6421 atgttcaacc acatagaaat acacgtcgct gactatgtac tggttgtcgc tgttacgccc
     6481 atcccacgcc ggcaacagat cgcccgcgtt gaactgcagc gttttcacca gattcgcatc
     6541 cgacgaataa atcttgaccg tacacggcaa cgggagattc gcgaaccgca gccgctcgta
     6601 cccgaaaata ttcgaccgag acgcatacgt tcttcccgaa acgttgtacg ggttgggaac
     6661 tactttcacg ctgtccagag tggtcgtctg cgaaatggcg tccgacgcga acacacagag
     6721 gaacgcgacc aaaatggctg tcggcagagc ccatcgacgg gaaaatagag ccattgccca
     6781 tccctttcag acaggcgtgg gaggcaccaa cctcccacgc ccgccgaatc gctgcttaac
     6841 gaacaagtac catgcggcga accatttcgt tcgtgccgct ggtcaagcga tagaggtaca
     6901 ctccacttgc aaccggacga cccatgttgt cgttgccgtc ccacacaacg ctgtggacac
     6961 cggcagcggt ctgcccattg atcagcgtgc gaaccacctg accggtaagg ctgtagacaa
     7021 caagtttcgt ctgggcggaa cttgcaaggc tgaacttgat ggtcgtagtc gggttgaacg
     7081 ggttcggggt gttctgggcg agcatgaact tgcccggagc tgcagatgcg tccacgccta
     7141 catattccgg cgcggtagca cccttgttca tgtacccgga ccacgtccac cagcgcgtgc
     7201 tctccatcga gtacccgggg agctcccagt tctgggtgcc gtcgtcatac gcgacgaccg
     7261 cgtaccagta gtccacaccg ggggtcacgt tgacatcgtg gaacacacga cccgccggga
     7321 acgcaccatc ggcagccact tccgcgaagt cggacgcagg cccctgtttg atcagcacca
     7381 tcggagacat acgcgtcccc gtctggcgat agatccggta ccccgcaaag tccatgactt
     7441 tggtatcgtg gtcgggagtt gtttctgctt ccttacccca gcggacttcg attcccacgc
     7501 ggttcaacgg acgcaccgag aggaatccgg gcggacgagg agcatccgga gcatcgaacg
     7561 gcccgtattg cgccggcttc gacgtaacaa accccgtcaa cccccacttc gccaggtcag
     7621 ccgcagtcac gttggcgggc atgtggccgt gaaagttcca gtacgaaagc gcgcaggctt
     7681 tcatcgccgt atcgcgtccc gtctgcacga gcgcgatatc cgcggccgac atccagctcg
     7741 ccgggtttcc cgggtttgcg gccttgtttg cgatccacgc gttaccaatg cgccgcgctt
     7801 cttcaacgtc aataccaccc gcacccatca cttcgacaaa ccgcagggaa tcgagccacg
     7861 gcatgtcata cccctgagaa cgcacattcg cgaggttcag cggccccgcc aatttgccat
     7921 aaccccagat tgcggaggca atgttcgcgc cgccaacgta attgacgaag cgaggatcgt
     7981 cccggaaacc gacgttcaag tccatctgga gagaaccgtc ggcaatcatt tccctggcct
     8041 gcgccggatc tgccggggag taggtcttgc cgaagtcaaa cccgtattcc tgccagacgg
     8101 tgcggaacgc gggctgtgcc gggtcgtcaa cgccgtaatt ggcgccgggc cccgcgctcg
     8161 cgaacaccgg accattcacg atgtacgcgt taccggccat gtggccttca aagaacgcgg
     8221 tctcgatcgg gtcaccccag tcgggtccgg gtgaagtcgg gtcgtcgagg tcaacgtccc
     8281 acgccatcat aatgctatgg ttgcccgcgc cgaacggttc gacatacatc atttcctggt
     8341 cgccgcactg ctggttggca acgctggcca tctcgtcctt cagatgccgt gtgtgccacc
     8401 agagcatctt gttgatgacc tggttctgga tggttgcacc gtcggatggg gggtgatcgt
     8461 tcgccgtacg gccgctgata ccattgttta ccagtttgta gtcggtgaag acgtagtcct
     8521 ggtgcgggct cgtctggcca tacccgtaga cgtcacgaat gctttcgaca cccatgatgt
     8581 accgccatac ggtgttgatc ttcaattccg tgacgagatt cggatcgacg atcacatcag
     8641 gacgtgggcc tttcccgccg ctcgccggat acttgaactg gtccagcccg gcgcttccgg
     8701 ggaagaagat gaaatcaatc agcgtgtcgc ccgttgcc
//
LOCUS       DAOSOO010000136         8719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8047_length_8719_cov_5.730148, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000136 DAOSOO010000000
VERSION     DAOSOO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8047_length_8719_cov_5.730148"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..896
                     /gene="tsaB"
                     /locus_tag="PLT86_09895"
     CDS             222..896
                     /gene="tsaB"
                     /locus_tag="PLT86_09895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT36963.1"
                     /translation="MTVLGIDSATPGLGVALAGPEGLIAETTIHVGLQHSQTLLPAAE
                     WLLETVGAGTRDIEGVAVTIGPGSFTAVRIGLATAKGICMAGGIPLACVSTLAASAAR
                     HAWVSLPVVPMLDAKRREVYVGLYDVSTGEPVAVEPDTVVSPQDWLEGHKGEALFCGD
                     GALAYRDLITEKLGAKAHFTPSWLPLASAGVVAVLGRQKILKGETIQPDAATPVYLRR
                     PQAVLP"
     gene            949..1407
                     /gene="rimI"
                     /locus_tag="PLT86_09900"
     CDS             949..1407
                     /gene="rimI"
                     /locus_tag="PLT86_09900"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT36964.1"
                     /translation="MSPIIRAVAADDVEDIAHLETICFPDPWSRASISRILETPSSAI
                     CVAEKEPQGILGYAITTSVSGEGELERIGVAPACRRTGVGRSLLRYALGQLVQAGVKV
                     VWLEVRRSNESALRLYEREGFELVRVRKGYYPPREDALIMRKLLVPEPAG"
     gene            complement(1432..2169)
                     /locus_tag="PLT86_09905"
     CDS             complement(1432..2169)
                     /locus_tag="PLT86_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT36965.1"
                     /translation="MSECAVLLAAGPGTRLRPLTDTIPKCLVPVGGRPMLDWWLELLA
                     RHGVDRALVATNYLSDQVKAFVRRQKHAVEVEVRDEPVLLGTAGAVTAQAEWLEKEES
                     FWVIYVDNLSSADLSAMRSFHREKKGIGTLGVYETPVPSQCGIVELKNGEEANGSGQV
                     LRLVEKPAFPAGNLANGGLYLLDREILDHIPAPPCDFARDVFPAILAQPSSRPLFAWR
                     IRGYHLDMGTLETYQRAEQDVRNGVFP"
     gene            complement(2162..3271)
                     /locus_tag="PLT86_09910"
     CDS             complement(2162..3271)
                     /locus_tag="PLT86_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36966.1"
                     /translation="MNVTSETRLCLTSTLERCSVTATAPCRVDASGTWDLKALALPYE
                     HIRPSTANIALNMRTSARLEPYKPGWVLVRNDETYETFPADEVPLDSRFGLVFAAVSH
                     CAVDGVSLTLAHEAPRYAGLGGSGTLMVAVLGALKAAIAVARGERGATLSDEQRAELT
                     ALAHHIEDGLHFSNTGMQDQAAAAFGGVHQWEWRYAANPPFARRSLVPPGEEHHLSDR
                     MAIAYVGASHDSSDVNAAQIRDFFSGTTRAQWLEINALGRAFADAIRRGEYGVAADAL
                     QREHEIRCSLVPRRITPVGRVLEGIARDFGCGFAVSGAGNGGCVWALGTDPACVQKLR
                     DAWAATLQEVPTGKVLSSEIAMEGLIVDAGRSNDE"
     gene            complement(3293..4069)
                     /locus_tag="PLT86_09915"
     CDS             complement(3293..4069)
                     /locus_tag="PLT86_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT36967.1"
                     /translation="MRGLITVLVLAISLGVAGCRGKPDLAAVYVQQGRWGEAARLYRE
                     HLAQHPDDQLALRSLAALECYRLGQLEECARTAEHLLSLMPIDSDGVAAVAYARTLLA
                     ERATRQNDTARVRVEMMKVGEAYFKAGYWEYMRENFQRSERFLRKAVLVDPNRVDAYL
                     RLGILFWNRHQTDSSLAWFARAERVAPMNEDALVNQIVVLWQANRIEEAKAVLERLNL
                     VRAQLYPDSSFASPVDTSLPPLSLDFRGDVIEQKKHLITF"
     gene            complement(4066..4884)
                     /locus_tag="PLT86_09920"
     CDS             complement(4066..4884)
                     /locus_tag="PLT86_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT36968.1"
                     /translation="MEKVHSEETPTRIWVGTSGFHFDDWQGVFYPSGLPRSRWLDYYV
                     KSFRVLELNSSYYRIPSADSMQRLAEKMPETHGIVVKANRLTTHEYKDEDASGPFRSA
                     LEPLREKWLLRGVLAQFPWSFRNTPGNFQYLAEVTGRMNDVQWFVEFRHRSWLVPEVG
                     AFLRENAIGFVSVDEPQLREMMPPVAKFTVPTAYIRFHGRNADSWWAGGGADRYDYRY
                     SDEELKEWVGKVARLSGQVREILIFFNNCKGGSAAINAKAMRELLKTLPGVEVQ"
     gene            5148..5825
                     /locus_tag="PLT86_09925"
     CDS             5148..5825
                     /locus_tag="PLT86_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT36969.1"
                     /translation="MKTALTRSIVLVAVTATTLVLGGCGLSRAVKGGAIGAATGGAIG
                     AAIGNRMGSTATGAIAGAAIGGTAGAVIGHYMDRQAEEMAANLDSASVERVGEGIKLT
                     FGSGILFRSGSADLTPEASANLVQLADILKKYPDTNLIIEGHTDSDGSESFNQTLSER
                     RANTVRTALVNNGVAAERLTAVGYGESQPVMANDTPAGKAANRRVEIAVFANDKLKQE
                     AQASAAQ"
     gene            6012..6770
                     /locus_tag="PLT86_09930"
     CDS             6012..6770
                     /locus_tag="PLT86_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT36970.1"
                     /translation="MKNSLPKGTTLIACIGIALVTGCANLTWCGLKTAERIEPAVERV
                     LPPAPIVAPVPTAPAEPVVVPAPATVVEAPPAPPPPPPPPPVVSVPPVVPAAPPPTEV
                     EVYMERQADEITTDLRSVATVQRTGEQIKITFGSGVLFGPGSAALTAQAQESIAKLAA
                     IIKKYPDTNLRVEGHTDSDGAEASNQRLSERRANAVAEALRSNGIPAARVTAIGYGET
                     RPVGSNDTPEGKAANRRVEITVTGGAILREGSSR"
     gene            7058..7624
                     /locus_tag="PLT86_09935"
     CDS             7058..7624
                     /locus_tag="PLT86_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT36971.1"
                     /translation="MRNASARVGWVFAAALSVIAAGCCGLNTAPGTARPASDATAVNA
                     YMDQQVGEMRGDLENAQVQREGQRIKVTFGSGVLFGTGSAELTPAARENISRLAGILK
                     RYPNTNVSVEGHTDSDGAEDFNQALSERRANAVAQALRDEGVAGSRIRTVGFGETQPI
                     ATNDTPEGKAANRRVEIIVMANESFGGR"
     gene            7706..>8719
                     /locus_tag="PLT86_09940"
     CDS             7706..>8719
                     /locus_tag="PLT86_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT36972.1"
                     /translation="MIYRIVAPEAGEILVSPKTGSVSLAYNGNPIDYFDRDGRLLGSQ
                     VGDRHYRRGLDNRLLEKYRPPEEGEHVRNEVPEPARQRLITAAYERAGMLADAVSRSS
                     IECRPTHPSDMALPLSAREALAGFLNPILAQGFERLCVEAMEFQRMYAPVSILPPDQY
                     LSVVAQLTTGCSYNRCTFCNFYKSRGFTVKSADEFRRHLSEVVSFPATFARLRKSVFL
                     ADGNALSLPHTKLLEMFGVLHEFFSFVPSQVCEIRERYAYRKSHPHSYEGVFSFVDGR
                     AAMEKTVDDFRELKEQHLRRVYVGLESGDDALLTFVRKPATRSKIIDAVRRMKTAGLN
                     VGVI"
BASE COUNT         1810 a         2569 c         2615 g         1725 t
ORIGIN      
        1 aagtccccag aatgcgcatc ggtggcgcag aagggaaaga aaaggccaaa ataaggggtg
       61 gaacaatcgc gaacacatgc ttttgaaacc acataacccg tgttcaaaaa taaaggattc
      121 tgcgggaacc cgcaagcaga tgcgttacac atgctggcaa acgacagaaa tcttctggga
      181 tatatcgcgc ggactgcaaa acggaaggat agagagggta tatgacagtt ctcggcatcg
      241 actcggcgac accaggtctc ggagtggcac tggccgggcc ggaagggctt atcgcggaaa
      301 caaccatcca tgtcggactc cagcactcac agacactcct gcctgcggca gaatggttgc
      361 tcgagactgt gggggcgggc acccgtgata tcgagggtgt ggcagtgacg atcggccccg
      421 gctcttttac cgctgtgcgg atcggccttg ctacggcaaa gggtatctgc atggcgggcg
      481 gaataccgct tgcgtgcgtt tccaccctag ccgcgtccgc agcgcggcat gcctgggtaa
      541 gtctgcccgt tgttccgatg ctggatgcca agcgtcgcga agtgtacgtc gggctctacg
      601 atgtgtctac gggagaaccg gttgcggtcg aaccggatac tgtggtgtcc ccccaagact
      661 ggctggaagg ccacaagggg gaggcactgt tctgcggtga cggcgcgctg gcataccgtg
      721 atctaattac cgaaaaactt ggcgcgaagg cacatttcac gccgtcgtgg ttaccgcttg
      781 cttccgccgg agtggtggct gttctgggac gacaaaagat actgaagggc gagaccatcc
      841 agccggatgc ggccacacct gtgtatctgc ggcgtcccca agccgtcctc ccgtaacgca
      901 gccgggacct gcggtgcgaa ttcctaaccg ttgcgagcga gcgcgagaat gtcaccaatt
      961 attcgggccg tggctgctga tgatgttgaa gacatcgccc atctggagac aatctgtttc
     1021 cctgatccct ggtcaagagc cagcatttcg aggatcttgg agaccccgtc gagcgcgatc
     1081 tgtgttgccg agaaagagcc gcaaggtatc ctggggtacg cgataacaac cagcgtatcg
     1141 ggcgaaggcg agctcgagcg catcggggtt gcgccagctt gcagacgcac cggcgtgggt
     1201 agatcacttc tccgctacgc tctgggccaa cttgttcagg caggcgtgaa agtcgtgtgg
     1261 ctggaggtga ggcgttccaa cgaaagcgcg ttgaggttgt acgaacgaga agggtttgaa
     1321 ctggtacggg ttcgcaaggg gtattacccg ccccgtgaag atgcgctcat catgaggaag
     1381 cttcttgttc ctgaacccgc cgggtgatga tgccggaacg cgcaccggag cctacggaaa
     1441 cacgccgttt cttacgtcct gttctgctcg ttggtacgtc tccagcgttc ccatgtcgag
     1501 gtgatatcct cggatccgcc acgcaaaaag aggcctcgaa gaaggttgcg cgaggatcgc
     1561 agggaacaca tccctcgcaa aatcgcaggg tggcgcaggg atatggtcca ggatttcgcg
     1621 gtcgagcagg tacagaccac cgtttgcaag gttccccgcg ggaaaggccg gtttctctac
     1681 caggcgcaga acctgaccgg atccatttgc ttcttcgccg ttcttcagtt ccacgatgcc
     1741 gcactgggag ggaaccggtg tttcatacac gcccagagtt ccgatgccct tcttctcccg
     1801 gtgaaacgac ctcatggcgg aaagatcggc gctggagagg ttgtccacgt aaatgaccca
     1861 gaacgactcc tccttttcca accattccgc ctgcgccgta actgcaccgg cggttccgag
     1921 aagaaccggc tcatcgcgca cctccacctc aacggcgtgt ttctgccggc ggacgaacgc
     1981 tttcacctgg tccgacaaat agttggttgc gacgagagcg cgatcaacgc cgtgccgcgc
     2041 gagaagttcc agccaccagt ccagcatcgg tctcccgccg accgggacga gacacttcgg
     2101 aatcgtgtcg gtcaacggac ggaggcgggt gcccggcccc gccgcaagca ggacggcgca
     2161 ttcactcatc gtttgacctg cctgcgtcca caataagccc ttccattgct atttcggagg
     2221 aaagcacttt cccggtaggc acctcctgga gtgtggctgc ccacgcgtcc cgcagcttct
     2281 gaacgcacgc tgggtccgtt cccaacgccc agacacaacc gccattgccg gcgcccgaca
     2341 ccgcaaagcc gcatccgaaa tcgcgagcga taccttccag gacgcgtccg accggtgtga
     2401 tgcggcgagg cacgaggctg caccgaatct cgtgttcccg ctggagtgcg tccgccgcaa
     2461 cgccgtactc accccggcgt atggcgtcag cgaaagctct ccccagcgcg ttgatttcaa
     2521 gccactgcgc ccgcgtggtt cccgaaaaga agtcgcggat ctgggcggca ttgacgtcgc
     2581 tggaatcgtg acttgctccc acgtatgcta tcgccatccg gtcggaaagg tggtgttcct
     2641 cccccggcgg cacgagggac ctgcgagcga aggggggatt cgccgcatac cgccactccc
     2701 actggtgcac gccgccgaac gcggccgccg cctggtcctg catacctgtg ttgctgaaat
     2761 ggagtccgtc ctcaatgtgg tgcgccaggg ccgtcaattc ggcccgctgt tcatcagaca
     2821 gggtggcacc gcgttcgcca cgagcaaccg caatcgccgc tttgagagca cccagcaccg
     2881 ccaccatgag agtacctgaa cctccgaggc cggcgtaacg cggcgcctcg tgggcaagcg
     2941 ttaacgagac cccgtccacc gcgcagtggc tcactgcggc aaataccagc ccgaagcggg
     3001 aatccagagg cacctcatca gccgggaacg tttcgtaagt ctcgtcgttt ctcacaagaa
     3061 cccagcccgg tttgtacggt tcaaggcggg ctgatgtgcg catattcagg gcgatgttcg
     3121 cggtggaggg ccgaatgtgt tcatacggta gtgcgagcgc tttgagatcc cacgttccgc
     3181 tcgcgtcaac ccggcaggga gccgtggccg tgacggaaca gcgttcgagc gtagatgtca
     3241 gacacagtcg cgtttcgctt gtgacgttca caggaatcct gtcctttccc tgtcagaacg
     3301 tgatcaggtg ttttttctgt tcgatcacat cgccccggaa atcgagtgaa agaggcggca
     3361 atgatgtatc aactggcgag gcgaaactgc tgtccgggta gagctgtgcc ctgacgagat
     3421 tcagtcgctc caacacggcc tttgcttctt cgatcctgtt ggcctgccac agaaccacga
     3481 tctggttgac cagtgcgtcc tcattcatcg gcgcaacacg ttccgcgcgg gcgaaccagg
     3541 ccagcgatga gtccgtctgg tgcctgttcc agaacagaat ccccagccgc aggtaggcgt
     3601 ctacgcggtt gggatccacg agaacagcct tccggagaaa gcgctccgat ctctggaagt
     3661 tttcacgcat gtattcccag tagccagctt tgaagtaggc ttcgcccacc ttcatcattt
     3721 ccacccgcac ccgtgcggta tcgttctgtc gggtggcccg ttcggccaga agggtccgcg
     3781 cgtacgcaac cgcggcaacc ccgtcgctgt cgatcggcat gagggagagg agatgctcgg
     3841 cggttctggc acattcctcg agttggccga ggcggtaaca ttccagcgcc gccagcgagc
     3901 gaagtgcaag ctggtcatcg ggatgctggg ccagatgttc ccggtacaat cgcgcggctt
     3961 cgccccacct gccctgctgg acgtagacgg ccgcgaggtc tggcttcccc ctgcaccccg
     4021 ccactcccag ggatatcgcc agcaccagaa ccgtgatcag ccccctcatt gcacctccac
     4081 accgggcaat gtcttcaaaa gctcgcgcat tgctttcgca ttgatggccg cagaaccgcc
     4141 tttgcagttg ttgaagaaaa tgagaatttc gcgcacttgt ccggacagcc gtgctacctt
     4201 cccaacccat tccttgagtt cctcgtcgct gtaccggtag tcgtaacgat ctgccccgcc
     4261 accggcccac catgagtccg cgttgcgtcc gtgaaaacga atgtacgctg tcggcaccgt
     4321 gaacttggca accgggggca tcatttcccg caactgtggt tcgtccaccg acacaaaccc
     4381 aatggcgttt tcccgcagga atgcgcctac ctccggtacc agccagctcc ggtgacgaaa
     4441 ctcgacaaac cactggacat cgttcatgcg acccgtgact tcagccaaat actggaaatt
     4501 ccccggtgta ttcctgaaac tccacgggaa ctgcgccagc acaccccgca gaagccactt
     4561 ctcacgcagc ggttcaaggg cgctccggaa gggaccgctc gcgtcttcgt ccttgtattc
     4621 atgggtggtc agccggttgg ctttcacgac gattccatgg gtttccggca ttttctcagc
     4681 cagacgttgc atcgagtctg ctgatgggat gcggtagtac gaggaattca gttccagaac
     4741 ccggaaggac ttcacgtagt agtccaacca gcgcgacctt ggcaacccgg aaggatagaa
     4801 cactccctgc cagtcgtcga aatgaaaacc cgaggttccc acccagatac gtgtgggagt
     4861 ttcttcagag tgaacctttt ccacacctgt tccgctcatc ttgcctggca atttgcgaat
     4921 cgtttctcga caaaactgct caaggtgttt acagaaagat caaacccgca tatatttctt
     4981 ggcaacggag caggcaacgc gtgcttttgt ccgggacgtc acggcgtacc accgtcccgg
     5041 ttccctacgc ggttggcaga cgccccgctg ccctttcgcc caccatacaa acgtatgggg
     5101 cgtggtctgc ggggaaatgt ctcatttcca gaaagaggag aatcggaatg aaaacagctc
     5161 ttacccgtag tatcgttctt gtcgctgtga ccgcgaccac cctcgtgctg ggcggttgcg
     5221 gtttgagcag ggctgtcaag ggcggcgcga ttggcgcagc cacaggtggc gcgattggag
     5281 ctgccatcgg taaccgcatg ggcagcaccg ccaccggagc gatcgccggc gcggctattg
     5341 gaggaaccgc aggcgccgtg attggtcact acatggaccg acaagcggag gagatggcgg
     5401 caaatctgga tagcgcaagt gtcgagcgcg tcggagaagg gataaagctc acattcggtt
     5461 cgggtattct tttccgatcc ggttccgcgg atctgactcc ggaagcgagc gcgaatctcg
     5521 tgcaactcgc agacattctc aagaagtatc ctgacacaaa tctgattatc gaaggtcaca
     5581 cggattcgga tggctctgag tcgttcaatc agacactgtc cgagcggcgc gcgaacacgg
     5641 ttcgcactgc gctcgtcaac aatggcgtgg ctgcggagcg cttgaccgca gtggggtatg
     5701 gcgagagcca gccggtgatg gctaacgata cacctgccgg gaaagccgcg aaccggcgcg
     5761 ttgaaatcgc cgtttttgcg aatgacaaat tgaaacagga agctcaggcg agcgcagccc
     5821 agtaacctgt gcgattcgtg atctgacgcc ccggtgggga aaccctccgg ggcgtcgctt
     5881 ttctgagcag aaatgggcac gggtttccag atctgaagcg ttgccggagg catgtcggca
     5941 cagaagggat ccctctgcgc agtgtttttc gggggagatc gaaatccgcg catcgcaaga
     6001 aggacgttgc cgtgaaaaac tcgctaccaa agggcaccac gctgatcgcg tgtatcggca
     6061 tcgcgttagt aacgggttgc gccaacctga cctggtgcgg tctgaagacg gccgagcgca
     6121 tagaaccggc ggtagaacgt gtcttgcctc ctgctccgat tgtggcacca gttccgacag
     6181 cgccggcgga accggttgtg gtaccggcac ctgccaccgt tgttgaagcg cctcccgcac
     6241 cgccaccccc gccacctccc ccaccggtcg tatcggtacc accggtcgta ccggcagcgc
     6301 cgccacctac ggaggtggag gtgtacatgg agcggcaggc ggacgaaata accaccgacc
     6361 tgcggagcgt cgccacggtt cagcgcaccg gagaacagat caaaatcaca tttggctcgg
     6421 gagtcctttt cggacccggg tcggccgcgc tgacggccca agcgcaggag agtatcgcca
     6481 aactggccgc gatcatcaaa aagtaccccg acacgaatct tcgggtcgaa ggtcacacgg
     6541 actccgatgg tgccgaggcc tccaatcagc ggctgtcgga acgacgagca aatgccgttg
     6601 cggaagctct gcggagcaat ggcatcccgg ctgccagggt tacggcgata gggtacggtg
     6661 aaacgcggcc tgtcggatcc aacgacactc cggagggcaa ggcggctaat cgacgagttg
     6721 agatcaccgt caccggcggc gcaatactga gagagggatc atcgagataa cgctccgatc
     6781 ggattcatct gacgcctcga cgatgtccct cgtcggggcg ttctttgtgt ggccattgac
     6841 gcaatgagtt tgctacagcc tctttcgctg tctatatttc cctcgcgatg cgagactttt
     6901 gttttgcttt tgcagttgta cccagaagct tacgcgatct gaatctttct gagacgtttc
     6961 cggtcggtgc gtgtcgccga cgtttccagc aacggattcc cgcgggctga tcaaatcgcc
     7021 gttagcgggg atctcactcg ccgaaaggag aacctgtatg agaaacgcat ctgcacgtgt
     7081 cggatgggtt ttcgcggctg ctctgagcgt gattgcagcc ggatgctgcg ggttgaacac
     7141 cgcccccgga accgcacgac ctgcgtcaga cgcaaccgcg gtgaacgctt acatggatca
     7201 gcaggtcggc gagatgcggg gggaccttga gaacgcccag gttcagcgcg aagggcagag
     7261 aatcaaggtt acctttgggt ccggtgtgct gtttggaaca ggatccgccg agcttactcc
     7321 cgccgcgagg gagaacattt cgcgcctggc aggaattttg aagaggtacc ccaatacgaa
     7381 cgtgagcgtt gaaggtcaca cggactcgga tggcgctgag gacttcaacc aggcgctttc
     7441 ggagcggcgc gcgaatgcgg tggcgcaggc gctgcgtgac gaaggtgtgg ccgggtcgcg
     7501 cattcggacg gttggattcg gggaaactca accgatcgcc acgaacgaca cgcccgaggg
     7561 gaaagccgcc aaccgccggg ttgagatcat cgtgatggca aacgaaagtt tcgggggacg
     7621 ctaagcgttc tgaaccacgt gcctaacgcg aagtgcgccc cgacggacga atgctgtcgg
     7681 ggcgcgttgc ttttttcatt gacagatgat ctacagaata gttgccccgg aagcgggaga
     7741 aattcttgtc agccccaaaa cggggtcggt gtcactcgcg tacaacggca atcccataga
     7801 ctatttcgat cgtgatggac gtctgctcgg aagccaggtc ggagaccggc actaccgccg
     7861 gggtttggac aacaggcttc ttgagaaata ccgaccaccg gaagaagggg aacacgtcag
     7921 gaatgaggtt ccggaacctg cgcgccaacg attgatcacg gcagcgtacg agcgggcggg
     7981 aatgcttgcg gacgcagtga gccgaagctc catcgaatgc agaccgacac acccgagtga
     8041 tatggcgctg ccgctttcgg ctcgagaggc tctcgcaggt tttctgaacc ccatcctggc
     8101 gcagggtttc gaacgactgt gtgtcgaggc aatggagttc caacgtatgt acgctcctgt
     8161 cagcattctg ccccccgatc agtatttgag cgtggttgcg cagttgacca ccggttgctc
     8221 ctacaaccgg tgcacattct gcaacttcta caaatcacgg gggttcaccg tcaaatcagc
     8281 ggatgagttt cgccgccatc tgagtgaagt tgtctcgttt ccggccacct tcgcccgtct
     8341 ccggaaatcg gtgttcctcg cggacgggaa cgcgctcagc ctgccgcaca cgaagctgct
     8401 ggaaatgttt ggcgtgctgc acgagttctt ttcgttcgtt ccttcgcagg tgtgtgagat
     8461 acgcgagcgg tatgcgtaca ggaagagcca tcctcactca tatgaaggag tcttcagttt
     8521 cgtggacggt cgcgccgcga tggaaaaaac cgtggatgac ttccgcgagc tgaaggaaca
     8581 gcatctccgc agggtttacg tcgggctgga aagcggggac gacgctctgc tgacgttcgt
     8641 tcgcaaaccg gcaacgcgct cgaaaatcat agacgcggtc cgccgcatga aaacagccgg
     8701 attgaacgtg ggagtaatt
//
LOCUS       DAOSOO010000137         8700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8067_length_8700_cov_5.662117, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000137 DAOSOO010000000
VERSION     DAOSOO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_8700_cov_5.662117"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLT86_09945"
     CDS             <1..919
                     /locus_tag="PLT86_09945"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOT36973.1"
                     /translation="VFTHGLAHSEEAPQVETHSSAREGAPALPLPAAREGNFSLDDLP
                     PYRHQGISRGRLAIALAGVGVYDVAFYETLKKPWWSGTKSDMHVINDWWNGYAMEVDK
                     AAHAFAGQSMARLAAESYEWTGMTRTQALFWGGVTSLATLSQVEVLDGYTAKFGFSTA
                     DYAANIAGAFFPLAQEMWHPLKTVTFKISYHTRRFEEHGNNGNILEDYDRQTYWLVLN
                     LKETLPRTVSSVVPAWLGIAAGYGVENAFASTGRMREYYLALDIDPTRANVGPGWLSR
                     ALAPLRYVHLPSPAVRFREDGTRFFALYF"
     gene            1096..2244
                     /locus_tag="PLT86_09950"
     CDS             1096..2244
                     /locus_tag="PLT86_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT36974.1"
                     /translation="MALVDLRGQKKRRTPEEMIAHLNSFSLDLAFSAGVWFFAPGGGR
                     FHDRYTPNLTIKERLEVAAELSRYGLKAVEAHYPNEVNEDNVHLYKDLEKNAGVRLAT
                     VIPGLFFDSEFEWGSLSSPIPSVRKKAIDRTIAALKLNKELNTDFAIVWPGIDGYENP
                     FGMDMVAARDRFAEGLAEAMDAVPGIRVAEEPKPYEPRGHILYGTTAEGLLLCAKVES
                     LLKAPENVKLLAEGHAMMCMNPEVGHMLMGFEDAPYSFSLIMEYGRLAHTHWNSQPLG
                     NYDQDLNVGVLSPESTEASLYVLKMYGYKGYFGPDINPERMPVQQALKNCFDALRAAN
                     ERINNLDHEKIIMATSHPEKNRGWLEAYLIRERAPKSISLPPLPPFEK"
     gene            2305..3510
                     /locus_tag="PLT86_09955"
     CDS             2305..3510
                     /locus_tag="PLT86_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36975.1"
                     /translation="MPSLRIRGLVRAVRYVETRMRGRLTLNDADRLAHYVRSVVAEVR
                     AICQNYGTRPEALPRPSREAFAFLNDLDTAHLDRVKPRPVAVSTPVRISSVVRLTGAV
                     LTDLGSGTEDESSECFVERCHARVLAAAGEIEALCRARGVDPREMAVRSRRAYGLLRY
                     LSNVQTTRAYVRTAANLKRICGELRRREGADSSAPVVTLDDHSHIYRCGRGKDAPLVL
                     HLSPAFIGAPEEVLESVARLAFGIGAASDKKAVHTFIHGPGALAIIQEIAELTSGPSE
                     TRGAVYDLREVCERVRNRYFDDPPDPPSSLAWTDSLTYRMFGLYSSVRDRITISRSLD
                     SPKVPGYVIDYVMFHELLHKKHGIGWASKQRTMHTSRFRRDEHAFPRFKAAQEFLNRL
                     SSQMRRRLT"
     gene            3661..5013
                     /locus_tag="PLT86_09960"
     CDS             3661..5013
                     /locus_tag="PLT86_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT36976.1"
                     /translation="MRKVIVAWCVGVVFFDAVAGAPAVGMFRSLGASPLWIGLLAAIP
                     PIATLAQPVASYIVARTGSRKRLFLRSAYPGRVIWLPIVLCGYFLPRGDLSIAFLFVL
                     VFFSKLSDSISGPAWMAWISDLVPENERGRFWGRRQMWASLCSVTGSLCLGYYLGTQP
                     PYPKFIVFFGIVAFLGWLDVFIHRAVPGVRLDVPSKRPDFRELVTVPLRERSYRSLVL
                     FYGLFSFSVAVGGALFPLLLIEEFGFSYFKISFFFGIQAGFMIASSHLWGRFMDNVRE
                     GPRVVFLMCSVIIALLAPPWTMIPVGQNLLAGLNFALGGIGWGGYQIAFTGLVIAMSP
                     RESRAMYFALAAVAAGVGNAVGAVCAGLIAEQLAGFSFFWGPFKLTQYRTLYLISGIS
                     RMSCLLLLPLIRQPESAPVGTYFRRAMSLNPMEPGTYAYLWKKFGKPNGNEQRGETEN
                     "
     gene            5190..5744
                     /locus_tag="PLT86_09965"
     CDS             5190..5744
                     /locus_tag="PLT86_09965"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOT36977.1"
                     /translation="MELKHPDLVAEALRLGATKAAMLPLEGLRFYPELRTLCERNACG
                     SYGKSWGCPPHVGEIEELAARVRSFAHGLLFQCITEIEDSYDIEGMGRGAAHHKRVVR
                     ELAEIFRTRYGAPDVLVLGTGPCDFCEECTISTGQPCRYPDRLISSAEAYGMNVKELV
                     ERCGFSYINGKNTVSYVGVILFNG"
     gene            complement(5712..6686)
                     /locus_tag="PLT86_09970"
     CDS             complement(5712..6686)
                     /locus_tag="PLT86_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT36978.1"
                     /translation="MELERSFSTHEVAELFGINVSTVKRWADSGDLPCMRTNGGHRRF
                     RLRDVVEFARSHELEPATLRPIVEGLGDEAAGVGAEAPILNGNWVALREIVQRYALQG
                     DGTRLEELFGAVLVSGWHPAELCDHVIAPTMEYIGKQWAQNRLTVADEHLATHAITTA
                     LGRLPHMQEKSGRSPHRALCGTFAPDLHELACSCVALVLRFEGWDVTVLGAATPSDSF
                     VNCVERLRPDLVCVSATVIYDPVAFRTDCERLSLASLAVGNKYVIGGTALAEANLVCP
                     HGVARILKDMRGLVEYVRSEFPRNAARKTDGEEGDQSGSAVEKNDSDV"
     gene            6955..7596
                     /locus_tag="PLT86_09975"
     CDS             6955..7596
                     /locus_tag="PLT86_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT36979.1"
                     /translation="MQTSDRETRRREFEEIALPLQASLSRMALAMTRNPVEAADLVQE
                     TFYKAWRFFPAFRQGSDCKAWLTAILRNAYINRYRRIQRRPPQVEFDEWQEMDPSFRD
                     DISSFPAPDDDVALGDALSDEVLDALDQLPRHFRDVVVLSDVEGLSYQEVADFVGCPI
                     GTVRSRLFRARAMLRGLLNDYAAENGVGKSSDTVEDLIAELDEASMVEATVAE"
     gene            complement(7710..>8700)
                     /locus_tag="PLT86_09980"
     CDS             complement(7710..>8700)
                     /locus_tag="PLT86_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT36980.1"
                     /translation="ALKIGIVGMGGIGNTHARVYMDDPLAEIVAVCDIIKERADKAAK
                     DYNARAFYSVKEMLDSGIKLDACSMCTAGKENGGDHHVPTMELLGAGIPVLGEKPISN
                     EIEKARMQVALAKEKNIPYAINLNHRFTPAAVKAKEWITSGRLGQLHIMNMTMWINNP
                     NESAEWFHLRALHPHSFDVMRYFCGPVKSVQAFMMKGKGRTIWSNTQVNLLFENNVVG
                     HLTGSYDAGGSYGLERAEVVGSEARFVLEDACEILTFYPRRSIEKEVYYHVGGMQSFG
                     NTFQSRIHRWLEQLTAGVKPEDVDGSGADGLAVQEIIEAAIKSWQTHSVVDLT"
BASE COUNT         1720 a         2526 c         2551 g         1903 t
ORIGIN      
        1 cgttttcacc catggactgg cgcattcaga ggaggcgccc caggtggaga cgcactcatc
       61 tgcgcgcgaa ggggctcctg ctctgccttt gcccgccgca cgggaaggga acttctcgct
      121 ggacgacctc cctccatatc gccatcaggg tatttcacgc gggcggcttg ccattgctct
      181 cgcaggagtg ggtgtgtacg acgttgcgtt ttacgaaacg ttgaaaaagc cctggtggag
      241 cggaacgaaa tccgacatgc acgtcatcaa tgactggtgg aacgggtacg cgatggaggt
      301 ggacaaagct gcgcacgcct ttgcggggca atccatggcg cggctggccg cggaatcgta
      361 cgagtggacc ggaatgacgc gcacgcaggc tctgttctgg ggaggcgtaa cgagccttgc
      421 aacgttgagt caggtcgagg ttctggacgg ttacaccgcg aagttcggtt tcagcaccgc
      481 ggactatgcg gccaatatcg cgggcgcgtt tttcccgctc gctcaggaaa tgtggcaccc
      541 tctgaagacg gtaacgttca aaatcagcta ccatacacga cggtttgaag agcatggaaa
      601 caacgggaat atcctcgaag actatgaccg ccagacctac tggctggttc tcaacctcaa
      661 ggaaacactg ccacggacgg taagcagtgt tgtgcctgcg tggttgggaa tagcggcagg
      721 gtacggcgtt gaaaacgcat tcgcctccac agggcgtatg cgtgagtatt accttgcctt
      781 ggatattgat ccgacgcgtg caaacgtcgg ccccgggtgg ctttcccgcg cgctggcgcc
      841 tttgcgttac gttcacctgc cctcgcccgc cgtccgattc agggaggatg gcacccgctt
      901 ttttgccctc tacttctagc gaacgtgcgc caagcctggg gcgcataccc tccagcggat
      961 ccgtgtgtct ggttgtgcct tgcatgtata tttgactctg ctgggttgaa cggacggaag
     1021 gatttcggca accgactctg aaaggcacgc gtaggtacct gaacagagcc gatcctgcaa
     1081 agaaggaggg ttcctatggc actcgtagac ctgcgaggac aaaagaaacg tcgtaccccg
     1141 gaagagatga tcgcgcatct caacagtttc tcgctcgacc ttgcgttttc cgcgggcgta
     1201 tggtttttcg cgccgggagg tgggcggttc cacgaccgct acacgccgaa tttgacgatc
     1261 aaggaacgcc tggaggtcgc ggcggagctc tcccgttacg gactgaaagc agtcgaagcg
     1321 cactatccga atgaagtgaa tgaagataac gttcatcttt acaaagacct tgagaagaat
     1381 gcaggcgttc gtctggcaac ggtaataccg gggttgtttt tcgatagcga attcgaatgg
     1441 ggatcgcttt cctctccaat cccctccgtt cggaaaaagg ctatcgaccg cacgattgca
     1501 gcgctcaaac tgaacaagga actgaacacg gatttcgcaa tcgtgtggcc gggtatagac
     1561 ggatatgaga atccgtttgg catggatatg gttgccgccc gggatcggtt tgccgaggga
     1621 cttgccgagg cgatggacgc agtgccggga atcagagtgg ccgaagaacc gaaaccctac
     1681 gaaccgcgag gacacattct ttacggaacg actgctgaag ggctgcttct gtgcgcgaaa
     1741 gtcgagtcgc ttttgaaggc tccggaaaac gtaaagctcc tggccgaggg gcacgcgatg
     1801 atgtgcatga accccgaagt cggtcacatg ctgatgggct ttgaggacgc accctacagc
     1861 ttcagcctga tcatggaata cggcagactg gcacatacgc actggaacag ccagccgctg
     1921 ggcaattacg accaggattt gaacgtaggc gtgctttctc cggaatcgac agaggcgtcg
     1981 ctctacgttc tgaagatgta cggttacaag gggtatttcg ggccggatat caacccggaa
     2041 cgcatgccgg tgcagcaagc gctgaagaac tgttttgatg cgctcagagc ggcaaatgag
     2101 cggatcaaca atctggacca cgagaagatt atcatggcga cgtcacaccc cgagaagaac
     2161 cgcggttggc tcgaagcgta tctcatccgt gagcgcgcgc cgaagagcat atcgctgccg
     2221 ccgctcccac cgttcgaaaa atgacctcgt gattggtgcc ggtcggggcg tgaaaccccg
     2281 gccggtgcct ctcggcgccc atccatgcct tcgttgcgaa tccgtggcct tgtccgagcc
     2341 gtgcgctatg tcgaaacacg tatgcgcggc aggcttactc tgaacgacgc cgacaggctg
     2401 gcgcactatg ttcggtcggt agttgcggag gtacgggcaa tatgccagaa ctacggtacc
     2461 aggccggaag cgctgccacg gccttcgagg gaagccttcg catttttgaa cgacctcgat
     2521 acggcgcacc tcgaccgcgt gaaaccccgc cccgtggccg tcagtacacc tgtccggatt
     2581 tcctcagtag ttcgcctgac aggggcagtg ctgaccgatc tgggctccgg cacagaagac
     2641 gaatccagcg agtgttttgt ggaacgctgt cacgcccgcg ttctggctgc cgcaggggaa
     2701 atcgaggcct tgtgccgggc gcgaggagtt gatccccgtg aaatggccgt gcggtcccgc
     2761 cgagcgtacg gtcttctccg ctacctgtcg aacgtccaga cgacacgcgc atacgtgagg
     2821 actgcggcga atctgaagcg catctgcggc gagctaagac gacgtgaagg agctgattcc
     2881 tccgcaccgg tggtgaccct ggatgaccac agccacattt accggtgcgg gcgagggaag
     2941 gatgcgcctc tggtgctgca tctcagcccc gcgttcatcg gcgcgcccga ggaggtcctg
     3001 gagtctgtgg cgcgcctggc gttcggaatc ggagccgctt cggacaagaa ggcggtacat
     3061 acattcatcc acggccccgg ggcgctggca atcattcagg aaattgccga gctcacgtcc
     3121 gggccgtccg aaacacgtgg agcggtgtac gaccttcgag aagtgtgcga acgtgtgcgc
     3181 aaccgatact tcgatgatcc gcctgatccg ccttcttcgc tggcgtggac ggactccctg
     3241 acgtacagaa tgttcgggct ctacagctcc gttcgcgata gaattacgat cagccggtct
     3301 ctggacagtc cgaaggtgcc tgggtatgtg atagattacg tgatgttcca cgagcttctt
     3361 cataagaagc atggcatcgg ctgggcatcg aaacaacgca cgatgcacac cagtcgcttc
     3421 cgacgggacg agcacgcatt tccccgtttc aaagccgcgc aggagtttct caacagactt
     3481 tcctctcaga tgcgccgccg gcttacctga catgatttgc cccgtcctca ccgggccgtt
     3541 gtcccgacaa gatcacactc tggctgggta actgcagtgt tgacgtcgcc gcgtgaatgg
     3601 tttcgctatg cacccaccct gccgggcgag caactgtcaa tcgacgaacg gcaccgcggc
     3661 atgcgcaagg ttatcgtggc gtggtgcgtc ggggttgtgt tcttcgatgc cgttgccggt
     3721 gcgcccgcgg tgggaatgtt ccgttctctg ggagcctctc cgctgtggat aggcttgctg
     3781 gcggcgattc ctcccatcgc gactctcgcg caacctgtcg cgtcgtacat cgtcgcgcgg
     3841 acgggttccc ggaaacggct gttcctgcga agtgcgtatc ccggacgggt catctggctg
     3901 cccatcgtcc tgtgcgggta tttcctgccc cgtggggatc tctcgatcgc gtttcttttc
     3961 gttcttgtgt ttttcagcaa gttgagcgac tcaatatccg ggccggcatg gatggcatgg
     4021 atctccgatc tggtgccgga gaatgaacgg ggacgcttct ggggcaggcg ccagatgtgg
     4081 gcaagcctct gcagcgtaac cggttcgcta tgcctcggat attacctcgg aacgcagcct
     4141 ccttacccga aattcattgt gtttttcggc attgtcgcgt tcctgggctg gttggacgtg
     4201 tttatccatc gcgcggtgcc aggcgtgcga ctggacgtgc cttcgaaacg gcctgatttc
     4261 cgtgaactgg tgacggtgcc gctgagggag cgctcgtacc gttcgctggt gcttttctac
     4321 ggcctcttct ccttctcggt agccgtcggc ggtgcgctgt tcccccttct tttgatcgag
     4381 gagttcgggt tctcgtattt caagatttcc ttctttttcg ggattcaggc cggattcatg
     4441 atcgccagtt cccatctctg gggccgcttc atggacaacg tgcgggaagg gcctcgcgtg
     4501 gtctttctga tgtgttcggt aatcatcgct ctgctggcgc cgccgtggac gatgatcccg
     4561 gtcggtcaga accttctcgc gggactcaac ttcgcccttg gcgggatcgg ctggggaggg
     4621 taccagatcg cgttcaccgg gcttgtaatt gcgatgtctc cccgcgagtc gcgggcaatg
     4681 tacttcgcgc ttgcggccgt cgccgctggc gtcggcaatg ctgtgggcgc cgtatgtgcg
     4741 gggttgatcg ccgaacaact cgcagggttc tcctttttct ggggcccctt caaactgact
     4801 cagtatcgaa ccttgtatct gatttccggt atctcgcgca tgtcctgcct gctcctgctg
     4861 cctctcatcc gccaaccgga gtcggcgcct gtcgggacat acttccgacg ggcaatgagt
     4921 ctcaatccaa tggaaccggg aacgtacgcc tatctctgga agaaattcgg aaaaccgaac
     4981 ggcaacgagc agcgtggaga gaccgaaaac tgacgtccca catgcccgga acggagctct
     5041 tttttgattt tcgcgttcgg caggggctgc cgtccggggt tttgcctgcc ctgcttcgga
     5101 ccgccgttct ctggaacaaa ttggttgcgg tacactgtta ttgctgaacg agattcgcac
     5161 cagacctgca tcgcccggag gtgattctga tggaactgaa gcatcctgac cttgtcgccg
     5221 aagcccttcg cctcggcgcc acaaaggccg ccatgttgcc tctggaaggg ttgcggttct
     5281 acccggaact gcgcaccctc tgcgaacgga atgcctgcgg gtcgtacggc aagagctggg
     5341 gctgccctcc gcacgtcgga gaaatagagg aacttgccgc gcgggtgcgg tcgttcgccc
     5401 acggactgct cttccagtgc atcaccgaga tagaagactc gtacgacatt gagggaatgg
     5461 gacgcggcgc tgcacaccac aagcgcgtcg tgcgcgaact ggctgagatt ttccgcaccc
     5521 ggtacggggc gccagacgtc ctcgttctcg gcacggggcc ctgtgatttc tgtgaggagt
     5581 gcacgatctc cacaggccaa ccgtgccgat atccggatcg gttgatttcc tccgcggaag
     5641 cgtacggaat gaatgtgaaa gaactcgtag agagatgcgg tttctcgtac atcaacggga
     5701 agaacaccgt ttcatacgtc ggagtcattc ttttcaacgg ctgagcccga ttgatcgcct
     5761 tcttctccgt cagtcttccg tgcggcgttt cgcggaaact cggaccggac atattccacc
     5821 agcccgcgca tgtccttcag aatccttgca actccatgag ggcacacaag gttcgcttcc
     5881 gcaagcgcgg ttccgcctat gacgtacttg ttcccgactg ccagggaggc gagggataac
     5941 cgctcgcagt ccgtgcgaaa ggcaacaggg tcatagatga ctgtcgctga tacgcagacg
     6001 agatcggggc gcaaccgttc aacgcagttc acaaacgaat ctgagggtgt agccgccccg
     6061 agtaccgtca cgtcccaccc ttcgaaccga agaaccaacg caacgcacga gcacgccagt
     6121 tcgtgaaggt ccggggcgaa ggtgccgcac aacgctcggt gtggggatct gccagacttc
     6181 tcctgcatat gcggcagtct tcccagtgcc gtggtgatag cgtgagtggc gaggtgctca
     6241 tcggcaaccg tgaggcgatt ctgtgcccat tgtttgccga tatattccat ggtcggagca
     6301 atgacatgat cacagagttc cgcgggatgc caaccgctta ccagaaccgc cccgaagagc
     6361 tcctcaagtc tcgtgccatc gccctgtaac gcatagcgct ggacaatctc cctgagcgca
     6421 acccagtttc cgttgagaat cggagcctcg gcgcccacgc cggccgcttc atcgccgagc
     6481 ccctccacta tcgggcgcag tgttgcaggc tcaagttcgt gggagcgggc gaactccacc
     6541 acatcgcgca accggaagcg ccggtgcccg ccgttggttc tcatacacgg cagatcaccg
     6601 ctgtcggccc accttttcac cgtcgacacg ttgatgccga acagttccgc tacctcgtga
     6661 gtggaaaagc ttcgttcgag ttccacggac cgcctccatg agcgttttga gcgtttttag
     6721 acgaggtaac accctgcgaa tctctgcgga ttcgccgccg ttttcagggc cttttccgcc
     6781 gtatgcgggc ggtgtggtac gccataagta tatacttgtg cgtcaattat aattttaaca
     6841 acaaaaatct taaaaaagta gttgacacgg gtgtgcagac actgtatact ttaatcgtgc
     6901 aaaccgttca acacctacgg gcaacggagc atctgccagg aagcgaggaa cacaatgcaa
     6961 acttccgacc gagaaaccag gcgccgtgaa tttgaagaga tcgccttgcc tctccaggct
     7021 tccctttccc gcatggccct tgccatgacg cgaaatccgg ttgaggcagc ggatctcgta
     7081 caagaaacgt tttacaaagc ctggcggttc tttcccgcgt ttcggcaggg ctctgattgc
     7141 aaagcgtggc tgacggcaat actccgaaac gcgtacataa acaggtatcg aaggatacag
     7201 agacgcccgc cacaggtgga gtttgacgag tggcaggaga tggacccgag cttccgggat
     7261 gacatttctt ccttcccggc tcctgacgac gatgtggcgc tgggtgatgc gctcagtgac
     7321 gaagttcttg atgcgctcga ccaacttcca cggcacttcc gcgacgttgt cgtgctgtcc
     7381 gatgttgagg ggttatccta tcaggaagta gctgatttcg tcggatgccc tatcggcacg
     7441 gtacgatccc ggctgttccg cgcccgggcg atgttgcgcg gactcctgaa cgattacgct
     7501 gcggaaaacg gggtgggaaa gtcctccgat acggttgaag acctcatcgc cgaacttgat
     7561 gaggcatcaa tggttgaggc cactgtcgcg gaataagcac caaaacgttc gcaaacggaa
     7621 agcggggagc agcttcgacg ctgctccccg cgtgtttttt ctcaacggcg tggcgcacat
     7681 tcgtgcacca aaacacctca tcattcctgt caggtcagat caaccaccga atgagtctgc
     7741 caggatttta tagcggcttc gatgatctcc tgcaccgcca gcccgtcggc gcctgaacca
     7801 tcgacgtctt ccggcttcac gccggccgtc aactgctcca gccagcggtg gatgcgcgac
     7861 tggaaggtat tcccgaagct ctgcattccg ccaacgtggt aatacacttc cttctctatt
     7921 gaacgccgcg gatagaaggt aaggatttcg cacgcatcct cgaggacaaa ccgcgcctcc
     7981 gatcctacca cttccgcgcg ttcaaggccg tagcttcccc ctgcgtcgta gctacccgtc
     8041 agatggccca caacgttgtt ctcaaacagc agattgacct gggtgttcga ccagatggtg
     8101 cggcccttgc ccttcatcat aaaagcctgc acgctcttca cgggcccgca gaaatagcgc
     8161 atcacgtcaa acgaatgggg atgcaacgcg cgcagatgga accattccgc gctctcgttc
     8221 gggttgttga tccacatggt catgttcatg atgtgcaact gcccgaggcg gccagaggtt
     8281 atccactcct tggccttgac ggcggcggga gtgaatcgat ggttcagatt gatggcgtag
     8341 gggatattct tctctttggc cagcgcaacc tgcattcgtg ccttctctat ctcgttcgaa
     8401 atcggctttt cgccgaggac cggaatgcct gccccgagca actccattgt aggcacatga
     8461 tggtcgcccc cgttttcttt gcctgccgtg cacatactgc aggcatcgag cttgatcccg
     8521 ctgtcgagca tttctttgac gctgtaaaac gcacgggcgt tgtaatcctt cgcggctttg
     8581 tccgcgcgct ctttgatgat gtcgcacacg gcaacgattt ccgccagcgg gtcatccatg
     8641 tagacgcgcg cgtgggtgtt gccgataccg cccatgccga cgataccgat tttgagtgcc
//
LOCUS       DAOSOO010000138         8567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8199_length_8567_cov_6.040061, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000138 DAOSOO010000000
VERSION     DAOSOO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_8567_cov_6.040061"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1059)
                     /locus_tag="PLT86_09985"
     CDS             complement(43..1059)
                     /locus_tag="PLT86_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOT36981.1"
                     /translation="MSAALPITEFMLKNYRNFNARATRDAFIAYWRHYERGGRMFWAV
                     AGAMSSAQLGITLAPAIRAGLIHGMSVTGANLEESLFRLVAHEHYRDFPDYRYFRKED
                     DTRILNDRMRRVTDTSIPEDEAFRAVEKIIVPMWTEATRKGARRLWHEYFYDLVRAVG
                     PELSSGPREECWLLAAAEAGLPVVVPGYEDSTFGNIFASYVKTGECSANIVKSGIEYM
                     AWFYDEYRKLSEGAGVGFFQIGGGIAGDFPICVVPSIKYDLQQPVKPWAYFCQISDST
                     TSYGSYSGATPNEKITWDKLTEDTPMFVIESDATIVAPLMLRALLECVAHPAEANAMI
                     AAAA"
     assembly_gap    1348..1447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1651..>1867)
                     /locus_tag="PLT86_09990"
     CDS             complement(1651..>1867)
                     /locus_tag="PLT86_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36982.1"
                     /translation="EEGGLRAGNRMDRSPFFCSLTPAPAAREPVEVPGAQQSSQAGRR
                     DAWSVISIHRTGAERRFAPVPRRFATR"
     assembly_gap    1868..1967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2199..7658)
                     /locus_tag="PLT86_09995"
     CDS             complement(2199..7658)
                     /locus_tag="PLT86_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36983.1"
                     /translation="MKKLLTILGAVVMFATGEAWGANHTFALAGSPAAGELPANTSEI
                     FVFRGSVKTANNTAITVNSIRFSAGGTAVVPTEVDQVKLYEDTNANGAFDVGVDVPLG
                     TAQTFVSGNNTVNFSGLNWAIAKKTTKYFLVLVRTTSNVTVGHTFTLGIAAATAIDIT
                     AGQTVVGTFPLTSSAFTVWGLGASLGATTPAGDSMATDRVYLKSVFQFSLTATGETVN
                     VNSITITSAGSVTSSDVNQVSLYGDANANGIYDAGETLLGSTQSFAGTMTFTGSPLVA
                     VTTATPVNIVAVARATGNATAGRTLSLSLAANGDVSGTGATTSLSRPSQGTATSNTMT
                     FSSGDGVLTVSAGTSPPSAGVMAKSTSDIGVFHFKLSAAVEQINITSVKLTKGGTASV
                     PTELDLVTLYKDKNNNGLLDVGTDSLIGQQELGATNTFSGVTETRVPAGGSIQFIAVV
                     RTTANGVAGNTVTLSIAAGDVVGTGSTSGHTINSLGTAVSNAKTFSKLGVEIGANTPP
                     SGLLAKNKTYSLLQAKLTAGAENINVSGVTVTVSGTATLPGQIASVSLYIDANNSGTF
                     DTGETQLGVTTTAASTVTFSGTNFPLLVTAGGFVNVVATVKTANTALPAGVTCRLSIA
                     ADGDITGTGVTTGQSQSSVGTATGEQFHVANPNGVLGVEIGDTPPTGGAIQINTNDIE
                     IFQFAFAGPGDAAASVSSITLTAGGTVVPSDEIAEVRLYQDLNQDGTLDANDGVALGT
                     QSFAPSVTTVTFSSLTLSVPKNETVVMLAVVRTGAGGVSGHTFTLGLNATTDITATGG
                     TVTFTPPLTSNTFTFPGYLTTALGANTAAADTMHTFNGAHLKSVFQFAVTAVGQDMNL
                     TSVKLTAGGTAALPTEIAQISLYSDANNNGQYDVGETQRGTTQTAAGTVTFTISGLTI
                     TAGATERFVAVAHIPSDISAAANGKTFNLSIAAASDVVGANASGYYRASEGTATSNTM
                     TFYDGTKGVLNVSNGTSNPGAGRLSNSATFEVFHLKLSPLGEAFDLSKSDLSYNKVAI
                     KLGGTATFGSGQDIESMSLFDSDGSTPLGSAQTSATNGIVTFTWTSPAIQIASGGSRD
                     LIVKVTTGTGAQGRTLTLSIPDTGYVVGRGVTSSKWIGSAGTASSATWSFSDLTVALG
                     PYTPAAAVLNNSATKVSVLQVKLGAVAENVNIDGATNYLKVTLSGLAGTTGFGTQITG
                     VSLWEDANNDGVADAQQLGATQTSATDNVVTFSWTTPTTQITSGGFKYVVVAVNTGTG
                     VAGNTLGLTVEAGQVQGTGANTGRTLYSTGSAVSNVFNFANPNDVVGVSRGNAPVSAG
                     QITINQDNIPMIQLRLAGPGNKAATVTGITFTNTGTGWAGYETAQVRLYYDNNTDGQL
                     DVNDNLIDTRTFTNNTVAFTGLNIPLAQNGSRTVLAVLKTNSNGISGRTLNLQLTTNA
                     AVTAISSGSVSTALSETPLTSASKTFATGQVTVTKAAELAPSGVNPGDRNVALMRLNF
                     TTSGPAVKISSIKVDNTYGVGTYDAADIDSVEIYRDGGDLSFSATGDLRVAAALVEGL
                     SGGSGTAAFTAGIGDTLRASAVGVFYVVYDVATTAVSTRQFGAKLANNSYVTMGLGTV
                     ASANFPIELGYERSLPVEFASFTAEAVGQVVKLAWATESETDNLYWIVERREVSDPAS
                     TFVQIHRLDGMGSTPYRTDYEFVDRSVVPGMRYAYRLTDVSRYGVRKTHDPVEVTAGA
                     ALGLALGNAFPNPANPATVWAYSLPDEQMVRLAVYNTLGQQVRVLVNGRVGAGTHQAR
                     WDITDDTGRAMASGVYFAVMETPTSRLVSRVTVVR"
BASE COUNT         1469 a         2815 c         2432 g         1651 t
ORIGIN      
        1 caccgtcacc gtcgttctgg atgagtaaag ggcggttccg cgttacgcgg ccgcggcaat
       61 catcgcattc gcctccgcag ggtgcgccac gcattccagg agcgcccgca gcatcaacgg
      121 ggcgacgatg gtggcatccg actcgatcac gaacatcggt gtgtcctcgg tcagcttatc
      181 ccacgtgatc ttctcgttcg gcgtcgcgcc cgaatacgac ccgtacgacg tcgtcgaatc
      241 ggatatctgg caaaaatacg cccacggctt caccggctgc tgcaggtcgt atttgatgga
      301 cggcaccacg cagatgggga aatcccccgc gatgccgccg ccgatctgga agaacccgac
      361 ccccgccccc tccgagagct tccggtactc gtcgtagaac cacgccatat actcgatgcc
      421 cgatttcacg atgttcgcgc tgcactcgcc ggttttcaca tacgacgcga agatgttccc
      481 gaacgtcgaa tcctcgtacc ccggcaccac caccggcaac cccgcttccg ccgccgccag
      541 cagccagcac tcctcccgcg gcccggagga aagctccggc cccaccgccc gcacgagatc
      601 gtagaaatac tcgtgccaga gccgccgcgc gcccttgcgc gtcgcctccg tccacatagg
      661 cacgatgatc ttctccaccg cgcggaatgc ctcgtcctcc gggatgctgg tgtccgtcac
      721 gcggcgcatg cggtcgttca ggatgcgcgt gtcgtcctcc ttgcggaaat agcggtagtc
      781 cgggaaatcc cggtagtgct cgtgcgccac cagccggaag agcgattcct cgaggttcgc
      841 gccggtcacc gacatcccgt ggatcagccc cgcccggatc gcaggcgcca gcgtgatccc
      901 gagttgcgcg gaggacattg ccccggccac tgcccagaac atcctccccc cccgctcata
      961 atgccgccag tacgcgatga atgcgtcccg cgtggcccgc gcgttgaaat tccggtagtt
     1021 cttcagcatg aattcggtaa tcggcaacgc agcgctcata cgtccatccc cgtgggtgat
     1081 gcccgaccac agcccgctca cgacacatga taagtatacg cagcgagccg cccggaggac
     1141 aggcagcgcg ccgggcgacg ctccctcatc acccggcctc ttctcccaaa gggcgaagag
     1201 gagtccggtg tgcgtccgtt ttcccctcgc ccatcgggag aggggacaag ctccccggcg
     1261 gggagcaggg gtgagggaga gatgaccaac cacgcattcc gccgcccggc ctgcgaactc
     1321 tgttgcgcgc cgggctcctc gatacagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnctc ggagacccgc gcgccttcac ggtcatcgag tggcgaaccg gcgaggaacg
     1501 ggggcgaagc gacgttctgc gccggttcga tgtatcgaga tgaccaaccc cgcattccgc
     1561 cgcccggcct gcgaactctg ttgcgcgccg ggctcctcga tacagcgtcc gcttcgcgtc
     1621 cgccactcgg agacccgcgc gccttcacgg tcatcgagtg gcgaaccggc gaggaacggg
     1681 agcgaagcga cgttctgcgc cggttcgatg tatcgagatg accgaccacg catcccgccg
     1741 cccggcctgc gaactctgtt gcgcgccggg cacttcgaca ggctcccgcg cggcgggagc
     1801 aggggtgagg gaacaaaaaa agggcgacct gtccatgcgg ttccccgcac gcaggccacc
     1861 ctcttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca aaaaaagggc
     1981 gacctgtcca tgcggttccc cgcacgcagg ccaccctctt ctgcgcgccc ccccttactc
     2041 gagagctgcg attactccac cacgcgtacg cgtcctaccg tacgactgtc accctgctca
     2101 ccagccggct tgtcggtgtc tccatgaccg cgaagtacac cccgctggcc attgcgcggc
     2161 ctgccgcgca tattcgggtg gaaggccgcc tgtcgcactt accgaacgac tgtcaccctg
     2221 ctcaccagcc ggcttgtcgg tgtctccatg accgcgaagt acaccccgct ggccattgcg
     2281 cggcccgtgt cgtccgtgat gtcccaccgc gcctgatgcg tgccggcacc cacacggccg
     2341 ttcaccagca cccgcacctg ctgacccagc gtgttgtaca ccgccagccg caccatctgc
     2401 tcatccggca gcgaatacgc ccacaccgtc gccgggttgg ccgggttcgg gaatgcattc
     2461 cccagcgcca gccccagcgc cgcgccggct gtcacttcca ccgggtcgtg cgttttgcgc
     2521 accccgtacc ggctcacgtc ggtcaggcga tacgcatacc gcatccccgg caccacgctc
     2581 cggtccacaa actcgtagtc cgtgcggtac ggcgtcgagc ccatgccgtc gagacgatga
     2641 atctgcacaa acgtgctggc cggatcgctc acctcgcggc gttccacgat ccagtagagg
     2701 ttgtccgttt cgctctccgt tgcccacgca agcttcacca cctgccccac tgcttccgcc
     2761 gtgaacgagg cgaactccac ggggagggat cgctcgtacc ccagttcaat cgggaaattg
     2821 gcgcttgcaa ccgtgccgag gcccatcgtc acatacgagt tgttcgctag cttcgcaccg
     2881 aactgccgcg tggaaacggc tgtggtcgca acatcgtaca ctacatagaa cacacccacc
     2941 gcgcttgcgc gcaacgtgtc gccgatgccg gcagtgaacg ctgctgtgcc ggaaccgccg
     3001 ctcaatcctt ccaccagtgc cgcggcaacg cgcaggtctc ccgtcgcgct gaagctcagg
     3061 tcgccgccat cgcggtagat ctccacggag tcaatatccg ccgcatcgta ggtcccgaca
     3121 ccatacgtgt tgtcaacctt gatgctgctg atcttcaccg cgggcccgct tgtcgtgaag
     3181 ttcagccgca tgagtgcgac gttccgatcc cccggattga ctcctgaagg tgccagttcg
     3241 gcggcctttg tcaccgtaac ctgcccggtg gcgaacgttt tgctggccga ggtcaacggc
     3301 gtttccgaaa gggccgtcga caccgaaccc gaagagattg ccgtaaccgc cgcgtttgtg
     3361 gtgagttgca ggttgagcgt acgcccgctg atcccgttcg agttcgtttt cagcaccgca
     3421 agcaccgttc tgcttccgtt ctgggccagc gggatgttca agcccgtgaa cgccaccgta
     3481 ttgttcgtga acgtccgcgt atcaatcagg ttatcgttga cgtcaagctg gccgtcggta
     3541 ttgttgtcgt agtacaaccg gacctgtgcg gtctcatatc ccgcccaccc cgtgcccgtg
     3601 ttcgtaaacg tgataccggt taccgtagcc gccttgttgc cggggcccgc cagccggagc
     3661 tggatcatgg ggatgttgtc ctggttgatg gtgatctgcc cggcgctgac aggcgcgttc
     3721 cctctgctca cgcccaccac atcgttcggg tttgcgaagt tgaacacgtt cgataccgca
     3781 ctccccgtgg agtacagggt tcggcccgtg ttcgctccgg tgccctgcac ctgaccggct
     3841 tcaaccgtca ggccgagggt gttcccggct acgcccgttc cagtgttgac cgcgaccacc
     3901 acatacttga agcccccgct ggtgatctgc gtcgtcggag tggtccacga aaacgtcacc
     3961 acattgtcag ttgcactcgt ttgcgtcgca cccagttgct gagcatccgc cacaccatcg
     4021 ttgttcgcgt cctcccagag gctgacgccg gtgatctgcg tgccgaaccc tgtcgttccg
     4081 gcaaggccgc tcagcgtcac cttcaggtag ttggtcgcac cgtcaatgtt cacgttctcc
     4141 gcaaccgccc ccagctttac ctgcagcacc gaaactttcg tcgcggagtt gttcaacacc
     4201 gccgcagccg gcgtgtaagg ccccagcgcc accgtaaggt cagagaacga ccacgtggcg
     4261 gagcttgccg tgccggccga cccgatccac ttgctcgacg tgacacccct cccgacgacg
     4321 tacccggtgt cggggatgct cagcgtcaga gtccggccct gcgcgccggt gcccgtggtg
     4381 accttgacga tgaggtcacg ggagccccca ctcgcgatct gtatcgcggg cgaagtccac
     4441 gtgaaggtga caatcccgtt cgtcgcggag gtttgcgcgg aaccgagcgg cgtcgaaccg
     4501 tccgagtcga agaggctcat ggactcgatg tcctggcctg aaccgaacgt ggcggtgcca
     4561 cccagcttga tcgccacctt gttgtagctc aagtcgctct tcgagaggtc aaacgcttct
     4621 ccaaggggcg aaagtttcag gtggaaaacc tcgaatgtgg cgctgttaga gagtctgccc
     4681 gcgcccgggt tcgaggtacc gttcgaaacg ttcaacacac ccttggtgcc gtcatagaac
     4741 gtcatcgtgt tggacgtcgc cgttccttcg ctcgcccggt aataccctga cgcgtttgcc
     4801 cccacgacgt cactcgctgc cgcgatgctc aggttgaacg tcttcccgtt cgccgccgcc
     4861 gatatgtcgc tcgggatgtg ggccaccgcc acaaatcgtt ctgtagcccc tgccgtgatc
     4921 gtgaggccgg agatcgtgaa cgtgaccgta cctgcggcgg tttgcgttgt cccgcgctgt
     4981 gtttccccga cgtcgtactg cccgttgttg ttcgcgtcgc tgtagaggct tatctgcgcg
     5041 atctccgtgg gcaaagccgc cgttccgccg gccgtgagct tcacgctggt caggttcatg
     5101 tcctgcccga ccgccgttac cgcgaattga aacacgctct tcagatgcgc cccgttgaac
     5161 gtgtgcatcg tgtcggccgc cgccgtgttc gcccccagcg ccgtcgtcag atacccgggg
     5221 aatgtgaacg tgttcgaagt gagtggcggc gtaaaggtga ccgtgccacc cgtagccgtg
     5281 atgtccgtcg tcgcattgag ccccagcgtg aacgtgtgcc cggatacccc gcccgcgccg
     5341 gtccgcacga ccgcgagcat cacaacggtt tcgttctttg gaacggaaag ggtgaggctg
     5401 ctgaacgtta cggtcgtcac ggagggcgca aacgactgtg tacccagcgc gacgccatcg
     5461 ttcgcgtcga gtgtgccgtc ctggttgaga tcctgataca atcgcacttc cgcgatttcg
     5521 tcggacggca ctaccgttcc gcccgcagtc agcgtgatgc tcgatacact cgccgcggca
     5581 tcgcccggac cggcaaacgc gaactgaaag atttcgatgt cgttcgtgtt gatctgaatt
     5641 gcgccgccgg ttggtggggt gtccccgatt tcgacgccga gaactccatt tgggttggcc
     5701 acgtggaact gttcgcctgt cgccgtccca actgatgact gggattgccc ggtggtaacc
     5761 ccggtacctg ttatgtcgcc atctgcggca atactcaacc tgcacgtcac tcccgcgggg
     5821 agggcagtgt tcgcggtctt tacggtggcg accacattga cgaacccgcc ggctgttaca
     5881 aggagtggga aattcgtccc gctgaaggta accgtgctgg cggccgttgt cgttacgccg
     5941 agctgcgttt caccggtatc gaatgtcccg ctattgttcg catcaatgta gaggctcacg
     6001 ctcgcgatct ggcccggcaa ggtcgcagtt ccggacaccg tcactgtgac gccgctcaca
     6061 ttgatgtttt ccgcgcccgc ggtcagcttc gcctgaagaa gtgaatacgt tttgttcttc
     6121 gccagcagac ccgacggggg tgtattggcg ccgatttcca cacccagttt ggagaatgtt
     6181 ttggcgttcg atacagcggt gccgagggag ttgatggtgt gccccgatgt gctgcctgtg
     6241 ccgacaacgt cccccgctgc aatactcagg gtcacggtat tccccgcaac gccgtttgcc
     6301 gtggttctga cgaccgcgat aaattggatg ctgccgccgg cgggaactcg cgtttccgtt
     6361 actccactga acgtgttcgt agctcccagc tcctgctgtc ctatgagaga gtccgtacca
     6421 acatcgagaa ggccgttgtt gttcttgtct ttgtacagag taactaagtc caactcggtc
     6481 ggaacgctcg ccgttccgcc cttcgtcagc ttcacgctcg taatgttgat ttgctcaact
     6541 gctgcagaaa gcttgaaatg gaacacgccg atgtcggatg tgctcttggc catcaccccc
     6601 gcactcggcg gggatgtacc cgcggacacg gttagcacgc cgtccccact ggaaaacgtc
     6661 atggtgttag acgtggcggt tccctgagag gggcggctca gcgaagtggt cgcgccggtt
     6721 ccggagacgt ccccgttggc ggcgaggctg agggagagcg tgcgcccggc cgtggcgttg
     6781 cctgtcgccc tggcaacagc gacgatattc acgggtgtgg cggtcgtaac tgcaacgagg
     6841 ggactgccgg tgaacgtcat cgtgccggca aacgattgcg tgctgccgag aagcgtttcg
     6901 cctgcatcat agattccgtt ggcgttggcg tctccataca ggcttacctg gttgacatcc
     6961 gacgaagtga ccgaaccggc gctcgtgatc gtgatgctgt tgacgttcac cgtctcgccg
     7021 gttgccgtca ggctgaactg gaacaccgat ttcagataga ctctgtccgt cgccattgag
     7081 tcaccggccg gggtggtggc ccccagagag gcgccgagcc cccagaccgt gaacgcactg
     7141 gaggtgagcg ggaaggttcc gaccacggtt tgacccgcgg taatgtctat ggcagtcgcg
     7201 gcggcaatac cgagcgtgaa ggtgtgcccc acggtgacat tggatgtcgt tctcaccagc
     7261 accaggaagt acttcgtcgt ttttttggcg attgcccagt tgagacccga gaagttcacc
     7321 gtattgttcc cgctgacaaa cgtctgcgcc gttcccagag gcacgtccac accgacgtcg
     7381 aacgccccgt ttgcgttcgt gtcctcgtag agcttgacct ggtccacctc ggtaggtaca
     7441 accgccgttc cgccggcgct gaatcgtatg gagttcaccg tgattgcagt gttgttggcc
     7501 gttttcacac tgccacggaa cacaaaaatc tcgctcgtat tcgcgggcaa ctccccggcg
     7561 gcgggtgacc cggccagcgc aaacgtgtgg ttggcgcccc acgcctcacc agtcgcgaac
     7621 atcacgacgg cacccagaat cgtcagcagt tttttcatgt gtctctccgc cccccgggct
     7681 tgccgtgcaa caccacacgg ccgcgcgaga tatgtgaaca taaatcgttt gattgcaagt
     7741 cgttcgctgc ttcacccacc caccgtatgt gacgtatttc acacaggatg ggaaccgtca
     7801 aagactaaca cggcacacta caataaacca cgcgggcaaa caattgtcaa tactaacaac
     7861 gaaatttact gcaattctag aatgccaaaa acgttacact cgtcttctcc cgcaagttcc
     7921 aacctccggt acgtggaggt ttccggcatc accttttccc ggatggcaac caaaaaaaac
     7981 ctgccccgcc aaccggtcct cgagttgcga acggagcgaa gcggaccttc ccggccatcc
     8041 ccagaacgac ccccggcacc ctgcgcggtc cctgagcctg ccgcccgggc gggcagcctg
     8101 tcgaagggcc agcgcaggcc ttccgccgcg tccacccagt ggagggacac gctgagaacg
     8161 cccggcgttc cactcctcgt gtccgtcctc ccgccaccgt ggagggacac gcttcgtcgt
     8221 gtctaacgga aacgcggggc tcctcgatac aaccccggga acgcgcaacg ttccccggaa
     8281 cgaaagcaag gcaacgttcc cggtgtcact cggagacccg cgccggacgg gcggtggcgc
     8341 agtacggtcg tggagcggcg aacccgcccc ctgagcctgc cgcccgtccc ggcaccccgc
     8401 gccggtccct gagcgtgctc ccgcgatggg cgggagcctg tcgaagggcc cttccacaca
     8461 gtccacccct tggaggcaaa tgctgagaac gtctggcgtc ctactcgttg tttccgtcct
     8521 cccgccaccg tggagggaca cgcttcgtcg tgtctaacgg aaacgcg
//
LOCUS       DAOSOO010000139         8556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8207_length_8556_cov_6.575109, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000139 DAOSOO010000000
VERSION     DAOSOO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_8556_cov_6.575109"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLT86_10000"
     CDS             complement(<1..257)
                     /locus_tag="PLT86_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36984.1"
                     /translation="MTRRSGFRVRGAHDAFAGMTVAVEGASGPVCSAPDPDAGRVGRM
                     GPMGRMGPVRADTGVRPYLSIALRRPSHRCRSARTGWRRSIR"
     gene            complement(569..1168)
                     /locus_tag="PLT86_10005"
     CDS             complement(569..1168)
                     /locus_tag="PLT86_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT36985.1"
                     /translation="MPRITTILFDLGNTLLWFDFKPVFTRLLSMSKGDPERITSAVNA
                     RYDDFAAGRMTPAEWHEFIQRTCGISMPWEEFSRLWADIFWEHPEMFALARETRPRYR
                     QYLLSNTDAIHFPWCCERFPLEEVLDGYILSYELGAVKPQREIYERGLAKFGLKAEEC
                     VFIDDLEANLEGARGVGIHTVRCVSIEQVRRDLRELGVG"
     gene            complement(1181..2053)
                     /locus_tag="PLT86_10010"
     CDS             complement(1181..2053)
                     /locus_tag="PLT86_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT36986.1"
                     /translation="MTDGLPRPAPGQPVTASMTCGGLTFAGASESAHTTFVFCRQLDL
                     IFDIGSVVDAMLPIDTVLISHTHQDHLLGLTRYICLRRLQGMKPPRVLLPEASVEPVR
                     RLLAVWQELEGGADRIPPDVALEPVEAGQEVRLSGNLVARAFPVAHVLPSLGYTVITR
                     IHKLRREFYGRSGQELARLREEGVAITDPHDTPLVTFIGDTLPETLERTPDIGTSPVV
                     ILECTFLTPEHADLATPRGHLHIRDIIERIALFGNAEIILTHFSRRYRSDKVRELVQQ
                     ALPPELAPRFHVLA"
     gene            complement(2050..3150)
                     /locus_tag="PLT86_10015"
     CDS             complement(2050..3150)
                     /locus_tag="PLT86_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36987.1"
                     /translation="MNVFAHPMPEVYGDCADIRAITHDRTQSQKWISTTSTLFNPNDG
                     LTYVGLTHLGGDLLWAYDPATGKSRSCGYLEIRGENEVKIHRSLELGPDGRIYGATAG
                     LTDVKARLTSPGGQIWAYDPRTGVFESFGIPVPNDYVQHVTFDWKRRMAYGCTYPVPW
                     FFAYDLEARETVFSAFTGALPHRSAVDRNGRVWSGYTNTADISGENFLFFYDPDANAL
                     TWTDLHIPKCGQADKMQVDDMVALADGFVYVASVDGVLARLDPDAHEIEWLGKPARGM
                     RMCGICEGPDGLVYCLTGAFYGMPKEDRPTRVFAFDRSTKQFRELGQIFDPEFGDGCA
                     VVHHLSIAPDGTLWVGETDNDQRSGCLWECRP"
     gene            complement(3196..3996)
                     /locus_tag="PLT86_10020"
     CDS             complement(3196..3996)
                     /locus_tag="PLT86_10020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT36988.1"
                     /translation="MTHIEAASNAARAGAEVLLRHFRGLQTDDIRTKARNDFVSFVDR
                     ESETAIRGVILDAFPGHEFLGEEHGSVGGSEWRWIVDPLDGTSNYVHGVPQFAISIAL
                     QHGGTLVAGLVFDPVRGDEYVAERGAGAWRNGERLRVADGQPLEDALIGTGFPFRARH
                     VFDDYMATFREIYPRVSDVRRPGSAALDFAFVAAGATGGFWEFALSPWDIAAGALLIE
                     EAGGVITDFEGGSAYLSSGNVVTGAPRIHRELLACVQKVLRERGSALA"
     gene            4134..6467
                     /locus_tag="PLT86_10025"
     CDS             4134..6467
                     /locus_tag="PLT86_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT36989.1"
                     /translation="MLYLRPPIDRPLTRTEYKRGMRDMLLLGAGLMPYGTIAGVSSAI
                     FAGYVLHLGVDKAAIGLILSLVQATGLLQLVSFRLSDRVHPRALVLGLGSLEVLFGVS
                     VITIPFWVPRGWEFYAVVGLLGIAWTLANTYAPTFNAWFASIVPSDMRGRYISRRTFV
                     QYGVGIAVSLLAGKFIDLVPGMKGFGILYAVALVSGLLAFSVLYRTPLPVLGQSEEDS
                     ARGESHWRRMLAPLRHREFRSFVTFHVVWTLIAAMPGPYYTVFMIDSLKLSYSTIAIF
                     TSTQMVIMGLGFRFWGAVVDRFGGKPMVQLLFIPAAAMPALWAFATPGMYYTVPVAMF
                     FGGLFWAGLSISTSIMLYAIIPQNGQKSSYFAIWSVSVGLAASAAPAVGSAVMKALTD
                     VHFVWGGMVIDNYHTLFVLATAASVIPALLIRTLPDLGAQRPGYVISQLMRGNPFLLA
                     YNIFLLARSDSARKRANLLRALGRSHNPLAVKRIVEALSDPVPEVRRAAALALGECNQ
                     AEAVEALKKHLANPESDIKVEAARALGTLEERTAIPLLIEALWSDRLDLCVAAAEALG
                     RMDDKSARDALVSRLESGFDRTLSPAIIEALSFPVGDGPLGDLRAVRPAVAMMSSFSS
                     EVIRVQLLNAIARCLGEGDRFLTLNLEDPYERDEHLSELIIETRDALFSLPWPAEEEL
                     TVAITFERMALARSEVRVSAYIQTAARLAELLRKHFALVEPGSEPTTVEEACAALISL
                     EANGQLESLQERGMTFSVLLLHRAATQTRNEIDHRRP"
     gene            complement(6481..>8556)
                     /locus_tag="PLT86_10030"
     CDS             complement(6481..>8556)
                     /locus_tag="PLT86_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36990.1"
                     /translation="NVLAIGCSLAYDEMRKRIRTEISGTGDNVRSYVRLCQMSFGWDF
                     APRLVVIGPWRPVNLICHKRAAIETIFVNTRAIAAGSADLSIEVRTRSVRRMESAPEL
                     HLSIHENEGTPPVWEHTCPIHGDGRITVEVHLPSARLWYPAPLGEPFRYYVKATVSCD
                     GEIVSRKTARFGIRSVELKQNGQFTFCINGVDVFARGANWVPSNSLTLDSTPERYRHL
                     LELAHEAHFNMLRVWGGGIYEPEVFYDLCDELGIMVWQDFMYACAMYPDDDTAFMENA
                     TREARQVVTRLRCHPSVVLWCGNNECQEAWTLGDWPDRAPRHFGERLYDHVLPDTVRE
                     LSPGIPYWPGSPYGGPTTRSREVGDFHDWYSFPNWRDYDKNAPLFSSEYGFRSVPARE
                     TVDEMISDELQWDGNSPLSRPGSLHKVWQFHHGWCGWLQSILPQFGDPTSLDQFIMLT
                     QEAQATLMRYAVEVYRRRMFKTSGSLIWQYDDPWPAVTFSLVDHFGRPKAAYFWVKEA
                     HAPVIGMFYEKDGATGYWGISDLQNTQACSLRIRRFTHSGALLGDATFGGALLPNTAT
                     CIVENLPQNLTIASPDEEYLYAELVAGGMLSERVYHVGKRCDWRLPAATIEASAAREN
                     ETRVRVKLTSSGYVHFVCVSVADPAARYSTNAVDLLPGEQREIVIRTRERGPITIHSA
                     NAPTLVVEV"
BASE COUNT         1541 a         2788 c         2581 g         1646 t
ORIGIN      
        1 cggatgctcc tgcgccaccc cgtccttgcg ctcctgcacc tgtgggatgg ccgccgcaag
       61 gcgattgaga ggtaggggcg gacccccgtg tccgcccgaa ccggccccat ccgtcccatc
      121 ggacccatac gtcctacgcg cccggcgtcc gggtcgggcg cgctgcacac cgggccggac
      181 gccccctcca ctgcaaccgt cattcccgcg aaggcgtcgt gcgcgccgcg cacgcggaat
      241 ccactccgcc gcgtcatgga ggggcacgct ccgtcgtgcc cttgcccccg cgaagaacgg
      301 aaccgtcgga cgcgacaaag cgcgtccctc cagagtcgtt ctcgcagcca tccccacgcc
      361 ggccttcgtt gccgccatcg ccgcgacggc tccttgcgcg cgccacaaca accgtcattc
      421 ctgcgcaccc ttcgtacgcg gcgcgcacgt acaccttcgt gtccgcccca agcggcccca
      481 tccgtcccat aagacctata cgtcctacgc gcccggcgtc ccggtcgggc gcgttgcaca
      541 ccgggccgga tgcccctcgt ccccccccct accccacccc caactccctc aaatccctcc
      601 tgacctgctc aatcgacacg caccgcaccg tatgaatccc cactccgcgc gcaccttcaa
      661 ggttcgcctc cagatcatca atgaacacgc actcctccgc cttgaggccg aacttggcca
      721 ggccgcgctc gtaaatctcc cgctgcggct tcaccgcgcc gagttcgtac gagagaatgt
      781 acccatcgag cacctcttcg agcgggaacc gctcgcaaca ccacgggaag tgaatcgcgt
      841 cagtgttcga aagcaggtac tgccggtagc gcggccgcgt ctcccgggcg agggcgaaca
      901 tctccgggtg ttcccagaaa atgtccgccc agaggcgcga gaactcctcc cacggcatgg
      961 atattccgca cgtgcgttgt atgaactcgt gccattctgc gggcgtcatc cgccccgccg
     1021 cgaagtcgtc gtagcgcgcg ttcaccgcgg acgtgatgcg ctccgggtcc cctttcgaca
     1081 ttgacagcaa ccgggtgaaa accggcttga agtcgaacca gagcagagtg tttccaagat
     1141 cgaaaagaat cgtcgtgatg cggggcatac ggtctccgtg ctacgcgaga acatgaaacc
     1201 ggggtgcgag ttcgggcgga agcgcctgct ggacgagctc ccgaactttg tcgctgcgat
     1261 accgccggct gaagtgggtc aggatgattt ccgcattgcc gaacagcgcg atgcgttcta
     1321 tgatgtcgcg gatgtgcagg tgcccccgcg gcgtggcgag gtcagcgtgt tccggcgtaa
     1381 ggaaggtgca ctcaagaatc accaccgggc tcgtgccgat atccggcgtg cgctccagcg
     1441 tctccggcag cgtatcgccg atgaacgtca ccagcggcgt atcgtgcgga tccgtgatgg
     1501 caaccccctc ctcccgcaac cgggcaagct cctgtcccga ccggccgtag aactcacgcc
     1561 gcagcttgtg gatgcgcgtg atcaccgtgt accccagcga cggcaggacg tgggccaccg
     1621 ggaaggcgcg cgccacaagg ttgccggaaa gcctcacctc ctgccccgcc tccaccggtt
     1681 caagggcgac atcgggcgga atgcggtccg ctcccccctc cagttcctgc catacggcaa
     1741 gcagacgccg caccggttcc accgaggctt ccggaagcaa cacgcgcgga ggcttcatcc
     1801 cctgaaggcg ccgaaggcag atataccgcg tgaggccgag cagatggtcc tgatgcgtgt
     1861 gcgagatcaa caccgtatca atcggcagca tcgcgtccac tacgctgccg atgtcgaaga
     1921 tcaggtcaag ctgcctgcag aacacaaacg tcgtgtgcgc cgattccgat gcgcccgcga
     1981 aggtgagccc gccgcacgtc atactggcgg tcacaggttg cccgggagcc gggcgcggga
     2041 gcccgtccgt cacgggcggc attcccacag acagcccgaa cgctggtcgt tgtccgtctc
     2101 ccccacccag agcgtgccgt ccggcgcgat ggaaaggtgg tgaacgaccg cgcatccgtc
     2161 accgaactcg gggtcgaaaa tctgccccag ttcgcggaac tgcttcgttg aacggtcaaa
     2221 cgcgaacaca cgcgtcggcc ggtcctcttt gggcatgccg tagaacgcgc cggtaaggca
     2281 gtacacaagc ccgtccggcc cttcgcatat gccgcacatg cgcatgcccc gggccggttt
     2341 gccgagccat tcaatttcgt gcgcgtccgg gtcaagccgg gccagcacgc catccacgct
     2401 ggccacgtag acaaacccgt ccgcaagcgc aaccatgtca tccacctgca tcttgtccgc
     2461 ctgtccgcac ttcggaatgt gcaggtccgt ccaggtgagc gcgttcgcgt cggggtcgta
     2521 gaaaaagagg aagttctcgc cggatatatc cgccgtattg gtgtacccag accacacccg
     2581 tccgttgcgg tccaccgcgg accggtgcgg cagcgccccg gtgaacgcgg aaaacaccgt
     2641 ttctcgcgcc tcgagatcat acgcgaaaaa ccaggggacg gggtacgtgc agccatacgc
     2701 catgcggcgc ttccagtcga acgtcacgtg ctggacatag tcattcggaa ccggtatccc
     2761 gaacgactcg aaaacgccgg tgcggggatc atacgcccat atctgcccgc cgggcgacgt
     2821 gagacgggct ttcacatcgg taaggcctgc ggtcgctcca taaatccgcc cgtccgggcc
     2881 aagctcaaga gagcggtgaa tctttacctc attctctccc cggatctcca gatacccgca
     2941 ggaacgcgat ttccccgtgg ccgggtcgta cgcccagagc agatccccgc ccagatgggt
     3001 gagcccgacg tacgtcaggc cgtcgttcgg gttgaacagc gtgctcgtcg tggaaatcca
     3061 cttctgcgat tgcgtcctgt cgtgcgtgat ggcgcgtata tcggcgcagt cgccgtacac
     3121 ctccggcatc gggtgcgcga acacgttcat tgcctgttct cctttgtcgg tcctgattct
     3181 gttcggcgcc cggcactatg cgagcgcact gcctcgctcc ctcaacacct tctgcacaca
     3241 ggcgagcaat tcccggtgga tacgcggcgc gcccgtcacc acgttccccg atgaaagata
     3301 cgcgctgcct ccctcgaaat ccgtgatcac gccgcccgct tcttcgatga ggagcgcccc
     3361 cgccgcaatg tcccagggcg aaagtgcaaa ctcccagaaa ccacccgtgg caccggccgc
     3421 cacgaacgcg aaatcgaggg cggcggaacc cggccgtctg acgtcggaaa cgcggggata
     3481 gatttcccgg aatgtagcca tgtaatcgtc aaacacgtgg cgggctcgga acggaaaccc
     3541 ggtgccgatc aacgcgtctt ccagcggctg cccgtccgcc acgcgaaggc gttctccatt
     3601 tcgccacgcg ccagcacccc gctcggcaac atattcatcg ccgcgaaccg ggtcgaacac
     3661 gaggcccgca acaagagttc cgccgtgctg cagggcgatg gaaatggcaa attgaggaac
     3721 gccgtgcacg taattgctgg tcccgtcgag cggatcgaca atccaccgcc actcgctgcc
     3781 tcccacggaa ccgtgctctt ccccgaggaa ttcgtggccg ggaaacgcgt cgagaatcac
     3841 gcccctgatg gccgtttccg actcccggtc cacgaacgaa acgaagtcat ttcgcgcctt
     3901 tgtgcggatg tcatcggtct gaagcccgcg gaaatgcctg agaagcacct cggctcctgc
     3961 ccgggccgcg ttggatgctg cttcgatgtg tgtcagactt acccctgtcc cggagatggc
     4021 ctccgttatc gctggtgtgg tgcattcctt ccgaaagccc gtacatgggc tctatattta
     4081 gaaggcaaat cgtcgtcctc gtgtcaatac ctccacgaaa aaaaagaaga gctttgcttt
     4141 accttcggcc tcctatcgac cggccgctta cgcgcaccga gtacaagcgc ggcatgcgcg
     4201 atatgctgct cctcggcgcc ggcctgatgc cctacggtac catcgcgggc gtctcgagcg
     4261 ccatttttgc cggatacgtg ctccacctcg gcgtcgacaa agccgcaata ggacttatcc
     4321 tttccctcgt ccaggcaacc gggctcctcc agctcgtaag tttccgcctc agcgaccgag
     4381 ttcacccgcg cgcactcgtg ctcggcctcg gttcgctgga ggtccttttc ggcgtgtccg
     4441 tgatcaccat ccccttctgg gttccccggg ggtgggagtt ctacgcggtg gtcggcctcc
     4501 tcggcatcgc gtggactctg gcaaacacgt acgcacccac cttcaatgcc tggttcgcca
     4561 gtatcgtgcc atcagatatg cgcgggcgct acatcagccg gcgcacgttc gttcagtacg
     4621 gcgtaggaat cgccgtctct ctgctggcgg ggaagttcat cgaccttgtc cccggaatga
     4681 agggcttcgg aatcctgtac gccgtggcgc ttgtctccgg gttgcttgcg ttctcggtgc
     4741 tgtatcgcac accgttgccc gtcctgggcc agtctgagga ggattccgca agaggcgagt
     4801 cgcactggcg gcgtatgctc gcccccctgc gccacagaga attccgctcc ttcgtcacct
     4861 tccacgtagt ctggacactc atcgccgcca tgccgggacc ctattacacg gtcttcatga
     4921 tcgacagcct gaagctttcg tactccacaa tcgccatctt cacctccacg cagatggtga
     4981 tcatggggct cggatttcgc ttctggggag cagtggtgga tagattcggc ggaaagccga
     5041 tggtccagct cctgttcatt ccggccgctg caatgccggc tctctgggca tttgccaccc
     5101 cagggatgta ctacaccgtt cctgtggcga tgttcttcgg cgggcttttc tgggcgggtc
     5161 tctccatctc cacgtccatc atgttgtacg cgatcatacc tcagaacggg caaaagagct
     5221 cctacttcgc gatctggtcc gtgtccgtag ggttggcggc ttccgcggcg ccggcggtcg
     5281 gcagcgcggt aatgaaggcg ctgacagacg tgcatttcgt atggggcggc atggtaattg
     5341 ataattacca tacgctcttc gttctggcga ctgccgcgag tgtgatccca gcgctcctca
     5401 tcaggaccct gcccgacctc ggcgcgcaac ggcccggata cgtaatcagc cagttgatgc
     5461 gcggaaaccc gtttcttctt gcgtacaaca tctttctcct tgcacggtcg gattccgcgc
     5521 ggaagcgggc gaatctgctg cgggcactgg gccgctccca caacccgctg gcggtgaaac
     5581 gcattgtgga ggcgctgagt gacccggtgc cggaagtccg cagggcagcg gcacttgcgc
     5641 tgggcgaatg caaccaggcg gaggcggtcg aagcgctgaa gaagcacctt gccaacccgg
     5701 aatccgacat taaggtggaa gccgcgcggg cgctgggaac gctggaagag cgaacggcta
     5761 tccctctgct tatcgaagcg ctctggtccg accggctgga cctgtgtgtt gcggcggcgg
     5821 aagcgctggg caggatggac gacaaaagcg cccgggatgc gctcgtgtcg agactggagt
     5881 ccggttttga ccgcaccctg tcgcccgcga ttattgaagc cctgagtttc ccggtgggcg
     5941 atgggccgct gggggatctt cgggcggtgc ggccggcggt tgcgatgatg agttcgtttt
     6001 cgtccgaggt gatacgggtt cagcttctga acgcgatcgc gcggtgcctc ggagaaggcg
     6061 accggttcct cacgttgaat ctggaggatc cgtacgaacg ggatgagcac ctttcagagc
     6121 tcatcatcga aacgcgcgac gcgcttttca gtcttccctg gccggccgag gaggaattga
     6181 ccgtcgcgat caccttcgag aggatggcac tggcgcggtc ggaggtgcgg gtttccgcct
     6241 acatccagac cgcggcgcgg ctggcggaac ttctccggaa gcattttgcc cttgtcgaac
     6301 ccggcagcga accgacgacc gtggaggagg cgtgcgccgc gctgatatcc ctcgaggcga
     6361 acgggcagct tgaatcgttg caggagcggg gaatgacgtt ttccgttctg ctcttgcacc
     6421 gcgcggcgac gcagacgcgc aacgaaattg accacagaag gccctgatac cgggccccgg
     6481 ctatacctca acgacgaggg tgggggcgtt ggcgctgtga atggtgattg ggccgcgctc
     6541 ccgcgtccgg atcacgattt cccgctgctc tcccggcaga agatccaccg cattcgtgga
     6601 ataacgggcc gcggggtcgg cgacactgac gcagacgaag tgcacgtacc ccgaagaggt
     6661 gagtttcacc cgaaccctcg tttcgttctc tcgcgccgca cttgcttcga tggtggccgc
     6721 ggggagccgc caatcacacc gctttcccac gtgatagacg cgttctgaga gcattcctcc
     6781 cgcgacgagt tcggcgtaga gatactcctc gtccggcgac gctatcgtaa gattctgcgg
     6841 caggttctcg acgatgcagg tcgccgtgtt cggcaggagt gctcctccga atgtagcgtc
     6901 tcccaggagc gccccggaat gggtgaagcg ccttatgcgg aggctgcatg cctgcgtatt
     6961 ctgcagatcg ctgattcccc agtatcccgt tgcgccgtct ttctcgtaga acatgccgat
     7021 gaccggtgcg tgggcttctt tcacccagaa ataggcggct ttgggtcgtc cgaaatgatc
     7081 aacgaggctg aaggtgacgg cgggccaggg gtcgtcgtat tgccagatga gcgatccgct
     7141 ggttttgaac atgcgtcgac ggtacacctc gaccgcgtag cgcatgaggg ttgcctgagc
     7201 ttcctgggta agcatgatga actgatcgag gctggttgga tcgccgaact gcggcagaat
     7261 ggactgcagc cacccacacc agccgtgatg gaactgccag actttgtgaa gtgatccggg
     7321 gcggctcagc gggctgttgc catcccactg gagttcgtcg cttatcatct cgtccactgt
     7381 ttcgcgagcg ggaacagaac ggaatccgta ttcgctgctg aacagcggcg cgtttttgtc
     7441 gtaatccctc cagttgggga agctgtacca atcgtggaag tcgcccactt cgcgggaacg
     7501 cgtggtcggg ccgccgtacg ggctgccggg ccagtacgga atgccggggg acagttcgcg
     7561 cactgtatcc ggcaacacat ggtcgtacag ccgctcgccg aaatgccgtg gggctctgtc
     7621 aggccaatcg ccgagcgtcc acgcttcctg gcattcgttg ttcccgcacc agaggaccac
     7681 cgacgggtgg caccgtaatc gggttacgac ctgtctcgct tcgcgcgtgg cgttctccat
     7741 gaacgccgtg tcgtcgtcag ggtacatagc gcaggcgtac atgaaatcct gccacaccat
     7801 gatgccgagt tcatcacaga ggtcgtagaa cacttccggt tcgtagatgc ctccacccca
     7861 cacgcgcagc atgttgaaat gcgcctcgtg agccagttcg agcaggtggc ggtagcgttc
     7921 gggagtggag tcgagcgtaa ggctgttgga gggaacccag ttggcgccgc gggcgaacac
     7981 gtcaaccccg ttgatgcaga aggtgaattg tccgttctgc ttcaattcca ccgatcgtat
     8041 cccgaagcgt gccgttttcc gcgagacaat ttccccgtcg cacgacacag tggctttgac
     8101 gtagtaccgg aagggctctc ccaacggagc ggggtaccac agcctcgccg acgggaggtg
     8161 tacttccacg gtgatcctgc cgtcgccatg aatggggcag gtgtgctccc agacgggggg
     8221 tgtgccttcg ttttcgtgga ttgaaagatg gagttccggc gctgattcca tccgccggac
     8281 ggatcgggtc cgcacctcga ttgagaggtc ggcactgccg gctgcgatgg cccgggtatt
     8341 cacgaagatg gtttcgatgg cggcacgttt gtggcagatg aggttgaccg gcctccatgg
     8401 tcctattacc accagccgcg gcgcaaaatc ccacccgaag ctcatctggc acagacggac
     8461 ataggaacgg acattgtcgc ccgttccgct gatctccgtc cggattcgtt tgcgcatctc
     8521 gtcgtatgcg agggaacacc cgattgcgag cacgtt
//
LOCUS       DAOSOO010000140         8526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8242_length_8526_cov_5.749852, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000140 DAOSOO010000000
VERSION     DAOSOO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8242_length_8526_cov_5.749852"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3610
                     /locus_tag="PLT86_10035"
     CDS             <1..3610
                     /locus_tag="PLT86_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT36991.1"
                     /translation="AILVTAAAARSQALPDTSLMTIYGNPGHYGVAAHPDGVHAIAAS
                     TDGSVRLWNIVTGALERILTEHRGIAYAVAVSSDGGMIVSGGMDSTIRVWSFADGSLL
                     RTIPTGAVVRSLALTPDGQKIVSGGWDAAVKVWDTSTGSLLHTLTGHTKQVYSVAVSA
                     DGMTAVSGSDDKTARVWDLVNGQLVRTVTGHFYGVWAVAINPQGTIGISATSNGTMKT
                     WDTATGATLDSIPAGGTNHRGAVWSIAVTPDGLSFVSGSSDQTIRVWELATGALLRTL
                     PVGSDAGHTDQVYGIALTQDGSTILSASADRSVKAWRLSDGALLQTLSSPAGRHRGSV
                     DAVYLSPDGNTLISGSADSTIKMWNLADGALLRTLPVGSDVGHVGTVWAFEMTSDGST
                     LISGGDDGTIKLWNAADGTLTRTLPVGADAGHEYAVYSLALSPDGQTLASGSGDLTIK
                     LWQMSDGALLKTLPVSGGYVDNRCVWAVAFGAGGQELVSAGGEGFLKVWQVSDGALLR
                     TLPLPGDEGHSGDVWSVTVAPDGQTIVSGGLDGAIKLWNYATGSLIRTITAGGATGHT
                     QPIWRVIVTQDGRNIISASDDRTIKVWDLATGALLRTLTGHTGPVWGLAISADGLKLA
                     SSGSSDMTIRTWRLAAPQQTETFVSGTITTQTWYKANSPYRVTGTITVPAGNTLTIEP
                     GVDVLFNADVQFIVQGSLHAVGTPQDSIRFVKGTATEWGGLRISGGDSSTIAYARVSN
                     GRADGNVPDVFGGGIYVAGAGTRLGMRSVVVSGNISNQRGGGVAVRDGAQVTMDRCLI
                     SKNWAIDYGGGGLDVENGAVLTMNRSTVTGNTGTPGTAILLNTATWNVTNSIIWGVYF
                     PLATPTFSASYSDFQSVYEGTGNINADPLFADAANGDFRLRPGSPCIDAGDPTSPPDA
                     DGTVADMGALPRMFHIGDVTRDDAFTALDASWVLQYSVKLRESLPLSLADVSGNGRAS
                     AYDAGLILHKAIIDLSFVFPAGDIAGGEKVGRGTPRSMGFVRADDGWNLVVSNPDGVL
                     GCDLTLLLPETAEATFSGEGAVEHARDGGTVLVGIARTEFTNPVLLHVSGTTDPPQIV
                     RASLNEGAIPAVVNAPLAFSLSQNTPNPFNPSTTIRFSLPEAGPVTLAVYDVNGRLVR
                     NLVGVAASAAQTFLSGQHSVVWDGRDDNGREVASGVYLARLTAAHGVLTRRMVLVR"
     gene            complement(3836..4081)
                     /locus_tag="PLT86_10040"
     CDS             complement(3836..4081)
                     /locus_tag="PLT86_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36992.1"
                     /translation="MEDYPPEVARRLSRLRWTAALLWIGWTIVAVPIYLAYRYYPMLF
                     GVPHLKIGAITLLSLGAVWFIYLLYRIGEIARGAGKG"
     gene            complement(4084..4434)
                     /gene="ytxJ"
                     /locus_tag="PLT86_10045"
     CDS             complement(4084..4434)
                     /gene="ytxJ"
                     /locus_tag="PLT86_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HOT36993.1"
                     /translation="MVDITSVEQAEALISGAKERPLIVFKHSTRCPVSGRAAGEVEAF
                     SRTPEAAGVTLARVLVVENRPVSLWLAERLQTPHASPQALLVADGKVVWTASHFGVTA
                     DAMASALRGLAGKG"
     gene            4667..6850
                     /locus_tag="PLT86_10050"
     CDS             4667..6850
                     /locus_tag="PLT86_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT36994.1"
                     /translation="MTRSLPVFLSRAGDRVSLCLTAIALAGMFLLAGCRVTDRPAVTV
                     TTVDAQNGVRIASMPAVMVGGFGVGAGQVQSVGPVAVTEDGQLYIADNTQRRITVISA
                     ETGPLPTRSYLPGLANPLVPGDTVHVVRAMEWHGPTLYLVDRARIWAIPRSAGPGAQA
                     SAIEPEGGVADVRDIEVAADGSLYLLSERGIRILSPSGGLRDSILVRGETPPRCVAFS
                     FGPDSNLYISTRSLNHVVVYNRSGQLVKRIGAGTFAGEARGIAVDQFGNVFVRDEKQA
                     AIRAFSADGTYRGKIGARGSGPGQMLSAEQLVIDRGRRALVVPDPTNYRVQVFDLTGR
                     RAEARDLVTHTPAFERTRRPYHITLGMGVDASTQLQIAWKTAPASTGSMVVYVPVDGG
                     DISAVDWSEPGVKTARGSSTAFVSNIGPYRAHSVALTALEPGTRYAYRVGDGSSEGWS
                     ETNTFLTPPASPESLVVAILGDSRNRMDVWGNIVNRAAAFDPAFIVTTGDLVSNGEDF
                     THWDPWFHEADAVFKKTPFMTTPGNHERESPNYYLSFEMPRNAPAHLQEKCFSFDVGQ
                     THWISLNTQVDLAPQTAWLEEDLRKNTKPWVFVFLHRPAYAAHASRGDGNRDVREAWC
                     GLFEQYGVDVVWQGHDHYYHRSKPVRAANVVPEGRGPIYITTGGAGAPLYQMKPNQYT
                     EVGEITDHFCMMTLRGNHCEVVVYRADGSVADRFSVRDCPRETAR"
     gene            6834..7793
                     /locus_tag="PLT86_10055"
     CDS             6834..7793
                     /locus_tag="PLT86_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOT36995.1"
                     /translation="MKLRDEVAVEHTIAGWALIPTRQETFDPAWREVRPAGAVWESQR
                     VPVPHGNEAILSWNVPPEVQPGLAFEMSGQVRGRWSGWTRVGEWGEAPAPGVDDPLLR
                     REVDTVVWDAHVDELRVRVRTATGEHLKLDRLVLAFPAAAPKESIALPTTFNQELPFR
                     SQRTEDESIGDRICGATCVAMALAPRVPNVTAEAIAKAAYDPVHDIYGNWARLAAAAS
                     QHGAVAWVQRLASLDDVFARLVAGYHIICSVAFENGKLTGAPVQNTSGHLVLIRGWNK
                     KGDVVCNDPAFTDSRGDGVVYRRSEFERLWLTHGGAAIILRWE"
     gene            complement(7799..>8526)
                     /locus_tag="PLT86_10060"
     CDS             complement(7799..>8526)
                     /locus_tag="PLT86_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36996.1"
                     /translation="LASIADFGVDGFTLSMLAGGAAGLYAPLEWSKTRPVSDARASLI
                     SFAGSWGMWQGIGWTNALVDEPSADAAITAGLLTGGAGILATAALTGQHRISSGDATF
                     ITTAPAWSSAYWLWVMVLTGTENGRFVLGTTLAAGDIGVATGVLLADRIEASQGRVRL
                     ANLGGIVGGLVGGAAVALTNVDSPKVGVSIVMVAATLGLGGGWYLTRNYDRVDAAVSR
                     VQVAPLLVAGNHPVRGLQAAYSF"
BASE COUNT         1542 a         2662 c         2842 g         1480 t
ORIGIN      
        1 cgcaatcctt gttaccgccg ccgcggcgcg ttcgcaggcg ctgccggata cgtcactcat
       61 gacgatctac ggcaaccccg ggcactatgg tgtggcggcg cacccggacg gcgttcacgc
      121 aatcgccgcg agcacggacg gttccgtgcg cctctggaac atcgtgaccg gagcgctcga
      181 gcgcatcctc accgaacacc gcggaatagc gtatgcagtc gcagtcagct ccgacggcgg
      241 gatgatcgtc tccgggggca tggactccac gatcagggtc tggtcgttcg cggacggatc
      301 actcctgcgg accatcccca cgggcgccgt ggtgcggagc ctcgccctta cgcccgacgg
      361 gcagaaaatc gtctccggcg ggtgggacgc cgcggtcaag gtctgggaca catccaccgg
      421 ctcgctcctc cacacgctca ccggccacac gaaacaggtc tacagcgtcg cggtcagcgc
      481 ggacgggatg accgccgtat cgggcagcga cgacaaaacc gcgagggtgt gggacctcgt
      541 caacggccag cttgtccgca cggtgacggg tcacttctac ggcgtgtggg cggtggcgat
      601 caacccgcag ggcacaatcg gcatttcggc gacctcgaac ggaacgatga aaacctggga
      661 caccgcaacc ggggcgacgc tggattccat tccggcgggc ggaaccaacc acaggggcgc
      721 ggtctggagc attgccgtca cgccggacgg tctgagcttc gtgtcgggaa gctcggatca
      781 gaccatcaga gtgtgggagc ttgcgacagg ggcgctgctg cgcacgctcc cggtcggttc
      841 ggacgcggga cacacggacc aggtgtacgg gatcgcgctg acacaggacg gaagcaccat
      901 cctttccgcg agcgccgaca ggagcgtgaa ggcgtggcgt ttgtccgatg gtgcgctgct
      961 gcagacgtta agttcgcccg cgggccggca cagaggatca gtagacgccg tatacctgag
     1021 cccggacggg aatacgctga tatcagggag cgccgacagc acgatcaaga tgtggaacct
     1081 cgccgatgga gcgctcctgc ggacgcttcc cgtggggtct gacgtcgggc acgtgggcac
     1141 ggtatgggcg ttcgagatga cgtccgacgg cagcaccctc atttcggggg gcgacgacgg
     1201 caccatcaag ctctggaatg ccgcagacgg cacgcttacc cgcaccttgc cggtgggagc
     1261 cgatgcaggg cacgagtacg cggtgtactc gcttgccctg agccccgacg gacagacgct
     1321 cgcctcgggt tccggggacc tgacgatcaa gctctggcag atgtctgacg gcgcgctcct
     1381 gaaaacgtta cccgtcagcg ggggatatgt cgataacaga tgcgtctggg ccgtcgcgtt
     1441 cggcgcgggc gggcaggagc tggtgtcggc aggcggagaa ggattcctca aggtctggca
     1501 ggtctccgat ggcgcattgc ttcgcacgtt gccgctgccg ggggatgagg ggcactcggg
     1561 cgatgtctgg agcgtgacgg tggcgccgga cggccagact atcgtctccg gcgggctgga
     1621 cggggcgatc aagctgtgga actacgccac cggctcgctc atacggacga tcacggcagg
     1681 cggggctacc ggccacacgc agccgatctg gcgcgtgatc gtcacgcagg acgggcggaa
     1741 catcatctcc gccagcgacg accgcacgat caaagtgtgg gaccttgcca ccggcgccct
     1801 gctgcggacg ctcaccgggc acacggggcc ggtgtggggg cttgccatca gcgcggacgg
     1861 cctgaaactc gcctccagtg gcagcagcga catgactatc cgcacgtggc ggctcgcagc
     1921 gccgcagcag accgagacat tcgtttccgg caccatcacc acgcaaacgt ggtacaaggc
     1981 gaattcgccc taccgtgtga cgggcaccat caccgtcccc gccggcaaca cgctcaccat
     2041 tgaaccgggg gtggacgtgc tgttcaacgc ggatgtgcag ttcatcgtgc agggaagcct
     2101 gcacgcagtc ggcacgccgc aggacagcat acggttcgtg aagggcacgg caacggagtg
     2161 gggcgggctg cgcatcagcg ggggggattc cagcacaatc gcgtacgcac gtgtcagcaa
     2221 tgggcgagcg gacggcaacg tgccggacgt attcggtggc gggatctatg ttgccggtgc
     2281 cggcacgcgg ctgggcatgc ggtcagtggt ggtgagcggg aacatctcga accagcgcgg
     2341 tggcggggtg gcggtgcgcg atggagctca ggtgacgatg gatcgttgcc tgatctcgaa
     2401 aaactgggcc attgactacg gcggcggcgg actggatgtg gagaacggcg ccgtgctcac
     2461 gatgaaccgc agcacggtga cgggaaacac cgggacaccg ggtacggcga ttctgctcaa
     2521 cacggcgacg tggaacgtca cgaattcgat catctggggg gtgtatttcc cactcgctac
     2581 gccgactttc agcgcgtcgt acagcgattt ccagtcggtg tacgaaggca ccggcaacat
     2641 caatgcggac ccgctcttcg cggatgccgc caatggcgat ttccggctcc ggccggggtc
     2701 gccctgtatt gacgctggcg atccgacgtc gccgccggac gccgacggca cggttgccga
     2761 catgggggcg ctgccgcgga tgttccacat cggggacgtg acgcgggacg atgccttcac
     2821 ggctctggat gcctcgtggg tgctgcagta cagcgtgaaa ctgcgggagt cgctcccgct
     2881 ttcgctggct gacgtatcgg gcaacggccg ggcctcggcg tacgacgcgg ggctgatcct
     2941 gcacaaggcg atcatcgatc tgtcatttgt cttcccggcg ggcgatattg ccggagggga
     3001 gaaggtcggc cgtggaacgc cgcgctcgat ggggtttgtg cgggctgacg acgggtggaa
     3061 cctcgtcgtc agcaatccgg acggggttct cgggtgcgac ctgacactac tgctgccgga
     3121 gaccgcggaa gcaacgttca gcggagaggg cgcggtcgag catgcgcggg acggcggtac
     3181 tgtgctggtc ggcatcgcgc ggacggagtt cacgaatccg gtgctgctac atgtatcggg
     3241 aaccaccgac ccgccgcaga ttgtccgcgc gtcgctgaac gaaggggcga tccctgccgt
     3301 agtgaacgcg ccgctggcgt tctcgctctc ccagaacacc ccgaacccgt tcaacccgtc
     3361 cacgacgata cgcttcagcc ttcccgaggc gggacccgtg actctggcgg tgtacgacgt
     3421 aaacggcaga cttgtgcgca atctggtggg cgtcgccgcc tcggcggcgc agacattcct
     3481 gtctggccag cattccgtcg tctgggacgg ccgcgatgac aacggacgcg aggttgccag
     3541 cggggtgtat ctggcgcgcc tgacggccgc acacggtgtg ctgacgagga ggatggtgct
     3601 ggtgcggtaa cggtgtttgc aggtaggggc ggaccggcgt gtccgcccgc gttggcacac
     3661 atctcgaaca cgggcggaca cacaggtccg cccctacgat ggcatggatg cggcgcatcc
     3721 tgtgcgttcc ggatgacccg gaaggacctg tgaggcaggc ggcaggcagg ggtgcggtgc
     3781 ttgccgcccg cgcagacgcc gtggccgggt caccaggcct ccacgcgcga tccgctcagc
     3841 ccttccccgc cccgcgggca atttcaccaa tccgatagag gaggtagatg aaccagaccg
     3901 cgccgagcga gagcagggtg atggcgccga ttttcaggtg ggggactccg aagagcatcg
     3961 ggtagtagcg gtaggcaagg tagatgggta ccgcgacaat ggtccagcca atccagagca
     4021 aggctgccgt ccaccgcaga cggctaaggc ggcgggcaac ttcgggcggg tagtcctcca
     4081 tgatcacccc tttccggcca gcccgcgcag ggcggatgcc atggcgtcgg cagttacacc
     4141 gaaatgggat gccgtccaca cgaccttccc gtccgctacc agaagcgcct gcggagaggc
     4201 gtgcggggtc tgtaaccgtt ccgcaagcca gagagaaacc ggccggtttt ccaccacaag
     4261 cacgcgcgcg agggtgacac cggcggcttc aggcgtgcgt gaaaacgcct ccacctcgcc
     4321 tgccgcccgc ccgcttaccg ggcaccgcgt gctgtgcttg aacactatca acgggcgctc
     4381 ctttgccccc gatatcaacg cttctgcctg ctcgaccgag gtgatgtcga ccatctgtgg
     4441 ttctccttgt gtgccaacgg ggtagtacct aaatgtcgca aaaaaggaac gccgaggaga
     4501 cgtggtcagg tcgctcgcga cgggatggcg cacagacgcc gcgtccgccc tgcgggcgga
     4561 cggtattcct tgaccgtatc cgcccgtttt gcttaacttt atgttacaga ggatttccca
     4621 ctgaacttcc cgtattacct gttttcaggt ctatcaggag cggcacatga cgcgttccct
     4681 tcccgttttt ctttcccgcg ccggcgaccg cgtttcgctg tgtcttaccg ccattgcgct
     4741 tgcggggatg ttcctgctcg ccggttgccg ggtaaccgac cgcccggcag tgaccgtgac
     4801 gacggtggac gcgcagaacg gcgtgcgcat cgccagcatg cccgcggtga tggtgggcgg
     4861 gttcggcgtg ggcgcggggc aggtgcagag cgtcggcccg gtggcggtga ccgaggacgg
     4921 acagctttac attgcggata acacgcaacg gcgtattacc gtcatttctg ccgaaacagg
     4981 ccctcttccg acacgctcct atcttccggg gctcgcaaat ccgctcgtcc ccggcgacac
     5041 ggtgcatgtc gtccgcgcca tggaatggca cgggcccaca ctctacctcg tcgatcgcgc
     5101 acgcatctgg gccattcccc gttccgcagg gcccggcgca caggccagcg cgatagaacc
     5161 ggaaggcgga gtggccgatg tgagagatat tgaagtggcc gcggacgggt ccctgtacct
     5221 cctttcggag aggggcatcc gtatcctctc cccgtcgggc ggccttcgcg acagcatcct
     5281 cgttcgtggt gaaacgccgc cccgttgcgt cgcgttttcc ttcgggcctg acagcaatct
     5341 ttatatcagc acgcgatcgc tgaaccacgt ggtcgtctac aatcgcagtg gccaactggt
     5401 gaagcgcatc ggcgccggca cgtttgcggg cgaagcgcgc gggatcgcgg ttgaccagtt
     5461 cgggaacgtg tttgtccgcg acgagaagca agcggcgatc cgggccttta gcgcggatgg
     5521 cacgtatcgg gggaagatag gcgcgcgggg gtccggcccc ggccagatgc tctctgcaga
     5581 gcagcttgtc attgaccgtg ggcggcgcgc gctggttgtt cccgacccga ccaattaccg
     5641 cgtgcaggtt tttgatctga caggccgcag agccgaggct cgcgatcttg ttacccatac
     5701 ccccgcgttc gaacgcacgc gccgcccgta ccacatcact ctcgggatgg gcgtggacgc
     5761 gtcaacgcag cttcagatcg cctggaaaac tgcccccgct tccaccggca gtatggtcgt
     5821 ctacgttccc gtggacggtg gagacatctc tgccgttgac tggagcgaac cgggggtcaa
     5881 gaccgcgcga ggcagcagta ccgcgttcgt ctcgaatatc ggcccgtaca gagcgcactc
     5941 cgtcgccctc accgcgcttg aaccgggaac tcgctacgcc tatcgtgttg gtgacggatc
     6001 gagcgaggga tggagcgaaa cgaatacgtt cctcaccccg cccgcgtctc cggaaagcct
     6061 tgtggttgcc atactcgggg attcgcgcaa ccgcatggat gtgtggggca acatcgtgaa
     6121 ccgcgccgcg gcgtttgatc cggccttcat cgtcacaaca ggggatctgg tcagcaacgg
     6181 cgaggatttc acgcactggg atccctggtt tcatgaagcg gacgctgtgt tcaagaagac
     6241 tccgttcatg acgacccccg gcaaccacga acgggaatcc cccaactact acctttcgtt
     6301 cgaaatgccg agaaatgcgc ctgcccacct ccaggagaaa tgcttcagtt ttgacgtagg
     6361 ccagacgcac tggatttctc tcaacacaca ggtggacctt gcgcctcaga ccgcgtggct
     6421 ggaagaggac ctgaggaaaa acacgaaacc gtgggtgttt gtgtttctcc atcgcccggc
     6481 gtacgccgcg cacgcctcac ggggagacgg gaaccgggat gtgcgtgagg cgtggtgtgg
     6541 cctgtttgaa caatacggcg ttgatgtggt ctggcagggg cacgatcact actaccaccg
     6601 gtcaaagcct gttcgcgctg ccaacgtcgt tccggaaggc cgtgggccga tctacatcac
     6661 caccggcggc gccggcgctc cgttgtacca gatgaagcca aatcagtata cggaagtggg
     6721 cgaaatcacg gaccatttct gcatgatgac gctgcgcggg aatcactgtg aagtcgttgt
     6781 gtatagagcg gatggatccg tggcagatcg cttctccgtc agagactgtc cgcgtgaaac
     6841 tgcgcgatga ggtagccgtg gaacacacca tcgcaggctg ggcgctcatt ccgacgcgcc
     6901 aggaaacgtt tgatccggca tggcgcgaag tgcgccccgc aggagccgtg tgggagagcc
     6961 agcgcgtgcc ggttcctcat ggaaacgaag ccattctctc gtggaatgtg ccaccggagg
     7021 tgcagcccgg gctcgcgttc gaaatgtccg ggcaggtgcg cggcaggtgg tcaggttgga
     7081 cgcgcgtggg ggagtggggc gaagccccgg cgcccggcgt cgacgaccct ctcctccggc
     7141 gcgaggtgga caccgtagtg tgggacgccc acgtggacga gcttcgcgtg cgggttcgaa
     7201 ccgcgacggg cgaacacctg aaacttgatc ggctcgttct tgcatttccg gcggcggccc
     7261 cgaaagagag catagctctt cccaccacgt tcaatcagga acttccgttc cgctcccaga
     7321 gaaccgagga cgagagcatc ggtgatcgca tttgcggtgc aacgtgcgtt gcaatggcgc
     7381 ttgcgccccg ggttcccaat gtcacggcgg aagcaatcgc gaaggctgca tacgacccgg
     7441 tgcacgatat ctatggcaac tgggcgcgtc tggcggcagc cgccagccag cacggcgcgg
     7501 tcgcatgggt gcaacgtctg gcatcgctcg atgacgtgtt cgcacgactc gtggcgggat
     7561 accacatcat ctgctcggtg gcgtttgaaa acgggaaact cacgggcgcg ccggtgcaga
     7621 acacttccgg gcacctggta ttgatccggg gttggaacaa aaagggcgac gtggtgtgca
     7681 acgacccggc gttcactgac tcgcgcggcg atggtgtggt gtaccgccgc agcgagttcg
     7741 agcgcctctg gcttacgcac ggcggggccg cgatcatttt gcgctgggaa taggcggttc
     7801 agaacgagta cgccgcctga agcccgcgga cggggtggtt tcccgcgacc aggagcgggg
     7861 caacctgtac acgtgagacc gctgcatcca cgcgatcgta gttgcgggtg agataccacc
     7921 cgccgcccag cccgagcgtc gcggcgacca tcacgatact gactcccacc ttcgggctgt
     7981 ccacgtttgt taatgcaaca gcggctcccc ctaccaatcc tcccacgatg ccgccaaggt
     8041 tggccagccg aactctcccc tgcgacgcct cgatcctgtc ggccagaagc actcccgtcg
     8101 cgacaccaat atcgccggcc gcaagcgtgg ttccgagcac gaagcggcca ttttccgtac
     8161 ccgtgaggac catcacccag agccagtacg cgcttgacca cgcaggcgca gtggtgatga
     8221 aggtcgcatc ccccgaggat attctgtgct gaccggtgag tgcggcggtt gccagtattc
     8281 ccgcgccacc tgtaaggagc cctgcggtga tggcggcatc tgccgagggt tcatccacaa
     8341 gggcgttcgt ccagccaatt ccctgccaca ttccccagct gcccgcaaac gatatgagtg
     8401 aggcgcgggc gtcgcttacc gggcgagtct tgctccactc cagcggcgcg tacaacccgg
     8461 cggcgccacc cgccagcatg gacaatgtga acccgtccac gccgaaatcg gcaatggagg
     8521 ccagaa
//
LOCUS       DAOSOO010000141         8303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8551_length_8303_cov_6.391246, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000141 DAOSOO010000000
VERSION     DAOSOO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8551_length_8303_cov_6.391246"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT86_10065"
     CDS             <1..283
                     /locus_tag="PLT86_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT36997.1"
                     /translation="ARGGNANGVVEVAEYRAWEKKRTRDLSEISTARKAVMLTGLTSA
                     LGLYVWNIFDAHSQAEDYNRQLYRDLTGVRVGLCPGPHGMRGMMSVPIR"
     gene            complement(338..>2710)
                     /locus_tag="PLT86_10070"
     CDS             complement(338..>2710)
                     /locus_tag="PLT86_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOT36998.1"
                     /translation="TALRPAVWAVAFWIAGELCGFEWGGSPGLLCAGAFLFAVLAGVA
                     LVRQKRGIVALAALSGACVLLGWGRVALWQETRPSPAILSRFFDQRVVLSGTLTSTPE
                     PRGRVWRFGLKADTLASAENAVLGPVDVFVRMDTSLGAPCIGQRVRIAGSVRALPRRS
                     APGTMDYGEYLRYRGYSATLAAYEPGGFAPLPVPRNAWTAFWDHARSYVKRAITVGMP
                     DEAAGLASGLVLGERHGLSRRTMEAFSACGVIHILSVSGFHVAIVLVGIGVPLRRFFG
                     FGLWQALVLIACAWCYSQLAYNDPPMVRSCIMATVLLLAYTVERRSDPWNALALSALI
                     TLLVQPTALHDIGFQLSYAAMAGLMGFGSMAEERLKRFRFLQQHAWVRYPISLAVATV
                     AANITTAPLTAHYFWRMSLLALVGNLPITALVTVGVWASVAAALVGWIPFAAIAINAA
                     NARVLEAVLRLGDWFAEWPYAATWVRSPTWPEMLVYGVGTCAIWWISQRKGLRKGALF
                     ACLIYANIAVWWIWIHRPGTTEIAFLDVGQGDAAVCRFSTGHTIVIDGGPATAGYDAG
                     DWVVVPYLRSRGIRRVDAIVVTHSDSDHIGGLPSVAQAFDTRAVWYNGSGDTASAFRA
                     LESNIRQRGGRLQVAGAGDSVAGLGTAQMTFLSPMRDSAWSSRCSDNDRSLVLRIVEY
                     GDTVLFTGDTGEKAERAYSRLLPTESGARVDVLKVAHHGSRFSTGETLLATARPRYAV
                     ISVGRNMYGHPARETLEQLRATGTTVFRTDRQGTLIWRTGPAGGRWVEWR"
     assembly_gap    2711..2810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2875..5049)
                     /locus_tag="PLT86_10075"
     CDS             complement(2875..5049)
                     /locus_tag="PLT86_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT36999.1"
                     /translation="MNERIARIRTRALRSYGVHDPERDALRKESFAQTEGEPTVIREA
                     KAWAHYRRNQTLVILEDELIVGGHIGIAYDPEKNSVPEIFGRQGFKCFWNVSDEVQKY
                     FNEGMLSGAGNHTTMDYATIFEIGFEGLIQRIRNRAARLQQDEHDLLEKRDFLAALEI
                     MAEGYIALCDRYADYAAELAAETIDETRRAELETISRNCRRVPAKPPTTFWEACQAAW
                     FCFFFLADAPGRVDQYLYPFYRSDLEKGLITPDFARELLSCLWVKYFELAGARGAVSA
                     FHHLTLGGVHADGSDASNDVTMLCLDVTEDLRLNRPQIGVRWNRNMPREVLRRAVRVL
                     RTHCGNPDFCNDEQIVPALVRTGIRIEDARDFSLSGCHEVIVTGKAQMGSVEGFINLP
                     KVLRLMLGLEPNLGPGANLAAITDEETLWAAFEEAMDAVAERAHQASLARDIAAAKEP
                     GGNLHASLVVNDCIENVRGYTQGGARYNHCNWDVVGIANVTDSIATIRSLVFENGELT
                     LQELADIVRDNWQGHEALRQRVLNRCPHFGNDDEAVDRIAVRIIRTMSDIMKRRKPFR
                     GGEYILGTLAGGENMHVEFGRMTGATPDGRLNGNTFADSIGPVHGRDRRGVTAMLNSV
                     AKLPHALLPTAVSLNIKLNPELIADERGVNNVAALIEGHFLAGGQQCQFNMVTREMLE
                     DARENPEKYRGLMVRVAGYSAPFTSLWADLQEEIIARTEHTC"
     gene            complement(5311..5808)
                     /locus_tag="PLT86_10080"
     CDS             complement(5311..5808)
                     /locus_tag="PLT86_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37000.1"
                     /translation="MTCTDFRLLAERRAAGFPLKARQERALERHLAECSACAAVARFE
                     NGLAAAVREFPAVAPPADFADMVLAQLPDPVTVPEPSFREYWWNLALGVLAAGAASWA
                     LWEKAPSWIPWLTGERERTEIADILFKTYMSMGEVSLPTMSVVFLALIGLTAWGASAL
                     VAGER"
     gene            complement(5805..6398)
                     /locus_tag="PLT86_10085"
     CDS             complement(5805..6398)
                     /locus_tag="PLT86_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT37001.1"
                     /translation="MAAGDSELVRQTLKGDKRAFGVLIQRHQDAVFALVSRLIRNPGD
                     VQDVAQDAFIRAYKALPTFRGDAQFGTWLYRIVWNTCLDRREQHARVQEREVQIAADE
                     EDSDGIDGFADEETPLPDAAFEADDVRQRLEACLNRLPVHYRAVLTLYYFEQKSYEEI
                     AMVLDQPMNTIKVHLFRAKSHLKKALLAERSSEEWTE"
     gene            6612..8045
                     /gene="proS"
                     /locus_tag="PLT86_10090"
     CDS             6612..8045
                     /gene="proS"
                     /locus_tag="PLT86_10090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT37002.1"
                     /translation="MPGLTNPSSDYSAWYNEVVLQGELADYAPVRGCMIIRPYGYAIW
                     ELMQQALDGMFKETGHQNVYFPLLIPESFLQKEAEHVEGFAPECAVVTHGGGKKLEEP
                     LVIRPTSETIIWNTYRDWIQSYRDLPLLYNQWANVLRWELRTRLFLRTLEFLWQEGHT
                     AHATQEEAVEETHRMLGVYRRFAEEWMALPVIPGIKTESEKFAGAVQTLCIEAMMGDR
                     RALQAGTSHFLGQNFAKAFDVTYQDETGGRQHVWATSWGVSTRLIGALIMGHGDEKGL
                     RLPPRLAPYQVVVVPIYKTDEEKTAVLESAHRLVDFLKTSARVKLDDRDTVRPGFKFN
                     EWELRGVPLRVEMGPKDLAKGQVVLARRDTGTKEFLPQEGLAEHVRATMDDIHRTLFE
                     QAKAFRDANTFRVDTWEEFRERTAGEGGSGFLLAHWDGAAETEALIKEETKATIRCIP
                     LDNPQEAGKCVRTGKPSTQRVVFAKAY"
     gene            8054..>8303
                     /locus_tag="PLT86_10095"
     CDS             8054..>8303
                     /locus_tag="PLT86_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37003.1"
                     /translation="MTKTQHYRETLARLIDWEPFLLAESGLPGPRGNLELAAVVADMG
                     SRAQFERFLSVPDERAQVNSPGEFLVFCGVVGLGRLLAE"
BASE COUNT         1564 a         2670 c         2525 g         1444 t
ORIGIN      
        1 ggcccgcggg ggcaacgcca acggcgtagt tgaggttgcg gaataccgcg cgtgggaaaa
       61 gaaaaggacg cgagacctga gcgagatatc caccgcgcga aaggccgtga tgctgaccgg
      121 gctcacgagc gcgctcggtc tctacgtctg gaacatcttc gacgctcact ctcaggcgga
      181 ggattacaac cgtcagcttt atcgagacct caccggcgta cgagtcgggc tctgccccgg
      241 gccgcacggt atgcgaggga tgatgtcggt tccgattcgt tagcagtggg tggttgaacc
      301 ggggcgcagg ggcattggtt tggtctctgc gcccctttca tctccattca acccacctcc
      361 ctcctgccgg tcccgttctc cagatgagcg tgccctgccg atccgttcga aacacggtgg
      421 tgccggtcgc gcggagctgc tccaacgtct cccgtgcggg gtgaccgtac atgtttcgcc
      481 cgacgctgat aacggcgtac cgcggccggg cggttgccag cagggtctct ccggtggaga
      541 agcgggatcc gtgatgagcc accttgagga catccacgcg cgcacccgac tccgtaggaa
      601 ggagtcggct gtacgcccgc tccgctttct cgcctgtgtc gccggtgaaa agcaccgtat
      661 cgccgtactc cacgatccgc agcacgaggg agcgatcgtt gtcggaacag cgcgaggacc
      721 aggctgaatc gcgcatcggg gacaggaatg tcatctgcgc ggtacccagc cccgcgaccg
      781 aatcacctgc ccctgcaacc tgaagccgcc ccccacgctg ccggatgttc gattccagcg
      841 ctcgaaacgc cgacgcggtg tcgcccgacc cgttgtacca gaccgcacgt gtatcgaatg
      901 cttgcgcaac tgagggcagg ccaccgatat gatcgctgtc gctgtgcgta accacgatgg
      961 catccacccg gcgaatgccg cgcgaccgaa gatacggcac aaccacccag tcaccggcgt
     1021 cgtagccggc tgttgcgggt cctccgtcaa tcacgattgt gtgcccggtg gaaaatcggc
     1081 acaccgcggc gtcgccctgt cccacgtcga ggaacgcgat ctccgtcgtc ccgggccggt
     1141 ggatccatat ccaccacacg gcaatgttcg cgtagatgag gcaggcgaac agcgcgcctt
     1201 tccgcagacc ctttctctgt gaaatccacc agatcgcgca ggtgcccacg ccgtagacaa
     1261 gcatctccgg ccatgtgggg gaacgaaccc aggtggccgc gtagggccac tcagcgaacc
     1321 aatctccgag ccgcagcact gcctcgagga cacgtgcatt tgcggcattg atcgcaatcg
     1381 cagcgaatgg aatccatccc acgagcgccg ctgccacgct tgcccacacg cccaccgtta
     1441 caagcgccgt gatcggaagg tttccgacaa gcgcgagcag cgacatacgc caaaagtaat
     1501 gtgcggtgag cggcgcagtg gtgatgttgg ccgcaactgt cgcgacggcg aggctgatgg
     1561 gataccgcac ccacgcatgc tgctggagga accggaaccg tttgagtcgc tcttccgcca
     1621 tcgagccgaa gcccatcagg cccgccatgg ccgcgtagga gagctggaac ccgatgtcgt
     1681 gaagcgccgt gggctggacg agcagtgtga tgagcgcgct gagggcaagc gcattccacg
     1741 ggtccgagcg acgttccacc gtgtaggcaa gcagaagcac cgtcgccatg atgcacgagc
     1801 ggaccatggg cgggtcgttg tacgcaagct gcgagtagca ccatgcgcac gcgatcagca
     1861 ccaacgcctg ccagagcccg aacccgaaaa accggcggag cggcaccccg atccccacca
     1921 gcacgatggc gacgtggaat ccgctcacgg aaaggatgtg gatcactccg cacgcgctga
     1981 atgcctccat cgtccggcgc gagagcccgt ggcgttctcc cagcaccagt cccgacgcga
     2041 gcccggcggc ctcgtccggc attccgactg taatcgcacg cttgacgtaa ctccgcgcgt
     2101 ggtcccagaa tgcggtccac gcgtttcgtg gcaccggcag cggagcaaat ccgcccggtt
     2161 cgtacgcggc aagcgtcgct gagtatccgc gatagcggag gtattcgcca taatccatgg
     2221 ttccgggcgc ggaacgacga ggcagagctc gcactgagcc ggcgatccgc acgcgctggc
     2281 caatgcaggg agcgccaagg gaggtatcca tccgcacgaa cacgtcaacg ggccccagca
     2341 ccgcgttctc cgccgaagcg agcgtgtccg ccttcaggcc gaaccgccac acgcgcccac
     2401 gcggctctgg agtgctggtg agcgtcccgg agagaacgac gcgctggtcg aaaaagcggg
     2461 aaagaatggc cggagatggg cgggtttcct gccagagcgc gacgcggccc cacccgagga
     2521 gaacgcacgc gccgctcagc gcagccaggg caacgatgcc tcgtttctgg cgaacaaggg
     2581 ccacgccagc cagcacggcg aacaaaaacg ccccggcaca caggaggccc ggcgaaccgc
     2641 cccactcaaa tccgcagagt tctcccgcaa tccagaacgc taccgcccag acggcgggcc
     2701 ggagagcagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcgccgcag
     2821 ccgctccggc gacctgcccg gtcgccgccc gttgtatcga gacgaccgcc ctcctcagca
     2881 ggtgtgttcc gtccgggcga tgatctcctc ctgcagatcc gcccagagcg aggtgaaggg
     2941 cgcgctgtat cccgcaacgc gcaccatcaa cccgcggtat ttctcgggat tctcccgcgc
     3001 gtcctcaagc atttcgcgtg ttaccatgtt gaactggcac tgctgtccgc cggcgagaaa
     3061 gtgcccttcg atgagcgccg ccacgttgtt cacgccgcgt tcgtccgcga tcagctccgg
     3121 attcagcttg atgttgagcg atacggcagt gggcaggagc gcatgcggca acttcgccac
     3181 cgaattcagc atcgccgtta ccccgcggcg gtccctgccg tgaaccgggc cgatgctgtc
     3241 ggcgaacgta ttgccgttca ggcggccgtc aggggtcgca cctgtcatac ggccgaattc
     3301 cacgtgcatg ttctcgccgc ctgccagagt gcccaggatg tactcgccgc cacggaacgg
     3361 tttccgtcgc ttcatgatgt ccgacatcgt ccttatgatg cgcaccgcga tgcgatccac
     3421 cgcctcgtcg tcgttgccga aatgggggca gcggttcagc acgcgctgcc ggagcgcttc
     3481 gtgcccctgc cagttatccc ggacgatgtc ggcaagctct tggagggtga gttcgccgtt
     3541 ttcaaacacc agcgagcgga ttgtcgcgat ggagtcggtc acgtttgcga taccgaccac
     3601 atcccagttg cagtggttat accgtgcgcc gccctgagtg tacccacgga cgttttcgat
     3661 gcaatcgttc acgaccagag acgcgtgcag attcccgccc ggttctttgg cggcagcgat
     3721 gtcgcgcgca agagacgcct ggtgcgctcg ctctgccacg gcatccattg cttcctcgaa
     3781 ggccgcccag agcgtttcct catccgtgat ggcggccaga ttggcgccgg ggccgaggtt
     3841 cggttcaagc cccagcatca gtctcagaac cttcggcagg ttgatgaacc cctcgacact
     3901 gcccatctgc gccttgccgg taactatcac ctcatggcag ccggaaagcg aaaaatcgcg
     3961 cgcgtcctcg attcggattc ccgtcctgac aagcgccggc acgatctgtt cgtcgttgca
     4021 gaaatccgga ttcccgcaat gggtgcgcaa cacacgcacc gcccggcgga gcacttcccg
     4081 gggcatgttt ctgttccacc gtacgcctat ctgcgggcgg ttgagcctca ggtcctcagt
     4141 gacgtcgagg cacagcatcg tcacatcgtt gcttgcgtca ctgccatcgg cgtgcactcc
     4201 gccgagcgtg aggtggtgaa acgcgctcac cgcgccccgt gcccccgcaa gttcgaagta
     4261 cttcacccac aggcacgaaa gcagctcccg cgcgaaatcc ggcgtaatga ggcccttctc
     4321 gaggtcggaa cggtaaaacg ggtagaggta ctggtccacg cgacccggcg catcggcgag
     4381 gaagaagaaa cagaaccacg ccgcctgaca cgcttcccag aatgtcgttg gcggctttgc
     4441 gggaaccctg cggcagttgc gcgagatggt ctccagttcg gcccgccgcg tttcgtctat
     4501 cgtttcggcc gccagttctg ccgcgtagtc ggcgtaccgg tcgcacagag cgatgtatcc
     4561 ctcggccatg atttccagcg ccgccaggaa atcccgtttc tcgaggagat cgtgctcgtc
     4621 ctgctgcagg cgggcagcgc ggttccgaat ccgctgaatg agcccctcga agccgatctc
     4681 gaaaatggtg gcgtagtcca tggtggtgtg gttgcccgcg ccactcaaca tgccctcgtt
     4741 gaagtacttc tgcacttcgt ccgatacgtt ccagaagcac ttgaacccct gcctcccgaa
     4801 tatctccggg acgctattct tctccgggtc atacgcgatg ccgatgtgcc ccccgacgat
     4861 cagttcatcc tcgagtatta cgagcgtctg gttccgccgg tagtgggccc acgcctttgc
     4921 ctcgcggatg accgtcggct ccccttcggt ctgcgcgaag gactccttcc ggagcgcatc
     4981 gcgttcgggg tcgtgcacgc cgtagctgcg aagggcccgg gtgcggattc gggcaatccg
     5041 ttcgttcatc gagcatctcc tgaggggttc gcgtggcgag ttatcccacg ccgtcaaccg
     5101 gacatcaacc tctttcccgg ggaatagaag tactcacctg agggagtcgt ggagcaacgg
     5161 gtggatctgc aatgccaagc gcggcacggg ctggccgggg gcaacgtagg ggcggacctg
     5221 tgtgtccgcc cgcgctggca cacaattcga acacgggcgg acactatcct ccttgtcacc
     5281 acgccttgcg ccgatgtcga atagcagcgc ctacctctct cccgccacaa gcgcggaggc
     5341 gccccacgcg gtgaggccga tcagggccag gaaaacgacc gacatagtgg gcaggctgac
     5401 ctcccccatg gacatgtagg ttttgaacag gatgtcagcg atctccgttc tctctctttc
     5461 gccggtaagc cagggaatcc agctcggcgc cttctcccag agcgcccacg aggcagctcc
     5521 tgccgccagc actcccagcg ccaggttcca ccagtattcc cggaatgagg gttccggcac
     5581 ggtcaccggg tcgggaagct gcgccagcac catatcggcg aaatcggccg gcggagcgac
     5641 ggccgggaac tcccgcaccg cagccgcaag gccgttttca aaccgcgcaa cggcggcgca
     5701 ggcggagcat tccgcaagat gccgctcgag ggctctttcc tgtcgggcct tcaaagggaa
     5761 accagcggca cgccgttcgg caaggaggcg gaaatcggtg catgtcattc ggtccactcc
     5821 tcggaagaac gctcggcaag gagcgccttc ttgagatgcg atttcgcgcg aaacagatga
     5881 accttgatcg tattcatcgg ctgatcaaga accatcgcaa tttcctcgta acttttttgc
     5941 tcaaagtaat acagcgtcag aaccgcgcgg taatggaccg gcagccggtt caggcacgcc
     6001 tcaagccgtt ggcgaacgtc atccgcttcg aacgccgcat ccggcagggg tgtttcctcg
     6061 tcagcaaacc cgtcgatacc gtcggaatct tcttcgtccg cagcaatctg cacctcgcgc
     6121 tcctgcaccc gtgcgtgctg ttcgcgccgg tcgagacagg tgttccacac gatgcggtag
     6181 agccatgtcc cgaactgggc gtcgccccga aacgtgggga gagccttgta cgcgcggatg
     6241 aaggcgtcct gcgccacgtc ctgcacgtca ccggggttgc ggatgagccg gctgacgagg
     6301 gcgaacaccg catcctggtg gcgctggatc aacacaccga aggcgcgttt atcgcctttc
     6361 agtgtctgcc gcaccagttc gctgtcgcct gccgccaaaa ccgcgcccct tgaatcacgt
     6421 acccactgaa cgatatgacg gtcttccgtg tggaaaagtt acagtggaag caggaaggcg
     6481 caaggccggc acgggaagcg cggcgccggc cggctggccg agatgccccg ttcgtgcgta
     6541 tctttctgta ttcccattcg ataacaaaca cggtcgcttg cagaaacccc atccatctca
     6601 ggaagaactt catgcccgga ttgacaaacc cctcctcgga ttactccgcg tggtacaacg
     6661 aggtcgttct gcagggcgag ctcgctgatt atgcgcccgt gcgtggctgc atgatcatcc
     6721 gcccctacgg atacgccatc tgggagctga tgcagcaggc tctcgacggc atgttcaaag
     6781 aaaccggcca ccagaacgtg tatttcccgc tcctcatccc cgagagcttc cttcagaagg
     6841 aagccgaaca cgtggaaggc ttcgcgccgg agtgcgccgt ggtaacccat ggcggcggca
     6901 agaagcttga ggagccgctc gtcatccggc cgacctccga aacaatcatc tggaacacgt
     6961 accgggactg gatacagtcg tatcgcgacc ttccgctcct ctacaaccag tgggccaacg
     7021 ttctccgttg ggaacttcgc acgcgcctgt tcctccgcac gctcgagttc ctctggcagg
     7081 aaggccacac ggcgcacgcc acgcaggaag aagccgtgga ggaaacgcac cgcatgttgg
     7141 gcgtgtaccg gcgctttgcc gaggagtgga tggcgctgcc ggtgattccg ggaatcaaga
     7201 ccgaatccga gaaattcgcc ggcgcggtgc agacgctctg catcgaagcg atgatgggtg
     7261 accgccgcgc gttgcaggcc ggaacctcac actttctcgg ccagaacttc gcgaaagcgt
     7321 tcgacgtgac gtatcaggac gaaacgggcg gtcgccagca cgtctgggcg acaagctggg
     7381 gcgtcagcac gcggctcata ggcgcgctca tcatgggaca cggggatgag aaagggctgc
     7441 gcctgcctcc gcgtcttgca ccgtatcagg tggtcgtggt gccgatctac aagaccgacg
     7501 aggagaagac ggcagttctc gaatcggcgc accgccttgt ggatttcctg aaaacgagcg
     7561 cacgcgtgaa actggacgac cgcgataccg tccgccccgg gttcaagttc aatgagtggg
     7621 agctgcgcgg cgtgccgctt cgggtcgaaa tgggccccaa agacctcgcg aagggccagg
     7681 tggtgctggc tcgccgcgat accggcacca aggagttcct gcctcaggaa gggctggcag
     7741 agcacgtccg cgcgacaatg gacgacatcc atcgcacgct gtttgagcag gcaaaggcct
     7801 tccgcgatgc caacacgttc cgcgtggata cgtgggagga atttcgcgaa cgcaccgccg
     7861 gcgaaggagg cagcgggttc cttcttgcgc actgggacgg cgcggcggaa accgaagcgc
     7921 tgatcaagga agagacgaag gcgacaatcc gctgcatccc gctcgacaac ccgcaggagg
     7981 cggggaaatg cgtccgcacg ggcaaaccct cgacacagcg ggtggtcttc gccaaggcgt
     8041 attgattgaa cggatgacga agacgcagca ctaccgcgaa acgctggcac gccttatcga
     8101 ctgggagccc tttctcctcg cggaatccgg cctcccgggc ccgcggggca atctcgagct
     8161 ggctgcggta gtggcggaca tgggtagccg cgcccaattc gagcgtttcc tttcagttcc
     8221 ggacgaacgc gcgcaggtga attctcccgg cgagttcctt gtcttctgtg gtgttgtcgg
     8281 tctggggcgc ctgctggcgg agg
//
LOCUS       DAOSOO010000142         8284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8587_length_8284_cov_5.938996, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000142 DAOSOO010000000
VERSION     DAOSOO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8587_length_8284_cov_5.938996"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLT86_10100"
     CDS             complement(<1..217)
                     /locus_tag="PLT86_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37004.1"
                     /translation="MVFPQSLKGIAALFLIAKCGVALTPKMQTIKDISVGVGEQLPSG
                     WVGLGARSQAMGGTLAASTIDGTALFAN"
     gene            complement(252..1022)
                     /locus_tag="PLT86_10105"
     CDS             complement(252..1022)
                     /locus_tag="PLT86_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37005.1"
                     /translation="MNSTPRGKRSLAGLLILGSLSLYGCYTVLSHPQLAPRQQITENG
                     TVVETDQCASCHTQSDLWSFHHRGWYSRYNSYFYNQYDPLFFRSRWYGDSYFYGRWVS
                     YRYSPWWYYPGSRTGAWHPELPAVGRGQVRDSVNSVDPRDSFVGLPGYGPGPMPSFPR
                     SALPLNTGMAGSPATSRVTRDSTSADRGSATEGRPVRVEPRNDAPAPLPVSQQPVQQP
                     AATKSADKPQESEDQEQAGDQPRGRGDARNRPSGSQSR"
     gene            complement(1167..3530)
                     /locus_tag="PLT86_10110"
     CDS             complement(1167..3530)
                     /locus_tag="PLT86_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT37006.1"
                     /translation="MFDEHALRVIEYPLVRSLIARNAVSGMGHDLVESTTPMTDRVSL
                     ERRMQEVDEMVQAVRWGDPVPLRGVVDIRQPVELAAHPGARLDPPSLLDIAQTLEAAG
                     RLRGYFAARTQKYPRLEAISSALEPCPELVTAVNRAIDPGGFVRDSASPALAKTRREL
                     EQARSNLRANLERLVHSLASDVLTERVVAWRNGRPVIPVRAQRRNTVGGIVHDESASG
                     QTVFVEPFESVEDANRIRRLEIAEQAEVDRILSELTGVVFQHSPRLRVNLQTLGRLDA
                     LYALGVYADQADAAVPGISDDGALEIVRMRHPLLDVRLRGQKREAVPLTCRLRPDDRL
                     MLISGPNAGGKTVALKTIGLAIAMTQSGFPIPADPLTTLPVFESFFAEIGDEQSIEND
                     LSTFSSRIGHLARISAEAGKGTLILVDELGSSTDPDQGAALSRALLERWFEKGARTVA
                     TTHLGSLKEFAHEMAWAVNASMEFDRDKLNPTFRLVAGIPGSSYAFEISRRIGLAEDI
                     IARAEHFLGRAAVQTERLIADLTFLMEQTERRLREIESRERALAEREREYEDRFARVV
                     SDRKRMAREAREEAEKILADARALVERTVADIRRTNGDRESIKSARATLQSALARTRS
                     ELADRGRTDGAEATGINPGNWVKIVSINKTGEVVAVSKDRVAVQTGGTRLEVRPDQVE
                     KIAEKAPDAGKGGVSVTTTASDRFLPEVDVRGMSFDDAWAILDRYLDDACVARYPRVR
                     IIHGKGTGVLRRRINEQLKQDERVASHQMGEYYEGGAGVTVVDMKLD"
     gene            complement(3539..>4044)
                     /locus_tag="PLT86_10115"
     CDS             complement(3539..>4044)
                     /locus_tag="PLT86_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT37007.1"
                     /translation="RTLLWVAGFAFGLFLAARYAGGVSGLLGKTIPGSAREAIAFVVI
                     VLAVMVVVDIFNAVLEKFLRSTPLTTLNRAAGALLGILKGVLIVALVGVLLAILPLPS
                     TVAQTYARSGVVQGSTEFGGVLVDLVKDRLPEPARTFLAEARSTYVDRMRIVFPPPGN
                     TAVPRVP"
     assembly_gap    4045..4144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4145..4484)
                     /locus_tag="PLT86_10120"
     CDS             complement(<4145..4484)
                     /locus_tag="PLT86_10120"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOT37008.1"
                     /translation="MTALETRLQEDQKKAMKTGDKSLLGLIRLLRSQLQYARMQAGEE
                     WSEEKAQEVLLAAAKARRESISLFEKGGRMDLVEKEKAELALLERYLPAQLSDQELST
                     LIELCISETGA"
     gene            complement(4494..4691)
                     /gene="rpsU"
                     /locus_tag="PLT86_10125"
     CDS             complement(4494..4691)
                     /gene="rpsU"
                     /locus_tag="PLT86_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT37009.1"
                     /translation="MAGVIVREKEPFEKALKRFKKACEKAGIISDMKKHQHFEKPSER
                     RKRELNAARRKMRKTQILSEK"
     gene            5003..5365
                     /locus_tag="PLT86_10130"
     CDS             5003..5365
                     /locus_tag="PLT86_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37010.1"
                     /translation="MRKFGEIVVDEGFATEEQINSALTYQKTSDILVGKILVDMRVLT
                     HADVERVSNFLKTPECQGKRFGEAAVFLGACTEADVARAIEIQRTSKGMLGDLLVALG
                     YITERQRQDIVRIQMEEA"
     gene            5433..6467
                     /locus_tag="PLT86_10135"
     CDS             5433..6467
                     /locus_tag="PLT86_10135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37011.1"
                     /translation="MHQIHVSSLTSSRRIRVADDKGYFPVLVRAADNALMAVFRDGAA
                     HMGAGGYLVASRSTDLGLTWTEPVVVVNTREYDDRNPAVGVARNGVITVAFHANGSYG
                     PSGDYIKDRRTPRSLHTGIVQSADNGRTWGDPMLWTDATPWDGMSPYGRMLTLADGTM
                     VMSIYNGGSYLLRSRDNGQTWGGLSLTGTDTNESSYCVLPTGEWLCAGRKQRESEDQR
                     ILVRRSSDGGMTWSEPTPFGVNKTLPADLLALSNGSVLIVYGYRTPPFGVRARMSHDN
                     GRTWSETELVVHGSALSADCGYPSALVADGYVIIVFYDAGRTTHDAPDGTGAFCELVR
                     IAESELISVF"
     gene            6479..>8284
                     /locus_tag="PLT86_10140"
     CDS             6479..>8284
                     /locus_tag="PLT86_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37012.1"
                     /translation="MILRRSVLVFALLWAGYVPAETITTQSGLFRVHYAPGAKETAKQ
                     TAIIAEDVFYQLVTAYSLHERFRPIDILVTDDIDAGNGFADYYQNQLVVWATNLDTDL
                     RGTNQWLRNVVTHELAHIFSLKLARRYPFRYGLISASVLNSSVADIGVGIPIYGLVTP
                     AWWVEGIAQYEADRYGGDHWDTHRDMLLRMATLENDLLSYDEMGVFAHNWLKSEMVYN
                     QGYALTSYISRNFGANAPRNLAKNTGYVTFNTALRENLRLTGQDLYDRWVADIRARYS
                     SVRDSVGAIVEGNALPDEGSYETAPAISPDGTQIAYLSARGEDFLLTKPRIRHLARNT
                     VLDIDRSVRGNLAWFPDGGRIAYAKFGRGTYLNDLYVYDIARKEETRITSRLRARDPA
                     VSPDGKWIAFVSGEDGGTRLGIVRADGSEIRWLTNSMRSPGPGPADIGSKTSDLIQFY
                     TPNWSPDGKSLLFSVFRDGDRDIGIIRTQGPYFSVRDALSDSAAFPDTLVYPAGSGFR
                     LLVHTEADERDPAWLPDGSGFVFSADYDIATDPRNQTPEESVRSDGGSRPFGIFNLYL
                     ATLPADSSEEISYRRLTNVLGGAFRPAVAPDGRWLA"
BASE COUNT         1668 a         2431 c         2384 g         1701 t
ORIGIN      
        1 ggttggcgaa aagcgcggta ccgtcgatgg tgcttgccgc aagagtaccc cccatcgcct
       61 gcgagcgcgc acccagtcct acccatcccg aaggcaactg ctcccccacg cccaccgaga
      121 tatctttgat agtctgcatt ttcggggtga gggcaacacc gcacttcgcg atcaagaaaa
      181 gcgcggcgat gcctttcagg ctttgcggga acaccatcca gcactcctct gcctgtgtat
      241 gatgatcaac tctatcgcga ttgcgaaccg gaaggcctgt tgcgtgcgtc gccacgccct
      301 cgaggctggt cacccgcctg ctcctgatcc tcggattcct ggggcttgtc cgccgatttg
      361 gtggcagcag gctgttgaac cggttgctgg ctgaccggga gtggagccgg agcgtcgttc
      421 cgtggctcaa ctcggaccgg gcgcccttcg gttgcggaac cgcggtctgc ggaagtagaa
      481 tcacgtgtga cgcgcgaggt tgccggagaa cctgccattc ctgtgttcaa cggcagcgcc
      541 gaccgcggaa atgaaggcat cggaccgggt ccgtaccccg gcaatccaac gaaactatcg
      601 cgcggatcta cactattgac gctgtcgcgc acctgaccac gtccaacagc cgggagttcg
      661 ggatgccagg cacccgtccg gcttccggga tagtaccacc acgggctgta gcggtagctg
      721 acccagcgtc cgtagaagta agaatcacca taccaccgtg aacggaagaa caatgggtca
      781 tactggttgt agaaatacga gttgtaccgc gagtaccatc cgcggtgatg aaacgaccac
      841 aagtcactct gggtatgaca cgaagcgcac tgatccgtct ccacgacggt tccgttttcc
      901 gtaatctgtt gccgcggcgc aagttgagga tgcgagagaa cggtgtaaca gccgtacagg
      961 ctcaaggacc ccagtatgag caagccggcc agactgcgtt tgccccgcgg cgtgctgttc
     1021 actgcggaac ctccttgatc gaagatccaa tcggtacgcg ggcctctggc ggattcgcca
     1081 tatgctcatc cataagatac tctgccccga cgcgaaatgt ccgcgccgtt cgtctgaggt
     1141 tctccagtat ttggaagccc cagctgtcag tccagtttca tatccactac tgtcacaccg
     1201 gcgccccctt cgtagtattc gcccatctga tgggaggcta cccgttcgtc ctgtttcagt
     1261 tgttcgttga tcctgcgccg gagcacaccg gtgccttttc catgaatgat tcgaacgcga
     1321 gggtaccgcg cgacgcacgc gtcatccaga taccgatcca gaatcgccca cgcatcgtcg
     1381 aaactcattc cccggacatc cacttcagga aggaaacgat ccgacgctgt cgtagtaacg
     1441 gagacgccgc ccttgccggc atccggtgcc ttctccgcta tcttttccac ctgatccggt
     1501 cttacttcca gacgcgttcc gcctgtctga accgccacac gatccttgct gacagccacc
     1561 acttcgcctg tcttgttgat tgaaacgatc ttcacccagt ttccggggtt tattcccgtc
     1621 gcctcggctc cgtcagtgcg accgcgatca gcaagttcgg acctcgtccg cgcaagcgcg
     1681 ctctggagcg ttgcccgcgc gcttttgatg gactccctgt cgccattggt acgccggata
     1741 tcggcgacag tgcgttccac aagggcgcgc gcgtccgcga ggatcttttc tgcttcttcg
     1801 cgtgcttccc gcgccatgcg tttccgatct gaaaccacgc gtgcgaacct gtcctcgtac
     1861 tcccgttcac gttcagcgag cgcgcgttct ctggactcga tctcgcgcag tctgcgctcc
     1921 gtttgttcca tcaaaaaggt caggtcggcg ataagtcgtt ccgtctgcac tgccgcccga
     1981 ccaaggaagt gctccgcgcg ggcgatgatg tcttccgcca agcctatccg gcgactgatt
     2041 tcgaaggcat aactggaacc aggaatgccc gccaccaaac gaaaggtcgg gttcaatttg
     2101 tccctgtcga attccatcga cgcgttgacc gcccaggcca tttcgtgagc gaattccttg
     2161 aggcttccaa ggtgtgtcgt tgcgacggta cgagcgcctt tctcgaacca gcgttcgagc
     2221 agcgcgcggc tcagcgccgc accctggtcg gggtcggttg aggaaccaag ttcatccacc
     2281 aggatcagtg tgcccttgcc tgcctctgcg gaaatgcggg caaggtgccc gatgcgcgaa
     2341 ctgaaagtgg aaagatcgtt ctcaatcgac tgttcgtcgc cgatttccgc aaaaaaggat
     2401 tcgaagacag gaagtgtcgt gagcgggtcc gcgggaatcg gaaaaccaga ctgcgtcatc
     2461 gctatcgcaa ggccgatggt cttgagagcg accgtttttc cccccgcgtt cgggccgctt
     2521 atcagcatca accgatcgtc aggccgcagc cggcaggtga ggggtaccgc ctctcttttt
     2581 tggccgcgca gacggacgtc cagcaaaggg tgtcgcatcc ggacgatctc cagtgcgccg
     2641 tcgtcagaaa tcccgggcac ggcagcgtca gcctgatccg cgtacacacc gagcgcgtag
     2701 agtgcgtcca accgtccgag ggtctggagg tttacccgga gtcgaggcga atgctggaac
     2761 acaaccccgg tcagttctga aagaatccga tcgacctccg cctgctcggc gatttccagc
     2821 cttctgatgc gatttgcgtc ctcaaccgac tcgaacggtt ccacaaaaac cgtctggccg
     2881 gacgcagatt catcgtggac gatgccgccc accgtattgc ggcgttgcgc ccggacagga
     2941 atcaccggcc tgccattccg ccacgcgaca actctctcgg tgagcacatc actcgcgagg
     3001 ctgtggacga gtcgttccaa attggcgcgg agattcgacc gcgcttgctc cagttcacgc
     3061 cttgttttcg cgagcgcagg acttgccgag tctcgaacaa atcctcccgg gtcgatcgcc
     3121 cgattgaccg ctgtgacgag ttcaggacac ggttccagcg ccgacgaaat cgcttcgagt
     3181 cgggggtatt tctgtgttcg cgcggcgaag taaccccgca ggcggccggc cgcttcgagg
     3241 gtctgggcaa tgtcgagcag ggagggcggg tcgaggcggg cacccgggtg cgcggcaagc
     3301 tccacaggct gtcggatgtc caccacccct cgcaaaggta cgggatcacc ccatcttacg
     3361 gcttgaacca tttcgtcgac ctcttgcata cgccgctcga ggcttacgcg gtcagtcatc
     3421 ggggtggtgg attccacaag atcgtgaccc attccgctta ctgcgtttcg tgcgatgagg
     3481 gaacgaacga gtggatactc gatgacgcgg agggcgtgct cgtcaaacat cgaagagctc
     3541 agggcacgcg gggaactgcg gtgttcccgg gaggagggaa gacaattcgc atgcggtcaa
     3601 cgtaagtcga gcgtgcttcc gcgaggaaag ttctggcggg ttcgggcagg cgatccttca
     3661 caaggtctac cagaacgccg ccaaattccg tgcttccctg aacaacaccg gaacgggcat
     3721 acgtctgggc cacagtggag gggagaggga gaattgccag caacacacca acaagcgcca
     3781 cgatgagcac gcccttcaaa atgcccagca atgcccccgc cgctctgttc agggttgtga
     3841 gcggggtcga gcgcaggaac ttctcaagca ccgcattgaa tatgtcgacg acaaccatca
     3901 cggcgaggac gatcaccacg aacgcgatgg cttcgcgcgc agaacccggg attgttttcc
     3961 ccagaaggcc ggatacgccg ccagcgtacc tggccgcgag aaagagcccg aacgcgaacc
     4021 cggcaaccca caacagcgta cgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnngcgcgc cggtttcact gatgcacaac tcgatcaggg tggatagttc ctggtcggag
     4201 agctgcgcgg gaagatatcg ctccagtagc gccagttccg ccttttcctt ctcgacaagg
     4261 tccatgcggc cgcccttctc gaacagggaa atcgactccc gtctggcttt cgcggcggcc
     4321 aatagaactt cctgtgcctt ttcctccgac cattcctccc cggcctgcat ccgcgcatac
     4381 tgaagctgtg aacgaagaag gcgaatcagt ccgaggagac ttttgtcccc ggtcttcatc
     4441 gccttcttct gatcttcctg caaccgcgtt tcgagagcgg tcatttttcc gacctatttt
     4501 tcggaaagaa tctgtgtctt ccgcatcttg cgccgcgctg cgttcagttc gcgtttccgt
     4561 cgttcgctgg gcttttcaaa gtgctggtgc ttcttcatat cggaaatgat accggccttt
     4621 tcacacgcct tcttaaaacg tttcaacgct ttttcaaagg gttctttttc gcgcacgata
     4681 acacccgcca tccggtttcg cctcctttcg ctggggattt tacatggtat gctatacacc
     4741 gccggcattc gccggataga ccaattctag aatatacgac ttcccggcta cgcgtccaat
     4801 gcgatgctgg gagagagtgg gtgcaatgcg agtcttcttc tggtgacaca aaaccttgcg
     4861 gccgtgatga tctttcaggg gtggcacagc cagcgatgac gggtatcttt gtaatgattt
     4921 cgccctccgt tgtgagggcg gacaccgtaa aggacccggc acacggttgt gactcaggac
     4981 aaatggcaca ggagttgaag gaatgcggaa atttggcgaa atagtcgtcg acgaaggctt
     5041 cgcgacggag gagcagatca attctgcgct gacgtaccag aagacttcgg atattcttgt
     5101 cggaaagatt cttgttgaca tgcgtgtact cacccatgcg gacgtggaac gggtgtcgaa
     5161 tttcctgaag acacccgaat gccagggaaa acgattcggc gaagccgcgg tttttctcgg
     5221 tgcgtgcaca gaagccgacg tggcacgagc gattgagatc caacggacaa gcaaggggat
     5281 gctcggagac cttctcgtcg cactgggata catcaccgaa cggcaacgtc aggatatcgt
     5341 ccggatccag atggaagagg cgtgacacaa gctcacgcag cgctgcggcg agcataccaa
     5401 ttgccagttg cgggcgtgtg gaggcatctc aggtgcacca gatacatgtt tcttcgttga
     5461 cgagttcccg gcggattcgc gtagcggatg acaaggggta tttcccggta ctcgtccggg
     5521 ctgccgataa cgctctgatg gcggttttcc gtgacggtgc ggctcacatg ggtgcgggtg
     5581 gttatctggt ggcatcgcgc tcgaccgacc tgggtctgac atggacggaa cctgtggtgg
     5641 tcgtcaacac gcgtgagtat gatgaccgga acccggccgt cggggttgca cggaacggcg
     5701 tgatcacggt ggcgttccac gcaaacggaa gctacgggcc ctccggcgat tacatcaagg
     5761 accgccggac tccccgatct ctccacaccg gtatcgtgca atctgcggac aacggccgca
     5821 cgtggggaga tcctatgctg tggaccgacg ccacgccatg ggatggcatg tctccctacg
     5881 gtcggatgct gacgctggct gacggcacga tggtcatgtc catttacaac ggcggaagtt
     5941 atctgcttcg gtcgcgcgat aacggacaga cgtggggcgg actgtcgctg acaggcaccg
     6001 acaccaatga gagttcgtac tgcgtgcttc caacgggtga gtggctctgc gcaggacgaa
     6061 agcaacgcga gtcggaagac cagcgcattc ttgtccggcg atcctccgac ggtggcatga
     6121 cgtggtccga acccacaccg ttcggagtca acaagacgtt gccggccgac ctgctggcgc
     6181 tttcgaacgg atctgtcctc atcgtctacg ggtaccgtac tccgccgttc ggtgtgcggg
     6241 cgcgaatgtc gcacgacaac ggcagaacgt ggtcggaaac agaactcgtg gttcacggtt
     6301 ccgctctttc agcagactgc gggtacccga gtgctcttgt ggcggacggg tacgtgatca
     6361 tcgtattcta cgacgccggc aggaccacac acgatgctcc cgatgggact ggagcgttct
     6421 gtgaactcgt acggatcgcc gaatcagagc tgatttcggt gttttgagac ggaacggaat
     6481 gattctgaga agaagcgtac tcgtcttcgc actcctgtgg gcggggtacg tccctgcaga
     6541 aaccatcacc actcagtccg ggctgttccg cgtgcattac gcaccgggag ccaaagaaac
     6601 cgcgaaacag acggcaatta tcgcggaaga tgtcttctac cagctcgtca cggcatattc
     6661 cctccacgag cgattccggc ccattgatat cctcgtcacg gatgacattg acgcaggtaa
     6721 cggcttcgct gattactacc agaaccaact cgtggtctgg gcgacgaacc tggataccga
     6781 tctccgcggc acaaaccagt ggcttcggaa tgtcgtaacg cacgagctgg cccacatttt
     6841 ctccttgaaa ctggccagac gctacccatt ccgttacggc ctgatttccg caagcgtctt
     6901 gaacagcagc gttgctgaca tcggcgtggg gataccgatt tacggcctcg tcacacccgc
     6961 atggtgggtt gaaggaattg cccagtatga agcggatcgg tacggcgggg accactggga
     7021 tacccacagg gacatgctgc ttcgaatggc gacgttggag aacgacctcc tgtcctatga
     7081 cgaaatggga gtgttcgccc acaactggct caaatcggag atggtgtaca atcaggggta
     7141 cgcactgacg tcgtacatca gccgcaattt tggagcaaac gcacctcgca acctcgcaaa
     7201 aaacaccgga tatgtgacgt tcaataccgc tctgcgtgaa aatcttcgac ttaccgggca
     7261 agacctctac gaccggtggg ttgcggatat ccgggcgcgg tattcatcgg tgcgggatag
     7321 tgtcggtgcc attgtcgagg ggaatgcgct gccggacgaa ggatcatacg agacagcgcc
     7381 cgcgatttcc cccgacggca cccagatcgc ctacctttcg gcgcgagggg aagatttcct
     7441 tctgacaaaa ccccgcattc gtcatctcgc gcgcaacact gttctcgaca tcgatcgctc
     7501 tgtgcgcggg aacctcgcct ggtttcccga cggcggcagg atcgcgtacg cgaaattcgg
     7561 gaggggaacg tacctcaacg acctctacgt ctacgacata gcccggaaag aggaaacgcg
     7621 catcaccagt cggttgcgcg cacgtgatcc cgccgtttcg ccggacggga aatggattgc
     7681 atttgtgtcc ggggaagacg gcggcacacg gctcggaata gtgcgtgccg acggatcgga
     7741 aatcagatgg ttgacgaaca gcatgcgaag cccgggtccg ggacccgccg acatcggttc
     7801 caagacaagt gatctgattc agttctatac cccgaattgg tctcctgacg gcaaaagtct
     7861 tctcttcagc gtgtttcggg acggagatcg cgacataggg ataataagaa cgcagggtcc
     7921 ctatttctcg gtgagggatg ctctgtcgga ttcggccgca tttcccgata ccctcgtgta
     7981 cccagcgggg tcaggtttcc ggctgctcgt ccacacggaa gcagacgaac gagaccccgc
     8041 gtggctgccc gatgggagcg gatttgtctt ttctgctgac tacgatatcg caacagatcc
     8101 acgcaatcag acgccggaag aaagcgtgcg ttctgacggt gggtccagac ctttcggaat
     8161 cttcaacctc tacctcgcaa cgttgccggc ggattcctca gaggaaatat cgtacagacg
     8221 gttgacgaat gttctcggag gtgcgtttcg gccggcggtt gctccggacg gcagatggct
     8281 ggcg
//
LOCUS       DAOSOO010000143         8277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8600_length_8277_cov_5.226952, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000143 DAOSOO010000000
VERSION     DAOSOO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_8277_cov_5.226952"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="PLT86_10145"
     CDS             complement(<1..2104)
                     /locus_tag="PLT86_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37013.1"
                     /translation="MPHFPAERTILVRQIGNRLNELFAALYRNRTPITGWQTYVAGPG
                     QYPAPIPVDGWIPYELGDDWGGYDVTQWFKARVQIPREMAGRTVAAILNPGWEALCYL
                     NGAPAQGLDRNRSEVVLTTCAKGGEEFEIVVEASATPNQYDDNPIHQFTSAEIAERDE
                     LVWSFYWDLKVANDVVAVLPPDSQPRMRLQDLVDWAVKLIDLNNKDDVASFRANIADA
                     QKRFRAKLKDFRRCPGDGKITYVGHSHIDTAWLWPLRETRRKCARTFSSALRYMDRYP
                     QFVFSQGQPQLYEFIKENYPTIWEGIKQRVKEGRWEATGGGWVEQDSNVAGAEALVRQ
                     YLYGRRFFIKEFGVQPRLVWLPDAFGFPITLPQIIAKCQLTAFGTTKINWSQYNQFPY
                     SHFLWRGLDGTEILAFMPPGSYNSNPTPANAVNDWRSVKQKDVVDELSITFGHGDGGG
                     GVTIEMFENVLRMKDVTGLPECDFGTLEGYVERLHKSVDKEKLPVYNDELYLELHRAC
                     QTTQARTKRNNRKGELLFRDTEYLSSVAMLAGAEYPQQEIYEQWKPFLTNQFHDILPG
                     SSVNEVYTQADKDYASILGKLSSLREQALATISRGIDTAGAGTPVVIYNTLGWDRDDV
                     ASVAITGDAAVGMLDEKGKPVVSQIVTERDGQKRLLFEVSGVPSMGHSVYRMVENPRE
                     QKSRLKVSESRLENDFFVI"
     gene            complement(2156..5266)
                     /locus_tag="PLT86_10150"
     CDS             complement(2156..5266)
                     /locus_tag="PLT86_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOT37014.1"
                     /translation="MIHFPQERAIVARQIANRLGEILALQYSSIQPVEGWEYVDVGTR
                     QDVAPVPQAGWQPYELGSWWGGQDVTCWFRARAEIPEPTTGERPVCLLNPGGESLVLA
                     DGEPLQGLDANRQEVVLPRRFWGKNVELVLQSYSSPSNSNTGRRGFSVAAVALRDELI
                     RSFHWDLKVAFDASNALDNESLTSRRILEHVEKCVKAVDLNSAGDRRAFRSHIASVRK
                     IFAAGMEEFASSVGMGNVVLAGQSHIDVAWTWPLRETRRKVARTFSTVLNYMDQYPDY
                     VYFQSQPQVYEWVKSHYPSLWSRIKEQVRAGRWEPTGASWVEQDTNIPSGESHVRQFL
                     YGNRFFRREFGVHTRVFWLPDCFGFSFALPQILRKAGVEAFATTKLASNEFNEFPYSL
                     FRWRGLDGSEVLAYQMPWEWNVTPANLLAHWNRFKQKTSTDEIPMTFGIGDGGGGPTA
                     EMLENAERLKNVRGFPQARAGRFQDHFDRLMTSVNREDLPVYVDELYYEKHRGCQTTQ
                     SRTKRNNRKSELALREAEFLASCELIAGGEYPQETISEAWKTVLLNQFHDILPGSSIA
                     EVYEDAEKDYAGVRRALQNLKDHSLRSLARRIDTRGEGDALIVWNSLGWKRDDVAITP
                     LPSADSSGWSVLDPDGREVATQPVILESGAAGLLFEASDLPPMGYSVFRLVKRAPRAR
                     RNLIARRNNLENRFFEVALSKTGSISRIFDKLNNREVVPEGSSANRLVLFEDFPASAD
                     AWDIDFNFEEKFQEIGDTESVTVTERGPVRATVRVVKRTKNSRITQDISIWASLSRID
                     FATEVEWYEKHKLLKASFPVNVLSRIATYEIQFGAIERPTHRSGPVERARFEVPGHRW
                     IDLSEADYGVSLMNDCKYGFDVCENNMRISLLRGSVSPDPHADEGQHLFLYSLYPHRG
                     DWRSALTTRRAYELNAPLSCWIEKAHGGTHPRRWSFVSVDSSDIVIDTVKKAEDSDAI
                     IIRVYESHGARGEATLQFGKVPLNVTECDLMEEHDVDVPVKDSSVQLMMKPWEIKTLK
                     ATFSNG"
     gene            5678..7156
                     /gene="glpK"
                     /locus_tag="PLT86_10155"
     CDS             5678..7156
                     /gene="glpK"
                     /locus_tag="PLT86_10155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT37015.1"
                     /translation="MVLAIDQGTTSSRAIIFDVSGAILGQGYAELPQYYPQPGWVEHD
                     PEEIWSTVTEAIKQALVASNVSPAEIKAVGVANQRETTIIWDRRTGKPIHRAIVWQCR
                     RTSDICDRLRAEGWEEEIHNATGLFIDPYFSATKIRWLLDNVEGAQEAAEKGHLAFGT
                     VDSWLLWKLSGGHIHATDYTNASRTLLFDIRAKSWNQKLLSHLKIPRSLMPQVKYSSV
                     MYGKTNCPDVLPKPVTISGVAGDQQASLFGQFAYQPGQTKTTYGTGCFLLQHTGAEPK
                     VSAHGLLATLAIDETGGPAYALEGAVFNAGSAVQWLRDGLQVITSSAESEAYAEQVSD
                     TLGVYVVPAFNGLGAPYWNASVRGAMFGITRGTARHHIIRATLESIAYQTADVLDALA
                     EDSGIRIPELFVDGKATQNNFLMQFQADIAGVPVVRPVMQETTVLGAALLAGIGAGVW
                     SSAQQAQEIARLDRVFEPAMGEEQRHALRNGWRRAVAAATQF"
     gene            complement(7147..7809)
                     /gene="coaE"
                     /locus_tag="PLT86_10160"
     CDS             complement(7147..7809)
                     /gene="coaE"
                     /locus_tag="PLT86_10160"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT37016.1"
                     /translation="MRYAMTPALGLTGGTGTGKSTVAKFLAKRGARVIDADKIGHVVL
                     KTNKKVRSALEDAFGADILGENGSIDRKKLGDLVFGDPVALARLNAVVHPPLLETLRE
                     ELDRARLDSALPLVVVDAALIVEWGITSWFDALVVVTAPRGIVEARLAAKGLDLDAIA
                     RRISSQLPDEERIKNAAMVVQNDGDLVHLEEAAGRIWDSFARAVPPAQASAGGGTDAT
                     QN"
     gene            complement(7806..>8277)
                     /locus_tag="PLT86_10165"
     CDS             complement(7806..>8277)
                     /locus_tag="PLT86_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT37017.1"
                     /translation="GGRLHLIVATPTSPGVARLVHCLRTGWGVIPHAREKSLLPLARA
                     LRRGELVGTAVDQWPARHAVPVTFLNQPTPFASGLFDYAIRTGVPVVAIWAIRTNQNS
                     ATPEYAVTTELVWDREEPVRSADDLVTRWVRMLENVIRCHPDQYLWMHERWKNR"
BASE COUNT         1732 a         2428 c         2287 g         1830 t
ORIGIN      
        1 gaatgacgaa gaaatcattc tccagacgcg attccgagac tttcagcctg gatttctgct
       61 cccgcgggtt ctcgaccatt cgatatacgg agtggcccat cgaaggaacg ccggacacct
      121 caaacaacag ccttttctga ccgtctcttt cagtgacaat ctggctgacg acgggcttcc
      181 ccttctcatc gagcatgccg acagcagcat caccggttat cgcgacactg gcgacgtcgt
      241 ccctatccca gccgagggtg ttgtagatga ctaccggtgt ccctgcaccc gccgtatcga
      301 tgccgcggct tatcgtcgcg agggcctgtt ccctcaaact gctcagtttg cccagaatgg
      361 acgcgtagtc tttgtcggcc tgagtgtata cttcgttcac cgaagaccct ggcagaatgt
      421 cgtgaaactg atttgtgagg aacggtttcc actgctcgta gatctcctgt tgagggtatt
      481 cagcgcctgc gagcatggca acactcgaaa gatattccgt atcacggaat agcagttctc
      541 cctttcgatt gttcctcttt gttcgcgcct gagtggtctg gcaggcacgg tgcagttcca
      601 ggtacaactc gtcgttgtac acagggagct tttctttgtc tacggatttg tgaagccgtt
      661 cgacgtatcc ctcaagagtg ccgaaatcac attccggaag acccgtaaca tctttcattc
      721 tgagaacatt ctcgaacatt tcgatggtaa cgccgccccc tccgtcaccg tgaccaaacg
      781 tgatagacaa ttcgtcaacg acatccttct gtttgacgct tctccagtcg ttcaccgcat
      841 tcgccggagt cggattgctg ttgtaactcc ccggaggcat gaaggcgaga atttcggttc
      901 cgtcgagacc ccgccacagg aaatggctgt aaggaaactg gttgtactgg ctccagttga
      961 ttttggtcgt cccgaaggcc gtcaactggc atttggcaat gatttgcggc agagtaatcg
     1021 gaaaaccgaa cgcatctgga agccacacga gacggggctg aacaccgaat tccttgatga
     1081 agaaccgccg accgtacaga tactggcgaa ccagggcttc tgcacccgcc acgtttgaat
     1141 cctgttcgac ccatccgccg cctgtggctt cccatcggcc ctctttcacg cgctgtttga
     1201 tcccttccca aatggttggg tagttctctt tgatgaactc gtacagctgc ggttgtccct
     1261 gggagaacac gaactgaggg taacgatcca tgtaccgcag cgcgctggaa aatgttcggg
     1321 cgcacttgcg gcgggtttcg cgaagcggcc acaaccatgc ggtgtcgatg tgcgagtggc
     1381 cgacataggt gattttcccg tctccggggc aacgtcggaa atccttgagc tttgcgcgga
     1441 accttttctg agcatcggcg atgtttgccc ggaatgatgc gacatcgtcc ttgttgttga
     1501 ggtcgataag ctttacggcc cagtctacga ggtcctgcag cctcatacgt ggctgcgagt
     1561 ctggcggaag aaccgccacc acatcgtttg cgactttcaa gtcccaatag aaggaccaca
     1621 cgagttcatc gcgctcagcg atttccgcag acgtaaattg atggattgga ttgtcgtcgt
     1681 actggttcgg tgtggcagaa gcttcgacaa cgatttcgaa ttcctctcca cctttggcac
     1741 aggtcgtcag caccacctcc gaacggttcc tgtccaggcc ctgagccggt gcgccattca
     1801 agtagcacag ggcttcccat cccggattga ggatcgccgc gaccgttctt cccgccattt
     1861 cgcgcgggat ttggacacgt gctttgaacc actgggtaac gtcgtatcct ccccagtcat
     1921 cgcccaattc gtacggtatc catccgtcca caggaatggg ggcgggatat tgccccggac
     1981 cggctacgta agtctgccat ccggtaatcg gagtgcgatt gcgatacagc gctgcaaaaa
     2041 gctcgttgag ccggttgccg atttgacgaa cgagaatcgt cctttctgca ggaaaatggg
     2101 gcatgctttc aggtcctttc aaagcgagag tgttgtcatg ttatcgtgaa gacggtcagc
     2161 cgttcgagaa tgtggctttc agtgtcttga tttcccaagg tttcatcatc agttgaacac
     2221 tcgaatcctt gacgggaaca tctacgtcgt gttcttccat caggtcgcat tccgtgacat
     2281 tcaggggcac cttgccgaat tggagcgtag cttcaccccg ggcaccatgc gactcgtaca
     2341 cacgaatgat gatcgcgtcc gagtcctccg ctttcttcac ggtgtcgatc actatgtcag
     2401 acgagtccac ggatacaaag gaccagcgac gtgggtgcgt gcctccgtgg gccttttcta
     2461 tccaacatga gagtggtgca ttcagctcgt acgctcgtcg cgtcgtcaag gcggaacgcc
     2521 agtctccgcg atgagggtac aggctgtaga ggaacaggtg ttgaccttca tccgcgtgag
     2581 gatctggcga gacggatccc cgaaggaggc ttattcgcat gttgttctcg cagacgtcga
     2641 acccgtattt gcaatcgttc atcaggctga cgccatagtc cgcttccgag agatcaatcc
     2701 acctgtgccc cggaacctca aaacgcgccc tttcgaccgg gccagaacgg tgggtcgggc
     2761 gttcgatggc tccgaactga atttcatagg tggcaatgcg cgaaaggacg ttcaccggaa
     2821 aagaggcttt cagcaatttg tgtttctcat accactcgac ttccgtcgca aagtcgatcc
     2881 gcgacagaga tgcccagatc gagatgtcct gtgttatccg agagtttttc gtccttttca
     2941 cgactcgaac ggtggcgcga acgggtcctc gttctgtcac ggttacggac tctgtgtcac
     3001 ctatttcctg aaacttctcc tcaaagttga aatcgatgtc ccacgcgtcg gcgctggctg
     3061 ggaaatcctc aaacagcacc agtctgttcg ctgaagaacc ttccggaacg acttcccgat
     3121 tgttgagttt gtcgaatatg cgcgagattg accccgtttt cgacagggca acttcgaaaa
     3181 accggttttc cagattgttt cgccgggcaa tcaaattccg tctcgccctc ggagcacgct
     3241 tgacgaggcg gaaaacagag taacccatcg gcgggaggtc cgacgcttcg aaaagcaatc
     3301 ccgctgctcc ggattcaagg atcaccggtt gggtggccac ctcgcgcccg tcggggtcca
     3361 gtacactcca tcctgatgaa tcggcggagg gaagcggggt gatggcgaca tcgtccctct
     3421 tccatccgag ggaattccac actatcaacg catcaccttc tccgcgcgtg tctatccggc
     3481 gagcaaggct gcgcaatgag tggtctttga ggttttgaag agctctccgc acaccagcat
     3541 agtctttttc cgcgtcttcg tatacttcgg cgatagacga gcccggcaat atgtcatgga
     3601 actggttcag aagaacggtt ttccacgctt cggagatcgt ctcctgcggg tattcgccgc
     3661 ccgcgatgag ttcacacgaa gccaaaaatt ccgcctcgcg caacgccagt tcacttttgc
     3721 ggttattccg cttcgtccgg ctttgggtag tctggcaacc tcgatgcttt tcgtaataca
     3781 gctcatccac atacacggga aggtcttcgc gatttaccga tgtcatcagc cgatcgaagt
     3841 ggtcctgaaa cctacctgcc cgtgcttgcg gaaacccacg aacatttttg agacgctctg
     3901 cgttttcgag catttccgcg gtggggccac caccgccatc tccgatcccg aaggtcatag
     3961 gaatttcgtc ggtcgacgtt ttctgcttga agcggttcca atgcgcaagg agattggccg
     4021 gggtgacatt ccactcccac ggcatctggt acgcaaggac ttccgatccg tccagtccgc
     4081 gccaccgaaa caggctgtac ggaaactcgt tgaattcgtt cgacgccaat ttcgtcgttg
     4141 cgaacgcttc gacacccgcc ttgcgtaata tctgcggcaa ggcaaacgag aaaccgaaac
     4201 aatccggcaa ccagaaaacg cgagtgtgta caccaaattc gcgtcggaag aagcgattac
     4261 cataaaggaa ctgccgcacg tgcgattcgc cactcgggat attcgtgtcc tgctcgaccc
     4321 acgatgcccc cgtgggttcc cacctgccgg cgcgcacctg ttctttgata cgcgaccaga
     4381 gagaagggta atgggatttg acccactcgt acacctgcgg ctggctctgg aaatagacat
     4441 aatcggggta ctggtccatg taattcagca cggtggaaaa cgtccgcgcg actttccgcc
     4501 gcgtttcccg aagaggccac gtccacgcca catcgatgtg ggattgcccg gcaagaacca
     4561 catttcccat cccgacagac gacgcaaatt cctccatccc cgcggcgaag attttcctta
     4621 ccgacgcaat gtggctgcgg aacgctcgtc ggtcaccggc gctgttcaaa tccacagcct
     4681 tcacgcactt ttccacatgc tcgaggattc gtctgctggt gagcgattcg ttgtcaagcg
     4741 cgttggaagc gtcgaaggca accttcagat cccagtggaa ggaccttatc aactcgtcgc
     4801 ggagcgctac ggcggcaacg gagaaccccc gtcgcccggt attcgagttc gacggcgatg
     4861 agtacgactg gagaaccagt tccacgttct tgccccagaa tcggcgtggc agcacgactt
     4921 cctgcctgtt ggcgtccagg ccctgcaacg gttcgccgtc cgcgagaacg agagactctc
     4981 caccggggtt gagaaggcag acaggacgtt cgccggtcgt tggttcgggt atctcagcac
     5041 gggcgcgaaa ccagcacgta acgtcctgtc caccccacca cgaaccaagc tcgtagggct
     5101 gccaaccggc ttgtgggaca ggggcaacat cctgccgtgt accgacgtcg acatattccc
     5161 atccttcgac cggttggatg gaggagtact ggagcgcaag aatctccccg agcctgttcg
     5221 caatctgccg cgcgacgatg gcgcgctctt gaggaaagtg gatcatacgg aggccgtttc
     5281 ggttgtgctg aacgcatggt ccagcaaagc attaacgcga ggggtaggtt ccttgaaagc
     5341 cagagaagga aatggtgccc cgccacacag cgcaacaggg caaacgggtt caaagccacc
     5401 tgaacgaata tacgtgctac tcgacgcgga cggaatgctc cttcttcgat gttggacgag
     5461 cgagcgcacg ttgtgaaaac ccgtctcaag cccgtataat tgagtgatgt gttgcctcat
     5521 gcggcgcggg ggcatgcctc actaccagaa ctctggatga ggctcgaacc agttcccttt
     5581 ttctgcctcc atttccgcac tggtttccgc gttcgccttt ttttgtttga ctgaagctgt
     5641 tcattgaccc gctaatcccg tatggggaga tagaagcatg gttcttgcga ttgatcaggg
     5701 aacaacaagc tcgcgtgcga tcattttcga cgtttcaggc gcaattctag gacaaggtta
     5761 cgccgaactt ccccagtatt atccccaacc cggatgggtg gaacacgacc cggaggaaat
     5821 ctggtccact gttacggaag ccatcaagca agcgctggtc gcatcgaacg tctcaccggc
     5881 ggaaatcaaa gccgtagggg tcgcaaacca gcgcgaaacg accattatct gggataggag
     5941 aaccgggaaa ccgatccacc gggcaattgt gtggcagtgc cgacgaacgt ctgatatctg
     6001 cgacagactt cgcgccgagg gttgggaaga ggaaatccac aacgccacgg gcctttttat
     6061 cgacccgtac ttcagcgcaa cgaaaatccg gtggttgctg gacaacgtcg agggtgctca
     6121 ggaagcggcg gaaaaggggc acctggcctt tggaacggtc gattcctggc tcctctggaa
     6181 actcagcggc gggcatattc acgcgacaga ctacacaaat gcatcacgaa cacttctttt
     6241 tgacatccgc gcgaaaagct ggaaccagaa gcttctctcc catttgaaga ttccgcgttc
     6301 tcttatgccg caggtcaagt actcctcggt gatgtacggc aaaacaaact gcccggacgt
     6361 gctcccgaaa ccagtcacga tcagcggcgt tgccggtgat cagcaggcat cgttgttcgg
     6421 ccagttcgcg taccagcccg gccagaccaa aacgacgtat ggcacgggtt gctttctcct
     6481 tcagcacacc ggggccgagc cgaaggtgtc cgcacatggc cttcttgcga cactcgcgat
     6541 agacgaaacg ggtggtccgg cttatgccct cgaaggcgct gtcttcaatg cagggtctgc
     6601 ggttcaatgg cttcgcgacg gtcttcaggt gatcacctcc tccgccgaat cggaagcata
     6661 cgcagaacag gtcagtgaca cacttggggt atacgtcgta ccggcgttca atggcctcgg
     6721 tgccccctac tggaacgcgt ccgttcgagg agcgatgttc ggcatcaccc gcggaaccgc
     6781 gcgccaccac atcatccggg caaccctcga atcgattgcc taccagacgg cggatgttct
     6841 ggatgcactg gctgaggaca gcggcattcg gattccagag ctttttgtgg acggcaaagc
     6901 cacccagaac aacttcctca tgcagtttca ggccgacatc gccggtgttc ccgtagttcg
     6961 tcctgtcatg caggaaacga cagtgctggg agcggcactt ctcgccggaa taggcgcagg
     7021 ggtgtggagc agcgcacaac aggcccagga gatcgcacgc cttgaccgcg tgttcgaacc
     7081 ggcgatggga gaggagcaga ggcacgcatt gcggaacggc tggagacgtg cggtagcggc
     7141 ggcaactcag ttctgagttg cgtcggttcc cccgccggcg cttgcctgcg cgggcggaac
     7201 ggcgcgcgca aatgagtccc agattcggcc cgccgcctct tccagatgga cgaggtctcc
     7261 gtcattttgc acgaccatgg ccgcgttttt aatccgctct tcatccggta actgggaact
     7321 gattctccgg gctattgcgt caagatccag gccttttgcc gcgagacgcg cttccacgat
     7381 acctcgcggc gcagtgacga caaccagcgc gtcgaaccaa ctcgtgatgc cccattcgac
     7441 aatgagcgcg gcgtcaacca cgacgagcgg gagcgcgctg tcgaggcgtg cacggtcgag
     7501 ttcttcgcgg agtgtttcca gcaggggtgg atgaacaacg gcgttcagtc gcgcaagtgc
     7561 caccgggtct ccgaacacca gatcaccgag tttcttccgg tcaatggacc cgttttcccc
     7621 gagaatatcc gctccaaacg catcctccag cgccgaacga accttcttgt tcgttttgag
     7681 cacaacgtgg ccgattttgt cggcatctat cacccgtgca ccacgctttg caaggaattt
     7741 cgccaccgtg cttttccccg tcccggttcc cccggtcagg cccagggccg gtgtcatcgc
     7801 gtatctcatc gatttttcca tctttcgtgc atccagaggt actggtcagg gtggcaacgg
     7861 atgacgtttt ccagcatgcg gacccagcgc gtgacgagat catctgcact gcgcaccggc
     7921 tcctcgcggt cccacaccaa ttcagtcgtg actgcgtatt ccggcgtcgc gctgttctga
     7981 ttcgtccgta tcgcccatat ggcgaccacc ggcaccccgg tgcggatggc gtaatcgaac
     8041 aacccggacg caaacggggt tggttggttc agaaatgtta cgggaaccgc atgccgggcg
     8101 ggccactgat cgaccgcagt tccgacgagt tcaccgcgtc tgagggcgcg agcgagcgga
     8161 agaagcgact tttcccgcgc gtgcgggatg acgccccagc ctgtccgaag gcaatgcacc
     8221 aggcgggcaa ccccggggct cgttggtgtc gcgacgataa ggtggagcct gcctccc
//
LOCUS       DAOSOO010000144         8258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8626_length_8258_cov_6.902719, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000144 DAOSOO010000000
VERSION     DAOSOO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8626_length_8258_cov_6.902719"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..199)
                     /locus_tag="PLT86_10170"
     tRNA            complement(113..199)
                     /locus_tag="PLT86_10170"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Tyr,seq:gta)
     gene            complement(235..307)
                     /locus_tag="PLT86_10175"
     tRNA            complement(235..307)
                     /locus_tag="PLT86_10175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Thr,seq:tgt)
     gene            563..1069
                     /locus_tag="PLT86_10180"
     CDS             563..1069
                     /locus_tag="PLT86_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT37018.1"
                     /translation="MRTNRMPGVALIVNCLAIVVIAGVVGCAGSTTTASGLRLKKSAP
                     VTSCNTGRASGAGEVDTVDVEWLMAPLRVLSYADPVYPESALNANQGGEIEARVLINP
                     DGTIGKVVDVTGPEVFRTPAMEAIHGAELTPPTSVYGQPVVVCGTLVVSFQPAHGGLS
                     WLNLRTRQ"
     gene            994..1800
                     /gene="sfsA"
                     /locus_tag="PLT86_10185"
     CDS             994..1800
                     /gene="sfsA"
                     /locus_tag="PLT86_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOT37019.1"
                     /translation="MRNAGGLLPAGPRGIILAQSAHAAVKTVEIPFGPLLEAVFERRP
                     NRYLAWVRLRDGNASESVPVHVPDPGRMRELLLPGRRVWVAPASARANPRTTQFTLAI
                     VESPASAGGAATFVSVNTIIPNKLISRALLDRALPEFEGFTDVRAEVPHGRSRFDFVL
                     TSATASCVLEVKSVSLTHGRTGLFPDAVSARATRHVGELAGLCQRGERAAVLFVAQRN
                     DVDEIRPAEEIDPVFASAMKDAKKAGVDFFGFRCEVSLDGIRLGSPVPVG"
     gene            1955..4822
                     /locus_tag="PLT86_10190"
     CDS             1955..4822
                     /locus_tag="PLT86_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOT37020.1"
                     /translation="MQGYDASESVRVDLHLHSRFSDGLLSPTELAARLSEADVRYASL
                     TDHETIEGLGEFRAAASRLGIVAIPGVELTTLHNGAEVHLLGYGFNVESEELNEVLAR
                     ARVTNNPDSSQFSPSQRLTTSAAIDLIHHAGGSAFVAHPLQTESDPVRLRSLLDELRG
                     SGLDGVEAYYGPFPPDKREEVAEIASGAGLLVCGGSDGHGNGEARMGVDMPLLRWKEF
                     RNALIEGAAQKTQRGPSAQPARPLEERFHWRAFLFHIAMPAFLAVTLFVVALFAFLLP
                     YFERTLIDRKRENIRELTQTAWSVLDEAEREVSAGNLTREQAQTLAIKRVEAMRYGPE
                     GKDYFWLQDLTPRIIMHPYRRDLNGHDVSQFRDPNGIRIFVEFADLVRKQGEGYVSYV
                     WQWKDDPERMEPKESYIRLFAPWGWVIGTGIYVHDVQREIAGLRQRLVNVSLGVTGAV
                     LLLLLYIVRQSVKLERARRDAERRLRESTDRYRALTSAATEGALFVVGRRCAYANPVM
                     SEITGYTQTQLELVDVDDLFPAVEANIEWRRRLDNPGGADTDIPVPGVLQRRDGTRLA
                     CSLSLRRVPESPRGYMILARNVTAASEPPEARALLNRLLLLPEGVASDLTAAIQHATK
                     PADVVGACLRAPKLVRALLENGASPMLVTRVLAELTDEATRRLIFLATEDIGPAPCPY
                     SFLALGSQGRQSQTLYTDQDNALLYELTGSEDPETANRWFLELGQRVCTDLARAGFRD
                     CRGGSMASNPRWCQPLPVWKRYFEEWIRNAEPREVMEFSIFFDFRSVDGDVELSRELR
                     RHVTGVLRTAPHFFAQAAQNALQFKPPLRLFGSIVGGRDREHSGLLDLKSVMMPIVSF
                     ARLYALQQGVEETSTLDRLNALTRLGILLESKRQGILAAYEALLRLRLRNQSDAIELG
                     RPPDNWLDPKTLGRIEEAVLHECFNEIDLLQERIRRDFLAG"
     gene            complement(4880..6502)
                     /locus_tag="PLT86_10195"
     CDS             complement(4880..6502)
                     /locus_tag="PLT86_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOT37021.1"
                     /translation="MMSYPLVLAFIVFFVAVTIVVTWFTQKYTKSTSDFYVAGKKVPW
                     VQTGIAMVGSYLSAASFLGCAGDISIFGVDRIWLSIGFFGGYMAVLMLIAGPLRNVGS
                     YTVADALHRRFPDRRIKLVVMITTVVISTFYLVPQMLGAGLLFEMLMGWDFVTVTAAL
                     GVLMSLYIIFGGMKATIYNQLIQAMFLFFAMTFITVFGFIMYYSGSLDALLGAMANIV
                     PPGICGKDAAAVAAVQSAVSSSAAVSVAREMLPTAPSALTIGIQTPDLVSQISTVVAL
                     VFGTAGLPHILIMFYTVRSASAARKSVTLCIVGLGIFYLCTILLGFILMPQVYPQLVT
                     WIAAGKQGLAKNMAVLQISGKVGGQWLMAVSAAGAVAAILSTSAGLMITVASTISHDF
                     YKSYINPNASERQEVNIAKVTTLVMSVISVVLAVAVKGENVAWLVTLAFGIAASAIFP
                     AMVTTLWWKRLTRQGAIAGMVTGLVVSVLFIVLLLGGVRTFLGLPTAGGPGVFGVTAS
                     FVVLLIVSYRTNDRGPEVEEFFALAHKDEEVE"
     gene            complement(6507..6767)
                     /locus_tag="PLT86_10200"
     CDS             complement(6507..6767)
                     /locus_tag="PLT86_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37022.1"
                     /translation="MLSPEQEKSSPPPGGATGPYRRKAILSFSVFGVFFVFYMGTAIL
                     QTPTFSKLAGIPWFGMPLGLLLSLLIFPVSWALIAVYFRLGR"
     gene            complement(7002..>8258)
                     /locus_tag="PLT86_10205"
     CDS             complement(7002..>8258)
                     /locus_tag="PLT86_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT37023.1"
                     /translation="SEADPDGTWVPSSWSFDLSADDPGNPWQANWTLEQVREYLDAAT
                     YPLIVWDTWSEEAAKYERTDYFYGHPWAFSVLHCFGGETYLLGNVKELIRRAHALGEN
                     PQEVGCVGFNVVPENSDYNSFYFELAARLQWNPRAVDLDEYVQKYCRLRYGPQYVAAT
                     EPVWRLLVETVYGEDSGTVKIIMDPLYWFRPDLRLLAGWPEDDERVIPMWRRRSEFIP
                     KLRRAAELLLAAGNLVGENNMARRDLVDIARQWIAERFNQALIGARDAFLAGNGGELA
                     RLEPICLRLLDDQARLLASWPAYRLSRQVAQVRDEYPDPVKAVKLLHVWCNPVEGQES
                     VPLRDYYRMDLDELVADYYKPRVAAYFALLRDKCAAGRTTVTDEEFDAVYAPVERAFV
                     AAPLCPLPDDEDPTDVVQDLLEDNVE"
BASE COUNT         1757 a         2639 c         2324 g         1538 t
ORIGIN      
        1 cccgcgacat tcagcttgga aggctgacgc tctaccaact gagctattcc cgctcgcgct
       61 gttgtcgttg cggtcggaaa tctcgcatgg accgctcgat atatgaaaaa gatggtgggc
      121 ggtaagggat tcgaaccccc taagccatca ggcaacagat ttacagtctg ccccggctct
      181 ccaactccgg cgcccgccca caaccgtcac tattctatgt atatttcgga aaggagccga
      241 tgaagggatt cgaacccccg acccgcgcat tacaagtgcg ctgctctacc aactgagcta
      301 catcggcata caagagactc ttgaagttaa tagcgcccgt ttcgtttgtc aagaccaccc
      361 cctccgcgct gcatctgcaa gtccattact gcacaacggc ataccattaa atcccatctg
      421 tcaacgggaa caaaaccacc accggctccg tataccgttc aaaaccggac acgcacttgc
      481 acgagtccac atgacggcct atattgctac gtgcactttt ccaccctcac caccgcactt
      541 tgttcgattg gggagacgcg ccatgcgtac gaacaggatg cccggggtcg ctctcatcgt
      601 gaattgcctt gccatcgtcg ttatcgcggg ggtggtcggg tgcgccggat caacaaccac
      661 tgcgtcgggc ctgcgcctga aaaaatccgc tcccgtcact tcctgcaaca ccgggcgagc
      721 ctccggcgca ggggaagtcg acaccgtcga tgtggagtgg ctcatggcgc cgctgcgcgt
      781 gctgagctat gccgaccccg tgtatcccga aagtgccctg aatgcaaacc agggaggtga
      841 aatcgaggcg cgtgtcctca tcaacccgga tggcacaatc ggcaaagtag tagacgtcac
      901 cgggccggaa gtgtttcgca cccccgcgat ggaggcgatc cacggcgcgg aactgacccc
      961 gccgacatcg gtgtacggac agcctgtcgt cgtgtgcgga acgctggtgg tctccttcca
     1021 gccggcccac gggggattat cctggctcaa tctgcgcacg cggcagtaaa aactgtcgag
     1081 attcccttcg gcccgcttct cgaagctgtc ttcgagcggc gccccaaccg ctacctcgcg
     1141 tgggtgcgcc ttcgcgacgg caatgcctcg gaaagcgtac ccgtgcacgt ccctgacccc
     1201 ggccggatgc gggaacttct gcttcccgga cgccgggtgt gggttgcacc ggcttcagca
     1261 cgggcaaacc cccgaaccac gcagttcacc ctggcaattg tggaaagccc cgcatctgca
     1321 ggaggcgcgg ccaccttcgt cagcgtcaac accatcattc cgaacaaact gatatcccgg
     1381 gcccttcttg atcgcgcgct gccggaattc gaagggttca cagacgtgcg agccgaggtg
     1441 cctcacggac gcagtcggtt cgattttgtc cttacgtcgg ctactgcgtc ctgtgtgctc
     1501 gaagtcaagt ccgtcagcct cacacacggg cgaacgggcc tctttcccga tgcagtctcc
     1561 gcccgcgcaa cgcggcacgt tggggagctt gccgggttat gtcaacgcgg ggagagggcg
     1621 gcagtgcttt tcgtcgcgca acgaaatgat gttgacgaga ttcgccccgc agaggaaatc
     1681 gaccctgtgt tcgcttccgc aatgaaagac gcgaagaaag cgggcgtcga tttctttggg
     1741 ttccggtgcg aggtctccct cgacggtatc cgcctcggct cgccagtgcc ggtggggtga
     1801 cccgggtcgc ggaagacgcc atctttctgg aaggaatgtc gccagcggga acggaagagc
     1861 cgacggcttc cccggagagc agaatgccac tctcagctcg gttctcgcgg cgttcgagcc
     1921 cgatctcccc tcgcccgggc aaaagggggt gtcagtgcaa ggctacgatg catctgagtc
     1981 cgttcgggta gacctgcacc ttcattccag gttcagcgac ggtctcctgt cgccgactga
     2041 gctcgcggct cggctttccg aggcggacgt ccggtacgcc tctctgacgg accatgagac
     2101 gatcgaaggg cttggcgaat tccgtgctgc ggcttcgcgg ctcggaatcg tggcgattcc
     2161 cggggtcgaa ctcacgacgt tacacaacgg ggccgaagta cacctcctcg ggtacgggtt
     2221 caacgtcgag tccgaagaac tcaacgaggt ccttgcgcgt gctcgcgtca caaacaaccc
     2281 tgactcctcc cagttctcgc ccagccaacg cctgacaacc tccgcggcga ttgatctcat
     2341 tcaccacgcg ggtggtagtg ccttcgtcgc gcatcccctc cagactgagt ccgaccctgt
     2401 tcggctgcgt tccctcctcg acgagttacg cgggagcggc ctggacggcg ttgaagccta
     2461 ttacggcccg ttcccgcccg acaaacgaga ggaagtcgca gagatcgcct ccggcgccgg
     2521 gctgcttgtc tgcggcgggt cagatggcca cgggaacggc gaagcgagga tgggagttga
     2581 catgccgctt ctccgttgga aggaatttcg gaacgctctc atcgaaggcg ccgcccagaa
     2641 aacgcaacgc ggcccgtcag ctcaaccggc tcggccattg gaggaacgat tccactggcg
     2701 agccttcctg ttccatatcg ccatgccggc cttcctcgcc gtaacgctgt tcgttgtcgc
     2761 ccttttcgca ttccttctgc cctatttcga gcggacgctc atcgatcgca agcgggagaa
     2821 catccgggag cttactcaga ccgcctggag cgttctggat gaagccgaac gggaggtctc
     2881 cgccggcaac ctcacccgcg agcaggcgca gacgctcgca atcaaacgcg tcgaagcgat
     2941 gcggtacggg cccgaaggga aagactactt ctggcttcag gacctcacgc cacggattat
     3001 catgcaccct taccgccgcg acctcaacgg ccatgatgta tcgcagttcc gggatcccaa
     3061 cggcatcagg atattcgtcg aattcgccga tctcgtgcgg aaacagggcg aaggctacgt
     3121 cagctacgtg tggcaatgga aagacgaccc ggagcggatg gagcccaaag agtcctacat
     3181 ccgacttttc gctccgtggg gatgggtgat cggaacagga atctacgtgc acgacgtcca
     3241 gcgcgagatt gcgggtctgc ggcaacggct ggtgaacgtc tctctgggtg tgacaggcgc
     3301 cgtcctgctt ctgctcttgt acattgtccg ccagagcgtc aaactcgaac gcgcccgccg
     3361 cgacgcagag cggcgcctgc gtgaatccac cgaccggtac cgggcgctca cttccgcagc
     3421 caccgagggc gctctgttcg tcgtggggag gcgttgcgcg tacgcgaacc ccgtgatgtc
     3481 ggagataaca gggtacacgc aaacccaact ggaactggtc gatgtcgacg atctcttccc
     3541 cgcagttgag gcgaacatcg aatggagaag gcgcctcgac aacccgggag gcgctgacac
     3601 cgatatccct gtccccggag tgctccagcg ccgcgacggg acccggttgg catgctccct
     3661 cagtttgcgg agggtacctg agagcccgcg cggttatatg attctggcgc gcaacgtcac
     3721 ggcggcttct gaaccgccag aggcccgcgc gctgctcaac cgactgctgc tcctgccaga
     3781 aggcgtcgct tcggatctca cggcggcaat ccagcatgca acgaagcccg cagacgtcgt
     3841 tggcgcctgc ctcagggcgc caaagctggt gcgcgcattg ctggaaaatg gcgcgtcgcc
     3901 catgcttgtc actcgtgttc tcgcggaact caccgacgaa gcgactcgac gcctgatctt
     3961 ccttgcaaca gaagacattg gacctgcccc gtgtccctac tcatttctcg cacttggcag
     4021 ccaggggcgg cagtcacaaa cgctgtacac cgaccaggac aacgctcttc tgtacgaact
     4081 tacaggttct gaagaccccg aaacagccaa tcggtggttc ctcgaactcg ggcagcgcgt
     4141 gtgcacagac ctcgcccgcg cgggattcag ggattgccgg ggcggctcca tggccagcaa
     4201 cccccgttgg tgccagcctc tgccggtctg gaagcgctat ttcgaggaat ggatacggaa
     4261 cgccgaacct cgtgaagtaa tggaattcag catcttcttc gacttccgat cggtggatgg
     4321 cgatgtcgaa ctttcgcggg aactccggcg acacgtaacc ggcgtgttga ggacggcacc
     4381 tcactttttc gctcaggccg cgcagaacgc gcttcagttc aaaccgcctc tgcgcttgtt
     4441 cggatccatt gtcggtggtc gcgaccgtga acacagcggg cttctggacc tcaaatcggt
     4501 gatgatgccg atagtgtctt tcgcgcggct ctacgccctc cagcagggtg tcgaggaaac
     4561 cagcactctg gatcgcctca acgccctcac ccggctgggc attctgctcg aatcaaaacg
     4621 gcagggcatc ctcgcggcgt atgaggcgct cctccgcctc cgattgcgaa atcaatcgga
     4681 cgccatcgaa ctgggaagac caccggacaa ctggctcgat ccaaaaacac tggggcgcat
     4741 cgaagaggcc gtgctgcacg agtgtttcaa cgaaatcgac ctgctccagg agaggattcg
     4801 aagggatttt ctggcggggt aaatgaaaaa agcccgccac ggcaaggacc atggcgggct
     4861 ttcgcgagga ccggaacgat tactcgactt cctcatcctt gtgggcaagg gcgaagaact
     4921 cctccacctc tggccccctg tcattcgtcc tgtagctgac aatcagcagt acgacaaacg
     4981 aagcagtcac gccgaacaca cccgggcctc ccgcagtcgg taacccaagg aacgtccgaa
     5041 ctccgcccag caacaacacg ataaacaaaa ccgagactac gagcccggta accattccgg
     5101 caatcgcccc ctgccgagtc aggcgcttcc accacagtgt cgtaaccatc gcagggaaaa
     5161 tcgcgctggc ggctatacca aatgccagag taaccagcca ggcgacgttc tcgcccttca
     5221 ctgcgaccgc aagcacgact gagatcaccg acatcacgag agttgtgacc ttcgcgatgt
     5281 tcacttcctg tctctcactc gcgttcgggt tgatatagct cttgtagaag tcatgactta
     5341 tcgtcgaagc aaccgtgatc atcaacccgg cggatgtact gagtatcgcg gcgaccgcac
     5401 ccgccgccga aacggccatc aaccattgcc cgccgacttt cccgcttatc tgcaacacgg
     5461 ccatattctt cgccagtcct tgtttgccgg cggcgatcca cgtgaccagt tgaggatata
     5521 cctgaggcat taggatgaac cccagcagga tcgtgcagag gtagaaaatc cccaggccga
     5581 cgatacacag cgtaacgctc tttcgcgccg cgctcgcgga acggacggtg taaaacatga
     5641 taaggatgtg cggcaggccg gccgttccaa aaaccagcgc cactaccgtg ctgatctgag
     5701 aaaccaggtc gggtgtctgg atcccgatcg tcagtgcact cggagcggtg ggcagcatct
     5761 cccgcgcgac actgaccgct gcggaagagg atactgctga ctgaaccgcc gcaaccgcgg
     5821 cggcgtcttt gccgcagatc cccggcggca caatgttcgc catcgcaccc aacagcgcat
     5881 ccagcgaccc gctgtagtac atgatgaagc cgaacaccgt gatgaaggtc atggcgaaaa
     5941 acaggaacat cgcctggata agttgattgt aaatggtcgc tttcatcccg ccgaagatga
     6001 tgtagaggct catcaacacc ccgagggcag cggtgacggt gacgaaatcc caccccatca
     6061 gcatttcaaa cagcaaaccc gcacccagca tctggggaac gagatagaag gtcgagatga
     6121 ccaccgtggt aatcatcacc acaagcttga tgcgccggtc cgggaacctc cggtgaagcg
     6181 catcggccac ggtgtagctg ccgacattcc gaagcggacc cgcaatcagc atcagcaccg
     6241 ccatatagcc gccgaaaaag ccgatcgaaa gccatatccg atcaacaccg aaaatcgaga
     6301 tgtctcccgc gcatccgaga aagctcgcgg cggagaggta gctccccacc atggctatcc
     6361 cggtctgaac ccagggaacc ttcttgcccg ccacgtagaa atccgaagta gatttcgtgt
     6421 atttctgggt aaaccatgtt accacgatcg tcacggcaac gaagaagaca atgaacgcca
     6481 aaaccagcgg gtagctcatc atgttgtcat ctccccagcc tgaagtagac tgcgataagc
     6541 gcccaggaaa ccgggaaaat gagaagcgac aacaacaaac cgagcggcat gccaaaccat
     6601 ggtatccccg cgagcttgct gaacgttggc gtctggagaa tcgccgtccc catgtagaag
     6661 acgaagaata cgccaaaaac ggaaaagctg agtatcgctt tccgccgata cgggccggtc
     6721 gcgcctcccg ggggcggcga cgatttctcc tgttcgggac tcaacatgaa agcactcctt
     6781 ttcccagtgg ggagagttga aggctacgcg gaaaccggaa gaaaggagta tatgacgcgt
     6841 gagttggcgg gtcaagcacg ttgaacacgc ttgcgcgtat gaaacttgtt tcgctgggcg
     6901 gctctttcct gccgggattg cccggcatct tctcccgcag aaaaggctct tccagacggt
     6961 ccgccgcaaa aaaaatagta tcggagccga ttacctttcg gtcactccac attgtcctcc
     7021 aacaaatcct gaaccacatc ggtcggatct tcgtcgtcgg gtaacgggca gagcggcgcc
     7081 gcgacgaagg cacgttccac aggagcatac acggcgtcaa actcctcatc cgtcactgtt
     7141 gtccggcccg ccgcgcactt gtcccgcaac aacgcaaaat aggccgccac ccgtggcttg
     7201 tagtaatcgg cgaccagttc gtcaaggtcc atccggtaat agtcccgcag gggcaccgat
     7261 tcctgccctt ctaccggatt acaccacacg tggaggagtt tgaccgcttt gaccgggtcc
     7321 ggatactcat cgcgcacctg cgcaacctgt cggctgagac gatacgcggg ccagctcgcg
     7381 agaagacgcg cctggtcatc gagcaacctg aggcagatcg gctccagacg cgccagttcc
     7441 ccgccattcc ctgccagaaa cgcatcacgc gcgccaatca gcgcctgatt gaagcgctcc
     7501 gcgatccact ggcgggcaat atccacaagg tcccgccgcg ccatattgtt ctcacccacc
     7561 agattgccgg cggcaaggag aagctcagcc gcgcgacgaa gcttcgggat gaactccgaa
     7621 cggcgtctcc acatcgggat tacccgctca tcatcttccg gccacccggc cagaagcctc
     7681 aggtcggggc ggaaccagta gagtgggtcc atgatgatct tcaccgtacc gctgtcctcg
     7741 ccgtacaccg tctccacaag aagccgccac accggttcgg tcgccgcaac gtactgcggc
     7801 ccgtagcgca acctgcagta cttttgcacg tactcatcca aatccaccgc gcgcgggttc
     7861 cactgcaggc gagccgccag ttcgaagtag aacgaattgt agtctgagtt ctccggcacg
     7921 acgttgaacc ccacgcaccc gacctcctgc gggttctctc cgagtgcgtg cgcgcggcgg
     7981 ataagctcct tcacgttccc cagcagatag gtctccccgc caaaacagtg gagcacgctg
     8041 aatgcccacg gatgcccgta aaagtagtcc gtgcgttcgt actttgccgc ctcttcggac
     8101 caggtatccc acacaatcag cgggtaggtg gctgcgtcca gatattcgcg cacctgttcc
     8161 agagtccagt tggcttgcca cgggttcccc ggatcatccg cgctcaaatc aaaggaccag
     8221 cttgaaggga cccacgtgcc atccggatca gcctcgga
//
LOCUS       DAOSOO010000145         8258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8627_length_8258_cov_5.125686, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000145 DAOSOO010000000
VERSION     DAOSOO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_8258_cov_5.125686"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="PLT86_10210"
     CDS             <1..1909
                     /locus_tag="PLT86_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37024.1"
                     /translation="VLFGAVACALISVLLPTSEYLIGASRMNLSQLPVGAFGLFFTIV
                     LVNIGIGRFFPTWALSPAEVLAVFAMALIGAVMATADLLGWVFAVDAVPYYLATPENR
                     WMDDLWPHLRQWAVVQGPSEELRWAFVGMPSGVAIPWRIWVVPAFWWGTFVGAVAFGS
                     ICLASIMRRQWAEHERLAFPLAQVPLDIMGNPGGRFNIPAMMRKRAFWIGASIPLFVI
                     MWNIVYYFQPQFPRLPIMDEQGIQLGPGFPETLAIKLNMYVVGFAYMVNTNVLFSVWV
                     WYLIVFLQSTILLRVGYTLGGADDLYSSRDALTSWQGFGGFIVFVFWGFWMARHHLRD
                     VWYSVIGKIKANDEHELLPYRWAVPGLVAAALYMGGYLIALGMSWGMVLVYLFGAFVA
                     FYGTTRVIAQTGLVYMQSPLTPTMFTFYAFGTQGVASSAIVGMVGTYSLVVNGRAPLM
                     PGIFHVSYLGAKIGRSGRRMFVALAMALTVAYLIGTIYMIYISYTHGVTTFLSWAYHQ
                     HGEQVYDAVIKKMQARMPVDAGRWSFLGIGALVMAILTFLQYRVPGWPLHPIGFPIAA
                     AGNIRMMFFSIFLTWVIKSILLRVGGVEAYERARPAFLGIIAGYALGVFISFFVDWIW
                     FPGAGHQIHNW"
     gene            complement(2002..4095)
                     /locus_tag="PLT86_10215"
     CDS             complement(2002..4095)
                     /locus_tag="PLT86_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37025.1"
                     /translation="MTSSVSKAVAALFLAVFSIFGIASAGVIRDGVLMVDGKPFFPLG
                     SWNFSITTPEDIARLGMNVSYRGGPSTPEAVEQFRPFMRQCNRLGIQVVPYLSYGGAG
                     VIPWAPEAVRAISRLASEPNLLLWYVGDDITTRHLEGIQQTVSILRQEAPSVPTAADY
                     IAKATPEAKTTFTQFVDIRCQYSYPIPNDSLRSYMRFFDEQREFVGDPLWTWVQNFMW
                     ASTGVSLGLGIEDGPGPIPDPEQVRLLAFAAINRGVRGLLFFPHHELHLQPELAAEVA
                     LTCREIGLVSSHLAAAKTTFDLPTSDPDLNATAFRYGNSTVVSAMLARGHYHRWVDEA
                     VVKNVWIEVPWSGRGSSLPTAVLVATPDAVNCAVSPAQTPGWVRVRVPSLELAGFILL
                     STDSREIAQLRRGVQRITEQLSGLAVAGSIAQTRKVSAAAWSIGFGNLYDAGNLVLPA
                     VRLNEQAMDAVKEGDEVAEVRLWREANRVCRTVLDSMMVFAEARRALVPAAQQRYLNS
                     PYGLYAIKNLMRAPAVDDPWHHVAQWEVTGPFPLEYDQNAPDVFPPGFERVYPPENAT
                     ASSGPFATVDGPASWKPAVADITGITDFLNYFSTTENVVCYARTTVTAPRDTTMEMSI
                     GSNDGAKVWVNGTEVLSIHPPGGRAAAPHQNKVTVNLRAGRNVVLAKVENLGLNWQLY
                     LSFRDENRVLKFSTD"
     gene            complement(4195..5580)
                     /locus_tag="PLT86_10220"
     CDS             complement(4195..5580)
                     /locus_tag="PLT86_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37026.1"
                     /translation="MPEEPTSLLGRHRFVVSLSTVLGRSLSPDYGNGIVGESYAAGLG
                     EGATVGWDYGVTGVWALGAGQTLRWVNPNRFYRVSPSPKIGAMALSHAWERGSWSIGG
                     TAKRHSFSLTDDFQFVRFPRSSNVQINSFFYDHLETAIGRTISYDALGNNLMFVGDTR
                     WKTRFGTFGVMLSAHNASLDAVTEHRNTIADTLYSRHLLNGIKNGDLNGTWSGTGATL
                     AWDGTFRPGWSYGFRCGESQTSGDAVMWIRNPSEFPDGHGYTYKLEPRVWGLDTLSMK
                     NRHFSLRTSFDPAVSTGIECLFSWQGGSMANGAFGQTPVLTIDAVGDTVLPVSQGAFA
                     TVQGSFEGISILATIRHTPGNEPWSVEGSVGYVRVSAHADFSAVPRTTGFDLSSRATT
                     WDLKAARFGYCAFVFTRRVTSQFYLTYSVRHLFPFSMDWHHNGARDSAPGGLTGFRFG
                     SLHEMSARMVL"
     gene            5897..7189
                     /locus_tag="PLT86_10225"
     CDS             5897..7189
                     /locus_tag="PLT86_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT37027.1"
                     /translation="MNVLLVAIDTLRADHLSCYGWKRLTSPHLDAFAAGGTVFLDMVS
                     PHIPTHPGYTTMFSGHDAMSHQVISHGGNVGPATGVKMIAEILGEQGYFTGAADNLGR
                     WFERGFQDYRRYSWVSNGTEPQRKAEAVNDVALPLLQDAANQDKPFFLFLHYWDPHTP
                     YLPPPPFSRMFFEGDEKDPADRSMDEVFAFAPFEKYFRAWLPGVTSREFVAAQYDAEI
                     AYNDAVLAHVFCRLRELNLEGETLVIVTSDHGETMDEHGCFFDHHGLYDANVCVPLIL
                     QCPGKIPAGQRLRGQVATFDIAPTILDYLGLGDLIAENAMIGTSLRPLAERGSQKGNY
                     DALYVAECSWMRKHGIRTNDWKYIEALEPDFHGLPPVELYDLNEKPVKETTNLVDARP
                     DVVADLKGVLEDWITRRPAQTGRPNPIMEQGITMRSVG"
     gene            complement(7279..>8258)
                     /locus_tag="PLT86_10230"
     CDS             complement(7279..>8258)
                     /locus_tag="PLT86_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37028.1"
                     /translation="FMTDYMSAAVGVFRPDSNSVDVLPLAGVHTDVALRSYGGLVYVL
                     ERFGKDAITVIDPALPTAPTANYSTGNGSNPQDIIVADAHRAYVLRLNTSNVLIINPQ
                     TGDSLGTIDLAPYADPADGRPEMAAGVLESGTLYVLLQLLDTASNPWLWPPTGFGKVV
                     AIDTRTNAVTNAFTLTVKNPVAMAVHNGTLFVAGGPYDDISATGIDRVPLSGGTPTRL
                     VDGASLGGRPTALEVCEQNNSAWVLVQKTWPTATVYRVSLTTGVTADSLAEPATPAAI
                     AVGMDSVLLVSDRSMSVPGLFVYNAITGEKRRGPIATALPPDAIVFVKD"
BASE COUNT         1672 a         2327 c         2480 g         1779 t
ORIGIN      
        1 ggtgttgttt ggcgccgtgg catgcgcgtt gataagcgtt cttcttccca cgtcagagta
       61 cttgatcggc gcttcccgca tgaacctgag ccaacttccc gttggcgcgt tcggtctttt
      121 tttcacgatt gttctggtga atatcgggat agggcgcttc tttccgacat gggctctgtc
      181 accggccgag gttctcgcgg ttttcgcgat ggcgttgatc ggggccgtga tggcgacggc
      241 cgatcttctc gggtgggtgt ttgcggtaga tgcagttccg tactacctcg ccacgccgga
      301 aaatcggtgg atggatgatc tctggccgca tctcaggcaa tgggcggtgg ttcaggggcc
      361 cagcgaggaa ctacggtggg ccttcgtggg aatgcccagc ggagtagcca ttccctggcg
      421 catctgggtg gttccggcat tctggtgggg cactttcgtc ggtgccgttg cattcgggtc
      481 aatttgtctt gcctctatca tgcgcaggca atgggccgaa cacgagcgtc ttgcgtttcc
      541 actcgcgcag gtgcccctcg acatcatggg gaacccgggc gggcggttca acataccggc
      601 gatgatgagg aaacgcgcgt tctggatagg ggcgagcatc ccgctgttcg tgatcatgtg
      661 gaacatcgtc tactacttcc agccgcagtt tccccgcctg ccaatcatgg atgagcaagg
      721 cattcaactt ggcccgggct tccctgaaac cttggcgatc aaactgaaca tgtacgtcgt
      781 tggttttgcg tacatggtaa acacgaacgt tctgttcagc gtctgggtat ggtatctcat
      841 cgtgttcctc cagagcacga ttttgcttcg agtcggatac acgttagggg gtgcggacga
      901 tctctattca tcgagggacg cgctgacgag ctggcagggt tttggtggat tcattgtctt
      961 cgtgttctgg ggattctgga tggcgcgcca ccatctgcgc gatgtctggt actccgttat
     1021 cggcaagatc aaggcgaacg atgagcacga gctgcttccg taccgctggg ctgtgccggg
     1081 cctggtcgcg gccgcgttgt acatgggagg atacctgatc gccctcggca tgtcgtgggg
     1141 tatggtgctg gtctatctgt tcggtgcgtt cgtcgcgttc tacggaacga cgcgcgtaat
     1201 tgcccagacc ggactggtat acatgcaaag ccctctcacg ccgacgatgt tcacgttcta
     1261 tgcttttgga acgcaggggg ttgcgtcctc agcgatcgtc gggatggtcg gcacatattc
     1321 gctcgttgtg aatgggcgtg cgccacttat gccagggatc ttccatgtga gttatcttgg
     1381 cgcgaagatc gggcgcagtg gccggcgaat gtttgtggcg cttgcgatgg cgcttaccgt
     1441 tgcctacctg atcggtacga tctacatgat ttacatcagc tacacgcacg gcgtgacaac
     1501 gttcctctcc tgggcgtacc accagcacgg agagcaggtt tacgacgcgg taatcaagaa
     1561 aatgcaggcg cggatgccgg tagacgcggg gcgatggtct ttccttggca tcggcgcgct
     1621 tgtcatggca atattgacgt tccttcagta tcgtgttccg ggttggccgc tgcatccgat
     1681 cggttttcca atcgccgcgg ccggcaacat caggatgatg ttcttcagca tttttctgac
     1741 gtgggtgata aaaagcatac tgctccgggt cggtggcgtg gaagcatacg agcgagcgcg
     1801 ccctgcgttc cttggcatta tcgccggcta tgcactgggg gtattcatca gcttcttcgt
     1861 ggattggatc tggttccccg gcgcgggaca ccagatacac aactggtgag cacccgtggg
     1921 gatgcatagg aggaataaaa ccggcgtgtg gcgttgaaga gccacacgcc ggttcctttt
     1981 gggaggatcg gaatggcttg ctcagtccgt cgagaacttg agcacgcggt tttcatcgcg
     2041 gaatgacagg tagagctgcc agttaagccc caggttctca acctttgcga gcaccacgtt
     2101 gcgtcctgcg cgcaggttga cggtcacttt gttctgatgt ggtgcagcgg ctcggccgcc
     2161 ggggggatga atcgaaagca cttccgtccc gttgacccac accttcgccc cgtcgttgct
     2221 gccgatgctc atctccatcg tcgtgtcacg tggcgcagtc accgtggttc tcgcgtagca
     2281 aacgacattt tccgtggtgc tgaaatagtt caggaaatcc gttattcctg tgatgtctgc
     2341 cacggcgggt ttccatgatg cgggaccgtc cacagttgca aacggtccgc tggacgcggt
     2401 agcgttttca ggtggataca ccctctcgaa ccctggaggg aaaacatccg gcgcgttctg
     2461 gtcatattcg agcgggaacg gccctgttac ttcccattgc gcaacgtgat gccacggatc
     2521 gtccaccgca ggcgcacgca tgagattctt gatcgcatac agcccgtacg ggctgttgag
     2581 gtaccgctgt tgcgccgccg gcaccagcgc tcgacgtgct tctgcgaaca ccatcatgct
     2641 gtcgagaaca gtcctgcaga ctcggtttgc ttctctccac agacgcactt cggcgacttc
     2701 atcaccttct ttgactgcat ccatcgcttg ttcgttgaga cggactgcag gcagaaccag
     2761 attccccgcg tcataaaggt ttccgaaacc gattgaccac gccgccgccg agacttttct
     2821 ggtctgagcg atagatcccg cgaccgcgag ccccgagagc tgttccgtga tgcgctgcac
     2881 gccgcggcgg agttgcgcga tctcgcgcga atcggttgac agcaggataa acccggcgag
     2941 ttcaagggat ggcaccctga ccctcaccca gccgggtgtc tgggcaggtg aaaccgcgca
     3001 attcaccgcg tcaggggtgg cgaccagtac ggccgttggg agcgaagacc ctcttccgct
     3061 ccaaggcact tcgatccaga cgtttttcac gaccgcttcg tctacccaac gatgatagtg
     3121 cccgcgggca agcattgcgg agacgaccgt ggaattgccg taccggaatg cggttgcatt
     3181 gagatccggg tctgaggtgg ggagatcgaa cgtcgttttt gcggcagcca ggtgggacga
     3241 cacaagtcca atttcacggc acgtcaaggc tacttccgcg gcgagctccg gctgcaggtg
     3301 aagttcgtgg tgcgggaaga acagaaggcc gcgcaccccg cgattgatcg cagcgaacgc
     3361 aagaagtcgc acctgctcgg ggtccggaat gggccccggt ccgtcttcta tgccaagtcc
     3421 cagcgagact ccggtgctcg cccacatgaa gttctgcacc catgtccaga gtgggtctcc
     3481 cacgaactcc cgctgctcgt caaagaaccg catgtacgac cgcagggagt catttgggat
     3541 ggggtaggaa tactggcacc gaatgtccac gaattgcgtg aatgtggtct tggcttccgg
     3601 tgtcgccttg gctatgtaat ctgccgcggt tggcaccgaa ggtgcttcct gtcgcaagat
     3661 agacaccgtc tgctggatgc cctcgaggtg tcgcgtcgta atatcgtcac ccacgtacca
     3721 gagcaaaaga ttcggttcac tcgccagtct ggaaatcgct cggacagctt ccggcgccca
     3781 tgggatgacg cctgcgccgc cgtacgagag gtatggtact acctggatgc ccagcctgtt
     3841 gcactggcgc atgaaaggcc ggaactgctc caccgcctcg ggagttgatg ggccgcctcg
     3901 gtaacttacg ttcataccga gacgcgcgat atcctcgggt gttgtaatcg aaaagttcca
     3961 gcttccgagg gggaagaacg gttttccgtc caccatcagc acgccgtctc gaatgacgcc
     4021 cgccgaggcg atgccaaata tggaaaatac ggcaagaaac agcgctgcga ctgcctttga
     4081 taccgaactc gtcattggac tctccttgat tgccatggga ggaaacctga ggggcaattg
     4141 aaatgtcgcg agggagctcc aaacgcccca agggaggggg ccgacctggg ccgatcaaag
     4201 caccatgcgt gcggacattt cgtgcaggct tccgaatcgg aaaccggtca ggccgccagg
     4261 tgccgaatca cgcgccccgt tgtgatgcca atccatggaa aatgggaaca aatggcgcac
     4321 ggaatatgtg aggtagaatt ggctggttac gcggcgggtg aaaacgaacg cgcaataacc
     4381 aaaacgcgca gccttcaggt cccatgtggt cgcacgcgag gacaaatcga atccggttgt
     4441 gcggggaacc gcggaaaaat cggcatgcgc ggaaacgcga acatacccga cacttccttc
     4501 gacactccac ggttcgttgc cgggagtatg ccgaatggtg gcaagtatcg aaatgccttc
     4561 aaatgagccc tgaacggtcg cgaaggcccc ctgactgaca ggcaggacgg tgtcgcctac
     4621 ggcgtcgata gtaagcacgg gtgtctggcc gaacgctcca tttgccatgg aacctccctg
     4681 ccagctgaac aggcactcaa tcccggtcga cactgcagga tcaaacgacg tgcgcagcga
     4741 gaaatgcctg ttcttcatcg acagcgtatc caacccccac acccggggtt cgagtttgta
     4801 cgtgtaccca tgtccgtcgg ggaactcaga cgggtttcgg atccacatca ccgcgtcacc
     4861 ggaggtctgg ctttcgccgc accggaaacc gtacgaccaa ccaggccgga acgtaccatc
     4921 ccaggcaagt gtggcgccgg tgccgctcca tgttccgttc agatcaccgt tttttatgcc
     4981 gttcagcagg tgccgggaat agagggtgtc tgcaatcgtg tttctgtgtt ccgtgacggc
     5041 atccaaactt gcgttgtgtg cggacagcat gacgccgaag gtaccgaaac gtgttttcca
     5101 gcgcgtgtcg ccaacgaaca tcaggttgtt gccgagcgcg tcatacgaga ttgttcgtcc
     5161 aattgctgtt tcgaggtgat cgtagaagaa actgttgatc tggacattgg acgagcgtgg
     5221 gaaacgcaca aactggaagt catccgtaag actgaacgag tgtcgtttcg cggtgccgcc
     5281 gatagaccag gaaccgcgtt cccacgcatg ggagagcgcc atcgctccaa ttttcggtga
     5341 gggagacacg cggtagaaac ggttcggatt gacccaccgg agagtttgtc cggcaccgag
     5401 cgcccagacg ccagttaccc cgtagtccca gcccacggtg gcgccttccc cgagacccgc
     5461 ggcgtacgac tccccgacaa tcccgttacc gtaatcgggg gaaagagaac gcccgagtac
     5521 ggtcgagagt gatacgacga accgatgcct gcccaggagg ctggtcggtt cttccggcat
     5581 ccacaactcg cttcggggac tcgtccggac gccaccaagc ccatcaacga gcaggttcat
     5641 tggaccgagg ccggcggaag tttttgtaac tccaaccaac accgggaaaa gtaacaggag
     5701 tgttccgcgc acgcgttcag accccgccat ccagctgaca ctattttgac gattccaaat
     5761 gtacgtcggt gggtttctgc gtgcattccg cggccataac ccgttgactc gagtccacgg
     5821 cggacgtaaa tcgtacacgc gggaaatcgc gcgagatccc cgggttgaaa gtgcgcaaat
     5881 gtcgaaagga gcgactatga acgttcttct tgtagcgatc gatactctcc gagccgatca
     5941 tctaagttgc tatggctgga agaggcttac cagtccccac cttgatgcat ttgcggccgg
     6001 gggcacggtg ttccttgata tggtgtcgcc ccatattccg acgcacccgg gttacacgac
     6061 gatgttttcg ggccacgacg ccatgtcaca tcaggttatt tcccatggcg gaaacgtggg
     6121 acctgcgacc ggcgtgaaaa tgattgctga gatactcggc gagcaggggt atttcaccgg
     6181 tgccgcggac aatctgggcc gctggttcga acggggattc caagattacc gcaggtactc
     6241 ctgggtatcg aacggaactg aaccgcaacg taaagccgag gcagtgaatg acgtggcgct
     6301 tccgctcctt caggatgctg cgaaccagga caaacccttt ttccttttcc tccattactg
     6361 ggacccccac acaccctacc tgcctccccc tccattctcg cgcatgtttt tcgaaggcga
     6421 cgagaaggac cctgcggacc ggtccatgga cgaggtgttt gcgttcgcac cttttgagaa
     6481 atacttccgt gcgtggttgc cgggagtaac aagtcgggag tttgtcgctg cgcaatacga
     6541 cgcagagatc gcatacaacg atgcggtact tgctcatgtg ttttgccggc tacgtgaact
     6601 gaatcttgag ggggagacgc ttgttatcgt gacgtccgac catggcgaga caatggacga
     6661 acatgggtgt ttcttcgacc atcacgggct gtatgacgcg aatgtatgtg ttccactcat
     6721 tcttcaatgt cccggcaaga ttcccgccgg ccaacgcttg cgagggcagg tggccacgtt
     6781 tgacatcgcg cccacgatac tggactacct tgggctgggg gatttgatcg cggaaaacgc
     6841 aatgatcgga accagtctgc gacctcttgc agagaggggc tcccagaagg ggaactacga
     6901 cgctctgtac gtagctgagt gctcatggat gcggaaacac gggatccgga caaacgactg
     6961 gaaatacatt gaggctctgg aacccgactt ccatgggctt ccgccggtag agctttacga
     7021 tttgaacgag aaaccagtga aagagaccac gaatctggtg gatgcacggc cagacgttgt
     7081 tgctgacctg aagggagttc ttgaggactg gatcacgcgt cgcccggcgc agacaggaag
     7141 accgaatccg atcatggaac aggggatcac catgaggtca gttggatagg tggacgggca
     7201 gggccggaag cggttgcgtg gagatgcgag gcacatgcat cgttccgctc ccggctcgcc
     7261 cgttttcata accaatcatt aatctttgac aaaaacgatc gcgtccggcg gcagcgccgt
     7321 ggcaatcgga cctcgtcgtt tctctccagt gatggcgttg tacacgaaaa gccccggaac
     7381 actcattgag cgatcgctta ccagcaacac gctgtccatt cctacggcga tggcggccgg
     7441 ggtcgccggt tcggcgaggc tatccgccgt gacaccggtg gtcaggctca cccgatacac
     7501 ggtagccgtg ggccaggttt tctgaaccag tacccacgcg gagttgttct gttcgcacac
     7561 ctccagtgcg gttggtctcc ccccgaggga agcgccgtcc acaaggcggg tcggcgtacc
     7621 tccggataaa ggtaccctgt caattccggt tgcgctgatg tcgtcgtacg ggccaccggc
     7681 cacgaaaagc gtgccattgt gcacggccat cgccacaggg tttttcaccg tcagcgtgaa
     7741 cgcattcgtt accgcattgg tacgagtatc aatggcaacg actttcccga agccggttgg
     7801 aggccacagc caggggttcg aggccgtgtc aagcaactgg agcagaacgt acagagttcc
     7861 gctttcgagg actcccgccg ccatctcggg ccggccgtct gcggggtccg cgtacggcgc
     7921 aagatcaatg gtcccgaggc tgtcccccgt ctggggattg atgatgagga cgttgcttgt
     7981 attgaggcgc agaacgtacg ccctgtgtgc atccgcaaca atgatatcct ggggattgga
     8041 accgttcccg gtgctgtaat ttgctgtcgg tgccgttggc aacgcggggt cgatgacagt
     8101 gatcgcgtcc ttaccgaaac gttccagcac atacaccagt ccgccatacg accgaagtgc
     8161 gacatcggta tgcacgccgg caagggggag gacgtcgacg gagttcgaat caggccgaaa
     8221 aacaccaacc gcggcggaca tataatcggt catgaaaa
//
LOCUS       DAOSOO010000146         8245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8642_length_8245_cov_5.333455, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000146 DAOSOO010000000
VERSION     DAOSOO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8642_length_8245_cov_5.333455"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /locus_tag="PLT86_10235"
     CDS             <1..2998
                     /locus_tag="PLT86_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT37029.1"
                     /translation="PIGNRFLTSVSLAEDASRRVPVLILGGSSVSAREEASRVAGEIR
                     AWRVHLPAEAVETGILQPLDNAGLAVLNRGTIAASAEPGGELFLALQHAAGWCDWATP
                     HYLGFPFVPERKSTVHHYAILPFAGSWSEARIPEEAQAYNRPFTPAQTGIHEGELPSK
                     GQFVNLQGESLAVSAVKPAGQPSAQFLRRNVTAADRLVMRVWNWRGVYCPAGLSLEAG
                     LESAAMVDLAERNPHPIPVCDGAVKFCVGPNAIETLLLTPARFKEQTSLSTRGTARSP
                     LVESRWWRQNLGAAPRGNTPVSVGLRGDLLPGERGNVVLSIAHNRLGESFSGAVRLYG
                     PSGWTISPTLVPVTLGPRSSTAFDINLAVPQDTPGRLVCELAYEGNLYVDSLLCGNAV
                     QPEVQADQRGDGFVVRVSNPSRDTLPISVDLITPLDTWAELDAFALSPIPHQTAHFTL
                     APGEQREAVFAAGAHLIGDFWAFAKVVARESCQYLRLPVRRNATLHAEAGTSFCLNAP
                     LSDCALLGTVSSNSKLAFSVVARQQPAAGIAPSPIPMAGYWSLSPVEGAEHVQAPRLR
                     FRINPEHGRKLHDAATLSMATRRGSAWVPVPTTFDRSRWELLAEVSPETLRERTDWTI
                     FAPVNELWRVSAGRRFFEAHPEVADIDGDGEPEVVIGTAGNGLCALSSRGRILWRRKF
                     GGWMWQSPNTTLADVDGNGNLVIVVAPSDRSLVLLDAAGHVIWRLDDLPFAVKQKPLV
                     ADINGDGRNEILVSIDDNGVFAFDCNGKRLWNCAMEPVRLSAPVMAGGTAGAPEILVN
                     AGGRALAVLNANGALVRMTRLSAGWARDSYVADPVSTDLLTPGTPNILFAGHDGFLRL
                     LAADGTQVWECDLGSPVARAPVVLPAEGGAPPVIVCGAARQTLCGITPVGSIVWRTTP
                     RAHVQSPLGCADVDGDGHLEIILGCGRDQRTVIFDREGNEKASIPYSAIWTNTAVPLG
                     NSSPGRVSVLVTGDWNGDIVAATPHI"
     gene            3162..3965
                     /locus_tag="PLT86_10240"
     CDS             3162..3965
                     /locus_tag="PLT86_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT37030.1"
                     /translation="MKLGCSTILYGVHSTEDALSGIKKAGYDGIELCAIPGMGFHIEP
                     NKTEKEYRELKKKIDDHGLIVESIGGSGNDPYDPDPDSKFRSLMRAAAIIGAPAITTG
                     SGGKSDNQEDMKRAAEVFNKLAEFGKTVGTKVSIKPHVGGVVYNTPSSLEFMSLVDTK
                     WIGLNVDASHLWRAPKWEAGEESIPKLAKYIFTARIRDTLNHDQPIGPVETQVPGGGA
                     MNLKAIMDAFKKVPGLDIVTVEIVGTAQWELSAIQDVVERTYAALKKLV"
     gene            4077..5054
                     /locus_tag="PLT86_10245"
     CDS             4077..5054
                     /locus_tag="PLT86_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37031.1"
                     /translation="MVRTVVLGLDEVGREYARASKRDSASNLVGVYDPDRARAHRLGK
                     ELGVPIFDRPEELLEVAKPQVCVLSALEGKETLASDLLSRGIHVLSTLPVAPDAEQCE
                     KLAARDSEKNGILAADFHLRFTPAMDKAREWVSGGEIGSPLFINMNLWRDVPDRHGDN
                     PYHLFVSLGAHGVDVIRQFVGGVERVQCFGVRDKQGIWTSAQINMMCSNGAVGHLTIS
                     YEMVTHHPIARCEIAGSRARVVIENVYEDATLYVHERPEKLVLTNSIFGGIPQLSETW
                     FRRLHEFLTSVQKGNLARDTTAVDACAAARVMEAAYHSAERGSVEEVES"
     gene            5054..6106
                     /locus_tag="PLT86_10250"
     CDS             5054..6106
                     /locus_tag="PLT86_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37032.1"
                     /translation="MLRTCVIGLGPIGNRHADIHQADPLATLVGVCDWNPERAEAAGK
                     RLGVPFFTDAAKMLESLRPDMVSVATGGHEYGSEHAAATMVALEHGCHVLCEKPISNE
                     LEPARKMVALAREKRLCFAVNLNHRFTPACRHARKWLAEGRIGIPRFMNMAMWIGNPR
                     ESSPWFHFKALHPHTIDIMRYFCGEIEAVHAFAIKAPGRSIWSTAQFNFRFKSGATGH
                     LTGSYDIMRGHPMERAEVGGTRGRILLEDMFKELTLFPHDSSEKIVVTNPIIGGMGDF
                     TDTFINRIHNFFVELTGGVPHDRIDGQAADALAAQAVIDANIRSMRTGKVERVDPESW
                     TGNYDLSYGKPGHSPG"
     gene            6122..>6699
                     /locus_tag="PLT86_10255"
     CDS             6122..>6699
                     /locus_tag="PLT86_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37033.1"
                     /translation="MGISICMVGVGAFAQSFIPLFKAHPLVDAISLCDLDERKLRENS
                     QRYGIPRTYASLDEACDSDVDAVAIITQNWMHAEQAIQALNAGKHVYSAVPTGITVDE
                     VVRLVRTVENTGKVYMLGETSYYYPAVIYCREQFRKGAFGRVVYGEGEYYHDWDHGLY
                     DVMKWRGGERWREFAGGPPMHYPTHSTSQIISV"
     assembly_gap    6700..6799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6800..7081
                     /locus_tag="PLT86_10260"
     CDS             <6800..7081
                     /locus_tag="PLT86_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37034.1"
                     /translation="VVTSQDGTHLHASKVHDVARLPRQFIGLPNGHCGSHQFLVDDFV
                     RACANRTVPPNNVWQAARYALPGIIAHESAMQGGVLLEVPDCGDPPREG"
     gene            complement(7147..>8245)
                     /locus_tag="PLT86_10265"
     CDS             complement(7147..>8245)
                     /locus_tag="PLT86_10265"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT37035.1"
                     /translation="ETWEGYAMKSTKYGILVLGAVLAWSLPVLAAGEFDKLGQSGMTF
                     LKIGPVARAAAMGDAYTGVSTGATGCFYNPAGLGFLTGRVDAMAGQVSWIADIKIASA
                     AAGVPIELGGNRFAVVGISYVGMDYGTIHGTVINKGSTNGAYSDIGNLEPSEFLLGLT
                     LAKQFTDKFSLGLTGKYVNQGLPWYRFGNATYAVPPPDNLGAVPTVGDSLSEFTARNF
                     VFDAGTLYRTGFGSTVVSMSIRNFSRKTTHVIDAFTTPMTFHIGVAGNAFDPIPALGQ
                     MGHKLLIAVDGIHPPDHPEKIALGGEYSFRDMVFVRGGYTFNNDARTWNTGFGVKYGY
                     AGLAGAFDYGYADMGGSLGSVNYFTISFAMQ"
BASE COUNT         1673 a         2496 c         2439 g         1537 t
ORIGIN      
        1 gccgattggg aaccggtttc ttaccagcgt gagtctcgcg gaagacgctt ctcggagggt
       61 tccggtcctt atcctcggcg gctcctccgt cagtgcgcgg gaggaggctt cgcgggttgc
      121 cggagagata cgtgcttggc gcgttcatct gccggcggaa gccgtcgaaa cgggcattct
      181 ccaacctctg gacaacgccg ggctggcggt gctgaaccgg ggcacgattg cagcgagcgc
      241 ggaaccggga ggcgaattgt tccttgcgct gcagcacgcg gccgggtggt gcgactgggc
      301 aacgccgcac tacctcggct ttcccttcgt cccggaacga aagagcactg tacaccatta
      361 cgcaatcctc ccgtttgcgg gctcgtggag cgaggcacga attccggaag aggcacaggc
      421 gtacaaccgg cctttcacac cagcgcagac cggtatccat gagggggagc ttccttcaaa
      481 aggccagttc gtcaatcttc aaggcgaatc acttgcagtc tccgccgtca aacctgcagg
      541 tcaacccagc gcgcagttcc tgcgaaggaa cgtgaccgca gcggacaggc tggtgatgcg
      601 cgtctggaac tggcgcgggg tatattgccc cgcaggactc tccctcgaag ccggcctcga
      661 atcggctgcc atggtcgacc tcgcagaacg gaatccccat ccgattcccg tgtgcgacgg
      721 tgccgtcaag ttctgtgtcg gccccaacgc gatagaaact ctgctgctca cccccgcgcg
      781 attcaaggag cagacttcgc tgagcacccg ggggacggcc agatcgccac tcgtggaatc
      841 ccggtggtgg cggcagaacc tcggggccgc accccgtgga aacacgcccg tatccgtggg
      901 ccttcggggt gatcttctcc ccggtgaacg cggcaacgtt gtgctgtcca tcgctcacaa
      961 tcgactcggg gaatccttct ccggggcagt ccggctttac gggccttccg gttggactat
     1021 aagcccgacc ctggtgcccg tcacactggg cccgaggtcc agtactgctt ttgacatcaa
     1081 cctggccgta ccgcaggaca caccggggcg cctcgtgtgt gaactggcgt acgaaggcaa
     1141 cctgtacgtc gacagccttc tctgtgggaa cgccgtgcaa ccagaggtgc aggccgatca
     1201 acggggcgac gggttcgtag tgcgcgtgtc aaacccctct cgcgacactc ttcccatttc
     1261 ggtggatctt attaccccgc tggatacatg ggcggaactg gacgcgtttg ccctgagccc
     1321 cattcctcac cagaccgcgc atttcaccct tgcgcccggg gaacagcggg aggcggtgtt
     1381 tgccgccggc gcgcacctta tcggcgattt ctgggcattt gcgaaggttg ttgcaaggga
     1441 atcatgccag tacctccgtc tgccggtccg cagaaacgcc accctgcacg ctgaggcagg
     1501 taccagtttc tgtctcaatg ctcccctttc ggactgcgca ctcctcggta ccgtttcctc
     1561 caacagcaaa ctggctttca gtgtggtggc ccggcaacaa ccggctgctg gaattgctcc
     1621 ttccccgatc ccaatggccg gatactggag cctttctccc gtggaaggcg ccgaacacgt
     1681 ccaggcgccg cgtttgcggt tccgaattaa cccggaacac ggacggaagc tccatgacgc
     1741 ggcgacgctt tcgatggcca cacggcgagg cagtgcatgg gttcccgtgc cgacgacatt
     1801 cgaccgttcc cggtgggaac tgcttgccga agtgtcgccg gaaacactca gagaacgtac
     1861 cgactggacg attttcgcgc ctgtcaacga actctggcgc gtatccgcgg gacgccggtt
     1921 ctttgaggcg caccccgagg tcgccgacat agacggagac ggggagccgg aggtcgtcat
     1981 tggcacggcg ggaaacggcc tgtgcgctct ttcctcccgg ggccggatcc tgtggcgccg
     2041 gaagttcggt ggatggatgt ggcagtcccc caacaccact ctcgcagacg ttgacgggaa
     2101 cggcaacctt gtaatcgtgg tcgcgccctc cgaccgctct cttgtccttc ttgacgctgc
     2161 cgggcacgtc atctggcggc tcgacgatct gccgttcgcg gtgaagcaga aaccgcttgt
     2221 cgcagatatc aacggggatg gcaggaacga aatcctcgtg tcgatcgatg ataacggcgt
     2281 gttcgcgttc gactgcaacg gaaaacgtct ctggaactgc gcaatggagc cggtgagact
     2341 gtcggccccc gtcatggccg gcggtacggc cggcgcgccc gagattctcg tcaacgcggg
     2401 aggcagagcc ctggcggtac tgaacgccaa cggtgcgctc gtccggatga cgcggctctc
     2461 ggccgggtgg gcgcgcgact cgtatgttgc cgatccagtc tctaccgatc tgctcacacc
     2521 gggtacgccg aacatcctgt ttgccggaca tgatggattc cttcgccttc tggcagcgga
     2581 tggaacgcag gtctgggagt gcgacctcgg ttctcctgtg gcgcgtgcgc cggtggttct
     2641 ccccgcggag ggcggcgcac ctcccgttat cgtctgcggg gcggcgcggc agactctgtg
     2701 cggtatcacg cccgtcggaa gcatcgtatg gcggacaacg ccccgggctc atgtccaatc
     2761 gccccttggg tgcgcggatg ttgacgggga cgggcacctc gagatcatcc tcgggtgcgg
     2821 gcgggatcag aggacggtaa tcttcgacag agagggaaac gagaaggcat caatcccgta
     2881 ctctgccata tggacgaaca cggcagtacc gctcggcaac tcctcaccgg gaagggtgtc
     2941 tgtcctcgtg accggggatt ggaacggcga tatcgtcgcg gccaccccgc acatttgagg
     3001 accttgcggg gactggtctg ctgcgcgatg aacggaacgc agcgcgacca ggcgggcgct
     3061 gcggcccagc ggattgatcc ccggatcggt ctaccctatt ctctttagac aaagggcacg
     3121 ggcaacatat cccttctcca tcacacatcg gaggttctgg catgaaactc ggttgcagta
     3181 cgattctcta cggcgtccac agcacagagg acgctctcag cggcatcaag aaagcgggct
     3241 acgacggaat tgagctttgc gcgattcccg gaatgggctt tcatatcgag cccaacaaaa
     3301 ccgaaaagga atatcgggaa ctcaaaaaga aaatcgacga tcacggcctt attgtggaat
     3361 caataggggg aagcggcaac gacccgtacg atcccgatcc cgactccaaa ttccggtctc
     3421 tcatgcgagc agccgcaata atcggcgcgc cggcaattac cacgggatct ggcggaaaga
     3481 gcgataatca ggaagacatg aagcgggccg ccgaagtctt caacaagctt gcggagttcg
     3541 ggaaaacggt cggcaccaaa gtgagcatca aaccgcacgt cggaggagtc gtttacaaca
     3601 cgccttcgag cctcgaattc atgtcgctcg tagacacgaa atggatcggc ctcaacgtcg
     3661 acgcttccca cctctggcgc gcgccgaaat gggaagccgg tgaggagtct atcccaaaac
     3721 tcgcgaagta catattcact gcccggattc gtgataccct gaatcatgac cagccaatcg
     3781 gcccggtgga aacccaggtt cctggtggcg gcgccatgaa cctgaaagca atcatggacg
     3841 cgttcaaaaa ggtccccggg ttggatattg tcaccgtgga aatcgtagga acagcgcaat
     3901 gggagctgag cgcgatacag gacgttgttg aacgcaccta cgcggcgctc aaaaagctcg
     3961 tgtaaatgcg attgaagggc gatgggaggg tagccgggac gctgtcctcc cgtcctctgc
     4021 ggtacctcat cccgtggctt ctccgattgc ttctccatcc tgaaaaggcg cattgaatgg
     4081 taagaaccgt ggttctcggc ctcgacgaag ttggccgcga atacgcccgc gcatcaaaac
     4141 gggattcggc gtcaaacctt gtcggcgtgt acgacccgga ccgcgcgcgg gcacacagac
     4201 tcggcaagga actgggtgtc ccgatattcg accgtcctga agagctgctc gaagtggcga
     4261 aaccccaggt ctgcgtcctg tccgcattag agggaaaaga gacgctcgcc agcgatctcc
     4321 tctcacgggg aattcacgtg ttgtccacac tcccggtggc tcccgacgcg gaacaatgtg
     4381 agaagctcgc ggctcgagac agcgaaaaaa acggcattct tgcagccgac tttcatctgc
     4441 gcttcacacc ggccatggat aaggcgcgcg agtgggtgtc cgggggagag atcggctctc
     4501 cactgtttat caacatgaat ctctggcgag atgtcccgga cagacacggc gacaacccat
     4561 accatctgtt cgtctcgctc ggggcccacg gtgtggacgt catccgccag ttcgtcggag
     4621 gcgtcgagcg ggtgcagtgc ttcggcgtcc gcgacaaaca ggggatttgg acctcggccc
     4681 agatcaatat gatgtgctcc aacggtgcag tagggcatct gacgatcagt tacgagatgg
     4741 tgacacacca tcccatcgct cgttgcgaaa tcgccgggtc cagggcgcgc gtggtgatcg
     4801 agaacgtgta cgaggatgct acgctctacg tccacgaacg gcccgagaag ctggtcctta
     4861 ccaactccat attcggcggt atcccccagc tttcggagac ctggttccgg cgtctgcacg
     4921 agttccttac atctgttcag aagggaaatc tggctcgcga cacgaccgcc gttgatgcat
     4981 gtgccgccgc ccgggtaatg gaggctgcgt accacagcgc tgaacgcgga tcggttgagg
     5041 aggtggaatc ctgatgttga gaacctgcgt gataggcctc ggccccatcg gaaaccgaca
     5101 cgccgacatc catcaggctg atcctctcgc aactctcgtc ggtgtatgcg actggaatcc
     5161 cgaacgagca gaggccgccg gcaaacggct gggtgttcca ttcttcacgg acgcggcgaa
     5221 aatgctcgag agcctgcgcc cggacatggt gagcgtggca acgggtggcc acgaatacgg
     5281 aagcgaacac gcggctgcta cgatggtcgc ccttgaacac ggctgccatg tgctgtgcga
     5341 aaaaccgatt tccaacgaac tcgagcccgc gcgaaagatg gtcgcgctgg cacgcgaaaa
     5401 acggctctgt ttcgccgtga atcttaacca ccgtttcact cctgcgtgcc gccacgcgcg
     5461 gaaatggctg gccgaagggc gcatcggcat cccgcgtttc atgaacatgg cgatgtggat
     5521 cgggaacccc cgcgaaagca gcccgtggtt ccacttcaaa gcccttcacc cccatacgat
     5581 agacattatg cgctacttct gcggggaaat cgaagcagtg cacgcatttg ccatcaaggc
     5641 tccggggcgc agcatctggt ccaccgctca gttcaatttc cgcttcaagt ccggagcaac
     5701 gggacatctt accggcagtt acgacataat gcgagggcac ccgatggaac gcgccgaggt
     5761 gggcggcacg cgcgggcgga ttctgctgga ggacatgttc aaagaactga ccctgttccc
     5821 gcacgattcg agcgagaaga tcgtcgttac aaacccgatt atcggcggca tgggcgactt
     5881 cacggacacg tttatcaacc gcatccacaa cttcttcgtg gagctgaccg gaggcgttcc
     5941 acacgacagg atagatgggc aggcggcaga cgcgctcgcc gcacaggcag tgattgacgc
     6001 aaatatccga agcatgcgga ccgggaaggt cgagcgggtg gatcccgaaa gctggacggg
     6061 gaattacgac ctttcgtacg ggaaaccggg ccattccccg ggctgagaag aggaggcgtc
     6121 cgtgggtatc agtatctgta tggtcggcgt cggggccttc gcgcagagct tcatcccgct
     6181 tttcaaagcg cacccgcttg tggacgcgat ctccctctgc gatctcgacg agcgaaaact
     6241 ccgggaaaac tcccaacgct acggaatccc ccggacgtat gcctcgctcg atgaagcgtg
     6301 cgacagcgac gtggacgccg tcgccatcat cacccagaac tggatgcacg ccgaacaggc
     6361 tattcaggcg ttgaatgccg gcaaacacgt ctattccgcg gtgcccaccg gcataaccgt
     6421 ggacgaagtc gtcaggctgg taagaactgt cgaaaatacg ggcaaggtct acatgctcgg
     6481 ggaaacgagt tattactacc cggcagtaat ctattgtcgc gagcagttca ggaagggagc
     6541 attcggccgg gtagtgtacg gagagggaga atactatcac gactgggatc acgggctcta
     6601 cgatgtcatg aaatggcgcg gtggcgagcg ctggcgcgag tttgccggag gacctccgat
     6661 gcattacccc acgcactcta ccagccagat catttccgtn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnng ttgtcacctc gcaggacggc actcatctgc acgcgtcaaa
     6841 ggttcacgat gtggcccggc tgccgcggca gttcatcggg ttgccgaacg gccattgcgg
     6901 ttcgcaccag tttctcgtgg acgacttcgt ccgcgcctgc gccaaccgca ccgtaccgcc
     6961 aaacaacgtc tggcaggccg cgcgatacgc cctccccgga atcatcgcgc acgagtccgc
     7021 catgcaggga ggcgtcctgc tcgaagtgcc cgattgtgga gaccccccgc gcgagggata
     7081 gcaaagaacg cgaaaagccg caccagactc tctgatgcgg ctttttctct cgttgctcag
     7141 gtgcgattac tgcatcgcga acgaaatggt gaagtagttc acggaaccga gagatcctcc
     7201 catgtccgcg tacccgtaat cgaatgcacc ggccagaccg gcgtatccgt acttcacgcc
     7261 gaaaccggtg ttccacgtgc gcgcgtcgtt attgaacgtg tatccacccc gaacaaacac
     7321 catgtctctg aacgaatact ccccgcccag cgcaatcttc tccgggtggt cgggcggatg
     7381 gatgccgtcc accgcgatca ggagtttgtg ccccatttga cccagcgcgg ggatcggatc
     7441 gaacgcgttt cctgcaaccc cgatgtggaa cgtcatcggc gtggtgaacg cgtcgatcac
     7501 atgggtcgtt ttccgggaga agttccggat gctcatgctg acaaccgtgc tcccgaaccc
     7561 cgttctgtac aacgtccccg catcgaacac aaagttccgt gccgtaaact cggaaaggga
     7621 atcgccgacg gtaggaacgg cacccagatt gtcaggcggc ggaaccgcgt acgtggcgtt
     7681 tccgaagcgg taccacggca acccctggtt cacgtacttc ccggtcaggc ccagagaaaa
     7741 cttgtccgtg aactgcttcg ccagcgtcag accaagcagg aactcggacg gctcaagatt
     7801 cccgatgtca ctgtacgcgc cgttggtgga gcccttgtta atcaccgttc cgtgaattgt
     7861 gccgtaatcc ataccaacat aggaaattcc taccaccgcg aaccggtttc cgccaagttc
     7921 aatcggaact cctgccgccg cagaggcaat tttgatgtcg gcaatccagc tcacctgccc
     7981 cgccattgcg tcgacgcgac cggtgaggaa cccaagaccc gccgggttgt aaaagcaccc
     8041 ggtcgcgccc gtggaaacac cggtgtacgc atcccccatg gctgccgcgc gtgccaccgg
     8101 gccaattttg agaaaggtca tcccggattg accgagcttg tcgaactcac ccgccgccag
     8161 cacggggagc gaccacgcca gcacggcacc gagaaccagt attccgtatt tcgtgctttt
     8221 catggcatat ccctcccacg tctca
//
LOCUS       DAOSOO010000147         8198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8705_length_8198_cov_5.581849, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000147 DAOSOO010000000
VERSION     DAOSOO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8705_length_8198_cov_5.581849"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..2468
                     /locus_tag="PLT86_10270"
     CDS             204..2468
                     /locus_tag="PLT86_10270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT37036.1"
                     /translation="MPDAFIQSPDPVPFERLSNWQYHPGDMLPEEVWSTHDSWHEGGR
                     TLIWDDDVRIVWFREEWTVPRAWAGRALVLSIEVRNTFSLYVDEQAVTAPHLLSRSAG
                     PNRTFRLAIRGDRVRRPGMIMRAEIHSFPPEYAAWLDAKGALRAMAPGRGLLLEGWRV
                     HREEPGTDFADPLLDHSSWQARKLGEQWRERDSCYWYRMTVEVPEQIESFPVEGSRLH
                     LSASFNHSGSIWVDGEKKAEYARNFGNAVVTNHAKVGSRHVVALRVPTPWTGWVRDTY
                     LAPEGLLAARKAYDSLSGMVTFWDRFFQDRPEPQLIMAVCVVLADLAQKRLSGPALTT
                     AFTEASDELDRLVKTYSDDAPFLALPYLQLPSPGGIIVRAESPVARPAYLALRDCDGE
                     TILIPDEGSTRFHRFVVHGLQPDTPYSYTVGAGNVTTEINHFRTAPLTPRPITVFMWG
                     DSHYGPSVLEGLAERIEEVQPDLLITAGDMVGDGVNENEWRDYVFHPLRHVAAKYPLH
                     FPVGNHDHGSWRHRGHGDNPFLNERWEPSGTGWGNTPYGYSFDYAGVHFVFVDPLYGC
                     TGNETFADLNKGTAQYEWLERDLSASRGAQWTILLVHEPPFCETWEGGYYDGQPELRE
                     HLVPLMEKHGVDLCVSGHAHTYERGIPHPPFDPANGEGNTVAYLITGGGGSLLDNRKY
                     REWPQIDIPPHRVEQTENFLKNDIGEYYRYHYCVLRIEPQRLECTAYWIRLDGSVVDT
                     LDWFVLRKGIPARS"
     gene            2591..4444
                     /locus_tag="PLT86_10275"
     CDS             2591..4444
                     /locus_tag="PLT86_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT37037.1"
                     /translation="MSQHAPVPSKGAAIILLFLSGFCARMAAADTTYVMDPLVVTATR
                     VETERRNLPGSVTVIQRADLLATGATGVLDGIAASTPGVFVTQRGPAGFGLGSGAAGK
                     LTIRGIGGNPNSQVLVLVDGRPDFTGLFGHPLPDAYALSEVSRVEVVRGPSSAVYGTN
                     AMGGVINIITQRKRTVGNSLALRAAAGPWNTYDGEITALGKFASGLDYRVTSGYTTTD
                     GHRKKADFERAHFGAALGYSSGPWDVRGQLTTTPFETHDPGPVSPAITNDRWIDVTRS
                     SASVTGTYRRDALSSTLMVHRSWGTHKFYDGWHSNDYINGVIAQSSYSFVHGLVVTAG
                     ADAKQYGGDAVNDTTGYDYGTHRVTEIAPFATIQAPIGNLANLTASARAQHHDRYGWF
                     LAPEAGAAFHVLPRLTVRTNVSRGFRSPDLRALFLFPASRDTLKPEKTFNTEAGVSWR
                     ATDWLTSDITVFRTKASAIIENAPAPPFPPPFVNRGEFSSRGVEFETRWWISRIHGRL
                     SASLQDLGNQTAGSAETVVGGTVVLPAAQGRMALDCQWVHNLYGADRHQQKLNDYTVL
                     NATLAYPVWRGIEAQIVMRNLLDTKYETMPGYPMPGFHALVGVNAQIGSAE"
     gene            4441..4965
                     /locus_tag="PLT86_10280"
     CDS             4441..4965
                     /locus_tag="PLT86_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37038.1"
                     /translation="MNRSVATITRTGLVVALGLGFPWAIHMAGAGQIGKFLLPMFLPI
                     VGGAFFLPFGAAVFAGAATPFLSSILTGMPPLAPPIGPIMMVELAAMAGMVVLVYQKL
                     RANVWVTIASAVIVDRLILAFLAAGLGKMLKLPPAMITWATLVAGIPGMVLLFVVLPP
                     VVARLKAYSIREQT"
     gene            4962..5555
                     /locus_tag="PLT86_10285"
     CDS             4962..5555
                     /locus_tag="PLT86_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37039.1"
                     /translation="MNPARTSFFDEIAPQWDEKSGPEAIERVRAWCANLPLPSDGVVV
                     DLGCGTGISSQAIVEGTNRRVVAVDLSLEMLRNGRSKRDADSICWVCSNAMELPLADS
                     VADGVLALHVLPHLERRAVAFSEWRRVLKPGGSLFITHLMSRARVNEIHRSGPEAIRD
                     DILPPVRELAGELVFHGFCVLETEDSHERYLLHCARL"
     gene            complement(5596..7311)
                     /locus_tag="PLT86_10290"
     CDS             complement(5596..7311)
                     /locus_tag="PLT86_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT37040.1"
                     /translation="MKRRVLIIGGVAAGASAATKARRTDETAEIVMFERGDYVSFANC
                     GLPYYVAREIVERENLLVVTPEQFRIRHEIDVRLAHEVTRINPREKTIEVRDLQAGTS
                     RTERYDSLVLAPGASAIIPPIPMVNASNVFTMRTIPDMDAAHEYIERMHPQAATVVGA
                     GYIGVEMVEALHRRGLQVTLVEKLNQVLPPLDEDMAFMVADHIRAEGVDLHLGDGVRS
                     FRGNDDRVVEVELESGAVIHTDMVIMSIGVRPDTKLARDAGLQLGVSGAIAVDSRMQT
                     SDPHIWAAGDAVESVHRVTGKPCWVALAGPASKQGRVAGANAVGGTMEAPPVLGTSIV
                     RFNEIVAAVTGLNERSARTAGYDVESSVIHANQHAGYYPWAELLTIKTVYERQTGRLL
                     GAQVIGNEGVDKRIDVFATALTANMRVADLTELDLAYSPPFGSARDPVNIAGFVSQNQ
                     LSDAVSPISSTDLHALLGQSPEVQLVDVRTPAEFREKHIRGARLIPVDELRKRLGEID
                     KTRPVVVYCRVGLRGYLASRILAQNGYSVRNLSGGIRIWRFETEADISDAEAKSKYGN
                     DVSRA"
     gene            complement(7385..7768)
                     /gene="trxA"
                     /locus_tag="PLT86_10295"
     CDS             complement(7385..7768)
                     /gene="trxA"
                     /locus_tag="PLT86_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT37041.1"
                     /translation="MAHENQVQEAVHPLEVTDQTWQAEVMEADLPVFVDFWAPWCGPC
                     RMVAPVVEQLATEYAGKVKFVKLDTDANPVISAQYGIRSIPTLAVFHKGRPIHAMMGA
                     GPLGHMRRFVEDSLAKVPGHIIVAR"
BASE COUNT         1599 a         2480 c         2426 g         1693 t
ORIGIN      
        1 gtgggcaagg tgggtaaccg gggtggtttt tgtcgggatc ggaatctact tcacactggt
       61 gtacacgctg gagttgtact gaattccggc accggaacac accggttccg gtccccctgc
      121 ccagttgcct ttcaggcttc gcaagtctac cctttttcag tctgttactt tgtttcgtgt
      181 accgcgacga cgaaggatat tacatgcccg acgcgttcat acaatccccc gaccctgttc
      241 cgttcgagcg cctttccaac tggcaatacc atcctggcga catgctcccg gaagaagtat
      301 ggagcacaca tgatagctgg cacgaaggcg gccgcaccct catctgggat gacgacgtgc
      361 gtatcgtgtg gttccgcgag gagtggacgg ttccacgcgc atgggcgggc agggcgcttg
      421 tcctttccat cgaagtcagg aacacctttt cgttgtacgt ggacgagcag gcagttactg
      481 ctccgcatct gctcagccgc agcgcaggtc cgaacagaac attccgcctc gcaataaggg
      541 gagaccgcgt tcgccggccc ggaatgatca tgcgagcgga aatccactcc ttcccgcccg
      601 agtacgccgc gtggctggat gcgaaagggg cgttacgggc tatggcgccc gggcgcggcc
      661 ttctgctgga aggctggcgc gttcaccgtg aggaaccggg aaccgatttc gccgatccgc
      721 tcctggacca ttcatcctgg caagctcgaa aactcggtga gcaatggcgc gagcgcgata
      781 gctgctattg gtaccgaatg accgttgagg taccggaaca gatcgagagc ttccccgtag
      841 aaggatcccg gctgcacctc tccgcatcgt tcaatcattc gggcagcatc tgggtggatg
      901 gcgagaaaaa ggccgaatac gcaagaaact tcggcaatgc cgtagtcacg aaccatgcga
      961 aagtcggctc acgccacgtg gttgcgttgc gcgtgccaac gccatggacc gggtgggtac
     1021 gggataccta tctcgccccg gaaggtcttc ttgccgcccg caaggcgtac gacagccttt
     1081 ccggaatggt gaccttctgg gatcgtttct tccaggatcg tccggagcct cagctaatca
     1141 tggcggtgtg cgttgttctg gcggacctgg cacaaaaacg gctctcggga ccggcgctta
     1201 ctaccgcttt caccgaagcg tcggacgaac tggaccgtct ggtaaagacg tactcggacg
     1261 acgcgccgtt ccttgcgctg ccctatcttc agttgccctc gccgggaggc atcatagttc
     1321 gcgcagagtc gcctgtggcg cggccggcat accttgcgct gcgcgattgc gacggggaaa
     1381 caatcctgat cccggacgaa gggtccaccc ggttccaccg gtttgtggta cacggcctcc
     1441 agcccgatac gccgtattcc tacactgtcg gagccggcaa tgtaacaact gaaatcaatc
     1501 acttcaggac cgcgccgctg acaccgcgcc cgattaccgt tttcatgtgg ggggacagcc
     1561 actacgggcc cagcgtgctg gaaggattgg ccgagcgcat cgaagaagtg caacctgatt
     1621 tgctgatcac cgccggcgat atggtgggtg atggcgtgaa cgagaacgag tggagggatt
     1681 acgtcttcca cccgctacgc cacgttgcag cgaaatatcc attgcatttc cctgtcggca
     1741 accacgatca tggcagttgg cgacatcgcg ggcacggcga caaccctttt ctgaacgagc
     1801 ggtgggagcc atcgggaact ggctggggca acacacccta cggatacagc ttcgactatg
     1861 cgggcgtgca tttcgtcttt gtggaccccc tgtatggctg cacgggaaac gaaaccttcg
     1921 cggacctcaa caaaggaacc gcccagtatg aatggctcga gcgggatttg agcgcttcgc
     1981 gcggcgcaca gtggacaata ctgctagtac acgaacctcc cttctgcgaa acatgggaag
     2041 gcgggtacta cgacggtcaa cccgagctcc gtgagcatct cgtcccgctc atggagaaac
     2101 acggggtgga cctctgtgtt tccggccatg cgcataccta cgaacgcgga atcccgcacc
     2161 cgcctttcga cccggcaaat ggcgagggaa acacggtcgc gtacctgatc accgggggcg
     2221 gtggttccct cctcgacaat cggaagtacc gcgagtggcc acagattgac atcccgcctc
     2281 accgggtcga acagacggaa aacttcctga aaaacgacat cggtgaatac taccggtacc
     2341 attactgcgt gttgcgcata gaaccgcagc gactggaatg cacggcgtac tggattcggc
     2401 ttgatggctc agtggttgac acgctggact ggttcgtgct gaggaagggt attcccgcga
     2461 ggagttgacc gagcgttcgg gtcccaggac cgggatctgt caccggaagg gttgtggacg
     2521 tctgtcggtg ttactatttt caaattcgtc acacgattct gacaaatgct gacgatctcg
     2581 ggaggaaccc atgtctcaac acgctccggt cccctctaag ggcgccgcaa tcattctgct
     2641 tttcctgagc ggcttctgtg cgcgaatggc agcggccgac acgacatacg tcatggatcc
     2701 gctcgtagtc actgccaccc gtgtggaaac cgaacgacgc aacttgccgg gcagcgtcac
     2761 cgtcatccaa cgggcggatc ttctggcgac cggagccacc ggcgttcttg acggcatcgc
     2821 cgccagcacc ccgggagttt tcgtcaccca gcggggtccg gccggtttcg gactcggatc
     2881 aggggcggca ggcaagctta caattcgtgg aattggtggc aaccctaaca gccaggtact
     2941 ggttctggtg gacgggcggc ctgatttcac cggcctcttc gggcatcccc tacccgacgc
     3001 gtacgcgttg agcgaagtca gccgcgtgga agtagtgcgc ggaccgtcgt cagcagtgta
     3061 cggcacgaac gccatgggcg gggtcatcaa tatcatcacg caacggaaac gcactgtggg
     3121 caactcgctt gcactgcgcg cggcagcggg tccctggaac acgtatgacg gcgagatcac
     3181 cgcgctgggc aagttcgcca gcgggttgga ttaccgtgtc accagcggct acaccaccac
     3241 ggatggacac agaaagaagg ccgattttga gcgcgcacat ttcggcgctg cgctcggtta
     3301 cagcagtgga ccgtgggatg ttcgagggca gcttacgacg acgcccttcg aaacgcacga
     3361 cccggggcca gtttccccgg cgatcaccaa tgaccgatgg attgacgtga cacgcagcag
     3421 tgcttccgtc acgggtacct atcgaagaga cgccttatcc tccacgttga tggtgcaccg
     3481 cagttgggga acgcacaagt tctatgacgg ctggcacagc aacgactata tcaatggagt
     3541 aatagcgcag agcagctact cattcgtgca cgggctcgtc gtgacggcgg gtgcggacgc
     3601 gaagcagtac ggcggcgacg cggtgaacga cacaacaggt tacgactatg gcacacaccg
     3661 cgtgaccgaa atagctccat tcgctacgat tcaggcaccg attggcaatc ttgccaacct
     3721 tactgcctcc gcacgcgcac agcaccacga tcggtacggc tggttcctcg ccccggaggc
     3781 cggcgccgcg ttccatgttc tgccacggct cacagttcga acgaacgtgt cccggggctt
     3841 ccggagtccg gacttgagag cgctgttcct gttccccgct tcgcgtgaca cgttgaaacc
     3901 ggagaagacg ttcaatacgg aagcaggtgt cagctggcgc gccaccgact ggctgacttc
     3961 cgatatcacg gtgtttcgca ccaaagcgag cgcgatcatc gaaaacgccc ccgcgccacc
     4021 gtttcctcca ccattcgtga accgcggtga attcagttcg aggggcgtgg aattcgagac
     4081 gcgatggtgg atatcgcgca tccatggtcg tctttcggcc tcgttgcagg atcttggcaa
     4141 tcagacggcg gggagtgcgg aaacggttgt ggggggtacg gtcgtgctac cggccgcaca
     4201 ggggagaatg gctctggatt gccagtgggt gcacaacctg tacggtgccg accgtcacca
     4261 gcagaaactc aacgattaca ccgttctcaa cgcaacgctg gcgtacccgg tctggcgagg
     4321 gattgaagcc cagatagtga tgcgcaacct tctggacacg aagtacgaaa ccatgccggg
     4381 gtatccgatg cccggcttcc atgcacttgt gggcgtgaac gcgcagatcg gctccgcaga
     4441 atgaaccgtt ccgtagccac aatcacgcgc acagggctcg tcgttgcact tggtcttggc
     4501 tttccctggg cgattcacat ggcaggcgcg ggccagatcg gcaagttctt gctgcccatg
     4561 ttcctgccga ttgtgggggg agcgttcttt ctccccttcg gcgcggcggt gttcgccggt
     4621 gccgccacgc ctttcctatc ttcgattctc accggtatgc cacccctcgc gccgcccatc
     4681 ggaccgatca tgatggtcga acttgccgcc atggcgggga tggtagttct ggtgtatcaa
     4741 aaactgcggg ccaacgtatg ggtaaccatc gcctccgctg ttattgttga tcgcctcatt
     4801 ctggcctttc ttgccgccgg gctcgggaag atgctcaagt tgccacccgc gatgataacc
     4861 tgggctactc tggtggcggg tattcccgga atggtccttc tcttcgtcgt tctgccacca
     4921 gtggtggcgc gtctcaaagc ctactccatc cgggaacaga cgtgaatccg gctcgcacaa
     4981 gcttttttga cgaaatcgca ccccagtggg acgagaagag cggccctgag gctatcgaac
     5041 gtgtccgggc atggtgcgcg aacttgcccc tgccctcgga tggcgttgtt gtggatctcg
     5101 ggtgcgggac agggatctcg tcgcaggcaa tcgttgaggg gacgaaccga cgcgtagttg
     5161 cggtcgacct ctcgctcgag atgctccgga acgggcggtc aaaacgggat gcagattcga
     5221 tttgctgggt atgcagcaac gcgatggaat tgcctctggc agattcagtt gcagacgggg
     5281 tgctcgcgct gcacgtgttg cctcacctgg aacgccgagc cgtcgcgttt tccgagtggc
     5341 ggcgtgtgtt gaagcccggc ggatccctct tcattacgca cctgatgtcc cgggcccgcg
     5401 tgaacgagat ccaccgatcc gggcccgaag ccatacgcga cgacatcctt ccccccgtgc
     5461 gggagctggc tggggagctg gtctttcacg ggttctgcgt cctcgaaacg gaggactcgc
     5521 acgaacggta cctgcttcac tgcgcacggc tctgaagagc ggtcgagagc ctcccgcgga
     5581 ccggttgcgc ggcgctcagg cccgggaaac gtcgttcccg tatttcgact tcgcctcagc
     5641 atccgaaatg tcagcctctg tttcgaaccg ccagattcga atgcctcccg ataggtttct
     5701 caccgagtac ccattttgcg caaggattcg actcgcaagg tagccccgga ggcccacccg
     5761 gcagtacacg accaccggcc tggttttgtc aatttcgcca aggcgttttc ggagttcgtc
     5821 aaccggaatg aggcgcgccc cgcgaatgtg tttctctcga aattcggcgg gagtgcgcac
     5881 gtcaacgagc tgaacctccg gcgattggcc gagaagggcg tgcaaatccg tactggaaat
     5941 cgggctcacg gcatccgata gttggttctg tgaaacgaac cccgcgatat tcaccggatc
     6001 ccgcgcagag ccgaagggtg gcgagtacgc gagatccagt tccgtcaggt cggcaactct
     6061 catgttcgcc gtaagggcag tggcgaacac atcgatgcgc ttgtccaccc cttcgttgcc
     6121 gatcacttgc gcgccaagca gacgccctgt ctgtctctcg tagacggtct ttatcgtgag
     6181 gagctccgcc caggggtaat acccggcgtg ctggttggca tgtattacag agctttccac
     6241 atcgtaaccc gcagtgcggg cggacctttc gttcaatcct gtcaccgccg ccacgatctc
     6301 gttgaaccgc acgattgacg ttccgagaac cggcggggct tccattgttc cgcccactgc
     6361 gttggccccg gctacccgcc cctgcttgct cgccggaccg gcgagcgcga cccagcacgg
     6421 ttttcctgta acgcggtgca cactctcaac cgcgtctccc gccgcccaga tgtgcggatc
     6481 gctcgtctgc atgcgggaat ccaccgcgat cgcgccactc accccgagct gcaatcccgc
     6541 gtcgcgcgcg agcttcgtat ccgggcgaac tcctatggac atgataacca tgtcggtgtg
     6601 aatgacggct ccactctcga gttccacttc gacgactctg tcgtcgtttc cccggaagga
     6661 gcgcacgccg tcgccaagat gaagatcgac tccctcagcg cggatatggt cagcaaccat
     6721 gaacgccatg tcctcatcca gaggaggaag cacctggttc agcttttcca cgagggtaac
     6781 ctgaagcccc cttcggtgca gagcttcgac catctccaca ccgatatacc ccgctcccac
     6841 gacggtggcg gcctgcggat gcatgcgctc gatgtactcg tgcgcggcat ccatgtcggg
     6901 gatagtccgc atcgtgaaca cgttcgacgc attgaccatc ggaatcggcg ggattatcgc
     6961 gcttgcgccg ggtgccagga cgagggaatc ataacgctca gtgcgtgacg tgcctgcctg
     7021 caggtcgcga acctcgatcg ttttctcccg ggggttgatg cgcgttacct catgggcaag
     7081 tcgcacatca atttcgtgcc gtatcctgaa ctgctcaggc gtcacgacga gaaggttctc
     7141 gcgctccacg atttcgcgcg cgacatagta tggcagcccg cagttcgcaa acgaaacgta
     7201 atcgccccgc tcaaacatta cgatttcagc ggtttcgtct gtgcggcgcg ctttcgtcgc
     7261 cgcgctcgca cccgcggcaa ctccaccaat gatcaatacg cggcgtttca tgtgcttctc
     7321 cagagatgag ccgcggcggt gaggcgagga atcctcactg ccgcgactcg gattcgggcg
     7381 ggaatcaacg ggcgacgata atgtgaccgg gcaccttcgc aaggctgtcc tcaacgaacc
     7441 tgcgcatatg cccgagaggc cccgcaccca tcatggcgtg tatagggcgc cctttgtgaa
     7501 acacggcgag cgtcgggatg ctacgtatcc cgtactgcgc ggagatgaca ggattcgcgt
     7561 ccgtgtcgag cttgacgaat tttaccttgc ccgcgtattc ggtcgcaagt tgctccacga
     7621 ccggcgccac catgcggcag ggaccgcacc acggtgccca gaaatcaacg aataccggaa
     7681 gatcagcttc catcacctcg gcctgccagg tctggtcggt tacttccagt gggtgtactg
     7741 cttcttgaac ctgattttcg tgtgccattg ttgcctccag agttggattg tgatttctga
     7801 cgtgatcatt tgcaactctg gtgccaacgg ctacggatga ctggcaatgc ttcctcaagc
     7861 atctttaata tttgacactt gaatgacatg agattcgttc gcttcaaggc tcgcgaacgc
     7921 gcgtctggtg tttcacgaaa catcctgatg aaacaatata cgcctggtga aacatattgc
     7981 aacacttccg ttgtggatca gcggcccgac gtattccttg gcgaacgagg ccgccatgac
     8041 gtacacttgt acttgagccg gcggctgttc ttagcaacgc cggtatttct gtgtcttgaa
     8101 cgcaaccaac ggcacccaag gagtgtttca tgagtcgcgt gttgaacgtc gcggtggtcg
     8161 gtgcaacagg cgttgccggc cagcaggcac ttgtttcg
//
LOCUS       DAOSOO010000148         8165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8757_length_8165_cov_6.220715, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000148 DAOSOO010000000
VERSION     DAOSOO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_8165_cov_6.220715"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLT86_10300"
     CDS             complement(<1..1025)
                     /locus_tag="PLT86_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37042.1"
                     /translation="MYSPRALAFGLLLLAFPLLANATPVTGNLSTQTWTKTRSPYRVT
                     GKVTVLSGETLTIEPGVDVLFDADVQFHVRGRILALGAPLDSVRFLKGSAARWGGMRI
                     IKADSCVFHYVRFSDSHRRDASDKWEDISGGAVFVDSSVAVTMANTVISRNVADGYGG
                     GICIAHTKVTLTDCTIADNSAAEYGGGLSNNYATASLTMTNCLVVGNTAVKYGGAIEN
                     WGAKTEILGCVFFGNTATASGGTSDGSMLVNWGTSPTVTIRNSIVWGNAPVNAIRVDA
                     GTVTATYSDIQRSSGTFAGTGNINLNPQFVNTGVRNFRLLPASPCINTGDPGSPLDAD
                     GSRADMG"
     gene            complement(1203..2672)
                     /locus_tag="PLT86_10305"
     CDS             complement(1203..2672)
                     /locus_tag="PLT86_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOT37043.1"
                     /translation="MPADKKYPNIVIMYADDLGFGDVGCYGATEIPTPNVDRLAADGI
                     RFTNGYATAATCTPSRYSLLTGAYPWRNPRAAILTGDAPIIIEPGSPTLPGMLQRAGY
                     RTGVVGKWHLGLGQGDLDWNRDISATPNDVGFDHSFIMPATNDRVPCVYLENRRVVNL
                     DPDDPIEVTYADKKVFPGELTGREHPELLRVTHSHGHDGTIVNGVGRIGHMRGGRSAL
                     WTDETMAETFAKKAVEFIAANREQQFFLYYALHQPHVPRLPNPRFKGATKLGARGDVI
                     VEMDWCVGEMLNALDRLGIRENTIVIFSSDNGPVLDDGYRDGAEELRDGHRPAGPLRG
                     GKYSLFDGGTRIPFILSWPGSVQPGESAALVSHVDFHASFAALTGQQLGHEEAPDSLN
                     VLDALLGRSGTGREELVVEGTRSKTVYRRGNWTYIPPYQGPQVNVNTNTELGNSLDPQ
                     LYDLSQDIGQITNLAAAFPARAAEMQARLDSIRQGTCSR"
     gene            complement(2710..4425)
                     /locus_tag="PLT86_10310"
     CDS             complement(2710..4425)
                     /locus_tag="PLT86_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37044.1"
                     /translation="MLRYSVPGDEFQLDFRLVEAGEPVTFNLRGVTSRDRFEIFPRYL
                     ETCDPERARLSPAPLAWLDELGPVELPAGEVIRYTPEVPGNYLARWTSRQYGVEYRYF
                     AAVDQSYVIYRPAVWNWPTPFPASGGADIHNGGLPLDWCISSDSTDEPYVPRLRDEQQ
                     RYGGGVVYGMNTASADGPIDGAVRALRDTVANLRQHGLDVGRVANLWYGGGLSHDRVA
                     LARAVGFDVIDGYVPRAASCGLGAPYFPFYIGSVDYRFPGQHGPTDTLAFVFDFVGSW
                     HFHGPIGFHRPTANGSWEWARHYIDLAAREASLNAKNSGAHNVITTLVNFESPIAWGN
                     PAYQVVWDEGRGRDFFDNYMHLLAFEHTRRWPIVFARAADYADYFRAHYTGMPRRIVS
                     SITHDIEYDRYWTDEWHEKGLTPAGYLPVNQPLRAFREARAMPQYNMPVSHEFMNYND
                     NRRTCRFEYACPKPLHYYDLTGAEPWPQQPAETDIPDPDITISIVAHRDSYEITYAFS
                     AGASFADYLVAVWDIPRECRNRRMETNAKEFIWIENTDGNCRGIVRFDLEPECTISVR
                     WHGNS"
     gene            complement(4477..5124)
                     /locus_tag="PLT86_10315"
     CDS             complement(4477..5124)
                     /locus_tag="PLT86_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37045.1"
                     /translation="MNQNVAELNRDCEDYSKWWRAVDQELYHYFAEHSDHGNLRSVYA
                     KVAMVDRLYTAGLERARKGTDEIDRAQKVAELLFARGEELDCGIRMISGVPCLTRDAL
                     ADIAALHGWIAETCLKEAGVGAPVSFVSKYLHFHVPVVPIYDQQACASLARYIRGRFG
                     RYVATPERLPKLDGQMPAYRWYCARFAELWEEAQESRPETTVKELDYHLWVLGKT"
     gene            complement(5491..6282)
                     /locus_tag="PLT86_10320"
     CDS             complement(5491..6282)
                     /locus_tag="PLT86_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014978934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT37046.1"
                     /translation="MADISVIDGNIFTSRAQTLVNTVNCVGVMGAGIALECRLRYPEM
                     HDQYVSLCRRRMIEIGSLWIYKAPDRWILNFPTKRHWKEPSKEEYLHAGLRKFMQTYQ
                     EKQIESIAFPLLGAQHGGLRRDRSQELMESYLSECRIPIEIYRYDPMAPDDLYDRFCE
                     LLRDMTPEEMKVETGLRSDFVKRLLDAVHDPQVRQLNRLASLDGIGIGTLEKVFAFAR
                     GRILGRSQPVQQALEFGRVAEPTATFDHKNCSSYREGRKGKHIVG"
     gene            complement(6295..6867)
                     /locus_tag="PLT86_10325"
     CDS             complement(6295..6867)
                     /locus_tag="PLT86_10325"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOT37047.1"
                     /translation="MPVVLWRHFYHITHRDNLRNIFRYGILSWHCAHQRGLTASDISD
                     PQVQERRNRIEPVFRRSIHEYAPLYFNPKNPMLFRRRELQEELVILEVSPDVLRYNQH
                     IFTDGNAAAATTTFSTNREIIAASEDALRAEWWTALPDGKRRRCAEVLIFPSVTPRFI
                     TNAICANQGSASAINWTCPVSVIVDTSFFF"
     gene            complement(6939..>8165)
                     /locus_tag="PLT86_10330"
     CDS             complement(6939..>8165)
                     /locus_tag="PLT86_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37048.1"
                     /translation="VGVGRGQSFASSATDLVGMKLVALCDLWEERLLEVGKRYHVATY
                     TDYDRFLEHDMDAVILANYFHEHAPFAIKALAAGKHVMSECICNATLGEGVALCRAVE
                     KSGLIYMLAENYPYTAFNMEMRRVYQTGEIGRVTYAEGEYNHPMAPEDRLRIAPGMNH
                     WRNWIPSTYYCTHALAPLVFITDTMPVMVNGLSIACREPDTKTVRRGDPGSVILCRMD
                     NGSVFRIFGLTLPGHSNWYRVHGTKGAMEITRGPGYYGPGQVRVWHEEWDMKPGELRD
                     RIYLPDWPVHADLARKAGHGGGDFWTNFEFANAIRSGTQPFLDVYRGVAMSSVGILAW
                     KSALKDGMPLEVPDFRNETKRTKYAKDNASPFPSHAGKKGYTRLPASIAGDLKPTKEG
                     LALAKRVWKEIGYKGD"
BASE COUNT         1526 a         2486 c         2409 g         1744 t
ORIGIN      
        1 atccccatgt cggcgcgcga tccatctgcg tcaagcggag agccgggatc acctgtgttg
       61 atgcagggcg atgccggcaa aaggcggaaa ttgcgcacgc cggtgtttac aaactgggga
      121 ttgagattga tattgccggt tccggcgaac gttccggacg accgctggat gtccgaatac
      181 gttgcggtga cggtgccggc atcaactcgg atggcattga cgggggcgtt tccccagacg
      241 attgagttgc gtatcgtcac cgtggggctg gttccccagt tgacaagcat gcttccgtcc
      301 gaggtcccac ctgaagcggt agcggtgttg ccgaaaaaca cacagccaag gatttcggtc
      361 tttgcccccc agttttctat tgcgccgccg tatttgacgg ccgtattgcc caccacgaga
      421 cagttcgtca tggtcagcga cgcggtggca taattgttcg aaaggcctcc gccgtattcc
      481 gcggctgagt tgtccgcgat ggtgcaatcg gtcagcgtca ctttcgtgtg ggcgatacag
      541 atacctccgc catacccatc cgcaacattc ctcgagatca cagtgttcgc catggttacc
      601 gcaacagacg agtcgacaaa caccgcgccg ccgctgatgt cctcccattt gtcgctggca
      661 tcgcgccgat gcgagtcact gaaacggacg taatggaaca cgcatgaatc ggccttgatg
      721 atgcgcatcc cgccccatct ggccgccgag cccttcagaa aacggacgct gtccagcggc
      781 gcgccgagcg ccagaatacg tccccgcacg tggaattgca catcggcatc gaacaggaca
      841 tccacgccgg gctcaatggt gagtgtctca ccggaaagca ccgtcacttt gccggtgacg
      901 cggtaggggc ttctagtttt cgtccatgtc tgcgtggaaa ggttcccggt gaccggagtg
      961 gcgttcgcga ggaggggaaa ggccagcagc aacagaccga aagcgagggc acgcggtgaa
     1021 tacatggcga ctcctctacg tttcccacct cgcgtggtta tgggatcctc gcgtgaggca
     1081 ggccggtgaa gtacccccca gtacaaccgt caaatatata tcgttcacaa aaaaattgga
     1141 aacgggaata tcgggaccgt tttcggaact cgcctccgcc ggccgcaatg gcgtccgtcg
     1201 ggtcagcgcg agcatgttcc ctgccggatc gagtccagcc ttgcctgcat ctctgccgcc
     1261 cgcgcgggaa acgccgccgc gaggttggtg atctgcccga tgtcctgcga aagatcatag
     1321 agctgcgggt ccagcgagtt gccaagttca gtattggtgt tgacgttgac ttgcggcccc
     1381 tggtagggag ggatatatgt ccagttgccg cgccggtaca ccgtcttgct gcgagttccc
     1441 tccacgacca gctcctcgcg tcctgtgccg ctcctgccaa gcagagcatc cagcacgttg
     1501 aggctgtccg gtgcctcttc gtggcccagt tgctgaccag tcagcgcggc gaacgacgcg
     1561 tggaagtcaa catggctgac gagcgcggcg gattcgccgg gttgtacact gcccggccag
     1621 ctcagaatga atgggatgcg cgtgccgcca tcgaacaggc tgtatttccc gccccggagc
     1681 ggaccggccg ggcgatggcc gtcccgcagt tcctccgcgc cgtcccggta gccgtcatcc
     1741 agcacaggac cgttgtcgct tgaaaaaatg acgatggtgt tctcccgtat gcccagccga
     1801 tccagcgcgt tgagcatttc gccgacgcac cagtccatct cgactatgac gtctccccgg
     1861 gcgccgagct tcgtcgcgcc tttgaagcgc gggttgggca ggcgcggcac atgaggctgg
     1921 tggagggcat agtagaggaa gaactgctgt tcacgatttg cggcgatgaa ctccaccgcc
     1981 ttcttcgcga acgtttcggc catggtttcg tcggtccaga gagcactcct gcccccgcgc
     2041 atgtgcccga tgcggcccac cccattcacg atggttccgt cgtgcccgtg gctgtgggtt
     2101 accctcagaa gctccgggtg ttcccgtcct gtcagttcgc caggaaacac cttcttgtcg
     2161 gcatacgtca cctcgatcgg gtcatcagga tccagattca ccacccgccg gttttccaga
     2221 tacacgcacg gcacgcggtc gttggtggcc ggcatgatga aggagtggtc gaacccgacg
     2281 tcgttcggcg tggcgctgat gtcccggttc caatcgagat cgccctgccc gagaccgagg
     2341 tgccatttgc cgacgacgcc agtgcggtac cccgcccgct gcagcatgcc gggcagggtc
     2401 ggggatcccg gttcaatgat gattggtgcg tcgccggtga ggattgctgc gcgggggttt
     2461 ctccacgggt aggcgcccgt cagcaggctg tagcgtgacg gtgtgcacgt tgccgccgtg
     2521 gcgtagccat tcgtgaagcg gatgccgtcc gcagccagcc ggtccacgtt cggggtgggg
     2581 atttcggtcg ccccgtagca gccgacgtcg ccgaaaccga ggtcatcagc gtacatgatg
     2641 acgatgttgg ggtatttctt gtcggcaggc attccagaat tccctttcgc ttctaccgca
     2701 tctttgccgc tatgagtttc catgccatcg cacagagatc gtgcactcag gttcgaggtc
     2761 gaagcgcact attccccggc agttgccgtc cgtgttctcg atccagatga actctttggc
     2821 gttcgtctcc atgcgccgat tgcggcactc gcgcgggatg tcccatactg cgaccaggta
     2881 atcggcgaaa ctcgcccctg cggaaaaagc gtacgtgatt tcgtagctat cgcggtgcgc
     2941 cacgatcgag atggtgatat ccgggtcggg gatatcggtt tccgccggct gttgcggcca
     3001 gggttccgca cccgtcaggt cgtaatagtg gagcggcttc gggcaggcgt attcgaaccg
     3061 gcacgttcgc cggttgtcgt tgtagttcat gaactcgtgg gaaaccggca tgttgtactg
     3121 cggcatggcc cgggcttcgc ggaatgcgcg aagcggctgg ttcacgggca ggtaacccgc
     3181 gggcgtcaaa cccttctcgt gccattcgtc ggtccagtac cggtcgtatt cgatgtcgtg
     3241 cgtgatcgaa ctcacgatac ggcgcggcat cccggtgtag tgggcgcgga agtaatccgc
     3301 gtagtcggcg gcgcgggcga acacgatggg ccatcgccgc gtgtgttcaa atgccagcag
     3361 gtgcatgtaa ttgtcgaaga aatcccggcc ccgcccctcg tcccagacga cctgatatgc
     3421 cgggtttccc catgcgatcg ggctttcgaa gttcaccagc gtcgtgatga cattgtgagc
     3481 gccgctgttt ttcgcgttga gagaggcctc gcgggctgcc agatcgatgt aatgccgcgc
     3541 ccactcccag ctcccgttgg cggtcggacg gtgaaacccg atcgggccgt ggaaatgcca
     3601 gcttccgaca aagtcgaata cgaacgcgag cgtgtcggtc gggccgtgtt gaccgggaaa
     3661 acggtaatct acggaaccga tgtagaacgg gaaatacggt gccccaagcc cacaactggc
     3721 cgcgcggggc acatacccgt caatcacgtc gaatccgacg gcgcgcgcca gggcaaccct
     3781 gtcgtgtgaa agcccgcccc cgtaccacag attggccacc cgcccgacat cgaggccgtg
     3841 ctggcgaagg ttcgcaacgg tgtccctgag cgcacgaacc gcgccatcaa tcggtccgtc
     3901 cgcggacgcg gtgttcatgc cgtagacaac gcccccgccg taacgctgct gttcgtctcg
     3961 caaccggggc acatacggtt catcggtgct atctgaactg atgcaccagt cgaggggcag
     4021 gccgccgttg tgaatgtccg ccccgcccga cgcggggaac ggcgttggcc agttccagac
     4081 tgcggggcgg tagatcacgt agctctggtc caccgcggcg aaatagcggt attcgacacc
     4141 gtattgccgg cttgtccatc gagcgaggta gttgcccggc acttccgggg tgtagcggat
     4201 aacctcaccg gcgggcaatt caaccggtcc aagttcgtcg agccaggcca gcggcgcggg
     4261 ggagaggcgg gcgcgctccg ggtcgcaggt ttccagatac cgcgggaaga tctcgaaccg
     4321 gtcgcgagag gtcaccccgc gcaagttgaa cgtgaccggt tccccagctt ccaccagtcg
     4381 gaagtcgagc tggaattcgt caccggggac actatatcgg agcaactttg tcaccttcgg
     4441 tgtcgacgca aagcagtcag gctgcgcctt cgcaagttag gttttgccca acacccacaa
     4501 atggtagtcc aactccttca ctgtcgtttc ggggcgagat tcctgtgcct cctcccacaa
     4561 ttcggcgaaa cgagcgcaat accaccggta cgcaggcatc tgcccgtcca gtttggggag
     4621 gcgctcagga gtagcgacat atctcccgaa cctgcctcgg atatacctgg caaggcttgc
     4681 gcacgcctgc tggtcgtaga tggggaccac gggaacgtgg aagtgcaggt atttcgagac
     4741 gaacgagacg ggggcaccga ctcctgcttc tttgagacag gtttccgcaa tccatccatg
     4801 caacgccgct atgtccgcaa gagcgtctct cgtcagacac ggcaccccgc tgatcatccg
     4861 aattccgcag tccaactcct ccccgcgagc gaaaagcaac tcggcgacct tctgcgcccg
     4921 gtctatttcg tctgttccct tgcgtgcacg ttccaagcca gccgtgtaga gacggtcgac
     4981 catcgcaact ttggcgtaca cggaacgaag attcccgtgg tcagagtgct cggcgaagta
     5041 atggtaaagc tcttggtcta ccgcccgcca ccatttggaa taatcctcac agtctctgtt
     5101 gagttcagcc acgttctggt tcatcgcggc tccctttccc cacacaggta atgctcaact
     5161 ccaaacacct ccgctgctgg cagtgattcg cgcggtttgc ccacggcttt ttccggtcct
     5221 cactccccct tgtacccgat ctctttccac acgcgtttcg ccagcgcgag tccttctttc
     5281 gtcggcctca ggtcgccggc gatgcttggg ggcaggtgct tgcggccaca tttcgcgtgc
     5341 aacggatact tggaggcgtt gtcagtcgct tgtcgtttat ccccgactcg atcgctcccg
     5401 ggtcactggg taatcttgct ccccggggcc atcgcccacc actcgggccg aaaacccaca
     5461 cctgcgacgc cgattgcgat tgaaacccga tcagcctacg atatgctttc ctttcctccc
     5521 ctcgcggtag ctgctgcaat ttttgtggtc gaatgtagcc gttggttccg cgacccttcc
     5581 gaattcgagg gcctgttgta cgggttgact gcgaccgaga atccgaccac gtgcaaaggc
     5641 aaacaccttc tccaatgtgc cgattccgat gccgtcgagt gatgcgagac ggttgagttg
     5701 acggacttga ggatcatgaa ccgcgtcaag taaccttttg acaaaatcag aacgaagtcc
     5761 cgtttccacc ttcatttcct cgggtgtcat gtccctcagc aattcgcaaa accgatcata
     5821 cagatcgtca ggagccattg gatcgtatcg gtaaatctcg attgggattc tacattcaga
     5881 caaatacgat tccatcagct cttgagagcg atctctgcga aggccaccat gttgggcacc
     5941 aagaagggga aaagcgattg attcgatttg tttctcttgg tatgtctgca tgaattttct
     6001 caaaccagcg tgcaagtatt cttctttgct cggttccttc cagtgacgct tcgtcggaaa
     6061 attgaggatc catctgtcgg gagctttgta aatccagagc gagccgattt cgatcatgcg
     6121 gcgtctgcac aaagaaacat attggtcgtg catttctggg taccgtagac ggcactccag
     6181 cgctatgccc gcacccatga ctccgacgca gtttaccgtg ttgacgagcg tctgcgccct
     6241 cgaggtgaag atgttgccat ctataacgct gatgtccgcc atgagccgtt cccttcaaaa
     6301 gaaaaacgac gtgtccacta taacggacac gggacaggtc cagtttatcg ctgatgcgga
     6361 gccttggttt gcgcagatcg cgttggttat gaagcgcggt gtgacgctcg ggaagatcaa
     6421 aacctctgcg caccggcgac gcttaccgtc tgggagcgcg gtccaccatt cagcccgcaa
     6481 cgcatcttcc gatgccgcaa tgatttcgcg gttagtggaa aaggtggttg ttgctgctgc
     6541 ggcgttgccg tcggtgaaga tgtgttggtt gtatcgcaga acatccgggg acacctccaa
     6601 tatcacgagc tcctcctgaa gttctctcct tctgaatagc atcggatttt tggggttgaa
     6661 gtacagcggg gcatactcat ggatggaacg cctgaagacc ggttcgatac ggtttctccg
     6721 ctcttgaact tggggatctg agatatcact cgcggtaagt ccgcgttggt gtgcgcagtg
     6781 ccatgaaagg atcccgtacc ggaagatgtt gcgtaggtta tcccgatggg taatgtggta
     6841 gaaatgtctc cataatacaa ccggcatctg agtccctttc tgcgaaactt ccagacccaa
     6901 gtctttgttt ccgaatgacg ggccggtttg ccccgccctc aatccccctt atatccgatc
     6961 tctttccaca cgcgtttcgc cagcgccagt ccttctttcg tcggcttcag gtcgccggcg
     7021 atgcttgcgg gaaggcgtgt gtaccccttc ttgccggcat gcgaggggaa tggcgacgcg
     7081 ttgtccttcg cgtacttcgt gcgctttgtc tcgttccgga aatccggcac ttccagcggc
     7141 ataccatcct tgagcgcgct cttccacgcg aggatgccga cactgctcat ggccacgccg
     7201 cgatagacgt caaggaacgg ctgcgtgccc gagcgaatcg cgttggcgaa ttcgaagttc
     7261 gtccagaaat cgccgccgcc atgccctgcc ttgcgggcga ggtctgcgtg caccggccag
     7321 tcgggaaggt atatgcgatc cctgagttcc cccggcttca tgtcccactc ctcgtgccag
     7381 acccgcacct gcccgggacc gtagtagccg ggtccccggg tgatttccat cgcccccttg
     7441 gtgccatgca cgcggtacca gttgctgtgt cccggcagcg taaggccgaa aatccggaac
     7501 accgatccgt tatccatgcg gcaaaggatt accgagccgg ggtccccgcg gcgcacggtc
     7561 ttcgtatccg gttcgcggca cgcgatggac agcccgttca ccatcaccgg catcgtatca
     7621 gtgatgaata ccagcggggc gagcgcgtgg gtgcaatagt acgtggatgg tatccaattg
     7681 cgccagtggt tcatgccggg agcgatgcgc aggcggtctt ccggcgccat cggatggttg
     7741 tattccccct cggcgtaggt gacgcggccg atctccccgg tctgatatac ccgccgcatc
     7801 tccatgttga aggcggtgta ggggtagttc tccgccagca tgtagataag cccgctcttc
     7861 tccaccgcac ggcagagagc caccccctcc cccagcgtgg cgttgcagat gcactcgctc
     7921 atcacgtgct tgcccgccgc aagggccttg atggcgaacg gcgcgtgctc gtgaaagtag
     7981 ttggcaagaa tgaccgcgtc catgtcgtgc tcgaggaacc ggtcgtagtc ggtgtaggtc
     8041 gccacgtggt accgcttacc gacctcgagc aggcgctcct cccagagatc gcacagcgcc
     8101 acgagcttca tgccgacgag atcggttgct gacgaggcga acgactgccc acgcccgacg
     8161 ccgac
//
LOCUS       DAOSOO010000149         8149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8789_length_8149_cov_5.662960, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000149 DAOSOO010000000
VERSION     DAOSOO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8789_length_8149_cov_5.662960"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLT86_10335"
     CDS             complement(<1..213)
                     /locus_tag="PLT86_10335"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOT37049.1"
                     /translation="MDTLAKWFLKTGFFLMLFGTILVLLKRHHPAGKLPGDLVLRSGR
                     DRFVLPFGTIGLGALVLTVYLNLLQRY"
     gene            complement(226..495)
                     /locus_tag="PLT86_10340"
     CDS             complement(226..495)
                     /locus_tag="PLT86_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37050.1"
                     /translation="MEADNTAGASAARPARSDGKKKPEPCPAGIEDRCPIPRYRCREF
                     GEPGRHCFELFEEFEAFVLPEKSDDDQDEPARVLPFPRRRRRKSR"
     gene            576..1565
                     /locus_tag="PLT86_10345"
     CDS             576..1565
                     /locus_tag="PLT86_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37051.1"
                     /translation="MAKRQLWLLAALSLFTGIELWVMRILLPVLRRRVGVFPGMGVTE
                     ILQPVVAAVLMAGTAVLLLWMVFGRRSAGSEVPAVRKAVAGLVLLVGLMQVLRVAAGI
                     DDPWLDAARHVIAMCAIVLIAMDALTRGTTFGRASAGVGSLALFSSALYQSVILFYPT
                     SITARWLATFAETMSLFMPWALSVVVTRRYMDDLVAVAGFAFAVVVSVALATAAAPAI
                     HLIFAQATGFRHAVNPVLNVLTVGASVYFVIRSFRSEELPSQVPWAYIWLAASGIQML
                     QAHLQMAGLAALAVLAWTPPLVLPGQTRRWHFEKPEPVPAAAAVPREDEPPTP"
     gene            1581..3272
                     /locus_tag="PLT86_10350"
     CDS             1581..3272
                     /locus_tag="PLT86_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOT37052.1"
                     /translation="MIALVVLASMVFFAVAYRVYGRFLSRSVGIDDTQTTPAVEINDG
                     VDFVPAKSSLLIGQHFSAIAAAGPIVGPILAGLWFGWLPALLWVVVGAVFVGGVHDFA
                     SLIASVRHGSTSIGELLKQYLSPTAHVLFLGFVWLTLVYVIIAFTDITAQTFRVVAQG
                     EAFGPGVAVSSVLYLVLGVLMGVLLYRFKLNLTIATAIFLPLLVLIVWSGGHLPPSVV
                     TFMSSISSAQWNVVLLAYCFVASLLPMWLLLQPRGYLGGWFLYLTIATGLIGALFGGF
                     SIQYPAINMEGLRSLETGRMMFPVLFITIACGACSGFHAIVSSGTTSKQLRRESDARV
                     VGYGAMLLEGLVAVLALATVMMLPKGDAMLKGDPNLIYANGLARYLGLVGVDFSVALA
                     FALLAFSTFVYDTLDVCTRLARYILQELLGWKSRTGAYAATFITLLVPFLFLMATGEK
                     GYLVAWPIFGTSNQLLASLSLLALSVWLYKTGKPYLYAVIPMLFMLVMTLWSLVQLFT
                     PFLAGLPGLLSGAGMKTPVLVAGCCGLPLFVLTLWLVYESYSVLVLKRARENPSR"
     gene            3283..4086
                     /locus_tag="PLT86_10355"
     CDS             3283..4086
                     /locus_tag="PLT86_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOT37053.1"
                     /translation="MQPNHVKEVLKAGGCAVGTMIHRFVYADVGALVKRAGVDFVLID
                     TEHAPLGMESVSLLCIATRAAGVVPIVRVPDSQYHLIARRLDAGALGIMMPRVKTRAE
                     VELIVESAKYPPLGGRGCGIRPVMLDFESTGTLSGDMDWINENTLVCIQIETREAMEN
                     LEEILSVPGVDATIMGPQDLSIALGTPGNHRSRAMELAVDRMIEVCDRYGVAPGIHIR
                     NTELLLDMKRRGMRLLACGFDEGFMLEAMSQTMNLLRGGDGPKAGETTV"
     gene            4213..4971
                     /locus_tag="PLT86_10360"
     CDS             4213..4971
                     /locus_tag="PLT86_10360"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT37054.1"
                     /translation="MRYVRFARGAKIGYGVLSGKTIRLLDRSFLQNARLTGEKVRLSE
                     VRLLAPVVPRTVMAIGLNYADHAKESNMPIPEEPFLFLKTPGCIIGPGDPIRLVVPEH
                     RNDYEAELVVVIRKRAHAVPAEKAHEFILGYTCGNDVSDRDYQKSDSQWMRSKGCDTY
                     GPIGPVITDEIDPHNVRIQTRLNGVVKQDSNTSQMIFPVQRLIEHVTRYVTLVPGDVV
                     FTGTPPGIGPMKAGDTVEVEIEGIGVLKNPVANA"
     gene            4982..6019
                     /locus_tag="PLT86_10365"
     CDS             4982..6019
                     /locus_tag="PLT86_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT37055.1"
                     /translation="MNTLPEVIENETALDNLLTTPSPELVESVRRIPGDVMVLGVGGK
                     MGPTLAILLRRALEEAGSRARVIGVSRFSGPGLRERLHARGVETIPCDLLDPAQLATL
                     PHADTVIYLVGMKFGSTGAENLTWAMNTYLPGMVARHFAGARIVALSTGNVYPLTPVM
                     GGGSRETDPVGPVGEYAQSCLGRERIFSYWTEKTGSPLTLVRLNYAAELRYGALLDIA
                     QKVHAGEPVDLTTGNFNCIWQGDANSIVVRALEIADSPPAVLNLTGPETLSVRRVAQQ
                     FGSLFGLEPVYSGEEAPTALLSNAGYCFRRFGYPHVPAQRMIEWTAHWVKIGGPTLNK
                     PTHFETRDGKF"
     gene            6016..6999
                     /locus_tag="PLT86_10370"
     CDS             6016..6999
                     /locus_tag="PLT86_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37056.1"
                     /translation="MSAVSPVDHADGTIDTALGRVSPGELEWVYPHEHLLISMREFAG
                     ESIANYPGNTEYARREIIAMLREVRAYGVNGLADPTPIGIGRDDAYVRLARSVSEATG
                     VHIFLSTGLYIPRHWPQWAIEETAAQIGERFCAEFERGIGETGARPTFLKAAVDGEVG
                     ANEEKALTACAYAHCRTGAAVHVHATRSRRAVFDVLTACGVHPSRIYIAHADMNTSES
                     EFMWLAERGVRLVMTNWDFPYHMDQNEARRLVRVLIDAGHLDKILISIDFALTMENRW
                     TVGRFTWDNPDRTSYAYLHTGVLPKLEDAVISKAELNVIMHDNPIEMVARK"
     gene            6996..8063
                     /locus_tag="PLT86_10375"
     CDS             6996..8063
                     /locus_tag="PLT86_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT37057.1"
                     /translation="MTKLPMQANAVLRHGIVIPAHPLALTRDRRLDERRQRALSRYYL
                     AAGAGGLAVAVHTTQFAIRKPKIGLLEPVLTLAAEEVARAQPGRDEPIIKIAGVVGRT
                     DQALAEAALAQRLGYDIALVSLAAFRRASDDTLITHCRAVAEVMPIMGFYLQPAVGGR
                     PLGQRFWARFAEIPGAVAVKVAPFNRYHTLDVVKGVAASGRLHEIALYTGNDDNILID
                     LLTPFRVAIPSGAMTTARFVGGLLGHWAVWTTRAVHLLEVAHKLSTLDTIPRDVLTLA
                     AQVTEANAAVFDAANGYAGVIAGVHEVLRRQGLLAGTWCLDPNETLSPGQAREIDRIY
                     HLFPHLTDDEFVKEHLDEWLR"
BASE COUNT         1433 a         2426 c         2647 g         1643 t
ORIGIN      
        1 gtagcgttgg aggagattga ggtacaccgt cagcacgagc gctccgagac caatggtgcc
       61 gaacggaagg acgaatcggt cgcggccgct gcgcaggacc aggtcacccg gcagcttccc
      121 cgcggggtga tggcgtttga gcaggaccag tatggtcccg aagagcatga gaaaaaagcc
      181 tgttttgaga aaccatttcg cgagggtatc catgacgatc ctccttcacc tgctttttct
      241 gcgccgtctg cggggaaacg ggagtacgcg cgccggttcg tcctggtcgt cgtcgctctt
      301 ttcgggaaga acgaaggctt cgaactcctc gaacagctcg aagcaatggc gccccggttc
      361 accaaactcc cggcaccggt aacgcgggat cggacaccgg tcctctatcc cggccgggca
      421 gggctcgggt ttctttttgc cgtcagatct tgccgggcgg gcggcgctgg cgcccgcggt
      481 gttatctgcc tccatggttg acctctttcg ttatcagatt gatcttgtac acgaatataa
      541 cgagcgcgcg ttccccggcg cgggaagagg accctgtggc aaaacgacaa ttgtggcttc
      601 tggcagccct ttcgttgttc accggcattg aactgtgggt gatgcggatt cttctgccgg
      661 ttcttcgccg tcgcgttggc gtgtttcccg gaatgggcgt aacggaaatc cttcagcctg
      721 ttgttgccgc ggtgctgatg gcgggtaccg ccgtgcttct cctctggatg gtgttcggcc
      781 ggcggtctgc agggagcgaa gttccggcgg tccggaaggc ggtggccggg ttagtgcttc
      841 tggtggggtt gatgcaggtg ctccgcgtcg ccgccggaat cgatgacccg tggctcgatg
      901 ccgcgcgcca cgtgatcgcg atgtgcgcca tcgtgcttat cgcaatggat gcactcaccc
      961 gcggaaccac gttcgggcgc gccagcgcgg gagtgggttc gcttgcgctc ttctccagcg
     1021 cgctgtacca gagcgtcatt cttttctacc ccacaagcat caccgcacgc tggctcgcga
     1081 cattcgcgga aacgatgagc ctgttcatgc cgtgggcgct ctcggtggtg gtgacacgcc
     1141 gctacatgga cgacctggtg gcggttgccg ggtttgcctt tgcggtggtc gtttctgtcg
     1201 cgcttgccac ggctgccgcc ccggcgatcc acctcatttt cgctcaggca acggggttcc
     1261 gccatgccgt caacccggtt ttgaatgtgt tgaccgtagg tgcatcagtc tatttcgtga
     1321 tcaggagttt ccgttccgag gaactgcctt ctcaggttcc ctgggcatac atctggcttg
     1381 cggcgtcggg aatccagatg ctgcaggcac acctccagat ggcgggtctt gcggcgctgg
     1441 ccgtgctggc gtggacgccg ccgctcgttt tgcccgggca gaccagacgc tggcacttcg
     1501 agaagccgga acccgtccct gccgccgcgg ctgttccccg cgaagatgaa cccccaaccc
     1561 cgtgaggatg gtcaaaaagg atgatagctc ttgttgtgct ggcgagcatg gtgtttttcg
     1621 ccgttgcgta tcgtgtgtac gggcgctttt tgtcccgttc agtgggaatt gacgacaccc
     1681 agaccacccc ggcggtcgag atcaacgacg gggtggattt cgttccggca aagtcctctc
     1741 tgctcatcgg ccagcacttt tcggccatcg ccgccgcggg ccccatcgtt ggcccgattc
     1801 tggccgggct ctggtttggc tggctgccag ccctcctgtg ggttgtggtg ggggcggtgt
     1861 ttgtgggcgg cgtgcacgat tttgcgagcc tcatcgcgtc ggtgcggcac ggttctacct
     1921 cgattggtga actcctgaag cagtatctct cgcccaccgc gcacgtgctg ttcctcgggt
     1981 tcgtctggct cacactggtg tatgtgatca tcgcattcac ggacatcacg gcccagacct
     2041 tccgcgtggt ggcccagggg gaagccttcg ggccgggtgt tgccgtcagt tcggtgttgt
     2101 acctggtcct cggcgtgctg atgggggtgc tgctctaccg gttcaagctc aacctgacca
     2161 ttgcaacggc gattttcctc ccgcttctcg tgcttatcgt ctggagtggc ggccatcttc
     2221 cgccgtccgt ggtgaccttc atgtcttcca tttcctccgc gcaatggaac gtggtgctgc
     2281 tggcgtactg cttcgtggcg tcgctccttc ccatgtggtt gctgctccag ccgcgcggct
     2341 atctcggagg gtggttcctc tatctcacta tcgccacggg ccttatcgga gcacttttcg
     2401 gcggcttctc gatccagtat cccgcgatca acatggaagg cctgcgcagc ctcgagacgg
     2461 gccgcatgat gttcccggtg ctcttcatca ccatcgcctg cggggcctgt tccgggttcc
     2521 atgccatcgt aagctcggga accacgtcca aacagctccg ccgcgagtcg gacgcgcggg
     2581 tggtcgggta cggcgcaatg ctgctcgagg gcctggttgc cgtgctggca ctggccaccg
     2641 tcatgatgct gcccaaaggc gacgccatgt tgaagggcga cccgaacctc atctacgcca
     2701 acgggctggc gcggtacctc gggctggttg gggtggattt cagcgtcgcc ctcgcgtttg
     2761 cgctcctggc cttttcgacg ttcgtgtacg acacgctgga tgtctgcacc cgcctcgcgc
     2821 ggtacatcct gcaggaactg ctcggctgga aatccaggac gggcgcgtac gcagccacgt
     2881 ttatcaccct tctggtgccg ttcctgttcc tcatggcaac aggagagaag ggatatctgg
     2941 ttgcctggcc gattttcggc acgagtaacc agctcctcgc cagcctctct cttctcgcgc
     3001 tttccgtctg gctttacaaa acgggcaaac cctacctgta cgcagtcatc ccgatgctgt
     3061 tcatgctggt gatgacgttg tggtctctcg tgcaactctt cacgccgttc cttgcggggc
     3121 tgcccggtct tctgagcgga gccggaatga agacgcccgt tcttgttgcc ggctgttgcg
     3181 gcctgccgct ctttgtcctg acactctggc tcgtgtacga gtcctactcc gttcttgtgc
     3241 tgaaacgagc gagggagaac ccctcgcgtt aggaggtctt tcgtgcaacc gaatcacgtg
     3301 aaagaagtgc tcaaagccgg cgggtgtgcg gtcggaacaa tgatccaccg gttcgtgtac
     3361 gccgacgtcg gggcgcttgt caaacgcgct ggcgtcgact tcgtgctgat cgacaccgag
     3421 cacgccccgc tgggcatgga gtcggtgtcc cttctctgca tcgccacgcg cgccgcaggg
     3481 gtggtgccga ttgtccgcgt gccggattcg cagtaccacc tgatcgcacg ccgcctcgac
     3541 gccggtgcgc tggggatcat gatgccgcgc gtgaaaaccc gcgccgaggt ggagctgatt
     3601 gtggaatcgg cgaaataccc gccgcttggc ggccgtggat gcggcatccg gccggtgatg
     3661 ctcgactttg agtccacagg aaccctttca ggcgacatgg actggatcaa cgagaacacg
     3721 ctcgtctgca tccagatcga aacgcgcgaa gcgatggaga acctcgaaga gatcctcagc
     3781 gttccgggcg tggacgcgac catcatgggc ccgcaggacc tctccattgc gctcggaaca
     3841 ccggggaacc accggtctcg cgccatggaa ctggcggtgg accggatgat tgaggtgtgt
     3901 gacagatatg gcgtcgcgcc cggcattcac atccgcaata cggagctatt gctcgatatg
     3961 aagcgccgcg gaatgcggct gctcgcgtgt ggattcgacg aagggttcat gctcgaggca
     4021 atgtcgcaga ccatgaacct cctgcgcggg ggtgatggcc cgaaagcagg cgagacaacc
     4081 gtgtgagtct gaagcatgcg cgggtctgtg atttccgcag gctcccgcgt gaaggcaacg
     4141 tgggtggcac gaccggaatc acagtgggat tgaagtggag ggcggcaacc gcccttcatc
     4201 aaaggagacg aaatgcgata cgtgcgattc gccagggggg cgaaaatcgg ctacggcgtg
     4261 ctttccggga aaacaatccg gctgctcgac cgatcgttcc tccagaacgc ccggttgacc
     4321 ggagaaaagg tgcggctgtc ggaagttcga ctgctcgccc cggtggtgcc gcgcaccgtg
     4381 atggcgatcg ggttgaacta cgccgatcac gcgaaggaat ccaatatgcc gatcccggag
     4441 gagccgttcc tgttcctgaa aactccgggg tgcatcatcg ggccggggga ccccatccgc
     4501 ctcgtcgttc cggaacaccg caacgactac gaggccgaac tggtggtggt catcaggaaa
     4561 cgcgcgcacg ccgtgccggc ggagaaagcg cacgagttca tcctcgggta cacctgcggg
     4621 aacgacgtgt ccgaccgcga ctaccagaag tccgactccc agtggatgcg ttccaagggg
     4681 tgtgacacgt atggcccgat cgggcccgtc atcaccgatg agattgaccc ccacaacgta
     4741 cgcatccaga cgcggttgaa cggggtggtg aaacaggatt cgaacaccag ccagatgatt
     4801 tttccagtgc aacgcctgat tgagcacgtt acgcgctatg tgacgctggt tccgggagat
     4861 gtggtgttca cgggaacacc tcccggcatc ggcccgatga aggccggcga caccgtggaa
     4921 gtggaaatcg agggtatcgg cgtactgaaa aaccccgtgg cgaacgcgta ggaggagatg
     4981 ggtgaacacg ctaccagagg tgattgagaa cgaaaccgca ctggataatc tgttgaccac
     5041 cccgtcgccg gagctcgtgg aaagcgtccg gaggattccg ggggacgtga tggtgctggg
     5101 cgtgggcggg aagatggggc cgacgctggc aattctcctg cgccgtgcgc tggaggaggc
     5161 gggctccagg gcgcgcgtga tcggtgtgtc tcggttttcc ggcccggggt tgcgggagag
     5221 actgcacgcc cgcggggtgg agacgatacc ctgcgacctg ctcgaccccg cccaactggc
     5281 caccctgccg cacgccgata cggtgatcta cctcgtcggc atgaagttcg gttccacggg
     5341 cgcggagaac ctcacgtggg cgatgaacac ctacctcccc ggtatggtgg cgcggcactt
     5401 cgccggggcg cggattgtcg ctctttccac ggggaacgtg tatccgttga cgccggtgat
     5461 gggtggtgga agcagggaaa ctgaccccgt gggcccggtg ggcgaatacg cgcagtcctg
     5521 cctcggaaga gagcggatct tctcgtactg gacagagaaa acgggcagcc cactgactct
     5581 cgtccgcctc aactatgccg ccgagttgcg gtacggcgcg ttgctggata tcgcgcagaa
     5641 agtgcacgcg ggtgagccgg ttgatctcac cacgggcaat ttcaactgca tctggcaggg
     5701 tgacgccaac agcatcgtag tccgcgcact ggaaatcgcc gactctcccc cggcggtgtt
     5761 gaatcttacc ggaccggaaa cgctgtccgt ccggcgtgtt gcgcagcaat tcggcagtct
     5821 tttcggcctc gagccggtgt attccggtga ggaagcaccc accgcgctcc tttccaacgc
     5881 ggggtactgt ttccggcggt tcgggtaccc gcacgtaccg gcgcagcgga tgatcgagtg
     5941 gacggctcac tgggtgaaaa tcggcggccc gacgctgaac aaaccgaccc acttcgaaac
     6001 gcgggatggg aagttttgag cgccgtttca ccggtagatc acgcagatgg cacgatagac
     6061 accgccctcg ggcgtgtcag ccccggggaa ctggagtggg tgtatccgca cgagcacctt
     6121 ctcatcagca tgcgtgagtt cgccggcgaa tccatcgcga actaccccgg caatactgag
     6181 tatgcgcgtc gtgagatcat cgccatgttg cgggaggttc gtgcgtacgg cgtgaatggt
     6241 ctggccgacc cgacgcccat cgggattggc cgggatgacg cgtatgtgag gttggcgcga
     6301 tcggtatccg aggccacggg cgtgcatatt ttcctgagca caggactgta cattccgcgg
     6361 cactggccgc aatgggcgat tgaagaaaca gcagcacaga taggcgagcg gttctgcgcc
     6421 gagttcgagc gcggaatagg agaaaccggc gcgcgcccga cgtttctcaa agccgcggtt
     6481 gacggagagg tgggcgccaa cgaggaaaag gcgctgaccg catgcgcata tgcgcattgc
     6541 cgcacgggag ctgcggtgca tgttcacgca acccgttcgc ggcgagcggt tttcgatgtg
     6601 ctgactgcgt gcggcgttca tccgtcgcga atctacattg ctcacgccga catgaacacg
     6661 tccgaatcgg aattcatgtg gctcgcggaa cgcggcgtcc gactcgtcat gacgaactgg
     6721 gatttcccct atcacatgga tcaaaacgag gcgaggcgcc tggtgcgggt gctcatcgat
     6781 gccggccatc tcgacaagat attgatcagc atcgatttcg cgctcactat ggaaaatcgc
     6841 tggacagttg ggaggttcac gtgggataac cccgaccgta cttcctacgc gtaccttcac
     6901 acgggcgtgc tgcccaagct ggaagacgcc gtaatcagta aagctgaact gaacgtaatc
     6961 atgcacgaca accctatcga aatggtggct cgaaaatgac aaaactcccg atgcaggcaa
     7021 acgcagtgct ccgccacggg atcgtcattc cggcgcatcc ccttgcgctg acgagagacc
     7081 ggcgcctcga cgagcggcgt cagagggcgc tcagccggta ttacctcgcg gccggagcgg
     7141 gtggactggc ggttgcggtt cacacgacgc agttcgccat ccgcaaacca aagatcgggc
     7201 ttctggaacc ggtgctgacg ctcgcggcgg aagaggtggc gagggcacag ccgggccgcg
     7261 acgaacccat catcaagatc gccggcgtcg tcgggcgaac ggaccaggca ctggccgagg
     7321 cggcgctcgc acagcggctg gggtacgaca ttgcgctggt aagtctcgcc gcgttccggc
     7381 gcgcgagtga cgacacgctc attacccact gccgcgcggt tgccgaggtg atgccgatca
     7441 tggggtttta tctgcaaccc gcggtcggcg gacggccgct ggggcagaga ttctgggcgc
     7501 gtttcgccga aatccccggc gcggtggcag tgaaagtcgc gccgttcaac cggtaccaca
     7561 cgctggacgt ggtgaagggt gtcgcggcat cgggacggct gcacgaaatc gccctctata
     7621 ccggcaacga tgataacatc ctgatcgacc tgctcacccc gttccgggtc gccattccgt
     7681 ccggggcgat gactacggca cgtttcgtgg gaggacttct cggccactgg gcggtatgga
     7741 cgacgcgcgc ggttcacctg ctggaagtgg cgcacaaact ctccactctg gataccattc
     7801 cgcgcgatgt gctgaccctc gccgcgcagg tgacggaagc caatgcggca gtgtttgacg
     7861 cggcaaacgg gtatgccggt gtcatcgcgg gtgtacatga ggtgctgagg aggcaggggc
     7921 tgctggcggg aacgtggtgc ctcgacccga acgagacgct ttcgcccggc caggcgaggg
     7981 agattgaccg catttaccac ctgttcccgc acctgacgga cgacgagttt gtcaaagagc
     8041 accttgacga gtggctgagg tagtgcggca ggccgggagt ggcgcctgta attctggatt
     8101 cccgccttcg cgggaatgac gggtccagtc aggggatgtt caggtaggg
//
LOCUS       DAOSOO010000150         8125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8819_length_8125_cov_7.071871, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000150 DAOSOO010000000
VERSION     DAOSOO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_8125_cov_7.071871"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLT86_10380"
     CDS             complement(<1..959)
                     /locus_tag="PLT86_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37058.1"
                     /translation="MSDLVRTGLRSFCLFGASIALLSFLGCPPPSATTSPAEAPAVSQ
                     RVLDSLRADTLMRCRYHRSFAYQYARSGLQQDAITQFQKAILYCKDDPEVERFYAQYL
                     DQWGKRDSAFVHYRKAGDLDTCHVLTHFWLYSFYHDAGDYQAAIRELLTAARNQEDPA
                     IKYRWLRNAADMMASENMKEESCQIYAELQQRDPTDGELAQRMLACVGDDPEKRLSTL
                     RAACMADTTNRDMCRLYAQEEERAGNDATALGIYLRFAREDSNNVGSWETVLRTAKRV
                     NSNEVILLSLRQLARLEPNSAERWGAVADQLGAENRFNEAWALV"
     gene            complement(1107..1781)
                     /locus_tag="PLT86_10385"
     CDS             complement(1107..1781)
                     /locus_tag="PLT86_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37059.1"
                     /translation="MVTAQARALFCPIGWPGLLFSALCGAILLGGIQSASAGESSGET
                     APWRFRVGLATVGVRHLDVVGVGITGEYRHPLAWRGVQSGGEALLMHSGERRIPTREA
                     CNDFFLNDTAYVSHVYEQVGSVGIGGSLYVPVLKGPDHVGFRVPGVMVGLSGGLMFET
                     DRVGLQSSDIQAYYGYSTPSDTDVSLRPYVRPQIVFMQGGFGLSGSAIIFPRFARWTL
                     GVAYAW"
     gene            complement(1880..2719)
                     /locus_tag="PLT86_10390"
     CDS             complement(1880..2719)
                     /locus_tag="PLT86_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT37060.1"
                     /translation="MPPSRRFVLVVFADVHGNLPALEAAAEDAKFFCPDAVVHGGDIV
                     NGPGSREVVAFFLAKGWNGVMGNHEDYVLRCTSPRAPALWRSERFAPVRWARSHLSRR
                     HLDWLAELPNTISPHPETTVVHGSPGDFRAALRSEMDDERAVALYSGLSSRVVICGHT
                     HLPHVREIGDRLYVNAGSCGITLDGDARASYAVLTQLNENWCGEIRRVEYDTRQVLDL
                     ARRTSWLEEGGGVSATMVHEMMTGISWTTPFVRWWTTQCRERTSVDSYREFARMGGMI
                     PLL"
     gene            complement(2790..3890)
                     /locus_tag="PLT86_10395"
     CDS             complement(2790..3890)
                     /locus_tag="PLT86_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37061.1"
                     /translation="MKPVGIGVVGCGNISEVYLKVARNFGILKLVAVADAIPERAQAK
                     AEAHGLSACSVKDLLANPEVEIVLNLTVPAAHAEIGMAALEAGKCVYNEKPLATRRED
                     AQAMLALADRKGLLVGGAPDTFMGAGLQTCRKIIDDGWIGEPVAATAFFMGHGAERWH
                     PDPEFFYKPGAGPMFDMGPYYLTALVSLIGPVKSVCSAARASFQERVIGSQPKAGQII
                     KVETPTHIAGSMEFQSGAIATVVTSFDVWAHSLPNIEIYGTTGSLRVPDPNTFGGQVF
                     VRRFDAKEWVEVPYSHGYSEQNRSIGLADMACALRTGRSFRPNGRLTYHVLDTMHAFL
                     DSARERRHVDLASTCDRPAALPMNLSPGSLGD"
     gene            complement(3943..5358)
                     /gene="gndA"
                     /locus_tag="PLT86_10400"
     CDS             complement(3943..5358)
                     /gene="gndA"
                     /locus_tag="PLT86_10400"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOT37062.1"
                     /translation="MKNCQFGLIGLGVMGQNFVLNVERNGCRVAVYNRTAATTKAFVE
                     GAARGKKIQPAYTLEEFVGALERPRRIMLLVKAGEPVDDMIRRLSPLLAKGDLIIDGG
                     NSFYRHTEKRTHEVEASGLLYLGMGVSGGEEGALWGPSLMPGGSEAAYKLVEPLLKRV
                     AAKAGEDGKPCVAYIGPGGAGHFVKMVHNGIEYGDMQLIAEAYHLLKTVLGLTADDFS
                     RVFAEWNEGELSSYLIELTAKVFAKKDEETGKPLVDVILDSAGQKGTGKWTTQCSLEL
                     GAPVPTITAAVDARLLSAQKQERMHAAGILPGPEPGTKRVPKKALINAVRDALYVSKI
                     CSYAQGMSLLGAASKAHSYQINLGVVAEIWRAGCIIRARLLNDIKTAYDRDAALPNLL
                     LDEFFRDAVATRQAAWRQVLQTGIAHGVPMPAMAASLAYYDAYRSERLPANLIQAQRD
                     FFGAHTFERIDRPGVFHVDWE"
     gene            complement(5355..6113)
                     /gene="pgl"
                     /locus_tag="PLT86_10405"
     CDS             complement(5355..6113)
                     /gene="pgl"
                     /locus_tag="PLT86_10405"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT37063.1"
                     /translation="MNRHCGGKIRLCEARTELAVAAASFVAEVIAGAVNARGFCSLVL
                     AGGNTPREVYSLLAAPPFRETIPWQDVEVFFGDERCVPPDDPQSNYRMAFQTLLSKVP
                     VRAVHRMEGEAVPQTAALRYGEILEGVFGKGVPRFDLILLGMGADGHTASLFPGTDVL
                     KENRNPVAAVWVPKLGSWRITLTLPVINNARTVAVLVSGNDKADALNRVWNASEPDPQ
                     LPVTLVCPVAGELHWFVDGDAARYLRSEGRKDKS"
     gene            complement(6124..7674)
                     /locus_tag="PLT86_10410"
     CDS             complement(6124..7674)
                     /locus_tag="PLT86_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOT37064.1"
                     /translation="MGFVPVLLALFPVTIVFLLLAVRKTPADIAGVIGWLLTVGIAWA
                     FFETPLSVILLASLAGVIASFPITIMVGTSILQITVMQEAGAIARVVTLIKTLSPRDK
                     VAQIMIINVGFGTLLAALGATPVSILPPIMIGLGYSSFAAIALPAIGYDALCTYALLG
                     IPAVVFSGFVGVPVTEAGGYFARYMPVISTCIALGMLWIAGGWRLVCRGVFPAIVAGM
                     TAGIVAIGMNAMGLVTITGIASGVAVVVVMALYLLLLRKPVIDRAVMTDSDRAVERKM
                     PLSAALSPWIILTAFALVVNAPFLPFFGLTFKTWAMPITIVPGNPEKVRLFWQAYFWV
                     LVSTVLALPFLRLSRKQLGATMVKWSHRALRPMLASAVFFAIALVMNHSGKTADWQLL
                     NPAHNMIAVLASSAANSFGAFYPAIAPFLGLFAGFVSGSEASAIAMLTKLHLETAGKI
                     GAVGLVIAAASGIGGGLASVISPAKLQNAAAAIDRIGEEGSVIRTTFVISLIITAVCA
                     IMTLFWAF"
     gene            complement(7862..>8125)
                     /locus_tag="PLT86_10415"
     CDS             complement(7862..>8125)
                     /locus_tag="PLT86_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37065.1"
                     /translation="LLAIVLAIGVYALVFTLLKRRRRTYFERALDLIDELGERREQIA
                     ANLKQFVAEKADASGVSKTLDQALKAIRRELAAVEQSLRKVSK"
BASE COUNT         1742 a         2542 c         2301 g         1540 t
ORIGIN      
        1 acaagtgccc atgcctcatt gaaacggttc tctgccccga gttgatctgc caccgcaccc
       61 cagcgttccg cgctgtttgg ctccagacgg gcaagttgcc gcagcgaaag cagaatcacc
      121 tcgttcgaat tgacccgttt cgccgtacga agcacggttt cccacgaccc gacgttattg
      181 gagtcctcgc gggcgaaacg aaggtaaatc ccaagcgccg ttgcgtcgtt ccccgcccgt
      241 tcttcttctt gcgcgtacaa ccggcacatg tcccggttgg ttgtgtcggc catacaggca
      301 gcacggagcg tcgaaagccg cttttcaggg tcatccccta cgcacgcaag catcctctgc
      361 gcaagttcgc cgtcggtcgg gtcgcgctgc tggagctccg cgtaaatctg gcaggattct
      421 tccttcatgt tctcggaagc catcatgtcc gcggcgttgc ggagccagcg gtactttatc
      481 gcggggtctt cctgattgcg ggccgcggtc agtagctccc gtattgccgc ctggtaatca
      541 cccgcatcgt ggtagaaact gtagagccag aaatgggtaa gaacatggca ggtatccagg
      601 tcccccgcct tccggtaatg gacgaacgca ctgtcgcgtt tcccccactg atcgaggtac
      661 tgggcgtaaa aacgctccac ttccggatcg tccttacagt aaagaatcgc cttctgaaac
      721 tgcgtgatcg catcctgttg cagcccgcta cgcgcatact ggtacgcgaa acttctatgg
      781 taccgacatc tcatcagggt gtcagcacgg agcgaatcga ggaccctctg gcttaccgcg
      841 ggggcttcgg cgggagaggt ggtcgccgag gggggaggac accctagaaa cgacaaaagc
      901 gcgatggatg cgccgaaaag acaaaaagaa cgcaacccgg ttctgacaag gtcagacatg
      961 caaccagccc tcacgatttg ttacgcggcg tctccgggat acggcggctg ccgccggaac
     1021 atccgccctc ccccgcagcc aggagggata ggagaataaa attagaaaaa cgggcgcgcg
     1081 gttccaaggt taccccggga gacaatctac caggcgtacg cgacgccgag tgtccacctg
     1141 gcgaatcgag gaaaaatgat tgcactcccg gaaaggccga aaccgccctg catgaacacg
     1201 atctgcggtc gaacatacgg gcggagagaa acatcggtat cactgggtgt gctgtaccca
     1261 tagtatgcct gtatatcgct tgattgcaac cctacgcgat ctgtttcgaa catcaggccg
     1321 ccggaaaggc caaccatcac acccggaacg cgaaaaccga catggtcggg gcctttgaga
     1381 acgggaacgt agagtgatcc cccgatgcca acgctgccca cctgctcata cacatgcgac
     1441 acgtacgcgg tatcattcag aaaaaaatcg ttgcaggctt cccgcgtggg aattcggcgt
     1501 tcgccggaat gcataagcaa cgcttctcca ccactctgta ccccgcgcca cgccagtggg
     1561 tggcggtact ctcccgtgat gccgactccc accacgtcaa ggtggcgaac accaacggtg
     1621 gcaagcccga cacggaagcg ccagggagct gtttcgcctg agctctcccc cgcagatgcg
     1681 ctctgaattc cgccgagcag aattgccccg cacaaagcag aaaagagcaa gccgggccaa
     1741 ccaatcgggc agaacagagc gcgagcttgt gcggtcacca ttctcaaccg gtaaggcaaa
     1801 tcatacgaaa gcatcaaaaa gatcaaggtg gatataataa agataactca acggcaagcg
     1861 ctcgtgaaga tgagtggacc tagagcaagg gaatcatccc gcccattcgc gcgaactcgc
     1921 ggtaggaatc gacgctcgtc cgttcccggc attgcgtggt ccaccaccgc acaaaaggcg
     1981 tggtccagct tatccccgtc atcatctcgt gaaccatggt ggcagagaca cctccccctt
     2041 cttcaagcca cgaggtgcgc cgcgccaggt caaggacttg ccgtgtgtcg tactccaccc
     2101 gccgaatttc accgcaccag ttttcgttca gttgcgtgag taccgcgtac gatgctcttg
     2161 cgtctccgtc gagggtgatt ccgcacgagc cggcgttgac gtacagcctg tctccaattt
     2221 cgcgaacgtg tggcaggtgc gtgtgcccgc agatgaccac gcgtgaggac aggcctgaat
     2281 agagtgcgac cgcccgctca tcatccatct cgctgcggag tgccgcgcgg aaatcgcccg
     2341 gagagccatg aacaacggtt gtctccgggt ggggtgatat cgtgttcggg agctcggcaa
     2401 gccagtcgag gtggcggcgc gataagtgtg agcgcgccca cctgaccggt gcaaaccgct
     2461 cggatctcca taacgcgggc gcccgggggg aggtacaccg gagcacatag tcctcgtggt
     2521 tgcccattac gccgttccat cccttcgcga ggaagaaggc aaccacttcg cgcgaacccg
     2581 gcccgttcac gatgtcccca ccgtgcacca ccgcatccgg gcaaaagaat ttcgcgtcct
     2641 ctgccgcggc ttcgagggcc gggaggttcc cgtgaacgtc ggcgaacacg acaagaacga
     2701 aacgacgaga gggaggcatg acagtccagg caaacccctc ccatcctgcc ccaaccggat
     2761 ttgtggcagg atggaagggg tgaacaccgt cagtcgccga ggctgccggg cgaaaggttc
     2821 atcggaagtg ccgcgggacg gtcgcacgtg ctggcaagat caacatgacg gcgttcgcgc
     2881 gcagaatcca gaaacgcgtg catggtatcg agcacgtgat aggtgagcct cccgttcggg
     2941 cggaaggagc gccccgtgcg cagcgcacac gccatgtcgg ccagcccgat gcttctgttc
     3001 tgttcgctgt acccatgcga gtagggcact tcgacccatt ccttggcgtc gaaacgcctg
     3061 acgaatacct ggccgccaaa cgtgttgggg tcaggaacgc ggagcgatcc agtcgtgcca
     3121 tagatctcga tattcggaag cgaatgagcc cacacatcga aactcgttac tacggtcgcg
     3181 atggcgccgc tctggaattc catgcttccc gcgatgtgcg taggcgtctc caccttgatg
     3241 atctgtccgg ccttgggctg gcttccaatc actcgttcct ggaacgacgc cctcgctgcg
     3301 ctgcacaccg atttcacggg accaatcagg gaaaccagcg cggtgaggta atacggcccc
     3361 atatcgaaca tgggaccggc ccccggtttg tagaagaact ccgggtccgg atgccatctt
     3421 tcggcgccgt gacccatgaa aaacgcagtt gccgccacgg gttctcctat ccagccatcg
     3481 tcgatgattt tccggcaagt ctgcaatccc gcgcccatga acgtgtccgg cgccccgcca
     3541 acgaggagcc ccttcctgtc cgcaagcgca agcatcgcct gcgcgtcttc gcgccgggta
     3601 gccagaggct tttcattgta cacgcatttg cccgcttcaa gcgcggccat tccgatttcc
     3661 gcgtgcgccg ccggaacggt cagattgagc acgatctcga cttctgggtt ggcaagcagg
     3721 tccttcaccg aacaggcgga caagccgtgg gcttccgcct tcgcctgtgc gcgctcggga
     3781 atcgcatccg caacggcgac cagtttcagg attccgaagt tcctggcaac tttgagatat
     3841 acctcggaaa tgttgccgca cccgaccacg ccaattccaa ctggtttcac gtgtgagtct
     3901 ccttttacgc ggttcgcgct gttgtcccgc cggggtgccg gattattccc agtccacgtg
     3961 aaaaacaccc gggcggtcaa ttcgttcaaa cgtgtgtgcc ccgaagaaat cccgttgtgc
     4021 ctgaatgaga ttcgccggca ggcgttcgct gcggtacgcg tcataatagg cgagagacgc
     4081 ggccatcgcc ggcatgggga cgccgtgggc gatcccggtc tgcagcacct gccgccaggc
     4141 tgcctgcctt gttgcgacgg catcgcggaa gaactcgtcc agaagaagat tcggcaatgc
     4201 ggcgtctcga tcgtacgcgg ttttgatgtc gttcagcagg cgcgcacgga taatgcaccc
     4261 cgccctccag atttcggcta ccaccccgag attaatctgg taactgtgtg cctttgacgc
     4321 cgctcccaga aggctcatgc cctgagcata cgagcagatc ttcgagacgt agagcgcgtc
     4381 gcgcaccgcg ttgatgagcg cctttttcgg tacgcgcttc gttcccggct cgggtcccgg
     4441 caggatcccg gctgcgtgca ttcgttcctg cttctgcgcg ctcagtaggc gagcatcaac
     4501 agccgcagta atcgtgggca cgggcgctcc gagttcgaga gagcactgcg tagtccattt
     4561 gcccgtgcct ttctgacccg cgctgtcaag aatgacatcc acgagcggct tgcccgtttc
     4621 ctcgtccttt ttcgcgaata ctttcgccgt cagttcgatg aggtacgagg agagctctcc
     4681 ttcgttccat tccgcaaaca cgcgcgagaa atcatccgca gtgagtccga ggactgtctt
     4741 gaggaggtgg tacgcctccg caatgagctg catatcacca tattcaatgc cgttgtgaac
     4801 cattttgaca aaatggccgg cccctccggg ccctatatag gccacacagg gtttcccatc
     4861 ctctccggct tttgccgcca cacgcttcaa cagcggctcg acgagcttgt atgcagcctc
     4921 tgaaccgccc ggcatcagac tcgggcccca aagagcacct tcctcaccac cggaaacgcc
     4981 catgccgagg tacagaagcc cactggcctc cacttcgtgg gttcgtttct cggtgtgacg
     5041 gtagaatgag ttcccgccat caatgatgag gtcgcctttt gcgagcaacg ggctgaggcg
     5101 ccggatcatg tcatccacgg gttcgcccgc tttcaccaga agcatgatcc gccgcgggcg
     5161 ttcgagcgcg ccgacaaatt cctccagcgt ataggccggt tggatctttt tcccgcgcgc
     5221 cgccccttcc acgaacgctt tggtcgtcgc ggcagttcgg ttgtagacgg cgactctgca
     5281 gccgttgcgc tccacgttca gcacgaagtt ctgtcccatg acaccgaggc cgatgaggcc
     5341 gaactgacag tttttcatga cttgtccttt ctcccctcgc tgcgcaggta gcgcgccgcg
     5401 tccccgtcga caaaccagtg aagctcgcct gccacgggac aaaccaacgt cacaggaagc
     5461 tgcggatccg gttccgatgc attccagacg cgattcagcg cgtctgcttt gtcgtttccg
     5521 gaaaccagaa ctgccacagt gcgggcgtta ttgatcaccg gcagagtgag cgtgatcctc
     5581 caggaaccga gcttgggtac ccacaccgcc gcaaccgggt tccgattctc tttcaacacg
     5641 tccgttcccg gaaacaggga agccgtgtga ccgtcggcac ccatgcccag tagtataaga
     5701 tcgaaacgcg gaacgccttt cccgaacacc ccctccagaa tctctccgta gcgcaaagcg
     5761 gcggtctgcg gaactgcttc accttccatg cggtgcaccg ccctcaccgg aaccttggat
     5821 aacagtgtct ggaacgccat ccggtagttg ctctgcggat cgtccggagg gacacaccgt
     5881 tcgtccccga aaaatacctc cacatcctgc caggggattg tctcccggaa cggcggcgcg
     5941 gccagcaggg aatacacttc gcgtggcgtg tttccgcctg cgagaacaag cgaacaaaat
     6001 ccccgagcgt tgacggcacc ggcaatcacc tccgcaacga acgacgccgc cgcgacggcg
     6061 agttcggtcc tcgcctcaca cagcctgatc ttgcccccgc agtgccggtt caacggagtc
     6121 ccctcaaaac gcccagaaga gcgtcatgat tgcgcacact gcggtgatga tcagcgaaat
     6181 cacgaacgtg gtgcggatga cgctcccctc ctcgccgata cggtctattg ctgccgctgc
     6241 attctgaagt ttcgccggcg aaatcacact cgccagaccg ccgccgatac cacttgccgc
     6301 agcgataaca aggccgactg caccgatttt ccctgccgtt tcgaggtgca gtttggtcaa
     6361 catggcaatt gccgatgcct ccgacccgct gacaaacccg gcaaaaagcc cgaggaaagg
     6421 cgcaattgcc ggatagaacg ccccgaacga gttcgccgcg gatgatgcca gcactgcaat
     6481 catgttgtgg gccgggttca aaagctgcca gtctgccgtc ttaccggagt ggttcatcac
     6541 cagagcaatg gcaaagaaga ccgcggaagc gagcatcggt cgcagcgctc tgtgggacca
     6601 tttcaccatg gtagcgccaa gctgtttccg actcaatctg aggaatggca gcgccagcac
     6661 ggtgcttacg agaacccaga aatacgcctg ccagaaaagc ctcacctttt ccgggttgcc
     6721 cggaacgatc gtgattggca tcgcccaggt cttgaacgta aggccgaaaa acggaaggaa
     6781 gggcgcgttc acgacaagag cgaacgcggt caggattatc cagggagaga gagctgccga
     6841 aagcggcatt ttgcgttcaa ccgcccgatc cgaatccgtc atcacggccc ggtcaattac
     6901 cggtttcctg aggagcagca ggtagagcgc catgactacc acgaccgcca ctccggaggc
     6961 aatccccgtg atcgtgacca atcccatcgc gttcattccg atcgccacga ttcccgccgt
     7021 catccctgcc acgatagcgg ggaacactcc cctgcaaacc agccgccatc ccccggctat
     7081 ccacagcata cccagcgcga tacacgtgct gatgaccggc atataacggg cgaaatagcc
     7141 acccgcttcg gtgaccggga cacccacgaa acccgagaac acgacagctg gaatgccaag
     7201 aagcgcatag gtgcaaaggg cgtcgtaccc gattgcgggg agggcgatgg ccgcgaacga
     7261 cgagtatccg agaccgatca tgatcggagg caggatcgaa accggagtcg ccccgagcgc
     7321 tgccagaaga gtgccgaaac ccacgttgat gatcatgatc tgggccactt tgtcacgtgg
     7381 cgagagcgtc tttatcagcg taacgacgcg cgcgatcgct ccggcctcct gcatgacggt
     7441 gatctggagg atcgaggtac ccaccatgat ggtgatggga aacgacgcaa tgacgcctgc
     7501 cagactggcc agcagaataa cggaaagcgg ggtttcaaaa aacgcccacg cgatgccgac
     7561 ggtcaaaagc catccaataa cgccggcaat atccgcaggc gtcttccgga ccgccaggag
     7621 aaggaacacg attgtaacgg ggaacaacgc cagcagaacc ggtacgaaac ccatgatgaa
     7681 ttcctcaatg ggggcaacga atgagaaacg acgcaaagaa cactctgtaa gtattctcaa
     7741 tgtcataatt tgggtcaatc cggcggtggc gtatgtggag ggaacgcgga aaggtatgca
     7801 caaaaaaaag cgggacaggc tggtgccgcc tgtcccgctt ccgggtcgcg cgtgcgtgtc
     7861 gctacttgga aaccttcctc agtgattgtt ccactgccgc aagttcgcga cgaattgctt
     7921 tgagcgcctg gtccagcgtt ttcgacacac cggacgcgtc cgccttttct gcaacgaact
     7981 gcttcaggtt cgcggcgatc tgttctctcc gttcccccag ttcgtcgatc aggtccagcg
     8041 cgcgttcgaa gtacgtgcgc cgacggcgtt tgaggagcgt gaacacgagt gcgtacacgc
     8101 cgattgcaag aacgatggcg agcag
//
LOCUS       DAOSOO010000151         8089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8870_length_8089_cov_5.524023, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000151 DAOSOO010000000
VERSION     DAOSOO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8870_length_8089_cov_5.524023"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLT86_10420"
     CDS             <1..536
                     /locus_tag="PLT86_10420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37066.1"
                     /translation="EKTWLLWSRDNGRTWGEHQLVADDINETAYCVLPSGEWLLVGRS
                     NRDKEQSSFVRSSPDGKTWSEPRPFFGGTRHPSDLAVLADGSVLCVFGYRNEPCGARA
                     RRSTDGGMTWSNVELVVHDKATTGDCGYPSVVRWNGWVVMFYYDASKASLFAYDGTGC
                     VVECVRVREEELIAAVR"
     gene            1399..1692
                     /locus_tag="PLT86_10425"
     CDS             1399..1692
                     /locus_tag="PLT86_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT37067.1"
                     /translation="MRPTSDLLRKERAAILQIARRYGARNLRVFGSVARGEDTEMSDI
                     DILVDFAPDVSLFDHAALMEELESALGRKVDVVSEHGLRPRMRERVLKEAVPL"
     gene            1689..2036
                     /locus_tag="PLT86_10430"
     CDS             1689..2036
                     /locus_tag="PLT86_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT37068.1"
                     /translation="MRSDRERLLDALDAINRIREKVTEDLDSFLADEMVQVWVTHHLE
                     ILGESIARLSDELKTHYPRVPWSRAVAMRNILVHHYFSVDPRQVWNTVANDLPKLQRD
                     LEAILKSMPEEST"
     gene            2486..3565
                     /locus_tag="PLT86_10435"
     CDS             2486..3565
                     /locus_tag="PLT86_10435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37069.1"
                     /translation="MATPASNRPAPLAELHVDSLSSAERIRVTKDCGYFPRSVVTRDG
                     AIVIVYRAGAGHMGSGGYLASVRSEDNGRTWSEPVTVVASPPNDDRNPALGIAHDGTL
                     VCAFWRCRLYNSDGTRIPPDQTPKESDAYRTGFAYSTDGGRSWGEPVFAPAGEPWSHY
                     SPFGRILTVRDGRLALPVYLAKKTWLIWSSDNGRTWEDLTLVAEDINETAYCVLPSGE
                     WVLVGRENNGTGMFSLVRWSQDVGRTWSAPVQFLENRRLPSDLVVLCDGSLLAVHGYR
                     TIPRGVRAVRSTDGGRTWGSTDLVLLEGKPNTDLGYPTVEVKDGWVVIAYYDASAAPE
                     DKTDPTGAYLEVVRVREQEIIERVR"
     gene            complement(3933..5180)
                     /locus_tag="PLT86_10440"
     CDS             complement(3933..5180)
                     /locus_tag="PLT86_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT37070.1"
                     /translation="MNTAAFLDDIRSFRGFGRETKVRETRSASFSGEPVSVATFVNEF
                     WTSRQRAAHSLHELSYRACFKPQLPRFFIERLTAPGDHVYDPFMGRGTTPLEAALLGR
                     VPLGCDVNPLSEMLVRPRLHPPQMDEIEQRLAEIDLDAATAVRTDLKVFYHPETLREI
                     CALRDYLHAREQRGELDALDRWIRMVAVNRLTGHSPGFFSVYTLPPNQAVTVAQQRKI
                     NLRRNQKPPRRDVREIILRKTRSLLRDCDDETRRVLANAGKDARFLTQPAGSVPGIPG
                     DSVRLVVTSPPFLDIVNYAQDNWLRCWFCGIDAGSVGITMARKLEEWQAAMSEVFREL
                     ARVLTPSGHVAFEVGEVRTGTVNLEEAVIPCGIAAELSPVCVLINDQHFTKTSNCWGV
                     ENRSKGTNTNRVVVFRKRQAASE"
     gene            complement(5239..6315)
                     /locus_tag="PLT86_10445"
     CDS             complement(5239..6315)
                     /locus_tag="PLT86_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37071.1"
                     /translation="MAARYRVAIVGCGGIAHTHADSCRKMPEVDIVGLADVRPEALAE
                     FGKTFEVPAEQHFTDYLTMYDAVKPDIVCVCTRPAQHCAPTVAALQRGIHVLCEKPMA
                     LDLEEADAMVAASEQSGAKLAINTQRHTDPVYLHAVKLVKEGRIGELRTLRSECKTYE
                     AGYGMMNIGAHYLDAMRLFGGDAAWVFAHLTNAAGNDITPADIEPGERDTGLTAGKAC
                     TMQIGYASGVMGVSEYWVGTATFGFEIVGTKGALAIRGRDASIFVTNGGGGKANDEIV
                     WETIPVPLADEEKEVVAERRWSTIFMMRALIKGIEENAVPACSGYDGRAVLEQIMATY
                     VSQISGSRAKLPLADRRHPLKHWQ"
     gene            6507..7400
                     /gene="era"
                     /locus_tag="PLT86_10450"
     CDS             6507..7400
                     /gene="era"
                     /locus_tag="PLT86_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT37072.1"
                     /translation="MEQNTTGTTRCGYIAIIGPPNAGKSTLMNALVGERLSIVTPKPH
                     TTRTRVLGIITDPNRNAQAIFLDTPGMVAEPRYRLQEAMMEHIHRSIRDADVILAMFD
                     YTRPDPVLIQRTVELAAVPDKAAVFVLNKVDLAEKEPDLSPYPEGTIPVSALLELNLR
                     PVGDAITAHLPEGPFLYPPDTIAEQPERFFVAELIRETIFVTMKEEIPYTTAVVVEEF
                     IERTPKDYINATILVDRDSQKGIMIGRKGQQLKQIGQLSRKKIEDFLGRPVYLELFVK
                     VKRDWMKKDSELRDLGYLERK"
     gene            7641..>8089
                     /locus_tag="PLT86_10455"
     CDS             7641..>8089
                     /locus_tag="PLT86_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT37073.1"
                     /translation="MADAKKYVYLFGGGTAEGNAKMKNLLGGKGANLAEMAAIGLPVP
                     PGFTITTEMCTVYYKLNQRYPAELDEQVKTGIAHIEKIMGKQFGSPTNPLLVSVRSGA
                     RISMPGMMDTVLNLGLNDTTVKGLIKQSGNERFAWDSYRRFVQMYGDV"
BASE COUNT         1566 a         2512 c         2497 g         1514 t
ORIGIN      
        1 tcgaaaagac gtggctgctc tggtcgcgcg ataacggccg cacatggggc gagcaccagc
       61 ttgtcgccga cgacatcaac gagacggcgt attgcgtgct gccgtcgggc gagtggctcc
      121 ttgtcggccg gtcgaaccgg gacaaggagc agtcgtcgtt cgtgcggagt tcgccggatg
      181 ggaagacgtg gtcggaaccg cgcccgttct tcgggggaac gcgccatccg tccgaccttg
      241 cagtgctggc ggatggatcg gttctgtgcg tgttcggcta ccgcaatgaa ccctgcggcg
      301 cgcgcgcccg gcgctcgacg gatggcggaa tgacgtggtc gaatgtggaa ctggtggtgc
      361 acgacaaggc caccaccggc gattgcgggt atccgtcggt tgtgcgctgg aacggctggg
      421 tggtgatgtt ctactacgat gcgagcaagg cgtccctgtt tgcctacgac ggcaccggct
      481 gcgtcgtcga gtgcgtgcgg gtgcgggagg aggagctcat cgcggcggtg aggtaggaaa
      541 agaaagacac gaaagaacac gaaggcgatt gtccggggcg ccgtgcgtcg cacctcaatg
      601 tcgtcattcc tgcgtgctcg ttggagcacg aagcgcaggc gggaatccag atggtgcggc
      661 tcggccaccc acgcccgggg cgatgcccgc aggttggatt atgcccgcct ttcacgcctt
      721 gaaagcggca cttggcaccg gcgaagcccc aacggggcga ggtaacacag cccggctcgc
      781 agggccgggc ggcctcgtgg ctgacacgta tcgccgggtg aggacccgac ggagcgggtc
      841 cctccagaga tatggtgccc ggcgaaatgt tcggtcgtcg agtggcgaac gcggttcgca
      901 cgccgcgttc gacgtatcga gacgacgggc gatggggaga gcggctccgc aaacgtgtca
      961 ggcgattctt cgctgcgtcc gccgcgcagg cggcgctcag aatgacgcgc aagtcgcaat
     1021 agtcattccc gcgcaggcgg gaatccagac gacccgttca ggcctgcaca ccaacgcccg
     1081 tggcgacgcc cgcgggctta gctatatcgg cccttcaggc cttgccaggt gcgtgaaggg
     1141 acgctgtaag ccccaacggg gcgaaatacc acagcccggc tcgcagggcc gggcgacagt
     1201 gccgccgaaa cgcatcgcgg aaccaggacc cgacgtagcg ggtccctccc gcgaggtgtt
     1261 cccgcgccgg tcgttgcggc ggaaacgccc cctgacgtgg ggattctgga gacctcgtcg
     1321 aacgcgggca agagcattca tttggctgca tttctgcgtg attcttcgta atttcgtatc
     1381 agaaacggag gaccacagat gcgaccaact tccgatcttc tgaggaaaga acgcgccgcc
     1441 atcctgcaga tcgcgagaag gtacggtgca cgcaatctcc gtgtgttcgg ctccgtcgcg
     1501 cgcggtgaag acaccgagat gagcgacata gacatccttg ttgacttcgc gccggacgtt
     1561 tcgcttttcg atcatgcggc cctgatggag gaactcgaat ccgcgcttgg caggaaagtg
     1621 gacgtggtaa gcgagcacgg gctgcgaccg agaatgcgcg agagggttct gaaggaggcg
     1681 gtgccgttgt gaggagtgat cgggaacgcc ttctggatgc gctggacgcg ataaacagga
     1741 tccgggagaa ggtgaccgag gacctcgatt cgttccttgc ggacgaaatg gtacaggtgt
     1801 gggtcaccca ccacctcgag attctgggag aatcgatcgc gcggctgtct gacgaactga
     1861 aaacgcacta cccgcgcgta ccgtggtcgc gtgcggtggc aatgcggaat atcctcgttc
     1921 accattactt cagcgttgac ccccgacagg tgtggaacac ggttgccaat gacctcccga
     1981 aactccagcg ggatctggag gccatcctga agtccatgcc ggaagagagt acctgacggg
     2041 gcgtggcatt ttgccgggta ttcaggcatt gaggacccga cggagcgggt ccctccagaa
     2101 ggttggtgtc gcgaggcagg cgcatttatc gtcgccggtg ggtgccgtca ttcccgcgca
     2161 ggcgggaatc cagccggaag ccgtcggtcc ggaatttccg gggtcgtgcg caatggtgca
     2221 cacaacgcgc cttgcctatt ccgcccttgc catgccgctt gtggagtgtg tacggccgca
     2281 aacctggatt cccgcctgcg cgggaatgac ggcttttgtt cgcgggccgg gcgcagcgta
     2341 tcctgcagcc agggcctgac gaagcaggcc cctccaccgg acagccacag tacaaccagg
     2401 tgattgcatg ttcatgtcgc gaatgacccc cttcgctgtc gcatttgccc tcatctcctg
     2461 cgctccccca gtcgaaagga caccgatggc cacgcccgct tccaatcggc ccgcgcctct
     2521 ggccgagtta cacgtcgact ccctctcctc cgccgaacgg atccgcgtga cgaaagactg
     2581 cggttatttc ccgcgttccg tggtcacgcg ggacggggcg atcgtcatcg tctaccgcgc
     2641 cggcgccggg cacatgggtt cgggaggata ccttgcgtcg gtccgctccg aggacaacgg
     2701 gcgcacgtgg tccgagccgg tgacggtggt cgcgtcgccc cccaacgacg accgcaatcc
     2761 ggccctcggc atcgcacacg atggcacact tgtctgcgcc ttctggcgct gccggcttta
     2821 caacagcgac ggcacgcgca ttccgcctga ccagacacca aaggaatcgg acgcgtaccg
     2881 caccgggttc gcctattcga cggacggagg gcgatcctgg ggagagccgg tgttcgcacc
     2941 ggcgggtgag ccgtggtcgc actactcgcc gtttggccga atcctgacgg ttagggacgg
     3001 gcggcttgcg cttcccgtgt atctggcgaa gaagacgtgg cttatctggt cgagcgacaa
     3061 cgggcgcacg tgggaggacc tcaccctcgt ggcggaggac atcaacgaaa cggcgtactg
     3121 cgttctgcca tccggtgaat gggtgctggt gggcagggag aacaacggaa ccggtatgtt
     3181 ctcgctcgtg cgctggtcac aggatgttgg tcggacatgg tcggctccgg tgcagttcct
     3241 cgaaaaccgg cgcctgccca gcgacctcgt cgtgctgtgc gacggatcgc tgctcgcagt
     3301 gcacggctac cgcacgatcc cgcgcggtgt gcgcgcagtc cgttccaccg acggcgggcg
     3361 gacgtggggt tccaccgatc tcgtgctgct ggaaggcaaa ccgaacacgg acctcggcta
     3421 cccgacggtg gaggtgaagg acggctgggt cgtgatcgcc tactatgatg ccagtgctgc
     3481 gcctgaggac aaaaccgacc cgaccggcgc gtatctggag gtggtgcggg ttcgcgagca
     3541 ggagatcatc gagcgggtga ggtgaggcgg aaacgatggg gtaaccacga aatacacgaa
     3601 gtgacacgaa aaacggtggc tgagcctgcg cgagcctgtc gagggcccga cggagcgggt
     3661 ccctcgagcg ttgagccgtc gcacggtgga ggaacggtca tcgagtggcg aacgcggcgt
     3721 gcaactacga acaggggact cgcaactgat ccgcgtcatt cccgcgaagg cgggaattta
     3781 ttctcggcgg caccgatggt gtgcctcaca tcgacagcaa ctcagccggc ttctgcagga
     3841 cgtcacctca ggccacgttg taaggtttcg tgttctggat tcccgccttc gcgggaatga
     3901 cggctttggc agagatcatc aggtactttc catcattctg acgccgcctg ccgctttctg
     3961 aacaccacca cccggttggt gttcgtgccc ttgctgcggt tctcaactcc ccagcagttg
     4021 gacgttttcg tgaagtgctg gtcgttgatc agcacacaca ccggtgagag ttccgccgcg
     4081 atgccgcacg gaatcaccgc ctcctcgagg ttcaccgttc ctgtccgcac ctcacccacc
     4141 tcaaaggcta cgtgcccact gggtgtgagc acgcgggcaa gctcacggaa cacctcgctc
     4201 atcgccgcct gccactcctc cagcttgcgc gccatggtaa taccgacgct gcctgcgtcg
     4261 atcccacaga accagcaccg aagccagtta tcctgagcgt aattcacaat gtccagaaac
     4321 ggtggtgagg tgaccaccag tcgtacggaa tcgccgggta ttccgggaac cgacccggca
     4381 ggttgcgtca gaaaccgcgc atcctttccg gcgttcgcga gcactcgccg tgtctcgtcg
     4441 tcgcaatccc gcagcaggga cctggttttt ctcaggatga tctccctcac atcccgccgc
     4501 ggaggcttct gattccggcg caggttgatc ttccgctgtt gtgcaacagt tactgcctga
     4561 ttcggcggca gcgtgtatac ggagaaaaac cccggcgagt gaccggtgag acggttcacc
     4621 gccaccatgc ggatccaccg gtcgagcgca tcgagttctc cacgttgctc ccgggcatgc
     4681 aggtagtccc gcagcgcaca gatttcgcgc agcgtttccg ggtgatagaa caccttcagg
     4741 tcggtgcgca cggcggtcgc cgcatccagg tcaatctcgg ccagacgctg ttcgatttcg
     4801 tccatctgcg gcggatgcag gcgcgggcgg acaagcattt cgctgagcgg attcacgtcg
     4861 caccccagcg gcacgcggcc cagcaacgcg gcttcaagcg gcgttgttcc gcgccccatg
     4921 aacggatcgt acacgtggtc acccggcgcg gtcaggcgtt cgatgaaaaa gcgcggcaac
     4981 tgtggcttga aacaggcgcg gtaggaaagc tcgtgcaggc tgtgcgcggc gcgctggcgg
     5041 gaagtccaga actcgttcac aaacgtcgca acgctgacgg gttcccccga gaagctcgcg
     5101 gaacgggtct ctctaacttt tgtctcgcgc ccgaaaccgc ggaagctgcg aatgtcgtcc
     5161 agaaatgcgg cagtattcat cacatccggc agcgttacgt ccttcggcgg tcgtgcaagg
     5221 gtggttgctc ccgtgcgcct actgccagtg cttcaatgga tggcggcggt ccgcaagagg
     5281 cagtttcgcc cgcgagcctg atatctgcga tacgtacgtc gccatgatct gttccagaac
     5341 cgcgcgcccg tcgtaccccg aacaggcggg caccgcattc tcttcgatcc ccttgataag
     5401 ggcgcgcatc atgaaaatgg tgctccagcg tcgctccgca acgacttcct tctcctcgtc
     5461 ggcaagcggc acggggatgg tttcccagac gatctcgtcg ttggccttcc cgccgccgcc
     5521 gttcgttaca aagatgctgg cgtcccggcc ccggatggca agcgcacctt tcgttcccac
     5581 gatttcgaag ccgaaggtcg cagtgccgac ccagtattct gacacgccca ttaccccgct
     5641 ggcgtacccg atctgcatgg tgcacgcttt tccggcggtg aggccggtat cccgctcgcc
     5701 cggttcgatg tccgccgggg tgatgtcgtt ccccgctgcg ttggtgagat gcgcgaacac
     5761 ccatgccgcg tcaccgccaa acagacgcat ggcgtcgagg taatgcgcac cgatgttcat
     5821 catcccgtag cccgcttcgt acgtcttgca ctcacttctg agggtgcgca attccccgat
     5881 gcggccttct ttcacaagct tcaccgcgtg aaggtagacc gggtcggtgt gccgctgggt
     5941 gttaatggcg agtttcgctc cggattgttc gcttgcggcc accattgcgt ctgcctcttc
     6001 gaggtcgagc gccatcggct tctcgcaaag cacgtgaatc ccacgctgga gcgcggccac
     6061 cgtcggcgcg caatgctggg cgggccgcgt gcacacgcac acgatgtccg gtttgaccgc
     6121 gtcgtacatc gtcaggtaat cggtgaaatg ctgttccgcg gggacttcga acgttttccc
     6181 gaactccgcc agcgcctccg gacgaacatc tgccagaccc acgatatcta cttccggcat
     6241 cttcctgcac gaatccgcgt gggtatgcgc tatcccgccg caccctacga tcgcaacccg
     6301 gtaacgcgcc gccatgaatg cctcctgaaa gaaaccagtt aacacggaaa acaggccgat
     6361 aacgtgaatg tagcgaccgc cggaagcgcg gacaaagatg gctggcatgt tttgcgaggg
     6421 gcgccgtaat cgttgccggt ctgtatcttt cttccaacca cctgtcccga cattgcaccc
     6481 ctcaatacac ggcgacggaa cacacgatgg aacagaacac cactggcacc acccgatgcg
     6541 gatacatcgc gatcatcggt ccgccgaacg cgggcaaatc gaccctcatg aatgccctcg
     6601 tcggcgaacg gctgagcatc gtcaccccga agccccacac aacccgcacc cgcgttctcg
     6661 gcatcatcac ggaccccaac cgcaacgcgc aggccatttt cctcgataca cccggaatgg
     6721 ttgcggagcc acgctaccgc ctgcaggagg cgatgatgga gcatattcac cgctccatac
     6781 gcgacgcgga cgtgatactc gccatgttcg actatacgcg accggacccg gtgctcatcc
     6841 agcgcacggt ggagcttgcc gccgtgccgg acaaggcagc ggtgttcgtg ctcaacaagg
     6901 tggaccttgc ggagaaagaa ccggatcttt cgccgtaccc ggaagggaca attccggtct
     6961 ccgccctcct cgagctgaac ctccgtcccg tcggtgatgc cattacggca cacctccctg
     7021 aagggccgtt cctctacccg cccgatacca tcgctgagca accggagcgg tttttcgtcg
     7081 ccgagctcat acgtgaaacg atttttgtca cgatgaagga agaaataccc tacaccacgg
     7141 ccgtggtggt ggaggaattc atcgaacgca cgccaaaaga ctacataaat gcgaccattc
     7201 tggtggaccg ggattcccag aaaggcatca tgatcggcag gaagggacag cagctcaaac
     7261 agatcggtca gctttcccgg aagaagatcg aggatttcct gggtcgcccg gtctatctgg
     7321 aacttttcgt gaaggtgaag cgcgactgga tgaagaagga cagcgaactt cgcgatctgg
     7381 ggtatctgga acggaaatag ggcatccctc ccgaccaatt cctgccattt tgacctcaca
     7441 cgccgcgcgg atacttgccg aaacactcac gatttcggta tatttttgca ttctagtggt
     7501 tttgcagcgc acccgacccg gttacccgtg agcaaggcgc tcaacccgcc atctggacca
     7561 tacccggaac gcacaatcac tggaactgaa tacaagcgca caattcacct ttcggtcaac
     7621 aattccacgg aggcaacttc atggcagacg caaagaagta cgtgtatctg ttcggcggtg
     7681 gaaccgctga agggaacgcg aagatgaaga acctgcttgg cggtaagggc gccaacctcg
     7741 ccgaaatggc ggcgattggt ctccccgtac ctcccggttt caccatcaca accgagatgt
     7801 gcaccgttta ctacaagctg aaccaacggt accccgcgga attggacgaa caggtaaaaa
     7861 ccggtatcgc gcacatcgaa aagatcatgg ggaagcagtt cggcagcccg acgaatcccc
     7921 ttctggtgtc cgtccgctcc ggcgcgcgta tctccatgcc tggaatgatg gatacggtgc
     7981 tcaaccttgg cctgaatgac acgaccgtca aaggcctcat caagcagtcc ggaaacgagc
     8041 gattcgcgtg ggatagctac cgccgcttcg tgcagatgta cggcgacgt
//
LOCUS       DAOSOO010000152         8033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8959_length_8033_cov_6.000251, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000152 DAOSOO010000000
VERSION     DAOSOO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8959_length_8033_cov_6.000251"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="PLT86_10460"
     CDS             <1..1611
                     /locus_tag="PLT86_10460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOT37074.1"
                     /translation="ICTETIGWNATSATGVVAAGAAEAVAAGISLLEAGGNAADAAVA
                     TIFALNVTDHGDCSIGGEVPLIIHEARTRQTKVLCGQGRAPRSEDAIAWYMRHGIPDD
                     IKMAPVPSVVDLCITTLQRYGTRSLSDVVAPALALLDAGSEEWQPKLAHTLRRMVEEE
                     RITTGTREEKLQAACDRFYGRHASRNDIADELEAYWIERGGFLRRADLAAHRTTIEEP
                     VRANYRGYNVCKCGPWTQGPFLCQTLRLLEGFDLSAMGRLSPDAIHVATEALKLALAD
                     RDEYYGDPEFVRVPLAALLSDHYTAIRRTLIDMNKASLEVRPGDPDGMKPLKKGGLFR
                     PGVGGTTTCVVADRWGNVVSATPSANVPKTPRDGGKAGVTYGNRLRSLNTTPGHPNCI
                     APGKRPRITLTPTLVLKDGKPVMAISVAGGDLQDQVTLNLLLNAIDYGMAPAAAVTAP
                     RFATAHHQDSFDPDPDRQKTFKETGSLRINDRVSEATREELVRRGHSVHVASGPIGNP
                     VMLAINPETGLFHAAGDPAAGRHAAGLE"
     gene            complement(1619..4300)
                     /locus_tag="PLT86_10465"
     CDS             complement(1619..4300)
                     /locus_tag="PLT86_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37075.1"
                     /translation="MANGQERAPGRTPERNWTIYLVQHSHTDIGYTASQHEMLPAHLQ
                     YLDMALDCCDRTDGYPDEARFRWTCEAAWPVREYIRRRPARQTDRLRARCAEGRIEPT
                     ALMFNMSEITPEAGLVASLQPVREINDALGIRIRTAMQNDVNGVAWAMADYLPPCGVR
                     YLTMGVHRYRSVLPFKVPTAFWWESPSGNRMLAYRADHYLTGNWLGFNPESSLKHWIS
                     MGAADRPALETSLAEYLASLEERGYPYSHLMIQYSGHETDNSHPSTEQCDLIRRWNET
                     HERIVLKSATAGEFLRWVEEHHGQGLPALRVAWPDWWTDGFGSAARETGASRDAHAAL
                     SATNALLAMARMSGADIPEASITRLNEAREQVLFYDEHTFGSVDSVADPESPYTCEQW
                     SEKASYVWQGVQNSALAREEALGYLASVNDAATQPVVRVYNTLNWTRSEPAQVFVPFD
                     VVARGYAVKITDPGTGAACPAQVVYDRPDDRPKETILAYDRPAGRFVTFMAPEVPALG
                     WKDFCLETGPEEDDDAPADPLDVRRIENRWFSLSLDNRTASLRSIRHRPTGRELVDTE
                     SQWRLGQVIYERFPVPEGARHDTGRVFRPEAFRRESARTCRIERQERTALWQRVIFSC
                     DFPGCTNPGGLRVEIRLWHERKQLDLVYHLRKEYTTSPEAVYVAFPFSGNGAELRYDG
                     QGGEVVPGAGQVEGSAADWQTIQHYVRVADGRGQTILSSPRVPLVQLGGLNLGAWRYV
                     PEIPRPHVFSWVMNNYWWTNFRASQGGEWSWTCSLTSTEESSRGTAARFGAECHSPLV
                     ARLERVAKSETRSRIRVTPGNVLAVEMRPERGGDGIILQLRELDGQDTTFRIEAPDSA
                     SAPHMEEVDVTGRPLGTLPDHVPIPGFGVRFVRLALR"
     gene            complement(4404..5231)
                     /locus_tag="PLT86_10470"
     CDS             complement(4404..5231)
                     /locus_tag="PLT86_10470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37076.1"
                     /translation="MAEWAPAASSAVIPEISFRFLQPHFRLPFHAASTRMLRLLRIPV
                     ERVNTRIENVDPFTKQQLRKLCAMPRMSTFAMGVLINRLVTQMAEDEAYVNVGVWHGF
                     TYFAGVLSNGGKQCIAVDNYSSADRFSGQRGIRRLLDYYPRKAKIEAARAAFRRRMAR
                     RGGEHHRLFEMDYREYFQYVHEAPVGVYLYDGDHSYENQLQGLKLAEPFFSKNCVVIV
                     DDCNDEAVYRATRDFIDQSHNSYAILLFQRTCCDRHPTLWNGTMVFQRVPRPHPVPF"
     gene            5498..7753
                     /locus_tag="PLT86_10475"
     CDS             5498..7753
                     /locus_tag="PLT86_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37077.1"
                     /translation="MKQPLKTTISPEHPLLVLMWMPCADPVDEARQLVALWEGLDDVI
                     RPHVSVQIGRHHPERNRVLLAHAQAAGVPITLQIQGDNGDRTDTVPMEEIRALVDSFP
                     CIVGLEIGEASQRTFSDHGSGPEYSMGRNARYARDAIRIAGEHGLYFTWQLQRDSFLA
                     IACSADNEALFDAVCEHRAHVIPMHEMNGEFEKHANHTGAMGMWASGATNAWGVEAQS
                     WYWHDAGYNTPGTYFPGTIEMPGALYAIMFLLGASAGATAYAIEPPRDVWPGGEGAWR
                     FEEWVAPVFRRLILERLIPTREEVLAATPAAYHLPRCRRHRDYRQVLDDLSFDLGEGR
                     LARAAYGVFDRARESEMIPNNARYGWIPALPAKTPREVLDKFPLVLRPGDMRSTEEAR
                     EKLDSIYPPVERGTAWSTVVGPLTLAMNTHENWFVGETARLKVPPPPVHVHIVREAGE
                     LALRWTPNSGDSGYRVWRLRGGVEECVASPGAGVTATILRDAAEGDRYAVSAATPMTE
                     EIEFTLHLHDFLVFSNRESRRSPWIGLDGSVVERPRIGEVPPGAHPDAQDKERRCAAC
                     SPIEDLASPVIAQNDPAREAKRAVSEAIIGWKRALEREDVDAVLTFYDPDYREADGRT
                     VESVGVALRSIFWRYLEESAGSVAAEWGSIYAWSHPAVRIVTRGWHVVSPDVIEVDTK
                     TMMWAGSGPEMEPSDIIRVPWDSPCDLRMTWRWRPGVPPGRAGWRILKTDPAFVRMES
                     TVPFRWCFQGW"
BASE COUNT         1471 a         2549 c         2615 g         1398 t
ORIGIN      
        1 atctgcacgg aaacaatcgg ctggaacgcc accagcgcga ccggtgtggt ggccgcgggc
       61 gcggcggagg cagttgccgc cgggatcagc ctcctcgagg cgggaggaaa cgccgctgat
      121 gccgccgtcg ccacgatttt cgcgctgaac gttaccgacc acggcgattg ttccatcggc
      181 ggggaggtgc cgctcatcat ccacgaagca cgcacccggc agacgaaggt gctgtgcggg
      241 caggggcgcg ccccgcgttc ggaggacgcc atcgcgtggt acatgcggca cggcatccct
      301 gacgacatca aaatggcgcc cgtgccgtcg gtggtggatc tctgtatcac cacgctgcag
      361 cggtacggca cgaggtcgct ttccgacgtg gttgcgccgg cgctggcgct gctcgacgcc
      421 gggtcggagg agtggcaacc gaagctcgcg catacgcttc gcagaatggt ggaggaggag
      481 cgcatcacca cgggaacgag ggaagaaaag ttgcaggcag cgtgcgaccg tttctacggg
      541 cggcacgcga gccggaatga cattgcggac gagcttgagg cctactggat cgagcggggt
      601 gggtttcttc ggagggccga tctggccgcc caccggacca cgatcgagga gccggtccgc
      661 gcaaattacc gggggtacaa cgtctgcaaa tgtgggccgt ggactcaggg cccgttcctg
      721 tgccagacat tgcgtctgct ggaaggattc gacctgagcg cgatggggcg gctctcaccc
      781 gatgcgatcc acgtggcaac ggaggcgctc aagctggcgc tggcggaccg tgacgaatat
      841 tacggcgacc cagaattcgt gcgcgtgccg cttgccgccc tcctgtcgga ccactacacc
      901 gcgattcgcc gcacgctcat cgacatgaac aaggcatccc tcgaagtgcg gccgggcgac
      961 ccggacggga tgaagcccct gaaaaagggc gggctgttcc gccccggggt gggaggaacc
     1021 actacctgcg tggtggcgga tcgctgggga aacgtcgtat cagccacgcc aagcgcgaat
     1081 gtgccaaaaa cgccacggga tggcggcaag gcgggcgtca cgtatggcaa ccggctgcgc
     1141 agccttaaca ccacgccggg ccatcccaac tgcattgcgc ccggcaaacg cccgcggatc
     1201 accctcacgc cgacgcttgt cctgaaagac gggaagccgg tcatggcgat cagcgttgcc
     1261 gggggcgatc tgcaggatca ggtgacgctg aatctcctgt tgaacgccat tgactacggg
     1321 atggcgccgg ctgcggccgt taccgcgccg cgtttcgcca ctgcgcacca tcaggattca
     1381 ttcgaccccg accccgaccg gcagaaaacg ttcaaagaaa cggggtcgct ccgcatcaac
     1441 gaccgggtga gcgaagccac gagggaggaa cttgtccgcc gcgggcattc ggtgcacgtc
     1501 gcctccgggc cgattggaaa tccggtgatg ctggcgatca acccggaaac aggtcttttc
     1561 catgcggcgg gagacccggc ggcggggcgt cacgccgccg ggctggaata ggtcgcgttc
     1621 aacgcagcgc gagacgcacg aatctgacgc cgaaaccggg aatcggcacg tggtcaggca
     1681 gcgtgccgag gggccgcccg gtgacgtcca cctcttccat gtgtggtgcg gacgcactgt
     1741 ccggcgcttc aatccggaac gtggtatcct gcccatcaag ttcgcggagc tggaggatta
     1801 tcccgtctcc tccgcgttcc ggacgcattt ccactgccaa cacgttgccg ggcgttacac
     1861 ggatccgcga gcgggtttcg gattttgcga cccgttccag acgtgcgacc agcggactgt
     1921 gacattccgc tccgaatcgt gccgccgtcc cgcgggagga ttcctccgtc gaggtaaggg
     1981 agcaggtcca cgaccattcg ccgccctgcg aggcgcggaa attcgtccac cagtagttgt
     2041 tcatcaccca cgagaacacg tgcgggcggg gaatttccgg cacataccgc catgcgccga
     2101 ggttcagccc gccaagctga acgagcggca cacgggggct cgaaaggatg gtctgccccc
     2161 ggccgtcggc gactcggaca tagtgctgga tggtctgcca gtcggcggca gagccctcca
     2221 cctggccggc acccggcacc acttcgccgc cctggccatc gtagcgcagt tctgccccat
     2281 tgccggaaaa cgggaacgcc acgtacaccg cttccggaga agtggtgtac tcctttcgca
     2341 ggtggtacac caaatcaagc tgcttgcgct catgccagag acggatttcc acccgcaagc
     2401 cgccgggatt ggtgcatccg gggaaatcgc aggagaagat gacgcgttgc cagagcgcgg
     2461 tgcgttcctg ccgttcaatg cggcaggtgc gcgcggactc ccgccggaac gcctcgggcc
     2521 ggaacacccg cccggtatcg tgacgcgcgc cttccggcac cgggaacctc tcgtagatca
     2581 cctgccccag ccgccactgc gattcagtat ccaccagttc gcggccggtg ggccggtgcc
     2641 gaatactcct gaggctggcg gtgcgattgt cgagggagag cgagaaccag cggttttcga
     2701 tcctgcggac gtccaacgga tccgccgggg cgtcgtcgtc ctcttccgga ccggtttcga
     2761 ggcagaaatc cttccacccg agtgcgggaa cctccggggc catgaaagtg acgaaacggc
     2821 ccgcggggcg gtcgtatgcg agaatcgtct ccttcggacg gtcatcgggc cggtcgtaca
     2881 ccacctgcgc cgggcatgcc gcgccggtgc cggggtcggt gatcttgacg gcataccctc
     2941 gcgccaccac atcaaacgga acaaatactt gagccggttc gctgcgcgtc cagttgagcg
     3001 tgttgtacac ccgcaccacc ggctgcgtgg ctgcgtcatt caccgaggcg aggtaaccca
     3061 gcgcctcctc gcgcgcaagc gcagagttct gcaccccctg ccacacgtac gacgcctttt
     3121 ccgaccattg ctcgcacgtg taaggcgatt ccggatcggc aacgctgtcc accgatccga
     3181 aggtgtgttc gtcgtagaac aggacttgtt cgcgggcttc gttcagcctc gtgatggacg
     3241 cttccggaat atcggcgccg gacatgcgcg ccatggccag cagggcattt gtggcagaca
     3301 acgcggcgtg cgcatcccgc gaagcaccgg tttcccgcgc cgccgaaccg aacccgtccg
     3361 tccaccagtc cggccatgcc accctgagcg cggggaggcc ctgaccgtgg tgttcttcca
     3421 cccaccggag gaactccccg gcggtggcgc ttttcagaac gatacgttcg tgcgtttcgt
     3481 tccagcgccg aatcaggtcg cactgctccg tagaggggtg ggagttgtcc gtctcgtggc
     3541 ccgaatactg gatcatcaga tgcgagtagg ggtatccgcg ctcctccagc gaggcaagat
     3601 actccgccag cgatgtttcc agcgcgggac ggtctgccgc gcccatgctg atccagtgct
     3661 tcaggctgct ttcggggttg aacccgagcc agttcccggt cagatagtga tcggcgcggt
     3721 aggcgagcat ccggttgccc gacggagact cccaccagaa cgctgttggc accttgaatg
     3781 gcaggacgga gcgataccgg tgcacgccca tcgtcaggta gcgaacgccg cacggaggca
     3841 ggtaatctgc catggcccag gcgacgccgt tcacgtcgtt ctgcatcgca gtccggatac
     3901 gtatccccag cgcgtcgttg atttcgcgga cgggttggag cgacgccacg agacccgctt
     3961 cgggagtgat ttccgacatg ttgaacatga gcgcggtcgg ctcgattcgg ccttcggcgc
     4021 atcgggctct caggcggtct gtctggcgcg ccggtctgcg gcggatgtat tcacgaaccg
     4081 gccacgcggc ctcgcacgtc caccggaagc gggcttcatc cgggtaccca tccgtacggt
     4141 cgcagcagtc gagcgccatg tcgaggtact ggaggtgggc gggcagcatt tcgtgctgag
     4201 atgccgtgta cccgatatcg gtgtggctgt gctggacaag atagatggtc cagttacgct
     4261 ccggcgtgcg cccgggggcg cgttcttgtc cgtttgccat ggcgtggtgg cctcaacagg
     4321 gggacgctct gtcgcacgaa agggaatccc gtcgtgtcag tggaaagggt atggagaggc
     4381 ggggcgcgag gcaacgctgc gtgtcagaag ggtaccgggt gggggcgagg cacgcgctgg
     4441 aacaccattg tgccgttcca caacgtggga tggcgatcgc aacaggtgcg ctgaaacaga
     4501 agaatggcgt aggagttgtg gctctgatca atgaaatccc gtgttgcccg gtacacggct
     4561 tcgtcgttgc agtcgtccac aatcaccacg cagtttttgg agaagaacgg ttcggcgagt
     4621 ttcagaccct gcaactggtt ctcgtacgaa tgatcgccgt cgtagaggta aacgccgact
     4681 ggagcttcgt gcacgtactg gaaatactcg cggtaatcca tttcgaacag acggtggtgt
     4741 tcgcctcccc gcctcgccat tctccggcgg aacgccgccc tcgccgcttc gatttttgcc
     4801 tttctcgggt agtaatccag caagcgtctg atgccccttt gtcctgagaa tctgtcggcg
     4861 gaggaatagt tgtccactgc gatacactgc ttgccgccgt tgctcaggac acccgcgaaa
     4921 taggtgaaac catgccatac acccacgttg acgtatgcct cgtcttcggc catctgtgta
     4981 acgagtcggt tgatcagaac ccccatggcg aacgtggaca ttctcggcat cgcacacagt
     5041 ttccgaagct gttgcttcgt aaagggatcc acgttttcta ttctcgtgtt gacacgctcc
     5101 accggaattc gcaggagacg aagcatccgc gtggaagcgg catgaaacgg cagacggaaa
     5161 tgcggctgga gaaaacggaa ggagatttcg ggaatgaccg cgcttgacgc ggccggggcc
     5221 cattcagcca ctgcgcgatt cctccggtag tgcgccggga tacagagctt ccgtaccctc
     5281 agcggtgccc cccacgaaca gccgcacgag acaaaacgcg tcgccgccac gcgacacacc
     5341 tgtccagccc ggcccgactc ctcaatagac aaagtaaacg ctacgaatgt gaaagatgca
     5401 tggagattcc gtgaatgttt atttagattc ccttacgaac tattgcaagc ccctccagcc
     5461 ggggacgcct gatttaccgc ccacgaggta tcccgccatg aaacaaccgt tgaaaaccac
     5521 gatttcccct gagcaccccc ttctcgtctt gatgtggatg ccgtgcgccg atccggtgga
     5581 tgaagcccgc cagcttgttg cgctctggga ggggcttgac gacgtaatcc gcccccacgt
     5641 gtcggttcag attggacggc accacccgga aagaaaccgg gtgttgctcg cgcacgcgca
     5701 ggccgccggt gtgccgatca ccctccagat tcagggcgac aacggcgacc ggacggatac
     5761 ggtgcccatg gaggaaatcc gcgcgctggt ggactcgttc ccctgcatcg tcggactgga
     5821 gatcggggaa gcgagccagc ggacgttcag cgaccacggc agcggcccgg aatactccat
     5881 gggccggaac gcccggtatg cccgcgacgc gattcggatc gccggcgaac acgggctcta
     5941 cttcacctgg cagctccaac gggatagctt ccttgcaatc gcgtgctccg ccgacaacga
     6001 agctctgttt gacgccgtct gcgaacaccg ggcacacgtc atcccgatgc acgaaatgaa
     6061 cggcgaattc gaaaaacacg ccaaccacac aggggcgatg gggatgtggg cttccggcgc
     6121 gacgaacgcc tggggcgtcg aggcgcagtc gtggtactgg cacgacgcgg ggtacaatac
     6181 tccggggacg tatttccccg gcactatcga gatgccgggc gcgctgtacg ccatcatgtt
     6241 ccttctcggg gcaagcgccg gggcgaccgc gtacgccatt gaaccgcctc gcgacgtatg
     6301 gcccggtgga gagggcgcat ggcgattcga ggaatgggtt gcaccggtgt tccggcggct
     6361 gattctcgag cgcctcattc cgacgcggga ggaagtcctc gcggccactc ccgcggcata
     6421 ccatctcccc cggtgccgcc ggcaccgcga ttaccggcag gtgctcgatg atctgagctt
     6481 cgacctcggc gaggggcgcc tcgcacgggc ggcgtacggc gtcttcgacc gcgcacgcga
     6541 atcggaaatg atacccaaca acgcccgcta cgggtggatt cccgcgcttc cggcaaaaac
     6601 cccgcgggag gtcctcgaca aattcccgct cgtgctgcgt ccgggcgata tgcggtccac
     6661 ggaagaggcg cgggagaaac ttgactccat ataccctccg gtggagcgag gcaccgcgtg
     6721 gagcaccgtc gtagggccgc tcacgctggc aatgaacacg cacgagaact ggttcgtggg
     6781 agaaaccgcc agattgaagg tccccccgcc gcccgtgcac gtgcatatcg ttcgcgaggc
     6841 gggtgaattg gccctccggt ggacgccgaa cagcggggat tccggatacc gcgtctggcg
     6901 gcttcgcggc ggggttgagg agtgcgttgc ctcaccgggc gccggtgtta cggccacaat
     6961 cctgcgggac gcggctgaag gagaccggta cgccgtatcc gccgcgacac cgatgaccga
     7021 ggagatcgag ttcacgctcc atctccacga ctttctcgtg ttctcgaacc gggaaagccg
     7081 ccgcagcccg tggatcgggc tggatggctc cgtcgtggag cgtccccgaa tcggcgaggt
     7141 gcccccgggc gcccatccgg acgcgcagga caaggaacgc cgctgtgccg cctgctcgcc
     7201 catcgaggat ctcgcatcgc ccgtgatcgc gcagaatgac cccgcccgcg aggcgaagcg
     7261 cgccgtgtcg gaggcgatca tcgggtggaa acgggcgctg gagcgcgaag atgtggatgc
     7321 cgttctcacc ttctacgacc cggactatcg agaagcggac ggccgcacgg tggagtccgt
     7381 cggcgttgca ttgcgctcga ttttctggcg ctacctcgag gaaagcgccg gcagcgtggc
     7441 ggcggaatgg ggttccatct acgcgtggag ccatccggcg gtgcgcattg tcacaagggg
     7501 ctggcacgtc gtttcaccgg acgtgatcga ggtagacacg aaaaccatga tgtgggccgg
     7561 cagcggcccg gagatggagc ccagcgacat catccgcgtc ccgtgggata gcccctgcga
     7621 cctgcgaatg acgtggcgct ggcggcccgg ggtgcctccg ggccgcgcgg gctggcggat
     7681 tctgaagacc gatcccgcgt tcgtccgcat ggagagcacg gtgccgttcc gctggtgctt
     7741 ccaggggtgg taatgctgcc ctgcgggcag ccggtttggc gcgaccgatg cctgagcgat
     7801 aggttcagag cgatgtaacc cgtatcgccc aacagctcac cgccacaggg gggtgcacaa
     7861 cctgcatgga ggagagcctt ctctcccgct cgctctgcgc gtacctgccc gatcgtgtct
     7921 tcgatggccg gcgcgtcctg ccggatcacg cggtgctggt ggaagaccag cacgtccagt
     7981 gcgtcctgcc ggcgcgggaa ctcaccccgg acacaccaat cgcggcctac ccc
//
LOCUS       DAOSOO010000153         8018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_8980_length_8018_cov_4.071958, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000153 DAOSOO010000000
VERSION     DAOSOO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8980_length_8018_cov_4.071958"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLT86_10480"
     CDS             complement(<1..1430)
                     /locus_tag="PLT86_10480"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT37078.1"
                     /translation="MTTLPDDISPYFSFTFGGTRSRDLLPSWRLETDTVRRDDGLEIV
                     STWAGEPSGIRAIQHLRRFDDNPAVEVMLRLENGSTVKSPVISDILPVDTKLPADAGR
                     KIVLHHAKGSDARWDDFLPLFDELWEGRDFTLAPVGGRSSDSVLPFMNLQLGEGGVVI
                     AIGWTGQWMARFRREGAGIHVAAGMERTHISLEPGESIRTPRILLIPWEGEDRYVGNN
                     LLRKAILAHYTPRKQASGEPVLPPVSHMTMSTYHRTGKVTLEGELGALERAKELGCEA
                     YWIDACWFGTGNWGGETGQWTIRKDVFPDGLRPVGEAAARSDMGFVLWFEPERVCVGT
                     EIERDHPEFVLRGGPNPNFCLLNLGYAPAREFICDRISSIIADSGVTIYRQDFNFQPL
                     PYWRAADPPDRIGISEIRHIEGLYWLWDELRRRHPGLAIDNCASGGRRIDLETTMCSF
                     PLWRSDFSDVGGPGYGRMLQIACQIQT"
     gene            1710..2837
                     /gene="mnmA"
                     /locus_tag="PLT86_10485"
     CDS             1710..2837
                     /gene="mnmA"
                     /locus_tag="PLT86_10485"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT37079.1"
                     /translation="MAGERVIVAMSGGVDSSLAAVLLHEQGYRVEGLTLKLWDRNHAA
                     GLHGAGEAASHESEPAADARAVCERLGVRHHVLDFTEVFEQVVIDDFVREYGSGATPN
                     PCVRCNALVKWEALLRMAEHLDAEFVATGHYARVIHREDGIAELRKGADTSKDQSYAL
                     WRLRQEHLRRTLLPLGGMRKTETRRMAAERGLCTASKPESQEICFIPDNDYVRFLREM
                     DERSGQKSPGFVPGELRTFRSNELAGTHGGTAHFTIGQRKGLRVAFGRPRYVVRMDPS
                     THTVWIGDASDLETATVHAEQVNWSLGPAPDAGTRSTAKIRYLHVGASGRVFPAPGGS
                     MAFVFEEPQRAATPGQSLVVYDHDLVLGGGVIRPVPGEKAS"
     gene            2845..4407
                     /gene="lnt"
                     /locus_tag="PLT86_10490"
     CDS             2845..4407
                     /gene="lnt"
                     /locus_tag="PLT86_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT37080.1"
                     /translation="MNVRSRQVLLSLLSGVLLAAAFPPLPSGPLAWVALVPLLFALDG
                     ADTRRAAGLGYVFGVAFHVCSLYWIAFHVDIPRLLSIAAWVAASLVLGVYYAAACACF
                     RLTARWVGRRWVWFVPFIWVAIEYSRYVSEFAFPWTVIAHSQSRLLGLIQQADFWGVL
                     GVSLWVVALNVLAFRAMQEVPVNARRATGFAATFALVVAASATYGTWRMSENPTVPAR
                     TRIALVQPNIGMDTKWDADEGIRATMDSMMDQTMQIPPGSADLVIWPETAIPDYVVYA
                     PPFSLDSGRNINPRYVAYFDSLTRHLRAPILSGLPAQDFPRDAQFNSAALIIPGTSSV
                     QYYDKTFLVPFGERVPYESAFGFLGKMNLGIARWSPSNRFSVFHSPAGNFGVGICFES
                     VFPRVMSDFVRAGADFLVVVTNDAWFGKTSLIYQHAAFASFRAIENRVWVARCANTGI
                     SAFYDPWGRRVQEAGIFTRETLIGTIGPREKVTPYVLWGDWIVVIAGAVSLIGVAGGG
                     MAAWKRARRRTS"
     gene            4377..6026
                     /locus_tag="PLT86_10495"
     CDS             4377..6026
                     /locus_tag="PLT86_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37081.1"
                     /translation="METCAKTDILTHRRWAWALGAGLAGFIVYFGVWNLAFASDDVAL
                     IYRMRFFGWDGIRPFLLPETGAQGVGYQAYYRPFVVLFTAGEYLFWGTAPGGYHLSNL
                     VVYSLTCSLVFLLVSEVVGSRFVGLATTLLFVVHPLHTMNVAWVSGRTDLFASFFILG
                     SLLAFVHWSESGKKRWIWIGLSAEFLGLCSKEIALLTPFLAVGAEWARVSHQDTHPPV
                     STVLRKTAAWWVLPLLWGAMIAGSSPFLHSFSWAVSVREAFVNLAGSVVLLISPLDYE
                     VLLRWFAERPVLFVCAGALCGAGALFAVWRTQGNRSTMLWGLIWLCVSIVPTYRATMR
                     WYLFLPSVGICMVLASVCASFSERWRVSLVALLVLAFALGLANERRKWAIADAANRSA
                     LSSLLANARGDSIGRVLILTTPAKVRRMPVFGGNTESFFRIHGGTAGGVDVISHLALC
                     DPLGVVKCSRRDATTMTLELTEGEGEFLPLRARAHLTERVVGVGDTIDSDAGTVVVAK
                     LSRTGGVRAVDVSFPRVAFRDSRILLFTRGRFVPFTLPVEE"
     gene            6033..>8018
                     /locus_tag="PLT86_10500"
     CDS             6033..>8018
                     /locus_tag="PLT86_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT37082.1"
                     /translation="MRRSLVLAVLILLEGICGDAGASVGDWRSYSRLVIHRIAAGPDA
                     VWAATEGGIIRSSLVDGSARTYSPSDGLPDRSILSVIVDSAGTAWFGTGEGGILRFTR
                     RKNLSLPVLEPPLLSGQVSRVVALFLWQNRLYVGHSTGVTVLDRGTNRVVENYHQLGA
                     RSDLRNQAVSALAVVGGRIYAGTARGLSWAELDGNPLLPDSWTSVEDSFAGVSSIASF
                     HGKVYVATLNGVKVETTSGWVLTGPAASVYDLAVHDGALWMASSRGLLRTTDGVNWEA
                     IADLSSPVKALAPLEGVALIAAGEAGTYRVQGATPVVTKMPGVDFPPTNAFTNIVLDT
                     SGGVWVAPGPARGAAQYGIFRFRDNTWVHLPPAQNGMPSPPAGEPDGGFISIVVDGQN
                     RIWAGTWGGGIGVVSGEGTTPLYAVTVSTANSPLTGLDGSPDYLVTKAFHSGADGVMY
                     AGIWHSGVFALSPGFAPGSGTTYQIMRKFMENGTISRTLNVTALTLDRYGVLWVGTSS
                     AGVLLIDTNSTPLDGSDDQFVGTLGRQGIDSDVGLRSRNVRSIARARNGEIWVATDAG
                     LTLFQGEFARATNRYSLTTRHFTLDDGLPSNSLNAVLVDSFDVVWVGTDKGLAQVLPT
                     KQVITLPVERLVDTEGKVTSLAFDRARGYLWIGTPSGL"
BASE COUNT         1436 a         2259 c         2549 g         1774 t
ORIGIN      
        1 gtttggatct gacacgcgat ttgcagcatt ctgccgtacc ctgggccgcc cacgtcagag
       61 aaatcgcttc tccagagggg aaacgagcac atcgttgttt cgagatctat acgcctgccg
      121 ccactggcgc agttgtctat tgccaggccg gggtggcgtc ggcgaagctc gtcccacaac
      181 cagtacaggc cttcgatgtg gcggatttcc gaaatgccga ttcggtcggg tgggtcggcc
      241 gcgcgccagt acggcagggg ctggaagttg aagtcctgcc ggtaaatcgt tacacctgaa
      301 tcagcgatga ttgatgagat ccggtcacag atgaattccc tcgcgggcgc gtagccgagg
      361 ttgaggaggc agaagttcgg gttcggtccc ccacggagca cgaactccgg atgatcgcgt
      421 tcgatttcgg ttcccacgca tacacgttcc ggttcgaacc agaggacgaa ccccatgtcg
      481 gatctggccg ccgcttcacc caccggtctg agaccatcgg ggaagacgtc cttcctgatt
      541 gtccactggc ctgtttcgcc tccccagttg ccggttccga accagcacgc gtcgatccag
      601 tatgcttcgc acccgagctc ttttgctcgt tcaagggcgc caagctcgcc ttcgagagtc
      661 accttgccgg tgcggtggta ggtggacatc gtcatgtggg aaacaggcgg aaggacaggt
      721 tcgccgctgg cctgtttgcg gggggtataa tgtgccagga tcgcctttct gaggaggttg
      781 ttcccgacgt aacgatcctc gccctcccag ggaatcaaca ggattcttgg tgttctgatg
      841 ctttcacccg gttccaacga gatgtgtgtc cgttccatac cggctgcgac gtgaatgccc
      901 gcgccttcgc gccggaaccg cgccatccac tggcccgtcc agccgatggc gattacaacc
      961 ccgccttcgc caagttgaag attcatgaag ggcaggaccg aatcggatga cctcccgccc
     1021 actggagcga gcgtgaagtc acgtccttcc catagctcgt cgaaaagggg gaggaaatcg
     1081 tcccagcgtg cgtcgctccc cttcgcgtgg tggagaacga tctttcgacc cgcgtcggcg
     1141 gggagttttg tgtccacggg aaggatgtcg gagataacag gggatttgac cgtacttccg
     1201 ttctccagcc tgagcattac ctcgacagca gggttatcat caaatcgtcg tagatgctgg
     1261 atcgcgcgga ttcccgatgg ctcgccagcc cacgtggaca caatctccaa cccgtcgtcg
     1321 cgtcgcaccg tgtcagtttc gaggcgccag gaaggcagga gatcccggga tcgggtacct
     1381 ccgaacgtaa aggagaagta cgggctgatg tcatccggca atgtggtcac tatgatcccc
     1441 ctgactgtgg cgccattgat ggcatgggaa cgcttcgcgg gcaagcccgg caggctgaag
     1501 ggcgcggtct ttccccgatg agataccagt ataacacgcc cgactctgca ggggcgaggg
     1561 gtgtcaaggg agccgtttca gtggccgcaa tccggaagtt ggccgtctga gctcacgtcg
     1621 gtatctttcc aaagtgtggc attttgagcg agatgcgcac gtctgcaggg caggccgtgg
     1681 tcgctcattt ccggtgtgca ggttgaggga tggctggcga gagggtaata gtagcgatga
     1741 gcgggggtgt ggacagctcc cttgcggccg ttctgctcca cgaacaaggt tatcgcgtgg
     1801 aaggcctcac cctgaagctg tgggatcgaa accacgcggc tggcctgcat ggcgcgggtg
     1861 aagctgccag ccacgaatct gagcctgcgg ccgatgcccg ggcggtgtgc gaacgccttg
     1921 gcgtgcgcca tcacgtgctt gatttcacgg aagttttcga acaggtggta attgatgact
     1981 ttgtccggga atacgggagc ggcgcgacac cgaatccgtg cgtgcggtgc aatgcactgg
     2041 tgaaatggga ggcgttgctg cgcatggcgg aacatttgga cgccgaattc gtcgcaacgg
     2101 gccattacgc aagggtaatt catcgtgagg atgggattgc ggagctcagg aagggagcag
     2161 acaccagcaa ggatcagtcg tacgcgctgt ggagactcag gcaggaacac ctgcggcgaa
     2221 cactgctgcc gctggggggg atgaggaaaa ccgaaacgcg gagaatggct gctgaacggg
     2281 ggctttgcac cgcgagcaaa cccgaaagtc aggagatctg tttcatccct gacaatgact
     2341 acgtgcgctt cctcagggaa atggacgagc gttccggaca gaagtcaccc ggcttcgtgc
     2401 caggtgagtt gcgcacgttc agaagcaacg aactggcagg gacccacggc ggaacagcac
     2461 atttcaccat aggccagcga aaaggactgc gagtagcctt tggccggccc cgttacgttg
     2521 tccgcatgga cccatcgact cacaccgtct ggatcggaga tgcgagcgat cttgaaaccg
     2581 ctactgtaca tgcggagcag gttaactggt ctctcggacc cgcacccgac gccggaacga
     2641 gaagcacggc gaagattcga tacctgcacg tcggggcaag cggccgagtc tttcccgctc
     2701 cgggcgggtc aatggcgttc gtcttcgaag aaccgcaacg agcagcgacg cccgggcagt
     2761 cgcttgtcgt atatgatcat gaccttgttc tcggcggcgg cgtgatcaga cctgttcccg
     2821 gcgagaaggc ttcctgaagg aatcatgaac gtacgttcga ggcaggtgtt actcagtctc
     2881 ctgagcggag tacttcttgc ggccgcgttt ccgccactcc cttcaggccc cctggcctgg
     2941 gtagctctgg ttccattgct cttcgcgttg gatggcgcgg acaccaggcg ggcggcgggg
     3001 ttggggtacg tcttcggcgt cgcgttccac gtgtgttcgc tctactggat cgcatttcac
     3061 gttgacatac cacggcttct cagcattgcc gcatgggtcg ctgccagcct tgttctgggg
     3121 gtctactatg ccgccgcttg cgcctgtttc cgactgaccg ctcgctgggt cggccgaaga
     3181 tgggtctggt ttgtgccgtt catctgggta gcaatcgaat actcgagata cgtgagcgag
     3241 ttcgcgtttc cgtggacggt gatcgcccac tcgcaatcac ggttgctcgg cctgattcaa
     3301 caggcagatt tctggggtgt tctcggggtt tcgctctggg tcgtagcgct gaacgtgctt
     3361 gcgtttcgag cgatgcagga agtgccggtc aacgcgcggc gagccacggg tttcgccgcg
     3421 acctttgcgc ttgttgtagc ggcgtcggca acgtacggta cgtggcgcat gtccgaaaat
     3481 cccacagtac ccgcaagaac tcgcattgcg ctggtgcaac cgaacatcgg aatggatacc
     3541 aaatgggacg cggacgaggg gattcgcgcc acaatggact ccatgatgga ccagacaatg
     3601 cagattcctc ctggaagcgc cgacctcgtg atctggcccg agacggcaat cccggattac
     3661 gtcgtatatg cgcctccctt cagtcttgac tccggaagga acatcaaccc gcgttatgtt
     3721 gcgtattttg acagcctgac gcggcatctt cgcgcgccca tcctgagtgg acttccggct
     3781 caggactttc cgcgtgatgc ccagttcaac tccgcagcgc tcataattcc cgggaccagc
     3841 agcgtgcaat actacgacaa aacgttcctc gtgccttttg gggaacgggt tccgtatgaa
     3901 agcgcgttcg ggttcctcgg aaagatgaat ctgggtatcg cacgctggtc gccgagcaat
     3961 cgcttctcgg tattccattc cccggcgggg aatttcgggg tggggatctg ttttgaatcg
     4021 gtgtttccga gggtgatgag cgactttgtt cgcgcgggag cggatttcct tgtcgttgtg
     4081 accaacgacg cgtggttcgg caagacatcg ctgatctacc agcatgcggc gtttgcctca
     4141 tttcgcgcga ttgagaatcg cgtgtgggtg gcccgctgcg ccaatacggg aatctcagcc
     4201 ttctatgatc cgtgggggag aagagttcag gaagcgggga ttttcactcg agaaacactg
     4261 attggcacta taggtccgcg ggagaaagtc acgccttacg tactgtgggg tgactggatt
     4321 gtcgtcattg ccggcgcagt ctcgcttatc ggcgtagcgg gcggagggat ggccgcgtgg
     4381 aaacgtgcgc gaagacggac atcttgaccc atcgccgttg ggcgtgggct ctcggcgcag
     4441 gtctggccgg attcattgtg tacttcgggg tatggaacct ggcgttcgcg tccgatgatg
     4501 tcgccctgat ttaccgcatg cgtttcttcg ggtgggacgg catcaggccg tttctgcttc
     4561 cggagactgg tgcgcagggt gtgggatacc aggcgtacta ccgcccgttt gtggtccttt
     4621 tcaccgcagg cgaatacttg ttctggggaa cagcgccggg ggggtaccat ctcagcaatc
     4681 tcgtcgtcta cagcctcacg tgttcgctgg tcttcctgct tgtgtcagag gtcgtcggct
     4741 cacggttcgt gggactcgcg actactctcc tgttcgttgt ccacccgttg cacacgatga
     4801 acgttgcatg ggtaagcggc aggacggacc tgtttgcgtc gtttttcatt ctgggatcgc
     4861 ttcttgcatt cgtgcactgg agcgaatcag ggaagaagcg ctggatatgg atcggcctgt
     4921 ccgccgaatt cctggggctg tgcagcaaag aaatcgcgct tttgactccc ttcctcgccg
     4981 tcggagctga atgggcaagg gtttctcacc aagacacaca cccccccgtc tccaccgtgt
     5041 tgcggaaaac ggcggcgtgg tgggtgttgc cgcttctgtg gggagcgatg attgccggca
     5101 gcagcccgtt cctgcattct ttttcgtggg cagtgtctgt tcgagaagcg ttcgtgaacc
     5161 ttgccggttc ggttgtcctt cttatttctc cccttgatta tgaagtgttg ttgcgctggt
     5221 ttgccgaacg accagtgctg ttcgtctgcg cgggtgccct gtgcggcgcg ggtgctcttt
     5281 tcgccgtgtg gcgcacccaa ggtaatcgtt ccacgatgct gtggggactg atctggctgt
     5341 gtgtcagcat tgttcctacc tatcgcgcga ccatgcgttg gtacctgttt ctgccctctg
     5401 tcggtatctg catggttctc gcgagtgtct gcgcttcctt ctccgagagg tggcgtgtct
     5461 ctctggttgc cctgctggtg ctcgcgttcg cgctcggcct cgcaaacgag cgtcggaagt
     5521 gggccatcgc ggatgcggcc aatcgctccg cattgtcctc tcttctggcg aacgcgcgcg
     5581 gcgattccat cggaagggtt ctgattctga caacgcctgc caaggtgcgg cggatgcccg
     5641 tttttggtgg gaatacagag tcgtttttcc gtatccatgg cggcacggcc ggaggggtag
     5701 acgtcatttc gcaccttgct ctctgcgatc cactcggggt tgtgaagtgt tcccggcgcg
     5761 acgcgaccac gatgacactg gaacttaccg agggtgaggg agaattcctg cccttgaggg
     5821 ccagagcgca cctgacggaa cgtgtggtcg gagtagggga taccatcgac tctgatgcag
     5881 gtacggtggt tgtggcgaaa ctctccagaa ccggcggagt ccgtgccgtg gacgtcagtt
     5941 ttccgcgtgt cgcttttcga gactcccgga ttctgctttt cacccgtggc aggttcgttc
     6001 ccttcacact cccagtggag gaatgaggtt tcgtgcgccg ctctctcgta ctagccgttc
     6061 tgatcctcct ggagggtatc tgcggcgatg caggggcatc ggttggcgac tggcgttcgt
     6121 acagccgcct tgtcatccac cgcatcgcgg cgggaccgga tgctgtctgg gcggcaacgg
     6181 aaggaggaat catccgttcg agtctcgtgg acggctccgc acggacgtac agcccgagtg
     6241 acggtctgcc ggatcgaagt atactgtcgg tcatcgtcga cagtgcgggc actgcgtggt
     6301 tcggcaccgg ggaaggaggc attctccggt ttacaagaag gaaaaacctt tcgctcccgg
     6361 ttctggaacc tccgctgctc agtggccagg tatcccgtgt cgtggcattg ttcctttggc
     6421 aaaaccggct ttatgtcggg cactccaccg gagtgacggt tctggaccgt gggaccaacc
     6481 gggtggtgga aaactaccat cagcttggcg cccgcagcga tctgcgcaac caggcagtgt
     6541 cagctctcgc tgtggtggga gggcgaatct acgccggcac cgctcgaggc ctttcgtggg
     6601 cggaacttga tggcaatcca cttcttcctg actcctggac gagcgtcgag gactcttttg
     6661 cgggggtaag ctctatcgcc tcgttccacg gaaaggtcta cgtggcaact ctcaacggcg
     6721 tcaaagtgga aaccacgtcc ggatgggtat tgacgggccc tgcagcgtct gtctatgacc
     6781 tcgcggtgca tgacggagcc ctttggatgg cgtcttcgcg cgggttgttg cggacaaccg
     6841 atggcgtgaa ctgggaagcc atagcggacc tgtcatctcc cgtcaaagcg cttgctccat
     6901 tggagggggt agccctgatt gccgccggag aggcaggaac ctaccgggtc caaggcgcga
     6961 cgcctgtagt aacgaagatg ccgggggtgg acttcccccc tacgaacgcg ttcacaaata
     7021 tcgtgcttga tacctcaggc ggtgtctggg tggcacccgg accggcacgt ggggcggcgc
     7081 agtacgggat atttcgtttc cgcgacaaca cctgggttca tcttccgccc gcccagaacg
     7141 ggatgccatc cccgcccgcc ggggaacccg atggcggatt catttcgatt gtggtggacg
     7201 ggcaaaacag gatctgggca ggaacctggg gcggtgggat cggagtggtt tcgggcgagg
     7261 gtaccactcc gctttacgca gtaacggtga gtaccgccaa cagccctctg acggggctcg
     7321 acggcagccc tgattacctc gtcacgaagg ccttccacag cggcgcggac ggagtcatgt
     7381 acgcgggcat ctggcacagt ggagtgttcg ccctttcccc cggtttcgcc cccggcagcg
     7441 gcaccacata ccagatcatg cgcaagttca tggagaacgg gactatttcg cgcacgctga
     7501 atgtgacggc actcacgctc gaccgatacg gcgtgctgtg ggttggaact tcgtcagcgg
     7561 gtgtgcttct gattgacacg aacagcactc ctttggatgg gtcggacgac cagttcgtcg
     7621 gcacactggg gcgccaggga atcgactccg atgttgggtt gcgaagccgg aatgtgcgga
     7681 gtatcgcacg tgctcggaac ggagagatct gggtcgctac ggatgccggt ttgacgctct
     7741 tccagggcga atttgcccgc gcaaccaatc ggtacagcct gacgacccgc catttcactc
     7801 tcgatgatgg tctgccatcg aacagcctga acgcggtcct cgtggactca ttcgatgtcg
     7861 tgtgggtggg gacggataaa gggctcgccc aggttcttcc cacaaagcag gtgatcaccc
     7921 tgcccgtcga acgccttgtg gatacggagg gaaaggttac ctcgctggca tttgaccggg
     7981 cacggggcta tctgtggatc ggaacgcctt ccggcctc
//
LOCUS       DAOSOO010000154         8003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9003_length_8003_cov_4.890161, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000154 DAOSOO010000000
VERSION     DAOSOO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9003_length_8003_cov_4.890161"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLT86_10505"
     CDS             complement(<1..218)
                     /locus_tag="PLT86_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37083.1"
                     /translation="MNIVVFGAAGWLGRAILTQASGRATIRAFDRGPDAWAEWRDVDG
                     DWTDGEVVHGDIADYPTVARAIAGMDAV"
     gene            544..1011
                     /locus_tag="PLT86_10510"
     CDS             544..1011
                     /locus_tag="PLT86_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT37084.1"
                     /translation="MDPAQDDIKKRLLTFEEGLKAAGIRLTPQRLEIIRVLAESNDHP
                     DAETIHRTLRARMPTLSPDTVYRTLWLLLDLGLITTLGPSRGKARFDGNLAPHHHFVC
                     TKCGLARDFTYPDYDRLSIPDVVKTFGSVERMEVEIRGVCADCARRGGPISRA"
     gene            1088..2550
                     /locus_tag="PLT86_10515"
                     /pseudo
     CDS             1088..2550
                     /locus_tag="PLT86_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
     gene            complement(3010..4044)
                     /locus_tag="PLT86_10520"
     CDS             complement(3010..4044)
                     /locus_tag="PLT86_10520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37085.1"
                     /translation="MRTHLIATKQDRLWQGIPGIEYAANGRLWAALYSGGPKEPHPDN
                     HILLSTSADDGVTWSNPTVTVQPPGSMRAYDPTLWHDPTGRLWLIYNLGDLENGIFPF
                     AAITTDDSTAANPVWSAERRWDLGVGYIIRMNKPTVLSTGEWVLPVSFAREVPDKWFA
                     RETLRQGVAISTDKGASWTLHGEVEAAPWAIETMIVERRDGVLWMLIRTNSGVLWQSF
                     SHDRGLTWSPGEPSCIVNPGTRFYIRRLQSGRLLLINTPVPDARTTMVATVSDVDADS
                     GFPQTGGLLLDGREKVSYPDAVQAPDGTIYAVHDCDRYGPGEVILTVFTEQEVLAAQG
                     NEMPHRLEAK"
     gene            complement(4196..4456)
                     /locus_tag="PLT86_10525"
     CDS             complement(4196..4456)
                     /locus_tag="PLT86_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOT37086.1"
                     /translation="MRSKTHGVSTSDVEVSNISPTGVWLYARGREYFLPFDEYPWFRN
                     ATVGAIHNVELHHGIHLRWPDLDVDLEMDSLSSPEKYPLRFQ"
     gene            complement(4416..4655)
                     /locus_tag="PLT86_10530"
     CDS             complement(4416..4655)
                     /locus_tag="PLT86_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HOT37087.1"
                     /translation="MSPTIFRHGEFRFLFFSREEERVHVHVMGTTGEAKFWLDPKVEL
                     AVNHGLSTGEIREITAIIEGKRREIEDAWREHFGR"
     gene            complement(4794..6320)
                     /locus_tag="PLT86_10535"
     CDS             complement(4794..6320)
                     /locus_tag="PLT86_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HOT37088.1"
                     /translation="MLETVDLSKELDKATYDEWFPKLQDNLRVLQREAFEAGMTTLIV
                     LEGWDASGKGDAVMKLVDKLDPRGFRVDPPAPPSEDEKLRPFLWRFWVKLPPYGAIGI
                     FDRSWYGRVLVERMDGMLQPRQWQQAYQEINQFERQLSDDGQVIIKLFLHISKKEQAK
                     RFKQMEKDPAESWKVTKEDWKHHKQYDDYLRVNEEMLERTSSSYAPWTIIEATDRRYR
                     RVKIFQTVIQAMQAGLERKKFLDARRAEEKEAFKKGLKNGNEQALPLDVMPSILDRVD
                     TSLTYDPEAYKKDLAKLQVRMRELEFECFKHRLPVVIGYEGWDAAGKGGNIKRVTQTL
                     DPRGYNVVPIAAPKGDEATHHYLWRFWRHIPKAGHITIFDRTWYGRVLVERVEGFTAK
                     SDWQRAYQEINEFEQSLVNWGAVVLKFWIHISKEEQLRRFEERQVTEYKQYKITEEDW
                     RNREKWDAYKAALTDVFERTSTSYAPWTIVEGNDKLYARIKTLKTIIGAIEGKIGGKK
                     "
     gene            complement(6468..7766)
                     /locus_tag="PLT86_10540"
     CDS             complement(6468..7766)
                     /locus_tag="PLT86_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT37089.1"
                     /translation="MPRVLIITYYFPPAGGPGVQRIMGLVRHLPSFGWEPAVLTVEGG
                     TFFNRDEESVSRVPPSVPVYRTKAFEPFALYNRLRGKPADEALPIGTVGQGGGGVATR
                     VSRWVRANLFVPDARIGWIPFAVSAAKRIVKHERIDAILTSSPPQTVHLIGRKVARAA
                     GLPWIADFRDPWTQIYYNAELPRCNAARRLDLRLEQGVVRSASELVMINEMVRESLGP
                     AASRGHIIPNGYEEEDFAGELAPTTERFELVYSGNIIPLHAPGPFLEALASLRAADPE
                     FRAAAKLVFVGTVHPAVREQIEHVGLAGVAEFTGFVPHAEAVRRLRTATVALFIGPAN
                     LLTSKIFEYLAAGRPMLALAPPGGDVDRLLRSVGREPVVPADDVGAIRAELERLFGLW
                     KRNSLPVRAPMEGVEHLSRRSQTGQMARLLNRACGVSGNE"
BASE COUNT         1567 a         2452 c         2452 g         1532 t
ORIGIN      
        1 atgacggcgt ccatgccggc aatggcccgc gcgacggtgg ggtagtcggc gatgtccccg
       61 tgaactacct cgccgtccgt ccaatcgcca tcaacgtcac gccattcagc ccaggcgtcg
      121 ggtccgcggt caaatgcccg gatggtcgcc cgcccggatg cctgtgtgag aatggccctg
      181 ccgagccacc cggctgctcc gaataccacg atgttcacgg tgattccccc ttgtgcgcgt
      241 gtgaggagag cacacgcagg ccacgctcgc ccgcaattcg ctgtcacgca ccggcagcct
      301 cgtgcagtgg aggcgatccg ccgctacggc atcagcagtc ctccctcgcc ggcccgtctc
      361 ttgattaggc aaaacgtcgt cttcgtgtca aggggaaact gcggaaaatt tcgtaacgag
      421 gttggtgcgg aaaaccttgc gcgcttcggg cggcaagcct tgcagcgtct cgttcttcat
      481 gctatgttct tatttagtaa ttattcctat ctaatttttt ttcttattct tgaaaggggc
      541 agtgtggacc ctgcacagga tgatatcaaa aagcggttgc tcacctttga agagggcctt
      601 aaggcggcgg gcatacgcct gaccccccag cgactggaga tcatccgggt gctggcagag
      661 tcgaacgacc acccggatgc ggagacgatc catcgtaccc ttcgtgccag aatgccgact
      721 ttgtcgccgg acaccgtgta ccgcaccctt tggctgctcc tcgatctcgg gctcatcacc
      781 acactggggc catctcgggg aaaggcgcgg ttcgatggga atctggcgcc tcaccaccac
      841 tttgtctgca caaagtgcgg tcttgcgcgc gatttcacgt acccggacta tgaccggctg
      901 agcattccgg acgtggtgaa aacgttcgga agtgtcgaaa ggatggaggt ggaaatccgg
      961 ggcgtctgcg ccgattgtgc gcggagaggt ggtccgattt cacgcgcgta agtcactcgc
     1021 ggcgccgcgg cgtggctgtg gcgcctcagg cttcatcacc gattgcgcca accaaggagg
     1081 gaagaccatg cccaggaaaa agaaactcac cacggtggtg ggggcacccg tcgttgacaa
     1141 ccagaacctg atgacggcag gcccgcgggg acccggactg ttgcaggacg tctggttcct
     1201 cgaaaagctg gctcatttcg accgggaagt gatcccggag cggcgtatgc acgcgaaggg
     1261 atcgggcgcg ttcggcacgt tcaccgtcac gcacgacatc accaaataca cgaaggccaa
     1321 gattttctcg aaaatcggca agaagaccga ctgcttcgtg cgtttttcca cggtggccgg
     1381 tgagcgcggc gcggccgatg ccgagcggga catccgcggt ttcgccgtca agttctacac
     1441 cgaggaaggc aactgggatc ttgtcgggaa caacacgccg gtgttcttcc tgcgcgaccc
     1501 gctcaagttc ccggacctca accatgcagt gaagcgcgac ccaaaacgaa cctgcgcagc
     1561 gcgaaaaaca actgggattt ctggagttcg ctgccggaag cgctccatca ggtcacgatc
     1621 accatgagcg accgcggtat cccggcgtcg taccgccaca tgcacgggtt cggcagccac
     1681 acgttcagct tcatcaatgc gaaaaacagg cgctactggg tgaaattcca ctttgtgtgc
     1741 cagcagggaa tcaaaaacct gaccgacgcg gaggccgagg ccatcatcgc caaagaccgg
     1801 gaaagccacc agcgcgacct gtttgacgcc atcgaaaagg gcgatttccc gaagtggaag
     1861 ctgttcgtgc agatcatgac ggaagagcag gcggcgaaaa tgccgtacaa cccgttcgac
     1921 ctcacgaagg tgtggtatca caaggattac ccgctcatcg aagtaggtat catggaactt
     1981 aacaggaacc cggagaacta cttcgcggat gtcgagcagg ccgcgttcaa cccggcgaac
     2041 gtggtgccgg gaatcggatt ctcacccgac aagatgctgc agggccggct cttctcgtac
     2101 ggcgacgcgc agcggtaccg gctgggcgtg aaccacaacc tcatccccgt gaacgcgccg
     2161 cgctgcccgg tgaacagcta ccaccgcgac gggcagatgc gtgtggacgg caacgccgga
     2221 gccactctgg ggtacgaacc gaacagttac ggcgagtggc aggagcagcc ggagttcgcc
     2281 gagccgccgc tcaagatcac cggagcagcg gatcactgga acttccgcga ggatgacagc
     2341 gactactaca cccagccggg gatgctgttc cgcatcatga cagcggcgca gaagaaggcg
     2401 ctgtttgaga acaccgcacg ggccatgggc gacgcgccga aggagatcaa ggtgcggcac
     2461 atcggcaatt gcatgaaggc cgacccggcc tacggcaagg gcgtcgcggc ggcgcttggg
     2521 attcccctga aggacgttcc gaagaagtaa ggggaagtcc gcgaaaatgc cggtaggggc
     2581 gacgcatgcg tcgcccctac ggtaaaacgc gatccaaaca cgggcggaca caggggtccg
     2641 ccactacggt gttttcggga gattgcacgt cctgaggctg tcattccgtg tgcgcgttcg
     2701 cgcacgcagc gaaggcggga atccagccca ccctgacgcg gcggcctcgg cgagaccgcc
     2761 cctaccgaaa aacgcgatcc aaacacgggc ggaaacaggg gtctgcgtgc cgcgagcggc
     2821 acgacgcccc tacacctcaa acacacgcaa caaaacccta cgaagatcca gggggcgcgt
     2881 tgaaacgtgc ccagaccccg ccggcgtttc gacaggttcc cgttcgcggg aacaggctca
     2941 acgaccggcg catcgcacac gcacggcgcg cgacggggtg aagagggaga aaggccctca
     3001 ccctttcagc tatttcgcct ccagccggtg gggcatttca tttccctgcg cagccagcac
     3061 ttcctgctcg gtgaacacgg tcaggatgac ctcgcccgga ccgtagcggt cgcaatcgtg
     3121 cacggcgtag atcgtgccgt ccggcgcctg cacggcatcg gggtaggaga ccttctcgcg
     3181 gccgtcgagc agaaggccac ctgtctgggg gaaaccgctg tcggcgtcca catcgctgac
     3241 cgtggcaacc atcgtcgtgc gggcatccgg aacgggagta ttgatgagca gcaggcgtcc
     3301 cgattgcagg cggcgaatgt agaaccgcgt gccggggttg acgatgcagg aaggctcacc
     3361 cggcgaccac gtgaggccgc gatcgtgaga aaagctctgc cagagaacgc cgctgttggt
     3421 gcggatcagc atccagagca ctccatcgcg acgctcgacg atcatcgtct cgatggccca
     3481 cggcgcggcc tctacctcac cgtggagcgt ccagcttgcg cccttgtccg tggagatcgc
     3541 aacgccctgc cggagcgttt ctctggcaaa ccatttgtcg ggaacctccc gcgcgaagct
     3601 taccggcagc acccattccc cggtggaaag cacggtgggt ttgttcatgc ggatgatgta
     3661 gccgacgccg aggtcccacc gccgttccgc gctccagacg ggattcgccg cggtcgagtc
     3721 gtcggtggta atagcggcaa acgggaagat gccgttttca aggtcgccga ggttgtaaat
     3781 gagccacagg cggccggtcg ggtcgtgcca gagggtcggg tcgtacgcgc gcatggagcc
     3841 cggtggctgc accgtgacag tcgggttgct ccaggtgacg ccgtcgtccg cgctggtgct
     3901 gaggaggatg tggttatcgg ggtgcggctc tttcggcccg ccgctgtaca gggctgccca
     3961 gaggcggccg ttggcggcgt actcgatgcc ggggatgccc tgccagagtc ggtcctgctt
     4021 cgttgcgatc aggtgtgtgc gcacggggaa tcctttcgcg ggcgtggaaa aaggcggcgt
     4081 tccggtaccc ccgaaaggga atgcaaagac gccggtgagt caaggaacaa gcaggtcgcg
     4141 cgaaacgacg tggtgtgcaa cgaaccaaca aaatccgtcg ccaagcagca cggcgtcact
     4201 ggaatctgag agggtacttc tccggagaac tcagcgaatc catttcgaga tcaacgtcga
     4261 ggtcgggcca gcgcaggtgg ataccgtgat gaagctccac gttgtggatc gctcctaccg
     4321 tcgcattccg aaaccacggg tactcgtcga aaggaaggaa atattctcgg ccgcgtgcat
     4381 acagccacac gccggtgggc gagatattgc tgacctcaac gtccgaagtg ctcacgccat
     4441 gcgtcttcga tctcacgtct tttaccctcg atgatcgctg taatttcccg tatctctcca
     4501 gtagagagac cgtggttgac cgcaagctcg accttcggat caagccagaa cttggcttcg
     4561 cctgttgtgc ccattacatg cacgtggact cgttcctctt cgcgggagaa gaacaaaaat
     4621 cggaattcac cgtgcctgaa tatcgtcggg ctcatgccat tagtatctca ctttttccgt
     4681 tacggcgcaa atccgaacgt tcttcctgtt cgagacgcca cacccgcacc acacgacaag
     4741 ggggcgcctt gcgggcgccc cctcagagaa tcagccggac cgcgtccgcg ggtctatttc
     4801 tttccgccga ttttcccctc aatggctccg ataatggttt tgagcgtttt gatgcgcgcg
     4861 tagagcttgt cgtttccctc gacaatcgtc cacggcgcgt agctggtgct ggtgcgctca
     4921 aacacatccg taagagcggc cttgtacgca tcccatttct cgcggttgcg ccagtcttct
     4981 tccgtgattt tgtactgctt gtattcggtt acctgccgtt cttcgaaacg gcgaagctgc
     5041 tcttccttgc tgatgtgaat ccagaatttc agcaccacgg cgccccagtt caccaggctc
     5101 tgttcgaact cattgatttc ctggtacgcc cgctgccagt cgcttttcgc ggtgaatcct
     5161 tccacccgtt ccaccagaac acgcccgtac cacgtgcggt cgaagatggt gatgtgcccg
     5221 gctttcggaa tgtgtcgcca gaaccgccag aggtagtggt gagttgcctc atcacccttc
     5281 ggcgctgcga ttggcacgac gttgtatccg cgcggatcga gcgtctgtgt cacccgtttg
     5341 atgttgccgc ccttgcccgc ggcgtcccat ccttcgtagc cgatcaccac ggggagacgg
     5401 tgtttgaaac actcgaactc gagttcgcgc atccgcacct ggagcttggc gaggtccttc
     5461 ttgtacgcct cggggtcgta ggtgagcgag gtatccaccc ggtcgaggat ggacggcatg
     5521 acgtccagcg gaagggcctg ttcgttcccg ttcttgaggc ctttcttgaa cgcttctttc
     5581 tcttccgctc tccgtgcgtc caggaatttc ttcctctcca gacccgcctg catggcctga
     5641 atgacggtct ggaagatttt cacgcggcga taccgacgat cagtcgcttc gatgatcgtc
     5701 cacggcgcgt agctggaact cgtgcgctcg agcatttcct cgttgacgcg caggtaatcg
     5761 tcgtactgct tgtgatgttt ccagtcctct ttggtcactt tccacgattc ggcagggtcc
     5821 ttttccatct gcttgaaacg ttttgcctgt tccttcttgc tgatgtgcag gaacagtttg
     5881 atgatcacct gcccgtcgtc ggaaagttgc cgttcgaact gattgatctc ctgatacgcc
     5941 tgttgccact ggcgcggctg caacatcccg tccatgcgct cgaccagcac acgcccgtac
     6001 cacgagcgat caaaaatccc gatcgcgccg tacggaggga gtttgaccca gaaccgccag
     6061 aggaacgggc ggagtttctc gtcctcactg ggaggtgccg gtggatccac ccggaagccg
     6121 cggggatcca gtttgtccac cagcttcatc accgcatcgc ctttgccgga ggcgtcccat
     6181 ccctcgagaa cgatcagcgt tgtcattccg gcttcgaatg cttcccgctg caacacgcgg
     6241 agattgtcct gcagcttcgg gaaccattcg tcgtacgtcg ccttgtcgag ttcttttgac
     6301 aggtcaacag tttccagcat gtccccctcc ttggaaggta tggcggaact ccgtgatcat
     6361 gtgacaagca actcgaagcg gttgataggt acaaatcggc acgtcaaggg taccacattt
     6421 cgtacgacaa ggcaaacgcc tgggattgga tgagaaccgt ggagagatta ctcgtttccg
     6481 gaaacgccgc acgcgcggtt gagcaagcgc gccatctggc ccgtctggct tctgcgggag
     6541 aggtgttcca ctccctccat cggtgcgcgc acaggtagcg agttacgttt ccagagacca
     6601 aacagacgct ccagttccgc ccggattgcc cccacgtcgt ctgccggaac gacaggttcg
     6661 cggccgacac tccggagcaa acggtccacg tctccaccgg gcggcgcgag ggcaagcatg
     6721 gggcggccgg cggcaaggta ctcgaagatt ttcgaggtca acaggttcgc cgggccaatg
     6781 aacagcgcca ccgtcgccgt acgaagccgc cgcacggctt cggcgtgcgg cacaaacccg
     6841 gtaaactcgg caacaccggc aagcccgaca tgttcgatct gctccctgac ggcgggatgg
     6901 acggtaccca cgaacacgag cttcgctgcg gcgcggaact ccgggtctgc cgccctcaaa
     6961 gaggccagcg cttcgaggaa ggggcctggg gcgtgcagag ggataatgtt gccgctgtag
     7021 acaagctcaa accgctccgt cgtgggagcc agttcgcccg cgaaatcctc ctcctcatat
     7081 ccattcggga tgatgtgccc acgggaggcc gctggcccca gcgattcgcg caccatctcg
     7141 ttgatcatca ccagctcgga agcagaacgc accactccct gctcgagtcg caggtcaagc
     7201 cgcctggccg cgttgcaccg cggaagctcg gcgttgtagt agatctgcgt ccacgggtcg
     7261 cggaaatcgg caatccaggg cagcccggcg gcccgggcta ccttgcggcc gatgaggtgc
     7321 accgtctgcg gggggctgga cgtgaggatc gcgtcaatgc gttcgtgttt cacgatgcgt
     7381 ttcgcggccg agacggcgaa ggggatccag ccgatccgcg cgtccggcac aaacaggttc
     7441 gcccgcaccc agcgcgacac gcgggtggca acgccgcctc ctccctgccc gaccgtgccg
     7501 atgggcagtg cttcgtccgc tggtttgcca cggagccggt tgtagagcgc aaacggctcg
     7561 aaggctttcg ttcggtacac gggaaccgaa ggcgggacgc gggagacaga ttcctcgtcg
     7621 cggttgaaga aggtgccgcc ttccacggtc aggacggccg gttcccagcc gaacgacggc
     7681 agatggcgca ccagacccat gatgcgctgc acaccggggc cgccggcggg ggggaagtag
     7741 taggtgatga tgaggacgcg aggcatggca gtttctgcga tcagcattga acggtcatct
     7801 cgatacaacc gccgcccgtg ggcggccgtc gctcgatgac tgtccgcgtc agttttcgag
     7861 cggtacccgt gcacagaatg taccaccaag ggatgcgact gtgcgcaaaa tccctttgac
     7921 aggctcgtcg acaggacgat cggcacggtt ggcggcaata cgtccgatag gacacatggg
     7981 gcgtttggtg cctgggcggg gga
//
LOCUS       DAOSOO010000155         7880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9193_length_7880_cov_5.141214, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000155 DAOSOO010000000
VERSION     DAOSOO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9193_length_7880_cov_5.141214"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLT86_10545"
     CDS             <1..631
                     /locus_tag="PLT86_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT37090.1"
                     /translation="RNAVSWAVILFAAGVSLSWLLPIRFALMATVIALALVWYAGKLK
                     RTILWGHILVAIISGSAFVFGGMLGTRLAPAFAPAFLAAAFHLGREMLKAAADYPGDM
                     EQGYDTLAVRRGVERTCRLASIPLAGVIALSPIPFIIGWFGFCYLVSVIILIDGLLAY
                     VIARIWQSPSSETAARMATLLKWGMVAGIATVWGDVWTRSLTGVMLTLR"
     gene            589..1389
                     /locus_tag="PLT86_10550"
     CDS             589..1389
                     /locus_tag="PLT86_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOT37091.1"
                     /translation="MDALLDGSYADLALSCLVLVVFAFVAWRLETVTAPGAVVMTGMG
                     VVILYVGGWHWLLPIVAFFGTSVGLSRIRFGDAEPHGPRDAWQVLANGGVATVGVLLA
                     VMNLPEWRLVDSLAFYAGAIAAATADTWATEIGIRFGGPPRDVINGNSLRKGESGGIT
                     GLGTLAAALGAMMIGLIAVTIPPDQPEDWVALGHVTLAGWLGAMLDSFLGSALQTHYR
                     CRICGAETESPEHCDTATAPRRGFLSNNQVNWACTAFGAFAAWAFVRW"
     gene            1368..2447
                     /locus_tag="PLT86_10555"
     CDS             1368..2447
                     /locus_tag="PLT86_10555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT37092.1"
                     /translation="MGVRQMVRATGLERLYSVCSRAVADGEVPGGVLIVGRHEETLVE
                     LAFGNRAILPLEEPATPDTIYDWASLTKPVLTATLFMQALEEGRVHLWEPLGRWLAVP
                     AGNPLCGVTPRQLLLHISGLPAGLPLPDGGCTRGEYLNLILETGLQNAPDAEFVYSDL
                     GFHLLAAVVEKVYEESLETLAQDRILRPLGMTDTSFCVASGDTSRTAPTERANGEMLR
                     GTVHDPVARAFGGVAGHAGLFGTARDLARFCRMVIGRGQLEGSRILQSATLDRMVAPV
                     DVGSGNARALGWDVDTRYSNPRGDIFPVRGVGHTGFTGPSCWIDPPSGIFIVLMTNRL
                     HPDGHGDAVGLRRRVANVVAAALLP"
     gene            complement(2439..3602)
                     /locus_tag="PLT86_10560"
     CDS             complement(2439..3602)
                     /locus_tag="PLT86_10560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37093.1"
                     /translation="MPKQYQWLSTVKFQDPRTLTYLGSPSILRLPDGALLATHDYFGR
                     GCPLNHVGEEHLTSVYRSEDDGHSWWNVTHISGAFWSGLFLHRGAVYLLGTSAQYGDI
                     VIRRSDDGGFTWTHPKDDSSGLLFRGGHYHDPPNYHCAPVPVLFSSGRIYRAFEDCDP
                     LIWGKGFRAFVISADEDADLLDARTWRMTNKLPYDPEAYPPDFGAIGRDAYGWLEGNM
                     VEAPDGTLWDILRFNSVPVLNKAAMVRVVDEGTRVEFDPETGFIDLPGGLSKFTIRRD
                     PVGGRYWMLSNDMQDFPVPIRRNRLSLFSSADLRAWEKHATLMVDDHEKTPEDSVRNT
                     GFQYVDWQFDGDDIIYLVRTAYDGAHNFHDSNRIVCAKVEGFRKLVGEREVSG"
     gene            complement(3660..4028)
                     /gene="ndhC"
                     /locus_tag="PLT86_10565"
     CDS             complement(3660..4028)
                     /gene="ndhC"
                     /locus_tag="PLT86_10565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT37094.1"
                     /translation="MYPSYIPLFYMLILALVAAGSMLVGVHILGPRRHTYVKNSIPYE
                     SGLDPVAEGRMRFDVKFYLVAILFVVFDLEVVYFYPWAMVFRTLLNEGMTAFYAMLVF
                     TLTLVVGLVYEWKKGALDWK"
     gene            4645..5181
                     /locus_tag="PLT86_10570"
     CDS             4645..5181
                     /locus_tag="PLT86_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOT37095.1"
                     /translation="MKEGFLVTSVKDVIRWGQKNSLWPMSFGTACCGIEIMALLGPRF
                     DLARFGAEASRFTPRQSDLLIVAGRVSIKMMPVLQRIYEQMPEPEWVISMGACASSGG
                     VFNTYTLVQGVDQFIPVDVYVPGCPPRPEALIDAVMEIQQIIDQGDVTIAQFKAFAKT
                     RNPAPGQPLLQVERGTRG"
     gene            5178..5681
                     /locus_tag="PLT86_10575"
     CDS             5178..5681
                     /locus_tag="PLT86_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT37096.1"
                     /translation="MNETPALALQSEFSDIVRGVARQSGAREQWRVDVSKDGVLPVLT
                     YAKTRLDYCYLADITAVDYLSMPNPPEHRFAVVYVLRSRNVSEPLVIRALLPENDAVV
                     ESATSLWNAADWLEREVFDLFGITFLHHPNLIRILMPENFDAHPLRKDFPTEGVGYRD
                     DFPIVTR"
     gene            5745..7037
                     /gene="nuoD"
                     /locus_tag="PLT86_10580"
     CDS             5745..7037
                     /gene="nuoD"
                     /locus_tag="PLT86_10580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOT37097.1"
                     /translation="MKPHTGRPLKFASVPYDPEAPRPPVMPDPIMSRVSKPDPDSGRT
                     IFNFGPSHPSTHGTLRLVLEVEGEMITRAWAEIGYLHTGFEKLAETLNYNQFIPLSDR
                     MNYLSAINNNVGYAHAVEELLGIEITERCKYVRTALAEMSRIADHLVCIGMQAVDIGA
                     YAPFLWGFARREDLYDLFESFCGARLTTSVTRVGGMLRDVPEDFVPGVRAFLSKLEET
                     LRETDRLLTRNRIWMDRTRGIGVLSKEDALRYCVTGPVLRGSGIDHDIRKVQPYFAYD
                     RIDFDVPIGSHGDVYDRYLVRLEEMWQSIRIIRQVLDDLPGGPINVYDNKRVLPPKEE
                     VFTTIEGMIHHFELTMPKFGFKTEVGGQIYSCTESPCGELGFYVVSDGSGNPYRLRVR
                     PPSFVNYAPIEHLVKGATISDVVAIIGSLNIIVGELDR"
     gene            7072..7551
                     /gene="nuoE"
                     /locus_tag="PLT86_10585"
     CDS             7072..7551
                     /gene="nuoE"
                     /locus_tag="PLT86_10585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT37098.1"
                     /translation="MSVQISQTTQEKIAELLTRYPVKQGALLPILHLVQDELGALSLE
                     ALDWVARQIEVSPATVYGVATFYPMFRMEPVGQHHIKVCASLPCALAGCRELIAALKE
                     KLGIEVGQTTPDGKYTLSKTECLAACTEAPAVLVNKKLHRKVTAENASEFLAGFNER"
BASE COUNT         1567 a         2328 c         2384 g         1601 t
ORIGIN      
        1 tcgaaacgcc gtttcgtggg cggtcatcct ctttgccgcg ggtgtttcgc tctcgtggtt
       61 gttgccgatt cgcttcgctc tcatggccac ggtgatcgct ctggcgctcg tgtggtacgc
      121 cgggaaactc aaacgcacga ttctgtgggg ccatattctg gtcgcgatta tcagtggcag
      181 cgcatttgtg ttcggaggaa tgctggggac ccgcctcgct ccagcgttcg cgccggcctt
      241 tcttgcggcg gcgtttcatc tcgggcggga gatgctgaaa gcggcggccg attacccggg
      301 cgatatggaa caggggtacg acacgctcgc cgttcgccgg ggcgttgaac gcacgtgccg
      361 gctggcttcc atcccgcttg caggcgtgat cgcgctctcc ccgataccgt ttattatcgg
      421 ctggtttggc ttttgttacc ttgtcagcgt aattatcctg atagacgggt tgcttgcgta
      481 cgtgatcgca cggatatggc aatcgccttc gagcgaaaca gccgcgcgca tggcgacatt
      541 gttgaaatgg ggcatggtgg cgggcatcgc aaccgtgtgg ggggatgtat ggacgcgctc
      601 cttgacggga gttatgctga ccttgcgttg agttgtcttg ttctggtcgt tttcgcattt
      661 gtggcatgga ggctggagac ggtcaccgcg cccggcgccg tcgtgatgac aggtatgggc
      721 gtcgtaattc tctatgtcgg gggatggcac tggctgctgc ctatcgtggc gtttttcggc
      781 acatcggtgg gcctctcgcg gatacggttc ggcgacgcag aacctcacgg cccgagggac
      841 gcgtggcaag ttctcgccaa cggcggcgtt gcgacggtgg gcgtgcttct cgccgtcatg
      901 aacctcccgg aatggcgcct tgttgattct ctcgcgtttt acgcgggagc aatagccgca
      961 gctaccgccg acacctgggc taccgagatc ggtatccgct tcggaggtcc tccccgggac
     1021 gtgatcaacg ggaattcgct gcgcaagggc gaatctggag gtatcacggg actcgggacc
     1081 ttggcggcag cccttggcgc aatgatgata ggtctcattg cggtcacaat acccccggat
     1141 cagccggagg actgggtcgc tctcgggcac gtgacgctcg ccgggtggct gggtgccatg
     1201 ctggacagtt ttctcggctc ggcgcttcag acacactacc gctgcaggat atgcggcgcg
     1261 gaaaccgaat cgccggagca ctgcgatacg gcgaccgcac ccaggcgagg attcctcagc
     1321 aacaaccagg tgaactgggc ctgcaccgcg ttcggggcat ttgccgcgtg ggcgttcgtc
     1381 agatggtgag ggcaacgggc ctggaacgcc tgtattccgt gtgcagccgc gccgtcgccg
     1441 atggagaagt gcccggggga gtccttattg tcggccgaca cgaggaaaca cttgtcgaac
     1501 ttgcgttcgg aaaccgggcg atccttccgc tcgaagaacc tgctacccct gatacaatct
     1561 acgactgggc ctcgctgaca aagcctgtat taaccgccac cctgttcatg caggcgctcg
     1621 aagaggggcg cgtccacctg tgggaacctc tcgggcgctg gctggcggtt cctgcgggta
     1681 atccactttg cggcgtgaca ccacgccagt tgctgcttca cataagtggc cttcccgcag
     1741 ggctcccgct tccagacgga gggtgcaccc ggggtgaata cctgaacctg atcctcgaaa
     1801 ccggtttgca aaacgcgccc gacgcggagt tcgtgtacag tgatctgggg ttccatctcc
     1861 tcgccgcggt agtggaaaag gtgtacgagg agtcgctgga aacgctcgct caggaccgta
     1921 tcctccgccc gctcggaatg acggacacct cgttctgcgt cgcgtcagga gacacctccc
     1981 gcacagcgcc taccgaacga gcgaacggtg agatgttgag aggcaccgtg cacgaccccg
     2041 ttgcgcgcgc attcggaggc gtagccggtc acgcgggcct tttcggcacc gcacgtgatc
     2101 tcgcacgctt ctgccgtatg gtaatcggac ggggacaact ggaggggagc cgaatactcc
     2161 aatcggccac gcttgaccgc atggtggcac ccgttgacgt tggaagcggt aatgcgcggg
     2221 cactcgggtg ggatgtcgac acgcgttatt cgaatccgcg gggggacatc tttccggtgc
     2281 gcggagtggg gcacaccggg ttcaccgggc cctcctgctg gatcgatccg ccgtcgggga
     2341 tcttcatcgt cctgatgaca aaccgcctcc atccggacgg acacggggac gcggtggggc
     2401 ttcgcagacg cgtggccaat gtcgtcgcgg cagccctgct accctgaaac ctccctctct
     2461 cccaccagtt tcctgaaccc ttccactttc gcacacacaa tccggttgga atcgtgaaag
     2521 ttatgcgcgc cgtcatatgc cgtgcggaca aggtagatga tgtcgtcccc gtcaaactgc
     2581 cagtccacgt actgaaagcc ggtgttcctc actgaatctt ccggcgtctt ttcatggtca
     2641 tccaccatca gggtagcatg tttttcccac gccctcagat cggcggagga aaacagcgag
     2701 agacggttgc gccggatcgg caccgggaaa tcctgcatat cgttggagag catccagtaa
     2761 cggccgccga caggatcccg ccggatggta aacttggaga ggccacccgg caggtcaatg
     2821 aacccggttt ccggatcgaa ttccacacgt gtgccttcgt caactacccg gaccatcgca
     2881 gccttgttga gcactggaac agaattgaac cggaggatgt cccacagcgt cccgtcgggc
     2941 gcttcgacca tattcccttc cagccacccg taggcgtccc ggccgatcgc gccaaaatcg
     3001 ggcggatagg cctcgggatc gtacggcagc ttgttcgtca tcctccatgt gcgggcgtcc
     3061 agaaggtccg cgtcctcatc ggcggagatc acgaacgcgc gaaacccttt gccccagatc
     3121 aacgggtcgc aatcctcgaa cgctcgatag attctaccgg atgaaaacag cacgggaacc
     3181 ggcgcacaat ggtagttcgg cggatcgtga taatgcccgc cacggaacaa caggccgctc
     3241 gaatcatcct tcggatgcgt ccaggtgaac ccgccgtcgt cagaccgccg tatcacgata
     3301 tccccgtact gcgcggaggt gccaaggaga tacacggcgc cgcggtgaag aaaaagaccg
     3361 ctccagaacg cacccgaaat gtgggtgacg ttccaccacg agtgaccgtc atcctccgaa
     3421 cggtagacgc ttgtgaggtg ttcctcgccc acatgattca aaggacaccc gcgaccgaaa
     3481 tagtcgtgcg tggcaaggag tgccccgtcc ggaagccgga ggatgctcgg gcttccgagg
     3541 taggtcaagg tgcgcgggtc ctggaatttt acggtgctga gccactggta ctgtttcggc
     3601 atcgggaggt tcacctcagg ggaggctgtt tcacaaccgg agtttccgtt ccgggggact
     3661 catttccagt cgagcgcgcc ttttttccat tcgtacacga gcccgactac cagcgtgagc
     3721 gtgaaaacga gcatcgcgta gaacgccgtc atgccttcgt tcaacaacgt tcggaacacc
     3781 atcgcccaag gatagaagta gaccacctca agatcgaaca ccacgaaaag gatggcaacg
     3841 agataaaact tcacgtcgaa gcgcatccgc ccttccgcca cagggtccag acccgattcg
     3901 taagggatcg agttcttgac ataggtatgg cgccgcggac ccagtatgtg cacgccgacc
     3961 agcatggacc cggccgccac aagcgcaagg ataagcatat aaaacaaagg gatatacgac
     4021 ggatacatga tacctccgtg ccacgtctgt tcggtggtga tatgcgtaac ctgcatggga
     4081 aaatagtctc actctgcagg ttttgcaagc tgtgacccgg gatggggaaa agggaagggc
     4141 gcaggcgtct gccggacacc tgcgccctcc aaggggatcc cggtcagcca agggggagga
     4201 agccgaccgg gataaggtgt gatagccggg aggaggaccg tccatcacac ggggatcatt
     4261 attctcccaa tctattcagt attaacacct cgagcggcac gcgggttccc ttcggagccc
     4321 ctcttggact cgccgtatta actggtaaaa gtacgcaatg tctgtgccag ttctggaacg
     4381 tcgaacagct ggttccgtcc tgttgctaaa cgtttaatat tgtgcattaa tctggttttg
     4441 gcaagcccct ttccacaaaa ttcttcacgg atattcgcgg ccgaattcgg gaccgagaag
     4501 cggcgcattt tcgggcgcag ccagatggat ccttgtccgc gcgcaagctg gctcctatct
     4561 ttggtttgca acagccgggg cggggttttc cggcgtcctg caaaatccac cgttgtcgaa
     4621 cgcgggccat tgcgaggttt cgccttgaaa gaaggttttc tcgtaacgag cgtcaaagac
     4681 gtcatccggt ggggccagaa aaacagcctc tggccgatgt catttggcac ggcgtgttgt
     4741 ggaatcgaaa tcatggctct gctcggcccg cggtttgatc tcgcacggtt cggtgccgaa
     4801 gcatctcgct tcactccccg gcaatcggac ctgctcatcg tcgccggacg cgtctccatc
     4861 aagatgatgc cggtgcttca gcggatctac gagcagatgc ccgagccgga gtgggtaatc
     4921 agcatgggcg cctgcgcatc gtccggggga gtattcaata cgtacaccct ggtccaggga
     4981 gtggaccagt tcattccggt ggacgtgtac gtgcccggtt gccccccgcg ccccgaagcc
     5041 cttatcgatg cggtcatgga gattcagcag atcatcgacc agggcgacgt tacgattgcc
     5101 cagttcaaag cgttcgcgaa aacccgcaat ccggctcccg gacagccgct gctgcaggtc
     5161 gaacgaggaa cgcgcggatg aacgagacac ctgccctcgc cctgcaatcg gagttctcgg
     5221 acatcgttcg cggtgttgca agacaatcgg gcgcgcgtga gcaatggcgc gttgacgttt
     5281 cgaaagacgg cgttctgccg gttctcacgt acgcgaaaac cagactcgat tattgctacc
     5341 ttgccgacat caccgcggta gactacctct cgatgccgaa tccgccggag caccggttcg
     5401 cagtggttta cgtcctccgc tcgcgcaatg tgtccgagcc gctggtgatc cgggcactct
     5461 tgccggaaaa tgatgcggtg gtggagtccg caacctcgct gtggaacgca gcagactggc
     5521 tcgagcgcga agtgtttgac ctttttggaa tcacgttcct tcatcacccg aacctgattc
     5581 gaattctgat gccggaaaac ttcgacgcgc acccgctgcg gaaggacttt cccacggaag
     5641 gcgtcgggta tcgggacgat ttcccgatcg ttacgagata gaccgatcga cacgcgcggg
     5701 cgcattccgg ccccgtcggt tcgatttgtg cgcaggaaca cgtcgtgaaa ccacacaccg
     5761 gcagacctct caagttcgcg tccgtcccgt atgatccgga ggccccgcgc cctccagtca
     5821 tgccggatcc gatcatgagt cgagtctcga agccggaccc tgattccggg cgcacgattt
     5881 tcaacttcgg tccttcccat ccttccacgc acggcacgtt gcggctcgtg cttgaggtgg
     5941 aaggcgaaat gatcacgcgt gcatgggcgg aaatcggtta tctgcacacc gggttcgaga
     6001 agctggcgga aacgcttaac tacaaccagt tcattccgct cagcgaccgg atgaattatc
     6061 tctccgcgat caacaacaac gtcgggtacg cccacgcggt tgaggagctg ctgggaatcg
     6121 agatcaccga gcgatgcaag tatgttcgca ccgccctcgc agagatgagc cgcatcgccg
     6181 accacctggt gtgcatcggg atgcaggcgg tggatatcgg ggcgtacgct ccatttctct
     6241 gggggtttgc tcggcgcgag gatctgtacg acctgttcga gagcttctgc ggggcacggt
     6301 tgaccacctc tgtgacccgc gtcggtggga tgttgcgcga tgttccggaa gatttcgtgc
     6361 cgggagtccg cgcatttctg agcaaactcg aagaaacgtt gcgcgaaacc gacaggcttc
     6421 ttacccgcaa ccggatctgg atggatcgca cccggggaat cggtgttctt tccaaggaag
     6481 acgccctgcg ttactgcgta accggtccag tgctccgggg aagcgggatt gaccatgaca
     6541 tccggaaggt gcaaccgtat ttcgcgtacg accggattga tttcgacgtg ccgataggct
     6601 cgcacggcga tgtgtacgac agatacctcg tacgcctcga ggagatgtgg cagagcatac
     6661 gaatcatacg acaggtactt gatgacctgc ccggcgggcc aatcaacgtg tacgacaaca
     6721 agcgcgtgct tcccccgaag gaggaagtgt tcaccacgat agaaggaatg atccaccact
     6781 tcgagctcac catgccgaag tttggcttca agacggaggt cggcgggcag atctactcgt
     6841 gcaccgaatc tccctgcggt gagctgggct tttacgtggt gtcggatggt tcggggaacc
     6901 cgtaccgtct gcgtgttcgc cccccatcgt ttgtgaacta cgccccgatc gagcacctcg
     6961 tgaaaggcgc gaccatttcg gacgtggtgg ccatcatcgg cagcctgaac atcatcgtcg
     7021 gggaacttga ccggtaaatc ggcgcgcgaa agattacgcg ggaatatctc aatgtcagtg
     7081 cagatcagcc agacgacaca ggagaagatc gcggaactgc tgacccgata tccggtgaag
     7141 cagggcgcgc ttctgccgat ccttcacctc gtgcaggacg aactcggagc tctttcgctc
     7201 gaggccttgg actgggtagc ccggcagatc gaagtctcgc ccgcaaccgt gtacggcgtg
     7261 gcgacctttt acccgatgtt ccgcatggaa ccggtcgggc agcatcacat caaggtatgt
     7321 gcgagtctgc cctgcgcgct ggcaggatgc cgcgaactca tcgcagcact caaggagaag
     7381 ctcggaattg aggtggggca gacgacaccc gacgggaagt acaccctctc caaaacagaa
     7441 tgcctcgcgg cctgcacgga agccccggcc gtgctcgtga acaagaaact gcaccgaaag
     7501 gtgacggcgg aaaacgcctc tgaatttctc gcaggattca acgagagata gcctgccttc
     7561 gtgtcgcaga tgacgtgcac cacttccgcc gcgaatgcct cctaagcaac aatacgcgcg
     7621 ggccctgctg aagacccact gaaactccac tctgcatccg tcccgcctcg gttgcgtctg
     7681 gtggttctgc tccggaaatt cctatgcagt gctctgcggg ccttttttca ccaccaacgg
     7741 ggatcccaca atgagatgga tcggggtttt gctcatggcg gcgacagttt tcgccggatg
     7801 cgcggcgcga acggatagtt cgcgagcgcg gacaaacgct tcgcgagcgc ccaactggat
     7861 cagtgaagtg agataccagg
//
LOCUS       DAOSOO010000156         7800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9320_length_7800_cov_5.728986, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000156 DAOSOO010000000
VERSION     DAOSOO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_7800_cov_5.728986"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            589..1899
                     /locus_tag="PLT86_10590"
     CDS             589..1899
                     /locus_tag="PLT86_10590"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37099.1"
                     /translation="MDPRSRRSILGWALYDFAESAFATSILVAILPVYYLSIAPQGGI
                     AVAFGPIRFTTAASSLWAYTISFSTMLIALPSPVLGALADSGGRRKLFLGVFGYTGAL
                     FSALLVFVGTGDYLLASALFCVANIGSVGSSTFLNALLLDVAPPGKMDSVSGKGFAAG
                     YLGGGTVFVINLLMIAHPEWFGIPSQEWGIRLSFLVVGLWWALFSIPTMLWVRERPRE
                     EKTHGSRIVREAFRTTWMTLRRITRFRDLSLYLLAFLVFNDGIQTVIVMAVPYAKETL
                     RLDTTTLMGVLVLIQAIGIPGSLLFGWVAERLGSKRALLISLTIWSGVVIMAWRMTSA
                     VEFWVLGACVGLVLGGSQAISRSLYGALIPQARAAEFYGFLAVSSRFSSFLGPLVFGL
                     ARDITGSMRAGILALIVFFIVGSGLLLLVNMQRGIERARAEEAG"
     gene            complement(2062..3273)
                     /locus_tag="PLT86_10595"
     CDS             complement(2062..3273)
                     /locus_tag="PLT86_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT37100.1"
                     /translation="MTAPANTDRLVWWKEARFGMFIHFGVYSLVSHGEWIYYQEHIPQ
                     EEYNRLADQFRPTHYNPREWVRIAQDAGMRYMVMVTRHHDGFCMYDSKVSDFTSTKRA
                     AKRDFIAEFADACHEAGMRMGLYYSLVDWRFPGALFNIPRQADEVYAPMVEQAHAQVR
                     EILTNYGKVDILWYDMAVPAGVWRPEELNAMARELQPGILINNRAGTPEDFGTPENAI
                     VPEKRAWEACYTMNETWGYAEGDRNWKSANQLIYLLATCASGGGNLLLNVGPEPDGRF
                     PREAVDRLRQVGAWMRTNGAAIYGAQPSPVTAPALGWGTYAGGKAYLIILRWPGSTVP
                     FAWCANRVLKAKLLITGEEARIEQKGDRVWLHGFPEYPPDNLPSVVELTLEGEPQAAV
                     PSLAFGNKLEV"
     gene            complement(3357..4430)
                     /locus_tag="PLT86_10600"
     CDS             complement(3357..4430)
                     /locus_tag="PLT86_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37101.1"
                     /translation="MTGRERLRAILRKQPKDRLAWTTLVDNNTLALLPEHLRGNGGID
                     FYKHLGCDIFLLNGWNTPHDLRAPVHQWPESVAVSYEQEGARVTTRWRTPHGELTGIT
                     QNGHPIKYPVDSIDAVRIYTAMWEGVTFHPYHDHPALAALDELIGEYGVVTRFWGPST
                     IPRLLEQDMGTQHFYYLLADHPGDMRALVRTMHERELEAFKLLAEGPWESVTLVENTS
                     TFYISPAVYREFNMPHQRDFVETVKATGKTALLHMCGHVHRILDLIRQTGCDGIHALT
                     PPPTGDTPWEAALDVLGEDTIIFGVLDPTIFVAGDVEEIAPSLDRLITPRLRKANFVL
                     CPFADGIPVPMERFLAVKEWAGR"
     gene            complement(4453..>5817)
                     /locus_tag="PLT86_10605"
     CDS             complement(4453..>5817)
                     /locus_tag="PLT86_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37102.1"
                     /translation="DTVMVRTTVGEVPGGLVITPHKVTQVPTNPDSGKVVWDPIIPLG
                     EVESVAIPRLETREFWFDVDAAKATAGEHTVKVTFSAGGKETVVEIALRVLPFEMGGY
                     DAMRLCMWASYNKHTVPDLLAHGATVFLTGLPPAKAVEQGGQTRLEVDFTAMDQFLAP
                     LEGHDVFLLLTGLPQVGVGEDDPSYVARLADYFEQVMSHLAAKGIPEEHVALYTWDEV
                     GGNGWDAVRRYVAFGKLVLKARPTIKFYVNGGGDLPMFEELVEIAAIWSPAFFMLDDD
                     TPVMRYLWNTKKTMWTYNCAYMYARPMGWNTKAINIVGEFRMQAVFAVRYGATGIGYW
                     CYNHGESMWNPVHAEYPIVYQTSPDGPVTSSRRWEAVRESVEDSRIVVALREKLADAS
                     VSDAAKEKIRHLVEVTLPGMADKSLTEMHLGTAPYVLDDTSNDAMVAAFREELLDCVE
                     LVSG"
     assembly_gap    5818..5917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5918..>7800)
                     /locus_tag="PLT86_10610"
     CDS             complement(<5918..>7800)
                     /locus_tag="PLT86_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT37103.1"
                     /translation="RTGKEMWRVPTNQRYMTCPVVLERPGDSALIYAADMSWSTKPES
                     TLSCIDGAGKIVWQAKVASVYWASPALADINGDGRVELVQGDAAGIVHAYDALTGALV
                     WEHQLDGECSSPGIGDLDGDGAPEIAISTTTGRLYVLNGAGEIVREKLLGGTVFNPVG
                     EYAGYRVCSPVLFANSRGEARMIASVQEPGSRRILCFNGNLDLLWERETPGGIGPSVS
                     VADFDGDGRADIFAPAQSGRLFRFDEDGRVVWDFDTQAFCYAPGAIVDVDGDGALEYV
                     LCTELGKILVYNQAGEIVFTHQCDSRRAHRTTPAFGKIADGPRGLVFGITGSDTGRLY
                     CFTVPASADTHVEWHAFRGSPRSTGSWFGLQKVEAVRMEPENLVWDAVLTQDEITFRV
                     TNPAPGKEPLEAEATVLAPDGVRQGATGIIAGEKGVLKMPVAISAPGKYRFEWCARKA
                     DGEVLARGSRALTLSPYRNDQALARKAVVALRDAVAGVPTDAGFPAVLREECAAIEQE
                     AGNLAVLQLAGAASAFSPEVVARTGKLNARAKRALALAGIAPTVLSDASGCPVLPFEG
                     RMWENLNVDAELPGRLFLPLAIRRRCVTGEHEPVSIKLLNVSPDTVMVRTTVGEVPGG
                     LVI"
BASE COUNT         1260 a         2504 c         2415 g         1521 t
ORIGIN      
        1 tggaacacgc ccggccggtg gtgcgcaccc tttccgggcg acgcgtgcaa gcatctcgaa
       61 ccgtttgtgg cggagcggct gccggtgggt gaatctgcat ccatcaatgg ccggatcggc
      121 atgcatgagg ggtaccgggc gtcgctggcg gggttgttcg ggggcttggg ggcattcgtg
      181 gcgctctctg acagagcaca ccacctttca gaagtgcgta caccgcccgg tcctgcgaac
      241 cgggctgggc tgtttcgcgc cgttggcgct gggcgcccgc tgttgcgggc gaaaggaagg
      301 gctgaaaggc cgcaataatc cagcccggct catcgggccg ggcgcccgtg ccgagccggg
      361 agcggcggct tgttgggagg ccgccgcgga cagtgtggag ggcaggcagg aatgcctgcc
      421 ccaccgatat ttgttgcgac cggccgcacg gtgggacagg cattttgcct gtccagtgcc
      481 tcctccctcc tccagtggtt gccttcgtcc gttctcccgt gtttcgtatc cttttcgcag
      541 gacttcggct gttccgcttc ttctgcccgc acatgaggag gcattcgcgt ggatccccgt
      601 tcgcgtcgct ccatcctcgg gtgggcgctg tatgatttcg cggaatcggc gttcgccacc
      661 tccatcctcg ttgccatcct gcccgtctac tacctctcca tcgctccgca gggcggcatc
      721 gcggtggcgt tcggcccgat caggttcacc accgcggcat ccagcctctg ggcgtatacg
      781 atcagtttct ccaccatgct gatcgccctc ccttcgccgg tgctgggggc gctggcggat
      841 tcgggaggcc gcaggaagct cttcctcggc gttttcgggt acaccggagc gttgttcagc
      901 gcgctgctgg tgttcgtcgg cacgggggac tacctcctcg cgtcggcgct cttctgcgtc
      961 gcgaacattg gctccgtagg cagcagcacc tttctgaacg cgctgctgct ggacgtggcc
     1021 ccacccggca aaatggattc ggtctccggc aaaggctttg cggcaggcta tcttggcggc
     1081 gggacggtgt tcgtcatcaa cctgcttatg attgcgcacc cggagtggtt cggcattccc
     1141 tcgcaggagt ggggcatccg cctgagtttc cttgttgtcg ggctctggtg ggcgctgttc
     1201 agcattccca cgatgctgtg ggtgcgggag agaccgcggg aagaaaagac gcacggctcc
     1261 cgcatcgtcc gcgaggcatt ccgcaccacg tggatgacac tccggcgcat cacacggttc
     1321 cgcgaccttt ccctgtatct ccttgccttc cttgtgttca atgacggcat ccagacggtg
     1381 atcgtgatgg cggtgccgta tgccaaggaa acgctcaggc tcgatacaac cacgctgatg
     1441 ggcgtgctgg tgctgattca ggccatcggt ataccggggt cgctgctgtt cgggtgggtg
     1501 gccgagcggc tgggttccaa gcgggcgctc ctcatcagcc tcaccatctg gtcgggcgtc
     1561 gtcatcatgg cgtggcgcat gaccagcgcc gtggagttct gggtgctggg cgcctgtgtc
     1621 gggctggtgc tgggcggcag tcaggcaatc agccgttcgc tgtacggcgc gctgattccc
     1681 caggcgcgcg cggcggagtt ctacggattc ctcgcggtca gcagccgatt ttcgtcattc
     1741 ctcggtccgc tcgtcttcgg tctcgcgcgc gacatcaccg gcagcatgcg agcgggcatc
     1801 cttgcgttga ttgtgttctt catcgtgggc agtgggctgc tgcttctcgt caacatgcaa
     1861 cgcgggattg aacgcgcccg ggcggaggaa gccggatagg acgtttcgtt acggtgttct
     1921 ttttctggat tcccgccttc gcgggaatga cggcacggtt ggcgggggag cggcacgaat
     1981 acctcgctgc ccggaagggg ccttggatga caacttacca tcctccggca ggccatggtg
     2041 gggcaggcag gctgccctgt ttcacacttc cagcttgttc ccgaacgcca gcgatggcac
     2101 ggctgcctgc ggttcgccct ccagcgtcaa ctcaaccacc gaaggcaggt tgtcgggcgg
     2161 gtactccggg aacccgtgca gccagacgcg gtcgcccttc tgctcgatac gggcctcctc
     2221 gccggtgatg agcagtttcg ccttcagcac cctgttcgcg caccacgcga acggaaccgt
     2281 tgaccccggc cagcggagga tgatcagata cgccttgccc ccggcgtacg ttccccaacc
     2341 gagcgccggc gcggtgacgg gcgacggctg cgcgccgtag atcgctgcgc cattggtgcg
     2401 catccatgcg cccacctgtc tcagtcggtc caccgcctcg cgtgggaaac gcccgtccgg
     2461 ttccgggccg acattcagca acaggttgcc gccgccggac gcgcatgtcg cgaggaggta
     2521 aatcagctgg tttgctgatt tccagttccg gtcgccctcc gcataccccc atgtctcatt
     2581 catcgtatag cacgcttccc atgcgcgttt ctccggcacg atggcatttt ccggcgtgcc
     2641 aaaatcctcc ggcgtgccgg cgcggttgtt gatcaggata ccgggctgaa gctcccgcgc
     2701 catggcgttg agttcttcgg gacgccacac accggcaggc accgccatat cgtaccaaag
     2761 gatgtccact tttccgtagt tggtcaggat ctcgcgcacc tgcgcatgcg cctgctccac
     2821 catcggggcg tacacctcat ccgcctgccg cgggatgttg aacagcgcgc cggggaagcg
     2881 ccagtccacg agggagtagt agagccccat gcgcattccg gcttcgtggc aggcgtccgc
     2941 gaattcggcg atgaagtctc tcttcgccgc gcgtttcgtc gaggtgaaat cggacacctt
     3001 gctgtcatac atgcagaacc cgtcgtggtg gcgcgtcacc atcaccatgt agcgcatgcc
     3061 ggcgtcctgc gcgatgcgca cccattcccg tggattgtaa tgtgtggggc ggaactggtc
     3121 ggcaagcctg ttgtattcct cctgcggtat atgttcctgg taataaatcc attcgccgtg
     3181 gctgacaagg gaatacacgc cgaagtggat gaacatgccg aaacgtgctt ctttccacca
     3241 tacaaggcgg tcggtgttgg caggggctgt cattgggatc tcctgtgagt tcgatagggt
     3301 ctgttcgcaa aaaaaccatt cggtagtgct cgccattcga cgactggcgc tgtgcgttat
     3361 cgccccgccc actccttcac tgcgaggaac cgttccatcg gaaccgggat accatcggcg
     3421 aacgggcaca gcacgaagtt cgccttccgg agccgtggag tgatgagccg atcgagcgat
     3481 ggcgcgattt cttcaacgtc cccggcaacg aaaatggtgg gatcgagtac tccaaaaatg
     3541 atggtgtctt cccccagcac gtcaagcgcg gcctcccagg gcgtgtcgcc cgtcggcggc
     3601 ggggtgaggg cgtggattcc gtcgcatccg gtttgcctga tcagatcgag tatccggtgc
     3661 acgtgcccgc acatgtggag cagcgcggtt ttcccggtcg ccttcacggt ctccacgaaa
     3721 tcccgctggt gcggcatgtt gaactcgcgg tacaccgccg ggctgatgta gaaggtgctc
     3781 gtgttttcga ccagggtgac cgactcccac ggcccttcag cgagcaactt gaaggcttcc
     3841 agttcccgct cgtgcatggt cctgacgagc gcgcgcatgt cgcccggatg gtccgccagc
     3901 aggtaataga agtgctgcgt gcccatgtcc tgttcgagca gccgcggaat cgtggacggg
     3961 ccccagaagc gggtgacaac accgtattcg ccgatcagtt catccagagc cgccagcgcc
     4021 ggatggtcgt ggtacgggtg aaacgtgacg ccttcccaca tcgcggtgta gatgcgcacc
     4081 gcatcaatgg aatccaccgg atacttgatc gggtgcccgt tttgcgtgat ccctgtgagt
     4141 tcgccgtgag gcgttctcca ccgggtggtg actctggcgc cctcctgctc gtacgacacg
     4201 gccacgctct ccggccactg gtgcaccgga gcgcgcaggt cgtgcggcgt gttccagccg
     4261 ttcagaagga agatatcgca cccgaggtgc ttgtagaaat caatcccacc gttgccgcgc
     4321 aggtgctccg gcaggagggc gagggtgttg ttgtcgacca gggtggtcca cgcaaggcga
     4381 tccttcggtt gtttccgaag tatcgcgcgc agccgttcgc gtcccgtcac acggcatccc
     4441 cgtcctgagg gttcaacccg aaacaagttc gacgcaatcg agcagctctt cgcggaatgc
     4501 ggcaaccatg gcgtcgttgc tcgtgtcgtc cagcacatac ggcgcggtgc cgagatgcat
     4561 ctccgtgagc gatttatccg ccatgcccgg cagcgtcacc tcgaccaggt ggcggatttt
     4621 ctctttcgcc gcatcgctga cggaagcatc ggcgagcttt tcgcgcaggg cgacgacgat
     4681 acgcgaatcc tccacgctct ccctgacggc ttcccagcgg cgcgaggagg ttaccggtcc
     4741 gtcggggctg gtctggtaaa caatcgggta ttcagcgtgg acggggttcc acatggattc
     4801 gccatggttg tagcaccagt agccgatgcc ggtcgcgccg tagcgcacgg cgaataccgc
     4861 ctgcatgcgg aactctccca cgatgttgat cgccttggtg ttccatccca tggggcgggc
     4921 atacatgtag gcgcagttgt acgtccacat ggtcttcttt gtgttccaca gataccgcat
     4981 cacaggtgtg tcgtcatcga gcatgaagaa cgcgggcgac cagattgcgg cgatttccac
     5041 cagttcctcg aacatcggca gatcgccgcc gccgttcacg taaaacttga tcgtcggccg
     5101 cgccttcagc accagcttgc cgaaggccac gtagcgccgt acggcatccc acccgttgcc
     5161 gccgacttcg tcccaggtgt agagcgcgac atgctcctcg ggaatgccct tcgcggcgag
     5221 gtgcgacatg acctgctcga agtaatccgc aaggcgggca acgtaggagg gatcatcctc
     5281 gccgacgccg acctgcggta accccgtcag cagaaggaac acatcgtgcc cttccagcgg
     5341 cgcgaggaac tggtccatgg cggtgaaatc aacctccagg cgcgtttgtc cgccctgttc
     5401 cactgccttc gcaggcggca ggccggtcag aaaaaccgtt gcgccgtgcg ccagcaggtc
     5461 ggggacggtg tgcttgttgt acgacgccca catgcagagt cgcatcgcat cgtagccgcc
     5521 catctcgaat ggcagcaccc gcagcgcgat ttcgaccacc gtttccttcc cgccggcgga
     5581 gaacgtcacc ttcacggtgt gctcgcccgc ggtggccttc gcggcgtcca catcgaacca
     5641 gaactcccgc gtttcgaggc ggggtatggc aaccgattcc acctctccaa gcggaatgat
     5701 ggggtcccac accactttcc cggagtcggg gtttgtgggc acctgcgtca ccttgtgcgg
     5761 cgtgatgacg aggccgccgg gaacctctcc caccgtggtg cgcaccatta cggtgtcnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga tgacgaggcc gccgggaacc
     5941 tctcccaccg tggtgcgcac cattacggtg tccggggaca cgttgaggag cttgatggac
     6001 accggttcgt gctcgccggt gacacaccgc ctgcggattg caaggggcaa aaacaacctg
     6061 cccgggagct ccgcatccac gttgaggttc tcccacatgc gcccttcgaa gggcagaaca
     6121 gggcacccgg aggcatccga aagaaccgtc ggagcgatcc ctgcaagcgc gagcgcacgc
     6181 ttcgcccggg cgttgagttt gccggtccgc gccaccactt cgggagaaaa cgctgacgcg
     6241 gcgccggcaa gctgcaacac cgcaaggttg ccggcctcct gctcaatcgc ggcacattcc
     6301 tcgcgcagaa ccgccgggaa gcccgcgtcc gtcggtactc ccgcaaccgc atcgcgcagc
     6361 gcgaccaccg ccttccgcgc gagtgcctga tcgttccgat agggcgaaag cgtcagcgcg
     6421 cgcgaacccc gggcaaggac ctctccatcg gctttcctcg cgcaccattc gaaccggtat
     6481 ttccccggcg cactgatggc caccggcatc ttcagcacac ccttttcccc ggcgatgatg
     6541 ccggttgcgc cctgccgcac gccatccggc gcgagcaccg tcgcctcggc ctcaagcggc
     6601 tccttccccg gcgcgggatt ggtgacgcgg aaggtgatct cgtcctgcgt cagcacggcg
     6661 tcccacacga ggttctccgg ttccatgcgc actgcttcga ccttctgcag cccgaaccac
     6721 gatccggtgg aacgcgggct cccgcggaag gcgtgccact cgacgtgcgt gtcggcgctg
     6781 gccggcaccg tgaagcagta aagccgcccc gtgtcgctgc cggtgatgcc gaataccagt
     6841 ccgcgcgggc catcggcgat tttcccgaac gcaggggtgg ttctgtgcgc acgccggctg
     6901 tcgcactggt gcgtgaagac gatttccccg gcctgattgt aaacgaggat ctttccaagt
     6961 tccgtgcaca gcacgtattc cagcgcgccg tcgccatcca cgtccacgat cgcgccggga
     7021 gcgtagcaga aggcctgcgt gtcgaaatcc cacaccacgc ggccgtcctc gtcaaagcgg
     7081 aacaaccttc ccgactgcgc cggggcgaag atatccgcgc ggccgtcgcc atcgaaatcg
     7141 gccacggaaa ccgaggggcc gatgccgccc ggagtttccc gctcccagag caggtcgagg
     7201 ttgccattga agcagagtat ccggcgggag cccggctcct gcaccgacgc aatcatacgg
     7261 gcttcgccgc gtgaatttgc gaacagcacg ggagagcaga cgcggtaccc cgcgtattcg
     7321 cccaccggat tgaacaccgt gccgccgagg agtttttcgc gaacgatctc tcccgcgccg
     7381 ttcagcacgt acaaccggcc ggttgtcgtg gagatggcga tttcgggcgc gccgtcgccg
     7441 tcgaggtcgc cgatgccggg gctggagcat tcgccgtcaa gctggtgctc ccagacaagc
     7501 gcgcccgtga gcgcgtcgta tgcgtgcacg attcccgccg cgtcgccctg cacaagctcc
     7561 acccgtccat cgccatttat atccgccagc gcgggggagg cccagtacac ggaggcaacc
     7621 ttcgcctgcc agacgatttt gccggcgccg tcgatgcagc tcagcgtgga ttcgggtttc
     7681 gtgctccaac tcatgtcggc ggcgtagatg agcgccgaat caccgggccg ttcaagaacc
     7741 acggggcagg tcatgtagcg ctggttcgtg ggaacccgcc acatttcttt gcccgtgcgg
//
LOCUS       DAOSOO010000157         7767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9373_length_7767_cov_5.287733, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000157 DAOSOO010000000
VERSION     DAOSOO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_7767_cov_5.287733"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="aroC"
                     /locus_tag="PLT86_10615"
     CDS             complement(<1..526)
                     /gene="aroC"
                     /locus_tag="PLT86_10615"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT37104.1"
                     /translation="MPGNSFGHSFRITTFGESHGPAVGVVVDGCPPGIPLAEEDIQRE
                     LDRRRPGQSLIASARQETDAAEILSGVFEGVTTGTPIAVLVRNEDARSRDYAAIKDLF
                     RPGHADYTYLAKYGVRDYRGGGRASARETIGRVAAGAIAKKLLGYEGVSVLGYTCQVG
                     DVKMPVMERVTLEQV"
     gene            850..6948
                     /locus_tag="PLT86_10620"
     CDS             850..6948
                     /locus_tag="PLT86_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT37105.1"
                     /translation="MLRAVLRAVLVLAVSLLGPVAHAETTVSGEITTTTWTKANSPYR
                     VAGDLYIPPGNTLTIEQGVDVIFDADALFTVEGMITATGTAADSIRFFPSGDGSWRGF
                     RVYSEGMVAAKPLSERAKAARVTQEGGASIEMAFARISGVQDADDEATGGAFEVVGAA
                     SGLTLTNCVIRDNTAYEGAGIAFVGGAGGMLSLVTFANNSASNQGGAIYLSDAFVQAI
                     SCTFVDNHAVNFGGAIAGIDYDTIGPLYRSAAGRSGQNGDGRGKRPSGAERPARPESP
                     VPRVARRAEDGEPSFIMLVLAQIRNNTSGDCGGALYLQGTVGALMGSTVAQNSTTYDG
                     GGIYATNGFVQMQGCSVDSNSAGQDAGGVALYSQTMAGITDTRFRANQAEGDGGALNL
                     WDGAKLTIETSTFENNRSSDDGGAMTVYTGSTATVRECQFRGNAAGYDGGGIIVWDDC
                     ELTVENTQFEGNSSTSDGGALVVYTGSTATISESTFQGNTCDDGEGGGYGGAFEAWQN
                     VTVTMGSCTFTGNFAPESGGAVGIYDQSALTFRGCDFGGNHTETFGGALDVWYSDLQL
                     VDCALRNNAVTGPGYGGGVYAFNSRIAASNVLVAKNEADYGGGMYFDADASLALDRGT
                     VAHNVGRTGVGGLFLYGPGHSIASSIIWGNTGGNVYVTDQANVTVAYSDLPYEGVWPG
                     TGNINADPMFVDAAGGDFTLAEGSPCINAGDPAGDPDPDGSLPDMGWSPTGGGSIGGT
                     EVSGLLTTGTWTRANSPYRVTGDLELPEGEVLTIEPGVDVIFEEPAFFYVYGRLIAHG
                     TETDSIRFYPASAAAGWHGFEIESYGGMYRPLSTRAKATRAAYEGGASVEMSYVRISG
                     VRDIADYNDGGAFMVYGNTASVSVSNAIIRDNEAYDGGAANFVDGAMGQFTNVMFADN
                     YSEGDGGAVTFNDAFVSFEGCTFARNESYYAGGAVYVYDYDGGGLDKPAARAKADKHG
                     KADRRAVERPSRRADRLSARPARPTRPHRAAREDRSTKVSRDQRRALPGRAARAARPT
                     RQATDGELSYLYVEDCQFVDNYSDEDGGALYLHGVQGEFSGTSFERNEAYWEGGAVYS
                     DYSALSFTLSSFEGNYGPDEGGAVDAGYSTLSFDSCAVKNNVSDGYGGGFSLDGSVYL
                     TVNRSLFEGNSTDEGGGIVAYDDCHMVVQNSAFIGNSAWSYGGAMAVNSSSTLEVSNT
                     SFVDNTSEGTSGGIEVWDYSSASADGCTFTGNVGIDGGGGMGVYDDATVVVSNSTFRN
                     NGSAFSGGGVDVYQAGATIVECVFENCTAQNDADWTYGGALYANDAQLDVGRTLIAKS
                     TADHGGGVCLEMDVTATFTNCTIADNTGVEAGGGISLYDGVVTLNLLNTIVAWNEGGN
                     VLNPPEGLGQVVATYSDIFDEEVWPGERNISADPLFVDREAGDYRLAAGSPCIDAGDP
                     ESERDPDGTIADMGAFPTEKQGGPTPVSGTILTTLWKSTKSPYRVVGAINVPAGNVLT
                     IEAGVDVLFDADVDFIVNGKIVVTGTQTDSVRFLAGATEWGGFSILSPTDTSLFRYAR
                     FSGARANSGQYDGAGAMYINASKAILEHCVVSGNSGTYCGGGMGLDSDAMVWMSDCAV
                     VDNSCGHDGGGIYSARSTLQMQRCVVARNSSGGAGDGIQVGFDSVVDLVNCTVVANGG
                     AAFYWYPAGTLRLKNCIVWGNSSAGLITSATYSDIQGGVGGAGNINADPLFVNAAAGD
                     YRLSPGSPCIDAGDPASPRDPDLTRADMGAFPTGIPSKYGDVTGDGTVSAGDASLVLR
                     YVVGIVPSLPRPVAADVTANGSTSSYDAALILRKVLDDTYLFPAERGGMPGTKMAGTL
                     PRVLSWERAGSGWALRVNDPTGILAGDFQFALPDAREVNVAAGEYSAWRQEGSQLHLA
                     LVIEPESGAILVTVDGLSGAPEVLQASFNEGAIPVQAPRPLEFALAQNTPNPFNPSTV
                     IRYTLPENALVQLTVFSTTGQVIRTLVRAQVEAGSHQAVWDGRDDHGREVASGTYVCR
                     MAAGRYNSVMRMTLAR"
     gene            complement(7008..>7767)
                     /locus_tag="PLT86_10625"
     CDS             complement(7008..>7767)
                     /locus_tag="PLT86_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT37106.1"
                     /translation="PSLTELVFALGAGSRLAGVTDFCDYPPAARTLPRVAGFNVVNLE
                     ALVAARADLVLANRGNSPTDLAAIRELGVPVFAFQIDSLPELARAARTLGGILGVQAE
                     AESLATSWERRIASVQSVGDSVPENARPLVFFGGIAEPIYTVSRGSFIHDVIQRAGGR
                     NVFADLPTAWPRIDLETLVRRNPDIILVGYHAATERDIASLREAPGWRTIKAVREGKV
                     YVLGDAIMREGPRLVDALEEVSRVLHPRGKVLSQ"
BASE COUNT         1494 a         2299 c         2546 g         1428 t
ORIGIN      
        1 cgacctgttc cagtgtcacc ctttccatca ccggcatctt cacgtcgccg acctggcagg
       61 tgtacccgag cacggacacg ccttcgtacc ccagcagctt cttcgcaatt gccccggcgg
      121 ccacacggcc tatggtctcg cgggcagaag ccctgcctcc gccgcgataa tcacgcacgc
      181 cgtatttggc gaggtaggtg taatccgcgt gccccggtct gaaaaggtcc ttgatggcag
      241 cataatcgcg gctgcgggcg tcttcgttcc gcaccagaac ggcgataggg gttcccgtcg
      301 tcacgccttc gaacaccccg ctgagaatct cggcggcgtc ggtctcctgc ctcgcgctcg
      361 cgataagact ctgaccggga cggcgccgat ccagttcgcg ctggatgtct tcctcggcga
      421 gagggattcc gggcggacat ccgtcaacca ccacgcccac ggcggggccg tgggattcgc
      481 cgaacgtggt gatccggaat gaatggccaa agctgtttcc gggcatgatg cggtctcctt
      541 ttattgtcgt gtcaaagatg ggcacaacgc tctgtgcggg caaggcaggg gaccttcaaa
      601 aaaccggagt ttgaaatgac acaaacgcgc acatattttt ggcaagacgg tgtatttttt
      661 gaatcaaggc ggatagtcac ccggccggtg ggtgaaggtc caaccatgtg cgttgcgtac
      721 ggaagactga gtccgttttt tcggtgtcac atcctgtccg atacatgatg ttcatatcgc
      781 catcgccagc acaccgcaca gacagactgg ctgccgggca cgcagccaac gcacatgggg
      841 ggtccctgta tgctccgtgc agtccttcgc gctgtcctcg ttcttgccgt gtcgctcctc
      901 gggcctgtcg cacacgcaga aaccactgtt tccggtgaaa taaccactac cacgtggacc
      961 aaggcaaaca gcccgtaccg tgtcgccgga gacctgtaca tcccacccgg caatacgctc
     1021 acgatagagc agggtgttga tgtcatcttc gacgccgatg ccttgttcac cgtggagggg
     1081 atgatcacgg ccacagggac ggccgctgac agcatccggt tcttcccctc cggcgacggt
     1141 tcgtggcggg gattccgggt gtattccgaa ggtatggttg cagcgaaacc gctttctgag
     1201 cgggcgaaag cggcgcgggt gacgcaggaa ggcggcgcct ccattgagat ggctttcgcg
     1261 cggatcagcg gcgtgcagga cgcggacgac gaggcgaccg gcggggcatt cgaagtggtc
     1321 ggcgccgcat ccggcctcac gctcaccaac tgcgtgatac gggataacac cgcctacgaa
     1381 ggggcaggga ttgcgtttgt ggggggtgcg ggcgggatgc tttccctcgt cacgtttgcc
     1441 aacaacagcg ccagtaacca gggcggagcc atttacctca gcgacgcgtt cgtgcaggcg
     1501 atcagttgca cgttcgtgga taaccacgcc gtgaatttcg gcggagccat tgccgggata
     1561 gactacgaca caatcggacc tctctaccgg tcggctgccg gcagatccgg gcagaatggc
     1621 gacgggcgcg gaaaacggcc ttccggtgcg gaacggccgg cgaggccgga atctcccgtg
     1681 ccacgagttg cccgccgggc cgaggacggg gagccgtcct tcataatgct ggtgctggcg
     1741 cagattcgga acaacacctc cggggattgc ggcggagcgc tgtatctcca gggcaccgtc
     1801 ggcgcactca tgggttcgac agtggctcag aacagcacga cgtatgacgg tggtggcatc
     1861 tacgcgacca acggcttcgt gcagatgcaa gggtgctcgg ttgacagcaa cagcgcgggt
     1921 caggatgcag gcggcgtcgc gctctactcg cagactatgg ccggcatcac ggacacgcgt
     1981 tttcgcgcga atcaagccga aggagacggc ggcgcgctca acctttggga cggcgccaag
     2041 ctcactatcg aaacgagcac gttcgagaac aaccggtcca gtgacgacgg cggggcgatg
     2101 acggtgtaca ccggcagcac cgccacggtg cgcgagtgcc agttccgcgg caacgccgcc
     2161 gggtacgatg gaggcggaat catcgtctgg gatgactgcg aactgacggt ggaaaacacc
     2221 cagttcgaag gcaactccag cacaagcgac ggtggcgctc tggtggtcta caccggaagc
     2281 acggcgacca tttcggagag cacattccag gggaacacgt gtgatgacgg tgagggagga
     2341 ggatacggtg gcgcatttga ggcgtggcag aatgtcaccg tgacaatggg ttcctgcacg
     2401 ttcacgggca actttgcgcc ggaatcggga ggtgcggtcg gcatttacga ccaatcagcc
     2461 cttacgttcc gcggttgcga tttcggtggc aaccacacgg agacattcgg cggagcgctc
     2521 gacgtgtggt acagtgacct ccagcttgta gactgcgcgc tccgcaacaa cgcggtgacg
     2581 gggcccgggt acggcggcgg tgtgtacgcg ttcaattcgc ggattgcggc ctctaacgtg
     2641 ctggtcgcga agaacgaagc agactacggc ggcggaatgt atttcgacgc agatgcgtcc
     2701 ctcgcgctgg acagaggcac agttgcccac aatgtggggc gaacgggtgt gggaggcctc
     2761 ttcctttacg gccccgggca ttccattgcc agcagcatta tctggggcaa caccggtggt
     2821 aatgtctatg ttaccgatca ggcgaacgtg accgtcgcgt actcagacct tccttacgaa
     2881 ggtgtctggc cgggcacagg caacatcaac gccgacccga tgttcgtgga cgcggcggga
     2941 ggggatttca ccctcgcgga agggagcccc tgcatcaacg cgggcgatcc ggcgggagat
     3001 cctgatccgg acggttctct gccggacatg gggtggtcac cgacaggtgg cggcagcatc
     3061 ggcggcactg aggtatcggg tttgcttacg accggcacgt ggacacgcgc caacagcccg
     3121 taccgtgtca ccggcgacct cgaacttccc gaaggggagg tgctcaccat tgagcctggc
     3181 gtcgacgtga tcttcgagga gccggccttt ttctacgtgt acgggcgact gatcgctcac
     3241 ggcacggaga cggacagtat ccggttctat cctgcgtcgg cggcagcagg ctggcacgga
     3301 ttcgaaatcg aatcgtacgg cgggatgtac cggccactct ccactcgcgc aaaggctacg
     3361 cgtgcggcat acgaaggggg tgcttccgtt gagatgtcct acgtgcgcat cagcggcgtt
     3421 cgggacattg ccgactacaa cgacggtggc gcgttcatgg tgtacggcaa caccgcgtcc
     3481 gtttcggtca gcaacgcgat catccgggat aacgaggcgt acgacggtgg cgccgccaac
     3541 tttgtcgatg gcgcaatggg tcagttcacg aatgtcatgt tcgcagacaa ctactccgag
     3601 ggcgacggcg gcgcggtgac gttcaacgac gcgtttgtga gcttcgaggg atgcacattc
     3661 gcacgcaacg agagctatta cgccggcgga gcagtctacg tgtatgatta cgacggcggc
     3721 ggccttgaca aacccgcggc ccgtgcgaaa gcggacaaac acggcaaggc agatcggcgc
     3781 gccgtcgagc gtccctcgag aagggctgac cgtctctcgg ctcgtcccgc gcggccgacc
     3841 cggccccatc gggcagcccg cgaagatcgg tccacgaagg tttcccgtga ccagcggcgc
     3901 gcgctgcccg gaagggccgc aagggccgca aggccaactc ggcaagccac ggatggggaa
     3961 ctctcgtatc tctacgtgga agactgccag ttcgtggata actactccga cgaggatggc
     4021 ggcgcgctct acctccatgg cgttcagggt gagttctccg ggacttcgtt cgaacggaac
     4081 gaggcgtact gggaaggcgg ggcagtgtat tccgattact cggcgctgtc cttcaccctg
     4141 tcgtcctttg agggcaacta cggcccggac gagggtggag cggtggacgc ggggtactcc
     4201 acgctgtcat tcgacagttg cgccgtcaag aacaacgttt ctgacgggta cggtggcggt
     4261 ttttcgctgg atgggtcggt ctacctcacg gtcaatcgca gccttttcga gggcaactcg
     4321 accgacgaag gcggtgggat tgttgcctac gacgactgcc atatggtcgt tcagaacagt
     4381 gcgttcatcg gcaactccgc ctggagctac ggcggcgcga tggcagtgaa cagcagcagc
     4441 acgcttgagg tctcgaatac ctcgttcgtg gataacacct ctgaaggcac ttctggcggc
     4501 atcgaggtat gggattactc cagtgcctcg gcggatggct gcaccttcac cgggaacgtg
     4561 ggaattgacg gcggtggtgg gatgggggtg tacgacgacg cgaccgtggt tgtcagcaac
     4621 agcacgttcc gcaacaatgg cagtgccttc tccggcggcg gcgtcgatgt ctatcaggct
     4681 ggtgccacca tcgtcgagtg cgtgttcgaa aactgcactg cccaaaacga cgcagattgg
     4741 acctacgggg gtgcgttgta cgcgaacgac gcccaactgg acgtgggtcg aaccctgatc
     4801 gcgaagagca ccgcggacca tggcggcggc gtctgccttg agatggatgt aaccgcgacc
     4861 ttcacgaact gcaccatcgc cgacaacacc ggtgttgagg cgggcggcgg catctccctc
     4921 tacgacggtg tggtcaccct gaatctcctc aacaccatcg tcgcgtggaa cgagggcggg
     4981 aacgtgctca atccgcccga aggactcggg caggttgttg ccacctattc cgacattttt
     5041 gacgaagagg tctggccggg agagaggaac atcagcgccg atcccttgtt tgttgacagg
     5101 gaggcgggcg actatcggct ggcggcaggt tccccctgca ttgacgccgg cgatccagaa
     5161 agtgaacgtg accccgacgg aaccattgcg gatatggggg ccttcccgac cgagaagcag
     5221 ggcggcccga caccggtgag cgggaccatc ctgaccaccc tgtggaaatc gacaaaaagc
     5281 ccgtaccgcg tcgtcggtgc gatcaatgtt cccgcaggga acgtgctgac gatcgaggcc
     5341 ggagtggatg tgctgttcga cgcggacgtc gacttcatcg tgaacggcaa aatcgtcgtg
     5401 acgggcactc aaaccgacag cgtacgattc cttgccggag cgacagaatg gggtggcttc
     5461 agcattttga gccccacgga tacctcgctg tttcggtatg cgcgcttcag cggcgcgcgg
     5521 gcaaactcgg ggcaatatga cggtgccggc gcgatgtaca taaacgccag caaagcaatt
     5581 cttgaacact gcgtcgtgag cggcaactcc ggaacgtact gcggcggcgg aatggggctt
     5641 gacagcgacg ccatggtctg gatgagcgat tgcgcggttg tggacaactc ctgcggccat
     5701 gatggagggg ggatctactc tgcccgctca acgcttcaga tgcagcggtg cgtggttgcg
     5761 cggaacagct ccggcggcgc cggggacggc attcaggtag gatttgattc agtcgtggac
     5821 ctggtgaact gcacggtggt tgcaaacggc ggagcggcgt tctactggta tccagcaggc
     5881 acgcttcgcc tgaagaactg catcgtatgg ggcaattcca gcgcaggtct gatcacaagt
     5941 gcgacgtaca gcgacatcca gggtggcgtt ggaggagccg gaaacatcaa cgccgatccg
     6001 ctctttgtga atgccgccgc cggggattac cgtttgagcc cgggatcgcc ctgcatcgat
     6061 gccggggatc cggcaagtcc gcgggatcct gacctcacgc gggcggacat gggcgcgttt
     6121 ccgacaggaa taccatcgaa atacggcgac gtcaccggcg acggcacggt atcagcgggc
     6181 gatgcatcgc tcgtgcttcg gtacgtggtg gggattgttc cctcgttgcc ccgccccgtg
     6241 gctgctgacg tcactgccaa cggttcaacg agcagttacg acgcagccct cattcttcgc
     6301 aaagtactgg acgacacgta tctcttcccc gcggaacgag ggggtatgcc cggaacaaaa
     6361 atggccggaa ccctgccgcg ggtgctgagc tgggaacgcg ccgggtctgg ctgggcactg
     6421 cgggtaaacg atccgacggg aatccttgcg ggcgatttcc agtttgcact ccccgatgca
     6481 cgcgaggtga acgtggcggc gggggaatac tccgcgtggc ggcaggaggg ttcacagctt
     6541 caccttgcgc tggtgatcga gcccgaatcg ggcgccatac ttgtgaccgt tgacggcctt
     6601 tcgggtgcgc ccgaggtgct tcaggcgtcg ttcaacgagg gcgcaattcc ggttcaggcg
     6661 ccgcgcccgc ttgagtttgc gctcgcgcag aacactccca acccgttcaa cccgagcacc
     6721 gtcatccgtt acacgctgcc cgaaaacgct ctcgtgcagt tgacggtgtt cagtacgacg
     6781 gggcaggtta tcagaacgct ggtgcgcgca caggtggaag ccgggtcaca ccaggctgtc
     6841 tgggatgggc gcgatgatca tggcagggag gttgcaagcg gcacgtacgt ctgccgcatg
     6901 gcagcaggca ggtacaactc ggtgatgagg atgacactcg cgagataggt gggtacgcgt
     6961 atcaaccggc ggagcggcgc tgatggctgc tccgccggtt gttttggtta ttgcgacaag
     7021 actttcccgc gggggtgcaa cacgcgcgag acctcctcca gcgcgtccac gagcctcggt
     7081 ccctcccgca tgatggcgtc cccaaggaca taaaccttcc cttcgcgcac cgccttgatc
     7141 gttctccagc ccggcgcttc gcgcagggat gcgatatccc gttctgtcgc cgcatggtat
     7201 cccacaagga tgatatcggg gttccggcgg accagggtct caagatcgat gcgcggccat
     7261 gccgttggca ggtcggcgaa gacgttccgg cctcccgcgc gttgaatcac gtcgtgaatg
     7321 aacgacccgc gggaaaccgt gtagatcggt tcggcgatgc ctccgaagaa gacgagggga
     7381 cgcgcgtttt cgggtacgga atcccccacc gattgcaccg aggcaatcct ccgttcccac
     7441 gaggtggcga ggctctccgc ttcggcctga accccgagga ttcctcccag agtccgcgct
     7501 gcccgggcca attcggggag cgagtctatc tggaatgcga acacgggaac tccaagttca
     7561 cggatggccg cgagatcggt cggggagttg cctcggttgg caagcacgag gtccgcgcgt
     7621 gccgcgacaa gcgcttccag attcacgacg ttgaaaccgg ccacacgcgg cagagtccgg
     7681 gcggcgggcg ggtaatcgca gaagtcggtc acccccgcca atcggcttcc ggcaccgagc
     7741 gcaaacacca attccgtgag actcggc
//
LOCUS       DAOSOO010000158         7761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9386_length_7761_cov_5.267584, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000158 DAOSOO010000000
VERSION     DAOSOO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_7761_cov_5.267584"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLT86_10630"
     CDS             <1..1012
                     /locus_tag="PLT86_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37107.1"
                     /translation="PDLNEIFDERTGARIVRLTPPAGLHYHQYCYGQWIAPDCRTLLV
                     FRYRELRRDSPIDLYRVNTDGKGYEIVAENAGWSTISVDGQFVFAGTAGGILRIPLSG
                     GESDTVFRSDSYRGMSANAQTRDGRFLFCHAFGERGHELVRVQLDTGEAQVLFKTDYL
                     MHLHLHDEGEGRLLATVRPPDLEWGIYTFDLDGGDFRKLPFTRSTNHFASLGCTGKVI
                     TTVHGGGNAIEIGTPGGASAEVLVEGEGFWHPTADSTGEWVACDTNWPDTGLMLVHVP
                     TRRYRVLCFTGTSGGHPQWAHAHPRMSPDASYVVFDSDKSGITQTYAVFVPPEFKEEL
                     RG"
     gene            1061..2098
                     /locus_tag="PLT86_10635"
     CDS             1061..2098
                     /locus_tag="PLT86_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37108.1"
                     /translation="MGCERVARTDPETGVRILQLTSFPITHWHLGLGDFITPDGERLL
                     IAANRFAGRNSPRDLYSIRADGDDFNFLLHDFSGGVICPRVQWVYTGCAGGVVRIPIE
                     GGTAEVLWSPDCYTHIMVSARSSDGLWLFGQGRRKTDGSFDIVRISTETGEASVIVRA
                     SYVMPVLRSSANGERLVASIAPVGDDGKPLMPWGVWEFSSEGTDFRLVPFSRSTNHYC
                     PMGSTGLVVTTAAHPGNALDVAAPGGCERVLAHGAGFWHVSSDETGTWLASDTNWPDV
                     GLQIVNSHTGKYATLCMTGASGGHPQWTHAHPVISPDARWVLFTSDRTGFQHVYLADI
                     PPEFKKALSAA"
     gene            2408..3778
                     /locus_tag="PLT86_10640"
     CDS             2408..3778
                     /locus_tag="PLT86_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT37109.1"
                     /translation="MPSNLRLLLADGDRSSLTIMQEITAQWGHQVLACRSVAEAVSAM
                     DAHKVDVVVTDLVLPDGSGLDLVRHAHEIDVPVASIVISAHGTIAGAVDAVRLGAYDF
                     LTRPVDLAKLKVIIPAAFDKERLRFENRQLRSKISEETRPEGLVGQSLAIRQVIEKIR
                     MAANARATVLITGESGTGKEVIATAIHKLSPRSAEPLVKVATGALAPQLLESELFGHE
                     RGAFTGAVAQRRGRFELAHTGTLFLDEIDGLEIGLQGKLLRVLQERQFERVGGEQTIT
                     VDVRLIAATNANLEKMVEQGLFRKDLYYRLNVVRIEAPALRDRREDIPLLAYHFVALY
                     AQENGKTIRGIDPAAMALLQSYEWQGNVRELENAIESAVVMCPGDWITADLLPQNVRS
                     GPPEEMQFRVGMTLEEMERRAIEATLRATHGNKTAAAKMLGIGVRTIHDKIKKLRPAG
                     ASEPTE"
     gene            3854..4243
                     /locus_tag="PLT86_10645"
     CDS             3854..4243
                     /locus_tag="PLT86_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37110.1"
                     /translation="MGPASASEASGHTTQPEWFPAVVELLRVRGYLTLSVKIGYELVL
                     IVSGSAGEAAVIDLSQEPTSLWTDAWAGMRQVLPRVPVIAILPAGNTDETIMPEHGAV
                     ARVVSPVDAESLVGIVEHVLQNPSYSR"
     gene            complement(4294..5490)
                     /locus_tag="PLT86_10650"
     CDS             complement(4294..5490)
                     /locus_tag="PLT86_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT37111.1"
                     /translation="MPKVLSSRLQRLPPYLFARINGMRLQLRQQGMDIIDLAMGNPDR
                     PTPKHIVDKLIEVSQDGKAHRYSASRGIPALLKAIARYYDRKYGVSLDWEKEVVATIG
                     SKEGIGHLVLALIDPGDLALVPNPAYPIHIYSVALAGGNVVTVPLHEEQDFVPSMAEI
                     TREIWPKPRIITLNFPNNPTTATVSLDFFQEVVDYAKKNDIIVIHDMAYSEITFDGYE
                     SPSFLQAKGAKDVGVEFFTLSKSYNMAGWRIGACVGNADVVGALAKIKSYYDYGIFTP
                     VQVAGIAALDGPQDCVREAAAVYQARRDVLCEGLNRIGWPVKKPKASMFVWAPIPEPY
                     RHMGSMDFSLMLMEKAEVAVAPGIGFGDLGDDYVRIALVENEHRLRQAIRNIRRSLTE
                     LKPADT"
     gene            5637..7388
                     /locus_tag="PLT86_10655"
     CDS             5637..7388
                     /locus_tag="PLT86_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37112.1"
                     /translation="MKRLNNMVVELARGVPKASEELFECEFESPRSGWHYVGCSWDGE
                     SGEVRFVVDGRAAGTRPAFGSLVEAMPQLPAGPHTLRVEKTADGRLGEVCVRAVPELA
                     YCKHQYNPHIPEYGPYDWDFIARTVLPHVNTVVGNGDPSHEEHLKWWKARGGKWIVET
                     SLHGMNDETVTADDVWRNWTSSPGMVHPLMDGIMVDEFFSGRRPQYKAWTEAMSRIAS
                     DKRFEGKKVYPYLGSHYPDMDDWKRNASDGVDSSYRFLATTMEAGYPIAWERYLQERH
                     SEDCAEEHIQDRLGSTIAHWRHRFPDCARSLLVCLGYMTITETLNIHPGADYKVFMDM
                     QFRFLANHPDFEGLFGILEYTSGYADEETLRWAARLYRHYGIEGETDLLSDRLGYSYV
                     PGHLRNPDFAFGWEGWHVPVDIEENVHVRHCEGFGHLQGRWPRTSVGDSFLTMKRDAK
                     IPNVVSQRIKNLQPGRMYSAKMISGSYDQLLAGKSKPEKLGIALSIAGVETIPKKTFQ
                     SVVGNNYSHELGAFEGQNRYWFNYHQVVFRATSPSAELFISDWEGSSSDGRPAGPLGE
                     TLLCNFVEVQPYFEEGV"
     gene            complement(7411..>7761)
                     /locus_tag="PLT86_10660"
     CDS             complement(7411..>7761)
                     /locus_tag="PLT86_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT37113.1"
                     /translation="QEHVEAAGFSVVRDFCGHGIGRMFHEDPQVLNYGQRNTGRKLRP
                     GMVFTVEPMINAGTWEVRILRDGWTVITQDGSLSAQFEHTIAVTASAPRVLTLFTEHE
                     HAMWASYQKPDRAG"
BASE COUNT         1628 a         2162 c         2357 g         1614 t
ORIGIN      
        1 gcctgacctg aacgaaatct tcgatgagcg aacgggcgcg cgcatcgtgc gtctcacccc
       61 gccggcaggc ctccattatc accagtactg ttatggccag tggatcgctc cggactgccg
      121 gacgcttctc gtattccgct accgggagct tcggcgcgat tccccgatag acctgtaccg
      181 cgtcaatacg gatggcaagg gatacgagat tgttgcggag aacgccgggt ggtccaccat
      241 ctcggtggac gggcagttcg tttttgccgg taccgcaggc ggcattctgc gcattcctct
      301 ttccggcggc gaatctgaca cagtgtttcg ctcagattcc taccgtggaa tgtcggcgaa
      361 cgcacagacg cgggatgggc gatttttgtt ctgtcacgca ttcggcgaac ggggacacga
      421 actggttcgt gtgcaactcg acacggggga ggcgcaagtt cttttcaaga cggattacct
      481 catgcacctt cacctgcacg acgaaggtga gggtcggctt ctcgccaccg tccgtcctcc
      541 cgatttggaa tggggaatat acacattcga tctagatggg ggagatttcc ggaaacttcc
      601 gttcacgcgg agtacaaacc atttcgcatc gctcggttgc accgggaaag tgattactac
      661 ggttcacggc ggcggcaacg ccatcgagat cggtacccct ggcggcgcct ctgccgaagt
      721 gttggtggag ggcgaagggt tctggcaccc gacggctgac tcaacgggcg aatgggttgc
      781 gtgcgacaca aactggccgg atacgggctt gatgctggtg catgtcccga ctcggcgcta
      841 ccgcgttctc tgcttcaccg ggacgagtgg cggccacccg caatgggcac atgcgcaccc
      901 gcgaatgtca cccgacgcga gttacgtcgt gttcgacagc gacaaatcgg ggatcactca
      961 gacgtacgca gtcttcgttc ctcccgaatt caaagaggaa ctccgaggct gaccgaaccc
     1021 tatcgcagcg tttcttcctc attgcagcgg ggtattgtcc atgggttgtg agcgcgtggc
     1081 ccgaaccgac cccgaaaccg gcgtcaggat tctccagctg acgagctttc ccatcactca
     1141 ctggcatctc ggccttggag acttcatcac gccggatggt gagaggctcc tgattgcggc
     1201 aaacaggttc gcgggaagga actcgccgcg agacctgtac agtattcgag ccgatggaga
     1261 tgattttaac ttcctgctgc acgatttttc cggtggtgtc atctgcccgc gggtgcaatg
     1321 ggtttacacc ggctgtgcgg gcggcgttgt gcggattccg atcgaaggcg gaacggcgga
     1381 agttctctgg agccccgatt gctacacgca tatcatggtt tccgcgcgga gcagcgacgg
     1441 gctgtggctg ttcgggcagg gccggcgcaa aaccgatggc agcttcgaca ttgtcaggat
     1501 ttcgacggaa accggggaag cctctgttat agtgcgggcg agttacgtca tgccggtttt
     1561 gcgcagcagc gcaaacgggg aacgactggt tgcgtcgatc gcacccgtcg gagacgacgg
     1621 caagcctctg atgccgtggg gcgtgtggga attctcgtcg gaaggaaccg atttccgcct
     1681 cgttccattc tcgcggagca cgaaccacta ctgcccgatg gggagtaccg gcctggtcgt
     1741 aacgaccgcc gcgcatccgg gcaatgccct tgatgttgca gcccccggcg gatgtgaacg
     1801 tgttctcgcg cacggtgcgg ggttctggca tgtcagttct gacgaaaccg gcacttggct
     1861 cgccagtgat acaaactggc ctgatgtcgg gttgcagata gtgaactcgc acacggggaa
     1921 gtatgccaca ctctgcatga caggcgccag tggcggacat ccccaatgga cacacgcgca
     1981 cccggtgatc tcaccggatg cgcgctgggt gctgttcacg agtgaccgaa cgggcttcca
     2041 gcacgtgtat ctcgccgaca ttcctcccga gttcaaaaaa gcgctgagcg ccgcatgata
     2101 cgagccttcc acgggcgcgc gaaaggttcg tgaggccgca tttgaaacag ggtaagcggc
     2161 taacacgcgg atgcagtccg cgcaacaagc gcctgcgaca gtttttcccg gcccggattg
     2221 acgagagtct gcaaaactcg ctacattgta gtgtccattg ctgcgcattt gtgcaaaatt
     2281 cgccggctac acgaacgaat gataggtcgt aatcgccggt tggcgcttct gtattgcacg
     2341 gaatcatcct gatctcatcg cagatgggac aaaaacttcg cgttcctgca aacccggggt
     2401 cgtgttcatg ccttcaaacc tgcgtctgct cctggcggac ggagatcgca gttccctgac
     2461 gatcatgcag gagattacgg cccagtgggg gcaccaggtt cttgcctgcc ggagcgtggc
     2521 ggaagccgtt tccgccatgg acgcccacaa ggttgatgtg gtggttaccg acctggtcct
     2581 cccggatggc agtggactgg acctcgtcag acacgcacac gaaatcgacg ttccggtcgc
     2641 ctctattgtg atttccgcac acggaacgat tgcaggagca gtggatgccg tacgtctggg
     2701 ggcatacgat ttcctgacgc gcccggtgga tctcgccaag ctcaaagtga tcattcccgc
     2761 ggcgttcgac aaagaacgcc tccggttcga aaatcggcag ctccgttcaa aaatcagcga
     2821 ggaaacgcgg ccggaggggc tggtaggcca gagtctggcg atcagacagg ttatcgagaa
     2881 aatccggatg gcagcaaatg cccgtgctac ggttctgatt accggtgaaa gcggtacggg
     2941 aaaagaggtt atcgccactg cgattcacaa attgagtccg cggagcgcag agccgctggt
     3001 gaaggttgcc acaggcgctc tggctccgca gctgctggaa agcgaattgt ttggccatga
     3061 gcgcggcgct ttcacgggtg cggtggcgca gcgtcgcggg cggttcgaac ttgcgcatac
     3121 gggaactctg ttccttgacg aaatagatgg tctggaaatc ggcttacagg gcaaattgct
     3181 cagggtactc caggaacgac agtttgagcg ggttggcggc gagcagacga ttacggtgga
     3241 cgtccgcctt atcgccgcga cgaacgccaa tctggaaaaa atggtcgaac aggggctgtt
     3301 ccgaaaggat ctctattacc ggctgaatgt cgtcagaata gaagcaccgg cgttgcggga
     3361 ccggcgagag gatatccctc tgctcgccta ccactttgtc gcgctctacg cgcaggagaa
     3421 tggaaaaaca atccgcggaa ttgaccctgc cgccatggca ttactccaga gctacgaatg
     3481 gcaggggaat gtccgggaac tcgaaaatgc gatcgagagc gcggtggtga tgtgtccggg
     3541 agattggatt actgcggatc tcctgccgca gaatgtgcgc agtggaccgc cagaggagat
     3601 gcaattccgt gtaggtatga ccctcgagga aatggaacgt cgtgcgattg aagcgacctt
     3661 gagggcgact cacggtaaca agactgctgc agcgaagatg ctgggtatcg gggtcaggac
     3721 gatccatgac aaaatcaaga agcttcgccc cgccggagcc tcagaaccaa cagaatgaac
     3781 ctgcgacggt acggagattg catcacgtga cttcgtccgg acactcggaa tcgcgagcat
     3841 gaggagcaac tgaatgggac ccgcgagcgc atcggaagct tccggtcaca ctacgcaacc
     3901 ggaatggttc cccgctgtcg tagagcttct gcgcgtccga ggctacctta cgctttccgt
     3961 gaagatcggc tacgaactgg tgctgatagt cagcggatcg gctggcgaag cagcggtcat
     4021 tgacctctca caggaaccga cctcactgtg gacagatgcg tgggcaggca tgcggcaggt
     4081 gctgcccagg gttcccgtga ttgcgattct gccggcgggg aacacagacg aaaccatcat
     4141 gcctgagcac ggtgccgttg cgcgtgttgt gtcacctgta gacgcagaga gccttgtggg
     4201 tatcgtggag cacgtcctgc agaacccctc atattcacgt tgaccacgcc ttcgctcctg
     4261 ccggtttgtc cacaccacca agctgaccac tgtttacgta tccgcaggct tgagctccgt
     4321 gagcgaccgc cgaatgttgc ggatcgcctg cctcaggcga tgttcgtttt ctacgagtgc
     4381 gatgcggacg tagtcgtcgc caaggtcgcc gaacccgatg ccgggagcca ccgcgacctc
     4441 cgccttttcc atgagcatca gactgaaatc catactcccc atgtgccggt acggttccgg
     4501 gatgggtgcc cagacgaaca tcgatgcctt gggtttcttc accggccatc caatgcggtt
     4561 cagcccctca cacagtacat cccgccgtgc ctggtacacg gcagccgctt cacggacgca
     4621 gtcctgtggc ccgtccagtg ccgcaatccc agccacctga accggggtga aaatgccgta
     4681 gtcgtagtag ctttttattt ttgcgagcgc tccgaccacg tcagcattgc ccacgcacgc
     4741 tccgattctc caacctgcca tgttgtagga tttggaaagg gtgaaaaact ccaccccgac
     4801 gtccttcgcc cccttcgcct gaaggaaaga ggggctctcg tatccatcga atgtgatttc
     4861 ggaatatgcc atatcgtgga tgacgatgat atcgttcttc ttcgcgtaat cgacgacttc
     4921 ctggaaaaaa tccaggctta cagtagcagt ggtaggattg ttcgggaagt tcagggtgat
     4981 gatgcgcggt ttcggccaga tttcgcgcgt gatttcggcc atgctgggga caaaatcctg
     5041 ttcctcgtga aggggaacgg ttaccacatt tccccccgcg agcgcgacgc tgtaaatgtg
     5101 tatcgggtac gcggggttcg gaacaagcgc caggtccccc gggtctatga gggccagaac
     5161 aaggtgtcct attccttctt tcgagccgat ggtggcgacg acctcctttt cccagtccaa
     5221 gcttacaccg tactttcgat cgtagtaccg ggcgattgct ttgagaagcg caggaatccc
     5281 gcgtgaggcc gaatagcggt gggctttacc atcctgagag acttcgatga gcttgtccac
     5341 aatgtgtttt ggtgtgggcc ggtcaggatt tcccatcgca aggtctatga tatccatgcc
     5401 ctgctgacgg agctgaagtc gcatcccgtt gatgcgtgcg aaaaggtacg gtggcaggcg
     5461 ctgcaggcga ctggaaagga ctttgggcat ctgcttcctc cagatggtaa acgcgtaagg
     5521 aacgattttc aaagatagca aaccgacgac gaagaataca gcgcatcacc cgaaaaagcg
     5581 ccttcggagc gatggtctgc acacctcctt ttcacctgtt cgtgaaggac gactgaatga
     5641 aacgcctcaa caacatggtt gtggaactcg ctcgcggcgt tccgaaagcc agtgaggagc
     5701 tttttgaatg tgaattcgag tccccccggt ccggctggca ttacgtcggc tgctcatggg
     5761 acggggaaag tggagaggtg aggttcgtcg ttgatgggcg tgcggcgggt acgcgccccg
     5821 cgttcgggtc gctcgtggag gcgatgcctc agctgccggc agggccgcac acattgagag
     5881 tggaaaagac agcagatggc cgattggggg aagtgtgcgt cagggcggtg cctgagctgg
     5941 cgtactgcaa acaccagtac aacccccaca tacctgagta cggcccgtac gactgggatt
     6001 tcatcgcgcg aaccgtactt ccccatgtga acacggtagt gggaaacggg gacccaagcc
     6061 atgaagagca tctgaagtgg tggaaagcca ggggaggaaa atggatcgta gaaaccagcc
     6121 tccacgggat gaacgacgaa accgttacag cagatgacgt atggcggaac tggacatcca
     6181 gccccggaat ggttcacccg ttgatggacg ggatcatggt ggacgagttc ttctccgggc
     6241 ggcggcccca atacaaggcg tggacggaag cgatgtcccg aatagcttcc gacaaacggt
     6301 tcgagggtaa gaaggtctac ccgtacctcg gatcgcatta tccggacatg gatgactgga
     6361 agcgcaatgc gtcggacgga gtggactcaa gttaccggtt cctcgcgacg acgatggagg
     6421 ctggttatcc cattgcctgg gaacgttacc ttcaggagcg ccattcggag gattgcgcgg
     6481 aggaacacat acaggatcgt ctgggaagca cgatcgccca ctggcgacac cggttccccg
     6541 attgtgcccg ttcgctgctc gtgtgcctcg gatacatgac gataacggag acgctgaaca
     6601 tccatccagg ggctgactac aaggtcttta tggatatgca gttccgattt ctggccaatc
     6661 atccggattt tgaagggctt ttcggcatcc tcgaatacac cagcggatac gcggacgaag
     6721 aaacccttcg ctgggcagcc cggctctacc ggcactacgg gatcgaaggg gaaaccgacc
     6781 tgttgtcaga ccgtctgggg tactcctatg tgccgggtca tctccgcaat cccgattttg
     6841 cgttcggatg ggaaggctgg cacgtgccag tggatatcga ggaaaacgtc cacgtgcggc
     6901 actgcgaagg ctttggccac ctgcaggggc gctggcctcg aacctccgtt ggggacagct
     6961 tcttgaccat gaaacgcgac gcaaaaatcc cgaatgtcgt aagtcagcgt atcaagaacc
     7021 tgcaacccgg ccgaatgtac agcgcgaaga tgatttcggg gagctatgat cagcttcttg
     7081 cggggaaatc aaagccggag aaactgggta tcgctctttc gatcgcagga gttgaaacga
     7141 ttccgaaaaa aacgttccag agcgtagtcg ggaacaatta ctcacacgaa ctcggcgcat
     7201 tcgaagggca gaaccgctac tggttcaatt accatcaggt tgtgtttcgc gcaacttcac
     7261 cctcggccga gcttttcatt tccgattggg agggctcgtc ctccgatggc aggccagccg
     7321 ggccattggg cgagactctc ctgtgcaatt tcgtcgaagt gcagccgtat ttcgaggagg
     7381 gggtatgagg gaggtgttct gcgtgttgcg ctaaccggcg cggtccggct tctggtacga
     7441 ggcccacatt gcgtgttcgt gctccgtgaa gagagtcagg actctgggtg ccgatgcggt
     7501 gactgcgatt gtgtgttcaa actgcgccga gagactgccg tcctgcgtga tcaccgtcca
     7561 cccgtccctc agaatgcgca cctcccacgt tcccgcattt atcatgggtt ccaccgtgaa
     7621 caccatgccg ggccgaagct tgcgtccggt gttacgttgc ccgtagttaa gcacctgcgg
     7681 gtcctcgtgg aacatcctgc ctattccgtg gccgcagaag tctcgcacga cgctgaaccc
     7741 cgcggcttcc acatgctcct g
//
LOCUS       DAOSOO010000159         7630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9606_length_7630_cov_5.228779, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000159 DAOSOO010000000
VERSION     DAOSOO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9606_length_7630_cov_5.228779"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLT86_10665"
     CDS             <1..416
                     /locus_tag="PLT86_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37114.1"
                     /translation="VMDYFYCPKLAYFYLRRVQQPVCVMVDEPEDWRCRVVLGNDSLE
                     RVQGTFRIWDAATGETIWEGGGTPERNANTVLTAIPASRGQQRLLLIEWETEGIKGGN
                     HYLLGSPPFNGTWYRELLPAIAALPCGFDSLAVGK"
     gene            605..904
                     /locus_tag="PLT86_10670"
     CDS             605..904
                     /locus_tag="PLT86_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT37115.1"
                     /translation="MILGRVAGTVVATQKDEKLRSAKLLVVETLDPLTGEGKKAYTVA
                     IDAVGAGLGEVVLCASGSSARLTPSSDGRPVDTVIMAIVDQYDIHGKIVYRKSGQ"
     gene            956..2419
                     /locus_tag="PLT86_10675"
     CDS             956..2419
                     /locus_tag="PLT86_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOT37116.1"
                     /translation="MQIDEARVRAIVEQVVKQLGTPAGATGAKTPVNAPDSGAVNPPA
                     TTLGRGVFADIDEAVAATEKAQREWIAMPVAKRKEVVEAVRRVGIEHGEMFSRETVAE
                     TGMGRVADKIAKHRLASLQTPGFEDLVTTARSGDHGLTIEEMAPYGVIAAVTPSTHPV
                     PTMINNTICNLVAGNGVVFNGHPAGKRVFAHALQVINEYVVAAGGPANIITTVAEPTI
                     EGANKLFHHPAIKLILVTGGPGVVKAAMGAGKKVIAAGPGNPPVVVDETADIEKAARS
                     IIAGATFDCNLLCIAEKEIFVVDTVADELKRRMVANGAFELTTAQIQQLTSFVFKGDA
                     GCAHPVLNRDAVGRDADTLAKAIGLSVPSTTQLLIGETTKEHPFVQHEQMTSFVPIVR
                     CRDVHQAIEWAYEAEHGFGHTAIMHSKNVENMDKMAKVMNTTIFVKNGPSTAALGSGG
                     EGTSSFSIALATGEGITTPRTFTRIRRCALVDYFRII"
     gene            2441..2743
                     /locus_tag="PLT86_10680"
     CDS             2441..2743
                     /locus_tag="PLT86_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT37117.1"
                     /translation="MQLGRIIGTIVATQKIERLCGVRLVVLEPLNAQREPTGPPIAAI
                     DVVAAGVGDTVFWVGSLEAAQALEPPFPAVDASVVGLVDRVDLLPGKPASEETGAE"
     gene            2740..3039
                     /locus_tag="PLT86_10685"
     CDS             2740..3039
                     /locus_tag="PLT86_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT37118.1"
                     /translation="MIVGRVVGSVVSTVKHPAYASTKLLLVRPVSMDGVEEGPEMVCV
                     DTVGAGAGELVLVCKEGAAARQVLRKKHAPVRSLVIGIVDHVEAADGRRMIDGRD"
     gene            3063..3875
                     /locus_tag="PLT86_10690"
     CDS             3063..3875
                     /locus_tag="PLT86_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT37119.1"
                     /translation="MAIPLFQLKQQIVDACHRVYAKGWVASNDGNLSVRVDGERVLCT
                     PTGISKGLVTVDDLCLIDMEGNKLEGAAHRKPSTEMKVHLMAYKERPDVVSVVHAHPP
                     YATGYALAGLDLGECKLPEVVIALGTIPLTKYGLPGTDDLPNKMREFIHQSDAFLMEL
                     HGVLTVGQSVLNAYFKMETVEHFAKIDFIARQLGGARLFSKEQAEELLAARARYGVTT
                     PNIGCRAADGYAYPPDARDNLGPGANPGSGIPEDKLVEEVTKRVVQALKAKN"
     gene            3974..5173
                     /locus_tag="PLT86_10695"
     CDS             3974..5173
                     /locus_tag="PLT86_10695"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOT37120.1"
                     /translation="MHVLVVNVGSTSFKYRLLAMPEERVLAEGRLERIGSASSPVEFS
                     SGEHSEKSTIYLPDYAAAIGKMMSDLTSAKSPVESLKQIDAVGFKTVVIDGVPGCVLL
                     DDDAVSRMEALNDIAPAHNPPYVNAVRQMRALFPDLPLVGLFEPAFHVTMPDYAYTYG
                     VPHSWAEKYGVRKFGYHGASHRYVAETAPKLAGIDPSRCRLISAHLGGSSSLCAIRNG
                     QSIDTSMGMSPQSGVDHSTRNGDLDVFAVLHVMDKEGLSTSDVRRILCKEAGLAGISG
                     TSGDVRDLEAAAASGNDRARLALDVFCYNAKKTIGAMAAALGGLDVIGLAGGIGERGV
                     NVRKRILNGLDFLGVALDEERNSAHTGQEGVISTDASPVKVVVVRTNEELIVARSVFA
                     LLSGNAS"
     gene            5181..>7630
                     /locus_tag="PLT86_10700"
     CDS             5181..>7630
                     /locus_tag="PLT86_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37121.1"
                     /translation="MELVWAPERIGEGRRFRVIIRSDAPVRCFYSKHLELVDRTPEAH
                     NSLHFLYFRSVIPTTAAEIRCESGSESIVVRLPVIAEADWEHDARLHEIPLPRIWPME
                     RTYFPLKNRHTLVDGNEIGVRNSETQVSVNEGHAVDSAGSARRKQGDRTSCEALEWPD
                     EEIWNLEPQCDIPRWHFLNLDKGCPVHGLAIYSHNAYYPWIVDERNRPYHVKCPVGGE
                     WYPTNNLAEGDHTSGAFPDDGWGWHSPDGAVFGFTAYALLRRIRLVYGTVQSLAQHFK
                     GTGDQKAARKIVYLLISIAREHRYLAFFPEHRFRKYEEVVEEPHYREHKDRISWGPRE
                     TKTVANLAGGSGMDEYCINMLYHYEHLSRAYDMVFDRIDGDSELIAFVRARMPSLSDG
                     EKIRRYIETFLFRVGVQAALDDALMSNLPRPQMAMLDLIRLLDQPECKGLVDWLVSGG
                     GQVGRMPTNFYYKDGASYESLGGYNGIHVSALIPIVSGLRALRNEHPALYSSPAYDPI
                     AGHERFWHVLRFPFECVVAQTGYPLIGDSGDIPNTKVCRTVVCMDVGDPLAVYDEAVR
                     LFPDDTTFSTMRELLRRQASGEAIEPDARLMQPSRLLDGYGLGILESGDLENRRGVWL
                     YYGDHPGHSHEQPMDMGLFAHKRNILRHMGYPYSWQHMDTWDGNWITHFGVKVMASEP
                     PAWKNSIHFLFGSGPFQVVEASGYGLGNRETGEWEPLPGCVQRRTLCLLDLPDGRYYA
                     VDLFHVEGGDDHWWTFHGLPGEFTMSAELPAQEAGTVAGKDVAYGQTPPDGVPKSLAY
                     LYDVFRGQIEEPWSAHWLL"
BASE COUNT         1613 a         2079 c         2451 g         1487 t
ORIGIN      
        1 cggtgatgga ctatttctac tgcccgaaac tggcgtattt ctacctgcgc cgagtccagc
       61 aaccggtgtg cgtcatggta gacgagccgg aggattggcg ttgtcgtgtc gtactcggaa
      121 atgacagcct tgagcgtgtc cagggcacgt tccgcatctg ggacgcggca acgggcgaga
      181 cgatctggga gggcggcggt acgcccgagc ggaacgccaa cacggtgctg actgcaatcc
      241 ccgcaagcag ggggcagcaa aggctgctcc tcattgagtg ggagactgag ggcattaaag
      301 gcggtaatca ctaccttctg ggaagtcctc cattcaacgg aacctggtac cgggagcttc
      361 ttcccgcgat cgccgccctg ccctgcgggt ttgattcgct ggctgttggc aaatgaaacc
      421 tgcttacgtg caggggctag ccgcgcgcga gcaccatctt gccccgcctt cccggacacc
      481 gttattttgg ggggaggcaa accagttttt gtaccccgcg cggcgacagg taaaagtgcg
      541 tggtggctct attgccagat gaatcctggc gggtgtggcg tgagggattc gggggaatga
      601 ttcgatgatt ctcggacgtg tcgcgggcac cgttgttgcc acgcagaaag atgaaaagct
      661 ccggagtgcg aaactcctgg tggtggagac cctcgacccg ctgacggggg aaggaaagaa
      721 ggcgtacacc gtagcgattg acgcggttgg ggcgggcctc ggggaagtag tgctgtgcgc
      781 gagcgggagc tcggcccggc ttacaccctc ctctgacgga cgccctgttg acaccgtgat
      841 aatggcaatc gtagaccagt acgacataca cgggaagatc gtgtaccgga aatcggggca
      901 gtagccatat cgggatactt gttcggctta cgctgatcac agatggaggc gtacggtgca
      961 gatagacgaa gcacgcgttc gcgcgattgt ggagcaggta gtgaaacaac tcggcacgcc
     1021 ggcgggcgcg accggggcga aaactcccgt caacgcccct gattcagggg cagtgaatcc
     1081 tccggcgaca actctcggac gcggcgtttt tgccgatatt gacgaggcgg tcgcggcaac
     1141 ggaaaaggcg cagcgtgaat ggatcgcgat gccggttgcc aaacggaagg aagtggtgga
     1201 ggctgtccga cgggtcggga tcgaacacgg tgagatgttt tcgcgcgaaa cggtcgcgga
     1261 aacaggaatg ggacgcgtgg cggacaagat cgccaaacac cgcctcgcga gcctccagac
     1321 accggggttc gaggacctcg ttacgacagc ccggtcaggt gatcacgggc tcactatcga
     1381 ggaaatggca ccgtacgggg ttatcgcggc ggtgactcct tccacgcacc ctgttccgac
     1441 gatgatcaac aacacgattt gcaatctcgt ggcggggaat ggtgtggtct tcaatggtca
     1501 cccggcgggg aagcgggtat tcgcgcacgc actccaggtg attaacgagt acgttgtggc
     1561 ggccggtggg cctgccaata tcatcacgac tgtcgccgaa ccgaccatcg agggcgcgaa
     1621 caagctgttt caccaccctg cgatcaagct catcctggtt actggcgggc ccggagttgt
     1681 gaaagcggcc atgggcgcgg gaaagaaagt cattgcggcc ggcccgggaa atccgccggt
     1741 agtcgtcgac gaaacggcgg acatcgagaa agcggccagg agcattattg ccggggctac
     1801 gttcgactgc aatctccttt gcatcgcaga gaaggaaatt ttcgtggtgg ataccgtcgc
     1861 cgacgagctc aaacggcgga tggtagcgaa tggcgcgttt gaactgacca cggctcaaat
     1921 tcaacaactg acgtcgttcg tgttcaaggg agatgcgggc tgtgctcacc ccgtgctcaa
     1981 tcgggatgca gttggacggg acgctgatac gctcgcgaag gccattggtt tgagcgtgcc
     2041 atcgacaacg cagttgttga tcggggagac caccaaggag cacccgttcg tgcagcacga
     2101 gcagatgact tcctttgttc ccatcgtacg ctgccgcgac gtgcatcagg caatcgaatg
     2161 ggcctatgaa gccgaacacg gttttggcca caccgcgatc atgcactcga aaaacgtcga
     2221 gaacatggac aagatggcca aagtcatgaa cacaacgatc ttcgtgaaaa atggacccag
     2281 cacggcggcg ctgggctccg gtggagaggg cacctcatca ttctccatcg cgcttgcgac
     2341 gggtgaaggc attacgacgc cgcgaacgtt cacgcgcatt cgtcggtgcg ccctcgtgga
     2401 ttatttccgc atcatttgaa ctctcgcaag cggcagacgg atgcaattgg gtcgcataat
     2461 cggaaccata gtcgcaaccc agaaaataga acgcctctgt ggcgtgagac ttgtggttct
     2521 ggagccgctc aacgcgcagc gcgaaccgac agggccgcca atcgccgcaa tcgatgtagt
     2581 ggcggcggga gttggcgata cggtgttctg ggttgggtcg ttggaagctg cgcaggcgct
     2641 tgaaccgcca ttcccggcgg tcgacgcgag cgttgtgggg cttgtggaca gggtggatct
     2701 gctcccgggg aaacccgcat ccgaagaaac gggggcggaa tgattgtcgg acgggtggtg
     2761 gggagtgttg tttctacagt caagcacccg gcgtacgcct ccacaaaact gcttctggtg
     2821 aggcccgtga gcatggacgg ggtcgaagag ggcccggaga tggtgtgtgt tgataccgta
     2881 ggagcgggcg ccggggaact ggtactggtc tgcaaggagg gcgcagccgc gcgacaggtg
     2941 ctacggaaga agcacgcgcc cgtaaggtcg ctcgtgatag ggattgtcga tcacgtggaa
     3001 gccgcggacg gacggcgcat gatcgacgga agagattaga gatacgcaac agggacataa
     3061 ccatggcgat accgcttttt cagctcaaac aacagattgt ggatgcgtgc catcgcgtgt
     3121 acgccaaagg ttgggtcgcg tcgaacgacg gaaacctcag cgtacgggtc gacggtgaga
     3181 gggttctctg cacaccgaca ggaatcagca aagggctggt caccgtggat gacctgtgcc
     3241 tgatcgacat ggaaggcaac aagctcgaag gagcagcgca ccgtaagccg tcgacagaaa
     3301 tgaaagttca cctgatggcg tacaaggaac gtccggatgt ggtttccgtg gtccatgccc
     3361 acccgccgta cgcaaccggg tacgctctgg cgggactcga cctcggggag tgcaagcttc
     3421 cggaggtcgt cattgcgttg ggaaccatac ctttgaccaa gtacggattg ccgggaacgg
     3481 acgatctgcc gaacaaaatg cgcgagttca tccatcagtc ggacgccttc cttatggagc
     3541 tccacggggt gctgacggtg ggacagagtg tcctcaacgc gtatttcaag atggaaacgg
     3601 tggagcattt cgccaaaata gatttcatcg cccgccagct cggtggagcg cgtctgttct
     3661 ccaaggaaca ggccgaggaa cttctggccg cacgggcaag atacggcgtc acgacgccga
     3721 acatcgggtg tcgggctgcg gacgggtatg cttacccgcc cgatgcccgg gataaccttg
     3781 gaccgggtgc caatcccggc tctggcatcc ctgaggacaa gctcgtggaa gaggtcacga
     3841 agcgagtggt tcaggcgctc aaagccaaga actaagttgt ggcacgtgaa ccagatatgg
     3901 cgggccagtt tctgtacggc gggcgcacgg taatgcgccc gtttgatttt gcgacgggtt
     3961 ggagggaggc aggatgcatg ttctggtcgt aaacgttggc agcacgtcgt tcaagtaccg
     4021 cctcctggct atgccggaag agcgcgttct ggcagaaggt cgcttggaac ggatcggcag
     4081 cgcatcttct cccgtggaat tctcaagcgg cgagcattcg gaaaagagca cgatctatct
     4141 tccggactac gcggcagcca tcgggaaaat gatgtccgac ctgacctccg cgaagagccc
     4201 ggtcgaatcg ctgaaacaga ttgacgcggt ggggttcaag acggtggtga ttgacggcgt
     4261 tcccggctgc gtcctgctgg acgacgacgc cgtgagtcgt atggaagcct tgaacgacat
     4321 cgcccccgcg cacaatcccc cgtacgtgaa cgcggttcgc cagatgagag cgcttttccc
     4381 tgaccttccc ctggttgggc tgttcgaacc cgcatttcac gttacgatgc ctgattacgc
     4441 gtacacctac ggtgttccgc actcctgggc cgagaagtat ggtgttcgga aattcgggta
     4501 ccatggcgct tcccatcggt atgttgccga aacggctccc aagctcgcag gtatcgaccc
     4561 ctcccgctgt cgcctcattt cggcccatct cggggggtca tcttctctct gcgcaattcg
     4621 caacggccag tcgattgaca cgtccatggg catgtcccct cagagtgggg tggatcattc
     4681 cacgcggaac ggagacctcg atgtgttcgc agtgctccac gtgatggaca aagaaggctt
     4741 gagtaccagt gacgtgcgcc gtattctgtg caaagaggcg ggcctcgcgg gcatcagcgg
     4801 gacctccggt gatgtgcgtg atctcgaagc ggcggccgcc agtggaaacg accgtgcgcg
     4861 cctcgcactg gacgtgtttt gttacaacgc aaaaaaaacc atcggcgcga tggcggcggc
     4921 tctgggtggc ctggacgtga tagggctcgc gggcggcatc ggggagcgtg gcgtcaatgt
     4981 gagaaaacgc atactcaatg ggctcgattt cctcggagtg gccctggacg aggagaggaa
     5041 ctctgcgcac accgggcagg aaggggtgat ctccacagac gcctcaccgg tcaaagtcgt
     5101 cgtcgtccgc acgaacgagg agcttatcgt tgcgcggtct gtgtttgcac ttctgagcgg
     5161 gaacgcttcg tgagggcggt atggaactcg tctgggcgcc tgaaagaatc ggcgaagggc
     5221 gcaggttcag ggtaatcata cgatcggatg cacccgtacg gtgtttctat tccaaacacc
     5281 tcgaactggt tgatcgaacg cctgaggcgc acaactcact gcatttcttg tatttccgga
     5341 gcgtaattcc gaccaccgcg gcggaaatcc gatgcgaaag cggaagcgag tcaattgttg
     5401 tgcgattgcc cgtcattgcg gaagcagact gggagcacga cgccaggctt catgagatac
     5461 cgctgccccg catctggccg atggagagga cgtactttcc actgaaaaac cgccacacgc
     5521 tggttgacgg gaacgaaatc ggcgttcgga acagtgagac gcaggtttcg gtgaacgaag
     5581 gccacgccgt tgactccgcg ggaagcgcac gacgaaaaca gggtgacaga acgtcatgcg
     5641 aagcgctgga gtggcccgac gaagaaatct ggaatctcga accgcagtgc gacattccac
     5701 ggtggcactt tctaaacctc gacaaaggct gccctgtaca cggccttgcg atctactcgc
     5761 acaacgccta ttacccgtgg atcgtggacg aacggaaccg gccgtatcat gtgaaatgtc
     5821 cggttggtgg ggaatggtac cccaccaaca atctcgccga aggcgaccac accagtggtg
     5881 cctttccgga cgacgggtgg ggctggcaca gccccgacgg ggccgttttc ggcttcaccg
     5941 cctacgcgtt gctccgccgc atccgcctcg tgtacggcac cgtgcagtct ctcgcgcagc
     6001 atttcaaggg gacgggagac cagaaggcgg ccagaaagat cgtgtatctg ctgatcagta
     6061 tcgctcgtga acaccgttat ctggcgtttt ttccagaaca ccgcttccgc aaatacgagg
     6121 aggtagtgga agagccgcac taccgggaac acaaggaccg gatctcgtgg ggaccgcggg
     6181 agacaaaaac ggtggcaaac ctcgcaggtg gctccggcat ggacgagtat tgcatcaaca
     6241 tgctgtacca ctatgagcac ctctcccgtg catacgatat ggtgttcgac cgaattgacg
     6301 gcgattcaga gctgatagcg tttgtccggg ccagaatgcc ctcgctttcc gacggggaaa
     6361 agattcggcg gtacatcgaa acgttcctgt tccgggttgg cgtgcaggct gccctcgatg
     6421 atgcgcttat gtcgaatctg cctcgtcccc agatggcgat gctcgacttg atacgcctgc
     6481 tggatcagcc ggaatgcaag ggcctcgtgg actggctcgt ttcgggtggc gggcaggtgg
     6541 ggaggatgcc aacgaatttc tactacaaag acggcgcgtc atatgagtcg cttggcgggt
     6601 ataacggtat tcatgtatcc gcgttgattc cgattgtgtc cggcctgcgt gctcttcgga
     6661 atgagcaccc tgcgctttac tcctctccag cttacgaccc gattgccgga cacgaaagat
     6721 tctggcacgt tctgcggttc ccgtttgaat gcgtggttgc gcagaccggt tacccgctta
     6781 ttggcgattc cggcgatatc cccaacacga aggtgtgcag gacggttgtc tgcatggacg
     6841 taggggaccc gctggcggtg tatgacgaag ccgtcagatt gttccctgat gacacaacgt
     6901 tttcgaccat gcgcgagctg cttcgcaggc aggcttctgg cgaagcgatc gaaccggatg
     6961 cacggttaat gcagccttca cgacttctgg acgggtacgg gctcggcata ctggaatcgg
     7021 gggatctgga gaaccgccgc ggggtatggc tctattacgg cgatcacccg gggcattccc
     7081 atgaacagcc catggacatg ggactcttcg ctcacaaacg aaacatcctg aggcacatgg
     7141 ggtacccgta ctcgtggcag catatggata cgtgggacgg gaattggatc acccatttcg
     7201 gggtcaaggt catggcttca gagccacccg catggaagaa cagcatacac ttccttttcg
     7261 gaagcgggcc gtttcaggtg gtcgaggctt ccgggtacgg gctaggcaat cgagaaacgg
     7321 gagagtggga accattaccc gggtgcgtgc aacgaaggac actctgcctt ctcgacctcc
     7381 ccgacgggcg ttactatgcg gtggacctgt tccatgtgga ggggggcgac gaccactggt
     7441 ggactttcca cggacttccc ggggaattca cgatgagtgc agagcttccc gcgcaggaag
     7501 caggaacggt ggccggcaag gatgttgcct acgggcagac tcctcccgac ggcgttccga
     7561 aaagccttgc gtatctctac gacgtgttcc gcgggcagat cgaggaaccc tggtctgcgc
     7621 actggctgct
//
LOCUS       DAOSOO010000160         7620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9620_length_7620_cov_6.105882, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000160 DAOSOO010000000
VERSION     DAOSOO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_7620_cov_6.105882"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..763)
                     /locus_tag="PLT86_10705"
     CDS             complement(44..763)
                     /locus_tag="PLT86_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOT37122.1"
                     /translation="MNVDLSATWQEASRVTLRGRIAVVIDVLRATSTIVTAIQSGARE
                     VIPVETPEDARLAAQETGAYGAVLGGERGGLKIEGFDFGNSPYEYTPHSVGGKAVIMT
                     TTNGTRAFAAAREADITVAAALLNIGSVANVLLHEGRDVAVICAGTEGRVSLDDLYCA
                     GALIERIVTPGSDWVLNDGAKLAVHWYGSNTGTSFDVLASCAHGRRLAELGFHRDVEY
                     CSGMDTVTEVPVLKGRSLVSR"
     gene            complement(760..1905)
                     /gene="gcvT"
                     /locus_tag="PLT86_10710"
     CDS             complement(760..1905)
                     /gene="gcvT"
                     /locus_tag="PLT86_10710"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT37123.1"
                     /translation="MRFLMRNMVTTSPVPLLRTPLFNRHLALGARMAPFAGFEMPIMY
                     RSIAEEHNAVRHGCGIFDVSHMGRLRFSGTAGHELLERLTTIHVASVGAGTARYGFIL
                     TDSGGILDDVILFATRAAEWVLVVNAGNRRSVVEHMGKLEPHPRFFDDTESTGMVAVQ
                     GPATRAVLRQVMPRLAVPDRANRVIETDWNGTPVVLSTTGYTGEDGVEIIAASGRIEA
                     LWDSVIEAGAVPAGLGARDTLRLEMGYPLHGHELSKETDPWQAGLGWAVDRRNEKFTG
                     RSALLARGNDKTPRLVGLKAAGRGIPRAGNLVEDHGIVVGEVTSGTFSPSLQQGIALA
                     YVGFGAASPGKQFSVRDRESESRRITMEQVLAPFYRTGTRRSARNAS"
     gene            complement(1929..3614)
                     /locus_tag="PLT86_10715"
     CDS             complement(1929..3614)
                     /locus_tag="PLT86_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37124.1"
                     /translation="MRWFAVSLLCLLFHLQATAGDEFEPSDPDEASYQNARAEYAELD
                     SAAFGTDRSRTDRPFFGASRNTRHPRLALRSRLTGWDLAASTSPDNFTFNNRIMVRQR
                     GVVAGFITERDHDEPRMMDYRSGTLGFLFHGDSTRVVLGAMAAHVGSGLIVGRQGLNW
                     TGALSFRERETALTPALTTTESGALHGIGFRYVGRVWEILALYTTPRWDATIDSATNS
                     ADEISVTGLHRTDAELRAAGALKETFRLFRIGGNFGRSARVGLTAAKSKFSPRTLYRH
                     SSKATDEISFLGVDAQSTIGSFGVRGEAVLQDEREYGGAGALRFHNESLTMTAAGWLY
                     SSAFTSWHGTGWSLHSEASSERGGALVVEWAPGSGVHVDGFLAHSVEPLDAAGRDFPR
                     WTTVEELRWRTPLSTRFSWIGRWKQKSTRSTVAEGIFMERRRDISSDLELIASGMTFR
                     GGWAFAQNQSGDRGGLVRIRMQSRPARGLDCTLGGAFHRTNSTSLPLYFYEQQVPNYG
                     FLWSLYGSRASWTVRARYHTGRATASLFVLGTGTLNEAADRLTVTAQIDYVLR"
     gene            complement(3672..4697)
                     /gene="larE"
                     /locus_tag="PLT86_10720"
     CDS             complement(3672..4697)
                     /gene="larE"
                     /locus_tag="PLT86_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOT37125.1"
                     /translation="MTLEEKHARLREMLLEAAPVVVAFSGGVDSTFLLAAAHRALGKA
                     KCIGVTAVSESLAPAELDEARQIANQIGAEWTTIQTREMDNPRYRANTNERCYFCKTE
                     LFSTLQTLARERGFATVLDGFNADDTGDWRPGARAGVEQGVKSPLKDAGLTKQDIRDL
                     SRSYGLPTWNKPSFACLASRLPYNTEITPERLSLIASAEAVLRSHGFRAFRARYHGET
                     ARLEIGIDEIPRFQEAMLAQSVRDAVMKAGFAGVTLDLAGFRSGSQNETGGSGIVPLP
                     SAARDGLSIIKKQGFAVAKTRIEGNMIRIIVSWEDFPRILKEETRIPLVAALRGQGYV
                     YVAADIP"
     gene            complement(4712..6589)
                     /locus_tag="PLT86_10725"
     CDS             complement(4712..6589)
                     /locus_tag="PLT86_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT37126.1"
                     /translation="MIQSARPLLVKVSVFVAGFLPLALVFGAQISNAAIPERRNQAPL
                     PVERPDSVFALALQDYDDGRFVEARWAFESLTNNLPRSSPLRPASQLMWARSLYRLGD
                     YRAAESAVLPLAQETGSGAPNPAFAAHGQYLLGLVWWRTGRYADAIRNLHAVSSSPVV
                     SETTKRDARLLSLASVARVEQVNFAEFDTSQAAFVRDALQLNRAIALFNASQMTAARL
                     VLEQLQRISPDTPFASEVAALLADVKSYERTEIRLAVVTPLSGEDSTSGAALLDGVKF
                     AFERLAPPGLSPLVTKSAATQVETIHAVEGFARDTTVLAVIGPLTTENSIVAGAVANA
                     MGLPLVTPTASGEGVCGIGDNVFQINLTARAQGAFLASVVADSLRAQTVAILSTYDPH
                     DQALSSEFARVVREKGLIVIAHEHFITGTTDLTRPLSAIRRAALQRTPPPADMDTVHF
                     MPEINSIDAMLVCSSHEADLVAVAAQIPTQKIWTRVVGNATWGNSSVRQQAGEAAEGV
                     LFATTFDQSYPEARNFAEAYRAERGNEPILATALGYDATSLVLQAISTGARTRPEVRR
                     FLADVKEWPGVTGLITLAPDGCNRNAMVRMIRGGTIRPVGDWQILSRAATQNATPQVG
                     Q"
     gene            complement(6654..7181)
                     /locus_tag="PLT86_10730"
     CDS             complement(6654..7181)
                     /locus_tag="PLT86_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT37127.1"
                     /translation="MAMVQVVVHGLGLDVNHGPVVLLKEAEGERVLPIWIGHPEAHSI
                     QMKLENQDCGRPLTHDLMKSVMDLLEARLLRVEITKLQDSTYYAELVVRTDAGEARID
                     ARPSDSIALALRFDAPIWAEDGLFRSPETEQSDTESGQPEETDEQRAERQKTELQRRL
                     RHIDPSSFGYYRLGD"
     gene            complement(7234..>7620)
                     /locus_tag="PLT86_10735"
     CDS             complement(7234..>7620)
                     /locus_tag="PLT86_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37128.1"
                     /translation="RDADYDADSILSARNGNLLFRLREAFRGGGPDRLLVVTGDRAFG
                     KRDRTDALSREITEMINDARNVLFHVPRASELHRRDAERGMTRGLIHGFSLLGRDSVE
                     KRELRVEMVAAQRDVIDRFRRPAYID"
BASE COUNT         1523 a         2411 c         2163 g         1523 t
ORIGIN      
        1 agccgtgggc gccatttgca cgtgccaatc ccgccctcct cgtttatcgg ctgaccaggg
       61 agcgaccttt cagcacaggt acttctgtca ccgtgtccat accggagcag tactccacat
      121 cgcgatggaa tccgagctcc gccagtcgtc gtccatgcgc gcaggaagca aggacatcaa
      181 agctggtgcc ggtattggaa ccataccagt gaacggccag cttcgcgccg tcgttcagca
      241 cccaatcgct tcccggggtg acgatccttt cgataagcgc gcccgcgcaa tacaggtcgt
      301 ccagggatac cctcccttcc gtacccgcgc aaataacagc cacgtcacgt ccttcgtgca
      361 gcaggacatt tgcgacgctc ccgatattca gcaaggccgc cgcaaccgta atatctgctt
      421 ctcgagccgc ggcgaaagct cgcgtgccgt tcgtcgtagt catgatgacg gccttgccgc
      481 cgacggagtg cggcgtgtac tcgtaaggcg aattgccgaa atcaaaccct tcgatcttca
      541 ggcccccgcg ttcgcccccc agtacagctc cgtatgctcc tgtttcttgt gctgccaacc
      601 gcgcatcttc gggagtttcc accgggatca cctcgcgggc gccggattga atcgcggtga
      661 caatggtgct cgtcgcgcgc aggacatcaa tcaccacagc gatacgaccg cgcagcgtaa
      721 ccctggatgc ttcctgccag gttgccgaaa gatcgacgtt catgatgcgt tcctggcgct
      781 tctgcgggtg ccagtccggt aaaacggcgc gagaacctgt tccatcgtga tccgtcttga
      841 ttcggattcg cggtcgcgga ccgaaaactg ttttcccgga cttgccgcgc cgaagccgac
      901 gtacgccagc gcaatgccct gctgcagcga aggcgaaaag gtcccagagg tgacttcccc
      961 cactacaatt ccgtggtctt ccaccagatt ccccgcgcga ggtatgccgc gtcctgccgc
     1021 cttgagaccc actaggcggg gtgtcttgtc attcccacgt gccaacaacg cggatcggcc
     1081 cgtaaatttc tcgttcctcc ggtcaacggc ccaccccaga cctgcctgcc acggatcagt
     1141 ttccttcgaa agttcatggc cgtggagcgg gtatcccatc tcgagacgca gcgtatcacg
     1201 cgcgccaagc ccagccggca cagctccggc ttcgatcacg gagtcccaca gggcctcaat
     1261 acgcccgctc gccgcgatga tctcgactcc gtcttcgcct gtgtacccgg ttgttgagag
     1321 gacaacgggc gtgccgttcc aatccgtttc aatcacacgg ttcgcgcggt caggtaccgc
     1381 aagccgcggc attacctgcc tcagaaccgc ccgtgttgct ggcccctgaa ctgccaccat
     1441 gcccgttgat tcggtatcgt cgaaaaaacg gggatggggt tccagcttcc ccatatgctc
     1501 aaccacggaa cgccggtttc ctgcgttcac gacaaggacc cactccgctg cgcgcgtggc
     1561 gaaaagaatc acatcgtcaa ggatcccgcc gctgtcggtc agtatgaacc cgtaccgcgc
     1621 ggtgccggca cctacggaag caacgtggat agttgtcagg cgctcgagaa gttcgtgtcc
     1681 cgccgttcca ctgaaacgca gcctgcccat gtgggacacg tcaaatatgc cacagccgtg
     1741 ccggactgca ttgtgttcct ccgcgatact ccgatacatg atcggcattt cgaatccggc
     1801 gaacggagcc atccttgcgc cgagtgcgag gtggcggttg aaaaggggtg ttcgcaacag
     1861 gggcacggga gaagtcgtca ccatgttcct catcaaaaag cgcattcacg catcccgaac
     1921 aatttcgatc atcgtagaac ataatcaatc tgcgcagtca cagtcagccg gtctgccgct
     1981 tcgttcaagg tccccgtacc cagtacgaac agcgatgcgg tggccctgcc cgtgtggtac
     2041 cgggcacgaa ccgtccagct tgcgcgggat ccgtaaaggc tccagagaaa accgtagttc
     2101 ggcacctgtt gctcatagaa gtacaacggc aacgaggtcg agtttgtgcg gtggaaggcg
     2161 ccgcccagag tgcagtccag tccgcgcgcc ggtcgacttt gcatccgtat ccgcacaagt
     2221 cctccccggt ctcctgattg attttgggca aacgcccacc cgcctcggaa agtcatccct
     2281 gaagcgatca gctcaagatc agaagagatg tcccgccttc gctccatgaa tatcccctct
     2341 gccacagtag accgcgtcga cttctgtttc caccttccga tccacgaaaa tcgcgttgaa
     2401 agaggggttc gccagcgaag ttcctcgaca gttgtccacc gaggaaaatc gcggcctgcc
     2461 gcatcaagcg gctccacgga atgcgcgagg aatccgtcca cgtggacgcc cgacccggga
     2521 gcccattcaa ccacgagtgc accaccacgc tctgaggaag cctcgctgtg gaggctccat
     2581 cccgttccat gccacgaggt aaacgccgac gagtacaacc aaccggcggc agtcatggtt
     2641 aacgactcgt tgtgaaaacg aagcgcaccg gcgcccccgt attcgcgctc atcctgcagg
     2701 accgcttcac cccggacgcc gaaactcccg attgttgact gagcgtcgac accgaggaaa
     2761 gagatctcgt ccgttgcttt cgaggaatgg cgatacagcg tgcgggggct gaacttgctt
     2821 ttggctgctg tcagtcccac gcgcgccgac cgcccgaaat tgcccccgat tcggaagaga
     2881 cggaacgtct ccttgagtgc tcccgcggca cgcaattctg cgtccgtccg gtgcagacct
     2941 gtgacactga tttcgtcggc gctgttcgtt gcgctgtcga tagtcgcatc ccacctcggc
     3001 gttgtgtaca gggcgaggat ttcccataca cggccaacgt agcgaaagcc aattccgtgg
     3061 agtgctccgg attctgtggt agtcagggcg ggagtgagtg cggtttcgcg ttcacggaac
     3121 gagagcgcgc ctgtccagtt gagtccctgt ctgccgacga tcagccctga gccgacgtgt
     3181 gccgccatgg caccgagtac gacgcgcgtg gaatctccgt ggaataagaa accgagtgtt
     3241 ccgcttcggt aatccatcat ccttggttcg tcgtgatccc tctcggtgat gaacccggcg
     3301 acaacacctc tctgccgcac cataatcctg ttgttgaagg tgaagttgtc aggagatgtg
     3361 cttgcggcca ggtcccaacc cgtcagtcgg gaccgtaaag ccaggcgggg atgcctcgtg
     3421 ttgcgcgaag caccgaagaa cggcctgtcg gttcgggatc ggtccgtgcc aaaggctgct
     3481 gaatcgagtt ccgcgtactc cgcccgggcg ttctgatacg aagcttcgtc agggtcggac
     3541 ggttcgaatt catcaccggc tgtcgcctgc agatggaaca acaggcagag cagactcacg
     3601 gcgaaccacc tcacaccttc ctcctcaccg gcaggtggct tcgggacgcg gagcgtttcg
     3661 gtgacgaata cctaagggat gtctgctgcg acgtacacgt acccctgtcc gcgcagcgca
     3721 gccacgaggg ggatgcgagt ctcctctttg agaattcggg ggaagtcctc ccacgaaacg
     3781 atgatacgta tcatgttccc ttcgatacgt gtcttcgcaa cggcaaaccc ctgtttcttg
     3841 atgatcgaaa gcccatcgcg ggcagcagac ggaagcggga caatgccact gccgcctgtc
     3901 tcgttctggc ttccggaccg gaaacccgcg aggtcaagag tcacaccagc aaaacctgcc
     3961 ttcatgacgg catctcgaac ggattgcgca agcatagctt cttggaatcg tggtatctcg
     4021 tcaattccta tctcaagccg cgcagtctcc ccatggtacc gcgcccgaaa cgctcgaaaa
     4081 ccgtgtgagc gcagaactgc ttctgcggaa gcaatgaggg aaagcctctc cggggtgatt
     4141 tccgtgttgt acggcaggcg ggaggcaaga cacgcaaacg atggtttgtt ccaggttggg
     4201 agaccgtaag accgcgagag atcgcggatg tcctgtttcg tgaggcctgc atccttgagc
     4261 gggctcttga ctccttgttc cacccccgcg cgcgcacctg gcctccagtc cccggtgtcg
     4321 tcagcgttga acccgtcaag aacagtcgca aaacctcgtt cgcgcgcgag ggtctgcaga
     4381 gtgctgaaca gctccgtctt gcaaaaatag caccgctcgt tggtgttggc gcggtaacga
     4441 gggttgtcca tctctctcgt ctggatcgtc gtccattcgg ctccgatctg gttcgcgatc
     4501 tgccgtgctt cgtccagctc cgctggagcc agcgattcag acaccgcagt taccccgatg
     4561 catttcgcct tgccaagcgc tctgtgcgcg gccgcaagga ggaacgtact gtccacgccg
     4621 ccggaaaagg ctaccacaac cggtgcggcc tcgaggagca tttccctgag ccgtgcatgt
     4681 ttctcttcca gggtcatcag gcttcgctcc tttattggcc aacctgaggc gtggcattct
     4741 gtgtcgccgc tcgggaaagg atctgccagt cccccacggg ccgaatcgtc ccgccgcgaa
     4801 tcattcggac catggcgttc cggttgcacc cgtccggcgc cagggtgatg agtcccgtaa
     4861 cgccgggcca ttctttcacg tctgcaagga aacgacgaac ctcgggccgg gtcctcgcgc
     4921 cggtagatat tgcctgcagc acaagcgacg tggcgtcgta tccaagcgcc gtcgcaagta
     4981 tcggctcatt tcctcgttcc gcgcggtacg cctctgcgaa attacgagct tccggatacg
     5041 actggtcaaa cgtcgttgcg aacaggaccc cctccgcagc ctcaccggcc tgctggcgca
     5101 cggaggagtt gccccacgtg gcgtttccca caacccgcgt ccagatcttc tgagtgggaa
     5161 tctgcgccgc taccgcaacc aggtctgctt cgtgcgaaga acagaccagc atggcgtcaa
     5221 tcgagttgat ttccggcatg aagtgcaccg tgtccatgtc ggcgggggga ggagtccgct
     5281 gtaacgccgc ccggcggatc gcgctcagcg gacgggtaag atcggtagta cccgtaatga
     5341 agtgctcgtg cgcaataaca atcagacctt tctcgcgaac cacgcgggcg aattccgagg
     5401 aaagagcctg atcgtgcggg tcgtacgtac tcagaatggc aacagtctgc gcccggagac
     5461 tgtccgcaac caccgacgcg agaaatgccc cttgcgcgcg cgccgtcaga ttgatctgaa
     5521 agacgttatc accaatccca caaacgccct cccccgaagc cgtcggcgta acaagtggga
     5581 gacccatcgc gttagccact gcgcccgcga cgatggagtt ctccgtcgtg agaggaccaa
     5641 tcaccgccag caccgttgtg tcacgcgcaa aaccctccac ggcgtgtatg gtctccactt
     5701 gcgtcgctgc gctcttggtc acgaggggcg aaagaccggg cggcgcgagt cgctcaaagg
     5761 caaacttcac tccgtcaaga agcgctgcgc cggatgtaga atcctcccct gagagcggcg
     5821 taaccacagc caggcggatt tccgttcgct cgtagctttt gacgtccgcc agcagcgcgg
     5881 cgacttcact ggcgaaagga gtgtccggag aaatcctctg cagttgctcc aggaccagac
     5941 gcgcggcagt catctgggat gcgttgaaca gggcgatcgc acggttgagt tggagggcgt
     6001 cgcgaacgaa cgcggcctgg gacgtatcaa actccgcgaa gttgacttgc tcgacccgcg
     6061 cgacggaagc cagactcaga agccgcgcgt cccgttttgt tgtctctgag acgactggtg
     6121 aactggaaac cgcgtggagg tttcgaatgg catccgcata gcgccccgtc cgccaccaga
     6181 ccagccccag caggtattgt ccgtgcgcgg caaaagcggg attaggtgcc ccgcttcccg
     6241 tttcttgagc gagcggaaga acagccgatt cggccgctcg gtagtctccg agccgataaa
     6301 gagaccttgc ccacatgagc tgacttgccg gtctcaacgg agagcttcgg ggaagattgt
     6361 tcgtgagact ttcgaatgcc catctcgctt cgacaaaacg cccgtcgtcg tagtcttgaa
     6421 gcgccagcgc gaaaaccgag tccgggcgct ccacgggcag gggagcctgg ttcctccgct
     6481 ccgggatggc agcattgctg atctgcgcgc cgaaaaccag cgcaaggggg aggaaacccg
     6541 caacaaagac agaaaccttc acgagcagag gtcgagcgga ctggatcatt aagagacctt
     6601 ctcaagagga ttccgggtaa aggtcaaagg tttgcgcaag gagaccgcgc cactcagtcc
     6661 ccgaggcggt agtacccgaa actgctggga tctatgtgcc ggagccttcg ttggagttct
     6721 gttttctgcc tctcggcacg ctgttcgtcg gtttcttctg gttgtcctga ctcggtatcg
     6781 ctctgctcgg tttccggcga ccggaacagg ccatcctccg cccatattgg tgcgtcaaaa
     6841 cgcaatgcca gcgcgatcga gtcgctggga cgtgcatcga tccgcgcctc tcccgcatcc
     6901 gtgcgaacaa ccaactcagc gtaatacgta gaatcctgaa gctttgtgat ttctacacgt
     6961 aaaagacggg cttccagcaa atccatgacc gatttcatca gatcatgtgt cagagggcgg
     7021 ccgcagtcct ggttctccag tttcatctgg atggaatgcg cctctggatg ccctatccag
     7081 atcgggagca cacgttcccc ctccgcttcc ttcagcagaa cgaccggccc gtggttcacg
     7141 tccaaaccga ggccgtggac taccacctga accatcgcca tggacgtctc cctgtgcgtt
     7201 cttgcgacca attcctacat ttttacaggt ttgttagtca atataggccg gccggcgaaa
     7261 ccggtcaata acatcccttt gtgccgcaac catctcgacc cgcaattcgc gtttctcaac
     7321 cgaatcgcgc cccagcaggg agaagccgtg aatcagaccc ctcgtcattc cccgttccgc
     7381 gtcgcgtctg tgcagttccg aagctctggg cacgtggaac agaacgttgc gcgcatcgtt
     7441 gatcatctcc gtgatctctc gcgacaaggc gtctgtgcgg tcgcgtttcc cgaatgcgcg
     7501 gtcaccagtt acgacgagaa ggcgatccgg gccaccgccc cggaacgcct ctcgcaggcg
     7561 gaaaaggaga ttgccgttgc gtgcagagag aatggaatcg gcgtcgtagt cggcatccct
//
LOCUS       DAOSOO010000161         7488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_9869_length_7488_cov_5.457420, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000161 DAOSOO010000000
VERSION     DAOSOO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9869_length_7488_cov_5.457420"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..864)
                     /locus_tag="PLT86_10740"
     CDS             complement(100..864)
                     /locus_tag="PLT86_10740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOT37129.1"
                     /translation="MIEITPRDAARARGNTKRRYVTAMFDRIAPRYDAMNIVISLGQT
                     SIWRRRALKGISLPHRAVILDVGCGTGSVIRLLKRRFRSARYLGIDLSSRMLEKASLR
                     EPESDFCVGDAGSIPCRTETCDLITTFFTTRNFSDLPAAVREMMRVLKPGGRLVVLDS
                     FPASGSPLWRVLKKGWMYVAVPFLVRPFTDAQAYRYLAESIEAHCSAEALVDLLTSCG
                     AESPSIRFLSFGAAAVISASKSTEAGHGRPSSEQDE"
     gene            892..1716
                     /locus_tag="PLT86_10745"
     CDS             892..1716
                     /locus_tag="PLT86_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT37130.1"
                     /translation="MPVSVAGPGVILNFRVSPCTKEPALSHSDIITVLAAFAIGLSKT
                     GFGGGLGAIVSPMFVIALPPREGIGLMLPLLIVGDMLTLTFYWRGWDRSNLVALFPGV
                     VVGIGLGMILLGHIPDNEFRVVIGLLAMVFGSGQALRQWLVPHAEAFTGNRFLGALAG
                     FVTGTVSAIAHQGGLVTTLYLLPQRLTNQAFVATSGIVFTLTNLTKLPAYVYAGLVTW
                     PIIVKAATLSPAVILGALLGVRLNRQVPQKHFAWIVLFFVLVTGAKLVWDYFMSTG"
     gene            complement(1717..2733)
                     /gene="rsgA"
                     /locus_tag="PLT86_10750"
     CDS             complement(1717..2733)
                     /gene="rsgA"
                     /locus_tag="PLT86_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT37131.1"
                     /translation="MIFAQSIVATSMCEASFVGTVIRKHRNFYVVERDATEVLCSLSS
                     KLRKNLEYPEADISSRRRRVQAVHKVGFVDPVVVGDDVRVEPGAGIGLIVERLERRSV
                     LSREAPSRTHLQQVIAANVDQVLAVVSPQSPPFRPDLLDRLLAGCEHHNLKGIVCLTK
                     IDLGVPGEVERVIETYPGIGYPLVRTSAVTGEGLQNLEQLLAGRNTVAVGLSGTGKSS
                     LVNALVPGANLPVGHVNRKTGLGTHRTTTISLHRLASGGHLVDAPGLRELGLWDCSAD
                     QLPDLFPEFRNLVDACRFRGCAHRDEPGCAVKTAVEENAIAHHRYRNYLSIRAELERR
                     EQQG"
     gene            complement(2730..3998)
                     /gene="murA"
                     /locus_tag="PLT86_10755"
     CDS             complement(2730..3998)
                     /gene="murA"
                     /locus_tag="PLT86_10755"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT37132.1"
                     /translation="MDAFRIQGGTRLAGSVEIEGMKNAILPMMAASILATEGTLVLEN
                     VPQLRDVTVMLRILETLGVKGGYDPQKRTLSLDATGVIGDTAPYDLVRQMRASFLVSG
                     ALVARLGRCRVSMPGGCAIGSRPVSDQLRAFKALGGVVNEIKGYVEVEARNRSGRLVT
                     LDYPAATGTENIVLLACLTEGTTTIENAACDPEVVDFGECLVKMGAQITGLGTNTVRV
                     TGVKALRGTRHRVVPDRIDAATFAVGAAMTRGDVFLANARSDHFEIVLTKLEEAGAIV
                     TRLDTGVRVQGPDRLKSVNVRTLPYPAFPTDVQAPMMAAMATAEGASLIWEQVYDNRF
                     TQGPELRRMGANITIAGDKAVVVGVPELTGAPVMASDIRAGGSLVLAGLVAKGETTVS
                     RVYHIDRGYDHLETRLQKLGAVIERYNEPA"
     gene            complement(4217..4654)
                     /locus_tag="PLT86_10760"
     CDS             complement(4217..4654)
                     /locus_tag="PLT86_10760"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT37133.1"
                     /translation="MERQVRVLFLGTGNACRSQMAEAWAKTLKSHEMEVFSAGIEKQG
                     LSRRAVRVMAEAGVDISAYRSKTVGELPNRDFDFVITLSNAAHVNCPRFPGNPKINCN
                     VFDDPPALAEGSRDDAEALSHYRRVRDEIRAFVETLPEALQTL"
     gene            complement(4802..5011)
                     /locus_tag="PLT86_10765"
                     /pseudo
     CDS             complement(4802..5011)
                     /locus_tag="PLT86_10765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(5248..5324)
                     /locus_tag="PLT86_10770"
     tRNA            complement(5248..5324)
                     /locus_tag="PLT86_10770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5288..5290),aa:Val,seq:gac)
     gene            5478..6650
                     /locus_tag="PLT86_10775"
     CDS             5478..6650
                     /locus_tag="PLT86_10775"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOT37134.1"
                     /translation="MRDSRRTLRSGIVLPWTIVLGFLAVAMCLATGIRATDNGREGTR
                     PDATNSVSAQRLSRVPLQSDSRSDSFRVRFRTEFAKSLKNSGFAGDVKPRNRGATGDV
                     LVWDVRVPVGYSVLRATAEVISVVHSFGGNVYRAEHEYADPLKVKLAVGAAHAVTDSI
                     LLATDRSIASRGLLAIVIDDMGYRSMAESGRFADLPFPVNLAFLPAGENTLELAKRAR
                     ATRKTILLHLPMEPENGNEPLEPNTIMTAMSDEEIRELTRRHLESIPGLAGVNNHMGS
                     RATRDERVMRAVLAEVRDRNLFFLDSYTSPGSTAAATARSLGIPTLERDVFLDNSSDI
                     QTIQRELWAAADKASRSGRAVAIGHDRPETYEALRVTIPAIQRSGVRVCFLWDLLR"
     gene            6623..>7488
                     /locus_tag="PLT86_10780"
     CDS             6623..>7488
                     /locus_tag="PLT86_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT37135.1"
                     /translation="MFSVGLAPLKRVLAAIAVACSTGVLGCAAFAPRPAHVTAEHLDP
                     LQAFTVLKESPRTYKVRVGSLRRYRVSAPFVGQEPVQVYDLPAELGELWDEATSEALY
                     GNPHRSAQMLEAIVNAEPACAECRHEYARVLINLANKEKLTKERAHELRYRALDEMTI
                     AGVLDRECPSGAGAFYPPTSAGRTDIPLLPRTRQAGELFAAGNDAFNSGEFTQARSLY
                     GVVVQREPTFGRGYVGLGDVFVELGAYQEAAEYFTRALSVDSTDFLAWRSLAECAFAS
                     GNAELAKEAAVRA"
BASE COUNT         1483 a         2243 c         2161 g         1601 t
ORIGIN      
        1 ttccgatagg tgcgctcagt tgttcacgca gaaacgagaa cgcctcatcc gagacgatcg
       61 tgtcttggag gcagggtttc cgggaagaac ctgggccaat cattcatcct gctcagagct
      121 cgggcggccg tggccggcct ctgttgactt ggacgctgat ataactgctg ccgcaccgaa
      181 gctgaggaat cgaatcgaag gtgattccgc gccgcaagaa gtgagaaggt ccacgagcgc
      241 ttcggcactg cagtgggctt cgatactctc agcaaggtaa cgataggcct gtgcgtcggt
      301 gaaaggccga accaggaacg gaacggcaac atacatccag cctttcttca gaacgcgcca
      361 tagagggctg ccagacgcag gaaaactgtc gagaacgacc aacctgcctc ccggttttag
      421 aactcgcatc atttcacgaa cggcggcggg caggtcggaa aaattccttg tcgtgaagaa
      481 tgtcgtgatg agatcgcacg tctccgtcct gcacggaatg gaccccgcat cgccgacaca
      541 gaaatcgctt tccggttccc gaagcgatgc tttttcgagc atccgggagg aaagatcgat
      601 acccagatac cgcgcagagc gaaatctacg tttgagcagt cgaataaccg acccggtgcc
      661 gcagccgaca tcaagaataa cagcccggtg tggcaacgag attcctttga gagcacgtct
      721 gcgccagatg ctcgtctgcc cgagcgaaat cacgatgttc atcgcgtcgt aacgtggcgc
      781 tatcctgtcg aacatcgcgg taacataccg ccgcttcgtg ttccccctcg cacgtgccgc
      841 atcccgcggg gtaatctcaa tcacaccctg ttctccacgg ttgcacccgg cgtgcctgtg
      901 tccgtcgccg gacccggcgt aatattgaac ttccgcgttt caccctgcac caaggagcct
      961 gccctgtcac attccgacat catcactgtc ctggcggcgt ttgccatcgg actctcaaaa
     1021 accggtttcg gtggcggtct gggagcaatc gtgtcaccga tgttcgtcat tgccctgccg
     1081 ccgcgcgagg gaatcggcct gatgttgcca ctcctcatcg tcggtgacat gctcacgctc
     1141 accttttact ggcgtggatg ggacagaagc aatctcgtgg cgctgtttcc cggcgttgtg
     1201 gtcggcatcg gtctgggaat gatcttgctg ggacatatcc cggacaatga gttccgcgtc
     1261 gtgattggcc tcctcgcgat ggtgttcggc agcggtcagg ctctccggca atggcttgtt
     1321 ccccacgcgg aagcgttcac gggaaaccgt tttctcggtg cactggccgg atttgtcacc
     1381 gggacggtct ccgcgattgc acaccaggga gggcttgtca caacgctgta ccttttgccg
     1441 caaagactga ccaaccaggc cttcgtggcc accagcggga tcgtgttcac cctgacaaat
     1501 ctgacgaagc tgcccgcgta cgtgtatgcg ggccttgtaa cgtggccgat catcgtcaaa
     1561 gcggcaacgt tatcgcctgc cgtaattctg ggcgcgctcc ttggcgtacg ccttaaccgg
     1621 caggttccac agaagcattt cgcatggata gtgctgttct tcgttctcgt taccggcgca
     1681 aagctcgtgt gggattactt catgtcgacc ggttgatcat ccctgctgtt cgcgccgctc
     1741 cagctccgcg cggatggaca ggtaattccg ataacggtgg tgggcgatcg cattttcttc
     1801 caccgcggtc tttacagcac aaccaggctc atccctgtgt gcgcatccac ggaatcggca
     1861 cgcatccaca agatttctga attcaggaaa cagatctgga agctgatccg cggaacaatc
     1921 ccacaggcca agttcccgaa gccctggggc atccaccaga tgccctcccg atgctaatcg
     1981 gtgcaatgaa atggtggtgg ttctgtgagt gccgaggccc gttttcctgt tcacgtgtcc
     2041 taccgggaga ttggctcccg gaaccagcgc attcacgagc gacgatttcc cggttcctga
     2101 aagacccacc gcaacggtgt ttcttcccgc aagcagttgc tccagattct gcaatccctc
     2161 gcccgtcact gcgctcgttc gaacgagggg gtatccgatt ccaggatatg tctcaatcac
     2221 gcgttccact tctccgggaa caccgagatc gattttcgtc aggcacacga tgcccttcag
     2281 gttgtggtgc tcgcacccgg ccagcaaacg gtccagaagg tccggacgga aagggggaga
     2341 ttgcggactc accactgcga gaacttggtc cacgttggcc gcgattacct gctggagatg
     2401 cgttcgcgaa ggggcttccc gcgaaaggac tgaccgccgt tccagtcgct cgactatgag
     2461 accgataccc gcgcccggct ccacgcgcac atcgtcgccg acaaccacag gatccacgaa
     2521 gcctactttg tgcacggcct gcactcgcct gcggcgggaa ctgatgtcgg cttcggggta
     2581 ttcgaggttt ttcctcagtt tgcttgacag gctgcacagg acttccgtcg cgtctctttc
     2641 gacgacgtag aagtttctgt gcttccgaat cactgtgccg acaaacgagg cctcacacat
     2701 ggacgtcgcc acaatcgatt gcgcgaatat cacgccggct cgttgtaccg ctcaatcacc
     2761 gcccccagtt tctgcaaccg cgtttcgaga tgatcatacc cgcggtcgat gtgatacacg
     2821 cgagataccg tggtttcgcc tttggcaacc agtcccgcga gcaccaacga tccgcccgcc
     2881 cgaatgtcac tcgccatcac gggagcaccg gtgagttcgg gaacacctac caccaccgcc
     2941 ttgtcgcccg cgatggtgat gtttgcgccc atgcggcgta gttccggtcc ctgagtgaac
     3001 ctgttatcgt acacctgttc ccagataagg cttgccccct ccgccgttgc catggccgcc
     3061 atcatcggtg cctgcacgtc agtggggaac gcggggtagg gaagtgtacg cacgttgaca
     3121 gatttgaggc ggtcgggccc ttgaaccctg acaccggtgt ccagtcttgt gacgatggcg
     3181 ccagcttctt ccagctttgt aagcacgatc tcgaagtgat ccgaccttgc attggccaga
     3241 aacacgtcac cccgcgtcat ggccgccccc acggcgaacg tggctgcgtc aatgcgatcc
     3301 ggaacaaccc ggtgccgcgt cccacgtaga gctttgacac cagtcacccg cacggtgttc
     3361 gtcccgaggc ccgtgatctg agctcccatt ttgacaaggc actctccaaa atccaccact
     3421 tcggggtcgc atgccgcgtt ctcgatggtg gtcgttcctt ctgtcaggca cgccagcagg
     3481 actatgttct cagttccggt ggccgcaggg taatccagcg taacgagcct gccggaacgg
     3541 tttcgtgctt caacctccac gtatcctttg atttcattga ccacgccgcc gagcgctttg
     3601 aacgcgcgaa gttggtcgct tacggggcgg gaaccgatgg cgcagccgcc gggcatcgaa
     3661 acacggcacc gcccgagacg cgcaaccaag gcccccgaca cgaggaacga cgcgcgcatt
     3721 tgcctgacaa ggtcgtaggg ggcggtgtcg ccgatgactc ctgtggcgtc gagcgaaagg
     3781 gtccttttct gagggtcgta ccctcccttc acgccgagtg tttcgagaat gcgcagcatc
     3841 accgtgacat cgcgcagttg gggcacgttt tcaagtacca gagtgccttc ggtcgccagt
     3901 atggacgctg ccatcatcgg gagtatggcg tttttcatgc cttcgatttc cacggagcca
     3961 gccagcctcg tgccaccttg aatacgaaat gcgtccatgc agatttcctg gttgtgaacg
     4021 agaccgtgca gagcgctcgg cgccggtccg tgggaactga aatacgttaa ttcacgtggt
     4081 cacagcgaaa gtcgcgcagt ctacccttga acatgcgggc cttatcgcgg tgacacgccg
     4141 tccttgattt ctgtcatctt tgcgatcggc tccgcgatgc tgaacaccaa aacgtgacgc
     4201 gcgcccgatt gctgattcac aaagtctgca gcgcttccgg aagtgtctca acgaatgccc
     4261 gtatctcgtc gcggacgcgc cggtagtgcg agagagcttc cgcatcatcg cgtgatccct
     4321 ccgcaagggc gggaggatcg tcgaatacat tgcagttgat tttcgggttt cccgggaacc
     4381 tcgggcagtt gacgtgcgct gcgttgctca gtgtgatcac gaagtcgaag tcacgattgg
     4441 gtaattcgcc gaccgttttc gacctgtatg cgctgatatc cacccccgcc tcagccatca
     4501 cccgtacggc gcgacgactg agtccctgtt tttctattcc agcggagaac acttccatct
     4561 cgtggctctt gagtgttttt gcccaggctt ccgccatctg actgcggcag gcgtttcctg
     4621 ttccgagaaa caagacccga acctgccgtt caatctgcgc aggggacttc tgcgctttcc
     4681 gttcatcgcc ggatgcgtgg gtcagtttcg cgacatcctc gacgataacg gggtgctcgg
     4741 ccatctccgt ccgcaaaacc tgttgcagcg atcgctccag caagttcttc ggctccgcca
     4801 gacggtagaa catccacgtc ccgtccctcc gcacttcgac cagccgcgcc gaacgtaaca
     4861 ccgaaagatg acgggatatc ttgaaatctg cctcaccaag ggcttccgca agatcgccga
     4921 cacacgtctc gccccgcacc gcgaggagga tcgcgacgcg aaggcggata ggatccgcga
     4981 gagccttgaa gactcgcgcc tcttctttca tgggagtttc cgaacgcgaa cgtctgccgc
     5041 gaccatcctt tttccgaaaa gtaaagatag cggttaccgg tcaacgttca tcgcccgggc
     5101 ctcaagaaca acccaagaag cgatcaggcg gcgtcgtgac ctgatacgtc tgcggcaacg
     5161 agcaccgccg agggttccac gcgctattct gaaaaaaacg tctacaaaaa aacgccgccc
     5221 ccgatcggtc gggacggcgc aaaaaaatgg tgggcgatac tggatttgaa ccagtgacct
     5281 cttgcatgtc aagcaagcgc tctaaccaac tgagctaacc gcccctcgta ttttatggta
     5341 cgccgtgccg cgaactcggt caaggtgtgg catctgaggg ccactgaagc gtaactttct
     5401 aaccgcgggt gcggaaagcc caagcccacc tcgtgaaact cctggagcgt ttggtcaatg
     5461 ttattccggt atgcccgatg agggattcgc ggcgcacgtt acgttcggga attgtcctac
     5521 cgtggactat cgttctcggg tttcttgcag tggcaatgtg cctcgcgacc ggcatccgcg
     5581 cgaccgacaa cgggagggag ggcactcgac cggacgccac gaattcggta tctgcccagc
     5641 ggctgagccg ggtgcctctg cagtccgatt cgcggtctga cagcttccgt gtgaggttcc
     5701 gaacagagtt cgcgaaatct ctgaaaaact cgggatttgc cggcgatgtc aaaccccgga
     5761 atcgtggcgc taccggtgac gtactggtct gggacgttcg cgtgcctgtt ggttactcag
     5821 ttctgcgtgc aaccgccgaa gtgatttctg ttgtccattc ctttggcgga aacgtgtacc
     5881 gggccgagca tgagtacgcc gatccgttga aagtgaagct cgcggttggt gccgcgcatg
     5941 cggtgacgga ttctattctt cttgccaccg accgttcaat tgcctcgcgc gggttgctgg
     6001 cgatagtgat agatgacatg gggtaccgga gcatggcgga atctggcaga ttcgctgatc
     6061 tccctttccc tgtcaatctt gcgtttcttc ccgccgggga gaataccctt gagctggcga
     6121 aacgcgcccg cgcgactcga aagacaatcc tgcttcatct tccgatggag ccggaaaatg
     6181 gcaacgagcc gctggagccg aacaccataa tgaccgccat gagtgatgag gaaattcggg
     6241 agttgacacg ccggcacctg gaatcgattc ccgggttagc gggtgtgaac aaccacatgg
     6301 gttctcgcgc gacgagagac gaacgggtga tgcgtgcggt gcttgccgag gtcagggatc
     6361 ggaacctctt ctttctcgat tcctacacga gtccgggtag cactgcggcg gcaacggcgc
     6421 ggtcgctcgg aatacccacc ctcgaacggg acgttttcct tgacaattcg tccgacattc
     6481 aaaccattca aagggaactg tgggcggcgg cggataaggc ttcgaggtcc ggccgcgccg
     6541 tggcgatagg tcacgatcgc cccgaaacgt acgaggcgtt gcgtgtgacg attccggcaa
     6601 ttcagaggtc aggggttcgc gtgtgttttc tgtgggactt gctccgttga agcgtgtcct
     6661 tgcggcgatt gcagttgcct gttcaaccgg tgtgctcggc tgtgcggcgt tcgcgcctcg
     6721 cccggctcac gtaaccgcgg aacacctcga cccgttacag gcctttacgg tgctcaaaga
     6781 gtcaccacgc acgtacaagg tccgagtcgg gagtcttcgc aggtatcgcg tgtccgcacc
     6841 atttgttggg caagagcccg ttcaggtcta cgacctgccg gcggaacttg gggaactgtg
     6901 ggacgaggcg acgtcagagg cgctgtatgg aaatccgcac cgtagcgcgc agatgcttga
     6961 agcgattgta aatgcggaac ctgcgtgcgc ggaatgcagg catgagtatg cccgtgttct
     7021 gataaacctg gcgaacaaag aaaaactcac aaaggaacgc gcacacgagc tgcggtatcg
     7081 cgcactggac gaaatgacga tcgccggtgt tctcgatcgc gagtgcccct caggggcggg
     7141 cgcgttttac cctccgactt ccgccggacg aactgatatc ccccttctgc ctcgcacaag
     7201 acaggcaggg gagttgttcg ccgcgggaaa tgatgcgttc aacagcggcg agttcacgca
     7261 agcgcgttcg ctttacgggg tcgttgtgca gcgcgaaccg acattcggac gtggctacgt
     7321 cggattgggg gatgttttcg tggaactggg cgcctatcag gaggccgccg agtatttcac
     7381 ccgggcactg agcgtggaca gcacggattt cttagcgtgg cgctcacttg ccgagtgcgc
     7441 atttgcgtca ggaaatgcgg aacttgcgaa agaggctgcc gtaagggc
//
LOCUS       DAOSOO010000162         7423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10000_length_7423_cov_5.839441, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000162 DAOSOO010000000
VERSION     DAOSOO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10000_length_7423_cov_5.839441"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="PLT86_10785"
     CDS             <1..2601
                     /locus_tag="PLT86_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT37136.1"
                     /translation="LVNCSFERADGDAPLGWAVLAGSATVVERPGDGHALLLNGRVRS
                     NRFEMSARTPYEWTGEAKGKVTGVVHIIEEDRYLRCETFSHESADWAPFCWRFLPPPN
                     AERAECELVAEGALVDNLYLDGLGAQDYDILDSQAGYHPASEKRIIIRSRTPVEGAIR
                     WELFDTLRGRNYAEGTLEPTGRDMWARWTWVADFTSLRREGYYLLRVHMPDRIVESAP
                     IRIWNGVYEHLAYTVAKYSYLQRCGVEIPGYHKPCHTNDALWRSVAQDETYGKVLEYR
                     DLRGGWHDAGDYNKWFHYFGYVLETLALMHKRVDLPKQTYGGDIPDVLSEIFWGADFF
                     LKVQNPDGSFVGPIHAWYTHVRPETGERYNSNWAIFWGEKIEEDSGHGEVMHPRSRFY
                     DYTSHNNGGLVLDLATALATAARCARGVDDPRRWTYANAALKSGAHVEKTYPGMAGHP
                     YWVTFWYDLYRATDRPEYRENAFALVDPLLAQQAEDGSFGRPAGLKHTFHPITVLMEL
                     LVDEPAHPAREKILRAVERKLAWMEPYTLGRPWDLALQCASGGERGELNGGTMGRNAW
                     VGNVAYVYALAGRLTGRREWLHKSEAQLAWLLGRNPHGVSQVVDAGRVNVGRYHGWSN
                     HCENDQQGRLTGGIINGILTTDDAYPDSRNWTIMPPRFPVLSVRREDVPYAEHAIHNA
                     RHDTNEYWSLHHAGFQQAVSALAAAYKGLEEPERPRLAYLYRNFWSYDAATACDELFD
                     RAGWNADRIASDSRYGHFDTSAYRAMVVSRSWTGSEFIDAEPFGIQMRHSFLRGVPWI
                     FLPPEDPVCLDWLEEFSSGQVPCGEVGKPEGTWEPCRYGRVRTMHGAKVYLVNDEAAL
                     ERLLVDLRTV"
     gene            2838..3818
                     /locus_tag="PLT86_10790"
     CDS             2838..3818
                     /locus_tag="PLT86_10790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT37137.1"
                     /translation="MQANTCVNPWGPIVLFAGRSIPDTARAIAKELGLELSKAMVTQF
                     PDTESYVLLQENVRGADVFIIQSTCAPVNETYMELLVMIDAVRRASASRITVIMPYMG
                     YARQDRKATGREPISAKLMADLVTTAGADRAVSVDLHNSAIQGFFHVPLDHLTAIPAM
                     AAELKKLSLIDPVLIAPDVGRAKLVEKFQEILHIPMAIAHKRRKGLDVTVTEIIGEVE
                     DKTPVIVDDIIASGSIVRHIDALVERGARPEIYMAITHPVLVGKAVERLGHPALKMLI
                     TTDTIPIPPEKQSPKIRVVSTAPMLADVIRRIHTNESVSEMFAKLFEFPV"
     gene            3903..4850
                     /locus_tag="PLT86_10795"
     CDS             3903..4850
                     /locus_tag="PLT86_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37138.1"
                     /translation="MLAFGPIPSRRLGHSLGINNIPPKVCSFNCVYCQVGTTSEMAIE
                     RRVFYSTEQIVEAVGAKVNDARASGEKIDYLAFVPDGEPSLDINLGAHISALREFGIP
                     VAVISNSSLIWREDVRADLARADWVSLKVDSVREETWRKIDHPHGGLSLPAILDGARE
                     FASGFKGKLVSETMLVAHLNDSEEEMDAVGVFLAQLQPAMAYLMIPTRPPAKDWVEFP
                     DDAALVAAHRALSLRFPRVELLTKYEGDAFASLGDAGDALLGILAVHPMREDAVLSFL
                     ARAGADVNVLDQALQKGLVVKTTYKGQAFYVRRRAKETR"
     gene            complement(4914..6317)
                     /locus_tag="PLT86_10800"
     CDS             complement(4914..6317)
                     /locus_tag="PLT86_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37139.1"
                     /translation="MPERPNVLFLMNDHTNAEAVAPGSPCIKPNLDLLAREGIRFERC
                     HTTNAICSPARASLMTSLYPSTHGMWDCTHTQRPEWVDVPASRFTYFSTQLANAGYTN
                     GYFGKWHVEQSLRLEDFGWHEYDVNIGSMRHAPAEHGKVVVPKEGYRDYLLCGVDSSG
                     EEYTHPAFDRGCDFIRRTAADGKPFCCFVSTSEPHDPYVPPQRFLDLYDISSIPLSPT
                     LREEPSGKPEVVRRMRAEWSSLTDADWRRIRASYWAVITAVDAEIGRILNVLHETGQY
                     DNTIIVFLSDHGDMLGSHGLATKGVGTAYEEVHNVPLIVRGPGIKRGIEDCRTMTSLV
                     DLAPTILDLCGADPLPRAQGRSLRPVLDGTADPADWEDAYAEFFGQRFVYTQRVTWHG
                     DWKYVFSPGGVDELYNLASDPHERHNLAEDPAHREVLIDMCKRMWRKMKEIGDESLFN
                     THYATLRTAPIGPLSVM"
     gene            complement(6385..>7423)
                     /locus_tag="PLT86_10805"
     CDS             complement(6385..>7423)
                     /locus_tag="PLT86_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37140.1"
                     /translation="DVVQGGVLVLGGVLTIAIVFLDVPGGGAEIIQRASAAGKFDMAA
                     SFDWSLSRETVWVFVLMGLVSNLQELATDQTKVQRYQAAKTDKGAIGATLTVIACLPV
                     WAMFMFIGTCLWAYYSDFPNLLPAGIKADYVYPHFILTQMPTFVAGLVISAVLAAAMS
                     SVDSSMNGSSAALLEDFYKRHLAPGRTDTHYLRVARGITAVLGILMMVIAWALSLLQA
                     DTILEMWIFINAVVAGGLGGFFLLGFLFPHANDRGARIGVAAGVVAILWCTLSHLNPQ
                     FGLLPGWIVLNVHPYLIGVVGNVVVLLVGIAASLFFAKPTEQQLAGMTWWTRDRRPEQ
                     EVLATSVSSGL"
BASE COUNT         1458 a         2159 c         2382 g         1424 t
ORIGIN      
        1 ctggtgaact gctcctttga gcgcgccgat ggcgatgcgc cgctggggtg ggcggttctg
       61 gccggttcgg caaccgtcgt tgaacgcccc ggcgacggcc atgcgctgct gctcaacggc
      121 cgcgtgcggt cgaaccgctt cgagatgagc gctcggacac cctacgagtg gacgggcgaa
      181 gcgaagggga aggttaccgg cgtcgttcat atcatcgaag aggaccggta tctccggtgc
      241 gaaaccttct cgcatgagtc ggcggactgg gcgccgttct gctggcgatt cctcccccca
      301 cccaacgcgg aacgcgcgga gtgcgagctc gttgccgagg gagcgctcgt agacaatctc
      361 tacctcgacg gtctcggcgc tcaggattac gacatcctag actcgcaggc cgggtaccat
      421 ccggcgagcg agaaacggat catcattcgt tcccgcacgc ccgtggaagg cgcgattcgc
      481 tgggagctat ttgacacact gcgcggccgg aactacgcgg aaggcacgct cgaaccgacg
      541 ggtcgcgaca tgtgggcccg ctggacgtgg gtcgccgatt tcacctcgct gcgtcgcgag
      601 ggatactacc tgctgcgtgt gcacatgccg gacaggatcg tcgaatccgc gccgatccgc
      661 atctggaacg gcgtatatga gcatctcgcc tacacggtgg cgaagtacag ctatctccag
      721 cgctgcggcg tagagattcc cggctaccac aagccctgcc acaccaacga cgcgctctgg
      781 cggagcgttg cacaggacga gacgtacggg aaggtgcttg agtaccgcga cctgcggggc
      841 ggctggcacg atgcgggcga ctacaacaag tggttccact atttcgggta tgtgcttgaa
      901 acgctggcgc tgatgcacaa acgggtggat ctgccgaaac agacctatgg cggcgacatc
      961 cccgatgtgc tgtcagaaat attctggggt gcggatttct tcctgaaggt gcagaacccc
     1021 gacggctcat tcgtcgggcc gatccacgcg tggtacaccc acgtgcgccc agaaaccggc
     1081 gaacggtaca actcgaactg ggcgatcttc tggggggaaa agatcgagga ggactccggc
     1141 cacggagagg tcatgcaccc gcgcagccgg ttttacgact acaccagcca caacaacggt
     1201 ggcctggtgc tcgatctggc caccgcgctt gccaccgccg cccggtgtgc acggggagta
     1261 gacgatccgc gccggtggac ctacgccaac gcggcgctca agagcggcgc gcacgtggag
     1321 aaaacctacc ccggcatggc agggcatcct tactgggtga cgttctggta cgacctctac
     1381 cgcgccaccg atcggccgga gtaccgtgaa aacgcgttcg cccttgtgga cccgttgcta
     1441 gcccagcagg cggaagacgg gtcattcggg cggcccgcgg ggctcaaaca tacgttccac
     1501 ccgatcacgg tgttgatgga gctccttgtg gacgagccgg cacatccggc gcgggagaag
     1561 atactgcggg cagtcgaacg caaactggcg tggatggagc cgtacaccct cggccggccg
     1621 tgggatcttg ccctgcagtg cgccagtggc ggcgagaggg gtgaactcaa cggcggtaca
     1681 atggggcgca atgcgtgggt tggcaacgtc gcgtatgtgt atgcgctggc gggccgtctc
     1741 accggcaggc gtgaatggct tcacaaatcg gaagcccagc tcgcctggct gctcggtcgc
     1801 aacccgcacg gcgtctcgca ggtggtggac gccggcaggg tgaacgtcgg gcgctaccac
     1861 ggatggtcga accactgcga aaacgatcaa caggggcggc tcaccggcgg gatcatcaac
     1921 ggaatcctga cgaccgacga tgcatatccg gacagcagaa actggacgat catgcctccc
     1981 aggttccccg tgttgagcgt gcgcagggag gatgtgccgt acgccgaaca cgcgatacac
     2041 aacgcccgcc atgacacgaa cgagtactgg tcactgcacc acgcagggtt tcagcaggcg
     2101 gtcagcgccc ttgcggcggc gtacaagggg ctggaagagc cggaacgacc gcgactggcg
     2161 tacctctacc gcaatttctg gtcgtacgat gcggcgaccg catgtgacga gcttttcgat
     2221 cgcgccggct ggaatgcgga tcgcattgcc agtgacagcc gttacgggca cttcgatacg
     2281 tctgcgtacc gggcgatggt ggtgagcaga tcgtggacgg gcagtgagtt catcgacgcc
     2341 gaaccgttcg gcatccaaat gcgacactcg ttccttcgtg gcgtcccctg gatttttctt
     2401 ccgcctgaag accctgtctg tctcgactgg ctggaggagt tttcttcggg gcaggtcccg
     2461 tgcggggaag tcgggaagcc ggaaggaacg tgggagccgt gccggtatgg cagggtaaga
     2521 accatgcacg gcgcaaaggt gtacctggtg aatgacgagg ctgcgctgga gcgccttctg
     2581 gtggacctca ggacggtctg acgcaccggg aggcttcgcc gacatgcgtg cgccgcttcc
     2641 gggggaattg tcggagaggt gtgagtctgc tcgtgtttcg cgcacttgtg tcgtgctcca
     2701 ttgaccgctc cgctctgagt gccgattttc attactcggg gcaatgtctc tccgccccct
     2761 gtttctagtt cgacggaagc cgtgtttcac gtggtgcctc gtgccgtatc ggggtgcgcg
     2821 acgcagaagg aaaagccgtg caagcaaata cgtgtgtgaa cccgtgggga ccgatcgtgt
     2881 tgttcgccgg gcgttccata ccggataccg cccgggccat cgccaaagag ctggggctcg
     2941 aactcagcaa agccatggtg acgcagttcc ccgacacgga gagctatgtc ctgctgcagg
     3001 aaaacgtgcg aggggctgac gtatttatca ttcagtccac gtgtgcgccg gtgaatgaga
     3061 cgtacatgga actgctcgtc atgatcgacg ccgttcgtcg ggcgtcggca tcgcgcatca
     3121 cggttatcat gccttacatg gggtacgcaa gacaggaccg caaggctacg ggccgcgaac
     3181 cgatcagcgc aaagctcatg gcggatctcg tcaccaccgc tggagccgac cgcgcggtgt
     3241 cggtggacct tcacaactcg gcaatccagg ggttctttca tgttccgctg gaccatctca
     3301 cggctattcc tgccatggcg gcggagctga agaagctgag cctgattgac ccggtgctta
     3361 ttgccccgga cgtcggccgc gcgaagctgg tggagaagtt ccaggaaatc ctccacattc
     3421 ccatggcgat tgcgcataag cggcgaaaag gtctcgacgt gacggtgacg gagatcatcg
     3481 gcgaggttga agacaaaacg ccggtcatag tggacgatat catcgccagc gggagtattg
     3541 ttcgccacat tgacgcgctg gtggagcgag gtgcgcgacc ggaaatctac atggcgatca
     3601 cccatcccgt gcttgtgggg aaagccgtgg aacggcttgg ccacccggcg ttgaagatgc
     3661 ttatcacgac cgacacgata ccgattcctc cggagaaaca atcgccgaag attcgcgttg
     3721 tcagcacggc accgatgctg gctgacgtta tccggaggat tcatacgaac gagtcggtgt
     3781 cggagatgtt cgcaaagctg tttgaattcc ccgtctgagc aatcttcgca aggctgcagt
     3841 atccttcatc gggaggacgc caaggcattt cgcagtcctc ccgagccatc cggagtgcca
     3901 ccatgctcgc atttggtcca ataccttcga ggcgcctcgg ccacagcctc gggatcaata
     3961 acattccgcc caaggtctgt tcgttcaact gcgtgtactg ccaggtcggc accacctccg
     4021 agatggcgat cgagcggcgg gttttttact ccacggagca gatagttgag gcggtgggcg
     4081 caaaagtcaa cgatgcccgc gcgtcgggag agaagatcga ttacctcgcg ttcgtgcccg
     4141 atggagagcc gtcgctggat atcaatctgg gtgctcacat ttctgcgttg cgggagtttg
     4201 gtatcccggt tgcggtgata agcaattcgt ccctgatctg gcgggaggat gtgcgagcgg
     4261 accttgccag ggccgactgg gtgtccctca aagtggattc ggtcagggag gaaacctgga
     4321 ggaaaatcga ccatccccac ggcgggctgt cacttcccgc cattctggat ggggcgcgtg
     4381 agtttgcttc cgggttcaaa gggaagcttg tttcggaaac gatgctggtg gcccacctga
     4441 acgacagcga agaggaaatg gacgcggtag gagtatttct cgcgcagttg cagccggcaa
     4501 tggcgtatct gatgattccg acacgcccgc cggcaaagga ctgggtcgag ttccctgatg
     4561 atgccgcgct tgtggccgcg caccgcgcgc tgagccttcg gtttccgcga gtggagctgc
     4621 tgacgaagta cgaaggggac gcattcgcat cactgggaga tgcgggcgac gcgctgctgg
     4681 gaattcttgc cgtgcatccc atgcgagagg atgcggtttt gtcgttcctt gcacgcgccg
     4741 gtgcggatgt caatgtgctt gatcaggccc tgcagaaggg gcttgtggtc aaaaccacgt
     4801 acaaagggca ggcgttctat gtgagacgcc gcgcgaaaga aacgcggtga gatttgaaga
     4861 ccgcaggaac agaaaaggag ggcgcgcatg tggcgccctc ctttttttgg tgatcacatg
     4921 acagacagcg gaccgatcgg ggcggttcgc aacgtggcgt aatgcgtgtt gaaaagggac
     4981 tcgtcgccga tttccttcat tttgcgccac atgcgcttgc acatgtcgat aagcacctcc
     5041 cgatgagcag ggtcctcggc gaggttgtgg cgctcgtgcg ggtcggatgc aaggttgtac
     5101 agctcgtcca cgccgccggg gctgaagaca tacttccagt ccccgtgcca ggtgacgcgc
     5161 tgggtgtaga cgaatcgttg cccgaagaat tctgcgtacg cgtcctccca gtctgccggg
     5221 tcggcggtgc cgtcgagcac cgggcgcagt gatctaccct gagcgcgggg aagtggatcc
     5281 gcaccgcaca gatcaagaat tgtcggcgca agatcgacca gactggtcat tgtccggcag
     5341 tcctcaatcc ctcgtttgat tccgggtcct cgcacgatca gcgggacatt gtgcacttcc
     5401 tcgtatgcag ttccaacgcc cttcgtggcc agcccgtggg aacccagcat atcgccgtgg
     5461 tcgctcagaa atacgatgat ggtgttgtcg tactggcctg tttcgtggag cacattcagg
     5521 atcctgccga tctccgcatc taccgcggtg atgactgccc agtaggaggc acggattctc
     5581 cgccagtcgg cgtcggtgag gctgctccac tcggcccgca tgcgtcgaac cacttccggt
     5641 ttgcctgagg gctcctctcg gagagtgggg ctcaacggga tactgctgat gtcgtagaga
     5701 tcgaggaagc gctggggcgg tacgtaaggg tcgtgcggtt ctgaggtgga aacaaagcag
     5761 cagaacggtt tcccgtcggc tgccgtgcgt cgtatgaaat cgcagcctct gtcaaacgcg
     5821 gggtgcgtgt actcctctcc cgagctgtcc acgccgcaca gcaggtaatc gcggtagcct
     5881 tccttcggca caacaacctt cccgtgttcc gccggcgcgt gccgcatgct gccgatattt
     5941 acgtcatact cgtgccagcc gaagtcttca agcctgagcg attgctcgac gtgccatttc
     6001 ccgaagtagc cgttcgtgta ccctgcgttg gccagttgag tggagaaata ggtgaagcgg
     6061 cttgccggaa cgtccaccca ttcgggccgt tgagtgtgcg tgcaatccca catgccgtgc
     6121 gtcgacgggt acagcgatgt catgagcgat gcgcgcgcgg gcgagcagat ggcgttcgtc
     6181 gtgtgacatc gttcaaagcg gataccctcg cgcgccagca gatcaaggtt cggcttgatg
     6241 cacggactgc cgggggcaac tgcctcggcg ttggtgtggt cgttcatgag gaacagaacg
     6301 ttggggcgtt ctggcatggt cagtctcccg gtgcagaagg tgaaaatcgc cgattcctgc
     6361 aaagtgtaag gcggatcagt gctgtcatag accggatgag acggatgtag cgagcacctc
     6421 ttgttcaggg cgcctgtcgc gcgtccacca cgtcatgccg gcgagctgct gttccgttgg
     6481 tttcgcgaag aacaggcttg cggctattcc gacaagcagc accaccacgt tgccgaccac
     6541 cccgatgagg taggggtgta cgttcagaac aatccagccg gggagcaacc cgaactgcgg
     6601 gttcaggtga ctcagggtgc accagaggat cgcaactacc cccgcagcca cgccgattct
     6661 ggctcctctg tcgtttgcat gcgggaagag gaagcccagc aggaaaaagc cgccaaggcc
     6721 gcccgccacc actgcgttga tgaatatcca catttcgagg atggtatcgg cctggagcag
     6781 ggaaagcgcc cacgcgatca ccatcatcag gatgcccagc accgccgtga tgccacgggc
     6841 gacgcgaaga taatgcgtgt cggtgcgccc cggggcgaga tgccgcttgt agaagtcctc
     6901 aagcagggcc gccgaagatc cgttcatgct ggagtcgacg ctcgacatgg cggcggcaag
     6961 aacggccgag ataacaagcc ccgcgacgaa ggtcggcatc tgcgtcagga tgaagtgggg
     7021 atagacgtaa tcggccttga ttccggcggg aaggaggttc gggaagtctg agtagtatgc
     7081 ccacaggcag gtcccgatga acatgaacat tgcccacacc gggagacagg cgatcaccgt
     7141 caacgtggcg ccgatagcgc ctttatcggt ttttgccgcc tggtaccgct gcactttcgt
     7201 ctgatcggtc gccagttcct gcagattgct caccaatccc atcaaaacga atacccagac
     7261 tgtctcccgg ctaaggctcc agtcgaacga agccgccatg tcaaacttcc cggccgctga
     7321 tgctcgctgg atgatctccg cgccaccgcc cggcacatcg aggaacacaa tggctatggt
     7381 gagcacgccg cccagaacaa gaacgccacc ctgaaccacg tcg
//
LOCUS       DAOSOO010000163         7285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10247_length_7285_cov_5.789765, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000163 DAOSOO010000000
VERSION     DAOSOO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10247_length_7285_cov_5.789765"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1759)
                     /locus_tag="PLT86_10810"
     CDS             complement(155..1759)
                     /locus_tag="PLT86_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37141.1"
                     /translation="MQIYAPFTVDGVPPLGTYGTGSLAELAELKECGMNLILGGHAQV
                     DLSTPEGKFCHENGIRVMYHLTQHLYGNPQLAESVDATQTTIPLARVPATRRDARLIE
                     IEGERIRYREMTDSALVGCERGVDGTTPAPHRKGIILFWPEPLEAEVARVKDSPNLWG
                     YYVLDDSPGDAISALRGMYRGIKRMDTANHPVCAGYGGAAAICNFGPGVCDMMLIYFY
                     PCMEGGYVRSFVTQETQWLLSRARERVPGIPFIGVYQGFWGIAGYPDIPVTPHQLREQ
                     MEDFVRDGASGLVAFTARSKAHGDTFDGWNARADLREEMRRINEEIRTTGGLSVPPEP
                     PELASLRIQPTGFWEKPLPIPGLVTAWHVIGPFKDVEKKILDAVFPPEREINLDATYD
                     SAKGPLRWQVFSHEDVYQLNGVLGGDPIYTEEWQQELMNVTAYLTCTVTSPREQEVVL
                     RVGSDDDAILWINGREVWRHDGERGILRDQDTARATLAKGRNVFLMKLYNRRDMWGAS
                     LRITDEGGRAIDGVTISPTADDIAPA"
     gene            complement(1795..4023)
                     /locus_tag="PLT86_10815"
     CDS             complement(1795..4023)
                     /locus_tag="PLT86_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT37142.1"
                     /translation="MQPAAPNSPNRPYSHASFRGGTIPGVCWRSGSAVFDEMFRDGRL
                     VCRYWNANGQIWPEMHFENTAWAEDQPADTFHLAINRHDLAGGYSWLGAEATPDPSAY
                     RQGVSGSDELPVTHGVIRLAHAEAGVEVAVHTRLDGGPFIIRWLEITNLSSVSVAISS
                     VSPFAGALWAHRYEEHLPAGLSSPFEVAYNHAFEWGHEGDFWFQPLMPGVFAVDGGKK
                     GRSGWGRPAFWARNLCNGQTFVCELAWGGNYEFALDCRFHPRTAQDPTRLDTPRAELY
                     FRMGLSGYDEALRVVEPGETVTTPAVHAALFHADTDDIVQATHQHVRHVVMPAQIPGR
                     EMEIEANHRGYLCDRENVPDILKDVDVAAAAGAEMYLIDAGWYGNEPNRWGNNVGDWH
                     DGAWMKEGGGLKAVADYVHEKGLKFGLWVEIEAAGANSTLKKEHPDWLMTRDGEPVAG
                     GRALDLTKPEVAEFVENEIARLIRTYDLDMFRIDHNHCIQPAGNRRVHGFTEDLIWRY
                     YDVLYGIFDRLRARFPNVVFQNCAGGGGRLDWGTLARFHNTELSDWMRMPRGLKILNG
                     VTMSLPPEILLRTFGTEVGEHALEGDVHTQLRLCFCRPIFRGIAPSMDALTPCLRDRI
                     THYLEVFRKHIRPIMRDGLVFHHTPFLSHQEITPWCVLEYASPDRDRAVAAVFRTSAE
                     GPGEYLFRPRGLDLSTTYRVVLDNRNQSFRATGRDLVTDGVRLQIDRTLASELIVCEA
                     VR"
     gene            complement(4182..4985)
                     /locus_tag="PLT86_10820"
     CDS             complement(4182..4985)
                     /locus_tag="PLT86_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT37143.1"
                     /translation="MDTLLDALQEGRLFELPENDKSHALQFLAHIIEAFPETPAGTDV
                     VETVMKREQVATTAIGKGWACPHARIPGDEDLMCVVGWSPAGIDYGSPDGAPVSLVVM
                     YLVPDNQRNHYLREISSLAKAIESYSGGEKLREAADLDDVRGYLLDLIETGEKTVVPD
                     TRARMIRLQTKPAREAAAVQDLSNLIVEPVTIVAGPGMNHVVLAQNPVLFEQLQSVSG
                     LAERLDAEGLFQNGGWRVLKRSSTLYQGGRVAHDCLAIRMAPQTPARTA"
     gene            complement(4988..>7285)
                     /gene="mgtA"
                     /locus_tag="PLT86_10825"
     CDS             complement(4988..>7285)
                     /gene="mgtA"
                     /locus_tag="PLT86_10825"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOT37144.1"
                     /translation="GLSYVLDQRSSRAVESLGKRVQSRTFVLRDGLEKEIKSSEIVAG
                     DVVLLHAGTIVPADLRLIVAKDFFVSESSLTGESMAVEKTVASAGSSVQSALEMPNAC
                     FLGTSVTSGTARGLVVNTGSRTLFGAISARLSERSEETSFDRGVRSFTWLMIRFMVVM
                     VCVVFFIVGLTKGNWVEALLFGLSVAVGLTPEMLPMIVTVNLAKGALTMAKKKVIVKR
                     LPAIQNFGAIDILCTDKTGTLTQDRVVLERHVDVLGKTTEDVLNYAYLNSYFQTGLRN
                     LIDRAVIDSADLDADLKCRLVDELPFDFQRRRMSVVVDYEGDHVLICKGAVEEIYACC
                     GSYQIDDEVYPLIDMIRTDLFEEVERLNRDGFRVLGIAYREFPRTKTVFTVADESELI
                     LLGYIAFFDPPKPSATEAIELLKKAGVKVKVLTGDNGLVTEKVCRNVELEVDRIVTGT
                     ELSELSPADFLETVQNNNVFVKLSPAQKEQIVKELRKNGNVVGFMGDGINDAPALKAA
                     DVGISVDSAVDVAKESADIVLLEKSLLVLEEGIMEGRRVFANIVKYIRMGASSNFGNM
                     FSVLGASYLLPFLPMQPVQILTNNLLYDFSQTGIPADNVDEELLAKPLKWNISNVKRF
                     MMFVGPISSIFDYATFALMWFFFRSSEYLNPSLGGAEKSALASLFQTGWFVESLLTQT
                     LIVHIIRTRRIPFFGSRASLHMTLTTLAVVAVGAWLPYSPFAPGLGMTPLPAVYWAWI
                     AAFLVSYGILTHLVKTWFFNRYGGD"
BASE COUNT         1378 a         2297 c         2172 g         1438 t
ORIGIN      
        1 gcttcccgtg tcgtccccgc ttcggtaacg cggcagaggg cgtcaagctg gatgcgggcg
       61 gtgttctcgc cgttcgggac agtgaacgtc atatacgagt tggtgaagtc cgtgactggc
      121 atgggcgaat cctccttcag gagaactggg tgggtcacgc cggtgcaatg tcgtctgcag
      181 tggggctgat cgtcacgccg tcaatcgccc ggccgccttc atccgtgatt cggagcgacg
      241 cgccccacat atcgcggcga ttgtacagct tcatcaggaa cacgttccgt cccttcgcga
      301 gtgttgcccg cgcggtatcc tgatcgcgca ggatgccccg ctccccgtcg tgtcgccaga
      361 cctcgcggcc attgatccac aggatcgcgt cgtcgtcgct gccgacgcgg agaacgacct
      421 cctgttcgcg cgggctcgtc accgtgcatg tcaggtaggc ggttacgttc atcagctcct
      481 gttgccattc ctcggtgtag atcggatcgc cgccaagtac accattgagc tggtagacat
      541 cctcgtgcga gaagacctgc caccgaagcg gtcctttcgc gctgtcgtac gtcgcgtcaa
      601 ggttgatttc gcgctccggg gggaacaccg cgtccagaat cttcttttct acgtccttga
      661 agggcccgat cacgtgccac gcggtgacaa ggccggggat cgggaggggt ttttcccaga
      721 acccggtggg ctggatacga aggctggcaa gttccggcgg ctccggcgga accgagagtc
      781 ctccggtggt gcggatctcc tcgttgatcc tgcgcatctc ctcacgaagg tccgcccgcg
      841 cgttccaccc gtcgaacgtg tcgccgtgcg ctttcgaacg ggccgtgaac gccacgaggc
      901 ccgaggcgcc atcccggacg aaatcctcca tctgctcgcg gagctgatgc ggggtaacgg
      961 ggatatcggg atagccggcg atgccccaga acccctgata cacgccgatg aacgggatac
     1021 ccggaacccg ctcccgcgcg cggctgagaa gccattgcgt ctcctgagtg acgaacgacc
     1081 tgacgtaccc gccttccatg cacgggtaga agtaaatcag catcatgtcg cacacgccgg
     1141 gaccgaagtt gcagatggcc gctgcgccgc catatcccgc gcagaccgga tggttcgcgg
     1201 tgtccatgcg tttgatgccc ctgtacatcc cgcgcagcgc cgagatcgca tctccggggc
     1261 tgtcatccag cacatagtag ccccagaggt tcggggagtc cttcacccgc gccacctccg
     1321 cctccagcgg ctccggccag aagaggatga tccccttccg atggggagcc ggcgtggtgc
     1381 cgtcaacgcc ccgttcgcag cccacaagcg ccgagtccgt catctcccgg tagcggattc
     1441 gttctccttc gatctcgatg aggcgcgcat cacgccgcgt tgccggtaca cgcgccagcg
     1501 ggatggtggt ctgcgtcgcg tccaccgact cggcaagctg tgggttgccg tacagatgct
     1561 gtgtcaggtg gtacatcacc cgaatgccgt tctcgtgaca gaacttccct tccggcgtgc
     1621 tgagatcaac ctgcgcatgc ccccccagaa tcaggttcat gccgcattct ttcaactccg
     1681 ccagttccgc gagggaaccg gtgccgtagg tgccgagggg tggaacgcca tcaaccgtga
     1741 acggcgcgta aatctgcatg tctggattcc cttccgaggt ggtggtacgc ctctttaacg
     1801 aacagcctca catacgatca attctgacgc gagcgtgcgg tcgatttgca gccggacgcc
     1861 gtcggtgacc aggtctctgc ccgtcgcccg gaacgactgg ttgcggttat ccagcacgac
     1921 gcggtacgtg gttgaaagat cgagcccgcg cgggcggaag aggtactctc ccggcccttc
     1981 ggcggacgtc ctgaacactg ccgcgacggc gcggtcgcgg tccggcgacg catactccag
     2041 cacacaccac ggggttattt cctggtgtga aaggaacggt gtatggtgaa ataccagtcc
     2101 atcccgcatg attggccgga tgtgcttccg gaatacctcg aggtagtggg tgatgcggtc
     2161 gcggaggcag ggagtcaggg catccatcga tggcgcgatg ccgcggaaga tggggcggca
     2221 gaagcagagc cggagctggg tatgcacgtc gccctccagc gcatgctcac ccacttccgt
     2281 gccgaacgta cgcaggagta tctccggagg aagcgacatt gttacgccat tcaggatctt
     2341 caggccacgc ggcatgcgca tccagtcgga gagctctgtg ttgtggaacc gcgccagcgt
     2401 cccccagtcg agccgtccgc cgcctccggc gcagttctgg aatacgacgt tcgggaaccg
     2461 tgcgcggagg cggtcgaaga ttccgtacag cacatcgtag taccgccaga tcaggtcctc
     2521 ggtgaacccg tgcacgcggc ggttccctgc aggctggatg caatggttgt ggtctatgcg
     2581 gaacatgtcg aggtcgtacg tgcggatcag ccgggcgatc tcgttttcga caaactcagc
     2641 aacttccggc ttcgtcaggt ccagcgcccg ccctcccgca accggttcgc catcccgcgt
     2701 catcagccag tccggatgct ccttcttgag cgtgctgttc gctcccgccg cttcaatttc
     2761 cacccacaac ccgaatttca aacccttttc gtggacataa tccgctaccg ccttcaggcc
     2821 gccgccttct ttcatccacg cgccgtcatg ccagtccccg acgttgtttc cccaccggtt
     2881 cggttcgttc ccgtaccagc ccgcgtctat caggtacatc tcagccccgg ccgctgccgc
     2941 cacatccacg tccttcagga tatccggaac gttctcccgg tcgcacagat agccgcggtg
     3001 gttcgcctcg atttccatct cccgcccggg aatctgggcc ggcatgacaa catggcgcac
     3061 gtgctgatgt gtggcctgca cgatatcgtc ggtatccgcg tggaacagcg ccgcgtgcac
     3121 ggccggcgtc gtcacggttt ctcccggttc aacgacgcgg agtgcttcgt catacccgga
     3181 tagccccatc cggaagtaca gctccgcccg tggcgtgtcg agccgggtgg gatcctgcgc
     3241 agtgcgcggg tgaaaccggc aatccagcgc gaactcgtag ttgccgcccc acgcaagttc
     3301 gcacacgaac gtctgcccgt tgcacaggtt ccgcgcccag aaggccggtc tgccccagcc
     3361 gctgcggcct ttcttgccgc catcaacggc aaacacgccc ggcatgagcg gctggaacca
     3421 gaaatcacct tcgtggcccc actcgaacgc gtggttgtac gccacctcga acgggctgga
     3481 aaggccagcg ggcaggtgct cttcataccg atgcgcccag agcgctccgg cgaagggaga
     3541 taccgaagaa atagcaacag aaacggaact gaggttggtg atttccagcc agcggatgat
     3601 gaacgggccg ccgtcgagac gggtgtgcac ggcaacttct actcccgcct cggcatgggc
     3661 gagccgaatg acgccgtgcg tcaccgggag ttcgtcactg ccgctcaccc cctgccggta
     3721 cgccgatggg tcgggtgtgg cctccgcgcc cagccatgag tacccgccgg cgaggtcgtg
     3781 ccggttgatg gcgaggtgga acgtgtcagc gggttgatct tccgcccatg cggtgttttc
     3841 gaagtgcatc tcaggccata tctgcccgtt cgcgttccag taccggcaca ccaaccggcc
     3901 gtcgcggaac atctcgtcga acaccgcgct gccggagcgc cagcacacac cgggaatggt
     3961 tccgccgcga aaggaagcat gggaataggg ccgattggga gagttcgggg ccgcgggttg
     4021 catggggttg cctcgggaag cgggacgcgc gtggttatct caggcactgt gcaaagtgta
     4081 gcgcggcgcg cttcggggac aaacgtggtg ggggatgtat ggggcctttc cagccggcaa
     4141 ggtgctgcgg cggttgcagg ggctcgaaag ggaacgcggc tctaggccgt gcgcgccggg
     4201 gtctggggag ccatccttat tgccaggcag tcatgcgcga cgcgtccccc ctgataaagg
     4261 gtggaactcc gcttcaacac gcgccacccg ccgttttgaa acaggccttc cgcgtccagc
     4321 cgctcggcaa gcccgctcac ggactggagt tgttcgaaaa gaacgggatt ctgtgccaga
     4381 acaacgtggt tcattcccgg gccggcgacg atcgttacgg gttccacgat caggtttgaa
     4441 aggtcctgaa cggcggccgc ttcccgtgcc ggtttggttt gcaggcggat catccgcgca
     4501 cgcgtgtccg ggacgactgt cttctcgcct gtttcgatga gatcgagcag atacccgcgg
     4561 acgtcgtcaa ggtctgcggc ttcgcgcagt ttttctccgc cggaatagct ttcgatggcc
     4621 tttgccagcg aggaaatctc gcgcaggtag tggtttctct ggttatccgg aacgaggtac
     4681 atgacgacga gcgaaacggg cgctccgtcc ggcgagccgt aatcaattcc ggcggggctc
     4741 catcccacca cgcacataag gtcctcgtcc ccgggaattc gcgcatgagg gcaggcccag
     4801 cctttgccta tggctgtggt ggcaacctgc tctcttttca ttacggtttc caccacgtca
     4861 gtcccggcgg gtgtctccgg aaacgcttcg ataatgtgtg ccagaaactg cagcgcgtgc
     4921 gacttgtcgt tttcaggcag ctcgaaaagc cggccttcct gcagcgcgtc caggagtgta
     4981 tccatcgtca atcacctcca tatcggttga agaaccatgt cttcaccaaa tgcgtcagaa
     5041 tcccgtagga cacaagaaac gcggcaatcc acgcccagta caccgcgggc agcggagtca
     5101 ttcccagacc gggggcgaag ggagagtatg gcagccacgc cccgacagcc accacagcaa
     5161 gcgtggtcaa cgtcatgtgg agggaggctc gacttccgaa aaaggggatt cttctcgtcc
     5221 ggatgatatg gacgatcagt gtctgcgtga ggagcgattc gacgaaccat cccgtctgga
     5281 agaggcttgc gagagcactc ttctcagctc ctcccaggga gggattcagg tactcactgg
     5341 acctgaagaa aaaccacatc agcgcaaacg tggcgtaatc gaagatggag ctgatcggcc
     5401 ccacgaacat catgaatcgc ttgacattcg agatattcca tttgagaggt ttggcgagca
     5461 gttcctcgtc cacgttgtcg gcagggatgc cggtctgcga gaagtcgtaa agcagattgt
     5521 tcgtgagaat ctgtaccggc tgcatcggca ggaacggcaa caggtaactc gccccgagca
     5581 cgctgaacat gttgccaaag ttggagctgg cgcccatgcg gatgtatttg acgatgttgg
     5641 cgaaaacccg ccgcccctcc atgatgccct cttccagcac gagaaggctc ttctcgagca
     5701 gcacgatatc cgcagattcc ttggccacgt ccaccgcgga atctaccgaa atgccgacgt
     5761 ctgccgcctt cagggccggc gcatcgttga taccgtctcc catgaaaccg acaacgttcc
     5821 cgtttttccg cagctccttg acgatctgct ctttctgcgc cggagataac ttgacgaaga
     5881 cgttattgtt ctgcacggtc tccaggaaat cagcgggaga gagctccgat aactccgtgc
     5941 cagtgacgat tctgtcaact tccagctcca cgttgcggca gaccttttcg gtgacaaggc
     6001 cgttatcgcc ggtcagcact ttcaccttga ctcccgcctt tttcaacagc tcgatggctt
     6061 ccgtcgctga gggcttcggc gggtcgaaga acgcgatgta cccgagcagg atgagctcgc
     6121 tttcatccgc gaccgtgaat accgtcttcg tgcgggggaa ttcgcgatag gcgatgccga
     6181 gtacccggaa tccatcgcgg ttgagccgtt ccacctcctc gaagagatcc gtacggatca
     6241 tgtcgatcag cgggtaaacc tcgtcgtcga tctgatagct gccgcaacac gcgtagatct
     6301 cctccaccgc gcccttgcag atcagaacgt ggtccccttc gtaatccacc acaacggaca
     6361 tgcgccggcg ctggaaatcg aagggcagtt cgtccaccag ccggcacttc aggtcagcgt
     6421 ccagatccgc ggagtcaatc accgcgcgat ctatcaggtt gcgaagtccg gtctggaagt
     6481 agctgttgag atacgcatag ttcaggacgt cttcggttgt ttttccgaga acatccacat
     6541 ggcgttcaag aaccacccga tcctgcgtca gggttccggt tttgtccgtg cagagaatgt
     6601 caatggcgcc gaaattctga attgccggga gcctctttac gatgaccttc ttcttcgcca
     6661 tcgtgagtgc gcctttggcc agattcacgg tgacaatcat cggcagcatt tccggggtga
     6721 gcccgacggc gacggaaagg ccgaaaagca gcgcctcaac ccagttgcct ttggtcagac
     6781 cgacgatgaa aaacaccacg cacaccatca ccaccatgaa ccggatcatc agccaggtga
     6841 acgagcgcac gccccggtcg aaactcgttt cttcgctccg ctcggagagc cgcgcggaaa
     6901 tcgccccgaa caacgtccgt gagcccgtgt tgaccaccag tccccgtgcc gtgccgctgg
     6961 tgacggaggt gccaagaaaa caggcgttcg gcatctccaa cgccgattga accgacgagc
     7021 ccgcgcttgc aaccgtcttc tcaacggcca tggactcgcc ggtcagactc gactcgctga
     7081 cgaagaagtc tttggcaact atcagtcgga gatcagcggg aacaatggtg ccggcatgca
     7141 ggaggaccac gtcgccagca acgatttccg agctcttgat ttccttctcc aggccgtccc
     7201 tcagcacaaa agtgcgggat tgcacgcgct tcccaagcga ttccactgct cggctggacc
     7261 gctgatccag tacgtaggat aaccc
//
LOCUS       DAOSOO010000164         7277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10267_length_7277_cov_5.902935, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000164 DAOSOO010000000
VERSION     DAOSOO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10267_length_7277_cov_5.902935"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLT86_10830"
     CDS             complement(<1..292)
                     /locus_tag="PLT86_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37145.1"
                     /translation="MWEAAARGRHLEAFAARPALRTGLGCFAALAFVDSRLRGNDDWG
                     LAKAAAASARLPYLWHTKGATQERRPWGGLWRRWWGKTERGERCRSPRVDAVL"
     gene            860..1798
                     /locus_tag="PLT86_10835"
     CDS             860..1798
                     /locus_tag="PLT86_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT37146.1"
                     /translation="MARLARLVSISFPGVHGEMPPVERARRAVEAVSARIDEAAYDKP
                     DIIVLPETFTALGGDGGKDWFASAEPIPGPTTDALAAKAKQYNTHIVCPILEVRDGKR
                     YNAAALIDRTGSIVGVYHKMHPTNPELEGGIVPGTTAPAWDTDIGRVGCAICFDLNFR
                     DVADSLAAHRAELVCFCSMYRGGLSTQIWAFEYGFWFISATPAENSVIRNPLGQLVVQ
                     SFAYSPTIVAEANLDCVVCHIDCNQEKIPALKKKYGPLAEIHSISPEAVFLLTSRHPT
                     VSARDMVEEFKFETRTEYWKRSHANRAVALMKARES"
     gene            2186..3118
                     /locus_tag="PLT86_10840"
     CDS             2186..3118
                     /locus_tag="PLT86_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOT37147.1"
                     /translation="MASSKPLIWAQLVHLGYNMWADREAPEWNIEYTAAQQTMRFDMS
                     LWDDLMKYLTEVGVNTVVIDLGEGLKYESHPELAVDGSWTGAQLRKKLAEMRALGLTP
                     IPKLNFSTCHDTWLGDYSRCVSSKIYYRVCSDIIAEVCELFDKPALFHLGMDEENQPN
                     QQFYAYSVCRQMELWWHDLYFLAEQAEKGGARPWIWSDYVWDNHDVFYAKMPKSILQS
                     NWYYGHKFTTDITAVKAYNELEEHGYDQIPTGSNWSDPQNFEKTVRYCKDVIAPERLK
                     GFFQTVWKPTVERRRWRHFEGASEVGYGKRAFEE"
     gene            complement(3220..4338)
                     /locus_tag="PLT86_10845"
     CDS             complement(3220..4338)
                     /locus_tag="PLT86_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOT37148.1"
                     /translation="MNQRYDIAAYYWPAYHDEPRWRRFMPKGEGEWQTVRAAAPKFAG
                     HQQPRVPAWGYEDESDPGVMERKIHAAASHGVNTFVFDWYWYENQPFLEEALNRGFLG
                     APNNDRMRFYLMWANHDATTLWDLEQSHEYRVIWPGAADRAAFDAATGRIIERYFGHS
                     SYYLIDGKPVLSIYELGTLITGLGGLEATKDALGSFRERVRAAGFPGVHLQAILWANI
                     PKSPAILPGGGPPADRAETQDNTIRYLGFDSLTNYQWCHYVTPRGEYRAWGEAAVAAW
                     ERWADEFSVPFFPHVSIGWDTNPRFKDLRPDIVTGGTPEAFRGFLIRAMRHVDTRGLI
                     PPLITVNAWNEWSESSYLEPDTVWGMQYLEAVRDALAT"
     gene            complement(4335..5231)
                     /locus_tag="PLT86_10850"
     CDS             complement(4335..5231)
                     /locus_tag="PLT86_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37149.1"
                     /translation="MEHACFELAPTVFTGTRVKTGFHCHTVNSDGGLSPADTARTYRE
                     RGFRALCITDHRHVTDTEGLSDTEFICLPSIENGGMPDILAVGVKAPVERTLPLAERA
                     KLLAAQGGFTVAAHPTYCAALPQDYLGCEHLHALEIYNAYCDNAYVNGYATELWDMLL
                     GQGKRVLGLAGDDAHLNPEKGYYSHAGLGWIEVWAREFSQPALLAAMKQGAFFSTQGP
                     VFETIEVEHDAIRVTCSPVKQVRWRTFGHAGFVDHAPEAASLTQSELPDWLKTRVFVR
                     IELLDHRGKRAWSNPFFLKAQR"
     gene            complement(5317..6279)
                     /locus_tag="PLT86_10855"
     CDS             complement(5317..6279)
                     /locus_tag="PLT86_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOT37150.1"
                     /translation="MAHYTPRVVASLAFAVLVSGCASTTVQVKVLHPAEIDLVGIRRI
                     ALGEISGTGGEELAGTLVTGLFNTGRFEILDRQHLETLMREHQLAAGGLVNPETAAQT
                     GSLVGSAAIVVGRVTEHRFTETLEVGNPKKDDEGNEHRKYTRRGTARVAATLQITDIA
                     TGRVIAVRQPTTVKSATLTSTDAIPGHIDPSPLLAAAREECVADFLRAVAPHEVNESV
                     EFEDDDDLPGTKRGIEFAKGGDLERAVTTFQEVVALHSTKARTHYNLGLALLCLGRYD
                     EAIASLDLAYGMTASRKYARTLARAREWKANAERLRQQSATGTP"
     gene            complement(6454..>7277)
                     /locus_tag="PLT86_10860"
     CDS             complement(6454..>7277)
                     /locus_tag="PLT86_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOT37151.1"
                     /translation="SHAIQTNGTLIDDDWARFFHDHRMLVGLSIDGPPDLHDTYRLDS
                     AGRGTHARLMEVAETLFRNGAEVNVLCTVNRATARKPEAVFRYLVAQGFQWLQFIPVV
                     ERLPNGQPAPFSVTPAQYGEFLKRIFDLWWNDGTPQVSVRLFDEIVSAAMGHPPMMCQ
                     LREACGGIYVVVEYNGDVYPCDFFVEERWRMGNLTETPLAELVNGEVLRRFAEIKPRA
                     TSACDSCPYLTFCRRGCPHYRAIGDRFLDRDYLCEGYRRFYAHALPKLAAKLGRR"
BASE COUNT         1437 a         2330 c         2129 g         1381 t
ORIGIN      
        1 gcagaactgc gtccacacgg ggtgagcggc aacgctcacc ccgttctgtt ttgccccacc
       61 atcttctcca cagtccgccc caagggcgtc tctcctgcgt cgcgcccttc gtgtgccaca
      121 ggtagggcag tctcgccgag gccgccgccg ccttcgccaa cccccaatcg tcattcccgc
      181 gcaggcggga atccacaaac gccaacgccg cgaaacatcc cagcccggtt cgcagggcag
      241 ggcgggctgc aaatgcttcg agatgtctgc cccgcgctgc cgcctcccac aaccgtcatt
      301 cccgcgtgct cggcagagca cgaagcgcag gcgggaatcc agaaccacgg gtcgtgcttg
      361 gtgggacagg cattcctgcc tgtccttgcc gcataccgtg ccggtgtgga gacgcccggc
      421 tctctaagtg gcgtctgccg agggcatgga gggccccgct ccgtcggggc ctttttttcc
      481 ccgctgtcct gctcctgccc caaatgcctc acggcccacg caccggcgtg cgttcagcgc
      541 caacggcgtg taataacaca gcccggttcg cagggccggg cgggctgcaa atgcttcgag
      601 atgtctgccc cacgctgccg ccttgctcac ccgtcattcc cgcgtgctcg gcagagcacg
      661 aagcgcaggc gggaatccag agccacgtgt cgtgcttggt gggacaggca ttcctgcctg
      721 tccttgccgc aaacccttgc ctgcggggcc tccccaccca ttgccctctg caccggctcc
      781 accttgcgcc tgagggactt cccgctgcac tcttcttcag ggcgttcagt cagaactctt
      841 ttgcggagga gccaccggta tggcacgact tgcgcgattg gtcagcatca gtttccccgg
      901 agttcacggc gaaatgccgc ctgtcgagcg tgcacggcgc gcggttgagg cggtgtctgc
      961 ccgaatcgat gaagccgcat acgacaaacc tgatatcatc gtcctgccgg aaaccttcac
     1021 cgcccttggc ggggacggcg ggaaggactg gtttgcctcc gcggagccca tcccggggcc
     1081 cacgacggac gccctggccg caaaggcgaa acagtacaac acccacatcg tctgcccgat
     1141 cctcgaggta cgcgacggca aacgctacaa cgcggctgcg ctcatcgaca ggacgggttc
     1201 catcgtcggc gtctaccaca aaatgcaccc cacgaacccc gaactggagg gcgggatcgt
     1261 tcccggaaca accgctccgg catgggatac cgacatcggt cgcgtcgggt gcgccatctg
     1321 cttcgatctc aatttccggg acgttgccga ttcccttgcg gcgcaccggg cggaactcgt
     1381 ctgtttctgc tccatgtacc gtggcggcct aagcacgcag atatgggcat tcgagtacgg
     1441 attctggttt atttctgcaa cgccggcgga aaacagcgtg attcggaacc cgctgggcca
     1501 gcttgtcgtg cagtcgttcg cgtattcacc cacgatcgtc gcagaggcga accttgattg
     1561 cgtcgtgtgc cacattgact gcaaccagga gaaaatcccc gcgttgaaga agaaatacgg
     1621 gccactggcg gaaatccaca gcatctcccc ggaggccgtg tttctcctga cttcccggca
     1681 cccgacggtg tccgctcgag acatggttga ggagttcaaa ttcgagacca ggacggagta
     1741 ctggaagcgt tctcacgcca acagagcggt tgccctgatg aaagcgcggg agtcatgaat
     1801 cgatgaatag agcgttggtg gcgcttgcgt gtgccatcct gtgcggatgc ggcggcaggc
     1861 gccccgcaaa tacaacggcc gagccctctc agccccctcc cgcggcaatc gttcgcacgg
     1921 aaccgccacg ctatcagcac atcccgcgcg gcacctaccg caacgatatc cccgaagtgg
     1981 aaccggttcg ccggcccgtt cgatccgaca cgacccggcg cgccgcgccc cggccaccgg
     2041 agccgacgga atcactgtat acacaaccag ttcgcacaac taccccgcgc gtcgggccat
     2101 cccgccccga cagcgcgtcc gttcgaccgc tgagccctga ataagcccgc tccggtcacc
     2161 atctcacagc gcgaaaggca acaccgtggc atcctcaaaa cctcttatct gggcgcaact
     2221 tgtgcatctc ggttacaaca tgtgggccga ccgcgaagct cccgaatgga acatcgaata
     2281 caccgccgcg cagcagacga tgcgattcga catgtcgttg tgggacgacc tcatgaagta
     2341 cctcacggag gtcggcgtca ataccgtcgt catcgatctc ggggaaggcc tgaaatacga
     2401 gagccatccc gagctggcgg tggatggctc gtggacgggt gcgcagcttc ggaaaaagct
     2461 cgcggaaatg cgggcgctcg ggttgacacc gattccgaaa ttgaacttct ccacgtgcca
     2521 cgacacctgg ctgggcgatt actcccggtg cgtgtcctcg aagatctact acagagtgtg
     2581 cagcgacatc atcgcggaag tgtgcgagct ctttgacaaa cccgccctct ttcacctcgg
     2641 catggacgag gaaaaccagc ccaaccagca gttctacgcc tattccgtct gtcgccagat
     2701 ggaactctgg tggcacgacc tttacttcct cgcggaacag gccgaaaaag gcggtgcgcg
     2761 gccgtggatc tggtcggatt acgtgtggga taatcacgac gtattctacg cgaaaatgcc
     2821 gaaatccatc ctgcagagca actggtacta cggccacaag ttcacgaccg acataactgc
     2881 agtgaaggcg tacaacgaac ttgaggaaca cggatacgat cagatcccca ccggcagcaa
     2941 ctggagcgac ccgcagaatt ttgagaagac cgtccgttac tgcaaggatg tcatcgcccc
     3001 ggaacggctc aaaggcttct tccagactgt ctggaaaccc accgtggagc gtcgtcgctg
     3061 gcggcatttc gaaggcgcga gcgaagtcgg gtacgggaaa agggcgtttg aggagtagga
     3121 gaagcgtaca gggcggggaa accgcggcaa agggcatcgg tcatcgagtg cgggggcgtt
     3181 caccggtgtg aacgcccccg cgtatcgaga tgcccgatgc tacgtcgcca gtgcatcgcg
     3241 cacagcttcg aggtactgca tcccccagac ggtgtccggt tcgaggtagc tgctttcgct
     3301 ccactcgttc cacgcgttca ccgtgatgag cgggggaatc agcccgcgcg tatccacgtg
     3361 gcgcattgcg cgaatgagga aaccacggaa ggcctcgggc gttccgccgg taacgatatc
     3421 cggtctcaag tctttgaagc gcgggttcgt gtcccagccg atggaaacat gagggaaaaa
     3481 cggcaccgag aactcatcag cccacctttc ccacgcagcg acggcggctt ctccccatgc
     3541 ccggtactca ccccgcgggg tgacgtagtg gcaccactgg tagttcgtca ggctgtcgaa
     3601 accaaggtaa cggatggtat tgtcctgcgt ctcggcgcgg tcggcaggcg ggccgccgcc
     3661 cggcagaatc gcgggtgatt tcgggatgtt ggcccacaga attgcctgca gatgcacgcc
     3721 gggaaaaccg gccgcacgaa cgcgctcccg gaatgacccc agtgcgtcct tcgttgcctc
     3781 aagaccaccc agaccggtga tgagcgtgcc cagttcgtag atggagagaa cgggcttccc
     3841 gtcgatcagg tagtacgagg aatgcccgaa atagcgctcg attatcctgc ctgtggcggc
     3901 atcgaacgcg gccctgtcgg ctgcccccgg ccagatgacc cggtattcgt gcgattgctc
     3961 aagatcccac agcgtggtcg catcgtggtt ggcccacatg aggtagaagc gcatccggtc
     4021 attgttcggc gcaccgagga atcctctgtt cagagcctct tcgaggaacg gctggttttc
     4081 gtaccaatac cagtcgaaaa caaacgtatt gacgccatgt gaagccgccg cgtggatctt
     4141 ccgctccatc acccctggat cagattcgtc ttcgtatccc cacgcgggca cccgcggttg
     4201 ctgatgcccg gcgaacttcg gcgccgcggc gcggacggtt tgccattcgc cctccccctt
     4261 tggcatgaag cgccgccagc ggggctcgtc gtgataagcc ggccagtagt aggccgcaat
     4321 gtcgtaacgc tggttcatcg ttgtgccttg agaaagaaag ggttcgacca ggcgcgtttc
     4381 ccacggtgat cgagcagctc gatgcgcacg aacacccgtg ttttgagcca gtcgggaagc
     4441 tccgactgcg tcagggaggc agcctcggga gcatgatcca caaacccggc gtggccgaac
     4501 gtccgccagc gcacctgttt caccgggctg catgtcacgc ggatggcatc gtgttccact
     4561 tcgatcgttt cgaacaccgg tccctgtgtg gagaaaaacg cgccctgttt catcgcggca
     4621 agcagtgcag gttgggagaa ttcacgcgcc catacctcaa tccaaccgag cccggcgtgt
     4681 gaatagtacc ccttctcggg attcagatgc gcatcgtctc ccgccagccc cagtacccgc
     4741 ttgccctgcc cgagcagcat gtcccagagt tctgtcgcgt agccgttgac gtaggcgtta
     4801 tcgcaatacg cgttgtaaat ctccagcgcg tgcaggtgct cgcagccgag gtagtcctgc
     4861 ggcagcgctg cgcaataggt gggatgggcc gctacggtga acccgccctg cgcggccaga
     4921 agcttcgccc gttccgccaa cggcagtgtt ctctcgaccg gcgctttcac cccaacggcg
     4981 aggatatccg gcatgccgcc gttttcgatg gacgggagac agatgaactc cgtatctgag
     5041 agaccttccg tgtccgtcac gtgacggtga tcggtaatgc agagcgccct gaagccacgc
     5101 tcgcggtacg ttcgcgcagt atcagcaggt gaaaggccgc catcggaatt caccgtgtgg
     5161 cagtgaaacc cggttttgac ccgcgtgccg gtgaaaacgg tcggggcaag ttcaaaacag
     5221 gcgtgttcca caggacctcc agtactcacg ttggaagcaa caaccggttc ttaatgcctt
     5281 ggcggttcgt cgggtctgca gtttttcccg cagtcatcac ggagttcccg ttgcgctctg
     5341 ctgccgaagc cgttccgcat tggccttcca ttcccgggcc cgcgcgagcg ttctggcata
     5401 tttgcgggac gcagtcatgc cgtacgcaag atcgagggag gcaatagcct cgtcatagcg
     5461 gccaaggcaa aggagggcga ggccaaggtt gtaatgcgtc cgtgctttcg tggaatgcaa
     5521 tgcaaccact tcctggaacg tcgtgacggc tcgttccaga tcaccgccct tcgcgaactc
     5581 gatgccacgt ttcgttccgg ggaggtcgtc atcgtcttca aactcaacac tctcgttcac
     5641 ttcgtgcggt gcgacggcac gaaggaaatc agccacgcat tcctcgcgcg cggctgcaag
     5701 gagtggactc gggtcaatat gcccgggaat cgcatcggta gaggtcaatg tggccgattt
     5761 caccgtggtc ggctgtcgaa cggcaatcac ccgcccggtc gcgatgtccg taatctggag
     5821 tgtggcggca actctcgcgg tcccgcgcct ggtgtacttc cggtgttcgt tcccttcgtc
     5881 gtcttttttc gggttgccaa cctcaagcgt ttccgtgaac cggtgttccg ttacccgtcc
     5941 cacaacgatc gccgcacttc ccaccaggct accggtttgg gcggctgttt ccggattcac
     6001 cagacctcct gcggcaagct ggtgctcacg catgagggtt tccagatgct gacgatccag
     6061 aatttcaaat cgcccggtat tgaacagacc cgtcaccagt gtccctgcca gttcctcgcc
     6121 accggtgccc gaaatctctc ccagggcgat gcgccgaatg cccaccagat cgatttccgc
     6181 aggatgaagc accttcacct gcacagtcgt tgatgcgcat ccgctcacaa gaacggcgaa
     6241 cgcaagcgaa gccacgacgc gtggagtgta atgtgccatg gggtcccccg ttcttgccga
     6301 cggcaaccgc cgccgcaggt gcttggtccg cagctggtga gccgtcgccg gaaacgcgca
     6361 aggagttcga atcacccgga gaatcactca catggaaagg tagtgaggtt cgtgcgtggc
     6421 tggaaggacg gcggcggcag acgagcgctg atcttacctc cgtcccagct tcgccgccag
     6481 tttcggcagg gcatgggcgt agaaccgcct gtacccctcg cacaggtaat cgcggtcaag
     6541 gaatcggtcg ccaattgcgc ggtaatgggg acatccgcgc cggcagaacg tgaggtaagg
     6601 acaggaatcg cacgccgacg tggcccgggg tttgatctcg gcgaatctgc gcaacacctc
     6661 gccgttcacg agttcggcaa gaggagtttc cgtaagattg cccatcctcc agcgctcttc
     6721 cacgaaaaaa tcgcagggat acacatcgcc gttgtattcg accacgacat agattccacc
     6781 gcacgcctca cgcaactggc acatcatcgg agggtgtccc attgccgccg atacgatctc
     6841 gtcgaaaagc cgtaccgata cctggggcgt gccgtcgttc caccagaggt cgaatatgcg
     6901 cttcaagaac tcgccatact gcgcgggagt caccgaaaac ggtgccggtt gcccattcgg
     6961 caaccgctca acaaccggga tgaactgcag ccactgaaat ccctgcgcaa cgaggtaccg
     7021 aaacaccgcc tccggcttcc gcgcggttgc acgattcacc gtgcacagca cattcacctc
     7081 ggcgccgttc cggaacagcg tctcggcaac ttccatcaac cgggcatgtg ttccccgtcc
     7141 ggcagaatcc agacgatatg tatcgtgcag gtctggcgga ccgtcaatgc tcaatcccac
     7201 aagcattcga tgatcgtgga aaaatcgcgc ccagtcgtcg tcaatcaacg ttccattcgt
     7261 ctgaatggcg tgggaga
//
LOCUS       DAOSOO010000165         7271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10278_length_7271_cov_6.431125, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000165 DAOSOO010000000
VERSION     DAOSOO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_7271_cov_6.431125"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /gene="lysS"
                     /locus_tag="PLT86_10865"
     CDS             complement(<1..1423)
                     /gene="lysS"
                     /locus_tag="PLT86_10865"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT37152.1"
                     /translation="MEYVQLLIEQRKAKIEAIRALGVDPYPYRFDPANTTREIVADPE
                     RFSEQPVSIAGRLMSIRGKGKTAFAHIQDAEGRIQIYVRRDAVGETPYSIWNLLDIGD
                     IIGVKGPVFVTHTGETTVQVTEFVVLCKSIRPLPVVKEQMVEGERRVYDDIRSNVELR
                     YRHRSIDLIINPEVREVFRKRSQILQSVRRVLEDRGFIEVETPALQRVYGGANARPFK
                     THHNALDIDLYLKISPELYLKRLVVGGLERVYDLSKNFRNEGIDRTHNPEFTMLEAYQ
                     AYADFNDMMALTEAVYVESCLKVNGTTTISLQGREINLEPPWERVEMVEAIGKHAGIS
                     VENLSDEELLSMVRVHDPDAPAVLSRGMLIADLFEYRVEKELTGPVFVTNHPEETSPL
                     CKASRTRPGFIERFEPYILGYEIGNAYSEMNDPVRQRQLLEAQAAHREVDGEVPPVDE
                     DFLRAMEIGMPPTGGLGIGIDRMV"
     gene            complement(1946..2731)
                     /gene="nagB"
                     /locus_tag="PLT86_10870"
     CDS             complement(1946..2731)
                     /gene="nagB"
                     /locus_tag="PLT86_10870"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT37153.1"
                     /translation="MRIIIEKDYAGVSRRAADFVEALLRRKPNAVLGLATGSTPLGLY
                     RELIRRHKDEGLDFSGVTTFNLDEYYGLAPDHPQSYHHFMRVNLFDHINVPGDNINIP
                     SGTAKNIAKSCDDYERKIREFGGIDLQILGIGSDGHIAFNEPGSSLASRTRPKTLAPE
                     TIRDNARFFGEGEEVPKFAITMGVGTILESAACLLLATGKGKARILAEAVEGPVTSQV
                     TASALQLHPHAIVIADDEAASCLKRRAYYEHVERVSSGFGGES"
     gene            complement(2789..4225)
                     /locus_tag="PLT86_10875"
     CDS             complement(2789..4225)
                     /locus_tag="PLT86_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT37154.1"
                     /translation="MNEKPSAEVAAGVIGSRLVQALEEFLDSTSRRDEHLVKLLTVMA
                     KLAASAQTPLPGGAAHLEAADVSGVVAALEKIAERSVPAGSTAVQAPVPDITAVLEKQ
                     NQTNELLVRLITEQARRVSEAEMKQIPELSDQERWADLQIGSFDDKDIAFMKESEVFG
                     ALSDETLKVIRANATLEKYEPGKMIFVIGDPVSQVYIIKSGIVEICRPTDDPDKLNVV
                     AYLTAGDSIGEMSILISGDSRSSIARVPQGAEVLMIPHDVFMKLFKALPELALRLATV
                     FARRLKTSIKKERIQTHHRELQGSLEYFDLATIIQTLLSSDERTGVLTVTDEQQEPLA
                     DLYFEAGTMRYARYRQLLGEEAFYQLFQTENKKGSFSFKEGKFPEGFDQRAEVSVPGM
                     SLLFEAARLSDELKLLKEQIPDPDKVFKPKVDALEWTDDDNRTLANGIWNMLRRGASV
                     TELTENLPRSEYAIYAVLAEMLKSGQIE"
     gene            4479..5102
                     /locus_tag="PLT86_10880"
     CDS             4479..5102
                     /locus_tag="PLT86_10880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT37155.1"
                     /translation="MPDDPPVPENPHAAELKALLAEIARNEPGFFRSLAADVFKIPFG
                     YRWEDHPGVTIGKDVRIHPAARIELGSRSRLTIGDGSSIGPMAVLRPKDFHMIIGPNC
                     SVNDYTMLYDNLVLGRGVRIGAHSLIIPENHTFATRAKYIYEQPCEFKGVVFEDDVWV
                     GSNVVVLDGVRVGKGAILAAGCVVTRDVPPYAIVGGVPARVIKERPL"
     gene            complement(5114..6169)
                     /locus_tag="PLT86_10885"
     CDS             complement(5114..6169)
                     /locus_tag="PLT86_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37156.1"
                     /translation="MTSEERVLAACAFHRPDRVPRFDSFWDLPDAWRQEIGELKDLTD
                     IQIWAADETPFPSRKTVLKEQDGWLYERDGWGSTFRTRPGTYFREVLEVAIPEGVDPD
                     RVTFEDPASDRRYLMGRQTWSELRQELDSARREYCVFAKTGGPFLRTTLLRGEKQFLM
                     DIATDEVLARNLAEKVGSHLCDVGVESITRWGLTNTGIWIYDDVAFNEAPLFSPSAFE
                     RVFLPVYSQMISRYKAAGAKYVFFHSDGNINPFVDMLVDAGISGLHPLERRAGVDPFL
                     LRERYPKLVLAGGMCNTDILINGPVDRIEVEARKLIELARDGGLIIGTHSVSPEVPLE
                     HFRAYDRICRRYGVFTE"
     gene            6397..7230
                     /locus_tag="PLT86_10890"
     CDS             6397..7230
                     /locus_tag="PLT86_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37157.1"
                     /translation="MRLPEIDFGPHRISRLIVGGNPLRGNSHLTPERNAEMRDYYTTE
                     RVLDAWFASEKAGVTAMQSRGDQIVMDWVDRYRERGGTMHWIVQTASEWKGGDVADNI
                     RAVAEHHPIAIYHHGTRTDNLWKQGRIDDVHDCLKQIHDKGLLAGVGSHMPEVCEYIE
                     EHDWEVDFYMTCAYNLSREERESFLVNGSTGTEVYDDADRDRMTDFVLSTPKQCILFK
                     ILAASRKCATQESVKEAFAYAFRRIKPCDAVDVGVFQRDRDEIGLDAEYVREVTGFAA
                     S"
BASE COUNT         1536 a         2107 c         2050 g         1578 t
ORIGIN      
        1 tgaccattcg gtctatgcct atgcccaacc ctccggtcgg aggcatcccg atctccattg
       61 cgcggaggaa atcctcgtcg acagggggta cctcgccgtc cacttcgcgg tgcgccgcct
      121 gtgcctcaag aagctgccgt tgacgaacgg ggtcgttcat ctcggagtac gcgttcccga
      181 tttcgtaccc cagtatgtac ggctcaaatc gttcaatgaa ccccggcctc gttcgggagg
      241 ctttgcacaa cggcgaagtc tcctccggat ggtttgtcac gaaaacaggg ccggtaagtt
      301 ctttttccac tcggtattcg aacaggtcag cgatcagcat accccgcgaa agaacggccg
      361 gcgcatccgg atcgtgaact cggaccatgc tgagcagttc ctcatccgat aggttttcaa
      421 ctgaaatgcc ggcatgtttt ccgatagcct cgaccatctc gacccgttcc cacggaggtt
      481 cgagattgat ctcgcgtccc tgaagggaga ttgttgttgt tccgttcact ttgaggcacg
      541 attccacgta cacggcttcc gtgagagcca tcatgtcgtt gaaatccgcg tatgcctggt
      601 acgcctcaag catggtgaac tcagggttgt gcgtgcggtc gattccttcg ttgcggaagt
      661 tcttcgacaa gtcgtacacg cgttccaagc cgcctacgac caatcgctta aggtagagct
      721 cagggctgat tttgagatag aggtctatgt ccagagcgtt gtgatgcgtt ttgaacggcc
      781 gtgcgttcgc cccaccgtac acccgctgga gcgcaggggt ttcgacctcg ataaaaccgc
      841 gatcctcgag aacgcgcctt acgctctgca ggatctgcga ccttttgcgg aacacttcgc
      901 gcacttcggg gttgataatc aggtcgatgg agcggtgacg atagcggagt tcgacattcg
      961 aacgaatgtc atcatacacc cgccgctccc cctcaaccat ttgttccttc accacgggaa
     1021 gcggacgaat gcttttgcag aggacaacga actcggtcac ctggacggtg gtttcgcccg
     1081 tatgggtaac aaaaaccggc ccctttaccc cgatgatgtc accgatgtcc agcaggttcc
     1141 agatcgaata cggggtttcc cctacggcat ctctgcgcac gtagatctgt atccttccct
     1201 cggcatcctg aatatgcgcg aacgccgttt tcccctttcc ccgaatggac atcagccgtc
     1261 ctgcgattga taccggttgt tcggaaaacc gttcgggatc agcaacaatt tccctggtcg
     1321 tgttggcagg atcgaaccgg taggggtacg gatccacccc caacgcgcgg atcgcctcga
     1381 ttttcgcttt gcgctgctcg atcaacaact gcacgtattc cacggaatcc tccccggaat
     1441 cccagacagg agtcacggac tgcaacgtga aacgttagat acccatgaac actcgggagg
     1501 gttaaacaaa atgtaccgcc gagggccgga acacgccaac tgcacgtgga atcggcgtag
     1561 ttgccggcaa acgggaacag cgtgctcaga ggacgcaccc ggcaaacagc ggccatcaca
     1621 ccgaagccgc ccactgcaaa ttggacgtcg cagaacacga actcccgatc cgaaaagatt
     1681 ccaaccgggt gtctacgctc gtctcacttt gttgagcggg aaatccgcac tgtcaattcg
     1741 ggtggaaacc agggcgaaat gcgggcggga gatttcgttc aaaaaaagca ggggtgattc
     1801 ttcacaacag ccgctcccgc cgaaagcggc cgcggcgctc taattcgctc caaacgccgg
     1861 taattgccac gtgggaaccg gcggaacacg ccgcttcatc acgcgaagga taacccggaa
     1921 acgtcagcgg ctccacggac gagtatcagc tttccccccc gaatccgctt gaaacccgct
     1981 ctacatgctc gtaatatgcc ctgcgcttga ggcaactggc cgcttcgtca tccgcgatga
     2041 cgattgcgtg cggatgaagc tgcaatgcgc tcgcggtaac ctgcgaggtc acagggcctt
     2101 ccactgcttc cgccagtatt cgggccttgc ccttgccggt tgcaaggagc aggcaggcgg
     2161 cggactcgag tatggtgccg acacccatcg tgatggcgaa tttcggtacc tcttctcctt
     2221 ctccaaaaaa gcgggcgttg tcgcggatcg tctccggcgc aagtgttttg ggccgtgtgc
     2281 gagaggcgag gctcgatccc ggttcgttga aggcgatgtg gccatcactt ccaatgccga
     2341 gtatctgcag atcaatacca ccaaattccc ggatttttcg ctcgtaatca tcgcatgatt
     2401 ttgcgatgtt cttcgcggtt ccggaaggta tgttgatgtt atcgccgggc acgttaatgt
     2461 ggtcgaacag gtttacgcgc atgaaatggt ggtaactctg gggatgatcg ggtgcgagcc
     2521 cgtaatactc gtcgagattg aacgtcgtca caccggaaaa atccagaccc tcgtctttgt
     2581 gccgtcggat gagttcccgg tacagaccca gaggagttga gccggtcgcc agtccgagga
     2641 ccgcgttcgg cttgcgccgc agcagtgcct caacgaaatc ggccgcacgg cgcgagacac
     2701 cggcatagtc cttttctatg atgattcgca cgcgccaacc ttcttttctc tgagagcatg
     2761 cggctgccgc cgctgtgtga tgctcaccct attcaatctg accggatttc agcatttcgg
     2821 cgagcactgc gtagatcgcg tattccgacc gcggaagatt ctccgtcaat tccgtcaccg
     2881 acgcgcctct gcgcagcatg ttccagatac cgttcgccaa ggtccggttg tcgtcatcgg
     2941 tccattccag cgcgtcaacc ttcggtttga agactttgtc agggtcgggt atctgttcct
     3001 tgaggagctt gagctcatcc gaaagtcgcg ccgcttcgaa caacagggac atgcctggca
     3061 ccgatacttc ggctcgctgg tcgaaacctt ccgggaattt gccttctttg aacgagaagc
     3121 tccctttttt gttctccgtc tggaaaagct gatagaaggc ttcttcgcca aggagctggc
     3181 ggtaacgggc ataccgcatg gtccccgcct cgaaatagag gtcggccaaa ggttcttgct
     3241 gctcgtcggt aaccgtaagg acgcccgtcc tttcgtcgga ggacaacagc gtctggatga
     3301 tggtcgccag atcgaaatat tccagcgatc cctgaagttc gcggtgatgg gtttgaatgc
     3361 gctccttctt gattgacgtt ttcagccggc gcgcaaacac tgtcgcgagg cggagagcga
     3421 gctctggcaa tgccttaaaa agcttcataa acacgtcatg cggaatcatc aagacttccg
     3481 cgccttgcgg cacccgcgcg attgagctgc gggaatcccc ggagatgaga atggacatct
     3541 ccccgatgga atcacccgcg gttagatacg ccacgacatt cagtttgtcc gggtcgtcgg
     3601 tcgggcggca gatttccacg attcctgact tgatgatgta gacctgggag accggatccc
     3661 cgattacgaa aatcatcttg cccggttcat atttctccag cgttgcgttc gcacgaatga
     3721 ccttcaacgt ttcgtctgac agagcaccga aaacttctga ttccttcatg aacgcgatat
     3781 ctttgtcatc gaaggaaccg atttgaaggt cggcccaccg ttcctgatcg ctcaactcgg
     3841 gtatctgttt catctcggct tcggacacgc gccgcgcctg ttcagtgatc aaccggacca
     3901 aaagttcatt cgtctggttc tgtttctcca ggacggcggt aatgtcggga acgggagctt
     3961 gcaccgcggt cgagcccgca gggacggatc gttctgcgat cttctcgagt gcggcgacga
     4021 ctccagagac atcggcggct tccagatgcg ccgcgccccc gggcaaaggg gtctgcgccg
     4081 atgccgcgag tttggccatg acggtaagca gtttcacgag gtgttcatca cgcctcgatg
     4141 tggagtccag aaactcctcc aacgcctgaa caaggcgcga accgatcact ccggcggcaa
     4201 cttcggcact cggtttttcg ttcattgaga acctcccctg agccgttaac atcgtgctcc
     4261 ggctggcgca tccacaagcg aaccgcccgg caccagctgc ggacgggtcg ctgacgtggc
     4321 tccgtataat gcctaatgta cgcaaatagc gattgagtgg cgagtcaagg aggggaataa
     4381 gtcagcacgg ggggttgcgc ttcgatggcg ttggctcgtt cctgtaattt ccgatatgcg
     4441 aaccctcgtc ccagactttc tcacgcggag ttgaaccgat gcccgatgat ccgcctgtgc
     4501 ctgagaaccc tcacgccgcc gaattgaaag cgttgctggc tgagattgcc cgcaatgagc
     4561 ccggtttttt ccggtccctt gcggcggacg tgttcaaaat cccgttcgga taccgctggg
     4621 aggaccatcc cggcgttact atcgggaagg acgtacggat tcatcctgcc gcgcgcattg
     4681 aactgggcag ccgaagcagg ctcacgatag gcgacggaag ttccattggt cccatggctg
     4741 ttcttcgacc gaaggatttc cacatgatta tcggaccgaa ctgctcggtc aacgattaca
     4801 ccatgctgta cgataacctg gttctcgggc gcggcgtgcg tatcggcgct cactcgctca
     4861 taatcccgga aaaccacaca ttcgccaccc gcgcgaaata catctatgaa caaccttgcg
     4921 agttcaaagg ggtggttttt gaagacgacg tctgggtggg gtcgaatgtt gtggttctgg
     4981 acggcgtgag ggttgggaag ggcgcgattc ttgccgccgg ttgtgtagtc accagggacg
     5041 tgccgcctta cgcgattgtg ggcggcgtgc cggcgcgggt gatcaaagaa cgccctttgt
     5101 aatagatgca ccatcactcc gtgaatactc cgtaacgccg gcagatacgg tcgtaggcac
     5161 ggaaatgctc cagaggaacc tccgggctca cagaatgagt tccgatgatc agtccaccgt
     5221 cccgcgcaag ctcgatcagt tttcgcgctt ccacctcaat tcgatccacc ggcccgttga
     5281 tcaggatatc cgtattgcac attccccctg ccaggacaag ttttgggtag cgttcccgga
     5341 gcagaaaagg atccacgccg gcccgtcgct ccaacgggtg caggccgctg atacccgcat
     5401 ctacaagcat gtccacgaac gggtttatgt ttccgtctga atggaaaaag acgtacttcg
     5461 caccggcagc tttgtaccgc gaaatcatct ggctgtacac aggcagaaaa acgcgttcaa
     5521 aggcggacgg actgaaaagg ggagcctcgt tgaacgccac gtcgtcgtaa atccagatcc
     5581 ctgtgttcgt cagcccccat ctggtgatgc tttccacacc tacatcgcag aggtgtgatc
     5641 ccaccttctc cgccaggttc cgagcgagaa cttcatcagt cgcgatgtcc atcaggaact
     5701 gtttttcccc gcgcagaagt gtcgttcgca agaacgggcc ccctgttttt gcgaaaacac
     5761 aatactcacg ccgagcgctg tccagttcct gccgcaattc ggaccaggtc tggcggccca
     5821 tcagatagcg tctgtcgctc gcagggtctt cgaatgttac gcggtcggga tcgacgccct
     5881 ccggaatcgc aacttcaagc acctcgcgga agtaagttcc cggccgcgtc cggaacgtac
     5941 ttccccaccc gtcgcgctcg tagagccacc catcctgttc cttcagcacc gtttttcgag
     6001 aagggaacgg cgtctcgtcc gcggcccaga tctggatgtc cgtcaagtcc ttcagttcgc
     6061 cgatttcctg acgccacgca tcgggcaagt cccagaagga atcaaaccgc ggaactctgt
     6121 cggggcgatg aaacgcgcac gcggcaagca cacgttcttc cgaggtcatt atgccatttc
     6181 cctatcgaaa atcgaatgca cccgtgaaat caggcggaca ctccgggcat accgggtatg
     6241 cgcgccctga gtaccagttc cgttctgctg ctcgctcctg tcgtcgagcg ccggcaaagg
     6301 tgccgacggg acgtcgagag cgtacattca tttaccacgg gcatcaccag tggcacaagg
     6361 gaagacgacc tggatttcaa gaaggaggtt tgggcagtgc gtttgccaga gattgatttc
     6421 ggtccgcaca gaatttcccg ccttatcgtc ggtggaaacc ctctgcgcgg gaattctcat
     6481 ttgacgcccg aacggaacgc cgagatgcgc gattactata cgactgaacg tgttcttgat
     6541 gcgtggtttg cctcggaaaa ggccggcgtc acggcgatgc aatctcgcgg cgaccagatc
     6601 gtgatggact gggtcgaccg ttaccgggaa cgtggcggca cgatgcactg gattgtgcaa
     6661 acggcgagcg aatggaaagg cggcgacgtg gcggataaca tccgagccgt tgccgagcat
     6721 cacccgatag cgatttacca tcacgggaca cggaccgaca acctttggaa gcaagggcgc
     6781 attgacgacg tccacgattg tttgaagcag attcacgaca aagggcttct ggcgggggtg
     6841 ggatcacata tgccggaagt ctgcgaatac atcgaagaac acgactggga agtcgacttc
     6901 tacatgacat gcgcgtacaa tctcagccgc gaggaacggg agagttttct cgtcaacgga
     6961 agcacgggta cggaggtgta cgacgacgcg gaccgggacc ggatgacaga tttcgtgctt
     7021 tccacgccga agcaatgcat cctgttcaaa atcctcgccg caagccggaa atgcgcaacg
     7081 caggagagcg tgaaggaggc gtttgcgtac gcgtttcgcc ggatcaaacc gtgtgacgcg
     7141 gtggacgtgg gggtgtttca gcgggaccgc gacgaaatcg gattggatgc ggaatacgtg
     7201 cgcgaagtga ccggattcgc cgcttcatga cgtcgccacg aacgggacag tgatgcgatc
     7261 gaaagctgat g
//
LOCUS       DAOSOO010000166         7154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10494_length_7154_cov_5.500775, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000166 DAOSOO010000000
VERSION     DAOSOO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_7154_cov_5.500775"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1933)
                     /locus_tag="PLT86_10895"
     CDS             complement(149..1933)
                     /locus_tag="PLT86_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT37158.1"
                     /translation="MIQYDNPKPLLQRFKVIVPYVRPYTPYILGALGLTVVLTVLALL
                     PPLIMRALIDRVLTAGNWRILLPIIVAHALVPIMSGLISFFNTLVIAYAGRRLVFDVR
                     TQMYRHLLRLSMRFHGEMSSGAVMSRLMSDVNQVQNLIRENSITLVTDIVSFMFAGFV
                     TFSLNWKMAILLWIMLPLYVFNSRFFVKRIRRSNQRMRRTMDKIAGSLQERLSGTRRV
                     RAFGREEDETEQFLEQTEENLAHSMSGTIHSVSFSTASRLVWGFGTTILFVLGVYFAL
                     HGQMTYGSVTAFMAYVSQMFGPALRFTELANQLEQVMVSVDRIFEVLNTEPDVVEKPD
                     AVVLTSVVGHIQFDHVTFGYKPDEPVIKDVCLEIPPGKMVALVGHTGCGKTTITTLLM
                     RLWDVQEGSIRVDGHDIRDVTFASLRRSIGVVLQDPVLFNATIADNLRYGCPEATMDQ
                     VIEAAKAAEIHEMIMNQPEGYNTELGGESGIKPSVGEKQRMAIARAIITNPAVLILDE
                     ATSALDSQSEMLIQKALSRVMKNRTSLVIAHRLSTIVEADMIVVMDQGRVIETGSHAE
                     LLKAGGHYAGLFNQQRGTGKPAAVTAGS"
     gene            complement(1930..2871)
                     /gene="ispE"
                     /locus_tag="PLT86_10900"
     CDS             complement(1930..2871)
                     /gene="ispE"
                     /locus_tag="PLT86_10900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT37159.1"
                     /translation="MVPSITARAPAKLNLGLSIVGRRADGYHDLISVFHAVDWQDTLE
                     IVEIPSGIEFACSSCDGVPGAPQNLVFRALELVFQRFRITRGVAARLQKEIPAGAGLG
                     GGSSDAAATLRAVRRLFNLQGDEDSWLLLCQQLGSDVPFFWRGGTALVEGRGERLTPL
                     SIDERLWFVVVVPPIHISTAWAYGQLSKPSGGNQDYRDRIQRLRAGELSLSDFCREIP
                     NDFERVVEAAFPRIGEIRRALEASGAISARMSGSGSSVFGVYSKQEEAQRAAALFPSD
                     CLVRCVRGCPPPVIKCSEGGVFGNAVIGVCRAGCVVP"
     gene            complement(2884..3768)
                     /locus_tag="PLT86_10905"
     CDS             complement(2884..3768)
                     /locus_tag="PLT86_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37160.1"
                     /translation="MSVFNTITRVGLVGLLVPVVALLVNWSCSPQKSNSRTLARPVWE
                     CPVPPSSEVEAGPSASPLDDFVHLRWIVGSDDDLDGYFIHRRKSTDLIDTLFATVYLT
                     RDQLANRGSTIEWVDRNSRVGSVYYYVLFAFDKKGNRSARSDTVGFGTLEKPMIYGPS
                     QNQVVVDSRPVFQFGPRTTALYVQSYVVRVVSDSANSQVLWVSPRRPLLGYTSGERTT
                     IQYGNGGFTRRPFLNPGKYRWRVDFQGSYPFVDIDAPCGCVYDTVTCPGASPTLPRPE
                     DLSFISSQSAWVPFTVAP"
     gene            complement(3743..4756)
                     /locus_tag="PLT86_10910"
     CDS             complement(3743..4756)
                     /locus_tag="PLT86_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37161.1"
                     /translation="MKIQAENAARAVLFGAFMAAVAKASTPSADAFLVAPVSAREVSL
                     GQATVGSVNDATAFHRNPAGLGYVRSFDAAASYSRVFGSLATHQMVAFALPVTELVTA
                     GAGWVRMGVDDIPVYPSLSSYPTPYERESYARLGSTGSIGFSQNAFFLTFARMHRFNV
                     DFGWQYLTLPVEMPVGVTLKYLTVAAGDTASGQGLGLDVGIQSRFSLSRALDVPSAGM
                     LDIGVTLVNAGRTKITWDTATRREDVQSMAVRFGAAYEQPLPRINSAITVAAAKIGDG
                     GAWGVEYSFARQIMLRVGDSGIGEGSFAGGAGFRWRNVDLDYALQRHPLGTSHRVSVQ
                     YHH"
     gene            complement(4753..>7154)
                     /locus_tag="PLT86_10915"
     CDS             complement(4753..>7154)
                     /locus_tag="PLT86_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT37162.1"
                     /translation="WVNNDFYFTQSIIRAAFAQSRERQTLGSIISAAKTDYLMRYGGG
                     NADRGSIPHSIVHEYNLIGDPGLQITPPVNRAALVPNVRTPALGQQIAVSGSLPEPIN
                     GTATITLVDDYQFEVALGAQVPVSGGTFSAVFNAPTSLVGTGLTLKTYVAPAATGSTA
                     DWAGYARMAVADILVDSVIVERTQPDSFYVSSTVYGTVGIQAVVCSLVVNSAGTYDNV
                     PMLRVGSSSWFRTVRALDISGIPSDDLYAFAQPVIKAVDGNGKVTIRRDISPIYPNAR
                     AALRVVSAELVGASRGELGVTIANRGTIPSDSVVARAWIVDDNGARTLLSETRLTPLA
                     SQTNPISDAGDVVIAKPTATQGNGDEILPQYARASFWLPDSLLRGQSPGKRILVAAEA
                     ADGRAVRDSTTFTVPPSLGVYSSASQYALRVVVPSGKFSAEVVPGTFTAETLVTVSQT
                     TAPAKTTQPDVDPPGAKSGRLADACEVKWLAPVVPLPGASPSLTLHLDKTDTDAVSAL
                     AQGRLRVAYWNANQQQWELIDQQYHTWASDSSATTVPADRAGIYVFVIVDDTTPPDIE
                     ISARGQQFGEGAYVNPFTKFVALLEDRNGIATGPGAVKVWLDDVALPDSLITFPVQVA
                     SATALPVTITPPLLTPREAPYRLRVSARDAAGNVGESTVNFRVAGQTLVDFYGNFPNP
                     FGSQGTIFAFSFSAQISEVTFRIYDLAGRLVLKFSNYDLSALYPDDSPSLHQSTSPYR
                     LLDRNGLPLVAPSYHELEWSGRNARGEFLANGVYFGIITVRDEAGDEVAKRIFKMVKA
                     E"
BASE COUNT         1561 a         2101 c         2056 g         1436 t
ORIGIN      
        1 ccccgacaag ggtgtatgaa agatagggaa ctgcgcgttg gcacggcaag caaaagaagc
       61 cgttgtactt tgctcgccgg gagttggtct gcgtctgcgc ggcgcgcgtt tcgcgaggta
      121 ttgttcccgc ggaggccgaa gaaaggcgtc acgaaccggc ggtaaccgcg gcgggttttc
      181 cggtgccgcg ttgctggttg aagagccctg cgtaatgccc ccccgccttc aacaactcgg
      241 cgtgcgaacc tgtttcgatc acccttccct gatccatcac cacgatcatg tcggcctcga
      301 ctatcgtgga caggcggtgc gcgatcacga ggctcgtccg gttcttcatg acccgggaga
      361 gcgctttctg gatcagcatt tcactttgtg aatcgagcgc cgaagtggcc tcatcaagaa
      421 tcagcaccgc cggattggtg ataatagcgc gcgcgatcgc cattcgctgt ttctcgccga
      481 cggatggttt gatcccactc tctcccccga gttctgtgtt gtagccctcc ggctgattca
      541 tgatcatctc gtgaatttcc gcggcctttg ccgcttcgat cacctggtcc attgttgcct
      601 cgggacaccc gtaccgcagg ttgtcggcta tcgtggcatt gaatagcacg ggatcctgca
      661 gcacaacccc aatgctcctt cgcaggctgg cgaaggttac gtcgcggata tcgtgcccat
      721 caacgcggat agagccttcc tggacgtccc acaggcgcat gaggagtgtg gtgatggtgg
      781 tcttgccgca tccggtatgc cccacaagag ccaccatttt ccccggagga atctcgagac
      841 aaacatcctt gatgactggt tcgtcgggtt tgtatccgaa cgtaacgtgg tcgaactgaa
      901 tgtgaccgac caccgaagta agaacaacgg catccggttt ttccacgaca tcgggttcgg
      961 tgttgaggac ctcgaagatg cggtcgaccg acaccatgac ctgttcaagc tgatttgcga
     1021 gttcggtgaa cctgagggcg gggccgaaca tctgcgagac gtatgccatg aatgcggtaa
     1081 cagagccgta cgtcatttgc ccgtgcagcg cgaaatagac accgaggacg aagagaattg
     1141 tcgtaccgaa cccccagaca aggcgggatg cggtggaaaa cgacacactg tgaatagtgc
     1201 cggacatcga atgggcgagg ttctcttcgg tttgttcgag gaactgctcg gtctcgtcct
     1261 cttcgcggcc aaaggcccgt acgcgacgcg ttccggagag ccgttcctga agcgaccccg
     1321 cgattttgtc catggtacgc cgcattctct ggttcgagcg gcgaatccgt ttgacaaaaa
     1381 accgcgagtt gaagacgtac aggggaagca ttatccacag tagaatcgcc atcttccagt
     1441 tcagcgagaa cgtcacgaat ccagcgaaca tgaagctgac gatatctgtt accagggtga
     1501 tgctgttctc tctgataagg ttctgcacct ggttcacgtc gctcataagg cgcgacatca
     1561 ccgcgccact ggacatttcc ccgtggaatc gcattgagag gcgcaacagg tggcggtaca
     1621 tctgcgtgcg gacatcgaat acaagccttc ggcccgcgta cgcgatcaca agcgtgttaa
     1681 aaaaggagat cagcccggac atgatcggga caagagcgtg ggcgacgata atcgggagaa
     1741 ggatgcgcca gttgcccgcg gtgaggaccc tgtcgatgag cgccctcatg atgagcggag
     1801 gcagcaacgc gagaacagta aggaccaccg tcaacccgag agcacccagg atgtacgggg
     1861 tgtacgggcg tacgtacggc acaattacct tgaaacgctg gagcagaggt ttgggattat
     1921 cgtattgaat catggaacaa cgcatcctgc gcgacagacg ccgattacgg cgtttccgaa
     1981 gacaccaccc tcagagcatt tgatcacggg aggagggcat cccctcacac atcttacgag
     2041 acaatctgac ggaaagagcg cagcggcccg ctgtgcttcc tcttgttttg aatacacacc
     2101 gaacaccgaa gacccggacc cactcattcg cgcggatatg gcccctgagg cttcgagcgc
     2161 gcggcgtatc tcccctattc gcggaaaggc tgcttcgacc actctttcga agtcgttcgg
     2221 aatctcgcga cagaaatcag agagcgaaag ctcgccagct cgaagacgct ggatccggtc
     2281 ccggtagtcc tgattacccc cggacggctt tgagagctgg ccgtacgccc acgccgtcga
     2341 gatgtgaatc ggcggtacca ccaccacgaa ccagagcctc tcgtcaattg agagaggtgt
     2401 gagccgttct cctctccctt ccaccagagc cgttcctccc cgccagaaaa aaggaacgtc
     2461 cgagcccagt tgctggcaga gcaacaacca ggagtcctcg tcgccctgga ggttgaagag
     2521 acgacgcact gctcgcaacg tggccgcagc atcggaactg ccgcctccga gtcccgcacc
     2581 ggccgggatt tctttctgga gtctggccgc aacgccacga gtaatgcgga acctctggaa
     2641 caccagttca agcgcccgga aaacgaggtt ctgcggcgcg ccgggaactc cgtcgcacga
     2701 agagcaggcg aattcaatcc cggacgggat ttccacaatt tcgagagtgt cctgccagtc
     2761 cactgcatga aaaaccgaaa tcaggtcgtg gtacccgtcg gcgcgccgtc ctacgatgga
     2821 aaggccgagg ttgagtttgg caggagcgcg agcagtaatc gaaggcacca ttactggaga
     2881 ccgtcagggt gccacggtga acggaaccca ggctgattgt gagctgatga acgagagatc
     2941 ctccggacgt ggcagggtgg ggctcgcccc cgggcaggtc accgtgtcgt acacgcagcc
     3001 gcagggagcg tctatatcga cgaaggggta acttccctgg aaatcaacac gccaacgata
     3061 cttcccggga ttgaggaacg ggcggcgggt gaaaccgccg ttgccatact gaatcgtcgt
     3121 tcgttccccg gaggtatacc ccagcagcgg cctccgcgga cttacccaga gaacctgcga
     3181 attggccgaa tccgatacca cgcggacaac ataactctgc acatagagag ccgtggtgcg
     3241 cggaccgaat tgaaaaacag gacggctgtc aacgacgacc tggttttgcg atggtccata
     3301 aatcattggt ttttcgaggg tcccgaatcc gacggtatca cttcgagcgc tgcggtttcc
     3361 tttcttgtcg aatgcgaaga gaacgtagta gtacacgctc cccactctcg aatttcgatc
     3421 cacccattcg atggtggagc cacggtttgc caactggtcg cgagtcaggt acacggttgc
     3481 gaaaagcgtg tcgatgaggt cagtgctttt gcgccggtgt atgaaatacc cgtcgagatc
     3541 atcatcgctt ccgacgatcc atctgaggtg cacgaagtca tccagtgggc tggcgctcgg
     3601 cccggcttcg acttccgaac tcggaggaac cggacactcc cataccggtc gcgcgagcgt
     3661 ccgcgaattc gacttctgcg gactgcacga ccagttcacc agaagcgcca ccacgggcac
     3721 aagcaacccg accagtccca ccctagtgat ggtattgaac actgacacgg tggctcgtgc
     3781 ccagagggtg ccgttgaagc gcataatcga gatcaacgtt tctccacctg aaaccggccc
     3841 ctccggcgaa tgacccttct ccaattccgc tgtctcccac ccgaagcatg atctgccggg
     3901 caaacgagta ttccacaccc cacgcgccac cgtcgccaat cttggcagcg gccacggtga
     3961 ttgcgctgtt gattctcggc aatggttgtt cgtaggccgc cccgaaacga actgccatcg
     4021 attggacatc ctctcgtctg gtcgcggtat cccaggttat cttagtccgc ccggcgttca
     4081 caagcgtaac cccgatgtca agcattcctg ccgaaggaac atcaagggcg cgcgaaagcg
     4141 aaaagcggga ctggatacca acgtccagtc cgagtccctg gcccgacgcg gtgtctccag
     4201 cggcgacggt gaggtatttc agagtaacac ctactggcat ttccaccggc agcgtgaggt
     4261 attgccaacc gaagtccacg ttgaaccgat gcatccttgc gaacgtgaga aagaacgcat
     4321 tctgactgaa gcctatcgag ccagttgacc ccagtcgtgc gtaagattcg cgctcatagg
     4381 gtgtcggata ggaactcaga gacgggtaca cggggatatc atccactccc attcgcaccc
     4441 accctgctcc agcagtcaca agctccgtta caggcaacgc gaacgcgacc atctgatggg
     4501 tggcaagact cccgaacaca cgtgaatacg aagccgcggc atcgaacgaa cgcacgtagc
     4561 cgagacctgc gggattcctg tggaatgcgg tggcatcgtt cacagaacca accgtggcct
     4621 gccccagcga gacctctctt gcggaaaccg gcgcaacgag gaatgcgtcc gcagaaggag
     4681 tggatgcttt tgccacagcc gccatgaagg caccaaaaag cactgcacgc gcagcgttct
     4741 ctgcctggat tttcattccg ctttcaccat tttgaagatt cgcttcgcaa cttcgtcgcc
     4801 cgcttcatcg cgcaccgtta ttatcccgaa gtatacgcca ttcgcgagaa attcacctcg
     4861 agcattccgg cccgaccatt cgagttcgtg gtagcttggc gccaccaacg ggagcccgtt
     4921 ccgatccagc aggcggtacg gacttgtcga ttggtgcaac gatggggaat cgtcggggta
     4981 aagcgcggac aagtcgtagt tgctgaattt gagaacaagc cttcccgcca gatcgtagat
     5041 gcggaaggtc acttcactta tctgcgcgga aaatgaaaag gcgaagatag ttccctgaga
     5101 tccgaaagga ttcgggaagt tcccatagaa gtccacgagc gtctggcccg cgacgcggaa
     5161 attcaccgta ctttcgccta catttcctgc ggcgtcccgt gcgctcactc gcagtcggta
     5221 cggagcctca cgaggagtaa gaagaggcgg agtaatcgta actggcaacg cagtggcgga
     5281 cgcaacttgc acggggaagg ttatcaggct gtccggcaat gccacatcgt ccagccagac
     5341 tttgacggca ccgggccctg tcgcaatgcc gtttctgtcc tccaacaagg ccacgaattt
     5401 cgtgaacggg ttcacgtacg cgccttcgcc gaactgctgt ccgcgcgcgc ttatctcgat
     5461 gtccggcgga gttgtgtcgt ctacgatgac gaacacgtag atgcccgccc ggtccgcagg
     5521 aaccgttgtg gcgctgctat ccgaagccca tgtgtgatac tgctggtcta tcagctccca
     5581 ttgttgttga tttgcgttcc agtacgctac ccggagacgc ccctgagcga gtgcactcac
     5641 cgcgtcggta tcggttttgt cgaggtgcaa tgtaagtgag ggagaagcgc ccggtaatgg
     5701 aaccacgggc gcaagccatt tcacctcgca cgcatcagcc agccgtcccg attttgcgcc
     5761 gggcggatcc acgtcgggct gcgtggtttt tgccggggct gttgtctggg agaccgtcac
     5821 gagtgtttct gcggtgaaag tccctggcac cacttcagcc gagaatttgc ctgacggaac
     5881 gacgacgcgc aacgcatatt gcgacgcgga ggagtacaca ccgagggatg gcggaaccgt
     5941 gaaggtcgtc gaatctcgaa cagctctccc atccgccgct tcagccgcga caaggattcg
     6001 ttttcccggg ctctgtccgc gcaagagcga atctggaagc cagaacgaag ctctcgcgta
     6061 ttgcggcagt atttcgtccc cattcccttg tgtcgcggtc ggtttcgcaa tcaccacatc
     6121 cccggcgtca gaaatggggt ttgtctgcga cgcgaggggt gtgagacgtg tttctgacaa
     6181 cagggtgcga gctccgttgt catcaacaat ccatgctctg gcaaccacag agtcggaagg
     6241 tattgttcct ctgttggcga tggttacgcc gagttctccc cgcgacgcgc cgaccagttc
     6301 cgcggacacg acccgtaacg ccgctcgcgc gttggggtaa atgggagaaa tatcgcgccg
     6361 gatggtaact ttcccgtttc cgtccacggc tttgattacg ggctgagcaa acgcatacag
     6421 gtcgtcagac gggatcccac tgatatcgag ggcgcgaaca gtgcggaacc acgacgacga
     6481 accgacgcga agcatcggga cattgtcgta cgtccccgcc gaattcacga cgagagaaca
     6541 aaccacggcc tgaatgccaa cggtaccgta gaccgtagaa ctgacgtaga acgagtctgg
     6601 ttgcgtcctt tccactatca cgctgtcgac gaggatgtcg gcaactgcca tcctcgcata
     6661 ccctgcccag tcggcagtgc ttccggtggc tgccggcgca acgtacgttt tcagcgtgag
     6721 gccggttccc acgagggaag ttggcgcgtt gaaaaccgca ctgaacgttc ctcccgagac
     6781 gggaacctgc gcaccaagag ccacttcaaa ctgatagtca tcaacgaggg ttatggtcgc
     6841 agtaccattg attggttccg ggagtgagcc gctcaccgcg atctgttggc ccaatgcagg
     6901 tgtgcggacg ttcgggacca gcgccgctct gttgacgggt ggcgtaatct gaagccccgg
     6961 gtcaccgatg aggttatact catgaacgat cgaatgcgga atcgaacctc gatcggcgtt
     7021 tccgcctccg taccgcatca gataatccgt tttcgctgcc gatattatcg aaccgagggt
     7081 ctgtcgttcc cgggactggg cgaatgccgc acgaatgatc gactgggtga agtagaaatc
     7141 attgttcacc cagc
//
LOCUS       DAOSOO010000167         7041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10734_length_7041_cov_5.816490, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000167 DAOSOO010000000
VERSION     DAOSOO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10734_length_7041_cov_5.816490"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..896
                     /locus_tag="PLT86_10920"
     CDS             99..896
                     /locus_tag="PLT86_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37163.1"
                     /translation="MKCHFAGTIGFLGLCVFATLLVRGESRAETGLTGSTALLYVPVA
                     DVVPNERFSFGGLIADYQAFPSKQFSYFRANKPSAYFSGYATIGYIPRLEITVRGSGM
                     PSAMGPAGTGPFYTDGMISMQALLLRGRGRVPSVGAGFQDMYGFMIFNALYGVVTWSV
                     PAEHGNAVITGGWAVDWYDRNRGTADVNYIVPHVINGFFAGGAYPIRKWVDGIAEYDS
                     RSLNVGMRFHPVSWCTLDLAAVRWGLDRMARGQVAGFTAHLCFSGHL"
     gene            990..3008
                     /locus_tag="PLT86_10925"
     CDS             990..3008
                     /locus_tag="PLT86_10925"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOT37164.1"
                     /translation="MHDEKKDFRVYVDVVYRKKWLILFIFLIVFLTTLFFVRRQAPSY
                     TSSSSIYIESGNPTAMAFSLSSDAQGKRPIEFFMGILESRSFRESVRDAVRDHAISRG
                     MPPIQATDLSAKAIESLQVKRSKNEGFYTLTAESESPDIAYATDSLTVGLFIERCILY
                     ARQQTSGMTAFVAMQFDSARARLERAEDNLQAFRKQHNLIQVGGDPATDPSLPIDYVR
                     LVEGYYDARQERQTAQAALEAAEEASRLLRINLDTLATDKLRRALPALDLAEAEERTR
                     KARTDLQLKELQEQSFLSQLQAYEASHPGLSDVSIGYLRLVRERDIYVRLSDLLLERR
                     EELRVKSASETGGVRVVDAPSEATPKPSRAPLILVLSAIIGLSLGVGAAFLLEMMDTS
                     VRTVGDITEALEVPTIGTIPSIGVAPGGKRRRESERRRVLISDGNPKDPVAEAYRTLR
                     TSILYSSGDQPIRSLVISSAGQAEGKSITVANLAIVCAQMGQRTVVVDADLRRPVQHI
                     LFELERDEGLTEALVHRHELAEVAKPTNIENLDVVTCGVIPPNPATLLGSAAMGERLQ
                     ELVQHYDIVLIDTPPVIVVTDAVLVARQTDGVLLVVRCNSTPREAARHTADTLRTANI
                     PLIGAVLNDIDVTRRYGGYHYYNYYYHYYYGGYYGSSDGTGDTKTAKT"
     gene            3265..5052
                     /locus_tag="PLT86_10930"
     CDS             3265..5052
                     /locus_tag="PLT86_10930"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOT37165.1"
                     /translation="MFQPVRAIAIFAAVSLLAAGASARPNTIGIGEIHEGMRGYGLTV
                     FSGVRIDTFQVEVLAVLRNQSGPADAIVARVSGGPLSETGVAQGMSGSPVYLDGRLAG
                     AVAWTSTFSKSPIAGITPIESMLEIPSRPMTPPPPDRYAFSERGAPRSSLNAVPRLAD
                     AGALAGTEMMPIKTPVAVAGADARALAFLQNILEPYGMTVLQSAGGTIGSDSLRNARL
                     EPGASLGVSLVRGDINVAGIGTVTWADSTTVIGFGHPMFSKGHVDLPMSLAYVHFLWP
                     SQVVSYKVASQGPVVGALRQDRGSGIAGVLNERPDMLPVEIKISGGPRPRVVHYEVVR
                     DIDLSPQLVTFGVVASLSELEALGIPASVEMTQIIQIEGRAPVRRKNFFTDFGGLMLA
                     AQAGAEPLDALARNPFAPVRIERVAYEISFRENIEAAFIKGLEIPRRVIRPGEPLIVR
                     AIFQTYLGRQFEKTLSLPISPETPDGVYILRVGGADAAQRWTVARSPGRFIPDNLDHL
                     IRLLNYEERNDNLTLEVVNRDIGMTVDDQELPSLPPSVFELLRSAVPGGRIGPILGTP
                     LVRKQEPMGLYVLGSQEISVAVYRFARPR"
     gene            5069..>7041
                     /locus_tag="PLT86_10935"
     CDS             5069..>7041
                     /locus_tag="PLT86_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37166.1"
                     /translation="MVRKLLFVLVLAASVMPVHKSGAVTPQRWFLSSAQDMESGDVEG
                     LSVADDGTLSLAPVLEDVGETEEMFVWALAEDRQGRLFAATGNQGRVFIKEGTQTPRL
                     FFETPQTQIQSLVIDPQGNVYAGAAPDGVIYKILPDGRGNVFCRTGETYVWDMEFGVD
                     GSLYAATGTRGIVLKIDRDGKIVDKVLDTSDPHLMTLVPDGRGGFYAGTDGNGLVYAI
                     ENDRSRLLFAAAEREIHTLAVGPDGRVYAGATGARNPGEQSTERSPSGAVYRLEPSGA
                     ATKLWTAPYPLVLSIVPYDSSRVLVGVGPRGVLYLLSTDGRTQRVADTGESQPACFLK
                     RSSGEVLVGMGNSGKIKRLTTKPGTRGVFVTPARNAGYVAQWGRVEIRGVTPPGTRVH
                     VESRTGNKSDPATEWSAWQPLFGEEQDRIQSPAAQYAQLRLTLTRAENAPSPQVSSIG
                     ITSLQVNLRPEISMVSVQPVRSTQTGSQSSSQQGESSSDQSSTPRTTTPRGAPTKRSL
                     VSVRWNVTDPNGDDLVYSLYFRRVSETTWHLLDELLTRNSYVWDTEGAPEGMTVLKVV
                     ASDQPSNPWQTALSAERESAAFPVDYSGPVVSELTASPRPDGSVVLRGVGSDTVGPIN
                     DGAYSVNSGEWRPFFPVDGIFDGKREAFEVLTEA"
BASE COUNT         1373 a         2021 c         2190 g         1457 t
ORIGIN      
        1 attattgtgc agaaaaggat actgacattc tcagcggttg ttgcgacgct cagcctggca
       61 attcaggctc tgacgctgta tttcctggtt cagaagcttt gaagtgtcat ttcgcgggca
      121 cgatcggttt tcttggtctg tgcgtttttg cgaccttgct ggttcgtggt gaaagccggg
      181 ctgaaacagg cctgaccggc tcaacggcgc ttctctatgt tccggtggcg gacgtcgttc
      241 cgaatgaaag gttctcgttc ggcggtttga tcgcggatta ccaggcattc ccgtcgaagc
      301 agttctcgta ctttcgggcg aacaaaccga gcgcgtattt cagtggttac gcaacgatcg
      361 gttatatccc gcggctggaa atcacggtcc ggggaagcgg aatgccgtcc gcaatgggtc
      421 ccgcgggtac cgggccgttc tacacggacg gtatgatcag catgcaggcc ctgttactcc
      481 ggggcagggg acgcgtgccg tcggtcggtg ccggtttcca ggacatgtac ggcttcatga
      541 tcttcaatgc cctttacggc gttgtgacat ggtctgtgcc cgcggaacac gggaatgcgg
      601 tgattacggg aggatgggca gtagactggt atgacaggaa ccgtggcact gcggacgtca
      661 actacattgt ccctcacgtc atcaacgggt tctttgcggg tggtgcgtac cccatccgga
      721 agtgggtaga cggcatcgcg gagtacgact cgcgatccct gaacgtcggg atgcgatttc
      781 accccgtttc gtggtgtact ctcgatcttg ctgccgtccg gtgggggctt gacaggatgg
      841 cgcgcggtca ggtcgccggt tttacggcac atttgtgctt tagcgggcac ctgtagcgga
      901 gtctttcgcg gagcagcagg tcgtgtttca gggttccttc ttcgcgggga acgggggagt
      961 cggatccggt atcagggagc aacgcgataa tgcacgatga gaagaaagac ttccgcgtct
     1021 acgtggatgt cgtctaccgg aaaaaatggc tgatcctctt tatctttctc atcgtgtttc
     1081 tgaccacgct ttttttcgtg cgcaggcaag cgccgtccta cacctcctcc tcctccattt
     1141 atatcgaaag cggcaatccc acagcgatgg ctttctcgtt aagttcggac gcacagggaa
     1201 aacgtccgat agaatttttc atgggcatcc tcgaaagtcg aagttttcgt gagtctgttc
     1261 gcgatgcggt ccgcgatcac gcgatttcgc gagggatgcc gcccattcag gcgacggacc
     1321 tgagcgcaaa ggcgattgag tcccttcagg tgaagcgcag caaaaacgag gggttttata
     1381 cactcactgc agagtcagag tcccctgata tcgcatatgc gacggatagc ctcacggtcg
     1441 ggctttttat cgaacggtgc atcctgtacg cccggcaaca gacaagcggg atgacggcgt
     1501 ttgtagcgat gcagttcgac tccgcgcgtg cgcgtctgga acgtgcggag gacaatctcc
     1561 aggcgtttcg caaacagcac aatctcattc aggttggcgg cgatcccgca acagatccga
     1621 gcctgcctat agactacgtc cgcctcgtcg aagggtacta tgacgcccgg caggagcgcc
     1681 agacggcgca ggccgctctc gaggctgcgg aagaagcatc ccgtcttctc cgcatcaatc
     1741 tcgacacact ggcgactgac aaactgcggc gagccctccc cgcgctcgat ctggcggaag
     1801 cggaggagcg tacccgcaag gcacgaaccg acctccagct caaagaactc caggaacaga
     1861 gctttctttc tcagttgcag gcatacgaag cctcccatcc cgggctttcg gacgtaagca
     1921 ttgggtacct cagactcgtc cgcgaacgcg atatctatgt gcggttgtcc gatctactgc
     1981 tcgaacggcg ggaggaactg cgcgtgaaat ccgcctcgga aaccggcggc gtgcgcgttg
     2041 tggatgcacc gtcggaagca acaccgaaac caagcagggc tcctctgata ctggtcttat
     2101 ccgcaatcat cggcctttcc cttggcgttg gggccgcgtt ccttctggag atgatggata
     2161 cgagtgttcg aacggtaggc gacatcacgg aagctctgga ggtccccacc atcggaacga
     2221 tcccgtccat cggcgtcgcg ccgggaggca agagaaggcg tgaatcggaa cgccggcgcg
     2281 tgcttatttc cgacggaaat cctaaagatc ccgtcgccga agcataccgg accttgcgaa
     2341 cgagtattct ttattcttcg ggagaccaac cgattcggtc ccttgtcata tcaagcgcgg
     2401 ggcaggccga aggaaagtcg attaccgtcg ccaatcttgc catcgtctgc gcgcagatgg
     2461 ggcaaaggac agtcgtcgtc gacgcagacc tccgccggcc ggtgcaacat attcttttcg
     2521 agcttgagcg cgatgaaggg ctcaccgagg cgctcgtgca ccggcatgag cttgccgaag
     2581 tagcgaagcc aacgaacatc gagaatctcg atgtagtcac ctgcggcgtc attcccccga
     2641 acccggcgac gctgctcggg tccgccgcca tgggggaacg gcttcaggaa ctggtgcaac
     2701 attatgatat cgtccttatt gacacgccgc ccgttatcgt ggtgaccgac gccgtcctgg
     2761 tggcccgaca gacggacggc gttcttctgg tggtccgttg caactccacg ccgcgcgaag
     2821 cggcgcgtca caccgcagac acgctgagga cggccaatat cccgctgata ggtgccgtgc
     2881 tgaacgatat agacgtaacc cgtcggtatg gcggctacca ctactacaac tactattacc
     2941 actactatta cggcggctac tacggaagct ctgacggcac cggagacaca aaaacggcga
     3001 agacctgaaa cctgcgcccg ggtcacgacg tcgctcgtgt gacgaatccg agtttgcccc
     3061 gggtgttgtg gccttccgca accgttgttt cataatgcgc attttcaggc gtttcctgac
     3121 gacaacaggc gttaattcac ccccgggcgc cttcctcctt atattctgga tacaatcccg
     3181 cctgatctga cactgagacg acactcgcgt gttttcggca gggttgattc cttgcgcatt
     3241 tgtctcaaaa gggtaccgac aagaatgttt caaccggtgc gagcgatcgc gattttcgct
     3301 gcagtgagtc ttctcgcggc gggtgcttcg gcgcgcccca acaccatcgg aattggagaa
     3361 atccatgagg ggatgcgcgg gtacggactc acggtgtttt ccggagtacg gatagacacc
     3421 tttcaggtgg aggttctcgc cgttctgcgg aaccagtcag gccccgcgga tgctatcgtc
     3481 gcgcgagtga gtggaggtcc actctccgaa accggtgtcg cccaggggat gagcgggtca
     3541 ccggtgtacc ttgatgggcg gcttgcgggt gccgtcgcgt ggacaagcac cttttcaaaa
     3601 tcgccgatcg ccggaattac cccgatcgag agcatgctgg aaatccccag ccggccgatg
     3661 actcctcctc ctccggatcg gtatgcgttt tcggaacgcg gtgccccccg gtcgtctctg
     3721 aatgccgttc cccggctggc ggatgcgggc gcccttgcgg ggacggaaat gatgccgatc
     3781 aaaactcccg tggccgttgc gggcgcggac gcacgggctc tcgcgttcct gcagaacatt
     3841 ctcgaaccct atggtatgac ggtgctgcag agcgccggcg gcacgattgg atcagactcg
     3901 ctgaggaacg caagactgga acctggcgcg tcactgggtg tcagcctcgt gcgcggtgac
     3961 ataaacgtag ccgggatcgg aaccgttacg tgggcggaca gcaccaccgt tatcggtttc
     4021 gggcacccga tgttttccaa aggtcacgtc gatctcccca tgtcacttgc gtacgtgcat
     4081 ttcctgtggc caagccaggt ggtttcctac aaggtggcct cgcaagggcc tgtggtaggg
     4141 gcgctgcggc aggatcgggg aagcggcatc gccggggttc tgaacgaacg accggacatg
     4201 ctgccggtgg agatcaagat ttcgggaggc ccccggccac gcgtcgttca ctatgaagtg
     4261 gtgcgggaca ttgatctttc gccacaactc gtgacgttcg gtgtcgtggc aagcctttct
     4321 gaactggagg cgctgggtat tcccgcatcc gtcgaaatga cccagatcat ccagatcgaa
     4381 ggccgggcac ccgtgcggcg gaagaacttc ttcacggatt tcggggggct catgctggcc
     4441 gcgcaggccg gagccgaacc actcgacgcg cttgcgagga atccgtttgc gccggtgcgt
     4501 atcgaacggg tggcgtatga aatttctttc cgtgagaaca tagaggcggc cttcatcaag
     4561 gggctggaga ttccccgccg ggtgatccgg ccgggtgagc cgctgattgt ccgggcgatt
     4621 tttcagacct atctcggaag gcagtttgag aaaacccttt ccctcccgat ttctccggaa
     4681 acccctgacg gcgtgtacat tctccgggtg ggtggcgccg acgcggcgca acgctggacc
     4741 gttgcgaggt cgccgggacg tttcatcccc gacaatctcg accacctgat tcgccttctg
     4801 aattacgaag aacgtaatga caatcttact ctcgaagtgg tgaaccggga tatcggaatg
     4861 acggtggacg accaggaatt gccgagcctt ccgcccagcg tgttcgaact tctgcgcagt
     4921 gcggttcccg gaggaagaat cggccccatt ctcggaacgc cgctcgttcg caagcaggag
     4981 cccatggggc tgtacgttct gggttctcag gaaatcagcg tcgcggtata ccggttcgcg
     5041 cggccacgct agtccgacgg agaagagaat ggttcggaag cttctgttcg tattggttct
     5101 tgccgcgtct gtgatgcctg ttcacaaaag cggcgccgtt actccccaga ggtggttcct
     5161 ttcctcggcg caggacatgg aaagcggcga cgtggaaggc ctttccgtgg ccgatgatgg
     5221 gacactgagc ctcgcgccgg tgctggagga cgtgggagaa accgaggaga tgttcgtgtg
     5281 ggctcttgcg gaggatcggc agggccgact tttcgccgca acgggaaatc agggacgcgt
     5341 tttcatcaag gagggaaccc agacaccccg cctgtttttc gaaactcccc agacgcaaat
     5401 ccaaagcctt gtgattgacc cgcagggcaa cgtctatgcg ggagcggcgc cggacggcgt
     5461 gatctacaaa atcctcccgg atggccgggg caatgtgttc tgccgcactg gagaaacgta
     5521 tgtctgggat atggaatttg gcgtggacgg gtcactgtac gcggccaccg gcacacgcgg
     5581 catcgtgctg aagatagacc gtgacggcaa aatcgtggac aaggtgcttg acacgagcga
     5641 tcctcacttg atgaccctgg ttcccgacgg acggggcggt ttctacgcgg gcaccgacgg
     5701 gaatggtctc gtgtacgcca tcgaaaacga tcggtcgcgc ctcctgttcg cggcggcgga
     5761 gcgggagatc cacacgctgg ccgtcgggcc tgacggccgg gtgtacgcgg gcgcgacggg
     5821 agcgagaaac cccggcgaac agagcacgga acggtcgccc tccggcgcgg tttaccgtct
     5881 ggagccttct ggagcggcga cgaagctctg gacggctccg taccctcttg tactctccat
     5941 tgtgccgtac gactcctccc gcgtgcttgt cggtgttggc ccccggggcg tgctctatct
     6001 tctcagcacc gacggccgaa cgcagcgcgt ggctgacacc ggcgaatccc agcctgcgtg
     6061 tttcctgaag cgttcgtcgg gagaagttct tgtcggcatg ggcaattccg ggaaaatcaa
     6121 gcgcctgacg acgaaaccag gaacccgtgg cgtgtttgtt acgcctgcgc gaaacgcagg
     6181 gtacgtcgcg caatggggac gcgtggaaat acgcggagtt acccctcccg ggacgagagt
     6241 gcatgtcgaa tcgcgcaccg ggaacaagtc ggacccggcg acggaatgga gcgcctggca
     6301 gccgcttttc ggcgaggaac aggaccggat acagagcccg gcggcacagt acgcgcagct
     6361 ccggttgacg cttacaaggg cagaaaacgc gccctctcct caggtttcca gcatcgggat
     6421 taccagcctg caggtcaacc tgcgtcccga gatttcaatg gtgtcggtcc agccagttcg
     6481 gtccacccag acaggctcgc agagttcctc gcaacagggc gaaagctcaa gcgatcagtc
     6541 gtccaccccg cggacaacta ctccgcgcgg ggcgccgacg aagcgatccc tcgtttcggt
     6601 acgctggaat gtcaccgatc ccaacggcga tgatctggtg tactcgctct atttccgacg
     6661 ggtgagcgaa acgacatggc atctgctgga tgaattgctc acgcggaatt cctacgtgtg
     6721 ggataccgaa ggcgcgcccg agggtatgac cgtgttgaaa gtagtcgcgt cggaccagcc
     6781 gagtaatccg tggcagacgg ctctctccgc agaacgggaa agcgctgcgt ttccggttga
     6841 ctactcgggt cccgtggtaa gcgaactgac tgcctctccc aggccggacg ggtccgtcgt
     6901 gttgaggggt gtggggtcag ataccgtcgg gccgatcaac gacggtgcgt attccgtgaa
     6961 cagcggggaa tggcgtccgt ttttccctgt ggacggcata ttcgacggga aacgggaagc
     7021 gtttgaggtg ctgaccgagg c
//
LOCUS       DAOSOO010000168         7000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10810_length_7000_cov_5.447516, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000168 DAOSOO010000000
VERSION     DAOSOO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10810_length_7000_cov_5.447516"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2611
                     /locus_tag="PLT86_10940"
     CDS             <1..2611
                     /locus_tag="PLT86_10940"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT37167.1"
                     /translation="KNAFHAQDVSEKEPFCIVIPPPNVTGSLHMGHALTVTIEDIMIR
                     WHRMLGHNTLWMPGTDHAGIATQMVVERELKRTEHKTRHDIGREEFVKRVWQWKEQYG
                     NRISLQLRALGSSLDWQRERFTMDAQLSRAVREVFVRLYEEGLVYRAHRLINWCPRCQ
                     TALSDLEVEHDEGHAGSLWHIAYPVTGEPGREVVVATTRPETMLADTAVAVHPEDERY
                     TDLVGKTVTLPLVGRQIPVVADDILVDREFGSGVVKVTPGHDFNDFETGLRHNLPMIS
                     MLDSWAKTTDVCGKYAGVDRFEARKMVLADLEVEGFLRKTESHSLSIGKCQRCETIVE
                     PMLSDQWFVRTKPLAEPAVAAVEDGRVRIIPESHTKTYFHWMRNIQDWCISRQLWWGH
                     QIPAWYCRTCGHVNVSREDVTACAACGSKEVVRDEDVLDTWFSSALWPFSTLGWPEDT
                     PALKTFYPTNVMETGYDILFFWVARMMMMGMKFMGEPPFKTVYLHGMVLDKHGQKMSK
                     VKGNVVDPLDVTQEVGADSLRFYLAVMSGQERSLRFDMERVVGYRNFINKVWNAARFA
                     LNHISELPAEPFSPQTLVLSLADRWILSRLAQTVSRVSSALEEYRFDIAADTLYHFFW
                     HEFCDWYIEMVKTPLADDTHTERKHAAMRVLAHALDVSLRLLHPIMPYVTEELWQKLP
                     VPRHSASMSGVTDTIVLASYPRPDAESRDEQAEREMAAIQETVTAIRTIRSETRVPPA
                     AKIAVVVRADGFVRATLERHASYVTDLARLESLTVSSDASRPRASATAVVQGAAIYVP
                     LEGLIDFEQEKQQLAKEIARIESYVAATRKKLGNESFVSRAPAEVVQTEREKIAAAEA
                     SLERLRDTLVSIG"
     gene            2687..4126
                     /gene="purB"
                     /locus_tag="PLT86_10945"
     CDS             2687..4126
                     /gene="purB"
                     /locus_tag="PLT86_10945"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT37168.1"
                     /translation="MPNETAPRYADPLVSRYASPEMARIFADLTRFRQWRRLWIALAE
                     AEKELGLPISEEQLAEMRAHAEDIDIARADELERKLRHDVMAHVHAFGEQCPGARPII
                     HLGATSCYVTDNSELWQIREGLRLLRARIVRLITNLADFARTWRSQPALGYTHFQPAQ
                     LTTVGKRATLWLHDFVEDLLEIEYRLDRLAFRGLKGTTGTQASFLELFDGDHARVREL
                     EARVARKMGFERVVPVTGQTYSRKQDWQVLQALSGVAQSVHKFGNDIRLLQHLKEIEE
                     PFGSSQIGSSAMAYKRNPMRSERATGLARWVLNVVQNASMTASEQWFERTLDDSANRR
                     LAIPHAFLGADALLILTTNIVKGLVVYPAMIEQHIRQELPFMATENLLMAAVRAGGDR
                     QLLHETIRVHSQAAGDAVKAGGRNDLLERLANDPGFPLESAEIARELEPKRFVGRAPE
                     QVDEYLDEVVAPILAARANLKPPETEVKV"
     gene            4123..5469
                     /locus_tag="PLT86_10950"
     CDS             4123..5469
                     /locus_tag="PLT86_10950"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT37169.1"
                     /translation="MSGETPSNPREDATRIVLAAISAADPKEGVKRSFRIAANELAVG
                     TWHRRTEEIGRIRVVGAGKASAHMAAALEEVLGDRITSGIVITKYGHGVPLRRIKVVE
                     AGHPVPDEQGVEATLELMQHVAGSAPGDVIFVLLSGGASSLLVAPAQGITLADKKTTT
                     SVLLSCGATIEELNCARKHLSRVKGGQLLRACGSAQVVSLILSDVIGDPLDVIASGPT
                     VPDPTTFGDALGVIDRLGIRKSLPPRVVSHLEAGDRGEYPETPKPDEGSFSNGHALLV
                     GTNSIALKSAAYEAERRGYRAHVLTSSLRGEASEAAKVVCALAEGVPAGERPTALILG
                     GETTVTLSRDAGKGGRNQEFALSAAREIRGRSDITVASVGTDGTDGPTDAAGGLVDGA
                     TWERALRRGLSPDAALRRHDAYPLLSATGDLVMTGPTGTNVMDVIVALAVPRKNTF"
     gene            complement(5703..6143)
                     /locus_tag="PLT86_10955"
     CDS             complement(5703..6143)
                     /locus_tag="PLT86_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT37170.1"
                     /translation="MTLVRYFPGFARPVFGRPTDELARWPSDWPFDAPLNAEWYPRAD
                     VVETDNAWEISVELPGIEKDAVKVSVENGVLSISGETKAEHKDEGRGVYRLERRYGSF
                     SRTFELPDGVDAEKIEAVFKNGLLKLTVPKTAEVLPKTIEIKHE"
     gene            complement(6449..>7000)
                     /locus_tag="PLT86_10960"
     CDS             complement(6449..>7000)
                     /locus_tag="PLT86_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37171.1"
                     /translation="RPFADPVVPTSDVGEWDRFQILLTGAPPIAVGDQLFIYYRGTAR
                     RHNKVKGEFEPRIDSDQDRRTMSIGLATLRLDGFASIAASYDGGYITTRPTVLGGDVL
                     SVNAVSDYGEVRLEILDEKGETIPGYSAQDCIPVSVDCVEAEVAFRDRRSLSEISGRT
                     IKLRFHLKNARLYSFRATYSSHG"
BASE COUNT         1520 a         2034 c         2097 g         1349 t
ORIGIN      
        1 aaagaacgcc ttccacgcgc aagacgtctc cgaaaaggaa cctttctgca tcgtaattcc
       61 gccaccgaat gttacgggta gtctccacat ggggcacgcg ctcaccgtga ctattgagga
      121 catcatgatc cggtggcatc gaatgctcgg gcacaatacg ctgtggatgc cgggaactga
      181 tcacgccggc atcgcaacgc agatggtcgt ggagcgggag ctcaaaagga cggaacacaa
      241 aacccggcat gacatagggc gcgaggaatt cgtcaagcgc gtctggcaat ggaaggagca
      301 gtacggaaac cgtataagcc tccagctccg cgcgctcggt tcctcgctgg actggcagcg
      361 cgagcgcttc accatggacg ctcaactctc ccgcgcggtg agggaggtgt tcgtccgctt
      421 gtacgaggaa ggtcttgtct accgcgccca tcgattgatc aactggtgcc cgcgatgcca
      481 gaccgcgctc agcgacctcg aggtcgaaca tgacgaggga catgccggaa gtctctggca
      541 catagcctac cctgttacgg gcgaacccgg tcgcgaagtg gtggtggcca ccacgcgccc
      601 cgaaaccatg ctcgcggaca ctgcagtcgc cgttcatcct gaggacgagc gttacacaga
      661 tctcgtcggc aaaaccgtga cgttgcctct tgtcggcaga caaatacccg tggtcgccga
      721 cgatattctc gtcgacaggg agttcggttc aggggtggtg aaggtcacgc ccgggcacga
      781 tttcaacgat ttcgagacgg gcctgcgcca taacctgccc atgataagca tgctcgactc
      841 ctgggcgaaa acaaccgacg tgtgcgggaa gtacgcagga gtggacaggt tcgaagccag
      901 aaaaatggtg ctcgcagacc tcgaagtaga gggcttcctg agaaaaacgg agagccattc
      961 gctttccatc ggaaaatgcc agcgttgcga aaccatcgtc gagccgatgt tgagcgacca
     1021 gtggttcgtc cggacgaaac cgctcgcaga gcctgccgtt gcggcggttg aagacggtcg
     1081 tgttcgcata attccagaat cacacacgaa gacgtatttc cactggatgc gcaacattca
     1141 ggattggtgc atcagccgcc agctgtggtg ggggcatcag attcccgcgt ggtactgccg
     1201 gacgtgcggt cacgtgaacg tcagccgtga ggacgtgact gcgtgcgccg cgtgcggcag
     1261 taaagaggta gttcgcgatg aggacgtcct cgacacgtgg ttcagttccg cgctgtggcc
     1321 gttctctacc ctcggctggc cggaagacac gcccgctctc aaaacgttct accccaccaa
     1381 cgtcatggaa accggatacg acatcctctt cttctgggta gccagaatga tgatgatggg
     1441 gatgaagttc atgggtgaac caccgttcaa aacggtgtac ctgcacggga tggttctgga
     1501 caaacacggc cagaagatga gcaaggtcaa agggaacgtg gtggatccgc tcgatgtgac
     1561 gcaggaagtg ggcgcggaca gcctgaggtt ctaccttgcg gtgatgtcgg ggcaggagcg
     1621 aagcctgcgc tttgacatgg aacgagtcgt tgggtacagg aatttcatca acaaagtctg
     1681 gaacgccgcg cggttcgcgt tgaatcacat cagcgagctt cctgccgagc ctttctcccc
     1741 gcagactctt gtcctttctc ttgcggatag atggattctc agcagactgg cgcaaaccgt
     1801 ttcgcgcgtt tccagcgcac ttgaggagta tcgattcgac atagctgccg acacgttgta
     1861 ccatttcttc tggcacgagt tctgtgattg gtatatcgag atggtcaaga cgccgcttgc
     1921 tgacgatact cacacggaaa gaaagcatgc agcaatgcga gtactcgcgc acgcgcttga
     1981 tgtaagcctg cgcctgctcc atccaatcat gccgtatgtc accgaggaac tctggcagaa
     2041 actacccgtg ccgcggcaca gtgcttcgat gagcggagta acagacacga ttgtgctggc
     2101 gtcctatccc aggccggatg ccgaatcgcg ggacgaacag gcggaacggg agatggcagc
     2161 gatacaggaa acagtaactg cgatccggac gatcagaagc gaaacacgtg ttccaccggc
     2221 cgcgaagata gcagtggtcg tccgcgcgga cggtttcgtg cgcgcgacgc tggagcgaca
     2281 cgcctcctat gtgacggacc ttgcgcggct ggagtcgctc accgtttcga gcgacgcctc
     2341 acgcccgagg gcgtccgcca cggccgttgt tcagggagca gccatatatg ttccccttga
     2401 gggtcttatc gactttgagc aggagaaaca acagctggca aaggaaattg cacgaatcga
     2461 atcgtacgtg gcggcgacgc gaaagaaact gggaaacgaa agcttcgtga gccgggcccc
     2521 agcggaggtc gttcaaaccg aacgcgagaa aattgcagcg gcggaggcat cactcgagcg
     2581 attgcgggat acgcttgtat cgatagggtg aactgcgccc gcaagtttat gaccttgccg
     2641 ccgaaaacgg aactcagcaa gaactcccag ccgagggcga cgaaaaatgc cgaatgaaac
     2701 cgcaccccgt tacgcggacc cgctcgtttc gcggtacgcc agtccggaaa tggcccgtat
     2761 cttcgccgac ctcacacggt tcagacaatg gcgccgcctg tggatcgctc tcgctgaagc
     2821 ggagaaagag ctggggctcc cgatcagcga agaacaactc gcggagatga gggcgcacgc
     2881 cgaagacata gatatcgcac gtgcggacga actggagcgc aaacttcgtc acgacgttat
     2941 ggcgcacgtg cacgcattcg gcgagcaatg cccgggcgcc cgaccgatca ttcacctggg
     3001 cgccacaagt tgttacgtca cagacaatag cgagctctgg cagattcgtg agggattgag
     3061 acttctccgg gctcggattg tcaggcttat cacgaacctc gcagatttcg cacgtacatg
     3121 gagatctcag cccgcgcttg ggtacaccca tttccagccg gctcagctca ccacggtcgg
     3181 gaaaagggcg acactgtggc tgcacgattt tgtggaggat ctcctcgaaa tcgagtaccg
     3241 actcgacagg ctcgcgtttc gtgggcttaa gggcacaaca ggcacgcagg caagtttcct
     3301 cgagctgttc gacggagacc atgcccgggt gagggaactg gaagcccgtg tggcgaggaa
     3361 gatgggcttc gagcgcgtgg ttccggtcac agggcagacg tactcccgca agcaggactg
     3421 gcaagtcctc caggcgctct ccggcgttgc ccagagcgtc cataagttcg gcaatgatat
     3481 acgcctgctc cagcacctca aggagatcga agagccattc ggctcttcgc agataggttc
     3541 atccgcaatg gcctacaaaa ggaacccgat gcgcagcgag cgggcaaccg gcctcgcccg
     3601 ctgggttctc aacgttgtcc agaatgcgtc aatgactgca tcggagcaat ggttcgagcg
     3661 cacgcttgat gactcagcaa accgccggct ggcgatacct catgcattcc tgggcgccga
     3721 tgccctgctc atactcacga caaacatcgt gaaaggtctt gtcgtctacc cggcgatgat
     3781 tgaacagcac atccgccagg aactgccctt catggcaacg gagaacctcc tcatggctgc
     3841 cgttcgggcg ggcggcgaca ggcaattgct tcacgagaca atacgtgttc attcgcaggc
     3901 tgcgggagac gcggtgaagg ccggaggcag gaacgatctt ctggaacgcc tggcaaacga
     3961 cccgggcttc ccccttgagt ccgcagagat tgcgcgggaa ctggagccga aacgttttgt
     4021 cgggcgcgca cccgaacagg tggatgagta tctggacgag gtggtggcgc caatcctcgc
     4081 ggcacgtgcg aacctgaagc ccccggaaac cgaggtcaag gtgtgagcgg agaaactccc
     4141 tccaatcctc gcgaagacgc gacgcgcatc gtgctggcgg cgatatccgc ggcggacccc
     4201 aaagagggcg tgaaacgctc gtttcggatt gccgcgaacg aactcgctgt cgggacctgg
     4261 caccggcgta cggaggaaat tggccggatc cgtgtcgtcg gtgcgggcaa ggcttctgca
     4321 cacatggcgg cggcattgga ggaggtgctt ggagaccgga tcacgagcgg gattgtgatc
     4381 acgaagtacg gtcacggagt acctctgcgt cgcatcaaag tcgtcgaagc gggacatcct
     4441 gttcctgacg agcagggtgt ggaagcaacg ctggagttga tgcagcacgt cgcaggtagc
     4501 gcaccgggtg acgtaatatt cgtcctcctg tccggcggtg cgtcatctct tctggtggct
     4561 ccggcgcagg ggattaccct cgcggacaag aaaacaacaa cctcggtgtt gctctcgtgt
     4621 ggagccacca ttgaggaact caattgtgcc cgcaagcatc tttctcgtgt caagggagga
     4681 caactcctcc gcgcgtgcgg aagtgcccag gtggtttcgc tgattctctc cgacgtgatc
     4741 ggtgaccctc tcgacgtaat cgcgtccggt ccaaccgttc ccgaccccac gacgttcggc
     4801 gatgcgcttg gggttattga ccgactcggc attcgtaaat cgctgcctcc gcgggttgta
     4861 tcccacctcg aagcgggcga caggggggaa tacccggaaa cgcccaagcc ggacgagggg
     4921 tccttttcga acggccacgc tttgctggta ggaacgaaca gcatcgcgct caagagcgcg
     4981 gcgtacgaag cggaaagacg aggatacagg gcacacgtcc tcacctcgtc gctccgtggc
     5041 gaggcgagtg aggctgcaaa ggtagtgtgc gccctggcag agggggttcc tgccggcgag
     5101 cggccaactg ccctcattct aggaggggaa accaccgtaa cgctttctcg agatgccggg
     5161 aaaggcggga ggaatcagga gtttgccctt tccgccgctc gcgaaatccg cggacgatcc
     5221 gatatcactg tcgcctcggt cgggaccgat ggcacggatg gtccgaccga tgcggccgga
     5281 ggacttgtgg acggtgcgac gtgggaacgg gcactccgtc gcgggctctc acccgatgcc
     5341 gcgttgcggc ggcacgacgc gtacccgctg ctgagtgcga cgggtgacct cgtaatgacg
     5401 ggtcctaccg gaacgaacgt catggatgtg atcgttgcct tggctgttcc ccgcaagaac
     5461 accttctaac cgaccatccg ccccccccca cgcaaaattc cgtcattgca gctcgacggc
     5521 gtttctggtt ctgccgtacg tgattgtgca agaacgcgtt ccggctcagc ccgtacaata
     5581 aatcgcggcg caagaccttc tgaggatatc gcgccgcgat tacttatcta tcatgaaggg
     5641 aaccgaccgg aatccggccg gttcctcgtt ttttcgggtg ggcaccgaag tgcccgaccg
     5701 ctttactcgt gcttgatctc gatcgttttc ggaagcacct cggccgtttt cgggaccgtg
     5761 agcttcagca gaccattttt gaacacagct tcgatctttt ccgcgtctac gccgtcagga
     5821 agctcgaagg tgcgagagaa gcttccgtac ctgcgctcca accgatatac gccgcggcct
     5881 tcgtccttgt gttccgcttt cgtctcaccc gagatcgaaa gcacgccgtt ttcaacagaa
     5941 accttcaccg cgtccttctc aattcccggg agctccactg agatttccca ggcgttgtcg
     6001 gtttccacga catccgcacg cggataccac tcagcgttca gcggagcatc aaacggccaa
     6061 tcactcggcc agcgcgcaag ttcatcagta ggacgcccga acaccggacg ggcgaaaccg
     6121 ggaaaatatc taacgagtgt catggcagtg ccctcctttt tcttccacgt ttgcgttgtg
     6181 cgttcgttct cgcttccgca ggaggagtgt tgcaactgac gtgccagtct ctgctcagcg
     6241 atttcccgca tcgccgcaag atattgaaaa acagtccctt atcattttgg ccaggactga
     6301 gccgggcaat gtgcggcacg cccgaaaagg caaagcgtca cgattgtatg ccaaaacgac
     6361 accgtccaat tctagaatgg cacgaagaat gtcattatgg catcttcacc cacatttgcg
     6421 acgaggcgcg atcattcgac cccatgggtt acccgtgaga tgaataggta gcgcggaagg
     6481 aatacagccg ggcgtttttc agatggaacc ggagttttat ggtccgcccg gagatttccg
     6541 agagggatcg ccgatcccgg aacgcgactt ccgcttccac gcagtcaacc gagacgggaa
     6601 tgcagtcctg cgcggagtac ccgggaatgg tttctccctt ttcgtcaagg atttccaatc
     6661 gaacctcacc gtaatcagaa accgcgttca cggagaggac atccccaccc agaaccgtcg
     6721 ggcgcgtcgt gatgtaaccg ccgtcatagc ttgccgcaat tgacgcaaaa ccgtccaaac
     6781 gaagggtcgc taacccgatt gacatggtgc gacggtcctg atccgaatcg atgcgaggtt
     6841 caaactcgcc cttcaccttg ttgtgccgcc tcgctgttcc ccggtagtaa atgaacagct
     6901 ggtcgcccac cgcgattggc ggagcgccgg taagcaggat ctggaatctg tcccattccc
     6961 cgacatcact ggtcgggact acagggtccg cgaacggacg
//
LOCUS       DAOSOO010000169         6964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_10897_length_6964_cov_5.122594, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000169 DAOSOO010000000
VERSION     DAOSOO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10897_length_6964_cov_5.122594"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLT86_10965"
     CDS             <1..670
                     /locus_tag="PLT86_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOT37172.1"
                     /translation="SMEARMTITNMAIEAGAKSGIIPADNITEAYIRERRHDRPNYRV
                     YHSDPDAKYVAHHTIDVATMEPMVAFPNLPSNAHSIGDVRSDIGIDQAYIGSCTNGRI
                     EDLRIAAAILKGHKVKARTMVVPATTAIWKQANDEGLFDIFYDAECVISTPTCGACLG
                     GHMGVLAADEVCISTTNRNFVGRMGSPKAKVYLASPATVAASAIEGRIVDPRPYLTKT
                     AAAE"
     gene            710..1195
                     /locus_tag="PLT86_10970"
     CDS             710..1195
                     /locus_tag="PLT86_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT37173.1"
                     /translation="MRGRAHVYPRAHINTDEIIPARYLNVHSEAELAKHAMEDIDAEF
                     VKRVQPGDIIVAGDDFGCGSSREHAVWALRGAKVSAVIANNFARIYFRNSINNGFPAI
                     ECQDIAQKVQTGDELEIDLKAGSITNVTRKETYTFVPMADFAVEIMQAGGLLEYVKAK
                     A"
     gene            1226..1927
                     /locus_tag="PLT86_10975"
     CDS             1226..1927
                     /locus_tag="PLT86_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37174.1"
                     /translation="MLRRTTIILGTASILAAAAAGIRIERAWCARSALDTTRAPDDIA
                     ALPPLESTIAGLYEKPPMPGRRVLLTGPILVLDTIRTVRPIATSTDTIPVPEGLIFLA
                     DSLTGHRIVLLRRLDEDGSAWVELTPLPRSVSVPHENPRSTPLAHLAHFWQAGWSDLH
                     GPGGFVWLGLDSAIIRTRDIVNPDSPPYRLARLIRSGSFRVAKAPRVWDMANILLAVA
                     AVACALGAWRSRKKA"
     gene            1924..3270
                     /gene="hisD"
                     /locus_tag="PLT86_10980"
     CDS             1924..3270
                     /gene="hisD"
                     /locus_tag="PLT86_10980"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT37175.1"
                     /translation="MRATERTAKEVPVRIPCIPYRTGDSPERLAAICARGDSASAAIR
                     ARVSTVLEAVRAQGDAAVVEFTEQFDHVRLTPAELRVPMMVCAQALESLEPPLAEALR
                     AAHDNIRRFHEHHRPASWTVDDGDGVVLGKRYAPVAAAGLYVPGGTAPLFSSVLMSVV
                     PAKVAGVPRIALCTPPSEDGMPSQATLAACALAGVDEVYRVGGIQAIAAMAFGTGTIA
                     PVDVIAGPGNAFVQEAKRQLAGTVGIDMVAGPSEIVVIADETANPAWIAADLLSQAEH
                     GSGSEASVAIVTSEDLGERIAREVDSRMRLLPRSAMVEKALSAYGTIFFVPDLGTACE
                     LANRIAPEHVELHTADPWALVDCIRCAGAIFIGAHSPEPVGDYYAGTNHILPTGRAAR
                     FASSVGVDTFLRATSLVAYTGQRLAKTAAHIVTLAEAEGLSAHARAAAIRVEHTSQ"
     gene            3290..3949
                     /locus_tag="PLT86_10985"
     CDS             3290..3949
                     /locus_tag="PLT86_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT37176.1"
                     /translation="MKTAFWMRSAAGIGGTFLFVWLFAGCAAKVADVREEPETTVYAP
                     GADTVVTTPARPDVYATRTVRTRANVRTQPSTSADVVRVLDPGASVGLIAVHNGWFNV
                     MVDSVSGSTGWMWGPLLALTREDRFGAALDMAKPNFGADTLFSAVFYEDNILKVSLDL
                     AWRDLTTAQKQEVVSQIGTAWRAAAQQMGFRPAPEIRFLSNMNYEMARYTSKGQVVVK
                     H"
     gene            3997..4950
                     /locus_tag="PLT86_10990"
     CDS             3997..4950
                     /locus_tag="PLT86_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT37177.1"
                     /translation="MIFIPYDGAYLGKTGDLASFSFAVLGDSHTHRTANPEDPAPELL
                     RIIEELNLLRPAFAVSAGDLIRGYVNETEKAEIEATGDSPTLEKRLRLEHSGAVRAYE
                     SLAMPFYPCIGNHDVREPVSERVWRDIWGGRYYSFDYADCHFVVLDAEISKDTESVAG
                     VQLEWLERDLATYAVGKRLFISLHRPWWFDYPLHTATWKHPGGRNDWNDIVDPILRRY
                     DLQAIFCGHKHRFEFEHREGVPHIVTGGAGGEINDAPEIGGVVHYTWVSVYPGGFTWS
                     VIVPGHILSPERIARAKNNFREVIPWLPPEMGGTRKVRVED"
     gene            complement(5225..6664)
                     /locus_tag="PLT86_10995"
     CDS             complement(5225..6664)
                     /locus_tag="PLT86_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37178.1"
                     /translation="MKTGRHIVVAAFAAVLVLSSLARATDVSGNITTTTWTKANSPYR
                     VKGAIEVVLNNILTIEPGVDVLFDADVKITAYGLIRATGTAADSIRFLKGTAAEWGGI
                     SVTSSGTVTPPNDSSAFAYVRISGAKNDGSPGYGGAIGLSQTNLKLSLDHCVLSGNTA
                     ATEGGAVSVDNGAILYARNCLFANNTSPIGGGISFRANTTYGTISDCVFQGNTSATDG
                     CAIGSRTPHAAFTRCLITGNTHANNTSTVDCFSGSNITMVNCTIANNTTTKVFDSTSG
                     GKAALRNCIVWGTGTVMLEAPLPGTSDPWTVTYSDIKGSWTGTGNINADPLFINAAAG
                     DFTLGTGSPCINTGDPADPTDPDGSRVDMGAYPTSGSTAVLAPATLARFALYANAPNP
                     FNPATTIRFDLPSSGFVTLAVYDINGRLVRNLLVGLAASAARTFPAGQHSVTWDGCDN
                     HGRAVASGVYLVRLTAPEGVLTGRMVLLR"
BASE COUNT         1274 a         2201 c         2203 g         1286 t
ORIGIN      
        1 gagcatggaa gcccgcatga ccatcaccaa catggcgatc gaggcgggcg cgaagtcggg
       61 catcatcccg gcggacaaca tcacggaggc gtatatccgt gagcgccggc acgaccggcc
      121 gaattaccgg gtgtatcact ccgaccccga cgcgaagtac gttgctcacc acacgattga
      181 cgtggcgacc atggaaccga tggtggcgtt tccgaacctg ccgtcgaacg ctcactccat
      241 tggcgacgtt cgctcggata tcgggataga ccaggcgtac atcggctcct gcaccaacgg
      301 gcgcatcgag gatctgcgca tcgccgcggc aattctcaaa gggcacaagg tgaaggcccg
      361 gaccatggtg gttccggcaa ctaccgccat ctggaaacag gcaaacgacg aaggcctttt
      421 cgacatcttc tacgatgccg aatgcgtcat atccacgccc acgtgcgggg cctgtctcgg
      481 ggggcatatg ggcgttctgg cagccgacga ggtgtgcatt tccaccacga accgcaactt
      541 cgttggacgc atgggaagcc cgaaagccaa ggtgtatctc gcaagccctg ccacggtggc
      601 ggcatcggca attgagggaa gaatcgtgga tccgcggccc tacctcacaa aaacggcggc
      661 ggcagaataa gcactgacga ttgcatcgac agatacaagg agtattgcca tgcgtggacg
      721 cgcccatgtc tatccccgcg cgcacatcaa caccgacgaa atcatccccg cgcggtacct
      781 gaacgtccac tccgaagcag agctcgcgaa gcatgcgatg gaggacattg acgccgagtt
      841 cgtgaaaagg gttcaacccg gcgatattat tgtcgcgggg gacgatttcg gctgcgggtc
      901 atcgcgcgag cacgccgtct gggcactgcg cggggcgaaa gtcagcgccg tgatcgcgaa
      961 caacttcgcg cgtatctact tccgcaacag catcaacaac gggtttccgg ccatcgagtg
     1021 ccaggacatc gcgcagaagg tgcagaccgg tgacgaactg gagattgatc tcaaggcggg
     1081 gagtatcacc aacgttacca gaaaagaaac ctacaccttc gtcccgatgg cggatttcgc
     1141 ggtcgagatc atgcaggcgg gcggtctgct ggagtacgta aaggcgaaag cctgaaagag
     1201 ctcgcgctat cgggggagga ttgccgtgct tcgacggacc acgataattc taggtaccgc
     1261 atcgattctt gcggcggcgg cggcaggtat ccgtatcgag cgggcatggt gtgcgcggtc
     1321 ggcgcttgat accacgcgcg cgccggacga tatcgccgcg ttgcctcccc tcgagtcgac
     1381 catcgctggt ttgtatgaaa aacccccgat gcccggccgc cgcgtgctcc ttacgggccc
     1441 gattctggtg ctcgacacga tcagaacggt gcggccgatc gccacatcga ctgatacgat
     1501 cccggttccc gagggtctca tctttctggc ggattctctc accggtcatc ggatcgtcct
     1561 gctccgccgc cttgacgagg acgggtccgc gtgggtggag cttacgccgc ttccgcgatc
     1621 agtgtccgtg ccccatgaaa atccccggtc caccccgctt gctcatctcg cgcatttctg
     1681 gcaggcggga tggtcggacc tgcacgggcc gggcggtttc gtctggctgg gtcttgattc
     1741 ggcaattatc agaacgcgcg acatcgtgaa tccggattct ccgccctacc gcctggctcg
     1801 cctcatccgt tccgggtcgt ttcgcgtggc gaaagcgccg agagtgtggg atatggccaa
     1861 catcctgctt gcggtggcgg ccgttgcgtg cgcgctgggt gcgtggcgct cacggaagaa
     1921 ggcgtgaggg caacggaaag gacagcaaaa gaggttcccg tgcgtattcc ctgcattccg
     1981 taccgaactg gcgattctcc tgagcgcctc gcggccatct gcgcccgtgg agattccgcg
     2041 tctgcggcga tccgggcgcg tgtctccacc gtgctggagg cagtgcgcgc acagggcgat
     2101 gcggcagtag tggagttcac ggagcagttc gaccacgtgc gcctgacgcc tgcggaattg
     2161 cgcgttccca tgatggtctg cgcgcaggcg ctcgaatcac tggagccacc gctcgccgag
     2221 gcgcttcgcg ccgcgcacga caacatccgc cgctttcatg agcaccaccg cccggcgtcg
     2281 tggacggtag acgacggcga cggcgtggtt ctgggcaaac gatatgcgcc ggttgccgcg
     2341 gcggggctgt acgttccggg tggaactgcg ccgctctttt ccagtgttct tatgagtgtg
     2401 gtgcccgcga aagtcgccgg agttccccga attgccctgt gtaccccgcc atccgaagac
     2461 ggaatgcctt cgcaggccac gcttgccgcg tgcgcgctcg ccggggtgga cgaggtctac
     2521 cgcgttggag gaattcaggc gatcgccgca atggcattcg gaaccggcac gatcgccccg
     2581 gtggacgtca tcgccgggcc gggcaacgca ttcgtgcagg aagcgaagcg ccagcttgcg
     2641 ggcactgtcg gaatcgacat ggtggccggg ccgagtgaga tcgtcgtaat cgctgacgaa
     2701 accgcgaacc cggcgtggat tgccgccgat ctcctctccc aggccgagca cggcagcggg
     2761 tccgaggcgt cggttgcgat cgtcacgtcg gaggatctcg gcgagcgaat tgcccgcgag
     2821 gtggactcac gcatgcgtct tcttccccgc tcggccatgg tggaaaaggc actgagcgcc
     2881 tacggaacca tcttttttgt tcccgacctt ggaacggcgt gcgaactggc aaaccgcatc
     2941 gctccggagc acgtggaact ccacacagcc gatccatggg cgcttgtgga ctgcatacgg
     3001 tgcgcgggag ccattttcat cggtgcacac tcgccggaac cggtgggcga ctactacgcc
     3061 ggcacgaacc atattctccc gaccggcaga gcggctcgtt tcgcttccag cgtcggggtt
     3121 gatacgtttc ttcgcgcgac cagtctggtt gcgtataccg ggcagcggct cgcgaaaacc
     3181 gccgcccata ttgtcacgct ggccgaagcg gaagggcttt ctgcccacgc ccgcgcggcc
     3241 gcgattcggg tcgaacacac ttcgcaatga ggacacacga agggacgcca tgaaaactgc
     3301 tttctggatg cgctctgctg caggtatcgg cggaacgttt ctttttgtct ggctgtttgc
     3361 cggatgcgcg gccaaggtgg ctgacgtgcg ggaagagccg gaaactacgg tctacgcgcc
     3421 gggtgccgat accgttgtca cgactcccgc ccgccccgat gtgtacgcca cgcgcaccgt
     3481 gcgcacgcgg gccaacgtcc gcacccagcc ctccaccagc gccgacgtgg tcagagttct
     3541 ggatccgggg gcttcagtgg ggctgatcgc ggttcacaac ggatggttca acgtcatggt
     3601 ggattccgtt tccggctcca ccgggtggat gtggggaccg ttgctcgccc tcacccggga
     3661 ggaccgcttc ggcgcggcgc tggatatggc gaagccgaat ttcggggccg ataccctgtt
     3721 ctccgccgtg ttctacgagg acaatatcct caaggtaagc cttgacctgg cgtggcggga
     3781 ccttaccacc gcgcagaaac aggaggttgt gagccagata ggcaccgcat ggcgcgccgc
     3841 cgcccagcag atgggcttcc gtccggcgcc ggaaatccgc ttcctttcca acatgaacta
     3901 cgaaatggcg cgttacacca gcaagggaca ggtcgtcgtc aagcactgat cgtgtccgac
     3961 gttgctccgc tgtctgctcg caaagaaggg gcgcctgtga ttttcattcc gtacgatgga
     4021 gcatatctcg gcaaaaccgg cgatctggct tcgttcagct tcgccgttct gggtgattca
     4081 cacacacacc gcactgccaa tcccgaggat ccggcgccag agcttctccg catcatcgag
     4141 gagctgaacc tcctccgccc cgcgtttgcc gtctcggcgg gcgacctcat ccgtggctac
     4201 gtgaacgaga cggaaaaggc ggagatcgag gcgaccgggg attcccccac gctggaaaag
     4261 cggctgcggc tggagcactc cggcgccgtg cgggcgtatg aatcactggc aatgccgttc
     4321 tatccgtgca tcggcaacca cgacgtccgc gaaccggtca gcgagcgcgt ctggcgggat
     4381 atctggggcg ggcgctacta ctccttcgac tacgccgact gccactttgt ggtgctggac
     4441 gccgagattt caaaagacac cgagagtgtc gccggcgtgc agctcgaatg gctcgagcgc
     4501 gaccttgcca cgtacgccgt cggcaagcgc ctcttcatca gcctccaccg gccctggtgg
     4561 tttgattacc cgctccatac cgccacgtgg aaacacccgg gcggccggaa cgactggaac
     4621 gacatcgtcg acccgatcct ccgccggtac gaccttcagg ccatcttctg cggccacaag
     4681 caccgattcg agttcgagca cagggagggc gtgccgcaca tcgtcacagg cggcgcaggg
     4741 ggagagatca acgacgcacc ggaaatcggc ggcgtggtgc actacacctg ggtgagcgtc
     4801 taccccggcg ggttcacatg gtcggtgatt gtgccgggcc acattttgag ccccgaacgc
     4861 attgcccggg caaagaacaa cttccgggag gtcatcccgt ggctgccgcc ggagatgggt
     4921 gggacccgca aagtccgcgt ggaggattga tcacaccccg ccgcgccacg gacgggtagg
     4981 gcggtctgcc tgcaccaacg cgtggtggct gagcttgtcg aagccccgcg cggcgtttcg
     5041 acaggcttcc tgcgcgtcgc gcaggctcaa tgaccgcgca agacgccgag accgccacgg
     5101 ccccgtgcca cataggtcct attgggtcct gtaggacgca acgaaacggc gtatttctgc
     5161 cattgtaggg gcaattcatg atgcgtgcgc cctgcgcacg acgcccctac ggtgcgaatg
     5221 aaccctaccg caacagcacc atcctccccg tcagcacccc ctccggcgca gtcagccgca
     5281 ccagatacac cccgctggcg accgcgcggc cgtggttgtc gcagccatcc cacgtgactg
     5341 aatgctggcc ggccgggaat gtccgcgccg ccgaggcggc gaggcccacc agaagattgc
     5401 gcacgaggcg cccgttgatg tcgtagacgg caagcgtaac gaagccgctg gacggcagat
     5461 cgaaccgtat cgtcgtggcc gggttgaacg gattcggcgc attggcgtag agcgcgaacc
     5521 gggctagtgt ggccggcgcc agaacggcgg tggaaccgga ggtcggatac gcgcccatgt
     5581 ccacgcgcga gccgtccggg tcggtcgggt ccgccggatc gcccgtattg atgcacggcg
     5641 acccggtccc gagcgtgaaa tcccccgcag cggcgtttat gaacagcgga tcggcgttga
     5701 tgttccctgt gccggtccaa ctgcccttga tatcagagta tgtcactgtc catgggtcgg
     5761 acgtgccggg cagcggggcc tcgagcatca ccgttccggt gccccagacg atgcagttcc
     5821 gcagcgccgc tttcccccct gaggtgctgt caaacacctt ggttgtcgtg ttgttcgcga
     5881 tcgtgcagtt caccatcgtg atgttcgaac cgctgaagca gtccaccgtg gaggtgttgt
     5941 tcgcgtgagt gttcccggtg atcaagcacc gggtgaacgc ggcgtgcggc gtccggctgc
     6001 cgatggcgca tccgtcggtg gccgaggtat tcccctgaaa cacgcagtcg ctgatggtgc
     6061 cgtatgtcgt gtttgcccgg aagctgatgc cgccgccaat gggtgaagtg ttgttggcga
     6121 agaggcagtt ccgggcgtac aggatcgcgc cgttatccac gctgacagcc ccgccttccg
     6181 tggcggcggt gttccccgaa agcacgcagt ggtcgagcga gagcttgagg tttgtctggg
     6241 agaggccgat cgcgccgccg tacccggggc tgccgtcgtt cttcgcgccg ctgatcctca
     6301 cgtacgcaaa cgcggagctg tcatttggcg gggtcaccgt gccggaggac gtcacggaaa
     6361 tcccgcccca ctccgccgca gtgcccttca ggaagcggat gctgtccgcc gccgtgcctg
     6421 tcgcccggat caggccgtac gccgtgatct ttacgtcggc atcgaacagg acatccaccc
     6481 ccggctcgat ggtcaggatg ttgttcagaa cgacctcgat ggcccctttt acccggtacg
     6541 ggctgttcgc cttcgtccag gtggtggtgg tgatgttgcc ggatacgtcg gttgcccgcg
     6601 cgagcgaact cagaaccagc actgccgcga acgcggccac aacgatgtgc cgcccggttt
     6661 tcatggtgtc ccccgtggcg atctgtctga taccaatcag ccgaacaatc agtctgccga
     6721 ttgtctggcc gcgaaaaagg cagtatcacg gcaaacaata ctcaccgttc gtgggtaacg
     6781 caagtactac ctttcgaaac gttttctcga aacgaaaggt aacacatgaa tgacgttttg
     6841 ctccgcaaga agagcagtct ccgcgtccac cccttccacc cccatgaata gaccctgcag
     6901 cgcgcgggcg ggcctttccg aggtgagccc gttttcaagc cacatgccgg gtacgaacca
     6961 ctgc
//
LOCUS       DAOSOO010000170         6908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11024_length_6908_cov_5.546622, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000170 DAOSOO010000000
VERSION     DAOSOO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11024_length_6908_cov_5.546622"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLT86_11000"
     CDS             <1..1115
                     /locus_tag="PLT86_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37179.1"
                     /translation="AQLTSVRRTEILGSLAMSRIRGTADTGNPVTRSELSATGTALNS
                     LVVSAPYPVYRGGFTFALGYARPSDYGQRMNRTGIVTLQGQVFRQSDILRQEGGLSHY
                     AMGFGVEVAPTTSMGLSLRWQHGSVDVRRDITLVAQGLALPDSLVGRYRQHNSIDGLG
                     VVLGTHTVLPAGFQMGLSVSTPTRYTLDGTWGDEYHEAIGANVYHYDYLENKLKYKIR
                     SPWEFGAGLTWTTYALTLAADVWYADWQQARFDGSPYGASSGIDPDTFFETRYRGSLR
                     WHIGGEFLVPVVNTYVRAGYWTGTEPFRGPLLDSGEALTVAGPSKAYSFGAGWLIDRV
                     FALDAAVVFRTGRMGTAQSVEKTGDRRFSVSAAFRM"
     gene            1248..2447
                     /locus_tag="PLT86_11005"
     CDS             1248..2447
                     /locus_tag="PLT86_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT37180.1"
                     /translation="MGRNTAYLEAAREIIPWGTQTNAKRHHAEMGEAMPAFIERANGC
                     RMWDLEGREYIDYRCALGPIILGYRYPAVDEAVRIQMSKGTLFSMASPVELEVAKEIV
                     ELVPCAEMVRFMKTGADANMCNVRIARAYTGRDHIIICGYHGYHDYFMVTQQGNGVPQ
                     AVRDLVTPIGYGDFEKLRETFRTRGETIAAMVMEPFNAKDPAGEFTKEARKLTSEYGA
                     LLIFDEIITGFRLAKGGAQEYLGITPDLASLGKAVANGYPLSVYCGKRQFMSKLDDFV
                     LTTTYAGDTLSLAAARATLAVMQNEPVHKHLYRVGERLQKGIGEVAGKVGVEMAITGN
                     PVQPGFAIVDGDKEKESALNTEWRRQLFAHGVFPHWFVNYSHQPSDIDETIDKAEKAL
                     RATLKRG"
     gene            2471..3727
                     /locus_tag="PLT86_11010"
     CDS             2471..3727
                     /locus_tag="PLT86_11010"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT37181.1"
                     /translation="MDLGAYINELGTKARRAAIEMARVDSATKRDALLRIAEAVEKHS
                     GRILESNARDLAAGKAAGLTQAMLDRLTLTEKRIVAMAHAIAEIADQPDPVGEIEHSV
                     IRPSGIRVGRMRVPIGVIGIIYEARPNVTTDAAALCLKAGNACVLRGGKEALHSNVAL
                     ARVVSETAAAAGLPPDAVQLVSVTDRETVSLMAQAEGLIDVIIPRGGEALIRSVVAAA
                     KVPVIKHYKGVCHTFVDADADLDMARRICFNAKVQRPGVCNAMETLLVHEKVAEAFLP
                     TLFAEYREAGVSLRGCNRTIEIDPTVARATEEDWYAEYLDLILAVRVVSSLDEAIEHI
                     RRYGSAHTDAIVTRSIQTAERFVREVDSSSVMVNVSTRFSDGGEYGLGAEIGISTDKL
                     HARGPMGAADLTTTKWVVTGNGAIRQ"
     gene            3724..4104
                     /locus_tag="PLT86_11015"
     CDS             3724..4104
                     /locus_tag="PLT86_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT37182.1"
                     /translation="MTEAAGDQNPLRTWKEQQRRRRSFARRWKILTLAAAVVVVVAAL
                     FWGGGDGFFVQWEVSREIAALEKENALIRVQNAQLRDHIRRLDSDMSYIERIARERYG
                     MALPHERVYRVIIPPAPTAGSAGP"
     gene            4101..4784
                     /locus_tag="PLT86_11020"
     CDS             4101..4784
                     /locus_tag="PLT86_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOT37183.1"
                     /translation="MMPNDGPSTGWRHWAKANFLTGLVALAPVVITGYVLWYVFTTVD
                     AWLSAALVGIPWLTWRGKPLPGVGFVTVLLIILLTGVVARNVAGFQFLRFWDRQLNRI
                     PMVRGIYRAVKQISQAFFGGTRSVFQAVVLVPFPTRGIYAVGFLTSDAPREVCNKTGQ
                     DLVSVFLPTSPNPTSGYLIMVPRAEVTRVEMTVEEAMKLVISGGTVLPPEAESGADGV
                     TERAQSNAG"
     gene            4802..5581
                     /gene="recO"
                     /locus_tag="PLT86_11025"
     CDS             4802..5581
                     /gene="recO"
                     /locus_tag="PLT86_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT37184.1"
                     /translation="MPGVDFTRALVISARPWSESSRIVTILSPDLGKFRAVCRGVQKA
                     QGGMAAALEPITLSEIVLRQSGRGGMAHISAADAVEYFACTKKSLIGLAAAGGLLELC
                     EAAMPEDEPGDAVFDHLLTLLDELEVLLRKREHSERNAINLLWRGILVLSGDFGYGMQ
                     FENCVACGAVRPRAKGFCLAEGGPVCDACSVPNTLNWGPGTEETLRWLAVAEDSALPE
                     RTIPRTQNREIRTLFERYFRFHVPGFDHLRSLDMLVTERDQ"
     gene            5645..>6908
                     /locus_tag="PLT86_11030"
     CDS             5645..>6908
                     /locus_tag="PLT86_11030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT37185.1"
                     /translation="MAVDTMEQIVSLCKRKGFIFQSSEIYGGLNGCWDYGPLGVELKR
                     NVQDAWWRAMTVEEDDIEGLDASILMHPRVWEASGHVAEFTDPLVDCTHCKARFRDDQ
                     IEKEGQCPACGMKGTLTAPRKFNLMLKTFIGPVEEAASVVYLRPETCQGIYVDYQNIM
                     TAMRRRIPFGIAQIGKAFRNEINPRYFVFRSREFEQMEMQYFVKPGMDMDVFNAWRDK
                     RLRYYAELGIRPEKLRLVPHEKLAHYASAAVDIEYEFPFGWGEIEGIHNRTDFDLRRH
                     SEFSGKDLSFFDDQTRERYIPFIIEASSGVNRTLLAYLCDAYDVEQLENDTRTVLHLH
                     PRLAPVKAGIFPLVKRDGMPEKARQIERQLNPYFRVFYDESGAVGRRYRRMDEVGTPF
                     CITVDSQTMQDDTVTLRDRDTMLQERYPV"
BASE COUNT         1477 a         1868 c         2177 g         1386 t
ORIGIN      
        1 ccgcgcaact gacctcagtg cggcgtacgg aaatacttgg ttctctggca atgagccgta
       61 ttcgtggtac ggcggatacg ggcaatcccg taacgaggag cgagctgtcg gcgacaggca
      121 ccgcgctgaa ttcgctggtc gtaagcgctc cgtacccggt gtaccgcggc ggcttcacgt
      181 tcgcgctcgg atatgcgagg ccgtcggact acggccagcg aatgaaccgc acggggatag
      241 ttacgctgca ggggcaggta ttccggcaat ccgacattct gcgacaggaa ggagggctgt
      301 cccattatgc catgggcttc ggcgtggaag tcgccccgac aacgtcaatg ggtttgagcc
      361 tgcgctggca gcacggatcg gtggatgtcc gtcgcgatat cacactggtg gctcagggac
      421 tggccctgcc agactcgctc gtagggcggt accgccagca caattcgatt gacggtctgg
      481 gggtcgtctt gggaacccac acggtactcc cggcgggttt ccagatgggg ctgagtgtgt
      541 cgacgccaac gcgttatact ctggacggta cgtggggcga cgaatatcac gaggcgattg
      601 gggcgaatgt ataccactac gactatctcg agaacaagct gaagtacaaa atcagaagcc
      661 cctgggaatt cggagcagga ctgacgtgga ccacctatgc gttgaccctt gccgccgacg
      721 tctggtacgc ggactggcaa caggcccgct ttgacggaag cccgtatggt gcgtcttcgg
      781 ggattgaccc tgatacgttt tttgaaacgc gttaccgagg ctcgttgcgg tggcacattg
      841 gtggcgagtt cctcgtccca gtcgtgaaca cgtatgtgcg cgcggggtac tggacaggca
      901 cggaaccgtt ccggggcccg ctgcttgatt cgggggaggc gctgaccgta gcgggccctt
      961 caaaggctta ctcgttcggc gcggggtggc tgattgatcg agtgttcgcg cttgacgcgg
     1021 ccgtagtgtt tcggaccggt cgcatgggaa ccgctcaatc ggtagaaaaa accggagacc
     1081 ggagattctc agtttcggcc gcgttccgca tgtgatccct gcgggcgacg ggcgacgcac
     1141 ggttttccgc aaagagcttc ttcagggttg ctcaaaaacc ttcgttgcgt gttcatttta
     1201 tgggagatct ggttcgtttc cgggtaccat ttgagggagg ttgaacaatg gggcgcaata
     1261 ccgcttacct ggaagccgcg cgcgaaatca ttccctgggg cacgcagaca aacgcgaaac
     1321 gtcatcacgc cgaaatgggc gaagcgatgc cggctttcat cgaacgtgcc aacgggtgcc
     1381 ggatgtggga tcttgaggga cgcgagtaca tagactaccg ctgtgccctc ggaccgatca
     1441 ttctggggta ccggtacccg gcagtagacg aagcggtacg catccagatg tccaagggca
     1501 cacttttcag catggcgtcg ccggtggaac tcgaagtggc gaaggaaata gtcgaactgg
     1561 ttccctgcgc ggagatggtt cgcttcatga aaaccggtgc tgacgcaaac atgtgcaatg
     1621 tgagaatagc ccgcgcgtac accggtcgag atcacataat aatctgcggg taccacgggt
     1681 accacgacta tttcatggtc actcaacagg gaaatggagt tccacaggcc gtccgtgacc
     1741 ttgtgactcc aatcggttac ggtgactttg agaaactccg cgaaaccttc cgaaccaggg
     1801 gcgaaacgat tgccgccatg gtgatggaac ctttcaacgc gaaggacccg gcgggcgagt
     1861 tcacgaaaga agcccgaaaa ctgacatccg agtacggggc gctgttgatt tttgacgaga
     1921 taatcaccgg gttccgcctc gcgaagggcg gagcgcagga atatctggga atcacaccgg
     1981 acctcgcctc tcttgggaag gctgttgcaa acggatatcc gctgtctgtg tactgcggaa
     2041 aacgccagtt catgtcgaaa ctcgatgatt tcgttctcac gacgacctac gcaggcgata
     2101 cgctttccct ggcggcggcc cgggctacgc tcgcagtgat gcagaacgaa ccagtgcaca
     2161 aacacctcta tcgcgtggga gagaggctcc agaaagggat tggggaggta gccggaaagg
     2221 tcggcgtcga aatggcgatc acggggaacc ctgtccaacc cgggtttgcc atcgtggacg
     2281 gagacaagga aaaggaatcc gccctgaaca cggagtggag gcggcagctt tttgcccacg
     2341 gcgtatttcc ccactggttt gtgaactaca gtcatcaacc gtccgacatt gatgaaacga
     2401 ttgacaaagc cgaaaaggct ctccgggcaa ctttgaaacg tgggtagggc actgaacatt
     2461 ggggcacatg atggaccttg gtgcgtacat aaacgaactc ggaacgaaag cgcgcagagc
     2521 cgcaattgaa atggcgcgcg tggattccgc gaccaaacga gatgcacttc tgcgcatcgc
     2581 ggaagcggtg gagaagcatt ccgggcgaat cctcgaatcg aatgcccgtg atctggcggc
     2641 gggcaaagcc gccggtctga cccaggccat gctggaccgc cttacgttga ccgagaagcg
     2701 tatcgtagca atggcgcacg cgatcgccga aattgccgat caacccgatc ccgtgggcga
     2761 gatagagcat agtgtcatca gaccgagcgg cattcgcgtc gggaggatgc gcgtgccaat
     2821 cggggtaatc ggtatcatct acgaagcacg tcccaacgtg acgaccgacg ccgcagcgct
     2881 ttgtctgaag gccggcaacg cctgcgtgtt gcgcggtggc aaggaagctc tccattccaa
     2941 tgtggctctg gccagggtcg tatccgagac tgccgcggcc gcgggtctgc ctccggacgc
     3001 cgtgcagctt gttagcgtta ccgatagaga aaccgtgagc ttgatggcgc aggccgaagg
     3061 gctgattgac gtcatcattc cccgcggggg cgaggctctg atccgttcgg tggttgctgc
     3121 ggcgaaggtt ccggtgatca agcattacaa gggggtgtgt cacacgttcg tggatgccga
     3181 cgcagacctg gacatggcgc ggaggatctg tttcaacgcg aaagtccagc gcccgggcgt
     3241 atgcaacgct atggaaaccc tcctcgtgca cgaaaaagtc gccgaagctt ttcttccgac
     3301 gcttttcgca gagtatcgtg aggccggagt ttcactgcgc gggtgcaaca gaaccatcga
     3361 aatcgatccc actgttgcca gagcgacgga agaagactgg tatgccgagt acctcgacct
     3421 catccttgcc gtccgggtgg taagcagcct cgacgaggca atcgagcaca tccggaggta
     3481 cggttccgcg cacacggacg cgattgtgac acgcagtatc cagaccgccg aacgttttgt
     3541 gcgcgaggtg gactcgtcaa gtgtcatggt caacgtttcc acgcgtttct ccgatggtgg
     3601 cgaatacgga ctgggagcgg aaatcggcat atctacagac aaactgcacg cccgcggccc
     3661 gatgggagcc gcggatttga ccacgacgaa atgggtcgta accggtaacg gagccattcg
     3721 gcagtgactg aagcggcggg cgaccagaac cctcttcgta cctggaaaga gcaacagcgt
     3781 cggagacgtt cattcgcacg caggtggaag attctcaccc ttgcagcagc cgttgttgtg
     3841 gtggtggcag cgctgttctg gggcggaggt gatggttttt ttgttcaatg ggaagtgtca
     3901 agggaaattg cggctctgga aaaggaaaac gcgttgatac gtgttcagaa cgctcaattg
     3961 cgggatcaca tccgtcgcct cgacagcgac atgtcataca tagagcggat cgcacgcgaa
     4021 cggtacggaa tggcacttcc gcatgagcgt gtgtacaggg ttattatccc tccagcccca
     4081 acggccggaa gcgcgggtcc atgatgccga acgacggacc ctcgaccggg tggcgacact
     4141 gggcgaaagc caatttcctt accggattgg tcgcactggc gccggtagtg attaccggat
     4201 acgtcctctg gtatgtcttc accacggttg atgcatggct ctcggccgcg cttgtcggta
     4261 ttccatggct tacgtggcga ggaaagccgc tccccggggt ggggttcgtc accgtactgc
     4321 tgatcatact gctgaccggc gtggtcgcga ggaacgtggc gggatttcag ttcctccgtt
     4381 tctgggatcg ccagctcaat agaatcccga tggtgcgagg catctaccgc gcggtcaagc
     4441 agatcagcca ggcatttttc ggtgggaccc gcagcgtgtt tcaggcggtc gtactcgtgc
     4501 catttcccac acgcggcatc tacgcggtgg gcttcctgac ctctgatgcg ccgagagaag
     4561 tgtgtaacaa aacgggtcaa gaccttgtga gcgtttttct ccccacgtcg ccgaacccta
     4621 cgtcgggcta cctgataatg gttccgcgcg ccgaggttac acgcgttgaa atgaccgtgg
     4681 aagaagccat gaagctggtg atttccggcg gaacagtgct gccaccggaa gcggagtccg
     4741 gagcggatgg ggtaaccgag cgcgcgcagt cgaatgcggg ttgaggcggg agagactgaa
     4801 aatgcccggc gttgacttca cgagggcgtt ggtgatctcc gcacggccct ggtcagaatc
     4861 cagtcgcatc gtaaccattc tttctccgga tctgggcaag ttccgtgcag tctgccgcgg
     4921 cgttcagaag gctcagggtg gaatggcggc ggcgctcgaa ccgatcacac tgagcgagat
     4981 agtcctgcgg cagagtggcc gcggtggtat ggcccatatt tccgctgcgg atgcggtgga
     5041 gtacttcgcc tgcacaaaga aatccctgat cggccttgca gcggccggtg ggttgctcga
     5101 gttgtgtgaa gcggcgatgc cggaggacga gccgggcgat gccgtgttcg accacctgct
     5161 caccctgctc gacgagttgg aagttctgct tcggaagcgc gaacacagcg agcggaatgc
     5221 gatcaatttg ctttggcgcg gaatcctcgt gctttctggc gacttcggat acggtatgca
     5281 gtttgaaaat tgcgtggcgt gcggcgccgt tcggcctcgt gcaaaggggt tctgtctggc
     5341 cgaaggaggt cccgtgtgcg acgcatgctc agttccgaat accttgaact ggggaccggg
     5401 aacggaggaa accctccgct ggcttgcagt tgccgaggac agcgcattgc cggaacgcac
     5461 cattccgcgg acgcaaaaca gagaaattcg caccctcttt gaacggtatt tccggtttca
     5521 cgtcccggga tttgaccatt tgcggtcact tgatatgctg gttactgaac gcgaccaata
     5581 ggccgattca gccgacgtta ttgcgaacgg gtcgtcaacg acacaggtca acgaaggagt
     5641 tttcatggct gtggacacga tggagcaaat cgtctcactg tgcaagagga aggggttcat
     5701 ttttcaatcc agcgagatat acggtggact gaacggctgc tgggactatg gtccccttgg
     5761 cgtcgagttg aagcggaacg ttcaggatgc atggtggcgc gccatgacag tggaagagga
     5821 cgatatcgaa ggtctcgacg ccagcattct catgcaccct cgcgtctggg aagcttcagg
     5881 gcacgttgcg gagttcaccg accctctggt ggactgcact cactgcaagg cgcgcttccg
     5941 ggatgaccag atcgagaaag aaggccagtg cccggcctgc ggcatgaaag ggacgctgac
     6001 ggctccgcgc aaattcaacc tgatgctgaa aacgttcatc ggacccgttg aagaagcggc
     6061 gtccgtcgtg tatctgcgcc cggaaacgtg tcaggggatc tacgttgact accagaacat
     6121 catgacggcg atgagaaggc ggataccgtt cgggatcgct cagatcggga aggcgttcag
     6181 aaacgagatt aacccgcgct acttcgtgtt tcggtcccgc gagttcgagc agatggagat
     6241 gcagtacttt gtgaagccgg gtatggacat ggacgtgttc aatgcgtgga gagacaaacg
     6301 actccgttac tacgcggaac tgggcatacg accggaaaaa ctgcgcctcg tgccgcatga
     6361 aaaactggcg cattacgcct ccgctgccgt ggacatcgag tatgagttcc ccttcggatg
     6421 gggtgagata gaaggtatcc acaaccgaac ggactttgat ctgcggcgtc attccgagtt
     6481 ctccggcaag gacctgagct ttttcgacga ccagacgcgc gagcgatata tcccattcat
     6541 catcgaggca tcttcaggcg taaaccgcac actgctcgcg tacctgtgcg acgcatacga
     6601 cgtcgagcag ttggaaaacg acacacggac ggtgctccac ctgcatccgc gtctcgctcc
     6661 ggtaaaagcc ggcattttcc cgctggtcaa gcgcgacgga atgccggaaa aggcgcgtca
     6721 gatcgaacgg caattgaacc cctatttccg cgtgttctac gacgaaagcg gagccgtcgg
     6781 gcgcagatat cgccggatgg acgaggtggg cacgccgttc tgcatcactg tcgattcaca
     6841 aacgatgcag gacgataccg tcacgttgcg tgatcgcgac acgatgcttc aggagcgata
     6901 cccggtcg
//
LOCUS       DAOSOO010000171         6825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11202_length_6825_cov_5.464106, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000171 DAOSOO010000000
VERSION     DAOSOO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11202_length_6825_cov_5.464106"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLT86_11035"
     CDS             complement(<1..785)
                     /locus_tag="PLT86_11035"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT37186.1"
                     /translation="MPAPSSSVHYDAHSFIINGKREFLTSGSIHYFRVPRALWRDRLE
                     KAKAAGLNTIQTYVAWNWHEPREGVFDFAGDHDFEHFLQLCEEFGLFVVARPGPYICA
                     EWDFGGFPAWLLTKRGLTLRHHDPVYLQYVDRWFEELIPIIARHQVSRGGCVILVQIE
                     NELGNVVPEKKDADAYMSHLRDLVRNLGIEVPLITCAGTAEGAIEAVNSHEPADQFPE
                     FRSRQPNMPIHCTEFWTAWYLTWHRDRRWVNRSPEHLAYHTWRV"
     gene            887..1633
                     /locus_tag="PLT86_11040"
     CDS             887..1633
                     /locus_tag="PLT86_11040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37187.1"
                     /translation="MYDELSALYDLFVDWPSRLRNEMPLLEKLGVGVPGQRIVDVACG
                     TGHHALAWAEKGCTVRAFDASREMVERARMRDRAGLVSWGAAGFAEIPKDGWADALVC
                     LGTSLPHAESEAAHRAALAAFARALKPGGVLVVHSRNLARTAGGGETERFLPPLPRKR
                     DGMNVLFWRFYDVLSPSLLDFHLVILEETPDKWTHRVLTSRLCVVPAEQLAAMAEEAD
                     FHELRVLGSLDGRPYDRGNSPDLVLTAKRR"
     gene            1731..2816
                     /locus_tag="PLT86_11045"
     CDS             1731..2816
                     /locus_tag="PLT86_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37188.1"
                     /translation="MPKGQRWNAEWSTEADRTTGFEVRRLTNNPPSSNRHFYFHQISW
                     TPDSSHVIFVSDRGEGSQLCSISMANGEITQLTQDGMAHDATMGLHAPVAYYYTGNEF
                     RAVNVLTLEERTVGVLPEKMKCYSGPSENADGTLLVFAAQIEGGRCMAKTVIASGVTS
                     VILETSTVLSHVNCSLTDPEWIMHCDSTRGDDKPKQRVWVLSTDGMRHWHPYTQTPQE
                     WLTHESWLGMTGKLLICYWPTGIMEINVDGNGARLIAAVNAWHAAASRDGRYCVVDTN
                     WPDRGIHLIERETGRMCKVCESDSGAGDSRAGSEVHPHPSFSPDGKYILFGSQKTGKP
                     EVYVVGTEQVIARQDLWWKPEYHWHRW"
     gene            2783..3772
                     /locus_tag="PLT86_11050"
     CDS             2783..3772
                     /locus_tag="PLT86_11050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT37189.1"
                     /translation="MEAGVPLAPMVAEGRVTIEPLSEQFSRAAGALIRTVLQEEFGAA
                     IRACEDADLEDVLSAYAGPESAAWVAHRDGVVCGVAAVRETESGALELKRFYVRAECR
                     GKGIGGTLLREVERRAAEDGYQQIVLTTTHLMARAQEVYRHFGYQLDSTERKGAVTLH
                     TYRKRLVRAALGARRLSGPLPNTGTGEIAVVVERPRGSCEGWHWDRQTHTLVLTEYYD
                     RPVPVNYGLAPEWVNPADQDALDVILLDDRRMQPGETLVAVPAGVLLRPDGDDKLLAV
                     PAGGAVRPVGLDERSRARVSAWWDDAHQPVGWEGEEGIDRLFARCRRQGAMNG"
     gene            3765..3962
                     /locus_tag="PLT86_11055"
     CDS             3765..3962
                     /locus_tag="PLT86_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37190.1"
                     /translation="MGKRIPKPEWFVCETCGKQTRALRRDVLDEHYNALGKTPLWNCE
                     ECYQQKRRERLSQPGSPDPSS"
     gene            3899..4498
                     /locus_tag="PLT86_11060"
     CDS             3899..4498
                     /locus_tag="PLT86_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT37191.1"
                     /translation="MLPTEEARAPQPARLTRPVVLIGFMGAGKTKVGKIAAQYIGCDF
                     VDTDHIVERSAGKTIPQLFAEIGEERFRHLETEAIEHALNTHGGIIALGGGAATREEN
                     WQLLRRSGAMTIYLQASPRTILARVVDKGTRPLLAGLDRQQMLEKITSLLEQREPWYL
                     RADITLPTDNSLDKYGMADSLVARLKEAGISAAESHRTS"
     gene            4495..5322
                     /locus_tag="PLT86_11065"
     CDS             4495..5322
                     /locus_tag="PLT86_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37192.1"
                     /translation="MNGRQKTTLWREHVALWRDAWRRVIAHRRQVVLITLLLWLPWRF
                     VVPLVDHRLGFSAFAGFPLEWIADALYDPLYGATILAVLAEMYPISVRSALLRGFWLV
                     PRLWLIEIKVAIWGLAVPYLILHLGAAIVLTLWPSYGAARALFAVTFVSMAWVLVVVV
                     RYMLAAAVMVAETQGHTWNEEQAPSTGWALFAARQLIRGNVRQAVLVMAVGQGVISLV
                     VSLLPSGDTVPAQFLNAIGMVVTALWWALLWSFGQFCLSRARGVTAQEATYGRQKAR"
     gene            5300..5665
                     /locus_tag="PLT86_11070"
     CDS             5300..5665
                     /locus_tag="PLT86_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT37193.1"
                     /translation="MDVRKLVDHLDRRRMVLCHNGTIYSQALFGELTPDEVCPGVFAY
                     APGWGVLDPGMTMEAHEHPIAEFYVFVSGKGRMRLGDTWFDVEPNMSVNIPPGVEHEL
                     ANDATSPLPLVFVSVGLRL"
     gene            5792..6211
                     /locus_tag="PLT86_11075"
     CDS             5792..6211
                     /locus_tag="PLT86_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOT37194.1"
                     /translation="MPRPRNPRQVRCIPCTDYFKPRGIPLRALEEVTLAVEELEALRL
                     ADLEGMEQVAGAEQMEVSRPTFARVLASARQKVADALVNGKALRIEGGVFRMAENVPL
                     CGRHGHGWGQGRGGRCGRRFRGGQGFDGGEAPSEQSA"
     gene            6643..>6825
                     /locus_tag="PLT86_11080"
     CDS             6643..>6825
                     /locus_tag="PLT86_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT37195.1"
                     /translation="MKIAVPVETNEGLKSIVAGHFGHAPYYAFVEGKTRHCVIVPNAS
                     SHNGGDKLPPEWLFDEG"
BASE COUNT         1297 a         1880 c         2354 g         1294 t
ORIGIN      
        1 acccgccagg tgtggtaggc caggtgttcc ggagagcggt ttacccagcg gcggtcgcga
       61 tgccacgtga gataccatgc cgtccagaac tcggtgcagt ggatgggcat gttcggttgc
      121 cgggaacgga attcggggaa ctggtctgca ggttcatggc tgttgaccgc ctcgatcgcg
      181 ccttccgcgg ttccggcaca tgttatgagc gggacttcga tgccgaggtt gcgcacgaga
      241 tcgcgcaggt gggacatgta ggcatcagca tctttctttt ccggtacgac gttccccagt
      301 tcgttctcga tctggacgag gatgacgcaa ccgccgcggg agacctgatg gcgcgcgatt
      361 atgggaatca gttcttcgaa ccagcggtcg acgtactgga ggtagacggg gtcgtggtgc
      421 ctcagggtga gaccgcgttt tgtcagcaac cacgcaggaa atccgccgaa atcccattcc
      481 gcgcagatgt acgggccggg gcgcgcgacc acaaacaggc cgaactcctc gcagagctga
      541 aggaagtgct caaaatcatg atcacctgcg aaatcgaaca ccccttcccg cggttcgtgc
      601 caattccacg cgacgtaggt ctggatggtg ttgaggccgg cggctttcgc cttttccagc
      661 ctatcccgcc agagggcgcg cggaacgcgg aaatagtgaa tggaaccgga ggtgagaaac
      721 tcacgttttc cgttgatgat gaagctgtgg gcgtcgtagt gtacggatga ggacggcgct
      781 ggcatggagt tccctccggg aaggcacggg ttggcgaacg cggccgggag tcgcattctc
      841 ggtacctact gaaagtacgc actcccgctg gaaaggggaa actgcgatgt acgatgagct
      901 ttccgcgctg tacgacctgt ttgtggactg gccttcgcgg ctcaggaacg aaatgccgct
      961 tctggagaag ctcggtgtgg gagtgccggg ccagcgcatt gttgatgtcg cgtgcggaac
     1021 cggacaccac gcgctggcct gggcggagaa agggtgcacg gtgcgggcat ttgatgcgtc
     1081 acgtgagatg gttgagcggg cacgcatgcg ggaccgggcg ggcctggtga gctggggggc
     1141 agccggcttc gcggaaatcc ccaaggacgg gtgggcggac gcgctggtgt gccttggaac
     1201 ctcgcttccg cacgcggaat cagaggcggc gcaccgcgcc gcgcttgccg cattcgcgag
     1261 ggcgctgaaa ccgggcggcg tgctggtcgt gcatagccgc aaccttgcgc gcactgccgg
     1321 gggaggagaa acggagcgat ttcttccacc tctgccgagg aaacgggatg ggatgaatgt
     1381 tctgttctgg cggttctacg acgttctgtc cccctcactt ctggatttcc atctcgtcat
     1441 tctggaggaa acacccgaca agtggacgca ccgggtgctc acttcccggc tgtgcgtggt
     1501 tcccgcagag caactggcgg cgatggccga ggaagccgac ttccatgaac tccgcgtact
     1561 cggaagcctg gatggcaggc cgtatgaccg cgggaactcc ccggatcttg ttttgaccgc
     1621 gaaacgcagg taaccccaaa cctgggactg tgccgccggt gccggttggc agtatttttc
     1681 aggacgatct tgtccccccg ccgaccgttt tctcgctgaa ggagcaacag atgccgaagg
     1741 ggcagagatg gaacgctgaa tggtcaacag aggccgaccg gaccaccggt ttcgaggtac
     1801 gccggttgac gaacaaccca ccctcttcca accgccattt ctactttcat cagataagct
     1861 ggactcccga cagttcgcac gtgattttcg tgtcagatcg gggggaaggc tcccagcttt
     1921 gcagcatttc catggcgaac ggggagatca ctcaactcac ccaggacggg atggcacatg
     1981 acgccacgat gggcctccat gcgccggttg cgtactacta cacgggtaat gagttccggg
     2041 cagtgaatgt tctgacgctg gaggagcgta cggtcggcgt gttgcccgag aagatgaagt
     2101 gttattccgg gccttcggaa aacgcggacg gcacgttgct cgtgttcgcg gcgcagatcg
     2161 agggcgggag gtgcatggcg aagacagtaa tcgcctcggg agtgacgtcg gtgatccttg
     2221 agacgagcac cgttctgagc cacgtcaact gctcgcttac ggacccggaa tggatcatgc
     2281 actgcgattc cacgcggggt gacgacaagc cgaagcaacg cgtgtgggtg ctttccactg
     2341 acggcatgcg ccactggcac ccgtacacac agacgcctca ggaatggctt acgcacgagt
     2401 cgtggctcgg catgacgggg aaactgctca tctgttactg gcccactgga atcatggaaa
     2461 tcaacgtgga cggcaacggg gcacgcctca ttgccgcagt gaatgcctgg catgcggccg
     2521 ccagccgcga tgggcgctac tgcgtggtgg acaccaactg gcctgaccgc ggaatccacc
     2581 tgatagagcg ggaaacgggc cgcatgtgca aagtgtgcga atctgattcg ggagcaggag
     2641 actcacgcgc ggggagcgag gtgcacccgc atccttcgtt cagccccgac gggaagtaca
     2701 tcctgtttgg ctcgcagaag accggaaaac cggaggtgta cgtcgtcggt acggaacagg
     2761 tgatcgcgcg gcaggacctg tggtggaagc cggagtacca ctggcaccga tggtagcaga
     2821 ggggcgggtg acgattgaac cgctctcgga gcagttttcc cgcgccgcgg gagcgcttat
     2881 ccggacggtg cttcaggagg agttcggcgc cgcgatccgg gcgtgcgaag atgcagatct
     2941 ggaagacgtt ctctccgcgt acgcaggtcc ggagagcgcc gcgtgggtgg cgcaccgcga
     3001 cggcgtggtg tgcggcgtgg ctgccgtgcg cgagaccgag agcggcgcgc tggaactgaa
     3061 gcgattctac gtgcgcgctg agtgccgcgg gaagggcatt ggcggcacac tcctgcgaga
     3121 ggtggaacgc cgcgcggcag aggacgggta ccagcagatc gtgcttacga cgacacacct
     3181 tatggcgcgg gcgcaggagg tgtaccggca cttcgggtac caactggata gtacggaacg
     3241 caagggcgcg gtgacgcttc acacgtaccg aaagcggctt gtgcgggcag cactcggggc
     3301 acgaagactg tccggacctt tgccaaacac gggcacgggt gagattgcgg tggtggtgga
     3361 acggccccgc ggatcgtgtg aggggtggca ctgggaccgg cagacacaca ccctggtatt
     3421 aacggagtat tacgacagac cggtccccgt gaactacggc ctggccccgg agtgggtgaa
     3481 ccccgcggat caggatgcgc tggacgtcat tctgctggac gaccgccgga tgcaaccggg
     3541 ggaaacgctc gtcgccgtgc cggcaggagt gcttctccgt ccggacggcg acgacaagct
     3601 gctggcggtt ccggcggggg gtgcggtgcg gccggttggc cttgatgaac ggtcgcgggc
     3661 acgtgtttct gcgtggtggg atgacgccca tcagcctgtc ggatgggaag gtgaggaagg
     3721 catcgaccgc ctttttgcgc gatgtcggcg gcaaggagca atgaatgggt aagcgaatac
     3781 cgaagcccga atggttcgtg tgcgaaacct gcggcaagca gacgcgggca ttgcgccgtg
     3841 atgtgctgga cgagcactac aatgccctcg ggaagactcc tctctggaac tgcgaagaat
     3901 gctaccaaca gaagaggcgc gagcgcctca gccagcccgg ctcacccgac ccgtcgtcct
     3961 gatcggcttc atgggcgccg gcaagacgaa ggtgggcaaa atcgccgcgc agtatatcgg
     4021 gtgcgatttt gtggacaccg accacatcgt agagcgctcc gccggcaaga caatcccgca
     4081 gctttttgcc gaaatcggcg aagagaggtt ccggcacctt gaaacggaag ccatcgagca
     4141 cgcgctgaac acgcacggcg gcatcatcgc actgggcggt ggagctgcca cgcgtgagga
     4201 gaactggcag ttgctgcgca ggtcaggagc catgacgatc tacctgcagg cctcgccgag
     4261 aacaatactc gcacgggttg tggacaaggg tacgcgaccg cttctggccg gcctcgaccg
     4321 ccagcagatg ctggaaaaga tcacgtccct gctggaacag cgcgaaccgt ggtacctccg
     4381 ggccgatatt acccttccga ccgacaactc gcttgacaag tacggcatgg cggattcact
     4441 ggtcgcgcgc ctcaaagaag ccggcatcag cgcggcggaa tcgcaccgga cgtcatgaac
     4501 ggacggcaga aaaccacgct ctggcgggaa catgttgcgc tctggcggga cgcctggcgg
     4561 cgggtgattg cacaccgtcg gcaggtcgtg ctgatcactc tgttgctgtg gctgccgtgg
     4621 cggtttgtgg ttccgctggt cgaccaccgg ctcggtttca gtgcgttcgc cggatttccg
     4681 cttgaatgga ttgcggacgc gctgtatgat ccattgtacg gagccaccat ccttgcggtg
     4741 ctggcggaaa tgtatccgat ttcggttcgg agcgctctcc tgagggggtt ctggcttgtt
     4801 cctcgtctgt ggctgattga gatcaaggta gcgatctggg ggctggcggt gccctacctg
     4861 atcctccatc tcggggcggc gatcgtgttg acgctgtggc cgagttacgg tgccgcccgc
     4921 gcgctttttg cggtgacatt tgtcagcatg gcgtgggtgc tggtggtggt ggttcggtac
     4981 atgctggcag ccgcggtgat ggttgcggaa acgcaggggc acacctggaa cgaggagcag
     5041 gcgccttcca ccgggtgggc gctcttcgcg gcgcggcagt tgatccgggg gaacgtaagg
     5101 caagcggttc tcgtgatggc ggtggggcag ggggttatct cgcttgtcgt gtccctcctt
     5161 ccttcgggcg atacggtgcc ggcgcagttt ctgaacgcga taggcatggt ggttaccgcg
     5221 ctctggtggg cgcttttgtg gagctttggc cagttctgtc tcagcagagc gcgcggggtg
     5281 accgcacagg aggcaacata tggacgtcag aaagctcgtt gatcatctcg accgccgccg
     5341 catggtgctg tgccacaacg ggacgatcta ttcgcaggca ctgttcggtg agctcacccc
     5401 ggatgaggtg tgcccgggcg tgttcgccta tgcgccggga tggggagtgc tggacccggg
     5461 gatgaccatg gaggcgcacg agcacccgat cgctgagttt tacgtgttcg tgagcggaaa
     5521 gggacggatg cggctgggcg acacgtggtt cgatgttgag ccgaatatgt ccgtgaatat
     5581 cccgcccgga gtggaacatg agctcgcgaa tgacgccaca tcgcctctcc cactcgtgtt
     5641 tgtttcggtc ggcctgaggc tctgacggtc gcgccggccc ctgccagaat agatccgcgt
     5701 tcctgattcc ccgctttgtc tcttgacaag tcagccttcg tcgtgtatcc tcattatgaa
     5761 catatgctca taactcagga ggcgacaccc tatgccgaga ccacggaatc cacgccaggt
     5821 gcgatgcata ccgtgcacgg attacttcaa accgcggggc atcccgttgc gggcgctgga
     5881 agaggtaacc ctggcggtgg aggaactgga agcgttgcgg ctggcagatc tcgaaggcat
     5941 ggagcaggtg gcgggagcag agcagatgga ggtatcgcgc cccacatttg cacgggtgct
     6001 cgcctcggcc cggcagaagg tggcggatgc gctggtgaac gggaaagcgc tccggattga
     6061 aggcggcgtg ttccggatgg cggagaatgt ccctctctgc gggcggcacg ggcacggttg
     6121 gggacagggc cgcggcggac ggtgcgggcg gcgattccgc ggagggcaag ggtttgacgg
     6181 gggtgaggcg ccctctgaac agagtgcctg attccggata agaggtaact gcaaatgtat
     6241 acaatcaatt atgatctttg cacacggtgc ttttcctgcg tgaacgcctg caaaacaccg
     6301 ggtgcactgg aaatccgaga ggacaaaccg aagatcaatt tcgtgttgtg caccgagtgc
     6361 ggcctgtgcg cggaagcgtg tcagcacggg gcgataacga aatgggaagc gcgcgaggaa
     6421 gatgtccggc catcggaagc cccggttgcg agttcaccgt cgcctgtttt tactcccgct
     6481 gcgaatccgt tttcccgggg atttgcgaga tgcggggcat ggatccgttc ggcgtgtgcg
     6541 cggcggggag cgcccggtgg ttaccgaagg ggcggacgct ggagggtttg atatcccggg
     6601 agcgttctgg ttcgaaacga ttcacaggaa aggaaggact tcatgaagat cgcggttccg
     6661 gtagaaacga acgagggcct caaaagtatc gttgccggcc atttcggaca tgccccctat
     6721 tacgcgtttg tggagggcaa gaccaggcat tgcgtgatcg ttccaaacgc gagttcgcac
     6781 aatggcgggg acaaactgcc cccggaatgg ttgttcgatg aaggc
//
LOCUS       DAOSOO010000172         6820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11213_length_6820_cov_4.537324, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000172 DAOSOO010000000
VERSION     DAOSOO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11213_length_6820_cov_4.537324"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..604
                     /locus_tag="PLT86_11085"
     CDS             326..604
                     /locus_tag="PLT86_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37196.1"
                     /translation="MTLYNQPNAFGITGISQCPIYDPGCGSREDWFGLSSESPVCADM
                     SRIAELAALDLRSRGKRRNLRKDDDREVVLSKERLAQGWFHRSERDRN"
     gene            complement(677..1300)
                     /locus_tag="PLT86_11090"
     CDS             complement(677..1300)
                     /locus_tag="PLT86_11090"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT37197.1"
                     /translation="MPLPVLSLASATAALRGGEVVAYPTETFYGLAVLPSDAEALARL
                     VRLKGRDTDKPVSLILGKAEVVSDFATSLTPTTIRLISLAWPGPLTVVLPAKDSVARV
                     ITGGTGTVAVRVPSHPGARMLADSAGGAITATSANRQSMPPARTAEEVEAQFPDGLAG
                     VFNGERAPGDLPSTLVRPVGGTLHVLRRGAVDLPTLREWWSGEIVDA"
     gene            1559..2323
                     /locus_tag="PLT86_11095"
     CDS             1559..2323
                     /locus_tag="PLT86_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT37198.1"
                     /translation="MSVPLGDFVNYLNEYLRIDEIPDSDEALNGLQVEGAADIRRVAV
                     AVDACLATIAGAALWGADIMLVHHGLFWGPKAPLTGGQYRRFSALVRHRIGVFSAHLP
                     LDLHPEIGNNMMLAKAIGLQVTGTFCRQNGVDVGIFGRLEVDRSDLVQRIEAALEIDS
                     VPHVLEHGLWQTSTVGIITGGGGRFVKEAAHAGIDTFITGEGPHFSALDAEEFGMNVI
                     HAGHYATEKLGVKALAAHVADKFGVETQFIDHPTGI"
     gene            complement(2370..2846)
                     /locus_tag="PLT86_11100"
     CDS             complement(2370..2846)
                     /locus_tag="PLT86_11100"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOT37199.1"
                     /translation="MLIADRVTGYEEWEERVLAGETGALLDRILSAPGVNLAAEPVCV
                     TALVACRSRRGTLPGTTEIRACGARLQALVTVIQPVIIVALGLCTGKALVRSGIAEPA
                     IDGRDYDVFRGIPVRTTCHPAEALWGVPKEVNRIKRILYQDWKAISAHYFALKTTR"
     gene            complement(3005..3664)
                     /gene="rpe"
                     /locus_tag="PLT86_11105"
     CDS             complement(3005..3664)
                     /gene="rpe"
                     /locus_tag="PLT86_11105"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT37200.1"
                     /translation="MLLPRTRLIAPSLLAADFGDLRNEISRVEKAGADWLHLDVMDGH
                     FVPNLTFGPFIVSAIRSLTPLPLDTHLMITDPAFFAPRFAESGSTLITFHVEAVQDVA
                     RTIREIKALGVNAGLSIKPATPVDVVLPYLESLDLVLVMSVEPGFGGQKFMPEAVPKI
                     RSLREAIDARNLPTKIQVDGGINPETAKLVRDAGVDVIVAGTSVFRSTDPAAALAQLR
                     A"
     gene            3944..4165
                     /locus_tag="PLT86_11110"
     CDS             3944..4165
                     /locus_tag="PLT86_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37201.1"
                     /translation="MGLLYERDRRVTKDKNLPEIKETDTMKRSGLVVLPTREHDIEEF
                     TEEWDELDLFEDEIVDEDVDPEEVDEEAF"
     gene            complement(4300..5139)
                     /gene="iolB"
                     /locus_tag="PLT86_11115"
     CDS             complement(4300..5139)
                     /gene="iolB"
                     /locus_tag="PLT86_11115"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOT37202.1"
                     /translation="MPETQTDFHIRKTLLHGENVIVPANHPSLELLRFSLLCLNEKES
                     HSFCLDEEEAALVILTGTASIKIDDSTYERIGGRRDVFSGNAATVYAPCHSKIVVDCA
                     GPGPLSLAVCCSKSTLRREPALIAPQDVKTKTVGRANWTRHVKDIIDVSFDAGHIVVG
                     ETINPPGNWSSSPPHRHEVDNPPDEVKMEEIYFFQLKPQQGFGFQRIYTDDRSLDVSY
                     AVEHNSTVVLPKGYHPVAAAPGYQLYYLWFMAGPTSRTLCPKDDPAHSWLKAAEPIID
                     GLK"
     gene            complement(5157..5984)
                     /locus_tag="PLT86_11120"
     CDS             complement(5157..5984)
                     /locus_tag="PLT86_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT37203.1"
                     /translation="MVTLTGFGDEISPDLTEQMDVMESEGIRHIELRAVWNTGVHKLS
                     EEQRQKIKEETQRRGFRISSLGSGLGKIKVDDDFGPHVRDCEVLIQTAKFLGTPYIRI
                     FSFYVPQDQPVTAHRDEVMRRVRVLTDMAAAEGIVLGHENERHIYGEKPEQCRDLLDT
                     INSPSLRAIFDPANFVQAGVRPFEDAWPMLKDDVLYFHIKDAKQADGRVTPAGEGDGG
                     IKAILADKLASGWEGFLSLEPHLKIAGPAGGYSGPLAFKTAAQALKRLLDEIGVRYH"
     gene            complement(6017..6658)
                     /locus_tag="PLT86_11125"
     CDS             complement(6017..6658)
                     /locus_tag="PLT86_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37204.1"
                     /translation="MRFPQAVTFKGTMRVISYDPRAGMRLERMPESRFRLWFGLFFSV
                     GLACIGVLLFARTAFENYRASGALNWLDFLLIVIGLICVAFGLIVALPLALRFVPKVI
                     TTTHEPPLVCLRQFRTHTWRPQDVRGLHLVGRQYLRRTDPDAPSSWYVFSLVLQKSKG
                     RDRLLLQTLPQPDYEAGFQDIVPIATYFSELLATPLTTVCNWDAPPHITRRRT"
BASE COUNT         1478 a         1867 c         2031 g         1444 t
ORIGIN      
        1 gtgcattcct agtatagagc cagaccgaca actggaggaa gtgctctgtt tgctgcgaat
       61 ccgccggttc gcgccacacg atgcgctaat ggcattaatg tgcaattatt gcgcaatcgg
      121 gtgttttggg gtcgcgccga caagcgtcgt gatgtccttt ttatgggctt caataatgga
      181 agtgcaacaa tggcacactc attgcatact ggaattccgg gttcttcgag gcgcaaggga
      241 taaccggagc acggaacccc ggaagcgccc gtcgtcacaa gcgacggcga acaacgagcg
      301 aaaggaaaac tcaaaggagc gaacaatgac gttatacaat cagcccaatg cgttcgggat
      361 caccggaatc tcgcaatgtc cgatatacga ccctggctgc ggttcgcggg aggattggtt
      421 cgggctgtcg tccgaatcgc ccgtgtgcgc cgacatgtca cgcatcgcag agctggcagc
      481 cctcgacctc cggtcaaggg gaaaaagacg caatctcagg aaggacgacg atcgggaagt
      541 cgttctttcg aaagagcgtc tcgcgcaggg gtggtttcat cgatcggaac gcgaccgaaa
      601 ctgacatgaa cgctctactc gaaggggaag gaaccgtggg ctgtgtggca cggctccttt
      661 actttttctg gatgctctac gcatccacga tttcgccact ccaccattcc ctcagagtgg
      721 gcagatcaac agcgccccgc cgcagcacgt gcagtgtgcc cccgacggga cgcaccaggg
      781 tggacgggag gtctcccggt gcgcgttctc cgttgaacac cccggcgagg ccgtcgggga
      841 actgagcttc cacctcttcc gccgttcgcg cgggcggcat actctgacgg ttcgcgctcg
      901 tcgccgtgat ggcacctccc gcggaatcag cgagcatcct cgcaccgggg tgagaaggta
      961 cgcggaccgc gacggtacct gtcccgccgg taatcacacg cgccaccgag tctttcgccg
     1021 ggaggacgac cgtaaggggg ccgggccacg cgagagaaat cagacgtatg gtggtggggg
     1081 tcaaagaggt tgcgaagtcg gataccactt cggccttccc gaggataagg cttacgggtt
     1141 tgtcggtgtc tcttcctttc agtctgacga ggcgggccag cgcctccgca tcggaaggga
     1201 ggacagcgag cccgtagaag gtttctgtcg ggtaggccac tacctcgccg ccacggaggg
     1261 cggcagtagc tgatgcaagg gacaaaacag gcaatggcat gttacgtgcc tctcgatctg
     1321 cacaagggtt tcctgaagga cagggaattg acattaccgt gatcaaggag taacattcgc
     1381 aagtcttgat aatacggaca agggcttccg gagtgtggag catcctacgc gcgtgaccgg
     1441 tacgattgcc ggctccgagc gcggtgaggg aaggtctgaa ttccgtgtct gttcataacg
     1501 atgtgggaag ttcccgtttt gttgggggcg gggccgtttt gcaggagcga aggttactat
     1561 gagcgtccca cttggagatt tcgtcaacta cttgaacgaa tatctgcgga tcgacgagat
     1621 acccgactca gatgaggctc tcaacgggct tcaggtcgag ggtgctgcag atatccggag
     1681 ggttgcagtc gccgttgatg catgcctcgc gaccatcgca ggtgcggcac tttggggcgc
     1741 ggacatcatg ctcgtgcacc acggactttt ctgggggccc aaggcgcctt tgaccggggg
     1801 gcagtatcgc cgtttttccg cactcgtccg ccaccgtata ggtgttttca gcgcgcatct
     1861 cccgctggac ctgcacccgg aaatcgggaa caacatgatg ctggcgaaag cgataggcct
     1921 gcaagtaacc ggtaccttct gcaggcaaaa cggagtggac gttggaatat tcggccgcct
     1981 ggaggtggac agatccgatc tggttcagag aattgaggct gcactggaga ttgattccgt
     2041 gcctcatgtc ctcgaacacg gactctggca aacgtctacg gtcgggatta ttaccggcgg
     2101 cggcgggcgc ttcgtcaaag aagctgcgca cgcgggaatc gacacattca tcacgggtga
     2161 ggggccgcat ttttccgcat tggacgcgga agagttcgga atgaacgtga tccatgcggg
     2221 tcattacgcc acggaaaaac ttggtgtgaa agcgctggcg gcccacgtgg cagacaaatt
     2281 cggcgtagaa acccagttca ttgatcatcc caccgggatt tgacggtttc acggccccga
     2341 cgcttcgaga acaccgtgca tattttgcgc taacgcgtcg ttttcagcgc aaaatagtgt
     2401 gctgaaattg ccttccagtc ttggtacaaa atgcgtttta tgcgattgac ttctttcggc
     2461 accccccaga gcgcttcagc cgggtggcat gtggttctga cggggattcc ccggaagacg
     2521 tcgtaatccc ggccatcaat agccggttcg gcgatgccag aacgaaccaa cgctttgccc
     2581 gtgcacagac caagtgcaac aataataacg ggttgaatca cggttacaag agcctgcaat
     2641 ctggccccgc aggcccgtat ttccgtggta cccgggagtg tgcctcttct gctccggcag
     2701 gcgacgagtg cggtcacaca tacaggttct gccgcgagat ttactccggg ggctgagaga
     2761 atacggtcca gcagcgcacc ggtttctccg gcgagaaccc gctcttccca ttcttcgtac
     2821 cccgtgacac gatcggcaat cagcattaac ccgccgcgct tgaagcggga ggcgcagaga
     2881 acacgtctgc gggtatccgc cagactgcat ctgtgacacc gggcgaattc gcggtgtagc
     2941 tccgaaaggc tcccgggaac ggcagccgct tcgagaagag gggaatcggt caacagcacg
     3001 gatatcacgc ccgaagctgc gcgagcgccg cagcggggtc ggtgctgcga aaaacggagg
     3061 tgccggcgac gatgacgtct actccggcat cacggaccag cttcgccgtc tccgggttta
     3121 tcccgccatc aacctggatt tttgtgggaa gattgcgggc gtcaattgcc tcgcggagcg
     3181 agcgaatttt cggtaccgct tccggcatga acttctggcc tccgaacccg ggttccaccg
     3241 acattacaag cacgaggtcc aggctttcga gatagggcaa caccacgtcg acgggtgttg
     3301 cgggttttat gcttaacccg gcgttcactc cgagggcctt gatctcgcgt attgttcgag
     3361 ccacatcctg aacagcttcg acgtggaagg tgatgagggt gctgcccgat tcagcgaaac
     3421 gtggagcgaa aaaggcgggg tcggtaatca tgaggtgggt atccagcgga agcggcgtga
     3481 gactccgaat cgcggacacg atgaaggggc cgaacgtgag atttgggaca aaatggccgt
     3541 ccattacgtc gaggtggagc caatcagcac cggccttttc aactcgggag atttcgttgc
     3601 gcagatcgcc gaaatcggcc gccagaagag atggagcaat gagacgtgtt cgcgggagca
     3661 acatgaacat gttcctcggg attgggggcc tacggacacc tacgggagaa ataaaaatac
     3721 ggcttcattc gataccgcgc acagtggctc ttgacaacgc gcttactatt gcatacgatg
     3781 gttcgtgtcg agaaggaccc agaagcggct caaggcaaca gatttcgcgg tggtgccaag
     3841 gcgaagaaag gtccgtggca agaactgctt caggcaagcc ttttattggc gtggcttttg
     3901 cattacgcga cgcgcagata atgtcgacga aggaggaaag ccaatgggtt tgttgtacga
     3961 gagagatcgc cgggtcacaa aagacaagaa tcttccggaa atcaaggaaa ccgatacgat
     4021 gaagcgttcc gggctggtcg ttcttcccac ccgtgaacac gacattgagg aatttacgga
     4081 agaatgggac gaactcgatc tgtttgaaga cgaaatcgtg gacgaggacg tcgacccgga
     4141 agaagtggac gaagaagcgt tctagcccgc aggggttgcg agtctgagga cgcaacccca
     4201 ctgcggacgt cgaatggcgt ttgagtcggt gtttgaaaag taatggtacc aaccgcttcc
     4261 cagcgttacc gtcaaccccc tccttttgtt cttctttgct tatttgagcc cgtctattat
     4321 cggttcggca gctttcagcc agctgtgcgc gggatcgtcc ttcgggcata acgtcctgct
     4381 cgtcggtccc gccatgaacc agaggtagta aagctgataa ccgggtgcgg cagccaccgg
     4441 gtggtatcct tttggcaata cgactgtgga attgtgttcc accgcgtagc tgacgtcgag
     4501 agaccggtca tcggtataga tccgctggaa gccgaatccc tgttgcggtt tcaattgaaa
     4561 gaaatagatc tcctccattt tcacttcgtc cggcgggttg tccacctcgt gcctgtgcgg
     4621 tggactggaa gaccagtttc ctggcggatt gatggtttcc ccaaccacga tgtgtcccgc
     4681 gtcgaatgac acgtcgatga tatctttgac gtgccgcgtc cagttcgccc gccccaccgt
     4741 tttcgttttc acgtcctgcg gggcgattaa tgcgggttca cgccgcaacg ttgatttgga
     4801 gcaacagaca gccagagaaa gaggtccggg gccggcgcag tcgaccacga tcttgctgtg
     4861 acatggggca tacacggtgg cggcgtttcc ggagaataca tcacgccgac cgccgatccg
     4921 ttcataggtg gaatcgtcga tcttgatgga cgcggtccct gtaagaatga ccagagcggc
     4981 ttcttcttca tccagacaga acgagtgtga ttccttttcg ttcaggcaca gcagcgaaaa
     5041 acggagcaac tcaagcgacg ggtgattggc cggaacgatg acgttttctc catggagcaa
     5101 cgtcttgcgg atgtgaaagt ctgtttgagt ttctggcatc tatttcctcc tggcatctag
     5161 tggtaacgca cgccgatttc gtcgagaagt cgcttcagtg cctgcgcggc ggttttgaag
     5221 gccaatggtc cggaataacc ccctgccggg ccagcgattt tcaggtgcgg ctccaacgag
     5281 agaaagcctt cccatcccga cgccagtttg tcggcaagga ttgccttgat tcctccgtct
     5341 ccttcgcctg cgggtgtgac acgtccgtct gcctgtttgg catctttgat gtgaaagtag
     5401 aggacatcat ccttcagcat cggccacgcg tcctcaaatg gtcgcacgcc agcctgaaca
     5461 aagttcgccg gatcgaaaat cgcccgaagc gatgggctgt tgatagtatc gagcagatct
     5521 ctgcattgct ccggcttttc cccgtagatg tggcgttcat tttcgtgccc gaggacgatc
     5581 ccttcggcgg cagccatgtc ggtcagcacc ctgacccgcc gcatgacttc atccctgtgg
     5641 gcggtcaccg gttggtcttg cggaacgtaa aagctgaaga ttcgtatgta gggcgtgccg
     5701 aggaacttcg ccgtctgaat aagtacctcg caatcacgca cgtgcgggcc gaaatcgtca
     5761 tcaaccttga ttttgccgag cccggaaccg agcgaggaaa tgcgaaatcc gcgccgctgc
     5821 gtttcttcct tgatcttctg gcgttgttct tcgctgagct tatggacacc tgtgttccag
     5881 accgcccgta actcgatgtg gcgaatccct tcggattcca tcacgtccat ctgttctgtc
     5941 aagtcgggcg atatttcatc tccaaacccg gtcagagtta ccatgttcga gtatcctcct
     6001 ttgcgaggga caaaccttac gtgcgtcgcc gggtgatatg cggtggcgca tcccagttac
     6061 acacagtggt gagcggagtt gcgaggagct ccgaaaaata ggtcgcgata ggaacgatat
     6121 cctgaaagcc ggcttcgtaa tcgggctggg gcagtgtctg cagcagaagt ctgtcccgtc
     6181 ccttcgattt ctgcagcacg agactgaaca cataccagga ggaaggggcg tccgggtcgg
     6241 tgcgcctgag gtattgtcgg cccacaagat gcagaccgcg tacatcctgc ggccgccacg
     6301 tgtgggtgcg aaattgccgc agacaaacga gaggcggctc gtgtgtagtg gtgatcactt
     6361 tcggcacaaa tcgtaatgcg agaggcaacg cgacgatcag cccgaacgct acacagatga
     6421 ggccaattac tatcagaagg aaatcaagcc agttaagagc tcccgaggca cggtagtttt
     6481 caaacgccgt tcgcgcgaaa aggagcacac cgatgcacgc aagcccgacg ctgaaaaaga
     6541 ggccgaacca caggcgaaac cgcgattccg gcattcgttc gaggcgcata cccgcacgcg
     6601 ggtcgtaaga aatcacccgc atcgtgcctt tgaacgtgac cgcctgagga aaacgcaagc
     6661 ctcacccccc cacagcagtt attgaggaca actgccgctg ggcatttaca agcttcgcgt
     6721 acacaccctc tttgagcagc aattcgtcgt gcgttccgaa ttcgaccacc tgtccgtcct
     6781 cgattacgag caggcggttt gcgttcttca gcgtgctgag
//
LOCUS       DAOSOO010000173         6773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11317_length_6773_cov_5.176838, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000173 DAOSOO010000000
VERSION     DAOSOO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11317_length_6773_cov_5.176838"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLT86_11130"
     CDS             complement(<1..373)
                     /locus_tag="PLT86_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT37205.1"
                     /translation="MSRSFRAFAAAALAASPAAAVGPLSFDVLWTHETNSQVRGCPGL
                     VDTPEGPLVIVGTDNGDILALNGNGQVRWSVKIDARAHGWPTTIDLPAGKTVLIGDSD
                     GTLHAFAPDGTLRWRVSMGNLS"
     gene            complement(863..1684)
                     /locus_tag="PLT86_11135"
     CDS             complement(863..1684)
                     /locus_tag="PLT86_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT37206.1"
                     /translation="MLIDTHAHLDFEDFEKDLDETVRRAIDAGVGIMINPGINVPSSR
                     KAVAIAELFDCVYAAAGIHAHDVADCSPDVIDEIARIAEHPKVVAVGECGLDFFRNYA
                     PRARQLEVFRDQVRLALRLGKPLVVHSRGAEEETLQVLTEERAELVGGVLHCFGGAPD
                     QALRAQILGFVVGVGGTLTYPRSDRRDFVANVDIHSIVLETDAPFLPPEPHRGKRNEP
                     AWLPLVAQKLAETRSLPLDDVHRITSSNAERLFRIGMADPARTSSFPSATAPRSG"
     gene            1869..3323
                     /locus_tag="PLT86_11140"
     CDS             1869..3323
                     /locus_tag="PLT86_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37207.1"
                     /translation="MSIRVDDTDLHIINMHTRMPFRYGIATLTAIPHLFVRVFATIDG
                     VRSSGIAADGLSQKWFTKNPDAAYEDEIADMLSVIRSACSLATQIEQAPTVFGFWHVL
                     YRAQAEWARETSFPPLLWGFGVSLVERALIEAYCRANRVSFYQALKANAFGIALEEIH
                     PELRGRLPGEFLPERPLRQIVVRHTVGLADPLTESDIIAGEALNDGLPHSLEACIREY
                     GIKQLKIKLSGDCEADVARVERIFSVVRAWAPANVRFTLDGNENYHAVETFQEFWRVL
                     AERSSLRGFLQNMLFVEQPLHRDVSLGDTVKTAFADWKDRPPVIIDESDATLDSLPRA
                     IECGYRGTSFKNCKGVFKGIANACLVRFLNEKRKGVAFIISAEDLSNVPPVALQQDLA
                     SLSALGISNPERNGHHYFRGMAAFPESVQTAVLDAHGDLFYGHERGFAALRIRDGCVS
                     TDSVVEAPYGRACEFDPGCFTPVDEWRFDSLSGS"
     gene            3452..4489
                     /locus_tag="PLT86_11145"
     CDS             3452..4489
                     /locus_tag="PLT86_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT37208.1"
                     /translation="MQRRRGAIPRVFDPMVFFRELYKSITPMYTYSAKDPSEHARWER
                     GLRRKFVELLGGFPATKEPLSPEVSEIVEFPDYVRERVVFNTRPLMSVPAYLLRPNKP
                     ISGKLPAIVCLPGHGPGKNTIVGFDDKGEPRQEIGGYQADFAIQAVRHGYVALAIEQL
                     AFGERNSPDPRCRGCNEPSMNALMLGLTMIGLRVWDARRALDYLLTRPEVDPGRIGVM
                     GISGGGTTTLYTAAVDKRFKAAVVSGYLCTFRDSIMAMDHCCDNFIPRIIRYAEMYDL
                     AALVAPRALFAESGTKDSIFPIDAARFAFRKAKRAWDVLGVPDKIGHEVFEGEHQFWG
                     KGAFEFLKRNL"
     gene            complement(4549..5526)
                     /locus_tag="PLT86_11150"
     CDS             complement(4549..5526)
                     /locus_tag="PLT86_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37209.1"
                     /translation="MSDAAEPLEVSTKLFGWNGMTCQIPFHWDVTSVSGSAKSGYFAF
                     DDEQDRRLEIKYERAGRMRKPDLDRTLSGYFATLKKKAKSGQELRLDDSVRVPGVETM
                     PAEYEVRSYGWEADAITRGLIWHCSTCHRIVIAECRARKNRVNTKEMAQVLTSIRCHC
                     EGDTAPWAVFDFEVLVPRELELGLSRLQAGLISFSFSAKKRRLVVDRLGMGQVVMKHS
                     SLDTYVRDVHYKKLRKIRLRFQPVNWNGHEGFRFEGEHKRLYDWIPALGARIRAWRVS
                     DHIAGLTWFEEPTNRIYIIRAEGPDCMELAEKVAASARSRIAGEIPGNR"
     gene            complement(5618..5788)
                     /locus_tag="PLT86_11155"
     CDS             complement(5618..5788)
                     /locus_tag="PLT86_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOT37210.1"
                     /translation="MTHPELLKRVIGVLVGAVLGFAYYKFVGCSTGACPITSNPWIST
                     IYGGVIGLLITL"
     gene            complement(5785..6207)
                     /locus_tag="PLT86_11160"
     CDS             complement(5785..6207)
                     /locus_tag="PLT86_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOT37211.1"
                     /translation="MAHYAIKAEVNTSFVQAVAATKAALATEGFGVLTEIDVQATMKA
                     KLGKDMLPYVILGACNPPHAFNVISAEPDIGLMLPCNVIVYEKPDGAIVVAAINPEVA
                     MQPAGNDQLRPAAAIVRERLERVVRAVEGTGIRRGQNG"
     gene            complement(6379..>6773)
                     /locus_tag="PLT86_11165"
     CDS             complement(6379..>6773)
                     /locus_tag="PLT86_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT37212.1"
                     /translation="ERVDDLPLLVHRLLSRIADRHGSAAPGVTDDVLRLFRSYRWPGN
                     VRELQAALEYAIIRSQGSTIDVHHLPPSIVQPSELAVARNCILDEQAIRMALEKHRYN
                     RTAAAKELGISRITLWRRMRDWDIAPRR"
BASE COUNT         1378 a         1980 c         1930 g         1485 t
ORIGIN      
        1 tggaaagatt tcccatggaa acgcgccacc gaagcgttcc gtccggggcg aacgcatgca
       61 gcgttccatc gctgtcgccg atcaggactg ttttgcccgc cggcaggtcg atggttgtcg
      121 gccacccgtg agcgcgcgcg tcaattttga cgctccaacg cacctgacca ttaccgttca
      181 gtgcgaggat gtcgccgtta tcagttccga ctattacgag cggaccttcg ggtgtgtcaa
      241 caagccccgg gcaaccccgg acctgcgaat tggtttcgtg ggtccacagg acgtcaaacg
      301 acagcggccc gacggctgct gcgggggagg cagctaacgc cgccgcagcg aaagcgcgaa
      361 aagatcgact catttgcagt ccttcctgag agctgaaccg atgggaaccg aagggaggat
      421 aacgcgcgga tttcttaagc aatcgaaatc aagggtaagc ctgagggacg cgcccggtca
      481 acgcctgtcg gggcttatcc ggttgcgaag tcgttgcaga aagcaggggt cagtcgacgc
      541 gcttctagcc ctcgtttgcg ggttccaaca ggagtggcgc gtgttccgtc ccacccagcc
      601 cattgaagga aatctcgccg agaactccat tgaagcgtgt tttttggatc gcatctctga
      661 ttcggccaca acgaagtgtc agttgcagct gccgctgcgt ttcggcgttc gcttctggac
      721 gtgtaacaga cccctccatc ttcggccgcg cgtgttgcaa tcgcctggcc ggataatgcc
      781 ggatgatctg atccacgcga gcgtttcccg ggaatgccgt tgtccttccg cccccatcgc
      841 ttcgcgatcc gttccaagga tgtcatcctg agcggggggc cgtcgcggac ggaaatgagg
      901 aggtgcgtgc ggggtccgcc atcccgatcc ggaaaagcct ctcggcgtta ctggaggtga
      961 tccggtgcac gtcatccaac ggaagtgacc tcgtctctgc cagcttttgg gcgacaagcg
     1021 gtaaccacgc gggctcattc cttttgcctc ggtgcggttc tggaggcagg aaaggcgcgt
     1081 cggtttcgag aacgatgctg tggatatcca cgttggcgac gaaatccctc cggtccgaac
     1141 gcgggtaggt gagtgtgccc ccgacgccaa cgacaaatcc cagtatctgc gcacgcaggg
     1201 cctggtcggg ggcaccgccg aaacagtgaa gcaccccgcc gaccagttcc gctctctctt
     1261 cagtcagaac ctgaagggtc tcttcctctg ctcctcgaga gtgcaccacg aggggctttc
     1321 ccagacgtaa cgcgagacgc acctgatcac ggaagacctc caactgcctt gcccgggggg
     1381 catagtttct gaagaaatcg agcccgcatt cgccgaccgc cacaaccttg gggtgttccg
     1441 caatcctggc gatttcgtcg atcacatctg gcgaacaatc cgcaacgtca tgcgcgtgta
     1501 tccctgcggc ggcgtatacg cagtcgaaga gctcggcaat cgcgactgct ttccggctgc
     1561 tgggcacatt tatccccgga ttgatcatga tcccgacgcc cgcatcgatc gcccggcgca
     1621 cggtttcatc gagatccttt tcgaagtcct cgaagtcgag atgggcgtga gtgtcgataa
     1681 gcatcgtccg cttctatcct ctcctattcg agaatggatc ggttacaaag gtgaaatatc
     1741 gtccgaacga aagccaaggt cttgcacgcg aatacatgcc tccgggttga tcttcgagcg
     1801 gatattggtg aacttctgcc ctcgaagtaa tcagtccgtc aagctttctg ccctgtaggt
     1861 tatcccgaat gtcaatacgt gtagacgaca cagacctcca catcatcaac atgcatacga
     1921 gaatgccgtt tcggtacggc atcgccacat tgactgcgat acctcatctg ttcgttcgtg
     1981 tcttcgcaac gattgacgga gtgagaagct ccggcattgc tgcggacggc ctttcacaga
     2041 aatggtttac gaagaacccg gacgccgcgt acgaagacga gattgccgac atgctgtcgg
     2101 ttatccggtc agcctgcagc ttggcaacgc agatagaaca ggctcccacc gtgttcggat
     2161 tctggcacgt tctttaccgc gcccaggcag aatgggcgag agagacgagt ttcccgcctc
     2221 tgctctgggg ttttggtgta tcgctggttg agcgcgcgtt gatcgaagca tattgcagag
     2281 ccaatcgtgt gtcgttctat caggcattga aggcaaacgc ctttggcatc gcgctggaag
     2341 aaatacaccc ggagctccgg gggcgtctcc cgggggagtt tctgccggaa agaccgttac
     2401 ggcaaatcgt tgtccggcac accgttggat tggcggatcc gctcacggaa agcgatatca
     2461 tcgcgggcga agcgctgaat gatggtctgc cgcactcatt ggaagcatgt attcgcgagt
     2521 acggaatcaa acaactgaaa atcaaacttt caggggattg cgaagctgac gtcgcacgcg
     2581 tcgaacgtat cttttcggtc gtccgggcgt gggctcctgc gaatgtccgt ttcacactcg
     2641 atgggaatga gaactaccac gcagtcgaaa cgttccagga gttctggcgg gttctggcgg
     2701 agcgttccag tctccgtggt ttcctgcaga acatgttgtt tgtcgaacag cccctgcatc
     2761 gcgatgtctc gctaggtgac accgtgaaga ccgcttttgc agactggaaa gatcgtcctc
     2821 ctgtaatcat cgacgaatcc gacgcgactc tggacagcct gccgagagcg attgaatgtg
     2881 ggtaccgtgg cacgagcttc aagaattgca agggtgtgtt caagggtatt gcgaacgcct
     2941 gtctggttcg ttttctgaac gagaaacgca aaggtgtcgc gttcatcatc agcgccgagg
     3001 acctttccaa cgtaccacct gtggctttgc agcaagatct cgcaagtttg tctgcgctcg
     3061 gtatcagcaa cccggaacgc aacgggcacc attacttccg tggtatggcc gcttttccgg
     3121 agagcgttca gacggctgtt cttgatgcac acggcgacct gttctacggc cacgaacgcg
     3181 ggtttgcggc gctcagaatc cgcgacggat gcgtttcgac agacagcgtg gtggaagcac
     3241 cttacggtcg agcctgtgag ttcgatcccg gttgcttcac gccggtggac gaatggcgat
     3301 tcgattcgtt gagcggttca tgacatcggt cgcgaggggc ttctgccgca cgagcgctcg
     3361 gacacggcga ggcctgaaaa agcgtgcaga accggtcttc tgatcagact tcccctcagt
     3421 tgcgtttcac cgaaatcgaa aggtagcact tatgcagcgg cgaagaggag caattccgag
     3481 agttttcgat ccaatggtgt tttttcggga attgtacaaa tcgattactc caatgtacac
     3541 gtactcggcc aaggacccgt cggaacatgc gcggtgggaa cgcggtctac gcaggaagtt
     3601 tgtggaactg ctcggagggt tccccgccac gaaggagccg ctttcgccgg aggtgtcgga
     3661 gattgtggag ttccccgatt acgtccgcga gcgcgtcgta ttcaacacgc ggccgctcat
     3721 gtcggtgccg gcctacctgt tacgtccgaa caagccaatc agcgggaaac tccccgcaat
     3781 cgtgtgtctg cccgggcacg ggccggggaa gaacaccatc gtcggtttcg atgacaaggg
     3841 agaaccccgc caggagatcg gtggctacca ggcggatttc gcgatccagg ccgtgcgtca
     3901 cgggtatgtt gcgctggcaa tcgagcaact ggcgtttggc gagaggaatt caccggaccc
     3961 gcgttgccgt gggtgcaacg agccgtccat gaacgcgctc atgctcgggc tgacgatgat
     4021 cgggctcaga gtttgggatg cccggagggc gctggattac ctcctcacgc gtccggaagt
     4081 agatccggga cgtatcgggg ttatggggat atcgggaggc ggcaccacaa cgctctacac
     4141 cgcagcggtt gacaaaaggt tcaaagcggc agtggtcagt gggtacctgt gcacattccg
     4201 ggatagcata atggcgatgg atcactgctg tgataacttc atcccgcgca tcatccgcta
     4261 cgcggaaatg tacgaccttg cggcgctggt agcgccccgg gcgctttttg ccgaaagtgg
     4321 aacaaaggac tccattttcc ccatcgacgc tgcgcgtttc gcattccgta aggcaaagcg
     4381 ggcgtgggat gtcctgggcg ttccggacaa aatcgggcat gaagtgttcg aaggcgagca
     4441 tcagttctgg ggcaagggag cgttcgaatt cctcaaacga aacctgtgaa atccgcttcc
     4501 tcacgcgcct gcggtgggtg gaggaggctc cacccacccg caagcggctt atctgtttcc
     4561 cggaatttcc ccggcaatcc ggcttcgcgc tgatgcggcc actttttccg ccagttccat
     4621 gcaatcaggg ccttccgccc ggattatgta gatccggttc gtaggttcct cgaaccacgt
     4681 caatccggcg atgtggtccg agacacgcca tgcccgtatt ctcgcgccca gtgcgggtat
     4741 ccagtcgtac aggcgcttgt gctcaccttc aaagcggaat ccctcgtggc cgttccagtt
     4801 aaccggttga aaacgcagtc ggatctttcg cagcttcttg tagtggacgt cacgaacgta
     4861 cgtatcaaga gacgaatgtt tcatgaccac ctggcccatg cctagccgat ctacgaccag
     4921 gcgacgcttc ttcgccgaaa aactgaacga aatgaggcct gcctgcaggc gcgataagcc
     4981 taattccaac tcgcggggca caagaacctc gaaatcaaac accgcccagg gcgcggtatc
     5041 accttcgcaa tggcaacgga tggacgtcag cacctgcgcc atctctttcg tattgacgcg
     5101 gttttttctc gcgcggcact cagcgatcac aattcgatga catgtcgaac aatgccatat
     5161 caatccccgc gtgatggcat ctgcctccca cccgtacgaa cgtacttcgt actcggccgg
     5221 catggtttct acgccgggaa cgcggacact gtcgtccaga cgcaactcct gacccgactt
     5281 cgcctttttc ttcaacgttg cgaagtatcc cgacaatgtt ctatcgagat caggtttcct
     5341 catccggccc gcgcgctcgt acttgatttc caggcgccgg tcctgttcgt catcgaaggc
     5401 gaaatacccg cttttcgctg atccggacac gcttgtaaca tcccagtgaa aagggatctg
     5461 gcaagtcatt ccgttccaac cgaaaagctt cgtcgatacc tcgagtggtt cagcggcgtc
     5521 gctcattccg atcctgatag tcccctcttt ggttcggcca gcgcaaaaac tgcggcgcgc
     5581 aggcagccca cggatctttg ttgccagtcc cgcgctgtca aagcgtgatg agcagaccga
     5641 tgactccgcc gtatatcgtg cttatccacg ggtttgacgt gatcggacac gcgcccgtcg
     5701 aacaaccgac gaatttgtaa tacgcgaacc cgagtacggc accgacgagg acacctatca
     5761 cacgcttgag gagttccgga tgggtcatcc gttctgcccc ctgcggatgc cggtgccttc
     5821 cacggcacga acaacccgtt cgagccgttc acgcacgatt gccgcagcag gtctcaattg
     5881 gtcgttcccc gcaggctgca tcgccacttc gggattgatc gctgcgacca cgattgcccc
     5941 atctggtttt tcatacacga tcacgttgca cggcaacatc aatccgatat cgggttccgc
     6001 ggatatcaca ttgaatgcgt ggggagggtt gcaggccccg agaatcacgt agggcaacat
     6061 gtccttcccg agtttcgctt tcatcgttgc ctggacgtcg atttccgtga gaacaccaaa
     6121 cccttccgtc gcaagtgctg ccttcgttgc agcaactgct tgcacgaagg aagtattcac
     6181 ctctgccttg attgcgtagt gcgccatccg gcttcacacc tttcattgtg cgggaacacg
     6241 taacaccttc ctggtcattg atactaccga tgcatcagca acgttggttc cgacgccggt
     6301 gttgcactcc cgcctgctgt ctcgtgtgac cttcgccccc gactcgcgtt cgctccggtt
     6361 cacgcgttgt ggaaagaatc accgccgcgg cgcgatgtcc cagtcccgca tccgcctcca
     6421 aagcgttatc cggcttatgc ccagttcttt tgccgccgcg gtgcggttgt aacgatgttt
     6481 ctccaacgcc attcggatcg cctgttcgtc gagaatgcag tttcgagcaa ccgcaagctc
     6541 ggagggttgc acgattgacg gaggaagatg atgcacgtca atcgttgagc cttgactacg
     6601 tataatcgcg tactccagcg cggcctgtaa ctcccggaca ttgcccggcc atcggtagga
     6661 acgaaagagc cggagcacat cgtctgtaac gccgggtgct gcgcttccgt gtctgtcagc
     6721 tattctcgaa agaagccgat gtacaagcaa gggtaggtcg tcaacccttt ccc
//
LOCUS       DAOSOO010000174         6742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11389_length_6742_cov_5.575744, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000174 DAOSOO010000000
VERSION     DAOSOO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11389_length_6742_cov_5.575744"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLT86_11170"
     CDS             <1..432
                     /locus_tag="PLT86_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37213.1"
                     /translation="RSLDIAMNIGNVDYGHVHDVLFEDIRVEMDPVYQRPRIQREDAE
                     TFPVDPHSTWLPTLMGCSITQHHEYSEGRPERGRISDITFRNIHVSSDRMPPSSFRGY
                     DAHHRVANVTIDGLYLNGIRMKTLEEAAVTIGDFAENIALK"
     gene            550..2244
                     /locus_tag="PLT86_11175"
     CDS             550..2244
                     /locus_tag="PLT86_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOT37214.1"
                     /translation="MEQETGALGIIVEPDVPVRMRDGITLRANVFRPDSSGRFPALVQ
                     RTPYGKAQGGFEDFVRAGYVVVTQDTRGRYASEGEFRVFSEENTGDAEDGYDTVEWAA
                     VQPWCDGKVGTFGASYNAWMQYQLARLRPPHLVAMSAVSIPTELTNLDWPGAFKPARR
                     VKWWVTTIAPDLRRRKGLPPPHTPAEAAKIWDELEQGRMLALLPWINIARYLPAPLAD
                     QVAGWLRHPDRRPWRFEEAHRDIEVPNLDFTGWFDHCCSISQFIGMRANARTEKARKH
                     TKVVIGPWNHCNPGRRKQADFDFGPDAEVNVQQMQIRWFDYWLKGIDNGVPREPPVRY
                     FVMGSGRWKSAETWPPPNLGRMTLYLAREGDADLPGGFGALGHEPPKNGPPDTYTHDP
                     FDPVPTLWDTAYFYNVSDRRRLDHRNDILRYCTAPLKEDVEVVGNPEVVLYAASSAVD
                     TDFFARLVDDDPGGTAMEVCYGMVRARHRNGLGKEDLLTPGEVTRFAIRMGIASCCFR
                     KGHRIRLEICSSDFPNHDRNHNTGRNDLLDAEMVIAEQKVHHSPEHPSALILPVSQ"
     gene            2402..4003
                     /locus_tag="PLT86_11180"
     CDS             2402..4003
                     /locus_tag="PLT86_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006882223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37215.1"
                     /translation="MPAKPNFLWISFEDTNPFYGCYGDPVARTPNLDRLAAGGCRWTH
                     CFSTAPVCAPARSAVITGMYPISIGTHHMRTTHMNPGTPELPTPYSAVVPHYVKCFTE
                     YLRAAGYFCTNNAKTDYQFDPPVTAWDECGTHAHWRNRPDPDQPFFAVFNPTRTHESG
                     MWPEHCPELRFDPAEMKLPPYFPDTPKVRQAMARMYTHIEESDAELGQLLAQLEEDGL
                     ADNTYVFNWSDHGPLPRGKRWPYDSGIHVPLICRGPGIEPGKVSSDLVSTIDLGPTVL
                     SLAGLEVPYHMQGQAFLGLRAKPPRDYVYVSRDRYDESYDMVRAVRDRRFKYIRNCRP
                     ELPYQLWIPYLNRHPIMQEVWRLYLAGELNETQLLMFQYPRPTEELYDTESDPYEIHN
                     LAGDAAHEGDLVRLRAALDNWLREVGDMGELSESEMVRRWYPDGVQPVTAPPTFIPIC
                     REIPGTQQAWEQCTLTAPALIQLHCATQGASIAYTFDSESTPRWLLYAEPLRIAPGDH
                     VLRCKAIRIGYRESEERRLELHVRA"
     gene            complement(4294..4500)
                     /locus_tag="PLT86_11185"
     CDS             complement(4294..4500)
                     /locus_tag="PLT86_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37216.1"
                     /translation="MAMFDWALAKARTFTVWDWGALKICLFSAGLLVAKLWPGVLGLE
                     WYWYALIFAITYVWVMVRMFAKSA"
     gene            complement(4600..5436)
                     /locus_tag="PLT86_11190"
     CDS             complement(4600..5436)
                     /locus_tag="PLT86_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT37217.1"
                     /translation="MTDPVATITTLVDNNARNGLAGEHGFALWIETDGKRILFDTGQG
                     GVLSANARALGVSLHDADFIVLSHGHYDHTGGLPRVLDEAGGSVTCCHPEVTKPRYSV
                     REGSAREIGMPEDAVRAIENLPAQRMKWADEPLFLTNRIGVTGPIPRETAFEDTGGAF
                     FLDPEGVHPDLIQDDIALWIRTAEGTVLCAGCSHAGIVNTASHVQRLTNGERIRAVIG
                     GFHLLNAGRDRMEQTVASLRRFDIDKLVPCHCTGEHAVEALRDAFGERVMPGAAGMVL
                     AF"
     gene            complement(5555..5630)
                     /locus_tag="PLT86_11195"
     tRNA            complement(5555..5630)
                     /locus_tag="PLT86_11195"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5595..5597),aa:Asn,seq:gtt)
     gene            5805..>6742
                     /locus_tag="PLT86_11200"
     CDS             5805..>6742
                     /locus_tag="PLT86_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37218.1"
                     /translation="MQSDSSLPKMLCYSIPPNEYLNTHAAEIRGLYDGFYFQCGTWDA
                     GAAANIGLPNEPPVNPEWRALARQNVAVLKHHGINENLIAVAFGSGDAWPSPETLLSD
                     AFTEKMGRHFRALGRAARECGFRGVSIDLEYPYPRYELDHPQYVYDTYTPGDLKAAAY
                     RQGRTCMAALLDEFPDAVVFLLPGGIRTRPLEVQMILGLMEEMAERDAPGGCHLASEY
                     SYRMHDPVTAASVPVFDECRMGNFVSDSTIAYWKRRGSVAPGVWAFHMAESEETDYPV
                     RPWSEEMAERRQQMRILRSLSPRYMWSYSSYAAWFAP"
BASE COUNT         1331 a         2174 c         1928 g         1309 t
ORIGIN      
        1 cgcagtctgg atatcgccat gaacatcggc aatgtggact acggccatgt tcacgatgta
       61 ctattcgaag acattcgggt ggagatggat cctgtgtacc agcggccgcg cattcagagg
      121 gaggatgcgg agacctttcc cgtggacccg cactccacat ggttgccaac gctcatgggt
      181 tgcagcatta cgcaacacca cgaatacagc gaaggcagac cggagcgcgg gcgcatttcc
      241 gacatcacgt ttcgcaacat tcacgtgtcg tccgaccgca tgccgccatc ctcgttccgg
      301 gggtacgatg cgcaccaccg ggtggcaaac gtgacgatcg atggtctgta cctaaacggt
      361 atccgcatga agacgctcga agaagctgcc gtgactatcg gggacttcgc ggaaaacatc
      421 gcgctcaagt agccgagggc aatcgcggcc ccgttcgacc ttctggctgc gaacacgcca
      481 tcagcggatt actgtcgcgg acaccttgcg ccgcctggat ccgaattagc atctggagga
      541 gaccacgcca tggagcaaga gaccggtgcg ttgggcatta tcgtcgaacc tgatgtcccc
      601 gtgcggatgc gggacggcat cacgttgcgc gccaacgtgt ttcgtccgga ttcatcaggc
      661 cgtttccccg cgcttgtgca gaggacgccg tacgggaagg cccagggcgg gttcgaggac
      721 tttgtccgtg cggggtacgt ggtcgtcacc caggataccc gcggtcgcta cgcgtctgag
      781 ggcgaattca gggtgttctc cgaggagaac acgggggacg cggaggacgg ttacgatacc
      841 gtggaatggg ctgctgttca gccctggtgc gacgggaagg tgggcacatt cggcgcatcc
      901 tacaacgcct ggatgcagta ccagcttgcc cggctccggc cgccacatct ggtggcaatg
      961 agcgccgttt ccattccgac cgaactgacc aacttggact ggcccggcgc gttcaagccg
     1021 gcgcgtcgcg tcaaatggtg ggtgaccacc atcgctcctg atctccggcg tcgaaagggc
     1081 cttcctccgc ctcacacgcc ggcggaggct gcgaagattt gggacgaact cgaacagggc
     1141 agaatgctcg ccctcttgcc ctggatcaac attgcccggt atctccccgc accgctcgcc
     1201 gaccaggtgg ccggatggtt gcggcatcct gaccgacgcc cgtggagatt cgaggaggct
     1261 cacagggaca ttgaagtgcc taacctcgac ttcaccggct ggttcgatca ctgctgcagc
     1321 ataagccagt tcatcggaat gcgggcgaat gcccgcaccg agaaggcgcg caagcataca
     1381 aaagtcgtca tcgggccctg gaatcactgc aacccgggcc gccgaaaaca agcggacttc
     1441 gatttcggcc cggatgccga agtgaatgtg caacaaatgc agatacgctg gttcgattat
     1501 tggctcaaag ggatcgataa tggtgtgccc cgggagcctc cggtgcgcta ctttgtcatg
     1561 ggatcgggaa ggtggaaatc ggccgagacg tggccaccgc cgaacctcgg ccggatgacc
     1621 ctgtaccttg cacgcgaggg agatgccgac ctacccggcg gcttcggggc cttaggacat
     1681 gaacccccga aaaacggacc gcccgacacg tacacccacg acccgtttga tcccgtcccg
     1741 actctgtggg acacggctta cttctacaac gtgtccgacc gccgccgcct tgatcaccgc
     1801 aacgacatcc tgcgttattg caccgcgccg ctcaaagagg atgtcgaggt cgtggggaac
     1861 ccggaggttg tactttacgc agcctcctcc gcggttgaca cggacttctt tgcccggctt
     1921 gtggacgatg accccggtgg aacagccatg gaggtctgtt acgggatggt tcgcgcgagg
     1981 caccgcaacg gattgggtaa ggaagacctc ctgactcccg gcgaagtgac ccggtttgcc
     2041 attcggatgg ggattgcgtc atgctgtttc cgcaagggcc accgcattcg actcgaaatc
     2101 tgcagcagcg actttcccaa tcacgatcgt aaccacaaca ctggcagaaa tgacctcctg
     2161 gatgcagaga tggtgatcgc cgaacagaag gttcaccaca gcccggaaca cccttcggcg
     2221 ctcattcttc cggtgagcca ataaatggat tccttgacac aggggcagga acgctggtcc
     2281 ggtccaccag gcccacaccg tccaccattc tcttcgtgtc ttcgcgtgag acatttccga
     2341 acggacctca gaccgctgga ttccgcacct gtctcttgcg gacaatagaa ggaactcacc
     2401 catgcccgcc aaacccaact tcctctggat atccttcgag gataccaatc cgttctacgg
     2461 ctgttacggc gacccggtag cccgcacgcc gaacctcgac cggctggcgg cgggcggctg
     2521 ccggtggacg cactgctttt ccacggctcc ggtgtgcgcc cctgcgcggt cggccgtgat
     2581 caccggcatg tatccgatct ccatcggcac gcaccacatg cgcacgacgc acatgaaccc
     2641 gggaacgcct gagttgccca cgccctattc cgccgtggtg ccgcattacg tgaagtgctt
     2701 caccgaatac ctgcgtgcgg cgggctactt ctgcaccaac aacgcgaaaa ccgactacca
     2761 gtttgatcca cccgtcaccg cttgggatga atgtggcact cacgcccact ggcgcaatcg
     2821 ccccgacccc gatcagccgt ttttcgcggt gttcaacccg acgcgcaccc atgaaagcgg
     2881 catgtggccg gaacattgcc cggagctcag atttgacccc gcggagatga aactgccgcc
     2941 ctactttccc gatacgccga aggtacgcca ggctatggcc cggatgtaca cgcacatcga
     3001 ggagagcgac gcagaactcg ggcagcttct cgcgcaactg gaagaggacg gactggcgga
     3061 caacacgtat gtcttcaact ggagcgacca tgggccgctg ccgaggggaa aacgctggcc
     3121 gtacgactcc ggcatccatg tgccgcttat ctgccgggga cccggcatcg aaccgggtaa
     3181 ggtcagcagc gacctcgtca gcaccatcga ccttggaccg accgtcctct cccttgcggg
     3241 gttagaagtt ccttaccaca tgcaggggca ggcgtttctg gggctaaggg cgaaaccgcc
     3301 gcgcgattac gtctatgtca gccgggacag gtacgatgaa tcatacgaca tggtgcgcgc
     3361 cgtgcgggat cgccgcttca agtacatccg caactgccgc ccggagcttc cgtaccagct
     3421 ctggattccg tacctgaacc gccacccgat catgcaggag gtgtggcgtc tctacctcgc
     3481 gggcgaattg aatgagacgc aattactgat gttccagtat ccccgcccga cagaagagtt
     3541 gtacgacacg gaatccgatc cgtatgaaat tcataacctt gccggcgacg cggcccacga
     3601 gggggacctt gtccggctcc gcgcggcgtt ggacaactgg ctgcgcgaag tcggcgatat
     3661 gggggagctt tctgaaagcg agatggtgcg gcggtggtac ccggacggcg tccaaccggt
     3721 aaccgcgccg cccacgttca ttcctatctg ccgggagatt ccgggaacgc agcaggcgtg
     3781 ggagcagtgc acgctcaccg ccccggcgct gatccagttg cattgcgcaa cacagggcgc
     3841 gtcaatcgca tacaccttcg attcggaaag cacgccccgc tggttgctct atgcggaacc
     3901 cttgcggatt gcgccgggag accacgttct gcgctgcaag gcgatcagga ttggataccg
     3961 ggaaagtgag gaaaggcggt tggagcttca cgtgcgcgcg tagcgagtag taaaccctga
     4021 cagaaaagat cgagccgaaa gagagcaggt tcctccacca agcgaacaga gccctcctac
     4081 gtcttccgat gcccttcgtg ttcttcgtgt cttcgtgtga agcaggtgac ctttgccctg
     4141 ggcgagcccc tccgtgttct tcgcgccttc gtgtgatcca ttcagtcccg tccgggttcc
     4201 cgcggcccgt tgtcccgcaa cgccctcccg aagtggaagg tcattcccat tttccttcgc
     4261 gttcttcgcg tcttcgtgtg aggcgagccg tgttcaggcg cttttcgcga acatcctcac
     4321 catcacccag acgtacgtaa tcgcgaaaat gagcgcgtac cagtaccatt caagccccag
     4381 cacgcccggc cagagcttcg ctacgagcaa ccccgcagag aagagacaga tcttcaacgc
     4441 gccccagtcc cacacggtga acgtcctcgc cttcgccagt gcccagtcaa acattgccat
     4501 tgtgcccctc ctgcccattt acccccgggt tcttcccttc gcgttcttcg tgtcttcgcg
     4561 tgagacagtt tcctgtcgcc cctctttttg gcaagcgttt cagaaagcca acaccatccc
     4621 cgccgcaccc ggcatcaccc gttcaccaaa cgcgtctcga agggcttcga ccgcgtgttc
     4681 gccggtgcag tggcagggaa ccagcttatc aatatcaaac cgtcgcaacg acgcaaccgt
     4741 ctgctccata cgatccctcc ctgcgttcag caggtggaat ccgccgatca ccgcgcgaat
     4801 cctttccccg tttgtcaatc gctggacatg actcgctgtg ttcacaatcc ccgcgtgcga
     4861 gcaaccggcg cagagcaccg tcccttccgc ggtccgaatc cacaaagcga tatcgtcctg
     4921 aatgaggtcc gggtgcactc cttcggggtc gaggaaaaac gctccgcccg tgtcttcgaa
     4981 cgccgtttcc cgtggaatgg ggccggtcac gcctatacga ttcgtcagga acagcggttc
     5041 gtcagcccat ttcatgcgct gcgccggcag gttttctatc gcgcgcaccg cgtcttccgg
     5101 catcccgatc tctctggccg acccttctct aaccgaatac cggggcttcg tcacttccgg
     5161 gtggcagcag gtgacactcc ctccggcctc gtcaagaacc ctcggcagtc cacccgtgtg
     5221 gtcgtaatgc ccatggctca gcacgatgaa gtccgcatcg tgaaggctga ctccgagtgc
     5281 ccgtgcattc gcgctgagaa cgcccccttg ccctgtgtcg aacaggatgc gtttaccgtc
     5341 ggtttctatc cacagcgcga agccatgctc gccagctagc ccgtttctgg cgttgttgtc
     5401 gacaagggtg gtaatagtgg cgacgggatc tgtcacgagg ttgtggtcct tccaaaacaa
     5461 aaaaaaagca acaggggaag cagccgcttc ggaccgcttc ccctgctctc aggaacaaac
     5521 cgctgagcct tgcaaggtcc gcggttatgg atgatggctc gggcgacagg acttgaacct
     5581 gtaacctagt ggttaacagc cacccgctct gccgattgag ctacgcccga tcacagactg
     5641 acaggaaatg ttgaataatg aattatactt ccttccggtc gtgccgtcaa ttcctctccg
     5701 gggagccgaa gggctccacg ccgccttgct cctccatctc ggcccggcat aactttttct
     5761 gtcgccgcaa aacgaccgtc aatcccaccc gcaggagtca tccggtgcag tcagattcat
     5821 ccctcccgaa aatgctctgc tactcgattc caccaaacga atacctcaac acccatgccg
     5881 cagaaatccg ggggctctac gacggctttt acttccagtg cggaacgtgg gatgccggcg
     5941 cggcagcgaa tatcggtctc ccaaacgagc cccctgtcaa tccggaatgg cgtgcgctgg
     6001 cccgtcagaa cgtcgccgtg ttgaaacacc acggcatcaa cgagaacctc atcgccgtgg
     6061 cattcgggtc aggtgacgca tggccgtcgc cggagacgct cctttccgac gcgttcaccg
     6121 aaaagatggg ccggcacttc cgagcgctcg gcagggcagc acgggaatgc ggattccgag
     6181 gcgtgtccat tgatctcgaa tacccgtacc cgcgttatga gcttgatcac ccgcagtacg
     6241 tctacgacac ttacacgccc ggggacctga aggcggccgc ctaccgccag ggacgcacct
     6301 gcatggcggc gctgctcgac gagttccctg acgcggtcgt ctttcttctt cccggcggca
     6361 tccggacgcg tcctctggaa gttcagatga ttctgggcct catggaagaa atggcggagc
     6421 gcgacgcgcc ggggggctgc cacctcgcgt ccgaatactc ctaccgcatg cacgatcccg
     6481 tcaccgcggc ctcagttccc gtgtttgatg agtgccgcat gggcaatttc gtgtccgatt
     6541 ccaccatcgc ctactggaag cgccgcggct ccgttgcgcc gggcgtctgg gcattccaca
     6601 tggccgagag cgaggaaacc gactaccccg tcagaccctg gtcggaggag atggccgaac
     6661 gccggcagca gatgcgcatc cttcgctccc tgtccccgcg gtacatgtgg tcgtactcat
     6721 cttacgcggc gtggtttgcc cc
//
LOCUS       DAOSOO010000175         6636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11642_length_6636_cov_5.945145, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000175 DAOSOO010000000
VERSION     DAOSOO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11642_length_6636_cov_5.945145"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLT86_11205"
     CDS             <1..774
                     /locus_tag="PLT86_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37219.1"
                     /translation="LLYDMEIPGVFSTPLGRRTLEYMDYFHDKAAREEFDGRPVAPPT
                     YAGFFTQGPFELAGHLRGTTELCVDMYDDPTYFHDLMDFCTRAIILRVKTIFGDLLGV
                     PMPFEHFAYTDDSIAMLSAEAFQKNVWPYHKRMFDELATPTCTHFLHLCGTVQQHIPF
                     LASTGLINYLGTSYPVDIPRARREAGDDLWLDTAIHADIVLRGSAEAIDAAVAAKLTP
                     EAKGKGRLVFMGGNYIAPGTTLDKFLMLYESVKRHGVYR"
     gene            788..1873
                     /locus_tag="PLT86_11210"
     CDS             788..1873
                     /locus_tag="PLT86_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOT37220.1"
                     /translation="MAGFNGLGMNLGNLSRLSTAKTRSISAENFTGEKGKGGMAKEGT
                     GANAARDLGQGWKISPCVRVKAGETFEMANIEGPGAIQHIWLTPSGHWRFQILRIYWD
                     NAKTPSVECPVGDFFACGWNQYAQVSSLPVCVNPGRALNCYWEMPFRKRCRMTMTNIG
                     KEDVTLFYQVDYTLTDVPDDCAYFHAQFRRTNPLPYKEVYTIVDGIKGRGQYVGTYMA
                     WGVNNCGWWGEGEIKFYMDGDKKFPTICGTGTEDYFCGSYNFDVGKENGGYREFTTPY
                     AGLPQVIRPDGLYSSQQRFGLYRWHIMDPIRFEKDLRVTIQSLGWRSGGRYLPNQDDM
                     ASVAFWYQTLPAAPFPKLPDADYLEVI"
     assembly_gap    2010..2109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2203..4191)
                     /gene="uvrB"
                     /locus_tag="PLT86_11215"
     CDS             complement(2203..4191)
                     /gene="uvrB"
                     /locus_tag="PLT86_11215"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT37221.1"
                     /translation="MPEFQLESDYTPQGDQPRAIAELTEGILRGDRFQTLLGVTGSGK
                     TYTMASVIANVQKPTLVVSHNKTLAAQLYGEFRSFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIEKDASINEEIDRLRLKATSALFERRDVVVVASVSCIYGLGSPEEFRRQLVVL
                     ERGKRVNRDAMLRRLVDIHYSRNDLAPERGTFRARGDIVEVRPADEDYVIRVELFDDE
                     VESLSITDPLTGHVLEKRDRVAIYPAKHFVTTVPQIEAACQSIMVELTQRLDELRREN
                     KLVEAQRLESRTLFDIEMLREVGYCTGIENYSRHLSGRQPGERPAVLLDYFPEDFLMI
                     VDESHVTIPQFRGMYAGDRSRKQMLVEYGFRLPSALDNRPLFFEEFEALIPQAVFVSA
                     TPADYELEKSGGVIVEQVIRPTGLVDPEIIVKPVRGQVDDLLEEVRKRVAAGERTLVA
                     TLTKRMAEDLTSYLEEMGVRVRYMHHEVDAITRVETLRDLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLIQTAGRAARHVNGQVIFYADVVTDSMRRAISET
                     NRRRELQLAFNQEHGITPRSIVKSLDQVMAQTAVADSRPRIEPKVHVPVVRGKDLEAT
                     IRELERLMLEAAKDLEFEKAAAIRDEIADLKKQAGNGK"
     gene            4387..5907
                     /locus_tag="PLT86_11220"
     CDS             4387..5907
                     /locus_tag="PLT86_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37222.1"
                     /translation="MKQLWNMTGPVLGLAALLLLGVHAAQAEDVTAQASTSNQSISLG
                     STTIDGEQWQRLAFRREMPFGKLAIAMDIEFFVNSEGGLSSRGWQFGNFNQVMSTLYR
                     KIYYVRYGQPRDPVYVRLMGLDDVTLGYGFLMSHYRNTLDYPGVKNLGVHFELNPAGG
                     ISVQGVLNNLLDITNGGPVVGARVAKGLGPFEVGASLVYDVDQYGGLADNNDDGLPDA
                     LEPRVGADGVNYSQIRNPSDQDQLNSILVANGQPPINWARYARQDSLYLKGHRETDSY
                     GMVGADVAMKLIDRPSLALITYGQMGISLDDNDRSGRATGWGFGAPGLMAVFGPVRAR
                     IEYRHLRDEFQPEYFNSWYDHTRATVSADSGVITLKDASLNALAGQTLNGVFGDASVR
                     VPLLGEASAQYQYLHGPTNQQRLSALAGVDQGLLQMVPKLSRIEAFYAKDNIGLYGDG
                     FFQKTVDMSYGYRVGFTVAPSVEMIWSTRWLFRPVGSDPLVVEPQRQITFATVFNF"
     gene            complement(6019..>6636)
                     /locus_tag="PLT86_11225"
     CDS             complement(6019..>6636)
                     /locus_tag="PLT86_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT37223.1"
                     /translation="LKRLFERDAPRVFVALGNRALMEQNGIAGSHEMDWWQDTLITPA
                     VRLTFVPAQHFSARGFGDRNETLWGGYVLEGEAGVVYFAGDTGWGPQFDQVRDRFGAI
                     RLAILPIGAFRPQWFMAAVHISPEEAVRAHRLLGAQTSIAVHFGTFRLGDDGQNEPVE
                     ELANARELHDVSPESFWVLGFGEGRMVPPVEVDTLRSAEVVEQRR"
BASE COUNT         1281 a         2015 c         2004 g         1236 t
ORIGIN      
        1 ctgctttacg acatggaaat cccgggggtt ttcagcacgc ccctcggcag gcgcacgctg
       61 gagtacatgg actatttcca cgacaaagca gcgagggagg agtttgacgg ccgcccggtt
      121 gctccaccca cctacgccgg attcttcaca cagggcccgt ttgagcttgc cggccatctc
      181 cgtggcacca ccgagctctg cgtggacatg tacgacgatc ccacctactt ccacgacctg
      241 atggatttct gcactcgggc gatcatcctg cgcgtcaaaa ctattttcgg cgatctgctg
      301 ggagttccca tgccattcga gcacttcgcg tacaccgacg acagcatcgc catgttgagc
      361 gcggaggcat tccagaaaaa cgtctggccc tatcacaagc gcatgtttga cgaactggcg
      421 acacccacgt gcacgcattt cctgcacctg tgcggaaccg tgcagcaaca catccccttc
      481 ctcgcaagta cggggcttat caactacctc ggcaccagct acccggtgga cataccccgc
      541 gcccggcgag aggcgggcga cgacctctgg ctcgacaccg cgatacatgc cgacatcgtc
      601 ctgcgcggct ccgctgaggc catagatgca gccgttgcgg cgaaactcac gcctgaggcg
      661 aaagggaagg gccggctggt gttcatgggc ggcaactaca tcgcaccggg aacgacactt
      721 gacaagttcc tgatgctcta cgaatcggtg aaacgacacg gggtctaccg ctgacaagga
      781 ggaacaaatg gcgggattca acggacttgg tatgaacctc ggtaaccttt cgcgcctctc
      841 cacggcaaaa acacgttcga taagcgcgga gaactttacg ggcgaaaagg gcaagggcgg
      901 gatggcgaag gagggtaccg gcgccaatgc ggcacgggac ctcggtcagg gctggaaaat
      961 ctccccgtgc gtccgcgtga aggcgggcga gacgttcgaa atggcgaaca ttgaaggccc
     1021 cggcgcaatt cagcacatct ggctcacccc gagcggacac tggcggttcc agattctccg
     1081 gatctactgg gataacgcaa aaacgccgtc ggtggagtgc ccggtgggtg acttcttcgc
     1141 gtgcggctgg aaccagtacg cgcaggtctc ttccctgccg gtgtgtgtga atccgggtcg
     1201 cgcgctcaac tgctactggg agatgccttt ccgcaagcgc tgccgtatga caatgactaa
     1261 catcggcaag gaagacgtca ccctgtttta tcaagttgat tacacgctca ccgacgtgcc
     1321 ggacgattgc gcctacttcc acgcacagtt ccgccgcacg aatccactgc cgtacaaaga
     1381 ggtctacacc atcgtggacg gaatcaaagg gcgcgggcag tatgtcggca cgtacatggc
     1441 gtggggagtg aacaactgcg gctggtgggg cgagggcgag atcaagttct atatggacgg
     1501 tgataagaag tttcctacca tctgcggcac cggcaccgag gattatttct gcggatcata
     1561 caatttcgat gtcggcaagg agaacggcgg ctaccgcgag ttcaccacgc cgtacgccgg
     1621 cctcccgcag gtgatccgtc cggacgggct ctattcctcg cagcaaaggt tcggcctcta
     1681 ccgctggcac atcatggacc cgatccgctt cgagaaggac ctccgggtga cgattcaatc
     1741 ccttggctgg cgttccggcg ggcgctacct gccgaatcag gacgacatgg catcggtggc
     1801 attctggtac cagaccctgc ccgccgcccc gttcccgaag ctgccggatg ccgattatct
     1861 ggaagtgatc tgatcgcgga ggggaaaagg gacgcgacga agcgcgtccc tccatagcga
     1921 gcgccgcagg aggaggacac gcacacagac tggcggggcc cgctctgtcg ggcccttgtt
     1981 ttgttcctca ttcaccccgt tcttcccgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnna aggcacagcg gtggtctggg aacaaaccgc cctagcatgt gcattccctg
     2161 cggcagggcc acggttgccg tgcgcccttc cacaggcggg cgtcacttcc cgtttccggc
     2221 ctgcttcttc aaatcggcga tttcgtcccg gatggcggcg gctttctcga attcgaggtc
     2281 cttggcggct tccagcatca gccgttccag ttcgcggatg gtggcctcca gatccttccc
     2341 cctcaccaca ggcacgtgca ccttcggctc gatcctgggg cgtgagtccg ccacggctgt
     2401 ctgcgccatc acctgatcca gcgacttgac gatggagcgc ggggtgatgc cgtgttcctg
     2461 gttgaacgcg agctggagtt cgcgccgccg gttggtttcg ctgatggccc ggcgcatgga
     2521 atcggtgacc acgtcagcgt agaagatcac ctgcccgttc acgtggcgcg cggcgcggcc
     2581 ggcggtctgg atgagcgagc gttcggagcg gagaaagccc tccttgtccg catcgaggat
     2641 ggcaacgagc gatacctccg gcaggtcgag cccttcgcgc aacaggttga tgcccaccag
     2701 cacatcgaat tcgccaaggc gaagatcgcg aagagtttcc acccgggtga tggcgtccac
     2761 ttcatggtgc atgtaccgca cgcgcacgcc catctcttcg aggtaactcg tcaggtcctc
     2821 cgccatgcgt ttcgtcagcg ttgcaaccag agtccgttcg ccggcagcga ctctcttccg
     2881 cacctcttcg aggaggtcgt ccacctgccc gcgaaccggt ttcacgatga tctccgggtc
     2941 cacaagcccg gtgggccgga taacctgctc cacgattact ccgccgctct tttccagctc
     3001 gtaatcggcg ggcgtggcgc tgacgaagac cgcctgcgga ataagcgctt cgaactcctc
     3061 gaagaacagc gggcgattat ccagcgccga ggggaggcgg aagccgtact ccacaagcat
     3121 ctgctttcgc gaacggtcgc cggcgtacat cccgcggaac tggggtatcg tgacgtgcga
     3181 ttcatccacg atcatgagga agtcctcggg gaagtagtcc agcagcacgg cggggcgctc
     3241 gcccggttgc cgtccggaga ggtgacgtga gtagttctcg atgccggtgc agtagcccac
     3301 ctcccggagc atttcgatat cgaacagggt gcggctttcc agccgctggg cttcgacgag
     3361 tttgttctcc ctgcggagtt cgtcgagccg ctgcgtgagt tccaccatga tgctctggca
     3421 ggccgcttca atctgaggaa cggtggtaac gaaatgcttc gcgggataaa tcgccacgcg
     3481 atcgcgtttt tccagaacgt ggccggtaag cgggtcggtg atggagagcg actctacctc
     3541 gtcgtcgaac agctcgacgc ggatcacgta gtcctcatcc gcgggccgaa cctccacaat
     3601 gtctcccctg gcgcggaacg tgccacgctc gggagcaagg tcgttgcggc tgtaatgaat
     3661 gtccaccagc cggcgcagca tcgcatcgcg gttgacccgt ttcccgcgtt ccagcacgac
     3721 aagctggcgg cgaaactctt ccggcgaccc caacccgtag atgcacgaca ccgacgccac
     3781 aacaaccacg tcccgccgct cgaacaacgc gctggttgcc ttcaggcgca ggcggtctat
     3841 ctcttcgttg atcgatgcat ctttttcgat atacgtatcg ctctgcggga tatacgcttc
     3901 cggctggtag taatcgtagt agctgatgaa atactccacc gcattgtggg gaaaaaacga
     3961 ccggaactcc ccgtagagct gcgccgcgag ggttttgttg tgcgaaacca ccagcgtcgg
     4021 cttctggaca ttcgcgatta ccgacgccat ggtataggtt ttgccagacc ctgttacacc
     4081 aaggagcgtc tggaagcgat cgccccgcag gatgccctcc gtcagttcgg cgatggcgcg
     4141 aggctggtcg ccctgagggg tgtagtcact ttcgagttga aattccggca taggagagct
     4201 cgttcaccag cgtagaagtc acaacgcaga taccgtcttc ctagagtata cgaacggcgg
     4261 tgcttcgtgg tgcccgcgaa accggggtgg ttcgcacccg aaggaaattg cgcgtatcat
     4321 tctcggtacg acaatccatt cgcgcgaacg cattaccccg gagcgacaca ggaaagggtg
     4381 tgcacgatga aacaactttg gaacatgaca ggaccagtgc tcgggctggc ggcgttgctg
     4441 ttgctgggcg tccatgcggc gcaggccgag gacgtgactg cccaggcgag tacctccaat
     4501 caatccatct ccctcggctc gaccacgatt gacggcgaac aatggcagcg ccttgcgttc
     4561 cgtcgggaaa tgccgttcgg aaaactcgcc atcgccatgg atatcgagtt ttttgtgaac
     4621 agcgaaggcg gcctgagcag ccggggatgg cagttcggca acttcaatca ggtgatgagc
     4681 acgttgtatc gaaaaatata ctacgtgcgg tacggtcaac cccgggaccc ggtctacgtt
     4741 cggctgatgg ggctggacga tgtgacgctg gggtatggct tcctcatgtc gcactaccgg
     4801 aacacgctgg attaccccgg tgtgaaaaac ctcggtgttc acttcgaatt gaaccccgcg
     4861 ggcggcatct ccgttcaggg cgttctgaac aacctgcttg acatcacgaa cggcgggccg
     4921 gtcgtcggtg cccgggtggc gaagggactg gggccgtttg aagtcggcgc ctcgctggta
     4981 tacgacgtgg accagtacgg cggcctcgcg gacaacaatg acgacggatt gcccgatgcg
     5041 ctggaaccac gcgtgggtgc cgacggggtg aactacagcc agatccgaaa tccttcggat
     5101 caggaccaac tcaacagcat cctggtggca aacggacaac cgcccatcaa ctgggcgaga
     5161 tacgcgcgac aggatagcct ctatctcaaa ggccaccgcg aaacggacag ctacggcatg
     5221 gtgggcgccg acgtcgccat gaaactgatt gacaggccga gccttgcgct catcacctac
     5281 gggcagatgg gtatcagcct cgacgacaac gaccggagcg gccgcgccac gggctgggga
     5341 ttcggtgccc cgggtctgat ggccgtattc gggccggtgc gggcacggat agagtaccgt
     5401 cacctgcggg atgagttcca gccggagtat ttcaactcgt ggtatgacca cacccgggcc
     5461 acggtgagcg cagattcagg agtgatcacg ctgaaggacg cctcgctgaa tgccctcgcg
     5521 ggccagacac tcaatggcgt gtttggcgac gcctcggttc gcgtgccgct cctcggggaa
     5581 gcaagtgccc agtaccagta cctccacgga ccgacaaatc aacagcggct gtccgcgctg
     5641 gcgggggttg accagggcct gttgcagatg gtgccgaagc tcagcaggat tgaggcattc
     5701 tacgcgaagg acaacatcgg gctgtacggg gacgggttct tccagaagac ggttgatatg
     5761 tcgtacggct accgggtcgg cttcacggtg gcgccgagtg tcgaaatgat ctggtctacc
     5821 cgctggttgt tccgtcccgt cggctcggat ccgctggtgg tggagccgca gcggcagatc
     5881 acgttcgcca cggtgttcaa cttctgacgc aagacgggga attgggtgta gcgggaggtg
     5941 ccggggtcgg tgcctcccgt ttttctgttc cggcgcaatc cgccgcgcgg gaatgttgcg
     6001 gcgcgcagcc ggacgctctc agcgccgctg ttcgaccact tccgcacttc tcagcgtatc
     6061 cacctcaacc ggaggcacca tgcggccttc gccgaagccg agcacccaga acgactccgg
     6121 cgaaacatcg tgcagttcgc gggcgttggc cagctcttcc acgggttcgt tctggccatc
     6181 atcccccagc cggaacgtgc cgaaatgcac agcaatgctg gtttgcgcgc cgaggagccg
     6241 gtgagcccgc acggcttctt ccggggagat gtgcaccgcc gccatgaacc attgcgggcg
     6301 gaacgcgccg ataggcagaa tggccagccg gatggcgccg aagcggtcgc gcacctggtc
     6361 gaactgcggc ccccagccag tatcgcccgc gaagtagacg acacccgcct cgccttcaag
     6421 gacgtacccg ccccacagcg tctcgttgcg gtccccgaac ccgcgcgcgg agaaatgctg
     6481 cgccggaaca aacgtcagcc gcacagcagg cgtgatcaga gtatcctgcc accagtccat
     6541 ctcatgcgaa ccggcgatgc cgttctgctc catgagcgct ctgttgccca gtgccacaaa
     6601 aacgcgagga gcgtcgcgct cgaagagccg tttgag
//
LOCUS       DAOSOO010000176         6618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11693_length_6618_cov_5.459241, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000176 DAOSOO010000000
VERSION     DAOSOO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11693_length_6618_cov_5.459241"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLT86_11230"
     CDS             <1..266
                     /locus_tag="PLT86_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37224.1"
                     /translation="SGPVIGGKPRQDAGRTVRPVMKALDMVADALAEGRDIPAEAIAL
                     MARPLMPHWVYRSVATLGLWWTARKNGVSGKLRARPFTSPAEP"
     gene            266..991
                     /locus_tag="PLT86_11235"
     CDS             266..991
                     /locus_tag="PLT86_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOT37225.1"
                     /translation="MANTGDTIQPGDIRYAEDSICPAGCLTPDEELIRTVANRVLAAL
                     QNTADPESPKLVPVGISARHCHVTEEVLQILYGRGAKLHPYRELRQPGFYAARETVTV
                     LGPRMRAIENVRILGPTRTYTQVELSRTDGFLLGLSLPIRDSGDLEGTPGVTLVGPAG
                     SITLPNGAMRAGRHVHMSPEVAAAWGLTDRQIVRAEFEGEKGVVFSNVLIRVGKGQVL
                     EMHLDTDDGNAADLVTGDMVRLF"
     gene            1193..2305
                     /gene="gmd"
                     /locus_tag="PLT86_11240"
     CDS             1193..2305
                     /gene="gmd"
                     /locus_tag="PLT86_11240"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT37226.1"
                     /translation="MRKKALITGITGQDGAYLSRLLLEKGYEVHGIKRRSSQFNTARI
                     DDLYQDPHESDRRLFLHYGDVTDALHMFQIVKNVQPDEIYNLAAQSHVAVSFQTPEYT
                     ANVDAIGPLRILEAIRNADLVGKTRFYQASTSEMFGMVRETPQTEKTPFYPRSPYAAA
                     KLYAYWVTVNYREAYGVFACNGILFNHESPMRGETFVTRKITRAVARIKHGLQKCLYL
                     GNLDALRDWGHARDFVEAMWLMLQQERPDDFVIATGEQHSVREFVVLAFREVGIEIEW
                     KGSGDNEHAIATGPVCEGRSPDDNIVVAVDPRYRRPSEVETLLGDATKARTQLGWVPR
                     TTFSELVKEMVAGDLEQVERDLLVRRHGYSTPDYHE"
     gene            2378..3358
                     /locus_tag="PLT86_11245"
     CDS             2378..3358
                     /locus_tag="PLT86_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOT37227.1"
                     /translation="MPTDAKIYVPGHRGLVGSAIVRRLESLGYRNLILRTRAELDLLD
                     ARAVASLFAEEKPEFVFVCAAKVGGIMANNTYPAEFFYENVAIETAIIHEAWRNNVRR
                     LLFLGSSCIYPRDCPQPIREEYLMTGPLEPTNRAYAVAKIAGIEMCRAYNQQYGTRYL
                     AAMPTNLYGQGDNYDIQTSHVLPALIRKMHEAKIRGDASVTLWGTGRPYREFLYSDDL
                     ADACVFLLNLDDERFGMLLGDGSSNPLINVGCGEDLTIADLAGLVKDVVGFEGTIVWD
                     TTKPDGTPRKLLDVSKLNGLGWRPSIPLRDGITRAYRQFLEHENNARGRS"
     gene            3403..4032
                     /locus_tag="PLT86_11250"
     CDS             3403..4032
                     /locus_tag="PLT86_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HOT37228.1"
                     /translation="MDWYGIIKTTIGILAIVNPLGAVGTFLSATENLPAAARRKTADT
                     AAVTVTVTLLVAAWVGETILHFFGISIPAFRVGGGILVLSMAISMLHARPSPAKQVPE
                     EAADAVERQAVGVVPLGIPLMSGPGAIALVIVESHRAPTVPERLLVSLCIVLVAVVSW
                     TILRLAGLLAKRLTRTHINISTRLMGLILAAIAVEFIASGALVLFPGLR"
     gene            complement(4038..4772)
                     /locus_tag="PLT86_11255"
     CDS             complement(4038..4772)
                     /locus_tag="PLT86_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528096.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT37229.1"
                     /translation="MSEKFPQQFAGMLALALALVIASFVGYAAVNRAKRAGDTITVTG
                     SAKKAITSDRVVWRGSVSAQKPSMQEAYGDVKRYSERVRAYFREQGIPDSAVTFQAMQ
                     TNQIQEVLSGGHMTGRIIAYQIFQGFEIQSEDVDRITRLSADAPDLMKEGVPISSNPP
                     EFVYSNLASVRVEMLAEAAQDARKRAETIAQAVGSRIGAVRSARMGVFQITPRNSTMV
                     SDYGINDTSSREKDVTAVVSVTFAVE"
     gene            complement(4924..>6618)
                     /locus_tag="PLT86_11260"
     CDS             complement(4924..>6618)
                     /locus_tag="PLT86_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT37230.1"
                     /translation="SVDTAADGLRVVQGEFISTTDHRHNNVGAYSTFAEVKLRNRRSE
                     WGLRTLEAMATLAMRDGGIYPTKHAARAWRLTLFNQMHDIFPGTAIFEAYEEAYKRYD
                     EVDSICSIGTNAVANVLASTINTKGDGIPVVLFNTLGWDRNDPAEILLTEPHSYCDGF
                     EVRDGNGRPVPAQTLSTDIGTFDKTNKNFRLLVQPDGIPAMGYKTVWLHPAHRDERVF
                     SSMVGADRLTLDNDFVRVTINPRTGWVSQLFDKRLNRNVLPDGKEACAIEGQGDVGNP
                     WHLCPEGPVRWMNQTVRTEVVEDGDVRAAIRVTTTWHRSTFVQEFRITRTSPRVEVHT
                     TIDCHEANFTMKALIPVALPADAPWTCEVPWGAVQRPIPDNDRATQTWMDVSNDKWGL
                     SVLNNGRYGHSRRADGTMTVTLLRTTVAHKSQDQSDEGRHEFTYALLPHLGNWTEAAT
                     VREAHMLNAPIITTREIPHEGARPDTHSMIRIGAPNIVLAALKKAEDNDDWVLHLYET
                     TGAATDVTITFDRPVVEAYDTDLMEWNRGVRLNMEGGTVRRSFRPWEIAGIRIRLG"
BASE COUNT         1264 a         2045 c         1993 g         1316 t
ORIGIN      
        1 ggagtggccc cgtgatcggc gggaagccgc gtcaggacgc aggcagaacc gtgcggccgg
       61 tcatgaaagc gctcgacatg gtagcggatg cgctggccga aggccgggat atccctgctg
      121 aggcaatcgc gttgatggca aggccgctga tgcctcactg ggtgtatcga agcgtcgcga
      181 cgctcggatt atggtggacg gcccgcaaga acggtgtatc cgggaagctg cgcgcgcgtc
      241 catttacctc acccgcggaa ccctgatggc gaatacaggc gacactatcc agcccggtga
      301 catccgttac gcggaggatt ccatttgccc ggcagggtgc ctcacccccg atgaggaact
      361 catccggacc gtcgccaatc gagttctcgc cgcccttcag aacaccgccg acccggaatc
      421 ccccaaactg gtccctgtcg gcatttccgc acgccactgc cacgtcaccg aggaggtgct
      481 gcagatcctc tacggccggg gcgcgaagct ccatccctac cgcgagttga ggcaacccgg
      541 gttctacgcc gcgcgggaaa ccgttacggt gctggggccg aggatgagag cgatagaaaa
      601 cgtgaggatt ctcgggccaa cccggacata cacgcaggtg gagctttccc gcaccgacgg
      661 ttttctgctc gggctctccc ttccgatccg tgacagcggc gacctggagg gtacccccgg
      721 tgtgacactg gtgggcccgg ccggcagtat cacactgccc aacggcgcaa tgagagcggg
      781 acggcacgtg cacatgtctc cggaagttgc cgcggcatgg gggttgaccg accggcagat
      841 cgtccgcgcc gagttcgagg gggagaaggg cgttgtgttc tccaacgtcc tcatccgggt
      901 aggcaagggg caggtgcttg aaatgcacct tgataccgac gatggcaacg cggccgacct
      961 tgttacgggt gacatggtgc gtctgttctg agacatccgc ccgccttatt cgttcaactg
     1021 cgatcgcgag tgtgccggga acctttccgg gcgcaccttc agatttacgt tccgggtgca
     1081 ggctgcggcc cctgttttcg ttccgtggcc gtacccgagc cagtcgtttc cagacaaggc
     1141 gtcgcgcgcc ggtgcgtgga taaccgaacg cccgacgcgc caggagtgta acatgcggaa
     1201 gaaagctctc ataaccggga taacaggaca ggacggcgca tacctgagcc ggctgttgct
     1261 ggaaaagggc tacgaagtcc acggaatcaa gcgccgctct tcgcagttca acaccgcaag
     1321 aattgacgat ctctatcaag atccgcacga gagcgacagg aggttgttcc ttcactacgg
     1381 tgacgtaacg gacgcgctcc acatgttcca gatcgtgaaa aacgtccagc cggacgagat
     1441 ctacaacctt gcggcccaga gccacgtggc cgtgtcgttc cagactccgg agtacaccgc
     1501 caacgtagat gcaatcggcc ccctccgcat actggaagca atcagaaacg ctgatctggt
     1561 gggcaagaca cgcttctatc aggcatccac gtctgaaatg ttcggcatgg tgcgggaaac
     1621 ccctcagacc gagaaaacgc cgttctatcc gcgctcgccg tacgccgcgg cgaagttgta
     1681 cgcgtactgg gtcacggtga attaccgtga ggcgtacggc gtgtttgcgt gtaatggcat
     1741 cctgttcaac catgaatccc ccatgcgcgg ggaaacgttt gtgacgcgca aaatcacgag
     1801 ggccgtcgca cgcataaaac acgggcttca gaaatgcctt tacctcggca atctggacgc
     1861 attgcgggac tggggccacg cgcgtgattt cgtcgaggca atgtggctga tgctgcaaca
     1921 ggagcgcccc gacgatttcg tgattgccac gggagaacag cactccgttc gcgagtttgt
     1981 cgttctggcg ttccgcgagg tcggcattga aatcgaatgg aagggcagtg gagacaatga
     2041 gcacgccatt gccaccggtc cggtgtgcga agggcgttct cctgatgaca acatcgttgt
     2101 tgccgtggat ccccgttacc gcaggccctc cgaagtggag actcttctcg gcgacgcaac
     2161 gaaagccaga acgcaacttg ggtgggtgcc gcgcaccacg ttcagcgaac tggtgaagga
     2221 aatggtggcg ggcgaccttg aacaggtcga gcgtgacctt ctggtaaggc gccacgggta
     2281 ctccacgccg gattaccacg aatgagccgc acgaagctcg atcacgtgca gaggaaggag
     2341 gcttgacgac atggcgatcc acaccacgcc gccgttcatg ccgacggatg cgaaaatcta
     2401 cgttccgggc caccgcggcc ttgtcggttc cgcgattgtc cgtcgtctgg aaagcctcgg
     2461 ctaccgcaac ctgatcctgc gcaccagggc cgaactggac cttctggatg cccgtgcggt
     2521 ggcaagcctt ttcgcggagg agaagcccga attcgtgttc gtctgcgccg cgaaagtggg
     2581 cggcatcatg gcaaacaaca cgtatcccgc cgagtttttc tacgaaaacg tggcgataga
     2641 aaccgccata attcacgagg catggcgcaa caacgtccgg cgcctgctgt tcctcggctc
     2701 ttcgtgcatc tatccacggg attgcccgca gccgatccgc gaggaatacc tcatgacggg
     2761 tccgctggaa ccgacgaatc gcgcctatgc ggtggccaag atcgccggta ttgaaatgtg
     2821 ccgcgcatac aaccagcagt acggcacgcg gtacctcgcc gcaatgccga caaacctgta
     2881 cgggcagggc gacaattacg atatccagac ctcacatgta ctgccggcgc tcatccgaaa
     2941 aatgcatgag gcgaaaatcc ggggtgatgc cagtgttacg ctctggggga cagggcgccc
     3001 gtaccgggag ttcctgtaca gcgacgatct ggcggatgcg tgcgtgtttc tgctgaacct
     3061 cgatgacgaa cggttcggga tgctgctcgg agatggctct tcaaatccgc tgatcaacgt
     3121 cgggtgcggg gaagacctta cgatcgccga ccttgccggc ctcgtgaaag acgtggtggg
     3181 gttcgagggc accatcgtct gggacacgac aaaaccggat ggtaccccga gaaaactgct
     3241 tgacgtgagc aaactgaacg ggttgggctg gcgaccttcc attcctctgc gggacggaat
     3301 caccagagcg taccggcaat tcctcgaaca cgaaaacaac gcgcgcgggc gttcatgacg
     3361 accgccggcc cgtcagcacg ggtatgaggg gttcaacgac ctgtggactg gtacggtatt
     3421 atcaagacga ccatcggtat cctggcgatt gtgaacccgc tgggagcggt cggaaccttc
     3481 ctcagcgcca cggaaaacct tccggccgcc gcacggcgga aaaccgccga caccgccgca
     3541 gtcaccgtca cggtaacact cctcgtagcg gcgtgggtgg gcgaaacgat ccttcacttc
     3601 ttcggtatca gcatccccgc gttccgcgtc ggcggcggca ttcttgttct cagcatggca
     3661 atctccatgc ttcacgcgcg gccatcaccc gccaaacagg ttcctgaaga agcggcggat
     3721 gcagttgaac ggcaagcagt cggggttgtt ccgctgggca tccctctcat gtcaggtccc
     3781 ggtgcgatcg cccttgtgat tgtggaatct caccgagccc cgacggtgcc ggagcgtctc
     3841 ctcgtgagcc tttgcatcgt gctggttgcc gttgtgtcgt ggacgattct ccgcctcgcc
     3901 ggactgttgg ccaagcgtct cacccgcacc cacatcaaca tctcgactcg tctgatgggc
     3961 cttatcctcg ccgccatcgc cgtggagttc attgccagcg gcgcgctggt cctcttcccg
     4021 gggttgcggt aggcctccta ttccaccgcg aacgtcacac tcaccaccgc cgtcacatcc
     4081 ttttcgcggg acgacgtgtc gttgattccg taatcgctca ccatggtgga gttccgcggg
     4141 gtgatctgga acacacccat gcgtgcgctc cggactgctc ctatcctgga gccaaccgcc
     4201 tgcgcgatgg tttccgcgcg cttgcgggcg tcctgtgcgg cttccgcaag catctccacg
     4261 cgcacgcttg ccagattcga atacacgaac tcgggtggat tcgaggaaat cggcacgcct
     4321 tctttcatga ggtcaggcgc gtcagcggaa agccgggtga tccggtcaac gtcctccgac
     4381 tgaatctcga acccctggaa tatctggtat gcgatgatgc gcccggtcat atgcccgccg
     4441 gaaagcactt cctgaatctg attcgtctgc atcgcctgaa atgtgacagc gctgtcgggg
     4501 attccctgct cacggaagta tgcccgaacg cgttccgagt aacgcttcac gtccccgtac
     4561 gcctcctgca tggagggctt ctgggcggag acgctcccgc gccacaccac ccgatccgat
     4621 gtaattgcct tcttcgcgga gccggttacg gtgatggtgt ctcccgcccg tttcgcgcgg
     4681 ttcacggccg cgtatcccac gaatgacgca atcaccagcg cgagtgccag cgcaagcatc
     4741 ccggcgaatt gctgaggaaa cttctccgac atggcgcctc cttcttttgg gtgagattgc
     4801 ctacgcagaa tataacgacg cattccggac cttcagaacg tggcgcgtcc cagaccccgg
     4861 gaaaagaaga aggggcaagg ttgaacccgc cccttctccg cgtaccggtt gggttcttcc
     4921 ggctcagccc agccgaatgc ggatgcctgc gatttcccat ggccggaacg aacggcgtac
     4981 cgttccgccc tccatgttca accgcacgcc cctgttccat tccatcaggt cggtgtcata
     5041 cgcctcgaca acaggccggt cgaacgtgat cgttacgtcg gttgccgcgc ctgtggtttc
     5101 gtacaggtga agcacccagt cgtcgttgtc ctccgccttc ttcagcgctg cgagaacgat
     5161 gttcggcgct ccaattcgta tcatgctgtg ggtatcgggt cgcgcgcctt cgtgtggaat
     5221 ctcgcgtgtc gtgatgatgg gcgcgttgag catgtgggct tcgcgcaccg tggcagcttc
     5281 agtccagttt cccaggtgag gcagaagtgc gtaggtgaac tcgtgccgcc cttcgtcgga
     5341 ctgatcctgc gatttgtgcg ccacggttgt gcgaagaagg gtgacagtca tcgtgccgtc
     5401 ggcgcggcgg gaatggccgt accgtccgtt gttgagcacc gaaagacccc acttatcgtt
     5461 tgagacgtcc atccacgtct gcgttgcgcg gtcgttgtcc ggaatcggcc gctggacggc
     5521 gccccacggc acttcgcacg tccacggagc atcggccggc agcgcaaccg gaataagcgc
     5581 cttcatcgtg aaattggcct cgtgacaatc gatggtggta tgaacttcca cgcgcggact
     5641 ggtacgggtg atgcggaact cctgcacgaa tgtcgaacga tgccaggtcg ttgtcacgcg
     5701 gattgccgca cgcacgtcgc catcttcgac cacttcggtc cgtacggtct ggttcatcca
     5761 gcgaaccggg ccttccgggc acagatgcca cggatttccg acgtcgccct gtccttcaat
     5821 cgcacaggct tcctttccgt ccgggagcac gttccggttc agccgcttat cgaacaactg
     5881 tgatacccag cccgttcgcg ggttgatcgt tacgcgaacg aagtcgttgt ccagggtcag
     5941 tcggtccgcg cccaccattg atgagaacac tcgttcatca cggtgcgcgg ggtggagcca
     6001 gaccgttttg taccccatgg ccggaatccc atccggctgg acgagaaggc ggaagttctt
     6061 gttcgtcttg tcgaatgtgc cgatatcggt agaaagggtc tgcgccggaa ccggcctccc
     6121 gttcccgtcg cggacttcga acccgtcgca gtagctgtgc ggctcggtga gcaggatctc
     6181 cgccgggtca tttcggtccc acccgagcgt gttgaagagc acgacgggga taccatcccc
     6241 tttggtattg atcgtagagg cgagcacgtt tgccacggcg ttcgttccga tggagcagat
     6301 gctgtccacc tcgtcgtagc gcttgtacgc ctcctcgtag gcttcaaaga tggccgtccc
     6361 cgggaagatg tcgtgcatct ggttgaaaag cgtcaggcgc cacgcccgcg cggcatgttt
     6421 ggtcggataa atcccgccgt cgcgcattgc aagcgtcgcc atcgcctcca gcgtacgcag
     6481 cccccattcc gaacgacggt tgcgcaactt cacctctgcg aaggtgctgt acgcccccac
     6541 gttgttgtgc cggtggtccg ttgtgcttat aaactcgccc tgaaccacgc gcaagccgtc
     6601 cgcggcggta tcaacgga
//
LOCUS       DAOSOO010000177         6608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11714_length_6608_cov_4.671753, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000177 DAOSOO010000000
VERSION     DAOSOO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_6608_cov_4.671753"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4038)
                     /locus_tag="PLT86_11265"
     CDS             complement(<1..4038)
                     /locus_tag="PLT86_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT37231.1"
                     /translation="MKRSCFPGTHVTAALIFLGSVASAVTFTPKLTLPPVQYGAQSWA
                     IPDSSGTYAGKSILALSGQTPGGTLKTYIVWIESQSGVPAVAKIDSSSIPGIVFGQVD
                     WADYNRDGLLDLAISGRTVSATDTLAQDITMVYRQYSDGSFRPEAAFPTGYGMDSTIV
                     RWVDYDNDGDMDLFVSGRKRILSLNPSTGKKEVTGESRLHVFRNDSPTPQVTVFVRDT
                     GVRSRVDQIIPVHDGDADWADIDGNGTPDLAITGKSATFLQGNRVATEIVTEVYLNNP
                     AGVFTRNVLTSLPPRYGGGIAWGDMNEDGLLDIALSGYAKPADGGALTLEVWLHLGEG
                     SFRKQAIQLDQRDAVAGRLVWRDVDNDGRFDLVAMGDSSATVSGIRLYRNNPDLTLSR
                     LSTSLPALGDGAFSLGHLDTDGTVDLIASGRLTPAGASQTTAWSLSGVPANALPTTPS
                     LSPRENRPLVAADRVYFTWGQSSDDANGKTVSYEILLLQGVGTETRVLTAPAAFGPAP
                     HGRKLTYTLLRSLPPGSAMPLYVRARDAAGNTSPWSAPITVYVQDFVNSLETIVPLRS
                     TAASWVDINGDGAPDLVVNGFDQGSNETNRLYVNQGGTLVPNTAYDLPGVYLGSQAWG
                     DVNADGYPDVMLTGSESEQVRITSIFLNRALQSVGLFDENAMTSDKLTGSRAAFGDID
                     NDGDVDFVVTGVSGAALKTLVGENTGVITAQGVVFNVRTLDLTFVDTPAKGLKDGCIS
                     LVDFDQDGDLDLTIQGNTDNGTAAADDDFWAGYLFTFRNTGGMNFSMVNTPAPAGTAT
                     IPAGSALLDQLGDGEHVWADVDNDGDPDLICVGFSRTTAEFVLDNVYWDASALRIYEN
                     TGGGNLVMKQAFPGLWKASLAVGDADNDGKVDILVSGYNDHVVDTGGNHLETPTLRLY
                     VNDGTGRFAEKSVALFSDYGVALGAVRFADVDGDGDLDAIAAGEGVTADGKAVPKSTL
                     FANTNAYFSANRRPNAPTGLAATVGTADSVRLSWNEAIDADPGTNKTPFHTYALRAGT
                     FPGRSNVRSGAEPVGRGTLGYMTHATIRGLADGVYYWSVQAVDNGLARSEWALEQSFV
                     IDTKPPRVARLRGDTLSVGLDRVINVAIDFDDALTGVDTLSAIDVTLSLVGQPSVQVT
                     KLRWDPGDVWFGQASIPTGSFLFEPVRVRVRNVRDIRGNVLADTTFATRLVFTAAQRI
                     AREEGGTITNSAGTISLYVPPRSFDREVGISLAQPDSASLPAGPGPGLGLAVEFTPDV
                     AVGTLAVPAVLTMKYGASPRVARGAVVASNLHIFRLDGSTWRYIGGLADDATKTISVP
                     VNHLGTYALFEASGNVTDATLGDLTC"
     gene            4277..5482
                     /locus_tag="PLT86_11270"
     CDS             4277..5482
                     /locus_tag="PLT86_11270"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT37232.1"
                     /translation="MISADIAPKLATETGSKIVLMIMDGLGGFPNETGKTELETAHTP
                     NLDTLAAEGATGLIDTLGRGIPPGSGLAHLALFGYDAFVHDIGRGAIAANGIGLTLKP
                     NQVAARLNFCTQQNGLITDRRAGRIPTEKCAELCRVLNAKVSVPGVRIEAHPVMDYRA
                     VVVFEGDGLSIEIADTHPGATGVPAKPVKATGAGAEKMVGVVEAFIAQAKEALAALHP
                     ANMILLRGFAVQPDFPSMQACYKLKPACIATYPDYRGVAKMIGMDILKTGMEIAEEVD
                     TLEQDWKSDHTFYFFHVKKTDSYGEDGNFDGRVHKIEEVDALIPRIMALKPDVFAVTG
                     DHSTPAVFKAHTWHPVPVLLWGKTVRRDSTTSFGERAAICGGMGRFEGSALMLELLAH
                     AGKLKKMGA"
     gene            5870..>6608
                     /locus_tag="PLT86_11275"
     CDS             5870..>6608
                     /locus_tag="PLT86_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37233.1"
                     /translation="MIAKKAARRALSVFCSLFCATNALAADIVPRPIVGEIAGAVRIP
                     ARVAIVMSAEERARVYAWAGYRLNIGTPVENTLLQALAQVFDSVCVVPTLPAPSDVGE
                     EAALFVEPRVDRFQLSQSLFSLSQVRCEMAVQYVAVGRRRGVLVEIAQYRINVPRITV
                     WSEHFGGSFETAIKEAASRTTAAAARGLVEAMSVDDTLARFVREHFPAPSPTGASPVF
                     SMTSFDPNRLSIAVLDLRPLVVTADEAT"
BASE COUNT         1274 a         2089 c         1994 g         1251 t
ORIGIN      
        1 gcaggtaagg tcgccgagtg tcgcgtccgt tacgttcccg gatgcctcaa acagggcgta
       61 cgttccgagg tggttgaccg gcaccgagat agttttcgtc gcgtcatccg caagcccgcc
      121 gatatacctc catgtggaac catccaggcg gaagatgtgc agattcgacg ccaccaccgc
      181 ccctcgcgct acccggggcg aagcaccgta tttcatcgtc agtaccgccg gaaccgcaag
      241 cgtgcccacc gcaacgtcag gcgtaaactc caccgccaga ccgagtcccg gccccggccc
      301 ggcggggagg ctcgcggaat ccggttgcgc aagcgaaatg cctacctcgc ggtcgaaact
      361 ccgcggtgga acataaaggc ttatggttcc tgccgagttc gtgatcgtac cgccctcctc
      421 ccgcgcgatg cgctgtgctg ccgtgaagac gagccgggta gcgaaggtgg tatcggcgag
      481 gacgttaccg cgaatatcac gtacgttccg gacgcgcacc ctgaccggct caaacaggaa
      541 actgccggtc ggaattgatg cttgcccgaa ccagacgtcg cccgggtccc accggagctt
      601 ggtgacctga acggaaggct gtccgaccag cgagagcgtt acgtcaatcg cggaaagggt
      661 gtcgacgccg gtcagcgcgt catcgaaatc aatcgcgacg ttgatcacgc ggtcgaggcc
      721 gacgctgagc gtgtcaccgc gcaggcgggc aactctcgga ggcttcgtgt ctattacgaa
      781 ggactgctca agcgcccatt ccgagcgcgc aagcccgttg tccaccgcct gcacgctcca
      841 gtagtacacc ccgtccgcca gtccgcggat cgtcgcgtgg gtcatgtacc cgagcgtgcc
      901 ccgtcccacc ggctccgctc cggaacggac gttgctccgt ccgggaaacg tgcctgcgcg
      961 cagtgcgtac gtgtggaacg gcgttttgtt cgtgccgggg tccgcgtcaa tggcttcgtt
     1021 ccacgagagc cgcacggaat ccgccgtacc gacggtggca gcgagaccgg tcggtgcatt
     1081 cgggcgacga ttggcgctga agtacgcgtt tgtgttcgcg aacagggtgc ttttcggcac
     1141 cgctttgccg tcggccgtta cgccttcacc cgcggcgatg gcgtcgagat caccatcacc
     1201 atccacgtcc gcgaatcgca ccgcgccgag cgcgactcca tagtcgctga acagagcgac
     1261 agacttttcc gcgaaccggc cggttccgtc gtttacgtaa agccgcagcg ttggcgtttc
     1321 gaggtgattc ccgcccgtat caacgacatg atcgttgtac cccgaaacca gaatatccac
     1381 cttgccgtcg ttgtccgcat ccccgacggc aaggcttgcc ttccagaggc ccggaaacgc
     1441 ctgcttcatc acgagattgc cgccgccggt gttctcgtag atgcgaagcg cgctggcgtc
     1501 ccagtacacg ttatccagca caaactcggc ggttgttcgc gaaaagccca cgcagatgag
     1561 gtccggatcg ccatcgttgt ccacgtcagc ccagacatgt tcgccatcac cgagttgatc
     1621 aagcaacgcg cttcccgcgg gaatggttgc cgttccggcg ggggccggcg tgttcaccat
     1681 gctgaaattc atcccgcctg tgttccggaa ggtgaacagg tatccggccc agaaatcgtc
     1741 gtcggccgcg gcagtgccgt tatccgtatt gccctgaatc gtgaggtcca gatcgccgtc
     1801 ctgatcgaag tcaaccagcg agatgcaacc gtctttgagc cccttcgcgg gagtgtcaac
     1861 gaaagtcaga tccagcgtgc gaacattgaa aaccaccccc tgcgcggtga taacgccggt
     1921 gttctcgccc accagtgttt tcaatgctgc tcccgacaca cccgtaacga cgaagtccac
     1981 atcgccatcg ttgtcaatat cgccgaatgc ggcgcggctc ccggtaagtt tgtccgatgt
     2041 catggcgttt tcgtcgaaca acccgaccga ttgcagcgca cggttcagga agatgctcgt
     2101 gatgcgcacc tgctcagatt ccgatcccgt caacatcaca tcggggtagc cgtccgcatt
     2161 cacgtcgccc caggcctgac tgccgaggta cacaccggga aggtcatacg ccgtgttggg
     2221 aaccagagta ccgccctggt tcacataaag ccggttcgtt tcgttgcttc cctggtcgaa
     2281 gccgttcacc accaggtcgg gcgcgccatc gccgttgatg tccacccatg aggccgccgt
     2341 actgcgcaac ggaacgatcg tttccagcga gttcacgaaa tcctgcacgt acaccgtgat
     2401 gggcgcgctc cacggagacg tattgcctgc ggcatcccgc gctcgaacgt acagcggcat
     2461 cgccgagccg ggcggcaacg agcgcaggag ggtgtacgtg agcttcctgc cgtgcggtgc
     2521 cggaccaaag gccgccggcg cggtcaggac gcgggtttcg gtccccacgc cctgcagaag
     2581 cagaatctcg tacgacaccg tcttcccgtt tgcatcgtcc gacgactggc cccaggtgaa
     2641 gtaaacccgg tcagccgcca ccagcgggcg attttcccgc ggcgaaaggg agggcgtcgt
     2701 cgggagagcg ttcgccggca cgccggaaag gctccaggca gtggtctggc tcgcccctgc
     2761 cggggtgaga cgcccgctcg cgatgagatc aaccgtgccg tcagtgtcca gatgtccgag
     2821 agagaacgcg ccgtccccaa gcgccggcaa cgaggtggac agacgggaaa gcgtgagatc
     2881 agggttgttc cggtacagcc gaatgccgct cacggtggcg ctggagtcgc ccatcgcgac
     2941 gagatcaaag cggccgtcat tgtccacgtc cctccacacg agtcggcccg ccaccgcatc
     3001 gcgctgatcg agctgaattg cctgtttgcg gaaggaccct tcgccgaggt ggagccacac
     3061 ttccagcgtc agcgcgccgc catcggccgg tttcgcgtac ccggagaggg cgatatccag
     3121 cagcccgtcc tcattcatgt cgccccacgc gatgccgcct ccgtaccgcg gggggaggga
     3181 agtcagcaca ttccgcgtga acacgcctgc cgggttgttg aggtagacct cggtcacgat
     3241 ttccgttgcc acgcggttcc cctgaaggaa cgtggcgctt tttcccgtga ttgcaagatc
     3301 gggcgtgccg tttccgtcta tgtcggccca gtcggcatca ccgtcgtgca ccggaatgat
     3361 ctggtccacc cggctgcgca cgccggtatc ccgcacgaac acggtcacct gcggcgtagg
     3421 agagtcatta cgaaatacat gtagacggct ttcccccgtc acttctttct tacctgtgga
     3481 cgggttcagc gaaaggattc gttttcggcc gctgacgaaa agatccatgt ctccgtcgtt
     3541 gtcataatcc acccaccgca caatcgtgga gtccatcccg tacccggtgg ggaaggccgc
     3601 ttccgggcgg aacgatccgt cggagtactg gcggtacacc atcgtgatgt cctgggcgag
     3661 ggtatccgtc gcgctcaccg ttcgcccgct gatggcgagg tccagcagac catcccgatt
     3721 gtaatccgcc cagtccacct gcccgaacac gataccaggt atgctggatg agtcgatttt
     3781 cgccaccgcc ggaactcctg actgcgactc aatccagacg atgtacgtct tcagtgtgcc
     3841 gccgggcgtc tggccagaga gggcgagaat gctcttgccg gcgtaggtgc cggagctgtc
     3901 gggaatcgcc cacgactgcg ccccgtactg cacggggggc agggtgagtt tcggcgtaaa
     3961 tgtcaccgcg ctcgcaacag aaccgaggaa aatcaatgca gcagtgacat gggtgcccgg
     4021 aaagcaggac ctcttcatgg cgtaccatca gggaagcgag gttgtacgct acgccgtaac
     4081 cgctctgaag ttcagcggtt cacggtctca gtcacgccat aaataacgca ttttgctttc
     4141 tacgtcaagc gagccctgcg ccgcgccgca ccttgtccgc gttcacacgg actgcgtagt
     4201 tttctcagag ccgcgtggcc gagggaattg gccgcgcatt tctggtggga ttttcacacc
     4261 tgatgcgagg aatgccatga ttagcgcaga catagccccg aaactcgcca ccgaaacggg
     4321 ttcaaagatc gtccttatga tcatggacgg acttggcggg ttcccgaacg aaaccgggaa
     4381 aaccgaactg gaaacggcgc atactccgaa cctcgatacc cttgccgccg agggcgcgac
     4441 cggccttatt gatacgctgg ggcgcggcat tcctcccggc agcgggcttg ctcacctcgc
     4501 gctgttcggg tacgacgcct tcgtgcacga catcggccgc ggtgcaatcg ccgccaacgg
     4561 gatcgggctc acactcaagc cgaatcaggt cgccgcgcgc ctcaacttct gcacgcaaca
     4621 gaacggcctc atcaccgacc ggcgggcggg ccggattccg acggaaaaat gcgcggagct
     4681 gtgcagggtc ctcaacgcga aggtgagtgt gccgggcgtc aggatcgaag cacatcccgt
     4741 catggactac cgggcggtgg tggtgtttga aggtgacggc ctctccatcg agatcgcgga
     4801 cacccatccc ggagccacag gcgttccggc gaaacctgtc aaagccaccg gcgccggcgc
     4861 ggaaaagatg gtgggcgtcg ttgaggcatt tatcgcgcag gcgaaggaag cgctggcggc
     4921 gctgcacccc gcgaacatga ttctgcttcg cgggtttgcc gtgcagcccg atttcccctc
     4981 catgcaggcc tgttacaaac tcaaacccgc ctgcatcgcc acctacccgg attaccgtgg
     5041 cgtcgcgaag atgatcggca tggacatcct gaaaacaggc atggaaatcg ccgaagaggt
     5101 ggacacactc gagcaggact ggaaaagcga ccacaccttc tacttcttcc acgtgaaaaa
     5161 gaccgacagc tacggcgagg acggcaactt cgacggccgc gtgcacaaaa tcgaggaagt
     5221 ggacgcgctc attccgcgca ttatggcgct gaagccggat gtgttcgcgg ttacgggcga
     5281 tcactccacc cccgcggtgt tcaaggcgca cacctggcat ccggtgccgg tgctcctctg
     5341 gggcaagacc gtccggcgcg actccaccac ctcattcggc gaacgggctg caatctgcgg
     5401 cgggatgggg cggtttgaag gttccgcact gatgctggaa ctcctcgcgc acgcaggcaa
     5461 actgaagaaa atgggcgcgt aaccccgcca aaagagccaa tccggccagg atggagggac
     5521 acgctctgtc gtgtccccct ttttttccgc agggggcgcg ctggattccc gccttcgctt
     5581 cgtgctctgc cgagcacgcg ggaatgacat ctttcagatg ccgacggcag ggaaaaagcc
     5641 gggtacctgt tgcatcaaga aatggcgacg cggacggtac cgtggtctcg acgtcgttcc
     5701 cgggcacgcg catcctacgc tcagtcgtca ttcccgcgaa ggcgggaatc cagtagtcag
     5761 aagagcacca ttcccaaagg ggtgtacaag acccggctcg gttctatctt attcagtgtc
     5821 gggtggcgca acgacaaagc ctacttgcat ccatcgtggg gaagtctgga tgattgcgaa
     5881 gaaagccgct cgtcgtgctc tttccgtgtt ctgcagtctc ttttgtgcca caaatgcctt
     5941 ggccgccgac attgtcccgc ggccgatcgt aggggagatc gcgggtgccg ttcgcatccc
     6001 cgcgcgcgtg gcaatcgtca tgagcgccga ggaaagggcg cgtgtctatg catgggcagg
     6061 ttacaggctg aacatcggta ctcccgtcga aaacacgctg ctccaagcgt tggcgcaggt
     6121 cttcgattca gtgtgtgtgg tgcctacatt gcccgcgcca agcgacgttg gagaagaagc
     6181 cgcgctgttc gtagaaccaa gggtagatcg tttccaacta tcacagagtt tgttctcgct
     6241 cagccaggtc cggtgcgaga tggcggtgca atacgtcgcc gttggtagac ggcgaggtgt
     6301 tctcgtcgaa attgcgcaat accgaatcaa tgtgccaaga ataactgtgt ggagcgagca
     6361 cttcggggga agctttgaaa cggcaattaa ggaagctgcc tcccgcacaa ccgcagcggc
     6421 cgctcggggt ttggttgagg cgatgtccgt agacgacacc ctcgcgcgtt tcgtgcgtga
     6481 gcatttccct gctccatccc ccactggtgc ttctccggtc ttctccatga cgtcgttcga
     6541 cccaaaccgc ctcagcatcg cggttctcga ccttcgcccg ctggttgtca ctgcggatga
     6601 ggcgacga
//
LOCUS       DAOSOO010000178         6597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11744_length_6597_cov_5.794558, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000178 DAOSOO010000000
VERSION     DAOSOO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_6597_cov_5.794558"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLT86_11280"
     CDS             complement(<1..437)
                     /locus_tag="PLT86_11280"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOT37234.1"
                     /translation="MTPIPVVQLAALLGAEWNGDPHLVITGVAPLSEAGTTELSFLAD
                     PRFAAEARSSAAGAILARPGTPDVPRARCLAVPDPYSAMYRSVMLLHPSTTRGSFLSP
                     QAIVASTANVDKSAYVGAFVTIGNGTTVGERAVIREGVRIGEKV"
     gene            complement(462..986)
                     /locus_tag="PLT86_11285"
     CDS             complement(462..986)
                     /locus_tag="PLT86_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT37235.1"
                     /translation="MRKMRWVVPVVAAVCSLFLGASRAQAQSFKVAVIYMDEIREKGQ
                     PYIEALQRLQQVSQQRQQEAQTKQGEIQRLQEQLQRQASLMTEQRRTQSEAQIRQKVT
                     EYEQWAGQAQQELQQQQIQAIQPIDERVLQIVERIANERGIDVVLDGVAVAFIKNKEQ
                     NNLTNAVIQALNQQ"
     gene            complement(1028..3313)
                     /gene="bamA"
                     /locus_tag="PLT86_11290"
     CDS             complement(1028..3313)
                     /gene="bamA"
                     /locus_tag="PLT86_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOT37236.1"
                     /translation="MHYHRILVPGVLLCLCTWSLSRAARVDSLIVRGNVRASETLIRS
                     ASGLSTGAEILIPQIQAAIRNLYATGVFSDVVIKREAIGFNREKLIIEVTEYPVLNNL
                     TFSGNRKLKRDDISKKVRILPGQVVNPQALTRAIADIQKEYRDKGYYLARVVPEQKPA
                     GPGRVDLNLAIREGDRVQVERIRFIGARAFNPRKLRDQMSTRQNRWWRSADFDEEKLR
                     EDKEKILAFYRKFGYREAAIVRDSVYLDDSKKKLFVDLFVDEGPQYHFGRITWAGNLL
                     LSDEQVHRVLRLREGDVYNREAFDEAVFRLSSAYQEQGYWAMDPGIMETPRGDTVDVS
                     FNIVESEPSRVQFVDILGNDKTKDKVIRREMTLVPGDIFRRSDLERSNRNVFYLNYFE
                     NVEPNIRPAAGGNVDVTMTVKEKPTGTVNMAVGYGEADKWVGSIGLSIPNLRGNGQQA
                     DFQWEFGQVTTSFYLSFTEPWLFDTPTSGSITLFNTRREYDVTEESRGFSLRAGRRLR
                     WPDDYSRVYASYSLRTEDYTFPLDYTDADKLAYISNPSDPHLISSALGIGYVRDSRNL
                     PLFPTDGTYFSYDLQMAGGPVGGDVSYRKHTIELDYFLPLFEIKGWGPALALKTTFGQ
                     INTAQPLAVPLSERFRPGGVSFDGQIRGYYDYAVGPKSEYGSSTGGYTMLLTTAELSF
                     PVVQQQIYGVLFADAGNAWRGLAEMNPYRLKRSYGFGARFILPLAGVIGLDFAKGVDP
                     DPYSGGGGWETHFQFGPTIMR"
     gene            3651..5384
                     /locus_tag="PLT86_11295"
     CDS             3651..5384
                     /locus_tag="PLT86_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOT37237.1"
                     /translation="MPETSRSFVLVLHTHLPYVLSHGKWPHGMDWLNEAAAECYLPLL
                     NAFERMVSRGVSPRVTIGITPVLAEQLADAAFKDDFGGYLRMKVDAAKTDEAYFHKIG
                     DTQREELACYWQMVYRQLIDDFENYSRDIIGSFRELQDGGHIEIMTSAATHGYLALLG
                     KDECVRAQVRQGVETYRRHFGRSPRGIWLPECAYRPPYNWTTPVPSEIEPADRLGIDS
                     ILSDNGLEYFVLDTHLLRGGKAIGAYIDRFGALKALWEQFEEAYPDAKKERDLSPLLP
                     YYAGGVLPGQKPVAFFTRHPECSLQVWSGEWGYPGDGAYLDFHKKHFPGGHRYWAVTG
                     RRVDLGDKVLYDPAAVEARLEENADHFVDLVGKALDGQGAGNPALVCAPFDTELFGHW
                     WFEGPRWIEKVLTKMAERADYQPATGSDALKAIPPREILSVPEGSWGEGGYHYIWLND
                     LNKWTWTHVYDAERTYCALVEELATSTLPEVHRVLKQAARELLLLESSDWQFLISTWS
                     ARDYAELRFTHHWETIKRLFGIMERLARRERLEESDWLFVEECEAKDGLFPDIEPRWW
                     MPEKRAVPGIS"
     gene            5398..>6597
                     /locus_tag="PLT86_11300"
     CDS             5398..>6597
                     /locus_tag="PLT86_11300"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOT37238.1"
                     /translation="MHREIRVFMVAAEGVPFAKTGGLADVVGALPYALAEHGCSVRCF
                     LPRYRQVDLEKFGLSTLLPRRTVSFAGAEVAYRVLAAPAERGVEWLFLDAPGFYDRDA
                     LYGTYGEDYPDSCERFAFFCQQALATVRELDWRPDVVHCHDWQSGLIPAYLKLKTRTG
                     IIDTFDTFFSEIRSLFTIHNIGYQGMFSADKLWITGFSPEHFTYDKLEYWGSINFLKA
                     GIVFADRISTVSKRYSEEIQTEAFGRGMDGVLRARAADLTGILNGIDVETWNPATDPH
                     IAANYTAEDLRGKRECRRALSKEVGLNPRVRVPLIGIISRLEEEKGFDLLSSAIPRIM
                     AMGAQMVVLGTGSPEYHTILSRMARRYSGQFAAVLAFDPVLAHRVEAGADMFLMPSKY
                     EPCGLTQM"
BASE COUNT         1335 a         1790 c         2002 g         1470 t
ORIGIN      
        1 accttttccc cgattcgtac gccttcacgg atcacggcgc gttcgccaac ggtggttcca
       61 ttaccgatgg tgacaaacgc gccgacatac gcgcttttgt cgacgttggc ggtggaagcc
      121 acgattgcct gcggcgaaag gaaagatccc cgcgttgtcg aaggatggag caacatgacg
      181 gagcgataca tcgcggagta gggatcaggc acggcgaggc acctggcgcg ggggacgtcc
      241 ggggtgccag gacgggcaag aattgcgccg gctgcgctcg aacgggcttc cgccgcgaat
      301 ctggggtcgg cgagaaacga gagctccgta gtcccggctt cagagagggg tgccacccct
      361 gtgatgacga ggtgcggatc gccgttccat tccgctccca gaagcgcggc cagttggacg
      421 accgggatcg gcgtcatctg gttccctcac cggaacgcgg actattgctg attcagcgcc
      481 tgaatcacgg cgttcgtgag attgttttgt tctttgtttt tgatgaaagc caccgcaacg
      541 ccgtccagta ccacatcgat accccgttcg ttggcgatcc tctcgacaat ttgaaggacg
      601 cgctcgtcga tcggttggat tgcctgaatc tgttgttgct ggagctcctg ttgcgcctga
      661 ccggcccatt gttcgtattc ggtgaccttc tgccggattt gcgcttccga ttgcgtacgc
      721 cgttgctccg tcatcagact cgcctgacgt tgcagttgct cctgcaatcg ctggatctcc
      781 ccctgcttcg tctgggcttc ctgctggcgc tgttggctca cctgctgaag ccgctgcagg
      841 gcttcgatgt agggttgtcc cttctcgcga atttcatcca tgtagatgac cgcgactttg
      901 aaactctgcg cctgtgcgcg agatgcgccc aggaaaaggc tgcagacggc tgctacaaca
      961 ggaaccaccc atctcatttt gcgcatagga aacatctcct tctggaaatc gtggttacgg
     1021 aaatcgctca cctcatgata gtcgggccaa actggaagtg tgtttcccac ccgccaccac
     1081 cggagtaagg atccgggtca accccttttg cgaagtcgag gccgatgacg ccagccaggg
     1141 gaagaatgaa tcgggcaccg aatccgtatg agcgcttgag tcggtacggg ttcatctccg
     1201 ccaggccgcg ccaggcattt ccagcgtccg caaaaaggac gccgtagatc tgttgttgaa
     1261 caacggggaa gctcagctcc gctgttgtga gcaacatcgt gtagccgccg gtgctgctgc
     1321 cgtattcgct cttcggcccg acggcatagt cgtaatatcc gcgaatctgt ccgtcaaaac
     1381 tcaccccgcc cggacggaat cgctcgctga gaggtacggc caagggttga gcggtgttga
     1441 tttggccgaa tgttgttttg agggccaacg cggggcccca acccttgatt tcgaacaacg
     1501 gtagaaaata gtcaagttcg attgtgtgct tccggtaaga gacatcccca ccaaccggtc
     1561 cgcccgccat ctgcaggtcg taggagaaat atgttccatc agtggggaag aggggcagat
     1621 tccgcgaatc tcgcacgtaa ccgattccca aggcggaact tataaggtgc ggatcggatg
     1681 gattgctgat gtacgcgagt ttgtccgcgt cagtgtagtc gagcgggaac gtgtagtcct
     1741 cggtgcggag tgaatagctt gcgtacacgc gggagtagtc gtcgggccaa cgaaggcgcc
     1801 tgccggcacg cagactgaac ccccggctct cctcggtaac gtcatactcc ctgcgggtgt
     1861 tgaacagcgt tatggagccc gatgtcggag tatcgaacag ccatggttca gtgaaactca
     1921 ggtaaaaact ggtggtaacc tggccgaact cccactggaa atcggcctgt tgaccgtttc
     1981 ctctcagatt ggggatggat aggccgattg aacccaccca tttgtcggcc tcgccgtaac
     2041 cgactgccat gttgaccgtt cctgtcggtt tttccttcac cgtcatggtg acgtcgacgt
     2101 ttccgccagc ggccggtctg atattaggct cgacgttctc gaagtagttg aggtagaaga
     2161 cgttccgatt gctccgttcg agatccgaac gacggaaaat gtcgcccggc acgagggtca
     2221 tttcgcgccg gatcactttg tcctttgtct tgtcgttccc cagaatgtcc acgaactgaa
     2281 cccgggaggg ttcggactcc acaatgttga acgagacgtc gaccgtatct ccgcgcgggg
     2341 tttccatgat tccgggatcc attgcccagt agccttgttc ctggtacgcg gatgacagac
     2401 gaaacactgc ttcgtcgaac gcttcccggt tgtagacgtc cccctcacgg aggcgaagaa
     2461 cacgatgcac ctgctcgtcg gacaacaaca ggttgcccgc ccaggtgatg cggccgaaat
     2521 gatactgcgg gccttcgtca acgaacagat cgacgaacag ctttttcttg gagtcatcga
     2581 ggtagacaga atcacgaaca atcgccgctt cgcggtaccc gaacttgcga tagaaggcca
     2641 gaattttctc tttgtcttcc cgaagttttt cctcgtcgaa gtcagccgac ctccaccagc
     2701 ggttctgtcg cgtgctcatc tgatcgcgca gtttccgcgg attgaaggcg cgtgctccga
     2761 tgaaacgaat ccgctccacc tgcacgcggt caccttctcg gatcgcgaga ttcaggtcca
     2821 cccttccggg cccggcaggt ttctgttccg ggacgacccg cgcgaggtag taccccttgt
     2881 ctcggtactc cttctggata tcggcgatgg cgcgggtgag ggcctgtgga tttaccacct
     2941 gacctggaag aatgcgcact ttcttggata tatcatctcg tttgagtttt cggtttcccg
     3001 aaaacgtaag gttgttgaga acagggtatt cggtaacctc aatgatcagc ttctcgcgat
     3061 tgaatccgat cgcttctcgt ttgatcacga catcgctgaa cacgcccgtt gcgtacaggt
     3121 tcctgatggc cgcctggatc tgcgggatga ggatttccgc gccggtggaa agcccggatg
     3181 cggaacgaat gagtgtttcg gatgcccgga cattgccacg aacaatcagg ctatcaacgc
     3241 gtgccgcacg ggaaaggctc cacgtgcaca gacacagcaa aacccccggt accaagattc
     3301 ggtgataatg cacgattcgc ggacctcacg ggagggattc gggtgttcga tcgcatcgat
     3361 ttacaggctt ctgtctcgcc cgggaaagac taaaaataca gtgtgacgag tttcctgcac
     3421 agtggttggt gccgaagaag cggttggaga gtcgagacgg catgcacatg tgacgcggag
     3481 ggttcgaact cagttccatc gacacagcgc gtgcgacgct gcgcgttgga gtcatttgcg
     3541 tattatatgg tacgggcgtg caagattgtg aacacgtctg tgacgctcgc caggaaaagt
     3601 gcttcgtggt ttttcatttc tgcgcattcg aaccggggag taatagtttc atgcctgaga
     3661 ccagccgttc cttcgttctc gtgcttcata cgcacctgcc ttacgtcctt tctcacggca
     3721 agtggcccca cgggatggat tggttgaatg aggccgccgc cgagtgctac ctgcctttgt
     3781 tgaacgcgtt tgaacgaatg gtgtcgcgag gtgtttcgcc gcgtgtgaca atcgggatta
     3841 cgccggttct tgcggaacag ctggccgacg cggcgttcaa ggatgatttc ggcgggtacc
     3901 tccggatgaa agtggatgct gcgaaaactg acgaggcata tttccacaaa attggcgaca
     3961 cgcagcgtga ggaactggcc tgctactggc agatggtgta ccggcagctc atcgatgatt
     4021 tcgagaacta ttcacgagac atcatcgggt cgttccggga gttgcaggac ggcgggcaca
     4081 tagaaatcat gacgagtgcc gccacgcacg gatatctcgc acttcttggg aaagacgaat
     4141 gtgttcgggc gcaggtccgg cagggagtgg aaacgtaccg ccgtcacttc gggcgatcac
     4201 ccagaggaat ctggctcccg gaatgcgcgt atcgcccgcc gtacaactgg accacgcctg
     4261 tgccgtcgga gatagaaccc gcggatagac tcgggataga ttcaattctg agcgacaacg
     4321 gcctcgaata cttcgtgctg gatacacatc ttctgcgggg cggcaaagct atcggagcct
     4381 acattgaccg cttcggcgcg ttgaaagccc tctgggaaca gttcgaagaa gcgtatccgg
     4441 acgcaaaaaa ggagcgcgac ctcagcccgt tgctgccgta ctacgcggga ggggtcctgc
     4501 ccgggcaaaa acctgtggcc ttcttcacgc gtcatccgga gtgcagcctt caggtgtggt
     4561 ccggagaatg gggatacccg ggagacgggg catacctgga tttccacaag aaacactttc
     4621 caggcgggca ccgctactgg gcagtgacgg gccggcgtgt tgaccttggc gacaaggttc
     4681 tttacgaccc ggctgcggta gaggccagac tggaagagaa tgccgatcat tttgtggacc
     4741 ttgttggaaa agcgcttgac ggccagggtg cggggaatcc cgcgcttgtc tgcgctcctt
     4801 tcgacaccga attgttcgga cactggtggt tcgaagggcc gcgctggatc gagaaagtgc
     4861 tgaccaagat ggcggagcgt gccgattatc aacccgcgac gggcagcgat gccctgaagg
     4921 cgattcctcc gcgggaaatt ctgagcgtgc cggagggttc ctggggtgag ggtgggtacc
     4981 actacatctg gctgaacgat ttgaacaagt ggacgtggac ccacgtatac gatgcggaac
     5041 gaacatattg cgcacttgtt gaggagctgg caacgagtac tctgccggag gtacaccgtg
     5101 tgctgaaaca ggcggcgcgc gagcttctcc tgttggagtc atcggactgg caatttctga
     5161 tcagtacatg gtccgcccgg gattacgcgg aactgaggtt cacccaccac tgggagacaa
     5221 tcaaacgcct tttcgggata atggaacgtc tggcgcggcg tgagcgtctg gaggagagtg
     5281 actggttgtt tgttgaggaa tgtgaggcca aagacggtct tttccccgac atagagccca
     5341 gatggtggat gccggaaaaa cgggcggtgc ccggcatcag ttgaggggag gatgcgtatg
     5401 catcgcgaaa taagggtgtt catggtcgca gcggagggag ttccgttcgc gaagacgggg
     5461 gggcttgcag acgttgttgg tgccctgccc tacgcccttg ctgagcacgg gtgcagcgtt
     5521 cgttgcttcc tgccgcgcta ccgccaggta gatctcgaga agttcggtct tagtacgctt
     5581 ctgccacggc gcacagtttc ctttgcgggg gcggaagttg cgtaccgcgt tctcgcggct
     5641 cctgcagaga ggggcgtgga gtggttgttt ctggacgcgc cgggttttta cgaccgggac
     5701 gcgttatacg gtacctacgg tgaagactac ccggattcgt gcgaacgctt cgcgtttttc
     5761 tgtcagcagg cgctggcgac tgttcgcgaa ctggactggc gtcctgacgt tgtgcactgc
     5821 cacgattggc aatcagggct gattcctgcg tatctgaaac tgaagacgcg taccggtatt
     5881 atcgacactt tcgacacgtt cttttcggaa atcagaagcc ttttcacgat tcacaatatc
     5941 ggctatcaag ggatgttcag cgcggacaag ctgtggatta ctgggttttc gccggagcat
     6001 ttcacgtatg acaaactcga atactggggc tccatcaact tcctcaaggc cgggatcgtt
     6061 tttgcggacc gcatctccac ggtgagcaag cgctacagcg aggaaatcca gactgaagcc
     6121 ttcggcagag gtatggatgg cgtgttgcgc gcgcgagcag ccgatttgac gggaatcctg
     6181 aacggcatcg acgtagagac gtggaatccg gcgactgatc cacacatcgc ggcgaattac
     6241 accgcggagg atctgcgcgg aaagcgggaa tgtcggcgcg cgttgtcgaa agaagtggga
     6301 ctgaaccccc gcgttcgtgt tccgctcatt ggcatcatca gccggctcga ggaggaaaag
     6361 ggcttcgatc ttctttcctc tgccatcccc cgcataatgg cgatgggcgc gcaaatggtt
     6421 gttctgggaa cgggttctcc ggagtatcac acgatacttt ctcgaatggc gcgacgctat
     6481 tcggggcagt ttgccgcggt gcttgccttc gacccagtgc tcgcacatcg ggtcgaggcg
     6541 ggtgccgata tgtttttgat gccctcgaaa tatgaaccgt gcgggctcac ccagatg
//
LOCUS       DAOSOO010000179         6582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_11783_length_6582_cov_5.305960, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000179 DAOSOO010000000
VERSION     DAOSOO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11783_length_6582_cov_5.305960"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLT86_11305"
     CDS             <1..377
                     /locus_tag="PLT86_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT37239.1"
                     /translation="RILTVASVAGFQAMPYMAEYAATKAYVLSLSEALRVELAPYGVV
                     VACLAPGPTDTDFFSSAGTTMLNTPGRKTPEEVARVGFTAMLRGKPLAVPGAMNKLMV
                     FAGRLVPRTLFACAAARVLRRR"
     gene            complement(462..1277)
                     /locus_tag="PLT86_11310"
     CDS             complement(462..1277)
                     /locus_tag="PLT86_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37240.1"
                     /translation="MPFPMTSIEHVQVSRVLCGTNPFFGFSHFSAARDAWLRNHFTKE
                     RILEVLEACRLEGINGTISSPNPFYHEILREHERQTGHHWVWMCTPGGNDWKEVEPGV
                     KWCADHGVEICLPHQGFTDNNLIPAENRIAGGPEILQCIRDNGMIPGWSTHRPETIIV
                     SDKAGYDVATYILPYNSIGFLCPVETDWTGRVIRETPKPVICIKPLGAGRVMPPTGLS
                     FVFGSNKPTDTVAIGFLGPGEAKEDITIARDLIEGREREYELQYTRSKASLVR"
     gene            complement(1424..1987)
                     /gene="frr"
                     /locus_tag="PLT86_11315"
     CDS             complement(1424..1987)
                     /gene="frr"
                     /locus_tag="PLT86_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT37241.1"
                     /translation="MATESQIVRQTRAKMQKTLETIVHEFSTVRTGKANPALLDVVRV
                     EAYGSKVPISQVANVTAPEPAMLLVQPWDKSTIGAIDKAIRLSDLGLNPTNDGNVIRI
                     ALPPLTEERRKEYVKIVHKMAEDGRIVIRNERRDAIEELRKMEKAKQVSEDDSRRDQK
                     EIQTLTDEFIKKVDAALAAKEKEIMEV"
     gene            complement(2013..2726)
                     /gene="pyrH"
                     /locus_tag="PLT86_11320"
     CDS             complement(2013..2726)
                     /gene="pyrH"
                     /locus_tag="PLT86_11320"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT37242.1"
                     /translation="MSAFKRILLKLSGEALQGSHAFGIDPVEVSRICQEIVEVHSRGV
                     QIGLVVGGGNIFRGKEIVNQLGVEAASADTMGMLATVINCLALQAALEKLGLETRVQT
                     AIRMDQVAEPLIRRRAIRHLEKERVVLFAGGTGSPFFTTDTAAALRAVEIGAEVLLKG
                     TKVDGVYDDDPARNPGANLFDEITYEDILQHGYKVMDATAVSLSMDNRLPIVVFNLTR
                     AGELVRAALGDRVGTRVVA"
     gene            complement(2782..3375)
                     /gene="tsf"
                     /locus_tag="PLT86_11325"
     CDS             complement(2782..3375)
                     /gene="tsf"
                     /locus_tag="PLT86_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT37243.1"
                     /translation="MEITAAQVKELREKTGAGMMECKKALGETNGDLQKAIDYLREQG
                     ILKSAKLGSRATSQGAIFSYVHPGDQLGVLVEINCETDFVARTDDFKALGKNIAMQVA
                     ASAPQYVSRDQVPQEVLDHEKEVSRAQAKAEGKPEKILDKIVEGRLEKYFQEVCLLDQ
                     AYIRDDSRKVSDLVTEAIAKIRENITIKRFVRFKVGE"
     gene            complement(3459..4268)
                     /gene="rpsB"
                     /locus_tag="PLT86_11330"
     CDS             complement(3459..4268)
                     /gene="rpsB"
                     /locus_tag="PLT86_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT37244.1"
                     /translation="MARVEMKDLLEAGVHFGHQTRRWNPKMKRFIFMERNGIHILDLN
                     KTIALLENAAKAIANIAAAGRPVLFVGTKKQVREILVEEAERANSFYVANRWLGGMLT
                     NFQTIRQSIKRLDDLDAKAQDGTFQKLTKNEVLGLEKERQKLNSVLCGIRHMGRLPGA
                     LVVVDTKKERIAVAEANKLGIPVFAVVDTNCDPDLITYPIPGNDDAIRSVGLVLHVLA
                     DAVIEGMQNIQQPEQAEPAAVSRESGERERSRRGGPRRPRGARSGSEAVAA"
     gene            complement(4466..4858)
                     /gene="rpsI"
                     /locus_tag="PLT86_11335"
     CDS             complement(4466..4858)
                     /gene="rpsI"
                     /locus_tag="PLT86_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT37245.1"
                     /translation="MESSVKSMTGRRKEATAMVRMMPGSGQITVNGRPVAEYFARPTL
                     VMMIEQPLKLTDTLGSLDISANCKGGGLSGQAGALRLGIARALVNENPDLRPTLKAAG
                     LLTRDPRMVERKKYGQPGARKRFQFSKR"
     gene            complement(4906..5346)
                     /gene="rplM"
                     /locus_tag="PLT86_11340"
     CDS             complement(4906..5346)
                     /gene="rplM"
                     /locus_tag="PLT86_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT37246.1"
                     /translation="MATYGKGTYTESSKNIEPVWYVVDAENEVLGRLASRIAKVLRGK
                     HRVTYSPHMDLGDSVIVVNAEKVKVTGNKRNSMAYFHYTGYPGGGRIVPFEKMMQKDP
                     TEAVRHAVRGMLPKGALGFSLLGKLRVYAGSEHPHVAQNPQPLP"
     gene            complement(5516..>6582)
                     /gene="carA"
                     /locus_tag="PLT86_11345"
     CDS             complement(5516..>6582)
                     /gene="carA"
                     /locus_tag="PLT86_11345"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOT37247.1"
                     /translation="VVFNTSMAGYQEVLTDPSYARQIVTMTYPLIGNYGVSTADLESS
                     HPWVDGFIVKEYCRWPSNWRSELPLGDWLAQHGVIAMEGVDTRSLTKRLRVKGSLRGC
                     LAAGVAADPHELVEKAREWPGMAGLDCVPLVSRKDSCEWTTGLWDVRNSAEIAPPPPK
                     HHVVAYDFGCKQNILRLLVHHGCRVTLVPGITPPEAVLDLKPDGVFLSNGPGDPEAVT
                     YAISAVKRFAHESEATGLPVFGICLGHQILGWALGGKTFKLKFGHRGGNQPVKDLTTG
                     RVEITSQNHGFAVDPDSLGPEVEVTHINLNDNTVEGLRHRRLPVFGVQYHPEASPGPH
                     DARYLFARFTGLMEARRPDR"
BASE COUNT         1234 a         2006 c         1819 g         1523 t
ORIGIN      
        1 gccgcatcct caccgttgcc tctgtcgcgg gttttcaggc catgccgtac atggcggaat
       61 acgccgccac caaggcatac gtgctttctc tttcggaagc cctgcgagtg gaactcgctc
      121 cgtacggagt cgtggtggcg tgccttgcac ccggaccgac tgatacggat ttcttctctt
      181 cggcgggaac cactatgctg aatacgccgg gaaggaaaac cccggaagag gtcgcccgcg
      241 ttggattcac ggcaatgctc cgagggaagc cgttggccgt tccgggcgcg atgaacaaac
      301 tcatggtgtt cgcgggcagg cttgtgcccc gcacattgtt cgcatgtgcc gccgccaggg
      361 ttctgcggag gaggtaacgt actttgcgtg cggcgctact gaaagagacg cggagcggga
      421 ccgcatcgtg atcccgctcc gcatattgcc ttcgtagagt gtcaacggac gagtgaggct
      481 ttgctcctcg tatactggag ttcgtattcc cgctcccgcc cctcaatgag atcgcgggcg
      541 atcgtaatat cctctttcgc ctccccgggg cccagaaatc cgatagcgac ggtgtcagtc
      601 ggcttgttgc tgccgaatac gaaactcaac cccgtcgggg gcatcacccg acctgcgcca
      661 agcggtttga tgcatatcac cggcttcggt gtctctcgaa tcacccgccc cgtccagtcc
      721 gtctcgaccg gacacagaaa cccgatgctg ttgtagggaa ggatgtacgt ggcgacgtcg
      781 tacccggcct tatccgatac gatgatggtc tccggtcggt gggtgctcca cccgggaatc
      841 atgccgttgt ccctgataca ctggaggatt tccggtccgc cagcgatccg gttttctgct
      901 ggaatgagat tgttgtcagt aaacccctgg tgggggaggc agatttcgac accgtggtcc
      961 gcgcaccatt ttacccccgg ttccacctct ttccagtcgt ttccgcccgg agtgcacatc
     1021 cacacccagt ggtggccggt ctgccgttca tgctcacgaa ggatttcgtg gtagaaggga
     1081 ttcgggctgc tgatcgttcc gttgatcccc tccagacggc acgcttcgag gacctcgagg
     1141 atgcgttcct tcgtgaaatg gttgcgcagc cacgcgtctc tcgccgcgct gaagtgcgaa
     1201 aacccgaaga aagggttcgt accgcaaaga acacgcgaga cctgaacatg ttcaatactc
     1261 gtcatcggga acggcatggt tgtatctccg gtagtgtcga gggcggacaa ttgccaggtg
     1321 aagagtagcg cgagcagtat tcagggacaa acgggagtgc ggtttcaaaa aaggcgcgcg
     1381 gtatcccccg cgcgccttca cgtaacgttt ctatggtgtc ggctcacact tccatgatct
     1441 ctttctcttt tgcggcaagc gcggcatcaa ctttcttgat gaattcgtcc gtcagagtct
     1501 ggatttcctt ctgatcgcgc cggctgtcat cctccgaaac ctgtttcgcc ttctccattt
     1561 ttctcagttc ttcaatcgcg tcacgacgct cgttgcggat tacgatccgt ccgtcctccg
     1621 ccattttgtg cacgatcttc acgtattcct tgcgccgttc ctcggtgaga ggtggtagcg
     1681 caattcgtat gacattccca tcattcgtgg gattcaaacc cagatcggaa agcctgatgg
     1741 ctttgtctat cgcaccaatg gtggacttgt cccacggctg aaccagcagc atcgcaggtt
     1801 cgggagccgt gacattcgcc acttgcgaga tcggaacctt cgacccgtat gcttccactc
     1861 gcacaacatc cagaagcgcg ggattggctt tgccggttct gacagtactg aactcgtgaa
     1921 cgatcgtttc gagagttttc tgcatcttcg cccgcgtctg gcggacgatc tggctttctg
     1981 ttgccatctg cacctccctt gttccggttc gcctacgcca ctactctcgt gccgacacgg
     2041 tcgcccaatg ctgcccgcac gagttcgccg gcgcgggtga ggttgaacac gactatcggc
     2101 agcctgttgt ccatggaaag actgactgcc gtggcatcca tgaccttata accgtgttgg
     2161 agaatatcct cgtacgtgat ttcatcgaaa aggttggcac ccgggtttct ggcgggatca
     2221 tcatcataca cgccatccac cttcgtgcct ttcagcagaa cctccgcgcc gatttccacg
     2281 gcgcgcaacg ccgcagccgt atccgtggta aagaaaggac tgcccgttcc cccggcaaac
     2341 agcaccacgc gctctttttc aaggtgccgg atggcgcgcc ggcgaatgag cggctccgcc
     2401 acctggtcca tacggatggc cgtctgaacg cgcgtttcca gaccgagttt ctccagcgcg
     2461 gcttgcagcg caaggcagtt gatcaccgtt gcaagcatcc ccatggtgtc ggcactggcc
     2521 gcctcaactc caagctggtt gacgatttcc ttcccgcgga agatattccc tcctcccaca
     2581 acaaggccga tctggacccc gcgggagtga acctccacga tctcctgaca gatgcggctg
     2641 acctccacag ggtcaatacc gaacgcgtga gatccctgca gagcctcgcc agacagcttc
     2701 agcagtatcc gcttgaacgc cgacatgggg acttccgtcg ttggaatgtg gaaaaccggt
     2761 gacgcggaac gtcaaagccg ctcattcgcc cactttgaaa cgcacgaacc gcttgatcgt
     2821 gatgttctcg cgaatcttcg caatggcttc cgtcaccaga tccgacacct tgcgggagtc
     2881 atcccgaatg tacgcctgat cgagcaggca cacttcctga aaatacttct cgagccgccc
     2941 ttctacgatc ttgtccagaa tcttctccgg cttcccttcc gctttcgcct gggcgcggga
     3001 tacttctttc tcgtggtcga gaacttcctg aggaacctga tcccggctta catactgggg
     3061 agcagacgcc gcgacctgca ttgcgatgtt ctttcccagc gccttgaagt catctgtgcg
     3121 ggcgacgaaa tcagtttcgc agttgatctc caccagcaca ccaagttgat cgccgggatg
     3181 aacataactg aatatcgcgc cctgacttgt agcgcgtgac cccagctttg cgctcttgag
     3241 aatcccctgc tcccggagat agtcaatcgc cttctggagg tcgccgttcg tctcaccgag
     3301 agccttcttg cactccatca tgccggcgcc ggtcttctct cgcagttcct tcacttgtgc
     3361 ggcggtaatt tccattgtgt tcctcgtaac aaagagacgg tacaacaaat gcggcggagg
     3421 gacgtcgtgg ccccttccgc cgttattttc agtgcaaatc aggctgcaac ggcttcagag
     3481 ccagaacggg cgccgcgcgg gcgacgtggt ccgccacggc gggaacgctc tcgctcgccc
     3541 gattcacggc tcaccgccgc cggttcagcc tgttccggct gctggatatt ctgcatccct
     3601 tcgatgactg cgtccgcaag aacatggagc accagcccca cagagcggat cgcatcgtca
     3661 ttcccgggaa tcggataggt aatcaaatca gggtcgcagt tcgtgtcaac caccgcgaat
     3721 acggggatgc ccagtttgtt cgcttcggca acggcgatgc gttccttttt cgtgtcaaca
     3781 accaccagcg cccctggcag acgacccatg tgacgaatgc cgcagagcac cgaattcagc
     3841 ttctggcgct ccttctcaag ccccagcacc tcgttcttcg tcaatttctg aaacgtcccg
     3901 tcctgcgctt ttgcgtcaag gtcatccaac cgcttgatgc tctgccggat cgtctggaaa
     3961 ttcgtcaaca tgccgccaag ccagcgattt gccacgtaga agctgttggc gcgttccgct
     4021 tcttcgacga ggatttcacg aacctgcttc ttcgtgccga caaacagcac cggacgtccc
     4081 gccgccgcaa tgtttgcgat cgccttcgcg gcattctcca gaagcgcgat tgtcttgttc
     4141 aggtccagaa tatggatgcc gttccgttcc atgaagatga accgcttcat cttcgggttc
     4201 cagcggcgcg tctggtgccc gaaatgaacc cctgcttcca gcaaatcctt catttccacg
     4261 cgtgccaaag ccatcctcct tgcccaccct tcaaccgggt gaacctgttg tgcgtcgttt
     4321 gccgccacgt cacgctatcc accccgatcc gcattcagac ggccagagcc gggaaacgct
     4381 cactccccgc aagggagtga cgacgatcgg gagccgtgat cgtgtgaggg atacctgcaa
     4441 ggcaatctgc caaacaggcg attgattacc gtttggagaa ctggaaccgt ttgcgtgcgc
     4501 ccggctggcc gtactttttg cgttccacca tacgtgggtc acgggtcaga agtccggcag
     4561 ccttcagtgt tgggcgcaga tccgggtttt cgttgaccag cgcgcgggca ataccgagcc
     4621 tgagcgctcc tgcctgtccg ctcaacccgc cgcctttgca gttggcgctg atatccaacg
     4681 acccgagagt atccgtgagt ttgagtggtt gctcgatcat catgacgagg gtcgggcgag
     4741 cgaagtattc cgcgacgggt cggccgttga ccgtgatctg gcccgagccc ggcatcatgc
     4801 ggaccatcgc cgtcgcttct ttgcggcggc cggtcatgct tttcacggag ctttccatgt
     4861 cttcgtgtct ttcctatccg gcagacggtt gacgtaacaa gtttgttacg ggagtggttg
     4921 tggattttgc gcgacgtgcg ggtgttcgga gcccgcgtag acgcgaagtt ttccgagcag
     4981 cgagaatccg agtgcgcctt tgggaagcat cccacgaacc gcgtggcgca ctgcttctgt
     5041 cgggtctttc tgcatcattt tctcaaacgg gacaattctt ccgccaccgg ggtagccggt
     5101 atagtggaag tatgccatgc tgttccgttt gttccccgtg actttgacct tctcagcatt
     5161 aacgacgatg acgctgtcgc ccagatccat gtggggcgag taggttaccc tgtgcttgcc
     5221 gcgaagcact ttcgcgattc tgctcgcaag gcgaccgagc acctcgtttt ctgcgtcaac
     5281 gacgtaccac accggttcta tgtttttcga actctcggtg taggtacctt tgccgtacgt
     5341 tgccatgcca tactccacct gcgttatatg ggaaacaccc gccgcagcgg tggttgagac
     5401 aggagagtct cctgcgcccc ccatccagtc tgtggcgttc gcgttggaca ctatgcgggc
     5461 tgtacagatt tataaagtta cagtaatttg cgggtgtttg tcaatagcgg gtcagtcacc
     5521 ggtcgggccg tctggcttcc atgagaccgg tgaatcgtgc aaaaaggtac cgcgcatcgt
     5581 gcggcccggg ggatgcctcc ggatggtact gaaccccgaa gacgggcaac cggcggtggc
     5641 ggagcccttc gaccgtgtta tcgttcagat tgatatgggt gacctcaact tcggggccaa
     5701 gggaatccgg gtcgacggca aagccgtgat tttgagaggt gatttctacc cggccggtcg
     5761 taaggtcctt tacgggctga tttccacccc tgtgaccaaa tttgagtttg aatgttttcc
     5821 ccccgagtgc ccagcccaaa atctgatggc cgaggcaaat gccgaatacc ggtaagcctg
     5881 ttgcttcgct ttcgtgcgca aagcgcttca ctgccgatat tgcgtacgtt actgcttcgg
     5941 ggtcgcccgg accattggaa agaaacaccc catccggttt gagatccagc accgcctcag
     6001 gaggagtgat tccgggaacc agggtgacgc gacatccgtg gtgaaccagc aagcgaagaa
     6061 tgttctgttt gcacccgaaa tcgtacgcga ccacatggtg tttcggcgga ggtggagcta
     6121 tttccgcgct gtttcgcacg tcccataacc ctgtcgtcca ttcgcatgag tctttgcgcg
     6181 acacgagagg aacgcaatcc agccccgcca tgcctggcca ttcccgtgcc ttttccacca
     6241 gttcgtgagg atcggccgcc acaccggctg cgaggcaccc gcgcaacgac cccttcaccc
     6301 ggagcctttt ggtcagcgaa cgcgtgtcaa ctccttccat ggctatgacg ccgtgctggg
     6361 ccagccagtc tccgagtggc aactcgcttc tccagttgga gggccaccgg cagtattcct
     6421 tgacgatgaa gccatccacc cagggatggg aggattcgag gtcagccgta ctgactccgt
     6481 agtttccgat aagcgggtag gtcatcgtca cgatctggcg ggcgtaggag gggtctgtca
     6541 gcacttcctg gtagcctgcc atggaggtgt tgaatacgac tt
//
LOCUS       DAOSOO010000180         6480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12006_length_6480_cov_5.452607, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000180 DAOSOO010000000
VERSION     DAOSOO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12006_length_6480_cov_5.452607"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="PLT86_11350"
     CDS             complement(<1..1985)
                     /locus_tag="PLT86_11350"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOT37248.1"
                     /translation="MAEIGHYSLLIAFFLALYSAIASFAGTRAPELGESAKRAALALF
                     WLLTIAAVILVYLLAVRDLTVEYIARYTSTVLPMSYAISAFWAGNAGSLLFWVWLLSA
                     YAAVVVWRNRKSPSRLVIPALGVMMLIAVFFLGLILFLYRIDPEMTPGMVRDPFYATL
                     QALFGSPEGGLHPACNPFWRLPFRPAEGLGLNPQLQNPGMMFHPPTLYLGYVGMAVPY
                     AFAMAALLTGSLGNEWLSLTRRWTLVAWFFLSIGNILGGWWAYVTLGWGGYWGWDPVE
                     NAAFIPWLLATAFLHSVIMQEKRGMFRTWNMVLVLITFVSTIFGTYLTRSGVISSVHA
                     FARNEAFNIAFLAFMAVACVVSFAFLFVRRDQLVSESPIESVFSREAAFLFNNVVLVG
                     IAFVVLFGTVFPAISSAFTGVQVSMGPAWFNSVLWPLWAMLLVLTGVGPVISWRRARR
                     NEFQRGFAIPSTLGIVVAAGLALAGMRNGYALAFFAICAFVLGTIVQEMLRTFRTRAA
                     TLDSPPARKAMVAALTPNRRFGAYIVHFGVIIVAVGITGSSLFERKTDLVLRQNEVKP
                     FEDQAFRYMSFRQTREPNRDVYAAGVEVTRAGEVRAKVEVVKEDYHAEQMRWTKARIL
                     SSVAEDVYLTLAAFQEDGSAITLSVRRIPLVVWLWVGG"
     gene            complement(2098..2520)
                     /locus_tag="PLT86_11355"
     CDS             complement(2098..2520)
                     /locus_tag="PLT86_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOT37249.1"
                     /translation="MRKPGTKVLISLGLIAAAIAILVVAATMRQGATIHYYTTTSEFL
                     NRSPDYINRPVRVNGKVVPGSLERVVSDGPTDLRFALGDSVHHTLPVHYRGTAVPDAF
                     REGADVVVEGVYLPTGELEARQILAKCPSKYEAAPANR"
     gene            complement(2606..3835)
                     /locus_tag="PLT86_11360"
     CDS             complement(2606..3835)
                     /locus_tag="PLT86_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37250.1"
                     /translation="MMGRDTMRPRRTIVVVAVVTALTNATGIGVLVISPAIRRVLDLR
                     ADAFGLLLSVGLGFGILGSLASGWLADRGYLKASFGAAFGILVASYAVCMLPASYPVY
                     LLALACAGLANSGALTLANVTAARAFPDGSRKALTGIMLAHSIGGVVAAPLWQMLFEV
                     FDTRFPTESALQLVFLAAACVCAAAGALFFAFPLPTQFAGRKTPAAAEPGDRASLWGL
                     PLVVVCAFAALQAGAYNSAFGWIPDFATRTFAPNPFPVSWILSGISLSYVVGRLILLR
                     LPERLPDLRVIFVCASTGALLFGAAFGSTNQYTMGILCVVGGLVLSADWPSIFSHTAR
                     LFPVHAGKAIAMAGASAGVMSFVLPPFMGFIAEQTGSLRAGMMVPPLLFGVLSVSALS
                     WERYLRRARKHPLHTES"
     gene            complement(3832..5046)
                     /locus_tag="PLT86_11365"
     CDS             complement(3832..5046)
                     /locus_tag="PLT86_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37251.1"
                     /translation="MSDTPDYSAPRLVTVLCIVTAMTNMCALTVYIVSPHIRDALQLR
                     ADAFGLLLSVGLGAGIVGSLSAGWLADRGHMRKTFHAALALLAITYATCSFPPSYARY
                     LIAFTCAGMANTALTTLASVGVTRAYPEGSRKPLTWLLIGYSLAGIVGPPFWGYLFQA
                     FTSVVGGARSLQFVFACALGACALAWAILALNPVPARVAGTGTKKEDVQPGDIPLLGL
                     PLVLVCVFAALHASADNAMFVWIPEFTRAHFSPQPFPSSWILSGVAAAYVLGRLVLLS
                     TPDRLRDLTVITAVAGTSAGLFMLAFNSPNQYLMAGLYVLGGVLLSADYPSIMSYTAS
                     MFKRGTGSALAITGAASAGASLLVPPLVGYISEATGSLVTAMMLPPALLLLLALLAFL
                     RKITETRGRAPT"
     gene            5545..>6480
                     /locus_tag="PLT86_11370"
     rRNA            5545..>6480
                     /locus_tag="PLT86_11370"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1504 a         1865 c         2046 g         1065 t
ORIGIN      
        1 ccgccgaccc acagccacac cacgagcggt atccgtcgca cggagagagt gattgcgctt
       61 ccgtcttcct gaaatgcggc caacgtgagg tacacgtcct ccgccacgct ggaaaggatg
      121 cgggctttcg tccagcgcat ctgctccgcg tggtaatcct ctttcacgac ttccaccttc
      181 gcgcgcacct cgcccgcccg tgtgacttca acccccgccg catagacatc gcggtttggc
      241 tcgcgggtct ggcgaaatga catgtagcgg aacgcctggt cctcaaatgg cttcacttcg
      301 ttctgtcgaa gcaccagatc ggtcttgcgc tcgaacagtg aggaaccggt gattccaacc
      361 gcaacaatga tgacgccgaa atgcacgatg tacgcgccaa aacggcgatt cggggtaagc
      421 gccgccacca tcgccttccg ggcgggtggg gaatcgagtg ttgccgcgcg cgtgcggaag
      481 gtccggagca tttcctgcac gatcgtgccc agcacgaacg cacagatcgc gaaaaacgcc
      541 agcgcgtatc cattgcgcat gcctgcaaga gcaaggcctg ccgccaccac aattcccagc
      601 gtggacggga ttgcgaaacc ccgctggaac tcgtttctgc gtgcgcgacg ccaggaaatc
      661 accgggccga cacccgtcag cacgaggagc atcgcccaca gtggccacag cacgctgttg
      721 aaccacgccg gtcccatact gacctgcaca ccggtgaacg cgctgctgat cgccgggaag
      781 acggttccga acaggacgac gaacgcgatg ccgaccagca ccacgttgtt gaaaaggaat
      841 gcggcttcgc gggaaaacac ggattcaatc ggactttccg acacaagctg atcgcgacgg
      901 acgaacagaa aggcgaacga caccacgcat gcaacggcca tgaacgccag gaacgcgatg
      961 ttgaatgctt cgttccgcgc gaaggcgtgc acggaggaga tcaccccgct gcgtgtgagg
     1021 tacgtgccga aaatggtaga aacgaatgtg atcagaacca gcaccatgtt ccacgtccgg
     1081 aacattccac gcttttcctg catgataacc gagtggagga acgccgtggc gagcagccag
     1141 gggatgaatg ccgcgttttc caccggatcc cacccccagt acccacccca cccgagcgtg
     1201 acatacgccc accagccacc caggatattt ccgatggaaa ggaagaacca ggcgaccagc
     1261 gtccaccgtc gtgtcaacga aagccattca ttgccgaggc tcccggtgag aagggccgcc
     1321 atcgcgaagg catacgggac ggccattccc acgtacccaa ggtaaagagt cggaggatgg
     1381 aacatcatcc cggggttctg aagctgagga ttcagcccga gcccttctgc ggggcggaac
     1441 ggcaggcgcc agaagggatt gcatgcggga tggaggcctc cttccggact gccgaaaaga
     1501 gcctgcaggg tagcgtagaa cgggtcgcgc accatgcccg gggtcatctc ggggtcaatc
     1561 ctgtagagga acaggatgag tccgaggaag aacactgcga tcagcatcat gacaccgagt
     1621 gccggaatga caagccgcga tggcgacttc cggttcctcc acaccacaac agccgcgtac
     1681 gcggagagga gccacaccca gaacagcagc gatcctgcgt tgccggccca gaacgctgag
     1741 atggcgtacg acatgggcag gacggtgctc gtgtacctcg cgatgtactc gacggtcagg
     1801 tcccgcacgg caaggagata tacgaggatc accgcggcga tcgtcaacag ccagaacagc
     1861 gcgagcgcag cgcgttttgc ggattcgccg agttccggag cacgagtgcc cgcaaatgag
     1921 gcgatcgcgg agtagagtgc gaggaagaag gctatgagaa gggagtagtg accgatttcc
     1981 gccatacagg tctcgtttca acggggcaag atggtgcttc ggcgatcgcg gcgcagtggt
     2041 ctggtataca gggcagtaaa tgtaccgcgg gaaccttcaa tgtgccgtat cccgatgcta
     2101 cctgttcgca ggtgcagcct cgtacttgga cgggcatttc gcgagaatct gccgcgcttc
     2161 cagctcgccg gtcggcaggt acaccccttc aacaactaca tcagcccctt cccggaacgc
     2221 gtcgggaacc gcggtgcccc ggtaatggac cggcagagtg tggtgcacgg agtcccccag
     2281 cgcgaatctg aggtccgtag gtccatcgct caccactctt tcaagtgaac ccggcaccac
     2341 cttcccgttt acgcgcacgg gtctgttgat gtagtccggt gagcggttca ggaactctga
     2401 ggtggtggtg tagtaatgaa tggtggcgcc ctggcgcatc gtggcggcaa ccaccagaat
     2461 agcgattgcc gcggcgatga gaccgaggct gatcaggacc ttcgtacccg gtttccgcat
     2521 tgtgtatttc ctgctaacgt cattccggcg tgttttggcg caagaaatgt acgaaacggc
     2581 gttgcgtgaa ccggaatccc gcagctcaac tttccgtatg aagcgggtgt ttccgcgcgc
     2641 ggcgcaaata tctttcccag gataacgcag agacggaaag cactccgaaa agcagaggcg
     2701 gcaccatcat cccggccctc aaggatcctg tctgctcggc aatgaacccc atgaacggcg
     2761 gcagcacgaa cgacatgacg cccgctgaag ctcccgccat ggctattgcc tttccggcgt
     2821 gtacagggaa taggcgggca gtatgcgaga aaatcgaggg ccagtccgcg gagagaacca
     2881 ggcctccaac gacgcacaag atccccatgg tgtactggtt ggtgctgccg aacgcggcac
     2941 cgaaaagcag tgctcccgtg ctggcgcaga cgaaaataac cctcaggtcc ggcaaccgct
     3001 ccggaaggcg gagcaggatg agccttccga cgacgtagga aagcgatatc ccggacagga
     3061 tccacgaaac agggaagggg ttcggggcga aggttcgggt ggcgaaatct ggtatccacc
     3121 cgaacgctga attgtaagcc ccggcctgaa gtgccgcgaa cgcgcagacc acaaccagcg
     3181 ggaggcccca aagtgacgcc cgatctcccg gttcggctgc tgcaggcgtc tttctgcctg
     3241 caaactgtgt tggaagagga aacgcgaaaa agagagcgcc cgccgcggcg cacacgcagg
     3301 cggcggcgag gaacacaagc tgaagcgcag attcggtcgg gaaacgggtg tcgaatactt
     3361 caaacagcat ctgccagaga ggggccgcaa ccacgccgcc aatggaatgg gccagcatga
     3421 tcccggtaag agcttttcgt gacccgtccg ggaacgcgcg tgcggccgtg acattggcca
     3481 gggtaagcgc gccggaattc gccaatcctg cgcatgccag cgccaggagg tacacgggat
     3541 acgaagccgg cagcatgcac accgcataac tcgccacgag aatcccgaac gccgcgccga
     3601 aactcgcctt caggtaaccg cgatccgcga gccacccgga tgcgaggctg cccagaattc
     3661 cgaatcccag accgacggaa agcagcaatc cgaatgcatc cgcgcggagg tccagcaccc
     3721 gccgtatggc cggggagatc acgagcacac cgattcccgt cgcgtttgtc agcgcggtaa
     3781 ccaccgccac gacgacgatg gtgcgccgcg gtcgcatggt atcgcggccc atcatgttgg
     3841 tgctcttccc cgtgtctcgg tgattttcct caagaaggcg agcagcgcga gcagcagaag
     3901 aagagccggg ggaagcatca tcgcggttac caacgaaccg gttgcctccg aaatgtatcc
     3961 gacgagagga ggcaccagaa gcgacgcgcc cgcggaagcc gctccggtga tcgcaagcgc
     4021 gctgccggtc cctcgtttga acatggaggc ggtgtacgac atgatcgagg ggtaatccgc
     4081 cgaaagaagc actccaccaa gaacgtacag cccggccatg aggtactgat tcgggctgtt
     4141 gaacgccagc atgaatagac cggcacttgt cccggccaca gcagttatca ccgtcagatc
     4201 ccgcaatctg tcaggggtgg aaagcaggac cagcctgccc agcacatacg cggcggccac
     4261 acccgaaaga atccaggagg agggaaacgg ctgcggggaa aagtgcgcgc gggtgaattc
     4321 cggtatccag acaaacatcg cgttatccgc gcttgcatga agagccgcga agacgcagac
     4381 gagcacgagg ggcagcccga gcaggggaat gtcgccgggt tggacgtctt ccttttttgt
     4441 ccccgttccg gcgacgcggg caggaacagg attcagcgcc agtatggccc aggcgagggc
     4501 acatgcgcca agagcacagg caaacacaaa ttggagggag cgtgcgccac cgaccacgga
     4561 agtgaaggcc tggaagagat atccccagaa aggcggtccg acgatacccg caagggagta
     4621 cccgataagc agccacgtca acggcttgcg tgatccttcc gggtatgcgc gcgtgacgcc
     4681 gacactggca agcgtggtga gcgcggtgtt ggccatgcct gcgcacgtga atgcgataag
     4741 gtaacgcgcg tacgagggag ggaagctgca cgtggcgtac gtgatggcca gaagcgcaag
     4801 cgcagcgtgg aaggtcttcc gcatgtgacc cctgtccgcc agccaccctg cggagagact
     4861 ccccacaatc cccgcgccca gaccgacgga aagcaacagc ccgaacgcgt cggcgcgaag
     4921 ctggagagcg tcgcggatat gcggggaaac gatgtacacg gtgagcgcgc acatgttggt
     4981 catggcagta acgatgcaca gcaccgtgac gagccgcggg gcagagtaat ctggtgtatc
     5041 ggacatgggg acggaacgaa gagggggccg cggatggaac aaaccgcacg acggaacgat
     5101 taagaaggta gtgcagaaaa tccccggtca ccaacaggga gtgttgacga ttcgccggtc
     5161 taccacgcgt tgagttctgc ggcggaatcg gttaccctgc cgggccgcaa ggagcgggga
     5221 gtggctcggg cttgtgcttg acaatgccgg ggcggactgg tatactctct acttgcgtcc
     5281 ctggtgcgaa cgtcggttcg aaggtgagtg ccgggacgaa aatcgagaaa aaattgaccc
     5341 cgggcacttg acaatcgcga aaggggtcta tagctttaga gactgtggtt ttgccggccg
     5401 cgtgtgaatc gccccggcga agcgcagtgc cttgctcttt gaaaatcgaa tagcgtgcat
     5461 cacgccagac aagatgggct cttgtctccg tcgccttcgg gcggcggtga ttatgagttt
     5521 tggaaataca ggaatcaaat atatcctacg gagagtttga tcctggctca gaacgaacgc
     5581 tggcggcgtg cctaagccat gcaagtcgaa cgggttgtcg caagacaatc agtggcgaac
     5641 tggtgagtaa tacgtgggta atctgcccga gagtggggaa tagcccttcc aacgaggggg
     5701 taacaccgca tattatcgcg aatccgcatg gatttgcgat gaaaacaggg gatcgcaaga
     5761 ccttgtgctc tcggatgaac ccgcgcccca ttagctagtt ggtgaggtaa cggctcacca
     5821 aggcgacgat gggtagccgg cctgagaggg tgaccggcca cactgggact gagatacggc
     5881 ccagactcct acgggaggca gcaggcggga atattgggca atggacgaaa gtctgaccca
     5941 gcgacgccgc gtgagggatg aagcttctcg gagcgtaaac ctcaaacgga tagggacgaa
     6001 aagcctatcg gctaatatcc ggtaggcctg acggtacctg ttacgaagct ccggctaact
     6061 ccgtgccagc agccgcggta atacgggggg agcgagcgtt gttcggaatt actgggcgta
     6121 aagggcgtct aggcgggtcg gtcagtcaga agtgaaaact cccggcttaa ctgggagcct
     6181 gcttttgaaa ctgccggtct tgagtgtgga agaggtgcgg ggaattctca gtgtagcggt
     6241 gaaatgcgta gatattggga agaatgccgg tggcgaaggc gccgcactgg tctaacactg
     6301 acgctgaagc gcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccc
     6361 taaacgatgg gcactaggtg tgggaggtat tgacccctct cgtgccgcag ctaacgcatt
     6421 aagtgccccg cctggggagt acggtcgcaa ggctgaaact caaaggaatt gacgggggcc
//
LOCUS       DAOSOO010000181         6453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12090_length_6453_cov_4.664895, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000181 DAOSOO010000000
VERSION     DAOSOO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12090_length_6453_cov_4.664895"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="PLT86_11375"
     CDS             <1..1623
                     /locus_tag="PLT86_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37252.1"
                     /translation="RGNRMDWVKYDPLFRRFVEDTLTSQEKIKWFDTDDMNEVKLEEA
                     GSGWLWGRSNEFLSWVYVHISPGERRKTWGVEHIEDSWGIRDERDRLLWAETSRFWAS
                     CIRENLRKIKGYGQEVSGKDFLVVPNWGELLTFQELAFREDMGHDIEEWAPATDIVFW
                     EDNRDPGRTAEGLHFDYSLEYKLAHAAGVRSGAMAALPGDHGTCALAHGEAVAHGGAF
                     IQRGPEFADLRGDFNRFLSEHRDWFEGHVSAARVGLVLSFQTYRLGGNTHIRDTFRLF
                     QFLNDQQVPFDIIVDTQLPDKALLRSYSLLVCSELAALSDEATAAFEEYVNGGGILFG
                     CGALGTTDEFGAPRSGFLAGTSGENLIRYNRLNDVLPPGQISREDALEFVTNIWRYLN
                     WSVTKWPLINAGDYAVKIERFVDPGAIGARIPGWSNFQVADPHACNGLRVVPYSKVSG
                     RRRELSVHLLNYNVDLTLPAGARSTEIMRDVAISLPVGHGLVLRDARCARPGKEVEIV
                     DACVKADRVMFSVPELGVYALVHLTFDNHPTR"
     gene            1642..2610
                     /locus_tag="PLT86_11380"
     CDS             1642..2610
                     /locus_tag="PLT86_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOT37253.1"
                     /translation="METEIPVVTKNDLVTGLRSVGLSRGQHVVVHSSLSRFGRVEGGA
                     ETVIAALKEIITPEGTVVMPTFTSRIEFFAEALVARCVAQGKDGFHGTVAELYDTVRQ
                     IWLDAGFQIVPFDTPAALWKRFHDEGVFTHWKNWEISPQIFPLPDDEFVHIVKHGRPL
                     PPDQLHPWCIAGTVGIIPETFRKQPDVRRSQHYSGSFAAWGSMTDFVLKGHDNYSPRV
                     FEDHPLYRMKEIGGKILLLGINHGNNSTIHVAEEAAVRAKGVKVPHEYLGHFMNIEAP
                     LDAAGGQTHGKIGNAEVRLADTRILYRVCAEILDAKLAGGELVPVG"
     gene            complement(2632..4476)
                     /locus_tag="PLT86_11385"
     CDS             complement(2632..4476)
                     /locus_tag="PLT86_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37254.1"
                     /translation="MKSLSRVVPYLLIPAVVLFSCAPQPAVLTAGQQPPVAPAPRTYS
                     YAPDPYPERVKTLVFTTIAPEFLADSAGYFARNGIDGFMVADIMHSWDSDIWKQPTTF
                     TPDEPAGRVVGEGNPLFQICKRMNEQCRAAGMNSNSIKVAFYRPLPDWFDDAAWDSLC
                     ENFRQCAIFARDAGFAGVALDIEYVYPTYELTYEAYLREGYPRDRLPDQAHQRGYEIM
                     SAMISEFPDMVNWHLPETVWTYGPLGVQLFAGMVDALAEVDAPGGIHMCVENMYDVTD
                     PERILNNTLSIDLAVRKALNQSRDKRAFEYWMRRGSIATGMWPLGYYRMVMDASGRFV
                     GYSGKTETFGDRIVGSYADKSANFPPEEFRRQYAAVRQISREYAWIYGHGPVLWQMSE
                     TAMRRYHAAYNDTLPTDVNLEAYLAVLREKPVITDSAFATRAELVRNRFPVVFPGTPP
                     RLWHIGPFPCRKNEFSVVYPPERGVDLAAVYSAYDGYDVPGGALRWKRVDLDATGFVD
                     LKEIVSGQDSVLAYSVAWVDVPTLTRAELRVGSNDYVAVFVNGREVFGSTRGRVALRD
                     ENVIPVQLTPGWSEIMVKCGNVRRAWGFYLRVTDENGNEVPGLRWVEP"
     gene            4629..6185
                     /locus_tag="PLT86_11390"
     CDS             4629..6185
                     /locus_tag="PLT86_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37255.1"
                     /translation="MPQTPEWAKAQTSARRVLERWISSSIARNLEIPYHGIHDEGSFT
                     ASWDAFYFTTRDARIRDFLVWLRDGFADWTKDNLLHGYYPEGEVHHATEPFTHFIARF
                     RTLVPADPLTAHMLEDAAEHVGNWVPEIPAWYDWKTHCMKSWRIGTRVVKTTPPDDYE
                     EPDSVRPAIIALAAYAVTGKERYLAFCCDYADKWAAALLETPLPRVRFLQSAEDLYND
                     RIVHQATGNLQLRLELVVASGLADWLMDLFYLTEKPTYAEASRVVMAGLVPVLADPRN
                     SVAAALVAKYRRVTGDRSLDETIIASLGEPPRYDKAGIVLREDWTDSKETRKGKSMLL
                     NKRIGHRFDQVRWADEEGQEVTEPTGAAWALAWQITGEERYAARAMFLAGERLRLALE
                     KLHDGRDHGCGGNTVGAVASGHGRADRFGHVNSVWGPLVIGSSRVFSAEQPLVIYPYG
                     LPDGVASLVNSAGHAGVEWFNTGEVARTVTWVDGSRPDATPQHVIIQPGERREAPLEK
                     TFPISRAGAG"
     gene            complement(6222..>6453)
                     /locus_tag="PLT86_11395"
     CDS             complement(6222..>6453)
                     /locus_tag="PLT86_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37256.1"
                     /translation="KRLRKGYSMENRDVTDRDRALAQKCVDCPVCSHARKKQRGIAFW
                     FVKHVEGGICPSCRAYERVYGRKAYEPVPPGE"
BASE COUNT         1360 a         1876 c         1885 g         1332 t
ORIGIN      
        1 cgcggaaacc ggatggactg ggtcaagtac gaccctctgt ttcgcaggtt cgtggaggac
       61 actctcacct cccaggagaa aatcaagtgg tttgatacgg acgacatgaa cgaagtcaag
      121 ctcgaggagg cagggagcgg ttggctgtgg ggacgcagca acgaattcct gagctgggtc
      181 tacgttcaca tttcaccggg agaacgccga aagacgtggg gcgtggagca catcgaggac
      241 tcgtggggca tccgcgacga gcgtgaccgg ctcctgtggg ccgaaacgtc acgtttctgg
      301 gcatcgtgca tccgcgagaa cctgcgaaag ataaaggggt acgggcaaga ggtctccgga
      361 aaggatttcc ttgttgtacc caactgggga gagttgctga ccttccagga actcgccttc
      421 cgagaagaca tggggcatga tattgaagag tgggcgcctg caacagacat tgtgttctgg
      481 gaggacaacc gggatccggg cagaacggct gaaggcctcc attttgacta cagccttgag
      541 tacaaactgg cgcacgcggc tggtgtccga agcggcgcaa tggcggcgtt gcccggcgac
      601 cacggtacct gcgccctcgc gcatggtgaa gccgtcgccc atggtggggc gtttattcag
      661 cgcggtcccg aattcgccga tctgagagga gatttcaacc ggtttctttc ggagcatcga
      721 gactggtttg agggacatgt gtcggccgcg cgggtaggtc tcgtcctttc attccagacg
      781 taccgtctgg ggggaaatac gcacattcgc gacacgttcc gcctctttca gttcctcaac
      841 gaccagcagg tgccttttga catcatcgtg gatacccagc ttcccgacaa agcgctgctt
      901 cgcagctact cgctgcttgt gtgttcggag cttgcggctt tgagcgacga agcgactgca
      961 gcttttgaag aatacgtcaa tggcggcggg attcttttcg gttgcggagc cctcggcacc
     1021 accgacgaat tcggggcacc gagatcggga tttctcgccg ggacatctgg agagaacctg
     1081 atacgttaca accgtttgaa tgatgtgctc ccaccgggac agatttcgcg agaggacgct
     1141 ctggaattcg ttacgaacat ctggcgatat ctgaactggt cagttacaaa atggcccctg
     1201 atcaacgccg gagactatgc ggtcaaaatc gaacgattcg ttgatcccgg agctattgga
     1261 gcgcgcattc cgggatggtc gaatttccag gttgccgatc cccacgcgtg caatggtctg
     1321 agggtggttc cgtattcgaa ggtcagcgga cgccgccgtg agctgagtgt ccatctgctc
     1381 aattacaacg tcgatctgac cttgcccgcg ggagcaaggt ccacagagat catgcgggac
     1441 gtcgctattt cgcttccggt cggacacgga cttgttcttc gagatgcccg ctgcgctcgg
     1501 cctggcaaag aagtggagat cgtcgacgcg tgcgtcaagg ctgatcgagt gatgttcagt
     1561 gtgcctgaac tcggtgttta cgcgttggta cacctcactt tcgataacca ccctacacgc
     1621 tgaattcctg agggaacacg gatggaaaca gaaatccccg tcgtaacgaa aaacgatttg
     1681 gtcaccggtc tgcgctcagt tggtctttcg agaggccagc atgttgtcgt ccacagctcc
     1741 cttagccggt tcggcagggt ggaaggcggt gccgaaaccg tgattgcggc gctcaaagag
     1801 attatcacgc ctgaagggac cgtggtcatg cctaccttca caagccgtat cgaattcttc
     1861 gccgaagctc tcgtcgcccg ctgtgtggct cagggcaagg atggctttca tggcacagta
     1921 gcggaacttt acgacaccgt tcggcagatc tggttggatg cgggctttca aattgttccg
     1981 ttcgatacgc ctgcggccct ctggaaacgc ttccatgacg aaggtgtgtt cactcactgg
     2041 aaaaactggg agatttcccc ccagattttt cccctgccgg acgacgagtt cgtgcacatc
     2101 gtgaagcacg gtcgaccgtt gccaccggat caattgcacc cgtggtgcat cgccggaacg
     2161 gtcggcatca tccctgagac gttcaggaaa caacccgatg tgcgcaggag tcagcattat
     2221 tcaggatcgt tcgcagcgtg gggctccatg acggatttcg tcctcaaagg gcacgacaac
     2281 tactcgccga gggtgttcga agaccatccc ttataccgga tgaaggaaat cgggggaaaa
     2341 atccttcttc tgggaataaa ccacggcaac aactccacaa ttcacgtcgc cgaagaagct
     2401 gcagtgagag cgaagggcgt caaagttccc cacgaatacc tcgggcactt catgaacatt
     2461 gaagcacctc ttgatgcggc aggcgggcag acacacggga agataggaaa tgcggaggtg
     2521 cgactggcgg acacccgcat cctttaccgc gtctgcgccg agatactgga tgcgaaactt
     2581 gcgggtgggg aactggttcc tgtcggataa atgattcgat gcgttccggc gctacggctc
     2641 cacccatcgc aaacccggga cctcattgcc gttttcgtcg gttacgcgaa ggtagaagcc
     2701 ccaggccctc cggacgttac cacacttgac catgatctcg ctccaaccgg gagtaagttg
     2761 gacggggatg acgttttcgt cgcgcagtgc aacccgcccg cgggtcgagc cgaacacctc
     2821 ccttccgttc acgaacaccg caacatagtc gttgctcccg acccgcaact ctgcgcgggt
     2881 caacgtcggg acgtccaccc acgcgacgct gtacgcgagc acgctgtcct gcccgctcac
     2941 gatttccttc agatcaacga atcccgtcgc gtccagatcc acccgtttcc aacgcagggc
     3001 tcctcccggc acgtcgtacc catcgtacgc cgagtacacg gcggccagat caacacctct
     3061 ttcgggaggg tacacgaccg agaattcgtt cttccggcag gggaaaggtc cgatatgcca
     3121 caatcgcggc ggagtcccgg ggaacaccac agggaagcga ttccgcacga gctcggcgcg
     3181 cgtggcgaac gccgagtcgg tgatcacggg tttctcccgc agaactgcca ggtacgcctc
     3241 gaggttgaca tccgtcggca gagtatcatt gtatgctgcg tggtaccttc tcattgcggt
     3301 ctcgctcatc tgccacagaa ccggcccatg cccgtaaatc catgcgtact cacggcttat
     3361 ctgtcgcaca gccgcgtact gcctgcggaa ttcctccggc gggaaattgg cgctcttgtc
     3421 ggcgtacgat cccacgatcc gatctccgaa ggtctccgtt ttgcctgagt aacccacaaa
     3481 ccgcccggac gcatccatga ccatcctgta gtacccgagc ggccacattc ccgttgcgat
     3541 cgagccgcgt cgcatccagt attcgaacgc gcgtttatct ctcgactggt tcaacgcttt
     3601 ccgaaccgcc agatcaatgg acaaagtgtt gttcagtatc cgctccgggt cggtaacgtc
     3661 atacatgttc tcgacacaca tgtggatgcc ccccggtgcg tcaacctccg ccaaagcatc
     3721 taccatgccg gcaaaaagct ggacacccag cgggccgtac gtccacaccg tttccggcag
     3781 atgccagttc accatgtcag ggaattccga aatcatcgct gacatgattt cgtatccccg
     3841 ttgatgcgcc tggtccggca atcgatcgcg agggtatcct tcccgaaggt atgcctcata
     3901 agtcagttcg tatgtgggat agacgtactc gatatccaga gccactccgg cgaaaccggc
     3961 gtctcgcgcg aagattgcgc actgacggaa gttctcgcag aggctgtccc atgccgcgtc
     4021 atcgaaccag tcgggtagag gacgatagaa cgcaaccttg atgctgttgg aattcattcc
     4081 cgccgctctg cactgctcgt tcatgcgctt gcagatctgg aacaacgggt taccctctcc
     4141 gaccacacgg cccgcgggct catcgggcgt gaacgttgtc ggttgtttcc aaatgtcgct
     4201 atcccagctg tgcatgatgt cggcgaccat gaacccgtcg atcccgttcc ttgcgaaata
     4261 cccagcgctg tcagcaagga attccggtgc aatcgtcgtg aacacgagtg tcttcacgcg
     4321 ctcggggtat ggatcgggag catacgagta tgttcttggt gccggagcca ccgggggttg
     4381 ctggccggct gtcaacacag ccggttgcgg tgcgcaggaa aaaagaacaa cggcgggaat
     4441 cagcaggtaa ggaacaacgc gactaagcga tttcacgacc acctcttgcg gcaaatgaga
     4501 ttatgcatta accatacaac ggaacggctc ttccgtccaa cgcggccgcg aacttgccgc
     4561 ttatgtcaaa cagcgtcccg aactttggga cgcacgccgg gtaccgcatc ctgcgaaggg
     4621 aaatctctat gccacagact cctgaatggg caaaggccca gaccagcgcc cgacgtgttc
     4681 tggaacgctg gatttcgtct tccatcgcga ggaaccttga aatcccgtac cacggcatcc
     4741 acgacgaagg gagtttcacg gcctcgtggg acgcgtttta cttcaccacg cgggatgccc
     4801 gcattcgcga ctttctggtg tggctccgcg acggatttgc ggattggacg aaggataatc
     4861 tcctgcacgg gtattacccc gaaggcgagg ttcaccacgc caccgagccg tttacgcatt
     4921 tcatcgcacg gttccggacg cttgtgccgg ctgacccgct gaccgcccat atgctggaag
     4981 atgctgccga gcacgtggga aactgggtgc ctgagattcc agcctggtac gactggaaaa
     5041 cccactgcat gaagagctgg cgcatcggta cgcgggtggt caaaaccact ccccctgacg
     5101 attacgagga accggattct gttcgacccg cgatcatcgc gctggcagca tacgcggtta
     5161 cgggaaagga acgctatctc gcgttttgct gtgattacgc cgataagtgg gctgcggcac
     5221 ttctggaaac gcctcttccc cgcgtgcgat ttcttcagag cgccgaggac ctgtacaacg
     5281 acaggatcgt ccatcaggct acaggaaatc tgcaactgcg cctggaactt gtggtggcgt
     5341 cagggcttgc agactggctc atggatcttt tctacctcac agagaagccc acctacgcgg
     5401 aggcttcgcg agtcgtcatg gccggtctcg tgccggttct ggcagatccc cgcaactcgg
     5461 ttgccgcggc tcttgtcgca aaataccgtc gggtaacagg agacagaagc ctcgacgaaa
     5521 ccattattgc gtcgctcgga gaacccccac gctacgacaa agcaggaatc gttctgcgcg
     5581 aagattggac ggactccaag gaaacccgca aagggaagtc gatgctgctg aacaaacgca
     5641 tcggacacag gtttgaccag gtcaggtggg cagacgaaga agggcaggaa gtcaccgaac
     5701 cgacaggcgc cgcgtgggct ctcgcgtggc agatcactgg cgaagaacga tacgccgcga
     5761 gagcgatgtt cctagcgggt gaacggctgc ggctcgcatt ggagaaactc cacgacgggc
     5821 gcgaccatgg gtgcggaggg aacaccgtcg gcgcggtcgc ttccgggcac gggagagccg
     5881 atcggttcgg acatgtgaac tcagtctggg gacctctggt gatcggatcg tccagggtgt
     5941 tttctgctga acagccgctg gtgatttacc cttacggact gcctgatgga gttgcatccc
     6001 tggtcaactc ggcgggccac gccggagtgg aatggttcaa tacgggagag gttgcccgta
     6061 ctgtcacgtg ggtagatggc tcaaggcctg acgccactcc gcaacacgtg ataatccagc
     6121 ccggagagcg acgggaggct ccactggaga aaacattccc gatctcacgt gccggtgcgg
     6181 gatgacggct cccggcggga cctgaccatg gtctgtcggg atcactcccc cggcggaacc
     6241 ggttcgtacg ctttgcggcc gtatacgcgt tcgtaggctc tgcacgacgg gcagattccg
     6301 ccttcgacgt gtttgacgaa ccagaatgcg atgcctcgct gcttttttct cgcgtggctg
     6361 cataccggac aatcaacgca cttctgagcg agagcgcggt ccctgtcggt aacgtcccgg
     6421 ttttccatgc tgtatccctt tctaaggcgt ttc
//
LOCUS       DAOSOO010000182         6386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12270_length_6386_cov_5.460433, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000182 DAOSOO010000000
VERSION     DAOSOO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12270_length_6386_cov_5.460433"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLT86_11400"
     CDS             complement(<1..501)
                     /locus_tag="PLT86_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT37257.1"
                     /translation="MSTVLAKKVQSGYACALNNERMGCAAPRRPAFGREPAAGGLRDV
                     QDRLRALRGTLGYRRFAARVGAKPNSVRRYELGMARVPLDYVQAVCRAFNVSADWLLF
                     GIEAPAGLADAAVRAIREGRLPATRSAIVAGERVRIVCVPVLNRVPAGAPAEMLDDMP
                     VGFGLEG"
     gene            607..1383
                     /locus_tag="PLT86_11405"
     CDS             607..1383
                     /locus_tag="PLT86_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37258.1"
                     /translation="MTRHPVDPLETHEHECPELPGVCRDILTAPMALDRLLRPDFPAR
                     GEMLDEPCADAEAEPDSIEAVLSSAEADFEWKEPASDSAGAGRGRWGDLSRIRRIPLA
                     DYRPREVVPDEVLTRQNADRGRPHPIRDRPAPEPDTEDEPRGPGRVWELRDRLRECAM
                     CGGLFIPSRFNHRFCSRWCAWKSRVHVCVCPACGVEFVAERPEQVWCSVACGASSRRT
                     TEYDHICARCGNPYRATRATQRFCSRACARASRKRTRRAG"
     gene            complement(1540..2259)
                     /locus_tag="PLT86_11410"
     CDS             complement(1540..2259)
                     /locus_tag="PLT86_11410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37259.1"
                     /translation="MPAPWYEQFFGHDYARFDRHPDTGREIAFLQQVLPVPPARVLDL
                     ACGAGRHAAPMSRAGYDVVGLDLSPVLLSRARKKRSTAQWLRADMSRIPLRDGSCDAV
                     ISLFSSIGYFDDESDNYRVLSEAARVLRKGGRLVIETVNRDFFIRHAPPQSWFWQGAL
                     TVLEERRMDPLTSRSEVDVVVLEGGRHRVYHHSIRLYTAAELATLLASAGVEVLDVFG
                     DYDGAALSFDTARMVVVGERR"
     assembly_gap    2329..2428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2509..2664
                     /locus_tag="PLT86_11415"
     CDS             2509..2664
                     /locus_tag="PLT86_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37260.1"
                     /translation="MRTSLIPSLAAAAASHIRRTYAVSARDQIVNSLVTILLLVLVLG
                     LLVPVSR"
     gene            3014..4705
                     /gene="ilvB"
                     /locus_tag="PLT86_11420"
     CDS             3014..4705
                     /gene="ilvB"
                     /locus_tag="PLT86_11420"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOT37261.1"
                     /translation="MEGKECTGAEILVECIIREGVDLMFGYPGGVVLKVFDELYARRD
                     QIRVVLPRHEQAGVHAADGWAKVTGKVGTVFVTSGPGATNAVTGIATAQMDSVPLVVF
                     TGQVSRELIGSDAFQETDAIGVTRPIVKHSYMIERPQDIARIVRQAYYIASTGRPGVV
                     LVDVPKNVTFEKAVFEWPETVSIRSYKPTTEGHPKSIQRLADAIAESKRPYLYVGGGV
                     VMANASDHLLELVNKTNIPVTTTLMALGAFPGTHPLFAGMPGMHGSKLANICFQECDL
                     VIAVGARFDDRVTGKLSAFAPKAKIAHVDIDPSSIKKTVKVDYPVVGDAHWVLRELNK
                     VVKPRQPNEWNRTFAEMKETHWFRYRPSTEVMRPQYVIEKIYEMTKGDAIISTEVGQI
                     QMWAAQFYKFDKPRRWLTSGGLGTMGYGFPAAIGAQFAAPDAMVIDIAGDGSIQMNIQ
                     ELTTVVENRLPVKVVILNNGYLGMVRQWQEFFYDRHYSSSVIGGLPDFAKVADAFGAK
                     GLTARTHDDVVPVLEEGLAHPGPVFMDFHINPEENVFPMVPAGKALDEMQEEEWA"
     gene            4706..5230
                     /gene="ilvN"
                     /locus_tag="PLT86_11425"
     CDS             4706..5230
                     /gene="ilvN"
                     /locus_tag="PLT86_11425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT37262.1"
                     /translation="MNRRHIISVLVENHFGVLARISSLIAGRGFNIDSLTVGETEDPT
                     VSRMTIVTRGDEQIIEQITKQLNRLIDVIKVTDMTECAHVERELVLIKVAAPTAQTRT
                     EVVQVVDVFRARVVDMSTATLTIEITGDSEKVDAFIEMIEPFGIKEMARTGSVAMGRE
                     RNGRASRGAETKVA"
     gene            5332..>6386
                     /gene="ilvC"
                     /locus_tag="PLT86_11430"
     CDS             5332..>6386
                     /gene="ilvC"
                     /locus_tag="PLT86_11430"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT37263.1"
                     /translation="MSTQKEETTALATETRARMYYDDDANLDLLKDKTVAVIGCGSQG
                     HAHALNLQDSGVKVVVGLRQGSKTWAKAEAAGLKVLETAEAAKQGDIIMMLVPDTAAS
                     EIYERYIAANLTPGKALAFAHGFNIHFKTIVPPTNVDVFMVAPKGPGHLVRATYTRGS
                     GVPNLIAIYQNPSGKAKDIALAYAKGIGGTRVGVIETTFKEETETDLFGEQVVLCGGL
                     SKLIQTAFETLVEAGYQPEIAYFECLHEVKLITDLIYVGGISTMRYSISDTAEWGDYV
                     TGKRIITEETRNEMRRVLAEIQSGKFAKEWVAEYKAGLPNFKRMREEQSQHLIEQTGA
                     PLREMMKGALKLEGGGT"
BASE COUNT         1258 a         1953 c         2006 g         1069 t
ORIGIN      
        1 cccctccagc ccgaacccca ccggcatgtc gtccagcatc tccgccggag cgcccgccgg
       61 cacgcggttc agcaccggca cgcagacgat ccgcacgcgc tcccccgcaa cgatcgcgct
      121 ccgcgtcgcg ggcagacgcc cctcccggat cgcccgcacc gccgcgtccg ccaaacccgc
      181 cggcgcctcg atgccgaaca gcagccagtc cgccgatacg ttgaacgccc tgcacacggc
      241 ctgaacgtaa tccagcggca cccgcgccat ccccagctca taccggcgca ccgagttcgg
      301 tttcgccccc acccgcgcgg cgaaccgccg gtatcccagc gtgccccgca gcgcccgcag
      361 gcggtcctgc acatcccgga ggccgcccgc cgctggttca cggccgaacg ccggtcttcg
      421 aggggcggcg cagcccatcc gctcgttgtt cagggcgcag gcgtaccccg attgaacttt
      481 tttcgccaaa actgttgaca cgaagacgcc tcctttccag tgttgatcat acacgtaaat
      541 atgcgggtag ttctgcccgg acaaaagtgc catgaccagg tgacattgtg aaaggagaaa
      601 acctgaatga cgcgccaccc cgttgatccg ctggaaacgc acgaacacga atgccccgaa
      661 ctgccggggg tctgccgcga tatccttacc gcccccatgg cgctggaccg gttgctgcgg
      721 cccgattttc cggcgcgggg ggaaatgctg gacgaaccct gcgcggatgc ggaggcagag
      781 ccggattcca tcgaggcggt cctctcgtcg gccgaggcgg atttcgagtg gaaggaaccc
      841 gccagcgact ccgcaggggc cgggagaggt cggtgggggg atctctcccg catccggcga
      901 atcccgctcg ccgactatcg ccccagagag gtcgttccgg atgaggttct gacccggcag
      961 aacgcggaca gggggcgccc ccatccgatc cgcgaccgcc ccgcgccgga gccggatacc
     1021 gaagacgaac cgcggggacc cggacgggtc tgggagctgc gcgatcgtct gcgggagtgt
     1081 gcgatgtgtg gaggcctttt catccccagc cggtttaacc atcggttttg ttcgcggtgg
     1141 tgcgcatgga aaagccgggt tcacgtctgc gtctgcccgg cgtgcggcgt cgagttcgtc
     1201 gcagagcggc ctgagcaggt ctggtgttcc gtcgcctgcg gtgcttcctc ccgccgcacc
     1261 acggagtacg accacatctg cgcccggtgc gggaatccgt accgcgccac ccgggccacg
     1321 cagcgtttct gcagccgcgc ctgcgcccgt gcctcccgga aacgcacgcg ccgtgccggg
     1381 tagccgccgg ggagggatac gttccgtcgg gtcctgcgcg ggttatcgag gctgcacccc
     1441 gcgccggtcg tcgagtggcg aaccggtccc tgagcctgct gcccgtgcag gcagcctgtc
     1501 gaagggcggg ttcgttgtat cgagacgacc agccccgggt taccgcctct ccccgaccac
     1561 caccatgcgc gcggtgtcga aggaaagggc cgcgccgtcg taatcaccga acacgtcgag
     1621 cacctccacc cccgcggagg cgagcaacgt cgccagttcc gccgcggtgt agagccggat
     1681 cgagtggtgg tagacgcggt ggcggccgcc ctccagcacc accacatcca cttcggagcg
     1741 gctcgtaagc gggtccatcc gccgctcctc cagcacggtc agggcgccct gccagaacca
     1801 cgactgcggc ggcgcgtgcc ggatgaagaa atcgcggttg acggtttcga tgacgagccg
     1861 gccgcctttt cgcagcaccc gcgccgcctc gctgagtacg cggtagttgt ccgattcgtc
     1921 gtcgaagtag ccaatgctgc tgaacaggga aatcaccgca tcgcacgagc catcgcggag
     1981 ggggatgcgc gacatatccg cgcgaagcca ctgcgcggtc gaacgctttt tacgggcgcg
     2041 ggaaagcagg acgggggaga ggtccagacc gaccacgtcg taccccgcgc gcgacattgg
     2101 agccgcatgc cgtccggcgc cgcacgcaag gtccagcacc cgcgcgggag gcaccggcag
     2161 aacctgttgc aggaacgcga tttcccgccc ggtgtcaggg tgccggtcga accgggcgta
     2221 gtcgtgcccg aagaattgct cgtaccatgg tgccggcatg gcgtggtcct ttcgcgatta
     2281 accgtttgtt gttccgtctc aacggtacca cggtcgtcgg cgcgggggnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ctcttgacat cgcacgggcg cttttcctat
     2461 tttttgaact gactcaagcc ctacacgcac gacttcaggg ttacgaacat gcgcaccagt
     2521 ctgattccat ctctggccgc ggctgcggca tcgcatatac ggcgcacgta tgccgtgtcg
     2581 gcgcgggatc agattgtcaa ctccctcgtc accattctcc tccttgtact cgtactgggg
     2641 ctactcgtac cggtaagccg gtagcgaggc cccgttcgcc gtcagttctc ccctggaaca
     2701 cagacgaatg gagcctcgga tcggcgatcc gcggggaggg atgttgtgtt tttggcgctc
     2761 gcatgatgga aaaagggtat aagtccagtc taacaggcag gatacgcacg atgaaccagt
     2821 caggagcaca cgccggtcgc acgccgcgca caatcacgca cgcgccgttc gccggcagaa
     2881 cggtacggga aaaccattga cccggcacga caatcaggtg aagtcacttc gtcaattgac
     2941 agcaaaggag ccgagaacaa tggctgattc cggtactgcg aaagccgcca acagagcctt
     3001 ctggcggcgc gatatggaag gcaaagagtg caccggcgcg gagatactgg tcgaatgcat
     3061 catccgcgag ggcgtggacc tcatgttcgg ctaccccggc ggtgtggtgc tgaaggtatt
     3121 tgatgagctg tacgcacggc gcgatcagat ccgcgtggtt ctgccgcgtc acgaacaggc
     3181 aggggtgcat gccgccgacg gctgggcgaa ggtgaccggc aaggtcggga cggtgttcgt
     3241 aacgagtggg cccggcgcca cgaacgcggt gaccggaatc gccaccgcgc agatggattc
     3301 cgttccgctg gtggtgttca cgggtcaggt ctcccgggag ttgatcggtt cggacgcgtt
     3361 tcaggaaacg gatgccatcg gtgttactcg gccgatcgtg aagcacagct acatgatcga
     3421 acgcccgcag gatatcgcgc gcatcgttcg gcaggcgtac tacatcgcct ccaccggccg
     3481 ccccggcgtg gtgctggtgg acgtgccgaa gaacgtgacg ttcgaaaagg cagtgttcga
     3541 atggcctgag acggtgagca ttcggagcta caagccgacc accgaggggc atcccaaatc
     3601 catacagcgg ctggcagacg caatcgccga gtcaaagcgg ccgtacctct acgtcggcgg
     3661 cggtgtcgtc atggcgaatg cctccgatca cctgctcgaa ctggtgaaca agaccaatat
     3721 tcccgttacc accacgttga tggccctcgg cgcattcccc ggcacgcatc cgctgttcgc
     3781 cggaatgccc ggaatgcatg gcagtaagct ggcaaacatc tgctttcagg aatgcgacct
     3841 tgtcatcgca gtgggtgctc gcttcgatga ccgggtgacc ggaaaactct cggcgttcgc
     3901 gcccaaggcg aaaatcgccc acgtggacat tgacccttcc tccatcaaaa agacggtgaa
     3961 agtggattac cccgtggtgg gcgacgcaca ctgggtgctg cgcgagctga acaaggtggt
     4021 gaaaccccgc cagcccaacg agtggaaccg tacgttcgcg gaaatgaagg aaacgcactg
     4081 gttccgttac cggccgagca ccgaagtgat gcggcctcag tacgtgatcg agaagatcta
     4141 cgagatgacg aagggcgatg cgatcatctc caccgaggtg gggcagatcc agatgtgggc
     4201 ggctcaattc tacaagttcg ataagccgcg ccggtggctt acctcaggcg ggctcggaac
     4261 catgggatac ggcttccccg cagcaatcgg tgctcaattt gccgcgccgg atgccatggt
     4321 gatcgacatc gcgggtgacg ggtccattca gatgaacatc caggagctca ccacggtggt
     4381 agaaaaccgg ctgccggtga aagtcgtgat cctgaacaac ggatacctcg ggatggtgcg
     4441 tcagtggcag gagtttttct acgaccggca ctactcgagc agcgtgatcg gcgggttgcc
     4501 tgatttcgcg aaagtagcgg atgcgttcgg cgcgaaaggc ctgaccgcgc gcactcatga
     4561 cgacgtcgtg ccggtgctcg aagaagggct ggcgcatccg ggcccggtgt tcatggattt
     4621 ccacatcaac ccggaagaga acgtgttccc gatggttccc gcgggcaagg ctctggacga
     4681 gatgcaggaa gaggagtggg cataaatgaa ccgccgacac atcatcagtg tgcttgtgga
     4741 gaaccatttc ggcgtcctgg cgcgcatcag cagtctgatc gccggacgcg ggttcaacat
     4801 tgacagcctc accgtcgggg aaacggaaga ccccaccgtc tcccgcatga ccatcgtgac
     4861 gcgtggcgac gagcagatca tagagcagat caccaagcag ttgaaccgtc tgattgacgt
     4921 catcaaagtc accgacatga ccgaatgcgc gcatgtcgag cgcgaactgg tgctgatcaa
     4981 agtggcggcg ccgaccgcgc agacgcgcac cgaagtcgtg caggtggtgg atgtgttccg
     5041 cgcccgcgtg gtggatatga gcacggccac gctcaccatc gaaatcaccg gtgattcgga
     5101 aaaagtggat gcgttcatcg agatgattga accgtttggc atcaaggaaa tggcgcgcac
     5161 cggttctgtc gccatggggc gtgaacggaa cggcagagcc agccgtggag cagaaaccaa
     5221 agttgcgtaa attcacaggg ggccaccccg gcacatcggg gcagccctct gtttttcgcc
     5281 ggtcctggtg attgtgcaac ctgagtgttt tcccggaagg attcgtgaca gatgagcacg
     5341 cagaaagaag aaaccaccgc ccttgccacc gaaacccgcg cccgcatgta ctacgacgac
     5401 gacgcgaatc tggatctcct caaagacaaa accgtcgccg tcatcgggtg cggttcgcag
     5461 ggccacgccc acgcgctgaa cttgcaggac tctggcgtga aggtggtggt aggcctgcgg
     5521 caggggtcga aaacgtgggc gaaggccgaa gcagcgggcc tgaaagtgct ggagaccgca
     5581 gaagcggcga aacagggtga catcatcatg atgctggtgc ccgacaccgc cgcatcagag
     5641 atctacgagc gctacatcgc ggcgaacctg acccccggca aggcgctggc gtttgcgcac
     5701 ggcttcaaca tccatttcaa aaccatcgtt ccgcccacaa acgtggacgt attcatggtg
     5761 gcgcccaaag gtccgggcca cctcgttcgc gcgacgtaca cccgtggcag cggcgttccg
     5821 aacctgatcg ccatctacca gaatccgtcc ggcaaggcga aggacattgc gctggcgtat
     5881 gccaagggta tcggcggaac ccgcgtcggg gtgatcgaaa ccacgttcaa ggaagagacg
     5941 gaaaccgatc ttttcggcga gcaggtggtg ctgtgcggtg gtctgtcgaa gctgatccag
     6001 acggctttcg agacgctggt tgaggcgggg tatcagccgg aaatcgccta tttcgagtgc
     6061 ctgcatgagg tgaagctgat taccgacctc atctacgtcg gcggcatctc caccatgcgc
     6121 tactctatct ccgacaccgc cgagtggggc gattacgtga cgggcaagcg catcatcacg
     6181 gaagagacca ggaacgaaat gcgcagggtg ctggcggaaa tccagagcgg caaattcgcg
     6241 aaggaatggg ttgccgagta caaggccggc ctgccgaatt tcaagcgcat gcgggaagaa
     6301 cagtcgcagc atctgattga gcagaccggc gcaccgttgc gcgaaatgat gaagggcgcg
     6361 ctgaaactcg aaggcggcgg cacgga
//
LOCUS       DAOSOO010000183         6315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12450_length_6315_cov_6.853035, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000183 DAOSOO010000000
VERSION     DAOSOO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12450_length_6315_cov_6.853035"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1684)
                     /locus_tag="PLT86_11435"
     CDS             complement(224..1684)
                     /locus_tag="PLT86_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37264.1"
                     /translation="MDALREIGDRLELFVDDWLIDELHGARQLLHQPVPREPALVTDR
                     PWEGNMCHYVTVLRDGALCRMYYSTWHAQFDRNADGSVSLTHTKSNIACAESLDGIHW
                     HRPEYGLFEVDGSRRNNLVWAGEGDEIRGTHGFAPFIDVNPSCPADQRYKAVGRAEKK
                     RDLWAMASPDGIRWRILGDKPILSGLPFDSQNLVFYDALRGEYRAYVRDFDNGVRGIR
                     TATSPDFVNWTEPKWLEYPGAPVQQLYTNQIMPYPRAPHIFLGFPTRYTERKWSPAIE
                     ALPELEHRRLRAGASERYGAAVTDGLFMASRDGQTFHRWNEAFLRPGLRTEGSWTYGD
                     MYQAWGLLETESDIAGAPPEFSLYVVEGYWRGNANLIRRYTLRMDGFVSVNAPFAGGE
                     MVTKPFTFSGSRLTLNMSTSAAGSIRVEMQGANGKPLEGFALDDCVEVIGDELARTVS
                     WKGGPDVSRFAVQPVRLRFVLSDADLYAIQFAGHGG"
     gene            complement(1700..3271)
                     /locus_tag="PLT86_11440"
     CDS             complement(1700..3271)
                     /locus_tag="PLT86_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37265.1"
                     /translation="MSDATHMLLHKAFQIGYVPILGSYTVPTVEEIEKAASMGMNTVI
                     AGQTHLDLAAPEGKACRQRGIRVLYPLEKFVYHGVSLRNEISAEQTEIPLFLAPRHHF
                     SDSLVIALDDELIRYERVENGTLIGSARGWNGTRPARHREGTILFWPEDCEQEVLRVK
                     DSPNLLGWYVLDDSPGDARSALRAMYRIVRKYDPEIRQPVCAGYGDAGSVLNFGPGIC
                     DIMMIYWYPVEPDRYMRESTSEEVQWILAKARERVPGVPFIGVFQSFDGRPMNSGQGL
                     PTSEQVREQMEDFVREGACGLIAFQSSKWGWIDTPELEKPIAKAYREIRQTSGLFVRP
                     ETKEMARRRTQPQGFWTTPNHVPGYVPAWRVAAPFDVPEGKTLDAVFPPDETIDFDAV
                     YEGPFGEIRWRTWETVGGEMEISNLFNRGLIVHKTAYAVSDVENDRERDALMGISSDD
                     DCIVRINGTEVYRYEGSGGVTRDKFKVHVRLPKGRFRLFVKCHNRLGGWGFSLRFMDL
                     AGKPLKGLRFDPERQ"
     gene            complement(3395..3976)
                     /locus_tag="PLT86_11445"
     CDS             complement(3395..3976)
                     /locus_tag="PLT86_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOT37266.1"
                     /translation="MNLESLEQRIARVVREDVAIVPYDPAWPRMFAEEKAHLLASFPD
                     GLIRRIEHFGSTAVPGLAAKPIIDMLVEVRSLEETKTRIAPVLEAQGYDYFWRPLFGD
                     NVPPFYAWFIKRDRAGSRTHHIHMVEADSALWERLLFRDYLIAHPETAKEYEELKLRL
                     AREYPNDRVAYTNGKSAFCIRITRLARERVRPH"
     gene            complement(4164..4598)
                     /locus_tag="PLT86_11450"
     CDS             complement(4164..4598)
                     /locus_tag="PLT86_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT37267.1"
                     /translation="MPTVRLAEGPIIVFLTVCAKDRKMILATREAHQTLCEAWEATRG
                     WLVGKYLIMPDHVHLFCSPAGPDHPELSVWVKCWKTYASIRWPRRSEQPIWQKSFWDT
                     QLRRSERYEDKWDYVVQNPVRKRLCGNPNEWPFQGEINVLPW"
     gene            complement(4739..6019)
                     /locus_tag="PLT86_11455"
     CDS             complement(4739..6019)
                     /locus_tag="PLT86_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37268.1"
                     /translation="MNLLFIFTDQMHRYALRCMGTPDIHTPNLDALAQRGMLFERAYS
                     NCPVCTPFRINLFTGLYSSQTGTMKNAARIPAGCVTLADALNSGGYETCYVGKWHIGG
                     AGNGPIPKELRGGFSDFIGYQCYNGFIHNVCFYDRDDREHCFDRHRTDVTTDLAIEKL
                     RGLAPHPFALFVSYQAPHYPVQPSPEFERMYAGVTIQRRPNSQEIDPYTRTWSPPSPW
                     PPDSCPDFQRYGNNLNEYLRLYYALVTQIDAGVGRILAELERLGRAEDTVVVFTSDHG
                     DMQGSHGLKNKSLPYEESSGIPLIVRVPGGADGGRTGALVSGIDFFPTCLEWLGLPQV
                     PGLPGRSFAPLSRGETQELNGPVFSEMPNWKMVCEGDLKLVVEGEQFTPKMLYDLAAD
                     PYEMENLVDRREYAAEVAALRERIVDWQAQSVTG"
BASE COUNT         1145 a         2057 c         1885 g         1228 t
ORIGIN      
        1 accgcgcctg ccggcttttc aggcctcaag gtgccgcatg gaccggtgaa gcgccaacgg
       61 cgcgcaacag cagagcccgg ctcgcagggc cgggcgggga tgaccggccc ataccaatcc
      121 cccacaccga taacgtcatt cccgcgaagg cgggaaccca gaaacagcaa gggcagcacc
      181 acgcctgccg gcccttcaga ctccgaagcg ccacctttcc gcttcaccct ccgtggccgg
      241 caaattgaat cgcgtacagg tcggcatctg acagcacgaa ccgcaaacgg accggctgaa
      301 ccgcgaaacg ggacacatca gggcccccct tccacgatac cgtccgcgcc agttcgtcgc
      361 cgatcacctc aacgcagtca tccagcgcga agccttccag cggcttgcca ttcgccccct
      421 gcatttccac cctgatgctt cccgccgccg acgtggacat gttcagcgta agccgcgagc
      481 cggaaaacgt gaacggcttc gtcaccatct caccaccggc aaacggcgcg ttcacagaca
      541 cgaacccatc cattcgcagc gtgtagcggc ggatgaggtt ggcattgccc cgccagtagc
      601 cctccacaac gtagagcgag aactccggcg gcgcgcccgc gatatcggac tccgtttcca
      661 gcagtcccca cgcctggtac atgtcgccgt atgtccagct cccttccgtg cgcagccccg
      721 ggcggaggaa ggcctcgttc cagcgatgga acgtctggcc gtcgcggctg gccatgaaca
      781 gaccgtccgt cacggcggcg ccgtaccgct ccgacgctcc cgcgcgtaac cggcggtgct
      841 ccagttcggg caaggcctcg attgccgggc tccatttgcg ctccgtgtag cgcgtgggaa
      901 agcccaggaa aatgtgcggg gcgcgcggat acggcatgat ctggttcgtg tagagctgtt
      961 gcaccggcgc gcccggatac tccagccact tcggctccgt ccagttgacg aaatccggtg
     1021 aggtcgccgt tctgatgccc cgcactccgt tgtcgaaatc gcgcacgtat gcccggtact
     1081 ccccccgcag ggcgtcgtaa aacaccagat tctgcgaatc gaacggcagg ccgctgagga
     1141 tcggcttatc cccgagaatc cgccagcgaa tgccgtccgg cgacgccatg gcccacaggt
     1201 cgcgcttttt ctcagcacgc cccacggcct tgtaacgctg gtccgcgggg catgaaggat
     1261 tcacgtcaat gaacggcgca aacccgtgcg tgccccggat ttcatcccct tctccggccc
     1321 agacgaggtt gttccgccgt gagccatcca cttcgaacag accgtactcg ggacggtgcc
     1381 agtggattcc gtccagactc tccgcacagg caatattgct cttggtgtgc gtgagcgaga
     1441 ccgagccatc agcgttccga tcgaactgcg cgtgccacgt ggagtaatac attcggcaca
     1501 gcgcgccgtc gcgcagaacc gtgacgtagt ggcacatatt gccttcccac ggcctgtcgg
     1561 tgacgagggc aggctcccgc ggcacgggct ggtggagcag ttggcgggcg ccgtgcagtt
     1621 cgtcaatgag ccagtcgtcc acgaaaagct caagccggtc gccaatttcg cgcagggcat
     1681 ccatcgggcg tccctttcct tattgccgtt cggggtcgaa ccgcaggcct ttgagcggct
     1741 ttccggcgag atccatgaaa cggagcgaga aaccccaacc accaagccgg ttgtggcatt
     1801 tcacgaacaa ccggaaacgc cctttcggca acctgacgtg caccttgaac ttgtcccgtg
     1861 tcacaccgcc gctgccttcg tagcggtata cctcagtgcc gttgatgcgc acgatgcagt
     1921 cgtcgtcgct tgagatgccc atgagcgcgt cgcgctcgcg gtcgttttcc acgtcgctca
     1981 ccgcgtacgc cgttttgtgg acgatcagcc cccggttgaa caggttgctg atttccattt
     2041 ccccgccgac ggtttcccac gtccgccacc ggatctcccc gaacggaccc tcgtacacgg
     2101 catcgaaatc aatcgtctcg tcgggcggga acactgcgtc cagtgtcttg ccctcgggca
     2161 cgtcaaacgg agccgccacc cgccatgccg gaacgtagcc gggaacgtgg ttcggcgttg
     2221 tccagaagcc ctgtggttgc gttctgcgcc gcgccatttc cttcgtctcc ggccggacga
     2281 aaagcccgct ggtctggcgg atttcccggt acgccttcgc gatcggcttc tcaagttccg
     2341 gggtgtcaat ccatccccac ttcgacgact ggaacgcaat cagcccgcag gctccctcgc
     2401 gaacgaagtc ctccatctgt tcgcgcacct gttccgaagt cggcaggccc tgccctgagt
     2461 tcatcgggcg gccgtcgaac gactggaaaa cgccgatgaa tggaacgccg ggaacccgtt
     2521 cccgcgcctt cgcgagaatc cattgcacct cttcggaagt gctttcccgc atgtacctgt
     2581 cgggctccac ggggtaccag tagatcatca tgatgtcgca gatgccgggt ccaaagttga
     2641 gaaccgagcc cgcgtcgccg taccccgcgc acacgggctg ccggatctca gggtcgtact
     2701 tccggacgat gcggtacatc gcgcgcagag cggaacgcgc gtcgccgggg ctgtcgtcga
     2761 ggacgtacca cccgagaagg ttgggagagt ccttcacccg cagcacctcc tgctcacagt
     2821 cctccggcca gaagagtatc gtaccctccc ggtgccgcgc gggccgggtt ccgttccagc
     2881 cgcgtgcgga gccgatcaac gtgccgttct ccacgcgttc gtagcggatc agttcgtcgt
     2941 ccagcgcgat gacgagcgag tccgaaaaat gatgcctcgg ggcgagaaaa agcgggattt
     3001 cagtctgttc cgccgaaatt tcgttgcgga ggctgacacc gtggtacacg aacttttcca
     3061 gcggatagag aaccctgatc ccgcgttgcc gacaggcctt cccctcgggg gctgcgaggt
     3121 caaggtgcgt ttgcccggcg ataacggtgt tcatccccat cgaggcggcc ttttcgatct
     3181 cttcaacggt cggcacggta tagctgccga gaatcggcac gtaaccgatc tgaaatgcct
     3241 tgtgcagcag catgtgggtc gcgtcgctca tcggtcggtg ctccttcgag tgtcggtcgg
     3301 ggcctcttcg taacgtggca tgcctttggc cgcttgacgt gaattatgtg ccgggcggcg
     3361 cgtgttctca tggtacggcc gcctgaggga gcggtcaatg cggacggacg cgctcacgcg
     3421 caaggcgggt gattcggatg cagaacgcgg actttccatt cgtgtaggca acccggtcgt
     3481 tcggatattc gcgggccagc cgcagtttca gctcctcata ttcctttgcc gtttccgggt
     3541 gcgcgatgag gtaatcacgg aagagaaggc gctcccacag ggcggaatcc gcctccacca
     3601 tgtggatatg gtgcgtgcgc gaaccggcgc ggtcgcgctt gatgaaccac gcgtagaagg
     3661 gcggtacgtt gtcgccgaac aaaggccgcc agaagtagtc gtacccctgc gcctcaagca
     3721 ccggcgcgat gcgggttttc gtctcctcca gtgagcgcac ctccaccagc atgtcgatga
     3781 tgggcttcgc cgcgaggccg ggcaccgcgg tgctgccgaa atgctcgatg cggcgtatga
     3841 gcccgtccgg aaaactcgcc agcaggtgcg ccttttcctc cgcgaacatg cgcggccatg
     3901 cggggtcata cggcacgatg gccacatcct cgcgcaccac ccgggcaatc ctttgttcaa
     3961 gactttccaa attcattact cctgagccct ggcccggagg gacccgctcc gtcgtgacct
     4021 tccggttgtg gttaatcccg tcggaacgag gacccgacag agcgggtccc tccaactgcc
     4081 tccgcatgga accttcctct tgggaaccgc cattcgaccg gttcacaccc gttctcgggc
     4141 ggttctgtga gaaaccgctc gctctaccac ggcaggacgt tgatctcgcc ttgaaacggc
     4201 cattcgttcg ggtttccgca gagacgcttg cggacagggt tttggacaac ataatcccat
     4261 ttgtcctcgt acctctccga tctcctcagt tgtgtatccc agaacgactt ttgccagatt
     4321 ggttgctcgc tccgccgtgg ccagcgtatc gaggcatatg tcttccaaca tttcacccac
     4381 acggacaact cggggtggtc gggtccggca ggcgaacaaa acaggtgaac gtgatcaggc
     4441 atgatcaggt atttgcccac gagccagcct cgggtcgctt cccacgcctc acacagtgtc
     4501 tggtgcgctt ccctggtggc caggatcatt ttccggtcct tcgcgcacac cgtcaggaag
     4561 acgataatcg gtccttccgc gagcctgact gttggcatat ggaccggtga tttcctgcga
     4621 ttcattggga tttctttcaa ggacgccaag cgcgacactg ctgcttcatg gtcgtaactg
     4681 cccctgacgc gtctggaggg acccgctccg tcgggtccca aacgacctgc cccttgggtc
     4741 aaccggtcac gctctgcgcc tgccagtcaa caattcgctc cctgagggcg gcaacttccg
     4801 ccgcgtactc acgtctgtcc acaaggtttt ccatctcata cgggtcggcg gcgaggtcgt
     4861 aaagcatctt cggcgtgaac tgctctccct ccaccaccag cttcaggtct ccctcgcaca
     4921 ccatcttcca gttcggcatc tcggagaaca ccgggccgtt gagttcctgc gtttcgccgc
     4981 gggagagcgg ggcgaatgac ctccccggca gaccgggcac ctgcgggagg cccagccatt
     5041 ccaggcacgt ggggaagaag tctatccccg aaacgagggc tcctgttcgc ccgccgtcgg
     5101 cgccacccgg cacccggaca atgagcggga tgccgctgga ttcctcatac ggcagcgact
     5161 tgttcttcag gccgtggctg ccctgcatgt cgccgtggtc cgacgtgaac acgaccacgg
     5221 tgtcctccgc cctccccagc cgctcgagtt cggccagaat ccgccccacg ccggcgtcta
     5281 tctgcgtgac gagagcatag tacaggcgga gatactcgtt caggttgttg ccgtagcgct
     5341 ggaaatcagg gcacgaatcg ggtggccacg ggctgggtgg gctccaggtg cgggtgtagg
     5401 ggtcgatttc ctggctgttt ggcctccgct ggatggtgac gcccgcatac atccgctcga
     5461 actccggcga cggctgcacc gggtagtgcg gtgcctgata cgagacgaac agcgcaaagg
     5521 ggtgcggcgc gagccccctc agcttctcga tggcgaggtc ggtggtgacg tcagtccggt
     5581 gccggtcgaa gcagtgttct cggtcgtccc tgtcgtagaa acagacgttg tggatgaatc
     5641 cgttataaca ctgatacccg atgaagtcag agaacccgcc ccgcagctct ttcggtatcg
     5701 ggccgttgcc cgcgccgccg atgtgccatt tgccgacgta acaggtttca tatccgccgc
     5761 tgttcagcgc atcggccagc gtcacacacc ccgcaggaat ccgcgccgcg ttcttcatcg
     5821 tcccggtctg ggaggaatag agaccggtaa aaagattgat acggaatggt gtgcataccg
     5881 ggcagttgga gtacgcccgc tcgaacagca tcccgcgctg tgccagtgcg tcgaggttcg
     5941 gggtgtgaat gtccggcgtg cccatgcagc gcagcgcata acggtgcatc tgatcggtga
     6001 agatgaagag caggttcacg atggctcctt tgcagaacga gaggactggt cagaaaaata
     6061 cggtggaccg gggtttcgtc catccccgcg cgaatcggca ccacgcgggg gatggtgcca
     6121 cggttgccgg agacgcgggc cacacctact ttgtaaatgc cgcatcattg tccggcacgt
     6181 aatgtgtgac gaagagacat ttcgcaggag accacggtgg atttgctcga ccacgggttc
     6241 tgggactaca caacgccgta tgccggcgga atggaagcgt acaggcggga tgactatctc
     6301 cggcttgtgg atgac
//
LOCUS       DAOSOO010000184         6300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12481_length_6300_cov_6.065172, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000184 DAOSOO010000000
VERSION     DAOSOO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12481_length_6300_cov_6.065172"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLT86_11460"
     CDS             <1..821
                     /locus_tag="PLT86_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37269.1"
                     /translation="ITSEQADRLITWAWQTPWQAPRKVAIVAEADFITELIAPKLLKT
                     LEEPPPAVTFLLITSRPQNLLPTVRSRCQVVPIPPLTEDEIVRILVEHGADEGDARSA
                     AMLANGDVWVAQSLTRAGAEGTRRNAYSLIETALDPKKKPVHIFDLCRKLLAPPEGKT
                     RWLPSEEAAIVGEITRWMAWGLRNILVNLEESSEVPEELRSIFPSAKRIGFARLFSWL
                     DEVDRIHEMSIRNVSAMPALFALAMYLRDDRRVFRETDGSEIAAFPSLTWWPSL"
     gene            840..1781
                     /gene="ricT"
                     /locus_tag="PLT86_11465"
     CDS             840..1781
                     /gene="ricT"
                     /locus_tag="PLT86_11465"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOT37270.1"
                     /translation="MKNHDIEPDEKPLEELEDRDLHLQAEEEAPVEESAELEGEIVEV
                     VFRGNRRAVYVNAHNVQLARGNHVIVEADKGEDLGRVCQTGGKTRAGRRAKKKTVLRL
                     AESHEISRWNALRTKEEEALRDFQQRISRRKMPMKPVDVEYQLDGKKICFYFTADHRV
                     DFRELVRELASVYRTRIELRQIGVRDEAKRLGGIGVCGRELCCATFLAEFAPITSQMA
                     RDQNLSLNPSKLSGICGRLKCCLRFEQEFYRESHERFPRLGTAFAANGHSGTVVKIDF
                     LHETVTLRTEDQDEWTVSLKGTTATHSSRHPKNSNAN"
     gene            1792..2619
                     /locus_tag="PLT86_11470"
     CDS             1792..2619
                     /locus_tag="PLT86_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT37271.1"
                     /translation="MKWTEMTAPALADLSPDHVLLLPLGSCEQHGQHLPVFTDTFITT
                     AIADSVEKRIPHRVVQLPTLWLGASHHHRLFAGVLSLSESVYMTVLTGILECLADGPV
                     CAPGHAKRILLLNGHGGNVYPGQAALSEFAWKFRSRPDLIAAFASYWTLSDDAIKTVP
                     METPALTHSCEYETSLMLAIKGELVDMSKAKAGNLHWQHTRFTPDASRPSKVGVAAPF
                     HARSSNGALGHPELSSEEKGKQLLDVIVHDTIAFIEEFVRWDDLKDVRAAQSGEVAE"
     gene            2616..3548
                     /locus_tag="PLT86_11475"
     CDS             2616..3548
                     /locus_tag="PLT86_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT37272.1"
                     /translation="MKVLLIGGTRFLGLAIANALHRHGHKAVVAHRGHTKAALPETAR
                     EIHVDVADQKSLENCLLAEHYDAVVDTILGAKTLEWIVTTLNGRIRQFVHCGSTGVYA
                     PMKRIPATEEDPCEALPQYGGFGAKLEQDEVLMRAHRQTGFPATVLRPTNIYGAGDIP
                     LDIWGARNPAFFRRLTRNQPVTLPNDGRALLQPGHVSELGEAFALALESPHSTGRIYN
                     ISSARAVELRVYLDILKGILKSSSPVDFASAELLLERYLPENKINEGGLRFVVEHMCV
                     DISRARAELGFDPQISLEEGLAENLQWMREKGLI"
     gene            complement(3641..4843)
                     /locus_tag="PLT86_11480"
     CDS             complement(3641..4843)
                     /locus_tag="PLT86_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOT37273.1"
                     /translation="MKPHAVSWDAKTFGARGDGARDDTASFQAGLDAVASGGGGTLSV
                     PPGRYRICGPLEVRANVTLQGAGGAVNGWGEPTVFEVFDREHPFLSLSNGGGVHNIDI
                     VYPEQRLRAGEVVPYPYTIVARGENCHVIGVSLRNPYNGICLDYAARHLVRDVHGSPL
                     NIGLFVNQCYDVGRVENVHFWPFMGPEENKEFYLEYLPHHATAFVFGRTDWEYVFNTF
                     CWGYKVGYHFAETEQGSTNGNFVGIGADATEMCLLVDQAQPCTGGILVTNGEFVPLMS
                     PDCSALVVGETFRGYISLVNCGVWGPHDSSARIAGDGRVNIASCNFIDWDKNLTDAPV
                     IDCRGGVLTLNGNHFTRSQNKKRSTPQVALRKGTRGAIVTGNFSDNPWRVINEIGDTA
                     QIGFNLGT"
     gene            complement(4862..5755)
                     /locus_tag="PLT86_11485"
     CDS             complement(4862..5755)
                     /locus_tag="PLT86_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT37274.1"
                     /translation="MTPAKDLWAFWEATKAELADTPADATMTPAPEMSTESVLVYHVS
                     MAGFGGRRIGGWFGTPPVPPGVRIAGLLWLPGYGGIPEAATALDFASKGFATLVLEPR
                     GQGKGNAFPNGSDGKLVHGITDPHGYAYRAVVMDCVRGLQILQNQPEVHPDRVAVAGG
                     SQGGGLTLMTASVCDESVRVVAAHVPFLCHFDFVIEHAIETAPYVNLTRYLAEFPERR
                     EIARRTLSYFDPVNLVSRLRAPVLLTLGGRDTTCPRETIEAVFEAVPGKKALLVEPDA
                     RHEWLDDTRTHMVSWLNHYLG"
     gene            complement(5752..>6300)
                     /locus_tag="PLT86_11490"
     CDS             complement(5752..>6300)
                     /locus_tag="PLT86_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT37275.1"
                     /translation="RNLAADVLGSLVRYTGFLTRIADNPFGISKFYTGDAEAFFYQFP
                     EESSWHVGQNSQYLSEAWAAFLIFQVTHRKEALSHGLKQLDWVLGANPYGICMLHGEG
                     RVNTATAHHRYNTIVGQPQGAVPGSVFNGITRFRPECDVPLWDLLEKGTPAYECNEPW
                     IPHNAYYLLAVTELAAVCEGTR"
BASE COUNT         1393 a         1890 c         1780 g         1237 t
ORIGIN      
        1 aaatcacttc cgaacaggcc gatcggctca ttacgtgggc atggcagact ccgtggcagg
       61 cgccgcggaa agtagcgatc gtcgcggaag ctgacttcat caccgagctg atagccccaa
      121 aacttctcaa aacgctggaa gagcctcctc ctgcggtgac tttcctactc ataacgtcca
      181 ggccgcagaa ccttcttcca accgtccgca gccggtgtca ggttgttccg atcccaccgc
      241 tcactgaaga tgagatcgta cggatcctcg ttgaacacgg cgccgatgaa ggcgatgccc
      301 ggtctgccgc catgctggcc aacggagatg tgtgggttgc gcaatcgctg acacgcgcgg
      361 gtgccgaggg gacgagacgc aacgcgtaca gcctgatcga aactgcgctt gaccctaaga
      421 agaagcctgt gcacatattt gacctttgcc gaaagcttct cgcccctccg gaagggaaaa
      481 cccggtggct tccttctgag gaagccgcaa tcgtggggga aattaccaga tggatggcgt
      541 ggggtctccg caatatcctt gtgaatctcg aggaatcatc ggaagtgccc gaagaactac
      601 gcagtatttt tccttcagcg aaacggattg gtttcgcccg gttgttttcc tggctggacg
      661 aagtcgacag aattcatgag atgtcaattc gtaacgtgag cgcgatgccc gcccttttcg
      721 cgctcgcaat gtatctgcgc gacgacaggc gggtcttccg ggaaacggac ggctcagaaa
      781 ttgccgcatt tccctctctg acctggtggc cttctctttg atcggcaacg gaatctcgaa
      841 tgaaaaacca cgatatagag cccgatgaaa aacccctcga agaactggag gatcgcgatc
      901 tccacctcca ggccgaggaa gaagcgcccg tagaggagtc cgcagaactt gaaggagaga
      961 tagttgaggt ggtttttcgc ggaaaccggc gggccgtgta cgtcaacgca cacaacgtgc
     1021 agcttgcgcg cggaaaccac gttatcgtcg aggcggataa gggagaggat ctggggagag
     1081 tgtgccagac aggaggcaaa acgcgcgcag gacgacgggc aaagaagaaa accgtcctcc
     1141 gattggcgga atctcacgaa atctcccgct ggaatgctct gcgaaccaaa gaggaagagg
     1201 cgttgcggga ttttcagcag aggatttcgc gccgcaaaat gccaatgaaa cccgtggatg
     1261 tggagtatca actggacggg aagaaaatct gcttctattt caccgccgac caccgcgtcg
     1321 atttcagaga actggtccgc gaacttgcat ccgtgtaccg gacacgcatc gaattgcgac
     1381 agattggcgt ccgcgatgaa gcgaagcgcc tgggtggcat cggcgtgtgc ggtcgcgagc
     1441 tgtgttgcgc aacattcctc gcggagttcg cccctatcac gagccagatg gcgcgagatc
     1501 agaacctgtc gctcaatcct tccaaacttt cgggcatctg cggacgcctc aaatgctgcc
     1561 tgcgttttga acaggagttc taccgggagt cccacgaacg gttcccaaga ctcggaacag
     1621 cgtttgccgc caacggccac tcaggaacgg tcgtgaaaat cgatttcctg cacgaaaccg
     1681 tcacgttgcg aaccgaggat caggacgaat ggacggtttc cctcaaagga acaacggcca
     1741 cccactcctc acgccatcca aagaactcaa acgcaaattg aggtaacacg aatgaaatgg
     1801 accgaaatga cggctcccgc tctggcagac ctgagcccgg atcatgtcct gttgttgccg
     1861 ctcggatcct gtgaacagca cggtcagcac ctgcccgtct tcaccgatac gttcatcacg
     1921 acggcaatcg ccgatagcgt ggagaaacgg attccacatc gcgtcgtgca gctccccacc
     1981 ctttggttgg gcgcttcaca tcatcaccgc ctcttcgcgg gcgtcctcag tctttccgag
     2041 agcgtgtaca tgaccgtgct taccggcata ctggaatgcc ttgccgacgg gcctgtgtgc
     2101 gctccggggc atgcaaaacg gatactcctt cttaacgggc acggtggcaa tgtgtacccc
     2161 gggcaggcag cactcagtga attcgcatgg aaattccgtt cccggcccga ccttatcgcg
     2221 gcattcgcaa gttactggac cctttccgac gacgccatca agacggtccc catggaaacg
     2281 cccgccctga cacactcatg cgagtacgaa acctcgttga tgcttgcgat taagggcgaa
     2341 cttgtcgata tgtcaaaggc gaaggccggg aacctgcact ggcagcacac gcgtttcact
     2401 ccggacgcga gccgaccatc aaaggtcggc gtagccgcac cgtttcacgc acggagctcc
     2461 aacggcgcgt tgggccaccc ggagctttcg tcggaagaaa agggaaaaca actgctggac
     2521 gttattgtcc acgacacaat tgcgttcatc gaagaattcg tgcgctggga cgatctcaaa
     2581 gacgtccgcg cggcacaatc aggggaggtt gcggaatgaa agtgctcctc atcggtggaa
     2641 ctcgtttcct cggccttgcg attgcgaacg ccttgcaccg ccacgggcac aaggcagtgg
     2701 tagcccaccg cggacatacg aaagccgcat tgcccgagac tgcacgggaa atccacgttg
     2761 acgtggcgga ccagaaatcg ctcgagaact gcctcctggc cgagcactac gatgcggttg
     2821 tggatacgat actcggcgca aaaacgttgg aatggatagt cacgaccctc aacggaagga
     2881 ttcgccagtt tgtgcactgc gggtctaccg gagtgtacgc gcccatgaag cgcatacctg
     2941 caacggaaga agacccttgc gaggcgcttc cgcagtacgg cgggttcggc gccaaactgg
     3001 aacaggacga agtcctgatg cgcgcccacc gacagacggg gttccccgca accgtcctcc
     3061 gccctaccaa catatacggt gcgggcgaca ttccgctgga tatctggggc gcacggaatc
     3121 cggcattttt ccgacgcctc acgcggaacc agcccgtcac gttgccgaat gacggtcggg
     3181 cacttctcca accgggtcac gtctcggaac tcggcgaggc atttgccctc gcactggaat
     3241 cgcctcactc gaccggacgc atttacaaca tatcgagcgc ccgcgcggtt gaactccgcg
     3301 tctatctcga cattctgaaa gggatcctca agagttcctc acctgtcgat ttcgcatccg
     3361 ccgaattgct tctggaacgg tacctgccgg agaacaaaat caacgaaggc ggtctgcggt
     3421 tcgtggtgga acacatgtgc gtggacatct ctcgtgcgcg ggcggagctg ggattcgatc
     3481 cgcagatttc gctggaggaa gggcttgcag agaaccttca gtggatgcgc gaaaagggac
     3541 tgatctaact acggttgaac acgtttcctg cgtgcgcaga actccgcgca caccttgggc
     3601 aggcaaggtg caacggggct cgagcacccc ttttccggct ctacgtcccg agattgaagc
     3661 cgatctgcgc ggtgtcgcct atctcgttga tgacacgcca ggggttgtcg ctgaaattac
     3721 cggtaacgat tgcgcctcgt gtgcccttcc gcaacgccac ttgaggagtt gaccgtttct
     3781 tgttctgaga ccgggtaaaa tgattgccat tcagcgtcaa aacgcctccc cggcaatcga
     3841 taacaggtgc gtccgtgagg ttcttgtccc agtcgatgaa attgcacgac gcgatgttga
     3901 cccttccgtc tcctgcgatc cgcgcgctgc tgtcatgcgg tccccacacg ccacaattga
     3961 cgagactgat gtaaccacga aatgtctcgc cgaccaccag cgccgagcaa tcgggtgaca
     4021 tcagcggcac gaattcgcca ttcgtgacga ggatgccccc ggtacagggt tgcgcctgat
     4081 ccaccagcag gcacatttca gttgcgtcag caccgatacc cacaaagttg ccgttcgtcg
     4141 aaccctgttc cgtctccgcg aaatgatagc caaccttgta tccccagcag aacgtgttga
     4201 acacgtactc ccagtctgtt cgaccaaaga cgaatgccgt cgcgtgatgc ggaagatatt
     4261 cgagatagaa ctccttgttc tcctcaggac ccatgaaagg ccagaaatgt acgttttcca
     4321 cccttccgac atcgtaacac tgattgacga acaggccgat gttcagcggg cttccatgga
     4381 cgtccctcac aaggtgccgc gcagcgtagt cgagacatat gccgttgtac gggttacgga
     4441 gtgatactcc gataacatgg cagttctcgc ctcgtgcaac gatcgtgtag gggtatggca
     4501 caacctcacc cgcgcgaagt cgttgctccg gatacacgat atcgatgttg tgcacgccgc
     4561 cgccgttcga gaggctcaga aacggatgtt cacgatcgaa cacttcgaag acggtcggtt
     4621 caccccaccc gtttaccgcg ccgcccgcgc cctgcagggt cacgttagct cgcacctcga
     4681 gcgggccgca gattcggtac cttccggggg gaacggacag cgtcccgcct ccgcctgaag
     4741 caacggcgtc cagaccggcc tgaaacgatg ccgtatcatc ccgcgcaccg tctcctctgg
     4801 ctccgaacgt cttggcgtcc cacgagaccg cgtgtggctt catgtccgac ctcatactgc
     4861 ttcagccaag gtaatgattc aaccacgaga ccatgtgcgt tctcgtgtca tccagccatt
     4921 cgtgcctcgc atcgggttcg acaagaagcg ctttctttcc cggcacggct tcgaacaccg
     4981 cctcgatcgt ctcccgcgga cacgtggtat cgcggccgcc aagcgtcaga agaaccggtg
     5041 cacggagtcg cgacacgaga ttgaccgggt cgaagtaact caacgtcctc ctcgcgattt
     5101 cgcgccgttc cgggaactcc gcgagatagc gtgtcaagtt tacgtacgga gcggtttcta
     5161 tcgcgtgctc aatgacgaaa tcaaagtggc aaaggaaggg gacatgcgcc gccaccacgc
     5221 ggacgctctc gtcgcataca gaggcggtca tcagagtcag gccaccgccc tgactacctc
     5281 ccgcgactgc aacacgatcg gggtggactt cgggctggtt ttgaaggatc tgcagaccgc
     5341 gcacgcaatc catcactacg gcccggtacg cgtaaccgtg gggatccgtt atgccatgca
     5401 caagcttccc gtccgaccca ttagggaacg cgttgccttt gccctgaccg cgaggttcaa
     5461 gaacaagcgt ggcaaaccct ttggacgcga agtcgagcgc ggttgccgct tcggggatac
     5521 ctccgtaccc gggcagccac aacagacctg cgatcctgac accgggagga acagggggag
     5581 taccgaacca gccgccgatg cgacgcccgc cgaaacctgc catgctgaca tgatacacca
     5641 acaccgactc ggtcgacatc tccggtgcag gagtcatcgt cgcgtcagcg ggggtgtcgg
     5701 ccagttcggc ttttgtcgct tcccagaatg cccagagatc ctttgcagga gtcatctagt
     5761 gccctcgcag acggcggcca gctctgtcac ggccagaagg tagtacgcgt tgtggggtat
     5821 ccacggctcg ttgcactcat acgccggtgt tcccttttcg agcaaatccc agaggggcac
     5881 gtcacattct ggacgaaatc gcgtgatccc gttgaacaca gaacctggaa ccgcgccctg
     5941 cggttgtcca acaatcgtgt tgtaacgatg gtgtgcggta gccgtgttca cccggccctc
     6001 accgtggagc atacagatgc cataggggtt tgcgccgaga acccagtcga gttgcttgag
     6061 accgtgagaa agcgcttctt tccggtgagt aacctgaaag atcaaaaagg cagcccacgc
     6121 ctcggagaga tactggctgt tctgaccgac atgccagctc gattcctccg ggaactggta
     6181 gaagaacgct tccgcgtccc cggtgtagaa tttcgaaatg ccaaatggat tgtccgcgat
     6241 acgggtaagg aagcccgtgt atcggaccaa gctcccgaga acgtcggcgg cgaggttcct
//
LOCUS       DAOSOO010000185         6223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12680_length_6223_cov_4.934176, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000185 DAOSOO010000000
VERSION     DAOSOO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12680_length_6223_cov_4.934176"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PLT86_11495"
     CDS             complement(<1..1274)
                     /locus_tag="PLT86_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOT37276.1"
                     /translation="MPGFEGRGGGHHYGMGPVSGPVLPGGDIGNGVAGLPESVRAAFL
                     KSAENGEEILVAATSDLSPDGRYGQQHVLASKNRLCLAVPNGDQPNLSMWPLKELKKA
                     NVDLLVGQASLVATIGKDRVELCRFTNSKQREFHRLAALLEELARNGAMPVEKADPKD
                     SLPRYCSRCSRLLPEPGGTCPACLKRGQVLIRLLGYLRPHWKRAALLIATMVVSALIE
                     TLPPYLTKVLIDDVINRPHVTTIAGWSADHLGWSRVAWLALIVTLFLLSRILLVGVGI
                     LGGRQATWLGPRIVADLRAKLYNHLQRLSLNYFDKSRTGALISRVTHDTAHVQGFLVN
                     GVPHVGIDLFLLVFIGMILFSMNWQLTLAILVPLPIVIYVSKNFWRFMRSLFGRAWAR
                     SARLVATLNDSLNGIRVVKAFGQEEQEIARFNS"
     gene            1457..2524
                     /locus_tag="PLT86_11500"
     CDS             1457..2524
                     /locus_tag="PLT86_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37277.1"
                     /translation="MAKMTPRQRVEAALRGEWADQVPFTIYANKLPRCQVELELRNAG
                     VCVLERRSVVRAETPNVRVTSIGYEKGGVQFTRRVFSTPRGELSTIERPGIGTTWRVK
                     RMFTRPEDYAPLLALIKDQRYVPDFETFQKMREDLGEGAYLRAGIGYSPLQEIIYTYM
                     GVECFSIEWAERRDEVMRLYDALTEDRRKIYPLLAESPAEIINYGGNVSVEVVGKDRF
                     ERFIIPHYNEVAEIVHKRGKMLGSHLDGNNRQLAPLVANSLLDYVEAFTPPPDCDMTV
                     AEARELWKGKVLWINFPSSVHLSGVEAVENMMHRLLREAAPGDRFLVGVTEDVHEDAW
                     RKTFPAMARILNESGRLPLER"
     gene            complement(2585..3070)
                     /locus_tag="PLT86_11505"
     CDS             complement(2585..3070)
                     /locus_tag="PLT86_11505"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOT37278.1"
                     /translation="MDPKPVSDSSVTMTELVLPSHANPLGTVFGGVIMSWIDICAAIA
                     AQRHARRVVVTASIDMLHFRAPITVGCVVHLKAQVNHAWHSSMEVGVRVDGENPLTGE
                     TRHAATAYLTYVALDEKRRPVEVPAVLCETPEQERRSRDAQARREKRLANRSIGEYES
                     R"
     gene            3297..4271
                     /gene="pfkA"
                     /locus_tag="PLT86_11510"
     CDS             3297..4271
                     /gene="pfkA"
                     /locus_tag="PLT86_11510"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT37279.1"
                     /translation="MQRIGVMTSGGDAPGMNANVRAVVRTGIFRGVQVFGIRDGFAGL
                     ISDEIIPMTSPSVGGIVGRGGTILGTARCEEFRDPAGRAKAAGTLRKHGIEGIVVCGG
                     DGSFRGAHLLWKEHNIPVICTPGTIDNDLAGTDYTIGFDTAVNTALEAIDRIRDTAEA
                     HRRTFIVEVMGRHSGFIALATAIGGGADAVLVPEIPPDIPRIVSMVERRFAAGKRFSL
                     IVVAEGVVIDDFPGAGSLAHILKKHGLDCRVSILGHQQRGGAPTALDREFASLLGKEA
                     VEELLRGGTDRMAGIVGKRVEFCDLEFSWREKKPIDMEVYRFISEAAI"
     gene            4468..5565
                     /locus_tag="PLT86_11515"
     CDS             4468..5565
                     /locus_tag="PLT86_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT37280.1"
                     /translation="MARRITIADIARYAGVSPATVSLVLNGKYTQGQTARGTAERVWE
                     AARVLGYRGRIRMRPEPGAPAIGLLIADRESFFTTYYGPRLLKGMWHVADEYNAHLFL
                     FSDRMEGQPVTFDYLRKELESRRVNDLVVNVNNNLSESLLDTVEALAIGGTRIVALWR
                     RPKSWFAPCVAPDDENAALLMLSHLFELGHRRVALVATEPDERTLLITGRFAAAAREM
                     GVDLAFGEALFENGGKWGVLDDDQAVARLLGCSSPPTAIATLYDGRAVGVTKALGSLG
                     YRVPDDISLIAYGNMPENRPTPYLITMVQSPIEQIGETAVRLLVDPESSYSACCAGPP
                     IRLPVALRVGATTGPASKAGPYSRSRVLRSF"
     gene            5631..>6223
                     /locus_tag="PLT86_11520"
     CDS             5631..>6223
                     /locus_tag="PLT86_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37281.1"
                     /translation="MNSLRAVWMAIAAVFLANAADSRESATIRITVGPDRSDWPHAPV
                     AIRLDSILSRNGWKSPLGNSLIRVRESGNARLVPSRAGWNHSGECPGLLLFSKTGPGR
                     REYVITVTSDEATPAGERIPVVGFGEPLTHGVTGIVSGIDGSFNSNLAVADWDGDGDL
                     DFFVAQGQGGGANWTMHGLLYYENVGTSRSPLMAKPR"
BASE COUNT         1302 a         1700 c         1932 g         1289 t
ORIGIN      
        1 gagttgaacc gggcgatctc ctgctcttcc tggccaaagg ccttcaccac gcgaataccg
       61 ttgagcgagt cattcagcgt tgccacgagg cgcgcacttc gcgcccatgc tcttccgaag
      121 agcgaacgca tgaaccgcca gaagttcttg ctgacataga tgacgatagg aagaggcacc
      181 agaatggcca gtgtgagctg ccaattcatc gagaaaagta tcattccgat gaacacaagc
      241 agaaacaggt cgatgcccac gtgaggcacg ccgtttacca ggaatccctg aacatgggcg
      301 gtgtcgtgtg ttacacggct tatgagagcg ccggtgcggc tcttgtcgaa atagttcaga
      361 ctgagtcgtt ggaggtggtt gtagagcttc gccctgagat cagcgacgat gcgagggcca
      421 agccacgtcg cctgccgccc gccaaggatg cccacgccga cgagaagaat gcgcgagagc
      481 aggaaaaggg tgacgatgag tgcgagccac gcgacccgtg accaccccag atggtcggca
      541 ctccacccgg cgatagtcgt cacgtgggga cgattgatta catcgtcaat cagaacttta
      601 gtcaggtacg gcggcaaggt ttctatcagc gccgagacga ccattgtcgc gatgagcaat
      661 gcagcccgtt tccagtgggg acgcaggtat cccaacaagc gaatgagtac ctgacctctc
      721 ttcaggcacg cggggcacgt gccgccgggt tccggcaaaa ggcgggagca ccttgagcaa
      781 taccgtggta acgagtcttt cgggtcagcc ttttctaccg gcatcgcgcc attgcgcgcg
      841 agctcttcaa ggagcgccgc cagccggtgg aattcccgtt gtttggaatt cgtgaaacga
      901 cagagctcca ccctgtcctt gccaatcgtg gccaccagcg aagcctgtcc cacaagaagg
      961 tctacgttgg cttttttcaa ctccttgagc ggccacattg aaagatttgg ctggtcaccg
     1021 ttcggtacag ccaggcacag acggttcttg cttgccagaa cgtgttgctg gccgtatcgg
     1081 ccatccggtg acaggtcact tgtggccgca acgaggatct cttccccgtt ctcggcagat
     1141 ttgagaaacg cagcgcgcac ggattctggc agacccgcca cgccgttgcc gatatcaccc
     1201 ccgggaagaa caggtccgga aacgggcccc atgccgtagt gatgtccccc gcctcgccct
     1261 tcgaaacctg gcatgccaac ctctcggttc ggagaaaccc ctgcgaacga gtcttcgcat
     1321 tgtttcgcgt tatacagata agatatcgct ttgaacggag tgtcccaatg ttccagacat
     1381 cagtgattgc gttgctggcg tgggagccgt attctcgtca ctgcaatgtt ctgtcacgcc
     1441 agcggagggt tggcacatgg cgaaaatgac cccgcggcag agagtggaag ccgcgttgcg
     1501 aggagaatgg gcggaccagg ttcctttcac catttatgcg aacaaactcc ctcgctgcca
     1561 ggtggaactg gaactgcgga acgccggggt gtgcgtgctg gaacgacgga gtgtggtgcg
     1621 cgccgagaca cccaacgtca gagtgacctc tatcgggtac gagaaaggag gagtgcagtt
     1681 cacgcgacgg gttttcagca cgccacgcgg agaactgtcc accatcgaac ggcccggtat
     1741 tggcactacg tggcgcgtca aacgcatgtt cacgagaccg gaggactatg ccccgcttct
     1801 cgcgctgatc aaagaccagc ggtacgtgcc cgatttcgaa actttccaga aaatgcggga
     1861 agaccttggc gagggcgcat atctgcgagc gggaatcggt tactctcccc tccaggaaat
     1921 catttacacc tacatgggtg tggagtgctt cagcatcgag tgggcggaac ggcgggacga
     1981 agtcatgcgt ctttatgacg cgttgaccga agacagaagg aaaatctacc cgttactggc
     2041 ggaaagtccg gcagaaataa tcaattacgg tggcaacgtg agcgtcgagg ttgtggggaa
     2101 ggatcgcttt gagcgcttca tcattccgca ttacaacgaa gtggcggaaa tcgttcacaa
     2161 acggggcaaa atgctcgggt cgcatcttga cggaaacaac cggcagcttg cgccgttggt
     2221 ggcgaactca ttactcgatt acgttgaggc atttacgcct cctcccgact gtgacatgac
     2281 ggttgctgaa gcccgagagc tgtggaaagg caaagtactg tggatcaact tcccatcctc
     2341 ggtccacctc tccggagtgg aagcggtcga aaacatgatg caccgccttc tcagggaagc
     2401 agctccgggt gatcgtttcc tcgtaggtgt gaccgaagac gtgcacgaag acgcgtggcg
     2461 aaaaacgttt cctgcaatgg caaggattct caacgaatcg ggaaggttgc cactggaacg
     2521 gtgaaacaca tcccggagtt cggtcaggcc aaggcgcctt caagcctgtc cgcgggggca
     2581 ccagtcaacg gctttcgtac tcgccgatcg atcggttcgc gagcctcttt tctcgccgcg
     2641 cctgggcgtc cctcgaccgt cgttcctgct cgggtgtttc acacagaaca gcgggaacct
     2701 ccacagggcg ccgcttctca tcgagggcca cgtacgtcag gtaggcagtg gcggcatggc
     2761 gcgtctcgcc ggtaagcggg ttctccccat cgacccgcac tcccacttcc atggacgaat
     2821 gccaggcgtg attgacctga gccttgaggt gtaccacgca gccgacggtg atcggggcac
     2881 gaaagtggag catgtcgatg gaagcagtga ccacgactct tctcgcgtgg cgttgcgccg
     2941 caatggcggc acagatatcg atccatgaca taatgacgcc cccgaaaacg gttcccaggg
     3001 gatttgcgtg gctcggtagc acgagttccg tcatggtgac tgaggaatca gaaacgggtt
     3061 tgggatccat gcaatggttc cgtggaaaca ggtttgccgg cgcgtgacgt atttcagtat
     3121 cgttgaaatt gcgtgtttgt ggaacagatg ccaaggcacc aacggtagca cggccgccgc
     3181 gggctcttct gttttgctga tttttgtccg gcgctggcgt ttgctccggt gttcacggca
     3241 accggagtga accggctacg caccggcacc gaggttgaat aatcgcggag aaacagatgc
     3301 agaggatcgg tgtgatgacg tcgggaggtg acgctcccgg gatgaacgca aacgtacgcg
     3361 cagtcgtcag aactggaata ttcagaggcg ttcaggtatt cggaatccgg gacggattcg
     3421 ccgggcttat ttccgacgag atcatcccga tgacgtcgcc gtccgtcggt ggcatagtcg
     3481 gcagaggtgg tacgatactt ggaactgccc gttgtgagga gtttcgcgat cccgccggcc
     3541 gcgcgaaagc cgcgggaaca ttgcgcaagc atggcatcga agggatcgtg gtctgtggcg
     3601 gagacgggag tttccgcggt gctcatctcc tgtggaaaga gcacaacatt cccgttatat
     3661 gtacgccggg aacaatagac aacgatctcg cgggaactga ctacaccatc gggttcgaca
     3721 ctgcagtgaa caccgcgctg gaggcgatag acaggatacg cgatactgcc gaggctcacc
     3781 ggcggacctt catagttgaa gtgatgggta gacactccgg gttcatagcg ctggccacag
     3841 cgatcggtgg aggtgcggat gcggtgcttg ttcccgagat ccctccggac atcccgcgga
     3901 ttgtctcgat ggttgaaaga cgttttgccg ccggcaagcg tttttcgttg atcgtcgtcg
     3961 cggagggagt cgtaatcgat gatttcccgg gagcgggaag tctggcgcac atcctgaaaa
     4021 agcacgggct tgactgccgc gtttccatcc ttggtcatca gcagcgaggc ggcgcaccta
     4081 ctgcgttgga tcgcgaattc gcgtctctgc tcggaaaaga agctgtggag gaacttctgc
     4141 gcgggggaac ggacagaatg gcgggaatag tcgggaaacg cgtggaattc tgtgacttgg
     4201 agttctcgtg gcgcgaaaag aaaccgattg acatggaggt gtaccggttt atcagcgaag
     4261 ccgcgatttg acgtcggccg ttgaggttac tctcgcagcc ttcctcaaga ctgcccgtgc
     4321 cgcgaatcga gacccttcgc gcagtttgcg atgggtgtgc cggttcccgt ataatacttc
     4381 acgacgttgg atacagggtt ccttgcgttc cgtttcttct gcggaccgtt ttcgcggaaa
     4441 caagccgggg taaaaagaga tcgggaaatg gcgcggcgaa taacgattgc ggacattgcg
     4501 cgttatgcgg gcgtatcccc cgcgacggtt tccctggttc tgaacgggaa atacacgcag
     4561 gggcagactg caaggggaac cgccgaacga gtgtgggaag ccgccagagt cctgggttac
     4621 cgaggacgca taaggatgcg cccggaaccc ggcgcgcccg ccataggcct gctgattgcc
     4681 gatagagagt cctttttcac aacctattat ggcccgcggc ttctcaaggg aatgtggcac
     4741 gtcgcagatg agtacaacgc ccatctgttt ctgttcagcg atcggatgga agggcagcct
     4801 gtaaccttcg attacctgag aaaggaactg gaatcccgcc gcgtgaatga ccttgtcgtg
     4861 aatgtgaaca acaacctgag cgagtcgttg cttgacacgg tggaggcgct cgcgataggg
     4921 ggcacgcgga tcgtggctct gtggcggagg ccgaagtcgt ggtttgcgcc gtgcgtggct
     4981 cctgacgacg agaacgccgc gctactcatg ctgtctcacc ttttcgagct cggacaccgc
     5041 cgtgtggcgc tcgttgcgac tgaaccggac gagcgcacgt tgctcatcac cggaaggttc
     5101 gccgccgcgg cgcgggagat gggtgtggac ctggctttcg gcgaagctct ttttgaaaac
     5161 ggcggcaagt ggggcgttct cgacgacgac caggcagttg cccgattgct gggctgttct
     5221 tctcctccga cggccattgc taccctgtac gatggtcgcg cggttggggt aacaaaggcg
     5281 ttggggtctc tcggttatcg tgttccggat gacatctctc tgatcgcgta cggaaatatg
     5341 ccggaaaacc gacctactcc ttacctgatt acgatggtcc aatccccgat cgagcagatc
     5401 ggggagaccg cagtacgcct tttggtggat ccggaatcct cgtattcggc atgttgcgcc
     5461 gggcctccaa tcagactccc agtcgcactc cgggtgggcg cgaccacggg gccggcaagc
     5521 aaggccggtc cgtactcccg ttcacgcgtt cttcgctctt tctgacccgg gcaggttcct
     5581 tccgggtttc ccagtttttc tgccacgtat ttcgttcgga gaacaagacc gtgaattcgc
     5641 tcagagcagt atggatggcg atcgccgccg tgtttctcgc aaatgcggca gattcacgag
     5701 aatcagcaac aatacgcatc acggttggcc cggatcggtc ggactggcct catgcgccgg
     5761 tggcgattcg cctggatagc atcctgtcaa gaaatggatg gaagtcgccg ctgggcaact
     5821 cgttgattcg agtgcgagag tcagggaacg ctcgtttggt cccgagcagg gctgggtgga
     5881 accacagcgg tgagtgtccc gggcttcttc tcttctcgaa aacgggtccg gggcgacggg
     5941 aatacgtgat tacggtgaca tccgacgaag cgactcccgc gggggaaagg attcccgttg
     6001 tcggttttgg tgagcctctg acgcacggcg tgacagggat tgtgtcgggc atagacggca
     6061 gtttcaacag caatctcgct gttgcagact gggacggaga cggagatctg gatttcttcg
     6121 tagcgcaagg ccagggcggg ggcgccaact ggaccatgca cggccttctg tattacgaga
     6181 atgtgggtac ctctcgctca ccgctcatgg cgaaacctcg aag
//
LOCUS       DAOSOO010000186         6219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12693_length_6219_cov_6.122485, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000186 DAOSOO010000000
VERSION     DAOSOO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12693_length_6219_cov_6.122485"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..1260)
                     /locus_tag="PLT86_11525"
     CDS             complement(529..1260)
                     /locus_tag="PLT86_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37282.1"
                     /translation="MPFDLHRQLQELYAGEDGQREVRINGSFVADVVRHGVIYEIQTR
                     RLSNIKSKIASLLETTPIVIVYPVSVHTTIVHCDGADLRECDRRPSPKQGQALDAFSE
                     TVYLAHLLAHPNMALEILLVDTEDIRIKGDPHAATRRRRRRRRHSEWTTVNRRLTATH
                     GRVRLDTPADGLQLLPPAVKFPFCTRDLANATGASLSRAQQILYTLFHMGALRRVGRT
                     RSGWSYEPAQTEQGFPSGHASGPTP"
     gene            complement(1276..1677)
                     /locus_tag="PLT86_11530"
     CDS             complement(1276..1677)
                     /locus_tag="PLT86_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT37283.1"
                     /translation="MGRTGWLLMVVAMWGTANFLMKVVGSRISSASGVLGIVIGYSAI
                     GLWIALSGGGRIGTGVGYLGALAVGALYIGGNYAYLRLAQREDISAIAPIGSLNVVIP
                     VALGFLLLGEPMTLRKLVGFACALAAVVLLR"
     gene            complement(1682..2179)
                     /locus_tag="PLT86_11535"
     CDS             complement(1682..2179)
                     /locus_tag="PLT86_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37284.1"
                     /translation="MPQKIMSEIWDYIAQEFRDTISRATPEQLGWRITPKTYSFAETA
                     WRALGAAYLWSVVLSGAKTLEEAITMDPAALDLLQAALNKYAPGHFPDSMPCDQDTLL
                     ERTDEVIALLSAQLVEMSPSMRTKDFRTWWDAVYTGDEIVARLMWRVAHFNGHMHSIL
                     LHARG"
     gene            complement(2238..>3225)
                     /locus_tag="PLT86_11540"
     CDS             complement(2238..>3225)
                     /locus_tag="PLT86_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOT37285.1"
                     /translation="TRNSPVSSPKRVLILQPIHDAGLTALRNAGLDIVHAPDATRETL
                     LRLIPEADGLIVRTTAYRIDAEVMDASPRLKVIGRHGVGVDHIDLAAAEARGITVVNT
                     PLANSQGVAEYTIGLMLSACRFIPRADRAVREGNWPERERLVGDELFRHTLGIIGLGR
                     VGTRVARIAALGFQMRVLYFDIIRKHETEAALGVEFAPFEEVLRQADFLTLHTPLTEA
                     TRGMMNEATLALMKQGAYLVNASRGEVVKIDALARSIATGKIAGAALDVFPDEPFPAD
                     HPLLAMPEVIVSPHMASHSRDSMVRMALVAEDVARVLNGQEPLHPVGTGTVS"
     assembly_gap    3226..3325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3326..3711)
                     /locus_tag="PLT86_11545"
     CDS             complement(<3326..3711)
                     /locus_tag="PLT86_11545"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT37286.1"
                     /translation="MKDQKNIREILAKAHEKLDSANVLFEHQQYDDAISRAYYAVFHA
                     MTAALFFQDMVFSSHAQTIGAFNREFVKSGVFPVAFTTTIQSLFQNRQVADYGAGEAA
                     DRDTALSSLNGATAVVTAIEKYLADA"
     gene            complement(3708..4028)
                     /locus_tag="PLT86_11550"
     CDS             complement(3708..4028)
                     /locus_tag="PLT86_11550"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT37287.1"
                     /translation="MEAADARERAAYFSAALRKRLGTNLRQVILFGSRARGDFREGSD
                     YDFAVVVRRKDPTVQSSIVEAGVDFLNRFDEVSACVVYDETEWAESTRLPLGVNIKRE
                     GIAL"
     gene            complement(4127..5680)
                     /locus_tag="PLT86_11555"
     CDS             complement(4127..5680)
                     /locus_tag="PLT86_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37288.1"
                     /translation="MPAALRVACPTQVGGMVPRWGGPPHSKDRNGDHPMLNYLTGQPL
                     EIGPGPHLFLDDHLIEDRRQLTRRVNPPYRHPGNPIIVADRPWEERPYRPQVIRNPET
                     GGWRMYYMCFSGTNYWSRKGPSYYACYAESADGIRWEKPERADLPFDPWPATNVLHVD
                     DERDARIQAPWVFFDPHDTDPAHAWKMIYNSAGLRLAYSPDGIHWTPARPEPLFAYHS
                     DTMNHVLWNERAGKWVLYMRPPMFSSGRHEGPGVEHHRRRTAVSFSDDLLHWSVPRTV
                     LYPDELDPTPHIDSVHVFPYAGHYIAFVTMLDATTGGTNELMLASSRDGLEWERPLPR
                     QVWLSRGREGDFDGGCVSVGSDPIRVGWELWFPMSGFPMPQKVFEQESGIGIVKMMVD
                     RFLSLEAPEPSLGGPEDFGWLLTKEFIWRGKTLTINCRMNNGDSRTYGDLRVEVVKRP
                     NDADPAGRMGQVVPGHGVEDCDLIRSNSPVQTVTWNGNPDLGFLDGQPVYLRFRIRNG
                     GLFAFRMGE"
BASE COUNT         1143 a         1960 c         1846 g         1170 t
ORIGIN      
        1 actttcagtc aggcggcatg tattattgct tcaacacgca gaaggtggag gtcactatcg
       61 ggtaagcgcg gaaagcccct tttttcctcg ccatttgtgc ccacactggt aatggacgcc
      121 catcacacac tcccgccttg ccggcgccat accggtagca ttgtccctgt ggttgaccac
      181 ctcggtttcg acgagctgag gacagtggca cgaacatgaa acacggcgcc acaataaaca
      241 ctacctgaca agtcaggatt tctggtgcat gtgcttagta ataagtgtgg tttacaacgc
      301 attagataca agaaccgact tgatttaaag cgaataagaa taatattttg gaaaccagtc
      361 ccaatcctag gaaaactggt ggataagtgg tgaataactt tgtcgctgtg gtgaaaaagt
      421 ggggtcaaat gtggggtggt tgacccctct ccacctgaac tctccacctt gggggataac
      481 tggtggacag cgctcgtcag gctctgtaat acccggcagt gcagtgattt acggcgtggg
      541 tccagaagcg tgccctgatg gaaagccttg ctcagtctgc gccggttcgt aactccaccc
      601 ggaccgtgtc cgcccgacgc ggcgcagggc gcccatgtga aaaagtgtgt aaaggatctg
      661 ctgggcgcgg gagagcgagg cccctgttgc gttcgcgaga tcacgggtgc agaaagggaa
      721 tttcacggca ggaggcagaa gctgaagccc gtccgcggga gtatccagtc ggacgcgtcc
      781 atgtgtggcg gtaagacggc ggtttaccgt ggtccattct gaatgtctgc gtcgtcgccg
      841 tcttcttctg gttgccgcgt gagggtcccc cttgatgcgg atatcctctg tgtcaaccag
      901 aagtatctca agcgccatgt tggggtgcgc aagcaggtgt gcgagataca cggtctcgct
      961 gaaggcatcc agcgcctgac cctgtttcgg ggacgggcgc cggtcacatt cgcgaaggtc
     1021 cgccccatcg caatggacga tggtggtgtg aacagaaacg gggtagacaa tgacgatcgg
     1081 cgttgtttcg aggagggacg cgatcttgct cttgatgttg ctcagcctgc gcgtctgtat
     1141 ttcgtagatg actccgtgcc gcaccacgtc ggcgacaaaa gagccattga ttcgtacctc
     1201 gcgctgacca tcctcgccgg cgtaaagctc ctgcaactgg cggtggaggt cgaaaggcat
     1261 gccgcaaccc gcaccctacc gcaacagcac aacagcggca agggcgcacg cgaacccgac
     1321 cagcttccgc agtgtcatag gctcgccgag aaggagaaac ccgagcgcga ccggaatcac
     1381 cacattcaag ctgccgattg gcgcgattgc gctgatgtct tcccgctgtg ccagccgcag
     1441 atacgcgtag ttgccgccaa tgtaaagggc accgacggcg agtgcgccga ggtaaccgac
     1501 tcctgtgccg atcctgccgc cgccggagag ggcaatccag aggccgatag cggagtaccc
     1561 gatcacgatt cccagcaccc cgctggcgct tgaaatacgg gagccgacca ctttcatgag
     1621 gaagttcgcc gtcccccaca tggcgacgac catgagcagc cagccggtac gtcccacggt
     1681 gttatcccct tgcgtggagc aggatgctgt gcatgtgccc gttgaaatgc gccacccgcc
     1741 acatcaaacg cgcaactatc tcgtcccccg tatacacagc gtcccaccac gtcctgaaat
     1801 ccttcgtgcg catcgaggga ctcatctcca ctaattgagc gctgaggagg gcgatcacct
     1861 catctgtccg ctcaaggaga gtgtcctgat cgcagggcat gctgtccggg aaatggccgg
     1921 gagcgtactt gttcagcgcg gcctggagaa gatccagcgc ggccgggtcc atggtgatcg
     1981 cctcttccag tgtcttcgcg ccgctcagca cgacagacca gaggtacgct gctccgagcg
     2041 cgcgccacgc agtctccgcg aacgagtacg tcttcggcgt gatgcgccag ccaagttgct
     2101 ccggtgtcgc gcgggaaatg gtatcacgga attcctgagc gatgtagtcc cagatctcgc
     2161 tcatgatttt ctgcggcatg agttggttct cctgtgcggt tggcatgcgc cggggaagaa
     2221 agcgcagaga aagatactta cgaaacggtc ccggttccca caggatggag cggctcctgt
     2281 ccgttgagaa cgcgcgcgac atcctcggcc acgagagcca tgcgcaccat gctgtccctg
     2341 gagtgcgaag ccatgtgcgg gctgacgatc acttccggca tcgccagcag ggggtggtcg
     2401 gccggaaacg gctcatccgg gaacacgtcc agcgccgccc cggcgatttt ccctgtggcg
     2461 atgctgcggg caagcgcgtc aatcttcacc acttctcccc gggatgcatt gacgagatac
     2521 gcgccctgct tcataagcgc cagcgtggcc tcgttcatca tcccgcgggt tgcctccgta
     2581 agaggcgtgt gcagcgtgag aaaatccgcc tggcgcagga cctcctcaaa gggcgcgaat
     2641 tccaccccga gcgccgcctc ggtctcgtgc ttgcggatga tgtcgaagta gagcacgcgc
     2701 atctggaacc ccagcgcggc aatccgggca acacgcgtgc cgacccgccc cagcccgatt
     2761 attccgagag tgtgccggaa caattcatcg cccaccagcc gttctcgctc cggccagttc
     2821 ccttcgcgga ccgcccggtc ggccctcggg atgaaacggc aggcggaaag catcagcccg
     2881 atcgtgtact cggccacgcc ctgcgagttg gccagcggcg tgttcaccac ggtgattccc
     2941 cgggcttccg cggccgcgag atcaatgtgg tccaccccta ccccgtgccg gccgatcacc
     3001 ttcagccgcg gggaggcatc catgacctcc gcgtcaatgc ggtatgccgt cgtgcggaca
     3061 atgagcccgt ccgcctccgg gatcagcctg agcagcgttt cgcgagttgc gtcgggcgca
     3121 tggacgatgt ccagcccggc gttcctgagc gctgtcaggc cggcatcgtg aatcggttgg
     3181 aggatgagaa cgcgtttggg ggatgacacc gggctattcc tcgttnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnttcgc gtccgcgagg tacttctcaa ttgctgttac
     3361 taccgccgtt gcaccattga gactcgaaag cgctgtgtcg cgatcggcgg cttctcccgc
     3421 gccgtagtcg gccacctgcc tgttctgaaa aagggactgg atggtggtgg taaacgcgac
     3481 cgggaacaca ccacttttca cgaattcgcg gttgaatgcg ccaattgtct gggcgtggga
     3541 cgaaaatacc atatcctgga aaaagagggc cgcagtcatc gcgtgaaaga ccgcataata
     3601 cgcccgggaa atggcatcgt catactgctg gtgctcaaaa agcacgttcg cggagtcaag
     3661 tttctcgtgc gcctttgcca gaatttcccg gatgtttttc tgatccttca cagcgcaatt
     3721 ccctcacgct tgatattcac tccgaggggc aatcgcgtgc tctcggccca ctcggtctcg
     3781 tcgtacacca cacaggcgga aacctcatcg aaacgattca ggaaatccac acctgcctca
     3841 acaatcgagg actggacggt gggatccttc cggcgtacca ccactgcgaa atcgtagtca
     3901 gaaccctcgc ggaagtctcc ccgggcacgc gaaccgaaca ggatcacctg ccgcagattc
     3961 gtgccgagcc ttttccgcag ggctgcggaa aagtaggcag ctcgttccct tgcatctgcc
     4021 gcttccatgg ttgacccccg gcacctggcg ctccgcacct tccctcttga aaataacctc
     4081 acgatacgct tttcgcccgg tagtggtcag cggctcatct tccgcttcat tcgcccatcc
     4141 gaaacgcgaa cagacccccg ttgcggatgc ggaagcggag atacaccggc tggccatcca
     4201 gaaatccaag gtccggattg ccgttccacg tcactgtctg caccggcgaa ttcgagcgga
     4261 tgaggtcgca gtcctccacc ccgtgccccg gcacaacctg ccccatgcgc ccggcggggt
     4321 cggcgtcgtt cggccgcttc accacctcca cccgcaggtc gccgtacgtc cgggagtcgc
     4381 cattgttcat ccggcagttg atggtgagcg tcttcccgcg ccagatgaac tccttcgtca
     4441 gcagccaccc gaaatcctcc ggcccgccga gggagggctc cggcgcctcg agcgacagga
     4501 accggtccac catcatcttc acgatgccga ttccgctttc ctgctcgaac accttctgcg
     4561 gcatggggaa cccggacatc gggaaccaga gctcccaccc gacgcggatc gggtccgagc
     4621 ctaccgacac gcacccgccg tcgaaatcgc cctcgcgtcc gcgcgacagc cagacttgcc
     4681 gtggcagtgg cctttcccat tcgaggccgt cgcggctgct tgccagcatc agctcgttcg
     4741 tgccgccggt ggtggcgtcc agcatggtga cgaacgcgat gtagtgcccc gcatagggga
     4801 acacgtgcac cgaatctata tgcggcgtcg ggtcgagttc atccgggtac agcaccgtgc
     4861 gcggcaccga ccagtgcagc aggtcgtcgc tgaaggacac cgccgtgcgc cggcggtggt
     4921 gctccacgcc ggggccttca tgccggcccg aggagaacat cggcggccgc atgtagagca
     4981 cccatttgcc cgcccgctcg ttccacagca catggttcat cgtgtcgctg tggtaggcaa
     5041 acagcggctc cggacgcgcc ggagtccagt gaatcccgtc cggcgaatac gccagccgca
     5101 gccccgcgga gttgtagatc atcttccacg cgtgggccgg gtcggtatcg tgcggatcga
     5161 aaaacaccca cggcgcctga atccgcgcgt cccgctcgtc gtccacgtgc agaacgttcg
     5221 tcgcgggcca ggggtcgaat ggcaggtcag cccgctccgg cttttcccag cggataccgt
     5281 ccgcgctttc ggcgtaacac gcgtaatacg aaggcccttt gcgcgaccag tagttcgtcc
     5341 cgctgaaaca catgtagtac atccgccagc cgcccgtctc cgggttgcgg atgacctgcg
     5401 ggcggtacgg gcgctcctcc cacggccggt ccgccacgat gatcgggttg cccgggtggc
     5461 ggtagggcgg gttcacgcgg cgggtaagct ggcgccggtc ctcgatgagg tggtcatcca
     5521 gaaacaggtg cgggcccggc ccgatttcaa gcggctgtcc ggtcaggtag ttcagcatgg
     5581 ggtggtctcc gtttctgtct ttggagtgcg gcggcccgcc ccagcgggga accatgccgc
     5641 caacctgggt gggacaggcc acacgcagtg cggcaggcat tcctgcctgt ccttaaccca
     5701 tcggtgtccg cgccagcccg gtcctgcgaa ccgggctgag ctatctcgcg ccgttggcgc
     5761 ttttttgaca gcacacgtcc accgaaaggc ctgaagggcc gcaatattca tagcccggct
     5821 catcgggccg ggcggtgtgg ccggtgcgtc ggcctggatt cccgccttcg cgggaatgat
     5881 ggcgtgtttc aggctcagcc ggcgcgcgtc caccccgtcc actgtgtcca ccgggcaggc
     5941 aaaaatgcct gccgcccgcg gggcggcacg ccccagcggg gtaccatgcc gccaacctgg
     6001 gtgggacagg ccacacgcag tgcggcaggc attcctgcct gtccttaacc ctcggaatcc
     6061 ccgccagtgc ccggtcctgc gaaccgggct gagctatctc gcgccgttgg cgcttttttg
     6121 acggtacacg tccaccggaa ggcctgaagg gccgcaatat tcatagcccg gctcatcggg
     6181 ccgggcggcg tctccggtgc gtcggcctgg attcccgcc
//
LOCUS       DAOSOO010000187         6210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12719_length_6210_cov_5.602437, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000187 DAOSOO010000000
VERSION     DAOSOO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12719_length_6210_cov_5.602437"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLT86_11560"
     CDS             complement(<1..281)
                     /locus_tag="PLT86_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37289.1"
                     /translation="MFSLNARMARPRIGLLPTGHYYYWQQFPELRAKGLAMYGRLLER
                     LGTFGDVIAPELVDTPEKSAAAGELFLKERVDIVLVFPFGYTPAMCVVPA"
     gene            complement(520..1467)
                     /locus_tag="PLT86_11565"
     CDS             complement(520..1467)
                     /locus_tag="PLT86_11565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT37290.1"
                     /translation="MSPRTVSIVIPLLDEEESLRPLHEAITRVVDREGYVAEIVFVDD
                     GSRDGSFSVIRELAEKDPRVKAVRFRRNFGKSAGLATAFRVATGDIVVTMDADLQDDP
                     EEIPNLIAKLDEGYDLVTGWKVIRHDPISKTLPSKVFNGVTSFITGIKLHDFNCGLKA
                     YRRELTKSIRMKRGMHRFVPALAHWQGFRVAEIPVQHHPRRFGKSKFGAKRFVDGFLD
                     LLTVVLLTRYVSRPLHFFGWVGTFLMVGGVAIEGYIAYLRLVHGWIMNRYPLLWLGVL
                     MIIVGLQMLSTGLLAELVANVKGDDGEVSILETIRAEGV"
     gene            complement(1473..2465)
                     /locus_tag="PLT86_11570"
     CDS             complement(1473..2465)
                     /locus_tag="PLT86_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT37291.1"
                     /translation="MSWWRSPWVSRVLKLLVSVVLIGGIVWQVGPDAVLPTSFSWWYG
                     IAAVAVFVLSNLLGAYQWNLLLKIAGIELPKRVVTRAYFISLFFNNFLIGNVGGDVLR
                     AFEVRKNASPGLTHTTASGVATIVMDRFLGFFTMMIFAGAAALFTEEHSEFGALIAAV
                     LGSFVVLGVLITSRRIGTRVDACVVGLLPERVSRTITGLRAGFVAMRARWVVLGMAAA
                     VSFLVQGLRIYVHYLCALSIGVDIPLIYFWTFIPLIGVAAAIPISSGGLGPREWAAKW
                     LFTTLPGVGAGGVVAMELLAHVTALASSLPGAVAFVLRRESTRPPEPSQDLSTG"
     gene            complement(2462..3484)
                     /locus_tag="PLT86_11575"
     CDS             complement(2462..3484)
                     /locus_tag="PLT86_11575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT37292.1"
                     /translation="MSVVIPHYAGSQLFECLEALFSDTDAPGEVLVVDDASTDGTSEV
                     AQRRYPGIRLLRNKTNLGFVGACNRGLAEAAGAYAVLLNDDALPQPGWLQPLRERMER
                     DPQIAACQPKILRAHNSAIFDYAGAAGGLLDRYGYPFALGRWFDVCEADAGQYDAPRE
                     IMWASGAAMMLRMSVYRDIGGLEPVFQMHMEEIDWCWRARVAGYRIVSVPESRILHYG
                     AATLKAESLRKMYLNHRNSLLLLVKNYEARTLVRVLPVRLLLELVTIFGGLVSLNWRR
                     ALGAAWGLVGAVAALPRILPARAQVQRLRRVRDHEIARYLYQGSIVLRWLLRQGAPAE
                     IPGAGA"
     gene            complement(3584..>6210)
                     /locus_tag="PLT86_11580"
     CDS             complement(3584..>6210)
                     /locus_tag="PLT86_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37293.1"
                     /translation="AMHAMNARRLLGDEWAEPASLYGLSEDPMAYLQISIVLAVIFGV
                     AGVVVTFLTKTVKTRDIWAAIFVAGAAAATVQSLTGVFFWVENVQPFPTSRVVGALGL
                     LVILYFLSQSRERPSIPILKGYHLLVGMLLLVVVGYSTYLFLMIRSGLNPTIDENNPE
                     NWKNLFDFLARKQYGNEDMSMIIFSRKASMQYQFWDMFIKYLLHQFPMSISGALFDWK
                     LTFRSAMEVSYFGMRVPDFPLLLMFLGLGWHFNADRKRFFGMLALFIISGLGLVIYLN
                     MPDPQPRERHYVFTGAISVMAIWMGMGVTGLIRTAASWLPASVPEWLRTKFLPVLMAL
                     IGFAVPVWFLLGYPLVDEYSGDFSVRYSNFVKHDRRYDTVGYDYAYNILQSCDRDAVL
                     FTNGDNDTFPLWYVQEVAGVRKDVRVVNLSLLNTDWYIQQLRDNEPKLPMSASWTNDW
                     ITNTLCGNTLESLISSGRIDLTPNGTPYDENGRVVGWVTKPVTAGRVDSAYIELKNGE
                     IRRGILGVPITGSTVLKEYGKAVWDTIPDAEVRVRLIGQSFPGITWDMPASPEYRVLR
                     VQDVMIFKILDWVKWQRPVYFAVTVASDNLIGLDKYLRMEGMVLRITNQADSTYVEGR
                     GSYALDIERSKHNLDNVYQIRHMMDQRIYKDDNMLKLVSNYRSAYLRLAEKQIDTGDT
                     LGARETLVKFNQRIPRDWRGAYYGATVARRGGAQLDSLTQGYLREASQALLNELPTMH
                     VFDIYSLERVRMTAQLLRSTGQEQEAIRLLLATDPYLQKPSPLSGISSADRIGLFFEA
                     STIYDQVGDIQNAREMLERCRNLLMVIPNTAEANQEFARTFRTDAMTLAGEVNRRIAD
                     LEQRARQTTPRSDSLPRR"
BASE COUNT         1326 a         1960 c         1795 g         1129 t
ORIGIN      
        1 gcaggaacaa cgcacatggc cggggtgtag ccgaatggaa acaccagaac gatgtccaca
       61 cgctccttga ggaagagctc gcccgcggcg gcggattttt ccggcgtgtc caccagctcc
      121 ggcgcgataa catccccgaa cgttccgaga cgctccagca gcctgccgta catggcgagg
      181 cctttggccc tcagctcggg aaattgctgc cagtagtagt agtgcccggt cggcagcagg
      241 ccgattcgcg gccgcgccat gcgcgcattc aggctgaaca tctgtcaact ccgtgtgact
      301 gcagggttgt cggacttgcg aatggctctc gtcatcggct tgggggcgcg cgggagggat
      361 atcacccggc aaaggcgaac acgcgaccca acgaaaacgg aaggcgttgt ctaccctcgc
      421 ggggagactg cccggtgaca caacggtgag ccttttggga agcgcgtggg agggcatgtc
      481 tacgcaacga gagagggtcg cgcatatcgg gcggtcgggc tatactccct ccgcccgaat
      541 ggtttcgaga atggagacct ctccgtcatc ccccttcacg tttgccacca gttccgccag
      601 cagacctgtg gagagcatct gcaggccgac gataatcatc agcacgccga gccacaggag
      661 aggatagcgg ttcatgatcc atccgtgcac cagccgcagg tacgcgatgt atccctcaat
      721 cgccacgcca cctaccatga gaaacgttcc cacccagccg aagaagtgaa gaggacggct
      781 gacgtaccgc gtcagcaaca ccacggtgag caggtcgaga aagccgtcca cgaagcgttt
      841 cgcgccgaat ttgctctttc cgaacctgcg ggggtggtgc tgaaccggta tctctgccac
      901 gcgaaacccc tgccagtgcg cgagagccgg aacgaaccgg tgcattcccc gcttcatgcg
      961 aatgcttttc gtcaattcgc ggcggtaggc cttcaggccg cagttgaagt cgtgcagctt
     1021 gataccggtg atgaaagacg tcaccccgtt gaaaaccttg gacggcaggg tttttgaaat
     1081 cgggtcgtgg cggatcacct tccagccggt aacgagatcg tatccctcgt ccagcttcgc
     1141 gatgagattc ggaatctcct ccggatcgtc ctggagatca gcgtccatcg taaccacgat
     1201 atcgcccgtg gcaacccgaa acgccgttgc cagcccggct gattttccga agttgcgccg
     1261 gaaccgcacc gccttgacac gtggatcctt ctccgcaagt tcacggataa ccgagaaaga
     1321 accatcacgg ctcccgtcgt caacaaacac gatctccgcg acatatccct cgcgatccac
     1381 caccctggtg attgcttcgt gaagcggacg gaggctttct tcctcgtcca gcaaggggat
     1441 cacgatcgag actgtccgtg gagacatgag tgtcatcctg tggagaggtc ctgtgaaggc
     1501 tcaggcgggc gcgtggattc cctccggagg acaaacgcca cggcgccggg aaggcttgag
     1561 gcaagtgccg tcacgtgtgc cagaagctcc atggctacca ctccccccgc gccgaccccg
     1621 ggcagcgtcg tgaaaagcca tttggctgcc cattcccggg gcccgagtcc acccgaactt
     1681 atcggaatcg ccgcggcaac cccgataagc gggatgaacg tccagaagta aattaacggg
     1741 atatccaccc cgatggacaa cgcgcacagg taatgcacgt aaatccgcaa cccctgaaca
     1801 agaaacgaaa cggctgccgc cattcccagc acgacccagc gggcccgcat cgccacaaag
     1861 ccggctcgaa gcccggtaat cgttcgcgac acgcgttcgg gcagcaggcc caccacgcac
     1921 gcatctacgc gcgtcccaat gcgccggctc gtgatcagga cgcccagcac cacgaaactg
     1981 ccgagaaccg ccgcgatcag agcgccaaac tccgaatgct cttccgtgaa aagagctgcg
     2041 gcgcccgcaa agatcatcat ggtgaagaac ccgaggaagc gatccatcac gatggtggcg
     2101 acacccgaag cagtggtgtg ggtcagtccg ggagaagcgt ttttcctgac ctcgaacgcg
     2161 cgcagtacgt caccacccac gtttccgatg aggaagttgt tgaaaaacag ggagatgaaa
     2221 taggcgcgcg tgacaacgcg ttttggcagt tcaattcccg cgattttcag aagcaggttc
     2281 cactggtagg ccccaagcag gttgctgagg acaaacaccg cgaccgccgc gataccgtac
     2341 caccatgaga aggaagtcgg caaaacggca tccggtccga cctgccagac aatccccccg
     2401 atcagcacga cgctgaccag aagcttgagc acgcggctga cccacggaga gcgccaccag
     2461 ctcatgcgcc ggctcccgga atctccgcgg gagcaccctg gcgaaggagc cagcgaagga
     2521 cgatactgcc ctgatacaga tagcgagcga tttcgtggtc gcggacgcgc ctgagcctct
     2581 gcacctgtgc ccgtgcgggc agaatccggg gtaatgccgc caccgcgccg accagccccc
     2641 acgccgcgcc cagtgcacgc cgccagttca gtgagacgag tcccccgaaa atggtcacca
     2701 gctcaagcag aaggcggaca ggcagcacgc gcacaagcgt tcgcgcctcg tagttcttca
     2761 cgagcaatag gaggctgttc cggtggttaa gatacatctt gcgaagggat tccgctttca
     2821 acgttgcggc gccgtaatga aggatcctcg attccggcac gctgacaatg cgatacccgg
     2881 ccacccttgc gcgccagcac cagtcgattt cttccatgtg catctggaac accggctcca
     2941 gcccgccgat atcacggtac acgctcatgc gcagcatcat cgccgcgccg gatgcccaca
     3001 tgatctcccg cggggcgtcg tactgaccgg cgtccgcttc gcacacgtca aaccagcggc
     3061 cgagcgcgaa ggggtacccg tagcggtcga ggagaccacc cgccgcgcca gcatagtcga
     3121 aaatcgcgga gttgtgcgcg cggaggattt ttggctgaca cgcggcgatc tgcgggtcac
     3181 gttccatgcg ttcgcgcagc ggttgcaacc agcccggttg cgggagagcg tcgtcattca
     3241 acaaaacggc gtatgcgccc gccgcttccg caagcccgcg attgcacgcg cccacaaaac
     3301 cgaggtttgt cttgtttcga agtagccgaa tgccgggata gcgccgctgt gccacctccg
     3361 aggtgccgtc ggtggacgcg tcatccacca ccagcacctc tcccggtgcg tccgtatctg
     3421 aaaacagcgc ctcaaggcat tcaaaaagct gcgatcccgc atagtgcgga atcacgacgg
     3481 acacgtcatc ccgtgcgatc gtgctccctt tcttcgaaag gccgcctttt tgtgaagggc
     3541 agcctttccc ttgaaaccgg aatgaccctt ttcccgcgct ctttcaccga cgcggcagcg
     3601 aatcgctgcg cggtgtggtc tggcgtgctc tctgttccag atccgcgatg cggcggttca
     3661 cttcgccggc cagcgtcatg gcgtccgttc ggaacgtccg cgcaaattcc tggttcgctt
     3721 cggcggtatt gggaatcacc atcagcaggt tccggcagcg ctcgagcatt tcccgcgcat
     3781 tctgaatgtc cccgacctgg tcataaatcg tcgaagcctc gaaaaacagg ccgatacggt
     3841 ccgcgcttga gatgccggaa agcggggacg gtttctgcag atagggatcc gtggccagca
     3901 gtagcctgat cgcctcctgc tcctggcccg tggaacggag caactgcgcc gtcatgcgaa
     3961 cacgttccag cgagtagatg tcgaacacgt gcatggtggg cagttcgttc agcagcgcct
     4021 gggaagcctc ccgcagatac ccctgtgtca ggctgtcgag ctgtgcgccg ccccgacggg
     4081 caaccgtagc gccgtagtac gcaccgcgcc aatcccgcgg gatgcgctga ttgaacttca
     4141 cgagcgtctc cctggcgccc agcgtgtcac ccgtatcgat ctgcttctcc gcaagccgca
     4201 ggtaggcgga gcggtagttc gagaccagct tcagcatgtt gtcgtccttg taaatccgct
     4261 ggtccatcat gtgccggatc tggtatacgt tgtccagatt gtgctttgag cgctcgatgt
     4321 cgagtgcgta cgagccgcgc ccttccacgt aggtgctgtc cgcctggttg gtgattcgaa
     4381 gaaccatgcc ttccatccgc aggtacttgt cgaggccgat caggttatcg gacgccacag
     4441 tgactgcgaa gtagacgggc cgttgccact tcacccagtc gaggatcttg aaaatcatca
     4501 cgtcctgcac acgaagcacg cggtactcag gcgaagccgg catatcccac gtgattccgg
     4561 ggaaggactg accaatcaac cgaacacgca cctccgcatc cggaatcgta tcccacacgg
     4621 ccttgccgta ctccttcagc accgtgctgc cggtaatcgg aaccccgagg atgccgcgcc
     4681 ggatctcgcc gtttttcagc tcgatgtacg cagaatccac ccttcccgcc gtcacgggtt
     4741 ttgtcaccca gcccaccacg cggccattct cgtcgtacgg cgtgccattc ggagtgagat
     4801 caatccggcc cgaactgata agcgattcaa gcgtgttgcc gcacagggtg ttggtgatcc
     4861 agtcgttcgt ccagctcgcg ctcatgggaa gtttcggctc gttatcccgt agctgctgga
     4921 tgtaccagtc ggtgttcaaa aggctcaggt tgaccacccg cacatccttc cgcacgccgg
     4981 ccacttcctg aacgtaccag agcgggaagg tgtcattatc cccgttggtg aacagaacgg
     5041 cgtccctatc acacgactgg aggatgttgt acgcgtagtc gtaaccaacc gtgtcgtacc
     5101 tgcggtcgtg tttgacgaag ttggagtagc gcacgctgaa atcacccgag tattcgtcca
     5161 cgagcgggta tcccagcagg aaccacaccg gcacagcaaa cccgataagg gccatcagga
     5221 cggggaggaa tttcgtccga agccattcag gcacggacgc aggcagccac gacgccgccg
     5281 tccgaatgag gccggtcacc cccatgccca tccagatcgc catcaccgaa atcgcgccgg
     5341 tgaacacgta atgccgctca cgcggttgcg gatcgggcat gttcagatag atcaccagcc
     5401 ccagaccgga gatgatgaac agggcgagca tgccgaaaaa gcgctttcta tcagcgttga
     5461 agtgccaccc gaggccaagg aacatgagca ggagcggaaa atcgggcaca cgcatcccga
     5521 agtacgaaac ctccattgcc gacctgaacg tgagcttcca gtcgaacaga gctccggaga
     5581 tgctcatggg gaactggtgc agcaggtatt tgatgaacat gtcccagaac tggtactgca
     5641 tgctggcttt gcggctgaag atgatcatgg acatgtcttc attgccgtac tgcttgcggg
     5701 caaggaagtc aaagaggttt ttccagttct ccgggttgtt ttcgtcaatc gtggggttca
     5761 atccggaccg gatcatgagg aagaggtacg tggaatatcc gaccaccacc agaagcaaca
     5821 ttccgaccag aaggtgatac cccttcagga tgggaatgga gggtcgctcc cggctttgcg
     5881 agaggaagta caggatcacc agcaacccga gagcgccgac cacgcgcgag gtcgggaagg
     5941 gttgcacatt ttcaacccag aagaacactc ccgtaagcga ctgaaccgtt gccgccgcgg
     6001 ctccggcaac gaatatcgcc gcccagatgt cgcgagtttt caccgtcttc gtgaggaacg
     6061 tcaccaccac gcctgccacg ccgaaaatca ctgccagcac gatgctgatc tgcaggtagg
     6121 ccatggggtc ttcgctgagg ccgtacagcg acgccggctc cgcccattcg tcgccgagca
     6181 gacggcgcgc attcatcgca tgcatcgcga
//
LOCUS       DAOSOO010000188         6175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12806_length_6175_cov_4.904739, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000188 DAOSOO010000000
VERSION     DAOSOO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_6175_cov_4.904739"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PLT86_11585"
     CDS             complement(<1..1603)
                     /locus_tag="PLT86_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37294.1"
                     /translation="MNTALVLAMSFFCASRAFPQDSPVVDSTAFHQGRVITEVDVVPG
                     DVVPPWWEEAVHTRRRLSRWTYATLNALHIKTKPHVVERELLFAVGDTLRQKSLDESE
                     RNLRAYSFINEATIEAYPADSNGVRVLVRTSDNFSLAAGWIWESGGGSDRIGALVTEK
                     NLLGFGKEIAAQYERERNRSRGTTETEWLFQYKDPRLIGSRFALQVSASRAYLGNEFM
                     TALARPFTTTRTRNAGGVEFYHFTGRMRLWRDKVPVAELPVTTTDTYAWVAHAWGEAS
                     KRTKLQAALRLRNTRHTDTPLLFATWLPRDTIRVSRVAYEPSFTISRQWTSAFHKLRN
                     LDDYDTVEDVESGTNIGLTTGFGLPSQPTHNPYGLAGAFAEWCAVRGEHVTAARLQVS
                     TRLADDQGSGRSAWSNREVAAYLHHYYTGLPWQTFALNVNWRSGERMDGPYQLVLGGD
                     RGLRGYSANAFEGNRRIVLNAEDRIFSPVRVLVYRLGFVVFADAGYVWQPDRRVDPRD
                     IRADIGAGFRVYNNRAATGRVSRLDI"
     gene            complement(1603..2712)
                     /locus_tag="PLT86_11590"
     CDS             complement(1603..2712)
                     /locus_tag="PLT86_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37295.1"
                     /translation="MNFWISALEPSGDRWGAALVRAIAGLAPNARVGGISGPLMRDAG
                     ADGTPAPDGVMGFAEPFVRFHEYCSLMARSVRQFRAHPPDVSIVIDAPDFHIPFLRFV
                     RQSRVPTVYFSPPQVWAWRRSRVRTIAHLCARVVTLFDWERAYFAPPMETDRVIWEGH
                     PLVDELPEPQEQRREGDLLILLPGSRKSEIDRLSVLFADVGRRLGVPCVFRAPTEHHA
                     GWIRTALSRAGRAPLPVTTSPLAQLLNEARACIACAGTATLEAALAGIPTVIAYRTDW
                     LTYSVARCLVHTRWIGLPNILLCSNAYPECVQEDAEASRIESALATVTERPPDFWVEK
                     AREIRTKLGTPGVANRVVKLAIETANDVPHPGIQQ"
     gene            complement(2709..3251)
                     /locus_tag="PLT86_11595"
     CDS             complement(2709..3251)
                     /locus_tag="PLT86_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT37296.1"
                     /translation="MARLAALGRFLFRHRDVLPVPLAVTVALSSRIRGGRLVSGALLV
                     LTGLAIRLWAVLYIGEQSRSRGDGPAFRTIGGPYAYFRHPLYLANGVLSEGLVLFSGA
                     GKRWLPFVFPVLAFLFYAPIVAWEQGRVYRRGIPVITHRFGVRTALCSERRTYQSVLA
                     FLLVSVVSSFVRNNLRRNRQ"
     gene            complement(3253..4038)
                     /gene="lpxA"
                     /locus_tag="PLT86_11600"
     CDS             complement(3253..4038)
                     /gene="lpxA"
                     /locus_tag="PLT86_11600"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOT37297.1"
                     /translation="MNHVDTRAVVEENAQLGSGVTVAPFAIVKAGAIANDGCTIGSYA
                     YVASGVRLGARVQVFSHACLGTIPQTKPQPPHEGFLIVGDDTVIREFTTLNSGSVLQD
                     RGGDGTTRIGARCLLMAYVHVGHDCTVGNDVILVNGATLGGHVVVEDFATISALVPVH
                     QFVRIGAYSYVAGGFRAVQDVPPYILAAGEPLRPYGLNVVGLRRHGFTAEQFRRLRAI
                     YRLFFRSGLNTAQALSRSSELEPSVERDRFVAFVERSQRGVIR"
     gene            complement(4035..4514)
                     /gene="fabZ"
                     /locus_tag="PLT86_11605"
     CDS             complement(4035..4514)
                     /gene="fabZ"
                     /locus_tag="PLT86_11605"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOT37298.1"
                     /translation="MSATADEPTVLDIRDIMDRIPHRYPFLLVDRIVSIREDGITGLK
                     NVTINEPYFAGHWPSEPVMPGVLQLEALAQTGAVLLFHRLAVDRQKTKLDVFFRGIDG
                     AKFRRIVRPGDQLLLDITVLKQRRELFVLKGEARVNGEVASEAELTAVLRVRGEEQP"
     gene            complement(4511..5410)
                     /locus_tag="PLT86_11610"
     CDS             complement(4511..5410)
                     /locus_tag="PLT86_11610"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOT37299.1"
                     /translation="MRHTVGRCVTVRGRGIHSGTDVTVRLLPADRPGITFVRSDLPGN
                     PRISAATSNLASSSRCTRLEKGDGAVMTPEHLLAACWGTGITDLVVELDSEELPIGDG
                     SAEIWCEAFFLAGTQETPGASVALGPLPPIHVAHAGACITLTPSKTWRVEYSGRFFDG
                     PEQHVEWTEETDFRQHVARARTFCTLSDALKLRKLGMIKGGTPENALLWVDDVSLLSD
                     PALPPWVEDQPRPEKGLVRGQTLRYFDEFSRHKLLDLLGDLRLAGDLSPCAIHARCSG
                     HELNHELLREIEKHTHSQKELER"
     gene            complement(5410..>6175)
                     /locus_tag="PLT86_11615"
     CDS             complement(5410..>6175)
                     /locus_tag="PLT86_11615"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOT37300.1"
                     /translation="PSTTRGSFLSPQAIVASTANVDKSAYVGAFVTIGNGTTVGERAV
                     IRDGVRIGEKVRVGAGTVIEENAVIHDRVRLGDRVLVGSGTVIGSVGFGFVAVEGQQK
                     RFPHVGTVVIEDDVEIGANCTVDRGTLGATRIGRGSKLDNLIHVGHNVQIGKGVLVAA
                     QCGFSGSSRIGDGVQMGGQAGVAGHISIAAGARVGAKSGVMKSVSGTVAGHPARQLIQ
                     QRRAEARLMHLEELVVRVSALEKALSERPCKNHLED"
BASE COUNT         1258 a         1894 c         1788 g         1235 t
ORIGIN      
        1 cgatatccag ccttgacaca cgccctgtgg cggctctgtt gttgtacacg cggaaccctg
       61 caccgatgtc cgctcggata tcccgcgggt caacacgtct gtcgggttgc cagacgtacc
      121 ccgcgtcagc gaacaccacg aaaccgagtc ggtacacaag cacacgcacc ggcgaaaaga
      181 ttcggtcttc ggcattcagg acgattctgc ggttaccttc gaaggcgttc gcactgtaac
      241 ctcgcaaccc tcgatcgccc cccagaacaa gctggtaggg accatccatt cgctctccgc
      301 tgcgccagtt cacgttcaac gcaaaggttt gccacggaag tccggtgtag taatgatgca
      361 ggtacgctgc cacctcacgg tttgaccatg cactccttcc ggaaccctgg tcgtcggcaa
      421 ggcgagtgct gacctgaagg cgtgctgcgg tgacgtgttc cccgcgaacg gcgcaccatt
      481 cggcgaatgc ccctgcgagc ccgtaaggat tgtgtgtcgg ttgggaaggc agaccgaatc
      541 ctgtggtaag acctatgttg gtacccgatt cgacgtcctc gacggtgtcg taatcgtcaa
      601 ggttgcgaag tttgtgaaat gcgctggtcc attgcctgct tatagtgaaa gatggttcat
      661 acgcaacccg tgagacgcgg attgtgtctc tgggcaacca cgtggcgaag agcaaggggg
      721 tgtccgtgtg cctcgtattc cgcaacctga gtgcggcctg caatttggtg cgcttggagg
      781 cttctcccca cgcgtgggcc acccaggcat acgtgtctgt cgtcgtaacg ggaagctccg
      841 cgacaggcac cttgtcgcgc caaagacgca tcctgccggt gaaatggtag aactcgactc
      901 cgcccgcgtt gcgcgtgcgc gtggtggtaa atggacgcgc cagcgcagtc atgaactcgt
      961 ttccgaggta cgcgcgcgat gcagaaacct gaagggcgaa acgcgagcct atgagccttg
     1021 ggtccttgta ctgaaaaagc cattccgttt ccgttgttcc gcgtgaacgg tttcgttccc
     1081 gttcgtactg cgccgcgatt tccttcccga aacccaacag gttcttctct gtgaccagcg
     1141 ctccaattct gtccgatccg ccaccgcttt cccaaatcca ccctgcggcg aggctgaaat
     1201 tgtcgctggt gcgcacaaga acccgtacgc cgtttgagtc tgcgggatag gcttcgatcg
     1261 tagcttcgtt gatgaacgag tacgctcgaa ggttccgttc ggactcatcc agagatttct
     1321 gccgcaaggt atcgccgacg gcgaagagga gttcgcgctc gacgacgtgt ggtttcgttt
     1381 tgatgtggag cgcgttgagc gtggcgtagg tccacctcga caaccgtctc cgcgtatgca
     1441 ctgcttcttc ccaccacggc ggaaccacgt ctccgggtac gacgtccacc tcagttatca
     1501 cgcggccctg gtgaaacgcc gtggaatcca cgaccgggga gtcctgtgga aacgccctgg
     1561 atgcgcagaa aaacgacatt gccagcacaa gcgccgtatt catcactgct gaatcccggg
     1621 gtgtgggacg tcgttcgccg tttcgatcgc cagcttgaca acccggttcg ctactccggg
     1681 tgtgcccagt ttcgtcctga tttctctggc cttttccacc cagaaatccg gtggccgctc
     1741 agtaaccgtt gccaacgccg attcgattcg agaagcttcc gcgtcctcct gaacacattc
     1801 agggtacgcg tttgagcaga gcagaatgtt ggggagccct atccatctgg tgtgaacgag
     1861 gcacctcgct acgctgtacg tcaaccagtc ggtacggtac gcaatcaccg tcgggatccc
     1921 tgccaaggcc gcttcgaggg tggctgttcc cgcgcaggca atacatgccc gggcttcgtt
     1981 caggagttgc gccagaggtg aggtggtgac aggcaacggt gcccgacctg ctcgggaaag
     2041 tgcggtccgg atccacccgg catggtgctc cgtcggtgcc cgaaacacgc aaggcacgcc
     2101 caggcgtctg ccgacgtcgg caaaaaggac ggataggcgg tcaatctcgg atttcctgct
     2161 tccggggagc aaaatcagca agtctccctc tcgccgttgt tcctgaggtt cgggaagctc
     2221 atccacgagg ggatgtccct cccagatgac gcgatcggtc tccattggag gtgcgaagta
     2281 cgcacgttcc cagtcgaaca acgttacgac tcgcgcgcaa aggtgagcaa ttgtccgtac
     2341 gcgcgacctt ctccaagccc acacctgagg cgggctgaaa tacaccgtcg gtacgcgcga
     2401 ttgcctgacg aagcgcagaa agggaatgtg gaaatcgggc gcgtcaatga ctatggagac
     2461 gtcaggtggg tgagcacgga attgacgtac gcttcgagcc atcagggagc agtactcatg
     2521 aaagcgcaca aatggctcgg cgaatcccat gacgccgtcc ggcgcagggg taccgtctgc
     2581 ccccgcatca cgcataagag gccccgagat tcccccaacc ctggcgttcg gagccagacc
     2641 ggcgatggcg cgtacgaggg ccgcacccca ccggtcgccg gaaggctcaa gagctgagat
     2701 ccagaagttc attgcctgtt ccgtcgaagg ttgttccgta cgaaacttga taccaccgaa
     2761 acaagcagaa atgcgagcac agactggtac gtgcgccgtt cgctgcatag cgcggttcga
     2821 acgccgaacc ggtgggttat gacagggatt ccgcggcggt acactctccc ctgttcccac
     2881 gcgacaatgg gcgcatagaa aaggaacgca aggaccggaa acacaaacgg caaccatcgt
     2941 tttcccgctc ccgaaaacag gacaagccct tcagagagta ctccatttgc caggtagagg
     3001 gggtggcgga aatacgcgta agggccgccg atggtccgga acgccggccc atccccgcgg
     3061 gatcgagatt gctctccgat gtacagaact gcccacagtc tgatggcaag gccggtcaac
     3121 accaacagcg cacctgaaac gagccgacct cctctgattc tggaggagag tgccaccgta
     3181 accgcaagag gaacggggag cacgtccctg tgcctgaaaa ggaatctacc aagagccgcc
     3241 agtctggcca ttttaccgga tgacgcctct ctgtgacctt tccacgaacg caacaaaacg
     3301 gtcgcgctcc acgctgggct cgagttcgct ggagcggctc agggcctgtg cagtgttgag
     3361 accggagcga aaaaacagcc ggtatattgc cctcaaccgg cgaaactgct ctgcggtgaa
     3421 cccgtgcctt ctcagaccga ccacgttcaa cccgtaaggg cggagcggtt ctccggcggc
     3481 cagaatgtag ggtggaacgt cctgcacagc gcggaagcca cccgctacgt aactgtaggc
     3541 gcctatccga acaaactggt gtacgggaac gagcgcgctt attgtggcga aatcctccac
     3601 cacgacatgg ccgccgagcg tggctccgtt aacaaggatg acgtcgtttc ccactgtgca
     3661 gtcatggccc acatgaacgt atgccatgag aagacatctt gcgccgattc gtgtggttcc
     3721 atcgcctcct cggtcctgga gaaccgagcc gctgttgagg gtagtgaact cccgaatgac
     3781 ggtatcatca cctacgatca ggaacccttc atgcggcggt tggggcttcg tctggggaat
     3841 cgtgccgagg caggcgtggg agaacacctg aaccctcgcg cccaaccgaa cgcctgacgc
     3901 gacatacgcg tatgaaccga tcgtgcaacc atcattcgca atagcaccgg ccttgacaat
     3961 ggcaaacggg gccaccgtga cacccgaacc cagttgtgcg ttttcctcca caacggcgcg
     4021 ggtatccacg tggttcacgg ctgctcctcc cctcgaacac gcagaaccgc agtcagttca
     4081 gcctccgacg cgacttcccc gttgacccgc gcctcccctt ttagcacgaa aagctcgcgc
     4141 cgttgtttca acacagtgat gtcaagaagc aattggtctc ccggcctgac gatgcggcgg
     4201 aatttcgcgc catctattcc gcgaaagaag acgtcgagtt tcgtcttttg acgatccacg
     4261 gcaagtctgt ggaacagcag tactgctcca gtctgagcga gcgcctccaa ctgaagcaca
     4321 cctggcatta cgggttccga tggccagtgt ccggcaaaat acggttcatt gatcgtgacg
     4381 ttcttgaggc ccgtgatacc gtcttcccgg atactgacga tcctgtctac cagaagaaag
     4441 gggtaccggt gtggaatccg gtccatgatg tcgcgaatat cgagcactgt gggttcatca
     4501 gcggtggcgc tcatcgttcc aactcctttt gcgaatgcgt gtgtttttcg atttcgcgca
     4561 gcagctcgtg attcagttcg tgcccgctgc accgcgcatg tatcgcacac ggggaaagat
     4621 ctcctgccaa cctgagatca cccagaaggt ccagcagctt gtgtcgcgaa aactcatcaa
     4681 aatatctcag cgtttgtcct ctcaccaacc ctttttccgg ccgcggctgg tcttccaccc
     4741 aggggggcag cgcgggatcg gagagcaggg acacgtcgtc aacccacaga agcgcgtttt
     4801 ctggagttcc acctttgatc atcccgagct tccgaagttt cagcgcatcg ctcaaggtac
     4861 agaaagtgcg ggcccgtgcc acatgctggc ggaagtccgt ctcctctgtc cattccacgt
     4921 gttgttcagg gccgtcgaag aagcggccgg agtactccac gcgccatgtc ttcgacggag
     4981 tcagcgtgat gcatgcgccc gcgtgggcga cgtgaatggg gggcagggga cccagggcaa
     5041 cggaggcacc cggtgtttcc tgcgttcccg caagaaaaaa cgcctcgcac cagatttccg
     5101 cgctcccgtc gccgatcggc agttcctccg aatccagctc gacgacgaga tcagtgatgc
     5161 cggtacccca gcatgcagca agcaggtgct ccggcgtcat caccgcccca tcgccctttt
     5221 ccagcctcgt gcaccgggag gacgaagcga ggttcgacgt tgctgcggaa atgcgcgggt
     5281 ttccgggaag gtccgaacgg acgaaggtga ttccgggccg atcagccgga aggagacgta
     5341 cggtcacgtc tgtgccgctg tggatcccgc gtccgcgtac ggtgacgcat ctgccgactg
     5401 tgtgtcgcat cagtcttcga gatggttctt gcagggacgc tcggagaggg ctttttccag
     5461 cgcagagacc cgcacaacca attcttcgag atgcatcagg cgtgcctccg cgcggcgctg
     5521 ctggatcaat tggcgggctg gatgccccgc gacggtgcct gacacggatt tcatgacccc
     5581 ggatttggcg cctacgcggg cgcccgcggc gatagaaatg tgtccggcca cgccggcctg
     5641 tcctcccatt tgtacgccgt ccccgatgcg tgagctgccc gaaaacccgc actgagccgc
     5701 cacgaggact ccttttccga tttgcacgtt gtgtccgacg tggataaggt tgtcgagctt
     5761 gcttccccgg ccaatccgcg tggctccgag ggttccgcgg tccaccgtgc aattcgcgcc
     5821 gatttcgacg tcatcttcga tgacgacagt accgacgtgc ggaaaacgtt tctgttggcc
     5881 ctccactgca acgaaaccga agccgacgct tccaatcact gtcccagaac ctacaaggac
     5941 tcgatcaccc agccgaacgc ggtcgtgaat tacggcgttt tcttctatca ccgttccggc
     6001 accgacgcgc accttttccc cgattcgtac gccgtcacgg atcacggcac gttcgccaac
     6061 ggtggttccg ttaccgatgg tgacaaacgc gccgacatat gcgcttttgt cgacgttggc
     6121 agtggaagcc acgattgcct gcggcgaaag gaaagatccc cgcgttgtcg aagga
//
LOCUS       DAOSOO010000189         6164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12830_length_6164_cov_4.707317, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000189 DAOSOO010000000
VERSION     DAOSOO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12830_length_6164_cov_4.707317"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLT86_11620"
     CDS             complement(<1..1563)
                     /locus_tag="PLT86_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37301.1"
                     /translation="MSKKTSHSENMEPLLLLDIHHSGYFGWGVWVSPDAIEHWTAETV
                     ERMMRYPLYKMGLNLGAHTYENSPELAPRIRSWMTSFPDRLFVTGGDYAQPTACVRSG
                     ESNLRHLLVGWEEIRRQLGVRVDIWTVSEPGNFAQLPQILKNLGYRGALLRIHGPGQL
                     GSPVPTSDSASVWWTGPDSTKILAVPEYSGDRLQKGDACTYSMWIMTRYRNTRATRGN
                     YTLDDLWNWKIAQERKGISPVVMSKDDDHNNQYTNNNLCMDSGHALAADTENDPRFRW
                     VTAEELFEAVPTPTCSLAADPNLFETRVSSFCDYGFMGNRDWVVDHRAEAELRMGDFS
                     RALAATLLGDVPGEKEMREAWKLHLGAQNHDVSLKSLSTVMYHLQHEASRIARGVSGR
                     VLRTIAGEIETGDGFGAVVVWNPLAFQRREYATVTLPKEMASKAALHLGETPIQWEVV
                     SEEAENVEMGFVADVPSLGYQTYAVRPGAQAETRCAARVEGRTITTPHFSVTFGERGG
                     IDVGTNPRRLVVA"
     gene            1764..2918
                     /locus_tag="PLT86_11625"
     CDS             1764..2918
                     /locus_tag="PLT86_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37302.1"
                     /translation="MTSRERVRAAFQHRQPDRTPIFEREIKHPTDNAILGRPAIHPYN
                     WPEFMRVWAREGWRVAMERYADDEFEILRLLEFDMIRLVPNPPSSAPRPVELDTFTFQ
                     QGDSVLRYHPASGVVEDVNARTRDLDRWENDFRANVEKEYVPPSLSDDQLFVWRRIRR
                     HIADAGLDVAIYVSLYAIPVAALPPFALEWFVTAPELLDRYYEKQSQWIIDTARLFRM
                     EGAEIAGLGGDFAGDTGPVISPRAYRERVVPHIQRQSDGLHEMGYWTTNTTDGYLWDV
                     LPDFLERSHVDGYGEIDVAAGMDLARLKKEWGERFTFLGNLDIRWVLCEGTLEEARAE
                     MERCIVQGWGNGGHIIMTSNVVHEDVRPELYLAAIDRYREYFGLPPRKNA"
     gene            complement(2991..3986)
                     /locus_tag="PLT86_11630"
     CDS             complement(2991..3986)
                     /locus_tag="PLT86_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT37303.1"
                     /translation="MRVVVLGATGLLGSGITRVLASRGHTVCAVARGNRQVVLPEGVQ
                     FTPTDRRDRAAMEHLVKSFRPDAVVDCVAYTREDGIIDRDVFAGKVAHLIMVSTDFVY
                     KPSFQVLPITEDAPVRAGTAYSEGKVDCEEVLLRAQELKVTVIRPPHIMGPGGKLGSG
                     SIQGRDSSLIDRVRKGVPIVLIDAGIHLLQPLHVDDAGDAVNAILCNTACFGKVYNVC
                     GPVADLTRRYYEVIAETLGVPLQTLSIAGDIWVAARPDQVSFVRHRVYSLERIHTDTG
                     WFPAITMETSVRRTVADILARGEDVPYVPSQAEERLLAALSRHSAEIRGILASLG"
     gene            complement(3998..4438)
                     /locus_tag="PLT86_11635"
     CDS             complement(3998..4438)
                     /locus_tag="PLT86_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT37304.1"
                     /translation="MVNLKVILHPTDFSPLSGNALRYGVEFARRFGARLIVLHVLEYA
                     YSAISLAEEAVADAENELQKVLSPSQKEGLDVEMLLVRGKPCEEILRLAREREVDLIV
                     MGTHNRGTLEHALLGSTTEKVIRKAPCPVLTIRHPEHEFIQQGT"
     gene            4741..5853
                     /locus_tag="PLT86_11640"
     CDS             4741..5853
                     /locus_tag="PLT86_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HOT37305.1"
                     /translation="MEIDSRLIGVYRSVGGAIQAASAYSMERIPRTDPLTGVRILQLT
                     SYPVLSHTLYYHCPSITPDSKTLVFMTYNRAGRFSTPDAWRVNLDGTEIRPVTNREGL
                     AGFVISPDGKTVYCQQGGTLLAAPMENSPTGNIAYEVASIPDVVIGATLLGSITADGK
                     WYISTAFLTDGSTALVRYATDGSEARVLKVAEYLVHVQVDQSDAGRILYVAPPDEKGR
                     AMWVTDCDGGNPRTLNLRHSTGHFVWFGKTGRLLSTVNDPFGSIVSIAEGEDEPQLIA
                     KGGHFWHAAGSQDGKWVVSDTNWPDIGLILINTETGLWAPLCKAEASEGHPQWTHPHP
                     MFTPDGRHIVFNSDRTGIGQIYTVEIPESLRQQLSG"
     gene            complement(5842..>6164)
                     /locus_tag="PLT86_11645"
     CDS             complement(5842..>6164)
                     /locus_tag="PLT86_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37306.1"
                     /translation="TIPVVAELSGGVPGPVHAGLAPDVCSGARAVEISTKAETTHAQN
                     PNRPSSRRSGDLSDLRTKSPRKDSRREIRRVSFASPLCDRSVRAARQAGGTEKVLVAC
                     VSTR"
BASE COUNT         1271 a         1860 c         1736 g         1297 t
ORIGIN      
        1 ggcaacgacc agccgacgtg gattagtgcc cacgtcgatg ccacctcgtt caccaaatgt
       61 caccgagaaa tgcggagtcg tgattgtccg tccctcgacg cgcgcggcgc aacgtgtctc
      121 cgcctgcgca ccggggcgga cggcgtacgt ctggtagcca agggatggca cgtccgcaac
      181 gaaccccatt tcgacgtttt ccgcttcctc ggacaccacc tcccactgaa ttggcgtctc
      241 accaaggtgc agcgctgcct tgcttgccat ctctttcggc agagtaacgg tggcgtactc
      301 tctccgctgg aacgccagtg gattccatac gactacggcg ccaaacccgt cccccgtctc
      361 aatctcgccc gcaatcgttc tcaaaacccg accagacaca cctcgtgcga tccgcgaggc
      421 ctcgtgttgg aggtggtaca tcactgtgct cagacttttg agggatacgt catggttctg
      481 cgcaccgagg tgaagcttcc acgcctcacg catttctttc tcaccaggca catcaccgag
      541 aagcgtggcc gcaagcgccc gggaaaaatc acccatccga agctctgctt ccgccctgtg
      601 gtcgaccacc cagtcgcggt ttcccatgaa cccgtaatcg cagaacgaac tcacgcgcgt
      661 ttcgaaaagg ttcggatcag cggccaacga acacgtggga gtcggaacgg cttcgaacag
      721 ttcctccgcc gtgacccacc gaaaacgagg gtcgttttcc gtgtccgcgg cgagcgcgtg
      781 gccggaatcc atacaaagat tgttgttggt gtactggttg ttgtggtcgt cgtccttcga
      841 catgacaacc ggcgagattc cctttcgttc ctgcgcgatc ttccagttcc acaggtcgtc
      901 cagcgtataa ttgcctcgtg tggccctcgt attcctgtac cgcgtcatga tccacatgga
      961 gtacgtgcaa gcgtcgcctt tttggagccg gtcgcctgag tattcgggaa ctgccagaat
     1021 cttcgtggag tcgggcccag tccaccatac agaagcggaa tccgaagtcg gcaccggact
     1081 gcccaattgg ccgggcccgt ggatacgcag caacgcaccg cggtatccaa ggttttttaa
     1141 tatctgaggt agctgcgcga agttccccgg ttcgctgact gtccagatat ctactctgac
     1201 tccaagctgg cggcgaattt cctcccaacc gacaagaagg tgacgcagat tcgattctcc
     1261 cgaacgaacg cacgcggttg gttgcgcgta gtcgccgccg gtgacgaaaa gccggtcggg
     1321 aaatgacgtc atccaactgc gaatcctcgg tgccaattcc gggctgttct catacgtgtg
     1381 cgcaccgaga ttcagcccca tcttgtaaag ggggtaccgc atcattcttt ccactgtttc
     1441 ggctgtccaa tgttcaatcg cgtcgggtga aacccataca ccccagccga aatacccgga
     1501 atggtgtatg tccagaagca ggagcggttc catgttctcc gaatgggatg tctttttcga
     1561 cacgggagat cctccgattg cagtaatcct gtggggtgtg aacacagagc aagccgcgaa
     1621 cgcagattgg gaacaaacga gtccggcgag acggccttga ccgtttcaaa atcgcccgta
     1681 acttactatg gacaaaaccc accggtacgt cgtgagccag acctcgcgac ggtaagcttg
     1741 cttcacgacg caaggtctcc ctcatgactt cacgagagcg tgttcgcgca gcgttccagc
     1801 acagacaacc ggacagaact cccatcttcg aacgcgaaat caaacacccg acagataacg
     1861 cgatcctggg aaggcctgcc atacacccgt acaactggcc ggagttcatg cgcgtctggg
     1921 cgcgcgaggg gtggcgcgtt gcaatggagc gttatgcgga cgatgagttc gagatactcc
     1981 ggctgcttga attcgacatg atccgcctcg tcccgaatcc tccctcttct gcgccgaggc
     2041 cggtggagct cgacactttc accttccaac agggagattc ggttctccgc taccacccgg
     2101 cttccggcgt cgtcgaggac gtcaacgcga ggacacgtga tctggatcgc tgggagaacg
     2161 atttccgtgc gaacgtggag aaggaatacg tgcctccgag tctgagcgac gaccaattgt
     2221 tcgtctggcg gcgaatccgc aggcacatcg ctgacgccgg gctggatgtc gcgatctacg
     2281 tctcgctcta cgccatccct gtagccgcgc tgccgccctt tgcgctggaa tggttcgtaa
     2341 ccgcaccgga actcttggac cgctactatg agaaacagtc gcagtggatc attgacacgg
     2401 cacgcctttt ccgaatggaa ggcgccgaaa tagcgggtct cggcggcgat ttcgcaggcg
     2461 acaccggtcc cgtaatctcg ccgcgtgcgt accgggaacg agtagtcccg catatccagc
     2521 ggcaatctga tggcttgcac gaaatgggat actggaccac caacaccacc gacggatacc
     2581 tgtgggacgt cctgcccgat tttctcgaaa gatcacatgt ggatggctac ggcgaaatcg
     2641 acgtggcggc cggaatggac ctcgcccgtc tcaaaaagga atggggggag cgttttacgt
     2701 ttctcggcaa tctggatatt cggtgggttc tctgtgaggg gacgctcgag gaagcacgag
     2761 cggagatgga gcggtgcatt gtccagggat ggggaaacgg cggccacatc atcatgacga
     2821 gcaatgtggt gcatgaggac gttcgcccgg agctctacct cgccgcaatc gaccggtatc
     2881 gcgagtattt cggtttgcca ccgagaaaga acgcgtgaac catctgagtt cagcgaccgc
     2941 tgttgtctgg acacattctc agggcgcggt acgaagcctc gggtatccgt ctatcccaac
     3001 gaggcgagta tccctctgat ctctgccgag tgccttgaaa gcgccgcaag gagccgctct
     3061 tcagcctgcg atggtacgta cggcacgtct tccccacgcg caaggatgtc tgccaccgtc
     3121 cgacgcacgg aggtctccat agtaatcgcc gggaaccaac ccgtgtccgt gtgtatcctt
     3181 tccaggctgt acacccggtg ccggacgaag ctgacctggt caggtcgcgc ggcaacccag
     3241 atatcaccgg cgattgacag agtctggagg ggaactccga gcgtctccgc aatgacttcg
     3301 tagtaccgcc gggtcaagtc cgcaaccggg ccacacacgt tgtagacttt cccaaaacag
     3361 gcagtgttgc agaggatcgc attcaccgcg tctcccgcgt cgtccacgtg aagaggttgg
     3421 agcaggtgga tgcctgcgtc aattaggacg atgggcacac ctttccgcac acggtctatg
     3481 agtgaagaat ctctgccctg aatcgagccg ctgccgagtt ttccacctgg tcccattatg
     3541 tgaggagggc gtatcactgt gaccttgagt tcttgcgcgc gtaagagcac ctcctcgcaa
     3601 tccacctttc cctcgctgta cgccgttcca gcgcgaaccg gtgcgtcttc agtgattgga
     3661 agaacctgga aggaaggctt gtacacgaag tccgtactca ccatgatcag gtgcgcgact
     3721 tttcccgcga acacatcacg gtcaatgatc ccgtcctctc gcgtgtatgc tacgcaatca
     3781 acgacggcat ctggtcggaa ggatttgacg aggtgttcca tcgcggctcg atcgcgcctg
     3841 tcggtgggag tgaattgcac tccctccggt agcacgactt gcctgttgcc ccgcgcaacc
     3901 gcgcaaaccg tgtgtccgcg cgacgcaaga accctcgtga ttcctgagcc caggagcccc
     3961 gttgccccaa gtacaacgac gcgcatggtg ttgcccttca agtaccctgt tggatgaact
     4021 cgtgctccgg gtgacggatt gtcaaaaccg ggcagggagc ttttctgatg actttctccg
     4081 ttgtgcttcc cagcaacgca tgttcaagag taccccgatt gtgggtgccc atgactatga
     4141 ggtcgacctc ccgttcgcgc gccaggcgga ggatttcttc gcaaggcttt ccgcggacaa
     4201 gcaacatctc aacgtccaga ccctccttct gagacgggga gagcaccttc tgcaactcat
     4261 tctccgcgtc tgccacggcc tcttcggcga gcgaaatcgc cgaatatgca tactccagaa
     4321 cgtgcaacac gatcagtctg gccccgaacc tcctggcgaa ttcgactccg tagcgaagcg
     4381 cgtttccgga taatgggctg aaatcggtcg ggtggagtat caccttcagg ttcaccatgg
     4441 cacgcctccc ttcccgttga tgaatctgtt gtacatcaca ctgtgggcct accggtgttt
     4501 tgcagcggca cacgcggttg ctcaagcatt attcgcgggt gtgccgagtt gtacaccgaa
     4561 atatcggttc gtggcggccc ccgtcaatct ctccccctct ccgccaattt tgacgaagaa
     4621 agaaaaatcc gtattctatc aatcgttcat tctgtttcgt tctgatgcaa atgggacggg
     4681 ctctccgtcc tgttctttcc gcccttcgcc gcatccgcac accccagaaa agggaaagca
     4741 atggaaatcg actcgcgatt gataggcgtc taccgttccg tgggtggcgc aatccaggcc
     4801 gccagcgcat acagcatgga acggattccg cgaacagacc ctcttacagg cgttcggata
     4861 cttcaactga cgtcctatcc cgtcctcagc cacacccttt actaccattg cccttccata
     4921 acgccagact cgaagacact cgtcttcatg acgtacaatc gagcgggaag attttccacg
     4981 cctgacgcgt ggagggtgaa cctcgatgga acggaaatcc gtccggtgac gaatcgggaa
     5041 ggtcttgcag gatttgtgat ctcgccggat ggaaagacgg tctattgcca gcagggagga
     5101 actcttcttg ccgctccgat ggagaactcc ccgactggca acattgcgta tgaagttgcc
     5161 agcatcccgg acgtggttat cggcgctact ctgctcggaa gcattacggc cgatggaaaa
     5221 tggtacatat cgaccgcgtt tcttacggac ggaagtaccg cgctcgttcg atacgccaca
     5281 gacggaagcg aagcgcgggt tctcaaagtt gcagaatacc tcgttcacgt acaggtggat
     5341 cagtccgacg ccggacgcat cctttacgtc gcacctcccg acgaaaaagg gcgcgcgatg
     5401 tgggtgaccg attgtgatgg gggaaacccg cgcacattga accttcggca tagcaccggc
     5461 cacttcgtct ggttcggaaa aacagggcgc ctgctttcca ccgttaacga cccgttcggc
     5521 tccatcgttt cgattgctga gggcgaggac gagccgcagt tgattgccaa gggaggacac
     5581 ttctggcatg cggccgggtc acaggacggc aaatgggtgg tctcggacac aaactggccg
     5641 gacatcgggt tgatcctgat taatacggaa accggcctct gggcgccgct gtgcaaagcg
     5701 gaggcgtcag aggggcatcc tcagtggaca cacccgcacc cgatgttcac gcctgacgga
     5761 cggcacatcg tattcaattc ggaccgcacg gggatcggcc agatttacac cgtcgagatt
     5821 ccggagagtc tgcgccaaca gctatcgggt tgacacgcag gcgaccagca ctttttcggt
     5881 accgccggcc tgtctcgcgg cgcgcacgga tcggtcacat aatggagatg cgaagctgac
     5941 acgccttatc tccctgcggg agtcttttcg gggggatttt gtccgaagat cgctcaggtc
     6001 cccgctacgc cggctggacg gacggttcgg gttctgcgcg tgggtggttt ctgccttcgt
     6061 gctgatttcg accgcacggg ctcccgaaca gacgtcgggg gccaatccgg catgcaccgg
     6121 ccccggaact cctccggaca gttctgcaac caccgggatt gttc
//
LOCUS       DAOSOO010000190         6156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_12857_length_6156_cov_5.316833, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000190 DAOSOO010000000
VERSION     DAOSOO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12857_length_6156_cov_5.316833"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLT86_11650"
     CDS             complement(<1..810)
                     /locus_tag="PLT86_11650"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOT37307.1"
                     /translation="MTDTIVALATPSGEGGIAIVRLSGTRAISIADSLFRAPRRLADA
                     PTHTLLYGRLALNDSAASPGSGVFDEVLVSVMRAPRSYTREDVVEINCHGGIRLADEV
                     IRACVSLGARRAERGEFTERAFLNGRLDLAQAEAVLDLVKARTKDALSAAYYELNGGL
                     SARINALRDRLTASLARIETGLEFDEEDLGVGDPRAESAALREILSEISALVGTYARG
                     RLIAEGASVAIIGPPNVGKSSLLNAILGEDRAIVSHIPGTTRDLVEGEADLD"
     gene            complement(875..2602)
                     /gene="yidC"
                     /locus_tag="PLT86_11655"
     CDS             complement(875..2602)
                     /gene="yidC"
                     /locus_tag="PLT86_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT37308.1"
                     /translation="MERNTVIALVLIILIWILWPKWIKWTSPETQPVVPAALPADSSR
                     GPSSIPDSIVRSAPRPVVAASDTTRVRAIAHADSVPVRTFTIITDKYEAVLSNEGAVL
                     RKWSLKDYHTPYGAPVQLVAPGGHGISVSVGSEGKEIDLREEVFVPDTVFQMAHLTGK
                     DSLAVGFTCTLPNGRRVFKRYVFYGDRYRVAFAAGSADSLTAERMVLSWEGAMPSTEG
                     DFRREAGSMFVNAYVGGSLEKLHTLDKTETSSFTGQTDWVGVRNKYFLAAFAPTSTDR
                     WDTRLFGSGVKDTSLRYSWRIAPFDLTKSTVTGFLYLGPLDVDILTAEGHNLERAADL
                     GWSVLRPISKLILWFFLSAYRFIPNYGWVIVVFSVLIKILLHPLTKKSYESTSKMQKL
                     KPLMDEIREKYKNDQQRLNQEMIKLYKEHGFNPLGGCLPMLLQMPIIFAIYAVIGNNI
                     EFRQAPFIWWVTDLSIPDSVYTLPFHIPLYGSHVTILPILMAISMFFQQKLTVTDPKQ
                     QALVYMMPVIMLFVFNNLASGLVLYWFLFNIFSSAHQYWLMKKQNGGEITPKTAPVPV
                     KNVVRPRKH"
     gene            complement(2618..2830)
                     /gene="yidD"
                     /locus_tag="PLT86_11660"
     CDS             complement(2618..2830)
                     /gene="yidD"
                     /locus_tag="PLT86_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT37309.1"
                     /translation="MLVWAAIGVIRIYQSLFSPLLGASCRFFPTCSSYGIEALSRHGF
                     FRGGWLTLKRILRCHPYHPGGYDPVP"
     gene            complement(2799..3134)
                     /gene="rnpA"
                     /locus_tag="PLT86_11665"
     CDS             complement(2799..3134)
                     /gene="rnpA"
                     /locus_tag="PLT86_11665"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT37310.1"
                     /translation="MRRETETTRWIRTVREEIPARRGSFLVAWVDKSSQRESPVVIVG
                     RTVGKAHERNRVRRRIREALRACVANPAGIIVVARTRARAADYWNLLNELEGLLAESD
                     ARMGGDRSD"
     gene            complement(3155..3955)
                     /locus_tag="PLT86_11670"
     CDS             complement(3155..3955)
                     /locus_tag="PLT86_11670"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOT37311.1"
                     /translation="MADYLLRENAPLTEQEWAKVDELVTSVASRLLVGRRVLPLFGPL
                     GAGVQTVPVDTFDLASEEGIRRSGRVFVVPRMIAREFILGWQDIETGRSGVLPLELSP
                     AAAAASACALAEDTLIFRGDAESDGILNAEGCLGTKMSDWATEGAFNDILAAVQKLQE
                     NGITEKPALVVSPAGYALMQKPFGNTGALELAFIRELMPAGVFQTPVLKPSEAVVIAT
                     GPQNADLAVGVDLSVGYVDQVDMDHRFRVLETLALRVKRPQAICTITR"
     gene            complement(4013..4945)
                     /locus_tag="PLT86_11675"
     CDS             complement(4013..4945)
                     /locus_tag="PLT86_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT37312.1"
                     /translation="MTDNEVVELLAHDMSMEHQAILQYLFAAWVIGGEVGPSIESVAR
                     DEMRHFKYFGLAIAELGGKPNMEGPAFQRLTDPTAIVLANIASEDEAVRVYTQHRDLI
                     PDPRIKKLYDRIIEEEMYHGQKFQRMVPKVEGMPPVEFHEPQSPQQETLFRFLEEDIA
                     GEYAAILRYLHQSFVVADGKLGNAIEDRAIDEMKHMGWMAEHKIDSGGEPVLQPAPVQ
                     YSTELSEIFQYNIQLEKGAEERYQRHIDGYEDPDLQRVWAHIKFQEEHHTDDFENRLG
                     DLNRAPEAQAATPPEASNVKPKTSVGSLFGKPQS"
     gene            complement(5019..5531)
                     /locus_tag="PLT86_11680"
     CDS             complement(5019..5531)
                     /locus_tag="PLT86_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37313.1"
                     /translation="MRVSPQSILLAAVFLFASSGSHAEVQSPNEARASIPADCEAGRV
                     CAMNDGVLDPVLASLLSDMGGLPPRARIRVFTPADIMAATNFDPLTISTAAGTWIVIA
                     QTGERGPVHWARASITPQGIMVKRGVFFPSRKAYAYSLARSAAVQFGICGGEDQSTGA
                     SFPVFMGNLP"
     gene            complement(5671..>6156)
                     /locus_tag="PLT86_11685"
     CDS             complement(5671..>6156)
                     /locus_tag="PLT86_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT37314.1"
                     /translation="AGTRFVMFSFDSVPCSVIICHDERYPELVRLPVLAGAQIIFYIS
                     CESDITAETKIAPYRAQIQARAMENSVFVVHSNAPMGNVRMRNGKPRGEAAASNGHSR
                     IIKPDGNIVAEASIWGEDVLVADLDMSLATRHLALRSLESPLLRSWWEAGLRLVPPPT
                     R"
BASE COUNT         1278 a         1827 c         1753 g         1298 t
ORIGIN      
        1 atcgaggtcg gcttcgcctt cgaccagatc gcgtgtggta cccgggatat gtgacacgat
       61 agctcggtcc tccccgagta tcgcattcaa gaggctggat tttcccacat tgggcgggcc
      121 aatgatggcg acgctcgcgc cttctgcaat gagtctgccc cgggcatagg tgccaacgag
      181 agcgctgatt tccgaaagga tttcccggag tgcggcgctt tcagcgcgtg gatcgcctac
      241 gcccaggtcc tcttcgtcaa attcaagtcc cgtttcgatt ctcgcgagcg aagcggtcaa
      301 acggtcgcgc agggcattga tccgggcgga aagaccgccg ttgagttcgt aatacgccgc
      361 agacaacgcg tctttggtgc gcgccttcac gaggtccaaa acggcttctg cctgcgcaag
      421 gtcgaggcgc ccgttgagga acgcgcgttc cgtgaactct cctctctccg cgcggcgagc
      481 gccgaggctg acacacgccc gaatcacttc gtccgcgagt cggattcctc cgtggcagtt
      541 gatttcgacc acgtcttccc gcgtatagct ccgaggtgcg cgcattacgg aaacgaggac
      601 ctcatcgaac acgccgctgc ccggtgatgc agcggagtcg ttcagtgcaa gcctgccata
      661 gaggagagta tgggtagggg catctgcaag gcgccgcggg gcacgaaaaa gggagtcagc
      721 tatcgaaatc gcacgagtgc cgctcaacct gacgatggcg atgccgcctt cgccggaagg
      781 ggttgcgaga gcaacaatcg tgtcggtcat ggcaaacggt ctgtcatgga atgtggtttc
      841 cggcggtgcg ccgagcgccg gaaccacgtt cgagctagtg tttgcggggt ctgaccacgt
      901 ttttcaccgg aaccggcgcc gttttcggag tgatttcgcc tccgttctgt ttcttcatca
      961 gccagtattg gtgcgccgac gagaagatgt tgaacaggaa ccagtacaac acaagtcccg
     1021 aggcgagatt attaaacaca aacagcatga tgacgggcat catgtacacc agcgcctgct
     1081 gcttgggatc cgtgacggtc agcttctgct ggaagaacat cgaaatggcc atcagaattg
     1141 gcagtattgt cacatgactg ccgtaaagcg ggatatggaa tggcaaggta tacacagaat
     1201 ccgggatgga aaggtcggta acccaccaga tgaacggggc ctgcctgaat tcaatgttgt
     1261 tgcctatcac cgcatagatt gcgaagatga tgggcatctg gaggagcatt ggcaggcatc
     1321 cgcccagcgg gttgaacccg tgctccttgt acagcttgat catttcctgg ttcaaccgct
     1381 gctgatcgtt cttgtatttt tcgcggattt cgtccatcag cggcttcagc ttctgcattt
     1441 tgctcgtgct ctcgtagctc tttttcgtca acggatgcag aagaatcttg atcagaacag
     1501 agaaaacgac gattacccac ccgtaattgg gtatgaacct gtaagcggaa aggaagaacc
     1561 agaggatgag tttggaaatc gggcggagaa cgctccagcc gaggtcagcg gcgcgttcca
     1621 gattgtgccc ctcggcggtg agtatgtcaa catcgagcgg ccccaggtac aggaatccgg
     1681 ttaccgtgct ttttgtcaaa tcgaagggtg cgatgcgcca ggagtagcgc aggctcgtgt
     1741 ccttgacgcc acttccgaaa agccttgtgt cccacctgtc cgtacttgtc ggcgcaaaag
     1801 ccgcgaggaa atacttgttt cgaacaccca cccaatcggt ttgccccgta aacgacgagg
     1861 tttccgtctt gtcgagcgtg tggagttttt ccagggatcc gccgacgtag gcgttcacga
     1921 acatgctccc tgcctctcgt cggaaatcac cctccgtcga aggcatggca ccttcccagg
     1981 atagaaccat ccgttccgcg gtcaaggaat ccgcggagcc cgccgcaaat gcaactcggt
     2041 acctatcccc ataaaaaaca tagcgtttaa aaacacgccg cccgttcgga agcgtgcagg
     2101 tgaacccaac ggcaaggctg tccttgccag tcagatgcgc catctggaac accgtatcgg
     2161 gaacgaaaac ctcctcccgc aggtcgattt ccttcccttc ggaacccact gacaccgaaa
     2221 tgccgtgtcc gcccggagcc accaactgca caggcgcacc gtacggggtg tggtaatcct
     2281 tcaatgacca cttccgcagc acggctcctt cgttggaaag aacggcctcg tatttgtcag
     2341 tgataatggt gaaggtccgg acgggcacgg aatctgcgtg agcgatggcg cgcacccggg
     2401 ttgtatcgga ggcagcaacc actggacgtg gtgcagaccg gacaatcgag tccggaatcg
     2461 aactcggacc ccgagacgaa tccgcaggca gtgctgcagg aacgacgggc tgtgtctccg
     2521 gcgaggtcca tttgatccac ttcggccaaa gaatccagat aaggatgata agcaccaatg
     2581 ctataacagt attacgttcc acgatcagca attcctctca cggtacgggg tcgtacccgc
     2641 ccgggtggta cgggtgacaa cgcaaaattc gtttgagtgt gagccacccg ccacggaaga
     2701 atccgtgacg ggagagggct tcgataccgt atgacgagca cgttggaaag aacctgcagg
     2761 aagctcccag aaggggggaa aacagggact ggtagatcct aatcactccg atcgccgccc
     2821 atacgagcat ccgattcggc aagaagaccc tccagctcat tcagcaagtt ccagtaatct
     2881 gcggcgcggg ctctggttcg tgcaacaacg atgatgccgg ccggattcgc cacacacgca
     2941 cgcagggctt cgcgaatcct gcggcgtact cgattccgtt cgtgagcctt tccgacggtc
     3001 ctgccgacga tcaccaccgg cgattcccgt tgcgaagact tgtctaccca tgcgaccaga
     3061 aaagaaccgc gccgtgcggg aatctcctcc cgcacggtgc ggatccaacg cgtcgtttcc
     3121 gtctcgcggc gcacgcaggg cttcccgccg cccgttaccg ggtgatggtg cagattgcct
     3181 ggggccgttt cacccgcagc gccagcgttt ccagtacgcg gaagcgatgg tccatgtcga
     3241 cctggtctac ataccccacc gaaagatcaa caccaaccgc aagatcggca ttctgcggac
     3301 cggtggcgat taccacggct tccgacggtt tgaggacggg tgtctggaac accccggcgg
     3361 gcatgagttc gcggatgaac gcgagttcca atgctcccgt gttcccaaac ggcttctgca
     3421 taagcgcata gcctgccggg cttacgacaa gcgctggttt ctcagtgatg ccattctcct
     3481 gaagcttctg gaccgcagcg aggatgtcat tgaacgcgcc ttcggtagcc cagtcactca
     3541 tcttcgttcc gaggcagccc tctgcgttca gaatcccgtc ggattccgca tcacccctaa
     3601 agatgagcgt gtcttcggcc agtgcgcagg ccgaagccgc ggcagccgcg gggctcagtt
     3661 ccagcggaag aacaccgctg cgcccggttt cgatgtcctg ccacccgagg atgaattccc
     3721 tcgctatcat gcgaggcact acaaacactc gtcctgatct cctgatccct tcttcggagg
     3781 cgaggtcgaa tgtgtctacc ggaaccgtct gcacgccggc cccgagaggg ccgaacagag
     3841 gcaggacccg acgaccgacg agcaatcggc ttgccacgct ggtgacgagt tcgtctactt
     3901 tggcccattc ctgctctgtg agcggagcgt tctcacgcaa aaggtagtcc gccattttcc
     3961 ttccccttgt ttcgagttct gttcacgtat gaaaagactt actcgcgcaa aatcacgact
     4021 gcggtttgcc aaacaacgat ccgacggagg tttttggctt cacgttcgaa gcctccggtg
     4081 gtgtggccgc ctgggcttcc ggcgctctgt tgaggtctcc aagccggttt tcaaagtcgt
     4141 cagtgtggtg ctcttcctgg aacttgatgt gcgcccacac cctctgaagg tcaggatctt
     4201 cgtacccgtc aatgtgccgt tgataccgtt cctcggcgcc cttttcaagc tgaatgttgt
     4261 actggaatat ctccgaaagc tccgtcgagt actgaacggg tgccggctgc aacaccggct
     4321 ctccgccgga gtcgattttg tgttccgcca tccagcccat gtgcttcatc tcatcgatag
     4381 cgcggtcttc aattgcgttt cccagtttgc cgtccgcgac cacgaaggac tggtgcaggt
     4441 accgcagaat cgccgcgtac tcgccggcaa tatcctcttc gagaaatcgg aagagggtct
     4501 cctgctgcgg agactgaggc tcgtggaact ccacgggcgg catcccttcg actttgggca
     4561 ccatgcgctg gaatttctga ccgtggtaca tctcctcttc gatgatcctg tcatacagtt
     4621 ttttgatccg cgggtccggg atcagatccc ggtgctgcgt gtaaactctg actgcctcat
     4681 cttcggaggc aatattggcg agaacaatgg ccgtgggatc ggtgaggcgc tggaaggcgg
     4741 gtccctccat attcggtttt cccccgagtt cggcaatggc caggccgaag tacttgaaat
     4801 ggcgcatctc gtcccttgcg acgctctcga tcgagggtcc gacctccccg ccgataaccc
     4861 atgctgcaaa caagtattga agtatcgcct gatgctccat gctcatgtcg tgggcaagca
     4921 actccactac ttcattatct gtcatgacag tgcccctttc gctgtcgttc ccggctatta
     4981 acgatcttca acccaatttg accgggatcc gcggtgcctc aaggaaggtt tcccatgaat
     5041 accgggaaac tcgctccagt cgactggtct tcccctccgc aaatcccgaa ctgtacggcc
     5101 gcgctccgtg ccagggagta cgcgtacgcc ttgcgcgacg gaaaaaagac ccctctcttg
     5161 accatgatcc cttgcggtgt tatcgaggct ctcgcccagt gcaccggccc acgttcgcct
     5221 gtttgagcga tcacgatcca ggtccccgca gcggtggaaa tcgtaagcgg atcgaaattc
     5281 gtcgcggcca tgatgtctgc gggtgtgaac acgcgaatcc gcgctcgcgg cggcagtcct
     5341 cccatatcgg aaagaaggct tgccagaact ggatcgagta ccccgtcgtt catcgcacaa
     5401 acgcgacctg cttcgcagtc tgcggggatg ctcgcacggg cttcattcgg gctttgcacc
     5461 tctgcatgcg atccggagga cgcaaagaga aacacggcgg caaggaggat agattgcgga
     5521 ctgaccctca ttgcgtcgca ccctttctcg agaacccgtg gaaccaggtc tcttcaaaga
     5581 tacgaaaagc gaaccagcgt tccatggaaa acggagcccg gagaccttca tgatggacag
     5641 ggatgttgat cctgttaggc ctcaggtggc ttaccgggtt ggaggtggaa cgaggcgcag
     5701 acccgcctcc caccacgaac gcaggagagg ggattcgagg ctgcgcaacg caaggtgccg
     5761 cgtcgccagg gacatatcca gatcggcgac caggacatcc tccccccaga tggatgcctc
     5821 ggcgacgatg ttcccatccg gtttgatgat ccggctgtgc ccgttggacg cggccgcttc
     5881 cccgcgcggt tttccgtttc gcatccgaac gttccccatg ggagcgttcg agtggaccac
     5941 gaagacgctg ttttccatcg ctcgggcctg gatctgggca cggtacgggg cgattttcgt
     6001 ttccgctgtg atgtcagact cgcaggagat gtagaaaatg atctgcgcgc ccgcaagcac
     6061 gggcagccga accagttccg ggtaccgttc gtcgtgacaa atgatcacag agcatggcac
     6121 cgaatcgaag ctgaacatca cgaaacgggt tcccgc
//
LOCUS       DAOSOO010000191         6076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13110_length_6076_cov_5.190002, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000191 DAOSOO010000000
VERSION     DAOSOO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13110_length_6076_cov_5.190002"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1388
                     /locus_tag="PLT86_11690"
     CDS             54..1388
                     /locus_tag="PLT86_11690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT37315.1"
                     /translation="MRKNRPLCCALLLIVASLLPFRTSFAAEEVVDRILAVVERDVVL
                     QSEVDEYITLQMIQAGRSIQSLSAPERDQLRCQILQAMVDDRILVAKARADSVVVTLQ
                     QVEDALRQQLDNIKGQFPTEEAYREQLVKEGTTERDLRAKLRLQMERYLLREKLTQTY
                     GQKITVTFNEMERFYNQYRDSLPVVPASVTIAHITRVTKPGDSALVAARRVLDAARER
                     LAAGEDFASVARALSQDPGSAQFGGDLGFFGRGQMVPEFETAAFSLDSGQVSGPVLTE
                     YGLHLIQNLGFRGEQVHARHILARAVFTPADRQATVDTLQSVYRRLQAGQDFGELARI
                     YSMDQSVRQTGGRIGPVDPSALPPAFGRVLATLSVGETSQPFESDPGTFHIVRLLERT
                     REHAMNMNDDRRQIEEMVRQRKLQTRINEILEQERGRMYVDVRLPGCFPSAR"
     gene            1394..1693
                     /locus_tag="PLT86_11695"
     CDS             1394..1693
                     /locus_tag="PLT86_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOT37316.1"
                     /translation="MPAAADTKGAVLRMDSVLFRLRLVSSREQAKRACENGIVELNGK
                     VAKPSSSVRSGDFIRVRFTDEDLRFTLIELPRKSMSKTSARSCYAVTASNAVERG"
     gene            1697..2467
                     /locus_tag="PLT86_11700"
     CDS             1697..2467
                     /locus_tag="PLT86_11700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37317.1"
                     /translation="MAFLRRDVIEKPPYDQLAALYDYVMRHVDYVEWADYIEQLFRWC
                     GANPHKITEAACGTGALALILADRGYSVSGYDLSEAMISVAKAKARARDENLPQFAVA
                     DMKSLPHGEADVVLCMYDSINYCSNTGELAATFRSFRKAIRAEGIAIFDVTTEANSLR
                     YFRDYRYRERHDGYMISRRSVYHPETRTQTNEFVFEGPDLPGRVKEMHRQQIYSLKEV
                     LESIPAFEWSVLGAFDGYTLDPPDDDTERIHIVMRALG"
     gene            2483..3160
                     /locus_tag="PLT86_11705"
     CDS             2483..3160
                     /locus_tag="PLT86_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT37318.1"
                     /translation="MGDTPFLAFDAVTLQGRRAPIVEDVSLSLAAGGFGVLFGPTGGG
                     KTTILRLAHLELRPTVGRVKVGDISSDHLNAGEAAMARRAIGMVYQDSRLLPDRSASE
                     NIILPLQIAGMATGMARRRALEVLGQVGLAGKRSISPRYLSGGELQRLALARALAMDP
                     HLLLADEPTGNLDSDTAEEIMTLIRRINARGTTVLLATHDLKLIGGYGYPCWRVEDGH
                     AVQEEPR"
     gene            complement(3291..3596)
                     /locus_tag="PLT86_11710"
     CDS             complement(3291..3596)
                     /locus_tag="PLT86_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT37319.1"
                     /translation="MPDHFDTVDHVAVVVDDIGQAVNWYSSRFRCEVAYQDDTWALVR
                     FANISIAFVLPGHHPRHVGIHREDIEQFGNVGVHRDGVRFVYLEDVSGNVVEVVKRP"
     gene            complement(3620..4435)
                     /locus_tag="PLT86_11715"
     CDS             complement(3620..4435)
                     /locus_tag="PLT86_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37320.1"
                     /translation="MSAFDVSTILKSVSAMQEKPPRLWWSTDEASQMRSRLLRDNRYA
                     ESVEAHAESVIRDPAVFAREPAWHVSQCEPLLSLAYAAWLFQRERYASLASRLMDRAA
                     SAESWDAPDEFSPKPVAVALTLAKAIDLLAAFTTDDQLERYDIAAREKCFARLPGILK
                     SRGMTDQERMVWCSDSACAALGTLSPSGLLIQEVARAASCLMAALEKKSDVRALPVVR
                     IGRALSRRSDDVARLMRDQGLNLAIPEPNPFAVFDCEVLALLELPVACIPSAA"
     gene            complement(4475..5116)
                     /locus_tag="PLT86_11720"
     CDS             complement(4475..5116)
                     /locus_tag="PLT86_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase family protein"
                     /protein_id="HOT37321.1"
                     /translation="MLVEQIRTGGDRNFSYLVADETRRVCAVVDPSFAPDLVAEKAAT
                     LGFRVEYVLVTHDHFDHTNGNARLKELTGAQVVMHRMSEENCDVRVDNGDVLPLGALS
                     ITVIHTPGHTRGHACYLVEDAVFTGDTLFVGKVGGSDFGEGARQEWESLHTKLLSLPD
                     NTRVFPGHDYGVRPFSTIGDERRSNPFLLQPDFPSFLKLKMNWAEYKKLHGIK"
     gene            5399..6073
                     /locus_tag="PLT86_11725"
     CDS             5399..6073
                     /locus_tag="PLT86_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HOT37322.1"
                     /translation="MARSILALFSRSPWGAIQEHMRSVNQCVQLLDPLMDAVMAGNHA
                     RTGELAKEISALEHAADEVKNELRDHLPRSIFLPVAREDLLEILSTQDSISDAAEDVG
                     MIVTMRKMEAQSDLRDLLDPLVQKTLESVSCYSQVIGEIDRLVDASFVGKEAQRVMDL
                     IRQVSTLEHEADLAQEALSRRLFEIEDRLKPGALFMWIEIIRRLGSVANKAEKAANRL
                     RMFLAT"
BASE COUNT         1255 a         1716 c         1843 g         1262 t
ORIGIN      
        1 tgagtgcttc gggaatgcaa tgatccgaat cttctctgtg gaaaggttgc tttgtgcgga
       61 aaaacaggcc tttgtgttgt gcgttgctgc tgatcgtggc aagtctgctg ccctttcgaa
      121 ccagttttgc cgcggaagag gtcgttgacc gcattcttgc ggtggtcgag cgcgacgtgg
      181 tattgcagtc cgaggtcgac gaatacatca ccctgcagat gatacaggcg gggcgcagca
      241 tccagtctct gtcagccccg gaacgcgatc aattgcgttg ccagatactt caggcgatgg
      301 tggacgaccg gatactcgtg gcgaaagcgc gcgcggacag cgtcgttgtt accctgcaac
      361 aggtagaaga cgcgcttcgg caacagctgg acaacatcaa aggccagttt ccgactgagg
      421 aggcctaccg ggaacaactg gtcaaggaag gaacaacgga acgcgacttg agggcgaaac
      481 tgcggcttca gatggaacga tatctgctca gagagaaact gacgcagacc tacgggcaga
      541 agatcaccgt gacattcaac gaaatggagc gtttctacaa ccagtaccgc gatagccttc
      601 cagtggtgcc ggcttcagta accatcgccc acataacacg agtaacgaag ccaggggatt
      661 ccgcgcttgt tgcggcgcgc cgggtgctcg atgcggcacg tgagcgtctg gctgccggag
      721 aggacttcgc ttcggttgcg cgggctcttt cacaggatcc gggaagtgcg caattcgggg
      781 gtgatcttgg ctttttcggg cgaggccaga tggttccgga gttcgaaacg gcggcgtttt
      841 ccctcgattc cggtcaggtg agtgggccgg ttctcacgga atacggactg cacctgatcc
      901 agaatctcgg atttcgcggc gaacaagtgc acgcgcggca catcctggcg cgcgcagtgt
      961 ttacccccgc ggatcgccag gcgactgtgg acacgcttca atcggtgtac cgccgcctgc
     1021 aggcggggca ggatttcggc gaactggcgc ggatctactc gatggatcag agcgtgcggc
     1081 agaccggggg gcgcatcggg ccggtggatc cctccgcgtt gccgccggcg ttcggtcggg
     1141 ttctggcgac gttgagtgtc ggcgaaacca gccagccgtt tgagtctgat cccggcacgt
     1201 ttcacatcgt gcgcctgctc gagcgcaccc gcgaacacgc gatgaacatg aatgacgatc
     1261 gaaggcagat tgaggaaatg gtgcggcagc gcaagctgca gacgcgcatc aatgagatcc
     1321 tcgaacagga acgtggtcgc atgtacgtgg atgtgcggct gccaggctgc ttcccttcgg
     1381 cacggtaatg gcggtgccgg cggctgcaga tacgaagggc gcagttctgc ggatggactc
     1441 cgttctcttc cgtctgcgcc tcgtttcatc ccgcgaacag gcgaaacgcg cgtgtgaaaa
     1501 cggaatcgta gagctcaatg gcaaggtggc aaaaccgtca tcgtcggttc ggagtgggga
     1561 tttcattcgg gtgcgtttca ccgacgagga tttgaggttt acgctgatcg aattgccgcg
     1621 caaatctatg agcaagacat cggcgcgaag ttgttatgcc gtcactgcca gcaatgctgt
     1681 ggagagaggg tgattcatgg cattcctgcg gcgcgacgtg atagaaaaac ccccttatga
     1741 ccagctcgct gcattgtacg actacgtgat gcggcacgtt gattacgtgg aatgggcgga
     1801 ttacatagag cagttgttcc gttggtgcgg agcaaaccct cataaaataa ccgaagcggc
     1861 gtgcggaacc ggggcgctgg ctctgatcct ggcagaccgc gggtactccg tttccgggta
     1921 cgatctgtct gaagcgatga tttccgtggc aaaggcaaag gcccgtgcga gagacgagaa
     1981 tcttccgcaa tttgccgtgg cggatatgaa gagccttccg cacggcgaag ctgacgtcgt
     2041 gctctgcatg tatgacagca tcaattactg cagcaatacg ggagagcttg cggcaacgtt
     2101 ccgcagcttt cgcaaggcta ttcgcgctga aggtatcgcg atctttgatg ttaccacgga
     2161 ggcaaattcg ctccggtatt ttcgagatta ccgctatcgc gaacgtcacg acgggtacat
     2221 gatctcgagg cgcagtgtgt atcaccccga aactcgcaca cagaccaatg agttcgtttt
     2281 tgaagggccc gacttaccgg ggcgagtgaa agaaatgcac cggcagcaga tttactcgct
     2341 caaagaggtg ctggagagca taccggcgtt cgaatggtcg gtgttggggg catttgacgg
     2401 gtacacactc gatccgccgg acgatgatac cgaacgaatc cacatcgtta tgcgtgctct
     2461 gggttgagcg acaggggagt gcatgggcga caccccgttt ctcgcgtttg atgccgtgac
     2521 tcttcaaggg cgccgggcac ccattgttga ggacgtatcc ctgtctcttg cggcgggtgg
     2581 atttggagtc cttttcggcc ctaccggcgg cgggaagacg acgattctca ggctggctca
     2641 cctcgagctc cgacctacag tggggcgcgt gaaagtcggg gatatttcct ccgaccacct
     2701 caacgccggc gaagccgcca tggcgcgtcg tgccatcggg atggtgtacc aggattcccg
     2761 cctccttccg gacaggtctg caagtgagaa catcatactg ccgttgcaga tcgccggaat
     2821 ggcgaccggg atggcccggc gtcgtgcact cgaggttctc ggtcaggttg gattggcggg
     2881 aaagcgcagc atttctcctc gatatttgag cggaggagag ctgcagagac ttgctcttgc
     2941 ccgtgctctg gcgatggatc cccaccttct cctcgcggac gaacccacag gcaatcttga
     3001 ctcggacacc gcggaagaga tcatgacact gattcgtcgc ataaacgcgc gcgggaccac
     3061 cgtgcttctt gccacccacg atctcaagct gatcggggga tacgggtacc cctgctggcg
     3121 agtggaggac ggccacgctg tacaggaaga gccgcggtga cggttgaagg ctgcgcgtac
     3181 tgaatcagcg cgtttcgagt ggatttcggt tgagcgtggt cgaggttggt ggtgtggcca
     3241 gccgcccagg ctttcaggtg accccctgcg gcaagtccga acagctcact ttacggacgc
     3301 ttgacgactt ccaccacatt tccggaaacg tcctccaggt agacgaacct cacgccgtcg
     3361 cggtgaacgc ctacgttccc gaactgctct atgtcttccc ggtggatgcc gacgtgccgc
     3421 ggatggtgcc cgggaagcac gaaggcgata ctgatgttgg cgaaacgaac caatgcccac
     3481 gtgtcgtctt gatacgcgac ctcgcatctg aaccgcgagg aataccagtt cacggcctgt
     3541 ccaatgtcgt ccaccaccac ggcgacgtgg tccaccgtat caaaatgatc gggcatcggc
     3601 acggtctccc ttgcgcatgt cacgcggccg aggggatgca tgcgacaggc agttcgagaa
     3661 gcgcgaggac ttcgcaatca aacaccgcga acggatttgg ctcgggtatt gccagattca
     3721 gcccctggtc gcgcatgagc ctcgcgacgt catcacttct tcgggaaagc gctcttccga
     3781 tccgaacaac aggaagcgcc cgtacgtcgc tttttttctc cagcgccgcc ataaggcagc
     3841 ttgctgcccg agccacctcc tgaatcaaca gacctgacgg agacagcgtt ccaagagcgg
     3901 cgcatgcgct gtctgaacac cacaccatcc gctcttgatc tgtcattccg cgcgatttca
     3961 ggattccagg cagtcgggca aaacactttt cccgggccgc gatgtcgtac cgctcgagct
     4021 ggtcgtccgt tgtaaacgcc gcaagcagat cgatggcttt tgccagcgtc aacgctaccg
     4081 cgacaggttt gggagagaac tcgtcgggcg cgtcccatga ctccgcagaa gcggcgcggt
     4141 ccatcaaccg gcttgcaagc gacgcgtatc gttcccgctg aaacaaccag gctgcgtacg
     4201 ccagggacaa cagcggctca cactggctga cgtgccacgc cggctccctg gcgaaaacgg
     4261 cggggtcccg gatgacactt tcggcgtgcg cctcaacgga ctcggcatac cggttgtcgc
     4321 ggagaagtct cgatcgcatc tgtgacgctt cgtcagtcga ccaccacaga cgcggtggtt
     4381 tctcctgcat cgcggaaacg gatttcagaa ttgtgctcac gtcgaatgca gacatatcgt
     4441 cgttcctgtc attgtacttc cgcttggctg ctcttcactt gattccatgc agtttcttgt
     4501 attccgccca gttcattttg agcttcaaaa acgaggggaa atcgggttgt agcaaaaacg
     4561 gattggatct gcgttcgtcc ccgattgtag aaaaaggccg gacgccgtag tcgtgcccgg
     4621 gaaagacgcg ggtgttgtca ggaagtgaca gaagtttcgt gtggaggctt tcccattcct
     4681 gccgcgctcc ttcgccaaag tccgaacccc cgaccttgcc cacgaacagg gtatcccccg
     4741 tgaacactgc gtcctctaca agatagcatg catgcccgcg tgtatggccc ggtgtgtgga
     4801 tcaccgtgat ggaaagtgcg cccagaggaa gtacatcgcc gttatccaca cggacgtcgc
     4861 agttctcttc gctcatacga tgcatgacca cctgtgcccc ggtcaactct ttgagccgcg
     4921 cgttgccgtt cgtgtggtca aaatggtcgt gcgtcaccag cacatattcc acgcggaaac
     4981 cgagtgtcgc ggctttctcc gccaccagat ccggcgcgaa ggaagggtcc accacagcgc
     5041 atacgcgccg cgtttcgtca gcaacgaggt aggagaaatt cctgtcgccg cccgttcgaa
     5101 tttgttccac cagcacgttg cagctccgga ttcgtcagac gattcccatt aaaaatgccc
     5161 gcgttcgcgt ccgtttccaa gaccagggac aagaagcgcg caccggtcgg gttctggaag
     5221 cccgcggatt acgcgctcag gtgcggccgc cacacgaact ggtgacgggg gcgtgctgag
     5281 cgtaacgcgg gcaatcggtc catgcgcatt cccgcgacgc cggtttgaga cacctcaacg
     5341 tgcgaagtac attggaattc ggcgttcaac cgggttcgga gttttggagg gcaagggtat
     5401 ggcacgttct attctcgcgt tattctcccg ctcaccctgg ggagccattc aggaacacat
     5461 gcgatcggtg aatcaatgcg tgcagttgct ggacccgctc atggatgctg tcatggcagg
     5521 caatcatgcc cgcacgggag agctcgcgaa agagatatcc gcgctggaac acgccgcgga
     5581 cgaggtgaaa aatgaattgc gcgatcatct cccgcgaagc atttttctcc ctgttgcgcg
     5641 cgaggacctt ttggaaatcc tttccacgca ggacagcatt tccgatgcgg cggaagatgt
     5701 cggcatgatc gtaacgatgc gaaaaatgga agcgcagagc gatctgcgag accttcttga
     5761 cccgcttgtt cagaagacgc tcgagtccgt gtcctgctac tcacaggtga taggcgaaat
     5821 cgatcgtctt gtggatgcgt cgttcgttgg caaggaagcg cagcgtgtga tggacctgat
     5881 tcggcaagtg agcacgctgg aacacgaagc ggacctcgcg caggaggcac tttcacgccg
     5941 gctttttgaa atcgaggacc gtctcaagcc cggtgcgctc ttcatgtgga ttgaaatcat
     6001 tcgccgactg ggcagtgtcg ccaacaaggc ggaaaaagcg gcgaatcgcc tccgcatgtt
     6061 tctcgcaacg taacgt
//
LOCUS       DAOSOO010000192         6062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13160_length_6062_cov_5.246213, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000192 DAOSOO010000000
VERSION     DAOSOO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_6062_cov_5.246213"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PLT86_11730"
     CDS             <1..1198
                     /locus_tag="PLT86_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT37323.1"
                     /translation="IFPERSIALRLSDPAGDYVPVPTSISRVVYDAPLKKGHARFCFN
                     VPSARLWSPETPHLYQLDLTLVDTGSEQPRDWWSHRIGLREFRVDGPRLLLNGKPLVV
                     KGVNTYEDYPVAHHSHNEAALGQDLDLLKWIGGNAFRSHVPAHRRRYELSDERGVLQL
                     VELPFYQWGRPLVQTDNPDALADAKGQLRELIKARKNHASVFMWSVSNECLVVPRSND
                     PQAVSLARMTAEGNIELVKLAHELDPTRPVVEVSNCWPGDPVHEHTDLPCVNVYVGSA
                     PHVNGLPVVAERIREKFEALRKDHPDKPILAGEWGSWCVRGVKTDYFPGEVFQAEKIR
                     LFWETMREEENFIGGFIWCFADYDVHRRFLWVYEYRCAYGLFDYHRRPKEAAYAVRRM
                     WTGKGN"
     gene            1198..3039
                     /locus_tag="PLT86_11735"
     CDS             1198..3039
                     /locus_tag="PLT86_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT37324.1"
                     /translation="MRPHISLNGLWKFCPAFDAISADQRWLDSDFDPKNPDLTPVTAG
                     AARGWIKPDYDDSRWLDITVPNSWNAAIRDLWSYEGHGWYRRTIHIPRAWKDKRVEFV
                     SEGANYRVALYVNGELAGEHEGGYNPFSIPIHRFLKFGARNLIAVICDNIPKPYRSPG
                     GQFGWWNHGGLYRDVSLRVTDTTYIDDVTVVTDVNGSHVDVGVTVEVACEAGEMTTRR
                     IEIELDDPSGKPVPLPSERNAQTVTLAGGIAKAAFTFALDGVLLWSPAEPNLYALRVK
                     LLEDGCTTDEWSHRIGLRTFRIDGTRLLLNGEPFLVKGVNRHEQYAGGTIHTPTHTEA
                     QISEDVDLIKWLGGNAIRQHYPNHRRLYELCDERGIVCMVENPLWQWGRPLVETDNAD
                     ALATAKRQLTEIIRALKNHASVFMWSVSNENLVKAKSDDAKVVALANQTIEGNKELVA
                     LAKKLDPTRPVVEASNEWPGDAVLCETDISAVNVYVGNPVPHVSGAPKIVELMKAKMQ
                     KLREEIPGKPILAAEFGEWTVRGLVQDYPPGENYQTAKLTALWKGFMEQPDIIGGFIW
                     VFADYDLHRRFLWAYEYRCAYGLFDIERKPKAAAYAIRELWTAGNGR"
     gene            complement(3143..4123)
                     /locus_tag="PLT86_11740"
     CDS             complement(3143..4123)
                     /locus_tag="PLT86_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT37325.1"
                     /translation="MRIAVVGTIVYDEIVTHRGDRKQSFGGITYNIAALESIIDEHTQ
                     LVPIAQVGYDRYEDVVRLYSSYRGVVMDGLRRMPDQKNAHVQLIYKSVKERTESMQNV
                     PPPLAPEQLELAASCDAVLVNFITGRELEAESFQRLGTRAKYLHLDIHNKIADWTPEG
                     RRVFVGLPEWREYVSRATTVQMNEFEIEQLLGRPVKGTKEYLAAAAEVLDAGPQGVMI
                     TLGPLGSVVAYRSGDAVYGTAWPAANLGEVLDTTGCGDSFSAGFVWSYLRRNDVLWAN
                     AAANIVGDVNCITPGIGNLTKAREMDRLVPEAFGELAKKLAAGWKGERLV"
     gene            complement(4216..5037)
                     /locus_tag="PLT86_11745"
     CDS             complement(4216..5037)
                     /locus_tag="PLT86_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT37326.1"
                     /translation="MYVGLLTAPFGKEPIDKVIEFAAENGFAGLEITAGPGAHIDTTR
                     FNANEAERVKELVAKSNLKISSLAYYVNICDGRAETSEHYLKVIDAAAMLGTDVVCGM
                     AGMPVQGKSKEDTIKEVVPKVYRPILDHAAEKGIKIAMENWYATNIQHLGLWDLIFDV
                     VPDKHFGLNFDPSHLMWQGIDYLGAVERYRDRIFHTHAKDTEIKQHVLRHVGYLAGGW
                     WRYVIPGFGEIDWGVYIERLYAIGYKGVLSIEHEDGAQGREEGFIRGLRHLKQWV"
     gene            5217..>6062
                     /locus_tag="PLT86_11750"
     CDS             5217..>6062
                     /locus_tag="PLT86_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37327.1"
                     /translation="MPAILTSEPHLFVDLNEVEERCNITRNFHTARKYGRHPVLKQDA
                     PWEKHPGMTASVIYDVEEKVFKAWYMAGFYEPGSKHVQCYAVSDDGIHWNKPALGLHE
                     ALGSKENNIVIPSSYHDGMDHWESMLKDPLDSEPARRYKALGWSSYDWDGPLSGIYTA
                     TSPDGLNWEHTPEPVFRFHPRPGRNDLGPVGDAQGLMIDTLKKRYVAFLRTGISRSLS
                     ISEDFVRWTAPRPFLQSLNEEETLYNNVGFVYGNQYLGFLTHFDRNAYHQTQTLQLLT
                     SRDGET"
BASE COUNT         1304 a         1743 c         1744 g         1271 t
ORIGIN      
        1 gatcttccct gagcgcagca ttgccctgcg cctgagcgac ccggcgggtg attatgtccc
       61 cgttccaacg agcatttccc gcgtcgtgta tgacgcgccc ctgaagaaag gccacgcgcg
      121 gttctgcttc aacgtccctt cggcgcgttt gtggagtccg gaaactcccc atctgtacca
      181 actggacctc acactcgtgg atactggatc tgaacaaccc cgggactggt ggtctcaccg
      241 gatagggctc cgcgagttca gagtggatgg tccccggttg ctgcttaacg gcaagccctt
      301 ggttgtgaaa ggcgtaaaca cctacgagga ttacccggtg gcgcaccact ctcacaacga
      361 ggctgcactg ggacaagacc tggatctctt gaaatggatt ggcgggaacg cgttccgttc
      421 acacgttccc gcgcaccggc gtcggtacga actttctgac gaacgaggtg ttcttcagct
      481 cgtcgaactg ccgttctatc agtggggaag gccccttgtt caaacggaca accccgatgc
      541 gctcgcggat gcgaaagggc aattgcgcga gttgataaag gcgaggaaga accacgcgag
      601 tgtgttcatg tggagcgtga gcaatgaatg cctcgtcgtc ccgcgctcga atgacccaca
      661 ggccgtttca ctcgccagaa tgactgcgga agggaacatc gaactcgtca agcttgcgca
      721 cgagttggat ccgacgcgcc cggtcgtcga ggtgagcaac tgctggccgg gtgatccggt
      781 tcatgagcat accgaccttc cgtgtgtcaa cgtgtacgtc ggttcagctc ctcatgtgaa
      841 cggccttccc gttgtggcag aacggattag ggagaaattt gaagctctgc ggaaggatca
      901 tccggacaag cccatccttg ccggtgaatg gggaagctgg tgtgtccggg gagtcaaaac
      961 ggattatttc cccggagagg tcttccaagc tgagaaaatc cggctgttct gggaaacgat
     1021 gagggaagag gaaaacttca tcggagggtt catctggtgc ttcgctgatt acgatgttca
     1081 ccgacggttt ctctgggtat acgaatatcg ctgcgcgtac ggattgttcg attaccaccg
     1141 caggcccaaa gaagccgcgt acgcggtccg caggatgtgg accgggaaag gaaattaatg
     1201 cgcccgcaca tttcgttgaa tggtctgtgg aagttctgcc ctgcgtttga cgcaatcagt
     1261 gcagatcagc gatggctcga ctccgatttt gacccgaaga atcccgatct tacgccggtt
     1321 accgcaggtg cggcgagggg atggatcaag cccgattatg acgacagccg gtggctggat
     1381 atcacggtgc cgaacagctg gaacgccgcg atccgcgacc tttggagtta cgaagggcac
     1441 ggttggtatc gtcgcacgat tcacatcccc cgcgcgtgga aagacaagcg ggtggagttc
     1501 gtttccgaag gagcgaatta tcgagtggcg ctgtacgtga acggcgaatt ggcgggagaa
     1561 cacgagggcg ggtacaaccc gttcagtatt ccgatccacc gtttcctcaa attcggagca
     1621 cgcaatctca tcgcggttat atgtgacaat attccgaaac cgtatcgctc accgggcggc
     1681 cagttcggct ggtggaacca cggaggcctg tatcgggatg tttcgctccg agtgaccgac
     1741 accacctaca ttgacgacgt caccgttgtt acggatgtca atggctccca tgtggacgtg
     1801 ggggtgacag tcgaagttgc atgcgaggcg ggcgaaatga cgacccgtcg gatcgagatc
     1861 gaactcgacg acccgtcagg taaaccggtc ccccttccct ccgaacggaa cgcgcagacg
     1921 gtgactctcg ccgggggaat cgcgaaagcc gcgttcacat tcgctctcga cggggtgctg
     1981 ttgtggtctc ccgccgaacc gaacctctac gcgctacgcg tgaaactgtt ggaagacggt
     2041 tgcacaacgg atgaatggag tcacaggatc ggtttgcgca cgttcaggat tgacgggacg
     2101 cgtttgctcc tgaatggcga gccgtttctc gtcaagggcg tgaaccgtca cgagcagtac
     2161 gccgggggaa ccatccacac gccgacgcac acggaagcgc agatctctga ggatgtggac
     2221 ctcatcaaat ggctcggtgg gaacgcgatt cgacagcatt accccaacca ccggaggctg
     2281 tacgagcttt gtgacgagcg cgggatcgtg tgcatggtgg aaaatcccct gtggcaatgg
     2341 ggacgaccgc tcgttgaaac ggacaatgcg gatgccctcg ccacggcgaa gcggcagctc
     2401 accgaaatca ttcgggcgct caagaaccac gcgagtgtgt tcatgtggag tgtgagcaat
     2461 gagaacctcg tgaaggcgaa atctgacgac gcaaaggtgg ttgctctggc aaatcagacc
     2521 atcgagggaa acaaggagct tgtcgcgctg gccaaaaagc tggatcccac acgcccggta
     2581 gtggaggcga gcaatgaatg gccgggtgac gccgtcctct gcgagacaga tatcagcgcc
     2641 gtgaacgtgt acgtcggtaa cccggtgccc cacgtatcag gtgcaccgaa gatcgtcgag
     2701 ctgatgaaag ccaagatgca gaaactccgg gaggagattc cgggcaagcc tatcctcgcg
     2761 gcggagtttg gagaatggac ggtgcgcggg ctcgtgcagg actacccgcc gggggaaaac
     2821 tatcagacgg ccaaattgac tgcgctctgg aagggcttca tggagcaacc cgacatcatc
     2881 ggcgggttca tctgggtgtt tgccgattac gacctccatc ggcgatttct ctgggcgtac
     2941 gagtatcgct gcgcgtacgg gcttttcgac atcgagagga agccaaaagc ggcggcgtac
     3001 gccatccgcg agctgtggac ggccgggaac gggcggtaag atcgcccaaa ggcgaaccag
     3061 aagcatcgca acaaacagag agggcgcggt taaccgcgcc ctctctgttt caatttcccg
     3121 ctccaacctc actgcatacc catcaaacca ggcgttcgcc cttccatcct gcggcaagct
     3181 tcttcgcaag ctctccgaac gcctccggaa cgagcctgtc catctcccgc gccttcgtca
     3241 gattgccaat tccgggcgtg atgcagttca cgtcgcccac gatgttggcg gctgcgttgg
     3301 cccagagcac atcgttcctg cggagatagg accacacgaa ccccgcggag aatgaatctc
     3361 cacatcctgt cgtatcgagc acttcgccga ggtttgccgc aggccatgcg gtgccgtaca
     3421 cggcatcacc cgatcggtac gcgacgaccg aacccagcgg cccgagcgta atcattaccc
     3481 cttgcggacc tgcgtccagg acttcggcgg cagcggcaag gtattccttc gtgcctttca
     3541 cgggccgacc gagaagctgc tcgatttcaa actcgttcat ctggacggtt gtcgcgcggg
     3601 aaacgtactc cctccattcc ggtagaccga cgaatacccg tcgcccttcc ggcgtccagt
     3661 cggctatctt gttgtgaatg tcgagatgca ggtatttcgc cctggtgcca agccgttgga
     3721 atgattcagc ttcgagttct cgtcctgtaa tgaaattgac caggactgcg tcgcaactgg
     3781 cggcaagttc aagctgttcc ggcgccagcg gggggggtac gttctgcatg ctctccgtgc
     3841 gctctttgac gctcttgtag ataagctgca cgtgagcatt tttctggtcc ggcatgcgac
     3901 gcaggccgtc catcaccaca ccacggtaag acgagtaaag gcgaacgacg tcttcgtagc
     3961 gatcgtaccc cacctgagca atcggaacga gctgggtgtg ttcatcgatg atggattcca
     4021 gcgccgcgat gttgtaggtg attccgccga acgattgctt ccgatcgcca cggtgtgtga
     4081 cgatttcgtc gtacacgatt gttccaacaa cagcaatacg catgtatcac cccgcactgg
     4141 tgagaaaagg ttggcatcac aaaacaagga gaggagcatc cgggtgctcc tctccaaaac
     4201 atcactatgg ccggctcaga cccactgttt gagatggcgc agacccctga tgaaaccctc
     4261 ttcgcgccct tgagccccgt cttcatgctc tatggagaga acgcctttgt aaccgatcgc
     4321 gtacagccgc tcgatgtaga caccccagtc gatctcgccg aatccgggga tgacataccg
     4381 ccaccaccct cctgccagat agccgacgtg tctcagcacg tgctgtttga tctctgtatc
     4441 cttcgcgtgg gtatggaaga tgcgatcccg gtagcgttcc actgccccca aatagtctat
     4501 cccctgccac atgagatggg acgggtcaaa attcagcccg aaatgtttgt ccggtacgac
     4561 gtcgaagatg aggtcccaga gcccgaggtg ctggatgttg gttgcatacc agttttccat
     4621 cgcgatcttg atgccttttt cagccgcgtg gtcaagaatc ggtcggtaca ccttcggcac
     4681 gacctccttg atcgtgtctt ccttggattt tccctgcacc ggcattccgg ccatcccgca
     4741 cacgacgtcc gtacccagca tcgccgctgc atcaatcacc ttgaggtaat gctcggatgt
     4801 ttccgcccgc ccgtcgcata tgttcacgta gtacgcgagg ctggagatct tcaggtttga
     4861 tttcgcgacc agttctttga cgcgttccgc ctcgttggcg ttgaaacggg tggtgtcaat
     4921 atgggcgccc gggcccgctg ttatctcaag accggcgaaa ccgttttccg ccgcaaattc
     4981 gatcaccttg tcaatgggtt ctttcccgaa cggtgcggtc agcaaaccga cgtacatctt
     5041 cgtctccttt gagtcatgca ggggaaccgg ctgaattctg gtctgttgcc aagagaaaat
     5101 acctcccgaa tgccggggaa tccaactgag cgacatgtcg ccagcttggt ttgcgaaaga
     5161 gattccacgt tgttcatatc cgtcaacgga aatccacccg aagcgagggt tttgacatgc
     5221 ctgccatttt gacctcagaa ccgcaccttt ttgtcgattt gaacgaagtc gaggaacgct
     5281 gcaacatcac aaggaacttc cacacggcac gcaaatatgg ccgccatcct gttttgaagc
     5341 aggatgcgcc atgggagaaa catcccggaa tgactgcgtc agtcatctac gacgtggagg
     5401 aaaaggtctt caaggcgtgg tacatggccg gtttctatga gcccggttcc aagcacgttc
     5461 agtgctatgc cgtttcggac gatggcatcc attggaacaa gcctgcgctg ggccttcacg
     5521 aagctctcgg ttcgaaggaa aacaacatcg tgatcccctc atcatatcac gacggcatgg
     5581 atcactggga gtccatgctg aaggatccgc tggattcaga gccggctcgg cggtacaagg
     5641 cgttgggatg gtcgagttat gactgggatg gaccgctcag cgggatttac accgccacct
     5701 cacccgacgg cctgaactgg gaacacaccc cagaaccggt tttccgtttc catccgagac
     5761 cgggccggaa cgatctcggg cccgtgggcg acgcacaggg gctcatgatc gacacactga
     5821 agaaacgcta tgttgcgttc ctgcgaaccg gcatatcacg ctcactcagt atcagtgagg
     5881 acttcgtgcg atggacggcc ccccgtccct ttctgcagtc tctgaacgag gaggaaaccc
     5941 tttacaacaa cgtcggattc gtgtacggca accagtatct cggctttctc actcactttg
     6001 accgcaacgc gtaccatcag actcagaccc tccaacttct caccagccgt gacggcgaga
     6061 cc
//
LOCUS       DAOSOO010000193         6053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13186_length_6053_cov_5.499333, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000193 DAOSOO010000000
VERSION     DAOSOO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13186_length_6053_cov_5.499333"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..258
                     /locus_tag="PLT86_11755"
     CDS             58..258
                     /locus_tag="PLT86_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37328.1"
                     /translation="MTDGTSDETPDVNLEGDLLTFPTTTGGVYVIADGNEAPSPNPVL
                     SGCAAVHAKEREGRWIGIPRMW"
     gene            complement(346..630)
                     /locus_tag="PLT86_11760"
     CDS             complement(346..630)
                     /locus_tag="PLT86_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT37329.1"
                     /translation="MSREELIAKAASEAGVSKKAAGEVLSALLGGITAALKSGDKVSL
                     VGFGTFAVGQRKARVGKLPGTKKEIKIPAAKVPKFKAGKNLKEAVAGKKK"
     gene            complement(876..3653)
                     /locus_tag="PLT86_11765"
     CDS             complement(876..3653)
                     /locus_tag="PLT86_11765"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOT37330.1"
                     /translation="MQPGEHALRVPCALRIACGLCEIAVLLLLWLPAPLRAQLPSTAT
                     GERFATFGGFPDSVSTDSVRYAADEIEYDLDTRVITLLGNARLSAAGMSLTAHRIRFL
                     TREDLLIAEGHAISPDSIIGRPEFSSGQEAFSGARMTYNLRTRRGLVEEGHSEVTEGI
                     YGGSVVKRTGERQVDVLNGVYTTCDDTPPHYQFEARQMRVLVGDKVIAKPIVFKVADV
                     PFFWLPFGVFFVDKDRRSGFIAPRMGENRYAGRFMRGLGYYVAPNDYVGGQAVLDIDE
                     RIGYEWRLRTDYALRYRFQGSIASDYSKYWDTPSQIGRRIWTVTAQHRQELTPRSSVA
                     ANINFTSTSSPYVTSSSTQASVLRQDFTSTVGYTQTWESGYSLRVDARRSQNLQNRQL
                     DVMLPALSISSGQRYFFPEPPPQRGPRAAAAPRQEPAWFRRLGYSWSWSGTNSQRRSP
                     SDRTIFDTWETTEQRGDSVIVYLLTITSTSYINSLYDGTYVVAVENGERIGEGYVQRT
                     SDSNALVLISRSAGLPDGYAIMSGNSLRVKEPDIAQEVLWTRKRILDGYTQTTSQNVG
                     LTLPLPTPRWLTLVPTFNWSASWKSRPDELNANDPQTQQTISGGVSSNATFYGVFPVH
                     LGPVSAVRHVVTPSASFNYVFSSRTRGGTYVFGGRSLGGDTSRIVDLGLRNVFQVKAR
                     TSGKERVLDQLLSVSTNARYNRDAKGRRWSDPQTSALFQPNNFISLRLSTVHTLYRAE
                     FDSTSARYVDHLRWTRPELRSITLSTSLRMAGGGRSPSALGNEGFVVQRQETFGGNPR
                     VPPRGAKQADRTTADWGAWQMNIRHTYQWARKEPGYTRAPEPVNQADISAEFVPWPNW
                     RVSATTTYDVRRNRRDGDALTLVRRLHCWEAQLDWTLRGPFAGYYFRIYLLDIPDIKI
                     ESASETMRYAR"
     gene            complement(3661..4080)
                     /locus_tag="PLT86_11770"
     CDS             complement(3661..4080)
                     /locus_tag="PLT86_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT37331.1"
                     /translation="MFSSTTFSVPTRRKNEMIDITGAVFDIVRQRGIKSGICTVFVPH
                     TTAAVTINENADPDVPRDILVAFERIVPRDGDYAHSEGNSPAHLKALLTGSSVTVAVE
                     TGNIQLGTWQGIFLCEFDGPRNRQVRVSVLCESAASS"
     gene            complement(4092..4475)
                     /locus_tag="PLT86_11775"
     CDS             complement(4092..4475)
                     /locus_tag="PLT86_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37332.1"
                     /translation="MLVVRAILLWIAMIPLAILNGTVRVYWINPVIGEHAGHIVSSVT
                     LSAIVFLLTWWFMRNAIPGPREAFLVGAGWVICTVLFEFVFGHYVMNHPWEKLLADYN
                     VFRGRTWVLVLLAEFFSPVLALRLR"
     gene            complement(4507..5502)
                     /locus_tag="PLT86_11780"
     CDS             complement(4507..5502)
                     /locus_tag="PLT86_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT37333.1"
                     /translation="MDDTNITSLKTGFDATMATARAFMESRAILTAVELDLFTAVCET
                     SSASSVAHKIGTDPRATEMLMNVLVTQGLLKKAEDRYALTPVAEKYLTSTSPDSARDA
                     IMHTVGLWNSWSTLTAAVRAGTSVAKRRRNAEETEAFIAAMHRNSLERASVVAGALGI
                     ARYRNMLDVGGGSGGYSITFARMNPALRSVAFDLGPVARIARRHIKDAGVADRVSVKR
                     GDFHSDPFGNGFDLVFLSAICHMNGPEENRSLFRKAFAALQPGGDLVIHDHILNADKT
                     APKAGVLFALNMLVNTAAGNSYSEEEYHAWLKETGFSRIRSVPLPGATGLVVGTR"
     gene            complement(5646..>6053)
                     /locus_tag="PLT86_11785"
     CDS             complement(5646..>6053)
                     /locus_tag="PLT86_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT37334.1"
                     /translation="MSYIGIILLVGIVVNNGIVLVDFTNQMMRLEHKELFEAAYEAGK
                     TRLRPVLMTALTTIMGMFPLSLGLGESGESWAPLARAVMGGLLFATLMTLIVVPVVYT
                     LFETMAIRFRAWRDRRTARKHPELVVEEGAQLM"
BASE COUNT         1200 a         1769 c         1824 g         1260 t
ORIGIN      
        1 gagggcgtgc gcgggtagga gtgccgtatt gttctgccgg acgaaggtgc gtggggagtg
       61 actgacggga cctccgacga gacccccgac gtgaatctcg aaggcgatct gctgacgttt
      121 ccaaccacga cgggcggggt ttatgtgatt gctgacggca atgaggcgcc aagcccgaat
      181 ccggtgcttt cagggtgcgc cgccgttcac gcaaaggagc gagagggccg atggatagga
      241 attccgcgga tgtggtgatg cgcgggacgg aaccgcccgg aactacaaaa gccctgcctc
      301 atggagacag ggcttttgta tacatcagac caagccgggc aggggttatt ttttcttgcc
      361 agccacagcc tctttgaggt tcttgccagc cttgaacttg ggaaccttcg ccgccggaat
      421 cttgatttcc ttcttcgttc cgggaagctt gcctacccgt gctttgcgct ggccgacggc
      481 aaacgtgccg aagccaacca gggacacctt gtcgccgctt ttgagagcgg cggtgatccc
      541 gcccaacaac gcgctcaaca cctcgccggc agccttcttg gacacaccag cttcgctcgc
      601 cgcttttgcg atcagttctt cacgggacat tggttcaccc cctaccgtgt agatggttca
      661 tttgcgttcc cgatgagtaa aatagaacgt caggagcgat ttgtcaacac aggagtatcg
      721 atttttcggt aattttccaa ggtttttttt catccgagcg cgactgggtc gcactgccat
      781 atcgttagat tctgtaatta ttaacactta catcacgctc aaaaatcccg cttctcggaa
      841 gcgttttttc ggtttttttt gtgctcccgt ggcgttcatc gggcgtaccg catggtttca
      901 ctggccgatt cgatcttgat atcggggatg tcaaggaggt aaatgcggaa atagtatccg
      961 gcaaagggac ccctgagcgt ccagtcgagt tgcgcttccc agcagtgcag gcgtctgacg
     1021 agagtgaggg catcaccgtc tctccggttt ctgcgcacgt cgtatgttgt ggtcgcgctg
     1081 acccgccagt tgggccaggg gacgaattcc gcggagatat ccgcctggtt gacgggttcg
     1141 ggcgctctcg tgtatccagg ttccttcctc gcccattgat aggtgtggcg aatgttcatc
     1201 tgccacgcgc cccagtcggc cgtggtgcgg tccgcctgtt ttgcgccgcg gggcggaaca
     1261 cggggattgc ctccgaaggt ttcctgccgt tggacgacaa agccttcgtt tccgagcgca
     1321 ctgggacttc ttccccctcc cgccattcgc agcgaggtgc ttagggtgat gctcctcaac
     1381 tccggacgcg tccagcgcag gtgatccacg tagcgcgccg aggttgaatc gaactcggcg
     1441 cgatagagcg tgtgaacggt tgaaaggcga agggagatga agttgttagg ctggaacagc
     1501 gcgctcgttt gcgggtcgct ccaccgcctg cccttcgcgt ctctgttgta ccgggcgttc
     1561 gtggacacgg acagaagctg atcgagaacc cgttcctttc ccgaagttcg tgctttcacc
     1621 tgaaagacgt tccggaggcc gaggtcgacg attctgctgg tatcgccacc taggctccgt
     1681 ccgccaaaga cgtacgtgcc gccccgcgta cgggaagaga acacgtaatt gaacgacgcc
     1741 gagggggtta cgacgtggcg gaccgcgctg accgggccca aatgaaccgg aaacacaccg
     1801 tagaatgtgg cgttgctgga gacgccgccg gagattgtct gttgcgtctg cgggtcgttg
     1861 gcgttcagtt cgtcggggcg gcttttccac gacgcagacc agttgaacgt cggcacgagg
     1921 gtcagccacc gtggcgtggg aagcggcaac gtcaacccga cattctggga agtggtctgc
     1981 gtgtagccat ccaggatcct cttgcgcgtc cagaggactt cctgtgcgat atcaggctcc
     2041 ttcacgcgga gggagttgcc ggacatgatc gcgtagccgt cgggaaggcc ggcagagcgg
     2101 gaaatcagca cgagggcatt gctgtcgctc gtccgctgaa catagccttc gccgattcgt
     2161 tccccgtttt cgactgcaac gacataggtg ccgtcataga gcgagttgat gtagctcgtg
     2221 cttgtgatgg tgaggaggta gacgatgacg ctgtctccgc gctgttctgt cgtctcccat
     2281 gtgtcgaaaa tggttctgtc ggaggggctt ctccgctggc tgttcgttcc actccacgac
     2341 caggagtatc cgagcctgcg gaaccatgcc ggctcctgac gaggggctgc cgccgcacga
     2401 ggcccgcgtt ggggaggcgg ttcggggaag aagtagcgtt ggccggaact tatggagagg
     2461 gccggaagca tcacgtccaa ctggcgattc tgaaggttct ggctcctccg cgcgtcaacg
     2521 cgcaggctgt acccggattc ccatgtctga gtgtatccga ccgtcgaggt gaaatcctgc
     2581 ctgagcacgg acgcctgggt tgagctggag gtgacgtagg gcgaggaggt gctggtgaag
     2641 ttgatgttgg cagccacgga acttcgcgga gtgagttcct gccggtgttg cgcggttaca
     2701 gtccagatgc gccgcccgat ctgggagggt gtatcccagt acttgctgta atccgacgcg
     2761 atggagccct ggaagcgata acggagcgcg taatccgtcc gcagccgcca ttcgtaccca
     2821 atgcgttcgt ctatgtcgag cacggcctgt ccccccacgt agtcgttggg agcgacgtag
     2881 taaccgagcc cacgcatgaa tcggcccgcg tatctgtttt cgcccatgcg gggcgcgatg
     2941 aatccggaac ggcggtcctt gtcgacaaaa aacacaccga acgggagcca gaagaagggg
     3001 acgtctgcca ccttgaacac gattggtttc gcgatgacct tgtcaccgac gagtacgcgc
     3061 atctgcctgg cttcaaactg gtagtgcggc ggggtatcgt cgcacgtggt gtacacaccg
     3121 ttcagaacat ctacctgcct ttcaccggtc cgcttgacaa cgctccctcc gtagatgcct
     3181 tccgtcactt cgctgtggcc ctcttccacc aagcctctgc gcgtgcgcag gttgtacgtc
     3241 atccgtgcac ctgaaaaggc ttcctgcccg ctggaaaact ccggcctccc gataatggag
     3301 tctggcgaga ttgcgtggcc ttccgcgatc agaaggtctt cgcgtgtgag gaaccgaata
     3361 cggtgcgccg taaggctcat cccggccgcg gacaggcgtg cgttgccaag gagagtgatc
     3421 accctggtgt cgaggtcata ctcgatttcg tcggccgcgt accgaacgga atcggtggac
     3481 acgctgtcag ggaaaccgcc gaacgtggcg aagcgctcgc cggtagcggt agaaggaagc
     3541 tgggcgcgga ggggagcagg taaccacagt aagagcagca cggcgatctc acacaatccg
     3601 cacgcgattc tcaaggcgca ggggacgcgg agcgcgtgtt cgccgggctg catcgcaatc
     3661 tcaggaggaa gccgcggatt cgcacaggac ggagactctg acctggcggt tcctcgggcc
     3721 gtcgaactca caaaggaata tcccctgcca cgtacccagc tggatgtttc ccgtttccac
     3781 ggcaacggtt acggatgatc cggtgagaag cgccttcagg tgcgccgggc tgttcccttc
     3841 cgaatgggcg tagtccccgt cccgcggaac aatgcgttcg aacgcgacga ggatatcgcg
     3901 gggaacatcc ggatctgcgt tctcgttgat ggtaaccgcc gcggttgtgt ggggaacaaa
     3961 gaccgtacaa atcccgctct tgattccgcg ttggcgaacg atgtcgaaga ccgcaccggt
     4021 gatgtcgatc atctcgtttt tccggcgcgt cggcacgctg aatgttgtgg aagagaacat
     4081 ggaaacgacc cctaccgcag gcgcagtgcg agaaccgggg aaaagaactc ggcaagaaga
     4141 acgagaaccc acgttctgcc ccgaaacacg ttgtagtcag ccagaagctt ttcccacggg
     4201 tggttcatca catagtgtcc gaacacgaat tcgaacagca ccgtgcatat gacccatcct
     4261 gcgcccacca ggaacgcctc tctgggtccg ggaatcgcat tccgcatgaa ccaccacgtc
     4321 agaaggaaca cgatggcgct gagagtaact gaagacacga tatgtcccgc gtgctcaccg
     4381 ataaccgggt tgatccagta gacgcggaca gtcccgttga gaatagccag cggaatcatt
     4441 gcaatccaga ggagaatcgc ccgcacgaca agcatcacgc acctcatgtt cgggaagctg
     4501 ttgcgttcac ctggttccaa caacaagccc ggtagcaccg ggaagcggca ccgaacgaat
     4561 tctgctgaaa cctgtttcct tcagccacgc gtggtactcc tcctcgctgt aggaattccc
     4621 ggctgccgtg ttaacaagca tgttcagcgc gaacagaacg cctgccttcg gcgccgtctt
     4681 gtccgcgttg aggatgtgat catgaattac cagatcacca cccggttgca aagcggcgaa
     4741 agccttccga aacagcgatc tgttttcctc cgggccattc atgtggcaga tcgcggaaag
     4801 gaacacaaga tcgaagccgt ttccaaacgg atcgctgtgg aaatcgcctc ttttgacgct
     4861 cacgcggtcc gccactcccg cgtccttgat gtgccgtcgc gcaattcgcg caaccgggcc
     4921 cagatcaaac gccactgacc tgagggccgg gttcatccgg gcgaaggtga tgctataccc
     4981 tccggaaccg cccccgacgt cgagcatatt ccgatacctt gcaataccaa gcgcacccgc
     5041 gaccacagac gcccgctcga gagagtttcg gtgcatcgcc gcgatgaagg cttccgtctc
     5101 ttccgcgttg cgtctgcgtt tcgctaccga ggtcccggcg cgcaccgctg ccgttaacgt
     5161 gctccacgaa ttccacaggc ctaccgtgtg catgatagcg tcgcgcgcgc tatcgggaga
     5221 agttgaggta aggtactttt cggcaacggg cgtcagcgca taacgatctt cggctttttt
     5281 gaggaggccc tgggtgacca gcacgttcat cagcatctct gttgcgcgcg ggtcggtccc
     5341 gattttgtgc gcgaccgagg atgctgatga tgtctcgcat actgccgtga agaggtccag
     5401 ttccaccgcg gtcaggattg cgcgactctc catgaacgcc cgcgcggtgg ccatggtggc
     5461 gtcgaacccg gttttcagcg atgtgatgtt cgtatcgtcc atcggtgtcg atcctctatc
     5521 cgcggatgcg gaccatgacc ttgtacatcc ccacaaagcg agggaaccac tcccaaaaaa
     5581 gagaagccgg agggccgtgc tgtttcagaa gcgaccctcc ggcctcgtac tcgaatgtaa
     5641 cgaagctaca tgagctgcgc accctcctcg acaaccagtt ccgggtgctt ccgggctgtc
     5701 cggcgatcgc gccatgccct gaaacggatg gccatggttt cgaacagggt gtacaccacg
     5761 ggcaccacta tcagggtcat aagtgtggcg aacaacaggc cacccatgac ggcgcgcgcc
     5821 agcggagccc aggattcacc tgattcgccc agcccaagtg aaagcgggaa catgcccatg
     5881 atcgtggtga gagccgtcat caacaccggc ctcaggcggg tttttccggc ttcatacgcg
     5941 gcttcaaaca gctccttgtg ttcgagccgc atcatctggt tcgtgaaatc caccagaacg
     6001 attccgttgt tcacgacgat cccgacgagc aggatgatgc cgatgtagct cat
//
LOCUS       DAOSOO010000194         5994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13373_length_5994_cov_7.399057, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000194 DAOSOO010000000
VERSION     DAOSOO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13373_length_5994_cov_7.399057"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PLT86_11790"
     CDS             <1..1410
                     /locus_tag="PLT86_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37335.1"
                     /translation="LGNGVIRTPAIDRLVREGTSFQRAYTPSPVCVSARCSMIYGQYP
                     LHTGCYDNAPMPQDGRTSFMGALTEAGYRTHGIGKCHFYPDRASLRGFQTRESQEEGV
                     ASPEQDDYLVWLRANGYEHLTDPHGVRGEMYYTPQPAQMPARLHPTQWIGDRATAFLS
                     EPERKREPWYLYASFIHPHPPFAPPVPWHKLYRAPLMPLPNVPAEYEALHIYINRYQN
                     RYKYRDQGIDQNLLRAIKAYYYGAVSFIDYQVGRILAALEETGQLENTLIVFASDHGE
                     YLGDYYCFGKRGMHDAAARVPLILRYPERFAAGAVCQKPASLVDIAPTVLAAAGARVT
                     DHELDGVDLSELASGACPREMVFSQFQKAEQGVYMAVDERWKYFYSAPDGREFLFDHR
                     VDPLETRNRVATPFMKDHGARMRAALISHLKAGGETAALDGDGWKQYPKREMPEDPDA
                     GLLIQDSPWGVLKIPGYSD"
     gene            1465..2865
                     /locus_tag="PLT86_11795"
     CDS             1465..2865
                     /locus_tag="PLT86_11795"
                     /inference="COORDINATES: protein motif:HMM:NF023980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HOT37336.1"
                     /translation="MLKRSFARRWVSTTLFILATTAGSMAAERQITNDSTQNCALDQN
                     HNFSPDGKWLVYDLRPNGGLQNCRSIEKVNVVTGERVRVYDGPDFVENMGPGIAAPSY
                     LPERNEVIFIHGPFTTTGLAYDMARRTGAIVPDDGSAMNDTSRITWADARDVTPPFTP
                     GALRGGTHRHDPGGPGGLWIGYTYNDQIMKRYGQQIGRDLDLRMLGLTKLGIPVSVDA
                     DPAHENRSGAGFSVVITEVFPKDELDREPGTDRIYRATDDQWVGTRGYRKPDGSWQLA
                     RAFIGHMRVRAADGSVRDHREVFIVDIPEDITRPGPAGPLEGTGTTFPAPPAGTSQRR
                     LTHTDSGCFGLVRSTSDGGMIAFLSRAADGTPQVFVVSPLGGEPRQITSFPGGASHPV
                     RWLPDDLHFVTVSEGRVYVVDAASGCARAITEPSRSRPDGLVVSPDGKLVAFNREVVY
                     ASGKFAQVFVADIELP"
     gene            2931..4211
                     /locus_tag="PLT86_11800"
     CDS             2931..4211
                     /locus_tag="PLT86_11800"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOT37337.1"
                     /translation="MTEAAESREAQRRPNGTEVRHMWDCGRPNPALRGAGFPVLDDVR
                     HSLIFAGERQAGAYNHHSKLTRHRGMFHAMWSNHPHGEDGPGQRVLYSHSSDGVRWSS
                     SVELFPPMQPPRPNGENGLVLTAWRWVKYEGRLFATAMCNENIGFENPDGSEIVPQRD
                     AGHRFLARRFFDGFCREIGENGPICPPILPMGDKVPPPAPAFVSLYSDDEEAMRLGRR
                     VREEMCRPENTPSWNPPGISLPRGVDTLRLCEPTVYRARCGEYVMLLRDGEFSHRLYA
                     SFSDDGRRWSSAVPTDIPDSPSLSTTVALPNGSVLLVGNMVASDFDNAAEVRHYLRDP
                     LVIAVSSDGSMFERAYALRCGTQQWRTPREEVRGRNGGAQYPSCVVENGILHVQYSMG
                     KEDIWMSSVALACLGPDGGGSLNERGTGVRSQGP"
     gene            4208..5935
                     /locus_tag="PLT86_11805"
     CDS             4208..5935
                     /locus_tag="PLT86_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT37338.1"
                     /translation="MNAEVLVEAEHFDDLGGWTLDTGFVESMGSPYLIAHGLGTPVAD
                     ATTTVRLPEPGTWRVWVRTFDWVARWDASGCPGRFEVLVDGEPVGVVFGTEGADWHWQ
                     DGGVIQIGKPDIRVSLHDLTGFNGRCDAICFSRDCTSPQPPLGCRPSVQAGREPGENA
                     SSRYDFVVVGGGYAGMCAAVSAARLGLSVALVQNRPVLGGNGSSEIRVPLRGLNPDET
                     PYPLLGRTLKSLEYDAQPNASKASDTDDAAIERILRSEPNLTLLLNHYVASVEMEGNR
                     IRAANAVCTRRPSSVRLEAHCFADCTGHGTLGALAGAEYAIAPRDLMGMTNIWSWEIA
                     PEPQDFPNTPWALDLKPGDFPVPPEGKSNGTWCWESGFNLDPIRDLEAIRDWNLRAVF
                     GAWSALKHGEEAQKYRNARLTFVSALGGVRESRRLIGDFVLSSDDMLQRVEFEDGFVP
                     VTWFLDRHVPDPNYSGPYRDNPFIATGIHEPGTDREARPRHGKPWWGIPYRCLYSRNI
                     VNLFMAGRNISTTYWALGAVRVMRTCGLMGEVVGTAAAVCARHGCLPREVYKTYLGEL
                     KRLLGASST"
BASE COUNT         1156 a         1775 c         1975 g         1088 t
ORIGIN      
        1 ctcgggaacg gagtgatcag gacgccggcc atagaccggc tcgtgcggga aggcacgtca
       61 ttccagcggg cgtacacacc ctcacctgtc tgtgtttcgg cgcggtgttc catgatttac
      121 gggcaatacc cgctccacac cggttgctac gacaacgcgc ccatgccgca ggacggccgt
      181 acctcattca tgggcgcgct gacggaggcg ggttaccgca cccacgggat cgggaaatgc
      241 catttctacc ccgatcgcgc gagcctgcga ggattccaga cgcgggaaag ccaggaagag
      301 ggtgtggctt cgcccgagca ggacgactac ctcgtctggc tgcgggcgaa cggttatgag
      361 catcttacgg accctcacgg tgtccgcggg gaaatgtatt acacaccaca accggcgcag
      421 atgccggcgc ggctgcaccc gacgcagtgg attggcgaca gagccacggc gttcctctcc
      481 gagccggaac gaaagagaga accgtggtac ctctacgcaa gcttcattca cccgcacccg
      541 ccgtttgccc cgccggtgcc gtggcacaag ctctaccgcg cgccactcat gccgttgccg
      601 aacgttccgg ctgagtacga agcgttgcac atctacataa accgctatca gaacaggtac
      661 aaataccgcg accagggcat tgaccagaac cttctgcggg cgatcaaggc ctattactac
      721 ggcgccgtgt cgttcatcga ttaccaggtg gggcggattc tggccgcgct tgaagaaacg
      781 gggcaactgg aaaacacgct gattgtgttc gcatcggacc acggcgagta tctgggagat
      841 tactactgtt tcgggaagcg tggcatgcat gacgccgccg cgcgggttcc cctgatcctg
      901 cgctacccgg aacgcttcgc cgccggtgcg gtgtgccaga agccggccag cctcgtggac
      961 atcgcgccga ccgttctggc ggccgcgggt gcccgcgtaa cagatcacga actcgacggc
     1021 gttgaccttt cggaactggc gtcaggggcc tgtccgcgcg agatggtgtt cagccagttt
     1081 cagaaggccg aacagggcgt ctacatggcg gtggacgagc ggtggaaata cttctacagc
     1141 gctcccgatg ggcgagagtt tctattcgac catcgggttg atccgctcga aacgcggaac
     1201 cgagtggcga cgccgttcat gaaggatcac ggggcgagga tgcgcgcggc gctgattagt
     1261 cacctgaagg cgggcgggga gaccgccgcg ctcgatggcg acggctggaa gcagtatccg
     1321 aagcgggaaa tgccggaaga cccggacgca gggttgttga ttcaggacag cccgtgggga
     1381 gtgttgaaga ttccggggta ttcggactga actacgatgg cgaagctggc tcggcaccga
     1441 cggggcgctc tcgcggagga aaccatgttg aaaaggtcat tcgcccggcg atgggtttcg
     1501 accacactct tcattctcgc aacgacggca ggttccatgg cggctgaacg gcagatcacg
     1561 aacgattcga cgcagaactg cgcgctggat cagaatcaca atttctcacc tgatgggaaa
     1621 tggctggtgt acgaccttcg cccgaacggc gggctgcaaa actgccggtc gatcgagaag
     1681 gtgaatgtag tgacgggaga acgcgtccgg gtgtacgatg gccctgattt cgtggagaac
     1741 atggggccgg gcatcgccgc gccaagttac ctccccgaac ggaacgaggt gattttcatc
     1801 cacggaccgt tcacgacgac cggccttgca tacgacatgg cgaggcggac gggcgcgatc
     1861 gttccggatg acggaagcgc aatgaacgac acatcccgca tcacgtgggc ggatgcccgg
     1921 gacgtaaccc ctccgttcac acccggcgcg cttcgaggtg gcacccaccg gcacgacccg
     1981 ggaggccccg gcggcctctg gatcgggtat acatacaacg atcaaatcat gaaacggtac
     2041 ggccagcaga tcggccggga cctcgatttg cgcatgctgg ggttgacgaa actgggtatc
     2101 ccggtatcgg tggatgcgga ccctgcgcat gagaaccgga gcggcgcggg gttctcggtc
     2161 gtcatcacgg aggtctttcc gaaggatgaa ctcgaccggg agccgggaac cgatcgaatc
     2221 taccgcgcga ccgacgacca gtgggttggc acgcgagggt accggaaacc ggacgggagc
     2281 tggcaactgg cgcgggcgtt tatcgggcac atgcgggtgc gtgcggccga cgggtctgtt
     2341 cgagaccacc gcgaagtgtt catcgtggac attcccgaag acatcacccg gcccggtccc
     2401 gcagggccgc ttgagggaac cggcaccacg tttcccgccc cgccggcggg cacttcccag
     2461 cggcggctga cccatacgga ttcgggatgc ttcggccttg tgcgttccac ctcagacggc
     2521 gggatgatcg cattcctttc ccgcgcggct gacggaacgc cgcaggtgtt tgtcgtttca
     2581 cctctgggag gagagccccg gcagataacg agcttccccg gaggagccag ccaccccgta
     2641 cggtggcttc ccgacgacct gcactttgtt acggtgagcg agggtcgtgt gtatgttgtt
     2701 gacgcagcca gcgggtgtgc gcgggcgatc actgagccct cccgcagccg cccggacggg
     2761 ttggtcgtat ccccggacgg gaagctcgtg gcgttcaacc gggaggtcgt ctacgcaagc
     2821 ggcaagttcg cgcaggtgtt cgttgcggat attgagttgc cgtagataga cgccttcctc
     2881 aaccagaccc gcgaaaggaa gctggcgcat ccgcagcttt ggaacccgcc atgacggaag
     2941 cagcagaatc gagagaagcg cagcggaggc cgaacggaac ggaagttcgg cacatgtggg
     3001 attgcggccg cccgaacccc gcgcttcgcg gcgcagggtt tcccgtgctc gatgatgtca
     3061 ggcattccct tatcttcgcc ggcgaacggc aggcaggcgc gtataaccat cacagcaagc
     3121 tcacgcggca ccggggcatg ttccacgcga tgtggagcaa ccacccacac ggggaagacg
     3181 gaccgggaca acgagtgctt tactcccact cgtcggacgg agtgcgctgg agcagttccg
     3241 tcgagttgtt cccaccgatg cagcccccgc ggccaaacgg ggaaaatggg ctcgttctga
     3301 cggcgtggcg ctgggtgaag tacgaaggcc gcctgtttgc cactgcgatg tgcaacgaaa
     3361 acatcggttt tgagaacccg gacggcagcg agatagtacc gcagcgcgac gccgggcatc
     3421 gattcctcgc aaggaggttc ttcgacggtt tctgccgcga aatcggggaa aacggtccga
     3481 tttgcccgcc catccttccg atgggagaca aggtgcctcc gcccgctccg gcttttgtgt
     3541 ctctgtattc ggacgacgag gaagcaatgc ggctcggacg tcgggtccgt gaggaaatgt
     3601 gccgccctga gaacacgccg tcatggaacc ctcccggaat cagcctgccc cgtggcgtag
     3661 ataccctccg actctgcgaa cccaccgtgt accgcgccag atgcggggaa tacgtgatgt
     3721 tgctgcgcga tggcgaattc tcgcaccgcc tgtacgccag cttctccgat gacgggcgca
     3781 ggtggagctc ggccgttccg acggatattc ccgattcgcc atcgctgtcg accaccgtgg
     3841 ctttgccaaa cgggagtgtt ctgctggtgg gcaacatggt ggcgtccgat tttgacaatg
     3901 cggccgaggt ccggcattac ctgcgtgatc cgcttgtgat tgccgtaagc agtgacggca
     3961 gcatgttcga gcgcgcgtat gccctccggt gcggcacgca gcagtggcgc actccgcgcg
     4021 aagaagtacg tggccgaaac ggtggcgcgc agtacccctc ctgtgtggtc gaaaacggga
     4081 tcctccatgt ccagtactcg atgggcaagg aagatatctg gatgagttct gttgcgctgg
     4141 cctgcctcgg tcctgatgga ggaggttccc tgaacgagcg aggaacaggt gtgaggagcc
     4201 aagggccatg aacgccgaag tgctggtcga agcagaacat ttcgatgacc ttggtgggtg
     4261 gactctcgac accgggttcg tcgaatccat gggctcgccc tacctgatcg cacacgggct
     4321 gggaactcct gtggcggatg ctacgaccac ggtaaggctt cccgaaccgg gcacatggcg
     4381 cgtatgggtg cggacgttcg actgggttgc ccgctgggat gcttccggct gccccggccg
     4441 cttcgaagtt cttgttgacg gcgagcctgt cggggtggtg ttcggcacgg agggcgccga
     4501 ctggcactgg caggatggcg gggttatcca gatcgggaaa ccggatatca gagtatccct
     4561 ccacgacctg accgggttca acggccggtg tgatgccatc tgcttcagtc gtgactgcac
     4621 gtccccccag ccgccgctcg ggtgccgtcc atcggtgcaa gcggggcggg aacccggcga
     4681 gaatgcctca agccgctacg atttcgtggt ggtgggaggg gggtacgcgg gcatgtgcgc
     4741 ggcggtttcg gctgcgcggc tcggcctttc cgtcgcactg gttcagaacc ggccggtact
     4801 cggaggaaac ggttcgagcg agattcgcgt gccgttgcgc ggactgaacc ccgacgaaac
     4861 cccgtatccg ctcctcgggc ggacgctgaa atccctggaa tatgacgcgc aaccgaatgc
     4921 ctccaaggcg agtgatacgg atgatgcggc aatcgaacgc attcttcgct cagaaccgaa
     4981 ccttacgctt cttctgaacc attacgttgc ctcggttgag atggagggga atcgcatccg
     5041 cgccgcgaac gcggtgtgta cccggcgacc ctcttccgtg cgactggaag cgcactgctt
     5101 tgccgactgc accggacacg gcacgctggg ggccctcgcc ggcgcggaat acgccattgc
     5161 cccgcgcgac ctcatgggaa tgacgaatat ctggtcgtgg gaaatcgccc ctgaacccca
     5221 ggatttcccc aatactccgt gggcgctgga tctgaaaccg ggcgatttcc cggttcctcc
     5281 ggagggcaaa tcgaacggga catggtgctg ggagagtggc ttcaatctgg acccaatccg
     5341 cgacctggaa gccattcggg actggaacct gcgggcagtg ttcggcgcgt ggagcgcact
     5401 caagcacgga gaagaggcac agaagtacag aaatgcgagg ctcacgtttg tatcggccct
     5461 gggcggcgtg cgcgaatcac gaaggctcat cggagatttc gtgctctcgt cagatgacat
     5521 gctccagcgg gtggagttcg aggacggatt cgtgccggtg acgtggtttc tcgatcgcca
     5581 tgtaccggac ccgaactact cgggaccgta ccgggataat cccttcattg cgacgggcat
     5641 ccacgagccg ggaacagaca gagaagcgcg cccacgacat ggaaagccct ggtggggcat
     5701 tccgtaccgc tgcctgtaca gccgcaacat cgtcaacctt ttcatggccg gccggaacat
     5761 cagcactacg tactgggcgc tcggggcggt cagggtaatg cgcacgtgcg gtctgatggg
     5821 tgaagttgtc ggtaccgcgg cggcggtgtg cgcccgccat ggttgtcttc cgcgtgaggt
     5881 gtacaaaacg tatcttggag agttgaaacg acttctgggt gccagttcca cataatgttg
     5941 aggcaggcac cgtgtgacga accaggaatg atacgggtct gcgggggcgg tccg
//
LOCUS       DAOSOO010000195         5836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13838_length_5836_cov_6.319841, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000195 DAOSOO010000000
VERSION     DAOSOO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13838_length_5836_cov_6.319841"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..908)
                     /locus_tag="PLT86_11810"
     CDS             complement(213..908)
                     /locus_tag="PLT86_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT37339.1"
                     /translation="MPITILLVEDEPGIRSVTAKWLAHHGYAVDEAENGKEGWECIQQ
                     RAPDLVITDIAMPEMDGVELCRLIKTTESTADIPVLMFTSHAEADLQLAGTKAGADAY
                     IPKNGDLRVLNARIEALAAARSRARQETERTRRLTLSQSVTTLAHHINNSVIAIHATA
                     SVVDPNNPQQAQKLRHVCQTEARKMLLVLKALKEMAEREELKTTVYVGNELMFDLEAA
                     LRQLDSGAKSPPR"
     gene            complement(918..>2176)
                     /gene="amrA"
                     /locus_tag="PLT86_11815"
     CDS             complement(918..>2176)
                     /gene="amrA"
                     /locus_tag="PLT86_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOT37340.1"
                     /translation="GRIAAIAYRQVQGREYDAVVILAPSHREAFAGVSLMPAGGYETP
                     LGIAGIHEGIARKLLEQGGCIHETRKGHGPEHAVEVQIPFIQRALGSVPIVPLVMGEH
                     SWPVCNSVARAIARAVEGMNVLVVASSDLYHGDSEAECETIDRVTLAAAESRPPQQFC
                     ADLDAGRAQACGGAPIAVAKEYARLLGASGVRVLAHATSADVTGHRGGYVVGYGAIAY
                     FRNGAAVEQSSLSEEDRALLLRLARESVRAAAAGRPLPQPPKGHPALEEPKGAFVTLH
                     KDGDLRGCIGLLQAYQPLAATVIEMAQAAAINDPRFPPLREHELDTTTLEISILSPFE
                     VTKPEQVEVGTHGIFITGRGRRGVLLPQVATEQGWDRETFLEHVCLKAGLPRNAWQSS
                     DVVLERFSAEIIHDTGPIGREETRRN"
     assembly_gap    2177..2276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2277..2503)
                     /gene="amrB"
                     /locus_tag="PLT86_11820"
     CDS             complement(<2277..2503)
                     /gene="amrB"
                     /locus_tag="PLT86_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT37341.1"
                     /translation="MNTQAPIRTAAVAGQFYPESPDVLREEVDALLAAAPKDDRITGD
                     IVAIVAPHAGYIYSGRIAAIAYRQVQGREYDA"
     gene            2816..3763
                     /locus_tag="PLT86_11825"
     CDS             2816..3763
                     /locus_tag="PLT86_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT37342.1"
                     /translation="MSVLYAPWGVVYSPGYALDMSGQESVHPFDVRKYMKIVDLLHAD
                     GLLDGGAIIEPDLLTEQEAALVHTPDYMRLWTDPETMAEIFEVDYLRNVSPDTIRERY
                     LPPYFAMAGGTVRAARDALKCGTVFNIGGGFHHAHADHGSGFCFVADVAIAIRVLMRE
                     KLLQRAAIVDCDVHQGDGNATIFAGDPAVFTLSFHQEDIFPFEKATSSRDVPFPAGTG
                     DEAYMALLREHVPDVLDTHRPDIVFFVAGSDVHADDPLAATELTSAGIIQRDAFVWSE
                     TRKRGIPFVMVLAGGYFPRCPEVHAASIGNIIRKARKQV"
     gene            3760..4776
                     /locus_tag="PLT86_11830"
     CDS             3760..4776
                     /locus_tag="PLT86_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37343.1"
                     /translation="MRRRGFAEEVLQTHSGETKMDWATIKGFNYQPSWGSSGFEIWRN
                     FDAERMGHELALGRLYFPRMNAIRLWLSHDAWTRSPEKFETAFSAALRIAEKHSLRVM
                     PVLFNRWHDATLDYGGVYLDHFLPKASWVQARDRTAEFAEAVFTAHAADSRVFAWDLC
                     NEPFSYGCAPAEIPEIVKAEFAWLEKTYRIGTRCGVKAPITVGIHPGHGIEGIRQIEP
                     ISDILSIHPYWTLDSPPHDKGAYEKLLDDYVRFAERAGKPLLATETCWGATDDAKRVE
                     IIRYTLGELKKRRIGWLVYVLHHSLIADAHRIEFGPLSTPGNLAFIEADGTLRPGHEV
                     FNEF"
     gene            5235..>5836
                     /locus_tag="PLT86_11835"
     CDS             5235..>5836
                     /locus_tag="PLT86_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT37344.1"
                     /translation="MKVYDSVFDGHAALVLERSRRVHEEIAEGLTKEGAIPLTVGAFQ
                     MRNRCGGETGKRQNLERMLSAIRTAAAQGVQMLAFPEMCLPGYFTPVSGTAAEATAAN
                     HALADIVGESPYVEALKAAARDARMVLAFGFAEKADGVIYNAVGVIDADGTWLGTRRK
                     APLYPWPYELDSFAEPGPEHRCVVFRTNYATVGISNCFDG"
BASE COUNT         1059 a         1711 c         1877 g         1089 t
ORIGIN      
        1 cgccgctcgg acttcgtcct cccgccactc ggagaccgtc ctcggcttaa ccttccaaaa
       61 aaatcctgat gcaaatctgc aacttcttcg cgaaaagcct tgacacgaag acacctcctt
      121 gcctcagtaa gaaagaggga cggagtggga gagcggacta tacgtcctac acgacctatt
      181 acagcgcaag cggggggaag ggagacctcc ggtcagcgcg gcggggattt ggccccggaa
      241 tcaagctgcc tgagggcggc ttcgaggtcg aacatcagtt cgttgccgac gtagacggtg
      301 gttttcagct cctcgcgttc ggccatctct ttcagcgctt tcagcacgag gagcatcttc
      361 cgcgcttccg tctggcagac gtgtcgaagc ttttgagcct gttgcgggtt gttcggatca
      421 accacggagg cagtggcgtg gatggcgatc acgctgttgt tgatgtggtg cgcgagggtg
      481 gtaacgctct ggctcagggt gagccggcgc gtccgctcgg tttcctgccg ggcgcgggac
      541 cgcgcggccg cgagcgcttc aatgcgcgcg ttcaacacac ggaggtcgcc atttttggga
      601 atgtatgcgt ccgcacccgc tttggtgccg gcaagctgga ggtccgcttc cgcatggctc
      661 gtgaacatga gcaccgggat atccgcagtg gattccgtcg ttttgatcaa ccggcagagc
      721 tccacgccgt ccatctccgg catggcgatg tcggtgatga cgaggtcggg ggcgcgttgc
      781 tgaatacact cccacccctc tttgccgttt tccgcctcgt cgaccgcgta cccgtggtgg
      841 gcgagccact tcgccgtcac gctgcggatc ccgggctcgt cttccacgag cagaatggtg
      901 atcggcatcg cagacaccta attccgccgg gtttcttcgc ggccgatggg tcccgtgtcg
      961 tgaatgatct ccgcggagaa gcgctccagc accacgtcgc tgctctgcca cgcgttgcgc
     1021 gggagccccg ctttcaggca cacgtgttcg aggaaggttt cgcggtccca cccctgttcg
     1081 gtggccacct gcggcagcag aacgccgcgc cgtccgcgac cggtaatgaa tatgccgtgt
     1141 gtgccgacct cgacctgttc cggcttcgtt acctcaaatg gcgaaagaat ggaaatttcc
     1201 agcgtcgtcg tgtcgagttc atgctcccgc agagggggaa atcgcgggtc gttgattgcc
     1261 gcggcctggg ccatttcgat gacagtggcg gcgaggggct gatatgcctg cagcagtccg
     1321 atgcaaccgc gaaggtcgcc gtctttgtgc agcgtaacga aggcgccttt tggttcttcc
     1381 agtgcggggt gacctttcgg cggctgaggc aacggccggc cggcagccgc cgcgcgaacc
     1441 gattcacgtg caagtctcag cagcagagcg cggtcttcct cgctgaggga ggactgctcc
     1501 accgcggccc cgtttcggaa gtacgcaatt gcgccatatc cgactacgta gccgccgcgg
     1561 tggccggtga cgtcggcgga cgtggcatgc gccagcacgc ggacaccgga agcaccaagg
     1621 aggcgggcgt actccttcgc caccgcgatc ggggcgccac cacaggcctg cgcccgcccc
     1681 gcatccagat cggcgcagaa ctgctgcgga ggacgggatt ccgcggctgc cagtgtcacg
     1741 cggtcaatgg tttcgcactc cgcttccgag tcgccgtggt agaggtcgct ggacgccacg
     1801 acaagcacgt tcatgccctc gactgctctg gcgatcgcac gtgccacgga gttgcatacc
     1861 ggccacgaat gctcgcccat caccaggggg acgatcggca cacttcccaa cgcgcgctgg
     1921 atgaacggaa tctgcacttc caccgcatgc tcggggccgt gtcctttccg tgtttcgtgg
     1981 atgcagccgc cctgctccag cagttttcgg gcgatgccct catgaatccc cgcgatcccg
     2041 aggggggtct cgtatccccc cgccggcatg agggagacgc cggcaaatgc ttcacggtgg
     2101 gacggtgcca gaatcacgac cgcgtcgtat tctctgccct gaacctgccg gtacgcgatc
     2161 gcggcgatcc gcccgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgt
     2281 cgtattctct gccctgaacc tgccggtacg cgatcgcggc gatccgcccg ctgtagatgt
     2341 agccggcgtg cggcgccaca atcgccacga tgtcgccggt gatgcggtca tcttttggcg
     2401 ctgccgccag aagggcgtca acctcttcgc gaagaacatc cggggattcc gggtagaact
     2461 gaccggctac ggcagcggtt cgtatcgggg cttgtgtatt catcggaccc tccatgttcg
     2521 gcacgaacgc aaggaggagc gccgcaatgc ggaagagaac ccgctcacag agcgaacgtt
     2581 tctggtgaaa gatgcgtagc ggcattgcct taccgtgaaa agtgtgaaac agggcgcgac
     2641 gggtcaatgt tcgtacgaga aaacggggct tcgtcgtatc tttgaacaag gcctccggac
     2701 cggcgccaca ggcggaaact gatgatggag gggagttttc gcgagggaac gcctccgcaa
     2761 acccgcgccg cttccgcgga tggtacggtg aatcgttttt gatcgggatt gaatgatgag
     2821 tgttttgtac gctccatggg gcgtcgtgta cagccccgga tacgccctcg acatgtcggg
     2881 gcaggaatcg gtccatccgt ttgacgtgcg caagtacatg aaaatagtcg atctgctgca
     2941 tgccgacggg ctgctggacg gcggggcgat catcgagccg gacctcctga cggaacagga
     3001 ggccgctctg gtgcataccc ccgactacat gcgtctctgg accgatcccg agaccatggc
     3061 ggagattttc gaggttgatt acctgcggaa tgtatcgccc gacacgatcc gggaacgcta
     3121 tctcccgccc tatttcgcaa tggcgggcgg cacggttcgg gcggcgcggg atgcgctgaa
     3181 gtgcgggact gtgttcaaca tcggcggcgg gttccaccac gcccacgccg accacgggtc
     3241 cggattctgc ttcgtggcgg atgtggcgat tgccatccga gttctcatgc gcgaaaagct
     3301 gctacaacgc gcggcgattg tggattgcga cgtccaccag ggcgacggca acgcgaccat
     3361 ttttgccgga gatcccgccg tattcacgct ctcgtttcac caggaggaca tattcccgtt
     3421 tgaaaaggcg accagttcgc gcgatgttcc gttcccggcg ggaacaggtg acgaggccta
     3481 catggcgctt ttacgggagc atgtgccgga cgtgctggat acccacaggc cggacatcgt
     3541 tttctttgtc gccgggagcg acgtgcacgc cgatgatccg ctcgccgcga cggagctgac
     3601 gagcgcgggg atcatccaga gagacgcgtt cgtgtggagc gaaacgcgga aacgtggcat
     3661 tccctttgtc atggtgctgg cgggagggta cttcccccgg tgccccgagg tgcatgccgc
     3721 gtccatcggg aatatcatca ggaaggcgcg gaagcaggtg tgaggcggcg gggcttcgcc
     3781 gaagaagtgc tgcagaccca ttcgggggag acaaagatgg actgggcgac aatcaaaggc
     3841 ttcaattacc agccaagctg gggcagttcg gggtttgaaa tctggcgaaa cttcgacgcg
     3901 gagcgcatgg ggcacgaact ggcgctggga aggttgtatt tcccacgcat gaacgctatc
     3961 aggctgtggc tttcgcacga cgcatggacg cgcagcccgg agaagttcga aacggcgttc
     4021 agcgcggcat tgaggattgc ggaaaaacac tcccttcgcg tgatgccggt cctgttcaac
     4081 cgatggcacg acgccacgct cgattacgga ggggtctatc tcgaccactt cctgccaaag
     4141 gcaagctggg ttcaggcgcg ggatcgcacg gcggagtttg cggaagccgt tttcaccgct
     4201 cacgccgccg acagccgcgt cttcgcgtgg gatctctgca acgaaccgtt ttcctacgga
     4261 tgcgcgccgg cggagatacc tgaaatcgtc aaggccgagt ttgcgtggct ggagaagacg
     4321 taccggatcg gcacgcggtg cggcgtcaag gctccgatca ccgtcggaat tcatccggga
     4381 cacgggatag aaggcattcg tcagattgaa cccatcagcg acatcctgag cattcacccg
     4441 tactggaccc tcgacagccc cccgcacgac aagggcgcgt atgagaaact gctggacgac
     4501 tatgtccggt ttgccgaacg cgcgggaaag ccgctccttg caacggaaac gtgctggggc
     4561 gcgacggacg atgcaaagcg ggtcgaaatc atccgttaca cgttgggaga gctgaagaag
     4621 cggcgcatcg gatggctggt ctacgtgctt caccacagcc tcatcgccga cgcgcaccgg
     4681 atcgagttcg ggccgctcag cacccccggc aaccttgcgt tcattgaggc agatggaacg
     4741 ctgcgcccgg gacatgaggt gttcaacgag ttctgagcgt ggatcagagc ggggtcgtct
     4801 cgatacgtcg agcgcgtctt gcgcccgcca ctcgacgacc gccgccgctg gcagacggaa
     4861 acgagactcc cgcctgcgcg ggaatgacga tttggctatg cgttggggtc tgtcgggccg
     4921 gcaggaatga cggcctgggt gcaccgggag actggaacga accgggaacg cagggctcgt
     4981 gggtggctga gcctttggaa atgacgaatg tctgatctgc cgcagggatg attctgggtt
     5041 cccgccgcgc tttgcgctct aagggaaccc gggaatgcct tgccggcaat tgatgcgcaa
     5101 cacgaaatgc cgaaattcgc ctttgtgcat gaggcaaact gcacgcccct tcggcaactg
     5161 ctgtcattcc cgcgaacgcg ggaatccagc ccctcaggcg tgacggggaa aggcggcttt
     5221 tcgcgggcgg tgagatgaag gtctacgact cggttttcga cggacatgcg gcgctggtgc
     5281 tcgaacggag caggcgcgtg cacgaggaga tcgcggaagg gttgaccaag gagggcgcca
     5341 tcccgctcac cgtcggtgcg tttcagatga ggaatcggtg cggcggggaa accggaaaac
     5401 ggcagaatct cgaacggatg ctttcggcca ttcgaacggc ggcggcgcag ggcgtccaga
     5461 tgcttgcctt ccccgaaatg tgcctgccgg gctacttcac gccggtgtcg ggaacggcgg
     5521 cggaggcaac cgccgcgaac catgcgctcg ctgacattgt gggagagagc ccttatgtgg
     5581 aggcattgaa ggccgcggcg cgggatgccc gcatggttct ggcgttcggg ttcgcggaaa
     5641 aggccgacgg tgtcatctac aacgccgtcg gtgtcattga tgccgatgga acatggcttg
     5701 gcacgcgccg gaaggcacct ctgtatccgt ggccgtacga actggacagc ttcgccgagc
     5761 cgggcccgga gcatcggtgc gtcgtgtttc gcacgaacta cgccacggtc ggcatctcta
     5821 actgcttcga cggcga
//
LOCUS       DAOSOO010000196         5801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13957_length_5801_cov_5.234076, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000196 DAOSOO010000000
VERSION     DAOSOO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13957_length_5801_cov_5.234076"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..3198)
                     /locus_tag="PLT86_11840"
     CDS             complement(49..3198)
                     /locus_tag="PLT86_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOT37345.1"
                     /translation="MTNAQVKADIAANLPDNTSGLITPAKLRTEMCNMVDYSDTAAAA
                     ITLAGLGGVPTTRTVNGHALSSDVTVTASDIGLGNVTNAAQVLRTEMGAANGVATLES
                     GGKLPLSQIPDALIGQVKYQGSWNASTNTPTLADPPASTTKGYYYVTSTAGTQFGISF
                     EVGDWIISDGSMWQKVDNTDAVVSVAGRIGAVTLTTADLADFSNAVLAASPVTSVAGR
                     TGVITLTAGDVGLGSVENTALSTWAGSANLATVGTIASGTWNGTAIADSYISSAAAWN
                     AKVSFPGFGTTAGTAAEGNDVRLSDARTPLAHNQAWSTITSTPTTLSGYGITDAQGLD
                     SDLTALAGLSSNGLIARTADGAAATRTITGTTNQVIVSNGDGVAGNPTLSLPQSIATT
                     SNVQFGNLQLGSSESSPATYGGQLKAYSADNTSLESVGGIELPVTTNGYGVKIQALSA
                     DGAALAIANRNGAATWTERMRISADGKVGIGTTAPAVTLDVKVSPNWHFGVFDFTLGA
                     ASIGAFNDGFSGWQPLSINPGGEVWFLGGNVGIGTSSPGSLLHLSAENPTLSLVPYTG
                     GFGRTSSIDLFGTFGYASNDDTRARRLAQIKVGSENWGAWESAYMAFVGITPDDGSPA
                     ELVHINTYNGAVGIGTSSPAARLDLGASTGVVQYIYNDDSANAGLGINLSGGSCELSL
                     FAGADGDDAGRISFGRRRVDTGTYTERMVVDATGNIGIGTSSPAAQLHLRSGSDNIAE
                     AIRLDNPTDESDNGSKIVWRNADVSKDAAFFAARRIGSSNGMALTFGTAEDWSTAAAT
                     EKMRIAGNGNVGINNTNPQRKLDVVGDDSGNGIRVTGTTGTIGAFTAEGTSVNIQADS
                     ADATRVLVDASANGSGFVELNGGSSGYVQLGTGGTAQMQVFANGNVKLNSYGAGTLQT
                     DSSGNVTASSDARLKNVTGDFSRGLADVLKLTPRTYHWNEKSGMNTEDENVGFIAQEV
                     QDAIPEAVGTMKTTDYEEDDAATGKKVRKSKREAAEYLTLSDRPLIAALVNAVKELKA
                     ENDTLRARVSALENGKAAK"
     gene            complement(3211..3645)
                     /locus_tag="PLT86_11845"
     CDS             complement(3211..3645)
                     /locus_tag="PLT86_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37346.1"
                     /translation="MLTKSSLWATATPTAAKLPISDGDGKLAVGWVRYNYAPTVTVAV
                     DTLLDDTHAVVLVSAAATISLPTAVGVAGRLYNVVRTGDGEVTIQPDGTETISGDTPL
                     VLTSQWDSVVFVSDGTNWVKLGMEKKSSISVAFSSGFSSGFQ"
     gene            complement(3714..4895)
                     /locus_tag="PLT86_11850"
     CDS             complement(3714..4895)
                     /locus_tag="PLT86_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37347.1"
                     /translation="MALDLANLQNQFLQSKNLFQDPIYQWVWRTNPFISLFDRKEFNE
                     QDGLVPEVVTTTGELPTAYPDLDNLTVSNGSGSAACDIEASEVRFGNIRRTYQLEVDA
                     ARSPIFCLTDLQFDWQAAQMARNAQQMLGQYATVRWADWYRMKNISMINTKVATLANG
                     AIDTGEDSTTGFTSVDTPTTALSWDHLNPLYDEMLRNGGELHAPGMSEGMPVFTLAVG
                     PGMKRALFQTDTKVRDTVNWIDAGNRVAQNFQALGINTSINGFAPLVDKFPVRFAADG
                     TTKIYPTINVAASGGWKHAPNPAYKTVANGGDAVYEVVSVLCRDIYEVRPRPTGTTQY
                     GLQGFNPINYVGDVQWINNPTFGGVNDQGNKGYYRMDFQMAAKPVRPEIGYSILTLAI
                     D"
     gene            complement(5152..>5801)
                     /locus_tag="PLT86_11855"
     CDS             complement(5152..>5801)
                     /locus_tag="PLT86_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37348.1"
                     /translation="VLDYNEIPANLESLMGKPLKEFSAEVSKLTKDMNSMDATAVQTS
                     LRNAWKIHNEATAALSKSQELAKALSEKSAHAQKQAVEKVWSTFKEAESILAKRPVPE
                     GATPEELAEITSYNEAVQGLRSNYERNAFGRMTEEETARMALKATTFDFIQTHGVKMM
                     ERAYKSMEARALAAEKQLNELKAARSPGNMGGDPQKATASKPMDFDTLFSQRPQR"
BASE COUNT         1032 a         1694 c         1860 g         1215 t
ORIGIN      
        1 cggcgagcgc gatcagcagc gcgtgggggc gggtcatggg acggtgtttc acttcgcggc
       61 ctttccgttt tcgagtgcgg atacgcgggc gcggagggtg tcgttctccg ccttcagctc
      121 cttcactgcg ttgacgagcg cggcgatgag cgggcggtcg gagagtgtga gatactcggc
      181 ggcctcgcgc ttggacttgc gtaccttctt cccggtcgcg gcgtcatcct cctcgtaatc
      241 ggtcgtcttc atcgtgccaa ccgcttcagg gatcgcgtcc tgcacctcct gcgcgatgaa
      301 gcccacgttc tcgtcctcag tgttcatgcc ggacttttcg ttccagtggt aggtgcgagg
      361 cgtgagcttg agcacgtccg caaggccacg ggagaaatcc ccggtcacgt tcttgaggcg
      421 ggcgtcggag gaggcggtga cgtttccgga gctgtcggtt tggagcgttc cggctccgta
      481 gctgttcagc ttcacgttgc cgttcgcgaa cacttgcatc tgtgcggtgc cgccggttcc
      541 caactgaacg taaccactgg acccgccatt cagttcgacg aagccagagc cgtttgctga
      601 cgcgtccaca agaacacggg ttgcatcggc tgaatcggct tggatgttca cggacgttcc
      661 ttccgccgta aatgcaccga ttgttcccgt tgtgccagtg acacgtattc cgtttccgct
      721 gtcgtcacca acaacgtcca acttgcgttg aggattcgtg ttgttgatac cgacgtttcc
      781 gttaccggcg atgcgcattt tttccgtagc ggccgccgtt gaccaatctt ccgcggtgcc
      841 aaacgtaagg gccattccgt tgctggaacc gatacgtcgg gcagcaaaga acgcggcatc
      901 tttgctaaca tcagcgttcc gccacacgat cttgctgccg ttgtcggact cgtcggtcgg
      961 attatctaga cggatagctt cggctatatt gtctgacccc gaacggaggt gcagttgtgc
     1021 ggcgggcgaa gacgtaccga taccgatatt gccggtcgca tcaacgacca tccgctccgt
     1081 gtaggttccc gtatccacac gccgacggcc aaaagagatt cttcctgcgt cgtctccgtc
     1141 cgcgccggcg aacagtgaaa gttcgcagga acctcccgat agattgatgc caagtccagc
     1201 gttcgcggaa tcatcgttat atatgtactg aacgacgccg gttgatgctc caagatcaag
     1261 acgtgcggcg ggcgaagacg tgcctatgcc aaccgcgcca ttgtaggtgt tgatatggac
     1321 aagttccgct gggcttccgt catcaggggt aatgcccacg aatgccatgt acgcgctttc
     1381 ccatgcgccc caattctccg aaccaacctt gatctgcgct aggcggcggg cgcgcgtgtc
     1441 atcattggac gcataaccga aagttccaaa cagatcgatt gaggaggtcc gcccaaaacc
     1501 accggtgtag gggaccaagc tcaaagtcgg attttctgcg gagagatgca gcaacgatcc
     1561 ggggctggaa gtgccgatgc cgacattgcc gccgagaaac cagacttcac caccgggatt
     1621 gatcgaaagc ggctgccagc ctgagaaccc atcgttgaac gcaccaatcg atgctgcacc
     1681 caaagtaaag tcgaatactc caaagtgcca gtttggggag accttcacat ccagagtgac
     1741 agctggcgcc gttgtcccga taccgacctt gccgtcggct gaaatccgca tccgttcggt
     1801 ccacgtagct gcgccgttgc ggttggcaat tgcaagggct gccccatccg ctgacaacgc
     1861 ctgaatcttc actccgtatc cgtttgttgt cactggcagt tcaataccgc cgacggactc
     1921 caagctagtg ttgtcggcag agtatgcttt caattgcccg ccgtaggtgg ctggggagga
     1981 ctctgatgaa cccaactgga gatttccgaa ctggacgttg cttgtggtgg cgatgctctg
     2041 agggagtgac agtgtggggt tgcccgccac tccgtcacca ttgctcacga ttacttggtt
     2101 ggtcgtcccc gtgattgtgc gcgtggcagc cgctccgtct gcggtgcggg cgatcaaccc
     2161 gttcgagctc aggccagcga gggcggttag gtcggaatcc agcccctgcg catcggtgat
     2221 cccgtaaccg gatagggttg tgggggttga agtgatagtg ctccacgcct gattgtgggc
     2281 gagaggagtc cgggcatcac taagccggac atcgttgccc tcggctgcgg tgcccgccgt
     2341 cgtgccgaag cccgggaacg aaaccttcgc gttccacgcc gcggcactgg aaatgtagct
     2401 gtcggcgatg gccgtgccgt tccacgttcc ggaggcgatg gtgccgacgg tcgcgaggtt
     2461 cgctgagccg gcccacgtgg agagcgcagt attctcgacg gagcccaggc cgacgtcccc
     2521 agcggtcaac gtgatcaccc cggtacgccc tgctaccgac gtgactggag acgcagcaag
     2581 caccgcgttt gagaagtcgg cgagatccgc agtcgtgagc gtgaccgccc cgattcgccc
     2641 agcgacactc acgacagcgt cggtattgtc gaccttctgc cacatcgagc cgtcgctgat
     2701 gatccaatcg ccgacctcga acgagatccc gaactgcgtg cccgctgtgc tggtaacgta
     2761 gtagtacccc ttggtcgtgg acgcgggcgg gtcagccagc gtcggggtat tagtcgaggc
     2821 gttccacgat ccctgatact tcacctgccc gatgagcgca tcggggatct gcgacagcgg
     2881 aagtttacca ccggactcca gggtagctac tccgtttgct gcccccattt cagtgcgcag
     2941 cacctgcgcg gcgttggtga cgttgcccag gccgatgtcg gatgccgtaa cagtcacgtc
     3001 acttgagagc gcgtgaccgt tgacggtgcg cgtcgtcggc acgccgccga ggcctgcgag
     3061 cgtgatggcg gcggctgcgg tgtcggagta gtccaccata ttgcacatct cggtgcgcag
     3121 cttggcgggg gtgatgagcc cggaggtgtt gtcgggcagg ttggcggcga tgtcggcttt
     3181 aacttgggcg ttggtcatgg gggcggaggg ttactgaaat ccggaactga aaccagagga
     3241 gaaggctacg gagatagagg acttcttctc catgccgagt ttgacccagt tcgttccgtc
     3301 ggagacgaat acaaccgagt cccattgcga agtcaaaaca agaggcgtgt ccccgctgat
     3361 tgtttcagtg ccatcgggtt gaatggtgac ctccccatcc cccgttcgca ctacgttgta
     3421 gagccggccg gcgacgccga cggcggtggg aagagaaatt gtcgccgccg cgctgacgag
     3481 cacgacggcg tgggtgtcat ccaggagggt gtccacggcg accgtgacgg tcggcgcgta
     3541 gttgtaccgc acccagccga cagcgagctt cccatcccca tcggagatcg gcagcttggc
     3601 ggcggtaggc gttgcggtcg cccacagact ggacttcgtg agcatttagg aaagggggcc
     3661 actacccgaa aggatagtgg cccccgcgag ttacctacta gccactacca aggtcagtcg
     3721 atggcgaggg tgaggatcga gtagccgatc tccggacgga cgggcttcgc agccatctgg
     3781 aagtccatac ggtagtaccc cttgttgccc tggtcgttga cgccgccgaa ggtggggttg
     3841 ttgatccact gcacgtcgcc gacgtagttg atcgggttga agccttgcag gccgtactgg
     3901 gtcgtgccgg tcgggcgcgg gcgcacctcg tagatgtcgc ggcacaggac cgagaccacc
     3961 tcgtacacgg catcgccgcc attggcgacg gtcttgtagg cggggttcgg ggcgtgcttc
     4021 caaccaccgg aggcggcgac gttgatcgtc gggtagatct tggtcgtgcc gtcggcggcg
     4081 aagcggaccg ggaacttgtc caccagcggc gcgaagccgt tgatcgaggt gttgatgccc
     4141 agcgcctgga agttctgcgc cacgcggttg ccggcgtcga tccagttgac ggtgtcgcgg
     4201 accttggtgt cggtctggaa cagcgcccgc ttcatgcccg ggccgacggc caaggtgaag
     4261 accggcatac cctcggacat gcccggagcg tggagttcgc caccgttgcg cagcatctcg
     4321 tcgtagaggg ggttgaggtg gtcccacgac agcgcggtgg tcggagtatc gacggaggtg
     4381 aagcccgtgg tcgagtcctc accagtgtcg atggcgccat tggccaaggt ggcaaccttg
     4441 gtgttgatca tcgagatgtt cttcatccgg taccagtcgg cccagcggac cgtggcgtac
     4501 tggccgagca tctgctgggc gttgcgggcc atctgcgcgg cctgccagtc gaactggagg
     4561 tcggtcaagc agaagatcgg cgagcgggcc gcatcaactt cgagctggta ggtgcggcgg
     4621 atgttgccga agcgcacctc ggacgcctcg atgtcgcagg cggcggaacc gctgccgttg
     4681 gacaccgtga ggttgtcgag gtcggggtag gcggtcggca actcgcccgt ggtggtgacc
     4741 acctcgggga cgagaccgtc ctgctcgttg aactccttgc ggtcaaacag ggagatgaag
     4801 gggttcgtac gccagaccca ctggtagatg gggtcttgga acagattctt cgactggagg
     4861 aactgattct ggagattggc gagatcgaga gccatgatga aaggagagtt aggacacgcc
     4921 gcgtcttcgg ctagtcagac ccgaatggcc tgctaccaac gcggtttttt ggcggtgccc
     4981 acgataggcg tggtgccaac aaccgtcccg ggggtgatct ccggctacac cttcagaatc
     5041 ccaaggtgag cggcctcggg tctattacgc tcgaccggca aaatatgcct actctccgat
     5101 tcgtcaaaca aaaagccccc ggctccgaag agccgagggc agtaggggta attaccgttg
     5161 agggcgttgg gagaacagcg tatcaaagtc catcggcttg ctagccgtcg ccttctgggg
     5221 gtctccaccc atgttccccg gagaacgcgc ggctttcagc tcgttgagct gcttctccgc
     5281 cgcaagggca cgggcctcca tgctcttgta cgcccgttcc atcatcttga cgccatgggt
     5341 ctggatgaag tcgaacgtgg tggccttgag ggccatcctt gcagtctcct cctcggtcat
     5401 gcgaccgaag gcgttgcgct cgtagttgga ccgcaggccc tgcaccgcct cgttgtagga
     5461 ggtgatctcc gccaactcct cgggagtcgc gccctcgggg acagggcgct tggccaggat
     5521 agactcggct tccttgaatg tgctccagac cttctctacc gcctgcttct gggcgtgggc
     5581 ggatttctcg gagagcgcct tcgcgagttc ctgcgacttg gagagggctg cggtggcttc
     5641 gttgtggatc ttccaggcgt tgcgcagact ggtctgaaca gcggtggcat ccatgctgtt
     5701 catgtctttg gtcaacttgg aaacttcggc gctgaactcc ttcagcggct tgcccatcag
     5761 cgattcgagg ttcgccggga tctcgttgta gtcgagcacc t
//
LOCUS       DAOSOO010000197         5792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_13991_length_5792_cov_5.186160, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000197 DAOSOO010000000
VERSION     DAOSOO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_5792_cov_5.186160"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1565)
                     /locus_tag="PLT86_11860"
     CDS             complement(339..1565)
                     /locus_tag="PLT86_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37349.1"
                     /translation="MRVMLSCVGNRDPFGSVDKRTGQNSEGPLLTALRIVEPDLLLLF
                     PSAGEEGTATTKIPDVMKRLEETGFPRDRVAIHPIAPENPADATDVLQAMGEVVPAAL
                     ADVPEDAELFVSISSGTPQMQQTWFVLSMLGRLRNASLWQVMDPTKLDPEDSPVWPVR
                     IDVLRLPAVLDEVEAAVTGCHFARAARALAGQGNRMQRGPQKSALLALAHVVEAWDLH
                     DRMDYDGCVELLETAGNECVQTPGLEPLSRLAAQQLQVVRAHGERTGLVFAYLMVERR
                     LARNDMIGALLLAVLVLELSVKAGLHPESPEESLWEHIKSAHRRQDPRWVAVEKAAGS
                     FSAVDSIRDHRNDVVHKLRSPDPVRVQRAAAVARAALRNLVSVFDDEIDDNPFNPAAQ
                     KSAIREVLDLLRKEPV"
     gene            complement(1562..2746)
                     /gene="csm5"
                     /locus_tag="PLT86_11865"
     CDS             complement(1562..2746)
                     /gene="csm5"
                     /locus_tag="PLT86_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOT37350.1"
                     /translation="MKQYTLTIEPVTPVHIGTGEKVGPLHWTISDGRYRVWHTDRWMM
                     ENPREGEKVLAMLERDPATFRLDKALQAAGARIPDSYAAYEGDVFPDVAQALGREADK
                     GSPNREVFELYRQPVSGTPVIPGSSIKGALRTAWLYHLITAAPDGVQRLAEAVLRKVR
                     ERKTRDPDEDIDALLMGSNGRNPTFSVAKCLHVGDAALVAGSMQVILTDALSEKSDPR
                     ASWFKEWKIFMECIAPGSAVKGRLGIDDGLLREGAQAHGWSREHAALSLDAIWQTAKK
                     SADEVIDMDAVYYRAAGRDDAAEFLADQRKALAEAPASKALISLGMGSGWLKLTLGRL
                     VQKQVSPGDWKTIREAFRLADRSINMQFPKSRKIAVEEEEGERYVYPLGWVWMTYEEV
                     KR"
     gene            complement(2743..3873)
                     /locus_tag="PLT86_11870"
     CDS             complement(2743..3873)
                     /locus_tag="PLT86_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37351.1"
                     /translation="MHMVILTQKSPWLGKGLPHSDTLFGGLCWGIRALEGEQRLVALL
                     EQFAAGSPPFVLSSVFPLLQGKGGETLPLLPQPRGAPKRGSEHDEPQGQRDRAGAIED
                     RAVGKKVRKAEWVTPRMFGDLARGTLNASGIIEKAVFLKDWPEKPGSQPKPEYACADR
                     AILTADDVERLATKPKDGLVIWESVSNARNAIDRVSVETTAFFHEDGIVLGEGVRAYC
                     LLDASEPDVARSVLAAFRWLGERGIGGGASVGYGRFDAAAFEDVSWLPDDPAGNTRVT
                     LSLYHPSVEERAAFAAHPEAVAYEPAIRKGRLESAFVPIPQVWKRSVLYLAEGARFPA
                     IPGRRHYGMNPVVTDEAEGLPDALRFEAQALGFAFTVGVKAA"
     gene            complement(3886..4692)
                     /gene="csm3"
                     /locus_tag="PLT86_11875"
     CDS             complement(3886..4692)
                     /gene="csm3"
                     /locus_tag="PLT86_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOT37352.1"
                     /translation="MEMQYLGNVVLRGKLKCLTGLHVGGSEEKYEIGGMDNPVIKHPL
                     TGYAYIPGSSLKGKLRSLTEWALGMVEIKFNEGERKFEAPAHSCGKVDCAICAVFGCA
                     ILSGKEQKLSGPSRLLVRDSVPTEETRRQMDSLEKNHGLPKVEVKSENSLDRVTSAAN
                     PRPLERVHAGAEFDMELVYGVYDRDGDGGKADIENIGVVLQALRLLEDSSLGGGGSRG
                     SGQVRVHLEATPVVKPVKDYLEGKSAAHAEVKTVPLSEIKKDAYIGAIKL"
     gene            complement(4703..5155)
                     /gene="csm2"
                     /locus_tag="PLT86_11880"
     CDS             complement(4703..5155)
                     /gene="csm2"
                     /locus_tag="PLT86_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOT37353.1"
                     /translation="MNAYQQTNRQGPAQGGHDRPQAPNLAALIRDNGFKHEKTLAVVD
                     DFAAAEFNVGREGQKIRNQLRKLYNQVRAAEAKIGNDEQALKFQVRLLQAQFKYNVSR
                     KAVNAFKPDFVRAFEASTARIRTVEDYRDFVDFFEVLYAYGYSASAEK"
     gene            complement(5152..>5792)
                     /locus_tag="PLT86_11885"
     CDS             complement(5152..>5792)
                     /locus_tag="PLT86_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37354.1"
                     /translation="SISRVATLSSQLDLFFSGYLTGIVEKESKSGYITYSGGDDLYIV
                     AHWQEAIAIAKAIRKEFHKYSCERLTISAGLSLFKQKFPVAQAGENAQAELEEVAKQG
                     NKNACALFGRRLTWEELDEVLREGDELVARIEKGDLARGFPYRMLTLAKQKMNDVRYV
                     GILRYLIRRNVKDSALQQHLENVFGKDKWKEARIPVLCNYTSLRIRRGREEG"
BASE COUNT         1010 a         1906 c         1655 g         1221 t
ORIGIN      
        1 ccagtttggg cctccttcgg agtccagttt gggcctcctt cggagtccag tttgggcctc
       61 cttcggagtc cagtttggac ccgcgggggc ggccagcgcc gaacttcttc gcttgactac
      121 agaataaccg aacgcggggc gcgcggcaag accggggtca aacatgcctc gatcgcgttg
      181 aatggatacc gcgttcgttc tggattcccg cctccgcggg aatgacagga gtcccgcagg
      241 atgcataatc gctgccaaat ggtatccttt ccacgttctg gattcccgcc tccgccgcgc
      301 gcccaggcgc acgggaaatg acgggacgga aaccccgttc agactggctc tttcctgagc
      361 aggtcgagca cctccctgat tgcgcttttc tgggccgccg gattgaacgg gttgtcgtcg
      421 atttcgtcgt cgaacacgga gacgaggttc ctcagcgccg cccgggcaac cgcagcggct
      481 ctttgcacgc gaacagggtc cgggctccgc agcttgtgca ccacgtcatt ccggtggtca
      541 cggatagagt caaccgcaga aaagctgcct gccgcctttt ccacggcgac ccagcgcggg
      601 tcctggcgtc tgtgagcgga tttgatgtgc tcccacaggc tctcttctgg cgattcggga
      661 tgaaggcccg ctttcacact gagctccagc accagcactg ccaggaggag ggcgccgatc
      721 atatcgttgc gggcaagtcg cctttccacc atcaagtacg cgaacacaag gcccgtgcgt
      781 tcgccgtgag cgcgcacgac ctgtagttgt tgcgcggcca gccgcgaaag gggctcaagc
      841 ccgggcgttt gaacgcattc gttccctgcg gtctccagca gttccacgca cccgtcgtag
      901 tccatgcggt catggaggtc ccacgcttcg acgacatgtg caagggccag cagcgccgat
      961 ttctgcggtc cacgctgcat cctgttgccc tggccggcga gtgcccttgc tgcacgggcg
     1021 aaatggcacc cggtcactgc cgcctccacc tcgtccagaa ccgctggaag ccgcaggaca
     1081 tcgatgcgca ccggccacac gggagagtct tccgggtcga gcttcgtggg gtccattacc
     1141 tgccacagac tcgcgttccg caggcgaccg agcatcgaga gcacgaacca ggtctgctgc
     1201 atctgcggcg tgccggacga gatgctgacg aaaagctccg catcttcggg cacatcagcc
     1261 agcgccgcgg ggaccacctc gcccatggcc tgaagtacat cagtcgcatc ggcggggttc
     1321 tccggagcga tgggatgaat ggccaccctg tcgcgcggaa agccagtctc ctccagccgc
     1381 ttcattacgt ccgggatttt ggtggtcgcc gttccttcct cgcctgcgga gggaaacaga
     1441 agcagcagat caggctcaac gatccgcaac gcggtgagca gaggcccttc gctgttctga
     1501 ccggtccttt tgtccaccga cccaaacgga tcacggttgc cgacgcagga aagcatcacc
     1561 ctcatcgctt cacctcttcg tacgtcatcc acacccagcc gaggggatag acgtatcgtt
     1621 cgccttcctc ctcctcaacg gcgatcttgc ggctcttcgg gaactgcatg ttgattgagc
     1681 ggtccgcgag gcggaatgct tcgcggattg tcttccagtc gcccggggat acctgtttct
     1741 ggaccagtcg cccgagcgtg agtttgagcc accccgaccc catcccgagc gagatcaaag
     1801 ccttgctggc gggcgcttcg gcgagcgctt tcctctgatc ggcaagaaac tcggccgcgt
     1861 catcccgtcc cgcggcgcgg tagtacacgg cgtccatatc gatgacttca tcggccgatt
     1921 tcttcgccgt ctgccagatc gcatccagag acaaagcggc gtgttcacgg ctccagccat
     1981 gcgcctgcgc accttccctc aacagcccgt cgtctatccc cagcctgcct ttgacggcgc
     2041 ttcccggagc gatgcattcc atgaagatct tccactcttt gaaccatgaa gcgcgaggat
     2101 cgcttttttc cgaaagcgcg tcggtaagga tgacctgcat gctgcccgcc acaagggcgg
     2161 catctcccac gtgcaggcat ttcgccacgc tgaacgtggg atttctcccg ttcgagccca
     2221 tgaggagtgc gtcgatgtcc tcgtcaggat cgcgcgtttt gcgctcacga accttcctca
     2281 ggacggcctc tgcgaggcgc tgaacgccgt cgggagcggc ggtaatcaga tggtacaacc
     2341 acgccgtccg caacgcacct ttgatgctgg agccgggaat gacgggcgtg ccgctcacgg
     2401 gttgccggta cagctcaaac acttcccgat tggggctgcc tttgtccgcc tctctgccga
     2461 gagcctgcgc cacgtcgggg aaaacgtccc cttcgtacgc cgcgtatgag tcgggaatcc
     2521 gcgctcccgc cgcctgcaat gctttgtcca gccgaaacgt tgccgggtct cgctccagca
     2581 tggcgagcac cttttcgccc tcgcgcgggt tctccatcat ccatcggtcg gtatgccata
     2641 cccggtagcg gccatctgag atcgtccagt gcagcggacc cactttctcc ccggttccga
     2701 tgtgcaccgg cgttacgggc tcgatcgtaa gggtgtactg tttcatgccg cttttacccc
     2761 cacggtgaag gcgaaaccga gcgcctgcgc ctcgaaacgg agcgcgtctg gaagaccttc
     2821 cgcctcgtcc gtcacaaccg ggttcatgcc gtaatgccgc ctgccgggaa ttgcggggaa
     2881 ccgcgcgcct tcggcgaggt agagaaccga gcgtttccac acctgcggga tcggaacaaa
     2941 cgcggactcc aggcgccctt ttcgaatcgc cggttcgtat gccaccgctt ccgggtgcgc
     3001 ggcgaaggcg gcgcgttctt ccacggacgg gtgatagagg gacagcgtca cccgtgtgtt
     3061 ccctgccggg tcgtccggca gccagctcac gtcctcgaat gcggcggcat cgaacctgcc
     3121 gtacccaaca ctggcgcccc cgccgattcc gcgttcgccc agccaccgga acgccgccag
     3181 aacagaccgg gcgacatccg gttcggaggc gtcaagaagg cagtatgccc ggacaccttc
     3241 gcccagcacg atcccgtcct cgtggaagaa cgccgtcgtc tccacggaaa cccggtcgat
     3301 ggcattccgc gcgttggaaa cgctctccca gatgaccaaa ccgtccttcg gcttcgttgc
     3361 cagccgttcc acgtcgtccg cggtaagaat cgctctgtcc gcacaggcgt attcgggctt
     3421 cggctggctt cccggttttt ctggccagtc tttcaggaac accgcttttt cgatgatccc
     3481 gctggcgttg agcgttcccc gggcgaggtc gccaaacatc cgcggcgtaa cccattccgc
     3541 cttacgcacc ttctttccga ccgcgcgatc ctcgatcgcg ccagcgcgat cacgctggcc
     3601 ttgaggttca tcgtgttcgc ttccgcgttt tggtgccccg cgcggctggg gcagaagcgg
     3661 cagcgtttcg ccgcccttgc cctgcagtag cgggaacacc gagctgagca cgaagggagg
     3721 ggatccggcg gcgaactgct ccagcagcgc cacaagccgc tgttcgcctt cgagggcgcg
     3781 gatgccccag cagagcccgc cgaacagggt atccgagtgc ggcaggccct tcccgagcca
     3841 cgggcttttc tgggtgagaa tcaccatgtg catggggcgt ccccttcaga gcttgatggc
     3901 accgatgtag gcgtccttct tgatctcgct gagcggcacg gtttttacct ccgcgtgagc
     3961 agccgatttg ccctcgaggt agtccttcac cggtttcacg accggcgtgg cctcgagatg
     4021 aacgcgcacc tgccccgaac cacgggaacc gccgccgccg agggaggaat cctccagcag
     4081 cctcagcgcc tgcaacacca cgccgatgtt ctcgatatcc gctttgccgc cgtcgccatc
     4141 gcggtcgtac acgccataga cgagttccat gtcgaattcc gccccggcgt gcacgcgttc
     4201 gagtggacgc gggtttgccg cagacgtcac tctgtccaag gagttctcgc ttttcacctc
     4261 gaccttgggc aatccatggt ttttctcgag actgtccatc tggcgtctcg tctcttcggt
     4321 gggaacggag tcacgcacca gaaggcgaga aggaccggac agtttctgct cctttcccga
     4381 gagtatcgcg caaccgaaaa ccgcacagat ggcacaatct accttcccgc atgagtgcgc
     4441 aggggcttcg aatttccgtt cgccttcgtt gaatttgatt tcaaccatgc caagcgccca
     4501 ttccgtaagc gaacgcaatt tgcccttgag cgaagagccc gggatgtacg cataacctgt
     4561 caatggatgc tttatcacgg ggttgtccat tccgccgatt tcgtattttt cttcggatcc
     4621 accaacgtga aggccggtca ggcacttaag tttgcctctg agcacgacgt tcccaaggta
     4681 ttgcatctcc atgtcgttcc ccctatttct ccgctgaagc ggaataaccg taggcgtaaa
     4741 gaacctcaaa aaagtcgaca aaatcgcggt aatcctcgac agtcctgatc cgcgcggtgc
     4801 tcgcctcaaa cgcccttacg aaatccggct tgaatgcgtt gactgccttg cggctgacgt
     4861 tgtatttgaa ctgagcctgc aggagcctga cctggaactt cagagcctgt tcgtcgttgc
     4921 cgattttggc ttcggcggcg cgtacctggt tgtaaagctt gcgcaactgg ttgcgaatct
     4981 tctgcccttc tcttcccacg ttgaattccg cggcagcgaa gtcgtccacc accgctagcg
     5041 tcttttcgtg cttgaacccg ttgtctcgaa tcaaagccgc cagattcggc gcctgtggtc
     5101 gatcatggcc accttgcgca ggaccttgcc ggttggtttg ctggtacgcg ttcatccttc
     5161 ctcccttcct ctgcgaatgc ggagactcgt gtagttgcac aaaacgggta tcctggcctc
     5221 tttccacttg tccttgccga aaacattctc aaggtgctgc tgaagcgccg agtccttcac
     5281 gttgcgacgg atgagatacc gcaagatgcc cacgtagcgc acgtcattca tcttctgctt
     5341 cgccagtgtc aacatgcggt agggaaagcc ccgtgcaagg tcgcctttct cgattcgcgc
     5401 aacgagttcg tcgccttctc tcagcacctc atcgagttct tcccacgtca ggcgccggcc
     5461 aaacagcgcg caggcattct tgttcccctg cttcgccacc tcctccagct ctgcctgcgc
     5521 gttctccccg gcctgcgcca cggggaactt ctgcttgaac agcgacaagc cggcggagat
     5581 cgtcagtctt tcgcacgagt acttgtggaa ttccttgcgg atggccttcg cgatggcgat
     5641 cgcttcctgc cagtgggcga cgatgtagag atcgtccccg ccggagtacg tgatatagcc
     5701 ggacttgctc tctttctcca ctattccggt gaggtaaccg ctgaagaaca ggtcgagctg
     5761 gctgctcagc gtggccacgc gggagatgga cg
//
LOCUS       DAOSOO010000198         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14177_length_5736_cov_5.016018, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000198 DAOSOO010000000
VERSION     DAOSOO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14177_length_5736_cov_5.016018"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1425
                     /locus_tag="PLT86_11890"
     CDS             172..1425
                     /locus_tag="PLT86_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37355.1"
                     /translation="MAMCWRATGDERYLADAQTVWERVAQDVFVENGQIFEGCGPCNG
                     YSMISYEYLLRYRIATEDDSLDPVIERSTDWVTRMYTDRYTTFRGVSTRQDIPDATHK
                     AVPLLLGLERLSSRAPWYSVMADSLLDRIERGYETAPAQHGGIAWVAAAAAHNSATSN
                     GSNLAARRVPSVRSYSSQATSYTTVEQPGYQALIAFRSLSPRKGLQTWAVRGEAPFIF
                     PEDNQPSCAISWGHDSARDNVTEWDTIREDAGQFPSFTTRTGDAVTSYVVTPMTLVVV
                     YSFDTPRETLIRWCASSRHTGGFHLQNGFVSAIGAKGSLNWWSGEPTISEDGFVVEFR
                     GDLLTQAFAFSAGGFRRIGSIASEDGLITLSWEDDAGSYAAVANHAPRPLPLPPDAKR
                     PQNEHATLLDPCEVRVFCNDRSPVQ"
     gene            complement(1466..2401)
                     /locus_tag="PLT86_11895"
     CDS             complement(1466..2401)
                     /locus_tag="PLT86_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37356.1"
                     /translation="MTPKERAVAALERRRPDDIVPTFELVYFLGPEKFGRDWTENIRS
                     MTPKEQDRYLHEHAELHVMIAEEYDYSILRSDYIEAIRIFKKWGLDKTYLLCGEADGT
                     MAIPNGHDMVEVAAALRERPDEMHAQMRRGAEGAKEAGRRFREAGAEAVTMCADYCFN
                     DGPFLSPGMFREFVTPYLTEIIASHRENGLFVIKHTDGNIMPILDQLVEANPHALHSI
                     DPQARVDLAEVKRITAGKICLCGNVNCGLLQTGTDQEVREDVLRSLRDGMPGGGYIFS
                     TSNCAFRGMPLERYELMMSLRKEYGRYDKPAFPMA"
     gene            2539..3375
                     /locus_tag="PLT86_11900"
     CDS             2539..3375
                     /locus_tag="PLT86_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT37357.1"
                     /translation="MSRKVVLPTWYYRQYPSDFRHGEEATLGILGWARRDCTVPLDEM
                     ALVLMHFWNVGFPGGPEWSENSPHVLVRQAQEYVGRCVAQFTRSMPPILKAARESGMT
                     IVHVASGEDYAKRYPHYQRTCAEVNLEAPQKQPGAIRPQTTDIPTNVERYGPGWADFK
                     DAPYDFPSIVRPEAENELLCVHTHQLNSVLRNRGIWHLTYCGFAINWCLWHSPCGMVD
                     MSRLGYTCGCIRQGVVAVENKESVRTRGNHDYAMWKTAHMFGFVIDDDDFIRGVQNTR
                     EG"
     gene            complement(3424..4785)
                     /gene="gdhA"
                     /locus_tag="PLT86_11905"
     CDS             complement(3424..4785)
                     /gene="gdhA"
                     /locus_tag="PLT86_11905"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT37358.1"
                     /translation="MASFVDEIMAQVIAKNPTEKEFHQAVTEVAESVALVYDKFPEFR
                     KAKVFERMVEPERVIMFRVPWVDDKGEIQINRGFRIQMNSAIGPYKGGLRFHPSVTLG
                     VLKFLAFEQVFKNSLTTLPMGGAKGGSDFDPKGKSDGEVMRFCQNFMNELFRHIGHDT
                     DVPAGDIGVGGREIGFLFGQYKKLANQFTGVLTGKGPAWGGALIRPEATGYGATYFAA
                     EMLATRGEKLEGKTVLVSGSGNVAQYTVEKVNDLGGKVVTMSDSSGYVYDEAGIDAKK
                     LKFIMELKNERRGRIKEYADAFPGVVYTPVDPNLGYNPLWNHKADVAFPSATQNEINE
                     RDAENMVRNGVICVAEGANMPTTLEGVRVFLDAGILYAPGKAANAGGVAVSGLEMSQN
                     SMRLPWTREEGDNRLRGIMKSIHKTCATTAEQFGTPGNYVNGANIGGFMKVGRAMLDQ
                     GIV"
     gene            complement(4977..>5736)
                     /locus_tag="PLT86_11910"
     CDS             complement(4977..>5736)
                     /locus_tag="PLT86_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37359.1"
                     /translation="AGFSGGRKAVCPGLAGVDTMRVLHGPEILSHPNVREGCLAGNPF
                     HEEATEIARRAGLDFTVSVTLNEQRRITGVYSGDPESAHAAGCAAAARNTTAQIPEPV
                     DAVITTSAGYPLDLTFYQSVKAMTAALPILKKGGTLIVASSCDEGIGSPDFTELMLGT
                     PSLKEFRDRLKDPGFFVPDQWQFQMMCRALETAQVLYFSTGIPADILAKLFVTPIASV
                     EEGIARALERHGSGASMAIIPEGPYVMPAVFPSR"
BASE COUNT         1155 a         1671 c         1622 g         1288 t
ORIGIN      
        1 taaatggcat ggcgactgga gtgtcttctc accgcatttc tgtgtgtcag agaggcgctg
       61 acttccgagg aacggtcccg ataccgggac acgctcttgc gcgcgatgga gttccttcga
      121 gagactccgt gcgaacaccg ttgtaaccgg ggtgtggtct ggtgtgccgt gatggcgatg
      181 tgctggcgcg ctaccggtga cgaacggtac ctcgcggacg cgcagaccgt gtgggaacgt
      241 gtagcccagg atgtgtttgt cgaaaacggg cagattttcg aaggttgcgg gccgtgcaac
      301 ggctactcca tgatttcgta cgagtatctc ttacggtacc gcatcgccac ggaagacgac
      361 tccctcgacc ccgttattga acgctccaca gactgggtta cacggatgta caccgaccgg
      421 tatactacgt tccggggcgt atccacgagg caggacatac cggatgctac gcataaagcg
      481 gtcccattgc tcctcggttt ggaacggttg tcgtcgcgcg ctccctggta ttccgtaatg
      541 gctgacagtc tgttggaccg gatcgagcgt gggtacgaaa cagcgccggc acaacacggt
      601 ggaattgcct gggtggccgc ggcagcggcg cacaattctg cgacatcgaa cggaagtaat
      661 cttgcggcca ggagagtacc gtccgttcgt tcgtactcat cccaggctac cagttatacc
      721 acggttgagc aacccggtta ccaggctctc atcgcgttcc gcagcctttc cccgcgaaaa
      781 ggcctccaga catgggctgt tcgcggtgag gcccccttca tcttcccgga agacaatcaa
      841 ccatcgtgcg ccatatcgtg ggggcacgat tctgcccgag acaatgtgac cgaatgggac
      901 accatccgtg aggatgcggg ccagttcccg agtttcacca cgaggacggg agacgccgtc
      961 acgagttatg tcgtcacgcc aatgactctc gttgtggtct attccttcga cacgccccgc
     1021 gagaccttga tcagatggtg cgccagcagc cgccacaccg gagggttcca ccttcagaat
     1081 ggcttcgtga gcgcgattgg agcaaaaggt tcgttgaact ggtggagcgg cgaacccaca
     1141 atcagtgagg atggattcgt tgtcgagttt cgaggcgatc tcctcacgca ggcgttcgcg
     1201 ttctctgccg gcggcttccg gcgaattggt tccattgcgt ctgaggacgg tctgatcaca
     1261 ctgtcctggg aagacgacgc gggcagctac gctgccgtcg ccaaccatgc gccacgtcca
     1321 ctgcccctgc caccagacgc aaaacgcccg caaaacgaac atgcaacgct gttggacccc
     1381 tgcgaagtga gggtcttctg caacgaccgc agtccagttc agtagtccac cgtcaaaggc
     1441 aagacggaac gtcggatcta ttcgatcagg ccatcgggaa tgccggtttg tcgtaacgcc
     1501 cgtattcctt ccttagcgac atcatcaact cgtaccgttc gagcggcatg cctcggaatg
     1561 cgcaattcga agtcgagaaa atatacccgc ccccgggcat cccatcacgc aaggagcgca
     1621 agacgtcttc gcgcacttcc tggtccgtac ctgtttggag gagcccgcaa ttgacgttcc
     1681 cgcagagaca tatcttcccg gcagtgatgc gcttgacttc ggccaggtcc acgcgcgcct
     1741 gcgggtctat ggaatggaga gcatggggat tcgcttcaac cagttggtcc agtatcggca
     1801 tgatattgcc gtcggtgtgc ttgatcacga acaggccgtt ttccctgtgt gacgcgatga
     1861 tttcggtgag atacggcgtg acgaactcgc ggaacattcc gggactcaga aacgggccgt
     1921 cgttgaaaca gtaatcggca cacattgtca cggcttcggc tccagcctcg cggaaccgtc
     1981 gccccgcttc tttcgcgcct tccgcgcctc gtcgcatctg ggcgtgcatc tcatcaggcc
     2041 gttcccgcag tgctgcggcg acttccacca tgtcgtgccc gttcggaatc gccatcgtgc
     2101 cgtccgcttc gccgcagagc agataggttt tgtcaaggcc ccatttcttg aaaatccgga
     2161 ttgcttctat gtagtccgag cggagtatgg aatagtcgta ctcctccgcg atcatcacat
     2221 gaagctcagc atgttcgtgc aggtaacgat cctgctcttt tggcgtcata ctccggatgt
     2281 tttcggtcca gtcccgtccg aacttctccg gtccgagaaa atacacgagc tcgaacgtcg
     2341 gcacgatgtc atccgggcgc cgacgctcca aagccgctac ggcgcgctcc ttaggggtca
     2401 tgatggtgtt tctccttgtt gctggtgcga cacgaccttg gtgcccgtgt cctcgggact
     2461 atcagagttt tccgttacct tgtgtacaca aggtacggat gctcgccgtc ggacgaaacg
     2521 cacgacaagg aggaaggcgt gagtcgaaag gttgtattgc ccacctggta ttaccgtcag
     2581 tatccctcag attttcggca cggcgaggaa gcgacgctcg gcatactggg atgggcaagg
     2641 cgggattgca cggtgccgct tgatgaaatg gcgcttgttc tgatgcactt ctggaacgtg
     2701 ggttttcctg ggggaccgga atggagcgaa aactctccgc acgtgctggt aaggcaagct
     2761 caggagtacg tggggcggtg cgttgcgcaa ttcacgcgca gtatgccccc catactcaaa
     2821 gcggcccgtg agtcgggtat gacgatcgtg catgttgcct cgggggagga ctatgcaaag
     2881 cgttatccgc attatcaacg aacgtgcgcg gaagtgaatc tggaggcccc tcaaaagcag
     2941 cccggcgcca tccgccctca aacgaccgat attccgacaa atgttgagcg gtatggaccg
     3001 gggtgggctg atttcaaaga tgcgccgtat gatttcccgt ctatcgtgcg gccggaagcg
     3061 gaaaacgaac tgctttgcgt gcacacccac cagctcaaca gtgtcctgcg aaatcgtggc
     3121 atatggcatc ttacttactg cggatttgcc ataaactggt gcctctggca cagcccgtgc
     3181 ggtatggtcg atatgtcacg cctcgggtat acgtgcgggt gcatccgaca gggggttgtc
     3241 gccgtggaaa acaaagaatc ggtgcgcacg agaggaaatc atgactatgc gatgtggaaa
     3301 acggctcaca tgtttggctt cgtgattgac gacgacgact tcatccgtgg tgttcagaat
     3361 acgagagagg ggtagaagtc ttccacccct ctcctggcat cagtgtctgc ggtctgcgat
     3421 ggatcagacg atcccctggt ccaacatcgc tcttcccact ttcataaacc cgccaatgtt
     3481 cgcgccgttc acgtagttcc cgggagttcc gaattgctcc gcggtcgtcg cgcacgtctt
     3541 gtgaatgctt ttcatgatcc cacgcagccg attatcgcct tcttcgcggg tccacggcag
     3601 tcgcatgctg ttctgagaca tctcaagtcc ggaaactgct actccgcccg cgttcgcggc
     3661 tttgccgggg gcgtagagaa tcccggcatc aaggaagaca cgaacgccct ccagagtggt
     3721 cggcatgttg gctccctctg cgacgcaaat gacgccgttg cgcaccatgt tttccgcgtc
     3781 tcgttcgttg atctcattct gggtcgcgct ggggaatgca acgtctgcct tgtgattcca
     3841 aaggggattg tacccgagat tcggatccac gggagtgtac acgacgccgg ggaacgcgtc
     3901 tgcgtactcc ttgattcttc cgcgccgttc attcttcagt tccattatga acttgagctt
     3961 tttcgcgtca atccctgcct cgtcgtacac gtaccccgag gaatctgaca tggtgaccac
     4021 tttaccaccg aggtcattta ccttttccac cgtgtactgc gcaacattgc cgcttccgga
     4081 aacaagaacc gtcttgcctt ccagtttctc tcctctcgtc gccagcattt cggccgcgaa
     4141 atacgtggca ccgtacccgg ttgcttccgg acgaatgagg gcgcctcccc acgcaggtcc
     4201 tttgcccgtg agaacgccgg tgaactggtt cgccagcttc ttgtactgac cgaaaaggaa
     4261 gccgatttcc ctgccgccta cgccgatgtc gcccgccgga acgtccgtgt cgtgaccaat
     4321 atgccggaac agctcattca tgaagttctg gcagaaccgc atcacttctc cgtcgctttt
     4381 cccttttgga tcgaaatcgg aaccaccctt cgcgccgccc atgggcaagg tggtcaggct
     4441 gttcttgaac acctgctcaa aggcgaggaa tttcagcacg ccaagcgtta ctgaagggtg
     4501 gaaccgcaat ccgcccttgt agggcccgat cgcgctgttc atctgaatcc tgaagccccg
     4561 gttgatctgg atctcgcctt tgtcatcaac ccagggaacc ctgaacatga tcacgcgttc
     4621 cggctcgacc atgcgttcga atactttggc cttgcggaac tccgggaact tgtcgtagac
     4681 gagtgcgacc gattctgcga cttcggttac ggcttgatgg aattccttct ccgtggggtt
     4741 tttcgctatg acctgagcca tgatttcgtc tacgaaactc gccatctttt agtctcccgg
     4801 ggttcgttgt tgaagtgtta ttgacaaagc aagaataaat atccggattc gcccagtttt
     4861 gccgcaaggg tctgccaacg gtgcttctgg ctattaatgg cacgaaaatg gagtggcgga
     4921 accgtagtgt cgcgaatcgg tcaggcggaa tgagaacgaa tatcgagggg aatcaatcaa
     4981 cgggacggaa agacggccgg catcacataa ggaccttccg ggatgattgc catgcttgct
     5041 ccggaaccat gtcgctccag agcccgcgca atcccttcct ccacggatgc aattggcgtg
     5101 acgaacaact tcgccagaat atcagcaggt attccagtcg agaaataaag cacctgtgct
     5161 gtttccagcg cgcggcacat catctggaac tgccactgat ccggcacgaa gaatccgggg
     5221 tccttcaatc ggtcgcggaa ctctttcagg gagggagttc caagcatgag ttccgtgaaa
     5281 tccggagatc cgattccttc atcgcaggat gaagccacga tcagcgtccc gcctttcttg
     5341 agtattggca gagcagcagt catggccttc acggattggt aaaacgtcag atccagcggg
     5401 tatcccgccg aggtggtgat gacggcgtcc acaggttcag gaatctgcgc agtggtgttc
     5461 ctggccgcgg cagcgcaccc ggcagcgtgc gcagattcgg ggtcgcccga atacactccg
     5521 gtgattctgc gttgttcatt gagcgtcacc gaaacagtga aatccagtcc tgcccggcgc
     5581 gcgatttcgg ttgcttcctc gtggaacgga ttcccggcga gacacccttc gcggacattc
     5641 gggtgcgaga gtatttccgg gccatgcaga acacgcattg tatcgacccc cgccaacccg
     5701 gggcaaacag cctttctccc gccgctgaat cccgcc
//
LOCUS       DAOSOO010000199         5721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14228_length_5721_cov_4.339569, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000199 DAOSOO010000000
VERSION     DAOSOO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14228_length_5721_cov_4.339569"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLT86_11915"
     CDS             complement(<1..567)
                     /locus_tag="PLT86_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT37360.1"
                     /translation="MPRIFDNIENDLLPALQDTMRVAQRGDFCVGYFNLRGWKHLAGY
                     VEQWQGGDGACCRLLLGMQPRPSEVLFRVFREQTIDNKTAVRLKTEVATELRQQLARG
                     IPNDEEETALRRMVRQIRAGKLIVKVFLRHPLHAKLYMLHREDVNNPITAFLGSSNLT
                     LAGLVNQGELNVDVLDHDACAKLQGWFED"
     gene            complement(1456..2784)
                     /locus_tag="PLT86_11920"
     CDS             complement(1456..2784)
                     /locus_tag="PLT86_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOT37361.1"
                     /translation="MTTPHTPHSVETEHRVLGAMMMDCQAVETAAAVLDDGAAFHSIR
                     ARTLYRYMVSTADSGKPVTLDQLAQDVARIGLKMEGHDATVWLGDVGASVGTSVHVKH
                     DANRVLDYHLRRQLKTLAWRIQTAADDPEADVREIVAMVERTIIDNVPSEEFEVFSLE
                     DCVSQAFEAMEKRARCCRKYAGASSGYDELDMITGGFRDGEFIVIAARPSVGKTAFAL
                     NLFRNMSAQGIPAAFFSLEMARDPVTTRLLSSVSQVEEHRLNTGEVLARGDDEDAIAL
                     RKAGEILSALPGFIGDPGKNMTCTQIRAKARRLVHSPDTKVRAVFIDYLHLIEPTERT
                     DNRVQELERISKNLKALARELNVPVVVLSQLNRETEQSGRKPKLHNLKGSGAIEQDAD
                     IVIMLDRDDRRGNSEPGFLNLTVEKNRNGRTGECSLRCRPDIQLVESLKD"
     gene            complement(3941..4198)
                     /locus_tag="PLT86_11925"
     CDS             complement(3941..4198)
                     /locus_tag="PLT86_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37362.1"
                     /translation="MSVESSHTKGMKDSVESRSIPRLIPVTKWNEHHEWPPIGGLRHL
                     VFHEGSNGFHSVIRRVGRRVLIDEQAFFTWVDRQGSQGGAQ"
     gene            complement(4371..5051)
                     /locus_tag="PLT86_11930"
     CDS             complement(4371..5051)
                     /locus_tag="PLT86_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37363.1"
                     /translation="MAPVPEEETYEWWLKQPTWELHDAITLTDRKYGWIPTPRKPGDT
                     AKIMAYRAFPTDYRRFVLAKLEAGEVPIHHTDGDEIHVVPIQFLFWRLTWTEFAGPLA
                     KPLRDCVAENLLNLMETGQLLPPPAAKKLIRHTKRRRPRLDLVNKYAGPQAQLLGAAL
                     ACLAAFRDECCDPDGRVNVTHLLNQMNSKAQLFWDTGDKPDDGWDVPQHHDTVEKLLR
                     EWLRKTGG"
     gene            complement(5282..>5721)
                     /locus_tag="PLT86_11935"
     CDS             complement(5282..>5721)
                     /locus_tag="PLT86_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOT37364.1"
                     /translation="KNAEVRIVPLKSHARGALAELWKDRLSDSALVFPAPTAPEGSSK
                     SIDIESAWRSALKRAELKNFRFHDLRHSAASYLAMNRGSPLDIAAVLGHKTLQMVKRY
                     AHLSETHTSAVVERMNVAIFDGSSCDGREPGRPEATDQGRTPR"
BASE COUNT         1115 a         1650 c         1615 g         1341 t
ORIGIN      
        1 gtcttcaaac catccttgga gcttggcgca cgcgtcgtga tcgaggacat caacgttgag
       61 ttcgccctgg tttaccagcc cggccagagt gaggttgctg cttcccagga acgcggtgat
      121 gggattgttc acgtcctcac ggtgcagcat gtagagcttg gcgtggagag gatggcgcag
      181 gaacaccttg acgatgagtt ttcctgcccg aatctgcctc accatccggc gcaacgcggt
      241 ctcttcctcg tcgttcggga tgccacgtgc cagttgttga cgcagttctg ttgcgacctc
      301 ggttttcagg cggactgcgg ttttgttgtc gatggtttgc tcgcggaaaa cccgaaacag
      361 tacctccgac ggtcgaggtt gcattccgag caagagtcgg caacaggccc cgtcgccacc
      421 ctgccactgc tcaacgtatc cggcaagatg cttccaaccg cgaaggttga agtacccgac
      481 gcagaagtcg ccccgttgag caacacgcat cgtgtcctgc agggcgggca gaagatcgtt
      541 ttcgatgttg tcaaaaatgc gcggcatacc acctccccag ccgtatgtac caccacattc
      601 tgttctaagg tcaccaaatt cttgcagatg ggcaacccgg tctttccagc agaacctcag
      661 ggggtccggg aacccctgaa ggcgagcttt caatcgttcc gttcctgctt tccttaggag
      721 ggaaaaccag ccgcgggaac gggaacgctg ttccatttcg ggaaatccac gcctggcatg
      781 cgcgggcggg gggtcggtgg agacagctct cacacagaac agtcgcgaac aggcgcgaca
      841 cgggaactcc tcctgcgacc aacctgtccc gcgtttcgca gcaacggtgc ctccccattc
      901 gggttcgcaa gagacaacgg aaaggcaacc gtgtctaact ttctgtgaaa aatatactta
      961 cgcaggcaaa gcgttgccga tgcgaaacgc ggtgttgttt tcccgtgtac gcctatttat
     1021 ggccgtggaa gggtgccgga gacgaaactg ggcacggatg ataagaacgg ttatgtcaac
     1081 ttagtgtggg gatctgagcg gggaggtatc ggcggagcgc atgctcggat tacccgtatg
     1141 tcaactgcct cgacgaactg aagctcggtc gagggcgccg ggaaccgagc gtatcccgat
     1201 gccccccaat gggcccgcct ttgcgcacag gtggggtatg atttgatggc tgttcagcct
     1261 gtctgccgct cggattgcgt gatggagcac tggttgagca cggaaggctc gccgagtgaa
     1321 ctcctgttgc tccgaggtcg gcgtcccgcg gggtccacca actgaaggga cttgacaaac
     1381 cgagtcgttt gccaaatgac caactgcata agtgtgccgc cgtacccacg gcgttccgat
     1441 taggcatcgt tgacctcagt ccttgaggga ctctacgagc tgaatatccg gacggcaccg
     1501 aagtgagcac tccccggtcc ggccgttccg attcttctcc acggttaggt tcaggaagcc
     1561 cggctcgcta ttccctctgc gatcgtctcg gtcaagcatt atcacaatat cggcgtcctg
     1621 ctctatcgcg cctgagccct tgaggttgtg cagtttgggc tttcggccgc tttgttcggt
     1681 ttcacggttc aattgcgaga gcactaccac gggcacgttc aactcgcggg caagtgcctt
     1741 caggttcttg gatattcttt ccagttcctg gacacgattg tctgttcgtt ccgttggttc
     1801 aatgagatgc aggtagtcga tgaacaccgc ccgcactttg gtatcgggcg aatgaaccag
     1861 gcgcctcgcc tttgcgcgga tctgcgtaca cgtcatgttt tttccgggat ctccgatgaa
     1921 tccaggcaat gcggaaagga tttcgcccgc ttttcgaagc gcgatggcgt cttcatcatc
     1981 cccacgtgca agaacttcac cggtgttgag tcggtgctct tccacttgtg aaacgctact
     2041 cagcaaacga gtggtcacgg ggtcgcgtgc catttccaaa gaaaagaacg ccgctgggat
     2101 gccctgtgcg ctcatgtttc ggaacaggtt caaggcaaat gctgtttttc ctacggaagg
     2161 acgcgcagcg ataacaatga actcgccatc acgaaagccg ccagtgatca tgtccagttc
     2221 atcatatccc gacgaagcac ccgcgtattt gcgacagcaa cgtgctcgct tttccatggc
     2281 ttcaaacgcc tgcgacacgc aatcctcgag actgaacact tcgaattcct ccgaaggcac
     2341 gttgtcaatg atcgtccgtt ctaccatcgc cacaatctct cgcacgtcgg cttctgggtc
     2401 gtctgctgcc gtctggattc tccatgcgag agtcttcagt tggcgccgca gatggtaatc
     2461 caacacgcgg ttggcgtcat gtttgacgtg cacggaggtg ccaaccgaag caccgacgtc
     2521 gcccaaccat accgtggcgt cgtggccctc catcttgaga ccaattcgcg cgacatcctg
     2581 agcaagttgg tcgagggtca cgggtttccc tgagtccgcg gtgctcacca tgtagcggta
     2641 caaagtgcgc gcacgaatgg agtgaaacgc tgcaccgtca tcgagaacag ccgccgcggt
     2701 ttcgacggct tgacagtcca tcatcatcgc gcccagtacc cggtgttctg tttccacgct
     2761 gtgaggcgtg tgcggtgttg tcatcgcgtt ttccatgcta ggacgtcctt ccttttttct
     2821 gcgattcgtt catcggcctt tcgagcgagc atttccctct cttccttcgc tcggtctgcc
     2881 cgcggttcca ttgtcagccc gatccacggg tgcctcttaa gccagttctg tggtttcggg
     2941 aaataatcga ggtcgtcgca tgtgtatacc gattcacgca gtgcggcgag cacgttgtcc
     3001 aagttgaatc gggggtctga acgttctttt tggaactgct cccaagcggg tacgaagttc
     3061 ccctttttgt agcgacaatc ggggtattcg tcccagaacg ccacgaacat cgaatcctct
     3121 cccgggtcgg ccatttttgg cggcgctttc ggtttggaga gggcgcaaac aacgcgcttg
     3181 tctggtgcct cagctttcga agaaccgaat gggattggtt caatacatct aggttcatca
     3241 ttattagggg gtacaggggg acgggtaaca tcggcatttt ggtcgcgtaa caatggacct
     3301 ttggtcgttc tgaacctgaa tcggtccgtg attgtataga ccgttggcag cgcaccgcgg
     3361 cctttgcccg ctcctccatg acgtcgttgc cttcccaata ccccgagcct gacgaggtgt
     3421 tccagcgcgt tcgcgacagt gcggtggccc agcttgagtc tttttgcgat ctccgccgtt
     3481 ttgggccaag cacgcccttc agcgtctgcg taggatacaa tacggaagaa gaccgcttcg
     3541 aggacggcgt tcgacggatc aaaacgaccg cgcacgtatg cccccatcgt gccgtaaaac
     3601 gcaatgtggg cttcccagaa cggtcgcgtt ttggactccc gttccgacat cggttcgagg
     3661 ggcaccctgt ttcccaggtt ggcacccgca gtcgacttgt ccgcatcgct gacacccatt
     3721 cagcgagacc tcttcactgg ctccgattca tggtgtgccc atgtgcggca caccacacga
     3781 aatggacaaa cctgccttgc ttgagtcctt cgccggtacc tgtgcgaagg atcaacgcca
     3841 ccctccaccc aggcaccgac gcgcgtgggt ggagggaaac gaggcaccca cgaggcgtga
     3901 ggttccttac gggggataaa gcaccagaag cgcgagttgt tcactgcgct cctccttggc
     3961 ttccttgccg gtcgacccag gtgaagaagg cctgttcgtc gatgagcacc cggcggccga
     4021 cgcggcgaat gactgaatga aagccgttgc tcccttcgtg gaacacgaga tggcggaggc
     4081 caccgatggg gggccattcg tggtgctcgt tccacttggt gacggggatg agacggggga
     4141 tgctgcgact ctcgacagaa tctttcatcc ctttcgtgtg acttgattcc actgacatgg
     4201 tgttcccttt ccgtattcac ttcggccacc atggccgaat gtgacattcg aaaagaaagc
     4261 gatggtttat cgattgtcaa cagaagtaac tgaatgtaac cggggacatt aagaggcagc
     4321 ttgaggggta ccttaattcg tcggttagag gaccggcccg cggaggggtc tcaaccgccc
     4381 gttttccgga gccattcccg gaggagtttc tctaccgtgt cgtgatgctg agggacatcc
     4441 caaccatcgt ctggcttgtc gccagtgtcc cagaacagct gcgctttcga gttcatctgg
     4501 tttagtagat gggtgacgtt tacgcgaccg tcagggtcac agcattcatc gcggaaagca
     4561 gcgaggcacg caagcgcggc gcccaacaac tgggcttggg gcccagcgta tttgtttacg
     4621 agatcgagac gcggccttct tcgttttgtg tgtcgaatga gtttctttgc ggcgggcgga
     4681 ggtagcaact gccccgtttc catcagattc aataggtttt cggccacgca atcccgaagt
     4741 ggtttggcaa gtggacccgc gaattcggtc cacgtgagcc tccagaacag gaactggatc
     4801 ggtacaacgt gtatctcgtc tccgtcggtg tggtgaatcg ggacttcgcc tgcttcaagc
     4861 ttggcaagaa cgaatcgtcg gtagtcagtg gggaaagctc ggtaggccat gattttcgct
     4921 gtgtcgccag gttttctcgg cgtcggaatc cacccgtatt tcctgtccgt cagcgtgatc
     4981 gcgtcgtgca actcccaagt cggttgcttc aaccaccatt cataggtttc ttcttcgggc
     5041 accggtgcca tcgcaatccc tgccttccta tcccaatcaa cagcttcaca cagaaccctt
     5101 cgaacgaaga gaaccggtgc gttcacggag tctgatgacc cctcgcgctt gctcgccggc
     5161 gtgtgaccga atgagattga ggctcacgcg ccttcgttcc ttttgtccgc gaagcaactt
     5221 gtaactggcg cctgaaatgg gtaacagaaa ccctcgcctt ttttagctcg tcgtgccaga
     5281 actaacgcgg cgtgcgacct tggtctgttg cttctggtct ccccggttcc ctcccatcac
     5341 aactgctccc atcgaaaatc gccacgttca tccgttcgac cacagcactc gtgtgtgttt
     5401 cggacaagtg cgcataccgt ttcaccatct ggagtgtctt gtggcccaac actgcggcga
     5461 tatcgagagg cgatcccctg ttcattgcca agtacgatgc agcggaatgg cggagatcgt
     5521 ggaagcggaa gttcttcagt tctgctcgct tcaatgctga ccgccacgcc gattcgatat
     5581 cgatcgattt tgaactgcct tcaggagccg ttggcgcggg gaaaacaagc gcggagtccg
     5641 agagtcggtc tttccacaac tccgccagcg ccccgcgcgc gtgacttttc agtgggacga
     5701 tccgaacctc cgcgttcttg g
//
LOCUS       DAOSOO010000200         5662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14431_length_5662_cov_5.186374, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000200 DAOSOO010000000
VERSION     DAOSOO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14431_length_5662_cov_5.186374"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="arsM"
                     /locus_tag="PLT86_11940"
     CDS             complement(<1..736)
                     /gene="arsM"
                     /locus_tag="PLT86_11940"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOT37365.1"
                     /translation="MSELIHITRGKIRKRYAEIATSGATSCCSSSACCSGTPGEKADA
                     TQAALAAGYTLEELATLPEGANMGLSCGNPLVLASIGEGEVVLDLGSGGGLDVFLAGN
                     IVGAGGRAIGVDMTPEMVTKARNNIATYTERTGFNNVEFRLGEIEHLPVADESVDVII
                     SNCVVNLSLDKAAVFNECFRVLKPGGRLSISDIVADKPLPESMRTDDDLVCGCIGGAA
                     AVDEIRTWLENAGFQDIRVEIEEESRE"
     gene            complement(772..1104)
                     /locus_tag="PLT86_11945"
     CDS             complement(772..1104)
                     /locus_tag="PLT86_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37366.1"
                     /translation="MNALVTINAGICGFVTSAEVSSPDNQNVEFHVSSNCEKIAKLGK
                     SLKQYEPIDAYQEISPASDSVFMAIVRDNLKGCCSGCAVPSGLFKAMQVAAGLALPKD
                     ISISLSKR"
     gene            complement(1171..1620)
                     /locus_tag="PLT86_11950"
     CDS             complement(1171..1620)
                     /locus_tag="PLT86_11950"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT37367.1"
                     /translation="MKPKLKILFLCTGNSCRSQMAEGWTRYLKGDEIEPFSAGIETHG
                     LNPRAVRVMAEAGVDISGHRSKHVDELRDVAFDFVVTVCDHAHESCPMWLGGKARVVH
                     VGFDDPPRLAQNSETEEEALSHYRRVRDEIREFVETLPAALKKLTPG"
     gene            complement(1605..1970)
                     /locus_tag="PLT86_11955"
     CDS             complement(1605..1970)
                     /locus_tag="PLT86_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT37368.1"
                     /translation="MRDLLAITKALADSNRIRILAMLRAGELCVCQIVEVLGLAPSTV
                     STHLSILYGAKLVESSKRGRWVYYRLPDPRSSILVNDCIEWVIRSVENDETIEEDRVK
                     VARVISVCPEELCRTSNQS"
     gene            2116..2451
                     /locus_tag="PLT86_11960"
     CDS             2116..2451
                     /locus_tag="PLT86_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT37369.1"
                     /translation="MQMLTLNNAQAQARYEARARIIKALAHPTRLFIVDVLAQQSHCV
                     CELTAMVGADTSTVSKHLSLLKDAGLVSDEKRGTQVYYHLVAPCALRFLDCAEAVMKV
                     RLEKQLAAV"
     gene            2508..>2833
                     /locus_tag="PLT86_11965"
     CDS             2508..>2833
                     /locus_tag="PLT86_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT37370.1"
                     /translation="MNWKDEWKPLALILAIFFAAFYLPVGIPRFDNAVSEAFQLTKWY
                     AREHVVLCLIPAFFIAGAISVFVNQAAVMRYLGAKAKRTLAYGVASVSGTILAVCSCT
                     VLPLFAG"
     assembly_gap    2834..2933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2934..3680
                     /locus_tag="PLT86_11970"
     CDS             <2934..3680
                     /locus_tag="PLT86_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT37371.1"
                     /translation="PEGGRPLWQTAVYFAAMVGVLVFANWGKSDQTEAIWYAIWSVKW
                     YLTAGFAILLAVLLNRWFSIPFWQVGVASLIVLGMALAFPEEPVVTFGVGAVALSVIT
                     SLRTDESGDWFAASWGFAKQITPLLLFGVLAAGFALGRPGHEGIIPSQWVSAAVGGNG
                     VGANFFASIAGAFMYFATLTEVPIVQGLLGAGMGKGPALALLLAGPALSLPSMLVIRT
                     VVGTGKTITYVSLVVVMATISGLIYGAIFA"
     gene            3696..3929
                     /locus_tag="PLT86_11975"
     CDS             3696..3929
                     /locus_tag="PLT86_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT37372.1"
                     /translation="MKKLQILGTGCPKCKKLTEATEQAARELGIEFEIEKVTDIDKIL
                     EFGVMMTPALAVDGVVKVVGKVPTPQEIKALIQ"
     gene            3958..4377
                     /locus_tag="PLT86_11980"
     CDS             3958..4377
                     /locus_tag="PLT86_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOT37373.1"
                     /translation="MADKPCCEPVAQNDCCNGATVLIYPCSGSSDVGEISDHAGRLLT
                     REGFGKMHCLAGIGGRVPGITLSAQAAGKILAIDGCPQDCASKTLSVAGIEDFSRVRV
                     TDLGMPKGKTPVTEEHTRVVAEKGIEALRASALRATA"
     gene            4374..4988
                     /locus_tag="PLT86_11985"
     CDS             4374..4988
                     /locus_tag="PLT86_11985"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOT37374.1"
                     /translation="MNAKRWVGAGLLLFVIGSLGVMGFRSLRGGAAQAESTPRLEPTS
                     SPAPMTPPVQSGEESSVGRPPGPGRPKPAVPAPEPERVQTPAQATPESNGHLVVYYFY
                     GNRRCYSCVTIQRLTQKALEQGFPDELRRGKIEWRPTNVETPETQHFIADYRLSSQSV
                     ILSQVKNGQQVGWQNLQQVWYLLGNEEEFIAYVQDGIRAALRAL"
     gene            4988..>5662
                     /locus_tag="PLT86_11990"
     CDS             4988..>5662
                     /locus_tag="PLT86_11990"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOT37375.1"
                     /translation="MPDFGFGSTFAALAAFWLGVLSAVSPCGLATNIAAIAFIGKNVK
                     SPSSTVWSGLFYALGRITTYVVIGMVVVAGLTAVHVLSNFLQTSMNRFLGPLLVLVGM
                     VTLDLLPLRLPETGFLTRFSSNTKVQGIAGAFGIGLVFALAFCPVSAALFFGGLIPLA
                     VEKQSTLLLPFIFGLGTAIPVAAFAVVIGAGAYSLSKLFAAVTAVELWARWVTGVVFV
                     GIGIYFT"
BASE COUNT         1122 a         1589 c         1607 g         1244 t
ORIGIN      
        1 gttcgcggct ttcttcctcg atctcaacac ggatgtcctg gaatccagcg ttctccaacc
       61 atgtgcgaat ctcatccacc gcggccgccc cgccgataca gccgcacacg aggtcatcat
      121 ccgtccgcat gctctcgggc agaggtttgt ctgcaacgat gtccgaaatc gaaagccgtc
      181 cgccaggttt cagaacacgg aagcattcgt tgaatactgc agccttgtcc agcgaaagat
      241 tcactacgca gttggatatg atgacatcta cgctctcgtc ggcaacagga aggtgttcga
      301 tctcacccag ccggaactcc acgttgttga agcccgtgcg ctctgtgtac gttgcaatgt
      361 tgttccgcgc ctttgtcacc atctccgggg tcatatccac gccgatggcg cggcctcctg
      421 ccccgacgat gttgcccgca aggaacacgt caaggccccc gccgctgccg aggtcgagaa
      481 cgacctctcc ttcgccgatc gatgcgagta cgagcggatt gccgcaggat aaccccatgt
      541 tcgcaccttc cgggagtgtc gccagttctt ccagcgtgta tccggcagcc agagcggcct
      601 gcgttgcgtc agctttctcc ccgggtgttc cggaacaaca cgcagagctc gagcaacagg
      661 atgtggcgcc ggaagtcgcg atttcggcgt aacgcttgcg gatcttgccc cgcgtaatgt
      721 gtatgagttc agacacgaaa gtccctcctg gaaccaggct cccgtcgctt cctatcgttt
      781 gctcagtgag atggaaatgt ccttcggcaa ggcgagcccg gccgcaacct gcatcgcttt
      841 gaacaggccg gacgggacgg cgcatccgga acagcaccct ttcagattgt cccttactat
      901 cgccatgaac acgctgtcgg aagcggggct tatctcctgg tacgcgtcga tcggctcgta
      961 ttgttttaga gatttcccca gcttggcgat cttttcgcag ttgctcgata catggaactc
     1021 cacattctgg ttgtccgggc tggacacctc cgcagaagtg acaaagccac agattcccgc
     1081 attgatcgtc accaacgcat tcaaatcgca cctcatgtcg tgtggaaggg aaaatcacac
     1141 ggagtactca gctccgcagc tcaaattcaa tcaacccggt gtgagttttt tgagggcagc
     1201 gggtaacgtt tcgacgaact cccggatctc gtcgcgcacc cgccggtaat gactgagcgc
     1261 ttcctcttcc gtctccgaat tctgtgcgag acggggcggg tcatcgaatc ccacgtggac
     1321 cacacgcgct ttgccgccaa gccacatggg gcaggactca tgcgcgtgat cgcacacggt
     1381 tacaacaaaa tcgaacgcga catcccgaag ctcatcgacg tgtttggagc gatggcccga
     1441 aatatctacc ccggcttcgg ccatgacacg cacggcacgg gggttgagcc cgtgcgtttc
     1501 gatacccgct gaaaagggtt ctatttcgtc acccttgagg tatcgcgtcc atccttccgc
     1561 catctggctc ctgcacgaat tgcccgtaca caggaacagg attttcagct ttggtttgat
     1621 gttcggcaca gttcctccgg acagacggaa atgacgcggg ccaccttaac ccggtcttcc
     1681 tcgatggttt cgtcgttctc aacggaacgg atcacccatt cgatgcaatc gttgacaagg
     1741 atacttgaac gggggtctgg caggcggtag tacacccatc ggccgcgctt gctgctttcg
     1801 accagcttcg ccccgtacag aatggaaagg tgggtggaga cagtagatgg tgcgaggcca
     1861 agaacctcaa caatctgaca gacacagagt tcgcccgcac gcagcatcgc gagaatcctg
     1921 attcggttgc tgtccgcaag tgctttcgtg atagcgagca ggtcacgcat tgcaattcct
     1981 catttcggca tataacgaat tataccacac cgagcctgct gacgcaagag cacgaaagaa
     2041 atcaatcccc gatttggggt gcctcttgac aatccttggc gaattcgcca aatttagaaa
     2101 gtgaaaggaa cgcacatgca gatgctcacc ctgaataacg cacaggctca ggcccggtac
     2161 gaagcacgcg ccagaatcat caaggccctc gcgcacccca cgcgcctgtt catcgtagac
     2221 gtactcgcgc agcaatccca ttgcgtgtgt gaactgaccg cgatggttgg cgcggatacg
     2281 tccacggtct ccaaacacct cagtctgttg aaagacgccg gccttgtttc agacgagaaa
     2341 aggggcaccc aggtgtatta ccaccttgtg gcaccctgtg cgctccggtt tctcgactgt
     2401 gccgaagcag taatgaaagt tcgtctggag aagcaattag ccgccgtctg agtttttttg
     2461 ttgtgtttct tggttatttt gccacatagt gaaaaagaag gtccgtcatg aattggaaag
     2521 atgaatggaa acctctggct ctgatcctcg ccatcttctt cgcggcgttt tacctgcccg
     2581 ttgggatccc acgatttgat aacgcggtat cagaggcatt tcaactcaca aaatggtacg
     2641 cgcgggagca cgttgttctg tgcctgattc cggcgttctt tattgcaggg gcgatttccg
     2701 tcttcgtgaa ccaggccgcg gtgatgagat acctcggcgc gaaggcgaag agaactcttg
     2761 cgtacggagt tgccagtgtc tccggcacga tcctcgcagt atgctcatgc acggttctcc
     2821 cgttgttcgc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgaag
     2941 gcgggaggcc gctctggcag acggccgtgt atttcgccgc catggtcggc gtcctcgtgt
     3001 ttgcgaactg gggcaaatcc gaccagacag aggcgatatg gtacgccatc tggtcagtga
     3061 aatggtacct gacggcgggg tttgcgattc ttttggccgt tctgctcaat cgctggtttt
     3121 ctattccgtt ctggcaagtt ggtgtggctt cgctcatcgt cctcggaatg gcgctggctt
     3181 tccctgagga accggtggtg acattcggtg tcggtgccgt cgcattgtcc gttatcacca
     3241 gcctgcgaac cgacgaatcc ggcgactggt tcgccgcatc atggggcttc gccaagcaga
     3301 tcaccccgct tctgctgttc ggcgtgctgg ccgcggggtt cgcccttggc cggccaggtc
     3361 acgaaggcat catcccatcg cagtgggtga gcgctgcggt cggtgggaac ggtgtgggtg
     3421 cgaatttctt cgcatctatc gcgggcgcgt tcatgtattt tgctacgtta actgaagtgc
     3481 cgattgtcca gggtttgctc ggagcaggga tgggaaaggg tcccgcgctg gcgctgttgc
     3541 ttgccggacc ggctctttcg cttcccagca tgcttgtcat acgtacagtg gtaggaacag
     3601 gcaaaacaat cacgtacgtg agcctggttg tcgtgatggc gacaataagc gggctcattt
     3661 acggggccat tttcgcgtaa aaaggaaggt tcaacatgaa gaagttgcag attctgggca
     3721 cgggctgtcc caagtgcaaa aagctcaccg aggccacgga acaggccgcg cgtgaactgg
     3781 gaatcgagtt tgaaatcgaa aaggtgacgg acatcgacaa gattctggaa ttcggggtga
     3841 tgatgacgcc ggcgcttgcc gtggacggag tggtgaaggt agtcggcaaa gtgccgaccc
     3901 cgcaggaaat caaggccctg atccagtaaa cttcgagacc cgtggaaagg aacacaaatg
     3961 gcggacaaac cctgttgcga gccagttgcg cagaacgact gctgcaacgg cgcgaccgtt
     4021 ctgatctacc cgtgctccgg atcttccgat gttggcgaga tttccgatca cgccggcaga
     4081 ctgctcacga gagaaggctt cgggaaaatg cattgccttg cgggaattgg cgggcgtgta
     4141 cccggaatca ccctcagcgc acaggctgca ggcaagatcc ttgcgattga cggctgcccg
     4201 caggactgcg cgtccaaaac gctttccgtc gcaggcatcg aggacttctc ccgcgttcgt
     4261 gtcaccgatc tcggaatgcc gaaagggaaa actccggtca ctgaggaaca cacgagggtt
     4321 gtcgccgaaa agggtatcga ggcgttgcgt gcatcagcat tgagggcaac cgcatgaacg
     4381 cgaagcgctg ggttggagca gggctcctcc tgttcgtaat cgggagcctc ggggtaatgg
     4441 ggttccggag cttgcgtggt ggtgctgctc aggctgaatc aactccccgt ctggagccga
     4501 catcgtcacc agcgccaatg acgcctcctg tgcaatccgg cgaagaatcg agtgttggcc
     4561 ggccgccggg acctgggcgg ccgaaaccgg ctgtccctgc gcctgagcca gaacgggtcc
     4621 agacgcccgc acaggctaca ccggagagca acggccacct tgtcgtctac tacttctacg
     4681 gaaaccggcg atgctacagc tgcgtcacca ttcagaggct cacccagaaa gctctggaac
     4741 aaggctttcc ggacgaattg agacggggaa aaatcgagtg gaggccgacc aacgtcgaga
     4801 cgcctgaaac gcagcacttc atcgcggatt accgcctttc atcgcaatca gtgattctgt
     4861 cgcaggtcaa gaacggacaa caggtcgggt ggcagaacct tcaacaggtc tggtaccttc
     4921 tgggcaacga ggaggaattc atcgcgtacg ttcaggacgg catccgggcc gccttgaggg
     4981 cgttgtaatg cccgacttcg gtttcggaag cacgttcgcg gcgcttgcgg cattctggtt
     5041 gggtgtgctt tctgccgtta gcccgtgcgg acttgcaacg aacatcgccg cgatcgcgtt
     5101 catcgggaag aatgtgaaga gcccctccag cactgtctgg agcgggcttt tctatgccct
     5161 tggccgtatc accacgtacg tggtgatagg catggttgtg gttgccggcc tgaccgcggt
     5221 gcacgttctt tcgaatttcc tccagacttc catgaaccgt ttcctcgggc ccctgcttgt
     5281 tctcgtcggg atggtcaccc tcgatcttct tcccctcaga ttaccggaga caggctttct
     5341 cacgagattc agcagcaaca cgaaggttca gggaatcgcc ggtgcgttcg gcatcggcct
     5401 cgtgttcgca ctcgcctttt gtccggtttc cgcagcgttg ttttttggag gattgatccc
     5461 tctcgccgtg gagaagcagt ccacgttgct gcttccgttc atcttcggac tcgggactgc
     5521 aattccggtg gcggcgttcg cggtggttat cggcgcgggc gcttactcac tttccaaact
     5581 tttcgccgct gtcaccgcgg tggagttgtg ggcaaggtgg gtaaccgggg tggtttttgt
     5641 cgggatcgga atctacttca ca
//
LOCUS       DAOSOO010000201         5587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14657_length_5587_cov_5.039769, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000201 DAOSOO010000000
VERSION     DAOSOO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14657_length_5587_cov_5.039769"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLT86_11995"
     CDS             complement(<1..282)
                     /locus_tag="PLT86_11995"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT37376.1"
                     /translation="MPLSVDVALPLAVDAVFTYSVPEELSDGALVGMRAVVPFRRNLL
                     TGMIVAVHENGAEPAQRVLRPVADFPDPSPSLDPTMLSLTRWIADYYLSS"
     gene            complement(293..1153)
                     /locus_tag="PLT86_12000"
     CDS             complement(293..1153)
                     /locus_tag="PLT86_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOT37377.1"
                     /translation="MSDDTVETSDTSEARISLADRFRRSREERGLSLQDVADRSRISV
                     TILQHYESGDFSVVEPAFVRGHLRTYARIVGVEEREIDSVFPPSGLSVSGLTALEDDE
                     AQEALFIEKRPALPAIPWKTAAWFGLGAALLVALWLWHPWHSPENSFAESPPEVESAV
                     APAQKENAEITAPAENDTLSDEPAVRLATPPELAKARVPGVVRTMTIEATDSVWVLIA
                     RKDGMVLFDGILVPGQSKQSDVEDTLRVTLGRYWAARLLLNRQAVPTPRQEARNMTFF
                     LCTPSGIVPP"
     gene            complement(1156..1782)
                     /locus_tag="PLT86_12005"
     CDS             complement(1156..1782)
                     /locus_tag="PLT86_12005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT37378.1"
                     /translation="MTGPNLSPPPWFVSEQPHPLILASASPRRAELLNQVGIRFLQAP
                     VEVDETWPKDLLPGEAVRSLARRKASVAATRHPGAVILAADTAVFLDSQPLGKPADAT
                     EAYEMLRRLSGRKHEVITGITIVDTISHLEISDSVLTEVWMREANDYEIAVYAKSSEP
                     LDKAGAYGIQGMAAGFVTKICGCYSNVVGLPVSRVVAMLQLIVARRKE"
     gene            2127..2879
                     /locus_tag="PLT86_12010"
     CDS             2127..2879
                     /locus_tag="PLT86_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT37379.1"
                     /translation="MQIDEMVRIPATSFLMGDDHSDADVRPAHRVDVSAFFIDRREVT
                     CEEYRRFLLANPEWRKGRVSPSLADRNYLRDWDDLSYPRGKAAHPVTWVSWHAANAYA
                     KWRGARLPTEAEWEATARGATGRIYPWGNEDPENSEMPRANYASARGRFGRHGGTEPV
                     GRYPAGATPEGVLDMAGNVWEWVADWHDPDYYSESPRVNPTGPSNGSYRVVRGGSWAV
                     PSPWLRSYARLRAFPAKTSDQVGFRCARTSSP"
     gene            2992..4389
                     /locus_tag="PLT86_12015"
     CDS             2992..4389
                     /locus_tag="PLT86_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOT37380.1"
                     /translation="MKRGIILFVCVVISVGTAWSANWNTRRNPKNVIILVGDGMGFNQ
                     VEAGSLFSGRQVFERFPVRYGCTTFSASGNGYQPDSAWASFDYLKKRATDSGAAATAL
                     ASGTKTTNGAIGVGPDGDPVPNILEVAEDVGKATGVVSSVGISHATPAGFVAHSRSRG
                     SLESIAQEMILRSRVDVIMGAGNPWFDDNGNALAEPKNFRWVGGEQVWKGLLLSGARF
                     DLDTNGTYETTVADADGDGVPDRWELVQTRRAFRQLAAGPTPKRVLGLPQIHETLQQK
                     RTDQRGSNLRQDYANDEPYAVPLNEKVPTLAEMTRAAINVLDNDPDGFVLMVEGGAID
                     WAGHANLSGRLIEEVAGFTEAIEAVVAWVDSASSWDETLVVVTADHETGYLWGPGTGF
                     GADGAIQWNPLQGQGLRQMPAMQWNSTGHTNSLVPFFAKGRCSERFARFVDGTDPRYG
                     QFVDNTAIFRMLTED"
     gene            complement(4458..>5587)
                     /gene="der"
                     /locus_tag="PLT86_12020"
     CDS             complement(4458..>5587)
                     /gene="der"
                     /locus_tag="PLT86_12020"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT37381.1"
                     /translation="MEAHILDQVQIAMEEASLVLFLTDAQTGVTDLDAEVARVLRKKE
                     RQTLLVVNKVDNPEADVEVHAFHALGMGSPVPVSAMTGRHVGDMLDQLVKRLPPEAEG
                     EEPEGGIAVAFVGRPNVGKSSLVNALIGSPRVVVDDVPGTTRDATDTFFQHEGRYYTL
                     VDTAGLRRQKNVWKTRDAIEHFSVLRTLKAIERCDVAVLVIEAMDHLVKQDSDILAQI
                     VDAGKAVVIAANKWDAVPGKTSETAGAFMKELWVRNPFTEHVPVSLISAATGQRVEKV
                     FSDVLTAYEQWTRRIPTNTLNEWLRELVKKNPPPISRRGVPRLSYVTQVGVRPPTFVF
                     FVNNPDAIGTAGERYLERCLREEYGFAGTPLRLLFRKKSRP"
BASE COUNT         1168 a         1580 c         1696 g         1143 t
ORIGIN      
        1 gctcgacaga tagtaatcgg ctatccagcg agtgagagaa agcatggtgg ggtcgaggga
       61 cggagaaggg tcggggaaat cagctacggg tctcaggaca cgctgggcgg gctcagcacc
      121 gttctcgtgg acggcaacaa tcatcccggt aaggaggttt cgccggaacg ggacaacggc
      181 acgcatgccc acgagtgcgc cgtctgatag ctcctcagga acggaatagg tgaatacggc
      241 gtcgaccgcc aagggcagcg caacgtcaac cgagagaggc atcacctgtt tctcacggag
      301 gcacgattcc cgatggagta cacaaaaaga aggtcatgtt gcgagcctcc tgccgcgggg
      361 taggaacggc ctgccgattc agcagaagcc tcgccgccca gtagcgtcca agggtaaccc
      421 gcaacgtgtc ttccacatca ctctgtttac tttggccggg cacgagaatc ccgtcgaaaa
      481 gcaccatgcc gtcttttcgt gcgatgagca cccaaacgct gtcggtggcc tctatggtca
      541 tcgttctcac cacgccggga accctcgctt tcgccaactc cggtggcgtt gcaagtcgaa
      601 ctgcgggttc atctgagagc gtgtcgtttt ctgccggcgc ggtgatctcg gcgttttcct
      661 tctgggctgg agcaaccgcg ctctctactt cgggagggga ttccgcgaat gaattttccg
      721 gactatgcca ggggtgccag agccagagtg cgacaagcag ggctgcaccc aatccaaacc
      781 aggccgctgt cttccatggt attgcgggaa gcgcaggtcg tttctcaatg aagagggctt
      841 cctgcgcttc atcgtcttcc agggccgtca ggccggaaac ggagagcccg gacggcggaa
      901 agactgagtc tatttctcgc tcttcaacac cgacaatccg ggcgtaggtc cgcaggtgcc
      961 cccgaacgaa cgccggctcg acgacggaga aatcccccga ttcatagtgc tggagaatcg
     1021 taacactgat gcgcgaacga tccgccacat cctgcagact cagcccacgt tcttcgcgcg
     1081 atctacgaaa gcggtctgca agactgattc gcgcttcgga ggtgtctgag gtttcaacgg
     1141 tgtcgtcaga catccctatt ccttccgtcg agcgacaatg agctgcagca tggccactac
     1201 acggcttacg ggtaaaccca cgacgttgga atagcaaccg catatcttcg tcacaaaccc
     1261 cgccgccatg ccttgaatgc cgtatgcgcc agccttatcc agaggctcgg acgatttcgc
     1321 atacacggcg atctcgtaat cgttcgcttc gcgcatccac acttcggtga ggacagaatc
     1381 agagatctcc aaatgcgaaa tggtgtctac tatggtgatg cccgtgatga cctcatgttt
     1441 tcggcccgac aaccgccgca gcatctcata cgcttccgtc gcatctgccg gtttgccgag
     1501 cggttgcgag tccaggaaaa cggcagtatc cgcggcaagg attactgctc cgggatgccg
     1561 cgtggcggca acactcgctt tccttctggc cagcgatctg acagcttcac cgggcaacag
     1621 gtccttcggc caggtttcat caacctccac aggcgcctgc agaaagcgga ttccgacctg
     1681 attgagcaac tcggcgcggc gcggagacgc agaggccaga atcagagggt gaggctgttc
     1741 cgacacaaac catggcggtg gggaaaggtt aggaccagtc attcgtaatt tccgataccg
     1801 cgatgcttgc acgtgcgggc ccggcgcaca cacgaatccg tgcgcggcgg tttccgaagg
     1861 tgtctgccgc cgttcaccat gaactggaac ggcgcgtaca tttgtctgca aacggaccgg
     1921 tcggacccgg tgtggttgat tgccttgtcc cgatccagtc gttcaaaaag atacgaaaca
     1981 ggcgtgtcac tggcacgaaa atggcgcgca agggaagaga ggtgatcacg ctgagaaggc
     2041 atggggtttt ttcgctgtgg tgtatggcgt tgggttgggt actgccggcc aatttggcag
     2101 gtgaagccct tcccgcgcgg aacgacttgc agattgatga aatggtgcgt attccggcca
     2161 cttcgttttt gatgggagat gatcattccg acgccgacgt acgtccggca catcgcgtcg
     2221 acgtctcggc gttcttcata gatcggcggg aagttacgtg cgaagagtac cgccggtttc
     2281 ttctcgcgaa cccagagtgg cgcaaggggc gagtctcacc ctctctggcg gacaggaact
     2341 atcttcgaga ctgggacgat ctatcatacc cccggggaaa ggctgcgcac cctgtgacct
     2401 gggtcagctg gcatgcggcc aacgcgtatg cgaagtggcg cggggcgcgt cttccaactg
     2461 aagcggagtg ggaagcaacc gcgcgcgggg caacaggacg aatctacccg tggggaaatg
     2521 aagacccgga gaactcagaa atgccgcgcg cgaattacgc gtctgcacgc ggcagatttg
     2581 gccggcacgg tgggacggaa cccgttggcc gttatcccgc tggcgcaacg ccggaagggg
     2641 tacttgacat ggcgggcaac gtgtgggaat gggtggcgga ctggcacgat ccggattact
     2701 actccgaaag ccctcgagtg aatcctaccg gtccatcaaa cggaagctat cgtgtcgtcc
     2761 gcggcgggtc ttgggcagtt ccctcgccct ggctccgttc gtacgccagg ctgagggcat
     2821 tccccgcaaa aacaagcgat caagtgggtt tccgttgcgc cagaacctct tcgccctgat
     2881 cttttgtatc atgcgcagtt gtgagcctcg cgtgatccct cgcgcaacca gaatgcattt
     2941 ggggattgtt cgcaggttct ccttcattgg ccaaagccga aaggatcagt cgtgaaaagg
     3001 ggaattattc tgttcgtttg tgtcgtgatt tccgtgggaa ctgcatggtc cgcgaactgg
     3061 aacacgcggc gcaatccgaa aaacgtcatc attcttgtcg gtgacggcat gggtttcaac
     3121 caggttgagg cgggttctct tttttcgggc agacaagttt tcgaacgttt cccggtgcga
     3181 tatggatgca ccacgttttc cgcttccggg aacggatatc aacccgattc agcatgggct
     3241 tcgttcgatt acttgaaaaa aagagcaaca gattccgggg ctgcggcgac cgcgctcgcg
     3301 tccggaacga aaacaaccaa cggcgctata ggagtcgggc ctgacggcga tcctgttccg
     3361 aatattctcg aggttgccga ggatgtcgga aaggcgacgg gtgtggtgag ttcggtgggc
     3421 atatcccacg cgactccggc cgggtttgtg gcccacagcc ggagcagggg tagccttgaa
     3481 tccatcgccc aggagatgat ccttagaagc cgggtggacg tgattatggg ggcgggcaac
     3541 ccatggttcg acgacaacgg gaatgcgctg gcagagccga aaaacttccg gtgggtcgga
     3601 ggagaacagg tgtggaaggg gcttttgtta tccggcgcgc gcttcgacct ggacacgaac
     3661 ggaacgtacg agacaacggt agcagatgcc gacggagacg gtgtcccgga tagatgggaa
     3721 ctcgtccaga cgcggcgggc atttcggcaa ctggcggcag gcccgacacc gaaacgggtc
     3781 ctcggcttgc cacaaataca tgaaacgttg cagcagaaac gcacggatca gcgtggatcg
     3841 aacctcaggc aggactacgc gaacgacgag ccttatgcgg tgcccttgaa tgaaaaggtt
     3901 cccacccttg cggaaatgac gcgagctgcg atcaacgttc ttgataacga tcccgatggt
     3961 tttgtgttga tggttgaggg tggcgcaatt gactgggccg gacacgccaa cctcagcgga
     4021 cggctcatcg aggaggtggc aggcttcacg gaagcgatcg aagcagtagt agcatgggta
     4081 gattcggcaa gcagctggga cgagaccttg gtggttgtca ctgccgacca cgagacggga
     4141 tacctgtggg gaccgggaac agggtttggg gccgacggag caattcagtg gaacccattg
     4201 caaggccagg gactgcgcca gatgcccgcg atgcaatgga acagcaccgg ccacacgaac
     4261 agcctcgttc cgttcttcgc aaaagggaga tgcagcgaac gttttgcccg gtttgttgat
     4321 ggaaccgatc cgcgttacgg gcagttcgtg gacaataccg cgatcttccg catgttgaca
     4381 gaggattagc cgggagttct gcacgtatcc gcgtttggtg ggcaatgcca gagaccctga
     4441 gaccggtgca ccttgtctca gggtctcgac tttttcctga acagaagcct cagcggagta
     4501 cccgcaaagc cgtattcttc ccgcagacat cgttcgagat atcgttcccc cgcggtaccg
     4561 atggcgtccg ggttgttcac aaaaaacacg aaggtcgggg gccgcacacc tacctgggtc
     4621 acatacgaaa ggcgcgggac gcctctccgg gaaatgggtg gagggttctt tttgacgagt
     4681 tccctgagcc attcgttcag cgtgttcgtg ggtatccttc gagtccattg ctcgtacgca
     4741 gtgaggacat cggagaacac cttctcgacg cgctgcccgg tagcggcact gatcagagag
     4801 accggaacat gttcggtgaa cgggttccgc acccatagct ctttcatgaa tgcaccggcg
     4861 gtttctgatg tcttgcccgg taccgcatcc catttgttcg cggcgatgac gaccgctttc
     4921 ccggcatcca cgatctgagc gagtatatcg ctgtcctgtt tgacgaggtg atccatcgct
     4981 tcgatcacca gtaccgccac gtcgcagcgc tcgatcgctt tgagagtgcg caacacgctg
     5041 aaatgttcta tggcgtcacg ggttttccag acgttcttct gccgccgcaa cccggcagtg
     5101 tcgaccagcg tgtagtatct gccctcgtgc tgaaagaatg tgtcggtcgc gtctctcgtg
     5161 gtgcccggga cgtcgtccac aaccactctg ggagacccga tcagcgcatt cacgagagac
     5221 gatttgccca cgttcggccg gcccacaaac gcaactgcga tcccgccttc cggctcctca
     5281 ccttctgcct ccggcggcaa tcgtttcacc aactgatcca gcatgtcccc gacatgccgg
     5341 ccggtcatgg cggagacagg cacagggctt cccatcccga gtgcgtggaa ggcgtggact
     5401 tcgacatcgg cctccggatt gtcaactttg ttgactacca gcagggtctg ccgctctttt
     5461 ttcctcaaaa cgcgcgcgac ttctgcgtcc agatcggtga ctccggtctg agcgtcagtg
     5521 agaaaaagca ccagagaagc ttcctccatc gcgatctgga cctggtcgag gatgtgcgct
     5581 tccatcg
//
LOCUS       DAOSOO010000202         5528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14834_length_5528_cov_6.083684, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000202 DAOSOO010000000
VERSION     DAOSOO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14834_length_5528_cov_6.083684"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="PLT86_12025"
     CDS             complement(<1..1476)
                     /locus_tag="PLT86_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37382.1"
                     /translation="MEDRLTRILELLKANELPVTWKAIIGDARGAESPEYDDSAWDEG
                     WRHLATKRWATAWLRASVPASPLLSEPISGRFVIGGHLTVWVNGMKLGEGFEPTISLP
                     VPALMANGLKIAVKSEEGGLSSEMLKLILFQSPRLRQVKTLRRSLDFVMQWRAAQPGN
                     AAAINDVLARYVAAVDPDAAPEVFLAQVVEANELLRQLDSLAKQYTVHIVPHSHVDLS
                     WGWDFSETKRIARALFDQALRIMEVDPAYTFAQDQPPMYVHLEDSATEKAIARRVKEG
                     RWDIPGATFSEPESFMPGGESWVRHLLYTKRYFKQRFGKEISLHWAPDNFSGHANTLP
                     QIWKLCGINAFVFGNWYQAPHGGQFLWEGLDGTRVFAHYMTSHYDSAQMIEQDKVIRN
                     VCEHMKATTIPACMLLDGDDLTPPWPGSPQGIENLRAFAAFPKIEFSTPHRFFSSVDT
                     AADGLRVVQGEFISTTDHRHNNVGAYSTFAEVKLRNRRSEWG"
     gene            complement(1663..3207)
                     /locus_tag="PLT86_12030"
     CDS             complement(1663..3207)
                     /locus_tag="PLT86_12030"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT37383.1"
                     /translation="MKGRRVLWAVSFIGVGTFIGLILASGSLFTHTPFSPFEAHAQTR
                     LFAPPSSPRTPPPPSTATAMALGEAFNWAAERAIPAVVMIKSETTVKASQNVIPNLPD
                     FFPRDLFPQIPDQSFRREGLGSGVIVSSDGYIVTNNHVIQGADKLTVVIGDDGVETSA
                     RVVGTDPRTDVAVIKVDLTNLPTLELGNSDSIKIGHWVVAIGSPFGEALRHTVTTGIV
                     SALGRNIGIVDSDNRSGYTGFESFIQTDAAINPGNSGGALVDLRGRLIGINTAISSTS
                     GSNSGVGFAIPINLVRDIARQLVENGRVSRGYLGVQIQQITPEIQNSLNLPDRHGALV
                     GDVTSGGPAARAGIQRGDVIRTINGTAVKDMNDLRLRIASTPPGQQVRLGLWREGRER
                     TVTATLDELPAEFQLPSERTSSSSRTGAVSRLGLTVTNASEATGRRGGEAQTGVLVTA
                     VDPNSPAASRGLRQGDIIQEVNLQPVANVQEFERKIAAVKAGDSVLLLVKRDNSTLFI
                     GVTIPN"
     gene            3395..3470
                     /locus_tag="PLT86_12035"
     tRNA            3395..3470
                     /locus_tag="PLT86_12035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3428..3430,aa:Gly,seq:ccc)
     gene            3623..5215
                     /locus_tag="PLT86_12040"
     CDS             3623..5215
                     /locus_tag="PLT86_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT37384.1"
                     /translation="MPASRHTWLRAYHTDNPFDRSLTRPLTIVASEDIEVAWQPPGSA
                     EPKAVLSCSRYDFIFPYRTIENEERVGRGRIFLPPSCETSPKPRSVPLVLSIHYEGDQ
                     GWASGFLAQGWACMTPIALGPDHGGNLAGEGMDHTLAMAQLVRRLGFVDLQRIGWTGG
                     SAGGFQCLMAMESLWPVACANADVPVVDLYYNIRYLGTMERYNRGITDPAALVIPVIH
                     AVQSIVEKTEGYLRDDVEAYWRHSVPVGAALIRSPLIIHSATGDLLCPVAQVGDDFYR
                     PPKRSDFPPGWNMEYRKYANPLALDKPLIEWFSPEHVEQVTVAIPESAPRVDRIPVPP
                     NRPAPPPPPPPYTLVKPFSRTNLITVLIQDEGNPTPLCAHSKYAVWMDPLPCFQYHFA
                     RGYVPAEHLTPEVLTRVLGRFSKAVPQNATLPPIRRLHEHFDRWEALLALETFAGTPP
                     REENVSTLRRLYSELPPVARALDVQQDGIVAQFAENPVAGLLFHQAVELRKTGETSEA
                     EAKESHLKREYGSSAFAKLRTE"
     gene            5227..>5528
                     /locus_tag="PLT86_12045"
     CDS             5227..>5528
                     /locus_tag="PLT86_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT37385.1"
                     /translation="MDRVQGSPRAKATPRDAEPAVEIRELVKHYGATPVLRGINLTIA
                     AGTSVALFGPNGAGKSTLLNLIATLARPTSGTIRVCGYDSVKEGPRARGCAGMLSHA"
BASE COUNT         1031 a         1781 c         1594 g         1122 t
ORIGIN      
        1 cccccattcc gaacgacggt tgcgcaactt cacctctgcg aaggtgctgt acgctcccac
       61 gttgttgtgt cggtggtccg ttgtgctgat aaactcgccc tgcaccacgc gcaagccgtc
      121 cgcggcggta tcaacggaag aaaagaaccg gtgcggcgtg gaaaactcga ttttcggaaa
      181 tgccgcgaac gcgcgcagat tctcaatacc ctgcgggctt cccggccacg gcggagtaag
      241 gtcgtccccg tccagcagca tgcacgccgg aatggtcgtc gccttcatgt gctcgcacac
      301 gttgcggatg actttgtcct gttcgatcat ctgcgcggaa tcatagtggc tggtcatgta
      361 gtgcgcgaac acgcgggtcc cgtccaggcc ctcccacaga aactgccccc cgtgcggcgc
      421 ctgataccag ttcccgaaca cgaacgcgtt gattccgcac agcttccaga tctgcggcag
      481 agtgttggcg tggccggaga agttgtcggg tgcccagtgc agcgaaatct ccttgccaaa
      541 gcgctgtttg aagtaccgtt tcgtgtacag caggtgccgc acccagcttt cgccccccgg
      601 catgaagctt tccggttcgc tgaacgttgc tccggggatg tcccaccgac cttctttcac
      661 gcgacgggca atcgcctttt cggtggccga atcctccaga tgcacgtaca tcggtggctg
      721 atcctgcgcg aacgtgtagg cagggtcgac ctccatgatt cgcagcgcct gatcaaacag
      781 cgcgcgcgcg attcgctttg tctcagagaa atcccatccc caggagagat ccacgtgcga
      841 gtgcgggacg atgtgcactg tgtactgctt cgccagtgag tccagttgcc tgagcagctc
      901 gttcgcctcc acgacttgcg ccaaaaacac ctcgggcgca gcatcggggt ccaccgccgc
      961 aacgtaccgc gccagaacat cgttgatcgc cgccgcgttc ccgggttgcg ccgcgcgcca
     1021 ttgcatcacg aaatcaaggc tgcgtcgaag cgttttcacc tgccgcaacc ggggtgattg
     1081 gaacaggatc agcttcagca tttcgctcga aagcccgccc tcctccgatt tcacggcaat
     1141 cttgagcccg ttcgccatga gagcaggcac cgggaggctg atcgttggct cgaacccctc
     1201 gccaagcttc attccgttca cccagaccgt caggtgccct ccgattacga atcttccgct
     1261 gattggctcg gaaagcaggg gagacgcggg aacggaagcc cgcaaccacg cggtggccca
     1321 gcgtttcgtt gccagatgcc gccacccctc gtcccacgcc gaatcatcgt attccggcga
     1381 ttccgcgccg cgtgcgtctc cgatgatcgc tttccacgtc accggcagtt catttgcctt
     1441 gagcagttcc agaattcggg taaggcggtc ttccatgtgg gttctccgga aattcggggt
     1501 ctgtgcgggt ggccaaatcg ttgcagacgt aacataaatg cgcgatctct gcggaacaac
     1561 gctcagcggg cgagacgacg gcgaaccacc ggaacaaaaa aggagcgggc gcgtttgcgc
     1621 gcccgctccc gcctgtccga aagcatcgtt ctctacgggt tttcagttcg gtattgtcac
     1681 gccgatgaac agcgtgctgt tgtcccgttt gacgaggagc agcaccgagt cccccgcttt
     1741 caccgcggcg atcttacgct cgaattcctg cacgttcgcc acgggctgca gattgacctc
     1801 ctgaatgatg tccccctggc gcaggccacg gctggccgcg gggctgttcg ggtccaccgc
     1861 cgtcacgaga acaccagtct gcgcttctcc gccgcgccgc cctgtcgctt ccgatgcgtt
     1921 tgtcacggtg agccccagtc tggacaccgc tccggtgcgt gatgacgagc tggtccgctc
     1981 gctggggagc tggaactccg cgggcaattc atcaagcgtc gcggttacag tccgctctct
     2041 gccttcccgc cacaacccga gtcggacctg ttggccggga ggcgtgctgg cgatgcggag
     2101 ccggagatcg ttcatgtctt tcactgctgt cccgttgatc gttctgatta cgtcgccgcg
     2161 ctgaattccg gcgcgcgcag cggggcctcc ggaagtaaca tcacccacca gtgcgccgtg
     2221 ccggtccggc agattgaggc tgttctgaat ctctggcgtg atctgctgaa tctggacgcc
     2281 cagatatcca cgcgaaacac ggccgttttc caccagctgc cgggcaatat cccgaaccag
     2341 attgatcggt atggcaaacc ccacgccgga attgctgccg ctggtggaac tgattgccgt
     2401 attgatcccg atcaaccgcc cgcgcagatc caccagcgcg ccgccggagt tgcccggatt
     2461 gattgccgca tcggtctgga tgaagctttc gaaaccggta tacccgctgc gattgtcact
     2521 gtccacgatc ccgatatttc gccccagcgc gctgacgatt ccggtggtca ccgtatgtcg
     2581 cagggcttcc ccgaacggac tcccaatagc caccacccag tgcccgattt tgatgctgtc
     2641 gctgtttccc aactccagcg tcgggaggtt cgtcaaatca accttgatca ccgcaacatc
     2701 cgtccgtggg tcggttccaa caacccgggc gctcgtttct actccgtcgt ctccgatgac
     2761 cactgtcagc ttgtccgcgc cctgaatcac gtggttgttg gtgacgatgt acccgtccga
     2821 actgacgatc acgccgctgc cgagcccttc acggcgaaaa ctctggtccg gtatctgcgg
     2881 gaaaagatcg cgcgggaaaa agtccggcag gttcggaatg acgttctggg aagctttgac
     2941 ggtcgtttcg ctcttgatca ttacgaccgc cggaatcgcc cgttcggcgg cccagttgaa
     3001 cgcctcaccc agtgccatcg ccgttgccgt ggaaggtggt ggcggcgtgc gagggcttga
     3061 tggcggcgcg aataaccgcg tctgagcgtg cgcttcaaat ggagaaaacg gcgtgtgagt
     3121 aaaaagtgac cccgaagcaa ggatcaatcc gatgaacgtg cctacaccga tgaacgaaac
     3181 agcccagagg actcttcgcc ccttcatggt ggtatccttc ctttttgcga aaaaccaggg
     3241 aacgtgagat ggttcactca ctactaagtg tacgaccgga acacgcgatt caaaatgtgg
     3301 aacgcgcgcc ccgggggcgc ggttccttga acaccccgcc gacgtgcgtt cagcgcttga
     3361 cgattcttcg cgcaaccgct atactaggta gtacgcgagc gtaactcagc tggtagagtt
     3421 ccagcttccc aagctggctg tcgcgggttc gaatcccgtc gctcgctcca ttcgttccat
     3481 tccgcatcgg atcagcgatg cgtttgcata cgccgccgcc tcgaaggagc cggcggtttt
     3541 tttgtgaatt gtcccgaccc cgccgctgcg ctacattcca taggcaacac ctcttctttc
     3601 ggcacactcc gtggaggtat tcatgccggc ttcacgccac acctggttac gcgcgtatca
     3661 cacggataac cccttcgacc gatcgctgac gcgccccctc accatcgtcg cgagtgagga
     3721 tatcgaagta gcgtggcagc cgcccggcag cgcggaaccg aaggcggttc tttcgtgcag
     3781 ccgctatgat ttcatcttcc cctaccggac catcgaaaac gaagaacgcg tggggcgtgg
     3841 ccgcattttt ctcccgccat cctgcgaaac ttctccgaag cctcgcagcg tgccgcttgt
     3901 gctgagcatt cattacgagg gcgatcaggg atgggcgtcg ggtttcctcg cccagggatg
     3961 ggcctgtatg actcccatag ccctgggacc agaccacgga ggcaacctcg ccggcgaagg
     4021 aatggaccac acgctcgcca tggcgcagct cgtgcgccgt ctcgggtttg tggacctcca
     4081 gcgaatcggg tggacggggg ggtctgcggg cggcttccag tgcctcatgg cgatggaatc
     4141 gctgtggccg gtggcgtgcg caaacgccga cgttccagtt gttgacctct attacaacat
     4201 caggtatctc ggcacgatgg agcggtacaa ccgcggcata acggaccccg ccgcgcttgt
     4261 gataccggtc attcacgcgg ttcagtccat cgtcgaaaaa acggaaggat atctgaggga
     4321 cgacgtggag gcgtactggc ggcattccgt accggtcggt gcggcactca tcaggtcccc
     4381 gctgatcatc cacagtgcga ccggtgacct cctttgtccg gttgcgcagg tcggcgacga
     4441 cttctaccgc ccgccgaagc ggagcgactt cccaccgggc tggaacatgg aatatcgcaa
     4501 gtatgcgaat ccactcgcgc tggacaagcc gctgatcgaa tggttttcgc cggaacacgt
     4561 ggaacaggtt accgtggcaa tcccggaatc cgccccacgt gtggaccgca tccccgtccc
     4621 acccaaccgg ccggcgcctc cgcccccgcc accgccttac acactcgtga agccgttcag
     4681 ccgaaccaac ctcattaccg tgttgatcca ggatgaaggc aacccgactc ccctgtgcgc
     4741 gcattcgaag tacgccgtct ggatggaccc gctgccctgt ttccagtatc actttgcgcg
     4801 gggatacgtt cctgcggagc atctcactcc cgaagtgctg acaagggttc tgggtcgttt
     4861 ctcaaaggcc gtgccccaaa atgcgacact tccgcccatt cgccgtttgc acgagcattt
     4921 cgaccgctgg gaggcgcttc tcgcgctgga aacgttcgcc ggaaccccgc ctcgcgagga
     4981 gaatgtcagc actctccgca ggctgtattc ggagcttccg ccggttgcga gggctctgga
     5041 cgtgcaacag gacggcattg tcgctcagtt tgccgaaaac ccggtcgcag gcctcctttt
     5101 ccatcaggcg gttgagttgc gaaaaactgg cgaaacctcc gaagcagaag cgaaagaatc
     5161 ccacctcaaa agggagtacg gttcttccgc cttcgcaaaa ctccggacgg agtaagggtt
     5221 tctccggtgg accgggtgca ggggtctccc cgggcgaaag ccacgccgcg agatgcggaa
     5281 ccggcggtgg agatccggga gctcgtaaag cactacggcg cgaccccggt tcttcgagga
     5341 atcaatctca ccatcgccgc cggtacgtcc gtcgccctgt tcggcccgaa cggcgcaggg
     5401 aaatcaaccc tcctcaatct catagccacc ctcgcccgcc ctacgtctgg cacgattcga
     5461 gtgtgcggat acgattcggt gaaggagggt ccgcgcgctc gcggatgtgc aggcatgctc
     5521 tcccatgc
//
LOCUS       DAOSOO010000203         5526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14844_length_5526_cov_5.790349, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000203 DAOSOO010000000
VERSION     DAOSOO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14844_length_5526_cov_5.790349"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLT86_12050"
     CDS             <1..721
                     /locus_tag="PLT86_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37386.1"
                     /translation="AVRGLALGGDTGGAKAIVPGVASIETARYAHEELNSIERTAVAY
                     VNNPVRLGMEEAARLANLDISVNIIMNGDRQVAGLFAGDVVGAHRAAVRVAHERYNTT
                     LAQNADVVVSNAYPQSVEASKELKYCRMSLRDGGTAVLIQDTPAGQRKIHYLGWSGDL
                     ERRALRRRSRGLPVPQAAQVIVFNRWPCALDEMDYSPEVYHAKTWDEVVARLTRIHGE
                     GTKVAVYPAAALAHEPMPLRM"
     gene            complement(817..1887)
                     /locus_tag="PLT86_12055"
     CDS             complement(817..1887)
                     /locus_tag="PLT86_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT37387.1"
                     /translation="MREESLSFLKALLEAPSPSGYEQPAQRIWRDYVSSFADTVTSDR
                     HGNAIAAINPGGKPRVMLAGHCDELGFMINYINDQGFLNFLAIGGHDVAIVPGRRVTI
                     HTANGPVKGVIGKRAIHLMTQQERDDPKPKIEGYWIDIAVKSKDEAKELVAIGDPVTY
                     NVAFEELRNGIAVSRAFDDKAGAFVVAEALRLIAENRAGLKAAVYAVSTVQEEVGLRG
                     AQTSAFGIDPDVGIAVDVTHATDSPDMDKRKAGEVNLGGGPAISRGANISPIVFDRLV
                     DAAKKNEIAYQVEGAPRGTGTDANVIQLTRSGVATGLVSLPLRYMHTTVETIALSDME
                     QSAQMLATFCADLTPETSLIPQ"
     gene            2281..2859
                     /gene="pyrR"
                     /locus_tag="PLT86_12060"
     CDS             2281..2859
                     /gene="pyrR"
                     /locus_tag="PLT86_12060"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT37388.1"
                     /translation="MPETHEQTKSSGSPGPVVMTAAQMSRALNRMVHEILERNGGAEN
                     LALVGVRTRGYPLALRLASLIERVEGKRPPTGAVDVTFFRDDFRLRAKAPQEFTDIPF
                     AVDNKNIVLVDDVFFTGRTTRAAMETVMEFGRPRTIQLAVLVDRNHRELPIHPDYVAK
                     RVETALDEVIRVRLKEIDGEDQVLLVRGGVTP"
     gene            2856..3809
                     /locus_tag="PLT86_12065"
     CDS             2856..3809
                     /locus_tag="PLT86_12065"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT37389.1"
                     /translation="MTKWTRKHLLGLADLTREEIGQILDSADQFVSVLDRPMRSVPTL
                     RGWTVVNLFFEDSTRTRISFELAEKRLGADIVNFSVSTSSVKKGETLRDTARNIEAMR
                     VDAVVIRHRSVGAPHFLSRHIQGSVINAGDGSHEHPTQGLLDLLTIRRHFGHIDGLRV
                     AIVGDIRHSRVARSDVWGFTTMGAEVVLCGPPTLLPKHTDNFPAKVTTRLEEALEGAD
                     VVYALRIQRERQAAGLFPSIREYSAQYGISSERMERAKPTAVLLHPGPINRGWELTDE
                     VTDGPRSLILEQVTHGVAIRMAVLYLTAGRDPAQLDLAPKP"
     gene            3870..5161
                     /locus_tag="PLT86_12070"
                     /pseudo
     CDS             3870..5161
                     /locus_tag="PLT86_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466123.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
     gene            5379..>5526
                     /locus_tag="PLT86_12075"
     CDS             5379..>5526
                     /locus_tag="PLT86_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOT37390.1"
                     /translation="MTTGSKEAYLALEDGTILEGLSFAAPGLRVGEVVFNTSMAGYQE
                     VLTDP"
BASE COUNT         1151 a         1662 c         1662 g         1051 t
ORIGIN      
        1 cgccgtgcgg ggtctcgcgc tcggcggcga caccggcggc gcgaaggcca tcgttccggg
       61 tgtcgcttcc atcgaaaccg cgcggtacgc gcacgaggag ctcaacagca tcgagagaac
      121 cgcggttgcg tacgtgaaca accctgttcg tcttggcatg gaagaagcgg cgaggctcgc
      181 gaatctcgac atcagcgtga acatcatcat gaacggagac cggcaggttg ccgggctttt
      241 tgcgggagat gtggtgggag cacatcgcgc tgcggtccgg gtcgcccatg aacgttacaa
      301 cacaacactc gcccagaacg ccgatgtcgt ggtctcgaat gcctacccgc agagcgtcga
      361 ggccagcaaa gagctcaaat actgccgcat gagccttcgc gacggcggta ccgcggtcct
      421 gattcaggat acccccgccg gccagcgcaa aattcattat ctgggatgga gcggcgatct
      481 cgaacgccgc gccttgcggc ggcgttcacg cgggctgccg gttccccagg ccgcgcaggt
      541 gatcgtcttc aaccggtggc cgtgcgcact ggatgagatg gattactccc ccgaggtcta
      601 ccacgccaaa acgtgggatg aagttgtcgc ccggctaacg cggattcacg gcgaaggaac
      661 gaaggtcgcc gtgtaccccg ccgccgcact cgcgcacgaa ccgatgccct tgcggatgta
      721 gacgcaactt ttccgggcaa ttgcttccgg caaaaaaaca ggggcatgac gtgaaaactc
      781 atgcccctca cttttttgtg cgtttgggcg agcccgttac tgaggtatca ggctcgtttc
      841 aggcgtcagg tcagcacaga acgtcgccag catctgtgct gattgctcca tatcgctgag
      901 cgcgatggtt tccaccgtcg tgtgcatgta ccgcaaagga aggctcacaa gccccgtcgc
      961 cacgccgctc cgtgtcaact gaattacgtt ggcgtcggtt cccgtgcccc tgggcgcacc
     1021 ttccacctgg tacgcgattt cgttcttttt cgcggcgtca acgagccggt caaacacgat
     1081 cgggcttatg tttgccccgc gggaaatcgc tggcccgccg ccgagattca cctcacccgc
     1141 tttccgtttg tccatgtcgg gcgaatcggt tgcgtgggtc acatccaccg caatccctac
     1201 gtcggggtcg attccgaacg cgctcgtctg cgctcccctc aaaccgactt cctcctgaac
     1261 cgtgctgacg gcgtatactg cagccttgag accagcccgg ttttccgcga tcaaacgcag
     1321 cgcctcggcc accacgaacg cgccggcctt atcatcaaaa gcacgggata ctgcaatgcc
     1381 gttccgcaac tcctcgaacg cgacgttgta ggtcaccgga tcacctatcg caacaagctc
     1441 cttcgcttcg tctttgctct tcaccgcgat atcaatccag tacccttcaa tcttcggctt
     1501 tgggtcatct cgctcctgct gcgtcatcag gtgaattgcc cgttttccga tcacgccctt
     1561 caccggcccg ttcgccgtgt ggatcgttac acgtcggccc ggaacgatcg caacatcgtg
     1621 tccgccgata gcaaggaaat tcagaaaccc ctggtcgttg atgtagttga tcatgaaccc
     1681 aagctcgtcg caatgtcccg caagcataac gcgcggcttt ccgccggggt tgatggccgc
     1741 aatcgcattg ccatgccggt cgcttgttac cgtgtcggcg aatgaagaca cataatctcg
     1801 ccagatgcgc tgcgcaggct gctcgtagcc cgatgggctc ggcgcctcga gcaacgcctt
     1861 gaggaacgaa aggctttctt cacgcatgga tagctccttg agtccgcggg attccgcgat
     1921 gggcgggaac gtagtgcaaa agcagataga tgcaaaaaaa gataggcgag caacgtgatt
     1981 ccgcgcaacg cgtccagccg cacccctttc ctgccccgcg ggtgctcaca cctgtgccaa
     2041 cctttcgttg acgggaactg ctcaacaccg tatcttctaa ataacagcgc gcaacagccg
     2101 aaaacgcact gtgttgcatg gcaggcgatg aaagtcagac gattgtgcag acaggcacgt
     2161 ggcgataccc gccgggcagg atgccccgcg ggtttttttt tgcctggtca ttcggacggg
     2221 gtggctgggt cggggctgat cccgtccgcg aaggctgaaa ttcgagacgt ggagtgaaac
     2281 atgccggaaa cacacgaaca gaccaaaagc tcgggttccc ctggtccggt ggtgatgacg
     2341 gcggcccaga tgtccagggc gctcaatcgt atggttcacg agattcttga acgcaacggg
     2401 ggtgcggaga accttgccct ggtgggagtg cgcactcgcg ggtatccact ggcgctgcgt
     2461 ctcgcgtcgc tgatcgagag agtcgaaggc aagaggccgc ctaccggcgc ggtagacgtc
     2521 acgttttttc gcgacgactt ccgattgcgc gcgaaagcac cccaggaatt caccgacatt
     2581 ccgttcgcgg tcgacaacaa aaacatcgtc cttgttgacg atgtgttctt caccggcagg
     2641 acgacacggg cagcaatgga aaccgttatg gaatttggac ggcccagaac catccaactt
     2701 gctgtgttgg tagacaggaa ccaccgcgaa ctgcccatcc atccagatta tgtcgccaaa
     2761 cgggtggaaa ccgctctgga cgaagtcatc cgtgtccgat tgaaggaaat tgacggggaa
     2821 gatcaggtac ttctcgtcag aggcggagtc acaccatgac caaatggacc aggaagcacc
     2881 tgttggggct cgcggatctc acccgcgaag aaatcggcca gattctcgat tcagccgacc
     2941 agttcgtatc cgttctcgac cgccccatgc gctccgttcc cacgctccgc ggatggaccg
     3001 tggtcaacct gtttttcgaa gacagcacgc gaacccggat atcattcgaa ctcgcggaaa
     3061 agcgactcgg tgccgacatc gtcaatttca gcgtgtccac ttcgagtgtg aaaaagggtg
     3121 aaacgctcag ggacacggcc cggaacattg aagccatgag ggtggacgca gtggtgatac
     3181 ggcaccgctc ggtcggagcg ccgcatttcc tctcacggca catccaggga agcgttatca
     3241 acgccggcga cggctcacac gaacacccga ctcagggctt gttggacctg ttgacgatcc
     3301 ggcgccactt cggccacata gacgggcttc gcgtggcaat cgtcggcgat atccggcaca
     3361 gtcgggtggc gcgatctgat gtctggggat tcaccacgat gggcgcggaa gtcgtcctgt
     3421 gcggccctcc tacgctcttg ccaaaacaca cggataactt cccggcaaag gtgactactc
     3481 gactcgaaga ggcgctcgag ggcgccgatg tagtgtatgc actgcggatt cagcgggaac
     3541 ggcaggccgc ggggttgttc ccctccatca gggaatacag cgcgcagtac ggaatctcga
     3601 gcgaacgcat ggaacgggcg aaaccgaccg ccgtactcct ccaccccggg ccgatcaacc
     3661 gggggtggga actcaccgat gaggtgacgg acgggccgcg ctccctgatc ctggagcagg
     3721 tgacgcacgg ggtggccatc cgcatggcgg tgctctatct cactgcgggg cgagatccag
     3781 cgcagctcga ccttgcgccg aaaccctgaa ctcctggttg atgtcttttc ggtggcggaa
     3841 accaacatta ccgcgaatga gcgaggttgc tgatggaatc actgattctg cgtggcgggc
     3901 atgtcctgga tccttcgcag gggatcgacg agcaggtgga cgtggtaatc gagaacggga
     3961 aagtagccga cattataccg ccggggcgta aggcccgtgg gcgggacgtg aacgtcgaag
     4021 gcatgtttgt tgccccgggc ctcgtggata ttcacgtgca cctgcgcgag cccggttatg
     4081 aatacaagga aacgatcgag acgggcgcga atgctgcggt cgccggcgga ttcacctcca
     4141 tcgtggcgat gcccaacacc gacccgccgc ccgataccgc cgctgcggtg ctgggattcg
     4201 ccgcacgggc gcggaatgcc gcctgtcggg tatatacggt cggaacaatc acccaggcag
     4261 gcaggggcag gaactcagcg aaatggcgga tctggttgcc gctggcgtgg tgggtttttc
     4321 cgatgatggg gcgccggtaa gcaattcccg cgtgctgctc aatgcgatgg aatacgcgcg
     4381 gccgctgggg aaagccatcc tctcgcattg tgaagtgccc gaacttgccg aaggcggaca
     4441 gatgcacgag ggcagggtga gttcggagct cgggatcgcc gggatgccac ccatcgcgga
     4501 atcgttgggc atttctcgcg atatcgcgct tgcggggtac agcggcgcac ggcttcacat
     4561 ttgccacgtc tctgcggccg caagtgtcga cgtcattcga agcgcgaaaa tggcgggcag
     4621 cgcacggatc acggcggaaa cagcacctca ttatctggcg cttaccgacg aggcagtcag
     4681 aacattcgac agcaatgcga aaatgaatcc gcctcttcgc actcgccagg accaggaggc
     4741 gctgaaaaag gcactgcagg atggaaccat cgacgtcatc gccacggatc acgcaccaca
     4801 ttctcacgaa gagaagcagg tggaatttga cgccgcgccg ttcggcatca tcggactgga
     4861 aacctccctg ggtgtgtgtt ggacggaact cgtcaaaacg ggtgttctta ccccgatgca
     4921 actcatcgaa aaaatgtcca ccaaccccgc gcaggtatgc gggcttccgg cgggaacact
     4981 gaagcccggc accgctgccg acgtggtcgt gatcgatccc gacatgcgct gggtagttgc
     5041 cgaacccttc cgttccagat ctcagaacag ccccttcatc ggatgcgagt tgcagggaag
     5101 ggcaatactt accatcgtag gtggcacggt tcgatacgac gtgggcatgc tcgcagaata
     5161 acacacaacg cgcacgcagg ggcgccggtc ggtctcgata acgcttccgc cgggctcaca
     5221 ggtgccaccg ggaatgtgct ccgaggcgcg tgccacgcag tatcacacgg cattcctcgt
     5281 tcgtgatcat gtgcgcattc agtcgcgcgc cggttcgtac agcgttccgg gaatcacagc
     5341 gggaccaaat ctgacgggcg aacttcagca gggaaatcat gacaaccgga tcaaaagaag
     5401 cgtacctcgc gctggaagat ggcaccatac tcgaagggct ctcattcgct gcgccggggc
     5461 tgagggtagg cgaagtcgta ttcaacacct ctatggcagg ctaccaggaa gtgctgacag
     5521 acccct
//
LOCUS       DAOSOO010000204         5514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14878_length_5514_cov_5.683459, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000204 DAOSOO010000000
VERSION     DAOSOO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14878_length_5514_cov_5.683459"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLT86_12080"
     CDS             <1..712
                     /locus_tag="PLT86_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT37391.1"
                     /translation="AFDRGPDAWAEWRDVDGDWTEGEIVHGDIADYPTVERAITGMDA
                     VIHTAVFFPKADDPNDPRPFLINLKGMWNVLETARLAGIRRVVHVGSCGTIHPQGLFY
                     DATVRRAEGDLYGVCKRLQEEMCRQYHEAHGMSTVVLRPDYIVDSRLGIGRFREKLGQ
                     TGSPWRLGWVCRYDLAEACLLAAENPTIEHEILHIVGTPGAEKFCNVARAQQVLGLEF
                     KGDLERFRDPAESGRTEL"
     gene            879..1799
                     /locus_tag="PLT86_12085"
     CDS             879..1799
                     /locus_tag="PLT86_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37392.1"
                     /translation="MTTLHNQSFFWVRGFGYQPSYAPTGLEIWLHFDERLIATELARA
                     NEHFPGTNAIRLWLSFDAWLREPARFVRNFGRVLDILGHLGLRAMPCLFNNWRSLPDF
                     GGINAEMVGYWNSPHREVNYFHRYARDLVTPFLNDERILVWDLCNEPFNSGREEVFVP
                     WLADLHGFVKGTGAVAPVTIGVPPDLHQLELVEPMCDVLTPHLYGASAANDAFIEFAN
                     RVGKPMFAGETGWGSLDDDVRLGTLHRELSSLLERKVGFMIHVLHHSLVADCHRAEFG
                     PVSEVGFMGCIEADGTMRAGYESIRKYFAQ"
     gene            1813..2862
                     /locus_tag="PLT86_12090"
     CDS             1813..2862
                     /locus_tag="PLT86_12090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37393.1"
                     /translation="MSELHHTDVFVSGQDGFAFYRIPVIEVAADGSILAFVEARKHNL
                     GDPGQDKNHIALAMKRSTDGGRTWSPMVIIEDPGEGWSAANPATVFDRDTGRVWLHYL
                     RSKPGRGSEHARPGTDDLQNLVRTSDDNGVSWSEPTDITTVCRDMADPKWRCTVVGPG
                     GGIQDSKGRLMVACWMTEPMRVFAVFSDDHGHTWQRGGFVPGGILGNEDQLVELADGR
                     ILLDFRQAEGDHRWFAESTDGGRTWSQPRVGEPVSPVACAIERFTLKSAGDDKNRIIW
                     SGPKGPGRENLVVRISEDEAASFPNELLVATGKAAYVDAARLKDGSIGLLWEREHYQF
                     ITFTTLDLAFLTSTG"
     gene            2885..4336
                     /locus_tag="PLT86_12095"
     CDS             2885..4336
                     /locus_tag="PLT86_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37394.1"
                     /translation="MTETELLGQLRGIENRVPCGGAGTPRDVTGYASLARRRTWTQRW
                     DADARTSREPHEVTGTVWSDAIERCLAETGSVLIPRMEAPVYLDRPIVLESGARLIVH
                     PETEIRLIVGAVGTCLVRNAGIVFSQDQPVRMCENADKDIVIEGGIWSDQNNEGRGRG
                     GTYDEADSMQGAHGAFLLHNVAGLAVRNARFRDCSAFAIQLGNARDFLLDGVTFDETA
                     DGIHVEGPSERGIIRSVSGKTNDDVVALNAWDWDNSSLTFGPITDVLVEDVESVPGYT
                     WSEVRLLPGTKVFPGGQRVDCDIRRCVFRDIRGMHTFKMYDQPNMFKPEQDFADPIGK
                     MVDLFFSDIVVDGISAAEYYDPSSDAVFDICADVDGMTIRDVRFNYTPGQNDMAANLV
                     SVGPKALTWPRNRERDEGWFEVFNPNANPVVKGLTVDGVFVPHPHNPGRYIPCGDVLA
                     LVHERVAKPNPDFPRTMPRGGTGRGSIVSPVFL"
     gene            4355..5404
                     /locus_tag="PLT86_12100"
     CDS             4355..5404
                     /locus_tag="PLT86_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37395.1"
                     /translation="MHTSDWVRVTETDDRVFIETDRLEAAIPKKNPKRWMTGIEKESF
                     RDKATGFHEVGDGLMVIDWLMEAGSDEEYAEKLYAPDGEGVGRYTWYENETDPERREY
                     ALMAHGSSHRKRMIEGPQLCHRMKPVQPEVIQGGDFLAVKTTYRYEYAAPGRNAGSRW
                     TQLIVFPKGERFFVLMDRIDSVNDSPEMFLRNDTPGCVRHVQGDTFGEMYLSYLSGPN
                     GVRIPSSEFFNSFPPDLKFGYRRDLNRVPERFIRAYHLRDPKTGAAGPWLAGLTLEPS
                     VVYEAWCSQRPGDIIVMIEEVHGKPVKAGESFSAAHIVGFFDTIDEMYAVYDQYKGHT
                     ALDVDENGWRLERVM"
BASE COUNT         1111 a         1713 c         1742 g          948 t
ORIGIN      
        1 ggcatttgac cgcggacccg acgcctgggc tgaatggcgt gacgttgatg gcgactggac
       61 ggagggcgag atcgtccacg gcgacatcgc cgactacccg accgtggagc gggccattac
      121 cggcatggac gccgtaatcc acacggcagt gttcttcccg aaggccgacg acccgaacga
      181 tccgcgcccg ttccttatca acctcaaagg gatgtggaac gtgctcgaaa cggcgcggct
      241 ggccggcatc cgccgcgtcg ttcacgtagg ctcctgcggt acgattcatc cgcagggact
      301 gttctacgac gccaccgttc gccgcgccga gggcgacctg tacggcgtct gcaagcgcct
      361 gcaggaggag atgtgccggc aataccacga agcgcacggc atgagcacgg tcgtgctgcg
      421 ccccgactac atcgtcgaca gccgccttgg tatcggcagg ttccgcgaga aactcggaca
      481 aacaggctcg ccatggcggc tggggtgggt ctgccggtac gaccttgccg aggcgtgcct
      541 cctggcggcg gaaaacccga ccattgaaca cgagatcctg cacatcgtcg gcaccccggg
      601 agcagagaag ttctgcaacg tcgcgcgggc acagcaggtt cttggtctcg aattcaaggg
      661 tgatctggag cgcttccgtg acccggctga atctgggaga acggagttgt gaacctgctt
      721 cacgttcgcc gcacagtgcc actcatgctg tgggcatctt cgttgcgcaa cgctgtccca
      781 tcggtcctat atgccgtatc tccgtcttgt gcccgcgggg tcaccgcgac cgcaagacaa
      841 ccccccaatg aacgacggtt ggaaggaaaa ccggtcccat gaccacgctg cataaccaat
      901 ccttcttctg ggtgcgcggg ttcgggtatc aacccagtta cgcgcccacc gggctggaaa
      961 tctggctcca tttcgacgaa cgcctgatcg ccaccgagct ggcccgtgcg aatgagcatt
     1021 tccccggcac gaatgccatc cgactctggc tctcgttcga tgcctggttg cgcgaacccg
     1081 cgcgtttcgt ccggaacttc gggcgggtgc tggacatcct cggccatctc ggtctgcgcg
     1141 cgatgccgtg cctgttcaac aactggcgca gcctgcccga tttcggcggc atcaacgcgg
     1201 aaatggtcgg atactggaac tcgccccacc gggaggtcaa ctacttccac cgttacgcgc
     1261 gcgacctcgt cacaccattc ctcaacgatg agcgaatcct ggtgtgggac ctgtgcaacg
     1321 aaccgttcaa cagcgggagg gaggaggtgt tcgtcccatg gcttgccgac ctgcacgggt
     1381 tcgtgaaagg aacaggcgcg gtggctcccg tcacgatcgg cgtcccacct gacctgcacc
     1441 agcttgaact ggtggagccg atgtgcgacg tactcacgcc ccacctctac ggcgccagcg
     1501 cggcaaacga cgccttcatc gagttcgcga accgcgtcgg gaagccgatg ttcgccgggg
     1561 agacgggttg gggaagcctc gacgacgacg tacgtctagg aacactccac cgcgaattgt
     1621 cgagcctgct cgaacgcaaa gtgggcttca tgattcacgt tctccaccac agcctcgttg
     1681 ccgattgcca ccgcgccgag ttcggcccgg tgagcgaggt agggttcatg ggctgcatcg
     1741 aagccgacgg caccatgcgc gccggctatg aatccatacg gaagtatttt gcgcaatgaa
     1801 ggagctgcac ccatgtcaga acttcatcac acggacgtgt ttgtctccgg gcaggacgga
     1861 tttgcgttct accgcatccc tgtcatcgaa gtggcggccg atggttccat cctcgcgttc
     1921 gtcgaggcgc ggaagcataa cctcggcgat ccggggcagg acaagaacca cattgcgctt
     1981 gccatgaaac gcagcactga cggcggccgc acgtggtcgc ccatggtgat cattgaagac
     2041 cccggtgagg ggtggtccgc ggccaacccg gcgacggttt tcgaccgcga caccggacgc
     2101 gtgtggctgc attatctccg aagcaaaccg gggcgaggct ccgagcatgc gcgcccgggc
     2161 accgacgatc tccagaacct cgtccggacg agcgacgaca acggggtgag ctggtcggaa
     2221 ccgaccgaca tcaccaccgt ctgccgcgac atggccgatc cgaagtggcg ctgcacggtg
     2281 gtggggccag gcggtggcat tcaggacagc aagggccggc tcatggtggc ctgctggatg
     2341 accgaaccga tgcgcgtgtt tgccgtgttc agcgatgatc acggccacac gtggcagcga
     2401 ggcggcttcg ttcccggggg aattctgggg aacgaagacc agttggtcga gctcgccgac
     2461 ggccgcatcc tcctcgattt ccggcaggcg gagggcgatc atcgctggtt cgccgaaagc
     2521 accgacggcg ggcggacatg gtcgcaaccg cgcgtgggag aaccggtctc tcccgtcgcc
     2581 tgcgcaatcg aacgcttcac tctgaaatcc gccggcgacg acaaaaaccg catcatctgg
     2641 tcagggccca aaggcccggg ccgcgagaat ctcgttgtcc gcatcagcga agacgaagcg
     2701 gcgagtttcc cgaacgaact cctcgtggca acgggcaaag cagcatatgt ggacgcggcg
     2761 cggctgaaag atggcagtat cgggctcctc tgggaacgcg agcattacca gttcatcacg
     2821 ttcacaacgc tcgatctggc atttctgaca tcgaccggat aacgcgcgac gggaggcaac
     2881 actggtgacg gaaacggaac ttctgggcca acttcgcggc atcgagaacc gcgtcccttg
     2941 tggtggcgca ggcacgccgc gggacgtgac cgggtacgca tcactcgcac gcagaaggac
     3001 gtggacgcag agatgggatg ccgacgcgag aacgagccgg gagcctcatg aagtcaccgg
     3061 aaccgtctgg tcggatgcta tcgagcgttg tcttgcggaa acaggctcgg tgctgatccc
     3121 ccgcatggaa gctccggtct accttgaccg tcccatcgtc ctcgagagcg gtgcccggct
     3181 gatagtccac ccggagaccg aaatccgcct catcgtggga gccgtcggaa cgtgccttgt
     3241 gcgcaacgcg ggaattgtct tctcgcagga tcagccggtc cggatgtgcg agaacgcgga
     3301 caaagacatt gtgatcgagg gcggcatctg gagcgaccag aacaacgaag ggcgcggccg
     3361 cggcggcacg tatgacgaag cggattccat gcagggcgcc cacggagcat tcctgttgca
     3421 caacgtcgcc ggattggccg tacgcaacgc gcggttccgc gattgctcag ctttcgccat
     3481 ccagcttgga aacgcgcggg atttcctgct ggacggcgtc accttcgacg aaacggccga
     3541 tggcatccac gtcgaggggc cgtcggaacg cgggatcatc cggagtgtct ccggaaaaac
     3601 caacgacgac gtggtcgcgc tcaacgcgtg ggactgggac aacagcagtc tcacctttgg
     3661 cccgattacc gatgtgctgg tggaggatgt cgaaagcgtg cccggataca cgtggtcgga
     3721 ggtgcggttg ctccccggca cgaaggtctt cccgggcggc cagcgtgttg attgcgacat
     3781 ccgcaggtgc gtgttccgtg atatccgcgg catgcacacg ttcaagatgt acgaccagcc
     3841 caacatgttc aagcccgagc aggatttcgc cgatcccatc ggcaagatgg tggatctgtt
     3901 cttctccgac attgtcgtgg acggcatcag cgcggccgag tattacgacc cctcatccga
     3961 cgccgtgttc gacatctgcg ccgacgtgga cggcatgacc atccgcgacg tccgcttcaa
     4021 ctacaccccg ggccagaacg acatggctgc caacctcgta tcggtcgggc ccaaagccct
     4081 tacctggccg cgaaacaggg aacgcgacga gggctggttc gaggtgttca acccgaacgc
     4141 aaaccccgtg gtgaagggac tgaccgtcga cggcgtgttt gtgccccacc cgcataaccc
     4201 cgggcggtac attccgtgcg gggacgtatt ggctcttgtc catgaacgcg ttgccaaacc
     4261 caaccccgat ttcccgcgca ccatgccgcg agggggaacg ggcaggggga gcatcgtgtc
     4321 accggtattt ctttaattcg aggggactga acccatgcac acgagcgact gggtgcgcgt
     4381 aacagagacc gacgaccggg tattcatcga aaccgaccgg ctcgaagccg ccatcccgaa
     4441 gaaaaacccg aagcgctgga tgaccggcat cgagaaggaa tccttccgcg acaaagcaac
     4501 cggtttccac gaggtcggcg acgggctcat ggtgatcgac tggctcatgg aggccggcag
     4561 cgacgaggaa tacgccgaaa aactctacgc ccccgacggc gagggcgtcg gccgctacac
     4621 gtggtacgaa aacgagaccg atccggaacg cagggaatac gcccttatgg cgcacggcag
     4681 cagccaccgc aagcgcatga tcgaaggccc gcagctctgc caccgcatga aaccggtgca
     4741 gccagaagtg atacagggcg gcgatttcct cgccgtgaag acgacatatc ggtatgagta
     4801 tgccgcgccg gggcgaaacg ccggttcgcg gtggacgcag cttatcgtgt tcccgaaggg
     4861 cgagcgcttc ttcgtgctca tggacaggat cgacagcgtc aacgactcgc ccgaaatgtt
     4921 cctgcgcaac gacacgccgg gctgcgtgcg ccacgttcag ggcgacactt ttggcgagat
     4981 gtatctgagc tacctcagcg gcccgaacgg tgtccgcatc ccgtcaagcg agttcttcaa
     5041 ttccttccct cctgacctca aattcggcta ccggcgcgac ctcaaccgcg ttccggagcg
     5101 gttcatccgg gcctaccacc tgcgcgaccc gaaaaccggt gccgcgggcc cgtggctggc
     5161 cgggctgacg ctggaaccat ccgtagtgta cgaggcgtgg tgcagccagc gccccggcga
     5221 catcatcgtc atgatcgagg aagtccacgg caagccagta aaggcgggcg aatccttcag
     5281 cgccgcgcat atcgtcggct tcttcgacac catcgacgag atgtacgccg tgtatgacca
     5341 gtacaaaggg catacggcgc ttgatgtgga cgagaacggg tggaggctgg agagggtgat
     5401 gtagggggtt ggcgcatgcc agttgatgca tggacagcga agcgcgtaca ttgctgcaaa
     5461 agggtctatg cgggcaacag cgagcaacaa caaagtggaa ggcacgacgc cacc
//
LOCUS       DAOSOO010000205         5513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14882_length_5513_cov_6.575119, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000205 DAOSOO010000000
VERSION     DAOSOO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14882_length_5513_cov_6.575119"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLT86_12105"
     CDS             <1..227
                     /locus_tag="PLT86_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37396.1"
                     /translation="SPARPCEPGSAIARRWRFTGPYRTLKPEKPAGAVLVVPFLDSRL
                     RGNDVIGVGNSKRPGTHGRIRGPGGQAIGV"
     gene            complement(289..861)
                     /locus_tag="PLT86_12110"
     CDS             complement(289..861)
                     /locus_tag="PLT86_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOT37397.1"
                     /translation="MKRSQMNAIIARSREIFQRHGWILPPNPKWDIVDFGYGNFEKFG
                     LVLVNLAEEKEYCEKIMLVLQNQITCAHTHKQKKEDIIARFGTLAIRLWDNAELKCGK
                     PLTVRVNGVPREVISGDIVYITSGERITLTQGLYHEFWAVGEYCVVGEVSTANDDKTD
                     NFFAADDVSRFPPIEEDVPPLARLVWETEG"
     gene            complement(925..1998)
                     /locus_tag="PLT86_12115"
     CDS             complement(925..1998)
                     /locus_tag="PLT86_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37398.1"
                     /translation="MAGTSVALITMADGPHAGAYLGTFSVMKRVDSVHVCDSSGRIFD
                     QAAQLLGKKLVGTSTDVEACLNQGPYAFVLVALDNFSAPAVISQVLDAGFHVFSEKTG
                     ARTAAEWRPLKEKAAKKHLLLGMSYPNRYRPSVQEARRILQAGYLGRLYGFYVQSVAT
                     QAVLRDPKGSWMFRFDRAGGGYVIWLGCHYLDLLRYVTNEEVASVAAQYGRVSPVEFD
                     VEDAVGLAMKLDSGAVGAANFGYYLGPGKIAAGKQSQLTFWGELGWMKLQPGDDETAP
                     LEVYSDHPEFLGTPYRKTFFTHSDVPKAYGSGWGLAFLNDFLAACLGEKEAPVNARDA
                     EAVLEIAEAAYHSATEGKTVAIP"
     gene            2184..3098
                     /locus_tag="PLT86_12120"
     CDS             2184..3098
                     /locus_tag="PLT86_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT37399.1"
                     /translation="MVDLHLHTACSDGREAPEIVVYRASQAGLSAIAVTDHDTVDGSI
                     RAVAAAPDHMVVLSGIELSAAEGESAIHVLGYGIDPFDNALRVLLESMKRDRYVRAGE
                     IVNRLHAMDVPLTLESVEHVAAGSPITRAHVAEALRREQMVATYTEAFERYLSDAQPA
                     FVLQESLSASEAIAAIHRAGGVAFMAHPGVTRRDEIIPSLVKSGLDGIEVYHPNHESA
                     AERFYLNLAEKHGILVSGGSDYHASFRGTDRMGTPYVPREVFDRIVEAIDLRAGTRGS
                     GDKEDSGDGSLVGPPPGARAEAPGPHSG"
     gene            3119..5197
                     /locus_tag="PLT86_12125"
     CDS             3119..5197
                     /locus_tag="PLT86_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT37400.1"
                     /translation="MATIAYSRTVLVPALLSLLCARAGASSPADSVSRALRAGRWQVA
                     ERLLNASETSPDTNLSRMRRGLIYLATNRLREADSVLAAAELPASLAPYREYWRAQAW
                     QRTGNLDQAWAAFDRLAGAGIPAISDSAQFAALQVATAAGNRQRILRAATRLTERSDN
                     LAAAGWLSLMETDSTRWRGAWDALRTRYPRTVEAYRAAEQAQQRGWQPSGADKLALAD
                     IYSRRGQVATAMRWWNDALQDPALASRRNEIRFTAAEYLVNRRQLDDAETYLTALIAD
                     SSGNSFLPRALRLYASLHLKANSEAQMRVWERTFVERFPRHSQVPTALLAIGMSWERE
                     GDFSSAIATYEEIINRFPDNAQTEEARWRLGFCQFRLGRYDAARHAFSPLARTARDFV
                     IKDQAGYWLALALKAEGQEDSARTIWRRTAEFSPRSFYAVASAVACGSPVVKDAREPL
                     QASPPNRARISAWNGFPEAEWLALLGQWRFARAALEQRIVGDANTIAEMESAADAFEW
                     LADYGTSLRWRWKAMWKRTTDDRHHELAPELLARVYPDFFHADVVAAAQEHAIEPALV
                     WAIIRQESVFDPEARSGADARGLMQIIPPTGAKLAREEGLSGYTAQDLYDPGLNIRLG
                     TRYAAQQLRQFGSIDLMAAAYNAGPHNAVRWKLDDPDVQREAISFGETRKYVKLVLRN
                     YYVYKALYGD"
BASE COUNT          955 a         1846 c         1686 g         1026 t
ORIGIN      
        1 gatcccccgc ccggccctgc gaaccgggct ccgctattgc gcgccgttgg cgcttcacgg
       61 gtccataccg caccttgaag cctgaaaagc cggcaggcgc ggtgctcgtt gtgccgtttc
      121 tggattcccg ccttcgcggg aatgacgtta tcggtgtggg gaattcgaaa aggccgggaa
      181 cgcatggccg aatacgcggg ccgggcggac aggccattgg agtgtagaag cttcggacag
      241 gcaggtgagt tgtcccgttg ccgccgcagt ccaagggtgt caatctccct acccctccgt
      301 ttcccacacc agccgggcga gcggcggcac atcctcctcg atgggcggga agcgcgatac
      361 gtcgtctgct gcgaagaaat tgtccgtctt gtcgtcgttg gccgtggaga cctccccgac
      421 cacgcagtac tcccccactg cccagaattc gtgatacagc ccctgcgtca gggtgatccg
      481 ctcgccagag gtgatgtaga cgatatcccc ggagatcacc tcccgcggca caccgttcac
      541 ccgtaccgtc aacggtttgc cgcatttgag ctcggcgtta tcccacaggc ggattgccag
      601 tgtgccgaac cgggcgatga tgtcctcttt cttctgtttg tgggtatggg cgcaggtgat
      661 ctggttctgc agcaccagca tgattttctc gcagtattcc ttctcctccg cgagattgac
      721 gagcacaagg ccgaattttt cgaagttgcc gtacccgaaa tccacgatat cccacttcgg
      781 gttgggtggc agaatccagc cgtggcgctg gaaaatctct cttgaacgcg cgatgatcgc
      841 gttcatttgc gagcgtttca tgatgtctcc tttccccgtt ccgtgcgatg gcgcgttgcg
      901 gtgttctggt ttcttccgcc tcggttacgg aatcgccacc gttttgcctt ctgttgccga
      961 atgatatgca gcttccgcga tttccagcac cgcctcggca tccctggcgt tcacgggcgc
     1021 ttccttttcg ccgaggcacg ccgcgaggaa atcgttgagg aatgcaagtc cccagccgct
     1081 gccgtacgct ttcggcacgt cactgtgcgt gaaaaatgtc ttccggtacg gcgttccgag
     1141 gaactccgga tgatcggaat agacctccag cggcgcggtt tcgtcgtcgc cgggctggag
     1201 cttcatccac cccagctcgc cccagaatgt gagctgcgat tgcttgcccg ccgcgatttt
     1261 ccccgggcca aggtagtacc cgaagtttgc cgcacccacg gcgccgctgt caagtttcat
     1321 ggcaaggccc accgcgtcct ccacatcgaa ctccaccggg cttacccggc catactgggc
     1381 ggccacgctg gctacctcct cgttcgtcac ataccgcagc aggtcgaggt aatggcaacc
     1441 cagccagatc acgtaccctc cgcccgcccg gtcgaaccgg aacatccagc ttcccttcgg
     1501 gtcgcgcagg actgcctgcg tggccactga ctgcacgtag aacccgtaca atcgcccgag
     1561 ataccccgcc tgaaggatgc gtcgcgcctc ctgcacgctc ggtcggtacc ggttcggata
     1621 cgacatcccc agcaggaggt gtttcttcgc tgctttttct ttgagcggcc tccactccgc
     1681 cgcggtccgc gcgccggttt tttcgctgaa cacgtggaag cccgcatcga gcacctgcga
     1741 aatgaccgcg ggagcggaga agttatccag cgccaccagc acaaaggcgt acggcccctg
     1801 attcaggcac gcctccacat ccgtggaagt gccgaccagc tttttcccga gaagctgggc
     1861 ggcctggtcg aaaattctcc cgctggaatc gcacacgtgc acggaatcca cccgtttcat
     1921 cactgaaaac gtgccgagat aggcgcccgc atgcgggccg tcggccatcg tgataagggc
     1981 gactgaagtg ccggccatgg aatcctccct gggaaaaacg tggtgtgcat gtaagaaaac
     2041 gctctcccgc gctctgacac aaggacacgc cccgctccgc gcctggggca gacctgcgcc
     2101 cgcgttgacg gagtgcgcgc gcgcacgtac tttcgttaac ctctgaaatc ggcccttccc
     2161 cgcgccgacg aaaggcgtac gtcgtggttg atctgcacct ccatacggcc tgctccgatg
     2221 gccgggaagc accggaaatc gtcgtgtacc gcgccagcca ggccggtcta tcggccatcg
     2281 cggttaccga ccacgatacg gtggacggtt ccatccgggc agtcgccgcc gctccggacc
     2341 acatggtggt cctttcgggg atcgagctga gcgcggccga aggggaatcg gccatccatg
     2401 tcctcgggta cggcatcgac cctttcgaca atgccctccg tgtgttgctg gagagcatga
     2461 aacgcgaccg ttatgtccgc gccggggaga tcgtcaaccg gctccacgcc atggatgtgc
     2521 ctctcacgct ggaatccgtg gagcacgtgg cggcgggctc gccgatcacc cgggcgcacg
     2581 ttgcggaggc gttgagaaga gagcagatgg tggccacgta caccgaggcg ttcgagcgct
     2641 acctttccga cgcacaaccg gcgttcgtgc tgcaggaaag cctctccgct tccgaagcga
     2701 ttgccgccat ccaccgcgcc ggcggcgtgg cattcatggc ccatcccggg gttacccgcc
     2761 gcgacgaaat catcccttcg ctcgtcaagt ccgggctgga cggtatcgaa gtctatcacc
     2821 cgaatcatga atcggcggcg gaacggtttt acctcaatct tgccgagaaa cacggcatcc
     2881 tcgtttccgg cgggtccgac taccacgcct ctttccgggg aaccgatcga atgggaacgc
     2941 cgtatgtacc gcgggaagtg ttcgatcgta tcgtggaagc cattgacctc cgcgcgggca
     3001 cccggggttc cggagacaaa gaggattccg gcgacggatc gctcgtcgga cccccacccg
     3061 gcgcacgtgc cgaggcaccc ggcccgcatt ccgggtgacc ggcaggagct ctgcgttcgt
     3121 ggcaacaatc gcgtacagcc gtaccgttct tgttcccgcc ctgctctctc tgctctgcgc
     3181 gcgggccggc gcatcttccc ccgcggactc ggtttcgcgc gctctgcgtg caggccggtg
     3241 gcaggtggcg gagcgccttc tgaacgcctc cgagacctct cccgacacga atctctcgcg
     3301 gatgcgccgt ggactcatct acctcgcgac gaaccgcctg cgcgaagcgg actctgttct
     3361 tgccgcagcg gagcttcccg cctcactggc gccgtaccgg gagtactggc gcgcccaggc
     3421 atggcagcgc acgggcaacc tcgatcaggc gtgggcggcc ttcgaccgcc tcgcgggagc
     3481 cggtatacct gccatcagtg attccgccca gttcgccgcg ttgcaggtgg ccaccgcggc
     3541 gggcaacagg caacggatcc tgcgtgcggc cacccgtctg acggaacgga gcgacaatct
     3601 tgcggccgcg gggtggcttt cccttatgga gacggactcc acgcggtggc gcggggcatg
     3661 ggatgcgctt agaacccggt acccgcgcac cgttgaggcg taccgcgcag cggaacaggc
     3721 gcaacaacgg ggatggcagc cctccggcgc cgataagctg gcgctggccg acatttactc
     3781 ccggcgcggg caggttgcca cggccatgcg ctggtggaat gatgcgcttc aggaccccgc
     3841 ccttgcgagc cggcgcaacg agatacggtt caccgccgcg gagtaccttg tcaaccggcg
     3901 ccagctcgac gacgctgaga cgtatctgac ggcgctcatt gccgattcga gcgggaattc
     3961 atttctgccg cgcgcgttgc gcctgtacgc atcgctgcac ctgaaggcga acagcgaggc
     4021 gcagatgcgc gtgtgggagc gcacgttcgt ggaacgtttc ccgcggcaca gccaggttcc
     4081 caccgccctt ctcgccatcg gcatgagctg ggagcgggag ggcgatttct cctccgccat
     4141 cgcgacgtac gaagagatca tcaaccggtt ccccgacaac gcgcagaccg aggaagcgcg
     4201 atggcggctt ggcttctgtc agttccgcct tgggcgctac gatgccgccc ggcacgcctt
     4261 ttcccctctc gctcgcaccg cacgggattt tgtcatcaag gatcaggcgg gttactggct
     4321 tgcgctggcc ctgaaggcgg aagggcagga agactccgct cgtaccatct ggcggcgcac
     4381 ggcggagttc tccccccgga gcttttacgc cgtcgcatcc gcggttgcgt gcggctcgcc
     4441 agtggtgaag gatgcacggg agcctctgca ggcatccccg ccgaatcgcg cgcgcatcag
     4501 tgcctggaac ggatttccag aagcggaatg gctggccctc ctcggccagt ggcgctttgc
     4561 acgggcagcg ctggagcagc gcattgtggg cgacgcgaac accatcgctg aaatggaatc
     4621 ggcagccgat gcattcgaat ggcttgccga ttacggaacc tccctcagat ggcgctggaa
     4681 agccatgtgg aaacgtacca ccgacgaccg acaccacgag cttgcgcccg agcttctcgc
     4741 acgggtctac ccggacttct ttcacgcgga cgtcgtcgcg gcggcgcagg aacacgcaat
     4801 agagcccgcg ctcgtctggg cgatcatccg gcaggaaagc gtgtttgacc ctgaggcgcg
     4861 gtcaggggcg gatgcccgcg gcctgatgca gatcatcccg ccgacgggcg caaagctggc
     4921 gcgggaagaa ggcctctcgg ggtacaccgc gcaggacctc tacgaccccg gcctcaacat
     4981 ccgcctcggc acgcggtatg ccgcgcaaca gctccgccag ttcggcagca tcgacctcat
     5041 ggccgccgcc tacaacgcgg gcccgcacaa cgccgtgcgg tggaaactgg acgaccccga
     5101 cgttcagcgc gaggccatct ccttcgggga aacgcggaaa tacgtgaagc tggtgctcag
     5161 gaactactac gtctacaagg ccctctacgg cgattgaccc acgccccttc gcgttcttcg
     5221 tgccttcgcc tgcccctccc gacctcgact attgaccccg cggtttgttt ccggtatcaa
     5281 ttggcgcata cacgagagga cggtgttccc ctccgcgttc gacgccttcc cgcgagatcc
     5341 caaaaggaga catcgcgatg aacgcacgca aagagaacgt ctgcacgttg gtggccatcc
     5401 tgctcaccgg cgcagcgcac gccggggccg gcacgtggac ttcgggcgcg cctctgccgt
     5461 cggcgcgaca gggcgctgtg gccgccatca ttggaggcaa aatctacgta gta
//
LOCUS       DAOSOO010000206         5498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_14939_length_5498_cov_4.797354, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000206 DAOSOO010000000
VERSION     DAOSOO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14939_length_5498_cov_4.797354"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLT86_12130"
     CDS             <1..862
                     /locus_tag="PLT86_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOT37401.1"
                     /translation="DWILDRLRPNDMMLISPGGLHPAPDAPGSHTPEWERAFSQYLPL
                     WVNHLAEKGFGYERWAFYPVDEPGLLGGKLIDELEYQARLFKRIDPRVQVYTDPFKGM
                     TVADLRRVLDVVDIYQLSFGSVLTEPSRERYNYLKTTGRTLWTYEAYGGVKDMVGVKY
                     YWEMIWTAWELGLTGVGYWSYCTRGVDLWQGPNPNNNDWEMVYGGARTPVPSVRVQAI
                     RIGIEDYARMWAVQNAAETARNAGDMNRSKMLAEALEEIVRAARDSEWNPVHVASLRR
                     KLSALAADDT"
     gene            878..1531
                     /locus_tag="PLT86_12135"
     CDS             878..1531
                     /locus_tag="PLT86_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37402.1"
                     /translation="MSESMYAEFLASLNDSNQGTRAVLTLACVWLLVDMIRGAPWAST
                     ALKAVFASSALTTGLFFYPTLAKHTGEHRETLLFWGAMFLLSSALGFADTILGRTQIR
                     LPRSGWPLAVVLVFCTAGIGFPAVEWLGGRTYRAFQAFALAPAPYALVVLPLLAASAH
                     SSLLGVAWLISVFVASADVALVSRLFGLPYWHPIAMFAVIVSAYFAFRRPISAEHKP"
     gene            1618..2178
                     /locus_tag="PLT86_12140"
     CDS             1618..2178
                     /locus_tag="PLT86_12140"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT37403.1"
                     /translation="MSDYRGHVVAGVVWFLTLAAVAFLLLPELAGSQGRWFVLSGWGF
                     VAQLAVAVLAALWPDVDTASRGRKVFYRVLLVFSLYSMARSQWKMAAVIGLAGILPAL
                     GNHRGWTHTVWAAVLVPLPIVLLPLYVSDDGQWLRTPDFQRISVGLPFYVAAMVGLFS
                     HLAADGFFGLLLARISGGSGKGTRRN"
     gene            2265..3125
                     /locus_tag="PLT86_12145"
     CDS             2265..3125
                     /locus_tag="PLT86_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37404.1"
                     /translation="MTRFEKRCSNAVCGGTRIPSIACAFMATLVGILVLSSCGTRRAR
                     VHGTMYVPPGLQGNAQWGEVWLVHDSPELEYDLKERERLVTLALLGKDVEFLRRQLPA
                     LIQLGVLREKINILNRGQIPTAITLPPRREITALTPFDSIFQGIVVESRPATAPELAA
                     GLDKLKDELRDSVEAIHRRLQTSMDSLRAARAEQKRIMFAGANGSATPKRVMAAPVGL
                     DGRFSFSDVPPGKYRLHAQYRLQQWFVSVPLEVGRSDVQKDIPRLPGVLLDSRAIINT
                     EALCAKILAL"
     gene            3149..3568
                     /locus_tag="PLT86_12150"
     CDS             3149..3568
                     /locus_tag="PLT86_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37405.1"
                     /translation="MAPLRWVIGWFALTPVEILVLAAVSATVFWQALPRYKEWTFRQL
                     VAEETGDVTQLNYALSGDTGLFVRCPARLDDCPDGSTTETCSLFSALSPEFRVASAWR
                     KENGDYLGPAGGRYRYLPGECRLAPVGKESQGGKRRH"
     gene            complement(3584..4069)
                     /gene="greA"
                     /locus_tag="PLT86_12155"
     CDS             complement(3584..4069)
                     /gene="greA"
                     /locus_tag="PLT86_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT37406.1"
                     /translation="MNSIYMTREGRAKLEEELRFLKVEERPRIKRQIVEAREKGDLTE
                     NAEYDAAKEEQGRLEAKIAKLQDQLSRAIVLDDQSLPEGKAHIGRRVDLEDLNTGKRF
                     SYTLVSEAESDFASGKISTGSPVGRGLVGHGAGEEVTVQVPAGTKRFRILRVVSSADT
                     Q"
     gene            complement(4273..5457)
                     /locus_tag="PLT86_12160"
     CDS             complement(4273..5457)
                     /locus_tag="PLT86_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOT37407.1"
                     /translation="METAKVTISAGPHDRHFVPIRTRVPVPQDARSAVVEPYVASPPK
                     KGYIPAPAVVAQLGEREADGTADVTFILPYLARNAAATFQITFLPSPAEEKAILAPQP
                     NGDIEVTISGTRLGRYHADDKWARPFLHPVMGPFGHSVTRGWPVEPRENESQDHHHHK
                     SLWVAHGSVNGVDNWSEETGHGRQIQKQVLNCQSGPVFGVLETVNDWVDNSGKKVCED
                     RRKWCFWAMPQGLRMFDLEVEFRATEGPVVFGDTKEGGIIAVRVATCMDVPRTGRITN
                     ADGGTNEPETWGKRSPWCDYSGLDEGGNEVGIALFNHPKSFRYPTYWHVRNYGLMTAN
                     PFGLSEFYGDKSRNGSHTLGAGETLAFAYRVYVHAGDVVGGQVADAFHGYINPPSVSV
                     VA"
BASE COUNT         1037 a         1642 c         1610 g         1209 t
ORIGIN      
        1 cgactggatt ctggatcggc tgcgccccaa cgatatgatg ctcatcagtc cgggtggcct
       61 tcacccggcg cccgacgcac ctggttccca cactccggag tgggagcgcg cgttttcgca
      121 gtacctcccg ctctgggtga atcaccttgc agagaaaggt ttcgggtacg aacgctgggc
      181 tttctacccg gtagatgagc ctggactgct cggcggcaaa ctcattgacg agctggaata
      241 ccaggccagg ctgttcaaaa gaatagatcc gcgagtccag gtgtacaccg atcctttcaa
      301 gggtatgacg gttgccgacc tgcgaagggt gcttgacgtc gttgacattt accagttgag
      361 ttttggatca gtccttacgg agccatctcg ggaacgttac aactacctca aaaccaccgg
      421 aagaacactg tggacctacg aagcatatgg cggcgtcaag gatatggtgg gagtgaagta
      481 ttactgggag atgatctgga cggcgtggga actcggcctt accggtgtcg gctactggtc
      541 gtactgcacc cggggggtgg acctgtggca ggggccgaat ccgaacaaca atgactggga
      601 gatggtgtac ggcggagcac gcacgccggt tccaagcgtg cgcgtccagg cgatacgcat
      661 cggtatcgag gactacgctc gtatgtgggc ggtccaaaat gcggcggaaa cagcgcgaaa
      721 tgccggcgac atgaatcgtt caaaaatgct tgccgaggcg ttggaggaga tcgtccgtgc
      781 ggcacgggac tccgagtgga acccggttca cgtagcgtct ctgcgtcgca aactgagtgc
      841 gctcgcggcg gacgacacct gattcgggcg cacgcacatg tccgaaagca tgtacgcgga
      901 gttcctcgcg agtctcaatg attcaaacca gggtacgcgc gctgttctca cacttgcctg
      961 tgtgtggctc cttgtggata tgattcgggg tgcaccgtgg gccagcacgg cactgaaagc
     1021 agtgttcgcc agttctgccc tcacaacagg attgtttttc tacccgacgc tcgcaaaaca
     1081 cactggcgaa caccgtgaaa ccctcctctt ctggggcgcg atgttcttgc tttcgagcgc
     1141 gctcggattt gcagacacga tcctcggcag aacacaaatt cgcctccccc gttctggctg
     1201 gcctctcgcg gtagtgctgg tcttttgcac agcgggaatc ggattccccg cggtcgagtg
     1261 gttgggcggg cgtacctacc gtgcgtttca ggcttttgcg ctggctcctg ctccgtacgc
     1321 gcttgttgtc ctgcctcttc ttgctgcatc ggcccacagt tcgctgctcg gagtcgcgtg
     1381 gctcatttcc gtgttcgtcg cgtccgctga cgtggcgctg gtctcccgcc tgttcgggtt
     1441 gccgtattgg caccccatcg ccatgttcgc cgtgattgta tcagcctatt ttgcgtttcg
     1501 tcggccgata agtgcggaac acaagccctg accacatttg cacgaaagca aggaaacgat
     1561 gtacattgct tcgttgaggg tgccgacaga actgccgaga cgcacccggg gcatgtcgtg
     1621 agcgattacc gtgggcacgt cgttgccgga gttgtgtggt tcctgacgct cgcagccgtg
     1681 gcttttctgc ttctgcccga gctggccggc tcccagggtc ggtggttcgt cctgagtggc
     1741 tgggggttcg tcgctcagct tgccgtggca gtgctggcgg cactttggcc tgacgttgac
     1801 actgccagcc gcgggaggaa agtgttctac cgcgtgctgc tcgttttttc tttgtacagc
     1861 atggcgcgca gccagtggaa aatggccgcg gtgattgggc tcgcggggat tctgcccgcg
     1921 cttggaaacc acagggggtg gacgcacacg gtgtgggccg ccgtgctcgt tccccttccg
     1981 atcgttctgt tacctctgta tgtttcggac gacgggcaat ggttgcggac gccagacttc
     2041 caaaggattt cggtggggtt gcctttttac gtggcggcca tggtggggct tttcagtcat
     2101 ctggccgctg acggcttttt cggcctgttg ctggcccgta tcagcggcgg atctgggaaa
     2161 ggtacgcgca ggaattgaga gcacaaaaga accccgactg aaaaaaatgt gcggctcgcg
     2221 ggccgcctca atgtgataga tacgtgctcg tcaggagatt cgcaatgacg cgattcgaaa
     2281 agcgatgctc gaatgcggtg tgcggtggaa cccgcatacc gtccattgcg tgtgccttca
     2341 tggcgacgct tgtcggcata ctggtgttgt cttcgtgcgg cacccggcgc gcccgtgtcc
     2401 atggaacgat gtatgttcct cccggcctgc aggggaacgc gcaatgggga gaggtgtggc
     2461 tcgttcacga ttcgcccgaa ctggaatacg acctgaagga gcgcgaacgc ctggtaaccc
     2521 tcgcgcttct tggcaaagac gtggaattcc tccgcaggca gttgccggcg ctcatccaac
     2581 tgggagtcct tcgcgagaag atcaatatcc tgaaccgggg gcaaatcccg actgcaatca
     2641 ccctgccgcc gcgtcgtgag attactgccc tgacgccgtt tgattccatt ttccagggca
     2701 ttgtcgttga aagcaggccg gcaactgcac cggagttggc cgccggactg gacaagctca
     2761 aggacgaact ccgcgactca gttgaggcaa tccaccgacg actccagaca tcgatggaca
     2821 gcctgcgggc ggcccgggcg gagcaaaagc gaatcatgtt cgccggcgcc aacggttccg
     2881 ccacccccaa acgcgtgatg gccgccccag tcgggctcga tggcaggttc tcgttctccg
     2941 atgtaccccc tggaaaatac cggcttcacg cgcagtacag actccagcaa tggttcgtct
     3001 ccgttcccct tgaggtcggc cggagtgacg tccagaagga catacccagg cttccgggcg
     3061 ttcttcttga ttccagggcc atcatcaata ccgaggctct gtgcgcaaag atcctcgctc
     3121 tttgagggaa atgatcgaca ggaggcgttt ggccccactg cgttgggtta taggatggtt
     3181 cgcgctgacg cccgtcgaga tactggtttt ggcggcagtc tccgccacgg ttttctggca
     3241 ggccttgccg cggtacaagg aatggacgtt ccgtcagctt gtcgccgagg agaccggcga
     3301 cgtcacccag ttgaactacg ctctgagcgg cgacacaggc ctttttgtga ggtgtccggc
     3361 gcgcctcgat gactgcccgg acggatcaac caccgaaacg tgctcgcttt tctctgccct
     3421 gtctcccgag tttcgcgtcg cttctgcatg gcgcaaagag aacggcgatt atttggggcc
     3481 ggccggcgga agataccgat atcttcctgg cgagtgcaga ctcgccccgg ttgggaaaga
     3541 atctcagggg ggcaagcggc gtcattgacc aacgagcgca accttattgc gtatctgctg
     3601 aagaaacaac gcggagtata cggaatcgtt tcgttcctgc gggcacttga accgtaactt
     3661 cctccccggc tccatgtccc acaagccccc tccccactgg tgaaccggtc gagatcttcc
     3721 ccgacgcgaa gtcgctttcc gcctcggata ccagcgtgta gctgaatcgt ttgccggtat
     3781 tcaggtcctc gaggtccacc cggcgcccga tgtgcgcctt cccttcaggc agactctgat
     3841 cgtccagaac aatcgcgcgg gaaagctggt cctgaagttt ggcgatcttc gcttccagac
     3901 gtccctgctc ttcctttgca gcatcgtatt ccgcattttc ggtgaggtca cccttctccc
     3961 gggcttccac aatctggcgc ttgatccggg gacgttcctc gactttgagg aaacgcaact
     4021 cttcttccag cttggctcgt ccttcccggg tcatatagat gctgttcatc aaagggactc
     4081 cctgattatg gccactctgc ccgcctttcc tgcggctgcc cgttccgaaa cgtaaaacaa
     4141 aacctgcacc gacgggcaga gtcggtgcag gaaaatgtgc acttgagtgt aaccgaaaag
     4201 gacgttcagg tcaagccttg cgcgctgaac ccctcgtcac cggcttctct ccattgccgc
     4261 agctcgcctc tctcaggcaa ccacgctgac ggacggggga ttgatgtagc cgtgaaacgc
     4321 gtcggcaacc tgcccgccca ccacgtcgcc agcatgcaca tacacccgat acgcgaatgc
     4381 aagtgtttca cccgcgccga gcgtgtggga accgttgcgg gatttgtcac cgtagaattc
     4441 actcaacccg aacggattgg ctgtcatcag gccatagttg cggacgtgcc agtaggtcgg
     4501 ataccgaaag ctttttggat gattgaaaag tgcgattcca acctcattcc cgccttcgtc
     4561 gagaccggag taatcgcacc agggcgacct cttgccccac gtctccggct cgttcgtccc
     4621 gccatcagcg ttcgtaatac gccccgttcg gggaacatcc atgcacgttg caacccgaac
     4681 cgcgatgata ccgccttcct tcgtatcgcc gaacacgacg gggccttcgg tggcacggaa
     4741 ctcaacttcg agatcaaaca tgcgcaggcc ttgcggcatc gcccagaaac accatttgcg
     4801 tcggtcttcg cacaccttct tcccggagtt gtcaacccag tcgttcaccg tttcgagcac
     4861 gccgaacacc ggtccactct ggcagttcag aacctgtttc tgaatctgcc tcccgtgccc
     4921 cgtctcctcc gaccagttat cgacaccgtt cacactgccg tgcgcgaccc acaaagactt
     4981 gtgatggtgg tgatcctgcg attcgttttc acgaggttcc accggccacc ctcgggtaac
     5041 ggaatgcccg aacggcccca tcaccggatg caggaaaggc ctcgcccatt tgtcgtcagc
     5101 gtgataacgg ccgagacgag taccggaaat ggtaacctca atgtcgccgt tgggttgcgg
     5161 cgccaggatc gctttttcct ccgccggtga gggcagaaaa gtgatctgga atgtcgcggc
     5221 ggcattcctc gccagataag gcagaatgaa cgtcacgtct gctgtcccgt cggcttctcg
     5281 ttcgccaagt tgcgccacca cggcaggcgc agggatgtac ccttttttcg gagggctcgc
     5341 cacatatggt tcgacaaccg ccgaacgagc atcctgcggg accgggacac gagttcggat
     5401 tggaacaaaa tgccggtcat gcggaccggc cgatatcgtc acttttgctg tttccataga
     5461 gcaagctcct gcacgtgagg cggattactg aatcgaac
//
LOCUS       DAOSOO010000207         5474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15018_length_5474_cov_5.058129, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000207 DAOSOO010000000
VERSION     DAOSOO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_5474_cov_5.058129"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLT86_12165"
     CDS             complement(<1..472)
                     /locus_tag="PLT86_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT37408.1"
                     /translation="MSDVWLFFDIGDTLVNEDKLRFQIFKSLQKAITVETGHEFPFQE
                     LLALRERRLLEGELGVHYGIAKELLSPAGYDAWVEGVATLIRNASQSELHPIPGAVNI
                     LGQLSRYSLGIIADQPAHLLSQFANWGFEGVFSVVALDSLTGFHKPDTRLFEWAL"
     gene            complement(469..1464)
                     /locus_tag="PLT86_12170"
     CDS             complement(469..1464)
                     /locus_tag="PLT86_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT37409.1"
                     /translation="MSAASSPRRHELSPIHGGASGRRYFRLSLAAQASDAAATYVVMV
                     VPTVEVGTFDAFCSNGEYLRSQSIPAPAILARWRDQGIALLEDFGTRTLTQVIASEQE
                     NRCALLEGAIGLLVRLHTCRQNDAFPCPAFQLHFDEEKWRFEYRFHVREWLIARHWKI
                     EPTCAESKILDRAFAWLGARLSREPRVFTHRDYQSSNLLVRHDGSYGLIDFQDARQGL
                     RQYDLASFLYDSYVDLNETERSELSNMYASMAGLTIPEDEHRHLVTISAIQRKLHDAG
                     AFAYAAYHREKTEYLCYLAPAVTQSVTLMAQIPHLREAGRVLSDYAARATGGTSA"
     gene            complement(1477..2247)
                     /locus_tag="PLT86_12175"
     CDS             complement(1477..2247)
                     /locus_tag="PLT86_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT37410.1"
                     /translation="MRAMILAAGLGTRLLPYTKSLPKPLLPVNGKPLIAYPLGLVKEA
                     GVKDVVVNVYHLADQIESALKACSTMGLNIRFARERNLLETGGGLSNAQGFLGNETFF
                     LLNSDVVCDIDLKPVLEAHIRSGAIATMVLVEREGQSQYAPVVWDSRSKHVKDLRGEL
                     GVRTKCSRSLTFAGIHVMEPAVFDYLLKRKESIIDAFYLPALREGRHIHGYLHSGYWA
                     DAGTVQDYVRVRRDLAGVRFSHLKPVPLSAPISLDIES"
     gene            complement(2269..2709)
                     /locus_tag="PLT86_12180"
     CDS             complement(2269..2709)
                     /locus_tag="PLT86_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37411.1"
                     /translation="MAGRTVKARAPDAGKGGGTRMHAVAVRTTVAEYDLLDPRAPASA
                     IQACFQKPEVSWDASALEPGEVEAIQRATVRGAATQTIDIIREDRAVWVTYSVTMDFD
                     FNPNALNYFGVSIEEIIVRGCRVSFPIDENIVDLVEEKTTTRVT"
     gene            complement(2691..3746)
                     /locus_tag="PLT86_12185"
     CDS             complement(2691..3746)
                     /locus_tag="PLT86_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37412.1"
                     /translation="MSIGIGIIGTGGISNSHVRGYQANASLGKIVAVADIDGARAEEA
                     ASRWGVDYFTTDYREILARDDVDVVSVCTYNQAHRQPTVDALAAGKHVLCEKPMAATL
                     DDAKAMAKAARDSGLILMIAIHSRYSPQTIAAKKIIDSGALGRVYYGETISTRRRWIG
                     SGSFARQETAGGGAVVDIGVYSLDTALHLLGHPKPKRVSGTTCMAITKDPSAQVYGGH
                     SWDVDRTNVEEFGAAWIRFENGLCITFKTSWAIHGNSMGRSFFLGEKGGVALGPLEYY
                     GDQFGMMVNVIPQDLPPEEDRFVLETRDFLECVRDGRPSPIPPEQVVVTNVIMDALYR
                     SAAADEEVEVTPWQDVL"
     gene            complement(3915..5336)
                     /locus_tag="PLT86_12190"
     CDS             complement(3915..5336)
                     /locus_tag="PLT86_12190"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOT37413.1"
                     /translation="MPFTPLSAIRSSLLNPSDLEQIEEAAYRILEQVGIKVQEDLVPT
                     LRQKGMCFHDGRVVFDRRITRTFLEQERAKGGKVSGMDATPPSTQEITLGVSQYSQHV
                     RDAGTGQIRPYTVSTLIEAAKTVDVLADQGVRAGAPGTPMDVPPDLQPVSIYYLSLTY
                     TRYGFQPPDPRSVAACEYVLDMAETVGHPVTLLPVYLVSPLTLAGDSLAAVLAFEKRI
                     ASTWVVSMPSAGATAPQATGEAFALAAAEVIGSAMIMHELCDLDVGWEIQIFPFDMRA
                     LSIVFGSPENLLMQLASAEVDAYFHGTQWKPSAANIHTMAKTPGPQAAAEKASIMTAG
                     ALLGARHFVCAGTLSLDEIFSAEQLLLDCEIASHVEAVVRGLDTSCDPDACVQDVIEG
                     SSRGFLGLDSTLQRYRESCWYPRFFERGLLAAWQDSGSRELNERLREEARRLPSRHAF
                     ALPEEVQRELDRIYRRAEEHFSI"
BASE COUNT         1123 a         1624 c         1548 g         1179 t
ORIGIN      
        1 tgagtgccca ctcgaacagg cgtgtgtccg gtttgtgaaa accggtcaag gagtccagag
       61 cgacaaccga aaagactccc tcaaatcccc agttggcgaa ctgactgaga agatgtgcgg
      121 gctgatccgc gatgataccg agcgagtacc gggataactg cccgagaatg ttcaccgcgc
      181 cggggatggg gtgaagttct gactgtgacg cgttccggat gagtgttgcc acaccttcga
      241 cccaggcgtc gtaccccgcg ggcgaaagca attccttcgc gatgccgtaa tgaacaccga
      301 gctcgccttc aagaagccgc ctttcccgca aagccagcag ctcctggaag ggaaactcgt
      361 gccccgtctc aacagtgatg gctttttgca gagatttgaa aatctggaac ctgagcttgt
      421 cttcgtttac aagggtgtcc ccgatgtcaa aaaacagcca tacgtcgctc aagccgacgt
      481 tcctccggtg gcgcgcgcgg cgtaatcaga gagtacgcgg cctgcttcgc gaaggtgtgg
      541 aatctgagcc atgagcgtta ctgactgggt taccgcgggt gcgaggtagc acaggtattc
      601 ggtcttttcc ctgtgataag cggcatacgc gaacgcgcct gcatcatgca gcttgcgttg
      661 aatggccgag atcgtcacga gatggcggtg ctcgtcttcc ggaatcgtga ggccggccat
      721 gctcgcgtac atgttcgaca attcacttcg ctccgtttcg ttgaggtcaa cgtacgagtc
      781 gtaaagaaat gatgccaggt cgtactgccg tagtccctgc ctcgcgtcct gaaaatcgat
      841 gagcccgtag gaaccgtcat ggcggacaag aagattgctg gactggtagt cgcggtgagt
      901 gaatacccgg ggttccctgc tcaggcgtgc ccccagccat gcgaaagctc gatcaaggat
      961 tttcgattca gcgcaggtcg gctctatttt ccagtgccgc gcgatcaacc attcgcggac
     1021 gtggaaacgg tattcaaagc gccacttttc ctcgtcgaaa tgaagctgaa aagcaggaca
     1081 gggaaacgcg tcattctgcc ggcacgtgtg aagccgcacg agtaagccaa ttgcgccctc
     1141 aagaagggcg caacggttct cctgttccga cgcgataacc tgcgtgagcg ttctggttcc
     1201 aaagtcctcg agaagggcga ttccctgatc gcgccatcgg gccagaatcg ccggtgcagg
     1261 gatactctgg ctgcggaggt actcaccgtt ggaacagaac gcgtcgaagg ttccaacctc
     1321 gactgtcgga acaaccatta cgacgtacgt tgcagccgcg tcactcgctt gcgccgccag
     1381 tgacaggcgg aaataccgtc tccccgacgc tccaccgtga atgggggaaa gctcgtgtct
     1441 ccggggtgag gaagcggcac tcaattctgg accgtatcaa gattcgatgt cgaggctaat
     1501 gggggcggaa aggggtaccg gcttgagatg cgaaaaacga acgccggcga gatccctcct
     1561 cacgcgaacg taatcctgga ccgttccagc gtccgcccag tatcctgagt gaagataccc
     1621 gtgtatgtgg cggccctccc gcaaagccgg cagatagaag gcgtcaatta tggattcctt
     1681 cctcttcagg aggtaatcga agacggcagg ttccataaca tgaattcccg caaaggtgag
     1741 tgagcggctg cattttgttc ggacaccaag ctctccccgc aaatccttca cgtgtttgct
     1801 ccgggaatcc cagacgaccg gggcgtactg actctggccc tcccgttcca ccagcaccat
     1861 cgtcgcgatg gctccgctgc gaatgtgagc ttcgaggaca ggtttgagat caatatcaca
     1921 cactacgtcg gagttcagga ggaagaatgt ttcgttgccg agaaaaccct gcgcgttgct
     1981 cagcccgccg cccgtctcga gaaggttccg ttcgcgggcg aagcgtatgt tcagccccat
     2041 ggtggagcat gccttcagcg cgctttctat ctggtctgcc agatggtaca cgttgacaac
     2101 tacgtccttc actcccgctt ccttcacgag cccgagcgga tatgcaatca gaggcttccc
     2161 gttgacaggc agaagcggct ttggaagaga tttggtgtac ggtaacagcc gcgttccgag
     2221 ccccgcggca agaatcattg ctctcatgga actgcctttt tgtccttttt acgtcacccg
     2281 tgtggttgtc ttttcctcga ccaggtccac gatgttctcg tcgatcggaa acgaaactcg
     2341 acaaccgcga acgataatct cctcgatcga aacgccgaag taattgagag cattggggtt
     2401 gaaatcgaaa tccattgtta ccgaataggt aacccacact gcccggtctt cgcggatgat
     2461 atcgattgtc tgcgttgccg ctccccttac cgtggctcgt tgaatggcct ccacttcgcc
     2521 cggttcaagc gcggaagcgt cccaggagac ctccggtttc tgaaagcacg cctgtatcgc
     2581 ggaagcaggt gctcgcggat cgagcaaatc gtactcggcg acagttgttc gaacggccac
     2641 cgcatgcatc cgggttccgc cgccttttcc ggcgtcaggt gcacgcgcct ttacagtacg
     2701 tcctgccaag gagttacctc cacctcctca tcggctgctg cggaacggta aagagcatcc
     2761 atgataacgt tagtcaccac aacctgttcc ggcggtatgg gcgacggccg accatcgcgc
     2821 acacattcca ggaagtccct cgtctccagt acgaatctgt cttcctccgg gggcaggtcc
     2881 tgcggaatca cattgaccat catcccgaac tggtcgccgt agtactccag aggtccaaga
     2941 gctacgcccc ctttttcccc gaggaaaaac gagcggccca tgctgtttcc gtgtatggcc
     3001 cacgatgttt tgaaggtgat gcagaggccg ttttcaaaac gaatccatgc ggccccgaat
     3061 tcctcgacat ttgttcggtc aacatcccag ctatgcccgc catacacctg tgcggatggg
     3121 tctttggtaa tggccataca ggtggtgccg cttacgcgct tcggcttcgg atgtccgagc
     3181 agatgaagcg ccgtgtcaag agaatagact ccgatgtcaa ccacagcgcc accgccggcg
     3241 gtctcttgtc gggcgaacga gccgctgccg atccagcggc gccgtgtgct tatcgtttcc
     3301 ccgtaataca cccgtcccaa agcgccggaa tcgattattt tctttgcggc aatcgtctgc
     3361 gggctgtacc gagaatggat tgcgatcatc aggataagcc cgctgtccct ggctgctttc
     3421 gccatcgcct tggcgtcatc gagagttgcc gccatcggtt tttcacaaag aacgtgtttg
     3481 cccgccgcaa gggcgtctac cgtgggttgc cggtgcgcct ggttgtacgt gcacacactg
     3541 accacgtcca cgtcatcccg cgccagaatt tcgcggtaat cggtcgtaaa gtaatcaaca
     3601 ccccaccggc ttgcagcctc ctccgcacgc gccccgtcga tgtccgcaac agccacgatc
     3661 tttcccagcg aggcgttagc ctggtagccg cggacgtgag aattcgaaat gcctccagtg
     3721 ccgataattc caatgccgat agacatttgc ggctccttgc tccacatgtt gtggcatggt
     3781 tccgatatcg ggaacgagtg atcggtacga agaagatacg acctcggggt cgaaccgcgc
     3841 cactggagcc agttcgaacc tctcctggcg ctgggggttt cgagaagcgc tttggcggtt
     3901 ggaacgcccc catgtcatat cgagaaatgc tcttcggctc gccggtaaat tctgtccagt
     3961 tctctctgca cctcttccgg caacgcgaac gcgtggcgag atgggagacg ccgggcttcc
     4021 tccctgagtc gctcgttgag ctcccggctt ccggaatcct gccacgcagc cagaaggcca
     4081 cgctcgaaga aacggggata ccagcacgac tctcggtacc gttgcagcgt ggaatcaagg
     4141 ccaaggaatc cccgcgacga tccttcgatg acatcttgga cacacgcatc gggatcgcac
     4201 gatgtgtcga gcccacggac aacggcttcc acgtgtgatg caatctcgca gtcgagaagc
     4261 agttgttccg cactgaatat ttcgtcgaga ctcaacgttc ccgcgcatac gaaatggcgc
     4321 gcgccgagaa gcgcccccgc cgtcatgatg ctggcttttt ccgccgcggc ctgcggcccc
     4381 ggtgttttcg ccatcgtgtg gatattggca gccgacggtt tccattgagt gccgtggaag
     4441 tatgcatcca cttctgcgga agccagttgc atcaggaggt tctccggcga accaaacact
     4501 atcgacagcg cccgcatgtc gaacggaaag atctggatct cccagcctac gtcgaggtcg
     4561 cagagctcgt gcatgatcat cgcgcttcca atcacctctg ccgccgccag agcgaaggct
     4621 tccccggtcg cctgcggtgc ggtcgctccc gccgatggca tggaaaccac ccatgtggaa
     4681 gcgatcctct tttcgaacgc cagaacggcc gcgagcgagt ccccggcgag agtgagtggg
     4741 ctgacaagat agaccggtaa gagcgtaacg ggatggccga ccgtttccgc catgtcgagc
     4801 acgtactcgc acgccgctac tgatcgagga tccggcggct gaaaaccgta ccgggtgtag
     4861 gtgaggctta ggtagtagat ggagaccggc tgcaagtccg ggggaacatc cataggcgtt
     4921 cccggagctc cggcccgcac gccctggtcc gccagaacgt ccaccgtttt cgctgcttcg
     4981 atcagagtgc tcactgtgta cggcctgatt tgccccgttc ctgcgtctcg aacgtgctgt
     5041 gaatactggc tcaccccgag tgtgatttcc tgcgtcgacg gcggtgtggc gtccatgccg
     5101 ctcacctttc cgccttttgc gcgttcctgt tccagaaacg tgcgggtgat gcgtcgatca
     5161 aagaccacac gaccgtcgtg gaagcacatt cccttttggc gaagcgtggg gacgaggtct
     5221 tcctgtacct tgatgcccac ctgctcaagg attcggtacg cagcttcttc aatctgctca
     5281 agatccgacg ggttcagcaa agagcttcgg attgccgata gtggcgtgaa tggcatagtg
     5341 tacttctcgc cccgctgcgg atggtgtagt tgccgttagg cgtttacccc cgtaaccacg
     5401 ataacacgct caatgtgtgc gtagtcacgg tggatcgaaa tccttccctc aggaaggtat
     5461 gattggcata tctg
//
LOCUS       DAOSOO010000208         5457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15072_length_5457_cov_4.957608, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000208 DAOSOO010000000
VERSION     DAOSOO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15072_length_5457_cov_4.957608"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLT86_12195"
     CDS             <1..748
                     /locus_tag="PLT86_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37414.1"
                     /translation="GTPVLHIDLGDMSENSHLSTRTGIFRLTDFNTEDYIINTKYVDQ
                     RWESYRNNGILDFSGNDRGEDVGLDGCPDAYEDGTGGCLDTPNPDYPAVTDDPNGDNW
                     SFRHTGSPYDFEHINGTQDNRQDSEIQPYPDNEDLNGNGSLDRTNSYLTYTIELDENS
                     PYAVEETEQARSGFRLYRIPLKELGIGTVDSVGGTAPAFNRITTARMWVDGVRDSALW
                     VTIASMDFVGNDWEEVKQAGEQFATTTVNT"
     gene            773..3490
                     /locus_tag="PLT86_12200"
                     /pseudo
     CDS             773..3490
                     /locus_tag="PLT86_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025707.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3540..5000
                     /locus_tag="PLT86_12205"
     CDS             3540..5000
                     /locus_tag="PLT86_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37415.1"
                     /translation="MKRVKIGWATRDVTTTEPVGIPGQFHLRISEGVLDPLTVTALVV
                     DGGDDTAIFLSADLVVIRSFLLDLVREKVARLNPEIPVLKILMNATHTHTGGDHYTSG
                     SDDFPCDLPRYSGDEYRELLATGCAEAVAEAWATRAPGGVAWGYGYAVVSYSRRVVYF
                     DDVSQRPGATRDSAAGVNGHARMYGNTNDPMFSHYEAGADHFVNLLFTFDAENRLTGA
                     IVNVPCPSQVSEHLWQLSADYWHDVRLAIRKQFGNIFILPQCAAAGDLSPHILHYKKA
                     LERRLALKGMSERQDIAERITTAFAEVYSWARNDVRTEVPVRHAVETVELSRRRITED
                     EFEEDSRMLEEFNKKQFATEGTPKERLIHDSFLSALRKRAQGVIDRYRTQDANPKLPM
                     ELHVIRIGGVAFASNRFELYMDYMHRIQARSPFEQTFVVQLAGVPGPDGGTYLPTERA
                     VWGKGYGASRYCNQVGPEGGQELVEHTLRLLNGVKD"
BASE COUNT         1132 a         1752 c         1608 g          965 t
ORIGIN      
        1 cggaactccc gtactgcaca tcgacctcgg ggatatgtca gagaattccc acctgtcgac
       61 cagaaccgga atcttccggc tgacggactt caacacggaa gactacataa tcaacacgaa
      121 gtatgttgac caacggtggg aaagttaccg gaacaacggg atacttgact tcagcggcaa
      181 cgaccggggc gaggacgttg gccttgatgg ctgcccggat gcgtatgaag acggtaccgg
      241 cggctgtctg gatacaccca acccggatta cccggccgtg accgatgacc cgaacggaga
      301 caactggtcg ttccggcaca cgggctcccc gtacgatttt gagcacatca acggaacaca
      361 ggacaaccgg caggattccg aaatccagcc gtatcccgat aacgaggacc tgaacggaaa
      421 tggctcgctt gacaggacga acagctacct cacgtatacc atcgagctcg acgaaaacag
      481 cccgtacgcg gttgaagaga cggaacaggc gcgctccggc ttccggttgt accggattcc
      541 cttgaaggaa ctcggcatcg gcaccgtgga ttcagtcgga ggaacggcgc ccgcgttcaa
      601 ccgaatcacc acggcgcgca tgtgggtaga tggcgttcgc gattcagcgc tctgggttac
      661 catcgcctcg atggacttcg tcgggaacga ctgggaagag gtgaagcagg caggcgaaca
      721 attcgcaact accacggtga acacgtagga caacaaggca tacaccccgc cgcccggcgt
      781 ggaacgagaa ctggacgcgc ttaccggcgc gctgcgacgc gagcaatcgc tcgtgatgca
      841 gatcatggaa atcgccccgg ggcagaccta ccgcgcacaa cgcgacctct acactgccgt
      901 gaatcttacc gattaccgca gactgcgcat gtacgtgcac ggccccgaaa caggcatcaa
      961 ccccacgtgg gaccatctgg ccgcgttcct gaggctcggg ctggactcga ccagctacta
     1021 tgagctgcgg atcccgcgcc tctaccccgg ctgggatgag cggaattacg ttgacgtgtt
     1081 catagactcg ctgaccaatc tcaaactgac agataccggg cagttttttt ccggacgcga
     1141 tacgatctcc accgatggaa ggcttcgggt tgtggcggca cagcgcggcg ccagcgtaac
     1201 cttgccctcg ctttcctcga ttcgcgtgct gcagatcggc attgtcaacc tgagcgacca
     1261 taccatccgt gcgccgtcca caaatcaacc catccaggtc tggttcgatg atcttcggct
     1321 cgagggcgtg cgcaatatag ccggccgggc gtaccgtgcc ggtttgaaaa tgcagatggc
     1381 tgacctggtg aatttgcagg tttcgacgaa ccggcagcag atcggctttg gaagcatcca
     1441 gaacaagcag ggaagcggca cggagacacg gtcgtacaca gtcgccgtgg accggttccg
     1501 gctggacaaa ctacttcccc ccgcctggcg ggtgtccgtt ccggtcaacc tgaattactc
     1561 cgtcaacacg aatgtccctc gtctcaaacg gggttcggac atcgaactga ttgatcagcg
     1621 cgataaggac gtggagcgcg cgcggacggc gaccataaat gcgtccacgt ccttcgtgaa
     1681 gagctcccaa tcgcgcaatc cggccatcgg gctcactctc gatcgtgtgg gggcgggtgt
     1741 cacgtacagc ctttcgcacg ggctcacgcc gggtcttact tcccgggata gctctcactc
     1801 gatgagctac acggggaggt tcaattacga cctgacgccg cggcgacctc atcagctccg
     1861 catcctggga tgggtaccgg accgccttcc cggcaaagtg gaggagacgg aattcgccta
     1921 cctgcccacc agcctccggt tcgacgccac cacgacgttc cgcgctgact catcatggtc
     1981 gaagcagaag gttctcggaa gggatacgac gctggcgcgg gggcaacggg tgttcacgat
     2041 ttcggaagca taccgtatcg ggttccagcc cttccgctcg ctgacaacgg cgtacgacat
     2101 gtcgctgaac cgcgatctcg ccgatgcgct ggaggaggat accggtcccg gagcagtaat
     2161 gcagtcgctg gcgaaaaaca ttttccgaaa ccgggaaata cgccggtccc agaacacgag
     2221 catcaactac tccccgacgt ggccctggtg gttgggccaa tcctatacct acacgaacca
     2281 gtacaacgac aacagcgacc cgcgcaccac ctccggaagc acctccggcg cacggcagta
     2341 tgaggtcacc aactcgcggg ggtacggcat atcgagcatg acgttcaaag tgcgcgatat
     2401 cctcacccgt ttttccggtg aaaccggagg aacgggcggc ggcatgcggc gtggcggaat
     2461 ggcaccggcc gctccacggc ggccggtttc gacggacacc acgggaggct tctctctgct
     2521 ccgccccctg cgggcaacgg ccggtttcgg gggcaatcat ctggaaaatg tgatcggccg
     2581 catcacattc aacgagcgat tcatgggcac ccagattccc gattcgctcc gtcccgatct
     2641 caaataccag atactgggaa tcggcaggca cccggtgttg cccggtgtgt cgtacaccat
     2701 ccgcccgcag aactccatca gtcaggatct gggctgggat gtatcaagtg gtctgcagtt
     2761 gccgttgagg atgcggctgc aggcgaagta cgcgttccgc acaaccaaaa ccatgggtgc
     2821 gcgcgatacc acgcgatccc gcgatatcac cttcccggac gtcgggtatt catgggacgg
     2881 cctcgagtcg ttgcctcttc tccgcatcgt caccacgcgc agcgaggtcc agtcctcgtt
     2941 cacgcgccgg atcaacacaa gctggacgag aacaggcggg ctccccgagc gcctcgaccg
     3001 ccgttccacc tcgtatgtgc tgagcccgct cttctcgtgg agcatcctct ggaagaacag
     3061 cattcggtcc acgatcagga gcacatggga tcagacgctc gacgagcggt attccgcggc
     3121 gggaatctct tcgacccaga caaccaactg gggcgttacg ctcaacacga cgtacgagct
     3181 tcaaacgtcg cgcggaatct caaaaccgtg gggcggcaag tggcaacttg cgggcaacat
     3241 caacctgtcg cttgacggaa gctactccgg cagctacgcc gtcgggctgg gtgccaaccc
     3301 ctccacgggt ggcgaccttg tgcaggggca tacacgcacg tggagcatca aaccacaggc
     3361 acagtaccag ttcagccgga cctttaccgg gcaggcgcag atagaagtcg gctccatgac
     3421 ggacctgcgc aaccactaca cgatgaaaac ccgtgccgtc atgattaccg gagaactccg
     3481 attcaactga gcgggcgatc aacaccgcag acggccgcgc tctgacaagg ggacacagga
     3541 tgaaacgcgt gaaaatcggc tgggcaacgc gcgacgtgac taccaccgaa ccggtgggca
     3601 ttccggggca atttcacctg cgcatctcgg agggcgtcct cgacccgctc accgtcaccg
     3661 cactggtggt tgatggtggg gacgacaccg ccattttcct ttcggccgat ctcgtggtaa
     3721 tacgttcatt tctgctcgac ctcgtacggg aaaaagtagc gcgcctcaac cccgaaatcc
     3781 cggtgctcaa aatcctgatg aacgccaccc atacgcacac cggcggtgac cattacacca
     3841 gcgggtccga tgacttcccg tgcgacctgc cccgctattc cggcgacgaa taccgcgaac
     3901 tcctcgcgac cggttgcgcg gaggcagtgg ccgaggcatg ggcgacgcgc gcccccggtg
     3961 gggtagcgtg gggatacggc tacgcggtcg tctcttacag ccgccgggtg gtctatttcg
     4021 acgacgtttc acagcgcccc ggcgcgacca gggattccgc cgccggtgtg aacggccatg
     4081 caaggatgta cggcaacacc aacgatccga tgttcagcca ttacgaggcc ggcgcggacc
     4141 acttcgtcaa tctgctcttc acgttcgacg cggaaaatcg gctcaccggt gccatcgtca
     4201 acgtgccgtg cccgtcgcag gtgtcggaac acctctggca gctttccgcc gattactggc
     4261 acgacgtccg cctcgccatc cgcaagcagt tcggcaatat cttcatcctt ccccagtgcg
     4321 ccgctgcggg ggatctttcc ccgcacatcc tccattacaa gaaggcgctt gaacgtcgtc
     4381 tcgcgctgaa gggcatgtcc gaacggcagg acattgccga acggatcacc accgccttcg
     4441 ccgaggtcta ctcgtgggcg cggaacgatg tccgtaccga ggttccggtg cgccacgccg
     4501 tcgaaaccgt ggagctttcg cggcggagga tcacggagga tgagttcgaa gaagacagcc
     4561 ggatgctcga ggaattcaac aagaagcagt tcgccacgga aggcaccccg aaggagcgcc
     4621 tgatccacga ttctttcctg tcagctctgc gcaaacgcgc gcagggagtc attgaccgtt
     4681 accgcacgca ggacgccaac ccgaaactgc ccatggagtt gcacgtcatc cgaatcggtg
     4741 gcgtggcgtt tgcctcgaac cggttcgagc tctacatgga ctacatgcac cggattcagg
     4801 cacgcagccc gttcgagcag acgttcgtgg tgcagcttgc cggcgtgccg gggcccgacg
     4861 ggggcaccta ccttccgacg gagcgcgcgg tgtggggcaa gggatacggc gcctcgcgct
     4921 actgcaatca ggtcggcccc gagggcgggc aggagcttgt ggagcacacc ctgcggctgc
     4981 tgaacggggt gaaggactga ttgcccgcgc ggacccacgg tcggcaagtg gcgggccacc
     5041 ctgcgggcgg caggtgtatc gaaaacacta attcccgccc gaccgtagca cgggcgtccc
     5101 gcccgtgcgc gcaaattgcc ccattcagaa actgcacggg caggatgccc gtgctacggc
     5161 tattcttcta cgcgcaacac cgccattctg agcagcgtcc ccagcgcgga cgattggcga
     5221 agtgtctcct ccgcccggct cctcgataca gcgctgcgtc cgcggcgcgg acgttcgcgc
     5281 cactcggaga ccgggcacac ccgcacgcgg tggggcaggc attcctgcct gccatatgct
     5341 tctttttact tccccttctt ctccaccgcc acatgctcgc tgctcaccac gcatccgcgt
     5401 tcgtcaacca gatttatgta atgccccgtc gcgccggcgg gcaccttcgc cgaggcc
//
LOCUS       DAOSOO010000209         5431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15161_length_5431_cov_6.858445, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000209 DAOSOO010000000
VERSION     DAOSOO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15161_length_5431_cov_6.858445"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..945
                     /locus_tag="PLT86_12210"
     CDS             223..945
                     /locus_tag="PLT86_12210"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT37416.1"
                     /translation="MSLIIIIGRGHGGTRAMSQTLVDSGVYMGEPLNDSYDLVPPEAM
                     YDACRVMARYVKWHGGFRWDWSQLHSMDIPEEFTGLIQTYLKSVLESPAPRKGWKIPE
                     TTLVFPWIVRMFPEAKYIFWIRDPRDSILGGHVTDDLSDFNIPYPLTEDERRRRAISW
                     KYQYDLVKATPKPAHWIEVRFEDFVLKQEETLPRLEDFLGMKLAKIPVRPEAVGRWKT
                     AEGPHTFDFFEPALVEYGYEKG"
     gene            complement(1093..2217)
                     /locus_tag="PLT86_12215"
     CDS             complement(1093..2217)
                     /locus_tag="PLT86_12215"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT37417.1"
                     /translation="MVLRRNGHPLFTLGMYERPRNDDEWAAWSKAGVNLVCCSTREHL
                     DAAHRWGMFGWVPVPMILAPEDNGAQLAERIRSLADHPALAVWEAPDEAIWHAHRQPT
                     NWATKRLWSLPPEDQRVVQERLDAVVHGLARGSTLVRSIDPGRPLWLNESGANQLTLA
                     RCVSSLDIVSVDAYPVPPRIEKPVHHIGLDIDRFRAVAPNKEVWMVEQAFSWNNLYPE
                     RKKVEAPVYPTLEESRFMAWQAILHGATGLLWWGSSYEDRPAEIVDTVMQVVSEISQL
                     NEYLIGGEVFSVRVVADTRAYPATALGIGHICRRAGDGTLLVLANEDPFDQEAIITGV
                     DWLDVSKMRPVCEPSASLTRIDEGWITPMKGYEVRLYVSG"
     gene            2494..2811
                     /locus_tag="PLT86_12220"
     CDS             2494..2811
                     /locus_tag="PLT86_12220"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT37418.1"
                     /translation="MVSMEQIEEFGRRIGEQFKPERVILFGSYAYGTPTPDSDVDLLV
                     IMPCTKKPAHHAAEIRLALSAGFPLDLVVREPETLEARVALGDPFLSEIKEKGKVLYE
                     SAG"
     gene            2795..3139
                     /locus_tag="PLT86_12225"
     CDS             2795..3139
                     /locus_tag="PLT86_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT37419.1"
                     /translation="MNPLVEEWIETGEGDFATARRELRAKKHPNYNAACFHAQQCAEK
                     YLKARLLMAGTRFPKTHDLEALVKLLLPHEPGLLELLPMAVSLTSLGVDVRYPGHPVD
                     KATAAEALASAT"
     gene            complement(3473..3931)
                     /gene="mscL"
                     /locus_tag="PLT86_12230"
     CDS             complement(3473..3931)
                     /gene="mscL"
                     /locus_tag="PLT86_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT37420.1"
                     /translation="MFQEFKKFAMRGNVLDMAIGIIIGGAFGTIVKSLVDDVIMPPIG
                     LLLGGVDFSNFFVVLKDGVEKAGPYAALADAKAAGAVTIAYGAFINSVISFLIVAFAV
                     FLLVKSFNAMQRKQEAAPAAEPTTKECPFCFSAIPIKATRCPHCTSEIRT"
     gene            complement(4016..4864)
                     /locus_tag="PLT86_12235"
     CDS             complement(4016..4864)
                     /locus_tag="PLT86_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT37421.1"
                     /translation="MVIGSDWHIHSHHSCDQAALRMADLIREAAPNGVTDFGITDHIH
                     TPFNRPDIVASRLEFEGVRDNPRFHFGVEASCVSRWEIDEIARGGHENPVYGLRTGGP
                     PGCELAIALTAEDITELGIEYVIGGTHWPMYVPLERHAVIHDYHRQNMFLAMHPLVDI
                     VAHPWWWHGGWEDAQHNLSAEPWFDDFTCIPESMHDEFAAAAIEHGKKVEINLNALLL
                     NPHYPEGFKLQYLVYLAKLKDRGVQISLGSDCHAPHYTVGFERAARMLESVGFVEADF
                     WVLEPR"
     gene            complement(4880..>5431)
                     /locus_tag="PLT86_12240"
     CDS             complement(4880..>5431)
                     /locus_tag="PLT86_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37422.1"
                     /translation="GEFLVFCGVVGLGRLLAEGDHSLLPRIRRYASDFRWRTREAVAM
                     ALQRWGRSDMAALLAEMEHWAAGNRLEQRSAAAAVAEPALLRNPADAGRALSVLDTIT
                     RTIPGAPDRRTEDFNTLRQGLGYCWSVVVAAVPEAGKAAMERWFAADSPDVRWIMREN
                     LKKNRLAQMDADWVTYWRDRLKT"
BASE COUNT         1066 a         1621 c         1715 g         1029 t
ORIGIN      
        1 gagtcccgta ttccgttgac atcgtcttca cgccacggtt acggagttct gcgccgtttc
       61 gccccgcgag ttgaccacct gactgattcg aggtccaccc cgcacgttcg acgggtttcc
      121 cccgcggcac gcgcccgttt cggaaaccac cgtacgatct gacaacttca tcccgccatt
      181 tcccagaaaa tgggctccag tggaacagga ggaaagcgct tcatgtccct gatcatcatc
      241 atcggtcgcg gccacggtgg cacgcgggcc atgtcgcaga cgcttgtaga cagcggcgta
      301 tacatgggcg agccgctgaa cgattcctac gacctcgtgc cgcccgaagc gatgtacgac
      361 gcctgccgcg tgatggctcg atacgtcaaa tggcacggcg ggttcaggtg ggactggtcc
      421 cagctgcact cgatggacat cccggaggaa ttcaccgggt tgatccagac gtacctgaag
      481 tcggttctgg aaagcccggc accgcgtaag ggctggaaaa tccccgaaac gacactggtg
      541 ttcccgtgga tcgtgcggat gttccccgaa gcgaagtaca ttttctggat tcgtgacccg
      601 cgcgacagca ttctcggcgg acacgttacc gacgaccttt ccgatttcaa cattccgtat
      661 cccctcacgg aagatgaacg ccgccgccgg gcgatttcgt ggaagtacca gtacgatctg
      721 gtgaaggcga caccgaaacc ggcgcactgg atcgaggtgc ggttcgaaga cttcgtgctg
      781 aagcaggagg agacacttcc gcggctggag gatttcctcg ggatgaagct ggcaaagatt
      841 ccggtccgcc cggaggcggt gggccgctgg aaaacggctg aagggccgca cacgttcgat
      901 ttcttcgagc cggcgctggt ggaatacggg tatgagaagg ggtaaataac accctgcgcc
      961 agaacgcgca gacgccctca ccccgtcgtg cggcagtaat gggtcgtacg ggacggatgg
     1021 gacacatcgg cggggcgggg acccgacgga gcgggtccct ccagaggttg cagcgccccg
     1081 gcggcagggg cgttacccgc tcacgtacaa ccgcacctcg taccccttca tgggggtgat
     1141 ccagccttca tcaatgcgcg taagtgacgc ggaaggctcg cagaccgggc gcatcttgct
     1201 cacatcgagc cagtccaccc cggtgatgat ggcctcctga tcaaacggat cctcgttggc
     1261 aagcaccaaa agggtgccgt ccccggcgcg gcggcagatg tgtccgatcc cgagcgccgt
     1321 cgcggggtag gcgcgagtat cggccaccac acgcacggag aacacttccc cgccaatgag
     1381 gtactcattc aactggctga tttccgacac gacctgcatc accgtgtcta cgatctccgc
     1441 gggacggtct tcgtacgatg atccccacca gaggagcccg gtcgcgccgt gcagaattgc
     1501 ctgccacgcc atgaagcggc tctcttccag cgtcgggtag acgggcgctt ccaccttttt
     1561 gcgctccggg tagagattgt tccacgagaa ggcctgctcc accatccaga cctctttgtt
     1621 cggcgcgacc gcccggaacc ggtcgatatc gagaccgatg tggtgaaccg gcttttcgat
     1681 gcgcggcggg acagggtagg cgtccaccga gacgatgtcg agggaggaca cgcaccgggc
     1741 gagggtgagc tggttcgcgc cggattcgtt cagccagagc ggacggcccg gatcgattga
     1801 ccgcaccagc gtggagcccc gcgcaaggcc gtgcacgacc gcatccaggc gttcctgaac
     1861 cactctctga tcctcgggtg ggagggacca gaggcgcttg gtggcccagt tggtaggctg
     1921 ccggtgggcg tgccagatgg cttcgtcggg cgcttcccat accgcgaggg cggggtggtc
     1981 ggcaagggag cgaatgcgtt cggcgagttg cgcgccgttg tcctccgggg cgagtatcat
     2041 cggcacgggc acccagccga acatgcccca tctgtgcgcg gcgtcgaggt gttcccgcgt
     2101 gctgcagcag acgagattga caccggcctt gctccacgcg gcccattcgt cgtcgttgcg
     2161 cgggcgttcg tacatgccga gggtgaaaag cgggtgaccg ttgcgtcgca gaaccatggg
     2221 tgaccctccc tgaagtggcg gtttcacgga tgtgtgttga tggtagcacg gtgccgttgt
     2281 tgccgcaacg cctgcacggg agtgacgcgg catggaatga cggcgaggca gtcgtcaaga
     2341 caacaggatg ccgtggccag cgtgacggta cggcctgaga tgaccgtggc gcgcgggaac
     2401 atgccctcgg cacgggagag cgcgacgttc gcccgtgcca ccgatgacgt gctttcgtat
     2461 actattggtg acacagaaaa agagggggtc gccatggtct ccatggagca gattgaggag
     2521 ttcggccgca ggatcgggga gcagttcaag cctgaacgcg tgattctgtt cggttcgtac
     2581 gcgtacggaa cgccgacgcc ggattccgac gtggatttgc ttgtgatcat gccgtgcacg
     2641 aaaaagcctg cgcaccatgc cgcggagata cggctcgcat tgtctgcggg gtttccgctg
     2701 gatctggtcg tccgtgaacc agaaacactg gaggcgcggg tagcgctcgg tgatcctttt
     2761 ctctcggaga tcaaggaaaa ggggaaggtg ctttatgaat ccgctggttg aggagtggat
     2821 agagaccggg gaaggggatt tcgcgacggc acggcgggaa ctgagggcaa aaaaacatcc
     2881 caactacaat gcagcgtgtt tccacgccca gcaatgcgcg gagaaatacc tcaaggcccg
     2941 attgctcatg gccggaaccc gcttcccgaa aacacacgat ctcgaagctc ttgtgaaact
     3001 gctccttcct cacgaaccgg gactgctgga actgctgccg atggcggttt cgctgacctc
     3061 cctgggggtc gacgtgcgct accccggcca cccggtggac aaagcaaccg cggcggaagc
     3121 cctggctagt gcaacgtgat acgcgccgca gtaaggcgat cgctggggct gaatgactga
     3181 acgaatcctc gatggagccg tgactgcgac aaatcaggca actcgcgccc cgcaaacgag
     3241 ctgattcgcc tggcttgtag aaatggcggt tcggttcaag gaggtgttcg ggcgacgaca
     3301 ggaacgcagc gaagacgcag caggagattc ttcacttcat tcagaatgac ggcgaggcag
     3361 cgcgttccga tggttgagaa agagagcggc atcaacaaaa cagggggagc gcagggcggt
     3421 attgtgccgc ctgacagcgc tccccttgtt gttccggttg ttgccggaga gatcaggttc
     3481 tgatttccga tgtgcagtgc gggcagcggg tggccttgat cggaatggcg gagaagcaga
     3541 acgggcattc cttggttgtc ggctccgcgg ccggtgcggc ctcttgcttg cgctgcatgg
     3601 cattgaagct tttcacgagc aggaataccg cgaacgcgac gatgaggaag ctgatcacgg
     3661 agttgataaa cgccccgtag gcgatcgtca cggcgccggc ggctttcgca tcggcgaggg
     3721 cggcgtaggg acccgccttt tccaccccgt ctttgaggac gacgaagaaa ttcgagaaat
     3781 cgacgccgcc gaggagaagc ccgatcggag gcatgatcac gtcatccacg aggcttttga
     3841 cgatagttcc gaacgcgcca ccgatgatga taccgatggc catgtcgagc acgttgccgc
     3901 gcatcgcgaa ctttttgaac tcctgaaaca ttgcgtccct ccttgtgcag tgccctccgc
     3961 acggccgttc ctgttgctgc gattcagcat ggccgaccgg ccgcgcaccc gtgtctcaac
     4021 gcggttccag aacccagaaa tccgcttcca caaacccgac cgattcgagc atgcgcgccg
     4081 cccgctcgaa cccgaccgtg taatgcggcg cgtgacaatc cgacccgagt gaaatctgca
     4141 cgccacgatc cttgagcttt gccagataca cgagatactg gagcttgaac ccttccgggt
     4201 aatggggatt caacagcaac gcgttcaggt tgatttcgac cttcttcccg tgttcgatgg
     4261 ccgcagcggc gaactcgtcg tgcatggatt ctggaataca ggtaaagtcg tcgaaccagg
     4321 gctccgcgga caaattgtgc tgtgcatctt cccagccgcc gtgccaccac cacggatgcg
     4381 caacgatatc aacaagcggg tgcatggcca ggaacatgtt ctggcgatgg tagtcgtgga
     4441 tcaccgcgtg ccgttccagt gggacataca tcggccagtg cgtgccgccg atgacgtatt
     4501 caatgccgag ttccgtgatg tcttccgcag tgagcgcgat ggcgagttcg caccccggag
     4561 gaccgcctgt ccgcaggccg tagacgggat tttcgtgacc tccgcgggcg atttcatcga
     4621 tctcccatcg ggaaacacaa cttgcctcca ccccgaagtg gaatcgcggg ttatcgcgga
     4681 cgccttcaaa ctcgaggcgc gaggctacaa tatcggggcg attgaacggt gtgtggatgt
     4741 gatccgtgat gccgaaatcg gtaacgccgt ttggcgctgc ttcgcggatg aggtccgcca
     4801 tcctgagagc ggcctgatcg cacgagtggt gggagtgaat gtgccagtcc gagccgataa
     4861 ccatgtgttc ctcatcacat tacgtcttca gtctgtccct ccagtaggtt acccaatccg
     4921 catccatttg tgcaagtctg ttcttcttca ggttttccct cattatccat cttacgtccg
     4981 ggctatccgc ggcgaaccac ctttccatgg cggcttttcc cgcctcaggc acggccgcga
     5041 cgaccacgct ccagcagtac ccgagcccct gccgaagcgt gttgaagtct tccgtacggc
     5101 ggtcgggtgc tcccgggatg gtgcgggtga tggtatcgag aacggaaagt gcccgaccgg
     5161 catcggcggg gttcctgagg agtgccggtt ccgccactgc cgctgcagcc gaacgttgct
     5221 cgagccggtt tcctgcggcc cagtgctcca tctccgcaag aagagccgcc atgtcggatc
     5281 tgccccagcg ctggagcgcc atggcaactg cttcgcgggt tcgccagcgg aaatcggacg
     5341 cgtagcggcg aatgcgtgga aggagcgaat gatcgccctc cgccagcagg cgccccagac
     5401 cgacaacacc acagaagaca aggaactcgc c
//
LOCUS       DAOSOO010000210         5429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15171_length_5429_cov_5.958318, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000210 DAOSOO010000000
VERSION     DAOSOO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15171_length_5429_cov_5.958318"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLT86_12245"
     CDS             complement(<1..710)
                     /locus_tag="PLT86_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT37423.1"
                     /translation="MTQGKISVATCQFAVGPNPRRNGAQIRRQMVQAKDAGAEVVHFP
                     EAALSGYGGADVKRWEDYDWNALREETERVMELAARLGVWVILGSSHRLSGDHLPHNS
                     LYAIDPSGRIVERYDKCFCTGSDLKMYSCGDHLSVITINGVRCGLLICYDVRFPEIYR
                     AYKRENVACVFHSFYNARADGPSIHTTVMRPTLQGHAGINYVWISATNSSAYYGSWPS
                     VFVRCDGTILGSLRFHRAG"
     gene            complement(727..1806)
                     /locus_tag="PLT86_12250"
     CDS             complement(727..1806)
                     /locus_tag="PLT86_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37424.1"
                     /translation="MLRTPSASERVQMVDPETGIRITQLTSYPTPSVHLLYYWPSVTP
                     DNKRIVFLGQRSVARRAPWDLFRCDTDGLGLCQLTDYEQVDRSDGLPVPPAACMTLDG
                     TKLYLTRDAVLCELDVETGTMRDLVALDRFCRERSTFSHVRIGASGRIFLSQSFYAPY
                     AVRVNLATGEVAEIDLGGSLFACSQDEQRLLVGRNRYSAFWSLDEDGGDPRFHCENIF
                     AHTTTMGRLPGIQGPGRPPERCIWLAHEGKPAERLVEGPYFWHSAASFDGEWIVSDTN
                     WPDEGIKLVHVPTRHWRTLCHAHATQGHPQWQHCHPALSQDGRIAVFDSDRTGVSQVY
                     VAHVSEEFRERVKAGELDRPRDKWI"
     gene            complement(1932..2882)
                     /locus_tag="PLT86_12255"
     CDS             complement(1932..2882)
                     /locus_tag="PLT86_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37425.1"
                     /translation="MSSGELFRRAEEARRFLAPCGVCPRACGVDRLAGKTGVCGIGAK
                     ATVASALAHHGEEPGLSGARGAGTIFFGGCNLRCIFCQNHQISQTSHSPEYGPEEIAA
                     MMVRLQGEGCHNIDLVSPSHVAPQVLEAIARAAEQGLSIPILYNSNGYDSLDMLRLFD
                     GVADIYLPDLKYGDDSTGTWASSVDNYVEHAHAALKEMYRQVGDVEFDNNGIVRRGLI
                     VRILVLPDDLARVYDSLVFLAEEISTRVWLSIMSQYYPTHRAVGHPSLGRSITPKEYE
                     TVLEWVDRLGFENFYAQGMDSVEVCRPDFEKDGVFDFGEA"
     assembly_gap    2897..2996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2997..4451)
                     /gene="gcvPB"
                     /locus_tag="PLT86_12260"
     CDS             complement(2997..4451)
                     /gene="gcvPB"
                     /locus_tag="PLT86_12260"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOT37426.1"
                     /translation="MPVPLIFDVSKPGRVGHSLPACAVPAKPTESLIPAKFLRSAPPR
                     LPEVSEGEAVRHFVNLSALNHHIDRGMYPLGSCTMKYNPKVNEVAARLSGFAEMHPLA
                     PPQTTQGLLRLIHELERMLCEITGFAGITLQPPAGAASELTGLLVMRQYHRKKGNRKT
                     HVLLPDSAHGTNPASATFAGYEARQLASTEDGRLSLDALRAAVNDETAGLMITNPSTL
                     GLFETHIREIADIIHGVDGLVYMDGANMNALLGIAKPAATGVDMCHLNLHKTFSTPHG
                     GGGPGGGCLAVREDLEPFLPVPRVEHGEGDYRLAWERPDTIGNVHAFCGNVGVMIRAY
                     CYIRMMGGEGLERVSRTAVINANYLYALVKDLFDTPYQGPYMHEFVVSGDRQKARGVR
                     TLDIAKRLLDFGVYAPTVYFPLIVSEAMMIEPTETESKQAIEEYAALLRQIAREVEEE
                     PETVLKAPHTTPIGRVDEVRAARQPDLRYGFQGA"
     gene            complement(4537..5301)
                     /locus_tag="PLT86_12265"
     CDS             complement(4537..5301)
                     /locus_tag="PLT86_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37427.1"
                     /translation="MVAHILEYSAIIGTLVLMEGLLAADNALVLAVLVSRLPDRNEQK
                     KALLYGIIGAFVFRALSLVSAYWLIRMWEFKALGAAYLIFMTARYFWSGEYRKDRHAE
                     PRPFPAAKETEREGGEPAGARARFSRRFWPTVAMVELTDLAFSVDQIVAAVGLSSHLA
                     VVYTGGMIGIIAMRFAAGGFLLLIERHPKLAYTGHILPAWIGLKLAMETLSNHPFDWH
                     VKMPAWVFWSGMAALVAWGLLSRGRTDAASHEIQTK"
BASE COUNT          975 a         1627 c         1711 g         1016 t
ORIGIN      
        1 ccggcgcggt ggaaacgcag cgagccgagg attgttccgt cgcaccggac gaacacgctg
       61 ggccaactgc cgtaatacgc cgacgagttc gtcgcgctga tccacacgta gttgatgccc
      121 gcatgtccct gaagcgtcgg ccgcatgacg gtcgtgtgga tgctgggtcc gtcggcccgg
      181 gcgttgtaga aggagtggaa gacgcaggcc acgttctccc gcttgtacgc ccggtagatc
      241 tccgggaatc ggacgtcata acagatgagc agcccgcatc gcacgccgtt gatggtgatg
      301 accgagaggt ggtcaccgca ggagtacatc ttcaggtcgc tccccgtgca gaagcacttg
      361 tcatatcgct cgacaatacg ccctgacggg tcgatggcgt agagcgagtt gtgggggagg
      421 tggtcgccgg agaggcggtg ggaactgccg aggatcaccc acaccccgag gcgcgccgcg
      481 agttccatca cgcgctcggt ctcttcccgg agggcgttcc agtcgtagtc ctcccagcgc
      541 ttcacatcgg cgccaccgta tccgctcaac gcggcctcgg ggaagtgcac gacttcggcg
      601 ccggcgtctt tcgcctgaac catctggcgg cggatttgcg cgccgttgcg ccgggggttc
      661 ggccctaccg caaactggca cgtcgcaacg cttatttttc cctgtgtcac cggattggaa
      721 cctccatcaa atccatttgt ctcgggggcg atccagttcg cccgccttta cgcgttcacg
      781 gaattcctcg gacacgtgcg cgacgtacac ctgagagacg cccgtcctgt ccgaatcgaa
      841 caccgcgatg cgcccgtcct ggctcagagc agggtggcag tgctgccact gcgggtgccc
      901 ctgggtggcg tgagcatggc agagggttcg ccagtgccgc gtcggcacat gcacgagctt
      961 gatgccttcg tcgggccagt tcgtgtcaga gacgatccat tccccgtcga acgatgccgc
     1021 agaatgccag aagtagggcc cttcgacgag ccgctccgcc ggtttccctt cgtgcgcgag
     1081 ccagatgcac cgttcaggag gcctgcccgg gccctgaatg cccggcagtc gtcccatggt
     1141 ggtggtgtgg gcgaagatgt tctcgcaatg aaaccgcggg tcgccaccgt cctcgtcgag
     1201 gctccagaag gcgctgtacc tgttccgtcc caccaggagc cgctgttcgt cctgggaaca
     1261 ggcgaacagc gatccgccaa ggtctatttc ggcgacttcc ccggtcgcaa gattaacgcg
     1321 gaccgcgtac ggcgcataaa acgactgcga gaggaagatg cgcccgctcg cgccgatccg
     1381 aacgtgcgaa aacgtgcttc gttcgcggca gaaccggtcg agcgcaacaa gatcccgcat
     1441 cgtgccggtc tcgacgtcga gttcgcagag caccgcgtcg cgcgtcaggt acagctttgt
     1501 gccgtccagc gtcatacagg cggccggcgg caccggcagc ccgtccgacc ggtcgacttg
     1561 ttcgtaatcg gttagctggc agagacccag cccgtcggtg tcgcagcgga agagatccca
     1621 cggcgcgcgg cgtgcgaccg atcgctggcc gaggaacacg atgcgcttgt tatccggtgt
     1681 gacgcttggc cagtagtaga ggagatggac gctcggcgtc gggtagctcg tgagctgggt
     1741 tatcctgatg cccgtttccg ggtccaccat ctggacgcgt tcgcttgcac tgggcgttcg
     1801 gagcatggat gaggttcctt cgaagcaggg cgcgggaatc cctcggcgcc gcaaccgtga
     1861 ggggagtgct gcggcaggcc gactatcgtg ggtaaataca atgagcagga ggcttccgaa
     1921 ccaacaggcg cttacgcctc cccgaaatcg aacacgccat ctttctcaaa atcggggcgg
     1981 cacacctcca cgctgtccat cccctgcgcg tagaagttct cgaatccgag gcggtcaacc
     2041 cactcaagca cggtttcgta ctccttcggc gtgatcgagc gcccgaggct cggatgtccc
     2101 acggcgcggt gggtggggta atactgcgac atgatgctca gccacacgcg ggtggagatc
     2161 tcttccgcga ggaacacgag ggaatcgtac acccgggcga ggtcatcagg cagcacgaga
     2221 atccggacga tcaggccgcg ccggacgatg ccgttgttgt caaactccac atccccgacc
     2281 tgccggtaca tctccttcag cgcggcgtgg gcgtgttcga cgtagttatc gacgctgctt
     2341 gcccacgttc ctgtcgagtc atcgccgtat ttcaggtcgg gcaggtagat atcggccacg
     2401 ccgtcgaaca ggcggagcat atcaaggctg tcgtacccgt tggagttgta gagaatcggg
     2461 atcgaaagcc cctgctcggc ggcgcgggca atcgcctcaa gtacctgcgg cgccacatgc
     2521 gagggggaga cgaggtcgat gttgtggcag ccctcgccct gcagccgcac catcatcgcg
     2581 gcgatctcct cagggccata ttccggcgaa tgactggtct ggctgatctg gtggttctgg
     2641 cagaagatac agcgcaggtt gcatccgccg aagaaaatgg tgccggcgcc gcgcgcgccg
     2701 ctgagcccgg gctcctcgcc gtggtgggcg agcgcgctgg ccaccgttgc cttcgccccg
     2761 attccgcaca ccccggtttt tcccgccagt cggtctacgc cgcaggcgcg ggggcatacg
     2821 ccgcaggggg caaggaaccg gcgggcttcc tccgcacggc ggaagagttc gccagaggac
     2881 agatgggtct tataggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcac
     3001 gcaccctgaa acccataccg caaatcaggc tggcgcgcgg cgcgaacctc atccacgcgg
     3061 ccgatgggcg tcgtgtgagg tgccttgaga accgtctccg gctcctcctc cacttcgcgc
     3121 gcaatctggc ggaggagtgc tgcgtactcc tcgatggcct gtttcgattc tgtctccgtg
     3181 ggttcgatca tcatggcttc gctgacgatg aggggaaagt agaccgtcgg cgcgtacacg
     3241 ccgaagtcga ggagccgttt cgcaatgtcg agcgtcctca cgccgcgtgc tttctgccgg
     3301 tcgccggaga cgacgaattc gtgcatgtag gggccctggt aaggtgtatc gaaaaggtct
     3361 ttcaccagcg catacaggta attggcgttg atgacggccg tgcgcgagac gcgttcgagc
     3421 ccttcgccgc ccatcatccg gatgtagcag tacgcgcgga tcatgacgcc gacgttgccg
     3481 cagaacgcgt gcacattccc tatggtgtcc ggccgctccc acgcaagacg gtagtctccc
     3541 tcgccgtgtt ccaccctggg tacgggaagg aagggttcca ggtcttctcg cacggcaagg
     3601 catccgccgc ccggtccgcc gcctccgtgc ggtgtggaga aggttttgtg caggttgagg
     3661 tggcacatat ccacgcccgt cgccgcaggt ttggcgattc caagcagcgc gttcatgttc
     3721 gcaccgtcca tgtagacgag tccgtccacc ccgtggatga tgtcggcgat ctcgcggata
     3781 tgggtttcaa agagtcccag cgtgctgggg ttggtgatca tgaggccggc cgtttcgtca
     3841 ttcacggccg cgcgcagagc gtcgagtgaa agccgaccgt cttcggtgct ggcaagttga
     3901 cgggcctcgt acccggcgaa ggtggcggat gcggggttcg tgccgtgggc ggaatccggc
     3961 agcaggacgt gcgttttccg gtttcctttc ttccgatggt actgccgcat caccagaagt
     4021 ccggtgagtt cgcttgccgc gccggcaggg ggctgaagcg tgattccggc aaaccccgtg
     4081 atttcgcaca acatgcgctc cagttcatga atcagccgca ggagtccctg cgttgtctgc
     4141 ggaggggcga gcgggtgcat ttccgcgaaa cccgaaaggc gggcggccac ctcgttcact
     4201 ttcgggttgt acttcatcgt gcacgagccg agaggataca ttccgcggtc aatgtggtgg
     4261 ttaagcgcag agagattcac gaaatgccgc actgcctccc cttcgcttac ttcgggcagg
     4321 cgtggcggtg ccgaccgcag gaatttggcc gggataaggg attccgtcgg tttcgcgggg
     4381 accgcgcagg cagggagact gtgtccgacc cggccgggtt tgctcacgtc gaaaatcagt
     4441 ggcacaggca tgaaagaacg actccttgat cgcgaacgag tggaaaaaag tcttccttaa
     4501 gcgtacgaat gtgatgcgga agccgcaaga aacggttcac ttcgtttgga tttcgtggct
     4561 cgcggcgtca gtcctgccgc ggctcagcaa tccccacgcg acgagggccg ccatcccgga
     4621 ccagaatacc catgccggca ttttcacgtg ccagtcgaag gggtggttgc tcagcgtttc
     4681 catcgcgagt ttcagaccga tccacgcagg aaggatgtga ccggtgtatg cgagcttcgg
     4741 gtgccgctcg atgagaagca gaaatccccc cgcggcgaac cgcattgcaa tgattccaat
     4801 cattccgccc gtgtagacca ctgcgaggtg actggaaagc cccaccgcag ccacaatctg
     4861 atcaacggaa aacgcgagat ccgtgagttc gaccatggcg acagtcggcc agaaccggcg
     4921 actgaaacgg gcccgtgcgc ccgccggttc tcctccctcg cgttcggttt cttttgccgc
     4981 gggaaagggt cgcggttccg cgtgccgatc cttccggtat tcgccggacc agaaataccg
     5041 ggccgtcatg aagatcagat aggccgctcc cagggctttg aattcccaca tacggatgag
     5101 ccagtatgcg ctgacaaggg agagcgcgcg gaacacaaac gccccgatga taccgtaaag
     5161 gagtgccttt ttctgttcgt tcctgtccgg aagccgggac accagtacgg cgagcacgag
     5221 cgcgttgtct gcggcaagaa gcccctccat aagcaccagg gtgccgatga tggccgagta
     5281 ttcgaggatg tgcgctacca tatcgtgtcc ggacgggaac tgtgcggggc cgcaggtgcg
     5341 cggaaagagt tgagacgtac cacagaaagc tcacgaggac ggggtttacg gaaccagcat
     5401 cgattccttt gcgatcagtt tgtgcaggt
//
LOCUS       DAOSOO010000211         5303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15636_length_5303_cov_5.026105, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000211 DAOSOO010000000
VERSION     DAOSOO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15636_length_5303_cov_5.026105"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLT86_12270"
     CDS             complement(<1..742)
                     /locus_tag="PLT86_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37428.1"
                     /translation="MFERGTTFGREVIEMTDPVTGAFIMQLTSAQNASAKLYFELQSF
                     LCDDSTIIFHSQRVPARGAPMELFRVDTDGRNLTQLTDEAHPLGHPVPAWTTRSVYGL
                     RANVLIRLDVDTLEETEIACCDEAVEGASSSMSGDDRFFFTVATMKDGQKTIVRFATD
                     GSEVAVMLRGIPVNHINADPAGTRFTFNRSLDPDDPGRIQICDFDGGNLRAFPFQRFA
                     HRSWLGRTGRVQGPLAAPARGIGSIAPDE"
     gene            complement(766..1848)
                     /locus_tag="PLT86_12275"
     CDS             complement(766..1848)
                     /locus_tag="PLT86_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37429.1"
                     /translation="MSQRGRTFGRETVEMTDPVTGAFVLQLTAAQNFSTNLYFEHQSF
                     LCDDKTLIFLSQRAPGRGMPYDLFRVDIDGRNMTQLTDEEHPLGHPIPSWDKRIIYGF
                     RKNVLLSLDPDTLEETELNRCDEATGFGACTLSGDDKYFIAVATMKDGTSAIVRLATD
                     GSDVAVMCEGLPYNHIVANPGKPEFTFSGKGSAEFPQGMMVCNNDGTNLRHFAFQQFA
                     HCAWLGRTGRMQGTLLPPGHAIMAIASDETEPTPIASGVYFWHSAASLDAKWIVADTN
                     WPDIGIQLIHVESGRYACLCKSLGSNGDWTHPHPSFNRGGTQVVFNSDRTGVPQIYLA
                     TIPDAIRMQLETGALTHRQRWVGKVI"
     gene            complement(1860..2930)
                     /locus_tag="PLT86_12280"
     CDS             complement(1860..2930)
                     /locus_tag="PLT86_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37430.1"
                     /translation="MAEMTTYERMKRMYEHREADRVPVTDGPWGSTLERWHREGLPTG
                     IPWDDYFGLDKFAGIGADNSPRFPSRVVEETDEYTIAVSPWGATSKNWKHAGGVPEFL
                     DFKVKDWDTWREAKARMQPTRDRVNWQHLEQNYRKWREEGRWITAGFWFGFDVTHSWF
                     IGTAKVLMAMVEDPEWIADILNHELELDLALFQMVWDEGYRFDAIGWPDDMGYKQHTF
                     FSLPMYRQLIKPVHQRAAEWAHAKGLKVHLHSCGDVHTFIPDLIELGIDMLNPLEVKA
                     GMDPVALKKQYGDRLAFHGGINAVNFTDPDALFAEMKRVIPVMKENGGYVISSDHSVP
                     DAVSFEDFKTFVRLAKELGSYT"
     gene            3158..4576
                     /gene="pyk"
                     /locus_tag="PLT86_12285"
     CDS             3158..4576
                     /gene="pyk"
                     /locus_tag="PLT86_12285"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT37431.1"
                     /translation="MIRRTKIVCTIGPQTASIEQLRALVDAGMNVARVNFSHGTHEGH
                     RSVIRAVRALRGQYGEPVGVLADLSGPKIRLGKLPGGSCRVETGQTIILTCDTGGNCG
                     DRFPINYPYLSEDVKVGDSVVMNDGDVSARVTEVRGGDVVCVVEDGGMLGDNKGVNFP
                     DSALRVDAITEKDRRDVRFALEEKVDFLALSFVRRPEDIVSLRQLVAGSPHTPRIIAK
                     IEKREALENFDDILAEADGIMVARGDLGLETELARVPMVQKDLIARARAAGKPVITAT
                     QMLESMIVNPVPTRAEVADVANAILDGTDAVMLSGETAVGRFPVDTVRMMARIAAATE
                     AAHQPANGFRRNADRGVLNPSEAIGQSTAQMAWDLHVVAIVVCTDSGKTGRVVSRFRP
                     AIPIVGATPNEEVARQLTLSWGVVPALLEPITSTDELIAHAFATVKGTGIAKDGDQVV
                     ITAGIPFGGGGMTNLLLVQQIA"
     gene            4694..>5303
                     /locus_tag="PLT86_12290"
     CDS             4694..>5303
                     /locus_tag="PLT86_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37432.1"
                     /translation="MRRSISLILLLALVSIVGCGLFSKKQAANYVTPEGWVGATAKKG
                     SFTLDAVLLDDSSYGLEGAVRFSWWKVFNVTVENRSRQPVSLPLSSFSLVDASGAEQK
                     PLPAEEVQAYQSYVMGPLLSYQRSAVRQAIWSEKEIAPGAFAVGYLFFPRKRHATPYS
                     LVLDPNPNKRKDELVLAFPAQYVEPELPAVAAARDTVTAESAE"
BASE COUNT          983 a         1474 c         1763 g         1083 t
ORIGIN      
        1 tctcgtcggg ggcgatgctg ccgatgcccc gcgccggggc ggccagcggg ccctgcacgc
       61 gcccggtgcg gccaagccat gaacggtgtg cgaaccgctg gaaggggaat gcccgcaagt
      121 tgccgccgtc gaaatcacag atctggatac ggccggggtc gtcggggtcg agactccggt
      181 tgaacgtgaa ccgggttccg gccgggtccg cgttgatgtg attgacggga atgccgcgaa
      241 gcatgacggc gacttcggag ccatcggtgg cgaaccggac gattgttttt tgcccgtctt
      301 tcattgtggc gacggtgaag aagaatctgt cgtcgccgga catgctcgag gaggcgcctt
      361 ccacggcttc gtcgcaacag gcgatttcgg tctcctcaag cgtatccaca tcgaggcgga
      421 taagcacgtt cgcccgcagg ccgtagaccg aacgggtggt ccacgcggga accggatggc
      481 ccagcgggtg ggcctcgtcg gtgagctggg tcaggttgcg gccgtcggtg tccacgcgga
      541 acagttccat aggggcacca cgcgccggca cacgctgcga gtggaagatg atggtgctgt
      601 cgtcgcacag gaacgactgc agctcgaaat agagcttggc gctggcgttc tgcgcggagg
      661 tgagctgcat gatgaatgcg ccggtgaccg ggtcggtcat ctcgatcact tcgcggccga
      721 aggtggtgcc ccgttcaaac atttcgtgct ccgagagaac cggaatcaga tcactttccc
      781 tacccagcgc tgacgatgcg tgagagcgcc ggtttcgagt tgcattcgga tcgcgtcggg
      841 aatcgtcgcg agataaatct gcgggacgcc ggtgcggtcg gagttgaaga caacctgcgt
      901 gccgccgcgg ttgaacgacg gatgcgggtg cgtccagtcg ccgttgctgc cgagggattt
      961 gcacaggcag gcgtagcggc ccgattcgac gtggatgagc tggatgccga tatccggcca
     1021 gttggtgtcg gcgacgatcc acttcgcgtc gaggctggcg gctgagtgcc agaagtagac
     1081 gccggatgcg atgggtgtgg gctctgtttc gtcggaggcg atggccataa tggcgtggcc
     1141 gggcggcagg agcgttccct gcatacggcc ggtgcgcccc agccaggcgc agtgggcgaa
     1201 ctgctggaag gcgaagtgcc ggaggttggt cccgtcgttg ttgcagacca tcatgccctg
     1261 cgggaactcg gcactgccct tgccgctgaa ggtgaattcc ggttttccgg ggttggcgac
     1321 gatgtggttg tagggcagac cctcgcacat gacggccacg tccgagccgt ccgtcgcaag
     1381 ccgcacgatt gcgctcgtgc cgtctttcat ggttgcaacg gcgatgaagt atttgtcgtc
     1441 gccggagagc gtgcatgcgc cgaagccggt tgcctcgtcg cagcggttca gttcagtctc
     1501 ctcaagcgta tcggggtcaa ggctcagaag cacatttttg cggaagccgt agatgatccg
     1561 cttatcccac gaggggatcg gatggcccag cgggtgttcc tcgtccgtca actgggtcat
     1621 gttccggccg tcaatgtcca cgcggaacag gtcgtacggc atgccgcggc cgggagcgcg
     1681 ctgagagagg aagatgagcg tcttgtcgtc acacaggaac gactgatgct cgaagtagag
     1741 gttggtgctg aagttctgtg cagcggtaag ctgcaggaca aatgcgccgg tgaccggatc
     1801 ggtcatttct accgtttcgc ggccgaaggt tctgcctcgt tgcgacatgg tggttttcct
     1861 tacgtgtagg acccgagttc ttttgccaat ctgacgaagg tcttgaagtc ctcgaagctt
     1921 acggcgtcgg gaaccgagtg gtcagagctg atcacgtacc caccattttc cttcatcacc
     1981 ggaatcacgc gtttcatttc cgcgaacagg gcatccggat ccgtgaagtt gacggcgttg
     2041 atacctccgt ggaacgcgag tctgtctccg tactgctttt tgagcgcgac cgggtccatg
     2101 cctgccttca cttccagcgg gttgagcatg tcgatgccaa gttctatgag gtcggggatg
     2161 aaggtgtgaa cgtcgccgca ggagtggagg tgcactttga ggcctttcgc gtgcgcccat
     2221 tccgctgccc gctggtgcac cggtttgata agctgccggt acatcggcaa cgaaaagaac
     2281 gtgtgctgct tgtagcccat gtcatccggc cagccgatgg cgtcaaaccg gtagccttcg
     2341 tcccacacca tctggaacag cgccaggtcc agttccagct catggttgag gatgtcggcg
     2401 atccattcgg ggtcctccac catggccatg agtactttcg cggtgccgat gaaccatgaa
     2461 tgcgtcacgt caaaaccgaa ccagaagcct gcggtgatcc agcggccttc ctcgcgccac
     2521 ttcctgtagt tctgttcgag gtgttgccag ttcacgcggt cgcgcgtggg ctgcatgcgg
     2581 gctttcgctt cgcgccaggt gtcccagtct ttcactttaa agtcaaggaa ttccgggacg
     2641 ccgcccgcgt gtttccagtt tttcgacgta gcgccccacg ggctgacggc aatcgtgtat
     2701 tcgtcggttt cctcgaccac gcgggacggg aaacggggac tgttatccgc gccaatgccg
     2761 gcgaatttgt cgaggccgaa gtagtcgtcc cacgggatac cggtgggcag gccttcgcga
     2821 tgccagcgtt ccagcgtgga accccatggc ccatcggtaa ccggaacgcg gtcggcttcc
     2881 cggtgctcgt acatgcgctt catgcgctcg taggtagtca tttctgccat gttcgcagtt
     2941 ctccctgagg cgggatctgg ttgctcggtg tgtggccgaa tgccgggaaa aaagatacct
     3001 gccgctccgg caagaccaaa ccaaccttgt aagacgggac ggtgcgtctt actttgtgaa
     3061 ccgatggcgt accctgcccc gctcacgaaa aacacatcca agagccttaa tctgaagaga
     3121 cgacacgtta gctggatttt tccgtgggaa ggaaacaatg atccgtcgaa ccaagattgt
     3181 ctgcacgatc ggcccgcaaa cggcctcgat tgaacaactt cgcgcgctgg ttgatgcggg
     3241 gatgaatgta gcgcgcgtga atttctcgca cggcacgcac gaggggcacc ggtctgtcat
     3301 aagggcagtt cgtgcgttgc gagggcagta cggcgaaccg gtgggagtgc tggcggatct
     3361 ttcggggcca aaaatccgcc ttgggaagct gcccggcggc tcgtgcagag tggaaaccgg
     3421 ccagacgatc atccttacct gcgatacagg gggaaactgc ggcgaccggt tcccgatcaa
     3481 ctacccctat ctttccgagg acgtgaaggt cggcgactcg gtggtaatga acgacggcga
     3541 cgtgagcgcg cgagtgacgg aggtccgcgg cggggacgtg gtgtgtgtgg tggaagacgg
     3601 gggtatgctg ggagataaca agggggtcaa tttcccggac tccgcgctgc gcgtggacgc
     3661 gatcaccgag aaggatcggc gcgacgtgcg ttttgcgctg gaagagaagg ttgatttcct
     3721 cgcgctttcg ttcgtacgga gaccggagga cattgtctca ttgcgccaac tggttgcagg
     3781 aagcccgcac acgccgagga ttatcgccaa gatcgagaag cgggaggcgc tggagaactt
     3841 cgatgacata cttgccgagg ctgacggcat catggtggcc cggggagacc tgggtctgga
     3901 gaccgaactg gcgcgcgtgc cgatggtgca gaaggacctg atcgcccgtg cgcgggcagc
     3961 gggaaagcca gtcatcacgg cgacgcagat gctggaatcc atgattgtga acccggtgcc
     4021 aacacgggcc gaggtcgccg atgtggcgaa cgcaattctg gacggcaccg acgcggtgat
     4081 gctgagcggg gagactgcgg tgggacgttt cccggttgac acggtgcgca tgatggcaag
     4141 gattgccgcc gcgacggaag cagcacacca gcctgccaat ggcttccggc ggaatgcgga
     4201 ccgaggtgtg ttgaacccat ccgaggcaat cggccagagc accgcgcaga tggcgtggga
     4261 cctgcacgtg gtggcgatcg tcgtgtgcac ggattcggga aagaccggcc gggtggtcag
     4321 ccgtttccgg cccgcgattc ccatcgtagg cgcaacgccg aatgaggaag tcgcccgcca
     4381 gttgacgctt tcatggggag ttgttccggc gcttctggaa ccgatcacct ctactgacga
     4441 gctgattgcg catgccttcg ccacggtaaa gggcacaggc attgccaaag acggtgatca
     4501 ggtagtgatt actgcgggca tcccgtttgg cgggggcggt atgacgaact tgctgcttgt
     4561 ccagcaaatc gcctgattgc gggcgcgcgt ggaaaaactt ggcgtgcact tgcacttgcg
     4621 acatgtattt tcgtactctc acactcgtga aaagccggca ttgtgttgga cgtttttcca
     4681 aggggacacg agagtgcgac gcagcatttc cctgattttg ttactcgcgc tcgtttccat
     4741 tgtggggtgc gggcttttct cgaaaaagca ggccgcgaat tacgtaacgc cagagggctg
     4801 ggtcggcgcg acggcgaaaa agggttcgtt cacgcttgac gcggttctgc tggacgattc
     4861 cagctacggt cttgagggcg ccgtgcggtt ttcctggtgg aaggtgttca acgtaaccgt
     4921 ggaaaaccgt tcgcgccagc ctgtcagcct tcccctgtcc tcgttttccc tggttgatgc
     4981 ctctggcgcc gaacagaaac cgcttcccgc cgaggaggtg caggcatacc agagttacgt
     5041 gatggggccg cttttgagct accagcgcag cgccgtccga caggcaatct ggtcggaaaa
     5101 ggagatcgcg ccgggggcgt tcgcggttgg ctacctcttt ttcccgcgca agcggcatgc
     5161 gacgccgtac agccttgttc tggacccaaa cccgaacaag cgcaaagatg aactggttct
     5221 ggcgtttccg gcacaatacg tggagccgga gcttcctgca gtggccgcgg ctcgtgatac
     5281 agtgactgcc gaatcggcgg aac
//
LOCUS       DAOSOO010000212         5267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15775_length_5267_cov_6.362817, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000212 DAOSOO010000000
VERSION     DAOSOO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15775_length_5267_cov_6.362817"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLT86_12295"
     CDS             complement(<1..183)
                     /locus_tag="PLT86_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT37433.1"
                     /translation="MDILKGDLVEVISGDSRGPRRGEGKRGKVLFVDRAKGKVIVEGV
                     NIIRRHTKPSREHPQGG"
     gene            complement(208..576)
                     /gene="rplN"
                     /locus_tag="PLT86_12300"
     CDS             complement(208..576)
                     /gene="rplN"
                     /locus_tag="PLT86_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT37434.1"
                     /translation="MIQQQTNLVVADNTGAKRAMCIKVLGGTRARYATVGDQIIVAVK
                     EAIPGGAVKKGDVARGVVVRVSRATRRPDGSHVRFDDNAVVLINDQGEPRGTRIFGPV
                     ARELREHKFMRIVSLAPEVV"
     gene            complement(592..855)
                     /gene="rpsQ"
                     /locus_tag="PLT86_12305"
     CDS             complement(592..855)
                     /gene="rpsQ"
                     /locus_tag="PLT86_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT37435.1"
                     /translation="MTSETRAHRKTRVGRVVSAKPDKTIVVVIDRLVRHSLYERIVRR
                     TKKLYVHDEQNEAHVGDTVEAMETRPLSKTKHWRLVRIVERAR"
     gene            complement(852..1088)
                     /gene="rpmC"
                     /locus_tag="PLT86_12310"
     CDS             complement(852..1088)
                     /gene="rpmC"
                     /locus_tag="PLT86_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT37436.1"
                     /translation="MKTRDIRKMSIEEVRVHLREAEEEVFRLRFRAATEQLESPIMLR
                     HRRRDIARMVTIIREHEQGVRTLAQRESVEGATS"
     gene            complement(1094..1516)
                     /gene="rplP"
                     /locus_tag="PLT86_12315"
     CDS             complement(1094..1516)
                     /gene="rplP"
                     /locus_tag="PLT86_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT37437.1"
                     /translation="MLMPKRVKHRKQQRGRRKGLALRGSAIHFGQYGLQAVEPAWVTD
                     RQLEAARVAMTRYIKRGGKVWLRVFPDKPVTAKPAETRMGKGKGVPDHWVAVIKPGRI
                     LFEMEGIDEATAQEAMRLANSKLPVKTRFIQRSETVQG"
     gene            complement(1523..2239)
                     /gene="rpsC"
                     /locus_tag="PLT86_12320"
     CDS             complement(1523..2239)
                     /gene="rpsC"
                     /locus_tag="PLT86_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT37438.1"
                     /translation="MGQKTHPTGLRLGVLRTPERPNGLWKSRWYGGRDFPALLKEDQM
                     LRAYVLQRLQKASVSLIEIERQPKRISITVHTARPGIVIGRKGQEVDKLRDELRHLTK
                     KDVFINIEEIKKPELDAELVAQNIRRQLEGRVLFRRAMKKALENTMRMGAQGIRVVCS
                     GRLGGAEMSRTERYIRGRVPLHTLRADIDYALVQALTTMGMIGVKVWICRGEQIGDDF
                     PTAPRRAEGTASGMSEGARR"
     gene            complement(2255..2617)
                     /gene="rplV"
                     /locus_tag="PLT86_12325"
     CDS             complement(2255..2617)
                     /gene="rplV"
                     /locus_tag="PLT86_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT37439.1"
                     /translation="MALIARAVSKNQWASARKVRLVADLVRGKTVGTALNVLHFSPSS
                     ASLQVEKTVRSAMANLLEKEQGQRLVEDDVVISQITVDGGMIYKRIRPRAMGRAFRTR
                     RRTCHITVEVAARERTER"
     gene            complement(2646..2951)
                     /gene="rpsS"
                     /locus_tag="PLT86_12330"
     CDS             complement(2646..2951)
                     /gene="rpsS"
                     /locus_tag="PLT86_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT37440.1"
                     /translation="MARSIKKGPYIEPSLQKKVDELNRTGEKRVIKTWSRRSTISPDF
                     VGHTLGVHNGNKFIPIYVTENMVGHKLGEFAPTRIFRGHPGSGGGGEKTAKVAPSAK"
     gene            complement(2963..3799)
                     /gene="rplB"
                     /locus_tag="PLT86_12335"
     CDS             complement(2963..3799)
                     /gene="rplB"
                     /locus_tag="PLT86_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT37441.1"
                     /translation="MPFKKFRPLTPSLRYTTTDTFEDVTTGTPERSLVEKLHRTSGRN
                     NSGRITSRRRGGGHRRLYRVIDFKRDKFGIPAKVKSIEYDPNRTARIALLVYADGEKR
                     YIIAPDGIAVGDELMSGPTAPVRPGNALPLTHIPAGSTVHNVEMKPGKGAALARSAGA
                     LVQLISREGDMARLRMPSGEVRLVPSVCMATLGQVGHVDRDDRSLGKAGRNRWLGRRP
                     SVRGVAMNPVDHPHGGGEGRTSGGGGKGSHPRTPWGVITKGKKTRNPKKSNRLIISRR
                     TK"
     gene            complement(3802..4104)
                     /gene="rplW"
                     /locus_tag="PLT86_12340"
     CDS             complement(3802..4104)
                     /gene="rplW"
                     /locus_tag="PLT86_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT37442.1"
                     /translation="MSSPYTIVKRPVVSEKVNLQQERFNQYTFEVDRASNKHEIQRAI
                     EELFSVKVTSIRVIHVRGKKRRMGMRYRKLGRTASWKKAIVTLEKGQTIDILGERV"
     gene            complement(4101..4775)
                     /gene="rplD"
                     /locus_tag="PLT86_12345"
     CDS             complement(4101..4775)
                     /gene="rplD"
                     /locus_tag="PLT86_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT37443.1"
                     /translation="MNAVRFDEAGAEAGRVDLPATLFGAEPNLDVVHKAVVTYLANQR
                     QGTAKTKTRSEVAGTNKKPFKQKKTGQARRGDMKTNVQRGGGVYGGPIPRDFRQKLPK
                     KMRQLALRSALSIRAKEDAVSAIADISLESGKTRDVAALLKNVGLQDKRVLFVADKSP
                     ATLQRASRNIENFWLTSVGSLSTYAVMWAERVVFTESALARLNEAGLNEGSLAGAGVS
                     EAGGAQ"
     gene            complement(4780..>5267)
                     /gene="rplC"
                     /locus_tag="PLT86_12350"
     CDS             complement(4780..>5267)
                     /gene="rplC"
                     /locus_tag="PLT86_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT37444.1"
                     /translation="AVIVGVEEDSKRRAPRQIAGQYPENVSPRKLIREIKLESAPEIG
                     STVDASVFSVGDRVKVVGTSKGRGFAGFVKRHHFKGGPNTHGSMSHRLPGSIGSSAYP
                     SRVIKGVKGAGHLGNQSSTVKGLRVVEVDAERNLLVLSGCVPGWRNGLITISSDDRGK
                     A"
BASE COUNT          960 a         1748 c         1386 g         1173 t
ORIGIN      
        1 gccaccctgc ggatgctcac gcgagggttt tgtgtgccga cgaatgatgt tcacaccctc
       61 gacaatcact tttcccttgg ctcgatcaac gaaaagcact ttgccccgct tgccctcgcc
      121 tcgcctgggc ccacggctgt ccccgctgat cacttcgacg agatcgccct tcaatatgtc
      181 cacgacttca atcccctcgg ctcgtgctca gacaacttcc ggagccagcg aaacaatgcg
      241 catgaacttg tgctcacgaa gttcgcgagc aaccggacca aaaatacgcg tacccctcgg
      301 ttcgccctga tcattgatca acaccaccgc gttgtcatca aagcgaacat gggagccatc
      361 cggccgccgc gttgcgcggg acactctcac caccacacca cgcgcgacgt cgcccttctt
      421 gactgccccg ccggggatcg cctctttcac tgcgactatg atctgatccc cgactgtggc
      481 gtaccgcgca cgtgttccac cgagcacctt gatgcacatg gcacgcttcg cgccggtatt
      541 gtcggccacc acgagatttg tctgttgctg aatcatggcg aaaaagcctt cctaccgggc
      601 acgttccaca atacggacca gccgccagtg tttggttttg ctcagcggcc tggtctccat
      661 agcttccacg gtgtcaccca cgtgcgcttc attttgttcg tcgtgtacgt aaagcttctt
      721 cgtccggcgg acgatgcgct catacagcga gtgacgcacg aggcggtcta tcaccacaac
      781 tatcgtcttg tcaggctttg cacttaccac acggccaacg cgtgttttgc gatgggcacg
      841 agtttcactt gtcatgatgt cgccccttca accgattccc gttgggcaag cgtacggacg
      901 ccctgctcgt gttcgcggat gatcgtgacc atccgggcga tatcccggcg ccggtgtcgg
      961 agcatgatag ggctctccaa ctgttcggtg gcggcgcgga aacggaggcg gaacacctct
     1021 tcctctgcct cacgcaggtg cacgcgaacc tcttcgatgg acatcttgcg aatgtcacgt
     1081 gttttcatca ccgtcacccc tgaaccgttt cagaacgctg aatgaaccgc gtcttgacgg
     1141 gaagtttcga attggcgagc cgcatcgctt cctgtgcggt agcctcgtca ataccttcca
     1201 tctcgaacag gatccgaccg ggtttgatca ccgcaaccca gtggtcaggc acacccttcc
     1261 ctttgcccat gcgggtttcc gcgggcttcg cggtaaccgg cttatccggg aacaccctca
     1321 accagacctt ccctccgcgc ttgatgtagc gcgtcatggc aacacgagcg gcttccagtt
     1381 gacgatccgt cacccacgcc ggttcgaccg cctgaaggcc gtattgcccg aaatgaatcg
     1441 cggagccccg aagagcaagg cctttccgcc gcccccgctg ctgtttccgg tgtttgacac
     1501 gtttcggcat taacatcgct gtttatctcc tcgcgccttc ggacatgccg cttgccgtgc
     1561 cttccgcacg ccggggtgca gtcgggaagt catcgccgat ctgctcgcca cgacaaatcc
     1621 acactttcac gccgatcata cccatggtcg tcaacgcctg aaccagcgcg taatcaatat
     1681 ccgcccgcag cgtgtggagc ggcacgcgcc cacggatgta ccgttcggtg cgagacattt
     1741 ccgcaccacc aagccgcccg gaacagacca cgcgaattcc ctgggctccc atgcgcatcg
     1801 tgttttccag cgccttcttc atggcgcgcc ggaaaagcac gcggccttca agctgccgac
     1861 gaatgttctg agcaaccagt tccgcatcca gttcgggttt cttgatctct tctatgttga
     1921 tgaacacgtc tttcttcgta aggtgacgga gctcgtcacg cagtttgtcc acttcctgcc
     1981 ccttacgccc gatcacgatc cctgggcggg cggtgtggac ggtgatgctg atccgcttcg
     2041 gctgccgctc aatctcgata agcgataccg atgctttctg aaggcgctgc agaacgtatg
     2101 cgcggagcat ctggtcttcc ttcagaagag ccgggaagtc gcgcccaccg taccagcgcg
     2161 atttccaaag tccattcgga cgttcgggag tccggagcac tccgagtcga agtcccgttg
     2221 gatgagtttt ctgtcccact aagacgctcc cttcctagcg ttctgtgcgt tcgcgtgcag
     2281 cgacttcaac cgtaatgtgg caggtccggc ggcgcgtgcg gaacgcacga cccatcgccc
     2341 gcggacgaat ccgcttgtaa atcatcccgc cgtctactgt tatctgcgaa ataaccacgt
     2401 catcttccac aagacgctgg ccctgttcct tttccagaag gttcgccatc gccgagcgca
     2461 ccgtcttttc gacctgcagg ctggcggaag acggggaaaa gtggagcacg ttcaatgcgg
     2521 ttccgactgt ctttcccctc accaaatccg cgacaaggcg caccttacgg gcagatgccc
     2581 actggttctt gctgacagcg cgagctatga gtgccatcgt tcccgattcc ttctatacac
     2641 tggtatcact tggccgaggg cgctactttc gctgtctttt cgccaccgcc accggaaccc
     2701 gggtgccccc ggaaaatccg tgtcggcgcg aactcaccca gcttgtgccc aaccatgttt
     2761 tccgtcacgt aaatggggat gaacttgttg ccgttgtgca ccccgagcgt gtgccccacg
     2821 aaatccggcg aaatcgtcga acgccgcgac cacgtcttga taacccgttt ctcacccgtg
     2881 cggttgagct cgtcaacctt cttctgcaga ctgggctcga tgtagggccc cttcttaatg
     2941 gaacgtgcca tgtaccattc ctctacttcg tgcgccgact gatgatgagg cgattggatt
     3001 tcttaggatt ccgcgtcttc ttgcccttgg tgattacgcc ccacggagtt ctcggatggg
     3061 agcctttgcc accaccgccc gaagtgcgcc cttcaccacc gccatgggga tgatccaccg
     3121 ggttcatcgc aacaccgcgg acgctcgggc gtcggcccag ccagcgattc cggccggctt
     3181 tcccaagtga gcggtcatcg cgatccacgt ggcccacctg gcccagcgtt gccatgcaca
     3241 ctgagggaac cagccgcact tccccggaag gcatgcgaag ccgcgccatg tcaccttcac
     3301 gtgaaatgag ctgcacgaga gcaccggcgc tgcgcgcaag agctgctccc ttgcccggct
     3361 tcatctccac attgtgcacg gtggaacccg caggaatgtg cgtcaaagga agcgcatttc
     3421 ccggccgcac gggcgccgtc ggcccggaca tcaattcgtc gccgaccgcg atgccgtccg
     3481 gagcaatgat gtaccgcttc tcgccgtccg cgtacaccag cagggcaatc cgcgcggtcc
     3541 ggttcggatc atattctatc gacttcacct tcgcggggat gccgaacttg tcccgtttga
     3601 aatcaatcac gcgatacaaa cggcgatgcc cgcccccacg tcgacgggag gttattcttc
     3661 cggagttatt gcggcccgac gtgcggtgga gtttctccac cagcgaacgc tccggcgtgc
     3721 ccgtcgttac gtcttcgaac gtatccgtcg tcgtatatcg caagcttggg gtgagcgggc
     3781 gaaacttctt gaacggcatc gctataccct ttcgccgaga atatcaatcg tctgcccttt
     3841 ttcgagcgtg acgatcgctt ttttccagga cgccgttctt ccgagtttcc ggtaccgcat
     3901 gcccatgcgc cgctttttgc cgcgcacgtg gatcacccgg atcgacgtaa ccttcacact
     3961 gaaaagctct tcgatggcgc gctggatctc gtgtttgttc gaagcgcgat ccacttcgaa
     4021 ggtgtattga ttgaaacgct cctgctggag attcactttc tccgacacca cggggcgctt
     4081 gactatcgta tagggggaac tcattgagca cctcctgcct cactgacgcc tgccccggcg
     4141 aggcttccct cgttgaggcc tgcctcattc agacgggcaa gggctgactc ggtaaacaca
     4201 acccgctccg cccacatcac cgcgtacgtg ctcaatgaac cgacagacgt caaccagaag
     4261 ttctctatat tccggcttgc ccgctgcagt gtcgccgggg acttgtcggc gacgaacaac
     4321 acgcgcttgt cctgcaatcc gacattcttg agcaacgccg ccacgtcccg cgtctttccg
     4381 gattccagcg agatatccgc tatcgcggaa accgcgtctt ccttcgcccg gatcgaaagg
     4441 gcggaacgca gagccagttg ccgcatcttc ttgggcaact tctggcggaa atcccgcgga
     4501 attggtccgc cgtagacgcc gcccccgcgc tgcacgtttg ttttcatgtc gccgcggcgc
     4561 gcctgcccgg tctttttctg tttgaagggc ttcttgttcg tgccggcaac ttccgaacgg
     4621 gtcttggttt tggcggtacc ctgacgctga ttcgcgaggt acgtcaccac tgccttatgc
     4681 accacgtcca gattgggctc ggccccgaag agcgtcgcgg gcaggtctac ccgccccgct
     4741 tcggctcccg cttcatcaaa ccgcactgcg ttcatagttt caagccttac cacgatcgtc
     4801 cgaagaaatg gttatcagac cgttgcgcca cccggggacg cacccgctca gcaccagaag
     4861 gttacgttct gcatcaactt ccaccacgcg caaacccttg acagtgctgc tctggttgcc
     4921 caggtgtccg gcacccttca cgcccttgat gacgcggctg ggatacgccg acgagccgat
     4981 cgaccccggc aagcggtggc tcatgctccc gtgcgtattg ggtccgccct tgaagtggtg
     5041 gcgtttcacg aaaccggcaa aaccgcgccc tttgcttgtg ccaaccactt tcaccctgtc
     5101 acccacggaa aacacgctgg cgtctaccgt ggagccaatc tcaggcgctg attcgagctt
     5161 gatttcccga atgagcttcc tcgggctgac gttttccggg tattgccctg caatctgacg
     5221 gggcgcacgt cgctttgaat cttcttccac gccgacaata acggcat
//
LOCUS       DAOSOO010000213         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_15796_length_5263_cov_6.070084, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000213 DAOSOO010000000
VERSION     DAOSOO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_5263_cov_6.070084"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLT86_12355"
     CDS             complement(<1..323)
                     /locus_tag="PLT86_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT37445.1"
                     /translation="MGNKPVWGRFEELRPDQLEQIVEQWPVAYWPLGLLEHHGWALPV
                     GFDGVKAVRFCERIAAATGGVVLPCLWWGSHGGHGDFKWTFYQDPVVGERVLDVTVRK
                     LIDFG"
     gene            complement(545..2236)
                     /locus_tag="PLT86_12360"
     CDS             complement(545..2236)
                     /locus_tag="PLT86_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT37446.1"
                     /translation="MRVEAFFAACALLAMSSCASLEVPDTPPHETAVRQQSPRPEAIL
                     RLLAAKRAERRGDSAGALAQWQAAVRADSSSPSLRLGLAKAYSVQRNDSLALLNGRRS
                     VLLDSNYVDGHLFLAGLYEGRNDFASAATHLEAAFRESKSPETGWQLARLYARLAKPE
                     ESRRVYQTMADSPTAHPDDVLAWAEQVQMSPVKGASDIFYTTCMRRWPGNEEGVGAYA
                     EYLARSGRRVEAERLLREWSASHPDSREVPKRYIQLLVAQDRWADADSVWMRIRRESP
                     DDLVEQKGWITYLTKRGQIPLAIANAQRLIETHPSDGDGYVLLGQAHAAQGDLHAAAA
                     AFEQAASQDSSLEALSELAYAQAGLKQYAAAERAARQGLSKYPGDERLLSLYSAALSS
                     QNKWREAVPVLRALAERDTTDTDRLFDLGAGLERAGEFDSAVVVFKRLLAINRHHADA
                     LNYLGFMFADRNVHLAEAESLLTEAVRLEPRNAAFLDSMGWLFFRQGRYQEAKQHLAD
                     AIEIDDSVAEMHVHLGDVLSALGDRDNAREAYRAALRLDPANRTVERKLRLLERR"
     gene            complement(2376..2639)
                     /gene="rpmA"
                     /locus_tag="PLT86_12365"
     CDS             complement(2376..2639)
                     /gene="rpmA"
                     /locus_tag="PLT86_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018179605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT37447.1"
                     /translation="MAHKKGVGSSRNGRDSNAQRLGLKRFGGELVNAGTIIVRQRGTR
                     FHPGLHVGRGGDDTLFALATGHVKFAPHKNGKGKVVSIEPLET"
     gene            complement(2664..2978)
                     /gene="rplU"
                     /locus_tag="PLT86_12370"
     CDS             complement(2664..2978)
                     /gene="rplU"
                     /locus_tag="PLT86_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT37448.1"
                     /translation="MQAVVRVAGVQARVAPGDKIFLPRIPGEIGTVTTLGEVLLLSDG
                     DKITVGKPVVEGASVQAEVLDHARDAKVLVFKKKRRKRYRRLRGHRQSYTQVLIKEIV
                     AA"
     gene            complement(3070..4623)
                     /locus_tag="PLT86_12375"
     CDS             complement(3070..4623)
                     /locus_tag="PLT86_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT37449.1"
                     /translation="MKTEIIVNSATQETRIAILEDGRLVEILVERPDTQRLVGNIYKG
                     IVTSILPGMQAAFVDIGMEKSAFLHARDVGGSPERLDDEEDEDEEDDDEETPRSGRET
                     KKYTPIQELLTKGQEVIVQITKEPIGTKGPRVTTDVSLPGRFSVLMPYSNHVGVSRKI
                     LSWSEKRRLKALAARLRPENCGIIVRTVAEGQGEDDLTRDIDDLKRTWERVHRRAKRM
                     KAPALIHSELGMTTSLMRDLLSEDVERVVVDSKSVYNDIIKYLKGTAPQLRDRVLLYD
                     QKEPVFDAYGIEQEIANATNRRVPLRKGGFLVIDHTEAMVTIDVNTGRYVGRNNQEQT
                     IVNINLEAGQEIARQLRLRDIGGIIAIDFIDMAIEANRKKVWEEFNKALRHDRSRINV
                     AERLSEFGVLEMTRQRVRPSLLYTFSEPCPLCKGSGRVQSPDTTVTKLERWLKRLRAA
                     GGEKKLVLEVHPTVGSYLRENDQRILKALRRTTGAQIILEDNPSLEIDAYRFSSLKTA
                     EDLTGKYEA"
     gene            complement(5028..>5263)
                     /locus_tag="PLT86_12380"
     CDS             complement(5028..>5263)
                     /locus_tag="PLT86_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37450.1"
                     /translation="PLFWPFLSLLRHRCAFWGLVEIRAIGRQTRVQCATKNKFGAVAV
                     RIYLQLGFAARHCSADRKLNGKLERTATDERST"
BASE COUNT         1011 a         1628 c         1464 g         1160 t
ORIGIN      
        1 aaaccgaagt cgatcagctt tcggacggtt acgtcgagaa ctcgttcgcc aaccacaggg
       61 tcctggtaga aggtccattt gaagtcgccg tgtccgccgt ggctgcccca ccacaggcat
      121 ggcagaacta cgccgcctgt tgctgccgcg atgcgctcgc agaaacgtac agccttgacg
      181 ccgtcgaagc ccaccggcaa cgcccacccg tggtgctcaa ggagccccag tggccagtac
      241 gccaccggcc attgttccac gatttgctcc aattgatcgg gacgcagttc ctcaaagcgc
      301 ccccacacgg gcttgtttcc catcctgcca actccctgcc ctgaacgttc cgtttgttct
      361 tctgagtcaa cctcggtccc gcgcgttcgc cttccggtac cggggacctt gcgcgtaccg
      421 gccggaacag gaaccgaccg accgcggctt caacgtaaaa tatggtgagc gccgcgttgc
      481 ggacaaccgg aactgcgggg aatgcgcgtg gttgaggttt gcctgtgctg ccgagggccg
      541 cctgtcaacg ccgctccagc aaacgcaact tgcgttccac ggtgcgattt gccggatcga
      601 gccttagagc ggcgcggtac gcctcacgtg cgttgtcacg gtctccgagc gcggacaaca
      661 catcgccgag gtgcacatgc atttccgcaa ccgagtcatc aatctcgatg gcgtcggcaa
      721 gatgctgttt cgcttcctgg taacgcccct gccggaagaa cagccatccc atgctgtcaa
      781 ggaacgccgc attccttggc tcgaggcgga ccgcttccgt gagaagagat tcagcctccg
      841 cgaggtgaac gtttcggtcg gcaaacatga agccaaggta gttgagcgcg tccgcgtgat
      901 gcctgttgat ggcgaggagc ctcttgaaca ctaccactgc gctgtcaaac tcgccggcgc
      961 gctcgagacc cgcgcccagg tcgaacagcc gatcggtgtc ggtagtgtca cgttctgcaa
     1021 gtgcccgaag gaccggcact gcttcccgcc atttgttctg ggaggaaagt gcggcgctgt
     1081 aaagggaaag gagccgttcg tctccgggat attttgagag cccctgccgt gcagctcgct
     1141 cagcagcagc gtattgcttg aggcctgcct gtgcgtaggc cagttcgctc agcgcctcga
     1201 gagatgaatc ttgacttgcc gcctgttcaa aggctgccgc tgccgcgtga aggtctccct
     1261 gagcagcatg cgcctgcccg agaagaacgt acccgtcacc atcggaaggg tgcgtctcga
     1321 tgagtcgttg cgcgttcgcg attgccaggg gaatctgtcc ccgtttggtg agatacgtaa
     1381 tccagccttt ttgctcgacg agatcatccg gggattctct ccgaatccgc atccagaccg
     1441 aatctgcatc cgcccagcga tcctgggcga cgagcaactg gatgtatcgt ttgggcactt
     1501 cgcgggaatc cgggtggctg gcagaccatt cccgcagcag tcgttctgcc tccacgcggc
     1561 ggccgctccg ggcgagatac tccgcatacg cgcctacacc ctcctcattg cccggccacc
     1621 gacgcatgca ggtcgtgtag aagatatcag aggcgccctt gaccggcgac atctggacct
     1681 gttccgccca tgccagcacg tcgtcaggat gtgcggtggg agaatccgcc atcgtctggt
     1741 acacacggcg gctttcttcg ggcttcgcca gacgcgcgta caaacgggca agctgccatc
     1801 ccgtttccgg agatttcgac tctctgaagg cagcctcaag gtgcgtcgcg gcgctcgcga
     1861 aatcgttgcg gccctcgtac aagccggcaa gaaaaagatg cccatccacg tagtttgagt
     1921 cgagcagaac acttcttcga ccgttcagaa gcgcaagtga atcgttcctc tgcacggagt
     1981 acgccttcgc caaccccagc cggagtgatg gggacgagga atcagcccga actgccgctt
     2041 gccactgcgc aagtgccccg gccgaatctc cccgacgttc tgcgcgtttg gcggccaaga
     2101 gccggagaat agcctcggga cgagggcttt gctggcggac agccgtttcg tgaggcggtg
     2161 tatccggtac ctcaagcgag gcgcaggaac tcattgccag cagggcacat gccgcaaaaa
     2221 aggcttccac acgcatcgaa ctgaattccc ttccattgcg cgtccttttc cgtgtcattc
     2281 actgaaaagg tacgcaaaac ctgtgctgag gggtaaaaaa aagaccgccc acccgcaaag
     2341 ggtggcgcga tctttctgag ctcttggtca aaagttcagg tttcgagtgg ttcgatgctt
     2401 accacttttc ccttaccgtt cttatggggg gcgaacttta cgtgccctgt cgccagcgcg
     2461 aaaagcgtat catcaccgcc cctgcccacg tggagacccg gatggaaccg ggtgccccgc
     2521 tggcgaacga tgattgtccc ggcattcacc agctcgccac cgaagcgctt gagaccgaga
     2581 cgctgcgcat tggaatcgcg cccgtttcga gaacttccga cgcctttctt atgtgccatt
     2641 cgaagaacct ttccaagcag agcctatgca gccacgattt ccttgatgag cacttgcgta
     2701 taggactgac ggtgcccccg aaggcgccga taccgtttcc gccgcttctt cttgaacacg
     2761 agaactttcg catcgcgcgc atgatccagc acttcggctt gcacgctggc accttcgacc
     2821 accggtttgc caacggtgat cttgtcgcca tcggaaagaa gaagaacctc tcccagggtg
     2881 gttacggtgc cgatttctcc gggaatccgc gggaggaaaa tcttgtctcc cggcgccact
     2941 ctggcctgca ctccagcaac tcgcactact gcctgcatga ttttcgccta gtttcggttt
     3001 cgtatgttgg gaaaaaagaa cgggtcaaaa gtactagact ttttaagtta ccacccgtcg
     3061 gagcggctgt caagcctcgt atttacctgt caggtcctcc gcggtcttca aactggagaa
     3121 gcggtacgcg tcgatttcca gcgaagggtt gtcctcaaga atgatctgag cgcccgtggt
     3181 tcgccgcagg gctttgagga tcctctggtc gttttcacgc agataggagc caaccgtggg
     3241 atgaacttcg agaaccagtt tcttctcgcc tccggcggct cgcagccttt tcagccaccg
     3301 ctcgagcttc gtcaccgtcg tgtcaggact ctgcacgcga cccgatccct tacagagcgg
     3361 acacggttca ctgaacgtgt aaagcaggct cggacggacc cgttgccggg tcatctcgag
     3421 aacaccaaac tcgctgagtc gttccgcgac attgattcgc gaacggtcat gccgcagcgc
     3481 tttgttgaac tcttcccaca cctttttccg gtttgcttca atcgccatgt cgataaaatc
     3541 gatggcgatg atgccgccga tatcgcggag gcggagctgt cgcgcgatct cctgccctgc
     3601 ttcgaggttg atgttaacga tcgtctgttc ctggttgttc ctccccacat accgccctgt
     3661 attcacgtcg atcgtaacca tcgcttcggt gtgatctatc acaaggaacc cgccttttcg
     3721 caatggcacg cggcgattcg tcgcgttcgc gatttcctgc tcaatgccgt atgcatcgaa
     3781 cacgggttct ttttgatcgt aaagaagcac tctgtcgcgc aactgcggag cggtgccttt
     3841 caggtatttg atgatatcgt tgtacacgct cttcgagtcc accacaactc gttccacgtc
     3901 ttcgctgaga aggtcgcgca tgagactcgt cgtcattccg agttctgagt gaatgagcgc
     3961 cggcgccttc attcgtttcg cgcggcgatg gacccgttcc cacgtgcgct tcagatcatc
     4021 gatatcccgc gtcaggtcgt cttcgccctg tccctctgca accgttcgca caatgatgcc
     4081 gcagttttcc ggacgaaggc gcgcggcgag cgctttcagc ctccgttttt ccgaccagct
     4141 cagaatcttg cgggacacgc ccacgtggtt ggaatacggc atcagaacgg agaaacgtcc
     4201 cggcaacgat acgtcggttg tgacacgcgg cccctttgtg ccgatgggct cttttgtgat
     4261 ctgcacgatc acttcctgcc cttttgtcag gagttcctga atcggcgtgt atttttttgt
     4321 ctcgcggcct gatcgcgggg tttcttcgtc gtcgtcctcc tcgtcttcgt cttcttcgtc
     4381 gtcgagccgt tccggagaac caccaacgtc tcttgcgtgc agaaaggcgc ttttttccat
     4441 cccgatatct acaaatgccg cctgcatacc cggcagaatc gatgtgacga ttcccttgta
     4501 aatgttaccg acgaggcgct gcgtatcggg gcgttctacg aggatttcaa ccaggcgtcc
     4561 gtcttcgagg atagcgatac gggtttcctg ggtagcagaa ttgacgatga tctctgtttt
     4621 caacgcgttc cgtctttccg ttcactggaa ccacggacac ggcgacccgt ttccagtggt
     4681 ccggcatcga acgaggttcc gtgatcagac ggacggctgg aaacagagaa cggggagcgg
     4741 cgcgaatgga ccagaatacc ccggcggctg agcagccgga gggcgcatcg ggccgtatcc
     4801 gggccttgaa aggcccctga tcccgatttc acgccgtcga cagggtattc ccgccatcaa
     4861 aacgcgacaa cagaactgcg ctcggagcgc gccgtcgcca caggtacacc aaagcaccgc
     4921 ggcctggtcc cccggcaaaa ccatcattct ttgcgcgcgt ctccagagcc gatacctcga
     4981 tccgtcccgt gccgttttcc agaacccgtt cccgcttgtc gttgcggtca cgtacttcgc
     5041 tcgtcggttg ccgtgcgttc cagttttccg tttaatttgc gatcagcgct acagtgtctg
     5101 gccgcgaaac cgagttgcag gtaaatccgc acggcaaccg cgccgaattt gtttttcgtg
     5161 gcacactgca ctcgggtctg gcgaccgatc gcccgtatct ccacaagtcc ccagaatgcg
     5221 catcggtggc gcagaaggga aagaaaaggc caaaataaag ggt
//
LOCUS       DAOSOO010000214         5213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16003_length_5213_cov_5.485847, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000214 DAOSOO010000000
VERSION     DAOSOO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16003_length_5213_cov_5.485847"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLT86_12385"
     CDS             <1..1239
                     /locus_tag="PLT86_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37451.1"
                     /translation="TPEKSAAAGELFLKERVDVVLVFPFGYTPAMCVVPAARSLSVPI
                     RILNAHEDASYDYAKADTTEYLHHEGVCCVPEYASALRTIGKSFRVRTGAFRDRRLWD
                     EIAADCRGAAAARVFRALNFGVIGETYTNMADMPVDELRLLNATGRLLIRPEVEEIEE
                     AYHHATRAQVDEMCGQFRAMYDVDAGVTDEHLRESARIAVAFDEVIMHHDLSAFGFYW
                     WGVKDAVTQLRAQAALAVSRLAALGRPGVTEGDVKTAMAMKLLDLLGAGGMFVEFFSM
                     DFDENFILMGHDGPSNINVAQDRPKLSFLEVHHGKSGSGLGIDFHMKEGPVTLVNLTQ
                     VDAGESFKLITTAGEVVPGNILSIGNPNCRVRVERPIHEFMDAWCQQGPSHHIALGIG
                     DHTAEVEAFAEAMGFRYVRV"
     gene            complement(1298..2116)
                     /locus_tag="PLT86_12390"
     CDS             complement(1298..2116)
                     /locus_tag="PLT86_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT37452.1"
                     /translation="MALTSRRAEAAKFTLLAGAAFFVLVPFWVMISISLKDLRQFLDN
                     ILYPTSPFQWQNYVEGWEAVSPYIINTLVVAAVVTVVSLTLSSLTAYVFARYEFPGKT
                     PLFYALIVLLMIPGILNLIPQYVLALRLGLVGTAWALILFYVASRQVLEVYILRTFFS
                     GLPKELFEAAQMDGASDIRQYWSIALPLSKSILGTLAIMTVVFVWNDYIWPLVAADNF
                     KTVSIGLAYLTKQHSTSWGPLMAGYAIASLPLILLFSVTMRYFVEGIATGAIKA"
     gene            complement(2161..3090)
                     /locus_tag="PLT86_12395"
     CDS             complement(2161..3090)
                     /locus_tag="PLT86_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT37453.1"
                     /translation="MDNAPRERTSGDGRMPWYYGARLYMYILPTFVFIGVFAYYPSVS
                     AIYHSFFNWNGSNIKDYVGLANFRRIFLNDRDLLVASWHMLLFVSANLVLQIPSILLA
                     VVLYHLRNKRVQYWFRMVFVIPMVVPGMVGMLLWMFIYNPQVGLLNRALAGLGIIPSP
                     FDGPTWLADTRLAMPSLMFMGFPYINPIALLIYLAGLERIPRDLNDSALIDGASAWQR
                     LVRVELPMILGQVKLMLILGIIGGVQDYGRVLIMTGGGPSDATTVPGLIMYNTAFRYA
                     QMGYASAIGVVMFLVILGLTVLNMRYFRTHEAS"
     gene            complement(3210..4658)
                     /locus_tag="PLT86_12400"
     CDS             complement(3210..4658)
                     /locus_tag="PLT86_12400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37454.1"
                     /translation="MPFPHLLRDLKEFFRYRPSVLPGELTPADRQDSLRKLIMAWCLG
                     TVFFEGISGAPLVGMFRSLGATPFWIGLLVAIPSLATLAQPIGSYFVARTGSRKRMLI
                     RTAIPGRLIWLVIPTLGFFFPHNYFVIGVMFTLVLFSRLTIAFASPAWFSWVSDLVPE
                     DERGTFWGSRQMWARIFGIAAAITLGWFLGKEPPYYKFIVYFCLMGFIGWLEIFIHRG
                     VMGIRIPVPEQPPTLAALVLRPLRDRRFLPIIVFTFSINFATQIGGGMFSLMMLEEIK
                     LSYLEISVFQVGILGLVTVLSSRYVGRVIDNLREGARLVFYFGALVTSLQALPWAAIP
                     ERAILPVAGTVILGGVGWAAYHIALTGLLMGYSPRDARATYSAMYAVISGFGSAFGSV
                     LGGTIAEALQGMSYTWGPFILTPLRTLYLISFSGRILALLLLPLIREPASVPLGVYVR
                     QLLSMNPFDRDTYVYIKRKFSPPVRNNGSEQE"
     gene            complement(4799..>5213)
                     /locus_tag="PLT86_12405"
     CDS             complement(4799..>5213)
                     /locus_tag="PLT86_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT37455.1"
                     /translation="ASMGGSACLTFAALHPGLVDGVASMNGTANFLEYEEFPEGLVAI
                     AESFGGTKKEIPLEYKRRSAEFFPERFTMPLGIAASGKDTLVPPGSVLRLANMVKKIG
                     GQVLLIYREETGHTTSYEDGRAIIEFVLDTRTASA"
BASE COUNT         1171 a         1525 c         1612 g          905 t
ORIGIN      
        1 acgccggaaa aatccgccgc cgcgggcgag ctcttcctca aggagcgtgt ggacgtcgtg
       61 ctggtgtttc cgttcggcta cacgccggca atgtgcgttg ttcctgcggc gcgctccctg
      121 agcgtgccga tccgcatcct caacgcccat gaggacgcgt cctacgacta cgccaaagcc
      181 gatacgacgg aatatcttca tcacgagggc gtgtgctgcg tgccggagta cgcctcggcg
      241 ctgcgcacga tcggcaaatc ctttcgcgtg cgcacgggag cctttcgcga caggcggctc
      301 tgggatgaga tcgccgcaga ctgccgcggc gcggccgcgg cgcgggtgtt ccgggccctc
      361 aacttcggag tgatcggcga aacctacacc aatatggcgg atatgccggt cgacgagctg
      421 cgtctgctca atgcaacggg gaggctcctg attcgccccg aagtggagga gatcgaggag
      481 gcatatcacc acgcgacccg cgcgcaggtg gacgagatgt gcgggcagtt ccgcgcgatg
      541 tacgacgtgg atgcgggggt caccgatgaa cacctgcgcg aatcggcgcg catcgcggtg
      601 gcgtttgacg aagtaatcat gcaccacgac ctgtccgcat ttgggttcta ctggtgggga
      661 gtgaaggatg cggtgacgca gcttcgggcc caggccgcgc ttgccgtgtc gcgccttgcc
      721 gcgctgggaa ggcccggcgt caccgagggc gacgtgaaaa ccgcgatggc catgaaactg
      781 ctcgatctgc tcggcgccgg tggtatgttc gtcgagttct tttcgatgga tttcgacgag
      841 aacttcatcc tgatggggca cgacggtccg agcaacatca acgttgcgca ggaccgcccg
      901 aagctttcgt tcctcgaggt gcaccacggg aaatcgggca gcgggctcgg catcgacttc
      961 cacatgaagg aagggccggt gacactggtg aacctgacgc aggtggatgc cggcgagtcg
     1021 ttcaaactca tcacgacagc cggggaagtg gtgccgggga acattctctc aatcggtaac
     1081 ccgaactgcc gcgtgcgggt ggagcggccg attcacgagt tcatggatgc gtggtgccag
     1141 cagggcccgt ctcatcacat cgcactcggc atcggtgatc acacggcgga ggtggaagcc
     1201 ttcgccgagg cgatgggttt ccggtatgtg agggtgtagc gcgcgggtcg tcgagtggcg
     1261 tggcgatagc cgcgttgtat cgagacgacc gccacgctta cgccttgatc gcgcccgtcg
     1321 cgatgccttc gacaaaatag cgcatggtga ccgagaagag gaggatcagg ggaagtgacg
     1381 cgatggcgta ccccgccatc agcggtcccc acgaggtgga gtgctgcttc gtcaggtacg
     1441 caagcccgat ggagacggtt ttgaagttat cagcagccac cagtggccag atgtagtcgt
     1501 tccagacgaa caccaccgtc atgatggcga gcgtgccgag aatggatttg gaaagcggca
     1561 acgcgatgct ccagtattgc cggatgtcgc tcgccccgtc catctgcgcc gcttcgaaca
     1621 gctctttcgg cagccccgaa aagaatgtgc gcaggatgta cacctcaagc acctggcgcg
     1681 atgcgacgta gaacaggatc agcgcccacg cggtgcctac aaggccaagc ctcagcgcaa
     1741 gcacgtactg gggaatgagg ttgagaatgc cgggaatcat cagaagcacg atcaaggcgt
     1801 agaacagcgg cgttttgccg gggaactcat accgcgcgaa cacatacgcc gtcagcgaac
     1861 tcaacgtgag acttaccacc gtcactaccg ccgcgacgac aagagtgttg atgatatacg
     1921 gggacaccgc ctcccagcct tccacgtaat tctgccactg gaacggggaa gtcgggtaaa
     1981 ggatgttatc gaggaactgg cgcaggtcct tcagactgat gctgatcatc acccagaacg
     2041 gcacaagcac aaaaaacgcc gcacctgcca gcaatgtgaa ctttgcggct tccgcacgcc
     2101 tgctagtcaa tgccatactg agcctttgcc gcgttcactt ccagaccacg cgtctacctc
     2161 tcatgacgcc tcgtgcgtgc ggaaataccg catgttgagc accgtcaatc cgagaatcac
     2221 aagaaacatc accacgccga tggccgacgc atagcccatc tgtgcgtacc ggaaggcggt
     2281 gttgtacatg atgagccccg ggaccgtggt ggcatcggag gggccgcctc ccgtcatgat
     2341 gagcacccgt ccgtaatcct gcacaccccc gataatgccg agaatcagca tcagtttcac
     2401 ctgcccgagg atcatcggca gttcgacccg cacaagccgc tgccacgcgc ttgcgccgtc
     2461 aatcagcgcg gaatcgttca ggtccctggg tattcgttcc aaccccgcga gatagatgag
     2521 aagggcgatg gggttgatat acgggaaccc catgaacatg agacttggca tggcaaggcg
     2581 cgtgtccgcc agccaggtcg gaccgtcgaa tggcgaagga atgattccga gcccggccag
     2641 cgcccggttc aaaaggccca cctgcgggtt ataaatgaac atccacagga gcatgcccac
     2701 cataccgggc accaccatcg gtatcacgaa taccatccgg aaccagtact gcacgcgctt
     2761 gttccgcagg tggtacagga ctacggcaag cagaattgac ggaatctgca ggacaaggtt
     2821 cgccgagacg aacagaagca tgtgccacga ggcaaccaga aggtcccggt cgttcaggaa
     2881 gattcggcgg aaattcgcaa ggccgacgta gtcctttatg ttcgaaccgt tccagttgaa
     2941 aaacgagtgg tagatggcac tgacggaggg gtaatacgcg aacactccga tgaagacaaa
     3001 cgtcggcagg atgtacatat acagccgcgc gccgtaatac cacggcatgc gtccgtcgcc
     3061 ggacgtacgc tcgcgaggag cattgtccat cgagaggacc gtttctgaaa gaggatggcc
     3121 tgctttgtgt gcgcaaagaa atatggcagg actgcgttcc cactgacaag gcaaggagga
     3181 ttgcgattga ggggaaaccg gagagaagct tactcctgct ccgatccgtt gttccggacg
     3241 ggaggactga atttccgttt gatgtagacg tacgtatcgc ggtcgaaggg gttcatggaa
     3301 agaagctgcc ggacgtacac gcccagagga accgaggcgg gctcacgtat caggggcaac
     3361 aacaagagcg ccagaatacg tccgctgaag gagatgaggt agagcgttcg gaggggtgtc
     3421 agaatgaatg gcccccaggt gtacgacatt ccctgcagtg cttccgcgat ggttcccccg
     3481 agaaccgagc cgaacgcgct tccgaaccct gaaatgaccg catacatcgc gctgtacgtc
     3541 gcccgcgcat cccgcggcga ataccccatg agcagcccgg tgagcgcgat atggtacgcc
     3601 gcccagccga caccccccag aatcaccgtt cctgcaacag ggagaatcgc ccgctcgggg
     3661 atggccgccc agggaagcgc ctgcagcgat gtgaccagcg ctccgaaata gaacacgagc
     3721 ctcgctccct cgcgaaggtt gtcaatcacc cgcccgacat aacgcgaact gagcaccgtc
     3781 accagtccca ggatacccac ttgaaacacg gatatctcga ggtaggatag tttgatctcc
     3841 tccagcatca tgaggctgaa cattccgccg ccgatctgcg tcgcgaagtt gatcgaaaag
     3901 gtgaacacga tgatgggaag aaagcgacgg tccctgagcg ggcgcagaac aagcgctgcc
     3961 agagtcggtg gttgctccgg taccggtatc cggattccca tcaccccgcg atggatgaag
     4021 atttccagcc agccgatgaa tcccatgagg cagaaataga cgatgaactt gtaatacggc
     4081 ggctctttcc cgaggaacca tccaagcgtg atcgccgcgg cgatgccgaa aatgcgtgcc
     4141 cacatctggc ggcttcccca gaacgttcca cgctcgtcct cgggcacaag gtcgctcacc
     4201 catgaaaacc aggccggcga ggcgaatgca atcgtaagcc gcgagaacag caccagggtg
     4261 aacatcaccc cgatgacgaa gtaattgtgg ggaaaaaaga agccgagtgt cgggatgaca
     4321 agccagataa ggcggccggg gatggcagtg cggatgagca tacgcttccg ggaaccggtg
     4381 cgcgccacga aatacgaacc gatgggttgc gcaagagtcg cgagggacgg aatggcgacg
     4441 agcaggccga tccagaacgg cgtcgcaccg aggctgcgga acattccgac aagcggcgcg
     4501 ccgctgatac cctcgaagaa caccgttccc agacaccacg ccatgatgag cttgcgcagc
     4561 gaatcctgcc tgtcagccgg cgtcaactca cccggaagaa cggagggccg gtaacggaag
     4621 aattccttga ggtcacggag gaggtgtgga aacggcaagg tcgaacttca gcctgcggtt
     4681 accctgggtg ggcgggtgga agaaacctgg aacgatggac tattcaaagt ttaataaacg
     4741 ggatggcggg gacaaagtgg ctattccggt ccctcttgtg gagcagaacc cacgagaatc
     4801 aggcggaagc cgtgcgcgta tccagtacga actcgatgat cgcccggcca tcttcgtagc
     4861 tggtggtgtg accggtctct tcccggtaga tgagcagcac ctgcccgccg attttcttca
     4921 ccatgttcgc aagccgcagg acactcccgg gcgggaccag cgtgtccttt cccgaggcgg
     4981 caatccccag cggcatggta aaccgctcgg ggaagaactc tgcgctgcgc cgtttgtact
     5041 cgaggggaat ctcctttttc gtgccgccga atgactccgc tatggcgacc aacccttcag
     5101 ggaactcctc atactcgagg aaattcgccg tgccgttcat gctggccacg ccgtctacca
     5161 gcccggggtg gagcgcggcg aacgtcaggc atgcgctgcc gcccatggag gcg
//
LOCUS       DAOSOO010000215         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16268_length_5152_cov_5.630763, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000215 DAOSOO010000000
VERSION     DAOSOO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16268_length_5152_cov_5.630763"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PLT86_12410"
     CDS             complement(<1..1888)
                     /locus_tag="PLT86_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOT37456.1"
                     /translation="MSKTDAPPGGAAKSESAPARSLILIGNPNVGKSAIFGHLTGVYV
                     VVSNYPGTTVEVTRGVAQSKEYEIYDTPGANGLVPMSEDELVTRSILLEQPDAPVVQV
                     ADMTNLRRALILTSQLSDWERTGALALNMADEAASRGIHVDLKSVEKLVGVPVRATVA
                     VRRSGVREIAERSFEGRPFQLRIEFQEAIEKAISDITALLPKDMRGKRGVALTVLSGD
                     ETIFPWLRNQTSQEVVQRLEAIREHVRVQFPGGVAAVINTERLRRIDAIVAQVMRIRD
                     TAGNHVLALVSSWATHPLKGLVVVGAVLYVTLWFVGLFGAGTLVDFFETFVFSQFVSP
                     FFIRLADAILPFPHSHVIEPVKWTLSLPLSALHQVDLFTVARSVPSTTYAVTDGVSLT
                     WWQTTARVVHDFFVGPYGMITMALAYGFAIVLPIVATFFIIFGLLEDSGYLPRLAVMT
                     NRLFRMIGLNGKAVLPMVLGFGCDTMATMTARILETKKERVITTLLLALGVPCSAQLG
                     VILAMVATISLTGVLWWVITVAVVMMLVGWLAARVLPGDSSDLVLELPPMRVPQFGNI
                     IVKTIARIEWYLKEVVPLFVLGTALLFFLDLFDALGAIRSFAAPVVQGWLGLPAQATE
                     AFLIG"
     gene            2151..3299
                     /locus_tag="PLT86_12415"
     CDS             2151..3299
                     /locus_tag="PLT86_12415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT37457.1"
                     /translation="MEGPRACRPEELDGLRTLENTVFRSNGGSCMFREFPTLFHESNC
                     ERLRVFVDKGQPVSAIAYVSRTAVIQGNTLRIGSLGAVATFEEYRGRGLATALLEDTF
                     AQMKRENVDVVLISGQRGLYQRAGCAIAGHEMRFSLDGSALAGAPERGLVVEEAADAD
                     ISDLIALHQNEPSRFVRSPWEWQQFLSVYRLTPKGAEPPFGVRRLWVVRQQGTPVAYF
                     VLSVGRDNTNPTAYVQEFAGNRAAVLSGLTSLKERFGLRTVSGSCLPSDREMNGLLTG
                     LGGSVQASSIGGHRMSFLNPEVLRRYDPWVTERLGMEFVGELNLVQSDDVWYLTSGAT
                     RTVAGSLETTNALLWGDAVRDLKGDDTDTGVWKRVLPLPFLLPGMNYI"
     gene            3325..4464
                     /locus_tag="PLT86_12420"
     CDS             3325..4464
                     /locus_tag="PLT86_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37458.1"
                     /translation="MEPHERVLVVLLKSTTDWDIARDRNWYRIPAAHAPKDPGAEWIA
                     FYQPAGFGRERWGVYSYARVRGTHTVRRIELFPSETDHPRAHEAYLVYSFDPPARLAH
                     PLQCARPRRFVWTMTTWWRFSNARTFDDLLRRDPLLPPEKDTVLVSVVPRVSDFEIVT
                     KKHWYRIPCSVVSQWATPAYLAFYHGQGFGVEAGQVRYFAPIRYVDIVKRVDLFPDEP
                     KHPRARADYYRVHIDDLQQRPSPIRSKRKRQVVLIPTTFERFMLAGDINELAVGDEAT
                     DSFYGRMTEEGLRPERAYHVRGSNAYYFTDFAILGRKGSVQVDVARGENQNGFLPKRA
                     GRHAETPDGWTALRLSRFDITKRREDAIERIRNAVAAGAGSVMVR"
     gene            complement(4499..>5152)
                     /locus_tag="PLT86_12425"
     CDS             complement(4499..>5152)
                     /locus_tag="PLT86_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT37459.1"
                     /translation="NAILGEDRAIVSHIPGTTRDLVEGEADLDSVRVRFVDTAGLRET
                     GDAVEREGTKRAGRAASAADVVLLVCDGSGEIPWDLTPVSGRNAIIVINKMDKARNEV
                     LAALCASAGGTPVFRVSALSGEGVDTLCRGIRDALCADAPEPKTGGVIIRARHADALS
                     AAGSHLERALDEATGQARSELVAADIRLALDALGSITGETTPEDIIRRIFSEFCVGK"
BASE COUNT         1104 a         1508 c         1547 g          993 t
ORIGIN      
        1 agcctatcaa aaatgcctcg gtcgcctgag cagggaggcc cagccagcct tgaacaactg
       61 gcgcagcgaa acttcggatc gcacccagag catcaaacaa atccaggaag aacagaagtg
      121 cggtaccgag aacgaacaaa ggcaccacct ctttgagata ccattcaatc ctggcaatcg
      181 tcttcacgat gatgttccca aactgcggta cgcgcatggg cgggagttcc aacacgagat
      241 cactggagtc acccggcaga actctggccg ccagccagcc aacgagcatc atgacgacag
      301 caaccgtaat gacccaccac aatacccccg tcaggctgat ggtcgccacc atcgcgagaa
      361 tcactccgag ttgcgccgaa cagggtactc ccagggcaag gaggagcgta gtaatgacgc
      421 gttccttctt cgtttcaaga atccgcgccg tcatggtggc catcgtatcg catccgaaac
      481 ccagaaccat gggaagcacg gccttaccgt tcagccctat catgcgaaac aggcgatttg
      541 tcatcacggc gagacgcggg aggtaccctg agtcttcgag aagaccgaat atgatgaaaa
      601 acgttgccac gatgggcaga acaatcgcga acccgtaggc gagggccata gtgatcatgc
      661 cgtacggccc gacaaagaag tcatgcacga cgcgcgccgt cgtctgccac caggtgagag
      721 acacgccgtc tgtaacggcg tacgtggtag acggaacact cctcgctacg gtgaacaagt
      781 ccacttgatg aagcgcggac aacgggaggg acagggtcca cttcaccggt tcgatgacat
      841 gcgagtgagg gaacggcagg atggcatccg ccagacggat gaaaaacgga ctgacgaact
      901 gcgagaatac gaacgtttcg aagaaatcca ccaaggtgcc cgcgccgaac agccctacaa
      961 accagagagt cacgtacaga actgctccga cgacgaccag ccctttcagc ggatgcgtcg
     1021 cccatgagga gaccagggcg agaacatggt tgccggcagt atcacgaatc cgcatcacct
     1081 gcgcgacgat ggcgtcgatt cttctcaatc gttcggtgtt gatgaccgca gctacgccgc
     1141 cgggaaactg gacacggaca tgttcccgaa tggcttcaag gcgctgaaca acctcctggg
     1201 aggtctggtt cctcaaccac ggaaaaatgg tctcatcccc ggagagaacc gtcagtgcga
     1261 cgccacgttt gccccgcata tctttcggaa gcagggcggt gatgtcagaa attgcctttt
     1321 caatcgcctc ctggaattcg atcctgagct ggaagggacg cccctcgaaa ctccgctctg
     1381 caatttcacg aacaccgctc cgcctcactg cgaccgtggc ccggacgggg actcccacca
     1441 gtttttccac ggatttgagg tcaacatgga ttcctctgct ggccgcctca tccgccatgt
     1501 tgagcgcaag cgcaccggtt ctctcccagt ccgaaagctg tgacgtcaga atgagcgccc
     1561 gccgaaggtt tgtcatgtcg gcgacctgaa caaccggcgc atcgggctgt tcgagaagaa
     1621 tcgagcgcgt gaccagttcg tcttcggaca tcggcacaag cccgtttgcg cccggtgtgt
     1681 cgtagatctc gtattccttc gactgcgcaa ctcccctcgt gacctcgacg gtggtgccgg
     1741 ggtagttgga tacgacgacg tacactcccg tcaagtgacc gaatatggcg cttttgccga
     1801 cgttagggtt gccgatgagg atgagggagc gagcaggagc agattcgctt tttgcggccc
     1861 cgccaggggg agcgtccgtc ttgctcattt cacatccttc gggagacgaa tgcaggatgg
     1921 tgagtggata agaagccgtt cgcatcgctt cgtactggaa tataacttaa ttgagattca
     1981 ttctcaattc cgttccaact tcacgagtcg cattctcaga aagaacgcgg catgcgccat
     2041 gccgcggaga ggcgtggctg gcgtacaatc gcaaacgcat ctaaattcaa acctgaaaaa
     2101 gggaagggaa gccctgcctg cacacaacac ccggatagat cgaggtgccc atggaaggtc
     2161 cgcgcgcgtg tcgccccgaa gagcttgatg gcctgcgtac tctcgaaaac accgtctttc
     2221 gctcgaacgg gggaagctgc atgtttcgag aattccccac gctcttccac gaatccaatt
     2281 gcgagaggtt gcgtgttttc gtcgacaagg ggcaacccgt ttcggccatt gcctacgtca
     2341 gcagaacggc agtcattcaa gggaacacgc tgagaatcgg ctcgctgggt gctgttgcga
     2401 cattcgagga gtaccgggga cgcgggctgg cgaccgccct ccttgaggac acgttcgcgc
     2461 agatgaaacg tgagaacgtt gacgttgtcc tgatctcggg acaacgggga ttgtatcaga
     2521 gagcggggtg cgccatcgcg ggacatgaga tgcgtttctc gctcgatgga tcggcactgg
     2581 ccggggcacc ggaaagagga ttggtcgtcg aggaagctgc ggatgcggac atttccgacc
     2641 tgatcgcgct gcatcagaac gaaccttcgc ggtttgtccg gtcaccctgg gagtggcagc
     2701 agttcctgtc ggtgtaccgt ctcaccccaa aaggagcgga acctccgttc ggggttcgcc
     2761 gtctctgggt tgtccgccag cagggcacgc cggttgcgta tttcgttctt tccgtgggac
     2821 gagacaacac caacccgacc gcgtatgtcc aggaatttgc cggaaaccgt gcagcagtgc
     2881 tgtccgggct cacctcgctg aaggagcggt tcgggctgcg gacggtgagt ggaagttgcc
     2941 tcccctccga ccgcgagatg aacggtttgc tcaccggtct cggcggatcg gtgcaggcat
     3001 ccagcatcgg cggccatcgg atgtcttttc tgaatccgga ggttcttcgt cggtacgacc
     3061 cgtgggtgac ggaacgactg ggtatggagt tcgtgggcga actgaacctt gtccagtcgg
     3121 acgatgtctg gtatctgacg tccggcgcca ccagaaccgt tgcgggttcg ttggaaacca
     3181 cgaatgcgtt gctctggggc gatgccgtga gggatctgaa aggggacgac acggacaccg
     3241 gagtgtggaa acgggtgttg ccgttgccgt tcctgctacc cggaatgaac tacatctgaa
     3301 gctgcatctg gacgatcggg gcggatggaa ccacacgaac gcgtgctcgt ggtgctcctg
     3361 aaaagcacca ccgactggga catcgctcga gaccgcaact ggtaccgtat tccggctgcg
     3421 catgctccga aggacccggg cgccgaatgg attgcgttct accagcctgc gggttttggc
     3481 agggagcgat ggggtgtgta ctcctacgca cgtgttcgag gaacgcacac ggtgcggaga
     3541 atcgagctgt ttccctcgga gacagaccac ccccgtgcgc atgaagcgta tctggtgtac
     3601 tcgttcgatc ctcccgcacg gctggctcac ccgcttcaat gcgcacgacc ccggcgtttt
     3661 gtctggacca tgaccacgtg gtggcgtttc tccaacgccc gcacgtttga cgatctcctg
     3721 aggcgcgacc ccctgttgcc cccggagaag gacacggtcc ttgtgagtgt tgtcccacgc
     3781 gtgagcgact tcgagatcgt cacgaagaag cactggtacc gaataccctg ttcggtcgtg
     3841 tcgcagtggg ctacgccggc gtacctcgcg ttctatcatg ggcaggggtt cggcgtggaa
     3901 gcaggccagg tgaggtattt tgcgcccatc cgttacgtag acatcgtcaa gagggtggat
     3961 ctgtttccgg atgagcccaa gcacccccgt gccagggcgg actactatcg cgttcacatc
     4021 gatgacctgc aacagcgccc gtcacccatt cgcagcaaaa gaaaacgaca ggtggtgctg
     4081 attccgacga cgtttgagcg tttcatgctg gcaggagata tcaacgaact tgccgtggga
     4141 gacgaggcga ccgactcgtt ctacggcagg atgactgagg aggggttgag gcccgagaga
     4201 gcataccacg tacgaggcag caacgcgtac tacttcactg atttcgcgat cctcggccgc
     4261 aaggggagcg tgcaggtgga cgtcgcgcga ggggagaatc agaatgggtt ccttcccaaa
     4321 agggccggga ggcacgccga aacacccgat ggctggaccg cgttgcgcct gtcccgtttc
     4381 gatatcacga aacggcggga ggacgcaatt gaacggatca ggaatgccgt ggcagcagga
     4441 gcaggaagcg tgatggttcg ctgacaagag gaatacgccg cgcaacgctc ccggacacct
     4501 atttgccgac gcagaactcc gagaagatgc ggcggatgat gtcttcgggt gttgtctccc
     4561 ccgtgatact acccagggca tccagggcca ggcgaatgtc cgctgcgacc agttcggagc
     4621 gcgcttgacc ggtggcctcg tcaagggcgc gttccagatg gctcccggct gcggaaagag
     4681 cgtccgcatg tcgcgcacgg atgatgactc cccctgtttt cggctccggg gcatccgcgc
     4741 acagagcatc gcgaatccca cggcaaaggg tatccacgcc ttcaccggac agcgcgctga
     4801 cacgaaagac cggtgtgcct cccgcggaag cacagagcgc ggccaacacc tcgttgcgcg
     4861 ctttgtccat tttgtttatg acgatgatgg cgtttctgcc cgaaacgggc gttaggtccc
     4921 acggtatttc gcctgacccg tcgcatacca gcagcacgac gtcggcagcg gaagcggccc
     4981 ttccggccct cttcgtgcct tccctttcga cggcgtcccc ggtttccctc aaccccgcgg
     5041 tatctacaaa tcggacccga acactatcga ggtcggcttc gccttcgacc agatcgcgtg
     5101 tggtacccgg gatatgtgat acaatagccc ggtcctcccc gagtatcgca tt
//
LOCUS       DAOSOO010000216         5135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16355_length_5135_cov_5.527756, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000216 DAOSOO010000000
VERSION     DAOSOO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16355_length_5135_cov_5.527756"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLT86_12430"
     CDS             <1..463
                     /locus_tag="PLT86_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37460.1"
                     /translation="EDGGIPEVRICVESANCWSQGYHYITIECADGTIEINKPGSPPV
                     IAKGWRSAEEELKELQEEPDPSTLAVRDLFDAVERHREPVSNGITGYKAVEFILAIYQ
                     SALKRSIVELPLPLDTPDPLRHFFNWDGVIRGGRGTAFSTTGAVTTTGKIS"
     gene            complement(456..2357)
                     /gene="asnB"
                     /locus_tag="PLT86_12435"
     CDS             complement(456..2357)
                     /gene="asnB"
                     /locus_tag="PLT86_12435"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOT37461.1"
                     /translation="MCGIAGIWHRDGRPVSPQMISAMTDVQSHRGPDDRGTYVSGDLA
                     LGHRRLAIIDLTETGHQPMSNRDGTLWITYNGEVYNYVELRAELEGRGARFRSTSDTE
                     VLIAAYEQWGESCVERFNGIFSFAIWDSRRQTLFCARDRFGVKPFHFAEIGPTFLFAS
                     EPKGVLASGIVPRGPDWRVVYRYLVLNLLNVDNNTFFSSIQKLPPAHTLTVHRDGGSV
                     LRRYWDIPTIQSGEQRSDAEWLEEYEATLEDSVRLQMRSDVPVGVCLSGGLDSTSITR
                     IATEFSSIPTKTFTAFLPHPLYDEREPVSAFTRLMNGKTEPHFVSPELDTWVHELPKV
                     VYHHDEPHAGYIAATQWEVMRLVHQRGVKVVLDGQGSDETLGGYNFFVESAAADLVRS
                     GRVLDAFRVLSLLEDRRGTGYAAGARRLIKVAGRAFFSRDHLFEIEGKWNAAGLPLSR
                     EWCSRFRRFDIPTIETTGSRLEDDQRTAMFVTMLPYLLTFEDRASMAFSIESRVPFLD
                     HRLVETALRLPRHLRVNGTTTKVALRRIMEGKLPGEIVNARLKRVFGTPYEHWFSEKL
                     SGFARDILTSQAFLERPFIEKRKVPGLIASFAAAARSRRKAFNIWNLINLELWFRVCV
                     EQNADIVPN"
     gene            2691..3935
                     /locus_tag="PLT86_12440"
     CDS             2691..3935
                     /locus_tag="PLT86_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT37462.1"
                     /translation="MRKWFWSGIVAITAAAANVGAAGFALYEHGAAATATAGAFIARA
                     NDPSAIYYNPAGISGQRAGVMLGTTLITPSGDFAGANPFPGTGYTAKQKDALFFPSHL
                     YVASPLGPVTVGLGVFNPFGLGTNWPEDWKGRDISTKTEIQTYYFNPVVAYSVGPVSM
                     AAGMQAVYGSLELNRKVKHPLLGDDVAAMHLEGTSDINYGFNAGVQFRPMPMLTVGAS
                     YRSQVDSKVNGTAAFDSMNAKWTMVKTSLPAKADVSTTMPYPAVMGIGVAVTPIPDLT
                     IEANVVQTQWSTFDTLTIAFAAPYSALTEKTPELYSNARSYRIGAEYRLPAWSLALRC
                     GYLFDESPAPPMAVTPLLPDADRDSFQVGAGYTMGKTTFDFAYMYLKFKERSTEGLNP
                     KNFNGTYNTTANLFGFSVTHRF"
     gene            complement(4382..>5135)
                     /locus_tag="PLT86_12445"
     CDS             complement(4382..>5135)
                     /locus_tag="PLT86_12445"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT37463.1"
                     /translation="NWVYVAGHVVPEGVEAAVNAVRGLCLPGMNITVPHKKAVIQFLD
                     ELLPAAKAVGAVNTIVNKDGQLLGDNTDVYGILQAIRVGAKMDTVPPVVVVLGAGGAA
                     RGIVYALTTLPEVKSIRILNRTLANAEELAREFGATSSVTAFGLEPHAARHALDGCGL
                     LINATNLGRGKLANISPLPDAWNCIPPRIVCIDSNYSPATTRLMEQVVAAGGVAFNGL
                     DMLVHQGARAFELWTGMMPPVEVMRAAANARL"
BASE COUNT         1107 a         1457 c         1482 g         1089 t
ORIGIN      
        1 cgaagacggc ggaattcccg aggtccgcat ttgcgttgag agcgccaact gctggtcgca
       61 gggctaccat tatatcacca tcgaatgtgc cgacgggaca atcgaaatca acaagccagg
      121 atctccgcca gtgatcgcca aaggatggcg gagtgctgaa gaagaactga aggagctgca
      181 agaggagcct gacccttcaa cgctggcagt gcgggatctc tttgatgctg tggagcgcca
      241 ccgtgagccc gtttcgaacg gaataaccgg gtacaaagct gtggaattca tcctggccat
      301 ttaccagtcg gcattgaagc ggtccatagt ggaattgccg cttcccttgg acacgccgga
      361 cccgttgcga cactttttca actgggatgg ggtgattcgt ggcggcaggg ggacggcatt
      421 cagtactacc ggggccgtca ctaccacagg aaaaatcagt tagggacaat gtccgcgttc
      481 tgctccacac agacgcggaa ccacaactcc aggttgatca gattccagat gttgaacgcc
      541 tttcttctgc tccgtgccgc agcagcgaat gaggcgatca atcccgggac tttgcgtttc
      601 tcgatgaatg gccgttccag gaaggcctgg ctcgtcagaa tgtcgcgtgc gaaaccggag
      661 agcttctccg aaaaccagtg ttcgtaagga gtgccgaaaa cccttttcaa tcgggcattc
      721 acgatctctc cgggaagctt tccctccatg atccggcgaa gtgcgacctt tgttgtcgtt
      781 ccgttcactc tgagatgtcg tggcaatctg agggcggtct ctacgaggcg gtggtcgaga
      841 aaaggaacgc gcgattcaat ggagaacgcc atgcttgcac ggtcctcgaa tgtgaggaga
      901 tacggaagca tagtgacgaa catggccgtg cgctgatcgt cttccaatcg cgatccagtt
      961 gtttcgattg tggggatatc gaagcgacgg aaccgggagc accattcccg cgatagcggc
     1021 aaaccagcgg cgttccattt cccctcgatt tcaaaaagat ggtcccgact gaagaatgca
     1081 cgtcccgcca ctttgatcaa ccgccgtgcg ccggcggcat agcccgtccc tctccggtct
     1141 tcaagcaaac tgaggacgcg aaacgcatcc agaacccgac cggaacgcac cagatcggcc
     1201 gcagcgctct cgacgaaaaa attgtacccg ccaagggtct catctgatcc ctgaccatcc
     1261 agaactacct tgacgccacg ctggtggacg agcctcataa cctcccactg ggtggcggct
     1321 atgtaccccg catgcggttc gtcgtgatgg tagaccactt tcggcagttc gtgcacccag
     1381 gtgtccaatt caggggaaac gaaatgaggc tcagtttttc cgttcatcaa cctggtgaat
     1441 gcgctcaccg gttcgcgttc gtcgtacaac gggtgtggca gaaacgcagt gaacgttttg
     1501 gtgggtatgg aagagaactc agtcgcaatt cgtgtgatgg aggtggaatc cagaccgccg
     1561 gaaaggcaca caccgactgg aacgtcactc cgcatctgca ggcggacgga gtcttctagc
     1621 gttgcttcgt attcctcgag ccattcggcg tcgcttcgct gttctccgga ttgtattgtg
     1681 ggaatatccc agtaccgtcg tagcacgctc ccgccgtcgc ggtgaacggt cagcgtatgt
     1741 gcgggcggca gtttctggat ggaggaaaag aacgtgttgt tgtcgacgtt cagcagattc
     1801 agcaccagat accggtacac gaccctccag tcgggccccc gcggaacgat gcctgacgca
     1861 agcaccccct tcggttcgga cgcaaacaga aaggtcggtc cgatttcggc gaagtgaaag
     1921 ggttttacgc caaagcgatc ccgggcgcag aaaagtgtct ggcggcgaga atcccagatg
     1981 gcgaacgaga aaattccgtt gaaacgttct acacaggact ctccccactg ctcgtatgcc
     2041 gcgatcagaa cctcggtgtc actggttgat ctgaagcgag cgccccgccc ctccagctca
     2101 gcgcgcaact cgacgtaatt gtacacttct ccgttgtagg ttatccacag agtgccgtcc
     2161 cggttgctca tgggttggtg gccggtttcg gtaagatcga tgatggcaag gcggcgatgt
     2221 ccgagcgcaa gatcacccga gacgtacgtg cccctgtcgt cggggccgcg atgtgactgg
     2281 acgtcggtca tcgcgcttat catctgaggc gaaaccgggc gcccatccct atgccagatt
     2341 cccgcaatac cacacattgg cagctcgatt cggcggagtc gttggcctcg gcttcaagag
     2401 cgcggccgag aaccgaagtg tgggataaat ataccaacca cgtgagttcg ttccggcgtg
     2461 ggagtgtccc acaggctgca gctcggtgca gcgctgcccc tctctcccgc gtcgctgctc
     2521 ccgtctgcag tcacagcgaa cttcgcgccg ggaatcctgt cttgcaaaag cgtatcgcat
     2581 tccttacctt tttctgctca cgggagaatg ccccgtgtgt cgtcggccgg cggttttccg
     2641 gcatccagtc aacctttttc cctgccacat gtgcgggagg aggatcagca atgcgcaaat
     2701 ggttctggtc gggaattgtt gcgattactg cggcggccgc caacgtcggc gcagcaggct
     2761 tcgctcttta cgagcacgga gccgctgcaa cggcgaccgc gggagcgttc atcgctcgcg
     2821 caaacgatcc gagtgcgatt tactacaatc cggcgggcat ttccggtcaa cgcgccggcg
     2881 tgatgctggg tacgacactt attacaccga gcggagattt cgcaggcgca aatccatttc
     2941 ctggtacggg ctacaccgcc aagcaaaaag atgccctctt ctttccttcg cacctctatg
     3001 ttgcatcccc cttgggtccc gtaacggtcg ggttgggcgt gtttaacccg ttcggacttg
     3061 gaaccaactg gccggaggat tggaaaggcc gggacatctc gacaaaaacg gagatccaga
     3121 cgtactactt caatccggtg gtcgcgtatt cggtgggtcc cgtctccatg gcagcaggga
     3181 tgcaggcagt gtatgggtcc ctcgaactga atcggaaagt gaagcacccg ctcctcggcg
     3241 atgatgttgc ggctatgcat cttgaaggca cgagcgacat caactatggc ttcaacgccg
     3301 gcgttcaatt ccgcccgatg ccgatgctca ccgtcggtgc atcgtacaga agtcaggtgg
     3361 attcgaaggt taacggaacg gctgcgtttg acagtatgaa cgcgaaatgg acgatggtaa
     3421 aaaccagcct gcccgcgaag gctgatgtca gcacgacgat gccgtacccg gccgtcatgg
     3481 gtatcggtgt cgcggtcact ccgattccgg accttacgat tgaggcaaac gttgtccaga
     3541 cgcagtggag cacgtttgac acgcttacta tcgcgtttgc ggcgccttac agtgcgctga
     3601 cagagaaaac tcccgagctg tactccaatg cgcgaagcta tcgaatcggc gcggaataca
     3661 gattgcctgc gtggtcgctg gcgttgcgtt gcgggtacct gtttgacgaa tctccggcac
     3721 ccccgatggc agttaccccg cttctgccgg acgctgatcg tgactccttc caggtgggtg
     3781 cagggtacac aatggggaag acgacgttcg acttcgcgta catgtatctg aaattcaagg
     3841 aacgttcgac ggaaggtctg aaccccaaga acttcaacgg aacgtacaat accaccgcga
     3901 atttgttcgg tttttctgta acgcaccgat tctgatcgaa acttgatcgg gggacgccct
     3961 ttcaggcaat gaagtgactg gaagggctga atgagaggcc atggaaacgt ggccggaggc
     4021 gcgggggata cgtcctccgc gctttctgtt tccgggcctg agatttgtct gatgacaggt
     4081 tacgattgct cgaggcggag gaagaggaac ggaaactctt ggatcagaga atgagcagag
     4141 tagaggggcc gccgggatga aacgcttcag atcagggaaa aactgttgaa ggctttttga
     4201 tgctgccctg cagagatata gtatcaccac cccgtaccga ggcggtgagt ccgcaccgca
     4261 ggggagcccc cttcggtaga cgtgtgttga aggttcaata aaacggcaac ccctccaccc
     4321 atggaatatg attgccctgc tcgtgtctgt caatacccgc cgcaaaacgt gatggagttc
     4381 cctataaacg ggcgttggcg gcagcccgca tcacttccac aggaggcatc atccccgtcc
     4441 acaactcaaa agcgcgcgcg ccctggtgca ccagcatatc gagtccgttg aaagcaactc
     4501 ccccggcggc gacaacctgt tccatcagcc gtgtggttgc ggggctgtaa tttgagtcga
     4561 tgcacacgat gcgcggtgga atgcagttcc acgcgtcagg cagagggctg atgttcgcca
     4621 gcttaccgcg cccgaggttt gttgcgttaa tcagcaagcc gcaaccatca agagcgtgcc
     4681 gcgccgcgtg aggttcgagg ccgaacgcag ttaccgagga ggttgctcca aattcgcgcg
     4741 ccaattcctc tgcgttcgcc agggttcgat tgagaatccg tatgctcttc acttccggga
     4801 gagtagtaag cgcgtacaca atccctcgcg ccgccccacc ggctcccaga acaacaacaa
     4861 cgggcggaac ggtgtccatt ttcgctccca cccggatggc ctgaaggatt ccgtacacat
     4921 cggtgttgtc gccgagaagt tgtccgtctt tgttgacgat tgtattgacc gctcccactg
     4981 cttttgcagc gggtaacaat tcgtccagga attggatgac agcctttttg tgggggactg
     5041 tgatgttcat ccctggcaga cacagaccac ggacggcatt tactgctgct tccacgcctt
     5101 ccggaacaac atgccccgca acatacaccc agttc
//
LOCUS       DAOSOO010000217         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16402_length_5126_cov_6.326760, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000217 DAOSOO010000000
VERSION     DAOSOO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16402_length_5126_cov_6.326760"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLT86_12450"
     CDS             complement(<1..335)
                     /locus_tag="PLT86_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37464.1"
                     /translation="MNAVRVPQSVVGIIGRGATVTLRSPDTIRVEPAAVPDGLRPREA
                     TTRGTGEVPVHFAASRRIEKGMLRLRVTNETTGDTGEISLPVRVWGPRMSFICLVQSS
                     NTHVGWDPRP"
     gene            complement(514..587)
                     /locus_tag="PLT86_12455"
     tRNA            complement(514..587)
                     /locus_tag="PLT86_12455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(553..555),aa:Gln,seq:ctg)
     gene            768..2363
                     /locus_tag="PLT86_12460"
     CDS             768..2363
                     /locus_tag="PLT86_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT37465.1"
                     /translation="MAKVLVIDDSEPLLDYYGKILKDIGCEVLTTSLGEEGIRLAREH
                     APDCIMLDIMMSDMDGLDVLQELADFDSTIPVIVVTGMPSAESAIEALKRGAFDFLTK
                     GCTVEEIIATARRALDRRRLYLENQELVAKLREANSRLEQQISEATEQVRELNNFNQS
                     LLEGIDAGLLAASESGVILFANAAARRMLSLSPHQVIGSNLRDFGFEVTRAAHTVRRS
                     ETPHIVNGHFEDSLDSVEKKLERVQKRTTYRLPDGSVRVFGYSVSTTRDDDPTGSGRR
                     SIVLFRDMTDMEELRNQMQRLQKLEALNVVVAGVAHEIKNPIAGIKSVASILVETMKE
                     DDPLRDHVLRILEESRRVTRLVEEFFSFSRPSKPKLEAADVSDIIGRVVRLTHDAATQ
                     RRADIATVVALDIPRVPMDKDQIQQVILNLVLNALEAIGQNGRIEISADVVDYHVLGR
                     RCARVQVRDSGPGFPADVFHRLFDPFFTTKPSGTGLGLHISQNIVLEHGGRIEAANRP
                     DGGAVLSVYLPLEGPSPAAVAAA"
     gene            2368..2982
                     /locus_tag="PLT86_12465"
     CDS             2368..2982
                     /locus_tag="PLT86_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOT37466.1"
                     /translation="MVRRFIPRDSYARKASEQGYLARSAFKLERINEKFHILRPAFRV
                     LDLGAAPGSWLQVASREVGPRGLVVGVDLTPVSFSAPNVVPLTEDILSPDFPDVILPY
                     APFDTVLSDAAPRTTGIRDRDQAGSLALVEAAAGTARSVLKRGGALVAKAFESEDIHR
                     LVRALGNDYASVRTFKPPASRDRSYETYIIAQGKKEQAALEKSG"
     gene            complement(2979..3620)
                     /locus_tag="PLT86_12470"
     CDS             complement(2979..3620)
                     /locus_tag="PLT86_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37467.1"
                     /translation="MPPESSSRSVHIPVQKRGSTFEFLYGGSLPEIRDGTKGYLIVPL
                     DSLSPEDRAAFEKLHTEPLLPRDTRLLVLVQATVFADELLIPSSEAFAIPVDGAFVEV
                     ELQEDLTILLRGTKPPILSSCPCYIPRLDIQARSLNHAYSLISERYEPHRASHTGNVF
                     QKVFHRSADGTWEPLEALRKAAQKSEEERLGEHRRKVAGTTSWFARVASRLKT"
     gene            complement(3732..4568)
                     /locus_tag="PLT86_12475"
     CDS             complement(3732..4568)
                     /locus_tag="PLT86_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT37468.1"
                     /translation="MPEEIAGPLHDVTVEIIAIGTELVMGRIQDTNTFWMEQQIVPLG
                     GHVRRALMVEDDREEIIDAVNSAISRRTAFVITTGGLGPTPDDLTSECLADIAGTVPV
                     PHEPTLDEFVRRRNLKGREELTWNLIRMATVPKTATVLPNPIGWAPCVKLQIGNGTGK
                     TTVFAVPGPPNEMQGVFTAHIAPEIAKATGICRLAERVTIDMWESEASGLFQEVMRRF
                     PGSFLKGYIAMRSAPEDRLPVDIIVAHSDPEEARRRLCDAVTLFGELVTAAGRTMERG
                     KS"
     gene            4713..>5126
                     /locus_tag="PLT86_12480"
     CDS             4713..>5126
                     /locus_tag="PLT86_12480"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT37469.1"
                     /translation="MNFWTILIVRGGGRGTGYDSASLPAQGSKPGFLFGFIAPEREED
                     EMVCRMSRCRIWCAVLATTLLAVFSALRAGTTGKITGVVRDERGRPLPGVAVQITGLR
                     LGGVTDADGAYSILQVPPGTHEVQANMVGFRTLISR"
BASE COUNT         1012 a         1494 c         1632 g          988 t
ORIGIN      
        1 gggcggggat cccatcccac gtgcgtgttg ctcgattgca cgaggcagat gaagctcatg
       61 cgcggccccc agacccgcac gggaagtgag atctcgccgg tatcaccggt agtttcgttc
      121 gtaacgcgga gtcgcagcat ccccttttcg atccgcctgc ttgccgcgaa gtgaacgggc
      181 acctccccgg tgcctctggt ggttgcctcg cgcggacgca gaccatccgg caccgccgcc
      241 ggttcgacgc ggatggtatc gggggagcgg agggttaccg tggcaccacg gccgatgatg
      301 ccgacaacag actggggaac gcgaacagca ttcatggggc gaaacccttt gtgcgttctg
      361 gcaagggatg cacgggaatt cagggggtgc gttgaggatt aagaatcggg cactcggtag
      421 tgtgcgtcaa cgggagggag gcaagggaac ggcgcagagg gaggcacccg cacgaaaaag
      481 ggctcccgat cagcgaccgg gagccctcaa aactggttgc cccccaggga ttcgaacccc
      541 gataccatga tccagagtca tgtgtcttgc cgttggacga gggggcagcg atacaaaata
      601 taccgccgcc gccgggttcg gtcaaccgaa ctctgctccg gaccgcggcc tttcgcaatt
      661 tcgccgggtg cgtaacactt gtccgcaaag ttgtagtaat ttaatagcta agcgcgcaag
      721 gcacacgcgc gaacctcgtt gcggggaacc agttgacgga gtgcagcatg gcgaaagtcc
      781 ttgtcatcga cgacagtgag ccgttgctgg attattacgg caaaatcctg aaggacatcg
      841 gctgcgaggt gctcaccaca tcgctgggtg aggagggaat ccggctggcg cgtgaacatg
      901 cgccggattg catcatgctg gacatcatga tgtcggatat ggacggactc gatgtgctgc
      961 aggaactggc ggattttgat tccaccatcc cggttattgt ggtgacgggt atgccgagcg
     1021 cggagagcgc cattgaagcc ctcaaacggg gtgcgttcga cttccttacg aagggatgca
     1081 cggtagaaga aatcatcgcc accgcacggc gcgcgctgga ccggcgaagg ctttacctgg
     1141 agaaccagga actggttgcc aaactgaggg aggccaactc ccgcctggaa cagcagataa
     1201 gtgaagcgac cgagcaggtg cgggaactga acaacttcaa ccagagcctt ctggaaggca
     1261 ttgacgcagg gctgctggcg gcgagcgaat ccggagtgat tctgttcgcg aacgccgctg
     1321 cgcgcaggat gttgtcgctg agtccccacc aggttatcgg atccaatctc cgggattttg
     1381 ggtttgaggt tacccgggcc gctcacacag tgcggcggag cgagacaccc catatcgtga
     1441 acgggcactt tgaagacagc ctcgactccg tggagaagaa gctcgaacgt gtgcagaagc
     1501 gtacgacgta ccgcctgccc gacggatccg tccgcgtgtt cgggtattcc gtttccacta
     1561 cgcgggacga cgacccgacc ggttcgggac ggcgttcgat cgtcctgttc cgcgacatga
     1621 ccgacatgga agagctccgc aaccagatgc aacggttgca gaagctggaa gccctgaacg
     1681 tggtggtagc cggtgtggcg cacgagatca aaaacccgat cgccgggatc aaaagcgtgg
     1741 cgtcgattct ggtggaaacc atgaaggaag acgacccgct ccgagatcac gtcctccgta
     1801 ttctcgaaga atcacgccgc gtaacacgtc ttgtggaaga gtttttctcg ttttcgcgcc
     1861 catcgaaacc gaaactcgaa gccgctgacg tttctgacat cattggccgc gtggtgcggc
     1921 tcacccatga cgccgccacg caacgaaggg ccgatattgc caccgtggtg gcgctcgaca
     1981 tcccgcgcgt gccgatggac aaggatcaga tccagcaggt gatcctgaat ctcgtcctca
     2041 acgcccttga agcgatcggg cagaatggca ggatcgagat ctccgccgat gtggtcgatt
     2101 accatgttct tggccggcga tgcgcgcgcg ttcaggtgcg ggattccggc cccggtttcc
     2161 ccgcggacgt gttccaccgc ctgttcgatc cgttcttcac aacgaaacct tccggaaccg
     2221 ggctgggcct ccatatatcc cagaacatcg tgcttgagca cggcgggcgt atcgaggcgg
     2281 caaatcgccc ggacgggggc gcggtgctga gcgtgtatct tccgctggaa ggcccctccc
     2341 cggccgctgt tgcggccgcc tgaccgcatg gtaagacgtt tcataccccg cgactcgtac
     2401 gcccggaagg cttcagaaca gggttacctt gcacgcagcg ccttcaagct ggaacgtatc
     2461 aacgagaagt tccacatcct gcgtcctgcc ttccgcgttc tggatctcgg cgcggcgccg
     2521 ggaagctggc ttcaggtcgc cagccgcgaa gtgggaccgc gggggctggt ggtgggtgtt
     2581 gatctgaccc cggtctcatt ctccgcaccg aacgtagtac ccctgacgga agacattctg
     2641 agtccggatt tccctgacgt cattctcccg tatgcgccgt tcgacacggt tctgagcgat
     2701 gcagcccccc gcacgacggg gattcgtgat cgggaccagg ccggctcgct ggcgcttgtg
     2761 gaggcggcgg cgggtactgc tcgttccgtt ctgaaacgcg ggggcgcgct cgtggcaaag
     2821 gcattcgaga gcgaggacat tcaccggctg gtgcgcgcgc ttgggaatga ctatgcgagc
     2881 gttcgtacgt tcaagccacc cgcctcgcga gaccggagct acgaaaccta catcatcgcg
     2941 caggggaaaa aggaacaggc ggctctggaa aaaagcggtt aggttttgag ccgactggca
     3001 acgcgcgcga accagcttgt ggtcccggca actttcctgc ggtgttcccc gagtctttcc
     3061 tcttcgcttt tctgggcagc cttccgcagc gcttctagcg gctcccaggt tccgtccgca
     3121 gaacggtgaa acactttctg aaacacgttg ccggtatgcg atgcgcggtg cggctcgtaa
     3181 cgctcggaaa tcagcgaata ggcgtggttg aggctgcgag cctggatatc cagacggggg
     3241 atgtagcacg gacaggaaga gagaatcggg ggtttggttc cgcggaggag gattgtgagg
     3301 tcctcctgca gttcaacctc cacaaacgcc ccgtccacag ggatggcaaa tgcctcggag
     3361 gagggtatca acaactcgtc ggcaaacacc gtcgcctgca ccagaaccag aagacgggtg
     3421 tccctgggga gcagaggctc ggtgtgaagc ttctcgaacg cggcgcgatc ctcggggctg
     3481 agtgaatcca gcggcacaat gaggtacccc ttcgtgccgt cgcgaatttc cggcaggctg
     3541 ccgccgtaca gaaactcgaa ggtggaaccc cttttctgca cggggatgtg gacacttctg
     3601 gatgacgact ctggaggcat acgcaccacc ctttcgcgca cccgaagctc accctgaaat
     3661 tgccgctcct ttaagttcat gcacgcgcgg cgaagatgca aatcctgacc gcggaacaag
     3721 gggaacgggc actacgattt cccccgttcc atcgtccggc ccgccgccgt aaccagctcc
     3781 ccgaaaagcg tgacggcgtc gcagagcctg cgccgggcct cttccggatc actgtgagcg
     3841 acaatgatat ccacggggag gcgatcctca ggagcggacc tcatggcgat ataccccttg
     3901 agaaacgaac cggggaagcg ccgcatgact tcctgaaaca accccgaagc ttccgattcc
     3961 cacatgtcaa tcgtcactcg ttcggcaagg cggcagatcc ccgtcgcttt tgcgatctcc
     4021 ggcgcgatgt gcgcggtaaa cacgccctgc atttcgttgg ggggaccggg aaccgcgaac
     4081 actgttgttt tgccggtgcc attgccgatt tgcagcttca cacagggtgc ccacccgatc
     4141 ggatttggca gaacggtggc ggttttggga accgtcgcca tgcgtatcag gttccaggtg
     4201 agttcctcgc ggcctttcag gttccttcgg cgcacgaatt cgtccagcgt tggctcgtgg
     4261 ggaacaggta cggttcccgc aatgtcggca aggcattcgg aggtgaggtc gtcgggggtg
     4321 ggccccagcc ctcccgtggt gatgacgaag gccgtgcgcc gggagattgc gctgttcacc
     4381 gcatcaatga tctcttcccg gtcgtcctcc accatcaggg cgcggcggac gtgccccccg
     4441 agtggaacta tctgctgttc catccagaag gtattggtat cctgaatccg ccccatgacg
     4501 agttccgtgc cgattgcgat gatctcgacg gtgacgtcgt gcaggggccc tgcgatttcc
     4561 tcaggcatgt gttccacggt tgtgaaggca gaaaaccaga acggtgacgt gaatgcagat
     4621 gtgacatgtg aaatgatacc ggtgcgcgaa acggagggac aagagcgtgt tccgacacgt
     4681 ccctgggcgg attgcccctg acgacctgcg gagtgaactt ttggacgatc ctgattgtcc
     4741 gtggcggggg ccggggaacc ggttatgatt cggcgagtct gccggcgcaa ggctccaagc
     4801 cgggattcct gtttggattc atagcgcctg agcgtgagga ggacgagatg gtctgccgca
     4861 tgtctcgttg tcgtatctgg tgtgcagtac tggcaaccac ccttttggcc gttttctccg
     4921 cactacgtgc gggaacaacg ggcaaaatca cgggggtggt gcgagacgaa cggggaaggc
     4981 cgcttccggg ggttgcggtc cagatcacgg gcctccgact tggcggggtg acggacgccg
     5041 acggggcgta ttccatcctt caggtgcccc ccggaacgca cgaagtccag gcgaacatgg
     5101 tggggttccg cacgctcatt tcgcgc
//
LOCUS       DAOSOO010000218         5125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16414_length_5125_cov_6.212229, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000218 DAOSOO010000000
VERSION     DAOSOO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16414_length_5125_cov_6.212229"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLT86_12485"
     CDS             <1..380
                     /locus_tag="PLT86_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37470.1"
                     /translation="LTFVRKPATRSEVIDAVRRMKTAGLNVGVIAMVGVGGDRFASTH
                     VEETINAINAMELGDGDIVYLSDFVNHRGLPYERLARERGIRDLTSLELKTQRNGIRA
                     GLRWKDPGKPAQVSLYDIREFIY"
     gene            complement(383..1132)
                     /locus_tag="PLT86_12490"
     CDS             complement(383..1132)
                     /locus_tag="PLT86_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT37471.1"
                     /translation="MRLTRSLYVVGGHCVGLSDTQDCIIYVVAAPEGLIMVDAGGGRD
                     PEAIFRAMAEEGLDERQLRVLLLTHCHADHAAGAAAIRERTGCAVAISERSAHLLENG
                     TEIDVRLDLAKAMRIHPPDLVWANCPVDIRLSDGQLLDILGITVHVFEVAGHSEDSCC
                     FVMEVDGRKCLFAGDVIQYGGVIGLLNYPGSTLDGYRRDLSKLRGLGIEALLPAHSLF
                     TMRNGQHHIDSAIERLKSHIVPPTVGQKVFF"
     gene            1364..3616
                     /locus_tag="PLT86_12495"
     CDS             1364..3616
                     /locus_tag="PLT86_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT37472.1"
                     /translation="MSFLDQLNPEQRRAAQILSGPVLILAGAGSGKTRTLTHRLAHLI
                     ASGTPAHRILAVTFTNKAAKEMKERTARLIGEARAASLWIGTFHSICARILRRDAHLL
                     GYDARFTIYDSDDSLTLVKRIMRDHGTLDDSLKPGIVRARISEAKAFLLTPEEYARNR
                     KGPAAAVIADLYRRYQAALISNNAMDFDDLLALPVRLFRTQPEVLEEYRNRFQHLLID
                     EYQDTNQAQYVLVKMLAESHHNICAVGDDDQSIYGWRGADITNILNFENDFPDATVVR
                     LEQNYRSTRTILKAAGAVVRNNRTRKVKELWTQNDPGDHLTIAELADEKDEAEWIRER
                     LVRLLEDGHSYGDIAVLYRTNAQSRAIEEAFVRSSKPIPYTVVGGLRFYERKEIKDVL
                     AYLRVIDNPRDSESLRRIVNVPRRGIGEKTMALAAERMGATGLPLLDALRTLEEHELG
                     PRAYKPINEFIRWLDALIANKGEMAVDELLEKILTDTGYRAQLEQDGSVEAETRLQNI
                     GELVAGATAYVEANNEPNVSAFLQDIALVTDADEVQTDGGSSVTLMTLHSAKGLEFPV
                     VFVSGMEEGLFPIAGSLQDSAELEEERRLFYVGLTRAQKLVFLSYAQRRRRFNEWANT
                     QPSRFLREIPDELLKWDTRSTEEPGRRILDQRRFREDGSVGVRGISTRRIHAVGDEFS
                     QESSYADDFSQTTDSFLAIGHHVMHPMFGRGRITAREGLGLDTKLTIHFDSGQTKKIL
                     VRVAHLEPCV"
     gene            complement(3678..4928)
                     /locus_tag="PLT86_12500"
     CDS             complement(3678..4928)
                     /locus_tag="PLT86_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT37473.1"
                     /translation="MNGKPVLTIARREYIARVKTKSFVVMTVLIPLIMGASVAAPMFL
                     VKAESPRQRVLAVADETGLVFDSLRSLREETLPNGKPRYLIERVAATPRAVSDARRLV
                     ELEAYDAFAFVPAGAVDGLPVTYYTNGVPNPQEVDHVRGAFNRVLIAGRLAREGLDPA
                     RVSALTRSVRFDVVALQKGAAPKTAQAAFAAAVALAILMYISLTMYGAFTMQSVVNDK
                     ASRVVEILVSTVSPTELMYGKILGTGCAGLTQLGIWMAVILGVSIGMPESAVAKTFHA
                     AADVSIPALILFFVTGFFLYASLYAGVGAICTTNEEAGQLQLPVSLLLMVAFFLAVSG
                     VNDPGSTLITVLSYVPFFAPVLMMLRFGVGEASWFDAAVSLAGVVLTTAIIAAAVGKI
                     FRVGILMTGKRASLTEVLRWLRRA"
     gene            complement(4925..>5125)
                     /locus_tag="PLT86_12505"
     CDS             complement(4925..>5125)
                     /locus_tag="PLT86_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HOT37474.1"
                     /translation="DRVPDFLEPDDMIAAVEIVSDGARIRLREGADAQMVLDRAIRRG
                     RVLKFEIVEPSLDDLFIEAVSR"
BASE COUNT         1175 a         1551 c         1465 g          934 t
ORIGIN      
        1 tgctgacgtt cgttcgcaaa cccgcaacgc gctcagaagt catagacgcg gtccgccgca
       61 tgaaaacagc cggattgaac gtgggagtaa ttgcgatggt cggggtgggt ggagacagat
      121 tcgcttccac gcacgtggag gagactatca acgcaatcaa cgcgatggaa ctcggcgatg
      181 gcgacatcgt ctatctgagt gacttcgtga accaccgcgg actgccttac gagcggctgg
      241 cgcgggaacg cggaatcaga gacctcacca gcctggaact gaagacgcaa cggaacggga
      301 ttcgggcagg cctacgctgg aaggatccgg gcaaacccgc tcaagtctct ctttacgaca
      361 ttcgtgagtt catctactga tttcagaaga agaccttctg cccgacagtc ggaggaacga
      421 tgtgggattt cagccgctca attgctgaat caatgtggtg ttggccgttc cgcatcgtga
      481 acagcgaatg tgccggcaac agcgcctcaa tcccgagtcc gcgcaacttc gaaagatccc
      541 tgcggtatcc gtcgagggtg ctacccgggt agttgagcag cccgatcacc ccgccgtact
      601 gaatcacgtc cccggcgaac aggcatttcc ggccatccac ttccatcacg aagcaacacg
      661 aatcctcgct gtggccagcg acttcgaaca catgaacagt gatgccgagg atgtccagca
      721 gctgcccgtc ggaaaggcga atgtccaccg ggcagttcgc ccacacgagg tccggcggat
      781 ggatgcgcat ggctttggca aggtccagac gcacatcgat ctccgtcccg ttttccagca
      841 ggtgggcaga tctctccgaa attgccaccg cacatccggt acgttccctg atcgccgcag
      901 ctcccgcagc gtggtcggcg tggcagtgcg tcagaaggag aacgcggagt tggcgctcat
      961 ccagcccctc ctccgccatg gcgcgaaaaa tcgcttccgg atcgcgcccg ccgcccgcat
     1021 ccaccattat caggccctcg ggagccgcca caacgtatat gatgcaatcc tgcgtatcgg
     1081 agagacctac acagtgaccg ccaacaacgt aaagagagcg ggtcaatctc atggaacaca
     1141 cccccaaatt cggcgttcgt cgacgttacg cagcctgtga aagtaatatc ccgttcagtt
     1201 gcggcgacgc caacacctgc gtgcagaacg gtgggcccgc gcggcaagac agaaacgccc
     1261 gcggccttcg tatctttgga aacgaaatga ttcgcccgga tcgctcgatc ctgccggaca
     1321 gtgcacccac gtacgatctc gaaaacctga ggaccccagt ttcatgtcgt ttctcgacca
     1381 gctcaatcca gagcaaagaa gggccgcaca gattctttca ggccccgtcc tgattctcgc
     1441 cggggcgggc agcgggaaaa cccggaccct cacacaccgg cttgcacacc tgatagccag
     1501 cggcacccct gcacacagga ttctcgcggt aacattcacg aacaaggccg cgaaagaaat
     1561 gaaagagcgt actgcccggt tgatcggtga ggcccgagcc gcgtcgctgt ggatcggcac
     1621 gttccactcc atctgtgcca gaatactgcg tcgtgacgca catctactcg gctatgacgc
     1681 gagattcacg atttatgaca gcgatgattc gttgacactt gtgaagcgca tcatgcggga
     1741 tcacgggacg ctggatgaca gcctcaaacc ggggatagtg cgtgcgcgaa tatcggaggc
     1801 aaaggccttt cttctcacac ccgaagagta cgcccggaac cggaaaggac ccgccgccgc
     1861 agtgatcgcc gatctctacc gacgttatca ggccgcgctg atttccaaca acgcgatgga
     1921 tttcgatgac ctgctggcgc ttccggttcg tcttttccgc acacaacctg aagtactcga
     1981 agaataccga aaccggttcc aacatcttct cattgacgaa tatcaggaca cgaaccaggc
     2041 tcagtacgtt cttgtcaaaa tgcttgccga gagccaccac aacatctgcg cagtaggcga
     2101 tgatgaccag tcgatttacg gatggcgagg cgccgacatc acgaacatcc tcaacttcga
     2161 aaacgacttt cccgacgcca cggttgtgag attggagcag aattaccgtt ccacgcggac
     2221 gattctcaaa gccgcggggg cagtagtgcg caacaaccga acgcggaagg tgaaggagct
     2281 ctggacacag aacgatccag gcgatcacct gacaatcgcc gagcttgctg acgagaagga
     2341 tgaggcggag tggattcgcg agcgtctggt tcgacttctg gaggatggcc actcttacgg
     2401 agacattgcg gtcctctatc gcacaaacgc ccaatcacga gcgattgagg aggcgtttgt
     2461 tcgatcttcc aaacccatcc cgtacacggt ggtcggtggt ctccgcttct acgaacgaaa
     2521 ggaaatcaag gacgttctcg cgtatctgcg ggtgatcgac aatccacgtg actctgagag
     2581 cctcaggcgc atcgtgaacg tcccgcggcg cggcatcggc gagaaaacga tggcgttggc
     2641 cgcagaacgg atgggagcaa cgggtctgcc cctcctagac gcgctgcgaa ccctggagga
     2701 gcacgaactc ggcccccgcg cgtacaaacc gatcaacgag ttcatacgat ggctcgacgc
     2761 actgatcgcg aacaaaggcg aaatggcagt agatgagctt cttgagaaga ttctgacgga
     2821 cacgggatac cgtgcgcaac tggaacaaga cggatccgtt gaggccgaaa ctcgcctcca
     2881 gaacatcggg gaactggttg caggcgctac cgcgtacgtg gaagcgaaca acgaacccaa
     2941 tgtcagcgcg ttcctccagg atatcgcgct ggtgaccgac gcagacgagg tgcagacgga
     3001 tggaggcagt tcagttaccc tgatgacgct ccattccgcc aagggtctcg aattcccggt
     3061 agtatttgtg tcaggcatgg aggaggggct cttcccgatc gcaggctctt tgcaggactc
     3121 agcggaattg gaggaggagc ggcgtctgtt ctatgttggc ctcactcgcg ctcagaagct
     3181 ggtgttcctg agctacgcgc aacgccgccg gcgtttcaac gagtgggcaa acactcagcc
     3241 ttcgcgattc ctgcgggaaa tcccggatga attgttgaag tgggacacga gaagcaccga
     3301 ggaacccgga aggcgtatcc tggaccagcg gcggttccga gaagacggat ctgtcggcgt
     3361 gcgagggatc tcaactcgtc gcatccacgc ggttggggat gaattttctc aggaatcgag
     3421 ttatgccgac gacttctccc agacaacgga cagtttcctc gccatcggac accatgtgat
     3481 gcacccgatg ttcgggcgtg ggagaataac ggcccgcgaa ggcctcggac tggataccaa
     3541 gctgacgatc cacttcgaca gcggacagac gaagaagatc ctagttcgtg tcgcccatct
     3601 ggagccttgc gtctaggact gagtggggta cctgacgcga cgcttgctgc cctcggcatc
     3661 ctctgaccgg aaatcagtca cgctctccgc agccagcgga gcacctccgt cagcgacgct
     3721 cgtttccccg tcatgagtat gcccacccgg aaaatcttcc ccacggcggc cgcgataatg
     3781 gccgttgtga ggacaacccc cgccagtgag acggcggcgt cgaaccaact tgcctctcca
     3841 acgccgaaac gcaacatcat gagaacagga gcgaaaaacg gcacgtacga cagcaccgtg
     3901 atcaaggtgc taccggggtc gttcacgccg gacaccgcca ggaagaacgc aaccatcaga
     3961 agaagcgaca cgggcaactg gagttgcccc gcctcttcgt tcgtcgtgca gatcgcaccg
     4021 actccggcgt agagcgaagc atacaggaaa aagccggtca cgaaaaaaag gatgagggcg
     4081 gggatcgaga catccgccgc ggcatggaac gtcttcgcaa ccgcactttc gggcatgccg
     4141 attgagacac ccagaatcac tgccatccag atgcccagtt gtgtgagtcc ggcacaaccg
     4201 gtgccaagga tcttcccata catcaactcc gtgggcgata ccgttgacac gaggatctcg
     4261 acaacccgcg acgctttatc gttcacaaca ctctgcatcg tgaacgcgcc gtacatcgtc
     4321 aagctgatgt acatcaggat cgccaatgcc acagcggccg cgaaagctgc ctgggcggtt
     4381 tttggcgcgg ctcctttctg caacgcaaca acgtcgaacc ggacggaccg tgtcagggcg
     4441 gagacacgtg ccggatcaag cccctcccgc gcaagcctgc cggcaatcag taccctgttg
     4501 aaggcacccc tgacatggtc tacttcctgg gggttcggca caccgttggt gtaatatgtt
     4561 accgggagac cgtcgacggc ccctgccggt acgaaggcaa acgcgtcgta ggcttccagc
     4621 tccaccagcc tgcgcgcatc agacactgca cgaggagtcg ccgccacccg ttcgattaga
     4681 taacgcggtt tgccgttcgg cagggtttcc tcccgcagcg accggagtga gtcgaatacg
     4741 agcccggttt cgtccgcaac cgcgagcacc cgctgtcggg gagattccgc tttcacaagg
     4801 aacatcgggg ccgctacgct tgcacccatg atcaggggga tgagaaccgt catgacgaca
     4861 aaacttttcg ttttgacgcg tgcgatgtac tcacgccgcg cgatggtgag cacgggtttt
     4921 ccgttcatcg gctcaccgcc tcgatgaaga ggtcatccag ggagggctcc actatctcga
     4981 acttcagcac gcgccctcgc cgaatcgcgc ggtcaagcac catctgcgca tccgcaccct
     5041 cccgaagacg gatccgcgcg ccatcggaga ctatttcgac ggcagcgatc atatcatcgg
     5101 gttccagaaa gtcgggaaca cggtc
//
LOCUS       DAOSOO010000219         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16532_length_5097_cov_5.471440, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000219 DAOSOO010000000
VERSION     DAOSOO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16532_length_5097_cov_5.471440"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLT86_12510"
     CDS             <1..321
                     /locus_tag="PLT86_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOT37475.1"
                     /translation="QKRGASVIAARGASSAASAANAIIDHVKSFLLPTPGGHWFSAAV
                     KSDGSYGVPAGLVSSFPITKKETDNWCIVPDLILSEYGKRMLANTVRELESERDMVKD
                     MLGS"
     gene            369..1385
                     /locus_tag="PLT86_12515"
     CDS             369..1385
                     /locus_tag="PLT86_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT37476.1"
                     /translation="MAILLMKDWVEERLKTPPRNSCAATTPAEFLEWQRSFRRSIKRA
                     LGQFPKSVPLAPKTLEKTDCGTYTREKVVFHSEPAMAVVAWVLAPKRTKRGQRVPGLV
                     CAHGHGLGKNPLVGLDNNGQVHEDYQHRHAIQFAERGYVTIAPDWRGFGERRDGPPWD
                     DKGRDGCNLRHLAAGYFGFSLLALQIHDGMRCIDYLQSRKEVDPSRIGCVGVSFGGTM
                     TTYLAAMDRRVKAAVISCYLSTIVNGLRRTNFCGAQYMPELRAHGDIPDVALLAAPRP
                     LLAEVGEKDRCFEVDDALQACERVRAGYLAAGHPDRFDVDLFPGEHEFSGRKAFDWFD
                     RWLK"
     gene            complement(1393..2523)
                     /locus_tag="PLT86_12520"
     CDS             complement(1393..2523)
                     /locus_tag="PLT86_12520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT37477.1"
                     /translation="MLAISIIAFVLALGYVVSAGILAGALRGNRRVRVPASAANTSVT
                     VVIAARDEEEALSVCLESLSRQDYPPDAWNLVVVDDRSQDGTGQVVRRARDENDSIHA
                     LRVDECDPAYGGKQNALAAGIEHMAGMGILGDIILFTDADCVVPSSWIADMVAAFSSP
                     RVGMVAGWTTIPTTRSISSLLEKCELAYLLSVAFGMIALGRPVSAIGNNLGIRRKTYE
                     EVGGYRNLGYTITEDCALVQRVAATSWSITTARDAAAVLTRPCSSIRDFVRQRIRWAA
                     GVRHLQVGQMLLPAFAFAHRSWIALTSAMAWCGVLDWRIPAMVWCSSFLADAALIGAR
                     KNWKWAPMSPLLTLWQVIYQPVVAIMVLLSGRTRWRGSRYRL"
     gene            complement(2582..3589)
                     /locus_tag="PLT86_12525"
     CDS             complement(2582..3589)
                     /locus_tag="PLT86_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37478.1"
                     /translation="MPVSSMKSLPLRTYLRAASAIGENIRKKEAINPLYASYKVTNRC
                     NFRCKFCNVWFEKTPVLETELVYRVLDNLERAGVFLLSLEGGEPLLRNDIGDILKYAG
                     AKDYYILFTTSDRSLTEQPMVEYCRNIDFLHISIDEGHDNLDMFSLLPEAVRWGSIVC
                     VQVVVMKENLPALEDKVARCRDAGAKCVIMPAVELNRTRNHFPDLDAFQRECLRLKRK
                     YPRTIISPDPYFAALREPHGCSTGSIIVDCDGGLFYPCRTLESKPVNLLDVDLRDWLH
                     GEEAKAFRRQMATCDRRCGWYQYYAVSAFTSPVHVWTSISPYFWNVLAPRKKQNHNGR
                     K"
     gene            3981..>5097
                     /locus_tag="PLT86_12530"
     CDS             3981..>5097
                     /locus_tag="PLT86_12530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT37479.1"
                     /translation="MSERGAVLIVGDDQATCETLSDFLTSAHHTVHVVTSAPEAITEL
                     QNRPYDIVVLDALMAGETGLDLVARIKAVDFRVQVIALMDIGAGEAAVRAISQGASSY
                     LLRPVRLAALLNRVATALHTRSFFRSLQEPVSDPERRPDLVRGLEIAEKLFQFDRELV
                     SILDYHLVVESVLRSALSIAEADMTAFLMIRDRVVSIVVNVKEDSPLASRQAVFERMT
                     SKWDSWGGNSLHLDDVLWSGLEEDALGSTREASVYPIVVQDVLVGAIGAFATGRRSLD
                     PRADVLVPIIASRAEVVIENAFLHEHTKILATTDPLTGLLNRRVFREGIVREFERSRR
                     LFLNKRPGGDLSVIMLDVDHFKKFNDTYGHQLGDKVLK"
BASE COUNT         1104 a         1492 c         1460 g         1041 t
ORIGIN      
        1 cagaagcgag gggcaagcgt gattgcggct cggggggctt cgtctgccgc gtctgcagcc
       61 aatgccatca tcgaccacgt gaagtcgttc ctcctcccga ctccgggggg acattggttc
      121 agtgctgccg tcaagtccga tggtagctac ggcgtgccgg cgggactcgt atcgtcgttc
      181 ccgatcacaa aaaaagaaac ggacaattgg tgcattgtac cggacctgat tctcagcgaa
      241 tatggcaaga gaatgctcgc gaacacggtc cgcgaactgg aatcggaacg cgatatggtg
      301 aaggacatgc tgggttcgtg aatgcccggc ggtgatgttc cacagtcacg caaagaaagg
      361 gaactactgt ggcgatactc ctgatgaagg actgggtcga agagcgtctc aaaactcccc
      421 cgcggaattc ctgcgcggca acgacacccg ccgagttcct cgagtggcag agatccttcc
      481 gacgttcaat caaacgtgcg ttggggcagt tccccaaaag tgtaccgttg gctccaaaaa
      541 cgctggagaa gacggactgt ggcacgtaca ctcgcgagaa ggtcgtgttt cacagcgagc
      601 cggcaatggc ggtggtggcg tgggtccttg cacccaaacg cacaaaaagg gggcaacgtg
      661 tgcccggtct tgtatgcgcc cacgggcacg gacttggcaa aaacccactc gtaggcctcg
      721 ataacaacgg gcaggttcac gaggattacc agcaccggca cgccattcag tttgcagagc
      781 gcggctacgt aaccatcgcg cctgattggc gtggatttgg cgagcgaagg gacggacccc
      841 cgtgggacga taagggccgt gacggctgca acctccgtca cctcgccgcc ggttatttcg
      901 ggttttcact tcttgcgctg cagatccacg acgggatgag gtgtattgat tacctgcaat
      961 cgcgaaaaga ggtggacccg agccgaatcg ggtgcgtcgg cgtatcattc ggcgggacca
     1021 tgacgacgta tctggcggcc atggaccgga gagtaaaggc ggcagtcatc tcgtgctacc
     1081 tgagtacaat cgtgaatggc ctcagacgca cgaatttctg cggagcgcag tacatgccgg
     1141 aattgcgcgc acacggcgac ataccggatg ttgcgctgct tgcggcccca cggcctttgc
     1201 tcgctgaggt aggtgaaaaa gaccgctgtt ttgaagtgga tgacgcgctc caggcatgtg
     1261 aacgtgtcag ggctggttac ctcgccgccg gccaccccga tcgtttcgac gtggatctgt
     1321 ttcctggaga gcacgagttc agcgggcgca aagcgttcga ctggtttgat cgctggctga
     1381 agtgagatga gatcataagc ggtatctgga tccgcgccag cgcgttctcc ctgatagcag
     1441 gaccatgatg gcaacgacag gctgatagat cacctgccac agagtcagaa gcgggctcat
     1501 cggtgcccat ttccagttct ttcgggcacc gatgagggct gcgtccgcga ggaaactcga
     1561 gcaccacacc atagcaggga tgcgccagtc gagcactcca caccatgcca tcgccgaagt
     1621 gagggcaatc cacgaccggt gagcaaaggc aaacgcaggc agaagcattt gcccgacctg
     1681 gaggtgacgt acgccggccg cccagcgaat tctctgccgc acgaaatctc tgattgacga
     1741 gcacgggcga gtaagcacgg cggccgcatc ccgggcggta gttatgctcc atgatgtcgc
     1801 agcgactcgt tgaacgagtg cacaatcctc agtgatcgtg tagccgagat tccggtatcc
     1861 ccccacctcc tcgtacgtct tgcgccttat ccccagattg ttgcctatcg ccgatacggg
     1921 acgcccgagc gcgatcattc cgaatgcaac gctgaggaga tacgcaagtt cgcacttttc
     1981 cagcaaggac gagatcgacc gggtcgtggg aatcgtcgtc caacctgcca ccatgcccac
     2041 gcgggggctg gaaaaggctg ccaccatgtc ggcgatccac gaagaaggca ctacacaatc
     2101 cgcatccgtg aacaggatga tgtctccgag tatgcccatt cccgccatgt gctcgattcc
     2161 agccgcaagt gcgttctgtt tccctccgta tgcaggatcg cactcatcca cgcgaagcgc
     2221 atggatactg tcgttttcat ctcgagcccg acgaaccacc tggccagtgc cgtcctgcga
     2281 ccgatcatca acgacgacca gattccacgc gtccggcggg taatcctgcc tgctgagact
     2341 ttccaggcat acgctcagcg cctcctcttc gtcacgtgca gcaatcacta cggtcaccga
     2401 cgtattcgcc gcggaagcgg gcaccctcac ccgtcggttc ccgcgcaggg ccccggcgag
     2461 aatccctgcg cttaccacgt agcccagagc gagaacgaag gcgatgatac tgattgccag
     2521 cacgtgacct caaatccagg gagagagacg gagcccgggc tgaccccttt gacccgacac
     2581 ttcactttct cccattgtga ttctgtttct tgcgcggcgc cagtacgttc cagaaatacg
     2641 gggaaatgga cgtccacacg tggacgggcg aagtgaacgc gctcaccgcg tagtactggt
     2701 accagccgca ccgccggtcg cacgtggcca tctgccggcg aaacgccttc gcctcttcgc
     2761 cgtgcagcca gtcccgaagg tcgacatcga ggaggttgac cggcttcgat tccaacgttc
     2821 tgcacggata gaacagccct ccgtcgcagt cgacgattat cgatccggtt gaacacccgt
     2881 gtggctcacg aagggcggcg aaatacgggt ccggcgatat tatcgtccgg gggtactttc
     2941 gtttgagcct gagacactcg cgctgaaacg catccaggtc agggaagtgg tttcgggttc
     3001 ggttcaactc cacggccggc ataatgacac atttggctcc ggcgtctctg catctcgcaa
     3061 ctttgtcctc caatgccggc aggttctctt tcatcacaac cacctgcacg cacacaatgc
     3121 tgccccaacg gaccgcttcc ggcagcagtg agaacatgtc cagattatca tgaccttcat
     3181 cgatgctgat gtggaggaaa tcaatgttcc ggcagtattc gaccatcggc tgctcggtga
     3241 gggaacggtc gctggtggtg aagaggatgt agtaatcctt tgctcccgcg tacttgagga
     3301 tatcgccgat gtcgtttctc agaaggggtt caccaccctc gagtgaaagc agaaatactc
     3361 cggcacgttc caggttatca agtactcggt acaccagttc cgtttcgagt accggcgttt
     3421 tctcaaacca cacattgcag aacttgcacc tgaaattgca gcgattcgtc accttgtatg
     3481 aagcgtacag agggttgatt gcttcctttt tccgaatgtt ttcgccaatt gccgaggcag
     3541 cgcggaggta cgtcctcaac ggcaacgatt tcatggaaga aactggcatg gcagaccacc
     3601 tcaaaacaga accagcgacc gtatgatcgc gcgtctaaca acgtactcgc cgcggtttcc
     3661 ccgtgcaagc ttagcctgct ccccgatttc ccaccagtga caaaacgatc tgccgatcag
     3721 gcccggaaaa cgacacgctg gccgatttcg cggcatggct ccagagagct tcttgattat
     3781 ctctaacaaa cacattactt tcagtttagc ccacggcatc gcatagtact ctctgctgtc
     3841 cgccagatga cgaagccatg gggtgccccc gcatcaggga tacgccgtcc acagagcaaa
     3901 ccgtgaggct gtcggccgca ccgcgcaccg gcagacggga ttgaatcaga attgcccgaa
     3961 gggcgtttat ggagccgtcc atgtcagagc gaggcgccgt cctgattgtg ggagacgacc
     4021 aggcgacatg cgagaccctc tctgatttcc tcacatcggc gcaccacacc gttcacgtcg
     4081 ttacttcggc tcctgaagca attaccgaac tccagaaccg gccctatgac atcgtggtgc
     4141 tggatgctct catggcaggc gaaacgggtc tggacctcgt cgccaggatc aaagcagttg
     4201 acttccgagt tcaggtcatt gccctgatgg atatcggcgc gggtgaagct gcggtacgag
     4261 ccatatcgca gggcgcaagt tcgtatctcc ttcgaccggt cagactggct gcgttgctca
     4321 atcgcgttgc aacggcgctt catacacggt ctttttttcg gagccttcag gaacccgttt
     4381 ccgatccgga gcgacggcca gatcttgtgc gcggacttga gattgcggaa aaactgtttc
     4441 aattcgatcg ggaacttgtc tcaatcctgg attaccatct cgtcgtcgag tccgtcctga
     4501 gaagcgcgct ctccatcgcc gaagccgaca tgacagcgtt cctcatgata cgtgacagag
     4561 tggtttccat cgtggtgaac gtcaaggagg actctccgct cgcaagtcga caggcggtgt
     4621 tcgaacgcat gacctcgaaa tgggactcat ggggcggaaa ttcgctgcac ttggacgatg
     4681 ttctctggtc cggactcgag gaagacgccc tcggctcgac acgggaagcg agcgtgtacc
     4741 cgatcgtggt ccaggacgta ctggtcgggg caatcggtgc gtttgcaaca gggcgccgat
     4801 ctttggatcc gcgtgccgat gttctggttc ccattatcgc gagccgcgca gaggtagtga
     4861 tcgagaacgc atttctgcac gaacacacga agatccttgc aacaacggat cctctgacgg
     4921 gcctgctgaa ccgacgcgtg ttccgcgaag gaatcgttcg cgagttcgag cggagccgac
     4981 gcctgttcct gaacaagaga cccggtggcg atttgtcggt tatcatgctc gatgtggatc
     5041 acttcaagaa attcaacgac acgtacgggc atcagctggg cgacaaagtc ctcaaaa
//
LOCUS       DAOSOO010000220         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16945_length_4996_cov_5.789719, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000220 DAOSOO010000000
VERSION     DAOSOO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16945_length_4996_cov_5.789719"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4308
                     /locus_tag="PLT86_12535"
     CDS             <1..4308
                     /locus_tag="PLT86_12535"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOT37480.1"
                     /translation="SSVRVRFVTDDNIDAGNTSYNRQSGGWWLDEIRVEELPAPVTLN
                     PITSSSQHHAALSWTQNADTDFSRYEIRRATSSAVTISSDLVKTITTQSTTAWTDTFA
                     LVEPARFSYRVYVVNTLENVSPGSNILTADYTIPLKTFPFTDDMSAATENWSWGDPWG
                     PTSESFHSGPASWKSGPGRAYPPNANTALATRVNLSGATAPVLTFWHRYAFEQAVDFG
                     YVEISSDGGQTWAAVMAVTGVDTMWASERIDLGAYMGSTIGLRFRVTSNATTHLDGWY
                     IDDVTIANGPRMATYPLFDDMESGPNAWFPQSPWGLSNVGSRSGEWHWTDSPAGSYAP
                     NTNSALTLRINLSGAAAPELRFWQRYSFEPNADWGYVEVSGNNGSSWTQACFVTGSQA
                     SWREERVDLSPWKGNADVLLRFRLTSNASNESDGWHIDDISMVEVAREFPYPFVETFD
                     DTAALSRWHTASWEPVTGGRTTPFQMHDSPSGNYPANAFSALTLAGTIDLRGATNPLL
                     TFWHKYAFFSDGSYGHNEYDYGRVYVSPDNGAVWYLVSSYNTSATSWTKASLNLNQFV
                     GASAVRVRFVMDDDVDAANSSYNRQADGWWIDDVRIGENLAVQALADSVRLEGPALAF
                     AVPGLASPRYIGRIYEPSVTSTAGQGANVVGQFGVGPRGTMPNDTSWTWFAAGYLDDV
                     SSFDRFFGSVRVDSSGEFDVAFRGSIDGGATWVYADLDGNNLAGGGVNEYTTAQAARL
                     IVGRGASVRLDVATVERGIRTGTQEFWPFTFSNGGPDVLAWWLAEADSNQAVKDIPWL
                     VIGKSSGTLAEGGRAVVDLTFSAVGLNPGLYKANLMMVSNDALNDTIAIPVRLTVYAT
                     GVTAFTGRVAWTGGGNVAGGTIVASQSNLPVDTIAVSENGQFAAYGIPAGQYDLAVQV
                     PGAYPRAFRNFTLPAENALLTVTPIHPRMVSTSFADFYSPASTLDGQPLAVGSVVSVR
                     NAAGTVCGSCVATEPGKYGLLHVYADDPSTPETEGASIGDTLAFFVNDVPTGAKAVYT
                     AHFSVTRVDIMGTSATSRRLERGLHLISLAASPNDTTLGEALASIAGKYSYVAGFDSR
                     WGGARTYVDSLADYSDLTSLDGVHGYWIRMEEAGDLAPAAARVHDDTPISLNVGWNLI
                     GYLPVTARAPADALATVLERVRVVGGFENGAKTFVPGSPFSDLTVLRHAYGYWVSMSA
                     PATLVWRPGAAIAQPPHAKAALMAEEPPPTREWADFYGTLTVDGVPVPAGTEVLLRDP
                     DGIVCARTVTRAAGRYGFLHAYGDDPATDADEGARPGDALRVIVDGREYPGKMAVWTG
                     SLSVTRVDLALSSAPTAVSEARPARFAFDGPQPNPFNPSVLLRFELATRGHTRMEVFD
                     GLGRHVRTVISADLGPGRHEATWDGVDENGRAVASGLYVIRLSAPDGVITRRAVLAR"
     gene            4319..>4996
                     /locus_tag="PLT86_12540"
     CDS             4319..>4996
                     /locus_tag="PLT86_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37481.1"
                     /translation="MHRPTAYGVLAAALVLGGPAAALSPSQYWRDYFSPSSMGPDGRP
                     LAAGSLVQVFDPDGVECGRFVVVTPGQYGYLHVYGDDPLTPEDEGAVPGDSLRFMVDG
                     SECVEVPAAVWESGDRLQHRVDLYPGSGFFLYGTVRYAGGIAVSGISFTVTGENALAQ
                     ATSGEDGIFVAGPFSVGTHRVGVTITGETPFRPAVTAFDAAQVLRAVVTGAPLGAPMA
                     VADVTGDG"
BASE COUNT          870 a         1628 c         1584 g          914 t
ORIGIN      
        1 tcgtccgtgc gtgtccgctt tgtgacggac gacaacatcg acgcgggaaa cacgagttac
       61 aaccgccaat cgggcggctg gtggctcgat gaaatccgtg tcgaggagct tcccgctccc
      121 gtaacgctca accccatcac ctcgagctcc cagcatcacg cggcgctctc atggacacag
      181 aacgcggata ccgacttcag ccggtacgaa atccgccgcg ccacgagcag cgccgtgacg
      241 atatccagcg acctcgtgaa aaccatcacc acccagtcaa caacggcgtg gaccgacacg
      301 tttgccttgg tggaaccggc acggttctcg tatcgagtat atgtcgttaa cacactggaa
      361 aacgtgagcc ctggtagcaa tattcttacc gccgactaca cgattccgct gaagacattc
      421 cccttcaccg acgatatgtc cgcggccacg gaaaactgga gctggggcga cccgtggggt
      481 cccacatcgg aatcgtttca ttccggcccc gcaagctgga aaagcgggcc gggacgagcg
      541 tacccgccga atgcgaacac tgcgctcgcc acccgtgtaa acctctccgg ggccacggcg
      601 cccgttctca ccttctggca tcggtacgct tttgagcagg cggtggactt cggctacgtg
      661 gaaatctcgt cagacggcgg gcagacatgg gctgccgtca tggcggtcac cggggtggat
      721 accatgtggg ccagtgagcg gatagatctc ggggcttaca tgggcagcac gatcggtctc
      781 cggttccgcg taacaagcaa cgcaaccacg cacttggacg gctggtacat cgacgacgtc
      841 accatcgcga atggccctcg catggcgacc tatccgctct tcgacgacat ggaatcgggc
      901 ccgaacgcgt ggttcccgca gtcgccgtgg gggctctcca atgtcggctc gcgatccggc
      961 gaatggcact ggacggacag cccggcggga agttacgcgc ccaacacgaa ctccgcgctc
     1021 accttgcgca tcaatctctc aggcgccgcc gcacccgaac tccgtttctg gcagcgctac
     1081 tcattcgagc ccaacgcgga ctggggatac gtcgaagtgt ccggcaacaa cggctcttcg
     1141 tggacccagg cctgcttcgt caccggcagc caggcaagct ggcgcgaaga acgggttgat
     1201 ctttccccgt ggaagggcaa cgcggacgta ctcctccgct tccgcctcac gtcgaacgcc
     1261 tccaacgaaa gcgacggatg gcacatcgac gacatctcca tggttgaggt cgcgagggag
     1321 ttcccgtacc cgttcgtgga aacgtttgac gacacggctg ccctttcccg ttggcacacg
     1381 gcaagttggg aaccggtcac cggtggccgg accacgccgt tccagatgca cgacagccct
     1441 tccggaaact accccgccaa tgcgttcagc gctttgacgc tcgccggcac gatcgacctt
     1501 cgcggcgcaa cgaaccccct gctgacattc tggcacaaat acgcattttt ttcggatggt
     1561 tcgtatgggc ataacgaata cgactacggg cgcgtgtacg tcagcccgga taacggagca
     1621 gtgtggtacc tcgtcagctc ttacaacacc tccgcaacga gctggaccaa agcttctctc
     1681 aacctcaacc agttcgtcgg tgcctcggca gtgcgcgtcc ggttcgtgat ggatgacgac
     1741 gtagacgcag ccaattccag ctacaaccgt caagccgacg gctggtggat agacgacgtg
     1801 cgcatcggcg agaacctcgc cgtgcaggcc cttgccgatt ccgtccgcct cgaagggccc
     1861 gcgttggcgt tcgcggttcc cggcctcgcc agtccgcgct acatcggccg catctacgag
     1921 ccatccgtca ccagcactgc cgggcagggc gccaacgtcg tcggccagtt cggagtgggg
     1981 ccacgcggca ccatgcccaa cgacacgtcg tggacatggt tcgccgccgg gtacctcgat
     2041 gacgtctcca gcttcgaccg gttcttcggc tcggtccgcg tcgattcctc cggcgagttc
     2101 gatgtcgcct tccgcggctc cattgacggc ggcgcgacgt gggtgtacgc ggatctcgat
     2161 ggcaacaacc tcgccggcgg cggggtgaac gagtacacca ccgcgcaggc tgcccggctt
     2221 atcgtcgggc gcggcgcctc cgtacggctg gatgtcgcca cggtagaacg cggtatccga
     2281 accggcacgc aggagttctg gccgttcacc ttttccaatg gcggccccga cgttctcgcg
     2341 tggtggctcg ccgaagcgga cagcaatcag gcggtcaagg acatcccctg gctggtgata
     2401 ggcaagagct ccggcacact ggctgagggc ggacgtgcgg tggtagacct caccttctcg
     2461 gctgtcgggc tgaatcccgg cctctacaaa gccaacctca tgatggtctc caacgacgcc
     2521 ttgaacgaca ccatcgcaat cccggtgcgg ctcaccgtat acgcaacagg cgtcacggcg
     2581 ttcacgggac gcgttgcgtg gacgggcggc ggaaatgtgg ccggcggaac catcgtcgca
     2641 tcgcaatcga accttccggt cgatacaatc gcggtctccg aaaacggtca gttcgccgcg
     2701 tacggcattc ccgccggtca gtacgatctc gccgtgcagg ttcccggcgc gtaccctcga
     2761 gcgttccgca acttcaccct ccccgcggaa aacgcgctcc tcacggtaac accaattcac
     2821 ccgcggatgg tgagcacgtc cttcgccgat ttctacagcc ccgcctcgac gcttgacggg
     2881 cagccactcg cggttggcag tgtggtttcg gtgcgcaatg cagccggtac cgtctgtggc
     2941 tcctgcgtgg cgactgaacc cggcaaatac ggcctgctcc acgtgtacgc agacgacccc
     3001 tccacgccgg aaacggaggg cgcctccatc ggcgacacac tcgcgttctt cgtgaacgat
     3061 gtgccgaccg gcgcgaaggc tgtctacacg gcccacttca gcgtcactcg cgtagatatc
     3121 atgggaacgt ccgccacgtc tcgccgtctc gagcgaggcc tgcatctcat ttcgctggcc
     3181 gcttctccga acgacacgac gcttggcgag gcgctcgcat ccatcgccgg caagtattcg
     3241 tacgtcgccg gttttgatag ccgctggggc ggcgcgcgca cctacgtgga ttcgctggcg
     3301 gattacagtg atctcacctc cctcgatggc gtgcacggct actggatccg gatggaagaa
     3361 gcgggcgatc tggctcctgc cgccgcccgc gtgcacgacg acacaccgat ttccctgaac
     3421 gtcggctgga acctgatcgg ttaccttccg gtaaccgcac gcgctcccgc cgatgcgctg
     3481 gccacggtgc tggagagagt ccgtgtggtg ggcgggttcg agaacggggc gaagacgttc
     3541 gtgcccggca gtccgttcag cgatctcacc gtgctccgtc acgcgtacgg ttactgggtg
     3601 tccatgtctg ccccggcaac gctggtgtgg cggccgggtg ctgcgatcgc tcaacccccg
     3661 cacgcaaaag cggcgctgat ggcggaggag ccgcccccga caagggaatg ggctgacttc
     3721 tacggaacgc tgacggttga tggcgtgccg gttcccgccg ggacggaggt cctcttgcgc
     3781 gatccggacg gaattgtctg tgcgcggacg gtaacccgtg cggcaggcag gtacggcttc
     3841 cttcatgcgt acggggacga cccggcgacg gacgcggacg aaggtgcgcg tccgggtgac
     3901 gcgctgcgcg tgatcgtcga cggacgtgaa tatccgggga aaatggcggt ctggaccggc
     3961 tcgctctctg tgacacgggt cgatctggcg ctctcgtcgg cgccgaccgc ggtgagcgaa
     4021 gcacggccgg cgcggttcgc gttcgacggg ccacaaccca acccgtttaa tccctcggtg
     4081 ctgctgcgat ttgagctggc cacccgcgga catacgcgca tggaggtctt cgacggtctc
     4141 ggacgacacg tgcgcacggt tatcagcgca gatctcgggc cggggcgtca cgaggcaacg
     4201 tgggacggcg ttgacgaaaa cggtcgtgcc gttgccagcg gcctctacgt catccggttg
     4261 tcagcaccgg acggtgtgat cacccggaga gcggttcttg cgcgttagga gccactggat
     4321 gcaccgaccg acggcgtacg gcgtccttgc cgcggccctt gttctcgggg gaccggccgc
     4381 ggcgctttcc ccgagccagt actggcggga ttacttcagc ccgtccagta tgggcccgga
     4441 cggccgtccg cttgccgcag gttcgcttgt gcaggtgttc gatccggacg gggtggaatg
     4501 cggccggttc gttgtggtga ctcctggcca gtacgggtac ctccacgtgt acggggatga
     4561 cccgctcacg ccggaggacg aaggcgcggt accgggtgat tcgctccggt tcatggtgga
     4621 cggttccgaa tgcgtggagg tccctgccgc agtctgggag tcgggggaca ggctgcagca
     4681 tcgggtggat ctgtatcccg gttcggggtt cttcctgtat ggaaccgtcc gctatgccgg
     4741 aggaattgcg gtatccggca tatcattcac ggtcaccgga gaaaacgccc ttgcgcaggc
     4801 cacgagcggg gaggacggca ttttcgttgc agggcctttt tccgtgggaa cccaccgggt
     4861 cggtgtcacg atcacggggg agacgccttt ccgtccggca gtgactgcct ttgacgcggc
     4921 tcaggtgctt cgggcagttg tcacgggggc gccgctcggc gccccgatgg cggtggcgga
     4981 cgtcacaggc gacggc
//
LOCUS       DAOSOO010000221         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_16946_length_4996_cov_5.378264, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000221 DAOSOO010000000
VERSION     DAOSOO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16946_length_4996_cov_5.378264"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..836
                     /gene="grpE"
                     /locus_tag="PLT86_12545"
     CDS             141..836
                     /gene="grpE"
                     /locus_tag="PLT86_12545"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT37482.1"
                     /translation="MKVTIREEKPKDKHEEKGTVTEQAAEVSSPATGGTGSTCPEEET
                     RQEPASKVEEEAEKEALEQQLAGAQREAAENRDKYLRAVAELENFRRRTAREFAEMSL
                     RAGERILTALLDPADNLARALDAARKMEAERAGEALTSFIGGVEMIYQQLVSILEKEQ
                     VTPMETVGTTFDPNLHDALVAVERPDVPPNTVVEEVQRGYRLGDRVLRHAKVVVSKAP
                     VAEEKPNENENTQ"
     gene            878..2803
                     /gene="dnaK"
                     /locus_tag="PLT86_12550"
     CDS             878..2803
                     /gene="dnaK"
                     /locus_tag="PLT86_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT37483.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNQPVVITNTEGGRTTPSIVAFTG
                     DNERVVGQAAKRQMITNPENTIYSIKRFMGRRHSEVSQEEKLVPYKVTSAPNGDVRVV
                     ARGKEYAPPQISAMILEYLKKTAEDYLGTTVTEAVITVPAYFNDSQRQATKDAGQIAG
                     LDVKRIINEPTAASLAYGLDKKKNEKIAVFDLGGGTFDISILEVGDGVFEVLSTNGDT
                     HLGGDDFDKKLIDWMADEFLAKEGVDLRKDKTALQRLRDAAEKAKIELSSSLQTTINL
                     PFIAEGPKHLEMTLTRAKFDALTADLVERTVGPCRQALKDAGLTASQIDEVILVGGST
                     RTPSVQAKTKELFGKDPNKSVNPDEAVAVGAAIQGAVLAGDVKDVLLLDVTPLSLGIE
                     TLGGVMTTLIERNTTIPTRKSQIFSTATDGQPSVRVHVLQGERAMAAENRTLATFDLV
                     GIPPAPRGVPQIEVTFDIDANGIVHVTAKDLGTGKEQKIKVEVSSGLSKQEIDRMVKD
                     AEAHRDEDQKQRELIELRNQADSFVYQAEKNLKEYGDKVDATEREKIQTAIDGVKSVQ
                     NGSDAGAIRSAIETLQQAMMKLGEVMYQQTAGGAGGPGPEGAQPGTGSAGGPQSGSAG
                     GPVDAEFTVEDDDKKNS"
     gene            3008..4180
                     /gene="dnaJ"
                     /locus_tag="PLT86_12555"
     CDS             3008..4180
                     /gene="dnaJ"
                     /locus_tag="PLT86_12555"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT37484.1"
                     /translation="MAQKRDYYEVLGIERTANDDAVKKAYRKLALKYHPDRNPGDKAA
                     EEKFKELAEAYEVLSDPEKRQRYDRFGHQGVQDQFGQGGFQWQNFSHASEFEDIFSSF
                     FGSRGFEDIFGGGGTRTSRRRGAQQGSDVRVSLSLPLEEIATGVEKRIRLKRVQVRCP
                     TCNGTGGKEGSKPTTCVACGGSGQVRRMQGGFFNLVTVATCDRCNGSGQVVTDPCPTC
                     RGTGLTSDERTVSVRIPAGVANGNTMRLRGQGNAGPHGGPAGDLLIDIHEEEHPLFQR
                     QDDDVVYELPISFSQAALGGDVEVPTLDGKVRMKVPPGTQSGKVRRLRGKGIPHMNGY
                     GNGDMLVRLHVWTPVKLNSQERKLFEELARLEKSSESGTPAGGRSFFDKVRDAFRD"
     gene            complement(4194..>4996)
                     /locus_tag="PLT86_12560"
     CDS             complement(4194..>4996)
                     /locus_tag="PLT86_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37485.1"
                     /translation="LSPRYMWSYSSYAAWFAPTDKTRNIAGVRFPDFPGAEEAVSVWH
                     DILRDQTRYEEQPDADPRVLRLFDAVRFFRDGLCTSEELCDAFGTPGRWLVLGPLSNP
                     HSVPKRTADEALEQPIEAKRIFCGRDGAVRWFTWFSHDPRGIANTRSVFDFLAADNMS
                     MHLVAWLHSDREREVVLNVGHNDGVVVRVGDRVVLNKPELPAVPNRGFFRDRYQFEDF
                     VLVTIPEGKTRIAVTVIAGKDSWRFAFRVTDRDGYPVDGVRFTATFEG"
BASE COUNT         1143 a         1364 c         1673 g          816 t
ORIGIN      
        1 aacaaccctc cacgaaccgc acggcacagg tattgcgttt ctccatgccc cgaaggtttc
       61 ggtcaatcgt cggacagacg ttcgccggct gagattccaa gatagcaagc aacgtccatc
      121 ttatcgctca ggagacgcgt gtgaaggtta caattcgtga agaaaagccg aaggataagc
      181 acgaggaaaa gggcacggta accgagcaag ccgctgaagt gtcctctccc gcaaccggag
      241 gcacgggaag cacgtgcccg gaagaggaga cccggcagga accggcatcg aaggttgagg
      301 aagaagctga gaaagaagca ctggagcagc agcttgccgg ggcgcagcgt gaagccgcgg
      361 agaacaggga caagtacctg cgcgcggtgg cggaactgga gaatttccgc cggcgcaccg
      421 cccgcgaatt tgctgaaatg agcctgcggg ccggggaacg gattctcacg gcgcttctgg
      481 atccggcaga caatctggca cgtgctctgg atgcggcgag gaaaatggag gccgagcgag
      541 cgggagaggc gttgaccagc ttcatcggcg gggtggagat gatctatcag caactggtct
      601 caatccttga gaaggagcag gtcacgccga tggagaccgt cggcaccacg tttgatccaa
      661 accttcacga cgcgcttgtt gcggtggaac ggccggatgt tccaccgaat accgtcgtag
      721 aagaagtgca acggggatac cggctgggcg accgcgtgct gaggcacgcg aaggtggtcg
      781 tcagcaaggc accggttgca gaggaaaaac cgaacgagaa cgaaaacact caataaatct
      841 ctgagccgcc tgattcggcg gaagaaaggc ttgaaccatg ggaaaaatca taggaattga
      901 cctcgggact acgaacagct gtgttgccgt gatggagggc aaccagcctg tagtcatcac
      961 gaataccgaa ggcggacgaa caacaccgtc catcgtcgcg ttcaccgggg ataacgaacg
     1021 ggtggtcgga caggcagcaa agcgtcagat gataacgaac ccggaaaaca cgatctactc
     1081 catcaagcgg ttcatgggac gacggcactc cgaagtatct caggaggaga agctggtccc
     1141 ctacaaagtg acatcggcgc caaacggtga tgtccgcgtg gtagcgcggg gcaaagagta
     1201 tgcaccgccg cagatttcgg caatgattct tgagtatctg aagaagacgg cggaagacta
     1261 tctcggcacc acggtgacag aggctgtcat cacggttccc gcgtatttca atgactcgca
     1321 gcggcaggca accaaggatg cgggccagat cgccgggctg gatgtcaaac ggatcatcaa
     1381 cgaaccgact gcggcgagtc tggcatacgg tcttgacaag aagaagaacg aaaagattgc
     1441 tgtgttcgac ctcggtggtg gaacgttcga catttcgatt ctcgaagtgg gcgacggagt
     1501 gttcgaggtc ctttcgacga atggcgacac acatcttggc ggcgatgact tcgacaagaa
     1561 gcttatcgac tggatggccg acgagttcct ggcaaaagag ggcgttgacc tccggaagga
     1621 caaaacggcg ctgcagcggc tgcgtgacgc ggcagaaaag gccaagatag agttgtcgag
     1681 ttcgcttcag accacgatca acctgccgtt catcgcggag ggaccgaagc atctggaaat
     1741 gacgttgacc cgcgcgaaat tcgatgccct gacggcggac cttgtggaac ggacagtggg
     1801 gccgtgccgg caggctctga aggatgcagg cctcaccgca tcacagatcg acgaggtgat
     1861 tctggtcggt ggatccacgc ggacgccctc ggttcaagcg aagacgaaag agttgttcgg
     1921 caaggacccg aacaagagcg tcaacccgga cgaagcggtc gcggttggcg cggcgattca
     1981 gggcgcggtg ctggcgggtg acgtgaagga tgtgctgctg ctggacgtta ctcctctctc
     2041 gctgggtatc gagacgcttg gcggagtgat gacgaccctg atcgagcgga acacgacgat
     2101 cccgacgcgg aagagccaga tattctcgac cgccaccgac gggcagcctt cggtgcgcgt
     2161 gcatgttctg cagggtgagc gtgcgatggc ggcagagaac cggacgcttg caacgttcga
     2221 ccttgtcgga ataccgccgg cgcctcgcgg cgtgccgcag attgaggtca cctttgacat
     2281 cgacgcgaac ggtatcgtgc acgtaacggc aaaggacctc gggaccggca aggaacagaa
     2341 gatcaaggtc gaggtttcga gcggcctgag caagcaggaa atcgatcgga tggtcaagga
     2401 cgcggaggct cacagggacg aggaccagaa gcaacgcgag ttgatcgaac tgcgcaacca
     2461 ggctgattcg ttcgtgtatc aggcggaaaa gaacctcaaa gaatacggtg acaaggttga
     2521 cgcgaccgag cgggagaaga tccagaccgc cattgacggg gtgaagagcg tccagaatgg
     2581 gtctgacgcc ggcgcgatca ggtctgcaat cgaaacgctt cagcaggcga tgatgaagct
     2641 tggtgaggtg atgtatcaac agacggcggg cggggctggc ggcccgggtc cggaaggtgc
     2701 gcaaccggga acgggaagcg cgggcggccc gcaatcaggg tccgcaggcg gaccggtgga
     2761 cgccgagttc acggtcgagg acgacgacaa aaagaactcg taagtccccg tcggcgtagc
     2821 aaataccgat tcttcggcag aggggcggtt ctgtggacac gtactgacgt gctcggaaca
     2881 gcccctttgc ccgttgcttc ggagcctttt ccggtgggtc ccgcagggag cccgggagga
     2941 aacggcgcat gaacggagtt atcttttctt aagacgctgg aaacacggag accgcgaagg
     3001 acgatagatg gcacagaagc gtgattacta cgaagtactc ggcatcgagc ggactgcgaa
     3061 cgacgatgcc gtgaagaagg cgtatcggaa gctcgctctg aagtaccatc ccgatcggaa
     3121 ccctggcgac aaagcggccg aagagaagtt caaagagctt gcggaagcgt acgaggtgct
     3181 gagcgacccg gaaaagcggc agcggtatga cagattcggc catcaggggg ttcaggatca
     3241 gtttggtcag ggcgggttcc agtggcagaa tttttcgcat gcgagcgagt tcgaagacat
     3301 cttctcctcg tttttcggaa gcaggggatt tgaggacatt ttcggcggcg gtggcacgcg
     3361 cacgagccgg cgccgcggcg cgcagcaggg ttccgatgtg cgcgtatcgc tcagtcttcc
     3421 gctggaagag atcgccacgg gagtggagaa gcgtatcagg ctcaagcgtg tgcaggttcg
     3481 ctgccccacg tgcaacggca ccggcggcaa ggagggatcg aaaccgacca cgtgcgtcgc
     3541 gtgcggcggt tccgggcaag tccgacggat gcagggggga ttcttcaacc ttgtaaccgt
     3601 ggcaacctgc gaccggtgca acggttcggg acaggtggtg acggacccgt gcccgacgtg
     3661 tcggggaacc gggttgacga gcgacgaacg caccgtatcc gtccgtatcc ccgcgggcgt
     3721 tgcgaacggc aacaccatgc ggctgcgggg acaggggaat gcggggcccc acggggggcc
     3781 tgcgggcgac ctgctgatag acatccacga ggaagagcac ccgttgttcc agcggcagga
     3841 cgacgacgtg gtgtacgagc tgcccatcag cttcagccag gccgcgctgg gcggagacgt
     3901 ggaagtaccg acgctcgacg ggaaggtgcg aatgaaggtt ccgccgggaa cgcaatccgg
     3961 aaaggtgcgg aggttgcgcg ggaagggcat cccgcacatg aacggttacg ggaacggcga
     4021 catgctcgtg cgccttcacg tctggacgcc ggtgaagctg aactcgcagg aacggaagct
     4081 gttcgaggaa ctcgcccggc tggagaagag ctccgaatca gggactcccg ccggcgggcg
     4141 gagtttcttc gacaaagtac gtgacgcgtt ccgggattag agggggaagc gtatcacccc
     4201 tcgaacgttg ccgtgaaccg cacgccgtcc acgggatacc cgtcgcggtc ggtgacgcgg
     4261 aaggcaaaac gccatgagtc cttccccgcg atcacggtga ccgcgattcg cgtctttccc
     4321 tccggaatag tcaccaaaac gaagtcctcg aactggtagc ggtcccggaa gaacccccgg
     4381 tttggtacgg cgggcaattc gggcttgttc aacaccaccc gatcgcccac ccgcacgacc
     4441 actccatcat tgtgccccac gttgagcacg acctcccgct ctctgtcgct gtgcaaccac
     4501 gcgaccaagt gcatgctcat gttgtcggcc gccaggaaat cgaacacgga gcgggtattg
     4561 gcgatgccgc ggggatcgtg cgagaaccac gtgaaccatc gcaccgcgcc gtcgcgcccg
     4621 cagaaaatgc gtttggcttc gatcggttgc tccaacgctt cgtccgccgt tcgcttgggg
     4681 acactgtgcg ggttgctcag cgggcccagc acgagccacc ggcccggtgt tccgaaggcg
     4741 tcgcaaagct cctccgatgt gcaaaggcca tcgcggaaga agcgcacggc atcaaacagc
     4801 cgcagcacgc gcggatcggc gtcgggctgc tcctcgtagc gggtctggtc gcgcagaata
     4861 tcgtgccaga cggaaactgc ttcttccgct cccgggaaat cggggaagcg cacgccggcg
     4921 atgttgcgtg tcttgtcggt tggggcaaac cacgccgcgt aagatgagta cgaccacatg
     4981 taccgcgggg acaggg
//
LOCUS       DAOSOO010000222         4970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17052_length_4970_cov_4.852899, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000222 DAOSOO010000000
VERSION     DAOSOO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17052_length_4970_cov_4.852899"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..917
                     /locus_tag="PLT86_12565"
     CDS             243..917
                     /locus_tag="PLT86_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT37486.1"
                     /translation="MIYLVRAELPENRQTSLAGAIPLKIRLEEATGLPCLIRWFSDVS
                     LTEVRRLPVRAIVIAGFAADIETMGEAAFRPLTEAVMGAEVPIMGICGGHQLLGCMFG
                     GDPWQNFPMRPLRPGEPDVSDYHPGMFKEWGFHTVRKVGTDPLFDGLPDDMVFSQSHY
                     CEIRTLPPDFELIATNDNCRVQAMRHRSRPIWGTQFHWERFTDHYPHGRRVMENFFRE
                     AGIATS"
     gene            1374..3698
                     /locus_tag="PLT86_12570"
     CDS             1374..3698
                     /locus_tag="PLT86_12570"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOT37487.1"
                     /translation="MPAYRTRTAPITCIIGRDTPIANDMDRIIDQFRDAPPAMLLGGD
                     DNPYHPDGYGLDGLTPEKGRELIARMQEWNRKVYAAGVRYNFPYICNCHIYGRPDTRT
                     GMWYLYDHWDEHADIIGPKPAVEPEQWMQREPDGAPHHNYPYRWLLPDRYELEGCVNN
                     PHWDEWLQRSARFLVKLGYNGTFIDNNIHHCYCEHCQRAFHTYLEETYTAEERLARFG
                     TADVSGLKLETRGNKVLWARDQREYLERAKASDPEHFAKLMGTDDIDKCVPSEAGNGF
                     HWGRSHDYWVDSLRKQYSEEEVALILRTGDVSSLGIETPVQRCLWADTQKFWAWSIAK
                     WHDRLHRALDELCPDFILNPNWGAITGFRNVDSRRLEGKNVRLWANSQDIIFYEQNYL
                     PCTLAPGYTLDLVIAYKYSNAAGARASVLAYFGVQYRALAELAMAEAATWSSDGIFIE
                     ARYKFPETRNAYGQFYERHTGWYAGRTSHARVGILYDYDNVHMENTYHIREVYALAHY
                     CADNHILFDLFNEANVTVDELRRFDVVIIPHVEFLSTAGRKAILEYAAGGGNVLITGN
                     TGVFDEHGRPAVANDAIERLRAAVWPSTPYAREGSIAAIGDVFAWLPKRTKEIHDIVD
                     INPQGLFEKGVYEQIVEASKHETHDDPRLATILSELAGFDLRVLPNAPATLRVSAWAG
                     DDGSLVVHLLNYNVPVSSLYGNIHEIAPEQVEPVPVENAVVSLPIPDGMRVSSVEMAD
                     PWHPDPEPLPFVVEGGRVRFTVPRVDIYRVVRLR"
     gene            complement(3976..4455)
                     /locus_tag="PLT86_12575"
     CDS             complement(3976..4455)
                     /locus_tag="PLT86_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT37488.1"
                     /translation="MGTEKTTIGRALGQIPGGVFVVTAGKGEKGTGFLASWVQQAAFE
                     PPLISIAVKQGRPIQALIESGEPFAVHVLGKDQKKIAAHFAKGFAPGEAAFEGVGVRT
                     GVTGAPVLDGVNAYLECRFYGKTAAGDHVIYLAEVVAGAAVEGSEPEVRVRKDGFAY"
     gene            4648..>4970
                     /locus_tag="PLT86_12580"
     CDS             4648..>4970
                     /locus_tag="PLT86_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT37489.1"
                     /translation="MKDNVIVIFGASGDLTARKLVPALFNNFRKGRLPERTHIIGFSR
                     TRFTDDAFREKMRDAVQTFAPRDFSSGTWTDFAPLLHYQPGDLARHNDFSALEQLIHA
                     LTGGE"
BASE COUNT          949 a         1655 c         1454 g          912 t
ORIGIN      
        1 gttttctccc gaaccctcgc gccggagcct gtcgaagggc cgcgcaggca gcgccaacgg
       61 cgcgaaatac cgtagcccgg ctcgcagggc cgggcgcgtt gcacgacact atggtcttct
      121 tccatccgga cggcgcaggc cgcatcccat tgcctcgaag cgggctccgg atgttttttc
      181 aacctaccac tcacgtatca gcagagcaaa cggcaccatt cccctacaca tcaggagccg
      241 ccatgatcta ccttgtccgc gccgaactgc ccgagaaccg ccagacctcc ctcgccggtg
      301 ccattccgct caaaattcgc ctcgaggaag ccaccggcct gccgtgcctc atccgttggt
      361 tttccgacgt gtcactcacc gaagtgcgca ggcttcccgt ccgcgcaatc gtcatcgccg
      421 gattcgccgc cgatatcgag acgatgggcg aggcggcctt ccgcccgctg accgaggcgg
      481 taatgggtgc cgaagtaccg atcatgggca tctgcggcgg gcatcagctc ctcggctgca
      541 tgttcggcgg cgacccgtgg cagaacttcc ccatgcggcc gcttcgcccc ggcgagcccg
      601 acgtgagcga ctaccacccc ggcatgttca aggagtgggg attccacacc gtcagaaagg
      661 tcggcaccga cccgctcttc gatggcctgc ccgatgacat ggtgttttcg cagagccact
      721 actgcgagat caggacactt ccacccgact tcgaactcat cgccacgaac gacaactgcc
      781 gcgtgcaggc catgcgccat cgttcgcgcc cgatctgggg cacgcagttc cactgggagc
      841 gcttcaccga ccactacccg cacggccgca gggtgatgga gaactttttt cgggaagccg
      901 gtattgccac ttcatgacac aggcaaaacg tcgtcttcgt gtcaagtgtc tttgtgcaga
      961 agttgcggat tcgcaccagg attttttacc cgcggccaca cctcggtgcc ctggccaggc
     1021 aggggtgcta taggtcgtat agaacgtatc agaccatggg cggcccatgt accgcaccgg
     1081 tgcgtctcga tacatggaac cggtgagcgt cgcttcgcct ccgttccttc ccggttcgcc
     1141 actcgactac cggcgcccca cccttgggca gcgcgcactg aagtgagcac ggcggcaggg
     1201 gagtggactt aacaacgcac gcatatccgc gtcttgcgac gcctgttcga gctcgtcacc
     1261 ccacccccgc acttggccac agccggcctg ccgaagtatt ctcaacgagt cgcagccatc
     1321 cacatgcccc gtgaacagga caaccgcgat cagactttcc tcaggaggca cccatgccgg
     1381 cgtaccgcac tcgcaccgct cccatcacct gcatcatcgg tcgcgacacc ccgatcgcga
     1441 acgacatgga ccgcatcatt gaccagttcc gcgacgcccc tccggccatg ctgctgggcg
     1501 gagacgacaa tccctaccac cccgacggat acgggcttga cggactgacc ccggaaaagg
     1561 gacgggaact cattgcacgg atgcaggagt ggaaccgcaa agtgtacgcg gcgggcgtgc
     1621 ggtacaactt cccttacatc tgcaattgcc acatttacgg ccgccccgac acgcgtacgg
     1681 ggatgtggta cctctacgac cactgggacg aacacgcgga tatcatcggc ccgaagcccg
     1741 cggtggaacc cgagcagtgg atgcagcgcg agccggacgg cgcgccgcac cacaactacc
     1801 cctaccgctg gcttcttccc gatcggtatg aactcgaagg gtgcgtgaac aacccgcact
     1861 gggacgaatg gttgcagagg agcgcgcgtt ttctggtgaa gctgggctac aacggcacgt
     1921 tcattgacaa caacatccac cactgttact gcgagcactg ccagcgcgcg tttcacacgt
     1981 atctcgaaga gacgtacacg gctgaggagc gacttgcacg gttcggcacc gcggacgtct
     2041 cagggctcaa gctcgagaca cgtgggaaca aagtgctctg ggcgcgcgat cagcgggagt
     2101 acctcgaacg tgcaaaggcc agcgaccccg agcatttcgc gaagctcatg ggcaccgatg
     2161 acatagacaa gtgcgtgccg tctgaagccg ggaatggctt ccactgggga cgctcccacg
     2221 attactgggt ggacagcctg cggaagcagt attcggaaga ggaagtggcg ctcatcctcc
     2281 gcaccgggga tgtgtcgtcg ctcgggatcg aaaccccggt tcaacggtgc ctctgggcgg
     2341 atacgcagaa gttctgggcg tggtccatcg caaaatggca cgatcggctt cacagggcgc
     2401 ttgatgaact atgccccgac ttcatcctca acccgaactg gggcgccatc accgggttcc
     2461 ggaacgttga ctcacgccgg ctcgagggga agaatgtccg cctctgggcc aacagccagg
     2521 acatcatctt ctacgagcaa aactacctgc cctgcacgct ggcgccgggg tatacgctcg
     2581 acctcgtcat cgcgtacaaa tactcgaatg ccgcaggcgc tcgcgcgagc gtgctggcgt
     2641 acttcggcgt ccagtaccga gcgcttgcgg aactggcgat ggcagaggcc gccacgtggt
     2701 ccagcgacgg catcttcatc gaagcgcgtt acaaattccc ggaaacgcgg aatgcctacg
     2761 gccagttcta cgagcggcac accggctggt atgcaggccg cacgtcgcac gcccgggtgg
     2821 gcatcctcta cgactacgac aatgtgcaca tggagaacac ctaccacatc cgcgaggtat
     2881 acgcgctggc gcactactgc gcggataacc atatcctgtt cgacctgttc aacgaggcaa
     2941 acgtcactgt ggatgagctg cggcgattcg acgttgtcat catcccgcac gtcgagtttc
     3001 tgtcaactgc cggccggaag gcgattctgg agtacgcagc aggaggcggt aacgtgctta
     3061 tcaccggaaa caccggcgtg ttcgatgagc acggccgccc ggcggttgcg aatgacgcca
     3121 tcgaacggct tcgcgcggcg gtctggccga gtaccccgta cgcgcgggaa ggcagcatcg
     3181 ccgcaatcgg ggatgtgttc gcctggctgc cgaaacgcac gaaagagatc cacgatatcg
     3241 tcgacatcaa cccgcagggc ctgttcgaaa agggcgtgta tgagcaaatc gtcgaggcgt
     3301 cgaagcacga gacgcacgat gacccccgcc tcgcaaccat tctctccgag ctggcggggt
     3361 ttgatctgcg cgtcctgccc aacgcacctg cgacgcttcg cgtatccgcg tgggcaggcg
     3421 atgacggctc gctcgtggtg catcttctca actacaacgt gccggtctcc tcgctctacg
     3481 gcaatatcca cgagatcgcg ccggagcagg tggagccggt gccagtggag aatgcggtgg
     3541 tttcgttgcc catcccggac gggatgcgcg tttcaagcgt cgagatggcc gatccgtggc
     3601 atcctgatcc cgagccgttg ccgttcgtgg tggaaggcgg gcgcgtgcgg ttcacggtgc
     3661 cgcgggtcga tatctaccgg gtggtacgct tgaggtgatc gggggacgaa gacgcgacgc
     3721 agcggatggt gctggctcgt tagcactcgc cgggctcgct aacgctcgct ctggttcacc
     3781 aaagctcgtt ctggttcgct gacgctcacg tggtgccagg gtgccttttt cgcaggtagg
     3841 ggcgcttcgc gaagcgcccc tacggcattt ctggcgcgag agcaggggcc tgccccccac
     3901 gcgacgtttc gccaggcttc cggcgcgatg cgcacccctt gcttcgtgtc cttcgcgcgc
     3961 taccttcccc gcgcttcagt acgcgaaccc gtccttgcga acccgcacct ccggctcaga
     4021 accttccact gcggcgcctg cgaccacctc ggcgaggtag atcacatggt ctcccgccgc
     4081 ggtctttccg taaaacctgc actcgagata cgcgttcacg ccgtcaagaa cgggagcgcc
     4141 ggtaacgccc gtccgtactc ccaccccctc aaacgcagct tctccgggcg cgaacccctt
     4201 cgcgaagtgc gcggcgatct tcttctggtc cttgcccaga acatgaaccg caaacggctc
     4261 tccggattca atcagcgcct gaatcggcct cccctgtttc accgcgatgc tgataagcgg
     4321 cggctcaaat gctgcctgct ggacccacga cgcgagaaaa ccggtgcctt tctcgccttt
     4381 tcctgcggtc acaacaaaga ctccgccggg gatctgcccc agcgccctgc cgattgtcgt
     4441 cttttccgtt cccatcgcac gtctccctat cactctgact gtgaggtgtg aattcgttcc
     4501 tccaaacgcc tgcgcggccg aacgccggtt gtcgtaccgg ctcagttcac tcaggcgcca
     4561 aaccgtgtta cttttaccaa acgccgcggg atgtgggaaa gttcaccgag ctgacgcaga
     4621 cctgcgcctc ggagggtagg taaggccatg aaggacaacg tcattgtgat attcggagcc
     4681 tccggcgatc tcaccgcccg gaagctcgtc ccggcgctgt tcaacaactt ccgcaaaggg
     4741 cgcctgccgg aacggacgca catcatcggc ttttcccgaa cgcgtttcac cgacgacgcc
     4801 tttcgtgaaa agatgaggga cgcagtccag acgttcgcgc cgcgggattt ctcgtccggc
     4861 acctggacgg acttcgcacc ccttctccat taccaacccg gcgaccttgc ccggcacaac
     4921 gacttcagcg cgctggagca gcttatccac gcgcttacgg gcggcgagtg
//
LOCUS       DAOSOO010000223         4898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17385_length_4898_cov_5.201941, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000223 DAOSOO010000000
VERSION     DAOSOO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17385_length_4898_cov_5.201941"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLT86_12585"
     CDS             complement(<1..982)
                     /locus_tag="PLT86_12585"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOT37490.1"
                     /translation="MVPRQSRSDALRHLVLLPCRTKLVIADSHDVTGGNNDGFTSAYS
                     YQYRTPEGWVLFGTHQSGTVQLIRTIGFRTALKVFRGDSGEPDLVIPFAEIYSGTHPD
                     FPLPYVGEEESAHGSSWCFVPIPFENGCRIVAPEKEESYHFFNVWAHIYTEDTPEWEK
                     AADRVLWSSEEETAAASPACSGAGSLDAHGRQVLFEQSTPGLVTSIRVRLPEERRSEV
                     LRDVHLRAFWDGREAPDVDAPLGLFFGVGYPDDEALRRTPSYPLGEGEKSVEIPTGRV
                     APCALPVSEEPDGTLVCRFPMPFDSARIELVNTSAHSAGPIAWQLSVEPLP"
     gene            1190..3658
                     /locus_tag="PLT86_12590"
     CDS             1190..3658
                     /locus_tag="PLT86_12590"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT37491.1"
                     /translation="MSVARILEDLRKPAPFVRPMPFWFWNGKLDADLFIKQLDDMAEK
                     GIGDVFLHPMPQEFRPKDFVSGIEGEYLGREFMAAVRAVVEAAAQRGMRFWLYDEGGW
                     PSGVNLGRVVAARPDLKGKVARYNEEGNVEFLERGYPVDLLDPETTQTFISQVHEEYA
                     KVVGEFFGTTIPGIFTDEPRYGGRVGGAEIPWTPKLPEIFKEKTGYDISLALPILFGL
                     KVARSLPENKRAQVQADFYRVITELWRDSYFKPLHDWCAAHNLLLVGHLSGEDDLPSQ
                     LLFCGNFFTEMEYFDWPGIDVIWRQIFPGNRGNHFPKYASSAAHTRGKRWSLSESFAV
                     YGWDLTYAQMKWITDYQYVRGINTLVPMALYSDTRGARKIGTMSDQFQANPLWEHYRP
                     YAEYVGRLGGLLSAGKPVVEVGLYYPIVSLWAGEDARSVEAKVTRIATSLLDQQIDFD
                     FVDDAAISRAEISGGTMAMGDCCYSAIVVPNAPVMPVETMRRLVEFAKAGGSVTFYET
                     PPHFAARAADEAELAQLANSLSTCVASSADWDVALGMLPWTVKLAVHNANIRAIRRSC
                     EGTEIFFLTNESLTEDASLRLRLPVQGAVVAYDPDTEQTFDASPQGGFIRVTVRANGC
                     MVLLAGNTEGLTLAPPLDPLTRAQEVSGPWIATVTRQWAFRGGEIVMLSAVKGMDRDA
                     GGVLGTPKRVDTLEDWDKVYYENFCGTVDYATSLPLQAAPPRALLDLGTVGVVAEVDV
                     NGTIIGKRLWAPYTFDITSALKVGTNNIRISVTSTLFRLMTMPEIVGELKQRGWFNSY
                     AQKVSEFKGDRAPAGLIGPVRILT"
     gene            4081..>4898
                     /locus_tag="PLT86_12595"
     CDS             4081..>4898
                     /locus_tag="PLT86_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019375917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37492.1"
                     /translation="MPTRPNILFIMSDDHAAHAMSCYGSRINHTPNLDRIASEGMRLD
                     RCFCTNSICTPSRATILTGTYNHVNGVTTLDTPMDAGLQTFPKLLRESGYQTAVFGKW
                     HLGEHKSFEFCPTGFDDWAVLPGQGFYHNPEFIFKGPRGPVQRTVMGYVTDLITDFCL
                     DWLQNRDRERPFCLLYHHKAPHRHWNPDEKHAAMYLNEDIPEPDTLYDDYSNRASAAA
                     AAEMRVGVHMTQLDMKCEVPKNIPEREMRKWAYQRYIKDYLRVIASVDDNVGRVL"
BASE COUNT          948 a         1592 c         1467 g          891 t
ORIGIN      
        1 ctggcaacgg ttccacagaa agctgccacg cgattggtcc tgcggagtgc gcagaggtgt
       61 tcacgagttc gatacgcgcc gaatcaaacg gcatggggaa gcggcacacg agcgtcccgt
      121 ccggttcctc gctcaccggc aacgcgcatg gtgcaacccg cccggtcggt atctcgacgc
      181 tcttctcgcc ttcgccaagt gggtaggagg gcgtccgccg gagtgcctcg tcatccgggt
      241 atcccacccc gaaaaagagc cccagcgggg cgtccacatc cggggcttcc ctgccgtccc
      301 agaaggcgcg caggtgtaca tctctcaaga cctcgcttct ccgctcctcc ggaaggcgca
      361 cgcggatgga cgtcaccagc cccggtgtgc tctgttcgaa aagcacctgt ctgccgtggg
      421 catcaagcga cccggcgccg gagcacgcgg gactggcggc tgctgtttcc tcttcactgc
      481 tccagagcac acggtccgcc gccttctccc actccggcgt gtcttccgtg tagatatgcg
      541 cccacacgtt gaaaaagtgg tacgattcct ccttttcggg cgccacaatc cgacacccgt
      601 tttcgaacgg gataggcacg aagcaccatg agcttccgtg cgcggactcc tcctcgccga
      661 cgtacggcag cgggaaatcc ggatgcgtgc cggagtatat ctccgcgaac ggaatcacga
      721 gatccggctc cccggaatca cccctgaaca ccttcagcgc tgtgcggaag cctatcgtcc
      781 gaatcagttg tacggttccg ctctggtgcg ttccaaacag cacccagccc tctggcgtgc
      841 ggtactggta ggaataggcg ctcgtgaaac catcgttgtt gccacccgta acgtcgtggc
      901 tgtcggcaat gacgagcttt gtacggcacg gaagcagcac gaggtgccgg agtgcgtccg
      961 aacgggattg acggggaacc atctgcgatg cctttcacga accgtgtccc tgatgcgccg
     1021 gcaaactgcc gccattggta acctaccgcc tccacgccgc cgccgcaaac cgccagtcgg
     1081 tcggcccgca tgcaggaagg cttgacatgg cttcgcattg caaggatttt gactatcaag
     1141 aacatacgta cctctccgcg tcatattccg tcatgtaagg agcgtcggcg tgagcgtagc
     1201 acgaatcctg gaagacctcc ggaagcccgc cccgttcgtg cggccgatgc cgttctggtt
     1261 ttggaacgga aaactcgacg ccgatctctt catcaaacag cttgatgaca tggcagagaa
     1321 aggcatcggc gacgtattcc tccacccgat gccgcaggaa ttccgcccca aggacttcgt
     1381 ctcgggaatc gaaggggagt acctcggcag ggagttcatg gctgccgtgc gcgcggtggt
     1441 ggaagcggcc gcgcaacggg gtatgcgttt ctggctgtac gacgagggcg gatggccgtc
     1501 cggcgtcaac ctcgggcgcg tagtggcggc aaggcccgac ctgaagggga aggttgcccg
     1561 ttacaacgag gaaggcaatg tagagttcct cgaacgcggt tacccggtgg accttctcga
     1621 cccggagacc acgcagacgt ttatcagcca ggtgcacgag gaatacgcga aagtcgtcgg
     1681 cgagttcttc ggcaccacca tccccggcat tttcaccgac gagccccgtt acggcggccg
     1741 cgtgggcgga gccgaaattc cgtggacacc gaagttgccg gagatcttca aggagaagac
     1801 cgggtacgac atttcccttg cccttcccat cctgttcggg ctcaaagttg cccgcagcct
     1861 gcccgaaaac aagcgcgcgc aggttcaggc cgatttctac cgcgtgatca ccgaactctg
     1921 gcgggatagc tatttcaagc ctcttcacga ttggtgcgcg gcccataatc tgcttctcgt
     1981 cggccacctt tccggggagg acgacctccc cagccaactg cttttctgcg gcaacttctt
     2041 caccgagatg gaatactttg actggcccgg tatcgacgtg atctggcgcc agatattccc
     2101 cggcaaccgg ggcaaccact tccccaaata cgcaagctcg gccgcccaca cccgcggaaa
     2161 gcggtggagc ctttccgaat cgtttgccgt atacgggtgg gatctcacct acgcgcagat
     2221 gaagtggatt accgattacc agtatgttcg cgggattaac accctcgtgc cgatggcgct
     2281 ctacagcgat acccgcggtg cccggaaaat cggcaccatg tccgaccagt ttcaggcgaa
     2341 tcctctctgg gagcactacc gcccgtatgc cgagtacgtg ggcaggcttg gcgggctgct
     2401 ctccgcggga aaaccggtgg tcgaagtagg cctgtattat cccatcgtca gcctgtgggc
     2461 cggcgaagac gcgcggtccg tggaggccaa ggtcaccaga atcgccacca gcctcctcga
     2521 ccagcagata gacttcgact ttgtggacga cgctgcgatc tcccgggcgg agatttccgg
     2581 cggaacgatg gcaatgggcg attgctgcta ctcggcgatc gtcgttccca acgcgccggt
     2641 aatgccggtg gaaacaatgc gcaggctggt ggaattcgcc aaggcgggcg gatcggtcac
     2701 cttctacgaa acaccgccgc acttcgccgc gcgcgccgcc gatgaggccg aactggcaca
     2761 gctcgcgaac tcgctctcca cctgcgtcgc gtcatcagcc gactgggatg ttgccctggg
     2821 catgcttccg tggacggtga agcttgccgt gcacaacgcc aacatccggg cgattcgccg
     2881 gtcctgcgag ggcacggaaa tcttcttcct caccaacgaa tcgctcacgg aggacgcctc
     2941 gctgcgcctc cgcctgccgg tgcagggagc agtcgtggcg tacgaccccg acacggagca
     3001 gacgttcgat gcttccccgc agggcggttt catccgcgtg acggtccgcg cgaacgggtg
     3061 catggtgttg ctcgccggga acacggaggg ccttaccctc gcgccgccac tggacccgct
     3121 gacccgtgcg caggaggtct ccggcccgtg gattgccacc gtaaccagac agtgggcgtt
     3181 ccgcggaggc gagattgtca tgctctcggc agtgaaaggt atggatcgcg acgccggcgg
     3241 cgttctcggc acgccgaaac gggtggacac gctcgaggac tgggacaagg tctactacga
     3301 aaacttctgc ggaacggtgg attatgccac ctcgctgccg ctgcaggcgg cgcccccacg
     3361 cgcactgctc gatctcggaa ccgtcggcgt agtggcggaa gtcgacgtga acggaacgat
     3421 tatcggcaaa cgcctgtggg cgccctacac gttcgatatc acctccgcgc tgaaggtcgg
     3481 cacaaacaac atccgcatat ccgttaccag caccctgttt cgcctgatga ccatgccgga
     3541 gatagtcggt gaactgaaac agcgcggctg gttcaacagt tacgcgcaga aggtctccga
     3601 gttcaaaggt gaccgcgccc ccgccgggtt aataggcccc gtacgcatcc tcacttgaac
     3661 catgcccagg cggggcaccc caccgggtta gtaggccccg tacggcttct taccgggtag
     3721 cctaccgggg gtgggacagg cattcttgcc tgtccgttgc tggcatgaaa ggcgaggcgg
     3781 gacatcccgc ttccgggccc ctgtgtgccg tcattcccgc agcctccatg ccacggggag
     3841 acccgcgttg tcgccgcacg atgaccggtc atcgagtggc gagcgcgtgg tccctgagcg
     3901 cgctcccgtc ggcgggagcc tgtcgaagtg cagcgctcga tgtatcgaga tgatcacgcc
     3961 aatacggcca atcggtgttc gatctctccg aacccggttg gccgcgcaca ccgaattcca
     4021 gaaatttgct actgaatcca tccggttacc gcggccgtcc ccttcgagga gtctgcaccc
     4081 atgcctacgc gtccgaatat cctgttcatc atgtcggatg accacgccgc gcacgcaatg
     4141 agctgctacg gcagccgcat caaccacacg ccgaacctcg accgcatcgc cagcgaaggc
     4201 atgcggctcg accgctgctt ctgcaccaac tccatctgca cgccgagccg ggcaaccatc
     4261 ctcaccggca cgtacaacca cgtgaacggg gtcaccacgc tggatacacc tatggacgcc
     4321 ggcctgcaga cgtttccaaa gctgttgcgg gagagcggtt atcagacggc ggtgttcggg
     4381 aaatggcacc tcggtgagca caaaagcttt gagttctgcc ccaccggctt cgacgactgg
     4441 gccgtgttgc cggggcaggg gttttaccac aatcccgaat tcattttcaa ggggccccgg
     4501 ggaccggtgc aacgcacggt gatggggtac gtgacggacc tgatcaccga cttctgcctc
     4561 gactggctgc agaaccgcga ccgcgaacgg cccttctgcc tgctctacca tcacaaggca
     4621 ccccaccggc actggaaccc cgacgaaaaa cacgccgcga tgtacctgaa cgaggacatc
     4681 ccggaacccg acacgctcta tgacgactac tccaaccggg cttccgccgc tgccgccgcg
     4741 gagatgcgcg tgggcgtgca catgacccag ctcgacatga agtgcgaagt gccgaagaac
     4801 attccggagc gcgagatgcg gaagtgggcg taccagcgct acatcaagga ctacctgcgc
     4861 gtcatcgcca gtgtggacga caacgtcggc cgcgtgct
//
LOCUS       DAOSOO010000224         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17496_length_4868_cov_5.139622, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000224 DAOSOO010000000
VERSION     DAOSOO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_4868_cov_5.139622"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLT86_12600"
     CDS             <1..526
                     /locus_tag="PLT86_12600"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOT37493.1"
                     /translation="SESGYGVSLLNDCKYGFDIHENVMRISLLRSAIRPDPKADEGHH
                     EFTYSLYPHAGSWRDANTVHRAYELNVPLIARVVPPNKGAKQANESFVRVDRNSVVID
                     TVKKAEDSDAVIIRCYEAHGVRGPVTMSFASPPQQVSECDLMEENDVPVRISGSSFEF
                     SIKPWEIRSFKVVF"
     gene            827..1252
                     /gene="ndk"
                     /locus_tag="PLT86_12605"
     CDS             827..1252
                     /gene="ndk"
                     /locus_tag="PLT86_12605"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT37494.1"
                     /translation="MDGTLMLVKPDAMQKGALGEILSAVQKEGFTVRNLKMLRLRAAE
                     AKEFYSEHRERPFFNDLVEFMTSGEIAAIALERNDAVATLRKLIGATDSRLAEPGTIR
                     ARFGTDNQMNAVHASDSISSARRELAFFFSTRELIPLPD"
     gene            1284..2234
                     /locus_tag="PLT86_12610"
     CDS             1284..2234
                     /locus_tag="PLT86_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOT37495.1"
                     /translation="MAEQVSTSLDSLGLTRIGGHGSSGAVNRVAVVGGGAMGQGLAQA
                     VSQTGIEVILVERNERTLEAAIEGLSRSLDKEIDKWGLTSSEKRAVLARVIGTTDLGQ
                     TARAPFVIEAIQEKAEDKATLLRDLDAICAEQAVLATSATSLSVTCIAEGVTRRERVI
                     GTHFLDPAPRVPLVEVVRGKHTSDATFVKAKNFIEKQLKKTVVEVADKPGGITTRVLL
                     ALLIEGFRCLTDEVASPEQIDTAVRLGWGFPVGPLEFADQMGLDRVLVWMQNLSREPG
                     GERFHPCPILVDLVNKGHCGKKAGRGFFEYDPEGRKLVAS"
     gene            2359..3129
                     /locus_tag="PLT86_12615"
     CDS             2359..3129
                     /locus_tag="PLT86_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOT37496.1"
                     /translation="MLVRGKRFLITGVADQNSIAWGITESLMREGAEGLAFLYQPRFE
                     RNVRELTAGIEGALLLEADISAPESLHAAFQTLENHWQDLHGMVHCIAAAKREELRGS
                     FSQTSRDGFLFAHEISAYSLLDLARRAVPLMERNGGGSILCLSYLGGQRVVPNYNVMG
                     VAKASLEMAMRYLAAELGPKSIRVNALSPAPIRTISARVISGVSEMFQRMEERSPMRR
                     NVTRGEVGDAALFFLSDLSRAVTGSVLFVDNGYHIMGA"
     gene            3143..4006
                     /gene="nadC"
                     /locus_tag="PLT86_12620"
     CDS             3143..4006
                     /gene="nadC"
                     /locus_tag="PLT86_12620"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT37497.1"
                     /translation="MTEFPQGYVHALVQRALEEDVGTGDITSAATVPESTVARAHLVA
                     KAAGVLSGVDVAEATFLAIDPGIRFSTCAGEGCRVAKGDLVFTVEGRARALLAAERTA
                     LNFLAHLSGVATLTARFVDAVAGSGARILDTRKTTPGMRLLEKRAVRSGGGMNHRVGL
                     YDMVLMKENHIRAAGGITKAISACNQYLRHQGLTGTKVEVETTCEEEVREALAAGCDR
                     IMLDNMTEDEMRSSVLLIRSIAPATEIEASGNMTLERVPAVAKTGVDFISIGALTHSA
                     PVLDLSLQFEM"
     gene            4054..4863
                     /locus_tag="PLT86_12625"
     CDS             4054..4863
                     /locus_tag="PLT86_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT37498.1"
                     /translation="MTRALGRTHFAFGSVTTTMELASRLAKAGAPHGSIVTAEQQTAG
                     RGRRGRKWISEPGLGIWCSVLLRTQECAGPAAPALGLIAACSVVAAARMNGVLCAGVK
                     WPNDVCALEHNAGAHCGESPVRWPSAWVLSARKIAGILVETSKELSGCFVVGIGVNVH
                     HSRSDFPSEFASSASSVDLCAGRDVARPALLADVLCCFEMRLGWSEAMVRSEWHRESL
                     TIGRRVKVSGPDGSFIGQAEAIEADGALKVSGPSGTVTVTGGETTVRCVSD"
BASE COUNT         1019 a         1286 c         1586 g          977 t
ORIGIN      
        1 ttccgaatcg gggtacggcg tcagtctact gaacgattgc aaatacggtt ttgacatcca
       61 cgagaatgtc atgcggatca gcctcttgcg gtcggcgata cgtcctgatc caaaggcgga
      121 cgaaggacat catgagttta cctacagctt gtatccgcat gctggttcgt ggcgagatgc
      181 gaatacggtg caccgggcgt acgaactgaa cgtgccactc atcgcgcgcg tggtgccgcc
      241 gaacaaaggc gcaaaacagg cgaacgagag ctttgtgcgc gtagaccgga acagcgtggt
      301 gatagacacg gtgaagaagg cagaagactc ggacgcagtg atcatccgtt gctatgaagc
      361 gcacggcgtc cgggggcctg ttaccatgtc gttcgcgagt ccgccgcaac aggtcagcga
      421 atgtgatctg atggaggaaa atgacgttcc ggtgaggatt tccggttcgt ccttcgagtt
      481 ctcaatcaaa ccatgggaaa tccgctcatt caaagtggtg ttctgaccga aggatccgtt
      541 gacgggaggg gagcaccgcg ggtggtttcc ctccctcacg ccctttcgaa tgccgtgcac
      601 aagccgagga aaacaaacgg cgagcctgcg gaagccagtg cgacgttgat cgcgcacctg
      661 caggtcggag tgtcgtgcgt gcttgttgac cggcccggcc gaccccgata ccttgaatca
      721 ttctggcgcg agaaacgtgt ccagtcgttt ttcgcgcgga accgccccga acccgactgg
      781 tgcccagcgc cgtcgcggga atcaagggga aacacaggag aaagacatgg acggcacgct
      841 gatgcttgtc aaacccgacg cgatgcagaa aggggccttg ggcgagatcc tctccgcagt
      901 gcagaaggag ggtttcacgg tccgcaatct caagatgctc cgcctccggg ctgcggaagc
      961 gaaggagttc tattcggagc accgcgaacg cccgttcttc aacgatctgg tggagttcat
     1021 gacgagcggc gaaatagccg ccatcgcttt ggaacggaat gatgctgtcg ccacgttgag
     1081 gaagttgatc ggggcgacgg attcccggct tgcggaaccc ggaaccatac gcgcacgttt
     1141 cgggaccgac aaccaaatga atgcggtgca cgcgagcgac agcatcagca gtgcacggcg
     1201 ggaacttgcg tttttctttt cgacacggga actaatcccg ttgcctgatt gacgaagtta
     1261 ttctccagac aggagtgtat tccgtggcgg aacaggtgtc cacctcgctg gattccctcg
     1321 ggttgactcg tatcgggggg catggcagtt cgggtgcggt taatcgcgtg gccgtggttg
     1381 gtggcggagc aatgggccag ggcttggctc aggccgtttc ccagaccggg atcgaggtga
     1441 ttctggtaga acgcaatgaa cgaaccttgg aagcggcaat tgaaggtctt tccaggagcc
     1501 tcgacaaaga gattgacaag tggggcctta cgagttcaga aaagcgggcg gttcttgccc
     1561 gcgtgatcgg aacgacagat ctcggacaaa ccgcaagggc tccgtttgtt atcgaagcta
     1621 ttcaggaaaa agcggaagac aaagcaacgc tcctgcgcga tctggacgcc atttgcgctg
     1681 aacaggcggt gctcgccaca agtgcgacgt cgttgagtgt cacctgtatc gcggaggggg
     1741 tgacgaggag ggaacgggtg atcggtactc acttccttga tcccgctccc cgggtgcctc
     1801 tcgtggaagt tgtgcgcggc aaacacacct cggacgcgac atttgtgaag gcaaagaact
     1861 tcattgaaaa gcaactgaag aaaacggtcg tcgaggtcgc cgacaagcca ggaggaatca
     1921 cgacgcgcgt tctgcttgcg ttgctgatag aagggttccg ctgtctcact gacgaagtcg
     1981 cttccccgga acaaatcgat accgcggttc gcctgggttg gggtttcccc gttggccctc
     2041 tcgaattcgc cgatcagatg gggctggacc gggttctcgt gtggatgcag aatctgtcgc
     2101 gggaacccgg cggcgagcga ttccacccgt gcccgattct tgtagatctt gtcaacaaag
     2161 ggcactgcgg aaaaaaagca gggcgcgggt ttttcgagta cgatccggaa ggcagaaagc
     2221 tggtcgccag ctagcggtgg cactggtttc tgcgggagta gcggaccgcg gcaattcctt
     2281 tcgagccgcg cgtgccggta cgtggtttgg tcggatctgg acagcacggc gcaacgtgga
     2341 attcgggagc gtgaggatat gctcgtacgc gggaaacggt tcctcatcac aggcgtggcg
     2401 gaccagaaca gtattgcatg gggcataacg gaaagcctca tgcgtgaagg agcggaaggc
     2461 cttgcgttct tgtatcaacc gcgatttgag aggaacgtcc gggagctcac cgcgggaata
     2521 gagggtgctc tgctgctgga ggccgatatt tctgctccag aatcgctgca tgctgccttt
     2581 cagactctcg aaaaccactg gcaggacctt cacgggatgg tacactgcat agccgcggcg
     2641 aagcgagagg aactgcgggg gagtttttct cagacttcgc gcgatgggtt ccttttcgcg
     2701 catgagatca gcgcatattc tcttcttgat ctggcaagaa gagcagttcc gttgatggaa
     2761 aggaacggcg gcgggtcgat actctgcctt tcgtacctcg gaggccagcg ggtcgttccg
     2821 aattacaacg tgatgggcgt tgcgaaggct tcacttgaaa tggcgatgcg gtaccttgcc
     2881 gccgaactcg ggccaaaatc aatccgggtc aacgcactca gcccggcgcc catacgtact
     2941 atttctgccc gcgttatctc aggagtctcg gagatgttcc agagaatgga agaacggtct
     3001 cccatgcgca ggaacgtgac gagaggcgag gtcggtgacg cggcgctttt cttcctgagt
     3061 gatctctccc gggcggttac cggttcggtt ctctttgtcg acaacggcta tcacataatg
     3121 ggcgcatgat gaggcaacgc cagtgacgga atttccgcag gggtacgttc acgcgctggt
     3181 tcaacgcgct ctggaagagg atgtcgggac aggcgatatc acgagtgccg cgaccgtgcc
     3241 tgaatcgacg gtggcgcggg cacacctggt ggccaaagcc gccggcgtgc tttctggagt
     3301 tgacgttgcc gaagcgacct tcctagcgat agaccccgga atcaggttct ccacctgcgc
     3361 aggtgaaggt tgccgggtgg cgaagggcga cctggtgttc acggtggaag gccgcgcccg
     3421 ggctctgctt gccgcagaac gaaccgcgct gaatttcctg gcgcacctgt caggcgtcgc
     3481 aacgctcacc gctcgctttg tggacgcggt ggcggggtcc ggagcccgta tactggacac
     3541 tcgcaaaacc actcccggca tgcgcttgct ggagaaacgc gcggtaagat ctgggggcgg
     3601 aatgaatcac cgcgttggcc tgtacgacat ggttctcatg aaagagaatc atatccgtgc
     3661 ggcgggtgga ataacgaaag cgatctctgc gtgcaaccag tatcttcgac accaggggct
     3721 gaccggaaca aaagtcgaag tggagacgac gtgcgaggag gaggtccgcg aagcgctggc
     3781 agcgggttgt gaccgaatca tgctggacaa catgaccgaa gatgagatgc gctcgtcggt
     3841 gctcttgatc cggtcgatcg cgcccgcgac cgagatcgag gcttcgggca acatgacgct
     3901 ggaacgggtt cccgccgtcg cgaaaaccgg cgtagacttc atttccatag gtgcgctcac
     3961 acacagcgcc ccagtccttg acctctccct tcaatttgag atgtgaagag aagtcctgat
     4021 ctcataacgc cggacgccct gagcggctgc atcgtgacac gggccctcgg tcgaacgcac
     4081 ttcgcgttcg gctctgtaac caccacgatg gagcttgcct ctcgtcttgc gaaggcggga
     4141 gcgccccacg gttcgatcgt tacggcagaa cagcagacag cgggacgggg ccggcgggga
     4201 cgaaaatgga tttcggaacc ggggctcgga atctggtgtt cagtgctgct gaggacccag
     4261 gagtgcgcgg ggccggccgc tccggcgctg gggctgattg cggcatgttc ggtggtggcg
     4321 gccgcaagaa tgaacggtgt actgtgcgcg ggtgtcaaat ggccgaatga cgtgtgcgcg
     4381 ttggaacaca acgcgggggc acattgcggc gagtcacccg tcagatggcc gagcgcgtgg
     4441 gtgctttccg cgaggaagat agccggcata cttgtcgaaa catcgaagga actgagcggt
     4501 tgtttcgtcg tcggaatagg ggtgaacgtc caccattcgc ggtcggactt cccatcggag
     4561 tttgcgtctt cagcatcgtc ggttgatctt tgtgcgggac gtgatgttgc acggccggcc
     4621 ctacttgctg atgttctttg ttgtttcgag atgagactgg gctggtccga agcgatggtg
     4681 cgttcggaat ggcatcggga atccctgacg atcggcaggc gagtcaaagt atccggcccg
     4741 gatgggagct tcatcggaca ggctgaagca atcgaggcag atggtgcttt gaaggtgagc
     4801 ggtccttcag gaacagtcac ggtaaccggc ggagagacca cggtgagatg cgtttccgac
     4861 tgaccgaa
//
LOCUS       DAOSOO010000225         4864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17512_length_4864_cov_6.081306, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000225 DAOSOO010000000
VERSION     DAOSOO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17512_length_4864_cov_6.081306"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLT86_12630"
     CDS             complement(<1..332)
                     /locus_tag="PLT86_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37499.1"
                     /translation="MAHYATDAELLRELPATLPESLDTEAERAPFIQSASALADALVG
                     PRFMPRDDGQKFPDSDDTPATPPLVAFATCRLAASFVYDVLAGLGDDTKRVRAGELFD
                     IALAWFAR"
     gene            complement(409..1362)
                     /locus_tag="PLT86_12635"
     CDS             complement(409..1362)
                     /locus_tag="PLT86_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HOT37500.1"
                     /translation="MALQSRPLAKDRFAEPADLHVDAALSHISLDFHNDEFIADRVSP
                     RIRVDKESDKYFVWGTEAFQTPTTGRTPKSLFARTEFAPATASFVCEEYGLEVPVDDR
                     ERRNADAGADIEIAAVEKATGIIMTCREKRLADILTSTSRVTQNTTLSGTSQWNDYTN
                     STPIANVDTARSTIHAAGAPNPNTLVMGREVFDTLKRHPDILEAFKYTRSGAVTEAMM
                     ADLLGVERVLVGNARYRTSNEGQASALAYVWGKNAVLLYTPPMPSRFTPAATYTFVFT
                     DRVVETYREPYKTCTVVRVREETAEVIASTACCYLIKNAIA"
     gene            complement(1966..2340)
                     /locus_tag="PLT86_12640"
     CDS             complement(1966..2340)
                     /locus_tag="PLT86_12640"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOT37501.1"
                     /translation="MAVDFGGQDITTVSGADLSAKRFYAVTMETTGKIALANSAGERC
                     IGVLQNAPSADGKLARVRVGGVSKVVVAAAINPGVELSATTAGKFSTATAGHYVMGIA
                     LEASGADGDIIRVLLTNYQKNA"
     gene            complement(2343..3326)
                     /locus_tag="PLT86_12645"
     CDS             complement(2343..3326)
                     /locus_tag="PLT86_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37502.1"
                     /translation="MPVFALGEHRGFRYDDAWARRALEVFQRLKRKYGYLPPVIIGHT
                     SDTESEKPAVGFMDGLRIAGNHLVADLVGLTTEVYDQIRAGRWPYRSIEVISRAAQIT
                     ALALLGGTPPYMKTPPLRFSESGAATHHTKGEQMDDTSPREVNRFTEEEVTRLIEDAR
                     AEERSRAEEMLAATRQMVERLQADARGAATARFRENLRRLGYAPALVEAPEMTALFEH
                     VARDSGTGEPETVKFGDTEATPAELFERLLERIAQHAARNGAFVRAGQEVPSAPHQRF
                     WEEHAPAETSVPAHVLDRASAASVERLNRAQKLAKEQGIPFRNALIRIMEG"
     gene            complement(3473..4627)
                     /locus_tag="PLT86_12650"
     CDS             complement(3473..4627)
                     /locus_tag="PLT86_12650"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HOT37503.1"
                     /translation="MGNPDEIIRRKGFAYVRDMERKNGFLYGVLRTRVQSVLSRRWSI
                     IPASTDARDLATAAFIREALDRIEGSFLDDLRSIAAAVRYGYSLAELVWQPWHSARFG
                     ERLTIRAIRDKPPEEFTFTSDEFGAVTGVQHHLAGRVRTFPLDRFVHFAFEPEGGNPY
                     GNGLLPRSFWHDWFMREGFKFWAVYLERFGSPLVRAVVPPATSPEERRQLEEVIESVQ
                     QRTGVVLPEGIALDFLEASRTGAAGFREFIEEQKQAIAIIVLGQTLTTFVGARGSLAL
                     GTVHQTTKEEIVRADAEALATVVNEQIIRRLVDANFAGVERYPVWAWEMTSPQDLEAL
                     ARVLGRLQAAGIRVPREWVERMFGVRLPETEAATAAPAGTANAQGHGSAA"
BASE COUNT          731 a         1598 c         1622 g          913 t
ORIGIN      
        1 atcctggcga accacgcgag ggcgatgtcg aacagctccc cggcgcgcac ccgtttcgtg
       61 tcgtcgccca gcccggccag cacgtcgtag acgaaacttg ccgccagccg gcaggtcgca
      121 aacgccacga gcggcggagt agccggggtg tcgtccgaat cagggaactt ctgcccgtcg
      181 tcccgcggca tgaagcgagg ccccaccagc gcatccgcca gcgcacttgc cgactggatg
      241 aacggcgccc gttcggcctc ggtgtcgagg gattcgggca gcgtagcggg tagttcgcgc
      301 agaagctccg cgtcggtggc gtagtgtgcc atcgttcctc ctttccgtga agggagccgg
      361 cccggattaa tccgccggat cggcccccgg tgtgaatcag tgcgtggctc aggcgattgc
      421 gttcttgatc aggtagcagc acgctgtgga cgcaatgacc tcggccgtct cctcccgcac
      481 acgcaccacc gtgcaggtct tgtacggctc gcgatacgtt tccaccaccc ggtcggtgaa
      541 gacaaaggtg tacgtggcgg cgggcgtgaa ccggctcggc atcgggggcg tgtagagcaa
      601 caccgcgttc ttgccccaga cgtatgccag cgccgaggcc tgtccctcgt tggaggtgcg
      661 ataccgcgcg ttgccgacga gcacccgctc cacgccgagc aggtcggcca tcatggcctc
      721 cgtcacggcg ccgctgcggg tgtatttgaa cgcctcgagg atatcggggt gccgcttcag
      781 ggtgtcgaac acctcccgcc ccatcaccag cgtgttcggg ttgggtgctc ccgccgcgtg
      841 gatggtgctc cgggcggtat ccacgttggc gatgggcgtg ctgttggtgt agtcgttcca
      901 ctggctggtg ccggaaagcg tggtgttctg cgtcacccgg ctcgtggagg tgaggatgtc
      961 ggcgagccgt ttttcgcggc acgtcatgat gatgccggtg gccttctcca cggcggcgat
     1021 ttcgatatcc gccccggcgt cggcgttgcg gcgctcgcgg tcgtcaacgg gcacctcgag
     1081 gccgtattcc tcgcacacga agctggccgt ggccggcgcg aactcggtgc gggcgaacag
     1141 gctttttggc gtgcgcccgg tggtgggcgt ctggaaggcc tccgttcccc agacgaagta
     1201 cttgtccgat tccttgtcca cccggatgcg cgggctcacg cggtcggcga tgaactcgtc
     1261 gttgtggaag tcgagactga tgtgcgaaag cgcggcgtcc acgtgaagat ccgccggctc
     1321 cgcgaaccga tccttagcca gcggacgtga ttgtaatgcc atgatgtctc ctttgtgtgt
     1381 ggttatgttg attgatcccg tgaataccag gtggggcagg gatgctgcct gtcagccgcc
     1441 gttgcaggtc gtctcgatac acctgccccc ctgccgggcg gctgccactc gacgaccgtt
     1501 gccgccatca cgcgcgggtc tccgagtggc gcggcggcat ttcgacaggc tggcccgcag
     1561 cacgctcaat gaccgccgcg ttgtatcgag gagcccgcgc gctggcccct ccgtgtgttg
     1621 tgccgtcatt cccgcgtgct cggccgagca cgaagcgcag gcgggaatcc acgccatact
     1681 cgttcccccg tggaagcggc gccaccggca tgccaaagtc gtcagttttc gttccaatca
     1741 cagcaattca ggctcattgt tctggattcc cgcctgcgcg ggaatgacgg taccgtgtgt
     1801 gcgaggtggc ccacagcaaa cccggaacat ttctcgtgcg cacgccgatg aacccctggt
     1861 ggggcaggca ttcgtgccgc ccgccgggcg gcctgcctgc cttatggcca gcctgtgtcc
     1921 gccgcggggc agacaggaat gtccgcccca ccaacatcgc tccccctacg cgttcttctg
     1981 gtaattggtc agcagcaccc ggatgatgtc gccgtcggcg ccggatgcct ccagcgcgat
     2041 tcccatcacg tagtgacccg ccgttgcggt gctgaacttc cccgcagtgg tggcggaaag
     2101 ctccacaccg gggttgatgg ctgccgccac aacgaccttg ctcacgccgc caacgcgcac
     2161 acgggccagt ttgccgtccg ccgagggcgc gttctgcagc accccgatgc accgctcccc
     2221 ggcgctgttg gcaagggcga tcttgcccgt ggtttccatc gtaacggcgt agaaccgctt
     2281 tgccgagagg tcggcccccg acacggtggt gatgtcctgc ccgccgaaat caactgccat
     2341 tctcagccct ccatgatgcg aataagtgcg ttgcgaaacg ggatgccctg ttcctttgcc
     2401 agcttctgtg cccggttcag gcgctccacc gacgcagcgc tggcgcggtc cagcacgtgc
     2461 gcgggaaccg aagtttcggc aggcgcatgt tcctcccaga accgctggtg cggcgccgag
     2521 ggaacctcct gccccgccct gacgaatgcc ccgttgcgcg cggcgtgctg tgcgatgcgt
     2581 tccagcaggc gctcgaacaa ctccgccggt gtggcttccg tgtcgccgaa tttcacggtt
     2641 tccggctcgc ctgtgccgga atcccgcgcc acgtgctcga acagcgctgt catttcgggt
     2701 gcttcgacca gcgcgggtgc gtaccccagg cggcggaggt tctcccggaa ccgtgccgtt
     2761 gccgccccgc gtgcgtcggc ctgcagccgc tcgaccatct gccgggttgc ggcaagcatc
     2821 tcctcggcgc gggagcgttc ctctgcccgg gcatcctcga tgagccgggt gacttcctct
     2881 tcggtgaagc ggttcacttc ccgcggggat gtgtcgtcca tctgttctcc tttcgtgtgg
     2941 tgcgttgccg cgccggattc ggaaaagcgg agcggcgggg ttttcatgta gggcggagtg
     3001 ccccccagaa gtgccagcgc ggtgatctgc gccgccctgc tgatgacttc aatcgagcgg
     3061 tacggccacc ggcccgcccg gatctggtcg tatacctccg tcgtgaggcc gacaaggtcc
     3121 gccacgaggt ggtttcccgc gatgcgcagg ccgtccatga agccgacagc gggtttttcg
     3181 ctctccgtgt cgctcgtgtg gccgatgatg acaggcggca ggtaaccgta tttgcgtttg
     3241 aggcgctgga acacctcaag cgcccggcgg gcccatgcgt cgtcataccg gaagccgcgg
     3301 tgctcgccca gtgcgaacac aggcacggcg cggatggtgt gcggcgcgcg tgtggtctgt
     3361 tggatgtggt acctcctttc tgtggacagg gtagaccggg tggtggggca ggctcgtggc
     3421 tgccctcggt gcagccccac aagggttgca gggtataggt catatggggg tcctatgcgg
     3481 ccgatccgtg gccctgcgcg tttgccgtgc cggccggtgc agccgtagcc gcttcggttt
     3541 ccggcaggcg cacgccgaac atgcgctcca cccattcccg cggcacacgg ataccggccg
     3601 cctgaagccg gccgagcaca cgggcgaggg cctccagatc ctgcgggctg gtcatttccc
     3661 acgcccagac ggggtagcgc tccacgccgg cgaagttggc gtccacgagg cggcggatga
     3721 tctgctcgtt caccacggtg gccagcgctt cggcatcggc gcggacgatt tcctctttcg
     3781 tcgtctggtg gacggtgccc agcgccagcg acccccgcgc gccgacgaac gtggtcaggg
     3841 tctgccccag cacgatgatg gcgatggcct gtttctgctc ctcgatgaac tcccggaagc
     3901 ccgccgcgcc ggtgcgggag gcctcaagga aatccagcgc gatcccctcc ggcagcacga
     3961 cgccggtgcg ctgctgaacg ctttcgatca cctcctcgag ctgcctgcgc tcctccggcg
     4021 aggtggcggg cggcaccacc gcgcgaacca gcggcgatcc gaaccgctcc agatagactg
     4081 cccagaactt gaacccctca cgcatgaacc agtcgtgcca gaacgagcgc ggcagcaggc
     4141 cgttgccgta cgggttgccg ccctccggct caaacgcgaa gtgcacgaac cggtcgagcg
     4201 gaaacgtgcg gacccggccc gccagatggt gctgcacgcc ggtgacggcg ccgaactcat
     4261 ccgacgtgaa cgtgaattcc tccggcggtt tgtcgcggat cgcgcggatg gtgagccgct
     4321 cgccaaaccg ggcgctgtgc cagggctgcc agacgagctc ggcgaggctg tagccgtatc
     4381 gcaccgcggc ggcaattgag cggaggtcgt cgaggaatga gccttcgatt ctgtccagcg
     4441 cttcacggat gaacgcggcc gtcgcgaggt cgcgcgcgtc ggtggaggcc ggaatgatgc
     4501 tccaccggcg gctcagaacg ctctgcacgc gggttcgcag cacgccgtag aggaagccgt
     4561 ttttgcgctc catatcgcgc acgtaggcaa atcctttccg gcggatgatt tcgtccgggt
     4621 tgcccatttc gggtttccag cgggaaagca ggcgggaagg cgctgcggat gcggtgtcag
     4681 gtagattcat gcggtatccc cggtgaagtg gtgcgaatgt gaacgtgtgc cccgcgacat
     4741 tgttgctgat gtggccccag cgtgagacgg atttcggagt ctgccccgtc acatactggc
     4801 tgaggcggcc tcagccgtga ggcggaatcc agagtctgcc ccgtgacgta ctggctgagg
     4861 caaa
//
LOCUS       DAOSOO010000226         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17576_length_4846_cov_3.829681, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000226 DAOSOO010000000
VERSION     DAOSOO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17576_length_4846_cov_3.829681"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLT86_12655"
     CDS             <1..320
                     /locus_tag="PLT86_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOT37504.1"
                     /translation="VAVLTRNHGAIYVNHTFKSHLSLAASFHVFAGESMFRYLEYPAA
                     ASDLAKRLVAAPLVPGSDGLVRLPRATGLGVELDMTTAKEYLQPVRIELGGKVLVEIT
                     DFD"
     gene            329..1093
                     /locus_tag="PLT86_12660"
     CDS             329..1093
                     /locus_tag="PLT86_12660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT37505.1"
                     /translation="MGTLDGKNAVVTGAGSGIGRSIAVRFAREGASVALLGRRKEMLE
                     ETLRAMPKGDHLVIPTDVTAAVQVAHAKDLIRDRWGTLHVLVNNAGVSAAAHPIASEL
                     AEWMLPIDTMFMGAVHCTRAFVPLMPVGSRIVMVTSIHRERVEKGASAYATAKGALTQ
                     YARALAVELADQGILVNAVAPGFVDTPMSVKAGGKNELAGEWFRTNYVEGHHLPLRRA
                     GKPDEIAGAVLFLAGPDATYITGETITVDGGLTITF"
     gene            1110..2132
                     /locus_tag="PLT86_12665"
     CDS             1110..2132
                     /locus_tag="PLT86_12665"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT37506.1"
                     /translation="MSKARIKGILTPNLVPLDRNGNIAEDELRRYVEWLVQKGVSGLY
                     PNGSTGEFTRFSPEERREIIRIVASQVNGRVPVLAGAAEATVRDTLAACAAYAEMGCT
                     AAAICAPYYFKLSQDSVGEYFAELARHTELDIVLYNIPQFANEISLDTIERLADFPRI
                     VGIKDSSRDFPRFLNLINRMRPRRPDFSILIGTEEIILPALLMGGDGGTIATSGIVPE
                     VVMKLYALAESGKIEEARKIQFKLLELINVMLFGAGFPEGFRAAASLRGFVMGQSRQP
                     LSPKEKCDLNAVRQTLHCILSEHGFVAEIEGAVGCEPLGQDASPSETEIQAIVAEVVR
                     QMRTNR"
     gene            2136..2765
                     /locus_tag="PLT86_12670"
     CDS             2136..2765
                     /locus_tag="PLT86_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT37507.1"
                     /translation="MSDTHEALTVVEVQGLPPAVVVADAMVKSAAITLIALEVNTLGA
                     LTIKASGDTAAVAAAHDAGKAIARQLDALVGATVIPRYSSPAYPMWIACKPSVSGLLK
                     SRNHLIPADGLLPVDDRAMGMLETRGFAGAVAALDAMLKSADVELLAKEKIGQTRVSV
                     LVRGDVSAVTASLAAGAVQAERVGTLASAHVIPHPDPVIQALFPRPQGQ"
     gene            complement(2707..3579)
                     /locus_tag="PLT86_12675"
     CDS             complement(2707..3579)
                     /locus_tag="PLT86_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37508.1"
                     /translation="MQVRVEDLEAYRDRLWCRLPSLRIHDEDEAVAYLQRIPMCLAFG
                     GYALGVPTMWTGACGLRKPVFPKHSHHDPAVGLIWRAKETLVHSGAAYYGRLFLNKPS
                     FVARSWLATVLAGYPRVKLSQEAQAVSETLEESGPLPTRELGVRSGIRDRRVLTKALE
                     EAQTALRVTKVEERSEPFTYVWGTLSTLYAQEIDQAKGIPQDVARRKIAALWVNSLGL
                     TQAGRLARILGWDERITRNALADLVTSGTIVDGVSLEGARGVWVGARSLLQEGITGPA
                     VEGKGLESQGRDAG"
     gene            complement(3587..>4846)
                     /locus_tag="PLT86_12680"
     CDS             complement(3587..>4846)
                     /locus_tag="PLT86_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT37509.1"
                     /translation="ESENVEATVIGEFTDTGRLVLRYHGVQVADLDMRFLHKGVPQVR
                     RNAVWRPPEERDPVFEEPASLNADLLALLSHPTIASKEWIIRQYDHEVQGGSALKPLV
                     GAENDGPSDATVIRPVLDSEKGVLLANGINPRYSLLDPYFMALAVIDEAIRQVVAVGG
                     NRSRTALLDNFCWGNPENPERLGELVRAARGCYEAAKAFETPFISGKDSFYNEYAVPS
                     GTIAIPPTLLISAICVIDDVQKLVSMDLKEPGHFVYVVGLTRAEMGGSHYWMLHDAVG
                     RDVPKVDLGTAPTAMDKMSAAIRSGCVRACHDCSEGGIGVAAAEMAFSGGVGCEIDLG
                     HLPVDSKHGRSDILLFSESPTRWICEVPPDRQKEFETIMEGTPFGRVGQTTASRRMRV
                     TGLSGDAVIDLSLDEMKEAWQRTLREV"
BASE COUNT         1015 a         1543 c         1343 g          945 t
ORIGIN      
        1 aggtcgcggt acttaccaga aatcacggtg ccatctatgt aaaccatacc ttcaaatccc
       61 atctttcgct cgcagcatcg ttccatgttt tcgcaggcga gtccatgttc aggtatctcg
      121 agtaccctgc tgcagcatca gaccttgcaa agcgcctcgt tgccgcgccg ttggttccgg
      181 gttcggacgg gctggttcgt ctgccgcgag ccacgggact cggcgttgaa ctggatatga
      241 cgactgcgaa ggaatacctg caaccggtgc ggatcgaact gggcgggaaa gtgttggtgg
      301 aaatcaccga tttcgattga ggaacgagat gggcacattg gacggcaaaa acgccgtggt
      361 tacgggggcg ggaagcggga ttggacgatc catcgccgtg cgttttgcac gcgaaggagc
      421 gtctgtcgcg ctactggggc gacggaaaga aatgctcgag gaaaccctcc gtgccatgcc
      481 gaaaggcgac catcttgtga ttccgaccga cgtaactgcc gcagttcagg tagcgcatgc
      541 aaaagacctg atccgtgatc gctggggtac tctccacgtg ttggtgaaca acgcaggcgt
      601 atcagcggca gcccacccca tagcctctga actcgcggaa tggatgctcc ccatcgatac
      661 catgttcatg ggagctgtgc attgcacgcg cgcgttcgtc cccctgatgc ctgtcggaag
      721 caggatcgtg atggttacaa gtatccatcg cgaacgcgtc gagaaaggcg cctccgccta
      781 cgcaacagcg aaaggcgcgc ttacgcagta cgctcgggcc cttgccgtag aactcgcgga
      841 ccaaggcatc ctcgtaaatg cagttgcacc cggctttgtc gataccccga tgtccgtcaa
      901 agccggcggg aaaaacgagc tcgccgggga atggtttcgt accaattacg tcgagggaca
      961 ccatctccct ctccgtcgtg ctggcaaacc ggacgaaatc gctggcgcgg tgctattcct
     1021 cgcgggcccc gacgcgacct acattactgg agaaacaatc accgtcgacg gcggattgac
     1081 catcaccttc tgaagaaaag tggaatgcaa tgtcaaaggc cagaatcaag ggaatcctca
     1141 cgccgaatct cgtgccactg gatcgtaacg ggaatatcgc ggaagacgag ctgcgtcgtt
     1201 acgtagaatg gctcgttcag aagggcgtga gcggcctcta ccccaacgga agcaccggag
     1261 aattcacccg tttcagtccg gaagaacggc gcgaaatcat ccggattgtc gccagccagg
     1321 tgaatggccg ggttcccgtc ctcgccggcg cagccgaagc aaccgtccgt gatacactgg
     1381 ctgcctgcgc cgcgtatgcc gaaatgggtt gcactgcggc agccatttgc gcgccttact
     1441 atttcaaact ctcccaggat tccgtaggcg aatacttcgc cgaactcgcc cgccacacgg
     1501 aactcgacat cgtgctttac aacatcccgc agtttgccaa cgaaatcagc ctggacacca
     1561 tcgaacgcct tgctgatttc ccccgcatcg tcgggatcaa agactccagc cgggattttc
     1621 cgcgattcct taacctgatc aaccgtatgc ggccgcgccg acccgatttc agcattctca
     1681 tcggtaccga agaaatcata ctgcccgcgt tgttgatggg tggggatggg ggaacaatcg
     1741 ccacaagcgg aatcgttccc gaagttgtga tgaagttata cgcgcttgca gaatccggta
     1801 agatcgagga ggcgcgcaaa atccagttca agctgctgga gctcatcaat gtcatgctgt
     1861 tcggggcggg attccctgaa ggcttccgtg cggccgccag cttgcggggc ttcgtcatgg
     1921 ggcagtcccg ccagccgctc tccccgaaag aaaaatgcga tctcaacgcg gtgcgccaga
     1981 cccttcactg catcctcagc gaacacggct tcgtcgcgga aatcgaagga gccgttgggt
     2041 gcgaacccct tggacaggac gcctcccctt ccgaaacaga aattcaggcc attgtcgcgg
     2101 aggtcgtgcg tcagatgcgg acgaaccggt aacacatgag cgacacgcac gaagctctga
     2161 ccgtcgtgga agtccaaggc cttccgcccg cagtggtcgt ggcagacgcg atggtgaagt
     2221 cggcggcgat cactctcatc gcgctcgagg tcaacacgct tggtgccttg accatcaaag
     2281 cgtcggggga tacggcagcg gtcgcggccg cgcatgacgc gggaaaagca atagcccggc
     2341 agctggatgc tctggtcggc gcgacagtaa tcccgcgcta ctcgtcgccc gcatatccca
     2401 tgtggatagc gtgcaaaccc tcggtcagcg gcctcctgaa atcccgaaac cacctgatcc
     2461 ccgcagatgg gctccttccg gtggatgacc gtgcaatggg gatgctcgaa acgcgtgggt
     2521 tcgccggcgc ggtggcggca cttgatgcaa tgctcaaatc cgcagatgtg gagcttctcg
     2581 ccaaagagaa gatcgggcag acaagggttt ccgttctcgt caggggcgac gtttcggctg
     2641 tcacagcgtc tctcgctgcc ggggccgtgc aggcagaacg agtcggcacc ctggcttcag
     2701 cgcatgttat cccgcatccc gaccctgtga ttcaagccct tttccctcga ccgcagggcc
     2761 agtaatccct tcttgaagca gactacgggc gcccacccag accccccgcg cgccttccag
     2821 cgagacgccg tccacaatgg ttccggaagt gacgagatcg gcaagtgcgt tccgggtaat
     2881 gcgctcatcc catccgagaa tccttgcgag acgccccgcc tgcgtcaatc caagcgaatt
     2941 cacccacaac gcagcgatct tcctccgcgc gacatcctgc ggaatcccct tcgcctgatc
     3001 aatttcctgc gcatagagcg tgctcagcgt gccccacacg tacgtgaacg gctcactccg
     3061 ttcctccacc tttgtaaccc gcaacgccgt ctgggcctcc tcaagtgcct ttgtaaggac
     3121 ccttcgatca cgaatccccg accgaacgcc cagttctcgt gtgggaagag gaccggattc
     3181 ttccagcgtt tccgaaactg cctgtgcttc ttgtgaaagc ttcacccgcg ggtaccctgc
     3241 cagcaccgtg gcaagccagc tcctggcaac gaacgagggt ttgttgagaa agagacgccc
     3301 gtagtacgcc gcaccagaat ggaccagcgt ctcttttgca cgccagatca atcccactgc
     3361 cgggtcgtga tgggaatgct tggggaaaac tggtttgcgg aggccacatg cgcctgtcca
     3421 cattgtgggc acaccgagcg catacccgcc gaaagcgagg cacatcggaa tgcgctgcag
     3481 atacgcgacg gcctcatcct catcatggat tcggagcgat ggaaggcggc accataaccg
     3541 gtcccggtac gcttccagat cttccacgcg gacctgcatc cggagttcac acctctcgca
     3601 aggtgcgctg ccaggcctct ttcatctcgt ccagcgacag gtcaatcacc gcgtcgccgg
     3661 agagtccggt gacacgcatc ctccgcgaag ccgtcgtctg cccgacacga ccaaatggag
     3721 tgccttccat aatcgtctcg aactctttct gccggtcagg aggcacctcg catatccacc
     3781 tcgtgggcga ctcactgaac aacaggatat ccgatcgccc gtgctttgaa tccacgggca
     3841 agtgccccag atcgatttca cagcccacgc cgcccgagaa cgccatctcg gccgccgcca
     3901 caccgatacc tccctcagag cagtcgtggc atgcccgcac gcatcccgac cgtattgcgg
     3961 cgctcatctt gtccatggct gtcggcgcgg tcccgaggtc aaccttcggc acgtcacggc
     4021 cgaccgcatc atgcagcatc cagtaatgcg aaccacccat ctccgcacgc gtgagcccga
     4081 ccacgtacac gaaatggccg ggttccttca ggtccatgct taccagtttt tgtacgtcgt
     4141 caatcacaca tattgcgctg atcaacagcg tgggcggtat cgcaatcgtg ccggaaggaa
     4201 cggcgtactc gttgtagaaa ctgtccttgc cgcttatgaa aggcgtctcg aatgccttcg
     4261 ccgcctcata acatccccgc gcagcccgta ccagttcccc gaggcgctcc gggttctccg
     4321 gattgcccca gcagaaattg tccagaagcg ctgttctgct gcggttgccc ccgaccgcca
     4381 caacctgccg gatcgcctcg tcaataacgg caagcgccat gaaatatgga tccaggagtg
     4441 agtaccgcgg gttgataccg ttggcaagga gcacaccttt ttcgctgtcg agaaccggac
     4501 ggatcaccgt tgcgtccgaa gggccgtcgt tctccgcgcc aaccagcggc ttcagtgccg
     4561 acccgccttg cacctcgtga tcgtactgcc gaatgatcca ctccttactt gcgattgtcg
     4621 gatgcgaaag aagcgccagc agatccgcat tcagcgacgc gggttcttca aaaaccggat
     4681 cgcgctcctc cggcggtcgc cacacagcgt tcctgcgaac ctgaggcacg cctttgtgca
     4741 gaaacctcat atccaggtcc gcaacctgaa caccgtggta ccgaagaacc agcctcccgg
     4801 tgtccgtaaa ttctccgatc accgtcgctt caacgttttc ggactc
//
LOCUS       DAOSOO010000227         4820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17690_length_4820_cov_4.583631, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000227 DAOSOO010000000
VERSION     DAOSOO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17690_length_4820_cov_4.583631"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            982..2160
                     /locus_tag="PLT86_12685"
     CDS             982..2160
                     /locus_tag="PLT86_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37510.1"
                     /translation="MHRPLSDFGVTPGGDPVKNAARLQEGIDWASPRGGALYVEPTDE
                     PYRIAGGVILRQNVSLIGVHGPVGRGTRHPDRAQPVGSVFAIEDETSPFLTVEHATQV
                     RGIQFWYPRQTADDASRIIPYPATIRVSPDTGAQGVTLSCLTFFGEYLAMDFAASPKC
                     ICEQILFEHCYGYPLGGTFIRIDYCYDIPRILHCHVNPANRRFIDGGHGKAVIDATMA
                     RKTFCYSIDHTDNAQLMDLFTFGTWGGIHLGPATYGQLTNFNLDCVAVGILKQGDQNF
                     NRNWQIAQGSIIANGGAPLEDIHPLIVEGQGHTALANVESFSGHNGALTTLGQSQDFL
                     LLRGNERLTVSMFGCRMRNYAAAEPVTVQNPKAVVQAVACVDHHENPWPAKPFCARGE
                     "
     assembly_gap    2842..2941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2950..>4820
                     /locus_tag="PLT86_12690"
     CDS             2950..>4820
                     /locus_tag="PLT86_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37511.1"
                     /translation="MNGTGYTPQRTGTTIYVSRLGDNSDGRSWTTAFTTVQAGLDAIP
                     DNRGGHRVVVRPDTYMEANLHPAFPGAEGSYNLFDVDFDGSLGSGAAGYAVLDASDPK
                     KGMQSIDYWQVPRSSVEYPGVEWDRWIVRHVYATGGDAGLFWDNDTTPFSVIVEDSVG
                     IGRAFGGGAGNVLPREGEPMIWRRCCLWSLDWWGDTAGAYCRAENPGPREEPDFIFED
                     CTLVGPQCALKSGNPGFSTYSHIRVERCRLIVLNFSQPRGTPSDGIIQSVIDGKYLHV
                     DLEDTTLMGYKVFGVRQNKETVDQIGYTTKGCVQAYVQFEQEVPKGIQPMGHWPADVF
                     EFIKPPSPAAPAVLSARRPVLRDPESVENHVCELTPFVWKGRLSHMTCVRPVDADPDR
                     GLYLRLSDAETGAELARFAEGYSLASAIVWNDTFYAFASRHGDGTWNDVTLFKSSDLR
                     NWTQKVVIEQEGAEHLFNTSVCATPGGFVMAYESDDPAYVPFTVKYAESADLENWTKV
                     SDAVFGPDRYAACPCVRYSDGWFYQMYLEHRTPRWFFETHIARSKDLKTWHLSPMNPV
                     ITPEGLDEGNNASDPEIVEFAGKTYLYYAVGDQLTWTRLKRKTYDGPMARFFAAWWAG
                     "
BASE COUNT          874 a         1493 c         1561 g          792 t
ORIGIN      
        1 ggagggaccc gctttgtcgg gtccgcaaca gggaagtggg cagaaaggcg gcgcgggaaa
       61 ccattaatgg cgtcctgcat ccgccacccg gcccagcgaa ccgggctacg gaatttcgca
      121 ccgttggcgc tgccgccgcg ccctggggaa ggcctgaagg gccgagatag atgagcccgg
      181 ggcagcgtcc cgggcgtggg gaggcgccga cctcagggct ggtgggtccc gcgccgaacg
      241 cgacgttccc atcgtgacgc tgcgtgttgg taattttgga ttcccgcctg cgcgtcgtgc
      301 tcggttgagc acgcgggaat gacggtgtgt gggaatgccc gatggaatac gcccggccca
      361 gcgagccggg ctacggtatt tcgcaccgtt ggcgcttgct gcgtggggag tgcggttggt
      421 gaggcctgca gggccgcgat accttagccc ggggcaacgt cccgtacgta gggaggcgcc
      481 cacctcaggg ctggtgggtc acgcgacgtt cgcatcgtga cgttgcgtat cggtgccttg
      541 gattcccgcc tgcgcctcgt gctcgccgga gcacgcggga atgacgacac aggcgcatgc
      601 tggaaacaac acgcccggcc ctgcgagccg ggctacggaa tttcgccccg ttggcgctgc
      661 cgggaggcga gggcggttgg ggtgaggccc gaagggccgc gatagctcag cccggggcag
      721 cgtcccgggc gtaggaaaac atggccgaga cctcccttcc gccggcgtgt ccctgtaccg
      781 gcgggtggtt ctcggatcgt tgagagaaat gctgcgtagc acgggcatct tgcccgtgca
      841 cggtataacg ccgctttgcg ttctggttcg ggcaagatgc ccgcgctacg gctggaatga
      901 ggctaggtta ccggcgcgga ttgatgcaac aaggaatctc cgcccgcgta tggctccccg
      961 caatcaccga aaggaacaac cgtgcatcgc ccactgagcg acttcggcgt cacccccggt
     1021 ggtgacccgg tcaaaaacgc cgcgaggctg caagaaggca ttgactgggc atccccccgc
     1081 ggcggcgcgc tgtacgtgga accgacggac gaaccgtacc gcatcgccgg aggggtaatt
     1141 ctccggcaga acgtgtccct catcggtgtg cacggcccgg tggggcgagg aacgcggcac
     1201 ccggacaggg cgcagccggt cggtagcgtg ttcgctatcg aggacgaaac cagcccgttt
     1261 ctcaccgtcg agcacgcgac gcaggtgcgc ggcatccagt tctggtatcc ccgtcaaaca
     1321 gccgacgacg ccagccgcat catcccctac cccgccacca tccgcgtctc gccggacacc
     1381 ggcgcgcagg gggtgacgct ctcctgcctc acgttcttcg gcgagtatct ggcgatggat
     1441 ttcgctgcca gcccgaaatg catctgcgag cagatcctgt tcgagcactg ctacggctac
     1501 ccgctgggcg gcacgttcat ccgcatcgac tactgctacg acatcccgcg tattctgcac
     1561 tgtcacgtca accccgcgaa ccgccggttc atcgacggcg gccacggcaa ggcggtgatc
     1621 gacgcaacca tggcgcggaa aacgttctgc tactccatcg accacacaga caacgcccag
     1681 ctcatggacc tgttcacgtt cggaacgtgg ggtggcattc accttggccc ggcaacctac
     1741 ggtcagctca cgaattttaa cctggactgc gtggcggtgg gcattctcaa gcagggagat
     1801 cagaacttca accgcaactg gcaaattgcg cagggctcca tcatcgcaaa cggaggcgcg
     1861 ccgctggagg acatccatcc gctcatcgtg gagggacagg ggcacaccgc gctcgcgaac
     1921 gtcgagtcat tctccggcca caacggcgcg ctgacgaccc tcgggcagtc gcaggatttc
     1981 ctgctcctgc ggggcaacga acggctgacg gtgagcatgt tcggctgccg catgcgcaac
     2041 tacgccgccg cggagccggt gaccgtgcaa aacccgaagg cggtcgttca ggcggttgcc
     2101 tgcgtggatc accatgagaa cccctggccg gcgaagccat tttgtgcacg cggggagtga
     2161 tgcgccacgg cgcgttcggc gaacgcaccc tgcctttttg gagggacccg ctttgtcggg
     2221 tcccagacac ggcggcggca gcccgcccca ccggtcaaag cgcggcgcat ttcgtgcccg
     2281 gccctgcgag ccgggctgag atatttcgcg tcgacggcgc cggtgcgtct cgatacagcg
     2341 aaccggtcgc caaacttcgg gaaccgcagg aggcctgttg aagcgccggt ttgtttctca
     2401 gcgaccggtt cgccactcga cgaccggggt cgttgcacga ctaccagcac tcactccggt
     2461 agtcattccg cgcgctcaat cgagcacgac gcgaaggcgg gaatccagaa gaggcatctg
     2521 cggcggtctc ggcgagaccg ccctaccctg caaaccgggc gacgggggca aaaggccccg
     2581 aatgggcggg tccctgagcc tgtcgaaggg cacgcccggg aaatgcgttg gcgggagcac
     2641 gctcagggcc cgcgcaggat gggcgatcgc tggtgcaagc gcctggggaa ggcctgaagg
     2701 gccgcgatag tttagcccgg ggcagcgtcc cgggcactac agcgaaaata ccgggtcata
     2761 gctccgggaa agggaaggca gaatgacatc gagcatgaac ggaacgggat acacccctca
     2821 acgcacaggc acgaccatct annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 ncatcgagca tgaacggaac gggatacacc cctcaacgca caggcacgac catctacgtc
     3001 tcccggctcg gggataactc cgacgggcga tcgtggacca ccgcattcac cacggtgcag
     3061 gccggtctgg acgccatacc tgacaatcgg ggcgggcatc gagttgtcgt ccggcccgac
     3121 acgtacatgg aggcgaatct gcaccctgcc ttcccgggag cggagggttc ctacaacctt
     3181 ttcgacgtgg acttcgacgg gtctctgggc tccggcgcgg caggatacgc ggtgctggac
     3241 gccagcgatc caaaaaaggg catgcagagt attgattact ggcaggtgcc gcgcagctcc
     3301 gtggaatacc ccggcgtgga gtgggatcgc tggatcgtcc gccacgtcta cgcgacgggg
     3361 ggcgatgcgg gcctattctg ggataacgac acgactccgt tctccgtcat cgtggaagac
     3421 agcgtcggca tcggcagggc gttcggcggc ggcgcgggca atgtgctccc ccgtgagggc
     3481 gagccgatga tctggcggcg gtgctgcctc tggtcgctgg actggtgggg cgacaccgcc
     3541 ggggcgtact gccgcgcgga gaaccccggc ccgcgcgagg agccggattt catcttcgag
     3601 gattgcacgc tcgtcgggcc gcagtgcgcg ctgaaatcgg gaaaccccgg tttcagcacc
     3661 tattcgcaca tcagggtcga gcgctgccgc ctcatcgtgc tgaatttctc gcagccgcgc
     3721 ggcaccccgt ccgacggcat catccagagc gtgatcgacg ggaaatacct ccacgtggat
     3781 ctggaagaca ccaccctgat gggctacaag gtgttcggcg tgcgccagaa caaggagacc
     3841 gttgaccaga tcggctacac cacgaagggt tgcgtgcagg cgtatgtcca gttcgagcag
     3901 gaagtgccga agggcattca acccatgggc cactggcctg cggacgtgtt cgagttcatc
     3961 aaaccgccat ccccggcggc tcccgccgtc ctgtcagccc gtcgcccggt tctccgagac
     4021 ccggaatccg tggaaaacca tgtctgcgag ctgacccctt ttgtgtggaa ggggcggctc
     4081 agccacatga cctgcgtgcg cccggtggat gccgatcccg accgggggct ctatctccgg
     4141 ctctcggatg cggaaacggg cgccgaactc gcacgcttcg ccgagggata cagcctcgcg
     4201 tcggcgatcg tctggaacga caccttctac gcgtttgcct cccggcacgg cgacggcaca
     4261 tggaacgacg tcaccctgtt caagtcgtcc gacctgagga actggacgca aaaggtcgtg
     4321 atcgagcagg agggcgcgga gcatctgttc aacacctccg tctgcgcaac gccgggcggg
     4381 tttgtgatgg cgtacgaatc cgacgatcct gcgtacgttc cgttcacggt gaaatacgcc
     4441 gaatcagccg accttgagaa ctggacgaag gtgtcggatg ccgtgttcgg gcccgaccgc
     4501 tatgccgcat gcccgtgtgt ccggtattca gatgggtggt tctaccagat gtatctggag
     4561 cacagaacgc cgcgctggtt tttcgagacg cacattgcac ggtcgaaaga cctcaaaacg
     4621 tggcatctga gcccgatgaa tcccgtgatc accccggagg gtctcgacga gggcaacaac
     4681 gcgtcagatc cagaaattgt ggagtttgcg gggaaaacct acctctacta cgccgtcggc
     4741 gaccagctca cgtggacgag gctcaagcgc aagacgtatg acggccccat ggcgcgtttc
     4801 tttgcggcgt ggtgggcggg
//
LOCUS       DAOSOO010000228         4808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17740_length_4808_cov_5.043551, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000228 DAOSOO010000000
VERSION     DAOSOO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17740_length_4808_cov_5.043551"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLT86_12695"
     CDS             <1..320
                     /locus_tag="PLT86_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37512.1"
                     /translation="VAAEWGSIYAWSHPAVRIVTRGWRVVSPDVIEVDTKTMMWAGSG
                     PEMEPSDMIRVPWDSPCDLKMTWRRQPAVPQARNGWRILKTEPAFVRMESTVPFRWCY
                     QGW"
     gene            472..1797
                     /locus_tag="PLT86_12700"
     CDS             472..1797
                     /locus_tag="PLT86_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT37513.1"
                     /translation="MPAKRDPQALCAYLADRVFDGRRVVPHQAVLVENRRVVCVLPAR
                     ELTAGTPIAAYPGCTILPGLVDIHTHFMRWQGPLYLAHGVTTVRDTGNPLNWILARRS
                     EWEKREWPRMFCTGPLIDGPSPVHPVTSRPCTNISDAIRGVREVAAAGVDGIKLYVGL
                     ETGWIPAMVREANAAGLPVSMHALQTGMVLPARAGVQEFFHLDGILHDVWPDHPPGWL
                     DAWGLPGFEQTLDRQKYLADEIAEREIVMTPTLAYWDSQWRGRLRAYKRYPALRDVPR
                     EVINWQVPGEPDREGSARWKRALKAAQRFVALLREREVTVLAGTDTPCGAAPPGLSLW
                     REMRLLVEAGMNTREALTAATSATGKIPGFQRVGTLCREAAADLVVVRGNPLDAIPDP
                     PDIVLVVRNGEEFDPAALKSRAETVDLRDEPWARQFQAHAEHTPATPWE"
     gene            1824..3890
                     /locus_tag="PLT86_12705"
     CDS             1824..3890
                     /locus_tag="PLT86_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37514.1"
                     /translation="MAAINEKSIELTHGAAKLVVDARTGSVLRLEDTRTGVARIDAKK
                     DGRNDGCLFRTMVPAENWWSRVADSSHQKRVRVSQRGGGATVEFPALVAADGAETGVS
                     ARVDIRPGGPDEFVFVLHLENSGSSTVLDTAFPVLGGWHERGTGRIALGANSFVDPGC
                     VARGAGNNYARNGRRWAWQYPVSIACPWVDFPGSGGGLSYINYMAEARNGRFWIENMD
                     GYGDDFRLALGWIHLIALPPGQSWTSPPVGVSVHNGDWRAAADRYAAWFDTQHPPDYS
                     RPAIRSRIGFQNVFFRGFDGTPIRKLDRIPHVAAVGRKFGVDMLCVWDTLTLGNYARH
                     NPRDLTDYPPDERQLIARGLQRAEREGTSTCALINFRHPNVGLHLPDKRLLDRVQKRY
                     TGTPRTENWAANHTMGDLWSHHIGPQSYVFSPFSRFQRDRVIRISRDYLALGYTSMFY
                     DQPFEYDPDYGFAGRGKQPDMAHHEALTLVGKVRRLLLAGNPNAVIIGEESDILATPV
                     IDQWMSWSISEPSPALIERMELMRFSIPHTILSWVVDHEPERASLAFAFGMQLCLMVH
                     GGDATLEDEPAFGSHVHALANLRKATAERTVMARFRGTRGVEVEGGEGFVAYAYDSPA
                     GPAIIAAAAGKKAKGKVKVDRDAFSASGTPRGGTLFRLDGSTVACSGNTRVFVLGANE
                     VAVWVL"
     gene            3917..>4808
                     /locus_tag="PLT86_12710"
     CDS             3917..>4808
                     /locus_tag="PLT86_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT37515.1"
                     /translation="MKIVHMAGKSVVKITDAPEPVPGPGEVVIETAMSGICGSEMHTY
                     REEGMETGNGGHEAVGTVVKLGECVPNLKVGQRVGASAIAGCGACPACAKGQYTWCPN
                     NRFYGNMHAERFLAAANACHILPDDVPWDAGLLLTGDGLGVPYHTSRKIASRDITTVA
                     VFGVGPIGLGNVLMQAHLGRRVIAVDISPTHLEHARNCGAAEVVNPKDSDPVEAVKAL
                     TGGKGADVCMECAGRQETAMACFSAVRTAGTVVFNGEQGPLPLSPSSHFIRRDITAVG
                     SWFYHFSEFGAMLELYRNGLD"
BASE COUNT          853 a         1551 c         1631 g          773 t
ORIGIN      
        1 gcgtggcggc ggaatggggt tccatctacg cgtggagcca tccggcggtg cgcatcgtca
       61 caaggggctg gcgcgtcgtt tcaccggacg tgatcgaggt agacacgaaa accatgatgt
      121 gggcgggcag cggcccggag atggagccca gcgacatgat ccgcgtcccg tgggatagcc
      181 cctgcgacct gaagatgacg tggcgcagac aacctgctgt gccgcaggcc cgcaacggct
      241 ggcgcattct gaagacggag cccgcgttcg tccgtatgga gagcacggtc ccgttccgct
      301 ggtgctatca ggggtggtag tgccgcccgt cagggcggcg caccctgacg gctggctggc
      361 ggtttgcctc gccgcgcgcg ggatactagc ttctgacggc aggcaccgtg ccgctggcgc
      421 aggatgctcg ccccgggagt tccccggtcc ccacggaggc gtacgacttg tatgcccgcg
      481 aaacgagacc ctcaggcgct ctgcgcgtac ctcgccgacc gcgtgtttga cggccggcgt
      541 gtcgtccccc atcaggccgt gctggtggag aaccggcgcg tcgtatgcgt tcttcccgcg
      601 cgggaactca ctgccggaac acctattgcc gcgtaccccg ggtgcaccat cctgccggga
      661 ctggtggaca ttcacaccca cttcatgcgg tggcaggggc cgctctacct cgcgcacggc
      721 gtgacaaccg tccgggatac cgggaacccg ctgaactgga tactcgctcg ccgatcggag
      781 tgggaaaagc gggaatggcc ccgcatgttc tgcaccggcc cgctcattga cggcccaagc
      841 ccggttcatc cggtcacctc gcgcccgtgc acgaacatct ctgacgcgat acgcggtgtt
      901 cgcgaggtcg ccgccgccgg cgtggacggc atcaagctgt acgtcgggct ggagaccggc
      961 tggataccgg ccatggtgcg cgaagcaaat gccgccggtt tgccggtgag catgcatgcg
     1021 ctgcagacgg gaatggtcct ccccgcccgg gccggtgtgc aggaattctt ccatctcgac
     1081 gggattctcc acgacgtctg gcccgaccac ccgcccggct ggctggacgc atggggcctg
     1141 ccggggttcg aacaaacact tgaccggcag aaatacctcg ccgatgagat cgccgagcgg
     1201 gagatcgtga tgacgccgac gctggcgtac tgggattccc agtggcgggg gcgtctccgg
     1261 gcttacaaac ggtaccccgc gttgcgtgat gtcccccggg aagtcatcaa ctggcaggtt
     1321 ccgggagagc ccgacaggga aggttcggcg cgctggaaac gggcattgaa ggccgctcag
     1381 cggttcgttg ctcttctgcg ggagcgggaa gtgacggtgc ttgccggaac cgataccccc
     1441 tgcggcgcag ctccgccggg gctgagcctc tggcgcgaga tgcggctgct ggtggaagcc
     1501 ggaatgaaca cgcgtgaggc gctgacggcc gcgacatcgg caacgggcaa gatcccaggg
     1561 ttccagcggg tgggcaccct gtgcagagag gcggcagcgg atcttgtcgt ggtacggggt
     1621 aacccgctgg acgcaatacc cgacccgccg gacattgtgc tggtggtaag aaatggcgaa
     1681 gagttcgatc cggccgccct gaaatctcga gcggaaacgg tcgatctgag agatgaaccg
     1741 tgggcaaggc agttccaggc gcatgcggaa cacacccctg ccacgccgtg ggagtaacca
     1801 ctgacgggaa aaaggagcta ccgatggcgg ctatcaacga aaagtcgatc gaactcacgc
     1861 acggcgcggc gaaactggtc gtcgatgcgc gcacggggtc tgtgttgagg ctggaagaca
     1921 cgcgcaccgg tgttgcccgc attgacgcga agaaagacgg ccggaacgac gggtgcctgt
     1981 ttcgaactat ggttccggcg gagaactggt ggtcgcgcgt ggcggatagc tcccatcaga
     2041 agcgcgtgcg ggtttctcag cgcggcggcg gcgccaccgt cgagttcccc gcactggttg
     2101 ccgcggacgg cgcagaaacc ggcgtatctg cgagggtgga tatccggccc ggcggaccag
     2161 atgagttcgt gttcgtgctc catctggaga actccggctc ctccacggtg ctcgacaccg
     2221 catttcccgt tcttggtggc tggcacgagc ggggaacggg gcggattgcc ctgggcgcga
     2281 acagcttcgt ggaccccggt tgtgtggccc gtggcgccgg gaacaactac gcccgcaacg
     2341 gcagacgctg ggcgtggcag tatccggtca gcatcgcctg cccgtgggtg gacttcccgg
     2401 gatcaggcgg cggcctgtcg tacatcaact acatggccga agcgcgcaac ggccgcttct
     2461 ggatcgagaa catggacggt tacggcgatg acttccgcct cgcgctgggg tggattcacc
     2521 tcatcgcgct cccaccgggg caatcgtgga cgtcaccccc cgtgggcgtc tccgtccaca
     2581 acggcgattg gcgggccgcc gccgaccggt acgcggcctg gttcgacaca cagcacccgc
     2641 cggactactc ccgcccggcc attcgatccc gcatcggctt ccagaacgtg ttcttccgtg
     2701 ggttcgacgg cacacccatc cggaaactcg accggatacc gcacgttgcc gccgtgggcc
     2761 gcaaattcgg cgtggacatg ctgtgcgtgt gggataccct caccctcggc aactacgcac
     2821 ggcacaaccc ccgcgacctc accgattacc cgccggacga acgccagctc atcgcccgtg
     2881 gcctccagcg ggcggagcgg gagggcacca gcacctgcgc gctcatcaac ttccgccacc
     2941 cgaacgtcgg cctgcatctg ccggacaaaa ggcttctcga ccgcgtgcag aagcgctaca
     3001 cgggaacccc gcgcaccgaa aactgggcgg ccaaccacac catgggcgat ctctggtcgc
     3061 accatatcgg gccgcagagt tacgtcttca gcccgttttc ccggttccag cgggaccgcg
     3121 tcattcggat ttcgcgcgat tacctcgccc tcggctacac ctccatgttc tacgaccagc
     3181 cgttcgagta cgacccggac tacggattcg ccggcagagg gaaacagccg gacatggcgc
     3241 accacgaggc gctgacgctc gtcggaaagg tgcgccggct gttgctcgcg gggaacccga
     3301 acgcagtgat aatcggcgag gagagcgaca tcctggccac gccggtcatc gaccagtgga
     3361 tgagctggag catctcggag ccgtcgcccg cgctcatcga gcggatggag ctcatgcgtt
     3421 tctcgatacc gcacacgatc ctctcgtggg tggtggatca cgagcccgaa cgcgcgagcc
     3481 ttgccttcgc gttcggcatg cagctctgcc ttatggtgca cggcggggac gccacgctgg
     3541 aggacgagcc cgccttcggg agccacgtcc acgcgctggc gaacctgcgg aaggcaacgg
     3601 cagagcgcac cgtcatggcg cggttccggg gcacgcgggg cgtcgaggtg gagggcggcg
     3661 aagggttcgt cgcgtacgcg tatgacagcc ccgcgggacc ggccatcatc gccgcagcgg
     3721 cgggaaagaa ggcgaaagga aaagtgaagg tggaccgcga cgccttctcg gcttccggca
     3781 cgccacgcgg cggtaccctc ttccgcctcg acggcagcac cgttgcgtgc agcggcaata
     3841 cgcgcgtatt tgttctgggg gcaaacgagg tggcggtgtg ggtgttgtaa ggcgcgggca
     3901 tgaaggagac gacaggatga aaatcgttca catggcgggc aagagcgtcg tgaagatcac
     3961 cgatgcgccg gaaccggtgc ccgggccggg cgaggtagtc attgaaaccg caatgtcagg
     4021 catctgcggc agcgaaatgc acacctaccg tgaagaaggc atggaaacag gcaacggcgg
     4081 acacgaggcc gtcggcacgg tggtgaagct gggagagtgc gtgccgaacc tgaaagtcgg
     4141 ccagcgcgtg ggtgccagcg ccatcgccgg gtgcggtgcc tgccccgcgt gcgcaaaagg
     4201 gcagtacacc tggtgcccga acaaccgctt ctacggcaac atgcacgccg aacgcttcct
     4261 cgccgccgcc aacgcctgcc atatcctgcc ggacgacgtg ccctgggatg ccggcttatt
     4321 gctcaccggc gacggcctcg gcgtgccgta ccacacctca cgcaaaatcg cctcgcggga
     4381 catcacaacg gtcgcggtat tcggcgtcgg gccgattgga ctcggcaacg tactgatgca
     4441 ggcccacctc ggccgccgcg tcatcgcggt ggatatttcc cccacccacc ttgagcacgc
     4501 gcggaactgc ggcgcagcgg aggtggtcaa cccgaaggac agcgacccgg tggaggcggt
     4561 gaaggcgctt accggcggaa aaggggccga tgtctgcatg gagtgcgccg gtcggcagga
     4621 gacggcaatg gcctgcttca gcgcggtgcg cactgccggc accgtggtgt tcaacggcga
     4681 gcaggggccg ctgccgctct cgcccagcag ccacttcatc cggcgcgata tcaccgccgt
     4741 cggttcgtgg ttttatcact tctccgagtt cggtgccatg ctcgagctct accgcaacgg
     4801 cctcgacg
//
LOCUS       DAOSOO010000229         4796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17788_length_4796_cov_5.852352, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000229 DAOSOO010000000
VERSION     DAOSOO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17788_length_4796_cov_5.852352"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(611..2398)
                     /locus_tag="PLT86_12715"
     CDS             complement(611..2398)
                     /locus_tag="PLT86_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT37516.1"
                     /translation="MPKLTINNRSIECEHGQTILQAAQAKGIHIPTLCYLEGKDPLGA
                     CRVCLVEVEGAKTLMPSCSTPVVEGMKIHTNNRKVREARKTVVELLLSEHEGECTLCP
                     RTEDCELRELAGQLGIDVVEYEGEKPPRTVDDSTPALVRDSGKCIKCRRCVTVCQEVQ
                     HVGALFPQYRGYETLIGPAFALQLDDVPCVQCGQCAAVCPVGAITEKSHIDAVWKALE
                     DKDKVVVVQTAPAIRAALGECFDLEPGTLVTGRMVTALRELGFDAVFDTNFTADLTII
                     EEGTELLTRLRRTVVDGEKGLLPMFTSCSPGWIQFAEYFYPEFLPNLSTCKSPQQMFG
                     AVAKTFYATEKGIPPENMVVVSVMPCTAKKFECQRDEMTASGYRDVDYVLTTRELGKM
                     ISAAGIDFVNLPDGEMDLPLGLSSGAADIFANTGGVMEAALRTVYELITGRPLPMENL
                     HVLPIEGLEGIKQASITIEKPVKEWAFLDNVTVKVAVAHGLSNAAKVLDGIKDGSLDF
                     HFVEVMTCPGGCIGGGGQPRFTTDETRKARIRAIYKEDEGKPVRKSHENPAIKEIYEK
                     FLKKPLGEKSHHLLHTKYQKRVKAGAKKK"
     gene            complement(2379..>4796)
                     /locus_tag="PLT86_12720"
     CDS             complement(2379..>4796)
                     /locus_tag="PLT86_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT37517.1"
                     /translation="GYAIGANMGYVYVRAEYPIAIDRLTKAIEDARRRGVLGENLFGS
                     GFGFDLDIRIGAGAFVCGEETALMASIEGKRGEPRPRPPFPAEKGLWGKPSLLNNVET
                     YANVPAIIFRGADWYASLGTAESKGTKVFALAGAVVNTGLVEVPIGISLGELIYDIGG
                     GIKNGKSFKAAQIGGPSGGCIPKEHLNVPMDYRPLTELGAIMGSGGLIVVDDDTCMVD
                     MARFFLEFVQEESCGKCVPCRVGTKRMLEIVTRICEGKGREGDIEKLIHLGNQIKETS
                     LCGLGQTAPNPVLSTIRYFREEYEQHIRDRHCEAGVCPSIVRAPCQSACPANVDVPGF
                     VSLVAEKKYAEALQLHRDRNPFASVCARVCFHTCEEKCQRAKLDSAVSIRAVKRFMVE
                     QEVMIQIPEVRENEENAKRKIAIIGAGPAGLSCAYFLARLGYKPTVFEAELRPGGMLV
                     QTIPAYRLPREVLAREIRMIERIGVDIQTGKRLGTDFTLASLKEEGYEAIFLGVGAPE
                     SLPIGIPGEDAEGVVQAADFLRTYNIRGSVPVGRKVIVVGGGNAAIDASRSALRLGAE
                     EITILYRRSQAEMPAYAEEIDEALHEGVKLMPLTHPLEILKDASGKVTGVRCTSMKLG
                     EFDRSGRRRPEEKAEFFNLEADQVIVAIGQALRPKMILKDMDIKLNSRNFIQADPITG
                     ETSVPGIFAGGDAVSGPYSVVGAIGAGERAAVGIDHYLTGAFHAFWRREKENTTHFDP
                     ESDPVPYGREPIRSLDVQRRRHNFDEVEMPWDEATAVRQARRCLRCDFGKHVPEKVGV
                     DDAQADD"
BASE COUNT          867 a         1566 c         1358 g         1005 t
ORIGIN      
        1 ccggggttgc cgtagatcgt catgacggac gtatccggca gcgcctgcga acgcgccgcg
       61 gcggcggtaa caaggattgc gaacagagaa gcgtacagag cacgcatggt cagcctcctc
      121 gcacccgaga gagggttcgg gtgcggcgaa aacagacgcg cgaactgggg agaacatcta
      181 tctacacgcg aatgtacggc gcaggccggg attgtcaaac gcggagtcgg aaaatcggaa
      241 aagggcccga cagagcgggc ccctccatcc tttgttccct ccccgcgctc gttggtagtg
      301 gagggacgcg ctccgccgcg tccgtttgcc gccacaaacg ccgcatccgg ggcaccgtag
      361 gggcaattca tgaattgccc ttacaccgcg aagcgaccct gcctgacgac catcatcctc
      421 cgtgtcaccg cctggtgccc tgccgacaaa aacgggtgga gccggcaatc caccggcccc
      481 accctgttcc attcctgccc gtcaaacgcg gtccacgcgg ggtccgctca ggcgcgtccc
      541 ttttgccgga aaaccgtagg ggcgacgcat gcgtcgcccc taccctcgga cctcgatcgt
      601 acgtgccccg ctacttcttc ttcgcacccg ccttcacgcg tttctggtac ttcgtgtgaa
      661 gcagatgatg cgatttctcg cccagaggct tcttgaggaa tttctcgtaa atctccttga
      721 tcgccgggtt ttcgtgcgat ttgcgcaccg gctttccttc gtcctctttg tagatcgctc
      781 tgatgcgcgc tttccgggtt tcgtccgtgg tgaagcgcgg ttgcccgcca ccaccgatgc
      841 atccgccggg gcacgtcatc acttccacga aatggaagtc cagcgagccg tcctttatgc
      901 cgtccagcac cttggccgcg ttcgagagac cgtgcgccac cgcgactttg accgtgacgt
      961 tgtcaaggaa cgcccactct ttgaccggtt tctcgatggt gatgctggcc tgtttgatcc
     1021 cctccagccc ctcgatgggg aggacatgga ggttctccat gggcagcggc cggccggtga
     1081 tgagttcgta gacggtgcgc agcgccgctt ccatcacgcc gccggtgttc gcgaaaatgt
     1141 ccgccgctcc tgaagaaagc cccagcggca ggtccatttc accgtcgggc aggttcacga
     1201 aatcaatacc cgccgcgctg atcattttgc ccagctcacg cgtggtcagg acgtagtcca
     1261 catcccggta cccgctcgcg gtcatttcgt cgcgctggca ctcgaatttc ttcgcggtgc
     1321 acggcatgac ggagaccacg accatgttct ctggcggaat acccttttcc gtggcataaa
     1381 aggtctttgc cacggcaccg aacatctgct gcggcgattt gcaggtggag aggttgggaa
     1441 ggaattcggg atagaagtac tccgcgaact ggatccaccc cggcgaacag ctcgtgaaca
     1501 tcgggaggag gcctttttct ccgtccacga cagttcttcg caggcgggtg agaagctccg
     1561 tgccctcttc gatgatcgtg aggtcggcgg tgaagttcgt gtcgaatacc gcatcgaacc
     1621 cgagttcgcg cagggcagtg accatgcgcc cggttaccag cgttcccggt tcgagatcaa
     1681 aacactcccc aagtgccgcc cggatcgccg gcgcggtttg aacaacgacg accttgtcct
     1741 tgtcctcaag cgccttccag accgcatcaa tgtggctttt ttcggtgatc gcgcccacag
     1801 ggcacaccgc cgcgcactgg ccgcactgga cgcacggtac gtcgtcaagc tggagtgcga
     1861 atgcgggccc gataagcgtc tcataccccc ggtactgcgg gaacaacgcg ccgacgtgtt
     1921 gaacttcctg acagaccgtg acgcatctcc ggcacttgat gcacttgccg gaatcgcgca
     1981 cgagcgccgg cgtggagtca tccaccgtac gcggcggctt ctcgccctca tactccacca
     2041 cgtcaatccc gagttggccc gcaagttcac gcagttcgca gtcctcggtt ctcgggcaga
     2101 gcgtgcattc cccttcgtgt tcgctaagga gaagctcgac gaccgtcttt cttgcctcgc
     2161 gcactttccg gttgttcgta tgaatcttca taccctcgac caccggagtg gagcacgacg
     2221 gcatgagcgt tttggcgcct tctacctcca cgagacagac ccggcatgcg ccgagggggt
     2281 cctttccttc gaggtagcag agcgtcggta tgtgtatccc cttggcctga gcagcctgaa
     2341 ggatcgtctg gccatgttcg cattcgatgc ttcgattgtt aatcgtcagc ttgggcatcg
     2401 tccaccccca ctttttccgg aacgtgtttg ccgaaatcgc accgcaaaca gcgccttgcc
     2461 tgacgcacgg cagtggcttc gtcccatggc atttctactt cgtcgaaatt gtgtcttctg
     2521 cgctgcacat ccagcgagcg aatcggttct cgcccgtacg gcacgggatc cgattccgga
     2581 tcgaaatgcg tggtgttctc cttttccctg cgccagaacg catggaacgc tccggtgagg
     2641 tagtgatcaa tgccaacggc cgcccgttcg ccggcgccaa tcgccccgac gacggaatac
     2701 ggcccgctca cggcatctcc gcccgcgaat atccccggca cggaagtttc gccggtaatc
     2761 ggatccgcct ggatgaagtt ccgggagttg agtttgatat ccatgtcctt cagaatcatc
     2821 ttgggacgca gcgcctgccc gatggcgacg atgacctgat ctgcttcaag gttgaagaac
     2881 tcggctttct cctccggccg ccgtcttccg ctccggtcga actcaccgag tttcatggag
     2941 gtgcaccgca ctcccgtaac cttcccgctg gcatccttga ggatttccag cggatgggtg
     3001 agcggcatca gcttcacgcc ttcgtggagc gcctcgtcga tttcttccgc gtatgccggc
     3061 atttcggcct ggctacggcg gtagagaatc gtgatttcct cggcgccgag acgcagagca
     3121 ctgcgggagg catcaatcgc cgcattgccg ccacccacca cgatcacctt tctgccgacc
     3181 ggcaccgatc cgcggatgtt gtatgtgcgg aggaaatccg ccgcctgaac cacgccctcc
     3241 gcgtcctctc ccggaatgcc gatgggcagg gattccggtg cgcctacgcc gaggaagatc
     3301 gcctcgtacc cctcttcctt gaggctcgcg agggtgaaat ccgtaccgag acgctttccg
     3361 gtctgaatgt cgaccccgat ccgctcgatc atgcggattt cgcgggccag aacttccctc
     3421 ggcagccggt acgcagggat tgtctggacc agcattccac ccggacgaag ctccgcttca
     3481 aacacggtcg gtttgtaccc gagccgcgca aggaaatacg cacaggagag ccctgccggg
     3541 cccgcaccga tgatggcgat tttccgcttc gcgttctcct cgttttcccg cacttcggga
     3601 atctggatca tcacttcctg ttcgaccatg aaacgcttga ccgcgcgaat agacactgcg
     3661 gaatcgagtt tcgcgcgctg gcacttttcc tcgcacgtgt ggaagcacac tcgcgcgcac
     3721 accgaggcga aaggattgcg gtcgcgatgg agttgcagcg cttccgcgta cttcttctcc
     3781 gcaacaagcg atacgaaccc cggcacatcc acattggccg ggcacgcact ctggcacgga
     3841 gcccggacaa tggacggaca gacgcctgct tcgcagtgcc ggtcgcggat gtgctgctca
     3901 tattcctccc ggaaataccg gatcgtgctg agaaccgggt tgggagcagt ttgacccagg
     3961 ccgcacagtg acgtctcctt gatctggtta ccgaggtgaa tcagcttctc gatatcgcct
     4021 tctcggccct taccctcgca gatgcgggtg acgatttcca gcatccgttt ggtgccgacg
     4081 cggcatggca cgcatttgcc acaagattcc tcctgcacaa actcgaggaa gaaccgcgcc
     4141 atgtccacca tgcaggtgtc gtcatccacc acgataaggc cgccagagcc catgatggcg
     4201 ccaagctcgg taagcggccg gtagtccatc ggaacgttga ggtgctcttt cggaatgcac
     4261 cctcctgatg ggccgccgat ctgggcagct ttgaacgatt tcccgttctt gatacccccg
     4321 ccgatgtcgt aaatgagctc gccgagactg attccgatcg gaacttccac cagaccggtg
     4381 ttcaccaccg cgccggccag cgcaaacacc ttcgtgcctt tactttccgc cgttcccagc
     4441 gaggcatacc agtccgcacc ccggaaaatg atcgccggga cgttggcgta cgtctccacg
     4501 ttgttgagca ggctcggttt gccccagaga cccttctctg ccgggaaggg cgggcgggga
     4561 cgcggttcgc cgcgttttcc ctcaatggag gccatcagcg ccgtttcctc gccgcatacg
     4621 aacgcgcccg cgccgatgcg gatgtcaagg tcgaagccga atccgcttcc aaacaggttc
     4681 tctccaagca ctccgcgccg tctggcgtct tctatcgctt ttgtcagccg atcgatcgca
     4741 atggggtact cggcacgcac gtaaacatag cccatgtttg cgccgatggc gtaccc
//
LOCUS       DAOSOO010000230         4750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_17994_length_4750_cov_4.089031, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000230 DAOSOO010000000
VERSION     DAOSOO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17994_length_4750_cov_4.089031"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /gene="ruvA"
                     /locus_tag="PLT86_12725"
     CDS             complement(<1..502)
                     /gene="ruvA"
                     /locus_tag="PLT86_12725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT37518.1"
                     /translation="MIAYLAGTLAEKSPGFVVVDVHGVGYRVAIPLSTYEKLGEIGSS
                     IKLLTYLSVRENAFDLFGFSSALERDLFLRLIEISGVGPRLAINVLSGMEPMLFRRAV
                     LTGDVKALSRIPGVGKKTAERLLLELSGAFQGGGELAAMGVTPGPGKDAADAQTEAVL
                     ALVNLGF"
     gene            complement(546..1091)
                     /gene="ruvC"
                     /locus_tag="PLT86_12730"
     CDS             complement(546..1091)
                     /gene="ruvC"
                     /locus_tag="PLT86_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT37519.1"
                     /translation="MTVQRVLGVDPGTIVCGYGIIDVAGSRYTYVTSGVIRPLRNAPM
                     PDRLCTVFRGIASLIEEYRPTVLAVEEAFYGDNPKTAIKLGQARGVILVQGVLAGMHV
                     SEFSPRFIKQAVVGVGSATKEQIQYMVARILKLPSPPQPTDAADALATALCFAVQPHV
                     EKADDRPLTRAQRQMKALGIL"
     gene            complement(1179..1928)
                     /locus_tag="PLT86_12735"
     CDS             complement(1179..1928)
                     /locus_tag="PLT86_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT37520.1"
                     /translation="MSGHSKWATIKRKKGKNDAQRGRIFTKLIKEIAIAARNGGGNPD
                     ANPRLRRAIDTAKANSMPMENVERAIKRGTGELEGVSYEEITYEGYAPGGVALLIESV
                     TDNRNRTVSELRNLLSKNGGNLAEAGAVAWVFEAKGSISVEKSAGTEEDVFNIALEAG
                     AEDMETEGETYEITTPLSELDAVRRALEDAGIAVASAEPARVPTTTVKVEGKQAEQVM
                     RLLEALDDHDDVQKVWDNSDIDEKVLAELAA"
     gene            complement(2506..>4750)
                     /locus_tag="PLT86_12740"
     CDS             complement(2506..>4750)
                     /locus_tag="PLT86_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT37521.1"
                     /translation="PALFLLSNAAAQSDTTVTGSPISAPSPHTGDILSVALTQDGQTI
                     VSGSKDHTVKLWRLSDGANLRTLIGHTSSVTCVAVTPDGQMIVSGSGDGTVKVWRTSD
                     GQLLRSFVPSGQAGPGGGTATVTSVAVSPDGQTLISGGADNTVRMWKLADNELLWTSR
                     HVDAVSAVAITPDGQTVVSGSWDRTVRVWRASDGQLLRMLSGHRGMVRSVAVTLDGQT
                     IVSGSDDNTVRVWSLPTGDLLRTLPAHTYVVQAVAVTPDGQTVVSAGRGGTGGTIEVW
                     SLATGELVRTLTGHTALVNCLAATPDGKNIVSCSDDLTIRVWRLSSGEIIPLFKGHTG
                     IVRSVAITPDGQTVVSGGDTTIKLWRYSDGRLLRTLTGATRVVTSVAITPDGETIVSG
                     SGLSECTIKIWRLATGELLRTLPVPGSGDAGHTGQVWTVAITPDGQTIVSSGGTNDRT
                     VKVWRLSDGKLLRTLTGHTSRVTCAAVTGDGQTIVSSSYDNTIKVWRLADGELLRTLP
                     LPGSSDAGHLGGIYTVAIAPDGQTIYSGGYDGTIKVWRLATGEFVRSLQGHTACVTTI
                     AHTPDGRSVVSGSEDGTIRVWEMVQGTLMQTLLCHSAGVMSLAVAPDGRNVISGGADA
                     GVSSGTVQAALWRWAIAPPLGVDPSAGDNNPRQFTLSQNAPNPFNPSTTLRFGLPEAG
                     HVTLAVYDVRGALVCTLVDQTLVSGVHAVQWDGYDAMGREVASGVYLARLTAPQGVVT
                     RRMVLLR"
BASE COUNT          926 a         1531 c         1370 g          923 t
ORIGIN      
        1 ggaagcccag attcaccagc gcaagaacag cttccgtctg cgcatccgca gcgtccttcc
       61 ccggccccgg cgtgacgccc atggcagcaa gttcgccgcc gccctggaac gctcccgaca
      121 gttccaggag caagcgttcc gcggtctttt tgcccacccc cggtatccgg gagagcgctt
      181 tcacgtcgcc ggtcagaaca gcccgtcgga ataacatcgg ttccatcccc gagagcacgt
      241 tgatcgcaag ccgcggaccg accccgctga tttcgatcaa tctcaggaag aggtcgcgtt
      301 ccaaagcaga actgaaaccg aacaggtcga acgcgttctc gcggacgctg agatacgtca
      361 ggagcttgat cgagctcccg atttcgccaa gcttttcgta cgtggaaaga ggtattgcaa
      421 ctcggtaccc caccccgtga acatcgacga ctacgaagcc gggggatttc tccgcaaggg
      481 ttcccgcgag atacgcaatc atacaaactc cgcgcacgcc agcgaggaaa tggcaagtat
      541 ctgcttcaca gaattccgag ggctttcatc tgcctctggg cgcgtgtgag cggccggtca
      601 tcggcctttt cgacatgcgg ttgcacggca aaacacagcg ctgttgccag agcgtcagct
      661 gcatcggtcg gttgcggggg cgaaggcagt ttcagaatgc gcgcaaccat gtattgaatc
      721 tgctccttgg tggcacttcc gacgccgact actgcctgct tgatgaaccg gggactgaac
      781 tctgaaacgt gcatccccgc aagcacgccc tgtaccaaaa taaccccgcg tgcctgcccg
      841 agcttgatgg cggtcttcgg attgtccccg taaaacgcct cttccaccgc aagcacggtg
      901 gggcggtact cttcaatgag cgatgcaatt ccccggaaca cagtgcacag gcggtctggc
      961 atgggtgcgt ttcggagcgg gcgaatgacc ccggaggtca catacgtata ccggctgcct
     1021 gcaacatcga taataccgta cccgcagacg attgttccag ggtcaacgcc gagaacccgt
     1081 tgtaccgtca tcaggaaacc tgcagaaacg aagaaccccg cgcatcgacc tcgcggcaga
     1141 gaaagccgtg ccggtagtac gcgcggggaa ccgttgaatc aggccgcaag ttcagccaga
     1201 accttctcgt ctatgtcaga attatcccag accttctgga catcgtcatg gtcgtcgagt
     1261 gcttcaagca accgcatgac ctgttctgcc tgcttcccct caactttcac cgtggtggtg
     1321 ggaacgcgcg ccggttccgc actcgcaacc gcgatgcccg cgtcttcaag ggcgcgacga
     1381 actgcatcca gttcgctcaa gggtgttgtg atctcgtatg tttcgccctc ggtttccatg
     1441 tcctccgccc cggcttccag cgcgatgttg aacacatcct cttctgtgcc ggcggatttt
     1501 tccaccgaga tgctcccctt cgcctcaaac acccatgcga ccgcacccgc ttccgcgaga
     1561 ttaccgccat tcttgctcaa caggtttcgg agctccgaaa cggtgcgatt gcgattatct
     1621 gtcaccgatt cgatgagaag agcgacaccg ccgggggcat atccttcgta ggtgatctcc
     1681 tcgtacgaaa ctccctcgag ctcgcccgtt cctcgcttga tcgcccgctc gacgttctcc
     1741 atcggcatgc tgttcgcctt ggcggtatcg atcgcgcggc gcaaccgagg gttcgcgtcg
     1801 gggttgcccc cgccgttccg ggcggcaatg gcgatttcct tgatcaactt cgtaaaaatg
     1861 cgcccgcgct gggcgtcatt cttccccttc ttgcgcttaa tggtcgccca ctttgaatgt
     1921 cctgacatgg tattactcct gaatccggtt cgacaacgac ggcgaacgat caaccacgaa
     1981 cgacaccagg ccgttcagag ggagggcgcc tgtgcgtatt tcgaaccaga agaagaccaa
     2041 aacaacgagg cgcgacagga aagcctcgtc cgacaggaca actgcacatg aaatccatct
     2101 ccagcgcagg ggaattaacg cgcgcgtagc aggtaccaaa agtacggttt cacaccggta
     2161 ttcgccaaga tgcgccggat tgcgcgattc gcccggctga agccgtagta ctgcggggga
     2221 acggcaggga caaggccgga acgcggcagt agaagtggaa aaccagacac gcagtcgttg
     2281 gcgagtggtg tggattgcgc atgttggcgc gtcgccagcc aagcttgccg gcgcaggcat
     2341 tttgacgcac acacgggacc gccactacac caggaaccgc cctacctcca gacacacctt
     2401 agcgcggtcg ttgagcctgt cgaaacgccg cgcggggctt cgacaggctt cctgcgcgac
     2461 gcacacgctc agccaccacg cgctcctacc tcaccaggct gcgcgtcagc gtagcagcac
     2521 catccgcctc gtcaccacgc cctgtggggc ggtcaggcgc gccagataca ccccgctggc
     2581 gacctcacgc cccattgcgt catacccgtc ccattggacc gcatggaccc cggaaaccag
     2641 tgtctggtcc accagtgtgc aaaccagcgc accccgcacg tcgtacactg ccagcgtcac
     2701 atgccccgcc tcgggcaggc cgaaccgcag cgtggtggac gggttgaacg ggttcggcgc
     2761 gttctgggag agggtgaact gccgcggatt gttgtctcct gcgcttgggt caacgccaag
     2821 cgggggggca atcgcccaac gccagagggc cgcctgaacc gttccggagg atacgccggc
     2881 atcggccccg ccggagatga cgttcctccc gtcgggagcg acggccaagc tcatcacgcc
     2941 ggcactgtgg caaagcagcg tttgcattag tgtgccttga accatctccc aaactcggat
     3001 cgttccgtcc tcgctcccgg agacgactga tcggccgtca ggcgtgtgag cgatggtcgt
     3061 cacacaggcg gtgtggcctt gaagtgagcg cacgaactcg cccgtcgcaa gccgccacac
     3121 tttgatcgtg ccgtcgtaac ccccagagta gatcgtctga ccgtcaggcg caattgccac
     3181 agtgtagatt ccccccaaat ggcctgcatc actgctgccg ggcagaggta acgtgcgcag
     3241 taactcgccg tcggccaacc gccaaacttt gattgtgttg tcataactcg acgacacgat
     3301 cgtttgaccg tcacctgtta cggcggcaca tgtgaccctc gacgtgtgac cagtgagcgt
     3361 ccgcaagagt ttgccgtccg agagccgcca gactttcacg gttctatcgt tcgtcccccc
     3421 acttgagaca atcgtttggc catcgggtgt gatagcgacc gtccagacct ggcctgtgtg
     3481 cccggcgtcc ccgctaccgg gtacgggaag cgtgcggaga agctcgcctg tcgcaagccg
     3541 ccatatctta atggtgcact cgcttaggcc ggaacctgag acgatggttt caccgtcagg
     3601 cgtgatagca acgcttgtga ctacgcgggt tgcccccgtg agtgtgcgga gaagccggcc
     3661 gtcagaatag cgccacagtt tgatcgttgt gtcgccaccc gacacaaccg tctggccgtc
     3721 aggcgtaata gcgacgctgc gcacgatacc tgtgtgccct ttgaacaggg gaatgatttc
     3781 gccactcgat agccgccaca ctctgatggt caggtcatcg ctgcacgaca cgatgttttt
     3841 gccatcgggc gtcgcggcga ggcagttcac caaagcagtg tggccggtga gcgttcggac
     3901 gagttctccc gtcgcgaggc tccatacttc gattgtgccg cctgtgccgc ctcgcccggc
     3961 tgagacgacg gtttggccgt caggcgtcac cgcaacggcc tgcacgacgt atgtgtgcgc
     4021 cggcagggta cgcaggaggt ccccggtcgg gaggctccac actcggactg tgttgtcatc
     4081 actccccgac acgatcgtct gcccgtccag tgtgacggca acactgcgca ccatgcccct
     4141 atggccggaa agcatgcgca acagctgacc atcggaggcg cgccaaaccc tgaccgttcg
     4201 gtcccagctc cctgagacta ctgtctggcc atctggcgtg atggcaactg ctgataccgc
     4261 gtcaacgtgc ctgcttgtcc acaaaagctc gttgtccgcg agcttccaca ttctgactgt
     4321 gttgtcagcg ccgccggaga taagggtctg accatcgggg ctcaccgcca cgctggttac
     4381 cgttgcagtt cctcccccag gcccggcctg gccggatggg acgaacgacc gcaaaagttg
     4441 gccgtcacta gtccgccaaa ccttgactgt tccgtcccca ctcccggaaa cgatcatctg
     4501 gccatcggga gtcactgcga cgcaggtgac gctgctcgtg tgcccaatga gagtccgcaa
     4561 attggcgccg tcggacagcc gccagagttt caccgtgtgg tctttgctcc cagagacgat
     4621 tgtctggccg tcctgcgtca aagcaacgct gagtatgtcg ccagtatgtg ggcttggcgc
     4681 cgagatcggc gaacctgtga ctgtcgtgtc ggattgggca gcggcgttgc tcaggaggaa
     4741 gagcgcgggc
//
LOCUS       DAOSOO010000231         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18185_length_4716_cov_4.934992, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000231 DAOSOO010000000
VERSION     DAOSOO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18185_length_4716_cov_4.934992"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..93
                     /locus_tag="PLT86_12745"
     tRNA            16..93
                     /locus_tag="PLT86_12745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Pro,seq:tgg)
     gene            complement(154..1833)
                     /locus_tag="PLT86_12750"
     CDS             complement(154..1833)
                     /locus_tag="PLT86_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOT37522.1"
                     /translation="MQSNAMHARKLALVGLFLAYFASGCSLILRLPPSTTHMVRMRDG
                     TELATDVYFPKGRLHRMPTILIRNTYNKGAVVNGIKTSSVIAKTFTDKGYAVVVQDTR
                     GRFASAGVDSIFRTDGAGPNQDGYDTIEWITRQNWSNGAVGMWGMSALGITSYMAVAS
                     GHPALKAAYVVMCGSNLYDDVFFPGGVFRKDLVERWVTGQGRADYLPVLREHASYDDY
                     WEAMNLSVHAPRAHTAVYHCGGWFDVMSAGQTNGFQALHERGGEGARGRQLMVMGPWS
                     HHGMGRKQGDVEFPGNNKDVNPMGDAVAWFDSRLKGVPTRVDSVAAVRYYLMGASGDS
                     SGAGNRWLNAPTWPPPESQDVAFYLAEDRALTRDAPTSEAGALSYEFDPRNPVPTLGG
                     LNLYPPIGPRDVRSVESRPDVLSFTTPPLEAPVAIAGNVHVKLWASSSARDTDFTAML
                     CDVYPDGRSVSILDGAIRACHRNTTREEHLLAPGEVYEFTINLWDTALAFERGHSIRV
                     DISSSNFPRFEANPNTGTPSGSDTTRVVAKNTIYFGAQRPSALILPVIPLE"
     gene            complement(1839..2033)
                     /locus_tag="PLT86_12755"
     CDS             complement(1839..2033)
                     /locus_tag="PLT86_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37523.1"
                     /translation="MKAKVICVQTTDPGRIGDLLQGKLNEFLAAVPSANVHSTHMAAL
                     DIGGPSQADVLVVYTVFYTE"
     gene            complement(2060..2551)
                     /locus_tag="PLT86_12760"
     CDS             complement(2060..2551)
                     /locus_tag="PLT86_12760"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOT37524.1"
                     /translation="MDVDPTWRDDGGVRLNVVLVEPEIPQNTGTIARLCAATACALHL
                     VEPLGFDVSDRTLKRAGLDYWQHVTVSVHRSFGALQEAYPGARFWYTSKKAHVPYTQA
                     KFHPDDFIVFGKETTGLPADLLDANSEWSLAIPMFGPVRSLNLANAVSIVLYEALRQL
                     RGF"
     gene            complement(2600..3922)
                     /gene="rlmD"
                     /locus_tag="PLT86_12765"
     CDS             complement(2600..3922)
                     /gene="rlmD"
                     /locus_tag="PLT86_12765"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT37525.1"
                     /translation="MNAHEPFPVSVGDVIELDVHDQPYGDAALGRFSRLAVFVPGAVA
                     GDRVLARVTEVHTRYARADLISVVTSSPDRVPAECPLAGRCGGCQWQTTAMARQHAAK
                     HGAVVEALRRVGKWEEPPVRDILPSGEPWRYRNKARYAVVTGAAGTGIGYFGTRTHDL
                     VSISRCPLNMERLDALLDAAHDLLINNPRFDDLRATTNSLTGRTSAATGETVLRVAAK
                     AASDWGAFAEALRQRIPFVSGVVVAGHPRRGDRVVWGSGSLTECVRGNEYRVSAASFF
                     QVNPFAAGDLVDAVAEAAGLQGSEILLDAYGGVGLFSFALCRDAARVFLIESDASALR
                     DARAIADAFRAQNVTIVDGSVERAARRIPKADTIICDPPREGAGPGGIRALVSLKPRK
                     LVYVACDPASLARDSNILRAEGFRLAWAQPLDMFPHTYHVETVALFER"
     gene            complement(3919..4368)
                     /locus_tag="PLT86_12770"
     CDS             complement(3919..4368)
                     /locus_tag="PLT86_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37526.1"
                     /translation="MGMWTGFHFGSTPRVSWYVRIALYATILLGTAFGLNRLRGRIPQ
                     AFGLTPIRVEVLSLERFRDRDLRGGKENTFGVVARITGRKSPSASVSLRQFVLKTESG
                     QLVRPYASSLQFDHLGNLAIAHADTFTGVFLFSVPSTEKPSEFWWNP"
     gene            complement(4390..>4716)
                     /locus_tag="PLT86_12775"
     CDS             complement(4390..>4716)
                     /locus_tag="PLT86_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT37527.1"
                     /translation="MMVIPYRHVSGLSDLTSEERLEIMEGAATAVDVLRETFHCDGNN
                     LGFNLGRPAGAGIDAHLHMHVVPRWNGDTNYMAVLDDTRVISEALEETYRRLRAAYRE
                     RGLAPA"
BASE COUNT          956 a         1527 c         1329 g          904 t
ORIGIN      
        1 ttcatacacc cttgtcgggg cgtggcgcag tccggctagc gcatctgctt tgggagcaga
       61 gggtcctggg ttcaaatccc ggcgccccga ccaccctctc ctgcgttcga atccttcgtc
      121 ccgaatcacc tcctctgcac cgtcctgcac agttcactca agtggaatca ccggcaggat
      181 aagcgcagaa ggcctttgcg cgccgaagta gatagtgttt tttgccacca cgcgggtcgt
      241 gtcggaaccc gacggagtgc cggtatttgg gtttgcctcg aaccggggga aattgctgct
      301 cgatatgtct acccggatcg aatgaccacg ttcaaacgca agcgcggtgt cccagaggtt
      361 tatcgtgaat tcatagacct ctcccggggc gagcaggtgc tcttccctgg tggtgttccg
      421 atggcaagcc cgaatcgcac cgtccagtat cgaaactgac cggccgtcgg ggtacacgtc
      481 acagagcatt gcagtgaaat cggtgtccct cgcggaagaa gacgcccaca actttacgtg
      541 gacgttgccc gcgatggcga ctggtgcttc cagaggaggc gtggtgaaac tcagcacgtc
      601 gggacgcgat tccacagacc gtacgtcgcg cgggcctatc ggggggtaca gattcagtcc
      661 acccagcgtg ggaaccgggt ttcgcggatc gaattcgtag ctcaaagccc ctgcttcact
      721 ggtcggcgcg tcgcgggtca aagccctgtc ctctgcgagg taaaacgcga cgtcctgcga
      781 ttccggcggt ggccatgtcg gtgcattcag ccagcggttg ccagccccgg agctgtcccc
      841 cgaagcaccc atcaggtagt accgaaccgc cgccacgctg tccacccggg tgggaacgcc
      901 tttcagccgg ctgtcaaacc atgccaccgc atcccccatc gggttcacgt ccttgttgtt
      961 tccggggaac tcaacatctc cctgcttcct gcccattccg tggtgcgacc agggccccat
     1021 caccatcagt tgccttccgc gcgctccctc gccaccgcgt tcgtgaagtg cctggaaccc
     1081 gtttgtctgc cccgcgctca tcacatcaaa ccagccgccg caatggtaca cggccgtgtg
     1141 tgcgcgggga gcgtggaccg aaagattcat ggcttcccag taatcgtcat acgacgcgtg
     1201 ctcccgcagc acaggcaggt agtccgctcg gccttggccc gtaacccatc gctccaccag
     1261 atctttgcgg aacaccccgc cggggaagaa cacgtcatcg tagaggttcg agccgcacat
     1321 caccacgtac gccgccttaa gtgcagggtg cccgctggcg accgccatat acgatgtgat
     1381 cccaagggcc gacatgcccc acatcccgac tgcgccgttg ctccagttct ggcgcgtgat
     1441 ccattcaatc gtatcgtacc catcctggtt cggccccgcg ccgtctgtcc ggaaaatcga
     1501 atcaacgccg gcggaggcaa acctgccccg tgtatcctgc accacaaccg cgtatccctt
     1561 gtctgtgaac gtctttgcga tcacggatga ggtcttgatg ccattcacca ccgcgccctt
     1621 gttgtacgtg ttcctgataa gaatcgtcgg catccggtgc aggcgcccct tcgggaagta
     1681 aacgtctgtc gccagttctg tcccgtctcg cattcgcacc atatgcgtgg tggagggggg
     1741 gagcctcaga atcaggctgc accccgacgc aaaatacgcc agaaacaggc cgacaagggc
     1801 caattttcga gcgtgcatgg cgttactctg catccggttc actccgtgta gaacactgtg
     1861 tacaccacca gcacgtctgc ctgcgaaggg ccgccgatgt ccagtgctgc catgtgggtg
     1921 gaatgcacgt tggcggaggg taccgccgcc agaaactcgt tcagtttgcc ttgcagtaga
     1981 tccccgatcc gtccggggtc agtggtttgt acacagatca ctttggcctt catcgaagtc
     2041 ctcctgaaca acaggcgctt cagaaacccc gcaactgccg caacgcttca tacagaacaa
     2101 tgctcactgc gttcgccaga ttcaggctcc gcaccggacc gaacatcgga atcgccagcg
     2161 accactcgga attcgcgtca aggaggtccg cgggcagacc cgtcgtttct tttccaaaca
     2221 cgataaaatc atcgggatgg aacttcgctt gggtgtacgg aacgtgcgct tttttcgagg
     2281 tgtaccagaa tcgcgcacca gggtatgcct cctgcaacgc accgaaggat ctgtgtacag
     2341 ataccgtcac gtgctgccag taatccagcc ccgcgcgctt cagcgtgcga tcgctcacgt
     2401 cgaatccgag cggctccaca agatgtagag cgcacgccgt ggcggcgcac agccgcgcga
     2461 tggtgcccgt attctgagga atctctggct caacgagcac aacgttgagt ctaactccac
     2521 cgtcatcgcg ccacgtggga tcaacatcca cgggctccac gaatggatgg ggacgtcgac
     2581 gatcgctagg caccggaaat cacctctcaa aaagcgctac tgtttcaacg tgatacgtgt
     2641 gaggaaacat gtccagcggc tgcgcccatg caaggcggaa tccttccgcg cgaaggatat
     2701 tggagtcgcg tgccagactc gcaggatcac atgcgacata caccagtttc cgcggcttga
     2761 gagagacaag cgcgcggatt ccacccggtc ccgccccctc gcgcggcgga tcacagatga
     2821 tcgtgtcggc cttgggaatc cggcgtgccg cgcgttccac ggaaccatct acaatagtaa
     2881 cattttgggc acggaaggca tccgcgattg ccctggcatc acggagtgcc gatgcgtctg
     2941 attcgatgag gaaaacccgc gctgcgtcgc ggcacaacgc aaaagaaaaa agccccacgc
     3001 ctccgtacgc gtccagaagt atctcgctcc cctgcaaacc cgcagcttct gcaactgcat
     3061 ccacgagatc cccggcagcg aacggattca cctgaaaaaa agatgccgcc gacactcgat
     3121 actcgttccc tcgtacacat tcggtcaggc tgcccgaacc ccacaccaca cgatcaccgc
     3181 gcctcggatg acccgcaacg acgacgccgc tcacgaacgg gatacgttgc cgaagagctt
     3241 cggcgaaagc cccccagtcg gacgccgcct tggcggccac ccgcaacact gtctcacctg
     3301 tcgctgcgct cgttctcccg gtaagggagt tcgtggtcgc gcgaaggtcg tcaaatcggg
     3361 gattgttgat caaaaggtcg tgcgcagcat cgaggagtgc gtcaagccgt tccatgttga
     3421 gggggcaacg cgaaatactg acaagatcgt gggttcgcgt gccgaaatac ccgattcctg
     3481 tccctgcggc tcccgtaacg acagcgtacc gggccttgtt ccgatacctc cagggctcgc
     3541 cgctcggaag gatgtcacga accggcggct cttcccattt cccgactcga cggagcgctt
     3601 ctacgaccgc cccgtgcttc gccgcatgct gccttgccat tgcggtcgtt tgccactggc
     3661 agcctccgca acggcccgca agtgggcatt cggccggaac acggtccgga gatgaggtaa
     3721 caacggagat caggtccgcc cgcgcgtacc gggtatggac ttcagtgaca cgcgcaagaa
     3781 cgcggtctcc cgcaaccgcg cccggcacga ataccgccaa tcgagaaaaa cgcccgaggg
     3841 ccgcgtcgcc atacggttgg tcgtggacgt ccaactcaat gacatccccg actgacacgg
     3901 ggaacggttc gtgtgcgttc acgggttcca ccagaactca gacggtttct cagtggaggg
     3961 aacggaaaac aggaagacgc cggtgaacgt gtccgcatgc gcaatcgcca ggttacccag
     4021 atggtcaaac tgcagggaac tggcatacgg gcggaccagc tgcccgctct cggtcttgag
     4081 cacgaattgt cggagggata cgctggcgga agggctcttt cttccggtga ttctcgcgac
     4141 gactccgaac gtgttctcct tgcctccgcg gaggtctcgg tcccgaaatc gttccagcga
     4201 gagcacttcc acccgaatag gcgtgaggcc aaacgcttga ggaatccggc cacgcagacg
     4261 gttcaacccg aatgcggttc ccagaagaat cgtcgcatag agggcgatcc gcacatacca
     4321 ggacacgcga ggtgtgcttc cgaagtgaaa acccgtccac atccccatcg tcggttcact
     4381 gtcccggcgt catgcaggcg caagtccacg ctcgcggtat gcagctctga gccgtctgta
     4441 ggtctcctcc agagcctccg aaatcaccct cgtgtcgtca agaaccgcca tgtagttcgt
     4501 gtcaccgttc cagcgaggaa ccacgtgcat gtgaaggtgt gcgtcgatgc ctgcccccgc
     4561 aggcctgcca aggttgaacc cgaggttgtt gccgtcgcag tgaaaggtct cccgcaacac
     4621 gtccaccgcg gttgccgccc cttccatgat ttcgagccgc tcctcgctcg tgagatcgga
     4681 aaggccggag acgtgcctgt acggaatcac catcat
//
LOCUS       DAOSOO010000232         4699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18250_length_4699_cov_5.354436, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000232 DAOSOO010000000
VERSION     DAOSOO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18250_length_4699_cov_5.354436"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..624)
                     /locus_tag="PLT86_12780"
     CDS             complement(181..624)
                     /locus_tag="PLT86_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37528.1"
                     /translation="MMSCHEVQSLLDDFGRGAIRENEAGQLSIHLRECAECRAEFELE
                     SRLVRIGANAPVFAPPADFAAKVVAEWQRVAAVETVTPWEILKNALSEVFRAASDPIL
                     QPLLLVRCEIDETLANARQTLCKASEIVSMTLKQVFFGRFVPENK"
     gene            complement(621..1172)
                     /locus_tag="PLT86_12785"
     CDS             complement(621..1172)
                     /locus_tag="PLT86_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT37529.1"
                     /translation="MHSDQELVEACCRGDRAAAEALVRRYQSAVCTLAVRMLGDTQEA
                     EDAAQTVFLNALRSLHLFRGGSSLKTWLYRITVNECTARAKLRKRFPRPASDDDPVAS
                     LPDQNRLASDLANDEEQETRNAVAALPESYRLPIMLRYFEDLSYEETAQALAVSVETV
                     KIRLFRAKAMLKTLLAEREDLRS"
     gene            complement(1334..2032)
                     /locus_tag="PLT86_12790"
     CDS             complement(1334..2032)
                     /locus_tag="PLT86_12790"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT37530.1"
                     /translation="MNTVPAGFPAPKIGNQTAVLTHRTIRDHVYHLYRVPDLDRLVDE
                     IDPDTWKQDEKMPYWAELWPSGVALAELLASDAAALRHARVLELGCGLGLPSIVAAHL
                     GAQVTATDYYPEALDLLHVNAALNGVSLRTREVDWRTPPDLGRFDVVIAADVLYERWQ
                     TPAMVDMLARTVLPNGTAIIVDPERVTARDFAGAATFRGFRVETSYRRAENEAIPLII
                     YRLTWRTFPEHSKQ"
     gene            complement(2029..2571)
                     /locus_tag="PLT86_12795"
     CDS             complement(2029..2571)
                     /locus_tag="PLT86_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37531.1"
                     /translation="MRFFPRTRKRVDEATPEWSVLWAVNVARVESVFYTVEAITANNV
                     CPVRLFAPGSRTELVRLPALSSGACLHPFSRIRLVEASATGFCFQAFCSSQSAALAIR
                     GTRGSGVVTLALESRSGERPSAVVDGDIAPRVTLMSRHGICEIRNRILVMEAASEPLG
                     EEPRGSVWACADLVLGGSSA"
     gene            complement(2568..3308)
                     /locus_tag="PLT86_12800"
     CDS             complement(2568..3308)
                     /locus_tag="PLT86_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37532.1"
                     /translation="MIGATIVLTAVYWLSDLAVAMWMGGLLTVSLLLGLPLDDSPAGD
                     PMLLANGASTAVEMILRPRPYIFGAALILLASVVIGVILERRARRSFAWWVIWLLRSL
                     SGLFMFIVAIYSFGNLDPYITKMQNSVDTLKKSLAVQASAESAVQDGSQELSRVMENP
                     YDAATVETLKADFTALQNRYRHWMWIQLLLGSAVLFAGALKQAAWRRSWGKEKEQAAE
                     GPEKESPATPQSPEKDGRNPEGETPQPR"
     gene            complement(3338..>4699)
                     /gene="bshC"
                     /locus_tag="PLT86_12805"
     CDS             complement(3338..>4699)
                     /gene="bshC"
                     /locus_tag="PLT86_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOT37533.1"
                     /translation="AKPAEMMTRAWARALESLLGEAVVPVFWIAGDDHDYEEVARVAF
                     PARTGFPHHVELPRSLVSGAPSARVAELRFVGEITGWADQTVSLLPGGQGRDQAADAI
                     REAFAPGATLVDAFARLMVTWLGRYGIIFANPADRRFKGLLQPLFSRCSSDPVGFRDA
                     FVEQSSRVRTEGYVPQIVTSPEATLFFWDDETGNRRRVDFTPTQGFFWREGATVHTIP
                     EFEKIASVSPERISPNAAFRPVSGDSVFPTLVHVMGPGEIAYMAQVRALYDMSGVPMP
                     LIAARAGFTVVPQEIGSWLQSRSLAIEEALQPLDVWRHTLAEREKRSSLGGRIAETRR
                     ALDETYEKLEHEVRNVLPGMDTAVLSARIKTQGLMNKLEAKLDQGFRRRVRETMPELE
                     RISSFVFPGGVPQERVYSAAPFVARYGEAFFADLLSQIRPFDPVHTCMWGESFGLDDL
                     PVS"
BASE COUNT          959 a         1484 c         1371 g          885 t
ORIGIN      
        1 cagattcgga accgccttgt gtgctattcg aagagcctgt ttgccgggac ccaggagggc
       61 tgctgtcgcg accacgagca caaacaggat ccccacgacg agaactgtcg tgatgcccag
      121 aaccacgact ttgccagtgc tcattgcggc caatgcttcg aacatggtgt gcccctttct
      181 ctacttgttt tccggaacaa accggccaaa gaatacctgt ttcagcgtca ttgagacgat
      241 ctcggatgcc ttacacagcg tctgcctggc attcgccagc gtttcatcta tttcgcacct
      301 cacgagcaga agaggttgga ggatggggtc ggacgccgcc ctgaacacct ccgatagcgc
      361 attcttgagg atctcccacg gtgtcactgt ttccaccgcg gcgacccgtt gccactcagc
      421 aacaaccttc gcggcgaaat cggccggggg cgcaaacacc ggggcattgg ctccgatcct
      481 gacgaggcgc gattcaagct cgaattccgc ccggcattcg gcacattccc ggaggtggat
      541 tgagagctgc cctgcttcgt tttctcgtat cgcgccgcgt ccaaagtcgt ccagaagcga
      601 ttgtacctcg tggcaggaca tcatgaacgc aaatcctccc gttcggccag caaggttttc
      661 aacatggctt tcgctcgaaa caacctgatt ttcacggttt ccaccgatac ggcaagtgcc
      721 tgtgcagtct cttcgtaaga aaggtcttca aaataccgca acatgatcgg cagccggtat
      781 gattcgggta acgccgccac tgcgttgcgg gtctcctgct cctcgtcgtt cgccaagtcc
      841 gacgccaacc tgttctggtc tggcaacgac gcgaccgggt catcatctga agccggccga
      901 ggaaaacgct tcctcagctt tgcccgggcc gtgcattcgt tcactgtgat gcggtacaac
      961 cacgtcttca gagaacttcc accccggaac aggtgcaggg atcgaagcgc attcaggaag
     1021 acggtctgtg ccgcatcctc ggcttcctgc gtgtcgccca gcatccgtac ggcgagcgtg
     1081 catactgccg actgatagcg ccgcacaagg gcctcggccg ccgcacggtc tccccggcaa
     1141 catgcctcga cgagttcttg atcgctgtgc accggccggc atcccgttgt gcgcgggtgg
     1201 tatgtctgct gttactggat tagagaaaga tgtgcgagaa atgttacaac aggaaggaac
     1261 aaaccgggct tcttgcgacg aggcagacgt gcctgcagcc gcaccggctg ccaccagcat
     1321 gtaccggaac ctttcactgc ttgctgtgtt cggggaaggt tctccacgta agtcggtaaa
     1381 taataagggg aatagcttcg ttttccgccc ggcgatagga ggtttcgacg cggaagcccc
     1441 ggaacgtggc ggcacctgca aaatcccgtg cggtcacccg ttcggggtca acgataatcg
     1501 cggtgccatt ggggagtacg gttctcgcca gcatgtccac catggcagga gtttgccagc
     1561 gttcgtacaa cacatccgcc gcaatcacga catcgaaccg accgagatct ggcggagtgc
     1621 gccagtccac ctcccgcgtg cgcagagata cgccgttcag cgccgcgttt acgtggagca
     1681 ggtccagcgc ctcggggtag taatccgtgg cggtcacctg agcacccaga tgtgcggcga
     1741 cgatgcttgg caggccaagt ccgcagccaa gttccagcac ccgggcatgg cggagtgcgg
     1801 ccgcatccga ggcgaggagt tcggccagcg caacgccact tggccacagc tccgcccagt
     1861 agggcatttt ctcatcctgt ttccatgtgt ccggatctat ttcatccacc agcctgtcga
     1921 ggtcgggcac ccggtagagg tgatacacgt ggtcccggat cgtgcgatgg gtcagaacgg
     1981 cagtctggtt gccgattttc ggcgcgggga aaccggcggg gaccgtgttc atgcgctgct
     2041 cccgccaagc acaaggtccg cgcatgccca cacagagccg cgtggttctt ccccaagcgg
     2101 ttccgacgcc gcctccatca ccagaatcct gttgcgaatc tcacagatcc cgtgccgcga
     2161 catcagtgtc acgcggggag caatgtcgcc gtccaccact gcggacgggc gttcgccgga
     2221 tcggctttcc agagccagag tcaccacgcc tgaaccgcgt gtgcctctga tcgcaagagc
     2281 ggcggattgc gaagagcaga aagcctggaa gcagaatccg gtcgcggaag cctcgaccag
     2341 ccggatgcgg gagaacgggt ggaggcacgc accggaagaa agtgcaggca gacggacgag
     2401 ttccgtgcgg cttccggggg cgaacaaacg caccgggcac acattgttcg ccgtgatcgc
     2461 ttcgaccgtg tagaaaaccg actcgacgcg agcaacgttc accgcccaca aaacggacca
     2521 ttccggggtt gcttcgtcaa cccgttttcg cgtgcgcgga aagaacctca tcgcggttgc
     2581 ggcgtctccc cttccggatt cctcccgtct ttctccggag actgtggggt cgcgggagat
     2641 tccttctcag ggccttcagc ggcttgctcc ttttccttac cccacgaacg ccgccacgcc
     2701 gcctgcttga gcgctcccgc gaacaggacc gcgctaccga ggagcaactg tatccacatc
     2761 cagtgccggt agcgattctg aagggcggtg aaatcggctt tcagcgtttc cacggtggcg
     2821 gcatcgtagg gattctccat gacgcgggag agttcctggc ttccatcctg cacagcgctt
     2881 tccgcggatg cctgaaccgc cagcgatttc ttgagggtgt ccacgctgtt ctgcatcttc
     2941 gtgatgtagg gatcgagatt cccgaatgag tagatcgcga caatgaacat gaacagccct
     3001 gaaagcgatc tgagcaacca gatcacccac cacgcgaagc ttctcctcgc acggcgttca
     3061 aggatgacac cgataacaac actggcaagg aggatgagcg cggcaccgaa gatgtaggga
     3121 cggggccgaa gaatcatttc gaccgcggtt gaggcgccgt tggcgaggag catggggtct
     3181 ccggcggggg agtcatcgag cggaagaccc aggagcaggc tcaccgtcag aaggccgccc
     3241 atccacatcg cgacggcaag gtcggagagc cagtaaaccg cggtaagcac aatggttgcg
     3301 ccgatcatgg catatccctc cctctggcac gaacccctta cgagacagga aggtcgtcca
     3361 gaccgaatga ttcgccccac atacaggtgt gaaccgggtc gaacgggcga atctgcgaca
     3421 gcaggtccgc gaagaacgcc tccccgtacc gagcaacaaa cggtgcagca gaatatacac
     3481 gttcctgcgg aaccccgccc gggaacacaa aactgcttat gcgttccagc tccggcatgg
     3541 tttcccgcac gcgccggcga aatccctgat ccagtttcgc ttcgagtttg ttcatcagcc
     3601 cctgcgtctt gatacgggcc gagagaacgg ctgtatccat ccccggcagt acgttccgaa
     3661 cctcatgttc gagcttctcg tatgtctcgt caagcgcccg ccgggtttcg gcaatgcggc
     3721 cgccgaggct gctgcgcttc tcacgctcag ccagcgtgtg acgccacaca tccagcggtt
     3781 gcaacgcctc ttcgattgca agggagcggc tctgcagcca gcttccgatt tcctgcggca
     3841 ccaccgtaaa acctgcccgc gcggctatca gcggcattgg aacgccagac atgtcgtaaa
     3901 gagcgcgcac ctgggccatg tacgcaattt cgccgggccc catcacgtgc accagcgtcg
     3961 gaaacaccga atcgcctgaa acagggcgga atgctgcgtt cggactgata cgttccggcg
     4021 acacggacgc aatcttctcg aattcgggga tggtgtgcac tgtggcacct tcccgccaga
     4081 agaatccctg cgtcggcgtg aaatccacac gccggcggtt gccggtttcg tcatcccaga
     4141 agaagagcgt tgcctcaggc gaggtgacaa tctgcggaac gtacccctct gtccggacgc
     4201 gtgagctttg ctcgacgaat gcgtcccgaa atcccacggg atccgaactg caccgcgaaa
     4261 aaagtggctg gagaaggcct ttgaatcgtc tgtcggcggg gttggcgaaa atgatcccgt
     4321 agcgacccag ccacgtaacc atgaggcgcg caaacgcatc cacaagggtg gcacccggtg
     4381 cgaaggcttc ccgaatcgcg tcggccgcct ggtcccgacc ctgtccgccg gggagcaacg
     4441 agaccgtttg gtccgcccat cccgtaatct cgccgacgaa acgcaactcc gctacgcggg
     4501 ctgagggcgc ccctgaaacc agcgaacggg gaagttccac atggtgtgga aaacccgtcc
     4561 gcgcgggaaa tgctacgcgc gcaacctctt cgtaatcgtg gtcgtccccg gcgatccaga
     4621 acacgggaac gaccgcctcg ccaagcagag actccaacgc tcgggcccac gctctcgtca
     4681 tcatctccgc gggcttcgc
//
LOCUS       DAOSOO010000233         4680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18350_length_4680_cov_5.886054, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000233 DAOSOO010000000
VERSION     DAOSOO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18350_length_4680_cov_5.886054"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLT86_12810"
     CDS             complement(<1..333)
                     /locus_tag="PLT86_12810"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037053.2"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOT37534.1"
                     /translation="MIVVMHRGATQEEISHVCEHIVELGFKAHPIYGTERVVIGVIGD
                     DHAKTEAMATLGIQPGVEQVTPILKPYKRVGAEMKKERTVVKVGDAAFGGGNFAVIAG
                     PCSVENEEQ"
     gene            complement(352..1095)
                     /gene="hisA"
                     /locus_tag="PLT86_12815"
     CDS             complement(352..1095)
                     /gene="hisA"
                     /locus_tag="PLT86_12815"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT37535.1"
                     /translation="MLILPAIDLRDGRVVRLRQGNPDEQTTYSESPASAAQAWFEAGA
                     EWLHVVDLDGAFAGEPVNIAAIRSIVAAAPVPIEVGGGIRSVGTAAKLLALGVSRVIF
                     GTVAVTHPEIVGEAVERFGAERIVVGLDSKNGNVAVRGWTEASSLAVLAAARHLKESG
                     VVRVIHTDIARDGMFTGPDIDTAALLARETGLSVITSGGVGSLADIRRVAERKELEGV
                     IVGKALYDGAFTLQAALEIARNRSNNATG"
     gene            complement(1200..1940)
                     /locus_tag="PLT86_12820"
     CDS             complement(1200..1940)
                     /locus_tag="PLT86_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT37536.1"
                     /translation="MKGLTQKKRNPSKEIDLIAFVAHELKNPLASCLMNAYMLKDGLL
                     GELNERQQKAVASLVRNLEYFDDTIHHYLNLTRIEQGNLKIEPRSILVVHDVVQPCLE
                     NLDRQITTKKIKVVNELPPGDIRLVADPALLRIVFNNLLNNAIVYGREGGTVRLSAFE
                     HGGWWEFSVWNEGVGIPKNKIKTLFRKFNRVENPSAPKLRGTGLGLYITRTIIERHGG
                     TISADSEEGSWAQFRFTLPAVVSPASGA"
     gene            2283..>2740
                     /locus_tag="PLT86_12825"
     CDS             2283..>2740
                     /locus_tag="PLT86_12825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOT37537.1"
                     /translation="MAQALDNIRVDEKGEKMEKRKRGCTRAILALVALVTFGGCAVSV
                     NPVTGKRGMYAYSWQQEISLGAETDVAIGQEYGLYEDAALTSYVRSVGESVLAKSHLR
                     RPEAREEFRKTVFTFRVLDSPVVNAFAVPGGYVYVTRGLLAYAENEAQLAV"
     assembly_gap    2741..2840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2841..3668
                     /locus_tag="PLT86_12830"
     CDS             <2841..3668
                     /locus_tag="PLT86_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HOT37538.1"
                     /translation="RSFFHSLTRMSEQSGGRIPTFMSTHPDPGDRENTVVRLAAEWAA
                     QGYATNKVGRDTLFDHIDGMILGQNPRQGFTDGGVFYHPDLRFQFPVPNLWRTVNDAA
                     QVVLVAPDQNANIQISIVPGATTASAAAASFLSQDGVTATENTSVTVNGLPARDVRAA
                     VTVSGTALSVVAYFIEYNGKVYAFVGLTPKALAATYESSFLNTMRGFRALTNSTILAV
                     KPFRVKTVVASRTGAFSSFLPATMPPGFTANDAAIMNQVELTDTIQKGTRLKLWEQR"
     gene            3676..4323
                     /locus_tag="PLT86_12835"
     CDS             3676..4323
                     /locus_tag="PLT86_12835"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT37539.1"
                     /translation="MGGAGEILRRAAARYRDALAIVDDYMERETARRREQVHCRRGCA
                     ECCFGTFAFSLADMALVQSAFGSLNEEIQEYCVRRATAFVENMGEVAARRVDLLDDAE
                     LDRLLEPVAALPCPFLSPEHDCRIYRSRPLVCRIHGLPIKGYGNELLDPGCRLNFVGI
                     RVEELSGYQLDIAEFDVAEATEIAGIAEALGVDEETQILLPAAILPPWFLPSDEK"
     gene            4426..>4680
                     /locus_tag="PLT86_12840"
     CDS             4426..>4680
                     /locus_tag="PLT86_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37540.1"
                     /translation="MPRTVTNSHALRRLALSLANWSEAPPRVLLRRGDSVRFAHAIAL
                     LGLQLMQTASESETSPTGSSRESSVLVRDGFAERVQTMVSL"
BASE COUNT          900 a         1309 c         1384 g          987 t
ORIGIN      
        1 ttgttcctcg ttttctacgg agcagggacc ggcgatcacc gcgaaatttc cacccccgaa
       61 tgcggcgtcc ccgactttca ccaccgtcct ttcttttttc atttccgcgc cgacgcgttt
      121 gtacggcttg aggatcgggg tgacctgttc cacgccgggt tgaatcccaa gcgttgccat
      181 cgcttcggtt ttcgcgtgat cgtcgccgat cacgccgatt accactcgtt ccgtgccgta
      241 gatcgggtgg gccttgaacc cgagttccac gatatgctcg catacgtggc tgatttcttc
      301 ctgtgttgca ccgcggtgca tgacaacgat catgcgtacc cctcagtcaa ttcatcccgt
      361 tgcgttgttc gacctgtttc gtgcaatttc cagcgccgcc tgcagggtaa atgctccgtc
      421 gtacagcgct tttcccacga tcactccctc cagctctttc ctttcggcaa cgcggcggat
      481 gtcggccagc gagcccacgc cgccggaggt gatcacggac agaccggttt ctctcgcaag
      541 cagggcagcg gtgtcgatat cgggtccggt gaacattccg tcacgggcga tatcggtatg
      601 gatgacccgg acgactcccg attctttcag gtgtcgtgcc gccgccagaa ccgcaagaga
      661 gctcgcctcg gtccaacctc gcaccgcaac atttccgttc ttggagtcca gacccaccac
      721 gatccgttcg gcaccgaacc gctcaactgc ttcgccaacg atttccgggt gagtgacggc
      781 gacggttccg aaaataaccc gtgaaacgcc aagcgccagc aatttcgccg cagtaccgac
      841 ggagcgtatg cccccgccga cttcaatcgg aacaggcgcc gcggcgacga tcgagcggat
      901 tgctgcgatg ttcaccggtt cgcctgcgaa tgcgccgtcc agatccacca catgcagcca
      961 ctccgcacct gcctcgaacc aggcctgtgc cgcacttgcg gggctctcgg aatacgttgt
     1021 ctgttcgtca gggttgccct gtcgcaggcg tactacccgt ccatcacgca aatcaatcgc
     1081 aggcagaatc agcatcggag caagggaagg attgtggaaa atcgccgaac gtcaattgca
     1141 cgcgaggcga ggggaaaata taacggacgg ggcgcaactc gtccaccctt ggcgctcgat
     1201 caggcccccg aagccggtga aacaaccgcg ggcaatgtga atcggaactg ggcccaggaa
     1261 ccctcctctg agtctgcgga gatggttccg ccgtgccgtt cgatgatcgt gcgcgtgatg
     1321 tagaggccga gaccggtgcc gcgaagtttc ggagcggaag ggttttccac ccggttgaat
     1381 ttgcggaaaa gggtcttgat tttgttcttg ggaatcccca cgccttcgtt ccacacagaa
     1441 aactcccacc atccgccgtg ttcgaatgcg gacaggcgga cggttccccc ctcgcgcccg
     1501 tacacaatcg cgttgttgag gagattgttg aacacgatgc gcagcagggc tggatccgcg
     1561 accagacgga tatccccggg cggcagttcg ttcacgactt tgatcttctt cgtggtgatc
     1621 tgcctgtcca ggttttccag acacggttga acgacgtcgt gaactacgag gatggaacgc
     1681 ggttctattt tcaaattgcc ctgttcgatc cgcgtaaggt tgaggtaatg atgaatcgtg
     1741 tcatcgaaat attcaagatt cctgacaagg gaagcgaccg ctttctgctg ccgttcgttc
     1801 agttccccca gcagcccgtc tttcagcatg tatgcgttca tcagacagga ggcaagaggg
     1861 tttttcagtt cgtgcgcaac gaacgcaatg aggtcgattt ccttggaagg attgcgcttt
     1921 ttctgagtca aacccttcaa ctgttgttcc ttgtctgggc acagtgcgcc atttctgcgc
     1981 aggccttttt ccgcccgaag caggcatcat gagaacagga gagccgccgc gggcggagaa
     2041 gcgtgacctc gcaccgtcgg gaacgccgca gcgctttttc agaactcccg acccttcaga
     2101 atagacaggt gcaaattccg cgccggttgg tgtgttcgac attgccgcaa gagacgatta
     2161 cctgcgccgc cgaaaaggaa gcccgattac ggtcgttttg ttcgaaggcg cacgcaggca
     2221 gacacaaaat cttgtggcgc ccgatgtatt tgaagcgcga atccggctac atttgttagg
     2281 aaatggcgca ggcgttggac aacatacgcg tggacgaaaa gggcgagaaa atggaaaagc
     2341 ggaaacgggg atgcaccaga gcaattcttg cattggttgc tcttgtcacg ttcggtggct
     2401 gtgcggtttc tgtcaacccg gttaccggaa aacggggcat gtacgcgtac tcctggcaac
     2461 aggagatcag cctgggtgcg gaaaccgacg tagcaattgg ccaggagtac gggctttacg
     2521 aggatgcggc gctcacgtcg tacgtgcgga gcgtcggcga gtcggttctt gcgaaaagcc
     2581 atctccggcg tccggaggcg agggaggagt tcaggaaaac ggtgttcacc ttccgggtgc
     2641 tggacagccc ggtcgtaaac gcctttgcag ttcccggagg ttatgtgtac gtaacgcggg
     2701 gactccttgc gtatgcggag aacgaggcgc agcttgccgt nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn cggtcgtttt ttcacagtct gacgcggatg agcgagcaat
     2881 ccggcggacg gataccgacg ttcatgtcaa cacatccaga cccgggggat cgcgaaaaca
     2941 cggttgtcag gctggcagcg gaatgggcgg cacagggtta cgctacgaac aaagtgggca
     3001 gggacacgct ttttgaccac attgatggga tgattctggg tcagaaccca cgccagggtt
     3061 tcacagacgg aggtgtgttc taccatccgg acctccgttt ccagttcccg gttccgaact
     3121 tgtggcgaac cgtgaatgac gcggcgcaag tggtgcttgt cgcaccggat cagaacgcca
     3181 acatccagat ttccattgtt ccgggtgcga cgacggcttc ggccgctgcg gcctcctttc
     3241 tttcccagga cggcgtaact gcaacggaaa acacgagtgt tacggtgaac ggtctccctg
     3301 cgcgggatgt gcgggccgcg gttaccgtga gcgggactgc gctgtcggtg gtggcgtatt
     3361 tcatcgaata caacgggaag gtatatgcgt ttgtcggact cacgcccaag gcgctggccg
     3421 cgacgtacga gtcgagcttc ctgaacacga tgcggggttt ccgcgcgctc acaaattcca
     3481 cgatccttgc cgtgaagccg ttccgggtga aaacagttgt ggcaagccga acgggtgcgt
     3541 tttcctcgtt tcttcccgcc acgatgcccc cggggttcac ggcgaacgat gcggcgatca
     3601 tgaatcaggt ggagcttacg gataccattc agaagggtac ccggctcaaa ctgtgggagc
     3661 agcgttagtt tgccggtggg tggtgccggg gagattctgc ggcgggcggc agcgaggtac
     3721 cgggatgccc ttgccatcgt cgatgactac atggagcgtg aaaccgcgcg ccgccgtgag
     3781 caggttcatt gccggcgtgg ttgcgcggaa tgctgcttcg ggacgtttgc cttttcactc
     3841 gccgacatgg ctctcgtgca gtccgcgttc gggagtctga acgaggaaat tcaggagtat
     3901 tgtgttcggc gcgcgaccgc gttcgttgaa aacatgggtg aggttgcggc gcgtcgggtt
     3961 gatctcctgg atgatgcaga gttggaccgg cttcttgaac ccgtggcggc gttgccgtgc
     4021 ccttttctga gcccggaaca cgactgccgc atttaccgat cgcgccctct ggtatgcagg
     4081 atccacggcc tgcccataaa aggatacggt aacgagcttc ttgaccccgg gtgtcggctc
     4141 aatttcgtgg gaatacgtgt cgaggaactg agcgggtatc agcttgacat cgccgaattc
     4201 gacgttgcgg aagcgactga gatcgcgggg atcgccgaag cgctcggtgt tgacgaggag
     4261 acacagatcc ttctcccggc ggctattctc ccgccctggt ttctgccgtc tgatgaaaaa
     4321 tgagaatgga gaacggcaaa gcgcggcacg tctcgcgttt ccggaaatcc gaaccgcatc
     4381 cagattgatc ccgcggagac cgcaccagaa gaacgtgtgt tcgcgatgcc ccgtactgtg
     4441 acgaattccc acgctttgag gcggctggct ctctcacttg ccaactggag cgaagccccg
     4501 ccgcgcgtgc ttctgagaag aggggattcc gtccgtttcg cccacgcgat cgcactcctc
     4561 gggctccagc tcatgcagac agcgagcgaa tcggaaacct caccaaccgg gtcttcccgg
     4621 gagtcctcgg tgttggtgag ggacggcttc gcggaacgcg tgcagacgat ggtatcgctt
//
LOCUS       DAOSOO010000234         4618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18691_length_4618_cov_4.743151, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000234 DAOSOO010000000
VERSION     DAOSOO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18691_length_4618_cov_4.743151"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..2690
                     /locus_tag="PLT86_12845"
     CDS             30..2690
                     /locus_tag="PLT86_12845"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOT37541.1"
                     /translation="MTENTLPDVATGRPDRRVLRGEALRAVAMPLGGIGAGQIALAGD
                     GGWRQWQIMNIPNHLGHVPHSFFAVRMRPHAVHFTERRSPEDVMGVSRVLMSSALYDS
                     PDFIPPVTSSDHIVPEESRTLLRQLPGVDEVRYVGEYPVAEVIFSDPSLPLPVTLEAY
                     SPFAPLDEEFSGLPAILCDLVVTNTLESPVHVSLAGTLQNAVGWDGKSRISGTRNPGY
                     GGNENRVVRARGLTCIEMTNPSVPAESNRFGQMALSLLGGNVTWLGRWDTLEAFWADF
                     ADDGDLSCTDDAAPTSPGSTANGALCRSAWLKPGETLKATFVISWFFPNHYVNWDQSG
                     FGLKDRKSRFHIGTHYATRFQSALDVAMYVSDHAEVLRKRTFDFRNSFYDSSLPYSVL
                     DAVSSQASIIRSPTCMWLEDGTFHAFEGCHGASTGSAEDVGGCCPLNCNHVWNYEQAL
                     ARLFPRLEQAMRRTDLLHQMTEEGRIGHRTLLPFYLPRWTEPATDGQLGTVLKLYREY
                     RACGDRAFLDELYPKAKKALEWVFTKHDVDGKGILDGFQPNTYDCALHGHNTFVTGLY
                     LAALRAAEEMAKLLGDSSFGEECRARFESGKVAIDAECWNGEYYIQRPEPGKSMDMQY
                     GEGCHIDQLFGQWWAHILDLGYVFPSDHVREALRSILRYNWRDSFSGFRQSPRVFASD
                     HDSGLLICTWPHGGRPDKPTLYSDEVWPGTEYEIAGLCLFEGFAEEAFKVVEGLRKRY
                     DGVQRNPWNEVECGDHYARSMASWTVLEAACGMRYDAGRSFLGFGPNVTPERFRAPFV
                     TASGWGVFSQRITDQMATAEVIVSHGTIPLACFELDMPSKAVVVGKVLVNGKPVRCTC
                     STLDRRAHIAFGSLLELREGNTLTADVKTA"
     gene            complement(2758..4137)
                     /locus_tag="PLT86_12850"
     CDS             complement(2758..4137)
                     /locus_tag="PLT86_12850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT37542.1"
                     /translation="MAGHRDAEQKALQSSMKSVTGFKGKGRPKIGVEEFMSIAERFGL
                     SKPALEKIQSILAEEDLGAGPFLANYYSGLPESRVQAFQRVARETFGSRYAIGLSSGT
                     GALHAAFVAVGVGPGTEVICPAIGFFATAAAVVMAKGVPVFCDVDESLHMDPAKIEAL
                     ITPRTVALAPTHVMGSVCNMPAIMAVARKHNLKVVEDCAQSCGGKVNGSYVGTFGDVG
                     IFSISAYKIVGGGEGGLLITDDERLWDRANCLSEGGGLWRPERFAPPRYEGELFCGTN
                     YRMSELEAAVDVVQLSKMPETVARFRTVKRQITGRLKTYREIRPQTMNDPDGEVGYTL
                     RFFPETIELGQEIAKALKDAGIGAGIRGRNGRPDWHIYHDMYPITLKTKSGADDCSFH
                     CPHYAAKGGKIEYRLGDCPVADDLFDRVVNVGLNQWYTEEDCVQFADVMNSVLSHFCT
                     EDPSAKPWR"
     gene            4296..>4618
                     /locus_tag="PLT86_12855"
     CDS             4296..>4618
                     /locus_tag="PLT86_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT37543.1"
                     /translation="MPRFDVVGFGIVTIDHIGLLDHYPAEDTKQRLRMYVQQGGGTVG
                     TPLVACARLGLNAAYIGRIGDGPSSHAVLDDFRRERVDTTGVVSVKGYEPPVGLILVN
                     PATGSR"
BASE COUNT          913 a         1352 c         1394 g          959 t
ORIGIN      
        1 gatcatcggt ctggaccgga ggaaacacga tgactgaaaa cacgttgccg gatgtggcga
       61 ccggcagacc cgaccgtcgc gtgcttcgcg gcgaggcgtt gagagcagtc gccatgccgc
      121 tgggcgggat cggcgcaggt cagattgctc tggctggcga cggtgggtgg cggcaatggc
      181 agataatgaa cataccgaac catctggggc acgttccgca ttcgttcttt gccgtgcgca
      241 tgcgtcccca cgcagtgcat ttcacggaac gccgaagccc ggaggacgtg atgggtgtct
      301 cacgggtgct catgtcttcc gcgttgtacg acagcccgga tttcattccc cccgtgacaa
      361 gttccgacca catcgtgccg gaggaatcac ggactcttct ccggcaactg ccgggtgttg
      421 acgaggttcg ttacgtcggc gagtaccccg ttgcagaggt gattttctcc gacccgtcgc
      481 tgcccctccc tgtcaccctc gaggcgtatt ccccctttgc gcctctcgat gaggaatttt
      541 ccggccttcc ggcgatcctg tgtgatctgg tggtaaccaa tacgctggaa tcgccagtgc
      601 atgtcagcct ggcggggact ctccagaacg ccgtggggtg ggacggcaag agccggattt
      661 ccggaacacg gaaccccggg tacggcggaa atgaaaaccg ggtcgtacga gcgcggggac
      721 tgacgtgcat cgagatgacc aaccccagcg tcccggctga gagcaaccgg ttcgggcaga
      781 tggcgctttc gctactgggc ggcaatgtga cgtggctggg cagatgggac acgttagagg
      841 cgttctgggc ggattttgcc gacgatggtg acttgagctg tacggacgac gctgccccga
      901 cttcccctgg atccactgcc aacggcgcat tgtgtcgctc cgcgtggctg aagccagggg
      961 aaaccctgaa ggcgacgttt gtcatctcct ggttctttcc gaatcattac gtcaactggg
     1021 atcagagcgg tttcgggctg aaggaccgaa aaagccggtt ccacattggc actcattacg
     1081 cgacgcgttt ccagagcgcg ctcgatgtcg ccatgtacgt gagcgaccac gcggaagtgc
     1141 tccggaaacg gacgtttgat tttcgcaatt cattctacga cagttcatta ccgtattccg
     1201 tcctcgatgc tgtttcgtct caggcaagca tcattcgcag ccccacctgc atgtggctgg
     1261 aagacggaac cttccatgcg ttcgaaggat gccacggcgc aagcacgggt tccgccgaag
     1321 atgttggtgg atgttgcccc ctcaattgca atcatgtctg gaattacgaa caggcccttg
     1381 cccggctttt cccgcgtctg gagcaggcca tgcgccgtac ggaccttctc caccagatga
     1441 ccgaagaggg gcgaatcggc cacaggacgc ttttgccgtt ttacctgccg cgatggaccg
     1501 aaccagcgac agacgggcaa ctcggtactg tgctgaagct gtaccgcgaa taccgagcct
     1561 gcggcgatcg cgcgtttctg gatgagctgt atccgaaggc gaaaaaggcg ttggagtggg
     1621 ttttcacgaa gcacgatgtc gatgggaaag gcattctcga cgggttccag ccgaatacgt
     1681 acgactgcgc actgcatggc cacaacacgt ttgtcaccgg tctatatctg gccgcattgc
     1741 gggccgcaga agagatggcg aagctgcttg gagacagctc tttcggtgag gagtgccgcg
     1801 cccggtttga atcagggaag gtcgcgattg atgccgaatg ctggaacggg gagtactaca
     1861 tccagcgtcc ggaacccggg aaaagcatgg acatgcaata tggcgaggga tgccacattg
     1921 accagttgtt tggacaatgg tgggcgcaca ttcttgatct cggctacgta tttcccagcg
     1981 accatgtccg cgaggcgttg cgctccatcc tgcgttacaa ctggcgggac agcttttccg
     2041 ggttccggca gagcccgcgg gtgtttgcct cagaccatga cagcgggctg ctgatctgca
     2101 cctggccaca cggaggacga cccgacaaac caacgctgta ctcggacgag gtgtggccag
     2161 ggacggaata cgagatcgcg ggcctctgtc tgttcgaggg tttcgccgaa gaagccttca
     2221 aagtggtgga ggggctgagg aaacgttacg acggggtgca gaggaatccg tggaacgaag
     2281 tcgagtgcgg tgatcattac gcgcgcagca tggcgagctg gacggtgctc gaggcagcgt
     2341 gtggcatgcg ttacgatgcg ggcagaagtt tcctcgggtt cgggccgaac gttacgccgg
     2401 agcgtttccg cgcacctttc gtcacggcgt caggatgggg agtgttcagt caacggataa
     2461 ctgaccaaat ggctacggct gaggtgattg tctcgcacgg aacgataccc ctcgcgtgct
     2521 tcgaactgga catgccttca aaggccgtgg ttgtcggtaa ggtgctggtt aatggcaaac
     2581 ccgtgcgatg cacctgctcc actctggaca ggcgagcaca catagccttt gggtcgcttc
     2641 tggagttgcg cgagggcaat acgcttacgg cggatgtcaa aacggcgtag tgacacggag
     2701 ggcacaaaac cggagggcgg acgactaccc gccctcccgg ttctcccata gggcacttca
     2761 gcgccagggc tttgccgagg gatcctccgt gcagaaatgt gaaagcacgc tgttcataac
     2821 gtcggcgaac tgaacgcagt cctcttcagt gtaccactgg ttcaaaccca cattcaccac
     2881 gcggtcgaaa aggtcgtccg ctacggggca atctccgagc cggtactcaa ttttcccgcc
     2941 ctttgccgca taatgggggc aatggaagga acagtcgtcg gcgccgcttt tggttttcag
     3001 cgtgatggga tacatgtcgt gatagatgtg ccagtccggc cgcccgttcc gtccccggat
     3061 accggcgccg attccggcgt ctttgagggc cttcgcaatt tcctgcccca gttcgattgt
     3121 ttcggggaag aaacggagcg tgtagccgac ctcaccgtcg ggatcgttca tcgtctgcgg
     3181 acgaatttcc cggtacgttt tcagtctgcc ggtgatctgg cgtttcacgg tccggaaacg
     3241 cgccaccgtt tccggcatct tcgatagttg cacgacatcc accgcagcct caagctcgga
     3301 catgcggtag ttggtgccac agaacagctc tccctcatac cgtggaggcg cgaaacgttc
     3361 cggacgccac aatccgccgc cctctgagag acagttggca cgatcccaga ggcgctcgtc
     3421 gtcggtgata agcagccctc cctctcctcc gcccacgatc ttgtatgcag agatactaaa
     3481 gattcctacg tcaccgaaag tgcccacgta gcttccgttc actttgccgc cacaggattg
     3541 ggcgcagtct tccaccacct tcaggttgtg cttacgcgca actgccatga ttgcgggcat
     3601 gttacacacg ctccccatca cgtgggtcgg tgcgagggca acggtgcgcg gggtgataag
     3661 cgcctcgatc ttggccgggt ccatgtgaag cgactcgtct acgtcgcaga acaccggcac
     3721 gcccttcgcc atcaccaccg ctgctgcagt ggcgaaaaaa ccgatcgcag ggcatatcac
     3781 ttctgttccc ggaccaacgc cgaccgcgac aaaggccgca tggagcgccc ctgtccccga
     3841 actgagaccg atcgcgtaac gagagccgaa tgtttccctc gcaacgcgct ggaacgcctg
     3901 cacacgcgac tctggaaggc cgctgtaata gttggcaaga aagggaccag ctcccaggtc
     3961 ctcttccgcg agaatcgact ggatcttttc caacgccggt ttgctcaagc caaaccgttc
     4021 cgcgatggac atgaactcct cgacaccgat tttcggtctc cctttacctt tgaacccggt
     4081 gaccgatttc atactggatt gcaacgcttt ctgttccgca tcgcgatggc ctgccattga
     4141 tcattctccc cggcgagatg gattggttga accgcggaca tacgcccgat ggaatgtacc
     4201 tccgcacggg cgcgagggca tacgggccgt ggcggaagcg ctgtgccgaa gcgtacattt
     4261 tcttcctctt ttcgccagac aagggatgag aagccatgcc gcgttttgac gtcgtcggtt
     4321 ttgggattgt gacgattgac cacatcggcc ttcttgacca ctaccctgcc gaggatacca
     4381 aacagcgcct ccgaatgtat gtgcaacagg gcggcggcac ggtaggtaca cccctcgttg
     4441 cgtgcgcaag gttgggtctc aacgccgcgt atatcggccg catcggcgac ggcccgtcaa
     4501 gtcacgcagt gttggatgac ttccgccgcg aacgggtgga caccaccggg gtagtttccg
     4561 tgaaaggata tgagccaccg gtgggtctca ttctcgtcaa ccccgccacc ggcagtcg
//
LOCUS       DAOSOO010000235         4590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18865_length_4590_cov_4.851158, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000235 DAOSOO010000000
VERSION     DAOSOO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18865_length_4590_cov_4.851158"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLT86_12860"
     CDS             <1..1475
                     /locus_tag="PLT86_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37544.1"
                     /translation="FFAFLVLCGSLTAVLRKQWFDHEKIRFPQAEVTLALVEGSGGAS
                     GRNTVSGSPPFWVGFALSAGILVWNSVSYFRPIWPRIDFSQMAPWVLNFGPGFGVVDL
                     VPDPFVIGISYLVDVKILFSVWFFFLLGRVQQAVQVKTGYVSGQTDLWITSDNLSGWQ
                     SLGGLFFVVFWFLWMSRAHFKRVLQHVLHPETSQEDDSNEMLSYRMSLILLFACSVYM
                     VLWLIHLGLDWYVALVFFVALVVLVLGITRIVAETGMPLVGSPVTAQGITTRLFGDAA
                     LGPSNIAGLAVTYAAFRMVEGYPMPVTMHAARLGDSAGASRRTLFGAVLFGSVAAMVV
                     MSVVTIALAYNGGAFNFGAHHAFHQMYEAYDHMTSRIRSPYPWDWRLFAHFGGGALFV
                     SLLTFLRYRFGWDSLNPIGFCTATTFYHSAIWSGVFITWATKIIVHKIGGLSLYRRCY
                     PFFIGLVAGHVAGAIFCLAVDWIWFPGAGHDIRTMIYFFG"
     gene            1598..2467
                     /locus_tag="PLT86_12865"
     CDS             1598..2467
                     /locus_tag="PLT86_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT37545.1"
                     /translation="MYASIRDDTVQSAYPDIITGLKALGLSAIEANFTRQVSLPSLTD
                     KTVFFPVTTSAEIAEYKKHLDQYNVKISAFLLANNLNADDLEDELAWCTKALKVAHAL
                     GVKAVRIDSAMKGQQELPFDVRVEKFVKGIRALIDATPDAPVDMGIENHGYQGNDPNF
                     LHGVFDGVGSSRIGMTLDTGNFYWRGHALEKTYAVIKEFAPLAKHTHIKNINYPEDKR
                     NITREMGWEYGKYVCPIPDGDVDHTIVVGYLRDAGYDRDLCIEDESLGKFPIEERRNV
                     LLRDAEYIKGLAK"
     gene            complement(2574..3146)
                     /locus_tag="PLT86_12870"
     CDS             complement(2574..3146)
                     /locus_tag="PLT86_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT37546.1"
                     /translation="MSVWVSRGGDKLSAAFQAWKSVLPRLEGAFAADFGSNVGGFVQC
                     LLAQGITRVYAIDTGYGVLAWELRNDPRVCVMERTNALHVVLPEKVDLVTIDVAWTKQ
                     RLILPAAFRLLRDSGLVVTLVKPQYEAAQKERRGGVLPGERVPDVLMRVRAEVRSVGG
                     EILAELESPIKGAGGNTEFLWLVRPGNATF"
     gene            complement(3143..3601)
                     /locus_tag="PLT86_12875"
     CDS             complement(3143..3601)
                     /locus_tag="PLT86_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37547.1"
                     /translation="MRGFCSHVVIAAAATAVFFSWLCGCGILSSTGTQNPLWGVWSGP
                     YPSSLGTQADSSRFAFTSKRVYLFFALKADGTILAREFGYYSSNGYLMILSSDPGGNL
                     DREEIFYEFVGESLVATIRGQRVVYRRVGGVEWADDLGSAAHLKEGAVGR"
     gene            complement(3633..4166)
                     /locus_tag="PLT86_12880"
     CDS             complement(3633..4166)
                     /locus_tag="PLT86_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37548.1"
                     /translation="MKRLWLFLILAPHVACAGGLAGLGFTANVPRQTLGGVIVYSWTY
                     ADVKVSRNFLGIWRDTYESISVPEAVYEFGDSLINSARTYKTVDAGLQARLSHRTTAY
                     LGLGVTETRHLYQFLDDFRILGRNGQYWVEHEVDYGINVNGGVYLRVAGPLYGQIGFN
                     TNPRGLDLGVAIIFASP"
     gene            complement(4240..>4590)
                     /locus_tag="PLT86_12885"
     CDS             complement(4240..>4590)
                     /locus_tag="PLT86_12885"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOT37549.1"
                     /translation="PLNTLTDVFVLNRWGHFKPLRDWNAEGVKHAVVVDGDPSDPLSE
                     DRSWTMECAIPFRELVTAPNIPPKSGDSWRGNFYRIEQGYEWQEYTAWSPTGEVNFHH
                     PERFGTIVFTSETA"
BASE COUNT          987 a         1363 c         1286 g          954 t
ORIGIN      
        1 ttttcttcgc atttctcgtg ctctgcggat cgttaaccgc cgttctgcgg aaacaatggt
       61 tcgaccacga gaagattcgc ttcccgcagg cggaagttac ccttgcgctc gtggaggggt
      121 cagggggcgc atcgggccga aacaccgtat ccggcagccc cccattctgg gtcggttttg
      181 cattgtcggc gggcatcctc gtatggaaca gcgtgagtta cttccgccct atctggccgc
      241 gaatagattt ttcgcagatg gcgccgtggg tgctgaattt cgggccagga ttcggcgtag
      301 tagacctcgt tccggatccc tttgtgatag gaatttctta cctcgttgat gtcaagattc
      361 ttttcagcgt atggttcttc tttcttctcg gacgtgtgca acaggcggtc caggtgaaaa
      421 ccgggtatgt cagcgggcag actgacctgt ggatcacgag cgataatctg agcgggtggc
      481 agagcctggg tgggcttttc tttgtggttt tctggttcct gtggatgtcg cgggcgcatt
      541 tcaaacgcgt tctgcagcac gtgttgcacc cggagacgag ccaggaggac gattcaaatg
      601 aaatgctctc gtatcggatg tcattgatcc tgctcttcgc gtgcagcgtc tacatggtgc
      661 tgtggctcat ccatctcggc ctggactggt acgtcgccct cgtctttttc gttgcacttg
      721 tggtccttgt cctcggaatt acccggattg tcgcggaaac cggaatgccg ctggtgggaa
      781 gcccggtgac agctcagggg atcacgacca ggcttttcgg ggatgcagcc ctcggcccat
      841 ccaacattgc cggcctcgcg gttacttacg cggccttccg tatggtggaa gggtacccga
      901 tgcctgtgac aatgcatgcc gctcgtctcg gagacagcgc cggcgcctca cgcaggacgt
      961 tgtttggcgc ggtgcttttt ggctccgtcg cggcgatggt ggtaatgagc gtggttacga
     1021 tcgccctggc gtacaacggc ggcgcgttca atttcggcgc gcatcacgcg ttccaccaga
     1081 tgtacgaagc ttatgaccac atgacgagca ggattcgctc gccctacccg tgggattggc
     1141 gcctgttcgc tcattttggc ggcggagcat tgttcgtcag tctgctcacg tttctgcggt
     1201 atcgcttcgg gtgggattca ctcaacccga tcggattctg cacggcaacc acgttctacc
     1261 acagtgcgat ctggtcgggt gtattcatca catgggctac caaaatcatc gtccacaaaa
     1321 tcggcggtct gagtctgtac cgccggtgtt acccgttttt catcggcctt gtggccggac
     1381 acgttgcagg ggcgattttc tgcctcgcag tggactggat ctggttcccc ggcgcagggc
     1441 atgacatccg cacgatgatc tacttcttcg ggtagcctcc cgggcaccag gcattgcatc
     1501 gctctgccgc ggtgacgaat cttttatcaa gcatccgcgg aacgggccgt atctcgaacc
     1561 ggcccgacct tcaccatctt cacaaaggcg gcataccatg tatgcatcaa tcagagacga
     1621 tacggttcag tcggcgtatc ccgacatcat cacaggcctg aaggcgctcg gtctttcggc
     1681 gatcgaagcg aacttcacga gacaggtatc gcttccgtcg ctcacggaca agaccgtgtt
     1741 tttccccgtg acgacaagcg ccgaaatcgc ggaatacaag aagcacctcg atcagtacaa
     1801 cgtgaagatc agcgcgtttc ttctggcaaa caatctcaac gcggacgacc ttgaagacga
     1861 actcgcatgg tgcacgaagg cgctcaaagt tgcacatgcg ctcggcgtca aggcggttcg
     1921 gattgacagc gccatgaaag gacaacagga gcttcccttc gacgtgcgcg tggagaagtt
     1981 cgtcaaaggg atacgcgcgc tcatcgatgc caccccggat gcaccggttg acatgggaat
     2041 tgagaaccat ggctatcagg gaaacgaccc gaacttcctg cacggcgtgt tcgatggagt
     2101 ggggtcgagc cgcataggta tgacgctcga taccgggaat ttctactggc gcggccacgc
     2161 gttggaaaag acgtacgcgg tcatcaaaga attcgccccg ctggcgaaac acacccacat
     2221 caagaacatc aactaccctg aagacaagcg aaacatcacc cgcgaaatgg ggtgggaata
     2281 cggcaagtac gtctgcccga taccggacgg cgacgtcgat cacaccatcg ttgtcggcta
     2341 cctgagggat gccgggtacg atcgtgatct ctgcatcgag gacgaatctc tggggaaatt
     2401 tccgattgag gaaagaagga acgtccttct tcgcgacgcg gagtacatca aggggctcgc
     2461 aaagtagggt gacacagagc gatcacgcgt cctgagaaag ggggacgtca accgacgatg
     2521 tccccctttc catttgcccg cgccagaagg gaaccggccg gtcaacgacg cgcttagaac
     2581 gtggcgtttc ctggacgaac cagccagagg aactccgtgt tccctcccgc tcctttgatg
     2641 ggtgactcca gttccgcgag gatttctccg cccacggaac gaacttctgc ccgcacccgc
     2701 atgagaacgt ccggcacgcg ttcgcccggc agaactccac cccgcctttc cttctgagcc
     2761 gcctcgtact gcggcttgac cagcgtaacg acaagcccac tgtctctcag aagtcggaac
     2821 gccgcgggga gaataagccg ttgtttcgtc catgcgacgt cgatcgtgac gaggtcaacc
     2881 ttttctggaa gcacaacatg cagcgcattt gtccgctcca tgacgcacac tcgagggtcg
     2941 ttgcgcagtt cccacgcgag aacaccgtat ccggtgtcga tggcatagac tcgagtgatc
     3001 ccttgcgcaa gaaggcattg cacaaatcca ccgacattgg aaccgaaatc cgccgcaaaa
     3061 gcaccctcaa gccgaggaag aaccgacttc caggcctgga atgcggcgga aagtttgtca
     3121 ccgcctcgac tcacccacac actcaccgcc cgaccgcacc ctccttgaga tgagctgcag
     3181 atcccagatc atccgcccac tccacgcctc ccacgcgccg gtacaccact cgttgtccgc
     3241 ggatggtcgc cacgagcgac tccccgacaa actcatagaa aatctcttcc cggtcgagat
     3301 ttccgcccgg gtccgaggaa agaatcatca ggtacccgtt tgagctgtag tagccgaatt
     3361 ctcgggcgag gatagtaccg tccgctttca gcgcgaaaaa caggtacacc cgtttcgacg
     3421 tgaacgcgaa gcgtgagctg tctgcctgcg taccgagaga agaggggtaa ggtccggacc
     3481 agacacccca taaagggttc tgcgtaccgg tggacgagag aatgccacag ccacaaagcc
     3541 acgagaagaa caccgccgtg gcagccgctg caattacgac gtgcgaacaa aacccgcgca
     3601 taccattgcc cgaatgcgcc ggggtgactc cctcacgggg atgcaaagat gatggccacg
     3661 ccaaggtcaa gcccgcgagg gttcgtattg aaaccaatct ggccatacaa cggcccggca
     3721 acgcggaggt acacccctcc gttcacgttg ataccgtagt ccacttcgtg ctcgacccag
     3781 tactgcccgt ttctccccaa tatgcgaaaa tcgtccagaa actggtaaag gtggcgtgtt
     3841 tccgttacgc caagacccag gtaagcggtg gtacgatgcg acaaccgcgc ctgcagaccc
     3901 gcgtcgacag ttttgtaggt acgggcggag ttgatgaggc tgtcaccgaa ctcatacacc
     3961 gcctccggaa cactgatact ctcgtacgta tcgcgccaga taccgagaaa attccgcgat
     4021 accttcacgt cggcataggt ccacgaatat acgatcacac ccccgagggt ctgcctcggg
     4081 acattagctg tgaatcccag cccggcaagt cctcccgcgc atgccacgtg gggtgccaga
     4141 atgagaaaaa gccaaaggcg tttcatgaaa acaggattcc ggggcaaccc gggcacgcgc
     4201 catggttgcg caatcagcgc ccgggcgatc ctctcgtgcc tatgcagtct cagaggtgaa
     4261 cacgatcgtc ccgaaacgct ccggatggtg gaaatttacc tcgcccgtgg gagaccatgc
     4321 cgtgtattcc tgccattcgt atccctgctc aatccggtag aaattcccac gccaggaatc
     4381 gccacttttg ggcgggatat tcggagccgt aaccagttcc cggaatggaa tcgcacactc
     4441 catggtccag gacctgtcct cggaaagcgg atcggaggga tcaccatcca ctacaacggc
     4501 gtgtttcaca ccttctgcgt tccagtcacg aagcggtttg aagtggcccc atcggttcag
     4561 cacgaagacg tcagtcaaag tgttcaaagg
//
LOCUS       DAOSOO010000236         4577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_18940_length_4577_cov_5.134675, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000236 DAOSOO010000000
VERSION     DAOSOO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18940_length_4577_cov_5.134675"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLT86_12890"
     CDS             <1..206
                     /locus_tag="PLT86_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37550.1"
                     /translation="AHNHQSFAERYLFFYRTLIDRGVQIVLGSDWHNLVDGEPTAFYP
                     AMLLGMKPRDMRFLEHWLGPCPD"
     gene            194..1117
                     /locus_tag="PLT86_12895"
     CDS             194..1117
                     /locus_tag="PLT86_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37551.1"
                     /translation="MSGLTLKGPVLRPTWNDLELHAVDLHIHAGTESPEPYSLLDLVS
                     YVVATGRRIVCLTDHAGRFLGASRKPLAHYPGSIEGWRRFAADVQEAREKYPDTIILF
                     GPETGFGGLTSDAFAPLFQTPGVDLFLGEPGAAPEGVSHGDYLIAGVEEIARCRDRYK
                     IPGFLVHPLRDVINRVCGKSGPGPAHPKHPQFPPLESFADPRAHTEEVLGVDIGRLAE
                     TCVRLDVPVEINESDFGRIIGQNHQSFLDRYFLFNKSLIDAGVPVVLGSDMHNVERPA
                     CTPFFPATILGVKPRDIHFLERWMGKIHASC"
     gene            complement(1194..>4577)
                     /locus_tag="PLT86_12900"
     CDS             complement(1194..>4577)
                     /locus_tag="PLT86_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT37552.1"
                     /translation="GVTDADGAFSILQVPPGTHEVQANMVGFRTLISRGVVVSADRTT
                     TLNLSLREEAIQVDALVVTAARPAIETDVTSSRTTVNASRAAEVPVNQILDALAYEPG
                     VSLSRDNELNVRGGGPTQIRFQVDGLDRTDGLTNKPYTQLNQTLVAEVTLLTGGFNPE
                     YGNVRSGVVNVVTKDGRERGPNRGWVAGVYSVTPTARKAHYGPSAYSEDQFDYRTVLM
                     SDSTTIGANGLPDPYGPIYWPNVYEQTRNDTAFRRVWTAAPSVYKIFDGWAARTALAN
                     ANNRGAGVYGKGTRTKPWTPQQLIEAWKYEANLNEQVWGYGDERSWSVDVAAGWGLPR
                     QLGGIVVGLVTNKEMTPTPALRPYYRDQTFDAKLTLTPLDRLRVSVSYMHGTGVSTGS
                     GYAPYDPEMASTAGYVATGSDAVSLRTAGDLIGTVVQSKTSDGNNRLNLSYSDLLDAV
                     FWQWGTSATYTVNPATFITAAFGQTHTSYNLRKDAPRADATDFSPTSSYKPPSTFGYR
                     AWLLMLSSDWSDVDGNGTSDPPTSLADALTPGRVYLANPFGLPAAYHEVPANPVFVTR
                     QIVFAPGDTARVVSPQGWLQDGTYDLSGTFWLGGGGQWWMNSRSVQTSARADLTRVMG
                     AHTVKTGVEYIGGDLEFHSEEDSRLFGGRHLCNYRDYGGERPAVRPVILGLFAQDKFE
                     SEGMVMNFGLRAERFDGGAPYYMPDMMFDSRLFGPNNGKYWFDSLCVASGWDVTNPAW
                     GPLPGTPVEAWMKYVANGVPDKFPMPSDVMAALPQRDAKVHWRLAPRFGISHPVSENT
                     KFFFNYGIFYSMAKPAQMYGVGTHMRRVGSTGRFEHMFNPELRPARTAAYEVGFEHVF
                     PQNFLFRATGYAKHNVDQVTGLQVITGTGDGYRTYRNANYEDIRGLEMKLTRTGRFVS
                     AWATYEIIATRTGQTGLDRLHQNIAMVTYYTPLVSTNSPAGSVTASVRVGTPLEWGLI
                     RGGWGATILQSYSESTGEILYNPNGVPRRELPPEYVLRGRDSWGTSAKFTKDALVGRN
                     RRISVYLDISNLFNRKYLNGTYMNGGEYLSYIVERRAQGETDLRVGDPSTWDALTKPY
                     RIKRADGTYTAWKAPISPRTDWLGFLSPRAYRAGIRFDL"
BASE COUNT          841 a         1500 c         1330 g          906 t
ORIGIN      
        1 tggcgcacaa ccaccagtcc tttgcggagc gatacctgtt cttctatcgc acgctaatcg
       61 accgcggcgt gcagattgtc ctgggcagcg actggcacaa cctggtggat ggggaaccca
      121 ccgccttcta tcccgccatg cttctcggaa tgaaaccgcg tgatatgcgc ttcctcgagc
      181 actggctcgg gccgtgtccg gattgactct gaaagggccc gttttgcgcc ccacatggaa
      241 cgacctcgaa ctccacgcag ttgacctgca catccacgcg ggcactgaaa gcccggaacc
      301 atacagcctc ctcgatctcg tctcgtacgt ggtagcaacc ggaaggcgga tcgtctgcct
      361 gaccgaccac gccgggcgct tcctgggcgc ttcccggaag ccgctcgcgc attaccccgg
      421 ctcgatcgaa ggatggcgca ggttcgccgc tgacgttcaa gaagcgcgtg agaagtaccc
      481 cgataccatc atcctgttcg gcccggaaac cggattcggc gggctgacat cggacgcgtt
      541 cgcgccgctg ttccaaacac ccggcgtaga cctgtttctc ggcgaaccgg gtgcggcacc
      601 ggaaggcgtg agccacggtg attatctgat cgcaggcgtc gaagaaattg cccgttgccg
      661 ggatcggtac aaaatccccg gctttctcgt gcatcccctc cgcgacgtga tcaaccgggt
      721 ctgcgggaaa tcgggtcccg gccccgcgca cccgaagcac ccgcagttcc cgccgctgga
      781 atcattcgcc gaccctcgcg cccacacgga ggaagtcctg ggcgtggaca tcggccggct
      841 cgcggaaacg tgcgtgcggc tcgatgtgcc ggtggagatc aacgagtcgg atttcggccg
      901 catcatcggc cagaatcacc agtcgttcct cgaccggtac ttcctcttca acaaatccct
      961 gatcgatgcc ggcgttcccg tggttctggg cagcgacatg cacaacgtag aacgcccggc
     1021 atgcacgcca ttcttccccg ccaccatcct cggcgtcaaa ccgcgggaca tccactttct
     1081 ggagcgctgg atgggaaaga tccacgcgtc gtgctgacaa cgaaaaaaag ggcgccctgg
     1141 tatccgatca ggagcccttt cttccttcgc tcagtgcttg ccgcctcaaa gccctacaga
     1201 tcgaaacgga tccccgcccg gtatgccctc ggcgaaagga agcccagcca atcggtgcgc
     1261 gggctgatcg gcgctttcca cgcggtgtag gtcccatccg ctctctttat gcggtacggc
     1321 ttggtcagcg catcccacgt ggagggatct cccacccgca ggtcggtctc gccctgcgcg
     1381 cgtcgttcca cgatgtacga caggtattcc ccaccattca tgtaggtgcc gttcaggtat
     1441 ttccgattga acaggttcga aatgtcgaga tacacggata ttcgcctgtt acggccgacc
     1501 agtgcgtctt tggtgaactt cgcgctggtg ccccaggaat cccgcccccg gaggacatac
     1561 tccggaggaa gctcgcgcct cggcacaccg ttcgggttgt acaggatctc gcccgtgctt
     1621 tccgaataac tctgcaggat ggtggcgccc cagcctcccc tgataagccc ccactcgagc
     1681 ggcgtaccta cccgcacaga cgccgtgacg gaacctgcgg gagagttggt ggaaacgagc
     1741 ggcgtgtaat acgtcaccat cgcaatgttc tggtggagcc tgtcgagccc ggtctggccc
     1801 gtccgcgtgg cgataatctc gtacgtggcc cacgcgctca cgaacctgcc ggtgcgtgtc
     1861 agcttcatct caagcccgcg gatatcctcg taattggcgt tcctgtacgt gcggtacccg
     1921 tccccggttc ccgtaatcac ctgcagcccg gtaacctggt cgacgttgtg cttcgcatac
     1981 cccgttgccc tgaaaaggaa attctgcggg aacacgtgtt cgaaccctac ctcgtatgcg
     2041 gcggtcctgg ccggtcgcag ctcggggttg aacatgtgct cgaatcgtcc agtcgaaccc
     2101 acgcgccgca tgtgtgttcc gacgccgtac atctgcgcgg gtttggccat cgaatagaag
     2161 atgccgtagt tgaagaagaa cttggtattc tcgcttaccg gatgcgaaat gccgaaacgg
     2221 ggagcgagac gccagtgcac cttcgcgtcg cgctgaggaa gtgccgccat cacgtccgat
     2281 ggcatcggga atttgtcggg cactccgttg gcaacgtact tcatccacgc ctcaaccggt
     2341 gtgcccggca acgggcccca cgcagggttc gtaacgtccc acccggaagc aacacacagg
     2401 ctgtcaaacc agtatttgcc gttgttaggc ccgaaaagac ggctgtcgaa catcatgtcc
     2461 ggcatgtaat acggcgcgcc accgtcaaac cgctccgcgc gcaggccgaa gttcatcacc
     2521 atcccctcgg attcgaactt gtcctgcgcg aacagaccga ggatcacagg tcgaaccgcc
     2581 gggcgctccc caccgtaatc gcggtagttg cacaggtggc gccctccgaa caggcgcgag
     2641 tcctcttccg agtggaactc gaggtcgccg ccgatatatt ccacacctgt tttgaccgta
     2701 tgcgcgccca tcacgcgggt aagatcggcg cgggctgaag tctgcaccga ccgggagttc
     2761 atccaccact ggccgccgcc gcccagccag aacgtcccgc taagatcata cgtgccgtcc
     2821 tgcagccacc cctgcggtga caccaccctg gccgtgtcgc ccggggcgaa cacaatctga
     2881 cgcgttacaa acacggggtt ggccggcact tcgtggtagg ccgccggaag tccgaacgga
     2941 ttggcgaggt atacccgacc cggtgtcaac gcgtccgcaa ggctggttgg cgggtcggat
     3001 gttccattcc catctacgtc tgaccaatct gatgacagca tcaggagcca ggcccggtac
     3061 ccgaacgtgc ttggaggttt gtagctggag gttggcgaga aatccgttgc gtcagcccgc
     3121 ggagcgtctt ttcgaaggtt gtaactggta tgcgtctgtc cgaatgcagc cgtgatgaat
     3181 gtcgccgggt tcaccgtgta ggtggcggac gttccccatt gccagaaaac ggcgtccaga
     3241 agatcgctgt aggaaagatt gagccggttg ttgccgtcgg acgtcttcga ttgcacgacc
     3301 gtccctatca aatcaccggc agtgcggagc gaaacggcgt ccgatcccgt cgcaacgtat
     3361 ccggcagtcg aagccatctc agggtcatac ggtgcgtacc cggacccggt ggaaaccccc
     3421 gtcccgtgca tgtaggatac gctcacccgg agcctgtcca gcggcgtaag agtaagcttc
     3481 gcatcgaacg tctgatcgcg gtagtacgga cgaagtgccg gcgtgggtgt catctctttg
     3541 ttcgtcacca gccccaccac aataccgccc agttgccggg ggagtcccca gcccgccgca
     3601 acgtcaacac tccagctgcg ctcgtcaccg tatccccaga cctgctcgtt cagatttgcc
     3661 tcgtacttcc acgcctcgat caattgctgc ggcgtccacg gttttgtccg agtgcctttc
     3721 ccgtacacgc cggcgccccg gttattcgca ttcgccagcg cggtccgcgc agcccagcca
     3781 tcgaaaatct tgtacacgga cggagcggcc gtccagacgc gtctgaatgc ggtatcgttg
     3841 cgtgtctgct cgtatacgtt gggccagtag atcgggccat acgggtccgg caacccgttg
     3901 gcgccaattg tcgtcgaatc cgacatcaac accgttcggt aatcgaactg gtcctcgctg
     3961 taggcgctgg gcccgtaatg ggctttgcgc gccgtcgggg tcacgctgta cactccggcg
     4021 acccacccgc ggttcgggcc ccgttccctg ccgtcttttg tcaccacatt gacgacaccc
     4081 gagcgcacat ttccgtattc cgggttgaag cctcccgtca gcagggtaac ttctgccacc
     4141 agcgtctggt tcagttgggt gtagggcttg ttggtaagcc catccgtccg atcgagtcca
     4201 tccacctgga atctgatctg cgtcggcccg cccccgcgca cgttcagctc gttgtcgcgg
     4261 gaaagtgaca caccgggttc gtacgccagt gcatcaagaa tctggttcac cggcacttcc
     4321 gcagcgcggc tcgcgttcac ggtggttcgg gaggatgtga cgtcggtctc aatcgccggg
     4381 cgcgccgccg ttaccaccag cgcgtccacc tgaatggcct cctcgcggag ggaaagattc
     4441 agggtggtcg tgcggtcggc cgataccacc acgccgcgcg aaatgagcgt gcggaacccc
     4501 accatgttcg cctggacttc gtgcgttcca gggggtacct gcaggatgga aaatgctccg
     4561 tctgcatccg tcacgcc
//
LOCUS       DAOSOO010000237         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_19178_length_4537_cov_4.709505, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000237 DAOSOO010000000
VERSION     DAOSOO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19178_length_4537_cov_4.709505"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="PLT86_12905"
     CDS             <1..1901
                     /locus_tag="PLT86_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37553.1"
                     /translation="WIAPFEPGTYTLKTRVQKADGTILGETSERVPFVPFAREKQVAA
                     AILQELSGLSGSAAPTASYLGNLRARQTLFGIQAEQCLARGTEEEKRLLAEEGKKEIG
                     RLRAELARRRYALRTAAQHTGQTTEIVAWDPGHPWVPFDPDTDAPQSEPTDTIRVTTD
                     RRAHEATSVSVANLTGETISVRVWLDPWRGGETAPPLSAVALRRRVFVATARGGYTPD
                     ALPELDPSGVVTIPAGETLRLWLDWSSGDTPAGVYSSTLSVRALTVAGDVLRIPLQWE
                     VSTVALPDSMPLAFRVWAYEQPAFFERDAVWADLRAHYVNVYDIPVPTVRYNADGEIA
                     AEDWAAVEDVLRRAPSGSFFLWHGGETIVQPAEGAPGSGSPAWEKAFQTFVPRWLDGL
                     AARGVLPDRHANYILDEPGIDGGPSVYELIRIARLYKTADPRVRIFANPAGGATAQHV
                     DDLNEVVDIFDPIMNFAHLERVLAKGKPTWTYECGDGAKDNTRMRYYWQPIWQGTEMG
                     LSGLGIWSYAGRSVDFWQGPHDGCCDWELVYHGAGGSVIPSHRWQGVRIGIEDYARLW
                     LLSRAADNARGRGDTARAMELTRSRAAMIRQVLESGCDEGVVTRVRSQLRAILAAEIG
                     QQRTERND"
     gene            1985..3217
                     /locus_tag="PLT86_12910"
     CDS             1985..3217
                     /locus_tag="PLT86_12910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37554.1"
                     /translation="MTGKVIDRVVFMPSPCKGVATHASSYYTTREGGNLTSIHGYESR
                     SDTTDVAFVRFSDDNGRTWSAEETFQTEFPDPKGTRRRYPAGGYVDPLTGRYLTVRNE
                     GVLPTDDPLEGMRQWKLHYSVSEDGGRTFLVDEQIIHDGEEYDAVNHLPGISVGKTCA
                     MIGDMGQRPITRADGVLLVPIQYTPAGPDGMYLNPGAGFTYTNCMVLFGHWLPGGRLR
                     WTASDKIIADPERTTRGLIEPTIAQLPDGRIIMVMRGSNDKRPDLPGWKWYSESRDGG
                     QTWSVPAPWTYDDGTTFHSPSSCSQLYRHRDERLFWIGNICEENPQGNSPRYPLVVGQ
                     VDEVTGRLLRRSVWAVDDRKPGESPFLTLSNFYAREDRETGHVLIHMARLFARDFREG
                     GSIDWTADSLQYRIELED"
     gene            complement(3274..4089)
                     /locus_tag="PLT86_12915"
     CDS             complement(3274..4089)
                     /locus_tag="PLT86_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT37555.1"
                     /translation="MNRGAVTSVSMLLAALAFLSLSCSRGPKAGDVIKVKGKEMVYIP
                     GGTVTLGDSTMGRLTVTLKPYLIDKHEVTNGEYREFAKVFGLPFSVAPAYDDLPVVNI
                     PWETADAFAAWRGCRLPTEAEWEFAARGTDGRRYPWGNSADAEKANGDDGVDIMTVPD
                     GSKDGFSGLAPVGMFPKYASPFGVEDMSGNVWEWVQDWYAPYPPNAVSDYAGPDSGEQ
                     KVVRGGSFKTNFLNLRTFHRAYRVPGAAADDVGFRCASDYPPREPGALPSTVR"
     gene            complement(4224..>4537)
                     /locus_tag="PLT86_12920"
     CDS             complement(4224..>4537)
                     /locus_tag="PLT86_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37556.1"
                     /translation="FCYREFGRSQQESSMVRGVAAMLSSRDVLDVLDHLRKGLRGLRD
                     AAEIDRDPSLQKAINALQSQLSGVISSVEAVARRSGIIPKETILPRPSHGENREESPS
                     T"
BASE COUNT          943 a         1344 c         1382 g          868 t
ORIGIN      
        1 gttggatcgc cccgttcgaa ccggggacat acacgttgaa aactcgggtc caaaaggccg
       61 atgggacaat cctgggtgaa acctcagagc gcgtcccatt cgtgcccttc gcccgagaga
      121 agcaggtcgc ggcagcgatc cttcaggagt tgtcagggct gtctgggagt gcggctccga
      181 cggcctcata tttgggcaat cttcgcgccc gacagacgct ttttggcata caggctgagc
      241 agtgccttgc gcgtgggact gaggaggaaa agaggcttct cgcggaggaa gggaagaaag
      301 agatcggcag gctgagagct gagctggccc gccgccggta cgctttgcgg acagcggccc
      361 aacacaccgg gcagacaacg gagatagtcg cgtgggatcc gggtcacccg tgggtgccgt
      421 ttgatcccga taccgacgct cctcaatccg aacccactga taccatacgg gtaacgacag
      481 accgacgcgc gcacgaagca acctcagtgt ccgtcgccaa ccttaccggc gagacgattt
      541 cggtgcgcgt atggttggat ccctggaggg ggggcgaaac agcccctcca ctcagcgcgg
      601 ttgctcttcg ccgcagggtg ttcgtcgcga cggcccgggg tgggtacacc cctgacgcgt
      661 tacccgagct cgatccttcc ggcgtggtga ccatccctgc aggcgaaacg ctccgattgt
      721 ggctcgactg gtcatcgggc gacacacccg cgggagtcta ttcctcaacg ctttccgtgc
      781 gtgcgctgac tgtcgcaggg gacgtactgc gtatcccgct ccaatgggaa gtgagcaccg
      841 tcgccctccc ggattccatg ccactggctt tccgcgtctg ggcatatgaa caacccgctt
      901 tctttgaaag ggacgcagtc tgggctgacc tccgggccca ttacgtgaat gtgtacgata
      961 tcccggtgcc caccgtgcgt tacaacgctg acggggaaat agccgcagag gactgggccg
     1021 ccgtggagga tgttcttcgg cgcgccccga gcggctcgtt cttcctgtgg cacggtggcg
     1081 aaaccatcgt ccaaccggcg gaaggtgccc cgggctcggg ttctcctgcg tgggaaaaag
     1141 cgttccagac cttcgtgcca cggtggttgg acggtctggc cgcgcgcggg gtgctgccag
     1201 atcgccacgc aaactacatc ctcgacgagc ccggtatcga cggcggcccc agcgtgtacg
     1261 aattgatacg tattgcccgc ctgtacaaaa cagccgatcc ccgggtgagg attttcgcca
     1321 acccggcggg aggtgcgacc gcgcagcatg ttgacgattt gaacgaagtc gttgacatct
     1381 tcgatcccat catgaacttc gctcatctcg aacgggttct cgccaaaggc aagcccacgt
     1441 ggacctatga gtgcggcgat ggcgcgaagg ataacactcg gatgcgctac tactggcagc
     1501 caatctggca aggcacagag atgggccttt ctggtctggg catctggtcg tatgccggga
     1561 ggagcgtcga tttctggcag ggcccccacg acggctgctg tgattgggag ctcgtgtacc
     1621 acggcgcggg cggttctgtg ataccgagtc atcgctggca gggagtgcgg attggaattg
     1681 aggactacgc gcgcctgtgg ttgctttcgc gggcggcgga caacgcgcgc gggcgaggtg
     1741 ataccgcacg cgccatggaa ctcacacgca gcagagcagc gatgatacga caggttctgg
     1801 aatccgggtg cgatgaaggg gttgtaacgc gcgtcagaag tcaacttcgc gcaattcttg
     1861 ccgcagagat cggccaacaa agaaccgaac gaaacgatta gtgctcgtcg gcaacaatct
     1921 gtgaagccat caaagcccgt aacgaagtgc tgcaggcgga caattgagca gggggttcat
     1981 gcgcatgacc ggaaaagtga tcgacagagt cgtcttcatg cccagtccgt gcaaaggagt
     2041 tgcaacgcac gcatcctcgt attacacgac tcgtgaaggt ggtaacctca cgagcattca
     2101 cgggtacgaa agtcgaagtg acaccacgga cgtggcgttt gttcgattct cggacgacaa
     2161 cggtagaacg tggagcgctg aagaaacgtt tcagaccgaa ttccccgacc cgaaaggaac
     2221 gcgacgcagg tatcctgcgg gagggtacgt ggatccgctc acgggaaggt acctgaccgt
     2281 gcgtaacgag ggagtgctgc cgacggatga tccccttgaa gggatgcgtc agtggaaact
     2341 ccactactcg gtttcagaag atggtggcag gactttcctt gttgacgagc agattatcca
     2401 tgatggagaa gagtacgacg cagtcaacca cctcccgggg atttcggtcg ggaagacatg
     2461 tgcgatgatc ggagatatgg gacagagacc gatcacacgt gcggatgggg ttctcctcgt
     2521 tcccatccag tacacccccg ccgggccgga tggcatgtat ctgaatcccg gcgcggggtt
     2581 cacctacacg aactgcatgg tcttgttcgg gcattggctt ccgggagggc gcctccggtg
     2641 gacggcgtct gacaagataa ttgcggatcc ggaacgaaca accaggggac tcatcgaacc
     2701 aacgatcgcg cagcttcccg acggccgcat tatcatggtg atgaggggca gcaacgacaa
     2761 gcgtccggac ttgcctgggt ggaaatggta ctcggaatcc cgtgatggcg ggcaaacgtg
     2821 gagtgttccc gctccgtgga catatgacga tgggaccacg ttccattcac cgagttcctg
     2881 ttcgcagcta taccgacacc gcgacgaaag gttgttctgg atcgggaaca tctgcgagga
     2941 aaacccgcag ggaaacagcc cgcggtaccc actcgtcgtt ggtcaggtgg acgaagtgac
     3001 gggacgcttg ctacggagaa gcgtgtgggc agttgacgac cgcaagcctg gcgaaagtcc
     3061 cttccttacg ctgtccaact tctatgcgcg ggaggacagg gagacgggtc atgtgctgat
     3121 acacatggcg cgcctcttcg ccagggattt ccgggaggga ggctccatcg actggaccgc
     3181 cgacagcctt cagtatcgaa tcgaactcga agactgagtg acgcggcgta acgacggcgg
     3241 ggcagaaccg actgccccgc caaatgctgc aattcacctt acggtggaag gcaacgcgcc
     3301 cggttcacgc ggagggtagt cgcttgcgca ccggaacccc acatcatccg ccgcggctcc
     3361 gggaacgcgg tatgcccgat ggaacgtgcg gagattgagg aaattcgtct tgaagctccc
     3421 cccgcgcacg accttttgct cgccgctgtc ggggccagcg tagtcagaaa ccgcatttgg
     3481 cggatatggc gcataccaat cctgaaccca ttcccagacg ttcccgctca tatcctcgac
     3541 gccaaacggg cttgcgtatt tcgggaacat tcccaccggt gcaagacctg aaaacccgtc
     3601 cttggaacca tcgggaacag tcatgatgtc gacgccgtcg tcgccgttgg ccttttccgc
     3661 gtcagccgag ttgccccagg ggtaccgcct tccgtcagtt ccccgcgccg cgaactccca
     3721 ttcggcttcg gtcggcaggc gacatcccct ccacgccgca aacgcatcgg cggtctccca
     3781 cggtatgttc acaacaggga ggtcgtcgta cgccggagcg acagagaacg gtaagccgaa
     3841 taccttcgcg aattcgcggt actccccgtt cgttacctcg tgcttgtcga taagataggg
     3901 cttcagcgta accgtcaacc ggcccattgt cgaatcgccg agcgtcacgg ttccgcccgg
     3961 gatgtacacc atttccttgc ccttgacctt gatcacatct cccgctttgg ggccgcggct
     4021 gcacgaaagg gacaagaatg ccaacgctgc aagaagcatt gagaccgacg taacggcgcc
     4081 acgattcatg ttcgctctcc ctattccacc ccggcggagg gggcgtcacc tgtcagaatc
     4141 acgaaaacga gggcaaaagc gtcgctctcg ccctcgtttg tagtatcgca agaacaccgt
     4201 caggatcaaa cgggacgcgg tattcaggtg gacggtgact cctcacgatt ctcgccgtgg
     4261 cttggcctcg gcaggatcgt ttccttggga atgattccgc ttcgcctcgc caccgcctcc
     4321 actgaggaga tcacgcccga caactgactt tgaagtgcat tgatagcctt ctgcagagac
     4381 ggatcccggt cgatctccgc ggcatcgcgc aatccgcgca gccctttcct caggtggtcc
     4441 agaacgtcga gaacatcccg tgatgacaac atggccgcca cgccccttac cattgaactt
     4501 tcctgttgcg atctcccgaa ttctctgtaa caaaatg
//
LOCUS       DAOSOO010000238         4525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_19237_length_4525_cov_5.149664, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000238 DAOSOO010000000
VERSION     DAOSOO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19237_length_4525_cov_5.149664"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLT86_12925"
     CDS             complement(<1..389)
                     /locus_tag="PLT86_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT37557.1"
                     /translation="MMRLPTPGSPGAYLLAARPKTLPAAIAPVLVGSACAFYEGMFAW
                     YPASLALVCAILIQVGANFANDLLDFEKGHDTTTRVGPVRAVQTGLLTREQMRAATVA
                     VFGSACVLGLLLTLHSGWPVAAIGIAS"
     gene            complement(386..1225)
                     /gene="menB"
                     /locus_tag="PLT86_12930"
     CDS             complement(386..1225)
                     /gene="menB"
                     /locus_tag="PLT86_12930"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOT37558.1"
                     /translation="MEHTQDVPAWDPVPGCDFSDILYHKAEGIAKITINRPKVRNAFR
                     PLTVQEMIAAFDDARDDETIGAVILTGAGNEAFCSGGDQKTRGAGGYVGADGIPRLNV
                     LDLQRRIRTIPKPVIAMVAGYAIGGGNVLQLVCDLTIAADNARFGQTGPKVGSFDGGY
                     GSSYLARVVGQKKAREIWFLCRQYDAREALEMGLVNKVVPLAELERETVQWCREILQH
                     SPTALRFLKSALNADCDGQAGLQQLAGDATLLFYLSEEAKEGRDAFLQKRRPDFRRFT
                     RLP"
     assembly_gap    1328..1427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1428..3153)
                     /gene="menD"
                     /locus_tag="PLT86_12935"
     CDS             complement(<1428..3153)
                     /gene="menD"
                     /locus_tag="PLT86_12935"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOT37559.1"
                     /translation="MTFGDIQFLWARGIVETLLQAGVRCFVVSPGSRSTPLVLALATT
                     RDIVVHKILDERSAGFFAVGYAKATGQTVCLVCTSGSAPAHYLPAIVEAAHSFTPLLV
                     LSADRPPELHGNAAPQSVNQAHLFPSFSRLFVDLGIPESGDAALRAVTRKVTQAAVAA
                     RLPEPGPVHINVPFREPLEPSAIGITSPRPAVAAAPQFVAPRSALLDTGTVKSIAQLV
                     RSKPRGLIVAGPAPIGQSACRDVIGDLAWRTGFPLLAETASQLRFTGTRMDHRVDAFD
                     LILRSPRARARLQPELVIQLGAPPVSKSLARMLDEAPEIRRIVIAPFGWNDPQNTASI
                     HVRADVETVCRQISAELTVPAGTSSWAEEWSRVGEAVWQFLERGFAEAETRLSEATVA
                     RFTAASLREDDILVVSNSLPIRDLDWFCHGESSCFRVVSQRGAAGIDGSVSMALGVAS
                     SHTSRTTLLVGDSAFLHDVGALNIAAAARVPLRIVVANNNGGRIFQKLPIARREEFRE
                     LLDPWFVMPHGLDISNVAASFGVHATRVETARDLLKALTDPAPDDKPFVVEALIDPAV
                     SKKHYGLLE"
     gene            complement(3140..>4525)
                     /locus_tag="PLT86_12940"
     CDS             complement(3140..>4525)
                     /locus_tag="PLT86_12940"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOT37560.1"
                     /translation="VSDEAFSFLREQLSAPIGRNCFRAITVPIERTSLTGALKFDGTE
                     TWIYWATPSGEETLAWGAAWKRALATQNRFAAAERSVSTLWDRIERRSFPGTRKPNLK
                     VFSGFAFDPAGPHDGEWDGIPSNYCVLPSICITCSRGSCRATVISGRDSYREALHGFS
                     DLLGSLSPPLTGTQENRAFTLDAAHEATGENQIAQWVRSIASIKDGITSGRFQKVVAA
                     RSVLHSLSRTPDIRKALDLLALRYPDSYRFAAFLGKKLFLAATPELLVHKRASRFTTH
                     ALAGSMPFGANAQAKYAARRALLRSAKDSDEHSLVVEAIRRSVGGFAKTLEVSKSPRI
                     ILYGDIAHLETPLEGVLEHDVHVLRLVSAIHPTPAVGGVPSDEALEWIRANEEVPRGW
                     YAGPIGWFDENGDGCFVTAIRSALLAPGSARLFAGAGIVADSDPVTEYEETEAKLKPM
                     RLALGLSNDVW"
BASE COUNT          912 a         1334 c         1311 g          868 t
ORIGIN      
        1 atcgaggcaa tgcctatcgc cgccaccggc cagcctgaat ggagtgtgag cagcaatccc
       61 agaacgcacg cggacccgaa cacggcaacc gtcgccgcgc gcatctgctc tctggtaaga
      121 agaccggttt gaacggcccg taccggaccg acgcgcgtag ttgtgtcgtg ccctttctcg
      181 aaatcgagaa ggtcgttggc gaaattggct ccaacctgga taagaatcgc gcacacgagt
      241 gcgagggatg ccggatacca cgcgaacata ccctcgtaga acgcgcacgc gcttcccacc
      301 aacaccgggg cgatagcggc aggaagggtt ttgggccgcg cagccagcag gtatgcgccc
      361 ggggaaccgg gtgttggcaa ccgcatcatg ggaggcgtgt gaacctccgg aaatcggggc
      421 gacgcttctg caggaacgca tcacgcccct ctttcgcttc ttcagacaga tagaagagca
      481 gtgtcgcatc gcccgcgagt tgctgcagac ccgcctgtcc gtcgcaatca gcattgagag
      541 ccgatttgag gaaccggagc gcggtcggac tgtgctggag aatctcgcgg caccactgaa
      601 ctgtctcgcg ttcgagctcc gcgagaggta cgaccttgtt tacgaggccc atttcaagag
      661 cctcgcgtgc atcatactgc cggcagagaa accagatttc gcgtgctttc ttctgcccca
      721 ccacacgcgc cagatacgag gacccgtacc ccccgtcgaa actgccgact ttgggaccgg
      781 tttgcccgaa tcgcgcgttg tccgcagcaa ttgtcagatc gcagaccagc tgaagaacat
      841 tcccgccacc tattgcatag ccggctacca tggctatcac cggtttggga atggtgcgta
      901 tccggcgctg gaggtcgagc acgttcaggc ggggtatccc gtccgcgccc acgtaacctc
      961 ctgcccctct cgttttctgg tctccgcccg agcagaacgc ttcgtttcct gcccccgtaa
     1021 ggatgacggc accgatggtt tcatcgtcgc gcgcgtcgtc gaacgccgcg atcatttcct
     1081 gcacggtcaa cggccggaac gcattccgca cctttgggcg attgatggtg attttcgcaa
     1141 tgccctcagc cttgtggtac aggatatccg agaaatcgca tccgggaacg gggtcccatg
     1201 cgggaacatc ttgggtgtgt tccatggttc agtccttgtc gcgcagctgg tcagcggcca
     1261 gtttcgatgc gttttcgagt agtccgtaat gctttttcga aactgccggg tctatcaaag
     1321 cctcaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt cgagtagtcc
     1441 gtaatgcttt ttcgaaactg ccgggtctat caaagcctca acgacgaagg gtttgtcgtc
     1501 tggggctgga tcggttagag ctttcagcag gtcgcgcgcc gtttcaacgc gtgtggcatg
     1561 cacgccgaaa gaagctgcga cgttcgaaat gtccaatccg tgaggcatca cgaaccaggg
     1621 atccagcagc tcgcggaatt cttcacgacg cgcgatgggg agtttctgaa atatgcggcc
     1681 cccgttgttg ttcgccacaa caatacgcag cgggacgcgc gccgccgcag cgatgttcaa
     1741 cgcgccgacg tcgtgcagga aggcgctgtc tccgacgaga agcgttgttc gtgatgtgtg
     1801 tgaagacgcg acaccgagag ccatcgaaac ggacccgtct atgccggcgg cacccctctg
     1861 actcaccaca cggaaacagg acgactcgcc gtggcagaac cagtcgagat cgcgtatggg
     1921 caagctgtta ctgacgacga ggatatcatc ctcgcgaaga ctcgccgcgg tgaaccgggc
     1981 aaccgtcgcc tctgaaaggc gcgtttcggc ttctgcgaat cctctttcca gaaactgcca
     2041 cactgcttca ccaacgcgcg accactcttc ggcccatgac gacgtaccag cgggcaccgt
     2101 taactccgcg gagatctgcc ggcacaccgt ttcgacgtcg gcccgaacat ggatcgaagc
     2161 cgtgttctgc gggtcattcc agccaaacgg ggcgatcaca atccgccgga tttccggcgc
     2221 ttcgtccagc atgcgcgcca aagatttgga gacgggtgga gcaccgagtt gaatgacaag
     2281 ctccggttga aggcgtgcgc gtgcgcgagg ggagcgcaga atgaggtcga acgcgtcaac
     2341 gcgatgatcc atacgcgttc cggtgaagcg caactggctg gctgtttcgg ccagaagcgg
     2401 aaagcccgtg cgccaagcca ggtccccgat tacatcccgg cacgcgcttt gcccgattgg
     2461 agcgggaccg gcgactatga gtccccgcgg tttgcttcgg acgagttgcg caatggattt
     2521 caccgttccg gtgtcgagaa gggcggagcg gggcgcgaca aactgcggag ctgccgcaac
     2581 tgccggtctg gggctcgtga ttcctatcgc cgaaggttcc agcggctccc gaaacggaac
     2641 gttgatatgg acggggcccg gttccggcag acgcgcggca accgccgcct gggtaacctt
     2701 cctcgttacc gcacggagcg ccgcgtcgcc cgattctgga atccccagat cgacaaaaag
     2761 gcgcgagaac gagggaaaga gatgcgcctg gttgactgac tgcggcgccg cgttgccgtg
     2821 aagttcaggt ggccggtcgg cgctcagaac cagcagcggc gtgaaggaat gcgcagcttc
     2881 cacgatcgct ggcagatagt gtgcgggggc gcttcccgac gtgcatacaa gacagaccgt
     2941 ctgcccggtg gccttcgcat acccgaccgc gaagaatccc gcagagcgct catcgagtat
     3001 tttgtgaaca acgatgtcgc gcgttgtggc aagggcgaga acaagcggag tggatcgcga
     3061 ccccggactc accacgaagc accgtacgcc tgcctgaaga agggtttcca caatgccgcg
     3121 tgcccacagg aactgaatgt caccaaacgt cattgctgag gcccagggca agtctcatcg
     3181 gtttcaattt tgcctcggtc tcttcgtatt ccgtaaccgg atcggaatcc gcaacgattc
     3241 cagcgccagc gaacaggcgg gctgaaccgg gcgccaaaag ggccgagcgg attgcagtga
     3301 cgaaacagcc gtcaccgttt tcgtcaaacc acccgattgg tcccgcgtac catccccggg
     3361 gcacttcctc atttgcgcgt atccattcca atgcctcgtc ggagggaacg cctcccacgg
     3421 cgggagtcgg gtggatggca ctcacgagac ggaggacatg aacatcgtgc tcaagcactc
     3481 cttccaaggg tgtctccaaa tgtgcaatat caccgtacag gatgattcgc ggggacttcg
     3541 agacttcaag cgttttcgca aatcctccca cgcttcgccg gattgcttct actacgagag
     3601 agtgctcatc tgagtctttg gcgctgcgca atagagcgcg acgggccgcg tatttcgcct
     3661 gagcgttcgc gccgaacggc atcgaacccg caagcgcatg agttgtgaac cgtgacgctc
     3721 tcttgtggac gagcagttct ggtgttgccg ccaggaacag cttcttcccg agaaaagctg
     3781 cgaaacgata ggaatctggg taacgcagtg cgagcaggtc aagcgctttc ctgatgtccg
     3841 gcgttctgga caacgaatgc agaacgcttc tggcagctac caccttctgg aatcttcccg
     3901 acgttatccc gtctttgatc gaagcgatgg atcgaaccca ttgtgcgatc tgattctcgc
     3961 cggtggcttc gtgtgcggcg tccagcgtga atgcccgatt ctcttgtgtt ccggtgagcg
     4021 gaggggaaag gctccccaac aagtccgaaa aaccatggag cgcttcccgg tacgaatccc
     4081 gcccgctaat caccgtggcc ctgcacgagc cgcgagaaca ggtaatgcag atactgggaa
     4141 gcacgcagta gttcgaaggg ataccatccc attctccgtc atgaggcccg gccggatcga
     4201 atgcaaaacc cgaaaatacc tttaggttgg gtttccgcgt ccccggaaaa ctgcgccgtt
     4261 cgatcctgtc ccaaagggtg gagacagatc tttccgcggc agcaaacctg ttttgagtgg
     4321 ccagggcccg tttccatgcg gcgccccacg cgagggtttc ctcacccgac ggcgtcgccc
     4381 agtaaatcca tgtctctgtc ccgtcaaatt tcagggcgcc ggtgagcgat gttctctcaa
     4441 tcggaacggt gattgctcgg aagcagtttc ttccgatagg tgcgctcagt tgttcacgca
     4501 gaaacgagaa cgcctcatcc gagac
//
LOCUS       DAOSOO010000239         4519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_19278_length_4519_cov_4.835349, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000239 DAOSOO010000000
VERSION     DAOSOO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19278_length_4519_cov_4.835349"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLT86_12945"
     CDS             <1..788
                     /locus_tag="PLT86_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37561.1"
                     /translation="TGELRPHSATHGMLSMGRYYFAELRSNSCAGIGRWDLETGQFTE
                     VIRCDNTGHLKANPAGPEISFNVYEYREGTNEKGTRNFVKTEEYRHAESLERIEVTFP
                     KGRHGIAHSYWLGGTGRYQGTLQWPERGIAIMERDAPEPEIIPSPGPYFWHSGSSYDG
                     DWIIADTNFPYEGIWLVNVTTGKKELLCRPPLPDGADKVDKPLAHPHPNLSHDGSRAV
                     FTFSDDRSIPQVFVVEIPGDVRRRLGTRNGATREGGMESQVGF"
     gene            822..1805
                     /locus_tag="PLT86_12950"
     CDS             822..1805
                     /locus_tag="PLT86_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT37562.1"
                     /translation="MSKMDDLQLFDDLQPKLEQVRTELANVIVGQKTVIDHLLIGLLS
                     GGHCLIVGVPGLAKTLMVRSFAQALDLAFNRVQFTPDLMPSDITGSEILDETAGSGQR
                     SFRFVRGPVFTNVLLADEINRAPPRTQSALLEAMQEHHVTSSGQTHKLAEPFYVLATQ
                     NPIEQEGTYPLPEAQLDRFMFMLWVDYPSADEEVQVVRSTTGEDSGKVSTVLQRDEII
                     EAQALVRRVPVDGNVVLRAVNLVRRTRPIEGSPDFVRDFVSWGAGPRASQYLILAAKS
                     KALLEGRKTPAPEDVDALALPVLRHRIVTNFNAEAAGVKTPDIIARLLESA"
     gene            1802..2767
                     /locus_tag="PLT86_12955"
     CDS             1802..2767
                     /locus_tag="PLT86_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT37563.1"
                     /translation="MNVESNRAATQYRRFLDPQTLARLKGLDLVARFAVEGFMAGLHR
                     SPFHGFSAEFSDHRQYISGDPIRHLDWKVYAKTQRLYLKRYEEDTNLQAYLLLDRSGT
                     MAYGSDGLSKWDYARMVAASLAHLLLSQRDAVGLALFDYDVRRWLPARSTPGHRDALL
                     AEMDRFPAENPTDPSSALHVLAERVRRRSLIILISDMLIPAGAVAPSSGVTLADAMVE
                     RWVNSLKHFRHQGHDVLVFHVLHRDEVDFEFSGSVEFAGLEGEPGLLVDTWSVAHIYR
                     QQMSTYLRAVEKACGEIHVDYHRLFTSSPLDLALSSCLDKRRRLG"
     gene            2861..4396
                     /locus_tag="PLT86_12960"
     CDS             2861..4396
                     /locus_tag="PLT86_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT37564.1"
                     /translation="MSKRVLIAVMTGVVCMLARPPGSQAAVLTVLPPAHRFELDSTTG
                     VHYTVLADSGKSANLYFHQRSWLSDNSVVLYTGPTSAQRLMGYIQATGERIVIGNITG
                     ATAAARRNSVFGMRDRKLVEIALLITISNDPRSVPSRVEATERTIRTLTFTSQVNANY
                     DDTFLSVYENNESITPKRYSVYRISVSNGEMIEVCRASSSISHLQWSRGPSNLLMYSG
                     NDDARLWVVNPAEGVPRNVYVQVPSMGEWVTHESWWVNDQILWCGAPWPAGYELDPQR
                     GEYHHVNVLDLHSGISRIVAAGSWLPAQENSERWKRNFHHACGDEDGRWIVADSFSGV
                     LTLTEGKTTRTRLFTVGHRTYGGGEHSHPSFDRLGKQVVFVTNRFGEPRVCVARIPDD
                     WRAESAPIITSVEPAAEPPQRPGDLPFSIPALFPSEPNPFNPATLIRFSVPRTERVVL
                     NIYAINGQLVCTLVDATRQQGEHSARWEGRDSSGRLVGAGVYLCRLVTPSGTATERIV
                     VVR"
BASE COUNT          931 a         1284 c         1356 g          948 t
ORIGIN      
        1 ggaccggaga gctccgtccc cactccgcaa cgcatggaat gctctcgatg gggcgttatt
       61 acttcgcgga actaaggagc aattcgtgcg ccggcatcgg gagatgggac ctggaaacgg
      121 ggcaattcac cgaagtgatc cggtgcgaca acaccggcca cctgaaagcg aacccggccg
      181 gaccggaaat cagtttcaat gtgtatgagt accgagaggg aacaaacgaa aagggcacac
      241 gcaacttcgt gaaaaccgag gaataccgcc acgctgaatc actggagaga atcgaagtaa
      301 cgtttcccaa agggcggcac ggaattgcgc attcgtactg gctcggagga acgggcagat
      361 atcagggaac actgcaatgg ccggagcgcg gcatcgcgat aatggaacgg gatgcgcctg
      421 aacctgagat cattccttcc ccgggcccct atttctggca ttcgggctca tcgtatgacg
      481 gggactggat catcgcggat acgaacttcc cgtacgaagg gatctggctt gtcaacgtga
      541 ccacgggcaa aaaagagctg ctttgccggc ctcctctgcc agatggcgcg gacaaggtgg
      601 acaagccact cgcccaccct caccccaatc tttcgcacga tggttcgcgt gccgtgttca
      661 cattttccga tgaccggtcc ataccgcagg tgtttgttgt ggaaatcccg ggagacgtgc
      721 gcagacgcct cggcaccagg aatggtgcga cgagagaagg tggaatggaa tcccaggtcg
      781 gtttctgacg tggggaacga cagtgtcaga aaaaggaagt gatgtccaag atggatgatc
      841 tccagttgtt tgacgatctt cagccgaagc tcgaacaggt gagaacggaa ctcgcgaacg
      901 tcatcgtcgg gcagaaaaca gtgattgacc acctcctcat tggtctgctt tctggcgggc
      961 attgcctgat tgttggcgtg ccgggtctcg caaagactct gatggttcgt tcatttgcac
     1021 aggctcttga ccttgcgttc aatcgcgtgc aattcactcc ggatctgatg ccctcggaca
     1081 tcactggcag cgaaatcttg gacgaaactg ctggatcagg ccagaggagt ttccggttcg
     1141 tgcgcggtcc ggtgttcaca aacgtgctcc tcgcagacga gatcaatcgc gctccgccgc
     1201 gaacgcagtc agcgcttctc gaagccatgc aggaacatca tgtgacaagt agcgggcaga
     1261 cgcacaagct cgcagagcca ttctacgtcc tggcgacaca gaatcccatt gagcaggagg
     1321 gaacctatcc tcttccagag gcgcaattgg accgtttcat gttcatgttg tgggtggatt
     1381 acccttccgc cgacgaggaa gtccaggttg tccgctccac aaccggtgag gattctggaa
     1441 aggtatccac ggtactccag cgtgacgaaa tcatcgaagc tcaggctctc gttcgcagag
     1501 tccccgtgga tgggaacgtg gtcctgcggg cggtgaacct cgtgcgacgc acccgtccga
     1561 tcgaaggcag tcccgatttc gtgagggatt tcgtgtcgtg gggggccgga ccgcgggcaa
     1621 gtcagtacct cattctggcc gccaagtcga aagccttgtt ggaagggcgc aagactcccg
     1681 ctccggagga tgtcgacgcg ctggcgcttc ccgttttgcg acaccgcatc gtcacgaact
     1741 tcaacgcgga ggccgcgggc gtgaaaacac cggacatcat tgcgcgtctg ttggaatccg
     1801 catgaacgtc gagagcaatc gcgctgcaac tcagtatcgg agattccttg atccgcagac
     1861 gttggcgcgt ctgaagggtc tcgatctggt cgcccggttc gccgttgaag ggttcatggc
     1921 ggggctccac agaagtcctt ttcacggctt ttcggccgag ttctccgacc atcgccagta
     1981 catttccggt gatcccatcc gacaccttga ctggaaggta tacgcaaaaa cccagcggct
     2041 ctacctgaaa cgatacgaag aggacacgaa tctccaggcc tacctgcttc tcgaccgttc
     2101 gggtactatg gcgtatggaa gcgacggcct cagcaagtgg gactatgccc gcatggtggc
     2161 ggcctcgttg gctcatctgc tgctttccca gagagacgct gtcggcttgg cgttgttcga
     2221 ctacgacgtg cgacgttggc tgcccgcccg ttccacgccc ggtcatcgtg atgccctcct
     2281 tgcagaaatg gaccgcttcc ccgcagagaa tcctaccgat ccctcgtcgg cgcttcatgt
     2341 tctggcggaa cgggtgcgca ggagaagcct tatcatactt atcagtgaca tgctgattcc
     2401 cgccggtgcg gttgcccctt cctcaggcgt cacgcttgcg gacgccatgg ttgagcggtg
     2461 ggtgaactcg ttgaaacatt tccggcacca gggacatgat gtgctggtgt tccatgtact
     2521 gcatcgcgac gaggtggatt tcgaattctc aggttcggtg gagtttgcgg gtctcgaagg
     2581 agaacccggt ttgttggtgg acacgtggtc ggttgcccac atctaccggc aacagatgag
     2641 cacgtatctg cgtgcggtag aaaaagcgtg cggggagatt cacgtggact accaccgtct
     2701 tttcacttcc tctccacttg atctcgcgct gtcttcatgc ctggacaaac ggcggcggtt
     2761 ggggtagcga cgtggccggg gtgcaggttc actgcgttcg ggccggtttg ttccagagtg
     2821 gcgttcagat tccggttctt cgcagcaggg acgacgggac gtgagcaaac gagttctcat
     2881 cgcggtgatg acgggcgtcg tctgcatgct ggcgcgtccc cccggatcgc aggcggcagt
     2941 cctcaccgtt ctgcctcctg cccatcgctt cgagttggat tccaccacgg gtgtgcacta
     3001 cacagtgctg gctgactcgg gcaaatccgc gaatctgtac ttccaccagc gctcgtggct
     3061 ttcggataat tcggttgtcc tctacaccgg gccaaccagt gcccagcgat tgatggggta
     3121 cattcaggct accggcgaac gaatcgtcat cggcaatatc accggcgcaa ccgcggcggc
     3181 gagacgcaac tcggtgttcg gcatgcgcga caggaagctt gtcgaaatcg cgctgttgat
     3241 caccatatcg aacgatccac gaagcgtgcc ttcacgggtc gaggcgacgg aacgcacgat
     3301 acgaacgctc acgttcacgt cgcaggtcaa cgccaactac gatgacacgt ttctttccgt
     3361 ctacgaaaac aacgaaagca taacgcccaa acgctactcg gtgtacagga tttccgtttc
     3421 aaacggtgaa atgatcgagg tctgcagagc gagctcgagc atttcccacc tccagtggag
     3481 ccgtggtcct tcgaacctcc tcatgtactc agggaatgac gatgcaaggc tctgggtggt
     3541 gaatcctgcg gaaggcgttc ctcgcaacgt ctacgtccag gtgccctcaa tgggcgaatg
     3601 ggttacgcat gagtcatggt gggtcaatga ccagatcctg tggtgcggcg ctccgtggcc
     3661 cgccgggtac gaactcgatc cgcaacgagg cgagtaccac cacgtgaacg tattggacct
     3721 tcactcgggg atttcgcgga tcgttgcggc gggctcgtgg ctgccggctc aggaaaactc
     3781 ggaacggtgg aaacgcaact tccaccacgc ctgcggcgac gaagacggac gatggattgt
     3841 cgcggacagt ttcagcgggg tgctgacgct taccgaggga aaaaccactc gaacgcgact
     3901 tttcaccgtc gggcaccgca cgtatggcgg cggcgaacac tcccacccga gtttcgaccg
     3961 gctcggaaag caggtcgtgt tcgtcacgaa tcgatttggt gaaccgcggg tatgcgtggc
     4021 tcgcatccct gacgattgga gggcggaatc cgccccgatt atcacgtccg tggaacccgc
     4081 ggctgagcca ccgcaacgac caggcgatct ccccttttcc attccggcct tgtttccgag
     4141 tgaaccaaac ccgttcaacc cggccacgct gatacggttc tcggttccgc ggacggagag
     4201 agtagttctg aacatctatg cgattaacgg ccagcttgtc tgcacgcttg tggacgccac
     4261 caggcaacag ggcgagcatt ccgcccgttg ggagggccgc gattccagtg gtcgccttgt
     4321 gggcgctgga gtatacctgt gtcggctcgt aacaccgagt gggaccgcca cggaacggat
     4381 tgtagtcgtg cggtgaacgg ctttcgtgtg taaggatatg gacagaatga acaaaaccgc
     4441 gaagcttctc atgtttttgc tcgtgttttc cgtgtctttt ctggcgcgcg cagcgtgtgc
     4501 gacggacagc acgtacgtg
//
LOCUS       DAOSOO010000240         4444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_19689_length_4444_cov_4.598542, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000240 DAOSOO010000000
VERSION     DAOSOO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19689_length_4444_cov_4.598542"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PLT86_12965"
     CDS             complement(<1..1611)
                     /locus_tag="PLT86_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT37565.1"
                     /translation="MVVNRDYTRWLGNLKWMPRDSWIRNMHDPAAECAGNPVTLAGDS
                     GTIWISSPVPIPLDHANLQVSVAGTVRGGRIGIEWCHAKRGKFYPPVWAATEGDGRFL
                     LSQAGARRTTASFRFVVELGNERLAALEITECRGESQPELQVLVNQVGYNVGLPKTFT
                     VQCNAPLEVGGSFRVIAPIAGPGKEFVTVFEGALEPAGRIGEWERTYWRGDFSRLDIP
                     NNYYIEARVGDLEAESPVFALGNNFVAERTLDTAQRFYWYQRCGMAIPGWHEACHLDD
                     RITTADGYTRDVAGGWHDAGDYNKYNGFTPHSVWALLYTHEQRADLCRTIRNGPHCDI
                     LDEALWGAEFLRKMQEPVSGKLWDRVFSGYGHWNTPESETDNRFGDEKDRVAAGEARN
                     LWLVASFAHIGRVLRNAEFTDRALRHWKLCRDLGNEGLHHNAAQLIAAHELLRTTGDT
                     GFVEVEEQCVRHILACQGADENYDGWFANAPGGKPAYSIVDNGLPAASLALWVRANPT
                     RNLAVDVLGSLVRYTGFLTRIADNPFGIS"
     gene            complement(1643..2599)
                     /locus_tag="PLT86_12970"
     CDS             complement(1643..2599)
                     /locus_tag="PLT86_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT37566.1"
                     /translation="MRAVRMVAPRKMEIVEIEVPEISQGQVLVRLLRNAICGSDLITF
                     LETQPRRYPGELGSPCHECAGVVEKSFLDGFEPGDRVLYFPPPVENGLREYVAADAHQ
                     LLKLPAEGNLSELVLAQLLGTVLRTTREIGSVLGQSVAVIGQGPVGQLFDRILWNLGA
                     KQVIGIDSVPERLRISPLMGATAVVDATARQPWETVSRLTGGKGADLVIEAAGYEETQ
                     RLMIELVRQDGRIVMFGVPKYQRSELDLFAWYKKRAQMVTYHVPDVERDIALALQFIV
                     EKRVDVKPILTHAFPLTRVQEAYDLFADRREGCVKVILEMDR"
     gene            complement(2629..3714)
                     /locus_tag="PLT86_12975"
     CDS             complement(2629..3714)
                     /locus_tag="PLT86_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37567.1"
                     /translation="MKRYFHTLTSAFTSRMRGIDMVFRCGIVGIGSWYSQAFARAVQA
                     TSRHAKLIAAAHLGQSSESVKRYTGYTREGFADEFALNLYESARDMIHHENLDVVFVC
                     AHDAEKRKYAELALREGCHVYVAKPLCLSTVDAQALREIGRETGRTISTLEPGRYDGA
                     IREARTRVVNGEIGEVISARCWIQHGQPGKMDLSESVEMSPRSGGTLFTLGVYAAGLL
                     NWLLRARPSQAWAVGGNFATPWYPHPDQVKATVVYADGRLGSCDIYFGTPCRAPAWEI
                     EAVGTQGIIRVNQDVFEGTLYASDSRIVPFYRNQNDVILEAIRSFFESLSAGAPPDLD
                     MPEAIEIIRLCEGWKQSIETGTPVQLN"
     gene            3872..>4444
                     /locus_tag="PLT86_12980"
     CDS             3872..>4444
                     /locus_tag="PLT86_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37568.1"
                     /translation="MNASGPRHMRWLLCGFVLAALPGISAAQSLMQRAILQGQAAARD
                     SAQAHYLNAARLAAEGKYGPAIETMRKAIEFLPSEPRLRSDLASLILASGDKAGAVRE
                     LEALASQVPPYAPAVVQLGDLRLADGDTAGAVRAYQSVVGGVLPYALAYVRLGDVAQD
                     QGKRAEAVRMYQHATSIDSTLIEAWLSLGSI"
BASE COUNT          887 a         1412 c         1209 g          936 t
ORIGIN      
        1 cgaaatgcca aatggattgt ccgcgatacg ggtaaggaag cccgtgtatc ggactaagct
       61 cccgagaacg tcaacggcga ggttcctggt tggattcgcc cggacccaga gcgccagcga
      121 ggcggcaggt agcccgttgt caacgattga atatgccggt ttgccgccgg gcgcgtttgc
      181 gaaccagccg tcgtagttct cgtcggctcc ctggcacgca aggatatgcc gcacacactg
      241 ctcctcgacc tccacaaacc ccgtatcacc tgtagtgcgc aggagttcgt gcgcagcgat
      301 caactgcgcc gcattgtggt gaagcccctc gttccccaga tcccggcaga gcttccagtg
      361 ccgcaacgcg cggtcagtga attctgcgtt ccgaagaaca cgcccaatgt gggcaaaact
      421 cgccaccagc cacagatttc gtgcctctcc cgccgcaacg cggtcttttt cgtcaccgaa
      481 ccggttgtct gtttcgcttt ccggggtgtt ccagtggccg tagccggaga aaacccggtc
      541 ccaaagcttg ccggaaaccg gttcctgcat cttccgaaga aactcggctc cccagagcgc
      601 ctcgtccaag atatcgcagt gtggtccgtt tcgtatggtt ctgcaaagat cagcgcgctg
      661 ctcatgcgtg tagagaagcg cccagacgga atgaggtgtg aacccattgt atttgttgta
      721 atccccggcg tcgtgccagc cgccagccac atctctcgtg tatccgtctg ccgtagtgat
      781 ccggtcatcc agatggcacg cctcgtgcca gccgggaatc gccattccgc acctttggta
      841 ccagtagaag cgctgcgccg tatcgagcgt tcgctccgca acgaagttgt tccctaacgc
      901 aaaaaccggg gattccgctt ccagatctcc tacccgggct tcgatgtagt aattgttcgg
      961 gatgtcgaga cgtgaaaaat cgccccgcca gtaggtgcgt tcccattcac caatccgccc
     1021 ggcgggttcc agagcgcctt caaacaccgt aacgaattcc ttacccggtc ccgcgattgg
     1081 agctatgacg cgaaaggacc ctcctacctc caacggtgcg ttacactgga cggtaaaggt
     1141 ttttggaagt cccacgttgt acccgacctg gttgacgaga acctgtaact cgggctgcga
     1201 ctcaccacga cattcggtta tctcgagagc cgccaatcgc tcatttccca gctcaacgac
     1261 gaaccgaaaa ctcgcggtgg ttcttctcgc gcctgcctgc gaaaggagga aacgcccgtc
     1321 cccttcagtc gcggcccaga ccggtggata gaacttgcct cgcttcgcat ggcaccattc
     1381 gatgccaatc ctgcctccgc gcacggttcc cgcgacggac acctggaggt tggcgtggtc
     1441 gagaggaatc ggtacggggg aggatatcca gatggttccg gaatctcccg caagcgtaac
     1501 tggattccct gcacattccg cggcagggtc atgcatgttc ctgatccatg aatcgcgagg
     1561 catccatttc aggttgccca accaacgcgt gtaatcgcgg ttcactacca tcctgttcgc
     1621 tctcccgggt gtttcgaaaa tctcaccggt ccatttcaag aatcaccttc acgcaccctt
     1681 cccgcctgtc tgcgaaaagg tcatacgctt cctgaaccct ggtgaggggg aacgcgtgcg
     1741 tgagaatcgg ctttacgtcg acgcgtttct ccacaatgaa ctgcagcgcg agcgcgatat
     1801 ctcgctctac gtctggaacg tggtacgtaa ccatctgggc acgttttttg taccatgcga
     1861 acaggtcaag ctccgaacgc tggtacttgg gaaccccgaa catgacaatc cgcccgtcct
     1921 gtctgaccag ttcgatcatg agccgttgag tttcctcgta tcccgcagct tcgatcacga
     1981 gatcggcgcc cttgcctccc gtgagcctgg acactgtttc ccacggctgc cgggcagtcg
     2041 catcgactac cgccgtggca cccatcaacg ggcttatcct gaggcgttca ggaaccgagt
     2101 caataccgat tacctgcttc gccccgagat tccagagaat ccggtcgaac aactggccca
     2161 cgggcccctg gccgatcacc gccactgact gccccagcac cgacccgatt tcacgggtag
     2221 tccgcaacac cgttccgagc aattgcgcga gcaccagttc ggaaagattg ccctccgcag
     2281 gcagtttcag gagctggtga gcgtccgccg caacatactc gcgtagcccg ttctcgacag
     2341 gcggcgggaa atacaatacc cggtctccag gctcgaaacc gtcaagaaac gacttttcca
     2401 ccacaccggc gcattcgtgg cacggactgc caagttcgcc cgggtaccgc cgaggttgag
     2461 tttccagaaa cgtgatcaag tcacttccac aaatcgcgtt ccgcagaagc ctcacaagta
     2521 cctgtccctg cgaaatctcc ggaacctcga tttcgacgat ctccattttt cgcggagcca
     2581 ccatccggac tgcccgcatg tagtactccc tcccggccgt ggtgccaatc agttcaattg
     2641 caccggtgtg cccgtctcaa ttgactgctt ccatccctca cagagacgta taatttcgat
     2701 agcttcgggc atgtcgaggt caggtggggc cccggccgac agcgattcga aaaagctgcg
     2761 tatcgcctcg agtatgacat cgttctggtt tcgatagaac ggaacaatcc gcgagtcgga
     2821 cgcataaagc gttccctcga acacgtcttg attgacgcgg atgattccct gcgttccgac
     2881 ggcttcaatt tcccatgccg gggcacggca aggcgttcca aagtagatgt cgcacgaacc
     2941 cagccgcccg tctgcgtaca ctacggtagc cttcacctgg tcagggtgag ggtaccacgg
     3001 tgtcgcaaaa ttccctccaa ccgcccacgc ctgtgaaggt cgcgctcgca ggagccagtt
     3061 gaggagccct gcggcgtata cgccgagggt gaacagcgtc ccgccggagc gcggcgacat
     3121 ttccacgctt tcagagagat ccattttccc cggttgtccg tgctggatcc agcaccgcgc
     3181 gctgataacc tcaccaattt ctccgttcac cacccgtgtg cgcgcctccc gaatcgcgcc
     3241 gtcgtaacgg cccggttcga gcgtgctgat cgttctgccc gtttcccggc cgatttcacg
     3301 cagggcctga gcatcaaccg tgcttaagca caatggtttg gcaacgtaca catggcatcc
     3361 ttcccgaagc gccagttccg cgtacttccg tttctccgca tcgtgcgcgc acacaaacac
     3421 gacatcgagg ttttcgtgat gaatcatatc acgtgcggat tcgtaaagat tcagcgcgaa
     3481 ctcgtccgcg aagccctccc gggtataccc cgtgtaccgc ttcacgcttt ccgaactctg
     3541 gcccagatgc gcagccgcga tcagctttgc atggcgggag gttgcctgaa cggcccgcgc
     3601 aaacgcttgg gaataccatg agccaatccc aacaatgcca cagcggaaca ccatatcgat
     3661 acctcgcatt ctggaggtga aagccgatgt cagtgtgtga aagtagcgtt tcattgagga
     3721 agtatccaag cggcattcaa ggctgcggcc cgccgttcag gcccccttcg caagcatttc
     3781 ggtcttttcg tatctttatg tctcttgcgc ttccgtcggc catcttgcgg gcgttgtctg
     3841 cgtccgcctg caccgtaccg gggaatactc tatgaatgct tcgggccccc gccacatgcg
     3901 ctggctgttg tgtggttttg ttctggccgc ccttccgggt atcagcgccg cgcaatcgct
     3961 tatgcagcgg gcgatcctgc agggacaggc cgccgcgcgt gacagcgcgc aggcgcatta
     4021 cctgaacgcg gcgcgccttg cggcagaagg aaaatacggg cctgccatcg agacaatgcg
     4081 gaaggccatc gaatttctgc cgagcgaacc cagactgcgc tcagatcttg ccagcctcat
     4141 tcttgccagc ggagacaaag ctggagcagt gcgcgaactc gaggcacttg cttcgcaggt
     4201 tccgccgtac gcaccagccg tcgttcaact gggagatctc cggttggcgg acggcgatac
     4261 ggcaggagcc gttcgagcct atcagagcgt ggtaggaggc gtgcttccgt atgcgctggc
     4321 ctacgttcgt ctcggtgacg tcgcgcagga tcaggggaag cgcgcggaag cagtaaggat
     4381 gtaccagcac gcaaccagca tagattccac gctcattgag gcgtggttga gccttggctc
     4441 catt
//
LOCUS       DAOSOO010000241         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_19728_length_4436_cov_5.682493, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000241 DAOSOO010000000
VERSION     DAOSOO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19728_length_4436_cov_5.682493"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLT86_12985"
     CDS             complement(<1..277)
                     /locus_tag="PLT86_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT37569.1"
                     /translation="MVELVRTLRALVMIIRPANVVLTALSVAVGASCSSAPAGITLAA
                     ACACAALIAAGGYVVNDCFDVETDRINRPYRPIPSGALSHRTAVSWAV"
     gene            complement(367..2757)
                     /locus_tag="PLT86_12990"
     CDS             complement(367..2757)
                     /locus_tag="PLT86_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37570.1"
                     /translation="MHLRHIAALVLCVSAVVLAADATEELPLLPPLRIPPVLTSAFCE
                     YRPLRFHAGIDYSTGRRKGLPIQAIADGYVWRVRVSPFGYGKVLYFRLNDGRVAVFAH
                     LSRFTPRVEELLLEKQIANEQFVADVFFEPGEAPAKRGEVIAYSGDTGAGPPHLHFEL
                     RNERNIALNPLLQGYAFPDNIPPRVFAVAFIPLDGHARINGKPESANVKLVYRNGMWT
                     IPRVLRLSGRVGVTIAAADQVQRTGNIVGIFGTTLAVDGQEVFRREYDTLEMDYQFHA
                     PFDRSFIVNRVTKGRYYNLFKLPGNRLSFYGDAPAGAGILQCGTGSTQSPGTSLARGL
                     HEATITVSDAAGNATRVRFTFRVDRNPEFVSFALRDSAGVKVFSATAEDSGDVVRVFP
                     FGGEKGSKAIEGRDHVVWTTALRNPAAPIRVVVEDSAGNRSVQSISPNDFATVPRLRE
                     EHSWGRDWLLVSLRASRPLKGIPSAVALRAPVAATKLEPAALSAALSRPEVLPDPEWP
                     SPDFRGRWENSTDGTYAITERAANDTLPVFCELVANLTYRLTLPLTSLGGDVDTVRIL
                     ADGAEINIPVQGRLFTPMGGVYSHAGGACSVMCPPGALGEPVFVRVQETTLHRPPEPE
                     LRQVGKAFHLEPQEQPLADEVHVEIRIPEGEDTSGVALYGTTNGTLRFLSAEKDSTGR
                     YVRGRTRLFSTVCLFRDTAPPRLVVLSPSPNGTIRNGLVTVSGHVEDRGSGLDPGRDP
                     SEVRLDGKWVPAVPDASGHFAYRSTTPLPSGRHTVSIKARDLAGNVRHVSFEFRVP"
     gene            complement(2836..3996)
                     /gene="hemW"
                     /locus_tag="PLT86_12995"
     CDS             complement(2836..3996)
                     /gene="hemW"
                     /locus_tag="PLT86_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT37571.1"
                     /translation="MTPTSRSSRPAFRHIYVHIPFCVRKCPYCDFYSIAGHADRTTFL
                     RALESEIRIRNEQFGPFADIQTLYVGGGTPSCLDATELDALGTILRSAVQFAPDYEWS
                     MEANPESLSPSKAEQIRAMGVTRVSLGVQSLTHGVLSFLGRPHTAAEARSALRLLAEA
                     GFRSWNADLMFGIAGQSLEMLVDTLREVLAHDPPHLSAYALTIEPGSRCAEMADSARF
                     CTDENATARQYEYVVAALESVGYAQYEVSNFARSGHECRHNRAYWARRPYLGFGPSAH
                     SFDGHARWWNVCDLETYQARCVRGETPTAEMEVLTPREICEEIVLLSLRTREGLRWDA
                     LPDACPADARAALKPLQVAGLLDVDATGVRLSRHGFVVGDEVIRRVVELVCV"
     gene            complement(3993..>4436)
                     /locus_tag="PLT86_13000"
     CDS             complement(3993..>4436)
                     /locus_tag="PLT86_13000"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT37572.1"
                     /translation="VVLVVSARGVRNVLLNKTAPPATVDEVLQSKRLAGHAGALCELI
                     KGTRRDFPFPLDLTQGTDFQRRVWRALLAVPWGHVVTYGELAQRMGLPVGASRAIGQA
                     NAANPLPILIPCHRVVAAGGRLGGYSGGLETKRFLLRREGITPPR"
BASE COUNT          878 a         1404 c         1343 g          811 t
ORIGIN      
        1 ttaccgccca cgaaacggcg gttcgatggc tgagcgctcc gctcgggatc ggacggtatg
       61 gtcggtttat ccggtcggtt tccacgtcga agcagtcatt caccacgtat ccaccggcag
      121 cgatcagcgc agcacacgcg cacgcggctg cgagggtgat gcctgccggc gcggaagaac
      181 atgaagcgcc gactgcaacg gaaagcgccg tgagaacgac gttcgccggc cgtatgatca
      241 ttaccagagc gcgtagagtg cgaaccagtt ccaccacgga tggtgtcccg tctcaaaaaa
      301 cctgaaaaca accgcgataa gtgcgggaaa tgtaaaaacc ccgttctccc gacaccagtc
      361 tcctcctcac gggaccctga attcgaagct cacgtgccga acgttgccgg cgaggtcacg
      421 cgctttgatg gagaccgtat gacggcctga gggaagcgga gttgtggatc ggtacgcaaa
      481 atggccgctc gcatccggta cagccggaac ccatttccca tcgagacgca cctcggaggg
      541 gtcgcggccc gggtcaagac ctgatccccg gtcctcgacg tggccagata ccgtgacgag
      601 gccattcctg atcgttccgt tcggagacgg ggacaacacg acaagccgcg gcggtgctgt
      661 gtcgcggaag agacacactg ttgagaacaa ccgcgtacgt ccgcgcacgt acctgcccgt
      721 cgagtctttt tccgcgctca gaaaccgcag ggtgccgttc gtcgttccgt agagcgccac
      781 tcctgaggtg tcctcccctt ccggaatacg gatttcaaca tgcacttcgt ccgccagcgg
      841 ttgctcctga ggttcgaggt ggaacgcctt tcccacctgc cggagttctg gttcgggagg
      901 ccggtgcagc gtggtctcct ggacccgcac gaacaccggt tccccgagcg ctcccggagg
      961 gcacattacg gaacacgcac ccccggcatg ggagtatact ccccccatgg gagtgaaaag
     1021 cctgccttgc acgggaatgt tgatctccgc gccatcagca agaattctga ctgtgtcgac
     1081 gtctccgcca agtgaggtga gtggtagtgt gagcctgtac gtcaaattgg cgaccaactc
     1141 gcaaaaaacg ggcagggtgt cgtttgccgc gcgttcggtg atcgcgtagg ttccatcggt
     1201 gctgttctcc catctaccgc ggaaatcggg cgatggccat tctggatccg gcagaacctc
     1261 cggtcgggag agtgcggccg aaagtgctgc tggctcgagc ttcgttgcgg cgaccggtgc
     1321 tcgaagtgcc accgccgagg ggataccctt caaggggcgt gaggccctga ggctcacgag
     1381 cagccagtct cgtccccacg agtgctcttc acggagccgc ggtacggtcg cgaaatcgtt
     1441 cggggagatt gactgcacgg agcgatttcc cgcggaatcc tccaccacaa cgcggatcgg
     1501 tgccgccgga tttctgaggg cagttgtcca caccacatgg tctcggcctt cgattgcctt
     1561 tgaacccttt tctccgccga acgggaacac tcggacaacg tcgcctgaat cctctgctgt
     1621 tgccgaaaag accttgacac ccgcagaatc gcgcagggca aaggaaacaa actccggatt
     1681 cctgtcaacg cggaaggtga agcgcacgcg ggtggcgttc ccggcggcat cggataccgt
     1741 aatcgtggct tcgtgaaggc ccctcgcgag tgaggtgccc gggctctgcg tcgaaccggt
     1801 tccacactgg agaatacccg ctccggccgg ggcatccccg tagaagctca acctgttccc
     1861 tgggagcttg aagaggttgt aataccggcc cttcgtcacc cggttgacaa tgaagctcct
     1921 gtcgaagggc gcatgaaact ggtaatccat ctcgagggtg tcgtactcgc ggcgaaacac
     1981 ctcctgtccg tcaacggcca acgtggttcc gaagatgccg acgatgtttc cggtgcgctg
     2041 gacctggtca gccgccgcga tggtcacccc gactctcccg ctgagcctga gaacccgggg
     2101 tattgtccac attccgttgc ggtacacaag cttcacgttg gcggactcgg gcttcccgtt
     2161 aatgcgtgcg tgtccgtcca gtggaatgaa cgccacggcg aacacgcgag gcgggatatt
     2221 gtccggaaac gcgtacccct gaagaagcgg attgagggcg atgtttcgct cgtttctcag
     2281 ctcaaaatgg aggtgcggcg ggccggcgcc cgtgtcgccg gaatacgcga tcacttcacc
     2341 ccgttttgcc ggcgcttcac ccggttcgaa aaagacgtcc gcgacgaact gctcgttcgc
     2401 tatctgtttc tcgaggagaa gctcttccac gcgcggtgtg aagcggctca gatgggcaaa
     2461 taccgcgacc ctcccgtcat tgagccggaa gtagagaacc tttccgtatc cgaaaggaga
     2521 gacccgcacc cgccagacat atccgtctgc aatcgcctgt atcggcagcc ccttacggcg
     2581 tcccgtggag tagtctattc ccgcgtgaaa acgaagaggg cggtactcgc agaaggcaga
     2641 ggtcagaacg ggagggattc ggagcggagg aagcagggga agttcttcag tcgcatcggc
     2701 tgccagcacg accgcagaca cgcacagaac cagggcggcg atatgtctca ggtgcacaat
     2761 ttctctccat ggaatccccc tcgcacaacg aaccaacctt ggtgaaaggt acggggaaac
     2821 gcgcaccgtg gccaatcaga cgcacacgag ttcaaccacc cggcggatta cctcatcccc
     2881 gacgacgaac ccgtgtcgcg aaaggcggac tcccgttgca tcgacgtcca acaaccccgc
     2941 cacctgcaac ggcttcagtg ccgcgcgtgc atccgcagga cacgcatcag gcaaggcatc
     3001 ccagcggaga ccctctcgcg tgcgaaggct cagaagcacg atctcctcac atatttcgcg
     3061 cggcgtgagc acctccatct ccgcggtcgg cgtctcgcct cgtacgcacc gggcctgata
     3121 cgtttcgaga tcgcacacgt tccaccaccg cgcgtgcccg tcgaacgagt gcgccgaggg
     3181 accgaacccg aggtacggcc gccgtgccca gtatgctcgg ttgtggcggc attcgtgccc
     3241 ggatctcgcg aaattcgata cctcgtactg cgcgtaccct accgattcaa gcgccgcaac
     3301 gacgtactcg tactgccggg cggtcgcgtt ctcgtcggtg cagaatcgcg ccgagtcagc
     3361 catttccgcg cacctgcttc ccggttcgat cgtcagcgca tatgccgaca ggtgaggcgg
     3421 gtcgtgtgca agcacttcac gcagcgtatc caccagcatt tcaagcgact gccccgcgat
     3481 gccgaacatc aggtcggcgt tccacgagcg gaaccccgcc tctgccagaa gcctcagcgc
     3541 ggaacgcgct tctgccgcgg tgtgcggccg cccgaggaaa gagaggaccc cgtgcgtgag
     3601 agactgcacg ccgagggaca cccgggtcac tcccatcgcg cgtatctgtt ccgccttcga
     3661 aggggagagc gattccggat ttgcctccat gctccactcg taatcaggcg cgaattgcac
     3721 tgccgaccgg agaatggtgc ccagagcatc cagttcggtt gcgtcgaggc aactcggcgt
     3781 cccgccgccg acgtacaacg tctggatgtc ggcgaatggg ccgaattgct cgttccggat
     3841 gcggatttcc gattcaagcg cccggagaaa agtcgtccgg tctgcgtggc ctgcgatgga
     3901 gtagaaatcg cagtaggggc atttgcggac gcagaacgga atgtggacgt agatgtgacg
     3961 gaacgccggg cgggaacttc tcgacgttgg tgtcatcgag gcggagtgat cccttcacgt
     4021 cgaaggagaa aacgtttcgt ttcaagtcct ccggagtaac ccccgagtct gcctccggcc
     4081 gccaccaccc ggtggcacgg gatgaggatg ggcaacggat tcgccgcgtt cgcctgtccg
     4141 attgccctgc tggctcccac ggggagtccc atcctctgcg caagttcgcc gtacgtcacc
     4201 acgtgtcccc acgggaccgc aagaagtgcc cgccacacgc ggcgctggaa atcggtcccc
     4261 tgggtcaagt cgagcggaaa cgggaagtcg cgccgcgtgc ccttgatgag ttcgcacagc
     4321 gcgcctgcgt gtccggcaag cctcttgctc tggagaactt catccacggt ggcgggcggc
     4381 gccgtcttat tcagcaacac attccgcacg ccgcgagccg atacaacaag gacgac
//
LOCUS       DAOSOO010000242         4375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_20069_length_4375_cov_4.322454, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000242 DAOSOO010000000
VERSION     DAOSOO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20069_length_4375_cov_4.322454"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..2321)
                     /locus_tag="PLT86_13005"
     CDS             complement(120..2321)
                     /locus_tag="PLT86_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT37573.1"
                     /translation="MVPQKSLIANGNDAVAQAIVDIGYDGEGYYPITPSSDAGERVSE
                     ALAAGKTDINFVIGTSELAVAGIITGMAVAGGRAVDVTSANGLLLKMEELPCISGLGL
                     PLVMNIATRDVSGPLNIKNGHSDLAAVLGVGWLIFLAPTVQATYDLNLIALKAAEAVS
                     LPAFVAYDGFHTSHGVRRINVFASQEDARAFVGKKPEMLNLLDVRHPHTFGPYMNDDL
                     INTKVQLDAKMKKAYELLPGIFKEFEELTGRHYSFVESYGSDNPDAVLVALNSAGEAA
                     KVAVDDMIADGKKVRVVIPTVLRPYPNDEFVDAVKGAKTIVVAERVSQYGAGNYLANE
                     LGASLQRIGSKSTILSRVYGIGGLNYLPEDGVHMLTTALAYPNVPEEEKKIHAYHGAW
                     PGDPSYKPLPTIQPLTAEETTLNAGEERVNLRKLAEMPTRIEKHSACPGCGIFTNLNL
                     FLRGIDGHVVLLFNTGCGMVVTTGYPLTSFKVPYFHNLFHNGSSTATGVVEMYNRFRK
                     QGKINEEITFIVVSGDGGDDIGMDQVIGSALRNDPFIMLEYDNKGYMNTGGQLCYTGF
                     KGQRNSNAPIGPALRGKKTHHKDVIEILRGTECPYLFQAAESNARDMIAKARKAQATV
                     RAGGFAFGKVFSVCPLNWGADEAKGNEIVEKVVDACMHPLYEVEHGVTRLSYDPEERG
                     KKIPVGDAFRAMGRAFGHLLKPDNADLLQEVQDEVDRRWERLKAMSSSEIL"
     gene            complement(2385..3326)
                     /locus_tag="PLT86_13010"
     CDS             complement(2385..3326)
                     /locus_tag="PLT86_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT37574.1"
                     /translation="MSYQLPAIDRLGFCNVFLSAIGGDGANMSAKLLFKIAVTELDLD
                     GAYDAKYGSEKKGTATDVSLRLCYKGTAVRTSGPTTTPHILAIFRPGLIETLQLNRGL
                     QANATVIVNTTQTPDEIRDRLKMHSGKIVCVDAETIAEETGSRLNMPMMALVCRALHF
                     PQEQVEAVIAKTWPYAKDANLGAFKAAYDGVNEKEFAPRADYPLLPPALVARGQIGYL
                     NMLNGGAIDAMTHSTVNRNNQVAGYGFVPTFDPSACTGCAVCMTVCSDPGGIVWKDGK
                     VIGIDYAFCKGCMRCVEVCPQTKKGKALNRPEIAQVA"
     gene            4007..>4375
                     /locus_tag="PLT86_13015"
     CDS             4007..>4375
                     /locus_tag="PLT86_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT37575.1"
                     /translation="MLRGEFTPLDGFKGPGYHAAVALVSFVARDLFLSAKIVALASSA
                     IVLWLLFRLVRRSLGTGTAWVAMLFVATNAQFILFTIQVGTDMFFLAVALAAATLILE
                     RGGWQRSLAAGLMAGFAYLTR"
BASE COUNT          830 a         1288 c         1236 g         1021 t
ORIGIN      
        1 gcgagtgcct gctcgccgat ctatgcccct cagccaagcc ctgatttccc agccttcaca
       61 aaacaagacg cctcggggat aatggttccc cgaagcgtct tgccttttcg ttgctgtttt
      121 tacaggattt ctgatgaact catcgccttg agacgctccc agcgacgatc cacctcgtcc
      181 tgaacctcct gcagcagatc cgcgttatcg ggcttgagca agtgcccaaa cgcgcgtccc
      241 atcgcgcgga acgcgtcgcc gacggggatc ttcttgcctc gctcttccgg gtcgtaactt
      301 agtcgtgtga caccgtgctc gacctcgtac agcgggtgca tgcacgcatc caccaccttt
      361 tcgacaatct cattaccctt cgcctcatcc gctccccagt tcaacgggca gacgctgaac
      421 accttgccaa atgcgaaacc accggcacgc acagttgcct gcgctttacg ggccttcgca
      481 atcatgtcgc gggcgttcga ctcagccgcc tggaaaaggt aggggcattc cgttccgcgg
      541 agtatttcga tcacgtcctt gtgatgcgtc ttcttaccgc gaagggcggg cccgatcggg
      601 gcgttggagt tccgttggcc cttaaacccg gtgtagcaga gctgcccgcc ggtgttcatg
      661 tagcctttgt tgtcgtactc cagcatgatg aacgggtcgt tacgaagcgc tgaaccgatc
      721 acctggtcca ttccgatgtc atcgccaccg tcgcctgaca cgacgatgaa ggtgatctcc
      781 tcgttgatct tcccctgctt gcggaatcga ttgtacatct ccaccacgcc ggttgcagtg
      841 gaggaaccgt tgtggaagag gttgtggaag tagggaacct tgaagctggt aagcgggtac
      901 cccgtcgtaa ccaccatccc gcaaccggta ttgaacagaa ggactacatg cccgtctatt
      961 ccgcgaagga acaggttcag attggtgaaa atcccacatc ccgggcacgc cgagtgtttc
     1021 tcgatccgcg tcggcatctc ggcaagtttg cggaggttca cgcgttcctc acccgcgttc
     1081 agcgtggtct cctctgcggt gagaggctga atcgtcggga gcggtttgta actgggatcg
     1141 cccggccacg caccatggta tgcgtgaatt ttcttttctt cttccggaac gttcgggtag
     1201 gcgagcgccg tggtgagcat gtgtacgccg tcttcgggaa ggtaattcag acctccgatg
     1261 ccgtacaccc ggctcaaaat cgtgctcttc gagccaattc tctgcaatga cgcgcccagt
     1321 tcattcgcca gataattgcc tgccccatat tgcgaaacgc gttcggccac aacaatcgtc
     1381 ttcgcacctt tgaccgcatc cacgaattcg tcgttgggat aaggccgcag aacggtcggg
     1441 atgaccaccc tgaccttttt gccgtccgca atcatgtcgt caacagccac tttggctgct
     1501 tctccggcac tgttcagggc cacaagcacc gcgtccgggt tgtcagagcc gtagctttcc
     1561 acgaagctgt agtgccgccc tgtgagctcc tcgaattcct tgaagatgcc gggcagaagc
     1621 tcgtacgctt tcttcatctt cgcgtcaagc tggaccttcg tgttgatgag gtcgtcgttc
     1681 atgtagggcc cgaacgtgtg cggatgacgt acatccagca ggttcagcat ttcgggtttt
     1741 ttcccgacga acgctcgcgc gtcttcctgg ctggcgaaca cattgatgcg gcgtacgcca
     1801 tggcttgtat ggaagccatc gtacgccaca aacgccggca gagaaactgc ctcggcagcc
     1861 ttcagcgcga tcaggttcag gtcatacgtg gcctgaacgg tcggcgcgag gaaaatcagc
     1921 cagccaacgc ccagcactgc tgcaaggtcg ctgtgtccat ttttgatgtt cagagggcca
     1981 ctgacgtccc tggttgcgat attcatgacc agcggcaggc cgagtccgct gatgcaggga
     2041 agttcctcca ttttcaggag aagcccgttc gcggaggtaa cgtcaacggc tctgccgccc
     2101 gcaaccgcca ttcccgtgat gatgccggcg accgccagtt ctgatgtacc tatgacgaaa
     2161 ttgatgtctg ttttgcccgc ggcaagtgcc tcgctgacgc gttcacctgc gtcagatgaa
     2221 ggtgtaatcg ggtagtagcc ctcaccgtca tagccaatgt ctacaatcgc ttgggcaaca
     2281 gcgtcgttgc cattcgcgat aagcgatttc tggggtacca tcgtgtgcca ctccctgttg
     2341 caggtcggag cagggatcaa gcctctccga gccggaaaga acgatcaggc tacttgcgca
     2401 atctctggac gattcagggc tttgcccttc ttggtctgcg gacatacctc aacacacctc
     2461 atgcacccct tgcagaatgc ataatcgatg ccgatcacct tgccgtcctt ccagacgatc
     2521 ccgcccgggt cagagcagac agtcatacac accgcgcagc cggtgcacgc cgaagggtcg
     2581 aatgtcggga caaacccgta cccggcaacc tggttgttcc ggttgaccgt ggaatgggtc
     2641 atcgcgtcaa tggcgccacc attgagcatg ttgaggtatc cgatctggcc acgcgcaacc
     2701 agggctggcg gaaggagcgg ataatcggcc cgaggagcga actctttttc gttgacgccg
     2761 tcatacgcgg ctttgaatgc cccgagattg gcatccttcg catagggcca tgttttggcg
     2821 atcacggcct ctacctgctc ttgggggaag tggagtgcgc ggcaaacgag cgccatcatc
     2881 ggcatgttga gccgcgagcc ggtttcttcc gcaatcgtct cggcgtcgac gcagacgatt
     2941 ttgcccgaat gcatcttgag gcggtcgcgg atttcgtccg gtgtctgcgt ggtgttcacg
     3001 atgaccgttg cattggcctg cagcccgcga ttcagttgga gtgtttcaat cagccccgga
     3061 cggaaaatcg caaggatgtg aggcgtggtg gtgggtccgg aggtccgaac ggctgtgcct
     3121 ttgtagcaaa ggcggagcga cacgtcggtt gcagtgcctt tcttttcgct cccgtatttc
     3181 gcgtcgtacg caccgtccag gtccagctcc gtcacagcga ttttgaacag caacttggcg
     3241 gacatgttcg cgccgtcacc accgatggcg gaaagaaaca cgttacagaa accgagccga
     3301 tctatcgccg gcaactggta tgacatggag gattcctcct gcggagaaaa gcatcaacgt
     3361 gtgatgcggt tgatgcgatg tgtgcccgac ggatcacgtc gggacgtgaa agcgcttcga
     3421 cgggtagatt gtacaatatc gcaaatcctg tgccgggcag aaacccctga agacggttga
     3481 aaacggcgta tcaggcgcca tatctcagca ttgcttcagc cggccttggc atgcggtgtc
     3541 ctgtttcgtt gcagccctgc tgccgtttga cgcggttcga cgtgtgcgct aagcaagccc
     3601 cgtttgtccc cttcccgcga tttcgtctcc ccgctcgtga agcagcctgt ggctcctgtg
     3661 cttggcgaac agcgtccgcg gtcgtacagt attttcgagc ctttccaaca agcacgcatg
     3721 ccggactttg ttgcgccagg aggtaaccgt ggcgaatcgc gtaccgcctt ccaaacacca
     3781 aaaaacgaga tccaaacctc tacctccccc cacagtgaaa aacaaacctg cgctatataa
     3841 tattgcgcca caactacctg cgtggtttgg ttctccattc attccggccg gggttattgt
     3901 agcatatgtc atcgtgtggg gcgttttcag cgttggattc tggagggtag gagggtacgg
     3961 agtcgaaacg gacgcgttcg ggagctttct ccccgaagtc aggaagatgt tgcggggcga
     4021 attcactccg ttggatgggt tcaaaggtcc gggatatcat gctgcggtag ctttggtgag
     4081 cttcgttgcg cgcgatctct tcctttccgc gaaaatcgtg gctcttgcat cttccgcaat
     4141 cgttctctgg ctgttgttcc gccttgtgcg gcgctcgctg ggaactggca ccgcgtgggt
     4201 agccatgttg ttcgttgcca cgaatgccca gttcattctc ttcacgatcc aggtcggcac
     4261 ggacatgttc ttcctcgccg tcgcgcttgc tgcggcaacc ctgattctgg agcgaggggg
     4321 gtggcaacga tctcttgcgg cgggtttgat ggccggattc gcctatctca cgcgg
//
LOCUS       DAOSOO010000243         4347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_20235_length_4347_cov_4.901678, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000243 DAOSOO010000000
VERSION     DAOSOO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20235_length_4347_cov_4.901678"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..920
                     /locus_tag="PLT86_13020"
     CDS             282..920
                     /locus_tag="PLT86_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOT37576.1"
                     /translation="MAQLQALADAIIAGNRVKTAELTAEALSAGMPAKQILDEGLIAG
                     MAVVGEKFKNCEFYVPEVLVAARAMAAAMEKLRPALVEAKVEPIGKVAIGTVRGDLHD
                     IGKNLVAMMLEGAGFEIIDLGVDCSPEQFVGAVKEKGANIVALSALLTTTMPAMRDTI
                     EAMTAAGVRDKAKVIIGGAPVTQKFADQIGADGYAADAASGVDVAKSALGIA"
     gene            1129..1536
                     /locus_tag="PLT86_13025"
     CDS             1129..1536
                     /locus_tag="PLT86_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT37577.1"
                     /translation="MFFNVKAKPWYIRLVDIGEQFAKVPLFDCSMCGQCVLRSTGLTC
                     PMRCPKQMRNGPCGGSMNGKCEVFPERPCIWNLIWERSEMLGWEEKLENINPPVDWRL
                     WGTPSWWNLLTGRIDGKGHAVEPKKESEPSKTN"
     gene            1615..2634
                     /locus_tag="PLT86_13030"
     CDS             1615..2634
                     /locus_tag="PLT86_13030"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT37578.1"
                     /translation="MDNKASDSQVSPVSRPCASTLERVLRDKKFAVTAEIVPPRVPNL
                     SVVGKKFELLREVADAINITDNASASVKICSLAVCAYLVQQGGEPVFQVTARDRNRLA
                     LQSDLIGAYALGVRNVFAVTGDYINMGDHPAGKPVYDMDSVHIIQMFDKIRHGYMDSG
                     VELRSTSKTPLVQPNFFLGAAANPFGDPMPFHVLKVAKKRLAGADFIQTQCTFDVPRV
                     REFMAMVVDKGFHERLHFLIGIMPVRSARPLEFMATEVPGMRVPAEAIARMKGAQNAE
                     EEGIQMAVETIQQVREIPGVSGIHLMTVSWEAVVPEVLKRAGLMPEQRGVVEIHAAAK
                     SGNAS"
     gene            2674..3474
                     /locus_tag="PLT86_13035"
     CDS             2674..3474
                     /locus_tag="PLT86_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HOT37579.1"
                     /translation="MLVVGERINSTRKRLLPAIETRDIAVITDEATMQVNAGAHYLDC
                     NAAAVGVEKEPEYLTWLVATVQQVSEDTPCAIDSPNPVAVEAALKVHKGVPMINSISA
                     EKAKYDGLMPLARDSKAKVVALLMDDAGIPKTAADRIAIGRSLVTSLVKDGIAADNIY
                     VDPLIYPIGAEASAGTAVLETISTLKKEFDGVHFICGLSNISYGLPVRKLLNQAFMVL
                     TIGAGLDAAIIDPTDRKLMSLAFAAEALVGADEYCANYIQAARQERLE"
     gene            complement(3540..>4347)
                     /locus_tag="PLT86_13040"
     CDS             complement(3540..>4347)
                     /locus_tag="PLT86_13040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT37580.1"
                     /translation="YESGDDPVNLTFYFRPPLCEEAVRSAFLESVPSHADGMVQVSFT
                     TETDRDWEQEWRRNLHPLRVGRTWLVHTSWQSPAGYPTRIPIVIDPKMAFGTGTHATT
                     RLCLEELERFPLRGTRVLDVGTGTGILAIAAARSGAENVIAVEIDHEAIACARENLSL
                     NGIGNTVTLREGTIAAVPEHGFDLIVANIELRTLIAVGEALRARIHPDGRAVFSGILQ
                     QEADLFVQAMTKVGWQTMRRTRKFDPATGDGWVCCVATPSFPAGAKSDLF"
BASE COUNT          944 a         1188 c         1329 g          886 t
ORIGIN      
        1 tattgtggtt gcatcgcacc cttgcgttct gcacgtgggt cctgtttttt ttacaaccgt
       61 tgaggggcgg tgttgcgttt tcgggtccca ttgacgttcg tacaatattc gagtttgcag
      121 gcagcactta ttccgggttt ccggggagtc cggagactgc cgaagggtgc cgggagtgcc
      181 tgacagggca gttcccgttt ttgaaacgaa caatccaggg cgcgttcgtg tgcgtggacg
      241 ggcgcgtgct gatttcaagc cgaaacgacg gaggcacgaa catggctcaa cttcaagcgc
      301 ttgctgatgc gattatcgcg ggcaaccgcg tcaaaacggc cgaactcacc gcggaagcgt
      361 tgagcgcagg aatgcctgcg aagcagattc ttgacgaggg gctgattgcg ggtatggccg
      421 tggttggcga gaagttcaaa aactgcgagt tctacgttcc cgaggtgttg gtcgccgcgc
      481 gggctatggc ggcggcgatg gaaaagcttc ggcctgcgct tgtggaagcc aaagtggaac
      541 cgataggcaa agttgcgatc ggtaccgtcc gtggcgacct gcatgacatc ggcaagaacc
      601 tcgtggcaat gatgcttgag ggtgccggtt ttgagattat cgacctcggc gttgattgtt
      661 cgcccgagca gtttgtcggt gccgtgaaag aaaagggtgc caacatagtt gccctttcgg
      721 ctctgctcac gacaacgatg cctgcgatgc gtgacaccat cgaagccatg acggccgccg
      781 gcgtgcggga caaggcaaag gtgattatcg gtggcgctcc ggtaacacag aagttcgccg
      841 atcagattgg cgcggacggt tatgctgcgg acgcggcgag cggcgtggac gttgccaaga
      901 gcgcgctcgg aatagcgtag ttccagttcc gcgcaaattt gccgacaaac cggatcgcaa
      961 gcgaaaacgc cggctgggtg cgtgtgttcc cagtcggcgc ttttgtgcgc gaacacctgc
     1021 agggttttcc ttccctttgg cggcgcatgc ttcgttcaat tgcctgcaac ggcagagagt
     1081 gcgtaaagta gcggacgaac gagcgggttc gaaaagagag ggtagacgat gtttttcaac
     1141 gtaaaagcga aaccgtggta catccgtctt gtggatattg gcgagcagtt tgccaaggtt
     1201 ccgctgttcg attgttccat gtgcgggcag tgcgttctga gatccaccgg actgacgtgc
     1261 cccatgcgtt gcccgaagca gatgcgaaac gggccctgcg gcggttcaat gaacggcaag
     1321 tgcgaagtat ttcccgaacg gccgtgcatc tggaacctca tctgggagcg ctccgagatg
     1381 cttggatggg aagaaaaact ggaaaacatc aacccacccg tcgactggcg actgtgggga
     1441 actccgtcat ggtggaacct gttgaccggc aggatcgacg ggaagggaca tgcagtggaa
     1501 ccgaaaaagg aatcagaacc ctcaaaaacg aattaaggcg gaacaacgac ataacccctg
     1561 acaccagttt ccgtatgccg gcgttcgttc cgattcccag gagcttcccg aaccatggat
     1621 aacaaagcat cagattcaca ggtctccccc gtttcccggc catgcgcgag cactctcgag
     1681 cgagtactgc gggataagaa gtttgccgtc acagcggaga ttgttccgcc tcgtgtgcct
     1741 aatctgtcgg tggtcggcaa gaaatttgag ctgcttcgtg aggtcgctga tgcgatcaac
     1801 atcaccgaca atgcgtcggc ctccgtcaag atctgcagcc ttgcggtctg tgcgtacctg
     1861 gtgcagcagg ggggcgaacc ggtatttcag gtgaccgcgc gggaccgcaa caggctggca
     1921 ctgcagtctg atctgattgg cgcgtacgcc ctgggcgtcc ggaacgtgtt tgcagtgacg
     1981 ggggactaca tcaacatggg agatcacccg gccggcaaac ccgtgtacga catggattcc
     2041 gtgcacatta tccagatgtt tgacaaaatt cggcatgggt acatggattc cggtgtggaa
     2101 cttcgttcca cttcgaaaac acccctcgtg caacccaact tttttcttgg agccgccgcg
     2161 aacccgtttg gcgatccgat gccattccac gttctcaaag ttgcaaaaaa gcgcctcgcg
     2221 ggggccgatt tcatccagac gcagtgcacg tttgatgtcc cgcgggtccg ggaattcatg
     2281 gcgatggtgg tggacaaagg attccacgag cggcttcatt tcctgatcgg aatcatgccc
     2341 gtgagaagcg cgcgtccttt ggagttcatg gccaccgagg tgcctggaat gagggttccg
     2401 gcggaagcga tagcccgcat gaaaggggcg cagaacgcgg aggaagaagg cattcaaatg
     2461 gccgtggaaa ccatccaaca ggtgcgcgaa atacccggcg tttccggtat tcacttgatg
     2521 accgtgagtt gggaagccgt cgttccggaa gtattgaaac gtgcgggtct catgcctgag
     2581 caacgcggcg tagtggagat tcacgctgcc gcgaagtcag gaaatgcttc ctgaagactc
     2641 gggaacacac aacaaagacg ggagaggaca acgatgctag ttgtcggaga acgcatcaac
     2701 agcacacgca aacgccttct gccagcaatc gaaacgcggg atatcgcagt tatcacagac
     2761 gaagcgacga tgcaggtcaa tgcgggtgcg cactacctgg attgcaacgc agcggccgtg
     2821 ggcgtggaga aggagccgga ataccttacg tggctggtcg ccacggttca acaggtgtcg
     2881 gaggacacgc cctgcgccat cgacagcccg aacccagttg cggtggaggc ggcgctcaag
     2941 gtacacaagg gggtgccgat gatcaactct atcagcgccg aaaaggcgaa gtacgacggg
     3001 ttgatgccac tggcgcgcga ttcaaaagcc aaggtcgtgg ctctgctgat ggacgacgca
     3061 ggaataccga aaacggccgc ggacaggatc gcgatcggca gatcattggt caccagcctc
     3121 gtgaaagacg gtattgccgc agacaatatc tacgttgacc cgctcattta ccccatcggt
     3181 gcggaggcaa gcgcaggaac agcggtgctg gaaaccatca gcacgctgaa gaaggagttt
     3241 gatggagtcc atttcatctg cggattgagc aacatctcct acggtcttcc ggttcgcaaa
     3301 ttactcaatc aggcgttcat ggtacttacc atcggcgccg gacttgacgc agcgatcatt
     3361 gacccgacag accgcaaact gatgtcgctg gcgtttgccg ccgaggccct cgtcggtgca
     3421 gacgaatact gcgccaatta catccaggca gcacgccagg agcgtctgga atagggtctt
     3481 ccccttcggt tcgcagggtg cgcaagttcg atacgtgcgc gccctgcgcc agaaatcttt
     3541 cagaaaaggt ctgatttcgc acccgcgggg aacgacggcg tggcaacaca gcagacccac
     3601 ccgtcgcccg tcgcagggtc aaatttccgc gtgcggcgca tcgtttgcca tcccaccttc
     3661 gtcatcgcct ggacgaacaa atctgcttcc tgttggagga tcccgctgaa taccgccctt
     3721 ccgtcgggat ggattcgggc ccgcaatgcc tctcccactg cgatgagcgt gcggagctca
     3781 atgttggcga caatcaggtc aaaaccgtgt tcgggaaccg cggcgattgt tccttcccta
     3841 agcgttacgg tgttccctat cccgttgagg ctgaggtttt cccgagcgca cgcgatcgcc
     3901 tcatggtcga tttccactgc gatcacattc tctgcgccgg acctggcggc ggcgatggcg
     3961 agaatgccgg ttccggtgcc cacatcgagc acgcgcgtac ctctcagagg aaaccgctca
     4021 agctcctcta ggcaaagccg cgtggttgcg tgagttccgg taccaaacgc catcttcgga
     4081 tctatgacga tcggtatccg ggtcgggtat cctgctggcg attgccaaga ggtgtgcacc
     4141 aaccacgtcc tacccacccg cagaggatga agattgcgcc tccactcctg ttcccagtcg
     4201 cggtccgttt ccgtcgtgaa cgatacctgc accatgccgt cggcgtggga cggtaccgac
     4261 tcgagaaagg cggagcgaac cgcttcttcg caaagtggcg gacggaagta gaacgtgaga
     4321 ttgaccgggt cgtccccgga ttcatat
//
LOCUS       DAOSOO010000244         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21530_length_4153_cov_5.680820, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000244 DAOSOO010000000
VERSION     DAOSOO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21530_length_4153_cov_5.680820"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLT86_13045"
     CDS             complement(<1..940)
                     /locus_tag="PLT86_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37581.1"
                     /translation="MPTPSRPNILLLMTDQQRYDSLGCYGFPAAHTPNLDRLAREGAL
                     FEKFYVTNPICTPSRSSMFTGKHLHGHGVYTVYGNLPEDEVLFPEHLQRAGYETALFG
                     KLHIRSRMLENSERHPHDGFDVYEWALEACLDLDSPLNGYGKWLKERDPVFFKKLKRE
                     GRKLLHHPAHLHFTHWAAERTIGYLKRQDGSRPFFAMMSIFDPHNPYEAWPEEFGRLV
                     DAGKIPDPLVTEGEMERLPRGIRQEHEGSYLGAFSRYTMDDLRRMRFGYHVSIAFADH
                     EFGRVLDTLREQGLEENTLVIFASDHGDMLGDHSLLV"
     gene            1131..2033
                     /locus_tag="PLT86_13050"
     CDS             1131..2033
                     /locus_tag="PLT86_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37582.1"
                     /translation="MDNVLLKTSELSVVVGNNEPGSGDFAAHRAGYNGIWSLTSLHSP
                     ENCFVPFYAGLNLEHCMDDLFMTEEGVDNFEPRHAPMELKQLSGGSACLYQPLTPLTG
                     VESETVFEVKEPYVIDMRFAATLHRPPRVGRWFGFFWASYVNAPDSPALYFRDADGML
                     NAMAPDVHGEGGANTVCHASVEDPALGDPGRRYAGHSLAHSWSRRRFGAPLMFGRPGD
                     GRMLYLQMFDQQSPVRITMSPSGGGRNEEKKAFNPAWDFQYVRGEATTGTEIRLRSRI
                     VYKQYDGLEEIDRFYTEWEQHVAG"
     gene            complement(2090..2317)
                     /locus_tag="PLT86_13055"
     CDS             complement(2090..2317)
                     /locus_tag="PLT86_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37583.1"
                     /translation="MRLFSCLFVPILCFAGTGVAAAPTAESAVPAAVLAHNAGHVVLR
                     SWLVGGMFPSTPLAQPQGGDTRLEGHTQKAV"
     gene            2572..3156
                     /locus_tag="PLT86_13060"
     CDS             2572..3156
                     /locus_tag="PLT86_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37584.1"
                     /translation="MGLLSLVILLASAYATARYNVFGDVAWSEWPTLVLNKILALSAL
                     ALLAIHTIRRRGVQETSARTIVGVAGFLAAVHVLLSLVLLSPAYYGKLFNLDRLTASA
                     GIAMLLGALAAATLAVGGKLLVGPDTREPRRYFAFISFVIGLHAAFQGFFGWFTPWNW
                     PGGMPPISLLSFALGIVALVAGLRRGESPSRSNR"
     gene            3176..4078
                     /locus_tag="PLT86_13065"
     CDS             3176..4078
                     /locus_tag="PLT86_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT37585.1"
                     /translation="MAETRNIRKVLRARPTLEGAGVHLNRVFGGEHVPQFDPFLLLDD
                     FSSNNPAEYIKGFPWHPHRGIETITYVLAGEVEHGDSMGNSGVISSGDVQWMTAGGGI
                     IHQEMPKGDAQGVLRGFQLWANLPAANKMMPPRYRGIQAEQIPQVALPGGATARIICG
                     EVAGAKGPVGDVVIEPLYMDITVPAGGELHFPVPKGHTVMAYVIDGKGQFAPESDPFA
                     FDVEGRNYFDLERECQIGTRNLVVFGEGDELAIAGGDAGVRFLLIAGKPIGEPVAWHG
                     PIVMNTQAELRQAISDLQNGTFIR"
BASE COUNT          782 a         1177 c         1383 g          811 t
ORIGIN      
        1 tcaccagcag gctgtggtcg cccagcatgt cgccgtgatc ggacgcgaag atcacgaggg
       61 tgttctcttc gaggccctgt tccctcaggg tgtcgagcac tcggccgaac tcgtggtcgg
      121 cgaatgcgat ggacacgtgg tacccgaaac gcatcctccg gaggtcgtcc atggtgtagc
      181 gagagaacgc gccgaggtag ctgccctcgt gctcctgccg gatgccgcgg ggcaggcgtt
      241 ccatctcgcc ttcggtgacg agcgggtcgg ggattttgcc tgcgtcaaca aggcggccaa
      301 attcctccgg ccacgcttcg tacgggttgt gggggtcgaa aatgctcatc atggcgaaga
      361 acggccgtga gccatcctgc cgtttgaggt aaccgatcgt ccgctcggcg gcccagtgag
      421 tgaaatggag atgcgccggg tgatgcagga gtttgcggcc ttcccgtttc aactttttga
      481 agaaaaccgg atcgcgctcc ttgagccatt tcccgtagcc gttgagcggc gaatcgagat
      541 cgaggcaggc ctccagcgcc cactcgtaga catcgaatcc gtcgtgcgga tggcgctcgg
      601 agttctcgag catccggctg cggatgtgga gtttgccgaa cagcgcggtc tcgtagcccg
      661 cccgctggag gtgctcgggg aacaggacct cgtcctcagg caggttgccg tagacggtgt
      721 acacgccgtg cccgtgcagg tgcttaccgg tgaacatgct cgaccggctc ggggtgcaga
      781 tggggttggt cacgtagaat ttctcgaaca acgccccttc acgagccaga cggtcgagat
      841 tgggcgtgtg cgcagccgga aatccgtagc aaccgagcga gtcgtatcgc tgctggtccg
      901 tcatgaggag caggatgttc gggcgcgacg gggttggcat ggcaaactac cttttttcgc
      961 gttgatttcg ggaggtttcg caagcgaagg taccgacggc cgggccgcga aatcaacagc
     1021 gggaccggga atggtgtcgc ccgcgcttgc ccccgcccca tgagagcgct atcctgcaac
     1081 atgacggagg ggagtgaacg agcgaaccgg atgcgcggaa aggataccac atggacaatg
     1141 tgctgctgaa aacgagcgag ctttccgtcg tcgtcggaaa caacgaaccc ggttccggcg
     1201 acttcgccgc tcatcgcgcc ggatataacg gcatctggtc actgacgagc ctgcacagcc
     1261 ctgaaaactg cttcgtgccg ttctacgcgg ggctgaacct cgagcattgc atggacgacc
     1321 tgttcatgac cgaggagggg gttgacaact tcgagccgcg ccatgcaccg atggagctga
     1381 aacagctttc aggcggttct gcgtgcctct accagccgct cacaccgctt acgggcgtgg
     1441 aatccgagac cgtgttcgag gtaaaggaac cttacgtcat cgacatgcgc tttgccgcca
     1501 ccctgcaccg gccgccacgg gtggggagat ggttcggctt tttctgggca agttacgtca
     1561 atgcgcccga cagccctgcg ctgtatttcc gcgacgcgga cgggatgttg aacgcaatgg
     1621 ctccggacgt ccatggcgag ggtggagcca acacggtctg ccatgcgtcg gtcgaagacc
     1681 ctgccctcgg ggaccccggg cggaggtatg ccgggcacag ccttgctcat tcctggagtc
     1741 ggcggcggtt cggagcgccg ctcatgttcg gccggccggg cgacgggagg atgctgtacc
     1801 tccagatgtt cgatcaacag agcccggtca ggattacgat gtctccctcg ggtgggggca
     1861 ggaacgagga gaagaaggcc ttcaatccgg cctgggattt ccagtacgtg cggggcgaag
     1921 ccaccacggg gacggagatt cggcttcgca gccgaatcgt ctacaagcaa tatgacgggc
     1981 ttgaggagat cgaccggttc tacacggaat gggaacagca cgttgccggc tgactttcag
     2041 gcaagccacg aagcacgaat ttcagagcag cgacgcatca tgctcagatt catactgcct
     2101 tctgtgtgtg tccttctagt cgcgtatccc cgccctgcgg ctgcgcgagg ggagtgctgg
     2161 ggaacatgcc gccgacgagc cacgatctga gcaccacgtg gcctgcgttg tgcgccagaa
     2221 cggcggcggg taccgcactt tccgcggtcg gcgctgcggc gactcccgtc ccggcgaaac
     2281 agagaatggg tacgaacaga catgaaaaca ggcgcatggt ttggtgctcc ctgcagtggg
     2341 ggaattggaa gggatacccg taaatatgcg gcatctttct gcgtcagtca accccatccg
     2401 tcgagctggg aaggaacaat gttaccggcg acctagttgt agatgtgcga agtgtgggaa
     2461 atgttacacg aggattcgtg tctattacgg cttgacattt tcacgaggcc tccgtatcct
     2521 gctcgacggg cgtcatgaac gcagccacaa tacgggaagg aaggagcgcg catggggttg
     2581 ctgtctctgg tgattctact ggcaagcgcg tatgcgaccg cacggtacaa cgtgtttggt
     2641 gatgttgcct ggagcgaatg gccgaccctg gttctgaaca aaatccttgc gctgtcggcc
     2701 cttgcgcttc tggcaatcca caccatccgc cggcgcggcg ttcaagaaac atccgcgcgt
     2761 actatcgtcg gcgtcgccgg atttctggcg gccgttcacg ttttgctttc gctggtgctg
     2821 ctttcgccgg catactacgg caagcttttc aacctcgaca gactcactgc gtccgccgga
     2881 atcgcgatgc tgctgggcgc gctcgctgcg gcgacgctgg cggttggcgg aaaactgttg
     2941 gtcggaccgg atacgcgcga gcctcgcagg tacttcgctt tcatcagctt tgtgatcgga
     3001 ctgcatgccg cgttccaggg atttttcggg tggttcactc cctggaattg gccgggcggc
     3061 atgccgccta tcagccttct ttcatttgcg ctgggaattg tagccctcgt ggcagggctg
     3121 cgcaggggtg aatcgccgtc tcgcagtaac aggtaacggg aaccaggagg gccaaatggc
     3181 ggaaacaagg aacatcagaa aggtgctgcg ggcgaggccg acgctcgagg gtgcgggtgt
     3241 acatctgaat cgcgtgttcg ggggcgagca tgtgccgcag ttcgatccgt ttctgctgct
     3301 cgacgatttc agttcgaaca atccggcgga gtacatcaag ggcttccctt ggcacccgca
     3361 ccggggaatc gagacgatca cgtatgtgct tgcgggcgaa gtggaacacg gcgacagcat
     3421 gggcaactca ggtgtcattt cttcgggcga cgtgcaatgg atgaccgcgg gcggcggaat
     3481 cattcatcaa gagatgccga agggcgatgc acagggcgtg ctgaggggat tccagctctg
     3541 ggcgaacctg ccggcggcaa acaagatgat gccgccgcgg taccggggga ttcaggcgga
     3601 gcagattccg caggtggccc tgcccggcgg cgcaaccgcc cggatcatct gtggcgaagt
     3661 ggccggcgcg aaagggcccg tgggggacgt tgtcatcgag ccgctctaca tggatatcac
     3721 cgttccggcg ggcggcgagc tccactttcc ggtgcccaaa ggccatactg tgatggccta
     3781 cgtgattgac gggaaggggc agttcgcgcc ggagagcgat ccgttcgcat tcgacgtgga
     3841 aggccgcaac tacttcgatc tcgagcgcga atgccagata ggaacgagga accttgtggt
     3901 gtttggcgag ggggacgaac tcgcgatagc aggcggcgac gccggggtcc gcttcctcct
     3961 cattgcgggc aagccgattg gtgagccggt ggcgtggcac gggcctatcg tgatgaacac
     4021 gcaggccgaa ctgcgccagg ccatcagcga cctgcagaac gggacgttta tcaggtgact
     4081 cgcctctggt ttgccgacgt gcacgccaat ctcgcggcgt ttgaggccgt gctggcagac
     4141 gcgggtacgg cgg
//
LOCUS       DAOSOO010000245         4125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21716_length_4125_cov_5.806143, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000245 DAOSOO010000000
VERSION     DAOSOO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21716_length_4125_cov_5.806143"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLT86_13070"
     CDS             <1..809
                     /locus_tag="PLT86_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT37586.1"
                     /translation="MPVHILGGAADMDPILEIGKKHGVKIMEDSAQSAGATYKGRRVG
                     SMGDVNAYSLQFQKVITAGEGGVVTTNDEELFDRAVRAHDHGINRWNNVAEKNFCAEV
                     YRMSEMHGAFALAQLRKLESNLAAMRRHNRRIRKAVEELPGLTLRTLADVEGATGQSI
                     TFFLETADLAAKWREALEKEGIPNFVLYGGTMVFENPQVLHQRMPTKHACPFDCPLYE
                     GPITYAKEDYLQSREILDRSVWLYVSPLLTDDDCDDIVKAIRKVHTGLSA"
     gene            879..1964
                     /locus_tag="PLT86_13075"
     CDS             879..1964
                     /locus_tag="PLT86_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT37587.1"
                     /translation="MNVLLPENAYSVILAGGGGTRFWPQSRSQKPKQFLPFFGPRTLL
                     QHSFDLITPLTPPDRILVVTNSAQAGLVREQLPDVPEANVLSEPCARGTAAAVAWAAA
                     AIAGRNAKGTMTVVGADHWVRNPEALHAAVVRGHEVAERGHALVMFGLTPGGPNPHYG
                     YIIGAPEPEESELAATVRVLRFHEKPPAETARRYLSQKHCYWNSGMFSCSVETMHAEM
                     DLYAPNILRAVRAAIRSLPDHDAFREAYSGIPANSIDRAVLEHSANCYVVPTAIERVE
                     LRTWASLFSLLPRDTGGNVSIGDVRMLDCANTVQFGSGKLVAAIGMENCAIVANADAV
                     LVCALDRADDIGSLIYLAVEKGYEELR"
     gene            complement(1996..2967)
                     /locus_tag="PLT86_13080"
     CDS             complement(1996..2967)
                     /locus_tag="PLT86_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT37588.1"
                     /translation="MSHRIPVFKKAPRATIPWEHDPTTGRTPQARSKSVLTGIGIGLS
                     VLLVCLKGFAGWRSGSTAVISDAFNSVLDIISYSALYLSVRLQAKPADLTHHYGHHRA
                     EPLAGFVIAVFAAILGGMVLRDSVLRLLRPDPVRMSALSAIIVLFAIVSKVIIAIAYR
                     RGAKGGSPAMDAAAIDSRNDAMASSVALIGLIGGAVWPYLDGAAGLAIGAWILGSGVR
                     VGLENIGFLLGKAPSKAVLEELRAVAETVPGLIGTNNIRAHYVGNSMHVEVHIEVDAN
                     LSIREAHDLAKTVGQRLERLDDVYAAFVHTDPVDRGLKNPQQSEQAS"
     gene            complement(3042..4016)
                     /locus_tag="PLT86_13085"
     CDS             complement(3042..4016)
                     /locus_tag="PLT86_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT37589.1"
                     /translation="MERPRVYVHRVGSWAPRYLDDQNRSRLADFAEIADDASLDAPPP
                     DIAHRLEGVQAILSLNGAGAEDITEDALRAVGTVRIAVISHWFHGSHDRATAAWRAAG
                     VRVTDASWGNNFAVAQWTLGAMINGVFRVAELDRAMRAGEVWPDHHFTSSMLDGQRIG
                     LVGLGRIGRLVAGLLQPFHVEIVGYDKYVTAEEASKFNVRWCPLEQVMATSDIISLHL
                     PVTPETTRLITRAHIESIRKGALLVNSARTAILDYEAFRECLQKGLFRAIVDVFEPEP
                     PPVDDPLRTLPNVVMTPHIAGSTARMCHVCGRTAIEELRKQLVGQSSG"
BASE COUNT          869 a         1233 c         1175 g          848 t
ORIGIN      
        1 taatgccggt gcatattctg ggtggcgcgg ctgacatgga tcccattctg gagatcggaa
       61 agaagcatgg agtgaagatc atggaggatt cggctcagtc tgccggggca acctacaaag
      121 gtcgtcgcgt aggttccatg ggggatgtca atgcgtacag cctgcaattc cagaaggtaa
      181 tcaccgcagg tgaaggtggt gtggtaacga ccaacgacga agagttgttc gacagggcgg
      241 tacgggcaca cgaccacggg atcaatcgtt ggaacaacgt tgcggagaag aatttctgcg
      301 cggaagtgta ccgtatgagc gaaatgcacg gtgcgttcgc ccttgcccag ctccgaaaac
      361 tggagagcaa cctggcagcc atgcggcgcc acaacaggcg gattcggaaa gcagttgaag
      421 agctgcccgg cctcacactt agaactctgg ccgatgtcga aggcgctact ggacagagca
      481 tcacgttctt cctcgaaact gctgatctgg ccgcaaaatg gcgggaagcg cttgagaaag
      541 agggtatccc gaacttcgtg ttgtacggtg ggacgatggt tttcgagaat ccacaggtac
      601 ttcatcagcg catgccgaca aaacatgcat gcccgttcga ctgcccgctt tacgagggtc
      661 cgataaccta cgcgaaggaa gactacctgc aatcccggga gattctcgac cgctcagtat
      721 ggctttacgt ctctccattg ctcaccgacg atgactgcga cgacatcgtc aaagccatca
      781 ggaaagttca cacggggctg tctgcatgat ctgaagcacg ccaacgtgct tcagcgtgcg
      841 ttcaggcgca ctcatactct gcatggaagg ttgattcagt gaacgtgctg cttccggaaa
      901 atgcctattc cgtcattctt gctggcggag gaggaacacg tttctggcca cagagccgtt
      961 cacagaaacc caagcagttt cttccgttct ttggtcccag aacactcctg cagcacagct
     1021 tcgacctcat taccccgctc acacctccgg accggatcct ggtggtgacg aactcagctc
     1081 aagcggggct ggtgagagaa cagttgcccg atgttcccga agcgaacgtc ctttccgagc
     1141 cttgtgcgag gggcaccgcg gcagccgttg cctgggctgc cgcggcaatc gccgggcgga
     1201 acgcgaaagg aaccatgaca gtcgtgggcg cggatcactg ggtcaggaat cccgaggcct
     1261 tacacgcggc agttgtccgg ggacacgaag ttgccgagcg cggtcatgcg ctcgtcatgt
     1321 tcggactcac gccggggggg ccgaaccctc actacggata cataatcggc gctcccgaac
     1381 cggaggaaag cgaactggcg gcaaccgtga gggtgttacg cttccacgag aagccgcctg
     1441 cagagacagc tcggcgctac ctgtcccaga agcactgcta ctggaacagc ggcatgtttt
     1501 cctgcagcgt ggaaactatg cacgcggaaa tggatctcta cgcgcccaat attcttcggg
     1561 cagtgcgagc ggcaataagg tcccttcccg atcacgatgc gttcagagaa gcctattccg
     1621 gcattccggc aaacagcatc gaccgggcgg tactcgaaca ctccgccaat tgttatgtgg
     1681 tacccactgc gatcgaacgg gtcgaactcc ggacgtgggc gtcgctcttt tccttgctcc
     1741 cgcgcgatac gggcggcaac gtttcgattg gcgacgtcag aatgctggat tgcgcgaaca
     1801 ccgtccagtt tggttcgggc aaacttgttg ctgcgatcgg gatggagaac tgcgccatcg
     1861 tggcgaacgc tgacgcagtt ctggtgtgtg cccttgaccg cgccgacgat atcggttccc
     1921 tgatctatct tgcggtcgag aaaggatacg aagagctgcg ataacccagt accgggttcc
     1981 gactgcgttc aggtgctaag acgcctgttc gctttgctgc ggattcttca gcccgcgatc
     2041 cacggggtcc gtatggacaa acgccgcgta cacgtcgtcc agacgttcca atcgctgacc
     2101 taccgttttc gcgagatcgt gggcctctcg tatgctgagg tttgcgtcaa cctcgatgtg
     2161 cacctcgacg tgcatagagt tgcccacgta atgcgcccgg atgttgttgg ttccgattaa
     2221 tcccggcacg gtttctgcca ccgcgcgcaa ctcctccaga accgctttcg atggtgcctt
     2281 tccaagcaga aaccctatgt tctcaagccc cacccggacg ccgctgccca gaatccatgc
     2341 gccgatcgca aggcccgccg ctccatcgag gtaaggccat accgcgcctc cgataaggcc
     2401 gatcagtgcc accgaagacg ccattgcgtc gttccgggag tctattgccg ccgcatccat
     2461 cgcggggctc cctcccttcg cgccgcgtcg gtacgcgatg gcaataatca ctttgctgac
     2521 gatggcaaac agaacgataa tcgcgcttaa ggcgctcatt cgaaccggat ccggcctcaa
     2581 aaggcgtagg acggagtccc ggagcaccat tcccccaagt attgccgcaa ataccgcgat
     2641 aacgaagccg gcaagaggtt ctgcccggtg atggccataa tggtgcgtca ggtccgccgg
     2701 tttcgcctgc aaacggacgc tgaggtacag ggcgctgtac gaaatgatgt cgaggaccga
     2761 gttgaatgca tccgagatca ccgcggtgct tccggatcgc catcctgcaa accctttgag
     2821 gcacaccagc agtacactga gaccgattcc aattccggtc agaacactct tgctccgtgc
     2881 ctgtggcgtt cgaccagttg ttggatcgtg ctcccagggt atcgtggcac gcggtgcctt
     2941 tttgaacacc ggaattcggt gcgacatcag caaacaccct tctccagacg tcgttcaggt
     3001 acgcctgatg gtcagctcac ggcaacgtga tcaggttgcc atcaccctga agactggccc
     3061 acgagttgct tccgcaactc ctcaatggcc gttcgcccgc acacatggca catccgagcc
     3121 gttgaacctg cgatgtgcgg ggtcattacc acgttcggca gggtgcgcaa tgggtcgtca
     3181 acagggggcg gttccggttc aaacacgtcc acgatagcgc ggaacaggcc cttctgcaga
     3241 cactcacgga acgcttcgta atccagaatc gcagttctcg ccgagttgac cagaagcgcg
     3301 cccttccgaa tggattcaat gtgggctctt gttatcagtc gcgtcgtttc cggcgtaacc
     3361 ggcagatgca ggcttatgat gtcggaggtt gccattacct gttccagagg acaccagcgg
     3421 acgttgaact tcgatgcctc ctccgcggtg acgtatttgt cataacccac gatttccaca
     3481 tgaaacggct gcaacaatcc cgcgacgagc ctgccaatgc ggcccagacc cacaaggccg
     3541 attctctgac catccagcat gctgctcgtg aaatgatggt cgggccagac ttccccggca
     3601 cgcatggcgc ggtcaagctc tgcaacgcgg aagacaccgt tgatcatggc gccaagggtc
     3661 cattgcgcta cggcaaagtt atttccccat gatgcatcgg taactcgcac ccctgcggct
     3721 ctccacgcag ccgttgcccg atcgtgtgac ccgtggaacc agtgggaaat caccgcgatc
     3781 cgaaccgttc cgaccgcccg cagggcgtct tcggtgatgt cctctgcgcc cgcgccgtta
     3841 agcgacagga tcgcctgcac cccttcaagg cgatgtgcga tgtcgggagg cggtgcgtct
     3901 agggaggcat catccgctat ctccgcgaaa tctgccaatc tggaacgatt ctggtcatcc
     3961 agataacggg gagcccacga tccgacgcgg tgcacgtaca ctctgggtct ttccatttct
     4021 ctgcactcct tttcggcgtt tcagttttcc ggctgcgcaa aacgtatgtc aacgcacacg
     4081 gggaaatggt ccgacgggta tttgccgtct cgttcaaacg cgacc
//
LOCUS       DAOSOO010000246         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21739_length_4122_cov_6.052127, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000246 DAOSOO010000000
VERSION     DAOSOO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21739_length_4122_cov_6.052127"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLT86_13090"
     CDS             <1..741
                     /locus_tag="PLT86_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOT37590.1"
                     /translation="GSTEANELADFCERRFGWTIRRDSGLGLKPISRTGTERLVRAAI
                     DYAIKNGRKSVTLVHKGNIQKFTEGAFRSWGYELAKREYRDHVVTWEECGGDVPAGKV
                     LIKDAIADIFLQQVLTRPVEFDVIATTNLNGDYISDALAAQVGGIGIAPGANINFETG
                     HAIFEATHGTAPKYAGQDRVNPGSVILSGEMMLRYIGWGEAANYVVKGLERTIAQRTV
                     TYDFARLMENAREVRCSEFSSAIIANMS"
     gene            818..1681
                     /locus_tag="PLT86_13095"
     CDS             818..1681
                     /locus_tag="PLT86_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT37591.1"
                     /translation="MATTHEQLEANSLGADNSLRRYLEDIRTIPMLTREEEVELARRA
                     RAGDDAARARIVRANLRFVVSFAKRYQHRGLPLVDLVAEGNLGLVRAVDRFDETLGYK
                     FISYAVWWIRQAILQALSRQARPMRIPAGRTNRMLQLERLHDRLEQETSDSVAWDRVV
                     DEVSPGAPADGVLSFQAKVSSLDAHVGEDDVHLSEILFDERAASPAEELEMQDCREAL
                     QRAMEGLDPREQMIVKRFFGLDTEERRTLEEIGVEFRLTRERIRQIKDRALRKLRHAS
                     RNQTLRDYLVG"
     gene            complement(1754..>4122)
                     /locus_tag="PLT86_13100"
     CDS             complement(1754..>4122)
                     /locus_tag="PLT86_13100"
                     /inference="COORDINATES: protein motif:HMM:NF027717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5010 domain-containing protein"
                     /protein_id="HOT37592.1"
                     /translation="NGVLSFSVAPAHLDGVAHGVFVIVDADSGSGDLAVSYRAQTNSA
                     FVSTSAVEVRTQGAVTKRVFWLDTLDVPGGVSVVFSGFGAGGITLRTVRVWGAPGPYV
                     SPAHQDFAGRSFSPRDPLVLTYYFYWYDVYGGSHMRNGDGSDALQDHPQATDDFSFKS
                     VDWHVSQMRDMMEAGIDVVLPVYWGGSVEMTGWAVEGIEKLVIAQQQMRARGENPPQI
                     GMFFDTSTLEYTEHVLKFGAMKSDLTTPFGKAFFFKQIRDFFSLIPPEMWARVNGQPL
                     VVLYGAGFASRHDQSTITTMYDLFGTSFGGLRPFVIKEVSWQVYTPFEYAWGAALNGP
                     NGYTGTAAVGPGYNDSAVPGRTTPVRDRENGEFYKRSWRQALSWGRKFVLVETWNEFH
                     EGTDIARSREYGDYYIRLTRQFADIHKGPVRFSDPQPSGWVRTLSTNVSMRAEVDNVA
                     RLDPLSVECRYSTDSGATWMTVPARAVAQGDSLYLLTAENVPFGQASTALATPNRFMF
                     VIRSAVGDTAFESLPQPVWLGYPPSAEGSVVLGAQNNEQGLHHILMNAGDGWTAPGSR
                     GDRGARCNLPQSGQQGSYFYFQVDDSLICNGSDPEAWVEVTYWDGEGTWFELQYDSRG
                     TAFLDAYKAAGRRSITGVQEWRTTTFNLKDAWFGNRQNGGSDFRVYANGTAWIHQVRV
                     YTKAHPAGVTSGPPKGQPLVWMGDPQPNPFNSSVLVPLRIPDGRTAVVSVVNAAGQVI
                     RQWNESAGSRLLAWDGTDKAGRPLGSGVYFVRVQCGETALTRRVTLLR"
BASE COUNT          891 a         1233 c         1201 g          797 t
ORIGIN      
        1 ggctccactg aggccaacga actggccgat ttctgtgagc ggcggttcgg atggaccatc
       61 cggcgggatt cggggctggg gctgaagcca atcagcagaa ccggaacgga acgcctcgta
      121 cgcgcggcaa ttgactatgc catcaaaaac gggcggaaaa gcgtcactct tgtccataag
      181 ggcaacattc agaagttcac cgagggcgcg tttcgttcgt ggggatacga actggcgaag
      241 agggaatacc gggaccacgt ggttacgtgg gaggaatgcg gtggggatgt tcctgcgggc
      301 aaggttctca tcaaagacgc catcgcggac atctttctcc agcaggttct gacgaggccg
      361 gtggaatttg acgtcattgc aaccacaaac ctcaacggtg actacatttc cgacgctctc
      421 gccgcgcagg ttggcggaat cggcatcgcg ccgggggcga atatcaattt tgagaccggt
      481 catgcgattt tcgaggccac gcatggaact gctccgaaat acgcggggca ggacagggtt
      541 aatccgggat cagtcattct ttcgggcgag atgatgcttc ggtacatcgg gtggggagaa
      601 gcggcgaatt acgtagtgaa gggcctggag cgaacgattg cgcagcggac agtcacttat
      661 gatttcgcac ggttgatgga aaacgcacgt gaagtgcggt gctcagaatt cagttcggcg
      721 ataatcgcca acatgtcata gagcgcgtcg catcactggt acccgtttcg tgcggcgaac
      781 gtggacgcga tgcgctcttt cgagcggaag ggtttccatg gcaacgacgc acgaacaact
      841 tgaggcaaac agtcttggtg cggacaacag ccttcggcgg tatctcgaag acatccgcac
      901 cattccgatg ttgacgcgcg aggaagaggt tgagctggcg aggagggcgc gcgcgggcga
      961 cgacgcagca agggcacgga tcgtgcgcgc aaacctccgg ttcgttgtca gtttcgccaa
     1021 acggtaccag cacagggggc tgccgctggt agatcttgtt gcggaaggga accttggtct
     1081 ggttcgcgca gtcgaccgtt ttgacgaaac gctggggtac aagttcatat cgtacgcggt
     1141 gtggtggata cgtcaggcga tcctgcaggc gctttcacgg caagcgcggc ccatgcgtat
     1201 ccctgccggc cgcacaaacc gtatgctcca actggagcgg ctccatgaca ggctcgaaca
     1261 ggaaaccagc gactcggttg cctgggatcg ggtagtggac gaggtctccc cgggagcgcc
     1321 ggcggacgga gtgctctcct tccaggccaa agtcagctca ctcgacgccc acgtaggcga
     1381 agatgacgtg catctctccg aaatcctttt cgatgagcgc gctgcttcgc cggccgagga
     1441 actggaaatg caggattgcc gggaagcgct tcaacgggcc atggaagggc ttgacccgcg
     1501 cgaacagatg atcgtgaagc gattcttcgg gctcgatacg gaggaacgca ggacccttga
     1561 agaaatcggc gtggaattcc ggttgacgag agaaagaatc cggcagataa aggacagagc
     1621 tctgagaaaa ctgcggcacg cctcccgcaa tcaaacgctc agagattacc ttgtcggata
     1681 gctcccacac ctgtggagaa cgagggcgcg gacatcctgg gccaggggaa tgcccactgc
     1741 gttcggtttc gcgttatcga aggagggtga ctctccgcgt aagcgctgtt tctccgcatt
     1801 gcacccgtac gaaatacacc ccgctcccga gcggccggcc ggccttatca gtcccatccc
     1861 aggccagcaa tctggaaccg gcactctcat tccactggcg gatcacctga cccgcagcgt
     1921 tgacgacgct gacaactgcc gtccgcccat ccggaatccg cagaggcaca agcacagagg
     1981 aattgaacgg attcggttga ggatctccca tccatacgag gggttgtccc ttcggaggcc
     2041 ctgaagtgac tcccgcagga tgagcttttg tgtacacccg cacctggtgg atccacgccg
     2101 tcccgtttgc gtacacccgg aaatcggatc ctccgttctg tctgttcccg aaccaggcgt
     2161 ccttgaggtt gaaggtggtg gttcgccact cctgaacgcc ggtgatcgac ctccttcccg
     2221 ccgccttgta ggcatcgaga aatgcggtgc cacgggaatc gtactgaagc tcaaaccagg
     2281 taccctctcc atcccagtag gtgacctcca cccacgcttc ggggtcgctc ccgttgcaga
     2341 tgagggagtc atccacctga aagtagaagt agctcccctg ttgcccagat tgcggcagat
     2401 tgcaccgcgc accgcgatcg cctcttgatc ccggagcagt ccacccgtcg ccggcattca
     2461 tcaggatgtg gtggagaccc tgttcgttat tctgcgcacc aagaacaacg cttccttctg
     2521 cagaaggagg gtaccccagc cagactggct gcggaagact ctcgaacgcc gtgtccccca
     2581 ccgcgctcct gattacgaac atgaaccggt tcggcgttgc aagcgcggtg cttgcctgtc
     2641 caaaaggcac attctccgca gtcaacaggt agagcgagtc accctgggcc accgcgcgcg
     2701 ctggaaccgt catccacgtc gctccgctgt ctgtcgagta acggcactca acagacagag
     2761 ggtccagccg ggcaacgttg tcaacctccg ctcgcatcga cacgttcgtg gacaacgttc
     2821 gaacccatcc cgacggttgg gggtcggaaa aacgtaccgg gcccttgtga atgtccgcga
     2881 actggcgggt caaccggatg tagtaatccc cgtattcgcg gctcctcgca atgtcggtac
     2941 cctcgtggaa ttcgttccag gtttccacca ggacgaactt tcttccccag gaaagcgcct
     3001 ggcgccacga gcgcttgtaa aactccccat tctcacggtc ccgaacagga gtcgtccgtc
     3061 ccggcaccgc ggaatcgttg tatcccggac cgaccgcagc ggtccccgtg tacccgtttg
     3121 gaccgttcag ggcggcgccc catgcgtatt caaacggcgt gtagacctgc cagctcactt
     3181 cctttatcac aaaaggacgc aatcctccga aactggtccc aaacaaatca tacatcgtcg
     3241 taattgtgct ctggtcgtgc ctggacgcga atccggcgcc gtagaggacc acgaggggct
     3301 gtccgttcac tcgagcccac atctcgggag ggataagtga gaaaaagtcc cggatctgtt
     3361 tgaaaaagaa cgctttacca aacggcgtcg taaggtcgct tttcatggcg ccgaatttca
     3421 gcacgtgctc cgtgtattcc agcgtcgaag tatcgaaaaa catcccgatc tgcggcggat
     3481 tttccccgcg tgctcgcatc tgttgctggg caatcacgag cttttcgatt ccttccaccg
     3541 cccagccggt catttcgacc gacccgcccc agtaaaccgg taacacgacg tcaatgcccg
     3601 cttccatcat gtcgcgcatc tgcgatacgt gccagtcgac ggacttgaaa ctgaaatcat
     3661 cagttgcctg agggtggtcc tgcagcgcgt cggaaccatc tccgtttcgc atgtgggagc
     3721 caccatacac gtcataccag tagaagtagt acgtcaacac gagtgggtcg cgtggcgaga
     3781 acgaacgccc tgcgaaatcc tgatgagcgg gagaaacgta gggacccggc gcgccccata
     3841 cgcgaacggt gcgcagcgta ataccacctg ccccgaatcc gctgaagacg acggagacac
     3901 ctcccggcac atccagcgtg tccaaccaga atacccgctt tgttactgct ccctgggtgc
     3961 gtacttccac tgcagatgtc gaaacgaacg cgctattggt ctgcgccctg taactgacag
     4021 ccaggtcccc actcccgcta tccgcgtcaa caatgacgaa cacaccgtgt gctacaccat
     4081 cgaggtgggc cggggcaacc gaaaacgaca gcacgccgtt gc
//
LOCUS       DAOSOO010000247         4112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21811_length_4112_cov_5.129406, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000247 DAOSOO010000000
VERSION     DAOSOO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21811_length_4112_cov_5.129406"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLT86_13105"
     CDS             <1..653
                     /locus_tag="PLT86_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37593.1"
                     /translation="WTAQLYRHYGIEGNTDLLSKRYGFTYELPHIKNPDFAFNWEGWD
                     VPVNIERNCTVKSIDGYSALQGRWSVTDVGNSVMVVKRSSVRPNAISQPIRHLVPGRL
                     YSVKLISGDYDDFVNGKSEEKLHMLKIGIEGGELVKEKCFQSPLPNHPTTKLEPFGRD
                     HRYWFNLHHLVFRATAKTGKITISDWATPREPGAPVGQSTMYNFVELQPYWTGDEQ"
     gene            688..1719
                     /locus_tag="PLT86_13110"
     CDS             688..1719
                     /locus_tag="PLT86_13110"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37594.1"
                     /translation="MEHVIVFKQPGRFGGWPANNGIWSWGDEIVTGFNEGALDLHKFP
                     GHPIKGDENSGRRQVRSRDGGVTWELIRKPHFDIHEMPLVNCPGGITFTQKDFAMKVL
                     ATGVHAGSVTYFYVSYDRCTTWEGPYQMPFFGLKGIAGRTDYQVMGDHDAIVFLAANK
                     SDGYEGRAFCARTRDGGKTFQFLSWIGPELAGWTIMPASVRLPNGRLLAALRCNFRPE
                     HGCQVFSSDDEGCTWSYLSEPVKPDMGGNPPAMIRMTDGRLVLTWGHRVQPRGIRAFV
                     SEDNGKTWTDPIILREDGGASDVGYTRTVQRADGKIVTVYYFNDPPTEEGVNTERYIA
                     ATIWHPDEF"
     gene            complement(1789..2442)
                     /locus_tag="PLT86_13115"
     CDS             complement(1789..2442)
                     /locus_tag="PLT86_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT37595.1"
                     /translation="MNPQKLLVFGGHPDDPESSCGGLIANAVAAGHTAHCLYGTNHRV
                     GYTYFGRPEKEVRSAEAVAACKILGATWEFLDYPEHLEVNPETRRRVADVIAAYAPHI
                     VVAHWPVDTHPDHRSVGILAFEAYLLHRSYAFYFFEVMTGRQSIRFTPSHYVDISAVA
                     ETKRAAVMCHLSQTPDAIWVDHEIMHRFRGRECDVERAEAYLRVDREPGGLLPGMVF"
     gene            complement(2473..3825)
                     /locus_tag="PLT86_13120"
     CDS             complement(2473..3825)
                     /locus_tag="PLT86_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37596.1"
                     /translation="MPRSVGVSSYALVGVVITIVCIGCAAITGRGAQRTEASVNSEEQ
                     QIRLALEQWKQAPEQDTRGMAPRTSFNFARAAVQVTGSAAVVEPVYALTGWGLGVFSF
                     SLRKKDDQWVFKSWRHVGEGVIETALPDLDPGYLPLLNRWMEANLGWVQKTLRENPPN
                     GGDQILRRHALCMLDEPLHIRSAPALEPVKQFIRSNVDLAISQIRTERVAAGATIWKF
                     YNHGWVVRTANHTWAHDIYEGPEGLITGDQIDAILDKVEVLFLSHWHGDHSSPRVMKR
                     ARDKGIPVLVTPWPAEGGYADYIRSEMTDANGSLDWLTVVQPGSSGEIRGLQYHAFPG
                     HQDQLLNNAYAVTVDGMTILQMGDQWNLDDFVWIDSVKTRQRTDILLPNVWTAQMKRV
                     LAGVAPRVVVPGHENEIGHSFEHREPYDQAYEVLAPAECQWHVMAWGERLHVEPAPQQ
                     "
     gene            complement(3853..>4112)
                     /locus_tag="PLT86_13125"
     CDS             complement(3853..>4112)
                     /locus_tag="PLT86_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37597.1"
                     /translation="GYQFHRPETGDGFAMMFRRPKSEEGTKTVCLRAMERDLWYHVRI
                     EGSSNCQHFIGWQLEDLSVNITECPGTSLIRYAPAESESEG"
BASE COUNT          835 a         1222 c         1223 g          832 t
ORIGIN      
        1 aatggaccgc gcagctgtac cgccactacg gcatcgaggg aaacaccgac cttctcagca
       61 aacgttacgg gttcacgtat gaacttccgc acatcaagaa cccggacttc gcgttcaact
      121 gggaaggttg ggacgttccc gtcaacatcg agaggaactg caccgtgaag agcatcgacg
      181 gttacagtgc tctgcaggga cgatggtcgg ttacggatgt gggcaattct gtcatggtgg
      241 tgaaacggag ttcggttcgc ccgaatgcaa tttcacagcc gatccggcat ctcgttccag
      301 gtaggctcta ttccgtgaaa cttatcagcg gagactacga cgacttcgtg aatgggaagt
      361 cggaagagaa gctccacatg ctgaagatcg ggatcgaagg cggtgagctg gtgaaagaga
      421 agtgtttcca gtcgccgctg ccgaatcatc caacgacaaa actcgaacca ttcgggcggg
      481 atcaccgcta ctggttcaat ctccatcatc ttgtcttccg tgcaacggca aaaaccggga
      541 aaatcaccat ttctgactgg gcgactccgc gcgaacccgg agcacctgtc ggccagagca
      601 ccatgtacaa tttcgtcgaa ctccagccgt actggaccgg cgacgagcaa taaaccgggg
      661 aaacacttac cgcaaaggat tcggaacgtg gaacatgtga tcgttttcaa gcagccggga
      721 cgttttggcg gatggccggc aaacaacggt atctggtcgt ggggtgacga aattgtcacg
      781 ggattcaacg agggcgcgct ggacctgcac aaattccccg ggcacccgat caagggagac
      841 gagaactccg gtagaagaca ggtccgaagc cgtgacggcg gcgttacatg ggaactgatc
      901 cggaagccgc actttgacat tcacgagatg ccgctcgtca actgcccggg cgggataacc
      961 ttcacgcaga aagacttcgc gatgaaggtg ctggccaccg gggtgcacgc gggatcagtc
     1021 acatatttct acgtgtccta cgaccgatgc acgacgtggg agggcccgta ccagatgccc
     1081 ttcttcgggc tgaaggggat cgccgggcgc acggattacc aggtcatggg cgaccacgac
     1141 gcgatagtgt tcctcgcggc gaacaaatcc gatggatacg agggaagggc gttctgcgca
     1201 cgaaccagag acggtgggaa aactttccag tttctttcct ggatcggtcc ggagctggcc
     1261 gggtggacca tcatgccggc ttcggtacgc ctcccgaacg gccgccttct cgccgcgctt
     1321 cgttgcaact tccgcccgga acatggatgc caggtgttca gttccgatga cgaaggctgc
     1381 acgtggagtt acctgagcga gccggtgaag cccgacatgg gaggcaatcc acccgcgatg
     1441 atcaggatga cggacggacg tctggtgctt acgtggggac accgcgttca gccgcgcggc
     1501 atccgcgcat tcgtgagcga ggacaatggg aagacctgga ccgacccgat catcctgcgc
     1561 gaagacggtg gcgcgtcaga cgtcggctac acgcgcaccg tgcagcgcgc ggacggaaaa
     1621 atcgtgacgg tgtactactt caacgatccg cccacggaag aaggcgtgaa taccgagagg
     1681 tatatcgcgg caacgatctg gcacccggac gagttctagc gttcgatctt gcactgcggc
     1741 gggtcctgcc cgccgcagtg cgttttcgcg aaaacgcgct cccgcctttc agaacaccat
     1801 ccccggcaac aatcccccag gttcccgatc cacacgcaga taggcttccg cacgctctac
     1861 gtcgcactcc cttcctcgga agcggtgcat gatttcgtga tccacccaga tcgcgtccgg
     1921 tgtctggctg agatggcaca tcaccgcggc gcgtttggtt tcggcgacgg cggaaatgtc
     1981 cacgtagtgg ctgggcgtga aacggatgga ctgcctgccc gtcatcacct cgaaaaagta
     2041 gaacgcgtag gatcggtgga ggaggtaggc ttcgaaggcg aggatgccga ctgagcggtg
     2101 gtcggggtgg gtgtccaccg gccagtgggc gacgacgatg tggggtgcgt aggccgcgat
     2161 cacatcggca acgcgccgac gcgtttcggg gttcacttcg agatgctccg ggtagtcgag
     2221 gaactcccac gtcgcgccga ggattttgca tgcggcgacg gcctctgcgg agcggacctc
     2281 cttttccggt ctgccgaagt acgtataacc gaccctgtgg ttggtgccgt agaggcagtg
     2341 tgcagtgtgc ccggcggcga ccgcgttggc gatcaggcct ccgcaactcg attcggggtc
     2401 gtccggatgc cctccgaaga caaggagttt ctgtgggttc atgcgtttgg ttccagtaga
     2461 aaagggggcg cgctattgct gcggcgcagg ctccacgtgc aggcgctcgc cccacgccat
     2521 cacatgccac tggcattccg ccggtgcaag gacctcatac gcctgatcat acggttcgcg
     2581 gtgctcaaat gagtggccga tttcgttttc gtgacccggt acgacaacac gaggtgcaac
     2641 gccggccaga acccgcttca tctgtgccgt ccagacattc ggcagcagga tatccgttcg
     2701 ctggcgcgtt ttcacgctgt caatccagac gaagtcgtcg agattccact ggtcacccat
     2761 ttgcagaatc gtcatgccgt ccacggtgac ggcatatgcg ttgttgagga gttgatcctg
     2821 atgccccggg aacgcgtggt actgcaatcc ccgaatttcc cctgaagaac ccggttgcac
     2881 aaccgtgagc caatcgaggg agccgttggc gtcggtcatt tccgacctga tgtaatccgc
     2941 atatcccccc tctgcgggcc atggtgtgac gagtaccgga atccccttgt cccgtgcacg
     3001 tttcatcacc cgcggcgatg agtggtcgcc atgccagtgg ctcagaaaca gcacctcgac
     3061 cttgtcgagg atcgcatcta tctggtcgcc cgtgatcagc ccttcagggc cttcgtagat
     3121 atcgtgggcc caggtgtggt tcgcagttct taccacccag ccgtggttgt agaatttcca
     3181 gatggtggcg ccggcggcaa cccgctcggt gcggatctgc gaaattgcca agtcaacatt
     3241 cgatcggatg aactgtttca ccggctcgag tgcgggcgca ctgcggatat gcaacggctc
     3301 atccagcatg cacagtgcgt ggcgccgcag tatctggtcg ccaccgttgg gcgggttttc
     3361 ccgcaacgtc ttctgaaccc aaccgagatt tgcctccatc catcggttca ggaggggaag
     3421 gtacccgggg tcgagatcgg ggagcgcggt ctcaatcacg ccttcaccga catggcgcca
     3481 gcttttgaaa acccactggt catccttttt gcgaagagag aagctgaaca ccccgagtcc
     3541 ccaccccgtg agggcgtaca caggttcgac caccgccgcc gaacccgtaa cttgaacggc
     3601 tgcccgggcg aagttgaaac tcgttcgcgg agccatgccg cgcgtgtctt gttccggtgc
     3661 ctgtttccat tgttccaatg caaggcgaat ctgctgttct tcgctgttca ccgatgcctc
     3721 cgttcgttgt gcgccgcggc cggtgatggc cgcacatccg atacacacga tcgttattac
     3781 cacgcccacc aacgcgtagc tcgacactcc taccgacctt ggcatcatct tcttcccatt
     3841 gacgcggtcc gttcaaccct ctgattcact ttccgcggga gcgtacctga tcaggctggt
     3901 tccggggcat tcagtaatgt tcacagaaag gtcctccagt tgccaaccta tgaaatgctg
     3961 gcagttcgaa ctgccctcaa tccgcacgtg ataccagaga tcgcgttcca tggccctcag
     4021 acacacagtc tttgtcccct cttcgctctt cgggcgtcgg aacatcatcg cgaatccgtc
     4081 cccggtttcc ggtcggtgga actggtagcc ga
//
LOCUS       DAOSOO010000248         4107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21842_length_4107_cov_4.324038, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000248 DAOSOO010000000
VERSION     DAOSOO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21842_length_4107_cov_4.324038"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLT86_13130"
     CDS             <1..829
                     /locus_tag="PLT86_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT37598.1"
                     /translation="GVLKQANPRSLLALLQAGPGALESAQRAAEKSLRAGDAVRPLKD
                     LTLDAPVPRPGKLLALAGNYGEHLKESGHEWAEKAVTTPRLFIKPTTTVTGPRSPIIY
                     PKNGNHIDWEGELGVVIGKTARFVKAKNGMDHIAGFLVINDVSERAVKIGSGRKERDG
                     DKWFDWILGKWFDTFAPMGPAFVTKDEIADVHALHLETRVNGVVKQNASTGQMIFSVA
                     ELVEWASRWMTLEPGDIISTGTPSGVGSASGTFLKPGDVVEVEIETLGVIRNRVVKG"
     gene            862..1344
                     /gene="tsaA"
                     /locus_tag="PLT86_13135"
     CDS             862..1344
                     /gene="tsaA"
                     /locus_tag="PLT86_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOT37599.1"
                     /translation="MERVSLPVIGVIHSPFEKLEGMPIQSAGARDVEGTVEVHPEYAE
                     GLRDLEGFSHVYLIYWFHRAQRTELTVVPFLDTEKRGVFATRSPLRPAHIGLSIVELL
                     RVEGPTLTVRGVDILDGTPLLDIKPYVPEFDHRPNATSGWLKAAKGNPLEARADGRFV
                     "
     gene            complement(1503..2561)
                     /locus_tag="PLT86_13140"
     CDS             complement(1503..2561)
                     /locus_tag="PLT86_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37600.1"
                     /translation="MKYKPDWPDAKARLTALWHHELLDRPCIAVTAPSGRSFPHPPAP
                     SAPRDRWLDPGWVLQSMEAQLENTWWGGEAVPSCLMMAGWVLCLGGTPRFAQDTIWFD
                     TKDVDFSLPPPFRYEPDNPWVAEYRALLDAALDFAGRDDFLVGKPLLLPANDLASTLM
                     GTETFLLALVDHADWMREAIVTGAKEQLRLRMELGDHVRSRHEFWYGNAGWMQFWAPE
                     PYYGTQSDVSCMLSPAMFDRFIVPELEVYGEAFGAIWYHLDGGDARQHLPRLLSLPYL
                     KVVQYTPAPGEAPNGPGHLDLYREIQAAGRIVHIEVEKSAVEPLVKALDPSLLMLHTH
                     CGSIAEGEALLEAAKHWV"
     gene            complement(2601..3137)
                     /locus_tag="PLT86_13145"
     CDS             complement(2601..3137)
                     /locus_tag="PLT86_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOT37601.1"
                     /translation="MSIPRPAPPVLLIAAVTWSPEVAEAGIVARLEDVWGPISTMGPA
                     FCFDDYTEYYAEEMGAGLTKRILAFAEPVRPEHLADIKLRSNELERFWHTAEGARRVN
                     IDPGYLCALTLVLASGKPSAYRIYLRDGIWAEITLRYERGSYAPLSTTYPDFRAAETI
                     AFLNSLRPRALEVGRETR"
     gene            complement(3140..>4107)
                     /locus_tag="PLT86_13150"
     CDS             complement(3140..>4107)
                     /locus_tag="PLT86_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT37602.1"
                     /translation="VNRDGKPDIIMGNANGETWQWLSLPGEPAWVFDLPRRVLSEGRP
                     FRIVPGAWGSIQGPGEARWGYVAPEITDWNGDGLLDLILADALGKHRFYPALENGADG
                     LVLGAEDAVRFADPASTIKPAWTWWDPQNGELVTQWRTRTEVLDWNGDGVTDYVSLDH
                     EGYLALFPGIVTPQGKRIGALQRVFLNEQGEPLLLNPMRNGHSGRYRLAFADWDGDGD
                     LDLIRGVAEPTHNRMHTVETEAGNAFLYENIGGDRFAFRGEMVPDPGLRLAAHAACPQ
                     PVDWDGDGRLDMLIGGEDGLIYAFHRAYLEQDLPKVRVQTVRVRR"
BASE COUNT          708 a         1261 c         1431 g          707 t
ORIGIN      
        1 cggggtcctg aaacaggcga atccgcgctc cctgctggcg ctgttgcagg ccgggccggg
       61 cgcgctggaa tcggcgcaac gcgcggcgga aaaaagcctc agggccgggg atgcggtgcg
      121 cccgctgaag gacctcaccc ttgacgcgcc ggtgccgagg ccgggcaagc tgctggccct
      181 ggcgggcaac tacggtgagc acctgaagga atccggccac gaatgggcgg agaaggcggt
      241 caccacgccg cgcctgttca tcaaacccac caccaccgta accggccccc gcagcccgat
      301 catctacccg aagaacggca atcacattga ctgggagggc gagctgggcg tggtgatcgg
      361 caaaacggcg cggtttgtga aagcgaagaa cgggatggat cacattgccg ggtttctcgt
      421 catcaacgac gtctccgagc gcgcggtgaa gatcggctcc ggccgcaagg agcgggacgg
      481 cgacaagtgg ttcgactgga ttctggggaa gtggttcgat acgtttgcgc cgatgggccc
      541 ggcgttcgtc acgaaggacg agatcgccga tgtccacgcg ctgcatctgg agacgcgggt
      601 gaacggcgtg gtgaagcaga acgccagcac gggccagatg atcttcagcg tcgccgagct
      661 ggtggaatgg gcgtcgcgct ggatgacgct ggagccgggt gacatcatct ccacgggcac
      721 gccgtcgggt gtcgggtcgg cgtccggcac gttcctcaag ccgggcgatg tggtggaggt
      781 ggagatcgaa acgctgggcg tgatccggaa cagggtggtg aaggggtaga aagccgttcc
      841 gccgggacgc aggaggacgc gatggagcgg gtaagtctgc cggtgatcgg ggtgattcac
      901 tcgcccttcg agaagctcga agggatgccg atccagtcgg cgggcgcgcg tgatgttgaa
      961 gggacggtag aggtccaccc cgagtatgcg gaaggcttgc gggacctgga ggggttcagc
     1021 cacgtctacc tgatctactg gttccatcgg gcgcaacgca ccgaactgac cgtcgtgccg
     1081 tttctggata ccgagaaacg tggcgtgttc gcgacgcggt cgcccttgcg gccggcgcac
     1141 attggccttt ccatcgtgga gttgttgcgg gtggaggggc cgacgctgac ggtgcgcggc
     1201 gtggatatcc tcgatggaac tccgttgctg gacatcaaac cctacgtgcc cgagttcgac
     1261 caccgtccga atgcgacctc cggctggttg aaggccgcaa aggggaaccc gttggaggcc
     1321 agggcggacg gacgattcgt gtgagggggt gtccaggaaa ggcggaatga cggcgaaagt
     1381 gcttcggtag cacgggcgtc tcgcccgtgc cgggtgcctg ccaccgcgaa gactgcacgg
     1441 gcgggacgcc cgtgctacgc aaagcttgtg gacgacatcg ccagaggcgg cggagagcgc
     1501 cgtcacaccc agtgtttcgc tgcttccagc aatgcttccc cctccgcgat gctgccgcag
     1561 tgcgtgtgca gcatgaggag ggagggatcg agcgccttca ccagaggttc cacggcgctc
     1621 ttctccacct caatgtggac gatcctgccc gccgcctgaa tctcgcggta gaggtcgagg
     1681 tggcccggtc cgttgggtgc ttctcctggc gcaggggtgt actgaacgac tttgaggtat
     1741 ggcagcgaga ggagccgggg caggtgctgc cgcgcatcgc cgccgtcgag gtggtaccag
     1801 atagccccga aggcttcgcc gtacacttcc agctccggca cgatgaaccg gtcgaacatg
     1861 gcaggggaca gcatgcagga cacgtcggac tgcgtgccat agtaaggttc gggcgcccag
     1921 aattgcatcc atcccgcatt gccgtaccag aactcgtggc gcgaccgcac atggtcaccc
     1981 agctccatcc ggagccgtag ctgttccttc gcgccggtaa cgatggcttc ccgcatccag
     2041 tcggcgtggt ccacaagggc gaggagaaac gtttcggtgc ccatgagcgt cgaggcgagg
     2101 tcgtttgcgg gaagtaggag cggctttccc acaaggaagt catctcgccc ggcgaaatcg
     2161 agcgctgcgt cgagtagcgc gcgatactcc gccacccacg ggttgtcggg ttcgtaccgg
     2221 aacggcggcg gaagggagaa atccacgtcc ttcgtatcga accagatggt atcctgcgcg
     2281 aaccggggcg taccgccaag gcagagaacc cagccggcca tcatgaggca ggacggcacg
     2341 gcctcaccac cccaccaggt gttctccagt tgcgcctcca tggattggag tacccagccg
     2401 gggtccagcc atctatcccg cggggcggac ggtgcaggcg ggtgaggaaa cgacctgccg
     2461 ctgggcgcag tcaccgcgat gcacgggcgg tcgagcagtt cgtggtgcca gagcgcggtc
     2521 aggcgggcct tcgcgtcggg ccagtcgggt ttgtatttca tctgtccttc cgggggcgag
     2581 gcgaaagtcc gtccgacatt ctaccgggtt tcccgcccaa cctccagcgc acggggacgc
     2641 aggctgttca ggaacgcgat ggtctccgcc gcccggaaat cggggtacgt ggtgctgagc
     2701 ggcgcgtaac tgccgcgttc atagcgcagg gtgatctccg cccagattcc gtcccgcagg
     2761 tagatgcggt atgccgaggg ttttccgctt gccagcacca gcgtaagagc gcacaggtat
     2821 ccggggtcta tgttcaccct ccgtgcaccc tcggcagtat gccagaagcg ctccagttcg
     2881 tttgaacgca gcttgatatc ggcaaggtgc tccgggcgga ctggctccgc aaacgcgagg
     2941 atacgttttg tgagacccgc gcccatttcc tcggcgtagt actcggtata gtcgtcgaag
     3001 caaaaggccg gtcccattgt gctgatcggc ccccagacgt cctccaaccg ggcgacgatt
     3061 ccggcttccg ccacttcggg cgaccacgtg accgcggcga tgaggaggac cggcggcgcg
     3121 ggacggggga tgctcacgcc tatctcctca cccggacggt ttgcacgcgc accttcggca
     3181 ggtcctgctc caggtacgcg cggtggaagg cgtagataag cccgtcctcg ccgccgatca
     3241 gcatgtccag ccggccgtcg ccatcccagt ccaccggttg cggacaggcg gcgtgggcgg
     3301 cgaggcggag accggggtcg ggcaccatct caccgcggaa cgcgaaccgg tcgccgccga
     3361 tgttctcgta caggaacgcg tttcccgctt cggtttcgac tgtgtgcatg cggttgtgcg
     3421 tgggttcggc gactccgcgg atgaggtcga ggtcgccgtc gccatcccag tcggcgaatg
     3481 cgaggcgata gcgcccgctg tggccgttgc gcatggggtt gaggaggagc ggttcgccct
     3541 gttcgttgag gaacactcgt tgcaacgcgc cgatgcgctt cccctgcggc gtcacgatac
     3601 cgggaaacag cgcgaggtag ccctcgtgat cgagggagac gtaatccgtc actccgtcgc
     3661 cgttccagtc gagcacctcg gtgcgggtgc gccactgggt aacgagttcg ccgttctgag
     3721 ggtcccacca cgtccacgcc ggtttgatgg tactggccgg gtcggcgaac cggaccgcgt
     3781 cctccgcgcc aaggaccagc ccgtcagcgc cgttttccag ggcggggtag aagcggtgtt
     3841 tcccgagcgc atcggcgagg atgaggtcga gcaggccatc cccgttccag tcggtgattt
     3901 ccggcgccac gtagccccag cgcgcttctc cggggccctg tatgcttccc cacgcgcccg
     3961 gaacgatgcg gaacggccgc ccttccgaga gcacgcggcg ggggaggtcg aacacccatg
     4021 cgggttcgcc gggcagggac aaccactgcc atgtctcgcc gttggcgttg cccatgatga
     4081 tgtcgggctt gccgtcgcgg ttcacgt
//
LOCUS       DAOSOO010000249         4096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21929_length_4096_cov_5.632022, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000249 DAOSOO010000000
VERSION     DAOSOO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21929_length_4096_cov_5.632022"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PLT86_13155"
     CDS             <1..1780
                     /locus_tag="PLT86_13155"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT37603.1"
                     /translation="RSRQPNMPIHCTEFWTAWYLTWHRDRRWLNRSPEHLAYHTWRVL
                     AEGGSGYNYYMWYGGTNFAYTTMYLQTTSYDYDAPLSEAGGIGRRGRLTIPIAHFAQA
                     FAHILADSDASCEKRADGIEVWRRSNSQGTLLFCKNPSDRPVTLPAATEDTKELGPVH
                     VPPESVAPIVAGATIAPCSAVRLVTACPVHGIYRVGACYIIVLMGARGSSIHCTLVHG
                     STGRGFLHTLKGDEPVVAGDWNAGERIRILVVPDAFSGRVWCAGDRLVIGASYARAVP
                     EGVEIQHTAATVSTGAWTVTESAITHVPSRAPRLPAPPTLSPWDSCIENAHLPCKKPR
                     TEPNGWVGSLRPVPRDLVGDHEGYGWYRAVFHSREARTALLHIEAVSDRALVFVNGGH
                     VSTSIEPVEDRRFQPSVTVPVELKAGENVIAVLVDNLGHVKGDWQIGEVGKRGSPTMR
                     DDLKGILGRVWVDADEVLEWEMHAKLLGEHEEWYRPGANAPWGSQHQPSPVSWYRASF
                     TMDTSVRRPLWVRVTGLNKGCIWVNGYHIGRYWNINGHTDYYVPECWLADSNTIVLFE
                     ETDATPEAVALVWDDVATLVESTVLK"
     gene            1943..2683
                     /locus_tag="PLT86_13160"
     CDS             1943..2683
                     /locus_tag="PLT86_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT37604.1"
                     /translation="MILAAIQLDTVAMEVNVNVHKALHWTRTAFENGAKYVFLHEGLT
                     ADYAPDPMKYGRALESVEVFGFAMLAKRYGGYVALGLNEVYKGAPYISCVYLDGNGVI
                     DVYRKSYLWSLPDRDNYIGYIHGFRQELGILGHGDGPRNVRVGDLLIGSLICADGNNP
                     QAWEPFRKEPPDIVFYQNNRGNVDEARNPDFCREINRPMVCTNRVGFSYYHFQLGGTR
                     FIRRDGTIAVAANTEGKEQAIYARWQDL"
     gene            2712..3845
                     /locus_tag="PLT86_13165"
     CDS             2712..3845
                     /locus_tag="PLT86_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37605.1"
                     /translation="MTHRERYIQALTFGNPDKIPFSPGGPREKTLKRWHAEGLPENVN
                     YMEALYDILGFHPEPAEKPAPGLNVDFRMIPQFEEKVLEHKNGHYIVQDWMGAITEIS
                     DEYDYTYIRAARDFVTRKWHRFPVQNRQDFEEKIKWRYDPKAPGRFPPDFEERCEKNR
                     DRDTVAAIHFNAPFWQLREWCGFEGLCTLMIDDPEFVEEMADFWTNFVLETMRPILQR
                     VQLDSVGMSEDMAYKSFSMISPAMVRRFLKPAYDRWVPEIRRSGCPIIDMDSDGFIGE
                     LIPIWIESGINVCDPIEVAAGNDINKFRARFGNRMAYKGGIDKRAIAAGGETMRGELE
                     RVVPPMLKTGGFIPGCDHGVPHDISWPNFIEYSRLLAQMTGWL"
     gene            3889..>4096
                     /locus_tag="PLT86_13170"
     CDS             3889..>4096
                     /locus_tag="PLT86_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37606.1"
                     /translation="MPDAAPFPVRVSSDRADAMYRVGEAVSFLVEKVGEGSPDDAVVT
                     VTWNGGAVVHTEQVKIGALPQMITV"
BASE COUNT          854 a         1313 c         1169 g          760 t
ORIGIN      
        1 ccgttcccgg caaccgaaca tgcccatcca ctgtaccgag ttctggacgg catggtatct
       61 cacgtggcat cgcgaccgcc gctggttaaa ccgctctccg gaacacctcg cctaccacac
      121 ctggcgggtg ctggccgaag gaggttcggg gtacaactac tacatgtggt atggcggcac
      181 caacttcgcc tacaccacca tgtacctcca gacaaccagc tacgactacg atgctccgct
      241 cagcgaggcc ggaggaatcg gcaggcgtgg ccggttgacc attcccatcg cgcatttcgc
      301 tcaggcgttc gcgcacattc tcgccgattc agacgcctcg tgcgagaaac gcgcggacgg
      361 catcgaggtc tggcgacggt ccaactccca gggcacgctt cttttctgca aaaacccgtc
      421 agaccgcccg gtcaccctgc ctgccgccac ggaagacacg aaggaactcg gcccggttca
      481 cgtgcccccg gaaagcgtcg cacctatagt tgccggcgct acgatagcgc cttgttccgc
      541 cgttcgtctc gtcaccgcat gcccggtaca cgggatttac cgcgtcggcg cgtgctatat
      601 cattgtcctg atgggcgcgc gcggctcttc tatccattgt accctcgtgc acgggtctac
      661 cgggcgcggt ttcctccaca cgctgaaagg agatgaaccc gttgtcgcag gggattggaa
      721 cgccggagag cgcatccgga ttctggtcgt tccagacgct ttctccggcc gtgtctggtg
      781 cgcgggtgac aggctcgtga ttggcgcgag ttacgcccgt gcggtgcccg aaggcgtgga
      841 aatccagcac actgctgcaa ccgtctccac tggcgcgtgg acggtcaccg aatccgcgat
      901 cacgcacgtt ccatcccgag ctccccgtct ccccgcccct cccacgctca gtccatggga
      961 tagttgcatc gagaatgccc atctcccctg taagaaacca cgtacggaac caaacggatg
     1021 ggtgggcagc ctccggccgg ttccacgtga tctagtcggt gaccacgaag gctacgggtg
     1081 gtaccgtgcg gtctttcatt cacgtgaggc gcggaccgca ctcctccaca tcgaggcagt
     1141 cagtgatcgt gccctggtat ttgtcaacgg cggacacgtg agcacctcaa tcgaaccggt
     1201 ggaagaccgg cgcttccagc ccagcgttac cgttccggtg gaattgaaag ccggagagaa
     1261 cgtgatcgca gttctggtgg ataacctcgg gcatgtgaag ggcgactggc agattgggga
     1321 ggtgggcaaa cgcggcagcc ccaccatgcg cgacgacctg aagggcatcc ttggccgcgt
     1381 ctgggtggat gcggacgaag tgctggaatg ggaaatgcac gcgaagcttt tgggcgaaca
     1441 tgaggaatgg taccgccccg gtgcgaacgc accgtggggg tcgcagcatc aaccctcgcc
     1501 ggtgtcctgg taccgcgcct cgttcacgat ggatacctct gtcaggcggc ctctctgggt
     1561 gagggtcacg ggcctcaaca aaggctgcat ctgggtgaac ggctaccaca taggccgcta
     1621 ctggaacatc aacggccaca ccgattacta cgtgccggag tgctggctgg cggattcaaa
     1681 cacgatcgtt ctgtttgagg aaaccgacgc gacgcctgaa gcggtcgcgc ttgtgtggga
     1741 tgacgtggca acgctggtgg agtcaaccgt cctcaagtag cgtccctcgc acctgcgccg
     1801 ctcacgccgc ggagcttccg cgaacgttcg gatcttctct gactgggcct gccttgcgcc
     1861 gcgccccgcc cttgttcccc tcattcacgc ggactgatat tccttgcggc acgcctgcgc
     1921 ggagacatca gggagacgca tcatgatact cgctgcgatc cagctcgata ccgttgccat
     1981 ggaagtgaac gtgaatgtcc acaaagccct ccactggaca cgaaccgcgt tcgagaatgg
     2041 cgcgaaatac gtgtttctcc atgaggggtt gactgccgat tacgctcccg accctatgaa
     2101 gtacggtcgc gcacttgaaa gcgtggaagt cttcggcttc gccatgctcg cgaagcggta
     2161 cggtggttat gtcgcgctcg ggctcaacga ggtctacaaa ggcgccccct acatctcatg
     2221 cgtgtacctc gacggcaacg gcgtcataga cgtctaccgc aaaagctacc tctggtccct
     2281 gcccgaccgc gataactaca tcggatacat ccacgggttc cggcaggaac tcggtatcct
     2341 ggggcacggg gacggcccgc gcaacgtccg ggtcggcgac ctgctcatcg gctcccttat
     2401 ctgcgcggac gggaacaacc ctcaagcgtg ggaaccattc cggaaggaac caccggacat
     2461 cgtcttctac cagaacaacc gcggaaacgt tgacgaagcg cgcaaccccg atttctgccg
     2521 cgaaatcaac cgcccgatgg tctgcaccaa ccgggtgggc ttctcctatt atcatttcca
     2581 gctcggtgga acacgattca tccggcggga cggcaccatc gccgtcgcgg caaacaccga
     2641 aggcaaagag caggcaatat acgcccgctg gcaggatctc tgagccaact gcaaaccgaa
     2701 aggctgatca gatgacgcat cgcgaacgct acatccaagc cctcaccttc ggcaacccgg
     2761 acaaaatccc cttcagcccc ggtggcccgc gcgaaaaaac gctcaaacgc tggcacgcgg
     2821 aaggcctgcc cgaaaacgtc aactatatgg aggcgctgta cgacatcctc ggtttccatc
     2881 ccgaacccgc ggaaaaaccc gcccccggcc tgaacgtgga tttccgcatg attccccagt
     2941 tcgaggaaaa ggtgctcgaa cacaaaaacg ggcactacat cgtgcaggac tggatgggcg
     3001 ccatcaccga aatctccgac gaatacgact atacctacat ccgcgccgcg cgcgattttg
     3061 tcacgcgcaa atggcaccgg tttcccgtcc agaaccgcca ggatttcgaa gaaaaaatca
     3121 aatggcgcta cgacccgaag gcccccgggc gcttcccccc ggatttcgag gagcgatgcg
     3181 aaaaaaaccg tgaccgcgac accgtcgccg ccatccactt caatgcaccc ttctggcaac
     3241 tgcgcgaatg gtgtgggttc gagggccttt gcacgctcat gatcgacgac cccgaattcg
     3301 tcgaggagat ggcggacttc tggaccaact tcgtcctgga aaccatgcgc cccattctcc
     3361 agcgcgttca gctcgattcc gttggtatgt cggaagacat ggcctacaaa agcttcagca
     3421 tgatctcacc cgccatggtc cgccggttcc tcaaaccggc gtacgacaga tgggtgcccg
     3481 agattcgcag aagcggctgc cccataatcg acatggactc agatggcttc atcggagaac
     3541 tcatcccgat ctggattgaa tccggaatca acgtctgcga ccccatagaa gtcgccgcag
     3601 gcaacgacat caacaaattc cgtgctcgtt tcggcaaccg catggcgtac aaaggcggca
     3661 ttgacaaacg cgccattgcc gccggaggcg aaacaatgcg cggcgagctt gaacgcgtcg
     3721 tgccgccgat gctcaagacc ggcggcttca tccccgggtg cgatcacggg gttcctcacg
     3781 atatctcatg gcctaacttc atcgaatatt cgcggcttct cgcgcagatg accggctggc
     3841 tgtaaacccc gattccatgt tcgtctcagc gagaggaagc tcacggaaat gccagacgct
     3901 gccccgttcc ccgtacgcgt atcgagcgac cgcgcggacg ccatgtaccg agtcggcgag
     3961 gcggtttcct tccttgtcga gaaagtcggc gaaggctccc cggacgacgc tgtcgtaacc
     4021 gtcacctgga atggcggggc agtggttcat accgaacagg tgaaaatcgg tgcgttgccg
     4081 cagatgatca ctgtgg
//
LOCUS       DAOSOO010000250         4088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_21980_length_4088_cov_4.980412, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000250 DAOSOO010000000
VERSION     DAOSOO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21980_length_4088_cov_4.980412"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLT86_13175"
     CDS             complement(<1..121)
                     /locus_tag="PLT86_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37607.1"
                     /translation="MALKIGIVGMGGIGNTHARVYMDDPLAEIVAVCDIIKARA"
     gene            complement(185..1105)
                     /locus_tag="PLT86_13180"
     CDS             complement(185..1105)
                     /locus_tag="PLT86_13180"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOT37608.1"
                     /translation="MAFPGSGPIWFDGAFVPWEEAKIHVLSHVAHYGSGVFEGIRCYG
                     TKNGPAVFRMREHVRRMFDSAKIYRMPIPFTQEEIEAAILETIRRNGLDACYIRPLVF
                     RGYGQLGVSPLNCPVNVIIAVWEWGKYLGPEALEQGVNVCVASWNRPAANTFPSLAKA
                     SGNYLNSQLVKLEAMANGYDEGIVLDTSGYVAEGSGENIFVVRDGILFTPPTSSSLLP
                     GITRNSVITLARELNYTVREEQIPREALYIADEVFFTGTAAEITPIATIDKVPIGSGK
                     RGPVTKEIQDALFGILDGTIADRHGWLTPV"
     gene            complement(1303..1944)
                     /locus_tag="PLT86_13185"
     CDS             complement(1303..1944)
                     /locus_tag="PLT86_13185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT37609.1"
                     /translation="MGEIKDGQTLLFIGDSITDCGRRGPNYPLGDGYPALFRDLMTAR
                     WPERNIRYINKGIGGNRVTDLQMRWEDDVMRYKPDWLSIKIGINDEHSFVADPNNGVS
                     PQRFREVYDSILQRTFSVWKPNLILISPFYISQDTVSGTHRTKILQLIPEYIAVVKDM
                     AAKYNAIYVPLHDIFQKHLEYRDADTFCPEPVHPYRSGHIIIARAIMHAIDES"
     gene            complement(1966..2514)
                     /locus_tag="PLT86_13190"
     CDS             complement(1966..2514)
                     /locus_tag="PLT86_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT37610.1"
                     /translation="MTRNTLSAEDVIRALGLVPHPTEGGWYVETYRCPEEVNSTDLPA
                     RYRGNRSFSTAIYYLLTSETFSRLHRLASDEVFHFYLGDPVVMVHLLPDGTSRRVILG
                     HDIAAGQKPQVIVPRGTWQGAHLREGGKWALLGCTVSPGFDYRDYEEGSRDALVAMYP
                     DQRETIDRLTREDEDRSPRSLP"
     gene            complement(2528..2734)
                     /locus_tag="PLT86_13195"
     CDS             complement(2528..2734)
                     /locus_tag="PLT86_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT37611.1"
                     /translation="MRKLYRSRTDKKIAGVCGGIAELYDIDPTIVRLIALLLILGYGA
                     GLIVYLVAWWLVPLAPEKPPDQQA"
     gene            complement(2745..3320)
                     /locus_tag="PLT86_13200"
     CDS             complement(2745..3320)
                     /locus_tag="PLT86_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOT37612.1"
                     /translation="MKRPRGSRHGVALVFALALLAAGTGACNSENPPSREGVAMVRVT
                     SSAFSEGNSIPALYTCEGNDVSPPLKWSGVPAETKSIALTCTDPDAPRGTFVHWVLFN
                     LPPSTDGLPEGVTPDTVPQGAVNGTNDFPKLGYGGPCPPRGAPHRYYFTVYALDTVLA
                     LHPGARLRDLTAAMEGHILAEGSLMGRYARK"
     gene            complement(3484..>4088)
                     /locus_tag="PLT86_13205"
     CDS             complement(3484..>4088)
                     /locus_tag="PLT86_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT37613.1"
                     /translation="GLANAVKALAADSAVFGKVTGDLSGGAMNQTFVNVLDTAGIVVR
                     SGLVSPLGWFLYEKLQPGTYRVRAEHATLSMSAETTLVLPMLPREIHLVLKPSVRVEN
                     VPHPGAFWVGQPFPNPGNPGTRIAFRVPSGSASGYLGARVFSVNGQVVRETHVPISGT
                     NGVFAWDGSDSSGRPVASGVYVVRLSFQGVTTARRVVIAR"
BASE COUNT          858 a         1234 c         1173 g          823 t
ORIGIN      
        1 ccgcgcgtgc cttgatgatg tcgcacacgg caacaatttc cgccagcggg tcatccatgt
       61 agacacgcgc gtgggtgttg ccgataccgc ccatgccgac gataccgatt ttgagtgcca
      121 tgacagtttc ctttgtgaga gaagagttct aaaaccagac ctgtgattcg ttgtcggagc
      181 accgtcacac cggagttaac cacccgtggc ggtctgctat cgtgccgtcg agaatcccga
      241 acagcgcgtc ctggatttcc ttcgtaacgg gtcctctttt cccggacccg atcgggacct
      301 tgtcgatagt tgcgatcggc gttatctccg cggcagtgcc tgtgaagaac acctcatccg
      361 cgatgtaaag cgcctcgcgc gggatttgtt cctcgcgcac ggtgtagttt agctcccgcg
      421 ccagcgtaat aaccgagttg cgcgttattc cgggcaggag ggaggagctg gtgggaggcg
      481 taaagaggat gccgtcccgc acgacgaaaa tgttctcgcc agatccttcc gcaacatacc
      541 cgctcgtatc cagaacgatt ccttcgtcgt acccgttggc cattgcttcg agcttcacaa
      601 gctggctgtt caggtaattc ccactcgctt tcgcgaggct cggaaaggtg ttcgccgctg
      661 gccgattcca cgacgcgaca cagacattga cgccctgctc cagcgcctcg gggccaaggt
      721 actttcccca ttcccacacg gcgataatga cattcaccgg gcaattgagg gggctgacgc
      781 caagttgccc gtacccccgg aagacaagtg gccgaatata gcaggcatcc agtccgttcc
      841 ggcgaatcgt ttccagaatc gctgcttcaa tctcttcctg cgtgaacggg attggcatcc
      901 ggtaaatctt cgcggagtcg aacatgcggc gtacgtgttc acgcatccgg aagacggcag
      961 ggccgttttt cgttccgtaa caacggatgc cttcgaaaac tcctgagcca taatgtgcaa
     1021 cgtgggaaag aacatggatt tttgcttctt cccacggaac aaatgcgccg tcaaaccaga
     1081 tcggccccga acccggaaac gccatcgtca gatatcttca gggatcaaac cttccctagg
     1141 acgcctttct ccgcccgcaa ccttgctcaa aacgacgtag ccattcgaaa actcgacata
     1201 acaggcgcgt acacgaggaa gaaaagtacg gctgcgcggc agaggggtca accggcgttc
     1261 ccggagcact tgcccaccag aaacgccggt ctgaaggata tttcaggatt cgtcaatcgc
     1321 gtgcatgatg gcccgcgcaa tgatgatgtg gccactgcgg tacgggtgca ccggctcagg
     1381 gcagaacgtg tcggcatccc ggtactcgag gtgcttctgg aagatatcat gaagcgggac
     1441 gtaaatagcg ttgtatttcg cggccatgtc tttcaccacc gcaatgtatt cggggatgag
     1501 ctggaggatc ttcgtccggt gagtgccgct cacggtatcc tggctgatgt aaaacgggga
     1561 gatcaggatg agattgggct tccacaccga gaacgtgcgt tgcagaatgc tgtcgtacac
     1621 ctcacggaac cgctggggcg acacaccatt gttcgggtcc gcgacgaagc tgtgctcgtc
     1681 gttgattccg attttgatgg aaagccagtc gggcttgtac cgcatgacgt catcttccca
     1741 gcgcatctgc agatcagtca cgcggttccc accgatgcct ttgttgatgt accgaatgtt
     1801 ccgttccggc cagcgcgccg tcatcaggtc ccgaaatagc gccggatatc cgtcgccgag
     1861 ggggtaattg ggtcccctgc gtccacagtc ggtgatgctg tcaccgatga agagcagcgt
     1921 ctgtccgtct ttgatttcgc ccattgtgaa ctccttttgt gtgtgctacg ggaggctgcg
     1981 agggctacga tcttcgtcct cgcgggtaag tcggtcgatg gtctctctct ggtccgggta
     2041 catcgcgacg agcgcgtcgc gcgacccttc ttcgtaatcc cgatagtcaa aacctgggga
     2101 aaccgtgcat cccagcaagg cccatttccc gccttcccgc aaatgggcgc cctgccacgt
     2161 cccgcgggga acgatcacct gcggcttttg tcccgctgcg atgtcgtgcc cgagaatgac
     2221 cctccggctg gttccatccg ggagcaggtg caccatcact accgggtctc ccagatagaa
     2281 gtggaacacc tcatcgctgg cgaggcgatg cagccttgaa aaggtctcag aggtcaaaag
     2341 gtagtagatc gccgtcgaaa aagaacggtt tccgcggtac cgtgcaggaa ggtccgtcga
     2401 attcacctct tccgggcaac gataggtttc gacgtaccac ccgccctccg tcgggtgagg
     2461 caccagtccg agagcacgta tgacgtcctc tgcggagagc gtattccgcg tcataggcac
     2521 aacggtatca cgcctgctgg tccggcggct tttcgggagc caacggaacc agccaccacg
     2581 ccacgaggta cacgatcaat ccggcaccat atccgagaat taacagcagc gcgatgaggc
     2641 ggactatggt gggatctatg tcgtaaagct cggcgattcc gccacacaca ccggcgatct
     2701 ttttgtccgt tcgcgagcgg tacaatttgc gcatggcaac ctccctattt gcgagcgtat
     2761 cgccccatga ggcttccttc tgccagaatg tgcccctcca tggcagcagt cagatcacgc
     2821 aaccgagccc cggggtgcag ggcaagcact gtatccagcg cgtagacggt gaagtagtac
     2881 cgatgcgggg ccccccgcgg cggacatggt cccccgtagc ccagtttcgg gaaatcgttt
     2941 gtgccgttga cggcgccttg aggaacggta tccggagtca cgccttctgg aagcccgtcg
     3001 gttgagggtg gaaggttgaa caggacccag tgaacgaacg tgcccctggg agcatcggga
     3061 tccgtgcacg tgagggcgat gctcttcgtt tcagcaggta ctcctgacca cttgagcggc
     3121 ggtgagacgt cgttgccctc gcacgtatac agagcaggga tcgagtttcc ttcactgaag
     3181 gctgagcttg tcacacgcac catggcaacc ccctcccgtg acggtgggtt ctcgctgtta
     3241 cacgcgccgg ttccggctgc aagcaaggca agcgcgaaga caagcgccac accgtgtctg
     3301 gaaccgcgag gccgtttcat tccattcccc cggtcgtcgg ccctcagcgc aggcatcgag
     3361 aatcccattg agcgtttgcg agtgatttcc acggaaaaga tgagcaaaaa cagcagcaga
     3421 agcaacgtat gacacaaagt gagaaacgcc gaaggggagg cgcaggagta ttggaaggca
     3481 ggcctaccgc gcgataacca ctctgcgggc cgtcgtcacg ccctgaaagg acagacgcac
     3541 cacgtacacg ccggaggcca cgggtctccc cgaactatcg ctgccgtccc acgcaaaaac
     3601 cccgttcgtt cccgagatcg gaacgtgcgt ctcccgcaca acctgtccgt tcacggaaaa
     3661 aacccgggca ccgaggtacc cggacgcact cccggaagga acacggaagg cgatacgcgt
     3721 accggggttc cccggattcg ggaaaggttg cccgacccag aacgctcccg ggtgaggtac
     3781 gttctccaca cgtacggacg gtttcagcac caggtggatt tcacgaggaa gcattggcag
     3841 caccagtgtg gtttccgccg acatactcaa cgtggcatgt tcggcgcgca cgcggtatgt
     3901 ccccggttgg agtttctcgt aaagaaacca tcccaagggt gagaccagcc cgctgcgaac
     3961 cacgattcca gccgtatcca agacattcac aaaggtctgg ttcattgccc ccccggagag
     4021 atccccggta acctttccga acaccgcgct gtcggccgcg agagccttca cggcgttggc
     4081 cagacccc
//
LOCUS       DAOSOO010000251         4063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22167_length_4063_cov_6.008982, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000251 DAOSOO010000000
VERSION     DAOSOO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22167_length_4063_cov_6.008982"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1651)
                     /locus_tag="PLT86_13210"
     CDS             complement(20..1651)
                     /locus_tag="PLT86_13210"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOT37614.1"
                     /translation="MASTTRPRARRAPRPALERPTLPEFLAASIQTRDGPFTFAGRAP
                     LVGICDALGNPAIRRVDILKATQIGMTTLAGFGYALWETAVNGRNVGYFLPTEWMARE
                     VVGERLRWAVGEKCGFDLGINANDGIARTGRGRIYVRGLITIHGALSVPLDVNLYDEV
                     DDLNPDHFLWARQRLDASHYAREIAFACGRFPGEGMDARFQSGTQHHWHVKCRRCRNA
                     DNVLELAFPDCVKRHGEGYALACTRCGGTLDAANGRWVAHYPDRQHMSFRVGALGMSA
                     LSLDRLMDEWDLALREKRLMAPFRCSKLALPDAAERQALTAQDLINACAPDAAISRPV
                     YVGVDTGDWCHIAMAEPVDGALVYRLFDRVPGEELVARLVMLASQHRIAGILVDERPE
                     GSLARAVCRAFPRVAMMQQFTAADYARRKTLAGETFGVLSFDREDTLGAWCDQVRMAP
                     PRVLFPRFVEGMPFVESAPGRHILTGAQRMEVEQDGFTIHRFRGGGVDNHYFMACVFA
                     WRMWAHSARGWREREDISLLGAITADAWRREATGA"
     gene            complement(1835..2029)
                     /locus_tag="PLT86_13215"
     CDS             complement(1835..2029)
                     /locus_tag="PLT86_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37615.1"
                     /translation="MIGFLRGKKTYFVAAGWIAWGIWTYAVERDPVAGTQRILEGVSL
                     ITLRAGVAKTGAGLEGHASS"
     gene            complement(2026..2301)
                     /locus_tag="PLT86_13220"
     CDS             complement(2026..2301)
                     /locus_tag="PLT86_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37616.1"
                     /translation="MTRRHRIRYQDWLMPLWAATTAALGSYIGVIRAISDLREEVRVV
                     RADYTATARALDGRLERAEAAATQTERRCESLVRHHEQAFHHRGRGE"
     gene            2529..3176
                     /locus_tag="PLT86_13225"
     CDS             2529..3176
                     /locus_tag="PLT86_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT37617.1"
                     /translation="MKTIAALTQIPGKPKRMRVEFRNGESVELGVDIIARHRLLAGRA
                     VSAEALRAAREEDARKEAEAAALKLLNRKNCSEQELRRALKANGFGERVVDEVVEKCR
                     GWGYLNDRRLAEMVVTDAIERRHLGPARIRQTLRKRGVDDALGASAQKRAADSEPSLV
                     EQALAALKGKERTYARLDRETSRRRMIAFLQRRGFSFDTIRAALEQLGEDAGGEE"
     gene            3559..3849
                     /locus_tag="PLT86_13230"
     CDS             3559..3849
                     /locus_tag="PLT86_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT37618.1"
                     /translation="MPHMYILKCADGTYYTGSTVDLELRVSQHQSGGGANYTKSRLPV
                     SLVYWEEYPRVDEAFAREKQVQAWSHAKKKALIDARNDTLSGLAKKVFRREA"
BASE COUNT          708 a         1364 c         1363 g          628 t
ORIGIN      
        1 ctgcctgccc gcggcccccc tacgcccccg tggcctcgcg gcgccatgcg tcggcggtga
       61 tggcgccgag gagggagatg tcctcccgtt cgcgccagcc acgggcacta tgcgcccaca
      121 tgcgccacgc gaatacgcac gccatgaagt agtgattgtc cacgccgccg ccgcggaaac
      181 gatgtatcgt gaacccgtcc tgttccacct ccatccgctg cgcgccggtc agaatatgcc
      241 gccccggcgc cgactccaca aacggcatcc cctccacgaa ccggggaaac aggacgcgcg
      301 ggggcgccat ccgcacctga tcgcaccacg cgccgagggt gtcctcccgg tcgaacgaga
      361 gcacgccgaa cgtctcgcct gccagcgttt tcctccgcgc gtagtccgcc gccgtgaact
      421 gctgcatcat ggcgacgcgg gggaacgccc ggcaaacggc gcgcgcaagg ctgccctcgg
      481 ggcgctcgtc cacaaggatg ccggcgatgc ggtgctgcga agccagcatc accagccgcg
      541 ccacaagctc ctcccccggc acgcggtcga acaggcgata gacaagcgcg ccatccaccg
      601 gctccgccat cgcaatgtgg caccagtcgc ccgtatccac gccgacatac accgggcgcg
      661 agatcgccgc atccggcgcg caggcgttga tcaggtcctg cgcggtgagc gcctgccgtt
      721 ccgccgcgtc gggaagggcg agctttgagc accggaacgg cgccatgagc cgcttttccc
      781 gcagggcaag gtcccattcg tccatgagcc ggtccagcga cagcgcggac atgccgagtg
      841 cgccgacccg gaagctcatg tgctgccggt cggggtagtg cgccacccac cgtccgttcg
      901 cggcgtccaa tgtcccgccg catcgggtac aggccagcgc gtatccctct ccgtggcgct
      961 tcacgcagtc cgggaacgca agctccagca cattatcggc gttccggcat cggcggcatt
     1021 tgacgtgcca gtggtgctga gtgccgctct ggaaccgcgc gtccatccct tcgccgggaa
     1081 agcggccgca ggcaaacgcg atctcccgcg cgtagtgcga agcgtccagc cgttgccgcg
     1141 cccagaggaa gtggtccggg ttgagatcgt ccacctcgtc gtacaggttc acgtccagcg
     1201 gcacggagag ggcgccgtgg atggtgatga gcccccgcac gtagatgcgc cctcggcccg
     1261 tccgcgcgat accgtcgttg gcgttgatgc cgaggtcgaa cccgcacttc tccccaaccg
     1321 cccagcgcag gcgttcgccc accacctccc gcgccatcca ctcggtgggc aggaaatagc
     1381 caacgttccg gccgttcacc gcagtctccc agagtgcgta gccgaacccc gccagcgtgg
     1441 tcatgccgat ctgcgtggcc ttgaggatgt ccacacggcg gatggctggg ttgcccagtg
     1501 cgtcgcagat gcccacaagc ggggcacgcc cagcgaaggt gaacgggccg tcccgcgtct
     1561 ggatggatgc ggcgagaaat tccggcagcg tgggacgttc cagcgcgggc cgcggtgccc
     1621 ggcgtgctcg tggacgggtt gtggatgcca tggtttcctt tctgacatgt ggtgttgata
     1681 tcggaggtgg tgccccggcg cctcgatacc acgaaccggc cagggaccgc acgcaacgga
     1741 gggcacgaca gagcgtgccc ctccagtcgt ttctggttcg acacacgccc gcgcggccct
     1801 tcgacaggct tcctgcgccg tgccgcggag aaggtcacga cgaagcgtga ccctccaacc
     1861 ctgcacccgt cttcgcgacg ccggcgcgca gggtgatgag gctcacgccc tcgaggatgc
     1921 gctgggtgcc ggccaccggg tcgcgctcca cggcatacgt ccagatgccc catgcgatcc
     1981 agcctgccgc cacgaaatag gtctttttgc ccctgagaaa tccgatcatt cgcccctccc
     2041 ccggtggtga aatgcctgtt cgtgatgcct gacgagtgat tcgcaccggc gctccgtctg
     2101 cgtggcggcc gcttcggcgc gctccagccg cccgtcgagc gcccgcgccg tggcagtgta
     2161 atccgcccgg acgacgcgca cctcctcgcg aaggtcgctg atggcccgta tgacgccgat
     2221 gtagctcccc agcgccgccg ttgttgccgc ccagaggggc atgagccagt cctgatacct
     2281 gatcctgtgt cgccgcgtca acgcgaacct ccttcctgaa atggcttccc gtggtggtgc
     2341 cggagtgatt tcccgagacg tattccccgg ccgtttccag gtgaggcaaa acgtcgtctt
     2401 cgtgtcaaga ccctccgcaa gttttttttc gcggcccgga tgaggcggca gaccggcgtc
     2461 agcgcgggga cactcacttg ccgcggggca gggaagcagg cattcttaga gggaaaggag
     2521 cgcccgccat gaagacgatt gccgcgctga cacagatacc gggcaagccg aaacggatgc
     2581 gcgtggagtt ccggaacggc gaatcggtgg agctgggggt ggatatcatc gcccggcacc
     2641 ggctgctggc cggccgcgcg gtgtcggcgg aggcgcttcg ggccgcccgg gaagaggatg
     2701 cccggaagga ggccgaagcc gctgccctga agctgctgaa ccgcaaaaac tgctcggagc
     2761 aggagctgcg ccgggcgctg aaggcgaacg ggttcggcga acgggtggtg gacgaggtgg
     2821 tcgagaagtg ccgcggatgg ggctacctca acgaccggcg gctggcggag atggtggtga
     2881 cggacgccat cgaacggcgc cacctcggtc ccgcgcggat acggcagacg ctccggaaac
     2941 ggggcgtgga cgatgcactg ggtgcctcgg cgcagaaacg cgctgcggat tcggagccat
     3001 cactcgtgga gcaggcgctg gcggcgctga aggggaagga gcgcacctac gcccggctcg
     3061 accgcgagac atcccggcgc cggatgatcg cgtttctgca gcggaggggg ttttccttcg
     3121 ataccatccg cgcggcgctg gagcaactcg gcgaggatgc gggcggagag gagtgagggg
     3181 gcgtggggct cctcgataca acgtctgctg cgcagccgtt actcggagac ccgcgcgcca
     3241 atccggtcgt cgagtggcgt gccggcagag gaacggaagc gaagcgacgt tcccggcgcg
     3301 ctgtatcgag acgacccggc cggagcaatg caatgccaca catgtacgtc ctgaagtgcg
     3361 cgtgtgggga atgacagcgc aaggcgccca ccgccacgca cccgggcggc gcggctcctc
     3421 gatacgacgt ctgctgcgca gccgtcactc ggagacccgc gcgctaatcc ggtcgtcgag
     3481 tggcgcgccg gaagaggaac ggaagcgaag cgacgttccc cgttcgttgt atcgagacgc
     3541 accggccgga gcaatacaat gccacatatg tacatcctca aatgcgcgga cggaacgtat
     3601 tacacgggca gcaccgtcga tctcgagttg cgcgtaagcc agcatcagtc tggaggtggc
     3661 gcgaattaca ccaagagccg attgcccgta tcccttgtat actgggaaga ataccctcgt
     3721 gtggacgaag cattcgcgcg cgagaagcag gttcaagcgt ggagccacgc gaagaaaaag
     3781 gcgctgatag acgcgaggaa cgacacgcta tccggtctgg cgaagaaggt ctttcgacgc
     3841 gaagcctgaa ctgcacgctc ccgcgcggat ctccgagcgg tgtggaggcc acgaacggtt
     3901 gggcctggct ctccccggtc atctcgatac aacctgcgct cgcgcgcagg ccactcgatg
     3961 accggtcctt gcgcggcacc ctgtacggtc gtcgagtggc gcgccggcag aggaacggaa
     4021 gcgaagcgac gttcccggcg cgttgtatcg agacgacccg acc
//
LOCUS       DAOSOO010000252         4052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22244_length_4052_cov_6.065799, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000252 DAOSOO010000000
VERSION     DAOSOO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22244_length_4052_cov_6.065799"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PLT86_13235"
     CDS             complement(<1..1386)
                     /locus_tag="PLT86_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT37619.1"
                     /translation="MRTYVGVVVSGLALLASSCAKQGPAVVPVQRVEMVAAADLPVLG
                     TRALPTDPQKFSFVILGDKSGGLDRNWPLFDRAVQEINLLKPDFIITVGDQIEGSNDS
                     ASVVAQWELFRSHAGKFEAPLFMLPGNHDAADLMGYEYWKRNVGRTYYDFMYKGCYFL
                     VLNTQEASREFPQFGEAQIAWAVDALQRHPNPRHTFVFMHYPAWFASDPERQRGGISA
                     EWQRIETALGNRKYSVIAGHTHNLMWATRDGNRYLVHGATGATLTPSPVKQVGAFHHY
                     AKVTVEADQAHIAIVEPGNIWPETIAPLEFQRNIGRLVRIQSSRRDLADGRIAMDVTA
                     ELNNHVGDTVSVALIPVLGAAGVWAPSADSLVSVLPDGGTDSLTLTFVGGRDNWYPTP
                     AWKMVVRYQGKEVDTYGPSPLNPFSESRATVLPEWRALGTFPVGPINRDVMPGNPRAG
                     VPGMFVPRAPDA"
     gene            complement(1430..2893)
                     /locus_tag="PLT86_13240"
     CDS             complement(1430..2893)
                     /locus_tag="PLT86_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT37620.1"
                     /translation="MEYVVEPEKRVPVVYDVDVCVAGAGVSGVFAAIAAARNGARTVV
                     VDRFGSVGGNVGPGMIVNGQMLPGSPHPDVPFTKTVYPGLFGLAEEFINRYAALGGGL
                     LQPFTKGANFSRGASVASYLLQEMLEESGASILLSTFVADPIKEGDRVCGVFVENKSG
                     RAAVRAKVVIDATGEADVARRAGAPILLPKNEYTAIDGHAPTGMGTYYVVGGIAWDRF
                     RAAQKGNEATEDDIAWGTKTIGAALTKAHSYVLPYLRKAVEAGEYEPVAKVMIGGKSI
                     SVQGGGVGQFEGNELGHGQVQVERTEEVNAGDGLHMSALGAAVQAYAFEKVQFFSRYV
                     PGFENAYLITVAPFLGSRGGPCILGEYTLTMDDCLNGRRFDDVIYLYGEFRALRKTAE
                     AGAPKWVDVPYRVLVPQGVDGLLATGRSASCIPDTLLRNRLAVKVMGEACGIAASMCA
                     AKGVEPRKLEVRELQNALLDAGFYLGDRARLKELSLT"
     gene            complement(2917..>4052)
                     /gene="rlmD"
                     /locus_tag="PLT86_13245"
     CDS             complement(2917..>4052)
                     /gene="rlmD"
                     /locus_tag="PLT86_13245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT37621.1"
                     /translation="GQCGGCTWQQWEPGIQHEMKRRIVREALMRAMPDAAIEVLPVVA
                     AGPEFGYRNKMEFTFGKDETGAVTLGLHRVGRFDATFDVKRCWIAPEGMNAVRAWVCE
                     WARRNALVPYDPRRFAGALRHLVLREASATGEVMANLVCATEEVAGLDELSEGLLREL
                     PQVKSLLFSVNTRRGDTAHAETTVLLSGRPAISERIGDMEVTVSPISFLQTNSAGAER
                     LYSVVSEVAALSGEERVLDMYCGIGLIGISLAPRAREVVGLEQVPSAVQDANAMRERL
                     GYTHVQFRQGDAEAVLPAWADRGERFDVVIVDPPRAGLHPKALAALVRMSPRLIVYVS
                     CNPVTLAANLADLSSSGYRIGAAHPVDLFPHTPHVETVVRLEK"
BASE COUNT          783 a         1427 c         1076 g          766 t
ORIGIN      
        1 tgcatcaggt gcgcgcggta caaacatccc gggtacgccc gcccgaggat tgcccggcat
       61 gacatcgcgg ttgatggggc ccaccggaaa cgtgcccagc gcacgccact ccggcagaac
      121 cgtggcacgg ctctccgaaa acgggttaag gggcgacggc ccgtaggtgt cgacctcctt
      181 cccctgataa cggacgacca tcttccacgc cggcgttgga taccagttgt cgcggccccc
      241 gacaaacgta agcgtcaggc tgtccgtccc gccgtccggc aggaccgaaa cgaggctgtc
      301 cgcagaagga gcccagactc ccgcggcccc gaggacgggt atcaacgcta cggacaccgt
      361 gtcgcccaca tggttgttca gctccgccgt aacgtccatg gcgatccgtc cgtcggcgag
      421 gtctcgcctg ctcgactgaa tccgcacaag gcgcccgatg ttccgctgga attccagcgg
      481 cgcaatcgtc tccggccaga tgtttccggg ttcaacaatc gcgatgtgcg cctgatccgc
      541 ctccacagtg actttggcgt aatggtgaaa cgcaccgacc tgcttcaccg gactcggcgt
      601 gagcgtggct ccggtggcgc cgtgcacgag gtaacgattt ccgtcacgcg ttgcccacat
      661 gaggttgtgc gtgtgccccg cgatgacgct gtacttcctg tttcccagcg ccgtttcgat
      721 tcgctgccat tccgcggaaa tcccaccccg ctgccgctcc ggatcactcg cgaaccacgc
      781 cggataatgc atgaacacaa acgtgtgccg cgggttgggg tgcctctgga gcgcgtccac
      841 cgcccacgcg atctgcgcct cgccgaactg agggaattcg cgggaagctt cctgcgtgtt
      901 cagcacgaga aagtaacatc ctttatacat gaagtcgtaa taggtccgac ccacgttacg
      961 cttccagtac tcgtacccca tcaggtcggc cgcatcgtgg ttgcccggaa gcatgaacag
     1021 aggcgcctcg aactttcccg catggcttct gaaaagctcc cactgcgcca ccacgctggc
     1081 gctgtcgttc gatccttcga tctggtctcc aaccgtgatg atgaagtcgg gcttcaggag
     1141 attgatctcc tgtacggccc gatcgaaaag cggccagttc cggtccagtc ccccggactt
     1201 gtcgcccagg atgacgaagc tgaacttctg cgggtcggtg ggaagtgccc gcgttccaag
     1261 gaccggcaga tccgcagcgg caaccatttc cacacgctgc accggcacca ccgccgggcc
     1321 ctgcttcgcg caggaacttg ccagaagggc cagtcctgaa accaccacac caacgtatgt
     1381 tctcatgtct ctgtgctcct tttgtgagac tttcctcttg tccacggaat caggtcaacg
     1441 aaagctcttt caagcgtgcg cgatccccga ggtagaaccc cgcatccagc agtgcgttct
     1501 gaagctcgcg gacttcgagc tttcgtggtt cgacaccctt ggccgcacac attgaggccg
     1561 cgattccgca tgcctcgccc atcaccttca ccgcaagccg gtttcgcagc aacgtatccg
     1621 gaatgcacga cgcggatctc cccgtcgcaa gcagaccgtc cacgccctgt ggaacaagca
     1681 cgcggtacgg cacatccacc catttcggcg ccccggcctc tgccgtcttc ctcagcgccc
     1741 ggaactcgcc gtacagataa atcacgtcgt caaagcgccg tccgttcagg caatcgtcca
     1801 tcgtcagtgt gtattcgccc agaatgcacg gcccgccccg cgatcccagg aacggggcca
     1861 cggtgatgag ataggcgttt tcgaacccgg gcacgtatct gctgaagaac tgaacctttt
     1921 cgaacgcgta tgcctgcacg gccgctccaa gggccgacat gtggaggcca tcccccgcgt
     1981 tgacctcttc cgtccgttcc acctgaacct gtccgtgccc cagctcgttg ccctcaaact
     2041 gcccgacccc gcccccctgg acggagatag acttcccgcc gatcatcacc ttcgccacgg
     2101 gttcatactc gccggcctcc acggcctttc gcaggtaagg aaggacatac gaatgagcct
     2161 tcgtaagcgc cgccccgatt gtcttggttc cccaggcaat gtcgtcttcc gtggcttcgt
     2221 tccccttctg ggcagcccgg aaacggtccc aggcgattcc cccgaccacg tagtacgtgc
     2281 ccatgcccgt gggtgcgtgc ccgtcaatcg cggtgtactc gtttttcgga agtagtatcg
     2341 gcgctccggc ccgccgcgca acgtccgcct caccggtggc gtctatcacg acctttgccc
     2401 gaactgccgc acggcctgat ttgttttcca cgaaaactcc acacacccgg tcgccttctt
     2461 tgatcggatc tgccacaaag gtcgagagga gaatcgacgc gccgctttcc tccagcattt
     2521 cctgcagcag atacgaggca acgctggctc ctcgcgagaa attggcgcct ttcgtaaacg
     2581 gctgcagcaa tccgcccccg agggccgcat aacggttgat gaattcttcc gccagcccga
     2641 acagaccggg gtagaccgtt ttcgtgaagg gaacgtccgg gtgtggcgat ccgggcagca
     2701 tctggccgtt gacgatcatg ccgggcccga cattcccgcc aacgctcccg aatctgtcta
     2761 ccaccaccgt tcgcgcaccg ttacgcgccg cggcgatcgc ggcaaaaacc ccactcacgc
     2821 cagcaccggc gacgcagaca tcgacgtcat acaccaccgg tacacgtttt tccggttcaa
     2881 cgacgtattc catgccgcat cctttctgcg cggagcttac ttttcgagcc gcacgaccgt
     2941 ctctacgtgc ggcgtatggg gaaacaggtc caccggatga gccgcaccga tccggtatcc
     3001 cgaagaggac agatcagcca aattcgccgc cagtgtcacc gggttgcacg aaacatatac
     3061 aattaagcgg ggagacatac gaacaagcgc tgcaagcgcc ttcgggtgca acccggcacg
     3121 cggcggatcc acgatcacta cgtcaaatcg ctcaccacga tccgcccagg caggcagaac
     3181 tgcttccgca tctccctgac ggaactgcac atgcgtatat ccgagccgtt ctcgcattgc
     3241 gttcgcgtcc tgtaccgcgg aaggcacctg ctccagaccg acgacctccc gggcacgcgg
     3301 ggccagcgaa atgccgataa gcccgatgcc gcaatacatg tcaagaaccc gctcctcgcc
     3361 cgaaagcgca gccacttcgc tcacgaccga gtacaaccgt tccgcaccgg cgctgttcgt
     3421 ctgaaggaag gagatcggcg ataccgtaac ttccatatcg ccgatcctct ccgaaatcgc
     3481 cggacgaccg gaaagcagaa ccgtcgtctc cgcatgtgcg gtgtcacccc gccgtgtgtt
     3541 gacactgaag agcagacttt tcacctgcgg caattccctg agaagccctt cactcaattc
     3601 atccagaccc gccacctctt ccgtcgcgca caccagattc gccatcactt ccccggttgc
     3661 cgacgcctct cgcagcacca ggtgccgaag tgcgccggcg aacctccgcg ggtcatacgg
     3721 aaccagcgca tttcgccgcg cccactcgca cacccacgcg cgaactgcgt tcatcccttc
     3781 gggcgcaatc cagcagcgct tcacgtcgaa cgtagcatca aatcgcccca cacggtgcaa
     3841 cccgagagtc accgcgccgg tttcatcctt cccgaacgtg aactccatct tgttccgata
     3901 gccgaattcc ggacccgccg caacgacagg caacacttcg atggctgcat cgggcatcgc
     3961 ccgcatgagg gcttccctta ctatccgtcg cttcatctcg tgctgaatgc cgggctccca
     4021 ctgctgccac gtacatcccc cgcactgccc gg
//
LOCUS       DAOSOO010000253         4051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22254_length_4051_cov_6.956957, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000253 DAOSOO010000000
VERSION     DAOSOO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22254_length_4051_cov_6.956957"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..674)
                     /locus_tag="PLT86_13250"
     CDS             complement(39..674)
                     /locus_tag="PLT86_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT37622.1"
                     /translation="MSQPFLPLWHIETDDPVYALTFDDGPHPEITPKLLDVLDRKGVQ
                     ATFFLLGERALEYPGLVAQIAERGHGIGSHGFRHSWNWWRTPDTLARDLDRSERILAG
                     VLREPKLCRPPYGMASPQWLSAARGHGYRTVLWSLGSGDWKYTSPGRIVRRVRRRLRP
                     GAIVLFHECRAETGNGYRHTLEAVSQLLEEESVLRPANLHKLIAKESMPVP"
     gene            complement(671..1681)
                     /locus_tag="PLT86_13255"
     CDS             complement(671..1681)
                     /locus_tag="PLT86_13255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT37623.1"
                     /translation="MPRDKRPILTVSAPPMAESSRSRWLKRLKLAFTIIVFAVLVVSV
                     DPVAIASAWRRIDVRWTALAILLIPHNLFCQYRRISAALMPVYPAISFRTAIHPLLAG
                     LAMGAVTPGRVGELGQVMLLPSGGRTKALGTLTVMRAYGFLSVVALGLAAWMANPALL
                     RTDSRTAVCTGGAVLLVLAALTGVAEWYFRREEHLTTGGLIRKIRGAEGVFAGVKTLS
                     AKDRVRFCGWSLALSFVYLTQLTFLMRAFGAAVPLWDGLLAGAVTTGIVAVLPVAVGN
                     IGVRETAAVVVWKQVGTNAAAAFNGAFLLFLINVVLPGLAGIIWNWWETFRGEGSPSR
                     RP"
     gene            complement(1648..2745)
                     /locus_tag="PLT86_13260"
     CDS             complement(1648..2745)
                     /locus_tag="PLT86_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37624.1"
                     /translation="METCRQLISYIAPGAPATRRPGSGNEPFLRPEIGFTPRWYRQAL
                     GIDFGERWHTDPAYRREAIIAMRGELRRRFPGTAIGGIDRPDEPLDLLTGTYGASTVA
                     AIFGVPIVYSSDNWPNCEHRYLSDDEMDAITPPDLNSNPHFQALMDQVEWIAATEGRV
                     VGYINWQGVLNNAQRLRGEQLFIDMVEEPERCARLFDAVTTTMIDAAKRLHARQSQSG
                     VDVRFFTVSNCLVNMVSSRAYREQLLPFDRRISEAFCCIGIHNCAWNANPYLDPYAEV
                     PRVAYIDMGIMSDLERARRLFPDARRALMYTPMDVADKSLDQIRSDFEHVADTYGPCD
                     IVLADIEAGTPDERVLSLVEICRVISARFSR"
     gene            complement(2779..3486)
                     /locus_tag="PLT86_13265"
     CDS             complement(2779..3486)
                     /locus_tag="PLT86_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT37625.1"
                     /translation="MRVSFLSACVLPGALLFSAGCAGLLERYAIVENSPVVVLYQDAD
                     AHAAQQNYPAALGAYREVIRRYPASAYAEASYLRIASLYLDRAAPERGWSGWEGADYV
                     AARDTLMALLEKFPRTQYGQEARNWIKVLDMALDVDSRTEIVRDTVTVQVPVITADTS
                     DSSAAWQSERARLQRTINAITAERNRFRTQRDTCRAGMERLRAENADLDAQLRRTKSE
                     MERMRRMLVDLERRSGN"
     gene            complement(3506..>4051)
                     /locus_tag="PLT86_13270"
     CDS             complement(3506..>4051)
                     /locus_tag="PLT86_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37626.1"
                     /translation="HEEHLILEATLFSDTLVAADIARICEYDSSGSRCDSIAIAYKAR
                     HQIPEQFRIELAALANLSAPFRLEPLTIYLTDEDGIDYEPRRKIFSTPVTAQRTYLDR
                     EVTRYDPYTSWEYQVYQYTAGYEFQSTGRATLYFDRVNIIGKDLLGPGAKLTLKFRQN
                     RTELGKITWDLDAIRAGSTAR"
BASE COUNT          762 a         1258 c         1290 g          741 t
ORIGIN      
        1 agttgagatg tgccacagaa agctcacgag gacggggttt acggaaccgg catcgattcc
       61 tttgcgatca gtttgtgcag gttcgcgggg cggaggacag actcctcttc caaaagctgc
      121 gagaccgcct ccagcgtgtg gcggtagccg ttcccggttt cagcccggca ttcgtggaac
      181 agcacgatgg caccgggccg caggcgacgc cgcacgcggc ggacgatcct tccgggggag
      241 gtgtatttcc agtccccgga gcccaagctc cagaggacgg tgcggtaccc gtgcccacgt
      301 gcggcggaca gccactgggg actcgccatg ccgtacggcg ggcggcagag cttcggctca
      361 cgcagaacgc cggccagaat gcgctcggat cggtcgagat cgcgcgcaag cgtgtcgggt
      421 gttctccacc agttccacga gtgcctgaac ccgtgcgaac cgatgccgtg cccgcgttcg
      481 gcgatctgtg cgaccagccc cgggtattcc agtgcccgct cgcccaggag gaagaacgtt
      541 gcctgaacgc ctttgcggtc cagcacatcc agcagcttcg gggtgatttc ggggtgcggg
      601 ccatcgtcga acgtaagcgc gtacaccggg tcgtctgttt cgatgtgcca cagcggcagg
      661 aatggctggc tcatggccgc ctcgaagggg agccttcccc gcggaatgtc tcccaccagt
      721 tccagatgat ccccgccagg ccgggaagca cgacgttgat gaggaacagc agaaatgcgc
      781 cgttgaacgc tgccgccgcg ttcgtaccca cctgtttcca gaccaccacc gcggccgttt
      841 ccctcacccc gatgttgccc acggcaacgg gcagcactgc gacgattccc gtggtgacag
      901 cgcccgcaag caggccatcc cacaggggca cggcggcgcc gaacgcgcgc atcaggaacg
      961 tgagctgtgt caggtagacg aaggacagcg caagagacca tccgcagaaa cgcacgcgat
     1021 ccttcgcgga aagtgtcttc acgcccgcga atactccctc cgctcccctg atttttcgga
     1081 tcagcccgcc ggtggtaagg tgttcctcgc ggcggaaata ccattcagca actccggtca
     1141 gcgccgccag aacgagcaga accgcgccgc cggtacacac cgccgtacgc gaatcggtgc
     1201 gcaacagagc ggggttcgcc atccacgcgg cgagaccgag agcaacgacg gaaaggaacc
     1261 cgtaggctct catcacggtc agcgtaccga gggccttcgt gcgtccaccg gaaggcaaca
     1321 gcatgacctg ccccagttca ccgacgcggc ccggcgtgac ggcgcccatc gcaaggcccg
     1381 cgagcagggg gtgaatcgcc gtacggaatg aaatcgccgg gtacaccggc atcagcgccg
     1441 cactgattct gcggtactgg cagaagaggt tatgcggaat gaggaggatc gcgagcgcgg
     1501 tccaacgaac atcgatgcgc cgccacgcag atgcgatggc aacggggtcc acgctgacga
     1561 ccagaaccgc gaacacgatg atcgtgaagg cgagtttcag ccttttcagc catcgcgagc
     1621 gggaggattc cgccatggga ggtgcgctca ccgtgagaat cgggcgctta tcacgcggca
     1681 tatctccacc agggagagga cgcgctcatc cggcgtgccg gcttcgatgt cggcaaggac
     1741 gatatcgcag ggcccgtacg tgtcggcgac gtgctcgaaa tcgctgcgga tctggtccag
     1801 agatttgtcc gcaacgtcca tcggggtgta catgagtgcg cgtcgggcat cagggaacag
     1861 gcgtcgtgcg cgctccagat ccgacatgat gcccatatcg atgtacgcca cccgtggcac
     1921 ctcggcgtac gggtcgaggt acgggttggc gttccacgcg cagttgtgaa taccaatgca
     1981 gcagaacgcc tcggaaattc gtctgtcgaa ggggagcagt tgttcgcgat aggcccggct
     2041 ggaaaccatg ttcacaaggc agttgctgac agtgaagaaa cggacgtcca cgccggattg
     2101 gctctggcgc gcgtgcagac gtttggcggc gtcgatcatt gtggtggtta cggcgtcgaa
     2161 caggcgggcg cagcgttccg gttcctccac catatctatg aacaactgct cgccgcgcag
     2221 gcgctgtgcg ttgttgagca ctccctgcca gttgatgtag cccaccaccc gaccctccgt
     2281 ggcggcgatc cattccacct ggtccataag cgcctgaaaa tgaggattgc tgttgaggtc
     2341 gggcggcgtg atcgcgtcca tctcgtcgtc ggagagatac cggtgttcgc agttgggcca
     2401 gttatcgctc gaatacacaa tcggcacgcc aaaaatcgcc gccacggtgg aagctccgta
     2461 ggttccggta agcaaatcca gaggctcatc gggccgatcg atgccaccga ttgccgtccc
     2521 gggaaaccgg cgccgcaatt cgccgcgcat ggcgatgatg gcttcacgcc ggtatgcggg
     2581 gtcggtatgc cacctttccc cgaaatcgat cccaagtgcc tgtcggtacc accgcggagt
     2641 gaaaccgatt tccgggcgaa ggaagggctc attccctgaa ccgggccgcc gcgtggcggg
     2701 cgctccgggg gcgatgtagc tgatcaactg gcggcacgtt tccataggtg tgacatcccc
     2761 gatgtgagaa atccgcggtc agttgccgga ccggcgttcc agatccacga gcatgcgccg
     2821 catgcgctcc atctcggact tcgttctgcg caactgagcg tccaggtcgg cattttccgc
     2881 gcgcagccgt tccatgcctg ccctgcaggt atcccgctgg gtgcggaagc ggttgcgttc
     2941 cgcggtgatt gcgttgatcg ttcgctggag gcgggcgcgt tcgctctgcc acgccgcaga
     3001 agaatcggac gtatcggcgg tgataacagg aacttgtacg gtaaccgtat ccctcacgat
     3061 ttccgtacgg gaatccacgt ccagcgccat gtcgagcact ttgatccagt tgcgcgcttc
     3121 ctgcccgtac tgggttcgag gaaacttctc cagcaacgcc atcagcgtat cccgtgcggc
     3181 cacgtaatcg gcgccttccc agccgctcca ccctcgctcg ggagcagctc ggtcaaggta
     3241 gagggaggcg atgcggagat acgatgcttc cgcatacgcg ctcgcagggt accggcgaat
     3301 cacctcccgg taggcgccga gcgccgcggg gtagttctgc tgcgccgcgt gtgcgtcggc
     3361 atcctggtat agcaccacca cgggcgagtt ctcaactatg gcgtatcgtt cgagtaatcc
     3421 ggcgcatccg gcgctgaaca gcagggctcc cggcagaacg caggcagaca ggaatgaaac
     3481 acgcatcgtt cgagattcgc tttcctcacc gcgccgtgct gcccgcccgt atggcgtcca
     3541 gatcccacgt gattttcccc agctccgtgc ggttctggcg gaacttgagc gtgagtttcg
     3601 ctccgggccc gaggagatcc ttcccgatga tgttcacgcg gtcgaagtag agcgtcgctc
     3661 gtccggtgga ttggaactcg tacccggcgg tgtactgata cacctggtat tcccacgagg
     3721 tgtacgggtc gtacctcgtc acctcgcggt caagataggt gcgttgcgct gtcaccggcg
     3781 tggagaagat tttccggcgc ggttcgtaat caattccgtc ctcatcggtg aggtaaatcg
     3841 tcaaaggttc gaggcggaag ggtgcggata gattcgccag cgccgccagc tcgatacgaa
     3901 actgctccgg gatctggtgt cgcgctttgt acgcaatggc gatgctgtcg caccggctgc
     3961 ctgaggaatc gtactcgcag atgcgggcga tatccgccgc caccagcgtg tcggagaaca
     4021 gggtggcctc caggatcagg tgctcttcgt g
//
LOCUS       DAOSOO010000254         4049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22277_length_4049_cov_4.713821, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000254 DAOSOO010000000
VERSION     DAOSOO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22277_length_4049_cov_4.713821"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLT86_13275"
     CDS             complement(<1..552)
                     /locus_tag="PLT86_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOT37627.1"
                     /translation="MQQERDLNVVIATDCGSTTTKAILIEKVDGTYRQTFRGEAPTTV
                     EAPFEDVTRGVLNAIQEVEELSGRKILEGEHIITPAEGNRGVDIYISTSSAGGGLQMM
                     VTGVVKSMTGESAQRCALGAGAIVMDVLASNDGRLPHEKIERIRQLRPDMVLQSGGTD
                     GGTVSHVVELAEFIAAADPKHHIG"
     gene            complement(570..935)
                     /locus_tag="PLT86_13280"
     CDS             complement(570..935)
                     /locus_tag="PLT86_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37628.1"
                     /translation="MPPPLELAPADREVLAKVASMLVRRRLGSPALMVLEMSRPINFV
                     ASQFLVFMKPFATALLNPTEYERFVRILEHREGIDALVEAITNGSENGAATAQKRQPS
                     PLASSVGCRDDKTHVESKG"
     gene            complement(1423..2097)
                     /locus_tag="PLT86_13285"
     CDS             complement(1423..2097)
                     /locus_tag="PLT86_13285"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOT37629.1"
                     /translation="MQKENVALDNGTGRHVSVKICGIRTRHDALLAADLGATYVGFVF
                     VRKSPRYIDPAEAAEIVPVLPQGVVPVGVFANQPRDRVLDIISATGVKVAQFSGLESP
                     EYCDSMGRFGVFKAFHVRPPFSFENLEPYHVNMFVLDTYVPGKNGGTGETFDWSLAIP
                     VARKTRVMLAGGLTPANVVEAVETVRPYAVDVSSGVESSPGEKSPHLMETFFQSLHDA
                     GYQTSR"
     gene            complement(2233..2784)
                     /locus_tag="PLT86_13290"
     CDS             complement(2233..2784)
                     /locus_tag="PLT86_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT37630.1"
                     /translation="MSEKAIGLIELSSIASGFQTADAMLKAANVRLLLSRSICSGKYM
                     VLVGGEVGAVQSAVEAGAASADGCLVDRFIIPGVHEDVFAALGRSTIPTIGESLGVLE
                     AFSVASLIEGADAAAKAARVTLVEIRLAMALGGKAFVTMTGDVASVQAAVEAGKARIA
                     EAGMLVNAVVIPRPHEDVYRETV"
     assembly_gap    2807..2906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2907..3901)
                     /locus_tag="PLT86_13295"
     CDS             complement(<2907..3901)
                     /locus_tag="PLT86_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT37631.1"
                     /translation="MALVELVREAGVVGAGGGGFPTHVKLAGKADTVIANGAECEPLL
                     HKDAAVMEREAARVVAGMLATMEAVGATRGIVGIKKKNSRAIEMITAASAGQPISVHL
                     LGDYYPAGDEYDLVYETTGRLIPPAGIPLNVGAVVCNVETFANISRAAENLPVTEKTV
                     TIGGAVRSPVTLTVPVGTPFREVIDAAGGLTTDDPVLCLGGLMMGTTSDNLDTPVLKT
                     TTGAIVLPRTHHVVRRKLKPWNHQAKIGKSACDQCRFCTELCPRYLLGYPVEPHRVMR
                     SLGFTATDRRLLDEWAALCCSCGLCTLYSCPEELFPKEACDTSKDELRKAGFKWTT"
BASE COUNT          799 a         1217 c         1098 g          835 t
ORIGIN      
        1 cccgatatgg tgcttgggat ccgcggccgc gataaactcc gcaagttcca ccacgtgtga
       61 gacggtaccg ccatccgttc cgccgctctg cagcaccata tcggggcgta gctgacggat
      121 ccgttcaatc ttctcgtgag ggagcctgcc gtcgttcgac gcaagtacgt ccatgacgat
      181 ggcgccagca ccaagcgcac accgttgagc actctcaccc gtcatggact tcaccacccc
      241 cgtgaccatc atctgcaatc cgccaccagc cgacgatgtc gagatataga tgtcaacacc
      301 acggttccct tctgccggag tgatgatgtg ttccccttcg agtattttcc ttccgctcag
      361 ctcttccact tcctggatcg cgttcagaac gcctctcgta acgtcttcaa acggggcctc
      421 gacggtagtc ggagcctccc cgcggaatgt ctgccggtag gtgccgtcca ccttctcgat
      481 aaggattgct ttcgtcgtag tgctgccgca gtctgttgcg ataacaacgt tcaggtcccg
      541 ttcctgctgc ataaattcct ttgcgccttt caaccttttg attcgacgtg agttttgtca
      601 tcacggcaac cgacgctcga ggccagcgga gacggttgcc ttttctgcgc ggtcgctgcc
      661 ccgttctcgc tgccgttagt aattgcctcc acgagcgcgt caatgccctc ccggtgttcc
      721 agtatgcgca caaaacgttc gtactccgtc gggttcaaca gcgccgtggc aaagggtttc
      781 atgaacacca ggaactggct tgcgacgaag ttgatcggcc gggacatctc cagaaccatg
      841 agtgcgggag aaccgagtct ccgtctcacg agcatggagg ctactttcgc aaggacttct
      901 ctatccgcgg gagccaactc aagcggggga ggcattgcca gggcatatct gacgctctcg
      961 cggaggcgtt tccaggcgga tgtgtgctct ggcaaaggcg tatacctgct ctcggtttcc
     1021 ggaggtgcga acatacagtc cgcgccacgc atccagatgt ctcggccgcg gaaaagtacc
     1081 caggaaaaca ccgtccctgc gcaacgcgac acgtttgaag cgcgcccagg ggtggaatcg
     1141 agtcaccccg agatgtcggg catgcacgcc gcgcggattg atttcgtaaa aggtaggtaa
     1201 aaaataccga gaaaggctca gaaaaagaaa cccgaaggct ccgaggccga tacccgggct
     1261 tgtgaacccg aaaccggcga ccgctgatgc cgcgaccatg gcgcttacga gtgaccatcg
     1321 ccggaacggg gcatcctcgc gccacggatg agagaaaaac gtgattgcgt cttctggagg
     1381 tgacgcagga ggatgggcgt tgacaggcat cacggccggc tgtcagcgac tggtctggta
     1441 gcccgcgtca tgcaggctct ggaagaacgt ttccatgagg tgaggacttt tttcgcccgg
     1501 actggattcg acgccgctgc tcacgtccac cgcgtaaggg cggacggttt ctaccgcctc
     1561 gacaacgttt gccggagtga gtccgcccgc cagcatcaca cgtgtcttcc tggcgactgg
     1621 gatcgcgaga gaccagtcaa aagtttcgcc cgtccctcca tttttgcccg gaacgtacgt
     1681 gtcgagaacg aacatgttga cgtggtacgg ttccaggttc tcaaaggaaa acggcggccg
     1741 aacgtgaaac gctttgaaca ccccgaatct gcccatgctg tcacagtact ccggggactc
     1801 cagaccgctg aattgcgcta ctttgacacc cgtcgccgat attatgtcga gcaccctgtc
     1861 tctcggttga ttggcaaaaa cccctaccgg aacaacaccc tggggtaata ctggcacgat
     1921 ttctgcggct tctgccgggt cgatgtagcg cggacttttc ctgacaaaca caaaccccac
     1981 ataggtggcg ccgagatcag cggcgagcaa tgcatcgtga cgggtgcgga tcccgcagat
     2041 tttcactgag acgtgcctgc ctgtcccgtt gtccagggcg acattctcct tctgcatact
     2101 ctgctgctca ctttcccgat cgtgtgcctt tggaaatgaa acgcgctatc gtattctctc
     2161 gcaaatagat aaatgcgcag acttgggctg cggtacccga gagggtagag atgctctcca
     2221 agaacgactt cgtcacacgg tttcgcggta gacgtcctcg tggggccgcg ggataaccac
     2281 tgcgttcacc agcatgcctg cttcggcgat gcgagccttg cccgcttcca ccgccgcctg
     2341 cacggaggcg acatcacccg tcatagtgac aaaggctttc cctcccagcg ccatggccaa
     2401 cctgatttcc acaagggtaa ctctcgcagc ctttgcggca gcgtcggccc cctcgatcag
     2461 cgaagcgacg ctgaacgcct cgagaactcc aagcgattcg ccaatcgtcg ggatcgtcga
     2521 gcggcccagc gccgcaaaca cgtcttcgtg cacgccggga ataatgaacc tgtccaccag
     2581 acagccatcg gcagacgccg cccccgcttc taccgcgctt tgcactgccc ccacttcgcc
     2641 cccgacaaga accatgtatt tacccgaaca aatgctgcgg gaaagcagga ggcgcacatt
     2701 tgcggccttc agcatcgcgt ccgccgtttg aaagccggac gcgatactgg agagctctat
     2761 caacccgatc gctttctcac tcattttgtc ctctgaatgg caatacnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnngtgg tccatttgaa accggctttc cgcagttcgt
     2941 ccttggacgt atcacacgcc tctttcggga aaagctcttc gggacaggaa tacagcgtgc
     3001 aaagtccgca actgcaacac agggcggccc actcgtcgag aagtcttctg tcagttgcgg
     3061 taaacccgag actgcgcatc acgcggtgag gctccacggg gtagcccagc aggtaccgcg
     3121 ggcacaattc cgtgcagaag cggcactgat cgcaggctga cttcccgatt ttcgcctgat
     3181 ggttccaagg tttgagtttg cgccggacga cgtgatgggt gcgcgggagg acgatggcgc
     3241 cggtcgttgt tttcagcaca ggagtgtcga gattgtccga tgttgttccc atcatgagcc
     3301 cacccaggca caggacagga tcatccgtgg tcagcccccc cgccgcgtct atcacctcac
     3361 ggaacggcgt tcccacaggc accgttagag ttacgggaga gcgcaccgcg cccccgatgg
     3421 tgacggtttt ttccgtgacc ggcaggttct ctgctgctct cgagatgttc gcgaatgttt
     3481 ccacgttgca caccaccgca cccacgttaa gcgggatgcc ggcaggcgga atgaggcgtc
     3541 ccgttgtttc gtacacgaga tcgtactcat cgcccgcggg ataataatcg ccgagaaggt
     3601 ggactgaaat cggttgcccg gcgctcgcgg cggtgatcat ttctattgcc cgtgagtttt
     3661 tcttcttgat gcctacgata ccacgcgttg ccccgacggc ctccatagtg gccagcattc
     3721 cggcgacgac gcgggcggcc tctcgttcca ttactgctgc gtctttgtgg aggagcggct
     3781 cgcactcagc tccgttggcg atgacggtat cagctttccc tgccagcttc acgtgagtcg
     3841 gaaatccgcc gccgcctgct cccaccacgc ccgcttcgcg gacgagttcc accaatgcca
     3901 tgttttcatc cctgtggttt gcgatctcaa gttgctaatt tgaaagagcg aaaagtggca
     3961 gacaatgcca aggggtagcc cgaaacacgc gatcggtgga gacggagcag caacaatgat
     4021 agacgttcat atgcatatcg ggaagctct
//
LOCUS       DAOSOO010000255         4041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22338_length_4041_cov_5.306071, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000255 DAOSOO010000000
VERSION     DAOSOO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22338_length_4041_cov_5.306071"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..711
                     /locus_tag="PLT86_13300"
     CDS             1..711
                     /locus_tag="PLT86_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOT37632.1"
                     /translation="MTLRLCFKHFRDVRAVLLALSLCSLLNGYPREAHAADSFAGAAI
                     AQLQYGGGGDWYANPSSLPNLLKFARKEAGISVSPTPGRVRITDPELFAYPLIYMTGH
                     GNVRFSDEEVVRLRRYLLSGGFLHADDNYGLDESFRREMRRVFPDNALVELPFDHPIY
                     RSWFVFPNGLPKIHEHDGKPAQGFGIFAGGRLVVFYSYESDLGDGWEDQHVHNDPEEK
                     RLEALRMGTNILVYALTH"
     gene            complement(741..1991)
                     /locus_tag="PLT86_13305"
     CDS             complement(741..1991)
                     /locus_tag="PLT86_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37633.1"
                     /translation="MRTARIRPDLLGPECPEGLEIVQITTGELPSTHIYMEAQIFTPD
                     SKRFLLHESATPHGSDPKDPNHRYLLCDLEQGCKLSPVTQETGATAPSVSPDGMHVYY
                     FVDETRLDGGKLTLKRMNLDGSAREVLYVLQGTVPGTPFFPSQIYPLSTIRSDGKSLA
                     LSCFLADVGSGTRTWGLMVFDLENPSVTLILEGPSWCNVHPQYSRSIHPGSQRDILVQ
                     ENHGNTHTPEGEILDLTGGNGADIHVIRDDGSSFRNMPWGRDGNEFCQGHQCWRGNTA
                     WAITSTDTRHPDEQQLIESVCVPHTGHDGARSPGAIRNNLSREFPSPSFSHFATDASG
                     SWFVTDTFACDNGGRVFVARMGNAGTDPLSSWTCIAYPRCSWRKDAHIHPFLSPDGKT
                     AFFNSDESGVLQAYMVRGLESLGI"
     gene            2037..2411
                     /locus_tag="PLT86_13310"
     CDS             2037..2411
                     /locus_tag="PLT86_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37634.1"
                     /translation="MAIIASVYQLVSAVRQDNVNTVASELERLLKVIRIEQVADQTGI
                     MCFTEMFLCRHATELNSRTTVLDRHADLLPRPILGNKTGTWNFRGKLHARREHGFGRS
                     SQIRGLNDLASANLICNKADQA"
     gene            2433..2696
                     /locus_tag="PLT86_13315"
     CDS             2433..2696
                     /locus_tag="PLT86_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37635.1"
                     /translation="MDVDTDPSTGKVENLLNPGHTNSPPRFTVPGTEIELAQFSPFHF
                     GYGSRRVRGAVHSFVVADHGYIVAAQPNVKFDFVRTDPDRCAK"
     gene            complement(2767..3147)
                     /locus_tag="PLT86_13320"
     CDS             complement(2767..3147)
                     /locus_tag="PLT86_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37636.1"
                     /translation="MVTITTDGVTVRIKVRGLHQLWAFRRTLEIPLRHVTSVTRMAPG
                     LRPPWLRLIGTAIPGLIYAGMFRGRGRREFWDWTCGKDAVQLDLAEETLSRIVVSVDN
                     AEEVIRQISGEFRAGDSKMDENIA"
     gene            complement(3205..>4041)
                     /locus_tag="PLT86_13325"
     CDS             complement(3205..>4041)
                     /locus_tag="PLT86_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37637.1"
                     /translation="DRTTLLFDPELKAPFVGFTRRHGKDMQAESGRRVIYRIESEDFA
                     HWSEPTPLLVPDLADSWDLQFYGMPVFRHRDLYLGGLKRLWSTPDRIDTELVWSRDTK
                     SWGRSRVPFLDNGPSDSWDSSWVGLASSPPLEIGGQLFFYFEGRRQAHGQRFPFPQGS
                     IGLAVLPKDRFAAIEAGPAEGYVETKPFKWHGGRLGVNLRTNGHGYLRVALLDEDGSE
                     IPGFSAPVCTSLTGDIPCAEPSWGNQPPRELIGHSVRLRIHMVNTRLYAVYALAAPND
                     HA"
BASE COUNT          825 a         1218 c         1122 g          876 t
ORIGIN      
        1 atgacgttgc ggctctgttt caaacacttt cgcgatgttc gcgcagtact gctggcgctt
       61 tccctgtgtt ctctcctgaa tggatacccc cgggaagcac acgcggcaga ttctttcgcc
      121 ggggccgcta ttgcgcagtt gcagtacggc ggcggcggtg actggtacgc gaatccttcc
      181 tcgctcccta acttgctgaa gttcgcccga aaagaagccg gaatatccgt ttcccccaca
      241 ccgggacgag ttcgcataac agacccggag ctgtttgcct atcccctcat ctacatgacc
      301 gggcacggca acgttcgctt cagcgacgag gaagtggtcc ggcttcgccg atatctcctt
      361 tcaggaggtt tccttcacgc ggatgacaac tacggtcttg atgagagctt ccggcgggaa
      421 atgagacggg tgttccctga caacgcactc gtggaactgc cttttgacca tcctatctac
      481 cgaagctggt tcgtgtttcc gaacggattg cccaaaatac acgagcacga cggcaaaccc
      541 gcgcaaggat tcggcatttt cgccggcggc cgccttgttg tgttctactc atacgagtcc
      601 gatctgggtg atggatggga ggatcagcac gtgcacaacg acccggagga gaaacggttg
      661 gaggccctcc gaatgggaac gaacatcctc gtgtacgcgc tcacgcattg accgcgcccg
      721 gtgattccct gccggccaca tcaaattccg agtgattcca gcccgcgaac catgtaagcc
      781 tgcaaaacac ccgattcatc agaattgaaa aacgcggttt tgccgtcggg cgacaggaac
      841 gggtggatat gggcgtcctt cctccacgag caacggggat aggcgataca cgtccatgaa
      901 gagagggggt ccgttcctgc gttccccatt ctggcgacga aaacccgtcc tccgttatcg
      961 cacgcaaatg tgtcggtaac gaaccatgag ccgctggcgt cggttgcgaa atgggaaaac
     1021 gaaggcgagg ggaactcccg gctgaggttg ttcctgattg cccccggtga acgcgcgccg
     1081 tcgtggcccg tgtgtggcac acaaacgctc tcgataagct gttgctcgtc cggatggcgc
     1141 gtatctgtgc tcgtgatcgc ccaggcggtg tttccccgcc agcactggtg cccctggcag
     1201 aactcgttcc catcccgtcc ccacggcatg ttacggaagc tcgatccgtc atctcggatc
     1261 acgtgtatgt ccgcgccgtt tcctcccgta aggtcaagga tttccccttc gggggtgtgg
     1321 gtgttaccgt ggttctcctg aaccagaatg tcgcgctgag acccgggatg aatcgatcgg
     1381 ctgtactggg gatgtacgtt gcaccagctg ggtccttcca gaatcagagt cacggaaggg
     1441 ttttccagat cgaataccat cagcccccac gtccttgtcc cggaacccac gtcagccagg
     1501 aagcaggaaa gggcgaggct tttcccgtcc gaacgaattg tcgacagggg ataaatctgg
     1561 ctgggaaaga acggcgtacc tggtacggtt ccttgcaaca cgtagagcac ttcccgcgca
     1621 cttccgtcga gattcattcg tttcagcgtc aatttcccac cgtcgaggcg ggtttcgtcg
     1681 acgaaatagt acacgtgcat gccgtctggc gatactgaag gcgctgttgc ccccgtttcc
     1741 tgtgttaccg gcgacagctt gcaaccttgc tcaagatcgc agaggaggta ccggtggttc
     1801 ggatccttcg ggtcactgcc atgtggcgtt gcagattcgt gaaggagaaa gcgttttgaa
     1861 tcaggagtaa atatctgcgc ctccatgtag atgtgggtgg agggcagctc accggtggtg
     1921 atctggacga tttccaaccc ttccgggcat tcggggccga gcaggtccgg cctgatcctg
     1981 gcagttctca cgtgatcccc attccttgcg tgtagacggg atttggttca cgccgcgtgg
     2041 ctattatcgc gagcgtgtat caactggtga gcgcagttcg tcaggataac gtcaacaccg
     2101 tggcgtccga actcgaacgc ctcctcaaag tcatccgaat agaacaggtt gcagaccaaa
     2161 ccggcatcat gtgcttcacg gaaatgttcc tgtgtcgcca cgcgacggaa ctgaattcgc
     2221 gcacaaccgt acttgatcgc cacgcggatc tgctcccgag gcccattctg ggaaacaaga
     2281 caggcacgtg gaatttccgg ggcaaactcc acgcacgcag agagcacggc ttcggtcgct
     2341 ccagccagat acgcggactc aacgatctgg cgtctgcgaa tctcatttgc aacaaggcgg
     2401 accaggcgtg aatcattccc cggttctttg atatggacgt tgataccgac ccgtccaccg
     2461 gcaaggtcga gaacctcctc aatccggggc acacgaactc cccgccacgc ttcaccgtac
     2521 cgggcaccga aatcgagctt gcgcagttca gcccattcca tttcggatat ggctcccggc
     2581 gcgtccgcgg tgcggtccac tcgttcgtcg tggcagacca cggctacatc gtcgcggctc
     2641 aaccgaacgt caaattcgat ttcgtgcgca ccgaccccga tcgctgcgcc aaatgaaggg
     2701 agagtgttct ctggacacgc cttgctcagt ccacggtgcg cgcagacacg cggaaaagta
     2761 tcattattac gcaatgtttt cgtccatttt gctatcacct gcgcggaatt ctccgcttat
     2821 ctgccgtatc acctcctctg cgttgtctac gctcaccact attcggctga gcgtttcctc
     2881 cgcgaggtcc agttgcaccg catccttgcc acaggtccaa tcccagaact ccctcctgcc
     2941 acgcccgcga aacattcccg cataaatgag cccgggtata gcggttccta tgaggcgtaa
     3001 ccagggtggg cggagcccag gagccatccg cgttacagat gtcacatgcc tcaacggtat
     3061 ctccagtgtt cgcctgaaag cccagagttg gtggagcccc cggactttga tcctgacagt
     3121 gactccatct gttgtgatgg taaccatagc gtctgtcctc ttccggaact caatcctcgg
     3181 ccatcaccga ccgatctctg ttcctcacgc gtgatcgttg ggtgcggcaa gcgcgtatac
     3241 tgcgtacaga cgagtgttca ccatgtggat tcggagacga acgctatgtc cgatgagttc
     3301 ccgaggtggt tggttccccc acgatggctc ggcgcaggga atgtccccgg taaggcttgt
     3361 gcagacaggg gcggagaatc ccggtatctc actgccgtcc tcgtccagta acgcgacacg
     3421 caggtacccg tgcccattgg tgcgcaggtt gacccccaat ctgcctccgt gccatttgaa
     3481 cggtttcgtc tccacgtatc cttccgccgg accggcttca attgctgcga atctatcttt
     3541 cgggagcacc gcgaggccta tcgaaccctg tgggaacgga aacctttgcc cgtgcgcctg
     3601 acgcctgcct tcgaagtaga agaaaagctg accaccgatt tccaacggcg gagacgaggc
     3661 cagacctacc cacgaggaat cccaggagtc acttggcccg ttatccagga aaggcacacg
     3721 ggatcttccc cacgatttgg tgtctctgct ccacacaagt tccgtgtcaa ttcgatccgg
     3781 tgtcgaccac agacgtttga ggccgccgag gtacaggtcg cggtgtcgaa ataccggcat
     3841 cccgtagaac tggaggtccc acgaatccgc aaggtcggga accagcaaag gagttggttc
     3901 cgaccagtgt gcaaaatcct cactttcaat ccggtaaatg acgcggcgac cggattcagc
     3961 ctgcatgtcc tttccatggc ggcgcgtgaa gcccacgaag ggagccttca gttccgggtc
     4021 aaaaagcaaa gtcgtcctgt c
//
LOCUS       DAOSOO010000256         4038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22361_length_4038_cov_5.283455, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000256 DAOSOO010000000
VERSION     DAOSOO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22361_length_4038_cov_5.283455"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..543
                     /locus_tag="PLT86_13330"
     CDS             217..543
                     /locus_tag="PLT86_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT37638.1"
                     /translation="MAAIRTITPAQLEEWLENGKDFVLVDVREQNEWEICHIKGALFR
                     PLSQISSWIDALDPNKEYVFHCHHGGRSHQACALAIARGVENVISLEGGIDSWCTAVE
                     PQMKRY"
     gene            581..1891
                     /locus_tag="PLT86_13335"
     CDS             581..1891
                     /locus_tag="PLT86_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT37639.1"
                     /translation="MRTALAQITGDDFVTEVPAEFASLDGVRPRYLVKPGNPQEIAAV
                     LEAAGREQWAVLPIGSGSAIAIGNPPRRGDIWLSTARLNAFEEYESADLTATVQSGCL
                     LFDLNRVFGEHGQILPLDPPGAGSRTIGGIVATAQTGTLRLGYGQPRDWILGLQIATP
                     DGKLIRSGGKVVKNVAGYDLTRLFTGSYGTLGIITQVSLKVYPKPATEMTMVCGSDEI
                     VRVWKTVEHMLSGNINPVSLEIVSENALRRVGLEGELPGYCLCVRVAGEEADVRVQTM
                     QMERIASESELSVRAPVPAETAGAFWQMVSDLPLGFPIVVRLNTVPSRLPRFLSMAAD
                     TLRQAAECVYFSASAGIGCATLMVEGTSDATLMNKLLPALSEIRRQVVSSGGSVILQK
                     LPTGWKEHIDVWGPTGAAASLMKGMKQAFDPSGVLNPGRFVEGI"
     gene            1910..3220
                     /locus_tag="PLT86_13340"
     CDS             1910..3220
                     /locus_tag="PLT86_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOT37640.1"
                     /translation="MFGKRKRLLPDAVKEQENNLLACVHCGFCLPHCPTYSVLGDEND
                     SPRGRLYLMRAVAEGRITPDDAYTRHIESCIVCRACETACPSGVRYGTIMEAARCDLR
                     RLGASDMGWLARVLRWMGLYLLPRRSLLRPAVFPMRIIRHLRPTDGWWRWLPSIVRLG
                     LEMLPPPPLKRKFAPVSSHAPDKGVVVQFRGCVMDELFKNVNQATTRVLEVNGFRVTV
                     PQAQTCCGSLHDHSGFVDAARELARKNIDSLEDGNDTPVVVNAAGCAALMKEYGELLK
                     EDSRYAARAHRFASRVCDVTELLARTGVVKGASIQRKVTYDAPCHLYHAQKVKTAPLD
                     VLKAVPDLDFRPLRGADRCCASGGIYNLTHPDIAEEVLQRKLSALFETGAETLVTANP
                     GCQMQIQQGVRTAGMDVSVAHVIELLDESYEKAGYYDSTTSLKG"
     gene            3223..>4038
                     /locus_tag="PLT86_13345"
     CDS             3223..>4038
                     /locus_tag="PLT86_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOT37641.1"
                     /translation="MLITDVDAFVLAMPHVTDAADGTQDTFLVRIRTNTGVTGWGESD
                     ASPLVSLAAYYCPMSHGNIINIRESLLGERVDTPEDIRRLHQKVRRQGLDIEQIDHAY
                     SAADIALWDALGKQLDEPVYHLLGSPIAYPKLPYASVLFADTPQETLERARGLRKLGF
                     RAAKFGWGPMGKRGADFDVDLVAAAREGMGEEAEIMIDAGVAWGTDYETAYARALAFS
                     PFRPTWLEEPLLPDAVDEYGKLKALNPPVPIAAGEGAARFRTAQDILCNGKVDF"
BASE COUNT          878 a         1125 c         1212 g          823 t
ORIGIN      
        1 tttgctgttt ccatagagca agctcctgca cgtgaggcgg attactgaat cgaacaaaaa
       61 ctcggtgtac tgactcgagc cgatgctcag aatgtaccga ggacaccaga ggtgtggcaa
      121 acaaccccgc gcggggtctc gtcgcctagg aatccccgtt gaacgggcgt atctttggtt
      181 attattttcg actgaaaatt caggacgagg aaaggcatgg cagccatcag gacaattacg
      241 cccgctcaat tggaggaatg gctcgaaaac ggaaaggact tcgttctcgt agacgtccgc
      301 gagcagaacg agtgggagat ttgccacatc aaaggagcgc tcttccgccc tttgagccag
      361 atttcgagtt ggatagacgc tcttgacccc aacaaggagt acgtcttcca ttgccaccac
      421 ggaggacgta gtcatcaggc ctgcgccctg gcaatcgcgc gcggtgttga aaacgtcatc
      481 agcctggagg gtggcatcga cagctggtgc acggctgtcg aaccacaaat gaaacgctac
      541 tgacgacact gacggcatca ctggattgac ggtaccggga atgagaactg ctcttgcgca
      601 gataaccggc gacgattttg tcacggaagt gccggccgaa ttcgcatcac tggatggcgt
      661 tcggcccaga tacctcgtca aaccgggcaa tcctcaagaa atcgctgccg tgctggaagc
      721 tgccgggcgg gaacagtggg ctgtgcttcc catcggctcc ggctctgcaa tcgccatcgg
      781 caacccaccc cgcagaggag acatctggct ttccactgcc cgcctcaacg cgttcgaaga
      841 gtacgagtca gcggatctca ccgccactgt tcagagcgga tgcctcctct tcgacctgaa
      901 cagggtattt ggcgagcacg gtcagatcct gcctctcgat ccccccggtg cgggcagccg
      961 cacaatcgga ggaatagttg ccaccgcaca gacagggacg ctgcgccttg gttacggcca
     1021 accgcgggat tggatccttg gcctccagat cgccacgccg gatgggaagc tcatccgaag
     1081 cggcgggaag gttgtcaaga atgttgcggg ctacgatctc actcgtctct tcaccggttc
     1141 ttacggcacg ctgggaatca tcactcaggt cagtctgaag gtttacccga aacctgccac
     1201 agaaatgaca atggtgtgtg ggtcagacga gatcgttcgg gtgtggaaga cggtggaaca
     1261 catgttgtcc gggaacatca atccggtttc actggaaatc gtgtcggaaa acgcgctcag
     1321 acgggtgggt ttggaaggag aactgccagg ttattgcctc tgtgtccgtg tcgcaggcga
     1381 agaggccgat gtgcgtgtgc aaacgatgca gatggaaaga atcgcctcgg aatcggaatt
     1441 gagcgtgcgt gcacctgtcc ccgcggaaac agcaggcgct ttctggcaga tggtctctga
     1501 ccttcctttg ggatttccga ttgttgttcg tctgaacact gtcccgtcgc ggctcccgcg
     1561 ttttctctcg atggctgccg acacgctgcg acaggccgcc gaatgcgtat acttctcggc
     1621 atccgccggc atcgggtgcg cgacgctgat ggtagaaggc accagcgatg cgaccctgat
     1681 gaacaagctt ctcccggcgc tttccgaaat ccgccgccag gtggtgtcgt cgggcggttc
     1741 ggtcatcctg cagaagttgc caaccggatg gaaagagcac atcgacgtgt gggggccgac
     1801 cggtgcggcc gcgtccctga tgaagggaat gaaacaagcc tttgatcctt ccggggtgct
     1861 caatcccggc aggttcgtgg aaggtatctg aagcagtctg gaagtaaccg tgtttggaaa
     1921 acgaaaacgt ctgctgccgg atgcagtaaa agaacaggag aacaatctcc tcgcgtgcgt
     1981 tcattgcggc ttctgtctcc cccattgtcc cacgtacagc gtcctcggag acgaaaacga
     2041 ttctccccgc ggaaggctct acctcatgcg ggccgtggcc gagggcagaa tcacacccga
     2101 tgacgcatac acccgccaca tcgaatcctg catcgtctgc cgcgcgtgcg agacagcgtg
     2161 cccttccggc gtgcgctacg gaaccataat ggaggcagcg cggtgtgatc tgcgtcggtt
     2221 gggcgcatcg gacatggggt ggcttgcgcg tgtgttacgg tggatgggtc tttacctcct
     2281 gccccgccgt tctctgctca gaccggcggt attcccgatg cgtatcatcc ggcatctgcg
     2341 accgaccgac ggctggtggc ggtggttgcc atcaatcgtc cggctggggc tcgaaatgct
     2401 gcctcccccg ccgctgaaac ggaaattcgc gcccgtttca tcgcatgccc ctgacaaagg
     2461 ggttgtcgtg cagttccgcg ggtgcgtgat ggatgagctg ttcaaaaacg tgaatcaggc
     2521 taccacacgc gtccttgagg tgaacggttt tcgggtcacg gttcctcagg cacagacctg
     2581 ttgcggttcg ctccatgacc acagcggatt tgtagacgct gccagggaat tggccaggaa
     2641 aaacatcgat tctttggagg atggcaacga cactcccgtc gtcgtcaacg cggcaggctg
     2701 cgcggctttg atgaaggaat acggggaatt gctgaaagaa gactcccgct acgctgcgcg
     2761 tgcccataga tttgcatctc gcgtatgcga tgtgactgag ttgctcgccc gaaccggggt
     2821 tgtcaagggc gcatctatcc agcgaaaagt cacatacgac gcaccatgcc acctgtacca
     2881 cgcccagaaa gtgaaaactg ccccactcga cgttctcaaa gcggttccag acctcgattt
     2941 cagaccgttg cgcggagccg atcgatgttg tgcgtccgga ggcatataca acctcacgca
     3001 tcccgacata gccgaagaag tgttgcagcg caaattgagc gcgttgttcg aaacgggcgc
     3061 ggaaaccttg gtgacggcta atcctggatg tcagatgcag attcagcaag gcgtccgtac
     3121 ggcaggtatg gacgtttccg ttgctcacgt aatcgaactt ctggacgagt cgtacgagaa
     3181 agccggttat tacgacagta cgaccagttt gaaaggttaa cgatgctgat tacggatgtt
     3241 gatgcgtttg tcctcgcaat gccccatgtt accgacgcgg ctgacggcac gcaggatact
     3301 ttcctggtgc gcattcgaac aaacaccggg gtgacgggtt ggggagaatc cgacgcgtcg
     3361 ccgttggtga gccttgccgc atactactgc ccgatgtcac acggaaatat catcaacatc
     3421 agggaatcct tgctcgggga gcgtgtcgac acgccggaag atatcagaag gctgcatcag
     3481 aaggtccgca ggcagggact ggatatcgaa cagatcgatc acgcgtacag cgctgccgat
     3541 atcgccctgt gggacgcgct gggaaagcaa ctggatgagc cggtgtatca cctcctcggg
     3601 agtccaatcg cgtatccaaa gctcccctac gcgtctgttt tgttcgcaga tacgccgcag
     3661 gagacccttg aacgagccag ggggctgaga aaactgggct tccgtgccgc gaaattcggt
     3721 tggggaccga tggggaaacg cggagcggat ttcgacgtag acctggttgc cgccgcgcgc
     3781 gagggtatgg gtgaagaagc ggaaatcatg atcgacgcag gagtcgcctg gggaactgac
     3841 tatgaaaccg catatgccag agctctcgcg ttctcaccat tccggcctac ctggctcgag
     3901 gagcctctcc tgccagacgc ggtagacgaa tacggcaagt tgaaagcgct gaatccgccg
     3961 gtgcccatag ctgcgggaga aggcgcagca cgcttcagaa cggctcagga tatcctttgc
     4021 aacggaaaag tggacttc
//
LOCUS       DAOSOO010000257         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22417_length_4032_cov_4.103093, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000257 DAOSOO010000000
VERSION     DAOSOO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22417_length_4032_cov_4.103093"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLT86_13350"
     CDS             <1..183
                     /locus_tag="PLT86_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37642.1"
                     /translation="LRDAALRELMGYRWDRLHRDMGERRRLIMEEGRKRGLPMLDVAP
                     EDWRGVWSETAPRYIG"
     gene            679..1047
                     /locus_tag="PLT86_13355"
     CDS             679..1047
                     /locus_tag="PLT86_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37643.1"
                     /translation="MPDHIRNLTIMQTDTAVVLSALGGPEVTVITDGRRRDVVWLDGN
                     NAKVKAEWTDNGLVIERREESGPTVRETYLRYPRSTRLSVEMEIKGSNLPRAMKLVRL
                     YDLLAAPEDTSHSILGDPMW"
     gene            1261..1845
                     /locus_tag="PLT86_13360"
     CDS             1261..1845
                     /locus_tag="PLT86_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT37644.1"
                     /translation="MAGTVKVMLLGDSIRMSYQPLVARLLEGRAEVLGPADNCQYSLY
                     TLSSLARWTNELGKPDIVHWNNGLHDAGHNPQRHPIQIPIDMYRANLAHILDALSRLT
                     DKIIWATTTPVHPERPFRADAWSWRNEEIDRYNRAALELMEERGIPVNDLHAVVWSNT
                     SEYLGPDQLHLSEAGKQACAEAVAESVSRLLLAS"
     gene            complement(1892..3346)
                     /locus_tag="PLT86_13365"
     CDS             complement(1892..3346)
                     /locus_tag="PLT86_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37645.1"
                     /translation="MRTARNPNLLFIMTDQQRRDALSCMGSRFVHTPSLDRLAVEGVR
                     FTNAITPSPICVPARASVLTGLPIHHTGCITNPDRLRTPASDLTTFDDALTAHGYTAE
                     YIGKWHAPEALLRPYSRLPTSVSLDTFYRGYLAEKGYGSPHQGASHAYISPLSGQPYD
                     PDPMDHVYRSLNLGRGHTRPEPGLPFGRDSVRAEHSISAMIADETMRSIERLKDVPFS
                     ITSSFLFPHDPLIVPRPYCDSVRAADMATPETFDDARMNTPYKDFVWQMDDLERRHLH
                     LLMARYYAAVQEVDHHIGRILDALDTSGLTDNTLVVFTSDHGEMLGDHGLMTKFVHYR
                     EAVGVPLLMRMPGRIRVGSTVHAPVCLTDLFATISDYLGVTPRDRRGRSLQSWIAGDA
                     PEPDPIVFSQFEHWNVMAQTCEWKYVWSNHADEVDMLFDLTRDPEEVNNLLGANQDRA
                     RYLQQAFAMKERLVGWMEETKHPWLERVAATPVR"
     gene            3524..4012
                     /locus_tag="PLT86_13370"
     CDS             3524..4012
                     /locus_tag="PLT86_13370"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOT37646.1"
                     /translation="MGAGSFVRRRVISPIMDLLKQGVTPEKIALSMAVGGALGVFPVL
                     GSTTILCTLAAFLFRLNMPAIQIVNYVMFPLQLLLLIPFIRFGEWIFHAAPIPFSASE
                     MAAMFRSDFQGSMGLLWVTGLQAIVAWCAFMPPAAVLVYLVLAPGLRRIQARFRRTDG
                     PS"
BASE COUNT          788 a         1150 c         1267 g          827 t
ORIGIN      
        1 ttgcgtgatg ctgccctccg ggagctcatg ggctaccgct gggacaggtt gcacagggac
       61 atgggcgaaa gacgacgtct cataatggag gaaggccgga agcgcggcct gccgatgctg
      121 gatgttgctc cggaagactg gcgcggggta tggtcggaaa ccgccccgcg gtatatcggt
      181 tgaccgggaa acaggatgtc gtcccatccg tcggggtttc ctgtttgagt tccgaacagc
      241 gcagtcatga aagcgtggct gtctcgcagc aaacagccct tcgggcagat accaaaacaa
      301 ccggcgcatt cgttcagagg tacagatcat tcctgttcga ccactcagga tggaggtgaa
      361 cgaatgcgtt gctcctcttt acatgcgctc cgagcgggcg ccatcgtgat tgtctgtttc
      421 cttacgtgga cttcgtcgcg cgcttcaacc cgcacgcccc cgatcgacat tgatctgtcc
      481 ggtcgctggg tgttcagtga aactgcgtcg cgggagcaga atcaaagtgc gcgagggggc
      541 gacgctcgtg gaatgcccgg cagccagaga aggccggcgc gcggagtgcc gggcggtacg
      601 gatctgggag gtggttttcc gggcggcagg ccgcgtgatg gaagcgcccc gggtggcgcg
      661 cgtcctgagc gcccccgcat gcccgaccac attcgaaacc tgaccatcat gcagacggac
      721 acggccgttg tgttgtctgc actcggcggg cctgaggtta cggtgattac cgatgggcgc
      781 agacgcgacg tggtgtggct tgacgggaac aacgccaaag tcaaagcgga gtggacggac
      841 aacggcctcg tcatagagcg cagggaggaa tccggtccga ccgtgcggga gacgtacctg
      901 cggtacccgc gctcgacgcg cctgtccgtg gagatggaaa tcaaagggag caacctgccc
      961 cgcgcgatga aactggtacg gttgtatgat ttgctggccg ctcctgagga tacctcccac
     1021 agcattctcg gtgatccgat gtggtgaagt gataccgcgc ggcgcagacg cgtcttacag
     1081 atctcgcacc gtcacgtttc gtcagacgtg aacgaggtgg tttggaaaca tcaggaatgg
     1141 ggcaccggtc gccagccggg aggctgcaat gccggtagaa cacgccagct ctgatgcggg
     1201 tactcggggc ggtttgatgc tgattttgag aacacatcgg cgccacggaa agggacaacg
     1261 atggcaggga cggtgaaagt aatgctgctg ggagattcaa tccgcatgag ctaccagcca
     1321 ctggtggcgc ggctgctgga aggcagggcc gaggtcctgg gccccgcgga caactgccag
     1381 tacagccttt acacgctttc atccctcgca cgttggacca acgagctggg gaagcccgac
     1441 atcgtgcact ggaacaatgg tctccatgac gcaggccaca atccgcaacg gcacccaatc
     1501 cagatcccga tcgacatgta ccgggcgaat ctggcgcaca ttcttgatgc gctgagccgg
     1561 ctgacggaca aaatcatatg ggcgacgacg acaccggtgc accctgaacg cccgtttcgc
     1621 gccgatgcgt ggtcctggcg aaacgaggag atcgatcggt acaatcgtgc ggcccttgaa
     1681 ctgatggagg aaagggggat cccggtcaac gatctgcacg ccgtggtgtg gagcaacact
     1741 tcagagtatc ttggccctga tcagcttcac ctttccgagg caggaaaaca agcctgcgcg
     1801 gaggcagtcg cggagagcgt gtcccgtttg ctgttggcat cctgacggca tcacgtcggt
     1861 tcgtgggtct caatccctgt ctcacgagcg tttatctcac cggtgtggcc gccacccgct
     1921 ccagccacgg atgtttcgtt tcctccatcc atcccaccag ccgttccttc atggcaaatg
     1981 cctgttggag gtaccgcgca cgatcctggt tcgcgccgag gaggttgttg acttcttcag
     2041 ggtcacgagt aagatcgaac agcatgtcaa cctcgtcagc atggttactc cacacgtact
     2101 tccattcgca agtctgcgcc atgacgttcc agtgctcaaa ctgcgagaag actatcgggt
     2161 cgggttcagg agcgtcgccg gcgatccatg actgcagact ccgcccccga cggtcgcgcg
     2221 gtgtgacgcc cagatagtcc gatattgttg cgaagagatc ggtcaggcaa accggcgcat
     2281 gcacggtgct tcccacccga atgcgtccgg gcatgcgcat caaaagcggc acaccgactg
     2341 cttcgcggta gtgaacgaac ttcgtcatca gcccgtggtc tccaagcatc tcgccgtggt
     2401 ctgaggtgaa cacgacaagc gtgttgtcgg tgaggcccga ggtgtccaac gcatcgagaa
     2461 tgcggccaat gtgatgatcc acttcctgta ccgccgcata gtaccgcgcc atcaggagat
     2521 gcaggtgtct gcgttcgagg tcgtccatct gccatacgaa atccttgtac ggcgtgttca
     2581 tccgcgcgtc gtcaaacgtc tccggtgtcg ccatatcggc tgcccgaact gaatcgcaat
     2641 agggacgcgg aacgatgagc ggatcgtggg ggaaaaggaa agaacttgtg atcgagaaag
     2701 ggacgtcttt gagcctctcg atactcctca ttgtttcatc ggcaatcatg gcggaaattg
     2761 agtgctcagc acgcaccgaa tcccgtccga aagggagccc cggctccggg cgggtgtgac
     2821 cgcggcccag attgagagaa cggtacacgt ggtccatcgg gtcagggtcg tacggttgcc
     2881 cgctcagtgg cgagatgtac gcgtgggatg caccctggtg cggggaacca tacccttttt
     2941 cagccagata gccgcgatag aaggtatcga gggaaacaga tgtcgggagc cgcgagtaag
     3001 gtctgagcaa ggcttctgga gcatgccatt tgccgatgta ttctgccgta tagccgtgtg
     3061 cggtgagtgc gtcgtcgaat gtcgtcaaat cggaggcagg ggttctcaac ctgtcagggt
     3121 tggtaatgca tcccgtgtgg tggattggca aaccggtgag caccgatgct ctggctggaa
     3181 cgcagatcgg gctgggagtg atggcgttcg tgaagcgcac gccctccacg gcaagccgat
     3241 ccagggacgg tgtgtgaacg aaccggcttc ccatgcacga aagcgcgtcc ctgcgttgtt
     3301 ggtccgtcat gatgaagagc agattgggat ttctggcggt tctcacctca ggcttctcca
     3361 ttggcggtgg gcttttaagg tctccggtac catacctacg tccaatctgg cgcagggaca
     3421 atcccggacc gtcagcgaga gtccgcgaag ttccttcacg cgatttcctg cgtatcatag
     3481 tgtatccggg ccgtggttcg aactttgtct cgcgagggtg tcgatgggtg caggaagctt
     3541 cgtccggcga agggtgatct cgccgatcat ggacttgctg aagcagggag tgaccccgga
     3601 aaagatcgcg ctcagcatgg ccgtaggtgg cgcgctcggc gtgttccccg tgctgggcag
     3661 caccaccatt ttgtgcactc tcgcggcatt cctcttccgc ctcaacatgc cggcgattca
     3721 gatcgtcaac tacgtcatgt ttcccctcca gcttctgctc ctcatccctt tcatccgctt
     3781 cggggaatgg attttccacg cggcccccat cccattctcc gcctcggaga tggcggccat
     3841 gttccgttca gatttccagg gctccatggg cttgctgtgg gtcacagggt tacaggcaat
     3901 tgtcgcgtgg tgcgcattca tgccgccggc agccgtgctg gtctatctcg tgctcgcacc
     3961 gggcttgcga cgaattcagg ctcgctttcg gcggacggat ggtccttcct gatccagccc
     4021 ggttgcagga aa
//
LOCUS       DAOSOO010000258         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22419_length_4032_cov_3.766155, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000258 DAOSOO010000000
VERSION     DAOSOO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22419_length_4032_cov_3.766155"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="PLT86_13375"
     CDS             <1..2111
                     /locus_tag="PLT86_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT37647.1"
                     /translation="DVAVACLGGKSVVMYTNGEGGAIFHRQKKAPADTTFRFSGGFRP
                     RSVATADVNADGLPDLVVALAQDSVVSGLDRIVVLLNQRVSTGSSAYSVGADYVAGSG
                     VRDLFVGDLDPGDGGVEIVGASSVPGVAPVVLARAARVARAGEAIDSTINLLGFSPAD
                     GASNLGLRTPVSFWFDTEVKNMSALANLDSQWVQITGTIDNSGQTRRIPVSVAKQTRR
                     VNYASYTEYVLTPLTPYRPHETVTVSLTGRILSLQSNDRRTIALTNPRSWSFSAEGMR
                     IVSSIPADGAVHVPTTSGVSLRFNFWLDPASIDGALTILGASGTIPFAYTHAADTISS
                     VVTLTPQRAFEPYETVRVIGLPTLRDSTGRPTFAGDTVSFRATGPQVVATSPVNGATT
                     RLAGNTLTVSFNTRMSMDNPQAVLVVGEQSGAHPIASLSSPDGGYTAHVALAGNFIGG
                     EVVTATVTSAFVSAVSSFPLARPHVWRFSIQPSAPASLTKRDPVAGDLFAGGSVAAGR
                     FTAMDDGRGVLLSDPTGSLALFSGGSGTWTQAASLTGIPGRSCTRAGDLNGDGLLDIV
                     VAAADSDFVQVYFNASGAASTPAFEQPVRLPVGGQPVGVFIADLNGDGRPDIATANRA
                     TNDVSVLLNNGNKTFAEEVFVAAGNNPQAINGADLDGDGDIDLVVANATDNTITLIRN
                     LTGFREPPQTGAKRGNGGVR"
     gene            2108..2821
                     /gene="ispD"
                     /locus_tag="PLT86_13380"
     CDS             2108..2821
                     /gene="ispD"
                     /locus_tag="PLT86_13380"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT37648.1"
                     /translation="MIGEGLPVVAVVVAGGAGVRMGGGTPKQYRLLAGAPVLAWTLRA
                     FEKATDVDEVILVVPPSDLAFVGEAIVDAYRLTKIRRIVEGGATRQESTAAGVAAVEA
                     SDGVLLVHDGVRPFVSGEHIAAVVEAAWSTGAAILAVPVTDTVKRVDDRGCIEGTISR
                     ENLWAAQTPQGFRLSLFREAMARARREGFAATDEAMMVERMNHPVMIVPGDAENIKLT
                     VPEDFARAAQIAERRSRPC"
     gene            2815..3294
                     /gene="ispF"
                     /locus_tag="PLT86_13385"
     CDS             2815..3294
                     /gene="ispF"
                     /locus_tag="PLT86_13385"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT37649.1"
                     /translation="MLRIGQGYDIHPLAAGRRLVLGGVEIPSDVGLEGHSDADVLVHA
                     IMDALLGALALGDIGQHFPNTDERYRGVDSIRLLERVAELIRQHGARVVNVDSTVVAE
                     RPRVAPYVPAIRERISAALGIPASAISIKATTAEKLGALGRGEGISASAIALVGDGV"
     gene            complement(3306..>4032)
                     /locus_tag="PLT86_13390"
     CDS             complement(3306..>4032)
                     /locus_tag="PLT86_13390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT37650.1"
                     /translation="ELLLRFNPIARGAIKQARLVLAANTETRKDVERSGHVAVRLAWD
                     NALSPTYIPAARPQRARSEWLGLVWVGSLIPRKALRLALEAMAASSAPTRLTIVGDGP
                     LAQELPGWLKNLGIEDRVDWWGQVPWLEVKRAYMENDVLLFTSLRDTSGAQFLEAMAH
                     GMPVIALDHHGAHDWIPDNAGIKVPVTNPKETVAGLAAAIDRLWREPELREQMGNAAY
                     EYAKKQTWENRVREIAGLYEQFM"
BASE COUNT          702 a         1220 c         1334 g          776 t
ORIGIN      
        1 cggacgtcgc cgtcgcctgc ctcggcggca agtcggtggt catgtacacc aacggcgaag
       61 gcggggcgat tttccaccga cagaagaaag cacccgccga tacgactttc cgcttttccg
      121 gcgggtttcg gccgcgttcg gttgctactg cggacgtgaa tgccgacggc ctgcccgacc
      181 tcgtggtggc gctcgcgcag gattccgttg tgtcgggtct ggatcggatc gtggttttgc
      241 tcaaccagcg ggtatccacg ggtagttccg cctattcggt gggcgcggat tacgttgcgg
      301 ggagcggcgt gcgcgatctt ttcgtcggcg accttgaccc gggcgacgga ggggtggaga
      361 tcgtcggggc gtcgtccgtt ccgggtgttg cgccggtagt ccttgcgcgg gcggcaagag
      421 ttgcgcgcgc cggggaggcg attgacagca ccatcaacct gcttggtttt tctcccgcgg
      481 atggtgcctc caaccttggt ctccgcaccc ccgtgagttt ctggttcgac accgaagtga
      541 agaacatgag cgcgctggca aacctcgaca gccagtgggt gcagataaca ggcactatcg
      601 acaacagcgg gcagacgaga cgcattccgg taagcgttgc gaaacagacg cggagggtga
      661 attacgcgtc ctacacggag tatgtcctga cgccgctcac gccgtaccgc ccgcacgaaa
      721 cggtgacggt ttctctcacc ggccgcattc tgagtctcca gtcgaacgac cgccgcacga
      781 tagccctcac caatccccgt tcgtggtcgt tcagcgccga gggcatgcgc atcgtgagca
      841 gcatccccgc ggacggggcg gtccacgtgc ccacgacgtc cggcgtgtcg ttgcgcttca
      901 acttctggct ggatccggcg agcattgacg gtgcgttgac aattctcggt gcttcgggaa
      961 ccattccctt cgcctatacc catgcggcag acaccatttc cagcgtcgtg acgctcaccc
     1021 cgcaacgggc atttgaaccg tatgagacgg tccgggtgat cggactcccg accctgcgtg
     1081 attcgacggg gaggccaacg tttgcgggcg ataccgtctc tttccgggca accggtccgc
     1141 aggtggtggc aacgagcccg gtcaacggtg ccaccacacg gcttgccgga aacaccctca
     1201 ccgtttcgtt caacacgcgc atgtcgatgg ataatccgca ggcggttctg gttgtggggg
     1261 aacagagcgg cgcgcatccg attgcctccc tttcaagccc ggatggcggt tacacggcgc
     1321 atgtcgcgct ggccggcaat ttcatcgggg gcgaagtcgt cacggcgacg gtaaccagcg
     1381 cgtttgtgtc cgcggtgtcg tcgtttcctc tcgcgcgtcc gcacgtatgg cgattttcga
     1441 tccagccgtc ggcaccggcc agcctgacga aacgggatcc ggtggccggg gacttgttcg
     1501 ccggtggatc ggtggcagcg gggcgattta ccgcaatgga tgacgggagg ggtgtattgt
     1561 tgagcgaccc gacgggttcc ctcgcgttgt tcagcggggg atcggggacc tggacgcagg
     1621 cggcctctct caccggcatt cccggacgca gctgcacgcg ggcgggcgac ctgaacggcg
     1681 acggcctcct ggacatcgtt gtggcggctg cggattcgga cttcgtgcag gtgtatttca
     1741 acgcctccgg cgccgcatct acgcctgcat ttgagcagcc ggtgcgcctt cccgtcggcg
     1801 ggcaacccgt cggggtgttt atcgcggacc tgaatgggga cgggcggccg gacatcgcaa
     1861 cagcaaaccg cgccacgaac gatgtctccg ttctcctcaa caacggcaac aaaacgtttg
     1921 ccgaggaagt cttcgtcgcg gcgggcaaca acccgcaggc aatcaacggc gccgatctgg
     1981 acggcgatgg cgacattgac ctcgtggtgg cgaacgcgac cgataacacg atcaccttga
     2041 tccggaatct taccggtttc cgggaacctc ctcagaccgg ggcgaaacgt gggaacggag
     2101 gggtgcgatg atcggggaag gccttccagt tgttgcggtt gtggtggcgg gtggggctgg
     2161 ggtgcgcatg ggcggcggca caccgaaaca gtaccgtttg ctcgccggtg caccggttct
     2221 cgcatggacg ttgcgtgcct tcgagaaggc gacggacgtg gacgaggtca tactggtggt
     2281 gccccccagc gaccttgcat tcgtcggtga agcgattgtt gatgcgtacc ggttgaccaa
     2341 aatccggcgc atcgtggagg gaggagccac ccggcaggaa tccactgcgg cgggcgtcgc
     2401 ggcggtcgaa gcttccgatg gtgttctcct tgtccatgac ggtgttcggc cattcgtgtc
     2461 cggagaacac atcgcggcgg ttgtggaggc cgcgtggagc actggtgccg ccatcctcgc
     2521 cgtgccggta accgacaccg tgaagcgtgt tgacgacagg gggtgcatcg agggaaccat
     2581 ctcgcgcgag aacctgtggg cggcacagac gccgcaggga ttccgcctgt cgttgttcag
     2641 agaagcgatg gcccgcgccc gtcgggaagg gttcgcggcg acggacgaag cgatgatggt
     2701 ggaacgaatg aaccatccgg tgatgattgt ccccggcgac gcggagaaca tcaaattgac
     2761 ggtgccggag gatttcgcgc gtgctgctca gattgcggag aggaggagca ggccgtgctg
     2821 agaatcggac agggatacga catccacccg ctggctgcgg gcaggaggct cgttctcggc
     2881 ggggtggaaa tcccttcgga cgtgggattg gaaggccatt cggacgcgga cgtgctggtg
     2941 cacgcgatca tggatgccct gctgggtgcg ctggcgctgg gggatatcgg ccagcatttc
     3001 ccgaacacgg acgagcggta ccggggcgtc gacagcatcc ggttgctgga aagggtggct
     3061 gagctgatcc ggcagcacgg ggcaagggtg gtcaacgtgg attcaacggt ggttgccgaa
     3121 cgcccgcgtg tcgctcccta tgttcccgct atacgggaac ggatcagcgc ggctcttggc
     3181 attcccgcga gcgcaatctc gatcaaagcc accactgcgg aaaagctggg cgcactcgga
     3241 cggggggagg ggatttcagc gagcgccatt gcactcgttg gggacggtgt gtagccgaca
     3301 acgcgtcaca taaactgctc gtaaagaccc gcaatctccc gcactctgtt ttcccacgtc
     3361 tgtttcttcg cgtactcgta cgcggcgttg cccatctgct ccctcaactc cggttcccgc
     3421 cagagcctgt caattgccgc ggccagcccg gcaaccgttt ccttcgggtt ggtaaccgga
     3481 accttgattc cggcattgtc cggaatccag tcgtgtgccc cgtggtggtc gagcgcaatg
     3541 acaggcatac catgggccat ggcttccagg aactgggcgc cggacgtgtc acgaaggctc
     3601 gtgaacaaca gcacgtcgtt ttccatgtac gcgcgcttca cctcaagcca ggggacctgc
     3661 ccccaccaat cgacgcggtc ctcaatcccc agattcttca accaccccgg gagttcctgc
     3721 gcaaggggac catcgcccac gatggtgagt ctggtcgggg cgctcgacgc cgccatcgct
     3781 tcaagggcca accgcaaggc ttttctcggg atcaagctgc cgacccaaac cagccccaac
     3841 cattctgacc gcgctctctg aggtctggcc gctggtatgt acgttgggga aagcgcgttg
     3901 tcccatgcca gacgcacagc tacatgcccc gatctttcaa cgtccttccg agtttctgta
     3961 ttcgccgcca gaacaagccg tgcctgcttg atcgccccgc gtgcgattgg attgaaccgg
     4021 aggaggagtt cg
//
LOCUS       DAOSOO010000259         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22431_length_4030_cov_5.097107, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000259 DAOSOO010000000
VERSION     DAOSOO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22431_length_4030_cov_5.097107"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..77)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(212..1657)
                     /locus_tag="PLT86_13395"
     CDS             complement(212..1657)
                     /locus_tag="PLT86_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37651.1"
                     /translation="MTLRGHEERPNVVVILSDDQGPWAMRCTGNSELETPNLDRLAAT
                     GMRFDRFFCTSPVCSPARASLLTGRIPSCHGVHDWLAAGNTIAKYEPARKGEFIDFLV
                     GQPAYTDELAKAGYACGLSGKWHLGDCHRPRDGFVFWDAHAKGGGPYYRAPMVRHGEV
                     YEEPNYVTDAITTNALRFLEQRKSHSQPFYLSVHYTSPHSPWDRENHPKELFDQYHDG
                     CPFTSTPDLMPAPDWVKHRSIPVDDAGSRRQKLSGYYAAITAMDTNIGKIIDWLEENG
                     KRENTLVVFTSDNGMCMGHHGLWGKGNASFPLNMFEESVRVPFLASHPGVIEGGAVNS
                     DLLSQYDFSSTLLEYLGISTPSDPISPGRSFCELLKGRRRDPHEHLVVFDEYGPVRMV
                     RTREWKYVHRYPYGPNELYHLINDPAETENRISDPAARPVLREMRERLHEWFATYSDP
                     DRNGATEPVTGRGQTGLCGSRARGEDVFIPF"
     gene            complement(1722..2798)
                     /locus_tag="PLT86_13400"
     CDS             complement(1722..2798)
                     /locus_tag="PLT86_13400"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOT37652.1"
                     /translation="MANLWGKHWTKRELLERVGDISQIADVRKSVLADGNNTGVEVFD
                     FATGSGFAFTVAAGRAMDVVAASYQGKALAWRSSVGDPHGSFYDPEGLEWLRQFFGGL
                     VATCGLSYAGAACTDEGKKLGIHGRIGNVPAKRANFGGYWEGDEYILWASGVMRESGL
                     FQDELELQRVISTKLGANSFTIRDKVVNIGWHTTPLMILYHCNYGFPVVANGSEVLTP
                     AVKVTPRDADAREGAETYFRLHEPVKDYREKCYYHDVNAGPDGRTAIGIVNREMGIGG
                     YIRYRPEQLPKLIQWKQMGQGWYTCGLEPANCLVEGRDKDRATGRLQFIEPQEERIFE
                     LELGVLTNQEEIAQFEAEVNRIRG"
     gene            complement(2912..>4030)
                     /locus_tag="PLT86_13405"
     CDS             complement(2912..>4030)
                     /locus_tag="PLT86_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOT37653.1"
                     /translation="DQHLTVKGTMNIRSVSSYVVHGKCFVVVQTEEGVEGVGECSPMH
                     SETTARFVAEVLGPRIIGKNALETDKLWHVMMLDTYKLGVQGIQPEAVAGVDIALWDI
                     RGKATGQPLCMMLGGCYREHIPMYASIGGGATMTPREMATKVEKAVHLGCKGVKIRMD
                     WGVARQDRGPEKDLRMVEECKQVCGTTVPLSFDANNGYSVPTAIRQGRKFEALGLRHF
                     EEPVAQFDYEGLGKVADALDLPVSAGEHEYTRWQFRDLILNARVDIVQPDVVKCAGIT
                     ETMKIATLAEVFNRLFLPHQTQPSIGTAVNVHICAALHWCDLPQEYTGQSPELDELFE
                     EPLVFYDGAITVPLRPGHGLVLKKSALDGATRFSTSAN"
BASE COUNT          800 a         1221 c         1068 g          941 t
ORIGIN      
        1 cgtaccggat tttcaccggt ttccctattc tccgaggcgt gttcccacgc gccggcacct
       61 gagctgaggt gaaatgacca aatggaagat gagttggatc ctgtgttgtg tcaatagcct
      121 cggctacgaa aaatctgtca ccccacggag tgctgataag aatgcttatg taatgtcaac
      181 ttttcgcgcc ggggcgtcta gacctgaacg actagaaagg tatgaacacg tcctctcccc
      241 ttgcacggct gccgcacaac cctgtctggc cccttccggt aaccggttcg gtcgctccat
      301 tgcggtccgg gtctgagtag gtcgcgaacc attcatgcaa tcgttcccgc atctctctca
      361 acacaggacg cgccgccggg tcactgatcc gattctcggt ttcggcgggg tcattgatga
      421 ggtgatataa ctcgttcggg ccatacgggt aacggtggac gtatttccac tcacgcgttc
      481 tgaccattcg tacagggccg tactcatcga aaacaacaag gtgttcatgt gggtccctgc
      541 gtcggccttt gagcagttca cagaaactcc tgccgggaga tatggggtca gacggggtgg
      601 agattcccag gtattcgagg agagtggacg aaaagtcgta ctggctgagc aaatcgctgt
      661 ttacagcgcc gccctcaatc actccggggt gcgatgccaa gaacggtact ctgaccgact
      721 cctcgaacat gttaagtgga aaacttgcgt ttccctttcc ccataggccg tgatggccca
      781 tgcacattcc attatcggaa gtaaacacga cgagagtgtt ttctcgcttg ccattctctt
      841 ccagccaatc gatgatcttg ccgatgttgg tgtccatcgc agttattgcg gcgtagtagc
      901 cggaaagctt ctggcgcctc gagccggcgt cgtccacagg gattgaacgg tgtttcaccc
      961 agtccggagc gggcatcaaa tccggcgtcg acgtgaaggg acagccgtca tggtattgat
     1021 cgaaaagctc tttcggatga ttctcgcggt cccaagggct gtgtggtgag gtgtaatgca
     1081 cactgaggta aaacggttgg ctgtgcgatt ttcgctgctc gagaaaccgc aaagcgttcg
     1141 ttgttatcgc gtcggtaacg tagttcggtt cttcgtagac ctccccgtgt cgcaccatgg
     1201 gggcgcgata gtacgggcct ccacctttgg cgtgggcatc ccagaacacg aacccgtccc
     1261 ttgggcggtg gcagtcgccc agatgccatt tcccgcttaa cccgcacgcg taccctgctt
     1321 ttgccagttc gtccgtgtac gctggttgtc cgacaaggaa gtcgatgaac tccccctttc
     1381 tcgcgggttc atacttcgcg atggtgttgc cggcggcaag ccagtcgtga acaccgtggc
     1441 aggacggaat ccgaccggta agcagagagg cacgcgccgg agagcacacg ggcgaggtgc
     1501 agaagaaacg gtcgaaccgc atcccggtgg ccgcgagcct gtcaaggttg ggggtttcca
     1561 gctcgctgtt tcccgtacac cgcatcgccc acgggccctg gtcgtccgag agtatcacga
     1621 cgacattcgg acgctcttca tgcccgcgca acgtcatcct tcccgccttt cattccggtc
     1681 gggacacctg ccagtttgtt ctaagagcga aagtcgcgac atcacccgcg gattcgattg
     1741 acctccgcct cgaattgtgc gatttcctcc tggttcgtca gtacgccgag ttcgagctcg
     1801 aaaatccgtt cttcctgcgg ctcgatgaac tggagtcttc ccgttgcccg gtccttatcc
     1861 cgtccctcaa caagacagtt ggcgggctca agtccgcacg tgtaccaacc ctggcccatc
     1921 tgtttccatt gaatcagttt gggaagttgt tccggcctgt agcgtatgta cccgccgatc
     1981 cccatttccc ggttcacgat tccgatagcg gtacgtccgt ccggtccggc gttcacgtca
     2041 tggtagtagc atttctcgcg gtaatccttc accggttcat gcaaacggaa atacgtttcg
     2101 gcgccctccc gcgcgtcagc atcccgcgga gttactttga ccgcaggggt cagtacttcc
     2161 gagccattcg caaccaccgg gaacccgtag ttgcaatggt agagaatcat cagaggtgtg
     2221 gtatgccacc cgatattcac gactttatcg cgtatggtga acgaattcgc ccccagtttc
     2281 gtcgatatga cccgttgcaa ctcaagctca tcctggaaaa ggccgctttc gcgcatcacg
     2341 cctgaagccc agagtatgta ctcatcgcct tcccagtacc cgccgaaatt cgctcgtttg
     2401 gccggaacgt tgccaatacg tccatgtatc ccgagttttt ttccttcatc ggtgcatgcc
     2461 gcccctgcat agctcagacc gcacgtcgcg acgagaccac cgaaaaactg gcgcaaccat
     2521 tccaggcctt ccgggtcgta gaaggatccg tgggggtctc cgacggagga gcgccatgcc
     2581 agagcctttc cctggtaact tgccgcgacc acatccatcg cgcgtcccgc tgcaaccgtg
     2641 aacgcgaagc ccgaacctgt cgcgaaatcg aaaacctcca cgccggtgtt gtttccatct
     2701 gccagcacgc tcttgcgcac atctgctatc tgggatatat caccgacacg ttccaaaagc
     2761 tccctcttcg tccagtgttt tccccaaaga ttcgccattt ctctgctgtc ctccaaaatg
     2821 gtagtggtgt tttcagttgc tgtccatgta ctcgaattct acccgatgat ctgggaaagg
     2881 gcacacaact gtctgcctct ggttgcgatc ctcaattggc tgaggtggaa aagcgcgttg
     2941 ccccgtcgag cgctgacttc ttcagaacaa gcccgtgtcc agggcggaga gggacagtaa
     3001 tagccccgtc gtaaaaaacg agcggttcct cgaaaagttc gtccaattcg ggtgattgac
     3061 cggtgtactc ctgtggcaaa tcgcaccagt ggagcgcggc acatatgtgc acgttgacgg
     3121 cggtcccaat cgacggttga gtctggtgcg ggaggaatag cctgttgaag acctccgcca
     3181 aggtcgcaat cttcatcgtc tccgtgatgc cggcacactt caccacgtcg ggttgaacga
     3241 tgtccacccg cgcgttcaaa atcaaatcac gaaactgcca ccgggtgtac tcgtgctcgc
     3301 ccgcggaaac aggcaagtcg agtgcgtcgg ctaccttccc gagcccttca tagtcaaact
     3361 gagcgaccgg ctcttcgaaa tgccgcaacc ccaacgcttc aaacttgcgc ccctgtcgga
     3421 tggcagtcgg caccgagtac ccgttgttag catcgaagct cagaggaacc gtggtcccac
     3481 atacctgctt gcattcctcc accatgcgaa ggtctttttc gggccctctg tcctgtcggg
     3541 caacgcccca atccatgcgg atttttacac ctttacatcc aagatgtacc gccttttcaa
     3601 ctttcgttgc catttcgcgc ggcgtcattg ttgctccgcc cccgattgac gcgtacatgg
     3661 ggatgtgctc tcgatagcac ccaccgagca tcatgcacaa gggctgcccg gtagccttgc
     3721 cgcgtatgtc ccacagggcg atgtccacac ccgcaaccgc ttcgggttgg attccctgaa
     3781 cgccgagttt gtacgtgtcc agcatcatca catgccagag cttgtccgtt tccagcgcgt
     3841 ttttcccgat tattcgggga ccgagaactt ccgccacgaa gcgcgccgtt gtttcgctgt
     3901 gcatggggct gcactcgccg acaccttcta ctccctcttc ggtctgcacg acgacgaaac
     3961 acttaccgtg gacgacgtat gaagaaacac ttctgatgtt catggttccc ttcacggtca
     4021 ggtgctgatc
//
LOCUS       DAOSOO010000260         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22496_length_4022_cov_6.607008, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000260 DAOSOO010000000
VERSION     DAOSOO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22496_length_4022_cov_6.607008"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..963
                     /locus_tag="PLT86_13410"
     CDS             397..963
                     /locus_tag="PLT86_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37654.1"
                     /translation="MIGPFVVRFLAIMGIFIPGVNQQVSLLDPFVELRGDRIAVSTAL
                     QKPLSTDLLKIIDSGTPVTLTYVCRLRTSTDNPAAAPDTTVSHRVVKDLASGTYEIDT
                     GTRKILTSRITEYSLLFRLETVPLWNINVLSNSGFYYVEVSARFEPIVIHATGERYDL
                     MALWSYRPPTARSKLFNRFIVERRRVEP"
     gene            960..2165
                     /locus_tag="PLT86_13415"
     CDS             960..2165
                     /locus_tag="PLT86_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT37655.1"
                     /translation="MSLRARFILSAMILLLAGMLPMGWFAMRSIDKTIRVWHDAQVGA
                     ALQQSLTHVENEAVRRQVNEALIRYNQLGALQRPMQWQLVIVGLTLAFAIVVLASLAA
                     WFLAVQLTRPLNSVAKAAEDIARGDLSHTVPPARIPEIAALVDTFNAMVVSLRQSRAA
                     LARAERRAAWQDIARAIAHEIKNPLTPMRLTTQHLRERFQENRPRFEETFIRSTDMIL
                     TEIDRLERLANAFSTFAKMPAPVMEPLDLRVLIGQTTDLFPAERDGGRLIVHLPETPV
                     TVSGDLEQLKQALLNLVKNAFEAPGEGERRVELSLSSTATDAIVEVKDDGTGIPPDML
                     ERLFQPYITTKSGGSGIGLAIVDRVITDHQGRVTAQNMEQGGALFRVWLPLQNPGFIR
                     EDTYAHPGY"
     gene            2146..3537
                     /locus_tag="PLT86_13420"
     CDS             2146..3537
                     /locus_tag="PLT86_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT37656.1"
                     /translation="MRILVIDDEPNIRRSLRDILEDEGYEVDEAGDGETGLRLLERQP
                     ADIVLLDVKLPKADGVTILGDVKARLPHTEVIMISGHSDIETAVDALRKGAYDFLEKP
                     LSLPKVRLAVAHAAEKISLRKRAREQAAARFQPLIGTSAAMSAVRAMISRVAPTTSSV
                     LIRGESGTGKELAAQQIHVQSNVSDGPFVQTNCAAIPHDLIESELFGHEKGAFTGAAA
                     RRLGKFEQANNGTLFLDEIGDMDIAVQAKVLRALQNGEFQRVGGTETLFAKVRVVAAT
                     NKNLEKEVEEGRFREDLFFRLNVVPIFMPPLREHPEDIPELARHFLARFCMENGREQI
                     RITDEALERLKTFSFRGNVRELKNLVERLAIFSTGNEITLATVLAQAHPVLTGQNTHF
                     LRSRPLAEAKNELEKTYIETQLNLYGWDIVRTAEALDILPNNLHRKITQLGIQRPHRK
                     HGTKDEEEAGDSE"
BASE COUNT          829 a         1254 c         1189 g          750 t
ORIGIN      
        1 catgaaaccg aatgggaggg ccgcggaaat gacggaaatg aagtagggag cggagcctac
       61 gtcatccgac tcgcttcacc tgcgggggtc gtgacgacaa aactcaccct gttgcggtga
      121 cctgttttgg agtacggcgg cccaccagtg gtggggcttt cccgccgtag gcgggacggc
      181 attcttgcct gccctgcggc atagccgaca cctgctcact cccgcatccg aaccggccgt
      241 atgctttatc agaacactcc gtccgccgcc gccgcaatgc cctccccgaa tatgaatccc
      301 cctccgcgag tagtacatta ttgacaacct cttaggaaag cgtgaaccat tgcgggcgtg
      361 tattgacgcg cgtaccggct atcccgcggg gaaagcgtga tcggaccttt tgtcgttcgg
      421 tttctggcga tcatggggat attcatcccc ggtgtgaacc agcaggtctc cctcctcgat
      481 ccgttcgtcg aacttcgcgg cgatcgcatc gccgtgtcta ccgcccttca gaaaccactt
      541 tcgaccgatc tgctgaaaat cattgacagc ggcacgcccg tcaccctgac ctatgtctgc
      601 cgcctgcgta cgtcaaccga caaccctgcc gctgcgccgg atacgacggt ttcccatcgc
      661 gtggtaaagg acctcgcgtc cggaacgtat gaaatagaca ccggcacgcg caaaatcctc
      721 acctcgcgca tcacggagta ttcgctcctg tttcgtctcg aaaccgtgcc gctctggaac
      781 atcaacgtgc tttccaattc cggcttttac tatgtggagg tttcagcgag gttcgaaccg
      841 attgtcattc atgccacggg ggagcggtac gacctcatgg cgctctggag ctatcgtccc
      901 ccgacagcgc gcagcaaact gttcaatcgc ttcatcgtgg aacggcggcg ggtagagcca
      961 tgagtctccg cgcgcgattc attctctcgg cgatgatcct cctgctcgcc ggcatgttgc
     1021 cgatgggctg gttcgccatg cggtccatcg acaaaaccat ccgcgtctgg cacgatgcgc
     1081 aggtcggagc ggcgttgcag caaagcctca cacacgtgga gaatgaagcg gtcaggcgcc
     1141 aggtgaacga ggcgctcatc cgctacaacc agctcggggc gttgcagcgg cccatgcagt
     1201 ggcaactcgt cattgtcggg ttgacgctgg cattcgccat tgtcgttctg gcatctctgg
     1261 ccgcatggtt tctcgccgtt cagcttaccc ggccgctcaa ctcggttgcg aaggccgccg
     1321 aggatattgc ccgcggcgat ctttcgcaca cggttccccc ggcgcgaatt cccgaaatcg
     1381 ccgcgctggt ggatacgttc aacgcgatgg tggtcagtct gcgacaatcg cgcgccgcgc
     1441 tggcacgggc ggaacggcga gccgcgtggc aggatatcgc ccgcgccatc gcccacgaga
     1501 tcaagaaccc ccttaccccc atgcgtctga ccacccagca tctgcgcgaa cggttccagg
     1561 aaaatcgccc gcgtttcgag gagacgttca ttcgctccac ggacatgatc ctgacggaaa
     1621 ttgaccggct cgagcggttg gccaacgcat tttcaacctt cgcgaagatg ccggctcccg
     1681 tcatggagcc gctggacctg agagttctga tcggtcagac aacggacttg ttccccgcgg
     1741 agcgcgacgg tggtcggctc atcgtccacc ttccggagac cccggtcact gtatcaggcg
     1801 atctggagca actgaagcag gcgttgctga atcttgtgaa aaatgccttc gaggccccgg
     1861 gcgaggggga gcgccgggtg gagctcagcc tctcctctac cgcgacggat gcgattgtgg
     1921 aagtgaagga cgacggaacg ggcattccgc cggacatgct ggaacggttg ttccagcctt
     1981 acatcacgac aaaatcggga gggtcaggga tcgggctcgc cattgtcgat cgcgtaatca
     2041 ctgaccacca ggggcgtgtc accgcgcaga acatggaaca gggtggcgcg ttgttccgcg
     2101 tatggctgcc gctccagaac ccggggttta ttcgagagga tacgtatgcg catcctggtt
     2161 attgacgacg aaccgaacat ccgacgatcg ctccgcgata tcctggagga cgaagggtac
     2221 gaggtggacg aagccgggga cggcgaaacc ggactgcgtc ttctggaacg ccagccggcc
     2281 gatatcgtgc tccttgacgt gaagcttccg aaggctgacg gcgtgacgat tcttggcgat
     2341 gtcaaagcac gcctgccgca cacggaagtc atcatgatat ccgggcactc ggatatcgaa
     2401 acggcagttg atgcactgcg caaaggcgcc tacgatttcc tggaaaaacc gctcagcctg
     2461 ccgaaggtgc gccttgctgt cgcccacgcc gcggaaaaga tctcactccg aaaacgtgcc
     2521 cgcgagcagg cggcagcccg attccagccg ctcatcggta ccagtgctgc aatgagcgcg
     2581 gtgcgcgcga tgatcagccg cgttgcgccg acaacttcca gcgtactcat ccggggagaa
     2641 agcggcaccg gcaaagaact tgccgcccag cagattcacg tccagagcaa tgtttcggat
     2701 gggccgttcg tacaaaccaa ctgcgccgcc attccccacg atctcatcga aagtgagctg
     2761 ttcgggcatg agaaaggggc atttacgggt gccgcggcgc gccggcttgg caaattcgag
     2821 caggcgaaca acgggacgct gttccttgac gaaatcggcg acatggatat cgccgtgcag
     2881 gcaaaggtgt tgcgcgcact gcagaacggc gaatttcaaa gggtcggagg cacggaaaca
     2941 ttgttcgcca aggtccgcgt cgtcgctgcg accaacaaaa acctggagaa agaagtcgaa
     3001 gaaggtcgct tccgcgagga cctgtttttc cgcctcaacg tggtgccgat cttcatgcct
     3061 cccctgcggg agcatccgga agacatcccg gagctggcgc ggcacttcct cgcccgcttc
     3121 tgcatggaaa acgggcgtga gcagatccgt atcactgacg aggctctcga gcgtctgaaa
     3181 actttctcct tccgggggaa tgtgcgcgag ctcaagaacc ttgtggaacg actggcgatc
     3241 ttctccaccg gcaacgaaat cacccttgct accgttctcg ctcaggcaca cccggtgctg
     3301 accggccaga acacgcactt tctccgctca cgacctctcg ccgaggccaa aaacgagctg
     3361 gagaagacgt acatcgaaac gcagttgaac ctctacgggt gggacatcgt ccgcacggcc
     3421 gaagcactcg acatcctgcc gaacaacctc caccggaaga tcacgcagct cggtatccag
     3481 cgcccgcacc gcaaacacgg cacgaaggac gaggaagagg caggcgactc agaatgacgg
     3541 cacgcgggcg ggactccagg cttcccgtag cacgggcatc ctgcccgtgc cagattcccg
     3601 cgggcggtca tgatggctgc cgcacggttt ctgcggcctg ctcatgcgca agtccgccga
     3661 agcaatctgc acgcgtcatt cccgcgcagg cgggaatcca gaaaccccgc aaaaacacac
     3721 tcccgcgtgc cggttccttc ttgcgaagcg ttgtcgggta ctgcgccgat gaagaaccga
     3781 tggcgtcaac gtgaattacc gccttcgcgg gaatgacggg acgcctggtg tgcgggtacg
     3841 gggcaccgtg cgccgcacgc acgccaggct gacggcagcc acaccccgct gcgccaaaag
     3901 gagagagtgc gcggcccttc ccgaggcgga aacggctcag attggctcgg gtaccttatt
     3961 ccgaacttga ccgtcattct gagcgaagcg aagaatctgt tcctgaacta gttcgggaaa
     4021 ta
//
LOCUS       DAOSOO010000261         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_22704_length_3994_cov_5.364306, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000261 DAOSOO010000000
VERSION     DAOSOO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22704_length_3994_cov_5.364306"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLT86_13425"
     CDS             <1..630
                     /locus_tag="PLT86_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37657.1"
                     /translation="VIFASDHGDMLGDHSLLVKGAYFFDPNIRVPLIMRWPAGFAGGV
                     RVSSLVQIQDLAATVLAAAGLDPDDVRAKAPEAENLLPLATGEKQRVRDWAACIYRNT
                     GIKEGGVYFDPEIHATMFMDERHKLGVYHDVSEAPGTATEGVLYDMQADPNELHNLWA
                     DPAYRSVRLGLTERLLDWQVAQELRYGRRGSDLQPNASQRIIQDPTRDK"
     gene            complement(873..1166)
                     /locus_tag="PLT86_13430"
     CDS             complement(873..1166)
                     /locus_tag="PLT86_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37658.1"
                     /translation="MSGQREGFLHGGGLGAKGKLHKVGRFVPKPPRRVEDNAPYRTAT
                     TVDAMDRVDAASRSPSGTTYARRTECCIEEPGAAPRPALRAGLCYFAPLALAG"
     gene            1237..2334
                     /locus_tag="PLT86_13435"
     CDS             1237..2334
                     /locus_tag="PLT86_13435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT37659.1"
                     /translation="MNTPLAFSFHLPTRVVFRRGAVAMVPALDEVKGKRCMLLTLSSH
                     APEEAITELGAAASHLTLIRGVEENPRHSYVCALAEQAVAEGIETVVAIGGGSAMDAA
                     KAVAWFAANPGWRIGEERQPAIPAMKLVAVPTTSGTGSEVSPFSVLTDDETHQKRFLK
                     HPALMPAVALCDPLLTVTMPVQVTADTGIDALSHAVEAYLSVLCQGFMEGIALESCRL
                     VAENLPVVLENDPGDMTARENLMLAALDGGIVLANCGTVFVHALGYCLTAKFGYTHGK
                     ANAILLAGFVEHLAERGSPRAQAILDIFGGSLRAFIERCGVPQKLPPGSVTSDEFAAW
                     VSTGYNAYGRVNGVIALDRSDVENVLNRVIA"
     gene            2386..3087
                     /locus_tag="PLT86_13440"
     CDS             2386..3087
                     /locus_tag="PLT86_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOT37660.1"
                     /translation="MKLYGAGSLDDIKKCTELGVVGILTNPQGFEQYFKGEMTLDEIT
                     QAIVNATDLPVFIQIHGQTTDALVEKARKLNAIAPKQVGFKLISDEKGFQAIRRLQKE
                     GINCIATALFSVSQAAIAACVGAFGICPFVSRARDFGIDASELLLSIREGYDRLATAP
                     EIVVVSLKSVADVDLALASGADAVAMRYPLIQQMMQHPLSTKAELLFAKNWANVKGED
                     IGYLRHALTAEGISE"
     gene            3088..>3994
                     /locus_tag="PLT86_13445"
     CDS             3088..>3994
                     /locus_tag="PLT86_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT37661.1"
                     /translation="MAPIFGFGSATLDFRINTADFGDNYRDKLLARETKVFGGGSAAN
                     CLVQVARLSRQAVGSSLRPAWLGKLGRDWIGDGIVSGLEEEGVDCSAALRDATVCSPF
                     NVAVYAEWRRVGGYLLPNALGTLTRDEVRQLASVPEPGDWCIAEIGEIPLETTREFCQ
                     TAHTRGIHLLIDVDLDPQRQCVGDPSLISAILRCADVLAPNRAALRSLYGEIRAPGLV
                     DALAAEYGVSIVITSGADGAYYRAPGQPTRHVPAIPVDVVDTVGAGDAFHGGLLYALS
                     RGETLADAVLLGTRCGAAACLKFGAR"
BASE COUNT          712 a         1374 c         1232 g          676 t
ORIGIN      
        1 gtgatcttcg cgtccgatca cggcgacatg ctgggcgacc acagcctgct ggtgaagggg
       61 gcctatttct tcgatccgaa catccgcgtg ccgcttatca tgcgctggcc agcgggcttc
      121 gccggcggag tgcgcgtctc ttcgctcgtc cagattcagg acctcgccgc caccgtattg
      181 gcagccgccg ggctcgaccc ggacgacgtc cgcgccaaag cgccggaagc cgagaacctc
      241 cttccgctgg caaccggcga aaagcagcgg gtgcgcgact gggccgcctg catctaccgg
      301 aacaccggca tcaaagaggg cggcgtctac ttcgatccgg aaatccacgc gaccatgttc
      361 atggacgagc gccacaaact gggggtatac cacgatgtga gcgaggcccc cggcaccgcg
      421 acggaaggcg tcctgtatga catgcaggcc gacccgaacg aactccacaa cctctgggca
      481 gacccggcat accgctcggt caggctcgga ctgaccgagc ggctgctcga ctggcaggtg
      541 gcgcaggagc tgcgctacgg ccgccgcggg tctgacctcc agccgaacgc atcccagcgg
      601 atcattcagg acccgacacg ggacaaatag cccgagtcag gcagcccttt ccaccctgtc
      661 caccgcgtcc accgcacgcc cggttcgcag ggccgggcgc ctacaatccg tcattcccac
      721 gcgctcaacc gagcgacggg gaagcgggaa tccagcgggc caacacgcac ggtcacgaca
      781 aagcgtgacc ctccagccgc tgctcgtgcc tgccctggac gttgccccgc gctgaaatgc
      841 cccggccctt caggccttac tcggagcatt catcacccgg caagcgccaa cggcgcgaaa
      901 tagcaaagcc cggctcgcag ggccgggcgc ggcgccgctc ccggctcctc gatacaacac
      961 tccgtccgcc gcgcgtacgt cgtgccactc ggagaccggg acgccgcgtc cacgcggtcc
     1021 attgcgtcca ccgttgtggc tgtccggtag ggcgcgttgt cctcaacgcg acgcggcggc
     1081 ttgggaacaa accgccctac cttgtgcaat ttccccttgg ctcccagacc tcccccgtgg
     1141 aggaatccct ccctttgtcc actcatcgcg tcggtggtac gttgggacac aagaccctga
     1201 gcacccagac acgcatcact tgaaggctga taccccgtga acacacctct ggcgttttct
     1261 ttccatcttc ctactcgcgt ggtgttccgc cgcggcgccg tcgcaatggt gcctgcgctg
     1321 gatgaggtca aaggcaagcg gtgcatgctg cttaccctca gctcccacgc tcctgaggag
     1381 gccatcaccg aactgggcgc cgccgcttcc caccttaccc tcattcgtgg cgtggaggag
     1441 aacccccggc actcgtacgt gtgtgccctc gccgaacagg cggttgccga ggggatcgag
     1501 acggtcgtcg ccatcggcgg tggcagcgcc atggatgccg ccaaagccgt cgcctggttc
     1561 gcggcgaacc cgggctggag gatcggcgaa gagcgccaac ctgctatccc tgccatgaag
     1621 ctggttgccg tgccgacaac ctccggcacg gggagcgagg tgtccccgtt cagcgtcctc
     1681 accgacgacg agacgcacca gaagcgcttc ctcaagcatc cggccctcat gccggcagtc
     1741 gccctgtgcg atccgctcct cactgtcacc atgccggtgc aggtcaccgc tgacaccggc
     1801 atagacgccc tctctcatgc ggttgaggcg tatctgtccg tgctctgcca gggcttcatg
     1861 gaggggatcg cgctggaatc ttgccgcctc gtcgccgaaa acctccccgt ggtgctggag
     1921 aacgaccccg gcgacatgac ggcccgtgag aacctcatgc tggccgctct cgacggcggc
     1981 atcgtgctgg caaactgcgg cacggtgttt gttcacgcgc tggggtactg cctcaccgcg
     2041 aaattcggct acacgcacgg caaggcgaac gcaatcctgc tggccgggtt cgtggaacat
     2101 ctggcggagc gcggttcgcc ccgcgcacag gcaattctcg acattttcgg cggatctctc
     2161 agagccttca tagaacgctg cggcgttccg caaaagctgc cacccggcag cgtaacgagt
     2221 gacgagttcg cggcgtgggt cagcaccggt tacaacgcat acggccgggt gaacggcgtc
     2281 attgcgcttg accgcagcga cgtggagaac gtcctcaatc gtgtgatcgc ataggaaccg
     2341 gccaaccctg tgcgcggcgg gttccagggt gaaggaggca caacgatgaa gctgtacggc
     2401 gcggggtcac tggacgacat caagaagtgc actgaactgg gtgttgtcgg catcctcacg
     2461 aatccgcagg ggttcgagca gtatttcaag ggcgagatga cccttgacga aatcactcag
     2521 gccattgtca acgccaccga tctgccggtg ttcatccaga ttcacggcca gacaaccgac
     2581 gcacttgtgg agaaggctcg taaactgaac gccatcgcgc cgaaacaggt cgggttcaag
     2641 ctgatctccg acgaaaaggg attccaggcc attcgccgcc tgcagaaaga gggaatcaac
     2701 tgcatcgcca cggcgctgtt ttccgtatcg caggccgcca tcgccgcgtg cgtcggagca
     2761 ttcggcatct gccccttcgt ttcccgcgca cgggatttcg gcatcgacgc ctcggagttg
     2821 ttgctctcca tccgcgaggg gtatgatcgc ctcgccacgg cgccggaaat cgtcgtcgtc
     2881 agcctgaaaa gcgtcgccga tgtggacctc gccctcgcat cgggcgcgga tgcggtcgcc
     2941 atgcgctacc cgctcatcca gcagatgatg caacacccgc tcagcacgaa ggcggaactc
     3001 ctcttcgcga aaaactgggc gaacgtgaaa ggcgaagaca tcggatatct gcgccacgcg
     3061 ctcaccgcag aaggcatatc agagtaaatg gcccccatct tcggcttcgg cagcgcgacg
     3121 ctcgacttcc gcatcaacac cgcggacttc ggcgacaact accgcgacaa gctcctcgcc
     3181 cgcgaaacga aggtgtttgg cggcggctcg gccgcaaact gcctcgtaca ggttgcccgg
     3241 ctctcccggc aggctgtcgg ctcgtcgctg cggcccgcct ggcttggcaa gctcgggcgc
     3301 gattggatcg gcgatggcat cgtgtcgggc ctcgaggagg agggcgttga ctgctccgcc
     3361 gcgctgcgag acgccaccgt ctgctccccc ttcaacgtgg cggtgtacgc agaatggcgg
     3421 cgcgtcgggg gctacctgct gccgaacgcg ctgggcacgc tcacccgcga tgaggtgagg
     3481 cagctcgcct ccgtccctga accgggcgac tggtgcatcg ctgaaatcgg cgagatacca
     3541 ctggaaacaa cccgcgagtt ttgccaaacc gcgcacacgc gaggcattca tctgctcatc
     3601 gacgtggacc tcgacccgca gcgccagtgc gtcggcgacc cctcgctcat cagcgccatt
     3661 ttgcgttgcg cggacgtgct ggcacccaac cgggccgcgc tccgttcgct gtatggcgag
     3721 atccgcgctc ccgggctcgt ggacgcgctg gcagccgaat acggcgtttc gatcgtcatc
     3781 acatcgggtg cagacggggc gtactaccgc gcgcccggtc aaccgacgcg ccacgtcccg
     3841 gccatcccgg tggacgtggt agacacggta ggcgccgggg acgcgttcca cggcgggctg
     3901 ctctacgccc tttcccgcgg ggaaaccctc gcggatgcgg tgcttctggg aacgcgctgc
     3961 ggggccgccg cctgcctcaa gttcggcgcg cgca
//
LOCUS       DAOSOO010000262         3931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23158_length_3931_cov_3.545666, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000262 DAOSOO010000000
VERSION     DAOSOO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23158_length_3931_cov_3.545666"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLT86_13450"
     CDS             <1..194
                     /locus_tag="PLT86_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT37662.1"
                     /translation="FVRCDGTILGSLRFHRAGVMVNEVDTTTQFYDASHAYRDRAMAG
                     ILSSADGVDDPRSRDRRSL"
     gene            579..1700
                     /locus_tag="PLT86_13455"
     CDS             579..1700
                     /locus_tag="PLT86_13455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37663.1"
                     /translation="MKRSIRSLTAGGLAVLCLLARGVPAKTDSLTTEQRIQRVFPFQV
                     GQQYEFQSGVTFHRPNGQALPPYTIAQITVTDTVINGKTYLHIPYWSSFGTEYYRLDD
                     SLRIWNYSPDSSKERVLLNLGRNRKDLTIGDGVYCALPCAEYNYYCCLDVVSTGNRPL
                     FSGDSVHIDNPFWWAYYNGDKAGMANVVRKARVKTGWTVAGGTAESDTVLGYTFDVGS
                     APSPEISELYGIGGPGASRHRQGIQTATFEMNKVSIGVFRQKSSEPWPAIRDWTPTAV
                     APPTLSARYAVEAFPNPFNATTLLRYSVPTAGHVTMAIYSIEGRLVKTLVDREVAAGT
                     HETEWEGRGNDGNEVGSGVYVVRVVASNSVLLKPVTLLR"
     gene            1741..>3931
                     /locus_tag="PLT86_13460"
     CDS             1741..>3931
                     /locus_tag="PLT86_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37664.1"
                     /translation="MNTLRSAFLFAACLCAGSYAQAPQEGAVSERQRASKLLQMVIGT
                     ENFARLSEAEREDLLSGRVFCATDMLRRTERNRSASRPAITQGITRVGDSFFAGKKYR
                     LILIYITPASGDTAKPLLVRNSFLPGRVPNGGSSTRNWTQSDTLDLAMYTKEAIQYLI
                     GQYTYGELDTSDFRIDIPVPNGVTPIWRSQGDSGTAVVDSVLRRIEDTYPPGDPGADY
                     FALPAGYDYQRVGYVFVDKYDGLDTNAFAMSSEDNETWTGSVGQAIWNGATFAAATQW
                     SGTSCGMWADRHSETGVGIIFHELLHHLIYACSENPLLGDRGIWDKHTRTWGFDVMDH
                     NGGKPEPTNGHYGVPPLSPIDQYMLGFIKSAWYAEITNNSTDVRIRPRTTVLSPSSTD
                     KLLYRIPMNEANVESFVISNHQGTGIDEVYNEGLGDTLTRGLQIWHISPLFRLAGGTA
                     TADVEVAWTLALGDTFRHPDGSRTWLVGDYLDSTHYHENLDWVDCSNLTDSPLYPAPA
                     GKSLHIIQGAWGGYSCGTPGDSHGYYNNENGRSTWQFDTFTGNPTRNDFFTACKEFTP
                     YTAPSTDTYHLYRADNVVGAADDCSGLRQLDPPREGALYTNSSPTGTHIALVNIRQEA
                     GGDMLFDVYFNFWEGNFSHAKNFGAASTGYWGQEYDNTDTVVVGPNGFVVDSSRTLTI
                     RSATTVRCLGGITVHGTLTVQSGVFFDTIKGDIVIASGGRLELGSNVT"
BASE COUNT          905 a         1065 c         1114 g          847 t
ORIGIN      
        1 tgttcgtccg gtgcgacgga acaatcctcg gctcgctgcg tttccaccgc gccggcgtca
       61 tggtgaacga agtcgacacc acaacgcagt tctacgacgc atcccatgcg taccgcgacc
      121 gggcgatggc gggcatcctc tcaagcgcgg acggcgtgga cgatccccgt tcgcgcgaca
      181 ggaggagtct ctaggggtat tgtcggcacg cgtgcgacgc cgacggccct cgcaaacgca
      241 caaaaccatg atgatcaaca tgttatgaga ctgaggaaaa aactccgcgc gcatgcgctc
      301 cggggcttga caggaaaccg atctttctgt aaaaatctgt tgcatatttt agttggtaac
      361 cccaattgca tggttaccct tgacgagagg agccgaacca tgcctttctg catttctact
      421 ctcccgcctc cggggtgtat ccgggggggg ggtacaggct cccgcccgcc gctgaaatgc
      481 ccacgagcac gccaccatcc cgaacgacca ccgtgaaaaa ccttgaaccg aaaccatgcc
      541 tcctgagagg catttgtgcg ttctgaggag aagcaaccat gaaaagaagt atccggtctc
      601 tcacggcggg cgggcttgcg gttctttgcc tgcttgcgcg cggggtaccc gcaaaaacgg
      661 atagcctcac cacggaacag cgcattcagc gagtgtttcc gttccaggtt ggccaacaat
      721 acgagtttca gtccggggtt acgttccatc gccccaacgg ccaagccctt cctccctaca
      781 ccatcgccca gattacggtc accgacacgg ttatcaacgg aaaaacttat ctgcatatcc
      841 cctattggtc gagtttcggc accgagtatt accggctcga cgactcgctg cggatatgga
      901 attacagtcc cgacagtagc aaggaaagag ttctgctcaa tttggggagg aacaggaagg
      961 acctaacgat cggtgacgga gtctattgcg cactgccttg cgccgaatac aactattact
     1021 gctgtctgga tgtcgtgtcg accggaaacc gcccattgtt cagcggggat tccgtacaca
     1081 ttgataaccc gttctggtgg gcatattaca acggggacaa ggctggcatg gccaatgtag
     1141 ttcgcaaagc tcgtgtgaag acgggctgga cagtcgctgg cggaacggct gaaagcgata
     1201 cggtacttgg gtacacgttc gacgtaggtt cggcaccatc gccggagatt tccgagttgt
     1261 acggcatcgg agggccgggc gcgagcaggc ataggcaggg tatccaaacg gcaacctttg
     1321 agatgaacaa ggtctcaatc ggtgtgtttc gtcagaagag ctccgagcca tggcctgcga
     1381 ttcgggattg gaccccgacg gcggtagccc cccccactct gtctgcgcgg tatgccgtgg
     1441 aggctttccc caacccattc aacgccacga cgcttctgcg ttactccgtg ccgacggcgg
     1501 gacacgtcac aatggcgatt tactcaatcg aaggccggtt agtgaagacg ctcgttgaca
     1561 gggaggtggc ggcgggtacg catgaaaccg aatgggaggg ccgcggaaat gacggaaatg
     1621 aagtagggag cggagtatat gttgtccgcg ttgtggcgtc gaactcggtg ttattgaagc
     1681 cggtgacact tctacgatag tcgttcctcg tgatctctct tgcggatccg ggaggttttt
     1741 gtgaacaccc tgagatcggc ttttctgttc gcggcctgtc tttgcgccgg gagttacgca
     1801 caagcgccac aagaaggtgc ggtttccgag cgtcagcgcg cgagcaagtt gctccaaatg
     1861 gttatcggaa cagagaactt cgctcgcttg agcgaggcag aacgggaaga tctattgagc
     1921 ggtcgagttt tctgcgctac cgatatgctc agacgtacgg aaagaaatcg cagcgcatct
     1981 cgccccgcta tcactcaggg aatcacgagg gtcggggaca gctttttcgc gggcaagaag
     2041 taccgactga tactcatcta tattacgccc gccagcggag ataccgcgaa gccgctactt
     2101 gtacgcaatt cattcctccc tgggcgagtt cccaacggag gctcatcaac acgcaactgg
     2161 actcaatccg atacacttga tctggcgatg tacaccaaag aagcaatcca atacctaatc
     2221 ggtcagtata cctacgggga gcttgatacg agcgattttc ggatcgatat cccggttcca
     2281 aatggcgtga ccccaatatg gagatcccaa ggagattcgg gtaccgccgt ggtagacagt
     2341 gtcctacgac gtatcgagga cacgtaccct ccgggtgatc ccggtgcgga ttatttcgcg
     2401 ctaccggctg gttacgacta tcagagagta ggctacgttt ttgtggacaa atacgacggc
     2461 ttagacacca acgctttcgc gatgtcgtct gaagacaatg aaacgtggac gggttcggtg
     2521 ggtcaggcga tttggaatgg cgccaccttc gccgcggcca ctcaatggtc gggaacctct
     2581 tgcggcatgt gggccgatcg ccactcagag acgggggtgg gcatcatctt tcatgaattg
     2641 ctccatcatt tgatttacgc ttgttccgaa aatccactac ttggcgatcg cgggatatgg
     2701 gacaaacata ccagaacttg ggggtttgac gtcatggatc acaacggcgg caaaccagaa
     2761 cccacgaacg ggcattacgg agtgccacct ctgtctccaa tagaccagta catgctgggg
     2821 ttcataaaaa gtgcttggta tgcggaaata acgaacaaca gcacggacgt gcggattaga
     2881 ccgcggacga ccgttctttc gccgtcatct accgacaaac tgctttacag aataccaatg
     2941 aacgaggcaa acgtggagtc cttcgtcatt tcgaatcatc agggcacggg gatcgatgag
     3001 gtttacaatg aagggctagg cgatacgctc accaggggac tccagatttg gcatatctcc
     3061 ccgttgttcc gattggccgg cggtaccgcc accgctgatg tcgaggttgc gtggacgctt
     3121 gcgctaggag atactttccg acatcctgat ggaagcagaa catggttggt tggtgattac
     3181 ttggattcca ctcactacca tgaaaatctg gactgggtag attgctcgaa tctgacggat
     3241 tcgcctttat atccagcacc tgcaggcaaa tcattgcaca ttatccaagg cgcctggggg
     3301 ggatacagct gtggcacgcc tggagacagc catgggtact acaacaacga gaatggaagg
     3361 agtacatggc agtttgacac atttactggc aatcccacac gcaacgactt tttcactgca
     3421 tgcaaggaat tcacgccgta cactgccccc agcaccgaca catatcatct gtacagagca
     3481 gacaacgttg tcggggctgc agacgactgc tccgggctga ggcaattgga tccccctcgc
     3541 gaaggggcgt tgtacactaa ctcaagcccg accggtacgc atatcgcgct ggtcaatatc
     3601 cgccaagagg caggcggcga catgctcttt gatgtctatt tcaatttctg ggaaggcaac
     3661 ttttcccacg cgaagaattt tggcgccgcc tctaccgggt attggggaca ggaatacgac
     3721 aacaccgaca ccgtagttgt ggggccaaac ggcttcgtgg tagacagttc gaggactctc
     3781 acgatccgtt ctgctactac tgtccgctgt cttggaggga taacggttca cggcactttg
     3841 acggtccagt caggcgtgtt tttcgacacg atcaagggcg acattgtcat cgccagcggc
     3901 gggcggcttg agttgggatc gaatgtcacc c
//
LOCUS       DAOSOO010000263         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23237_length_3919_cov_6.063923, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000263 DAOSOO010000000
VERSION     DAOSOO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23237_length_3919_cov_6.063923"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..>2223)
                     /locus_tag="PLT86_13465"
     CDS             complement(201..>2223)
                     /locus_tag="PLT86_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT37665.1"
                     /translation="LSGTREETRLSTAITSSRLRTGCPVLRDSLRTGGLRVARGFVAA
                     LSIGFLSSPSPAAEADSTISVWVFLTDHGESRGVLNRPSAPVSDRARARMATRGSRTE
                     DVLAVYAPYVERVSSLVNRVRTVSRWLNAVSADATPAQIESLRALPEVRAVSPVRTYR
                     HELPDIDATQARMAPGLADTVQQRYGASFWQYGRVGIPELHALGLTGSGVMICVIDAG
                     FTRFDHPAFAHLKVVQTKDFITGSTSLTPDSHGAQVLSVIAGNEAGMLVGPAYGAEYT
                     LARTEARAVEVRAEEDFWIAAVEWADSAGVDIITSSLGYNTFDDPSENHSLAELDGSS
                     LITRAADRAVARGIVVVASAGNERGASGAPEWQGRITLPADGFGVLAVGSLTAADQIA
                     ASSSTGPTADGRIKPDLVAPGDNVLALDVAMTSGYARVSGTSFAAPIVAGAAALLLEK
                     NPLWTPADVRRALLWSAEDILAPGPDVFSGWGVMNANLAVDEVVHSGIVGRVYSTVAY
                     VGGQTKDVPVAGVRVELRNGNGVLVAETTTDARGRFSFSGHPDGAYRVRLSHPGFDET
                     QSDVTIPQTVQLRFTLLPASSAGAQAYRLFPNPATPASGVTISGAAHPDLIAKFYDIS
                     GTLVRTLGPQQMYWDLTSQGGRQVAGGVYFCRIEVAGSEVWNGKIAVVR"
     assembly_gap    2224..2323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2324..3219)
                     /gene="proB"
                     /locus_tag="PLT86_13470"
     CDS             complement(<2324..3219)
                     /gene="proB"
                     /locus_tag="PLT86_13470"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT37666.1"
                     /translation="MSEAISQTRQRAYDLDRSFDRRTALGNVRRVVVKVGTSTLSSEN
                     GRLDRAYIAELMEQVAGLRNSGREVAIVSSGAVGAGIGALNLKKRPGSIGDLQAVAAV
                     GQSLLMHFYDDAASPMGIRVAQVLLSAADLRRKVGYQNVRSVFESLFRLKALPIVNEN
                     DSVAVEELKFGDNDSLSAHVANLVDADLLVILSDVDGLYRGTPGAESAPPLIPTVYRI
                     ESEVERLCGASTSGVGIGGMATKISAAKMLAASGVPTIIANGRTTRLEDIVSGKPVGT
                     IFLPVSVSAHTSRHQHWILAQKT"
     gene            complement(3216..>3919)
                     /gene="aroF"
                     /locus_tag="PLT86_13475"
     CDS             complement(3216..>3919)
                     /gene="aroF"
                     /locus_tag="PLT86_13475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOT37667.1"
                     /translation="ENEEQLLSTARKVKAAGARVLRGGAFKPRTSPYAFQGLAEEGLR
                     LLSLARSETGLPFATEVVTNRDVELIARYADMLQIGARNMTNYSLLREVGATGKPVLL
                     KRGLSATLEELLLAAEYIADEGNEQIVLCERGIRTFEPFTRNTLDLSAVPAIHMLSHL
                     PIIVDPSHATGRSELVRSMALAAIAAEADGLIVEVHPAPEQALSDGAQSILPEAFAQL
                     MEEGRRYLAVSGRTL"
BASE COUNT          732 a         1211 c         1136 g          740 t
ORIGIN      
        1 gaccgtttgt gtacgggaaa aggttcatga tagtcatcgt gcagctcatc cgtcccacaa
       61 caagatgcgc cgcgtcgtcg gtggtgtgcg cggcctcgca gaaaacacat ccttccgacc
      121 tgcccgcttc gccaaggatg tatttcattc gccaaggagc ccagaggcgt tccataggtt
      181 tacctctcat tcccgccgca ttaccggacg acagcgattt tcccgttcca cacctcagaa
      241 cctgccactt ctatcctgca gaaatacacg cctcccgcta cttgtcttcc gccctgcgaa
      301 gtgaggtccc agtacatctg ttggggcccg agcgttcgca ccagcgtacc ggaaatatcg
      361 tagaatttcg cgatgaggtc agggtgggcc gcgccggaaa tcgtaacgcc gctcgcgggc
      421 gtcgcgggat tcgggaacaa acggtacgcc tgggcgccgg ccgacgaggc agggagaagc
      481 gtgaagcgta actgcacggt ttgtgggatt gtgacgtccg attgagtctc gtcgaaccct
      541 ggatgggaga gacgaaccct gtacgcgcca tcagggtggc cgctgaaact gaaccttccc
      601 cgtgcgtccg tagttgtttc agccaccaac acgccatttc cgtttcgcag ctccacacgc
      661 acgcctgcga ccggcacatc tttcgtctgc ccaccgacgt acgcgacggt cgaatatacc
      721 ctccctacga tacccgagtg aacaacttca tctactgcca ggttggcatt catcactccc
      781 cacccggaaa aaacatccgg ccccggcgca agaatgtctt ccgccgacca gaggagcgcg
      841 cggcgcacgt ccgcgggcgt ccagagcgga ttcttctcca gcagcaacgc cgccgcgccg
      901 gcgacgatcg gtgccgcgaa ggatgtgccc gaaacgcgcg catagcccga cgtcatcgca
      961 acgtcgagcg ccagcacgtt gtcacccggt gccaccagat ccggtttgat tcttccgtcg
     1021 gccgttggtc cggttgaact cgatgccgcg atctggtcgg ccgcggtgag ggaaccgaca
     1081 gccagaaccc cgaaaccgtc cgcgggaagg gtaatccgcc cctgccattc cggcgccccg
     1141 ctcgcaccgc gttcatttcc cgccgaagcc acgacgacaa tcccgcgcgc cacagcacga
     1201 tcagcggccc gggtgatcaa cgaagagccg tccagttccg cgagactgtg gttctcggac
     1261 ggatcgtcga acgtgttgta cccgaggctg ctcgtgatga tgtccacacc ggcggagtcc
     1321 gcccattcca ctgccgcgat ccagaaatcc tcttcggcgc gaacttccac tgctcgggct
     1381 tccgtacggg caagcgtgta ctccgcaccg tacgccggac cgaccagcat tcccgcttcg
     1441 ttcccggcaa tcaccgacag aacctgcgcc ccgtgtgaat cgggcgtcaa actggtagaa
     1501 ccggtgatga agtccttcgt ctggacgact ttcaggtgag caaacgcggg gtgatcaaac
     1561 cgggtaaatc cagcgtcaat cacgcaaatc atgaccccgc ttccggtaag gccgagcgcg
     1621 tgcagctccg gaatcccgac gcggccgtac tgccagaaac tggctccgta ccgttgctga
     1681 accgtgtccg caagcccggg tgccatgcgt gcctgtgtgg cgtcgatgtc gggcagctcg
     1741 tgccggtacg tacgtaccgg gctgacagcg cgtacctcgg gcagcgcgcg aaggctctca
     1801 atctgtgccg gtgttgcgtc cgcgcttacc gcattcagcc agcgggagac ggtgcgcacg
     1861 cggttgacga gcgatgagac ccgttcgaca tacggggcat acacagccag aacgtcttcc
     1921 gtccgcgaac cgcgagtggc cattcgtgct cttgcgcggt cggaaacagg cgccgaagga
     1981 cggttgagca cgccgcgcga ttcaccgtgg tcggtgagaa aaacccacac ggaaatggtg
     2041 gaatctgctt ctgccgcggg tgaaggggag gaaaggaacc cgatggaaag cgccgcaaca
     2101 aagcctcgcg cgacgcgaag gcccccggta cgcagactat cgcgcagcac ggggcaaccc
     2161 gtccgtaacc gtgacgaggt tatcgcggtg gacaacctcg tctcctcgcg tgtacccgag
     2221 agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttttct gggcgaggat
     2341 ccagtgctgg tgcctgctgg tgtgtgcaga gacagagacg ggcaggaaga tggttcccac
     2401 tggcttgccg gatacgatgt cttccaggcg cgttgtacga ccattggcaa tgatcgtcgg
     2461 tactcctgat gcggccagca ttttggcggc gctgattttg gttgccattc ccccgattcc
     2521 cacgccgctc gtgctggcac cacagagcct ttccacctcg gattcgattc gatagaccgt
     2581 ggggatcagc ggaggagccg attcggcacc cggggttccc cggtacaatc cgtccacatc
     2641 tgacagtatc acgagaaggt cagcgtccac gagatttgcc acatgcgccg aaagagagtc
     2701 gttgtccccg aatttcagtt cctcaacggc aacggaatcg ttctcattca cgatggggag
     2761 ggctttcaag cggaagagcg attcaaacac gctgcggaca ttctggtacc cgactttacg
     2821 gcgcagatcg gcggcgctca ggagtacctg cgcgacgcgg atccccatcg gcgatgccgc
     2881 atcgtcataa aagtgcatga ggagcgattg gcccactgcc gccacagcct gaaggtcacc
     2941 gattgaaccc ggtcgctttt tgaggttgag agctccgatt cccgcgccaa ctgcgccgct
     3001 ggatacgatc gcgacttcgc ggccgctgtt cctcagtccg gcgacctgtt ccatcaattc
     3061 tgcgatgtaa gctctgtcga ggcgtccatt ttcggacgag agcgtgctgg ttccgacctt
     3121 taccaccacg cgcctgacgt tgcctaacgc ggtgcgccgg tcaaacgagc ggtccaggtc
     3181 atacgcccgt tgccttgtct gcgagatcgc ttcgctcata acgtgcgccc ggatacggca
     3241 aggtagcgcc gcccctcttc catgagttgc gcgaacgcct cgggaaggat cgattgcgca
     3301 ccgtcgctca gcgcctgttc cggagccggg tggacctcca cgataagccc gtcggcttcg
     3361 gcggcgatcg ccgcaagggc cattgaacga acgagttcgc tccgtccggt tgcatggctt
     3421 ggatcaacta tgatcggcag atgggacaac atatggatcg ccggtaccgc cgaaaggtcc
     3481 agcgtatttc gggtgaaagg ttcaaaggtg cggatgccgc gttcgcacag cacgatctgc
     3541 tcgttgccct cgtcagcgat gtattccgcc gcgagcagca gttcttcaag tgtcgccgac
     3601 aatccccgct taagaagaac tggttttccg gtggcgccaa cttcgcggag gagcgagtag
     3661 ttggtcatgt tccgcgcgcc gatctgcagc atgtctgcgt aacgggcgat cagttccacg
     3721 tcccggttgg tcactacctc ggtcgcaaac ggaagtccag tctcgcttct tgcgagggaa
     3781 aggagcctga gcccttcctc tgcgaggccc tggaacgcgt agggcgaggt tcggggtttg
     3841 aatgcgccgc cgcgaagcac tcgtgcgccc gctgctttca ctttccgcgc ggtggaaagg
     3901 agctgttcct cgttttcta
//
LOCUS       DAOSOO010000264         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23254_length_3917_cov_6.291041, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000264 DAOSOO010000000
VERSION     DAOSOO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23254_length_3917_cov_6.291041"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLT86_13480"
     CDS             complement(<1..182)
                     /locus_tag="PLT86_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37668.1"
                     /translation="MPQPDAGGVLSTLPTQSTETGERTPHDAIRNAHRWSTTGSTGRR
                     RAGGFALAAAETIFSIT"
     gene            399..1535
                     /locus_tag="PLT86_13485"
     CDS             399..1535
                     /locus_tag="PLT86_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT37669.1"
                     /translation="MDGRYLFMLTGKAALYALAAFVGFVAAGLLVAVGLVWMNSSGRP
                     KPFLDSRGNPLAGSISEKIFVNINGTKQGMFIKGKRRNAPVLLYLHGGMPDYFLTDKY
                     PTGLDELFTVVWWEQRGSGISYDSGLDRSAMTVDTMMNDVITVADYLRQRFRQDRIYL
                     MGHSGGTFLGIRVAATHPELFRAYIGVSQISRQKVSEKLAYDYMVRRFAAEGNADMVG
                     KLRESPVTLTGQIPDSYLKIRDKAMHELGIGTMRNMRNVFTGLFIPSLLFDEYSLGEK
                     YRLWAGKSHAGVSVIWNDIISTDLSEEITSLDIPVYFLHGRYDYTCSFALAEEFFRKI
                     EAPVKGFYVFEKSAHCPLFEEPLKTVGIIRDDILRGRDDHADVK"
     assembly_gap    1751..1850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1851..2596)
                     /locus_tag="PLT86_13490"
     CDS             complement(<1851..2596)
                     /locus_tag="PLT86_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT37670.1"
                     /translation="MAGTQENTVRARAVRKRFPMGEGYLEALKGIDLEIKRGEYISIM
                     GPSGSGKTTLFNMIGGLDKPSDGTVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLI
                     PVMTALENVTLPMIFAGLSNDEAISKGRGLLDTVGLGERWQHKPTELSGGQQQRVAIA
                     RSFANDPSIILADEPTANLDLKTGHDIIELLRKLNKEMGVTIISNTHDPSMLNVSDRI
                     IWIRDGRVDRIEARSDIDINVGTIGGDVLGG"
     gene            complement(2637..3323)
                     /locus_tag="PLT86_13495"
     CDS             complement(2637..3323)
                     /locus_tag="PLT86_13495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT37671.1"
                     /translation="MDEQITHEEAIRRLRTDMKTSSVATPFATALKIAFQSLRIRFWR
                     SIITIAGIFFAIAFLVSVLNSAVVELAVNPDEGSQVSGTGIRFILTLLAAEPRQAWLV
                     TMSLIVCVIGIANAMLMSVTERYKEIGTMKCLGALDIFVIELFLLESGFQGLVGSFTG
                     ALIGAALVLVSALVKHGFAVMGILPWSQMVINILLATVIGLLLTLLGASFPAYRASQM
                     PPADAMRTEV"
     gene            complement(3352..>3917)
                     /locus_tag="PLT86_13500"
     CDS             complement(3352..>3917)
                     /locus_tag="PLT86_13500"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT37672.1"
                     /translation="TVERACSHAFPRRRVGRLVFVRTDTALRAQPFSRYSYLLIIHRL
                     HQQESLPGSMFGKLFKKREAPRLTKDDLLSSRPVLNQAISWSLNNQQEVQITIPRREV
                     WWATLLSKIIYTPKERVIALDEVGTAVWKMIERRLTVREMIAELSAQYNLNRREAEAS
                     LIEYLRRLAKKNFVGFEVPRQRRKTKR"
BASE COUNT          785 a         1097 c         1133 g          802 t
ORIGIN      
        1 gtgattgaga agatcgtttc cgcggcggcg agggcgaacc cacccgcgcg ccggcgcccg
       61 gtgctgccgg ttgtgctcca ccgatgggcg ttgcggatag cgtcatgtgg cgtgcgttct
      121 ccggtttcgg tggactgcgt gggcagcgtg gacaggacgc cccctgcatc tggctgtggc
      181 aaagaggcgt cttcgtgtca agagggggag cgaaaaaaag ttcagcgggc ggtggagggg
      241 cacgctgcaa cgcctccgcg tgccgacagg agcccacggt ttgcctgtcg aaggtgctcg
      301 tgctacgtta ctcctgatga tgcggccgat ttggacaggg ccttggcagt gcgggtgcac
      361 ccgattctcg aatgcggttc cgtgggagta ctccagaaat ggacgggaga tatctcttca
      421 tgctgacggg aaaagcggcg ctctatgcgc tcgccgcctt tgtgggtttt gttgcagccg
      481 ggttgctggt ggcggtgggg ctggtgtgga tgaacagctc cggcaggcca aaacccttct
      541 tggattcccg cggcaatccg cttgccggga gcatctccga gaaaatcttc gtgaacatca
      601 atggcacgaa gcagggaatg ttcatcaagg gaaaacgcag gaacgccccc gtgttgctgt
      661 acctgcatgg cggcatgcca gactatttcc tcacggacaa atatccgacg ggactcgatg
      721 agctgttcac cgtggtctgg tgggaacagc gagggtcggg gatttcctac gattccggtc
      781 tcgatcgttc cgcgatgacc gtggacacca tgatgaacga tgtcatcacg gttgcagatt
      841 acctcagaca gagattccgt caggacagga tctatttgat gggacattct ggtgggacat
      901 ttcttggaat cagggtagcg gcaacacatc cggagttgtt ccgggcttac atcggggtct
      961 ctcagatatc tcgccagaag gtttccgaga aactcgcgta cgattacatg gtgcggcgat
     1021 tcgcggcaga aggaaacgcc gacatggtgg gaaaactcag ggagtcaccc gtcaccctga
     1081 ccgggcaaat acccgactcc tatctcaaga tccgcgacaa agccatgcat gaactcggca
     1141 tcggaacaat gcgtaacatg cgtaacgtgt tcaccggtct gttcattccg tcgttgctgt
     1201 tcgatgagta ttcgcttggg gagaagtaca ggctgtgggc gggcaaatcg cacgcaggtg
     1261 taagcgttat ctggaatgac atcatttcca cggacctgtc tgaagagatc acgtcactcg
     1321 atatccctgt ctacttcctc cacgggagat acgattacac ctgctcgttt gcgctggcag
     1381 aggagttctt cagaaaaatc gaggcaccgg tcaagggatt ttacgtgttc gagaagtcgg
     1441 cgcattgtcc gctatttgag gaaccactga aaaccgtggg aattatcaga gatgatatct
     1501 tacggggaag ggacgatcat gcggacgtga aatagcactc attcggcacc cgcccacgcg
     1561 aaatcgggaa cctcccccag ggtcacgacg aagcgtgacc ctccaccccg tcacgccgga
     1621 tccccgcctg cgcgcctcac gttaaggaag acacatgtac ggtttttcgc ggcggaacag
     1681 ggtcggcatg cgaccggagg aagagcgcgg ggtcattgtg gttcctcccg tacacacgca
     1741 gagccgctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgccgagca
     1861 cgtcgccacc gatggttccg acattgatgt cgatatccga gcgggcttct atgcggtcca
     1921 cgcgcccgtc gcgaatccag atgatgcggt cggagacgtt caacatgctg gggtcgtgcg
     1981 tgttggagat gatggtgacg cccatctcct tgttgagctt gcggaggagt tcgatgatgt
     2041 cgtgcccggt tttcaggtcg aggtttgccg tcggttcgtc cgccagaatg atggagggat
     2101 cattcgcgaa ggaccgcgca atcgcaacgc gctgctgctg cccgccggaa agctccgtcg
     2161 gcttgtgctg ccaccgttcc ccgaggccca cggtatccag caggccgcgc cccttcgaga
     2221 tcgcctcatc gtttgaaagc ccggcgaaaa tcatcggcag ggtgacgttt tccagcgccg
     2281 tcatcacggg gatgaggttg aatgtctgga aaatgtagcc gatcttccgg caacggagcc
     2341 atgcgagctc gtacgcgtcc aactgggcta tatccacctc gtcgatgtac accgttccat
     2401 cagagggttt gtccagaccg ccgatcatgt tgaaaagcgt ggttttgccg gagccggacg
     2461 gacccatgat gctgatgtac tcgccgcgct tgatttccag atcgatgccc ttcagcgcct
     2521 ccagatagcc ctcacccatc gggaaccgtt tccggacggc gcgcgcgcga acggtgtttt
     2581 cctgtgtacc tgccatcgag ctaacccctc attcgagatg aagcagtgaa tatctgtcag
     2641 acttccgtgc gcattgcgtc tgccggtggc atctgggatg cgcggtatgc gggaaaactg
     2701 gcacccagca gcgttaacaa aagcccgatg accgttgcca gcaggatgtt gatcaccatc
     2761 tgcgaccagg gcagaattcc cataacggcg aacccgtgtt tgaccagcgc actcaccagc
     2821 accagcgccg caccgatcag cgcgccggta aacgacccta ccagcccctg gaacccggat
     2881 tcgagcaaaa acagctcaat cacgaatatg tccagcgctc cgaggcactt catcgttccg
     2941 atttccttgt agcgctcggt taccgacatc agcatcgcgt tggcaattcc gatgacgcat
     3001 acgatcagcg acatggtaac cagccatgcc tgacgtggct ccgcggcaag cagggtcaga
     3061 ataaacctga ttccggttcc agatacctgc gagccctcgt ccggattcac ggccagttcg
     3121 acaaccgcgg aattcaggac ggagaccagg aaggcgatgg cgaaaaagat gcccgcgata
     3181 gtaatgatcg accgccagaa ccttatgcgg aggctctgaa acgcgatttt cagcgccgtg
     3241 gcgaacggcg ttgccacgct ggaagtcttc atgtcggtgc gcagacgccg gattgcttct
     3301 tcgtgggtga tctgttcatc catgggtgcc tcgtttcaag ccttccgccc gttatcgctt
     3361 cgttttccgc cgctgccgcg gcacttcaaa accgacgaag ttcttcttcg ccagcctccg
     3421 gagatactcg atcagcgacg cttccgcctc ccggcggttg aggttgtact gcgcgctgag
     3481 ttcggcaatc atctcccgca ccgtgaggcg gcgctcgatc atcttccaca cggccgtgcc
     3541 cacctcatcc agcgcgataa cccgctcctt cggggtgtag atgatcttgg aaagcagcgt
     3601 tgcccaccac acttcgcgac gcgggattgt aatctgcact tcctgctgat tgttcagcga
     3661 ccagctgatc gcctgattca gcaccggccg tgagctgagc aggtcatcct tagtgagccg
     3721 gggcgcttcc cgctttttga acagctttcc gaacatactc cccgggagac tttcctgctg
     3781 atggagacgg tggattatga gaaggtaaga ataacgggaa aaaggttgcg cgcgcaaggc
     3841 ggtgtctgtg cgaacgaaaa cgagccgccc cacccgtcgg cggggaaacg cgtgggaaca
     3901 cgcacgctcc accgttc
//
LOCUS       DAOSOO010000265         3913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23294_length_3913_cov_4.515293, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000265 DAOSOO010000000
VERSION     DAOSOO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23294_length_3913_cov_4.515293"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..756
                     /locus_tag="PLT86_13505"
     CDS             16..756
                     /locus_tag="PLT86_13505"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOT37673.1"
                     /translation="MNTDELERLRAEVTAALTRRDAPPPAGTGAGSGPAPPRLVTEEC
                     VLAARAKGEAVIRVAPGAIITPLARDALARFGIAVASALGSRGASGGKPAPDAESVRA
                     ARTARVAVGVAATSAVEPLLVEALRAAGQAVVRVPGSARKGALLAKRVAGAVASGQAD
                     WGVVVDETGLVASAVANRVKGVRAACCNDVAAAKAARERMAANVLCMGADIVAPALMR
                     EVLTIWLTTEPQISPEIAAILDGMGREA"
     gene            753..1508
                     /gene="deoC"
                     /locus_tag="PLT86_13510"
     CDS             753..1508
                     /gene="deoC"
                     /locus_tag="PLT86_13510"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT37674.1"
                     /translation="MINQMVDSVVAAVMRRLAAEEGASEAAPSPAEGDAAVDLAPLID
                     HTLLRPDATPEDVDRLCDEAIRHHFAAVCVHPRFAAQAVERVRMTQVAVAAVVGFPHG
                     ATLPETKRAEAEALVARGVHELDMVLPVGILKANLWSEVAADIDGVVAAARQRSGTPA
                     LVKVIIEAPLLTDREKAIACIIAEQCGADFVKTCTGFSGAATVEDVALMRRVVGDRLG
                     VKAAGGIRTREQALAFVRAGATRIGTSRGPALV"
     gene            1612..2109
                     /locus_tag="PLT86_13515"
     CDS             1612..2109
                     /locus_tag="PLT86_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37675.1"
                     /translation="MIRTLREYADGPDLGVGRPLRQKTELLGVSRAQIWFPETGEVNS
                     ALREKRVEISYANKRVAKHFADRKELERRYGKPCAVAIMRRITDLQAAPNLAALSFSE
                     DPSARCHELKHDLKGYLAMDVSKSRRLLFAPGNEPIPRKSDGGLDWVRVTCVVIHRVE
                     DYHNG"
     gene            2102..2449
                     /locus_tag="PLT86_13520"
     CDS             2102..2449
                     /locus_tag="PLT86_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37676.1"
                     /translation="MAKVAMSAFPPNWVVTPGELVQDHLNGAGITRSSFVEKTGLAAE
                     TLDAVIADEIRITEDIAEALATALGLPVSFWVNLQAHFDSEAKRLKIPLKYVSAGQSH
                     RNTGAAAGTRVPA"
     gene            2633..>3913
                     /locus_tag="PLT86_13525"
     CDS             2633..>3913
                     /locus_tag="PLT86_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37677.1"
                     /translation="MTNLLSNGGFEDAADGIPVGWTYSGPAARQDETWPEGGAHGGVR
                     CVRFAARTDRQAWTSERVPVQGGMPFRLEWWTLLAGSIPWHWTTHTGFIGVRVTFFDA
                     AGDRCGITERRIRSIGTDGWVRCWLDVNPAPEASSCTVSLVCEAEIETDGTVAFDDVA
                     LVPAGMTRTPAGRGHLRGVVWTAEDPAPGFARVLITAADGRHYAPENSYQFLDGSFHA
                     TGEPFELALPAGRAAVLVTRGFEYSIWRDTVDIRAGQRTEVAPRLAHRWPLSAEGWYS
                     GDAHIHLFFHRHSLHPQMVPADVFALARAEGLGWLSFKGEEKEAREYIARSAPLCVPG
                     FTGEIGIEAVSDFHGHVYTLNARTIPADGFPMKLVPWPMNLDLGAALDSVGGALVYAH
                     PSGDLAADGVLEGIGDPSRLLLAREWPVDLALGQN"
BASE COUNT          642 a         1107 c         1464 g          700 t
ORIGIN      
        1 agcttttagg cagcgatgaa tacggacgaa ctggaacgcc tcagagccga agtgacggcg
       61 gcactcaccc ggcgtgatgc accaccgccg gcgggcacgg gagcgggctc cggtcccgct
      121 cctccgcggc tggtgacgga ggagtgcgtt cttgccgccc gtgcgaaagg cgaggcggtg
      181 atccgggtcg cgcccggcgc catcatcacc ccgctggcga gggatgcgct ggcccggttc
      241 gggattgccg tggcgtccgc gctgggctca cgcggtgcgt ccggtggaaa accggcgccg
      301 gatgccgagt ccgtgcgtgc tgccaggacg gcgcgtgttg ccgtcggggt ggcggcgacc
      361 tccgcggtgg agccgctgct ggtggaggca ttgcgcgcgg cggggcaggc ggtggtacgg
      421 gttcccggct cggcgcggaa gggggcgctg ctcgccaaac gagtcgccgg cgcggtggcc
      481 tccgggcagg cggactgggg cgtggtggtg gatgagaccg gtctggttgc ctccgccgtg
      541 gccaatcggg tgaagggcgt gcgggctgcc tgctgcaatg atgtggccgc agcgaaggcg
      601 gcacgggagc gaatggcggc aaatgtcctc tgcatgggcg cggacatcgt tgcgccggct
      661 ctcatgcggg aggtgttaac catctggttg actacggaac cgcagatttc gcccgaaatc
      721 gccgcaatcc tcgacgggat gggccgtgag gcatgattaa ccagatggtt gactcggttg
      781 ttgccgcggt gatgcggcgg ctggcagcgg aggaaggggc atctgaggcg gcgccttcgc
      841 ccgcggaggg ggacgccgcg gtggatctcg ccccgctgat tgaccacacg ctgctccgcc
      901 ccgatgcgac gccggaggac gttgaccgcc tctgcgacga agccatccgg caccatttcg
      961 ccgcggtgtg cgtgcatcca cggttcgccg cgcaggcagt ggagcgggtg cggatgacgc
     1021 aggtggccgt tgcggcggtg gtcgggttcc cccacggcgc gacattgccg gagacaaagc
     1081 gcgcggaagc cgaagcactg gtggcgcgag gggtgcacga actcgacatg gtgttgccgg
     1141 tgggtattct gaaggcgaac ctgtggagcg aggtggcggc ggacatcgac ggggttgtcg
     1201 cggcggcccg gcaaaggtcg ggcacaccgg cgctggtgaa ggtgattatc gaggcccctc
     1261 tcctcaccga ccgcgaaaag gcgatcgcct gcatcattgc cgagcagtgc ggcgcggact
     1321 tcgtaaagac ctgcacgggg ttttcgggcg ccgccacggt ggaggatgtg gcgcttatgc
     1381 ggcgcgtggt gggggatcgg ctgggcgtga aggcggcggg cggcatcaga acgcgggagc
     1441 aggcgctggc gttcgtccgc gccggtgcaa cgcggatcgg gacgagcaga ggtccggcgc
     1501 tggtgtaggc tttcagaacc agccacttcg acaagcttcc acgcagcggg tgcagctcgg
     1561 tgatcggttc cttgcccgtg cgcggcggct gagccctgtc ggtttttgca gatgatccgg
     1621 acgctcaggg aatatgctga cgggcccgac ttgggagtcg gtcgtccatt gcggcaaaaa
     1681 accgaactgc tgggagtttc gcgggcgcag atttggtttc cggaaaccgg tgaggtaaat
     1741 tcagcgttaa gggagaaaag ggtggagatt tcgtatgcga ataagagagt ggcgaagcac
     1801 ttcgctgaca gaaaggagct agaaagacgg tacgggaaac cttgcgcagt cgcgatcatg
     1861 aggcgaatca cagacctgca ggcggctccg aacctcgccg cgttgtcctt ttcggaagat
     1921 ccgagcgcca ggtgtcatga gctcaaacac gacttgaagg gatatctggc aatggacgtg
     1981 tcgaagagcc gacggttgct ttttgcgccg ggaaatgaac cgatcccaag gaagtcagac
     2041 ggcgggcttg actgggtccg ggtcacctgc gtcgtgattc atcgagtgga ggattatcac
     2101 aatggctaag gtggcgatga gtgcctttcc gccgaactgg gttgtcaccc ccggggagtt
     2161 ggtgcaggac catctcaatg gggccggcat cacgagaagc agtttcgttg aaaaaacggg
     2221 tttggcagcg gagacgttag atgcagtgat cgcggacgag atccgcatca cagaggatat
     2281 tgccgaggcg cttgccaccg ccctcggtct gcccgtttcc ttctgggtca atctgcaggc
     2341 acatttcgac agcgaggcaa agcgcctaaa aatcccgttg aagtacgttt ctgcggggca
     2401 atcacacaga aacacgggag cagctgcagg cacacgggtc cccgcgtagc acgggaatca
     2461 tggtgatggc cacaaatcct cggcttcgag aggagccggg ggtttttgtt cgctggcgac
     2521 cgggcaacgc cgccagttgg tggagaaacc ggctatgggc cctctcctca ggtgcgtatc
     2581 ctttttcgtg ctccccttcc tcattcccca gtctgcccac ggaggcgctt ccatgaccaa
     2641 cttgctttcc aacggcggat ttgaggatgc ggcggacggt atcccggtcg ggtggacgta
     2701 ttccgggccc gcggcgcggc aggacgagac gtggcctgaa ggtggggcac acgggggagt
     2761 gcgttgcgtc cgttttgcag cgcgaacgga ccggcaggcc tggacaagcg agcgtgtgcc
     2821 ggtgcagggg ggcatgccgt tccggctgga gtggtggaca ttgctcgcgg gcagcattcc
     2881 gtggcattgg acgacgcaca ccggcttcat cggggtgcgc gtgacgttct tcgacgcggc
     2941 gggtgaccgg tgcggcatca ccgagcggcg catccgctcc atcggaacag atggttgggt
     3001 tcggtgctgg ctggatgtca acccggcgcc ggaggcttcg tcgtgcacgg tgtccctcgt
     3061 gtgtgaggcc gaaatcgaga cggacggcac cgtcgcgttt gacgacgtgg cgctcgtgcc
     3121 ggccgggatg acccggacgc ccgccgggcg gggccatttg cgcggggtgg tgtggacggc
     3181 agaggatccg gcgccggggt tcgcgcgggt gttgatcacc gccgcggatg ggcgccacta
     3241 cgctccggag aacagctacc agttcctcga cgggtcgttc cacgccacgg gagagccgtt
     3301 tgagcttgcg ctccctgcgg gtcgtgcggc ggtgttggtc acgcgcgggt ttgagtattc
     3361 gatctggcgg gatacggtgg acatccgggc agggcagagg accgaggttg ctccccggct
     3421 ggcgcaccgg tggccgcttt ccgccgaggg atggtacagc ggggacgcgc acattcacct
     3481 gttcttccac cggcattcgc tccacccgca gatggttccg gcagacgtgt tcgccctcgc
     3541 gcgggcggaa gggctcggct ggctttcgtt caagggggag gagaaggagg cgcgggagta
     3601 cattgcgcgt tctgctccgc tgtgcgtacc gggattcacc ggagagatcg gcattgaggc
     3661 ggtgagcgat ttccacgggc acgtgtatac cctcaacgcg cggacgatac cggcggatgg
     3721 cttcccgatg aagctcgtgc cgtggccgat gaacctcgat ctgggcgccg cgctggattc
     3781 cgtcggcggc gcgctcgtgt atgcgcaccc gtcgggtgat ctggcggcgg atggagtgct
     3841 ggagggcatc ggcgacccgt cgcggcttct gctggcacgg gaatggccgg tggatctggc
     3901 gctgggacaa aac
//
LOCUS       DAOSOO010000266         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23544_length_3879_cov_4.126046, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000266 DAOSOO010000000
VERSION     DAOSOO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23544_length_3879_cov_4.126046"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(739..1119)
                     /locus_tag="PLT86_13530"
     CDS             complement(739..1119)
                     /locus_tag="PLT86_13530"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOT37678.1"
                     /translation="MDALPTYVTMCRAKLHNATVTETKLQYEGSITVDQNLLDAAGMY
                     LYEKVHVVNLNNGERFETYIIPGERDTGEVCLNGPAARLAEVGDTVIVIAYSFVPEAD
                     APAFRPTIVVLGPQNQLREILRPA"
     gene            complement(1196..2059)
                     /gene="panC"
                     /locus_tag="PLT86_13535"
     CDS             complement(1196..2059)
                     /gene="panC"
                     /locus_tag="PLT86_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT37679.1"
                     /translation="MRMITTVAEMRQWSDHERQSGTRIGFVPTMGALHAGHLSLVARA
                     RTKADVVAASIFVNPTQFGPREDFASYPRTLERDVDLLAQEGVAVVFAPSTEEMYPSG
                     CITTVRVEGLSAKLEGAIRPSHFAGVTLVVAKLFHIVQPHVAVFGRKDAQQAIILQRM
                     VRDLDFGVELDVAPTVREADGLAMSSRNVYLSIAERKQAVVLARALQAGFRAWTEGER
                     KSAVVRALMMNVLAEEPAARLDYLEIVSQRTLEPVAMVRSGTLVAGAIRVGKTRLIDN
                     WWVREDGEAEF"
     gene            complement(2050..2601)
                     /gene="folK"
                     /locus_tag="PLT86_13540"
     CDS             complement(2050..2601)
                     /gene="folK"
                     /locus_tag="PLT86_13540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT37680.1"
                     /translation="MNRSRCFLALGSNLGDRRENLRQALARVSCGGDLEIVAVSRLYE
                     TVPVGPPQGPYYNAVAEIVTGLAPEELLARCRSAENAGGRQRTVRWGPRTIDIDILDC
                     GGMVRETAELRLPHPEMARRAFVLVPLRDVAPDWVHPVEHRSVGELIEALGDTGGACV
                     VIEGSGWFVPPPGTRGEGASSCG"
     gene            complement(2598..3011)
                     /gene="folB"
                     /locus_tag="PLT86_13545"
     CDS             complement(2598..3011)
                     /gene="folB"
                     /locus_tag="PLT86_13545"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT37681.1"
                     /translation="MAKRVRTRSSVPADISHAPDVVRLRGMQFYGFHGATPQERKIGQ
                     RFEVDVEMAIDAEKVVDGDLSTTPDYRIVYGICNACVTERRYRLIEVMAREIAHEILA
                     TFPMVGVRVTVRKPCVPLGGVVTVGAEAVVQRGNL"
     gene            3167..>3879
                     /locus_tag="PLT86_13550"
     CDS             3167..>3879
                     /locus_tag="PLT86_13550"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT37682.1"
                     /translation="MERRYEHLRPADIIRLREAFPLAYVPLGTLEWHGPHNPVGLDGL
                     KAHALCLRAAEGGGGIVFPVVWYGEHRESHLMEINASVGKEIVTRMGLDPDDFKPGYT
                     QSGSILAQAQDYTALLWKICCQVKSLGFKGLIFLNGHYPLTHYGRFVGHLAARHLGLA
                     HWAGHEGQILEEAGEPGHGDHAGKWETSLLMALLPDEVNLQALSDAGEFIGCGRDALE
                     STAEEGEYWARRIVAALIA"
BASE COUNT          731 a         1219 c         1223 g          706 t
ORIGIN      
        1 agagcccgca ccgaggaaaa gagccgcggc aaaaagtgtg gacagccttc tcataccaga
       61 cctccaatgc gcccgaccgt ggaaatcaga caaatcgtca aattttgata agtttgcaca
      121 atgtaatgac gcaacccggc taaggctgat tttaacgtac gcgtgcgcgg gttttctgtc
      181 aatagcaaaa aaaaaacgcc tgtgaagttg atcacacgcc cccgcggcgc gggggtcgga
      241 tgccgtccgt gccgtgcgtt cgccactcgg aagccgtcat tcccgcgcag gcgggaatct
      301 aggggcagtc cgttgtccgt gcgcaggacg caatggttcg ggagggtcgt ctcgatacac
      361 cgcagcctga cggccgcgcc actcgacgac cgggaggagt gtcgcacgca ggaaaagagg
      421 aggaggctgg atacgcaaat gccgggctcc tcgatacgac gtccgtttcg cgtccgtcac
      481 tcggagaccg gctggccgga cgtacgacga ccggacatcg agtggcgaac cggcccggga
      541 acggaaacga agtgatgttc cccgtccggt tcgatgtatc gagatggccg gaccgctgcg
      601 gagaccgccg cagtccccct caacgaccgg cgcggggtgg cggcggcggt gatgtctgga
      661 ttcccgcctg cgcgggaatg acggcacagg ggcggaggga gcagacgtaa cggacttatt
      721 acaccctggt ttcatcgcct acgcggggcg cagtatttcc cttaattggt tctgtggccc
      781 cagcaccaca atcgtgggcc gaaacgcggg cgcatccgcc tccggtacga agctgtaggc
      841 gatgacgatg acggtgtcgc ccacctccgc aagccgcgcg gccgggccgt tcagacagac
      901 ttcgccggta tcccgctcgc ccggaatgat gtaggtttca aaccgctcgc cgttgttcag
      961 gttcaccacg tgcacttttt cgtacaggta cattccggcg gcatcgagga ggttctggtc
     1021 caccgtgatg ctgccttcgt actgcagctt cgtttcggtg acggtcgcgt tgtggagctt
     1081 cgcgcggcac atggtgacgt aggtcgggag ggcgtccatg gtactacgtt cctttcaaag
     1141 gggactgaat atgcgttccg tgtaacgaat ggcttaccag gcgatctggc gggcgttaga
     1201 actccgcctc gccgtcctcg cggacccacc agttgtcgat caggcgggtt tttcccacgc
     1261 ggatggcccc ggcgacaagc gttccgctgc gcaccatcgc aacgggctca agcgttctct
     1321 gcgacacgat ctcgaggtaa tccaaccgcg cggccggttc ttccgccagc acgttcatca
     1381 tcagcgcccg caccaccgcg cttttccgct cgccttcggt ccatgcgcgg aaaccggcct
     1441 gcagcgcgcg ggcaagcacg actgcctgtt ttcgttccgc aatcgagaga tacacgttgc
     1501 gcgagctcat cgcaagtccg tccgcctcgc gcacggtggg ggcaacgtcg agttcgacgc
     1561 cgaagtcgag gtcccgcacc atgcgctgaa gaatgatggc ctgttgcgcg tccttccgtc
     1621 cgaataccgc cacgtggggc tgaacgatgt gaaacagctt ggcgacgacc agggtaacgc
     1681 cggcgaaatg cgaaggccgg atggctccct cgagcttcgc cgaaaggccc tccaccctca
     1741 ccgtggtgat gcagcctgag gggtacatct cctcggtgga aggcgcaaac accaccgcaa
     1801 cgccctcctg cgcgaggagg tcaacgtctc gttcgagtgt ccgcgggtag gaagcaaaat
     1861 cctcccgggg cccgaactga gtagggttga cgaaaatgct ggccgcgacg acgtccgcct
     1921 tcgtgcgggc acgtgcgaca aggctgaggt gtccggcgtg cagcgccccc atggtcggca
     1981 cgaacccgat gcgcgtgcca ctctggcgct cgtggtcaga ccactgccgc atttcggcga
     2041 cagtcgtaat catccgcatg atgacgcacc ttccccgcgc gttcccgggg gtggcacgaa
     2101 ccagccactc ccctcgatta ccacacacgc tccgccggta tcacccagtg cctcgataag
     2161 ttccccgacg ctccgatgtt cgaccggatg cacccagtcc ggcgcgacat cgcggagcgg
     2221 aacaagcaca aacgcccggc gtgccatttc cgggtgcggc agacgcagtt ccgcggtttc
     2281 gcgcaccatc ccgccgcagt cgagaatgtc aatgtcaatg gtgcgcggtc cccagcgcac
     2341 cgtccgctgg cggccgccgg cgttttccgc gctccggcac cgcgcgagaa gttcctccgg
     2401 cgcgagcccg gtcacgattt ctgccaccgc gttgtaatag ggcccctggg gcggtcccac
     2461 cggaaccgtt tcgtagaggc gggagaccgc aacgatctcc agatcgccgc cacacgatac
     2521 cctcgcaagg gcctggcgaa gattctccct gcggtcaccc aggttcgatc cgagggccag
     2581 aaagcaccgt gagcggttca aaggttgccc cgttgcacca cggcttcggc gcccaccgtc
     2641 accacgcctc caaggggcac gcacggtttc ctcaccgtta cccgcacgcc gaccatcgga
     2701 aaggttgcca gaatctcgtg cgcaatttcc cgcgccatca cctcgatcag ccggtaccgc
     2761 cgttcggtga cgcaggcgtt gcagatgccg tagacgatgc ggtaatccgg ggtggtggag
     2821 aggtcgccgt ccacaacctt ttcggcgtca atggccattt ccacgtccac ttcgaatcgc
     2881 tggccgattt tgcgttcctg aggggtcgcg ccgtgaaatc cgtagaactg catgccccgc
     2941 aggcgcacca catccggcgc gtgggagatg tccgcgggaa ctgaggagcg agttcgaact
     3001 cgtttcgcca ctgtggcccc gtttcttcgc gtcaatcgca actcgtagaa ttatacacta
     3061 cgcggcgaga cgaaataggt caagcggcgg catgcggccc ggtgtgtcct gcgttcgcgt
     3121 gcaatgccgg gacgccgact caggcccgcg ccagagggga gacaaaatgg agcgtcggta
     3181 cgaacacctc cgccccgcgg atatcatacg gctgcgggag gcttttccgc tggcatacgt
     3241 gcctctcgga acgctcgagt ggcacggtcc gcacaatccg gtggggctgg acggcctgaa
     3301 agcgcacgcg ttgtgcctcc gcgcggccga aggaggcgga ggaatcgtgt ttcctgtggt
     3361 gtggtacggc gaacaccggg agtcgcacct gatggagatc aatgcgagcg tgggaaagga
     3421 aatcgttacg aggatgggtt tggatcccga tgattttaag cctggctata cgcagtcggg
     3481 ttctatcctt gcccaggcac aggattatac cgcgctgcta tggaagattt gctgccaggt
     3541 gaaatccctc gggttcaaag ggctgatttt tctgaacgga cattatccac tgacccatta
     3601 cgggcggttc gtcggccatc tcgccgcgcg ccatctcggc cttgcacact gggcgggaca
     3661 cgaaggccag attctggaag aggcaggcga accggggcac ggagaccacg caggcaagtg
     3721 ggagacctcc ctgttgatgg cgctcctgcc ggacgaggtg aaccttcagg cgcttagcga
     3781 cgcgggggag ttcatcggct gtgggcgcga cgcgctggag agcaccgctg aagaggggga
     3841 atactgggcg cgcaggattg ttgccgcact tatcgcgaa
//
LOCUS       DAOSOO010000267         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23645_length_3866_cov_3.776699, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000267 DAOSOO010000000
VERSION     DAOSOO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23645_length_3866_cov_3.776699"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLT86_13555"
     CDS             <1..211
                     /locus_tag="PLT86_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37683.1"
                     /translation="PEPSSVRLVVFDLNGRVVRTLVDQPMQAGMHSVVWDGQDALGRD
                     AASGVYLYTVTTSRGASTKRMVLVR"
     gene            328..2331
                     /locus_tag="PLT86_13560"
     CDS             328..2331
                     /locus_tag="PLT86_13560"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT37684.1"
                     /translation="MARTQPPDVTKADMQQCLDWTGGFFASPAALPVSYTYGGKGYRG
                     IPAEFAPKIRTRRIDACIIATSIEGRDRATGLLVRAEIVRYLDFPVVEWTVYFTNEGE
                     KDTPILSDIQGLDADFAGTNPVLWHCNGDYYSIDGYTPFETPLNEGASVTIAPNGGRS
                     CNGAFPYFRVMTADAVLTFAVGWPAQWSATFSGTATGAHITAGQQLTRMKLLPGETMR
                     TPRISLLAVTGDMQRSINVWRRWYQAHVLPRSDGQPLPQKLVAHAVPFGDEFTEATEE
                     NQIAYMEKFAQKGYKFSTWWIDAGWYPCRMPNGKGDWPTTGTWIPDPPRFPNGFKPVS
                     DAAHKHGADLLIWFEPERVRKGTQIEKEHPEWLLGLKSHPDNNDKLLNLGNPACRQWL
                     TDHVSTLIQEGGIDIYRQDHNFDPLEFWRQNDAEDRQGANENLHVQGYLQYWDDLLAR
                     NPGLWIDSCASGGRRNDLETMRRSVPLHYTDFGYGIHYVKLSFHHTLYQWIPYIKECT
                     LSWDKDYTTFQRFEEQIDRFAFHCGIAAMTTPAVDINRDDYDYAQAAKLTELAFKTGE
                     YFIHGDYYPLTPFARAEDKWVVRQFHRAADGTGFVQGIRLQKCPEESITVFPQGLDDT
                     LRYVFEDAETGEKREVSGAALRVTGFTLKQPVRSGAVWLYRPV"
     gene            2467..2907
                     /locus_tag="PLT86_13565"
     CDS             2467..2907
                     /locus_tag="PLT86_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37685.1"
                     /translation="MRFRTLIATVAPLFLAVALAISGQGCGAASPLDSGISTEEVIVD
                     FFHPVTDSFAQDFAARNGLIIRYKSSFVPSYKFSIDRSVTPRHVTAAMVAQRLLSAYP
                     DLIRHAEPESYGQGYTVRAARDMRTRGGNERVCEISRPRNVSLC"
     gene            3214..>3866
                     /locus_tag="PLT86_13570"
     CDS             3214..>3866
                     /locus_tag="PLT86_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37686.1"
                     /translation="MLDVVSYAVATGRRVICLTDHWWKFTRVPNKPQPHYAPTMDGYR
                     QFAADAAEVRAMFPDGCLLFGPEIGFGSFESEEIGQAFAVPGVDMFLGEPGAAPPGTR
                     YGDYLIAGLEDIARCRDRFRRPGTLVHPIRAAIKRVCGDAGPGPLHPKHPQFPPLGSV
                     ADPLTHVEELLDVNIGPLADTCVRLDVPVEINMLDFKRILAHNHQSFAERYLFFYRTL
                     "
BASE COUNT          734 a         1243 c         1206 g          683 t
ORIGIN      
        1 acccgaaccg tcatccgtgc gcctggtcgt gttcgatctg aacgggcgtg tcgtccgcac
       61 gctcgttgac cagccgatgc aggcggggat gcattccgtt gtgtgggacg ggcaagatgc
      121 tctgggaaga gacgccgcca gcggtgtgta tctgtacaca gtcacgacat cgcggggcgc
      181 ctcaacgaag cgcatggttc tggtgaggta ggaattcacg ccgtccgcaa tgccccaata
      241 gcccccaaga gccttctgga ttatccttac agaaggctct tttcgttgta cagtctcccg
      301 ctttccctgc tttgagagag gtgtcagatg gccagaacgc aacctccaga cgtcacaaaa
      361 gccgatatgc agcagtgcct ggactggacc ggcggctttt tcgccagccc ggcggcgctg
      421 ccggtttcct acacctatgg cggcaagggg taccgtggta tcccggcgga attcgcgcca
      481 aaaatccgca cccgccggat agacgcctgc atcatcgcga cctccatcga ggggcgcgac
      541 cgcgcaaccg gcctcttggt gcgggcggaa atagtgcgct acctcgactt cccggtcgtg
      601 gagtggacgg tctacttcac gaacgaaggc gagaaggaca cgcccatcct gagcgacata
      661 caggggctcg acgccgactt cgccggcacg aacccggtgc tctggcactg caacggcgac
      721 tactacagca ttgacgggta taccccgttt gaaacgccgc tgaacgaggg cgccagcgtt
      781 actattgccc cgaacggcgg gcgctcgtgc aacggcgcgt tcccctattt ccgcgtgatg
      841 acggcagatg ctgtacttac tttcgcggta ggctggcccg cgcagtggag cgcgacgttc
      901 tcgggcaccg ccacgggcgc gcacatcacg gcggggcagc agctcacccg catgaagctc
      961 ctccccggcg aaaccatgcg cacgccgcgc atttccctgc tcgccgtaac cggcgatatg
     1021 cagcgctcga tcaacgtctg gcggcgctgg taccaggcgc acgtgctccc gcgctcggat
     1081 gggcagccgc tcccgcagaa gctggtggct cacgcggtgc cgttcggcga tgagttcacc
     1141 gaggcgacgg aagagaacca gatcgcctac atggagaagt tcgcccagaa gggatacaag
     1201 ttcagcacct ggtggattga tgcgggttgg tacccctgcc gcatgccgaa cggcaaaggc
     1261 gattggccca ccaccggcac atggattccc gatccgccgc gcttcccgaa cgggttcaaa
     1321 ccggtctccg acgccgcgca caagcacggc gcggacctgc tcatctggtt cgagccggag
     1381 cgcgtccgga agggcacgca gatcgaaaag gagcaccctg aatggctcct cgggctcaag
     1441 agccacccgg acaacaacga caaactgctc aacctcggca atcccgcgtg ccgccagtgg
     1501 ctgacggacc acgtcagcac gctcattcag gagggcggga tagacatcta ccggcaggac
     1561 cacaacttcg atccgctgga gttctggcgg caaaacgacg cggaggaccg gcagggcgcc
     1621 aacgagaacc tccacgtgca gggctacctg cagtactggg acgacctgct cgcccgcaac
     1681 cccggcctgt ggattgattc ctgcgcctca ggcggccgcc ggaacgacct cgaaaccatg
     1741 cgccgctccg tgcccctgca ctacaccgac ttcggctacg gcatccacta cgtcaagctc
     1801 tccttccacc acacgctgta tcagtggatc ccctacatca aggaatgcac gctctcgtgg
     1861 gataaggact acaccacgtt ccagcggttc gaggagcaga ttgaccggtt cgcgttccac
     1921 tgcggcatcg ccgccatgac gacgccggca gtggatatca accgggacga ctacgactac
     1981 gcgcaggcgg cgaagctgac ggagcttgcg ttcaaaacgg gcgagtactt catccacggc
     2041 gactactacc cgctcacccc gtttgcgcgc gcagaagaca agtgggtcgt gcgccagttc
     2101 catcgcgcgg cggacggcac cgggttcgtg cagggcatcc ggttgcagaa gtgcccggag
     2161 gaaagcatca ctgtcttccc gcaggggttg gacgacaccc tccgttacgt gttcgaggat
     2221 gcggaaaccg gggaaaaacg cgaggtctcc ggcgcggcgc tcagagtcac gggattcacg
     2281 ttgaagcaac ccgtccgcag cggcgcggtg tggctttacc ggcccgtgta aggccggtca
     2341 ggccggtgtg agcccggcga ggtcgtcgag tggcgagctg cccccgggca gcctgctgcc
     2401 cccgggcagc ttgctgcccg cagggcggca agtgtatcga gacgcaccga tgaagggagg
     2461 tgtaggatga gatttcgtac cctgatcgcc acggtggcac cattgttcct cgccgtcgcg
     2521 ttggcaatct cggggcaggg ctgtggcgcg gcttcgccct tggactctgg catttctacg
     2581 gaagaggtta tagttgattt cttccacccg gttacggaca gctttgctca ggattttgcg
     2641 gcaaggaacg gcctgatcat ccgctataag agttcattcg tcccctcgta caaattcagt
     2701 attgatcgtt cagttacgcc caggcatgtt acggcggcga tggtagcgca gaggttgctt
     2761 tcggcctacc ccgatctcat ccgccatgct gaaccggaga gctacgggca agggtacacc
     2821 gtgcgcgccg cgcgggacat gcgtactcgc ggagggaacg aacgcgtttg cgagatctcc
     2881 cggccccgga acgtgagctt gtgctgagcg tgggcgatac cgtacggagt accagcactg
     2941 acacgccccc gggtcaccgt catccccatg aagacacggc aatggggcgg cgttggtcag
     3001 cgggcaaccg caatccggtc atcgagtggc gaacgcagcg cgcgtgtcgc gcacctgcgg
     3061 ttcgctgtat cgagatgacc gacagccaaa agaccgcggc tttccacaaa accaaacagc
     3121 gaggtggatt tgttccccac atggaacgat cttgaactgc acgccgttga cctccacttc
     3181 cacgtcggta cggcgcggcc cgaggaaaac agcatgctcg acgtggtcag ctacgccgtg
     3241 gcaacagggc gccgggtgat ctgcctgact gaccactggt ggaagttcac ccgtgttccg
     3301 aacaagccgc agcctcacta cgcgccaacg atggatgggt accggcagtt tgccgccgat
     3361 gccgcggaag tccgtgcgat gtttcccgac gggtgcctgc tcttcgggcc ggagatcggg
     3421 tttggcagct tcgagtccga ggagatcgga caggcgttcg cggtacccgg cgtggatatg
     3481 ttccttggcg agccgggtgc cgccccgccc ggcacgcgat acggtgacta cctcatcgcg
     3541 ggactcgaag acatcgcgcg gtgccgcgac cggttcaggc gaccgggcac gctggtacac
     3601 ccgattcgcg ccgctatcaa gcgcgtgtgc ggggacgccg gccccgggcc gttgcacccg
     3661 aagcacccgc agttcccgcc gctgggttcc gttgccgacc cgctgactca cgtcgaggag
     3721 ctgctcgacg tcaacatcgg ccctcttgcg gatacctgcg tccgtcttga cgtgccggtg
     3781 gagatcaaca tgctggactt caagcggata ctggcgcaca accaccagtc ctttgcggag
     3841 cgatacctgt tcttctatcg cacgct
//
LOCUS       DAOSOO010000268         3838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_23845_length_3838_cov_5.060798, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000268 DAOSOO010000000
VERSION     DAOSOO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23845_length_3838_cov_5.060798"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLT86_13575"
     CDS             <1..965
                     /locus_tag="PLT86_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37687.1"
                     /translation="PVYDVGVLIPKDQWKDELGTWAVCLLKHHHAFCQVPLHVRDFSI
                     FRVVVVADGYEMTPDLAAALEEYASQGGNVVVECNGAGVCTEYGDMFLLSKLLGVSAN
                     GRHGFDVSYIGGIAGELADGLWDGPIRAGADGFRVVPVTARVLARYVYPVARYSSQRW
                     IWREPNPPRAEISGEAAITVNHFGKGKAVFVACPAGLDEAPQRRGVMKLCSNIVSYLC
                     PEELWRTNAPSGVEIVLSKKEGKYLLHVLNHYVEQSSRFDTRDDSIPSLAGFDILVNT
                     KRVGSLGKVTCGSTGASVPSTHINDYMRIHVDTLAIQEMYLLEP"
     gene            985..2367
                     /locus_tag="PLT86_13580"
     CDS             985..2367
                     /locus_tag="PLT86_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37688.1"
                     /translation="MQYNVGTRRELFVDGTLVQSFQGTRLVLQKPERRETVLELDAPW
                     ENNVAFPYRVLPFKGGWRMYYRASILDLSREEDTCVTAVAESDDGVSFRRPSLGVCEF
                     AGSRQNNILQIGGFPNTPPPFFDTRPGCPPEERFKGIAARQSQAFAMVSGDGVRWRLL
                     HNTPMHLRGQFDTVNTAFWDELAGCYRSYTRSWHDPETGAAFPTWDFGGVAAVRAIQH
                     ARSEDFLNWTEPEQLQYADGDRTVHLYTNAIVPCPEAEHILLGFPSRFVPDRTPDESH
                     GLPGVNDALFMTSRDGVHWNRWLDAWVSPGLDPLNWTERNNYPVWGIAKTSPTEWSMY
                     ISEHYRHEGVPTRVRRLSIRPWGFVAVHADYSGGELVTKPLVFEGTSLSLNARTSAAG
                     AIRVEIQDETGHAIPAFSLAEMRPWYGDSLDARMAWGENTSLGSLRGRPIRLRFWMKD
                     ADVFSFRFPS"
     gene            2387..3550
                     /locus_tag="PLT86_13585"
     CDS             2387..3550
                     /locus_tag="PLT86_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37689.1"
                     /translation="MTDLERFVAICRGETPDYVPIFGFPGAPGMSDGAMRKTHERLVA
                     TGMPSSVGGASSLIYGNPERESWRRYWGTTGPLTLDFGLAGGAQGFRTKRRFENGFEI
                     IESESGEITRQVVENDVTYSMPEFVRYPVRDRESWEFYKERTRSRRVMSREEMEANCR
                     RYETRDRPLCVGAGSTYGHIRGLMGTEGASVTLIEDPELAHDIIETGLKYHREFVFPL
                     IERLRPEVVQMGEDLCFNHGMLLSPRHFREFCGEYYREVCDCAFANGAEIVAIDTDGN
                     AMEFTGVASEYGVNAIFPYEVKAGNDLFELRQNHPEFVFFGWLEKEVVNEGNEALIEP
                     EILSKVPQLLARGGYFPNGDHGIQPPVTFRNLCRFMTLLHEACGNPKGTFPRV"
     gene            3596..>3838
                     /locus_tag="PLT86_13590"
     CDS             3596..>3838
                     /locus_tag="PLT86_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOT37690.1"
                     /translation="MDTSRLFDVASFGARSHAPETSTHAFQKAIDACAESGGGTVFVP
                     PGEYVIGRVLLRSNVTFHLAAGALVKPSREKRDYPPI"
BASE COUNT          815 a         1030 c         1169 g          824 t
ORIGIN      
        1 aaccggtgta cgacgtcggg gtgcttattc cgaaggacca gtggaaggac gaactgggta
       61 cgtgggcagt gtgtctgttg aagcatcacc atgcgttctg ccaggttcca cttcatgtgc
      121 gggatttttc catattccgt gttgttgtgg tggcggatgg ttacgaaatg actccggatc
      181 tggcggcggc tcttgaagag tatgcgtcgc aaggtggaaa cgtggtggtg gagtgcaacg
      241 gcgcgggggt gtgtactgag tacggggaca tgtttcttct ctcaaagctg ctgggtgttt
      301 cggccaatgg ccgccatggt ttcgacgttt cgtacatcgg aggaatcgcc ggagaactcg
      361 cggatgggct gtgggatggc cccattcgcg ccggcgcaga cggctttcga gtggttcccg
      421 taacggctcg cgttcttgcc cgttatgtct atccggtcgc ccgttattcg tcacaacggt
      481 ggatatggag agagccgaac ccgccgcgcg cagagatttc gggcgaagcc gccatcaccg
      541 tcaatcattt tggcaagggg aaagccgtgt ttgtggcgtg cccagcaggt cttgacgaag
      601 cgccgcaacg ccggggcgtg atgaagcttt gttcgaacat tgtctcgtac ctctgcccgg
      661 aggaattgtg gcgcacgaac gctccttcag gtgtggaaat agtgctgagc aagaaggaag
      721 ggaaatacct cctccacgta ctgaaccact atgttgagca aagcagtcgg ttcgacacgc
      781 gcgatgattc gattccctca ctcgcgggtt tcgacattct cgtgaatacg aaacgggtcg
      841 ggagtttggg gaaagtgaca tgtggttcca ctggcgcatc agttccatca acacacatta
      901 atgattatat gcgcatacac gtagatacac tggcaattca ggaaatgtat cttttggaac
      961 cgtgattcca gaggagacag caccatgcaa tacaacgtgg ggacaaggcg cgaactcttc
     1021 gttgacggaa ctctcgtgca gtcgtttcag gggacccgac ttgttcttca gaagccggaa
     1081 cgtcgcgaga cggtactgga gttggacgca ccgtgggaaa acaatgttgc cttcccttac
     1141 agggttcttc ccttcaaagg cgggtggcgc atgtactaca gggcttccat cctcgatctt
     1201 tcccgggagg aagacacctg tgttactgcc gttgccgaaa gcgatgacgg cgtgagtttc
     1261 cgccgacctt ctctcggggt gtgcgagttt gcgggttcca ggcagaacaa tattctccag
     1321 atcggaggtt ttccaaacac gccgccgccg tttttcgata cgagaccggg ctgtccaccg
     1381 gaggagcgtt tcaaaggaat tgctgcgcgg caatcccagg cctttgcgat ggtctccggc
     1441 gacggcgtgc ggtggcgcct gcttcacaac acaccgatgc acctccgtgg gcaattcgac
     1501 accgtaaata cggccttctg ggacgaactc gccgggtgct accgatcata tacgcgcagc
     1561 tggcacgacc ctgaaaccgg cgcagccttc ccgacgtggg attttggcgg cgtcgccgcg
     1621 gtccgggcga tccagcacgc gcgttccgaa gacttcttga actggacgga gccggaacaa
     1681 ctgcagtacg cggacggcga ccggacagtc catctgtaca cgaacgcgat cgttccatgc
     1741 ccggaagcgg aacatatcct ccttggtttt ccgagtcggt tcgttccaga tcgaacaccg
     1801 gacgaatccc acgggttgcc cggggtcaat gatgcattat tcatgacgag cagggacggg
     1861 gtgcactgga atcgttggct ggatgcgtgg gtgtcgccag ggctggaccc gttgaactgg
     1921 acggaacgaa acaactaccc cgtctgggga attgcgaaga cctcgcccac cgaatggtcc
     1981 atgtatatct ccgagcatta tcgccacgag ggcgtgccga cacgcgttcg caggttgagc
     2041 atccggccat ggggatttgt tgccgtacac gcggactatt ccggcggcga gttggtcaca
     2101 aagccgctcg tctttgaggg aacttcgctg agtctgaacg cacgcacctc cgcagcaggc
     2161 gccatccgcg tggagattca ggatgaaacg ggccatgcaa taccggcgtt ctcccttgcc
     2221 gagatgcggc cgtggtacgg cgattcgctc gatgcgcgga tggcctgggg cgaaaacacc
     2281 agcctcgggt cccttcgcgg gcggccaata cggcttcgct tctggatgaa ggatgctgac
     2341 gtgttttcgt ttcgttttcc ttcctgaaag gaactcgaaa aagtaaatga ctgacctgga
     2401 acgatttgtc gcgatctgcc ggggtgaaac acccgattac gtacccattt tcggttttcc
     2461 gggtgcaccg ggaatgagcg acggcgccat gcgcaaaacg cacgaacgac tggtcgccac
     2521 aggaatgcct tcgtccgtcg gaggggcgtc gagcctcatt tacggtaacc ctgaacggga
     2581 aagctggagg agatactggg gcaccacagg gccacttacc ctggacttcg gcctggctgg
     2641 cggagcgcaa ggattccgca cgaaaaggcg gttcgagaac ggttttgaga tcatcgagtc
     2701 cgaaagcggg gaaatcacgc ggcaggtggt ggaaaatgac gtcacctaca gcatgcctga
     2761 gtttgtcagg taccctgtcc gagaccgcga atcctgggag ttttacaaag aacgcacgcg
     2821 ctcccggcgg gtaatgtcgc gcgaggaaat ggaggcgaac tgccgcaggt atgaaacgcg
     2881 cgacaggccg ctgtgtgtcg gagcggggag cacgtacggg catatacgcg gccttatggg
     2941 aaccgaaggc gccagtgtga ccttgatcga ggatcccgaa cttgcgcacg acatcattga
     3001 aacggggctg aaataccaca gggaatttgt gttcccgttg atagaaaggc tcagaccgga
     3061 agttgtgcag atgggggagg atctttgctt caatcacggc atgctgcttt ctccccgcca
     3121 ttttcgggaa ttctgcggtg aatactaccg tgaggtctgc gattgtgcct tcgcgaatgg
     3181 cgcggagatt gtggccattg ataccgatgg gaacgccatg gaatttaccg gcgtcgcctc
     3241 cgaatacggc gtcaacgcga tattcccgta cgaagtcaag gctggaaacg acctgtttga
     3301 acttcggcag aatcaccccg aattcgtctt cttcggctgg ttggaaaagg aagtagtcaa
     3361 tgagggaaat gaagccctca tcgaaccgga aatactgtca aaagttcctc agttgcttgc
     3421 acgcggaggc tacttcccta acggcgatca cggtattcaa cctcctgtca cgttccgcaa
     3481 tttgtgccga ttcatgacct tgctgcacga agcctgcggg aaccccaagg ggacgttccc
     3541 ccgagtctga agccagccac cagtggtgcc aagagtccac aggagtacgc gcgcgatgga
     3601 cacatctcgt cttttcgatg ttgcatcgtt cggggcgcgc tcacatgcgc cggaaacaag
     3661 cacacacgca tttcagaagg ccatcgacgc gtgtgccgaa tccggcggag ggaccgtttt
     3721 cgttcccccc ggtgaatacg taatcggcag ggttcttctt cgcagtaacg taacctttca
     3781 ccttgcagcg ggagccttgg tcaagccctc gcgggagaaa agggattatc cgcccatt
//
LOCUS       DAOSOO010000269         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24022_length_3817_cov_4.946837, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000269 DAOSOO010000000
VERSION     DAOSOO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24022_length_3817_cov_4.946837"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PLT86_13595"
     CDS             <1..1378
                     /locus_tag="PLT86_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT37691.1"
                     /translation="VEDVLNIHDREQPDGVIVQFGGQTPLKLAVPLEKSGVKIIGTSP
                     AMIDVAEDRKLFGALINRLGIRQPENGTARSEEEAEAVASQIGYPVLVRPSYVLGGRG
                     MAIVYDRDSLVHYMRAAVLASPEHPVLIDRFLDDAIEIDVDAVSDGTLTVVGGIMQQI
                     ELAGVHSGDSACVLPPYRLTPDMIEKLMRHTKDLARELHVVGLMNVQYSIKDDEVYVL
                     EVNPRASRTVPFVSKAIGVPLAKIAARCMVGKSLAEQGFTSEIVPPYVSVKEAVLPFL
                     KFPGVDILLSPEMRSTGEVMGISESLGESYAKSQLAANLRLPDRGTVFISVSGDEQKR
                     ALVEPARRLAELGFDIIATEGTLAMLRRNGVPAMFVPKLQEGRPHVVDMMKSSKIALV
                     INVPHGKAASQDEIAIRRETIRRGLPYMTTVAAAVAAVEGIAALRAEEMSVRSLQEHH
                     ARIHRPTQ"
     gene            1441..>1682
                     /locus_tag="PLT86_13600"
     CDS             1441..>1682
                     /locus_tag="PLT86_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37692.1"
                     /translation="MSGSWERLGVHLMNQPFFLPAVDEAAEHETAGLSGGLQPRCVSC
                     KACREPVWVVSDSRGVSFALSRASYIGGEAAPVAWAP"
     assembly_gap    1683..1782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1974..2049
                     /locus_tag="PLT86_13605"
     tRNA            1974..2049
                     /locus_tag="PLT86_13605"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2007..2009,aa:Cys,seq:gca)
     gene            2150..3229
                     /locus_tag="PLT86_13610"
     CDS             2150..3229
                     /locus_tag="PLT86_13610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT37693.1"
                     /translation="METLPDTVDGPRGGTWYEFGEILDFLNFVFRSSVGRKPSIGGDY
                     PHLYNERNVHNFRHIRVNGRVISNVSIYPARVQWGDAVLKIGGIGGVATDPDYRKHGY
                     AGTILAHCLEYMDRELDCDLSILWTGIPDYYRRWGWEHAGQNWTFFIDRTTITYLPVA
                     PTGEVLTDTLDPRVIQAVESLHAARRAGVVRDRELTEVLLNIRTRVRCAVLMDGETPV
                     AYIVYSPGEQIDVKDCGGDGSAVLGLLRVVFAQCGARTAQIHTPAQDQSVAPLFIERG
                     FAVRANYSGMLAVLKPQRILEAYGIRDVTVRPEGDGWQIQTSTASVFLRKPDLVKLLF
                     GPERPPEIGHSKLPLPFYYGQLDHM"
     gene            3258..>3817
                     /locus_tag="PLT86_13615"
     CDS             3258..>3817
                     /locus_tag="PLT86_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT37694.1"
                     /translation="MRSKYRAECSPRGRWVVAHRGFSGRYPENTMAAFRAAVALGVDA
                     IEIDVHLSRDGHVVIMHDAKLDRTTDGKGPIAEHTLAEIKRVDAGSKKGAQFAGERVP
                     TFAEMLAEISLPVMCEIKDPGEDIVRETARVVKAAGAEDRVIIACFDDATLRWAEHYL
                     PACERLALGQSTAERIRTTHINAPSF"
BASE COUNT          719 a         1116 c         1172 g          710 t
ORIGIN      
        1 cgttgaggac gtgctgaaca tccatgacag ggaacaacct gacggcgtca tcgtccagtt
       61 cggcgggcag accccgctca aactcgccgt tcctctggag aagtcggggg taaagatcat
      121 cgggacgtcg ccggcgatga tagacgtcgc ggaggaccgc aagctcttcg gcgcgcttat
      181 caaccggctc ggaatccgcc aaccggagaa cggaaccgca cgcagcgagg aggaggcgga
      241 agccgtggca agccagatcg gctacccggt gctggttcgc ccttcctatg ttctgggtgg
      301 ccgcgggatg gccatcgtgt acgaccggga ttcgctggtg cattatatga gagccgcggt
      361 gctggcttct cctgaacacc ccgttctgat tgaccgattt ctcgacgatg ccatcgaaat
      421 agatgtagac gccgtttcgg acggtacgct gacggtagtt ggcggaatca tgcagcagat
      481 cgaactggcg ggagtccatt ccggcgattc cgcgtgcgtg ctgcccccgt accgcctcac
      541 cccggacatg atcgaaaagt tgatgcggca tacgaaggac cttgcccgcg agctccacgt
      601 agtcggtctg atgaatgtgc agtattccat caaggacgac gaggtgtacg ttctcgaggt
      661 gaacccccgc gcgtcgcgta cggtgccgtt tgtcagcaag gccatcggtg ttccgctggc
      721 gaagattgcc gctcgctgca tggtgggcaa aagccttgcc gaacagggtt tcacgtcgga
      781 aattgtaccg ccgtacgtct ccgtcaagga agccgtgctg ccatttctca agtttcccgg
      841 cgtggatatc ctgctcagcc cggagatgcg ttcgaccggc gaagtgatgg gtatttcaga
      901 atcccttgga gagagctacg cgaaatccca actcgcggca aatcttcgcc tgccggacag
      961 gggaactgtt ttcatcagcg tgagcggaga cgagcagaag cgggctctcg tggagcctgc
     1021 gcggcggctt gccgaactcg gtttcgacat catcgccacc gaaggcacgc tggcaatgct
     1081 ccgtcggaac ggcgttcccg ccatgtttgt tccgaagttg caggaaggcc gtccccacgt
     1141 ggtcgacatg atgaaatcgt caaagatcgc ccttgtgata aatgtgcctc acgggaaagc
     1201 cgcgtctcag gatgaaatcg ccatccgacg cgagacgatc cggcgcggcc ttccctacat
     1261 gacaacggtt gccgccgcgg tcgccgcggt ggaggggatc gcagccctgc gcgcggagga
     1321 aatgagcgtg cgttccctgc aggagcatca cgcccgcata catcgcccga cgcaatgagt
     1381 gcacgacaac gaaccagagt tctcggatct ccgcctcgct ccgggaagcc gctcgtcggc
     1441 atgagcggct catgggaacg gctcggcgtg catctgatga accagccgtt cttcctcccc
     1501 gcagtggatg aggcggccga acacgaaacg gcaggcctct ccggtggcct gcaaccccga
     1561 tgcgtatcct gcaaagcatg ccgggaaccg gtatgggtag tttctgattc ccgcggcgtc
     1621 tcgttcgcgc tctcccgcgc aagttacatc ggtggtgaag cagcgcccgt cgcctgggcg
     1681 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagacggcg gaaggaattg
     1801 ctgcgtgacc gagagcgctg cgagtttgtc atcgcggaat gggagaagcg gtatgaccac
     1861 ccgaatggct cgcggtgaag gacttctccc ggtggttggt cgtcaccacg gcagcgccgg
     1921 ggcagccgca ggtcttgata cgcttttccg tcgcgcgtac attgtaagaa tccggcggcg
     1981 tagccaaggg gctaaggcgg aggtctgcaa aacctctatt cggcggttcg agtccgcccg
     2041 ccgcctccac gttttccgaa gggacgggcg cgatcgcagg gcctgtccct ttttctttgc
     2101 gcttccggca tcgcggcacc tgaagccatt ctgagaggag aattgcccga tggagacgct
     2161 ccccgacacc gttgatggcc cacgcggcgg aacgtggtac gaattcggcg agattctcga
     2221 cttcctcaat ttcgtgttcc gctccagtgt cggccgcaag ccaagcatcg gcggggatta
     2281 cccgcacctg tacaacgagc ggaacgtgca caatttccgg cacatccgcg tgaacgggcg
     2341 cgtcatcagc aacgtgagca tctaccccgc ccgggttcaa tggggtgatg ccgtgctgaa
     2401 gatcggcgga atcggtggcg tggctaccga cccggattac cgcaagcacg ggtacgcggg
     2461 aaccattctg gcccattgcc tcgaatacat ggaccgcgag ctggattgcg acctgtccat
     2521 tctctggacc ggcatccctg actattaccg acgctgggga tgggagcatg cggggcagaa
     2581 ctggacgttc ttcatcgatc gcaccaccat cacctatctc ccggtcgccc cgaccggcga
     2641 agtgcttacc gatacactcg atccccgcgt cattcaggca gtggagtcgc ttcacgccgc
     2701 ccgcagggcg ggagtcgtgc gcgaccgcga gctcaccgag gttttgctca acatccgcac
     2761 gcgcgtccgg tgtgccgttc tcatggacgg ggaaacaccg gtggcgtaca tcgtgtactc
     2821 acccggtgaa cagatcgacg tgaaggattg tggtggggat ggttcggctg ttctggggct
     2881 tctgcgcgtc gtgttcgccc agtgtggcgc ccggacggcg cagattcaca ctccggcaca
     2941 ggaccaatcc gtcgcgccac tttttatcga gcgcgggttc gccgtccgcg ccaattactc
     3001 cggcatgctg gctgtcctga agccgcagcg catcctggag gcgtacggaa tccgggatgt
     3061 gactgtacgg cctgaagggg acgggtggca gatccagacc tcgacggcga gtgtgttttt
     3121 gcgcaaaccc gatctggtga agctcctgtt cggacctgaa cggccgccgg aaatcggcca
     3181 ttccaaactg ccgttgccgt tctactacgg ccaactggac cacatgtaac gttcacaagt
     3241 aatcgggagt gcgtgcgatg agatcgaaat accgcgctga gtgctcgcca cgcgggcgat
     3301 gggtggttgc acaccggggt ttctcgggca ggtacccgga aaacaccatg gcggcattca
     3361 gagcggcggt cgcgctggga gtggacgcta tcgaaattga cgtccatctc tctcgcgatg
     3421 gtcacgtggt catcatgcat gacgcgaaac tggaccgcac gacggacggg aagggcccga
     3481 ttgccgaaca cacgttggcg gagatcaaac gggttgatgc cggctccaaa aaaggcgcac
     3541 agttcgcagg cgaaagagta cccacgttcg cggagatgct cgccgagatc tcgcttcccg
     3601 taatgtgcga aatcaaggat ccgggtgagg atatcgtccg ggaaacggca cgggtggtga
     3661 aggctgcagg ggccgaagac cgcgtcatca tcgcatgctt cgacgacgcg actctccggt
     3721 gggctgagca ttacctgcct gcctgcgaac gccttgctct ggggcagtcg acggcggaac
     3781 gcatccggac aacccacatc aacgcccctt ccttcga
//
LOCUS       DAOSOO010000270         3805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24127_length_3805_cov_5.655467, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000270 DAOSOO010000000
VERSION     DAOSOO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_3805_cov_5.655467"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLT86_13620"
     CDS             complement(<1..613)
                     /locus_tag="PLT86_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37695.1"
                     /translation="MAFRVAIIGTGHVAHLHANAYRASGSAEIVAVADVNDEVRAAFQ
                     REYGIPNTYGDYRAMLDSECPDVVSVCVWPHLHPEMTIRAAGTHGVKGILCEKPLALT
                     LPAMNEMIAMCRQKGIALGVGHQLRHQARVRAVRELISSGTFGAVRRVWAVCGSGDLM
                     SNTIHTIDLVHFFCDDRQVTRVFAQVDPGDGAYRFGHPVERTSV"
     gene            834..1502
                     /locus_tag="PLT86_13625"
     CDS             834..1502
                     /locus_tag="PLT86_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT37696.1"
                     /translation="MPCVLIVSNQQNARSAIAEDVEEIGIEAITTDDLSRALDILETR
                     HPSLVLLDLIDRTLGAESLWRQQMRQAPKDLPFLLFIPEEGIRNFDFPPQTKDFILVP
                     FVRAELHARLRLALGSLLSTQASNVIERGQLRIDLDRYKVSIANQPVELTLKEFELLR
                     FLAEHPGKVHTRESLMTRVWGYDYFGGTRTVDVHIRRIRAKLEPHADEYIDTVRGVGY
                     CFRD"
     gene            1511..2845
                     /locus_tag="PLT86_13630"
     CDS             1511..2845
                     /locus_tag="PLT86_13630"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT37697.1"
                     /translation="MEHADASYVVKQAKDHKVKFIRLWFTDVLGFLKSVAIVVDELED
                     ALVDGVGFDGSSIEGFARVDESDMLAVPIPTSFQILPWRPQSDNAVARMFCEVRQPDG
                     TPYEGDPRFVLQRALKRAHDMGFTFYVGPELEFFYFKEDQPPPKVLDRGGYFDLTPLD
                     IASDLRRDTIMTLEQVGIDVEYSHHEGAPSQHEIDLRYADALTMADSIMTTRLVVKEI
                     AGKHGIYASFMPKPLVAFNGSGMHIHMSLFRGDSNAFFDPGNPEHLSATGEQFVAGIL
                     RHAAEFTLVTNQWVNSYKRLKPGFEAPTQIAWAYRNRSHILRVPSIKPGRSESMRIEL
                     RSPDVACNPYLAFAVLLHAGLEGIERQYKAIPPTESSPEQLSASARRRKGIKQLPDSL
                     NEAIRLAEQSELVRRALGPHIHAKLIENKRMEWLKFAEHVTDYELDRYLPLL"
     gene            complement(2945..>3805)
                     /locus_tag="PLT86_13635"
     CDS             complement(2945..>3805)
                     /locus_tag="PLT86_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOT37698.1"
                     /translation="VDDSEVERGFKWLTEMLGKRILYDGKMLTPETLKTQIRLYCAMK
                     RVNEEKGFDFCGLKGQRELTEYLCLGDVPEMIMNDPYDWNGPKEPTVCATEADAYAAM
                     TMQILKYVTGGLPTLFMDVRLYLPEKNLWDWCNSGNHSSWYAAQSSDPKKNYAKIHFH
                     PALEMYFKAGGASVEFDAKPGEMTFCRLGLWDDKPFMVITRGNALDLPAAERKAINKA
                     TNPTWPHVHARIQGSFEEFLSVFPCNHVLGAPGNLVQPLVYLCEIAGIPPIVLGEEGA
                     KRIQPIWERI"
BASE COUNT          805 a         1152 c         1033 g          815 t
ORIGIN      
        1 ctaccgacgt gcgttccacc gggtggccga aacggtacgc accgtctccg ggatcgacct
       61 gcgcaaaaac gcgcgtcacc tggcggtcgt cgcagaagaa atgcaccaga tcgatcgtgt
      121 gaatggtgtt gctcatcagg tccccggacc cgcacacggc ccagacgcgc cggaccgcgc
      181 cgaatgtgcc tgagctgatg agttcccgta ccgctcgaac gcgcgcctgg tgccgcagct
      241 ggtgccccac ccccaaggct atgccctttt gcctgcacat tgcaatcatc tcgttcattg
      301 ctgggagtgt cagcgcaagc ggcttttcac aaaggattcc cttgacaccg tgcgttcccg
      361 ccgccctgat cgtcatttcg ggatgaagat gcggccacac gcacacgctc acgacgtcag
      421 gacactccga gtcgagcatt gctctgtaat cgccgtacgt gttcggaatg ccgtactccc
      481 tctggaatgc cgcgcgaact tcgtcgttta cgtctgcgac tgccacgatc tccgcgctgc
      541 cggaagcacg atatgcgttc gcatggagat gcgcaacgtg ccctgtcccg atgatagcca
      601 ccctgaacgc catcgccact ccttcgtttg tgcgtcgtca taaccactgc cgggaggtgt
      661 ttgaagttca gaaaatctct cttcatcggc aagtgaaccg gtgtcctgca cttctttgcc
      721 gcgcgcaggc gcccttgcac agagtctccg cgagaccgat agtacatatt cgagcgtgac
      781 tactttgaaa tcacgcaaca aatcacgcag ttctgcccag gaacgtgcct cccatgccat
      841 gcgtgctcat tgtcagcaac cagcagaacg ctcgcagtgc gatcgccgaa gatgtcgagg
      901 aaatcggcat cgaggccatc accacggatg acctcagcag agcgctcgac atactcgaaa
      961 cgaggcaccc gagcctcgtg cttcttgatc tgatcgaccg gacactcggc gcggaaagcc
     1021 tctggcgaca gcagatgcga caggcgccga aagacctgcc attcctgctg ttcattccgg
     1081 aagaaggtat tcgcaatttc gatttcccgc cgcagacaaa ggatttcatc ctggttccgt
     1141 tcgtccgggc ggagcttcac gcccggctgc gacttgcgct gggttcattg ttgtcgactc
     1201 aggcatcgaa cgtaatcgaa cgcgggcagc tccggataga cctcgacagg tacaaagtgt
     1261 ccatcgccaa ccagcccgtt gaactcaccc tgaaggaatt cgaactgctc cgtttccttg
     1321 ctgagcaccc gggcaaagtg cacacaagag aatccctgat gactcgcgta tgggggtacg
     1381 actattttgg cggcacacgc accgtggatg ttcacatacg gagaatccgc gcgaaactgg
     1441 aacctcacgc agacgagtat atcgacactg ttcgaggtgt cggatactgt ttccgcgact
     1501 agggacggcc atggaacacg ctgatgcaag ctatgtcgtc aaacaggcga aagaccataa
     1561 ggtcaaattc atccgcctct ggttcaccga cgtcctaggt tttctgaaaa gcgtcgcgat
     1621 cgtcgttgat gagttggaag acgcgctggt tgacggcgtc ggtttcgacg gctcctcgat
     1681 tgaaggcttc gcgcgcgtgg atgagagcga catgctcgcc gttcccattc cgaccagttt
     1741 tcagatactt ccctggcgcc cgcagtctga taatgcggtt gcccgcatgt tttgtgaggt
     1801 ccgccagcct gacgggacgc cgtacgaagg cgatccgcgt ttcgtgcttc agagagctct
     1861 gaaacgtgcg cacgacatgg gattcacatt ctacgtcggt cccgaactcg agttctttta
     1921 cttcaaagag gaccaaccgc cgccgaaggt ccttgacagg ggtgggtatt tcgacctcac
     1981 cccgcttgat atcgcgtccg atttgcgccg cgataccatc atgacgctgg aacaggtggg
     2041 gatcgatgtc gagtacagcc accacgaggg cgcaccatcg cagcacgaaa tagatcttcg
     2101 gtacgcggat gccctcacaa tggccgattc gatcatgacc acgcgccttg tcgtgaaaga
     2161 gatcgcggga aagcatggaa tttacgcgag tttcatgccg aagccgttgg ttgcattcaa
     2221 cggcagcggc atgcacatcc atatgtcgtt gttccgaggg gattccaacg cgtttttcga
     2281 tcccgggaat ccggagcacc tctcagcgac aggagaacag tttgtcgccg gtatcctgcg
     2341 ccatgcggcc gaattcaccc ttgtgacaaa ccaatgggtc aactcgtaca aacgcctcaa
     2401 accaggattt gaggcgccga ctcaaatcgc ctgggcgtac cgcaatcgtt ctcacatcct
     2461 tcgcgttccc tcaatcaaac ccggccgttc ggaaagcatg cgcatcgagt tgcgctcgcc
     2521 ggatgtggca tgtaacccgt accttgcctt cgccgtcctc ctgcacgcag gtctggaagg
     2581 catcgagaga cagtataagg cgattccgcc aaccgaaagt agtccggaac agctctcggc
     2641 ttcggcacgt cgacgtaaag ggatcaaaca gctcccggac agcctgaacg aagccattcg
     2701 gctcgccgaa cagagcgaac tcgtgcgacg cgcgctcgga ccgcacatcc acgcaaaact
     2761 catcgaaaac aagcgcatgg aatggctcaa gttcgcggaa cacgtgaccg attacgaact
     2821 ggacaggtac ctgccgttgc tttgaggctc aagggggaat attcaagtta cgccagctcg
     2881 gaatacacag cgggcgcggt tctcctgaac cgcgcccgcc gtatttttga ccgcgggagg
     2941 gtcttcagat gcgctcccag atgggctgga tccgctttgc accctcttcg ccaagaacga
     3001 ttggcggaat ccccgcgatt tcgcagaggt acaccagagg ctgcacgaga ttgccaggtg
     3061 ccccgagcac gtgattgcac gggaacacgc tgagaaactc ctcgaaactg ccctgaattc
     3121 gcgcgtggac atgcggccac gtcggattgg ttgccttgtt aatcgccttc ctttccgccg
     3181 ctggaaggtc caacgcattg ccgcgggtta tcaccatgaa aggtttgtcg tcccacaatc
     3241 ccaaccggca gaaggtcatt tcacctggtt tcgcgtcaaa ttccaccgac gcgccgcctg
     3301 ccttgaagta catctccaag gcgggatgga agtgtatctt ggcgtagttc ttcttcggat
     3361 cgctggattg cgcggcgtac caactcgaat ggttccccga attgcaccag tcccacaggt
     3421 tcttttccgg caggtagagg cgcacgtcca tgaacagcgt tggaaggccg ccggtgacgt
     3481 acttcagaat ctgcatagtc atcgcagcgt acgcgtcggc ttcggtcgcg cacaccgtcg
     3541 gttccttcgg cccgttccag tcgtagggat cgttcatgat catctccgga acgtcaccaa
     3601 ggcacaggta ttcagtcagt tcgcgctgac ctttcagccc gcagaaatca aacccctttt
     3661 cttcgttcac gcgtttcatc gcgcaataga ggcgtatctg ggttttgagc gtttcagggg
     3721 tcagcatttt tccatcgtag aggatgcgtt tgcccagcat ctcggttagc catttgaagc
     3781 ccctttcgac ctctgagtcg tccac
//
LOCUS       DAOSOO010000271         3783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24302_length_3783_cov_5.945279, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000271 DAOSOO010000000
VERSION     DAOSOO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24302_length_3783_cov_5.945279"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLT86_13640"
     CDS             complement(<1..569)
                     /locus_tag="PLT86_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT37699.1"
                     /translation="MTLRERRKMGAIPVPTKQQAAWQDYEVGMFFHYDLNVFVEEKRP
                     GWDHRHYDDYPGPDVFNPQKLDCEQWMEAAKAVGANYAVLTASHGSGFMLWQSGAYPF
                     GVRQSPWRGGKGDLVKDFVEACRRNGIQPGLYSHLRCNGYWRVDHPGLVNEGKGGDPK
                     RQAAYAKARLTMCRELWGNYGPLAEIWFDG"
     gene            complement(611..1306)
                     /locus_tag="PLT86_13645"
     CDS             complement(611..1306)
                     /locus_tag="PLT86_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37700.1"
                     /translation="MRNIAGADICRPIWSRRSPRLPYYDYSLAGAYFVTVCARGRECL
                     FGDVVNGQMRTNVVGAIVDSVWRDLPTYCPSVETDWYVIMPNHFHGILLLVGSTGDGV
                     GAGSPGPLPDANATGQPREVVGGRARAADEGSPGPNDSRETVTGGETPPLRRPSLVNI
                     MAFFKYETTRRVNAERGTAAGRLWQRSFYDHIIRDEDDLSRAREYITMNPAQWAVDKE
                     NVANAIRGNRKGP"
     gene            complement(1365..3302)
                     /locus_tag="PLT86_13650"
     CDS             complement(1365..3302)
                     /locus_tag="PLT86_13650"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT37701.1"
                     /translation="MPDNAKQFAIAREGKPCATIIVPRAAEPRLLDSVADFVHVIERM
                     AGPTLPVLTDGIQTGNGGEIHIGLTDFVKQSGLLPESLPVNGYRIAVCEEHGQPCLVI
                     AGPTTLGTTHGVAGFLTDDLGVLWGMADPLFEDIPVRRDVAVEAIDRTERPTFGFRTL
                     LGPDLTWARRNRVDDGNRTLPYYGHSHNMHAIMPPDQYRDHPEYFALRGGTRIIADGH
                     FGPQPCMTHPDVIRIGIEAARRYFDENPTARTFSLCPNDTKEFCECPNCAALDEGRPL
                     HRGDKIYSDSYFHWVEAVAKELLKSHPDRHLGTYGYWATVLPPRHIKRLPPNVVVFLT
                     VDSSQYFDPDYEAHDFELLRAWREVADNVAYYDYYALGWFMPRVFHGIAGRTIKRIAE
                     IGVVGFHCEAYTHWAHNGAQFYLASRLGWDTTLDPAKLLDEWYARMFGEAATEIRAFY
                     ECLERRWMEVKRPGRWFQGFDWIFHQFDFWPADARQEAWDLLENAYAAAKTEITRRRV
                     DYVRRGHRSAYLMPVSYERAIALSPDSPTLERDIRLIGAAYGDAINRYRRDIKTDLTY
                     GYHYYIGTTYAEERNFVWWQAFLGDAIKKALAGRPELREKLVAEDTTVAELLRVRESA
                     DAETLRRLGWVYRDLEARRST"
     gene            complement(3323..>3783)
                     /locus_tag="PLT86_13655"
     CDS             complement(3323..>3783)
                     /locus_tag="PLT86_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37702.1"
                     /translation="DVTRQRVAYIRAGHEPAYRVARAFEWAHALTPESTEEGIMRVLD
                     EFELFMTTCFTGVEGDPAYSDTYYRGYRFHERTKWIKLDILRCIDRALAGRPEVRQRL
                     LETNATFSEMNDTARNRRTVGYRRWTNKSMSERGIYPNFIPDQPPYLPME"
BASE COUNT          615 a         1232 c         1197 g          739 t
ORIGIN      
        1 ccatcgaacc agatctccgc gagcgggccg tagttgcccc agagttcccg gcacatggtg
       61 agccgggcct tcgcgtacgc cgcctgccgc ttcgggtcgc cgcccttgcc ctcgttcacg
      121 aggccgggat gatccacccg ccagtagccg ttgcagcgaa ggtgcgagta gagccccggc
      181 tggattccgt tccggcggca ggcttcgacg aagtccttca ccagatcgcc tttgccgccc
      241 cgccacgggg attgtcggac accgaagggg tacgcgccac tctgccacag catgaacccg
      301 gagccgtggg aggccgtcag cacggcgtag ttcgcgccga cggcctttgc ggcctccatc
      361 cactgctcgc aatccagctt ttgcgggttg aacacgtcgg ggccggggta gtcgtcgtag
      421 tggcgatggt cccaccccgg ccgtttctcc tccacgaaca cgttgagatc gtagtggaag
      481 aacatgccga cctcgtaatc ctgccacgcg gcctgctgct tcgtcggaac gggaatcgcg
      541 cccatttttc gtctctcccg gagagtcatt cgggccgggc aatccttcgt ccgcggcacg
      601 gacgcggccc ctacggcccc tttcgattgc cccggatggc gttggcgacg ttctccttgt
      661 ccacggccca ctgcgcgggg ttcatcgtga tgtattcccg cgcccgagag agatcgtcct
      721 cgtcgcggat gatgtgatcg taaaatgagc gttgccagag ccggccggcc gccgttcccc
      781 gttccgcgtt gacgcgtctg gttgtttcgt atttgaaaaa tgccatgata ttcaccaacg
      841 acgggcgccg taggggcggg gtctccccgc ccgttacggt ttcgcgactg tcgttcgggc
      901 cgggggaccc ttcgtccgcg gcgcgggcgc ggcccccgac tacctctcgc ggttgccccg
      961 ttgcgttggc gtcgggcagc gggccgggtg acccggcccc tacgccatca cccgtgctac
     1021 ccaccaacag gaggattcca tggaaatggt tcggcatgat cacataccag tcggtctcaa
     1081 cactggggca atacgtgggc agatcccgcc aaactgaatc cacaatcgcc ccaacgacgt
     1141 tcgtccgcat ctgtccgtta acgacatccc cgaacaggca ttcacggccc cgtgcgcaca
     1201 cggtaacgaa ataggctcca gcgagggagt agtcgtaata tggcaagcgg ggggagcgac
     1261 ggctccagat gggccggcat atatccgcac ccgctatgtt gcgcatcacg gcttcctcaa
     1321 cgcccgctga tttcccaccg ttctactcct gtggcgggcc cgcgctacgt gctccgtctg
     1381 gcttcgagat cgcggtacac ccatccgagg cggcgaagcg tttccgcgtc ggcgctctcg
     1441 cgaacgcgca gcagttcggc aacggtggtg tcctccgcca cgagcttctc gcgcagttcc
     1501 ggcctgcccg ccagcgcctt tttgatggcg tccccgagga acgcctgcca ccagacgaag
     1561 ttgcgctctt cggcgtacgt cgtgccgatg taataatgat agccgtaggt gaggtcggtt
     1621 ttgatgtcgc gccggtagcg gttgatggcg tcgccatacg ccgcgccgat gaggcggata
     1681 tcgcgctcga gcgtgggcga atccggcgag agtgctatcg cccgttcgta cgagaccggc
     1741 atgaggtacg ccgagcggtg cccgcggcgg acgtaatcca cgcggcggcg ggtgatttcg
     1801 gtcttcgcgg ccgcgtatgc gttttcgagc agatcccacg cctcctgccg ggcgtccgca
     1861 ggccagaaat cgaactggtg gaaaatccag tcgaatccct ggaaccacct gcccgggcgc
     1921 ttcacctcca tccagcggcg ttccaggcac tcataaaaag cccgaatctc ggttgccgct
     1981 tcgccgaaca tccgggcgta ccactcgtcc agcagtttcg ccgggtcgag tgtggtatcc
     2041 cacccgaggc gggaggcaag gtagaactgc gcgccgttgt gtgcccagtg cgtgtacgct
     2101 tcgcagtgga agcccaccac gccgatttcg gcgatgcgct tgatcgttcg cccggcgata
     2161 ccgtggaata cgcgcggcat gaaccacccg agcgcgtaat agtcgtagta cgcgacgtta
     2221 tccgccactt cacgccacgc gcgaagcagc tcgaaatcgt gcgcctcgta gtcggggtcg
     2281 aagtactggc tggaatccac cgtgaggaac accaccacgt tcggcggcag cctcttgatg
     2341 tgccgcgggg gcaatacggt cgcccagtag ccgtacgtgc cgaggtgcct gtccggatgg
     2401 ctcttgagaa gctccttcgc caccgcctcc acccagtgga agtagctgtc ggagtagatc
     2461 ttgtccccgc ggtgcagcgg gcggccctcg tcgagcgccg cgcagttcgg gcactcgcag
     2521 aactctttcg tgtcgttcgg gcagaggctg aatgtgcgcg cagtcgggtt ctcgtcgaag
     2581 taccgccgcg ccgcttcgat tccgatccgg atgacgtcgg ggtgcgtcat acagggttgc
     2641 ggcccgaaat gtccgtcggc gatgatccgt gtgccgcccc gcagcgcgaa gtactcgggg
     2701 tgatcgcggt actgatcggg cggcatgatg gcgtgcatgt tgtggctgtg gccgtagtac
     2761 ggcagcgtgc ggttgccgtc gtccacgcga ttccgccgcg cccaggtcag gtccggcccc
     2821 agcagcgtgc ggaacccgaa tgtcgggcgt tcggtgcggt cgatcgcttc gacagccaca
     2881 tccctgcgca ccggaatgtc ctcgaacagc gggtcggcca tgccccacag cacgccgagg
     2941 tcgtccgtga ggaagcctgc cacgccgtga gtggtgccca gcgtcgtcgg cccggcgatc
     3001 accaggcacg gctgaccgtg ctcctcgcac actgcgatgc ggtagccgtt gaccggcagg
     3061 gattccggca ggagccccga ttgcttcacg aaatccgtca ggccgatatg gatttcccca
     3121 ccgttgcccg tctggatgcc gtcggtcagc accggcagcg tcggcccggc catacgttcg
     3181 atcacgtgca cgaaatcggc aaccgaatcg agcaggcggg gctcggcggc acggggcacg
     3241 atgatggtgg cgcatggctt cccctcgcgc gctatggcaa actgctttgc gttgtctggc
     3301 atgacatgtc cttcctgtgc cgttattcca ttggcagata cggtggctga tcgggtatga
     3361 aattcgggta gatgccgcgt tcgctcatgg atttgttcgt ccaccggcgg taccccacgg
     3421 tgcgtcggtt ccgtgcggta tcgttcattt cgctgaaggt ggcgttggtt tccagaagtc
     3481 tctgacgcac ctccggccgg ccggccagtg cacggtcaat acagcgcaaa atatccagct
     3541 tgatccattt cgtgcgctcg tggaaacggt aaccgcgata gtaggtgtcc gagtaggccg
     3601 ggtcgccctc aacaccggtg aagcacgtcg tcatgaacaa ttcgaattcg tcgagaacgc
     3661 gcatgatccc ttcttcggtg ctttccggag tcagcgcatg cgcccactcg aatgcgcggg
     3721 ccacgcggta ggccggttcg tgcccggcgc ggatgtacgc cactctctgc cgggtgacat
     3781 cgt
//
LOCUS       DAOSOO010000272         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24317_length_3781_cov_5.571659, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000272 DAOSOO010000000
VERSION     DAOSOO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24317_length_3781_cov_5.571659"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..2045)
                     /gene="ligA"
                     /locus_tag="PLT86_13660"
     CDS             complement(51..2045)
                     /gene="ligA"
                     /locus_tag="PLT86_13660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT37703.1"
                     /translation="MERAEYDRLVDEILEHDYRYYVLADPAISDEEYDGMLRQLRAVE
                     AEHPEWRRSDSPTHRVGGVPTKEFPPVVHQRPMLSLDNTYDETELAEFDRRIRDALGL
                     EDVEYVCELKMDGVALSLLYQGGVLVQASTRGDGVTGDDITDNARTIRTIPLRLRHGL
                     SDVEVRGEVYLPTAEFRRMNEARIEAGEKAFANPRNAAAGTLKLMDPKEVARRPLAFV
                     AYWLDDRQNDGGTQWHALELLQNLGFVVSEHRRLCRSFSEVLLFCEEWEEKRDALPYE
                     IDGVVVKVNRLDFQEQLGYTSKSPRFMIAYKFRARKAETTLLEIGLQVGRTGAVTPVA
                     ILEPVLVGGITISRATLHNADEIRRKDVRPGDTVIVERGGDVIPKVSGVVLEKRPPTS
                     VPFEFPTACPVCGAILFRGAEDAITRCENAACPAQVRGRILHFASRPAMDIEGLGPAV
                     VDQLVNAGLVHNAADLYELEPGRVAALERMGEKSATNLVAAIQASKSRPFPAFVYALG
                     IRNVGETTARALCRRFRSIDTMMSASVESFMEVEDVGPVVAESIVDFFRVEENRRLVE
                     RLKAHGLQTESKENQASEAPPPGKFTGMTFVLTGALERFTRDDAAERIRAKGGTVTDS
                     VSRKTSIVVAGPGAGSKLAKATQLGIEIWDESRFLAELGE"
     gene            complement(2062..2823)
                     /locus_tag="PLT86_13665"
     CDS             complement(2062..2823)
                     /locus_tag="PLT86_13665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT37704.1"
                     /translation="MPRRDSWSKEFQKMVVLGESTVEGGWWTASASERWADILANLIN
                     QCQRTPMQYVNKGIGANAISPRSPGYAASRKPSALERYAEDVVAQKPDLFILCYGLND
                     MRAGMPLNDFIDDMRTIVTDVRNACAPLIILTTIYYMTGWKSYPPYDRGSVALTEEFN
                     RAIRALAEEQQCLVADVWDAEGQADWLIHPDGVHANKVGNILIANRVFETIAKNASAL
                     SNSVHERDLPGRVAQELIRVRESQGDPYRAWWQKH"
     gene            complement(2842..>3781)
                     /locus_tag="PLT86_13670"
     CDS             complement(2842..>3781)
                     /locus_tag="PLT86_13670"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT37705.1"
                     /translation="DEAYREEDERILDAWKTIAEHVCLYEYTNLGWFLPRNFASLTAK
                     RIRYLSQIGIDGYYAEAHPHPAQMAPVMYLTAKLAWDPALNAEDVLDEWYQRMFREAA
                     PHMKGFYVTLDSAWMHADRRGAWFAGLNRMWEEFAAYPPRYREVAWRHLDAAMRSARD
                     ETTRMRVQYVREGHEPAYRVSRAFEWAHSLNTRSSEDEIVRVLSEFERFMETFFRNVE
                     SNPDYADIAYRGARFHERTKWIKLDILRCIDRALAGRPEMHARLLATNATFSEMDDTA
                     KNRRTRGYRRWTNRDMETRGVFPSYIADQPPYLPSE"
BASE COUNT          730 a         1222 c          990 g          839 t
ORIGIN      
        1 ttgttcatac agcgaacgtt gtggccggtc gggaatccac taaaggggca tcattccccc
       61 agttccgcca gaaaacgcga ttcgtcccag atctcgattc caagttgcgt tgctttcgcc
      121 agcttggagc ccgcaccggg tcccgccaca acaatggagg tttttcggct cactgagtcc
      181 gtcactgttc ctccttttgc acggatacgt tccgccgcat cgtcgcgcgt gaatcgttcc
      241 agcgcacccg taagaacaaa cgtcatcccc gtgaactttc ccggtggcgg tgcctccgat
      301 gcctgattct ccttggattc cgtctgcaga ccatgggctt tcaaccgttc tacaagtctg
      361 cggttctcct ctacgcggaa gaagtccacg atactctctg ccacaacggg tccgacatcc
      421 tctacttcca tgaacgattc caccgaagcg ctcatcattg tatcgatgga acggaaacgc
      481 cggcagagcg cccgtgccgt ggtttcaccc acatttcgga taccgagagc atagacaaac
      541 gcgggaaacg gtcgcgattt tgaagcctga atcgcggcaa cgaggttcgt ggccgatttc
      601 tctcccatgc gctccagtgc cgctacccgc ccgggttcaa gctcatacag gtctgcggcg
      661 ttgtgaacca ggcccgcatt caccagctga tccaccaccg ccggccccag cccctcgatg
      721 tccatcgccg gccggctggc gaaatggaga atccttccgc gaacctgcgc cgggcacgcc
      781 gcattctcac atcgcgtgat cgcatcctcc gctccacgaa acagaattgc gccacacacc
      841 gggcacgcgg tcggaaattc aaaaggaacc gatgtcggtg gccgtttttc gaggaccacg
      901 ccgctgactt tcgggatcac gtccccgcct cgttccacga ttacggtgtc gccgggccgg
      961 acatccttgc gccgtatttc gtcggcattg tgcagtgtcg cccggctgat cgttatcccg
     1021 ccgacgagaa ccggttccag aatcgccacg ggtgtcaccg ctccggttcg gccaacctgc
     1081 agtccgattt cgaggagagt cgtttcggct tttcgcgccc gaaatttgta cgcgatcatg
     1141 aagcgcggac tcttggacgt gtagccaagc tgttcctgaa aatccagccg gttcaccttg
     1201 accacgacgc cgtctatttc gtagggcagg gcgtcgcgtt tctcctccca ttcttcgcag
     1261 aacagaagca cttcggagaa cgacctgcac agccgccgat gctcacttac aacgaaaccc
     1321 agattctgaa gtaattcgag cgcgtgccac tgcgtgccac catcattttg ccggtcatcc
     1381 agccagtacg caacaaaggc gagcggacgg cgtgccacct ccttcgggtc cataagtttg
     1441 agagttcctg cggcagcgtt acgcggattc gcgaaggctt tttcgccggc ctcgatgcga
     1501 gcttcattca tccttcgaaa ttcagccgtc ggcagataca cttctccacg cacttccaca
     1561 tcgctcagcc cgtgccgcag gcgaagaggt atggtgcgga tggttctggc gttgtccgta
     1621 atatcgtctc ccgtaacccc gtcgccccgg gtggaggcct gaacaagcac acccccttga
     1681 tacagcaggc tcagagcaac cccgtccatc ttcagttcgc agacatactc cacgtcctcc
     1741 aatcccagag catcacggat gcggcggtcg aattccgcga gttctgtttc atcatatgtg
     1801 ttgtcgaggc tcaacatggg gcgctgatga acaacgggcg gaaactcctt tgtcggcacc
     1861 cctcccacac gatgtgtcgg cgagtcggat cgccgccatt ccgggtgttc cgcttccaca
     1921 gcccgcagct ggcgaagcat gccgtcgtat tcctcatcac tgatcgcagg atccgctaga
     1981 acatagtaac gatagtcgtg ttcgagaatc tcgtcgacga gccgatcgta ttccgcgcgc
     2041 tccataccgc catcccccgt ctcagtgctt ctgccaccac gcccgatagg gatcaccctg
     2101 cgactcccgg acccgaatga gttcctgcgc cacccttccg ggcaggtctc gttcatgcac
     2161 cgaattgctc aaggcgcttg cgttcttggc tatcgtttca aagacgcggt tcgcgatcag
     2221 aatgttgccg accttgttcg cgtgaactcc atccggatgg atcaaccaat cggcctgtcc
     2281 ctccgcgtcc cacacgtcgg caacgaggca ctgctgctct tccgccagcg ctctgatcgc
     2341 ccggttgaac tcctcggtga gtgccaccga cccccgatca tacggtgggt agctcttcca
     2401 gccggtcatg tagtaaatgg tcgtgaggat gataagcggg gcgcacgcgt tcctgacgtc
     2461 cgtcacgatg gtgcgcatgt catcaatgaa atcgttcaac ggcattccgg cgcgcatgtc
     2521 gttaaggccg taacacagga tgaacaggtc cggcttctgg gcgacgacgt cctccgcgta
     2581 ccgttccagc gcgctcggtt tgcgcgaggc agcatacccg gggcttcgcg gactgatcgc
     2641 atttgcccct atccctttgt tgacatactg catcggagtt cgctggcact gattgatcag
     2701 gttcgccaga atgtccgccc agcgctctga agcggatgcc gtccaccacc caccttccac
     2761 ggtgctttcg ccaagcacaa ccattttctg gaactctttg ctccacgagt cacgacgtgg
     2821 catatctacc tccaatgcag gtcattccga cggaagatac ggtggttggt ccgcgatata
     2881 tgacggaaaa acgcctcgcg tctccatgtc gcggtttgtc caccggcggt aacctcgggt
     2941 tcgacggttc tttgcagtgt cgtccatctc gctgaacgtc gcgtttgtcg cgagaagccg
     3001 tgcatgcatc tcggggcgac ccgccagcgc tctgtcaatg caccgcagta tatccagctt
     3061 gatccatttc gtgcgttcgt gaaaccgtgc gccgcggtag gcaatgtccg cgtaatcggg
     3121 attagactcg acgttccgga aaaacgtctc catgaacctt tcgaactcgg agagcacacg
     3181 gacgatctca tcttcagaac ttcgcgtgtt caacgaatgc gcccattcaa acgcccggga
     3241 aacacggtat gcaggttcgt gcccctcgcg aacgtattgc accctcatgc gggtcgtctc
     3301 atccctcgcc gagcgcattg ctgcatctag gtgccgccat gccacttccc tgtaccgggg
     3361 aggatatgcg gcaaattcct cccacatacg attgagaccc gcaaaccatg ctccgcgtcg
     3421 atccgcgtgc atccacgcgc tgtcaagggt aacatagaag cctttcatgt gtggagccgc
     3481 ttcacggaac atcctctggt accactcgtc cagaacatct tcggcattca acgccgggtc
     3541 ccaggcgagc ttcgccgtca ggtacattac cggcgccatt tgtgcgggat gcggatgcgc
     3601 ctcggcgtag tatccatcta ttccgatttg gctcagataa cgaatcctct tcgcggtgag
     3661 gctcgcgaaa ttccgcggaa ggaaccagcc aagattcgtg tactcgtaaa ggcagacatg
     3721 ctcggctatg gtcttccacg cgtccagtat gcgttcgtct tcctcgcggt acgcctcgtc
     3781 g
//
LOCUS       DAOSOO010000273         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24693_length_3736_cov_4.785928, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000273 DAOSOO010000000
VERSION     DAOSOO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24693_length_3736_cov_4.785928"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1166
                     /locus_tag="PLT86_13675"
     CDS             90..1166
                     /locus_tag="PLT86_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37706.1"
                     /translation="MGAVRIGVLGGGGILGSHAPAYAKIRDLCEVVAVAEPDVKRHDR
                     VKALLGADVRLYADYRDVIGLADVDAVDIVLPHYLHLPATLCAAEKRKHVLVEKVMAR
                     NVCECDQMIDACDKAGVSLTVCHDRRYDAKWIALKEVVDSGALGEIFYWKLEHNQNVV
                     FPEGSWVRSREGLGGGAIMSCLTHQIDSLRWYGGEFARVSCMTRVIPERMEGETVGVI
                     IGKMKSGALAQLSINWFTRSNGARPNDLWYELVHVCGTRGEAYFMSSRGTFVMLHDAS
                     DAAAVSRYGREALDGFAEVPSGTWTGHERCIAEWVKSLRGEPSQVITSGRDVRGTVEV
                     AEAAYRSAQCGREIELPINPVPGE"
     gene            1219..2238
                     /locus_tag="PLT86_13680"
     CDS             1219..2238
                     /locus_tag="PLT86_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT37707.1"
                     /translation="MRVGVDTFTIRELKLDPFATFDYLVKRGFDGAQFGGLRSLSKEL
                     DAAMLREVHSEADRRGLYCHVSVPCCNPHIVAGDLAEHIAELTKQIEAAAEAGWHEFH
                     STLGNGTERYEHPVRWTQQLADSADFVRNLGPVLRQNGSRINLETHGDVTTFELVRLI
                     EDVGPDIAGICLDTANVLCHAEDPVLAARRAAPYTHLTHTKDGIIYFSRQGYVRQGRP
                     PGQGCLYWSQILPALAEFSPDLPLSIEDHKWLFEFNVFEKRWLALHPDLTLEEFARVM
                     RLAHLCEERIACGELENPDRYEAIPYAEQMEERLAFGRDFLNALLVARGLKTKKGAEQ
                     SSAPS"
     gene            complement(2255..2986)
                     /locus_tag="PLT86_13685"
     CDS             complement(2255..2986)
                     /locus_tag="PLT86_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT37708.1"
                     /translation="MGLDKRAIASSLAPYPDDLALTERVAATHPEVVAILEDFVVRRP
                     HLAYLAPTLCRVICALIRCYDRGGTLFLCGNGGSFADSVHIAGELLKSFERDRSLTSE
                     QKAKFAGLPDAEALTTHLEQGLRAYALGNSNPLATAIQNDNKLRDIHYAQDLFAMGRP
                     GDVLLGISTSGNATNVLYALQVGKALGMTTIGMTGENGGKLAAMADIAIKAPGTITKY
                     VQEDHFPTYHAICAMIEAYYFPKPR"
     gene            complement(3016..>3736)
                     /locus_tag="PLT86_13690"
     CDS             complement(3016..>3736)
                     /locus_tag="PLT86_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37709.1"
                     /translation="LCGNFASAIHDFELALIGETSEVVPNALPRGEFGLAAETGFYIN
                     DAMKWGNDRKLGLGETLGRVINGEAAQELKMEFPWKHESYLAQGYLKGVPVTIHATMG
                     TDITDQHPNFDGECKGGCSGRDFGVFAAHVLEMHRKGGGVYLNVGSAVTGPEVFLKAV
                     SMAANVGEPPTGLVTADFDIRPANLEHMQDEHKHSYYFRDVKSVVTRIPEAFGGKGHY
                     IQGDFFKAIPALYQEIRKRMG"
BASE COUNT          769 a         1063 c         1117 g          787 t
ORIGIN      
        1 catgttgctg ccgacgaagc ccgttgaacg cggactttcg ctgcccggat cgccggggca
       61 cacgcacgaa tctgaacgga gtatgacgta tgggcgctgt tcgaatcgga gttcttggag
      121 ggggcggtat ccttgggtct catgcgcccg cgtacgcgaa aatcagagat ctctgcgagg
      181 tcgttgcggt cgccgagccc gatgtgaaac ggcacgaccg tgtgaaggcg ctcctaggcg
      241 ccgacgtcag actttacgcg gattaccgtg acgtgatcgg tctggcggat gttgacgcag
      301 ttgatatcgt gctcccccat tatctgcatc ttcccgcaac cctctgcgcg gcggagaagc
      361 gcaagcacgt tcttgtggaa aaggtgatgg cgaggaacgt gtgcgaatgc gaccagatga
      421 tcgacgcgtg tgataaggcc ggtgtctcgc tgaccgtatg ccacgaccgc cgctacgacg
      481 caaaatggat tgccttgaaa gaggtggtgg attcgggggc cctaggggag attttctact
      541 ggaaacttga gcacaaccag aacgtggtat tcccggaagg aagctgggtc cgttcgcggg
      601 agggactcgg gggcggcgcg attatgagct gcctgacgca ccagatagac tccctccgtt
      661 ggtacggcgg cgagttcgca cgggtgagct gcatgacgcg agtgattccc gaaagaatgg
      721 aaggcgagac cgttggtgtc atcatcggaa aaatgaaatc aggtgcgctt gcgcagcttt
      781 caatcaactg gttcacgcga tctaacggcg cgcgtccgaa cgacctttgg tacgaactgg
      841 tccacgtatg cggaacgagg ggcgaagcgt acttcatgtc gagccgcgga acgttcgtga
      901 tgcttcatga cgcatcggac gccgccgcag tatccagata cggtcgggag gctctggacg
      961 ggtttgctga agttccgagt ggaacttgga ccggacacga acggtgcatt gcggaatggg
     1021 tgaagagcct ccgcggcgag ccttcgcagg tgattacgag tggccgcgac gtgcgcggca
     1081 ccgttgaggt agcggaagcg gcgtaccgct ccgcgcagtg cggcagagag atcgagctcc
     1141 ccatcaaccc tgtgcccggc gaatgaccag actgcatacg gcgagtaagg cgttgcctcg
     1201 ggatcgtgga gtgagaccat gcgcgtaggt gtggacacgt tcaccattcg agaactgaaa
     1261 cttgatccct tcgcgacctt tgactacctg gtcaaacgtg gatttgacgg ggcgcagttt
     1321 ggaggactcc gaagcctcag caaagagctt gacgcagcca tgttgcgtga ggtacacagc
     1381 gaagcggacc gccgggggct gtattgccat gtctcggtgc catgctgcaa ccctcacatt
     1441 gtcgcgggag acctggctga acacatcgcg gaattgacga agcagatcga agccgccgcg
     1501 gaagcagggt ggcatgagtt ccactccacc ctcggaaacg ggacggaacg atacgagcat
     1561 cccgttcgct ggacacaaca actcgccgat tcggcggact ttgtccgaaa tctgggcccg
     1621 gttcttcgcc agaatggaag caggatcaat ctggaaacac acggggacgt tactacgttc
     1681 gaactggtgc gccttattga agacgtgggg ccggacattg cgggaatatg tctcgacacg
     1741 gcgaacgttc tttgtcacgc ggaagaccct gtgctcgcag cgcgccgtgc cgctccctat
     1801 actcatctca ctcacaccaa agacgggatt atctattttt cccgccaagg gtacgtgaga
     1861 caaggacgcc caccgggaca aggctgcctt tattggagtc agatactgcc tgcgttggcg
     1921 gagttctccc ctgatctgcc cctctcaatc gaggaccaca aatggctctt cgagttcaac
     1981 gtttttgaga aacgctggct cgcgctgcat ccggacctca ctctcgagga gttcgcacgg
     2041 gtaatgcgac tcgctcacct gtgcgaggaa cggatcgcct gcggagaatt ggagaacccg
     2101 gaccgatacg aagcaattcc ttatgcagaa caaatggagg aacgactagc gttcggcagg
     2161 gatttcctga acgcgttgct ggtggcaaga ggcttgaaga cgaagaaagg cgcggaacag
     2221 agctccgcgc cttcttgatc gcctgcgccc tgtttcaacg gggtttcggg aagtagtacg
     2281 cctcaatcat ggcgcagatc gcgtggtatg tcggaaaatg atcctcctgg acgtacttcg
     2341 tgatggtccc cggcgccttg atggcgatgt cagccatggc cgcgagtttc ccgccatttt
     2401 ccccggtcat tccgatggtc gtcataccca gcgctttgcc tacctgaagc gcgtacagca
     2461 cgttggttgc atttccgctc gttgagatcc cgagaagcac gtcccccggg cgtcccatcg
     2521 cgaacaggtc ctgagcgtag tggatatcgc gcagtttgtt gtcgttttga attgccgtag
     2581 cgagcggatt gctgttgccg agcgcatacg ctctgaggcc ctgctcgaga tgagttgtca
     2641 gtgcctcggc gtcgggcaga ccggcgaatt tcgccttttg ctcggaagtc agagaacgat
     2701 cccgttcaaa cgatttcaac agctcgccgg caatgtgaac ggaatccgca aaactccctc
     2761 cgttcccaca caggaaaagt gtcccgcccc tgtcataaca ccgtatgagc gcgcatatga
     2821 cccgacaaag cgtcggtgcg aggtatgcga gatgcggacg ccgcacaacg aagtcctcaa
     2881 gaattgccac cacttcgggg tgggtcgccg ccactcgttc cgttagcgcg aggtcatccg
     2941 gatagggagc aaggctgctc gcgatagctc gtttgtccaa tcccattttc gcctctcaat
     3001 cggtcgaaaa actgactagc ccatgcgttt ccggatttcc tgatacaacg cgggaatcgc
     3061 cttgaagaag tcgccctgga tgtaatgtcc cttgcccccg aaggcctcgg ggattcttgt
     3121 cacaacgctc ttcacgtctc tgaagtagta actgtgtttg tgctcatcct gcatatgttc
     3181 gaggttggcg ggacgaatgt cgaaatcagc cgttacgagt cccgtcgggg gctcgcccac
     3241 gtttgccgcc atgctgactg ccttcaggaa gacttccggc cccgtcacgg cagaaccgac
     3301 gttgagatac actccgccgc ctttccggtg catttcgagg acgtgggcgg cgaaaacgcc
     3361 gaagtcccgt ccactgcatc cccctttgca ctcaccgtcg aaattcgggt gctggtcggt
     3421 gatatccgta cccatcgtcg catggatggt cactggaact cccttgagat acccctgcgc
     3481 aaggtaactc tcatgcttcc acgggaattc cattttcagt tcctgcgccg cttcgccgtt
     3541 gatcacgcgg ccgagcgttt ccccaagtcc aagtttgcgg tcgttgcccc atttcatcgc
     3601 atcgttgata tagaagcccg tttcggcggc gagaccgaac tctccccgtg gcagcgcgtt
     3661 cggcacgact tccgaagtct cgccgatcag cgccagctcg aaatcatgga tcgcactcgc
     3721 gaaattgccg cacagg
//
LOCUS       DAOSOO010000274         3707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_24926_length_3707_cov_4.658543, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000274 DAOSOO010000000
VERSION     DAOSOO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24926_length_3707_cov_4.658543"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLT86_13695"
     CDS             <1..236
                     /locus_tag="PLT86_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT37710.1"
                     /translation="KRWLAENLDKIELFRLPPYSPEFNPVEGIWKTTRKMTTHNCFYP
                     TVPERDAALCHTFETFRENPELIDNQVARRRNP"
     gene            556..1686
                     /locus_tag="PLT86_13700"
     CDS             556..1686
                     /locus_tag="PLT86_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37711.1"
                     /translation="MARICILVLAHAASVIAAVGVSACGSDSDDGSEGGAKAVQPLEA
                     PLSYVDAFGRTWLRAEKPVVYAEGSKDDHIERGPEPAAVVDIAEVSLDEATIALRPKA
                     LRGDWEYTLCDEDAREFAREIQESARNGVPITTGIPGATADEETSANDGGDPSAPTQI
                     VSGDDRFSIEGMKAYYPYNNVAVMDNAGTCTAFKLVNHHTAVTAAHCVHTGSTWKFRK
                     RITFRPRSTVGTTCYGMTVPGCWDGDNRQCDYATIKFREGSGYCNFDTYNVGYLGWVP
                     TYWWEGSMYHSLWGYPSEQMKQEWNYPEMVWDYNTMYGLYVGNNISHSTDATGGQSGS
                     PLYRGGAYATGIYWGFYNGGSDNRAVGITQAIYDWMVANGGY"
     gene            2993..3403
                     /locus_tag="PLT86_13705"
     CDS             2993..3403
                     /locus_tag="PLT86_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37712.1"
                     /translation="MKIRFALLVMGACCGACGEQDEACTSMGCFDGVEVRLAAPLSAS
                     ELFVHVQFDDKDVRCEFKTGVDTCKDEGVSLDGDIGHVYGFRLAGRHPDTVTFSFSDL
                     NQVLVSATVNPKFVTQQPNGPNCTPTCDNGFVQL"
BASE COUNT          745 a         1007 c         1175 g          780 t
ORIGIN      
        1 ggaagaggtg gttagcggaa aacctcgata agatcgagct tttccgactt ccgccgtata
       61 gtccagaatt caatccggtg gagggcatat ggaaaacgac ccgcaaaatg acgacgcaca
      121 attgtttcta tccgactgtc ccagaacgcg acgcagctct ttgccatacc ttcgaaacgt
      181 ttcgtgagaa cccggaactt atcgacaatc aggtggcgcg caggcgcaat ccgtaagtca
      241 gagcgaaacc catcgttacg gttgaatcgg cgatgctcgg tatggacagt tacgtctgaa
      301 ttgcaagcag aaagcacaaa tcggcaccta ttttagggtt atgcgctcat tatctcccgt
      361 tcgcctgaac atggcctctt ctgatcctcg attcccgcca acttgtgact catgaggctc
      421 catgtccatg tgaagctgta tagacaagtc gtgatcaaca aaaatattga cggaacgaga
      481 cggccatgtc accgttggaa tgtcagtgtt tcgcgtggtt ctgaaccgtt cgctttggca
      541 gcgcaggagg gctagatggc taggatttgc attctcgtac ttgcgcatgc agcctcggta
      601 atcgcggccg tcggtgtgtc ggcttgtgga agcgactctg atgatggatc cgaaggaggg
      661 gccaaggcgg tccaaccact cgaggcgccg ttatcctatg ttgacgcatt tgggcgcaca
      721 tggctgaggg ctgagaagcc ggtggtgtac gccgagggtt cgaaagatga tcacatcgaa
      781 cgcgggccgg aacctgcggc ggtagttgat attgctgagg tgtcgctgga tgaagcaact
      841 atcgcgctca ggccgaaggc acttcgggga gactgggagt atacgttgtg cgacgaggat
      901 gcgagagaat tcgcgcggga aatccaggag tcagcgcgga atggtgtgcc gatcacgacc
      961 gggatacctg gagcgactgc tgatgaggag acatcggcga acgatggcgg cgatccgtcc
     1021 gctccaactc agatcgtcag cggtgacgat aggttctcaa tagaggggat gaaggcgtac
     1081 tacccgtaca acaacgttgc ggtgatggac aacgcaggta cgtgtacggc cttcaagctg
     1141 gtaaaccatc atactgcagt aactgccgcg cactgtgttc atacaggtag cacgtggaag
     1201 ttcaggaaaa ggatcacttt tcgtcctcgt tcaaccgtcg ggacaacctg ctacggcatg
     1261 acggttcctg gttgttggga tggcgacaat aggcagtgcg actacgcgac gatcaagttc
     1321 cgtgagggat cgggatactg caattttgac acgtacaacg tcggatacct gggatgggtc
     1381 ccgacctact ggtgggaagg gtcgatgtac cactccttgt ggggatatcc gtccgagcag
     1441 atgaagcaag agtggaacta ccccgagatg gtgtgggact acaacacgat gtacggcctc
     1501 tacgtgggaa acaatatctc gcattcgaca gacgcgacgg gaggtcaaag tggttcaccg
     1561 ctctatcgcg gcggggccta cgctaccggg atctactggg gcttctacaa cggcggttct
     1621 gacaacaggg ccgttggaat aacacaggcg atctatgact ggatggttgc caacggtggg
     1681 tactaggcga cgtgaagaac tcgccgctga cgcccctact ggcccttata gctgtctccg
     1741 tcggttgctc agagggagat gaagcgatgc tatcttcatg ccgtaccgac gaactccttg
     1801 gcggggtgcc agttagtacc gagcccggcg tggttagcgt cggagaccac tgtagcggcg
     1861 tgttggtgca tccctatgtc gttctcttcg ccgctcactg tggcacggca gttgaggaag
     1921 tgagcatagg cgccatgtca taccgcaccg agatctgcga ggctgcgccc tctggtggca
     1981 gcgttgctgg cgatttggca tattgcttct tgaagtcgcc cgcggcgtct gaagccgtcg
     2041 caattccggc caccggttgc gaggcggacg ctatccgaga gggcatggag attactgtgc
     2101 tgggacgcgg gctcccgacc atggaggaac aggaagctca ggtcattgtg caggaatccc
     2161 aggacagctt ggtcgtagcc ggccaaggtg tagcggcgtg tccgggggac tccgggggtc
     2221 ctgttttggc acgcttgtcg agcggggacc agacggccca gagactgatt gggacaatct
     2281 cgcgaggcag tcgcacatgc accagggagg atgggcggat ccacgtgact cctgtcttcc
     2341 cactcgtccc gtggttggag tcgcgcacgg ggctagatct caccccgtgt gggagcgcca
     2401 acggagtctg ggacccttcg cccacgtgtc ggggggatga tggtgcgctc tgggattact
     2461 gcggagcgcc tgccatcata gtggcgggcg acgtatctcc gcccaacctg gcgattggtc
     2521 ttgacgcgaa cgagtctgga gggttgttcg atctgagtgt tgaattggac gtgtcagacc
     2581 tcgagtcggg cgtgcgggaa acggacttgt tcgtctacga tcgggcgggc ggcgttctct
     2641 ggagcagact acgttccata gcgccctaca cgttttcgcc cagacgcctg cgccctggca
     2701 cctatgtgct tgttgcgcgg gcatccgact tctctgggaa cgagaccaag atggcacggg
     2761 aggtggcctt ggatgccgag gtctcaagcg ctggacgctg caacacccgc ccaggccgct
     2821 tctggttctc gaatgttgcg tcgctcgttg cggggatggt tttgctcgtg tcggtttttc
     2881 gagcgcggcc gagatcgcgc ggcagggggc gatggaagga cccacgggtt gacgcgtcct
     2941 gtgccgatcg ccgacgcgcc tgcgtgcgcc cagtgggcaa aaggaggaaa tgatgaagat
     3001 tcgatttgcg cttttggtga tgggggcttg ctgcggcgcc tgcggagagc aggacgaggc
     3061 atgcacatcc atgggctgct tcgacggcgt cgaggtgcgt ttggctgccc cgctctcggc
     3121 atctgagctg ttcgttcacg tgcagttcga cgacaaggac gtccgctgcg agttcaagac
     3181 cggagtcgac acctgcaagg acgagggcgt ctccttggac ggcgacatcg ggcacgtgta
     3241 cgggttcaga cttgctgggc gacacccgga cacggtcacc ttctcgtttt ccgatctgaa
     3301 ccaggttctc gtctcggcga cggtgaaccc gaagttcgtc acgcagcagc ccaacgggcc
     3361 caactgcacc cccacgtgcg acaacggttt cgtccagctc tgaccaacgg cggggtcgtg
     3421 cgctcccttt gcattgcggc ggtacccgct tcgccgaccg cgtcatggac aatccccccc
     3481 tctgcgccat cctgaccgga ccaaaccgaa aaggggcgtg aatgatcacc cggtcaaact
     3541 gacgctcaag caaaccggtc tttgagccgt tgctgagggg tttacgtgat agcgccatct
     3601 cgtcgggtcg ccgccactcc cacatcatgg cggcgagatg tttcgcgtgc ccatctgtcc
     3661 ttgtcataca ccgaccatcc aattcgacgg caatatcacc ggacaca
//
LOCUS       DAOSOO010000275         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25090_length_3690_cov_4.355708, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000275 DAOSOO010000000
VERSION     DAOSOO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25090_length_3690_cov_4.355708"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLT86_13710"
     CDS             <1..883
                     /locus_tag="PLT86_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37713.1"
                     /translation="DLHGETSSLDKDGRRHVRWCDGESGHWNWGEKFKTAEDVFAFSP
                     LEQGDFTSIPVVESQDYSSEEKLYERYSRGYPKEWGNRGREGSSSMVGFYNTMFMWPL
                     LTFGWELFLETCLDPRFERIMDEFAEINRRVFRTFARLPVNFVLCHDDIVTSRGPVCS
                     PKWMHKYIFPRYEEFWGIVKAAGKEVIFMADGCMDAFADDVMACGARGIISEPYTDYK
                     RIARNYDNPFLAGEGDNRVLTRNDPSEIEAMVRSMVETSRMTGGYVMCIGNHIPWNVT
                     PEGIKRYLDLSAELAVR"
     gene            890..1300
                     /locus_tag="PLT86_13715"
     CDS             890..1300
                     /locus_tag="PLT86_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37714.1"
                     /translation="MGKTTFASITAWFHGSPLRLTTLRKGSTITPDRDLARVFSHRPS
                     LVSIKTGPHGFIRHSGTDPGFLYFVEVGTKADVIPHPTTTMEPGDEWITTRDLPLSLV
                     EPTVVREEERLSDDEVAELKARAEAQRSETRPPK"
     gene            complement(1622..2752)
                     /gene="cfa"
                     /locus_tag="PLT86_13720"
     CDS             complement(1622..2752)
                     /gene="cfa"
                     /locus_tag="PLT86_13720"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HOT37715.1"
                     /translation="MVSDTVRELLDHAGIHINGEEAWDIRVHDNRFFGRVLAEKNLGL
                     GESYMDGWWDCPRIDEMICRLLRAGVEDRVRGNLRYALPFIRAYLFNLQKVSRARIIA
                     ERHYDTGNDLFFSFLDPYNQYSCAYFEGTDDLNRAQRQKMALIAGKLGLSKTDTLLDI
                     GCGWGGLARYAAETTGCRVTAVNISREQIGFAREFCKGLPVEVVECDYRSIHGKFDKI
                     VSVGMFEHVGRKNYRTFMEVAWRSLKDDGVFLLHTIGSLRSKSGTDPWIEKYIFPNSM
                     LPSIAQISKAAEGLFVIEDLHNLGPHYDKTLMAWHSNFQRNWPQLAEKYGERFKRMWE
                     YYLLSCAGAFRARGIQIWQIVMTKHGTGTAQPQCRFVPALPE"
     gene            complement(2886..>3690)
                     /locus_tag="PLT86_13725"
     CDS             complement(2886..>3690)
                     /locus_tag="PLT86_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37716.1"
                     /translation="PRIHSIDSRVGGLGFLWLLGCVPAMGVVLFRGKQYVALKYRGPM
                     WGLLIAVFVSLIATPANWWARYTLWIYVAGLPCLVIVVSRWLQQGRLMRDLAVVWCVS
                     AMLPFSLEVGWSLCKLAQMAEMEALVFGGDSFHGLRERTARVTPAYPRSVFSADMRGT
                     EFEALDTTRAVAIGPLGGGVLRGNALKFEIAGALSRPIGFRELFYLPAIPSESNMNRL
                     KMNRVRYVIWDDGKAIPALLATEYDSVCSRNGFLLLKRKFDLPDDARRG"
BASE COUNT          779 a         1198 c          996 g          717 t
ORIGIN      
        1 cgacctgcac ggagagacct cctcgctcga caaggatggc cgccgccacg tgcgctggtg
       61 cgatggcgag tcgggacact ggaactgggg cgagaaattc aaaacggccg aggatgtgtt
      121 cgcgttttcc ccccttgagc agggcgactt caccagcatc ccggtcgtcg aatcgcagga
      181 ctactccagc gaggagaagc tctacgagcg ctacagccgg ggatatccca aagaatgggg
      241 aaacagaggg cgtgaaggct cctccagcat ggtcgggttc tacaacacca tgtttatgtg
      301 gccactcctc acgttcgggt gggagctttt cctcgaaacg tgcctcgatc cgcgctttga
      361 gcgcatcatg gacgaattcg ccgaaatcaa ccgccgcgtg ttccgcacgt tcgcccggct
      421 gccggtgaac ttcgtcctgt gccacgacga catcgtcacg tcgcgcggcc cggtctgctc
      481 gccgaaatgg atgcacaaat acatcttccc gcgctacgag gagttctggg gcatcgtcaa
      541 agcggcgggt aaggaagtca tcttcatggc agacggctgc atggacgcct ttgccgacga
      601 cgtgatggcg tgcggcgcgc gcggcatcat cagcgagcct tacaccgact acaagcgcat
      661 cgcccgcaac tacgacaacc ccttcctcgc cggcgaaggc gacaaccgcg tgctcacccg
      721 caacgatccc tcggaaatcg aagccatggt gcgctccatg gtcgaaacca gccgcatgac
      781 gggcgggtac gtcatgtgca tcggcaacca cattccctgg aacgtcacgc cggaaggcat
      841 caagcgctat ctggatctat ccgccgaact ggcggtgagg taaggattgg tgggcaaaac
      901 gactttcgcc tccatcaccg cgtggttcca tggttccccg ttgcgcctta cgacactccg
      961 caaaggcagc acaatcactc cggatcgcga cctggcgcgg gtgttctcgc acagaccttc
     1021 gctcgtctcg atcaagaccg gaccccacgg tttcatcagg cacagcggca ctgatccggg
     1081 ctttctctac ttcgtggaag ttgggaccaa ggccgacgtc attccgcacc ctacgacaac
     1141 catggagccg ggcgacgagt ggatcaccac gcgcgacctg cccttgagcc tggttgagcc
     1201 cacggtggtt cgggaggaag agcggctgag cgacgacgaa gtggccgagt tgaaggcacg
     1261 cgccgaagcc caaaggagcg aaacgcgccc tccgaagtga atggaatgcg gtgctctttc
     1321 gcgattcccg gagtacagac ctgtcattcc cgcgtgctcc accgagcacg atgcgcaagc
     1381 gggaatccag cctttgtcgc cgcctgtttc ccgctccgcc ctattttctg cggttgcccg
     1441 cggtttgccg tcattcccgc gaaggcggga atccagttct gccattaccc cggaaggcag
     1501 gaaccccgat cgggggcgtg ccccccgagg cccctgacgc acacctctct gtcgccattc
     1561 cgagcacgag catctgtgcg cgcggcaaaa aaaatctccg ttgaggcccg tcatcccttt
     1621 gtcattccgg caacgccggc acgaaccggc actgcggctg tgccgttccg gttccgtgtt
     1681 tggtcatcac gatctgccag atctggattc ctcgtgcacg aaacgcgccg gcgcacgaca
     1741 acaggtaata ctcccacatc cgtttgaacc gctcgccgta tttctccgcc agttgcggcc
     1801 agtttctctg gaagttgctg tgccaggcca tgagcgtttt gtcgtaatgc ggcccgaggt
     1861 tgtgaagatc ctcgataaca aaaagcccct ccgccgcctt tgatatttgc gcaatgctcg
     1921 gcagcatgct gttcgggaag atgtacttct cgatccaggg gtcagtaccc gatttcgatc
     1981 tgaggctgcc gatggtgtgc agaaggaata ccccgtcgtc tttcagagag cgccacgcca
     2041 cttccatgaa cgtccggtaa ttcttccggc ccacatgctc gaacatcccc acggaaacga
     2101 ttttgtcgaa tttcccatga atgctccggt aatcgcactc cacaacctct accggcaggc
     2161 ccttgcagaa ctcgcgggca aaaccgatct gctcccgcga aatgttcacc gcagtcaccc
     2221 tgcatcccgt cgtctccgcg gcgtaccgcg ccaggccacc ccatccgcac ccgatatcaa
     2281 gcagcgtgtc ggtctttgag agccccagct ttcccgcaat cagcgccatc ttctgccgtt
     2341 gcgcccgatt caggtcgtcg gttccctcga aatacgcgca gctgtactgg ttgtacgggt
     2401 cgaggaagga gaaaaacagg tcgttaccgg tgtcgtagtg gcgttcggcg ataattcgag
     2461 cgcgggagac cttctgcaga ttgaacagat acgcccggat gaagggcaag gcgtacctga
     2521 ggttgccccg taccctgtcc tcaacaccgg cccggaggag ccggcagatc atctcatcga
     2581 tccgggggca atcccaccat ccgtccatgt aggattcgcc cagcccgagg ttcttttcgg
     2641 ccagcactcg accgaaaaac cggttgtcgt gaaccctgat gtcccatgcc tcttcaccgt
     2701 tgatatggat acccgcgtga tccagaagct cccgcacagt atcggatacc atcgctccag
     2761 tcctccctga ttggctcagg ccgaccggta aggcactttg cccggtttcc tgctacccct
     2821 cctgttccat gatattcgcc gattgttcga cgtaacgaat tcgaaggctg cacgaagttg
     2881 ccattctacc cccgcctcgc atcgtccgga agatcgaact tgcgtttcag caggaggaat
     2941 ccattcctgc tgcataccga atcatattcg gtagctagaa gggccgggat cgctttcccg
     3001 tcgtcccaaa tcacgtaccg aacccggttc attttcaagc ggttcatgtt actctcggat
     3061 ggaatggccg gcaagtagaa aagctctcgg aaaccgattg gccgcgagag cgcgccggca
     3121 atctcgaact tcaacgcatt gcctcgaagc accccccctc ccagcgggcc aatggcgacc
     3181 gctctcgtgg tatcgagcgc ctcaaattct gttccccgca tatcggcact gaaaacagag
     3241 cgcgggtacg ctggtgtcac gcgcgcagtt cgctctctca acccgtgaaa actatcgccc
     3301 ccaaacacga gagcctccat ttcagccatt tgcgccaact tacagagaga ccaaccgact
     3361 tccagggaga agggaagcat cgcagacaca caccacacaa ccgcaagatc gcgcatcagg
     3421 cgcccctgct ggagccacct cgacacaaca atcaccagac agggaagccc ggcgacatat
     3481 atccataacg tgtatctcgc ccaccagttg gcaggagtgg caatgagact gacgaaaacg
     3541 gcaatgagca gtccccacat ggggcccctg tatttcagag ccacgtattg cttgcctctg
     3601 aacaggacga cgcccatcgc cggaacacaa ccgagcagcc aaaggaaacc caggccgcca
     3661 accctcgaat cgatactgtg aatcctcggc
//
LOCUS       DAOSOO010000276         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25138_length_3685_cov_4.633609, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000276 DAOSOO010000000
VERSION     DAOSOO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25138_length_3685_cov_4.633609"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="PLT86_13730"
     CDS             <1..1463
                     /locus_tag="PLT86_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37717.1"
                     /translation="DVVNDRLQPLYNFLATPPELKRQPVPRVKRSYMKRFAVSNLGTI
                     DTSRGCPFACTFCTIINVQGRKMRCREASDLRNTIIENYNQGINFYFFTDDNFARNKN
                     WESILDLIIDLNNEGRHITFMMQIDVRAYKIPGFVEKSKRAGCSQVFVGMESINPDNL
                     KAAGKTQNDVGDYAHMVEVWHDAGVAVHVGYIIGFPFDTPESVADDVRRLADEMKVDQ
                     ASFFMLTPLPGSVDHKRMVENGEEMDSDFNRFDSFHAATNHPRMTKAQWFETYQRAWR
                     DFYRFDRMRAIMRRTPRRHYWNVFTNFMWYKNSFLSEDAHPMVTGFIRRKKRCERRPG
                     MPREERWSYFLNRLREIKKELVTRWSLSRELQELWLQTRRRTEVENRVADALHELALQ
                     RKRIRFRIEDWKAAYSAAQAQVPSAWKIVVAKVNVLSLRWTYTRQDLDNFWRQTRRQW
                     ECRAFHRINWPLATWYAFRDAVLSLRFALHMALRLQ"
     gene            1763..2206
                     /locus_tag="PLT86_13735"
     CDS             1763..2206
                     /locus_tag="PLT86_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT37718.1"
                     /translation="MLAETARQNPYAQSADVIAAVPLHRVRERERAFNQSDLLAAAVA
                     AALGKPLASTALVRTRATPRQATIQSRAERMANVRGAFHVKDPRAICGKTVLLVDDVI
                     TTGATVCALAETVLEAGALAVSAAAVAHPFPSGPMETYDPQIWTV"
     gene            complement(2258..2333)
                     /locus_tag="PLT86_13740"
     tRNA            complement(2258..2333)
                     /locus_tag="PLT86_13740"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2297..2299),aa:Glu,seq:ctc)
     gene            2507..3421
                     /locus_tag="PLT86_13745"
     CDS             2507..3421
                     /locus_tag="PLT86_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37719.1"
                     /translation="MNFSAVSFAYLVDSEETEEQAAVLSRSIRDLGGGLCASQIIAMR
                     PETSRPSRRWNALKDDLQVIESVFAMEVELSTLPFATKAVAAGHAESRVAESGDLLVW
                     MDSGSLVLREPAELLLDDDHVLGCSPVDHTLIGSPFSEPLDGFWEYVYRVCGVDRNSV
                     FPVTSVVDRREIRAYFNAGLLVVKPQRGILRAWGENVRRICTGADLKGLLNGNPLHRV
                     FLHQAVLAATILARVDRNEILLFTPHVNYPLHMHTDHPANQATKSMDDLVTCRYEGFF
                     ARDDWAETAPVSDALEAWIRTNLCRADS"
BASE COUNT          793 a         1046 c         1096 g          750 t
ORIGIN      
        1 cagacgtggt caacgatcgc ctgcagccat tgtacaactt tcttgctacg ccgccggagc
       61 tcaaacgaca accggttccg cgagtgaaac ggtcctacat gaaacgcttc gccgtttcaa
      121 atcttggtac tatagacacc agccgcggct gcccgtttgc ctgcacgttc tgcaccatta
      181 tcaacgtcca ggggcggaaa atgcgatgca gagaggcgag cgaccttcgc aacaccatca
      241 ttgaaaacta taaccagggc ataaactttt acttcttcac cgacgacaat tttgcccgga
      301 acaagaactg ggaatccatc ctcgacctga tcatcgatct gaacaacgag ggtcgtcaca
      361 taaccttcat gatgcagatt gatgtgcggg cgtacaagat tcccgggttt gtggagaaat
      421 cgaagagggc gggatgctct caggtgtttg tcggtatgga aagtatcaac ccggacaacc
      481 tgaaagcagc tggaaagacg caaaacgacg tgggtgatta cgctcacatg gttgaggtct
      541 ggcacgatgc gggagtggcg gttcacgtcg gctacattat cggcttcccg ttcgataccc
      601 cggagtcggt cgcggatgat gtccgccgcc tggcggacga aatgaaggtg gaccaggctt
      661 cctttttcat gctcacacct cttccgggtt ccgttgacca caagcgtatg gtggaaaacg
      721 gggaagagat ggattctgat ttcaaccgtt tcgattcatt ccacgccgca acgaaccacc
      781 cccgcatgac aaaagcgcaa tggttcgaaa cataccagcg agcctggcga gatttttacc
      841 ggttcgaccg tatgcgggcc atcatgcgaa ggacgccacg gcggcattac tggaacgtgt
      901 tcaccaattt catgtggtac aaaaactctt tcctttccga agatgcgcat cccatggtaa
      961 ccggattcat ccgcaggaag aagcgttgcg aacggagacc ggggatgccg cgcgaagaac
     1021 gctggagtta cttcctgaac cgtctccgtg aaatcaagaa agagcttgtg acccggtgga
     1081 gcctttcgcg cgagctccag gagctctggc ttcagacgcg ccggcgcacg gaagtggaaa
     1141 atcgcgtggc tgatgcactg cacgaacttg ctttgcagag aaagcgaatc cgtttccgaa
     1201 tcgaagactg gaaagcggca tacagcgcgg cgcaggcgca ggttccttcg gcatggaaaa
     1261 tcgttgtggc gaaggtgaac gttctctcgc tccgatggac ctacacgagg caggatctgg
     1321 acaacttctg gcgtcagacc cgaagacaat gggaatgccg ggcattccat cggattaatt
     1381 ggccgcttgc aacgtggtac gcgttccggg acgccgtgct cagtctgcgc ttcgccctcc
     1441 atatggcact ccggctgcaa tgagaatgct cgcgcggcgc gtgtcagaag cgttccttga
     1501 cgccatctgg ccgcccgcgt gtccggtgtg ccaaagacct ctggaaaccg ctcagcgatt
     1561 gtgcgcatcc tgcacgaccc aactggcacc tttgcaggcc cgctgcgagc gttgttccta
     1621 cccgatcccg tgccactcgt gcgcaaatct tcccacacct ctgacagcgt tgatcgcagc
     1681 cacggaatac gtgcgggcaa gggaactcgt ctttgcctgt aagtatggga gtaatcgatc
     1741 tgtcgggcgt tttcttgcag acatgctggc agaaacggcc cgacagaacc cctacgcgca
     1801 gagcgccgat gtgatcgccg ccgttccgtt gcacagggtt cgtgaacgag aacgcgcgtt
     1861 caatcaatcc gatttgctcg cggcggcagt ggcggccgca ctcggaaagc ccttggcaag
     1921 caccgccctc gtgcgtacga gagctactcc ccgccaggca accattcaat cccgcgctga
     1981 acgcatggct aatgtccggg gtgcatttca cgtgaaggat ccacgagcta tttgcggaaa
     2041 aacggttctt ctcgtggacg atgtcatcac caccggtgcg acggtctgcg cactggcaga
     2101 aactgtgttg gaggccggtg ctctggcagt cagtgcggcg gcggtcgcac atcccttccc
     2161 atccgggccg atggagacgt acgatccgca aatttggacg gtgtgagacc cactgtcttg
     2221 taggtgacga gttcgggggc gaaggggatt cgagcaatgg tagcgcgtac gggagtcgaa
     2281 cccgtgtttc cggcgtgaga ggccagcgtc ctgggccact agacgaacgc gccaaagcgg
     2341 aagaatatta aagttaacgc caaacccggc gtttgccaaa agccgcgggc cccggcccac
     2401 gcgaattgcg attgaaccag aactggtcgt ctccgttttt caggcgtacc gatcggtacc
     2461 gacaggcggc tgagcctgcg tcagccacag gaactcaaca ctgcaaatga acttctccgc
     2521 agtatccttc gcatatctgg tggattccga ggagacggaa gaacaagcgg cagtgctctc
     2581 acgcagcatt cgcgatctcg gcgggggcct ttgtgcgagc cagataatcg cgatgaggcc
     2641 ggagacatcg cgtccttccc gcaggtggaa cgctcttaaa gacgatttgc aggtaatcga
     2701 gagcgtgttc gcgatggaag tggagttgtc caccctgcca ttcgccacga aggcggtcgc
     2761 ggcgggacat gcggaatcgc gggtggcaga aagtggcgat ctactcgtgt ggatggacag
     2821 tggctcgctt gtcctgagag agcctgcgga gttgttgctg gacgatgacc acgtgctggg
     2881 ttgctcgccg gtcgaccaca cgctgatcgg gtcgccgttt tcagagcccc tggatgggtt
     2941 ctgggagtac gtgtaccggg tttgcggggt ggaccgcaat tcggtgttcc cggttacaag
     3001 cgttgtggac cggcgtgaga tccgcgccta cttcaacgcg ggacttcttg tggtcaaacc
     3061 gcagcgcgga atccttcgtg cgtggggaga aaacgtgcga aggatttgca cgggtgcgga
     3121 cctgaaaggg ttgctcaacg gtaacccgct gcaccgggtc tttctacacc aggcggtact
     3181 tgccgcgacg attcttgcgc gagtggatcg gaacgagatc ctgttgttca ctcctcatgt
     3241 gaactaccct ctccacatgc acacggacca tccggccaat caagcaacga aatccatgga
     3301 cgatcttgtt acgtgccgct acgaagggtt cttcgcacgt gacgactggg ccgagactgc
     3361 cccggtgagc gacgcgttgg aggcctggat cagaacgaat ctgtgtcggg ccgattcctg
     3421 acgccacccg ggagtcatcg ggaggggttg cagccgcaca gacatacaca gagcatccgc
     3481 tttcatctca cgtaccgacc tcttgcgcga acgctctcac tggggaaaaa tcgggacgta
     3541 cgattgcgcc ttcctcgtca ggcatgctct ctcgccttcg gaaagaggcg cggtgttgtc
     3601 cgcgacccgt attgccaacc ggagcatttc ggcatcgccc ggcggaatgc acgcggtgac
     3661 gccctcagac caggcgaaac gcagc
//
LOCUS       DAOSOO010000277         3651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25434_length_3651_cov_5.859566, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000277 DAOSOO010000000
VERSION     DAOSOO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_3651_cov_5.859566"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PLT86_13750"
     CDS             complement(<1..1347)
                     /locus_tag="PLT86_13750"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT37720.1"
                     /translation="MPTLHGRITDAANGELREAKVSVVDCEGGVRTPEGAVLKVGNGL
                     VSFYCGGEFSVPVKGGPCRIVVERGTEYEPLVKHLNVPWKGDLDVTLELRRWVDLPRM
                     GWYPGNTHIHYDQNEKQPYERLRLEPHVNDFAVTVVSILQRWDLPYASNRFPLGAFTE
                     FSTAHHVVDVGEEVRHNFWEGGFGYGHLLVFRIHDQVEPLSRGMLVNDFNPDYPPLCL
                     ACDDAHRQGGIVVWCHNGLGMEAPVAAALGKLDALNLFDPYWMNPEYDLWYKMLNCGF
                     HLPASTGSDWFVCSNNRVYACTGTDFSYDTWLAALKKGQTFITNGPALLLSVDGYGPG
                     AFLPCTSKRALEVKVEWRSFHPIEVVEIVRDGFVIARKEFATGSREGSFVQTVLPETD
                     GWIAARCSGNARDSFDQAIFAHTSPVYVQTGSPSPAREEAARFFADAIRGSVDWVNT"
     gene            1529..2014
                     /locus_tag="PLT86_13755"
     CDS             1529..2014
                     /locus_tag="PLT86_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT37721.1"
                     /translation="MARHKTILVVDDNPSMRAILAIWLNRFVHLPFRLHVLTAPDGEE
                     AWRILGASRVDLLLANHHMPKTPGTQLIRRVKSNTRLRNVPCVLESIGELPDLAAEGL
                     SCGADFVISLLNNDKSDLESVLAAALGVSSQTETDPGRTPHDSVLTPQWWGTSWNSSP
                     L"
     gene            1993..3360
                     /locus_tag="PLT86_13760"
     CDS             1993..3360
                     /locus_tag="PLT86_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT37722.1"
                     /translation="MEQLPLVISPPLSSSHEFAAAADVIRTLHAAGYDAYLIGGCVRD
                     MVLGLIPKDFDIVTSAKPEETQALFPRSLRLGAQFGVITVLANGLSFDVATFRREGNY
                     EDGRRPSWVEYADLEADVRRRDFTINGMAFDLSRSSVIDLCGGLTDLREGIIRTIGDP
                     AERFGEDALRVIRAVRFAARFGFSLEETTRLALMSAAPSLGRIARERIWQELEFMMTA
                     EHREVAFRLLAELGLLPHVFGPDVVRSSETIVRTSARLEALASDASIVLVLACVLADG
                     VARAAPNKSADRLVEPYDEEAASRAEAVLASLRAPRSVVDGVGALIARRMVWAAQNSL
                     REGRIAQLVRNDRTGALVSFWAADAASFGLADAVSRIRDIADRIIAEGKAHGGKSSMP
                     VNGTVLAEAGVPRDATMGELLAEAERLWLEGKLETEADVRRWLADAVRSAGASPCPGN
                     SKPQA"
BASE COUNT          690 a         1101 c         1129 g          731 t
ORIGIN      
        1 cgtgttgacc cagtcaactg agccacggat cgcatctgca aagaaccggg cggcttcttc
       61 ccgtgccggg gaaggagagc ctgtctggac gtagaccggg ctcgtatgcg cgaagatggc
      121 ctgatcgaag ctgtcgcggg cattgcctga gcagcgggcg gcaatccacc cgtcggtttc
      181 cgggagcact gtctgaacaa acgatccctc ccggctcccg gttgcgaact ccttgcgcgc
      241 aatcacgaaa ccgtcgcgga ctatctcgac aacctcaatg gggtggaaag aacgccactc
      301 gactttcacc tcgagggcac gttttgaggt gcagggcaga aacgcacccg gcccgtaacc
      361 atccacggaa agaagcaacg ccgggccgtt cgtaatgaat gtctgaccct tcttcagcgc
      421 cgccaaccat gtgtcgtatg aaaagtctgt cccggtgcag gcgtatacgc ggttatttga
      481 gcagacgaac cagtcgcttc cggtgctcgc cggcagatgg aatccgcagt tgagcatttt
      541 gtaccacaag tcgtactccg ggttcatcca gtagggatca aaaaggttca gcgcgtccag
      601 cttgcccagc gcggctgcca cgggggcttc catgccgagg ccgttgtggc accacaccac
      661 tattccgccc tgccggtgcg cgtcgtcgca cgccaagcag agcgggggat agtcggggtt
      721 gaaatcgttg accagcatcc cccggctcaa cggctccacc tggtcgtgga tacggaacac
      781 cagcagatgg ccgtatccga accctccctc ccagaagtta tgccggacct cttctcccac
      841 atccaccaca tgatgtgccg tgctgaactc ggtgaacgcg ccgaggggaa aacggttgga
      901 tgcgtacggg agatcccacc gctggagaat gcttacgacc gtgacggcga agtcattcac
      961 gtgtggttca aggcgaagcc gttcatacgg ttgtttttcg ttctgatcgt agtggatgtg
     1021 cgtgttgccc gggtaccagc ccatgcgggg gagatcaacc catcgccgca gctccagagt
     1081 gacatcgagg tcgcctttcc aggggacgtt gaggtgtttc accagcggct cgtactcagt
     1141 accgcgttcc acaacaatgc gacaggggcc acccttcacc ggaacggaga actccccgcc
     1201 gcagtagaac gaaaccagcc cgttccccac cttgagaacc gccccctccg gtgtgcgtac
     1261 tcccccctcg caatcgacca cgctgacttt cgcttcgcgg agttcgccat tcgccgcgtc
     1321 agtgatgcgc ccgtgcagtg tcggcattgt ccacctccct gcggtgatga agaagccttt
     1381 cgaaatagta tcgttattgc cccgccccgg acaaaatgag atgggaggca cgaagcggcc
     1441 ttgccagatt tcacaaaccg catatctttg tgaactggag cacactccca ccaatgacag
     1501 atcgtcccga acgccgcggg tgcatgctgt ggccagacac aaaaccatcc ttgtcgtcga
     1561 cgacaacccg tccatgcggg cgattctggc catctggctg aaccggttcg ttcatcttcc
     1621 cttccgcctt cacgtgctta cggcaccgga tggcgaggaa gcgtggcgaa tactcggcgc
     1681 ttcccgcgtg gaccttctgc ttgccaatca tcatatgcca aaaacacccg gcacccagtt
     1741 gatccgccgg gtgaagtcga atacgcggct gcggaatgtc ccgtgtgtac tggaaagcat
     1801 cggggaactc ccggacctcg ccgccgaggg actgtcctgc ggtgcagatt ttgttatttc
     1861 cctcctgaac aacgacaaat ctgatctcga gtcggtactt gcggcggcgc ttggagtttc
     1921 gtcgcagacg gaaaccgatc cggggcgaac tccgcacgat tctgttctga cgccgcaatg
     1981 gtggggcacc tcgtggaaca gctccccctt gtgatatccc cgcccctctc ctcgtcccat
     2041 gagtttgccg ccgccgctga cgtaatccgc acccttcatg cggcggggta cgacgcgtac
     2101 ctgatcggcg gctgcgtgcg tgatatggtg ctcgggctga ttccgaagga tttcgacatt
     2161 gtcacgtcgg cgaaaccgga ggaaacgcag gcgctctttc cgcgatcgtt gcggctcggc
     2221 gcacaattcg gcgtcatcac cgtgctggca aacggtctct cgtttgatgt ggccaccttc
     2281 cgcagggaag gcaactacga agacggtcga cgaccttcgt gggtggaata cgccgatctt
     2341 gaagcggacg tccgcaggcg cgatttcacg atcaacggga tggcgtttga cctgtccagg
     2401 agttccgtga tcgatctttg cggggggctc acggacctca gggaaggcat catcagaaca
     2461 atcggggacc cggcggagcg attcggggag gatgcgttgc gcgtcatccg cgccgtgaga
     2521 tttgcggcga gattcgggtt ttcgctggaa gaaaccaccc ggctcgccct catgtcggcc
     2581 gctccatccc ttggcagaat cgcgcgggag cggatctggc aggaactcga attcatgatg
     2641 acggcggaac accgcgaagt ggcattccgt cttcttgccg aactcgggct tctaccacac
     2701 gttttcgggc ccgatgtcgt ccggtcgtcg gaaacgattg tccgcacttc ggcgcgcctt
     2761 gaggcgctgg cctcggatgc atcgattgtg ctggttctcg cgtgcgtgct ggcagacggc
     2821 gtagcacgcg cggcgccgaa taaatccgcc gacaggcttg tcgagccgta cgacgaagag
     2881 gcagcctcac gcgccgaggc cgtgctggcg tcacttcgcg cgccgcggtc ggtggtggat
     2941 ggggtcggcg cgctgattgc ccggcgcatg gtctgggcgg cgcagaactc tctcagagag
     3001 gggcgcatcg cacaactggt gcgcaatgac agaaccggcg ccctcgtgtc gttctgggcg
     3061 gcagatgcgg cgtcgttcgg gttggcggac gccgtttccc gaattcgtga catcgccgac
     3121 cggattatcg ccgaagggaa agcacacggc gggaagagca gcatgcccgt gaacggcacc
     3181 gtgcttgcgg aggcgggagt gccgcgcgac gcgaccatgg gtgagcttct cgcggaggca
     3241 gaacggctct ggctggaagg gaaactggaa acagaagctg atgtccgacg ctggctggcc
     3301 gacgccgttc gatctgcggg cgcgagcccg tgcccgggaa actcgaagcc acaggcgtga
     3361 gttgtcccat ttctgggatt gaagcgctat attggagatg ggcaatcttg atctcggggg
     3421 gatcacgtgg gaagcataag ggcatgcggt cggtctactg ggatttactc catatcgttg
     3481 cgttcgtgtt gtttctcctc ttcgtggcag gcgttgcagc aaatgttgcc gggtacggcc
     3541 cccgggaatg gtttctgcct ctaatgattg ccgctgcgat gtccgcggcg gctgtgctgg
     3601 caacggatca aagcagacgc tggccctggt tccggcgcgg ttcacagtga a
//
LOCUS       DAOSOO010000278         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25476_length_3646_cov_6.086048, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000278 DAOSOO010000000
VERSION     DAOSOO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25476_length_3646_cov_6.086048"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="PLT86_13765"
     CDS             complement(<1..1904)
                     /locus_tag="PLT86_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT37723.1"
                     /translation="MVLLQLDKISHSYGANEILHRITWQIKDDTRAGLIGPNGCGKTT
                     LARIITGEIAPSSGTITQSGNVSIGYLRQESAAPPGHTVWSYIAGADARLADLRTRLA
                     ALEETIARDYQSPADSSRGDVPPDLIAAYGAAHDEYEHMGGYEYDARCASVLSRMGFR
                     AEDYDQPVELLSGGQQSRLTLAALLVRQPDVLVLDEPTNHLDIEATEWLEGFLSSYKG
                     AVIVISHDRVFLDRVVTEIVEIERMRLVHYRGNYSAYAAQKEERRQQETKAYELQREM
                     IERTEEFIRRNIAGQKTKQAQGRRKQLEKLERLTPPPFQRSVSLSFVASHRGGNEVIE
                     VRSLSKAYNGLPLFEELSFLVRRGERVGVVGPNGSGKSTLLRMLVGEEEPDGGIIRFG
                     VGVEAGYYDQRREGLDQESRVIDEVWSVKPSLTTEQIRSYLGSFLFTEEEQFRIIGTL
                     SGGEQSRVALAKLVLAQVNLLILDEPTNHLDIPARLALENALDRYDGTLLVVSHDRAF
                     LRRLTTRVIRIQDGAAQVFDMGYRALEAAQAEAKEKEAREAVARGSATEQAVPRQVVA
                     ETSPADKAARLAAYERKRELERRRRQRERRFADIEAEIMDVENRLMEIDDELGNPAHA
                     ADWEMLRTLTA"
     gene            2038..3237
                     /locus_tag="PLT86_13770"
     CDS             2038..3237
                     /locus_tag="PLT86_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37724.1"
                     /translation="MRFRAARWRALPTAIWALGFGSLFMDISSELIHSLLPVFMVSSL
                     GASMVVVGVVEGVAEATAAFVKLFSGALSDWFGRRKPLLVLGYALGAASKPVFPLAGS
                     VAWVFAARFVDRVGKGIRGAPRDALVAEVAPANMRGAAYGLRQALDSVGSLAGPLLAV
                     PLMVWFLGDIRSVLWVATFPAVVSVLVLIAYVREPERVEVRGAAGPRLMFAGVGRLPG
                     RYWIVVLLGAVFTLARFSEAFLVLRARDTGMPDGRVPLVLVVMNLFYAAGAYPAGSAA
                     DRINRRVLLLGGLALLVASNLVLAGATTPPTVLFGVALWGLHMALTQGLLAKLVSDAT
                     PGELLGTAFGVFHLVSGAMLLVASIVAGALWTTQGASATFLAGAAFAAIAAIGVWSVA
                     RRSAPEP"
     gene            3336..>3646
                     /locus_tag="PLT86_13775"
     CDS             3336..>3646
                     /locus_tag="PLT86_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37725.1"
                     /translation="MKHGTVAAAYLVAAMLVSALQVHAQEPPDQQARPARMRDAVVIG
                     EQQATIRLMPLKDPNLAAILALGTPGMGHFYVGNWKRGAAFLTGVMGSVVAVGLAGDN
                     LA"
BASE COUNT          607 a         1106 c         1166 g          767 t
ORIGIN      
        1 gcggtgaggg tgcgaagcat ctcccagtca gcggcgtggg caggattgcc gagttcatcg
       61 tcgatttcca tcaggcggtt ttccacatcc atgatctccg cctcgatgtc ggcgaaacgg
      121 cgttcacgtt gccttcgcct gcgctccagt tcgcgctttc gttcgtaggc ggcaagtcgg
      181 gccgccttgt ccgcgggaga agtctccgcg acaacctgtc tcggaaccgc ctgttcggtt
      241 gccgaaccac gagcaacggc ttcccgcgcc tccttctcct ttgcctcggc ctgagccgcc
      301 tcaagcgcgc gatagcccat atcaaacacc tgtgcggcac cgtcttgaat ccggatcact
      361 cgcgtcgtaa gccgtctgag gaacgcgcgg tcgtgcgaaa ccacaagcaa tgtcccatcg
      421 tagcggtcga gcgcattctc cagcgcaagg cgcgcgggaa tatcgaggtg gttggtaggt
      481 tcgtccagga tcagcaggtt gacctgcgcc agaacgagtt tcgccagcgc gacccgactc
      541 tgctcccctc ccgaaagcgt gccgatgatg cggaactgct cctcctcggt gaacaggaat
      601 gaaccaaggt acgaccggat ctgttctgtc gtgaggctcg gttttacgct ccagacctcg
      661 tcaatgacac ggctttcctg atcaaggcct tcgcggcgct ggtcatagta tcccgcctcc
      721 acgccgacgc cgaagcgtat gataccaccg tccggctctt cctcaccgac cagcatgcgc
      781 aggagggtgg acttgccgga cccgttcggc ccgaccacgc caacccgctc gcccctgcgc
      841 acaagaaacg aaagctcttc gaacagcgga agaccattgt acgccttgct cagcgaacgg
      901 acctcgatca cctcatttcc accgcgatgg ctcgccacaa acgaaaggct caccgaccgc
      961 tggaacggcg gcggggtgag acgctccagt ttctccagtt gcttccgtcg cccctgcgcc
     1021 tgtttggttt tctgtccggc aatgttgcgg cggatgaatt cttccgtgcg ctcgatcatc
     1081 tcgcgctgca attcgtaggc tttcgtctcc tgttgccgcc tctcctcttt ttgagcggcg
     1141 tatgcgctgt agttgccccg gtagtggacg agccgcatgc gttcgatttc gacgatttcc
     1201 gtaacgacac gatcgaggaa cacccggtca tgggaaatca cgatcaccgc gcctttgtag
     1261 gacgacagaa atccttccag ccactctgtt gcttcgatgt cgaggtggtt tgtgggctca
     1321 tccagcacga gtacgtcggg ttggcgcacg aggagcgccg caagggtgag ccggctctgc
     1381 tggccgccgc tcagcagctc cacaggctgg tcataatcct ccgcgcggaa gcccatgcgc
     1441 gagagaaccg acgcacagcg ggcgtcgtac tcgtatccgc ccatgtgctc gtattcgtcg
     1501 tgcgccgcgc cgtaggcggc aatgaggtcc ggaggcacgt cgccccggga tgaatcggca
     1561 ggcgactggt aatcccgcgc gatggtttcc tcgagggcgg cgaggcgagt acgcaggtcc
     1621 gcaagacggg cgtctgcccc ggcgatatag ctccagacgg tatggcccgg cggcgcggct
     1681 gattcctgcc tgagatagcc gatggacacg ttacccgatt gcgtaatcgt gccgctcgaa
     1741 ggtgcgatct cgccggtgat gatacgtgca agcgtagttt tgccgcaccc gttagggcca
     1801 atcagaccgg cgcgtgtatc atctttgatc tgccacgtga tgcggtggag gatttcattg
     1861 gcgccgtacg agtgggagat tttgtcaagc tggagaagaa ccattccgga gtgtacctgt
     1921 gcgagggcaa aagagtaccg aacgacctgc tgtacagaaa atacgaatga gtgacccggg
     1981 aaacgcgcgt tgtttcggtt ccgtgtatca aagggccggg aggttcaggt ggcgaacatg
     2041 agattccgag cggcccgctg gcgcgctctt cccacagcga tctgggcgct cgggttcggc
     2101 tcgttgttca tggacatctc gtccgaactc atccacagcc ttcttccggt gttcatggta
     2161 agttcgctgg gcgcatccat ggttgtggtg ggtgtcgtcg aaggcgtagc cgaggcgacc
     2221 gccgccttcg tgaagctctt ttccggtgct ctgagcgatt ggttcgggcg tcgcaaacct
     2281 ctgctggttc tcgggtacgc actcggagcc gcaagcaaac cggtatttcc gcttgcgggc
     2341 tccgttgcat gggtgtttgc cgctcgtttt gttgacagag tcgggaaagg catcaggggc
     2401 gcgcccaggg atgcgctggt cgcggaggtg gctcccgcga acatgcgtgg cgcggcgtac
     2461 gggctccgtc aggcgctcga ttcggtcggc tcgctggcag ggcccttgct cgcggtaccg
     2521 ctgatggtct ggttcctcgg cgacatccgt tccgtgctgt gggtggccac cttcccggcg
     2581 gtcgtatccg tgctggtgct catcgcgtat gtgcgtgagc ccgaacgcgt ggaggtacgc
     2641 ggcgcagccg ggccgcgcct gatgttcgcc ggcgtggggc gtttgcccgg gcggtactgg
     2701 attgtcgttc tgctgggtgc cgtattcacg ctggcccgct tcagcgaggc attccttgtg
     2761 ctgcgggcgc gggataccgg gatgccggac ggacgggttc cactggtgct ggttgtcatg
     2821 aacctgttct atgcagcagg tgcatacccg gcagggtcag cggcagaccg catcaatcgc
     2881 cgggttcttc ttctcggcgg gctggcgctg ctggttgctt ccaacctcgt gctggccggc
     2941 gcaacaaccc ctccaacggt gctgttcggc gtcgcgctgt ggggtcttca tatggcgttg
     3001 acgcagggat tgctcgcaaa actcgtttca gacgcaactc cgggcgaact ccttggcacg
     3061 gcttttggtg tgtttcatct ggtatcaggg gcaatgctgc ttgtggcgag cattgtcgcc
     3121 ggcgcgctgt ggacgacgca aggcgcgtcc gccacgtttc ttgccggtgc agcgtttgcc
     3181 gcaatcgcag ccatcggggt gtggagcgtc gcacgccgtt ccgcgccgga gccctgaccg
     3241 gttcagaatg gtgcgaagat gtccgccgtc cggttcgcgg agagtgaaaa agacagtatg
     3301 tgaagttgcg ggagcacctc gcaaggaggg agaagatgaa acacggaacc gtcgccgcgg
     3361 cctacctcgt agccgcgatg cttgtgagtg cgttacaggt gcacgcgcag gaaccacccg
     3421 atcaacaggc ccgacccgcg cgtatgcgtg atgcggtggt gatcggcgaa cagcaggcca
     3481 ccatccgcct catgccgctc aaagacccga accttgccgc gattctcgcg ctgggaacac
     3541 cgggcatggg gcatttctac gtcggcaact ggaaacgggg agccgcattc ctcaccggtg
     3601 tcatgggatc cgtcgttgcg gtgggtctgg ccggcgataa cctggc
//
LOCUS       DAOSOO010000279         3643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25513_length_3643_cov_3.753902, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000279 DAOSOO010000000
VERSION     DAOSOO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25513_length_3643_cov_3.753902"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLT86_13780"
     CDS             <1..356
                     /locus_tag="PLT86_13780"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOT37726.1"
                     /translation="TRAGVDFNQPLVVVGTGVHSGRLPNNASFLSVDEPNIVLAAVKR
                     SEDGGAIIVRVYETDGCRTDAHVRFGTFLAVSEVNAVDLLERTTTDRVERDGNTIRVE
                     VPPFGISSLRVSFAK"
     gene            373..1224
                     /locus_tag="PLT86_13785"
     CDS             373..1224
                     /locus_tag="PLT86_13785"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOT37727.1"
                     /translation="MKLRNAIAASAAIGLVALVSGCGVLNKAVQKPKVSFEAVSVRSL
                     SSESVTMDVHLNVQNPNVFDVPLKKYSYALNIGESRLLSGEQGLSVTVPARGSGQISV
                     PLTVRFREVMGAIEAVTDTDVVPYSIGGTVTIDAGPLAGLTIPFNHSSSIPRPALPSI
                     TVERLSVTSLSLSGVNANLVLSVRNPGRLAYALENFAGDLELNGSKLATIDGIGGTQR
                     LPGGGAQTITVPLRLSSTQALTGIQSLLNSGAISYRIKGTAGVSADVLGRMPLTIDQS
                     GTTRITR"
     gene            1301..1744
                     /locus_tag="PLT86_13790"
     CDS             1301..1744
                     /locus_tag="PLT86_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37728.1"
                     /translation="MRKSVAGVLAGVVAVFGYAVGRPTFSSLGLLPNLGNVPGTATEC
                     QVCHVSAGGGGALNAFGGTVQANLTGGVPDWSRIRVLDSDGDGYSNGQELGDPLGYWQ
                     PGQPNPGLQTKITNPANAMSKPTGPSGVQIVTTPTAWGVIKALFR"
     gene            1812..2942
                     /locus_tag="PLT86_13795"
     CDS             1812..2942
                     /locus_tag="PLT86_13795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT37729.1"
                     /translation="MCRRLISSEKVPAELWNDWRWHIRNRITTLDELRCVVAPDEDET
                     ERLRRVVARFPLSITPYYASLIDFDDRQCPIRRQVVPSVEEIEDPDGVEDPLGEVEQS
                     PAQNVIQIYPDRVALTVVTQCPVFCRFCFRKRLFSTTRGGAVRGLEKTIEYIRGTPQI
                     KDVLVTGGDPLIASDSFLERLLRSLRSIPHVEIIRIGTRAPVVLPQRITPRLCAILSR
                     YHPLWVNTHFNHPKELTPEAREACDRLLRAGIPLGNQSVLLKGVNDDPETMRELVRGL
                     VRMRVRPYYLFQCHLVRGTRHFRTTIEQGLRITGALRGHVSGIANPLFVVDTPDGKVP
                     LLPRRGLKRREGDYVVLETFKGTEWREYNPTADGQRKGREDH"
     gene            2939..3286
                     /locus_tag="PLT86_13800"
     CDS             2939..3286
                     /locus_tag="PLT86_13800"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT37730.1"
                     /translation="MSNDAPLGDRARALARNLHVLTVMFPDSEKPLLGMQLRNAAIEL
                     VTEAVVASATDDPAEKQEMLRLAAVNCIRIRELIQLARELDHLPEDDVAELIAAVEVI
                     NATIARERRTARY"
     gene            complement(3293..>3643)
                     /locus_tag="PLT86_13805"
     CDS             complement(3293..>3643)
                     /locus_tag="PLT86_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37731.1"
                     /translation="LPGALSGHCAVSWGDRYVVLIGGVFRQETCGQMLAMSGVKLLDK
                     RKALTGEYSDLVWVYDTVTDTFTLMQERSPHGLNDIHACVVGNTIFTVGGENVDPATS
                     NTTDYVMLGKITGN"
BASE COUNT          748 a          992 c         1137 g          766 t
ORIGIN      
        1 cgacgcgcgc cggggtggat ttcaaccagc cgctggtcgt cgtaggaact ggtgttcatt
       61 caggccgcct gccaaacaat gcgagtttcc tgtctgtcga cgaaccgaac atcgttctgg
      121 cggcggtcaa acgaagcgaa gatggtgggg cgataattgt tcgtgtctat gaaaccgatg
      181 gttgccgaac cgatgcgcac gtgaggtttg gcacgtttct cgcggtttcg gaagtgaatg
      241 ctgtggatct tctggagcga acgacgacag acagggtgga gagagacggg aacacgattc
      301 gggtcgaggt gcccccgttt ggcatttcct ctctgagagt atcgtttgcc aaatagggta
      361 gggagcactg ccatgaaact caggaatgcg attgccgctt ctgccgcgat cggacttgtg
      421 gcactggttt cggggtgcgg agttctcaac aaggctgtgc agaaaccgaa ggtgtcattc
      481 gaggcggtat cagtgcgttc gctgagctct gagtccgtca caatggatgt ccatctcaac
      541 gtccaaaacc cgaacgtgtt tgacgtgccg ctgaagaagt actcgtatgc gttgaacatc
      601 ggggagagcc ggttgctgag cggcgaacag gggctctcgg ttacggttcc ggcacggggg
      661 tcggggcaga tcagcgttcc tctgaccgtg cgtttcagag aagtgatggg agccattgag
      721 gccgtgacgg ataccgatgt ggtaccctat tcgatcggtg gaacggttac gatagacgcg
      781 ggaccgcttg cagggttgac gattccgttc aaccattctt cgtccattcc ccggccagct
      841 ctcccctcga tcaccgtaga gcggctctcc gttacgtcat tgtcgctaag cggcgtgaac
      901 gccaaccttg ttctctccgt aaggaacccc gggagactgg cgtacgcgct ggagaacttc
      961 gcaggtgatc tggaactgaa cggaagcaag ctcgccacga ttgatggaat cggcggaacc
     1021 cagcgcctgc cggggggcgg cgcgcagaca atcactgttc cgctcagact ttcgtccacg
     1081 caggcgctga cagggatcca atcgcttctc aattcgggcg ccatttcgta tcgtatcaag
     1141 ggaacggcgg gtgtgtccgc agatgtgctc gggaggatgc cgctgacgat cgaccagagt
     1201 ggtaccacga gaattacccg ttgatgtttt cctccgggct gttgccatct gaggatgaat
     1261 cgtgtacgtt acaagcatga cggttactcg ggagaacgcg atgcggaaat ccgttgcggg
     1321 tgtgcttgcc ggcgtagtgg ctgtattcgg ctatgcggtg ggccggccta cgttctcttc
     1381 tctggggctg ctgccgaacc tcggcaacgt tcccggcacg gcgacggagt gccaggtgtg
     1441 tcacgtatcc gcgggtgggg gcggcgcgct taatgccttc gggggcaccg ttcaggcgaa
     1501 tctcaccggt ggcgtaccgg attggtcgcg catacgagtt ctggactcgg atggtgacgg
     1561 gtattccaac gggcaggaac tgggtgatcc gctggggtac tggcaacccg gccagccgaa
     1621 tcccggcctt cagaccaaga tcacaaatcc ggcgaacgca atgagcaaac ccacggggcc
     1681 ttcgggagtc cagatcgtga cgaccccgac cgcatggggt gtgatcaaag cgttgttccg
     1741 ctagtctacg cagctttgcc ctccgacatc ctacctgaca tccagctttc ccacaggcaa
     1801 agaacgccgc catgtgccga cgtttgattt cctctgaaaa ggttcccgct gagttgtgga
     1861 acgactggcg gtggcacatt cggaaccgaa ttaccactct tgacgaactg cggtgcgtcg
     1921 tcgcacccga cgaggatgaa acagaacgac tgcggcgtgt ggtggcgcgg tttccgcttt
     1981 cgatcacgcc gtattatgcg agccttattg acttcgacga tcgacagtgt cccatacggc
     2041 gacaggtagt gccttctgta gaggaaatcg aagatccgga cggcgtcgag gaccctttgg
     2101 gagaggtaga acagtctccg gcccagaacg tcatccagat ctacccggac cgggtggctc
     2161 tcaccgtcgt cacgcaatgc ccggttttct gtcgcttctg tttcaggaaa cgtctgttct
     2221 cgacgacaag ggggggagcg gttcgggggt tggagaagac catcgagtac atccgcggga
     2281 cgccgcagat caaagacgtg cttgtcactg gcggagaccc tttgatcgcg tcagacagct
     2341 ttctcgaacg gctcttaaga tcgttgcgaa gcattccgca cgtggaaatc attcgaatcg
     2401 ggacacgtgc gccggttgtt cttcctcaaa gaattacccc gcggctgtgt gcgattctgt
     2461 cgcgttacca tccgctatgg gtaaacacgc atttcaacca tccgaaggag ctcacgccgg
     2521 aagcgcggga agcctgcgac aggcttttgc gcgccgggat cccgctggga aatcaatcgg
     2581 ttcttctgaa aggggtgaac gacgacccgg aaaccatgcg cgagctggtg cgcggccttg
     2641 tacggatgcg cgtccgcccg tattacctgt tccaatgcca tcttgtgcga gggacccgac
     2701 attttcgaac gaccatcgaa cagggtcttc ggataaccgg tgcattgcgg gggcatgtct
     2761 ccggtatcgc gaatccgttg tttgtcgtcg acacaccgga cggcaaagtt cccctcttgc
     2821 ccagacgcgg gttgaaacga cgcgagggcg attacgtcgt tctggagacg ttcaagggaa
     2881 cggaatggcg cgaatacaat ccaacggcag acgggcagag gaaaggaagg gaagaccatt
     2941 gagcaacgac gcaccactgg gtgaccgggc acgagcgttg gcgaggaacc tccatgttct
     3001 gaccgtcatg ttcccggatt cagagaaacc gttgctggga atgcagctgc gcaatgcggc
     3061 gatcgaactg gtgaccgagg cagtggtggc gagcgcaacg gatgatccgg ctgagaagca
     3121 ggaaatgctg cgccttgcgg cggtcaactg catccggatc agggaactga ttcaactggc
     3181 gcgggaactg gatcacctgc ctgaggatga tgttgcagag ctgattgccg cggtggaggt
     3241 catcaacgcg acgatcgccc gcgaacggag gacggcgcgt tattgaacgc gatcaattac
     3301 cggtgatttt tccaagcatg acgtaatcag ttgtgttgct ggtggcagga tcaacgtttt
     3361 ctccgccaac cgtgaaaatc gtatttccca cgacgcacgc gtgaatatcg ttgagtccgt
     3421 ggggagagcg ctcttgcatg agcgtgaacg tatccgtgac ggtgtcgtac acccagacaa
     3481 gatcgctgta ttccccggtc agcgccttcc gtttgtcgag cagctttact ccgctcatcg
     3541 ccagcatctg gccgcaggtt tcctgccgga acactccccc gatcagaacc acgtaccggt
     3601 ctccccatga gaccgcacaa tggccggaaa gggcgccggg aag
//
LOCUS       DAOSOO010000280         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_25998_length_3593_cov_5.816280, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000280 DAOSOO010000000
VERSION     DAOSOO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25998_length_3593_cov_5.816280"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLT86_13810"
     CDS             <1..264
                     /locus_tag="PLT86_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37732.1"
                     /translation="RVTSGSVHLFGVVHGEIRFTMDHWSRNVHLFGYPGHSRESAELG
                     LALMACGGVKVKPLISVTVGFDNYLDGIARLKTGEAAKMCVVP"
     gene            280..1626
                     /locus_tag="PLT86_13815"
     CDS             280..1626
                     /locus_tag="PLT86_13815"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOT37733.1"
                     /translation="MAGECQIPLSVEMLREELRTLREQGAAEDVLDRIERDVAALPDG
                     APHGDLEVLYRRLEAVEPGPELAKREPTDLREIRGLRPEGPRRMRLECSDEALLDRML
                     GAWLARAAGCTLGKPVEGWTRQDIRALLEHVGEYPLRRYFPPVGQEWTGRRYHGWQLD
                     CLRGNIRYMARDDDMDYTLLGLVIMEAHGPGFTTRNVADEWLNTLPYHRVYTAETVAY
                     RNLVLGIQPPGTATLRNPYREWIGAQIRADFWGYAAAGLPELAAEFAWRDAILSHTRN
                     GVYGEMWMAATIAAAFAVNDVEQAIRIGLSEVPAECRLAKALNETLAWCNTDGALETT
                     LDRIQEEFGHYHPVHTINNAALVVAGLMYGRASLGATICGAVTGGWDTDCNGASAGSV
                     FGAMHGANGLPYEWVGCLNDTLHSALSGVNQGKPHRFTDLAQRTLVRHKKVAARLE"
     gene            1629..2873
                     /locus_tag="PLT86_13820"
     CDS             1629..2873
                     /locus_tag="PLT86_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT37734.1"
                     /translation="MSRNTMYALLAMLGIAAGTLVWRQWSYKRATGTQQALTRILEQT
                     DENPGERIRLLDSIMAAHPDDATIRRVAVYQKLRAHVELRSDSATVVRAVNDFLSTDT
                     SGAAFNFASSVYADLAINAPEGLRYAHRALAEVREVKRPKEMSEAQWAQQRRMMVGEC
                     HHILGRLYALSGQSRPALAALRAAADSVPDSPVILLRMAEIHESMGQLDSALESYMQA
                     LRLSHADTSALAGFARLYPAIHGPAVDPATVLDTLVAHARAKRKADLLAQRISQPAAP
                     FDLSTLAGRQYRLSDFRGKTLVLYFWATWCGPCKRQLPAIQEAHIRLKAHKDIELLTV
                     SFDQHFEVVPPFLARKRYDFPVVPGDEGLYKSYLLEGLPTCAVVDSTGTIRFKRLGNS
                     DAGDFLEELGWQLEAVSGKAPW"
     gene            3094..>3593
                     /locus_tag="PLT86_13825"
     CDS             3094..>3593
                     /locus_tag="PLT86_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT37735.1"
                     /translation="MPGTGILLRRAQQDGRPAFDRLQRSMEPRLRRFITRLVGNGDRV
                     RDIVQDTFLALYIKLERIVSEEHLWPFVFRVARNLCYDELRRQNRFEIIAVSHGSEHS
                     DDSFIPMATGRRADDITHWVMAVSLVKNAVDQLPEAQRQTLILHFEENLTYEQTAEAM
                     GVDIGTV"
BASE COUNT          696 a         1119 c         1141 g          637 t
ORIGIN      
        1 cgcgtgacat ctggctcggt gcacctgttc ggcgtggtgc acggggaaat acgtttcacg
       61 atggatcact ggagccgcaa cgtgcacctc ttcggatacc ccggacattc gcgtgaatcg
      121 gccgaacttg ggctggcgct gatggcgtgc ggaggtgtga aggtgaagcc cttaatcagc
      181 gttacggtgg ggttcgacaa ctacctcgac ggaattgcca gactcaaaac cggcgaagcg
      241 gccaagatgt gcgtcgtgcc gtagcgggaa gggacaggaa tggcagggga atgccagata
      301 ccactcagcg tcgaaatgct gcgcgaggaa ctgaggacac tgcgcgagca gggcgctgcc
      361 gaagacgtgc tggaccggat tgagcgggac gttgccgcac tgcccgacgg tgcaccccac
      421 ggtgatcttg aggtgctcta ccggcgtctt gaggcggtgg aacccggccc ggagcttgca
      481 aaacgcgagc cgaccgatct ccgcgaaatc cgcggtctgc gccctgaggg gccgcgccgg
      541 atgcggctgg aatgctccga cgaagcgctt ctggatcgca tgctcggagc atggctggcg
      601 cgggcggccg gttgtaccct cggaaagccg gtggagggat ggacgaggca ggatatccgc
      661 gcgctccttg agcatgtcgg cgagtatccc ctccggcgct atttcccgcc ggtcgggcag
      721 gagtggacgg gcaggcggta ccacggatgg caactcgatt gcctgcgcgg caacatccgg
      781 tacatggcgc gcgatgacga catggactac accctgctcg gccttgtgat catggaggct
      841 cacggcccag ggttcaccac gcgcaacgtg gccgacgagt ggctgaacac gttgccgtac
      901 caccgtgtct acaccgcgga aaccgtggcg taccgcaatc tggtgctggg tatccaaccg
      961 cccggcactg ccaccctgcg caatccgtac cgcgaatgga tcggcgcgca gattcgtgcc
     1021 gatttctggg gctatgccgc cgctggacta cccgaactcg ccgcggaatt cgcatggcgg
     1081 gatgcaattc tcagccacac ccgcaacggc gtctatggcg agatgtggat ggccgccacc
     1141 atcgccgccg catttgcagt gaacgacgtg gagcaggcca tcagaatcgg tctgagcgaa
     1201 gtaccggccg aatgccgtct cgcgaaggcg ctgaacgaga cgctcgcgtg gtgcaacacc
     1261 gatggcgccc tcgaaacgac gctggatcgg attcaggagg agtttggcca ctaccatccg
     1321 gtgcacacca tcaacaacgc cgcgctggta gtcgcggggc tgatgtacgg gcgggcctcc
     1381 cttggcgcaa cgatctgcgg cgcagtaact ggcgggtggg acaccgactg caacggtgcc
     1441 tcggccggat ccgtgttcgg ggccatgcac ggtgcgaacg ggctgccgta cgagtgggta
     1501 ggatgcctga acgacacgct gcacagcgct ctttccggcg tcaaccaggg gaaaccacat
     1561 cgcttcaccg atctcgcaca gcgcacgctt gtgcgacaca aaaaagtcgc tgcacgcttg
     1621 gagtagtcat gagccggaac acgatgtacg cactgctcgc catgctggga attgcggcgg
     1681 gaacgctcgt ctggcgccag tggtcttaca aacgagcaac cggaacccag caggctctta
     1741 cacgcattct ggagcagacg gacgagaacc cgggggagcg catccgcctt ctcgacagca
     1801 tcatggcggc tcacccggac gacgcaacga tccgccgcgt cgccgtgtac cagaagctcc
     1861 gggcccacgt ggaactcagg agcgactccg cgacggtagt tcgcgccgtc aacgacttcc
     1921 tgagcaccga cacgtcaggc gcggcattca acttcgcgtc gtcggtatac gccgacctcg
     1981 cgatcaacgc cccggaaggg cttcggtacg cccaccgggc tcttgcggaa gtgcgcgagg
     2041 tcaaacgccc caaggagatg agcgaggcgc aatgggctca gcagcgccgg atgatggtgg
     2101 gcgagtgcca ccacatactc ggccgtctgt acgccttgag cgggcaatct cgtcccgcgc
     2161 tggcggcgct gcgggcggcg gcggatagtg tccccgattc gccggtaatt ctgttgcgca
     2221 tggcggaaat ccacgaaagc atgggccaac tcgattctgc tcttgagtcg tacatgcagg
     2281 cgctccgctt gagccatgct gatacgtcgg cgctcgcggg ctttgctcgc ctctatcccg
     2341 ccattcacgg gcctgcggtt gatccagcca cggtgctgga tactcttgtt gcacatgccc
     2401 gtgcgaaacg caaggcggac ctgctcgcgc aacgcatttc ccagcccgcc gcgccgttcg
     2461 acctttccac gctggcgggt cggcagtacc gcctcagcga cttccggggc aaaaccctgg
     2521 tgctctactt ctgggcgacc tggtgcgggc cctgtaagcg gcaactgcct gccattcagg
     2581 aggcgcacat acggctcaaa gcgcacaagg atatcgagct tctcaccgtc agtttcgacc
     2641 agcactttga ggtggtgcca ccgttcctgg cgcggaaacg gtacgatttc cccgtggtgc
     2701 cgggggatga gggtctgtac aaatcctatc tcctggaggg cctccccaca tgtgccgtgg
     2761 tggattccac cggcacgatc cggttcaagc gcctcgggaa cagcgacgca ggcgatttcc
     2821 tcgaggaact cggctggcag cttgaggcgg tgtccggaaa agcgccatgg tgacccgctg
     2881 acctcccccg aacgaggaaa cggccatccg gcccgcttcc tgcgccctag gccgcctgtc
     2941 cgggcaccac gctgacatca gtccacaggt cccatcggac gtgtacctgt caactgcgcc
     3001 ggtagtctac gagatccggg ccattgcccc actttgaaca attggccttc ctcgttcgga
     3061 taaccctaca gagccatgca ggaaaggcgt tgaatgccgg gcacgggaat actcctcaga
     3121 agggcacagc aggatggacg ccccgctttc gaccggctgc agaggagcat ggagccccgg
     3181 ctccgcaggt tcatcacccg tctcgtcggg aatggagacc gagtccggga catcgtgcag
     3241 gacacgtttt tggcgctgta catcaaactc gaaaggatcg tctccgaaga acacctgtgg
     3301 ccgtttgtct tccgcgtcgc acgaaacctc tgttacgacg aactgcggcg gcaaaatcgg
     3361 ttcgagatta tcgctgtcag ccatggttcg gagcattctg atgactcttt cattccgatg
     3421 gcaacagggc gccgcgcgga cgacataacg cactgggtca tggccgtctc tttggtgaaa
     3481 aatgccgtgg accaacttcc cgaagcgcaa cgccagacgc ttatcctgca ttttgaggaa
     3541 aacctcacgt acgagcagac tgcggaagcg atgggcgtgg acatcggcac ggt
//
LOCUS       DAOSOO010000281         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26062_length_3587_cov_4.817667, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000281 DAOSOO010000000
VERSION     DAOSOO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26062_length_3587_cov_4.817667"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLT86_13830"
     CDS             <1..655
                     /locus_tag="PLT86_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37736.1"
                     /translation="CCRDEAVDVACVHLRRDNNGDGVYEPSVDDTLRYVGSRSFVQDD
                     GTVSFDFAVPETIAAGTSQYWLVVYHLALSVGPLPGSTFRAMLRSADYVAATGKASRA
                     PVTVEAVFPIQGGWKTITQAGVDRFELGQNLPNPFNPTTVIPFTLDRSSAVDLTVYDV
                     QGRVVRRLISGAHLEAGDHRVTWDGQTENGSSAGAGVYFVRLSAGNETRVRRMTLIR"
     gene            complement(730..1968)
                     /locus_tag="PLT86_13835"
     CDS             complement(730..1968)
                     /locus_tag="PLT86_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT37737.1"
                     /translation="MAERAKYGAAAMAGITGKLPVPFPREMGPNCLKYLEEYVASGLT
                     ADITGRFEAAFARELGVKHCIATPGCTPALAMLASAFSFAPGDEIIVSPITDYGTLQG
                     LIRENYIPVFADTDPGCINISARGIEPKITDRTRAILVVHKTGLMCDMEGINTLAARY
                     GLIVYEDACQAVFSEWKGRFAGTFGLAAGFSFDSEKTMGSDVGGCVVTNDDELAERLR
                     FVGLSRGAAMKPHFGRLHVVPGYAYRMPNCTAAICLGQLEIIREQVVRIDRAARLLNK
                     LVARIPGILPLRIPDQQNVWSCWMYGIRIDPTQFRCTPEEFADQLSQAGIPGAGMGKY
                     YLMPAALTFLDENARNKVYPYSMPPASREYRYSEENCPHAKAFLDNFIRWSTLCAKYE
                     PEHCELAAAIIAEVAERNRI"
     gene            complement(1979..2719)
                     /locus_tag="PLT86_13840"
     CDS             complement(1979..2719)
                     /locus_tag="PLT86_13840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT37738.1"
                     /translation="MWKNGARWVYSITYDEGCAALLDHALPVHRRYGVPGHVAVLASQ
                     IGVPRNVPGSSYHGMMILCTDEIHSLRAEGWGVSCHSMTHASITPENAHYEVVKSRAV
                     LEEVLGIPVPLFCVPGSNDCYPASLAVAREAGYSGIMTIYDDINTRQSDLMRLCRVPL
                     HTEYPPPFFSAFDPFKRIQQAIEAGGWIIDYCHCPMPGKPIHPWKDCTVEQLEERFQA
                     IRRFGGDEVWLAEPGEVVEYLLSAKHTP"
     gene            complement(2782..>3587)
                     /locus_tag="PLT86_13845"
     CDS             complement(2782..>3587)
                     /locus_tag="PLT86_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT37739.1"
                     /translation="KFRLAVTSLLVTQIEESIPEAFREAANGGADLILLPEEPDIVMG
                     WKPGLYRLTDHPIYSRMASFAAEYGIGAVASVSAVAGNGFANTGFLIDRKGALAGVYR
                     KQHPAPGEEAIVTDQAGDDPFPVFDFDGVVLGIAICMDVHFPEMFCIYGLKCADIVCL
                     PTMYMDYTGDMLESIEKARAVDNQYYVALSRYIETPYLAGKSMGYAKVIAPDGRIIAS
                     TGHQAGVAVAEFDPKWRMPFWGEQYKEFRTMFHTTRKPEKYGKIVEPKK"
BASE COUNT          702 a         1090 c         1028 g          767 t
ORIGIN      
        1 ttgttgtcgc gacgaagctg tggacgtggc atgtgtgcac ctgcgccgcg acaacaacgg
       61 ggatggggtt tacgaaccct ccgttgacga cacacttcgt tatgtcggct cgcgtagttt
      121 cgtgcaagac gatggaaccg tttccttcga ttttgccgtc ccggaaacaa tcgctgcggg
      181 aacgtcgcag tattggctag tcgtgtacca cctcgcccta tccgtggggc cgttgcctgg
      241 gagcactttc cgcgcaatgt tgaggtcagc agactatgtg gcggctactg gtaaggcttc
      301 gagggcgcca gtgacagtgg aggcagtttt cccgattcaa ggtggctgga aaacaataac
      361 acaagcgggg gtggaccgct ttgaactcgg tcagaacctt ccaaacccct tcaatcccac
      421 gacggtgatt ccgttcacgc ttgaccgttc gtctgcggtg gacctcaccg tgtacgacgt
      481 tcaggggcgg gtggtgcgcc ggctcatttc cggggcgcat ttggaagccg gcgatcacag
      541 ggtaacgtgg gatggtcaga cagaaaacgg ttcgtccgca ggtgccggcg tgtacttcgt
      601 ccggttgagc gcggggaacg agacgcgtgt gcggcggatg acgttgattc ggtgaacgtc
      661 ccgcgactac ccctgcgtgg cctctcatgt gtgcaggaac gccttcactg cgcccgccct
      721 gccctcgtgc tagatccgat tcctctccgc gacttccgcg atgatcgccg ccgccagctc
      781 gcagtgctca ggctcgtatt tcgcacacaa agttgaccac cggatgaaat tgtcgaggaa
      841 tgccttcgcg tgagggcagt tctcctcact gtagcgatac tcccgactcg ccgggggcat
      901 cgagtaggga tagaccttgt tccgtgcatt ctcatccaga aacgtcagcg ccgccggcat
      961 caggtagtac tttcccatcc ccgcaccggg aattccggcc tgggaaagct ggtcggcaaa
     1021 ttcctccggg gtgcaacgga actgagtcgg gtcaatgcga attccgtaca tccagcacga
     1081 ccagacattc tgctggtcag gaatcctcag ggggagaatg ccggggatcc gcgccacgag
     1141 cttgttgaga agccgcgccg cgcggtcaat tcgaaccacc tgctcccgga tgatctccag
     1201 ttgtccgagg cagatcgccg cggtgcagtt gggcatgcgg taggcatacc ccggcaccac
     1261 gtggagcctc ccgaaatggg gcttcatggc cgcgccccgg ctcaggccga caaaccgcag
     1321 gcgttcagcc agttcgtcat cgttggtgac cacgcatccg ccaacatccg accccatcgt
     1381 tttttcggaa tcaaaggaaa aacccgccgc aaggccaaag gttcccgcga agcgcccctt
     1441 ccattcgctg aatactgcct gacacgcgtc ttcgtacacg atgagcccgt atcgcgccgc
     1501 gagggtgttg atgccttcca tgtcgcacat gagacccgtt ttgtgaacga cgaggatggc
     1561 gcgcgttcga tccgtgattt ttggttcgat accccgtgcg ctgatgttga tacaacccgg
     1621 atcagtatcg gcgaacacgg ggatgtaatt ctcgcggatc aagccttgga gcgtcccgta
     1681 atcagtaatc gggctcacaa tgatctcatc tcccggcgcg aaggagaacg cggacgcaag
     1741 cattgcaagt gcgggcgtgc aacccggggt tgcaatgcag tgcttcactc ccagctcacg
     1801 ggcgaatgcc gcttcgaaac ggccggtgat atccgcggta aggccgctcg caacgtattc
     1861 ctcgagatat ttgaggcaat tcggccccat ttcacggggg aatggcaccg gcagcttccc
     1921 cgtgatgccg gccattgctg cggcgccgta tttcgctcgt tcggccatca cattctcctc
     1981 atggtgtgtg tttcgcgctc aagagatact ccaccacttc gcccggctcg gccagccaga
     2041 cttcatcgcc tccaaaacgc cgtatcgcct ggaagcgctc ctccagttgt tctacggtgc
     2101 agtctttcca cgggtgaatc ggcttccccg gcatggggca gtggcagtag tcaataatcc
     2161 acccgccggc ctcgatggcc tgttgaatgc gcttgaacgg atcgaacgcg gaaaaaaacg
     2221 gcggcgggta ttccgtgtga agtggaactc tgcaaagccg catcaggtcg ctttgccgcg
     2281 tgttgatgtc atcgtaaata gtcatgatgc ccgaataacc cgcctcccgc gcaaccgcaa
     2341 gtgacgcggg gtagcagtcg ttgcttcccg gaacgcaaaa caacggcacc ggaattccca
     2401 gcacctcctc cagcaccgca cggctcttca ctacttcgta gtgagcgttc tcgggcgtga
     2461 tcgaggcatg ggtcatgctg tggcatgaca caccccatcc ttcggcacgc agtgaatgta
     2521 tctcgtctgt acagaggatc atcattccgt ggtaactgct tcccggcaca ttacgtggca
     2581 cgccgatctg gctcgcgaga acagccacat gccccggcac gccatatctt cggtggaccg
     2641 ggagcgcatg gtcgagcagt gccgcacatc cttcgtcata cgtgatggag tagacccacc
     2701 gtgcgccgtt tttccacatt ccgtcctccc gcgcgttcac gccgggcgtc aatgagtcaa
     2761 tacgcctgag tcgttccggg ttcacttctt cggctctacg attttgccgt acttctccgg
     2821 cttcctcgtg gtgtggaaca tggttcggaa ctccttgtac tgctcccccc agaacggcat
     2881 acgccatttc gggtcgaatt cggccacggc gacccccgcc tgatgccctg tggacgcgat
     2941 aatgcgccca tctggcgcga tcactttggc gtaccccatg cttttgcccg cgagataggg
     3001 cgtttcgatg taacgtgaga gagcgacgta gtactggtta tccactgcgc gtgctttctc
     3061 gatgctttcg agcatgtcgc ccgtgtaatc catgtacatc gtcggaaggc atacaatgtc
     3121 agcgcatttc aacccgtaaa tgcaaaacat ctccgggaag tgaacgtcca tgcagattgc
     3181 gatcccgaga accacgccat caaagtcaaa caccgggaaa ggatcgtctc ccgcttgatc
     3241 agtcacaatt gcttcttcgc cgggggcagg atgctgcttc cggtacacgc cggcaagagc
     3301 accttttctg tcaatcagga atccggtatt cgcaaagccg ttccctgcca cggctgagac
     3361 cgatgccacg gcaccgatcc cgtactccgc ggcaaaggag gccatccggg agtagatcgg
     3421 gtggtcggtg agccggtaaa ggccgggttt ccaacccatt acgatatctg gctcctccgg
     3481 gagcagaatg agatccgcgc ccccgttggc cgcttcgcgg aacgcctctg gaatggactc
     3541 ctcgatctga gtgacgagta acgaggttac ggcaagccgg aatttcg
//
LOCUS       DAOSOO010000282         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26169_length_3577_cov_2.919364, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000282 DAOSOO010000000
VERSION     DAOSOO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26169_length_3577_cov_2.919364"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLT86_13850"
     CDS             complement(<1..437)
                     /locus_tag="PLT86_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT37740.1"
                     /translation="MTLEELRALMAAHEVEHLEFKEAKSNFDTGKLADYCVAIANEKG
                     GKLILGVTDKHPRKIVGSRAYNDLRSAKDFLRSKFPFLRIAVEELQTPEGRVLVFEIP
                     SRPIGMPLENHGAYLARCGESLVPMPPDQLRRIFAETEADFSAT"
     gene            complement(434..>3577)
                     /locus_tag="PLT86_13855"
     CDS             complement(434..>3577)
                     /locus_tag="PLT86_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT37741.1"
                     /translation="LLIKELIARGDLQRCLIVCPGNLVEQWQDELYRRFNLPFEILTN
                     DQLEASHSGNWFSETNLAIARLDKLSRDENVQQKLKAPECQWDLVVCDEAHKMSASFF
                     GNEVTYTKRYRLGQLLSSITRHFLLMTATPHNGKQEDFHLFMKLLDSDRFEGRFREGV
                     HEQDVSDIMRRMVKENLLKMDGTPLFPERTAITVPYALSEPEAELYRQVTSYVREEFN
                     RAEALDNDKRKGNVGFALTILQRRLASSPEAIYRSLTRRRERLESKLIDLQTNPAAQF
                     GSSPPRDYDLEDIDDVPEDEVETIEREVLDQATAARTAVELKAEIETLKGLELIAADV
                     RRSGTDTKWRELASVLSGVLSPPGLTDRVSEPDRLPYGAGPIAPPEPSPREKFVIFTE
                     HRDTLNYLERRISTLLGQPSAIVTMHGGMGREERRKAQESFLYDPSVRILLATDAAGE
                     GINLQRAHLMVNYDLPWNPNRLEQRFGRIHRIGQTEVCYMWNLVANGTREGEVYSRLL
                     EKLARAREALGGQVFDVLGKLQFEGKPLRELLIEAIRYGEQPEVRARLTQRVESALDL
                     DQLRTLLQERALTRETMDTSRVFQIGEMMQRAEARRLQPHYVESFFLAAFARLGGTIR
                     PREPRRYEVTYVPASLRNPGRSGGVRAPVLAKYERVVFDKSMIAPPGGAPAAFLSPGH
                     PLLNSVIDVTLDENAHLLRRGTVLVDERDDGTSPRVVFCLEHEIQDASVTSSGNRRVI
                     SKRALYIELSENGQASDAQYAPYLDFRPLKPDEPGAELILKRPECGWISAELEEKARE
                     YAVLHVVPEHLSEVRSRKLELADKTEAAVRERLVKEIAYWDRRAQEIKLREQAGSDTF
                     RLNSQEAQRRADNLRARFEKRMGELTRERQISPLPPVVLGGFLVVPSGLIELMKGIKT
                     PKLQDEVDKQAVAAKAREIVLETERALGYEPIDRELDKVGYDIESRVPNTGRLRFIEV
                     KGRVADAATITVTKNEILCSLNQPENYILAIVEFRNDGTHRVRYLRTPFGAEPDFDVT
                     SVNYDLAGLLARSEEPS"
BASE COUNT          648 a         1124 c         1043 g          762 t
ORIGIN      
        1 gtggccgaga agtccgcctc cgtctccgcg aagatccgcc ggagttggtc tggcggcatg
       61 gggaccaacg actcgccaca ccgagcaagg taggcgccgt ggttctccaa aggcatcccg
      121 atcggacggg agggtatctc aaacacgaga acgcgccctt cgggagtctg aagctcctca
      181 acggcgatgc gaaggaaggg gaatttgctc cgcaggaaat ccttcgcgct cctcagatcg
      241 ttgtaggcgc ggctgccaac aattttccgt gggtgtttgt cggtgacgcc caagatgagc
      301 ttcccgccct tttcattggc gatggctacg cagtaatccg cgagcttccc cgtgtcgaag
      361 ttgctcttgg cttccttgaa ctccaggtgc tccacttcgt gcgccgccat cagagcgcgg
      421 agttcttcga gtgtcacgat ggctcctccg agcgtgccag aagccctgcc aggtcgtagt
      481 tcacgctcgt cacgtcgaaa tccggttccg ccccgaacgg cgtgcgcaga tagcgaaccc
      541 ggtgcgtgcc atcgttccgg aattccacaa tcgccagaat gtagttctcc ggttggttga
      601 gcgagcagag gatttcgttt tttgttacgg tgatcgtggc ggcgtctgcc acccggccct
      661 tcacttcgat gaaacgcaac ctgccggtgt tgggcacgcg gctctcgatg tcgtacccga
      721 ccttgtccaa ttcgcggtct atcggttcgt accccagggc gcgctccgtc tccagcacga
      781 tctcccgggc cttcgcagca acggcttgtt tgtcaacctc gtcctgcagc ttcggtgtct
      841 tgatcccctt catcagttcg atcaggcctg aaggcaccac aaggaacccg cccagcacca
      901 ccgggggaag aggggaaatc tggcgctccc gcgtcaattc ccccatacgc ttctcgaagc
      961 gtgcccgaag gttgtccgca cggcgttgcg cttcctgcga attgaggcgg aacgtgtcac
     1021 tgcccgcttg ttcacgcaac ttgatctcct gagcgcggcg gtcccagtag gcaatctctt
     1081 tgacaagccg ttctcttaca gccgcctcgg tcttgtccgc caactccagt ttgcgcgagc
     1141 ggacttcaga aaggtgctcg ggcaccacgt ggagaaccgc atattcccga gccttctctt
     1201 ccagctcagc gctgatccag ccgcactcag gacgcttcag gatcagttcc gcgcctggtt
     1261 cgtctggctt caacgggcgg aaatcgaggt aaggcgcata ctgggcgtcg ctggcttgcc
     1321 cgttctcgct cagttcgatg tacagggcac gcttggagat gacacggcgg tttcccgaac
     1381 tcgtgacgct cgcatcctga atctcgtgct caaggcaaaa caccacgcgg ggcgacgttc
     1441 cgtcgtcacg ttcatcgacc agaaccgtgc cccggcggag aagatgggcg ttttcatcca
     1501 gcgtgacatc gatgaccgag ttcagaagcg ggtggccggg gctcaggaat gcggcaggtg
     1561 cacctcccgg aggcgcgatc atggatttgt cgaatacgac gcgctcgtat ttggccagca
     1621 ccggggcgcg cacaccgccg ctcctgccgg ggttgcgcaa cgacgccggc acataagtca
     1681 cctcgtaccg ccgcggctcc cgcggacgaa tcgttccgcc aagtcgcgcg aacgcggcca
     1741 ggaagaacga ctcgacgtag tgcggctgga gtcgccgggc ttcggcccgc tgcatcattt
     1801 cgccgatctg gaatacgcgg cttgtgtcca tcgtctctcg cgtaagcgct ctctcctgaa
     1861 gcaacgtgcg gagctgatca aggtcgaggg cactctcaac tcgttgggtc agccgcgcgc
     1921 ggacctccgg ttgttcgccg taccgtattg cctcgatcaa cagctcgcgc aagggcttcc
     1981 cttcgaactg gagtttgccc agaacatcga aaacttggcc cccgagcgct tctctggcgc
     2041 gtgccagctt ttccagcagt cgagagtaaa cctcaccctc ccgagtcccg ttcgccacaa
     2101 ggttccacat atagcagact tcggtctgcc cgatgcggtg gatgcgtccg aagcgctgct
     2161 ccagccggtt cgggttccag ggcagatcgt agttgaccat gaggtgagcg cgttgcagat
     2221 tgatcccttc gcccgcggcg tctgtcgcca ggaggatgcg caccgacggg tcatatagaa
     2281 acgattcctg ggcctttctg cgctcctcgc ggcccatgcc gccgtgcatc gtaacgatgg
     2341 ccgaaggctg cccgagtaac gtgctgatac gccgttccag gtagttaagc gtatcccgat
     2401 gttccgtgaa tattacgaac ttctcccgcg gcgatggctc gggaggcgca atgggccccg
     2461 cgccgtaagg cagccggtcg ggctcggaca ctcggtccgt cagaccgggt ggcgaaagga
     2521 ccccgctgag gacactcgcc agctcccgcc actttgtgtc tgtgccgctt cttcggacgt
     2581 ccgcggcgat cagttcgaga cccttgaggg tttcaatttc ggctttcagt tctactgcgg
     2641 tgcgcgccgc agttgcctgg tcaaggacct cccgttcgat ggtctcgact tcgtcttcgg
     2701 gaacatcgtc gatgtcctca aggtcgtaat cccgcggcgg agaactcccg aactgcgcgg
     2761 ctggattcgt ctggaggtcg atgagtttgc tctccagacg ttctctccgc cgcgtcagcg
     2821 accggtagat cgcctccgga gatgaagcca accggcgctg gaggatagtg agtgcaaatc
     2881 cgacgtttcc tttccgtttg tcgttatcca acgcctccgc cctgttgaac tcctcccgga
     2941 cgtagctggt gacctgtcgg tacaactccg cttccggttc ggaaagagca tagggcaccg
     3001 taatggccgt ccgctccggg aacagcggcg taccatccat cttgagaagg ttctctttga
     3061 ccatccggcg catgatatcc gagacatcct gctcgtgaac accctcccgg aaacgcccct
     3121 caaacctgtc gctgtccaga agcttcatga acagatggaa gtcttcctgc ttaccgttgt
     3181 ggggcgtcgc agtcatgagg aggaaatgcc gcgtgatgga ggagagaagc tggcccaacc
     3241 ggtaccgctt cgtgtaggtg acctcattcc cgaaaaacga cgcggacatc ttgtgcgcct
     3301 cgtcgcacac aacgaggtcc cactggcatt ccggtgcctt cagtttctgc tgaacattct
     3361 cgtcgcgaga gagtttgtca agccgggcga tcgcgaggtt cgtctcagag aaccagttcc
     3421 ctgaatggga agcctccagc tggtcgtttg tcagaatctc gaacggcaag ttgaaacggc
     3481 ggtacaactc atcctgccac tgttccacga gattccccgg acacacgatg aggcatcgct
     3541 ggagatcgcc ccgggcaatc agttctttga tcagcag
//
LOCUS       DAOSOO010000283         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26171_length_3576_cov_6.725362, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000283 DAOSOO010000000
VERSION     DAOSOO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26171_length_3576_cov_6.725362"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLT86_13860"
     CDS             <1..1078
                     /locus_tag="PLT86_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37742.1"
                     /translation="AGGMEAYRRDDYLRLVDDMAEAGMNSLMICIKWLTTGYRSRLPF
                     LDQLPGNPVIESDNGLLRAAIAAAHDRGMRVWLGAVVSIFVVENYGGAPYETSDRLPL
                     GFPIVPFGLYDPDMPEFEERAVAIAEEMVEQFPEIDGLEIEMEGCGRESAHRIAPYER
                     WAAATGAKPFAEIGHPFNPRVFDVPEWRDYTTARRIEVLKAVEQAVRGKGFTGALATL
                     WENSAVQYAVGMEVNLRMFHRECPGWTGITYEHDKWNHRYACMDLNVATPKELGMKVY
                     NLPRGVMTWCDQWPMPIRIEENWRMDVEDIQRFKPDGVWWMGSGSSNDGAHASLSRLR
                     ELGYPDGRAARKALLNTIAPLLRR"
     gene            1108..2523
                     /locus_tag="PLT86_13865"
     CDS             1108..2523
                     /locus_tag="PLT86_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT37743.1"
                     /translation="MAEEMPTTLSEPGRDVPVLASYDVVVCGGGPAGCAAAVAAARNG
                     ARTLVLEKEGYLGGAPCTQLVCVILSTNGVDFQGIWHEYIARLQARKAVRPFSRPFPG
                     GHIRSVTDPEQVKFVWDELLAEAGVEMLHHTYGAVCAVQDGAAKGVFVETRAGRQAIL
                     AKCVVDATGDGIVAAQTGIPFEQGDGEQLWAMALTKMFRMGNVRIPANWPDEEALKRI
                     ERELTEAIERGEYTAPVIVEKKRFMGYVRNWSWAMPENRTEFLSLISRVLKVNPLDPF
                     DLTRAEREGREQARQAADALCRFVPGFEEAYLLDTSNQIGVRSSRRLRGLATVSDEDV
                     WNFNKYPDSIARASWDVDVWPADSYSKAAVPLGTDGYKWRQERMERGDYFDIPYGAIV
                     ADGVDNLFMAGRCISASHLAQASLRIQQTCMSTGQAAGTAAALCVRTGASPRALDPQR
                     VIRQLEQDRAAVKPALDVSQL"
     gene            2553..3344
                     /locus_tag="PLT86_13870"
     CDS             2553..3344
                     /locus_tag="PLT86_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT37744.1"
                     /translation="MNEHAVLTVLGSCSGTEPMPGRHHTSIAMEYGGRTYWFDAGENC
                     AHAGYTGGVDLFGSEAIFISHAHMDHVGGLPHLLWTFQKIARATEEGKRRLTGRNIEI
                     FLPEMRPWKGLMTFIADDAGEYRGPFTLTPRLFDDGVIFDRDGVRVTALHNDHLGAMD
                     PHTSFSFRVDVGAKAFVFSGDVKHVSELAPLLDGVELLLMETGHHAPEEVCAWVKESG
                     KQIGKLVFVHHGRRILYDYAGELEKAQHILGERVLIANDGTRLEL"
BASE COUNT          676 a         1148 c         1167 g          585 t
ORIGIN      
        1 tgccggcgga atggaagcgt acaggcggga tgactatctc cggcttgtgg atgacatggc
       61 cgaagccggc atgaactcgc tcatgatctg catcaaatgg ctgacgacgg ggtatcggtc
      121 gcgcctgccg tttctagacc agcttcccgg aaacccagtg atcgagtcgg ataatgggct
      181 tctgcgcgca gccatcgcgg cggcgcatga ccgcggcatg cgggtgtggc tgggcgcagt
      241 ggtgagcatt tttgtcgtgg aaaactacgg cggcgcgccg tatgaaacgt cggacaggct
      301 tcccctcggg tttcccatcg tgccattcgg cctgtacgac cccgacatgc cggaattcga
      361 ggagcgcgcc gttgccattg ccgaagagat ggtcgagcag ttccccgaaa ttgacggcct
      421 tgagatcgaa atggagggct gcgggcggga atcggcccac cgaattgccc cgtatgagcg
      481 ttgggcggcg gcgaccggcg ccaaaccgtt cgcggagatc gggcatccct tcaacccccg
      541 cgtgttcgac gtgcccgaat ggcgcgacta caccaccgca cggcgcattg aggttctgaa
      601 ggcagtagag caggcagtcc gggggaaggg attcacgggt gctctggcaa cgctgtggga
      661 gaactccgcc gtccagtacg cggtgggcat ggaggtgaat ctgcgcatgt tccataggga
      721 gtgcccgggg tggacaggaa ttacctacga acacgacaaa tggaaccacc ggtacgcctg
      781 catggacctg aacgtcgcca cgccgaagga actgggcatg aaggtgtaca acctgccccg
      841 cggtgtcatg acctggtgcg accagtggcc catgccgatc cgtatcgaag agaactggcg
      901 gatggacgtg gaggatattc agcgcttcaa gccggacggc gtctggtgga tgggatcggg
      961 ctcgagcaac gacggggcgc acgccagcct gtcccgcctg cgcgaactcg ggtaccccga
     1021 cggcagagca gcccgcaagg cgctgctgaa caccatcgcg ccgctcttgc gccgctgatc
     1081 gaatcgcacg ggaaggtgaa tccgacagtg gccgaagaga tgccgacaac gctgagcgaa
     1141 ccgggacgcg acgtgccggt gctggcctcg tatgacgtcg tcgtgtgcgg aggcgggccg
     1201 gccggctgcg ccgcggcggt tgccgctgcg cgcaatggcg caagaaccct tgtgctggaa
     1261 aaagaggggt acctcggcgg ggcaccctgc acgcagcttg tgtgcgtcat cctgagcacc
     1321 aacggggtgg atttccaggg catctggcac gaatacatcg cccggctcca ggcgcgcaaa
     1381 gccgtgcggc ccttctcgcg ccccttcccc ggggggcaca tccgcagtgt caccgacccc
     1441 gagcaggtga agttcgtctg ggacgagctg ctcgctgaag ccggtgtgga gatgctccac
     1501 cacacctacg gcgcagtctg cgcggtgcag gacggcgcgg cgaagggcgt gttcgtggaa
     1561 acacgggccg ggcgccaggc gatcctggca aaatgcgtcg tggatgccac cggggacggc
     1621 atcgtcgcgg cgcagaccgg catccctttc gagcagggcg acggcgagca actctgggcc
     1681 atggcgctga cgaagatgtt ccgcatgggc aatgtccgca tacccgccaa ctggccggac
     1741 gaagaggcgc tcaaacgcat cgaacgggag ttgaccgaag ccatcgagcg cggcgaatac
     1801 accgcgccgg tgatcgtgga aaagaagcga ttcatgggct acgtgcggaa ctggtcgtgg
     1861 gccatgccgg aaaatcgcac ggaattcctc tcgcttatca gccgcgtgct gaaggtgaac
     1921 cccctcgacc cgttcgacct cacccgcgcg gaacgcgaag gacgcgagca ggcccggcag
     1981 gctgcggacg ccctctgtcg cttcgtgccg gggttcgagg aagcgtatct gctcgatacc
     2041 tcgaaccaga tcggcgtgcg ctcctcacgg cgcctgcgcg gcctcgccac cgtgtcggac
     2101 gaagatgtct ggaacttcaa caaatacccg gacagcatcg cccgggcttc ctgggacgtc
     2161 gacgtctggc ccgccgacag ctacagcaaa gcggcggtcc cgctcggcac ggacggatac
     2221 aaatggcggc aggagcgcat ggagcgcggc gactacttcg acatccccta cggcgctatc
     2281 gtagccgacg gggtggataa ccttttcatg gccggccgct gcatctccgc cagccacctc
     2341 gcgcaggcaa gcctccgcat ccagcagacc tgcatgtcca ccgggcaggc ggccggcacc
     2401 gcggcggccc tctgcgtgcg caccggcgca tcacctcgcg cactcgaccc tcagcgcgtc
     2461 atccgccagc tggagcagga tagagcggcg gtgaaacccg cgctggacgt ttctcagctt
     2521 tgagcagact gctttccgat ggggatttca tcatgaacga acacgcagtg ctcacggtgc
     2581 tcggctcgtg cagcggcacg gaacccatgc cggggcggca tcatacctcc atcgccatgg
     2641 aatacggcgg gcggacctac tggttcgatg cgggcgaaaa ctgcgcccac gcggggtaca
     2701 ccggcggcgt agacctgttc ggctccgagg cgatcttcat ctcccacgcc cacatggacc
     2761 acgtgggtgg ccttccccac ctgctctgga cgttccagaa aatcgcccgc gcaacggaag
     2821 agggaaaacg acgcctcacc ggccgcaata tcgagatctt tctgcccgaa atgcgcccgt
     2881 ggaagggcct tatgaccttc atcgccgacg acgccggcga gtaccgcggg ccattcacgc
     2941 tgacaccccg cctgttcgac gacggagtga tcttcgatcg cgacggcgtg cgggtgaccg
     3001 ccctccacaa cgaccacctc ggcgccatgg acccgcacac gtcattctcg ttccgtgtgg
     3061 atgtgggagc aaaggcgttc gtgttctccg gcgacgtgaa acacgtgagc gaactcgcgc
     3121 ctctgctgga cggggtcgaa ctcctcctca tggaaaccgg acaccatgcg ccggaagagg
     3181 tgtgcgcatg ggtgaaggaa tcggggaagc agatcgggaa actcgtgttc gtgcaccacg
     3241 gcaggcggat tctttacgac tacgccggcg aactggaaaa ggcgcagcac attctgggtg
     3301 aacgcgtcct catcgccaac gacggtaccc ggctggagct gtagaagcgc agacgacgcg
     3361 ccgctctcca cttcgcaccg catggagggg cccgctttgt cgggcccttt ccagtccctc
     3421 accctcgatt cattggcctt cgaagcccgg caaaggcatc accttcaccg agcgccattt
     3481 gcccgccggc gcgcaggtat accgctgcga ccctcctgcg ccctccggga aaacgccgcc
     3541 tccgccctgt cgagcctttc gaggatgcgc gccttc
//
LOCUS       DAOSOO010000284         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26304_length_3563_cov_4.255986, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000284 DAOSOO010000000
VERSION     DAOSOO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26304_length_3563_cov_4.255986"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="zupT"
                     /locus_tag="PLT86_13875"
     CDS             <1..714
                     /gene="zupT"
                     /locus_tag="PLT86_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT37745.1"
                     /translation="GTGFGLAIVRDLVEAYGGYWGHWANAAAFFGGMLLIGIIDNVIP
                     SAENPHTSPSEDETAPLQERPVTVSSRRSNAGSHTDHKKLLRMGLFTAFAITIHNFPE
                     GLATFLAALNDPGLGIAIAVAVALHNIPEGVSVSVPIYYATGDRKKAFMYSLLSGVAE
                     PVGAAAGYVAILLLVSDGGAIPPHIMGILFGGVAGIMVYISVDELLPTSRAYGKGHDS
                     LLGMVGGMLVMALSLLLMK"
     gene            791..2032
                     /locus_tag="PLT86_13880"
     CDS             791..2032
                     /locus_tag="PLT86_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOT37746.1"
                     /translation="MTLKKHSPRLGALEILKYIGPGLLVTVGFIDPGNWASNLSAGAD
                     FGYALLWVVSLSTVMLIILQHNVAHLGIVTGFCLSEATSLHVKAPYSTLILSSVMVAS
                     ISTSLAEILGASIALNMIFAIPVPVAAALVTIFVVVMLFTNTYKAVEKGIVALVSIIG
                     LSFLYELSLTTVEWDQVFVSWVTPSFPDGSMLIIMSVLGAVVMPHNLFLHSEIIQTRQ
                     WNLEDGAVIRKQLRFEFLDTLFSMLVGWAINSAMIILAAASFFNAKIPVVDLTQAQKL
                     LAPLLGEHSAFVFAVALLLAGVSSTMTSGMAAGSIFAGMFREPYDVKDSHTRIGIVIS
                     LVCALLVILFISNPFQGLIISQMILSVQLPVTIFLQVYLTSSAKVMGEYRNGPLLIVT
                     LVVLGTIVSALNVLLLISFFR"
     gene            2287..3396
                     /locus_tag="PLT86_13885"
     CDS             2287..3396
                     /locus_tag="PLT86_13885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37747.1"
                     /translation="MRREYFVPETLTLWKVATPYPANGNLLYPAGIEHTVVHRAGRDK
                     YRFLHDCAIVAHNGVLFAAWYNCPEGEIVGESLIRGRRSADDGSTWSDVEVIAADGDG
                     KGVFYVPVQFLSTGDDLFAYVSTMTGHDLVVSCEAFRLGASGAFSDWVPCGTIAPLFL
                     PNSTPVTTDDGTFLMAGRCAERAGQLPMIPAVARSKAPLGDWEVVRLLPEGILPDGRS
                     LKYPETTLIVEGADVTAFVRNDHGFALVFLSTDGGRTWRGPFEQNMPLGAAKMCSGIL
                     STGQRYIVFNTPSDGYRDLLTIAVSRPGEREFSTVWKVAHGFSEVLGCGSEWSYPCAL
                     EHNGMLLIAYTSEKHHCVVARIPVASLIGFPPSRE"
BASE COUNT          676 a          979 c         1086 g          822 t
ORIGIN      
        1 ggcaccggct tcgggctggc gatcgtgcgc gatctggtcg aggcctatgg cggctactgg
       61 ggtcactggg ccaatgccgc ggcgtttttc ggcggcatgc tgctcatcgg gatcatcgac
      121 aatgtgatcc cgtcggcgga gaacccgcac acctcgccat ctgaagacga gacggcgccg
      181 ctgcaggaac gccctgtgac ggtatcgagc cggagaagca acgccggttc ccataccgac
      241 cacaaaaaac tccttcggat gggtcttttc accgctttcg ccatcactat tcacaacttc
      301 cctgaggggc tggcgacgtt cctggccgcc ctgaacgatc cggggctcgg tattgccatc
      361 gcggttgcag tggcgctgca caacatcccg gaaggagtca gcgtctccgt gcccatttac
      421 tacgcaaccg gagaccgaaa aaaggccttc atgtactccc tgttgagcgg agtagctgag
      481 ccggtgggcg cggcggcggg ctatgtggct attctgttgc tcgtgagtga tggcggcgcc
      541 atacctcctc atataatggg gatcctgttc ggcggggtgg ccggtatcat ggtgtacatc
      601 agcgtggacg agttacttcc cacgagccgc gcgtatggca aagggcacga cagccttctc
      661 ggaatggtcg ggggaatgct ggttatggcg cttagcctgt tactgatgaa atgataagcc
      721 gggcgaaaaa tcggcgcgtt ttcggttttt gtgctgcttt tcaaagtatt tccgagacac
      781 ggatatccac atgactttga agaaacactc gccgcgtctg ggtgcgctgg agatcctgaa
      841 gtacatcggt cccggtttgc tcgtcaccgt ggggttcatc gaccccggga actgggcgtc
      901 gaatctttcc gccggggcgg acttcgggta tgcattgctg tgggtggtct cgttatcaac
      961 ggtgatgctc ataatcctcc agcacaacgt cgcccatttg ggtatcgtta cggggttctg
     1021 tttgtctgaa gccactagct tgcacgtgaa ggcgccctac tccacgctga tcctgtcctc
     1081 cgtgatggtt gcgtcgatat ccacctccct ggcagaaatc cttggggcga gcatcgcact
     1141 gaacatgatc tttgccatcc ctgtcccggt tgctgcagcc ctggtaacga tatttgtcgt
     1201 cgtgatgctg ttcacgaaca cctataaggc tgttgaaaaa ggcattgttg cgctggtatc
     1261 gattatcggt ctttcgttcc tctacgaact gtcattgacc accgtggaat gggatcaggt
     1321 tttcgtgagc tgggtcacac cctcattccc agacggctca atgctgatca tcatgagtgt
     1381 gctgggtgcc gtcgtaatgc cgcacaatct gttcctgcac tcagagataa tccagactcg
     1441 tcagtggaac ctggaagatg gtgcggttat tcgcaagcag cttcggttcg aattccttga
     1501 cacgttgttt tcgatgctcg tcggttgggc gataaacagc gcgatgatca tcctcgcagc
     1561 cgcgtccttc ttcaatgcga agattcctgt cgtggacctt actcaggctc aaaagctgct
     1621 ggcgccgctc ctgggagaac attcagcgtt tgtgttcgcc gttgcattgt tgctggcagg
     1681 cgtctcttcc accatgacat cgggcatggc tgcgggaagc atttttgcgg ggatgttccg
     1741 ggagccttac gacgtcaagg actcgcacac aagaatcggg atagtcattt cgctggtgtg
     1801 cgcattgctc gtgatactgt tcatcagcaa tccgtttcaa gggttgataa tttctcagat
     1861 gattctgagc gtgcaacttc cggtgacaat cttcctgcag gtctatctta cctcatctgc
     1921 aaaggtgatg ggcgagtaca gaaacggccc tctgctcatt gtcacccttg ttgttctcgg
     1981 gacgatagtc tcggcgctga atgttctgct tctcatcagc tttttccgtt gaaccgctga
     2041 aaggctgacc ggactgtggc gttgctttcg ttaccgttcc gttccctctc tgcactcggc
     2101 gggaggcagg acgggcacgg tcccacggcc caaacgggaa aaatccgccg tttccacgag
     2161 ctggcgagtc accatcggga gcgttccaca tggattcccg cctgcgtggg aatgagggat
     2221 caacgcgggg gaggggcgtc attcttaggg aagcgaagaa actgacacgc cacgcaggaa
     2281 cacggcatga ggagggaata cttcgtgcct gagactctca ccttgtggaa ggtggcaacg
     2341 ccctacccgg cgaacggcaa ccttctttac cccgccggaa tcgagcacac tgtcgtgcac
     2401 cgcgcaggca gagacaaata cagatttctc cacgactgcg caatcgtcgc gcacaatggc
     2461 gtcctttttg ccgcatggta caactgcccc gaaggtgaga tcgtcggcga gtcactcatc
     2521 cgtggcaggc gatcggctga tgacgggagc acgtggtccg acgtggaggt tatcgcggcg
     2581 gacggagatg gcaaaggcgt attttacgtc cccgtgcaat tcctgtccac cggggatgac
     2641 ctgttcgcgt acgtttcgac gatgacgggt cacgacctgg tggtctcgtg cgaagcgttc
     2701 aggctcggtg cctcgggggc gttttcagac tgggttccct gcggcacaat cgcaccgctt
     2761 ttcctgccaa actcgacccc ggtaacgaca gacgacggaa cattcctcat ggcaggacgg
     2821 tgcgccgaac gggcgggcca gttgccgatg attcccgccg ttgcgcggag caaggcgccg
     2881 ctcggtgatt gggaggttgt aaggctcctg cccgaaggca tacttccgga tggtcggtcg
     2941 ttgaaatacc ccgaaacgac gttgatcgtg gaaggggcgg acgtgactgc cttcgtccgg
     3001 aatgaccacg gttttgcgct cgtgttcctg agcactgacg gcgggaggac gtggcgcggg
     3061 ccgtttgagc agaacatgcc ccttggcgcg gcgaaaatgt gttcgggcat actgagcacc
     3121 gggcagcggt acatcgtctt caacacgccc tcggacgggt atcgcgatct tctcacgatc
     3181 gcggtcagca ggccgggtga gcgcgagttt tccacggtct ggaaggtcgc ccacgggttt
     3241 tctgaagtgc tgggctgtgg ttccgagtgg tcgtacccct gcgcgttgga gcacaacggg
     3301 atgctgctca tcgcgtatac ctcagagaag caccattgtg tggtcgcgag gataccggtt
     3361 gcgtccttga ttggattccc gccttcgcgg gaatgacgcg ccgggggcaa atcccgcgga
     3421 gggagagcgc agaaatgatg tgacgggcca ccctcgagga cgatgttcgt tcccggcagg
     3481 aggcttacaa agggatcgtt ccggatgagc gtgttgagca tcggactgtc gaaaaacggt
     3541 ctgaaagctg gcgcagagtg ttt
//
LOCUS       DAOSOO010000285         3530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26621_length_3530_cov_5.063022, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000285 DAOSOO010000000
VERSION     DAOSOO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26621_length_3530_cov_5.063022"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLT86_13890"
     CDS             complement(<1..251)
                     /locus_tag="PLT86_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT37748.1"
                     /translation="MSFNAKLIAVLKQDRRFVDEDGELVLAAVQDHAWRIDHGLVKLL
                     LADKDIKAKFFDEIEGHWVFNFNTFIEYVSQKNFLDNSYT"
     gene            complement(307..1401)
                     /locus_tag="PLT86_13895"
     CDS             complement(307..1401)
                     /locus_tag="PLT86_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37749.1"
                     /translation="MSGRQFNVWVFGDAHVGNDIRFGRESLADALRHSERGGEKGGDP
                     FPWDIAVDIGDMCGSQGLPEDGEGREVVRQFAALREHPREAVYSVCGNHDRSGLREPA
                     AWWWQKWVDPIGEHTQFSGVDPARRPFPVSGTWERYSFRAGNLLFLMMSDINEPTQTI
                     GRGDLGGNPGGVVSGETFRWWREMVESNPDHIIISVHHYMLKDTTVASGEWEGMDRDE
                     NGNWRGGYHGYKRQGTPIGASYLYWVDSQPDAQAFEHYLAEHPGSVAIWLGGHTHAHP
                     DATDGGKTHIETKWGTHFVNCGALTRYHNNVRHQNPPKSRLFTFKEGSDEVRVRCYLH
                     TDEFLPQGWYGRAERRLRVGSGFVSGRSLT"
     gene            complement(1412..2173)
                     /locus_tag="PLT86_13900"
     CDS             complement(1412..2173)
                     /locus_tag="PLT86_13900"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT37750.1"
                     /translation="MTNALSPSVQMQFMRPAQLEAAARAFPVVYVPFGLIEWHGRHLP
                     LGNDALKAHGILVKTAEKFGGVVYPPVYFPHDVNVPADITPEQAAADWRLCMLSALFE
                     RLKQTGFRVIIGVSGHNVQSQIDMVNQALKPVVADGTITGVGLWEISLSQCEESNTDH
                     AAKWETSNMQFFYPDLVDMAALGTAPLAPGMKPPDGIGGLDPREHASAEVGRRNVELA
                     SEAIGLKAQELLASLPPEHRAFRLPGISPEHWWAI"
     gene            complement(2229..2813)
                     /locus_tag="PLT86_13905"
     CDS             complement(2229..2813)
                     /locus_tag="PLT86_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37751.1"
                     /translation="MGDLQETFHQWHHSPAHMFVPGSTYMVTAATYGKQPFFDTAAKK
                     DLLLAELFEQTKHYGWQLEAWAILNNHYHFVGKGGENAADLSRLIKRVHSETAVRINE
                     MDGTPGRKVWFQYWDSCLTHEKSYLARLHYVHQNPVKHRVVKDAEAYPWCSMGWFLRE
                     AEGGFRRTVMSFKCDSVNVLDDFQPLDCASSFAL"
     gene            2982..>3530
                     /locus_tag="PLT86_13910"
     CDS             2982..>3530
                     /locus_tag="PLT86_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37752.1"
                     /translation="MYRFPVEHVYLVDTVPSNPQAQRRALRLATALGVPSPEVISLER
                     SLELRHSRQASRPGTYPRRTGEFDYSRGWTVVLDTYCEGERFVDRVPRATYRDGGRQM
                     GGEGVCRSALELHGAFGCYHRCAYCFVDPYFLIACDLETLADRMPEYLARFPAQKLWK
                     FDALTDTIVLEPEYGASELLVPL"
BASE COUNT          597 a         1160 c         1059 g          714 t
ORIGIN      
        1 gtgtaggagt tgtccaggaa gttcttctgc gacacgtact cgatgaaggt attgaagttg
       61 aagacccagt ggccctcgat ctcgtcgaaa aacttggcct tgatgtcctt gtcggccagc
      121 agcagtttga cgaggccgtg gtcaattcgc caggcgtgat cctgcacggc cgcgagcact
      181 aattccccgt cctcgtcaac gaaacgccgg tcctgcttga gaaccgcgat cagcttggca
      241 ttgaatgaca ttggcttctc ctgtggctat cccgtcatcg cttcttgtgc acattggtta
      301 tatgattcat gttaacgatc gacctgatac aaaaccagac cccaccctca accgcctctc
      361 cgcccggccg taccagccct gcgggaggaa ctcgtccgtg tgcaggtagc accgcacgcg
      421 cacttcgtcg ctgccttcct tgaacgtgaa cagccggctt ttgggcggat tctggtgccg
      481 cacgttgttg tggtaccgcg tcaacgcgcc gcagttgaca aaatgcgtgc cccacttcgt
      541 ctcgatgtgc gttttgccgc cgtcggtggc gtccgggtgg gcgtgcgtgt gtccgccgag
      601 ccagatcgcg acggaaccgg ggtgttccgc cagatagtgc tcaaacgcct gcgcgtccgg
      661 ctggctgtcc acccagtaga ggtacgacgc gccaatcggc gtgccctgcc gcttgtatcc
      721 gtggtagccg ccgcgccagt tgccgttctc gtcacggtcc atgccctccc actcgcccga
      781 ggccacggtg gtgtccttga gcatgtagtg gtgcacactg atgatgatgt ggtccgggtt
      841 cgattccacc atctcgcgcc accagcggaa ggtctccccg ctcaccacgc cgccggggtt
      901 gccgccgagg tcgccgcggc cgatggtctg cgtcggctcg ttgatgtcgc tcatcatgag
      961 gaagagcaga ttgccggcgc ggaaggagta gcgctcccac gtgccggata ccgggaaggg
     1021 ccgccgggcc gggtccaccc cggaaaactg cgtgtgctcc ccgatcgggt ccacccactt
     1081 ctgccaccac caggcggccg gttctctcag gccgctccgg tcgtggttgc cgcagacgga
     1141 gtacacggct tcgcgtggat gctccctgag cgcggcgaat tggcggacca cctccctgcc
     1201 ttccccgtct tccggaaggc cttgggaacc gcacatgtcg ccgatgtcaa ccgcaatgtc
     1261 ccacgggaac gggtctccgc ccttttctcc gccccgctcc gagtgccgca gcgcgtccgc
     1321 gagactctcc cgcccgaagc gaatgtcgtt gccgacgtgc gcgtcgccga acacccagac
     1381 gttgaattgt cgaccgctca tctgatcccc ctcatatcgc ccaccagtgc tcggggctga
     1441 tgccgggcag ccggaacgcg cggtgctccg gcggcagcga tgccaacaac tcctgcgcct
     1501 tcaggccgat cgcttcggat gccagttcca cgttgcgcct gccaacctcg gcggaggcgt
     1561 gttcgcgcgg gtcgaggccg ccgatgccgt cgggtggctt catgccgggt gcaagggggg
     1621 ccgtgcccag cgccgccatg tccacgaggt ccgggtagaa gaactgcatg ttcgacgtct
     1681 cccatttggc cgcgtggtcg gtgttcgact cctcgcactg gctgaggctg atctcccaca
     1741 ggccgacgcc ggtgatggtg ccgtccgcca ccaccggttt cagcgcctga ttcaccatgt
     1801 caatctgcga ctgcacgttg tggccggata caccgatgat gacacggaac cccgtctgct
     1861 tgagccgctc aaacagcgcg gacagcatgc agaggcgcca gtccgccgcg gcctgctcgg
     1921 gagtgatgtc ggcgggaacg ttgacgtcgt gcgggaagta gacgggcgga tacaccaccc
     1981 cgccgaactt ctccgccgtt ttcacgagga tgccgtgggc tttcagcgcg tcgttgccga
     2041 gcggcaggtg tcggccgtgc cactcgatta ggccaaacgg cacgtacacg accggaaacg
     2101 cgcgggcggc ggcctcaagc tgcgccggtc gcatgaactg catctgcacg gatggggaga
     2161 gggcgttggt catggcgtat gtcctttctg gaacggtacc ttggagtgcg ggagcgccgc
     2221 tcccgctttc atagcgcaaa cgagcttgcg caatccaaag gctggaagtc atccaacacg
     2281 ttcacgctgt cgcatttgaa cgacattaca gtccttctga acccaccttc tgcttccctc
     2341 aaaaaccacc ccatcgaaca ccacggatac gcctcagcgt ctttcacaac ccggtgtttg
     2401 acaggattct ggtgaacgta gtgcaacctc gcgagatagc tcttctcatg ggtcaggcag
     2461 ctatcccagt actgaaacca cacctttcgc cccggggtgc cgtccatttc gttgatccgg
     2521 acggccgttt cggaatgaac tcgtttgatc agtctcgaca aatccgctgc gttttctccc
     2581 cccttgccga cgaagtgata gtggttattc agaatagccc acgcctccag ctgccatccg
     2641 tagtgctttg tttgctcgaa caattctgca agcaggaggt cctttttcgc ggcagtgtcg
     2701 aaaaacggtt gcttcccgta cgtcgctgcg gtgaccatgt aggtgctgcc gggaacgaac
     2761 atgtgcgcgg gcgagtggtg ccactggtgg aaggtttctt gaaggtcgcc cattgcccac
     2821 gcctcttctt tggagtgcgg gagcggcgct cccgctttca cagcgcaagc ccggcttgcg
     2881 cattccaaat tacgacggcg cccttggcaa tcccagccac atccgccaat ttccaatacc
     2941 aacaccagga cgcatcgtga atcgccaaag gacctttccc catgtaccga tttcccgttg
     3001 aacacgtgta tctcgttgac acagttccct ccaacccgca agcccaacgg cgggcgctgc
     3061 ggctggcaac agcactcggt gtgccgtcgc cggaagtgat ctctctggag cgatcgctcg
     3121 agctgcgcca cagccgccag gcgtctcgcc ccggcacgta cccccgccgc acgggggagt
     3181 tcgactactc ccgcgggtgg accgtggttt tggacaccta ctgcgagggc gagcgcttcg
     3241 tggaccgcgt cccccgggcc acctaccgcg acggcgggcg gcagatgggc ggcgagggcg
     3301 tgtgccgctc cgcgctggaa ctccacggcg cgttcgggtg ctaccaccgg tgcgcgtact
     3361 gcttcgtgga cccgtatttc cttatcgcgt gcgacctcga gacgctggca gaccgcatgc
     3421 cggaatatct ggccaggttt ccggcgcaga agctctggaa attcgatgcg ctgacggata
     3481 ccatcgtgct ggaaccggag tacggcgcat cggagctgct cgtgccgctg
//
LOCUS       DAOSOO010000286         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26788_length_3514_cov_2.990171, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000286 DAOSOO010000000
VERSION     DAOSOO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26788_length_3514_cov_2.990171"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(969..2954)
                     /locus_tag="PLT86_13915"
     CDS             complement(969..2954)
                     /locus_tag="PLT86_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOT37753.1"
                     /translation="MAKESTLSRKTQKRRTVGTYKYRDETGQVLFEVVRYEPKNFSMR
                     RPGEHGDWIPNIDSVRRVLYRLPEVIAAVQNNETVYVVEGEKDADRLASLGLCATTNP
                     QGAGQWKAEYSESLEAAHVVILPDNDEAGRRHAEKVAQSLQGKAVSIKVLPLPGLPDK
                     GDVSDWLDLDGANDARKIEELAEAAGKWQPGRGVSDQRDKSEFALTDYGNAERLASLF
                     GHHIRYCWAEKCWYVWSGKRWERDDGGGIQRMAQMTVRKIGKEALRCTDETKRQTIFR
                     WAAASEAVYHMRGMLDLARSLPGVPVLRQEFDTNSHTLNTGNGIVDLYTGELKAHNPS
                     EMVTRITNSDFDPTARHPAWDRLLKTVANRDVGLEGFLQVAIGCSLVAGNVEEKLFLI
                     LGPPATCKTTFLEAIKSALGTYAASASLTAFARRNDPGAARGELVNAQGARLLLSSEA
                     DGDIPLVLSLMKAVTGRDTLTLEKKYQDAVELKPSYTWWIASNESPAFPAGDDGIWRR
                     IVCIPFTHVVPEQDRRPELKESLQRNAVARRAILRWCIEGARLYHQNGLVLPESVRTA
                     TAELRSERDPLSGFISDCCQLGKGNITSSTDLLEASKAWAAVNGKPAPVWGALASTLK
                     RHKCTTTRGSGGVRLWSGVSLVEGVSIPRAETTRCDT"
     gene            complement(2957..3244)
                     /locus_tag="PLT86_13920"
     CDS             complement(2957..3244)
                     /locus_tag="PLT86_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOT37754.1"
                     /translation="MNTSRLKSAIPPAEFFRVELPGMPPTKRANGWVDGGLCPFHDDH
                     NAGNFRINLDNGAFKCFACGARGGDIIAFVMRRRRVSFPNAIAILRNQWGV"
     gene            complement(3241..3480)
                     /locus_tag="PLT86_13925"
     CDS             complement(3241..3480)
                     /locus_tag="PLT86_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37755.1"
                     /translation="MDEAVVAPGQKTKVPRLIPLTKWNEYHPWPPPGGLRWLVFHEKK
                     NGFQAVIKRVGGRVLIDEQAFFDWVDEQNQTGGVR"
BASE COUNT          651 a         1068 c         1034 g          761 t
ORIGIN      
        1 ggaacccgca gcgatgtgca gtggtgcgaa aaaggctccg ggaggcagcc gaggagatgg
       61 ctgacaggac aggctttgac gggggcgtgg gaggcgtctt agatccgttg ccagaaacac
      121 cttgatccgc agaggagtgt gtgggattcc gaaccgccag tactggtccg ccagaggttt
      181 ttgggtgatc ggcctgtcaa acgccgtcgg ggctgaaccg aatgggggca ctggcgggac
      241 cccgcgcgac ggtcggggaa ctggtcagct tcctcacagt tcgcgcgagg cgatctgccc
      301 tgctattaac ccgactgcga aggaatgccg tctcgtctct agaaatccac gcggagcgtg
      361 cgcaagcgct cgcgcgtacg cgcggcgaaa cgacaagcgc ccggatgcgc gtatctccga
      421 tgtcgaacga aggggccggg gaggtcgatc tgctcactga atggggaaaa acggcgtggc
      481 gtgtctgact ttttggcgca ctggcggtcg cagcccgatt ccgcgggggc cgctgggccg
      541 ttttgcggac gcgtcgatgc cctttagaag gccatccgaa ggacctttct gccaacgcca
      601 agggagtcac gataacaaga cttatgtaaa ccttccggtg tggtcggcga gaaaggtcgc
      661 ggcgcgtggt acgctcacaa taccagcact ctcacctgac gcggcgggca ggacagtgtc
      721 ccgatgcccc ccggcgggca cgcgtttggg cgcaggtggg ggtactatct gacagggctc
      781 cgagtttccc cgcttgcacg cgaaaacaca cgttgtgcca aacgcgttcg atcgtcgttc
      841 aggagaccag ggacacgacc tgacgacatc ctgcactgcg gatgcgcagg cccgtgtgac
      901 aggtggtggc acctattcct atgtttgttc cattttcctt cttcagggga tatttgaatc
      961 cggtgtcact aagtgtcaca acgcgtcgtt tcggcccttg gaatcgaaac tccttcaacc
     1021 agggatacgc ccgaccagag gcgcacgccg cccgagccgc gcgttgtagt gcacttgtgc
     1081 cgtttgagtg ttgaagcaag cgcaccccac accggagccg gcttcccatt gacggcagcc
     1141 catgccttcg acgcctcgag taggtctgtg gacgacgtga tgtttccctt cccgagctga
     1201 caacagtcgc tgatgaaacc cgacaacggg tctctttccg agcgcaattc cgcagttgct
     1261 gttctcactg attcggggag aaccagaccg ttctgatgat acagccgggc accttcaatg
     1321 caccaccgga ggatcgcgcg acgcgccaca gcatttcgtt gcagcgactc cttgagttcc
     1381 ggtctgcgat cctgctccgg aacgacgtga gtgaacggta tgcacacgat tcgccgccat
     1441 atcccatcat cgcctgctgg gaaagctgga gattcattgg atgcgatcca ccaggtgtat
     1501 gaaggcttca gttccaccgc gtcctggtat tttttttcca acgtgagcgt gtcgcgtcct
     1561 gtgactgcct tcatcaagct cagaaccaac gggatgtctc cgtcggcttc gctcgacaag
     1621 aggaggcgag cgccttgcgc gttgacaagt tcaccgcgcg ccgcgcccgg gtcgtttcgt
     1681 ctggcaaacg cggtcagact cgctgaggcc gcgtaggttc caagagccga tttgatggcc
     1741 tccaagaagg tggtcttgca tgttgccgga ggaccgagaa tcaagaagag tttctcttcc
     1801 acgttaccgg ccacgagtga acaaccgatg gctacctgga ggaatccctc caacccaacg
     1861 tcccggttcg cgacggtttt gagcaaacgg tcccatgcgg gatgccgcgc ggtcggatcg
     1921 aaatcgctat tggtgattcg ggtgaccatt tcagaagggt tgtgcgcctt cagttcgccg
     1981 gtatacaaat cgacgatccc gttccccgtg ttgagggtgt ggctgttcgt atcgaattcc
     2041 tgtctgagaa cgggcacccc tggaagggat ctcgcaaggt cgagcatccc gcgcatgtgg
     2101 tacaccgctt cggacgctgc cgcccatcgg aaaatggtct ggcgcttcgt ctcgtcggtg
     2161 catcggaggg cctccttgcc gatcttgcga accgtcattt gcgccatccg ctggatccct
     2221 ccaccatcgt cccgctccca tcgctttccg gaccatacat accagcactt ttctgcccag
     2281 caataacgga tgtggtgccc aaagagcgac gcgagtctct ccgcgttccc gtagtcagtc
     2341 aacgcgaact cgctcttgtc tctctggtcg ctgacgccgc gaccgggttg ccacttgcca
     2401 gccgcttcgg caagctcctc gatcttccgg gcatcgttcg cgccgtctaa gtccaaccag
     2461 tcggacacgt cgcccttgtc cggcaaccca ggcagcggaa gcaccttaat tgaaaccgcc
     2521 ttgccctgga gggactgcgc gaccttctcg gcgtgtcggc gcccagcttc atcgttatca
     2581 gggaggatca caacatgcgc tgcttcaagc gactccgaat actcggcttt ccattgtccg
     2641 gcgccctgcg ggttcgtcgt ggcgcaaagt ccaagcgatg cgagacggtc cgcgtccttt
     2701 tcgccttcga ccacgtacac tgtttcgttg ttctggaccg cagcgatcac ctccggcagt
     2761 cggtacagga ccctccgcac gctgtcgatg ttcgggatcc agtcaccgtg ttcgccgggc
     2821 cttcgcatgc tgaagttctt gggctcatag cggactacct cgaacaacac ttgccccgtt
     2881 tcgtcccggt atttgtaagt tcccacggtt ctgcgttttt gcgtttttcg cgaaagggtg
     2941 ctttccttgg ccatggctac actccccact ggttgcggag tatcgctatg gcattaggga
     3001 aactcaccct gcgacgacgc atgacgaacg cgatgatgtc tccgccacgt gcgccgcagg
     3061 caaagcattt gaaggctccg ttgtccagat tgatgcggaa gttgcctgcg ttgtgatcgt
     3121 cgtggaacgg gcacaaaccg ccgtccaccc aaccgttggc acgcttcgtc gggggcatcc
     3181 ccggcaactc gacccggaag aactccgctg gcgggatggc tgatttgagg cgggaggtgt
     3241 tcatcgcacg cctcctgtct ggttctgttc gtccacccag tcgaagaagg cctgctcatc
     3301 gatgagaaca cgccctccca cacgtttgat caccgcttga aagccgtttt tcttctcgtg
     3361 gaacaccaac cagcgcagac ctccaggagg cggccaaggg tggtactcgt tccacttcgt
     3421 gagggggatg agtcggggaa ccttggtctt ctggcctggt gccacaactg cctcgtccat
     3481 tttttagtct ccttcaaagg atgcggcact atcg
//
LOCUS       DAOSOO010000287         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_26824_length_3510_cov_4.762373, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000287 DAOSOO010000000
VERSION     DAOSOO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26824_length_3510_cov_4.762373"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLT86_13930"
     CDS             <1..990
                     /locus_tag="PLT86_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT37756.1"
                     /translation="LLLIRPLRIEEVGALLLTFAFLREQQELSDAAFHGVFHAISAFC
                     NAGFSSFSYSLLPFRDDLLVNVIVMVLIVTGGLGFIVTFELWRLVTGRRRWFKLLLQT
                     KLVLFATLILILSGAVAIFVLEMYGVFGGLGLKSRVLASLFQSVTARTAGFNTVDIAA
                     LSNDTLMILIVLMFIGAAPGSCAGGVKVTTVGVLVLSVLARIHDRPKPEAWNRSVPGD
                     TIARAAALFFGAMLLIAVGTLALQLTELAGVTVEASRAKFLPLLFEVTSAFGTVGLST
                     GITPTLTPAGKIVIIGIMFVGRVGPLTLAATLIGKASRRRAALMYPEEDLMIG"
     gene            996..1652
                     /locus_tag="PLT86_13935"
     CDS             996..1652
                     /locus_tag="PLT86_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT37757.1"
                     /translation="MRSLLMIGLGSFGHHLVSRLAGSNFYIAAIDSDPARVEMVKTVV
                     QKPLIGDASSVDTLKELGVSSFDYVVVSVGDRLDTSILTCLHLRELGAKNIIVKAAHN
                     DHVRLMDMLNVSRVIFPEREAAYQLAHELADLNVLRSIAIEPGISLVEIAVPSEFEGK
                     TLADLGLPTRFGVQVVLIRQLIPEATILPRADFVIKASDTLVIIGTDEAIEKLQQMTT
                     "
     gene            1924..3354
                     /locus_tag="PLT86_13940"
     CDS             1924..3354
                     /locus_tag="PLT86_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37758.1"
                     /translation="MRADGYCFPRAPGFRRCLVATLIALLAGQVPVSAASRTLPAANG
                     MSIVGPWRYQSLWKALRKGRGVIVGTQSGECISGRFVYGGATYVEVSSEDGLREIPVN
                     SIAYVVNVVDLSHARDGAIVGGLIGFGAGVALSYFTRSQTAATPSSASRRARVLDGDE
                     SSDPENGSSTSDPYSDSQSSATTGEDGSSTGADTPSESTAGSDPATGSSGSSTGSDPT
                     ETGSNTYEDGQGSSSGESSGSSAGGGTSPSPTSNSSGSNSSNSNTVVYIGGQGGSSSP
                     RPYNGTDQPAGGSSVSPAARMFFYSVGFAALGTFVGWLIGKRIRNAVPRVDYVLLSPG
                     EAESNGRTPDQYLADKMTSGASGNISESLLPGSSFDSERSAALFDQYAHMGRIVRLHD
                     VLSETITESEAREYRLFPAVRGFTQATIVAYGDSRERQGTELRYFAVVTTMRSGAPLV
                     SLQRLAARDVVRMSTQVRLIRAGILP"
BASE COUNT          671 a          985 c         1078 g          776 t
ORIGIN      
        1 ctgttattga taaggccctt gagaatagaa gaggtgggtg cattgttgtt gaccttcgcg
       61 tttctcaggg aacaacagga attgtcggat gccgcctttc atggtgtttt ccatgcgata
      121 agtgctttct gcaacgcggg tttctcaagc ttcagttaca gcctcctgcc tttccgagat
      181 gatcttctgg tcaacgtgat cgtcatggtg ttgatcgtta ccggcggtct cggatttatc
      241 gttacgtttg agctgtggcg tctcgtcacg ggtcgtcgcc gatggttcaa gctccttctg
      301 cagacgaagc tggttctgtt tgcaaccctg atactcattc tgtccggtgc ggtggccatc
      361 ttcgtgctcg agatgtacgg ggtgttcggg ggattggggc tgaaatcccg cgttcttgct
      421 tcgcttttcc aatctgtgac agcgcggacc gcaggtttca acaccgtgga tatcgcggcc
      481 ttgtccaacg acacgctgat gattctgatc gtgctgatgt tcattggcgc cgctcccggt
      541 tcgtgcgcgg gcggggtcaa agttaccacg gtgggcgttc ttgtgttgtc cgttctcgcc
      601 cggattcatg accgaccgaa gccagaagcg tggaatagga gcgttccggg agacaccatc
      661 gcgcgagcgg cggcgctctt ttttggtgcg atgctgctga tcgcagtggg cacccttgcg
      721 ttgcagctca cggaacttgc gggcgtgacg gtggaagctt cccgcgccaa gttccttccc
      781 ctgctttttg aggtaacatc ggcgtttgga accgtcgggc tgagtacggg gatcacgccg
      841 acgctgacgc ctgcgggaaa aatcgtgatc attggtatca tgtttgtcgg gagagtcgga
      901 ccgttgacgc ttgccgcgac actgatcggg aaggcatcac gcaggcgagc ggcactcatg
      961 taccccgaag aagacttgat gatagggtaa actccatgcg ctcactcttg atgatcggcc
     1021 tcggatcctt cggccaccac ctcgtaagtc gcctggcagg ttccaatttt tatatcgccg
     1081 cgattgattc ggatcccgcg cgggttgaaa tggtgaaaac ggtcgttcaa aagcccctca
     1141 taggcgacgc atcgtccgtt gacacattga aggaactcgg tgtgtcgtcg ttcgactacg
     1201 ttgtcgtaag cgtgggcgac cgactggaca ccagtattct cacgtgtctc catctccgcg
     1261 agctgggcgc caagaacatc atcgtgaagg ccgcgcacaa cgaccatgtc cgactgatgg
     1321 acatgttgaa cgtctcccgg gtgatttttc ccgaacgcga agcagcatac cagcttgctc
     1381 atgagctggc tgacctgaat gttcttcgtt ccatcgcgat tgaacccggt atcagccttg
     1441 tggaaatcgc ggtgccaagc gaatttgagg gaaagacgct cgcggacttg gggcttccca
     1501 cgagattcgg tgtccaggtg gtattgatac ggcagcttat cccggaggcc actattctgc
     1561 cgcgggcaga ttttgtcatc aaggcaagtg acacactcgt gatcatcggt accgatgaag
     1621 caatcgaaaa gttgcagcag atgaccacct gaattccgcc tgttgacaag tatccgaatg
     1681 gatactccgt gcacgcacca cgtgttcgtt tccgcgtgtg caacccgacg ggttcccgcc
     1741 ctgcgcacca caccagtctg aacacgcagt tgccgtctct gtacgtgtct tcccgtttcc
     1801 gtggaaccgg acagaatctg cgctgcggtt tgctggcgaa accatcgtca tgtggcatca
     1861 gttttgcccc acaggacagt ggcacacgcg tgatcacgaa ttctgaccga gggactgaga
     1921 accatgcggg cggacggtta ttgctttccc cgggcacccg gttttcgccg ctgccttgtt
     1981 gcgacgctga tcgcgttgct ggccgggcag gtgccggtga gcgccgccag ccggacacta
     2041 cccgccgcga acggtatgtc gatcgtcggt ccgtggcgtt atcagagcct gtggaaggcg
     2101 ctcaggaaag gtcgcggtgt gatcgtgggg acgcagtcag gcgaatgcat ctccggaaga
     2161 ttcgtgtacg ggggcgccac gtatgtggaa gtgagctcgg aagacgggct acgcgaaatc
     2221 ccggtcaatt cgattgcgta cgtcgtcaac gtcgttgacc tctcccacgc cagagatggc
     2281 gcgattgttg gcggactgat cggcttcggc gccggagttg ccctctcgta cttcacgcga
     2341 agccagacag cagccacccc gtcctccgct tcccgccgag cgagggtact ggacggtgac
     2401 gagagcagtg accccgaaaa cggctccagc accagcgacc catattcgga cagccaatcc
     2461 tcggcaacaa cgggggaaga tggttccagc acgggagcgg acaccccctc ggaatcaacc
     2521 gccggaagcg acccggcaac gggttcgagc ggctcttcta ccggaagcga tccgacagag
     2581 accggctcaa acacctatga ggacgggcaa ggatcgtcaa gcggcgagtc gtctggaagc
     2641 tcggcgggcg gtgggacatc accgtcgccg acgtcaaact catccggcag caattcgagc
     2701 aactcaaaca ctgtcgtgta catcggcgga caaggcggca gcagcagccc gcgtccgtac
     2761 aatggaacgg accagcctgc gggcggatcg agtgtgtctc ccgcggcgcg catgttcttc
     2821 tacagcgtcg gttttgcggc gctgggaacg tttgtcgggt ggcttatcgg gaaaaggatt
     2881 cgaaacgcgg taccgcgggt agattatgtc ctgctttcgc cgggagaagc ggagagcaac
     2941 ggccgaacgc cggaccagta cctcgcggac aagatgactt cgggcgcctc ggggaacatt
     3001 tcggagagtc ttttaccggg cagcagtttt gattccgaac ggagcgcggc ccttttcgac
     3061 cagtacgcgc atatgggaag aatagttcgc ctgcacgacg tgctgagtga aacgatcacg
     3121 gagtcggaag cgcgcgagta ccgtcttttc cccgcggtca gaggtttcac gcaggcaacg
     3181 attgtagcct acggcgattc gcgggaacgc cagggcaccg aactgcgata tttcgccgtt
     3241 gtgacgacta tgcggtcggg tgctcccctc gtatcactgc aacggcttgc cgcgcgtgat
     3301 gtggttcgga tgagtacgca ggttcgcctt attcgcgcgg ggatcctccc atgaggcgat
     3361 ttgccgttct ttgcgcggtg gcggtcgttt tcacgcatgg accggcgcat tcagaggatg
     3421 cgccccagat ggagacgcac tcatctgcgc gcgaaggggc tcctgttctg cctttgcccg
     3481 gtacacggga agggaacttc tcgctggacg
//
LOCUS       DAOSOO010000288         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_27277_length_3464_cov_4.125550, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000288 DAOSOO010000000
VERSION     DAOSOO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27277_length_3464_cov_4.125550"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLT86_13945"
     CDS             complement(<1..639)
                     /locus_tag="PLT86_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT37759.1"
                     /translation="MATFDIPRTRDLLSNFDFQTLFIEVLGWNYPPAHERPRHIDEKG
                     LRYTLEMVSELGGVHVFAVTMDDGDIPPENTRRAIHARVSELSYENLIVFLDRTRTRS
                     LWYWVKREGGVRRPRHAYYFKGQPGDLFISKISSMHVDIGELDEKGNLPITTVVSRLQ
                     KALDIEPVTKKFFIAFDEKRLVFTKHILGIDNEHEQAWYASVLLNRLMFVYFL"
     gene            complement(646..>3464)
                     /locus_tag="PLT86_13950"
     CDS             complement(646..>3464)
                     /locus_tag="PLT86_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT37760.1"
                     /translation="WFEDRWNDRFCLDISDELVRVIGESWASETPLPPYHIYIKMVYH
                     LSQEARAGAIESVIPPDLAKTLFPFQQEAVKIAAHHLRRPERGGVLIGDVVGLGKTLT
                     ATAVLKAFDDLNPLILCPANLVRMWEDYRERWELRGRVLSTGRMLKDLPRLQRYRLLI
                     LDESQNFRNREGKRYRALREYIATNDCKVILLSATPYNKSYRDLGAQLGLFVPEERDL
                     GIRPNRLIASLPGGEKDFVRKYQCSPRTLRAFEASSEPEDWRDLLRMFMVRRTRRFIM
                     ESYATKDESAGRYYLTMENGERAYFPIREPKTVTYPVNEADQFARLYRESVVNMIGGL
                     NLPRYGLGEYVRKDLPADTTETEKEIIDNLGRAARRLLGYCRTNLFKRLESSGHDFLQ
                     SVERHIVRNCVFLYALERGLPLPIGTQDSALLDTAFSDEDIDSLFATVVSPDEDEEST
                     EGTVPPEEALSGDYAARAKRVYDIYRTHMKRQFKWIRADLFESDALIKTLRHDTEEFV
                     RLHRTLPEWDPAQDSKLQALVTLLKTTHPREKVLVFSQFADTVRYLVEELRKAGVRQV
                     EGVTGQSSNPTEAAHRFSPESNGKRDRIRPDDETRVLIATDVLSEGQNLQDCSIVVNY
                     DLPWAIIRLVQRAGRVDRIGQRAPTVRCYTFLPADGVENIIRLRTRLVQRLHQNAEVV
                     GTDEQFFEEERHGKWVADIYHEKAGLLDGEAEDDDTDLTSHAYRIWQQATKDDPELEE
                     AIKRLPDVVYSSKAAPEGANDGVLAYVRTPEGNDVLTCLDAAGNVLTDSPSAILKMAA
                     CEPRTPALPRRPDHHVLVETAVKTIAQEEIRTPGGQLGPPSSPRRRVYERLKTYVERH
                     EGTLFISPDLRKAVHAVYTSPLREEARDILLTRLKSGIGDESLGELVCTLFTQNRLCI
                     TEPDHVASEPQIVCSLGLVASVGE"
BASE COUNT          605 a         1010 c         1065 g          784 t
ORIGIN      
        1 aaggaagtag acgaacataa ggcggttgag gaggacgctg gcgtaccatg cctgctcatg
       61 ctcgttgtcg atgccgagga tgtgcttcgt gaacacgaga cgcttttcgt cgaacgcgat
      121 gaagaacttc tttgtgaccg gttctatatc gagggctttc tgcaggcgcg agacgacggt
      181 cgtgatgggc aggttgccct tctcgtccag ttcgccgatg tccacatgca tcgaactgat
      241 tttgctgatg aacaggtcgc cgggttggcc tttgaagtag tacgcatggc gtggacggcg
      301 cacgccgcct tcccgcttca cccagtacca gaggctgcgc gtccttgtcc tgtcgagaaa
      361 cacgataagg ttctcgtaac tcagttcgga tacgcgcgcg tggatcgcgc gtcgcgtgtt
      421 ttcgggtggt atatcgccat cgtccatggt gacggcgaat acgtggacgc cgcccaattc
      481 gctcaccatc tcaagggtat accggagtcc tttttcgtcg atgtgccgcg gtctctcgtg
      541 tgcgggcgga taattccagc cgaggacctc gatgaacagg gtctggaagt caaaattgct
      601 cagcaggtct cgcgtacgag ggatgtcgaa cgttgccatg gcggcctact ctcccacgga
      661 ggcaacaagc cccagcgaac agacgatctg cggttcggag gctacgtgat caggttcggt
      721 gatgcaaagg cgattctggg tgaacagcgt gcagacgagt tccccgaggc tttcgtcccc
      781 aatgcccgat ttcagacgcg tgagcaggat atcgcgcgct tcttcccgca aaggggatgt
      841 atagacggcg tgcaccgcct tgcgcaagtc gggcgaaatg aacagcgtgc cctcgtggcg
      901 ctcgacgtat gttttcagac gctcgtacac gcgacgccgc gggctggagg gcggcccgag
      961 ttgtcctccc ggcgttctga tctcctcctg tgcgatcgtt ttcaccgctg tttcgaccag
     1021 cacgtggtga tcggggcggc gtggcaaggc gggagtgcgg ggctcgcacg cggccatttt
     1081 caggattgcg gacggggaat ccgtaagcac gttgcctgcg gcgtccagac aggtgagcac
     1141 atcgtttcct tccggcgttc ggacatatgc cagcacgccg tcgttcgcgc cttcaggggc
     1201 tgccttgctg gaatagacca catcagggag tcgcttgatg gcctcctcca gttccggatc
     1261 gtctttcgtt gcttgctgcc aaatccggta cgcatgggat gtgagatcgg tgtcgtcgtc
     1321 ctcggcttcg ccgtcaagca ggccggcttt ctcgtggtag atatcggcaa cccatttccc
     1381 gtgccgttct tcctcgaaga actgctcgtc ggtgcccacg acttcggcgt tctggtggag
     1441 cctctgcaca agacgcgtgc gcaggcgaat gatgttctca acaccatccg cgggcaggaa
     1501 tgtgtaacag cgaacagttg gtgcccgttg cccgatcctg tccacacggc ctgcccgctg
     1561 cacgaggcgg ataattgccc aggggaggtc gtaattcacg acgatactgc aatcctggag
     1621 gttctgccct tcgctcagca catccgtggc gatgagaacg cgggtttcgt cgtccggccg
     1681 gatgcggtct cgtttcccgt tgctctcggg gctgaaccga tgcgccgctt ccgtggggtt
     1741 tgacgattga ccggtgacgc cctcgacctg ccgcacgcct gcctttcgca gctcctcaac
     1801 gagatagcgc acggtatcgg cgaactggga gaagacgagc accttttccc gcgggtgtgt
     1861 ggtcttgagc agcgttacaa gagcctgcaa tttggagtcc tgagccgggt cccactcggg
     1921 gagggttctg tgcaggcgga cgaactcctc ggtgtcgtgc cgcagcgttt tgatcagtgc
     1981 atccgattcg aacagatcag ctctgatcca tttgaattgc ctcttcatat gcgtccggta
     2041 gatatcgtac actcgcttcg cacgcgcggc gtagtctccg ctgagagcct cctccggcgg
     2101 taccgttccc tcagtggact cttcgtcttc atcgggggaa acgactgttg caaacaggga
     2161 gtctatgtcc tcgtcgctga acgcggtatc gagcagcgcg gaatcctggg tgccgatggg
     2221 cagcggcaat cccctttcga gtgcgtacaa gaagacgcag ttccgcacga tgtggcgttc
     2281 cacggactga aggaagtcgt gcccgctgct ttcgaggcgc ttgaacaggt tggtgcggca
     2341 gtaccccagc aggcgccggg ccgctcgacc caggttgtct atgatctctt tttcggtttc
     2401 cgtggtgtct gcgggcaagt ccttccgcac gtattccccg aggccgtagc gcggcaggtt
     2461 caggccacca atcatgttga cgacggattc ccggtacaaa cgcgcgaact ggtccgcctc
     2521 gttgacgggg tacgtgacgg ttttcggttc cctgatcggg aagtacgccc gttccccgtt
     2581 ttccatggtg aggtagtatc ttccggcgct ctcgtccttc gtggcgtagc tctccatgat
     2641 gaacctgcga gtgcggcgca ccatgaacat gcgcaggagg tcacgccagt cctccggctc
     2701 gctgctcgct tcaaacgcgc gcagcgttct cggcgagcac tggtacttgc ggacgaagtc
     2761 cttctccccg ccggggagcg acgcgatcag cctgttcggg cggatgccga ggtcgcgctc
     2821 ttccggaacg aagagaccga gttgtgcgcc gagatcccgg tagctcttgt tatacggggt
     2881 ggccgagagc aggatgactt tgcagtcgtt tgtggcgatg tactcccgca gtgcgcggta
     2941 cctcttgccc tcgcggttgc ggaagttctg gctctcgtcc agtatgagga gccggtaccg
     3001 ctggagtcgt ggcagatctt ttagcattct gccggtggac aggactctcc ctcgaagctc
     3061 ccaacgttcc cggtaatctt cccacatacg aacgaggttt gccgggcaca agatgagcgg
     3121 gttaaggtcg tcaaacgcct tcaaaactgc cgttgcggtg agtgtctttc ccagtcctac
     3181 cacatcgccg atcaacacgc cgccgcgttc cggccttctc aggtggtgcg cagcgatttt
     3241 cacggcttcc tgctgaaacg ggaaaagcgt ttttgcgagg tccggaggaa tgactgattc
     3301 aatcgctccc gcgcgcgctt cctgcgaaag gtggtatacc atcttgatgt agatgtggta
     3361 cggcggcagg ggcgtttcgc tggcccagct ctcccctatc acgcggacaa gctcatcgga
     3421 gatgtcaaga cagaagcggt cgttccatcg gtcttcaaac catc
//
LOCUS       DAOSOO010000289         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_27807_length_3414_cov_4.811253, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000289 DAOSOO010000000
VERSION     DAOSOO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27807_length_3414_cov_4.811253"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..3205
                     /gene="glnE"
                     /locus_tag="PLT86_13955"
     CDS             119..3205
                     /gene="glnE"
                     /locus_tag="PLT86_13955"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOT37761.1"
                     /translation="MPDPGKTLQNVLLGVFPELSPDSCTELFVRHFPQHQERVSRAIE
                     TLVGLLPSQTVRLLVETCVVSPDPGAAISCLSELVQAAPSSFRDVAPEQARRLASSLS
                     GGAFLRERILRHATRVFWLVDESGRTFSRPELEQEIAETREGSAVWDALRVWHDLHLM
                     RIAVADCAGTLGIVEITEQLSLLAEVTINEVAQRQAQALRARYGDPLQQDGANAEWCV
                     IGLGKLGGNELNFRSDVDVMFVYSAEGYSTGGTSGAVPVAQWYTRWAEGILSVLTEVS
                     STGLLYRVDTRLRPDGQSGALVRSIASYVHYYEQRGEVWERQMLVKARPVAGSVRMGQ
                     KLLDFLESFIYPRSLVTSPREEIHRVKNRILQHLDARTTSSNPANGERNLKLGRGGLR
                     DIEFVVQCLQLVVGGSDKAVREQNTLAAIRRLHQRGVLSDTEQRALDEAYRLFRRTEH
                     RLQMATGQPSFVLPSEHDELSLLARRMGYSDPAALMADLETARKNVMSVYDSVLGPPE
                     TGTEIGKILNLPAGDAQVVSELAGCGFRNPEAAHRNLLHLAFGHGDTVPASTPSQPVL
                     RLVPRLLDELRGSANPDRGLNNVERMLRAFGAVDSFADLLSSHNAFLNLLVTIAARSE
                     ALTETISRDPGLVDWMLYSGALFAERHPEDVELESRAAVAGLPSAALQIAALHSFRKR
                     ENLRVGVRFLMDLANEEETSEQLTAIAESVVKRVVSVASRSLAGAAAENRSYQWAIIG
                     LGKLGGREMNFGSDLDIFFVHAEEQDEGQCVDYVTSLAQEVLRLLCENTEYGVLYEVD
                     TRLRPEGKHGPLSLSIDGFREYLRTRAAQWERQALTFSRVIVSAPQPRFGEVVEEAIW
                     NAIAVKPTREIFESALSMRLRMQEQAQSNYGTRPNIKTGFGGLVDAEFIAQLGRMIGG
                     VRLSEGRKGDTRATLECMRSAGLLPDRVAQTLMDAYHALRRIQMHLRVNDYRAHNVLP
                     DDEDAILTLARGLGFRDRSDLSVHVEKVTEDMRGAFLLALESLATANTKALGGSFV"
     gene            3198..>3414
                     /locus_tag="PLT86_13960"
     CDS             3198..>3414
                     /locus_tag="PLT86_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37762.1"
                     /translation="MSDRFMGGWRALAILIAAVISLATGPALSAAEETRNLRIGPIAE
                     VRVSPQFSVSLVIQRRGRSLQELGKHFL"
BASE COUNT          702 a          949 c         1058 g          705 t
ORIGIN      
        1 aaccgagtgg taagcgcggg ttctcctctc cggcatcgct gaggaggtcc tcgtcagcga
       61 tgccgcgtgc tgttccccgc cacccgttct tccggcccgc cagggggctc ttttgaaaat
      121 gcctgacccc ggcaaaactc tacagaatgt cctgctcggg gttttcccgg agctctcccc
      181 tgatagctgc acagaactgt tcgtgcggca tttcccgcaa catcaagaac gcgtctcccg
      241 tgcaattgag actcttgtgg ggttgcttcc ttcgcagacg gtgcgcctgc tggtggaaac
      301 ttgtgtggtt tcccccgatc ccggcgccgc gatctcctgt ttgtctgaac ttgttcaagc
      361 ggcaccttcc tcgttccgtg acgtggcccc tgaacaggcg agaaggctgg cctcgtccct
      421 ctcaggtggg gcctttctca gggaacggat cctcagacac gcaacgcgag tcttctggtt
      481 ggttgatgaa tcgggacgga cgttctcccg gccggaactc gaacaagaaa tcgcggagac
      541 ccgcgaggga agcgccgtgt gggacgcact gcgcgtctgg cacgatctcc acctgatgcg
      601 tattgccgtt gcagattgtg ccggcacgct gggtatcgtg gagataacag aacaactttc
      661 tctccttgcc gaggtgacga tcaatgaggt tgcccagcgg caggcgcagg cgctcagagc
      721 gcggtacgga gatccgctcc aacaggacgg cgcaaacgcg gaatggtgtg tgatcggctt
      781 gggcaagttg gggggaaacg aactcaactt ccgttcagac gtggacgtga tgttcgtata
      841 ctccgcggaa ggatactcga cgggaggaac ttccggggcg gtaccggtgg ctcagtggta
      901 cacccggtgg gcagaaggaa tattgagtgt ccttacggaa gtaagctcga cggggctgct
      961 gtaccgcgtc gatacgcgtc tccgtccaga tgggcagtct ggcgcattgg tacgcagcat
     1021 tgcctcgtac gtccattatt acgagcagag gggcgaggtc tgggagaggc agatgctcgt
     1081 caaagcgcgt cccgtcgcag gctcagttcg catggggcaa aagctgctcg attttctgga
     1141 gtcctttatt tacccgcgaa gcctcgtaac aagtccacgg gaggaaatcc acagggtcaa
     1201 gaatcggatt ttgcaacatc ttgacgcgag aacaaccagt tcaaacccgg cgaatggaga
     1261 acggaacctc aagctgggcc ggggggggct gcgagacatc gagttcgtgg ttcagtgtct
     1321 tcaactggtc gtagggggca gcgacaaagc cgtacgggaa cagaacacgc tcgcggcgat
     1381 caggagactc caccagcgtg gtgtgctaag tgacactgag caacgagcgc tggatgaggc
     1441 ataccggctc ttccgacgca cggaacatcg tctgcagatg gcgacagggc agccatcatt
     1501 cgtccttcct tcagagcatg acgagctatc acttctggcg cgaagaatgg gttactctga
     1561 tccagccgcg ttgatggcag acctggaaac ggccaggaag aacgtcatgt cggtatatga
     1621 cagcgttctg ggcccgccgg agacaggcac agaaatcggc aagattctga atctccccgc
     1681 aggcgatgcc caggttgtct cggaattggc cgggtgcggt tttcgcaacc ccgaggcggc
     1741 gcaccggaat ctgctccacc ttgcgttcgg tcatggcgat acggttcctg cttccacgcc
     1801 ttcccagccc gtgctccggc tggtgcctcg tttgctggat gaactccgtg ggagtgcaaa
     1861 cccggatcgg gggctcaaca acgtcgagag gatgctcaga gcgtttggcg cagtagacag
     1921 cttcgctgac cttctgtcgt cgcacaacgc attcctcaac ctgctagtaa caatcgcagc
     1981 acgcagcgaa gctctcaccg aaacgatctc cagggatcct ggtctcgtgg attggatgtt
     2041 gtattccggc gccctcttcg ccgaaaggca cccggaagac gtcgaattgg aatcccgggc
     2101 ggccgttgcg ggtctgccca gtgctgcatt gcagattgcc gcacttcatt cgtttcgaaa
     2161 gcgcgagaac ctgcgtgtgg gagtccgttt cctcatggac ctcgcgaacg aagaggagac
     2221 atctgagcag ctcactgcca tcgcggaatc ggtggtgaag cgggttgtct ctgtcgcgag
     2281 cagatcgttg gcgggggcag ctgcagaaaa ccgttcctac cagtgggcaa tcatcggctt
     2341 gggaaaactg ggcggacgcg aaatgaactt cgggagcgat ctcgacattt tcttcgtaca
     2401 cgcagaagag caagacgaag gccaatgtgt ggactatgtt acttcgctcg cgcaggaggt
     2461 tctccggctc ctgtgcgaaa acacggagta cggggttctt tatgaagtcg acaccaggct
     2521 tcgtcccgaa ggaaagcacg gtccattaag cctttcgatc gacggtttcc gcgagtacct
     2581 tcgcacacgt gcggcacagt gggagcgtca ggcactgacc ttctcacgcg tgatcgtttc
     2641 cgcgccgcag ccgcgcttcg gagaagttgt cgaggaagca atctggaacg cgattgcggt
     2701 gaaacccacg agggagatat ttgagagcgc gctttcgatg cggttgcgca tgcaggaaca
     2761 ggcacagagc aactatggaa cgcggccaaa cataaagacc ggcttcggcg ggctggtgga
     2821 cgcagagttc attgcgcaat tggggaggat gatcggcgga gtacgccttt cggaagggcg
     2881 aaaaggcgac accagagcca ctctcgaatg catgagatct gcaggccttc ttccagaccg
     2941 ggttgcccag acgctgatgg atgcgtacca cgcgttacgg cggatccaga tgcatcttcg
     3001 cgtgaacgac tatcgcgcgc acaatgttct gcccgacgat gaagacgcta ttctgaccct
     3061 tgcaagaggg ttgggtttcc gggacaggag tgatctttcc gtgcacgttg aaaaagtgac
     3121 ggaagacatg cgcggtgcgt tccttttagc cctcgaatcc ctcgcgaccg cgaatacaaa
     3181 agcactcgga ggatcgtttg tctgaccgat tcatgggcgg atggcgcgca ctcgcaatcc
     3241 tgattgccgc agtgatctca ctcgcaacgg gtccagccct ctcggcggcg gaagagacgc
     3301 gtaatctccg gatcggtccc attgcggagg tccgggtctc gccgcaattc tcggtgtctc
     3361 tggtgatcca gcggagaggg cgctccttac aggaactggg gaagcatttc cttg
//
LOCUS       DAOSOO010000290         3374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_28237_length_3374_cov_4.175655, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000290 DAOSOO010000000
VERSION     DAOSOO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28237_length_3374_cov_4.175655"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLT86_13965"
     CDS             <1..444
                     /locus_tag="PLT86_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT37763.1"
                     /translation="VNISARALETGHFETFRAALEGIISELVDDDTRKGVPPERRFTA
                     DMDLLKWAPPMVCDTAGVWYRTVARIAGQEKPEFVTYGTDAGVLNQVGLPCVVWGPGD
                     IIRAHSVDEYITLEELTTAVTHYRELICAVAASELPTFTTSLWNG"
     gene            complement(409..2460)
                     /locus_tag="PLT86_13970"
     CDS             complement(409..2460)
                     /locus_tag="PLT86_13970"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT37764.1"
                     /translation="MKNSDGPIIGAICLNPGNDQAIETLRHIASLSMTMCETILVRGD
                     TAPARVLEIRRFLDEAQKLGVRVLLEPTYEPDLSRHLGMRNIGADGKISTRDNGFCYR
                     NKDYLAELSVFVAEVAQLYGDHPGILRVGGRPVIAIGHEMQYQITPQDGHGGAVRTMT
                     CYCGYCLHGFREWLRSLYGTISAFNRVHSRQVLSFGRVTPPRKPEPDCRLWQEWVDYH
                     ATAIPECIALQRQIIERELRGALVTHEINDWYPNTWDCVYTGNHFWKMGEKLEHSFND
                     QYPMEWAPGSLWRIYLYTFTQDVNQSAMGFERSFWTNGQAFPSWQGDFTEPPDAGYHE
                     QVYSALIHGANGLIWWWGGDYLRRSEAASREYAKLVRIVGNTRPAKDPVAILAPWTTF
                     AQTRSSDRGDDLMGGYQLLIRLGFQVEVVDERQVSEGVLASRGYRALITWGNSCVSPE
                     ARKEIEDFATRGGLVLADFGDQNVAPFGTIFPRTISRNPTRARAYVLPDGTTVAVRSG
                     AQALAAGKRVEVIARFDDGRPAIVRQPIGSGVLWRAGSFVGLDYAEGMGVYDWVQQQR
                     VRIEPALENLIAEALERQGLRPIGSTNNRNAEVGIFPVDSRLLVLVANHLRHPLQVTV
                     RVHISGWTSAKDVFSGEIAGCIQERDVTAFPVRLPEMGGRAFWLMRQTSPVPERRGES
                     R"
     gene            2616..2709
                     /locus_tag="PLT86_13975"
     tRNA            2616..2709
                     /locus_tag="PLT86_13975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ser,seq:gct)
     gene            2777..3187
                     /locus_tag="PLT86_13980"
     CDS             2777..3187
                     /locus_tag="PLT86_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37765.1"
                     /translation="MRLVITRVHVWPAAKVGAVLGCLSGLIFGVTFVLIGSALSPLGQ
                     ILDQPVNGIGVSGVVLITLANGSLWCSTFAAGAALYNLAARLGANVAFTAKSPAEEAP
                     SGQESSTERNDPSGLDNCSFATRTFKGGSGEHTS"
     gene            3203..>3374
                     /locus_tag="PLT86_13985"
     CDS             3203..>3374
                     /locus_tag="PLT86_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT37766.1"
                     /translation="MGQSSVRVRFAPSPTGYLHIGGARTALFNWLFARHEKGTFILRI
                     EDTDQTRVVEGAI"
BASE COUNT          689 a         1004 c          962 g          719 t
ORIGIN      
        1 gtcaatatta gcgcccgagc actcgaaacg ggacactttg aaacattccg agcggcactg
       61 gaagggatca tcagcgagct tgtcgacgac gacacacgaa agggagttcc tccggaacgg
      121 cggttcacag cggacatgga cctgctgaag tgggccccgc cgatggtgtg cgatactgca
      181 ggcgtgtggt accggacagt tgcacggatc gccggacagg agaagccgga atttgtcacc
      241 tacggaactg acgcgggagt gttgaaccag gtcggccttc catgcgtcgt atggggaccg
      301 ggtgacatca tccgcgctca ttccgttgac gagtacatca cactggaaga gcttacaact
      361 gctgttaccc attaccgcga actcatctgt gcggttgctg cctccgagct accgactttc
      421 accacgtcgc tctggaacgg gtgaggtttg gcgcatcaac cagaaggccc gtcctcccat
      481 ctccggcagc cgcaccggaa aagccgtaac atccctctcc tgaatgcatc cggcgatttc
      541 acctgagaag acatcctttg ccgaggtcca ccccgaaata tgcacgcgca cagtgacctg
      601 taacggatgg cggaggtggt ttgcgacgag aaccaaaagc cgcgagtcga ccgggaagat
      661 ccccacctcc gcgtttcggt tgttcgttga cccgatcggt cgcaagccct gtcgttcgag
      721 cgcttccgct atcagattct ccagggcagg ttcaattctt acgcgctgtt gctggaccca
      781 atcgtacacg cccattcctt ccgcataatc caacccgacg aacgaaccag cgcgccacag
      841 cacgccgctt ccgatcggct gtcgcacgat cgccgggcgc ccgtcgtcga aacgcgcgat
      901 gacttccaca cgtttcccgg cggcgagagc ctgtgctccc gagcgcacgg ccaccgtcgt
      961 tccgtccggc agtacgtacg cccgcgcccg cgttggattc cgggaaatcg tcctcggaaa
     1021 gatggtgcca aaaggcgcaa cgttctgatc cccgaagtcg gcaagaacaa gtcctccgcg
     1081 cgtagcgaaa tcttcgattt ctttccgcgc ctctggcgaa acgcatgagt tgccccaggt
     1141 tatgagcgcc ctgtaaccgc gcgatgcgag aacaccttct gaaacctgcc gctcgtccac
     1201 aacttccacc tggaatccga gtctgatgag aagttgatac ccgcccatca gatcgtcacc
     1261 acggtcggat gatcgcgtct gtgcgaacgt ggtccatggc gcaagaatcg caacgggatc
     1321 cttcgcaggc cgcgtgtttc ccactatccg gaccaacttc gcgtattcac ggcttgccgc
     1381 ttctgaacgc cgcagataat cccctcccca ccaccagatc aggccgttcg caccatggat
     1441 aagtgcgctg tacacctgtt cgtggtagcc cgcatcgggt ggttcggtga aatccccctg
     1501 ccacgaagga aatgcttgcc cattcgtcca gaacgagcgt tcaaagccca tcgcgctctg
     1561 gttgacgtcc tgagtaaacg tgtacaggta gatccgccaa agtgatccag gcgcccattc
     1621 catggggtac tggtcgttaa aagaatgttc gagcttttcg cccatcttcc agaaatggtt
     1681 cccggtgtac acgcagtccc acgtatttgg gtaccagtca ttgatctcgt gggtcacaag
     1741 ggcaccacga agttctcgtt cgatgatttg ccgttgcagc gcaatgcatt ccggaatcgc
     1801 cgttgcgtgg tagtccaccc attcctgcca aaggcggcaa tcgggttccg gttttcgagg
     1861 tggcgtcacg cggccaaaag aaagcacctg gcggctgtgg actctgttga acgcagaaat
     1921 ggttccgtac aaactgcgca accattcccg gaagccgtgg agacaatatc cgcagtaaca
     1981 tgtcatcgta cgaaccgcgc ctccgtgtcc gtcttgaggc gttatctgat actgcatctc
     2041 atgtccgatc gcgatgaccg ggcggccccc gacccgaagg ataccggggt ggtcgccata
     2101 caactgcgcg acctcggcca caaagaccga gagctctgcg aggtagtcct tgttgcggta
     2161 acaaaatccg ttgtcgcgtg tgctgatttt cccgtcggca ccaatgttcc gcatccctag
     2221 atgccgggag aggtctggtt cgtacgtcgg ttcgaggagg actctcacac ccagtttttg
     2281 cgcctcatcg aggaatcggc gtatctcaag cacgcgcgca ggcgcggtat cccctcgaac
     2341 gaggatcgtt tcgcacattg tcattgaaag agacgcgatg tggcgcagtg tctcaattgc
     2401 ttggtcgttg ccgggattga ggcagatagc gccgatgatt ggaccgtccg agttcttcat
     2461 ccgatgtctc cggtggggca gcgtgttatt aagtcattgc aaggatctgt cagtacaata
     2521 ggcatgcgac ggcgtgacac caagcaagga ctcttcctcg tcttgacaca ttcctcgtgc
     2581 ttgccgtaac ttcattgtct gatttccggc tgaacggaga ggtgaccgag ctggccgatg
     2641 gtgctcgcct gctaagcgag tgtggggtca aaagccccac cgcgggttca aatcccgccc
     2701 tctccgccat atttcctgcg cgtgcctctc cacccagata tcggtggaga ggcattgacg
     2761 tctgagggtt cgactgatgc gcctggtgat aacgcgtgtt cacgtgtggc cggcggccaa
     2821 agtgggcgca gtgctggggt gcctgtcagg actgattttc ggcgtaacat ttgttttgat
     2881 cggatcagcg ctctccccgc tggggcagat cctcgaccag cctgtgaacg ggattggcgt
     2941 ttccggcgtg gtactgatca cccttgcgaa cgggagcctt tggtgctcga cgtttgctgc
     3001 gggtgcagcg ctttacaacc tggcagcgcg gttgggcgcg aatgtggcct tcactgcaaa
     3061 gagtccggca gaggaagctc cgtcaggtca ggaatcctcc acggaacgca acgatccctc
     3121 cgggttggac aactgctcgt ttgccaccag gacattcaaa ggtgggtccg gcgagcatac
     3181 gtcgtgaaat gaaagcgtga aaatgggaca atcatcggta cgcgttcgct ttgctcccag
     3241 tccgaccggg tatctgcaca tcggcggggc ccgcacggcc cttttcaact ggctttttgc
     3301 gagacacgaa aagggaacat tcatcctccg aatcgaggac acggaccaga cgcgagtggt
     3361 tgagggcgca attg
//
LOCUS       DAOSOO010000291         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_28358_length_3362_cov_6.275174, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000291 DAOSOO010000000
VERSION     DAOSOO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28358_length_3362_cov_6.275174"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLT86_13990"
     CDS             <1..400
                     /locus_tag="PLT86_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37767.1"
                     /translation="RHATPYSLVLDPNPNKRKDELVLAFPAQYVEPELPAVAAARDTV
                     TADSAEPAGEAESTAPMDSAQAGVQTPPVAPKPAATVDSAPAPPPPAAEDVPAPVADQ
                     PAGTADTTAPVAEPESEQPPAPVESPQNGQ"
     gene            548..1048
                     /locus_tag="PLT86_13995"
     CDS             548..1048
                     /locus_tag="PLT86_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT37768.1"
                     /translation="MSAQNSDIPADVAKLLAQFARLSQRFSDQDEIDADMIRLLQQFM
                     GAKRPFHGFSEHVWRPPTDVYETEDSMVVKVEIAGVEKDAFHLEFSRNVLIVRGRRSQ
                     KLGISQVSYHRMEIKYGEFEVEFALPLGLDQDRTTAHYRDGFLTITIPKEDTQITRSV
                     SIDIQG"
     gene            1054..>3362
                     /gene="lon"
                     /locus_tag="PLT86_14000"
     CDS             1054..>3362
                     /gene="lon"
                     /locus_tag="PLT86_14000"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT37769.1"
                     /translation="MPDSQDTIPFHTPDSQAPEFPETLGVLMREDLLIFPLMIAQLQI
                     REASTTRLIDEALSTHRIIGVLAAPAPEGDRPALPELPRIGSAVVIHKMLKLPDGTVS
                     ILVQGVARVRFTEFLQTKPYVLARGTRLESETDDSVETQALARNVTELFQRLIGLVPQ
                     LPDEWKAVVQTMSRNPDHLSDFVAANINITNENRQKLLETLVVRERLSHLSTLLNNEL
                     EVVELSNKIQGQVQSEMSKNQREFFLRKQLETIRQELGEEDEQTAEINELQKKLEEAN
                     LPQQAKEAAAREIDRLRRMPPAAAEYTVARTYLDWLINIPWSVSTEDNLDIRRAKKIL
                     EADHYGLAKIKDRILEYLAVRKLKADMKGPIICFVGPPGVGKTSLGQSIAHALGRKFV
                     RLSLGGIHDEAEIRGHRRTYIGALPGRIVQGLRRAGSNNPVFMLDEVDKIGQDFRGDP
                     SSALLEVLDPEQNSAFSDHYLEVDVDLSKVMFITTANQLAPIPPPLRDRMEVLELPGY
                     TEEEKVQIAKRHLLPRQREAHGLTAEQISFNDAAVRAIIGSYTREAGVRNLEREIANI
                     CRKVARKIAEGEKPPFVVGKKEVEEYLGPARFDSETAERMLDVGVVTGLAATTVGGDI
                     IFVEATKMPGTGKLILTGQLGDVMRESAQAALSYVQSQQKKYHIEDVDFSKTDIHIHV
                     PAGATPKEGPSAGVTLVTALVSLLTGRRARGDVAMTGEITLRGKVMPIGGVKEKTLAA
                     QRAGIHTVILPARNKRDLEDIPKTVRKKVT"
BASE COUNT          730 a          964 c         1057 g          611 t
ORIGIN      
        1 gcggcatgcg acgccgtaca gccttgttct ggacccaaac ccgaacaagc gcaaggatga
       61 actggttctg gcgtttccgg cgcagtatgt ggagcctgaa ctgcctgcgg tggccgcggc
      121 tcgtgacaca gtgactgccg attcggcgga acctgcgggt gaagcggaga gcactgcgcc
      181 gatggattcg gctcaggcgg gtgttcagac gccgcctgtt gcaccgaagc ccgcggcaac
      241 cgttgactcg gcacccgcgc ctcctccacc tgccgcagag gacgtaccgg cgcccgtggc
      301 agatcagccc gccggtacgg cagacaccac ggcgcccgtg gcggaaccgg aatccgaaca
      361 gcctcccgcg ccggtggaaa gcccccagaa cgggcagtag gctgtatcaa tccttctttg
      421 gcacagctat tgcgcttgta tacccatttc agggattgtc cctttttgcc cgggacagcc
      481 tctgctcctg cgcccgggcc cgggccactg ccatgacccg gctgcttttc ctgagaagga
      541 gtgtgtcgtg tcagcacaga attcggatat tcccgcggac gtcgcaaagc tgctcgctca
      601 gtttgcgcgc ctttcgcagc gtttttcgga ccaggatgag atagacgcgg acatgatccg
      661 cctgctccag cagttcatgg gagcgaaaag gccattccat ggcttttccg aacacgtgtg
      721 gcgtcctccg accgacgtgt atgaaacgga ggactccatg gtcgtgaagg ttgagattgc
      781 cggcgtggag aaggatgcgt ttcatctgga gttttcccgc aacgtgctga ttgtgcgtgg
      841 ccgcaggagc cagaagctcg gcatctccca ggtgagttac caccgaatgg agatcaaata
      901 cggggagttc gaggtggaat tcgcgcttcc gctgggattg gatcaggacc gcaccacggc
      961 acactaccgc gatgggttcc tgaccatcac gatcccgaaa gaggacacgc agataaccag
     1021 aagcgtctcg attgacatac aggggtaatt tgcatgccag attcacagga cacaattcca
     1081 ttccatacac cggacagcca ggcacccgag ttcccggaaa cactcggcgt tctgatgcgg
     1141 gaggatcttc tgattttccc cctcatgatc gcgcagttgc agatccgcga agcgtcgacg
     1201 acccgcctga ttgacgaggc actttcgacg caccgcatca tcggggtgct tgcggcgccg
     1261 gctccggaag gggacaggcc ggcacttccg gaactgccgc gcatagggtc ggcagtggtt
     1321 atccacaaga tgctcaaact gcccgatggc acggtaagca ttctggtgca gggagttgcc
     1381 cgcgtccgct tcacggaatt cctccagacg aaaccgtatg tgctggcgcg gggcacacgt
     1441 ctggaaagcg aaaccgacga ttcggtagaa acgcaggcgt tggctcggaa tgtcacggag
     1501 cttttccagc ggcttatcgg gctggtgcct cagcttccgg acgagtggaa ggccgtagtt
     1561 cagacaatga gccgcaaccc cgaccatttg tcggatttcg ttgccgccaa catcaacatc
     1621 accaacgaaa acaggcagaa gctgctggaa acgctcgttg tgcgggagcg gctgtcccat
     1681 ctctccacgc ttctcaacaa cgagcttgag gttgtggagc tgagcaataa gatccaggga
     1741 caggtccaga gcgagatgag caagaaccag cgggagtttt tcctgcgcaa gcagctcgag
     1801 acgatccggc aggaactcgg agaggaagac gaacagaccg ccgaaatcaa cgagctgcag
     1861 aaaaagctgg aggaggcgaa ccttccgcag caggcgaagg aggccgcggc gcgcgaaatc
     1921 gaccgtctcc gcaggatgcc gcccgccgcc gcggaataca ccgttgcccg gacgtacctg
     1981 gactggttga tcaacatccc gtggtccgta tccacggagg acaatctgga tatccgcagg
     2041 gcgaagaaga tcctcgaagc tgatcattac gggctcgcga agatcaagga ccgcatcctc
     2101 gagtacctcg cggtgcgaaa actgaaggcg gatatgaagg ggccaattat ctgttttgtc
     2161 ggccctcccg gagtgggtaa gacgtcgctc gggcagtcaa tcgcgcatgc cctcggccgg
     2221 aaattcgtcc gcctgagcct tggcggtatc cacgatgaag cagaaatccg cgggcacaga
     2281 cggacgtaca tcggtgcgct tccgggccgc attgtgcagg ggctccgccg tgcgggaagc
     2341 aacaatccgg tgttcatgct tgatgaagtg gacaaaatcg ggcaggattt ccgtggtgat
     2401 ccctccagtg cacttctcga agtgctcgat cccgagcaga actcggcctt cagcgaccac
     2461 tatctcgaag tggacgtgga tctttcaaaa gtcatgttca tcacgacggc gaaccagctc
     2521 gcgcctattc cgccgccact gcgggatcgt atggaggtgc ttgagcttcc ggggtatacg
     2581 gaggaggaaa aggttcagat cgccaaacgg cacctccttc cccggcagag ggaggcgcac
     2641 ggcctgactg cggagcagat atcctttaac gatgcggcgg tgcgtgccat tatcggcagt
     2701 tacacccggg aggccggggt ccggaatctc gagcgggaga tcgcgaatat ctgccgcaaa
     2761 gtggcgcgga agatcgcgga gggagagaag ccaccgtttg tcgtcgggaa aaaggaagtg
     2821 gaggagtacc tcgggcctgc ccgattcgac tccgaaacgg cggaacgcat gctggatgtg
     2881 ggtgtcgtta cggggctcgc agccaccacg gtgggaggcg acatcatttt cgtcgaagcg
     2941 acgaagatgc ccggcaccgg aaaactgatc ctgacgggcc aacttggtga tgtgatgcgc
     3001 gaatcggcac aggcggcgct ttcgtacgtg cagtcgcaac agaagaaata ccatattgag
     3061 gatgtggact tcagcaaaac ggacattcat atccatgtcc ccgctggagc gacgcccaag
     3121 gagggaccgt ctgccggtgt cacgctggtc acggcgctgg tttcgcttct cacaggccgc
     3181 cgggcgcggg gtgacgtcgc catgacgggg gagatcacac tccggggaaa agtgatgccg
     3241 atcggaggcg tgaaggagaa aaccctcgca gcacagcggg cgggtattca caccgtgatt
     3301 ctgcccgcgc gcaacaagcg tgatctggag gacatcccga agacggtgcg gaagaaggtg
     3361 ac
//
LOCUS       DAOSOO010000292         3315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_28869_length_3315_cov_6.363497, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000292 DAOSOO010000000
VERSION     DAOSOO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28869_length_3315_cov_6.363497"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="PLT86_14005"
     CDS             <1..1986
                     /locus_tag="PLT86_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37770.1"
                     /translation="NSPYVVTGAITVATGVTLTIEAGVVVYFGGWYPFTVQGRMNANG
                     TAKDSVLFVQEPRSDTWLGLEFLGSDSSTLSYTRISNVEEDDTGGAVNVGGTARLRLT
                     HCVIAYNSGINGGAIFNGDGSESSIVYADWTVMHSNNAYGEGSAVYTSNTAKTYLTNC
                     TITKNTSGTSGAAVYSNAGTVTMLNCIVWNSGTSVTVAGGGTVTATYSDIQGTTVFTG
                     KGNINADPKFLAPARNIFLLQSSSPCVGAGAGGEVMGAFIFVPTTLTILQPSAMPGDT
                     LLLTIRGTFLPALAVDVAFRINTAAIMPDTVGKSLVSAHAFSSAADGAVWYNIVADTV
                     FLSFSATERVGLANQALVSLRFRVAPGAAGGDYPATFVASETNIEEFAVDNLNNGKVY
                     VQTLGDVTKTSGLTAADATEILKFVVRLRPTIDINLADVTCNGKVTSFDAAWVLQKVL
                     HPEYLFPCEGGGPLPRPAGHPALVLRWQREGDAWLLFGDHAGTLAGDLTLALGSEDAV
                     SATGSGTVVANQDGSVLHVGLVDFSGEPLLLRIEGAGASAQPLILAADINDGEYRISG
                     VRPVALALEQNVPNPFNPSTTIQFRVPDAGFVSVAIYGIDGQLVRSLVNRSVEAGPHE
                     VVWDGRDNAGRAVASGVYVYRLTSDRGVISRRMVLAR"
     gene            complement(2094..2411)
                     /locus_tag="PLT86_14010"
     CDS             complement(2094..2411)
                     /locus_tag="PLT86_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37771.1"
                     /translation="MPIETVIKLHQTGVWLVVASAITLIVATTVAVRLVPSFVLPLLV
                     VAWVCGLAGFAIVCYVQPSAVNILLAVLFLASAGFHGWRIVKRIRAAASSSCNDPTHH
                     HSS"
     gene            complement(2448..>3315)
                     /locus_tag="PLT86_14015"
     CDS             complement(2448..>3315)
                     /locus_tag="PLT86_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT37772.1"
                     /translation="PGSTILLGPLNDDQPLDKIEAVYGGVRKEMLPKLPNLRWVQTTS
                     AGVEGILYPEMVESDIVITNCKGIFSIFIAELAVAHLLALTSQVLAAALRPDRKTWLY
                     KPLRATELYGGTALIVGMGGIGSEIAERIQAFHMKIIGIDMIRKAGAGITLLRPDRLD
                     EVLPDADVVFVTAPATTAQKLFDRDRLFRMKKGAYLINSSRGKVVDTDALTEALQCGH
                     LAGAGLDALDPEPLPPEHPLWDMMNVSITPHIGGNTDALDRRRLLFLKENLWRYVRGV
                     PLLNVVNKRRGF"
BASE COUNT          660 a          993 c         1027 g          635 t
ORIGIN      
        1 aactccccgt acgtggtgac aggggcgatc acggtcgcca ccggcgtgac cctgacaatc
       61 gaagccggcg tggtcgtgta tttcggtggg tggtacccgt tcacggttca agggagaatg
      121 aacgcaaacg gcaccgccaa ggattccgtt ttgtttgttc aggaaccgag gtctgacacg
      181 tggctgggac tggaattcct gggcagcgac tccagcaccc tgtcctacac caggataagc
      241 aacgtggagg aggatgacac cggcggcgcc gtgaacgtag gcggcactgc gcgcctgcgg
      301 ctcacccact gcgtcatcgc ctataactcc ggaattaacg gcggcgccat tttcaacggg
      361 gacggcagcg aatcctcgat cgtgtacgcc gactggaccg taatgcacag caataacgct
      421 tacggggagg ggagtgcggt ttacacaagc aacactgcga aaacctacct caccaactgc
      481 accatcacga aaaacacttc agggaccagc ggcgcggcgg tatactccaa cgcaggcaca
      541 gttacgatgt taaactgcat cgtgtggaac agcgggacat ctgtaaccgt tgccggaggc
      601 ggaacggtaa cagccacgta ttcggacatc cagggcacga cagtattcac tgggaaaggg
      661 aacataaacg cggacccgaa attcctggct cccgcgcgaa acatcttctt gcttcagtcc
      721 tcctctccgt gcgttggggc aggtgccggc ggcgaggtaa tgggggcatt catattcgtc
      781 ccaactaccc tgaccatcct gcagccttcc gctatgccgg gcgacacact cctcttgacg
      841 atccgcggca cctttcttcc cgcgcttgca gtggacgtcg cgtttcgtat caacactgcc
      901 gccatcatgc ccgacaccgt ggggaaatcg ctcgtctctg cccacgcgtt ttcatctgcg
      961 gcggacgggg ctgtctggta caatatcgtc gctgacacgg tattcctgtc gttcagcgcg
     1021 actgaacgcg tcgggctcgc caaccaggca cttgtgagcc ttcgattccg cgttgccccc
     1081 ggtgccgcgg gtggcgatta ccccgcaaca ttcgtcgcgt cggagaccaa cattgaagaa
     1141 ttcgccgtgg acaatctcaa caacggcaag gtgtacgtgc agacgctcgg cgacgtgacg
     1201 aagaccagcg gccttaccgc cgccgacgca acggagattc ttaagttcgt ggtacgcctc
     1261 aggccgacga ttgacatcaa tctggcggat gtcacgtgca acgggaaggt cacgagtttc
     1321 gacgccgcat gggtactgca gaaggtgctg catccggagt atctcttccc gtgcgaagga
     1381 ggaggccctc ttccccgtcc ggcgggccac cctgcgctgg tcctgcgctg gcagagggaa
     1441 ggcgatgcgt ggcttctctt cggcgaccat gccggaacac ttgcgggtga cctgacgctg
     1501 gcgctgggtt cggaggatgc agtcagcgca acgggcagcg gcaccgtggt cgccaatcag
     1561 gacggcagcg ttctgcacgt cgggctggtt gatttctccg gcgagccgtt gctactgcgc
     1621 atagaaggag cgggcgcatc ggcacaaccg ttgatccttg ccgctgacat caacgatggc
     1681 gagtacagaa tttccggcgt ccgtccggtg gcgctggcgc tcgagcagaa cgtaccgaac
     1741 ccgttcaacc cctccaccac gatccagttc cgcgttccgg atgcggggtt cgtcagtgtg
     1801 gcgatttacg gcattgacgg gcagcttgtc cgttcactgg tcaaccggtc cgtagaagcg
     1861 ggtccgcacg aggtcgtgtg ggatggacgc gacaacgcag gccgggctgt ggcgagcgga
     1921 gtgtacgtgt atcgcttgac ttcggatcgg ggtgtgatat caaggagaat ggtgctggcg
     1981 cggtaggaaa ccacctgcac cgcgggacga atttcgcggc atcaacgggg gcgactgcaa
     2041 cgtcgccccc gttttttcgt gctgaggaga ggctctcggc ccgcgaaggg ctttcagctc
     2101 gagtggtgat gcgttggatc gttgcaggaa gaggaagccg ctgctcggat ccgtttcacg
     2161 attcgccaac cgtgaaaacc tgcgctggcg agaaacagca cggcaagcag gatattgacc
     2221 gcgctcggct gcacgtaaca gacaatcgcg aaccccgcca gcccgcagac ccacgccact
     2281 accagcaggg gaagcacgaa gctgggaacc agccgcaccg ccacggtggt cgccacgatg
     2341 agcgtaattg cggacgcaac aaccagccag acgccggtct ggtggagctt tatcacggtt
     2401 tcgatgggca tgctgacctc ctcatgtgcg gggtgaacag cgacgcatca gaacccgcgc
     2461 cgcttgttga caacgttcag cagaggcacg ccacggacgt aacgccagag gttctctttg
     2521 agaaacagga gccgacgccg gtcgagcgcg tccgtgttgc cgccgatgtg cggggtgatg
     2581 gatacgttca tcatgtccca gagcggatgt tccggcggca gcggttcggg gtcgagggcg
     2641 tccagaccgg ctcccgcaag gtggccgcat tgcagagcct cggtcagcgc atcggtatcc
     2701 accaccttgc cgcgggaaga attgatcagg taggcacctt ttttcatgcg gaagaggcgg
     2761 tcccggtcga aaagcttctg ggccgtcgtc gccggtgctg taacaaacac gacatccgca
     2821 tccggcagta cctcgtccag ccggtcaggc ctcaacaggg tgattccggc tcctgctttt
     2881 cgaatcatgt cgatgccgat gatcttcatg tgaaacgcct ggatccgctc ggcgatttcc
     2941 gacccgatgc ctcccatgcc cacgatcagt gccgttccgc cgtaaagctc cgtcgcccgg
     3001 agaggcttgt agagccacgt ttttcgatcg ggccgcaacg cggcagccag tacctgtgac
     3061 gtgagggcaa gaaggtgggc aacggcaagc tcggcgatga agatgctgaa aatgcccttg
     3121 caattggtga tgacgatgtc gctctccacc atttcggggt agagaatacc ctccacaccc
     3181 gcgctggttg tctgcaccca ccggaggttc ggcagctttg gaagcatttc cttccgtacg
     3241 ccgccgtata cggcctcgat tttgtccaga ggctggtcgt cgttcagagg tccgagcaga
     3301 atcgtggacc cgggc
//
LOCUS       DAOSOO010000293         3299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29061_length_3299_cov_5.290382, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000293 DAOSOO010000000
VERSION     DAOSOO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29061_length_3299_cov_5.290382"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLT86_14020"
     CDS             complement(<1..165)
                     /locus_tag="PLT86_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37773.1"
                     /translation="MKITAIDHFVCQAGGRNWVFVKVSTDEGVYGVGEGSLYGKEHTM
                     RSAIEELRRDI"
     gene            complement(162..1352)
                     /locus_tag="PLT86_14025"
     CDS             complement(162..1352)
                     /locus_tag="PLT86_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37774.1"
                     /translation="MKVQIPFPISFWCGVPADFVAPERYKEIAEAGFTVAQVSGTREE
                     IVRQLNWCEENNITGMVLDRRVHAGMTGASDWRDTVREVVLDYSWHPALWGYYLTDEP
                     SASQFGTLAEITREFEKLDPAHPTYINLFPNYANEEQLGTATYEEHIRQYLDIVQPPL
                     VSYDHYAIQQDGNDWPIFYTNLSVVREQSLEAGKDFWQIVLSLAFHNCRNPSEADLRW
                     QVWMTLAYGGKGISYFTYWTVTPENFRDAIIDEFGYQTSHYPMVRRINRQIQNIGAAL
                     LRLKSLGVWHAPVPFKGVVPKEDYAPQFVVGSSDVPISVGEFTDGDVPALIVVNRSLE
                     RSVPAEVRVKPEFAAYREANRTSGGVSGPMKLSRSDAFASFHVWLAPGDGALLYLDRE
                     GITS"
     gene            complement(1363..2106)
                     /locus_tag="PLT86_14030"
     CDS             complement(1363..2106)
                     /locus_tag="PLT86_14030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT37775.1"
                     /translation="MPAEFRSPDWDSYRMDMHDPHRELEPLEREVRIVDQALERLRAA
                     GIIPHTRYDSDKMLAFRAQVAASFDIPWTAITPRQQRLIWAINAICQPKTMVAAGVFC
                     GNTFISNAGAAVGAGAAYRAERLIGLEIKQDEAARAERNVRRIDPTGVAKCVAADAVD
                     FVRDMTGEINLLYLDADGAGGRGKGVYLDILEAAWDKMPKGSIVLAHNSVNCAEKLSY
                     YLAFVRDTANMEASMNVVIDGEGLEVSAK"
     gene            complement(2096..3202)
                     /locus_tag="PLT86_14035"
     CDS             complement(2096..3202)
                     /locus_tag="PLT86_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37776.1"
                     /translation="MIPYEGKNPRTVNFLKTLYFDTPQWVPCGVSLMMATWMKYREEL
                     EAVVLAHPRIFPGYVKGSINLDFPDAYVLYEPGYHTDNWGCVWHNIERGLDSIVVKHA
                     LENWDDFSNWKAPDPLTQADFGGRPSLEQIKSGLAAARARGDLAGGGGLAHGFFYMRL
                     FYLRGFENLMMDFANDDPRLHRLIQIIEGYNVAVIQQYLEAGAEYMSFGEDLGTQKSL
                     PISPAMWRKFIKPSYEAMFGPCRDRDIPVYLHSDGHILEIIPDLIDVGIRMINPQIRA
                     NTLEGIRDVMKGRIAIALDLDRQLFPFASFSEIEDHIAGAYDALNMKEGGLTMGAECE
                     PDVPLERIDWICSVLERVCKPPLPEELGEKSRAS"
BASE COUNT          692 a          983 c          896 g          728 t
ORIGIN      
        1 gatgtccctc cggagttcct cgattgcgga acgcatggtg tgctctttgc cgtacagcga
       61 gccttcaccc accccgtaca caccttcatc cgtggagacc ttcacgaaaa cccagttccg
      121 accgcctgcc tggcatacga agtggtcaat tgccgtaatc ttcacgatgt aatcccttct
      181 cgatcgaggt agagcaacgc gccgtctccg ggggcgagcc agacgtggaa tgaagcgaag
      241 gcgtcacttc gcgacagctt catggggccg gatacaccgc cgctggtgcg gttcgcttcg
      301 cggtacgcgg cgaattccgg tttgaccctt acctccgctg gcacactccg ttccagggag
      361 cggttcacca ctatgagcgc cggaacgtct ccgtccgtga actcgcccac agaaatcggc
      421 acatcgctgg aaccgaccac gaattgcggc gcatagtctt ctttcggtac aacgcccttg
      481 aaaggcactg gcgcatgcca caccccaagg gacttcaggc gcaagagggc tgcaccaatg
      541 ttctgaatct ggcggttgat ccgccgaacc atcgggtaat gcgacgtctg gtatccgaac
      601 tcgtcgatga tcgcgtcccg gaaattctca ggagtaacgg tccagtaggt gaaataactg
      661 atccctttgc ccccgtaggc gagcgtcatc cacacctgcc accggaggtc tgcttccgaa
      721 ggattccggc agttgtgaaa cgcgagcgat aacacaattt gccagaagtc ttttcccgct
      781 tcaagtgact gctcacgaac aaccgagaga ttggtgtaaa agatgggcca gtcattgcca
      841 tcttgctgga tggcgtaatg gtcgtagctc accagtggcg gctggacgat gtccaggtac
      901 tgccggatat gttcctcgta cgtcgccgtg cccagttgct cttcgttcgc gtagttcgga
      961 aaaaggttga tgtacgtggg gtgcgcggga tccagttttt cgaattcccg cgtgatttcg
     1021 gcgagtgttc caaactggga agcagagggt tcatctgtca ggtaataccc ccagagtgcc
     1081 gggtgccagg agtagtcgag gactacctcc ctcaccgtgt cacgccagtc ggaagcgccc
     1141 gtcatgcccg cgtggacacg gcgatccagc accattcccg tgatgttgtt ctcttcacac
     1201 cagttgagct gccgaacgat ttcttcgcgt gttccagaaa cctgggcaac agtgaagccg
     1261 gcttcggcga tttctttgta tctctccgga gccacgaagt cagcgggcac tccgcaccag
     1321 aagctgatag gaaatggaat ctgaactttc atcgcgttcc cctcacttgg cagatacctc
     1381 aagcccttcg ccgtctatca caacattcat gcttgcctcc atattggcgg tatcacgcac
     1441 gaacgccaga tagtacgaaa gtttctcggc gcagttgacg ctgttatgcg cgagaacgat
     1501 ggatcccttc ggcattttgt cccacgcggc ctcaaggatg tcgagataca ctcccttacc
     1561 acgaccgccg gcaccgtctg catcgaggta caaaaggttg atttctccgg tcatgtctcg
     1621 cacgaaatca acggcgtcag cagccacaca tttcgccacg ccggtaggat ctatgcggcg
     1681 cacatttcgt tctgcgcgcg ccgcctcgtc ctgttttatc tccaacccga tgagtcgttc
     1741 ggcacggtag gcagcgcccg ctcccacggc ggcgccggcg ttggaaatga acgtgtttcc
     1801 acaaaaaaca cccgccgcca ccatcgtctt cggttggcag atggcattga ttgcccatat
     1861 aagccgttgt tggcgaggag tgatggctgt ccacgggata tcaaagctgg ctgccacctg
     1921 cgcccggaat gcaagcatct tgtcagaatc gtaccgggtg tgtgggataa ttcccgctgc
     1981 gcgaagacgc tccagagcct ggtccacgat ccggacttcg cgttccaacg gctccagctc
     2041 gcggtgcgga tcatgcatgt ccatgcggta agagtcccaa tcgggactgc gaaattcagc
     2101 tggcacggga tttctctccc agttcctcag gcaatggcgg cttgcagaca cgttccagaa
     2161 cggaacagat ccagtcaatt cgttcgagcg gcacatccgg ttcacattcc gcacccattg
     2221 tgaggccgcc ttctttcatg ttgagtgcgt cgtatgcgcc ggcaatatga tcctcaatct
     2281 ccgaaaagga cgcgaaaggg aacagctgcc tgtcgaggtc cagcgctatt gcgatgcgac
     2341 ctttcatgac gtctctaatc ccttccaggg tgttggccct tatctgggga ttgatcatcc
     2401 ggattccaac gtcaatgagg tcgggaatga tctcgaggat atggccgtcg gaatggagat
     2461 acaccggaat gtcgcggtct cggcacgggc cgaacatggc ttcgtacgag ggcttgatga
     2521 atttccgcca catcgccggg ctgatcggga gcgatttttg agtgccaaga tcctcgccga
     2581 acgacatgta ctcggcaccg gcttccagat actgctgaat tacggccaca ttgtagccct
     2641 caatgatctg gatcagcctg tgaaggcgcg ggtcgtcgtt ggcaaaatcc atcatgaggt
     2701 tttcgaaccc gcggagatag aaaaggcgca tgtaaaagaa cccgtgcgcc aatcctccac
     2761 cccccgcgag atcaccgcga gcgcgggcgg cagccaagcc acttttgatc tgttccagag
     2821 aaggcctccc gccaaaatcg gcctgcgtca gcggatccgg agccttccag ttggaaaagt
     2881 catcccagtt ttcaagggca tgtttcacca caatgctgtc gagtccccgc tcgatgttat
     2941 gccagacaca cccccagtta tccgtgtggt accccggttc gtacaacacg tacgcgtcgg
     3001 ggaagtcgag gttgatgctc cctttcacgt aacccgggaa tatcctcggg tgcgcgagaa
     3061 caacggcctc gagttcttcc ctgtatttca tccacgtcgc catcatcagg ctcaccccgc
     3121 acggaaccca ctgcggcgta tcgaagtaca gggttttcaa aaagttgact gtgcgggggt
     3181 ttttgccctc ataaggaatc atccgaagtc tcctgttgct gctgccctag aaaagcgaga
     3241 gtccatcaac gctgacgtgc catcgcatcc cacgtgcgct ccagatgctg gcggatcgg
//
LOCUS       DAOSOO010000294         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29072_length_3298_cov_3.997533, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000294 DAOSOO010000000
VERSION     DAOSOO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29072_length_3298_cov_3.997533"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLT86_14040"
     CDS             complement(<1..253)
                     /locus_tag="PLT86_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37777.1"
                     /translation="MSSSPERKRDLALNLGASTGVDHYVIVSVTAPREFLDVLTDCAV
                     ELGTLGSYESGDDPVNLTFYFRPPLHEEEARSAFLESVPS"
     gene            532..2235
                     /gene="argS"
                     /locus_tag="PLT86_14045"
     CDS             532..2235
                     /gene="argS"
                     /locus_tag="PLT86_14045"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT37778.1"
                     /translation="MTRVFAPLISLFAEATELEEREVAGLLCPPPSAELGDFAFPCFV
                     LAKRLRKSPAQIAAELAERVNSNDTIASAVAVGPYVNVRLHRAPFISTLLEEVRNQGE
                     QFGSSSSGNGKCVVLDFSSPNMAKPFHVGHLSSTIIGGSLSRLFAYQGYRVVRINHLG
                     DWGTPVGQQITAFKRWGDGTELDRHPIAHMVDLYQRFHREKAENPGLEEEAREWFRKL
                     ETGDKEAREFWEHIRSVSIQELNRAYARLNVEFDAYLGEAFYEGMLQETIEEVQKAGI
                     TEVSDGALIVPLDSKGIKTPLILRKSDGSTIYATRDIAAARYRAENYRFDLCLYVVAH
                     DQELHFRQLFATLELMGYDWAKRCVHVQFGHVHGMSTRKGGAILLDELLDRATEKVRE
                     TVMANQTLFRDVPAAEIDEVCEAVGVGAVIFGTLNHRRTKDVEFSWDNALNFNGETGP
                     YVQYAHARLCSILRKAGDEPRGAANVELLTHDAEWALVKELARFPEVVEEACAQYEPS
                     CVARYAVDLARAFTVFYDQCRVLGEEPELSAARLSLVNTTRVVISSALHLLCMKTPER
                     M"
     gene            2235..2945
                     /locus_tag="PLT86_14050"
     CDS             2235..2945
                     /locus_tag="PLT86_14050"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOT37779.1"
                     /translation="MTMSTLTLPELPVYVCRRTARPISVDGILDEPDWGAAEPMRLVR
                     TESGAVPEQPTCVRVLWDDEYLYVAFECVDTDVWATIEERDGALWDEEVVEVFVDDDS
                     DSRTYLEYEINPLNALVDLYVLNRTGRRDAIKFMLDWNSSGIRHAVRVDGDPRRRGSS
                     DRGWTAEWAIPFGDFTTAPNIPPRNGDAWRINFYRIDRGDRDEYSAWSPTGAINFHVP
                     ERFGTLVFSTDTVTRKDH"
     gene            2962..>3298
                     /locus_tag="PLT86_14055"
     CDS             2962..>3298
                     /locus_tag="PLT86_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOT37780.1"
                     /translation="MNDTPLVLCRRTAEQITVDGVLSERSWAAVEPVTLCRTDTGDAA
                     RFRTEVRLLWDDTYLYASFHCVDTDIWANMVLRDAPLWEEEVVEMFLDPNRSGNAYFE
                     FVVNPLNTLT"
BASE COUNT          699 a          883 c         1033 g          683 t
ORIGIN      
        1 gggacggtac cgactcgaga aaggcggagc gagcctcttc ttcgtggagt ggcggacgga
       61 agtagaacgt gagattgacc gggtcgtccc cggattcata tgaaccgaga gttccgagct
      121 ccacggcgca atccgtcaag acatcaagga actcacgcgg cgcggttacc gaaacgatga
      181 cgtaatgatc cactccagtc gatgccccca gattcagtgc cagatcgcgc tttcgttcag
      241 gcgacgaaga caatccctgc cgtgaaccgt tctcgaatgt cgcttttgtc gcacccgtgc
      301 cgacggaacc ctctcaaaag accatgcaag aagcaaagct cctccctcag tgtcacatta
      361 acaaggtaat tgacgggcat ccatgttgga atccgtcgtc ggcggcgatt ggccgaggga
      421 ggtggcggtc agtatcctta ttcagggcca atgcatcggg ggaaggccgg tttatccggg
      481 ccggaattcc ctcgtcgatg aaaaaagaaa ggtgatcgag agagcgaacc tatgacgaga
      541 gtattcgcgc cattgataag tctttttgcg gaggcaaccg aactggaaga gcgcgaggtc
      601 gcgggccttc tgtgcccgcc tccgagtgcc gagctcgggg atttcgcgtt cccgtgtttt
      661 gtgcttgcga agcgtctgcg aaaatcgccc gctcaaattg ccgcagaact cgcagaacgt
      721 gtgaattcaa acgacaccat cgcttcggcc gttgcggtgg ggccctacgt gaacgttcgg
      781 ctccatcgag caccgttcat cagtacgttg ctggaggagg tacgcaacca gggagagcag
      841 tttggctctt cttcgagcgg caacgggaaa tgcgtggtcc tggacttctc atccccgaac
      901 atggcgaaac cgtttcacgt cgggcatctc agcagcacga taatcggcgg ctctctttcc
      961 cgtttgtttg cgtatcaggg ataccgcgtg gtgcgcatca atcatctcgg tgactggggc
     1021 acgccggtgg ggcagcagat tactgcgttc aagcgttggg gggacggaac ggagcttgac
     1081 agacacccca ttgcccatat ggtggacctc taccagcggt ttcacaggga aaaagccgag
     1141 aaccccgggc tggaggaaga ggcgcgcgag tggtttcgaa agctggaaac gggtgacaaa
     1201 gaagcccggg agttctggga gcacattcgt tccgtttcga ttcaggagtt gaaccgcgcc
     1261 tatgcgcggc tgaatgttga gttcgatgcc taccttggcg aggcgttcta cgaggggatg
     1321 ctgcaagaga ccattgagga ggtacagaag gcaggaatca cggaggtttc cgatggcgct
     1381 ctcatcgtgc cgctcgattc gaaagggatc aagactcctc tcattctcag gaagagtgat
     1441 ggctcaacga tttatgcaac ccgcgacatc gcggcggcgc ggtaccgtgc ggaaaactat
     1501 cgtttcgacc tttgccttta cgtggttgct cacgaccagg agcttcattt caggcagttg
     1561 tttgccaccc tcgaactgat ggggtacgac tgggcgaaga ggtgcgttca cgtgcagttc
     1621 ggccacgtgc acgggatgtc cacgcgcaaa ggtggcgcca tcctgctgga cgagttgctc
     1681 gaccgtgcaa cagagaaagt gcgcgaaact gtgatggcga accagaccct ttttcgcgat
     1741 gttcctgcgg cggaaataga cgaggtgtgc gaggcggtgg gtgtcggagc cgtaattttc
     1801 gggaccctga accatcgtcg cacgaaggac gtggagttct cgtgggataa cgcactgaat
     1861 ttcaacggcg aaacgggacc gtatgtgcaa tatgcgcatg cacgtctctg cagcatcctg
     1921 agaaaggccg gggacgagcc acgcggggca gcgaacgtcg agctcttgac gcacgatgcg
     1981 gagtgggcgc ttgtgaagga gctcgcgcgg ttccctgagg tggtggagga agcgtgtgcc
     2041 cagtatgagc cttcctgcgt cgctcgctac gccgtagacc ttgcccgcgc gttcacggtg
     2101 ttctacgatc agtgccgcgt tctgggggag gaacccgagc tctccgcagc gcgcctttcg
     2161 ctggtaaata ccactcgcgt cgtgatctcc agtgcgcttc atctgctgtg catgaaaaca
     2221 cctgaacgga tgtgatgact atgtccaccc tgacgctgcc tgagctcccc gtatacgtgt
     2281 gccgtcgaac ggcacggccc atctccgtag acggaatcct cgatgaaccc gactggggag
     2341 cggcggagcc gatgcgcctc gtccggaccg aatcaggggc cgtgccggaa cagccgacgt
     2401 gtgtccgggt tctctgggac gacgaatacc tttacgtagc ctttgagtgt gttgataccg
     2461 atgtgtgggc gacgattgaa gagcgcgatg gtgctttgtg ggatgaggaa gtggtggaag
     2521 tgtttgtcga tgacgacagc gattccagga cttatcttga atacgaaatc aacccgttga
     2581 atgcgctcgt tgatctctat gttctcaaca ggactggtcg acgggacgcg atcaagttca
     2641 tgctggactg gaactcttcc ggcattcgcc atgccgtgcg ggtggacggc gacccgcgga
     2701 gaaggggaag cagcgatcga ggatggacgg ccgagtgggc catcccgttt ggcgatttca
     2761 ccacggcgcc gaacattccg ccgcggaacg gggatgcgtg gcgcatcaat ttctaccgca
     2821 tagaccgagg cgaccgggac gagtattccg cgtggtcacc caccggcgcg atcaatttcc
     2881 acgtgccgga acggttcggg acccttgtct tctcaacgga tacggtgacg cgcaaggatc
     2941 actgaaatgg aggagcgacc gatgaacgat acacctctcg tattgtgtcg gcggacggct
     3001 gaacagatca ccgtggatgg agtcctgtca gaacgttcct gggctgcagt ggaaccggta
     3061 acgctctgcc gaacagacac cggagatgca gccagattcc gaaccgaggt ccgcctcctc
     3121 tgggatgaca cttacctcta cgccagtttc cactgcgtgg ataccgacat ctgggcgaac
     3181 atggtcctgc gggacgcgcc actctgggag gaggaggtcg tagagatgtt cctcgatccg
     3241 aatcggagtg gaaacgcgta ttttgaattc gtagtaaacc ctttgaacac tttgactg
//
LOCUS       DAOSOO010000295         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29183_length_3290_cov_4.853478, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000295 DAOSOO010000000
VERSION     DAOSOO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29183_length_3290_cov_4.853478"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLT86_14060"
     CDS             <1..523
                     /locus_tag="PLT86_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT37781.1"
                     /translation="EFGQVDVLMNNAGIVINTSAEEMTDAQWEDVLAVDLTGVFKCCR
                     AVAPHMIARKRGSIINIGSMSGVVSNHPQPQCSYNAAKAGVIMLSKSLAGEWAQYGIR
                     VNTISPGYMETEMTLRGRQNPRENDVWIENTPMKRCGRPEELGPLALYLASDASSFMT
                     GANILIDGGYTVW"
     gene            complement(526..1587)
                     /locus_tag="PLT86_14065"
     CDS             complement(526..1587)
                     /locus_tag="PLT86_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOT37782.1"
                     /translation="MIPYSLTQPQPFRAKRLSSYDRAGGNNDWIPIGGRSTCVIGDIR
                     GPGIISHLYFTVNSPDPHYLRSLVLRCFWDDEPFPSVECPLGDFFCVGHGAVSTFENA
                     AFSMAANKSKTYGRFAPMNCWVPMPFSERARLEITNDPDIPVIAFYFHIDYQEYEQLP
                     GTPWYLHATWRRENPCDGWKGTGSVVWGPKWAARHQTEDDSNLTGSGNYVILDTKGRG
                     TYLGCNLSVDNLYKGWWGEGDDMIFVDGEVWPPSLHGTGTEEYFGQGWGMQDVQHQYY
                     GLSYHEREDFNDRGKVTVYRFHVADPIPFRESIRVTLEHGHANDRSDDYASTAYWYQA
                     EPHVPFAALPDVGARLPNA"
     gene            1847..3076
                     /locus_tag="PLT86_14070"
     CDS             1847..3076
                     /locus_tag="PLT86_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT37783.1"
                     /translation="MFRGTRSVLSVGAAGVAAVLLAAAIPSPAADGGALAPSWDKGLV
                     LQTADKEFRIRVGGRIHQDWTFFTSQSDSLTRRFGDLQDGTEFRRARIHVDGIAYGTT
                     EWRAEFDFGGGDASFREVYIGLRGVPGLSTLRLGHMQEPFSLEEITSSNYLVFHEYGL
                     AGVFTPAYNAGLLLSGTFYSGRAGWAAGVFRDSDNYGKNSGDGKYAFSGRVVFAPAYS
                     KEQKRVIHLGFSGSTRNPNGGVVKYSRKPEVNLAPNFLNTPTIAAERVNLFGVETLVS
                     QGRFFAQAEGIGSRVTRTGGGSATFTGGYAYAGWFLTNDHRPYKPGQGVADRVVPTRN
                     FDPRKSEWGALAVAARVSYLDLDDSGIKGGTLTDGTLGLDWFLNPNTSVKAGYVIARP
                     KGAGAMSAFQLRAQFDF"
BASE COUNT          685 a          935 c          990 g          680 t
ORIGIN      
        1 cgaattcggg caggtggacg ttctcatgaa caacgccgga atcgtcatca acacgtccgc
       61 ggaggagatg acggacgcgc aatgggaaga cgtgctggcc gtggatctga cgggggtgtt
      121 caaatgttgc cgtgccgtgg cgccacacat gattgcgcgg aaacggggtt ccatcatcaa
      181 catcgggtcc atgtccggtg tggtgtcgaa tcatccacag ccgcagtgct cgtacaacgc
      241 cgccaaagcc ggtgtcatca tgttgtcaaa gtcgttggcg ggtgaatggg cgcagtacgg
      301 tattcgtgtc aacacgatct cccctgggta catggaaacc gaaatgacct tgaggggaag
      361 gcagaatcct cgagagaacg acgtctggat cgagaacacg ccgatgaaac gttgcggcag
      421 gcctgaggaa ctcgggcccc tcgcactcta ccttgcttcg gacgcctcca gtttcatgac
      481 gggcgcgaac attctcatcg acggtgggta caccgtctgg tgaaatcacg cgttcggcag
      541 gcgagcaccc acatctggca gagcggcgaa cggcacgtgc ggttcggcct ggtaccagta
      601 cgcggttgag gcatagtcgt ccgaacgatc attggcatgc ccgtgctcca gtgttacccg
      661 gatcgactcg cggaaaggta tcggatcggc aacgtgaaac cggtagacgg ttactttgcc
      721 gcggtcgttg aagtcctcac gctcgtgata cgatagcccg tagtactggt gctggacgtc
      781 ctgcatcccc cagccctgtc cgaagtattc ctccgttccg gtgccgtgca aggaaggcgg
      841 ccacacctcc ccgtcgacga aaatcatgtc gtcgccctcg ccccaccacc ctttgtacag
      901 attgtccacc gacagattgc atccgagata cgttccgcgt cccttcgtgt cgaggatgac
      961 gtagttgcca ctgcctgtca ggttgctatc gtcttcggtt tggtgccgtg cagcccactt
     1021 gggcccccag actacggaac cggtcccttt ccatccgtcg caggggttct cacggcgcca
     1081 tgtcgcgtgc agataccatg gtgttcccgg caactgctcg tactcctggt aatcgatgtg
     1141 gaaatagaaa gcgataacag gtatatctgg gtcattcgtt atttcaaggc gcgcgcgttc
     1201 ggaaaaaggc atgggcaccc agcaattcat cggggcgaag cgtccgtatg ttttcgattt
     1261 gttcgcggcc atactgaagg cagcgttttc gaacgtgctg accgcgccgt gccccacaca
     1321 gaagaaatcg cccaacggac actcaacaga tgggaacggt tcgtcatccc agaagcatct
     1381 caagacgaga gaacggaggt aatgcggatc agggctgttc accgtaaaat acagatgtga
     1441 gatgattccg ggtccgcgga tgtctcctat gacgcacgta gaccggccgc cgatgggtat
     1501 ccagtcgttg ttccctccgg cgcggtcgta actcgacagg cgtttggcac gaaagggttg
     1561 aggctgggtt agtgaatacg gtatcacggt gcagggcccc ttttcgggtt ggatgggaac
     1621 acaaccggac cggtgcggat cgcacaccat gcgtcccacg agcggaaacg tccgcacccg
     1681 tgcgaaagtg cgatcgatgg ggtggttctc aaaattcgca aaaaactcac cggtacgaaa
     1741 cgttctctta atggactcgg gcactttctg tactgcgggt cggaggattc gccggtgaat
     1801 gacaaaatga tttcgccgta gtctcacccc tcaaaaagga gcacgtatgt ttcgcggaac
     1861 gcgttcggtt ctcagcgtag gtgcggcagg cgttgctgcc gttctgctgg ccgctgccat
     1921 cccctcccca gcggctgacg gtggagccct cgcgccctcg tgggataagg gactggtact
     1981 gcagacggcg gacaaggagt tccgtatccg tgtcggtgga cgcatccacc aggactggac
     2041 ctttttcact tcccaatctg actcgctgac caggcggttt ggtgacctgc aggatggtac
     2101 cgaattccgc cgcgcccgga ttcacgtcga tggaatcgcg tacggcacga ccgagtggcg
     2161 ggccgagttc gacttcggcg gcggcgacgc aagtttccga gaagtctaca tcggtctccg
     2221 cggcgtgccg gggctgtcaa cgcttcgtct gggccacatg caggagccat tcagcctcga
     2281 ggaaatcacg agcagcaatt acctggtatt tcacgaatat gggctggcag gcgtcttcac
     2341 accggcgtac aatgccggac ttcttcttag cggcacgttt tactcagggc gtgcgggctg
     2401 ggctgccggt gtcttccgtg attcggacaa ttacggcaaa aacagtggcg atgggaagta
     2461 cgccttctcg gggcgtgtcg tatttgcgcc ggcctattcg aaagaacaga aacgagtcat
     2521 tcacctcggg ttctcgggca gcaccagaaa tcccaacggg ggtgttgtga agtacagccg
     2581 gaaacccgaa gtcaatcttg cgccgaattt cctcaacacc ccaaccatcg cggcagagag
     2641 ggtcaatctg ttcggggtgg aaacactcgt ttcacagggg cgcttcttcg cgcaggcgga
     2701 agggatcggg agccgtgtga ccagaacagg tggcggcagc gcgacgttca ccggtggata
     2761 cgcctacgcg ggatggttct tgacgaacga tcacaggccg tacaaacccg gacagggtgt
     2821 cgccgatcgc gtagttccca cgcgcaattt cgacccgaga aaaagcgagt ggggtgcgtt
     2881 ggcagttgcg gcgcgcgtct cttatctcga cctggatgac tcgggtatca aaggcggaac
     2941 acttacggac ggcacactcg gtctcgactg gttcctcaat cccaacactt ccgtcaaagc
     3001 gggctacgtg attgcgcgcc cgaaaggagc cggcgccatg agcgcgttcc agctccgggc
     3061 acagttcgac ttttgacgcg cgtctccggc gagtttccgg tagacacgaa tctggtttcc
     3121 gtggcggtca aatacccgct ctacgcatac ttctaacgtt cgaacaaagg aaacacgatg
     3181 attacgtcaa acaggctgat gttggcgtcc tgcgcgttca tcgctctggc gctgaccgga
     3241 agaccctcat acgcgtccgc cggatcgaga acgtcgttga cgatcaaagg
//
LOCUS       DAOSOO010000296         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29209_length_3288_cov_4.975255, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000296 DAOSOO010000000
VERSION     DAOSOO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29209_length_3288_cov_4.975255"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PLT86_14075"
     CDS             <1..1339
                     /locus_tag="PLT86_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT37784.1"
                     /translation="VLSLSLPPARNAERRDIGARRKREEEVRALKAKTAEIANKASLG
                     ALPESVLIPAPEILGLLVRSARERGIVLSINGFPRGASLDLDTSGLNKVVLSDNASRV
                     VVAEAGITLNDLASHLAANGLWFPGSPYVDGGIRLGDFLAWETEGAYARGYGRMYTRV
                     LGLEAVTGRGDVMSWGGMVRMQHAGLRLSELCLGKRNRYALITAVALRVAVIPATKQC
                     VVASYDNVASASSFARLALRGGQGSSGHLAPRAVALFNGPPGGGRSGKGVSVVVEFSG
                     TSSSVAGHVNRATVLAAVEGADHITSHEDQACEGLWRRISERYSPVPNSADDGNLKLV
                     LTTPPDTLGFCLDHALHVCSRRGRRCEFVADALAARADIVIPNGAAVAAEILRELDEN
                     LVETPARIEVFSLHEGLQEFLQNQRQTQLAEELKQQFDPSGILPSGLLPAIGR"
     gene            1356..1808
                     /locus_tag="PLT86_14080"
     CDS             1356..1808
                     /locus_tag="PLT86_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37785.1"
                     /translation="MSLLIDPKALRKWASNDLDVQKNQDGSYDFLFRYEGSSCRNGGR
                     AFPAEIRVCLSPADDGDWRIKDFAIIVPPAGREGWEATCIFGEIGKDSVRRMGTSVPF
                     RGQSLDEVLASDVALNHAGCFCSPAHVNHKALLALWTIKWWLDDTAKM"
     gene            1915..>3288
                     /locus_tag="PLT86_14085"
     CDS             1915..>3288
                     /locus_tag="PLT86_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOT37786.1"
                     /translation="MYTIVISQKNPEMDAHARGTLADVRDHGLTGITRVEVDRIFRVE
                     GELSREAAELLANRLFVDPVVETWAVYGAGETPDRRGANHVLEVAYNPGVMDPVEGSV
                     LKASGDLGVALRAVRTAARYRFYGDATASEILEAAYALLANKTVNHVVMGNDDLPKPF
                     PAEEFRGPSHVELVGCDDEGLMGISRGRGLSLSVSEMRRIKEYFEHLGRNPTDIELET
                     LAQTWSEHCVHKTFKGRIEFDGEVIDNLLKQTIVKATQELAKPWCVSVFDDNAGVIEF
                     NEKNHICFKVETHNHPSALEPYGGAGTGIGGVIRDVLGTGLGAKPVANTDVFCFARPD
                     YPADEIPEGILHPRRIMRGVVAGVRDYGNRMGVPTVNGAVIFDDRYLGNPLVYCGTVG
                     LLPVGCERKTALPGMKIVVVGGRTGRDGIHGATFSSAELGSDEENVWSNAVQIGNPIT
                     EKKVTDVL"
BASE COUNT          695 a          878 c         1057 g          658 t
ORIGIN      
        1 cgttctgtct ctttctctcc cgccggcgcg aaacgcggaa cggcgtgaca ttggtgcacg
       61 caggaagcgt gaggaagagg tcagagcgct gaaggccaaa acggcggaga tcgcgaacaa
      121 ggcatcgctc ggcgctcttc ccgagtcagt cctcatcccg gccccggaga tactggggtt
      181 actggtgagg tcagcgaggg aacgaggtat cgtgctgtca ataaacggct ttcctcgcgg
      241 cgcttccctc gacctcgaca cttcgggttt gaataaggtc gtgctttccg acaacgcaag
      301 cagagttgtt gtcgcggagg cagggattac tctgaacgac ctagccagtc atctcgctgc
      361 caacggactg tggttcccgg gatcgcccta cgttgacggc ggcatccgcc tcggggattt
      421 tcttgcctgg gagactgaag gcgcgtatgc gcgagggtac gggagaatgt atacaagggt
      481 cctcgggctg gaggcggtta cgggccgcgg agacgtgatg tcctggggcg gaatggtccg
      541 tatgcaacat gccggattgc gactctctga actctgcctc gggaagcgca accggtacgc
      601 gctcatcact gcagttgcgc tgcgagtcgc ggtcattccg gccaccaaac agtgcgtggt
      661 cgcctcttac gacaatgtcg cgtccgcgtc gagcttcgct cgcctcgcgc tgcggggagg
      721 gcaggggagt tcaggacacc ttgccccccg cgcagtagca cttttcaatg gtccgccggg
      781 aggtggtcgc tcgggaaaag gcgtctctgt ggtggtggag ttttcaggga cctcctcttc
      841 ggtggcggga cacgtgaacc gtgcgacggt actggcagcc gtggaggggg cggaccacat
      901 cacgtcgcac gaagatcagg cgtgtgaagg cctctggagg cgcatctcag aacggtacag
      961 ccccgtgccg aactccgcgg acgacgggaa tctgaagctg gttctgacta ccccgccgga
     1021 cacgctcggg ttctgccttg accacgcgtt gcatgtttgt tcacgccgcg ggcgcagatg
     1081 tgagtttgtt gccgatgcgc tggctgcgcg agcagacata gttattccga acggggcggc
     1141 ggtggctgcc gaaatactgc gggaactgga cgagaatctg gtggagacgc ccgcaagaat
     1201 cgaggtgttt tcgttgcatg agggcctgca ggaattcctg cagaaccagc gacaaactca
     1261 gctggcggag gaactcaaac agcagttcga cccgagcggg atactgcctt ccggccttct
     1321 tcccgcgatc gggagataac catcaggaat acggcatgag ccttttgatt gacccgaaag
     1381 ccctccgcaa atgggcttca aacgatcttg atgtccagaa aaaccaggat gggtcttacg
     1441 atttcctgtt tcgttatgaa ggctcttcgt gtcgaaacgg gggtagagca tttccggcgg
     1501 aaatccgcgt ctgtctctca cccgcagatg atggagactg gcgcatcaaa gacttcgcga
     1561 taatcgtgcc gccagcgggg cgcgaaggat gggaggctac gtgcattttc ggggaaatcg
     1621 gaaaggacag cgtcaggaga atggggactt cagttccgtt tcgaggacaa agtctcgatg
     1681 aagtcctcgc atccgatgtg gcactcaacc acgctggatg cttctgctca cccgcccacg
     1741 tcaaccacaa agccctgctc gctctctgga cgatcaaatg gtggctggac gatacggcca
     1801 aaatgtaagg gccggacttg aaaagcgccc gagatcgcgt tggccgggcg tggtggggaa
     1861 ccgtattttg gggatattgg gaatttcaga catttcgcct aagagggcaa cgccgtgtat
     1921 acgatcgtga taagtcagaa aaacccggaa atggacgcgc atgcgagagg taccctcgca
     1981 gacgtacgcg atcacggact caccgggatc acccgtgtgg aagtggatcg aattttcagg
     2041 gtcgagggag agctttcgcg ggaggctgcc gaacttctcg cgaaccggct gtttgttgac
     2101 ccggtggtgg agacgtgggc ggtgtacggc gcgggagaga cccctgacag gcgaggggcc
     2161 aaccacgtgc ttgaggtcgc ctacaacccc ggtgtcatgg acccagtgga aggatctgtt
     2221 ctgaaggctt ccggtgacct cggtgttgcg ctgcgtgccg tgcgcacagc cgcaagatac
     2281 cgtttctacg gagatgcgac cgcctctgaa atacttgagg ccgcgtatgc ccttctcgcg
     2341 aacaagaccg tgaaccatgt agtgatgggg aatgatgacc tccccaagcc gttccccgct
     2401 gaggaattcc ggggtccctc ccacgtagag ctggtcggct gcgatgatga aggcttgatg
     2461 gggatcagtc gcgggcgcgg gttatccctg agcgtatccg aaatgcggcg catcaaggaa
     2521 tacttcgaac acctcggcag gaatcctact gacatagaac tggaaaccct ggctcagacg
     2581 tggtccgagc actgtgtgca caagacgttc aaagggagaa tagaattcga cggtgaagta
     2641 atcgacaacc tgctcaaaca gacaatcgtg aaggcgaccc aggagcttgc gaagccctgg
     2701 tgcgtgtctg tgtttgatga caacgctgga gtgatcgagt ttaacgagaa gaatcatatc
     2761 tgtttcaaag tggaaaccca taaccacccc agcgcgctgg aaccctatgg cggcgccggt
     2821 acgggtatcg gaggggtaat cagggatgtt ctggggaccg ggctcggggc caaacctgtg
     2881 gcgaacaccg atgtgttttg ttttgcccgg cccgattacc cggcggacga gattccggaa
     2941 ggcatactcc atccacggcg gatcatgcgc ggcgttgttg cgggtgtacg ggattacggc
     3001 aacagaatgg gggtccccac cgtcaacggg gctgtcattt ttgatgatcg ctatctcggc
     3061 aatccgctcg tctactgcgg taccgtcggt ctgctgcccg tcggatgcga gcggaaaaca
     3121 gcgcttccgg gaatgaagat agttgttgtc ggcggacgaa ccgggcggga cggtattcac
     3181 ggtgcgacat tcagcagcgc agaattaggg agtgacgagg agaacgtctg gtctaatgcc
     3241 gttcagatcg gcaaccccat cacggaaaag aaagtcacgg atgtactc
//
LOCUS       DAOSOO010000297         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29333_length_3276_cov_5.044707, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000297 DAOSOO010000000
VERSION     DAOSOO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29333_length_3276_cov_5.044707"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2306
                     /gene="ppdK"
                     /locus_tag="PLT86_14090"
     CDS             <1..2306
                     /gene="ppdK"
                     /locus_tag="PLT86_14090"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT37787.1"
                     /translation="FAWDSYRRFVQMYGDVVLGLKPQSKDEEDPFEVIIKAKKAEKGV
                     KNDTELTTEDLKDLVVKFKAAVKQRKGMDFPEDPKEQLWGAINAVFGSWMGNRAIIYR
                     RINKIPEDWGTAVNVQAMVFGNMGEDSATGVAFTRDPATGEKVFYGEFLINAQGEDVV
                     SGVRTPSPIAELAKTQKAAYEQLEDIRAKLEKHYKDMQDIEFTIERNRLFMLQTRNGK
                     RTAQAAVRIAVDMVKEKLITKEAAVARIDPDQLDQLLHPMFDPAAKKKATVLAKGLNA
                     SPGAAVGKVYFNAEDAEAAAGRGEKVILVRLETSPEDIGGMAASQGILTVHGGKTSHA
                     AVVGRGMGKPCVVGCGALSVSYTERRFNVTVGGQTITVKEGDVISIDGSDGSVMLGAV
                     PTKDPEITGDFSMLMKWVDEIRTMGVRTNADTPHDAKVALDFGAEGIGLCRTEHMFFG
                     GDRIDAVREMILADDLAGREKALAKILPMQQEDFVGIFRVMNGLPVTIRTLDPPLHEF
                     LPNTDDQIEELAYKINRNPMRIREKLHQLHELNPMLGHRGCRLGMVYPEITAMQARAI
                     MQAACQVKAEGIDVHPEIMIPLVGTMGEMTNQKDLVVKVAKQVMKETGVTVNYMVGTM
                     IEVPRAAIIADKIAKEAEFFSFGTNDLTQMTFGYSRDDAGKFLPFYIENKVWIDDPFQ
                     SLDQEGVGKLVEMGVKLGRKGRKNLKIGICGEHGGDPASVQFCFRVGMNYVSCSPFRV
                     PIARLAAAQAALAAKDASTAGASKSTM"
BASE COUNT          692 a         1008 c          978 g          598 t
ORIGIN      
        1 gattcgcgtg ggatagctac cgccgcttcg tgcagatgta cggcgacgtc gtgctcggcc
       61 tcaagccgca gtccaaagac gaagaggatc ccttcgaagt catcatcaaa gccaagaagg
      121 cggaaaaagg tgtcaagaac gacaccgagc tgacgaccga ggatctcaag gacctcgtgg
      181 tgaagttcaa agccgccgtg aagcagcgca aaggcatgga cttcccggaa gaccccaagg
      241 agcagctctg gggagccatc aacgccgtgt tcgggtcgtg gatggggaac cgcgccatca
      301 tctaccgccg cattaacaaa atccctgaag actggggcac cgccgttaac gtgcaggcca
      361 tggtgttcgg caacatgggt gaagattccg ccaccggcgt tgccttcacg cgtgatcccg
      421 ccaccggtga gaaggttttc tacggagagt tcctgatcaa cgcgcagggc gaagacgtcg
      481 tatccggcgt gcgcacgccc tccccgatcg cggaactggc gaaaacccag aaagccgcat
      541 acgagcagct tgaggacatc cgcgcgaaac tcgagaagca ctacaaggac atgcaggata
      601 tcgagttcac cattgagcgg aaccgcctgt tcatgctcca gacgcgcaat ggcaaacgca
      661 ccgcgcaggc ggccgtccgg atcgcggttg acatggtgaa agagaagctc atcaccaaag
      721 aagcggctgt cgcccgcatc gatcccgatc agctcgacca gttgcttcac ccgatgttcg
      781 acccggcagc gaagaagaag gccaccgttc tggcgaaggg cctgaacgcc tcccccggcg
      841 cggcagtcgg caaggtgtac ttcaacgcgg aagacgcgga agccgccgcg ggacgcggtg
      901 agaaggtcat cctcgtccgt ctcgagacca gccccgagga catcggcggt atggcggctt
      961 cgcagggaat cctgacggtg cacggtggga aaacctccca cgccgccgtt gtcggccgtg
     1021 gcatgggcaa gccctgcgtc gtcggttgcg gcgcgctgag cgtgagctac accgaacgtc
     1081 ggttcaacgt gacggtcggc ggacagacga tcaccgtcaa agaaggcgac gtgatctcga
     1141 ttgacggttc cgacggttcc gtcatgctgg gagccgttcc gacgaaggac ccggaaatca
     1201 cgggcgactt cagcatgttg atgaagtggg ttgatgaaat ccgcaccatg ggcgtccgca
     1261 ccaatgctga caccccgcac gacgccaagg tagcgctcga tttcggcgcc gagggtatcg
     1321 gcctctgccg caccgagcac atgttcttcg gcggcgaccg cattgacgca gtgcgcgaaa
     1381 tgattctcgc ggacgacctc gcgggccgcg aaaaggcgct ggcgaagatt ctgccgatgc
     1441 agcaggaaga cttcgtcggc atcttccgcg tgatgaacgg cctgcccgtc accatccgca
     1501 cgctggatcc gccgcttcac gagttcctgc cgaacaccga cgatcagatc gaggaactcg
     1561 cctacaaaat caaccgcaac ccgatgcgca tccgggaaaa gctccaccag ttgcacgagc
     1621 tgaaccccat gctcggccac cgcggttgcc gtctcgggat ggtgtatccg gaaatcaccg
     1681 ccatgcaggc ccgcgccatc atgcaggccg cctgccaggt gaaagcggaa ggcatcgacg
     1741 tccatccgga aatcatgatc ccgcttgtcg gcaccatggg tgaaatgacg aaccagaaag
     1801 acctcgtcgt gaaggtcgcc aagcaggtga tgaaggaaac cggcgtgacg gtgaactaca
     1861 tggtcggcac gatgatcgaa gttccgcggg ccgccatcat cgccgacaag atcgcgaagg
     1921 aagccgagtt cttcagcttc ggaacgaacg acctcaccca gatgaccttc ggctacagcc
     1981 gtgacgacgc cggcaagttc ctgccgttct acatcgagaa caaggtctgg attgacgatc
     2041 cgttccagtc gctcgatcag gaaggcgtgg gcaagctcgt cgagatgggt gtgaagcttg
     2101 gccgtaaggg ccgcaagaac ctcaaaatcg gcatctgcgg cgaacacggc ggcgacccgg
     2161 catcggtgca gttctgcttc cgcgtcggca tgaactacgt ttcgtgctcg ccgttccgtg
     2221 tgccgatcgc ccgtctggcg gcggcgcagg ctgcgttggc ggcgaaggat gcctccaccg
     2281 caggcgcgag caagtcaacc atgtaaatca cacagcctgt gcaggtaagc ggtcttacag
     2341 agaactcgcg ccctgcacac aataagcgaa gccgcaccgg agcgatcctc gggtgcggct
     2401 tcgttgtttt ttgcccgatc tatcgcggaa cggcgatcta tcagtccatt agtcccatgc
     2461 gacccatccc cccggcgcgg tcacatttcg tgttctgtgc ggccctgtgc acaccctccc
     2521 agaaaccctg gtgcaaattt gcaacttctt cgcgaaaaag cttgacacga agacgacgtt
     2581 ttgccttagc tggtaggtgg gaaggataga cagactacat aacgggcgcc caccccggcg
     2641 tttcgacagg ctcaacgacc ggtgccattc cctttgtagg ggccgggttg cccggcccgt
     2701 tgcctgcatt cctccggcca cgacggagcg tgcctttcca acattcgcaa cgacgttgca
     2761 ggggccggac gcctttcttc gcgcactccg cgttctgctt tgttccggtc acgcccctcg
     2821 cacggtcgtc gagtggcggc tggagcggag cggaagccgt tgtatcgaga cgacccgctc
     2881 gctcgcccgt cagtgccctt cgcgtgcgtc atttcccctt ccccaaaaaa ttcccgttgc
     2941 aaatttgcaa cttcttcgcg aaaaagcttg acacgaagac gacgttttgc cttggctgga
     3001 gtgtgggagg cagatttcat gcgcccgtcg acgaattggt gcgcaggaga acacgggcac
     3061 gacggagcgt gcccctccac aggtcctgtt tgggtcccat aggatgtata ggaaacgccg
     3121 cattcgtggg attgccggag cggtcctcgt gtgcgctggt tttccgtcct tcgcggcttc
     3181 gtgtgcgtca ttgttcttgc cccgcaaaca accgtacccc gattgaacta ttttcgcgaa
     3241 aacccttgac acgaagacga cgttttgcct cagctt
//
LOCUS       DAOSOO010000298         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29407_length_3269_cov_5.487243, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000298 DAOSOO010000000
VERSION     DAOSOO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29407_length_3269_cov_5.487243"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="PLT86_14095"
     CDS             <1..1828
                     /locus_tag="PLT86_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT37788.1"
                     /translation="SGSDFVEMFVGVGASRVRDLFEKAKKKAPCIVFIDEIDAVGRHR
                     GAGLGGGHDEREQTLNQLLVEMDGFSSQSTVILMAATNRPDVLDPALLRPGRFDRHIV
                     VNMPDVRGREGILRIHTRGIPLARNVDLHTIARGTPGLSGADLRNLANEAALLAARNN
                     RSEVGMREFEIAKEKVMWGSERRSLVMSEEERRVTAVHEAGHALVAICHPVADSVHKV
                     TIVPRGRSLGLTAFLPGDERHTVSRHWCDAKLTSILGGRAAEEIVLGDITNGAANDIE
                     LATELARKMVCEWGMSKLVGPVALLRKGEEVFVAKELGRSAEVSEQTLQIIDKEVNRI
                     VEAALAKARDILEQRRTALDALSAALLAREVLDRQQIDALIAPLLEPPSSSPVSESAE
                     SVPKVIDRTGTDAAPSAVRSARPEKGPEARVRRSKRVEGSEAEQPSPVNQPESVQSEI
                     RAETAPPVREPVSVPAPSPPVMYGRRPKMVKRFALPTSQVPENGIPVASVVSEAPILY
                     GRSLRSEVRTKAQSQPLEAFPQAHASEGNSQGSREPEEGSTISRTVSGEQISAADMAE
                     NDRQEAIGGAQENTPQSADTNGDIEPGAADTLAKVDEAVGST"
     gene            1876..2625
                     /gene="cdaA"
                     /locus_tag="PLT86_14100"
     CDS             1876..2625
                     /gene="cdaA"
                     /locus_tag="PLT86_14100"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT37789.1"
                     /translation="MDITVADVLDVALLTFIFYKSFVLLRGTRSAPMVIGLIILLAIA
                     VIAERAEMVGSNWLLTQVKTVWLIGLVIVFQTELRRLLTYLGQNRLFRPFLAEAPDQT
                     VNEVVAACKTLSEQGIGALIVITRDVGLAGILETGTRIRAEVSASLIVSLFVPKTPLH
                     DGAVVISGNQIEAAKCILPLTQNAVDPSLGTRHRAALGVSEESDAVVIVVSEETSKIS
                     VAVDGVLERALSPEELRARLFRELNPQGRKA"
     gene            2733..>3269
                     /locus_tag="PLT86_14105"
     CDS             2733..>3269
                     /locus_tag="PLT86_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37790.1"
                     /translation="MPERLFPWVLLLILFFSPPSRGEVKVLNSDADGLDVEITAPLEI
                     DGANPAKLRIPGWETTSDPSQPGVPYLVVPIVVPSEGAVSASVTAQTDTFARCGPVEK
                     GSGDRPIGLDDEVAGAAAWSDPGRQLVSVEITGVARDVRMARLVVCPVSVVEGGVRWT
                     KSLRVAVRFASESTVPLAR"
BASE COUNT          654 a          909 c         1060 g          646 t
ORIGIN      
        1 cagtggctcc gatttcgtcg agatgttcgt gggagttggt gcctcgcgcg tgcgtgacct
       61 ttttgaaaag gcgaaaaaga aagcgccatg catcgtattt atcgacgaaa ttgacgcggt
      121 gggaaggcat cggggggctg gcctcggtgg cggacacgac gagcgcgagc agacgctgaa
      181 ccagcttctt gtggaaatgg atgggttcag cagtcaatcc accgtgatac tgatggcggc
      241 cacgaacagg cctgacgtac tcgaccccgc cctgttacgc ccggggcgat tcgatcgtca
      301 tatcgtggtc aatatgcccg acgtgagggg ccgtgagggg atacttcgca ttcacacacg
      361 aggtatcccg ctggcgcgca atgtggatct tcacaccatc gcaaggggca cgccgggtct
      421 ttcgggggct gacctccgga atctcgcgaa tgaggccgca cttcttgccg cgcggaacaa
      481 ccgcagcgaa gtcggcatgc gcgaattcga gatcgcaaaa gaaaaggtca tgtggggatc
      541 tgaacggcgc agcctcgtca tgtccgagga ggaacggcgt gtcactgcgg tgcatgaagc
      601 aggtcacgcc cttgtggcaa tctgccatcc cgtcgctgat tccgtgcaca aagtgactat
      661 tgtcccgcgc gggaggtccc tcgggcttac cgcgtttctg cccggcgacg agcgacacac
      721 ggtttcccgg cactggtgtg atgcgaaact caccagtatt ctcggggggc gcgccgccga
      781 ggagatcgtt ctcggggaca tcaccaacgg cgcggcaaac gatatcgagc ttgcgaccga
      841 gctggcacgg aagatggtct gtgagtgggg aatgagcaaa cttgtcgggc ccgtcgccct
      901 tctccggaaa ggcgaagagg tgttcgtcgc gaaagaactc gggcgttccg ccgaggtgag
      961 cgaacagacg ctgcaaatta ttgacaaaga ggtcaaccga atcgttgagg ccgcgctggc
     1021 gaaggcgcga gacattctgg agcagcgacg aacggccctt gacgcgctgt ccgcggcatt
     1081 gcttgcgaga gaggtacttg acaggcaaca gatcgacgcc ctgattgcac ctcttttgga
     1141 gccgccaagc agttctccgg tgagcgaaag cgcggaatcg gtcccgaagg tgattgaccg
     1201 aacaggcaca gatgcagcgc cctccgcggt cagatccgcg cgacccgaga agggtccgga
     1261 agcacgtgtc agacggagca aacgggttga gggttcagaa gcagaacagc cttcaccggt
     1321 aaatcagccc gaatcggttc agtccgaaat tcgggccgaa acagctccgc ccgtccggga
     1381 accggtgtca gtgccagcgc cgagcccgcc cgtgatgtac ggcaggcggc ccaaaatggt
     1441 caagcgcttc gcccttccga cgtcgcaggt tcccgagaac ggaatacccg ttgcgagtgt
     1501 tgtttctgaa gctcccattc tgtacgggag atccttgcgc tccgaagtcc ggacgaaggc
     1561 gcagtcgcag ccgctcgagg cgttccctca ggcccacgct tccgagggga attcccaggg
     1621 tagccgtgag cccgaagagg gaagtaccat ttcccgaacg gtttccggtg agcagatcag
     1681 cgcggctgac atggccgaga acgaccggca ggaagcgatc ggcggcgcgc aggaaaacac
     1741 tccgcagagc gcggacacga atggtgacat cgaacctggt gcggcagaca cgctggcgaa
     1801 ggttgacgag gcagtcggtt ccacctgaca aagcggacga ggcagaaaac gttcacactc
     1861 tttcatgtcg gtttcatgga catcaccgtg gctgatgtcc tcgacgttgc tctcttaaca
     1921 tttatctttt acaagagctt cgtccttttg cgcggtacgc gttctgcccc catggtgatc
     1981 gggctcatca ttctgcttgc gatcgccgtg atcgcggaac gtgcggagat ggtcgggtcg
     2041 aactggctcc tgacgcaagt gaaaaccgtc tggctcatcg ggctggtgat tgtgtttcag
     2101 acggagctgc gccggctgct tacgtacctt ggacagaaca ggctcttccg tccattcctc
     2161 gccgaggcgc cggaccagac cgtgaatgaa gtggtcgcgg catgcaagac cctctccgag
     2221 cagggaatag gggctctcat cgtcattacg cgcgacgtgg gactggccgg aattttggag
     2281 acgggtacgc gaatccgggc cgaagtgagc gcctcgctca tcgtgtcgct gtttgttccg
     2341 aagacgccgt tgcacgacgg ggcagtcgta atttctggga accagattga agctgccaag
     2401 tgcatacttc cactgacgca gaatgcagtg gatcccagcc tcggtactcg ccacagggcg
     2461 gcgctgggag tctccgagga atcagacgcg gtggtcattg ttgtgtccga agaaacgagc
     2521 aagatatctg tcgccgttga cggcgtgctg gaacgcgcgc tgtcgccgga agaactgcgt
     2581 gcgcgtcttt tccgtgagct gaatccgcag ggcaggaaag catgagcagg aaccatcgca
     2641 tcgtgtcgac attgttttta tcccgttctt ttcaatctga tgccgccttg tcggactgga
     2701 ctgccggagt ttctgcggga aggagccgcg tcttgcccga aaggttgttc ccctgggtat
     2761 tgctcctcat tctgttcttt tcgccgcctt cccgaggcga ggtcaaagta ctcaattcgg
     2821 atgcggatgg gctggatgtg gagataaccg ctcctcttga aattgatggt gccaatcctg
     2881 cgaagttgcg aattcccgga tgggaaacca cgtcagaccc gtcccaaccg ggcgtgccgt
     2941 atcttgttgt tcccatcgtg gtgccgtcgg aaggggcagt gagcgcatcc gtcacagcgc
     3001 agaccgacac gtttgcgcgg tgcggcccgg tggaaaaggg gagtggcgat cgtcccatcg
     3061 ggcttgacga cgaggtggca ggcgccgcag cgtggtcgga cccgggcagg cagctcgttt
     3121 cagtagagat tacgggcgtt gctcgcgatg tacggatggc gagactcgtc gtctgcccgg
     3181 tgagtgtagt ggaaggcggt gttcgctgga cgaagtcgct tcgcgtcgcg gtgcgctttg
     3241 cctcggagag cacggtgccg ctagcgaga
//
LOCUS       DAOSOO010000299         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29470_length_3263_cov_4.820761, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000299 DAOSOO010000000
VERSION     DAOSOO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29470_length_3263_cov_4.820761"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLT86_14110"
     CDS             <1..359
                     /locus_tag="PLT86_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37791.1"
                     /translation="AVMVGVQIVLFALVPSVSSVALFEAAFFVIMTCFGGGFATCPAF
                     VTELFGSRNTGAIYGAILTAWSAAGVAGPTMAAMLRDLTGSYSGGMYVIAGALAIALV
                     CVAALGASIRGRPAAV"
     gene            complement(411..2297)
                     /locus_tag="PLT86_14115"
     CDS             complement(411..2297)
                     /locus_tag="PLT86_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOT37792.1"
                     /translation="MEQRSLEHMILPPDVRKLLTDAPSVLCANRIEDLEALACGGREN
                     REWQVKYTLPDGKEVHEATVVRVKNGIAVNYTDPYMRRRDPDCMWIGDDRPSDKPRYA
                     ERFGRPFAELRTETLDWLSRQDLAVFAFEPGHLSIGMTGLAICPANAGFFAFGLGLLQ
                     GVVNLENRDEPFQPTFIIFVAPTFRHTHFGGRQAVVHNRVDVHEMFSYNLYPGPSAKK
                     GVYGALLSQGAREKWVTVHASCVLVRTPYDNMVTFMHEGASGSGKSEMLQQPHRLPDG
                     RLLLGRNLVTGEERYLEIPRTCDLHPVCDDMGLCHPDFQENDGRLWLTDAEDAWFVRV
                     DHITKYGTDHDLEQMTIAPARPLLFLSIDAVPNGTALIWDHVEDAPGVPCPNPRVILP
                     RDIVPGVVEGRVPVDIRSFGVRTPPCTAAAPSYGIIGLFHILPPALAWLWRLASPRGY
                     HNPSVVQAEQMGSEGVGSYWPFATGLRVEQANLLLEQFVRNRRMRHVLVPNQHIGVWR
                     TGFMPQWVARDYLARRGHAKFQSDQCRPARCPLLGYALQSIRIEGTTIPGWLFQVDQQ
                     PEVGTEAYDEGARILTEFFRRQVQKFLKNNLDELGRKIIDCFLANGTVEDYEKLLPVS
                     DGVY"
     gene            complement(2567..>3263)
                     /locus_tag="PLT86_14120"
     CDS             complement(2567..>3263)
                     /locus_tag="PLT86_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37793.1"
                     /translation="APPDMSNLPEAFPRRGIMHTGASSPFVCVAGMMPYQMFLEMCAT
                     ELGLLKELTEQCYERIRDVLEVIFSTRRIEFVWMGGCEWVTPPMAAHQVYEELVQPFE
                     AKLIAQMHAAGALSHVHCHGNVRSTLELAIARGADLFEPVEPPPDGDITFAEAKALAA
                     GRITLGGNIEARILENETPEDVEEASRAAFEGGKYRMIFQTTAGPLCDLTPRMIANYH
                     RMIDVWEELSPME"
BASE COUNT          590 a         1038 c          978 g          657 t
ORIGIN      
        1 tcgcggtgat ggtgggtgtt cagatcgtac tctttgccct tgttccgagc gtctcctccg
       61 tcgcgctctt cgaggctgcg ttttttgtca tcatgacctg cttcggcggc gggttcgcca
      121 cgtgtccggc gtttgtgacg gagctgttcg gttcccggaa caccggggcc atttacggcg
      181 cgattctgac cgcgtggagc gcagccggcg tggccggacc gaccatggcc gccatgttga
      241 gggacctgac ggggagttat tcgggcggca tgtacgtgat cgcaggtgcc cttgccatag
      301 cgctggtgtg cgtcgccgcg ctgggtgctt ccattcgtgg ccgccccgcc gcagtctgaa
      361 ggcacttcgg cgcacttgtc aacacgacgt tcacgtcacg cgggcgccga tcagtatacg
      421 ccgtcggaaa ccgggaggag cttctcgtaa tcttccaccg tgccgttggc gaggaaacag
      481 tctatgatct ttcgaccgag ttcatccaga ttgttcttca ggaacttctg aacctgcctt
      541 cggaagaact ccgtaagaat cctcgcccct tcgtcgtacg cctccgtgcc gacctccggt
      601 tgctgatcaa cctgaaacag ccatccgggg atcgtggtgc cctcgatacg aatactctgg
      661 agggcatatc cgagtagagg gcatctggca ggacgacact ggtccgactg gaacttcgcg
      721 tgaccccggc gggcgaggta atcgcgcgcg acccactgcg gcatgaatcc tgtccgccac
      781 acgccgatgt gctggttcgg taccagtacg tgccgcatcc ttctgttcct gacaaactgc
      841 tcgagcagca ggttcgcctg ttccacccgc agaccggttg cgaacggcca gtagcttccc
      901 accccttcgc ttcccatctg ctctgcctga acgacgctcg ggttgtgata tccccttggt
      961 gaagcaagcc gccagagcca cgccagagcc ggcgggagga tgtggaacag cccgatgatt
     1021 ccgtacgacg gcgctgctgc ggtgcagggc ggtgtccgta cgccgaaact tcggatgtcc
     1081 accggcacgc gcccctcgac aacgccgggg acgatgtctc tcggcagaat gacccgtgga
     1141 ttcggacacg gcacaccggg tgcgtcctca acatggtccc agatcagcgc agttccattc
     1201 ggaacggcgt caatgctgag gaagagaagc gggcgcgccg gcgcgatcgt catctgttcg
     1261 aggtcgtggt ccgtgccata cttcgtgatg tgatccacgc gcacaaacca cgcgtcttcc
     1321 gcgtcagtca accagagccg cccgtcgttc tcctgaaaat cggggtggca gagacccatg
     1381 tcgtcgcaga cagggtggag gtcgcacgtg cgggggattt cgaggtaccg ctcttctccc
     1441 gtaacgagat tcctgcccag cagcagccgc ccgtccggga ggcggtgcgg ttgttgcagc
     1501 atttcgcttt tgccgctccc gcttgcgcct tcatgcatga acgtgaccat gttgtcatac
     1561 ggcgtgcgca caagcacgca cgaggcgtgg accgtaaccc acttctccct cgctccctga
     1621 ctcagcagtg cgccatacac gccttttttg gcgctggggc ccgggtagag gttataggaa
     1681 aacatctcgt gcacatccac acggttgtga acgaccgcct gtctgccacc gaaatgggtg
     1741 tgccggaagg tcggcgcgac aaaaatgatg aaggtaggct ggaaaggctc gtcgcggttt
     1801 tcgaggttca ccacgccctg caacagcccc agcccgaacg cgaagaatcc cgcgtttgcg
     1861 gggcatatcg ccagcccggt cattccgatg ctcaggtgac cgggttcgaa tgcgaagacc
     1921 gcgagatcct gccgggaaag ccagtcgagc gtctccgtgc gcaattcggc aaacgggcgg
     1981 ccgaaccgct ccgcgtagcg cggtttgtca gaaggccgat cgtccccgat ccacatgcag
     2041 tccgggtcgc ggcggcgcat gtacgggtcg gtgtaattca ccgcgattcc gttcttcaca
     2101 cgcaccaccg ttgcctcatg cacctccttg ccatctggca gggtgtattt cacctgccat
     2161 tcgcggtttt cgcgtccgcc acaggcaagg gcttccagat cctcaatccg gttcgcacac
     2221 aggacggacg gcgcatctgt aagcagtttc cgcacgtccg gcggcagaat catgtgttcc
     2281 agactccgct gctccatttc aatccccaaa aaagcgcccc ggcagcacca ccgccggacg
     2341 acagacaaac gcgattcgcg tgctttcgca gggcaggatg ccctgccact ccgtgcgtca
     2401 ggagcggttc ctaatatcac acaaaaccgt caatttgaaa agtgccggag ggagaatccg
     2461 ctgctcaccg cggggaacgc atgaaatcgg ggtcaatcgg cgtgccggaa tgggttggga
     2521 agtgcttccg cgcgtacgct gcgtacgctt ctcgcccgac gcgaattcac tccatcgggg
     2581 ataactcctc ccacacatca atcatccggt ggtaattcgc gatcatgcgc ggggtgaggt
     2641 cgcaaagggg cccggcagta gtctggaata tcatccggta cttcccaccc tcgaatgccg
     2701 cgcgggaggc ttcttccacg tcttccggag tctcgttttc gaggattctc gcctcgatat
     2761 tgccgccaag ggtgatcctc ccggctgcga gcgctttcgc ctcggcgaag gtaatgtcgc
     2821 cgtcgggagg cggttccacc ggttcgaaca ggtccgcgcc ccgggcaatt gcaagctcca
     2881 gcgtggagcg cacgttcccg tggcaatgca cgtggctgag ggctccggcg gcatgcatct
     2941 gggcgatgag cttcgcctcg aatggctgca caagctcttc atacacctgg tgcgcggcca
     3001 tgggcggggt tacccactcg cacccgccca tccagacgaa ctcgatccgg cgcgtggaaa
     3061 agatcacctc cagcacgtcg cggattcgtt cgtagcattg ctcggtgagt tctttcagca
     3121 accccagttc cgtcgcgcac atctcgagga acatctggta cggcatcatg ccggccacgc
     3181 agacaaacgg cgacgaggct cccgtgtgca tgatcccccg cctcgggaac gcttccggca
     3241 ggttggacat gtcaggcgga gca
//
LOCUS       DAOSOO010000300         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_29946_length_3222_cov_5.838017, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000300 DAOSOO010000000
VERSION     DAOSOO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29946_length_3222_cov_5.838017"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLT86_14125"
     CDS             <1..1120
                     /locus_tag="PLT86_14125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT37794.1"
                     /translation="AARNGIAAREAGVEVRRASVAAEVYRLYYGVLVARELLEILDDV
                     SEKIAQARTRVREMLEEDSDRVTTTDLMRIDVYTYELERKHIEANKSVELALGALRRA
                     LGIPYDAHFDVVTDRLRPIPETLPSLDSLRAYAFQNRPELAQVNAAVEARRQQLIAAR
                     AERYPTPFVGVEVGLRAAPGRELLTENPFVGDAYNGRSVKAALGLQYTLDNRSREAGI
                     QRAEVEYREMLRKREWAEAGIALEVQKAYLEADEARRNSELGRRSLRAGSSLMIQTLE
                     RYDMGVASTRDLLEAYGAYAKSQGDYYQTLYSHYLALGEVYRTIGKPLSEITNGTNGV
                     GHSELEGEGDGDDAQFEEPVEPENSGENGEPYEPQPEE"
     gene            1089..1769
                     /locus_tag="PLT86_14130"
     CDS             1089..1769
                     /locus_tag="PLT86_14130"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT37795.1"
                     /translation="MNLTNHNQKNDATNDMRCIMWKTMALLAAVILGMAYTTQAAEQT
                     ALQALKQRQRAIEQAQAALARAPRDTARQAALRDAITEAFDFDRLARESVGRHWNAMT
                     PEQQRDYLTVFRSLVQRSTVRKLQRYRAAGTTYSAPTGNAQNTVITTIVTSTSGEKVA
                     IQYKMHKVNGRWWVWDTTIGLDTEISDYDVSTAENYRSAFNRIIRDEGIPGLIAKLRE
                     KERKGSDL"
     gene            complement(1853..2773)
                     /gene="ftsY"
                     /locus_tag="PLT86_14135"
     CDS             complement(1853..2773)
                     /gene="ftsY"
                     /locus_tag="PLT86_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT37796.1"
                     /translation="MISSIWSRLRHGLSRTRSTLTLRLRSILGGALTAETLETLEEVL
                     ITADVGVMTAAKILKRLETDAASSGGDPAWAMRSVRTTVEEILRAAHRPFALPEEKPA
                     VVLVVGVNGVGKTTTIGKLAARYAEQKRRVLIGAADTFRAAADEQLLVWAQRARAEIV
                     RQQHGADAAGVAYDAVAAAQHRSTDLVLIDTAGRLHTKSNLMEELRKIKRVLGKLSPT
                     FPHHVLLVLDATTGQNALVQARQFHEAIGVTGIALTKLDSSAHGGIVIAISDELRLPV
                     QFVGVGETLDDLQDFDPHAFTEALFDIGQD"
     gene            complement(2784..>3222)
                     /locus_tag="PLT86_14140"
     CDS             complement(2784..>3222)
                     /locus_tag="PLT86_14140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT37797.1"
                     /translation="SVIMLDVDHFKKFNDTYGHQLGDKVLKMVATVLLNVARRATDMV
                     ARYGGEEFVVIAPDTTMENARIVAERIRETLQESVIESEAGPLRVTASFGVASYPRCG
                     AETVEELIEQADEALYAAKRKGRNRVEEAASKNESSTLPTQPQ"
BASE COUNT          689 a          919 c          969 g          645 t
ORIGIN      
        1 agcagcccgg aacgggatcg cggcacgcga ggccggtgta gaagtgcgcc gcgcatcggt
       61 ggccgcggag gtgtaccgcc tctattacgg tgttctcgtt gctcgcgagc tgcttgaaat
      121 cctcgacgat gtgagcgaga aaatcgcgca ggccaggacc agagtcaggg aaatgctgga
      181 ggaggattcc gaccgggtta cgacaaccga tctcatgcgg atcgatgtgt acacgtatga
      241 actggagcgc aaacacattg aagcgaacaa aagcgtggaa ctggcgctcg gggcactgcg
      301 ccgcgcgctc ggcatccctt atgatgccca ttttgatgtt gttaccgatc gcttgcgacc
      361 gattccggaa acacttcctt cgctggactc gctacgcgca tatgccttcc aaaaccggcc
      421 tgaactcgct caggtcaacg cggctgtgga agccaggcgg caacaattga tcgcggcgcg
      481 cgccgaacgt tacccgacac cgttcgtcgg cgttgaggtt ggtctgcgcg ctgcccctgg
      541 gcgcgagctc ctgaccgaga acccgtttgt cggcgatgcg tacaacggcc ggagcgtcaa
      601 agcggcactc ggtttgcagt atacgttgga caaccgcagt agagaggcgg gaattcagcg
      661 tgccgaggtc gagtaccgcg aaatgctgcg caaacgagaa tgggcggagg cagggatagc
      721 cctcgaagtt cagaaggcct acctcgaagc tgatgaagcc cgcaggaaca gtgaattggg
      781 ccgcagatcg ctcagagccg gaagctcgct catgatacag acgctggagc ggtatgacat
      841 gggcgtcgct tcaacgcggg atctgctcga agcctacggg gcgtacgcga agtctcaggg
      901 cgattactac cagactcttt acagtcatta cctcgctctt ggtgaagtgt atcgaacgat
      961 tggaaaacct ctctcggaga ttaccaacgg aacgaatggg gttggccatt cggaactgga
     1021 gggcgaggga gacggagacg atgctcagtt tgaggagccc gtcgaacccg aaaattccgg
     1081 ggagaacggt gaaccttacg aaccacaacc agaagaatga tgccacaaac gatatgaggt
     1141 gtatcatgtg gaaaacgatg gccctgttgg ccgcagtaat actcggaatg gcgtacacca
     1201 ctcaggcggc cgaacaaacc gccttgcagg ctttgaagca acgacagcgt gctattgagc
     1261 aggcccaggc ggcgcttgcg agggctccac gagatacggc ccggcaggca gcacttcgcg
     1321 acgcaatcac agaagcgttc gattttgaca ggctggcacg cgaatctgtt ggccggcact
     1381 ggaatgcgat gaccccagag caacagagag attacctgac tgtgttccga tcactcgtgc
     1441 agcgctccac cgttcgcaag ttgcagcggt acagggccgc cggtacgacg tattcagcgc
     1501 cgaccgggaa tgcgcagaat accgtaatta cgacgatagt cacctccact tcgggcgaga
     1561 aagtggcgat tcagtacaag atgcacaagg tcaatggccg gtggtgggtg tgggatacga
     1621 ctatcggatt ggatacagaa atctcggact atgatgtcag caccgcggag aactatcgtt
     1681 ctgccttcaa caggatcatc cgggatgaag gcattcccgg gttgatcgcg aaactgcgcg
     1741 aaaaggagcg caaaggcagc gacctttagg tctccggaag ggcttgtttc cggaaaaacg
     1801 ggggaggcgg gaaatgcctc ccccgtttca tttggggccg gaatgtggaa cctcaatcct
     1861 gcccgatgtc gaacaaagcc tcggtgaacg cgtgcgggtc gaaatcctgg agatcgtcaa
     1921 gggtttcccc caccccgaca aattgaacag gcaaccgcaa ttcgtcggaa atcgcgatca
     1981 ctatcccccc atgggcgctg ctgtcgagct tggtcaacgc aattccggtt actccgatgg
     2041 cttcgtggaa ttgccgggcc tgaacaagcg cgttctgacc cgtcgtggcg tccagcacaa
     2101 gaagcacgtg atgcggaaac gtgggagaca gtttgccgag cactcttttg attttccgca
     2161 gctcctccat caggttggat ttcgtgtgga gcctcccggc agtgtcaatc agcaccaggt
     2221 ccgttgagcg gtgttgcgcg gccgcaactg cgtcatacgc cacaccggcg gcgtcagcgc
     2281 cgtgctgctg ccgtacgatc tccgccctgg cgcgttgagc ccagaccagc agttgctcat
     2341 cagccgccgc ccggaacgtg tctgctgctc cgatgagtac gcgccttttc tgctctgcgt
     2401 aacgggcggc aagtttcccg atcgtcgtgg ttttccccac tccattcacg cccaccacga
     2461 gcacgaccgc gggtttttcc tcaggcagtg cgaatggcct gtgtgcagcg cgcagtatct
     2521 cctctaccgt ggtgcggacg gatctcattg cccacgcggg gtcgccccca ctgctcgcgg
     2581 catccgtctc cagccgcttc aggatttttg cggcagtcat cacgccgaca tctgccgtga
     2641 taaggacctc ttcgagggtt tccagagttt cggcagtgag cgctccacca aggatcgagc
     2701 ggaggcgcag ggtgagagtg cttctggtac gggaaagccc atgtctcagg cgagaccaga
     2761 tggaggaaat catgcagata ccgtcactgg ggttgtgtcg gaagggtgga tgactcgttt
     2821 ttggaggctg cttcctcgac cctgttgcgc cctttccgct tggccgcata gagggcctcg
     2881 tcggcctgct cgatcaattc ctcgactgtt tccgctccac accttggata cgaagcgact
     2941 ccaaaactcg ccgtgactcg aaggggtcca gcttccgact cgatgacgga ttcctgcaga
     3001 gtctcccgga tacgttccgc aacgatgcgg gcattttcca tggtcgtgtc aggtgcaatg
     3061 acgacgaact cttctccccc gtaccgtgcc accatatccg ttgcgcggcg agcgacgttc
     3121 aaaaggaccg tcgccaccat tttgaggacc ttgtcgccca gctgatgtcc gtacgtgtcg
     3181 ttgaatttct tgaagtgatc cacatcgagc atgataaccg ac
//
LOCUS       DAOSOO010000301         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_30078_length_3209_cov_4.145212, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000301 DAOSOO010000000
VERSION     DAOSOO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30078_length_3209_cov_4.145212"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLT86_14145"
     CDS             complement(<1..590)
                     /locus_tag="PLT86_14145"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOT37798.1"
                     /translation="MYLSSLLINVGDNPDRPRPGRAWLRNRYRVHQRLCMAFPSDLRK
                     EGDPEFLAPYKPEDFPEQRHLADQKKNEVDGATLNQVHAPRNDATGFMFRVDPRSNGR
                     AVIIVQSAREPDWGYAFHNANYLLAAPPEVKPFEPRFSKDQCLRFRLAANPTRRLSRH
                     SPDAREESVGKRVPIPTDQLTDWLERRAESSGFIIKR"
     gene            complement(593..1714)
                     /gene="cas5e"
                     /locus_tag="PLT86_14150"
     CDS             complement(593..1714)
                     /gene="cas5e"
                     /locus_tag="PLT86_14150"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOT37799.1"
                     /translation="MTEANTLFLRLEGPLQAWGDTSKFVIRRTMDAPTKSGVLGLLCC
                     AMGLSREKARERLPELNGLAMGVRVDRPGTRWWDYQTVGAGIGMTTAGGGLKTGAQGT
                     LITRREYLADASFLVALQGDAKLIHDIASAIAAPKWPIFLGRKSCPPSVPVLAMPHEG
                     ESWTNPAGHDDLNAALGAVPWHPRHKTNATPQNGTVKTLVEWRPNSEGDAAPDDAEVW
                     YDVPVCFDPPVHEPRFVLRGEISPTVGEALQGPAPAMPRPRANYTNSEYRKRREERIQ
                     ADAGLCVFCKSPGPRMTVQHVTYRRAGGDETQEDLRSLCGLCHDAVTMIEYGRGMGLD
                     RINPEEPRWRDLIFQKRDDILRFRSLETRRRRLATEEVE"
     gene            complement(1711..2877)
                     /gene="cas7e"
                     /locus_tag="PLT86_14155"
     CDS             complement(1711..2877)
                     /gene="cas7e"
                     /locus_tag="PLT86_14155"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOT37800.1"
                     /translation="MLIEIHMIQNHSPANLNRDDLGAPKTCIFGGVTRARISSQCLKR
                     SIRRSPEFAAALEKDGGVRTRRLIELIANAAVGEGNQPNEQHLTAIRKVFTDGGIGLK
                     NENTFDILFFLPQSAINEMGAAVRSGNGDLGKKFAEIIGKLVKTPDIALCGRMTEFEA
                     KGSFKDLKGQFTVEAALSAAHAISTHEVVNEVDYFTAADDVRGQDAGAAHVNEAIFNS
                     ACFYKHFVIDFDQLKKNLANDADLALKTVRCFLEAAVKANPSGKQHAFAAFNPPDGVL
                     VEVKTKSATPVSYANAFADPVPEKCERGLIGESITRLGQYAHDLVAGYGIEADRFWFS
                     PNLRHRLTWIDTSKKQHEQEQSVVGSKEFGEFDQFVNKVMDKMMTTDTPAEATK"
     gene            complement(2877..>3209)
                     /locus_tag="PLT86_14160"
     CDS             complement(2877..>3209)
                     /locus_tag="PLT86_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37801.1"
                     /translation="TGPTLPEVLARCEPLDEDGRKRFRTRFDALLCSSLSSLEPHVRW
                     ALGEVALAVAGHVLHARDVKGLDWVQLLDDLSIWDRGEEHRRGRDVRDIWAETYLKSV
                     RQTYKEEE"
BASE COUNT          606 a          943 c          974 g          686 t
ORIGIN      
        1 cgcttgatga tgaatcctga agattcggcc cgacgctcaa gccaatcggt cagctggtcg
       61 gtcggaatgg gaacgcgctt cccgacggat tcctcccttg catccggtga atgcctgctc
      121 aggcgcctgg tcggatttgc cgcgaggcgg aatcgaaggc actggtcttt cgagaagcgt
      181 ggctcaaatg gtttcacctc agggggcgct gccagaagat aattcgcatt gtgaaaggca
      241 tacccccagt cgggttctct ggcagactgc acgatgatca ccgctcggcc gttggaacga
      301 ggatcgacgc gaaacatgaa accggttgcg tcattgcgcg gggcgtggac ttggtttagg
      361 gtggcgccgt cgacctcgtt cttcttttgg tccgccaggt gccgctgctc agggaaatcc
      421 tccggcttgt atggggcaag gaattcagga tcgccctctt ttcgcaaatc ggacgggaag
      481 gccatacaaa gacgttggtg aacacggtag cggttcctca accatgcccg tcctggccgg
      541 ggccggtcgg ggttgtcgcc gacgttgatc aagagactgg acaaatacat ggctattcca
      601 cctcctcggt ggccagacgc cggcgtcgag tctccagcga gcggaagcgg agaatatcat
      661 cccgtttttg aaaaataagg tcgcgccagc ggggctcttc aggattgatg cggtccaatc
      721 ccatgccacg gccgtattcg atcatggtga cggcatcatg gcacagaccg caaagcgaac
      781 gcagatcctc ttgggtttcg tcacccccgg cccttcggta ggtgacgtgc tggacagtca
      841 tgcgcggccc cggcgacttg cagaaaacac acaatccggc gtcggcttga atccgctctt
      901 cgcggcgctt gcgatactct gaatttgtat aatttgcgcg cggacggggc attgcggggg
      961 caggcccctg aagcgcctcg ccgaccgtcg gtgaaatctc accgcgcaag acgaatcgcg
     1021 gttcgtgaac cggcggatcg aagcagaccg gaacgtcgta ccatacttcc gcgtcgtcgg
     1081 gagcggcgtc accttctgaa ttgggcctcc attccacaag tgttttcacc gttccattct
     1141 gcggcgtcgc gttggttttg tgccgggggt gccatggtac ggcaccgagc gcggcgttca
     1201 ggtcgtcatg accggcggga ttcgtccacg actcaccttc gtggggcatt gccagcaccg
     1261 ggacggacgg cgggcaggac ttgcggccaa ggaaaatcgg ccacttcggc gcagcaatcg
     1321 ccgaagcgat atcatggatc aacttcgcat cgccctggag cgccacgaga aaactcgcgt
     1381 cggccaggta ttcccggcgc gtgatgagcg tgccttgggc gccggtcttc agaccacccc
     1441 cggctgtcgt catgccgatc cccgctccaa ccgtttggta atcccaccac cgcgtgccgg
     1501 gccggtcgac gcgcacaccc atcgccagcc cgttcagttc aggcaagcgc tcccgcgcct
     1561 tctcacgcga caggcccatg gcgcagcaga gcaggcccag cacgccggac ttggtcggcg
     1621 catccatggt acgccggatg acgaactttg acgtgtcacc ccaagcctgg agggggcctt
     1681 caaggcggag gaagagtgtg ttggcttcgg tcatttcgtc gcctccgcag gcgtatcggt
     1741 tgtcatcatc ttgtccatga ccttattgac gaactgatcg aactcaccga actctttgga
     1801 accgaccacg gattgttcct gttcgtgttg ctttttgctc gtgtcaatcc aggtcaggcg
     1861 atggcgcaga ttcggcgaga accagaagcg gtcggcctcg atgccgtaac ctgcaacgag
     1921 atcatgcgcg tactggccga gtctggtgat gctttcgccg atcaatccgc gttcgcactt
     1981 ttccggcacc ggatcggcga aggcgttggc gtagctgacg ggagtggccg acttggtctt
     2041 gacctcaacc agaactccgt cgggcggatt gaatgccgca aacgcgtgct gtttgcccga
     2101 gggattcgcc ttgaccgccg cttcaaggaa gcagcggact gtcttgaggg cgaggtcggc
     2161 atcgttcgcc aggttcttct tcaactgatc gaagtcaatg acgaagtgct tgtagaagca
     2221 ggccgagttg aatatggcct cgttgacgtg agccgcgccg gcgtcctggc cgcgcacgtc
     2281 gtcggcggcg gtgaagtagt ccacttcgtt cacgacctcg tgagtcgaaa tcgcatgagc
     2341 ggcggagagt gcggcctcaa cggtgaactg tcctttgaga tccttaaacg atcccttggc
     2401 ctcgaactcg gtcatacggc cacagagtgc gatgtcgggc gtcttgacta acttgccgat
     2461 aatctcagcg aatttctttc ccaggtctcc gtttccgctg cgcacggcag cacccatttc
     2521 gttgatggcg gattggggaa gaaagaagag tatgtcgaaa gtgttctcat tcttcaagcc
     2581 aatccccccg tccgtgaaga ccttccgaat ggctgtgaga tgttgctcat tcggttgatt
     2641 gccttcaccc accgcagcgt ttgcgatcaa ctcgataagc cgtcgtgtgc gcacgccacc
     2701 atccttttct aaagcggctg caaactccgg gctgcgccgg atgctgcgtt tgagacactg
     2761 gctggagatg cgcgcccggg tgacgccgcc gaagatgcag gttttgggcg cgccaaggtc
     2821 gtcccggttc aggttcgccg ggctgtgatt ttggatcatg tgaatttcga tgagcattat
     2881 tcttcctcct tgtaagtttg tctgacggac ttgagatacg tttcggccca gatgtcgcga
     2941 acatcgcgtc cgcggcggtg ctcctcaccc cggtcccaga tggagagatc gtcgaggagt
     3001 tgcacccagt ctaatccctt cacatcacgc gcatgcaaca catggccggc gacggctagg
     3061 gcgacttctc ccagcgccca gcgtacatgt ggttccagag acgacaggga cgaacaaagc
     3121 aaagcgtcga atcgagttcg gaagcgcttg cggccatcct cgtcgagcgg ttcgcagcgg
     3181 gccaggactt cgggtaaagt gggcccagt
//
LOCUS       DAOSOO010000302         3203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_30133_length_3203_cov_5.103240, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000302 DAOSOO010000000
VERSION     DAOSOO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30133_length_3203_cov_5.103240"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1334
                     /locus_tag="PLT86_14165"
     CDS             213..1334
                     /locus_tag="PLT86_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT37802.1"
                     /translation="MPDPRFDQLAQVLIGYSTALKEGENVLIEAAEAPDELVISVMRA
                     ARNVGARVFVNLFHQRVQREFVRLCSDEQMTLHGEIEKARMEKMQAYISIRGTSNISE
                     MSDVPREAMRIYERRFLQPVHFDVRVKQTKWVALRYPTPSMAQLAQMSTEAFEDFYFR
                     VCTLDYARMAAAMVPLRELMEKTKLVRIVGPDTDLRFSIEGIPVIACTGQNNIPDGEI
                     FTAPVRDSVNGKIAFNARSPYHGINFESIRLEFDRGKVVRASANNTQKLNEILDADEG
                     SRYVGEFAIGVNPFIRRAMGDILFDEKIAGSIHLTPGNAYEEADNGNRSDIHWDMVLL
                     QDADSGGGKLYFDDVLVRQDGEFVLDFLRPLNPDELSKS"
     gene            1446..2573
                     /locus_tag="PLT86_14170"
     CDS             1446..2573
                     /locus_tag="PLT86_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT37803.1"
                     /translation="MEYRAYGNTGLKLSLLGFGCMRLPMKDEHVVEDEAIRMLRYAID
                     HGVNYIDTAFFYCNHESEVVVGKALKDGYREKVTLSTKNPVHEPDGKKWRQTLDEQLR
                     KLDVDKIDVYHFHGIGWDEWNEKFIVPGGPADEMRRAQKEGIVTHCAFSFHDKPENLI
                     KLVDTGFFEGVLLQYNLLDRANEAGIAHAHEKGLGVVVMGPVGGGRLAAPSDGIKKLV
                     PGPVQSSAEAALRFVMGNPGVTVALSGMSTMEQVKENLATAETAGVLSDADRAQIAKN
                     LDEVKALSDLYCTGCNYCMPCPHGIDIPRNFELMNYYRVWGLKDYAKERYARLKERRV
                     DDSIVEAWAAACTQCGECEPKCPQNIPIPEQMKEVAATLGT"
     gene            2655..>3203
                     /locus_tag="PLT86_14175"
     CDS             2655..>3203
                     /locus_tag="PLT86_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT37804.1"
                     /translation="MNLSSQVSSFFQNSLRAMKSRNYRLFFFGQGVSLIGTWIQRVAM
                     SWLVYRITSSAMLLGTVEFVGMLPTFLLAPFTGVLADRWNRHTIMLGTQIGFTLSALA
                     LGLLVVTEIVQVWHILVLSAISGIVMAFDVPARQSLVVDMVEDKRDIGNAIALNSTMF
                     NGARLIGPSIAGLIIAAFGEWIC"
BASE COUNT          666 a          942 c          975 g          620 t
ORIGIN      
        1 attgccttgc tgccgcctgt ctgtgcggcg tatcaatgca tcgtgccggc acttcgaccg
       61 gcttcctgcg ccttccgcag gctctgcgac cgcctcccga cgaccagcat ccatcatcag
      121 cacgcaaaat gaggggcgcc ctctccgcgt tgcgtatctt caggtacgct gcccggacac
      181 atcacgcgac cctccacaac gagaggctcc ccatgcccga tccccgtttc gatcagctcg
      241 cacaggttct gatcggctat tcgaccgccc tgaaagaggg cgaaaatgtc ctcatcgagg
      301 ccgcggaagc gcccgatgag ctcgtgatct ccgtgatgcg ggccgcgcgc aacgtcggtg
      361 cgcgggtgtt cgtgaacctg ttccatcaac gcgtgcagcg cgaattcgtg cgcctctgct
      421 cagatgagca aatgaccctc cacggcgaaa tcgaaaaggc gcgcatggag aaaatgcagg
      481 cgtacatttc catccgcgga accagtaaca taagcgagat gtccgacgtc ccgcgcgaag
      541 caatgcgcat ttatgagcgc aggttccttc aaccggtgca tttcgatgtg cgggtgaaac
      601 agacgaaatg ggtcgccctg cgctacccga cgccatccat ggctcaactt gcgcagatga
      661 gcactgaagc attcgaggat ttctacttcc gggtatgcac gctcgattat gccaggatgg
      721 cggccgcgat ggtcccgttg cgggaactga tggagaagac gaaacttgtc cgaatcgtcg
      781 gccccgacac tgacctccgg ttctcgatcg aaggcattcc cgtcatcgcc tgcacggggc
      841 agaacaacat ccccgacgga gagatcttca cggcgccggt aagggattcg gtcaacggga
      901 agatcgcctt caacgcgcgc agcccgtacc acggcatcaa tttcgaaagc atccggctcg
      961 aattcgacag agggaaggtt gtcagggcca gcgccaacaa cacgcagaaa ctgaacgaga
     1021 ttctggacgc agacgagggt tcgcggtacg tcggcgagtt cgccatcggc gtcaacccgt
     1081 tcatccggcg tgcgatgggg gatatcctgt ttgacgaaaa aatcgcgggt tcgattcacc
     1141 ttacccccgg caacgcgtac gaggaggcgg acaacggcaa ccgctcggac atccactggg
     1201 acatggtgct gcttcaggac gccgacagcg gagggggaaa gctgtatttc gacgacgttc
     1261 ttgtccggca ggacggggaa ttcgtgctcg atttcctcag gccgctgaac cccgatgaac
     1321 tgagcaagag ctgacgggag gccgtccgtt ctttggcccg gggtgtgtgg ccgacgtaca
     1381 ttttgttcat gtgacacttt cgccggattt tgagccgttg tcaacggcgc gcaggaggat
     1441 gattgatgga gtatcgagcg tacggaaaca caggactcaa gctttcgttg cttggtttcg
     1501 ggtgcatgcg gctgcccatg aaagacgagc atgtcgttga ggacgaagcc atccggatgc
     1561 tccggtatgc aatagaccac ggggtcaact acatcgacac ggcgtttttt tactgcaacc
     1621 acgaaagcga ggttgtggta ggcaaggcgc tgaaggacgg ttaccgcgag aaggtgacgc
     1681 tttcgacaaa gaaccccgtg catgaaccgg acggcaagaa gtggcgccag acgctggatg
     1741 agcagctccg caagctcgat gtggacaaga tcgacgtcta ccacttccac ggcatcggct
     1801 gggacgagtg gaacgagaag ttcatcgttc ccggtgggcc tgcggacgaa atgcggcgtg
     1861 cgcagaagga agggatcgtt acccactgcg cgttttcgtt tcacgacaag cccgagaacc
     1921 tcatcaagct tgtggatacg ggcttctttg agggcgtgtt gctccagtac aacctgctcg
     1981 accgcgcaaa tgaggccggc attgcccacg cccatgaaaa agggctcggc gtggtggtga
     2041 tgggacccgt tggcgggggg aggctcgcag ccccttcgga tggcatcaag aagctcgttc
     2101 cggggcccgt gcagagctcc gcggaagccg cgctccggtt tgtcatgggc aaccccggcg
     2161 ttacggtggc gctttccggc atgagcacca tggagcaggt gaaagagaac cttgcaaccg
     2221 cggaaactgc cggagtgctt tcggatgccg accgggccca gatcgcgaag aacctggatg
     2281 aagtgaaggc gctgtccgac ctgtactgca ccggctgcaa ctactgcatg ccgtgtcccc
     2341 acggaatcga catcccgcgc aacttcgagc tcatgaacta ctaccgcgtg tgggggctca
     2401 aagattacgc gaaggagcgt tacgcgcgcc tgaaagagcg cagagtagac gacagcattg
     2461 tcgaagcctg ggctgccgcc tgtacgcaat gcggcgagtg cgaacccaag tgcccgcaga
     2521 acattcccat cccggaacaa atgaaggaag ttgccgcaac actcggcacc tgaccccgct
     2581 cttcaggcac cgcgaggaag ggggcgcaac ctcaagacgc cccctttcct ttccctgcga
     2641 gggtaacccg gaacgtgaat ctttcctctc aggtctcctc ctttttccag aacagcctcc
     2701 gagcgatgaa aagccgaaat taccggcttt tcttcttcgg gcagggcgta tcgctcatcg
     2761 gcacgtggat ccagcgcgtc gccatgagct ggctggtgta ccgaatcacc agttccgcca
     2821 tgttgctcgg aacggtggag ttcgtcggca tgctgccgac gttcctgctg gcccccttca
     2881 ccggtgtttt ggccgaccgg tggaaccggc acaccattat gctgggaacg cagatcggct
     2941 tcacgctgag tgcgctcgcg ctcgggcttc tggtggtgac ggagatcgtt caggtgtggc
     3001 atatcctcgt gttgagcgcg atctccggaa tcgtgatggc gttcgacgta ccggcccgcc
     3061 agtcgcttgt ggtagacatg gtggaggaca aaagggacat cgggaatgcg atcgcgctga
     3121 actccacgat gttcaacggc gcacgcctca tcgggccctc catcgctggt ctcatcatcg
     3181 ccgcgttcgg tgagtggatc tgc
//
LOCUS       DAOSOO010000303         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_30577_length_3168_cov_5.281401, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000303 DAOSOO010000000
VERSION     DAOSOO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30577_length_3168_cov_5.281401"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1021..>3168)
                     /locus_tag="PLT86_14180"
     CDS             complement(1021..>3168)
                     /locus_tag="PLT86_14180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37805.1"
                     /translation="TETAAAAASTLTLSIANAGDVTGESGSPATGVNSTGTATSTTRR
                     FPRLRALLAGATPAAAALADNAENVPVLLVNLNSTAEAITINSLTLTSTGTATLGTEV
                     TAIKLWNDSDADGVLDTGEPQIGSTWTTGDLLFNLSGVQVPSGGNFAVLVTVNTGTGV
                     AGHTVRLAVGIGGAVGTGAVTASALTNPAAATGNLMTFNGLLVSQGDTPVAANSSVGI
                     SSTIPVFQFKLSAIGEAVSVTQVVVSGSGTADLPTDFSAVRLYHDANGDAVVNGGDTQ
                     LSGDRTFSSGSVTFGSISGFNLSSGSSANMLVTVTTSGTGVSGNVFSVNIASNAAITQ
                     SGTRVSLSTTPLASNTHTFAQGVVTVSGTDVAPTSINPGDDNVKMMRLTFTTSGPAVQ
                     LSSVRVDNSYLGGSGNFSYLDIQFLKIFRDDGDQVFEPGTGDVLINGAGTAWTSTPSP
                     GGSATVEFGTPDMLYASAAKDYWVAYDIHPDAVSTRQIGVRLADNTYVSTGGVGTVAT
                     TNFAIQLNTEHSLPVELASFTAEAVGQVVKLAWVTESETDNLYWIVERKEASAADATF
                     VQVHRVDGMGSTPYRTEYEFVDRSVVLGTRYAYRLTDVSRFGVRKTHDPVEVTAGAAL
                     TLSLGTAFPNPANPTTTWAYSLPADQNVRMAVFNTLGQQVRVLVNGTMPAGVHQATWD
                     TRDDMGRELASGVYICVLQTPTSRLLSRVTIVR"
BASE COUNT          572 a         1003 c         1030 g          563 t
ORIGIN      
        1 tgccccgtga cgtactggct gaggcaaaga gtcctgttcg tgtcaaggct tttcgcgaaa
       61 atgtttcaat cggggtacgg ttttttttct ggggggtgga ggggcacgct tcgtcgtgcc
      121 tgccgaccag cggctgcgtg ccgccggacg gctgccttga agcgccaacg gcgcgcgata
      181 gcggagcccg gttcgcagga ccgggcgagg gggtgcctgc atggacccga cggagcgggt
      241 ccctccaggg ccggtggtca ggtcttcgtg gacggggtgg acagagtgga ccgggctggc
      301 gcaagggccg ggcggtgaga tgatgaacgg tcatctcgat acaacgaacc ggcggagaac
      361 gtcgcgttgc gtcccccctc cccccggcgg ttcgccactc gatgaccggt gagggtcgag
      421 tcgttggaca tcaaccgaca gtcaaaacgc ggcgcgaacc cgacggagcg ggtccctcca
      481 gacgggaggg tgcgtgtggg gcacacgcgc gccggtctcc gagtggcgga cgcgaagcgg
      541 acgctgtatc gaggagcccg tcgcgcagca ccaggtgagg gcgtgcctgc gcggacccga
      601 cagttggaac gcggcgtgga cctgacggag cgggtccctc cagggcaccc ggccctttga
      661 gccgggctgg actatagcgg cccttcaggc cttggtggac gcgcaaggat tcttgaagcg
      721 ccgacggcgc gcgatagcag agcccggttc gcaggacggg gcgagggcgt gcctgcgcgg
      781 acccggcagt tagaacgcgg cgcggacccg acagagcggg tccctccaga cgggagggtg
      841 cgtgtggggc acacgcgcgg cggtctccga gtggcggatg cgaagcggac gcagtatcga
      901 ggagcccgtc gcgcaggtcg cccggccaac ccggcccggg ggggcgaaag aaaaaggggc
      961 gacctgcgtg cgaaattccg cacggaccga tcgcccctgc tcaagatgtt ctcgaaaggt
     1021 ttaccgcacg atggtcaccc ggctcagaag cctgctggtg ggggtctgca ggacgcagat
     1081 atacacgccg ctggcaagct cacggcccat gtcgtcgcgc gtatcccacg tggcctgatg
     1141 cacacccgcc ggcatggtgc cgttgacaag cacgcgcacc tgctggccaa gcgtattgaa
     1201 caccgccatc cgcacgttct gatccgcggg gaggctgtac gcccacgtgg tcgtcgggtt
     1261 cgccgggttc gggaatgccg tgccgaggga cagcgtgagc gccgcgccgg ccgtcacttc
     1321 caccgggtcg tgcgttttgc gcacaccaaa ccggctcacg tcggtcaggc ggtatgcgta
     1381 tctcgttccc agaaccacgc tgcggtccac aaactcatac tccgtgcgat acggggtgga
     1441 gcccatgccg tccacacggt gcacctgcac gaaggtcgca tcggcggcac tggcttcctt
     1501 ccgctcgacg atccagtaca ggttatccgt ttcgctttcc gttacccacg cgagtttcac
     1561 cacctgcccg acggcttccg cggtgaagga ggcaagctcc acggggaggg agtgctcagt
     1621 gttgagttgg atcgcgaaat tcgtggtcgc cacggtgcca acgcctccgg ttgagacgta
     1681 cgtgttgtct gcgagtcgta ccccaatctg ccgtgtcgac acggcatccg ggtgaatatc
     1741 atatgctacc cagtagtcct tggctgcgct ggcatacagc atgtccggcg tcccgaactc
     1801 gacggtagcg gatccgccgg gactcggggt ggacgtccac gcagtcccag ccccattgat
     1861 gagcacatcg cccgtccccg gttcgaacac ctggtcgccg tcatcccgga agatcttgag
     1921 aaactggatg tcgaggtagc tgaagttgcc actgcccccg agatagctgt tgtctacgcg
     1981 gacgctggaa agctgcactg ccgggccgga ggtggtgaaa gtgagccgca tcatcttcac
     2041 gttgtcgtca ccggggttga tcgaagtcgg cgcaacgtcc gtccccgata ccgtgaccac
     2101 gccctgcgcg aacgtatgcg tgttggaagc gagaggcgtc gtggaaaggc tcacacgcgt
     2161 gccagattgc gttatcgccg cgttggaagc gatgttcact gaaaacacgt ttccgctcac
     2221 cccagtacca cttgtggtta ccgtgacaag catgtttgcc gacgaaccag aactgagatt
     2281 gaaaccagaa atcgaaccga atgtcacaga accgctggaa aacgttctat caccgctgag
     2341 ctgcgtatcg cccccattca ctaccgcatc accattcgcg tcgtgataga gtctgaccgc
     2401 gctgaaatcg gtcggcagat ccgccgtccc cgaacccgac accaccacct gcgtcaccga
     2461 caccgcttcg ccaatcgcgg agagtttgaa ctggaacacc ggtatcgtgg aactgattcc
     2521 caccgagctg ttcgccgcca ccggcgtgtc gccctgggat acaagcagcc cgttgaaggt
     2581 catcagattg ccggtcgctg cggcagggtt cgtcaacgct gaggcggtca ctgccccggt
     2641 acctactgcc ccgccgatgc ccaccgcaag gcgcacggtg tgcccggcaa cacctgtccc
     2701 ggtgttcacc gtgacgagca ccgcgaaatt gcctcccgat ggcacctgaa ccccgctgag
     2761 gttgaacaac agatcgcctg tagtccaggt tgaacctatc tgtggttcgc ccgtgtccag
     2821 aaccccgtcc gcatcggagt cgttccacag cttgatcgcc gttacttccg tgcccagagt
     2881 cgccgttccg gtcgaggtca gcgtcaacga gttgatcgtg attgcctcgg cagtcgagtt
     2941 cagattcacc agcagcacgg gcacgttttc cgcgttgtca gccaaggccg ctgccgcggg
     3001 agtagcgccc gccagaagcg cccgcaaccg cgggaatctc cgggtggtgg acgtcgcggt
     3061 gccggtcgaa ttcaccccag tcgccggcga gccgctttca ccagtcacat cgcccgcatt
     3121 cgcgatggaa agggtcaaag tggatgccgc agcggcggct gtttctgt
//
LOCUS       DAOSOO010000304         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_31910_length_3067_cov_4.506640, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000304 DAOSOO010000000
VERSION     DAOSOO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31910_length_3067_cov_4.506640"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..1124
                     /locus_tag="PLT86_14185"
     CDS             381..1124
                     /locus_tag="PLT86_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOT37806.1"
                     /translation="MLRYRRERKVAGATLVKVQPRASRYPLWMRIGSSDHLPFTQIFV
                     QREYQPLDQLDDVRLVLDCGANAGYASAYFLSRFPQAKVIAIEPDPGNFEILERNTRV
                     YGQRIDCLQAGVWPYRAGLKLEEVPFRSGREWSRQVREIREGETADFPAIDIGALLAK
                     SGYDRISILKMDIEGAETLVFSKNFESWIDRVDAIAVELHNDSQFGCATEAFLSAIEG
                     RGFTVTTCGELTIAKRLTATGTADSVCDA"
     gene            1395..2965
                     /locus_tag="PLT86_14190"
     CDS             join(1395..1869,1869..2965)
                     /locus_tag="PLT86_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612171.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOT37807.1"
                     /translation="MPYPQSFKEAMVQKMLGPTGASANALAEKVGVPQSSLSRWLREA
                     RTLDPMKKSKKQAQEPRSPREWTMEEKLKAVISAAELSGQEFGAFLRREGLHEAQLQQ
                     WRDALQAALGAANPKKRTRASAEAKRIKQLERELRRKDKALAEVTALLVLKKKLEAFL
                     GGKGRRHPSNERRMILEGIKEAVRDGARVARACEIVGLSERAYQRWNKDPDGGEDRRK
                     GPTSPPVNQLTEEERVAVVETLNSWEFRNQSPKQVVPILAERGTYIASESSMYRILKE
                     EGLNAHRAASRPPRHTKPREFVAQGPGEVLTWDITYLHSPVRGAFFYLYLVVDVWSRK
                     ILGGAVHDAESSAYASALISTVIEENGLSPETVVVHMDNGSPMKGATLRATLEALGVQ
                     ASYSRPHVSNDNPYSESLFRTLKYRPEYPSKPFQSLEAAQQWVAGFEVWYNTQHRHSA
                     IGYVTPEQRHSGVAHAVLARRRQVYEAARRRNPARWTGDIRRWEAPDVVYLNPEDPLN
                     TVTARSASRPAEAVA"
BASE COUNT          657 a          846 c          987 g          577 t
ORIGIN      
        1 cgagctgacg tcgagccagc cgaggggtgc tcgctcgatg aaatcgtctc ggaccctttc
       61 gcgctgaagc tgccttcatc gcgtccccct ccgcccgtcc gccaggcctc cggcccagcc
      121 gcctgctccc gcgccccctg attctgcagc gctgggctcg catactgcgc tttcggtgta
      181 ggttgattgt tgcgtgtttc gcagttataa tctgttcatg gatgaagaaa acggatgaga
      241 caaaacgcga acggcatctc atacgtgcga ttcggattca gttctgacca attttttcaa
      301 tgattacaga taaaattaaa tattcgttta atcgcgtcag aacctttctg gggtttgcgc
      361 gtgagcttgg gtgggttggc atgttgcgtt accggcgaga gaggaaggtg gctggcgcaa
      421 ccctcgtgaa agtgcagccc cgagcgagcc gttatccatt atggatgcgc atcggctcgt
      481 cggaccattt gccgttcacg cagatcttcg tacaacggga atatcagccg ctggatcagt
      541 tggacgacgt tcggctggtg ctcgactgcg gcgcgaacgc agggtatgcg tcggcatact
      601 ttctgtctcg atttcctcag gcgaaagtga ttgcaatcga accggatccc gggaatttcg
      661 agatactgga aaggaacacg cgggtgtatg gtcagcgaat cgactgcctc caggctggcg
      721 tgtggccata ccgcgcgggt ttaaagctag aggaagtacc gtttcgcagc gggcgagaat
      781 ggtcgcggca ggttcgagaa atccgcgagg gggagacagc ggattttccc gcaatcgaca
      841 ttggggcttt gcttgcaaaa tccggatatg atcggatttc tattctgaaa atggatatcg
      901 agggagcgga aacgctcgtg ttctcgaaga attttgaatc gtggattgac cgggtggacg
      961 ccatagcggt ggaacttcat aacgattcgc aatttggttg tgcgacggag gcatttctgt
     1021 cggcgatcga ggggcgtggg ttcacagtca caacctgcgg cgagctaaca attgcgaagc
     1081 ggttgacggc aactggcacg gcggatagtg tgtgcgatgc ttaggtcgga gaaaccggga
     1141 ctcgcctcac gtcaggcgtg agcagctaaa gaagcgcatc gaactcattg gcggtgacaa
     1201 gtgaaaagga aagtgtgtct ttccagtgag cgcgcgccgc cccccgtgtc aggatagttg
     1261 tcgggcgggg tgaaaccgct gggacgccat ggggcgttcg aggcggggac cgcccgcccc
     1321 tcccccgggg gaggggcgac actttagaat ttggtaaaca aggcaactga ggggcggaga
     1381 aagggccgga gacaatgccc tatccgcaat cgttcaagga agccatggtc cagaagatgt
     1441 taggacccac cggggcctct gccaacgcct tggcagaaaa ggtcggggtg ccgcagagtt
     1501 cgctctcgcg gtggctcaga gaagcgcgta ccctggaccc catgaaaaag agcaagaagc
     1561 aagctcaaga accacggagt cctcgagagt ggaccatgga ggagaaactc aaggcggtca
     1621 tctcggccgc cgagttgtcc ggccaggagt ttggcgcctt cctgcggcgc gaaggactgc
     1681 acgaggctca actgcagcag tggcgtgacg ccttacaggc agccttgggc gctgcgaacc
     1741 cgaagaaacg tacacgagct agcgcggagg ccaagcgcat caagcagctc gagagggagc
     1801 tcaggcggaa ggataaggcc cttgccgaag tgacggccct cctggtgctc aaaaaaaaac
     1861 tggaagcgtt ctgggaggca aaggaaggcg gcacccaagc aacgagcgac gaatgatcct
     1921 cgagggcatc aaggaggctg tccgcgacgg cgcacgtgtt gcgcgtgcgt gtgagatcgt
     1981 agggctcagc gagcgggcct accagcgctg gaacaaggac cccgatggag gcgaggatcg
     2041 ccgcaagggc cccacgtccc cccctgtaaa ccaactcacg gaggaagagc gggttgcggt
     2101 cgtggaaacg ctgaactcct gggaatttcg caaccagtct cccaagcagg tcgtgccaat
     2161 tcttgccgag cgaggtacct atatcgccag tgagtcgagc atgtaccgta ttctcaagga
     2221 ggaaggcctc aatgcccacc gggcggcgtc ccgacctccg cggcatacga aaccgcgcga
     2281 gtttgtcgct cagggtcccg gtgaggtact cacgtgggat atcacctacc tccacagccc
     2341 tgtccgcgga gcattcttct acttgtacct ggtcgtcgac gtctggagtc gcaaaatcct
     2401 gggtggggcg gtgcacgacg ccgagtcgag tgcgtacgcg agcgcactga tttctacggt
     2461 gatcgaagag aacggattgt ccccagagac ggtggtggtc cacatggata acggttcgcc
     2521 gatgaaggga gctacactgc gagcgacctt ggaggcgttg ggggtacagg cttcctacag
     2581 tcgcccgcac gtgagcaacg acaatccgta ctccgaatca ctgttccgga cgctgaagta
     2641 ccgcccggag tacccctcga agcccttcca gtccctggaa gccgcccagc agtgggtggc
     2701 aggcttcgag gtgtggtaca acacgcagca ccgccacagc gcgatcgggt acgtgacgcc
     2761 ggaacagcgt cattcaggag ttgctcacgc tgtccttgca aggcggcgcc aggtgtacga
     2821 ggccgcacgg cgcagaaatc ccgcgcgctg gactggagac attcggcgct gggaagcccc
     2881 cgacgtcgtc tacctcaacc cggaggatcc gctcaacacg gtgacggcac gttcggcgtc
     2941 acggccggcg gaggctgtgg catgaaatag gtttaaccga caactctctt gacgcgcgcc
     3001 gcttcccggt cgtcgggcca tgatccagcg tagcggctac gagaccgcgt ccgctggcgg
     3061 cgttgaa
//
LOCUS       DAOSOO010000305         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_31912_length_3067_cov_4.031541, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000305 DAOSOO010000000
VERSION     DAOSOO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31912_length_3067_cov_4.031541"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..863
                     /locus_tag="PLT86_14195"
     CDS             87..863
                     /locus_tag="PLT86_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37808.1"
                     /translation="MRRQLIAPTRILVALLLPALCVAAEQSEGEAREAPSPPMSVMVS
                     VERTGAAAILGTNSAGSSTEFAITAKWLVLVFEADYRDMGWSRRSLVAPAGLSGSTEW
                     GSLTRIAPGIQYYQDIGRRWAVWGKFSAIAGFEETPSLRSVTWNPQLVALRTLKDGTV
                     LYCGAGSLYHRASKTFFPIVGVARNTDARQGFSAVIAFPEAYARYRFSERVALRADYE
                     WDTRVFGLSANNPTAQDGYLRSATITPLSTLSTPRHGRRR"
     gene            1351..2859
                     /locus_tag="PLT86_14200"
     CDS             1351..2859
                     /locus_tag="PLT86_14200"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HOT37809.1"
                     /translation="MTPELLAAARTTARLINTRIQIAIPSILAEGETFAARISVTGSD
                     ALPVDASDLVLHFEGAVGTAGLPPVFRFGAGESTGRIDGLTATGPEVALVRARVEVPG
                     RTGQISSNPAWVFANPPYRLFFGDLHVHTAFSNCSGWRCLPPDWCYEYAREISLLDFV
                     APADHLRGIASDPQRWPGLQASAKKHNAPGEFVTFLAFESSHAQGFGGDNNAYYLDDD
                     APCFWLDREDMRGTSPQVHLKELWRFLDGTGKAYFTAPHHTGRAAKYRAWDEDYYDAR
                     REPLFEIYSSWGSSEKRHSRLPISGGNNDAPAYFVDALKAGTRFGVIASSDDHATLPG
                     AAHQFRVDAFRVPTLNGHAHKGLAAVRAPELTRSALFDAMRSRQTYATTHARSLVDFH
                     IGDAAVGEEVRADAGLKARRTIRVRYTPDAASATRIALVRNGEELETKALRGPEIAAA
                     ANEVEFVDAEPLERVALKGAKFHPAPFVVYYARIEDGNGAHQWTSPIWIDLE"
BASE COUNT          488 a          974 c         1045 g          560 t
ORIGIN      
        1 aggtgtgtat gggtcgggtt gcccgacccc cttatctttt ctggcaagag aacgaagcgc
       61 gagtgaagga aagggggtga gccacgatgc gacgccaatt gattgccccc acccggatac
      121 ttgtagccct gttacttccc gcgctctgcg ttgccgccga acagagcgag ggagaggcgc
      181 gcgaagctcc gtccccgccg atgtcggtga tggtgagcgt ggagcgaacc ggcgctgcgg
      241 ccatccttgg caccaattcc gccgggtcgt ccaccgagtt cgccattacg gcgaagtggc
      301 tcgttctcgt attcgaagct gattaccggg acatgggctg gagccggcgt tcgttggtgg
      361 cgcccgcggg tttgtccggc agcacggagt ggggctctct cacacgtatc gcgccgggca
      421 ttcagtatta ccaggatatc ggcaggcggt gggcggtctg gggcaaattc tcggccatcg
      481 cggggttcga ggaaaccccc tcgttgcgct ccgtgacgtg gaatccgcag ctcgtggctc
      541 tccggacact gaaggacggc accgttctct attgcggcgc gggttccctc taccaccgcg
      601 cgagcaaaac ctttttcccg atcgtcggcg tcgccaggaa caccgatgcg aggcaggggt
      661 tttccgccgt gatcgcattc cccgaggcgt acgcgcggta ccggttcagc gagcgtgttg
      721 cgctgcgggc ggactacgag tgggatacgc gtgtattcgg gctttctgcc aacaacccga
      781 ccgcacagga cggctacctc aggtccgcga ccattacccc gctctcaacg ttgagtacac
      841 cccggcacgg aaggcgacgg tgacggccgg ggttcgctgg ctgggtggcc gcgacgtgac
      901 actgtacgat cgtgacgcgc gcatggtgtc tgcgcacgat gtggaggcgg cctgggcctt
      961 ctccctgagt ctgctgttgg ggtcgtaggc acgggccgcc attgttgccg gcgcgggctc
     1021 ctcgatacaa cgctcactgc gccttccaaa ctccgcggac ttcgtcgccg ccgcgtcact
     1081 cggagacccg cgcgccgggt agcgggcgcg attatggacg gcccctggcg ccagcatcgt
     1141 cattcccgcg caggcgggaa tccaaccgtt ttctctgcaa cgcgttccct gctgtaccgc
     1201 gttgcttcag ggccggccag gaatagactc cgggccgggg tttggcgccg acgcgcggaa
     1261 acagccgcgt tcggattgcc gcgtcttgag cgcgcaccta tcttcgactc tgtgaaaatg
     1321 ctgcgccgcg cgtccttaca ggagttgcac atgaccccgg aattgctcgc cgccgcccgc
     1381 accaccgcgc gcctcatcaa cactcgcatc cagattgcca taccctcgat cctcgcggag
     1441 ggggaaacgt ttgcggcgcg gatcagtgtc accgggtcgg atgccctgcc ggtagacgcg
     1501 tccgatctgg ttctccattt cgagggagcc gtcggcaccg ccggattgcc accggtgttt
     1561 cgtttcggtg cgggggagag cacgggcaga attgacggcc tgaccgccac cggccctgag
     1621 gtcgcgcttg tccgtgcgcg ggtggaggtg ccgggccgca cggggcaaat ttcctccaac
     1681 cctgcgtggg tgttcgcaaa cccgccctac cggctgttct ttggcgatct gcacgtccat
     1741 acggcatttt cgaactgcag cggctggcgc tgcctgccac ccgactggtg ctacgaatac
     1801 gcccgcgaaa tctcgctgct ggacttcgtc gctccggcgg accacctccg gggcatcgcg
     1861 tctgatccgc aacggtggcc ggggcttcag gcgtccgcga agaagcacaa tgcgccgggc
     1921 gagttcgtca cgtttctggc gttcgagagc tcccacgcgc aggggtttgg cggcgacaac
     1981 aacgcctact acctcgacga cgatgccccc tgcttctggc tggacaggga ggacatgcgg
     2041 ggcacttcgc cgcaggtgca tctgaaggag ctgtggcgtt ttctggacgg aaccgggaaa
     2101 gcctacttca ccgcgccgca ccataccggc cgggcggcga aataccgcgc atgggacgag
     2161 gactactacg acgcacggcg tgagccgctc ttcgagatat actcgagctg ggggtcatcg
     2221 gaaaaacgcc attcgcggct gcccatcagc ggtggcaata acgacgcgcc ggcgtacttc
     2281 gtggacgcgc tgaaagcggg cacgcggttc ggtgtgatcg cctcaagcga cgaccacgca
     2341 accctgcccg gcgccgcgca ccagttccgc gtcgacgcat tccgggtgcc gaccctgaac
     2401 ggccatgcgc acaaggggct ggcggcggtc agggcgcccg agttgacccg gtccgcgctc
     2461 ttcgatgcca tgcgcagccg gcagacgtat gccaccaccc atgcgcgctc gctggtggat
     2521 ttccacattg gcgacgcggc cgtgggcgag gaagttcgtg ccgatgccgg cttgaaggcc
     2581 cggcggacaa tccgggtgcg ctacaccccg gatgccgcct ctgccacccg tattgcgctg
     2641 gtgcgcaacg gggaggagct ggagacgaag gcgctgcggg gacccgagat tgcggcggcg
     2701 gcgaacgagg tggagttcgt ggacgccgaa cccttggaac gcgttgccct gaagggcgcg
     2761 aagttccacc ctgcgccgtt tgtggtctac tacgcgcgca tcgaggacgg caacggggcg
     2821 caccagtgga caagcccgat ctggattgat ctggagtgag gggccgaggg cggtctctgg
     2881 ttctatcagt ggtggaacac gcccggccgg tggtgcgcgc cgtttccggg caacgcgtgc
     2941 atgcatctgg aaccgtttgt ggcggagcag ctggcggtgg gtgaatccgc atccatccgg
     3001 ggtcgcgtcg gcatgcacca gggtgatcgg gcgtcgctgg aggggttgtt cgggggcttg
     3061 ggggcac
//
LOCUS       DAOSOO010000306         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_32099_length_3054_cov_3.870290, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000306 DAOSOO010000000
VERSION     DAOSOO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32099_length_3054_cov_3.870290"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1847
                     /locus_tag="PLT86_14205"
     CDS             69..1847
                     /locus_tag="PLT86_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOT37810.1"
                     /translation="MFLKCSMRRKDGKEHRSWSVVESRRVAGHRVVQRHVLYLGEIND
                     SQRAAWEKSLAVFDEQRQESLTCALFPEDRTPPPVLDVTAVQVRLAALRLERPRSWGA
                     CWLADGLWRELKLDEFFAARLSVSREGTDWEKVLRILAIYRLVSPGSEWRLHRHWFET
                     TALADLLGVDAGAVADDTLYRCLDLLVAHKEELFAHLRQRWSDLFGARYEVLLYDLTS
                     TYFECDAPGDENDPRRFGYSRDRRGDCVQVVVALVVTPEGLPLAYEMMPGNTADKTTL
                     RAMVELIGQRYGKAERIWVMDRGIPTEEVLAELRASVPRVQYLVGTPKGGLTKLEAAL
                     AQRPWQVVRPSLRVKLLPQEGELYVLAESRARAGKERGMRQRRLKSYWHRLGELAGQE
                     LMRDELLQKLGAARDRAGRVAAGLVRVNVSAAGVLSYQLDRVKLRVARAREGRYLLRT
                     NLSVNDPETVWRCYMQLVAVEEAFRTLKGDLGLRPIFHRKAGRIEAHLFVAFLAYCLA
                     TTLRQQLRAAAGGLMPRVVLEKLATVQLLDVILPTTDDRELLLVRRTEPTRDVALLLE
                     RMGRRLPAQPPPRLRAMGGITKVEPM"
     gene            complement(2520..>3054)
                     /locus_tag="PLT86_14210"
     CDS             complement(2520..>3054)
                     /locus_tag="PLT86_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOT37811.1"
                     /translation="EVRGMPQRNLAVEMLRKLIEGEIRARGRKNIVQARSFAELLGAA
                     IRKYQNRAIETAQVIEELIRLARDMREATRRGEELGLTEDEVAFYDALEVNDSAVSVL
                     GDETLRLIAQELVKSVRNSITIDWTVRENVRAQMRVIIKRILRKYGYPPDKQARATEL
                     VLEQAEVLCQDWSTGSG"
BASE COUNT          473 a          845 c         1123 g          613 t
ORIGIN      
        1 gtttcaagga cgtgtagtgg cgacctgccc ccttgcgtgc cttcttctga tgccggtatt
       61 ctgcggacat gtttctgaaa tgcagtatgc gccgcaagga cggcaaagag catcgctcgt
      121 ggtcggtggt ggagagtcgc cgcgtcgccg gccaccgcgt ggtgcagcgc cacgtgctct
      181 accttggcga gatcaatgat tcgcaacgcg ccgcgtggga gaagtcgctc gccgtcttcg
      241 atgagcaacg ccaggagtca ctgacgtgcg cgttgtttcc cgaagatcgc acgccgcccc
      301 cggtgctgga cgtgacggcg gtgcaagtgc ggttggcggc gctgcggctg gagcgaccgc
      361 ggagttgggg cgcgtgttgg ttggccgatg ggttgtggcg ggagttgaaa ctggatgaat
      421 ttttcgcggc gcggttgagc gtaagccggg agggaacgga ctgggagaag gtgctgcgga
      481 ttctggcgat ttaccggctg gtgtcgccgg gcagcgaatg gcggttgcac cggcactggt
      541 ttgagacgac ggcgttggcg gatttgctgg gcgtggacgc gggggcggtg gccgacgata
      601 cgttgtatcg gtgtctggat ttgttggtgg cgcacaagga ggaattgttc gcgcatctgc
      661 ggcagcggtg gagtgatttg ttcggagcgc gttacgaggt gttgctctat gatttgacga
      721 gcacgtactt cgagtgcgat gcgccgggcg atgagaacga tccgcgccgg tttgggtaca
      781 gtcgggatcg gcgcggggat tgtgtgcagg tggtggtggc gttggtggtg acgccggagg
      841 ggttgccgtt ggcgtacgag atgatgcccg gtaacacggc ggacaagacg acgttgcggg
      901 cgatggtgga gttgattggg cagcggtacg ggaaagcgga acgtatctgg gtgatggatc
      961 ggggcattcc gacggaggaa gtgttggccg agttgcgggc gagcgtcccg cgcgtgcagt
     1021 atttggtggg cacgccgaag ggggggctga cgaagctgga ggcggcgttg gcgcagcggc
     1081 cgtggcaggt ggtgcggccg tcgttgcggg tgaagttgct gccgcaggag ggcgaactct
     1141 atgtgttggc cgagagccgc gcccgcgccg gcaaggaacg cgggatgcgg cagcggcggc
     1201 tgaagagtta ctggcaccgg ctgggcgaat tggccgggca ggagttgatg cgcgatgagt
     1261 tgttgcagaa actcggcgcg gcccgcgacc gggccgggcg tgtcgcggcc gggctggtgc
     1321 gggtgaacgt gtcggcggcg ggcgtgttga gttatcaact ggaccgcgtg aaactgcggg
     1381 tggcgcgcgc gcgggagggc cggtatctgt tgcggacgaa tctgagcgtg aacgatccgg
     1441 aaacggtgtg gcgctgctac atgcagttgg tcgcggtgga ggaggcgttt cggacgttga
     1501 agggcgatct ggggctgcgc ccgatctttc accgcaaagc cgggcggatc gaggcgcatt
     1561 tgttcgtggc gtttctggcg tactgcctgg cgacgacgct gcggcagcaa ttgcgggcgg
     1621 cggccggcgg gttgatgccg cgggtggtgc tggagaagct ggcgacggtg cagttgttgg
     1681 acgtgatcct gccgaccacc gatgatcggg aactgctctt ggtgcggcgc accgaaccga
     1741 cgcgggatgt ggcgctgttg ctggagcgaa tggggcggcg tctgccggca caaccgcctc
     1801 cgcgattgcg agcaatgggc gggatcacga aagtagagcc gatgtagtgg cgacgtttga
     1861 gttgcttgag cgatgaatca agcacttacg tcgttgcacc ccgccagtag cggaagtcgg
     1921 gctaacggcg cttcggaaag gcctctgcgg gcggcccggg acgtgctcat gctgagagtc
     1981 gttcggctag ggttgcccct ggggagccaa attatttcgc ggaaggtttt cgctccatcc
     2041 ggttaacacg actgcccgaa cggcattctg gtcgggcgtg caatttttcc gttgattcca
     2101 cgacgcgacc cgcaaccagc cgacaaggct tcccgagcag cgccgccgcg aggctgcggc
     2161 cattctcgcc cggcgcccgc tggttcggcg ttggaaactg ccggccctcg gcgaggctgc
     2221 ccgccggcgt ccgcctggaa agcagccttt gccctcattc gcagccggga gtagaacatc
     2281 cgtgccaact gcccgagcgc gcgcgggtag cgaaaatcag caaggcccgt tcggcgtcat
     2341 ccgctggcat gcagacccgg caccgctggt gctgctttgg tttccggtgc gggctcatgg
     2401 cattcagcag tttgtgacgt ttgcggccag aggctcgccc gcgccggcgc ggcgcgcgca
     2461 gcggcagctt gctatttact ggctcgcgca gttgtccagg ccggatgggc cccatttcat
     2521 tacccgctac cggtgctcca gtcctggcac agcacctcgg cctgttcgag aaccagttcg
     2581 gtcgctcgcg cctgtttgtc cgggggataa ccgtacttgc gcagaatacg cttgatgatg
     2641 acgcgcatct gggcacggac gttctcgcgc accgtccagt ctatggtgat gctgttccga
     2701 accgacttca ccagctcctg ggctatcagc cggagcgtct cgtcgccgag caccgatacg
     2761 gcactatcat tgacctccag ggcgtcgtag aaggcgacct cgtcttccgt caaccccagt
     2821 tcctcgcccc gacgcgttgc ctcgcgcata tcacgagcca gacgaataag ctcttcgatg
     2881 acctgcgcgg tctcgatggc acggttctgg tacttgcgga tggcggctcc cagcagttcg
     2941 gcaaaggacc ttgcctgtac gatgttcttc cgcccgcgcg ctctgatttc cccctcgatc
     3001 agcttgcgca gcatctccac cgccaggttc ctttgcggca tgccgcgcac ttcc
//
LOCUS       DAOSOO010000307         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_33711_length_2940_cov_5.644367, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000307 DAOSOO010000000
VERSION     DAOSOO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33711_length_2940_cov_5.644367"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..384)
                     /gene="cas2"
                     /locus_tag="PLT86_14215"
     CDS             complement(91..384)
                     /gene="cas2"
                     /locus_tag="PLT86_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOT37812.1"
                     /translation="MADDGRYVVCYDVTDDKRRLKIVKLLEGYGDRVQYSVFEMAISP
                     DQLSELVEKLLGQLDAGADRATVYAICAACERRVIRLGRAADVVPGEEEVFIA"
     gene            complement(519..1574)
                     /gene="cas1"
                     /locus_tag="PLT86_14220"
     CDS             complement(519..1574)
                     /gene="cas1"
                     /locus_tag="PLT86_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOT37813.1"
                     /translation="MPSLYVTEPGSTVRLSGDSLVVTAEIDPDGKGPEPARREELLEV
                     EPHRVELIALVGRCNITADAMHLCLERGIAVAWFSRNGKYLGRVSPQMARSADIRLRQ
                     YAIAADAAQRLARAREVVAAKLANASEVIECLRSNLPGNRRMADTLQELGQYRAKARG
                     SATIEHLLGTEGIASRSYFGAFGEGFKGGIGFPGRTKHPPTDPANALLSFGYVLLCNL
                     IAGALEARGLDPAIGFYHELRPGRPSLALDLLEELRHPSVDRFVLRICNLGILKPEHF
                     ELDDSESLPGENGVRLTRDGLKVFFREWEKHLATPVRDKETGGAISVAEILRRQVDRL
                     AASLRGTEAYKSFLFGD"
     gene            1745..2677
                     /gene="cas6"
                     /locus_tag="PLT86_14225"
     CDS             1745..2677
                     /gene="cas6"
                     /locus_tag="PLT86_14225"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOT37814.1"
                     /translation="MVTLRVAQLEAVFTALEPLRLPPFIGSTLRGAFGHALKSAVCVM
                     RARECHSCQLSRGCLYTRIFETDPEGADLRHLGVTDNAPRPYVFAPGMGTPEILQPGD
                     TLSLTMRLFGWAVDYSSYVMYALSLLAERGLGAARGRLQLTRVIDGTTGLAAYDCEDG
                     DVYEPIKFVRLPVPIPAAGQGDVTVLWQTPLRVVNAHQLVQEPTAPVVMRTLLRRVSG
                     LLTYHENAALPYNAQEVLAATDRVAVKRNSTNWYDWKRYSNRQNRAVEMGGILGKTTY
                     EDTEGVLRPWLTLAETIHLGKSCTFGLGAVRIVA"
     gene            2688..>2940
                     /locus_tag="PLT86_14230"
     CDS             2688..>2940
                     /locus_tag="PLT86_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37815.1"
                     /translation="MTDKEAVVLGALLHDVGKFWQRTSARRVDTGMDAYVCPPGGTHV
                     HAVWTYRFFEELGAGTPGSTGQWLGLARDAAAFHHNPRGD"
BASE COUNT          505 a          902 c          970 g          563 t
ORIGIN      
        1 ggagtccagt ttgggcctcc ttcggagtcc agtttgggcc tccttcggag tccagccccg
       61 aacgtcgttt cgcgtgaaag aaggcggcgg tcaggcgatg aacacctctt cttcgccggg
      121 aacaacgtcg gcggctcggc cgagccggat tacgcggcgc tcgcaggcgg cacagatcgc
      181 gtacaccgtg gcccggtcgg cacctgcatc cagttgcccg agcagcttct ccacgagctc
      241 ggaaagctgg tcgggcgaaa tggccatctc gaagacgctg tactgaaccc ggtcgccata
      301 gccttcgagc agcttgacga tcttcaggcg ccgcttgtcg tccgtcacgt catagcagac
      361 gacatagcgt ccgtcatcag ccaccggcgt ttctccgatt ggataggagt atagtggaac
      421 gttctgcgtt tgtcaacggc gtggggaacg aacgcaccgg ggcttcaaca ggctcccgcg
      481 ggcgggggca cgctcagcca ccggcgccaa aacagttttc aatccccgaa aagaaatgac
      541 ttgtacgctt ccgtgcctcg caagcttgcg gccagccggt ccacctgtcg gcgcagaatt
      601 tccgccacgg atatcgcgcc tccggtttcc ttgtcgcgaa cgggcgtggc gaggtgtttc
      661 tcccactcgc ggaagaagac tttcaggccg tctcgtgtga ggcggactcc gttctccccc
      721 ggaaggcttt cgctgtcgtc gagttcgaaa tgctccggct tcagaatgcc gaggttgcag
      781 attcgcagga cgaagcgatc cacggatggg tgcctgagtt cttcgaggag gtcgagggcg
      841 aggctcgggc ggcccgggcg gagttcgtgg tagaaaccga tggccgggtc gaggccgcgg
      901 gcttcaagtg ccccggcgat caggttgcag agcaagacat agccgaacga gaggagcgcg
      961 ttggcggggt cggtgggagg gtgcttcgtt ctgccgggaa acccgatccc gcccttgaat
     1021 ccttctccga aggcgccgaa gtatgagcgg gaagcgatac cctcggttcc cagcaggtgc
     1081 tctatagtcg cgcttccccg cgccttagcc ctgtattggc ccaattcctg cagtgtgtct
     1141 gccatgcggc ggttgccggg cagattgctc cggaggcatt cgatgacttc ggaggcgttg
     1201 gcgagcttcg cggcgacgac ctcccgggcg cgggcgaggc gctgcgccgc atccgcggcg
     1261 atggcgtact gccggagacg gatatccgcc gagcgggcca tctgcgggct cacacggccg
     1321 aggtatttgc cgtttcgcga gaaccaggcg acggctattc cccgttcgag gcacagatgc
     1381 atcgcgtcgg cggtgatgtt acaccggccg acgagcgcga tgagttcgac gcggtgcggc
     1441 tcaacttcga ggagttcttc ccggcgggcg ggttcgggcc cctttccgtc ggggtcaatc
     1501 tccgccgtga cgaccagact atctcccgac aggcggaccg tggaaccggg ctccgtgacg
     1561 tacaacgatg gcacaggacc tccccggggc aacttcaggt cgcttcgatt cgcgcttttg
     1621 cgggcattcc gcctatcgcg cagtaaatat ccttccggcc cccttgcgtt tgttcactcc
     1681 cgtagttaaa atagaaccga accacagggg gagcgtactg ttaccacatg cctggggagg
     1741 cggcgtggtt acccttcgcg ttgcacagct tgaagccgtt ttcaccgctc ttgaaccgtt
     1801 gaggctcccg cccttcatcg gttcgaccct ccgcggtgcg ttcgggcatg ccttgaagag
     1861 cgcggtgtgc gtgatgcggg cacgcgagtg ccattcctgc cagctttccc gcggctgcct
     1921 ttatacccga attttcgaaa cagacccgga gggtgcggac cttcgtcacc tcggcgtgac
     1981 ggacaacgcg ccccggccgt acgtgtttgc ccccggaatg ggcacaccgg agatactcca
     2041 gcccggcgac acgctttccc ttaccatgcg acttttcggc tgggcggtgg actactcgtc
     2101 ctatgtcatg tatgcgctgt cactgcttgc ggaacgcgga ctcggtgcag cgaggggaag
     2161 gctccagctc acccgtgtta ttgacggcac tacgggcctc gcggcatacg actgcgagga
     2221 cggggacgtc tacgagccga tcaagttcgt ccgcctgcct gtgcccattc ctgccgccgg
     2281 gcagggagac gtcaccgttc tctggcagac gccgctccgg gtggtgaacg cgcaccagct
     2341 cgtccaggaa ccgaccgctc ctgtggtcat gcgcacgctg ctccgccggg tttcggggct
     2401 gctcacgtac cacgaaaacg ccgcgctgcc ctacaacgcg caggaggtgc tggcggcaac
     2461 ggaccgggtg gcggtgaagc ggaactccac gaactggtat gactggaagc ggtacagcaa
     2521 ccggcagaac cgcgcggttg agatgggcgg tattcttggg aaaacgacgt atgaggacac
     2581 agaaggcgtg ctgcggccat ggctgacgct ggcagagacg atacatctgg ggaagagctg
     2641 cacgttcggg ctgggcgccg tccgaatcgt tgcgtaggag ggatccgatg acggataagg
     2701 aagcagttgt tctgggcgcg cttcttcacg acgtgggcaa gttctggcag cgcacgtccg
     2761 ccagacgtgt ggacacgggg atggatgcgt acgtgtgccc accgggcgga actcacgttc
     2821 acgcggtgtg gacgtaccgc tttttcgagg aactcggcgc gggcacgccg ggcagtacag
     2881 ggcagtggct tggccttgcc cgggatgcgg cggcattcca tcacaacccg cgtggcgaca
//
LOCUS       DAOSOO010000308         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_34923_length_2863_cov_4.596866, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000308 DAOSOO010000000
VERSION     DAOSOO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34923_length_2863_cov_4.596866"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            843..>2863
                     /locus_tag="PLT86_14235"
     CDS             843..>2863
                     /locus_tag="PLT86_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOT37816.1"
                     /translation="MHSNIEALIEEHAARMAEGMLAAARSARSEEDVRHAVNTLIDAF
                     VKHAGLNIPGRHEYGLAGGRIDSKYGRVIIEYKDPKGSGKITLNKDAPGNRAVVEQLT
                     KRFHDLQVEEHVETERILGVGCDGDTFVFVKMPGRDAVVDEPQPVTAYSVMRLLRAIV
                     SLGARGLSLTPKNLAERFGSDGESARKGIRLIHDLIHETQNPKAQIFFRQWQILFGEV
                     CGYDIHGRNPKIAKLAEHYGVREADPAKLLFAVHTYYALFIKLLAAEIVASFSPLGTS
                     TLEQIASAATPEALRERLRTLEQGGIWSQLGIHNFLEGDLFSWYLDAWDERSEQAVRG
                     MTTALKEFDATTLSVEPSESRDLLKQLYQQLFPKSVRHDLGEYYTPDWLAELVLDELG
                     YDGNPDRRLLDPACGSGTFLVMALNRVKAWYAQHRYECGYDEKELLRRVLNNIIGFDL
                     NPLAVMAARTNYLMAIRDLVRHADSVELPVYLCDSIMTPKDFSTAAAAFDYVPVTTAV
                     GQFNIPNEVLKEREKFHTYARTLHQCIENDYTFGEFAQRCAAEGVPTTQHEHHRKVYD
                     SLLEMKRQDRNGIWARIIKNAFAPLFVGTVDFVAGNPPWVNWENLPEEYRNKMKPLWQ
                     EYGLFTLSGSAGRLGGGKKDLSMLFVYSGVDRFLRNGGKLGFVITQSVF"
BASE COUNT          594 a          891 c          851 g          527 t
ORIGIN      
        1 ttttttcggg aggcgaagaa atggcgcacg cgaaggcgcg aagggcgcga agaaatgggc
       61 gttttttgtc gtaggggcaa ttcatgaatt gcccctgcaa ccccggttgg gcgcgggtcc
      121 ctgcaccacg gccgcagatt atggagggac ccgctgagaa ggcggtgcgt tcttcgtcgt
      181 atccattttc cgccgcggcc gggtttttcg aaaggcacga aaggagcttg cctcgcatac
      241 cggttgaggc aaaacgtcgt cttcgtgtca agtgtttttg cgaagaagtt gcaaatttgc
      301 accaggtttt ttcgggaggc gaagaaatgg cgcacgcgaa ggcgcgaagg gcgcgaagaa
      361 atgggcgttt tttgtcgtag gggcaattca tgaattgccc ctgcaacccc ggttgggcgc
      421 gggtccctgc accacggccg cagattatgg agggacccgc tgagaacgcc gtgcgttctt
      481 cgtcgtatcc attttccgcc gcaaacggca cgaggccggc cttcctccct catccctgct
      541 ccgctgcgcg gagccactgc cgcgtcgagc gcggcttccc ttcccccggt ggtggaaggg
      601 agcgcgccgt tttcccctct accaccggga gaggggaaac ctccgcggag gcggaggagg
      661 ggtgagggaa cacgccccca cccaaccccc acaaaaccgc ccccgccgtt ccaccccctt
      721 gacaatcccg tctccccgcg ccacattcgc acccatgtca acgctcggtt cacgaccccg
      781 ccattgaccg gcggggcatc gtcggtcaaa taacaacccg ccaaatacgg aggcggtgcc
      841 gcatgcactc caatatcgaa gctctcatcg aggaacatgc cgcccgtatg gcggaaggca
      901 tgctcgccgc ggcgagaagc gcccgttcgg aggaagacgt tcggcacgcg gtcaacaccc
      961 tcattgacgc atttgtgaaa cacgccgggc tgaacatccc cggacgccac gagtacggtc
     1021 tggccggcgg gcgcatcgac tccaaatacg gccgcgtcat catcgaatac aaagacccga
     1081 aaggttccgg caagatcacc ctgaacaagg acgcacccgg caacagggcg gtcgtggaac
     1141 agcttacgaa gcgcttccac gatcttcagg ttgaagagca cgtggaaacg gaacgcatcc
     1201 ttggcgtcgg gtgcgacggc gacacgttcg tgttcgtgaa gatgcccggc agagacgcgg
     1261 ttgttgacga accgcagcct gtgacggcgt attcagtgat gcggttgctc cgcgccatcg
     1321 tttcgttggg cgcgcgcggg ttgtccttaa cgccgaagaa ccttgcggaa cgtttcggat
     1381 cagatggaga aagcgcgcgg aagggcatcc gcctcatcca cgaccttatc cacgaaacgc
     1441 agaaccccaa agcgcagatc ttcttccgcc agtggcagat tctgttcggc gaggtgtgcg
     1501 ggtacgacat ccacggccgg aatcccaaaa tcgcgaaact tgcggagcat tacggcgtgc
     1561 gggaggccga ccccgccaaa ctcctgttcg cggttcacac ctattacgct ctgttcatca
     1621 aactgctcgc cgcggaaatc gtggcgtcat tctctccact tggcacctcg actctggaac
     1681 agatcgccag cgcggccact cccgaggcgc ttcgcgaacg gctgcgcact ctcgaacagg
     1741 gcggcatctg gtcgcaactc ggcatccaca acttccttga gggcgacctc ttctcgtggt
     1801 atctcgacgc gtgggacgaa cgcagcgaac aggccgtcag gggaatgact accgcgctca
     1861 aggagttcga cgcgaccacg ctcagcgtcg aaccatccga atcgcgcgac ctgctcaagc
     1921 agctttacca gcagcttttc ccaaaatctg tccggcacga cctcggggaa tactacaccc
     1981 ctgattggct cgccgaactg gtccttgacg aactcgggta cgacggtaac cccgacaggc
     2041 gcctgctcga ccccgcctgc ggttccggca ccttccttgt gatggcgctc aaccgtgtga
     2101 aggcgtggta tgctcagcac cggtacgagt gcggctacga cgagaaggaa ctgcttcgca
     2161 gagtgctgaa caacatcatc gggttcgacc tgaatccgct ggccgtcatg gcggcgcgca
     2221 ccaactacct gatggcaatc cgcgacctgg tgcgacatgc cgacagcgtc gagctgcccg
     2281 tatatctgtg cgattccatc atgacgccga aagatttcag taccgcggcg gcggcgttcg
     2341 attatgtgcc ggtgactacc gccgtcgggc agttcaacat tcccaacgaa gtgttgaaag
     2401 aacgggagaa gttccacacc tacgcgcgaa ccctgcacca gtgcattgaa aacgactaca
     2461 ccttcggcga gttcgcgcaa cgctgcgccg ctgagggtgt tcccaccacc caacacgagc
     2521 accacaggaa ggtgtatgat tcgctgctgg agatgaaacg gcaggatcga aacggcatct
     2581 gggcgcgcat catcaagaac gcctttgcgc cgctcttcgt cggaaccgtg gacttcgtgg
     2641 cgggcaaccc gccgtgggtc aactgggaaa acctgccgga agagtaccgc aacaagatga
     2701 agccgctctg gcaggagtat ggcctgttca cgctttcggg ttccgcagga cgcctcggcg
     2761 gaggcaagaa agacctttcg atgctgttcg tctactccgg ggtcgaccgg ttccttcgaa
     2821 acggcgggaa gctggggttc gtcatcaccc agagcgtgtt caa
//
LOCUS       DAOSOO010000309         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_35073_length_2854_cov_5.092176, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000309 DAOSOO010000000
VERSION     DAOSOO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35073_length_2854_cov_5.092176"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..2409)
                     /locus_tag="PLT86_14240"
     CDS             complement(421..2409)
                     /locus_tag="PLT86_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT37817.1"
                     /translation="MRTQFDLSDLSWKLAGFMPYGWHCANYPAIVPTPKIGVAGIEVE
                     AIPARVPGSVQGALREAGVIPDWNEGLNSRQSEWVESRHWVYETFLPDDWLAKGRSFR
                     LVCLGLDYAGWVVLNGRVIGEFRGSLVPHMFDFTGLLAPSSNRLQIAFDCPPRWLGQL
                     GYTSRMKEWKPRFNYGWDWTCRLVQIGIWDDIRLEVTDGPVLEDVRCFTGYDMQSRLG
                     SVTVFAATSADAAGTVRLTLKRGDLVVARNEVPLADARGGYVWKGLPVEPWYPNGHGE
                     HPVYTLVVELVGSDGSVLDAETRHVGFKHVEWRRCEGAPEHADPWICVVNGVPVFLQG
                     VNWSPIRPNFADLAETDYRTRLEHYRDMGCTIIRVWGGGFLEKPWFHDMCDEMGLMVW
                     GEFPLSSSGLDNWPPEDEQSIASLAEIAESYIRRRQHHVSLLLWCGGNELYGGLDGGK
                     VGSEVPVTTAHPLIRRLAEVVKQFDPTRRFLPASPSGPISLMRDEDIGKGLHWDMHGP
                     WKPEGRLSGSWTELWAKHDAYIHSEIGSPGASSVEIIRRYRGDLPDFPASWESALWRR
                     TSWWIEWDACVDELGREPADLEEYVAWSQARQREALTIAARSCKARFPRCGGFIVWHG
                     HDSFPCTANNALLDFHGDPKPAAYGLAEVFHARPEELR"
BASE COUNT          535 a         1015 c          840 g          464 t
ORIGIN      
        1 gtcgcgcgcc actcgacgac cgggttctcc gttccgcgcc cgccctgggc aggcaggaat
       61 gcctgcccca ccaacaaatg ggcaacggag tggcgggaca ggctgccaaa tccggcaaac
      121 acgccttact gccatggtcc tgtgttcccc acctgtacca attccccacc ccgataacgt
      181 cattcccgcg caggcgggaa tccagaaacg gcaagggaag caccacgcct tgcggccctt
      241 caggcctcat ggcgccgcat gggctggtga agcgccaacg gcgcgcgata accgagcccg
      301 gttcgcagga ccgggcaggc gtgaccggcc ttcaccaatt ccccgcacca attgcgtcat
      361 tcccgcgaag gcgggaatcc agagcaaccc ttcggcacca acgcctgctt ccccgacgtg
      421 ctacctcagc tcttccggcc tcgcgtggaa cacctccgca aggccatacg ccgcgggctt
      481 cgggtcgccg tggaaatcca gcagcgcgtt gttcgcggtg caggggaacg aatcgtgccc
      541 gtgccagacg atgaagccgc cgcaccgggg gaaccgcgcc ttacaggagc gcgccgcgat
      601 ggtgagcgcc tcccgctgcc gcgcctggct ccacgcgacg tattcctcga ggtccgccgg
      661 ttcgcgtcca agttcatcca cgcaggcgtc ccattcgatc caccatgaag tgcgccgcca
      721 gagggcgctc tcccagcttg ccgggaaatc gggcaaatcg ccccggtaac gccggatgat
      781 ttcgacggag ctggcgcccg gcgagccgat ctcggagtga atgtaggcgt cgtgcttcgc
      841 ccacagctcc gtccagctcc cggagagccg cccttccggc ttccaggggc cgtgcatgtc
      901 ccagtggagc cccttcccga tgtcctcgtc gcgcatgagg gaaatcggcc cggaaggcga
      961 cgccggcagg aagcgcctcg tcgggtcgaa ttgcttcacc acctcggcga gccgccggat
     1021 gagcgggtga gcggtggtca ccggcacttc agacccgact tttccaccat cgagaccccc
     1081 gtacagctcg ttccctccgc accagagaag cagcgaaacg tggtgctggc gacgccggat
     1141 atacgactcg gcgatctccg cgagactggc gatgctctgt tcgtcctccg gcggccagtt
     1201 atcaagcccg gacgatgaaa gcgggaactc accccagacc ataagcccca tttcatcgca
     1261 catgtcgtgg aaccagggct tttcgaggaa tcctccgccc cagacgcgga tgatcgtgca
     1321 gcccatatcg cggtagtgct ccaggcgcgt gcggtaatcc gtttccgcga ggtcggcgaa
     1381 gtttggcctg atcgggctcc agttcacccc ctgaaggaac acaggaacgc cgttgacaac
     1441 gcagatccac gggtcggcat gttccggcgc accttcgcac cgccgccatt ccacatgttt
     1501 gaatcccacg tgccgcgttt cggcgtcgag caccgatccg tcgctgccaa ccaactcgac
     1561 gaccaaggtg tacaccgggt gttcgccgtg cccgttgggg taccatggct cgacgggcaa
     1621 gcctttccac acgtatccgc cgcgcgcgtc ggcaagtggc acctcgttcc gtgcgacaac
     1681 caagtcgccg cgtttcagcg tgagccgaac ggttcccgcc gcatcggcag acgtcgctgc
     1741 gaacaccgtt accgacccca gccggctctg catgtcgtac ccggtgaaac accgaacgtc
     1801 ctcgagaacc ggcccgtccg tcacttcgag gcggatgtca tcccagatgc cgatctgcac
     1861 gaggcgacag gtccagtccc agccatagtt gaaacgcggc ttccattctt tcatgcggga
     1921 ggtgtatccg agctgcccca gccacctcgg cgggcagtcg aacgcgatct gaaggcggtt
     1981 gctcgacgga gcaagcaggc cggtgaaatc aaacatgtgt ggcaccagcg aaccgcggaa
     2041 ctcgccgatg acgcggccgt tcagcaccac ccacccggcg tagtccagcc cgaggcacac
     2101 aagacggaac gacctgccct tcgccagcca gtcatccggc agaaacgtct cgtacaccca
     2161 gtggcggctc tccacccact cactctggcg ggaattgagc ccctcattcc agtccgggat
     2221 aacaccggct tcccgcaacg cgccctgcac cgaccccggc acccgcgcag ggattgcctc
     2281 cacctcgatt ccggcaacgc cgattttcgg ggtaggaacg attgccgggt agttcgcgca
     2341 gtgccatccg tacggcatga acccggcaag cttccagctc aggtcggaga ggtcgaactg
     2401 cgttctcact ggttacctcc agaaattgca ccgcggcgcc tctgcggact gaaacgacca
     2461 aggattcctg cgtacgcgga atccgtttgc cggctcgcat actcgatcct gcgttcccag
     2521 cccataccat tccccggcag cgataacgtc attcccgcgc aggcaggaat ccagaaacag
     2581 caagggaagc accacgcctc gcggcccttc aggcctcggg cagccgcatg gaccggtgaa
     2641 gcgccaacgg cgcgcgataa ccgagcccgg ttcgcaggac cgggcgggcg taacggccac
     2701 gccgcacctc acgttaaagt gcggcggccg gcccgcgtaa tccgaaagca cggcatttcg
     2761 gtcgtctcga tacatcgagc gccgtcacgg ggcgtcgctc gccactcgac gaccgggttc
     2821 tccgttccgc gcccgccctg ggcaggcaag aatg
//
LOCUS       DAOSOO010000310         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_35258_length_2842_cov_5.136706, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000310 DAOSOO010000000
VERSION     DAOSOO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35258_length_2842_cov_5.136706"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..2060
                     /locus_tag="PLT86_14245"
     CDS             312..2060
                     /locus_tag="PLT86_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37818.1"
                     /translation="MFTYENILDLVVRWAEDALPQQVLDPARDDDGAFIAGGWCLWEV
                     RGIPAPDVSNHAHAIACLAYAYLAVGSRYFGDAAMLDRTLRAIRFLRSAQRPTGLIDL
                     PSTNFESPPDTAFAVQLLAPVVEMARRVGTPESLRIAGDLGEFIRTGAAGIVGRGFHT
                     ANHRWVISAALAQATSLFPGLAPVAMPYFSRIMAEGIDLNEDGEYTERSTVVYNAVCD
                     RSLRFMADYLNRPDLLAFVRKNLDSMLRLLHADGTAVTSTSVREDRGRRIRPLSLADS
                     FYDMARRDKNGVWAHAADMLVGGDFAAEARAWPFDPVPGWLFGVYALHPEYRHDALGR
                     KPLPDEYSVFLPAAGLWRVRRGDLSATAASGHTTAFAVRYGDVSLKAVKLFGSYIVSA
                     IFSADEMAPDGQGEAVRGVSMRHRGDHRKLPGYDLPLNRPVPYGSFHDVAAERARWTQ
                     PPMDIVLDIHEVPGGFDLHARTEGGLDRIMFQIEFCFDGPGEWETDDALVAAENGQVV
                     FLKAGYGVFHRGRHGLRIGPGAISHRVPQMRNTEPEPDSFRVLMTFVTPVDHAFDIRY
                     GAWSMATGGLLASFER"
     gene            2168..2410
                     /locus_tag="PLT86_14250"
     CDS             2168..2410
                     /locus_tag="PLT86_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT37819.1"
                     /translation="MKVAVQKWGNSLALRIPRAFARDVGVTAGTVVAVSVADGRLIAE
                     PVRATRVRLTDLLRDVTPENLHGETATGPAVGVETW"
     gene            2410..2760
                     /gene="mazF"
                     /locus_tag="PLT86_14255"
     CDS             2410..2760
                     /gene="mazF"
                     /locus_tag="PLT86_14255"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HOT37820.1"
                     /translation="MAAGYIPGRGDIVWLTFSPQAGHEQAGRRPALVISPEAYNRVVG
                     LALFCPVTSQRKGYPFEVAIPDGLPVSGVVLSDQIKSLDWRARQAEFSARLPGDAMAE
                     VLAKVGTLLGDDED"
BASE COUNT          477 a          878 c          939 g          548 t
ORIGIN      
        1 gaagcgggac aacgcattct cggtgcgcag gtggtggagg cgatcctggc ggcgcggggc
       61 aggtgaacgg cgtcgcgttc ctcagacgct aaccgttcct gtgtcgccgt atatctccat
      121 cgtaagcgca ggtcgaaagt gggagtgatg aagggcgtga ccaaaaaatg ggctctcctt
      181 cgcatgccgt gtgcggccgc ccatcgcatg ggtctgactg cagcggtcgg aaaggtggct
      241 ccggttgcgg atggcgcaca gctggaaatc ctgacacacc ggacaggcgc ctgaagggag
      301 atggtcgctc gatgttcacg tacgaaaaca ttctcgatct cgtagtgagg tgggcggagg
      361 acgcgctccc gcagcaggtt ctcgatcccg cgcgcgacga cgatggcgca ttcatcgccg
      421 gcgggtggtg cctgtgggag gttcgcggta ttccagcgcc cgatgtctcc aaccacgccc
      481 acgcaatcgc ttgcctcgca tacgcgtacc tcgccgtggg ttcccgttac ttcggcgatg
      541 cggccatgct cgaccgtacg ctccgcgcga tccgcttcct caggtctgcg cagcggccga
      601 ccgggctcat cgacctcccg tcgacgaact tcgaatcgcc acccgacacg gcattcgcgg
      661 ttcaattact cgccccggtc gttgagatgg cgcggcgggt tggcacgccg gaatcgcttc
      721 gcatcgcggg cgatctcggg gagtttatcc gaacgggagc ggcaggcatc gtaggccgcg
      781 gattccacac tgccaaccac cgatgggtga tctcggccgc actcgctcag gcaacgtcgc
      841 tcttcccggg cctcgctccg gtggcgatgc cgtacttttc ccgcatcatg gcagagggga
      901 tcgacctgaa cgaagacggg gagtacaccg agcggagcac ggtcgtctac aatgcagtgt
      961 gcgatcggtc cctccggttc atggccgact acctcaaccg gccggacctc ctcgcctttg
     1021 tgcggaagaa tctcgactcg atgctccggc tcctccatgc agacggcacc gcggtgacaa
     1081 gcacctcagt ccgtgaagat cggggccggc gcatccgtcc gctctcgctt gccgactcgt
     1141 tctacgacat ggcccggcgc gataagaacg gcgtctgggc gcacgccgcc gacatgttgg
     1201 tcggaggtga tttcgctgca gaggcgcgag cgtggccatt tgatccggtc ccaggctggc
     1261 tcttcggtgt ctatgcgctc catcccgagt accggcatga cgcgctcgga cggaaaccgt
     1321 tgccggacga gtattcggtc ttcctgcccg cagcggggct ctggcgcgtg cggcgcggtg
     1381 atctttccgc gacggcggcc tccggccata cgacagcgtt tgcggtacgg tatggcgacg
     1441 tgtcgttgaa agcagtcaaa ctgttcggca gttacattgt ctcggcgatc ttctcggcgg
     1501 acgaaatggc gccggatggg cagggagagg ccgtccgcgg ggtttcgatg cgccacaggg
     1561 gcgaccaccg caaattgccc gggtatgacc tgccgctcaa caggcccgtg ccgtacggga
     1621 gcttccacga tgtcgccgct gagcgggcac gctggacgca gccgccgatg gacatcgtgc
     1681 tcgacatcca cgaggtgccg ggcggattcg acctccacgc tcgcaccgag gggggactcg
     1741 atcggatcat gttccagatt gagttctgtt ttgacggccc gggcgagtgg gaaaccgacg
     1801 acgcactcgt cgccgcggag aacgggcagg ttgtgttcct taaggcgggg tacggcgtgt
     1861 tccatcgagg gaggcacggc ttgcgcatcg gccccggcgc gatctcgcac cgcgtaccgc
     1921 aaatgcggaa caccgagccg gagcctgatt ccttccgtgt gcttatgact ttcgtcacac
     1981 ctgttgatca tgcgtttgat atccggtacg gcgcatggtc gatggccaca ggggggcttt
     2041 tggcgtcgtt cgagcggtga ttccggcccc tccgttcatc ctgttgattt gttgaatttc
     2101 ccgtacggtt gacgacctct gcgcaaaaat gtatcttcat cgtatctaca cgcgagcagg
     2161 aggaacgatg aaagttgcag tgcagaaatg gggcaacagc ctcgctctcc gtataccccg
     2221 cgcgttcgca cgtgacgtcg gtgtgacggc aggaaccgtg gtggcagttt ccgtcgccga
     2281 tggcaggctg atcgccgaac cggtccgcgc caccagggtc agactcaccg atcttctccg
     2341 cgacgtgaca cccgaaaacc tgcacggcga aacagcgacc ggccccgcgg tcggcgtgga
     2401 gacgtggtag tggcggccgg atatatcccc ggccgcgggg atatcgtctg gctgacgttt
     2461 tccccccaag cgggccacga acaggccgga cgccgccccg ctctggttat ttcgcccgag
     2521 gcgtacaacc gcgtggttgg gctggcgttg ttctgccccg tcaccagcca gaggaagggc
     2581 tacccgttcg aggttgccat acccgacggc ttgcccgtca gcggcgttgt gctgtcggac
     2641 cagatcaaaa gcctggactg gcgggctcgt caggcggagt tctccgcgcg cttgccgggc
     2701 gatgcgatgg cagaggtgct ggcgaaagtc gggacgctgc ttggcgacga cgaggactga
     2761 tttcgcaacg cagaatgaga ggccccggtg gattggttga gcccgcaagt ctggcggcgt
     2821 gattctgacg ttccctgcgt gt
//
LOCUS       DAOSOO010000311         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_35373_length_2835_cov_4.979137, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000311 DAOSOO010000000
VERSION     DAOSOO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35373_length_2835_cov_4.979137"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1314)
                     /locus_tag="PLT86_14260"
     CDS             complement(247..1314)
                     /locus_tag="PLT86_14260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37821.1"
                     /translation="MEHSPLRVAIVGQGRSGYDIHVARLRNDSRFRIVAVADFDKARR
                     EQSARELGCKTYRTHAEMLRRTDAELVIVSSYSFSHAPIACQALEAGKHVLVEKPIAM
                     NTRWVDRMIRARDQSGKRLFPFHNYRYTKEYLHLKEMIASGILGDVFEIRMRALAFSR
                     RNDWQTTRLYGGGVLNNTCPHFLDLLLQMLQSPVAEVFTDLKLVQNVGDVENHVRVII
                     KGRNGRIADMLVSSADAFPEPKWTILGSNGTLICDGKTSELRYFDPGDLQKGYGVRRG
                     PSPNRSYDFADSIPWKQKTVAAESHVPHDLHDNVFAVLRENAAQDITVEDVRDVVAIT
                     ERARRKDGFYGGVSRAYNPER"
     gene            complement(1335..2342)
                     /locus_tag="PLT86_14265"
     CDS             complement(1335..2342)
                     /locus_tag="PLT86_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT37822.1"
                     /translation="MAHLTVRRWWLWITVLLVAVVFVAVISVGLGSTWVPASDVLAAF
                     GSSRGVNAVIVREVRLPRVLLAGVVGGGLAVAGVVFQALLRNSLADPFVLGISGGAGL
                     GAVIAVSIGGGVSIAGLAGVPLFAFFGALIAIIVVYLFARGGGRVNLHTMLLAGVVTN
                     SVCSSAIMALTITLSYDQLQTVMFWLMGHLSTMPLPLVGKLALYVGIGLVFLMAIGRD
                     LNQMSLGESQAATLGTNVESVKRIAFVGASLVTGASVAAAGLIGFVGLIIPHAVRIST
                     GPDHRLLIPASFLAGAAFLMLADALARTVIAPTELPVGVITALIGGPVFLVLLKQRMK
                     R"
     gene            complement(2646..>2835)
                     /locus_tag="PLT86_14270"
     CDS             complement(2646..>2835)
                     /locus_tag="PLT86_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37823.1"
                     /translation="KEYLAAHVQRPITIADAVEAKGEDFPEAAEGATEEPVDAVHDDE
                     PADREDATPASSDEQPEG"
BASE COUNT          649 a          878 c          755 g          553 t
ORIGIN      
        1 tccgtaatcg tgcagttcat cgtgcgtaac gagcatagaa cgtaccgaag gaaaatgaaa
       61 gaaggcgcca gcgcgcggtt gcaggaactg gcgccgggaa agcgcggatt atcaacggct
      121 ttcccattta cgacaaatga aagttaccca acaacatcac acgggtcgag tcctgcgcag
      181 cggatttgcc gcaacgagtc gctccgttcc tcggattggc gggcaattcc ggcttcggga
      241 agagagttaa cgctcggggt tgtacgcccg ggatactccg ccatagaacc cgtccttccg
      301 cctcgctcgc tcggtgatcg ctaccacgtc gcggacatcc tcgaccgtga tatcctgtgc
      361 ggcgttttcc cgcagaacag caaacacatt gtcatggaga tcgtggggaa cgtgtgattc
      421 ggccgcgaca gttttctgct tccacggaat tgagtccgcg aaatcgtaac tgcggttggg
      481 ggagggccct cgcctgacgc cgtacccttt ctgcaagtcc ccggggtcga agtaacggag
      541 ttcgcttgtt tttccgtcgc agatcagggt gccattgctt ccgagtatgg tccatttggg
      601 ctcaggaaag gcgtccgcgg aagaaacgag catgtccgcg atacgcccat ttctgccttt
      661 aataatgacc ctgacgtggt tctcgacgtc tccgacattc tggacgagtt tgaggtccgt
      721 aaacacctct gccaccgggg attgcagcat ttgcagaaga aggtcgagga aatgcgggca
      781 cgtgttgttg agtacccctc cgccgtacag tctggtcgtc tgccagtcgt ttcggcgcga
      841 gaaagccaga gcgcgcatcc gaatctcgaa cacatccccc agtatgccgg aagcaatcat
      901 ctccttcaga tgaaggtatt ccttcgtgta acggtagttg tggaaaggga acagacgctt
      961 gcccgattga tcccgcgcgc gaatcatcct gtccacccag cgggtattca tcgcaatcgg
     1021 cttttccacc aacacgtgtt tgcccgcctc gagcgcctgg catgcgatcg gagcgtgtga
     1081 aaacgagtaa gaagacacaa tgaccaactc ggcatcggtt cgtcgcaaca tttcggcgtg
     1141 agtgcggtat gttttgcatc cgagttctcg tgcgctttgt tcccgacgag ctttgtcgaa
     1201 gtccgcaacc gcgacaattc tgaaccttga atcgttcctc aatcgagcga cgtgaatgtc
     1261 gtaaccagaa cgcccttgac ccactatggc gacgcgcaga ggcgagtgtt ccatttcctc
     1321 ttcccttccg atagttaccg tttcatccgt tgcttgagga gtacgagaaa cacggggccg
     1381 ccgatcagcg cggtaataac gcccacaggc aactccgtcg gtgctatcac ggtccgcgcc
     1441 agcgcgtctg ccagcatcag aaacgcagca cctgcgagaa aactggccgg aatgagcaaa
     1501 cgatgatccg ggccggtcga aatccgtacc gcgtgtggaa taatcaaacc gacaaacccg
     1561 attaaccccg ctgccgccac cgaagcaccc gtcaccagtg aggcgccgac aaacgcgatc
     1621 cgtttcaccg attccacgtt tgtccccagc gttgcagcct gtgactcacc cagactcatc
     1681 tgattcaaat cgcgcccgat tgccatgaga aaaacgagcc cgattcccac gtaaagagca
     1741 agttttccta cgagcggaag cggcatcgtg ctcagatgcc ccatcaacca gaacatcact
     1801 gtctgcaact ggtcgtacga tagagttatc gtcagcgcca tgatggcaga agaacagacg
     1861 gaatttgtga ccacccccgc cagcagcatc gtatggaggt taacccggcc accgccccgg
     1921 gcgaacaggt agaccacgat gatcgcgatc agcgcaccaa agaaggcaaa cagcggcaca
     1981 ccggccaacc cggcgatact cacgccaccc ccgatggata ccgcgatcac cgcgcccagt
     2041 cctgcgccgc ccgatatgcc cagcacgaac ggatcggcaa gtgagttccg aagaagcgcc
     2101 tgaaacacga caccggcaac cgcaaggcct cctccaacca ccccggcgag cagcacacgg
     2161 ggaagccgga cttcccgcac gataacggcg ttgacgcctc tcgaagagcc gaacgctgcc
     2221 agaacgtcgc tcgcgggaac ccatgtagac cccaggccca ccgaaatcac cgcgacgaac
     2281 accacggcaa ccagaagaac ggtaatccag agccaccagc gacgcaccgt caggtgagcc
     2341 aattcaggta cctccaaata gactgaggtc aggctgacgc aaacacttct gccgggacct
     2401 tggatccctt gttcggaagt ggacccgggc ccttgcgggt tgtatcaaaa ttcctgggaa
     2461 gaacgacttc tattttcggc aaacagactc gcggtccggg gagaaatgct cccggtttgc
     2521 gacactcccc agatatatgc gcaatatcgc agctatacca gccacagggc acaaatggtc
     2581 agggggtgtt cgaagcaccc cctgacctca tggcacacgc gttcgtgaca aaccacttgt
     2641 tcctgctacc cttcgggttg ttcatccgac gaggcaggcg ttgcatcttc cctgtctgcc
     2701 ggttcgtcat cgtgaacagc atccacaggc tcttccgtcg caccctcggc agcttcaggg
     2761 aaatcctctc ccttcgcttc cactgcgtcc gcaattgtga tcgggcgttg cacatgagcc
     2821 gcgaggtact ccttc
//
LOCUS       DAOSOO010000312         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_35553_length_2823_cov_3.989162, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000312 DAOSOO010000000
VERSION     DAOSOO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35553_length_2823_cov_3.989162"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PLT86_14275"
     CDS             complement(<1..1257)
                     /locus_tag="PLT86_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37824.1"
                     /translation="MWNWNRDFRAWNHFYHWNQEELNWPVFASGHPELALGYLKMRAK
                     ALPLAQQSAMRKYGIDGAWYSDVFERRGWQDEGVPLFTPGLQIALDMWRYWRYTGDEQ
                     FLAETAYPVMREACRFYLGMLKEESDGLFHLSASHPYEHASGYMVRDCLTDMAHIRTA
                     FPATAAVAERLGEREFAERLRDVAARLPRVETLPLPERRYRDTPGGPEFTDGWAKGLK
                     CPSDRILCVGRRVEDGRPVHFRAVEEGTGSDHLFPGSDESLVFPSGALGLRDKGSELF
                     EAARTTALCNWEEIMGWSVTPIVFARLGMSDLAEEALRRHIIHFQHFPQGLWNYQSSQ
                     SGSATDFATTFRVHDRENGRDEFPFLRQPHTHFGLEPGAVVQTTVNEMFLQSYDGVIR
                     VCPSVPQDWDGAFSLWAEGGFRISARV"
     gene            complement(1291..2241)
                     /locus_tag="PLT86_14280"
     CDS             complement(1291..2241)
                     /locus_tag="PLT86_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37825.1"
                     /translation="MQHSVASFGRDLLQVDYASFLSQHDVVYETPPAIGCHGLPIGNG
                     VSGALVWTPPTAVRWELGGSDLWADGPARPFGSWNKEDEEVATALLSAGSLSIGYDIP
                     AFDWAYLKKFSARLSLFPAEVSLNAETPFSRVRINTWMNSDPSCLVIQCSDDLDDAIP
                     RKITLERWGSRVFAHWYRTIVRDPSRGLGGTEASVHGDVIILRHSSRSHDFVVAAKVT
                     GVPSVARAAHRHAAVADVAAVRSAEFTVLLTIASSETTGDPLQTALERIANAEAAGVS
                     NLQTGHRRWWAGFWERSFVNIPDAYLENLWYINQYVMGSS"
     gene            2363..>2823
                     /locus_tag="PLT86_14285"
     CDS             2363..>2823
                     /locus_tag="PLT86_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT37826.1"
                     /translation="MPAFAGRTALVTGASKGIGYHIARLCAREGADVILVARDVARLE
                     RAAEEITGSFPVKAAVFPADLSKPDSAAELWHRVQSAGHSVDILVNNAGIGAYGEFHE
                     ISPEKTRGMRHLNIVSFTELMHLAAVEMARRKWGRILTVASVAGFQAMPYMA"
BASE COUNT          622 a          904 c          774 g          523 t
ORIGIN      
        1 aactcgcgcg gaaatgcgaa accctccttc cgcccatagc gaaaacgccc cgtcccaatc
       61 ctgcggaacc gacggacaca cccggataac gccatcgtag ctctgcagaa acatctcatt
      121 gaccgtagtc tgaaccaccg ctcccggttc aagcccgaaa tgggtatggg gttgccggag
      181 gaacgggaac tcatcgcgcc cattctcccg atcatgcaca cggaaggtcg tggcaaaatc
      241 ggtcgccgaa ccggattgac tgctctggta gttccacagc ccctgcggga agtgctgaaa
      301 atggataatg tgccttcgga gcgcctcctc agccaggtcg gacatcccca gcctcgcaaa
      361 cacgatcggg gtcacgctcc accccatgat ttcctcccag ttgcagagcg cggttgtacg
      421 ggccgcctcg aaaagttcgc tccctttgtc tctgagcccg agcgctccgg atgggaacac
      481 cagcgactca tccgaccccg gaaacagatg gtccgaaccc gtcccttctt ccactgcgcg
      541 aaaatgcacc ggacgaccgt cttccaccct gcgtcccacg cacagaatcc tgtccgaggg
      601 gcatttcagc cccttcgccc acccatcagt gaattccgga cccccgggag tgtctcggta
      661 gcgtctttcg ggtaacggca gcgtttcaac gcgcggaagt cgcgccgcga cgtcccgaag
      721 ccgctccgcg aattccctct caccaaggcg ctccgctacg gccgcagttg ccggaaatgc
      781 ggtacgtatg tgtgccatgt ctgtcaggca atcacgcacc atgtacccgc ttgcgtgttc
      841 atacggatga ctcgccgaaa ggtgaaacaa cccatccgac tcctccttca acatacccag
      901 atagaacctg cacgcttcgc gcatcactgg gtacgccgtc tctgcgagga actgctcatc
      961 gccggtatac cgccagtacc gccacatgtc cagcgcaatc tgaaggcccg gcgtgaacaa
     1021 cggcacgcct tcatcctgcc agccacgacg ctcaaacacg tcgctgtacc acgcgccgtc
     1081 gattccgtac ttccgcattg cgctctgttg tgccagcgga agggcctttg cacgcatttt
     1141 cagatacccg agcgccagtt cgggatgccc cgatgcgaaa accggccagt tcagctcttc
     1201 ctgattccag tggtaaaagt gattccacgc gcggaaatcg cgattccagt tccagattcc
     1261 gttgatgaaa tgaggcgggt aatccccctg ctaactcgag cccatcacgt actgattgat
     1321 gtaccagagg ttttccagat acgcatcagg gatgttcacg aacgagcgct cccagaaacc
     1381 cgcccaccac cggcggtgcc cggtctggag attcgagact cccgccgctt ccgcattcgc
     1441 gatccgttcc agcgcagtct gcagagggtc tccggtggtc tcgctggagg caatcgtaag
     1501 caggacggtg aattccgccg aacgaaccgc cgcaacatcc gccacagcag catggcggtg
     1561 cgctgcccgt gcaaccgaag gaacacctgt tacttttgcc gcaacaacaa agtcatgcga
     1621 gcgggagctg tgccgaagaa taatcacgtc gccgtgcacg ctggcctcgg ttccaccgag
     1681 acctcgtgac ggatcccgaa cgatggtacg gtaccaatgc gcgaacacac ggcttcccca
     1741 ccgttccagc gtgatctttc gagggatcgc gtcgtccaga tcgtcggaac actgtatgac
     1801 caagcaggaa ggatcgctgt tcatccacgt gttgatgcga actcgtgaaa acggcgtttc
     1861 cgcgttgaga gacacctcag ccggaaaaag agagaggcgg gcggagaact tcttcagata
     1921 cgcccagtcg aacgcaggga tgtcgtaacc gatcgagagt gaacccgccg agagcagagc
     1981 agtagcaacc tcctcgtcct ctttgttcca tgatccaaag ggtcgcgcgg gaccgtctgc
     2041 ccagagatct gaaccgccca gctcccagcg taccgccgtc ggcggggtcc acaccagtgc
     2101 gcccgaaacc ccgtttccga tgggcagccc gtggcatccg atcgccggtg gcgtttcata
     2161 caccacatca tgctggctga gaaaagaggc gtaatccacc tgaagaagat ctctgccgaa
     2221 cgacgcgacg ctgtgttgca tgtacccgaa tctccattgg ggggtgataa ccattcacgc
     2281 gggggaactc gctgggagaa aaaacacgaa attaagaata cctgaaatcg gctccgggtg
     2341 gaatccgcga caaggagatc gcatgccggc attcgcaggc agaacagcgc tcgtgacggg
     2401 cgcttcaaag ggaatcggtt accacatcgc aaggctttgc gcgcgtgaag gggcggacgt
     2461 aatcctggtc gcgagggacg ttgcacgtct cgaacgcgcg gcagaagaga ttaccgggag
     2521 cttccccgtc aaagcggcag tgttccccgc cgacctcagc aaaccagact ccgcagcaga
     2581 actgtggcat cgggttcaat cagcgggaca cagtgttgac attctcgtca acaatgccgg
     2641 cataggcgca tacggtgagt tccatgagat ctcgccggaa aaaacacgcg gaatgcggca
     2701 cctgaatatc gtttcgttca ccgagctcat gcaccttgcg gccgtcgaga tggcccgtcg
     2761 gaaatggggc cgcatcctca ccgttgcctc tgtcgcgggt tttcaggcca tgccgtacat
     2821 ggc
//
LOCUS       DAOSOO010000313         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_35857_length_2805_cov_5.500000, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000313 DAOSOO010000000
VERSION     DAOSOO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35857_length_2805_cov_5.500000"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1692)
                     /gene="ilvD"
                     /locus_tag="PLT86_14290"
     CDS             complement(16..1692)
                     /gene="ilvD"
                     /locus_tag="PLT86_14290"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT37827.1"
                     /translation="MVLNKYSRRITEPLSQAASQAMLYAIGLKEEDLSKPQVGIASMG
                     YDGNPCNMHLNKLAGYAKESVWENGMVGLVFHTIGVSDGIAMGTDGMNYSLQSREIIA
                     DSIQTVVAAHWYDAVIALPGCDKNMPGSIIAMCRLNRPALMIYGGTIAPGYWNGKKLD
                     IVSSFESFGQYLSGQITKEELKNILQHACPGAGACGGMYTANTVASAIETLGMTVPGS
                     SSRPAESPEKTQECRTIGATVRNLIEKDIKPRDIITRKALENAITMVMALGGSTNAVL
                     HLIAIAKAAGVPLTVDDFQRISDRTPYIADLKPSGKYVMEELHTVGGVPAVQKLLLRE
                     GYLDGSCLTVTGKTLAENLAEADDLKPGQTVIMPLSNPIKKTGHIQILYGNLAPGSAV
                     AKITGKEGEVFSGPAKVYDGEEETLRALERDEVKPGDVVVIRYEGPKGGPGMSEMLTV
                     TATIMGKGLGSSVALITDGRFSGGTHGFVVGHVTPEAQDGGPIALVRTGDKITIDARS
                     RQLIVHISDDEMNARRNAWVAPPYKATNGTLRKFIENVSPASEGCVTDQF"
     gene            2028..>2805
                     /locus_tag="PLT86_14295"
     CDS             2028..>2805
                     /locus_tag="PLT86_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37828.1"
                     /translation="MPRRQMDIPITHVHHGAYTLEGDIEPIIAELRPAPPVLYQCSST
                     ATPLPNTAGAAIATPTDPDRFCSPHEIRERLNLARKWVSELHGIGIRWVIPYTCNVKL
                     GGKYEGRKGIWKFYDHWDDYAFLGIGPRPEADPIEWMARERDGQLHYSYEKRHPGFTS
                     CGWYKYTPCLNNPHFNTYQRANVRTIALAGYDGVFVDNNNINCYCQHCEKLFKDWMSK
                     RHTLDQLHERFGWSTYDEVHLGWRGNRMDWVKYDPLFRRFV"
BASE COUNT          572 a          854 c          749 g          630 t
ORIGIN      
        1 ggtcaagtcc tgctctcaga actgatccgt cacgcagcct tctgatgcgg gcgaaacgtt
       61 ttcgatgaat ttgcgcagtg tgccattggt tgccttgtaa ggcggggcaa cccaggcgtt
      121 cctacgagca ttcatctcat catcggagat gtgcacgatg agctgcctcg aacgtgcgtc
      181 aatggtgatt ttgtcaccgg tccgaaccag cgcgataggt ccgccgtcct gtgcttccgg
      241 tgtcacatgc cccacgacga acccgtgcgt cccaccggag aagcgcccgt cggtgatgag
      301 agctacgctg cttccgagac cctttcccat gatcgtggcg gtaacggtga gcatttcgct
      361 cataccgggt ccgcctttcg gcccttcgta acggataacc acgacgtcgc ccggtttgac
      421 ctcgtccctt tccagagcac ggagagtctc ttcctctccg tcgtacactt ttgccgggcc
      481 gctgaacact tcgccctctt tgccggttat ctttgcgacc gcacttccgg gggcaaggtt
      541 gccgtagaga atctgtatgt gcccggtctt ctttatcggg ttactcaaag gcatgataac
      601 cgtctgcccc ggtttgagat cgtctgcctc ggcgagattc tcagcgagcg tctttccggt
      661 caccgtcagg caactgccat cgagatatcc ttctctcaga agaagcttct gcacggccgg
      721 aacgcctcca acggtatgca gttcttccat cacgtatttc ccgcttggtt tcagatccgc
      781 gatgtacggg gttctgtcgc ttatgcgctg gaagtcatct accgtcaacg gaacccccgc
      841 agccttcgca atggcgataa gatgcaggac ggcattcgtt gacccgccca gcgccatgac
      901 catcgtgatc gcattctcaa gggcttttct cgtgatgatg tccctcggct tgatatcctt
      961 ttcgatcagg ttcctgactg tcgcgcctat tgtccggcac tcctgcgttt tctcagggct
     1021 ctccgccggt ctcgatgaac tgcccggcac ggtcatcccc aacgtctcga tcgctgaggc
     1081 cacggtattc gcagtataca tccctccaca ggcacctgct ccagggcagg cgtgctggag
     1141 gatgtttttg agttcctctt tggtgatctg gccggagaga tactgcccga agctctcgaa
     1201 tgatgacaca atgtcgagct tcttaccatt ccagtatccc ggagctatcg ttccgccgta
     1261 aatcatcagg gcggggcgat taagcctgca catcgcgatg atcgagccgg gcatgttttt
     1321 gtcacatccc ggaagggcaa tgactgcgtc gtaccagtgc gcggcaacca ccgtctggat
     1381 ggaatcggcg atgatttccc gggactggag ggagtaattc ataccgtcgg ttcccatcgc
     1441 aatcccatcg ctcaccccga ttgtgtggaa cacaagcccg accatgccgt tttcccagac
     1501 tgattccttc gcgtatccgg cgagcttgtt aagatgcatg ttgcagggat tgccatcgta
     1561 tcccatgctc gcgataccaa cctgaggctt ggaaaggtct tcctccttga gcccgattgc
     1621 gtacaacatc gcctgagaag ctgcctgcga aagcggttcc gtaatgcgcc gactgtattt
     1681 gttcagtacc atagttttac ctcccgtagg gcattgagct ccacatgttg tgcctgccac
     1741 gcgcgaattc catgttgtac aaatatccgg ccgctatcca agctatcaaa gccggaaaag
     1801 cctccgagta tgtgcacgcg ctggtatgtc accggttctg aggcgtggaa cgccggtttg
     1861 ggaaatcctc ccaccatccc tatcattttc cagcgaaggc cccctcgcca tcactcgcgg
     1921 acgtcaaccg tccctgtccc gaccgctcaa aagggggcac aaacggcgcc acgaactctc
     1981 acaacctgga gcacctctct ccaaacccga ctatgacgga gacggaaatg ccgcgcaggc
     2041 aaatggatat accgataacc cacgtgcatc acggtgcgta cacgctcgag ggtgacattg
     2101 agccgatcat cgcggaactg agaccggcgc caccggttct ttaccagtgc agcagcacgg
     2161 caacgccgct gccaaacacc gcgggtgctg ccatcgccac tcccaccgac ccggacaggt
     2221 tttgttcccc tcacgaaatc cgggagcgcc tcaatctagc ccgaaaatgg gtaagtgagc
     2281 tgcacggaat cggcatccgg tgggtaattc cgtacacatg taacgtgaag ctgggcggaa
     2341 agtacgaagg ccggaagggc atctggaagt tctacgacca ctgggatgat tacgcctttc
     2401 tcggcatcgg tccgcggccg gaagcagacc ccatcgaatg gatggccagg gaacgtgacg
     2461 gacaacttca ttactcctat gagaagcgcc accccggctt cacgtcgtgc gggtggtaca
     2521 agtacacgcc ctgcctgaat aacccgcatt tcaataccta ccagcgagcc aacgttcgca
     2581 ccattgcgct ggccggatac gatggcgtgt tcgtggataa caacaacatc aactgctact
     2641 gccagcactg cgagaagctg ttcaaagatt ggatgagcaa gaggcacacg ctggaccagt
     2701 tgcatgagcg tttcggctgg agcacgtacg acgaggtcca cctcggctgg cgcggaaacc
     2761 ggatggactg ggtcaagtac gaccctctgt ttcgcaggtt cgtgg
//
LOCUS       DAOSOO010000314         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_36009_length_2796_cov_4.990514, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000314 DAOSOO010000000
VERSION     DAOSOO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36009_length_2796_cov_4.990514"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLT86_14300"
     CDS             <1..662
                     /locus_tag="PLT86_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37829.1"
                     /translation="RLMTEFADTGELGRVISMEWRNYGGDWLWTVDPLIESNDDPPQE
                     PIDARMPPWLPQELWGEFSVVNNGFSHGIDLMQFVAGKPTAVLSAFPAYRSGTLIVLD
                     WNGVRTLIHAASTEGSIWQEWLELRYERGWLRFATPPALHSNQPGSVEVYRGSKGITE
                     RHEPPHEWAFRREIRHFLACIRDDVPESPTPQEALDTVTIVEAVFHRAMAGQATYDIA
                     Y"
     gene            707..1696
                     /locus_tag="PLT86_14305"
     CDS             707..1696
                     /locus_tag="PLT86_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37830.1"
                     /translation="MKEIDYRTDGLDRPAIRIRAHQLVSTVCWKGGIECPLLPRDEAE
                     TILARVGSDPTASIQLTSPAERIPHYATVNPSTRSEAEKREVFNRKRDLHVLQRLGLA
                     PNDTRRARYLYTLLFDRIATPDGLCAFDTPGWEGCPLARSGAYERVREKGWSAIVYSR
                     TDAEMQAFRERSAARIASAERLFIRPHHLMCLSCWYAGGKGVGPRPNDTLYEVMVRVR
                     KNPEIPITLVEGCCMACDCCDGFHPETGRCVHDCGLIRDFKKDLDVFQKIGLMPGDTR
                     PAREVFALIYERIPSTRDICGNGTGVVTSHEWEICGGADGNPGYEATRKTGIF"
     gene            2268..>2796
                     /locus_tag="PLT86_14310"
     CDS             2268..>2796
                     /locus_tag="PLT86_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009368868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT37831.1"
                     /translation="MAHHTLKSGYSQLVERLNRFPQGAPPSEALYGILKILFGEREAE
                     LVSLLPIKPFTVDKAARAWKMPRAEAQKVLEELAGRAILIDIEQDGEMEYVLPPPMAG
                     FFEFSLMRVRGDIDQKALSELFYQYLNVEEDFVRELFTCGETQLGRVFVHEPVLSNEN
                     ALHVLDYERASEVIKT"
BASE COUNT          552 a          875 c          907 g          462 t
ORIGIN      
        1 agcgactcat gaccgagttt gccgacaccg gtgagctcgg gagagtcatc agcatggagt
       61 ggcgaaacta cggcggcgac tggctctgga ccgttgaccc gctgatcgaa tcaaatgacg
      121 atcccccgca ggagccgatc gacgcgcgaa tgcccccgtg gctcccgcag gagctttggg
      181 gagagttctc cgtggtcaac aacggtttct cgcacgggat tgatctcatg cagttcgtgg
      241 cggggaagcc gactgcggtt ctgagcgcgt tccccgcgta ccgctcggga acgctgatcg
      301 tgctggactg gaacggcgta cgaacgctga tccacgccgc ctcgacagag ggcagcatct
      361 ggcaggagtg gcttgagctg cgctacgagc gcggctggct ccgttttgcc acgccgccgg
      421 cgctccactc gaaccagccc ggttccgtgg aggtataccg agggagcaaa ggcattacgg
      481 aacgccacga gccaccgcat gagtgggcat ttcggcggga aatacgccat ttcctcgcat
      541 gcatcaggga tgatgttccc gaaagcccca cgccacagga agcgcttgac accgtgacta
      601 tcgtcgaggc ggtgttccac cgggcgatgg caggacaggc gacgtacgac atcgcgtact
      661 gaggctgcgg caaccaccaa tccacgcgct gccagcagga ctacccatga aggaaatcga
      721 ctaccgtacc gacggcctgg accgtcccgc gatacgaatc agagcccacc agctggtcag
      781 caccgtgtgt tggaaaggcg gcatcgagtg cccccttttg ccacgcgatg aggccgagac
      841 cattctggcc cgggtcgggt cagaccccac cgcgagcatc caactgacct cccccgccga
      901 acgcatcccg cattacgcca cggtcaaccc gtcgacccgc tctgaggccg aaaagcggga
      961 ggtgttcaac cgcaaacggg accttcacgt gcttcagcgt ctcgggcttg caccgaacga
     1021 cacgcggcgg gcacgctacc tgtacacact tctgtttgac cgcatcgcga ctccggacgg
     1081 actgtgcgca ttcgatacac ccggctggga gggctgcccg ctcgcgcgaa gcggggcgta
     1141 tgagcgggtg cgggagaagg gatggagcgc gatcgtctac agccgcacgg atgccgagat
     1201 gcaggcattt cgcgaacgca gcgccgcaag gatcgcctcg gccgagcggt tgttcatccg
     1261 tccacaccac ctcatgtgcc tctcatgctg gtatgccggc ggcaagggcg tcgggccgcg
     1321 gccgaatgat acgctgtacg aggtgatggt ccgggtacga aagaacccgg agatcccgat
     1381 cacgcttgtg gaaggctgct gcatggcgtg cgattgctgc gacgggttcc acccggagac
     1441 cggacgctgt gtgcacgact gcgggctgat acgtgacttc aagaaagacc tcgacgtgtt
     1501 ccagaaaatc ggactcatgc cgggcgacac gaggcccgca cgggaggtgt tcgccctgat
     1561 ctacgagcga attccctcca cacgggatat ctgcggcaac ggcacgggag tcgtcacgtc
     1621 gcacgagtgg gaaatctgcg gcggcgcgga tggcaacccc ggctatgagg cgacgcggaa
     1681 gacggggatt ttttagggag caccgcgctt ctcgggcggc gcaccggctg cttggccggt
     1741 cgtctcgata caagcaaccg gcgcttcgac atgctcccgc ccggcgggag gtcgctcagc
     1801 gaccggtttg cagctcgacg accgtgtgtg tcgtcgcccg acgaccctgt tcgtcgacgc
     1861 acgacgaacg cagaaccaag cgcgggtctc cgagtgccgt gccggacgca cagcggacgg
     1921 aacggtgtat cgaggagccc gcggcgcaaa gcctctggat gagcgctgga ttcccgcctt
     1981 cgcgtcatgc tccaccgagc acgcggaatg acggacaaag ggggagagag aaaggccccg
     2041 acggagcggg gccctccatc gggctggtgc ccaaaaacac ccgcgcggcg tttcgacagg
     2101 ctcagcgacc acacggcgtc cccaggcgat ggcatggcaa cagtctcgtc attcccgcgc
     2161 aggcgcgaat ccaggtgttt gcacagccac tcacccctga gattggaaca gcctgcccga
     2221 ggttcgataa ttgatgtcaa gacaatcacc cggaaaaggg ctctcccatg gcacaccaca
     2281 cgctgaaatc aggctattcg cagcttgtcg agcggctgaa ccgcttcccg cagggcgccc
     2341 cgccctccga ggcgttgtac ggcatcctga aaatcctgtt cggcgagcgc gaggcagagc
     2401 tggtgtccct gctgccgatc aagccgttca ccgtggacaa agccgcccgg gcgtggaaga
     2461 tgccgcgcgc agaggcgcag aaggtgctcg aggaactggc cgggcgcgcg atcctgattg
     2521 acatcgagca ggacggtgag atggagtacg tcttgccgcc gccgatggcg ggcttctttg
     2581 agttctccct catgcgcgtg cgcggcgata tcgaccagaa ggccctcagc gagctgttct
     2641 accagtacct gaacgttgaa gaggatttcg tgcgggaatt gttcacctgc ggcgaaacgc
     2701 agcttgggcg agtcttcgtg cacgagccgg tgctgtcaaa cgagaacgcg ctccacgtgc
     2761 tggattacga gcgtgcgtca gaggtgatca agaccg
//
LOCUS       DAOSOO010000315         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_36877_length_2747_cov_6.742571, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000315 DAOSOO010000000
VERSION     DAOSOO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36877_length_2747_cov_6.742571"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLT86_14315"
     CDS             complement(<1..503)
                     /locus_tag="PLT86_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT37832.1"
                     /translation="MASGNVNSVRIIGDRLEATLKKPFGERKLVYLGSLQLTPSLIDS
                     WAQKGVTIEFHAATNVWGIIVGTLPYVTILVVVYFLFRNVSGGQKGVFSFGESRAREI
                     TADMPGVTFAEVAGVEEAKQELQEIVEFLRDPQKFQRLGGRIPRGVLMVGPPGTGKTY
                     LARAIAG"
     gene            complement(950..1513)
                     /gene="hpt"
                     /locus_tag="PLT86_14320"
     CDS             complement(950..1513)
                     /gene="hpt"
                     /locus_tag="PLT86_14320"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT37833.1"
                     /translation="MSSRDSRLPEVLIPAEDIARRVTELGRKLSADYATVDVGNAGAE
                     ELVVLGVLKGAFMFLADLVRAVSIPIVVDFVRAASYGSGRVSSGDVRIEPIAWPYGEG
                     RDVLVVEDVLDTGKTLDAVVRALEGFRPRSVKTLALLRKPEAKWPVDYVGFEIEDRFV
                     VGYGLDDAGKWRQLPYIGVPRDGGERR"
     gene            complement(1506..>2747)
                     /gene="tilS"
                     /locus_tag="PLT86_14325"
     CDS             complement(1506..>2747)
                     /gene="tilS"
                     /locus_tag="PLT86_14325"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT37834.1"
                     /translation="ALAAEWHLSLRIAHIHHGLRGREADGDARWVAEFAAALDVPCDV
                     VHVDVPALKEKERGSIEDIARTARYRELKRVQSAYGMEVLATAHHLDDQAETVLHHLL
                     RGSGITGLGGMPWKTDEGHIRPLLEISRKEIRDWLRRWDVPWREDSSNASLTLTRNRI
                     RHELLPLLQKEWNPEVSRALYRLADVARAEDEAFEKWAEKALDDALVSKTRDLVLLDI
                     PCLKRYPLGLRRRVYRRAVLGLSSIEAPLSYAETAALDSLHERRTQVSFRRRFRAWST
                     RSHVAFESFDAWRGPTELLPGTRTEVPSWGAFLVTTAAGCFLRPGTDLQCQASFSSDE
                     LAMPLHVRRWRAGDRVRWGASEKLVADLLRGKKRNADPRTAVVVCDSRRVVWVPDLGD
                     IRVTAKKDTNYVTIIRESWRV"
BASE COUNT          563 a          867 c          763 g          554 t
ORIGIN      
        1 tcccctgcga tggcgcgggc aaggtaggtc ttgcccgtgc cgggtgggcc gaccatgaga
       61 acaccgcgag ggatccgccc acccaggcgc tggaacttct ggggatcgcg cagaaactcc
      121 actatctcct gcaactcctg cttcgcttcc tcgacacctg cgacttccgc gaaggttact
      181 ccgggcatgt ccgcggttat ctcgcgcgct cgactctctc cgaacgagaa aacgcctttc
      241 tgtcccccgg acacgttgcg aaacaggaaa tacacaacga cgagaatcgt cacgtacggc
      301 agggtcccga caatgattcc ccatacgttt gtggcggcgt ggaattcgat cgtgacgccc
      361 ttttgggccc aggaatcgat cagagaaggt gtgagctgca gagaacccag gtacaccaac
      421 ttacgttcgc cgaaaggctt tttcagcgtc gcttcaaggc ggtcgccgat tattctgacg
      481 gagttcacgt tgcccgacgc catgaattcc gtggtgaatt ccgtgtatga gatcgtgttg
      541 cgcgcacggg aacccgcaat ggttctccag aaaaccaccg ccagaagaag aagcaggatt
      601 acgatggtga cggttcgacc ggcagtccag ggttggcgga aacgccgccg ttttcgtggg
      661 gaaacggtgt ttttgaatcc gcgcgctcgc ggagtttggt tttccagctt gcgctgctca
      721 aggaattccg catccccact caccgcagac gggcgcgcgg actgaggagc atgcgccgtc
      781 ctgtcatcgc gggcggcacg ctggtgtgag ccgtgaaccg gggcatttct cggttgctct
      841 cgagatatgt tcccccctct tctcggtcgt ctgtttctcg gtgatttatc gttggtaccg
      901 gattgctcag cgttcatcaa cactctcttt tccgatttgg cattacctgt cagcgccgtt
      961 caccgccatc acggggaacc cctatgtagg gcaactggcg ccattttccc gcgtcgtcca
     1021 agccataacc gacgacgaac cgatcctcga tttcaaaacc gacgtagtcg acgggccatt
     1081 tcgcctccgg ttttcgcaac aacgcgaggg ttttcaccga ccgtggacgg aaaccttcca
     1141 gtgccctgac taccgcgtcc agggtttttc ccgtatcgag aacgtcttcc accacaagca
     1201 cgtcgcgtcc ctcgccatac ggccatgcga tgggctcgat ccgcacatcc cccgacgaaa
     1261 cgcggcctga accgtagctc gccgcgcgca caaagtccac aacgatgggt atggacacgg
     1321 cccgcaccag atcggcgagg aacatgaacg cacctttgag aacgccgagg acaacaagct
     1381 cctcagcgcc ggcatttccc acatcgaccg tggcgtagtc agcagacagc ttcctcccga
     1441 gttcagtgac gcggcgcgcg atgtcttccg cgggaatcaa aacctctggc aatcgagagt
     1501 ctcgactaga cacgccagga ttcccggatt atggtcacgt aattcgtgtc cttcttcgct
     1561 gttacgcgaa tgtcgccgag gtcgggcacc cagacaactc gccgactgtc gcacacaaca
     1621 accgcggttc tcgggtctgc attccgtttt tttccgcgga gcaggtctgc gacaagcttc
     1681 tctgacgcac cccatcgcac gcgatctccc gcacgccaac ggcggacatg aagaggcatc
     1741 gcgagttcgt cagaactgaa cgatgcctga cactgcaaat cggtgccggg gcgaaggaaa
     1801 cagcctgctg ccgtggttac caaaaaagcg ccccacgagg gaacttcggt gcgtgtcccc
     1861 ggaagaagct ccgtcggtcc gcgccaggcg tcgaacgatt cgaaggcaac atgactccgc
     1921 gtgctccacg cccggaagcg gcggcggaac gacacctgtg tgcgccgttc gtgcaggctg
     1981 tcgagcgccg ctgtctcggc ataactgaga ggcgcttcaa tcgacgaaag cccaagcacc
     2041 gcgcgacggt acactcttcg ccttaatcct aaagggtacc tcttcagaca agggatatca
     2101 aggagaacaa ggtcccgggt ttttgacaca agtgcatcat caagcgcctt ttcggcccac
     2161 ttttcaaacg cttcatcttc cgcccgggca acatccgcga gccggtagag cgcccgcgac
     2221 acttcaggat tccactcctt ttgcagcaac gggaggagct cgtggcgaat acgattgcgc
     2281 gtcaacgtca acgaggcgtt ggacgaatcc tcccgccacg gcacatccca acggcgcaac
     2341 cagtcacgta tctccttgcg cgatatttcg aggagcggac ggatgtggcc ttcatccgtc
     2401 ttccatggca tgccgccgag tccggtaatt ccagagccac gaagaagatg gtggaggacg
     2461 gtttccgcct gatcgtcgag atgatgagct gttgccagaa cctccatccc gtatgctgat
     2521 tgcacgcgtt tcagttcacg ataacgggcc gttctggcaa tgtcttcaat cgagccgcgt
     2581 tccttttctt tcaacgccgg cacgtcgacg tggacgacgt cgcacggtac atccagcgcc
     2641 gccgcgaact cggccaccca gcgtgcatcc ccgtcggctt ctctgccacg aaggccatgg
     2701 tggatgtggg caatccggag agaaagatgc cactctgccg caagggc
//
LOCUS       DAOSOO010000316         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37112_length_2734_cov_4.567003, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000316 DAOSOO010000000
VERSION     DAOSOO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37112_length_2734_cov_4.567003"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..274)
                     /locus_tag="PLT86_14330"
     CDS             complement(92..274)
                     /locus_tag="PLT86_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37835.1"
                     /translation="MADIRWVLANIVRVVVIVLTAGLYYSGRFLMWCADSLCDDSRAF
                     ICWIEGTDEEDEDNDG"
     gene            complement(498..722)
                     /locus_tag="PLT86_14335"
     CDS             complement(498..722)
                     /locus_tag="PLT86_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37836.1"
                     /translation="MKLTKSTIKRLKAKGFDGLYFPDGDGDAECGCKWNECSDGCPLS
                     GDRDMCLPGVKMSGGIGPRQVQDAPPITTI"
     gene            complement(781..1002)
                     /locus_tag="PLT86_14340"
     CDS             complement(781..1002)
                     /locus_tag="PLT86_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37837.1"
                     /translation="MTIEVHWPRATCPECEEVFDGVSIADDEAFELHNEFECPRCGTI
                     IEREITLAGETIGHPGRESGGNTVPVPMV"
     gene            complement(1014..2207)
                     /locus_tag="PLT86_14345"
     CDS             complement(1014..2207)
                     /locus_tag="PLT86_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37838.1"
                     /translation="MAQPWFRLYVKFATDPKMQTMSEQDQRRWIMILCLRGSDALEEI
                     LQFGGETELARNLNISPEELAKTKSALLARGLISESWDILTWDDKQYTSDSSKERVQR
                     FRATQRSKIVTLQDRYRNVTVTNRNGVTCNDPVTAPDTETETEISTPPRARIRAREND
                     TEDSILLQEEEPETPYESADNLAYVERQIIAPLNTALGNLFKDEEIRDFVRTYWRRVL
                     AGECNRIRQRRLNHLTIFGQMQAWQKSGATFDDLRRHMQKHLDRISAHDYPDHPEKYV
                     TALIGYELKNGKGPAKTSGKRFTPTIPGSIPEENHSYPEPPADLLQEWQAGLEKLRIR
                     FAERGGSLTPEQRLAALAGLTPISRSGDGVLRIGARDRNTANFAREYYGDDLAAVFGV
                     VTIEP"
     gene            complement(2301..2486)
                     /locus_tag="PLT86_14350"
     CDS             complement(2301..2486)
                     /locus_tag="PLT86_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37839.1"
                     /translation="MGKRSPKKIATGGAIYISERRALEIMAARERRNLTGMLGVLIRE
                     GAEKRGVPVQDAPQENA"
BASE COUNT          534 a          803 c          722 g          675 t
ORIGIN      
        1 cgacagtggc acccgctgac acgggatacc gtcgtcaata tgcgcgtgga caagttcgtt
       61 ctcgacgcga gcacctttgt tgcgttgcat cttacccatc gttgtcctca tcctcctcgt
      121 cggtgccctc tatccagcaa atgaacgccc gcgaatcgtc acagagcgag tcggcgcacc
      181 acataaggaa ccgccctgag taatacagcc ctgccgtcag cacgatcacg actacgcgca
      241 cgatgtttgc cagaacccaa cgaatgtcag ccaagccgac ctcccgccgc cctgagcaag
      301 ctgtatccca gccagtaggc ggcaatgacg agtgcgcaaa taatagtggt catcgttgtt
      361 cctttcgttt ttcaatcatc gcccgcaccg ccggtgtcgc cagtttgacg accttgacag
      421 cacaagtcaa agccagccga accaacggcg tccatgagcc tgtcggcggg ccggtgtttg
      481 gccgcaatgg cgacgggcta gatggtggtg atgggcggag catcttgcac ctgccttggg
      541 ccaattccac ctgacatctt cactcccggt agacacatat cacgatcacc agaaagcggg
      601 cacccgtcgg aacactcatt ccacttgcat ccgcactctg cgtcgccgtc accatccggg
      661 aaatacaacc cgtcgaaccc cttcgccttg agccgtttga tcgttgattt cgtcagtttc
      721 attgtcaatc actccccaaa gggcagtcgg ggccggggcg tggcccaccg ataatgccct
      781 ttacaccatc ggaaccggca cggtatttcc gcccgattca cgccccggat gccctatcgt
      841 ttcacctgcc agcgtgattt cccgctcgat aatcgttccg caacgcgggc actcgaactc
      901 gttatgaagc tcaaatgcct catcgtccgc gattgacaca ccgtcgaaca cttcttcgca
      961 ttctggacag gtcgcacgtg gccagtgaac ttcgattgtc atttgggcct ccttcatggt
     1021 tcaattgtga ctacaccaaa cacggcggca aggtcgtcac cataatactc gcgcgcgaaa
     1081 ttggcggtat tgcgatcgcg cgcgcctatt cgcaaaacgc catcgccact cctggaaatc
     1141 ggagtcaatc cagccagggc cgcgagccgc tgttccgggg tgagcgatcc gccacgttcc
     1201 gcgaaccgta tccgtagttt ctccaggcct gcctgccatt cctggagtaa gtccgctggc
     1261 ggctcaggat agctgtgatt ctcctctgga atggatcctg gtatggttgg cgtgaatcgt
     1321 ttgccggatg tcttcgcggg tcccttgccg tttttcagtt cgtatccgat caacgcggtg
     1381 acgtatttct ctgggtggtc cgggtagtcg tgtgcggaga tccggtcaag atgcttctgc
     1441 atgtgccggc gtaggtcgtc gaacgtcgcg ccactttttt gccatgcctg catttgcccg
     1501 aatatcgtca gatgattcaa acggcgttgc cgtattctgt tgcattcacc agcgagcacg
     1561 cgtctccagt aagttctgac gaagtccctt atctcttcgt ctttgaacag gtttcccaga
     1621 gctgtattca atggagcgat aatctggcgc tccacgtagg ccaggttgtc agctgattcg
     1681 tacggggttt ctggttcttc ctcttggaga agaatgctgt cttctgtgtc gttttctcgc
     1741 gcgcgtatgc gcgcgcgcgg tggtgtactt atttctgttt ctgtttctgt atctggggcc
     1801 gttacagggt cgttacatgt aacgccgtta cggtttgtta ctgtaacgtt acggtaacgg
     1861 tcttgtaacg ttacaatctt gctgcgttgc gttgcccgaa aacgctggac gcgttctttg
     1921 gacgaatcgg acgtgtattg cttatcgtcc catgtgagta tgtcccatga ttcggagatc
     1981 aacccgcgtg cgaggagtgc ggactttgtt ttggcgagtt cctcggggga gatgttcaga
     2041 ttgcgggcga gctctgtttc gcctccgaat tggagtattt cctccaatgc gtcagacccg
     2101 cgaaggcaca gaatcattat ccagcgccgc tgatcctgct cgctcattgt ctgcatcttg
     2161 ggatcggtgg cgaacttcac gtataggcga aaccacggtt gcgccatgtc cctcctttca
     2221 tacgcgcacg gtatctgcgc gtaaaacgta ttcaattttg aagcccctgc caccgataga
     2281 gcgacagggg cttctttgtt ttacgcattc tcctggggcg catcctggac gggcacaccg
     2341 cgcttctcgg ccccttcgcg gataagcacg ccaagcatgc cggtaaggtt ccggcgctcg
     2401 cgcgccgcca taatttccaa agcgcggcgc tcagaaatgt agatcgcccc gcccgtggcg
     2461 atctttttcg gacttcgttt tcccatacgg ctcctttctg gccaccattc gcggccttat
     2521 gaaaagtata cacaatttcc ggcgaaattg gggccgatga aaaaaaatta ttgacggtgt
     2581 aaaaaatctc ttgacactca caaccgagct gcgtatactt attgatagcg tcagggaaca
     2641 gccgcaccgg cgtacccatg acacggcgaa acggggagcg atccccgtca gggtttcggt
     2701 gtcacactct agcggaggtg gtgaacgtgg caac
//
LOCUS       DAOSOO010000317         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37197_length_2729_cov_5.140239, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000317 DAOSOO010000000
VERSION     DAOSOO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37197_length_2729_cov_5.140239"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..615)
                     /gene="recR"
                     /locus_tag="PLT86_14355"
     CDS             complement(25..615)
                     /gene="recR"
                     /locus_tag="PLT86_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT37840.1"
                     /translation="MDAVLDNAVRQFMRLPGVGRRTAQRYVYHLLRQGGEQTLALASA
                     VEALHTKIRLCSVCCSLGESDPCGICADSRRDASLICVVGETSEIAVIEGTGRYRGRY
                     HVLGGLLNPTAGIGPDKLTFPALLRRITSEVAEVIVALDASVEGDTTALYISRLLKPT
                     GVRVTTLARGLPAGGSLDHADKVTIGLSLDGRVQLT"
     gene            complement(620..973)
                     /locus_tag="PLT86_14360"
     CDS             complement(620..973)
                     /locus_tag="PLT86_14360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT37841.1"
                     /translation="MFGNLGNLAAFVKSAQEMQKRVADVQEVLKAAKVEGSAGCDLVR
                     VTANGKGDIERISVDPSLLKPEEKEALEELCSAAVNQAVARARELAQAEMARLMGGLD
                     LPPGLLDPSLLNGRP"
     gene            complement(1032..>2729)
                     /gene="dnaX"
                     /locus_tag="PLT86_14365"
     CDS             complement(1032..>2729)
                     /gene="dnaX"
                     /locus_tag="PLT86_14365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT37842.1"
                     /translation="MADERFRVLARKWRPATFSEVVGQEHVCRSLTNAIRLGRIHHAF
                     LFCGPRGVGKTTVARILAKALNCVDGPTATPCLSCYSCKSIADGSSLDVMEIDGASNN
                     NVDQVRDLRETLGYATAQSRYRVIIIDEVHMLSKAAFNALLKSLEEPPPHVVFIFATT
                     EATKVIPTVQSRCQRYDFRRLSTRDIRDQLTMICREEKVDVEPAGIDLIAQYADGAMR
                     DGQSLLDQVIAATEGHVTASEVADLIGAVERTVFFDLVDAIARRDVSATISAVADTVF
                     RGRSLAQFLSGLMSHLRCLLACAANARNALSDFEPEELERLSAQARKFSDRDILRMLS
                     LAADAESLLEKSAVPQVRVELALLKMVYMEKSVELSDLVGRLDTLLTGPPAAPPHPVA
                     RGPAPPETAETRTATISTPSAVPAKRIAADLTSPDHPPPVRRESEEDQPGTPGLGLLT
                     ARWPDIVDSVKSASSAVPWFHQVRPTGIDGDTVILEVDGAFFAERVSRMDESLLSAIR
                     AHFPAVRRISVRAVDKSGQNSDVSETVRRAGSLIQGVVEEEPVIGKLIESLGLELIE"
BASE COUNT          550 a          829 c          798 g          552 t
ORIGIN      
        1 aaagcgagct attgctagct cgtcctacgt gagttgaacc cgtccatcga gcgacaaccc
       61 gatggtaacc ttgtccgcat ggtcaaggga gccgccggcg ggcaatccgc gcgcgagcgt
      121 cgtgacgcgc acgccggtgg gtttgagaag ccgcgaaatg taaagtgcgg tggtgtcacc
      181 ttcaacgctg gcgtccagtg ccacgatgac ttcggccacc tccgaagtga tcctccggag
      241 cagggcgggg aacgtcagct tatccggtcc aattcctgcc gtcgggttga gcaaaccgcc
      301 gagaacgtgg taacggccgc ggtatctgcc agtcccttct atcaccgcga tttcgctggt
      361 ttctcccaca acacagatca gggaagcgtc ccgccgggaa tccgcgcaga tgccgcaagg
      421 atcgctttcc ccgagcgaac agcacacgct gcaaagcctt atttttgtgt gcagtgcctc
      481 tacggcagac gcgagcgcca gtgtctgttc gccgccctgc cgaagaagat ggtacacgta
      541 acgctgtgcc gttcgcctcc ccactccagg caaacgcatg aattgccgca ccgcattgtc
      601 aaggactgca tccacctggt tacggcctcc cgttcagtaa cgacgggtcc agcaaccccg
      661 gcggaaggtc caggccgccc atcagtctgg ccatttcagc ctgcgccagt tctcttgcgc
      721 gggcaactgc ctgattcacc gcggctgaac acaactcctc gagggcttct ttttcctccg
      781 gcttcaggag agaggggtca acggaaatgc gttcgatgtc tcccttgccg tttgcggtga
      841 ccctgaccag atcgcagccg gcggagccct caactttcgc agccttcagg acctcctgaa
      901 cgtccgccac gcgcttctgc atttcctgag cgcttttgac gaacgcggca aggttgccaa
      961 ggtttccgaa catcgcgccg tgtgctccgg tttgggtatt tgtcgattct taggaatcga
     1021 ggatcaggct ttcattcgat cagttcaaga cccagggatt cgatcaactt gccgatcacc
     1081 ggttcctctt cgacaactcc ctgaatcagt gaaccggccc ggcgtacggt ttcagaaacg
     1141 tcgctgttct ggccgctctt gtcaacagcg cggaccgata tgcggcgtac ggccggaaaa
     1201 tgagcgcgaa tcgcactgag caggctctcg tccatgcgcg aaacgcgttc ggcgaaaaat
     1261 gccccgtcga cttccagaat caccgtgtcg ccatcgattc cggtcggacg cacctgatga
     1321 aaccaaggta ctgcggagga ggccgatttc acggaatcca cgatgtccgg ccagcgggca
     1381 gtcaacagac caagacccgg cgttccaggc tggtcttcct cggattcccg gcgaaccggc
     1441 ggtgggtggt caggagatgt gaggtcggcg gctattcttt ttgcgggaac cgccgaaggc
     1501 gtggagatgg tcgctgttcg cgtttctgcc gtctcaggcg gagcaggtcc ccgtgcgaca
     1561 ggatgaggcg gcgccgcagg cggacccgtc agaagcgtgt ccaatcgccc tacaaggtcc
     1621 gagagctcaa cggatttctc catgtaaacc attttgagca acgcaagttc tacacgaacc
     1681 tgcggcacgg cggacttttc cagaagcgat tccgcatccg ccgcaagaga cagcatcctc
     1741 aaaatgtccc ggtcagagaa tttgcgcgcc tgggcggaaa gccgctcaag ctcttccggc
     1801 tcgaaatcgc tcaaggcatt ccgggcattg gccgcacagg ccagcagaca cctcaaatgg
     1861 gacatcaacc ccgaaagaaa ctgcgccagg cttcttccgc ggaacaccgt gtctgcaact
     1921 gcagaaatgg tggccgaaac gtctcttctg gcgatcgcgt ccacgaggtc aaaaaacacg
     1981 gtgcgttcga ccgcaccgat gaggtcggca acctccgagg cggtaacgtg accttcggtc
     2041 gcggcaatca cctggtccag cagactctgc ccatctcgca tggcgccatc agcgtactgc
     2101 gcgatcaggt cgatccctgc gggttcgacg tctacctttt cttcgcggca gatcatcgtc
     2161 agttggtcgc gaatatctcg agtgctaagg cgccgaaagt cgtaacgttg gcaccgggat
     2221 tggacagtcg ggatgacttt cgttgcttcc gtggtggcaa aaataaagac tacgtgcggg
     2281 ggtggttctt cgagcgattt caacagggcg ttgaacgcag ccttcgagag catatgcact
     2341 tcgtcgatta tgatgacgcg gtaccggctc tgggcagttg cgtatccgag cgtctcgcgc
     2401 agatcccgaa cctgatcgac gttgttgttt gaagcgccgt cgatttccat cacgtcgagg
     2461 ctgcttccgt ctgcgatgga tttgcacgaa tagcaggaaa ggcagggcgt cgcagtggga
     2521 ccatcgacgc agttcagcgc tttggccagg atgcgcgcaa cggtggtttt tccgactccc
     2581 cgcggaccgc agaagaggaa cgcatggtgg atgcggccga ggcgaatcgc gttcgtcagg
     2641 ctgcggcaca cgtgctcttg accgacaacc tcgctgaacg tagccggacg ccatttgcgc
     2701 gccagaacgc gaaagcgctc atctgccat
//
LOCUS       DAOSOO010000318         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37266_length_2725_cov_4.603745, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000318 DAOSOO010000000
VERSION     DAOSOO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37266_length_2725_cov_4.603745"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PLT86_14370"
     CDS             complement(<1..1577)
                     /locus_tag="PLT86_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT37843.1"
                     /translation="MSSLTRLWTFETAGFVGCPAVLPTQDGTLLVCGTNSGHIVAIDS
                     DRNEHWRTPVGSKLGTWVSVSSANDPDRCVLAVTEAGKAIRLSDDGTLVWASDVEAAT
                     SPFNSVGIAFGGQVSRVIVTDAKGRVTGLALDSGEHAWQFHTNVRAVGPAAIGDIDRD
                     GEDEIVFCAGDSFIYCIDLNGRLLWRVKTDAPAEFSAPVLVDFGQGPRVLAGSMDDVL
                     RCITPEGEVAWTCRGAGAGSIEIGIAAGDINADGVPEIVFVHAGRAIQAVASDGTLIW
                     ANTDSGDGDQPFAPSLVDVDRDGRLESLLVQRAGTTLRVFDSEGVQIENYRLEGTMIG
                     APVAADLDGDGMLEIFTVNATTGALSCFKSLGGKTEWPTSRGAFDGRANNLRTGRPLA
                     RKPETPRDLPAVVGPKGRLRLGANALDYDLPSGTYASVSLRGSDGVTHFGVTDSQRRV
                     EFEILTCGEYTVQATIYRDADRAPIGAASSQFSVTLFQDEQAEIERLLKHLSGIAGGY
                     GITNDLVRGTRTRKLTW"
     gene            complement(1601..2407)
                     /gene="dapB"
                     /locus_tag="PLT86_14375"
     CDS             complement(1601..2407)
                     /gene="dapB"
                     /locus_tag="PLT86_14375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT37844.1"
                     /translation="MVRIAVCGSAGRMGRRIVALAHEDPELSITGAVEKPGDPAIGCD
                     AGEIAGVGRLSVPVVNNLADAIKGADVAIAFINNPEATVEQAAICRAAAKPLVLGTTG
                     LTSEQEQAFKDAAQGIAVVKTSNFSVGVTVLLRLVEEAAHLLGPRFNCEIVEAHHTLK
                     RDAPSGTAISLAKAVAKTWGWDFEKSVRHGRTGITGERPVDEIGMHSLRAGDIVGQHD
                     VLFGGVGESIELRHRAQSRDTFAMGALRAAKWVVGKPEGLYDMFDVLALR"
BASE COUNT          535 a          845 c          782 g          563 t
ORIGIN      
        1 tgccacgtga gcttccgggt gcgcgttccc cgcaccaggt cgttcgtgat gccgtacccg
       61 ccggcaatgc ctgagaggtg cttcagcagg cgctcaattt cagcctgttc atcctggaaa
      121 agggttacgg agaactggct cgaagcggcc ccgatcgggg cacgatcggc atcgcgatat
      181 atcgttgcct gcaccgtgta ttcgccgcag gtaaggattt cgaactcaac cctgcgttgc
      241 gaatccgtga cgccgaagtg ggtgacccca tcagaacccc gaagtgagac gctggcatac
      301 gttccggacg ggagatcgta atcaagggcg tttgccccga gccgcaatcg acccttcggt
      361 ccaaccacag cgggaagatc gcggggcgtc tcaggttttc tggccagcgg tctccccgtg
      421 cggagattgt tggcgcgccc atcgaatgcg ccgcggcttg tcggccactc cgttttgcct
      481 ccgaggcttt tgaaacaact caaggccccg gtggtggcgt ttacggtgaa gatttcgagc
      541 atcccgtcgc cgtccaggtc ggcggcgacg ggcgccccga tcatcgttcc ctcgaggcgg
      601 taattctcga tctgcacccc ctcggaatca aacacgcgaa gagtcgttcc ggcgcgttga
      661 acgagcagcg attccaacct gccgtcgcgg tcgacatcta ccagggatgg tgcaaatggt
      721 tggtcgccat ctcctgaatc cgtgtttgcc cagatgagcg tgccatccga cgcaaccgcc
      781 tggatcgccc ggccggcgtg gacaaatacg atctccggaa caccgtcggc gttgatgtcg
      841 ccggctgcga tgccgatttc gatgcttccg gcccctgcgc cacgacaagt ccacgcgacc
      901 tcaccctcag gggtgatgca gcgaaggacg tcgtccatgg accctgccag tacgcgtggg
      961 ccttgcccga aatcaaccag taccggtgca gagaattcag ccggtgcatc agtcttgacg
     1021 cgccagagga gccttccatt cagatcgatg caatagatga acgagtctcc cgcgcaaaag
     1081 acgatttcat cctcgccgtc tcgatcgata tcacctattg ccgcgggtcc cacggctcgc
     1141 acgttcgtgt ggaactgcca cgcatgctcc ccggaatcaa gcgcaagacc ggtgacacgt
     1201 cccttcgcgt cggtcacgat gacgcgggag acctgcccgc cgaatgctat tccgacgctg
     1261 ttgaaggggc tggttgcggc ttcgacgtcg gacgcccaga cgagcgtacc gtcgtctgaa
     1321 agacggatcg ccttccctgc ttccgtgacg gcgagcacgc atctgtcggg atcattcgca
     1381 gacgatactg aaacccatgt ccccaacttc gaacctactg gcgttctcca gtgctcgttg
     1441 cgatctgaat cgatagcgac aatgtgcccc gaattggttc cacagaccag gagtgtgccg
     1501 tcctgcgtgg gaaggacggc cggacaacct acaaatccgg cagtttcgaa cgtccacaaa
     1561 cgggtgagcg aactcattgc gttccctccg gcctgaggtt tcaccgtaaa gccagaacat
     1621 caaacatgtc gtacaaccct tcgggtttgc ccacaaccca ttttgcggca cggagtgccc
     1681 ccatcgcaaa cgtatcgcgg ctctgggcac ggtggcgcag ttctatcgat tcgccgacgc
     1741 ccccgaacaa gacgtcgtgc tggccgacga tatcccccgc ccgcaacgag tgcattccaa
     1801 tctcatcaac agggcgttcc ccggtgatgc cggttcttcc gtggcggacg gatttttcaa
     1861 aatcccagcc ccatgttttc gctactgctt tcgccagcga gatggcggtc ccgctcggtg
     1921 cgtctcgctt caatgtgtgg tgggcttcca ctatctcgca attgaaccgt ggtccgagta
     1981 gatgcgccgc ttcctcaacc aggcgcagaa gcacggttac gccaacactg aaattgcttg
     2041 tcttcacaac cgcaatccct tgcgctgcgt ctttgaacgc ctgttcctgc tcagacgtca
     2101 ggcccgtagt tcccaggaca agcggctttg cggcggctct gcagatggcg gcctgctcca
     2161 ccgtggcttc cgggttattg atgaaggcaa tggcgacatc tgcgcctttg atggcatctg
     2221 cgaggttatt gacgacggga accgagagcc tcccgacgcc cgcgatttca cctgcatcgc
     2281 aaccgattgc cgggtcaccg ggtttctcca cggctcccgt gatgctgagt tcggggtctt
     2341 cgtgcgccag ggcaacgatg cgccgaccca ttcgtcctgc cgatccgcaa actgcgattc
     2401 gtaccatgaa cctcacgctc ctgttatcgt gttgcgccag gccgcgggtc cgaagcgaac
     2461 ccgattcgtg cattcctagt atagagccag accgacaact ggaggaagtg ctctgtttgc
     2521 tggggatccg ccgattggtg gcggatagtg catcaatggc agcaaaatgc aattcttgcg
     2581 cggttgcgtg gcgaaaaaca gcaccaagga gggtacacat agcgtaaatg tgggcatgta
     2641 gagttgaagt gcaacaatgg cacagtcatt gcatgctaga attccgggtt cttcgaggtg
     2701 caagggacaa gcggagcacg gaacc
//
LOCUS       DAOSOO010000319         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37458_length_2715_cov_2.615789, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000319 DAOSOO010000000
VERSION     DAOSOO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37458_length_2715_cov_2.615789"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLT86_14380"
     CDS             complement(<1..1059)
                     /locus_tag="PLT86_14380"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT37845.1"
                     /translation="MRNIQDFPCHGSLKATEAYTHASAESTQASQVPSAIFIEKGLET
                     VKKAILRLLRQAKHDSAVAIVCFTVAFSSSTLWADNQIIPGNLGTSDPAPMIEGNTLY
                     LYTTADAIGNGDLSIYDIQVHSTTDLVKWKDEGIALTEKNVPWAAQKGNLWAPHVIKL
                     GGKYHLYFPANDGRVFRMGHAVSDKPTGPFIADATFMAGCGVDAIDPFAFYDSTTNKA
                     YLAWNQVGSTPNQAYIAELNSTFSNVTGTVTNIRAGLGGNSQRYIEGIWIIKQNDLYY
                     NIVADWTGSVESITYSTSTNLRGPYTYAGEILNMNANAATIHAGAVFFRNRWLIFYHT
                     GGNEFGGTINTGTKRVTGI"
     gene            complement(1214..>2715)
                     /locus_tag="PLT86_14385"
     CDS             complement(1214..>2715)
                     /locus_tag="PLT86_14385"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOT37846.1"
                     /translation="VAFSNGGKAYWYRHYWASFMHAHSIATGNTRTASYNVDGDWYTP
                     PFKGSTKEGEDAIWVTGWDKLFKLTYNPSSNSISRTDLPKDFKAEAGDVTYGVGVAKS
                     DPNRLYVATRNGRFFHSRDGGQTWTETTYSGPKPTPGRTAWNMSSGLYIEVSDSVPDL
                     VFWGGGTGSVACLVSRDGGKTFVSNVTGLPSGGEVRNVSVAPDGKMAFSSNFHVWIAL
                     DNRWYDMRSASMPSAALPAANALNYLPLQRKVRYFTWGAGVVDLDIAELNTADNPKLD
                     LGAGSCIQIKSASSGRNLAATAGGVVVQASSNGAANQGWRIVPEDNFWRIENSATGGV
                     LQVASASHKDAAAVQAAPWSGGDHQQWNVVRDGASYAIVARHSVKALQIAGTSTADSA
                     KAEQGTYLAASNQKWTLVPGSNCGTTPTLRGAPVQRIRLGRAANGAFRIEGLEGDPAH
                     VLAWDQNGRIVWRGEVGERTDLPRLGAGLYRVRVWSNHLDVTLTGTQLR"
BASE COUNT          482 a          813 c          807 g          613 t
ORIGIN      
        1 gataccggtg acccgtttgg ttcccgtgtt gattgttcct ccgaactcgt tccctccggt
       61 gtggtaaaaa atcagccatc tgtttctgaa gaacaccgcc ccggcatgta ttgtggcagc
      121 gttggcgttc atgttgagga tctctcccgc atatgtataa gggccacgca ggttggttga
      181 cgtcgagtac gtgattgatt cgaccgagcc ggtccagtct gcaacgatgt tgtaataaag
      241 atcgttctgc ttgatgatcc agatcccttc aatgtatcgt tgcgagttgc ccccaagccc
      301 ggctcggata tttgttaccg ttcccgtgac gttggaaaac gtggaattca actctgcgat
      361 atacgcttga ttcggggtgc tgccaacctg attccaggca aggtacgcct tgttggttgt
      421 ggaatcataa aacgcaaatg gatcgatggc atcgacccca catcccgcca tgaaggtcgc
      481 gtcagcaatg aatgggcctg tgggtttgtc ggaaacggca tgccccatcc gaaagaccct
      541 tccgtcgttc gcaggaaaat agaggtggta ttttccgccc agttttataa cgtgtggcgc
      601 ccacagattg cccttctgag ctgcccatgg gacatttttc tctgtcaggg caatcccctc
      661 atctttccat ttcactaggt ctgtcgtgga gtggacctgg atatcataaa tggacaggtc
      721 tccgtttccg atagcatcag ccgtggtgta caaataaagc gtattgcctt cgatcatcgg
      781 agcagggtcg gaggtgccga gattcccggg aatgatctga ttgtctgccc acagtgtact
      841 gctcgagaat gcaactgtaa aacagacaat agccacagcg ctatcgtgct tggcttgccg
      901 caacaacctc agaatcgctt ttttcactgt ctccaatcct ttttcgatga agattgccga
      961 ggggacttgg gatgcttgcg tcgactcggc ggatgcatgg gtgtaagcct cggtggcttt
     1021 caagctgccg tggcatggga aatcctggat gtttcgcatt tccggccgcc ttcgatcatg
     1081 tgggctgctg ggatcatgta aaccttcccg tgtctgtggc tgttgtgttg gacgtatgca
     1141 gacttcccgg tcacggtggg agcgccgctc ccggcttccc gggagcagtg ctcccaccgt
     1201 gaagcgtttt tgatcaccgc aactgggtcc cggtcaaggt gacatccagg tggttcgacc
     1261 agacccggac gcggtagagc cccgccccga gtcggggcag gtcggttcgc tccccgactt
     1321 caccacgcca cacgatgcgt ccgttttgat cccatgcaag gacatgcgcc ggatcgccct
     1381 caagcccctc gatgcggaac gctccgttgg cggcccttcc cagtcgaatc cgctggactg
     1441 gagcgcctct gagggtaggc gtcgtgccgc agttcgagcc gggaaccaac gtccacttct
     1501 ggttcgacgc ggcgaggtag gtgccttgct ccgccttggc gctgtccgcg gtcgatgttc
     1561 ctgcgatctg cagggccttg acgctgtgcc ttgccacgat ggcgtagctc gccccatcgc
     1621 gaaccacgtt ccactgctgg tggtcgccac ccgaccaggg ggccgcctgc accgctgcgg
     1681 cgtccttgtg ggaggccgat gccacctgca ggacgcctcc cgtcgcggag ttctcgatgc
     1741 gccagaaatt gtcttcaggc acgatgcgcc aaccctggtt cgcggcgccg ttcgaggatg
     1801 cctgcacgac cactcctcca gccgtcgccg cgagattcct tccgctggat gccgacttga
     1861 tctggatgca cgagcccgcg cccagatcga gcttcggatt gtccgcggtg ttcagctccg
     1921 cgatgtcgag atccacgacg cccgcgcccc aggtgaaata gcgcaccttc cgttgcaagg
     1981 gcaggtagtt cagggcgttg gcggcaggca gcgcggcgga gggcatgctc gccgagcgca
     2041 tgtcgtacca gcgattgtcc aaagcgatcc agacgtggaa gttcgagctg aacgccatct
     2101 tcccgtcggg ggcgacgctg acgttgcgga cttctcctcc cgaagggagt cccgtcacgt
     2161 tggagacgaa cgtcttgcct ccgtcgcggc tcacaaggca ggccacggag cccgtgcctc
     2221 ctccccagaa caccagatcg ggaacgctgt cggaaacctc gatatagagt ccggaggaca
     2281 tgttccaggc ggtcctcccc ggtgtcggct tcggccccga ataggtcgtc tccgtccacg
     2341 tctgcccgcc atcccgggaa tggaagaacc gcccgttcct ggtcgccacg tagaggcggt
     2401 tcgggtcgga cttcgcgacg ccgacgccgt aggtcacgtc gccagcctcg gccttgaagt
     2461 ccttgggcag gtccgttcgc gagatgctgt tggaagacgg gttgtaggtg agcttgaaga
     2521 gcttgtccca gcccgtgacc catatggcgt cttctccctc cttcgtcgac cccttgaatg
     2581 gaggcgtgta ccagtctccg tcgacgttgt aggaagcagt gcgggtgttg ccggtcgcga
     2641 tggaatgggc atgcatgaac gacgcccagt agtgccggta ccagtaggcc ttgcctccgt
     2701 tgctgaaggc cacgc
//
LOCUS       DAOSOO010000320         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37782_length_2698_cov_4.362467, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000320 DAOSOO010000000
VERSION     DAOSOO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37782_length_2698_cov_4.362467"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLT86_14390"
     CDS             <1..649
                     /locus_tag="PLT86_14390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT37847.1"
                     /translation="AYNVYGMVRGSTHPQELIIVISHKDSQSWMVNTAGAYDNGIGTV
                     ANLEIARVLQGCKPKRTLWFVFCNEEHAPWTSAVTAQRVKDEGYALVAALNVDSLGGK
                     SAEDQKAGVKTNATRYVTDEGERIADLMARLNEELGIGLRQTKHRGEFPNDDDGSFIK
                     AGMPAAVAMVGSFPYAEPNYHLASDVAENVDIENARMATQLALATLLHLDENGPL"
     gene            797..1828
                     /gene="hrcA"
                     /locus_tag="PLT86_14395"
     CDS             797..1828
                     /gene="hrcA"
                     /locus_tag="PLT86_14395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT37848.1"
                     /translation="MAMRPLTPREIAVLTAVVHAHTESAEPVGSRTIARQYLSDLSPA
                     TIRNTMMDLEDIGFLHSPHTSAGREPTARAYRYYLDEVMEPARLGRSDKSVFDELLTE
                     RQCARDADVVLSCVSRALARVSRLITVAFLPTFDNGVLRRIELVSLGESRILVALEIK
                     GGPVHTLTLEMNEPVAQGLIGETARILNERLSGLTIGQIRTSIAERLSGISGGEQHVI
                     NVFLREGSDIFDIDSRTNVHLEGRPNILAQPEFSDHTRLMQFMRLLDEKTLIQELRRR
                     GVTRRTQVSIGTENSLPELADCSLLTRGYQAGSLSGTLAIVGPMRMPYRRLVAVLEHA
                     GGVAETLMS"
     gene            1887..>2698
                     /locus_tag="PLT86_14400"
     CDS             1887..>2698
                     /locus_tag="PLT86_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37849.1"
                     /translation="MRWRFLPGILLAAPLLWPGCCGDCIVGFNRRCPEVIADVPRHTP
                     NAFFAWAYGGHPGFEGTTTRPVTILFQKKAAADSGYFQLSCSPGADANYCGVVNRFCV
                     DAVSFDTAYGLARYRTPDSRGSAANDPAYYIALPNDSVTVTSNRIQSAISSSPTVYDT
                     TLVTLYANTTADPPTLMPDRTPLVTLAWAKYPLATQAVLLTNDVQGNLLSGVVILAGT
                     TTNPTTWTYGAPAARVLRTSQPLQSGTPYTLTVWLLNNGNWSLAVDQVTFTP"
BASE COUNT          525 a          887 c          814 g          472 t
ORIGIN      
        1 cgcgtacaac gtctacggca tggtgcgcgg ctccacgcac ccgcaggaac tcatcatcgt
       61 catctcccat aaggactcgc agagctggat ggtgaacacc gccggcgcct acgataacgg
      121 catcggcacg gtggccaacc tcgagatcgc ccgggtgttg cagggctgca aaccgaagcg
      181 cacgctctgg ttcgtgttct gcaacgaaga gcatgcgcca tggacgagcg cggtgacggc
      241 gcaacgggtg aaggatgagg gatacgcgct ggttgccgcg ctcaacgtgg acagcctcgg
      301 cggaaaatcg gcagaggatc agaaggcggg ggtcaaaacc aacgccacgc ggtatgtcac
      361 cgacgagggc gaacgcatcg ccgatctgat ggcacggctg aatgaggagc ttggcatcgg
      421 tctgcggcag accaagcacc ggggcgaatt cccgaacgac gacgatggct cgttcatcaa
      481 ggccggtatg cccgcggcgg tggcgatggt ggggtcgttc ccctacgcgg agccgaacta
      541 tcacctggcg agcgacgtcg cggagaacgt ggacatcgaa aatgcgcgga tggccacgca
      601 actggccctt gccacgctgc tccatctgga cgaaaacggc cccctgtagg tcacggaagt
      661 ggttcaactt gacaagtgcc ttcgccgcgg gtatgtttag ggagggttta gcactccaag
      721 gtggagagtg ctaaacccga tgctgaaatt cggtagcccg ctccgccggt ggcgggctgc
      781 aacgaccgga gggtaaatgg cgatgcgacc acttactcca cgagaaatcg cagtgctgac
      841 tgcggttgtg cacgcgcaca ccgagtcggc ggaaccggta ggctcccgca cgatcgcgcg
      901 gcagtacctc agcgacctca gcccggcgac catccgcaac accatgatgg atctggagga
      961 catcggattc ctccattcgc cgcacacctc ggcgggcagg gaaccgacgg cccgggcata
     1021 ccggtactac ctcgacgaag tcatggaacc ggcgcgcctc ggccgttccg acaaatcggt
     1081 gttcgacgag ctcctgacag aacgccagtg cgcccgcgac gcagatgtcg tcctttcgtg
     1141 cgtctccagg gcgcttgccc gcgtatcccg tcttatcacg gtggcgtttc tccccacgtt
     1201 tgataatggc gttctgcggc gtatcgagct tgtttcgctg ggcgaaagcc gcatccttgt
     1261 ggcgctggaa atcaaaggcg gccccgttca cacgctgact ctggaaatga acgagcccgt
     1321 tgcgcaggga ctgatcggtg aaaccgcccg catcctgaac gaacggctca gcggcctgac
     1381 tatcggccag atacgcacgt ccatcgcgga acggttgagc ggcatctctg gcggcgaaca
     1441 gcacgtaatc aacgtatttc tccgcgaagg atcggacatt ttcgacatcg attcgcggac
     1501 gaacgtgcat ctggaagggc gccccaacat ccttgcgcaa ccggagttca gcgaccacac
     1561 ccgcctgatg cagttcatgc gccttctcga cgagaaaacg ctgattcagg aactgcggcg
     1621 gcgtggcgtt acgcggcgaa ctcaggtgtc catcggaacc gagaattcgc tgccggaact
     1681 tgccgattgc agcctgctga cgcgagggta tcaggcgggc tctctttcgg gaacgctggc
     1741 cattgtcggc cccatgcgca tgccgtaccg ccgccttgtc gccgttctgg agcacgccgg
     1801 aggcgtcgcc gaaacgctta tgagttgagt gcggcgggaa agggaacccc cttctgaacg
     1861 gatgcctctt cctgaaagga agattcatga ggtggcgctt cctccccggc attctgctgg
     1921 cggcccccct cctctggccc ggctgctgcg gagattgcat cgtcgggttc aaccgccggt
     1981 gcccggaagt gatcgccgat gttccccgcc acacaccgaa tgcctttttc gcctgggcgt
     2041 acggcggcca ccccgggttc gagggcacga ccacccgccc cgtcaccatc ctgttccaga
     2101 aaaaggccgc agcggattcc ggctatttcc agctttcctg ctcccccggt gctgatgcca
     2161 attactgcgg cgtggtgaac cgtttctgcg tggacgccgt ctcgttcgac accgcgtacg
     2221 gccttgcgcg gtaccgcacc ccggattccc ggggaagcgc cgcgaatgat ccggcatact
     2281 acatcgccct ccccaacgac tcggtcaccg tcacatccaa tcgcatccag tcggccatat
     2341 ccagttctcc aacggtgtac gacacgacgc tcgtaacgct ctacgcaaac accacggccg
     2401 acccgcccac cctgatgccg gacaggaccc cgctggtaac cctggcgtgg gcgaaatacc
     2461 cgcttgcgac gcaggcggtt ctcctcacaa acgacgtgca gggcaatctc ctctccggcg
     2521 tggtgatact ggcagggacc accaccaacc ccaccacgtg gacgtacggc gcccccgccg
     2581 cgcgcgtgct ccgcaccagc cagccgctgc aatccggcac accgtacacg ctcaccgtct
     2641 ggctgctgaa caacgggaac tggagcctcg ccgtggatca ggtgaccttc acgcctca
//
LOCUS       DAOSOO010000321         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37873_length_2693_cov_4.572782, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000321 DAOSOO010000000
VERSION     DAOSOO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37873_length_2693_cov_4.572782"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLT86_14405"
     CDS             complement(<1..1139)
                     /locus_tag="PLT86_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOT37850.1"
                     /translation="MTDSKGAFIFGAFCAYMTLIYCIISSVIVLSVLDIRLTQFNAVL
                     ILVFAASLSVLLLTRTKGLVSGNESSPIPMTTNQRLRTALAVAAVMLVVGWVFLVVIA
                     VRLPDFTCDGNFYHIPAIQAYVNAGKVHWIAGLPANLDGYMNGYPKGAEVLSFYIVTL
                     LQSDSSVNLANVLFLPMGVLGIATVCGLLGIDFLVSFCIGLLWVTIPVNIFQSATAYV
                     DSAFASAVVGAVAAFFSHLVLVDTNRKKTYSVVLLGISLGLALGVKGTGAVVCATVLL
                     AFTIVAVARRVRRRDAGILVESIGVVAVVLVTSLPSGLFWYCRNYAHTGSPVYPAGLT
                     LLGRIIFPGPPVSVAVGLEGQVPEVLRELNPVWRVVRTWLQIAGG"
     gene            complement(1183..2472)
                     /locus_tag="PLT86_14410"
     CDS             complement(1183..2472)
                     /locus_tag="PLT86_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37851.1"
                     /translation="MNVGTAWEDFTPSRPLHLLGQMHVRLGQHARDPLTANAVVLDDG
                     TTRVAMVSVDVCVLPKERVDALRAACASACEIDPESVIIAATHTHVAPCTANLVGDIC
                     PEFLETLEQAVARAVGRAIEDLEDASLFAGTGFIEQMGWNRRGLHRDGSAGMYLGSWA
                     EGFVGIEGPRDGSVPVIFARRADGSVKAVLTGFSTHPNTLEGESFYSADIPGEVRRVL
                     RATLGDGVGVVYFTGAAGNTAPSIMVDNPQNIQPWRGEQGLRRSGQYLGGEILKTIAE
                     QTDPMPDPVLRHGHAEIGIPIRPWDEDSDFSQFGGGMLEFFEKSKADWDRVLREESPV
                     ATPIDVVRVGDAALCFNPAELYCEFGLAIKEKSPARVTLVSELANAWVGYVPTPQAIR
                     HGGYSAQSSWVTKLAPDAGWMIVEKTRELLAEVFGER"
     gene            complement(2497..>2693)
                     /locus_tag="PLT86_14415"
     CDS             complement(2497..>2693)
                     /locus_tag="PLT86_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37852.1"
                     /translation="WTSAFARKDVSYDLSQYPVSQQCIDTCLWGVFNHRPPNGPEQIN
                     ALADAIRKVFENLDDVPVEA"
BASE COUNT          613 a          864 c          719 g          497 t
ORIGIN      
        1 tgcccgcccg caatttggag ccaagttcgg accacccgcc aaacggggtt cagttcgcgc
       61 agaacctcgg gtacctgccc ttccaacccg acggcgaccg aaacgggtgg tcccggaaat
      121 atgattcggc cgaggagagt gagcccagca gggtacactg ggctcccagt atgcgcgtag
      181 ttgcggcaat accagaacag accggacggc aaactggtaa cgagcacgac ggcaaccaca
      241 ccgatggact ccacgagaat tccggcgtcc ctgcgtctaa cacgccgtgc aacggcgact
      301 atcgtgaagg caagaagaac ggtggcgcag acaacagcac ctgtcccttt gacacccaac
      361 gccaaaccca gactgatgcc caagagaacg acactgtatg ttttttttct gtttgtgtcc
      421 acaagaacga gatgggaaaa aaatgccgca accgctccga ctacagccga ggcaaacgcg
      481 gaatccacat atgccgtcgc actttggaaa atgttgaccg gtattgtgac ccacagaaga
      541 ccaatacaaa acgaaacaag aaagtcgatt ccaagcagcc cgcatacggt tgcaatgccc
      601 aaaaccccca tcggcaaaaa caggacattg gcgagattca ccgaggaatc actctggaga
      661 agcgtgacaa tgtagaacga caatacctcg gcgcctttgg gatatccgtt catgtacccg
      721 tccaagttgg cggggagtcc ggctatccag tgtacctttc cggcgttgac gtaggcttga
      781 atcgcgggaa tatggtagaa attcccgtca cacgtgaaat cggggagacg cactgcgatg
      841 accacgagaa aaacccaccc aacaaccagc attactgccg cgacagcaag cgcggtgcgc
      901 aaccgctgat tcgtcgtcat gggaattgga gacgattcgt tcccggacac caaacccttg
      961 gttctcgtga gcagcaaaac agatagcgat gcggcaaaca caaggatcag caccgcattg
     1021 aactgcgtaa ggcggatatc caacacgctc aacacgatta ctgaggagat gatgcaataa
     1081 atcagcgtca tataagcgca gaaggcgcca aaaatgaaag cacctttgct gtctgtcatt
     1141 tccgcctccg ttccgctggg tctgctgcac gaaaatgtca ttctatcgct ccccaaacac
     1201 ctccgccagc agctcccgcg tcttttccac aatcatccat cctgcatccg gggcgagctt
     1261 cgtcacccac gacgactgcg cgctgtagcc gccatgccgg atcgcctgcg gagtcggcac
     1321 gtaccccacc caggcattcg ccagttcgct gacaagcgtt acccgggccg gcgacttttc
     1381 cttgattgca aggccgaact cgcagtagag ctccgcaggg ttgaaacaca gcgccgcatc
     1441 gcccaccctc accacgtcaa tcggcgtggc gacggggctc tcttcgcgca acacgcggtc
     1501 ccagtccgct ttgctcttct cgaagaactc cagcatcccg ccgccgaact gagagaaatc
     1561 ggagtcctcg tcccacgggc ggatcggtat gccgatttcc gcgtgcccgt ggcgcaggac
     1621 cggatccggc atcggatcag tctgttctgc gatggttttc agaatttcgc cgccgaggta
     1681 ctgacccgac cgccgcaagc cctgttcgcc ccgccacggc tggatgttct gcgggttgtc
     1741 caccatgatg ctgggcgcag tgttgcccgc cgcaccggtg aagtacacaa cgccgacacc
     1801 atcgccaagc gtagctctga gcactcgccg cacctcgccc ggaatatccg cgctgtagaa
     1861 gctctcgcct tccagcgtgt tcggatgggt ggaaaagccc gtgagaaccg ccttcacaga
     1921 cccatcagca cgtcgcgcaa agatcacagg aaccgagcca tcccgaggtc cctcgatccc
     1981 gacaaatcct tccgcccagc ttccgaggta catccctgct gagccatcgc ggtggagccc
     2041 ccggcggttc catcccatct gctcgatgaa ccctgttccc gcaaacaggc tggcatcttc
     2101 caggtcttca atcgcccttc caaccgcgcg tgcgaccgct tgctccagcg tttcgaggaa
     2161 ctccgggcag atatccccca cgaggttcgc cgtacacgga gcgacatggg tatgtgttgc
     2221 cgcaatgatg accgattccg gatcgatctc acacgcggat gcacaggccg cgcgcagtgc
     2281 gtccactcgc tccttcggca gcacacacac gtccaccgag accattgcaa cgcgtgtggt
     2341 gccgtcatcc agcaccaccg cattcgccgt cagcgggtcg cgcgcgtgct gaccaaggcg
     2401 gacgtgcatc tgtcccagca ggtggagtgg ccttgagggt gtgaagtctt cccacgcagt
     2461 tccgacgttc atatggcatg gtctccgtgg tgcgaatcag gcctctaccg gcacgtcgtc
     2521 aaggttctca aagaccttcc gtatcgcgtc cgccagcgcg ttaatctgtt cgggcccgtt
     2581 gggtggacga tgattgaaca ctccccaaag acacgtatca atgcactgct gcgaaacggg
     2641 atactggctg agatcgtagg agacgtcctt gcgggcaaac gcgctcgtcc acg
//
LOCUS       DAOSOO010000322         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_37965_length_2687_cov_6.196809, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000322 DAOSOO010000000
VERSION     DAOSOO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37965_length_2687_cov_6.196809"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /locus_tag="PLT86_14420"
     CDS             <1..2427
                     /locus_tag="PLT86_14420"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT37853.1"
                     /translation="WTVEVWDEAGVSVGPSQPAFFSTGLFEETEWQAKWIGRGSPDEP
                     RADTDHFPGEAGEKLKELDIDYRAPLFRREFVLNAPVKRARAFICGLGYHELRVNGEK
                     VGDHLLSPAKTDYRKQVLYETLDLTDALVEGRNAVGITLGNGWFNPLKEWWSWRMQWF
                     GTPRVICQIHVELADGTTQWIVTDEKWRTSTGPILSTCIYSGETYDARLEQPGWDAAG
                     FDDRAWDTANLVAPPGGKLVSAMLPPARVTEIVKAVAVREPSPGVFVFDMGKNFSGWV
                     RLCVQGNAGDEVTLRFAERTYVGGAIDVSNLSLARPTDRYILKGGGVETFEPRFTWHG
                     FRYVSVEGYPGKPTLDAIEGRFIHTDCPPAGKFTCDNDMVMRIHECTVTSQRANIQGL
                     PIDCPQRDERLGWLGDAHISAEQAIHNLDMAALYTKFLRDARANQDERGRISMIVPRP
                     GVEEDLPWTAAYFLIAWYVYLYYGDTRVFEEHREAMERYLDYLATQATGNIQKPAWWG
                     DHLAWHEKWEAASGKPRSISTLFWYVDVLLFSRMNALLGRMEDAEKYRALAADIAEAY
                     NAEYLHTDVPTYDDDTETALALPLFWKLAPPELEPALVDRLLTRLRQHGNHVTTGMIG
                     TKYLVDALAAAGRDDVVWMLVNRKGFPGWHYLLRDDMTSMVEHWGRGDGSYNHPALGS
                     VDAWFYQGLAGIRVDEARPGYEQFSIRPFVPDALVSHVEAEVETIRGTIRSAWRREGD
                     SVRFDISVPANTTATVALPCAGRVSESGTVLFDRQVCGQVDGVTDVHAGNGTVTMEVA
                     AGTYAFVCGG"
BASE COUNT          491 a          820 c          925 g          451 t
ORIGIN      
        1 tggacagtcg aggtatggga cgaagccggc gtgtcggtcg gcccgtccca gcccgcgttc
       61 ttctccaccg gcctcttcga ggaaacggaa tggcaggcga agtggatcgg tcgcggatcc
      121 ccggatgaac cccgcgccga cacggatcac ttccccggag aagcgggcga gaagctcaag
      181 gaactcgata ttgactaccg cgcgccgctt ttccggcgtg agttcgtcct caatgcgccg
      241 gtgaagcgcg cacgggcatt catatgcggg ctcgggtacc acgagcttcg cgtgaacggg
      301 gaaaaggtcg gcgaccacct cctctccccg gcgaaaaccg actaccgcaa acaggtgctc
      361 tacgaaacgc tcgacctgac ggatgcgctg gtcgagggca ggaatgccgt cggaatcacg
      421 ctgggcaacg gctggttcaa cccgctgaag gagtggtgga gctggcgcat gcagtggttc
      481 ggcacgccgc gggtgatctg ccagattcac gtcgaattgg cggacggcac gacgcagtgg
      541 attgtcaccg acgaaaagtg gcgcacgagc accggcccga tcctctccac ctgcatctac
      601 agcggcgaaa cgtacgatgc ccgccttgag cagcccggat gggatgcggc gggcttcgat
      661 gaccgcgcgt gggacacggc caacctcgtc gccccgccgg gtggaaagct ggtctcggcg
      721 atgctgccgc ctgcccgcgt gacggaaatc gtgaaggcgg ttgccgtgcg cgagccgtca
      781 cccggggtat tcgtgttcga catggggaag aacttctccg gctgggtgcg gctttgcgtg
      841 cagggcaacg ccggggacga ggtgacgttg cgtttcgcgg agcggacgta tgtcgggggc
      901 gcgattgatg tctccaacct gagcctcgcc cgtccgacgg accgctacat cctcaagggc
      961 ggtggcgtgg agacgttcga gccgcggttc acctggcacg ggttccggta cgtttccgtg
     1021 gaagggtacc ccggcaagcc cacgctggac gccatcgaag ggcggttcat ccacaccgac
     1081 tgcccgcctg ccgggaaatt cacctgcgat aacgacatgg tgatgaggat tcacgagtgc
     1141 acggtcacct cgcagcgggc gaacattcag ggactgccga ttgactgccc gcagcgggat
     1201 gagcggctcg gttggctggg cgatgcgcac atcagcgccg aacaagcgat tcacaacctc
     1261 gatatggcag cgctctacac caagttcctg cgcgatgcga gggccaatca ggatgaacgg
     1321 ggccggatct ccatgattgt cccgcgcccc ggcgtggaag aggacctgcc ctggacggcg
     1381 gcgtacttcc tcatcgcgtg gtacgtctac ctctactacg gcgatacgcg cgtgttcgag
     1441 gagcaccgcg aggcgatgga gcgctatctc gactacctcg ccacgcaggc gaccgggaat
     1501 atccagaagc ccgcctggtg gggcgaccat ctcgcctggc acgagaagtg ggaggcggca
     1561 agcggcaagc cccgctccat cagtacgctg ttctggtatg tggatgtcct cctgttcagc
     1621 cggatgaacg cactgctcgg caggatggaa gatgcggaga agtaccgcgc gctggccgcc
     1681 gacatcgccg aggcctacaa cgcggaatac ctgcacaccg acgtgccgac gtacgatgac
     1741 gacaccgaga ccgcgctggc gttgccgctc ttctggaagc tcgcgccgcc ggagcttgag
     1801 cctgcgcttg ttgaccggct gttaacacgt ctccggcagc acgggaatca cgtcaccacc
     1861 ggcatgatcg gcacgaagta cctcgtggat gcgctggccg ccgcagggcg ggacgacgtc
     1921 gtctggatgc tggtgaaccg gaagggcttt cccggctggc actacctcct gcgcgacgac
     1981 atgaccagca tggtggagca ctggggccgc ggggatggct cgtacaacca cccggcgctc
     2041 gggtcggtgg acgcctggtt ctatcagggg ctggcgggta tccgggttga tgaggcgcga
     2101 cctggatacg agcagttcag catacgcccg tttgtgccgg atgcactggt gagccacgtc
     2161 gaggcagaag tcgagacgat tcgcggcacg atccgttccg catggcgccg cgagggcgac
     2221 tcggtgcggt tcgatatcag cgtcccggcc aacacgaccg caaccgtggc gctgccctgc
     2281 gcgggccgtg tgagcgaatc cggcacggtg ttgttcgacc ggcaggtctg cggtcaggtg
     2341 gacggcgtaa ccgacgtgca cgcggggaat ggcacggtga cgatggaggt cgccgcgggg
     2401 acgtacgcgt ttgtgtgcgg ggggtaggtg gggtggcata cgacgcatac ggccgatagg
     2461 acctttcggc cgccggggcg atggagggac gcgggccggc agattggctg cagggaacaa
     2521 tgcggtggtt ttctggattc ccgcctgcgc gggaatgacg gctccatttc cgctacagac
     2581 actcaacctt tctgaggacg ctttcggcac cacgcgggat ccaatgagaa cgccccgcgc
     2641 aaaaaacacg gtcattcccg cgcaggcggg aatccatggc tccgaca
//
LOCUS       DAOSOO010000323         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38241_length_2672_cov_3.733282, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000323 DAOSOO010000000
VERSION     DAOSOO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38241_length_2672_cov_3.733282"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2672
                     /locus_tag="PLT86_14425"
     CDS             <1..>2672
                     /locus_tag="PLT86_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37854.1"
                     /translation="IVWDHSTLLSSGLTIIWTHCDVEQASGVVPGTGNINADPLFVDA
                     ANGDFRLMPGSPCIGTGSSGKNMGAWPALSEVETIISSNTIWTKANSPYRITNKVVVD
                     VGATLTIEPGVDVLFDADVQFVVNGRLHAVGTAQDSIRFLPGSAANWRGIRIAGLDSS
                     SIAYARISGANARGDAPENYGGGLYVAGFGARVGAANSVISGNAASYGGGICCHDGGL
                     AALTGCAISNNSATYGGGVANIASILPVELRMEGCVVSGNSASEGGGIYSKSTSTTVA
                     TLMLLDCRVVDNTSNSRGGGIVNQGNLFVERCSISGNAANFGAGLYNKGGGSVALQNC
                     LVFGNAATGAAGGWYNEASASLTMTNCTVTSNSSYSYSGIANFGVASITNSIVYGNPS
                     ASPQFVGGDLAVNYSDVQWVIGVPGGTNIDADPLFVDAANGDYRLQPGSPCIGTGIAG
                     SDMGAFSDHAGQPVISSNTTWTKAASPYRVAESIAVWPGATLTIEPGVDVLFDGHAQL
                     VVWGNLIAVGTATDSIRFLKGSVGAWRGIRIESGDSSVIAYARISGVLADGTTEAAKN
                     GAVWNGGRLTLAHCVLSGNSATSQGGAIWNAQTLFMTDCTVERNSASVGGGVCNSGAL
                     NARNCLFAGNSASSSAGAVSNSGDLTMDNCVLSNNSSQGAGGGIGTTGSLRMANCAAV
                     GNTANGHGGGIWATTSGLTMTNCVVTGNAATADGGGVYLGLGAPATMTNCIFGDNAAK
                     NGGAVACSEERLFMEKCLVIRNAAAEKGGGVLFAVWGVPATFTNCTVSCNTAALSGSG
                     LYVSSNVLVLITNSIVWANSPVEQIFDVKGTVRATYSCLQTGTVWSGVGNINADPLFI
                     DPGTDSMRLHSTSPCRDAGDPASPLDPDGSRADM"
BASE COUNT          536 a          759 c          848 g          529 t
ORIGIN      
        1 catcgtgtgg gaccacagca cccttctgtc ttccgggctt accatcattt ggacccattg
       61 cgatgttgag caggcttcgg gagtggttcc cggtacgggt aacattaacg ccgacccgct
      121 gtttgtggac gcggcgaacg gggatttccg gctgatgccg gggtcgccgt gcatcgggac
      181 gggatcatcg ggcaaaaaca tgggcgcctg gccggcgctt tccgaggtcg agaccatcat
      241 ctcgtccaat acgatatgga cgaaagcgaa cagcccgtac cggataacga acaaggttgt
      301 ggttgacgtc ggcgcgacgc tgactatcga gcccggtgtg gatgtcctct ttgacgcgga
      361 cgtgcagttc gtggtaaatg gcaggcttca cgcagtgggc accgcacagg acagcatccg
      421 gttccttccc ggcagcgccg cgaactggcg gggcatccgt atcgcaggcc tggactcgag
      481 ctccatcgcg tatgcccgca taagcggcgc gaacgcccgg ggagacgcgc cagaaaatta
      541 cggcggcggc ctctacgttg cgggtttcgg cgcgcgagtc ggggcggcca actccgtgat
      601 ctctggaaat gccgccagtt acggcggagg catctgttgc catgacggtg gcctcgctgc
      661 gcttaccggt tgcgcgatct cgaacaattc cgcgacgtat gggggtggag tcgccaatat
      721 cgccagcatt ttgcccgttg aattgagaat ggaagggtgt gtcgtttccg gcaattccgc
      781 atcggaaggc ggaggcattt acagcaagtc gacttcgacg acggttgcca cgctcatgct
      841 cctggactgc agggtcgtgg acaacacgag taacagcaga ggcggaggta tcgtgaacca
      901 gggaaacctg ttcgtggaac ggtgctccat ctctggcaat gcggccaatt tcggagccgg
      961 gctttacaac aaaggcggcg ggtcagtggc cctgcagaat tgcctggtat tcgggaacgc
     1021 cgccacggga gcagcgggtg gttggtacaa cgaagccagc gcctcactga ccatgacgaa
     1081 ttgtaccgtt acaagcaact ccagttactc ctacagcggt atcgcaaatt tcggcgttgc
     1141 gagcatcacg aactctatcg tgtatggaaa cccgtcagcc tcgcctcagt ttgtcggcgg
     1201 cgatctggcg gtgaactact ccgacgtcca gtgggttatc ggtgtcccgg gcggcacgaa
     1261 tattgacgcc gatccgctgt tcgtggacgc agcgaacggg gattaccggc ttcaaccggg
     1321 gtccccatgc atcggtaccg ggatcgcggg cagtgacatg ggcgcattct ccgaccacgc
     1381 tgggcaacca gtcatatcct cgaatacgac gtggacaaaa gccgcaagtc cctaccgggt
     1441 agctgagtcc atcgccgttt ggccgggggc gacgttgacc atagaaccgg gcgtggacgt
     1501 cctttttgac gggcacgcgc agttggttgt ctggggtaac cttatcgccg tcggcacagc
     1561 gacagacagc atccgcttcc tgaaaggttc ggtaggggcc tggcgcggca tacggatcga
     1621 aagcggggat agcagtgtca ttgcctacgc acgcatcagc ggagtgctgg ccgatggaac
     1681 cactgaagcc gcaaaaaacg gcgcggtgtg gaacggaggg cgactaacgc tcgcgcactg
     1741 cgtcctttcc ggcaattccg caacgtccca gggcggcgcg atatggaatg ctcagacgct
     1801 ttttatgacc gattgtactg tcgagcgcaa ttccgcgagc gttggtggcg gcgtttgcaa
     1861 ctcgggcgcc ctgaatgcca ggaactgcct ttttgcaggc aatagtgcga gtagctccgc
     1921 tggcgcggtc tccaattctg gagatctgac gatggacaat tgtgttctct ctaacaattc
     1981 ctcacagggc gcgggcggcg gcattggcac cacagggtcg ctcaggatgg cgaactgtgc
     2041 tgcagtgggc aacacggcga atggccatgg tggagggatc tgggcgacta cgagtgggct
     2101 cacgatgacc aattgcgtcg ttaccgggaa cgcggcaact gcagatggtg gaggagtcta
     2161 cctggggctt ggtgcacccg caacgatgac caactgcatc tttggcgaca acgccgcaaa
     2221 aaacggtggc gctgtggcgt gctctgaaga acgcctattc atggaaaagt gcttggtcat
     2281 ccggaacgcc gctgcagaaa aaggcggcgg agttctcttc gccgtatggg gagtgcctgc
     2341 gaccttcacc aactgcaccg tgagttgcaa cactgcagcc ttgtccggca gcggccttta
     2401 tgtgtcgagt aacgttctcg tgctgatcac gaacagcatc gtctgggcaa acagccccgt
     2461 agaacagata ttcgacgtga aagggaccgt ccgcgctaca tattcctgcc tgcagacggg
     2521 tacggtgtgg tccggagtcg gtaacatcaa cgccgacccc ctgttcatag atccgggaac
     2581 cgacagcatg cgattgcact cgacctcccc atgccgagac gccggtgatc ctgcctcccc
     2641 gctcgaccct gacggctccc gcgcggatat gg
//
LOCUS       DAOSOO010000324         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38385_length_2664_cov_5.099272, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000324 DAOSOO010000000
VERSION     DAOSOO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38385_length_2664_cov_5.099272"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLT86_14430"
     CDS             <1..735
                     /locus_tag="PLT86_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT37855.1"
                     /translation="VHANLAAFEAVLADAGTADEVVYLGDIVGFGPRPAECADLLMRL
                     GARAVPGNHDASMLAIRKRGEMHSNPRDWDEWTFNQLSRAHLDFLDSLPAALEVDFCG
                     VPATVTHHPAGAPYLHPDMPDVVFSAFLEKATRPTLICGHSHREINRMVNGRRYLCIP
                     SIGQPRNGDPRAGYAIERDGTIEFRYVAYDVERTVRETAAIGLDEQFLERWLQFLRTG
                     HDDEWSRDYVPGKPVNKWLDAAIARF"
     gene            complement(943..>2664)
                     /locus_tag="PLT86_14435"
     CDS             complement(943..>2664)
                     /locus_tag="PLT86_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT37856.1"
                     /translation="APPGLRGPMPAQMPVPSSDKFTFAIIGDRWGGEVAAWSVFDRSV
                     EEINLTRPDFVMSVGDLIDGKVTVPAKQDSMWRDFWLHAGKFTAPFFVALGNHDISNP
                     TMRAHWKQNYGPTYYSFDYAGCHFIVLCTEEMVGTTASGSEFLGPEQTAWALQDLAAH
                     AGARHTFVFMHKPIWRLGPNGASAPEIARIEQALNGRPATFFAGHTHTLELRRRGAYR
                     YFVLGPTATRLNDPAGVTPESGSYNHYTTVTVEGNDAHVAIRRPGNVYREDIADPEIM
                     ERLIASVRTEAEPPRGLDGPQATTAMTLQITNPFEHDTLFAHISIDGLSPAGWQSPEA
                     GGGTLPVAPGTTERTRFTFRVPTDRLVPLPELYIEIFHAGRRLGGFTRPISVFTEAMT
                     RRVDEWTVIAPFALGGTPLDSVNVGLLATDPQSAMRGFFRLRGPEGVWNPRATYRDGA
                     TQLRPRTVKTDSGTTRLDLGQAFGRGSNTLAYAVCGIRAPRACTVYALLGANDHADIL
                     VNGTPATGNRVVSLTRGAEYIALPLKAGWNTVVVRSANQGANWWFDFTLFDPTESVRI
                     APVPGRN"
BASE COUNT          439 a          825 c          901 g          499 t
ORIGIN      
        1 gtgcacgcca atctcgcggc gtttgaggcc gtgctggcag acgcgggtac ggcggatgag
       61 gtcgtctatc tcggcgacat cgtcgggttc ggcccgcgcc cggcggagtg cgcggacctg
      121 ctgatgcgtt taggcgcccg cgctgtgccg ggcaaccacg acgcgtccat gctggccatc
      181 cggaagcgcg gggaaatgca ctcgaatccg cgcgactggg acgagtggac gttcaaccaa
      241 ctttcacgag cccaccttga ttttctcgat tcgcttcctg cggctctgga ggtcgatttc
      301 tgtggcgttc cagccacggt gacgcatcac cccgccggcg ctccgtacct ccatccggac
      361 atgccggacg ttgtgttctc tgctttcctc gaaaaggcaa cccgccccac gctcatctgc
      421 ggccactcgc accgcgaaat taaccggatg gttaacgggc gccgatatct ctgtatcccg
      481 tccatcggcc agccgcgaaa cggcgatcct cgcgcaggat acgccattga gcgcgatggg
      541 acgatcgagt ttcgctacgt ggcgtacgac gtggagcgaa cggtccggga aacagccgcg
      601 attggcctcg atgagcagtt ccttgaacgc tggttgcaat tccttcgcac cgggcacgac
      661 gatgagtggt cgcgggacta cgtgccggga aaaccggtga acaagtggct ggatgcggcg
      721 attgcgcggt tttgagaatg gatttcgttg gtcgtctcga tacaacgagc gctgcccttc
      781 gacaggctca gggaccacgc gctcgccact cgacgaccgg gagggggcgg tagcaaactg
      841 cctgtcggtc tccgagtggc gtgaggacgt agtccgaacg ctgtatcgag gagtccgaca
      901 cgcgatggga gcgggtcagc taccccttct ctccctatcc gcctagttcc tccccggcac
      961 gggggcgatt cggaccgact ccgtcgggtc gaacagggtg aagtcgaacc accagttcgc
     1021 tccctgattg gccgaccgta cgacaacggt gttccaccct gccttcaagg gtagcgcgat
     1081 gtattcggct ccgcgggtga ggctgaccac ccggttgccc gtggccggag tgccgttcac
     1141 gaggatgtcc gcgtggtcgt tcgcaccgag gagggcgtac acggtgcagg ctcgcggggc
     1201 ccgaatgccg cagacggcgt acgccagcgt gttggacccc ctgccgaacg cttgtccgag
     1261 gtcgagccgc gtggtgccgc tgtcggtttt caccgttcgc gggcggagct gcgttgcgcc
     1321 gtcgcggtag gtcgcccgcg ggttccagac gccctccggg ccgcgcaggc ggaagaagcc
     1381 acgcattgct gactgtggat ccgtggccag gagcccgaca ttcacgctgt cgagaggggt
     1441 tccgccgagg gcaaacggtg cgatcaccgt ccactcatcc acgcggcgag tcattgcctc
     1501 ggtgaacacg ctgatcgggc gcgtgaaccc gcccagcctg cggccggcat ggaatatctc
     1561 gatgtacagc tccggcagcg gcaccagccg gtctgttggc acgcggaagg tgaacctcgt
     1621 ccgctcagtc gttccgggag caacggggag ggttccgccg cccgcttccg gggattgcca
     1681 gcccgcggga gacaggccgt caatgctgat gtgcgcgaaa agggtgtcgt gctcaaacgg
     1741 gttcgtgatt tgcagggtca ttgcggtggt cgcctgcggc ccatccagtc cccgcggcgg
     1801 ttcggcttcc gtgcgaacgc tggcgatgag cctctccatg atctccggat cggcgatgtc
     1861 ctcgcggtac acattgcccg gccggcggat cgccacgtgt gcgtcgttgc cctctaccgt
     1921 cacggtcgtg taatggttgt acgaacccga ttccggggtt actccggcgg ggtcgttcag
     1981 acgggtggcc gtggggccga ggacgaagta gcggtacgcg ccccttcttc gaagctccag
     2041 cgtgtgcgtg tggccagcga agaaggtagc cgggcggccg ttgagcgcct gctcgatgcg
     2101 tgcaatttca ggggcggatg cgccgttcgg gccgagccgc cagatcggtt tgtgcatgaa
     2161 gacgaacgtg tgccgggcac cggcgtgtgc cgcaaggtct tgcagcgccc aggcggtttg
     2221 ttccgggccg aggaactccg agccggaagc cgtggtgcct accatctcct cggtgcacag
     2281 cacaatgaag tggcagccag cgtagtcgaa ggagtaatac gtaggcccgt agttctgttt
     2341 ccagtgggcc cgcatcgtcg ggttggagat gtcgtggttt cccagcgcga cgaagaacgg
     2401 cgcggtgaac ttgcccgcgt gaagccagaa atcgcgccac atggagtcct gtttcgccgg
     2461 aacagtcact ttgccgtcta tcaggtcgcc gaccgacatg acgaaatcgg gccgcgtgag
     2521 gttgatctcc tccactgatc ggtcgaacac ggaccacgcg gctacttcac caccccagcg
     2581 gtcgccgatg atggcgaagg tgaacttgtc ggatgacggg acgggcattt gtgccggcat
     2641 cgggccacgc aggccgggag gcgc
//
LOCUS       DAOSOO010000325         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38401_length_2663_cov_3.975844, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000325 DAOSOO010000000
VERSION     DAOSOO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38401_length_2663_cov_3.975844"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLT86_14440"
     CDS             complement(<1..391)
                     /locus_tag="PLT86_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37857.1"
                     /translation="MCRDGFHIERVAFGATDDATDIGISPARLAFEPGSRLPSKLPAN
                     VLFSKGHQPPVLRNGEMIAQIHPLVEFLNAHLGDNRLEPRTDYTVHNSGSAVAYLFAD
                     TSVPLTSLLAGSGFRINLLNMVKSARAQ"
     gene            complement(645..776)
                     /locus_tag="PLT86_14445"
     CDS             complement(645..776)
                     /locus_tag="PLT86_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37858.1"
                     /translation="MRDIARLPADDRSDLFRLTAQAMNVHEAATANDTVPTRGWSEV"
     gene            complement(769..1389)
                     /locus_tag="PLT86_14450"
     CDS             complement(769..1389)
                     /locus_tag="PLT86_14450"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOT37859.1"
                     /translation="MAKNATGKIVAKVCDGGRGKAFTPKDFLELTSHETARQALARLA
                     KEGTVRRVMRGVYDYPRYSSFLNAPSPADPDAIAHAIARNHGWSIVPTGDTALNLLGV
                     STHVPAQWVYLSDGPAKQYEWEGGKITFKHRTNKETSRLSPKTALVVQALKRLGKERV
                     DESVVHALRSKLDAKDRARAAREARYATSWVYEVIRQLAEGKAPDA"
     gene            complement(1490..2206)
                     /locus_tag="PLT86_14455"
     CDS             complement(1490..2206)
                     /locus_tag="PLT86_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37860.1"
                     /translation="MAVNVRAGPDSNWFVWWEDENAPSSLAAERLFAALLEHRSIATA
                     ADSEGLNQAKLWEFTVTRDFHNGLGGVIAALEAAAEFGIEAVWNRAMELKQTAEAVTD
                     AARYDGVFREKALLGGKEILAALTEEGRTLERAFVCRSENLPDRTKVASWFQRVGTAV
                     RRMEQAGMDVDGVMLVIEGEQRYFLSSPRAVVPSVLCTIDQIRRLLEDADDHWREMRN
                     ANDEADEAEGNRFNLWSCWL"
     gene            complement(2280..>2663)
                     /locus_tag="PLT86_14460"
     CDS             complement(2280..>2663)
                     /locus_tag="PLT86_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT37861.1"
                     /translation="FSLRQKVTNHLSFFLVENVPVPRLDSSAAWFWPVVSRAARLICT
                     TPEFDDLAREVGLAGHEDGATTPAERARLRAELDGIVAHIYGLTEEEFAHILRAFPLV
                     PEATKTAALEAYRDVAAGRITPERY"
BASE COUNT          493 a          837 c          758 g          575 t
ORIGIN      
        1 cctgtgcgcg ggcgcttttc accatgttga ggaggttgat tctgaacccc gatcccgcca
       61 ggagggaagt gaggggaacg gatgtatcgg cgaaaaggta cgcgacggcg gagccagaat
      121 tgtgcacagt gtagtcggtt cgaggttcca ggcggttgtc gccgagatgg gcgttcaaga
      181 actcgacgag gggatggatt tgagcaatca tttctccgtt gcggagaacg ggtggttgat
      241 gccccttgct gaacagcacg ttcgccggca gtttcgaagg gagtctgctt cccggttcga
      301 acgccaggcg cgcgggtgag attccgatgt cggttgcgtc atcggtggcc ccaaatgcga
      361 ctctctcgat atggaatccg tccctgcaca cgacctcgct tcccggagcg cgcaccgagg
      421 gccggcgcgg gtcccataca cgcaggtggt gggttgtcgc gtcaaacagg taggggcccg
      481 gtagttcgaa gcaagcggaa agaactcgct tccactcgtc gtgttcgtcg gccccgttca
      541 ggcggtaaca ttcccaagcg acctgttttt cggctattgt gtgcctcccg cggcctctgc
      601 acgacgagat gcctgcatgc ggcctgaaga actgcaataa atggtcacac ctcggaccaa
      661 ccacgcgttg gcacggtatc gttcgcagtt gccgcctcgt ggacgttcat cgcctgcgcc
      721 gtcagacgga acagatcact gcggtcgtcc gcgggaagcc gtgcaatgtc acgcatcggg
      781 ggccttgccc tccgccagtt gccggatcac ctcatacacc catgacgtgg catatcgggc
      841 ctctctggcg gcgcgtgccc ggtccttggc atcgagcttc gagcggagcg cgtgcacaac
      901 cgactcgtcc acacgctcct ttccgagcct tttcagcgct tgcaccacaa gagcagtttt
      961 gggagagagc ctggaggtct ctttgttcgt ccggtgtttg aacgtgatct tgcccccttc
     1021 ccattcatac tgtttcgcag ggccatcgct gaggtatacc cattgcgcag gcacgtgcgt
     1081 ggatactccg agaagattga gcgcggtgtc cccggtcgga acgatactcc atccgtggtt
     1141 gcgggcgatg gcgtgggcga tggcatcggg gtctgccggc gacggtgcgt tcaggaacga
     1201 gctgtatcgc gggtaatcat acacgccccg catcacccgc cggaccgtgc cctcttttgc
     1261 cagtcgcgca agagcctggc gcgcggtttc atggcttgta agttcgagaa agtctttcgg
     1321 agtgaaggct ttgcccctcc ctccgtcgca aaccttcgca actatcttac cagtagcatt
     1381 ttttgccata tttggatcat cctctttgtc acgaaaaata gctattattc gtgacaaagg
     1441 caaatgcact tatgtggtat ctcgatgctt ccggcaacgt acgggcatct caaagccaac
     1501 aggaccagag attgaaccgg tttccctctg cttcatctgc ttcgtcgtta gcgttccgca
     1561 tttccctcca gtgatcgtcc gcatcttcga gtaaccgccg aatctggtca atcgtgcaga
     1621 gaacagacgg gacaaccgcc cgcggggagg acagaaagta gcgctgttcg ccttcaatca
     1681 ccagcattac cccgtcaacg tccatgcctg cttgttccat ccgtcggacc gcggtaccca
     1741 ccctttggaa ccacgacgcg accttcgtcc tgtccgggag gttttccgac ctgcacacga
     1801 aggctctttc cagcgtccgt ccctcctcag tcagcgcagc gagaatctct ttccccccca
     1861 acaaggcctt ctcgcggaac actccgtcgt atctcgccgc atccgtgacc gcttccgccg
     1921 tttgcttcaa ttccatcgcc cggttccaca ccgcctcgat gccgaattcc gccgcggcct
     1981 ccaatgcggc gatgactccg ccgagtccgt tgtggaaatc gcgcgttacg gtaaactccc
     2041 agagtttcgc ctggttgagg ccttcactat cggctgcggt tgcgatggaa cggtgttcca
     2101 agagagcggc aaataggcgt tcggcagcca gggaagaggg cgcgttctca tcctcccacc
     2161 acacgaacca gttgctgtca ggtcccgccc tcacattgac tgccatcgcg tgctcctcat
     2221 gcacaacccg tagattcccg cccttactcc ccctcttccg cgtccgcgcc catgcccgat
     2281 caatacctct cgggcgtgat cctccccgcc gccacatctc ggtacgcttc cagcgcggcc
     2341 gttttcgttg cttccggcac gaggggaaac gcgcggagga tgtgcgcgaa ctcctcctcg
     2401 gtgaggccgt agatatgggc aacaatgccg tccagttccg cccgcaggcg agcgcgttcg
     2461 gcaggggtgg ttgcgccgtc ttcgtgaccc gccagaccca cctcccgcgc cagatcgtca
     2521 aattccggcg ttgtgcagat caaccgggcg gcccgggaaa caaccggcca gaaccacgca
     2581 gcagacgagt cgagacgggg tacgggtacg ttttcgacga gaaagaagga cagatgattt
     2641 gtgactttct gtctcaatga aaa
//
LOCUS       DAOSOO010000326         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38587_length_2653_cov_4.364896, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000326 DAOSOO010000000
VERSION     DAOSOO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38587_length_2653_cov_4.364896"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLT86_14465"
     CDS             complement(<1..900)
                     /locus_tag="PLT86_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37862.1"
                     /translation="MTQYERVRAALLGEPADRPPVIGGWVCQEDFLVTMSHVTRDEFW
                     KNPYAHATTAYKNAGADALVQFVLPKRETEATQGEYGRPTNFGKQEERAEDRFPTLES
                     VIEWVRKQPTVDEVRRTFDAKKAYDQYLEVVHERDDIAPMVWIPGHVCGCPPFMWYSY
                     LGYENYFAGMMEAPEVFERFFAMEGEQRRLQNAEVARAIRENGLLPLLYFGEDICYSS
                     GPMCSPKLLREIYFPHLARAMEPLVEAEIISVWHSDGNIMPIIDDLIRCGVDGFQGLE
                     EDHGMSLETLAGMTNRNGVPLLLW"
     gene            complement(1024..1998)
                     /locus_tag="PLT86_14470"
     CDS             complement(1024..1998)
                     /locus_tag="PLT86_14470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37863.1"
                     /translation="MPLPRKYTLANKLFTASRYPFRRLGGYPTLVNLEVTKKCNARCD
                     FCDYWKTESEERLSDYAPVIARLKPTVVMLTGGEPLLRRDLEAVIAGIKKQSPAVYLG
                     LITHAAMLTPKRGLALWEAGLDQLSISLDFLDERHDKARGIPGLVKKVTETIPLLVEA
                     GIDNLALNTVIKSDNLDQVVAMVHQCEDWGIGIAFSAYADVKVGNKEHNIRDAQMREL
                     QRVIDELLRLKREGAPILSSSYYLSRVPEFFATGHIGGCVAGDRFVTVTPSGHVKRCS
                     EFDAEVHFSEWTPKSIGTTDCDVCWFSCRGESQAPVISLERLKDTLLK"
     gene            complement(2043..>2653)
                     /locus_tag="PLT86_14475"
     CDS             complement(2043..>2653)
                     /locus_tag="PLT86_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37864.1"
                     /translation="RVQDEEVVRDFGALLEALLARREVGIIATQRSDGAVVVRSRSGT
                     GVVTPLGEGHFQYSTQGGDPFGYRGLEGVWNADDLLEETLATRYPDGPVQLEQLFHSP
                     RTGDIVVSAEVGFDLRDKHENPEHFSGHGALHADHMVVPWFSTVPLPDAPLRTVDVFP
                     TILHWMRKPIPPGVTGKDRLASSLEPKQAELAGTRSVEASAF"
BASE COUNT          515 a          904 c          702 g          532 t
ORIGIN      
        1 ccagagcaac agcggtacac cgttccggtt cgtcatcccg gccaacgttt caaggctcat
       61 tccgtggtct tcttccagcc cctgaaaccc gtctactccg catctgataa ggtcatctat
      121 gatcggcatg atgttgccgt ctgagtgcca caccgaaatg atctccgcct ccacaagcgg
      181 ttccatcgcg cgtgcaaggt gcggaaaata aatctcccgc agaagcttcg ggctgcacat
      241 cgggccgctg ctgtagcaga tatcctcccc aaaatagagg agcggaagta acccgttctc
      301 ccgtatcgcc cgtgccacct ccgcgttctg gagccgccgt tgctcccctt ccatcgcgaa
      361 aaatcgctcg aacacctcgg gagcttccat catcccggcg aaatagttct catatcccag
      421 atacgaatac cacatgaacg gtgggcaccc gcacacgtgc ccgggtatcc acaccatcgg
      481 cgcaatgtcg tcccgctcgt ggacgacctc gaggtactgg tcatacgcct tcttcgcgtc
      541 aaacgtgcgc cgcacctcgt caacggtcgg ttgcttccga acccactcga tcaccgattc
      601 aagcgtggga aatcggtctt cggcccgttc ctcctgtttc ccgaagttgg taggccgccc
      661 gtactcacct tgcgtcgcct cggtctcccg tttcggaagc acaaactgca caagcgcatc
      721 tgcacccgcg ttcttgtacg cggtcgtggc gtgcgcgtaa gggttcttcc agaactcatc
      781 gcgcgtcacg tgactcatgg tgacgagaaa gtcttcctga catacccagc cgccaattac
      841 gggagggcgg tctgcaggtt ctccgagaag ggccgcgcgc acgcgctcgt actgggtcat
      901 cgatgcctcg cacgaaatga ggtggctcac cggttgcggt aaaggaataa acgtagaaag
      961 gagagggatg ggcaaacagc cgggtggagc gcagaaccgg attcggtctt cggcgtgggt
     1021 gcgctatttg agaagcgtgt ctttcagccg ctccagggat atcaccggcg cctgagattc
     1081 tccgcggcaa ctgaaccagc agacgtcgca atccgtcgtc ccgatggact tcggagtcca
     1141 ttcggagaag tggacctccg cgtcaaattc actgcatcgc ttcacatggc cgctcggcgt
     1201 tacggtgaca aaccgatccc cggcaacgca cccgccgatg tgccccgtgg cgaaaaactc
     1261 cggcacgcgg gaaaggtagt acgacgacga cagtatcggc gcaccctctc ttttcaatcg
     1321 caacagctca tcaataaccc gttgcagttc cctcatctgc gcgtcacgga tgttgtgttc
     1381 cttgttgccg accttcacgt cagcatacgc gctgaacgcg atcccgattc cccagtcctc
     1441 gcattgatgc accatcgcca cgacctgatc aaggttgtcc gacttgatta ccgtgttcaa
     1501 cgcaaggtta tcaattcccg cctcaacaag aagcgggatc gtctccgtca cttttttcac
     1561 cagccccggt attccgcgcg ccttgtcgtg ccgctcatcg aggaagtcga gactgatcga
     1621 aagttgatcc agccccgcct cccagagcgc cagaccccgt ttcggcgtca gcatcgccgc
     1681 gtgggtgatc aggccgaggt acaccgccgg cgactgtttc tttatcccgg ctattaccgc
     1741 ctccaggtcg cgccgaagca acggctcccc gccggtaagc atcaccaccg tcggtttcag
     1801 tcttgcgatt accggagcgt aatcgctgag ccgctcctcc gactccgtct tccagtaatc
     1861 gcagaaatcg caccgcgcgt tgcacttctt cgtcacttcc agattgacga gcgtcggata
     1921 tcccccaagc cgccgaaacg ggtaccggga ggcagtgaaa agcttgttcg ccaacgtgta
     1981 ttttctggga agcggcataa agtcctcatg tgtagtggtc ggcaccggtg tcccggttcc
     2041 cgtcaaaagg cgctcgcctc aactgaccgg gtgcccgcca attccgcctg tttcggctcg
     2101 agggaggaag caaggcgatc cttccccgtc acccccggcg gaatcggctt tctcatccag
     2161 tgcagaatcg tcggaaacac gtccaccgtc cggagcggcg catccggcag aggcaccgtc
     2221 gaaaaccacg gcaccaccat gtggtccgcg tgaagcgctc cgtgcccgga aaaatgctcc
     2281 ggattctcgt gcttgtcgcg cagatcgaac ccgacctccg cgctcacgac gatatccccc
     2341 gttcgcggcg agtggaaaag ctgctccagt tgtaccggcc cgtccggata tcgtgtcgcc
     2401 agcgtctcct cgagcaggtc atctgcattc cacacgccct cgaggccgcg atacccgaac
     2461 gggtcgccgc cctgcgtcga atactggaaa tgcccctccc cgagcggcgt caccacgccc
     2521 gtgcccgacc gcgagcgcac caccaccgcg ccgtcgctcc gctgcgtggc aatgattccg
     2581 acctcccgcc ttgccagcag cgcctccagc agcgccccga agtcccgcac cacttcctcg
     2641 tcctgcacgc gtt
//
LOCUS       DAOSOO010000327         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38630_length_2651_cov_5.302773, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000327 DAOSOO010000000
VERSION     DAOSOO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38630_length_2651_cov_5.302773"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLT86_14480"
     CDS             complement(<1..717)
                     /locus_tag="PLT86_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOT37865.1"
                     /translation="MKKKGNTMGMTITEKIFAAHCGKKSVVPGELVNAKLDLVMCHDV
                     TTPPAVEMLEKRGMDRVWDPDRVVVTPDHFVPAKDILSAELHRSLDRWVKRHRIRHYY
                     PLGRHGVCHAILPEKGHILPGEVIVGGDSHTCTYGAFGAFSAGIGSTDLAAALYAGEL
                     WFRVPETMKFTLNGVPPEGVYSKDIILEIIRRIGVDGARYMAMEYDGSTLAHLSMEAR
                     MTITNMAIEAGAKSGIIPADH"
     gene            complement(760..2322)
                     /locus_tag="PLT86_14485"
     CDS             complement(760..2322)
                     /locus_tag="PLT86_14485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT37866.1"
                     /translation="MSDDSSVVRIFDTTLRDGEQSPGASLSVQEKIDIAHQLKRLGVD
                     IIEAGFPVSSPAQFEAVERISEEVDGPTIAAFARAVPLDIETAARAVKPAKKKRIHTF
                     IATSPIHMERKLKKTPAEVLTLTGEAVRLAKSFCDDVEFSPEDAGRSEWPFLVEVLTA
                     AIEAGATTLNIPDTVGYMMPDEYGRMIAHLIAHVPGSENVIYSTHCHNDLGLAVANSI
                     AGVVNGARQVECTINGLGERAGNAAMEEFVMALRTRQDVFRLRTNIVTTEITRTSRMV
                     ARCTGLPVQANKAIVGANAFAHESGIHQDGVLKARQTYEIMTPESVGLDSTRLVLGRH
                     SGRHGFKARLEKLGYRLDKDALERAYERFLAIADRKKEVFDEDLAAIVGDEFRDVPEV
                     YKLDYMHCISGSKILPSATVRIIVNGEVHQESEWGDGPVDAVFRAISKVSGFAITLDN
                     YTIRAVTAGTDAIGEVTLRVRDNGHTVTGRGASTDIVEASARAYVDGLNKLAAWHARV
                     AEQDGNQLGETV"
BASE COUNT          434 a          844 c          792 g          581 t
ORIGIN      
        1 gtggtccgcc ggaatgatac ccgatttcgc gcccgcctcg atcgccatgt tggtgatggt
       61 catgcgggct tccatgctca ggtgggcaag cgtgcttccg tcgtactcca tcgccatgta
      121 gcgcgcacca tccaccccga tgcggcggat gatctcgagg atgatgtcct tggaatacac
      181 gccttccgga ggcactccgt tcagcgtgaa cttcattgtt tcgggcacgc ggaaccagag
      241 ctcacccgca tacagcgcgg cggcgaggtc cgtgctgccg attccggcgg aaaacgcacc
      301 gaacgcgccg tacgtgcagg tgtgcgaatc ccccccgacg atgacttcgc ccggaaggat
      361 atgccccttt tcgggcagaa tggcatggca gacgccgtgg cggccgaggg gatagtagtg
      421 cctgatccgg tgccgtttca cccaccggtc gaggctcctg tgcaactccg cgctcaggat
      481 gtcctttgcc ggcacgaaat ggtccggcgt cacaacgact ctgtccgggt cccacaccct
      541 gtccatgccg cgtttctcaa gcatctccac cgcaggcggg gtggtgacgt cgtggcacat
      601 gacaagatcg agtttcgcgt tgaccagttc gcccggaacc acgcttttct tgccacagtg
      661 tgcggcaaat atcttttcag taatcgtcat tcccatggtg ttacccttct tcttcattcc
      721 ccccgcaact ctgcggagat atccggcggc gtagtgaaat cagaccgttt ctccgagctg
      781 gttgccatcc tgttcggcca ctcgggcatg ccatgcggca agcttgttga gcccgtccac
      841 atacgcccgc gcggaggctt ccacgatgtc ggtgctggca ccccgcccgg tgaccgtatg
      901 gccgttgtcc cgcacgcgca gggttacctc gccgatggcg tccgttcccg ccgttaccgc
      961 gcggatggtg tagttatcca gcgtgatggc aaaaccggat accttggaga tcgcgcgaaa
     1021 tacggcgtcc accggcccat cgccccattc cgattcctga tgcacctcgc cgttcacgat
     1081 aatccgcacg gtcgccgagg gcaggatttt gcttccgctg atgcagtgca tgtaatcgag
     1141 tttgtacacc tcgggcacgt cgcggaactc atcgcccacg atggcggcca ggtcctcgtc
     1201 gaacacttcc ttttttcggt cggcgatggc gaggaaccgc tcgtacgcgc gttccagagc
     1261 gtctttgtcc agccggtacc cgagtttctc cagccgcgct ttgaaaccgt gccgtcctga
     1321 atgtcgcccc agcaccagtc gggttgaatc gaggccaacg gactccggcg tcatgatttc
     1381 gtacgtctgc cgcgccttga ggacgccatc ctgatgaatg ccggactcgt gtgcgaaggc
     1441 gttcgcgccc acaatcgcct tgttggcctg caccggaagg cccgtgcacc gggcaaccat
     1501 gcggctcgtc cgggtgattt cggtagtaac gatgttcgta cggagcctga acacgtcctg
     1561 ccgggtgcgc agcgccatga cgaactcttc cattgcggcg ttgccggcgc gttcgccgag
     1621 gccgttgatg gtgcattcga cctgtcgcgc gccgttcacc acgccggcga tgctgttggc
     1681 aaccgcaagg ccgaggtcgt tgtggcagtg agtgctgtag atcacgttct cgctgcccgg
     1741 gacgtgcgcg atgagatggg cgatcatccg cccgtactcg tccggcatca tgtagccgac
     1801 ggtgtcggga atgttgagcg tcgtcgcgcc ggcttcgatg gctgcggtca gcacctccac
     1861 cagaaacggc cattcgctgc ggccggcatc ctccggtgag aactccacgt cgtcgcagaa
     1921 cgatttcgcc agccgtaccg cctcgccggt aagtgtgagc acctcggcgg gtgtcttctt
     1981 gagcttgcgc tccatatgaa tcggactggt ggcgatgaag gtgtgaatcc gtttcttctt
     2041 cgccggcttc accgcccggg ctgccgtttc aatatccagc ggcaccgcac gtgcgaacgc
     2101 ggcgatggtg gggccgtcga cctcttcgga gatccgttcc accgcttcga actgcgcagg
     2161 gctgctgacg gggaaccccg cttcgatgat gtctacaccc agccgcttga gctggtgcgc
     2221 gatatcgatc ttttcctgga cactcagcga tgcgccggga gattgctcgc catcgcgcag
     2281 cgtggtgtcg aatatgcgga ctactgatga gtcgtcactc atggtgctgt ctcgtttccc
     2341 gtcggcatgc ggttgttcgt ggctcgggaa agagacgctg ccgacgagtg cgctcttccc
     2401 cgcataaggg acagaaggtc gctgagatcg gcgaggccgt cgtccatttc ccatcaccac
     2461 ctttcgccat caccaccggg ccggtttttg tgcgccggtg gaaggatgtg agctgccggg
     2521 ttgcggttct atgggtccta tacgttgtat gcgaacgatg ggaaccgttc cgtcctactt
     2581 atccgacaaa aacttctccg tgccgccgcc ttcgagtttc agcgcgccct tcatcatttc
     2641 gcgcaacggt g
//
LOCUS       DAOSOO010000328         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38699_length_2648_cov_2.996915, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000328 DAOSOO010000000
VERSION     DAOSOO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38699_length_2648_cov_2.996915"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PLT86_14490"
     CDS             complement(<1..1328)
                     /locus_tag="PLT86_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37867.1"
                     /translation="MNIQVNFGGGAFSSYYNINRIIGTPSIEMRIAGRSTASCQVVAD
                     SAIVAPSTFAIGTLCNIQNFTNQPGNDGLHLVSSSAGDTTQTALIVGTDQADALQYET
                     VALNGTTPVNSTNTDWKMIHTVTLSAACAGVVTVQEASGDQTIVTIPVGTVHVSDKLF
                     SGFLDSVTRRKVVENSTSTEMLYDCSFVDVAQVLERRICLRDWSADTIGGVIDDILSD
                     DLTDEGFYRSWGGVDCVDDVVSAVEVKNLVGAYKTYAAILNELAQLAGCVWWVDPYRV
                     IHMTALTGSPTPAPTPAPFTISESAPVRNLQLATTKGEYRNREYFVYDEVWATNKTVT
                     LYGDGGASSWVVNPAIYHIADAAVVCDSLERRVRVDSEKDILVKSSAGADFNNEDRER
                     TDEWNIGVVSDNPSDTHRIVMCYWTDADDVLISGQVQLNGTTRVYMGSFD"
     gene            complement(1325..2578)
                     /locus_tag="PLT86_14495"
     CDS             complement(1325..2578)
                     /locus_tag="PLT86_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37868.1"
                     /translation="MRQSLRFPMIMMIGKLIAAYNALKVAGLGAFAVGGPIAIGVAAL
                     AGLIALLTKRWLEKAALGAFNNEASATVIAMNGMTEGQQRLNLAFREGLLARYNEEYA
                     SLPSRASEAESFQEWERAYSRRRQLELLIQQNEREVGAIKGVIGQRGAGAGGGAMPFT
                     LPAGGAAPEMTAAQKREAEAMMERARLEAYTAYARSQIAGVHVSKAPGIWGLERWSAK
                     PEPERVLTEREKRFRVARVLQDETVEKMEVTGAAAERFQSQFDGYVGSFTNWTNGFLE
                     ISDRMGNIASSIAMNWQNAGQIITNALRQMVAQLVGAIAKAMVFKALMTMFGIPTGGK
                     GDLTFGGIIKSAFGFAEGGIVTKPTLALIGEKREPEAVIPLSKMGQFGGGTQTINIVL
                     DGQVIASAVSNRQAKEIRMRGVLAA"
BASE COUNT          518 a          833 c          676 g          621 t
ORIGIN      
        1 caatcgaatg accccatgta tacccgcgtt gttccattca gttgcacttg cccagaaata
       61 agaacgtcgt ccgcgtctgt ccagtagcac atgacgattc ggtgcgtatc ggaagggttg
      121 tcagagacga ctccgatgtt ccattcgtcc gttcgttctc tgtcttcatt gttgaaatcc
      181 gccccggcag atgactttac caagatgtcc ttctcggagt ccacgcggac tctccgctct
      241 agggaatcac acaccactgc cgcgtctgcg atatggtaaa ttgcgggatt gactacccat
      301 gatgacgctc caccatctcc gtaaagggtt acggtcttat ttgtggccca cacctcatcg
      361 tatacgaagt actcccggtt ccggtattcc cctttggttg tcgccagctg taggttacgc
      421 acgggagcgg actcggaaat ggtgaacggt gccggtgttg gtgcgggcgt tggcgagccg
      481 gtcagggccg tcatgtgaat cacgcggtag ggatctaccc accaaacaca ccctgcgagc
      541 tgcgcgagtt cgttcaagat cgcggcgtat gtcttgtatg ccccaacgag gtttttcact
      601 tctaccgcgg aaaccacgtc gtcaacgcag tcaacgccgc cccatgaacg gtaaaacccc
      661 tcatcggtca gatcgtcgct cagtatatcg tctatcacgc cgccgatggt atctgcgctc
      721 caatcccgca ggcatatacg ccgctccaga acctgcgcaa cgtcaacgaa cgaacagtcg
      781 tacagcattt cagttgacgt gctgttttcc acaaccttgc gtcgcgttac tgaatcaagg
      841 aacccagaaa acagcttgtc ggacacatgg acagttccaa ccgggatcgt gacgattgtc
      901 tgatcgccgc tcgcttcctg caccgtgact acacccgcgc atgcggcgga cagcgtgacc
      961 gtgtgaatca tcttccagtc ggtgttcgtc gagttgaccg gcgtcgttcc gttcagtgcg
     1021 actgtttcat attggagggc gtctgcttgg tcagtcccga cgatcaaggc cgtctgcgtt
     1081 gtgtcccctg cggaggaaga tacaaggtgc agcccgtcat tacccggctg attcgtgaag
     1141 ttttgaatgt tgcacagcgt tccgatagcg aacgtcgaag gcgccacgat tgccgagtcg
     1201 gccaccacct ggcaggaggc ggttgagcgg cctgcaatcc gcatttcaat cgagggcgtg
     1261 ccgatgatgc ggttgatgtt gtagtatgag gaaaaggccc ccccgccaaa gttaacctga
     1321 atgttcatgc ggccaatacc ccgcgcatcc ggatttcctt agcctgccgg ttcgacacag
     1381 cggacgcgat cacctgcccg tccaatacga tgttgatggt ctgagtgccg ccgccaaact
     1441 gccccatctt tgacaatggt atcacggcct ctggttcgcg cttctccccg atcagggcaa
     1501 gcgttggctt agtgacaata ccgccctcag cgaaaccgaa cgcgcttttg atgatcccgc
     1561 cgaatgtcaa atcgcccttt ccgcccgtcg gaatcccaaa cattgtcatg agcgccttga
     1621 acaccatcgc cttcgcaatc gccccgacga gttgagccac catctgccgg agggcgtttg
     1681 tgataatctg cccggcgttc tgccagttca tggcaatcga cgaggcgata ttccccatcc
     1741 ggtcacttat ctccaggaac ccgttggtcc agttggtgaa cgagcccaca tacccgtcga
     1801 attgcgactg gaaccgttca gcagctgcgc ccgtaacctc catcttttcc actgtttcat
     1861 cttggaggac tcgtgcgacc ctgaaccgct tctctcgttc ggttaaaacc cgttctggtt
     1921 ctggctttgc gctccaccgt tccagtcccc agatacccgg cgccttgctc acgtgcacgc
     1981 ctgcaatctg agaacgagca tatgccgtgt aggcttctaa ccgcgcgcgt tccatcatgg
     2041 cttcggcttc tcgtttttgc gcagccgtca tttccggagc cgcgccgcca gctggcaagg
     2101 tgaacggcat cgccccacct ccagcacctg ccccgcgttg gcctattacg ccctttatcg
     2161 cgccaacttc tctctcattt tgctggataa gtaactcgag ttgacggcgg cgactgtatg
     2221 cccgttccca ttcctgaaaa ctctcagctt cggaggccct gctgggcagg ctggcgtact
     2281 cctcgttgta tcgcgccaaa agcccctctc gaaacgcgag attgagccgc tgttgtcctt
     2341 ccgtcatacc attcatggcg atcacagtcg ctgacgcctc gttattgaac gccccgagtg
     2401 ccgccttctc cagccatcgc ttggtcagca gggcaatcaa gcccgcgagt gccgcaacgc
     2461 ctatcgctat cgggccgcca acggcgaacg caccaagtcc agccaccttc agcgcgttgt
     2521 atgccgcgat aagcttgccg atcatcatta tcatcgggaa tcgcagcgat tgccgcatac
     2581 atcgcaattg tggcgttctt ctcgtgtata ttcatcgcgg cgaaccggtc actcacttcc
     2641 tccagcga
//
LOCUS       DAOSOO010000329         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_38847_length_2639_cov_4.949690, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000329 DAOSOO010000000
VERSION     DAOSOO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38847_length_2639_cov_4.949690"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1274)
                     /locus_tag="PLT86_14500"
     CDS             complement(57..1274)
                     /locus_tag="PLT86_14500"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT37869.1"
                     /translation="MPATKKPTANAKPSTASTPAAERWSTPWDMTRLSATPAWELDPT
                     RSTKDMKAVFYEGVPFRGKPTKVFAYYAIPKVKKGEKVPAMVLIHGGGGSAFIPWVKL
                     WLDRGYAAISMDCCGCTNDGDYGDPHPRHEHGGPAGWGGFDALDEPVEDQWTYHAVAA
                     AILGHSLIRSFPEVDAERTGLTGVSWGGYTTCIVAGVDQRFKFAAPVYGCGFLGLNSA
                     WLETFANMGKEKAEKWLRMWDPSVYLPGATMPMVWVTGTNDFAYPMDALQLSYRLPKD
                     GRTLAVRVRMEHAHGPAGEAPPEILAMAEHLFRGKPALARITKQGVRCGRSSVEFESE
                     VPVVAAEFNWTTDTGNWMTRNWETVPASLDAKAGKASAKVPAGATGHYINLVDERGCV
                     VSSEHVAVEKKGK"
     gene            1437..2507
                     /gene="epmA"
                     /locus_tag="PLT86_14505"
     CDS             1437..2507
                     /gene="epmA"
                     /locus_tag="PLT86_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOT37870.1"
                     /translation="MSTRSTKPTTWRRIAEDASLRRSLLLRCEALRAVREWFWARGFV
                     EIDAPVLAPWVGMEPHLVPLETRMSDGKRALRVFHQTSPEYALKKLLTAGIPDCFALG
                     HVFRDGEVSPMHNPEFTLLEWYRRGADYTALMDDTACLTAELAVKLCGAPIIQVGGKQ
                     IDLTPPWERITVAEAMQRWAGVDIEANVEDEAFRAAAREAGNDWVTNEPWEDLFFKLF
                     LTHVEPRLGWPKPVILCEYPVRFAALARRSLENSLVAERFEAYIAGVELCNGYSELTD
                     PVEQRERLESEQRQRAALGAEVLPVDEDFLDALGGGFPVCAGNALGVDRLVMLMAGET
                     DIARVTWFPFSVMAEWAERHGR"
BASE COUNT          442 a          901 c          817 g          479 t
ORIGIN      
        1 accgggcaca cccgcacgcg gtggggcagg cattcctgcc tgccatatgc ttctcttcac
       61 ttccccttct tctcgaccgc cacgtgctcg ctgctcacca cgcatccgcg ttcgtcaacc
      121 agatttatgt agtgccccgt cgcgccggcg ggcaccttcg ccgaggcctt cccggccttc
      181 gcatccagcg atgccggcac cgtctcccag ttgcgcgtca tccagttgcc cgtgtccgtc
      241 gtccagttga actccgcagc caccaccggc acctcgctct cgaactccac cgatgaccgg
      301 ccgcagcgga cgccctgctt cgtgatccgg gccagcgcag gctttccacg gaacagatgc
      361 tccgccatgg cgagtatctc cggcggcgcc tccccggccg ggccgtgggc gtgttccata
      421 cggacgcgaa ccgccagcgt gcgcccatcc ttcggcaggc ggtacgagag ctgcaatgcg
      481 tccatcggat acgcgaaatc gttcgtgccg gtcacccaga ccatcggcat ggtggcgccc
      541 ggcagataca ccgacggatc ccacattcgc agccacttct cggccttttc cttccccatg
      601 ttcgcaaacg tttccagcca cgcggagttt aggcccagga acccgcagcc gtacaccggc
      661 gccgcgaact tgaaccgctg atccacgccg gcgacgatgc acgtcgtgta gccgccccat
      721 gagaccccgg tcagcccggt gcgctcggcg tccacctcgg ggaacgaccg gatgagtgaa
      781 tgcccgagaa ttgccgcggc gacggcgtgg tacgtccact gatcctccac cggctcgtcc
      841 agcgcgtcga agccgcccca gcccgccggg ccgccgtgct cgtggcgagg gtgggggtcg
      901 ccatagtcgc cgtcgttcgt gcagccgcag caatccatgg agatggcggc atatccccga
      961 tccagccaaa gcttcaccca cgggatgaag gccgagccgc ccccgccgtg gatcagcacc
     1021 atggcgggca ccttctcgcc ctttttcacc ttcgggatgg cgtaatacgc gaacaccttc
     1081 gtcggtttac ccctgaacgg cacgccctcg tagaacacgg ctttcatgtc cttggtgctg
     1141 cgcgtcggat cgagctccca cgcgggcgtg gcggagaggc gtgtcatatc ccagggggtg
     1201 gaccaccgct cagcggcagg tgtggacgcg gtggacggct tcgcgttggc ggtgggtttc
     1261 tttgttgctg gcattagggg gctccatctg gcagggtacg tcagagcaag aactggacat
     1321 cgaaaacagt atggaagatc acttgttgaa caaatcccgc ccccctcacc tttcccccta
     1381 gcccccctcg ctttcgatgt tttaaagacc acgcaccgca cacaaaaggg tccaccgtgt
     1441 ccacccggtc cacaaaaccg accacctggc gtcgcatcgc agaggacgcc tccctgcgcc
     1501 ggtcgctgct cctccggtgc gaggcgctgc gtgccgttcg cgagtggttc tgggcgcgcg
     1561 ggttcgtgga gatagacgca cccgtgctgg cgccgtgggt cggcatggag ccgcatctcg
     1621 tcccgctgga aacgcggatg tccgacggca agagagccct tcgtgtgttc caccagacct
     1681 cgcccgaata cgcactgaag aagcttctca ccgccggcat acccgattgc ttcgcgctgg
     1741 ggcatgtgtt ccgcgacggg gaggtttcgc cgatgcacaa cccggagttc acccttctcg
     1801 agtggtaccg ccgcggcgcg gactacaccg ccctgatgga cgacacggct tgcttgacgg
     1861 ctgagctggc cgtcaaactt tgcggcgcgc cgatcatcca agtcggcggc aaacagatag
     1921 acctcacccc accgtgggaa cgcatcaccg tcgccgaagc gatgcagcgc tgggcggggg
     1981 tcgacattga ggccaacgtg gaggacgagg cgttcagggc ggcagcgcgg gaggcaggta
     2041 acgactgggt taccaacgaa ccatgggaag atctgttttt taagctgttt cttacccacg
     2101 tcgagccgcg cctcgggtgg ccgaaaccgg tgatcctgtg tgaataccct gttcggttcg
     2161 cagcccttgc gcggcggagc ctggaaaact ccctcgtcgc cgagcggttc gaggcctaca
     2221 tcgccggggt ggagctctgc aacgggtaca gcgaactcac cgacccggtg gagcagcgcg
     2281 agcggctgga gtccgagcag cgccagcgcg ccgcgctcgg tgcggaggtg ctgcccgtgg
     2341 acgaggactt tctcgacgcg cttggtggtg ggttccccgt ctgtgcagga aacgcgttag
     2401 gggtggacag gctcgtgatg ctgatggcgg gagagaccga catcgcccgg gtgacgtggt
     2461 tccccttctc cgtcatggcc gagtgggcgg agcggcacgg gcgttgaacc gctccttggg
     2521 ccgtttcccg agtgccgcgt tggttcccgt tcccgtccgc attaccctcg gatagacaaa
     2581 cccacccgtc ccgacaaccc gaagaaaggt cgttccatga gcgaacttca cgttgacac
//
LOCUS       DAOSOO010000330         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_40676_length_2548_cov_5.570397, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000330 DAOSOO010000000
VERSION     DAOSOO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40676_length_2548_cov_5.570397"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /gene="dsbD"
                     /locus_tag="PLT86_14510"
     CDS             <1..1602
                     /gene="dsbD"
                     /locus_tag="PLT86_14510"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HOT37871.1"
                     /translation="GKPVPKFKGRIEFRTPLFLARTANPVRLTIPVEVRAQACSEEMC
                     FFPSQQVISAAITVAPRTEEALQMPRDVGSARLDSAREAALTAHAPDSASRPGSSEGF
                     NLGSLLAERGFAFAFGLIFLGGVLTSFTPCVYPLIPITVSFFASRGGAGGKKSRLRGL
                     LLSVVFVLGMATMYSLLGVLAAATGSVFGAVMANPVVIGAVALLFVAFAFSMFGAFEI
                     TLPVSLQTRLSTIGGAGGLTGVFAAGLVAGIVAAPCTGPVLGAVLTYVATTGNTPFGF
                     VALFVFALGMGLLFVAIGTFSSSLMPKPGPWLEKIKSVFGIVMLVMALYFLGGVFPPL
                     QTLWLSGLRGVAISLVLTFLGLLTGAVHLRFHAGFDREGNPEAPPTIWERSRKAAGII
                     LCVAGIYGVVLTATSPPAKEAATANGVTWDSDEASALARAIEQRKPILIDAYAEWCVA
                     CRELDAITFADPRVQQRLRSFVTLKLDFTAITPETQKLRQKYGIVGLPTVLVLDENGV
                     EEKEKRLVGFEPPDKFLKRLDRSYP"
     gene            complement(1624..1758)
                     /gene="rpmH"
                     /locus_tag="PLT86_14515"
     CDS             complement(1624..1758)
                     /gene="rpmH"
                     /locus_tag="PLT86_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT37872.1"
                     /translation="MKPTFQPHVLRRKRTHGFRARMATPGGRKVLSRRRAKGRLRLCP
                     "
     gene            complement(1853..>2548)
                     /gene="panB"
                     /locus_tag="PLT86_14520"
     CDS             complement(1853..>2548)
                     /gene="panB"
                     /locus_tag="PLT86_14520"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT37873.1"
                     /translation="FVEAAGVDMALVGDSLGNAVYGFRTTRDVTMELMLPHVAAVRRG
                     APNTHIVADMPFQSFDTPELAVANARRFVDVGADSVKIEGALTDVVEALRDAGIEVMG
                     HVGLLPQTAKDFKARGRTQEEADAICAGARALERAGCFSLVLEHMPLGLAQRITEELS
                     VPTIGIGAGPHCDGQVLVIHDMLGFFFPGGRFKPPFVKRYAAIGEIAVEACRRYVEEV
                     RSGAFPDLAHSTV"
BASE COUNT          482 a          772 c          743 g          551 t
ORIGIN      
        1 gggaaaccag ttcccaaatt caaaggccgt atagagtttc gtaccccgct gttcttggct
       61 cggaccgcga atcccgtaag gctgaccatc cctgttgagg tgcgcgcgca ggcatgttcc
      121 gaggaaatgt gcttcttccc gtcgcaacag gtaatttcgg cagcaattac ggtggccccg
      181 agaacggaag aggcgctcca gatgcctcgc gatgtcgggt cagcgcggtt ggattccgcg
      241 cgagaagccg ctcttacggc gcatgcgcca gattcagcat ctcgccccgg ttcgagcgaa
      301 gggttcaacc tcggttcctt gttggctgaa cgagggttcg ccttcgcgtt cggactgatc
      361 tttctgggcg gggttctcac cagtttcacg ccgtgtgtgt atccactgat ccctatcacc
      421 gtgagtttct ttgcgtcgag aggaggtgcg ggagggaaaa agtcgcgcct tcggggtctc
      481 ctgttgtctg ttgtgttcgt tctcggcatg gcgacgatgt acagcctcct cggtgttctt
      541 gctgctgcga cagggtccgt cttcggtgcc gtgatggcga atcctgtcgt gatcggggcg
      601 gtcgcgttgt tgtttgttgc gtttgcgttc tccatgttcg gcgcgtttga gatcacgctg
      661 cctgtatcat tgcagacaag gctctccacg attggtggcg cgggcgggct caccggcgtc
      721 ttcgcagcgg gtctcgtcgc cggaatcgtt gcggcaccat gcaccggtcc ggttctcggc
      781 gcagtcctga catacgtcgc aacaaccggc aataccccgt tcggattcgt ggcgttattc
      841 gtgtttgcgc tcgggatggg attgctcttc gtagcaatcg gcaccttttc atccagcctc
      901 atgcccaagc ccggaccgtg gctcgagaaa atcaaaagcg tgttcggaat cgttatgctc
      961 gtgatggcac tctatttcct tggaggggtt ttcccaccgc ttcaaacgct ttggttgagc
     1021 ggtctccgcg gcgttgcaat ttcactggtt ctcacgtttc tcggtctcct gacaggagca
     1081 gtccaccttc ggttccacgc cggatttgac cgcgaaggga accccgaggc ccccccgacg
     1141 atctgggaac gatctcggaa ggctgcaggc ataatactct gtgtcgcagg catatacgga
     1201 gttgttctga cggccacctc ccctcccgcg aaggaagccg cgacggccaa cggcgtaacg
     1261 tgggattctg acgaagcctc ggccttagcc cgcgcgatag agcaacggaa accaatcctg
     1321 atagatgcgt acgccgagtg gtgcgtggca tgccgggaac tggacgcgat cacgtttgcc
     1381 gatcctcgag tgcaacagcg cttgcgttcg ttcgttaccc tgaagctgga tttcacggca
     1441 ataaccccgg aaacacagaa acttcggcag aagtatggta tcgtcggctt gccgacggtt
     1501 ctggtgctgg acgagaacgg cgtggaggaa aaagaaaagc ggctcgtggg cttcgagccg
     1561 cctgataagt tcctgaaacg actggaccga agctatccgt gacgtgcccc ggcctccagt
     1621 gacctacggg cacaaccgga ggcggccctt ggcacgacgg cgactcagaa ccttgcgccc
     1681 gccgggcgtc gccatgcgcg cacgaaagcc gtgggtccgc ttcctgcgca gaacgtgcgg
     1741 ttggaatgtt ggcttcatcg gtatccctca ttcatgtcaa tggacagcgc ggaaaaacaa
     1801 acaagtctat taatatacgg attcttgatt ttcggtcaac catggtgacg ctctatactg
     1861 tgctgtgagc aagatcgggg aaggctccag atcgcacttc ttccacgtag cgcctgcagg
     1921 cctccacggc gatttcacca atggcggcgt accgcttgac aaaaggaggt ttgaagcggc
     1981 cgcccggaaa gaaaaacccc agcatgtcgt ggatgacgag cacctgcccg tcacagtgag
     2041 gtcccgcccc tatcccgata gtcggtaccg aaagctcttc ggtgattctc tgcgccaggc
     2101 ccagaggcat atgctccaac accaggctga aacatcccgc tcgttccaaa gcccgggcgc
     2161 cagcacagat ggcgtccgcc tcctcctgag tgcgccctcg cgccttgaag tccttcgccg
     2221 tctgaggaag caggccgacg tggcccatca cctctatccc cgcatcgcgg agcgcctcga
     2281 ccacgtccgt cagagcgcct tcaattttca ccgagtcagc accgacgtcc acaaagcgac
     2341 gcgcgttcgc cacggcgagt tcaggagtgt cgaagctctg gaacggcatg tccgccacaa
     2401 tatgagtatt gggtgctccc cgccgcaccg cggctacgtg aggcaacatc agttccatcg
     2461 tcacgtcgcg cgtggtgcgg aaaccgtaca ctgcgtttcc cagggaatcc cccaccagcg
     2521 ccatatccac ccctgcagcc tcgacgaa
//
LOCUS       DAOSOO010000331         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_40861_length_2539_cov_4.366345, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000331 DAOSOO010000000
VERSION     DAOSOO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40861_length_2539_cov_4.366345"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLT86_14525"
     CDS             <1..308
                     /locus_tag="PLT86_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37874.1"
                     /translation="TCTPRVFSPGTTQGYSDKTVIAFTVSTADAGNSATISIFNTAGR
                     LVREMAPSIVAGANSIAWNGTDDGGSIAPSGMYIVAVKVGGVEKRQTVVILNKYARP"
     gene            374..>2539
                     /locus_tag="PLT86_14530"
     CDS             374..>2539
                     /locus_tag="PLT86_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37875.1"
                     /translation="MRMHSAHTRRLRQGTTGIACSIGIMCMVAAAHAGTPSTTVGVGA
                     RPLGMGGAFVGLADDAHAIYWNPAGLPRLQRQEFTGSYMPNRLISGLNTGYGAATIPI
                     GERQAVGADMFYESYSDNELSFTSFNLRASYGLSLFNWLSVGGNAKYVAPLSVKYNNN
                     QVDDPRGFGFDVGTLLDFGNLLPALEGLRLGAVVRDVGGTSVKHDNDFSENIYPATYV
                     VGASYKLNDSFTFAADLDDRARFGVEYSLLNLLSLRAGIQRTVQGYGTGMYYNGGAGI
                     KWRGIKLDYAYETHPTLNPTHYMTLSFVYNPSFVTIRDARINRTPLFRALYRSYEQEP
                     EFAQVTLKNTSQDPLRVNVGLRIPTLMAEGSSHAQDFTLPPQSTQTVSLGVTLDDSLL
                     IRDISNYDNLVQPEVFVNYTQERETKQAQKKLSSVFVLGKNKLTWLNPTVAAAFVTPD
                     HTPIVSFADRAASSFRSVRDTEFSTCPNYGTAMILFAAVSKYGVLYNPDQNTPFYKIA
                     SDTSQLGNIFDTVKFPIETLRSRLGDCDDVSVLFISLLESQSVPTALLDVFDPALGHI
                     YMMFDTGLTPEQAMQSGLFLSEDEFVTWTDPGQEVPEAHAWIPVETTMFGQPFSEAVR
                     VGLAEYREKKARNYIREWSIAQGRSQYQAGILDSASVAFPNVGDVQQYLAAEIERMKR
                     RLELPPLEEPVTAEKLYMRGAELRQRGQYAQAIEAFSEAI"
BASE COUNT          520 a          755 c          773 g          491 t
ORIGIN      
        1 ttacctgcac gccgcgcgtg ttttctcccg gcacgacgca agggtacagc gacaagacag
       61 tgattgcgtt taccgtgtca accgcggacg cgggcaattc cgcgacgatc agcatcttca
      121 acaccgcggg gcggctggtg cgggagatgg cgccctccat tgttgccggc gccaattcca
      181 tcgcgtggaa cggcacggat gacggcggca gcatcgcacc cagcgggatg tacatcgtcg
      241 ccgtgaaggt cggaggagtc gaaaagcgtc agacagtggt gattctgaac aagtacgcgc
      301 gtccatgacc acgatacatt gagcccgcgt caaaccggtt caggggttgt catacgacat
      361 aaggtgaggc gttatgcgga tgcactcagc gcacacacga aggcttcgtc aggggacgac
      421 tggcattgcc tgttcaatcg gcatcatgtg catggttgcg gcggcacatg cgggcacccc
      481 cagcacaacg gtgggagtgg gggcccgacc tctgggaatg ggtggtgcct tcgtcggcct
      541 cgcggatgac gcgcacgcca tctactggaa tcccgccggt ctaccgcggc ttcagcggca
      601 ggaattcacc ggcagctaca tgcccaaccg cctcatcagt ggcctcaaca ccggttacgg
      661 ggccgccacg atccccatcg gggaacggca ggcggtcggc gcggacatgt tctacgaatc
      721 gtacagcgac aacgagctgt cgtttacgtc gttcaatctg cgcgcgtcgt acggtttgtc
      781 gctgttcaac tggctgtcgg tgggcgggaa cgcgaaatac gtcgcgcccc tttccgtgaa
      841 atacaacaac aaccaggtgg acgatccgcg cggattcggc ttcgatgtcg gcacgctgct
      901 ggatttcggg aacctcctcc cggcgctgga agggctgcgg ctcggagcag tcgtgcggga
      961 cgtcggggga accagcgtca agcacgacaa cgacttctcg gagaacatct accccgccac
     1021 gtacgtggtc ggagcgtcgt acaaactgaa cgacagcttc acctttgccg cggatctgga
     1081 tgaccgcgcg cggtttggcg tcgaatacag cctgctcaac ctcctttccc tccgggcggg
     1141 cattcagcgg acagtccaag ggtatggcac cggcatgtac tacaacggtg gcgcgggtat
     1201 caagtggcgg gggatcaagc tcgattacgc gtatgagacc cacccgacgc tgaacccgac
     1261 gcactacatg acgctgtcat tcgtctacaa cccgtcgttc gtgacgatcc gggatgcccg
     1321 gatcaaccgg acgcctctct tccgggcgct gtaccggtcg tacgaacagg aacccgagtt
     1381 cgcgcaggtt acgctcaaga acacctcgca ggacccgctc cgcgtgaacg tcgggttgcg
     1441 gataccgacc cttatggcgg aaggttccag ccacgcgcag gatttcacgc tgcctcccca
     1501 gagcacacaa acggtatcgc tgggcgttac gctggatgac agcctgctca tccgcgacat
     1561 ctccaactac gataacctcg tgcagccgga ggtgttcgtg aactacaccc aggaacggga
     1621 gacgaagcag gcgcagaaga agctttccag cgtgttcgtt cttggtaaga acaaacttac
     1681 ctggctcaac ccgaccgtgg ccgcagcgtt tgtaacacca gaccatacac cgatcgtgag
     1741 tttcgccgac cgggcagcca gcagtttccg ttccgtgcgt gatacggagt tttccacatg
     1801 cccgaattac ggaacagcca tgatcctctt cgccgcggtc agcaagtatg gcgttctgta
     1861 caacccggac cagaatactc ctttttacaa aatcgcgagc gacacctccc agcttggtaa
     1921 catcttcgat acggtcaagt tcccgatcga aacgctgcgt tcgcgccttg gcgactgtga
     1981 tgacgtgagc gtgcttttca tctcgcttct ggaatcgcag agtgttccaa ccgcgttgct
     2041 ggacgtgttc gaccctgcgc tgggacacat ttacatgatg tttgacaccg gtctcacgcc
     2101 cgagcaggcg atgcagagcg gtctgttcct ctcggaggac gagttcgtta cgtggacgga
     2161 ccccggtcag gaagtgccgg aggctcatgc gtggattccg gtggagacga cgatgttcgg
     2221 ccagcctttt tccgaggccg ttcgagtcgg gcttgccgag tatcgggaga aaaaggcgcg
     2281 gaactacatc cgagagtggt ctattgcgca ggggcggtct cagtatcagg caggaatact
     2341 tgactcggca agcgtggcat ttccgaacgt gggtgacgtt cagcagtacc tcgccgcaga
     2401 gattgaacgg atgaagcgcc gtctcgagtt gccgccgctg gaagaaccgg tcactgccga
     2461 gaagctctac atgcggggcg cggagcttcg ccagcgaggg cagtatgcgc aggccataga
     2521 agccttcagc gaagcgatt
//
LOCUS       DAOSOO010000332         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_40979_length_2534_cov_6.637354, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000332 DAOSOO010000000
VERSION     DAOSOO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40979_length_2534_cov_6.637354"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLT86_14535"
     CDS             <1..184
                     /locus_tag="PLT86_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37876.1"
                     /translation="QSNDRDAIEAMTRRMCEVGRMCRGYFMCIGNHIPWNVPAEAVKW
                     YFDCSERYGVLDASTL"
     gene            complement(278..1657)
                     /gene="ltrA"
                     /locus_tag="PLT86_14540"
     CDS             complement(278..1657)
                     /gene="ltrA"
                     /locus_tag="PLT86_14540"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOT37877.1"
                     /translation="MEQKTRQLAFDWGEDAELRPGPSEGLDTCPPPSCTGALAHGLME
                     AVVAADNMRQALKRVRANKGSPGVDGMSVDELKGYLMGAWPQIREALLEGTYRPQPVK
                     RVDIPKPGGGTRQLGVPTALDRLIQQALLQVLSPLYEPTFSPHSYGFRPGRSAHDAVD
                     AARGYVAERKMWVVDLDLEKFFDRVNHDVLMGLVRQRIGDRRVLRLIRRYLQAGVLFN
                     GVVTDRYEGTPQGGPLSPLLANILLNELDKELERRGHCFCRYADDCNIYVGSKRAGLR
                     VMSSVTEFLERRLRLRVNRDKSAVALPWHRKFLGYRIIMQREVRLTIAPESLQRAKHK
                     IRRITRRNRGVSLERVLQELRTFTDGWVTYFRHARSPSLFEDLDQWIRRRLRCYVWKQ
                     WKKPRKRARELMNAGVAPDVAWRTAYSGARLWRMAGSRAVTQALSNRRLADLGYHSLL
                     ERYEALAVA"
     gene            complement(2135..2434)
                     /locus_tag="PLT86_14545"
     CDS             complement(2135..2434)
                     /locus_tag="PLT86_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37878.1"
                     /translation="MAEPYVHRSFEGEAIMTVGKAVDFARKGLSGVVAVMPFTCMPGT
                     ISEALMKRVREDENEIPFLNMVYDGFEQSTARTRLEAFMYQAREYMMRNRTAAVS"
BASE COUNT          430 a          866 c          750 g          488 t
ORIGIN      
        1 gcagagcaat gaccgggacg caatagaggc gatgacgcgg cggatgtgcg aggtcgggcg
       61 catgtgccgg gggtacttca tgtgcatcgg caatcacatt ccgtggaacg tgcctgcgga
      121 agcggtgaag tggtacttcg actgttcgga gcgctacggg gttctggacg cctcgacgct
      181 ctgaacgaaa cccgcgacca accgaacatc gggtaggcgg ggtcgttgcc ggccccgccc
      241 cccacaccac ccggcatgcg ggtccgcacc gggcggttca cgcaactgca agcgcctcgt
      301 accgttcaag taagctgtgg tagcccaggt ccgcaagtcg tctgttgctc agtgcttggg
      361 tcacggcgcg gctgcccgcc atacgccaca acctcgcccc actataggcc gtcctccagg
      421 ccacgtccgg ggccacgccc gcattcatca gctcccgcgc acgtttccga ggtttcttcc
      481 actgcttcca cacgtagcac cgcagccgtc gcctgatcca ctgatccagg tcttcgaaca
      541 gcgagggact gcgcgcatgg cggaaatacg tcacccaacc gtcggtgaag gtccgcagct
      601 cttgcagcac tcgctccagg ctcactcccc gattgcggcg cgtgatgcgg cgtatcttgt
      661 gcttcgcgcg ctgcaaactt tccggggcga tggtcaggcg cacttcgcgt tgcatgatga
      721 tccgatagcc caggaacttg cgatgccacg ggagggccac cgcgctcttg tcgcggttca
      781 ctcgcaatcg cagcctgcgt tccaggaatt ccgtcaccga actcatcacg cgaagtcctg
      841 cgcgtttgct gccgacgtag atgttgcagt cgtcggcgta tcgacagaag caatggcctc
      901 ggcgctccag ttccttatcc aactcgttca gcaggatgtt ggcaagcagc ggagacagcg
      961 gaccgccttg aggagtgcct tcgtaccggt ctgtcaccac gccgttgaac aggacaccgg
     1021 cctgcaggta ccggcggatc aggcgcagca cgcgccggtc gccgatgcgc tgccggacaa
     1081 ggcccatcaa cacatcgtgg ttcacccggt cgaagaactt ctcaaggtcg agatccacca
     1141 cccacatctt cctctccgcc acgtatccgc gggccgcgtc caccgcatcg tgggcgcttc
     1201 tgcccggtcg gaagccgtag ctatggggcg agaaagtggg ctcgtacaac ggggacagca
     1261 cttgcagcag cgcctgctgg atcagccggt ccaacgccgt cgggacgccc agctgtcgcg
     1321 tcccgccacc cggcttcgga atgtccaccc gcttaaccgg ctgcgggcga tacgtccctt
     1381 ccagtagggc ctcgcggatc tgcggccacg cgcccatcag atagcccttc agctcatcga
     1441 cactcatccc gtcgacgccg gggctgccct tgttcgcgcg gacgcgcttc agagcttgcc
     1501 gcatgttgtc ggcggcaacc acagcctcca tcaggccgtg cgccaaggct cccgtgcatg
     1561 aaggcggcgg acacgtgtcg agcccttcac tggggcccgg acgtaattca gcgtcctccc
     1621 cccagtcgaa ggccagttgc ctggttttct gctccatggc gtgcccgatc cttcacgtgg
     1681 gtccttgtag ctggtgttca gcccttccct catgctgcga ggtactatgg cctttgctga
     1741 cttctgcccg cccatccccg accgttgccg atcgagtagc cacagctgtg gcagacgagc
     1801 agacctcccg ggataagctc atgatctttc gccccgcgcc tgccggatct acaccgggcc
     1861 gcctttggag acctcgggtt tcactgtcgt ccgccagctc acccggcgcc cgatgccttc
     1921 tatccggttt ctgttcgtca ggtcgtggct gtggctaagg cttctttcag accccgcctc
     1981 gcggcgacgc ccttgccttc gcttaacggt tatggtctcc gataccgtag gggacttgca
     2041 cccccgagat catcagcatg cccggcatac agacgaaggc ggcgcacctc aacacaggtg
     2101 cgccgctctt gcctctgcga aaagcgtggc cacgtcagga cacagccgcc gtgcggttgc
     2161 gcatcatgta ctcccgcgcc tggtacatga aggcctcaag acgcgtgcgg gcggtagact
     2221 gctcgaagcc gtcgtagacc atgttcagga acgggatctc gttctcgtct tcgcggacgc
     2281 gcttcatcag ggcttccgag atggtgcccg gcatgcaggt gaagggcatc accgcaacca
     2341 ccccgctgag gccctttcgc gcgaagtcca cggccttgcc cacggtcatg atcgcctcac
     2401 cctcgaaact gcggtgcacg tagggctcgg ccattgtcag gacttcttcg gtaggcggct
     2461 cgtaggcatt gcgcaacagc ccgtcgaagg cggcgatcag ttggtgctag tcggagacca
     2521 tcacccgatc catg
//
LOCUS       DAOSOO010000333         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_41848_length_2494_cov_4.387864, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000333 DAOSOO010000000
VERSION     DAOSOO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41848_length_2494_cov_4.387864"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLT86_14550"
     CDS             complement(<1..725)
                     /locus_tag="PLT86_14550"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOT37879.1"
                     /translation="MSDKRVTRWLAFLMFLVVFGIYFRTMAPTTSFWDCGEFIACSFT
                     LGIPHPPGSPLFIMLGRVFSMFPIEGFLKAWGWLPEYADGAARVNIMSPLAGALSALF
                     CYLVILRLIRGWQGPLGTVKERWSDHVGAVAGTLIFALADSNWFNAVEGEVYAYAIFL
                     MMLALYLGLVWADSIGKPIHLPLALFLAYLMGLASGLHLLCLLVLPTIAFIGLFTYVK
                     DRYDAWKIVGAAALLVIGAYFAM"
     gene            1039..1728
                     /gene="cysE"
                     /locus_tag="PLT86_14555"
     CDS             1039..1728
                     /gene="cysE"
                     /locus_tag="PLT86_14555"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOT37880.1"
                     /translation="MFRDVKAVFERDPAARGFWGVFEVVFTYAGLHALWNYRVAHFLH
                     RHGVPFIPRLLSQIGRFLTGIEIHPGAQIGRGFFIDHGMGVVIGETAIVGDDCTLYQG
                     VTLGGTGKETGKRHPTLGNGVVVGAGAKVLGNITIGNNVYIGANAVVLKDVPDDSTVV
                     GVPGRVVRQRGARVASALEHTGIGDPIMLKLDALQRDMARFEQELRAIQNRDRDGVSE
                     NPEPEEPRPAQ"
     gene            2038..>2494
                     /locus_tag="PLT86_14560"
     CDS             2038..>2494
                     /locus_tag="PLT86_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37881.1"
                     /translation="MGRHPNILQITTHDSGRHFGCYGHPTIHTPAIDALAAEGVRLTN
                     YFAAVPICSASRATQLTGLYPQTHGLMDLCGYPFRWRIRDDVRHMSQLLKAAGYTTHL
                     FGIQHEVIPDELPRLAFDSIRGQGFPSAKVIAADVAGFLRSRRREPAPFY"
BASE COUNT          519 a          796 c          745 g          434 t
ORIGIN      
        1 tgcatcgcga agtacgcgcc gataaccagc agggccgcgg cgccgacgat cttccacgcg
       61 tcataccggt ccttgacata cgtgaaaagg ccgatgaacg cgatggtggg gagcacaagc
      121 agacacaaca ggtggagccc gctggccagt cccatgaggt acgccaggaa cagggcaagc
      181 ggcaggtgaa tcggtttccc aatcgaatcg gcccacacca ggccgagata cagcgccagc
      241 atcatgagga agatggcgta cgcgtacacc tcgccctcaa ccgcgttgaa ccagttggaa
      301 tccgccagcg cgaaaatcag tgtgcctgcc accgcgccga catggtccga ccagcgttcc
      361 ttcacggttc cgagagggcc ctgccagccg cggatcagac gcagaatgac gaggtaacag
      421 aacaacgccg aaagagcgcc ggctaacggg gacatgatgt tcactcgcgc ggcaccgtcg
      481 gcgtattccg gaagccatcc ccacgccttc aggaacccct cgatcgggaa catcgagaac
      541 acgcggccca gcatgatgaa cagcggggac cccggtggat gagggattcc cagcgtgaac
      601 gagcatgcga tgaactcccc gcaatcccag aaggatgtcg tcggtgccat ggtccggaaa
      661 tagatcccga acacgacaag gaacatcagg aacgcgagcc aacgggttac ccgtttgtca
      721 ctcatgccac aatccctctg gttgaatcac gcggaaatcg tgatgccgcc cgcccgaaat
      781 cggaacttcc tcgccgcctc ggggggtaat ggtggtagag attagagcag attgcatccg
      841 ctcaaagact cgttgagaaa tgtgagaata ccgcccgtgc gcggccaagt caagcgttgc
      901 acccggcgga gccacgccgt ggcccgcgag agcgatccgg ccgattgacg tcgccctcac
      961 gcgccgagta ttcttctagt ccgactgcag acgatgcgcc agggcgcccg gcgcgccggg
     1021 caaaaggagc cagaacgtat gtttcgcgac gtcaaggcgg tttttgaacg cgatcccgca
     1081 gcccgtggct tctggggcgt cttcgaggtg gttttcacct acgccgggct ccacgctctg
     1141 tggaactatc gcgtggcgca ttttctccac aggcatggcg tcccgttcat tccccggttg
     1201 ctctcccaga tcgggcgctt tttgaccggc atcgagatcc atccgggcgc acagattggc
     1261 cgcgggttct tcattgacca cggaatgggt gtcgttattg gcgagacggc gattgtcggg
     1321 gacgattgca cgctgtatca aggcgttacg ctggggggaa cgggaaaaga aaccggcaag
     1381 cgccacccga cgctgggaaa tggcgttgtg gtgggcgcgg gcgcgaaggt tctcggcaac
     1441 atcacgattg gcaacaacgt ctacatcggt gccaacgccg tcgtgctcaa agatgtccct
     1501 gatgacagca ccgtggtggg cgttcccggc cgcgtggtga ggcagcgcgg cgcacgcgtc
     1561 gcttccgcgc ttgagcacac cggaatcggc gaccctatca tgctgaagct cgacgcgctc
     1621 cagcgcgaca tggctcgctt cgaacaggag ttgcgcgcca tccagaatcg cgaccgcgac
     1681 ggtgtttcgg agaacccgga accggaagaa ccccgtccgg cgcaataatg tcggccaccg
     1741 cggccacacc tccttccgcc gtccaatatg gcggaaggaa ggtacgagtc ggcaaccata
     1801 gtctccctgt ctcaagttcc tatccccatt atcctcaact tgttgtcgca ccccagagcc
     1861 cgcagcccgc gtaccccgat tgaacttttt tcacagaaac acttgacacg aacaccccgc
     1921 gtcgccacag cttgatcgag gtcacccggc ggtagggtac cggcggaagg gcgtacgcgg
     1981 gggttgggct gggcatccgt aatcgccatt ttgcacagca gggaaaagga gcaagccatg
     2041 ggcagacacc ccaacattct gcagatcacc acgcacgatt ccgggcgcca tttcggctgc
     2101 tacgggcatc ccacgattca cacgcccgcc atcgatgcgc tggctgccga gggtgttcgc
     2161 ctcaccaact acttcgccgc cgtgccgatc tgctccgcca gccgcgccac gcagcttacc
     2221 ggtctctacc cgcagacgca cggcctcatg gacctctgcg ggtacccctt ccgctggcgc
     2281 atccgagatg acgtgcgtca catgagccag ttgctcaagg cggcaggcta caccactcac
     2341 ctgttcggca tccagcatga ggtcatcccg gacgagttac cccgtctggc gttcgactcc
     2401 atccgggggc agggtttccc gtccgcaaag gtgatcgccg ctgatgtagc gggtttcctg
     2461 cgttcaagga gaagggagcc agctccattc tatg
//
LOCUS       DAOSOO010000334         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_42218_length_2478_cov_4.904251, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000334 DAOSOO010000000
VERSION     DAOSOO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42218_length_2478_cov_4.904251"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2478
                     /locus_tag="PLT86_14565"
     CDS             <1..>2478
                     /locus_tag="PLT86_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37882.1"
                     /translation="GPVVSVAFMGDWGGITGSLDMTAKIWGGEGGSSGPTNVSGEIMS
                     QTWTRQGSPYRIVGPVMVPPSETLNIDSGVEVQFVTNVPFVVAGGLNINGSPMDSVWF
                     HGFDSNWGGIRFVGMEPVAKPSYGPAPSAYLNGVIIEGGSASGETTAEQSGGGIYVGP
                     GREVNISNSSIRGNAAGYAGGGIYVDGGSLWLYQSKVSGNSAGAMGGGIAAGGGSVNL
                     SQSGVAGNTAQTRGGGIALEEAASLYLWQANVANNNALVEGGGISLLGGSSANLENAQ
                     IVRNYTNGSGGGIFGRNSSMNSWRMVVAQNTGRNSGGGIAGEYMTVNMNFATVADNVS
                     PTGAAAFALANSETNVLNSIIWGHATGNPLSFGEGGYTNITYSDVQVPWGGTGNISAD
                     PMFVDPANLAYALQPGSPCIDAGAPESTDEDGTRADMGAFPTWQEGMQKKLVLPNVNA
                     APGGEVLVDIKATHPGLVSTDLFFTIPSNVFTPAENFIRWHVFEGTPAAQPEVVWTMT
                     GDTVKISIATNTDYPAHLNDSPLITLAFNVNPEAQYGWYGLGWLQSVWKPETQEWMYT
                     NLNEEQVYMEWGGIELQQSILWGDVTMDETVSGADASAILRHVVGLPNDTFDREVADV
                     TGNGWVSSFDAALILRKVLFPWFHFPVEGPQKLAASGATSIANLSWERDGSGWVLRSN
                     TAGIEAGELTLTLPGDAPVTISGDNALAYRQDGTTVQIALVNAAASGILFRVDGVTSA
                     PMISTASFNEGAMTLAPMLPTAFALDQNAPNPFNPTTTIRFALPEASQVNLVVYDLNG
                     RAIRTLATGAFQPGNHSVVWDGLDANGR"
BASE COUNT          475 a          733 c          799 g          471 t
ORIGIN      
        1 cggccccgtc gtgagtgttg cgttcatggg cgactggggc ggcatcacgg gcagcctcga
       61 catgaccgcc aagatttggg gcggcgaggg tggttcatcc ggcccgacga acgtctcagg
      121 tgagatcatg tcacaaacgt ggacacggca gggcagcccg taccgcatcg tcggcccggt
      181 gatggttccc ccttcggaga cactcaacat cgactccggc gtcgaagtgc agttcgtcac
      241 caacgttccc ttcgtcgtcg ccggtggttt gaacattaac ggctcgccga tggacagcgt
      301 gtggttccac gggtttgaca gcaactgggg cggcatccgg ttcgtcggga tggaaccggt
      361 tgcgaaaccc tcgtacggac cagcgccgtc ggcgtacctc aacggcgtca tcattgaggg
      421 tggctcggcc tccggcgaaa ccaccgcgga acagagcggc ggcggaatct acgtcggtcc
      481 cggccgagag gtgaacatct ccaattcgtc tatccgcgga aacgccgccg ggtacgcggg
      541 cggcggcatc tacgttgatg gtggatcgct ctggttgtat cagtccaaag tgagcgggaa
      601 cagcgcagga gccatgggcg gaggcatcgc cgcaggcggt ggttccgtta acctctctca
      661 atcaggcgtc gccggtaaca ctgcgcaaac tcgcggcggt ggcatcgccc tggaagaggc
      721 agccagcctg tatctctggc aggcaaacgt cgccaacaac aacgcactgg tggaaggcgg
      781 gggtatttcg ctcctcggcg ggtcgagcgc caatctggaa aacgcgcaga tcgtccggaa
      841 ctacacgaac ggttccggcg gtggcatttt cggccggaat tcctcgatga actcctggcg
      901 catggtagta gcgcagaata ccggcaggaa ttcgggcggt ggcatcgctg gtgaatacat
      961 gaccgtcaac atgaacttcg ccaccgtggc ggacaatgtt tcaccaaccg gcgcggcagc
     1021 gttcgcattg gcaaacagcg aaacgaacgt tctgaactct atcatctggg ggcacgcgac
     1081 aggcaatccg ctctcttttg gcgagggtgg ctacacgaat atcacctact cggatgttca
     1141 ggttccatgg ggaggtacgg gcaacatctc tgccgacccg atgttcgtcg atcctgcgaa
     1201 tctcgcatac gcccttcagc cgggttctcc gtgcatcgac gcgggtgccc ccgagtctac
     1261 ggatgaggac ggcacacggg ctgacatggg tgcgttcccc acgtggcagg aagggatgca
     1321 gaagaagctg gtgctcccga acgtgaacgc ggcgccgggt ggcgaggttc tggtggacat
     1381 caaggcaaca cacccgggtc tggtgtcaac cgatctgttc ttcaccatcc cctccaacgt
     1441 gttcaccccg gcggagaact tcatccgctg gcacgtgttc gagggaaccc cggctgccca
     1501 gcccgaagtg gtctggacca tgaccggcga cacggtgaag atcagcattg ccacgaatac
     1561 cgactatccg gctcacctga acgacagccc gctcatcacg ctggcgttca acgtgaaccc
     1621 ggaagcacaa tacggctggt atggccttgg ctggctgcag tctgtctgga agccggagac
     1681 acaagagtgg atgtatacga acctgaacga agagcaggtc tacatggagt ggggcggcat
     1741 cgagttgcag cagtccattc tgtggggtga cgtaacgatg gacgagacgg tatcgggcgc
     1801 cgatgcctcg gccatcctgc ggcacgtggt gggcctgccc aacgatacgt tcgatcgtga
     1861 ggtagcggac gtaacgggca acggctgggt gtcgtccttt gacgcggcgc tcatcctccg
     1921 caaggttctc ttcccgtggt tccacttccc ggtggaaggc ccgcagaaac ttgcggcgtc
     1981 gggagccacc tccatcgcga acctttcgtg ggagcgtgac ggttctggct gggtgctgcg
     2041 ttcgaacact gccggcatcg aagcgggtga actgacgttg accctgccgg gtgacgcgcc
     2101 tgtcacgatc tctggcgaca atgcgcttgc gtatcgtcag gacggcacga cggtgcagat
     2161 cgctctggta aatgcagcgg cgagcggcat cctgttccgt gtcgacgggg taacctccgc
     2221 cccgatgatt tccactgcgt cgttcaacga aggcgcgatg acgctggcac ccatgcttcc
     2281 cacggcgttc gcgcttgacc agaacgcgcc gaacccgttc aacccgacga ccacgattcg
     2341 gtttgcgttg ccggaggctt ctcaggtgaa tctggtggtg tatgacctga atggccgtgc
     2401 aattcggaca ctggcaacgg gcgcgttcca gccgggtaat cactcggtgg tgtgggacgg
     2461 tctggatgcc aacggccg
//
LOCUS       DAOSOO010000335         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_42852_length_2452_cov_7.753442, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000335 DAOSOO010000000
VERSION     DAOSOO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42852_length_2452_cov_7.753442"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1791)
                     /locus_tag="PLT86_14570"
     CDS             complement(190..1791)
                     /locus_tag="PLT86_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOT37883.1"
                     /translation="MDNFAILTVIVVVYLFVVAYLGYYGYRKTKNATDYLVAGRSTHP
                     YIMALSYGATFISTSAIVGFGGAAALFGMGLLWLTFLNIFFGIFIAFVVFGKRTRKIG
                     RNLDAHTFPEFLGRRFDSRFIQGFAGITIFLLMPLYASAVLIGAARFIENTFAGVNYN
                     TAILIYTLIICAYVLAGGLKGVMYTDALQGTIMFVGMLVLAVYTYSTLGGVIDSHQAL
                     TNMADKVPAKLAAIGHQGWTSMPALGSPLWWQLVSTIVLGVGIGVLAQPQLVVRFMTV
                     KSSRELNRAVLSGGIFILAMTGVAFVVGALSNVFFYEHTAYKAISLAVAKGNIDSIIP
                     LYVNSAMPVWFVYLFMITLLSAAMSTSSSQFHTMGSAFGRDFFEKTLFRGKQFLYTVS
                     LTRIGIVLGVIATVILGYNLPGSVIAIATAIFFGFCATMFLPSFVGALFWKGMTRAGA
                     ISSVVVGFFGSACWLLFVHQKESEALGLCKLVFGKASLAAGTVWAMVDPIVVILPISF
                     VVAFGVSMWTKKMPQEHLDACFKHM"
     gene            complement(1867..2037)
                     /locus_tag="PLT86_14575"
     CDS             complement(1867..2037)
                     /locus_tag="PLT86_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37884.1"
                     /translation="MLGIADPQIWLAYVLCILSTILCVYYGAKNWDKGDEPVYPEDVT
                     WAEDEKKATAEN"
BASE COUNT          559 a          737 c          728 g          428 t
ORIGIN      
        1 cgtcttccgg gactcccttc ggtcgcgggc ggaagggacg ttgtttcacg gtcaaaaccg
       61 tgaggaaggt tctccctccg ggggaacgaa acgaagccag accggtaacg cagtggagta
      121 gccgcagtaa agaaaagagg gacgcagatt gccccgcgtc cctctttcct ttcctcttgc
      181 ggccacatgc tacatgtgtt tgaagcatgc gtcgaggtgc tcctgcggca tcttcttcgt
      241 ccacatgctt acaccgaacg caacgacgaa ggaaatcggc agaatcacca cgatcgggtc
      301 caccatcgcc cagacggtcc cggcggcgag gcttgccttg ccgaacacga gtttgcacag
      361 cccgagcgcc tctgattcct tctgatgaac aaacagaagc cagcacgcgg aaccgaagaa
      421 ccctaccacc acgctggaaa tcgcccccgc ccgcgtcatg cctttccaga acagcgcgcc
      481 cacgaagctc ggaaggaaca tggtggcgca gaacccgaag aatatcgcgg tcgcaatggc
      541 gatgacactt ccgggaaggt tgtagccgag aatcacggtg gcgatcacgc cgaggacaat
      601 cccgatacgc gtcaggctga cggtgtacag gaactgcttc ccgcggaaca gggtcttttc
      661 gaagaaatcc cgcccgaacg ccgatcccat ggtgtggaac tgcgaactgg aggtggacat
      721 ggccgcggaa agcagagtaa tcatgaacag gtacacgaac cagaccggca tcgcgctgtt
      781 gacgtacagg ggaatgatgg aatcaatgtt ccccttggca accgccagtg agatggcctt
      841 gtacgcggtg tgctcgtaga agaacacgtt ggagagagcg ccaaccacga aggcaactcc
      901 cgtcatcgcc aggatgaaga ttccgccgct caataccgcg cggttgagct cgcggctgct
      961 cttcaccgtc atgaagcgca cgacgagttg cggctgcgcc agcacgccga tgccaactcc
     1021 cagcacgatc gtcgagacaa gctgccacca gagcggcgaa ccgagcgcag gcatgctggt
     1081 ccacccctga tgcccgatcg cggccagttt cgcgggaacc ttgtccgcca tgttggtaag
     1141 cgcctggtgg gagtctatca ctccgcccag cgtggaatac gtgtagaccg caagcaccag
     1201 catcccgacg aacatgatcg tgccctgcag ggcgtcggta tacatgacgc ctttcaggcc
     1261 gcccgccagc acgtacgcgc agatgatgag cgtgtagatc aggatggccg tgttgtagtt
     1321 gacccccgcg aacgtgtttt caatgaagcg cgcggccccg atcagcaccg cggaggcgta
     1381 caacggcatg agcaggaaaa tggtgatgcc ggcgaacccc tggatgaacc gggaatcaaa
     1441 ccggcgcccc aggaattcgg gaaacgtgtg ggcgtcgaga ttgcgcccga ttttccgggt
     1501 tctcttcccg aacaccacaa aggcgatgaa gatgccgaaa aagatgttca ggaacgtgag
     1561 ccacaacagc cccattccga acaacgcagc cgccccgccg aatcccacaa tcgcggacgt
     1621 actgatgaag gtggcgccgt acgacagcgc catgatgtac gggtgtgtgc tccttcccgc
     1681 aacaaggtaa tctgttgcgt ttttcgtctt ccggtagccg taatatccca gatacgcgac
     1741 aacaaacagg tagacgacta cgatgacggt aaggattgcg aaattgtcca tctggtatcc
     1801 cccgaggaat caatggtgtt tcgatcgtct gcgttcaatc gtgaaccacg tggtgccaac
     1861 acggagtcag ttctccgcgg tcgccttttt ctcgtcctcc gcccaggtga cgtcctcggg
     1921 gtacacaggt tcgtcgccct tatcccagtt ttttgcgccg tagtagacgc acaggatcgt
     1981 gctgagaatg caaagaacgt aggccaacca gatctgcggg tccgcaattc caagcatacc
     2041 gttctccctt cgtgtgcggc gggccgcccc ggcaacctgc cggcggcaac ccgtctgttg
     2101 acggacgcag cgcaatggcg cgacaacccg gagcggaaac cgtaggcatg tgcacgcgcg
     2161 tgcagtcagc agggcgactt tcgcgcatgc actcgcggct gccgtacgaa aaacctttcg
     2221 cgagtggacg cgtcatgcgt atgaaagatt gaacgcggcg aaagaatacg caggaatgtg
     2281 cgcccgcgca aaccgggcgg gtcccctgaa gctccacgag gcgctcagaa gaacggccgg
     2341 ttgcgtacgc ggcgggagac agggaccgcg tttttctgtt gccggcacgg gcgatgcacc
     2401 atcacagacg gggtggaaat gccgcacgcg aagccgcgaa ggacacgaag ag
//
LOCUS       DAOSOO010000336         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_42918_length_2449_cov_4.681287, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000336 DAOSOO010000000
VERSION     DAOSOO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42918_length_2449_cov_4.681287"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="zwf"
                     /locus_tag="PLT86_14580"
     CDS             <1..1129
                     /gene="zwf"
                     /locus_tag="PLT86_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT37885.1"
                     /translation="LATAPEFYALAVTMLGTTGMAAETEKTGYRRVIVEKPFGHDYAS
                     SVALNATLHDVFAEQQVYRIDHYLGKETVQNILVFRFGNAIFEPLWNRRYVDHVQITV
                     AETVGVGHRAAFYETAGVIRDMFQNHLLQLLTLIAMEPPYTYEADALRNEKVKVLQAI
                     RPIDEERFLRDVVLGQYSGYREEKGVSPRSETPTYAAVRLLIDNWRWQGVPFYLRSGK
                     KLAQKASEISVVFRRPPHQLFGDHCEFFTNRLTLCIQPNEGLHLRFSAKVPDAGMVMQ
                     AANMGFSYEKWFAERVIPEAYERLLLDALNGDPSLFARSDEIEYAWKIVDSVHSAWEK
                     NPEYHVQPYEPGTWGPKAADQMLTRTGGHWTHGCGALDEVE"
     gene            complement(1422..2060)
                     /locus_tag="PLT86_14585"
     CDS             complement(1422..2060)
                     /locus_tag="PLT86_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT37886.1"
                     /translation="MESETLPQRYGRNSGAKMRRGAYLPHVTADNAMYAVTFRLADSV
                     PRSLLDKWRAEREDILRSAQQAGRPLSAFGTRRLRDVFSREVDQHLDRGAGACWLKDD
                     SIAAVAALALWHFDGDKYLLLAWCVMPNHVHAVVRPEKGHELPDIVHSWKSFSAKRAN
                     EMLRRRGRFWHTEYYDHLIRNDRDLEHSVQYVLQNPEKAGLKNWRWTGCADV"
     gene            complement(2158..>2449)
                     /locus_tag="PLT86_14590"
     CDS             complement(2158..>2449)
                     /locus_tag="PLT86_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37887.1"
                     /translation="DGHCSYSPDLRWILYDSYVDSSTPDFLRNLQVYSLDRREGILLG
                     RFRAGPLTPDNNDLRCDLHPRWMPGGTAVTFDSIHEGYRGLYWADLSGVVGR"
BASE COUNT          486 a          809 c          714 g          440 t
ORIGIN      
        1 cctcgccacc gcgcccgagt tctacgcgct cgccgtcacc atgctcggca ccaccggcat
       61 ggcggcggaa accgagaaaa ccggctaccg gcgcgtgatc gtggagaaac cgttcgggca
      121 cgactacgcc tcctcagtcg ccctcaacgc aactctgcac gacgtgtttg ccgaacaaca
      181 ggtctaccgc atcgaccatt acctcggcaa ggaaacggtg cagaacatcc tcgtctttcg
      241 gttcgggaac gccattttcg aacccctctg gaaccgccgg tacgtggatc acgtacagat
      301 tacggttgcg gagaccgttg gcgtgggcca ccgggctgcg ttttatgaga ccgccggcgt
      361 gatccgcgac atgtttcaga accacctcct gcaattgctg accctgatag ccatggagcc
      421 gccgtacacc tacgaggcgg acgcgctccg gaacgagaag gtgaaggtcc tgcaggcaat
      481 ccgcccgata gacgaggaac ggttcctgcg cgatgtcgtg ctggggcaat acagcgggta
      541 ccgcgaagaa aagggtgtct cgccgcgttc cgaaacacct acgtatgccg ccgtgcgcct
      601 gctcatcgat aactggcgct ggcagggcgt gccgttctac ctgcgttcgg gcaagaagct
      661 ggcgcagaag gcgtcagaga tcagtgtcgt gttccgtcgt cccccgcatc agctgttcgg
      721 cgatcactgc gaattcttca cgaaccgcct caccctctgc atccagccaa acgaaggcct
      781 ccacctgcgc ttttccgcaa aagtgcctga tgccggaatg gtgatgcagg cggcgaacat
      841 gggcttcagc tacgagaagt ggttcgccga gcgcgtcatt cccgaggcgt acgagcgcct
      901 ccttctcgac gccctcaatg gcgacccctc cctgttcgcc cgcagcgacg aaatcgagta
      961 cgcgtggaaa atcgtggatt ccgtccactc cgcgtgggag aagaacccgg aataccacgt
     1021 ccagccgtac gaacccggca cctggggccc caaagccgcc gaccagatgc ttacccgtac
     1081 cggcggccac tggacgcatg gttgcggcgc gttggacgaa gttgagtaga gccgcacccg
     1141 gtcattgagc ctgcgcggtc cctgagcttg tcgaagggcg cgcaggagcc tgtcgaacca
     1201 tcgaaggtgc cggggcacca gagtgcgaac gtgcttgcca ccgccggtgc ccgtattggc
     1261 agcactggac ccggagtaac ggcccaggtc ccggcacggg cgagacgccc gtgctacgcg
     1321 ttgcggttca tacgtcacaa agacagccca cgatctgccc gggaagcaga gctcccgtag
     1381 cacgggcatc ttgcccgtgc ctgagccggg ctgcaacctg ctcacacgtc agcacacccc
     1441 gtccatcgcc agtttttcag ccccgccttc tccgggttct gaagtacgta ctggacggaa
     1501 tgctccaaat cccggtcgtt ccgaatcaga tggtcgtagt actccgtgtg ccagaaacgc
     1561 cctctccggc gaagcatctc gttcgcgcgt ttcgccgaaa acgatttcca tgagtggacg
     1621 atgtccggca actcgtgccc tttctcgggc cgaaccacag catgaacgtg gttcggcatc
     1681 acacaccagg ccagcaacag gtacttgtct ccatcgaaat gccagagcgc cagcgcagca
     1741 accgcagcga tggaatcgtc tttcaaccag caagcccccg caccgcggtc gaggtgttgg
     1801 tccacttccc gggagaatac gtctcgtaat cgtctcgttc caaacgcgga caacggccgg
     1861 cccgcttgtt gcgcggaccg caaaatatct tcccgttcgg ctctccactt gtcgagcaga
     1921 gatctgggca ctgagtccgc gaggcggaaa gtgactgcat acatcgcgtt atcggccgtc
     1981 acgtggggca ggtacgctcc ccgccgcatt tttgccccgg agttgcggcc ataccgttga
     2041 ggaagagtct ctgattccat tcccggaaat tccacctcaa tctggcacgg gcgagacgcc
     2101 cgtgctacga atcccgagcg ggacggacgt tccgcccgtg cacgaattgc gtctctccta
     2161 ccttcccacc accccgctca aatccgccca gtacaatccc cgatacccct cgtggatgga
     2221 atcgaacgtg accgccgtgc caccgggcat ccagcggggg tgaaggtcgc agcggaggtc
     2281 gttattgtca ggggtaagtg gccccgcgcg gaaccggccc agcaggatcc cctcgcgccg
     2341 gtcgagcgaa tacacctgca ggttgcgcag aaaatccggc gtcgagctgt ccacgtagct
     2401 gtcgtacaga atccagcgca gatcgggcga ataggagcag tggccgtcg
//
LOCUS       DAOSOO010000337         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_42982_length_2446_cov_3.925136, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000337 DAOSOO010000000
VERSION     DAOSOO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42982_length_2446_cov_3.925136"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLT86_14595"
     CDS             <1..362
                     /locus_tag="PLT86_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37888.1"
                     /translation="RHRKSEAEHMREDAIATAREMKKRITVETLSRELPNVHAMPRRG
                     TEIEIDSALVRKAADAYDSVYQAKRAIADELKMDLGALPGWMVEEIEAALSEGLGRDR
                     VRLIAEYVGGFVQRQAV"
     gene            416..1447
                     /locus_tag="PLT86_14600"
     CDS             416..1447
                     /locus_tag="PLT86_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT37889.1"
                     /translation="MTPKPFWSHWGLTRNPFGGISGADDVFESREVERIMERLYEAVE
                     EGGIHAVTGERGIGKTTAKNEVLNHFDENRSRFAYSIVECMDLRIVTMSTINTAIVTD
                     LSSERPKASNEARSRQVTRILGELSPHKKVVLVIDEAQRLPIETLEKLKMLTERRWGV
                     RTKLITVLLFGQAELSYKLARDEGLMLRVTQYRMAGLTSDEVLQYIDLRCRVAGGNMR
                     DIFEEDVLVYVAENQHSPLHINHVCGSAMRMARNAGEKKVTLAMIYECGGIRNPRQIL
                     KDNNVTVKRFAEIVHMHDQQVTKLLDGNMEGVSPDQRERFRNGLSNLTRGTQHDTEYH
                     GGSGERKSA"
     gene            1428..2075
                     /locus_tag="PLT86_14605"
     CDS             1428..2075
                     /locus_tag="PLT86_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37890.1"
                     /translation="MSARARDDTDAGQIHAYLMLRLGHKWRFETWRKKYEARLRVYGA
                     EKCRIAVDGFCSGTWWVANHGHNAPDLIFRSDRSFERFLAMGLKLPRHSEEEREKRAT
                     AAAQRDRRTEYRRELEAKNAELTRRFHAKIAGLKDELQENTWEMFIAPLLYVGYEDGV
                     VVLFHETAGWVQDCYSGKIERELGKKVKIVSEPMKDYPPVLRTSPLDEGGHKKKY"
     gene            2104..2319
                     /locus_tag="PLT86_14610"
     CDS             2104..2319
                     /locus_tag="PLT86_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37891.1"
                     /translation="MKPIRISGHARRRTRQRHRDFTASEMVAMAALARTCGMVGPGYN
                     GCRRYKFLGMEFVFDEKAKEPVLVTVI"
BASE COUNT          593 a          621 c          880 g          352 t
ORIGIN      
        1 accgccatcg gaagagcgaa gccgaacaca tgcgggaaga tgccatcgcg acggcgcgtg
       61 agatgaagaa gcgcatcacg gtggagacgc tttcgcgcga actgccgaac gtccacgcca
      121 tgccccggag agggacggag atcgagatcg acagcgcgct tgtccggaag gcggcggatg
      181 catacgacag cgtataccag gcgaagcggg caatcgcgga cgagttgaag atggacctgg
      241 gcgcactgcc gggatggatg gtggaggaga tcgaggcggc gctttccgaa ggactgggac
      301 gcgaccgggt gcggctgatc gcggagtatg tgggcggatt cgtacaacgg caggctgtgt
      361 aagagtgttt ctcgcaaagg gcgcgaagag cgcgaaggaa aggacggaaa agaggatgac
      421 accgaagcca ttctggagcc actggggact gacgcggaac ccgttcggag gcatctccgg
      481 ggcggacgat gtgttcgaga gccgcgaggt agagcgtatc atggaacgcc tgtacgaggc
      541 ggtcgaggaa ggcgggattc acgcggtgac cggcgaacgc ggcatcggga agacgaccgc
      601 gaagaacgaa gtgctgaacc actttgacga gaaccggagc cggttcgcgt acagcatcgt
      661 ggagtgcatg gacctgcgaa tcgtgacgat gagcacgatc aacacggcga tcgtcacgga
      721 cctctcaagc gagcggccga aggcgagtaa cgaggcgagg agccgtcagg tgacgcgcat
      781 cctcggggag ctttcacccc acaaaaaggt ggtgctcgtg atcgatgagg cgcagagatt
      841 gcccatcgag acgctcgaaa agctgaagat gctcacggaa cggcgctggg gtgtgcggac
      901 aaagctcatc acggtgctcc tcttcgggca ggcggaactg tcgtacaagc tggcgcggga
      961 cgaggggctg atgctgcggg tgacgcagta ccgtatggcc gggctgacat cggacgaggt
     1021 gctccagtat atcgacctcc ggtgccgggt cgcgggcggg aacatgcggg acatattcga
     1081 ggaggatgtg ctcgtgtacg tcgcggagaa ccagcactcc cccctccata tcaaccatgt
     1141 gtgcgggagc gccatgcgga tggcgaggaa cgccggagaa aagaaggtca cactcgccat
     1201 gatctacgaa tgcgggggga tacggaaccc ccggcagatc ctcaaggaca acaatgtgac
     1261 ggtgaagcgg ttcgcggaga tcgtccacat gcacgaccag caggtgacga aactcctcga
     1321 cggcaacatg gagggggtat cgcccgatca gcgtgaacgg ttcaggaacg gactttcgaa
     1381 cctcaccagg ggaacgcagc acgacaccga atatcacggg ggaagcggtg agcgcaagag
     1441 cgcgtgacga caccgacgcc gggcagatcc acgcgtacct aatgttgcgg ctcgggcaca
     1501 agtggcggtt cgagacctgg cggaaaaagt acgaggccag gctccgggta tacggcgcgg
     1561 agaagtgccg tatcgcggtg gacgggttct gctcggggac gtggtgggtc gcgaaccacg
     1621 ggcacaacgc gccggacctc atcttccgga gcgaccggag cttcgagcgg tttctcgcga
     1681 tggggctgaa gctcccccgg cactcggagg aggagcggga aaaacgggcg acggcggcgg
     1741 cacagcggga ccgcaggacg gaataccgca gggaactgga ggcgaagaac gcggagctga
     1801 cgcggcgttt tcacgcgaag atcgcggggt taaaggacga gctgcaggag aacacgtggg
     1861 agatgttcat cgcccccctg ctctacgtgg gatatgaaga cggtgtggtc gtcctcttcc
     1921 acgagaccgc gggctgggtg caggattgct acagcgggaa gatagagcgg gaactgggga
     1981 agaaagtgaa aatcgtgtct gaaccgatga aagattatcc ccctgtcctt cggacatccc
     2041 ccttggacga agggggacac aagaaaaagt actgaacctc gattcgtggg aaagggaccg
     2101 gccatgaaac cgatacggat cagcggacac gcgagaaggc gcacccggca gagacatcgg
     2161 gactttaccg cctcggagat ggtcgcgatg gcggcgctcg cccggacgtg cggcatggtc
     2221 ggccccggat acaacgggtg caggaggtac aagttcctcg gtatggagtt cgtattcgat
     2281 gagaaagcga aggagcctgt gctggtgacg gtgatttgaa gacaggcaca gaggcacaaa
     2341 ggcagaaggc acagagggaa aagcaaagaa agtctgaacc tcggatggcc gggatgacgc
     2401 ggataacaga ggatgaaagc gggaaactga tggaaaactc gaagat
//
LOCUS       DAOSOO010000338         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_43274_length_2432_cov_5.811527, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000338 DAOSOO010000000
VERSION     DAOSOO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43274_length_2432_cov_5.811527"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLT86_14615"
     CDS             <1..391
                     /locus_tag="PLT86_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37892.1"
                     /translation="APITVDGRTYSWVTARPDSTGRVSLDRVFGIVDLSLAYLSTAIY
                     SPVDQRVPIQIGVDNFFQLFLNGQMVAGGEAYGMPYERKILPLDLKAGWNSLYLKVVN
                     NRGNWGVEASVIDLRGNLRFAPYPGQN"
     gene            454..2268
                     /gene="aspS"
                     /locus_tag="PLT86_14620"
     CDS             454..2268
                     /gene="aspS"
                     /locus_tag="PLT86_14620"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT37893.1"
                     /translation="MGGSGTDRLSGWRRTHRCGDLRASDIGLDVILMGWASSHRDHGG
                     VIFVDLRDRWGITQVVFDPAVSKEVHKRAEHIRQEYVIAVKGRVRARMEGMANAKLAT
                     GEIEVICNELRVLNSAETPPIQVDGPPANEEIRLKYRYLDLRRPIMQRNLELRHAAAQ
                     ATRRFLCAQDFVEIETPTFVSSTPEGARDYLVPSRVWPGRFYALPQSPQIYKQLLMVA
                     GMDRYFQIVRCYRDEDLRADRQPEFTQIDVEMSFVEEADVMQVAEGITRSVYKETIGY
                     DVPDSFPHLDYADAMSRYGSDKPDIRFGLEMVDVTDIAAESDFKVFRAVTEAGGIVSA
                     INVKGGASFSRKQVDDMTPFVAQYGAKGAAWMKVAEQGLESNIAKFFNEGVQNALRER
                     LSAEQGDLLVFVADQAPIVYQALGNLRLKFGNDLNLIDKSKFAFLWVTNFPLLSWDEE
                     ENRWVANHHPFTSPVLEDLDLFVSGGDPGRIRARAYDLVINGTEVGGGSIRIHQRHVQ
                     QQMFDLLGISPEEAERRFGYLLNAFRYGAPPHGGIAFGFDRMVMQLAGQESIREVIAF
                     PKTNSAASPMDGSPSEVDERQLRELGLRLRHVKHEGTA"
BASE COUNT          498 a          638 c          812 g          484 t
ORIGIN      
        1 agctcccatc acggtggacg gcaggacgta ttcgtgggtt accgcacggc cggattcgac
       61 cggacgcgta agcctggaca gagtgttcgg tatcgtggat ctgtcgctgg cgtatttgtc
      121 aacagctatc tacagccccg ttgatcaacg ggtgcctatc cagatcggtg tggacaattt
      181 cttccagctc tttctcaacg gccagatggt tgcgggcggg gaggcgtacg ggatgccgta
      241 tgaacggaaa atcctgcccc tcgacctgaa agcgggctgg aactcactct acctgaaggt
      301 tgtgaataat cgcggcaact ggggcgttga agcatccgtg atcgacctgc ggggcaatct
      361 gcgttttgca ccctatccgg ggcagaactg agacaggggt ttggggcgtt attccggcgt
      421 attgggacga tagacacaaa ggtgagggac acaatgggtg ggagtggaac agaccggctt
      481 tccggttgga ggcgtacgca caggtgcggg gatttaaggg cttctgacat aggattggac
      541 gtcatcttga tggggtgggc gagttcccat cgagaccatg gcggcgtcat cttcgtggac
      601 ctgcgcgacc gttggggtat cacccaggtt gtcttcgatc cggcggtgag caaggaagtg
      661 cacaaacggg cggaacacat ccgccaggaa tacgtgattg ccgtaaaggg gcgcgttcgt
      721 gcacgcatgg agggaatggc gaacgcgaaa cttgcgaccg gggaaatcga ggtcatctgc
      781 aacgaattgc gggttctcaa tagtgcggaa accccgccaa tacaggtgga cggccccccc
      841 gcgaacgagg aaattcgcct gaagtaccgg tatctggacc ttcggcgccc catcatgcag
      901 aggaaccttg agttgcgcca cgccgcggcg caggccacac gcaggttcct gtgcgcgcag
      961 gatttcgtgg agatcgaaac gcccacgttc gtgagcagca cgccggaagg agcgcgggat
     1021 tatcttgttc cgagccgcgt gtggccgggc aggttttacg cgcttcccca gtctccacaa
     1081 atctacaagc agcttttgat ggttgccggg atggatcggt atttccagat tgtccggtgt
     1141 taccgcgatg aggacctgcg cgcggatcgc cagccggaat tcacccagat cgacgtggaa
     1201 atgtcgttcg tggaagaggc ggacgtcatg caggtggccg agggcatcac gaggtcggtg
     1261 tacaaagaaa cgatcgggta tgacgttccg gattcgtttc cccatctgga ttatgccgat
     1321 gccatgtcgc gttacgggtc ggataagccg gacatccgtt ttggccttga gatggttgat
     1381 gttaccgaca tcgcggcgga gtctgatttc aaggtgttcc gggcggttac ggaagcgggc
     1441 ggcattgtca gtgcgatcaa cgtgaaaggc ggggcgtcgt tcagccggaa acaggtcgac
     1501 gacatgacgc cgtttgttgc acagtacggg gcaaaaggcg cggcctggat gaaggttgcc
     1561 gagcaggggc tggaatccaa tatcgcgaag ttcttcaatg aaggcgtgca gaacgcgctc
     1621 agggaacggc tcagcgccga acagggtgac ctgctggtgt tcgttgcgga ccaggcgccg
     1681 atagtgtatc aggcgctggg caacctgcgc ttgaagttcg gcaatgacct caacctgatc
     1741 gacaaatcga agtttgcgtt cctgtgggtg acaaacttcc ctctcctctc gtgggacgag
     1801 gaagaaaacc ggtgggtggc aaaccatcac ccgttcacgt cccccgttct ggaggatctg
     1861 gaccttttcg tgtcgggtgg cgatcccggg cgaatccgtg cccgggcgta cgacctcgtg
     1921 atcaacggca ccgaagtggg tggcggaagc atccgcatcc accagcggca cgtgcagcag
     1981 cagatgtttg accttctggg catctccccc gaggaagccg aaaggcgctt cgggtacctg
     2041 ctcaacgcgt ttcggtacgg ggcgcctccg cacggtggta tcgccttcgg gtttgaccgc
     2101 atggtgatgc agcttgccgg gcaggagtcc atcagggagg tgatcgcgtt tccgaagacc
     2161 aacagtgcgg cgtctccgat ggatgggagt ccttcagaag tggacgagcg gcaactgcgc
     2221 gaactgggtc tcaggcttcg gcacgtgaag catgaaggaa ctgcataacg ccgcgttgcg
     2281 aagtcgtacg ccacgggagg cgcggccaca atggtcgcgc ctcccgtttt gtatcggatc
     2341 cgatacgcgg aggaggggag cacccggggg aaagacgggc aggcaccaaa gcggcgtccg
     2401 cgcgtacaga gattcgtgta cgattgcggc ac
//
LOCUS       DAOSOO010000339         2428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_43365_length_2428_cov_5.469027, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000339 DAOSOO010000000
VERSION     DAOSOO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43365_length_2428_cov_5.469027"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLT86_14625"
     CDS             complement(<1..675)
                     /locus_tag="PLT86_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT37894.1"
                     /translation="MRLSIVEAARECPTRTALYAEGTRYTFGELSARTQQRMREFTAV
                     LPKGVAGSSVAVIASNDLETTVSLFALWELEIPALLIHPRAPLAERTRLLEVFQPAAS
                     VRGREVHPSGPFARDIPEGTACLILTSGTTGHPKAAVLSHAAFVASAEASATRLQWRS
                     DDRWLASLSIAHVGGLSIITRCLLARSCVVLHRRFSEDKFVRALEEERVTQVSLVPTM
                     LERVLER"
     gene            complement(656..1810)
                     /locus_tag="PLT86_14630"
     CDS             complement(656..1810)
                     /locus_tag="PLT86_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOT37895.1"
                     /translation="MTPQIREIGIYPDARVGPRRGTLAVLRDRHGMTGIGEASPLVGH
                     SPETLEAANSVLERFDLALLPPPIPAPRWEATLLTSFQCVAEESPAAAMALETAWIDL
                     AAQQADLPFAAFLSGEMASGARVPVNVLVNSLDQESSLHRIKRALSAGITTVKLKATD
                     IHNVQVTLDMIASIRREWGDAVSIRVDANGAWPLDLAMPVLKKLADWGVEYCEQPVPR
                     GELRMLSPSPVPLAADESLTDMKELEAFLGIPAKRINEFLRGLVTPEDVLKAAPEGFS
                     PPVKVFVLKPMVLGGILRSRRIARIAAERGIRYTVTHLFDGATAFAAYAEFAVSLQPP
                     PLACGLFPHDATAAAGTVDDSHFDGSCVRSAHQPGLGVNRGAILHAALDR"
     gene            complement(1807..>2428)
                     /gene="menA"
                     /locus_tag="PLT86_14635"
     CDS             complement(1807..>2428)
                     /gene="menA"
                     /locus_tag="PLT86_14635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOT37896.1"
                     /translation="RAATVAVFGSACVLGSLLTLHSGWPVAAIGIASIVAALAYTAGP
                     YPLGYHGLGDVFVMLFFGFVAVCGTAFVQMQTVPGSAWITAVAVGALSTAILVVNNVR
                     DSENDRTTGKRTLAVILGLRLAKGEYLALLVTAYLVPVAVFVFFVPHAWVFLPLASTP
                     LAILTARRFLSATTGAEYNSVLAQTGVLLLTYSVLLSTGLVLPALR"
BASE COUNT          504 a          748 c          754 g          422 t
ORIGIN      
        1 acgctccagc acccgttcca gcatcgttgg tacgagggac acctgagtca ctctttcttc
       61 ctccaacgca cgcacgaact tgtcttcgct gaagcgtcgg tgcaaaacga cacaggaccg
      121 cgcgaggagg caacgcgtga taatggaaag gcctccgacg tgtgcaatcg agagcgaggc
      181 cagccagcgg tcgtctgacc tccactgaag acgggtggcg ctggcctcgg cacttgcgac
      241 gaacgccgcg tgggagagca ccgccgcttt tgggtgcccg gtcgtgccag aggtcaggat
      301 gaggcacgcg gtcccttccg ggatgtcccg tgcgaatggt cccgaagggt gcacctcgcg
      361 ccccctcacg gacgctgccg gttggaacac ctccagaagg cgggttctct cggcaagggg
      421 tgcgcgcggg tggatgagga gagcggggat ttccagttcc cagagcgcaa aaagggacac
      481 cgtcgtttcg agatcattcg aggcgatcac cgccacactg ctgccggcga cgcctttcgg
      541 caggaccgcg gtgaactctc gcatgcgttg ttgcgttcgt gcgctgagtt cgccaaacgt
      601 gtaccgcgtt ccctctgcgt acagtgccgt acgagtcggg cactcccgcg ctgcttcaac
      661 gatcgagagc cgcatggaga attgctccac ggttgacgcc gagcccaggt tgatgggcgc
      721 tccggacgca agacccgtcg aaatgactgt cgtccaccgt tcccgccgcg gcggttgcgt
      781 cgtgcggaaa caggccgcac gcaaggggcg gcggctgaag cgaaaccgcg aactcggcgt
      841 acgcggcgaa cgcggtcgct ccgtcaaaaa ggtgcgttac tgtgtagcgg attccgcgtt
      901 ctgccgcgat gcgcgcgatc cttcgcgatc gcagaatgcc gccaagcacc atcggtttga
      961 ggacgaacac tttcaccggt ggcgaaaagc cttcaggcgc ggctttcagg acatcttcgg
     1021 gtgtcactaa gcctcggaga aactcattga tgcgtttcgc cgggatgccc aggaatgcct
     1081 caagttcctt catgtcggtg agcgactcat cggccgcgag aggcacgggg gatggactca
     1141 gcatccgtaa ctcgcccctt ggaacgggtt gctcgcagta ctcgactccc cagtcagcga
     1201 gctttttgag gaccggcatc gccaagtcga gcggccacgc gccattggcg tccactctga
     1261 tggaaactgc gtccccccac tcgcgccgaa tggaagcgat catatccagc gtaacctgca
     1321 cattgtgaat gtctgttgcc ttgagtttga cagtcgtgat cccggctgac aacgcgcgtt
     1381 tgattcggtg caggctggat tcctggtcga gcgaattcac gaggacgttc accggaacac
     1441 gagcacccga cgccatctcc cctgaaagaa aggcggcgaa gggcaggtca gcctgctgag
     1501 ccgccagatc gatccaggcg gtttccaacg ccatcgcggc ggcgggggat tcctctgcca
     1561 cgcactggaa cgaggtcagg agcgtggctt cccaccgagg cgcggggatc ggcggaggca
     1621 gaagggccag gtcgaatctt tcaagcaccg agttcgctgc ttccagggtc tctggcgagt
     1681 gccccacaag cggcgacgcc tctccgattc ccgtcattcc gtgtctgtct ctcagcaccg
     1741 cgagagtgcc gcggcgcggt cccacacgag cgtcgggata gatcccgatt tcgcgaattt
     1801 gcggtgtcat ctcaaggccg ggagaacaag tcccgtggaa agaaggacgg aatacgtcag
     1861 gagcaaaaca cccgtttgtg caagaacaga gttgtactcc gctcccgttg tcgcagaaag
     1921 gaagcgtcgc gccgtaagta ttgccagggg cgtcgatgcg agcggcagaa acacccacgc
     1981 gtgcggcacg aaaaacacga aaacggcgac gggaaccagg tacgctgtga caagtaatgc
     2041 gaggtattcg cccttcgcaa ggcgaagacc gagaataact gccagcgttc gcttccctgt
     2101 ggtccggtcg ttttcagaat cgcgcacatt gttcacgaca aggattgcgg tgctcaacgc
     2161 tccaacggcg accgccgtga tccacgcgga cccgggaacg gtttgcatct gaacgaacgc
     2221 cgttccgcac accgcaacaa acccgaagaa caacatcacg aaaacatcgc cgagaccgtg
     2281 gtaccctaac ggatagggac cggcggtgta tgccagtgcg gccacaatcg aggcaatgcc
     2341 tatcgccgcc accggccagc ctgaatggag tgtgagcagc gatccaagaa cacacgcgga
     2401 cccgaacacg gcaaccgtcg ccgcgcgc
//
LOCUS       DAOSOO010000340         2417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_43636_length_2417_cov_5.944539, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000340 DAOSOO010000000
VERSION     DAOSOO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43636_length_2417_cov_5.944539"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLT86_14640"
     CDS             <1..598
                     /locus_tag="PLT86_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT37897.1"
                     /translation="MGYTRRAVALKRIAERVIRAYDGRLPRSPEHLQTFPGIGAATAG
                     AICAYAYNLPTVFIETNIRRVFIHTFFGEHETVSDKQILPLVEETLDHSQPRVWYYAL
                     TDYGAALGRRLENPNRKSAGYRRQGRFEGSKRQIRSTIVRELLARGALTLGDIDQLCS
                     GTPFEGGSILRELQRDGLVQPLGDQWCVAFTPDSAGTV"
     gene            complement(599..1633)
                     /locus_tag="PLT86_14645"
     CDS             complement(599..1633)
                     /locus_tag="PLT86_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37898.1"
                     /translation="MSYERGIQAIRLQMPDTIPHTEYVSNPTLVKHVTGIDPSTPGRE
                     GEAWKQFMIRLNYDLVWRTDSPPFEKAPHAWMGSARYSETQELKPATYPFANEEEVLS
                     FDPVAAIGVPDRQETRQLFERNWRATQSDLPFLVVPSGYYNTVFTWCIVTFGWELFMA
                     AAMADPERFDRVLEGFCQLSKPIFEAAAEIPLPAFICHDDIVWTAGAVFHPDWYRRFI
                     FPRYKKLWAPLREAGIPILFCSDGNFTEFVPDIADAGANGFIFEPLTDLEMVAARYGN
                     THVIIGNADCRILTYGSRGDIYAEVKRCADIGRSLPGYFFAVGNHIPYNVPVENAMYY
                     FDLIEELGRR"
     gene            1816..>2417
                     /locus_tag="PLT86_14650"
     CDS             1816..>2417
                     /locus_tag="PLT86_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37899.1"
                     /translation="MRSTPIGIVMLNDEREHVWRMNAAVNMSVVRKWAQIIRKGVKNV
                     DGSSPEVVIGSEIVTGVRSAQKVGEELRRANVKQLILCYNVWNFPFLVWPLLNSVGRD
                     VPVLSLSNNNGEFPGNVGLLATDGALRQAGIRTHRIVGEMDDPKTCQAVFDWVRASQA
                     LTTMRNEVFGIYGGHSMGMETGFFHLVPTVKTFGTTGRQI"
BASE COUNT          539 a          658 c          714 g          506 t
ORIGIN      
        1 gatgggatac actcgcaggg ctgtcgcttt gaaaaggatt gcggagcgcg tcattcgcgc
       61 ctacgatgga cgactcccga gatcaccgga acacctccag actttccccg gtattggcgc
      121 tgccaccgcg ggagcgattt gcgcgtacgc gtacaacttg ccgaccgtgt tcatcgaaac
      181 aaacatcaga cgcgtgttca tacacacgtt tttcggcgaa cacgaaacgg tttcggacaa
      241 gcagatcctg ccgctggtcg aagaaacact ggatcattct caaccgcgag tctggtacta
      301 cgcgctgacg gactatggtg ctgcactcgg acggagactg gagaatccaa accgcaaaag
      361 cgccggttac cggcggcagg gccgttttga gggatcgaaa cggcaaattc gttcgaccat
      421 cgttcgcgaa cttctcgcgc ggggggcgct taccctcggg gatatcgacc agttgtgctc
      481 cgggacgccg ttcgaagggg gctccatact tcgtgaactg caaagggacg gccttgtcca
      541 gccacttggc gaccaatggt gcgttgcgtt cacccccgat tctgccggca ctgtgtgatc
      601 atcttctacc cagttcctca ataagatcga agtagtacat tgcgttttca accggaacgt
      661 tataggggat atggttcccg actgcgaaga aataccccgg gagactgcgc ccgatatccg
      721 cgcaacgttt cacttctgcg tagatgtctc cccgtgaacc gtacgtgaga atccggcaat
      781 ctgcgttgcc gataattacg tgcgtgtttc cataccgtgc cgccaccatt tccaggtccg
      841 tcagaggttc gaaaatgaat ccgtttgcgc ctgcgtctgc gatgtcggga acaaattccg
      901 tgaagttccc atctgaacag aacagaatgg gaatccccgc ctccctgagc ggagcccaca
      961 gttttttgta ccgagggaaa atgaatcttc ggtaccaatc cggatggaat acggcgccgg
     1021 ctgtccacac gatgtcgtcg tggcagatga acgcgggaag cggtatctcc gcggcggcct
     1081 cgaagatcgg tttggataac tggcaaaacc cctcgagaac acgatcaaac cgttcgggat
     1141 cggccatggc tgctgccatg aacagctccc acccgaacgt aacaatgcac cacgtaaata
     1201 ccgtgttgta gtagccgctc ggaaccacaa ggaagggaag atccgactga gtggcgcgcc
     1261 agttccgttc gaaaagctgc ctggtttcct gccggtccgg aacacctatc gccgcaacgg
     1321 gatcaaacga gagcacctcc tcctcgtttg caaacgggta tgtagcgggt ttcagctcct
     1381 gagtctccga gtaccgtgcc gatcccatcc aggcgtgggg cgccttttca aacggtgggg
     1441 agtcggtccg ccacacaagg tcgtaattga gccggatcat gaattgcttc caggcctcgc
     1501 cctcgcgccc tggggtggat ggatctattc cggtgacgtg tttgacgagc gtcgggttgc
     1561 tgacgtattc ggtgtgaggt atcgtgtcgg gcatctgcag tcgtattgcc tggatgccgc
     1621 gttcgtaact catggcgggt ctccttggtt ccggaacggg gtgtcagcgg cggcgaagca
     1681 aagtgtacgc gtcagaatgg aattgggaca aacgggggtt aaccccgggc tcccgagtcc
     1741 ttagattcta tcacgaacag gaacccaaaa gcacgacata cgccaattgc ctgcatttgc
     1801 aggaaggaga tgagcatgcg gagcacacca atcgggattg tgatgctgaa tgacgaacgc
     1861 gagcacgtgt ggcggatgaa tgcggcagtc aatatgtccg tagtgcgcaa atgggcgcag
     1921 atcattcgga agggagtgaa gaacgtagat gggtcgtccc cggaggttgt cattggctcc
     1981 gagattgtga ccggcgttcg cagcgctcag aaggttggcg aggagctgag gagggcaaac
     2041 gtcaaacagc ttatcctgtg ttacaatgtg tggaatttcc cgtttcttgt ctggccgctg
     2101 ctcaacagcg taggacggga tgtcccggtg ctcagccttt ccaacaacaa cggtgagttc
     2161 cccggaaacg taggcttgct cgcgaccgac ggagcattgc gccaggcagg catccggact
     2221 caccgaattg ttggtgaaat ggatgacccc aaaacgtgcc aggccgtgtt cgactgggta
     2281 cgggcaagcc aggcgctcac cacgatgaga aacgaggtat tcgggatata cggtggccat
     2341 tccatgggga tggaaaccgg cttcttccac ctcgtgccga cggtgaaaac cttcggtacc
     2401 accggaaggc aaataga
//
LOCUS       DAOSOO010000341         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_45395_length_2350_cov_3.617865, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000341 DAOSOO010000000
VERSION     DAOSOO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45395_length_2350_cov_3.617865"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PLT86_14655"
     CDS             <1..1959
                     /locus_tag="PLT86_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT37900.1"
                     /translation="QVGTDMFFLAVAIAAATLILERGGWQRSLAAGLMAGFAYLTRYN
                     GVFLTLGGIVIMALADRDTLGGRQRLLRTVAFLGGVLVMFAPWSYYTWSQGLGFFFNA
                     NHLNIAYEMFGRDTVSWDQFWEYFNPAFASFGEVVRGNVGRFVAMLARNSIEHFWWDM
                     RRVLVAGTAPWVVPLSIVWLAMIAYGLVRAVMMKRERAWPAIVLGVGAYGVLVPVFYG
                     ERFSLPMVPIYAVLAATAVTGGLRLSSKSVSLAAIPAICGVLLLWGGIATAKEEVAKL
                     METQPAEVGAVARAVGRSLPEGSAVLARKPHIAYFTGTEFIRVPFLDRIEELPGIALQ
                     HHAQYLFVSAIEAALRPPLTPLLDPRNAPPFLKPIAAVSGAAPAVLYEFTMKIPRKAS
                     VNTTESRQVPGEEAVPQKVRLGRAYLRAGRLDLANEWFQKALADDPSEPTALLGLIQL
                     DEARARWLAQEAQTAGSNSETVSRVLDSVESRLIALAEAAPGSRDVQTAAGEFLMRRD
                     KVPEAIRSFRRVAAMDPPDPDVIGVLGELEESVGNLDGAEHEFARLVGIDPGNATARS
                     ALGRILLARARPGEALRHLQMAYEMDPYSPLTRALLARAYQMQGDSSRAGDHWRGVLA
                     MTPEGDSLHVEAQAALDAISTADRRRLR"
     gene            1956..>2350
                     /locus_tag="PLT86_14660"
     CDS             1956..>2350
                     /locus_tag="PLT86_14660"
                     /inference="COORDINATES: protein motif:HMM:NF039894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT37901.1"
                     /translation="MTTQIEYVDHSGTGFLAGLREGDTVVAVNGHAVRDPLEWRFVIA
                     EERLSIEIARGRERLSVVVEKDYDDEMGVRLREPKFRRCNNKCVFCFVDQNPPGVRRA
                     LRFKDEDFRLSFLYGNFVTLTNTPSSDLER"
BASE COUNT          435 a          633 c          773 g          509 t
ORIGIN      
        1 caggttggta ctgacatgtt cttcctcgcg gtcgcgattg cggcggcgac cctgattctg
       61 gagcgaggag gttggcaacg atctcttgcg gcgggtttga tggccggatt cgcctatctc
      121 acgcggtaca atggagtgtt tctgacgctg ggcggaatcg taatcatggc gttggccgac
      181 cgtgacacac tgggcggcag gcagcgtctg ctgaggaccg tagcgtttct cggcggggtt
      241 ctggtgatgt ttgcaccttg gtcttattac acatggagcc agggcctcgg gttctttttc
      301 aacgcaaacc acctgaacat cgcgtatgag atgttcggac gagataccgt gtcgtgggac
      361 cagttctggg agtattttaa ccccgcattc gcctcctttg gggaagtggt gcggggtaac
      421 gtcgggagat ttgtcgcgat gctggcgcgc aacagcatcg agcatttctg gtgggatatg
      481 cgtcgcgtcc ttgttgcggg aacagcgccc tgggtggtgc cgctttcaat agtgtggctg
      541 gcgatgatcg cctatggcct cgtccgcgcg gtgatgatga agcgcgagcg cgcctggcct
      601 gcgatagttc tgggggtcgg ggcgtacggt gttctcgttc ctgtgttcta cggggagcgg
      661 ttcagtctcc cgatggtgcc gatctatgct gttttggcgg ctacagcagt gacaggcggg
      721 ctaaggctga gttccaaatc cgttagcttg gcggcgatcc ctgcgatttg cggggtgctt
      781 ctcctttggg ggggtatagc taccgcgaag gaggaggtgg cgaaactgat ggaaacccag
      841 ccggcggagg taggcgcagt tgccagggcc gtcgggcgtt ctcttcccga aggctctgcg
      901 gtgcttgcaa gaaagccgca tatcgcgtac ttcaccggca cggagtttat tcgcgtcccg
      961 tttctggatc gcattgaaga actgccgggg attgccctgc agcaccacgc tcaatacctc
     1021 tttgtaagcg ccattgaggc tgccctccgc ccacctctca ctccgctgct ggacccgcgg
     1081 aacgcccctc cgtttctgaa gcccatcgct gcggtttcgg gtgctgcacc cgctgtgttg
     1141 tatgagttca cgatgaaaat cccgcgaaag gcttccgtca acaccacgga gtctcgacag
     1201 gtgccgggag aggaggctgt tcctcagaag gtgaggctgg gcagggcata cctgcgggcg
     1261 gggaggctgg atcttgcgaa cgaatggttc cagaaggccc ttgcggacga tccctcggaa
     1321 ccgactgcgt tactcgggct gattcaactg gacgaagccc gcgcgcgatg gctcgcgcag
     1381 gaagcccaga ctgcgggatc gaactcggag actgtttcgc gtgttctcga tagcgtggaa
     1441 agtcgtctga tcgcgctcgc cgaagctgcg cccggctcac gcgacgtgca aacagcggca
     1501 ggggaattcc tcatgcgtcg cgacaaagtc cccgaggcta tccggtcgtt tcgtcgcgtg
     1561 gccgcgatgg acccgcccga tccagacgtg atcggggttc tcggcgaatt ggaggaatct
     1621 gtcggcaatc tggacggtgc ggaacacgaa tttgctcgac tggtaggtat tgatccaggg
     1681 aatgcgacgg cgcgaagtgc gttgggacgg attctactgg cacgcgcaag acccggtgaa
     1741 gcactgagac atcttcaaat ggcgtacgaa atggacccat attcgcccct cacgagggcg
     1801 ttgttggccc gcgcgtatca gatgcaggga gattcatcca gggctggtga ccactggcgc
     1861 ggggtacttg cgatgacgcc ggagggcgat tccctccacg tggaggcgca ggccgcgctg
     1921 gacgcaatat caacggcaga ccgacgaaga ctccgatgac gacgcagatc gaatacgtcg
     1981 accatagcgg taccggattc ctcgcgggat tgcgggaagg agacaccgta gttgcagtga
     2041 acggtcacgc ggttcgcgac cccttggaat ggcgcttcgt gatcgcagaa gagagactga
     2101 gcattgagat tgcgcgcggg agagagcgtc tttctgtcgt cgtggaaaag gattacgacg
     2161 acgaaatggg cgtccgcctg cgtgagccga agtttcgcag atgcaacaac aagtgcgtct
     2221 tctgcttcgt ggatcagaat ccccccggag taaggcgtgc gttgaggttc aaagacgagg
     2281 atttccggct ttcgttcctc tacggcaatt ttgtcacgct cacgaataca ccctcatctg
     2341 acctcgagcg
//
LOCUS       DAOSOO010000342         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_45745_length_2336_cov_4.947830, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000342 DAOSOO010000000
VERSION     DAOSOO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45745_length_2336_cov_4.947830"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..415)
                     /locus_tag="PLT86_14665"
     CDS             complement(74..415)
                     /locus_tag="PLT86_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOT37902.1"
                     /translation="MPTLFSRIAAGEIPATIVARTSDCIAIEDINPQAPVHVLIVPLK
                     EIPSVNTLAAEDEALVGKMVLLAREIAAQRGLQEGGYRLVFNCGPDAGQTVDHLHLHL
                     LGGRSFGWPPG"
     gene            complement(436..1002)
                     /locus_tag="PLT86_14670"
     CDS             complement(436..1002)
                     /locus_tag="PLT86_14670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT37903.1"
                     /translation="MKNLFRRAGLTSIAVAIVIAAAAAGCSKKESDQTASTEISSETS
                     APRFVSMNAPAPDFTLKNRITGADVKLSNYRGRVVMVNFWAPWCPPCREELPDLVGLY
                     EANRGKGFELLGVFVSGDPGEVDQMVRQHGIDYPVLTGDESVARAWLVNGIPTTYIID
                     REGVVRVQYVGAQPRAVFEQDVTRLLNN"
     gene            1247..2182
                     /gene="cysK"
                     /locus_tag="PLT86_14675"
     CDS             1247..2182
                     /gene="cysK"
                     /locus_tag="PLT86_14675"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT37904.1"
                     /translation="MGRIYANIIETIGNTPLVRINRIMDGAAAEVVAKVEAFNPLASV
                     KDRIGAAMIEAGEKAGKITKDTVLIEPTSGNTGIALAFVCAAKGYRLILTMPESMSME
                     RRALLRALGAEVVLTPAAEGMKGAIKKAEELAKETPHAFVPQQFSNPANPEIHRRTTA
                     EEIWRDTDGKVDILVAGVGTGGTITGISEVIKTRKPSFRTVAVEPAASPVLSGGTAGP
                     HKIQGIGAGFVPDNLNVQIVDEIIPVENDDAMAMARRAAREEGLLVGISSGAALFAAR
                     AVAKREENSGKMIVVILPSFGERYLSTDLFKDLMV"
BASE COUNT          511 a          668 c          672 g          485 t
ORIGIN      
        1 tcatgcctga ccctatcgac ctcaaagtct tctgacaggc cggaatggca cgtccacacc
       61 cggggtcggc cgttcagccg ggcggccagc caaatgagcg cccgccgaga agatggagat
      121 gcaggtgatc caccgtctgg cctgcgtccg gcccgcagtt gaagaccagc cggtatcccc
      181 cttcttgaag cccccgctga gctgcgattt ccctggcgag aaggaccatt ttgccgacaa
      241 gggcctcgtc ctctgccgcc agcgtattta ccgacggaat ctccttcaat ggaacgatca
      301 gcacgtgcac cggcgcctgg gggttgatat cctcgatggc aatgcagtca gacgttcggg
      361 ctacgatggt tgccggtatt tcgccagcag cgattcgtga gaaaagcgtt ggcatctgtc
      421 cctcctggct cccgttcagt tgttcaacaa tctggtcaca tcctgttcaa acaccgcacg
      481 cggctgcgca ccgacgtact ggacgcgcac gactccctct ctgtcaatga tgtacgtcgt
      541 cgggattccg ttgaccaacc atgcgcgtgc cacgctttca tcgccggtaa gaaccgggta
      601 gtcgattccg tgttgacgaa ccatctggtc gacttcacca gggtctccac tcacaaatac
      661 gccgaggagt tcaaatcctt tgcctcggtt cgcttcgtaa agccccacaa ggtccggcaa
      721 ttcctcacgg cacggcgggc accagggcgc ccagaagttc accatgacta ctcgaccgcg
      781 gtagttgctc aatttcacat ctgctccggt aatgcggttc ttcagtgtaa aatccggcgc
      841 aggggcattc atgcttacaa atcgtggggc cgatgtctcg ctgctgattt cagtgctggc
      901 ggtctggtca ctctcctttt tggagcaccc ggccgctgct gcggcaataa cgatggcaac
      961 tgcgatggaa gtcaaacctg cacgtctgaa aaggttcttc atttcgatac ccatctcttc
     1021 gagtccaccc gtgtgtgttt cggtactcct gcgtggagtc gcaaaatgcg caagatgaaa
     1081 aacactggaa ccgcagcgtt ggttcatgcg ccaacggcat tgcaaaagaa tacgttgtgt
     1141 gcgggccaaa gaaaagttga caggctcgcg ggaaccgtaa gaagcccgga tttgttgatt
     1201 cccaatacgt ggtcacggtc gttttccaca ttgcggaggt acccagatgg ggcggattta
     1261 cgcgaacatc atcgaaacga ttggcaacac gccgcttgtc cggatcaatc gcattatgga
     1321 tggagctgct gccgaggtgg tagccaaggt cgaagcgttc aaccctctgg cgagcgtgaa
     1381 agatcgaatc ggtgcagcaa tgattgaagc tggcgagaaa gctggcaaaa tcaccaagga
     1441 cacggtgctg attgaaccga catctggaaa cacgggcatc gccctcgcgt tcgtgtgtgc
     1501 cgcaaaaggg tatcgtctca tcctcaccat gcctgaaagc atgagcatgg aacgccgcgc
     1561 gcttctgcgg gctctgggcg cggaagtagt tcttaccccg gccgcagaag gcatgaaagg
     1621 ggcgatcaag aaagccgagg aactggcaaa ggaaacacca cacgcgttcg taccgcagca
     1681 gttctcaaat cctgcaaacc cggaaataca ccggaggacg accgcagagg aaatctggcg
     1741 tgacactgac ggcaaagtgg acattctggt cgcgggcgta ggcacgggtg gtaccatcac
     1801 cggcatctcg gaggtaatca agactcgaaa gccatccttc cgcaccgtgg ccgttgagcc
     1861 tgcggcgtct ccagttcttt ctggaggcac cgcaggacct cacaaaatac agggaatcgg
     1921 agccggtttc gtaccggaca atctcaatgt gcagattgtt gacgagatca tcccggtgga
     1981 gaacgacgac gcgatggcaa tggcgcgccg ggcggcacgg gaggagggcc tcctcgtcgg
     2041 gatttcctcg ggcgcggccc tttttgccgc acgtgccgtg gcaaagcggg aagagaattc
     2101 cggcaaaatg atagtcgtca tattgccctc gttcggagaa cggtatctga gcaccgacct
     2161 cttcaaagac ctaatggtgt aaatcgtcca gcgcgcttgc tgtccggtcc ggcaattcct
     2221 accttacgcg gaggggacac acgtctcctc cgcttgttca ttcatcgcag acgaggtgcc
     2281 aggcacatgg ctgatatcgg gatggttggt gtgggtgggt acggtgcgac gatcct
//
LOCUS       DAOSOO010000343         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_46716_length_2301_cov_5.095726, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000343 DAOSOO010000000
VERSION     DAOSOO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46716_length_2301_cov_5.095726"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2301
                     /locus_tag="PLT86_14680"
     CDS             <1..>2301
                     /locus_tag="PLT86_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOT37905.1"
                     /translation="ARLVVCPVSVVEGGVRWTKSLRVAVRFASENTVPLARAARQDTD
                     HPAVGALNADLIPLFGRTGTAQPAERVAASQNSYRVRFYVRYEGIYRITGADLKKWTQ
                     GSPIIWETVDPRTFRLEESGREIPIYVEGEERGVVEDSLAIEFFARPNIGRYQSVAPD
                     LYRDPFVEMGIYNLSWGHGPGARLSDEDGSLTTPSSSADLVQCLTYWHTVHAEEDQTF
                     ERFGGANEDLIDRYMWKRIGHGETAEFPIQLESPVNDAKNPPIVEVMARGATFSRHIM
                     EVRLQGTRIGTAGLSPILRDLDLIHFKRNSTPVTLEDGVNTLSVITGDPSNPDPNGND
                     VVALNWFEVTYERNYVAKGNSISFRRPSEPSQGVFEFSLSGFGTPDITVYKVGVSRIR
                     NFTIETPITSRSEGYTIRFQDRIDGDDPRYIAVASSRKLKPDRVAVVPVWERPLTDRT
                     RAADYLIVTHSVLLDSVARFAEYRRSAQGGSHTVAVVDVAQIYDEFGYGYPAAEPVRD
                     FLNYAFRYWAQPSLRYVVLVGDGVMRYPSDNMGLLAYPLIPAFLEAQAKWGGTETDHP
                     YAMLAGDDLLPDIIVGRIPASTTNDLRVAVDKIIEFEQTPERSKWRNSVLLISEGEAW
                     FVAQHEFLGAELPPSYFQKKLYNGATTAPHLDLFYGRRAESLAFLNDGSLWTIYLGHG
                     GGGVWGSDRLLVHADPPTLQNAGRAGIFLSMTCFTGAFAGVTQKSLAELMLFSRGGAI
                     AWLGASSVGWVNNDFYFTQSIIRAAFA"
BASE COUNT          473 a          620 c          700 g          508 t
ORIGIN      
        1 ggcgagactc gtcgtctgcc cggtcagtgt agtggaaggt ggtgttcgct ggacgaagtc
       61 gcttcgcgtc gcggtgcgct ttgcctcgga gaacacggtg ccgctggcga gagccgcgag
      121 gcaagatacc gatcatcctg cggtgggcgc cctcaatgcc gatttgatac cgctattcgg
      181 acgaacgggg accgctcaac ccgcggaacg ggttgcggcg tctcagaact catatcgcgt
      241 gcgtttttat gtccgctatg aggggatcta ccgcatcaca ggcgccgatc tgaaaaaatg
      301 gacacagggc agtcccataa tttgggaaac tgtcgacccc cgcacgttcc ggttggagga
      361 atcgggcagg gagataccca tatacgtgga aggtgaggaa cgtggcgttg tcgaagactc
      421 gctcgcgatt gagttcttcg cgcggccgaa catcggcagg taccagtccg ttgcgccaga
      481 cctgtaccgg gaccccttcg tggaaatggg gatttacaac ctttcgtggg gccacgggcc
      541 gggcgcacgc ctttctgatg aggatggttc tctgacgacg ccgtcttctt cggcagatct
      601 ggttcagtgc cttacctact ggcataccgt tcatgcggag gaagaccaga cgtttgagcg
      661 attcggcggc gcgaatgagg atttgataga tcgttacatg tggaagagga ttggccacgg
      721 ggaaacggca gaattcccca tccagcttga aagccctgta aatgacgcga aaaatcctcc
      781 gattgttgag gtcatggctc ggggtgcgac gttcagccgg catatcatgg aggtccgatt
      841 gcagggaacc cggatcggga cggcaggtct ttcgccgatc ctgagagatc tggatctcat
      901 ccatttcaag cgcaactcaa caccggtcac actggaggac ggagtcaaca cgctctcggt
      961 catcaccgga gatccctcca atccggatcc taacgggaat gatgtcgtcg cattgaactg
     1021 gttcgaggtt acctacgaac ggaactatgt ggcgaagggc aattcgattt ccttccgtcg
     1081 cccttcggag ccctcacagg gggtgtttga gttctctctg tccggcttcg gcacccccga
     1141 tattaccgtg tacaaagtag gcgtgagccg aatacggaac ttcaccattg aaacccccat
     1201 cacatcccgt tctgagggat atacgattcg ctttcaggat cgtattgacg gcgatgatcc
     1261 ccggtacatt gcggtggcct cttcccggaa gctcaaaccc gatcgcgtgg cggtggtgcc
     1321 ggtgtgggaa cgtccgctga cggacagaac gcgcgccgct gattacctta tcgttaccca
     1381 ttccgtgctc ctggattccg tcgcccggtt cgccgaatac cgcaggagcg cgcagggggg
     1441 aagccacact gtggcggttg tggatgtggc gcagatatac gacgaattcg gatatggata
     1501 ccccgccgcg gagccggttc gagatttttt gaattacgcg ttccggtact gggcgcaacc
     1561 ctcgttgcgt tacgttgtgt tggtgggtga cggagtcatg aggtacccgt cggacaatat
     1621 ggggctgctg gcgtatccat tgattcccgc gtttctggaa gcacaggcaa aatggggagg
     1681 tacagagacc gaccacccct acgcgatgct ggcgggcgat gaccttctcc ccgacataat
     1741 cgtggggaga atacctgctt ccacgacaaa cgatctccgt gtcgccgtgg acaagatcat
     1801 cgaatttgag cagactccgg aacgatcaaa atggcgaaac tcggtgttgc tgatttccga
     1861 aggcgaagca tggttcgttg cacagcatga attcctcggt gcagaacttc caccaagcta
     1921 ctttcagaag aaactgtaca acggggccac aacggcgccc catctcgatt tgttctatgg
     1981 aagacgtgcg gaaagccttg cattcctcaa tgacgggtcg ttgtggacga tctatctggg
     2041 acacggggga ggtggcgtgt gggggagcga ccgcctgctt gttcacgcgg acccgcccac
     2101 cctgcagaac gccggcaggg cagggatatt tttgagcatg acgtgtttca caggggcttt
     2161 tgccggagtt acccagaagt cactggccga attgatgctt ttctcccgcg gtggcgcaat
     2221 cgcttggctg ggtgcgtcga gcgtcggctg ggtgaacaat gatttctact tcacccagtc
     2281 gatcattcgt gcggcattcg c
//
LOCUS       DAOSOO010000344         2284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_47176_length_2284_cov_5.505608, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000344 DAOSOO010000000
VERSION     DAOSOO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47176_length_2284_cov_5.505608"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLT86_14685"
     CDS             <1..383
                     /locus_tag="PLT86_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37906.1"
                     /translation="VLGPSPDIWYRNHGAGAIKVFRLGDAFLALENGIYIDTENRTHS
                     AINVLMSEDGIAWQDAPYNPIVVPSGNGWNRSHIYQLDLRWYNGKLWLFYNARDGWSP
                     AREFIGLSTLEWDGPAPEKMWRLR"
     gene            586..1584
                     /locus_tag="PLT86_14690"
     CDS             586..1584
                     /locus_tag="PLT86_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HOT37907.1"
                     /translation="MPFKTRAVVAVSPEKVVLREVTVPDPAPGEVVVRAEYSSISTGT
                     ERWVVTGQFHAPGAKPMPHPMVPGYQKAGLIEALGAEVTGLHVGQRVFATTSRMADDE
                     VLPGWGGHIEHSVLPQREVIPLPEGLDPVKASALVLTQVGYNGGFRPPVEPGASALVI
                     GDGLVGQWLAQALRHRGARVVLAGRRAERLAIAAKWSADVTANVRETPLEEVAKREAP
                     NGFDIVADSVGTRASLEQIIPLAHHESHLVLNGYYREGENLLDIQLLHGREITTHAPA
                     GWTRDRLVATLDLVAKDTMHVRELMTHIMPVERASEAYDLVLRKPQEFLGICLKWR"
     gene            1599..>2284
                     /locus_tag="PLT86_14695"
     CDS             1599..>2284
                     /locus_tag="PLT86_14695"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT37908.1"
                     /translation="MPNTMRALQIVAPNDARVVTVDVPQIGAGDVLVRIKAATVCSHW
                     DLSMLGGWDIFERPGFPKYPISPGWPGHEVAGEVIAVGEGVDAFRPGDHVAAWSSRHS
                     QQEGKLGYYAEYAAIPAEDLLAVPESMPFEEAAILEMAMCVAASIRQAGDLSGRNVAI
                     GGAGAAGLLALQMARSLGARRVDVFEPTESRRKLALTLGAHAAFDPASDDAKALSPRT
                     YEVAFECAGAA"
BASE COUNT          416 a          711 c          768 g          389 t
ORIGIN      
        1 ccgtgctcgg cccgtctcct gatatctggt accgaaacca cggcgccggg gccatcaagg
       61 tgttccgctt aggggatgcc ttcctcgcgc tggaaaacgg catctacatc gacacggaaa
      121 accggacgca ttccgccatc aacgtgctga tgtcggagga cggcatcgcg tggcaggacg
      181 cgccgtacaa ccccatcgtt gtgccatcgg gcaacgggtg gaacaggtcg cacatctacc
      241 agcttgatct caggtggtac aacgggaagc tctggctttt ctacaacgcc cgcgacggct
      301 ggagccctgc gcgcgagttc atcggccttt ccacgctgga gtgggacggc cctgcgccgg
      361 agaagatgtg gcgtctgcgg tgaaccgggc cggacggagc gggcccctcc acgttgagcg
      421 tctccggcgc ggtggctcat gccgcgtccg gcgcggccag cctgtcgaag ccccgcgcaa
      481 ggagccgcgg gaaagccctg gcggcatgcc tccctccggc cggtgtccag gggtatcttt
      541 cggtacgtct ggacaactcc gaacaactca acaaggacaa ccaccatgcc attcaaaaca
      601 cgcgctgtcg ttgcggtgtc cccggaaaag gtcgtactgc gcgaagtaac ggtaccggat
      661 cctgcaccgg gcgaagttgt tgtcagggcg gagtattcct ccatcagcac gggaacggaa
      721 cgatgggtag ttaccgggca attccacgcc cccggcgcaa aaccgatgcc gcaccccatg
      781 gttcccgggt accagaaggc gggcctgatc gaagcactcg gcgcggaggt gaccgggctt
      841 cacgtggggc agcgggtgtt cgcaacgacc tcccggatgg cggacgacga ggttctgccc
      901 ggctggggtg ggcacatcga acactccgta ctgcctcagc gtgaggtcat tccccttccg
      961 gaagggctcg acccggtgaa agcctccgcc ctcgtcctga cgcaggtagg ctacaacggc
     1021 gggttccgcc cccccgtgga accgggtgcc tccgcgctgg ttatcgggga cgggctggtc
     1081 ggccagtggc tggcgcaggc gcttcgacat cgcggcgcgc gggtggtgtt ggcggggcga
     1141 agggcggagc ggctcgcaat cgcagcgaaa tggtcggcgg acgtgacagc gaacgtgcgc
     1201 gaaacccctc ttgaggaggt ggcgaaacgg gaagcgccga acggattcga tattgtggcg
     1261 gattccgtgg gtacacgcgc cagcctcgaa cagatcatcc cgctggccca ccacgaaagc
     1321 caccttgtgc tgaacggcta ctaccgtgag ggcgagaacc tgctggatat ccagctcctt
     1381 cacggccggg agatcaccac tcacgccccc gccgggtgga cacgcgatcg gctcgtcgca
     1441 acgcttgatc tcgtcgcaaa agacacgatg catgtgcgcg agttgatgac ccacatcatg
     1501 ccggtggaga gggcttccga agcatacgat cttgttctcc gaaaaccgca ggaatttctc
     1561 gggatttgcc tgaaatggcg gtgacgatgg aggcgacgat gccgaacact atgcgtgcat
     1621 tgcagatagt cgcgccgaac gacgcacggg ttgtcacggt ggatgttcca cagatcggcg
     1681 cgggagacgt gctggtcagg atcaaggccg cgacggtctg ctcccactgg gacctgagca
     1741 tgctcggcgg gtgggatatc tttgagcgtc ccggcttccc caaatacccg atctcgcccg
     1801 gctggcccgg ccatgaagtg gcgggcgaag tgattgccgt gggggaaggc gtggatgcgt
     1861 ttcgcccggg cgaccatgtg gcggcgtgga gttcccgcca ttcgcagcag gaggggaagc
     1921 tcggatatta cgcagaatac gccgccattc ccgcggagga cctgcttgcc gttcccgaat
     1981 ccatgccgtt tgaggaagcc gccattctgg agatggccat gtgcgtggcc gcttccatcc
     2041 ggcaggcggg tgacctctcg ggcaggaacg tcgcaatcgg cggcgcaggg gcggcgggcc
     2101 tgctcgcgct ccagatggcg cgttccctcg gtgcgcgccg cgtggacgtt tttgagccca
     2161 ccgaaagccg cagaaaactc gcccttacac tgggagcgca tgcagcgttc gacccggcct
     2221 ctgacgacgc gaaggcactc tcgccgcgaa catacgaggt cgcgttcgag tgcgcgggag
     2281 cggc
//
LOCUS       DAOSOO010000345         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_47364_length_2277_cov_3.700270, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000345 DAOSOO010000000
VERSION     DAOSOO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47364_length_2277_cov_3.700270"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..774)
                     /locus_tag="PLT86_14700"
     CDS             complement(100..774)
                     /locus_tag="PLT86_14700"
                     /inference="COORDINATES: protein motif:HMM:NF020785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HOT37909.1"
                     /translation="MGLLTPRGQTLFHETFDEGLTQWRLEGIGSPRVVENARGERALR
                     LDSVGSIQGAEGCNFFHVKDFPDSIVLDFDLYVHFSDGLFITFVAVRGLNGEDMFSLP
                     PRSGVFGDYVAKDALLRSYHVSVSRYNDKGEHTGVSNWRRNPGLHLMAQGPDPCRQIK
                     TFYHLTVVKAGPHLQLGVNGVLAHEFVDPQTLPIPLPDSGKIGFRAIGSQIVVDVSNI
                     RVTSLD"
     gene            1060..2220
                     /locus_tag="PLT86_14705"
     CDS             1060..2220
                     /locus_tag="PLT86_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37910.1"
                     /translation="MEKTIGVGMIGYAFMGRAHSNGWRQVGHFFDLPMKPVMKTICGR
                     NKAAVTAAAKTMGWEKAETDWMKMIKDPQIQIVDICTPGNEHKPMALAALKAGKHVVC
                     EKPLANTLAEAKEMAHAAKEAGVCTLTAFNYRRVPAIALAKQMIDQGLLGTIYHWRAV
                     YLQDWIIDPKFPLVWRLRKEVAGSGPHGDLNAHITDLARYLVGEITEVVGMGKTFIKT
                     RPIQEALADGGLGAKAGTKKGKVTVEDAMLFLAKFENGALGSFEATRFAAGRKNFNSF
                     EINGSKGSIAFNLERLNELQYFNRKDDANLQGFRTILCNEGGQPYNAAWWPSGHIIGW
                     EHTFTHEFKDFIEAIAAKKDTMPNFEDGMKTQAVLEAVMVSADKKKWVKVPL"
BASE COUNT          525 a          616 c          675 g          461 t
ORIGIN      
        1 actccctctt cggtctgcac gacgacgaaa cacttgccgt ggacgacgta tgaagaaaca
       61 cttctgatgt tcatggttcc cctcacggtc aggtgctgat cagtcaagag aagtcacccg
      121 gatgttgctg acgtccacca cgatctgaga gccaatagca cgaaacccga tcttgccgct
      181 gtcggggaga ggaattggca gcgtctgcgg gtctacgaac tcgtgggcga gtactccgtt
      241 cacgccgagc tgaagatgtg gtcccgcttt gacgaccgta agatggtaga acgtcttgat
      301 ctgccggcag gggtccggac cctgtgccat cagatgcaac cccggattcc tcctccagtt
      361 ggagactccg gtatgttcgc ctttatcgtt gtaccggctg acagacacgt ggtaagaacg
      421 gagaagagcg tctttggcca catagtctcc gaacactcct gaacgcggtg gcagcgaaaa
      481 catgtcctct ccattcaggc cgcgcaccgc gacaaaggtg atgaagagcc cgtcgctgaa
      541 atgaacgtag aggtcaaagt ccagcacgat actgtccggg aagtccttga catggaagaa
      601 attgcatccc tctgcaccct gaatgcttcc caccgagtca aggcgcaagg cgcgttctcc
      661 ccgcgcattc tctaccacgc gaggactccc aattccttcg agccgccatt gcgtgagccc
      721 ttcatcgaag gtctcgtgaa aaagagtctg tccgcgaggt gtgaggagac ccatagccgc
      781 gctcccacgg ttttttggaa agggggaaca ttggttccgc ccgagattac cgaaatatag
      841 aaaacgtgtt ccgtgcgcag aacccgtggg acagaaatca cccttcttga cgcttatctt
      901 gttctcggcc tatattccca attcagcgcc ggagaggggt tcttgcgccg catacggcgg
      961 cgcggcgtgc ctcactccga gttgcggtcg gttgttccct cagattcttg caactatctc
     1021 gttggtgtgt ggtgcttttt tcctgcggag gatgggcaca tggaaaagac cattggtgtc
     1081 gggatgattg gttacgcgtt catggggcgc gcccactcga acggatggcg tcaggtcggg
     1141 cactttttcg atttgccgat gaagcctgtg atgaagacga tttgcgggcg gaacaaagcg
     1201 gccgtgacgg ccgcggcaaa gaccatgggg tgggaaaagg ccgaaaccga ctggatgaag
     1261 atgatcaagg atccgcagat tcagatcgtt gacatctgca ctcccggaaa tgaacacaaa
     1321 ccgatggccc tcgccgcctt gaaagccggg aagcatgtgg tgtgtgagaa acctctcgcg
     1381 aacaccctgg ccgaggcgaa ggaaatggcg cacgcggcga aagaggccgg tgtgtgcacg
     1441 ctcaccgcgt tcaattaccg tcgggttccc gcaatcgcgc tcgccaaaca gatgatcgac
     1501 cagggcctgc tcggcacgat ctaccactgg cgtgcagtgt acctgcagga ttggatcatt
     1561 gacccgaagt ttcccctcgt gtggcgattg aggaaggaag ttgccggttc tgggccccac
     1621 ggcgatctga acgcgcacat caccgatctc gcccggtacc tggtgggcga aatcacggaa
     1681 gtggtgggta tgggcaaaac attcatcaaa acccgtccca ttcaggaggc gttggcggac
     1741 ggcgggctcg gtgcaaaggc ggggacgaag aaggggaaag ttaccgtcga ggacgcgatg
     1801 ttgttcctgg cgaagttcga gaacggggcg ttgggatcgt ttgaagccac ccgcttcgcg
     1861 gcgggaagga aaaacttcaa ttccttcgaa atcaacggct cgaaaggttc catcgcgttc
     1921 aatctggaac gcctgaacga gcttcagtac ttcaatcgca aagacgatgc gaatctccag
     1981 gggttccgca ccattctctg caacgaaggc gggcagcctt acaacgcggc gtggtggcct
     2041 tcaggtcaca tcatcgggtg ggaacacacg ttcacccacg agttcaagga tttcatcgag
     2101 gccattgcag cgaaaaagga cacaatgccg aatttcgagg acggaatgaa aacgcaggcg
     2161 gttcttgaag cagtcatggt ttccgcagac aagaaaaaat gggtaaaggt accactctga
     2221 acgggcgctt gaataacgcc cggaatgtcc ggaacgcagg gatgccgttc gccggca
//
LOCUS       DAOSOO010000346         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_47721_length_2265_cov_5.433484, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000346 DAOSOO010000000
VERSION     DAOSOO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47721_length_2265_cov_5.433484"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLT86_14710"
     CDS             <1..1005
                     /locus_tag="PLT86_14710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT37911.1"
                     /translation="SECVIAHTHSSKAGVLGRLAARVANVPLIVHTFHGLPYTQTSPV
                     LQRVGYEMIERCAGRISSGTFSVTHDILRRLKEARIGVPDRSWLARSGMDLDRFLKPG
                     PGREEVRRRWGVRSDDVLLGMVGRVHTGKGQDVIVRLAPEILTDAPSVKIAIIGTGPL
                     KDPLQEQVARAGLGDRVLFVGGIPVDEMPEAISALDVLVHVSEREGLARVIVQALACG
                     KPVISYALDGSPEVIADRANGRLIPPGDDNALRNAIAELARNPQLRDEWGAKGPAAVD
                     PEFRAETMIEQIEAGYRDLLQHAGIGCSTSSGVRPFAEWLPALRETPVPQGSMSLGHS
                     "
     gene            992..1897
                     /locus_tag="PLT86_14715"
     CDS             992..1897
                     /locus_tag="PLT86_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004586510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT37912.1"
                     /translation="MDTHNHGGRGTYDYTRAFVIGIALNITFVAAEAAFGVIAGSLAL
                     VADAGHNLSDVLSLGLAWGAAALAARRSTDRRTYGFKRATIYSSLLSAILLLIALGGI
                     SWEAIRRLSHPRPVDGNIVVIIALVGVAINTATALLFARGRHDDLNIRGAFLHMAADA
                     GISLGVVITGLAVTVTGWNWLDPLISIAIVIVIAIGTWGLLRESVNLSLDAVPEGINL
                     REIESYLRNLDQVKDIHDLHVWALSTTETAMTVHVVTTSEALDNGVVADIQTYLHEHY
                     GIRHATIQLESDDGSLKTLNCRECN"
     gene            1950..>2265
                     /locus_tag="PLT86_14720"
     CDS             1950..>2265
                     /locus_tag="PLT86_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37913.1"
                     /translation="MRRWQDITITLISDVVSSLVAFAVALAVRNASASDYLPFPLSAY
                     VVPALLLSLAWAGLFAYVGLYYDWSRKGRFDELVLLTKAVLFGGVVIFLLTFDPNSPL
                     PPT"
BASE COUNT          435 a          660 c          680 g          490 t
ORIGIN      
        1 tcggaatgtg tgatcgccca tacgcacagc tcgaaggcag gggttcttgg tcgtctggcc
       61 gcacgcgtcg caaacgttcc gttaatcgtg cacacgttcc acggtttgcc gtacacacaa
      121 acctcccccg ttctgcagcg cgtcgggtac gagatgatcg aacgctgcgc ggggaggatc
      181 tcgtccggaa cattttcggt cacgcacgac atcttgagac gcttgaagga agccagaatc
      241 ggagtccccg accgttcatg gcttgcacgg agcgggatgg atttggaccg tttcctgaaa
      301 cccggtcccg ggcgcgagga ggttcgtagg cgctgggggg tgcgttcgga cgacgttctt
      361 ctcggcatgg tcgggagagt gcatacgggg aaagggcagg atgttatcgt ccgcctcgct
      421 cccgaaatac tgacagacgc tccgagcgtg aaaatcgcta tcataggtac gggaccgctg
      481 aaagatccgt tgcaggaaca ggttgcccgt gcagggctgg gcgacagggt attgttcgtg
      541 ggcggcattc ccgtggatga gatgccggaa gccatatctg cgttggatgt actggtgcac
      601 gtctccgaac gcgagggtct ggcaagggtt attgtccagg ctctggcgtg cggcaaaccg
      661 gtgatcagct acgccctcga cggatctccc gaagtcattg ctgaccgtgc aaatggaagg
      721 cttatccctc caggcgacga caatgccctg cgcaacgcca tcgctgagct cgcccgcaat
      781 ccgcaactcc gcgacgagtg gggagcgaaa ggtcctgccg cagttgaccc ggaattccgc
      841 gcggaaacca tgatcgaaca gatagaggcg ggttaccgcg accttttgca gcatgccggc
      901 atcgggtgtt ccacttcgtc aggggtgcga ccattcgctg aatggctgcc cgcgttgcgc
      961 gaaacacccg taccacaggg gagcatgtca cttggacact cataaccatg gcggccgcgg
     1021 tacctacgat tacacccgcg cattcgtaat cggaatcgcc ctcaatatca ccttcgtcgc
     1081 ggccgaagcg gcattcggag tgattgcggg ctcgctggcg cttgtcgccg atgcgggcca
     1141 caacctgagt gatgtgttga gccttggtct cgcgtggggt gcagccgcgc tcgcggccag
     1201 aagaagcact gacaggcgga catacgggtt caagcgagcc acaatctatt cctcccttct
     1261 gagcgcaatc ctcctcctca tcgcccttgg aggaatctcg tgggaagcaa tccggcggct
     1321 ttcccatccg cggccggtcg acggcaacat cgtcgtgatt attgctctcg tcggcgtggc
     1381 gatcaacacc gcgactgcgt tgctcttcgc gcggggacgt catgatgacc tcaacatccg
     1441 gggggcgttt ctccatatgg cagccgacgc cggtatttcc cttggcgtcg taataaccgg
     1501 cctggccgtc acagtgacag ggtggaactg gctggacccg ctcataagca ttgcgatagt
     1561 catcgtgatc gcaattggaa catggggcct tttgcgggag tcggtcaatc tatcccttga
     1621 tgcggtcccc gaaggtatca atctgcggga gattgagagc taccttcgga accttgatca
     1681 ggtgaaagac atccatgatc tgcacgtgtg ggcgttgagc acgacggaaa cggcgatgac
     1741 tgttcacgtc gttacgacgt ccgaggctct ggacaacggg gtggtagctg acattcagac
     1801 gtatcttcac gaacactacg gtatccgcca cgctaccatt cagctcgaga gcgacgacgg
     1861 tagtctgaaa acactcaatt gccgggaatg caactgatct gcatgggcga tttcgcactg
     1921 cgccacccgg tgggaactct ggtaacggca tgcgtcgctg gcaagatata accatcacgc
     1981 tgatttcgga tgtcgtgtcg tcgctggtgg cgttcgctgt cgcccttgct gttcgcaacg
     2041 cgtccgcgtc tgattacctt cccttcccgc tctccgcata cgtggttccc gcactgcttc
     2101 tctccctcgc atgggctggt ctcttcgcgt acgtgggcct gtattacgac tggagccgta
     2161 aaggacgctt cgacgaactg gttctgctca cgaaagccgt cctgttcggt ggggttgtga
     2221 tattccttct cacgtttgat ccgaacagcc cacttccgcc aaccc
//
LOCUS       DAOSOO010000347         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_48327_length_2243_cov_3.809415, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000347 DAOSOO010000000
VERSION     DAOSOO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48327_length_2243_cov_3.809415"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..585
                     /locus_tag="PLT86_14725"
     CDS             151..585
                     /locus_tag="PLT86_14725"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOT37914.1"
                     /translation="MNIIAKGDVSEIICLGFARGDYFLEGIVEAAKREKIVHGAVISG
                     MGTFDKCRMHFTTNPGLPPHDEFVELEGALELVSVHGMIVNGKPHLHMTVGDMQRTWA
                     GHLEEGCRVLYLAEVVVARFRDLPLERAEREYGIRELVRATG"
     gene            609..2006
                     /locus_tag="PLT86_14730"
     CDS             609..2006
                     /locus_tag="PLT86_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT37915.1"
                     /translation="MNDRRQKPNIILINCDDLGYGDLGCYGSTRNDTPYIDRLAAGGV
                     RFTDFYMASPVCSASRAAMLTGCYPQRIGFGNYQVLFPGQAQGLNPKERTIARQLKEV
                     GYDTKIVGKWHCGDQPEFLPTRHGFDEYFGIPFSNDMGRQANNAFKHPPLPLLRNEEV
                     IQQQPDQRAITERYVDECLQFMRRKRENPFFLYLAHMYVHVPLFVSKQFLESSRNGAY
                     GGAVACIDWATGMIDAELSRLGIRDETVVIFTSDNGSRARDEGGSNAPLRGTKAQTWE
                     GGQRVPCIMRWPGKIAGGTTCEGIAASIDLFATLAELTGAPLPPDTAIDSISLADTVL
                     HGKPARRTTFAYYKGANLEAVRKGDWKLHFCKTERTPGGPESNVIAELYNLRDDIGET
                     TNVYEQHPDIVREIEAEAEEFRRLFGDQRLNRTGSEVRDIGVCSDPKPLTEYDENHPY
                     MIAYYDLADMPTMAG"
BASE COUNT          497 a          646 c          709 g          391 t
ORIGIN      
        1 tggagggacg cggtctctcg cgtccgttgc cggcacactc tctcaatgga tccataggag
       61 aaagcgccgt cccgcaacga aattgtgtac gtacgtacgg cggaacgtaa cccacatcct
      121 cacccgcttc actccgggag ggccagcgcc atgaatatca tcgcgaaggg tgacgtatcg
      181 gagatcatct gcctcggatt cgcgcggggc gactacttcc tcgagggcat cgtcgaagcg
      241 gcgaaacggg agaaaatcgt ccacggcgcc gtcatttccg gtatgggcac gttcgacaaa
      301 tgccgcatgc atttcaccac caaccccggc ctgccgccgc atgacgagtt tgtcgagctg
      361 gaaggcgcgc tggaactcgt cagcgtgcac gggatgattg tcaacggtaa accgcacctg
      421 catatgacgg tgggcgacat gcagcggacg tgggcggggc atcttgagga aggctgccgc
      481 gtgctctacc tcgcggaggt cgtcgtcgcg cgattccgtg acctgccgtt ggagcgcgcg
      541 gaacgggaat acggcattcg agagctcgta cgggcaaccg ggtagggcag ggaaaaggga
      601 attcacccat gaacgacaga cggcagaagc cgaacataat cctgatcaat tgcgatgacc
      661 tcggctacgg ggaccttggc tgctacggct ccacccgcaa tgacacgccg tacattgaca
      721 ggctcgccgc gggaggtgtg cggttcacgg atttctacat ggcatcgccg gtgtgctcgg
      781 cgtcccgcgc ggcaatgctg acgggctgct acccacaacg gataggcttc ggcaactatc
      841 aggtgctctt tccggggcag gcgcagggcc tgaacccgaa ggagcgaacg atcgccaggc
      901 agttgaaaga ggtcggctac gacaccaaaa tcgtggggaa atggcactgc ggcgatcagc
      961 cggagtttct tcccaccagg cacgggtttg acgaatactt cgggattccg ttcagcaacg
     1021 acatgggccg ccaggcgaac aatgccttca agcacccgcc gttgccgctc ctgcgcaacg
     1081 aggaagttat tcagcagcag ccggatcagc gcgccatcac cgaacggtat gtggatgagt
     1141 gtctgcagtt tatgcgccgc aaacgagaga atccgttctt tctgtacctg gcgcacatgt
     1201 atgtccacgt gccgctgttc gtctcgaagc agtttctcga gagctcgcgc aacggggcgt
     1261 acggcggcgc cgtcgcctgc attgactggg cgaccggcat gatcgatgcg gaactgagcc
     1321 ggctgggcat ccgagacgag actgtggtca tcttcacctc cgacaacggg agcagggccc
     1381 gggatgaggg cggcagcaat gcgccactac gcggcaccaa agcgcaaacg tgggagggcg
     1441 ggcaacgggt tccctgcatc atgcgctggc cggggaaaat cgccgggggg acgacatgtg
     1501 aagggatcgc cgcttcaatt gatctcttcg ccactctggc ggaactgacc ggcgcgcctc
     1561 tgccaccgga cactgcgatt gacagcattt ctctggcaga tactgttctc cacggaaagc
     1621 ccgcccgccg cactaccttt gcgtattaca agggagccaa cctcgaagcc gtgcgaaaag
     1681 gcgactggaa actccatttc tgcaagactg aacggacgcc gggtggtccg gaaagcaatg
     1741 tcatcgcgga actctataac ctgcgcgatg atatcggtga aaccaccaac gtctatgaac
     1801 agcatcccga tatcgtcagg gagattgagg cggaggccga ggagtttcgg cggctgtttg
     1861 gcgatcagcg gctgaatcgc acggggagcg aagttcgtga tatcggtgta tgcagtgatc
     1921 cgaaaccatt gacggaatac gacgagaatc atccctacat gatcgcgtac tacgatctgg
     1981 ccgacatgcc gaccatggcc gggtgaacgg acatcgggaa agtgaactca cgagagccac
     2041 aggatatcgg aaggaaggag acacacaatg ccagcagacc tgcgggacaa gatattcggg
     2101 tgcatcgccg cgtcgcagat cggctcctca atgggggccg cggtggaggg catgcacatg
     2161 gacgagatcg cgaagaaata cggcagagtg gagacgctca tgccgtacgc ccactacgac
     2221 aagcgctggc tgcgccccgc cgg
//
LOCUS       DAOSOO010000348         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_49427_length_2206_cov_4.661088, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000348 DAOSOO010000000
VERSION     DAOSOO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49427_length_2206_cov_4.661088"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..474
                     /locus_tag="PLT86_14735"
     CDS             211..474
                     /locus_tag="PLT86_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT37916.1"
                     /translation="MDSVTSTEARDQFAEHLNQVAYRGARIPIRRRGKIMAYLVPPKD
                     IEALEALEDALDAADAAKALQEFEASGEEAPLYRRVRAILGLV"
     gene            471..728
                     /locus_tag="PLT86_14740"
     CDS             471..728
                     /locus_tag="PLT86_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT37917.1"
                     /translation="MMYEVRITPAALKQLARLPKAARSRIDAHLLALAENPRPHGAKK
                     LEGSHDLYRIRVGDYRVLYYVRDAVLLVLVVKVGQRGGFYK"
     gene            778..2109
                     /locus_tag="PLT86_14745"
     CDS             778..2109
                     /locus_tag="PLT86_14745"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOT37918.1"
                     /translation="MAYRFGCAEQPLPDGVGLDLCGFLARNNPSTGQRDPLHVRALCL
                     DDGTTKIVWAAADVLAISAQSSARIRREAARRFGVRPAHVTVSATHTHSAPGTVFLAN
                     CGTTDRGWLRTWEARVVETIGDAIRNTGTRVHAEFAATDASGFARNRRTLKRDGSLTM
                     GVVPPEDTVHRGPENHALSVLTLRDAATDRPVAALIHFACHAVLFCDQLITADWPGRM
                     LATLKREMGGGFFPMFIQGACGDINPANLSGNPQDNLTRMGDGLAEKALECIHRAGER
                     QPVRLGARLGGARLPWRSLPTEEALRERLLAPEHWHTATREQQAVHLTAQRLIAMMRR
                     GILRAWATMPVQAVRAGNVAIAALGGEILTQTGLHLAAAFPGHHLLCAAYANGSVGYL
                     PPAEEHEYGGYEVERAHVYYGNPDAFAPEAEAIARATAENMVRSVMADRRR"
BASE COUNT          402 a          733 c          716 g          355 t
ORIGIN      
        1 cgacgcgcaa tacgggagtg tggcgacagg gatacgcgtc accatttttc atgcgtcccg
       61 gggacttcag aaaggtggat attcatgcca ttagcctatc gcttcggctg tgcggaacag
      121 ccgcaaccct ccctttgacg tctcacccaa aaagcgtaca tttcgtacta cacgtacaaa
      181 atgtacattg gcttgggtga ggtgacggga atggattcgg taaccagcac ggaagcacgt
      241 gaccagtttg cggagcacct caatcaggtc gcgtacaggg gagcgcgcat cccgatcaga
      301 cggcggggca agatcatggc gtatctggtg ccgccaaagg acatcgaggc gttggaagct
      361 ctcgaagatg ccctcgatgc cgcagacgcc gcgaaagcgt tacaagaatt cgaagcgagt
      421 ggtgaggaag caccccttta tcggcgtgtc cgcgcgattc tgggcctcgt ttgatgtatg
      481 aggttcggat caccccggcg gcgttgaagc aacttgcgcg gctgccgaaa gctgccaggt
      541 cgaggattga cgctcatctc ctcgctctgg cggagaatcc ccgcccgcat ggcgccaaaa
      601 aactcgaagg cagccacgat ctctaccgca tccgggtggg ggattaccgc gtcctctact
      661 acgttcgaga tgcggttttg ttggtcctgg ttgtaaaggt cggacagcgg ggtggcttct
      721 acaagtgacc gaagggcgcc tctccggcgc tcccgagaga aaatgcagga tatgccaatg
      781 gcctatcgct tcggctgtgc ggaacagcca ctgccggacg gcgtcgggct tgatctgtgc
      841 ggcttcctcg cgcggaacaa cccgagcacc ggccagcgcg acccgctcca tgtccgcgcg
      901 ctctgcctcg acgacggcac cacgaagatc gtctgggcag cggcggatgt cctcgcgatt
      961 tccgcgcaat ccagcgcgcg catcaggcgg gaggcggccc gacgattcgg cgtgcggccg
     1021 gctcatgtta cggtatcggc cacacacacc cacagcgccc ccggaacggt gtttctcgcc
     1081 aactgcggca cgaccgaccg cggctggctt cgcacgtggg aagcgcgcgt ggtggaaacc
     1141 atcggcgacg ccatccggaa caccggcacg cgtgtacacg ccgagttcgc cgccaccgac
     1201 gccagcggct tcgcccgcaa ccggcgcacg ctgaagcggg acggctccct caccatgggc
     1261 gtggtgccgc ccgaagacac cgtccaccgc gggccggaga accacgccct ttccgtgctg
     1321 acgctgcgcg acgccgccac agaccgcccg gtggccgcgc ttatccactt tgcgtgccac
     1381 gcggtgctgt tttgcgacca gctcatcacc gccgactggc cgggccgcat gctcgccacg
     1441 ctgaaacggg agatgggcgg cggattcttc ccgatgttca tccagggcgc ctgcggggac
     1501 atcaaccccg cgaacctctc cggcaatccg caggacaacc tcacccgcat gggcgacggg
     1561 ctggccgaaa aggcgctcga gtgcatccac cgcgcgggcg aacggcaacc ggtgcggctg
     1621 ggcgcccggc ttggcggcgc gaggctcccg tggcgctccc tgccgacgga agaggccctg
     1681 cgggagcggc tccttgcccc ggaacactgg cacaccgcaa cgcgcgaaca gcaggcggtt
     1741 cacctcaccg cacaacgcct catcgccatg atgcggcgcg ggattctccg cgcgtgggcc
     1801 accatgccgg ttcaggccgt ccgtgcgggt aatgtggcga tcgcggcgct cggtggcgag
     1861 attctgacac agaccgggct gcatctggcg gcagcgttcc ccgggcacca cctgctctgc
     1921 gccgcgtacg caaacggcag cgtggggtac ctccctcccg cggaggagca tgaatacggt
     1981 ggctacgagg tcgagcgggc gcatgtgtac tacggcaacc cggatgcctt cgcgcccgaa
     2041 gcggaggcaa tcgcccgcgc aaccgcggag aacatggtcc gttctgtaat ggccgaccgg
     2101 cggcgatgag cgaggtgggg cggcatcgcc aaagccgccg cggtggtctc aatacaccgg
     2161 accgcacacc gtaggggcga cgcatgcgtc gcccctgcct gcacgc
//
LOCUS       DAOSOO010000349         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_49712_length_2197_cov_5.466387, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000349 DAOSOO010000000
VERSION     DAOSOO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49712_length_2197_cov_5.466387"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLT86_14750"
     CDS             <1..810
                     /locus_tag="PLT86_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOT37919.1"
                     /translation="NADMGITGANFAAADTGTVIQVTNEGNGRLCATWPPVQVVFMGI
                     EKIIPRLEDLPVFLKLLARSATGQPMTVYTNLITGPRREGEGDGARELHVILIDNGRT
                     AVLGSEYRQSLRCIRCGACLNTCPVYRNVGGHTYGSVYPGPIGSVLSPLYEGRSRFSA
                     LPHASSLCGACYQACPMKINIPHMLTAMRAEQAERGDNHVLERLGMRLWALAMRSPLL
                     YRFGGTALRWGMLPLSSEGWVSSLPYPFSGWTKRRDFPLPAKKSFRGSFKE"
     gene            890..1849
                     /locus_tag="PLT86_14755"
     CDS             890..1849
                     /locus_tag="PLT86_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT37920.1"
                     /translation="MKPLLLHYYVTYRCNARCSFCTIWDPAKWDHRATPSWDQMSSNL
                     TVARKLGTRFVDFTGGEPLLYHDLPRALRLAKVLGFRTSVTTNCLLYPARAKDLAGQV
                     DLLHFSLDAAEKETHDRIRGVPCFDRVMESLDIARALGEHPDILFTATEETFEQIAPL
                     AAIAQAYNTMLIVNPEFAWAGNKGVGERELACLERFARERNVYVNRAFHRLIRAGGNQ
                     RAHPVCHAVEAVVVISPDDHLLLPCFHRQRERIPINGQLEKVRAGSVVKAYKGEDGRQ
                     PYCQGCTINCYMDPSFQYACNAYLFLSLAAKAKYAWSKYVLLA"
     gene            complement(1992..>2197)
                     /locus_tag="PLT86_14760"
     CDS             complement(1992..>2197)
                     /locus_tag="PLT86_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37921.1"
                     /translation="VTSTLPHGSVEDVRKDVKRCIDIGARRGGRFFLAPSSSVGPEVP
                     TENIVELFRFGREYGLEAYGKGR"
BASE COUNT          436 a          768 c          570 g          423 t
ORIGIN      
        1 aacgccgaca tgggcatcac cggcgccaac ttcgccgccg ctgacaccgg cacggtcatt
       61 caggtgacca acgaaggcaa cggccgcctc tgcgccacgt ggccgcccgt ccaggttgtg
      121 ttcatgggga tcgagaaaat catcccccgg ctggaagacc tccccgtctt cctcaagctc
      181 ctcgctcgca gcgccaccgg acagcccatg accgtctaca ccaacctcat caccggccct
      241 cgaagggaag gcgaaggtga cggcgcgcga gagctccacg taatcctgat cgacaacggt
      301 cgcaccgctg ttctcggatc ggagtaccgg cagagcttgc ggtgcattcg atgcggcgcc
      361 tgcctgaata cctgccccgt ctaccggaac gtcggcggcc acacatacgg aagtgtctat
      421 cccggaccca tcgggtcggt gctttcgccc ctgtacgaag gacggagccg cttcagcgcc
      481 cttccccacg cgtcatctct gtgcggagcc tgttaccagg catgcccgat gaagatcaac
      541 atcccgcaca tgttgaccgc catgcgtgcc gaacaggcag aacggggaga caaccacgtc
      601 ctcgaacggc tcggcatgcg cctctgggcg cttgccatgc gctctccact tctttaccgg
      661 ttcggaggaa cagccctccg ctggggaatg ttgccgctct caagcgaggg atgggtatct
      721 tccctccctt acccgttctc cggatggaca aaacgccgcg atttcccgct tcccgcgaaa
      781 aaaagcttcc gcgggagctt caaagaatga actcggcaac cgccttggag gggcgcgctt
      841 cgtcatgccc tttgagctcc ctcgcgcccg ctgatcctcc cgctgcaaaa tgaaaccgct
      901 cctgctccac tattacgtga cctaccgctg caacgcacgg tgctcattct gcaccatctg
      961 ggacccggca aaatgggacc accgcgccac accctcctgg gaccagatga gctccaacct
     1021 caccgttgcc cggaaactgg gcactcgatt cgtagatttc accggcggcg aacctcttct
     1081 gtatcacgac ctgccccgcg cgcttcgcct cgcaaaggtg ctcgggttcc gtaccagcgt
     1141 caccaccaac tgtctgctct accctgcccg tgcaaaagat ctcgccggcc aggttgacct
     1201 gctccacttc tcgctcgacg ccgcggaaaa ggaaactcac gaccgcatcc gtggcgtgcc
     1261 atgtttcgac cgcgtgatgg agagcctcga catcgcccgt gctctcggcg aacatcccga
     1321 catcctcttt acggcaacgg aggagacgtt cgaacagatc gcccctcttg ccgccatcgc
     1381 tcaggcgtac aataccatgc tcattgtgaa tccagagttt gcgtgggcag ggaacaaagg
     1441 tgtcggagaa cgggaactgg cctgccttga acgtttcgcc cgcgagcgga acgtctacgt
     1501 gaaccgggcg tttcatcgtc tcattcgagc tggcggcaat caacgcgcgc accccgtctg
     1561 tcacgcggta gaagccgtcg tcgtcatctc accggacgat cacctgctcc taccctgctt
     1621 ccatcggcag agagaacgca taccgataaa cggccagttg gaaaaagtgc gggcaggttc
     1681 agtagtgaag gcgtacaaag gcgaggatgg caggcagccg tactgccagg gttgcacgat
     1741 caattgctac atggatccat cgtttcagta cgcatgcaat gcctatcttt tcctcagcct
     1801 ggcggcaaag gcaaaatacg cgtggagcaa gtacgtcctt ctggcctgaa ccctccagca
     1861 ccggctcgtg gcacgccatt gcccccgtgc cacctgttcc cccgctctcc ttctgacata
     1921 tccgccctat ttgccctatt cgacctgttc gctccaatgc cttcatgcca cattccctag
     1981 cacacgcctc ctcaccgccc cttcccgtac gcctccagac cgtactccct cccgaagcgg
     2041 aacaactcca cgatgttctc tgtcggaact tcggggccga ccgaactgga aggcgcgaga
     2101 aaaaacctgc caccacgccg cgcgccgatg tcaatgcacc gtttgacatc tttccttacg
     2161 tcctcgacac ttccgtgagg cagcgtcgag gtcacgc
//
LOCUS       DAOSOO010000350         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_49871_length_2192_cov_7.655592, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000350 DAOSOO010000000
VERSION     DAOSOO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49871_length_2192_cov_7.655592"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1778)
                     /locus_tag="PLT86_14765"
     CDS             complement(63..1778)
                     /locus_tag="PLT86_14765"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOT37922.1"
                     /translation="MYTRPIGHTQHVVTDQGPAVHRVHLIGPHPILNAFLSRMEFARI
                     VRSCLGTPHQGILDHARTLSVFIQNIILSPAPLYRIAEWAHPIDPAALGFTAVEKRSL
                     NDDRVARSLDALVSLRARTLFFQLAVHIIKQFELDTRRIHHDTTTVTFTGLYQGSHCE
                     PRITRGMNKDHRPDLKQLVFGLNVTADGAVPISHEVYSGNRTDDTIHRSNLDRLRQVL
                     GRTDFIYVADSKLATRTNLGYVSKYGGKFVTVMPRSRAEDKRFRKLLRDVAPVRWRKL
                     LEIENHRRKSDPPDVYESTADGPEVTREGYRIVWLRSSQKRELDAFGRETSLEKAERD
                     LFAINTRLNRGKLRQRAAIQKKIKEILAKHNCLRFLHVHIASRTHIETRHLRRGRPRT
                     DDPVKRIHIRVYHLQVRRHKEALRAEARTDGVFPLITNLEKAEASKAEVLSIYKYQPY
                     VEKRHALFKTELEVEVAPVYIKKPLRAVGLIHAVFLAMTLDALIERTVRLAMIREGIE
                     ALPILPEGRLTRTPTTARILEMFSNVCWYEFARGDETVTFPIQLSALQKELLRLLGMN
                     ASTYA"
     gene            2057..2182
                     /locus_tag="PLT86_14770"
     CDS             2057..2182
                     /locus_tag="PLT86_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37923.1"
                     /translation="MLRAGEGYREFRKDMKQIRAIDLKEKQILMALAEERAIHYE"
BASE COUNT          440 a          608 c          676 g          468 t
ORIGIN      
        1 cctacattcc gcagctcttg acggcgaccc gggggaattt ccgagcatct cgacgcggcc
       61 cttcaggcat aagttgaggc gttcatgccg aggagccgaa gcaattcctt ctgcagggca
      121 gagagctgga tagggaatgt gaccgtttca tccccccgcg cgaattcgta ccaacagacg
      181 ttactgaaca tctcgagaat ccgagccgtg gtcggcgttc tggtcagccg tccttcgggg
      241 aggatcggga gcgcctcgat gccctcgcga atcatggcga gccgaacggt ccgctcgatc
      301 aacgcgtcaa gcgtcattgc gaggaacacc gcatggatca accctacggc acgtagtggc
      361 ttcttgatgt acacgggagc gacctcaacc tcaagctcgg tcttgaacag tgcatgacgt
      421 ttctcgacgt agggttggta cttgtagatt gacagaacct cggccttcga agcctccgcc
      481 ttctccaagt tggtgatcag cgggaagact ccgtcagttc gagcctcggc tcgaagtgcc
      541 tccttgtgtc gtctgacctg caggtggtag acacgaatgt gaattcgttt caccggatcg
      601 tctgtacgcg gccgcccgcg gcgcaagtgc ctggtctcaa tgtgcgttct ggaggcgatg
      661 tgtacgtgga gaaaccgcag gcagttgtgc ttcgcaagga tctccttgat cttcttttga
      721 atagcggcac gctgacgtaa cttgccacgg ttgagccgtg tgttgatggc gaagagatcg
      781 cgctcggctt tctccagtga cgtttctctc ccgaaggcgt caagctccct cttctgggag
      841 cttcggagcc agacgatgcg gtagccttcg cgggttactt ccggcccgtc tgctgtcgat
      901 tcgtacacgt cgggcggatc gctcttgcgc cggtggtttt cgatctccag aagctttcgc
      961 cagcgcactg gggccacatc tcggagcaac ttacgaaagc gtttgtcttc ggcgcggcta
     1021 cgcggcatga ccgtgacgaa cttcccgccg tacttggaga cgtagccaag gttcgtccgt
     1081 gttgccagct tgctatccgc gacatagatg aagtcggtcc ggcctaggac ttgccgcaac
     1141 cgatccaggt tgcttcggtg gatggtgtcg tccgtccggt taccgctgta cacctcatgg
     1201 gagatcggaa cagcgccgtc cgcggtgacg ttgagtccaa agaccagctg tttcaggtct
     1261 ggccggtgat ctttgttcat ccctcgagtg attcggggct cgcagtgcga tccttggtaa
     1321 aggccagtga aggtcaccgt cgtggtgtcg tggtggatac gtctcgtgtc cagctcgaac
     1381 tgcttgatga tgtgaacggc caattggaag aacagcgtcc gcgcccgcag ggagaccagc
     1441 gcatccaggc tgcgggctac gcgatcgtcg ttcaacgacc ttttctctac ggcggtgaat
     1501 cccagtgccg ccggatcaat gggatgggcc cactccgcaa tccggtacag tggagcgggc
     1561 gagaggatga tgttctggat gaagacggaa agggtccggg cgtgatcgag aatcccctgg
     1621 tgcggggtgc ccaagcacga gcgtacgata cgcgcgaact ccatgcggct gagaaacgcg
     1681 ttgaggatag ggtgagggcc gatgaggtgt acccggtgga cggctgggcc ttggtctgta
     1741 accacgtgct gggtatggcc gattggtctg gtatacataa cattaatagt gtatactata
     1801 ccccatgacg cagtcaagaa caaaattgct cgcgatgctc aggcggctct accgggagcg
     1861 tcagcgtctg gttcgccgcc tcacaggagg acgcgaactc gcgattggca ccgtcacgac
     1921 aacgaatcgc aagtgtggta acccgagttg ccgctgcgcc aaaggcttgg gccatctcca
     1981 aaccgtgttt ctcttcaagg acaagaagga gggccgtcgt cggtgcaagc tcatccggcg
     2041 agcggacgag gcccgaatgc ttcgcgcagg cgaaggctac cgggaattcc ggaaggacat
     2101 gaagcagatc cgcgccatag atctcaagga aaagcagatc ctaatggcgc tcgcagagga
     2161 gagagccatc cactacgaat agcataacat ag
//
LOCUS       DAOSOO010000351         2186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_50055_length_2186_cov_5.577663, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000351 DAOSOO010000000
VERSION     DAOSOO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50055_length_2186_cov_5.577663"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLT86_14775"
     CDS             <1..188
                     /locus_tag="PLT86_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT37924.1"
                     /translation="SRSVNLHPLLIFLAVVFGGVYGGLLGMFLAVPLTGAFVVTARTI
                     REGLRPPVYPYDVIAEL"
     gene            complement(206..1093)
                     /locus_tag="PLT86_14780"
     CDS             complement(206..1093)
                     /locus_tag="PLT86_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT37925.1"
                     /translation="MDFTAPPVARDILDARNNASDRTLNNGPAAKKERVGTMMTAVII
                     DDEQPARDELRYLLRKHADIEVIGETASGTAAVELIGERAPDLVFLDIQMPGMTGFQV
                     VQELMDREEELPAIVFVTAYDEYAVRAFEVSAVDYLLKPVEEERLAQALDKVRRQAAA
                     TALSREEISRLVRQMEEGERTATLPHRISVRKGDRYVLVDPNDVTHAYVVDGVVCLCT
                     SKLNGMTTHKTLEELEKDLDGRIFWRVHRSYIVNINHVAEILPLPTGSYRLRLDDEKK
                     TTVPLSRAQAKLLRKVVKW"
     gene            1171..1740
                     /locus_tag="PLT86_14785"
     CDS             1171..1740
                     /locus_tag="PLT86_14785"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT37926.1"
                     /translation="MRAVVLNGLREGDVGQAISDEVRSRLEHAGWTTEEFVLRDRNVA
                     PCVEDFGCWIRKPGQCLIKDDAQEITRAFLTCDLVVFLTPVTFGGYSSELKKVLDRSI
                     CFALPYFEGVGGETHHPTRYDRIPAMFYVGTETAPDEQAESVFRDLVRRNALNMRPRA
                     YRVSFVRAEKDAARVAEEVLALANEEYGS"
     gene            1737..>2186
                     /locus_tag="PLT86_14790"
     CDS             1737..>2186
                     /locus_tag="PLT86_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H dehydrogenase"
                     /protein_id="HOT37927.1"
                     /translation="MTGKPRILFAVGSQKPAESNSRSLADYLAEQLRKRGVPSKQIHL
                     LPLVRGEERWNELFAAVDEADFLFLVFPLFVDSQPWSVVRFMELVGAHRRSSNAGPMR
                     VAALVNSGFPEARHNEPALAICRQFAVESNMSWAGGLALGSGPVIGGK"
BASE COUNT          373 a          671 c          648 g          494 t
ORIGIN      
        1 tgtcacgctc ggtaaacctg caccctctcc tcatctttct cgcggtggta tttggtggcg
       61 tgtacggcgg cctcctcggg atgtttctcg ccgtccccct gaccggcgcg ttcgtggtta
      121 ctgctcgcac gatccgggaa ggccttcgtc ctccggtcta cccgtacgat gtgatcgctg
      181 agctgtagcc gccgcttcgc gcttctcacc acttcaccac tttcctcaac agcttcgcct
      241 gcgccctgct gagaggaaca gtggtttttt tctcgtcgtc gaggcggagg cggtaggatc
      301 ccgtcggaag cgggagtatc tccgcgacat gattgatgtt gacgatgtag ctccggtgga
      361 cgcgccagaa gattctcccg tcaaggtcct tttccagctc ttcgagcgtt ttgtgcgtag
      421 tcatcccgtt caacttcgat gtgcacagac atacaacacc gtccacgaca tacgcgtgcg
      481 tcacgtcgtt cgggtccacc agtacgtacc ggtcgccttt tcgtaccgag atccggtgcg
      541 ggagtgtggc ggttcgttca ccctcttcca tctgccggac cagccgggaa atttcctccc
      601 gtgagagcgc cgtcgccgcg gcctgcctgc gcactttgtc cagcgcctgt gcgagccgct
      661 cctcttccac cggcttcagg aggtaatcca ctgctgaaac ttcaaacgcg cgcaccgcgt
      721 actcgtcgta tgcggttaca aacacgattg ccgggagttc ttcttcccgg tccatcagtt
      781 cctgcaccac ctgaaatcct gtcattcccg gcatctgtat gtccagaaag acaagatcgg
      841 gcgctctctc gccgatcaat tccaccgccg ccgttccgct ggcggtctcc ccgatcactt
      901 cgatatcggc gtgcttcctc aggagatagc ggagttcatc acgtgcgggc tgttcatcat
      961 ctatgatcac tgcagtcatc attgtcccaa ccctttcctt tttcgctgcc ggcccgttgt
     1021 tcagagtccg gtcagacgcg ttgttccgtg cgtctaaaat atcgcgcgca accggcggcg
     1081 ctgtgaaatc catcctgatg gcgcccgcat tacacggcga gtatctttac aggaggacat
     1141 cgtcttgcag gatgcgtgca cgggggtagc atgagagctg tggttctgaa cggcctgcgc
     1201 gaaggggacg tcgggcaggc gatctcagat gaggtacgtt cccgcctcga gcacgcgggg
     1261 tggacaacgg aggagttcgt ccttcgcgac aggaacgttg ccccctgcgt ggaggacttc
     1321 ggttgctgga ttcggaaacc cggccagtgc ctcatcaagg atgacgctca ggagataacc
     1381 cgcgcgttcc tgacctgtga tctggtggtc ttccttactc ccgtcacgtt cgggggctac
     1441 tcgtccgaac tcaagaaagt gcttgaccgg agcatctgtt tcgcgctccc gtatttcgag
     1501 ggagtaggcg gcgaaacgca ccatccgaca cggtacgacc gcattccggc catgttctac
     1561 gtcggaacgg agacggcccc ggacgaacag gcggagagtg ttttccgcga cctggtccgg
     1621 cgcaacgcgc tcaacatgcg tccgcgggcg taccgggtct ctttcgtgag agccgagaaa
     1681 gatgccgccc gcgtcgcgga agaggtgctg gctctcgcga acgaggagta tggatcgtga
     1741 cggggaagcc gaggattctc tttgcagtgg ggagccagaa gcccgcggaa agcaactcac
     1801 ggtctctggc tgattacctt gcggagcagc tccgcaagcg cggcgtaccc tcgaagcaga
     1861 tacacctatt gcccctcgta cgcggcgaag agaggtggaa tgagctcttt gcagccgtgg
     1921 acgaggctga tttcctgttt cttgtgtttc cgctgttcgt ggatagccag ccgtggtcgg
     1981 ttgtgcgttt catggaactt gtcggtgccc accggcgatc gtcgaacgcc ggcccgatga
     2041 gggtggctgc tctggtcaac agcggctttc ccgaggcacg ccacaacgag cccgcacttg
     2101 ccatctgccg ccagttcgcc gtagaatcca atatgtcgtg ggccggcgga ctggctcttg
     2161 ggagcggccc cgtgatcggt gggaag
//
LOCUS       DAOSOO010000352         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_50123_length_2184_cov_4.123532, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000352 DAOSOO010000000
VERSION     DAOSOO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50123_length_2184_cov_4.123532"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLT86_14795"
     CDS             complement(<1..972)
                     /locus_tag="PLT86_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37928.1"
                     /translation="MPETVNVIVPVKRMSPKGEHCFFGYYDIPAADEAGRHLCHRVQF
                     RDRLPRRGDVAELGWLALPDNADAEPDGIFHPIAETEAWNFQQSSMAQWLASEPDTCL
                     FNTFEHEAYGSRIHDIRTGRGRLLPLPVANVSRDGTKALCINMSRLFDFRPGYGYEEL
                     PDPFADVAQPRDDGVRLMDIRTGNNRLILSLAEIVEFLEQSGERIGGKKVLVNHITFN
                     PSATRFLLLLRTFPEPGKRWETFLVTSDLEGRNLRRHNVWGIASHYHWRDDDGMLFYM
                     NVSGPNTLELALVRDSTRSIECIDPAYFRCDGHCSYSPDLRWILYDSY"
     gene            complement(987..2042)
                     /locus_tag="PLT86_14800"
     CDS             complement(987..2042)
                     /locus_tag="PLT86_14800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT37929.1"
                     /translation="MIQKFTVSRDHTIYEAWPDVALTPSGRLVCVFSECTHHGDRSYT
                     RIVLTDSTDRGRTWTPKRPLTEPTRGLPVFWNCARITYLRDGRLVVVVDKLFKPEGSA
                     APEDCRNYLFFSEDEGATWSLPVETPALGIVPDRLLELASGRWILACHFHDKPFGYLV
                     QRLWYSDDRGASWTGPVIVGKKEGLNLCEASILPVRDSLVAFHRENSRQGWDCFKSIS
                     RDDGETWSPVVAFPLPGCHRPVAGMLHDGRILITYRFMQGGKGWVGWWTQNFFAALTD
                     KESALAETRNEAHTRIMPVDFDRSPVSDTGYSGWVQFEDGEIYIVNYIVDDAPKAQIR
                     GYSLHPSDFLFDEPSQP"
BASE COUNT          426 a          668 c          661 g          429 t
ORIGIN      
        1 gtagctgtcg tacagaatcc agcgcagatc gggcgaatag gagcagtggc cgtcgcagcg
       61 gaaatacgca gggtcaatgc actcgatgct gcgggtggaa tcgcgcacca gagccagctc
      121 cagagtgttc ggtccgctga cgttcatgta aaacaacatg ccgtcgtcgt cgcgccagtg
      181 gtagtgcgat gcaatgcccc atacgttgtg acgacggagg ttccggccct ccagatcgga
      241 tgtgacgaga aacgtctccc accgtttccc cggctccggg aacgtgcgca gcagaagcag
      301 gaatcgtgtt gccgacggat tgaacgtgat gtggttgaca agcaccttct tgccgccgat
      361 ccgctcccct gattgttcga gaaactcgac gatttccgcc agtgacagaa tcagccgatt
      421 gttccctgtt cgaatgtcca tgaggcggac accatcatcc cgcggctgtg caacgtccgc
      481 gaacggatcg ggcagttctt cgtagccgta ccccggccgg aaatcgaaca ggcgggacat
      541 gttgatgcag agcgccttgg tgccgtcccg ggagacgttc gccacgggca acggcagcag
      601 tcgaccccgt cccgttcgga tgtcgtgaat gcgcgagcca tatgcctcgt gctcaaacgt
      661 gttgaacagg caggtatccg gctctgacgc gagccactgc gccatggagc tctgctgaaa
      721 gttccacgcc tcggtctccg cgatgggatg gaaaatcccg tcaggttctg cgtctgcatt
      781 atcgggcagt gccagccatc cgagttccgc cacgtcgccg cgccgaggca ggcggtcgcg
      841 gaattgcacg cggtggcaga ggtggcgccc ggcctcgtcc gcggccggga tgtcgtaata
      901 cccgaaaaaa caatgctccc ctttcggcga catgcgtttg accgggacga tcacattcac
      961 ggtttccggc atggtgtcct cactgttcag ggttgtgacg gttcgtcaaa aaggaaatcg
     1021 gacgggtgga gcgagtaccc gcgaatctgc gccttcggtg cgtcgtccac gatgtagttc
     1081 acgatgtaga tttcgccgtc ctcgaactgc acccagccgg agtagcctgt gtcgctcacc
     1141 ggcgagcggt cgaaatcaac gggcataatg cgtgtgtgcg cctcgttccg tgtctcggca
     1201 agcgctgatt ctttgtccgt cagcgcggcg aaaaagttct gcgtccacca gcccacccat
     1261 cccttgccac cctgcatgaa ccggtacgtt atcagaatgc gaccatcatg gagcatgcct
     1321 gccacggggc ggtggcaacc cggcagggga aatgccacta ccggcgacca ggtctccccg
     1381 tcatcgcgtg agatgctctt gaaacaatcc cacccttgcc gggaattttc gcggtgaaat
     1441 gccaccagtg aatcgcgcac cggaaggatc gaggcttcgc acaggttcag gccctccttc
     1501 tttcccacga ttaccggtcc cgtccacgag gcgcccctgt cgtcggaata ccagagccgc
     1561 tgcacgaggt aaccgaacgg cttgtcgtgg aaatggcagg cgagaatcca gcgcccgctt
     1621 gccagctcga gcagcctgtc cgggacaata cccagcgcgg gtgtctcaac aggcagcgac
     1681 cacgtggccc cctcatcctc gctgaaaaac aggtagttcc ggcaatcctc cggcgcagcg
     1741 ctgccttcag gcttgaaaag cttgtccacc accaccacca gccggccgtc gcgcaggtac
     1801 gtgattcggg cgcagttcca gaacaccggc aggccgcggg tgggctcggt gagcggccgt
     1861 ttaggcgtcc acgtgcgccc tcggtcggtg gagtcggtca gcacgatgcg cgtgtaggag
     1921 cggtcgccgt ggtgcgtgca ctcggaaaac acgcatacca gcctcccgga cggtgtcagc
     1981 gctacgtccg gccacgcctc gtaaatcgtg tggtcgcggc tgacggtgaa tttctggatc
     2041 atggtgtctc cttgtttgga tccggtgcca tcgtgaagga agctacttcc ggaacacatt
     2101 ctgccgaaag tcagtccggg tgccgtatca agctgaggca aaacgtcgtc ttcgtgtcaa
     2161 gggttttcgc gaaaatagtt caat
//
LOCUS       DAOSOO010000353         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_50718_length_2166_cov_3.575557, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000353 DAOSOO010000000
VERSION     DAOSOO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50718_length_2166_cov_3.575557"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..512)
                     /locus_tag="PLT86_14805"
     CDS             complement(15..512)
                     /locus_tag="PLT86_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37930.1"
                     /translation="MAVRSASRSSAVTARSVIGGSLLVVLVSIVTPYSEYRLHSVELF
                     QGQLPLGALATLVVIVLPLQWLLARFFPAWRLRESEILFMFSMGFAGLMVYHIGMMGL
                     FLSMVSSPEYFASPENQYARYLLPYLPAWAIVPNSNSAMTWFYTGLPAGAAIPWRVWV
                     GPLFW"
     gene            769..1704
                     /locus_tag="PLT86_14810"
     CDS             769..1704
                     /locus_tag="PLT86_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT37931.1"
                     /translation="MAQIRVLVCDDEPDARFLLRSRLVSLGYDVLEASDGDECVKIVA
                     SDPVDIIILDVSMPRVDGFSACSELRRNPRTRHIPIVFLTALRTSRDDRLKGLRLGAD
                     EYLVKDVDPEELAARLEAVLRRTVGAVETNPLTHLPGNAVVTEEIESRIRKREPFAVA
                     WADLDNFSAFNDRYGFARGDKLIVETATILLESLKLFGSEEDFLGHLGGDDFVVVSTV
                     ERSAMMAEHAVKLGRERLPLLYDLDDRSRGYVRTLDRNGKQLQYPITNISVAIVQSRG
                     QRFQNILQVADAANEMKRRAKMNPGSGVVIDRRVH"
     gene            1840..>2166
                     /locus_tag="PLT86_14815"
     CDS             1840..>2166
                     /locus_tag="PLT86_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37932.1"
                     /translation="MVVSPHPDDSVLGAGGLICRLTDRGSWARKFGDSGQDQPAVYTL
                     VMTSGSRGIDDEYLRTAELERIRSRDPALADYVLNKPRQGNPDAELEKRLVEIRTEIR
                     RAESQAE"
BASE COUNT          486 a          603 c          674 g          403 t
ORIGIN      
        1 aagaaaaacg accactacca gaacaacggg ccgacccaca ctctccacgg gatggccgcc
       61 ccggccggga gcccggtgta aaaccaggtc atggcggagt tggaattcgg aacaatcgcc
      121 cacgccggga ggtacggcag aaggtatctc gcgtactggt tttccggact cgcgaagtac
      181 tcgggggatg agaccatgct gaggaacagg cccatcatgc cgatgtgata caccatcaat
      241 cccgcgaaac ccatcgagaa catgaacagg atctcggatt cgcgaagacg ccacgcgggg
      301 aagaaacgcg cgagaagcca ttgaagcggg agaacgatca ccacaagcgt tgccagcgcg
      361 cccagcggaa gctgtccctg gaacagctcg acggaatgaa ggcggtattc ggaatacggt
      421 gtgacgatgc tgacgagaac aacaagcagg gacccgccga tcacggatcg ggcggtaacc
      481 gcggaggagc gagacgcgga acgaacggcc atggatcaac ccgccgaggg ttggtgccgc
      541 ggggccatcg cccatgcgga tcgatggcac cgtactgccc gaagcacgcg aggaaaaaga
      601 tcagaaaggg ccaaaacagg ggcaacccgg ccttacggtg tgttgacccc gcgcggcaag
      661 acgctatatt ctgtaaaatc catcacattt ttccgcgcct ccagatgatg gattgcgcct
      721 gtgtggtcat tagtcgccac acatcaccac gcccacccgg gagacttcat ggcacagatc
      781 agagttctcg tatgcgacga tgaaccagat gcacgcttcc tgttgcgttc gcggcttgtg
      841 tctctgggtt acgatgttct cgaagcgtcg gacggcgacg agtgtgtcaa aatcgtggca
      901 agtgatccgg tcgacattat catacttgac gtctccatgc cgcgcgtgga cgggttttca
      961 gcctgctctg aactgcgtcg caatccgaga actcgacata ttccgatcgt gtttctcacc
     1021 gcgctgcgaa ccagccgcga cgaccgcttg aaaggcctcc gcctgggcgc cgacgagtac
     1081 ctcgtgaagg acgtggaccc tgaagaactg gcggctcgtt tggaagcggt attgcggcgt
     1141 accgttggcg cggtcgagac gaatccgctt acccatctgc ccggcaacgc ggtggtcacc
     1201 gaggaaattg aatcgcgtat ccggaaacgg gagccatttg cggttgcgtg ggcggatctc
     1261 gacaacttca gcgcgttcaa tgatcggtac gggttcgcgc gtggcgacaa attgatcgtg
     1321 gaaacggcta caatcctcct ggaaagcctg aagctgttcg ggagcgaaga ggattttctc
     1381 gggcacctcg gcggcgacga cttcgtggtg gtatcgacgg tagagcgctc cgcaatgatg
     1441 gcagagcacg cagtgaaact cggccgcgag aggcttccgc ttctctatga tctggatgat
     1501 cgctccagag ggtatgtgcg aaccctggac cgtaacggga agcaactcca gtatccaatc
     1561 accaatatct cggtcgcgat cgttcaatca cgggggcaga gattccagaa cattctgcaa
     1621 gtcgccgatg ctgcgaacga gatgaaacgg cgcgcgaaaa tgaatcccgg ttccggggtc
     1681 gtgatagatc gccgcgtcca ttgacttccg ggtggcagcc aggtttgcca ttccgtacat
     1741 tagtggacgt tgatccggtc ctttgtcagc gctgtcttct ggtcgggggg cgccttgata
     1801 cacaccgatt tctacgaatt gttcaggcgg acgctcgtaa tggttgtctc accgcacccg
     1861 gacgattccg tgctgggagc gggtgggctt atctgccggc tcacagaccg tggttcctgg
     1921 gcgcgaaaat tcggcgactc tggacaagac cagcccgccg tgtacacgct cgttatgact
     1981 tccgggagca ggggaattga tgacgagtac ctgcggacgg cggagttgga gcgcatacgc
     2041 tcccgggatc cggctctcgc ggattatgtg ctgaacaagc cacgccaagg gaatcccgac
     2101 gcggaattag agaagaggct ggtggaaatc cggaccgaga ttcgtcgggc ggagagccag
     2161 gccgaa
//
LOCUS       DAOSOO010000354         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_50756_length_2165_cov_4.095735, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000354 DAOSOO010000000
VERSION     DAOSOO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50756_length_2165_cov_4.095735"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="PLT86_14820"
     CDS             complement(<1..1986)
                     /locus_tag="PLT86_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37933.1"
                     /translation="MIADIATDLVSLQASFSSASDEIKSLIIRISSEIDFVNKLSELH
                     AEEAEAWRILARKALDVVQGSVAGGKMDSIRGAVAEAEAILVPVANTAKSYTIWCVGH
                     GHIDMNWMWSWPETVAVTNDTFSTVDRLMSEYPDFCYSQSQTSVYEIARKYNPTLFQR
                     IKERVAEGRWEPTACMWVEGDKNLAGGESLCRHLLYTRQFMEEHFGLKPADVLIDWEP
                     DTFGHAHTIPHFLARAGVRRYYFCRGGADPKPSVFWWEGPDGSRVLCYREITWYNWEV
                     RPELTNHLVAFAKETGLRAWMNVYGVGDHGGGPTREHIEWAHRMDSWPVFPSIKLSTT
                     KPFYELLEENSAKWDVLKRELNFEFTGCYTTQTSIKRNNRFAEQLLYQAEAASSLAWL
                     EAAQEYPEPTLRDGWINTLFSQFHDILPGSCVRPSREYNSGQYQETAAGAGMALTNSL
                     RAVASEIDTACFGGSGAIGASANTIGGGAGFESGMGAVSAYDGGSSDVRPFVLCNLNG
                     WERSEVLIATVWNSGWKDIVVQDDAGNRFLGQIVEKGGYWGHEFVKVAFPALKIPALG
                     YRTFALAQGHAPVAACALAHVEHGAENEYFRLEMDWNTGGIKSLVEKRSGVDLAIPNR
                     PMGLLEYAVERHHGMSAWVIGDIQTSRLLGEITSLEEK"
BASE COUNT          434 a          687 c          572 g          472 t
ORIGIN      
        1 tttttcttcg agcgaggtga tctcgcccag aagcctcgat gtctgaatgt cgccgatgac
       61 ccaggcggac atcccatgat gtcgttcgac cgcatactca agaagcccca ttggacgatt
      121 cggaattgcc aggtcgacgc cgctcctttt ctccaccaga ctctttatgc caccggtatt
      181 ccagtccatc tcgagccgga aatactcgtt ttctgcgccg tgctccacgt gcgccagcgc
      241 acacgccgcc acgggggcat ggccttgagc gagagcgaac gttctgtacc ccaacgctgg
      301 tattttgagc gcgggaaatg ccaccttgac aaattcgtgg ccccagtacc cgcctttttc
      361 tacgatctgt ccgagaaaac ggtttcccgc gtcgtcctga accacaatat ccttccaacc
      421 ggaattccac acagtggcga tcaatacctc gctgcgttcc cacccgttga ggttgcagag
      481 cacgaacggc cttacgtcgc ttgagccgcc gtcgtatgcc gagacggcac ccatccctga
      541 ttcgaacccc gcgccaccac cgattgtgtt tgctgacgcg ccgatcgctc cgctcccgcc
      601 gaagcatgcc gtatcaatct ccgaagcgac tgcgcgaagg ctgttcgtca gcgccatccc
      661 ggcgcctgcg gcagtttcct ggtactggcc tgagttgtac tcccgggaag gccgcacgca
      721 cgaacccgga agaatgtcgt gaaactggga gaaaagcgtg ttgatccagc catcccgcaa
      781 agtcggttct ggatactctt gtgcggcttc cagccacgcg agcgaggacg cggcctccgc
      841 ttggtaaagc agttgttccg caaatcggtt gttacgtttg atgctcgtct gggtggtgta
      901 gcagccggtg aactcgaagt tcagttctcg tttgagcacg tcccatttcg cggaattctc
      961 ttccagcagt tcgtaaaagg gcttcgtcgt cgaaagcttg atggacggaa acactggcca
     1021 cgaatccatc cggtgtgccc actcgatgtg ttcgcgcgtg gggcccccac cgtggtcccc
     1081 caccccgtac acattcatcc aggcccgaag cccggtctcc ttcgcgaacg cgaccagatg
     1141 gttcgtcagt tccggcctga cttcccagtt gtaccacgtg atttcgcggt agcaaagaac
     1201 ccgggaacca tcgggcccct cccaccagaa cacggacggc ttcgggtcgg ctcctccccg
     1261 gcagaagtaa tacctccgaa cgcctgcccg ggcgaggaaa tgcgggattg tgtgtgcgtg
     1321 accgaacgta tcgggttccc agtcaatgag gacgtccgcg ggcttgaggc cgaagtgctc
     1381 ttccatgaac tgacgggtat acaggagatg acggcagagg ctctcccctc ccgcgaggtt
     1441 cttatcgcct tccacccaca tgcaggcagt cggttcccac ctgccctcgg ctacacgctc
     1501 cttgatccgc tgaaacagcg ttggattgta cttgcgggca atctcgtata cacttgtctg
     1561 gctctgcgaa taacagaagt cggggtactc cgacatcaac cggtccaccg tgctgaatgt
     1621 gtcgttcgtc actgccacgg tttccggcca ggaccacatc cagttcatgt caatgtgacc
     1681 gtgaccaacg caccagatgg tgtagctttt cgcggtgttc gccaccggca ccaggatcgc
     1741 ctccgcttcg gccaccgcac cgcggatgga gtccatcttt ccacccgcaa cgctgccctg
     1801 aacgacatca agagcttttc gcgcgaggat tctccatgcc tcggcttctt ctgcatggag
     1861 ttcactcagt ttgttcacaa aatcgatttc cgacgatatg cggattatca gcgacttaat
     1921 ctcgtctgaa gcggaagaaa acgaagcctg aagcgaaacc aggtcggtgg cgatgtctgc
     1981 gatcattgaa agatccttcg caaaggttgg cgtgaagggt ggccgtttga gtatctcaaa
     2041 tggtacctaa cagcggcatg aagggaaaac cctctcatac cgcatccctt gacccggccg
     2101 ccaacccgtc ctaccatgac gtcgtttcgt gccgtttcac cccgcaacag aaagaccccg
     2161 ccgtg
//
LOCUS       DAOSOO010000355         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_51771_length_2134_cov_4.873016, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000355 DAOSOO010000000
VERSION     DAOSOO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51771_length_2134_cov_4.873016"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLT86_14825"
     CDS             complement(<1..393)
                     /locus_tag="PLT86_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37934.1"
                     /translation="MKQAGIRHVFFALCVGLALPALASAAFAGASADSIAGKVRVVPN
                     PWCASDQAQHYAKRTGNSIFDVKNGYSRVLFVNLPNPCTIRIYTVDGDLVRTIENTAG
                     SSTTWDLITESDQAVTTGIYVWTVDSAVG"
     gene            complement(727..>2134)
                     /locus_tag="PLT86_14830"
     CDS             complement(727..>2134)
                     /locus_tag="PLT86_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOT37935.1"
                     /translation="DMRLVWVRDGDQDPSKYDAVATGLPSQDDYGDPRPITGELLSAR
                     HMGLMILHWDKAVSDRRDDIAQPMTCGWGEGGGSLDQNWRTARDGHEVKYRQMRYGYQ
                     YGDIYYEGDITDTPARGAHPFGGSWIKASNDLPDRETWWPGSIVGVTDEVTQVEQQLG
                     MGPTDIPPNDTLNALYVEAVAGHSLPYAAWVGREWAAGRLTDAQKDAIVDQCQDSLFT
                     VMRRAKAIYESATFPDGHGGTRFASTRDELHVAYAAAIAAGKLSLSPPAPASFTVVSG
                     KSKVSLYWTLNTTTGSNIAGWRVYRAVADFRGDSAWTKIYDGPPSVLGYVDKGVTPGF
                     SYYYYLTTYDANGLESTMHTRTNAAAIPLTGDDTGVLDADRALRFALEQNAPNPFNPT
                     TTIRFSVAASGPTRLDIYSTNGQLIRTLVNGSVAAGVNSVQWDGKDAMGRPVASGTYL
                     YRLTNGTNTSVRRMVLVR"
BASE COUNT          380 a          709 c          632 g          413 t
ORIGIN      
        1 cccgaccgcg ctgtccacag tccagacgta gatacccgtc gtcactgcct gatcgctctc
       61 ggtgatcagg tcccatgtcg tgctggagcc cgcggtgttt tcaatcgttc tgacgaggtc
      121 tccgtctacc gtgtaaatcc gtatcgtgca cgggttcggc agattcacga acagcacgcg
      181 ggaatagccg ttcttcacat caaagatcga attcccggtt cgcttcgcgt aatgctgcgc
      241 ctggtccgac gcgcaccagg gattcggcac cacacgaacc ttgcccgcaa tgctgtccgc
      301 acttgctccc gcgaacgccg ccgaggcaag ggcgggcaaa gccagcccca cacacagcgc
      361 gaagaacaca tgcctgatcc cggcttgctt cattgcggtt cctcctgatt gcagttgccc
      421 gtcaatacca tactgcttct atcgattacc ctgaaatctg catctccccg gcccacctcg
      481 gcatcggcct ttccccgcgc ggcggaaacc gacctgagcg aaacgaacac gcgtgcgctc
      541 gccgtattcc cttcgctcaa cccggttccg tcactccggt gatcggggtg cgcgccccga
      601 aagacgcgca ccctttgctc atctcatgac cttacgcttc tcaattctac gcaaacctgc
      661 cgttcgcctc agcagcgggg gcgcatcgcg ccccccccaa ggtacgccaa gcgaacgcca
      721 gttcgcctag cgtaccaaga ccatgcgccg gacgctggtg ttcgttccgt tcgtcaggcg
      781 gtagagatac gtaccgctgg caaccgggcg gcccatcgca tccttgccat cccactgaac
      841 gctgttcaca cccgccgcga cagaaccgtt cacgagcgtg cggataagct gcccgttcgt
      901 gctgtagatg tcaagacggg tcggaccact cgccgcaacg gagaagcgaa tggtcgtggt
      961 ggggttgaag gggttcggcg cgttctgctc aagcgcgaac cgcagagcgc gatcggcatc
     1021 cagaacaccc gtgtcgtcgc cggtgagcgg gatcgccgcg gcgttggtgc gcgtgtgcat
     1081 cgtggactca agaccgttgg cgtcgtacgt cgtgagataa tagtagtacg agaatcccgg
     1141 tgtcacgccc ttgtcaacgt agccgagcac actgggcggc ccgtcataga ttttcgtcca
     1201 ggccgagtcg ccacggaagt ccgccaccgc tctgtacacc cgccagccgg cgatgttcga
     1261 accggtggtg gtgttgagcg tccagtacag actcaccttg gatttgcccg acacgaccgt
     1321 gaaggatgcg ggcgccggag gcgacaggga gagtttcccc gcagcgatgg cagccgcgta
     1381 tgcaacgtgc aactcgtctc gtgtggacgc gaaccgcgtc ccgccatgcc cgtcggggaa
     1441 ggtggccgat tcgtagatcg ccttcgcccg gcgcatcacg gtgaacagcg agtcctggca
     1501 ctgatccacg atggcgtctt tttgcgcgtc ggtaagccga ccggcagccc actcacggcc
     1561 aacccatgcc gcatacggca ggctgtgccc ggcaaccgct tccacgtaca gcgcgttcag
     1621 cgtgtcgttt ggcggaatgt cggtcgggcc catgccaagc tgctgctcaa cctgtgtcac
     1681 ttcgtcggtt acgccgacga tggagcccgg ccaccatgtc tcacggtcgg gcagatcgtt
     1741 gctggctttg atccacgaac caccgaacgg atgggcgcca cgcgccggag tgtcggtgat
     1801 gtcgccttcg tagtagatgt caccgtactg atagccatag cgcatctggc ggtacttcac
     1861 ttcatggcca tcgcgcgcgg tgcgccagtt ctggtcaagg cttccgccgc cttcgcccca
     1921 gccgcaggtc atcggctgtg cgatgtcgtc acggcggtcg ctcacggcct tatcccagtg
     1981 gagaatcatg agacccatgt gccgggcgga gaggagctca cccgtgattg gacgcgggtc
     2041 gccatagtca tcctggctgg gaaggcccgt cgcgacggcg tcgtacttgg acggatcctg
     2101 atcgccgtca cgcacccaca cgaggcgcat gtct
//
LOCUS       DAOSOO010000356         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_52620_length_2108_cov_5.174866, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000356 DAOSOO010000000
VERSION     DAOSOO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52620_length_2108_cov_5.174866"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLT86_14835"
     CDS             complement(<1..196)
                     /locus_tag="PLT86_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37936.1"
                     /translation="MIVALMGAMAMMAATELPMGKAPEPVAAPHFPDRLHAYVWRNWG
                     LVPMDRMAETIGAKPDDLVKM"
     gene            419..2002
                     /locus_tag="PLT86_14840"
     CDS             419..2002
                     /locus_tag="PLT86_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37937.1"
                     /translation="MREFMTKYGDKIKGVLSGFDRLVFRGTLRSLCYGAGMQAYLRHA
                     KVLLKDFGHHAEEVSARVKAAALAWASESGRPIQYLGSAKVSKEETARAIAARDGIAE
                     GPVCALTCVEPCIGFDVYKKRDEKRLEVVARPRKCLFVYHYFIHPEFGFGYVRLQTWF
                     PFRLQVGLNGRSWLARQLDAAAIGYVQAGNCFPWVGDFPRAQALLDEQLTVNWAELLD
                     EFAHQIFPLHATIFARHRVSYYWSLFQSEWATDVVFQDREVLQRLYPRWVHHGLTTFQ
                     SPDTMRFLGRTMDVCHGRVPVRFAGEVVSDVKRREEGVRLKHRMGRNSVKLYDKAYTR
                     QGSVVRVETTVNDPEDFRVFRAAEGRPEDERAWRTLRAGVADLARRAQVCQAANERYL
                     GALACVEDGPALGQLIEKVQRPVIRQGRRTRPLHPFEVPDGVLLQALSNGQFLLNGFR
                     NPDLRNLVFPTPPRDSAQARRRSAHISRALARLRVHGLVKKVPHEYRYHLTASGRRLA
                     AAVLAAKAASVSALLDKAA"
BASE COUNT          438 a          622 c          623 g          425 t
ORIGIN      
        1 ccattttcac gagatcgtcc ggtttcgcac cgatggtctc cgccatgcgg tccatgggta
       61 ccagacccca gttccgccac acatacgcgt gcagacgatc cgggaaatgc ggcgcagcca
      121 ccggttccgg cgctttcccc atcggcaact ccgtcgcggc catcattgcc atcgctccca
      181 tcaacgccac tatcatgaca ccctcccttc gcgtgagact ccgtccaaac cagaaacgaa
      241 gcatagcgcc ttgctgactg taaaaccagc cggtaacgcc cggggaaaag ccagccagtg
      301 aagacattga tccacagatt gcacagcgcg gcgtagccgc accaaaaagt gagaggcgtt
      361 tcccgaagtc gtatgctttg tttgtgaaaa gacaaatggc tcgacggagg gaaacgccat
      421 gagagagttt atgacgaagt acggggacaa gatcaagggg gtattgtcgg ggttcgaccg
      481 gctggtattt cggggtacgt tgcggtcact ttgctatggg gcggggatgc aggcatacct
      541 gagacatgcg aaagtgctct tgaaggattt cggtcatcac gcggaggaag tgtcggcgcg
      601 agtgaaagcg gcggcgcttg cctgggcgag cgagtcgggg cgtccgatcc agtacctagg
      661 gtcggccaag gtgagcaagg aggaaacggc gcgggccatc gccgcacgcg acgggatcgc
      721 ggaaggaccg gtgtgtgcgt taacgtgcgt ggaaccgtgc attgggttcg acgtgtacaa
      781 gaaacgggac gagaagcgat tggaagtggt ggcccgtcca cgcaagtgct tgtttgtcta
      841 ccactacttt atccaccccg agtttggctt cggttacgta cggttgcaga cgtggtttcc
      901 tttccgtttg caggtgggac tcaatggccg gtcgtggttg gctcgccagt tggatgcggc
      961 cgcgataggc tacgtccagg cgggtaactg ttttccatgg gttggagatt tcccgcgtgc
     1021 tcaagcgctg ctcgacgagc aactcaccgt gaactgggcg gagctgttgg atgaatttgc
     1081 ccatcagatc tttcccttgc acgcaacgat cttcgctcgg caccgggtgt cgtactattg
     1141 gagcctcttc cagagcgaat gggccaccga cgtggtgttc caggatcgag aagttctcca
     1201 acggctttac ccgcgttggg tacaccatgg cttgaccact ttccagagcc cggacaccat
     1261 gcgtttctta gggcgcacga tggatgtatg ccatggacgc gtaccggtcc gattcgccgg
     1321 cgaggtcgtc agcgatgtaa agcgacgcga ggaaggagtg cgtctcaagc accgtatggg
     1381 ccgcaacagc gtcaagctct acgacaaggc ttacacccgc caaggcagtg tggtgcgtgt
     1441 tgaaaccacc gtgaatgacc ccgaggattt ccgtgtcttt cgcgccgccg aagggcgtcc
     1501 cgaagatgag cgggcatggc gtacgctacg cgcgggtgtg gcagatctgg cgcgtcgtgc
     1561 tcaggtttgc caggcggcca acgaacgtta tctgggggct ctagcctgtg tagaagacgg
     1621 accggccttg ggccaactca tcgagaaagt ccagcgcccc gtgattcgcc agggccgccg
     1681 tacccgaccc ctacatccct tcgaagttcc cgacggtgta ctactgcaag ccctcagcaa
     1741 cggacaattc ctcctcaatg ggttccgtaa ccccgatctg cgcaacctcg tcttccccac
     1801 gccaccccgc gactccgccc aagcgcgtcg ccgttccgcc cacatctccc gagccttggc
     1861 acgcctgcgc gttcatggac tcgtcaagaa agttccgcac gaataccgct accatctcac
     1921 ggcttccgga agaaggcttg ccgccgcggt tctcgccgcc aaggccgcct ccgtaagtgc
     1981 tttgctggac aaggccgcat gacaaatcct gcgcacaaca acaagaattc aaaggttagt
     2041 agtacaatat caggcttgat acaacttgtc aagacgcttc cggtagaact cctaacacct
     2101 tgtcacac
//
LOCUS       DAOSOO010000357         2081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_53558_length_2081_cov_2.784304, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000357 DAOSOO010000000
VERSION     DAOSOO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53558_length_2081_cov_2.784304"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLT86_14845"
     CDS             complement(<1..183)
                     /locus_tag="PLT86_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37938.1"
                     /translation="MSCLLKNCTAGELHAALGPVGVSLRLARRLQAAALRQEVIPERL
                     PEVSGALLARIRGELRL"
     gene            complement(553..1281)
                     /locus_tag="PLT86_14850"
     CDS             complement(553..1281)
                     /locus_tag="PLT86_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOT37939.1"
                     /translation="MYRILKRHGGVRERRNQLRHPVYTKPELLATGPNQVWSWDITKV
                     RGPSPGLFYHLYVMLDVYSRYVVGWVLAEYESGAQAKALISLCHERQGVGPNHLTVHP
                     DRGKSMRSQEVLGLLADLQVRRSYSRPHVSNDNPYSEAQFKTLKYHPTYPQRFGSFEE
                     ARRYFGELLEWYNKQHRHSGLAFMTPEDVHLGRAEEKRRQRQQVLDRAYSEHPERFVK
                     GPPKAESVPQEVWINKPVNLSSAG"
     gene            complement(1428..>2081)
                     /locus_tag="PLT86_14855"
     CDS             complement(1428..>2081)
                     /locus_tag="PLT86_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37940.1"
                     /translation="GKKRSRAERLGIGPPASSEEATARMQTLRMYRSSSYRAYAGYEQ
                     GPEWLTVSEIRRRARGIERGEAARYRARVQDLLRAGADVSQQESFRDALGIGSAEFIA
                     RLKQIGGKGNRETERRGRLKARLSFETVVKAVAETRGEPADQWLHRHGDWGKWLTLKL
                     AREATGMTLRELGGVMGGMDYAAVCMGLRRFDERMKQKNGGETLNETYKRIRKMLYV"
BASE COUNT          354 a          749 c          578 g          400 t
ORIGIN      
        1 caggcgcagt tccccgcgga tgcgcgccaa aagggcgccg gatacttccg gaagacgttc
       61 tggaatgacc tcctgccgaa gcgccgctgc ctgcaaccgc cgcgccaacc gcagcgacac
      121 ccccaccggc cccagcgcgg catgcaattc gccggcagtg cagtttttca gcagacaaga
      181 catcgcaagt cgtgattacc ccaggcgcgg agccggatag cggttactgt ccgaactcac
      241 tacgaccccg agtatgccaa gcgattgcag aaggaaaagc ccataatgtt tcccccccgt
      301 tgcgcgccgc cccccattgg ccgcgacgct attcccggct gcaggcggaa ctcgctcgtt
      361 ctgcggcttc cggacgggct tcggatggga gcgcgctcgg gctacgcctt cgctgctcaa
      421 agggccgggc cccgcttaac agccccggga aacaacccga aaccccacaa aacagcctga
      481 aaaccaagtt ttccatggtg tggacaaatt cttcccattg tcgcttgttg tcaataccaa
      541 tgagacactt ttctacccgg cagacgacag gtttaccggc ttgttgatcc atacttcctg
      601 cggcacggac tcggccttgg gcggtccctt cacgaaccgc tcgggatgtt cggagtaggc
      661 ccggtccagg acctgctgcc gttgccgccg cttctcctcg gcccggccca gatggacgtc
      721 ttccggcgtc atgaaggcca gtcccgagtg ccgatgctgc ttgttgtacc actccagcag
      781 ctcgccgaag tatcggcggg cttcctcgaa cgatccgaac cgctggggat aggtcggatg
      841 gtacttcagc gtcttgaact gggcttcgga gtacgggttg tcgttggata cgtgcgggcg
      901 cgagtagctg cggcggacct gcaggtccgc cagcagcccg agcacctcct gcgagcgcat
      961 gcttttgccc cggtccgggt gcaccgtcag atggttcggc ccgactcctt ggcgttcgtg
     1021 gcacagcgag atcaacgcct tggcctgcgc tccgctttcg tattccgcca gcacccagcc
     1081 cacgacgtag cggctgtaca cgtcgagcat cacgtagagg tggtagaaga ggccaggcga
     1141 cggcccccgc accttcgtga tgtcccacga ccagacctgg ttcggtcccg tcgccagcag
     1201 ctccggcttg gtgtagacgg gatgccggag ctggttgcgc cgctcccgca ccccgccgtg
     1261 gcgcttcagg atgcggtaca tcgtgcgcat gctgcacagg cagatgcctt cgttcagcag
     1321 agccggcgcc ggccatcgtc ggcatcgacg agatcgccat ccgccggggc ttcacctttc
     1381 gcctggtggc caccgatctc atccgccgcc ggggtgaagg gtcacgtcta aacatataac
     1441 attttacgaa tgcgtttata ggtttcgttc agcgtttcgc caccgttctt ctgcttcata
     1501 cgttcgtcaa agcggcgcag tcccatgcag accgcggcat aatccattcc gcccattacc
     1561 ccgcccagct cccgcaaagt catgcccgtg gcttcccggg ccagtttcag ggtcagccat
     1621 ttaccccaat ccccgtgccg atgcaaccac tggtccgccg gctccccgcg cgtctccgcc
     1681 accgccttca ccaccgtctc gaagctcagc cgggccttca gccgcccgcg tctctccgtt
     1741 tcacggtttc ccttgcctcc tatctgcttg agccgggcga taaactctgc gctaccaatc
     1801 cccagcgcgt cccggaacga ctcctgttgc gaaacatccg caccggctcg cagcaaatcc
     1861 tgaacacgcg cccggtaacg cgccgcctcc ccgcgctcaa tgccccgcgc acgccgtcgg
     1921 atctccgaca ccgtcagcca ctctggcccc tgctcgtatc ccgcataggc tcgataggaa
     1981 ctcgaccggt acatccgaag cgtctgcata cgggcggtcg cctcctccga actcgccggc
     2041 ggcccaatgc ccaggcgctc cgcccggctg cgctttttcc c
//
LOCUS       DAOSOO010000358         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_54054_length_2067_cov_5.013917, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000358 DAOSOO010000000
VERSION     DAOSOO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54054_length_2067_cov_5.013917"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLT86_14860"
     CDS             complement(<1..469)
                     /locus_tag="PLT86_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37941.1"
                     /translation="MGAPYPDPSDITAYPPSDQVARHNEEARSVWAACRSDQPTRVPC
                     SGGIGLRQHQSWFGYSLKELFFDPEAAWKRQLDVQKFNRCIATGDAEIGLPEKWAPVV
                     VHWMNIAEPEWLGCPVHFEEHAAPWADFGPLRQKKELLYDMEIPGVFSTPLGRR"
     gene            complement(478..1200)
                     /locus_tag="PLT86_14865"
     CDS             complement(478..1200)
                     /locus_tag="PLT86_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOT37942.1"
                     /translation="MRYILFDIDGTLTADGDGGGAGSAALNHACRDLFGKENGFDSIQ
                     KAGKTDLIILKEGFALHGVPYTPENAQAFRDRYLYYLARFVNEDGKRAHSLPGAAELL
                     DALRERADDVAFGLLTGNWSDGARIKLGSVGLAHYFFSNGTRPLGAFGEDAPTRPDLV
                     PIAWKRFRERYGRDAEPGDTAIVGDTPRDIEAAKANGAAAIGVTTGPYSRAQLEAAGA
                     DVVLDGIADTQAVLRVLLGGGK"
     gene            1406..>2067
                     /locus_tag="PLT86_14870"
     CDS             1406..>2067
                     /locus_tag="PLT86_14870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT37943.1"
                     /translation="MFVRQRDMRSEPVRRVVALGESTTWGYSVSAKEKCWVNQVVRML
                     EEFQGAPIELVNQGIGSNVLTQECPAYPYSRGRAAVERVDEDVIALQPDMVFLSYGLN
                     DSRGGTPTEVFRRAYQQLIDRIRAGIDPTIVMLNTYYMHEVLYTECPHWEESSYNVTE
                     VYNVVIRQIAEANDCILADIYAAEVGVDWIIDNDHCHPNDLGHRIIANRVFEAIVRNC
                     SFV"
BASE COUNT          384 a          621 c          659 g          403 t
ORIGIN      
        1 tgcgcctgcc gaggggcgtg ctgaaaaccc ccgggatttc catgtcgtaa agcagttctt
       61 tcttctgccg gagagggccg aaatctgccc aaggagcggc atgttcttcg aaatgcaccg
      121 ggcacccgag ccattccggc tccgcgatgt tcatccagtg cacgacaaca ggcgcccatt
      181 tctccggcag gccgatttcg gcatcccccg tggcgatgca ccggttgaat ttctggacgt
      241 ccagttgccg tttccacgca gcttccgggt cgaaaaacag ctctttcagc gaatacccga
      301 accacgactg gtgctgacgc aacccgatgc cgccgctgca gggaacacgg gtgggctgat
      361 cgctccggca ggccgcccag accgaccggg cttcttcgtt gtgccgtgcc acctgatcgg
      421 aaggcggata cgcggtaata tccgatggat cgggataggg agcgcccatg cgggccttca
      481 ctttccgccg ccaaggagca ccctgagcac tgcctgtgta tccgcgatgc cgtccagcac
      541 cacgtctgcg ccggcggctt caagttgcgc gcgggaatag ggaccggtcg tgacgccgat
      601 agctgccgcg ccgtttgctt tcgccgcttc aatatcccgt ggcgtgtctc ccacaatggc
      661 ggtatccccg ggctctgcat cgcggccgta acgttcgcgg aaccgcttcc acgcgatggg
      721 aacgaggtcg gggcgggtgg gcgcgtcttc tccgaatgcc cccagcggcc gggtgccgtt
      781 cgagaagaag tagtgcgcca gcccgacact tccgagctta atccgcgcgc cgtcggacca
      841 gttaccggtc agtagcccaa atgcaacgtc gtcggcgcgc tcgcgcagcg cgtcgagaag
      901 ctccgccgcg ccgggcaggc tatgggcacg ttttccgtcc tcgttgacga accgcgcgag
      961 gtagtacagg taccggtcgc ggaatgcctg cgcgttttcc ggtgtgtacg gcacaccgtg
     1021 cagcgcgaag ccttctttca ggatgatgag atccgttttg ccggctttct gaatactgtc
     1081 gaacccgttc tctttgccga acaggtcacg gcaggcatgg ttgagtgccg ctgatcccgc
     1141 accgccgccg tcgccgtccg cggtaagggt gccgtcaatg tcgaaaagga tgtaccgcat
     1201 atgccttctt tgatgtcggg gggtgtgcga acgcggttca ggcctcgcga gatcttgcgg
     1261 gaacgcgcgg ccacaaaccg aaagatacgg gccaccgtgg cgggggggaa ggcgaatgca
     1321 gggagccgcc ttggactccc tgtcttcctc ttcgatcttc gtagggtaat gggcaaaccg
     1381 gggcactcat tcgaaggggt atgcggtgtt cgtcagacaa cgcgacatgc gttcggaacc
     1441 ggtgcgccgc gtggtggcgc tgggcgagag cactacgtgg ggctattccg tttccgcgaa
     1501 agaaaagtgc tgggtgaacc aggtagtgcg aatgttggag gagtttcagg gcgcgccgat
     1561 cgaactggtg aatcagggca tcggaagcaa cgtgcttact caagagtgcc cggcgtaccc
     1621 ctattcacgg ggccgtgcgg ctgttgaacg tgtggacgag gacgtgattg cgcttcaacc
     1681 cgacatggtg ttcctctcct acggattgaa cgattcacgc ggcgggacgc ccacggaagt
     1741 gttccgccgg gcgtaccagc agctcattga ccgcatccgc gcgggaatcg accccaccat
     1801 cgtcatgttg aacacgtact acatgcacga ggtgctctac acggaatgtc cgcactggga
     1861 ggaaagcagc tacaacgtga cagaggtcta caacgtggtg atccgccaga tcgcggaggc
     1921 gaacgattgc attctggccg atatctacgc ggcggaggtg ggggtagact ggatcatcga
     1981 caacgaccac tgccatccca acgacctcgg ccatcggatc atcgcgaatc gtgtgttcga
     2041 ggcgattgtg cggaactgca gcttcgt
//
LOCUS       DAOSOO010000359         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_54153_length_2064_cov_3.512693, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000359 DAOSOO010000000
VERSION     DAOSOO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54153_length_2064_cov_3.512693"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLT86_14875"
     CDS             <1..677
                     /locus_tag="PLT86_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT37944.1"
                     /translation="VTSDVGDERHYLIPYGKYILVREGDHIHAGERLSEGSANPHDIL
                     RILGPFRVQEYLLNEIQEVYRLAGVDINDKHIEIIVRQMLQKVRVVEPGDSNFLEGDL
                     VDKFRFMDENERIEEAGGQPATFEPVLLGITRASLMTESFLSAASFQETTKVLTKAAV
                     EGKTDYLRGLKENIVIGHVIPAGTGVRLHRNSTTEGGELEPLPERALDDEEEEAMLGT
                     KVLDVG"
     gene            728..1099
                     /gene="rpsL"
                     /locus_tag="PLT86_14880"
     CDS             728..1099
                     /gene="rpsL"
                     /locus_tag="PLT86_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT37945.1"
                     /translation="MPTINQLIRHGRSQVTTSTKSPDLRRCPQRQGVCTQVKTTTPKK
                     PNSALRKIARVRLTTGREVTAYIPGEGHNLVEHSLVLIRGGRVTDLPGVRYHIVRGVQ
                     DAAGVANRHKSRSKYGTKRPK"
     gene            1115..1585
                     /gene="rpsG"
                     /locus_tag="PLT86_14885"
     CDS             1115..1585
                     /gene="rpsG"
                     /locus_tag="PLT86_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT37946.1"
                     /translation="MARRRRVSKRELLPDPKYGSVVLAKFNNNMMVSGKKSTSEQILY
                     TALQMIGERTGQDEMSVFQKALDNIKPVIEVRPRRVGGSTYQIPVEVPSNRRQALAMR
                     WLIQYSRERSERTMAERLAAEIIAASNGEGNSVRKREDTHRMAEANKAFSHYHW"
     gene            1684..>2064
                     /locus_tag="PLT86_14890"
     CDS             1684..>2064
                     /locus_tag="PLT86_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT37947.1"
                     /translation="MAREYPLERTRNIGIMAHIDAGKTTTTERILLYTGRIHRAGEVH
                     DGAATMDWMEQEKERGITITSAATTCLWNEHRVNIIDTPGHVDFTAEVERSLRVLDGA
                     IGLFCAVGGVEPQSETVWRQANRYD"
BASE COUNT          487 a          558 c          647 g          372 t
ORIGIN      
        1 tcgtgacgag cgatgtcggg gacgagcgcc attacctgat tccgtacggc aagtatatcc
       61 tggtgcgcga gggagaccat attcacgcag gggagcggct ttccgaggga tcagcgaacc
      121 cgcacgacat cctgcgcatt ctggggccgt tccgcgtgca ggaatacctg ctgaacgaaa
      181 tccaggaggt gtatcgtctg gccggcgtgg acattaatga caaacacatt gagatcatcg
      241 tgcgtcagat gttgcagaag gtcagggtgg ttgagccggg cgactcgaac ttcctcgagg
      301 gcgaccttgt ggacaagttc cgcttcatgg atgagaacga acggatcgag gaagcgggcg
      361 gacagcctgc cacgttcgag ccggttcttc tcgggattac ccgggcatcg ctcatgacgg
      421 agagcttcct gagcgctgca agcttccagg agacgacgaa ggtgctcacc aaggcggccg
      481 tcgaaggcaa gacggactac ctgcgtgggc tgaaggagaa catcgtcatc ggtcacgtga
      541 ttcctgccgg cacgggtgtc cggctccatc ggaactccac gacggagggc ggcgaactcg
      601 agccgctacc ggaacgcgcg ttggacgatg aggaagaaga agcgatgttg gggacaaaag
      661 tccttgacgt cggttagttc aatcgataca tttaattgct ctgtagcttc acaaaaggga
      721 acggttcatg ccgacgatca accagctcat ccgacacgga cgctcgcagg tcaccacgtc
      781 gaccaagtcg ccagatctgc gtcgttgccc gcaacgtcaa ggggtttgca ctcaggtgaa
      841 aactaccacg ccgaagaagc cgaactccgc cctgcggaag atcgcccgtg tgcgattgac
      901 gacaggacgc gaggtgacgg cctatatacc cggcgagggg cacaacctgg tagagcattc
      961 cctcgtcctg atcagaggcg ggcgtgtgac ggatcttcct ggggttcgct accacatcgt
     1021 gcgcggcgta caggatgccg ccggcgtcgc caacagacac aagagccggt ccaagtacgg
     1081 cacgaaacgt cccaagtagg ggtgaggtag aagaatggcg cgaagacgca gggtatcaaa
     1141 aagagagctt ctgccggacc ccaagtacgg cagcgttgtc cttgccaagt tcaataacaa
     1201 catgatggtg agcggcaaaa agagcacgtc ggagcagatc ctgtacaccg ccctccagat
     1261 gatcggggag cggacggggc aggacgagat gtcggtgttt cagaaagcac tcgataatat
     1321 caaaccggtt atagaagttc gcccgcggcg tgttggcggg tcgacctacc agattcccgt
     1381 tgaagttcct tccaatcggc gtcaagcgct tgcgatgcga tggctgattc agtactcgcg
     1441 tgaacgcagc gaacggacaa tggcagaaag gctggcggca gagattatcg ctgcgtccaa
     1501 cggggaaggc aattcggttc gcaagcgtga ggacacgcac cgcatggcgg aagccaacaa
     1561 ggcgttttct cactatcact ggtaaaccca agcaccatac catataaccg cctcttgctg
     1621 tggggaggat agcatcccac gcagaggcgg gtctgtcttg gtgagaaacg acaagatctg
     1681 gacatggcac gcgaatatcc attggaacgc acgcgcaata tcggcatcat ggcccacatt
     1741 gatgcgggca agacgactac cacggaacgg attcttctct atacgggacg catccaccgg
     1801 gccggcgaag ttcacgacgg cgcggcgacg atggactgga tggagcagga gaaggaacgc
     1861 ggcatcacta ttacgtccgc cgcaacgacg tgcctctgga acgaacatcg cgtcaacatt
     1921 atcgatactc ccggccacgt ggatttcacg gccgaggttg agcggagcct tcgtgtcttg
     1981 gatggcgcga tcgggctctt ctgtgccgtc ggtggtgtgg aaccgcagtc ggagaccgtg
     2041 tggcggcagg caaacagata tgac
//
LOCUS       DAOSOO010000360         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_54181_length_2063_cov_4.258466, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000360 DAOSOO010000000
VERSION     DAOSOO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54181_length_2063_cov_4.258466"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..738)
                     /locus_tag="PLT86_14895"
     CDS             complement(160..738)
                     /locus_tag="PLT86_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37948.1"
                     /translation="MPVPVPLGASKLVAYNPERHHRRSVRLPTYDYSQPGAYFVTIVA
                     QGRECLFGQVANGSVLLSDVGKIVAEELAAAAQTYAGVACDSCVIMPNHLHVVFTITG
                     TVGAIHELPLPSKRVDRRKMLLEKVVGRFKMRSAKRINILRNASGNIVWQRNYYEHIV
                     RDENELQRNREYIRINPALWAGDRENPDNHPS"
     gene            complement(829..1947)
                     /gene="recA"
                     /locus_tag="PLT86_14900"
     CDS             complement(829..1947)
                     /gene="recA"
                     /locus_tag="PLT86_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT37949.1"
                     /translation="MAATPPSTRDSKDKAVELALGQIKKQFGDGSIMKLGDQAVATVG
                     VIPTGSIALDLALGVGGVPRGRVVELYGPESSGKTTLAMQIVAEAQKLGGVCAFIDAE
                     HAFDPTYAKLLGVDIDNLLVSQPDCGEDALGITDTLVRSGAIDTIVVDSVAALVPRAE
                     LDGEMGDQHVGLQARLMSQALRKLTGTISKSHTCVIFINQIRMKIGVMFGNPETTTGG
                     NALKFYSSVRMDIRRISTIKDGENVVGSRTRVRVVKNKVAPPFKSAEFDLMHGEGISR
                     AGELIDLGTELGIIKKSGTWFSLNDERIGQGRENAKAYLREHPELMATLTNQVREALG
                     LPTINPDGTMSPPKPVVAAEAAQVKSKIIKGGAPEAGE"
BASE COUNT          378 a          641 c          612 g          432 t
ORIGIN      
        1 tgttgcctga tcaagatacg gggcgatgcc tcatggggcg aaatggtggg gggggggagg
       61 gtgaggaggc ggcgttattg gctggcctcc tcgtagacgc ctcgcccgga aaaggtcagt
      121 gacccagcgt tgcggaatac tggcaactta aaactcgatt caggacggat ggttgtccgg
      181 gttttcccga tctcctgccc acagggcggg gttgatccgt atgtattcac ggtttctttg
      241 cagttcgttc tcgtcacgga caatgtgttc gtaataattc ctctgccaga ctatgtttcc
      301 ggacgcgttg cgcagtatgt tgatgcgttt ggcggaccgc atcttgaacc ggccgaccac
      361 tttttcgagc agcatctttc ggcgatccac acgctttgag ggtaggggca attcatgaat
      421 tgcccctacg gtgccggtga tcgtgaacac cacgtggaga tgattcggca tgatcacaca
      481 cgaatcgcac gcaacccccg catacgtctg cgcggctgca gccaattcct cggcgacgat
      541 tttccccaca tccgacagca aaacggaacc gttcgccacc tggccgaaca ggcattcacg
      601 gccctgtgcc acaatggtga cgaaatacgc ccccggctgg gaatagtcgt acgttggcag
      661 tcgaacggaa cggcgatgat gtctctcggg attgtacgcc acgagtttgc tggctcccag
      721 cgggactggt acgggcaatt tgtgaaatgt ccccaccgct tacaccatcg tcgccgactg
      781 aagtgatgag actccgttcg accgttcgat tgcgtgtgga gctgccgctc actcccccgc
      841 ttcgggcgcg ccgcccttga tgatcttgct cttcacctgc gccgcttccg ccgcaaccac
      901 cggtttcggg ggcgacatgg tgccgtcggg gttgatggtc ggcaggccaa gcgcctcccg
      961 cacctgattc gtcagcgtcg ccatgagttc cgggtgctcg cgcaggtaag ccttcgcgtt
     1021 ctcgcgtccc tgcccgatgc gctcgtcgtt cagcgaaaac cacgtgcccg atttcttgat
     1081 gataccgagc tcggtgccga ggtcaataag ctcgccggcg cggcttattc cctcgccgtg
     1141 catgaggtcg aattccgcgc ttttgaacgg cggcgcgacc ttgttcttca ccacgcgcac
     1201 cctggtccgg ctgccgacca cgttctcgcc gtccttaatc gtggagatac gccggatatc
     1261 catgcgcacc gacgagtaga acttcagcgc attcccgccc gtggtggttt ccggattgcc
     1321 gaacatgacg ccgatcttca tgcggatctg gttgatgaaa atgacgcacg tgtggctctt
     1381 ggagatggtg ccggtaagct tgcgcagcgc ctgggacatg agccgcgcct gcaaacccac
     1441 gtgctggtcg cccatttcgc cgtcgagctc cgcgcgcggc accagcgcgg cgacggaatc
     1501 caccacgatg gtgtcaatag cgccgctgcg gacgagggta tcggtgattc ccagcgcgtc
     1561 ctcaccgcaa tcgggctggc tgacgagcag gttatcaata tccacgccga ggagtttggc
     1621 gtaggtgggg tcgaaggcgt gctcggcgtc aatgaacgcg cacacgccgc ccagtttctg
     1681 cgcctctgca acgatctgca tcgccagcgt ggttttgccg gacgactccg ggccgtacag
     1741 ctccacaaca cgtccccgcg gcacaccgcc aacgcccagc gccagatcga gcgcgattga
     1801 cccggtcgga atcacgccca ccgtggcaac cgcctggtcg ccaagcttca tgatcgagcc
     1861 gtcgccaaac tgcttcttga tctggcccag cgccagttca actgccttgt ccttcgaatc
     1921 gcgtgtcgac ggaggtgtgg ctgccatggg tggctccttg tgtcaggact cctgaaaagg
     1981 gacaaacagc taaaggatga taccgcgccc cgccgcggtc aaggacgctc tggaacacga
     2041 cgatacgatt cacgcgaaac gca
//
LOCUS       DAOSOO010000361         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_55026_length_2040_cov_4.131486, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000361 DAOSOO010000000
VERSION     DAOSOO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55026_length_2040_cov_4.131486"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLT86_14905"
     CDS             <1..839
                     /locus_tag="PLT86_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT37950.1"
                     /translation="VRFDDVGGQDKAVSDIRDWVELPLRHPELFQHVGVPTQVGVILF
                     GPPGTGKTLLAQAVAGECEAHLEMVSGPEILSRWVGQSEEAIRIIFERARSMAPSVVL
                     LDEIDAIAPARDKTYHGHEITLVSQLLAVMDGLYERGQVVVIATTNRLEAVDPALRRP
                     GRFDYAVEMGLPDLQGREAIFQRHIRRMAVGEVNIRELAERTPGMSGADIAKICRQAG
                     LLCAKEAIAKGYDEVADVIVEARHFMAALGETGTGAPVPDAGSTTRSGRSKRRTRYHG
                     EA"
     gene            839..1450
                     /locus_tag="PLT86_14910"
     CDS             839..1450
                     /locus_tag="PLT86_14910"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOT37951.1"
                     /translation="MTQPAVLDAAAALSSFMDLKKGRLSACTAQHRGRLSDLCGVFSD
                     EEARARKAMEDDETVYESFLTEPADQPRARALCYSTTVVYPGRIGDEFYMTRGHRHAR
                     QDSPEVCLTARGRGMVLLQTDDFRIQALALEPGTVVYVPGVTSHRIVNTGDELLAVFS
                     VFPLMAGRNYRAVESHGFRRIVVATPEGPDLVPNPQFRPGKGE"
BASE COUNT          414 a          587 c          651 g          388 t
ORIGIN      
        1 cggtgcgctt tgacgacgtc ggcggacagg acaaagccgt ttccgacatc cgggactggg
       61 tagaactgcc cctccgtcac cccgagcttt tccagcacgt gggcgtgcca acccaggtgg
      121 gagtgattct cttcgggcct cccggtaccg gaaagacgtt gctggcccag gctgtcgccg
      181 gagaatgcga ggctcacctc gaaatggtga gcgggcctga aatcctttcc cgatgggtag
      241 ggcagtctga agaagcgatt cggattattt tcgagcgcgc gaggagcatg gcgccgtcag
      301 tagtgttgtt ggacgagatt gacgccatcg cgccggcacg ggacaagacc tatcacgggc
      361 acgagataac cctcgtatcc cagttgttgg ccgtgatgga cgggctgtac gagagaggac
      421 aggtcgtggt gattgccacg acgaaccgtc tggaagcagt ggatcccgca cttcgccggc
      481 cggggcggtt tgattacgct gttgaaatgg gccttcccga ccttcagggc cgcgaggcga
      541 tcttccagcg acacatccgg aggatggcgg tcggggaggt gaatatccgc gaactggcag
      601 aacgtacgcc ggggatgtcc ggcgcggata tcgcgaaaat atgtcgtcag gcaggacttc
      661 tgtgcgcgaa ggaagcgatt gcgaaagggt atgatgaggt tgcggacgtg attgttgaag
      721 cgaggcattt catggcggca ttgggggaaa cgggaacagg agcccccgtt ccggatgctg
      781 ggtccacaac tcggtccggg cgctcaaagc gtagaacgcg ttaccacgga gaggcttgat
      841 gacgcaacct gctgttctgg acgccgctgc cgccctttcg tcattcatgg acctgaaaaa
      901 aggccgtctg agcgcatgta ccgcgcaaca ccgagggaga ttgtcggatc tgtgcggggt
      961 gttctcggat gaagaagcgc gcgcccgcaa agcaatggaa gacgacgaaa cggtgtacga
     1021 gtcgtttctg accgagccgg ctgatcaacc ccgcgcgagg gcactttgtt actccaccac
     1081 agtcgtgtac ccgggccgga tcggcgacga gttctatatg acccgcggcc acagacacgc
     1141 gcggcaagac tcacccgagg tgtgcctgac ggcaagaggg cggggtatgg tcctcctcca
     1201 gactgacgat ttccggattc aggcccttgc gctcgaaccg ggtactgtgg tgtatgttcc
     1261 aggagtaacc tcgcaccgga tcgtgaacac gggcgatgaa ctcctggcag ttttctccgt
     1321 gtttcccctc atggccgggc gaaattacag ggcggtggag tcgcacgggt tcaggcggat
     1381 cgttgtggca accccggagg gtcctgatct tgtgccgaat ccgcagttcc gcccggggaa
     1441 aggcgaatag aacgcgcgac agcagcgtgt tcctgaggct gcggggaccc tctcaggcgt
     1501 tatccgccac gcttcgacgc tgctgacaac accgcgatgc tcgagaagtt cccaccagaa
     1561 agaaagagaa ccggaccgga actcccgatc acggcctctc ccggctgcgc agttcgccgg
     1621 tatgactgca cagccctttt tcgaagagaa catcaaccgg cgtatttgca cccttttacc
     1681 ccctttccac tcacgttagt tccacccggc accatgcgcg tcttgagacc ggtgccgggc
     1741 ggagccggaa ccccgtgcaa tgcactactc ccttgacccg cttcacgcgg caacggttac
     1801 ttaactggcg tgatcccggt cgaagatccc gatcgcctcg ggagatttgg cgacggcagg
     1861 ggcgtgaatt caatcggcga agacgaaacc gcagattgcc cgcagccggg ttacatgagg
     1921 cccgcgaatc atcactacct tccgatccgc cagcatctgg agcgcacgtg ggatgcgatg
     1981 gcacgtcagc gttgacggac tctcgctttt gtagggcagc agcaacagga gacttcggat
//
LOCUS       DAOSOO010000362         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_55296_length_2033_cov_5.602629, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000362 DAOSOO010000000
VERSION     DAOSOO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55296_length_2033_cov_5.602629"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLT86_14915"
     CDS             <1..237
                     /locus_tag="PLT86_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37952.1"
                     /translation="SNIGDLAGADYVIVGRVAQIEDMYSVTLRIVETATSETVASAAI
                     ECSPCDFSAVMRESMRAAAVALRDDFQRRLRSRQ"
     gene            complement(440..>2033)
                     /locus_tag="PLT86_14920"
     CDS             complement(440..>2033)
                     /locus_tag="PLT86_14920"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT37953.1"
                     /translation="ELLRIAEICRRVPAWPARTFHEAAQTLWFIHLWIAQELGGGHGC
                     FCPGRVDQYLYPYYEADVRAGRLSREDAYEILTCLLLKFNEFDPYSTPQTLFVGGQHA
                     DGSDASNELSLLCLEVSRDLQMLHPALCVSWHRNLNRDVMRRAVELMGTGIGFPAVFN
                     DEVIVPGLMRDGASREEAVNYMAGSCVEISAIGCSNPWVASGYVNAGKAFERAFQRVA
                     RNKNAGWDDLVREFKADLAEAMRFNYEITAAHDHAWQECIRYPFLSCLVNDCIERATD
                     ITCGGARYNTTMPELVGLANVVDSLMAVKWACFEEKAMTLPQLAEIVRKNWEGEEALR
                     RVLGSKPPRFGNDMPEPDALWVALSEFWFDEVRKYTNPRGGPYQPGFLCWVMHSVLGD
                     KTGATPDGRKAREALADAVGPVQGHDTSGVTAMLRSAAKMDHSKFIGGIVTNVKFTPA
                     MFETAESREKIIDLLESYLHLGGFELQINVVSADTLRAAQEHPEKYSDLIVRVGGYSD
                     YYTRLSRSLQDEIIKRTEHVAG"
BASE COUNT          364 a          649 c          634 g          386 t
ORIGIN      
        1 tcgaacatcg gcgacctggc aggtgcagat tacgttatcg tcgggcgtgt ggctcagatc
       61 gaggacatgt attcggtcac gctgcgtatt gtggaaacgg cgacgagcga aaccgtagcc
      121 tcggcggcga tcgagtgctc gccgtgtgat ttcagcgcag tgatgagaga atctatgcgt
      181 gcggctgcgg tcgcgttgcg ggacgatttc cagcgtagac tgaggagccg gcagtagagc
      241 gcgccccaaa agcggggtgc atttttaccg cctcaccgcg gaagagggca cggtgacgcg
      301 gcgaatgacc ttggttaggt agaaacgcgc ttggtggctg agcccgtcga agccccgcgc
      361 gccctggacg cctgtcgcca aaaaatgtag gggcgatgca tgcgtcgccc ctaccgtcaa
      421 acatgccgtg cggtgcgcgc taccccgcca catgctccgt gcgcttgatg atttcgtcct
      481 gcaacgaccg tgacagacgg gtgtagtagt ccgagtaccc cccaacccgc acgatgaggt
      541 cgctgtactt ctccgggtgc tcctgcgccg cgcgcagggt gtcggcggac accacgttga
      601 tctgcagttc gaacccgcca aggtgcaggt agctctccag caggtcgatg atcttctccc
      661 tgctttccgc cgtttcgaac atcgccgggg tgaacttcac gttggtaacg atgcccccga
      721 tgaacttcga gtgatccatc ttcgcggcgg agcgcagcat cgccgtcaca cccgaggtat
      781 cgtgcccctg caccgggccg accgcgtccg caagcgcctc gcgcgcttta cggccgtccg
      841 gcgtcgcgcc ggttttgtcg cccagcacgg aatgcatcac ccagcagagg aagccgggct
      901 gataggggcc cccgcgtggg ttcgtgtact tccgcacttc atcgaaccag aactccgaaa
      961 gcgccaccca gagcgcgtct ggttccggca tgtcgtttcc gaaccgcggc ggcttcgaac
     1021 cgagcacgcg acgcagcgcc tcttcgcctt cccagttttt ccgcacgatt tccgccagtt
     1081 gcggcagcgt catcgccttt tcctcaaagc acgcccactt gacggccatc aggctgtcaa
     1141 cgacgttggc gaggccgacc agctcgggca tggtcgtgtt gtaccgcgcg ccgccacagg
     1201 tgatgtcggt cgcccgttcg atgcagtcgt tcacaagaca ggacaggaac gggtagcgga
     1261 tgcactcctg ccaggcgtgg tcgtgcgccg cggtgatctc gtaattgaac cgcatcgcct
     1321 cggcgagatc ggccttgaac tcgcgcacga ggtcatccca cccggcgttt ttgttgcgtg
     1381 cgactcgctg gaaggctcgt tcaaaggctt ttccggcgtt cacatacccg gacgccaccc
     1441 acggattgga gcatccgatc gccgagattt ccacgcagga accggccatg tagttgacgg
     1501 cttcttcgcg gcttgcgccg tcccgcatca gccccggcac gatcacttcg tcgttgaaca
     1561 ctgccggaaa tccgatcccc gtgcccataa gttccaccgc ccggcgcatg acatcgcggt
     1621 tcaggttcct gtgccagctt acgcagagcg caggatgaag catctggaga tcgcgcgaca
     1681 cctcgaggca cagcaggctg agttcgttgg atgcgtcgga gccatccgcg tgctgcccgc
     1741 cgacaaagag agtctgcggc gttgaatagg ggtcgaactc gttgaacttg agcagcaggc
     1801 aggtgaggat ttcgtacgcg tcctcccggg agagccgccc ggcacgcaca tccgcctcgt
     1861 aatacgggta caggtactgg tctacccgtc cggggcagaa acagccgtgc ccgccgccga
     1921 gctcttgagc gatccagagg tggatgaacc agagcgtctg cgcggcttcg tggaaggtcc
     1981 gcgccggcca ggcaggcacg cggcgacaaa tttcggcgat gcggagcagt tcc
//
LOCUS       DAOSOO010000363         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_55373_length_2031_cov_4.350202, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000363 DAOSOO010000000
VERSION     DAOSOO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55373_length_2031_cov_4.350202"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLT86_14925"
     CDS             <1..939
                     /locus_tag="PLT86_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT37954.1"
                     /translation="GWWDQTIARWHTEGLPADLTDRYDICRHFGLDIYKQDWFGITKP
                     GFPSPKSHGAGVIETEADYDRVREFLYPDNPVDPRRWEALAAEQATGEVVLWFSLNGF
                     FWFPRTLLGIQRHLLAFYDQKELMHRINTDLLEWNLKVIDQLCAVCTPDWMTFAEDMS
                     YNYGSMLSKQAFDEFMLPYYRQIVPKLKERGIITIIDSDGDITVPAHWFEEAGLEGIL
                     PLERQAGVDIAKLRREHPRMRFIGHFDKMTQDKGEDVMRAEFERLIPTAAKGGFLPSC
                     DHQTPPGVSYEDYKVYIRLFQEYGEKAGKMSRGLAG"
     gene            complement(951..1331)
                     /locus_tag="PLT86_14930"
     CDS             complement(951..1331)
                     /locus_tag="PLT86_14930"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOT37955.1"
                     /translation="MFQDSRGPIEAFDWGEFTIVGKQHRKGKGAGKDVRVVGTEVSEW
                     EERKGHLLTPDMITGVFKHGIEVLIIGNGVHGLLECPAKVREKIVAKGIPDVRVLPTP
                     DACREYNRLFHEGRKVALLAHGTC"
     gene            complement(1303..1641)
                     /locus_tag="PLT86_14935"
     CDS             complement(1303..1641)
                     /locus_tag="PLT86_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37956.1"
                     /translation="MKRCTAWLAVAGLATLMASCATQSSLPVADPPGFWRGLFDGLVI
                     FFSLIASIFTDIRIYAYPNAGGWYDFGYFLGVSAALGGSATARAGSGKKRKPRGNGGG
                     NDVSGQPGSH"
     gene            complement(1712..1978)
                     /locus_tag="PLT86_14940"
     CDS             complement(1712..1978)
                     /locus_tag="PLT86_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HOT37957.1"
                     /translation="MKLDVKALALTSGILWGLAIFILTWWIIAFDGSSESITFLGKIY
                     RGYAVTPLGSIIGLIWGLVDGLICGAIFAWVYNKMLACCEKKTT"
BASE COUNT          389 a          627 c          639 g          376 t
ORIGIN      
        1 ggatggtggg accagaccat cgcgcggtgg cataccgagg ggctgcccgc cgacctgacg
       61 gaccggtacg atatctgccg gcatttcgga ctcgatatct acaaacagga ctggttcggc
      121 atcaccaaac cggggttccc ctcgccgaaa tctcacgggg ccggggtgat cgagaccgag
      181 gccgactacg atcgcgtgcg ggagttcctg tatcccgata acccggtgga tccacggcgc
      241 tgggaagctc tggcggcgga gcaggcaacc ggggaggtgg ttctctggtt cagcctgaac
      301 ggcttcttct ggtttccgcg gacgctgctc ggcatccagc gtcacctgct ggcgttctac
      361 gaccagaagg agctgatgca ccgcatcaac accgacttgc tggaatggaa cctgaaagtg
      421 atcgaccagc tttgcgcggt ctgcacgccc gactggatga ccttcgcgga ggacatgagc
      481 tacaactacg gctccatgct ctccaagcag gcgttcgatg agttcatgct gccgtactac
      541 cggcagattg tgccgaagct gaaggaacgc gggatcatca ccatcatcga ctccgatggc
      601 gacatcacgg tgccggcgca ctggttcgag gaggcggggc tggagggcat tctgccgctg
      661 gaacggcagg ccggcgtgga tatcgcgaag ctgaggcgcg agcatccccg catgcggttc
      721 atcgggcatt tcgacaaaat gacgcaggac aagggcgaag acgtgatgcg cgcggagttc
      781 gagcggctca tccccaccgc ggcaaaggga ggcttcctcc ccagttgcga ccaccagacg
      841 ccgcccggcg tgtcatacga agactacaag gtctacatca ggttgtttca ggagtacggg
      901 gagaaggcgg ggaagatgtc gcgggggctg gcggggtagg cgagaggtta tcaacacgtt
      961 ccgtgcgcca gcagggcaac cttgcggcct tcgtggaaca ggcggttgta ctcccgacat
     1021 gcgtccgggg tcggcagcac gcgcacgtcg ggaatcccct tcgcgacgat cttctcgcgc
     1081 accttcgccg ggcactcaag caggccgtgc acgccgtttc cgatgatcag cacctcgatg
     1141 ccgtgcttga acacaccggt aatcatgtcg ggcgtaagca ggtgtccctt ccgctcctcc
     1201 cactcgctta cctcggtgcc gacgacgcgg acatccttcc ccgcgccttt tccctttcgg
     1261 tgctgtttcc ccacgatggt gaactcgccc cagtcgaatg cctcaatggg accccggctg
     1321 tcctgaaaca tcgttgcccc ctccgttccc gcgcggcttc cgtttcttcc cgcttcccgc
     1381 gcgtgcggtt gcgctgccgc ccagggccgc gcttaccccg aggaagtaac cgaagtcgta
     1441 ccatccgccg gcgttgggat aggcgtagat ccggatatcc gtgaagatgc tcgcgataag
     1501 gctgaaaaag atcacaaggc cgtcgaacaa cccgcgccag aaccccggag gatcggcgac
     1561 agggagggag gattgcgtgg cgcaggaggc catcagggtt gcgagacccg ccactgcaag
     1621 ccacgcggtg cagcgtttca ttccggcttc tctcctcccg tggaagggaa tgcgtggcgg
     1681 cccgacgggc acggagcgtg cccgaaaatg ttcaggttgt ctttttctcg cagcacgcga
     1741 gcattttgtt gtagacccac gcgaatatgg cgccgcagat gagtccgtcg acgagccccc
     1801 agatgagccc gataatgctg ccaagggggg taacggcata gccccggtag attttgccga
     1861 ggaatgtgat gctctcggat gagccgtcaa acgcgatgat ccaccaggtg aggatgaaaa
     1921 tggcaaggcc ccacaggatg ccgcttgtaa gcgcgagcgc tttgacatcg agcttcatgc
     1981 gttgctcctc ctttcacctc aactccaccg gcgggcacgc tccgtacgcg g
//
LOCUS       DAOSOO010000364         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_55374_length_2031_cov_4.141194, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000364 DAOSOO010000000
VERSION     DAOSOO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55374_length_2031_cov_4.141194"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLT86_14945"
     CDS             <1..448
                     /locus_tag="PLT86_14945"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT37958.1"
                     /translation="TMPKTILVVYHTMSGNTAAAAAAVAEGIRSVPGVEAVVKLAADA
                     DENDLLACDAVCLGTPDYHNYMAGMLKDFLDRTYYPTEGKVTGKPYGAFVTHGGDGHA
                     VESVETVCGYFKFRRVAPTASVFGRPDEAAERELRHLGETLAKAVA"
     gene            complement(1124..>2031)
                     /locus_tag="PLT86_14950"
     CDS             complement(1124..>2031)
                     /locus_tag="PLT86_14950"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOT37959.1"
                     /translation="ETLMPYAHYDKRWLRPAGTTEDGIERQRLMATAIIEKGDRITAD
                     DLAAVWRRDIRGENFGVIMEPCDKPLWEKAMEGVPGEEIGQATPYPGVNSFPRSSHCV
                     GIMNAGDPDQAARDALAIGRIYQKAEGESLQWAAGMDAGIAEALKPEATVESVLEAIR
                     SHMPPYIQKKMDFAQEMAAKYDDVFAMREEFYRVWNNTEGVYPMSMAEEVVNKGFAVF
                     RVAKGDPKACIIGGVNFGRDTDCLAAIAGGLAGALNGSASIPQEWIDTVDAATKINPY
                     TVSRRTLRETADGLYEVVMKIRAGR"
BASE COUNT          351 a          712 c          613 g          355 t
ORIGIN      
        1 caccatgcca aaaaccatcc ttgtcgtcta ccacaccatg agcggcaaca ctgcagccgc
       61 tgccgcggcc gtggcggagg gaatccgatc ggtgcccggt gtggaggcgg ttgtcaagct
      121 ggccgctgac gccgatgaga acgatcttct cgcctgcgac gcggtctgcc tcggcacgcc
      181 ggactaccac aactacatgg ccggcatgct gaaggacttc ctcgaccgga cgtactaccc
      241 gactgagggg aaggtcaccg gcaaaccgta tggcgcgttt gtcactcacg gaggggatgg
      301 tcacgcggtg gagagtgtgg agacggtctg cgggtacttc aagttccgcc gcgttgcccc
      361 gacggcgtcc gtgttcgggc ggccggacga ggctgccgag cgagaattac gtcatcttgg
      421 ggaaacgctc gccaaagcag tggcctgatc aaggtcttgg ccggacaggc aagaatgcct
      481 gtcccacctc cacagaatgg cgcctcgcgg gcagccagaa acagcacacc ggggggcagg
      541 catccctccc ctcccgtggc gggcccgcct gccgagaacc cggacacccc aaagcgccag
      601 cggcgcgcaa tagctcaacc cggttcgcag gaccgggcgt gggttgtcaa tagccctgga
      661 ttcccgcctg cgcgggaatg acgttgtgcc gcgtgggttg aggaagagag caaccagtga
      721 ctgcgggaga agggctcgtg cctgccggcg ttgaccaaag ccgtcccgct atgccgcccg
      781 tagcgttcgg aatcgtcatt cccgcgaagg cgggaatcca gaaagccgtt acgtcgccac
      841 ctgagaccaa tctaccgatc ctcgccgctg cgccaaggcg gcggacggca ggtttggcag
      901 gcacgcacgt gtcgtgcctg caccgcgtgc gctcctgccc gtctcacccg caaaaccgca
      961 tcatgacggt gggacagtcc ggtgccagaa cactatgaag cgccaacggc gcgcaatagc
     1021 tcagcccggt tcgcaggacc gggccggaac gcacggcccg tagccccctg ccgtttccat
     1081 ccgtcctgaa cgaccgatac gccccgaaaa acgggcaccc ccgtcaccgt cccgcgcgga
     1141 tcttcatcac cacttcatac agcccgtccg cggtttcgcg cagcgtccgc cggctgacgg
     1201 tgtacggatt gatcttcgtc gcggcgtcca ccgtgtcaat ccactcctgc gggatactcg
     1261 ccgacccgtt caacgcaccg gccagcccgc ctgctattgc cgcgaggcag tccgtgtccc
     1321 gcccgaagtt cactccgccg atgatgcagg ccttcgggtc gcccttcgcc acgcggaaca
     1381 cggcaaatcc cttgttcacc acctcttccg ccatggacat ggggtacacg ccctcggtgt
     1441 tgttccacac gcggtagaac tcctcgcgca tggcaaacac gtcgtcgtat ttcgctgcca
     1501 tctcctgcgc aaagtccatc ttcttctgga tgtacggcgg catgtgcgaa cggatggcct
     1561 ccagcacgct ttccactgta gcctcgggtt tcagcgcttc ggcgatgccg gcatccatcc
     1621 cggcggccca ctggagcgac tcgccctccg ccttctggta gatgcgcccg atcgcgagcg
     1681 cgtccctcgc tgcctgatcg ggatcgcccg cattcatgat gccgacgcag tgcgatgaac
     1741 gggggaacga attcaccccc ggatacggcg tcgcctgccc gatttcctca ccggggacac
     1801 cctccatcgc cttctcccac agcggcttgt cgcatggctc catgatgacg ccgaagttct
     1861 cgccccggat atcccgccgc cacaccgccg caaggtcatc cgcggtgatg cggtcgccct
     1921 tctcgatgat ggccgtcgcc atgagccgct ggcgctcgat gccgtcctcg gtggttccgg
     1981 cggggcgcag ccagcgcttg tcgtagtggg cgtacggcat gagcgtctcc a
//
LOCUS       DAOSOO010000365         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_56025_length_2014_cov_4.095457, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000365 DAOSOO010000000
VERSION     DAOSOO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56025_length_2014_cov_4.095457"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLT86_14955"
     CDS             <1..830
                     /locus_tag="PLT86_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37960.1"
                     /translation="PEYRRERIAILKEAMEIGVDGLMLDFVRQPPALRYHPALVNRYR
                     ELTGRDARFLDPSERDSVLDWCRFRAGFVTDLLRELKAALDPFRQKWGKRVPVQVRIP
                     NDGFEANMIAGFDVQTWCHEGLVDELSLSELHWLPGYTDWDDKPYIALGKQRCIPVFG
                     SSNCLPVQRGGWGGKINPFGINPLVLARRVLKSMEDGADGTCLYQSDTGAFLPGVSEA
                     IAAFGSEETLRRYVNDSAIIAANPVTEQNQEYGIDNHSDSRENLAKRIEASSDAAWL"
     gene            complement(874..>2014)
                     /locus_tag="PLT86_14960"
     CDS             complement(874..>2014)
                     /locus_tag="PLT86_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT37961.1"
                     /translation="GFQMTDWMLYTDVWANWTAGAGWIAQHFFDYWLFTGDREFLLRR
                     AIPFMKEVALFYEDFLFEGTDGRLVFSPSLSPENIPDRPGASHAQINATMDVAVAREL
                     LTNLCSACELMDVECEGVARWRKLLEKLPAYETNKDGAIREWIHPDLPDNYHHRHLSH
                     IYPLFPGLEVTRETEPELFEACRLAVEKRLVIGLSSQSGWSYAHMANIYARLGNGDRA
                     LDCLENLCRSVVGPNLFTYHNDWRQQGLTVGWFGSQPPFQIDANLGISAAVLEMLVFS
                     VPGMIKVLPALPDSWRSGSASRIACRGGGLVSIQWDLDAGVVDVHFQSKTDRPIVFQF
                     PFRVTDLEVSGASQVSESPLGAHYREVTPVAGADVRMRAVTASGS"
BASE COUNT          411 a          623 c          601 g          379 t
ORIGIN      
        1 tccccgaata ccgccgcgaa cgcattgcca ttctcaaaga ggcgatggag atcggcgtgg
       61 acggcctgat gctcgatttc gtccgccagc ctcccgcgtt gcgctaccac cctgctctgg
      121 tgaaccgtta ccgggagttg accggcaggg acgcgcgatt tctggaccct tcggaaagag
      181 acagtgtact tgactggtgc cgtttccgcg ctggtttcgt tactgatctg ttgcgcgaac
      241 tcaaagcggc gcttgatccg ttccggcaga aatggggtaa gcgcgtgcca gttcaagtgc
      301 gcatcccaaa cgacggcttc gaagcgaaca tgatcgcggg gttcgatgtc cagacatggt
      361 gccacgaagg tctggtagac gagctttccc tgagcgaatt gcactggttg cccggctaca
      421 ccgactggga cgacaagccc tacatcgctc tcggaaaaca gcgctgtatt ccggtgttcg
      481 gcagcagcaa ttgccttccc gtacaacggg gtggctgggg cggaaagatc aacccattcg
      541 gcatcaaccc gttggtgctc gcgcggcgtg ttttgaaaag catggaggat ggtgccgacg
      601 ggacgtgcct ttaccagagc gatacgggag cgttcctgcc gggggtttcc gaagcgatcg
      661 ccgcgtttgg ctctgaagaa acgctgcgcc ggtatgtgaa cgattccgcg atcatcgccg
      721 ccaaccccgt gacggaacag aaccaggaat acgggatcga caaccattcc gacagccgcg
      781 agaacctcgc aaagcgaatc gaagccagca gcgatgccgc gtggctgtga ttcgggtgct
      841 gacgggatgg gtgcgcccga atgagcgcgt ttttcagctt cccgaggccg taactgctct
      901 catgcgcacg tctgcgccag ctacgggtgt cacctcgcgg taatgcgcac cgaggggtga
      961 ttctgatacc tgcgatgccc ctgatacctc gagatccgtc acccggaacg ggaactggaa
     1021 cacgatcggg cggtcagttt tgctttggaa gtgaacgtcc accacgccgg catccagatc
     1081 ccactgtatg gaaacgagcc ccccgccgcg acacgcaatc cgggaggcgc tgccagaccg
     1141 ccatgagtcc ggcaacgccg gcaggacctt gatcatcccg ggtaccgaaa acaccagcat
     1201 ctcgagaacc gcagccgaga tgcccagatt ggcgtctatc tggaacggcg gttgcgaccc
     1261 gaaccacccc accgtgagcc cctgctgccg ccagtcgttg tgatacgtga aaaggttggg
     1321 ccccaccaca gatcggcaca ggttttcaag gcaatcaagc gccctatcgc cattgcccag
     1381 gcgggcatat atgttcgcca tgtgggcata cgaccacccg ctctgcgatg acaagccgat
     1441 caccagccgc ttttccacgg ccaggcggca cgcctcgaaa agctccggct cggtctccct
     1501 cgtcacctca agcccgggga acagcgggta gatgtgggac aagtggcggt ggtggtagtt
     1561 atcgggcaga tcggggtgga tccactcacg aatcgcgcca tccttgttcg tctcatacgc
     1621 agggagcttt tccagcaact tccgccaacg cgcgacgcct tcgcattcca cgtccatcag
     1681 ttcgcacgcg gaacacaggt tcgtcaacag ctccctcgcg accgccacgt ccatcgtcgc
     1741 attgatctgc gcgtgcgaag caccgggtct gtcggggatg ttttccggcg agagtgaggg
     1801 cgagaacacg agcctgccgt ctgtgccctc gaaaaggaag tcctcgtaga acagcgccac
     1861 ttccttcatg aacggaatgg cgcgccgcag gaggaactct ctgtccccgg tgaacagcca
     1921 gtagtcgaag aagtgctgcg ctatccagcc tgcgccggca gtccagttgg cccacacgtc
     1981 agtgtagagc atccagtcgg tcatctggaa gccg
//
LOCUS       DAOSOO010000366         2000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_56522_length_2000_cov_5.617995, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000366 DAOSOO010000000
VERSION     DAOSOO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56522_length_2000_cov_5.617995"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLT86_14965"
     CDS             <1..182
                     /locus_tag="PLT86_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37962.1"
                     /translation="VRFLPRNTSTGNTAGTGSPRARKLAAIRRREVVDPNTEIVQVLD
                     EPFRFLPLFPLTMTL"
     gene            187..1485
                     /locus_tag="PLT86_14970"
     CDS             187..1485
                     /locus_tag="PLT86_14970"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT37963.1"
                     /translation="MTFSEATRYLNALIDYEKSPDFSYATAFKLDRVQSLMATMREPF
                     RSFDSVHIAGTKGKGSVAIYCEALLRASGIRTGLFTSPHLVDFRERIRVNGARISESL
                     FADITTEIQPHVARWQADHPDDRLSFFDLLTAIGFEAFRRSEVEIAVIEVGMGGRLDA
                     TNVLLPKVSVITPIALEHTRFLGPTVEAIAAEKAGIIKEQVPVVVAEQKPEAAAVIAR
                     VAAGKHAPSTWVGRDITFDETGEPWAVRLKARSVDGVALKMQGVHQRHNFATALAALD
                     EIGCVPGEAVVRKVASETILPGRMQVLPTRPPCMLDVAHTPESAACLAEGIRQTFAGK
                     RVTLVFSCASDKQVEGIAQVLAPVAGNVVIVPMSGPRAMSMERMYDAWSRAHTRVERA
                     ETISAAVDRLKDAGSDSAWVVCGSFVLVGAAMQLLGYSPP"
     gene            1557..>2000
                     /locus_tag="PLT86_14975"
     CDS             1557..>2000
                     /locus_tag="PLT86_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT37964.1"
                     /translation="MSEKLAIHGGTPVRTKPMPPANPGECIIGEEEKEQVLEVLDAHS
                     PFRFYGPDLRKKTEQFEAEFEEFMGVKHALGVTSGTAALKVALAAAGVGPGDEVIVPA
                     VTFIACAGAVVMSRARPVFANVGTDIGLDPADVERKITKRTAAIMP"
BASE COUNT          417 a          556 c          611 g          416 t
ORIGIN      
        1 tggtccgttt ccttccgcgg aacacgtcca cggggaatac ggcaggcacc ggcagccctc
       61 gtgcgagaaa actcgccgcg attcggcgtc gggaggttgt tgacccaaac accgagatcg
      121 tgcaggtttt ggatgagccc tttcggtttt tgccgttgtt cccactcacc atgacactct
      181 gatccgatga cgttttctga agcaacgcgt tacctcaatg cactgattga ctacgagaag
      241 tcccccgatt tttcgtacgc aaccgccttc aagctcgacc gcgttcaatc gctcatggcg
      301 accatgcggg aaccgttccg gtcgttcgac tccgtgcaca tcgcgggcac caaaggcaaa
      361 ggctccgtcg cgatctattg tgaggccctt ctccgtgctt ctggaatcag aaccgggctg
      421 ttcacctcgc cccacctggt ggattttcgt gagcggattc gagtcaacgg cgccagaatc
      481 agcgagtcat tgtttgcgga catcaccacg gaaatccagc cgcacgttgc cagatggcag
      541 gcagatcacc cggacgaccg tctctcgttc ttcgatctcc tcactgccat cggttttgaa
      601 gcattccgtc ggagcgaagt cgaaattgcc gtcatcgagg tgggtatggg cggccgcctc
      661 gacgcgacga acgtactcct tccgaaagtt tccgtgataa caccgattgc gctggagcat
      721 acgcgctttc tgggcccgac agtagaagcg attgccgcgg aaaaagccgg gatcattaag
      781 gagcaggtcc ccgtcgtagt ggcggaacag aagcccgaag cggccgccgt gattgcgcgc
      841 gtggcggcgg gaaagcatgc accgagtacc tgggtcggac gcgacattac gtttgacgaa
      901 acaggggaac cctgggcagt tcggttgaag gcacgatctg tcgacggggt ggctctgaag
      961 atgcagggcg tccatcaacg ccacaatttc gccaccgctc tggcggcact tgacgaaatc
     1021 ggttgtgtgc cgggtgaggc tgttgttcga aaggtagcgt ccgagacgat tttacccggg
     1081 cgcatgcagg tgctgccgac acgccctccc tgtatgctgg acgttgccca cacgcctgaa
     1141 tcggctgcgt gccttgcaga ggggattcgt cagacgttcg cgggcaagcg ggtgaccctg
     1201 gtcttcagtt gcgcatccga caaacaggtc gaaggaatcg cacaggtcct ggcgcccgtg
     1261 gccgggaacg tggttatcgt tcctatgtct ggacctagag cgatgtccat ggaaaggatg
     1321 tacgatgcgt ggtcgcgggc tcacacgcgt gtcgaacggg cagaaaccat ctcagctgct
     1381 gtggacagac tgaaggatgc cggatcagac agcgcgtggg ttgtatgcgg ctcgtttgtt
     1441 ctcgttggcg cggcaatgca gcttctcggc tattcgccac catagacggt atccttttac
     1501 ggtatcttgt gtgtaaatgt caaatgtttc caaactgaaa cgaaagcgag tcacaaatgt
     1561 cagaaaaact ggcgatccat ggcggaacac cggtccgaac gaaacccatg cctcccgcga
     1621 atcccggtga atgtatcatt ggagaagagg aaaaggagca ggtgctggag gtactcgacg
     1681 cccattcacc gttccgtttt tacgggcctg acctcaggaa gaaaacggag cagttcgaag
     1741 cggagttcga ggaattcatg ggcgtcaaac acgcgctcgg ggtcacctcc ggaaccgcag
     1801 cgctcaaagt tgcgctcgcg gcagccggcg tcgggcccgg cgatgaggtg atagtgccgg
     1861 cagtcacatt catagcgtgc gccggtgcgg ttgtcatgtc gagggcgcgg ccggtttttg
     1921 cgaatgtggg taccgatatc ggtctcgatc cggccgatgt ggaacgcaag atcacgaaga
     1981 gaactgcggc aataatgccg
//
LOCUS       DAOSOO010000367         2000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_56523_length_2000_cov_5.407712, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000367 DAOSOO010000000
VERSION     DAOSOO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56523_length_2000_cov_5.407712"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLT86_14980"
     CDS             <1..427
                     /locus_tag="PLT86_14980"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT37965.1"
                     /translation="HLIAEGHSQGGGQAVAAAALNRKVNGLVVSCCTHCDHTGPAVGR
                     VAGWPKIVNVENGVPDPVHLNAARYIDAVNFAARISCPTFWSMAFLDDLCPSVGNYAA
                     YNRLCSARKMVRHDVAVGHIHTDECKAATVKWVEEFVRS"
     gene            498..1505
                     /locus_tag="PLT86_14985"
     CDS             498..1505
                     /locus_tag="PLT86_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT37966.1"
                     /translation="MEPVKFAVVGLGMGHNRSRVIAGTEGAKLVTVVDIDEERARNVG
                     TELNVPWTLSLDEALNRFPEIECVVVMTPSGLHAEVGCQAAEAGKHVITTKPMDVSVE
                     ACNRLIKTCERKGVHLMVDFQSRYVDNNVRLAHAVQHGWLGRPLLAEFRFKWWRSQAY
                     YDERGGWRGTWKLDGGGSVANQGVHGLDLLLWVMGTPSAVYAESVVMNHKIETEDLSL
                     VIFDFPNGAKGAMTTTTTFPEASPYYSFEVHGTEGGVLLDDALGGVGRYFLKEGVQER
                     MQEITSPVKNVAEDAVRVIRRGARPVIDGREGRRTVHMLEMIYKSAREGRKIPVTPIT
                     D"
     gene            1727..>2000
                     /locus_tag="PLT86_14990"
     CDS             1727..>2000
                     /locus_tag="PLT86_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT37967.1"
                     /translation="MRVSLFITCLADLFHPEIGLAMVTILRKHGVELVFPGGQTCCGQ
                     PAFNTGNWAEARSVAARMLDVFESSEYVVSPSASCTAMIREGFPRLF"
BASE COUNT          393 a          624 c          598 g          385 t
ORIGIN      
        1 ccatctcatc gcagaggggc acagccaggg tggggggcag gccgtcgccg cggccgcgtt
       61 gaaccggaaa gtgaacggat tggtcgtctc atgctgcacg cattgcgatc acaccggccc
      121 tgcggtgggc cgggtagccg gctggccgaa aatcgtcaac gtcgagaacg gcgtacccga
      181 cccagtccac ctcaacgccg cgcgctacat tgacgcagtc aacttcgcgg cgcgcatttc
      241 gtgtccaacg ttctggagca tggccttcct cgatgatctt tgcccatccg tgggtaacta
      301 cgccgcgtac aaccggcttt gttctgcccg gaaaatggtg cgccacgacg tggccgtggg
      361 gcacattcat accgacgaat gcaaagcggc aacggtcaaa tgggtcgagg agttcgtgcg
      421 gagttgaaaa agtaatttcc ccggtaaccg tccgccattg cggagaacat gtccaagtgt
      481 tcagtcagga ggcaaccatg gaacccgtca aatttgccgt cgtcggtctc ggcatggggc
      541 acaatcgctc tcgtgtcatt gcgggcaccg aaggcgcgaa acttgtgacc gtcgtggata
      601 ttgacgaaga acgcgcgcgt aatgtcggca cggagctgaa cgtcccttgg acgttgtccc
      661 tcgacgaagc cctgaacagg ttccccgaaa tcgaatgcgt cgtcgtgatg actccaagcg
      721 gacttcacgc cgaggtcggt tgtcaggccg ccgaagccgg caagcacgtc atcaccacca
      781 agcccatgga cgtctcggtc gaagcctgca accgtctcat caaaacgtgt gagcgcaaag
      841 gcgtccacct catggtggat ttccagagcc gctacgtcga caacaacgtc cgactggccc
      901 atgccgtgca gcacggctgg ctggggcggc cgctcctcgc cgagttccgc ttcaaatggt
      961 ggcgctcgca ggcctactac gacgagcgcg gcggctggcg cggcacgtgg aaactcgatg
     1021 gtggcggttc cgtcgccaac cagggcgtgc acggcctcga tctcctgctg tgggttatgg
     1081 gaacgccctc cgccgtatac gccgagagcg tggtaatgaa ccacaaaatc gaaacggaag
     1141 acctgtctct cgtcatcttc gatttcccca acggcgccaa aggcgcaatg accaccacca
     1201 cgacgttccc cgaagcaagc ccgtactaca gctttgaggt ccacggcacc gaaggcgggg
     1261 tactcctgga cgacgcgctc ggcggcgtgg gaaggtattt ccttaaggaa ggcgtgcagg
     1321 agcgcatgca ggagataacc agccctgtca aaaacgtggc ggaagacgcc gtacgcgtga
     1381 tacgccgcgg tgcgaggccg gtcattgacg gccgcgaggg ccggcgcacc gtccacatgc
     1441 tcgaaatgat ctacaaatcc gcgcgcgaag gacggaaaat tccggtaacc cccatcactg
     1501 attgacctcg caaccggagt ggcgcggccg ttccgcgcca ctcccgccga tcctgccgtg
     1561 tgccctgcgg gagcgtccaa tctcgatttc cgcacccctt cgtccggatc cgctgcatta
     1621 tttcggcaca ggcatccctg cacgagtttc tgcttggccg gaatgcggct ttttggtatc
     1681 cttcttcact ctgtggtgaa cctcatttcg cgtaaaggca gtctcaatgc gcgtctcgct
     1741 gttcattacg tgcctcgccg acctgtttca cccggagata gggctcgcga tggtgaccat
     1801 tctgcggaaa cacggagtag aactggtgtt ccccggcggg cagacctgtt gcggccaacc
     1861 cgctttcaac accgggaact gggccgaggc ccgttccgtc gcagcccgca tgttggatgt
     1921 gttcgaaagc tcggaatacg tggtatcccc ttctgcgtcc tgcaccgcga tgattcgtga
     1981 gggatttccc cgcctgttcg
//
LOCUS       DAOSOO010000368         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_58885_length_1943_cov_4.186970, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000368 DAOSOO010000000
VERSION     DAOSOO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58885_length_1943_cov_4.186970"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLT86_14995"
     CDS             <1..180
                     /locus_tag="PLT86_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37968.1"
                     /translation="PENARHVTLSATPVGWSATGSTGRRRPGGFALATGGAIFSITPA
                     SRSEHLLAAWQDVRD"
     gene            297..1412
                     /locus_tag="PLT86_15000"
     CDS             297..1412
                     /locus_tag="PLT86_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37969.1"
                     /translation="MRISLNGQRIFPLGAYEHPRTEDEWRQWTKAGINIVCCHNRKEL
                     DAAHAHGVLGWVPVPVILADGDDGSTLAAKVNTLKDHPALAVWEAQDEAIWNASRKDD
                     NNVRMSFWKHPPEKLAEIEERFNAVVRGLERGAKVVREHDPGRPIWLNEAVNSDQETL
                     ARCVPYLDIVGFDWYPVRRNATVPMGLLGKQIQRFVRTAPLCGQWFVQQAFCWSQLKL
                     AEGPPLLPTEEQTRFMAWDAILHGATGIMWWGSAYADRPHPFFDGWMTVLREFEGLHP
                     FLFAGQMPHVRAETYYRQHDPILGVGVLARRAGNRTLVVLINQDQYAHETVLKGLDEA
                     VAMRLRRVGGGGEGLVKTREGFITALEGYDVRIYITD"
     gene            1425..1751
                     /locus_tag="PLT86_15005"
     CDS             1425..1751
                     /locus_tag="PLT86_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT37970.1"
                     /translation="MPIIQGPDAGVLTTEFAHWGLATFKAGMRNITRAHYHDCDEFVF
                     MIGGVMVMRSEGVEYTLRKDDVLVTRMGDEHEILEILEDTTYFWLETELRGRKRTGHL
                     HRGEDD"
     gene            1751..>1943
                     /locus_tag="PLT86_15010"
     CDS             1751..>1943
                     /locus_tag="PLT86_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37971.1"
                     /translation="MEGTLITPQELGVSAEFPLPKRSDWGIGMVGFGGIARTSHAPAY
                     KKAGWKIVAVADPDEKARTL"
BASE COUNT          380 a          577 c          649 g          337 t
ORIGIN      
        1 ccggagaatg cacgccacgt gacgctatcc gcaacgcccg tcggttggag cgcaaccggc
       61 agcaccgggc gccggcgccc gggtgggttc gccctcgcca ccgggggagc gatcttctca
      121 atcacgccag catcacgttc cgaacacctc ctcgccgcat ggcaggacgt gcgggattag
      181 ctgacatcgc gtccgatctc tgaaagggct gctgttcttt tgccgcgatt cgcgcatacc
      241 atcgcagggc ggggtggtcc cgcgtcgcga cacccacctt ctcgtggagg agtctcatgc
      301 gcatctctct gaacggccaa cgcatatttc cgctgggtgc ctacgagcat ccgcgaaccg
      361 aggacgaatg gcggcaatgg acgaaggccg ggatcaacat cgtctgctgc cacaaccgga
      421 aagagcttga cgcggcacac gcgcacggcg tgctgggatg ggtgcccgtg ccggtgattc
      481 tggccgatgg cgatgacggt tccacgcttg ccgcgaaggt gaacacgctg aaggaccacc
      541 ccgccctcgc cgtctgggaa gcccaggacg aagcaatctg gaacgcgtcc cgcaaggacg
      601 acaacaacgt gcggatgagc ttctggaagc acccgccgga gaaactggca gagatcgaag
      661 agcgtttcaa cgcggtggtt cgtgggttgg aacgcggtgc caaggtcgta cgggaacacg
      721 atcccggcag gccgatctgg ctcaacgagg cggtgaacag cgatcaggag acgctggcgc
      781 ggtgcgtgcc gtatctcgac atcgtcggct tcgactggta ccccgtccgg cgcaatgcca
      841 cggtgccgat gggccttctc ggcaagcaaa tccagcgatt cgtccgaacc gccccattat
      901 gcgggcagtg gttcgttcag caggcgttct gctggtcgca actcaagctg gcggaaggcc
      961 ctccacttct gccgacagag gaacagaccc ggttcatggc gtgggacgcg atcctccacg
     1021 gggccaccgg catcatgtgg tggggttcgg cctacgcgga caggccgcac ccgtttttcg
     1081 atggctggat gacggttctt cgtgagttcg aaggcctcca tcctttcctc tttgccgggc
     1141 agatgcccca cgtgcgggcg gaaacgtact accggcagca cgacccgatt ctgggtgtcg
     1201 gtgttcttgc gcgaagggcg ggcaaccgca ccctcgtggt actgatcaac caggaccagt
     1261 acgctcatga gacggtgttg aaggggctgg acgaagccgt tgcaatgcgc ctgcgacggg
     1321 tcggcggagg cggcgaaggc ctggtgaaaa cgcgcgaggg gttcatcact gcgctggaag
     1381 gctacgacgt gcgcatctac atcaccgact gaaaggagca cgcaatgccg atcattcagg
     1441 gacccgatgc gggtgtcctc acgactgagt tcgcccactg gggcctcgcc acgttcaaag
     1501 cggggatgcg gaacatcacc cgggcgcact atcacgattg cgacgagttt gtgttcatga
     1561 tcgggggcgt gatggtgatg cgctcggagg gcgttgagta caccctgcgg aaggacgacg
     1621 tgctggttac ccgcatgggc gacgaacacg agattctgga gatactcgag gacaccacgt
     1681 acttctggct cgagaccgaa cttcggggca gaaaacgcac cgggcacctg caccggggag
     1741 aggatgactg atggaaggca cactgattac gccgcaggaa ctcggcgtca gcgctgagtt
     1801 tccgcttccg aagcgatcag actggggtat cgggatggtg ggcttcgggg gcatcgcacg
     1861 gacgtctcat gcgccggcgt acaaaaaggc gggctggaag atcgtcgccg tggccgatcc
     1921 tgatgagaag gcacggacat tgg
//
LOCUS       DAOSOO010000369         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_58971_length_1941_cov_6.316543, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000369 DAOSOO010000000
VERSION     DAOSOO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58971_length_1941_cov_6.316543"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(586..1560)
                     /locus_tag="PLT86_15015"
     CDS             complement(586..1560)
                     /locus_tag="PLT86_15015"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOT37972.1"
                     /translation="MAITHVRFSGTRREIGRTQGYRIRALRLPPASPRDIVFGNRCRA
                     VAARLHPGIEDEFEGMCEASGLPVEAFTSYYFGRTAPAAGGCTNIAVTGARTSNGDTL
                     VGRNYDWDYADRRWCEARTISPEGEPARIGYTHHWGGLCDVMNDAGLTLCIASLPPEG
                     EPRPGIQWHIAVDLVAATCRTAPEAADLLASLPHVRSLAYLVADRATARLVETSPRNA
                     QVYEPDDGILVGTNTRTDALAAHAETASVRRRTRAENLLRAAKEPLTTDAVIRVLSDH
                     EGGICAGRHGPDAPGGGTIWSLVAEPTPRRARVAPGHPCDTPFEDILW"
     gene            1742..>1941
                     /locus_tag="PLT86_15020"
     CDS             1742..>1941
                     /locus_tag="PLT86_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37973.1"
                     /translation="MALNRRWATVFRVLLAACAVRAGAAARLERGVLLVDGKPFFPLG
                     SWGGREVAPEEIARLGMNATFL"
BASE COUNT          312 a          582 c          704 g          343 t
ORIGIN      
        1 atcggtggcc cgggcggcgc tgacaaggcc gcttgtggcg agggcgagca ggagggaaag
       61 aacaacgcga gaccgcagca tggcgcacct ccccgggtaa agccgatgcc ttgagcgcat
      121 atcacaatgc gttgaatgta gcccggtcag caccggttgt caacgatgag cggcgtgtcg
      181 atcgcacgga agtatagtca ttgggggcag gggggggcaa gtggcgaggg cggggaacag
      241 cttcgcagga cgattcgcgc tgggctggat tcccgcctgc gcgggaatga cgttcgcttc
      301 ttcgggatga ccagcataca cctgcgctgt cggaaatgac gaacggaaag tcattgactt
      361 tttctttgcc agagggtcag gaacatgccc acaatccgtc attcccgcgg aggcgggaat
      421 ccagaccggg tgcccgggcg cagctttcgc ggtcatctcg atacaccgtc cgccgaccgt
      481 ccggcggccg ccactcgatg accgctgttc gggcgcccac ttgccggtcg tcgagcggct
      541 cacgagcgca cgccagggag acgtatcgag acgcaccggc agtgtttacc agaggatgtc
      601 ctcaaacggg gtatcgcacg ggtggcccgg tgctacccgg gcgcgtcgtg gtgtaggctc
      661 ggcgacaagc gaccagatcg ttccgccgcc cggcgcgtcc gggccgtgcc gtccagcgca
      721 gatgccaccc tcgtgatcgg aaagaacgcg gatcacggca tccgttgtga gcggttcctt
      781 cgccgcgcgc agcaggttct ccgcgcgcgt tcttctccgg acggaggcgg tttctgcgtg
      841 tgccgcgagt gcgtcggtcc gcgtgttggt gcccaccagg atgccgtcgt ccggttcgta
      901 cacctgtgcg ttccgggggg aggtttcgac gaggcgcgcg gtggcgcggt cggcgacgag
      961 atacgcgaga gagcgcacgt gcggaagact ggcgaggagg tcggctgctt ccggggcggt
     1021 gcggcaggtg gcggcaacga ggtccacggc gatatgccac tggatgccgg gccggggctc
     1081 gccttccggt ggcaggctgg cgatgcagag cgtgagcccg gcgtcgttca tgacgtcgca
     1141 caggcctccc cagtggtgcg tgtacccaat gcgcgcgggt tcgccctccg gcgaaatagt
     1201 ccgcgcctcg caccagcggc gatctgcgta gtcccagtcg tagttccgtc cgacgagcgt
     1261 gtcaccgttg ctcgttctgg cgccggttac cgcgatgttg gtacagccgc cggcggcagg
     1321 cgcggttcgg ccgaagtagt agctggtgaa ggcctccacc ggcaagccgc tggcctcgca
     1381 catgccttcg aactcgtctt cgataccggg atgcaggcgc gcggcgacgg cgcggcaccg
     1441 gttgccgaac acaatatcgc ggggtgaggc gggcggcagc ctgagggcgc ggatacggta
     1501 cccctgcgtt ctgccgatct cccggcgcgt gccggagaaa cgcacgtgtg tgatcgccat
     1561 ggggtcgtct ccctgttgtc cgcggcggca catctgcgat tccgctcatc tgccgcgaat
     1621 gaaatagaac gggaattccg ttccgggaaa agaggaggga acggttgccc ccggaaccac
     1681 gccaccgtca gtttggttag ggctgcggcc ccatagtgaa ttcttcgcgg gaaggtgatg
     1741 aatggcgctg aacaggcgat gggcgacggt tttcagagtt cttctggccg cgtgcgcggt
     1801 ccgagcgggc gcggcggccc gccttgaacg cggtgtgctt ctcgtggacg ggaagccgtt
     1861 cttcccgctg ggaagctggg gcgggcggga ggttgcgccg gaggaaatcg cccggctggg
     1921 tatgaacgcc acgtttttga t
//
LOCUS       DAOSOO010000370         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_60562_length_1905_cov_2.714595, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000370 DAOSOO010000000
VERSION     DAOSOO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60562_length_1905_cov_2.714595"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLT86_15025"
     CDS             complement(<1..938)
                     /locus_tag="PLT86_15025"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOT37974.1"
                     /translation="MRRFGVLMLSACLACGVAYADIGEAPTNVFALITKPAGVALTNE
                     FDLNTAPPSIEEQPVSRTVNPGQSVTFTLTATGMPPLSYQWRKGGVDIADATDAIYTI
                     ASAQESDEGNYRCVVNNVAGSVSSDPATLTVNDPPVITAQPQSVTVNPGQNAVFSITA
                     NGTAPLSYQWKKGGVNIATGTGSSYTRSSAQQSDEDVYTCTVSNVAGSVTSAPATLTV
                     TDPPVFTAQPQSLTVNVGATASFGVTVTGTPPLSYQWRKGTADISGATNATFSIASTQ
                     TSDEGTYRCVVKNVANPTGAISDAATLAVNTPPQITA"
     gene            complement(1098..1358)
                     /locus_tag="PLT86_15030"
     CDS             complement(1098..1358)
                     /locus_tag="PLT86_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT37975.1"
                     /translation="MTLFDFAQWFLSRTNRFPKNGRKSLGDRIDASLLELLALVQRAS
                     LRRDKRALLDRVNEELHVLRALVRLAVRLDCLQERQYEYAAK"
     gene            complement(1490..>1905)
                     /locus_tag="PLT86_15035"
     CDS             complement(1490..>1905)
                     /locus_tag="PLT86_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT37976.1"
                     /translation="TFRLPTEAEWEYACRAGTTTRFYWGDDPSYSQIGTYAWYYDNSG
                     DTTHPVGQKTPNAWGLYDMSGNVWEWCQDWYGSYAAGAVSDPQGPSTGTFRVLRGGCW
                     NYVVRFCRSAPRLSYSPDSRSFVSGVRLCVSGSPR"
BASE COUNT          382 a          621 c          517 g          385 t
ORIGIN      
        1 gctgtgattt gaggtggtgt atttacggcc aatgtcgcag cgtcggagat cgcgccggtc
       61 ggattggcga cgttcttgac cacgcaccgg tacgtgccct catccgaggt ctgtgtggac
      121 gcgatgctaa acgtcgcgtt cgtcgcgccc gaaatgtccg ccgtcccctt ccgccactgg
      181 taactcagcg ggggcgtgcc cgtcaccgtc acgccgaaac tcgcggtggc gcccacattg
      241 accgtgaggg actgcggttg cgctgtaaac accggcgggt ccgtcaccgt caatgttgct
      301 ggagccgacg tgacgctccc cgcgacattg ctcaccgtgc aggtatacac gtcctcgtcc
      361 gactgctggg ccgaactgcg cgtgtacgaa ctgcccgtcc ccgtcgcgat attgaccccg
      421 ccctttttcc actggtaact caacggggcc gtaccattgg cggttatcga aaagaccgcg
      481 ttctgccccg ggttcaccgt aaccgactgc ggttgcgccg taatcaccgg cggatcgttc
      541 acggtcagcg tcgccggatc ggatgacacg ctgcccgcga cattgttcac cacgcaccga
      601 taattgcctt catcggactc ctgcgcggat gcaatggtat agatcgcgtc cgtcgcatcg
      661 gcgatgtcca ccccgccctt tcgccattgg taactcaacg gcggcatgcc cgtggccgtt
      721 agggtgaacg tgacgctttg tccgggattg accgtacggg acacgggctg ctcttcgatc
      781 gaaggaggcg ccgtgtttag atcgaattca tttgtcagcg caacgcccgc cggtttggtg
      841 atcaaggcga agacgttcgt cggcgcctcc ccgatgtctg cgtaggccac cccgcatgcg
      901 agacaggccg acagcatgag tactccaaac cgtctcatct tcattcctcc gtgtcaatat
      961 cttatccgct atctttgggt tcttttcaac cgccgaaata gcaaagaatg caaagcgtag
     1021 gacagactgt ccagtctgtc catcccaaaa ccatgccatg cccgcagatg gacgggctgg
     1081 acagcctgtc catcgatcta cttcgccgca tattcgtact gacgctcctg caagcaatcg
     1141 agacgcaccg ccagccgcac cagcgcccgc agtacatgca attcctcgtt cacgcggtcg
     1201 agcaatgccc gcttgtcgcg ccgcaggctg gcgcgttgca ccagcgccag caactccagc
     1261 aggctcgcat caatgcggtc gcccagcgat ttccgcccgt tcttcggaaa acgattcgta
     1321 cgcgacagga accactgcgc gaaatcgaac aacgtcaccg aaacatgagg ttcacatatg
     1381 ccagactgtt tcatttccgt gcacccattc cggcctccaa aaatccgcta ggggcttcgc
     1441 ccctagaacc ccataaaggc aaaaaaataa agtgtaaagg aaaaaccgac tagcggggcg
     1501 aaccggacac gcagaggcga accccgctga cgaagctcct gctgtcggga ctgtagctga
     1561 ggcgaggcgc ggaccggcag aacctgacga cgtagttcca acagccgccg cgcagcaccc
     1621 ggaacgtacc tgtactagga ccctgtggat ctgatactgc gcccgccgcg taactcccgt
     1681 accagtcctg gcaccattcc cagacgttcc cgctcatgtc gtaaagtccc caggcgttcg
     1741 gcgtcttctg gcccaccgga tgcgtcgtat cgccactgtt gtcataatac catgcatagg
     1801 ttccaatctg gctgtaactt ggatcatcgc cccagtaaaa ccgagtggtg gttcccgccc
     1861 ggcaggcgta ttcccattcg gcttccgtag gcaaccggaa cgtca
//
LOCUS       DAOSOO010000371         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_61165_length_1892_cov_4.211758, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000371 DAOSOO010000000
VERSION     DAOSOO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61165_length_1892_cov_4.211758"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLT86_15040"
     CDS             <1..1045
                     /locus_tag="PLT86_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT37977.1"
                     /translation="RDDVRHMSQLLKAAGYTTHLFGVQHEVVPDELPRLAFDSIRGQG
                     FPSAKVIAADVADLLRSRGKEPAPFYAQVGFFQTHTPFDHGGVKPDDERGVEVPPYLA
                     DCESSRKAMAAFQGAIRHVDEAVGIIMEALHESGLDDDTIVVFTTDHGIEMPRAKWFL
                     YDPGIAIACIVRYPGAGATGGTTCDLLLSNVDYLPTLFDLAGIPVPGGLHGRSFAPSL
                     RGECSEPVREAMFALYHKSQSRCVRTSRYSLIRHFDAPIDFATVPVRFEEVLAKRGIP
                     QVELYDLEADPNQFENVASKPEYADIKVRLDTMLWEWMESVNDPLLKGPVRSPSYESA
                     MADYGAWKGNAHT"
     gene            1107..1688
                     /locus_tag="PLT86_15045"
     CDS             1107..1688
                     /locus_tag="PLT86_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37978.1"
                     /translation="MPSSTLELDFKGPYRFVGRDRSVFEAPCAASPGVYLWTVKQHDG
                     THMIHYIGQTSAFAKRQREHLIQVLGLNYGIFNAEKAQTGVSELIWPGMCRKLPEEGP
                     LQAVEEYKRINEKVLEYLSVLNVFFAEADVGRSLLRHIEGCIGWNLRNNHPESKALFP
                     DGVRVGTLMQKDHGLLRIRSTEPIRGLDAEIPY"
BASE COUNT          375 a          546 c          599 g          372 t
ORIGIN      
        1 ccgagatgac gtgcgtcaca tgagtcagtt gctcaaggcg gcaggctaca caacgcacct
       61 gttcggcgtc cagcatgaag tcgttccgga cgaactgccc cgtctggcgt tcgactccat
      121 ccgggggcaa ggtttcccgt ccgcgaaggt aattgccgcc gacgtggcgg atttactgcg
      181 ttctcgagga aaggaaccgg cgccgttcta tgcgcaggtc gggtttttcc agacccacac
      241 cccgtttgat cacggcgggg tgaagcctga tgacgagcgc ggggtggaag taccaccgta
      301 tcttgccgat tgtgaatcgt cgaggaaggc catggcggcc tttcagggcg caatccgcca
      361 cgtggatgag gcggtcggca tcatcatgga agcactgcac gagagtggcc tcgacgacga
      421 taccattgtg gtttttacca ccgaccacgg gatcgagatg ccgcgcgcca agtggtttct
      481 gtacgatccc ggcatcgcca tcgcgtgcat cgtgcggtac ccgggggccg gcgcgaccgg
      541 cggcacaacg tgtgatctac tcctcagcaa tgtggattac ctccccacac tgttcgatct
      601 ggcgggaata ccggttccgg gcggcctcca cgggaggagt ttcgccccca gtttgcgagg
      661 cgaatgttcc gaaccggtgc gcgaggccat gttcgccctc taccacaagt cgcagtcccg
      721 gtgtgtgcgg accagtcgct actcgctgat ccgccatttc gacgccccga tcgatttcgc
      781 caccgtaccg gtgcgtttcg aagaggtgct ggcgaagcgg ggcataccac aggtggagct
      841 gtacgatctg gaggccgatc cgaaccagtt tgaaaacgtc gcttcaaagc ccgaatacgc
      901 ggatatcaag gtgcgcctcg acaccatgct gtgggagtgg atggagagcg tgaacgaccc
      961 gctcctgaaa gggcctgtgc gttccccgtc atatgagtcc gcgatggcgg actatggcgc
     1021 ttggaagggc aacgcgcaca cctgatggcg gggcggcttt tggaggggcg cgttccgtcg
     1081 cgcccgtcgg gaagggaacc acataaatgc cgtcatctac gttagaactg gacttcaagg
     1141 gcccctatcg cttcgtcggt cgggatcggt cggttttcga ggctccttgc gccgcgtctc
     1201 ccggcgtgta cctgtggacc gtcaaacagc acgacggcac gcacatgatc cattacatcg
     1261 gccagacgag cgcgttcgca aaacgccagc gtgagcatct cattcaggtc ctggggctca
     1321 attacggcat attcaacgcc gaaaaggcgc aaacgggtgt gagcgaactc atctggcctg
     1381 gaatgtgtag gaagctgccc gaagaagggc ccttgcaggc tgtggaggag tacaagagga
     1441 tcaacgaaaa agtactcgag tacctctccg tcctcaacgt tttttttgcg gaagcggatg
     1501 ttggacggtc gctgctcagg cacattgagg gttgcatagg gtggaacctt cgaaacaacc
     1561 acccggagtc gaaggctctg tttccggatg gtgtccgggt aggaacgttg atgcaaaaag
     1621 atcacggtct gctgcgcatt cgctccacgg aaccgattcg ggggctggat gcggagattc
     1681 cttattgact tgaagcctcc atgagcggcg cctgtgcgtt tcagacaagc ggaatcgggg
     1741 gttcaccggc aaaggagagg accatggccc atgaatgcgg tcaaaagacc atcaacctcc
     1801 ccgcccaaca ggttcccgtc atggcggacg ccgacgtggt cgtggtgggc ggtggcacct
     1861 ccggcttcat cgccgccgtc gccgcagcgc gg
//
LOCUS       DAOSOO010000372         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_61720_length_1880_cov_4.974795, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000372 DAOSOO010000000
VERSION     DAOSOO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61720_length_1880_cov_4.974795"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLT86_15050"
     CDS             complement(<1..490)
                     /locus_tag="PLT86_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37979.1"
                     /translation="MAGPRETGGKVLWSGRVVAVQPRIRLTRSFDERSHSYLGYVLRI
                     DGTCSGEAGEFLIAVGEGAHEKHRFQTGMELVGQSVPVEDPRKETAALYKTSGLKVVK
                     NAEGETPAGPPFIWVPPDLETYRARGHRRLDARTYDTKCTTCIWGCRMPVEMIIDQWN
                     PSK"
     gene            complement(490..828)
                     /locus_tag="PLT86_15055"
     CDS             complement(490..828)
                     /locus_tag="PLT86_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37980.1"
                     /translation="MIQDLKQIEYRRGMLEKGMKPEDLPVKVWQVAEIPADVRFAINA
                     ENLLNLGGVYGDKKAGDPMEYDNLKLVLTDDTVEITVFNRGITLFMSNDERVRRIHRV
                     LCKLAGSGRE"
     gene            complement(825..>1880)
                     /locus_tag="PLT86_15060"
     CDS             complement(825..>1880)
                     /locus_tag="PLT86_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT37981.1"
                     /translation="RQSSEVQSWLARIVRRLSDIESPFNREILSLLKTAMDKPHYTIL
                     RGYREQLLGVLMAYFRSPAFVARYLPLDDPLVRKALGERETRRDIIARGIDALRHAIL
                     EEKDASNQTYLGRVGQFLDFASELAERAERRLVLKEDEKSDESENPLLEYLDAVSIYS
                     KPRRLDHADDDGVEDETDDGSYRVMPLVRMVYGETKTEIRDRLMLAFNSPLFPEILVS
                     SSVMGEGVDLHRFCRHIIHHDLCWNPSTLEQRTGRLDRVRCKAEVCSRSIEVYEPFLA
                     GSADEKMFRVLRDRERWFQIVMGQKFEFDEATSEAIACRLPLPSELARALTFSLSCWP
                     HRPYAPGARHPDEGVDR"
BASE COUNT          385 a          577 c          508 g          410 t
ORIGIN      
        1 tctttgacgg attccactgg tcgatgatca tctccaccgg catccggcag ccccaaatgc
       61 aggtcgtgca tttggtgtcg taggtccggg cgtccaggcg gcgatgtccc cgagcccggt
      121 aggtttccag gtcaggcggc acccaaatga aaggcggccc ggcgggcgtt tcaccctcgg
      181 cattcttgac gaccttgagt ccgctcgtct tgtagagcgc cgccgtctcc ttgcgcgggt
      241 cttcgaccgg aaccgattgt ccgacgagct ccatgcctgt ctgaaaccgg tgcttttcgt
      301 gcgcaccctc accgaccgcg atcaggaact ctccggcctc cccactgcag gttccgtcga
      361 ttcgaagcac atacccgagg tagctgtggg accgctcatc aaaggaacgc gtaagccgta
      421 tgcggggctg gacggccaca acacggccgg accatagcac cttgcctccg gtttctcgtg
      481 ggccggccat cactctctcc ccgatccagc gagtttgcac aggacccgat gtatacgccg
      541 aaccctctcg tcgtttgaca tgaacaacgt gatccctcga ttgaaaaccg tgatctcgac
      601 ggtatcgtcg gtcaagacca gcttgaggtt atcatactcc atcggatcgc cggccttctt
      661 gtcaccgtaa acgccgccga ggttcagcag gttctctgcg tttatcgcga agcgcacatc
      721 cgcaggaatc tcggccacct gccagacttt gacgggcaga tcttcaggct tcatgccctt
      781 ttcaagcata ccccggcggt attcgatctg tttgaggtct tggatcatcg gtccacccct
      841 tcatctgggt gcctcgcgcc cggtgcgtat ggtctgtgag gccagcacga aagactgaat
      901 gtaagcgctc gggccagttc gcttggcagt gggagtctgc atgcaatggc ttcagaggtt
      961 gcctcgtcaa actcaaactt ctggcccatc acaatctgaa accagcgttc ccggtcacgc
     1021 agcacccgaa acattttctc gtccgcgctg cctgcgagaa acggttcgta cacctcaatt
     1081 gaccgggaac acacctccgc cttgcagcga acacggtcaa gacgcccggt tcgttgctcc
     1141 agagtgctcg gattccagca gagatcatgg tgaataatat gacggcagaa ccgatgcagg
     1201 tccacacctt cgcccatgac cgaactgctc acaagaattt ctggaaataa ggggctgttg
     1261 aatgccagca tcaggcggtc cctgatttcc gttttcgtct cgccatagac catgcggacc
     1321 aaaggcatca cgcggtaact gccgtcgtcc gtttcatcct caacgccgtc atcatcggca
     1381 tggtccaacc gccttggttt ggagtagata ctgacggcat cgagatactc caacaaggga
     1441 ttttccgatt cgtcggattt ctcgtcttcc ttcagcacca aacggcgttc ggcacgttcc
     1501 gcgagttcgc tggcaaaatc aaggaattgg ccgacccgcc caagataggt ctggtttgac
     1561 gcgtcttttt cctcaaggat tgcgtgccgc agagcatcta ttcccctggc aatgatgtcg
     1621 cggcgggtct cgcgctcgcc caacgcttta cgcacgaggg gatcgtcgag gggaaggtag
     1681 cgggcaacga acgccggaga ccggaaatac gccatcagaa ctccaagaag ttgctcccga
     1741 tagccccgga ggattgtgta gtgcggcttg tccatcgcgg tcttcaatag cgagagaatc
     1801 tctcgattga aggggctctc aatatcggag agacgacgaa caatccgggc aagccagctc
     1861 tgaacctctg aagactgccg
//
LOCUS       DAOSOO010000373         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_61774_length_1879_cov_6.398026, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000373 DAOSOO010000000
VERSION     DAOSOO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61774_length_1879_cov_6.398026"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="aroC"
                     /locus_tag="PLT86_15065"
     CDS             <1..670
                     /gene="aroC"
                     /locus_tag="PLT86_15065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT37982.1"
                     /translation="LGYEGVSVLGYTCQVGDVKMPTMERMTLEQVEASPVRCPDPETA
                     AAMENLIKQVRSERDSIGSAVEIVARGVPAGLGEPVFDKLKADLAKALVSVPAVVGFE
                     YGSGFAAVPSRGTQHNDPIIRRQTGSGGAPVIGTRTNNAGGMLGGISTGEPIVVRVAL
                     KPTASIPQPMETVDTQGNPQTIVTKGRHDPCLAPRFVPIGEAMVALVLADHLLRQRAI
                     RGTE"
     gene            732..1481
                     /locus_tag="PLT86_15070"
     CDS             732..1481
                     /locus_tag="PLT86_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT37983.1"
                     /translation="MQPIRFHEMRPSQIKPIREKFPVCYVPMGVLEWHGEHNPVGLDA
                     IKATAMAEYFARHIGGLVMPTVWWGDNRAILAENHFGPEICAEIAKGHGLPMQAFFDD
                     AERAKQDGEWKNWEAVMRNTFYELTTFGFKVIVTISGHYPLHGPAINAANAFEPTGRA
                     KIISIIGFDLVKDRFEGDHAAKWETSLMLYLRPDLVDMSALDPDPAKKPVGVLGVDPR
                     GNASAEYGKQGMEAMTEALRPKVLEALAAMQ"
BASE COUNT          400 a          553 c          584 g          342 t
ORIGIN      
        1 gctggggtac gagggtgttt ccgtgctcgg gtacacctgc caggtcggcg acgtgaagat
       61 gcccacgatg gaacggatga cactggaaca ggtcgaagcg agccccgtcc ggtgcccgga
      121 tcccgaaacc gccgcggcga tggagaacct gatcaaacag gtgcgttcgg agcgggattc
      181 catcgggagc gcggtggaaa tagtcgcccg gggcgttcca gcggggcttg gtgagccggt
      241 attcgacaag ctcaaagccg atctcgcgaa ggcgctcgta agcgtgccgg cagtggtggg
      301 ctttgagtac ggttcgggct ttgccgccgt gccctcacgg ggcacgcagc ataacgaccc
      361 gatcatccgg cggcagacgg gttccggcgg ggcaccggtg atcggcacaa gaaccaacaa
      421 cgcgggtggc atgctcggcg gaataagcac cggggaaccg attgtcgttc gcgtggctct
      481 caagccgacg gcaagcatcc cgcaacccat ggaaaccgtg gacacgcagg ggaacccgca
      541 gacaatcgtt accaaagggc ggcacgatcc gtgcctcgca ccgcgctttg ttccgatcgg
      601 agaggccatg gtcgcgctgg tgctggccga ccacctgctt cgccagcggg cgatacgcgg
      661 cacggagtag acgaaagcca ccgcagaaga atcacttgat gcaccacacc gcacatccaa
      721 aggacatgac gatgcaaccg atccgattcc acgaaatgcg gccgagccag atcaaaccga
      781 tccgcgagaa gttccccgtc tgttacgtgc ccatgggtgt gctggaatgg cacggagagc
      841 acaaccccgt cggtctcgac gccatcaaag ccactgccat ggcggagtac ttcgcgcgcc
      901 acatcggcgg tctcgtcatg ccgacggtct ggtggggaga taaccgggca atcctggcgg
      961 agaaccactt cgggccggag atttgcgcag aaattgccaa agggcacggg cttcccatgc
     1021 aggcgttctt cgacgatgcc gagcgcgcaa aacaggacgg cgaatggaag aactgggaag
     1081 ccgtcatgcg gaacactttc tacgagttga cgaccttcgg cttcaaggtg atagtgacga
     1141 tttccgggca ctacccgctc cacgggccgg cgatcaacgc ggccaacgcg ttcgaaccca
     1201 ccggcagggc gaaaatcatc tccatcatcg ggtttgatct ggtgaaagac cgcttcgaag
     1261 gcgaccacgc ggcgaaatgg gaaacctcac tcatgctcta cctgcggccc gacctggtgg
     1321 acatgagcgc gctcgacccc gaccccgcga agaagccggt gggcgttctg ggcgtggatc
     1381 cgcgtggcaa tgcctccgcg gaatacggaa aacagggcat ggaagccatg acagaggccc
     1441 tgcgcccgaa ggtgcttgaa gcactggcgg cgatgcaatg agggagcgat gccaccgctc
     1501 gacggtacaa ttcttgttac cactgagttg acagcttttt gaagcagcat atttttgtaa
     1561 ttgcaggcga gtacattcgg cgcgcacaga taagtctttt ttccacgacg gtgtaacgcg
     1621 ttccgcctgt ctccttttgc gctcgggatc agttttggtg aaaaattggt gataaaccgg
     1681 tcccattcgg gccggaacag ggtgtactgc gcgggagtat ctggcttctc ccgcactgtt
     1741 cttgcacgat ttttctgcgc gtacgcgtga agggaggcca ccatgcgttt acttctttcg
     1801 ttcctcaccg tagcattgtt cgccccggcg gcacactctg ccaacgtttc tggcactatc
     1861 tcgcaaaaca ccacatgga
//
LOCUS       DAOSOO010000374         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_62024_length_1874_cov_3.987905, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000374 DAOSOO010000000
VERSION     DAOSOO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62024_length_1874_cov_3.987905"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLT86_15075"
     CDS             <1..269
                     /locus_tag="PLT86_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37984.1"
                     /translation="HGNYLLCCQDNTGETAGMFGNVRDGFDGFLRFWFGQTMQQYRRN
                     LIAKNRAANDQCSRCNITFSRCDYLHWTDEQMSKYWDGSQWLPM"
     gene            254..703
                     /locus_tag="PLT86_15080"
     CDS             254..703
                     /locus_tag="PLT86_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37985.1"
                     /translation="MVTDVKARRPVGRPRKNINPEIVEALARIQCTDSEIAAVLDVNE
                     RTIQRNFAAILKKGREAGKQSLKRMQYKLAEEGNATMQIWLGKQYLGQKDKVETEHTG
                     KGGGPIAVNVSHRVTSFDWESFRQELEYQKQRAVESEGGGDTQGDGA"
     gene            690..>1874
                     /locus_tag="PLT86_15085"
     CDS             690..>1874
                     /locus_tag="PLT86_15085"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HOT37986.1"
                     /translation="MTVLENLYIKHVPFARQAAFLVLPHEEILYGGAAGGGKSDALLM
                     AALQFVHVPGYAAILFRRTYADLALPGALMDRAHDWLDGTDAHWNETQKTWTFPTGAS
                     LSFGYLDHEDTRYRYQSAEFQFVGFDELTQFSETQYTYLFSRLRRLEGCDVPIRMRAG
                     TNPGGPGHDWVNRRFGCDGKTPRPATRFFVPAVAAENIYLDREAYATALANLDPITRA
                     QLANGDWSTLASPGALWKYEWIGDSRLPMEKREEILKTAKRIVVGIDPAVTSTEHSDE
                     NGIVVCARGADNRGYVIADRSMRGSPLAWASRAIGAYEEFGADRIIAETNQGGEMVLH
                     TLRTVRRDVVVDGIVARRGKALRADPIASMYEQGLISHVGSFPELEGQMLSWVPGEGD
                     SPD"
BASE COUNT          415 a          512 c          584 g          363 t
ORIGIN      
        1 cccacggaaa ctacctgcta tgctgccagg acaacacggg agaaaccgct gggatgttcg
       61 ggaacgtacg tgatgggttc gacggatttc tgcggttctg gtttggacag actatgcagc
      121 aataccgccg gaaccttata gcgaaaaacc gagcggcgaa tgatcaatgc tcgcggtgta
      181 atatcacctt ttcgcgctgt gactacctgc actggacgga tgagcaaatg agtaaatact
      241 gggacggcag tcaatggtta ccgatgtaaa agcccgccgc cctgttggtc gcccgcgcaa
      301 gaacataaac ccggaaatcg tcgaggcgct tgcccggatc cagtgcaccg actcggaaat
      361 cgcggcggtg ctggacgtta acgaacgcac gatacagcgt aattttgccg ccatccttaa
      421 aaagggccgt gaagccggca agcagtcgct caagcggatg cagtacaagc tggccgagga
      481 aggcaacgcg acgatgcaga tatggcttgg gaagcagtac ctcgggcaga aggacaaggt
      541 ggagaccgaa cacactggta agggcggcgg cccgatagcg gtgaacgtct cgcaccgcgt
      601 gacgtcattc gattgggaat cgttcagaca ggagcttgag tatcaaaaac agcgtgctgt
      661 ggagtcagag ggcggaggag acactcaggg tgacggtgct tgagaacctg tacatcaagc
      721 acgtgccgtt cgcacgtcag gccgccttcc tcgtgctgcc acacgaggag attctttacg
      781 gcggtgccgc cggcggtgga aagtctgacg ccctgctcat ggctgcgctc cagttcgtgc
      841 atgtgccagg atacgcggca attcttttcc gccgtacgta tgccgatctg gccttgcccg
      901 gcgcgttgat ggacagggca cacgactggc ttgatggaac cgatgctcac tggaatgaaa
      961 cacaaaagac atggacgttt ccgacaggag catcgttgtc attcggatac ctggaccacg
     1021 aggatacacg gtatcggtac cagagcgccg agtttcagtt cgtcggattc gacgagttga
     1081 cccagttctc cgagacacag tatacctacc tgttttctcg tctgcgtcgg ctggaaggtt
     1141 gtgacgtgcc gattcgcatg cgtgcaggaa ctaaccctgg cggaccgggg cacgactggg
     1201 taaacagacg gttcggttgc gacggaaaaa cgccgcgtcc ggctacgcgc ttctttgttc
     1261 cggcggtggc ggcggagaat atctatctcg acagggaagc ctatgcaacc gccctcgcca
     1321 accttgaccc gataacccgc gcgcagcttg cgaacggcga ctggtcaacg cttgccagcc
     1381 ccggcgcgct ctggaagtac gaatggattg gtgacagtcg gcttccgatg gaaaaacgcg
     1441 aggaaatcct gaagaccgcg aagcgcatcg tcgtcggtat tgaccctgct gtcaccagca
     1501 cggaacattc cgacgagaat ggcatcgtcg tgtgcgcgcg cggtgctgac aatcgaggct
     1561 acgtcatcgc agaccgttcc atgcgcggct ctccacttgc atgggcgtcg cgcgcgatcg
     1621 gtgcgtacga agagttcggg gcggatcgca tcatcgcgga aacgaaccag ggcggtgaaa
     1681 tggtgcttca taccctgcgc acggtgcggc gtgacgtagt cgttgatggt atcgtcgctc
     1741 ggcgtggcaa ggcattgcgt gcggatccga ttgcgagcat gtacgagcag ggcctgatct
     1801 cacacgtcgg ttctttccca gaactcgagg ggcaaatgct ctcatgggtt ccaggcgaag
     1861 gcgacagtcc cgac
//
LOCUS       DAOSOO010000375         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_62578_length_1861_cov_8.127353, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000375 DAOSOO010000000
VERSION     DAOSOO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62578_length_1861_cov_8.127353"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1159
                     /locus_tag="PLT86_15090"
     CDS             107..1159
                     /locus_tag="PLT86_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT37987.1"
                     /translation="MKKRNTVGLDMGDKIHSLCVLDWRGQVVERAEVGNTGAAIRKWF
                     GKMKPCRVAMEAGTHSGWVSRILEELGHEVLVGNPRKLRAIWDNAQKNDVRDAEMLAR
                     IARFDPQLLHPIRHRGKEAQCDLAAIKARDELVKVRTALIAHVRGLVKSTGERLKKCS
                     PECFHLRAAEAMPADLAPALEPVVASIAELTVRIRHCDNLIEQLSADKYPETERLQAI
                     PGVGPVTALAYVLTLEEAERFEKSRDVGPFLGMTPRRDQSGQTDKQLRITKAGNAYLR
                     RLLVQCANYVLGRHGPDCDLRRFGLKLAERGGKNAKRRAVVAVARKLGVLLHSLWKSG
                     EAYEPNRRPVRAQKVA"
     gene            1574..>1861
                     /locus_tag="PLT86_15095"
     CDS             1574..>1861
                     /locus_tag="PLT86_15095"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOT37988.1"
                     /translation="MTATEIVRRCDDLLRGEESYCKLTMTVTRPKWSRTVSMEAWTRG
                     TKESFIRVLSPPKEQGVTFLKKGREAWQYAPAIDRVIKIPPSMMLQSWLGSD"
BASE COUNT          397 a          488 c          649 g          327 t
ORIGIN      
        1 ttccatagat aggatgagaa ggcaaaaagg aggatcggtt tcccttcgga tgagataaaa
       61 ctatctcagt ttgcgcactc gataaggtgc agaaaggaaa ccgaccatga agaagcgtaa
      121 tacagtgggc ctggatatgg gagacaaaat tcattcgcta tgcgtcctgg attggcgggg
      181 gcaggtggtg gagcgtgcgg aggtgggcaa cacaggtgcg gcgatccgca agtggtttgg
      241 gaagatgaag ccctgccggg tcgccatgga ggcgggaacg cattcgggct gggtgagccg
      301 gatcctcgag gaactgggcc acgaagtgct ggtgggcaat ccgcgcaagc tgcgggcgat
      361 ttgggacaac gcgcagaaga acgacgtgcg cgatgcggag atgctcgcgc gaatagcccg
      421 gttcgatcca cagttgcttc atcctatccg ccaccggggc aaggaggccc agtgcgatct
      481 ggccgcgatc aaggcccggg acgagctggt gaaagtccga acggcgctga tcgcgcacgt
      541 gcgcgggttg gtgaagtcga ccggggaacg cctgaagaag tgcagcccgg agtgttttca
      601 tctgcgcgcg gccgaagcga tgccggcgga tctggctccg gcgctggagc cggtggtggc
      661 gagcattgcg gaactgacgg ttcggattcg ccattgcgac aatctgatcg aacaactttc
      721 ggcggataaa tatccggaaa cggagcggtt gcaggccatc cccggcgtgg gtcccgtgac
      781 ggcgctggcc tatgtgctga cgctggaaga agcggaacgc ttcgaaaaga gtcgagacgt
      841 aggccccttc ctgggcatga cgccgcgccg ggaccagtcg ggtcagaccg acaagcagtt
      901 gcgcatcacc aaggctggca atgcctacct tcgacgattg ctggtccagt gcgccaacta
      961 cgttctcggg cgccacggcc ccgactgcga tttgaggcgg tttggcctga agctggccga
     1021 gcgtggcgga aagaacgcca agcgccgggc ggtagtggcc gttgcccgca agttgggcgt
     1081 actgttgcac agcctttgga aaagcggcga ggcctacgag ccgaaccgcc gccccgtccg
     1141 ggcgcagaaa gtagcttaga aaaggaagag agggaaaccc tccgttggtg gttcgtttgt
     1201 tcaaacaagg gagacgaatg acagacaacc ccgtttgaca gttctttgac gtcccgtccg
     1261 ggcgactgcg cttacacgct gggactgatg ctgcgctagg catcgtcaaa agtccgatcc
     1321 atagattgca gcgccccgcg acggacccta ccatgcaccg gagcatagcg gctccaccac
     1381 gagtgcgaat aagagcgtga acgcaaagaa ccggccatgc ggtgtccgtc aaaacggacc
     1441 tcttgacttg ccttctcata agagcgtgga aaactcgggt agtctgtgcg ccatgaagag
     1501 gcgcggcatt aggcggcgac taattgggct tttggtggcg gggctggttt gcgcctccgc
     1561 gcggggggag gaattgacgg cgacggagat cgtgcgacga tgtgacgatc tgctgcgggg
     1621 ggaggagagc tactgtaagc tgacgatgac ggtcacgcgc ccgaaatggt cgagaacggt
     1681 ctcgatggag gcgtggacgc gcggcaccaa ggagtcgttc attcgcgtgc tctcgccgcc
     1741 gaaagagcag ggtgtgacgt tcctgaagaa ggggcgcgag gcgtggcaat acgcgccggc
     1801 catcgaccgc gtcatcaaga ttccgccgtc gatgatgttg cagtcgtggc tgggcagcga
     1861 t
//
LOCUS       DAOSOO010000376         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_62615_length_1860_cov_6.777839, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000376 DAOSOO010000000
VERSION     DAOSOO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62615_length_1860_cov_6.777839"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLT86_15100"
     CDS             complement(<1..436)
                     /locus_tag="PLT86_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT37989.1"
                     /translation="MSSHFDGEKFRNQVPVPNHGFGDLIRWRFSREPGEWNDSNHLAP
                     GPAPPERVGNGNLRVTFVNHSTVLIQMDSLNILTDPIWSDRAGPFSWAGPKRVRPPGI
                     RFEDLPPIDAIILSHNHYDHTDIPTLKRLFERDAPRVFVALGN"
     gene            complement(610..>1860)
                     /locus_tag="PLT86_15105"
     CDS             complement(610..>1860)
                     /locus_tag="PLT86_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37990.1"
                     /translation="LIAWLFRVTGTSVFAARLAMLGCVAASAWLMTGAARSAGIRPLW
                     RAGAVALFLLLVPAFYSSHALTEVPVMVCSIGLAALLLRGEAPHPVGTWVSAGLIIGV
                     AASAKQVGVFLLPAAVIWIWVENRRSGVDVRSLIVRAAALSVAFAIPLAAWALYYLVI
                     GAFPEFLYAAWSVAASYRGFGFVDWVLNTARSVVLRGLIIWVPLAAGVPVILWRYLRR
                     SEYRVEALLSLIVVFSLIPILKRPYEHYITAFLPVACLLAVCVVRDSLRRYSGRQLIA
                     ALAILSLGTVRHYATYVIPPLVRGEYFQQKAVGERIASYLLPGEPLYVLGGEPKYYLL
                     SGHFHTDEVVFVIKDWEHLRPPQTVWDIIQNTPRLYHVLVVQGDLSVVGPVVEVVREE
                     GQHLFTRRVGELETVSLYRIHSTP"
BASE COUNT          421 a          588 c          569 g          282 t
ORIGIN      
        1 tgttgcccag tgccacaaaa acgcgaggag cgtcgcgctc gaagagccgt ttgagtgtcg
       61 ggatatcggt atggtcgtaa tggttgtggc tgaggataat tgcgtcgatg ggcggcaaat
      121 cctcgaaccg gatacccggc ggccgcactc gtttcggtcc cgcccacgag aagggcccgg
      181 cacggtcgga ccagatgggg tcagtcagga tgttcaggct gtccatctgg atgaggaccg
      241 tggaatggtt gacgaaggtc acgcgcaggt taccgttccc gacgcgttcc gggggagcgg
      301 gccccggcgc aaggtggttt gaatcgttcc actcgccggg ctcccggctg aatcgccagc
      361 gaatcagatc gccgaacccg tggttcggca ccggtacctg gttccggaat ttctcaccat
      421 cgaaatggga tgacatgggg cctcggtacg acggggcgga aagagcgcac cccgccagaa
      481 agaaggcgaa aaggaatggc aaaaatgaaa agcggatcat gcgacaccat ctgtctcgac
      541 gtgaacaaga ctgctcagtg tcgtagaaac gctcacaccg tgcagattgg ttcccgtgcc
      601 cggcgacctt tacggcgtgc tgtgaatccg gtacagcgac acggtctcca gttctccgac
      661 tcgccgggta aacagatgct gcccttcctc ccgcacgacc tccaccaccg gaccgacaac
      721 gctcagatcg ccctgcacaa caagtacgtg gtacaacctg ggagtgttct ggatgatatc
      781 ccacaccgtt tgaggcggcc tcaggtgttc ccagtccttg atgacgaaca ccacctcatc
      841 cgtgtgaaag tggccgctca gcaggtagta cttcggttct ccgccgagga cgtacaacgg
      901 ctcaccgggg agcaggtagc tcgcgattcg ttctccgacc gccttttgct ggaaatactc
      961 cccgcggacc agcggcggga tgacgtacgt ggcatagtgg cgcaccgtcc caagcgacag
     1021 aatcgccagc gcggcgatca gctggcggcc tgaatatcga cggagtgaat ccctcaccac
     1081 acacaccgca agaagacagg caacgggcag gaatgcggtg atgtagtgct cgtacggccg
     1141 cttcagaatc gggataaggg aaaacaccac aatcagcgag aggagcgcct cgacccggta
     1201 ttcactcctc cgcaggtagc gccagagaat aaccggcacc cccgccgcca gcggcaccca
     1261 gatgatcagt ccccgcagca cgacgctgcg cgcggtgttg agcacccagt caacgaagcc
     1321 gaagccgcga taggaggccg caaccgacca ggcagcgtag aggaactccg ggaatgcgcc
     1381 aataacgagg tagtacagag cccacgcggc gaggggtatt gcaaaggcca cagagagcgc
     1441 cgccgcccga acgatgagcg acctcacatc aacaccgctc cgccggttct ccacccagat
     1501 ccagatgacc gccgccggga gcaggaacac gcccacctgc ttcgccgaag ctgcaacgcc
     1561 gatgatcagg ccggcgctca cccacgtacc aacggggtgt ggagcttcgc cgcgaagcag
     1621 aagcgccgcc aacccgatgc tgcacaccat caccggcact tccgtcagcg catgggagga
     1681 atagaatgcc ggcacaagga ggaggaagag ggctactgcc ccggctcgcc agagcgggcg
     1741 aattccggcg ctgcgcgcgg cacccgtcat gagccacgcg ctcgcggcca cacagccaag
     1801 catcgcaagc cgggcggcga acacgctggt tcccgtaacg cggaacaacc acgcaatgag
//
LOCUS       DAOSOO010000377         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_63120_length_1850_cov_5.173259, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000377 DAOSOO010000000
VERSION     DAOSOO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63120_length_1850_cov_5.173259"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLT86_15110"
     CDS             <1..672
                     /locus_tag="PLT86_15110"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOT37991.1"
                     /translation="ACTGPGTPPDSSATTGIVLQKHVRSLGRFSPSFFQTVDERDSVF
                     SATAKSRAVRGVHGEVLARVSPRFYRRLCVEGSGRLLDGRVVTFSRRHGGEPRFRVTS
                     SQYGLTCRATPLIPYRSVAVDRRQVRLGSVLYIPAVEGLVLPDGSVHDGVFFADDVGG
                     AVRGRKMDFFVGLSSHTENPFTTSQRILHARPVEVFLIPPELAERYSRLHRVLADRES
                     ASAPD"
     gene            1019..1633
                     /locus_tag="PLT86_15115"
     CDS             1019..1633
                     /locus_tag="PLT86_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37992.1"
                     /translation="MTAASHARNTTIQRILGIARRCCVVGVLGSMCGCNPFQTGEPES
                     PTGALIQYPSPSTTDNVLAILSLSMRAKDSRAFLDRLTADFRFLPDPVQIQGPDFRTF
                     PAEWGTVQEEMALAVLFSNSDSVTVDWSSVFTQHRVEGASVGATYRVNVFSRTSLTAS
                     YTGRVEIVMTQEAGSWAVREWRDYVVSGESNTWGLLRARLLGSG"
     gene            1655..>1850
                     /locus_tag="PLT86_15120"
     CDS             1655..>1850
                     /locus_tag="PLT86_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37993.1"
                     /translation="MSRGILFVSALFVSLLAGCDLLNQTAVIDDTIATGGVSRPVTLS
                     RPDSVLKMLTYIFDRHTPESV"
BASE COUNT          350 a          566 c          534 g          400 t
ORIGIN      
        1 gcatgcaccg gccccggaac tcctccggac agttctgcaa ccaccgggat tgttcttcag
       61 aaacacgtgc gctccctggg gcgcttcagc ccatcctttt tccagaccgt agacgaacgc
      121 gacagcgtgt tttcggcaac cgcgaaatct agagcggtca ggggagttca cggtgaagtg
      181 cttgcacggg tttcaccgag gttctaccgc cggctctgcg ttgagggaag cggcagattg
      241 ctggatggcc gcgttgttac tttttcccgt cggcatggcg gagaaccgcg tttccgggtc
      301 accagttcgc agtacggcct gacgtgccgg gccactccgt tgattccgta ccgatccgtc
      361 gccgtggacc ggagacaggt caggcttggg tccgtccttt acatacccgc cgttgaggga
      421 cttgttctgc ccgatgggtc tgttcacgac ggagtgttct tcgcagatga cgttggtggc
      481 gcggtacgcg gccgaaaaat ggacttcttc gtcgggctta gcagccatac ggaaaacccg
      541 ttcaccactt cgcaacgcat tcttcacgct cgcccggtcg aagtttttct gattcctcct
      601 gaactggccg aacgctactc ccgactgcac cgcgttctgg ccgatagaga gtccgcctca
      661 gccccggatt gaggaaacgc cgttccccct tcggcgaaac aatagccgta acgctcacat
      721 tccgatcagc ccccgacaat cctcgcctga acgtggggag gccgacgtcg gaataatgct
      781 tttcccaaga atccaccgat ttcttcgcga ccgggaaacg cggtttggtg cccggacacc
      841 ctgcggcggt gggttgaaga ggtattcccc ggcataaccg atgggttcgg gacgctgcga
      901 gaggggttta tgactcccgt cagacgaact atcttccgta gaagaagcct caaacctgac
      961 gcgccgcaga gtgggcccgg gatttcggtt tcccacgttc tgaaagtggt cgccggcaat
     1021 gacagcagca tcccacgcgc gaaacaccac aatccagcga attctcggca ttgcccggcg
     1081 ttgttgcgtc gtcggtgtgc tgggatcgat gtgcggatgc aacccgttcc aaacgggcga
     1141 accggaatcc ccgaccggtg ctctgattca gtacccctca ccctcaacca ctgacaacgt
     1201 cctagcaatc ctgtcacttt ccatgcgcgc aaaagacagt cgcgcattcc tcgaccggtt
     1261 gaccgcggat ttccgtttcc tgccagatcc ggttcagata cagggtcccg atttcagaac
     1321 gtttcccgct gaatggggca ccgtccagga ggaaatggcg ttggcggtgc ttttcagtaa
     1381 cagcgacagc gtcaccgtcg attggagttc cgtcttcaca cagcatcgtg tggaaggggc
     1441 ctcagtgggc gcgacgtacc gggtcaatgt gttctcccgc acgtctctca ccgcatccta
     1501 caccggtcga gtcgagatcg tgatgacgca ggaagcaggt tcgtgggccg tgcgcgaatg
     1561 gcgagattac gtcgtgtccg gtgagtccaa tacctggggg ctgttacgag cccgattgct
     1621 cggttcggga tgaagttggg ctgaggagca accggtgtcc agaggaatcc tctttgtgag
     1681 cgcgctcttc gtgagcctcc tcgccgggtg cgaccttctg aaccagacgg ccgtcattga
     1741 tgataccatc gccaccggcg gagtcagtcg acctgtcacg ctgtcccgcc cggacagcgt
     1801 cctcaaaatg ctgacgtaca tcttcgatcg tcacaccccg gaatctgttc
//
LOCUS       DAOSOO010000378         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_65260_length_1806_cov_5.884637, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000378 DAOSOO010000000
VERSION     DAOSOO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65260_length_1806_cov_5.884637"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..915
                     /gene="carB"
                     /locus_tag="PLT86_15125"
                     /pseudo
     CDS             1..915
                     /gene="carB"
                     /locus_tag="PLT86_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878769.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
     gene            1105..>1806
                     /locus_tag="PLT86_15130"
     CDS             1105..>1806
                     /locus_tag="PLT86_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37994.1"
                     /translation="MTLRERAMTVLRGGIPDAVPWFTDLTYYQHGTTQAGTLEAKYRG
                     SEGLLQLHRDLRVGVYLFTPPLVTIERDPTLFSIEEKDIGDGKTLRVFRSPEGSLTSV
                     TQRCEDSFSSAILEYPVKTADDLRVVVAWYQGASYSPNYDAVVACDAHWGDDGLGVPL
                     TPRTPLAALVAEWCGVNNLAYLVADAPAEVEATLDAMRRAEDELYRLTCAAPYPLVEI
                     PDNLSAEAVGGLWRCF"
BASE COUNT          388 a          513 c          551 g          354 t
ORIGIN      
        1 ggaggaactg ggggagggat cgcgtacaac acggaggaat tcggcgacgt cgtttcaaac
       61 ggtctcgagc tcagtcccat tcacgaggtc cttatcgagg aatcgatcat cggctggaaa
      121 gagtacgaac tggaggtgat gcgagacctt gcggacaacg tcgtcatcat ctgctcgata
      181 gaaaacctgg acccgatggg tattcatact ggcgattcca tcactgtggc acccgcgcag
      241 acactgaccg acaaagagta ccagtgcatg agagacgccg caatcgccgt catccgtgaa
      301 atcggcgttg aaaccggcgg gtcgaatgtc cagttcgccg tcgaccccgc aaccggacgc
      361 atggtgatta tcgagatgaa tcctcgcgtc agcaggtctt ctgctcttgc gtcgaaggcc
      421 accggattcc ccatcgccaa aatcgccgcg atgctggcag tgggatatac actggacgaa
      481 atcccgaacg acatcaccaa ggttactccc gcttcgttcg aacccaccat cgattactgc
      541 gtcgtcaaga ttccccggtg ggcattcgag aaattccccg gtgcggaacc ccggttggga
      601 atccagatga aatcggtcgg cgagacaatg gccatcggca gaacattcaa ggaagcgctg
      661 cagaagggcc tgagatcact cgaaatcgac cgacacgggc tgggatcgga cgggaaagac
      721 gccggggtac cggcggacga tgccgtggcg tttttgaaag caaaccttcc cgtccccacg
      781 ccagcaaggt tgttccagat tcaggctgcc ttcgaagccg gtttcacagt ggaagatgtg
      841 gcggagctga caaagatcga cccctggttc ctgcgcaata tgaaggagat cgtggacttc
      901 tcggcccagt tgcgcacctt cgcgacgtga cagatggctt cgcgcgacgt catagccaca
      961 tcctggaccg accgggttcc catcggttag caacctgaag cgaaggcatg cggctttcgg
     1021 aatgaacgaa gggcgcgttt cccgcccctt tttgatctcg tgcgctcagt accgcctcaa
     1081 tctgtatgcc tggagggaat gaggatgacg cttcgcgagc gagcaatgac agtgttgcgc
     1141 ggcggtattc cggatgcggt gccgtggttc accgatctta cgtactacca gcacgggacc
     1201 acgcaagcgg gaacattgga ggcgaagtac cggggcagtg aagggttact gcaacttcat
     1261 cgcgacctcc gagtaggtgt ctacctcttc acaccgccct tggtcaccat cgagcgagac
     1321 ccgacacttt tttcgatcga agagaaggat atcggcgacg gcaaaaccct tcgggttttt
     1381 cgttctccgg aaggatcgct tacgagcgta acacagcgtt gcgaggattc cttttcgtcg
     1441 gcaatcctcg agtatccggt gaaaacagcc gacgatctgc gggtagttgt tgcgtggtat
     1501 cagggtgctt cctactcgcc gaactacgat gccgttgtgg catgcgacgc tcactgggga
     1561 gacgacgggc tcggggtacc cttgacgccc cgcacgccgc tggccgcgct ggtggcggaa
     1621 tggtgcgggg tgaacaatct ggcgtatctt gttgccgacg caccggcgga ggtggaggca
     1681 accctcgacg ccatgcgcag ggcggaagac gagctgtacc gcctgacatg cgcggcgccg
     1741 tacccgctgg tggagatccc cgacaacctc tctgcagagg cggtgggcgg cctctggaga
     1801 tgcttc
//
LOCUS       DAOSOO010000379         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_65625_length_1799_cov_4.010321, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000379 DAOSOO010000000
VERSION     DAOSOO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65625_length_1799_cov_4.010321"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PLT86_15135"
     CDS             complement(<1..1201)
                     /locus_tag="PLT86_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT37995.1"
                     /translation="MLRSRVVLFLLLALATSGLLESARATDVSGTITTTKWTKANSPY
                     HATGTVTVPAGNTLTIEPGVDVLFDSTAQFVVNGTLNAVGTEADSIRFQAGQAPVWGG
                     VRITGGTGSTVSYTRISGVGGVTDGGALCVTGSGTQLSVSHVVVSANQSVGVYLSDGN
                     LILTDCTISDNTQVGVYNEGTLTMVQCAISRNTGEGLANGPLDRSYDRAASVRYCVIS
                     ENSTGVWNKKGYVTIENCTISGNGTGLSNASTLTVTKTLIANNRSYGVFHYAKYWALS
                     LTDCTISGNSGVAVFNYGETVTLTRCLVVGNTSSSSMIENYAAIDRGASLIIRNCTIT
                     GNSSSGQSLIKSSRFVGQPKGSGYSNLTVQNTIVWGNIAAKQIDNVNGQATVTYSDIQ
                     QESGVFAG"
BASE COUNT          350 a          529 c          547 g          373 t
ORIGIN      
        1 tgccggcgaa gacgccggat tcctgttgga tgtcagaata ggtcaccgtt gcttgcccgt
       61 tcacgttgtc aatctgtttt gccgcgatgt tcccccacac aatggtgttc tgcaccgtga
      121 gattcgaata tcctgatccc ttgggttggc ccacaaacct gctgctcttt atcaggctct
      181 gcccgctgct ggaattgcct gtgatcgtgc agttgcgaat gatcaaactc gcaccgcggt
      241 ctatggcggc gtagttctcg atcatcgaag aagaggaggt gttgccaacc accagacacc
      301 tggttaacgt caccgtctcc ccgtaattga acacagcgac gcccgagttc cctgaaattg
      361 tgcaatccgt gagcgacaag gcccaatact tggcgtagtg aaacacaccg taactgcggt
      421 tgttggcgat gagagttttc gttacggtga gggtggatgc gttgctcagc ccggttccgt
      481 ttcctgaaat cgtgcagttc tcgattgtca cgtagccctt cttattccag acacccgtcg
      541 agttttcgga aatgacgcaa tagcgcaccg atgccgcccg gtcgtaactc cgatcaagag
      601 gcccgttggc gagcccttcc ccggtattac gagagatcgc gcactggacc atcgtcaggg
      661 taccttcgtt gtacactccg acttgcgtgt tgtctgagat cgtgcaatca gtgagaatca
      721 agttgccatc agacaggtac acgccgacag actgattggc ggacacaacc acatgggaga
      781 cagacaactg ggtcccgctt cccgtaacgc atagcgcacc tccatccgtt actccgccaa
      841 ccccgctgat gcgggtgtag gaaaccgtgc tgccggtgcc gccggtgatc ctgactccac
      901 cccaaaccgg ggcctgacct gcctggaacc tgatactgtc tgcctccgtg ccgacggcgt
      961 tgagcgttcc gttgacaaca aactgcgcgg tggagtcaaa cagcacgtcc acaccgggtt
     1021 cgatggtgag ggtgttgccg gcgggaacgg taaccgtccc cgtcgcgtga tacgggctgt
     1081 tggccttcgt ccacttcgtc gtcgtgatgg tgccggacac atcggtggcc cgggcggact
     1141 cgaggagccc gcttgtggcg agggcgagca ggaggaaaag aacaacgcga gaccgcagca
     1201 tggcgcacct ccccttgcag gcatcaaaag gcctgcccgt aaggccgatg ccttgagcgc
     1261 acatcacaat gcactgaatt tcgctgtatc aacaccggtt gtcaacgatg tgcggcgtgt
     1321 tgatcgctgg ggaatgtagt ttatccgggg ggggcaagtg gcgagggcgg ggaacagctt
     1381 cgcagggcga ctcgcactgg gatggattcc cgcctgcgcg ggaatgacgg attgtgggcg
     1441 tgttcctgat cctttggcag agaagcagtc aatgactttg gggtcgtcat ttccgacagc
     1501 gcgggggtat gccggtcctc ccgaagaagc gaacgtcatt cccgcggagg cgggaatcca
     1561 gaccgggtgg cctggtgcgg cttccgcggt catctcgata caccgtccgc cggacggtcg
     1621 gcggacgcca ctcgatgacc gctgttcggg cgccacatgc cggtcgtcga gtcgagcgca
     1681 cgcgagggag acgtatcgag acgcacgggc gcggtacatt cgagccaacg gaaggtgaaa
     1741 catgaccgcc gggccgggct cctcgatacg ccgctcggac ttcgtcctcc cgccactcg
//
LOCUS       DAOSOO010000380         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_66082_length_1790_cov_6.885303, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000380 DAOSOO010000000
VERSION     DAOSOO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66082_length_1790_cov_6.885303"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1046)
                     /locus_tag="PLT86_15140"
     CDS             complement(240..1046)
                     /locus_tag="PLT86_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT37996.1"
                     /translation="MADLARVHIGCCGWHYDDWRGPFYENDTDPDQELGSYAITFDTV
                     EIDSTFYGIPLATTVHQWYQRTPARFRFAAKVPRSVTHEGPLAEGTREFRAFCAVMRG
                     LGQKLGVLLIQLPPSFTAQSAGMLDDFLDQLPTDIRFAVEARHRSLFTDGVYDMLRHH
                     RVAWAISDITGLPIAEEVTADFSYVRWMGNRDDVRVFDRETINRSTELVAWRERIIAL
                     REQVSEIFGYFNNHYSGHAPATAQRFKEMIGLPTTRRTLWRQASLLDGLA"
BASE COUNT          319 a          555 c          601 g          315 t
ORIGIN      
        1 cacgcccaca atccgtcatt cccgcggagg cgggaatcca gaccgggtgg ccgggggcgt
       61 tattcgtggt catctcgata caccgtccgc cgaccgtccg gcggacacca ctcgatgacc
      121 gctgctcggg cgcgccgcat gccggtcgtc gagtggctca cgagcgcatg cgagggagac
      181 gtatcgagac gcaccggcac gaagacgacg ttttgcctca gtcggttcat gtccgcgatt
      241 caggccagcc cgtcgaggag actcgcttgc cgccacaacg tccgccgggt tgtgggcagc
      301 ccgatcatct ctttgaaccg ttgcgccgtt gccggtgcat gcccgctgta gtggttgttg
      361 aaatagccga aaatctcgga cacctgctcg cgcagcgcga tgattctctc ccgccatgca
      421 accagttctg tagaacggtt gatcgtttcg cggtcgaaca cgcgcacgtc atcccggttg
      481 cccatccacc ggacatagga gaaatccgcg gtcacttctt ccgcgattgg taaccccgtg
      541 atgtcggaga tggcccacgc cacacgatga tggcggagca tgtcgtacac gccgtcggtg
      601 aacagcgagc ggtggcgcgc ctcaacggca aagcggatgt cggtgggaag ctggtcgaga
      661 aagtcgtcga gcattccggc ggactgggcg gtgaagctgg ggggaagctg gatgagaagc
      721 acgccgagtt tctggccgag gccgcgcatc accgcgcaga aggcccggaa ttcccgggtc
      781 ccttccgcca gcggcccttc atgggtaacg ctgcgcggca ctttggcggc gaagcggaac
      841 cgcgcgggcg tgcgttggta ccactgatgc accgtggtgg cgagcggtat accgtagaag
      901 gtgctgtcga tctccacggt atcgaacgtg atggcgtacg agccaagttc ctggtccggg
      961 tccgtgtcgt tctcgtagaa cgggccacgc cagtcgtcgt agtgccaccc gcagcaaccg
     1021 atatgaacgc gggccaggtc tgccatgtgt cgcctcgctt gagacgggaa ccgagtctcc
     1081 tgcgctggtg cgaaaacgta ccgggggtac gcgatgtcgt caatagccgt ggtaatgccg
     1141 gcgagaaatt ccagcattga cgcggagtgg gcgtcattct gagcgcagtg aggaatctcc
     1201 gcgtggtccg cctgtcaggc atgttctgga ttcccgcctg cgcgggaatg acgatacccg
     1261 cctccggatc gtgttcatct cagcatgtgg gacccacggg aactcagcac ccactggcgc
     1321 tcgcgacgga cgctccgacg cgttgttttc cgaagggggc gtaagtccgt tcctaacgaa
     1381 tgccgtcatt cccgcgaagg cgggaatcca ggcagggtac cgcggagggg cttgtcacgc
     1441 ggcaatgcgg agcgcgtgtc ccgctcgcac tcgcgagacc caggaacagc gctcccgcgc
     1501 ggtgaggcag gataaaaccg gacccgacgg agcgggtccc tccagtgcaa cgtccccggc
     1561 ggaggggcag gcgcaagggt tggagggtca cgctccgtcg tgaccaattg ccttgctgcc
     1621 gcctgtctgt gcggcgtatc aatgcatcgt gccggcactt ccacaggctt ccgcgcgttg
     1681 ggcgcacgct gagggactgc tcagggatgg gcgctcgcac cggcggaacc ccgggacagc
     1741 gctcccgcgc ggtgaggcag gataaaaccg gacccgacgg agcgggtccc
//
LOCUS       DAOSOO010000381         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_66453_length_1783_cov_5.693866, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000381 DAOSOO010000000
VERSION     DAOSOO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66453_length_1783_cov_5.693866"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1783
                     /locus_tag="PLT86_15145"
     CDS             36..>1783
                     /locus_tag="PLT86_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37997.1"
                     /translation="MQYVPWGWEPAAVRVAAQLGGEPNLLAWSIGDDVPLGGMEPLSA
                     VARQLREAAPGIPTTADCIPSLGRLDSVRRTFAEFVDITMNYDYPIPDAEYPLGGNAF
                     RRHQAFFDEQRAMFGDPRWTWTQTYLWHWTGQDLNVSPEAAGPYPEPEQVRLLVFSEI
                     NAGLRGLFCFSHREIALQPNLAAEIAVTFREIALFNDFLAAGEIRRSLTTSDTTLDAT
                     AFSYRGSTLVSLALLRERYQYWIGEGIARDVSVDVPWPGARLPNALLVATPDVIECRA
                     VRVDPQTVRITIPSLELAGFVFLSGDSRELARVRSRVAAIPREVRTLLVPGAMAQVQK
                     VNQVVWQSGACDMQNTVISWAPVQAVERCSRAIDERRYADAVTEWKTANRLCRVALDS
                     VMRYAGARADQLTADERRHLLTPYGLHNIPALAFASPPGDRWRFVREWTVVGPFPLNR
                     DWNDFDAVPEGFHTAYPPETSADRHGVFDCIGGPAEWRYARSDLSGRLDFLQYFSPTE
                     NVLAYARCRVIVPRDTTVNFSIGSNDGIKVWANGELAFELPVPRGRKAVRHQNQFPVH
                     LRAGENRILVKLTNLGS"
BASE COUNT          300 a          549 c          616 g          318 t
ORIGIN      
        1 acatgcggga gtgcaagcgg cacggcgtgc aggtgatgca gtacgttccg tggggatggg
       61 aaccggcggc ggtccgggtt gccgcgcagc tcggcgggga accgaacctg ctggcatgga
      121 gcatcggtga tgacgttccg ctgggtggga tggagccgct gagcgcagtt gcccggcaac
      181 tgcgcgaggc tgcacctggg attccgacca cggcggactg cattccttcg ctcggacgtc
      241 tcgactctgt caggcgcacg ttcgccgagt tcgttgacat cacgatgaac tacgactacc
      301 cgattcccga cgcggaatac ccgcttggcg gcaacgcatt ccggcggcac caggcgttct
      361 tcgacgagca gcgggcgatg ttcggcgacc cacggtggac gtggacgcag acctacctct
      421 ggcactggac ggggcaggac ctgaatgtga gcccggaagc cgcagggccc tacccggagc
      481 cggagcaggt gcgcctgctt gtcttttcgg aaatcaacgc cggtctgcgc gggttgttct
      541 gtttttcgca ccgggagatt gccctccagc cgaaccttgc ggcagagatc gccgtaacgt
      601 tccgggagat tgcgctcttc aacgatttcc ttgcggcagg ggaaatccgg cgttctctca
      661 ccacatccga cacgacgctc gacgcaacgg cgttttcgta ccgagggtca acgctggtct
      721 cgctggcgct cctgcgggaa cggtatcagt actggatagg cgagggaatc gcacgggacg
      781 tgtcggtgga cgtgccgtgg cccggtgccc gtctgccgaa cgcactgctt gtggcgacgc
      841 ctgatgtcat cgagtgccgc gcagtcaggg tggatccgca aacagttcgc atcacgattc
      901 cctcgctgga gctggccggg ttcgtgttcc tgtccggcga ttcgcgcgaa ctggcccggg
      961 tgcggagccg cgtggccgca atcccccgcg aggtgcgcac gcttctggtt cccggtgcga
     1021 tggcgcaggt gcagaaggtg aaccaggtcg tgtggcaatc tggcgcgtgc gacatgcaga
     1081 acacggtcat cagctgggct ccggtgcaag cagtggagcg gtgcagccgg gcgattgacg
     1141 agcggcgcta cgcggacgcc gtgaccgaat ggaaaacggc gaaccgtctg tgccgcgtgg
     1201 cgctggacag tgtcatgcgg tacgccggcg cgcgggcgga tcaactcacg gccgacgagc
     1261 ggcggcacct gctcacgccg tatggcctgc acaacatccc ggcattggcg ttcgcttccc
     1321 cgccgggcga ccggtggcgg tttgtgcgcg aatggacggt ggtcgggccg tttccgctca
     1381 accgcgactg gaacgatttc gatgcagtgc cggaagggtt ccacacggcc tatccacctg
     1441 aaaccagcgc cgaccgtcac ggtgtgttcg attgcatcgg cggccctgcg gagtggcgtt
     1501 atgcgaggag cgatctgtcc ggaaggctgg atttcctgca gtacttcagc ccgacggaga
     1561 acgttctcgc gtacgcccgg tgccgagtga tcgttccgcg cgacaccacc gtgaacttca
     1621 gcatcggcag caacgacggc atcaaggtgt gggcgaacgg ggaactcgcg ttcgagctgc
     1681 cggtcccccg cggcaggaag gcggtgcgcc atcagaacca gttcccggtg cacctgcgag
     1741 cgggcgaaaa ccgcatcctc gtcaagctga cgaatctcgg ctc
//
LOCUS       DAOSOO010000382         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_68076_length_1752_cov_5.731291, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000382 DAOSOO010000000
VERSION     DAOSOO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68076_length_1752_cov_5.731291"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLT86_15150"
     CDS             complement(<1..309)
                     /locus_tag="PLT86_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37998.1"
                     /translation="MKHSVLVFVLLLVSAISVAGQAIEEDAFLLPDRVRSNPDKFTFA
                     ILGDRTGSDRQSWRIFDRSVEEINLSRPDFVIMVGDMIEGHDYPEEVAAQWEEFQSHA
                     D"
     gene            639..>1752
                     /locus_tag="PLT86_15155"
     CDS             639..>1752
                     /locus_tag="PLT86_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT37999.1"
                     /translation="MPRPATVLGARIGLESNEAAFFARADTTLWVSARRTLSRLAGSG
                     VSQFDWVTYDVGTITSRPDEAITAYAVSGDRIAAATSHVVNSSSGSYYVGDGLYLSRD
                     GGASWFPIPMTALFADRTNMAVPGGDVQSFGVWFDGTDLWAAFTTEFAVMTPDFGVTW
                     HRYRPDSTNNPQPNPFTGDPGRLHRYRHLNYRAFDGLAVHDTLWVSTNAGINRSTDRG
                     VMWQNFDAARAGISGDFVPSVAVDTVNGILWASTQSTGLDENYLKNNPQDVFTDGVFD
                     SLDYDLDRDGILDGPGKNGVSWTRDGGQTWHTYVPVDDATVGVDYRAWGFAFNGQKVL
                     VGGSIGSFDALLRSDDLGATWRLQPIRTALGDTSRSG"
BASE COUNT          316 a          495 c          568 g          373 t
ORIGIN      
        1 gtccgcatgg ctctggaatt cctcccactg cgcggcgacc tcttccgggt agtcgtgccc
       61 ctcaatcatg tcgccgacca taatgacgaa atcggggcgg gagaggttga tctcctctac
      121 cgagcggtcg aaaatgcgcc aactctggcg atcggaaccg gttctgtcgc ccaggatggc
      181 gaaggtgaat ttgtccggat tgctgcgcac gcggtccggt aacaggaagg cgtcctcctc
      241 gatggcctgg ccggcaaccg aaatggcgga aacgaggagc agcacaaaaa caagaaccga
      301 atgtttcatg aatcattccc gaaaaatcta cccgattgtc gaggccctcg ccgggacgcg
      361 ttttctgtcc ggcggatggt ctattcgtcg gggctggtct tgaataaata aagcgcgttc
      421 gccggtctta tggttccctg ccccgcgggc gcatttgtct cgagcggcgc cggttgttgt
      481 tgtttgagag cggcgagggg tatttttagc atgtttccgc aggaacaacc ggtgtcccct
      541 tccgaggaag gtatccgctg aacacgagac agtttctccg cgtcattgcc tttgcgggcg
      601 ttgcaggcct gttcccgaac accgttgtgc acgcgcagat gcctcgcccg gcgacggtgc
      661 tgggtgcgcg aatcgggctg gaaagtaacg aagcagcctt tttcgcacgc gccgacacca
      721 cgctgtgggt gagcgcgcgc cgcacgctga gccgccttgc cggttccggt gtatcgcagt
      781 tcgactgggt gacgtacgac gtaggaacga ttacgtcgcg cccggatgag gcgatcacgg
      841 catacgcggt gagtggggat cgcattgcgg cggccacttc gcacgtcgtg aacagctcgt
      901 ccggcagcta ctacgtgggc gacggtctgt atctttcccg cgacggcggt gcgagctggt
      961 ttccgattcc catgacggcc ctttttgccg atcgaacgaa catggcggtt ccgggcggcg
     1021 atgttcagtc attcggcgtg tggtttgacg gcacggacct ctgggcggca tttaccaccg
     1081 agtttgccgt catgacgccg gatttcgggg tcacgtggca ccggtaccgg cccgattcca
     1141 ccaacaaccc ccagccgaat ccatttaccg gggatcccgg gcggctgcac cggtaccggc
     1201 atctgaatta ccgcgcgttt gatggcctcg cggtgcatga cacgctctgg gtttccacca
     1261 acgcgggcat caaccggtct acggatcgcg gcgtcatgtg gcagaacttt gacgcagcca
     1321 gggcaggcat cagcggggat tttgttccct cggtcgcggt ggatacggtc aatggaatcc
     1381 tctgggcgtc tactcagtct acgggactgg atgagaacta tctgaaaaac aaccctcagg
     1441 atgtgttcac ggacggtgtg tttgattcgc ttgattacga tcttgaccgc gatggaatac
     1501 tcgacgggcc cggaaagaac ggggtaagct ggacgaggga tgggggtcag acctggcaca
     1561 cgtacgtgcc ggttgacgat gccaccgtcg gggtggatta ccgggcgtgg ggttttgcgt
     1621 tcaacgggca aaaagtgctt gtcggcggct caatcgggtc gtttgacgcg ttgctgcgga
     1681 gcgacgatct gggcgcgacg tggcgtcttc agccgatccg aacggcgctc ggagatacgt
     1741 cccgttcggg gc
//
LOCUS       DAOSOO010000383         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_68139_length_1751_cov_5.814269, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000383 DAOSOO010000000
VERSION     DAOSOO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68139_length_1751_cov_5.814269"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1718
                     /locus_tag="PLT86_15160"
     CDS             309..1718
                     /locus_tag="PLT86_15160"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOT38000.1"
                     /translation="MTTPPPVAKTLEEANAIIAKLWARVQELEARVEELTARLAQNST
                     NSSRPPSSDPLWSKPKRKPPVAGSGRKPGGQPGHAAHVREPVPPEQVDETVDVYPDRC
                     GICGAKLEAEFPADDAYAHQVTDLPPARPHVTEYRLWRTRCPHCGGRTRAKRPANVPE
                     DAFGPRLKAVVAALTSKYRVSRREMVEVLHDLCGVEMSVGAVQATCERVSVAVSPAVE
                     AVRTEVVSAPVVHADETGWRQKREMHWLWGATTPNAAYFVLAGDRGRAALSQLLPDTY
                     PGVVHCDRWRPYQRFGDNRQLCHAHLRRDFQAAIDRGGKARPIGRNLLASSDRLFSVW
                     HAFRRGEINRQQMARDMEPIQAEWEQSIASAIESEDSKLSALGADLFKQWDVLWTFVR
                     VEGVEPTNNDAERALRPAVLWRKGSFGTRSDKGSAFVAKLLTIVHTAKRRKVDVVTWL
                     RDACDNAAAGLAPAPLIVG"
BASE COUNT          331 a          547 c          587 g          286 t
ORIGIN      
        1 tgaaacaatc atctttggat gaatttgcag gtatcctgtc tgccggattg ccagaactcc
       61 cagatgaaaa accatccttc aaggatacat cgcgactttt ctcgtaatcc cggacgaact
      121 caatatagta attgaacttg tcgatccaaa gttccccgtt gatctcacat gcatcgaacc
      181 gtgggtggtt tttatcgatt ccaggaatcg cgattctcca aacaggtatt ggtaaccgtt
      241 cacccccctc gacgcagtgc tggctgaaaa cttgacggca ggcggaagca tgcgactctt
      301 ccccggtgat gacaacgcca ccaccggtag cgaagacgct ggaagaggcc aacgcgatca
      361 tcgcgaagtt gtgggctcgg gtgcaggaac tggaagcacg ggtcgaggaa ctgacggccc
      421 gtcttgcgca gaactcgacg aactcgtcgc gaccgccatc gtcggatccg ctgtggtcga
      481 agccgaagcg gaagccaccg gttgcaggaa gcggtcggaa gccgggcggg cagccagggc
      541 atgcggcgca tgttcgcgag ccggttccgc cggaacaggt ggacgagacg gtcgacgtgt
      601 acccggaccg atgcggcatc tgcggggcga agttggaagc ggagtttccg gcagacgacg
      661 cgtacgcgca ccaggtgacg gacctgccgc cagcgcgccc gcatgtgacg gagtaccgtc
      721 tgtggcggac gcgttgcccg cactgcggag gccggacgcg tgcgaagcgt cccgcgaacg
      781 tgcccgagga cgcattcggc ccccggttga aggcggtggt cgcggcgttg acgtcgaagt
      841 accgggtgag ccggcgggag atggtcgagg tgctgcacga cctctgcggc gtcgagatgt
      901 cggtcggcgc ggtgcaggcg acgtgcgagc gggtcagcgt cgcggtgtcg cctgcggtcg
      961 aggcggtgcg gaccgaggtc gtgtcggccc cggtcgtcca cgcggacgag acgggctggc
     1021 ggcagaagcg cgagatgcac tggctgtggg gcgcgacgac gccgaacgcc gcgtatttcg
     1081 tcctggccgg agaccgaggc cgcgcggcgc tgtcgcagtt gctgcccgac acgtatcccg
     1141 gcgtcgtgca ttgcgatcgc tggcgaccgt accagcgctt tggagacaac cgccaactct
     1201 gccacgccca cctgcggcgg gacttccagg ccgccatcga ccggggcggc aaggccaggc
     1261 cgatcggcag gaacctgctc gcctcgagcg accgcctctt ctctgtgtgg cacgcgttcc
     1321 gacgcggcga aatcaaccgg caacagatgg cgcgggacat ggaaccgatc caggcggaat
     1381 gggaacagtc catcgcctct gcgatcgaga gcgaggactc gaaactctcg gccctgggcg
     1441 cggacctctt caagcaatgg gacgtcctgt ggaccttcgt ccgggtcgag ggagtggagc
     1501 cgaccaacaa cgacgccgag cgtgccctcc gacccgccgt cctgtggagg aagggctcct
     1561 tcggaacccg cagcgacaag ggcagcgcct tcgttgcaaa gctgctcacc atcgtccaca
     1621 ccgccaagcg caggaaggtc gatgtcgtca cctggctccg cgacgcctgc gacaacgccg
     1681 ccgccggcct cgctccggca cccttgatcg tgggatgagc ccgtgtcaca ctacacccag
     1741 tgaatggtta c
//
LOCUS       DAOSOO010000384         1722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_69831_length_1722_cov_5.879424, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000384 DAOSOO010000000
VERSION     DAOSOO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69831_length_1722_cov_5.879424"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLT86_15165"
     CDS             complement(<1..1642)
                     /locus_tag="PLT86_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38001.1"
                     /translation="MRHPSHYRVIWHEVGHALQYMDFPIVPENVLKKAVLPITRTQVD
                     CLSWLAHTYLGGPYDMPRMWRDYLDPSRYEYVDPEGRPLGMFMEHARPDPDHYRDEGQ
                     RRRYGTAHELWKQGADVVDLVAAASRRYGLDFTLAFRMNDYHHGSWEEHPRWWLEHRG
                     MTVGKGRQPDDNSPAAARRISGLDFTHPEVREHVIAPIIAAAECYDVDGVDLDFSRSR
                     PYFPPGEEKPECLTELVKTVRERLEPIFRKRGRKPRVLARIYERPTCDDAGMDWLTWM
                     EEGLIDALSIGRMYGADYRDVVKVARQHNVEVYAGFDCIAFNLNDTKHFGRLEYFRAA
                     CLNYYHQGVNGVFFFNGSALWSGPYPGPVARAMPHLMEVGDPGCLERRSKIYVTGWHV
                     DHKPQLIWSSDPERDPGGAARSFEVSDNLRDSFDCGDLERLTLKVEFKLTTPWISELN
                     EWHLIWSRLDTMEYLLNGQPVEPDPIEKDELTPYALDGADINKNWSRVIRFDLTHGPL
                     PVCGQNSFFVRVLEPESQLVGPRDLLLGRLELYVDYGEP"
BASE COUNT          333 a          513 c          518 g          358 t
ORIGIN      
        1 ccggttcccc gtaatccacg tacagttcga gacgacccag gaggagatcg cgcgggccga
       61 caagctggct ttccggctcg aggactcgca cgaagaagct gttctgcccg cacacgggca
      121 acgggccgtg cgtgaggtcg aagcggataa cccggctcca gttcttgttg atgtcggcgc
      181 cgtccaatgc gtacggggtc aactcgtctt tttcgatggg gtctggctcg accggctggc
      241 cgttgagcag gtattccatc gtatcaagac ggctccagat gaggtgccac tcgttcaatt
      301 cgcttatcca tggcgtggtg agtttgaact ccaccttcaa cgtgaggcgc tcgaggtcgc
      361 cgcaatcgaa ggaatcacgc aggttgtcgc tcacctcgaa cgatcgcgcc gcaccgccgg
      421 gatcccgttc cgggtcggat gaccagataa gctgaggttt gtggtccacg tgccagccgg
      481 tcacgtagat cttgctcctg cgctcgagac aaccgggatc tccaacttcc attagatggg
      541 gcatcgcgcg cgctacaggg ccggggtagg ggccagacca gagtgccgat ccgttgaaga
      601 agaagactcc gttcacccct tgatggtagt agttgaggca ggctgcgcgg aagtattcca
      661 atcgtccaaa atgctttgtg tcgttcagat tgaacgcgat gcagtcgaat cccgcgtaca
      721 cctccacgtt gtgctgccgg gcaaccttca ctacatcacg gtaatctgcg ccgtacatcc
      781 tgccgatgga aagcgcgtca ataaggccct cctccatcca cgtgagccaa tccatgccgg
      841 cgtcgtcgca ggtcgggcgc tcatagatgc gcgccaagac gcgaggtttg cggcctcgtt
      901 ttcggaaaat gggctccaag cgttcgcgca ccgtcttgac aagttccgta aggcactccg
      961 gcttctcctc acccggaggg aaataggggc ggcttcggga gaaatcgaga tccacgccat
     1021 ccacatcgta gcactccgcg gcggcgataa ttggcgcgat gacatgttcc cgcacctccg
     1081 ggtgcgtgaa atcaagaccg gatatccttc gcgcagccgc cggggagttg tcatccggtt
     1141 gtcggccttt ccccaccgtc attccgcgat gctcaagcca ccaccgcggg tgttcctccc
     1201 atgagccatg gtggtagtca ttcattcgga aggcaagtgt gaaatcgagc ccgtaccgac
     1261 gggaggccgc cgccacgagg tccaccacat ccgcgccctg tttccagagc tcgtgtgcgg
     1321 ttccgtaccg gcgacgctgt ccttcgtcgc ggtaatggtc tggatcgggc cgggcgtgct
     1381 ccatgaacat gccgagcgga cggccctccg ggtcaacgta ttcgtaccgt gagggatcga
     1441 gataatcgcg ccacatgcgg ggcatatcgt acggcccgcc gaggtaggtg tgcgcgagcc
     1501 aggagaggca atctacctgc gtgcgtgtga ttgggagcac tgccttcttc agaacgttct
     1561 ccgggacgat cggaaagtcc atgtactgaa gcgcgtggcc gacttcgtgc caaatgacgc
     1621 ggtaatgcga tgggtgacgc atgttgttct ccagtgcatc acgaggttat ttcccgaact
     1681 agttcaggaa cagattcttc gcttcgctca gaatgacggt ca
//
LOCUS       DAOSOO010000385         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_73182_length_1664_cov_5.279677, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000385 DAOSOO010000000
VERSION     DAOSOO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73182_length_1664_cov_5.279677"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PLT86_15170"
     CDS             complement(<1..1365)
                     /locus_tag="PLT86_15170"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT38002.1"
                     /translation="MSNCKRKLHGQIFALMTFLGVSGIVSAQTTTMPNVVGMDIASAT
                     AAVKGTIARFATLYVEPTDDPAAVGKIDGQYPTEGSTLSSSTVVTLTARRANTPGLLT
                     LVSPAKGATDVSVGDTVVLTSTHPLTYYEDYWNPGNYAFSDFYLNPYRLGTSWSEDFG
                     IRWFSVSNDSRTAYGVIQWIAPDAVYVLQAEPYYNSWMYNTRTGSPTYFSSGVPIPGG
                     SVEGAAVFPAGFNGWTVDPMQSEIWLRPENWSIPTKPAEAASNQYYSDGFWPTLGTGL
                     ATLSPMSGNRSAFSIKPVAPGNNQVMGRARLHSGDPRFGRFLFGMYDANQDGVADNIS
                     VGTSRVLVDVPMTTSPFGIAQGDAKFLNTGVTRFRFDTGTEPANPDLADIVVTPIDDG
                     YSFRFIMSAGREGVLFTIVDSGYNNMTVGDRIPVQPNIPSLSEPERWVSSTEVLFGGA
                     ASSYD"
BASE COUNT          337 a          527 c          460 g          340 t
ORIGIN      
        1 gtcataggag gaggcggcac ctccgaacag cacctccgtg ctggataccc agcgctccgg
       61 ctccgaaagg gagggaatat tcggttggac cggaatgcgg tcccccaccg tcatgttgtt
      121 gtaaccggag tcaacgatgg tgaaaagcac accctcccgc cctgcggaca tgatgaaacg
      181 aaagctgtaa ccgtcgtcaa tcggagtcac cacgatgtcg gccagatctg gattggcagg
      241 ctccgttccc gtatcaaacc ggaaccgcgt gaccccggtg ttgaggaact tcgcgtctcc
      301 ctgcgcaatg ccgaacgggc tggtcgtcat cggcacgtca accagcacgc gcgaagtacc
      361 cacgctgatg ttgtcggcca ctccgtcctg attggcgtcg tacatgccaa acaggaatcg
      421 cccgaaccgc ggatcaccac tgtggagacg cgctctaccc atgacctggt tgttgcccgg
      481 cgcaaccggc ttgatagaga acgccgaccg gttgccgctc atcgggctca acgttgccag
      541 gccggtgccg agggtcggcc agaacccgtc ggagtaatac tggttcgagg cggcctccgc
      601 cggttttgtc ggtatcgacc agttctcagg ccggagccag atttccgact gcatggggtc
      661 aaccgtccag ccgttgaaac ccgccggaaa cactgccgcc ccttccaccg acccaccggg
      721 aatcggtact ccgctgctga aatacgtggg cgagccggtt cgcgtgttat acatccagct
      781 attgtagtag ggttctgcct gcagaacgta cactgcgtcc ggcgcaatcc actgaatcac
      841 cccgtacgcg gtgcgcgaat cgttcgacac gctgaaccag cgaatgccaa agtcctcgga
      901 ccaggaagtc cccaggcggt aagggttcag gtaaaaatcg ctgaaggcgt aatttcccgg
      961 attccagtag tcctcgtaat acgtgagcgg gtgggtgctc gtaagcacca ccgtgtcccc
     1021 taccgacaca tccgtggcac cctttgccgg ggatacgagc gtcagtagac cgggggtgtt
     1081 cgcccgacgc gccgtgagcg tcaccacagt cgatgaggag agtgtcgatc cctcggtcgg
     1141 gtactggccg tcaattttgc caacggcggc cgggtcatcc gtcggctcga cgtacagcgt
     1201 cgcaaaccgc gcgatggttc ccttcaccgc cgctgtggcc gaggctatat ccatacccac
     1261 cacgttcggc atcgtggtgg tctgtgcgga cacaatcccg ctgacgccca gaaacgtcat
     1321 gagcgcgaaa atctgcccgt gcaacttcct tttgcaattc gacatgcctg ttctccttca
     1381 aaccagaact accgttgttc cgatagccct tcgcactgaa ccggttcgta cccgaataaa
     1441 acgttcacgc gggaaatatt ctgcgtgtct gttcatctgt atcagagcaa caatcgcgcc
     1501 gcatttcgca ttcccgtcat attcctgctc atctaggcaa aatatacggt tttcgtgccg
     1561 caatcgtaca cgaatctctg tacgcgcgga cgccgctttg gtgcctgcta gtctttcccc
     1621 cgggtgctcc cctcctccgc gtatcggatc cgatacaaaa cggg
//
LOCUS       DAOSOO010000386         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_73437_length_1660_cov_5.336449, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000386 DAOSOO010000000
VERSION     DAOSOO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73437_length_1660_cov_5.336449"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1660)
                     /locus_tag="PLT86_15175"
     CDS             complement(<1..>1660)
                     /locus_tag="PLT86_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT38003.1"
                     /translation="KQRVMLGDEPVSLSIALHPEVLPGQTVLVTASRVKDREAPVTFT
                     NLPREEIARTYSVQDVPVLLQSLPSVHAYSDAGNGVGYSYLSVRGFGQKRVNVLINGI
                     PQNGPTSHEVYWVDLPDVLSNVQDIQVQRGVGSSLYGSSAIGGSINVITTNFNPERSV
                     TAFAGAGSYGTRKLSVAFNSGLVDDSYAMYGRFSRITSDGYRQQSWSDLWSYFLGVRR
                     FDPSMTTTLLVYGGPERTHLAYNGVTRAYLDGTVSGDARADRRYNPLTYENEIDTFNQ
                     PHYELHHDWQIAPALRLNTTLYAITGEGYYDQFRKARSLEEFRLPTFRVKDPSLYPLS
                     WYTRNADGTPRADANGDYTITKSDLTRRRNVENREYGVLPRMAWEHHGGTLTAGGELR
                     AHSGRHWGQITWLAAPPPGVAPGHLYYDYRERKLTTSAFVQEMAHILPRVTLMADLQA
                     VRHLYTLDRDSLTRYRYDAEFRWISPKAGVNVNITDRLHAFTNIAYARREPITDDLAD
                     PQDYWNEPSFAERETTFVGGQIGQIRLKNPTVKPERVLNFELGTG"
BASE COUNT          269 a          491 c          561 g          339 t
ORIGIN      
        1 cccggtgcca agttcgaaat tcagtacgcg ttccggcttg actgtggggt tcttgagccg
       61 aatctgcccg atctgcccgc cgacgaaggt ggtttcccgc tccgcgaaac tcggttcgtt
      121 ccagtaatcc tgcgggtcgg cgaggtcgtc ggtgatcggt tccctcctcg cgtaggcgat
      181 gttggtgaat gcgtgcaggc gatcggtgat gttgacgttc accccggctt tcggtgatat
      241 ccagcggaac tccgcatcgt accggtaacg ggtgaggctg tcgcgatcca gcgtgtacag
      301 gtggcgcacg gcctgcaggt cggccatgag ggtgacccgc ggcagaatgt gcgccatttc
      361 ctgcacgaac gcagaggtgg tgagcttccg ttcgcggtag tcatagtata aatgaccggg
      421 agcaacgccc ggaggcgggg ccgccagcca cgtgatctgt ccccagtggc gcccgctgtg
      481 cgcgcgcagt tctccgcccg ccgtcagagt tccgccgtgg tgttcccacg ccatgcgggg
      541 cagcacgccg tattcgcggt tttccacgtt tctgcggcgg gtgagatccg acttcgtgat
      601 ggtgtagtcg ccattcgcgt ccgcgcgggg cgttccgtcc gcgttccgtg tgtaccacga
      661 cagcggatac aacgaggggt ccttcacgcg gaatgtcggc aggcggaatt cctccagact
      721 gcgggccttg cggaactgat cgtaataccc ttccccggtg atcgcgtaca gcgtggtgtt
      781 gaggcgaagg gcgggcgcaa tctgccagtc gtggtgcagt tcgtagtgcg gctggttgaa
      841 ggtatcgatt tcgttctcgt aggtaagcgg gttataccgg cgatcggcgc gggcatcgcc
      901 cgacaccgtt ccgtcgagat aggcccgcgt tactccattg taggcgaggt gggttctttc
      961 cggcccgccg tacacgagca gggtcgtcgt catggacgga tcgaatcgcc gcacgccgag
     1021 gaagtagctc cacaggtcgg accacgattg ctgccggtat ccgtcgctgg taatgcgcga
     1081 gaaccgcccg tacatcgcgt aggagtcgtc caccagcccg ctgttgaatg ccacgctcag
     1141 tttgcgggtt ccgtaacttc ccgcccccgc aaaggcggtg acgctgcgct ccgggttgaa
     1201 attggtggtg atgacgttga tggagccgcc gattgctgat gagccgtaca gcgagctgcc
     1261 gacgccgcgc tgcacctgga tatcctgcac gttggagagc acgtcgggga gatcaaccca
     1321 gtacacctcg tgcgacgtgg ggccgttctg cggaatgccg ttgatcagca cgttcactcg
     1381 cttctggccg aacccgcgga cgctgagata gctgtaccct acgccgttgc ccgcatcgga
     1441 gtaggcgtgg acggatggga gcgactgcag caggaccggc acgtcctgca ccgagtaagt
     1501 gcgggcgatc tcctcgcggg ggaggtttgt gaacgtgacc ggggcctcgc ggtctttcac
     1561 gcgggatgcg gtgacgagca ccgtctgtcc cggaagcacc tcggggtgca gtgcgatgga
     1621 gagcgacacc ggctcatcgc cgagcatgac acgctgtttg
//
LOCUS       DAOSOO010000387         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_75713_length_1625_cov_2.029936, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000387 DAOSOO010000000
VERSION     DAOSOO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75713_length_1625_cov_2.029936"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1625
                     /locus_tag="PLT86_15180"
     CDS             <1..>1625
                     /locus_tag="PLT86_15180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT38004.1"
                     /translation="ALLNIKQEVFPDIAPDTVSISVAYPGASPEEVENGIILAIEEAV
                     RSLEGVEEVTSTAREGNGSVMVELLLGEDVNRLGQEIKSEVDRIVTFPEEAEEPEVRI
                     DAHRRDVIEVVVYGDAPDTTLHQVAEQVRDWLLQSPDITQVDLEGTPPLEIGIEISQE
                     NLRRYQTTPEEIARTLANASIDLPGGGLKTDAGEILLRLKERRDYGRQFAEVPVVTTE
                     LGSQVLLKDIAHIDDSFADTDRYSKFNGKRSVHLEVYRVGEETPMRVADAVHRLLEEY
                     QPYLPPGIEASTHHDRSETYRQRVDLLVKNGVLGLVLVLVLLGVFLEARLAFWVMMGV
                     PVSFMGSFLFLPAGDVTINMMSLFGFIIALGIVVDTNIVVGENIYYYHQEGLPFIEAA
                     IRGTREVASPVIFSVLTNIVGFVPIYFIPGYVGKTFQMIPVVVCVVFVISLIESLYVL
                     PSQLAHHQDRARKGIKRWLHGKQQAFSRRFLHWVRDYYGPALEYALRHRYITIAVGIG
                     ALALSLSYALSGRMGFQQFPIIESDYADAQVVL"
BASE COUNT          296 a          455 c          548 g          326 t
ORIGIN      
        1 tgcactgctg aacatcaagc aggaagtttt tcctgacatc gctccggaca cggtttcgat
       61 ttcggtggcg tacccgggcg ccagcccgga ggaggtggag aacgggatca tcctcgccat
      121 cgaagaggcc gtccggtctc ttgaaggcgt tgaagaagtg acctcgaccg cgcgcgaagg
      181 caacggcagc gtgatggtgg aactgctcct gggggaagac gtgaaccggc ttggccagga
      241 gatcaagagc gaggtcgacc ggatcgtgac gtttccggag gaggccgagg aaccggaggt
      301 ccggattgac gcgcaccgcc gcgacgtgat cgaggtggtg gtgtacggcg atgcgcccga
      361 caccacgctc caccaggtgg ccgaacaagt gcgcgactgg ctgcttcagt cgccggacat
      421 cacccaggtg gacctggaag gcacgccgcc cctggagatc ggcattgaga tctcgcagga
      481 gaatctccgc cggtatcaga cgacgccgga ggagatcgcg cggaccctgg cgaacgcgtc
      541 gatcgatctt cccgggggcg gcttgaaaac ggatgcgggc gagattctgc tgcgcctgaa
      601 agagcggcgc gattacgggc gccaattcgc ggaagtgccc gtggtcacca ccgaactggg
      661 cagccaggtc ctcttgaagg acattgccca tatcgacgac agttttgccg acacggaccg
      721 ctattccaag tttaacggaa agcgttcggt gcatctcgaa gtgtaccgcg tcggggagga
      781 gacgcccatg cgggtggcgg acgcggtgca ccggctgctg gaggagtacc agccgtatct
      841 tccgccgggc atcgaagcct caacccatca tgatcgctcc gaaacctacc gccagcgcgt
      901 cgatctgctg gtcaagaacg gtgtcctggg gctggtgctg gtactggtgc tgctgggcgt
      961 gttcctggaa gcgcgcctgg cgttctgggt catgatgggc gtgccggtat cgttcatggg
     1021 ctcttttctg tttcttccgg cgggcgacgt gacgatcaac atgatgtcgc tgttcggctt
     1081 catcatcgcg ctggggatcg tggtggacac caacatcgtg gtgggcgaga acatctacta
     1141 ttatcatcag gaaggcctgc cgttcatcga ggcggcgatt cgcggcacac gggaagtcgc
     1201 gtcgccggtc atcttcagcg tgctgacgaa tattgtgggg ttcgtgccca tttatttcat
     1261 tccggggtac gttggcaaga cgtttcagat gatccccgtg gtggtgtgcg tggtttttgt
     1321 gatctcgctg atcgagagcc tgtacgttct gccctcgcag ctggcgcatc atcaggaccg
     1381 ggcgcgaaag ggcatcaagc gctggctgca cgggaaacag caggcgttca gccgccggtt
     1441 tctgcactgg gtgcgagact actacggccc ggcgctggaa tatgcgctgc ggcaccggta
     1501 catcacgatc gcggtgggta ttggcgcgct tgcgctgagc ctgtcgtatg ccttgagcgg
     1561 ccggatgggc ttccagcaat ttcccatcat cgaatcggac tatgccgacg cgcaggtggt
     1621 cttgc
//
LOCUS       DAOSOO010000388         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_76647_length_1609_cov_4.148005, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000388 DAOSOO010000000
VERSION     DAOSOO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76647_length_1609_cov_4.148005"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLT86_15185"
     CDS             <1..1071
                     /locus_tag="PLT86_15185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT38005.1"
                     /translation="VLLTYGLTLVVVSGTGRFAIRGLQRRLFLRGIGLRNALVVGTGQ
                     SAVQFAETLKRTPRLGLRVAGFLATETVTNPSPLAQPILGRLNELGPVIEQHQVTDVI
                     FAEPSLSHKTVLDAVALCSGRKVNFGSLPDLYDVVGRTGAGGQLYGVPLLPLYPSDMP
                     VWQRRTKRVLDFLVAFFALVAGLPLWVLVALAVWLEDKGPVFYSQDRVGKDGRVFRFH
                     KFRSMIPNAEKATGPVWAQKNDPRITTVGSIIRKLRIDEVPQLWNVLRGEMSLVGPRP
                     ERPYFVERLAAEIPLYRRRLLVTPGITGWAQTKHAYDQTLDDVREKLKYDLYYIENMS
                     LRFDFLIILRTIWVMLTGKGAQ"
     gene            1068..>1609
                     /locus_tag="PLT86_15190"
     CDS             1068..>1609
                     /locus_tag="PLT86_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38006.1"
                     /translation="MRYSLSPFSPVRSGAHRRPGAWVRLRWLRPGLFALGAIVVAAFV
                     LYTLLVVRTLRQDAQRVAKLYAEKILPRAYSDPTITPGELRLLFDLIREMPVAVIVTD
                     DTGNPVSWKGIDVPDTARSPQAMARIRRVVEDMDRSMPPRKVLVPLPDNRSLQMEIHV
                     AESAFLRRIAWMPVVAAIVTL"
BASE COUNT          306 a          484 c          485 g          334 t
ORIGIN      
        1 gtgctgctca cgtacggact gacgctcgtc gttgtcagtg gcacagggcg atttgccata
       61 cgcgggctgc aacgacgcct gttcctgcgc ggaatcggtc tcagaaacgc tctggtagtg
      121 ggaaccggac aatcagcagt ccagttcgcg gagactctga aacgaacgcc ccgactgggg
      181 ttgcgtgtcg cggggttcct cgccaccgaa acggtcacca acccgtcccc gctcgcacag
      241 ccaatcctgg gccgtttgaa tgaactcggg cccgtcatcg aacaacatca ggtcaccgac
      301 gtcatcttcg ccgagccttc actgagccac aaaactgtgc tggacgccgt ggcgttgtgc
      361 agcggcagaa aagtgaattt cggtagtctc ccggacctgt acgacgtcgt ggggagaacc
      421 ggcgcagggg gacagcttta cggagttccg cttctccctc tctatccatc cgacatgccg
      481 gtctggcaac gcaggaccaa acgtgtcctc gacttcctcg ttgcgttttt cgctctggta
      541 gcgggtctgc ctctgtgggt tctggtggcg ctggcggtct ggctggagga caaaggcccg
      601 gtgttttact cccaggaccg cgtcggtaag gatggacgcg tgttccggtt ccacaaattc
      661 cgatccatga taccgaacgc ggagaaggca accgggcctg tgtgggcgca gaaaaacgat
      721 ccccgcatca cgacggtcgg atcgattatt cggaagctgc gcattgacga ggtgcctcaa
      781 ctgtggaatg ttctccgggg cgaaatgagc cttgtcggtc cccgccctga acgcccctat
      841 ttcgtcgagc gcctcgccgc ggagatcccg ctttaccgac gtcggcttct tgttacgccg
      901 ggtattaccg gatgggcaca gacgaaacat gcatatgacc agacccttga cgacgtccgc
      961 gagaagctca agtatgacct ttattacatc gagaacatgt cgctccgttt cgacttcctg
     1021 atcatactca ggacaatctg ggtgatgctg acaggcaaag gcgcgcagtg aggtattcgc
     1081 tgtcaccgtt ctcgccggtg cggtccggag cccaccgccg tccgggcgcg tgggtaagac
     1141 tgcgatggct gcgcccgggg ctttttgcgc tcggagcgat tgtcgtggcg gcgttcgtgc
     1201 tgtacacgct gctggtcgtc agaacgttac gacaggatgc ccaacgcgta gcaaaactgt
     1261 acgctgagaa aatccttccc agagcctaca gcgatcccac tatcacgccc ggggaattgc
     1321 gcctcctgtt tgacctcatt cgggagatgc cggtcgccgt aatcgtgacg gatgacaccg
     1381 gtaaccccgt gtcgtggaaa gggatcgatg ttcccgacac agcccgctcc ccgcaagcca
     1441 tggcacgcat caggcgggtg gttgaggaca tggaccggtc catgccgcct cgcaaggtgt
     1501 tggttccatt gccggataac cggtccctcc agatggagat ccacgtggcg gagagcgcat
     1561 tcctccgtcg catcgcctgg atgccggtcg tggcggcgat agtaacact
//
LOCUS       DAOSOO010000389         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_76776_length_1607_cov_5.249356, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000389 DAOSOO010000000
VERSION     DAOSOO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76776_length_1607_cov_5.249356"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLT86_15195"
     CDS             <1..512
                     /locus_tag="PLT86_15195"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOT38007.1"
                     /translation="IRRSTLLPVRYEKLQHEGWYHRDSTYVFDQDRNTVARFSRGRHV
                     GVLSLPGEVHDILSSLYRVRSSPLSVGSHVSVRVYEGGKLYNARVNVLRREQVTVPAG
                     TFSCVVVEPILQSEAIFVQRGRLWIWLTDDARRLPVLMESAIPVGTIRAELVSFLPTS
                     GTSASGSSP"
     gene            509..982
                     /locus_tag="PLT86_15200"
     CDS             509..982
                     /locus_tag="PLT86_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT38008.1"
                     /translation="MITVLTLTACGAPRSPETAPAVGERPFSFDPAEHGPGPLPEDVE
                     ETIRSLRPPPAQVDTPAAVAAPAKRDTSWTTTNGYRIQIFTSTTRELAEAEALKARER
                     FPSDSVYILFQSPWHRVRVGDFPTRQEAEEKIVEAREKGYPDAFWVRSQIRVMNR"
     gene            1266..>1607
                     /locus_tag="PLT86_15205"
     CDS             1266..>1607
                     /locus_tag="PLT86_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38009.1"
                     /translation="MDTTTACIECVDDNLIRARLYHIAKDPIPCRTLVYTLPGHEKCT
                     LHETDDFIAQSLEGWGYAVLREPVQVQAYRRNWDVEGGATYAAPQPADPWYTAYNVYG
                     MVRGSTHPQELI"
BASE COUNT          297 a          526 c          496 g          288 t
ORIGIN      
        1 cgatccggcg atcaacgctc ctccccgtcc gctacgagaa actccagcac gagggctggt
       61 accaccgcga cagcacctac gtcttcgatc aggaccggaa taccgtcgca cggttttccc
      121 gcgggaggca cgtgggcgtg ctctcgttgc cgggagaagt gcacgatatc ctctcctcgc
      181 tgtaccgcgt ccgcagttcg ccgctttcag tgggttccca tgtgtctgtg cgcgtgtacg
      241 aaggcggcaa actgtacaat gcgcgggtga acgtgctgcg ccgggaacag gttacggttc
      301 ccgccggcac cttttcctgt gttgttgtcg aaccgatatt acaatctgag gcgatttttg
      361 ttcagagagg gcgactgtgg atctggctta ccgacgacgc acgaaggctt cccgtgttga
      421 tggaaagcgc gattccagtg ggaacaattc gcgcggaact tgtttcgttc ctgccgacgt
      481 ccggcacgtc ggcgtccggt tcctcgccgt gataaccgtg ctcacgttaa cggcttgcgg
      541 cgcaccccgg tcccctgaaa ccgcacctgc ggtgggagaa cgcccctttt ccttcgaccc
      601 ggcggaacat ggtcccggcc cgttgcccga ggatgtggaa gagacgatcc ggtcgctgcg
      661 ccccccgccc gcccaggtgg atacccccgc agcagttgcc gcgccggcaa aacgcgacac
      721 gtcgtggaca acgaccaacg gctaccgcat ccagatattc acttcaacca cgcgggagct
      781 tgcggaggcg gaagcgctga aggcgcggga acggttcccc tcggattccg tctacatcct
      841 gttccagtcg ccgtggcacc gcgttcgcgt gggggatttt ccgacgaggc aggaggcgga
      901 ggagaagatc gtcgaggcgc gcgagaaagg ctatcccgac gcgttctggg tgcgcagcca
      961 gatacgcgtg atgaacaggt aagggatgcc cgccgcgagc acgagattct tcgccttcca
     1021 tcactgcgtt cgctgggctc agaatgaccg tagtctcggc ggagtgcaaa acgggcgcag
     1081 ttctgcgggg cgcccgcacc gcgtgagcgc agcgagaacc tccctcacca taagccagcg
     1141 ggccaacgac tgttccgcga acgccacgcg cacgaccgtt ttcaccgggg ggggcttggc
     1201 acggcggccg tcccgcctca ctctttcaca tcaccacgat gtcatcgtcc cttccgggag
     1261 gccccatgga caccacaacc gcatgcatcg aatgcgtgga cgacaacctt atccgcgctc
     1321 gtctttacca cattgccaaa gatcccatac cgtgccggac gctggtgtat accctgcccg
     1381 gccacgagaa gtgcacgctc cacgagacag acgacttcat cgcgcagtcg cttgaaggat
     1441 ggggatacgc ggttctgcgc gagccggtgc aggtgcaggc gtaccgccgg aactgggacg
     1501 tggagggggg ggccacgtac gccgcgccac aaccggccga cccgtggtac accgcgtaca
     1561 acgtctacgg catggtgcgc ggctccacgc acccgcagga actcatc
//
LOCUS       DAOSOO010000390         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_76914_length_1605_cov_5.812903, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000390 DAOSOO010000000
VERSION     DAOSOO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76914_length_1605_cov_5.812903"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLT86_15210"
     CDS             complement(<1..942)
                     /locus_tag="PLT86_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT38010.1"
                     /translation="MADPRLTRVEMFQDLRKRVVAEQKKRARKRQVYLCTGAGCIASG
                     ALDVKKKLDEVMKREFVDFEIRETGCLGPCAKGPALLIQPELTFYENVHPEDAEEIVH
                     QHLKQGKIVEHLLPKNARQEPVRTEEEMDFFRNQVKIVLRNCGRIDPLKIEDYVGVDG
                     YAALVKVLTSVKPDDVIESIKKSGLRGRGGAGFPTWIKWAATRKSAGDVKYVICNGDE
                     GDPGAFMDRSVLEGDPHSIIEGMAIAAFAVGAKQGYVYVRAEYPLAVARLNHAIEQAR
                     EYGFLGKNILGTSFEFDLEIRMGSGAFVCGEETALMAS"
     gene            complement(942..1502)
                     /locus_tag="PLT86_15215"
     CDS             complement(942..1502)
                     /locus_tag="PLT86_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT38011.1"
                     /translation="MKRKQAVQSIPKEPETRTDSCEPTEEELLRSLDAVIDGYSNTEG
                     ALIPVLQSAQNLFGYLPETVLRHISTRLKLPYSQVTGVVTFYSFFSTVPRGEHVVRVC
                     LGTACYVRGGKDVLATLRKQLGINVGETTEDRKFSLAVGRCFGACSLAPAVMVDETVH
                     QRVRPAKVKDLLAMYREAKPGLREAS"
BASE COUNT          292 a          501 c          447 g          365 t
ORIGIN      
        1 ggacgccata agcgccgttt cctcgccgca cacgaacgcg ccggacccca tgcggatttc
       61 aagatcgaac tcgaatgagg taccaaggat gtttttgccc aggaagccgt actcgcgcgc
      121 ctgctcgatc gcgtggttga gccgcgccac cgcaagggga tattccgcgc ggacatacac
      181 atagccctgt tttgcgccga cggcaaacgc ggcgatcgcc atcccttcga taatgctgtg
      241 ggggtcacct tccagcacgc tcctgtccat gaacgcgcca gggtcgccct cgtctccgtt
      301 gcagatgacg tatttcacgt cgcctgcgga tttgcgcgtt gccgcccact tgatccaggt
      361 cgggaagcct gcgccgccgc gcccgcgcag ccccgatttc ttgatggatt ctatgacatc
      421 atccggcttg acggacgtca gcactttgac gagcgctgca tatccgtcca ccccgacgta
      481 gtcttcgatt ttgagcgggt caatgcgccc gcagttgcgc agcacgattt tcacctgatt
      541 gcggaaaaag tccatctcct cttccgtgcg aaccggttcc tgccgcgcgt ttttcggaag
      601 gagatgctcg acgattttgc cctgtttgag atgctggtgg acaatttcct cggcgtcttc
      661 cggatgcacg ttctcataga aagtcagttc gggctgaatc aggagagccg gtcccttcgc
      721 acagggcccg aggcatccgg tttcgcggat ttcgaagtcc acgaactccc gtttcatcac
      781 ctcatcgagt tttttcttca cgtccagcgc gccagatgcg atgcatcccg cgcctgtgca
      841 gaggtacacc tgccgcttcc gcgctcgttt tttctgctcg gcaacgaccc gtttcctcaa
      901 atcctggaac atttctacgc gggttaatct ggggtctgcc atcacgaagc ctccctcaat
      961 ccgggctttg cctctcggta catcgccaga aggtctttca ctttcgccgg tctgaccctc
     1021 tggtgcaccg tctcgtccac catcacggcc ggtgcaaggc tgcacgcgcc gaaacagcgg
     1081 cccaccgcca gcgagaactt tctgtcctcc gtcgtctctc ccacgttgat tccgagttgc
     1141 ttgcggagcg tagccagcac gtcctttccg ccgcgcacgt aacacgcggt tccgagacac
     1201 acacggacca catgctcacc ccgtggaacg gtggagaaaa acgagtagaa cgtgacgacc
     1261 ccggtcacct ggctgtaggg taatttgagg cgggtgctga tgtgccggag tacagtttcg
     1321 ggaaggtaac cgaagaggtt ctgcgcggac tggagcaccg ggataagtgc tccctccgtg
     1381 ttggagtaac cgtcgatgac ggcgtcgagg ctccgcaaca attcctcttc ggttggttcg
     1441 caggaatcgg tgcgggtctc aggttctttc gggatgctct gtactgcctg tttccgtttc
     1501 atacaagtct cccggtgagt gaaccaaacc ctggatctag tgcgacggaa ctggacgatg
     1561 tacgcgaccc cgtggtgctc tgggaaaaac gcgaatgcgt gtcag
//
LOCUS       DAOSOO010000391         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_76990_length_1604_cov_5.371853, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000391 DAOSOO010000000
VERSION     DAOSOO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76990_length_1604_cov_5.371853"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1604)
                     /locus_tag="PLT86_15220"
     CDS             complement(109..>1604)
                     /locus_tag="PLT86_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38012.1"
                     /translation="FICLVQSSNTHVGWDPRPMAQYYRRLDEDLVLYDDDKSGNHAWV
                     HARKLERIFHKHGTPVTWLIDDTVAAEQAPQIAEWHGKYGDGVSFLPRSYFYYNKRNY
                     NLTHTPEDTREILTPQYAALLDAFERAGWPVYCRTMGADQWVGSPGGVFVETAQRMGF
                     EGIWGIGYDHQTCDTSMYHMGAPWDVFKPKAGNFRIPGAGSAFWCFQWTTRDIVNTSY
                     FRPTVGSTTFSTDADDIRYNGIPRFQDDYYARLLQEYRRNLSHHDVNVFLVHQEDHDS
                     HIHGSNDVLEAFVDQVHEMETFATMDEIAAWLNRRFAPEEHPYQLIEMDDPLTCHAEM
                     QAASLVGDIPRRFAKNPSWGRGGRPNPPHLAYYGEDFMWICEQPAPVPKILYNYTKSD
                     RYPFAEDGEYPEETVPALSRVEVRQQNAAGTLTIALSFDASAAIEALPVVFWNEPLNN
                     VPEGVREGTDNTGRPCHVWRTRSALAVLVHDVREGENARTVTVALEE"
BASE COUNT          266 a          485 c          537 g          316 t
ORIGIN      
        1 gcgtggcgca ccctgcggag gcgaatgcga tgattgccgc ggccgcgtaa cgcgttccga
       61 gcaggagcgg gcggggccag ggagccgccg cgcacgcgga accgccctct actcctccag
      121 agcgacggtg acggtgcggg cgttttcccc ttcgcgcacg tcatgcacca gcacggcgag
      181 ggcggaacgg gtccgccaga cgtggcaggg ccggcccgtg ttgtcggttc cctcccgcac
      241 tccctcggga acgttgttca gcggctcgtt ccagaacacc accggcaatg cctcaatggc
      301 ggcactcgcg tcgaacgaga gggcgatggt gagagtgccc gccgcgttct gctgccggac
      361 ttccacccgg gaaagcgcgg gaaccgtttc ctcagggtat tccccgtcct ccgcgaacgg
      421 gtaccggtcg gatttcgtgt agttgtagag gatcttcggc acgggagccg gctgctcgca
      481 tatccacatg aaatcctcac cgtagtaggc aaggtgcggc ggattgggtc tgccgccgcg
      541 gccccacgag ggattcttcg caaacctccg cggaatgtcc cccaccagcg acgcggcctg
      601 catttcggcg tggcaggtga gcgggtcgtc catttcgatg agctggtacg ggtgttcctc
      661 cggcgcaaac cggcgattga gccatgccgc gatttcgtcc atggtggcga acgtttccat
      721 ttcgtggacc tgatccacga acgcctccag cacgtcgttg ctgccgtgga tgtgggagtc
      781 gtggtcttcc tggtgaacga ggaacacgtt gacgtcgtgg tgggagaggt tcctgcggta
      841 ttcctgcaac aggcgggcgt agtagtcgtc ctgaaagcgt ggtattccgt tgtaccggat
      901 gtcgtcggcg tcggtggaga aggtggtgga gccgaccgtg gggcggaagt aggaggtatt
      961 cacgatgtcc cgcgtggtcc actggaagca ccagaacgcg ctgccggcgc cggggatgcg
     1021 gaagttaccc gccttcggct tgaatacgtc ccacggcgcg cccatgtggt acatgctggt
     1081 gtcgcaggtc tggtggtcgt atccgatgcc ccagattccc tcgaatccca tgcgctgcgc
     1141 cgtttcaaca aacacaccgc caggagaccc cacccactgg tctgcgccca tggtccggca
     1201 gtacaccggc catcccgcgc gttcgaacgc atcaagcagc gcagcatatt gcggagtgag
     1261 gatctcccgc gtgtcctccg gcgtgtgagt gaggttgtaa ttgcgcttgt tgtagtagaa
     1321 atacgagcgc ggcagaaatg agacgccgtc cccgtacttc ccgtgccatt cggcgatctg
     1381 cggcgcttgc tctgccgcca cggtgtcgtc tatcagccag gtgaccgggg tgccgtgttt
     1441 gtggaaaatg cgctcgagct tgcgggcgtg cacccaggca tggttgccgc tcttgtcgtc
     1501 gtcatagagg acaaggtctt catcgaggcg gcggtaatac tgcgccatgg ggcggggatc
     1561 ccatcccacg tgcgtgttgc tcgattgcac gaggcagatg aagc
//
LOCUS       DAOSOO010000392         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_77956_length_1590_cov_4.261889, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000392 DAOSOO010000000
VERSION     DAOSOO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77956_length_1590_cov_4.261889"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLT86_15225"
     CDS             complement(<1..1173)
                     /locus_tag="PLT86_15225"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT38013.1"
                     /translation="MPKYAVPYAAPPVLERPSNAQFSLGGVVGERVKAALHGWLLPAP
                     DANPALTEMFRDRNRTSSRELVPWAGEFVGKYLIAAQQILRLTGDKRLRSTIGGIVAE
                     LIAAQDDDGYLGPFDKENRLVEKWDVWGHYHVMLALLMYYEASGSLFALSACRKMADL
                     LHSFFVVGNRRMLTRDDPDGEKNYAVSHAVLWLYRITGEAKYRELVDWIEKEWDVPPA
                     GQYISSALAGVPVWKFPSHRWESAHGYEAIAELGLLTGDERYARVARHIWWSILEGDR
                     HNTGGFTSGEACQGNPYDQGAIETCCTMAWIAFSIDVLRMTGDPRIADEIELATLNGM
                     VGGQNPAGRWWTYNTPMDGRRVASAQDIVFQSRAGSPELNCCSVNAPRGLGMISEWG"
BASE COUNT          270 a          555 c          495 g          270 t
ORIGIN      
        1 cccccactcc gagatcatcc ccagcccgcg cggcgcgttc accgagcagc agttgagttc
       61 cgggctgcca gcgcgcgact ggaacacgat gtcctgcgcc gaggccacgc gccggccatc
      121 catcggcgtg ttgtacgtcc accaccgccc cgccgggttc tgcccgccca ccattccgtt
      181 cagcgtcgcc agttcgatct cgtccgcaat ccgcgggtcg ccggtcatcc tgagcacgtc
      241 gatggagaac gcgatccagg ccatggtgca gcaggtctcg atggcgccct gatcatacgg
      301 gttgccctgg cacgcctcgc cggaggtgaa cccgccggtg ttgtgccggt cgccctcgag
      361 gatgctccac cagatgtgcc gggcgactcg cgcataccgc tcgtcgccgg tcagcagccc
      421 cagttcggcg attgcctcgt aaccgtgcgc gctttcccac cggtgcgagg ggaacttcca
      481 caccggcacg ccggccagcg cggagctgat gtactgcccg gcgggtggca catcccattc
      541 cttctcgatc cagtccacca gctcgcggta tttcgcctcg ccggtgatgc ggtacagcca
      601 cagcaccgcg tgcgacaccg catagttctt ctcgccgtcg ggatcgtctc tggtgagcat
      661 ccgccgattc cccaccacga agaaggagtg cagcagatcg gccatcttgc ggcaagcaga
      721 gagtgcaaag agcgacccgg aggcctcgta atacatgagc agcgccagca tgacgtggta
      781 atgcccccac acatcccact tttccacgag gcggttttcc ttgtcgaacg ggccgaggta
      841 gccgtcgtcg tcctgcgcgg cgatgagttc ggcaacgatg ccgccgatgg tggagcggag
      901 cctcttgtcg ccggtcagac gcagtatctg ttgcgcggcg atgaggtatt tacccacaaa
      961 ctcacccgcc cacggcacaa gctcccgcga ggacgtccgg ttgcggtcgc ggaacatctc
     1021 ggtcagcgcg gggttggcat cgggcgcggg caggagccag ccgtggaggg cggccttcac
     1081 ccgctcgccg acaacaccgc cgagcgaaaa ctgcgcattc gatggccgtt caagtaccgg
     1141 cggcgcggca tagggaacag cgtatttcgg catacggaaa acccccgatg atgggtgaaa
     1201 atgcgcgcaa tggccggagt tgctcccgcg gtcactgagc ctgtcgaagt gccgccaggc
     1261 agattgccgc ccaccgggcg accttccgaa gccccgcgcg gcgtttcgac aagctcaacg
     1321 accgcgctgg ccctagaggg atccgcggag aacaccgttc gttcttcgcc aggtcccctt
     1381 ttttcccacg cccgggacgt tgccccgggc tgagctattc cggcccttca ggccttctgg
     1441 cgccggtgaa ggcgggcgcc catcctgcga ggcccctgag cctgcggggc cgcaggaagc
     1501 ctgtcgaagt gccgtcgcga tgcattgaaa cgccgtgcag actggcgcca gcaaggtcat
     1561 tggtcacgac agagcgtgac cctccaaccc
//
LOCUS       DAOSOO010000393         1587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_78174_length_1587_cov_5.603133, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000393 DAOSOO010000000
VERSION     DAOSOO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78174_length_1587_cov_5.603133"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1587
                     /locus_tag="PLT86_15230"
     CDS             <1..>1587
                     /locus_tag="PLT86_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT38014.1"
                     /translation="EPERWVSSTEVLFGGAASSYDFEGKLFAVHTPEGKYGLLLFRYP
                     QMGDKPARDSVATASAARAGRTQNGDGTLYAHWAYQPDGTTRFGRAQGPFAMPNLVGM
                     TQGEATAQLTLLGFGQINVRYVGTTTPSLIGKVKEQEPAAGQSAYPEWPVTLTVWAQQ
                     GQKVQILGVTPANGAVNVGVPAGPDSVLVTITVALSGGVELIPGDNGPQPNVDIMVAP
                     DITGVPVNYTWNAARDTLRYAIRLPKNRVFDVLVTPSENDDFGVELANSAFFMSSFST
                     GPDFSGARLSGTASIPNMNMPARVALLLNRAVAEFGEFGEHVIERIVPISQTGAYSFT
                     GLTAGRYWLVGMSFDPATMEGGSGTMPEVYYGMYDLYGDRLPDPLDFKSSTVRENINF
                     ALRRLTAQDFVELRVVATTPDPFTTGVPTSTTLRVRFNEQLNVVGGKPDVRVALVSPS
                     LPVRFLSSDSVFFSADRKEVWWNVQLAASTHYQIFVTDAGAAGENRKFRIPVAVPFAT
                     SSFLPSGSLNAQMIPPPGGAS"
BASE COUNT          288 a          476 c          507 g          316 t
ORIGIN      
        1 ggagccggag cgctgggtat ccagcacgga ggtgctgttc ggaggtgccg cctcttccta
       61 tgactttgaa ggcaagcttt tcgcggtaca cacacccgag ggaaagtacg ggctgctcct
      121 gttccggtac ccgcagatgg gcgacaaacc ggctcgcgac tcagtggcga cagcgtcggc
      181 ggcacgtgcg gggcggactc agaatggcga tggcacgctc tacgcgcact gggcgtacca
      241 gcctgacggc accacccgtt tcggacgggc acaggggccg tttgcaatgc cgaatctggt
      301 cgggatgacg cagggcgaag ccacggccca gctcacgctt ctcggcttcg ggcagataaa
      361 cgtgcggtat gtcggaacaa cgactccctc gctcatcggg aaggtgaagg aacaggaacc
      421 ggcagcaggc caatcagcgt acccggaatg gccggtgacg ctcaccgtct gggcgcagca
      481 ggggcagaag gttcagattc tgggagtgac gcccgctaat ggtgcggtga acgtgggcgt
      541 accggcagga ccggacagtg tcctggtcac catcactgtc gcactgagcg gcggggtgga
      601 gctcattccc ggcgataacg gcccgcagcc gaacgtggat atcatggttg cgccggatat
      661 cacgggtgtt cctgtcaact acacgtggaa cgcggcacgg gacactttgc gctatgccat
      721 ccggcttccg aaaaaccgcg tgttcgacgt gctggtcacc ccttccgaga atgacgattt
      781 cggagtcgag ttggcgaact cggcgttctt catgtcgtcg ttctcgaccg gccccgattt
      841 ttcgggtgcg cggctcagcg ggactgcttc gatccccaac atgaacatgc cggcacgcgt
      901 cgccctcctg ctgaaccggg ccgttgccga gttcggggaa ttcggtgagc atgtgattga
      961 gcggatcgtg ccgatttccc agacgggggc atactcgttc accgggctca ccgcggggcg
     1021 atactggctt gtcggcatga gtttcgatcc ggccacgatg gaaggcgggt cggggacgat
     1081 gccggaggtc tactacggca tgtacgatct ctacggcgat cggctgccgg atccgctgga
     1141 cttcaagtcc tcgacggtgc gggagaatat caattttgca ctgcggcggt tgactgctca
     1201 ggacttcgtc gagttacgcg ttgtagccac cacgcccgat ccattcacga ccggcgtgcc
     1261 cacgagcacg accctacgtg ttcgcttcaa cgaacaactc aacgtggtag gcgggaaacc
     1321 ggacgtccgc gtcgcgctcg tttcgccgtc gcttcccgtc aggttccttt cttctgacag
     1381 cgtgtttttc agtgctgacc gaaaagaggt ctggtggaac gtccaacttg ctgcgagtac
     1441 gcattatcag atcttcgtta ccgacgccgg agctgccggc gagaaccgca agttccgcat
     1501 tccagtcgcg gtgccgtttg cgacttcgtc gtttctgcca tccggcagcc tgaacgcgca
     1561 aatgattccc cctccgggcg gagcctc
//
LOCUS       DAOSOO010000394         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_79081_length_1574_cov_4.516129, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000394 DAOSOO010000000
VERSION     DAOSOO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79081_length_1574_cov_4.516129"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLT86_15235"
     CDS             complement(<1..1038)
                     /locus_tag="PLT86_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT38015.1"
                     /translation="MTSRERLLTAIHNDTPDRVPVGPFDLGRLPRDSPIARDLIRTTD
                     PFIQCGLGGDPIWGKNCPTERIQDGRDRITIVHTPLGDMRSVWRSTDVASATVEYFCS
                     TPEDAEKWLSIPYTPPEPDLADFYRVKQEIGEEGLVLAGMGDAICLPASLYSPEDFCL
                     LWLDAPDVMRRLVQTAANRINAYIERACAQGMDAFRIVGGEYASVQLGPEAFQELVVE
                     PDRELCDIMRRHGAVAYYHNHGPVTRFYDQFVEIGMHALDPMEAPPWGDCNLEEAKQK
                     LGGKVCLVGNLDDMEIMESLEWSEIEPVASERLLQAGPDGFVLGGTASGTYGEHAARM
                     FMKLVPLAETMA"
BASE COUNT          339 a          483 c          445 g          307 t
ORIGIN      
        1 agccatggtt tcggcgagcg gaacgagttt catgaacatc cgcgccgcgt gttcgccata
       61 cgtgcccgac gccgtacccc cgagaacgaa cccgtcagga cccgcctgca gaaggcgttc
      121 cgaagcgaca ggttctattt cgctccactc cagcgattcc atgatttcca tgtcgtccag
      181 attgccgacg agacagacct tgcctccgag tttctgtttg gcttcttcga ggttacaatc
      241 cccccaggga ggtgcttcca tcggatcgag ggcgtgcatg ccgatttcca cgaactggtc
      301 gtagaagcgc gtgaccggac cgtggttgtg atagtacgcg accgcaccgt ggcgccgcat
      361 gatgtcgcac agctccctgt ctggttcgac caccagttcc tgaaacgctt cggggcccag
      421 ttgcacgctg gcatattcgc caccaacgat tctgaacgca tccataccct gggcgcatgc
      481 acgctctatg tacgcgttga tccggttcgc agccgtctgc acaagcctgc gcatgacgtc
      541 aggagcgtcc agccaaagga gacagaagtc ctcgggggaa taaagggatg cgggaagaca
      601 tatcgcgtcg cccatgcccg caaggacgag gccctcctcg cctatttcct gtttgacacg
      661 atagaaatcc gcaagatcgg gctctggcgg agtgtaggga atcgacaacc atttctctgc
      721 atcttccggg gtgctgcaga agtactcaac ggttgctgac gcaacgtcgg tactccgcca
      781 gacggaacgc atatccccca gcggcgtgtg gactatcgtg atacggtccc ttccgtcctg
      841 aattcgctcg gtcgggcagt tttttcccca gattggatcc ccacccaatc cgcactggat
      901 gaacggatca gtggtccgta tcagatcacg cgcgatcgga gaatcgcgtg gaaggcgccc
      961 aaggtcaaaa gggccgaccg ggacacgatc tggagtatca ttgtggatag cagtcagaag
     1021 gcgctcgcgg gaagtcatgg gaagctccac aggaaacaat gccggaagca gagggaaagt
     1081 aatctgaacc taaatcatcc ggcctctaga gcgcaaccga ggggtaggca cagtttgttc
     1141 cggagcagcg gcacaaatga aacgccggcg gaagggaggg acacctcccg tccaccggcg
     1201 caaattggtc atccgccacc cgttccctgt tccgcaccgc caacgcccgc ggagtttccg
     1261 cgggtacccc gtcaagggaa tccggatgac gaaagcggca tacgcgccaa gggtttttcc
     1321 ccggcaaaca agccaccgaa ccaaccaata gcaacaatca tacccgtgtt agtccgatgc
     1381 ggccgattcg ctgtttcaaa cgcccgtgtg cggactgctt caccgtacga ttccgtacgg
     1441 cagtgcgcgg tttggaacac gtcgccccgg cactctgacg tgaatcgtca tcctgtgcgt
     1501 cttacgccgg gatacgcctg gtcaagtcct gctctcagaa ctgatccgtc acgcagcctt
     1561 ctgatgcggg cgaa
//
LOCUS       DAOSOO010000395         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_83159_length_1519_cov_4.717213, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000395 DAOSOO010000000
VERSION     DAOSOO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83159_length_1519_cov_4.717213"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="PLT86_15240"
     CDS             <1..1368
                     /locus_tag="PLT86_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT38016.1"
                     /translation="VRRTNNEFLGVNTLLRVSSVEPSVLKWTASYCYLHTNNHLPGNS
                     AYHDLPNTARLLSGQHLAEGMVRIPLPLLTEAEIRGYGSSRRDRFENKDGTVGLGRSV
                     TNDRTAAWGGQLTLSSLIVPLQRISLFSQVAREAYYPDDRMITDPAVRQKLLRSSRRA
                     QYSFYLSDEIALLQRRIVLTGNVGTHTVSNNLIREETGSRTWSDSTSFTQHPHAVGIV
                     VAPTPWVSIRANLGRYTRVPNLFELYGNRGTSVGNSLLVPERGENRDIGFVAHTTRED
                     GRLRHASAEVVLFESKITDMIAFWSVYNRMKPFNVGKAQIRGVEVAGSLATAFGFSSN
                     LGVTWQHPRNESALYNGLYFGNDLPHRPRWQIDSRSEFSFSGVTLAYGLHYHNRFFGQ
                     PVNSIGDTMPSAFLHDVGVRFRPHERVHLSIEGKNLGNVREFHSRYVPLPGRSVFVAC
                     QVSSS"
BASE COUNT          320 a          444 c          410 g          345 t
ORIGIN      
        1 gtgcgacgga cgaataacga gtttctcggt gtcaacacgc ttctgcgcgt ttcctctgtt
       61 gaaccatcgg tactgaaatg gaccgccagc tactgctacc tgcacacgaa taaccacctg
      121 ccgggaaata gcgcctatca tgatctgccc aacactgcgc gcttgctttc cgggcagcat
      181 ctcgccgaag ggatggtgag gataccctta ccacttctga cggaagcgga gatccggggg
      241 tacggttcat cccgtcgtga tcgttttgag aacaaagacg gaacggtcgg tctgggcaga
      301 agcgtcacca atgaccgtac cgccgcatgg ggggggcaac ttaccctgtc atcgctcata
      361 gttcccctgc agcgtatttc cttgttttcc caggttgcgc gcgaggctta ctaccccgat
      421 gacaggatga tcacagaccc tgcagtccgg cagaagttgc tccgttcgag ccggagggcg
      481 caatactcct tctacctctc cgatgaaatc gctttgctcc agcggagaat tgtacttacg
      541 ggcaatgtcg gaactcatac agtcagcaac aatctgattc gggaggaaac gggctcgcga
      601 acgtggtccg attccaccag ttttactcag catccgcacg cagtcggcat tgttgttgcc
      661 ccgactccct gggtttcgat tcgcgccaat ttgggacgct acacccgggt tccaaacctt
      721 ttcgagcttt acgggaacag aggcacgagc gtaggaaact ccttactggt tcccgaacga
      781 ggcgaaaatc gtgacatagg ttttgtcgca cacacgaccc gcgaagatgg tcgccttcgg
      841 catgcctccg cggaggtggt tctctttgag agcaagatta ccgacatgat cgcattctgg
      901 tcggtgtaca accgcatgaa gcccttcaac gtggggaagg cacagattcg cggggtggaa
      961 gtagccggtt ccctcgcaac cgcattcggg ttttccagca atctcggcgt aacgtggcag
     1021 cacccgcgca acgaatccgc gctctacaac ggcctctact tcgggaacga cctgccccac
     1081 cgaccacgat ggcaaatcga ttcgcgcagt gagttctcgt tctctggcgt aacgctggcg
     1141 tatggacttc attaccacaa ccgatttttc gggcaaccgg ttaacagcat cggtgacacg
     1201 atgccgtctg cgttcctcca cgatgtggga gttcgtttcc gtccacacga acgagtgcat
     1261 ttgtcgatag aaggcaagaa cctgggaaat gttcgggaat ttcacagtcg ctacgtgccc
     1321 cttccagggc gaagcgtctt cgttgcctgt caggtttctt cgtcctgaca gagcgttctg
     1381 ttcgttacca aaaggaggtc ccatgctcac aaaggttgta cgccgactca ttcacacgtc
     1441 cgcgactgcc tcgattcttt tcgtgctcgc gtcatgcgga acgaataacc ctgcccggtc
     1501 gcacagtgcc gggtacggg
//
LOCUS       DAOSOO010000396         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_83577_length_1514_cov_2.644277, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000396 DAOSOO010000000
VERSION     DAOSOO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83577_length_1514_cov_2.644277"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLT86_15245"
     CDS             <1..632
                     /locus_tag="PLT86_15245"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38017.1"
                     /translation="YPHFILTQMPTFVAGVVISAVLAAAMSSVDSSMNASAAVLLSDF
                     YKRHLAPNRTDTHYLKVARFITAGLGAAMMVVALGLSSLEASTILEVSFFVSAVITGG
                     LGGFFLLGFLITRANDQGARIGVAAGVFSILWCTLSSLQPALGLLPTWAVIRVHPFLI
                     GVVGNFVIFAVGVAASFFFPRPTEQQLAGMTWWTRDRRSEKEALEQAGR"
     gene            823..>1514
                     /locus_tag="PLT86_15250"
     CDS             823..>1514
                     /locus_tag="PLT86_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38018.1"
                     /translation="MGLRLGDAIAIALYVAAMVWLGWRSQRRIKGTEGYFVGNRAMPG
                     WAVGLSILATAISSVTFLAMPGNSYQGNWSRYVPGLMLPVATLIGVYFFVVFYRRTRF
                     VSAYQYFEKRFGNWGRSYASVLFSIGSLYRIGMVLYLMSIPIKVLFGGDILSIILFAG
                     IVATVYTVMGGLEAVIWTDVVQGGVLVLGGVLTIAIVFLDVPGGGAEIIHRASAAGKF
                     DMAASFDWSLSR"
BASE COUNT          242 a          450 c          470 g          352 t
ORIGIN      
        1 tctatcccca cttcatcctc acccagatgc ccactttcgt ggcgggagtt gtgatctccg
       61 ccgtactggc ggccgcaatg tcgagtgtcg attccagcat gaacgcctcg gcggcagttc
      121 tgctgtcgga tttctacaaa cgacaccttg cgccgaaccg gaccgacacg cactacctca
      181 aagttgcccg attcatcacc gcaggactgg gcgctgcgat gatggtggtt gcgctcggac
      241 tctcatcgct tgaagccagc acgatcctcg aagtcagctt tttcgtcagc gcggtgataa
      301 ccggaggcct gggcgggttc ttcctcctcg ggttcctcat cacgcgcgca aacgatcagg
      361 gtgcacggat aggtgttgcg gcgggcgtgt tctcaattct ctggtgcacg ctcagcagtc
      421 ttcagcctgc tctggggttg ctcccgacgt gggccgtgat ccgggtgcac ccgttcctga
      481 tcggcgtggt gggaaatttc gtcattttcg cagttggcgt cgccgcgagc ttcttcttcc
      541 cgcgtccgac agagcagcaa ctcgccggta tgacgtggtg gacgcgcgac aggcgttcgg
      601 aaaaggaagc gctggagcag gccggccgct gatttcccgt gcgtgcgctc aggacgaagc
      661 acgcctcgct ccatgtgccg gatccaaccg cggacaacgg aaatctccgg cagcgccatg
      721 ctgttgcgcc accgcgcagc gcggggtgat cttgttgttt cctgccctgc acatgaatac
      781 cgctcgcgcg ttcgaggaac cattcgttcg gggaggtgac cgatgggact tcggttaggg
      841 gatgcaattg ccatcgcgct gtacgtcgcc gccatggtgt ggctcggttg gcgatctcag
      901 cgccggatca aaggaaccga agggtatttc gtcggcaatc gggcgatgcc ggggtgggca
      961 gttggccttt cgatcctggc gacggcgata agctcggtta cgttcctcgc gatgcccggg
     1021 aattcctacc agggcaactg gtcgcgttac gtccccggcc tgatgcttcc tgtggccacc
     1081 ctcatcgggg tgtatttctt cgtcgtcttc taccgccgca cacgcttcgt cagcgcgtac
     1141 cagtatttcg agaaacgctt tggcaactgg ggtcgctcct acgcaagtgt attgttctcc
     1201 atcggttctc tctaccgcat aggcatggtg ctgtacctga tgtccatccc gatcaaggtt
     1261 ctgttcgggg gagacattct ctccatcatc ctgttcgccg gcattgtcgc gacggtgtac
     1321 accgtgatgg gtgggcttga ggcggtgatc tggaccgacg tggttcaggg gggcgttctt
     1381 gttctgggcg gcgtgctcac catagccatc gtgttcctcg atgtgccggg cggtggcgca
     1441 gagatcatcc accgagcgtc agcagccggg aaattcgaca tggcggcttc gttcgattgg
     1501 agccttagcc ggga
//
LOCUS       DAOSOO010000397         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Latescibacterota bacterium isolate
            AMR_MDS_2343 NODE_83860_length_1510_cov_3.996564, whole genome
            shotgun sequence.
ACCESSION   DAOSOO010000397 DAOSOO010000000
VERSION     DAOSOO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864410
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Latescibacterota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Latescibacterota bacterium
            Bacteria; Candidatus Latescibacterota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:00:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 3,007
            Genes (coding)                    :: 2,993
            CDSs (with protein)               :: 2,993
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Latescibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83860_length_1510_cov_3.996564"
                     /isolate="AMR_MDS_2343"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053570"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLT86_15255"
     CDS             <1..508
                     /locus_tag="PLT86_15255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT38019.1"
                     /translation="IALKDSIVNRLRIATIRECRQSDVISVRRLIHETIDASYAGVYP
                     PRALQSFREFHSDENIAERVRTGTVLVLKREGEIVATGSLVGNEICGVFVRASCQRCG
                     YGRVVMVELEKRAKVNGCARGELSVSLPSRAFYESLGYEITEECSMDVGEGQRLDYWR
                     GRKLLSKG"
     gene            complement(553..1173)
                     /locus_tag="PLT86_15260"
     CDS             complement(553..1173)
                     /locus_tag="PLT86_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT38020.1"
                     /translation="MELENGKRELRSVGRDRVTPIRRCLRLPGYDYSQAGAYFVTLCA
                     HHRECLFGEVLDGRMRMNAAGEMVARWLSEVQYKFPNAKIDAHIVMPNHVHAIVVLRG
                     AVGADLRVRPPEETVTNADGDTTGAHKGAPLPRIVQWIKTMATNGYMHGVESQGWPPF
                     PGRLWQRSYYDHIIRDENDLRRAREYIEMNPARWAEDEEYTGVPCV"
     gene            complement(1181..>1510)
                     /locus_tag="PLT86_15265"
     CDS             complement(1181..>1510)
                     /locus_tag="PLT86_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT38021.1"
                     /translation="REFGAALRRRYGAPLAETSGRGNAVELPLPRPAVVDRAILMEEI
                     TQGERIREYVLEGRTDAGWAPLARGQSIGHKRIERFPATSVSAVRLRVEKAIAEPWVR
                     RLAVFEE"
BASE COUNT          257 a          471 c          492 g          290 t
ORIGIN      
        1 aatcgcgctg aaggactcga tcgtgaaccg acttcgcatc gccacgatcc gggaatgccg
       61 gcagtcggac gtaatttcag tccggcggct gattcacgag acgatcgacg cgtcctacgc
      121 gggcgtgtat cctcctcgcg cgctgcagtc gttccgggag tttcactcgg acgagaacat
      181 cgcggaacgg gtgagaaccg gaacggtgct tgtccttaag cgggaggggg agattgtcgc
      241 cacggggagc cttgtcggca acgagatatg cggcgtgttc gtgcgcgcat cctgccagcg
      301 atgcgggtac ggccgggtgg tgatggtgga attggagaaa cgggcgaagg tcaacggctg
      361 cgcgaggggg gaacttagcg tgtccctgcc gtcacgggcg ttctacgaga gccttggcta
      421 cgagatcacc gaggaatgct cgatggacgt gggggaggga cagcgcctag attactggcg
      481 ggggaggaag ctgctgagca agggataacc ggccggtcgt cgcccgccgc cgacgcgtac
      541 ctcgcctctc gatcagacgc acgggactcc ggtgtactcc tcgtcttccg cccaccgtgc
      601 ggggttcatt tcgatgtatt cgcgggcacg tcggaggtcg ttttcatctc ggatgatgtg
      661 gtcgtaataa gagcgttgcc agagccgtcc tgggaatggt ggccatccct gggattcgac
      721 accatgcatg tatccgttcg tggccatcgt tttgatccat tggacaattc gcggtagggg
      781 cgcacctttg tgtgcgcccg tggtatcgcc gtcggcattt gtgaccgttt cttcgggcgg
      841 gcggacacgc aggtccgccc ctacggcacc ccgcaaaacc acgatggcgt ggacatgatt
      901 cggcatcacg atgtgtgcgt cgatcttcgc gttggggaat ttgtactgca cttctgacaa
      961 ccaacgggcc accatctcgc ccgcggcgtt catccgcatc cgcccgtcca acacctcgcc
     1021 gaaaaggcat tcacggtggt gggcgcacaa cgtgacgaag tacgctccgg cctgtgagta
     1081 atcgtaacct ggcaaccgga gacatcggcg gatcggagtt acccggtcgc ggcccacgct
     1141 gcgaagctcg cgtttcccgt tttccaactc catcgtgtgt tcactcctca aacaccgcca
     1201 gccgcctcac ccacggctcg gcgattgcct tctccacgcg caggcgaacg gcggataccg
     1261 atgtcgcggg gaaccgctca atgcgcttgt gtccgatgct ctgcccccgc gcaagcggcg
     1321 cccacccggc gtccgttctg ccctcgagga cgtattcgcg gatgcgctcg ccctgcgtga
     1381 tctcctccat caggatggcg cggtccacca cggcagggcg gggaagcggc agttcgacgg
     1441 cattgccccg gcccgacgtc tccgccagcg gggcgccgta ccgccggcgc agggccgcgc
     1501 cgaattcgcg
//
